WorldWideScience

Sample records for pimpinella anisum foeniculum

  1. Fitoterapide karminatif olarak kullanılan Apiaceae Meyvaları (Foeniculum vulgare, Pimpinella anisum)

    OpenAIRE

    2006-01-01

    Bu çalışmada halk arasında karminatif olarak kullanılan Apiacea bitkileri Foeniculum vulgareve Pimpinella anisum'un kimyasal özellikleri ve biyolojik etkileri literatür verilerine dayanılarak incelenmiştir.Pimpinella anisum uçucu yağının büyük kısmını anetol oluşturmaktadır. Uçucu yağda ayrıcaöstragol, kumarin yapısında bileşikler (bergapten, umbelliferon ve skopoletol), lipitler(petroselinik asit, palmitik asit, linoleik asit) ve flavonoitler de bulunmaktadır.Pimpinella ...

  2. The insecticidal potential of Foeniculum vulgareMill., Pimpinella anisum L. and Caryophillus aromaticus L. to control aphid on kale plants

    OpenAIRE

    P.S.R Lucca; L.H.P. NÓBREGA; ALVES, L.F.A.; Cruz-Silva,C.T.A.; PACHECO,F. P.

    2015-01-01

    ABSTRACTThe use of natural substances for pest control in agriculture is, economically, a viable option and has benefits for both the humanbeing and the environment, due to its low persistence and toxicity. Thus, this trial aimed on determining the insecticidal potential of the extracts and essential oils of fennel (Foeniculum vulgare Mill.), anise (Pimpinella anisum L.) and clove (Caryophillus aromaticus L.) to control Brevicoryne brassicae L. in kale (Brassica oleracea var. acephala DC.). T...

  3. Randomized clinical trial of a phytotherapic compound containing Pimpinella anisum, Foeniculum vulgare, Sambucus nigra, and Cassia augustifolia for chronic constipation

    Directory of Open Access Journals (Sweden)

    Amaral Karine M

    2010-04-01

    Full Text Available Abstract Background A phytotherapic compound containing Pimpinella anisum L., Foeniculum vulgare Miller, Sambucus nigra L., and Cassia augustifolia is largely used in Brazil for the treatment of constipation. However, the laxative efficacy of the compound has never been tested in a randomized clinical trial. The aim of this study was to evaluate the efficacy and safety of the product. Methods This randomized, crossover, placebo-controlled, single-blinded trial included 20 patients presenting with chronic constipation according to the criteria of the American Association of Gastroenterology. The order of treatments was counterbalanced across subjects: half of the subjects received the phytotherapic compound for a 5-day period, whereas the other half received placebo for the same period. Both treatment periods were separated by a 9-day washout period followed by the reverse treatment for another 5-day period. The primary endpoint was colonic transit time (CTT, measured radiologically. Secondary endpoints included number of evacuations per day, perception of bowel function, adverse effects, and quality of life. Results Mean CTT assessed by X ray was 15.7 hours (95%CI 11.1-20.2 in the active treatment period and 42.3 hours (95%CI 33.5-51.1 during the placebo treatment (p Conclusions The findings of this randomized controlled trial allow to conclude that the phytotherapic compound assessed has laxative efficacy and is a safe alternative option for the treatment of constipation. Trial registration ClinicalTrial.gov NCT00872430

  4. The insecticidal potential of Foeniculum vulgareMill., Pimpinella anisum L. and Caryophillus aromaticus L. to control aphid on kale plants

    Directory of Open Access Journals (Sweden)

    P. S. R. LUCCA

    2015-12-01

    Full Text Available ABSTRACTThe use of natural substances for pest control in agriculture is, economically, a viable option and has benefits for both the humanbeing and the environment, due to its low persistence and toxicity. Thus, this trial aimed on determining the insecticidal potential of the extracts and essential oils of fennel (Foeniculum vulgare Mill., anise (Pimpinella anisum L. and clove (Caryophillus aromaticus L. to control Brevicoryne brassicae L. in kale (Brassica oleracea var. acephala DC.. The treatments were: fennel, anise, cloves extracts at 10%; fennel, anise, cloves oils at 1% and control with distilled water. The mortality tests were carried out with aphids in laboratory, with three replications, after 1, 12, 24, 48 and 72 hours. During laboratory trials , it was found out that fennel oil at 1% showed the best rate of mortality on aphid nymphs (70% at 72 h, followed by clove extract at 10% with 37% mortality. Tests in pots were only carried out only with cloves extracts at 10% and fennel oil at 1% treatment, in which such efficiency was alsoindicated on aphid nymphs.

  5. Review of Pharmacological Properties and Chemical Constituents of Pimpinella anisum

    OpenAIRE

    2012-01-01

    Pimpinella anisum (anise), belonging to Umbelliferae family, is an aromatic plant which has been used In Iranian traditional medicine (especially its fruits) as carminative, aromatic, disinfectant, and galactagogue. Because the wide traditional usage of Pimpinella anisum for treatment of diseases, in this review published scientific reports about the composition and pharmacological properties of this plant were collected with electronic literature search of GoogleScholar, PubMed, Sciencedirec...

  6. Burdur İli Anason (Pimpinella anisum L., Kişniş (Coriandrum sativum L. ve Rezene (Foeniculum vulgare Mill. Agro-Ekosistemlerinde Böcek Biyolojik Çeşitliliklerinin Belirlenmesi

    Directory of Open Access Journals (Sweden)

    Gökhan AYDIN

    2014-02-01

    Full Text Available Bu çalışma, Burdur ilinde kültürü yapılan önemli tıbbi ve aromatik bitkilerden pestisit uygulaması yapılmayan ve periyodik olarak münavebe yöntemi uygulanan anason (Pimpinella anisum L., kişniş (Coriandrum sativum L. ve rezene (Foeniculum vulgare Mill. agro-ekosistemlerinde böcek biyolojik çeşitliliklerinin araştırılması amacı ile böcek aktivitesinin yoğun olduğu 2009 yılı nisan ve eylül ayları arasında yapılmıştır. Çukur tuzak örnekleme yönteminin uygulandığı habitatlardaki böcek biyolojik çeşitlilik değerleri Shannon-Wiener ve Simpson çeşitlilik, Simpson dominantlık, Shannon Populasyon yoğunluk ilişkisi parametreleri kullanılarak hesaplanmıştır. Buna göre hesaplanan biyolojik çeşitlilik indekslerinde rezene agro-ekosisteminde hem Shannon-Wiener hem de Simpson çeşitlilik indeks değerleri sırası ile 2.5838 ve 0.8742 ile diğer agro-ekosistemlere göre en yüksek bulunmuştur. Simpson dominantlık parametre sonucuna göre ise 0.2025 değeri ile kişniş agro-ekosistemi, anason ve rezene agro-ekosistemlerine göre dominantlığın en yüksek olduğu tarım alanı olarak belirlenmiştir. Habitatlardaki populasyon yoğunluk ilişkisi incelendiğinde Shannon Evenness değeri anason agro-ekosisteminde en dengeli olarak bulunmuş ve 0.7333 olarak hesaplanmıştır. Bu sonuçlara göre pestisit uygulaması yapılmayan ve periyodik olarak münavebe yöntemi uygulanan agro-ekosistemlerdeki böcek türleri ve birey sayılarından elde edilen çeşitlilik değerlerinin doğal habitatlara benzerlik gösterdiği ortaya çıkartılmıştır. Anahtar kelimeler: Tür Zenginliği, Shannon-Wiener Çeşitlilik, Simpson Dominantlık, Populasyon Yoğunluk İlişkisi, Çukur Tuzak

  7. Review of Pharmacological Properties and Chemical Constituents of Pimpinella anisum.

    Science.gov (United States)

    Shojaii, Asie; Abdollahi Fard, Mehri

    2012-01-01

    Pimpinella anisum (anise), belonging to Umbelliferae family, is an aromatic plant which has been used In Iranian traditional medicine (especially its fruits) as carminative, aromatic, disinfectant, and galactagogue. Because the wide traditional usage of Pimpinella anisum for treatment of diseases, in this review published scientific reports about the composition and pharmacological properties of this plant were collected with electronic literature search of GoogleScholar, PubMed, Sciencedirect, Scopus, and SID from 1970 to 2011. So far, different studies were performed on aniseeds and various properties such as antimicrobial, antifungal, antiviral, antioxidant, muscle relaxant, analgesic and anticonvulsant activity as well as different effects on gastrointestinal system have been reported of aniseeds. It can also reduce morphine dependence and has beneficial effects on dysmenorrhea and menopausal hot flashes in women. In diabetic patients, aniseeds showed hypoglycemic and hypolipidemic effect and reduce lipid peroxidation. The most important compounds of aniseeds essential oil were trans-anetole, estragole, γ-hymachalen, para-anisaldehyde and methyl cavicol. Due to broad spectrum of pharmacological effects, and very few clinical studies of Pimpinella anisum, more clinical trials are recommended to evaluate the beneficial effects of this plant in human models and synthesis of new drugs from the active ingredients of this plant in future.

  8. Neuro beneficial effects of Pimpinella anisum against lead exposure

    Directory of Open Access Journals (Sweden)

    Khaled Kahloula

    2013-01-01

    Full Text Available Background: The essential oil of Pimpinella anisum has been widely used in traditional medicine to treat a variety of diseases, including some neurological disorders. Aims: This study was aimed to test, in vivo, the possible anxiolytic and antidepressant effects, of the essential oil of Pimpinella anisum against chronic lead acetate (0.2% intoxication during the gestation and lactation period, in Wistar rat pups. Settings and Design: Wistar rat pups were exposed to lead via their dams′ drinking water from postnatal day (PND 1 to (PND 21. After weaning, the lead-exposed rats received injections of essential oil of Pimpinella anisum (0.5 ml/kg for 15 days. The level of anxiety, depression and locomotor activity were studied. Materials and Methods: The behaviours evaluated were: Locomotor activity (open-field test, anxiety (dark and light compartment and elevated plus maze tests, and depression (forced swimming test. Statistical Analysis: The data were analysed by two-way analyses of variance (ANOVAs. When a significant difference was found, the Student-Newman-Keuls post-hoc test was conducted. For all analyses, the difference was considered to be significant at P ≤ 0.05. Results: The results of the present study demonstrate that developmental lead exposure induces, on the one hand, impairments of body ( P < 0.001 and brain weight ( P < 0.05, respectively, and on the other hand, increases the level of anxiety ( P < 0.001, depression ( P < 0.001 and locomotor hyperactivity ( P < 0.001, compared to control rats. Administration of the essential oil of Pimpinella anisum entrains reduction in the level of anxiety ( P < 0.001, depression ( P < 0.001 and correct locomotor hyperactivity ( P < 0.001 in rats exposed to lead beforehand. Conclusion: In conclusion, our results demonstrate that developmental lead exposure induces significant perturbation of emotional reactivity that can be improved by treatment with the essential oil of Pimpinella anisum

  9. Effect of aqueous and ethanolic extracts of Pimpinella anisum L. seeds on milk production in rats.

    Science.gov (United States)

    Hosseinzadeh, Hossein; Tafaghodi, Mohsen; Abedzadeh, Shirin; Taghiabadi, Elahe

    2014-08-01

    Pimpinella anisum L. (P. anisum) is used as a galactagogue in traditional medicine; hence, the effect of aqueous and ethanolic extracts of P. anisum seeds on milk production in rats was evaluated. The milk production was assessed by measuring the pups' weights during the suckling period. The intraperitoneal LD(50) values of P. anisum aqueous and ethanolic extracts were 4.93 and 3.77 g/kg, respectively. The aqueous (1 g/kg) and ethanolic extracts (1 g/kg) increased the milk production significantly (p anisum aqueous and ethanolic extracts can increase milk production in rats.

  10. Anticonvulsant and neuroprotective effects of Pimpinella anisum in rat brain

    Directory of Open Access Journals (Sweden)

    Karimzadeh Fariba

    2012-06-01

    Full Text Available Abstract Background Essential oil of Pimpinella anisum L. Apiaceae (anise oil has been widely used in traditional Persian medicine to treat a variety of diseases, including some neurological disorders. This study was aimed to test the possible anti-seizure and anti-hypoxia effects of anise oil. Methods The effects of different concentrations of anise oil were tested on seizure attacks induced by pentylenetetrazol (PTZ injection and neuronal hypoxia induced by oxygen withdrawal as well as on production of dark neurons and induction of long-term potentiation (LTP in in vivo and in vitro experimental models of rat brain. Results Anise oil significantly prolonged the latency of seizure attacks and reduced the amplitude and duration of epileptiform burst discharges induced by injection of intraperitoneal PTZ. In addition, anise oil significantly inhibited production of dark neurons in different regions of the brain in epileptic rats. Anise oil also significantly enhanced the duration of the appearance of anoxic terminal negativity induced by oxygen withdrawal and inhibited induction of LTP in hippocampal slices. Conclusions Our data indicate the anticonvulsant and neuroprotective effects of anise oil, likely via inhibition of synaptic plasticity. Further evaluation of anise oil to use in the treatment of neurological disorders is suggested.

  11. In vitro Antibacterial activity of Pimpinella anisum fruit extracts against some pathogenic bacteria

    Directory of Open Access Journals (Sweden)

    A.Akhtar

    Full Text Available The antibacterial activities of the aqueous, 50% (v/v methanol,acetone and petroleum ether extracts of Pimpinella anisum (L fruits were studied. The extracts of Pimpinella anisum were tested in vitro against 4 bacterial species by the disc diffusion method. Staphylococcus aureus (MTCC 96, Streptococcus pyogenes (MTCC 442, Escherchia coli (MTCC 723 and Klebsiella Pneumoniae (MTCC 109 were used in this investigation. Only aqueous and 50% (v/v methanol extract exhibited fair antibacterial activity against all the test bacteria whereas acetone and petroleum ether extract were not observed to inhibit the growth of any of the test bacteria under study. [Veterinary World 2008; 1(9.000: 272-274

  12. Synergistic antibacterial activity between Thymus vulgaris and Pimpinella anisum essential oils and methanol extracts.

    Science.gov (United States)

    Al-Bayati, Firas A

    2008-03-28

    Essential oils (EOs) and methanol extracts obtained from aerial parts of Thymus vulgaris and Pimpinella anisum seeds were evaluated for their single and combined antibacterial activities against nine Gram-positive and Gram-negative pathogenic bacteria: Staphylococcus aureus, Bacillus cereus, Escherichia coli, Proteus vulgaris, Proteus mirabilis, Salmonella typhi, Salmonella typhimurium, Klebsiella pneumoniae and Pseudomonas aeruginosa. The essential oils and methanol extracts revealed promising antibacterial activities against most pathogens using broth microdilution method. Maximum activity of Thymus vulgaris and Pimpinella anisum essential oils and methanol extracts (MIC 15.6 and 62.5mug/ml) were observed against Staphylococcus aureus, Bacillus cereus and Proteus vulgaris. Combinations of essential oils and methanol extracts showed an additive action against most tested pathogens especially Pseudomonas aeruginosa.

  13. The fruit essential oil of Pimpinella anisum exerts anticonvulsant effects in mice.

    Science.gov (United States)

    Pourgholami, M H; Majzoob, S; Javadi, M; Kamalinejad, M; Fanaee, G H; Sayyah, M

    1999-08-01

    This study investigates anticonvulsant effects of an essential oil of the fruits of Pimpinella anisum (Umbelliferae), a folkloric remedy in the Iranian traditional medicine, against seizures induced by pentylenetetrazole (PTZ) or maximal electroshock (MES) in male mice. The essential oil suppressed tonic convulsions induced by PTZ or MES. It also elevated the threshold of PTZ-induced clonic convulsions in mice. The essential oil produced motor impairment. However, this effect was not observed at the doses and time courses needed for anticonvulsant activity.

  14. Effects of Single and Combined Application of Organic and Biological Fertilizers on Quantitative and Qualitative Yield of Anisum (Pimpinella anisum

    Directory of Open Access Journals (Sweden)

    N Kamayestani

    2015-07-01

    Full Text Available In order to study the effects of single and combined applications of biofertilazer and organic fertilizers on quantitative and qualitative characteristics of anisum (Pimpinella anisum, an experiment was conducted based on a Randomized Complete Block Design with three replications and fifteen treatments at Research Station, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran, in 2011 year. Treatments were: (1 mycorrhiza (Glomus intraradices, (2 mycorrhiza + cow manure, (3 mycorrhiza + vermicompost, (4 mycorrhiza+ compost, (5 mycorrhiza + chemical fertilizer, (6 biosulfur (Thiobacillus sp. + Bentonite, (7 biosulfur + chemical fertilizer, (8 biosulfur + cow manure, (9 biosulfur + vermicompost, (10 biosulfur+compost,11 (cow manure, (12 vermicompost, (13 chemical fertilizer (NPK, (14compost and (15 control. The results showed that application of fertilizer treatments had significant effect on most characteristics of anisum. The highest number of seed per umbelet (7.24, economic yield (1263.4kg/ha were obtained fram biosulfur treatment. The highest dry matter yield (4504.1 kg/ha resulted from combined application of biosulfur + chemical fertilizer and the highest harvest index (25.97% observed in biosulfur+cow manure. The combined application of mycorrhiza affected some qualification traits, as the highest number of umbel per plant (65.7, 1000 seed-weight (3.24 g and essential oil percentage (5.3% resulted from combined application of mycorrhiza+chemical fertilizer. In general, it can be concluded that application of organic and biological fertilizer particularly mycorrhiza and biosulfur had a significant effect on improving of quantitative and qualitative characteristics of anisum. Furthermore, the combined application of organic and biological fertilizer had higher positive effects than their single application.

  15. Green Synthesis of Silver Nanoparticles Using Pimpinella anisum L. Seed Aqueous Extract and Its Antioxidant Activity

    Directory of Open Access Journals (Sweden)

    Hashem Akhlaghi

    2015-09-01

    Full Text Available An aqueous extract of Pimpinella anisum was used for green synthesis of silver nanoparticles by bio reduction of an aqueous solution of silver nitrate. Silver nanoparticles were characterized by UV–Vis spectrometry, Fourier transform infrared spectroscopy (FTIR, X-ray diffraction (XRD analysis, scanning electron microscopy (SEM and energy-dispersive X-ray analysis (EDAX. The increase in absorption at 420 nm was used for recording the formation of a colloidal suspension of silver nanoparticles. The binding properties of the capped Ag nanoparticles synthesized from aqueous extract of P. anisum were analyzed by FTIR. XRD studies revealed that most of the nanoparticles were cubic and face centered cubic in shape. SEM analysis showed the size and shape of silver nanoparticles and EDAX confirmed the presence of silver. The synthesized silver nanoparticles showed DPPH free radical scavenging activity.

  16. Antiviral and immunostimulating effects of lignin-carbohydrate-protein complexes from Pimpinella anisum.

    Science.gov (United States)

    Lee, Jung-Bum; Yamagishi, Chihiro; Hayashi, Kyoko; Hayashi, Toshimitsu

    2011-01-01

    Three antiviral and immunostimulating substances (LC1, LC2 and LC3) were isolated from a hot water extract of seeds of Pimpinella anisum by combination of anion-exchange, gel filtration and hydrophobic interaction column chromatographies. Chemical and spectroscopic analyses revealed them to be lignin-carbohydrate-protein complexes. These lignin-carbohydrate complexes (LCs) showed antiviral activities against herpes simplex virus types 1 and 2 (HSV-1 and -2), human cytomegalovirus (HCMV) and measles virus. LCs were also found to interfere with virus adsorption to the host cell surface and directly inactivate viruses. Furthermore, they enhanced nitric oxide (NO) production by inducing iNOS mRNA and protein expression in RAW 264.7 murine macrophage cells. The induced mRNA expression of cytokines including IL-1β and IL-10 was also apparent. These results suggest that the lignin-carbohydrate-protein complexes from P. anisum possessed potency as functional food ingredients against infectious diseases.

  17. Extraction of essential oil from Pimpinella anisum using supercritical carbon dioxide and comparison with hydrodistillation.

    Science.gov (United States)

    Yamini, Yadollah; Bahramifar, Nader; Sefidkon, Fatemeh; Saharkhiz, Mohamad Jamal; Salamifar, Ehsan

    2008-02-15

    Supercritical fluid extraction (SFE) of essential oil from Pimpinella anisum, using carbon dioxide as a solvent is presented in this work. An orthogonal array design OA9 (3(4)) was applied to select the optimum extraction condition. The effects of pressure, temperature, dynamic extraction time and methanol volume on the extraction efficiency were investigated by the three-level orthogonal array design. Results show that pressure has a significant effect on the extraction efficiency. The extract obtained from P. anisum by using supercritical fluid extraction was compared with the essential oil obtained by hydrodistillation, considering both quantity and quality of the product. SFE products were found to be of markedly different composition, compared with the corresponding hydrodistilated oil. The total amount of extractable substances obtained in SFE (7.5%) is higher than that obtained by hydrodistillation (3.1%) and SFE is faster than hydrodistillation method.

  18. IMPACT OF ANISE (PIMPINELLA ANISUM AND MINT (MENTHA PIPERITA ESSENTIAL OILS TO MICROBIAL ACTIVITY IN CHICKEN MEAT

    Directory of Open Access Journals (Sweden)

    Miroslava Kačániová

    2015-02-01

    Full Text Available The goal of the present study was to evaluate fresh chicken thighs quality (microbiological and sensory after treatment by ethylenediaminetetraacetate (EDTA, Pimpinella anisum L. and Mentha piperita essential oils in 1% concentration, stored under vacuum packaging (VP, at 4±0.5°C for a period of 16 days. The following treatments of chicken thighs were applied: air-packaged (AC, control samples, vacuum-packaged (VPC, control samples, vacuum-packed with EDTA solution 1.50% v/w (VPEC, control samples, VP with Pimpinella anisum L. and Mentha piperita essential oil at concentrations 0.1% v/w (VP+AEO and VP+MEO. The quality assessment of VP product after EDTA treatment, Pimpinella anisum L. and Mentha piperita oils was done by microbiological testing and the total viable counts, Enterobacteriaceae, lactic acid bacteria and Pseudomonas aeruginosa were detected. The using of Pimpinella anisum L. and Mentha piperita oils and EDTA with combination of vacuum packaging has significant effect (P < 0.05 to reduction of microorganisms compared with control group without vacuum packaging and untreated control group.

  19. Isolation and identification of a new, naturally occurring cytokinin (6-benzylaminopurineriboside) from an anise cell culture (Pimpinella anisum L.).

    Science.gov (United States)

    Ernst, D; Schäfer, W; Oesterhelt, D

    1983-11-01

    A new, naturally occurring cytokinin was isolated from a cell culture of anise (Pimpinella anisum L.). Its structure was determined as 6-benzylaminopurine riboside by the use of gas chromatography and mass spectrometry. Quantitation of this new plant hormone was accomplished by single-ion-monitoring measurements.

  20. The Study on the Effects of Pimpinella anisum on Relief and Recurrence of Menopausal Hot Flashes.

    Science.gov (United States)

    Nahidi, Fatemeh; Kariman, Nourossadat; Simbar, Masoumeh; Mojab, Faraz

    2012-01-01

    With respect to the high incidence of hot flash in postmenopausal women and the controversies regarding its treatment, this double-blind clinical trial was conducted to determine the effects of Pimpinella anisum on hot flashes in these women referring to rural and urban health centers of Qazvin Province in 2009. Seventy-two women with hot flashes were randomly selected according to the predetermined criteria and divided into two experimental and control groups. Their medical records at health centers were used for sampling. Each woman in the experimental group took a capsule containing 330 mg Pimpinella anisum 3 times a day while in the control group, women took 3 capsules, each containing 330 mg potato starch, over 4 weeks and after that, they were following up for 4 weeks. Before taking the capsules, they were assessed for 2 weeks about the frequency and severity of hot flashes. Data were collected through a questionnaire and an information form. Content validity method was used for validity of the tools. ANOVA and Student›s t-test were applied for statistical analysis. In the experimental group, the frequency and severity of hot flashes before the treatment were 4.21% and 56.21% and, after that, were 1.06% and 14.44% at the end of the fourth week respectively. No change was found in the frequency and severity of hot flashes in the control group. The frequency and severity of hot flashes was decreased during 4 weeks of follow up period. P. anisum is effective on the frequency and severity of hot flashes in postmenopausal women.

  1. STANDARDIZATION AND TOXICOLOGICAL EVALUATION FOR EFFICACY AND SAFETY OF PIMPINELLA ANISUM L.

    Directory of Open Access Journals (Sweden)

    Kalam N

    2012-07-01

    Full Text Available The demand of herbal medicine is increasing day by day due to their efficacy, safety (rare chances of side effects in the treatment and good faith of society on herbal medicine and also their products. Therefore standardization and toxicological study of herbal medicine is quite necessary to check quality, Purity, strength and safety of the drugs. The present work is an attempt to evaluate toxicological parameters for safety of an herbal drug Pimpinella anisum L. such as microbial contamination, aflatoxins, pesticide residues and heavy metals. Additional standardization parameters like, physical constants, ash content, solvent residues, and microscopical analysis were also carried out and reported. The results obtained may be helpful to the regulatory authorities, scientific organizations and manufacturers for developing standards and maintaining the quality of drugs.

  2. Isolation of terpenoids from Pimpinella anisum essential oil by high-performance counter-current chromatography.

    Science.gov (United States)

    Skalicka-Woźniak, Krystyna; Walasek, Magdalena; Ludwiczuk, Agnieszka; Głowniak, Kazimierz

    2013-08-01

    High-performance counter-current chromatography was successfully used for the isolation and purification of terpenoid compounds from the essential oil of Pimpinella anisum L. A two-phase solvent system composed of n-heptane/methanol/ethyl acetate/water (5:2:5:2, v/v/v/v) was suitable for the purification of linalool, terpinen-4-ol, α-terpineol, p-anisaldehyde, while n-heptane/methanol (1:1, v/v) was used for the isolation of anethole and foeniculin. A scale-up process from analytical to preparative was developed. Additionally, a stepwise gradient elution was applied and instead of two different runs, 40 min each, one 80 min separation was performed; although the time of separation remains the same, it was possible to repeat the efficiency even if the water-containing mobile phase was changed to a nonaqueous system. The obtained essential oil, as well as purified compounds, was analyzed by GC. A total of 0.64 mg of linalool, 0.52 mg of terpinen-4-ol, 0.10 mg of α-terpineol, 0.62 mg of p-anisaldehyde, 15 mg of anethole, and 2.12 mg of foeniculin were obtained from 210 mg of the essential oil of P. anisum L. in a short time with purities of 99, 98, 94, 93.54, 93, and 93.6%, respectively.

  3. In vitro evaluation of antibacterial activity of hydroalcoholic extract of Salvia officinalis and Pimpinella anisum against cariogenic bacteria

    Directory of Open Access Journals (Sweden)

    Kermanshah H.

    2009-11-01

    Full Text Available "nBackground and Aim: The microbial cause of dental caries has been proved among its multifactorial etiologies. The prevention and control of dental caries is very critical because of high prevalence and cost especially in high risk patient such as xerostomia. Based on the world attraction to traditional treatment and importance of drug extraction of natural materials and plants, in this in vitro study effect of hydroalcoholic extract of Salvia officinalis and Pimpinella anisum which were very useful in traditional treatment of mouth and teeth disease has been determined against cariogenic bacteria. "nMaterials and Methods: In this experimental study, hydroalcoholic extracts have been prepared from Salvia officinalis and Pimpinella anisum with maceration method. Their antibacterial activity against Streptococcus mutans, Lactobacillus rhamnosus and Actinomyces viscosus have been evaluated with broth macrodilution method. Data were analyzed with Mann-Whitney test. "nResults: Minimum Inhibitory Concentration for Salvia officinalis and Pimpinella anisum for streptococcus mutans were respectively 6.25 and 12.5 µg/ml, for Lactobacillus rhamnosus were 1.56 and 12.5 µg/ml and for Actinomyces viscosus were 12.5 and 50 µg/ml. "nConclusion:Both extracts had growth inhibitory effect on all three bacteria. Salvia officinalis had significantly greater effect on inhibition of growth of all three bacteria. Both extracts had bactericidal effect in the range of concentration.

  4. Presença de matérias estranhas em erva-doce, Pimpinella anisum L. | Presence of foreign matter in anise, Pimpinella anisum L.

    Directory of Open Access Journals (Sweden)

    Juliana Machado Santos

    2015-11-01

    Full Text Available A palavra “chá” é usada popularmente no Brasil como sinônimo de infusão de frutos, folhas, raízes ou ervas como camomila, hortelã, erva-doce, capim-cidreira. A Resolução da Diretoria Colegiada RDC nº 277/2005 define “chá” como “o produto constituído de uma ou mais partes de espécie(s vegetal(is inteira(s, fragmentada(s ou moída(s, com ou sem fermentação, tostada(s ou não”. O produto deve ser designado como “chá”, seguido do nome comum da espécie vegetal utilizada. O Codex Alimentarius considera crianças e idosos grupos de risco quanto à presença de matérias estranhas em alimentos. O presente estudo teve como objetivo avaliar a qualidade, através da pesquisa de matérias estranhas, de dez marcas de “chás” da espécie vegetal Pimpinella anisum L. oferecidas no mercado varejista do Rio de Janeiro. Foram utilizadas as metodologias para alimentos da Association of Official Analytical Chemists de 2010 e para droga vegetal da Farmacopeia Brasileira de 2010. Das onze marcas, seis apresentaram presença de matérias estranhas acima de 2%, conforme estabelecido na Farmacopeia Brasileira. Em todas as amostras foram detectadas a presença de terra e areia indicativas de falhas das Boas Práticas pelas legislações específicas. =============================================== The word “tea” is popularly used in Brazil as a synonym for the infusion of fruits, leaves, roots, or herbs, such as chamomile, mint, fennel, and lemongrass. The Collegiate Board Resolution (RDC No. 277/2005 defines “tea” as a product consisting of one or more parts of plant species, whole, fragmented, or ground, with or without fermentation, and toasted or untoasted. The product must be designated as “tea,” followed by the common name of the plant species used. The Codex Alimentarius defines children and the elderly as risk groups for the presence of foreign matter in food. This study aims to evaluate the quality of tea offered

  5. Ameliorative effect of Pimpinella anisum oil on immunohistochemical and ultrastuctural changes of cerebellum of albino rats induced by aspartame.

    Science.gov (United States)

    Abdul-Hamid, Manal; Gallaly, Sanaa Rida

    2014-05-01

    The study aims to investigate the protective effect of Pimpinella anisum oil on aspartame (ASP) which resulted in cerebellar changes. The rats were divided into four equal groups: Group 1: (control group): served as control animals. Group 2: control P. anisum oil received .5 mL/kg/d/b wt. once daily. Group 3 (ASP group): received daily 250 mg/kg/b wt. of ASP dissolved in distilled water and given orally to the animals by intra-gastric tube for 2 months. Group 4: received .5 mL/kg/b wt. of prophylactic P. anisum oil once daily, followed by ASP after 2 h for 2 months. The histopathological approach revealed marked changes in the Purkinje cells, myleinated nerve fibers and granular cells of ASP-treated animals. Some of these cells appeared with deeply stained cytoplasm. Ultrastructural examination showed Purkinje cells with dilated rough endoplasmic reticulum and condensed mitochondria. Granular cells appeared with less c nuclei and surrounded by dissolution of most Mossy rosettes structures. Most myelinated nerve fibers showed thickening of myelinated sheath and others showed splitting of their myelin sheath. The histopathological, immunohistochemical and ultrastructural alterations were much less observed in concomitant use of P. anisum oil with ASP. Cerebellar cortex is considered target areas of ASP neurotoxicity, while P. anisum oil, when used in combination with ASP displays a protective action against neurotoxicity.

  6. 药用植物茴芹及其病害防治措施探讨%Discussion on Medicinal Plant Pimpinella anisum L. and Diseases Prevention

    Institute of Scientific and Technical Information of China (English)

    穆合塔尔·麦提图尔荪; 凯麦尔妮萨·艾合麦提托合提; 严军

    2016-01-01

    Pimpinella anisum L. was not only medicine,but also condiment. Starting from introducing the morpholog-ical characteristics of Pimpinella anisum L.,the cultivation techniques were elab-orated. The medicinal values of Pimpinella anisum L. were discussed. Moreover,the main disease and its con-trol methods were put forward.%茴芹既是中药材又是调味品。从介绍茴芹形态特征出发,阐述了其栽培技术,论述了茴芹药用价值,并详细介绍了主要病害及其防治方法。

  7. Aqueous suspension of anise "Pimpinella anisum" protects rats against chemically induced gastric ulcers

    Institute of Scientific and Technical Information of China (English)

    Ibrahim A Al Mofleh; Abdulqader A Alhaider; Jaber S Mossa; Mohammed O Al-Soohaibani; Syed Rafatullah

    2007-01-01

    AIM:To substantiate the claims of Unani and Arabian traditional medicine practitioners on the gastroprotective potential effect of a popular spice anise,"Pimpinella anisum L." on experimentally-induced gastric ulceration and secretion in rats.METHODS:Acute gastric ulceration in rats was produced by various noxious chemicals including 80% ethanol,0.2 mol/L NaOH,25% NaCl and indomethacin.Anti-secretory studies were undertaken using pylorusligated Shay rat technique.Levels of gastric non-protein sulfhydryls(NP-SH)and wall mucus were estimated and gastric tissue was also examined histologically.Anise aqueous suspension was used in two doses(250 and 500 mg/kg body weight)in all experiments.RESULTS:Anise significantly inhibited gastric mucosal damage induced by necrotizing agents and indomethacin.The anti-ulcer effect was further confirmed histologically.In pylorus-ligated Shay rats,anise suspension significantly reduced the basal gastric acid secretion,acidity and completely inhibited the rumenal ulceration.On the other hand,the suspension significantly replenished ethanol-induced depleted levels of gastric mucosal NP-SH and gastric wall mucus concentration.CONCLUSION:Anise aqueous suspension possesses significant cytoprotective and anti-ulcer activities against experimentally-induced gastric lesions.The anti-ulcer effect of anise is possibly prostaglandin-mediated and/or through its anti-secretory and antioxidative properties.

  8. Essential oil composition of Pimpinella anisum L. fruits from various European countries.

    Science.gov (United States)

    Orav, Anne; Raal, Ain; Arak, Elmar

    2008-02-15

    Variations in the essential oil composition of Pimpinella anisum L. fruits obtained from different geographical areas of Europe were determined using capillary GC and GC-MS techniques. The essential oil content of the samples was 10.0-53.6 mL kg(-1) and did not confirm to the European Pharmacopoeia standard in 5 samples out of 14. A total of 21 compounds were identified and significant quantitative differences were observed among the samples. The major component was trans-anethole (76.9-93.7%); the other principal compounds in oils were gamma-himachalene (0.4-8.2%), trans-pseudoisoeugenyl 2-methylbutyrate (0.4-6.4%), p-anisaldehyde (tr-5.4%) and methylchavicol (0.5-2.3%). The highest content of trans-anethole (>90%) was found in the samples from Greece, Hungary, Scotland, Lithuania, Italy, and Germany (2 samples). Essential oil of aniseed from Estonia was rich in gamma-himachalene (8.2%) and trans-pseudoisoeugenyl 2-methylbutyrate (6.4%). The sample from France contained the highest amount of anisaldehyde (5.4%) comparing with other samples (0-3.1%). beta-Bourbonene and alpha-farnesene are determined in anise oil for the first time.

  9. Antifungal activity of fluid extract and essential oil from anise fruits (Pimpinella anisum L., Apiaceae).

    Science.gov (United States)

    Kosalec, Ivan; Pepeljnjak, Stjepan; Kustrak, Danica

    2005-12-01

    Antifungal activities of fluid extract and essential oil obtained from anise fruits Pimpinella anisum L. (Apiaceae) were tested in vitro on clinical isolates of seven species of yeasts and four species of dermatophytes. Diffusion method with cylinders and the broth dilution method were used for antifungal activity testing. Anise fluid extract showed antimycotic activity against Candida albicans, C. parapsilosis, C. tropicalis, C. pseudotropicalis and C. krusei with MIC values between 17 and 20% (v/v). No activity was noticed against C. glabrata, and anis fruits extracts showed growth promotion activity on Geotrichum spp. Anise fruits extract inhibited the growth of dermatophyte species (Trichophyton rubrum, T. mentagrophytes, Microsporum canis and M. gypseum) with MIC values between 1.5 and 9.0% (V/V). Anise essential oil showed strong antifungal activity against yeasts with MIC lower than 1.56% (V/V) and dermatophytes with MIC lower than 0.78% (V/V). Significant differences in antifungal activities were found between anise fluid extract and anise essential oil (p<0.01). Anise essential oil exhibited stronger antifungal activities against yeasts and dermatophytes with MIC values between 0.10 and 1.56% (V/V), respectively.

  10. Effect of gamma irradiation on microbial load and sensory characteristics of aniseed (Pimpinella anisum).

    Science.gov (United States)

    Al-Bachir, M

    2007-07-01

    Seeds of anise (Pimpinella anisum) were exposed to doses of 0, 5, 10, 15 and 20kGy in a (60)Co package irradiator. Irradiated and unirradiated samples were stored at room temperature. Microbial populations on seeds, total and inorganic soluble solids in water extract and sensory properties of the latter were evaluated after 0, 6 and 12 months of storage. Results indicated that gamma irradiation reduced the aerobic plate counts of aniseed. Immediately after irradiation, the total soluble solids in an extract of irradiated seeds were greater than those of unirradiated ones. The total soluble solids in an extract of irradiated and un-irradiated seeds increased after 6 and 12 months of storage. There were no significant differences (p>0.05) in inorganic soluble solids between the water extract of irradiated and unirradiated aniseeds. Sensory evaluation indicated that gamma irradiation improved sensory characteristics of aniseed water extract tested immediately after irradiation; however, after 12 months of storage, no significant differences (p>0.05) were found in color, taste or flavor between extract of irradiated and unirradiated seeds.

  11. Loss of competence for glyoxysome formation during somatic embryogenesis in anise (Pimpinella anisum L.) suspension cultures.

    Science.gov (United States)

    Kudielka, R A; Theimer, R R

    1983-10-01

    Somatic embryogenesis in anise (Pimpinella anisum L.) suspension cultures induced by transfer to hormone-free growth medium may be synchronized by previous selection of cell aggregates with diameters between 100-240 μm. Around 80-90% of the embryoids are globular after 2-3 d, heart-shaped after 5-7 d and torpedo-shaped after 9 d. In embryogenic medium without source of carbon or with 20 mmol/l acetate differentiation and growth cease. But like in dedifferentiated cell aggregates the key enzyme activities for glyoxysomes such as isocitrate lyase and malate synthase are induced in globular (3 d old) and heart-shaped (5 d old) embryoids, but not in embryoids at day 7 or later. Similarly, in explants from anise hypocotyl glyoxysomes cannot be derepressed by such treatment. It is concluded that during differentiation of heart-shaped embryoids to torpedo forms the competence of the cells for the yet unknown inducing principle for glyoxysomes is lost.

  12. IMPACT OF ANISE (PIMPINELLA ANISUM) AND MINT (MENTHA PIPERITA) ESSENTIAL OILS TO MICROBIAL ACTIVITY IN CHICKEN MEAT

    OpenAIRE

    2015-01-01

    The goal of the present study was to evaluate fresh chicken thighs quality (microbiological and sensory) after treatment by ethylenediaminetetraacetate (EDTA), Pimpinella anisum L. and Mentha piperita essential oils in 1% concentration, stored under vacuum packaging (VP), at 4±0.5°C for a period of 16 days. The following treatments of chicken thighs were applied: air-packaged (AC, control samples), vacuum-packaged (VPC, control samples), vacuum-packed with EDTA solution 1.50% v/w (VPEC, contr...

  13. Antispasmodic and relaxant effects of the hidroalcoholic extract of Pimpinella anisum (Apiaceae) on rat anococcygeus smooth muscle.

    Science.gov (United States)

    Tirapelli, Carlos R; de Andrade, Claudia R; Cassano, Adriano O; De Souza, Fernando A; Ambrosio, Sergio R; da Costa, Fernando B; de Oliveira, Ana M

    2007-03-01

    The present work describes the mechanisms involved in the muscle relaxant effect of ethanol:water (40:60, 60:40 and 80:20) aerial parts extracts of Pimpinella anisum. Three hidroalcoholic extracts in which the proportion of ethanol was 40% (HA(40%)), 60% (HA(60%)) or 80% (HA(80%)) were tested for activity in the rat anococcygeus smooth muscle. The three extracts (50 microg/mL) inhibited acetylcholine-induced contraction. The extract HA(60%) (5-50 microg/mL) concentration dependently relaxed acetylcholine-pre-contracted tissues (31.55+/-3.56%). Conversely, HA(40%) and HA(80%) did not exert relaxant action. Pre-incubation of the preparations with N(G)-nitro-L-arginine methyl ester (L-NAME, 100 microM), 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ, 3 microM) and oxyhemoglobin (10 microM) reduced the relaxation induced by HA(60%) (percentage of relaxation: 6.81+/-1.86%, 13.13+/-5.87% and 2.12+/-1.46%, respectively). Neither indomethacin (10 microM) nor tetraethylammonium (1 mM) affected the relaxation induced by HA(60%). Incubation of the tissues with L-NAME significantly enhanced the maximal contraction induced by acetylcholine, indicating an inhibitory role for NO in the modulation of the contractile response of anococcygeus smooth muscle to acetylcholine. However, simultaneous addition of L-NAME and HA(60%) resulted in an effect similar to that observed with L-NAME alone, further confirming the observation that Pimpinella anisum acts by realizing NO. Additionally, HA(60%) did not alter CaCl(2)-induced contraction. Collectively, our results provide functional evidence that the effects elicited by the hidroalcoholic extract of Pimpinella anisum involve the participation of NO and subsequent activation of the NO-cGMP pathway. The relaxant action displayed by Pimpinella anisum justifies its use in the folk medicine as an antispasmodic agent.

  14. Pimpinella anisum in the treatment of functional dyspepsia: A double-blind, randomized clinical trial

    Directory of Open Access Journals (Sweden)

    S Ashraffodin Ghoshegir

    2015-01-01

    Full Text Available Background: We aimed to evaluate the effects of Pimpinella anisum (anise from Apiaceae family on relieving the symptoms of postprandial distress syndrome (PDS in this double-blind randomized clinical trial. Materials and Methods: Totally, 107 patients attending the gastroenterology clinic, aged 18-65 years, diagnosed with PDS according to ROME III criteria and signed a written consent form were enrolled. They were randomized to receive either anise or placebo, blindly, for 4 weeks. Anise group included 47 patients and received anise powders, 3 g after each meal (3 times/day. Control group involved 60 patients and received placebo powders (corn starch, 3 gafter each meal (3 times/day. The severity of Functional dyspepsia (FD symptoms was assessed by FD severity scale. Assessments were done at baseline and by the end of weeks 2, 4 and 12. Mean scores of severity of FD symptoms and the frequency distribution of patients across the study period were compared. Results: The age, sex, body mass index, smoking history, and coffee drinking pattern of the intervention and control groups were not significantly different. Mean (standard deviation total scores of FD severity scale before intervention in the anise and control groups were 10.6 (4.1 and 10.96 (4.1, respectively (P = 0.6. They were 7.04 (4.1 and 12.30 (4.3 by week 2, respectively (P = 0.0001, 2.44 (4.2 and 13.05 (5.2 by week 4, respectively (P = 0.0001, and 1.08 (3.8 and 13.30 (6.2 by week 12, respectively (P = 0.0001. Conclusion: This study showed the effectiveness of anise in relieving the symptoms of postpartum depression. The findings were consistent across the study period at weeks 2, 4 and 12.

  15. Influence of Ecological Conditions on Seeds Traits and Essential Oil Contents in Anise (Pimpinella anisum L.

    Directory of Open Access Journals (Sweden)

    Milica G ACIMOVIC

    2014-06-01

    Full Text Available Anise (Pimpinella anisum L. is an annual plant of the Apiaceae family, widely cultivated for seeds (fruits and essential oil. Under field condition anise is cultivated wide range of countries in Southern Europe including Serbia. Field experiments were carried out during two growing seasons, at three localities in order to determine the effect of different soil and climatic conditions on quality traits of anise seed principal traits (thousand seed mass, germination energy and total germination as well as on content and quality of its essential oil. During experiment thousand seed mass was significantly lower in 2012 in comparison to the previous year. Germination energy and total germination were significantly higher in 2011, because of more favourable weather conditions for seed development during this year. A significantly higher concentration of essential oil was accumulated in 2011 in comparison to 2012. This can be attributed to a longer period of fruit formation and synthesis of essential oils and better climate conditions. Unlike the content of essential oil, trans-anethole content was significantly higher in dry 2012 than in 2011. It can be concluded that drought caused significantly decrease in weight of thousand seed, germination energy and total germination and content of essential oil in anise. Contrary to this, trans-anethole content was significantly higher in the dry year. It can be assumed that under stress conditions the amount of trans-anethole in the essential oil increased, because in such conditions the plants produce more secondary metabolites, the substances that prevent oxidation processes in the cells.

  16. Pimpinella anisum in the treatment of functional dyspepsia: A double-blind, randomized clinical trial

    Science.gov (United States)

    Ghoshegir, S. Ashraffodin; Mazaheri, Mohammad; Ghannadi, Alireza; Feizi, Awat; Babaeian, Mahmoud; Tanhaee, Maryam; Karimi, Mehrdad; Adibi, Peyman

    2015-01-01

    Background: We aimed to evaluate the effects of Pimpinella anisum (anise) from Apiaceae family on relieving the symptoms of postprandial distress syndrome (PDS) in this double-blind randomized clinical trial. Materials and Methods: Totally, 107 patients attending the gastroenterology clinic, aged 18-65 years, diagnosed with PDS according to ROME III criteria and signed a written consent form were enrolled. They were randomized to receive either anise or placebo, blindly, for 4 weeks. Anise group included 47 patients and received anise powders, 3 g after each meal (3 times/day). Control group involved 60 patients and received placebo powders (corn starch), 3 gafter each meal (3 times/day). The severity of Functional dyspepsia (FD) symptoms was assessed by FD severity scale. Assessments were done at baseline and by the end of weeks 2, 4 and 12. Mean scores of severity of FD symptoms and the frequency distribution of patients across the study period were compared. Results: The age, sex, body mass index, smoking history, and coffee drinking pattern of the intervention and control groups were not significantly different. Mean (standard deviation) total scores of FD severity scale before intervention in the anise and control groups were 10.6 (4.1) and 10.96 (4.1), respectively (P = 0.6). They were 7.04 (4.1) and 12.30 (4.3) by week 2, respectively (P = 0.0001), 2.44 (4.2) and 13.05 (5.2) by week 4, respectively (P = 0.0001), and 1.08 (3.8) and 13.30 (6.2) by week 12, respectively (P = 0.0001). Conclusion: This study showed the effectiveness of anise in relieving the symptoms of postpartum depression. The findings were consistent across the study period at weeks 2, 4 and 12. PMID:25767516

  17. CHEMICAL COMPOSITION AND ANTIBACTERIAL PROPERTIES OF ESSENTIAL OILS OF Pimpinella Anisum L. GROWING IN MOROCCO AND YEMEN

    Directory of Open Access Journals (Sweden)

    A. AL MAOFARI

    2014-01-01

    Full Text Available We report in this study the chemical composition and antibacterial activities of the seed’s essential oils of Pimpinella anisum L. collected from Morocco and Yemen. The hydro-distillation technique was used to extract their essential oils, followed by continuous liquid-liquid fractionation using water and ethyl acetate as solvent system. Obtained essential oils were analyzed by gas chromatography (GC and gas chromatography-mass spectroscopy (GC-MS. GC and GC/MS results showed that 4-allylanisole was the major compound of Pimpinella anisum L. with percentages of 76.70 and 85.28% of Moroccan and Yemen, respectively, in addition to other minor compounds such as limonene (9.75% for Moroccan species and 5.53% for Yemen species and fenchone (6.16% for Moroccan species and 4.12% for Yemen species. Furthermore, both essential oils were evaluated for their antibacterial activity against a panel of pathogenic microorganisms. The results showed that both essential oils inhibit most pathogenic bacteria tested.

  18. Biosynthesis of pseudoisoeugenols in tissue cultures of Pimpinella anisum. Phenylalanine ammonia lyase and cinnamic acid 4-hydroxylase activities.

    Science.gov (United States)

    Reichling, J; Kemmerer, B; Sauer-Gürth, H

    1995-07-28

    The genus Pimpinella contains pseudoisoeugenols, phenylpropanoids with a rare 2,5-dioxy substitution pattern on the phenyl ring. To study the biosynthesis of these compounds, we set up a leaf-differentiating tissue culture of Pimpinella anisum. These cultures mainly produce epoxy-pseudoisoeugenol-(2-methylbutyrate). To corroborate the biosynthetic pathway of epoxy-pseudoisoeugenol-(2-methylbutyrate) as proposed on the basis of investigations with 13C/14C-labelled precursors, the key steps of the pathway were investigated at an enzyme level. Experiments with cell-free homogenates clearly revealed that L-phenylalanine is converted to (E)-cinnamic acid by phenylalanine ammonia lyase and that (E)-cinnamic acid is converted to p-coumaric acid by cinnamic acid 4-hydroxylase. L-2-aminooxy-3-phenylpropionic acid, an analogue of L-phenylalanine, inhibited the incorporation of L-[3'-13C]phenylalanine into epoxy-pseudoisoeugenol-(2-methylbutyrate). Up to 2% of the precursor DL-[3'-13C]phenyllactate was incorporated into epoxy-pseudoisoeugenol-(2-methylbutyrate). Inhibition experiments with oxalacetic acid clearly showed that cinnamic acid is not formed by dehydration of phenyllactic acid in this leaf-differentiating tissue culture of P. anisum.

  19. Resolution and quantification of isomeric fatty acids by silver ion HPLC: fatty acid composition of aniseed oil (Pimpinella anisum, Apiaceae).

    Science.gov (United States)

    Denev, Roumen V; Kuzmanova, Ivalina S; Momchilova, Svetlana M; Nikolova-Damyanova, Boryana M

    2011-01-01

    A silver ion HPLC procedure is described that is suitable to determine the fatty acid composition of plant seed oils. After conversion of fatty acids to p-methoxyphenacyl derivatives, it was possible to achieve baseline resolution of all fatty acid components with 0 to 3 double bonds, including the positionally isomeric 18:1 fatty acids oleic acid (cis 9-18:1), petroselinic acid (cis 6-18:1), and cis-vaccenic acid (cis 11-18:1), in aniseed oil (Pimpinella anisum, Apiaceae) by a single gradient run on a single cation exchange column laboratory converted to the silver ion form. The UV detector response (280 nm) was linearly related to the fatty acid concentration in the range 0.01 to 3.5 mg/mL.

  20. The effects of fruit essential oil of the Pimpinella anisum on acquisition and expression of morphine induced conditioned place preference in mice.

    Science.gov (United States)

    Sahraei, Hedayat; Ghoshooni, Hassan; Hossein Salimi, Sayed; Mohseni Astani, Abutaleb; Shafaghi, Bijan; Falahi, Mansoor; Kamalnegad, Mohammad

    2002-04-01

    The problem of drug dependence still remains unresolved. In the present study the effects of an essential oil of Pimpinella anisum (Umbeliferae) on the expression and acquisition of conditioned place preference (CPP) induced by morphine in mice were investigated. Subcutaneous (s.c.) injections of morphine (2-5 mg/kg) produced place preference in a dose-dependent manner. Furthermore, intraperitoneal (i.p.) injection of the essential oil of P. anisum (0.125-0.5 ml/kg) induced conditioned place aversion (CPA). The mice which have received the essential oil of the P. anisum (0.125-0.5 ml/kg, i.p.) as well as the oil with morphine (5 mg/kg, s.c.) reduced the morphine effect. Administration of the essential oil of P. anisum (0.125-0.5 ml/kg, i.p.) on the test day did not show any effect on morphine action. It appeared that pre-administration with bicuculline (GABA(A) receptor antagonist) (1.5 mg/kg, i.p., 20 min before essential oil) diminished the effect of the essential oil of the P. anisum on morphine which induced CPP, but this result was not found for the GABA(B) receptor antagonist, CGP35348 (200 and 400 mg/kg, i.p., 10 min before essential oil). In conclusion, it appeared that the essential oil of the P. anisum may reduce the morphine effects via a GABAergic mechanism.

  1. Relaxant effect of Pimpinella anisum on isolated guinea pig tracheal chains and its possible mechanism(s).

    Science.gov (United States)

    Boskabady, M H; Ramazani-Assari, M

    2001-01-01

    We have studied the relaxant effect of Pimpinella anisum on isolated guinea pig tracheal chains and its possible mechanism(s). The bronchodilatory effects of aqueous and ethanol extracts and essential oil were examined on precontracted isolated tracheal chains of the guinea pig by 10 microM methacholine in two different conditions including: non-incubated tissues (group 1) and incubated tissues with 1 microM propranolol and 1 microM chlorpheniramine (group 2). In addition, the anticholinergic effects of essential oil and 10 nM atropine were tested by comparing the cumulative log concentration-response curves of methacholine induced contraction of tracheal chains and the effective concentration of methacholine, causing 50% of maximum response (EC(50)) in the presence of essential oil or atropine. Aqueous and ethanol extracts, essential oil and theophylline (1 mM) showed significant relaxant effects compared to those of controls. Although relaxant effect of essential oil was lower than theophylline, there was no significant difference between the effect of aqueous and ethanol extracts and that of theophylline. There was also no significant difference between the relaxant effects obtained in group 1 and 2 experiments. The results also showed parallel rightward shifts of methacholine-response curves and significant increase in EC(50) with the presence of atropine or essential oil. These results indicated bronchodilatory effects of essential oil, aqueous, and ethanol extracts from P. anisum. The results also showed that the relaxant effect of this plant is not due to an inhibitory effect of histamine (H(1)) or stimulatory effect of beta(2)-adrenergic receptors, but due to inhibitory effects on muscarinic receptors.

  2. Ochratoxin A production in aniseed-based media by selected fungal strains and in anise fruits (Pimpinella anisum L.).

    Science.gov (United States)

    Espejo, Francisco; Vázquez, Covadonga; Patiño, Belén; Armada, Sandra

    2010-05-01

    The growth conditions and ochratoxin A (OTA) production of Aspergillus strains were studied in aniseed (Pimpinella anisum L.)-based media. The results showed that methanol/NaHCO3 (50:50, v/v) mixture for extraction and competitive direct ELISA analytical method are capable of detecting low OTA concentrations in this raw material, which were confirmed by HPLC with fluorescence detection (R (2) = 0.994). In aniseed meal extract agar artificially contaminated with selected fungi, the higher OTA values obtained were 283.8 ± 28.1 μg L(-1) for A. carbonarius and between 1.7 ± 0.1 μg L(-1) and 16.5 ± 12.8 μg L(-1) for A. steynii strains. While the optimal conditions of growth for A. carbonarius and A. steynii are 28°C and 0.98 a w, the optimal production of OTA was observed at 0.99 a w for both A. carbonarius and A. steynii but at 22°C and 28°C, respectively. Except in one sample, all the aniseed samples analysed were negative for OTA natural contamination. This study demonstrates that aniseed can be a matrix capable to contamination with OTA, at least produced by A. carbonarius and A. steynii strains, regardless of the antimicrobial properties of aniseed essential oil.

  3. The influence of essential oil of aniseed (Pimpinella anisum, L.) on drug effects on the central nervous system.

    Science.gov (United States)

    Samojlik, Isidora; Mijatović, Vesna; Petković, Stojan; Skrbić, Biljana; Božin, Biljana

    2012-12-01

    Anise (Pimpinella anisum L.; Apiaceae) and its essential oil have been widely used in folk medicine, pharmacy and food industry. Since there are some data about the impact of anise on functions of central nervous system (CNS), the issue of possible interactions with drugs acting in CNS should be considered. This survey aimed to examine the influence of aniseed essential oil (EO) intake on the effects of drugs that act in CNS. The chemical profile of essential oil determined by GC-MS revealed as the main components: trans-anethole (88.49%), γ-himachalene (3.13%), cis-isoeugenol (1.99%), and linalool (1.79%). The effects of codeine, diazepam, midazolam, pentobarbital, imipramine and fluoxetine were tested in mice after 5days of peroral pretreatment with human equivalent dose of aniseed EO (0.3mg/kg). The intake of EO led to significant increase of analgesic effect of codeine. The motor impairment caused by midazolam was enhanced in the group treated by EO. The application of diazepam decreased the number and percentage of entries in open arm in elevated maze plus test in the group pretreated with EO indicating augmented effect of drug on motor activity. EO pretreatment caused significant shortage of pentobarbital induced sleeping time when compared to control. The decrease in antidepressant effect of imipramine and fluoxetine was diminished by the pretreatment with aniseed EO. Based on the results of this study we conclude that concomitant intake of aniseed EO preparations and drugs that act on CNS should be avoided due to potential herb-drug interactions, which also need further clinical confirmation.

  4. Evaluation of antidepressant-like effects of aqueous and ethanolic extracts of Pimpinella anisum fruit in mice

    Directory of Open Access Journals (Sweden)

    Zahra Shahamat

    2016-04-01

    Conclusion: The results of this study suggest that P. anisum possesses an antidepressant-like activity similar to that of fluoxetine, which has a potential clinical value for application in the management of depression.

  5. Green synthesis of silver nanoparticles using Pimpinella anisum seeds: antimicrobial activity and cytotoxicity on human neonatal skin stromal cells and colon cancer cells

    Directory of Open Access Journals (Sweden)

    AlSalhi MS

    2016-09-01

    Full Text Available Mohamad S AlSalhi,1,2 Sandhanasamy Devanesan,1,2 Akram A Alfuraydi,3 Radhakrishnan Vishnubalaji,4 Murugan A Munusamy,3 Kadarkarai Murugan,5 Marcello Nicoletti,6 Giovanni Benelli7 1Research Chair in Laser Diagnosis of Cancers, 2Department of Physics and Astronomy, 3Department of Botany and Microbiology, College of Science, 4Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Riyadh, Kingdom of Saudi Arabia; 5Division of Entomology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore, India; 6Department of Environmental Biology, Sapienza University of Rome, Rome, 7Department of Agriculture, Food and Environment, University of Pisa, Pisa, Italy Background: The present study focused on a simple and eco-friendly method for the synthesis of silver nanoparticles (AgNPs with multipurpose anticancer and antimicrobial activities. Materials and methods: We studied a green synthesis route to produce AgNPs by using an aqueous extract of Pimpinella anisum seeds (3 mM. Their antimicrobial activity and cytotoxicity on human neonatal skin stromal cells (hSSCs and colon cancer cells (HT115 were assessed. Results: A biophysical characterization of the synthesized AgNPs was realized: the morphology of AgNPs was determined by transmission electron microscopy, energy dispersive spectroscopy, X-ray powder diffraction, and ultraviolet-vis absorption spectroscopy. Transmission electron microscopy showed spherical shapes of AgNPs of P. anisum seed extracts with a 3.2 nm minimum diameter and average diameter ranging from 3.2 to 16 nm. X-ray powder diffraction highlighted the crystalline nature of the nanoparticles, ultraviolet-vis absorption spectroscopy was used to monitor their synthesis, and Fourier transform infrared spectroscopy showed the main reducing groups from the seed extract. Energy dispersive spectroscopy was used to confirm the presence of elemental silver. We evaluated the antimicrobial potential

  6. Bioactivity of Dianthus caryophyllus, Lepidium sativum, Pimpinella anisum, and Illicium verum essential oils and their major components against the West Nile vector Culex pipiens.

    Science.gov (United States)

    Kimbaris, Athanasios C; Koliopoulos, George; Michaelakis, Antonios; Konstantopoulou, Maria A

    2012-12-01

    Mosquitoes constitute a severe health problem in many areas all over the world. There are many regions of the tropics and subtropics where mosquitoes are one of the main reasons for inhibiting the economic upgrade. Except nuisance, their medical importance is another matter of attention since mosquitoes are vectors for a wide variety of vector-borne diseases. Due to disadvantages of currently used chemical control methods, it is unavoidable to search for eco-friendly new molecules. We report herein the evaluation of the larvicidal effect exhibited by essential oils of Dianthus caryophyllus, Lepidium sativum, Pimpinella anisum, and Illicium verum against late third to early fourth instar mosquito larvae of Culex pipiens. Furthermore, phytochemical analysis of plant samples revealed their major compounds to be β-caryophyllene, eugenol, eucalyptol, α-terpinyl acetate, and (E)-anethole which were also tested for their potential larvicidal activity. For D. caryophyllus and L. sativum, this was the first report on the chemical composition of their essential oils. The essential oils of I. verum and P. anisum demonstrated high larvicidal activity with a LC(50) <18 mg L(-1). The other two essential oils of D. caryophyllus and L. sativum revealed moderate larvicidal activity, displaying a LC(50) value above 50 mg L(-1). Among the pure components, the most toxic were eugenol, (E)-anethole, and α-terpinyl acetate, with LC(50) values 18.28, 16.56, and 23.03 mg L(-1), respectively. Eucalyptol (1,8 cineole) and β-caryophyllene were inactive at concentrations even as high as 100 mg L(-1), showing the least significant activity against mosquito larvae. Results allow some rationalization on the relative importance of the major compounds regarding the larvicidal activity of selected essential oils and their potential use as vector control agents.

  7. Phytochemical Profile and Evaluation of the Biological Activities of Essential Oils Derived from the Greek Aromatic Plant Species Ocimum basilicum, Mentha spicata, Pimpinella anisum and Fortunella margarita

    Directory of Open Access Journals (Sweden)

    Eleni Fitsiou

    2016-08-01

    Full Text Available Natural products, known for their medicinal properties since antiquity, are continuously being studied for their biological properties. In the present study, we analyzed the composition of the volatile preparations of essential oils of the Greek plants Ocimum basilicum (sweet basil, Mentha spicata (spearmint, Pimpinella anisum (anise and Fortunella margarita (kumquat. GC/MS analyses revealed that the major components in the essential oil fractions, were carvone (85.4% in spearmint, methyl chavicol (74.9% in sweet basil, trans-anethole (88.1% in anise, and limonene (93.8% in kumquat. We further explored their biological potential by studying their antimicrobial, antioxidant and antiproliferative activities. Only the essential oils from spearmint and sweet basil demonstrated cytotoxicity against common foodborne bacteria, while all preparations were active against the fungi Saccharomyces cerevisiae and Aspergillus niger. Antioxidant evaluation by DPPH and ABTS radical scavenging activity assays revealed a variable degree of antioxidant potency. Finally, their antiproliferative potential was tested against a panel of human cancer cell lines and evaluated by using the sulforhodamine B (SRB assay. All essential oil preparations exhibited a variable degree of antiproliferative activity, depending on the cancer model used, with the most potent one being sweet basil against an in vitro model of human colon carcinoma.

  8. Phytochemical Profile and Evaluation of the Biological Activities of Essential Oils Derived from the Greek Aromatic Plant Species Ocimum basilicum, Mentha spicata, Pimpinella anisum and Fortunella margarita.

    Science.gov (United States)

    Fitsiou, Eleni; Mitropoulou, Gregoria; Spyridopoulou, Katerina; Tiptiri-Kourpeti, Angeliki; Vamvakias, Manolis; Bardouki, Haido; Panayiotidis, Mihalis Ι; Galanis, Alex; Kourkoutas, Yiannis; Chlichlia, Katerina; Pappa, Aglaia

    2016-08-16

    Natural products, known for their medicinal properties since antiquity, are continuously being studied for their biological properties. In the present study, we analyzed the composition of the volatile preparations of essential oils of the Greek plants Ocimum basilicum (sweet basil), Mentha spicata (spearmint), Pimpinella anisum (anise) and Fortunella margarita (kumquat). GC/MS analyses revealed that the major components in the essential oil fractions, were carvone (85.4%) in spearmint, methyl chavicol (74.9%) in sweet basil, trans-anethole (88.1%) in anise, and limonene (93.8%) in kumquat. We further explored their biological potential by studying their antimicrobial, antioxidant and antiproliferative activities. Only the essential oils from spearmint and sweet basil demonstrated cytotoxicity against common foodborne bacteria, while all preparations were active against the fungi Saccharomyces cerevisiae and Aspergillus niger. Antioxidant evaluation by DPPH and ABTS radical scavenging activity assays revealed a variable degree of antioxidant potency. Finally, their antiproliferative potential was tested against a panel of human cancer cell lines and evaluated by using the sulforhodamine B (SRB) assay. All essential oil preparations exhibited a variable degree of antiproliferative activity, depending on the cancer model used, with the most potent one being sweet basil against an in vitro model of human colon carcinoma.

  9. Pharmacokinetic Herb-Drug Interaction between Essential Oil of Aniseed (Pimpinella anisum L., Apiaceae) and Acetaminophen and Caffeine: A Potential Risk for Clinical Practice.

    Science.gov (United States)

    Samojlik, Isidora; Petković, Stojan; Stilinović, Nebojša; Vukmirović, Saša; Mijatović, Vesna; Božin, Biljana

    2016-02-01

    Aniseed (Pimpinella anisum L., Apiaceae) and its essential oil (EO) have been widely used. Because there are some data about the impact of aniseed EO on drug effects, this survey aimed to assess the potential of pharmacokinetic herb-drug interaction between aniseed EO and acetaminophen and caffeine in mice. The chemical analysis (gas chromatography-mass spectrometry) of aniseed EO has confirmed trans-anethole (87.96%) as the main component. The pharmacokinetic studies of intraperitoneally (i.p.) and orally applied acetaminophen (200 mg/kg) and caffeine (20 mg/kg) were performed in mice after 5 days of oral treatment with human equivalent dose of aniseed EO (0.3 mg/kg/day). The analysis of pharmacokinetic data showed that in the group treated by aniseed EO, the significant decrease in the peak plasma concentration of acetaminophen after oral application (p = 0.024) was revealed when compared with control group and the reduction of systemic exposure to the drug after oral application (74 ± 32% vs. 85 ± 35% in the control) was noted. The bioavailability of orally applied caffeine was also significantly decreased (p = 0.022) after the EO treatment in comparison with the control (57 ± 24% vs. 101 ± 29%). Therefore, the compromised therapeutic efficacy of acetaminophen and caffeine during the usage of aniseed EO preparations should be considered.

  10. Chemical Composition and Zootechnical Effects of Essebtial Oil of Fennel (Foeniculum Vulgare Mill and Anise (Pimpinella Anisum L. on Turkey

    Directory of Open Access Journals (Sweden)

    Ould sidi moctar Yacoub

    2015-04-01

    Full Text Available The main aim of this study was to investigate the effects of the dietary oils from fennel and anise on the zootechnical performances of Turkey. The essential oils were analyzed by gas chromatography coupled to mass spectrometry. One Hundred turkey males meat strain BUT 10 (Meleagris gallopavo, of one day old, were weighed and randomly allotted into 5 groups of 20 turkeys each. These groups were fed, for 35 days, the basal diet (Control or the basal diet supplemented with 0.2 g/kg of fennel oil (EOFVD1, 0.5 g/kg of fennel oil (EOFVD2, 0.2 g/kg of anise oil (EOPAD1 and 0.5 g/kg of anise oil (EOPAD2. The mean body weight of EOFVD2 group (807.85 g was higher than other groups at the end of the treatment (P<0.05. Likewise, mean body weight of the EOPAD1 group (782.45 g was different from those of EOFVD1 (769.89 g, EOPAD2 (756.77 g and Control group (768.35 g (P<0.01. The results related to the average day weight gain (ADG indicated that subjects belonging to the batches treated with EOFVD2, EOPAD1 and EOFVD1, expressed the best performance with ADG respective of 79.46±4.03 g/d, 51.12±27.88 g/d, 50.47±26.94 g/d, followed by control group (49.76±26.67 g/d, against 49.98 ±28.46 g/d for the EOPAD2 group with a difference which is statistically significant (P<0.05. These results concluded that supplementing diets with essential oils of fennel and anise, which improved body weight, could be interesting feed additives for growth promotion turkey.

  11. Effect of Water Based Infusion of Aloe barbedensis, Pimpinella anisum, Berberis lycium, Trigonella foenum-graecum and Allium sativum on The Performance of Broiler Chicks

    Directory of Open Access Journals (Sweden)

    Fazal Raziq, Sarzamin Khan*, Naila Chand, Asad Sultan, Muhammad Mushtaq, Rafiullah, Sayed Muhammad Suhail1 and Alam Zeb2

    2012-10-01

    Full Text Available Present study explored the potentials of medicinal plants (Aloe barbedensis, Pimpinella anisum, Berberis lycium, Trigonella foenum-graecum, Allium sativum mixture in broiler chicks at a ratio of 1:3:1:2:1, respectively. For this purpose 240 chicks were randomly assigned into four major groups, namely; A, B, C and D. Each group was further divided into two subgroups with three replicates of 10 chicks each. One of the subgroups was vaccinated against Newcastle disease (ND, Infectious bronchitis (IB and Infectious bursal disease (IBD according to locally adopted vaccination schedule keeping the other subgroup as non vaccinated control. Experimental birds in groups A, B, and C were provided with mentioned infusion @ 20, 10 and 5ml per liter of drinking water while group D was maintained as control. Relevant data were recorded throughout the experiment and or at the termination of the experiment and subjected to statistical analysis. Significantly low mean feed intake (3258.3g and better FCR (1.87 was recorded in group A. Other parameters like weight gain (1739.7g, antibody titer against ND (7.1, IBD (3300.5 and high density lipoprotein (71.6 g/dl were significantly increased in chicks from group A. Similarly, blood cholesterol (145.6 g/dl, triglyceride (145.8 g/dl and low density lipoprotein (57.5 g/dl were significantly reduced in group A. Overall vaccinated group had higher (P<0.05 antibody titer 7.2, 1796.2, 3202.8 against ND, IB and IBD, respectively compared to non-vaccinated group and had no influence on lipid profile. It was concluded that the infusion from the above plants in mentioned composition may not only be effectively used for improved broiler performance and better immunity but also to reduce the cholesterol level.

  12. Variabilité de la composition chimique des huiles essentielles de Coriandrum sativum L et Pimpinella anisum L au cours de développement végétatif.

    Directory of Open Access Journals (Sweden)

    K. FYAD

    2013-03-01

    Full Text Available Dans cette étude, Nous nous sommes intéressés à quantifier et identifier les huiles essentielles extraites par hydrodistillation des parties aériennes fraîches des deux plantes aromatiques : Coriandrum sativum L et Pimpinella anisum L, aux cours de leur cycle de croissance (avant, pendant et après floraison ; cultivées dans un champ à l’université de Béchar. La récolte a été faite au cours de l'année 2011. Les huiles essentielles obtenues à partir des parties aériennes fraîches durant le cycle végétatif, ont été analysés par CPG.

  13. Evaluation of asepsis, agar concentration and culture medium composition methods for in vitro propagation of Pimpinella anisum (Linn. – Apiaceae Avaliação de métodos de esterilização, concentração de ágar e composição de meio de cultura para propagação in vitro de Pimpinella anisum (Linn. – Apiaceae

    Directory of Open Access Journals (Sweden)

    Gabriela Tambosi

    2010-12-01

    Full Text Available Pimpinella anisum, is a plant species popularly used as treatment for different diseases, although no studies exist which could prove its pharmacological activity. This species has no known methods of asepsis and adequate conditions to conduct in vitro propagation trials. To assess the most efficient method of seed sterilization and the most favorable concentration of agar and MS salts, five tests of asepsis and tests to evaluate the dry mass increase of germinated seed in 3g/L and 6g/L of agar and MS in concentrations of 0%, 25%, 50%, 75% and 100%, were carried out. The samples that were immersed in a concentration of 2% sodium hypochlorite for 20 minutes and then washed in distilled water produced 100% of sterile plantlets. The greatest increase in dry mass was obtained with 75% and 100% of MS salts. Furthermore, the different concentrations of agar did not influence the percentage of the plantlets dry mass. Pimpinella anisum é uma planta popularmente utilizada como medicinal para diversas enfermidades, embora não existam estudos que comprovem sua atividade farmacológica. A espécie não apresenta metodologias conhecidas de assepsia e condições adequadas para experimentos de propagação in vitro. Para avaliar a forma mais eficiente de esterilização de sementes da espécie e melhores concentrações de ágar e sais do meio MS, foram feitos cinco testes de assepsia, bem como testes de avaliação do aumento de massa seca a partir de sementes germinadas em ágar 3g/L e 6g/L e MS nas concentrações 0%, 25%, 50%, 75% e 100%. A imersão em solução hipoclorito de sódio 2% por 20 minutos e lavagem em água destilada promoveu 100% de plântulas estéreis. O maior aumento de massa seca foi obtido com concentração de sais do meio MS de 75% e 100%, e as diferentes concentrações de ágar não influenciaram a obtenção de maior percentual de massa de plântulas cultivadas.

  14. Effect of adding crushed Pimpinella anisum, Nigella sativa seeds and Thymus vulgaris mixture to antibiotics-free rations of vaccinated and non-vaccinated male broilers on growth performance, antibody titer and haematological profile

    Directory of Open Access Journals (Sweden)

    Mamoun Z. Athamneh

    2010-04-01

    Full Text Available This research explores an experimental study conducted to investigate the effect of crushed Pimpinella anisum (PA, Nigella sativa (NS seeds and Thymus vulgaris (TV mixture as a feed additive on growth performance and mortality rate (MR, selected antibodies titer (Ab’s and blood hematological profile of vaccinated and non-vaccinated Lohman male broiler chicks fed free-antibiotics ration. A total of 400 one-day old chicks were distributed into 16 groups (4 treatment x 4 replicates x 25chicks. The experiment lasted from one to 42 days of age. The statistical findings of this experiment prove that the use of medicinal plants mixture improves live body weight, body weight gain, feed conversion ratio and MR of vaccinated male broilers at 21 and 42 days of age. antibodies titer against infectious bronchitis and infectious bursal disease of non-vaccinated and vaccinated male broilers were significantly improved at 21 and 42 days as a result of the addition of medicinal plant mixture to the basal ration. Concerning Newcastle disease, the use of PA, NS and TV mixture did not reflect in any additional improvement of Ab's than vaccines did. The addition of medicinal plants mixture increases WBC's, RBC's, thrombocytes count and Hb concentration of vaccinated and non-vaccinated male broilers at 21 days of age. Meanwhile, heterophils, lymphocytes and monocytes of vaccinated male broilers (VMB were significantly improved by adding medicinal plant mixture to their basal diet. Moreover, at 42 days of age the use of PA, NS seeds and TV mixture indicate significant increase in total WBC’s, lymphocytes and monocytes and monocytes count of VMB and non-vaccinated male broiler (NVMB. No significant differences were noticed in RBC’s and Hct as a result of feeding crushed medicinal plants mixture.

  15. Aqueous suspension of anise “Pimpinella anisum” protects rats against chemically induced gastric ulcers

    OpenAIRE

    2007-01-01

    AIM: To substantiate the claims of Unani and Arabian traditional medicine practitioners on the gastroprotective potential effect of a popular spice anise, “Pimpinella anisum L.” on experimentally-induced gastric ulceration and secretion in rats.

  16. Gas chromatographic-mass spectrometric analysis of essential oils from Pimpinella species gathered from Central and Northern Turkey.

    Science.gov (United States)

    Tabanca, Nurhayat; Demirci, Betul; Ozek, Temel; Kirimer, Nese; Baser, K Husnu Can; Bedir, Erdal; Khan, Ikhlas A; Wedge, David E

    2006-06-09

    Essential oils from 15 Pimpinella species were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) techniques. One species, Pimpinella anisum, in which only fruits were evaluated, was also included in the study. A total of 140 different compounds were identified and significant qualitative and quantitative differences were observed among the samples. Pimpinella essential oils were characterized as having mono-, sesqui- and trinorsesquiterpenoids, propenylphenols, and pseudoisoeugenols. Trinorsesquiterpenoids and phenylpropanoids appear to be chemical markers of Pimpinella species analyzed thus far. Essential oils obtained from Pimpinella roots share the same principal compound, epoxypseudoisoeugenyl-2-methylbutyrate at concentrations from 20 to 82.6%.

  17. Terpene hydrocarbons in Pimpinella anisum L.

    Science.gov (United States)

    Burkhardt, G; Reichling, J; Martin, R; Becker, H

    1986-06-20

    The essential oil of anise (fruits and shoots) was investigated focusing on the composition of the hydrocarbon fraction. Several sesquiterpenes were identified by GC-MS and the relative composition of the fractions was established by GC analysis. gamma-Himachalene and the diterpene neophytadiene were isolated by TLC and column chromatography at low temperatures. Their structures were determined by MS and NMR including 1H-1H correlated COSY and NOE experiments.

  18. [Isolation and identification of flavon(ol)-O-glycosides in caraway (Carum carvi L.), fennel (Foeniculum vulgare Mill.), anise (Pimpinella anisum L.), and coriander (Coriandrum sativum L.), and of flavon-C-glycosides in anise. I. Phenolics of spices (author's transl)].

    Science.gov (United States)

    Kunzemann, J; Herrmann, K

    1977-07-29

    The flavonoid constituents of various spices were separated by means of chromatography on cellulose colums, and the following compounds were obtained crystalline: Quercetin 3-glucuronide from caraway, fennel, anise, and coriander; isoquercitrin from caraway and fennel; rutin from fennel and anise; quercetin 3-O-caffeylglucoside and kaempferol 3-glucoside from caraway; quercetin 3-arabinoside from fennel, and luteolin 7-glucoside, isoorientin and isovitexin from anise. Other constitutents which were however not obtained crystalline, but which could be identified by the usual procedures were kaempferol 3-glucuronide and kaempferol 3-arabinoside in fennel, apigenin 7-glucoside and a luteolin glycoside in anise, and isoquercitrin and rutin in coriander. The glycosides contained in the fruit of the four spices also occur in the leaves. Leaves of caraway and fennel in addition contain isorhammetin glycosides in low concentration.

  19. Endogenous cytokinins during embryogenesis in an anise cell culture (Pimpinella anisum L.).

    Science.gov (United States)

    Ernst, D; Oesterhelt, D; Schäfer, W

    1984-05-01

    Endogenous levels of cytokinins in an anise cell culture were determined by the use of radioimmunoassay and gas chromatography-mass spectrometry in combination with single-ion monitoring, during proembryonal and embryonal development. In both cultures the highest cytokinin levels were correlated with logarithmic growth (embryonal: isopentenyladenosine, 4 ng g(-1) fresh weight [FW]; isopentenyladenine, 1.4 ng g(-1) FW; zeatin, 3.6 ng g(-1) FW; proembryonal: isopentenyladenosine, 58.3 ng g(-1) FW; isopentenyladenine, 7.9 ng g(-1) FW; zeatin 11.1 ng g(-1) FW). The proembryonic culture medium but not the embryonic culture medium contained isopentenyladenosine up to 28 pg ml(-1) during logarithmic growth. No correlation between different embryonic stages and the endogenous cytokinin level was obvious.

  20. Effects of fertilizer and plant density on yield and quality of anise (Pimpinella anisum L.

    Directory of Open Access Journals (Sweden)

    Faravani Mahdi

    2013-01-01

    Full Text Available In order to understand the effect of organic fertilizer on yield of anise, an experiment was conducted in the form of split-plot in randomized complete block design with three replications in Mashhad, Khorasan Agriculture and Natural Resource Research Center. Four treatments of fertilization: the control, vermicompost - 5 t/ha, cow manure - 25 t/ha, and mineral fertilizer (NPK - 60 kg/ha (the same rate of each nutrient were applied as the main factor. The second factor was plant density, applied at three levels: 17, 25, and 50 plants/m2. The results showed a significant effect of fertilizer on the number of umbels per plant, number of umbellets per umbel and canopy cover. Plant density had a significant effect on grain yield, biological yield, the number of lateral branches, essential oil percentage and yield of essential oil. Seed and essential oil yield were the highest in the case of the application of vermicompost and plant densities of 50 and 25 plants/m2 respectively.

  1. Effect of exogenous cytokinins on growth and somatic embryogenesis in anise cells (Pimpinella anisum L.).

    Science.gov (United States)

    Ernst, D; Oesterhelt, D

    1984-05-01

    A cell culture of anise was grown in the presence or absence of 2,4-dichlorophenoxyacetic acid (2,4-D). Application of isopentenyladenine or isopentenyladenosine (4·10(-8) to 4·10(-7) M) to the proembryonic culture (+2,4-D) yielded an increase of the cell density, in contrast to a proembryonic culture grown without exogenous application of cytokinins. Embryogenesis was induced by transferring the cells to a hormone-free medium. Embryo development was promoted by isopentenyladenine and isopentenyladenosine (5·10(-8) to 5·10(-7) M), higher concentrations (5·10(-6) M) inhibited embryogenesis. The effect of cytokinins on embryogenesis was only promotive until the third day of culture, i.e. coincident with cell growth rather than differentiation.

  2. Changes of cytokinin nucleotides in an anise cell culture (Pimpinella anisum L.) during growth and embryogenesis.

    Science.gov (United States)

    Ernst, D; Oesterhelt, D

    1985-05-01

    Endogenous levels of cytokinin nucleotides in an anise cell culture were determined during proembryonal, as well as embryonal development. In both cultures the maximum level of isopentenyladenine nucleotides was found during the first four days of incubation which correlated with the beginning of logarithmic growth (embryonal: 8 ng g(-1) tresh weight; proembryonal: 17.4 ng g(-1) fresh weight). The concentration of zeatin nucleotides remained constant at a very low level. The present data and those of Ernst et al. (1984) and Ernst and Oesterhelt (1984) are concerned in ascribing a major role to cytokinins in cell division, but not in embryo differentiation.

  3. Etude phytochimique et activité antioxydante de l’extrait aqueux de Pimpinella anisum L.

    Directory of Open Access Journals (Sweden)

    Amina Bekara

    2016-08-01

    Full Text Available L’étude expérimentale a été réalisée sur l’extrait aqueux lyophilisé des graines d’anis vert. La composition chimique de l’extrait a été analysé par la chromatographie liquide à haute performance et spectroscopie infra Rouge à transformée de Fourier. Les résultats de cette étude montre que l’extrait aqueux renferme une certaine quantité de polyphénols (2,38±0,01 mg équivalent d’acide gallique /g de poids sec de la plante, flavonoïdes (8,06 ± 0,08 mg équivalent de Quercétine / g de poids sec de la plante et tannins (0,40±0,004 mg équivalent d’acide gallique / g de poids sec de la plante avec l’absence d’autres groupes de composés bioactifs tel que les saponins et les stéroïdes. L’anéthole reste le composé majoritaire dans l’extrait aqueux obtenu à partir des graines d’anis vert. L’extrait de cette plante possède un pouvoir antioxydant moyen en comparaison avec les antioxydants de référence.

  4. Antimicrobial constituents of Foeniculum vulgare.

    Science.gov (United States)

    Kwon, Yong Soo; Choi, Won Gyu; Kim, Won Jun; Kim, Woo Kyung; Kim, Myong Jo; Kang, Won Hee; Kim, Chang Min

    2002-04-01

    A phenyl propanoid derivative, dillapional(1) was found to be a antimicrobial principle of the stems of Foeniculum vulgare (Umbelliferae) with MIC values of 125, 250 and 125/ against Bacillus subtilis, Aspergillus niger and Cladosporium cladosporioides, respectively. A coumarin derivative, scopoletin(2) was also isolated as marginally antimicrobial agent along with inactive compounds, dillapiol(3), bergapten(4), imperatorin(5) and psolaren(6) from this plant. The isolates 1-6 were not active against the Escherichia coli.

  5. Pimpinella anisum in modifying the quality of life in patients with functional dyspepsia: A double-blind randomized clinical trial

    Directory of Open Access Journals (Sweden)

    S Ashraffodin Ghoshegir

    2014-01-01

    Full Text Available Background: We aimed to assess the effects of anise on quality of life (QOL of patients with functional dyspepsia (FD in a double-blind randomized clinical trial. Materials and Methods: Of 180 patients attending the gastroenterology clinic, 107 ones with the diagnosis of postprandial distress syndrome according to Rome III criteria were enrolled. They were randomized into two groups, anise and placebo. Anise group involved 47 patients and received anise powders, 3 g after each meal (3 times/day for 4 weeks. Control group had 60 patients who received placebo powders (cornstarch, 3 g after each meal (3 times/day for 4 weeks. The QOL was assessed by short-form (SF-36 questionnaire. Mean scores of eight health domains of the two groups were compared at baseline and at the end of study. Results: The age, sex, body mass index, smoking history, tea and coffee drinking patterns of the two groups were not significantly different. All domains of SF-36 were similar between the two groups at baseline but were significantly different at week 12. At baseline, mean score of physical component summary was 159 in placebo group and 167 in anise group (P = 0.1. At week 12, the score was 141 in placebo group and 251 in anise group (P = 0.0001. Mean baseline score of mental component summary was 172 and 165 in placebo and anise groups, respectively (P = 0.1. At week 12, the score was 135 in placebo group and 233 in anise group (P = 0.0001. Conclusion: The current study revealed the effectiveness of anise in improvement of QOL in patients with FD.

  6. Application of 2D-HPLC/taste dilution analysis on taste compounds in aniseed (Pimpinella anisum L.).

    Science.gov (United States)

    Pickrahn, Stephen; Sebald, Karin; Hofmann, Thomas

    2014-09-24

    This is the first application of fully automated, preparative, two-dimensional HPLC combined with sensory analysis for taste compound discovery using a sweet and licorice-like bitter-tasting aniseed extract as an example. Compared to the traditional iterative fractionation of food extracts by sensory-guided sequential application of separation techniques, the fully automated 2D-HPLC allowed the comprehensive separation of the aniseed extract into 256 subfractions and reduced the fractionation time from about 1 week to <1day. Using a smart sensory strategy to locate high-impact fractions, e.g., by evaluating first-dimension fractions by reconstituting them from second-dimension subfractions, followed by straightforward application of the taste dilution analysis on the individual second-dimension subfractions revealed the sweet-tasting trans-anethole and the bitter-tasting trans-pseudoisoeugenol 2-methylbutyrate, showing recognition thresholds of 70 and 68 μmol/L, respectively, as the primary orosensory active compounds in aniseed. 2D-HPLC combined with smart sensory analysis seems to be a promising strategy to speed the discovery of the key players imparting the attractive taste of foods.

  7. p-Anisaldehyde: acaricidal component of Pimpinella anisum seed oil against the house dust mites Dermatophagoides farinae and Dermatophagoides pteronyssinus.

    Science.gov (United States)

    Lee, Hoi-Seon

    2004-03-01

    The acaricidal activity of anise seed oil-derived p-anisaldehyde and commercially available components of anise seed oil was examined against Dermatophagoides farinae and D. pteronyssinus and compared with those of the synthetic acaricides, benzyl benzoate and N,N-diethyl- m-toluamide (DEET). On the basis of LD 50 values, the compound most toxic to D. farinae adults was p-anisaldehyde (1.11 microg/cm2) followed by benzyl benzoate (9.32 microg/cm2), DEET (36.84 microg/cm2), 3-carene (42.10 microg/cm2), and estragol (43.23 microg/cm2). Against D. pteronyssinus adults, p-anisaldehyde (0.98 microg/cm2) was much more effective than benzyl benzoate (6.54 microg/cm2), DEET (17.79 microg/cm2), 3-carene (39.84 microg/cm 2), and estragol (40.11 microg/cm2). p-Anisaldehyde was about 8.4 and 6.7 times more toxic than benzyl benzoate against D. farinae and D. pteronyssinus adults, respectively. The results suggested that p-anisaldehyde may be useful as a lead compound for the development of new agents for the selective control of house dust mites.

  8. Phenolic content in differentiated tissue cultures of untransformed and Agrobacterium-transformed roots of anise (Pimpinella anisum L.).

    Science.gov (United States)

    Andarwulan, N; Shetty, K

    1999-04-01

    To investigate the role of differentiation of anise tissue cultures on total phenolic and anethole contents, benzylaminopurine- and thidiazuron-induced shoot cultures were generated from roots of the A-8 clonal line and its Agrobacterium rhizogenes-induced genetically transformed derivative JB-10. Embryogenic cultures were induced following 2,4-D treatment. Root cultures were multiplied on hormone-free medium. The effect of proline on differentiation and phenolic synthesis was also investigated. GC/MS studies indicate that anethole was not produced in root or other differentiated cultures. The predominant phenolic metabolite, however, was an anethole precursor, epoxypseudoisoeugenol-2-methylbutyrate (EPB). Total phenolics and EPB contents were highest in root cultures, which also correlated with higher proline content. Embryo and shoot cultures had reduced phenolic level and EPB and proline contents. Antioxidant activity in all differentiating cultures was high on day 60 compared to that on day 30, and there was no significant difference between differentiating tissues. This indicated that antioxidant protection might be linked not only to phenolics but to other nonphenolic metabolites as well.

  9. Supercritical extraction of essential oil from aniseed (Pimpinella anisum L) using CO2: solubility, kinetics, and composition data.

    Science.gov (United States)

    Rodrigues, Vera M; Rosa, Paulo T V; Marques, Marcia O M; Petenate, Ademir J; Meireles, M Angela A

    2003-03-12

    Supercritical fluid extraction (SFE) from aniseed using carbon dioxide was performed at 30 degrees C and pressures of 80-180 bar. The chemical composition of the SFE extract was determined by GC-MS; the quantitative analysis was done by GC-FID and TLC. The total amount of extractable substances or global yield (mass of extract/mass of feed) for the SFE process varied from 3.13 to 10.67% (mass). The solubilities of the anise essential oil in CO(2) were 0.0110, 0.0277, 0.0143, and 0.0182 kg of solute/kg of CO(2) at 80, 100, 140, and 180 bar, respectively. The major compounds identified and quantified in the extracts were anethole ( approximately 90%), gamma-himachalene (2-4%), p-anisaldehyde (<1%), methylchavicol (0.9-1.5%), cis-pseudoisoeugenyl 2-methylbutyrate ( approximately 3%), and trans-pseudoisoeugenyl 2-methylbutyrate ( approximately 1.3%). The Sovová model described quite well the experimental overall extraction curves.

  10. Pimpinella anisum in modifying the quality of life in patients with functional dyspepsia: A double-blind randomized clinical trial

    Science.gov (United States)

    Ghoshegir, S. Ashraffodin; Mazaheri, Mohammad; Ghannadi, Alireza; Feizi, Awat; Babaeian, Mahmoud; Tanhaee, Maryam; Karimi, Mehrdad; Adibi, Peyman

    2014-01-01

    Background: We aimed to assess the effects of anise on quality of life (QOL) of patients with functional dyspepsia (FD) in a double-blind randomized clinical trial. Materials and Methods: Of 180 patients attending the gastroenterology clinic, 107 ones with the diagnosis of postprandial distress syndrome according to Rome III criteria were enrolled. They were randomized into two groups, anise and placebo. Anise group involved 47 patients and received anise powders, 3 g after each meal (3 times/day) for 4 weeks. Control group had 60 patients who received placebo powders (cornstarch), 3 g after each meal (3 times/day) for 4 weeks. The QOL was assessed by short-form (SF)-36 questionnaire. Mean scores of eight health domains of the two groups were compared at baseline and at the end of study. Results: The age, sex, body mass index, smoking history, tea and coffee drinking patterns of the two groups were not significantly different. All domains of SF-36 were similar between the two groups at baseline but were significantly different at week 12. At baseline, mean score of physical component summary was 159 in placebo group and 167 in anise group (P = 0.1). At week 12, the score was 141 in placebo group and 251 in anise group (P = 0.0001). Mean baseline score of mental component summary was 172 and 165 in placebo and anise groups, respectively (P = 0.1). At week 12, the score was 135 in placebo group and 233 in anise group (P = 0.0001). Conclusion: The current study revealed the effectiveness of anise in improvement of QOL in patients with FD. PMID:25709650

  11. Hepatoprotective effect of Foeniculum vulgare essential oil.

    Science.gov (United States)

    Ozbek, H; Uğraş, S; Dülger, H; Bayram, I; Tuncer, I; Oztürk, G; Oztürk, A

    2003-04-01

    Hepatoprotective activity of Foeniculum vulgare (fennel) essential oil (FEO) was studied using carbon tetrachloride (CCl(4)) induced liver injury model in rats. The hepatotoxicity produced by acute CCl(4) administration was found to be inhibited by FEO with evidence of decreased levels of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and bilirubin. The results of this study indicate that FEO has a potent hepatoprotective action against CCl(4)-induced hepatic damage in rats.

  12. REGENERATION OF Pimpinella pruatjan THROUGH SOMATIC EMBRYOGENESIS

    Directory of Open Access Journals (Sweden)

    I. Roostika

    2016-10-01

    Full Text Available Pruatjan (Pimpinella pruatjan Molk. is an Indonesian endangered plant which has various medicinal properties such as aphrodisiac, diuretic, and tonic. The plant is commonly harvested from its natural habitat, therefore it becomes endangered. Regeneration of pruatjan through organogenesis has been studied, but its shoot multiplication was very low (5 shoots per explant. The study aimed to investigate the best regeneration technique of pruatjan through somatic embryogenesis. This research was conducted at the tissue culture laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development in 2004-2005. Callus formation of pruatjan was induced from the petioles and leaves in Driver and Kuniyaki’s (DKW based medium containing 2,4-D combined with picloram at the level of 0.5, 1.0, 1.5, and 1.5 ppm. Embryogenic calli were then transferred into embryo development medium in two ways. First, they were directly transferred into media containing IBA/NAA at the level of 0.5, 1.0, and 1.5 ppm. Second, they were indirectly transferred into media containing 2.0 ppm 2,4-D and 0.3% casein hydrolysate prior to the IBA/ NAA media. Parameters evaluated were fresh weight, dry weight, time initiation of embryogenic callus formation, and total number of embryos. The result showed that calli of pruatjan were successfully induced from the petioles and leaves. The best calli were induced from the leaves in the DKW medium containing 2.0 ppm 2,4-D and 0.5 ppm picloram. Embryo development of the calli was best if they were first grown in the media containing 2.0 ppm 2,4-D and 0.3% casein hydrolysate then transferred to the IBA/NAA media. The total number of somatic embryos was counted up to 103 on the medium containing 1.5 ppm IBA. This study indicated that pruatjan somatic embryogenesis regeneration required three different media, i.e. for callus induction, development and maturation, and for

  13. Environ: E00712 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available ruits Obtained by steam distillation Major component: Anethole [CPD:C10428] Essen...e, Limonene [CPD:C06078] Pimpinella anisum [TAX:271192] Same as: D02945 Apiaceae Pimpinella anisum crushed f

  14. Investigating Genetic Diversity of Foeniculum Vulgare Mill using Molecular Markers

    Directory of Open Access Journals (Sweden)

    Omid Jadidi

    2016-06-01

    Full Text Available Medicinal plants are considered valuable genetic resources in Iran. One of these medicinal as well as spice plants is Foeniculum Vulgare Mill from Umbellifetae family used in different industries such as food, medicine, and cosmetics. It seems that due to different climate conditions in Iran this plant represents a high and valuable genetic diversity; therefore, management of genetic resources protection and obtaining information about genetic diversity will help awareness of evolution processes as well as genetic erosion of this valuable plant. Genetic diversity in local masses of Foeniculum Vulgare Mill can be investigated using molecule markers such as AFLP, RAPD, ISSR, SRAP, RFLP, and so on. In investigation of over 30 ecotype of local Foeniculum Vulgare Mill, different markers have shown that mean polymorphic content (PIC is about 36% and mean genetic diversity is estimated about 40% in different samples. Data obtained from molecule software analyses help to categorize Foeniculum Vulgare Mill genotype in different groups based on climate and geographical conditions. Principle components analysis (PCOA has also confirmed the results of cluster analysis. Dendrogram obtained by cluster analysis based on similarity coefficient of simple matching (SM and UPGMA algorithm can also categorize population of Foeniculum Vulgare Mill in different groups. Results of molecular variance analysis (AMOVA have shown that most genetic variance between geographical groups can be seen in populations. In general, according to investigations, there is a significant genetic diversity regarding agronomic and molecular traits of Foeniculum Vulgare Mill masses in Iran and knowing this genetic diversity will help in breeding programs, complementary studies, categorization, and so on.

  15. PENGARUH INFUS BUAH Foeniculum vulgare Mill PADA SIKLUS ESTRUS DAN BOBOT UTERUS TIKUS PUTIH

    OpenAIRE

    Sa'roni Sa'roni; Imono A. Donatus

    2012-01-01

    Buah Foeniculum vulgare Mill (adas) secara tradisional untuk obat dan campuran jamu pengatur haid. Diduga buah Foeniculum vulgare Mill mempunyai pengaruh pada sistem reproduksi. Oleh karena itu, kami melakukan penelitian pengaruh infus buah Foeniculum vulgare Mill pada siklus estrus dan bobot uterus tikus putih.Infus diberikan secara oral sekali sehari selama 12 hari dengan dosis setara dengan 7,3 mg, 73 mg dan 219 mg/100 g bobot badan. Selama pemberian bahan, siklus estrus diperiksa setiap h...

  16. Fumigant activity of plant essential oils and components from horseradish (Armoracia rusticana), anise (Pimpinella anisum) and garlic (Allium sativum) oils against Lycoriella ingenua (Diptera: Sciaridae).

    Science.gov (United States)

    Park, Ii-Kwon; Choi, Kwang-Sik; Kim, Do-Hyung; Choi, In-Ho; Kim, Lee-Sun; Bak, Won-Chull; Choi, Joon-Weon; Shin, Sang-Chul

    2006-08-01

    Plant essential oils from 40 plant species were tested for their insecticidal activities against larvae of Lycoriella ingénue (Dufour) using a fumigation bioassay. Good insecticidal activity against larvae of L. ingenua was achieved with essential oils of Chenopodium ambrosioides L., Eucalyptus globulus Labill, Eucalyptus smithii RT Baker, horseradish, anise and garlic at 10 and 5 microL L(-1) air. Horseradish, anise and garlic oils showed the most potent insecticidal activities among the plant essential oils. At 1.25 microL L(-1), horseradish, anise and garlic oils caused 100, 93.3 and 13.3% mortality, but at 0.625 microL L(-1) air this decreased to 3.3, 0 and 0% respectively. Analysis by gas chromatography-mass spectrometry led to the identification of one major compound from horseradish, and three each from anise and garlic oils. These seven compounds and m-anisaldehyde and o-anisaldehyde, two positional isomers of p-anisaldehyde, were tested individually for their insecticidal activities against larvae of L. ingenua. Allyl isothiocyanate was the most toxic, followed by trans-anethole, diallyl disulfide and p-anisaldehyde with LC(50) values of 0.15, 0.20, 0.87 and 1.47 microL L(-1) respectively.

  17. Chemical Composition of the Essential Oil from Aerial Parts of Javanian Pimpinella pruatjan Molk. and Its Molecular Phylogeny

    Directory of Open Access Journals (Sweden)

    Agustina D. R. Nurcahyanti

    2016-07-01

    Full Text Available The species-rich and diverse genus Pimpinella is mainly distributed in Europe and Asia; a few species occur in Africa. Yet, the Javanian Pimpinella, P. pruatjan, which has been used as an aphrodisiac in Indonesian traditional medicine, was studied for the first time in the context of chemical composition, as well as phylogeny analysis and antimicrobial activity. We examined the chemical composition of the essential oil (EO from aerial parts of P. pruatjan by gas liquid chromatography-mass spectrometry (GLC-MS. The main component of EO was (Z-γ-bisabolene. Several oxygenated monoterpenes, oxygenated sesquiterpenes, and sesquiterpenes were also detected. The genetic relationship of Pimpinella pruatjan Molk. to other Pimpinella species was reconstructed using nucleotide sequences of the nuclear DNA marker ITS (Internal Transcribed Spacer. P. pruatjan clusters as a sister group to the African Pimpinella species. The EO did not exhibit an apparent antimicrobial activity.

  18. AGASTACHE FOENICULUM – A PERSPECTIVE SOURCE OF MEDICAL PRODUCTS

    Directory of Open Access Journals (Sweden)

    V. V. Chumakova

    2013-01-01

    Full Text Available A phytochemical study has been conducted to explore Agastache foeniculum (Lamiaceae, which is widely used in oriental medicine for colds, inflammation of the gastrointestinal tract and urinary system; exter-nally for fungal dermatitis, seborrhea, hair growth and strengthening. In the course of the experiment methods of chemical (such as alkalimetry, permanganatometriya, chelatometry and physico-chemical (such as mass spec-trometry coupled with GC, differential UV spectrophotometry, HPLC, planar chromatography analysis have been applied. The established range of biologically active compounds, especially essential oils and phenolic compounds, indicates the possibility of using Agastache foeniculum herb as a raw material source for producing drugs with antioxidant, antimicrobial, antimycotic and pilotropnym effect

  19. Isolation of endophyic bacteria from purwoceng (Pimpinella alpina Kds.

    Directory of Open Access Journals (Sweden)

    Tri Widayat

    2012-09-01

    Full Text Available AbstrakLatar belakang: Purwoceng (Pimpinella alpina Kds. merupakan tanaman obat langka yang berkhasiat sebagai afrodisiak. Tanaman yang mengandung kumarin tersebut berinteraksi dengan bakteri endofi t. Senyawa kumarin dalam industri dimanfaatkan untuk bahan aditif makanan dan parfum. Penelitian ini bertujuan untuk mendapatkan bakteri endofi t dari tanaman purwoceng, menganalisis kemampuan tumbuh bakteri endofi t dalam medium yang mengandung kumarin, dan pengaruh bakteri tersebut terhadap jumlah kumarin dalam medium pertumbuhan.Metode: Isolasi bakteri endofi t dilakukan secara langsung dari akar dan daun purwoceng. Seleksi bakteri endofi t penghasil kumarin dilakukan berdasarkan kemampuan bertahan hidup pada medium cair ammoniumsalt sugar (ASS yang mengandung infusa herba purwoceng. Pengaruh bakteri terhadap jumlah kumarin di dalam medium pertumbuhan diuji melalui percobaan kultivasi isolat bakteri terpilih pada medium yang sama. Jumlah kumarin dalam kultur dideteksi menggunakan teknik kromatografi lapis tipis (KLT.Hasil: Sembilan isolat bakteri endofi t yang berhasil diisolasi dari akar dan daun tanaman purwoceng mampu bertahan hidup pada medium basal yang diberi infusa herba purwoceng dengan waktu generasi (g 2,7-5,07jam dan kecepatan pertumbuhan spesifi k (μ 0,14-0,26/jam. Kultivasi isolat terpilih menunjukkan bahwa BAP5 menghasilkan senyawa dengan Rf 0,27 yang diduga sebagai turunan kumarin. Bakteri BAP5 mampu tumbuh dengan jumlah kumarin 1072 arbitrary unit (AU dalam medium.Kesimpulan: Bakteri endofi t dapat diisolasi dari tanaman purwoceng dan secara in vitro mampu mempertahankan jumlah kumarin yang terkandung di dalam medium. (Health Science Indones 2012;1:31-6Kata kunci: bakteri endofi t, purwoceng, Pimpinella alpina Kds, kumarinAbstractBackground: Purwoceng (Pimpinella alpina Kds. is a medicinal plant species used as aphrodisiac. Like any other plants, the coumarin containing plant probably interacts with endophytic bacteria. Coumarin

  20. Phytotoxic Activities of Mediterranean Essential Oils

    Directory of Open Access Journals (Sweden)

    Luiz Fernando Rolim de Almeida

    2010-06-01

    Full Text Available Twelve essential oils from Mediterranean aromatic plants were tested for their phytotoxic activity, at different doses, against the germination and the initial radicle growth of seeds of Raphanus sativus, Lactuca sativa and Lepidium sativum. The essential oils were obtained from Hyssopus officinalis, Lavandula angustifolia, Majorana hortensis, Melissa officinalis, Ocimum basilicum, Origanum vulgare, Salvia officinalis and Thymus vulgaris (Lamiaceae, Verbena officinalis (Verbenaceae, Pimpinella anisum, Foeniculum vulgare and Carum carvi (Apiaceae. The germination and radicle growth of tested seeds were affected in different ways by the oils. Thyme, balm, vervain and caraway essential oils were more active against both germination and radicle elongation.

  1. Studies on essential oils: part 10; antibacterial activity of volatile oils of some spices.

    Science.gov (United States)

    Singh, G; Kapoor, I P S; Pandey, S K; Singh, U K; Singh, R K

    2002-11-01

    The essential oils extracted from the seeds of seven spices, Anethum graveolens, Carum capticum, Coriandrum sativum, Cuminum cyminum, Foeniculum vulgare, Pimpinella anisum and Seseli indicum have been studied for antibacterial activity against eight pathogenic bacteria, causing infections in the human body. It has been found that the oil of C. capticum is very effective against all tested bacteria. The oil of C. cyminum and A. graveolens also gave similar results. These oils are equally or more effective when compared with standard antibiotics, at a very low concentration.

  2. Phytotoxic activities of Mediterranean essential oils.

    Science.gov (United States)

    de Almeida, Luiz Fernando Rolim; Frei, Fernando; Mancini, Emilia; De Martino, Laura; De Feo, Vincenzo

    2010-06-14

    Twelve essential oils from Mediterranean aromatic plants were tested for their phytotoxic activity, at different doses, against the germination and the initial radicle growth of seeds of Raphanus sativus, Lactuca sativa and Lepidium sativum. The essential oils were obtained from Hyssopus officinalis, Lavandula angustifolia, Majorana hortensis, Melissa officinalis, Ocimum basilicum, Origanum vulgare, Salvia officinalis and Thymus vulgaris (Lamiaceae), Verbena officinalis (Verbenaceae), Pimpinella anisum, Foeniculum vulgare and Carum carvi (Apiaceae). The germination and radicle growth of tested seeds were affected in different ways by the oils. Thyme, balm, vervain and caraway essential oils were more active against both germination and radicle elongation.

  3. AGASTACHE FOENICULUM – A PERSPECTIVE SOURCE OF MEDICAL PRODUCTS

    OpenAIRE

    V. V. Chumakova; O. I. Popova

    2013-01-01

    A phytochemical study has been conducted to explore Agastache foeniculum (Lamiaceae), which is widely used in oriental medicine for colds, inflammation of the gastrointestinal tract and urinary system; exter-nally for fungal dermatitis, seborrhea, hair growth and strengthening. In the course of the experiment methods of chemical (such as alkalimetry, permanganatometriya, chelatometry) and physico-chemical (such as mass spec-trometry coupled with GC, differential UV spectrophotometry, HPLC, pl...

  4. Condições de armazenamento e tempo de embebição na germinação de sementes de erva-doce (Pimpinella anisum L. Storage conditions and periods and imbibition times in anise (Pimpinella anisum L. seed germination

    Directory of Open Access Journals (Sweden)

    L.S.A.T. Takahashi

    2009-01-01

    Full Text Available O trabalho teve o objetivo de avaliar o efeito de diferentes condições e períodos de armazenamento e diferentes períodos de embebição na germinação de sementes de erva-doce. As sementes foram armazenadas por 1, 3, 5, 7 e 9 meses em temperatura ambiente (22 ± 2ºC e controlada (10ºC e em seguida foram submetidas a cinco períodos de embebição (0, 12, 24 e 36 horas. Os testes de germinação foram conduzidos em câmara de germinação à temperatura de 25ºC com fotoperíodo de 8 horas de luz e 16 horas de escuro. O delineamento experimental foi o inteiramente ao acaso com parcelas subdivididas com 4 repetições de 100 sementes. A porcentagem de germinação das sementes armazenadas em temperatura controlada foi maior que aquelas armazenadas em temperatura ambiente. As sementes armazenadas à 10ºC mantiveram uma porcentagem de germinação de 57,8% por até cinco meses, e não controlada, 67,0% por um mês. Períodos crescentes de embebição afetaram negativamente a porcentagem de germinação das sementes de erva-doce.This study aimed to evaluate the effect of different storage conditions and periods and different imbibition times in anise seed germination. Seeds were stored for 1, 3, 5, 7, and 9 months at room (22 ± 2ºC and controlled (10ºC temperatures and then subjected to five imbibition times (0, 12, 24, and 36 h. Germination tests were carried out in a germination chamber at 25ºC with photoperiod of 8 h light:16 h dark. The experimental design was completely randomized using split plot with 4 replicates of 100 seeds. Germination percentage was higher in seeds stored at controlled temperature than in those stored at room temperature. Seeds stored at 10ºC kept a germination percentage of 57.8% for up to five months, whereas those at uncontrolled temperature kept 67.0% for one month. Increasing imbibition periods negatively affected anise seed germination percentage.

  5. Screening of antioxidant properties of seven Umbelliferae fruits from Iran.

    Science.gov (United States)

    Nickavar, Bahman; Abolhasani, Farideh Al-Sadat

    2009-01-01

    Antioxidative activities (IC(50)) of ethanol extracts from seven Umbelliferae fruits (Bunium persicum, Coriandrum sativum, Cuminum cyminum, Foeniculum vulgare, Heracleum persicum, Pimpinella anisum and Trachyspermum copticum) have been studied by the DPPH (2,2'-diphenyl-1-picrylhydrazyl) radical scavenging test. All the studied extracts showed antioxidant capability and P. anisum extract exhibited the strongest activity. The scavenging activity of the extracts in decreasing order was: P. anisum> T. copticum> C. cyminum> F. vulgare > or = B. persicum > or = C. sativum> H. persicum. The extracts were also investigated regarding their total flavonoid contents by the AlCl3 technique. The decreasing order of the flavonoid content of the extracts was: C. cyminum > T. copticum> P. anisum > or = H. persicum > or = B. persicum > or = F. vulgare > or = C. sativum. However, a favorable correlation was not found between the antioxidant activity and the total flavonoid content of the extracts. As well, the most active extract (i.e. P. anisum) was partitioned with n-hexane, chloroform and ethyl acetate to yield three organic fractions together with the remaining aqueous fraction. The antioxidative activities (IP%) and flavonoid contents of the fractions were also determined. The ethyl acetate fraction exhibited the highest activity and content. A positive correlation was found between the antioxidant potency and flavonoid content of the fractions.

  6. Therapeutic and pharmacological potential of Foeniculum vulgare Mill: a review

    Directory of Open Access Journals (Sweden)

    Kooti Wesam

    2015-01-01

    Full Text Available Introduction: Fennel (Foeniculum vulgare Mill is one of the oldest spice plants which, due to its economic importance and significant pharmaceutical industry applications, is considered as one of the world’s most important medicinal plants. The purpose of this study is to investigate and collect scientific reports such as morphological characteristics, phytochemical compounds and evaluation of the therapeutic properties of this valuable medicinal plant that have been published. Methods: In order to gather the information the keywords Fennel and Foeniculum vulgare mill, therapeutic, and pharmacology have been searched until January 1, 2015 from journals accessible in databases such as ScienceDirect, Scopus, EBSCO, Medline, PubMed, Embase, SID and Iran Medex. Results: The results showed that this plant has various pharmacological properties including antioxidant, anti-cancer activity, anti-inflammatory, antifungal, anti-bacterial and estrogenic effects which are probably due to the presence of aromatic compounds such as anethole, estragole and fenshon. Conclusion: Fennel possesses various pharmacological properties and the fennel bioactive molecules play an important role in human health, hence, it might be used for different drug productions.

  7. Pharmacological evidence of hypotensive activity of Marrubium vulgare and Foeniculum vulgare in spontaneously hypertensive rat.

    Science.gov (United States)

    El Bardai, S; Lyoussi, B; Wibo, M; Morel, N

    2001-05-01

    The hypotensive effects of the water extract of Marrubium vulgare L. and Foeniculum vulgare L. were investigated in spontaneously hypertensive rats (SHR) and in normotensive Wistar-Kyoto rats (WKY). Oral administration of Marrubium or Foeniculum extract lowered the systolic blood pressure of SHR but not of WKY. In SHR, Foeniculum but not Marrubium treatment increased water, sodium and potassium excretion. Ex vivo as well as in vitro, Marrubium extract inhibited the contractile responses of rat aorta to noradrenaline and to KCl (100 mM). Inhibition was greater in aorta from SHR compared to WKY and was not affected by the NO synthase inhibitor N-nitro-L-arginine. Vascular effects of Foeniculum extract were less pronounced than those of Marrubium and were blocked by N-nitro-L-arginine. These results indicate that hypotensive activity of Marrubium and Foeniculum extracts seems to be mediated through different pathways: Foeniculum appeared to act mainly as a diuretic and a natriuretic while Marrubium displayed vascular relaxant activity.

  8. PENGARUH INFUS BUAH Foeniculum vulgare Mill PADA SIKLUS ESTRUS DAN BOBOT UTERUS TIKUS PUTIH

    Directory of Open Access Journals (Sweden)

    Sa'roni Sa'roni

    2012-10-01

    Full Text Available Buah Foeniculum vulgare Mill (adas secara tradisional untuk obat dan campuran jamu pengatur haid. Diduga buah Foeniculum vulgare Mill mempunyai pengaruh pada sistem reproduksi. Oleh karena itu, kami melakukan penelitian pengaruh infus buah Foeniculum vulgare Mill pada siklus estrus dan bobot uterus tikus putih.Infus diberikan secara oral sekali sehari selama 12 hari dengan dosis setara dengan 7,3 mg, 73 mg dan 219 mg/100 g bobot badan. Selama pemberian bahan, siklus estrus diperiksa setiap hari dan pada hari ke-13 diotopsi dan ditimbang bobot uterusnya.Hasil penelitian menunjukkan bahwa infus buah Foeniculum vulgare Mill setara dengan serbuk 7,3 mg, 73 mg dan 219 mg/100 g bobot badan dapat menghambat fase estrus (masa subur. Infus setara dengan serbuk 7 mg dan 219 mg/100 g bobot badan kelihatan dapat menghambat fase estrus yang sangat nyata dibandingkan dengan akuades. Infus buah Foeniculum vulgare Mill setara dengan serbuk 219 mg/100 g bobot badan dapat menurunkan bobot uterus, tetapi secara statistik tidak berbeda nyata dengan akuades. Kata kunci: Obat tradisional; Foeniculum vulgare Mill; Sistem Reproduksi

  9. Possible mechanism(s) for relaxant effects of Foeniculum vulgare on guinea pig tracheal chains.

    Science.gov (United States)

    Boskabady, M H; Khatami, A; Nazari, A

    2004-07-01

    In a previous study the relaxant (bronchodilatory) effect of Foeniculum vulgare on isolated guinea pig tracheal chains was demonstrated. To study mechanisms responsible for this effect the present study evaluated the inhibitory effect of this plant on contracted tracheal chains of guinea pig. The relaxant effects of aqueous and ethanol extracts and an essential oil from Foeniculum vulgare were compared to negative controls (saline for aqueous extract and essential oil and ethanol for ethanol extract) and a positive control (diltiazem) using isolated tracheal chains of the guinea pig precontracted by 10 microM methacholine (group 1) and 60 mM KCl (group 2, n = 7 for each group). In the group 1, experiments diltiazem, ethanol extract, and essential oil from Foeniculum vulgare showed a significant relaxant effect on methacholine induced contraction of tracheal chains compared to those of negative controls (p Foeniculum vulgare. However with regard to the effect of KCl on calcium channels, the results indicated that the inhibitory effect of ethanol extracts and essential oil from Foeniculum vulgare on calcium channels is not contributing to their relaxant (bronchodilatory) effects on guinea pig tracheal chains. However the results suggest a potassium channel opening effect for this plant, which may contribute on its relaxant effect on guinea pig tracheal chains.

  10. Actividade antimicrobiana do óleo essencial do Foeniculum vulgare Miller Antimicrobial activity of Foeniculum vulgare Miller essential oil

    Directory of Open Access Journals (Sweden)

    M. T. Tinoco

    2007-01-01

    Full Text Available O Foeniculum vulgare Mill. subsp. vulgare é uma planta espontânea da região mediterrânea, pertencente à família Apiaceae. O seu óleo essencial, principalmente o dos frutos secos, é amplamente utilizado nas indústrias alimentar, farmacêutica, cosmética e perfumaria. Neste trabalho, pretendeu-se avaliar a actividade antimicrobiana do óleo essencial dos frutos verdes e das folhas da variedade de funcho doce colhido no Alentejo, na região de Évora, e relacionar essa actividade com a respectiva composição química. A extracção dos óleos essenciais foi efectuada por hidrodestilação e a sua análise foi feita por GC-FID e GC-MS. No óleo das folhas foram identificados como componentes maioritários os fenilpropanóides anetol, fenchona e estragol e o monoterpeno a-felandreno, enquanto que o óleo dos frutos apresentou como constituintes predominantes anetol e fenchona. A actividade antimicrobiana dos óleos foi avaliada face às estirpes Staphylococcus aureus, Escherichia coli, Saccharomyces spp., Fusarium oxysporum e Penicillium sp.. Os óleos essenciais apresentaram actividade antimicrobiana contra S. aureus, Saccharomyces spp. e Fusarium oxysporum.Foeniculum vulgare Mill. ssp. vulgare is a spontaneous plant of Mediterranean region that belongs to the Apiaceae family. Its essential oil is used as additives in food, pharmaceutical, cosmetic and perfume industries mainly that one obtained from dried seeds. The main goal of this work were to evaluate the antimicrobial activity of essential oils obtained from fresh leaves and unripe seeds of sweet fennel, collected in Évora-Alentejo, in face of their chemical composition. The extraction of the essentials oils was made by hydrodistillation. Chemical analyses were carried out by GC-FID and GCMS. Phenylpropanoides anethole, fenchone, estragole and the monoterpene aphellandrene were the most abundant compounds of leaves essential oil. Seeds essential oil showed as main components

  11. Foeniculum vulgare essential oils: chemical composition, antioxidant and antimicrobial activities.

    Science.gov (United States)

    Miguel, Maria Graça; Cruz, Cláudia; Faleiro, Leonor; Simões, Mariana T F; Figueiredo, Ana Cristina; Barroso, José G; Pedro, Luis G

    2010-02-01

    The essential oils from Foeniculum vulgare commercial aerial parts and fruits were isolated by hydrodistillation, with different distillation times (30 min, 1 h, 2 h and 3 h), and analyzed by GC and GC-MS. The antioxidant ability was estimated using four distinct methods. Antibacterial activity was determined by the agar diffusion method. Remarkable differences, and worrying from the quality and safety point of view, were detected in the essential oils. trans-Anethole (31-36%), alpha-pinene (14-20%) and limonene (11-13%) were the main components of the essentials oil isolated from F. vulgare dried aerial parts, whereas methyl chavicol (= estragole) (79-88%) was dominant in the fruit oils. With the DPPH method the plant oils showed better antioxidant activity than the fruits oils. With the TBARS method and at higher concentrations, fennel essential oils showed a pro-oxidant activity. None of the oils showed a hydroxyl radical scavenging capacity > 50%, but they showed an ability to inhibit 5-lipoxygenase. The essential oils showed a very low antimicrobial activity. In general, the essential oils isolated during 2 h were as effective, from the biological activity point of view, as those isolated during 3 h.

  12. Phenolic glycosides from Foeniculum vulgare fruit and evaluation of antioxidative activity.

    Science.gov (United States)

    De Marino, Simona; Gala, Fulvio; Borbone, Nicola; Zollo, Franco; Vitalini, Sara; Visioli, Francesco; Iorizzi, Maria

    2007-07-01

    Two diglucoside stilbene trimers and a benzoisofuranone derivative were isolated from Foeniculum vulgare fruit together with nine known compounds. Their structures were elucidated by spectral methods including 1D, 2D NMR and MS and chemical methods. Antioxidant activity was tested using three methods: DPPH(), total antioxidant capacity and assay of lipid peroxidation.

  13. Foeniculum vulgare Mill: a review of its botany, phytochemistry, pharmacology, contemporary application, and toxicology.

    Science.gov (United States)

    Badgujar, Shamkant B; Patel, Vainav V; Bandivdekar, Atmaram H

    2014-01-01

    Foeniculum vulgare Mill commonly called fennel has been used in traditional medicine for a wide range of ailments related to digestive, endocrine, reproductive, and respiratory systems. Additionally, it is also used as a galactagogue agent for lactating mothers. The review aims to gather the fragmented information available in the literature regarding morphology, ethnomedicinal applications, phytochemistry, pharmacology, and toxicology of Foeniculum vulgare. It also compiles available scientific evidence for the ethnobotanical claims and to identify gaps required to be filled by future research. Findings based on their traditional uses and scientific evaluation indicates that Foeniculum vulgare remains to be the most widely used herbal plant. It has been used for more than forty types of disorders. Phytochemical studies have shown the presence of numerous valuable compounds, such as volatile compounds, flavonoids, phenolic compounds, fatty acids, and amino acids. Compiled data indicate their efficacy in several in vitro and in vivo pharmacological properties such as antimicrobial, antiviral, anti-inflammatory, antimutagenic, antinociceptive, antipyretic, antispasmodic, antithrombotic, apoptotic, cardiovascular, chemomodulatory, antitumor, hepatoprotective, hypoglycemic, hypolipidemic, and memory enhancing property. Foeniculum vulgare has emerged as a good source of traditional medicine and it provides a noteworthy basis in pharmaceutical biology for the development/formulation of new drugs and future clinical uses.

  14. Method for attaining fennel (Foeniculum vulgare Mill.) seed oil fractions with different composition and antioxidant capacity

    Science.gov (United States)

    Fennel (Foeniculum vulgare Mill.) is cultivated for its seeds and foliage, which contain essential oil. We hypothesized that the collection of fennel seed oil at different time points during the distillation process may result in fennel oil with distinct composition and bioactivity. We collected ess...

  15. Foeniculum vulgare Mill: A Review of Its Botany, Phytochemistry, Pharmacology, Contemporary Application, and Toxicology

    Directory of Open Access Journals (Sweden)

    Shamkant B. Badgujar

    2014-01-01

    Full Text Available Foeniculum vulgare Mill commonly called fennel has been used in traditional medicine for a wide range of ailments related to digestive, endocrine, reproductive, and respiratory systems. Additionally, it is also used as a galactagogue agent for lactating mothers. The review aims to gather the fragmented information available in the literature regarding morphology, ethnomedicinal applications, phytochemistry, pharmacology, and toxicology of Foeniculum vulgare. It also compiles available scientific evidence for the ethnobotanical claims and to identify gaps required to be filled by future research. Findings based on their traditional uses and scientific evaluation indicates that Foeniculum vulgare remains to be the most widely used herbal plant. It has been used for more than forty types of disorders. Phytochemical studies have shown the presence of numerous valuable compounds, such as volatile compounds, flavonoids, phenolic compounds, fatty acids, and amino acids. Compiled data indicate their efficacy in several in vitro and in vivo pharmacological properties such as antimicrobial, antiviral, anti-inflammatory, antimutagenic, antinociceptive, antipyretic, antispasmodic, antithrombotic, apoptotic, cardiovascular, chemomodulatory, antitumor, hepatoprotective, hypoglycemic, hypolipidemic, and memory enhancing property. Foeniculum vulgare has emerged as a good source of traditional medicine and it provides a noteworthy basis in pharmaceutical biology for the development/formulation of new drugs and future clinical uses.

  16. FOURIER TRANSFORM INFRA RED (FT-IR SPECTRAL STUDIES OF FOENICULUM VULGARE

    Directory of Open Access Journals (Sweden)

    V. Devika

    2013-03-01

    Full Text Available Plants have been used in traditional medicine for several thousand years. Medicinal plants as a group comprise approximately 8000 species and account for about 50% of all the higher flowering plant species in India. In the present study, the plant Foeniculum vulgare was subjected to FT-IR spectroscopy. FT-IR-is a vibrational spectroscopy that records absorptions of IR light by chemical bonds in all molecules incl. polymers. Foeniculum vulgare (Apiaceae commonly known as fennel is a well known and important medicinal and aromatic plant widely used as carminative, digestive, lactogogue and diuretic and in treating respiratory and gastrointestinal disorders. The results showed the detections of the bands in organic molecules. Thus the study became evident that the plant posses some bioactive compounds at various bands obtained after FT-IR.

  17. Repellent activity of constituents identified in Foeniculum vulgare fruit against Aedes aegypti (Diptera: Culicidae).

    Science.gov (United States)

    Kim, Do-Hyoung; Kim, Soon-Il; Chang, Kyu-Sik; Ahn, Young-Joon

    2002-11-20

    The repellent activity of materials derived from the methanol extract of fruits from Foeniculum vulgareagainst hungry Aedes aegypti females was examined using skin and patch tests and compared with that of the commercial N,N-diethyl-m-toluamide (deet) and (Z)-9-octadecenoic acid. The biologically active constituents of the Foeniculum fruits were characterized as (+)-fenchone and (E)-9-octadecenoic acid by spectroscopic analyses. Responses varied according to compound, dose, and exposure time. In a skin test with female mosquitoes, at a dose of 0.4 mg/cm(2), (+)-fenchone and (Z)-9-octadecenoic acid exhibited moderate repellent activity at 30 min after treatment, whereas deet provided >1 h of protection against adult mosquitoes at 0.2 mg/cm(2). (Z)-9-Octadecenoic acid was a more potent repellent agent than (E)-9-octadecenoic acid. (+)-Fenchone and (E)-9-octadecenoic acid merit further study as potential mosquito repellent agents or as lead compounds.

  18. Phytochemical, Antibacterial, Antifungal and Antioxidant Properties of Agastache foeniculum Essential Oil

    Directory of Open Access Journals (Sweden)

    Mohammad Hashemi

    2017-04-01

    Full Text Available Phytochemicals are good sources of natural antioxidants and have beneficial effects on human health. Many of phenolic phytochemicals have shown antimicrobial and antifungal activity. Anise hyssop (Agastache foeniculum [Pursh] Kuntze is a perennial aromatic plant of the Lamiaceae family with antimicrobial and antifungal properties and useful for gastrointestinal problems. In this investigation, the antioxidant activity of extracts, their antimicrobial activity against Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus, B. subtilis, Salmonella thyphimurium, S. enteritidis, Escherichia coli strains and their antifungal activity against Aspergillus niger and A. flavus was evaluated. Seven components were identified, representing 95.4% of the oils including methyl chavicol (83.1%, limonene (3.4%, spathulenol (3.1% and caryophyllene oxide (3.1%. Agar disk diffusion and broth micro-well dilution assays showed that B. subtilis was the most resistant strain against both of the EOs and E. coli was the most sensitive bacteria. Results of both disc diffusion and MIC showed that the EO was more effective against A. flavus than A. niger. Antioxidant activity of A. foeniculum by DPPH and ABTS assays revealed remarkable antioxidant activities of this EO comparing with BHT. Results of current study indicated that A. foeniculum EOs can be used as a food preservative in having antibacterial, antifungal and antioxidant activity for the control of food deterioration.

  19. Screening, Purification and Characterization of Anionic Antimicrobial Proteins from Foeniculum Vulgare.

    Science.gov (United States)

    Al Akeel, Raid; Mateen, Ayesha; Syed, Rabbani; Alyousef, Abdullah A; Shaik, Mohammed Rafi

    2017-04-08

    Foeniculum vulgare Mill., commonly called fennel, is a medicinal plant belonging to the Umbelliferae (Apiaceae) family, and is used in traditional medicine. Antibacterial peptides were isolated using sodium phosphate citrate buffer and, for extraction, cetyltrimethyl ammonium bromide (CTAB) buffer with pH 6, have been employed and antimicrobial activity tested against four reference strains. The extracted protein was subjected to 3 kDa dialysis and separation was carried out by DEAE-ion exchange chromatography and further proteins were identified by 2D gel electrophoresis. The results of Foeniculum vulgare elutes obtained from DEAE-ion exchange chromatography were tested for antibacterial activity. Elute 3 shows the highest antibacterial activity on Pseudomonas aeruginosa with a diameter of a zone of inhibition of 16 mm and IC50 value 25.02 (mcg/mL). Based on the findings of the wide usage in treatment of various ailments and day-to-day life, Foeniculum vulgare seeds were used in the present research and have shown promising antibacterial effects, which requires further proteomic research to authenticate the role of the anticipated proteins.

  20. Chemistry, antioxidant, antibacterial and antifungal activities of volatile oils and their components.

    Science.gov (United States)

    De Martino, Laura; De Feo, Vincenzo; Fratianni, Florinda; Nazzaro, Filomena

    2009-12-01

    The present paper reports the chemical composition, antioxidant and antibacterial activities of several essential oils and their components. Analysis showed that three oils (Carum carvi L., Verbena officinalis L. and Majorana hortensis L.) contained predominantly oxygenated monoterpenes, while others studied (Pimpinella anisum L., Foeniculum vulgare Mill.) mainly contained anethole. C. carvi, V. officinalis and M. hortensis oils exhibited the most potent antioxidant activity, due their contents of carvacrol, anethole and estragol. Antibacterial action was assessed against a range of pathogenic and useful bacteria and fungi of agro-food interest. V. officinalis and C. carvi oils proved the most effective, in particular against Bacillus cereus and Pseudomonas aeruginosa. Carvacrol proved most active against Escherichia coli, and completely inhibited the growth of Penicillium citrinum. The oils proved inactive towards some Lactobacilli strains, whereas single components showed an appreciable activity. These results may be important for use of the essential oils as natural preservatives for food products.

  1. Production of Low Calorie Bakery Product with Pleasant Flavour, Antioxidant and Antimicrobial Activities

    Directory of Open Access Journals (Sweden)

    Hussein Ahmed M.S.

    2014-12-01

    Full Text Available Aromatic plants are considered sources of antioxidants, antimicrobial and favouring agents. Four aromatic plants (Thymus vulgaris L., Foeniculum vulgare, Pimpinella anisum L. and Trigonellafoenum-graecum L. were analysed in the study. Yoghurt was used to produce a low calorie pie. Chemical and rheological parameters, baking performance, staling rate and sensory properties of the pie were investigated. Volatile aroma compounds were anal-ysed with GC and GC/ MS, and antioxidant activity was evaluated by DPPH and β-carotene assays. The incorporation of yoghurt and some aromatic plants in the pie improved protein, fat, fibre, ash, and minerals contents and allowed achieving about 19% reduction in calories. Sensory evaluation of pie containing the mixture of aromatic plants showed its superior sensory quality. In addition, it could be concluded that aromatic plants were able to inhibit the growth of yeast, mould and bacteria and to prolong the storage periods of pie compared with the control.

  2. Local Anesthetic Activity from Extracts, Fractions and Pure Compounds from the Roots of Ottonia anisum Spreng. (Piperaceae).

    Science.gov (United States)

    López, Kelvin S E; Marques, André M; Moreira, Davyson DE L; Velozo, Leosvaldo S; Sudo, Roberto T; Zapata-Sudo, Gisele; Guimarães, Elsie F; Kaplan, Maria Auxiliadora C

    2016-01-01

    Piperaceae species can be found worldwide in tropical and subtropical areas and many of them have been used for centuries in traditional folk medicine and in culinary. In Brazil, species of Piperaceae are commonly used in some communities as local anesthetic and analgesic. Countrified communities have known some species of the genus Ottonia as "anestesia" and it is a common habit of chewing leaves and roots of Ottonia species to relief toothache. The purpose of this study is to report our findings on new molecules entities obtained from the roots of Ottonia anisum Spreng, in which local anesthetic activity (sensory blockage) is demonstrated for the first time in vivo guinea pig model. Phytochemical investigation led to the isolation of three amides (pipercallosidine, piperine and valeramide) and in an enriched mixture of seven amides (valeramide, 4,5-dihydropiperlonguminine, N-isobutil-6-piperonil-2-hexenamide, piperovatine, dihydropipercallosidine, pipercallosidine and pipercallpsine). Our findings demonstrated the anesthetic potential for the methanolic extract from roots, its n-hexane partition and amides from O. anisum and it is in agreement with ethnobotanical survey.

  3. Local Anesthetic Activity from Extracts, Fractions and Pure Compounds from the Roots of Ottonia anisum Spreng. (Piperaceae

    Directory of Open Access Journals (Sweden)

    KELVIN S.E. LÓPEZ

    Full Text Available ABSTRACT Piperaceae species can be found worldwide in tropical and subtropical areas and many of them have been used for centuries in traditional folk medicine and in culinary. In Brazil, species of Piperaceae are commonly used in some communities as local anesthetic and analgesic. Countrified communities have known some species of the genus Ottonia as "anestesia" and it is a common habit of chewing leaves and roots of Ottonia species to relief toothache. The purpose of this study is to report our findings on new molecules entities obtained from the roots of Ottonia anisum Spreng, in which local anesthetic activity (sensory blockage is demonstrated for the first time in vivo guinea pig model. Phytochemical investigation led to the isolation of three amides (pipercallosidine, piperine and valeramide and in an enriched mixture of seven amides (valeramide, 4,5-dihydropiperlonguminine, N-isobutil-6-piperonil-2-hexenamide, piperovatine, dihydropipercallosidine, pipercallosidine and pipercallpsine. Our findings demonstrated the anesthetic potential for the methanolic extract from roots, its n-hexane partition and amides from O. anisum and it is in agreement with ethnobotanical survey.

  4. Screening of Potential Free Radicals Scavenger and Antibacterial Activities of Purwoceng (Pimpinella alpina Molk)

    Science.gov (United States)

    Wahyuningrum, Retno; Utami, Pri Iswati; Dhiani, Binar Asrining; Kumalasari, Malikhah; Kusumawardani, Rizka Sari

    2016-01-01

    Purwoceng (Pimpinella alpina Molk) is a traditional medicinal plant used for its aphrodisiac values. This plant was originated Dieng Plateu, Central Java, Indonesia. Purwoceng has been reported to contain steroid, flavonoids, glycoside, saponins, tannins, and phenolic. Based on secondary metabolite compounds of Purwoceng herbs, a research need to be done to determine the other potential free radicals scavenger and antibacterial activities of Purwoceng. The objectives of this research are to screen the potential free radicals scavenger activity of in vitro using DPPH (1,1 diphenyl-2-picryl-hydrazil) radicals and NO• (nitric oxide) radicals, and antibacterial activity of Purwoceng. The extraction is done by a maceration method with petroleum ether, ethyl acetate, and ethanol solvent, respectively. Free radicals scavenger test was performed using DPPH radicals and NO• radicals, while antibacterial activity screening was performed using agar diffusion test. The results showed that ethyl acetate extract of Purwoceng has free radical scavenger activity with IC50 53.07 ppm lower than butylated hydroxytoluene. Ethyl acetate extract and ethanol extract of Purwoceng have antibacterial activity against Staphyloccus aureus, Escherichia coli, and MG42 bacterial isolate. PMID:27965755

  5. Screening of Potential Free Radicals Scavenger and Antibacterial Activities of Purwoceng (Pimpinella alpina Molk).

    Science.gov (United States)

    Wahyuningrum, Retno; Utami, Pri Iswati; Dhiani, Binar Asrining; Kumalasari, Malikhah; Kusumawardani, Rizka Sari

    2016-11-01

    Purwoceng (Pimpinella alpina Molk) is a traditional medicinal plant used for its aphrodisiac values. This plant was originated Dieng Plateu, Central Java, Indonesia. Purwoceng has been reported to contain steroid, flavonoids, glycoside, saponins, tannins, and phenolic. Based on secondary metabolite compounds of Purwoceng herbs, a research need to be done to determine the other potential free radicals scavenger and antibacterial activities of Purwoceng. The objectives of this research are to screen the potential free radicals scavenger activity of in vitro using DPPH (1,1 diphenyl-2-picryl-hydrazil) radicals and NO• (nitric oxide) radicals, and antibacterial activity of Purwoceng. The extraction is done by a maceration method with petroleum ether, ethyl acetate, and ethanol solvent, respectively. Free radicals scavenger test was performed using DPPH radicals and NO• radicals, while antibacterial activity screening was performed using agar diffusion test. The results showed that ethyl acetate extract of Purwoceng has free radical scavenger activity with IC50 53.07 ppm lower than butylated hydroxytoluene. Ethyl acetate extract and ethanol extract of Purwoceng have antibacterial activity against Staphyloccus aureus, Escherichia coli, and MG42 bacterial isolate.

  6. The Effect of Citrus Aurantium, Foeniculum Vulgare and Rosmarinus Officinalis Essential Oils on Peroxidase Activity

    Directory of Open Access Journals (Sweden)

    Maryam Mohajerani (PhD

    2016-02-01

    Full Text Available Background and objective: Peroxidases catalyze protein oxidation and lipid peroxidation. The activity of these enzymes in nerve cells is involved in causing disorders such as Alzheimer's and Parkinson's disease. This study investigated the effect of Citrus aurantium, Foeniculum vulgare and Rosmarinus officinalis essential oils on activity of peroxidase enzyme. Methods: All three medicinal plants were dried at room temperature. Their essential oil was extracted by steam distillation using a Clevenger apparatus. Optimal reaction conditions were determined in the presence of hydrogen peroxide and guaiacol as substrate and hydrogen donor, respectively. Enzyme kinetics of zucchini peroxidase were evaluated by increasing the amount of essential oils in optimal reaction conditions. Enzyme reaction rate for each of the essential oils and the Km and Vmax values were determined. Results: The results indicated concentration-dependent effect of the extracted essential oils on enzyme kinetics at optimum temperature of 50 °C and optimal pH of 6.5. The essential oil of Citrus aurantium had non-competitive inhibitory effects on the enzyme with Km of 6.25 mM, while the enzyme’s Vmax significantly reduced by increasing the concentration. Foeniculum vulgare showed mixed inhibition effect with Km of 7.14 mmol per 20 μl of the essential oil, but had a decreasing effect on the Vmax in smaller amounts. Finally, Rosmarinus officinalis showed activating effects by reducing the Km to 4-5.88 mM. Conclusion: The essential oils of Citrus aurantium and Foeniculum vulgare are inhibitors of the peroxidase enzyme and can be further studied as natural herbal medicines.

  7. Influences of various factors on hairy root induction in Agastache foeniculum (Pursh Kuntze

    Directory of Open Access Journals (Sweden)

    Elnaz NOUROZI

    2016-04-01

    Full Text Available Agrobacterium rhizogenes is known as a natural tool of genetic engineering in many plant species. For the first time, hairy root induction in Agastache foeniculum using A. rhizogenes, rosmarinic acid content and the effect of different culture media and inoculation methods on hairy root growth rate were investigated. Hairy root culture of A. foeniculum was established by inoculation of the 1-month-old leaf explant with A4 strain of A. rhizogenes and the effectiveness of light – dark conditions and two inoculation methods (immersion and injection were tested. Furthermore, in immersion method, the effects of inoculation time (3, 5 and 7 min on root induction were investigated. In the second part of the study, the hairy root culture of A. foeniculum was studied using different basal culture media (MS, 1/2 MS and B5. Rosmarinic acid content in hairy roots and non- transformed roots was analyzed using high-performance liquid chromatography (HPLC. There was no significant difference between various inoculation methods in the ability of hairy roots induction. Observations showed that percentage of hairy root induction was higher when the explants were immersed for 5 min in bacterial suspension. Light conditions displayed the highest hairy root induction rates compared with dark condition. Various culture media are different in terms of types and amounts of nutrients and have influence on growth rate. The maximum growth rate (1.61 g fr wt/50 ml of hairy roots were obtained in 1/2 MS medium. Rosmarinic acid content in transformed roots (213.42 µg/g dry wt was significantly higher than non-transformed roots (52.28 µg/ g dry wt.

  8. Essential oil composition and antifungal activity of Foeniculum vulgare Mill obtained by different distillation conditions.

    Science.gov (United States)

    Mimica-Dukić, N; Kujundzić, S; Soković, M; Couladis, M

    2003-04-01

    The influence of different hydrodistillation conditions was evaluated from the standpoint of essential oil yield, chemical composition and antifungal activity from seeds of Foeniculum vulgare Mill. Three hydrodistillation conditions were considered. The main constituents of the oils were: (E)-anethole (72.27%-74.18%), fenchone (11.32%-16.35%) and methyl chavicol (3.78%-5.29%). The method of distillation significantly effected the essential oil yield and quantitative composition, although the antifungal activity of the oils against some fungi was only slightly altered.

  9. A striking and frequent cause of premature thelarche in children: Foeniculum vulgare.

    Science.gov (United States)

    Türkyilmaz, Zafer; Karabulut, Ramazan; Sönmez, Kaan; Can Başaklar, Abdullah

    2008-11-01

    Isolated premature thelarche is a common disorder characterized by breast development, usually younger than 2 years, with no other signs of puberty. Although it is usually associated with adrenal or ovarian disorders, hypothyroidism, and use of exogenous hormones or drugs, it may also be associated with long-term use of herbal medicine. Thus, long-term use of preparations such as Foeniculum vulgare, which is used to eliminate gas and regulate intestinal function in children, may cause premature thelarche, and thus, the use of such preparations should be limited.

  10. Antiinflammatory, analgesic and antioxidant activities of the fruit of Foeniculum vulgare.

    Science.gov (United States)

    Choi, Eun-Mi; Hwang, Jae-Kwan

    2004-09-01

    Oral administration (200 mg/kg) of Foeniculum vulgare fruit methanolic extract exhibited inhibitory effects against acute and subacute inflammatory diseases and type IV allergic reactions and showed a central analgesic effect. Moreover, it significantly increased the plasma superoxide dismutase (SOD) and catalase activities and the high density lipoprotein-cholesterol level. On the contrary, the malondialdehyde (MDA) (as a measure of lipid peroxidation) level was significantly decreased in F. vulgare fruit methanolic extract group compared to the control group (P<0.05). These results seems to support the use of F. vulgare fruit methanolic extract in relieving inflammation.

  11. [Spectral analysis of elements in different samples of processed Foeniculum vulgare].

    Science.gov (United States)

    Zhang, Fan; Li, Zhen; Hamulati, Wufuer; Tian, Shu-Ge

    2010-03-01

    The compositions and contents of chemical compounds in traditional Chinese medicines would be changed after being processed using different methods, and their pharmacological activity may be influenced. ICP-AES, AAS and AFS were the first methods to be used to scan and analyze macro elements, trace elements and heavy metals in Foeniculum vulgare and its nine different processed samples in the present paper, which were correlated with people's health and lives. The experiment results showed that the three kinds of analyzing methods could be used to judge the contents and the changing trend of all elements in traditional Chinese medicine quickly and truly. Thirty two elements were found in F. vulgare, and there were noticeable changes in the contents of some elements in processed samples compared with F. vulgare without processing. These results indicated that there is great relevance between changes in the contends of elements with different processing methods, and auxiliary materials can not only change the contents of elements but also play a role in treating ills with effective constituents. Also, it was firstly found that the content of Hg rose greatly in the experiment, and these showed there was notable potential safety hazard when processed Foeniculum vulgare was used. These experiments widen the application of spectrum analyzing methods in safety evaluation of traditional Chinese medicine.

  12. Acaricidal activity of constituents identified in Foeniculum vulgare fruit oil against Dermatophagoides spp. (Acari: Pyroglyphidae).

    Science.gov (United States)

    Lee, Hoi-Seon

    2004-05-19

    Acaricidal activities of components derived from Foeniculum vulgare fruit oil against Dermatophagoides farinae and Dermatophagoides pteronyssinus were examined using direct contact application and compared with that of the commercial repellent benzyl benzoate. The major biologically active constituent of Foeniculum fruit oil was characterized as (+)-fenchone by spectroscopic analyses. On the basis of LD(50) values, the compound most toxic to D. farinae was p-anisaldehyde (11.3 mg/m(2)) followed by (+)-fenchone (38.9 mg/m(2)), (-)-fenchone (41.8 mg/m(2)), benzyl benzoate (89.2 mg/m(2)), thymol (90.3 mg/m(2)), and estragol (413.3 mg/m(2)). Against D. pteronyssinus, p-anisaldehyde (10.1 mg/m(2)) was much more effective than benzyl benzoate (67.5 mg/m(2)), thymol (68.5 mg/m(2)), and estragol (389.9 mg/m(2)). These results indicate that the acaricidal activity of F. vulgare fruit oil likely results from (+)-fenchone and p-anisaldehyde. (+)-Fenchone was 20.3 times more abundant in the oil than p-anisaldehyde. (+)-Fenchone and p-anisaldehyde merit further study as potential house dust mite control agents or as lead compounds.

  13. Evaluation of Salt Stress at Gerimination and Seedling Stages of Foeniculum vulgare Mill. in Hydroponic Condition

    Directory of Open Access Journals (Sweden)

    2015-11-01

    Full Text Available Identification of medicinal plants with tolerance to salinity stress has an economical importance in Iran. This study was carried out to evaluate the effects of salinity stress on different Morpho-Physiological traits of Foeniculum vulgare Mill in germination and seedling stage in hydroponic condition. The experiment was conducted as a split plot based on a Randomized Complete Block Design (RCBD with four (in first experiment and three (in second experiment replications. Salinity effects of NaCl [0, 50, 100, 150 200 (Mm] was evaluated on three genotypes of  Foeniculum vulgare (Estahban, Isfahan, Shiraz. Analysis of variance showed that salinity levels reduced the percentage of germination, seed vigor index, rootlenght, stem length, root dry weight, shoot dry weight, biomass, the ratio of shoot to root, chlorophyll index, chlorophyll a and b and caroteniod content. Significant variation was observed between different genotypes on different levels of salinity. Tolerance to salinity in seedling was more than germination. In two experiments, the genotype of Shiraz showed superiority in view point of tolerance.

  14. Larvicidal activity of Ottonia anisum metabolites against Aedes aegypti: A potential natural alternative source for mosquito vector control in Brazil.

    Science.gov (United States)

    Marques, André M; Velozo, Leosvaldo S; Carvalho, Michelle A; Serdeiro, Michele T; Honório, Nildimar A; Kaplan, Maria Auxiliadora C; Maleck, Marise

    2017-01-01

    Aedes aegypti mosquito is the principal vector of the viruses responsible for urban yellow fever, dengue, dengue haemorrhagic fever, as well as Zika and chikungunya in Brazil. The present study was aimed to investigate the insecticidal potential of the extract and fractions of Ottonia anisum, along with special metabolites isolated from it, as natural alternatives against larvae (L3) of Ae. aegypti, vector of potentially deadly tropical infections in Brazil. The plant species O. anisum was collected in March 2015, at Xerιm area, in Rio de Janeiro City, Brazil. Crude extracts and the isolated pure compounds were screened for toxicity against Ae. aegypti larvae (L3). Bioassays were performed on 20 larvae (L3) of Ae. aegypti in triplicate. The samples were dissolved in a mixture of acetone and DMSO at final concentrations of 1-200 μg/ml. The toxicity of the solutions was evaluated towards the growth and development of Ae. aegypti larvae till emergence of adults. The crude hexane extract showed 100% larval mortality 24 h after treatment at a concentration of 200 μg/ml. The bioassays using 1-butyl-3,4-methylenedioxybenzene revealed 100% mortality among L3 larvae, 24 h afterthe treatment at a concentration of 30 μg/ml, the LC recorded was 1.6 μg/ml. At concentration of 10 μg/ml, the L3 larval mortality recorded was 92%. The metabolite 1-butyl-3,4-methylenedioxybenzene showed potent toxicity against Ae. aegypti larvae. This arylbutanoid agent could be used as a natural alternative adjuvant pesticide, in new compositions that would be environmentally safer.

  15. PHENOLIC PROFILE, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES FROM THE Apiaceae FAMILY (DRY SEEDS

    Directory of Open Access Journals (Sweden)

    Valentina Lubomirova Christova-Bagdassarian

    2013-11-01

    Full Text Available Objective: In the present study, a comparative evaluation of the phenolic compounds antioxidant capacity and antibacterial activity to several kinds of seeds from the Apiacea family, to which belong: Foeniculum vulgare (Fennel, Anethum graveolens (Dill, Pimpinella anisum (Anise, Carum carvi (Caraway and Coriandrum sativum (Coriander were carried out. Methods: The total phenolic content of seeds was measured spectrophotometrically by using the Folin-Ciocalteu assay, the total flavonoids was measured spectrophotometrically by using the aluminum chloride colorimetric. Antioxidant capacity was analysed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH radical scavenging effect of the seeds and was determined also spectrophotometrically. Antibacterial activity was analysed by ISO standards. Results: The total phenolic and flavonoid contents of the Foeniculum vulgare (Fennel varied between 115.96 mg GAE/100g and 68.10 mg CE/100g .The Coriandrum sativum (Coriander content is lower (from 17.04 mg GAE/100g to 11.10 mg CE/100g, respecively. The highest radical scavanging effect was observed in the Foeniculum vulgare (Fennel with IC50 of 113.19 ml/L. In our study, the methanol extract of seeds didn’t have any antimicrobial activity. Conclusions: An original data for total phenolic and total flavonoid contents are present in this study. They are a basis for assessment of the role of Apiaceae family dry seeds against free radicals effect and antibacterial activity. The results show that methanolic extract has the highest of total phenolic and total flavonoid contents, high potential of antioxidant activity of dry seeds from Apiaceae family, to which belong: Fennel (Foeniculum vulgare, Dill (Anethum graveolens, Anise (Pimpinella anisum, Caraway (Carum carvi and Coriander (Coriandrum sativum. From all results, the solvent extracts of dry seeds are rich in phytochemical contents, which possessed high antioxidant and antimicrobial activities. Therefore the data found in

  16. AKTIVITAS ANTIBAKTERI EKSTRAK BUAH ADAS (Foeniculum vulgare, Mill PADA Vibrio harveyi DAN Vibrio alginolyticus Antibacterial Activity of Fennel (Foeniculum vulgare Mill Extract on Vibrio alginolyticus and Vibrio harveyi

    Directory of Open Access Journals (Sweden)

    Budianto Budianto

    2015-10-01

    Pada penelitian ini menggunakan ekstrak air dari buah adas untuk mengetahui aktivitas antibakteri terhadap Vibrio harveyi dan Vibrio alginolyticus dengan menggunakan metode uji Minimum Inhibitory Concentration (MIC dan difusi cakram kertas. Hasil yang diperoleh pada uji MIC, konsentrasi terkecil untuk menghambat pertumbuhan adalah 0,060 g/ml, untuk kedua spesies bakteri. Variasi perlakuan pada uji cakram kertas yaitu konsentrasi A (0,065 g/ml, B (0,070 g/ml, C (0,075 g/ml, D (0,080 g/ml, E (0,085 g/ml, F (0,090 g/ml dan kontrol (0,000 g/ml, hasil yang diperoleh adalah konsentrasi 0,090 g/ml memiliki diameter zona hambat tertinggi sebesar 11,17 ± 0,5 mm (V. harveyi dan 12,53 ± 1,14 mm (V. alginolyticus, sehingga dapat disimpulkan bahwa buah adas (F. vulgare Mill memiliki peranan ekologi yang sangat penting sebagai bahan pengobatan alternatif dalam pengendalian penyebaran penyakit Vibriosis yang disebabkan oleh V. harveyi dan V. alginolyticus. Kata kunci: Foeniculum vulgare Mill, Vibrio harveyi, Vibrio alginolyticus, uji MIC dan difusi cakram kertas

  17. Cholinergic basis of memory-strengthening effect of Foeniculum vulgare Linn.

    Science.gov (United States)

    Joshi, Hanumanthachar; Parle, Milind

    2006-01-01

    Alzheimer's disease is a neurodegenerative disorder associated with a decline in cognitive abilities. Dementia is one of the age-related mental problems and a characteristic symptom of Alzheimer's disease. Nootropic agents are used in situations where there is organic disorder in learning abilities. The present work was undertaken to assess the potential of Foeniculum vulgare Linn. extract as a nootropic and anticholinesterase agent in mice. Methanolic extract of the whole plant of F. vulgare Linn. administered for eight successive days ameliorated the amnesic effect of scopolamine (0.4 mg/kg) and aging- induced memory deficits in mice. The passive avoidance paradigm served as the exteroceptive behavioral model for assessing memory. F. vulgare extract increased step-down latency and acetylcholinesterase inhibition in mice significantly. Hence, F. vulgare can be employed in treatment of cognitive disorders such as dementia and Alzheimer's disease.

  18. Antihirsutism activity of Fennel (fruits of Foeniculum vulgare) extract. A double-blind placebo controlled study.

    Science.gov (United States)

    Javidnia, K; Dastgheib, L; Mohammadi Samani, S; Nasiri, A

    2003-01-01

    Idiopathic hirsutism is defined as the occurrence of excessive male pattern hair growth in women who have a normal ovulatory menstrual cycle and normal levels of serum androgens. It may be a disorder of peripheral androgen metabolism. In this study we evaluated the clinical response of idiopathic hirsutism to topical Fennel extract. Fennel, Foeniculum vulgare, is a plant, which has been used as an estrogenic agent. The ethanolic extract of Fennel was obtained by using a soxhlete apparatus. In a double blind study, 38 patients were treated with creams containing 1%, 2% of Fennel extract and placebo. Hair diameter was measured and rate of growth was considered. The efficacy of treatment with the cream containing 2% Fennel is better than the cream containing 1% Fennel and these two were more potent than placebo. The mean values of hair diameter reduction was 7.8%, 18.3% and -0.5% for patients receiving the creams containing 1%, 2% and 0% (placebo) respectively.

  19. Antibacterial activity of Coriandrum sativum L. and Foeniculum vulgare Miller Var. vulgare (Miller) essential oils.

    Science.gov (United States)

    Lo Cantore, Pietro; Iacobellis, Nicola S; De Marco, Adriana; Capasso, Francesco; Senatore, Felice

    2004-12-29

    Essential oils were extracted from the fruits of Coriandrum sativum L. and Foeniculum vulgare Miller var. vulgare (Miller) and assayed in vitro for antibacterial activity to Escherichia coli and Bacillus megaterium, bacteria routinely used for comparison in the antimicrobial assays, and 27 phytopathogenic bacterial species and two mycopathogenic ones responsible for cultivated mushroom diseases. A significant antibacterial activity, as determined with the agar diffusion method, was shown by C. sativum essential oil whereas a much reduced effect was observed for F. vulgare var. vulgare oil. C. sativum and F. vulgare var. vulgare essential oils may be useful natural bactericides for the control of bacterial diseases of plants and for seed treatment, in particular, in organic agriculture. The significant antibacterial activity of essential oils to the bacterial pathogens of mushrooms appears promising.

  20. Depolymerized carrageenan ameliorates growth, physiological attributes, essential oil yield and active constituents of Foeniculum vulgare Mill.

    Science.gov (United States)

    Hashmi, Nadeem; Khan, M Masroor A; Moinuddin; Idrees, Mohd; Khan, Zeba H; Ali, Akbar; Varshney, Lalit

    2012-09-01

    Irradiated carrageenan (IC) elicits an array of plant defense responses and biological activities in plants. An experiment was carried out in the naturally illuminated conditions of net house in order to assess the effects of foliar spray of IC on agricultural performance of fennel (Foeniculum vulgare Mill.), which is a high-value essential oil bearing medicinal crop used in pharmaceutical, food and cosmetic industries. There were applied four IC concentrations (40, 60, 80 and 100 mg L(-1)) as foliar sprays. Application of IC significantly improved the growth attributes, physiological and biochemical parameters, essential oil yield and the contents of main components of essential oil of fennel. IC applied at 80 mg L(-1) enhanced these parameters maximally. Unirradiated carrageenan and deionized water had no effect on the attributes studied. Moreover, GLC analysis revealed a significant increase in the components of essential oil, viz. fenchone (4.48-7.82%) and anethole (78.38-86.08%) compared to the control.

  1. An Acylated Kaempferol Glycoside from Flowers of Foeniculum vulgare and F. Dulce

    Directory of Open Access Journals (Sweden)

    Shahera M. Ezzat

    2002-02-01

    Full Text Available An acylated kaempferol glycoside, namely kaempferol-3-O-α-L-(2”,3”-di-E-pcoumaroyl-rhamnoside (1 was isolated from the flowers of Foeniculum vulgare Mill. and F. dulce DC. It is thus isolated for the first time from family Apiaceae. In addition, the different organs of both plants afforded six flavonoid glycosides - namely afzelin (kaempferol-3-O-α-L-rhamnoside (2, quercitrin (3, isorhamnetin-3-O-β-D-glucoside (4, isoquercitrin (5, rutin (6, and miquelianin (quercetin-3-O-β-D-glucuronide (7. Structure elucidation of the above mentioned flavonoids was achieved by UV, 1H- and 13C-NMR, 1H-1H COSY, HMQC and EI-MS.

  2. Sodium Chloride-Induced leaf senescence in Hydrocotyle bonariensis Lam. and Foeniculum vulgare L.

    Directory of Open Access Journals (Sweden)

    Claudia R. B. Haddad

    1999-01-01

    Full Text Available Leaf senescence induced by sodium chloride was studied in Hydrocotyle bonariensis Lam. and Foeniculum vulgare L. Both species belong to Umbelliferae family, however only H. bonariensis grows spontaneously in sandy soils of coastal regions (saline soils. Leaves of plants receiving nutrient solutions containing different concentrations of NaCl were evaluated for fresh and dry weights and chlorophyll content. The denaturating electrophoretic profiles of leaf proteins were also studied. Sodium chloride changed the protein profile of F. vulgare and hastened the leaf senescence of both the species. However, plants of H. bonariensis receiving 599 mM NaCl lasted longer than F. vulgare. Therefore, the occurrence of H. bonariensis in saline soils might be related with mechanisms of salinity tolerance.Foi estudada a senescência de folhas, induzida por cloreto de sódio, em Hidrocotyle bonariensis Lam. e Foeniculum vulgare L. Ambas as espécies pertencem à família Umbellifera. Contudo, somente H. bonariensis cresce espontaneamente em solos arenosos da região costeira (solos salinos. Foram verificadas a massa fresa, massa seca e concentração de clorofila de folhas de plantas recebendo soluções de NaCl com diferentes concentrações. O perfil eletroforético denaturante de proteínas das folhas também foi avaliado. Aplicação de cloreto de sódio levou a alterações no perfil protéico de F. vulgare e acelerou a senescência das folhas das duas espécies. Todavia, plantas de H. bonariensis recebendo soluções de NaCl com 599 mM sobreviveram mais tempo que as de F. vulgare. Assim a ocorrência de H. bonariensis em solos salinos pode estar relacionada a mecanismos de tolerância à salinidade.

  3. Headspace solvent microextraction-gas chromatography-mass spectrometry for the analysis of volatile compounds from Foeniculum vulgare Mill.

    Science.gov (United States)

    Fang, Lianghua; Qi, Meiling; Li, Ting; Shao, Qinglong; Fu, Ruonong

    2006-06-07

    A novel and rapid headspace solvent microextraction followed by gas chromatography-mass spectrometry (HSME-GC-MS) for the analysis of the volatile compounds of Foeniculum vulgare Mill is described. HSME parameters including extracting solvent, extraction temperature and time, headspace volume and particle size were optimized. As a result, benzyl alcohol was finally used for the extraction at 70 degrees C for 20 min with headspace volume of 12.1 ml and particle size of 120 mesh. Under the determined conditions, the powered samples of Foeniculum vulgare Mill were directly applied for the analysis. A comparison of HSME-GC-MS, solid phase microextraction (SPME)-GC-MS and steam distillation (SD)-GC-MS methods was made and showed that the HSME-GC-MS method was simple, inexpensive and effective and can be used for the analysis of volatile compounds in traditional Chinese medicines (TCMs).

  4. Estrogenic activity of isolated compounds and essential oils of Pimpinella species from Turkey, evaluated using a recombinant yeast screen.

    Science.gov (United States)

    Tabanca, Nurhayat; Khan, Shabana I; Bedir, Erdal; Annavarapu, Srinivas; Willett, Kristine; Khan, Ikhlas A; Kirimer, Nese; Baser, K Husnu Can

    2004-08-01

    Several plants and plant-derived pure compounds, designated as phytoestrogens, have been reported to cause estrogenic effects. They have been used for alleviation of menopausal symptoms, prevention of osteoporosis, heart disease and cancer. There is an increased interest in studying phytoestrogens such as isoflavones and lignans for their use as replacements for synthetic estrogens. In this study, the estrogenic activity of essential oils of eleven Pimpinella species and the compounds isolated from these species were evaluated using the yeast estrogen screen (YES) assay. The essential oils containing (E)-anethole as major compound showed estrogenic activity in the YES assay, except for the aerial parts without fruits of P. anisetum and P. flabellifolia. The percent maximal response produced by most anethole-containing oils was 30-50%. Fruits of P. isaurica and P. peucedanifolia were estrogenic in spite of the absence or trace amount of anethole, respectively. This study indicates that the estrogenic activity of Pimpinella oils is not solely due to the presence of anethole. Components other than anethole may be responsible for contributing towards the estrogenic activity. The essential oils from different species varied in their estrogenic potencies (relative potency from 8.3 x 10(-8) to 1.2 x 10(-6) compared to 17 beta-estradiol) and among the different plant parts, the fruit oils of most species were estrogenic followed by the aerial parts without fruits and the root oils and their EC50 values varied from 45 micrograms/mL to 650 micrograms/mL.

  5. Fatty acid composition as a tool for screening alternative feedstocks for production of biodiesel

    Science.gov (United States)

    Fatty acid (FA) composition was used as a screening tool for the selection of feedstocks high in monounsaturated content for evaluation as biodiesel. The feedstocks were ailanthus (Ailanthus altissima), anise (Pimpinella anisum), arugula (Eruca vesicaria), camelina (Camelina sativa), coriander (Cori...

  6. Fatty acid profile as a basis for screening feedstocks for biodiesel production

    Science.gov (United States)

    Fatty acid (FA) profile was used as a screening tool for the selection of feedstocks high in monounsaturated content for evaluation as biodiesel. The feedstocks were ailanthus (Ailanthus altissima), anise (Pimpinella anisum), arugula (Eruca vesicaria), camelina (Camelina sativa), coriander (Coriandr...

  7. Avaliação biológica de Foeniculum vulgare (Mill. (Umbelliferae/Apiaceae Biological evaluation of Foeniculum vulgare (Mill. (Umbelliferae/Apiaceae

    Directory of Open Access Journals (Sweden)

    O. Araujo R

    2013-01-01

    Full Text Available Foeniculum vulgare Mill. é espécie herbácea da família Umbelliferae, nativa da região do Mediterrâneo e da Ásia Menor, amplamente distribuída em todo o território brasileiro. É conhecida popularmente como funcho ou erva-doce e usada na medicina como analgésico, digestivo, carminativo, diurético, expectorante, lactígeno, anti-inflamatório, e antiespasmódico. O extrato bruto etanólico para a verificação das atividades biológicas foi preparado a partir de sementes compradas no comércio. Para a realização do perfil fitoquímico foi utilizada a cromatografia em camada delgada analítica; a atividade antimicrobiana foi determinada pelo teste de difusão em disco de papel e da concentração inibitória mínima (CIM; a atividade antinociceptiva foi realizada pelo método de contorções abdominais em cobaias. Os micro-organismos testados foram isolados clínicos multirresistentes obtidos do Setor de Bacteriologia do Hospital das Clínicas de Pernambuco. O estudo fitoquímico identificou a maioria dos compostos secundários presentes na fração metanólica das sementes, sendo eles: triterpenos, glicosídeos de flavanóides, terpenos menores (monoterpenóides, sesquiterpenóide e diterpenóides, e açúcares redutores. Os resultados obtidos mostraram que o extrato etanólico apresentou maior atividade frente à Micrococcus spp. (CIM=250µg/mL. Os resultados da avaliação da atividade antinociceptiva demonstraram que apenas a dosagem de 298 mg/Kg quando comparado com o padrão indometacina conseguiu uma redução significativa no número de contorções abdominais dos animais. Estudos posteriores deverão ser realizados para a identificação e isolamento de alguns compostos secundários, bem como a realização de outros protocolos de analgesia.Foeniculum vulgare Mill. (Umbelliferae is a herbaceous plant of the family Umbelliferae, native to the Mediterranean and Asia Minor region and widely distributed all over the Brazilian

  8. Aspectos anatômicos de plântulas Foeniculum vulgare Mill. Anatomical aspects of the Foeniculum vulgare Mill. seedling

    Directory of Open Access Journals (Sweden)

    C.F. Azevedo

    2012-01-01

    Full Text Available A erva-doce, Foeniculum vulgare Mill. (Apiaceae, é uma erva entouceirada, aromática que apresenta propriedades condimentares e medicinais; tem origem Européia e é amplamente cultivada em todo o Brasil. Devido à deficiência de informações relativas à organização estrutural de plântulas de espécies medicinais, o presente estudo teve como objetivo fornecer informações sobre a anatomia e o desenvolvimento de plântulas de F. vulgare. As sementes de erva-doce foram semeadas em areia e mantidas em casa de vegetação por 25 dias, sendo realizadas regas diárias. Foram selecionadas plântulas normais e de padrão uniforme, que tiveram raiz, zona de transição, caule, cotilédones e primeiras folhas seccionados à mão livre. Este material foi corado e montado em lâminas com glicerina para observação em microscópio. Em todos os órgãos da plântula de erva-doce a epiderme é unisseriada, cuticularizada e com estômatos; sendo que as duas últimas estruturas não são encontradas na raiz, porém esta apresenta pêlos unicelulares e cônicos. Os cotilédones e as folhas apresentam funções fotossintéticas e nutricionais, indicadas pela presença de grande quantidade de estômatos, cloroplastos e amido. A caracterização anatômica de plântulas de F. vulgare auxilia na identificação da espécie e no conhecimento da organização celular, fornecendo informações que auxiliam em estudos fisiológicos, taxonômicos e ecológicos.Fennel, Foeniculum vulgare Mill. (Apiaceae, is a forming clumps herb and presents aromatic, medicinal and condimental properties. It has European origin and is widely cultivated and used in Brazil. Due to lack of information concerning the structural organization of seedlings of medicinal species, this study aimed to provide information about the anatomy and development of F. vulgare seedlings. The seeds fennel was sowed in sand and maintained in greenhouse for 25 days, with daily waterings. Were selected

  9. Chemical compositions and antioxidant properties of essential oils from nine species of Turkish plants obtained by supercritical carbon dioxide extraction and steam distillation.

    Science.gov (United States)

    Topal, Ummihan; Sasaki, Mitsuru; Goto, Motonobu; Otles, Semih

    2008-01-01

    Chemical compositions and antioxidant activities of essential oils from nine different species of Turkish plants, namely Melissa officinalis L., Rosmarinus officinalis L., Cuminum cyminum L., Piper nigrum L., Lavandula stoechas spp., Foeniculum vulgare, Pimpinella anisum L., Thymus serpyllum and Liquidamber orientalis Mill., were studied. Essential oils were obtained by supercritical carbon dioxide (SCCO2) extraction and steam distillation, and were analyzed by gas chromatography-mass spectrometry. The antioxidant activities of SCCO2 extraction and steam distillation extracts were tested by means of the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Essential oils extracted by SCCO2 and steam distillation showed different compositions in different species. In the DPPH assay, R. officinalis, C. cyminum, P. anisum, T. serpyllum and L. orientalis essential oils obtained by SCCO2 extraction showed higher antioxidant activity than steam distillation extracts, with radical scavenging activities ranging from 87.1 +/- 0.23% to 92.0 +/- 0.34% compared with the butylated hydroxytoluene positive control (91.4 +/- 0.21%).

  10. Hyadaphis foeniculi na cultura de erva-doce no Estado de Pernambuco Hyadaphis foeniculi on anise crop in Pernambuco State

    Directory of Open Access Journals (Sweden)

    Rachel Gonçalves Ferreira

    2004-12-01

    Full Text Available Hyadaphis foeniculi (Passerini, 1860 (Hemiptera: Aphididae tem sido observado causando danos à cultura de erva-doce, Pimpinella anisum L., na região do agreste meridional de Pernambuco. Este é o primeiro registro da espécie no Estado.Hyadaphis foeniculi (Passerini, 1860 (Hemiptera: Aphididae was observed causing damage on anise crop, Pimpinella anisum L., in the Meridional "Agreste" region of Pernambuco State, Brazil. This is the first report of this species in the region.

  11. Antibacterial activity against Clostridium genus and antiradical activity of the essential oils from different origin.

    Science.gov (United States)

    Kačániová, Miroslava; Vukovič, Nenad; Horská, Elena; Salamon, Ivan; Bobková, Alica; Hleba, Lukáš; Fiskelová, Martina; Vatľák, Alexander; Petrová, Jana; Bobko, Marek

    2014-01-01

    In the present study, the antimicrobial and antiradical activities of 15 essential oils were investigated. The antimicrobial activities were determined by using agar disc diffusion and broth microdilution methods against Clostridium genus and antioxidant properties of essential oils by testing their scavenging effect on DPPH radicals activities. We determined the antibacterial activity of Clostridium butyricum, Clostridium hystoliticum, Clostridium intestinale, Clostridium perfringens and Clostridium ramosum. We obtained the original commercial essential oils samples of Lavandula angustifolia, Carum carvi, Pinus montana, Mentha piperita, Foeniculum vulgare Mill., Pinus sylvestris, Satureia montana, Origanum vulgare L. (2 samples), Pimpinella anisum, Rosmarinus officinalis L., Salvia officinalis L., Abies alba Mill., Chamomilla recutita L. Rausch and Thymus vulgaris L. produced in Slovakia (Calendula a.s., Nova Lubovna, Slovakia). The results of the disk diffusion method showed very high essential oils activity against all tested strains of microorganisms. The best antimicrobial activity against C. butyricum was found at Pimpinella anisum, against C. hystoliticum was found at Pinus sylvestris, against C. intestinale was found at Satureia hortensis L., against C. perfringens was found at Origanum vulgare L. and against C. ramosum was found at Pinus sylvestris. The results of broth microdilution assay showed that none of the essential oils was active against C. hystoliticum. The best antimicrobial activity against C. butyricum was found at Abies alba Mill., against C. intestinale was found at Abies alba Mill., against C. perfringens was found at Satureia montana and against C. ramosum was found at Abius alba and Carum carvi. Antioxidant DPPH radical scavenging activity was determined at several solutions of oil samples (50 μL.mL(-1)-0.39 μL.mL(-1)) and the best scavenging effect for the highest concentration (50 μL.mL(-1)) was observed. The antioxidant properties

  12. 小茴香生理活性成分的研究进展%Research progress in physiologically active compounds of Foeniculum vulgare Mill

    Institute of Scientific and Technical Information of China (English)

    赵秀玲

    2013-01-01

    对小茴香的生理活性成分及其提取分离、保健作用等研究作了归纳与论述,为小茴香在食品和医药上的进一步研究开发提供理论依据.%Extraction,seperation and health function of Foeniculum vulgare Mill were summarized and discussed respectively. It provided theoretical evidence for future research and development of Foeniculum vulgare Mill in food industry and medicine.

  13. Foeniculum vulgare: A comprehensive review of its traditional use, phytochemistry, pharmacology, and safety

    Directory of Open Access Journals (Sweden)

    Manzoor A. Rather

    2016-11-01

    Full Text Available Foeniculum vulgare (Apiaceae commonly known as fennel is a well known and important medicinal and aromatic plant widely used as carminative, digestive, lactogogue and diuretic and in treating respiratory and gastrointestinal disorders. Its seeds are used as flavourings in baked goods, meat and fish dishes, ice cream, alcoholic beverages and herb mixtures. Phenols, phenolic glycosides and volatile aroma compounds such as trans-anethole, estragole and fenchone have been reported as the major phytoconstituents of this species. Different pharmacological experiments in a number of in vitro and in vivo models have convincingly demonstrated the ability of F. vulgare to exhibit antifungal, antibacterial, antioxidant, antithrombotic and hepatoprotective activities, lending support to the rationale behind several of its therapeutic uses. Phenolic compounds isolated from F. vulgare are considered to be responsible for its antioxidant activity while the volatile aroma compounds make it an excellent flavouring agent. The present review is an up-to-date and comprehensive analysis of the chemistry, pharmacology, traditional uses and safety of F. vulgare.

  14. Beneficial effects of Foeniculum vulgare on ethanol-induced acute gastric mucosal injury in rats

    Institute of Scientific and Technical Information of China (English)

    Fatih Mehmet Birdane; Mustafa Cemek; Yavuz Osman Birdane; (I)lhami Gül(c)in; Mehmet Emin Büyükokuro(g)lu

    2007-01-01

    AIM: To examine the anti-ulcerogenic and antioxidant effects of aqueous extracts of Foeniculum vulgare (FVE) on ethanol-induced gastric lesions in rats.METHODS: FVE was administered by gavage at doses of 75, 150 and 300 mg/kg, and famotidine was used at the dose of 20 mg/kg. Following a 60 min period, all the rats were given 1 mL of ethanol (80%) by gavage. One hour after the administration of ethanol, all groups were sacrificed, and the gastric ulcer index was calculated;whole blood malondialdehyde (MDA) and reduced glutathione (GSH), serum nitrate, nitrite, ascorbic acid,retinol and β-carotene levels were measured in all the groups.RESULTS: It was found that pretreatment with FVE significantly reduced ethanol-induced gastric damage.This effect of FVE was highest and statistically significant in 300 mg/kg group compared with the control (4.18 ± 2.81 vs 13.15 ± 4.08, P < 0.001). Also, pretreatment with FVE significantly reduced the MDA levels, while significantly increased GSH, nitrite, nitrate, ascorbic acid,retinol and β-carotene levels.CONCLUSION: FVE has clearly a protective effect against ethanol-induced gastric mucosal lesion, and this effect, at least in part, depends upon the reduction in lipid peroxidation and augmentation in the antioxidant activity.

  15. Study of Foeniculum vulgare (Fennel Seed Extract Effects on Serum Level of Oxidative Stress

    Directory of Open Access Journals (Sweden)

    Sadeghpour Nahid

    2015-04-01

    Full Text Available Objective: The Foeniculum vulgare (FVE, known as fennel, has a long history of herbal uses as both food and medicine. The seed of this plant has been used to promote menstruation, alleviate the symptoms of female climacteric, and increase the number of ovarian follicles. The aim of this study was to evaluate the fennel extract effects on serum level of oxidative stress in female mice. Materials and Methods: Totally, 28 virgin female albino mice were divided into four groups (n = 7. Groups 1 and 2 (experimental groups were administered FVE at 100 and at a concentration of 100 and 200 mg/kg for 5 days, interaperitoneally. Group 3 (negative control received ethanol and Group 4 (positive control received normal saline. Animals were scarified at 6th day, sera were collected and the level of oxidative stress was determination of using total antioxidant status kit. Results: Data analysis revealed that there is a significant difference in the mean level of serum oxidative stress between four different groups. P value in experimental groups compared to the control group was (P < 0.0001. Conclusion: Fennel extract can decrease the serum level of oxidative factors in female mice; it can be introduced as a novel medicine for treatment of infertility

  16. 5-Lipoxygenase Inhibition of the Fructus of Foeniculum vulgare and Its Constituents.

    Science.gov (United States)

    Lee, Je Hyeong; Lee, Dong Ung; Kim, Yeong Shik; Kim, Hyun Pyo

    2012-01-01

    The fruits of Foeniculum vulgare (Foeniculi Fructus) have been widely used in Chinese medicine as an antiemetic, ameliorating stomach ailments and as an analgesic. In order to establish its potential for antiallergic use, inhibitory actions of the fruiton 5-lipoxgenase (5-LOX) and β-hexosaminidase release were evaluated. The 70% ethanol extract of this plant material (FR) considerably inhibited 5-LOX-catalyzed leukotriene production from A23187-induced rat basophilic leukemia (RBL)-1 cells. The IC50 was 3.2 μg/ml. From this extract, 12 major compounds including sabinene, fenchone, γ-terpinene, α-pinene, limonene, p-anisylacetone, p-anisylaldehyde, estragole (4-allylanisole), trans-anethole, scopoletin, bergapten and umbelliferone were isolated. And it was found that several terpene derivatives including γ-terpinene and fenchone as well as phenylpropanoid, trans-anethole, showed considerable inhibitory action of 5-LOX. In particular, the IC50 of trans-anethole was 51.6 μ M. In contrast, FR and the isolated compounds did not show considerable inhibitory activity on the degranulation reaction of β-hexosaminidase release from antigen-treated RBL-2H3 cells. Against arachidonic acid-induced ear edema in mice, FR and trans-anethole showed significant inhibition by oral administration at doses of 100-400 mg/kg. In conclusion, FR and several major constituents are 5-LOX inhibitors and they may have potential for treating 5-LOX-related disorders.

  17. Susceptibility of Two Sitophilus species (Coleoptera: Curculionidae to Essential Oils from Foeniculum vulgare and Satureja hortensis

    Directory of Open Access Journals (Sweden)

    Asgar Ebadollahi

    2011-12-01

    Full Text Available This study was conducted to determine the insecticidal activity of essential oils from Fennel, Foeniculum vulgare (Apiaceae, and Summer savory, Satureja hortensis (Lamiaceae, against two stored-product insects. Essential oils from two species of plants were obtained by Clevenger-type water distillation and their fumigant toxicities were tested against adults of the wheat weevil, Sitophilus granarius and rice weevil, Sitophilus oryzae (Curculionidae. The mortality was determined after 24 and 48 hrs from beginning of exposure. LC50 values of each essential oil were estimated for each insect species. Fumigation bioassays revealed that essential oils from two plants had strong insecticidal activity on experimental insects. LC50 values indicated that S. granarius was more susceptible than S. oryzae to essential oils at the exposure time 24 and 48 hrs. The mortality effect of S. hortensis oil was lower than F. vulgare oil. The LC50 values decreased with the duration of exposure to the essential oil concentrations. In all case, responses varied according to plant material, concentration, and exposure time. These results indicated that essential oils from S. hortensis and F. vulgare could be applicable to the management of stored product insects to decrease ecologically detrimental effects of utilization synthetic insecticides.

  18. Cytotoxicity of syringin and 4-methoxycinnamyl alcohol isolated from Foeniculum vulgare on selected human cell lines.

    Science.gov (United States)

    Lall, Namrita; Kishore, Navneet; Binneman, Brigitte; Twilley, Danielle; van de Venter, Maryna; du Plessis-Stoman, Debbie; Boukes, Gerhardt; Hussein, Ahmed

    2015-01-01

    This study was carried out to determine the cytotoxic effect of seven plant extracts and the isolated compounds - syringin and 4-methoxycinnamyl alcohol - on cancerous and non-cancerous cells. The ethanol extract of Foeniculum vulgare was found to exhibit the most significant toxicity with an IC50 value of 19.97 μg/mL on HeLa cells. Bioassay-guided fractionation led to the isolation of two compounds, syringin (1) and 4-methoxycinnamyl alcohol (2). Both compounds showed toxicity against MCF-7, HeLa and DU145 cancer cell line. The results showed that compound 2 showed high toxicity against all the cancer cell lines with IC50 values of 14.24, 7.82 and 22.10 μg/mL, respectively. 4-Methoxycinnamyl alcohol also showed no apoptotic effect in cell cycle analysis after 48 h at a concentration of 10 μg/mL. However, DNA fragmentation study revealed that necrosis took place at a concentration of 10 μg/mL after 48 h exposure.

  19. Anthelmintic effects of the essential oil of fennel (Foeniculum vulgare Mill., Apiaceae) against Schistosoma mansoni.

    Science.gov (United States)

    Wakabayashi, Kamila A L; de Melo, Nathalya I; Aguiar, Daniela P; de Oliveira, Pollyanna F; Groppo, Milton; da Silva Filho, Ademar A; Rodrigues, Vanderlei; Cunha, Wilson R; Tavares, Denise C; Magalhães, Lizandra G; Crotti, Antônio E M

    2015-07-01

    Foeniculum vulgare Mill. (Apiaceae), known as fennel, is a widespread aromatic herbaceous plant, and its essential oil is used as additive in the food, pharmaceutical, cosmetic, and perfume industries. The in vitro antischistosomal activity and cytotoxic effects against V79 cells of the essential oil of F. vulgare cultivated in southeastern Brazil (FV-EO) was investigated. The FV-EO was obtained by hydrodistillation and characterized by GC-FID and GC/MS analyses. (E)-Anethole (69.8%) and limonene (22.5%) were identified as the major constituents. Its anthelmintic activity against Schistosoma mansoni was evaluated at concentrations of 10, 50, and 100 μg/ml, and it was found to be active against adult S. mansoni worms, although it was less effective than the positive control praziquantel (PZQ) in terms of separation of the coupled pairs, mortality, and decreased motor activity. However, FV-EO elicited an interesting dose-dependent reduction in the number of S. mansoni eggs. On their own, (E)-anethole and the limonene enantiomers were much less effective than FV-EO and PZQ. An XTT-cytotoxicity-based assay evidenced no FV-EO cytotoxicity against V79 cells. In summary, FV-EO displayed moderate in vitro schistosomicidal activity against adult S. mansoni worms, exerted remarkable inhibitory effects on the egg development, and was of low toxicity.

  20. Antifungal properties of Foeniculum vulgare, Carum carvi and Eucalyptus sp. essential oils against Candida albicans strains

    Directory of Open Access Journals (Sweden)

    Skrobonja Jelica M.

    2013-01-01

    Full Text Available Aromatic plants are among the most important sources of biologically active secondary metabolites, with high antimicrobal potential. This study was carried out to examine in vitro antifungal activity of Foeniculum vulgare (Apiaceae, Carum carvi (Apiaceae and Eucalyptus sp.(Myrtaceae essential oils against three Candida albicans strains of different origin (laboratory-CAL, human pulmonary-CAH and ATCC10231-CAR. The essential oils were screened on C. albicans using disc and well-diffusion and microdilution method, and compared to Nystatine and Fluconazole as standard anti-mycotics. The activity of tested oils was expressed by inhibition zone diameter (mm, minimum inhibitory concentration (MIC and minimum fungicidal concentration (MFC (mg/ml. The results indicated that studied essential oils show antifungal activity against all three isolates of C. albicans. It was observed that each oil exhibits different degree of antifungal activity depending on the oil concentration applied as well as on analyzed strain of C. albicans. Carum carvi demonstrated the strongest antifungal effect to all tested strains, showing the lowest MIC values (0.03mg/ml for CAL, 0.06mg/ml for CAH, and 0.11mg/ml for CAR, respectively. Eucalyptus sp. exhibited the lowest antifungal activity, with MIC values ranging from 0.11 mg/ml for CAL to 0.45 mg/ml for both CAH and CAR. [Projekat Ministarstva nauke Republike Srbije, br. 172058

  1. [Determination of thirteen metal elements in the plant Foeniculum vulgare Mill. by flame atomic absorption spectrophotometry].

    Science.gov (United States)

    Xue, Guo-qing; Liu, Qing; Han, Yu-qi; Wei, Hui-guang; Dong, Ting

    2006-10-01

    The objective of the paper is to determine the amount of metal elements of Na, K, Mg, Ca, Cu, Zn, Mn, Fe, Co, Ni, Cd, Cr and Pb in the planted Foeniculum vulgare Mill. by flame atomic absorption spectrophotometry (FAAS), after the cinefaction and the digestion with HNO3-HClO4 (phi 4:1) at 90-95 degrees C and normal pressure. The optimum parameters of FAAS and the effects of solution medium on the results were investigated. The analytical results show that the amount of Na, K, Mg, Ca, Mn, Fe, Cu, Zn and Pb was 1508.7, 27653.0, 2036.0, 4848.1, 24.8, 323.5, 15.2, 23.7 and 10.8 microg x g(-1), respectively, and that of Co, Ni, Cd and Cr was not checked out in the samples. The recovery of standard addition is 97.45%-102.50%, the relative standard deviation (n=9) was 0.34%-2.77%. The characteristic method is quick, simple and convenient and the results are satisfactory.

  2. Assessment of safe bioactive doses of Foeniculum vulgare Mill. essential oil from Portugal.

    Science.gov (United States)

    Cabral, C; Miranda, M; Gonçalves, M J; Cavaleiro, C; Cruz, M T; Salgueiro, L

    2017-03-02

    This study was designed to evaluate the antifungal activity of Foeniculum vulgare essential oil; concomitantly, the safety of bioactive doses was also unveiled, for the first time, in several mammalian cells. The chemical characterisation was made by GC and GC-MS. Antifungal activity was evaluated against yeasts, dermatophytes and Aspergillus strains and germ tube inhibition assay was evaluated using Candida albicans. Cell viability was assessed by the MTT assay. The main constituents of the oil are E-anetol (47%), α-phellandrene (11%), α-pinene (10.1%) and fenchone (10.8%). The oil was more active against Cryptococcus neoformans and C. albicans (MICs 0.32-0.64 μL/mL) and the filamentation of C. albicans was totally inhibited with 0.08 μL/mL. The oil is safe for keratinocytes, hepatocytes and fibroblasts in concentrations up to 1.25 μL/mL, and to macrophages up to 0.64 μL/mL. These findings highlight safe bioactive concentrations that should be deeper investigated for further application in pharmaceutical industry.

  3. Major essential oils composition and immunotoxicity activity from leaves of Foeniculum vulgare against Aedes aegypti L.

    Science.gov (United States)

    Chung, Ill-Min; Ro, Hee-Myong; Moon, Huyng-In

    2011-09-01

    The leaves of Foeniculum vulgare (Umbelliferae) were extracted and the major essential oil composition and immunotoxicity effects were studied. The analyses conducted by gas chromatography and mass spectroscopy (GC-MS) revealed the essential oils of F. vulgare leaves. The F. vulgare essential oil yield was 0.97%, and GC/MS analysis revealed that its major constituents were methyl clavicol (46.3%), α-phellandrene (18.2%), fenchone (10.6%), (E)-anethole (11.3%), myrcene (3.4%), and α-pinene (2.1%). The essential oil had a significant toxic effect against early fourth-stage larvae of Aedes aegypti L with an LC(50) value of 41.23 ppm and an LC(90) value of 65.24 ppm. Also, methyl clavicol (≥98.0%), α-phellandrene (≥95.0%), fenchone (≥98.0%), (E)-anethole (≥99.0%), myrcene (≥99.0%), and α-pinene (≥99.0%) were tested against the F(21) laboratory strain of A. aegypti. Fenchone (≥98.0%) and (E)-anethole (≥99.0%) have medium activity with an LC(50) value of 73.11 ppm and 102.41 ppm. The above data indicate that major compounds interaction may play a more important role in the toxicity of essential oil.

  4. Essential oil composition of Foeniculum vulgare Mill. fruits from pharmacies in different countries.

    Science.gov (United States)

    Raal, Ain; Orav, Anne; Arak, Elmar

    2012-01-01

    Variations in the essential oil composition of Foeniculum vulgare Mill. commercial fruits obtained from retail pharmacies in Estonia, Norway, Austria and Moldova and from a spice shop in Turkey were determined using capillary GC techniques. The essential oil content of all the samples was 5-51 mL kg(-1) and between 22 and 51 mL kg(-1) in fennel fruits bought from pharmacies. A total of 34 compounds were identified. The major component was trans-anethole (34.8-82.0%); the other principal compounds in oils were fenchone (1.6-22.8%), estragole (2.4-17.0%), limonene (0.8-16.5%), and cis-anethole (0.1-8.6%). The yield of essential oil (5.0 mL kg(-1)) and content of trans-anethole was very low (34.8%) in the Turkish spice sample. Maximum yield of essential oil was found in fennel from Norway and Austria (50.7 and 50.5 mL kg(-1), respectively); these samples were rich in fenchone (21.2% and 22.8%), but contained less trans-anethole (64.6-63.7) than samples from Estonia and Moldova (82.0% and 80.9%). The typical samples of sweet fennel (bought from Estonia and Moldova) and bitter fennel (from Norway and Austria) were found to conform completely or partially to EP standards, although fennel type was always not marked on the packages.

  5. Distinguishing Foeniculum vulgare fruit from two adulterants by combination of microscopy and GC-MS analysis.

    Science.gov (United States)

    Ma, Xiao-Dong; Mao, Wen-Wen; Zhou, Ping; Li, Ping; Li, Hui-Jun

    2015-07-01

    Foeniculum vulgare fruit (FVF) is a widely used traditional medicine. However, two adulterants, namely Anethum graveolens fruit (AGF) and Cuminum cyminum fruit (CCF), have been found in use as FVF in China owing to similar appearance and odor. For the purpose of accurate differentiation of the three herbal medicines, extensive anatomical examination and chemical profiling were conducted. Using light microscopy and fluorescence microscopy, the macroscopic and microscopic features of the three species were compared. It was found that some microscopic characteristics, including transverse shape of mericarp, presence or absence of reticulate cells and non-glandular hairs, as well as fluorescence of endocarp, were of diagnostic significance. Moreover, essential oils were qualitatively and semi-quantitatively analyzed using GC-MS. The analytical results indicated significant chemical variations in different species: in FVF, trans-anethole (83.20%) was the predominant volatile compound followed by estragole (5.03%) and limonene (3.45%), while in AGF, the first, second and third compounds with highest content were carvone (42.58%), apiol (20.76%) and limonene (20.32%), and in CCF were cuminlaldehyde (36.00%), 2-caren-10-al (23.25%) and γ-terpinene (9.65%), respectively. In conclusion, the proposed light microscopy coupled with fluorescence microscopy and/or GC-MS analysis allowed successful distinguishing FVF from AGF and CCF.

  6. Antimycobacterial Activity of Constituents from Foeniculum vulgare Var. Dulce Grown in Mexico

    Directory of Open Access Journals (Sweden)

    María del Rayo Camacho-Corona

    2012-07-01

    Full Text Available Bioassay guided fractionation of an antimycobacterial extract of Foeniculum vulgare var dulce (Apiaceae led to the isolation and characterization of 5-hydroxyfurano-coumarin. The chemical structure of this compound was elucidated by 1H and 13C (1D and 2D Nuclear Magnetic Resonance (NMR spectroscopy. In addition, the active fractions were analyzed by GC-MS and seventy eight compounds were identified; the major compounds were 1,3-benzenediol, 1-methoxycyclohexene, o-cymene, sorbic acid, 2-hydroxy-3-methyl-2-cyclopenten-1-one, estragole, limonene-10-ol and 3-methyl-2-cyclopenten-1-one. Twenty compounds identified in the active fractions were tested against one sensitive and three MDR strains of Mycobacterium tuberculosis using the Alamar Blue microassay. Compounds that showed some degree of antimycobacterial activity against all strains tested were the following: linoleic acid (MIC 100 µg/mL, oleic acid (MIC 100 µg/mL, 1,3-benzenediol (MIC 100–200 µg/mL, undecanal (MIC 50–200 µg/mL, and 2,4-undecadienal (MIC 25–50 µg/mL, the last being the most active compound. To our knowledge, this is the first report of the presence of 5-hydroxy-furanocoumarin in F. vulgare.

  7. Protective effect of Foeniculum vulgare essential oil and anethole in an experimental model of thrombosis.

    Science.gov (United States)

    Tognolini, Massimiliano; Ballabeni, Vigilio; Bertoni, Simona; Bruni, Renato; Impicciatore, Mariannina; Barocelli, Elisabetta

    2007-09-01

    In a previous screening work, Foeniculum vulgare essential oil emerged from a pool of 24 essential oils for its antiplatelet properties and its ability to destabilize the retraction of the coagulum. In the present work the main component of the oil, anethole, tested in guinea pig plasma was as potent as fennel oil in inhibiting arachidonic acid-, collagen-, ADP- and U46619-induced aggregation (IC(50) from 4 to 147 microg ml(-1)). It also prevented thrombin-induced clot retraction at concentrations similar to fennel oil. The essential oil and anethole, tested in rat aorta with or without endothelium, displayed comparable NO-independent vasorelaxant activity at antiplatelet concentrations which have been proved to be free from cytotoxic effects in vitro. In vivo, both F. vulgare essential oil and anethole orally administered in a subacute treatment to mice (30 mg kg(-1)day(-1) for 5 days) showed significant antithrombotic activity preventing the paralysis induced by collagen-epinephrine intravenous injection (70% and 83% protection, respectively). At the antithrombotic dosage they were free from prohemorrhagic side effect at variance with acetylsalicylic acid used as reference drug. Furthermore, both F. vulgare essential oil and anethole (100 mg kg(-1) oral administration) provided significant protection toward ethanol induced gastric lesions in rats. In conclusion, these results demonstrate for F. vulgare essential oil, and its main component anethole, a safe antithrombotic activity that seems due to their broad spectrum antiplatelet activity, clot destabilizing effect and vasorelaxant action.

  8. Antimycobacterial activity of constituents from Foeniculum vulgare var. dulce grown in Mexico.

    Science.gov (United States)

    Esquivel-Ferriño, Patricia C; Favela-Hernández, Juan Manuel J; Garza-González, Elvira; Waksman, Noemí; Ríos, María Yolanda; del Rayo Camacho-Corona, María

    2012-07-13

    Bioassay guided fractionation of an antimycobacterial extract of Foeniculum vulgare var dulce (Apiaceae) led to the isolation and characterization of 5-hydroxyfurano-coumarin. The chemical structure of this compound was elucidated by 1H and 13C (1D and 2D) Nuclear Magnetic Resonance (NMR) spectroscopy. In addition, the active fractions were analyzed by GC-MS and seventy eight compounds were identified; the major compounds were 1,3-benzenediol, 1-methoxycyclohexene, o-cymene, sorbic acid, 2-hydroxy-3-methyl-2-cyclopenten-1-one, estragole, limonene-10-ol and 3-methyl-2-cyclopenten-1-one. Twenty compounds identified in the active fractions were tested against one sensitive and three MDR strains of Mycobacterium tuberculosis using the Alamar Blue microassay. Compounds that showed some degree of antimycobacterial activity against all strains tested were the following: linoleic acid (MIC 100 µg/mL), oleic acid (MIC 100 µg/mL), 1,3-benzenediol (MIC 100-200 µg/mL), undecanal (MIC 50-200 µg/mL), and 2,4-undecadienal (MIC 25-50 µg/mL), the last being the most active compound. To our knowledge, this is the first report of the presence of 5-hydroxy-furanocoumarin in F. vulgare.

  9. Medicinal properties of Foeniculum vulgare Mill. in traditional Iranian medicine and modern phytotherapy.

    Science.gov (United States)

    Rahimi, Roja; Ardekani, Mohammad Reza Shams

    2013-01-01

    Foeniculum vulgare Mill. (F. vulgare), commonly known as Fennel, is a popular medicinal plant with various pharmacological activities mentioned in traditional Iranian medicine (TIM) and modern phytotherapy such as antioxidant, cytotoxic, anti-inflammatory, antimicrobial, bronchodilatory, estrogenic, diuretic, lithontripic, galactogogue, emmenagogue, antithrombotic, hypotensive, gastroprotective, hepatoprotective, memory enhancing, and antimutagenic activities. No serious adverse events were recorded after ingestion of F. vulgare except some cases of allergic reactions. The estrogenic activity of F. vulgare brings some side effects such as decrease in protein concentration and acid and alkaline phosphatase in male genital organs, increase in weight of mammary glands and reproductive organs in women and premature thelarche in girls. However, no evidence of teratogenicity was recorded, it is better not to use F. vulgare during pregnancy due to its estrogenic activity. Because of inhibition of cytochrome P450 3A4 (CYP3A4), the pharmacokinetic parameters of drugs mainly metabolized by this isozyme may be affected by F. vulgare. In addition, a significant interaction between cyprofloxacin and F. vulgare was demonstrated. The aim of current paper is to review pharmacological properties, toxicity and adverse events, and drug interactions of vulgare and brings conclusive results about the use of this plant in men, women and during pregnancy.

  10. 山黄皮上柑橘木虱发生调查初报%Preliminary reports on host-vector relationship between Clausena anisum-olens and Citrus psylla

    Institute of Scientific and Technical Information of China (English)

    陈国平; 刘冰浩; 冷付春; 牛英; 王小欣; 李子健; 陈传武; 陈海生; 梁其波

    2011-01-01

    [目的]明确山黄皮上是否有柑橘木虱为害,为开展山黄皮是否是柑橘木虱寄主研究奠定基础.[方法]采用面上调查和实地调查相结合的方法,对地处广西桂南的龙州县、宁明县及广西现代农业技术展示中心(南宁)的山黄皮、黄皮、柑橘上柑橘木虱发生情况进行调查.[结果]被调查的12个点中,广西现代农业技术展示中心的山黄皮上发现有柑橘木虱,其他调查点山黄皮上未发现有柑橘木虱.[结论]山黄皮上有柑橘木虱,但山黄皮是否是柑橘木虱的寄主尚待研究.%[Objective]The present experiment was conducted to determine the damages of Clausena anisum-olens caused by Citrus psylla in order to find out host-vector relationship amongst Clausena anisum-olens and Citrus psylla. [ Method ]The field survey was undertaken to investigate the current status of Citrus psylla occurrence on Clausena anisum-olens, Clausena lansium, Citrus limon and citrus trees of Longzhou, Ningming in Guangxi and the Guangxi Exhibi-tion Centre of Modern Agriculture Technology (Nanning). [Result]Except the Clausena anisum-olens trees in the Exhibi-tion Centre of Guangxi Modern Agriculture Technology, no other incidences of Citrus psyalla occurrence on Clausena anisum-olens were observed at 11 investigation sites. [ Conclusion ]The presence of Citrus psylla on Clausena anisum-olens trees was established, but relationship with Clausena anisum-olens as host plant for Citrus psylla still needs further in-vestigations.

  11. Improved growth and essential oil yield and quality in Foeniculum vulgare mill on mycorrhizal inoculation supplemented with P-fertilizer.

    Science.gov (United States)

    Kapoor, Rupam; Giri, Bhoopander; Mukerji, Krishna G

    2004-07-01

    Two arbuscular mycorrhizal (AM) fungi Glomus macrocarpum and Glomus fasciculatum significantly improved growth and essential oil concentration of Foeniculum vulgare Mill. However, AM inoculation of plants along with phosphorus fertilization significantly enhanced growth, P-uptake and essential oil content of plants compared to either of the components applied separately. Among the two fungal inoculants, G. fasciculatum registered the highest growth at both levels of phosphorus used with up to 78% increase in essential oil concentration of fennel seeds over non-mycorrhizal control. The essential oil characterization by gas liquid chromatography revealed that the level of anethol was significantly enhanced on mycorrhization.

  12. Comparing the effects of aerobic exercise and Foeniculum vulgare on pre-menstrual syndrome

    Directory of Open Access Journals (Sweden)

    Hassan Pazoki

    2016-03-01

    Full Text Available Introduction: Premenstrual syndrome (PMS has been identified by a number of psychological and physical symptoms which occur cyclically in the luteal phase of the menstrual cycle. The present study has been carried out to compare the effects of regular exercise and Foeniculum vulgare extract (fennel together and separately on PMS in high school girls. Materials and methods: In this randomized clinical trial 48 students aged 16–18 years were selected by filling the daily record of severity of problem questionnaire (DRSP-Q. The participants were divided into four equal groups: the first group received fennel, the second group had aerobic exercise, the third group received fennel along with exercise and the last group was control group without fennel and exercise. Participants filled DRSP-Q three times: the first menstrual cycle before the intervention, the first menstrual cycle after four weeks and finally the first menstrual cycle after eight weeks of intervention. Results: After 8 weeks of intervention the severity of PMS symptoms reduced significantly in experimental groups (fennel, exercise and fennel + exercise compared to control group (P < 0.05. Meanwhile, there were not any significant differences in age, body mass index, age at menarche, age at dysmenorrhea onset and duration of menstruation among the four groups. Discussions: The result of this study indicated that fennels and exercise could reduce the severity of premenstrual syndrome. In addition, fennel extract and exercise together seem to be more effective on symptoms of anxiety and depression compared with using them alone.

  13. Antibacterial and phytochemical screening of Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi

    Science.gov (United States)

    Kaur, Gurinder J; Arora, Daljit S

    2009-01-01

    Background Anethum graveolens Linn., Foeniculum vulgare Mill. and Trachyspermum ammi L. are widely used traditional medicinal plants to treat various ailments. To provide a scientific basis to traditional uses of these plants, their aqueous and organic seed extracts, as well as isolated phytoconstituents were evaluated for their antibacterial potential. Methods Antibacterial activity of aqueous and organic seed extracts was assessed using agar diffusion assay, minimum inhibitory concentration and viable cell count studies; and their antibacterial effect was compared with some standard antibiotics. The presence of major phytoconstituents was detected qualitatively and quantitatively. The isolated phytoconstituents were subjected to disc diffusion assay to ascertain their antibacterial effect. Results Hot water and acetone seed extracts showed considerably good antibacterial activity against all the bacteria except Klebsiella pneumoniae and one strain of Pseudomonas aeruginosa. Minimum inhibitory concentration for aqueous and acetone seed extracts ranged from 20–80 mg/ml and 5–15 mg/ml respectively. Viable cell count studies revealed the bactericidal nature of the seed extracts. Statistical analysis proved the better/equal efficacy of some of these seed extracts as compared to standard antibiotics. Phytochemical analysis showed the presence of 2.80 – 4.23% alkaloids, 8.58 – 15.06% flavonoids, 19.71 – 27.77% tannins, 0.55–0.70% saponins and cardiac glycosides. Conclusion Antibacterial efficacy shown by these plants provides a scientific basis and thus, validates their traditional uses as homemade remedies. Isolation and purification of different phytochemicals may further yield significant antibacterial agents. PMID:19656417

  14. Antibacterial and phytochemical screening of Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi

    Directory of Open Access Journals (Sweden)

    Arora Daljit S

    2009-08-01

    Full Text Available Abstract Background Anethum graveolens Linn., Foeniculum vulgare Mill. and Trachyspermum ammi L. are widely used traditional medicinal plants to treat various ailments. To provide a scientific basis to traditional uses of these plants, their aqueous and organic seed extracts, as well as isolated phytoconstituents were evaluated for their antibacterial potential. Methods Antibacterial activity of aqueous and organic seed extracts was assessed using agar diffusion assay, minimum inhibitory concentration and viable cell count studies; and their antibacterial effect was compared with some standard antibiotics. The presence of major phytoconstituents was detected qualitatively and quantitatively. The isolated phytoconstituents were subjected to disc diffusion assay to ascertain their antibacterial effect. Results Hot water and acetone seed extracts showed considerably good antibacterial activity against all the bacteria except Klebsiella pneumoniae and one strain of Pseudomonas aeruginosa. Minimum inhibitory concentration for aqueous and acetone seed extracts ranged from 20–80 mg/ml and 5–15 mg/ml respectively. Viable cell count studies revealed the bactericidal nature of the seed extracts. Statistical analysis proved the better/equal efficacy of some of these seed extracts as compared to standard antibiotics. Phytochemical analysis showed the presence of 2.80 – 4.23% alkaloids, 8.58 – 15.06% flavonoids, 19.71 – 27.77% tannins, 0.55–0.70% saponins and cardiac glycosides. Conclusion Antibacterial efficacy shown by these plants provides a scientific basis and thus, validates their traditional uses as homemade remedies. Isolation and purification of different phytochemicals may further yield significant antibacterial agents.

  15. Study of Foeniculum vulgare Effect on Folliculogenesis in Female Mice Kermanshah, Iran

    Directory of Open Access Journals (Sweden)

    Mohammad Rasool Khazaei

    2011-01-01

    Full Text Available Background: Foeniculum vulgare (FVE is used in traditional medicine for its antiseptic, palliativeand anti-inflammatory effects. Traditionally, FVE is utilized for treating female infertility. The presentstudy aims to investigate the effects of FVE extract on folliculogenesis in female albino mice.Materials and Methods: In this experimental study, a total of 20 female albino mice were divided into fourgroups. Groups 1 and 2 (experimental received FVE alcoholic extract at doses of 100 and 200 mg/kg bodyweight (BW/day for five days. Group 3 (negative control received ethanol and group 4 (positive controlwas administered normal saline, in the same doses as the experimental groups. Animals in all groups weresacrificed on the sixth day of the study; their ovaries were dissected out and prepared for histologicalexaminations. Hematoxylin and eosin (H&E stained microscopic slides were evaluated and the numbersof ovarian follicles were compared between groups. Data were analyzed by one way ANOVA.Results: The total follicle numbers were 26.5 ± 5.24 for group 1 (100 mg/kg FVE, 27.2 ± 4.1for group 2 (200 mg/kg FVE, 10.1 ± 2.53 for group 3 (ethanol control and 17.2 ± 3.9 for thesaline control group (group 4. The numbers of graffian, antral and multilaminar follicles increasedsignificantly in both experimental groups when compared with the control groups (p<0.05,however there were no significant differences in follicle numbers among the experimental groups.The number of unilaminar primary follicles did not significantly change between all groups. GCMSanalysis of FVE extract identified the presence of diosgenin, an estrogenic compound.Conclusion: FVE induced folliculogenesis in female mice ovary and increased the number ofgrowing ovarian follicles. The estrogenic component of FVE, diosgenin, may exert this effect.

  16. Native plant recovery in study plots after fennel (Foeniculum vulgare) control on Santa Cruz Island

    Science.gov (United States)

    Power, Paula; Stanley, Thomas R.; Cowan, Clark; Robertson, James R.

    2014-01-01

    Santa Cruz Island is the largest of the California Channel Islands and supports a diverse and unique flora which includes 9 federally listed species. Sheep, cattle, and pigs, introduced to the island in the mid-1800s, disturbed the soil, browsed native vegetation, and facilitated the spread of exotic invasive plants. Recent removal of introduced herbivores on the island led to the release of invasive fennel (Foeniculum vulgare), which expanded to become the dominant vegetation in some areas and has impeded the recovery of some native plant communities. In 2007, Channel Islands National Park initiated a program to control fennel using triclopyr on the eastern 10% of the island. We established replicate paired plots (seeded and nonseeded) at Scorpion Anchorage and Smugglers Cove, where notably dense fennel infestations (>10% cover) occurred, to evaluate the effectiveness of native seed augmentation following fennel removal. Five years after fennel removal, vegetative cover increased as litter and bare ground cover decreased significantly (P < 0.0001) on both plot types. Vegetation cover of both native and other (nonfennel) exotic species increased at Scorpion Anchorage in both seeded and nonseeded plots. At Smugglers Cove, exotic cover decreased significantly (P = 0.0001) as native cover comprised of Eriogonum arborescensand Leptosyne gigantea increased significantly (P < 0.0001) in seeded plots only. Nonseeded plots at Smugglers Cove were dominated by exotic annual grasses, primarily Avena barbata. The data indicate that seeding with appropriate native seed is a critical step in restoration following fennel control in areas where the native seed bank is depauperate.

  17. Contact and fumigant activities of constituents of Foeniculum vulgare fruit against three coleopteran stored-product insects.

    Science.gov (United States)

    Kim, D H; Ahn, Y J

    2001-03-01

    The insecticidal activities of materials derived from the fruit of fennel, Foeniculum vulgare, against adults of Sitophilus oryzae, Callosobruchus chinensis and Lasioderma serricorne were examined using direct contact application and fumigation methods. The biologically active constituents of the Foeniculum fruits were characterized as the phenylpropenes (E)-anethole and estragole, and the monoterpene (+)-fenchone, by spectroscopic analysis. Responses varied with insect species, compound, dose and exposure time. In a filter paper diffusion test, estragole at 0.168 mg cm-2 caused 91% mortality to S oryzae adults within 1 day after treatment (DAT), whereas (+)-fenchone and (E)-anethole gave over 90% mortality at 2 and 4 DAT, respectively. Against C chinensis adults, all test compounds revealed potent insecticidal activities at 0.021 mg cm-2 at 2 DAT. Against L serricorne adults at 0.105 mg cm-2, (E)-anethole gave 100% mortality at 1 DAT, whereas 90 and 60% mortality at 4 DAT was achieved with estragole and (+)-fenchone, respectively. In a fumigation test, the compounds were much more effective against adults of S oryzae, C chinensis and L serricorne in closed cups than in open ones, indicating that the insecticidal activity of test compounds was largely attributable to fumigant action. As naturally occurring insect-control agents, the F vulgare fruit-derived materials described could be useful for managing field populations of S oryzae, C chinensis and L serricorne.

  18. Effect of the aqueous extract of Foeniculum vulgare (fennel on the kidney in experimental PCOS female rats

    Directory of Open Access Journals (Sweden)

    Somayyeh Sadrefozalayi

    2014-02-01

    Full Text Available Objective: Foeniculum vulgare seed (F. vulgare is a herbal plant which is used with phytoestrogene compounds for polycystic ovary syndrome (PCOS treatment. In this research, renoprotective effect of the aqueous extract of Foeniculum vulgare (AEF in experimental PCOS female rats is studied. Materials and Methods: Forty female rats were randomly divided into five groups. The first group served as control,was injected with an equivalent volume (0.2 ml of normal saline, and received normal diet. Animals in the second group were non poly cystic ovary syndrome (PCOS rats which were treated with intragastric administration of aqueous extract of F. vulgare (150 mg/kg b.w.. In the third group, the rats were treated with intraperitoneal injection of estradiolvalerate (EV (4 mg in 0.2 ml of sesame oil. The fourth groups were treated with EV and AEF (150mg/kg bw with the same route.  The fifth groups were treated with EV and AEF (100mg/kg bw. After 4 weeks of study, all of the rats were sacrificed, their kidneys tissues were processed for light microscopy, and some biochemical parameters of serum were measured. Results: The mean values of blood urea nitrogen in PCOS rats treated with low dose of AEF and EV and non-treated, was significantly (p

  19. STUDY ON PHARMACOGNOSY OF FOENICULUM VULGARE MILL.AN ETHNODRUG%茴香根的生药学研究

    Institute of Scientific and Technical Information of China (English)

    韦群辉; 朱兆云

    2000-01-01

    目的:为开发利用民族药茴香提供理论依据。方法:采用来源鉴别、性状鉴别、显微及理化鉴别的方法。结果:证明其主要有效成分为挥发油,主要显微特征为木栓层下具有一列红棕色色素细胞层。结论:茴香根是一味具有开发前景的民族药。%To provide the basis for development and utilization of Foeniculum vulgare Mill.METHODS:To study its sources,shapes properties,microscopic and physiochemical characteristics used in pharmacognostic identification.RESULTS:The experiments prove its main effective component is volatile oils and main microscopic featares are that there is a row of light brown cells under the layers of bark.CONCLUSION:Foeniculum vulgare Mill.is worth of development in future  

  20. Chemical Constituents of Foeniculum vulgare Mill.%小茴香根的化学成分研究

    Institute of Scientific and Technical Information of China (English)

    张嫩玲; 马青云; 胡江苗; 周俊; 赵友兴

    2011-01-01

    Five known compounds were isolated from the roots of Foeniculum vulgare Mill.. On the basis of MS and NMR analysis, these compounds were determined as dillapiol ( 1 ), sucrose linoleate (2),3R, 8S,9Z-falcarindiol (3) ,β-sitosterol (4) ,and stigmasteryl-β-D-glueopyranoside (5) ,among which two compounds (2 and 3) were obtained from this plant for the first time.%从小茴香(Foeniculum vulgare Mill.)根的乙醇提取物中分离得到了5个化合物,通过MS和NMR等方法鉴定为:莳萝脑(1),亚油酸蔗糖苷(2),镰叶芹二醇(3),β-谷甾醇(4),豆甾醇-β-D-吡喃葡萄糖苷(5),其中化合物2和3首次从该种植物中分离得到.

  1. Use of gamma radiation cobalt 60 for disinfestation of Lasioderma serricorne (Fabricius, 1972) (Coleoptera: Anobiidae) in Chamomilla recutita L. and Pimpinela anisum L. dehydrated

    Energy Technology Data Exchange (ETDEWEB)

    Alves, Juliana Nazare; Potenza, Marcos Roberto [Instituto Biologico, Sao Paulo, SP (Brazil). Centro de Pesquisa e Desenvolvimento de Sanidade Vegetal]. E-mail: julianaabc@ig.com.br; Arthur, Valter [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil)]. E-mail: arthur@cena.usp.br

    2008-03-15

    Stores products such a grains, flours, dry fruits and spices are normally infested by pests as beetles (Lasioderma serricorne), mites and moths, depreciating the product visually and promoting its deterioration. To improve the quality of spices, medicinal plants and others foodstuffs there is a need for adequate methods of handling, correct identification of the species, adequate collection and storage. The objective of this work was to determine the dose of gamma radiation for the disinfestation of medicinal and aromatic plants infested by L. serricorne. The plants used in this study were Chamomilla recutita L. and Pimpinela anisum L. in this dehydrated form. The experiment was carried out in the Laboratorio de Inseticidas e Acaricidas from Instituto Biologico/SP, during the months of January and May 2006, and the irradiations were carried out in the Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN/SP, using and experimental Cobalt 60 irradiator, model Gammacell 220. Each treatment consisted of 5 parcels containing 10 g of dehydrated products infested with 20 last instar larvae of L. serricorne, conditioned in plastic 10 x 10 cm containers with small punctures in the cover to allow internal aeration. The substratum previously infested was submitted to increasing doses of gamma radiation: 0: 0.5; 0.75; 1.0; 1.25; 1.50; 1.75; 2.0; 2.25; 2.50 and 2.75 kGy. After irradiation, the samples were kept in a acclimatized room at 27 {+-} 2 deg C of temperature and relative humidity of 70 {+-} 5% and after a 45 days period the number of adults insects emerged was evaluated. The disinfestation dose of gamma radiation for last instar larvae L. serricorne on Chamomilla recutita L. and Pimpinela anisum L. was 2.0 kGy. (author)

  2. Distillation time modifies essential oil yield, composition, and antioxidant capacity of fennel (Foeniculum vulgare Mill).

    Science.gov (United States)

    Zheljazkov, Valtcho D; Horgan, Thomas; Astatkie, Tess; Schlegel, Vicki

    2013-01-01

    Fennel (Foeniculum vulgare Mill) is an essential oil crop grown worldwide for production of essential oil, as medicinal or as culinary herb. The essential oil is extracted via steam distillation either from the whole aboveground biomass (herb) or from fennel fruits (seed). The hypothesis of this study was that distillation time (DT) can modify fennel oil yield, composition, and antioxidant capacity of the oil. Therefore, the objective of this study was to evaluate the effect of eight DT (1.25, 2.5, 5, 10, 20, 40, 80, and 160 min) on fennel herb essential oil. Fennel essential oil yield (content) reached a maximum of 0.68% at 160 min DT. The concentration of trans-anethole (32.6-59.4% range in the oil) was low at 1.25 min DT, and increased with an increase of the DT. Alpha-phelandrene (0.9-10.5% range) was the lowest at 1.25 min DT and higher at 10, 80, and 160 min DT. Alpha-pinene (7.1-12.4% range) and beta-pinene (0.95-1.64% range) were higher in the shortest DT and the lowest at 80 min DT. Myrcene (0.93-1.95% range), delta-3-carene (2.1-3.7% range), cis-ocimene (0-0.23% range), and gamma-terpinene (0.22-2.67% range) were the lowest at 1.25 min DT and the highest at 160 min DT. In contrast, the concentrations of paracymene (0.68-5.97% range), fenchone (9.8-22.7% range), camphor (0.21-0.51% range), and cis-anethole (0.14-4.66% range) were highest at shorter DT (1.25-5 min DT) and the lowest at the longer DT (80-160 min DT). Fennel oils from the 20 and 160 min DT had higher antioxidant capacity than the fennel oil obtained at 1.25 min DT. DT can be used to obtain fennel essential oil with differential composition. DT must be reported when reporting essential oil content and composition of fennel essential oil. The results from this study may be used to compare reports in which different DT to extract essential oil from fennel biomass were used.

  3. Phenolic compounds from Foeniculum vulgare (Subsp. Piperitum (Apiaceae herb and evaluation of hepatoprotective antioxidant activity

    Directory of Open Access Journals (Sweden)

    Mona T. M. Ghanem

    2012-01-01

    Full Text Available Objective: The study was designed to evaluate the antioxidant and hepatoprotective activities of the 80% methanolic extract as well as the ethyl acetate (EtOAc and butanol (BuOH fractions of the wild fennel (Foeniculum vulgare (Subsp; Piperitum and cultivated fennel (F. vulgare var. azoricum. In addition, quantification of the total phenolic content in the 80% methanol extract of fennel wild and cultivated herbs is measured. Materials and Methods: An amount of 400 g of air dried powdered herb of wild and cultivated fennel were sonicated with aqueous methanol (80%, successively extracted with Hexane, EtOAc, and n-BuOH. The EtOAc and n-BuOH were subjected to repeated column chromatography on silica gel and Sephadex LH-20. The antioxidant effect was determined in vitro using 1,1-diphenyl-2-picrylhydrazyl (DPPH· . Hepatoprotective activity was carried out using a Wistar male rat (250-300 g. Total phenolic and flavonoid contents were determined as chlorogenic acid and rutin equivalents, respectively. Results: Two phenolic compounds, i.e., 3,4-dihydroxy-phenethylalchohol-6-O-caffeoyl-β-d-glucopyranoside and 3?,8?-binaringenin were isolated from the fennel wild herb, their structures were elucidated by spectral methods including 1D NMR, 2D NMR, and UV. The EtOAc and BuOH fractions of wild fennel were found to exhibit a radical scavenging activity higher than those of cultivated fennel. An in vitro method of rat hepatocytes monolayer culture was used for the investigation of hepatotoxic effects of the 80% methanol extract on the wild and cultivated fennel, which were >1000 and 1000 ΅g/mL, respectively. As well as, their hepatoprotective effect against the toxic effect of paracetamol (25 mM was exerted at 12.5 ΅g/mL concentration. Conclusions: Fennel (F. Vulgare is a widespread plant species commonly used as a spice and flavoring. The results obtained in this study indicated that the fennel (F. vulgare herb is a potential source of natural

  4. Seed germination of medicinal plant, fennel (Foeniculum vulgare Mill), as affected by different priming techniques.

    Science.gov (United States)

    Tahaei, Amirreza; Soleymani, Ali; Shams, Majid

    2016-09-01

    Reduced seed germination is among the most important factors adversely affecting crop stand and subsequent plant growth. Fennel (Foeniculum vulgare Mill) is an important medicinal plant with poor seed germination rate, occasionally. It is accordingly pertinent to find methods which can enhance fennel seed germination and remove the barriers of dormancy breaking. The present experiments studied the effects of two different priming (cold moist stratification and osmopriming) and 14 dormancy breaking techniques (hormonal, osmopriming, biopriming, chemical priming, and hydropriming) on the seed germination and seedling growth of two different fennel genotypes under growth chamber conditions. In the first and second experiment, the priming techniques including the time lengths of cold moist stratification (0, 15, 30, and 45 days) and the concentrations of polyethylene glycol 6000 (PEG6000, osmopriming at -0.99, -1.35, and -2.33 MPa) were used as the main plots. However, in both experiments, the dormancy breaking techniques and fennel genotypes were factorially combined and used as the subplots. Different seed- and seedling-related parameters including germination (%), plumule, radicle and seedling length, average germination time, rate and homogeneity of germination, and seed vigor index were determined. Both priming techniques were efficient on the enhancement of seed germination and seedling growth. Among the dormancy breaking techniques, Aminol Forte (biopriming), kadostim (biopriming), benzyl adenine + kinetin (biopriming), distilled water (hydropriming), gibberellin + kinetin (hormonal priming), and benzyl adenine + kinetin + gibberellin (biopriming) were the most effective ones. The related concentrations were equal to 100 mg/l, 10(-5) M, and 0.4 %. The fennel genotypes reacted significantly different under priming conditions. It is possible to enhance seed germination and seedling growth of fennel using priming and dormancy breaking

  5. 细叶黄皮各器官挥发油的化学成分%Chemical Constituents of Essential Oil of Different Organs from Clausena anisum-olens

    Institute of Scientific and Technical Information of China (English)

    苏秀芳; 梁振益

    2011-01-01

    Objective: To analyze the chemical constituents of essential oil from the plant of Clausena anisum-olens. Method: The essential oil was extracted from C. anisum-olens by steam distillation and analyzed by GC-MS. Result: Essential oil from stems, roots, leaves, fruits and flowers contain 4-methoxy-6-( 2-propenyl)-1, 3-benzodioxole,which respective accounted of the essential oil for 32. 89% , 38. 09%, 28. 97% , 58. 40% and 100.00%. The main types of the compounds are aromatic hydrocarbons, alkane,olefin and fatty acids. Conclusion: The essential oil of different organs of C. anisum-olens is studied. It provides scientific bases for the C. anisum-olens exploitation and usage.%目的:分析研究细叶黄皮植物挥发油的化学成分.方法:采用水蒸气蒸馏法从细叶黄皮各器官中提取挥发油,用气相色谱-质谱联用技术对挥发油化学成分进行分析.结果:细叶黄皮茎、根、叶、果实及花挥发油的共有化合物为4-甲氧基-6-(2-丙烯基)-1,3-苯并二恶茂,质量分数分别为32.89%,38.09%,28.97%,58.40%,100.00%.化合物的主要类型为芳香类、烯类、烷烃类及酸类.结论:研究了细叶黄皮各器官挥发油的化学成分,为合理开发和利用细叶黄皮资源提供了依据.

  6. Comparative essential oil composition and antifungal effect of bitter fennel (Foeniculum vulgare ssp. piperitum) fruit oils obtained during different vegetation.

    Science.gov (United States)

    Ozcan, Mehmet Musa; Chalchat, Jean-Claude; Arslan, Derya; Ateş, Ayşe; Unver, Ahmet

    2006-01-01

    The chemical composition of the flower and unripe and ripe fruits from fennel (bitter) (Foeniculum vulgare ssp. piperitum) has been examined by gas chromatography and gas chromatography-mass spectrometry. The main identified components of the flower and unripe and ripe fruit oils were estragole (53.08%, 56.11%, and 61.08%), fenchone (13.53%, 19.18%, and 23.46%), and alpha-phellandrene (5.77%, 3.30%, and 0.72%), respectively. Minor qualitative and major quantitative variations for some compounds of essential oils were determined with respect to the different parts of F. vulgare. The oils exerted varying levels of antifungal effects on the experimental mycelial growth of Alternaria alternata, Fusarium oxysporum, and Rhizoctonia solani. The 40 ppm concentrations of fennel oils showed inhibitory effect against mycelial growth of A. alternaria, whereas 10 ppm levels were ineffective. The analyses show that fennel oils exhibited different degrees of fungistatic activity depending on the doses.

  7. Near-infrared analysis of fennel (Foeniculum vulgare Miller) on different spectrometers--basic considerations for a reliable network.

    Science.gov (United States)

    Steuer, Boris; Schulz, Hartwig

    2003-01-01

    The aim of this study was to investigate the accuracy and transferability of near-infrared (NIR) calibrations for estimating the content and composition of the volatile fraction in fennel fruits (Foeniculum vulgare Miller) as an example of medicinal and spice plants. A master calibration with spectra obtained on a scanning monochromator was generated using 345 samples from three different harvests (1997-1999). A subset of 70 samples from 1999 was also measured on a dispersive grating and a scanning diode array system to gain an insight into the influence of sample presentation and scanning techniques. For all instruments, calibrations with standard errors in the range of the reference method were achieved. Furthermore the influence of storage on NIR spectra and, additionally, the potential of transferring spectra between both scanning monochromators was studied.

  8. Investigation of the cytotoxic, genotoxic, and apoptosis-inducing effects of estragole isolated from fennel (Foeniculum vulgare).

    Science.gov (United States)

    Villarini, Milena; Pagiotti, Rita; Dominici, Luca; Fatigoni, Cristina; Vannini, Samuele; Levorato, Sara; Moretti, Massimo

    2014-04-25

    The present study was undertaken to evaluate, in the HepG2 human hepatoma cell line, the in vitro cytotoxic, genotoxic, and apoptotic activities of estragole (1), contained in the essential oil of Foeniculum vulgare (fennel) and suspected to induce hepatic tumors in susceptible strains of mice. Toward this end, an MTT cytotoxicity assay, a trypan blue dye exclusion test, a double-staining (acridine orange and DAPI) fluorescence viability assay, a single-cell microgel-electrophoresis (comet) assay, a mitochondrial membrane potential (Δψm) assay, and a DNA fragmentation analysis were conducted. In terms of potential genotoxic effects, the comet assay indicated that estragole (1) was not able to induce DNA damage nor apoptosis under the experimental conditions used.

  9. Inhibition of aldose reductase and anti-cataract action of trans-anethole isolated from Foeniculum vulgare Mill. fruits.

    Science.gov (United States)

    Dongare, Vandana; Kulkarni, Chaitanya; Kondawar, Manish; Magdum, Chandrakant; Haldavnekar, Vivek; Arvindekar, Akalpita

    2012-05-01

    Foeniculum vulgare fruits are routinely consumed for their carminative and mouth freshening effect. The plant was evaluated for aldose reductase inhibition and anti-diabetic action. Bioguided fractionation using silica gel column chromatography, HPLC, and GC-MS analysis revealed trans-anethole as the bioactive constituent possessing potent aldose reductase inhibitory action, with an IC50 value of 3.8μg/ml. Prolonged treatment with the pet ether fraction of the F. vulgare distillate demonstrated improvement in blood glucose, lipid profile, glycated haemoglobin and other parameters in streptozotocin-induced diabetic rats. Trans-anethole could effectively show anti-cataract activity through the increase in soluble lens protein, reduced glutathione, catalase and SOD activity on in vitro incubation of the eye lens with 55mM glucose. Trans-anethole demonstrated noncompetitive to mixed type of inhibition of lens aldose reductase using Lineweaver Burk plot.

  10. Studies on gamma irradiated medicinal plants and spices (1): Myristica argentea, Myristica fragrans, Coriandrum sativum and Foeniculum vulgare

    Energy Technology Data Exchange (ETDEWEB)

    Hilmy, N.; Chosdu, R.; Sudiro, S. (National Atomic Energy Agency, Jakartc (Indonesia). Pasar Djumat Research Centre); Syuib, F. (Bandung Inst. of Tech. (Indonesia). Pharmacy Dept.)

    1981-10-01

    Medicinal plants i.e. dried seeds of myristica argentea, myristica fragrans, coriandrum sativum and foeniculum vulgare were stored for 0 and 6 months, with and without gamma irradiation at 5 kGy. No detectable changes were seen in infrared and UV spectra, GLC chromatograms and refraction indices of steam distilled, essential oils from the irradiated medicinal plants. The same results were also obtained from irradiated samples stored for 6 months at ambient conditions. Storage for 6 months caused significant changes in the gas liquid chromatograms and UV spectra of all samples compared to non-stored samples. The moisture content of all samples packed in polyethylene bags seems to be constant after 6 months of storage. No measurable changes were found in the amount of essential oil content caused by irradiation.

  11. Extraction and characterization of Foeniculum vulgare pectins and their use for preparing biopolymer films in the presence of phaseolin protein.

    Science.gov (United States)

    Giosafatto, Concetta V L; Mariniello, Loredana; Ring, Steve

    2007-02-21

    Pectins from Foeniculum vulgare were extracted under acidic conditions. The obtained pectins were mainly composed of uronic acid but also contained traces of rhamnose, galactose, and arabinose. Extracted pectins were used as a carbohydrate source to prepare biopolymer films in the absence and in the presence of phaseolin protein. The swelling characteristics of the films were examined as a function of ionic strength, pH, and the applied osmotic stress. The swelling behavior was dominated by a Donnan-type effect, which decreases with increasing ionic strength and counterion valency. In all cases the swelling of films containing phaseolin was reduced, suggesting a network formation between protein and pectins. Mechanical property studies have also estimated the validity of the obtained novel biopolymer films in terms of mechanical resistance.

  12. Chemical Constituents of the Root of Foeniculum vulgare%茴香根化学成分研究

    Institute of Scientific and Technical Information of China (English)

    武拉斌; 黄波; 肖朝江; 董相; 姜北

    2013-01-01

    Objective:To explore the chemical constituents of the root of Foeniculum vulgare. Methods: Compounds were separated and purified by silica gel column chromatography and their structures were elucidated on the basis of spectral analysis and chemical evidences. Results: Five compounds were obtained from ethyl acetate fraction of the extract root of F. vulgare and were identified as dillapiol (1), butyl isobutyl phthalate (2), β-sitosterol (3), linoleic acid (4), and trilinolein (5). Conclusion: This research provides basic understanding about the medicinal values of F. vulgare root.%目的:研究茴香(Foeniculum vulgare)根的化学成分。方法:采用硅胶柱层析等分离方法对茴香根提取物中的化学成分进行分离,并运用NMR、MS等波谱学技术对所分离得到的化合物进行结构解析。结果:从茴香干燥根70%乙醇提取物中分离鉴定了5个化合物:莳萝脑(1)、邻苯二甲酸丁基异丁基二酯(2)、β-谷甾醇(3)、亚油酸(4)、亚油酸甘油三酯(5)。结论:该研究为了解茴香根的药用价值提供了研究基础。

  13. Sazonalidade dos ductos secretores e óleo essencial de Foeniculum vulgare var. vulgare Mill. (Apiaceae Seasonality of the secretory ducts and essential oil of Foeniculum vulgare var. vulgare Mill. (Apiaceae

    Directory of Open Access Journals (Sweden)

    L.A. Sousa

    2005-06-01

    Full Text Available Este trabalho teve como objetivo analisar os ductos secretores e o óleo essencial das folhas de Foeniculum vulgare em diferentes épocas do ano. Para esta finalidade, foram realizados estudos de caracterização anatômica, bem como anatomia comparada dos ductos secretores e testes histoquímicos das folhas. O óleo essencial foi obtido de folhas e frutos, por hidrodestilação em aparelho de Clevenger e analisados quantitativamente e qualitativamente por cromatografia em fase gasosa acoplada ao espectrômetro de massa, realizando-se análises seguidas de três réplicas para folhas coletadas durante o inverno e primavera, e frutos no verão. Os resultados encontrados para os ductos secretores de óleo corresponderam à redução do teor de óleo essencial nas folhas coletadas no final da primavera. O componente majoritário do óleo essencial de folhas e frutos foi o trans-anetol, durante todas as estações do ano. Portanto, evidenciou-se que os ductos secretores e teor de óleo essencial estão relacionados, bem como os constituintes químicos também estão sujeitos a sazonalidade, conforme o estágio fenológico da planta.This work aimed to analyze the secretory ducts and the essential oil of the leaves of Foeniculum vulgare in different periods of the year. For this purpose, the anatomic characterization, and histochemical tests of the leaves were performed as well as the comparative anatomy of the secretory ducts. The essential oil was obtained from the leaves and fruits by hidrodistillation in a Clevenger-type apparatus and analyzed by gas chromatography coupled to a mass spectrometer, each analysis was repeated three times for leaves collected during the winter and spring, and for fruits collected in the Summer. The results obtained for the secretory ducts, corresponded to the yield reduction of essential oil in the leaves collected in the end of the spring. The major substance of the essential oil of the leaves and fruits, in all

  14. 宁夏海原小茴香发展现状及前景展望%Development Status and Prospect of Foeniculum vulgare Mill.in Haiyuan of Ningxia

    Institute of Scientific and Technical Information of China (English)

    雷茜; 张欣; 贝盏临; 吕云熙

    2012-01-01

    The paper introduced the distribution, biological characteristics, chemical composition and industry status of Foeniculum vulgare Mill, in Ningxia, analyzed the main issues restricting the development of Haiyuan Foeniculum vulgare Mill. , and finally forecasted the development potential and prospect of Haiyuan Foeniculum vulgare Mill, industry in future.%介绍了小茴香在宁夏的分布、生物学特性、化学成分及其产业现状;分析了目前制约海原小茴香发展的主要问题;展望了未来海原小茴香产业发展的潜力及前景.

  15. Contact Toxicity and Repellency of the Main Components From the Essential Oil of Clausena anisum-olens Against Two Stored Product Insects.

    Science.gov (United States)

    You, Chun Xue; Jiang, Hai Yan; Zhang, Wen Juan; Guo, Shan Shan; Yang, Kai; Lei, Ning; Ma, Ping; Geng, Zhu Feng; Du, Shu Shan

    2015-01-01

    The essential oil of Clausena anisum-olens (Blanco) Merr. showed strong contact toxicity and repellency against Lasioderma serricorne and Liposcelis bostrychophila adults. The components of the essential oil obtained by hydrodistillation were determined by gas chromatography-mass spectrometry. It was found that the main components were myristicin (36.87%), terpinolene (13.26%), p-cymene-8-ol (12.38%), and 3-carene (3.88%). Myristicin and p-cymene-8-ol were separated by silica gel column chromatography, and their molecular structures were confirmed by means of physicochemical and spectrometric analysis. Myristicin and p-cymene-8-ol showed strong contact toxicity against L. serricorne (LD50 = 18.96 and 39.68 μg per adult) and Li. bostrychophila (LD50 = 20.41 and 35.66 μg per adult). The essential oil acting against the two grain storage insects showed LD50 values of 12.44 and 74.46 μg per adult, respectively. Myristicin and p-cymene-8-ol have strong repellent toxicity to Li. bostrychophila.

  16. 茴香茎叶提取物对菜粉蝶生物活性的影响研究%Preliminary Studies on Biological Activity of Extracts from Foeniculum vulgare to Pieris rapae L

    Institute of Scientific and Technical Information of China (English)

    吴利民; 陆宁海; 蒋国锋; 魏旺; 蒋坤; 李德兵

    2012-01-01

    为了探索番茄茎叶提取物对菜粉蝶生物活性的影响.采用水蒸气蒸馏法从茴香( Foeniculum vulgare)茎叶中提取获得其挥发性物质,测定了茴香提取物在室内对菜青虫的拒食作用及对菜粉蝶的产卵忌避作用.结果表明:茴香粗提取物对菜青虫3龄幼虫具有较强的拒食活性,且非选择性的拒食作用均大于选择性的拒食作用;茴香提取物对菜粉蝶的产卵具有较强的忌避作用.%This paper objective was to explore biological activity of extracts from Foeniculum vulgare to Pieris rapae. Extract volatile matter was extracted from stem and leaves of Foeniculum vulgare Mill by steam distillation. Biologial activity of antifeedant effect and oviposition repellent on Pieris rapae L. Was tested with extraction of Foeniculum vulgare. The results showed that extracts of Foeniculum vulgare have strong antifeedant effect on Pieris rapae 3-instar larvae, which the antifeeding activities of Pieris rapae L. Of non-choice was higher than that of choice tests. Moreover, that extracts of Foeniculum vulgare had strong effect of repellent effect on Pieris rapae.

  17. Effect of cycocel on photosynthetic activity and essential oil of fennel (Foeniculum vulgare Mill. under drought stress

    Directory of Open Access Journals (Sweden)

    Fatemeh Nouri

    2014-12-01

    Full Text Available Drought stress is one of the most important and most common environmental stresses that limit plant growth. Photosynthesis is the main determinant of plant growth and its retention ability under environmental stress condition is important for preservation of growth stability. To study the effect of spraying CCC on photosynthesis activity and essential oil content on 'Foeniculum vulgare', an experiment was done in split plot design based on complete block with four replications in research field of University of Zanjan in 2011. Levels of drought stress included, control, soft stress (when 30% of available water was out of soil, severe stress (when 90% of available water was out of soil, and three concentration of CCC 0, 1500 and 3000 mg/L. The results showed that between rates foliar application on physiologic characteristics as photosynthesis rate, transpiration severity, RWC and TΔ intercellular (Ci, stomata conductivity, mesophyll conductivity and essential oil content was significant. By spraying 3000 mg/L CCC, balanced the plant position against drought stress and could reduce negative effect. Foliar application of CCC caused significant increase in photosynthesis rate, mesophyll conductivity and significant decrease stomata conductivity, transpiration severity under drought stress and could increase essential oil content under soft stress. In this study, foliar application of CCC to content 3000 mg/L had the highest effect on this characters.

  18. Study of Foeniculum vulgare (Fennel Seed Extract Effects on Serum Level of Estrogen, Progesterone and Prolactin in Mouse

    Directory of Open Access Journals (Sweden)

    Sadeghpour Nahid

    2015-01-01

    Full Text Available Objective: The Foeniculum vulgare (FVE or fennel has a long history of use as both a food and medicine. The seed of this plant has been used to promote menstruation, alleviate the symptoms of female climacteric, and increase the number of ovarian follicles. The aim of this study is to evaluate the fennel extract effects on serum level of estrogen, progesterone and prolactin in female mice. Materials and Methods: A total of 28 virgin female albino mice were divided into four groups (n = 7. Groups 1 and 2 (experimental groups were administered FVE at 100 and at a concentration of 100 and 200 mg/kg for 5 days, intraperitoneally. Group 3 (negative control received ethanol and Group 4 (positive control received normal saline. Animals were scarified at 6th day, sera were collected and the level of estrogen, progesterone, and prolactin hormones was analyzed using Elisa Kit. Results: Data analysis revealed that there is a significant difference in the mean level of serum estrogen, progesterone and prolactin between four different groups. P value in experimental groups compared with the control groups was (P < 0.0001. Conclusion: Fennel extract can increase the serum level of estrogen, progesterone, and prolactin in female mice; it can be introduced as a novel medicine for treatment of infertility

  19. Supercritical CO₂ extraction of volatile oils from Sardinian Foeniculum vulgare ssp. vulgare (Apiaceae): chemical composition and biological activity.

    Science.gov (United States)

    Piras, Alessandra; Falconieri, Danilo; Porcedda, Silvia; Marongiu, Bruno; Gonçalves, Maria José; Cavaleiro, Carlos; Salgueiro, Ligia

    2014-01-01

    This article reports the results on the composition and antifungal effect of volatile extracts obtained from the aerial parts of Sardinian wild fennel (Foeniculum vulgare Mill.), by supercritical fluid extraction (SFE) and by hydrodistillation (HD). The extracts were analysed by gas chromatography-mass spectrometry for qualitative composition and gas chromatography-flame ionisation detector to establish the percentage of constituents. The main components were fenchone (7.1% vs. 8.8%), estragole (34.9% vs. 42.6%) and (E)-anethole (24.6% vs. 43.4%) in the SFE and HD extract, respectively. Minimum inhibitory concentrations (MICs) were measured according to the reference Clinical and Laboratory Standards Institute (CLSI) broth macrodilution protocols. Minimum lethal concentrations were determined by subsequent subculturing of the same cell suspensions in solid medium. The essential oil was more active against Candida albicans, whereas the supercritical fluid extract possesses higher activity against Candida guillermondii and Cryptococcus neoformans, with MIC values of 0.32 μL/mL.

  20. Outbreak of fatal nitrate toxicosis associated with consumption of fennels (Foeniculum vulgare) in cattle farmed in Campania region (southern Italy).

    Science.gov (United States)

    Costagliola, Alessandro; Roperto, Franco; Benedetto, Domenico; Anastasio, Aniello; Marrone, Raffaele; Perillo, Antonella; Russo, Valeria; Papparella, Serenella; Paciello, Orlando

    2014-05-01

    Nitrate and nitrite are toxicants that have become increasingly significant environmental chemicals. Increase in environmental distribution of nitrogenous compounds, especially in surface and ground water, has been attributed to the intensive use of nitrate as agricultural fertilizers and to increasing amounts of nitrogenous wastes produced by municipalities, industries, and feedlots. The purpose of this study is to illustrate a fatal nitrate toxicosis in cattle associated with the consumption of fennels (Foeniculum vulgare). Fifteen cows from the same farm suddenly developed weakness, muscular tremors, respiratory distress, and finally convulsions. The affected animals died within 24 to 48 h from the onset of the clinical signs. Five cows underwent a complete post-mortem examination. In all examined animals, gross lesions included presence of dark unclotted blood around the nostrils and the anal region, moderate inflammation of the gastrointestinal mucosa, and brown discoloration of the skeletal muscles and kidneys. The histological examination showed tubular degeneration and congestion of glomerular vessels in the kidney. Toxicological analysis detected nitrates at 4 672.2 ppm in the fennels used to feed the animals. The source of exposure to nitrates was identified in the fennels. The fennels were grown in a polluted area of the Campania region in southern Italy and distributed in a public market for human consumption. The waste from the sale of the fennels was fed to the cows. The accumulation of nitrates in some vegetables poses a risk not only for animal health but also for human and environmental safety.

  1. Investigation of antimutagenic potential of Foeniculum vulgare essential oil on cyclophosphamide induced genotoxicity and oxidative stress in mice.

    Science.gov (United States)

    Tripathi, Pankaj; Tripathi, Rina; Patel, Rakesh K; Pancholi, Shyam S

    2013-01-01

    The present study investigated the protective effects of Foeniculum vulgare (fennel) essential oil (FEO) against genotoxicity induced by cyclophosphamide (CP). Mice bone marrow chromosomal aberration (CA), micronucleus, and sperm abnormality assays were employed to measure genotoxicity and cytotoxicity, respectively. The activities of superoxide dismutase (SOD), glutathione (GSH), catalase (CAT), and malondialdehyde (MDA) content in the liver were also investigated spectrophotometrically. Animals were administered two different doses of FEO (1 and 2 mL/kg) continuously for 3 days at intervals of 24 hours by the oral route before tissue sampling. The results showed that CP produced a significant increase in the average percentage of aberrant metaphases and CAs, excluding gap and micronuclei formation in polychromatic erythrocytes (PCEs), produced cytotoxicity in mouse bone marrow cells, and induced abnormal sperms in the male germ line. CP also markedly inhibited the activities of SOD, CAT, and GSH and increased MDA content. Pretreatments with FEO significantly inhibited the frequencies of aberrant metaphases, CAs, micronuclei formation, and cytotoxicity in mouse bone marrow cells induced by CP and also produced a significant reduction of abnormal sperm and antagonized the reduction of CP-induced SOD, CAT, and GSH activities and inhibited increased MDA content in the liver. FEO inhibits genotoxicity and oxidative stress induced by CP.

  2. Potent inhibitory effect of Foeniculum vulgare Miller extract on osteoclast differentiation and ovariectomy-induced bone loss.

    Science.gov (United States)

    Kim, Tae-Ho; Kim, Hyun-Ju; Lee, Sang-Han; Kim, Shin-Yoon

    2012-06-01

    Inhibition of osteoclast differentiation and bone resorption is considered an effective therapeutic approach to the treatment of postmenopausal bone loss. To find natural compounds that may inhibit osteoclastogenesis, we screened herbal extracts on bone marrow cultures. In this study, we found that an aqueous extract of Foeniculum vulgare Miller seed (FvMs) at low concentration, which has traditionally been used as a treatment for a variety of ailments, inhibits the osteoclast differentiation and bone resorptive activity of mature osteoclasts. We further investigated the effects of FvMs on ovariectomy (OVX)-induced bone loss using microcomputed tomography, biomechanical tests and serum marker assays for bone remodeling. Oral administration of FvMs (30 mg or 100 mg/kg/day) for 6 weeks had an intermediary effect on the prevention of femoral bone mineral density (BMD), bone mineral content (BMC), and other parameters compared to OVX controls. In addition, FvMs slightly decreased bone turnover markers that were accelerated by OVX. The bone-protective effects of FvMs may be due to suppression of an OVX-induced increase in bone turnover. Collectively, our findings indicate that FvMs have potential in preventing bone loss in postmenopausal osteoporosis by reducing both osteoclast differentiation and function.

  3. Effect of planting date and spacing on growth and yield of fennel (Foeniculum vulgare Mill.) under irrigated conditions.

    Science.gov (United States)

    Al-Dalain, Saddam Aref; Abdel-Ghani, Adel H; Al-Dala'een, Jawad A; Thalaen, Haditha A

    2012-12-01

    Fennel (Foeniculum vulgare Mill.) plant is a medicinal aromatic herb and belongs to Apiaceae family. It has a rich nutritional value and has many medicinal usages. Very limited information is available in the literature about fennel cultivation and production practices. Therefore, this study was carried out to evaluate the effect of planting date and plant spacing and their interactive effects on yield, yield components and growth of Fennel under irrigation. Three planting dates (Oct. 1st, Nov. 1st and Dec. 1st) and four plant spacings (10, 20, 30 and 40 cm with constant row width, 60 cm) were used. Fruit yield was significantly (p<0.05) influenced by plant spacing and planting date and their interaction. Early planting significantly increased the fruit yield combined with higher number of branches per plant, number of umbrella per plant, number of fruit per plant and plant height. The percentage of increases in Oct. 1st were 34.4 and 32.2% in fruit and biological yield respectively compared with Dec. 1st. Harvest index and thousand fruit weight was not significantly affected by planting date. Increase plant spacing to 30 cm led to more than 15% increase in fruit and biological yield. The early planting date with 30 cm plant spacing resulted in higher fruit (4136 kg ha(-1)) and biological yield (10,114 kg ha(-1)).

  4. Antifungal efficacy of Punica granatum, Acacia nilotica, Cuminum cyminum and Foeniculum vulgare on Candida albicans: An in vitro study

    Directory of Open Access Journals (Sweden)

    Pai Mithun

    2010-01-01

    Full Text Available Background: The establishment and maintenance of oral microbiota is related not only to interbacterial coaggregations but also to interactions of these bacteria with yeasts. Hence, it is important for agents used in the treatment of oral diseases to have antifungal properties for effective therapy. Objective: The main purpose of this study was to evaluate the in vitro antifungal efficacy of Punica granatum, Acacia nilotica, Cuminum cyminum and Foeniculum vulgare on Candida albicans. Materials and Methods: The pomegranate peel is separated, dried and powdered. Fennel, cumin and acacia bark obtained from the tree are powdered. Candida is inoculated at 37˚C and seeded on Sabourauds agar medium. Sterilized filter papers saturated with 30 μl of the extracts are placed on the seeded plates and inoculated at 24 and 48 h. Zones of inhibition on all four sides are measured around the filter paper with a vernier caliper. The experiments were repeated on four plates, with four samples of each extract on one plate for all of the extracts. Results: All the above-mentioned ingredients showed antifungal property, with Punica granatum showing the highest inhibition of Candida albicans with a mean zone of inhibition of 22 mm. P-values <0.05 were obtained for Punica granatum when compared with the other extracts. Conclusion: The results showed the potential use of these products as cheap and convenient adjuvants to pharmaceutical antifungal products.

  5. Empirical prediction and validation of antibacterial inhibitory effects of various plant essential oils on common pathogenic bacteria.

    Science.gov (United States)

    Akdemir Evrendilek, Gulsun

    2015-06-02

    In this study, fractional compound composition, antioxidant capacity, and phenolic substance content of 14 plant essential oils-anise (Pimpinella anisum), bay leaves (Laurus nobilis), cinnamon bark (Cinnamomum verum), clove (Eugenia caryophyllata), fennel (Foeniculum vulgare), hop (Humulus lupulus), Istanbul oregano (Origanum vulgare subsp. hirtum), Izmir oregano (Origanum onites), mint (Mentha piperita), myrtus (Myrtus communis), orange peel (Citrus sinensis), sage (Salvia officinalis), thyme (Thymbra spicata), and Turkish oregano (Origanum minutiflorum)--were related to inhibition of 10 bacteria through multiple linear or non-linear (M(N)LR) models-four Gram-positive bacteria of Listeria innocua, coagulase-negative staphylococci, Staphylococcus aureus, and Bacillus subtilis, and six Gram-negative bacteria of Yersinia enterocolitica, Salmonella Enteritidis, Salmonella Typhimurium, Proteus mirabilis, Escherichia coli O157:H7, and Klebsiella oxytoca. A total of 65 compounds with different antioxidant capacity, phenolic substance content and antibacterial properties were detected with 14 plant essential oils. The best-fit M(N)LR models indicated that relative to anise essential oil, the essential oils of oreganos, cinnamon, and thyme had consistently high inhibitory effects, while orange peel essential oil had consistently a low inhibitory effect. Regression analysis indicated that beta-bisabolene (Turkish and Istanbul oreganos), and terpinolene (thyme) were found to be the most inhibitory compounds regardless of the bacteria type tested. Copyright © 2015 Elsevier B.V. All rights reserved.

  6. An attempt of postharvest orange fruit rot control using essential oils from Mediterranean plants.

    Science.gov (United States)

    Camele, Ippolito; De Feo, Vincenzo; Altieri, Luciana; Mancini, Emilia; De Martino, Laura; Luigi Rana, Gian

    2010-12-01

    Twelve essential oils from Mediterranean aromatic plants were tested at different doses against four fungi known as causal agents of post-harvest orange fruit rot: Botrytis cinerea, Penicillium italicum, Phytophthora citrophthora, and Rhizopus stolonifer. Essential oils were obtained from Hyssopus officinalis, Lavandula angustifolia, Majorana hortensis, Melissa officinalis, Ocimum basilicum, Origanum vulgare, Salvia officinalis, and Thymus vulgaris (Family Lamiaceae), Verbena officinalis (Family Verbenaceae), and Pimpinella anisum, Foeniculum vulgare, and Carum carvi (Family Apiaceae). Because preliminary in vitro experiments showed that only the oils from V. officinalis, T. vulgaris, and O. vulgare exhibited some fungistatic activity against the above-named fungi, these three essential oils were used in successive in vivo tests carried out to protect healthy "Washington navel" orange fruits from artificial infection by the same micromycetes. The essential oil of T. vulgaris, at a 2,000 ppm dose, controlled fruit rot by B. cinerea, P. citrophthora, and R. stolonifer but was ineffective against P. italicum. Essential oils of V. officinalis and O. vulgare inhibited infection by the first two fungi and only by P. citrophthora, respectively. This finding represents an important result, with the goal of using the essential oils as natural preservatives for food products, due to their positive effect on their safety and shelf life.

  7. Apiaceae seeds as functional food

    Directory of Open Access Journals (Sweden)

    Aćimović Milica G.

    2015-01-01

    Full Text Available The aim of this review was to point to a great importance of plants from Apiaceae family as a functional food. Caraway (Carum carvi L., anise (Pimpinella anisum L., coriander (Coriandrum sativum L., dill (Anethum graveolens L., fennel (Foeniculum vulgare Mill. and cumin (Cuminum cyminum L. are plants from the above-mentioned family whose seeds are widely used in folk medicine, pharmaceutical industry, as spices, flavoring agents and as dietary supplements. These plants are rich in essential oil, which is a mixture of volatile compounds that give it a characteristic aroma. Their antioxidant and antimicrobial activities have been proven and because of these activities they have great potential to be used as natural food conservatives. These plants also have hypoglycemic and hypolipidemic activities as well as anticancer properties. They are used as food supplements in everyday nutrition and as natural health products for the prevention and treatment of many disorders such as inflammations, hyperglycemia, hyperlipidemia and others. Apart from this, these plants have real application in foods such as pastries, meat and dairy products, pickles and salads as well as spice blends like curry powder, garam masala and others.

  8. 小茴香挥发油的抗炎镇痛作用%Anti-inflammatory and Analgesic Effects of Volatile Oil Extracted from Foeniculum vulgare Mill. Seeds

    Institute of Scientific and Technical Information of China (English)

    滕光寿; 刘曼玲; 毛峰峰; 韩燕; 杨鹏; 石磊; 畅敏

    2011-01-01

    Objective: To study the efects of anti-inflammatory and analgesic of volatile oil extracted from Foeniculum vulgare Mill.Seeds and offer pharmacological and experimental basis for its safe and effective use in clinic.Methods: The pharmacodynamic effects were obsersed in three experimental models of inflammation and pain: (l)mouse auricular edema induced by xylen; (2) Feet tumefaction caused by albumen in rats; (3)writhing induced by acetic acid in mice.Results: Volatile oil from Foeniculum vulgare Mill.Seeds could distinctively inhibit the inflammatory edema caused by various inflammatory agents and reduce the times of writhe induced by acetic acid in mice.Conclusion: Volatile oil from Foeniculum vulgare Mill.Seeds has analgesic effect and could inhibit inflammation in animals.%目的:研究小茴香挥发油的抗炎、镇痛作用,为指导临床合理用药提供科学依据.方法:应用二甲苯致小鼠耳廓肿胀、蛋清致大鼠足肿胀2种动物模型进行抗炎药效学实验;采用醋酸致小鼠扭体反应进行镇痛实验.结果:小茴香挥发油能显著抑制上述各种动物模型的炎症反应及醋酸引起的小鼠扭体反应.结论:小茴香挥发油具有抗炎和镇痛作用.

  9. AVALIAÇÃO DA QUALIDADE DOS FRUTOS DE FUNCHO (Foeniculum vulgare Mill. UTILIZADOS NO PREPARO DE CHÁS

    Directory of Open Access Journals (Sweden)

    Renato Farina Menegon

    2013-09-01

    Full Text Available A espécie Foeniculum vulgare Mill. conhecida popularmente como funcho ou falsa erva-doce, apresenta óleos essenciais em sua composição, onde está contido o princípio ativo que caracteriza a ação estomática, diurética, carminativa, anti-inflamatória, bactericida e espasmolítica da planta. Por esses efeitos, pelo seu fácil acesso à população e pelo baixo custo, foi verificado que seu consumo vem aumentando significativamente entre a população e, diante disso, observa-se a necessidade de intensificar a fiscalização quanto ao controle da qualidade desses chás, objetivando maior segurança no uso e maior garantia dos efeitos desejados. A qualidade de amostras de funcho, comercializadas na cidade de São José dos Campos, foi avaliada por determinação de material estranho, determinação do teor de óleos essenciais, determinação de umidade e cinzas totais, além da análise das informações contidas nos rótulos. Para esses testes, foram adquiridas 5 marcas comerciais diferentes da erva, comercializadas em diferentes estabelecimentos da cidade. Parte das amostras analisadas estava em desacordo com a legislação vigente e com a Farmacopéia Brasileira 5ª Ed., destacando as falhas quanto ao controle de qualidade desses produtos em todos os aspectos analisados.

  10. Effect of Cymbopogon martinii, Foeniculum vulgare, and Trachyspermum ammi Essential Oils on the Growth and Mycotoxins Production by Aspergillus Species.

    Science.gov (United States)

    Gemeda, Negero; Woldeamanuel, Yimtubezinash; Asrat, Daniel; Debella, Asfaw

    2014-01-01

    This study was performed to investigate effect of essential oils on Aspergillus spore germination, growth, and mycotoxin production. In vitro antifungal and antiaflatoxigenic activities of Cymbopogon martinii, Foeniculum vulgare, and Trachyspermum ammi essential oils were carried out on toxigenic strains of Aspergillus species. Plant materials were hydrodistilled for 4-5 h in Clevenger apparatus. 0.25 μL/mL, 0.5 μL/mL, 1 μL/mL, 2 μL/mL, and 4 μL/mL concentrations of each essential oil were prepared in 0.1% Tween 80 (V/V). T. ammi oil showed highest antifungal activity. Absolute mycelial inhibition was recorded at 1 μL/mL by essential oils of T. ammi. The oil also showed complete inhibition of spore germination at a concentration of 2 μL/mL. In addition, T. ammi oil showed significant antiaflatoxigenic potency by totally inhibiting toxin production from A. niger and A. flavus at 0.5 and 0.75 μL/mL, respectively. C. martinii, F. vulgare, and T. ammi oils as antifungals were found superior over synthetic preservative. Moreover, a concentration of 5336.297 μL/kg body weight was recorded for LC50 on mice indicating the low mammalian toxicity. In conclusion, the essential oils from T. ammi can be a potential source of safe natural food preservative for food commodities contamination by Aspergillus species.

  11. Comparison of Scolicidal Effects of Amphotricin B, Silver Nanoparticles,_and Foeniculum vulgare Mill on Hydatid Cysts Protoscoleces.

    Directory of Open Access Journals (Sweden)

    Mohammad Reza Lashkarizadeh

    2015-06-01

    Full Text Available Surgery is the preferred treatment for hydatid cyst (cystic echinococcosis, CE. At present, various scolicidal agents have been used for inactivation of protoscoleces during surgery, but they are associated with adverse side effects. The aim of the present study was to evaluate the scolicidal effects of amphotricin B, Silver nano particles, Foeniculum vulgare Mill, essential oil and hypertonic saline against protoscoleces of hydatid cyst on an in vitro model.Protoscoleces were aseptically aspirated from the naturally infected livers of sheep and goats. Various concentrations of AmB (2.5-20 mg/ml, Ag-NPs (0.5-4 mg/ml, F. vulgare essential oil (0.125-1 mg/ml and hypertonic saline (10-20% were used for 5-60 min. Eosin exclusion test was used to determine the viability of protoscoleces.Maximum protoscolicidal effect of AmB and Ag-NPs was found at concentrations of 20 and 4 mg/mL, resulting in only 82.3% and 71.6% of the protoscoleces after 60 min of incubation, respectively. In contrast, F. vulgare essential oil at concentration of 1 mg/ml and hypertonic saline 20% killed 100% protoscoleces after 5 and 10 min of exposure, respectively.The results indicated weak scolicidal activity of AmB and Ag-NPs; whereas F. vulgare essential oil had potent scolicidal activity against protoscoleces of hydatid cyst that revealed the potential of F. vulgare as a natural source for the production of new scolicidal agent for use in hydatid cyst surgery. However, further studies will be needed to confirm these results by checking the essential oil and its active component in the in vivo model.

  12. Foeniculum vulgare Mill. increases cytosolic Ca(2+) concentration and inhibits store-operated Ca(2+) entry in vascular endothelial cells.

    Science.gov (United States)

    Han, A Young; Lee, Hui Su; Seol, Geun Hee

    2016-12-01

    This study assessed the effects of essential oil of Foeniculum vulgare Mill. (fennel oil) and of trans-anethole, the main component of fennel oil, on extracellular Ca(2+)-induced store-operated Ca(2+) entry (SOCE) into vascular endothelial (EA) cells and their mechanisms of action. Components of fennel oil were analyzed by gas chromatography-mass spectrometry. Cytosolic Ca(2+) concentration ([Ca(2+)]c) in EA cells was determined using Fura-2 fluorescence. In the presence of extracellular Ca(2+), fennel oil significantly increased [Ca(2+)]c in EA cells; this increase was significantly inhibited by the Ca(2+) channel blockers La(3+) and nifedipine. In contrast, fennel oil induced [Ca(2+)]c was significantly lower in Ca(2+)-free solution, suggesting that fennel oil increases [Ca(2+)]c mainly by enhancing Ca(2+) influx into EA cells. [Ca(2+)]c mobilization by trans-anethole was similar to that of fennel oil. Moreover, SOCE was suppressed by fennel oil and trans-anethole. SOCE was also attenuated by lanthanum (La(3+)), a non-selective cation channel (NSC) blocker; 2-aminoethoxydiphenyl borane (2-APB), an inositol 1,4,5-triphosphate (IP3) receptor inhibitor and SOCE blocker; and U73122, an inhibitor of phospholipase C (PLC). Further, SOCE was more strongly inhibited by La(3+) plus fennel oil or trans-anethole than by La(3+) alone. These findings suggest that fennel oil and trans-anethole significantly inhibit SOCE-induced [Ca(2+)]c increase in vascular endothelial cells and that these reactions may be mediated by NSC, IP3-dependent Ca(2+) mobilization, and PLC activation.

  13. Efeitos de diferentes substratos e temperaturas na germinação de sementes de Foeniculum vulgare

    Directory of Open Access Journals (Sweden)

    R.E. RANZANI

    2016-01-01

    Full Text Available RESUMO O funcho (Foeniculum vulgare é uma espécie medicinal nativa da Europa e amplamente cultivada em todo o Brasil. Possui propriedades carminativas, digestivas e diuréticas. Dada a grande importância dessa espécie no âmbito medicinal, objetivou-se com este trabalho avaliar o efeito de diferentes substratos e temperaturas na germinação de sementes de funcho. Os tratamentos foram compostos de duas temperaturas sendo uma constante de 20ºC e outra alternada de 25-30 °C e três diferentes substratos: papel mata borrão (Germitest, areia e vermiculita, em esquema fatorial 3 x 2, com 4 repetições de 25 sementes. Foi avaliado o índice de velocidade de germinação (IVG, porcentagem de germinação (PG, comprimento da radícula (CR, massa fresca (MF e seca (MS de plântulas. A temperatura de 20 ºC proporcionou maiores IVG, porcentagens de germinação, comprimento de radícula e massa seca. A germinação de sementes de funcho não foi influenciada pelos diferentes substratos testados. Pelos resultados, conclui-se que a condição que permitiu maior porcentagem de germinação das sementes de funcho foi a temperatura de 20 °C atingindo 91% de germinação.

  14. The Effect of Hydro-Alcoholic Extract of Foeniculum vulgare Mill on Leukocytes and Hematological Tests in Male Rats

    Science.gov (United States)

    Mansouri, Esrafil; Kooti, Wesam; Bazvand, Maryam; Ghasemi Boroon, Maryam; Amirzargar, Ashraf; Afrisham, Reza; Afzalzadeh, Mohammad Reza; Ashtary-Larky, Damoon; Jalali, Nasrin

    2015-01-01

    Background: Medicinal plants have a long history in treating blood disorders, which is one of the most common problems in today's advanced world. Fennel (Foeniculum vulgare) is a medicinal plant with a high content of polyphenols and has antioxidant and immunomodulatory properties. Objectives: The aim of this study was to evaluate the effect of hydro-alcoholic extract of fennel on some hematological indices in male rats. Materials and Methods: In this experimental study, thirty male Wistar rats were divided into six groups (five rats in each group). The first group (control) did not receive any dose; the second group (sham) received 1 mL normal saline (extraction solvent); and the experimental groups 1, 2, 3 and 4 respectively received 1 mL hydro alcoholic extract of fennel in four doses of 250, 500, 750 and 1000 mg/kg of body weight every 48 hours for 30 days by gavage. One day after the last gavage following induction of anesthesia and taking blood from the heart of rats, measurement of red and white blood cells, hemoglobin, hematocrit and tests of bleeding and coagulation time (CT) were performed. The data was analyzed by one-way ANOVA test using SPSS15 software. Results: Fennel increased mean RBC (7.54 ± 0.53 × 106) and WBC (5.89 ± 0.78 × 103) values, especially at a dose of 250 mg/mL and CT (2.45 ± 0.20) at a dose of 500mg/mL compared to the control group (P < 0.05). Conclusions: Fennel increased red and white blood cells probably due to the presence of polyphenols and antioxidant activity of fennel and reduced negative effects of free radicals on blood cells. PMID:25866717

  15. Effect of Cymbopogon martinii, Foeniculum vulgare, and Trachyspermum ammi Essential Oils on the Growth and Mycotoxins Production by Aspergillus Species

    Directory of Open Access Journals (Sweden)

    Negero Gemeda

    2014-01-01

    Full Text Available This study was performed to investigate effect of essential oils on Aspergillus spore germination, growth, and mycotoxin production. In vitro antifungal and antiaflatoxigenic activities of Cymbopogon martinii, Foeniculum vulgare, and Trachyspermum ammi essential oils were carried out on toxigenic strains of Aspergillus species. Plant materials were hydrodistilled for 4-5 h in Clevenger apparatus. 0.25 μL/mL, 0.5 μL/mL, 1 μL/mL, 2 μL/mL, and 4 μL/mL concentrations of each essential oil were prepared in 0.1% Tween 80 (V/V. T. ammi oil showed highest antifungal activity. Absolute mycelial inhibition was recorded at 1 μL/mL by essential oils of T. ammi. The oil also showed complete inhibition of spore germination at a concentration of 2 μL/mL. In addition, T. ammi oil showed significant antiaflatoxigenic potency by totally inhibiting toxin production from A. niger and A. flavus at 0.5 and 0.75 μL/mL, respectively. C. martinii, F. vulgare, and T. ammi oils as antifungals were found superior over synthetic preservative. Moreover, a concentration of 5336.297 μL/kg body weight was recorded for LC50 on mice indicating the low mammalian toxicity. In conclusion, the essential oils from T. ammi can be a potential source of safe natural food preservative for food commodities contamination by Aspergillus species.

  16. Chemical constituents and toxicity of Agastache foeniculum (Pursh kuntze essential oil against two stored-product insect pests Componentes químicos y toxicidad del aceite esencial de Agastachefoeniculum (Pursh Kuntze contra dos plagas de insectos de productos almacenados

    Directory of Open Access Journals (Sweden)

    Asgar Ebadollahi

    2011-06-01

    Full Text Available The uncontrolled use of synthetic insecticides is a great hazard for the environment and consumers. Essential oils were introduced as low toxic agents against mammals and non-targeted insects. In this study, essential oil from aerial parts of blue giant hyssop (Agastache foeniculum [Pursh] Kuntze (Lamiaceae was isolated by the water steam distillation method with a Clevenger apparatus, and its chemical composition was studied by gas chromatography mass spectrometry. The toxicity of A. foeniculum essential oil against red flour beetle, Tribolium castaneum (Herbst, and lesser grain borer Rhyzopertha dominica (F. was evaluated by fumigation at 24, 48, and 72 h exposure times. Estragole and 1,8-cineole were identified as major constituents of the A. foeniculum oil. Fumigation bioassays revealed that A.foeniculum oil had strong insecticidal activity on experimental insects. Rhyzopertha dominica was more susceptible than T. castaneum for all exposure times. Insecticidal activity varied with essential oil concentration and exposure time. Probit analysis showed that increased exposure time and essential oil concentration increased mortality. These results indicated that A. foeniculum essential oil can be applied in the management of stored-product insects to decrease the detrimental effects of synthetic insecticides.El uso incontrolado de los insecticidas sintéticos causa gran peligro para el medio ambiente y los consumidores. Los aceites esenciales se presentan como agentes tóxicos leves contra mamíferos e insectos no objetivo. En el presente estudio, el aceite esencial de las partes aéreas del hisopo gigante azul (Agastache Foeniculum [Pursh] Kuntze (Lamiaceae se aisló por el método de destilación al vapor de agua, utilizando un aparato de Clevenger y se estudió su composición química mediante cromatografía de gases y espectrometría de masas. La toxicidad del aceite esencial de A. foeniculum se evaluó por métodos de fumigación a las 24

  17. Estudio de las asociaciones áfidos-entomófagos sobre Foeniculum vulgare (Umbelliferae) y Conyza bonariensis (Asteraceae) en la región central de Santa Fe, Argentina Study of the associations of aphids-entomophagous in Foeniculum vulgare (Umbelliferae) and Conyza bonariensis (Asteraceae) in the central region of Santa Fe, Argentina

    OpenAIRE

    Romina G. MANFRINO; Salto, César E.; Leticia Zumoffen

    2011-01-01

    El objetivo del presente trabajo fue estudiar las asociaciones áfidos-entomófagos en Foeniculum vulgare (Miller) y Conyza bonariensis L, a fin de implementar el Control Biológico Conservativo (CBC) en agroecosistemas de la región central de la provincia de Santa Fe. Se realizaron estudios, a campo, de la entomofauna presente mediante la observación directa de las plantas; las mismas se llevaron a cabo con una frecuencia semanal. En invernáculo, se procedió a la identificación de las especies ...

  18. Increase in Bcl2 expression of penile and prostate cells of Sprague Dawley male rats following treatment with buceng (combination of Pimpinella alpina molk with Eurycoma longifolia Jack

    Directory of Open Access Journals (Sweden)

    Taufiqurrachman Nasihun

    2015-04-01

    Full Text Available Background: Treatment with buceng combination of Eurycoma longifolia Jack and Pimpinella alpine Molk has been proven to increase testosterone level, decrease apoptosis and caspase3 expression. Bcl2 is an antiapoptotic protein found in cytoplasm which inhibits cells apoptosis. This study was aimed to investigate the effect of buceng on Bcl2 expression on penile and prostate tissues of the rats. Methods: In this experimental study, 24 male Sprague Dawley rats of 90 days old, weighing ± 300 grams, were randomly assigned into four groups. Group A, normal rats. Group B, castrated rats and treated with buceng 100 mg/day, per oral (Cast-Bcg; Group C, castrated rats and treated with 2 ml of water as placebo against buceng (Cast-Plac. Group D, castrated rats, treated with mesterolone 6.75 mg/day, per oral, as exogenous testosterone (Cast-Mest. All rats were treated for 30 days. Manova test was used to analyze the different expression of Bcl2 among groups with significance level at p ≤ 0.05. Results: Castration was associated with significant decrease of Bcl2 expression in the penile and prostate tissues (53.0 and 50.9%, respectively compared to normal rats (82.6 and 84.2%, respectively, p < 0.001. Treatment with mesterolone reversed Bcl2 expression (77.1 and 78.1% to a near normal level. The same level of Bcl2 expression was also observed with buceng treatment (73.8 and 78.2%.Conclusion: The treatment with buceng could enhance Bcl2 expression in penile and prostate tissues, comparable to normal rats and mesterolone treated rats.

  19. Comparative chemical composition and antioxidant activities of wild and cultivated Laurus nobilis L. leaves and Foeniculum vulgare subsp. piperitum (Ucria) coutinho seeds.

    Science.gov (United States)

    Conforti, Filomena; Statti, Giancarlo; Uzunov, Dimitar; Menichini, Francesco

    2006-10-01

    The chemical composition and antioxidant activities of wild and cultivated Laurus nobilis leaves and Foeniculum vulgare subsp. piperitum seeds were determined. Differences were found in the total phenolic content of fennel. GC-MS analysis of the non polar fractions showed a different composition between wild and cultivated plants. Cultivated laurel had a high content of terpenes such as linool, alpha-terpinol, alpha-terpinyl acetate, thymol, caryophyllene, aromandrene, selinene, farnesene, and cadinene, while wild laurel had a high content of eugenol and methyl eugenol, vitamin E, and sterols. The antioxidant potential of the extracts was determined using three complementary methods. Wild plants showed greater radical scavenging activity than the cultivated plants. The extracts also exhibited a significant antioxidant capacity also in the beta-carotene-linoleic acid test system. A high level of antioxidant activity was observed in wild laurel (IC50 = 1 microg/ml). Significant antioxidant activity measured in bovine brain was observed in wild laurel.

  20. Effects of Piriformospora indica and Sebacina vermifera on growth and yield of essential oil in fennel (Foeniculum vulgare) under greenhouse conditions.

    Science.gov (United States)

    Dolatabadi, Hossein Kari; Goltapeh, Ebrahim Mohammadi; Jaimand, Kamkar; Rohani, Neda; Varma, Ajit

    2011-02-01

    Fennel (Foeniculum vulgare) is a very important plant in the family of Apiaceae. Effects of inoculation of two endophytic fungi (Piriformospora indica and Sebacina vermifera) in growth, yield and composition of the essential oil of fennel (F. vulgare) were evaluated in pot cultures. Dry fruits were ground with an electric grinder and oil was extracted by hydrodistillation, and their composition was determined by GC/MS. In pot experiment, the maximum dry weight of the green tissue and root and plant height were obtained with P. indica, and maximum number of umbels per plant and dry weight of 1000 fruits were produced with S. vermifera. The P. indica and S. vermifera inoculation significantly increased oil yield as compared to non-inoculated control plants. GC and GC/MS studies revealed that the level of anethole was increased with P. indica and S. vermifera.

  1. Sublethal Effects of Essential Oils From Eucalyptus staigeriana (Myrtales: Myrtaceae), Ocimum gratissimum (Lamiales: Laminaceae), and Foeniculum vulgare (Apiales: Apiaceae) on the Biology of Spodoptera frugiperda (Lepidoptera: Noctuidae).

    Science.gov (United States)

    Cruz, G S; Wanderley-Teixeira, V; Oliveira, J V; Lopes, F S C; Barbosa, D R S; Breda, M O; Dutra, K A; Guedes, C A; Navarro, D M A F; Teixeira, A A C

    2016-04-01

    Spodoptera frugiperda (Smith 1797) (Lepidoptera: Noctuidae) is a major pest of maize, Zea mays L. Its control is often achieved through repeated applications per season of insecticides, which may lead to adverse effects on the ecosystem. Thus, the study of alternative methods with less environmental impact has expanded to include the use of essential oils. These oils are products of the secondary metabolism in plants, and their insecticidal activity has been widely demonstrated in populations of many pest insects. This study evaluated the insecticidal activities of essential oils from Eucalyptus staigeriana, Ocimum gratissimum, and Foeniculum vulgare on Spodoptera frugiperda. Gas chromatography–mass spectrometry profiles and contact toxicity of these oils as well as their sublethal effects on larvae and reproductive parameters in adults were evaluated. All three oils had sublethal effects on S. frugiperda; however, the oil of O. gratissimum showed the best results at all doses tested. These essential oils may have promise for control of S. frugiperda.

  2. 药用植物茴香的细胞学研究%Cytological Study on the Medical Plant Foeniculum vulgare Miller

    Institute of Scientific and Technical Information of China (English)

    邓瑞宁; 刘冰冰; 蔡明历; 郝大翠; 李润芳; 刘焰

    2006-01-01

    采用压片法,对生长在武汉地区的药用植物茴香(Foeniculum vulgare Miller)进行细胞学研究.结果表明:茴香的绝大多数花粉母细胞减数分裂过程中染色体的行为正常,终变期形成11个二价体,为染色体计数的最佳时期;茴香的染色体数目为2n=2x=22,核型公式为K(2n)=22=20m+2sm,其染色体相对长度组成为2n=2s+8M1+10M2+2L,属于"1A"对称型核型.

  3. A biogenic approach for green synthesis of silver nanoparticles using extract of Foeniculum vulgare and its activity against Staphylococcus aureus and Escherichia coli

    Directory of Open Access Journals (Sweden)

    SHITAL BONDE

    2011-07-01

    Full Text Available Bonde S. 2011. A biogenic approach for green synthesis of silver nanoparticles using extract of Foeniculum vulgare and its activity against Staphylococcus aureus and Escherichia coli. Nusantara Bioscience 3: 59-63. We report green synthesis of silver nanoparticles from extract of Foeniculum vulgare (fennel, saunf. The synthesis of silver nanoparticles was detected by changing color from green to brown after treatment with AgNO3 (1mM and the UV-visible spectrophotometer analysis showed the absorbance peak at about 427 nm, which indicates the synthesis of silver nanoparticles. Nanoparticle Tracking and Analysis (NTA by LM-20 was used for multi-parameter analysis, allowing for characterization of particle size and particle distribution of silver nanoparticles synthesized from extract of F. vulgare. NTA revealed the polydispersed nanoparticles in the range of 18-83 nm. Phytosynthesized silver nanoparticles showed antibacterial activity against the Staphylococcus aureus (ATCC-25923 and Escherichia coli (ATCC-39403. The silver nanoparticles also demonstrated remarkable antibacterial activity against two human pathogenic bacteria when used in combination with commercially available antibiotics. The bactericidal activity of the standard antibiotics was significantly enhanced in presence of silver nanoparticles against pathogenic bacteria, viz. E. coli-JM-103 (ATCC-39403 and S. aureus (ATCC-25923. Silver nanoparticles in combination with vancomycin showed maximum activity against E. coli (increase in fold area 5.76. and followed by S. aureus (1.08 and Gentamicin showed the maximum activity S. aureus (2.6 while E. coli (0.96. The approach of phytosynthesized silver nanoparticles using F. vulgare appears to be cost efficient, eco-friendly and easy alternative to conventional methods of synthesis.

  4. The Effects of Drought Stress on Morphological Traits and Yield of Three Medicinal Plants ( Coriandrum sativum, Foeniculum vulgare and Anethum graveolens in Greenhouse Conditions.

    Directory of Open Access Journals (Sweden)

    S. R Amiri Deh Ahmadi

    2012-07-01

    Full Text Available Drought stress is one of the important problems in arid and semi_arid regions. drought stress take place in plants when available water is lesser than evapotranspiration. In order to evaluate the effect of drought stress on morphological traits and yield of three medicinal plants an experiment was carried out during the 2010 year in research greenhouse of faculity of Agriculture, Ferdowsi University of Mashhad, in a factoriel arrengement based on a Completely Randomized Block Design with four replications. factors include 4 drought stress levels: Field capacity (100%, 75%, 50% and FC25% and 3 medicinal plants include: Coriandrum sativum, Foeniculum vulgare and Aniethum graveolens. The traits such as plant height, leaf number per plant, number of lateral branches per plant, umbrellas per plant, umbels per umbrellas, seed number per plant, seed weight per plant, 1000 seeds weight and harvest index were measured. Results showed that decreasing content of water in soil lesser than field capacity had significant effects on all of these traits. Coriandrum sativum in FC25% had maximum seed number in umbrellas(14 number, seed number per plant(27 number, Umbrellas per plant(4 number, umbels per umbrellas(10 number and 1000 seeds weight (6.5 gr. also Foeniculum vulgare in FC25% had minimum seed number in umbrellas(2 number, seed number per plant(2 number. Umbrellas per plant(0.25number, umbels per umbrellas(0.5 number and 1000 seeds weight (0.15 gr. Harvest index positive correlation with seed weight per plant and 1000 seeds weight.

  5. Caracterização da produtividade do funcho (Foeniculum vulgare Mill. no sertão de Sergipe Characterization of the of fennel's (Foeniculum vulgare Mill. yield semi-arid areas of Sergipe state

    Directory of Open Access Journals (Sweden)

    L.M. Carvalho

    2011-01-01

    Full Text Available Com o objetivo de avaliar a produtividade e qualidade do funcho (Foeniculum vulgare Mill. produzido nas condições edafoclimáticas de Sergipe, foi conduzido cultivo orgânico de funcho em Frei Paulo-SE. Em junho de 2008 e de 2009, início do período de chuvas, foram instalados plantios por meio de mudas. Adicionalmente, em dezembro de 2010 foi realizada colheita de frutos a partir de plantas obtidas após poda drástica e rebrota. Foi realizada colheita de frutos e determinação da produtividade de dezembro a janeiro de 2008, 2009 e em dezembro de 2010. Verificou-se produtividade de 720 Kg.ha-1, no primeiro plantio e de 349 Kg.ha-1 no plantio de 2009. As plantas obtidas por rebrota em 2010 resultaram em produtividade de 589 Kg.ha-1. Sugere-se que a maior produtividade verificada no primeiro ano seja decorrente da maior precipitação média e conseqüente maior disponibilidade de água. A produtividade verificada nas plantas que rebrotaram em 2010 foi também superior a verificada em 2009, o que pode ser explicado tanto em função da maior precipitação, quanto em função do provável maior desenvolvimento das raízes e maior número de ramos após poda, devido à quebra da dominância apical. Esses dados sugerem importância da disponibilidade hídrica na determinação da produtividade na região e explicam a variação na produtividade verificada. A massa seca dos frutos (5, 29 g para 1000 frutos e o alto teor de óleo essencial verificado (3,2%, assim como a composição do óleo, revelam a alta qualidade dos frutos produzidos na região.Aiming to evaluate the productivity and quality of fennel fruits produced in the countryside of Sergipe, with no irrigation, they were evaluated organic crops in Frei Paulo-SE. They were installed crops in 2008 and 2009 from seeds, on beginning rainy time. Seedlings obtained from seeds were transplanted in June 2008 and 2009. Besides, after the last harvest of the plants, grown in 2009, it was done a

  6. Contribution à l'étude de l'activité antimicrobienne des lipides totaux et des composé phénoloique des graines de foeniculum vulgar mill "besbes" de la région de Tlemcen

    OpenAIRE

    hamedi, sara née guaour

    2015-01-01

    Le rendement en poly phénol et en huile fixe ainsi que i'activite antimicrobienie ont été .étudiés sur les graines de fenouil (Foeniculum vulgar miii) « Besbes ». La teneur en eau a diéj vaIéur de 10% et l'huile fixe extraite par soxhiet a mentionné un rendement de 10.8%. Dans cette étude, les principes actifs de Foeniculum vuigar mill sont extraits par la méthode des solvants successifs: éthanol, eau et éther diétylique.. La détermination du rendement en phénols totaux a donné un...

  7. Immune effects of dietary anathole on Eimeria acervulina infection

    Science.gov (United States)

    Anethole is an aromatic, unsaturated ether that occurs as a major component of the essential oils of anise (Pimpinella anisum) and has shown antimicrobial properties in mammals. These broad spectrum pharmacologic activities suggest that anethole may also protect avian hosts against infectious diseas...

  8. Repellent activity of five essential oils against Culex pipiens.

    Science.gov (United States)

    Erler, F; Ulug, I; Yalcinkaya, B

    2006-12-01

    Essential oils extracted from the seeds of anise (Pimpinella anisum), dried fruits of eucalyptus (Eucalyptus camaldulensis), dried foliage of mint (Mentha piperita) and basil (Ocimum basilicum) and fresh foliage of laurel (Laurus nobilis) were tested for their repellency against the adult females of Culex pipiens. All essential oils showed repellency in varying degrees, eucalyptus, basil and anise being the most active.

  9. Molecular identification of Foeniculum vulgare and its adulterants by ITS2 sequence%ITS2序列鉴定小茴香及其常见混伪品

    Institute of Scientific and Technical Information of China (English)

    刘义梅; 罗焜; 陈科力; 陈士林

    2011-01-01

    目的 探讨基于ITS2序列鉴定小茴香及其混伪品的新方法.方法 对小茴香4份样品的ITS2序列进行PCR扩增和测序,同时从GenBank上下载小茴香及其常见混伪品共6个物种42个样本.用MEGA4.1计算其种间、种内的K-2-P距离,并分析各物种间ITS2序列二级结构的差异,最后利用ITS2序列构建其系统发育树.结果 小茴香种内最大K-2-P距离为0.0307,与混伪品的种间最小K-2-P距离为0.0405;小茴香及其混伪品的ITS2二级结构存在明显差异;构建的系统发育树显示小茴香的不同来源样本聚在一支,能很好与混伪品区分.结论 ITS2序列能够成功鉴定小茴香及其易混伪品,可以作为小茴香及其混伪品的分子鉴定方法.%Objective To explore the new method of discriminating Foeniculum vulgare from its adulterants by using ITS2 sequence. Method DNA samples were extracted from Foeniculum vulgare.ITS2 sequence was amplified by PCR and sequenced hidirectionally. Sequences were assembled hy CodonCode Aligner. The intra-specific and inter-specific K-2P distances of Foeniculum vulgare ancl its adulterants were calculated. and NJ tree was constructed by MEGA V 4. 1. ITS2 and secondary structure were predicted by the web tool established by Schultz etc. Results The maximum intra-specific K-2P distance was0. 0307 . while the minimum inter-specific K-2P distance was 0. 0405. Different samples of Foeniculum vulgare were gathered together and could be distinguished from its adulterants by NJ tree. And Foeniculum vulgare could he differentiated obviously from its adulterants by their ITS2 secondary structure. Conclusion Our study shows that ITS2 sequence is able to identify Foeniculum vulgare from its adulterants successfully and is an efficient molecular identification method for Foeniculum vulgare and its adulterants.

  10. 浙江产异叶茴芹叶挥发油化学成分研究%Study on the Chemical Components of Essentiale Oil from Zhejiang Pimpinella Diversifolia

    Institute of Scientific and Technical Information of China (English)

    徐晓卫; 林观样; 林崇良

    2012-01-01

    Objective To study chemical components in essentiale oil of Pimpinella diversifolia. Methods Essentiale oil was extracted by steam distillation. The components were separated and identified by GC - MS and elucidated on the standard MS spectral data. The relative contents in percentage were calculated by area normalization method. Results Thirty - eight principal components, about 94% of the oil, were identified. Main components were 1H - benzocycloheptene(22. 70% ), sesquiphellandrene(17. 77% ) and β - chamigrene(15. 94% ) . Conclusions Pimpinella diversifolia Ds. can be used as raw material of elemene and farnesene to extract phloridzin.%目的 研究浙江产伞形科茴芹属植物异叶茴芹叶挥发油的化学成分.方法 采用水蒸气蒸馏法提取挥发油,用气相色谱一质谱联用仪作定性分析,按峰面积归一化法计算挥发油中化学成分的相对百分含量.结果 鉴定出38个化合物,占总挥发油相对含量的94%.主要成分为IH-苯并环庚烯(22.70%)、水芹烯(17.77%)、β-花柏烯(15.94%),还含有一定量的β-榄香烯(2.99%)和β-法尼烯(1.75%).结论 浙江产异叶茴芹叶可作为提取榄香烯和法尼烯的原料药材.

  11. An experimental study of foeniculum oil-cyclodextrin inclusion complexes%小茴香挥发油环糊精包结物的研制

    Institute of Scientific and Technical Information of China (English)

    张延方

    2001-01-01

    OBJECTIVE To elucidate the inclusion effect of foeniculum oil andβ-Cyciodextrin(β-CD) in different proportion, and heat stability.METHODS Using grinding method to determine foeniculum oil-cyclodextrin inclusion complexes with diffrernt proportion, and then to determion recovery of the complexes.The stability wsa tested at 50 ℃ in an oven.RESULTS Recovery of inclusion complexes of five different proportion(oil:β-CD:1∶10,1:12.5,1∶15,1∶18,1:20 ml·g-1)were 73.56%, 85.83%,91.20%, 92.18% 92.85%,respetively.CONCLUSIONS The best proportion of the oil andβ-CD is 1∶15.%目的:探讨小茴香挥发油与不同比例β-环糊精(β-CD)包结的效果和规律,以及包结物的热稳定性。方法:用研磨法制成包结物,用常规方法测定包结物回收率,以电热干燥箱加热至50℃,测定其热稳定性。结果:小茴香挥发油与β-CD5种不同比例(1∶10,1∶12.5,1∶15,1∶18,1∶20)包结物的回收率分别为73.56%,85.83%,91.20%,92.18%,92.85。含1.25ml挥发油的包结物经70℃168h干燥后回收其挥发油的体积为1.02ml。结论:用研磨法进行包结时小茴香挥发油与β-CD之比(ml·g-1)以1∶15为宜,并且包结物的热稳定性良好。

  12. Effect of Fennel (Foeniculum Vulgare Mill. Used as a Feed Additive on The Egg Quality of Laying Hens Under Heat Stress

    Directory of Open Access Journals (Sweden)

    H Gharaghani

    2015-06-01

    Full Text Available In this study, one hundred and twenty 40-wk-old White Leghorn laying hens were submitted to two different thermal conditions (24° C vs. 34° C and were fed three levels (0, 10, or and 20 g/kg of dietof fennel fruits (Foeniculum vulgare Mill. as a feed additive in. This study was carried out according to a factorial design consisting of two temperatures and three fennel levels with five 5 replicates each (n = 2 × 3 × 5. Performance, egg production, egg quality, and oxidative product levels (malondialdehyde, MDA, and carbonyl in the eggs were measured before and after heat exposure. The results showed that the tested temperatures did not affect egg production (p>0.05, but the production of eggs with broken shell and feed intake were affected by heat stress (p0.05, but increased yolk triglyceride levels. Hens that consumed fennel presented lower yolk cholesterol and triglyceride levels (p<0.05. In general, fennel fruit influenced egg yolk cholesterol and triglyceride contents, and because of its antioxidant properties, it may alleviate the adverse effects of heat stress on laying hens.

  13. Inhibition on human liver cytochrome P450 3A4 by constituents of fennel (Foeniculum vulgare): identification and characterization of a mechanism-based inactivator.

    Science.gov (United States)

    Subehan; Zaidi, Syed F H; Kadota, Shigetoshi; Tezuka, Yasuhiro

    2007-12-12

    Fennel, a seed of Foeniculum vulgare, is used as a culinary spice and traditional medicine. The methanolic extract of fennel showed a characteristic of mechanism-based inactivation on erythromycin N-demethylation mediated by human liver microsomal cytochrome P450 3A4 (CYP3A4). The present study was conducted to identify the fennel constituent having the inhibition. Thirteen compounds have been isolated from a methanol extract of fennel and tested for their inhibition on CYP3A4. Among them, 5-methoxypsoralen (5-MOP) showed the strongest inhibition with an IC50 value of 18.3 microM and a mixed type of inhibition. In addition, with the preincubation time of 20 min only 5-MOP showed preincubation time dependency; the IC50 value decreased from 18.3 microM with a preincubation time of 0 min to 4.6 microM with a preincubation time of 20 min. Further investigation on 5-MOP showed the characteristics of time-dependent inhibition, requirement of NADPH, lack of protecting effect of nucleophiles, and recovery of CYP3A4 activity by the competitive inhibitor. This result suggests that the inhibitory activity of CYP3A4 by 5-MOP was a mechanism-based inactivation. The kinetic parameter for mechanism-based inactivation was characterized by a KI value of 15.0 microM and a kinact value of 0.098 min(-1).

  14. The Effect of Drought Stress on the Essential Oil Content and Some of the Biochemical Characteristics of Anise Hyssop (Agastache foeniculum [Pursh] Kuntze

    Directory of Open Access Journals (Sweden)

    Sajedeh Saeedfar

    2015-06-01

    Full Text Available Plot trials were carried out in a research field in Tehran (Iran to determine the effect of drought stress on the essential oil content and some of the plant biochemical characteristics of Anise Hyssop (Agastache foeniculum [Pursh] Kuntze, which is a valuable medicinal plant. Drought stress was conducted at different levels including: well-water (100% FC, mild drought stress (85% FC, moderate drought stress (70% FC, severe drought stress (55% FC, 100% FC (vegetative stage 85% FC (reproductive stage, 100% FC (vegetative stage 70% FC (reproductive stage, and 85% FC (vegetative stage 100% FC (reproductive stage. The experiment was arranged as a RCBD with three replications. The output results showed that, water deficit stress significantly (P≤0.05 increased activities of antioxidant enzymes (Superoxide Dismutase, Catalase, Glutathione Peroxidase as well as Essential Oil yield and Abscisic Acid content. Lipid and protein oxidation (malondialdehyde and dityrosine contents also increased significantly under severe water deficit stress. According to the results, severe drought conduction (55% FC is the optimum level of soil moisture to plant Anise Hyssop under water deficit stress.

  15. Chemomodulatory action of Foeniculum vulgare (Fennel) on skin and forestomach papillomagenesis, enzymes associated with xenobiotic metabolism and antioxidant status in murine model system.

    Science.gov (United States)

    Singh, B; Kale, R K

    2008-12-01

    The chemopreventive effect of different doses of test diet of Foeniculum vulgare Mill (Fennel) seeds was examined on DMBA-induced skin and B(a)P-induced forestomach papillomagenesis in Swiss albino mice. To the best of our knowledge, this is the first report of Fennel seeds exhibiting a significant reduction in the skin and the forestomach tumor incidence and tumor multiplicity as compared to the control group. Further, biochemical assays showed a significant increase in the content/activities of phase I enzymes especially in the case of 6% test diet. A concomitant increase in the activities of the phase II enzymes were observed with all the doses of test diet under study. A significant enhancement in the activities of antioxidant enzymes were observed especially at 4% and 6% test diets of Fennel. Glyoxalase I activity and the content of reduced glutathione were significantly elevated. Expectedly, the levels of peroxidative damage along with lactate dehydrogenase activity, exhibited a significant reduction at all three doses of test diets. These findings were indicative of chemopreventive potential of Fennel against carcinogenesis.

  16. Volatile components and key odorants of fennel (Foeniculum vulgare Mill.) and thyme (Thymus vulgaris L.) oil extracts obtained by simultaneous distillation-extraction and supercritical fluid extraction.

    Science.gov (United States)

    Díaz-Maroto, M Consuelo; Díaz-Maroto Hidalgo, Ignacio Javier; Sánchez-Palomo, Eva; Pérez-Coello, M Soledad

    2005-06-29

    Volatile oil extracts of fennel seeds (Foeniculum vulgare Mill.) and thyme leaves (Thymus vulgaris L.) were obtained by simultaneous distillation-extraction (SDE) and supercritical fluid extraction (SFE) and analyzed by gas chromatography-mass spectrometry (GC-MS). In general, fennel oil extracted by SDE and SFE showed similar compositions, with trans-anethole, estragole, and fenchone as the main components. In contrast, thymol and p-cymene, the most abundant compounds in thyme leaves, showed big differences, with generally higher amounts of monoterpenes obtained by SDE. However, in this case, the differences between the extracts were higher. Key odorants of fennel seeds determined by gas chromatography-olfactometry (GC-O) showed similar patterns when applying SDE and SFE. trans-Anethole (anise, licorice), estragole (anise, licorice, sweet), fenchone (mint, camphor, warm), and 1-octen-3-ol (mushroom) were the most intense odor compounds detected in fennel extracts. Thymol and carvacrol, with oregano, thyme, and spicy notes, were identified as key compounds contributing to the aroma of thyme leaves.

  17. 小茴香根皮化学成分研究%On the chemical constituents from the root barks of Foeniculum vulgare Mill

    Institute of Scientific and Technical Information of China (English)

    林健博; 古丽娜·沙比尔

    2014-01-01

    目的 分析新疆小茴香(Foeniculum vulgare Mill)根皮中的化学成分.方法 采用硅胶柱层析法反复分离纯化技术,通过质谱分析、核磁共振等现代波谱技术对新疆小茴香根皮中的化学成分进行结构鉴定.结果 从新疆小茴香中分离得到6种化合物:7-羟基-6-甲氧基香豆素(Scopoletin,Ⅰ).5-羟甲基糠(5-Hydroxymethylfurfural,Ⅱ)、焦谷氨酸乙酯(5-oxo-2-pyrrolidinecarboxylic acid;(±)-form,ET ester,Ⅲ)、胡萝卜苷(Daucosterol,Ⅳ)、β-谷甾醇(β-Sitosterol,V)、蔗糖(Sucrose,Ⅵ).结论 化合物Ⅰ~Ⅲ为首次从茴香属植物中分离得到,为进一步研究新疆小茴香的化学成分提供理论依据.

  18. Determination of Linoleic Acid in Foeniculum vulgare Mill. from Different Habitats by HPLC-ELSD%HPLC-ELSD 法测定不同产地小茴香中亚油酸含量

    Institute of Scientific and Technical Information of China (English)

    钱生勇; 顾湘

    2014-01-01

    目的:建立小茴香中亚油酸含量的 HPLC-ELSD 测定方法并测定不同产地小茴香的亚油酸含量。方法:采用 Cosmosil 5 C18-MS-Ⅱ柱,甲醇-水-冰醋酸(95∶5∶0.1%),流速1.0 mL· min-1,柱温35℃,漂移管温度40℃,雾化气 N2,载气流速3.0 L·min-1。结果与结论:本法测定小茴香中亚油酸含量简便可靠。测得4个产地小茴香中亚油酸含量最高为8.73 mg·g-1,最低为1.70 mg·g-1。不同产地小茴香中亚油酸含量差异明显。%Objective: To establish a quantitative HPLC-ELSD method for the determination of linoleic acid in Foeniculum vulgare Mill. from different habitats. Methods: A column of Cosmosil 5C18-MS-Ⅱ was used with the mobile phase of methanol and water and acetic acid (95∶5∶0.1%), the fIow rate was 1.0 mL· min-1, and the column temperature was set at 35 ℃. The temperature of drift tube was set at 40 ℃ with an air fIow rate of 3.0 L·min-1 for ELSD. Results and Conclusion: The established HPLC-ELSD method is simple and suitable for the quality control of Foeniculum vulgare Mill. The maximum content of linoleic acid in Foeniculum vulgare Mill. from four habitats was 8.73 mg·g-1 and the minimum content was 1.70 mg· g-1. Great difference of linoleic acid content is shown in Foeniculum vulgare Mill. from different habitats.

  19. Assessment of Free Radical Scavenging Potential and Oxidative DNA Damage Preventive Activity of Trachyspermum ammi L. (Carom and Foeniculum vulgare Mill. (Fennel Seed Extracts

    Directory of Open Access Journals (Sweden)

    Nandini Goswami

    2014-01-01

    Full Text Available Oxidation of biomolecules such as carbohydrates, proteins, lipids, and nucleic acids results in generation of free radicals in an organism which is the major cause of onset of various degenerative diseases. Antioxidants scavenge these free radicals, thereby protecting the cell from damage. The present study was designed to examine the free radical scavenging potential and oxidative DNA damage preventive activity of traditionally used spices Trachyspermum ammi L. (carom and Foeniculum vulgare Mill. (fennel. The aqueous, methanolic, and acetonic extracts of T. ammi and F. vulgare seeds were prepared using soxhlet extraction assembly and subjected to qualitative and quantitative estimation of phytochemical constituents. Free radical scavenging potential was investigated using standard methods, namely, DPPH radical scavenging assay and ferric reducing antioxidant power assay along with the protection against oxidative DNA damage. The results stated that acetonic seed extracts (AAcSE and FAcSE of both the spices possessed comparatively high amount of total phenolics whereas methanolic seed extracts (AMSE and FMSE were found to have highest amount of total flavonoids. At 1 mg/mL concentration, highest DPPH radical scavenging activity was shown by FMSE (96.2%, AAcSE was recorded with highest FRAP value (2270.27 ± 0.005 μmol/L, and all the seed extracts have been shown to mitigate the damage induced by Fenton reaction on calf thymus DNA. Therefore, the study suggests that T. ammi and F. vulgare seed extracts could contribute as a highly significant bioresource of antioxidants to be used in our day-to-day life and in food and pharmaceutical industry.

  20. Assessment of free radical scavenging potential and oxidative DNA damage preventive activity of Trachyspermum ammi L. (carom) and Foeniculum vulgare Mill. (fennel) seed extracts.

    Science.gov (United States)

    Goswami, Nandini; Chatterjee, Sreemoyee

    2014-01-01

    Oxidation of biomolecules such as carbohydrates, proteins, lipids, and nucleic acids results in generation of free radicals in an organism which is the major cause of onset of various degenerative diseases. Antioxidants scavenge these free radicals, thereby protecting the cell from damage. The present study was designed to examine the free radical scavenging potential and oxidative DNA damage preventive activity of traditionally used spices Trachyspermum ammi L. (carom) and Foeniculum vulgare Mill. (fennel). The aqueous, methanolic, and acetonic extracts of T. ammi and F. vulgare seeds were prepared using soxhlet extraction assembly and subjected to qualitative and quantitative estimation of phytochemical constituents. Free radical scavenging potential was investigated using standard methods, namely, DPPH radical scavenging assay and ferric reducing antioxidant power assay along with the protection against oxidative DNA damage. The results stated that acetonic seed extracts (AAcSE and FAcSE) of both the spices possessed comparatively high amount of total phenolics whereas methanolic seed extracts (AMSE and FMSE) were found to have highest amount of total flavonoids. At 1 mg/mL concentration, highest DPPH radical scavenging activity was shown by FMSE (96.2%), AAcSE was recorded with highest FRAP value (2270.27 ± 0.005 μmol/L), and all the seed extracts have been shown to mitigate the damage induced by Fenton reaction on calf thymus DNA. Therefore, the study suggests that T. ammi and F. vulgare seed extracts could contribute as a highly significant bioresource of antioxidants to be used in our day-to-day life and in food and pharmaceutical industry.

  1. Foeniculum vulgare Mill. Protects against Lipopolysaccharide-induced Acute Lung Injury in Mice through ERK-dependent NF-κB Activation.

    Science.gov (United States)

    Lee, Hui Su; Kang, Purum; Kim, Ka Young; Seol, Geun Hee

    2015-03-01

    Foeniculum vulgare Mill. (fennel) is used to flavor food, in cosmetics, as an antioxidant, and to treat microbial, diabetic and common inflammation. No study to date, however, has assessed the anti-inflammatory effects of fennel in experimental models of inflammation. The aims of this study were to investigate the anti-inflammatory effects of fennel in model of lipopolysaccharide (LPS)-induced acute lung injury. Mice were randomly assigned to seven groups (n=7~10). In five groups, the mice were intraperitoneally injected with 1% Tween 80-saline (vehicle), fennel (125, 250, 500µl/kg), or dexamethasone (1 mg/kg), followed 1 h later by intratracheal instillation of LPS (1.5 mg/kg). In two groups, the mice were intraperitoneally injected with vehicle or fennel (250µl/kg), followed 1 h later by intratracheal instillation of sterile saline. Mice were sacrificed 4 h later, and bronchoalveolar lavage fluid (BALF) and lung tissues were obtained. Fennel significantly and dose-dependently reduced LDH activity and immune cell numbers in LPS treated mice. In addition fennel effectively suppressed the LPS-induced increases in the production of the inflammatory cytokines interleukin-6 and tumor necrosis factor-alpha, with 500µl/kg fennel showing maximal reduction. Fennel also significantly and dose-dependently reduced the activity of the proinflammatory mediator matrix metalloproteinase 9 and the immune modulator nitric oxide (NO). Assessments of the involvement of the MAPK signaling pathway showed that fennel significantly decreased the LPS-induced phosphorylation of ERK. Fennel effectively blocked the inflammatory processes induced by LPS, by regulating pro-inflammatory cytokine production, transcription factors, and NO.

  2. Estudio de las asociaciones áfidos-entomófagos sobre Foeniculum vulgare (Umbelliferae y Conyza bonariensis (Asteraceae en la región central de Santa Fe, Argentina Study of the associations of aphids-entomophagous in Foeniculum vulgare (Umbelliferae and Conyza bonariensis (Asteraceae in the central region of Santa Fe, Argentina

    Directory of Open Access Journals (Sweden)

    Romina G. Manfrino

    2011-06-01

    Full Text Available El objetivo del presente trabajo fue estudiar las asociaciones áfidos-entomófagos en Foeniculum vulgare (Miller y Conyza bonariensis L, a fin de implementar el Control Biológico Conservativo (CBC en agroecosistemas de la región central de la provincia de Santa Fe. Se realizaron estudios, a campo, de la entomofauna presente mediante la observación directa de las plantas; las mismas se llevaron a cabo con una frecuencia semanal. En invernáculo, se procedió a la identificación de las especies cuando esta no fue posible a campo. La especie de áfido encontrada fue Hyadaphis foeniculi (Passerini (Homoptera: Aphididae, colonizando a F. vulgare y se destacó Uroleucon (Lambersius sp. (Homoptera: Aphididae enC. bonariensis. Entre los enemigos naturales, se destacaron los parasitoides y depredadores en F. vulgare y C. bonariensis, respectivamente. Para el estudio de las relaciones áfidos - enemigos naturales, se calcularon los coeficientes de correlación; los valores indicaron una relación entre H. foeniculi y Diaretiella rapae (M'Intosh (Hymenoptera: Braconidae (0,63 y entre Uroleucon (Lambersius sp. y coccinélidos (Coleoptera: Coccinellidae (0,43. Las especies vegetales estudiadas son muy importantes para mantener los distintos grupos de entomófagos en los agroecosistemas y su utilización en programas de CBC de pulgones plaga es posible, contribuyendo así a reducir las aplicaciones de insecticidas en cultivos.The objective of this work was to study the aphids-entomophagous associations in Foeniculum vulgare (Miller and Conyza bonariensis L. in order to implement Conservative Biological Control (CBC in agroecosystems of the central region of Santa Fe Province. Direct observation on plants was conducted on a weekly basis, from February to May 2008. The identification of species was carried out in the greenhouse when it was not possible to do it in the field. The aphid species identified on F. vulgare was Hyadaphis foeniculi (Passerini

  3. Effects of foeniculum vulgare mill on lipid peroxidation in rats with liver hepatic fibrosis%小茴香对肝纤维化大鼠脂质过氧化水平的影响

    Institute of Scientific and Technical Information of China (English)

    范强; 阿地力江·伊明; 王水泉; 甘子明

    2011-01-01

    目的:研究小茴香对肝纤维化大鼠的脂质过氧化水平的影响.方法:取Wister大鼠100只,随机取12只设为正常组,其余采用四氯化碳(CCL4)复合因素法复制肝纤维化大鼠模型,复制成功后随机分为模型对照组、小茴香组和复方丹参组(n=12);相应药物干预8周后,镜检肝组织形态学改变;全自动生化分析仪检测外周血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平与血清总蛋白(TP)、白蛋白(ALB)含量;生化法检测肝组织羟脯氨酸(HYP)与血清丙二醛(MDA)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、过氧化氢酶(CAT)活性.结果:HE结果显示:模型组肝脏炎症与脂肪变性明显,经小茴香干预后明显改善.生化检测结果显示:与正常组比较,模型组ALT、AST水平显著升高(P<0.05),TP、ALB含量显著降低(P<0.05),HYP、MDA含量显著增加(P<0.05),而SOD、GSH-PX和CAT活性显著降低(P<0.05);与模型组比较,小茴香组ALT、AST水平显著下降(P<0.05),TP、ALB水平显著升高(P<0.05);HYP、MDA含量显著降低(P<0.05),SOD、GSH-PX和CAT活性显著提高(P<0.05).结论:小茴香的抗肝纤维化作用可能与调节大鼠脂质过氧化水平有关.%Objective: To observe the effects of foeniculum vulgare mill on liver fibrosis. Methods: 100 Wister rats were randomly divided into 4 groups as control group,model group, foeniculum vulgare mill group, and danshen group. Liver fibrosis models were established by the combination of injecting carbon tetrachloride (CCL4) subcutaneously. Drugs were lavaged for 8 weeks, rats livers tissue were examined with HE staining; the serum level of alanine transaminase (ALT), aspartate aminotransferase(AST), albumin(ALB) and total protern (TP) were determined using corresponding test kits; the hepatic lipid peroxidation indexes, including malondialdehyde (MDA), Superoxide dismutase(SOD),Glutathione peroxidase(GSH-PX),Catalase(CAT) were measured

  4. Approach to pharmacological and clinical applications of Anisi aetheroleum

    Directory of Open Access Journals (Sweden)

    Khaled Mohamed Mohamed Koriem

    2015-01-01

    Full Text Available Anisi aetheroleum is the oil obtained from Pimpinella anisum L. (P. anisum by steam distillation. P. anisum seeds were air-dried, and then the dry seeds were crushed, pulverized, and weighed in sequence for anise oil preparation. P. anisum is one of the oldest medicinal plants that belong to family Apiaceae. The fruit of P. anisum is harvested in August and September. P. anisum is widespread in Asia, Africa and Europe. Local names of P. anisum include anise, anisoon, roomy, saunf, sweet cumin and yansoon. The anise oil odour is aromatic while the oil tastes sweet. The average daily dose of Anisi aetheroleum is 0.3 g. trans-Anethole is the major ingredient of the anise oil. Anisi aetheroleum also displays a protective action against neurotoxicity. In addition, Anisi aetheroleum increases glucose absorption and reduces urine output in the rat. The plant oil have pharmacological (antimicrobial, hepatoprotective, anticonvulsant, anti-inflammatory, antispasmodic, bronchodilator, estrogenic, expectorant and insecticidal effects and clinical effects on nausea, constipation, menopausal period, virus, diabetes, obesity and sedative action. Owing to the wide application of Anisi aetheroleum in pharmacological and clinical fields, it is recommended for more clinical trails to discover a new medication from the active constituents of the plant oil in the future to treat human diseases especially chronic ones.

  5. Chemical compositions and antibacterial effects of essential oils of Turkish oregano (Origanum minutiflorum), bay laurel (Laurus nobilis), Spanish lavender (Lavandula stoechas L.), and fennel (Foeniculum vulgare) on common foodborne pathogens.

    Science.gov (United States)

    Dadalioglu, Itir; Evrendilek, Gulsun Akdemir

    2004-12-29

    Chemical compositions and inhibitory effects of essential oils of Turkish oregano (Origanum minutiflorum O. Schwarz & P. H. Davis), bay laurel (Laurus nobilis L.), Spanish lavender (Lavandula stoechas subsp. stoechas L.), and fennel (Foeniculum vulgare Mill.) on Escherichia coli O157:H7, Listeria monocytogenes, Salmonella typhimurium, and Staphylococcus aureus were determined. After the essential oils were applied on the foodborne pathogens at doses of 0 (control), 5, 10, 20, 30, 40, 50, and 80 microL/mL, the resultant numbers of cells surviving were counted. Results revealed that all essential oils exhibited a very strong antibacterial activity against the tested bacteria (P < 0.05). Gas chromatography-mass spectrophotometry analyses revealed that carvacrol (68.23%), 1,8-cineole (60.72%), fenchone (55.79%), and trans-anethole (85.63%) were the predominant constituents in Turkish oregano, bay laurel, Spanish lavender, and fennel essential oils, respectively.

  6. COMPARATIVE STUDIES ON FOENICULUM MILLER IN CHINA AND THE TAXONOMIC TREATMENT%中国茴香属的比较研究及分类处理意见

    Institute of Scientific and Technical Information of China (English)

    范昌发; 何兴金; 贾敬芬; 李存勇; 冯陪章

    2001-01-01

    从细胞学、孢粉学、形态学角度,并辅以解剖学和胚胎学方法,对茴香属Foeniculum Miller植物球茎茴香F. vulgare subsp. copillaceum var.azoricum (Miller) Thellung与茴香F. vulgare Miller进行了比较研究.结果表明,孢粉学特征差异表现在花粉粒类型、形状、大小、萌发孔、纹饰等方面;细胞学特征差异表现在核型不对称系数、染色体组成、最长染色体与最短染色体之比值等方面;形态学特征差异包括基部茎、基部叶叶鞘及果实主棱的圆钝等方面;解剖学上发现数量性状差异;胚胎学方面则表现在珠被上.就上述对应特征而言,茴香均表现出比球茎茴香更为进化的特性.据此,支持Miller(1768)将球茎茴香视与茴香均作为种等级的处理意见,而不支持将球茎茴香视为茴香变种,以及为茴香异名的观点,并恢复其名称为Foeniculum azoricum Miller.

  7. Effect of Intercropping Patterns of Fennel (Foeniculum vulgarMill, Sesame (Sesamum indicum and Bean (Phaseolus vulgaris L on Growth, Qualitative and Quantitative Characters and Yield Components

    Directory of Open Access Journals (Sweden)

    fatemeh ranjbar

    2017-02-01

    Full Text Available Introduction: Ecological agriculture is an integrated system that gives credit to higher quality of products. Using of ecological agriculture and low input systems or other similar systems as a replacement for conventional systems turn out to progress in sustainable agriculture and protecting environment health. One of the best approaches to achieve these goals is to use mixed farming. Many experiments have shown that mixed farming has higher yield than sole cropping. The other benefits of mixed farming are: management of insects, weeds and diseases, promotion of diversity, improvement of products quality and also increase in stability and sustainability. These goals also achieved by decreasing in use of non-renewable resources and also reducing environment risks. Hence, assessment of intercropping patterns of Fennel (Foeniculum vulgar, Sesame (Sesamum indicum and Bean (Phaseolus vulgaris on qualitative and quantitative characters and yield components were the purposes of this experiment. Materials and methods: In order to study yield and yield components in different intercrops of fennel, sesame and bean, an experiment was conducted in Agricultural Research Station, Ferdowsi University of Mashhad during 2010-2011growing season. The experimental design was a Randomized Complete Block with three replications. The treatments were consisted of: pure stand of fennel, sesame and bean, row intercropping of sesame-bean with recommended density (1:1, fennel-bean (1:1, fennel-sesame (1:1 and intercrops of fennel- sesame - bean (1:1:1. The field of experiment was prepared at the end of March, a month before sowing; 30 ton/ hectare manure fertilizer was used. Because of sowing these 3 crops in a low input system, non-chemical approaches to control weeds and diseases during the growth season were employed. In order to determine crops seed yield and their biological yield in this experiment, sampling was done after omitting of margin effects (0.5 m first and

  8. Determination of chemopreventive role of Foeniculum vulgare and Salvia officinalis infusion on trichloroacetic acid-induced increased serum marker enzymes lipid peroxidation and antioxidative defense systems in rats.

    Science.gov (United States)

    Celik, Ismail; Isik, Ismail

    2008-01-10

    Today's world is increasingly seeking ways to replace the synthetic drugs with the therapeutic power of natural products. This study was designed to investigate the protective effects of Foeniculum vulgare (FV) and Salvia officinalis (SO) waters infusions against carcinogen chemical trichloroacetic acid (TCA)-exposure in rats. The chemopreventive potential of the plant infusions were evaluated by measuring levels of serum marker enzymes [aspartate aminotransferase (AST), alanin aminotransferase (ALT), creatine phosphokinase (CPK), acid phosphatase (ACP), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH)], antioxidant defense systems [Reduced glutathione (GSH), glutathione reductase (GR), superoxide dismutase (SOD), glutathione-S-transferase (GST) and catalase (CAT)] and lipid peroxidation level (Malondialdehyde = MDA) in various tissues of rats. Female Sprague-Dawley rats, weighing 150-200 g, were randomly allotted into four experimental groups. While the control group (A) received only natural spring water, the treatment B group (0.2% TCA) supplied with the drinking water containing 0.2% TCA, the treatment C (TCA + FV infusion) and D (TCA + SO infusion) groups drank the drinking water containing 0.2% TCA and 2.5% the plant grains and leaves ad libitum for 50 days during experiment. At the end of the 50 days experiment, TCA and the plant's infusions caused different affect on the serum marker enzymes, tissues antioxidant defense systems and lipid peroxidation against TCA-exposed in rats with comparison to those of TCA exposed and control rats. According to the results, both TCA and TCA + plants infusions caused a significant increase in serum AST, ALT and CPK activity. Non-enzymic antioxidant GSH level significantly increased in the brain whereas reduced in the erythrocytes and kidney of TCA + FV and TCA + SO as compared to TCA group and control. While MDA content slightly increased in tissues of TCA group in comparison to those of control, significantly

  9. The Changes of Volatile Components in Foeniculum vulgare Mill During Storage%小茴香储存过程中挥发性成分的变化

    Institute of Scientific and Technical Information of China (English)

    罗静; 钟永科; 李明明; 范会

    2016-01-01

    The changes of volatile components in Foeniculum vulgare Mill during 12 months'storage are analyzed and discussed using gas chromatography-mass spectrometry (GC-MS)coupled to solid phase microextraction (SPME).It is found that besides the main ingredients trans-anethole and estragole (the relative total content is 72.2%,85.49%,93.06%,95.89%,90.13% in the origin and 3,6,9,12 months'storage respectively),the content of others changes significantly.The relative content of anisealdehyde increases to 6.1% from 0.47% after the storage of 12 months.The relative content of linalool reduces to 0.28% from 9.72% at the time of 6 months'storage.With the same storage time,the relative total content of terpenoids reduces to 2.5 1% from 14.87% (the main ingredient limonene reduces to 1.52% from 7.98%).Besides,small amount of esters (at the medium of storage),acids and alcohols (at the end of storage)is also detected in the storage.%采用固相微萃取-气质联用技术,测定了储存12个月的小茴香挥发性成分,探讨了其储存中挥发性成分的相对含量变化。结果发现:小茴香储存中除主要成分反式茴香脑和爱草脑的含量相对稳定外(在储存初始及3,6,9,12个月的总含量分别是72.2%,85.49%,93.06%,95.89%,90.13%),其他成分含量变化较大,其中,茴香醛含量增加,由起初的0.47%不断增加到6.1%;芳樟醇和萜类成分含量减小,且都在储存前半期,芳樟醇由初期的9.72%减少到0.28%,而萜类成分个数由14个减少到10个,总含量也由14.87%减少到2.51%(主要成分柠檬烯由初期的7.98%减少到1.52%)。此外,小茴香在储存中期还有少量酯类产生,尾期主要是酸类和醇类,它们的含量均不高。

  10. 茴芹的周年生产技术要点

    Institute of Scientific and Technical Information of China (English)

    齐辉

    2004-01-01

    茴芹(Pimpinella anisum Nakai.)为伞形科、茴芹属1年生草本植物。原产于欧亚大陆,以希腊、埃及为多。种子可作为食品的调味料及药用;幼苗可作青菜或沙拉配菜用。

  11. 茴香提取液对地塞米松诱导大鼠胰岛素抵抗的影响%Foeniculum vulgare extract reduces dexamethasoneinduced insulin resistance in rats

    Institute of Scientific and Technical Information of China (English)

    王彩冰; 黄彦峰; 黄丽娟; 何显教; 晋玲; 赵善民

    2012-01-01

    AIM: To investigate the impact oifoeniculum vulgare extract on dexamethasone-induced insulin resistance (IR) in rats.METHODS: Fifty rats were randomly divided into five groups: normal control group, model group, metformin group, low- and high-dose foeniculutn vulgare extract groups. Except for the normal control group, the remaining groups were intramuscularly given dexamethasone (1 mg/kg) every other day. The normal control group and model group were intragastrically given saline solution, while the metformin group and low- and high-dose foeniculum vulgare extract groups were intragastrically given metformin (40 g/L) and 300 and 600 g/L oifoeniculumvulgare extract once a day, respectively. After 15 days of drug use, fasting blood glucose (FBG) and serum insulin (FINS) were determined, and glucose tolerance test (OGTT) was performed. The OGTT120' recovery percentage, insulin sensitivity index (ISI) and insulin resistance index (HOMA-IR) were calculated.RESULTS: The metformin group, low- and high-dose foeniculum vulgare groups and normal control group had comparable FBG and OGTT120' recovery percentage. FBG was significantly lower and the OGTT120' recovery percentage was significantly higher in the three treatment groups than in the model group (all P < 0.05 or 0.01). FINS in the three treatment groups was significantly higher than that in the normal control group but lower than that in the model group (all P < 0.05 or 0.01). ISI in the three treatment groups was significantly lower than that in the normal control group but higher than that in the model group (all P < 0.01). HOMA-IR in the three treatment groups was significantly lower than that in the model group (all P < 0.01). The low-dose foeniculum vulgare group had significantly lower ISI than the high-dose group (P < 0.05). As foeniculum vulgare dosage increased, FBG, OGTT120', FINS, ISI and HOMA-IR were closer to levels in the normal control group.CONCLUSION: Foeniculum vulgare can enhance the sensitivity of

  12. Antifungal activity of essential oils against selected terverticillate penicillia.

    Science.gov (United States)

    Felšöciová, Soňa; Kačániová, Miroslava; Horská, Elena; Vukovič, Nenad; Hleba, Lukáš; Petrová, Jana; Rovná, Katarina; Stričík, Michal; Hajduová, Zuzana

    2015-01-01

    The aim of this study was to screen 15 essential oils of selected plant species, viz. Lavandula angustifolia, Carum carvi, Pinus mungo var. pulmilio, Mentha piperita, Chamomilla recutita L., Pinus sylvestris, Satureia hortensis L., Origanum vulgare L., Pimpinella anisum, Rosmarinus officinalis L., Salvia officinalis L., Abietis albia etheroleum, Chamomilla recutita L. Rausch, Thymus vulgaris L., Origanum vulgare L. for antifungal activity against five Penicillium species: Penicillium brevicompactum, Penicillium citrinum, Penicillium crustosum, Penicillium expansum and Penicillium griseofulvum. The method used for screening included the disc diffusion method. The study points out the wide spectrum of antifungal activity of essential oils against Penicillium fungi. There were five essential oils of the 15 mentioned above which showed a hopeful antifungal activity: Pimpinella anisum, Chamomilla recutita L., Thymus vulgaris, Origanum vulgare L. The most hopeful antifungal activity and killing effect against all tested penicillia was found to be Origanum vulgare L. and Pimpinella anisum. The lowest level of antifungal activity was demonstrated by the oils Pinus mungo var. pulmilio, Salvia officinalis L., Abietis albia etheroleum, Chamomilla recutita L. Rausch, Rosmarinus officinalis.

  13. Chemical components of volatile oil in roots of Foeniculum vulgare mill from different habitats by GC-MS%不同产地茴香根皮挥发油成分的GC-MS分析

    Institute of Scientific and Technical Information of China (English)

    宋凤凤; 美丽万·阿不都热依木; 周静

    2014-01-01

    To analyze and compare the chemical components of the volatile oil in roots of Foeniculum vul-gare mill from ten different habitats. The volatile oil was extracted by steam distillation. The components of volatile oil were separated and analyzed by GC-MS. The relative content of each constituent was calculated by normalization. Result showed that twenty-six components in the volatile oil of roots of Foeniculum vul-gare mill from ten different habitats were identified. 13,7,11,10,14,11,14,11,10,10 components were identified from Xiaoerbage,Lawati,Tacheng,Bageqi,Shufu,Lasikui,Manglai,Yili,Moyu,Buzhake of Xin-jiang. Dill Apiol and 1,3-benzodioxole,4,7-dimethoxy-5-(2-propen-1-yl)-and 1,3-benzodioxole,4-me-thoxy-6-(2-propenyl)-are its common and main ingredients,which account for 90. 81% ~96. 18% and 0. 54% ~2. 76% and 0. 10% ~3. 33% of the total oils.%采用水蒸气蒸馏法提取10个不同产地小茴香根挥发油,运用气相色谱-质谱联用技术( GC-MS)进行化学成分分析,面积归一化法测定各成分的相对百分含量。结果表明,从10个不同产地的茴香根挥发油中共鉴定出26个成分,其中肖尔巴格乡、阿瓦提乡、塔城、巴格其镇、疏附县、拉丝奎镇、芒来乡、伊犁、墨玉县、布扎克乡分别鉴定出了13、7、11、10、14、11、14、11、10、10个成分,其中莳萝芹菜脑、洋芹脑和肉豆蔻醚是它们的共有成分和主要成分,莳萝芹菜脑占挥发油总量的90.81%~96.18%,洋芹脑占挥发油总量的0.54%~2.76%,肉豆蔻醚占挥发油总量的0.10%~3.33%。

  14. Study on preventive effect of volatile oil extracted from Foeniculum vulgare Mill. Seeds on postoperative intra-abdominal adhesions%小茴香挥发油对术后腹腔黏连的预防作用探讨

    Institute of Scientific and Technical Information of China (English)

    滕光寿; 王成果; 毛峰峰; 张琰; 刘兴友; 杨鹏; 石磊; 刘曼玲

    2011-01-01

    Objective To study the preventive effect of volatile oil extracted from Foeniculum vulgare Mill. Seeds on the formation of postoperative intra-abdominal adhesion. Methods Thirty-eihgt SD rats were randomly divided into sham operation group (10), operation group (14) and volatile oil treated group (14):sham operation group was only operated by abdominal incision, the rest two groups were established animal model of abdominal adhesion by rubbing the procussus vermiformis of cecum with dry sterile gauze, clamping and scuffing abdominal wall. Half of rats were separately killed on day 7 and day 14 after surgery, respectively.The degree of adhesion was evaluated according to Phillips 5-scale grade and the feature of this model. Results The scores of intra-abdominal adhesion were significantly lower in the carbachol group than those in operation group both on 7 d and 14 d(P<0.01 ). Conclusion Volatile oil extracted from Foeniculum vulgare Mill. Seeds may take a significant role in the prevention of postoperative abdominal adhesion in rats.%目的 探讨小茴香挥发油对术后腹腔黏连的预防作用.方法 将38只雄性SD大鼠按随机数字表法分为假手术组(10只)、手术对照组(14只)及小茴香挥发油组(14只).假手术组只开腹,后两组开腹后无菌干纱布摩擦大鼠盲肠蚓突部,以钳夹和刮伤腹壁法制作大鼠腹腔黏连动物模型.各组于术后第7 d、14 d各处死一半动物,参考Phillips 5级分类法并结合本模型特点进行大体黏连程度分级评分.结果 小茴香挥发油组第7 d、14 d大体黏连程度评分均明显低于手术对照组,差异有统计学意义(P<0.01).结论 小茴香挥发油可预防大鼠腹腔黏连形成.

  15. Effects of mycorrhiza inoculation and different irrigation levels on yield, yield components and essential oil contents of fennel (Foeniculum vulgare Mill. and ajwain (Trachyspermum ammi L.

    Directory of Open Access Journals (Sweden)

    A Koocheki

    2016-05-01

    Full Text Available Introduction Fertilizers are the key components which provide plant nutrients' needs in recent years (Omid Jangir & Sing, 1996; Kapoor et al., 2007. In many cases, using chemical fertilizers has different negative environmental effects such as soil, water and air pollution, which increase environmental hazardous and production costs (Jangir & Sing, 1996; Kapoor et al., 2007. Biological activities are markedly enhanced by microbial interactions in the rhizosphere of plants (Kapoor et al., 2007. Many investigators have successfully used mycorrhiza to increase the availability of immobilized phosphate and thus minimize the use of mineral fertilizers. Arbuscular Mycorrhizal Fungi (AMF can better enable a plant to withstand environmental stresses such as drought and salinity. AMF interacts with pathogens and other rhizosphere inhabitants which affect plant health and nutrition. More importantly, mycorrhizal fungi are capable of dissolving weakly soluble soil minerals, especially phosphate, by releasing acids or increasing CO2 partial pressure (Gupta et al., 2002; Gosling et al., 2006; Kapoor et al., 2007. Therefore, they have the ability to enhance host plant uptake of relatively immobile nutrients particularly P, S and Zn. Limited water supply is also another major environmental constraint in the productivity of crop and medicinal plants. Moisture deficiency induces various physiological and metabolic responses such as stomatal closure, decline in growth rate and photosynthesis (Flexas and Medrano, 2002. The results of Baher et al. (2002 showed that greater soil water stress decreased plant height and total fresh and dry weight of Satureja hortensis. Materials and Methods In order to study the effects of mycorrhiza inoculation and different irrigation levels on the growth, quantitative and qualitative yield of fennel (Foeniculum vulgare Mill. and ajwain (Trachyspermum ammi L., a field experiment was conducted as factorial based on randomized

  16. 和田茴香籽中黄酮类化合物提取工艺研究%Optimization of the extration of technology of the flavonoids from the seeds of Foeniculum vulgare Hotan

    Institute of Scientific and Technical Information of China (English)

    毛居代·亚尔买买提; 朱江梅; 原慧; 布海力期木·伊米提

    2011-01-01

    采用正交设计法对新疆和田茴香籽中的黄酮类化合物提取工艺参数进行优化.结果表明:最佳提取工艺为70 ml 60%乙醇在70℃下提取2.5h.本实验采用分光光度法,以芦丁标准品测定新疆和田茴香籽提取物中总黄酮的含量,总黄酮含量为1.58%.该工艺稳定、简便、快速,可作为检测茴香籽提取物中黄酮含量的一种手段.%To optimize the parameters of the flavonoids from seeds of fennel by orthogonal design. The optimum extraction technology was 70℃and60%ethanol70 ml, extract time 2. 5 h. The total flavonoids contents are assayed by using spectroscopic methods with rutin as standard. The flvonoids content is 1. 58% at the optimum condition. This extraction technology is stable, repid, reproducible and suitable for the determination of total flavonoids in seeds of foeniculum vulgare mill.

  17. 比较研究新疆小茴香不同溶剂提取物的抗氧化活性%Comparison of antioxidant activity of different solvent extracts of Foeniculum vulgare Mill from Xinjiang

    Institute of Scientific and Technical Information of China (English)

    王强; 殷超; 彭秧

    2012-01-01

    研究新疆小茴香4种不同溶剂萃取物的抗氧化活性.采用DPPH、ABTS和还原能力法评估不同萃取物的抗氧化活性,并测定了萃取物中的总酚和总黄酮的含量.结果表明,各样品具有不同程度的抗氧化能力,甲醇萃取物的抗氧化活性显著强于其他溶剂萃取物.小茴香抗氧化活性的主要物质为酚类化合物,与其总黄酮的含量并没有明显的关联.%The antioxidant activity of four different solvent extracts of Foeniculum vulgare Mill were determined by three different test systems, DPPH, ABTS and reducing power assay. The contents of total phenolics and total flavonoids of different extracts were also tested. The result shows that samples had different antioxidant ability; the antioxidant of methanol extract was significantly higher than those of the others. The main substances of antioxidant activities of F. vulgare Mill is phenolic compounds, and had no obvious correlation with the quantity of total flavonoids of extracts.

  18. Accurate Mass GC/LC-Quadrupole Time of Flight Mass Spectrometry Analysis of Fatty Acids and Triacylglycerols of Spicy Fruits from the Apiaceae Family

    Science.gov (United States)

    Nguyen, Thao; Aparicio, Mario; Saleh, Mahmoud A.

    2016-01-01

    The triacylglycerol (TAG) structure and the regio-stereospecific distribution of fatty acids (FA) of seed oils from most of the Apiaceae family are not well documented. The TAG structure ultimately determines the final physical properties of the oils and the position of FAs in the TAG molecule affects the digestion; absorption and metabolism; and physical and technological properties of TAGs. Fixed oils from the fruits of dill (Anethum graveolens), caraway (Carum carvi), cumin (Cuminum cyminum), coriander (Coriandrum sativum), anise (Pimpinella anisum), carrot (Daucus carota), celery (Apium graveolens), fennel (Foeniculum vulgare), and Khella (Ammi visnaga), all from the Apiaceae family, were extracted at room temperature in chloroform/methanol (2:1 v/v) using percolators. Crude lipids were fractionated by solid phase extraction to separate neutral triacylglycerols (TAGs) from other lipids components. Neutral TAGs were subjected to transesterification process to convert them to their corresponding fatty acids methyl esters (FAMES) using 1% boron trifluoride (BF3) in methanol. FAMES were analyzed by gas chromatography-quadrupole time of flight (GC-QTOF) mass spectrometry. Triglycerides were analyzed using high performance liquid chromatography-quadrupole time of flight (LC-QTOF) mass spectrometry. Petroselinic acid was the major fatty acid in all samples ranging from 57% of the total fatty acids in caraway up to 82% in fennel. All samples contained palmitic (16:0), palmitoleic (C16:1n-9), stearic (C18:0), petroselinic (C18:1n-12), linoleic (C18:2n-6), linolinic (18:3n-3), and arachidic (C20:0) acids. TAG were analyzed using LC-QTOF for accurate mass identification and mass spectrometry/mass spectrometry (MS/MS) techniques for regiospesific elucidation of the identified TAGs. Five major TAGs were detected in all samples but with different relative concentrations in all of the tested samples. Several other TAGs were detected as minor components and were present in

  19. Accurate Mass GC/LC-Quadrupole Time of Flight Mass Spectrometry Analysis of Fatty Acids and Triacylglycerols of Spicy Fruits from the Apiaceae Family.

    Science.gov (United States)

    Nguyen, Thao; Aparicio, Mario; Saleh, Mahmoud A

    2015-12-02

    The triacylglycerol (TAG) structure and the regio-stereospecific distribution of fatty acids (FA) of seed oils from most of the Apiaceae family are not well documented. The TAG structure ultimately determines the final physical properties of the oils and the position of FAs in the TAG molecule affects the digestion; absorption and metabolism; and physical and technological properties of TAGs. Fixed oils from the fruits of dill (Anethum graveolens), caraway (Carum carvi), cumin (Cuminum cyminum), coriander (Coriandrum sativum), anise (Pimpinella anisum), carrot (Daucus carota), celery (Apium graveolens), fennel (Foeniculum vulgare), and Khella (Ammi visnaga), all from the Apiaceae family, were extracted at room temperature in chloroform/methanol (2:1 v/v) using percolators. Crude lipids were fractionated by solid phase extraction to separate neutral triacylglycerols (TAGs) from other lipids components. Neutral TAGs were subjected to transesterification process to convert them to their corresponding fatty acids methyl esters (FAMES) using 1% boron trifluoride (BF₃) in methanol. FAMES were analyzed by gas chromatography-quadrupole time of flight (GC-QTOF) mass spectrometry. Triglycerides were analyzed using high performance liquid chromatography-quadrupole time of flight (LC-QTOF) mass spectrometry. Petroselinic acid was the major fatty acid in all samples ranging from 57% of the total fatty acids in caraway up to 82% in fennel. All samples contained palmitic (16:0), palmitoleic (C16:1n-9), stearic (C18:0), petroselinic (C18:1n-12), linoleic (C18:2n-6), linolinic (18:3n-3), and arachidic (C20:0) acids. TAG were analyzed using LC-QTOF for accurate mass identification and mass spectrometry/mass spectrometry (MS/MS) techniques for regiospesific elucidation of the identified TAGs. Five major TAGs were detected in all samples but with different relative concentrations in all of the tested samples. Several other TAGs were detected as minor components and were present in

  20. Accurate Mass GC/LC-Quadrupole Time of Flight Mass Spectrometry Analysis of Fatty Acids and Triacylglycerols of Spicy Fruits from the Apiaceae Family

    Directory of Open Access Journals (Sweden)

    Thao Nguyen

    2015-12-01

    Full Text Available The triacylglycerol (TAG structure and the regio-stereospecific distribution of fatty acids (FA of seed oils from most of the Apiaceae family are not well documented. The TAG structure ultimately determines the final physical properties of the oils and the position of FAs in the TAG molecule affects the digestion; absorption and metabolism; and physical and technological properties of TAGs. Fixed oils from the fruits of dill (Anethum graveolens, caraway (Carum carvi, cumin (Cuminum cyminum, coriander (Coriandrum sativum, anise (Pimpinella anisum, carrot (Daucus carota, celery (Apium graveolens, fennel (Foeniculum vulgare, and Khella (Ammi visnaga, all from the Apiaceae family, were extracted at room temperature in chloroform/methanol (2:1 v/v using percolators. Crude lipids were fractionated by solid phase extraction to separate neutral triacylglycerols (TAGs from other lipids components. Neutral TAGs were subjected to transesterification process to convert them to their corresponding fatty acids methyl esters (FAMES using 1% boron trifluoride (BF3 in methanol. FAMES were analyzed by gas chromatography-quadrupole time of flight (GC-QTOF mass spectrometry. Triglycerides were analyzed using high performance liquid chromatography-quadrupole time of flight (LC-QTOF mass spectrometry. Petroselinic acid was the major fatty acid in all samples ranging from 57% of the total fatty acids in caraway up to 82% in fennel. All samples contained palmitic (16:0, palmitoleic (C16:1n-9, stearic (C18:0, petroselinic (C18:1n-12, linoleic (C18:2n-6, linolinic (18:3n-3, and arachidic (C20:0 acids. TAG were analyzed using LC-QTOF for accurate mass identification and mass spectrometry/mass spectrometry (MS/MS techniques for regiospesific elucidation of the identified TAGs. Five major TAGs were detected in all samples but with different relative concentrations in all of the tested samples. Several other TAGs were detected as minor components and were present in some

  1. 维药小茴香根皮乙酸乙酯部位化学成分研究%study on chemical constituents of ethyl acetate portion of roots of Foeniculum vulgare Mill

    Institute of Scientific and Technical Information of China (English)

    宋凤凤; 美丽万·阿不都热依木

    2014-01-01

    To study on chemical constituents of the ethyl acetate extract part of roots of FOeNiculum vulGare Mill. Ethyl acetate extract part were isolated and pured by silica gel column chromatography,Sephadex LH-20 and recrystallization,their chemical structures were elucidated by spectral data analysis( 1 H NMR and 13 C NMR). Result showed that five compounds were isolated from the ethyl acetate extract part,they were identified as dillapiol(1),butyl isobutyl phthalate(2),3R,8S 9Z-falcarindiol(3),β-sitosterol(4), linoleic acid( 5 ). This research provides basic understanding about the medicinal values of F. vulGare roots.%采用硅胶柱色谱、Sephadex LH-20和重结晶等分离手段对维药小茴香根皮中乙酸乙酯提取部分进行分离纯化,通过1 H NMR、13 C NMR进行结构鉴定。结果表明,从乙酸乙酯提取部分分离5个化合物,分别鉴定为莳萝脑(1)、邻苯二甲酸丁基异丁基二酯(2)、镰叶芹二醇(3)、β-谷甾醇(4)、甘油酸(5)。该研究为了解维药茴香根皮的药用价值提供了研究基础。

  2. Fumigant Activities of Essential Oil from Foeniculum vulgare against Rhizopertha dominica (Fab.) Adults at Different Temperatures%温度对小茴香精油熏杀谷蠹成虫活性的影响

    Institute of Scientific and Technical Information of China (English)

    黄衍章; 李世广; 王小云

    2011-01-01

    采用滤纸栽药熏蒸法,测试了不同温度处理条件下小茴香精油在空仓和实仓两种模拟条件下对谷蠹成虫的熏杀活性.结果表明,小茴香精油在空仓模拟条件下对谷蠹成虫的熏杀活性好于实仓.在30℃条件下,小茴香精油6.67 μL/L空仓处理试虫48 h、100.00 μL/L实仓处理谷蠹成虫7d,试虫的校正死亡率均为100%.此外,小茴香精油对谷蠹子代种群也具有较强的控制作用.%The fumigant activities of essential oil from Foeniculum vulgare seeds against adults of Rhizopertha dominie a (Fab.) were tested by drug-paper method in simulation warehouses. When the pests were not fed with wheat kernels, the F. vulgare essential oil showed better fumigant activities than those fed with wheat kernels. When the non-fed pests were exposure by drug vapor with 6.67 μL/L for 48 h and pests fed with wheat were exposure by drug vapor with 100.00 μL/L for 7 d at 30 t, the corrected mortalities of R. dominica were both 100%. Furthermore, the F. vulgare essential oil could effectively restrain the R. dominica of next generation.

  3. Determination of 20 elements in Foeniculum vulgare Mill by microwave digestion and ICP-AES%微波辅助消解ICP-AES法测定小茴香中20种元素

    Institute of Scientific and Technical Information of China (English)

    陈燕芹; 刘红

    2014-01-01

    采用微波消解法处理小茴香样品,电感耦合等离子体发射光谱( ICP-AES)测定其中Na、K、Sr、Ca、Mg、P、As、Zn、Pb、Co、Cd、Ni、Ba、Fe、Mn、Cr、Cu、Al、Ti 和 B 20种常量和微量元素的含量。20种元素的检出限为0.00002~0.00468μg·mL-1,回收率为89.50%~107.89%,相对标准偏差( RSD)均小6%。结果显示,小茴香果实及其嫩叶中除含有人体必需的常量元素K,Na,Ca,Mg,P外,还含有Fe、Zn、Mn、Cr、Co等必需的微量元素和其他元素Ti、B、Ni、Al、Sr、Ba等。%The contents of 20 elements of Na,K,Sr,Ca,Mg,P,As,Zn,Pb,Co,Cd,Ni,Ba,Fe,Mn,Cr,Cu,Al,Ti and B in Foenicu-lum vulgare Mill were determined by ICP-AES,and the samples were pretreated by microwave digestion method. The detection limits were 0. 00002~0. 00468 μg·mL-1 ,and the recovery rates were 89. 50% ~107. 89%,the precisions were less than 6%. Five mac-roelements,K,Na,Ca,Mg and P,five essential microelements,Fe,Zn,Mn,Cr and Co,and seven other elements,Ti,B,Ni,Al,Sr and Ba were detected in all samples.

  4. Analysis on changes of chemical compounds in different samples of fried Foeniculum vulgare%民族药小茴香炮制前后化学成分的变化分析

    Institute of Scientific and Technical Information of China (English)

    张帆; 李臻; 田树革; 马丽

    2009-01-01

    Objective: To analysis the changes of chemical compounds in fried Foeniculum vulgare. Method: Cleaned F. vulgare were fried with honey, Pharbitis nil, salt solution, vinegar, wine and bran, respectively, according to different Chinese medicine frying theories. Different volatile ingredints were extracted from fried products, and their contents and physical constants were detected.Gas chromatography-mass spectrometry (GC-MS) was developed for analyzing the changes of chemical compounds in different samplesof fried F. vulgare. Result: The experimental results showed that the content of volatile oil in F. vulgare decreased after been fried.Among these effective ingredients in fried samples, trans-anethole was the ingredient of the maximum content, and the contents of all twenty-four ingredinets had changed. Furthermore, other eighteen compounds were created; Among them, linalylacetate, farnesene,p-allyiphenyl aromatic oxide, menthone, and hexyl octanoate were detected firstly in F. vulgare. Conclusion: GC-MS is effective to fleetly analyse the frying changes of herbs flectly.%目的:研究小茴香经不同方法炮制后化学成分的动态变化.方法:应用不同炮制原理对净制后的小茴香分别进行蜜制、黑牵牛制、"四"制和麸制,使用标准挥发油提取器提取炮制品中挥发油成分,检测其含量和物理常数,并利用毛细管气相色谱-电子轰击质谱联用仪对其进行分析.结果:小茴香经不同辅料炮制后挥发油含量显著降低;炮制前后反式-茴香脑含量均最高,生品中24种化合物经不同方法炮制后含量均发生了变化,且发生了转化,共新产生了18种化合物,其中乙酸里那酯、法尼烯、对-烯苯基芳香醚、薄荷酮和辛酸己酯为首次在小茴香药材中检测到.结论:扩展了气相色谱-质谱联用技术的应用范围,适用于对药材炮制变化的快速分析.

  5. Extraction of the Volatile Compounds in Foeniculum Vulgare Mill. Using Ultrasonic Nebulization Extraction Coupled with Solid-phase Extraction%小茴香中挥发性成分的超声雾化-固相萃取研究

    Institute of Scientific and Technical Information of China (English)

    于翠; 杨金娟; 魏洪敏; 师宇华

    2015-01-01

    利用超声雾化-固相萃取法萃取,气相色谱法和气相色谱-质谱法测定了小茴香中的挥发油.采用正十四烷作为洗脱溶剂,体积为50μL、吹扫气流速为200 mL/min,吹扫时间为5 min.利用优化后的方法测定了小茴香中挥发性成分的种类和含量,与水蒸馏提取法所得结果相似.测定样品溶液中挥发性成分的回收率为65.8%~102.0%,相对标准偏差小于8.0%.%The ultrasonic nebulization extraction coupled with solid-phase extraction ( UNE-SPE) followed by gas chromatography and gas chromatography-mass spectrometry was developed and applied to extract and determine the vol-atile compounds from foeniculum vulgare mill.Experimental parameters, including the kind of extraction solvent ( n-tetradecane), solvent volume (50μL), flow rate of purging gas (200 mL/min) and purging time (5 min), were ex-amined and optimized.The contents of the constituents from foeniculum vulgare mill obtained by the proposed method were closed to those obtained by hydro-distillation ( HD) .The recoveries for determining the four constituents are be-tween 65.8%~102.0%and RSDs are less than 8.0%.

  6. Antifungal activity of essential oils against selected terverticillate penicillia

    Directory of Open Access Journals (Sweden)

    Soňa Felšöciová

    2015-02-01

    Full Text Available The aim of this study was to screen 15 essential oils of selected plant species, viz. [i]Lavandula angustifolia[/i], [i]Carum carvi[/i], [i]Pinus mungo var. pulmilio[/i], [i]Mentha piperita[/i], [i]Chamomilla recutita[/i] L.,[i] Pinus sylvestris[/i], [i]Satureia hortensis[/i] L., [i]Origanum vulgare[/i] L., [i]Pimpinella anisum[/i], [i]Rosmarinus officinali[/i]s L., [i]Salvia officinalis[/i] L., [i]Abietis albia etheroleum[/i], [i]Chamomilla recutita[/i] L. [i]Rausch[/i], [i]Thymus vulgaris[/i] L., [i]Origanum vulgare[/i] L. for antifungal activity against five [i]Penicillium[/i] species: [i]Penicillium brevicompactum[/i], [i]Penicillium citrinum[/i], [i]Penicillium crustosum[/i], [i]Penicillium expansum[/i] and [i]Penicillium griseofulvum[/i]. The method used for screening included the disc diffusion method. The study points out the wide spectrum of antifungal activity of essential oils against [i]Penicillium[/i] fungi. There were five essential oils of the 15 mentioned above which showed a hopeful antifungal activity: [i]Pimpinella anisum[/i], [i]Chamomilla recutita[/i] L., [i]Thymus vulgaris[/i], [i]Origanum vulgare[/i] L. The most hopeful antifungal activity and killing effect against all tested penicillia was found to be [i]Origanum vulgare[/i] L. and [i]Pimpinella anisum[/i]. The lowest level of antifungal activity was demonstrated by the oils [i]Pinus mungo var. pulmilio[/i], [i]Salvia officinalis[/i] L., [i]Abietis albia etheroleum[/i], [i]Chamomilla recutita[/i] L.[i] Rausch[/i], [i]Rosmarinus officinalis[/i].

  7. Insecticidal, repellent and oviposition-deterrent activity of selected essential oils against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus.

    Science.gov (United States)

    Prajapati, Veena; Tripathi, A K; Aggarwal, K K; Khanuja, S P S

    2005-11-01

    Essential oils extracted from 10 medicinal plants were evaluated for larvicidal, adulticidal, ovicidal, oviposition-deterrent and repellent activities towards three mosquito species; Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus. The essential oils of Juniperus macropoda and Pimpinella anisum were highly effective as both larvicidal and ovicidal. The essential oil of P. anisum showed toxicity against 4th instar larvae of A. stephensi and A. aegypti with equivalent LD95 values of 115.7 microg/ml, whereas it was 149.7 microg/ml against C. quinquefasciatus larvae. Essential oils of Zingiber officinale and Rosmarinus officinalis were found to be ovicidal and repellent, respectively towards the three mosquito species. The essential oil of Cinnamomum zeylanicum resulted into highest repellent (RD95) values of 49.6, 53.9 and 44.2 mg/mat against A. stephensi, A. aegypti and C. quinquefasciatus, respectively apart from oviposition-deterrent potential.

  8. Screening of Iranian plants for antifungal activity: Part 2

    Directory of Open Access Journals (Sweden)

    "Amin Gh.R

    2002-08-01

    Full Text Available In this study, 278 species from 37 families of native Iranian plants were screened for in vitro antifungal activity against 19 fungal strains. Initially, the crude extracts in concentration of 100 μg/ml were tested. Among 278 plant extracts, 201(71.27% of them showed antifungal activity against at least one fungal strain. A wide range of total extracts of different species were shown to have potentially noticeable antifungal effects. The outstanding species were: Mentha longifolia, Saliva multicaulis, Thymus transcaspicus, Zataria multiflora, Glycyrrhiza glabra, Hulthemia persica, Heracleum persicum, Pimpinella anisum, Pragnos ferulacea, Pragnos uloptera, and Viola odorata.

  9. Aromatic compound glucosides, alkyl glucoside and glucide from the fruit of anise.

    Science.gov (United States)

    Fujimatu, Eiko; Ishikawa, Toru; Kitajima, Junichi

    2003-07-01

    From the polar portion of the methanolic extract of the fruit of anise (Pimpinella anisum L.), which has been used as a spice and medicine since antiquity, four aromatic compound glucosides, an alkyl glucoside and a glucide were isolated together with 24 known compounds. The structures of the new compounds were clarified as (E)-3-hydroxyanethole beta-D-glucopyranoside, (E)-1'-(2-hydroxy-5-methoxyphenyl)propane beta-D-glucopyranoside, 3-hydroxyestragole beta-D-glucopyranoside, methyl syringate 4-O-beta-D-glucopyranoside, hexane-1,5-diol 1-O-beta-D-glucopyranoside and 1-deoxy-L-erythritol 3-O-beta-D-glucopyranoside by spectral investigation.

  10. Bactericidal activity of black pepper, bay leaf, aniseed and coriander against oral isolates.

    Science.gov (United States)

    Chaudhry, Nazia Masood Ahmed; Tariq, Perween

    2006-07-01

    Present investigation focused on antibacterial potential of aqueous decoction of black pepper (Piper nigrum L.), bay leaf (Laurus nobilis L.), aniseed (Pimpinella anisum L.), and coriander (Coriandum sativum L.) against 176 bacterial isolates belonging to 12 different genera of bacterial population isolated from oral cavity of 200 individuals. The disc diffusion technique was employed. Overall aqueous decoction of black pepper was the most bacterial-toxic exhibited 75% antibacterial activity as compared to aqueous decoction of bay leaf (53.4%) and aqueous decoction of aniseed (18.1%), at the concentration of 10 ml/disc. The aqueous decoction of coriander did not show any antibacterial effect against tested bacterial isolates.

  11. Water-soluble constituents of anise: new glucosides of anethole glycol and its related compounds.

    Science.gov (United States)

    Ishikawa, Toru; Fujimatu, Eiko; Kitajima, Junichi

    2002-11-01

    From the water-soluble portion of the methanolic extract of the fruit of anise (Pimpinella anisum L.), which has been used as a spice and medicine since antiquity, twelve new and five known glucosides of phenylpropanoids, including four stereoisomers of anethole glycol 2'-O-beta-D-glucopyranoside and four stereoisomers of 1'-(4-hydroxyphenyl)propane-1',2'-diol 2'-O-beta-D-glucopyranoside were isolated together with anethole glycols and guaiacyl glycerol. The structures of the new compounds were clarified by spectral investigation.

  12. Foeniculum vulgare Mill (Umbelliferae) Attenuates Stress and ...

    African Journals Online (AJOL)

    Abstract. Purpose: To evaluate the anti-stress and memory-enhancing properties of F. vulgare extract in ... Conclusion: F. vulgare may be useful in the management of stress and stress-related disorders on account of ..... Kutlu HR, Forbes JM.

  13. 维药小茴香抗肝纤维化作用及对TGF-β/smad信号转导通路的影响%Effects of foeniculum vulgare mill of anti liver ifbrosis on TGF-β/smad signal pathways

    Institute of Scientific and Technical Information of China (English)

    张泽高; 肖琳; 詹欣宇; 银皓; 鲁晓擘; 张跃新

    2014-01-01

    目的:探讨小茴香提取物抗肝纤维化的作用及其对TGF-β/smad信号转导通路和肝星状细胞活化的影响。方法选择44只SD大鼠给予40%四氯化碳(CCl4)橄榄油溶液4 ml/(kg·体重)皮下注射5周制备肝纤维化模型,成模后随机分成治疗组和模型对照组,同时取6只正常大鼠做空白对照。治疗组给予小茴香提取物灌胃,模型对照组给予生理盐水灌胃,4周末处死大鼠检测血清ALT、AST、HA、LN,肝石蜡切片行Masson染色和抗α平滑肌动蛋白(α-SMA)、TGF-β受体Ⅰ型(TGF-βRⅠ)、TGF-β1免疫组织化学染色,反转录实时荧光定量PCR分析肝组织TGF-β1、smad2 mRNA相对内参基因甘油醛-3-磷酸脱氢酶(GAPDH)的表达量。结果小茴香提取物治疗组血清ALT、AST、HA水平,肝组织内胶原纤维含量、α-SMA、TGF-βRⅠ、TGF-β1表达以及TGF-β1、smad2 mRNA相对表达量均明显低于模型对照组。结论小茴香提取物可通过抑制TGF-β/smad信号转导通路抑制肝星状细胞活化,从而减轻大鼠肝纤维化。%Objective To evaluate the therapeutic effects of Foeniculum Vulgare Mill in liver ifbrosis rats, and to investigate how it work on TGF-β/smad signal pathways and hepatic stellate cell activation. Methods Total of fifty male SD rats were randomly divided into 3 groups:a blank control group (BC group, 6 rats), a hepatic ifbrosis model control group (HFC group, 22 rats), a foeniculum vulgare mill treatment group (FVMT group, 22 rats). Forty-four rats of HFC group and FVMT group were given 40%CCl4 olive oil mixture by subcutaneous injection to make hepatic ifbrosis models for 5 weeks. Rats in FVMT group were given FVMT by intragastric administration, while rats in HFC group were treated with saline for weeks and then were sacriifced. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), hyaluronic acid (HA), laminin (LN) were detected. Histopathologic changes

  14. Aqueous two phase system and ultrasonic wave coextraction of total flavonoids from Foeniculum vulgare stems and study on its antioxidation efficacy%超声波协同双水相体系提取茴香茎中总黄酮及其抗氧化性研究

    Institute of Scientific and Technical Information of China (English)

    贾贵华; 王金辉; 任雪峰

    2012-01-01

    Uhrasonic extraction technology integrated with ethanol and ammonium sulfate aqueous two-phase separation was used to extracl total flavnnoids from Foen,iculum vulgare stem. Based on single factor and orthogonal experiment, the optimum extracting conditions were as follows: 50% of ethanol, solid to liquid ratio of 1:15 and ultrasonic extracting for 20 rain. The order of factors to affect total flawmes extraction was: ethanol concentration〉ultrasonic time〉solid to liquid ratio. Determination showed thai ifs antioxidation efficacv was enhanced with the increasing of its concentration.%将超声波技术和双水相体系萃取技术综合应用于提取茴香茎中总黄酮。通过单因素试验和正交试验相结合,得出影响茴香茎中黄酮提取因素的主要次序为提取液浓度〉提取时间〉料液比。最佳提取工艺条件为乙醇体积分数50%、料液比1:15、超声时间20min。抗氧化能力检测表明,该提取物抗氧化性显著,且浓度越高,其抗氧化性越强。

  15. Greek plant extracts exhibit selective estrogen receptor modulator (SERM)-like properties.

    Science.gov (United States)

    Kassi, Eva; Papoutsi, Zoi; Fokialakis, Nikolaos; Messari, Ioanna; Mitakou, Sophia; Moutsatsou, Paraskevi

    2004-11-17

    To prevent bone loss that occurs with increasing age, nutritional and pharmacological factors are needed. Traditional therapeutic agents (selective estrogen receptor modulators or SERMs, biphosphonates, calcitonin) may have serious side effects or contraindications. In an attempt to find food components potentially acting as SERMs, we submitted four plant aqueous extracts derived from Greek flora (Sideritis euboea, Sideritis clandestina, Marticaria chamomilla, and Pimpinella anisum) in a series of in vitro biological assays reflective of SERM profile. We examined their ability (a) to stimulate the differentiation and mineralization of osteoblastic cell culture by histochemical staining for alkaline phosphatase and Alizarin Red-S staining, (b) to induce, like antiestrogens, the insulin growth factor binding protein 3 (IGFBP3) in MCF-7 breast cancer cells, and (c) to proliferate cervical adenocarcinoma (HeLa) cells by use of MTT assay. Our data reveal that all the plant extracts studied at a concentration range 10-100 microg/mL stimulate osteoblastic cell differentiation and exhibit antiestrogenic effect on breast cancer cells without proliferative effects on cervical adenocarcinoma cells. The presence of estradiol inhibited the antiestrogenic effect induced by the extracts on MCF-7 cells, suggesting an estrogen receptor-related mechanism. In conclusion, the aqueous extracts derived from Sideritis euboea, Sideritis clandestina, Marticaria chamomilla, and Pimpinella anisum may form the basis to design "functional foods" for the prevention of osteoporosis.

  16. Burdur Yöresi Ballarının Polen Analizi

    Directory of Open Access Journals (Sweden)

    Deniz TAŞKIN

    2009-07-01

    Full Text Available 2004–2005 yılları arasında Burdur ili ve ilçelerinden 20 bal örnegi toplanmıs ve bu örneklerde polen analizi Lieux (1972 ve Maurizio (1951' ya göre Sorkun (1989 tarafından belirtilen yöntemle yapılmıstır. Mikroskobik analiz sonuçlarında 33 farklı familyaya ait 58 takson teshis edilmistir. Centaurea, Compositae, Brassicaceae, Rosaceae, Pimpinella anisum ve Anthriscus taksonları çoğu istasyonda poleni görülen taksonlardır. Dominant polenler, Apiaceae, Pimpinella anisum, Anthriscus, Cardamine, Compositae, Centaurea, Ericaceae ve Dianthus taksonlarına; sekonder polenler, Brassicaceae, Fabaceae, Crepis, Xeranthemum ve Trifolium taksonlarına aittir. İncelenen örnekler içerisinde sadece Burdur( Merkez'dan alınan örnekte dominant polen (Cardamine ve eser polenler (Compositae, Centaurea, Fabaceae, Polygonum, Anthriscus, Scandix, Poaceae, Populus, Rosaceae, Laurus nobilis, Malvaceae, Liliaceae, Caryopyllaceae, Ficaria bulundugu için unifloral (tek çiçek kaynaklı bal olarak belirlenmistir. Geriye kalan 19 bal örneği multifloral bal olarak tanımlanmıstır.

  17. 铅处理对茴香植株生长及精油组分的影响%Effect of Pb on growth and essential oil components of fennel (Foeniculum vulgare L.) plant

    Institute of Scientific and Technical Information of China (English)

    肖艳辉; 何金明; 潘春香; 张振明; 淡豫

    2014-01-01

    为了揭示铅对茴香(Foeniculum vulgare L.)生长和次生代谢产物-精油组分的影响及茴香植株对铅的吸收累积特性,本试验采用水培方法,在霍格兰营养液中加入铅,设计0(对照)、1 mg·L-1、5 mg·L-1、10 mg·L-1处理,研究了不同铅质量浓度对茴香植株生长、精油组分及茴香植株对铅吸收累积的影响。结果表明:不同铅质量浓度处理对茴香植株的形态、生物量、色素质量分数、碳和氮质量分数、铅的吸收累积及精油组分均有影响。随着铅质量浓度的增加,茴香植株的株高呈降低趋势,且各处理之间差异显著,真叶数大致也呈降低趋势,但仅10 mg·L-1处理显著低于对照,各处理的最大叶长差异均不显著,最大叶宽仅对照与5 mg·L-1处理之间差异显著。随着铅质量浓度的增加,地上部鲜质量和干质量均呈逐渐降低趋势,且铅处理后的地上部鲜质量和干质量均显著低于对照,地下部鲜质量和干质量呈逐渐升高趋势,但铅处理后的地下部鲜质量和干质量与对照差异均不显著,根冠比呈逐渐增加趋势,但仅10 mg·L-1处理显著高于对照。随着铅质量浓度的增加,叶绿素a、叶绿素b、叶绿素a+b和类胡萝卜素质量分数均呈降低趋势,且均低于对照,叶绿素a/叶绿素b以5 mg·L-1处理最高,但仅显著高于1 mg·L-1处理。铅处理后,可溶性蛋白质质量分数均高于对照,但仅1 mg·L-1处理和5 mg·L-1处理显著高于对照,全氮质量分数与可溶性蛋白质质量分数的变化趋势基本一致,但各处理之间差异均不显著;随铅质量浓度的增加,可溶性糖质量分数呈降低趋势,除1 mg·L-1处理显著高于对照外,其余铅处理均显著低于对照,各处理的全碳质量分数与对照差异均不显著。随铅质量浓度的增加,茴香植株对铅的吸收累积显著增加。不同铅质

  18. The antimicrobial activity of essential oils and extracts of some medicinal plants grown in Ash-shoubak region - South of Jordan.

    Science.gov (United States)

    Abu-Darwish, Mohammad Sanad; Al-Ramamneh, Ezz Al-Dein Muhammed; Kyslychenko, Viktoria Sergeevna; Karpiuk, Uliana Vladimirovna

    2012-01-01

    The inhibitory effects of essential oils as well as chloroformic extracts of Thymus vulgaris, Thymus serpyllum, Salvia officinalis and Pimpinella anisum grown in Ash-shoubak region-south of Jordan and their possible individual phytochemical constituents was screened against pathogenic clinical and standard strains of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. The bioassay employed was the agar well diffusion method. The essential oils and chloroformic extracts of T. vulgaris and T. serpyllum were the most effective against the tested strains of bacteria. Clinical and standard strains of S .aureus and P. aeruginosa were uninhibited by S. officinalis essential oils. P. aeruginosa tested strains were also resistant to P. anisum essential oils. For almost all bacterial strains, the highest antibacterial effect of oils was obtained with the highest tested dose (15 μl). Chlorformic extracts of S. officinalis showed small activity against standard and clinical E. coli strains and were not effective to inhibit strains of P. aeruginosa and S. aureus. Chloroformic extracts obtained from P. anisum and applied at 300 μg/cm(2) slightly inhibited E. coli, but moderately inhibited S. aureus. It is shown from the results that the antibacterial effects of the individual components varied depending upon their chemical structure, functional groups and configuration as well as doses used. This study showed the beneficial effects of the essential oils of T. serpyllum and T. vulgaris grown in Ash-shoubak in inhibiting the growth of microbes and the implications this could have in pharmacy and food technology.

  19. Solanum carolinense L. en Pimpinella peregrina L. in Nederland

    NARCIS (Netherlands)

    Dirkse, Gerard M.; Holverda, Wout J.; Hochstenbach, Sophie M.H.; Reijerse, Fons A.I.

    2007-01-01

    Solanum carolinense L., een kniehoog bestekeld en met sterharen bezet, bleekblauw bloeiend struikje is recent op een aantal plekken aan vooral de Waal gevonden. De eerste vondst dateert echter al van 1983 bij Ochten, maar de soort werd toen niet herkend. Gebleken is dat de populaties zich in ieder g

  20. Umbelliferae Familyasından Bazı Önemli Kültür Türlerinin Isparta Ekolojik Koşullarında Tarımsal ve Teknolojik Özelliklerinin Belirlenmesi

    Directory of Open Access Journals (Sweden)

    Sevil KESKİN

    2016-04-01

    Full Text Available Anason (Pimpinella anisum L., dereotu (Anethum graveolens L., kimyon (Cuminum cyminum L., kişniş (Coriandrum sativum L., rezene (Foeniculum vulgare Mill. dünyada ve ülkemizde kültürü yapılan çok değerli tıbbi ve aromatik bitkilerdir. Bu araştırmada; anason, kimyon, kişniş, rezene ve dereotu türlerine ait çeşitlerin ve populasyonların Isparta ekolojik koşullarında bazı tarımsal ve teknolojik özelliklerinin belirlenmesi, uçucu yağ, sabit yağ ve petroselinik asit kaynağı olarak değerlendirme olanaklarının araştırılması amaçlanmıştır. Tarla ve laboratuvar araştırmalarından elde edilen sonuçlara göre, en yüksek tohum verimi anasonda “Yeşilova” populasyonundan (47.01 kg/da, dereotunda “Akşehir” populasyonundan (77.75 kg/da, kimyonda “Egebir09” çeşidinden (97.00 kg/da, kişnişte “Erbaa” çeşidinden (82.85 kg/da ve rezenede “Denizli” populasyonundan (63.66 kg/da elde edilmiştir. En yüksek uçucu yağ oranı anasonda “Burdur” populasyonunda (%3.60, dereotunda “Burdur” populasyonunda (%3.73, kimyonda “Türkmen09” çeşidinde (%2.13, kişnişte “Gamze” çeşidinde (%0.47 ve rezenede “Burdur” populasyonunda (%3.13 tespit edilmiştir. En önemli ana uçucu yağ bileşeni anasonda trans-anetol (%95.56-95.88, dereotunda D-karvon (%37.87-67.76, kimyonda a-thujenal (%30.90-42.52, kişnişte linalool (%81.85-88.94 ve rezenede trans-anetol (%79.67-89.13 olarak bulunmuştur. En yüksek sabit yağ oranı anasonda “Burdur” populasyonunda (%30.17, dereotunda “Burdur” populasyonunda (%21.24, kimyonda “Egebir09” çeşidinde (%29.03, kişnişte “Erbaa” çeşidinde (%25.42 ve rezenede “Denizli” populasyonunda (%23.67 belirlenmiştir. En yüksek petroselinik asit oranı ise anasonda “Çardak” populasyonunda (%63.37, dereotunda “Burdur” populasyonunda (%87.28, kimyonda “Türkmen09” çeşidinde (%60.18, kişnişte “Erbaa” çeşidinde (%79.89 ve

  1. Efeito de sistemas de consórcio e inseticida na formação dos estômatos em plântulas de erva-doce (Foeniculum vulgare Mill. Effects of intercropping systems and insecticide in formation of stomata in fennel seedlings

    Directory of Open Access Journals (Sweden)

    C.F. Azevedo

    2012-01-01

    Full Text Available Foeniculum vulgare Mill., pertencente à família Apiacea, é conhecida como erva-doce e apresenta grande importância medicinal e comercial, tanto no Brasil como em vários outros países. Objetivou-se com esta pesquisa, estudar o desenvolvimento dos estômatos em plântulas de F. vulgare oriundas de sementes produzidas em sistemas de consórcio erva-doce X algodão e com aplicação do inseticida monocrotofós. A erva-doce foi cultivada em consórcio com algodão colorido cultivar BRS Safira, sendo utilizados os seguintes tratamentos: 1A2E, uma fileira de algodão e duas de erva-doce; 2A1E, duas fileiras de algodão e uma de erva-doce; ES, erva-doce solteira; onde foram distribuídos com e sem aplicação de inseticida, totalizando seis tratamentos. As sementes colhidas foram semeadas em areia e mantidas em casa de vegetação por 25 dias. Partes das plântulas (zona de transição, caule, cotilédones e folhas foram seccionadas à mão livre, coradas e montadas em lâminas com glicerina para observação em microscópio. Foram avaliadas as seguintes características: número de estômatos, diâmetro polar e equatorial dos estômatos e número de cloroplastos nas células-guarda. Os dados foram analisados em delineamento inteiramente casualizado e distribuídos em arranjo fatorial 3X2; sendo realizado teste de Tukey a 5% de probabilidade. Na zona de transição e no caule observou-se aumento do número e do diâmetro polar dos estômatos quando foram utilizados sistemas de consórcio. Nos cotilédones, a erva-doce solteira proporcionou maior número de estômatos, porém com menor diâmetro e com menor quantidade de cloroplastos. Já na folha, os consórcios influenciaram positivamente o número de estômatos e de cloroplastos. De forma geral, os sistemas de consórcio e o inseticida influenciaram positivamente o desenvolvimento dos estômatos das plântulas de erva-doce.Foeniculum vulgare Mill., belonging to the family Apiaceae, is known as

  2. EFFECTS OF CO2 CONCENTRATION ON PLANT GROWTH, CONTENTS ANDCOMPONENTS OF ESSENTIAL OIL IN FENNEL (FOENICULUM VULGARE)%CO2浓度升高对茴香植株生长、精油含量和组分的影响

    Institute of Scientific and Technical Information of China (English)

    任安祥; 何金明; 肖艳辉; 王羽梅

    2008-01-01

    采用每日定时向密封人工气候室补充CO2的方法,研究了3种CO2浓度(平均浓度分别为287.11、532.88和780.46 μmol·mol-1)对茴香(Foeniculum vulgare)生长、精油含量和组分的影响.结果表明:随着CO2浓度的升高,茴香的株高、花序数、花序鲜重、花序干重、全株干重和植株的干物率均有所上升;植株可溶性糖和全碳含量不断升高,而全氮和蛋白氮含量不断减少;叶色素含量呈下降趋势,叶绿素a/b比的差异不显著;植株精油含量(分别为1.26、1.45和1.57ml·(100g)-1 DW)和单株精油产量(分别为0.019、0.023和0.033ml)均随之升高.从茴香植株的精油中鉴定出22种成分,用不同浓度的CO2处理,精油的成分种类没有差异,成分相对含量却有差别,差异达到极显著水平的有:α-蒎烯、β-蒎烯、月桂烯、对聚伞花素、反式葑醇乙酸酯和顺式茴香脑;含量差异达到显著水平的有:香桧烯、水芹烯、罗勒烯、γ-萜品烯、3,4-二甲基.2,4,6-三烯、爱草脑、葑醇乙酸酯、古巴烯、金合欢烯和吉玛烯.茴香精油的主要成分反式茴香脑的含量(分别为55.94%、57.20%和59.55%)随着CO2浓度的升高而升高,而柠檬烯含量(29.60%、30.24%和26.12%)表现出相反的趋势,二者在不同的CO2浓度处理之间差异均不显著.

  3. 小茴香精油对肝纤维化大鼠抗氧化应激损伤作用的研究%Antioxidant effects of the Uygur herb, Foeniculum Vulgare Mill, in a rat model of hepatic fibrosis

    Institute of Scientific and Technical Information of China (English)

    张泽高; 鲁晓擘; 肖琳; 唐莉; 张丽娟; 张韬; 詹欣宇; 马秀敏; 张跃新

    2012-01-01

    .81,P=0.005),F组与D组比较差异有统计学意义(Z=-2.59P=0.015).结论 小茴香精油具有减轻肝脏炎症反应、防治肝纤维化进展的作用,其机制可能与抗氧化应激损伤有关.%Objective To evaluate therapeutic and antioxidant effects of Uygur Herb Foeniculum Vulgare Mill (FVM) in hepatic fibrosis rats.Method Hepatic fibrosis model was built in rats by subcutaneous injection with 40% CCl4 olive oil mixture.At the same time the rats were given high lipoid-low protein animal feeds for 5 weeks.94 male SD rats were randomly divided into six groups:blank control group (A-group),8 rats were feed in normal; prevention model control group (B-group),10 rats were given saline solution by intragastric administration during make of hepatic fibrosis model; FVM prevention group (C-group),10 rats were given FVM by intragastric administration during make of hepatic fibrosis model; model control group (D-group),FVM treatment group (E-group); Fuzhenghuayu treatment group (F-group).22 rats in each D,E,F-group were respectively given saline solution,FVM and Fuzhenghuayu by intragastric administration after hepatic fibrosis model were built.At the 5-th weekend,A,B,C- group rats were sacrificed.At the 6-th,7-th,8-th,9-th weekend,4-6 rats in D,E,F-group were sacrificed.Serum alanine aminotransferase (ALT),aspartate aminotransferase (AST),hyaluronic acid (HA),laminin (LN) and 8 - hydroxy-2-deoxyguanosine (8-OHdG) were detected,liver tissue homogenate superoxide dismutase (SOD),glutathione peroxidase (GSH-Px),malondialdehyde (MDA) were detected.Histopathologic changes were observed after H.E and Masson staining.The expression of alpha-smooth muscle actin(α-SMA) were detected by immunohistochemical staining.The data were analyzed by SPSS17.0 software.Results The serum levels of ALT,AST,HA,and LN in the FVM prevention group were significantly reduced compared to the prevention model control group.(P < 0.05).Rats in FVM treatment group appeared a marked lower

  4. Antibacterial activity of aromatic plants essential oils from Serbia against the Listeria monocytogenes

    Directory of Open Access Journals (Sweden)

    Klaus Anita

    2009-01-01

    Full Text Available The purpose of this study was to examine the effectiveness of selected essential oils for the control of the growth and survival of pathogenic bacteria Listeria monocytogenes ATCC 19112 and Listeria monocytogenes ATCC 19115, which are of significant importance in food hygiene. Essential oils extracted from Salvia officinalis L., Rosmarinus officinalis L., Majorana hortensis Moench., Thymus vulgaris L., Carum carvi L., Pimpinella anisum L. and Coriandrum sativum L. were evaluated. Antibacterial activity was done by the disk diffusion method in the presence of pure essential oils and four suspensions in alcohol. The best results obtained with Thymus vulgaris and Majorana hortensis essential oils, which were acting microbicidaly on both observed strains of Listeria monocytogenes, even in the small concentration. Because some of the essential oils were highly inhibitory even in small quantities to selected pathogenic bacteria, they may provide alternatives to conventional antimicrobial additives in foods. .

  5. Exposure of Candida to p-anisaldehyde inhibits its growth and ergosterol biosynthesis.

    Science.gov (United States)

    Shreaz, Sheikh; Bhatia, Rimple; Khan, Neelofar; Muralidhar, Sumathi; Basir, Seemi F; Manzoor, Nikhat; Khan, Luqman Ahmad

    2011-01-01

    p-Anisaldehyde (4-methoxybenzaldehyde), an extract from Pimpinella anisum seeds, is a very common digestive herb of north India. Antifungal activity of p-anisaldehyde was investigated on 10 fluconazole-resistant and 5 fluconazole-sensitive Candida strains. Minimum inhibitory concentrations (MIC(90)) ranged from 250 µg/ml to 600 µg/ml for both sensitive and resistant strains. Ergosterol content was drastically reduced by p-anisaldehyde-62% in sensitive and 66% in resistant strains-but did not corelate well with MIC(90) values. It appears that p-anisaldehyde exerts its antifungal effect by decreasing NADPH routed through up-regulation of putative aryl-alcohol dehydrogenases. Cellular toxicity of p-anisaldehyde against H9c2 rat cardiac myoblasts was less than 20% at the highest MIC value. These findings encourage further development of p-anisaldehyde.

  6. Aqueous extracts of some medicinal plants are as toxic as Imidacloprid to the sweet potato whitefly, Bemisia tabaci.

    Science.gov (United States)

    Ateyyat, Mazen A; Al-Mazra'awi, Mohammad; Abu-Rjai, Talal; Shatnawi, Mohamad A

    2009-01-01

    Aqueous extracts of nine plants, known to have medicinal activity, were tested for their toxicity against the sweet potato whitefly, Bemisia tabaci Genn. (Homoptera: Aleurodidae) compared to the toxicity of the insecticide, Imidacloprid. Extracts of Lepidiuim sativum L. (Brassicales: Brassicaceae) killed 71 % of early stage nymphs, which was not significantly different from mortality caused by Imidacloprid. Treatment of pupae with three plant extracts, L. sativum, Achillea biebersteinii L. (Asterales: Asteraceae), or Retama raetam (Forssk.) Webb and Berthel (Fabales: Fabaceae) prevented adult development, and treatment with R. raetam extract killed adults, at levels that were not significantly different from Imidacloprid. None of the other plants showed significant toxicity. However extracts of four plants, Pimpinella anisum L. (Apiales: Apiaceae), Galium longifolium (Sibth. and SM.) (Gentianales: Rubiaceae), R. raetam and Ballota undulata Bentham (Lamiales: Lamiaceae) had a repellent effect.

  7. Microarray analysis of p-anisaldehyde-induced transcriptome of Saccharomyces cerevisiae.

    Science.gov (United States)

    Yu, Lu; Guo, Na; Yang, Yi; Wu, Xiuping; Meng, Rizeng; Fan, Junwen; Ge, Fa; Wang, Xuelin; Liu, Jingbo; Deng, Xuming

    2010-03-01

    p-Anisaldehyde (4-methoxybenzaldehyde), an extract from Pimpinella anisum L. seeds, is a potential novel preservative. To reveal the possible action mechanism of p-anisaldehyde against microorganisms, yeast-based commercial oligonucleotide microarrays were used to analyze the genome-wide transcriptional changes in response to p-anisaldehyde. Quantitative real-time RT-PCR was performed for selected genes to verify the microarray results. We interpreted our microarray data with the clustering tool, T-profiler. Analysis of microarray data revealed that p-anisaldehyde induced the expression of genes related to sulphur assimilation, aromatic aldehydes metabolism, and secondary metabolism, which demonstrated that the addition of p-anisaldehyde may influence the normal metabolism of aromatic aldehydes. This genome-wide transcriptomics approach revealed first insights into the response of Saccharomyces cerevisiae (S. cerevisiae) to p-anisaldehyde challenge.

  8. Fennel (Foeniculum vulgare) provides antioxidant protection for boar semen cryopreservation.

    Science.gov (United States)

    Malo, C; Gil, L; Cano, R; González, N; Luño, V

    2012-05-01

    Boar semen is extremely vulnerable to cold shock and it is also sensitive to peroxidation due to the high content of unsaturated fatty acids in the plasma membrane. Antioxidants exert a protective effect on the plasma membrane of frozen boar sperm. Fennel has been shown to contain antioxidant substances. Therefore, this study was performed to evaluate the effect of different concentrations of fennel added to the freezing extender on boar semen quality and lipid peroxidation after thawing. Semen collected from four boars was cryopreserved in lactose-egg-yolk extender or in the same extender with varying concentration of fennel essences: low (LF); medium (MF); high (HF). Analysis of data clearly indicated that higher concentrations of fennel produced significant improvement in total motility. Moreover, when fennel was included in the extender, a dose-dependent tendency to increase sperm viability was observed. In contrast, the addition of fennel had no effect on acrosome integrity or hypoosmotic swelling test (HOST) compared with the control. Malondialdehyde (MDA) formation decreased significantly in fennel groups, yielding similar results for MF and HF. Fennel seems a new antioxidant for use in sperm cryopreservation, but its particular effects on sperm physiology must be further studied, especially the causes of motility stimulation and its effect on lipoxidation.

  9. Direct somatic embryogenesis from protoplasts of Foeniculum vulgare.

    Science.gov (United States)

    Miura, Y; Tabata, M

    1986-08-01

    Protoplasts prepared from an embryogenic cell suspension culture of fennel gave rise to somatic embryoids directly through unequal cell divisions of enlarged, ellipsoidal cells, when embedded in hormone-free LS agarose medium. On the other hand, protoplasts embedded in LS agarose medium containing 2,4-D and kinetin proliferated through unpolarized cell divisions to form calli, which gave somatic embryoids on the surface upon transfer onto the same medium. In either case, somatic embryoids germinated to develop into normal plantlets when cultured on hormone-free LS agar medium under illumination.

  10. 钾浓度对茴香植株生长发育、精油含量和组分的影响%The effect of potassium concentrations on growth, essential oil contents and components of fennel plant (Foeniculum vulgare Mill.)

    Institute of Scientific and Technical Information of China (English)

    何金明; 肖艳辉; 王羽梅; 潘春香

    2013-01-01

      为了揭示钾浓度对植物生长发育和次生代谢产物——精油累积的影响特性及其内在关系,采用水培方法,以日本园试配方中钾含量为标准1K,设计1/8K、1/4K、1/2K、1K和2K处理,研究了不同钾浓度对茴香Foeniculum vulgare Mill.生长发育、精油含量和组分的影响。结果表明:不同钾浓度处理对植株形态(茴香分枝数、节数和花序数)、生物量(地上部鲜质量、全株鲜质量和全株干质量)、碳、氮含量(全氮、蛋白氮、全碳、可溶性糖、C/N)、精油含量、单株精油产量和色素含量(Chl.a、Chl.b、CAR.、Chl.a+b、Chl.a/b)影响显著,综合认为1K为利于茴香初生代谢产物累积(生长发育)的最佳处理,而1/4K、1/8K和1K为利于次生代谢产物累积(精油含量)的较好处理;相关分析表明,精油含量与全碳含量(r=0.72)、可溶性糖含量(r=0.70)成极显著正相关;单株精油产量与全氮含量(r=0.49)呈显著正相关。不同钾浓度处理茴香精油均鉴定出23种成分,其中17种成分的相对含量差异显著。精油第一主要成分反式-茴香脑含量为74.75%~79.15%,第二主要成分柠檬烯含量为8.25%~14.81%,第三主要成分莳萝芹菜脑相对含量2.04%~3.82%,处理间均差异显著。结论:钾浓度不但影响茴香的生长发育,还影响着茴香次生代谢产物精油的含量、产量和成分组成比例;适宜的钾浓度(1K)可以实现茴香初生代谢产物累积(生长发育)与次生代谢产物累积(精油产量)均高的结果。%The objective of this study was to reveal the response characteristics and the internal correlation of fennel plant growth and essential oil accumulation to potassium concentrations. Effect of different potassium concentrations to fennel plant growth, essential oil content and components was studied by using method of solution culture, and making

  11. ATIVIDADE INSETICIDA DE PLANTAS MEDICINAIS SOBRE O Callosobruchus maculatus (COLEOPTERA: BRUCHIDAE

    Directory of Open Access Journals (Sweden)

    Antonia Mirian Nogueira de Moura Guerra

    2009-01-01

    Full Text Available This study aimed to evaluate the insecticidal activity of eight medicinal plants on Callosobruchus maculatus (F. (Coleoptera: Bruchidae. Plant powder from Rosmarinus officinalis (L. leaves, Peumus boldus (Mol leaves, Matricaria chamomilla (L. flowers, Baccharis trimera (Less. leaves, Camellia sinensis (L. leaves, Thea sinensis (L. leaves, Ilex paraguariensis (St. Hil. leaves, and fruits of Pimpinella anisum (L. were used in the experiment. Bioassays were carried out under constant conditions of temperature (28±2 °C, relative humidity (70±5% and scotophase of 24 hours. Experimental units consisted of 20 grams of cowpea (Vigna unguiculata (L. Walp. grains with 13% wet basis moisture content, 2 g plant powder and ten adult insects, both placed in plastic containers (60 mm in diameter x 60 mm high. The analyzed variables included: insect mortality, oviposition (egg number and instantaneous rate of population growth (ri. The number of dead insects was recorded 5 days after bioassays were started, the egg number was recorded after 12 days, and ri was estimated on the 60th day. All the tested plants had toxicity to C. maculatus. The highest mortality was found in insects treated with P. boldus and I. paraguariensis. Number of eggs and ri also varied significantly among the powders. The lowest mean values for these parameters were recorded in the plots treated with P. boldus, P. anisum and R. officinalis.

  12. The inhibitory effect of eupatorium japonicum thunb and foeniculum vulgare extract on prostatic hyperpla-sia in rats%泽兰与小茴香提取物抑制大鼠前列腺增生的实验研究

    Institute of Scientific and Technical Information of China (English)

    唐维; 代光成; 薛波新; 单玉喜; 张文芳

    2014-01-01

    能显著抑制大鼠前列腺增生,其作用机制在一定程度上与降低大鼠血清睾酮、双氢睾酮含量及睾酮/雌二醇值有关。%Objective Benign prostatic hyperplasia ( BPH) is one of common diseases in aged males , and searching for new therapeutic drugs to BPH has been a research hotspot in recent years .This article was to study the inhibitory effect of eupatorium ja-ponicum thunb and foeniculum vulgare extract ( EFE) on benign prostatic hyperplasia in rats and its possible mechanism . Methods 48 male rats were randomly divided into 6 groups:normal control group without any treatment , model group of BPH treated with subcu-taneous injection of testosterone propionate , positive control group of BPH treated with dutasteride , high, middle and low dosage groups according to different EFE dosage (156 mg/kg, 234 mg/kg and 312 mg/kg).45 days after the treatment, the rats were sacrificed for measurement of the prostate glandular wet weight , the index of prostate gland ( PI ) , the morphological changes of prostate gland by light microscopy and the content of sex hormone . Results The prostate wet weight and PI decreased after EFE treatment for 45 days compared with the BPH model group(P<0.01 ).The hyperplastic glandular epithelium papilla waned and even disappeared in three EFE groups under the light microscope , and the epithelial cells became cubical or flat .High dosage EFE group (312 mg/kg) has simi-lar efficacy to dutasteride group .EFE significantly reduced serum testosterone content , dihydrotestosterone content and T/E2 ratio( P<0.05 ). Conclusion EFE can significantly inhibit prostatic hyperplasia in rats , and its mechanism is related to the decrease of the contents of serum testosterone and dihydrotestosterone as well as T/E2 ratio.

  13. Effect of ethnomedicinal plants used in folklore medicine in Jordan as antibiotic resistant inhibitors on Escherichia coli

    Directory of Open Access Journals (Sweden)

    Aburjai Talal A

    2010-02-01

    Full Text Available Abstract Background Escherichia coli occurs naturally in the human gut; however, certain strains that can cause infections, are becoming resistant to antibiotics. Multidrug-resistant E. coli that produce extended-spectrum β lactamases (ESBLs, such as the CTX-M enzymes, have emerged within the community setting as an important cause of urinary tract infections (UTIs and bloodstream infections may be associated with these community-onsets. This is the first report testing the antibiotic resistance-modifying activity of nineteen Jordanian plants against multidrug-resistant E. coli. Methods The susceptibility of bacterial isolates to antibiotics was tested by determining their minimum inhibitory concentrations (MICs using a broth microdilution method. Nineteen Jordanian plant extracts (Capparis spinosa L., Artemisia herba-alba Asso, Echinops polyceras Boiss., Gundelia tournefortii L, Varthemia iphionoides Boiss. & Blanche, Eruca sativa Mill., Euphorbia macroclada L., Hypericum trequetrifolium Turra, Achillea santolina L., Mentha longifolia Host, Origanum syriacum L., Phlomis brachydo(Boiss. Zohary, Teucrium polium L., Anagyris foetida L., Trigonella foenum-graecum L., Thea sinensis L., Hibiscus sabdariffa L., Lepidium sativum L., Pimpinella anisum L. were combined with antibiotics, from different classes, and the inhibitory effect of the combinations was estimated. Results Methanolic extracts of the plant materials enhanced the inhibitory effects of chloramphenicol, neomycin, doxycycline, cephalexin and nalidixic acid against both the standard strain and to a lesser extent the resistant strain of E. coli. Two edible plant extracts (Gundelia tournefortii L. and Pimpinella anisum L. generally enhanced activity against resistant strain. Some of the plant extracts like Origanum syriacum L.(Labiateae, Trigonella foenum- graecum L.(Leguminosae, Euphorbia macroclada (Euphorbiaceae and Hibiscus sabdariffa (Malvaceae did not enhance the activity of

  14. Propriedade antibacteriana de óleos essenciais de especiarias sobre bactérias contaminantes de alimentos Antibacterial property of spice essential oils on food contaminating bacteria

    Directory of Open Access Journals (Sweden)

    Vinicius Nogueira Trajano

    2009-09-01

    Full Text Available As especiarias são conhecidas por exercerem uma estabilidade frente à ação de microrganismos, estando inseridas no grupo dos alimentos estáveis. Esta propriedade conservante das especiarias está relacionada com a presença de compostos antibacterianos na sua composição. Tais compostos podem ser usados no combate a bactérias deteriorantes de alimentos, por esse motivo, este trabalho visa à investigação da propriedade antimicrobiana dos óleos essenciais de Cinnamomum zeylanicum Blume, Coriandrum sativum L., Cuminum cyminum L., Mentha. piperita L., Ocimum basilicum L., Origanum majorana L., Pimpinella anisum L., Piper nigrum L., Rosmarinus officinalis L. e Zingiber officinalis Rosc. frente a bactérias contaminantes de alimentos. Para esse estudo, foram selecionadas 10 cepas bacterianas deteriorantes de alimentos. Para a realização dos ensaios antibacterianos, foi utilizado o método de difusão em meio sólido. Os resultados mostram que os óleos essenciais das folhas do E. caryophyllata e da C. zeylanicum mostraram uma eficiência na inibição de todas as cepas bacterianas ensaiadas. Os outros óleos produziram atividade antibacteriana, porém com menor espectro de ação. A pesquisa de tais produtos mostra-se bastante promissora, sendo essa uma boa alternativa para a substituição de aditivos químicos em alimentos.Spices are known for their stability against microbial action; that being so, they are included in the group of stable foods. This preservative property of spices is related to the presence of antibacterial compounds in their composition. Such compounds could be used to control the food contaminating bacteria. This study aimed to assess the antimicrobial property of the essential oils from Cinnamomum zeylanicum Blume, Coriandrum sativum L., Cuminum cyminum L., Mentha. piperita L., Ocimum basilicum L., Origanum majorana L., Pimpinella anisum L., Piper nigrum L., Rosmarinus officinalis L., and Zingiber officinalis Rosc

  15. Comparison of antimicrobial activity of herbs & spices and their phytochemical determination

    Directory of Open Access Journals (Sweden)

    Manoj Kumar Singh

    2011-01-01

    Full Text Available The antimicrobial activities of crude methanolic extracts of five spices and herbs viz. Cuminum Cyminum (Family: Apiaceae, Nigella sativa (Family: Ranunculaceae, Pimpinella anisum (Family: Apiaceae, Trachyspermum copticum (Family: Umbelliferae, and rhizome of Zingiber officinale (Family: Zingiberaceae were investigated by agar well diffusion method against gram-positive bacteria (Bacillus amyloliquefaciens and Staphylococcus aureus and gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa. The antimicrobial activities of the extracts were assessed by the diameter of zone of inhibition against test strains. The extract of N. sativa showed the best inhibition against S. aureus and P. aeruginosa. The extract of Z. officinale and P. anisum showed same minimum inhibitory concentration (MIC against all the test strains. The extract showed significant inhibitory activity against B. amyloliquefaciens, P. aeruginosa, and S. aureus. The extract of C. cyminum significantly inhibited S. aureus, B. amyloliquefaciens, and P. aeruginosa. The extract of T. copticum was effective against E. coli, B. amyloliquefaciens, and P. aeruginosa, while the highest MIC obtained against S. aureus. The diameters of zone of inhibition obtained for standard antibiotics viz. ampicillin, erythromycin, and tetracycline at MIC values of the extracts were compared with the crude methanolic extracts and found equally or more effective against the test strains. Phytochemical analysis showed carbohydrates, inulin, alkaloids, and glycosides in all the five extracts. Flavonoids, terpenoids, tannins, reducing sugars, soluble phenols, and saponin glycosides were also detected. Steroids were absent. The organoleptic properties of the extracts were determined. The study indicated the great potential of these extracts as effective antimicrobial agents.

  16. Allelopatic effects of some medicinal plant essential oils on plant seeds germination

    Directory of Open Access Journals (Sweden)

    ALI SHOKOUHIAN

    2016-04-01

    Full Text Available The effect of essential oils from some medicinal plants on seed germination was studied with the aim of assessing their potential use as bioherbicides. The experiment was conducted as factorial based on completely randomized design (CRD with three replications. Seeds of 3 summer crops including lettuce (Lactuca sativa, pepper (Piper longum and tomato (Solanum lycopersicum were exposed to essential oils of rosemary (Rosmarinus officinalis, thyme (Thymus vulgaris and anise (Pimpinella anisum at 3 different concentrations (25 and 50% diluted and undiluted. Treated seeds were grown in a growth chamber at 25°C for 5 days. The number of germinated seeds in each Petri dish was daily counted. After five days seed germination percentage (Ge was calculated. Biplot analysis was performed using genotype plus genotype environment interaction (GGE method. Results showed that the allelopathic effect on Ge was varied among studied plants, which was mainly due to i differences in the composition of the studied essential oils and ii different allelopathic effects of the studied essential oils on Ge. Accordingly, compared to the individual use, combining several essential oils would have a greater inhibitory effect on Ge of weeds.

  17. Antimicrobial activities of six essential oils commonly used as condiments in Brazil against Clostridium perfringens

    Directory of Open Access Journals (Sweden)

    Marcela Radaelli

    2016-06-01

    Full Text Available Abstract Despite recent advances in food production technology, food-borne diseases (FBD remain a challenging public health concern. In several countries, including Brazil, Clostridium perfringens is among the five main causative agents of food-borne diseases. The present study determines antimicrobial activities of essential oils of six condiments commonly used in Brazil, viz., Ocimum basilicum L. (basil, Rosmarinus officinalis L. (rosemary, Origanum majorana L. (marjoram, Mentha × piperita L. var. Piperita (peppermint, Thymus vulgaris L. (thyme and Pimpinella anisum L. (anise against C. perfringens strain A. Chemical compositions of the oils were determined by GC–MS (gas chromatography–mass spectrometry. The identities of the isolated compounds were established from the respective Kováts indices, and a comparison of mass spectral data was made with those reported earlier. The antibacterial activity was assessed from minimum inhibitory concentration (MIC and minimum bactericidal concentration (MBC using the microdilution method. Minimum inhibitory concentration values were 1.25 mg mL-1 for thyme, 5.0 mg mL-1 for basil and marjoram, and 10 mg mL-1 for rosemary, peppermint and anise. All oils showed bactericidal activity at their minimum inhibitory concentration, except anise oil, which was only bacteriostatic. The use of essential oils from these common spices might serve as an alternative to the use of chemical preservatives in the control and inactivation of pathogens in commercially produced food systems.

  18. Antimicrobial activities of six essential oils commonly used as condiments in Brazil against Clostridium perfringens.

    Science.gov (United States)

    Radaelli, Marcela; da Silva, Bárbara Parraga; Weidlich, Luciana; Hoehne, Lucélia; Flach, Adriana; da Costa, Luiz Antonio Mendonça Alves; Ethur, Eduardo Miranda

    2016-01-01

    Despite recent advances in food production technology, food-borne diseases (FBD) remain a challenging public health concern. In several countries, including Brazil, Clostridium perfringens is among the five main causative agents of food-borne diseases. The present study determines antimicrobial activities of essential oils of six condiments commonly used in Brazil, viz., Ocimum basilicum L. (basil), Rosmarinus officinalis L. (rosemary), Origanum majorana L. (marjoram), Mentha × piperita L. var. Piperita (peppermint), Thymus vulgaris L. (thyme) and Pimpinella anisum L. (anise) against C. perfringens strain A. Chemical compositions of the oils were determined by GC-MS (gas chromatography-mass spectrometry). The identities of the isolated compounds were established from the respective Kováts indices, and a comparison of mass spectral data was made with those reported earlier. The antibacterial activity was assessed from minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using the microdilution method. Minimum inhibitory concentration values were 1.25mgmL(-1) for thyme, 5.0mgmL(-1) for basil and marjoram, and 10mgmL(-1) for rosemary, peppermint and anise. All oils showed bactericidal activity at their minimum inhibitory concentration, except anise oil, which was only bacteriostatic. The use of essential oils from these common spices might serve as an alternative to the use of chemical preservatives in the control and inactivation of pathogens in commercially produced food systems. Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.

  19. The influence of fertilization on yield of caraway, anise and coriander in organic agriculture

    Directory of Open Access Journals (Sweden)

    Aćimović Milica G.

    2013-01-01

    Full Text Available Many plants of Apiaceae family have long been well known because of flavorful aromatic spice and, because of its healing properties, are often used in folk medicine and in cooking. In our study three plants of this family were included: caraway (Carum carvi L., anise (Pimpinella anisum L. and coriander (Coriandrum sativum L.. Regarding good agro-ecological conditions for growing these plants in Serbia, and a new world trend of increasing organic agricultural production, the aim of this study was to investigate the effects of application of various types of fertilizers on yield of studied plant species. The field experiment was carried out during the years of 2011 and 2012, in three localities in Vojvodina Province with the local ecotypes and six different treatments (control, ‘Slavol’, ‘Bactofil B-10’, ‘Royal Ofert’, vermicompost and NPK. The highest caraway yield was obtained by the application of biofertilizer ‘Bactofil B-10’. As regards anise and coriander the highest yield was achieved by the application of chemical fertilizer. The most effective organic fertilizers were the following: vermicompost for anise and specific poultry manure ‘Royal Ofert’ granules for coriander.

  20. Evaluation of Effects of Zingiber officinale on Salivation in Rats

    Directory of Open Access Journals (Sweden)

    Goli Chamani

    2011-06-01

    Full Text Available There are some herbal plants in Iranian traditional system of medicine which are believed to be excellent remedies to alleviate the symptoms of xerostomia. The aim of the present study was to evaluate the effect of systemic administration of seven different herbal extracts on the rate of salivation in rats. The extracts of 7 herbs; Zingiber officinale Roscoe (Zingiberaceae, Citrus sinensis (L. Osbeck (Rutaceae, Artemisia absinthium L. (Asteraceae, Cichorium intybus L. (Asteraceae, Pimpinella anisum L.(Apiaceae, Portulaca oleracea L.(Portulacaceae, Tribulus terrestris L. (Zygophyllaceae were prepared. Nine groups of animals (including negative and positive control groups were used and seven rats were tested in each group. After the injection of extracts, saliva volume was measured gravimetrically in four continuous seven-minute intervals. The results showed that after injection of ginger extracts salivation was significantly higher as compared to the negative control group and other herbal extracts in all of the four intervals (P<0.01. The peak action of the ginger was during the first 7-minute interval and following this, salivation decreased to some extent. The present study suggests that the extract of Zingiber offiicianle can increase the rate of salivation significantly in animal model. Further investigations on different constituents of ginger seem to be essential to identify the responsible constituent for stimulation of saliva secretion.

  1. Utilisation of gamma radiation of Cobalt-60 as quarantine treatment of medicinal plant, aromatic and seasoning plants dehydrated infested by Lasioderma serricorne (Fabricius, 1792) (Coleoptera, Anobiidae) and Plodia interpunctella (Hubner, 1813) (Lepidoptera, Pyralidae); Utilizacao da radiacao gama do Cobalto-60 como tratamento quarentenario de plantas medicinais, aromaticas e condimentares desidratadas infestadas por Lasioderma serricorne (Fabricius, 1792) (Coleoptera, Anobiidae) e Plodia interpunctella (Hubner, 1813) (Lepidoptera, Pyralidae)

    Energy Technology Data Exchange (ETDEWEB)

    Alves, Juliana Nazare

    2007-07-01

    The research had as objective the use of the gamma radiation of the Cobalto-60 as quarantine treatment of the medicinal plant, aromatic and seasoning plants dehydrated infested by Lasioderma serricorne and Plodia interpunctella determining the disinfestation doses to attend the criterion in the not emergency of adults of the species in study and analysing through the Chromatography of Thin Layer the effect of the gamma radiation of the cobalto-60 on the active principle of extract dehydrated of Chamomilla recutita, Pimpinella anisum, Origanum vulgare, Cymbopogon citratus, Ocimum basilicum and Thymus vulgaris. The work was developed in the Laboratorio de Inseticidas in the Instituto Biologico in Sao Paulo in the period of August of 2005 the June of 2007. The radiation source used gamma was an experimental irradiator of Cobalto-60, model Gamacell 220, located in the Instituto de Pesquisas Energeticas e Nucleares - IPEN, located in Sao Paulo. In the period of 30 days after the irradiation of the samples evaluated the number of adults emerged of Lasioderma serricorne and Plodia interpunctella, using the data of mortality for the analysis of Probit. Obtained 100% of not emergency of adults in the Lasioderma serricorne with the dose of 2,00 kGy and 100% of not emergency of adults in the Plodia interpunctella with the dose of 2,25 kGy. The Chromatographic Analysis of Thin Layer was to evaluate did not show chemical differences in the extracts analysed. (author)

  2. Ethnobotanical survey of the medicinal flora used by the Caribs of Guatemala.

    Science.gov (United States)

    Girón, L M; Freire, V; Alonzo, A; Cáceres, A

    1991-09-01

    An ethnobotanical survey was conducted among the Carib population of Guatemala in 1988-1989. In general terms, the sample surveyed possessed a relatively good standard of living. Results indicated that health services were utilized by the population, and that domestic medicine, mainly plants (96.9%) was used by 15% of the population. One hundred and nineteen plants used for medicinal purposes were collected, of which 102 (85.7%) could be identified; a list of these together with the information provided for each plant is presented. The most frequently reported plants used as medicine are: Acalypha arvensis, Cassia alata, Cymbopogon citratus, Melampodium divaricatum. Momordica charantia, Neurolaena lobata, Ocimum basilicum, Petiveria alliacea and Solanum nigrescens. Most of these plants are found in the region, but some are brought from the Highlands or outside of the country, such as Malva parviflora, Matricaria chamomilla, Peumus boldus, Pimpinella anisum, Rosmarinus officinalis and Tagetes lucida. This survey demonstrated that the Carib population of Guatemala has survived in a transcultural environment of African and native Amerindian beliefs.

  3. Efficacy of Iranian Traditional Medicine in the Treatment of Epilepsy

    Directory of Open Access Journals (Sweden)

    Mehri Abdollahi Fard

    2013-01-01

    Full Text Available Epilepsy is a brain disorder which affects about 50 million people worldwide. Ineffectiveness of the drugs in some cases and the serious side effects and chronic toxicity of the antiepileptic drugs lead to use of herbal medicine as a form of complementary and alternative medicine. In this review modern evidences for the efficacy of antiepileptic medicinal plants in Traditional Iranian Medicine (TIM will be discussed. For this purpose electronic databases including PubMed, Scopus, Sciencedirect, and Google Scholar were searched for each of the antiepileptic plants during 1970-February 2013.Anticonvulsant effect of some of the medicinal plants mentioned in TIM like Anacyclus pyrethrum, Pimpinella anisum, Nigella sativa, and Ferula gummosa was studied with different models of seizure. Also for some of these plants like Nigella sativa or Piper longum the active constituent responsible for antiepileptic effect was isolated and studied. For some of the herbal medicine used in TIM such as Pistacia lentiscus gum (Mastaki, Bryonia alba (Fashra, Ferula persica (Sakbinaj, Ecballium elaterium (Ghesa-al Hemar, and Alpinia officinarum (Kholanjan there is no or not enough studies to confirm their effectiveness in epilepsy. It is suggested that an evaluation of the effects of these plants on different epileptic models should be performed.

  4. Application of poly(dimethylsiloxane) fiber sol-gel coated onto NiTi alloy electrodeposited with zirconium oxide for the determination of organochlorine pesticides in herbal infusions.

    Science.gov (United States)

    Budziak, Dilma; Martendal, Edmar; Carasek, Eduardo

    2008-08-01

    A PDMS fiber sol-gel coated onto an NiTi alloy previously electrodeposited with zirconium oxide (named NiTi-ZrO(2)-PDMS) was applied to the determination of organochlorine pesticides (OCPs) in infusions of peppermint (Mentha piperita L.), lemon grass (Cymbopogon citratus Stapf), chamomile (Matricaria recutita L.), lemon balm (Melissa officinalis L.), and anise seeds (Pimpinella anisum L.). Salting-out effect, extraction time, and extraction temperature were optimized firstly by means of a full-factorial design and then using a Doehlert matrix. No salt addition and 50 min of extraction at 70 degrees C were the optimum conditions. Satisfactory LODs in the range of 2-17 ng/L, as well as good correlation coefficients (at least 0.9981) in the linear range studied, were obtained. Calibration was successfully applied using an infusion of M. recutita L. and recovery tests were performed to ensure the accuracy of the method, with values in the range of 77-120%. Comparison of the NiTi-ZrO(2)-PDMS with commercially available PDMS fibers showed that the proposed fiber has an extraction efficiency comparable to that of PDMS 30 microm for the compounds evaluated, demonstrating its potential applicability.

  5. LC–MS/MS based-comparative study of (S-nicotine metabolism by microorganisms, mushroom and plant cultures: Parallels to its mammalian metabolic fate

    Directory of Open Access Journals (Sweden)

    Amira R. Khattab

    2015-12-01

    Full Text Available The metabolic fate of nicotine using the cell cultures of microorganisms, mushroom and plants was explored using LC–MS/MS analysis. The study demonstrated parallels to phase I mammalian metabolism of nicotine and reported that nicotine was biotransformed into its N-oxide by Streptomyces fradiae culture. Moreover, it was metabolized in Pleurotus ostreatus culture into nornicotine, norcotinine and β-nicotyrine; whereas, cotinine and its 3′-hydroxylated derivative were the identified nicotine metabolic products in Pimpinella anisum cell culture. However, the microbial culture of Agaricus bisporus bioconverted nicotine into one of its derived-carcinogenic nitrosamines which is “4-(methylnitrosamino-4-(3-pyridylbutanal”. Mushroom and plant cell cultures were thus proven to be competent to microbial cultures in bioconverting nicotine into many of its previously reported metabolites. Convincingly, the obtained results highlighted the prospect of utilizing other species which are intrinsically-endowed with unique biocatalytic systems, such as mushrooms and plants, in the drug metabolic studies.

  6. Efficacy of Iranian traditional medicine in the treatment of epilepsy.

    Science.gov (United States)

    Abdollahi Fard, Mehri; Shojaii, Asie

    2013-01-01

    Epilepsy is a brain disorder which affects about 50 million people worldwide. Ineffectiveness of the drugs in some cases and the serious side effects and chronic toxicity of the antiepileptic drugs lead to use of herbal medicine as a form of complementary and alternative medicine. In this review modern evidences for the efficacy of antiepileptic medicinal plants in Traditional Iranian Medicine (TIM) will be discussed. For this purpose electronic databases including PubMed, Scopus, Sciencedirect, and Google Scholar were searched for each of the antiepileptic plants during 1970-February 2013.Anticonvulsant effect of some of the medicinal plants mentioned in TIM like Anacyclus pyrethrum, Pimpinella anisum, Nigella sativa, and Ferula gummosa was studied with different models of seizure. Also for some of these plants like Nigella sativa or Piper longum the active constituent responsible for antiepileptic effect was isolated and studied. For some of the herbal medicine used in TIM such as Pistacia lentiscus gum (Mastaki), Bryonia alba (Fashra), Ferula persica (Sakbinaj), Ecballium elaterium (Ghesa-al Hemar), and Alpinia officinarum (Kholanjan) there is no or not enough studies to confirm their effectiveness in epilepsy. It is suggested that an evaluation of the effects of these plants on different epileptic models should be performed.

  7. Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia

    Directory of Open Access Journals (Sweden)

    Snežana PAVLOVIĆ

    2016-12-01

    Full Text Available Anise (Pimpinella anisum L. is an important medicinal spice plant that belongs to the family Apiaceae. Anise seeds are rich in essential oils and this is a reason why anise production in Serbia has increased over the last decade. During a routine health inspection on anise seeds collected from three localities in the province of Vojvodina (Mošorin, Veliki Radinci and Ostojićevo during 2012 and 2013, it was found out that Fusarium spp. were a commonly observed fungi. The presence of Fusarium fungion the seed samples ranged from 3.75-13.75%. The aim of this study was to isolate and identify the strains of Fusarium species present on anise seed samples as it is necessary that commercially used anise seeds are completely free of Fusarium. Based on morphological, microscopic characteristics and a molecular identification by sequencing of TEF gene, the presence of the following species was confirmed on the anise seeds: F. tricinctum, F. proliferatum, F. equiseti, F. oxysporum, F. sporotrichoides, F. incarnatum and F. verticillioides. According to our knowledge and research, this is the first report of F. tricinctum and F. sporotrichoides as pathogens on anise seeds in the world. All seven isolates of Fusarium species are pathogenic to the anise seedlings, while the most virulent species were F. oxysporum, F. tricinctum and F. incarnatum.

  8. Survey on medicinal plants and spices used in Beni-Sueif, Upper Egypt

    Directory of Open Access Journals (Sweden)

    Mohamed Abdelhalim A

    2011-06-01

    Full Text Available Abstract Background This study was conducted to identify medicinal plants and spices used for medicine by the community of Beni-Sueif, Upper Egypt. Methods Ethnobotanical data from local people was collected using direct interviews and a semi-structured questionnaire. Results Forty-eight plant species belonging to twenty-seven families and forty-seven genera were encountered during the study. Their botanical and vernacular names, plant parts used and medicinal uses are given. Results of the study were analyzed using two quantitative tools. The factor informant consensus indicated the agreement in the use of plants and the fidelity level indicated the ratio between the number of informants who independently suggested the use of a species for the same major purpose and the total number of informants who mentioned the plant for any use. The results of the factor informant consensus showed that the cardiovascular category has the greatest agreement, followed by the immunological, gastrointestinal and respiratory categories. The most important species according to their fidelity are: Hibiscus sabdariffa L. for the cardiovascular category; Trigonella foenum-graecum L. for the immunological category; Mentha piperita L. for the gastrointestinal category and Pimpinella anisum L. for the respiratory category. Conclusions Medicinal plants are still used for treatment in Beni-Sueif community despite the availability of prescribed medications. Documentation of this ethnomedicinal knowledge is important. Evaluation of pharmacological activity for the promising medicinal plants is suggested.

  9. Interactions between preparations containing female sex hormones and dietary supplements.

    Science.gov (United States)

    Zabłocka-Słowińska, Katarzyna; Jawna, Katarzyna; Grajeta, Halina; Biernat, Jadwiga

    2014-01-01

    An increasing number of premenopausal women use contraception whereas postmenopausal women use hormone replacement therapy (HRT). This long-term hormone therapy poses a high risk of interactions with dietary supplements. Taking estrogens at the same time as selective estrogen receptor modulators (SERMs), biologically-active compounds of glycine soja, Ginkgo biloba or Pimpinella anisum, may distort the final effect of the hormone agent. On the other hand, estrogen therapy coupled with melatonin or retinol supplementation may lead to an increased level of dietary supplements in the serum as studies have proved a concomitant beneficial effect of HRT and vitamin E supplementation on lipid profiles. In turn, taking preparations containing St John's wort during hormone therapy may lead to a reduction in hormone concentrations in serum and debilitation of the pharmacological effect. It results from the inductive effect of the biologically-active compounds of St John's wort on the metabolism of hormones as a result of the enhanced activity of cytochrome P450 CYP3A4.

  10. Evaluation of effects of Zingiber officinale on salivation in rats.

    Science.gov (United States)

    Chamani, Goli; Zarei, Mohammad Reza; Mehrabani, Mitra; Taghiabadi, Yousef

    2011-01-01

    There are some herbal plants in Iranian traditional system of medicine which are believed to be excellent remedies to alleviate the symptoms of xerostomia. The aim of the present study was to evaluate the effect of systemic administration of seven different herbal extracts on the rate of salivation in rats. The extracts of 7 herbs; Zingiber officinale Roscoe (Zingiberaceae), Citrus sinensis (L.) Osbeck (Rutaceae), Artemisia absinthium L. (Asteraceae), Cichorium intybus L. (Asteraceae), Pimpinella anisum L.(Apiaceae), Portulaca oleracea L.(Portulacaceae), Tribulus terrestris L. (Zygophyllaceae) were prepared. Nine groups of animals (including negative and positive control groups) were used and seven rats were tested in each group. After the injection of extracts, saliva volume was measured gravimetrically in four continuous seven-minute intervals. The results showed that after injection of ginger extracts salivation was significantly higher as compared to the negative control group and other herbal extracts in all of the four intervals (P<0.01). The peak action of the ginger was during the first 7-minute interval and following this, salivation decreased to some extent. The present study suggests that the extract of Zingiber offiicianle can increase the rate of salivation significantly in animal model. Further investigations on different constituents of ginger seem to be essential to identify the responsible constituent for stimulation of saliva secretion.

  11. The Protective Role of Anise Oil in Oxidative Stress and Genotoxicity Produced in Favism.

    Science.gov (United States)

    Koriem, Khaled M M; Arbid, Mahmoud S; El-Gendy, Nadia F

    2016-01-01

    The metabolic disease favism is an acute hemolytic anemia. Anise oil was obtained from Pimpinella anisum L. seeds (family Apiaceae). The objective of this study was to establish the protective effect of anise oil in favism disorders. Forty-eight male albino rats were divided into six groups: group 1 orally administrated 1 mL distilled water, group 2 orally received 300 mg/kg anise oil, and group 3 orally administrated 100 mg/kg anethole over a seven-day period, group 4 favism-induced rats, group 5 orally administrated 300 mg/kg anise oil and group 6 orally administrated 100 mg/kg anethole once a day over a seven-day period prior to favism induction. The result obtained revealed that oral administration of either anise oil or anethole into normal rats over a seven-day period did not induce any change. Following favism induction, hemoglobin, hematocrit, red and white blood cell counts, serum glucose, blood glutathione, glucose-6-phosphate dehydrogenase, total protein, globulin, alanine and aspartate aminotransferases levels were significantly decreased, while serum alkaline phosphatase and bilirubin showed significant increase. Pretreatment with either anise oil or anethole into favism-induced rats prevented these changes. Favism also induced deoxyribonucleic acid (DNA) damage and prior treatment of anise oil maintained liver DNA content. These results were supported by histopathological evaluation. In conclusion, anise oil pretreatment into favism-induced rats decreased the favism disorders, and this effect was related to the anethole ingredient of the oil.

  12. Potencial toxicológico frente Artemia Salina em plantas condimentares comercializadas no município de Campina Grande-PB

    Directory of Open Access Journals (Sweden)

    Emmanuel Moreira Pereira

    2015-03-01

    Full Text Available O conhecimento do potencial toxicológico das plantas é um fator primordial para estipular o limite consumível. Objetivou-se nesse trabalho avaliar o potencial toxicológico de plantas condimentares frente Artemia salina, expostas às concentrações dos extratos das plantas erva-doce (Pimpinella anisum L., pimenta malagueta (Capsicum frutescens, endro (Anethum graveolens L. e alecrim (Rosmarinus officinalis L.. O trabalho foi desenvolvido no Laboratório de Engenharia de Alimentos - LEA pertencente ao Centro de Tecnologia e Recursos Naturais da Universidade Federal de Campina Grande-PB. A erva doce apresentou uma baixa toxicidade, atingindo uma DL50 de 428ppm do extrato. Já a pimenta malagueta exibiu um comportamento similar, atingindo o seu potencial toxicológico máximo na terceira diluição com DL50 716,1ppm. Para o endro, observou-se uma baixa taxa de mortalidade, consequentemente foi considerada não toxica, apresentando a DL50, com 2.624,5ppm. O alecrim apresentou baixos percentuais de mortalidade em náuplios de Artemia, sendo a DL50 com 3.720,6ppm, sendo considerado não toxico. No entanto necessita-se de estudos mais aprofundados para caracterizar a composição química das plantas estudadas e um teste in vivo para as que apresentaram baixa toxicidade.

  13. Antimicrobial activity of Northwestern Mexican plants against Helicobacter pylori.

    Science.gov (United States)

    Robles-Zepeda, Ramón E; Velázquez-Contreras, Carlos A; Garibay-Escobar, Adriana; Gálvez-Ruiz, Juan C; Ruiz-Bustos, Eduardo

    2011-10-01

    Helicobacter pylori is the major etiologic agent of such gastric disorders as chronic active gastritis and gastric carcinoma. Over the past few years, the appearance of antibiotic-resistant bacteria has led to the development of better treatments, such as the use of natural products. This study evaluated the anti-H. pylori activity of 17 Mexican plants used mainly in the northwestern part of Mexico (Sonora) for the empirical treatment of gastrointestinal disorders. The anti-H. pylori activity of methanolic extracts of the plants was determined by using the broth microdilution method. The 50% minimum inhibitory concentrations ranged from less than 200 to 400 μg/mL for Castella tortuosa, Amphipterygium adstringens, Ibervillea sonorae, Pscalium decompositum, Krameria erecta, Selaginella lepidophylla, Pimpinella anisum, Marrubium vulgare, Ambrosia confertiflora, and Couterea latiflora and were greater than 800 μg/mL for Byophyllum pinnatum, Tecoma stans linnaeus, Kohleria deppena, Jatropha cuneata, Chenopodium ambrosoides, and Taxodium macronatum. Only Equisetum gigantum showed no activity against H. pylori. This study suggests the important role that these plants may have in the treatment of gastrointestinal disorders caused by H. pylori. The findings set the groundwork for further characterization and elucidation of the active compounds responsible for such activity.

  14. Application of essential oils in maize grain: impact on Aspergillus section Flavi growth parameters and aflatoxin accumulation.

    Science.gov (United States)

    Bluma, Romina V; Etcheverry, Miriam G

    2008-04-01

    The antifungal activity of Pimpinella anisum L. (anise), Pëumus boldus Mol (boldus), Hedeoma multiflora Benth (mountain thyme), Syzygium aromaticum L. (clove), and Lippia turbinate var. integrifolia (griseb) (poleo) essential oils (EOs) against Aspergillus section Flavi was evaluated in sterile maize grain under different water activity (a(w)) condition (0.982, 0.955, and 0.90). The effect of EOs added to maize grains on growth rate, lag phase, and aflatoxin B(1) (AFB(1)) accumulation of Aspergillus section Flavi were evaluated at different water activity conditions. The five EOs analyzed have been shown to influence lag phase and growth rate. Their efficacy depended mainly on the essential oil concentrations and substrate water activity conditions. All EOs showed significant impact on AFB(1) accumulation. This effect was closely dependent on the water activity, concentration, and incubation periods. Important reduction of AFB(1) accumulation was observed in the majority of EO treatments at 11 days of incubation. Boldus, poleo, and mountain thyme EO completely inhibited AFB(1) at 2000 and 3000 microg g(-1). Inhibition of AFB(1) accumulation was also observed when aflatoxigenic isolates grew with different concentration of EOs during 35 days.

  15. Extraction of Fennel (Foeniculum vulgare) Seeds: Process Optimization and Antioxidant Capacity of the Extracts

    OpenAIRE

    2016-01-01

    This paper presents the study on the extraction of bioactive substances from fennel seeds. The impact of the main process variables (solvent composition, liquid-to-solid ratio, temperature, contact time) on the concentration of the target substances (polyphenols and flavonoids) in the extracts is studied resulting in the selection of a set of operating parameters, at which their content is maximized. Extracts with higher concentration of target compounds demonstrate higher antioxidant capacit...

  16. Essential Oil Extraction of Fennel Seed (Foeniculum vulgare Using Steam Distillation

    Directory of Open Access Journals (Sweden)

    Astrilia Damayanti

    2012-10-01

    Full Text Available Indonesia is a potential country in developing essential oils which is each part of the plants produce essential oils such as leaf, seed, fruit, and root. One of the potential plants is fennel. Fennel oil distillation used fennel seed from Cepogo District, Boyolali Regency. The characteristics of the seed are; the color is black and the length is 0,2 centimeters. The condition operation to exctract of the fennel seed are 1 atm and 7,5 hours. The calculation of the time started when the first fennel oil dropped into the decanter. It finished when the fennel oil was not dropped anymore. The color is bright and muddy. The last process is add 1% (m/m Na2SO4 anhidrous into fennel oil to absorp remain water in it. The distillation process produce fennel oil102,125 grams. Sample of fennel oil tested which are density test, solubility on 90% alcohol, GC-MS test, and AAS test. The result shows that fennel oil from the fennel seed is 2,0425%. The tested samples contain the brightest and the muddies sample. The density of 0,9500 and 0,949 g/cc respectively that is not fulfill to  the Food Chemical Codex (FCC. Samples solubility in 90% alcohol (1:3 is fulfill to the the Food Chemical Codex (FCC.  Three main components of the brightest sample are anethole (47,51%, estragole (22,41%, and  α-fensone (21,92% while the muddiest sample’s components are anethole (52,38%, estragole (21,37%,and α-fensone (15,74%. The AAS test shows that fennel oil contains 65,1473 ppm which does not fulfill the Indonesian National Standards of  patchouli and clove leaf oil. [Keywords— essential oil; extraction; fennel seed; steam distillation

  17. Exchangeable sodium induced changes in yield, water relation and cation composition of fennel (Foeniculum vulgare Mill).

    Science.gov (United States)

    Garg, V K; Singh, P K; Pushpangadan, P

    2005-06-01

    A pot experiment was conducted with the objectives to assess the adaptation potential of fennel crop grown at 10, 20, 25, 35 and 40 ESP (exchangeable sodium percentage) levels. Results showed that the rate of seed germination, plant growth including branching pattern, umbels per plant and 1000 test seed weight were adversely affected by sodic soils. Assuming that fifty percent reduction in seed yield and Na+/K+ ratio in leaf tissue as an index of alkali tolerance revealed that fennel was tolerant up to 25 ESP. The cell sap pH and EC reflected optimum osmoticum maintenance to withstand sodicity stress at this level and beyond this leaf water potential decreased (negatively) more to impede water uptake.

  18. Antimicrobial activity and chemical composition of the essential oils of Portuguese Foeniculum vulgare fruits.

    Science.gov (United States)

    Mota, Ana S; Martins, M Rosário; Arantes, Sílvia; Lopes, Violeta R; Bettencourt, Eliseu; Pombal, Sofia; Gomes, Arlindo C; Silva, Lúcia A

    2015-04-01

    The aim of this study was to investigate the chemical composition and antimicrobial activity of essential oils obtained by hydrodistillation from fruits of six fennel accessions collected from wild populations occurring in the centre and south of Portugal. Composition of essential oils was established by Gas Chromatography-Flame Ionization Detector (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The obtained yields of the essential oils were found to vary greatly in the range of 1.1 to 2.9% (v/w) and the chemical composition varied with the region of collection. A total of 16 compounds were identified. The main compounds were fenchone (16.9 - 34.7%), estragole (2.5 - 66.0%) and trans-anethole (7.9 - 77.7%). The percentages of these three main compounds were used to determine the relationship between the different oil samples and to group them into four different chemotypes: anethole/fenchone; anethole; estragole and anethole/estragole. Antifungal activity of essential oils was evaluated against six food spoilage fungi: Aspergillus niger, A. japonicus, A. oryzae, Fusarium oxysporum, Rhizophus oryzae and R. stolonifer. Antibacterial activity was assessed against three Gram-positive strains: Enterococcus faecalis ATCC 29212, Staphylococcus epidermidis ATCC 12228 and S. aureus ATCC 28213; and against six Gram-negative strains: Escherichia coli ATCC 25922; Morganella morganii LFG 08; Proteus mirabilis LFG 04; Salmonella enteritidis LFG 05; S. entiritidis serovar typhimurium LFG 06 and Pseudomonas aeruginosa ATCC 27853 by the disc diffusion agar method; the minimal inhibitory concentration (MIC) was determined using the broth macro-dilution method. The MIC values varied from 62.5 (E. coli ATCC 25922) to 2000 µmL (P. aeruginosa ATCC 27853).

  19. Effect of Freezing Stress on Electrolyte Leakage of Fennel Foeniculum vulgare

    Directory of Open Access Journals (Sweden)

    a Nezami

    2011-02-01

    Full Text Available Abstract In order to investigation of the effect of freezing stress on electrolyte leakage of two fennel landraces that grown on the field, a factorial experiment based on completely randomized design was conducted with three replications in Agricultural College, Ferdowsi University of Mashhad in 2004. Treatments were two fennel landraces (Khorasan and Kerman, two planting dates and six temperatures (0,-3,-6,-9,-12,-15°C. Seedlings were selected in 4-6 leaf stage from the field and transferred to the laboratory and different organs of seedlings (root, leaf and crown were investigated for cytoplasmice membrane stability. Results indicated that electrolyte leakage percent of fennel landraces and those organs significantly affected by lowering temperature. LT50 in Kerman landrace was lower then Khorasan landrace and LT50 of second planting date was lower then first planting date in two landraces, and LT50 of crown was the lowest. According to the importance of the effect of cold injury on fennel, additional investigations are requested. Keywords: Planting dates, Landrace, Membrane stability, LT50

  20. Intake of selected bioactive compounds from plant food supplements containing fennel (Foeniculum vulgare) among Finnish consumers.

    Science.gov (United States)

    Uusitalo, Liisa; Salmenhaara, Maija; Isoniemi, Merja; Garcia-Alvarez, Alicia; Serra-Majem, Lluís; Ribas-Barba, Lourdes; Finglas, Paul; Plumb, Jenny; Tuominen, Pirkko; Savela, Kirsti

    2016-03-01

    The purpose of this study was to estimate the intake of selected bioactive compounds from fennel-containing plant food supplements (PFS) among Finnish consumers. The estimated average intake of estragole was 0.20mg/d, of trans-anethole 1.15mg/d, of rosmarinic acid 0.09mg/d, of p-coumaric acid 0.0068mg/d, of kaempferol 0.0034mg/d, of luteolin 0.0525μg/d, of quercetin 0.0246mg/d, of matairesinol 0.0066μg/d and of lignans 0.0412μg/d. The intakes of kaempferol, quercetin, luteolin, matairesinol and lignans from PFS were low in comparison with their dietary supply. The intake of estragole was usually moderate, but a heavy consumption of PFS may lead to a high intake of estragole. The intake of trans-anethole did not exceed the acceptable daily intake, but PFS should be taken into account when assessing the total exposure. To our knowledge, this study provided the first intake estimates of trans-anethole, p-coumaric acid and rosmarinic acid in human populations.

  1. Larvicidal activity against Aedes aegypti of Foeniculum vulgare essential oils from Portugal and Cape Verde.

    Science.gov (United States)

    Rocha, Diara Kady; Matosc, Olivia; Novoa, Maria Teresa; Figueiredo, Ana Cristina; Delgado, Manuel; Moiteiro, Cristina

    2015-04-01

    Dengue is a potentially fatal mosquito-borne infection with 50 million cases per year and 2.5 billion people vulnerable to the disease. This major public health problem has recurrent epidemics in Latin America and occurred recently in Cape Verde and Madeira Island. The lack of anti-viral treatment or vaccine makes the control of mosquito vectors a high option to prevent virus transmission. Essential oil (EO) constituents can affect insect's behaviour, being potentially effective in pest control. The present study evaluated the potential use of Foenicultm vulgare (fennel) EO in the control of the dengue vector Aedes aegypti. EOs isolated from fennel aerial parts collected in Cape Verde and from a commercial fennel EO of Portugal were analysed by NMR, GC and GC-MS. trans-Anethole (32 and 30%, respectively), limonene (28 and 18%, respectively) and fenchone (10% in both cases) were the main compounds identified in the EOs isolated from fennel from Cape Verde and Portugal, respectively. The larvicidal activity of the EOs and its major constituents were evaluated, using WHO procedures, against third instar larvae ofAe. aegypti for 24 h. Pure compounds, such as limonene isomers, were also assayed. The lethal concentrations LC50, C90 and LC99 were determined by probit analysis using mortality rates of bioassays. A 99% mortality of Ae. aegypti larvae was estimated at 37.1 and 52.4 µL L-1 of fennel EOs from Cape Verde and Portugal, respectively. Bioassays showed that fennel EOs from both countries displayed strong larvicidal effect against Ae. aegypti, the Cape Verde EO being as active as one of its major constituents, (-)-limonene.

  2. Phytotoxicity and stimulatory impacts of nanosized and bulk titanium dioxide on fennel (Foeniculum vulgare Mill).

    Science.gov (United States)

    Feizi, Hassan; Kamali, Maryam; Jafari, Leila; Rezvani Moghaddam, Parviz

    2013-04-01

    The objective of the this study was to compare concentrations of nanosized TiO2 at 0, 5, 20, 40, 60 and 80 mg L(-1) with bulk TiO2 for phytotoxic and stimulatory effects on fennel seed germination and early growth stage. After 14 d of seed incubation, germination percentage highly improved following exposure to 60 ppm nanosized TiO2. Similar positive effects occurred in terms of shoot dry weight and germination rate. Application of bulk TiO2 particles in 40 ppm concentration greatly decreased shoot biomass up to 50% compared to the control. Application of 40 ppm nanosized TiO2 treatment improved mean germination time by 31.8% in comparison to the untreated control. In addition, low and intermediate concentrations of nanosized TiO2 enhanced indices such as germination value, vigor index and mean daily germination. In general, there was a considerable response by fennel seed to nanosized TiO2 presenting the possibility of a new approach to overcome problems with seed germination in some plant species, particularly medicinal plants.

  3. Bioguided isolation and identification of the nonvolatile antioxidant compounds from fennel (Foeniculum vulgare Mill.) waste.

    Science.gov (United States)

    Parejo, Irene; Viladomat, Francesc; Bastida, Jaume; Schmeda-Hirschmann, Guillermo; Burillo, Jesús; Codina, Carles

    2004-04-07

    A bioguided isolation of an aqueous extract of fennel waste led to the isolation of 12 major phenolic compounds. Liquid chromatography coupled to atmospheric pressure chemical ionization mass spectrometry (LC/UV/APCI-MS) combined with spectroscopic methods (NMR) was used for compound identification. Radical scavenging activity was tested using three methods: DPPH*, superoxide nitro-blue tetrazolium hypoxanthine/xanthine oxidase, and *OH/luminol chemiluminescence. In addition to products described in the literature, eight antioxidant compounds were isolated and identified for the first time in fennel: 3-caffeoylquinic acid, 4-caffeoylquinic acid, 1,5-O-dicaffeoylquinic acid, rosmarinic acid, eriodictyol-7-O-rutinoside, quercetin-3-O-galactoside, kaempferol-3-O-rutinoside, and kaempferol-3-O-glucoside. The structures of eriodictyol-7-O-rutinoside and quercetin-3-O-glucuronide were completely elucidated by two-dimensional NMR experiments. The isolated compounds exhibited a strong antiradical scavenging activity, which may contribute to the interpretation of the pharmacological effects of fennel.

  4. Sage (Salvia officinalis) and fennel (Foeniculum vulgare) improve cryopreserved boar epididymal semen quality study.

    Science.gov (United States)

    Monton, A; Gil, L; Malo, C; Olaciregui, M; Gonzalez, N; de Blas, I

    2015-01-01

    The aim of this study was to evaluate the effect of fennel and sage extracts and the influence of the egg yolk source (fresh or pasteurized) on the success of freezing boar epididymal spermatozoa. In experiment 1, epididymal sperm was recovered by flushing and cryopreserved in a lactose-egg yolk solution supplemented with various concentrations (10, 5 and 2.5 g/L) of sage or fennel. Sperm quality was evaluated (motility, viability, HOST and acrosome integrity) at 0 h and 2 h after thawing. Fennel 10 g/L and sage 5 g/L and control (no extracts) were selected for experiment 2 which also compared fresh or pasteurized egg yolk in the freezing extender and measured DNA integrity of the frozen sperm. Results showed that the interaction between fennel and sage antioxidants with fresh egg yolk significantly improved post thaw sperm quality and protected boar epididymal spermatozoa from cryopreservation damage as a result of oxidative stress.

  5. Antioxidant and anticarcinogenic effects of methanolic extract and volatile oil of fennel seeds (Foeniculum vulgare).

    Science.gov (United States)

    Mohamad, Ragaa Hosny; El-Bastawesy, Amal Mohamad; Abdel-Monem, Mohamad Gamil; Noor, Assmaa Mahmoud; Al-Mehdar, Hussain Abdel Rahman; Sharawy, Sabry Mohamad; El-Merzabani, Mahmuod Mohamad

    2011-09-01

    The present study evaluated the efficacy of fennel seed methanolic extract (FSME) for its antioxidant, cytotoxic, and antitumor activities and for its capacity to serve as a nontoxic radioprotector in Swiss albino mice. We also assessed the natural antioxidant compounds of FSME for use in industrial application. Cytotoxic activity of FSME was evaluated in a mouse model of Ehrlich ascites carcinoma (EAC) and on different types of human cell lines in vitro. The safety and optimum dose of FSME were determined. FSME, 100 mg/kg, was injected intraperitoneally into mice bearing EAC before the mice were exposed to three 2-Gy doses of gamma irradiation. After 30 days, mice were fasted for 18 hours and then sacrificed to observe the lifespan of EAC-bearing hosts. Malondialdehyde (MDA), catalase activity, glutathione content, and total protein in serum, liver tissue, and ascitic fluid were determined. Iron, total iron-binding capacity, transferrin, and ferritin were also evaluated in serum. The data showed the presence of different types of compounds in FSME, such as flavonoids, terpenoids, alkaloids, phenols, and sterols; estragole (71.099%) was the predominant alcohol, gallic acid was the phenolic compound (18.895%), and L-limonene was the most prevalent monoterpene hydrocarbon (11.967%). The mean±standard deviation 50% inhibitory concentrations were 50±0.03 μg/mL for the MCF7 breast cancer cell line and 48±022 μg/mL for the Hepg-2 liver cancer cell line. The significant increase in MDA levels and the significant decrease in catalase activity and glutathione content in liver and tumor tissue in mice bearing EAC were ameliorated after FSME administration. In contrast, total protein content was increased in ascitic fluid. Serum iron was inversely proportional to the levels of ferritin and transferrin and total iron-binding capacity. Administration of FSME before irradiation exerted a cytoprotective effect against gamma irradiation, as manifested by a restoration of the MDA level, catalase activity, and GSH content to near-normal levels. In conclusion, FSME may have remarkable anticancer potential against a breast cancer cell line (MCF7) and liver cancer cell line (Hepg-2). It also showed strong free radical-scavenging activity (100%). Thus, FSME may reduce oxidative stress and protect mouse cells from damage caused by reactive oxygen species. In addition, it could be used as a safe, effective, and easily accessible source of natural antioxidants to improve the oxidative stability of fatty foods during storage. FSME also exhibited an antitumor effect by modulating lipid peroxidation and augmenting the antioxidant defense system in EAC-bearing mice with or without exposure to radiation.

  6. 伞形科杀虫抑菌活性及其活性成分研究进展%Pesticidal Activities and Active Ingredients of Umbelliferea Plants

    Institute of Scientific and Technical Information of China (English)

    吴娇; 张卫云; 周利娟

    2015-01-01

    对伞形科19个属:当归属(Angelica)、芹菜属(Aium)、阿魏属(Ferula)、独活属(Heracleum)、茴香属(Foeniculum)、茴芹属(Pimpinella)、柴胡属(Bupleurum)、蛇床属(Cnidium)、芹属(Prangos)、积雪草属(Centella、毒茴属(Conium)、芫荽属(Coriandrum)、石蛇床属(Petroselinum)、莳萝属(Anethum)、孜然芹属(Cuminum)、葛缕子属(Carum)、蔓芹属(Trachyspermum)、窃衣属(Torilis)和防风属(Saposhnikovia)中具有杀虫抑菌作用植物的生物活性及其活性成分的研究概况按属的顺序进行综述,为进一步将伞形科植物资源应用于植物源农药研究和开发利用提供理论参考.

  7. Aislamiento de consorcios de hongos micorrícicos arbusculares de plantas medicinales y su efecto en el crecimiento de vinca (Catharanthus roseus Isolation of arbuscular mycorrhizal fungi consortia from medicinal plants and their effectiveness on growth of vinca (Catharanthus roseus

    Directory of Open Access Journals (Sweden)

    CLAUDIA DE LA ROSA-MERA

    2012-06-01

    Full Text Available Este trabajo consistió en propagar e identificar hongos micorrícicos arbusculares (HMA recolectados de plantas medicinales (PM de áreas naturales de bosques mixtos, y seleccionar consorcios micorrícicos con base en la promoción del crecimiento de vinca Catharanthus roseus (L G. Don, planta medicinal cuyos alcaloides tienen propiedades antineoplásicas. En la primera fase experimental se recolectaron raíces y suelo rizosférico de 13 PM establecidas en campo para evaluar el porcentaje de colonización total (PCT y cuantificar el número de esporas; además, se tomó una parte del suelo para establecer plantas trampa en invernadero durante 10 meses, y posteriormente evaluar el PCT e identificar los principales géneros de HMA. Todas las PM en su condición natural presentaron colonización micorrícica, observándose cuatro géneros de HMA (Glomus, Acaulospora, Gigaspora y Scutellospora, de los cuales Acaulospora y Glomus fueron los predominantes. En la segunda fase experimental se seleccionaron ocho consorcios con base en el PCT (> 40 % obtenido en las plantas trampa, que correspondieron a las muestras recolectadas de Adiantum capillus-veneris L., Castilleja tenuiflora Benth., Erigeron karvinskianus DC., Pimpinella anisum L., Plantago major L., Ricinus communis L., Rubus fruticosus L. y Rumex mexicanus Meisn. Estos consorcios fueron inoculados en plántulas de C. roseus para evaluar su capacidad de estimular el crecimiento de esta especie en condiciones de invernadero. Después de 70 días, a pesar de presentar un solo género predominante (Glomus, el consorcio aislado de R. mexicanus promovió de manera más consistente el crecimiento de C. roseus (número de hojas, área foliar y peso seco foliar en comparación con el resto de los consorcios micorrícicos.This study consisted on propagating and identifying arbuscular mycorrhizal fungi (AMF collected from medicinal plants (MP of natural areas of mixed forest (Estado de Mexico, and

  8. Antifungal activity and ultrastructural alterations in Pseudocercospora griseola treated with essential oils Atividade antifúngica e alterações ultraestruturais em Pseudocercospora griseola tratado com óleos essenciais

    Directory of Open Access Journals (Sweden)

    Julián Mauricio Ágredo Hoyos

    2012-06-01

    Full Text Available Pseudocercospora griseola, the etiologic agent of angular leaf spot of common bean (Phaseolus vulgaris, is an important disease in all bean-producing regions worldwide and may cause extremely high yield losses. The control of this disease is made more difficult by the pathogen's genetic variability and the inefficiency of fungicides. In this study, of 26 essential oils tested at different concentrations, 25 demonstrated efficiency in affecting the germination of strains 63-31 and 63-63 of the pathogen, reaching inhibition levels of between 80% and 100%. Cymbopogon citratus and Cymbopogon martinii inhibited conidia germination at all concentrations; Eugenia caryophyllata, Cinnamomum sp., Thymus vulgaris, Matricaria recutita, Cordia verbenacea, Origanum vulgare, Cymbopogon nardus, at 0.1 and 0.5%; and Zingiber officinale, Mentha arvensis, Chamaecyparis pisifera, Lavandula officinalis, Ocimum basilicum, Pimpinella anisum, Ocimum selloi, Baccharis dracunculifolia, Laurus nobilis, Citrus sinensis, Melaleuca alternifolia and Eucalyptus globulus, at 0.5%. The main constituents identified were cinnamaldehyde in Cinnamomum sp.; eugenol in E. caryophyllata; trans-β-farnesene in M. recutita; pulegone in C. verbenacea; thymol in T. vulgaris; geranial and neral in C. citratus, and geraniol in C. martini. Through transmission electron microscopy (TEM, it was verified that C. citratus, C. martini and E. caryophyllata presented direct fungitoxic action on P. griseola, causing severe damage to the cellular ultrastructure of the conidia, invalidating germination. These results indicated that essential oils are a promising alternative strategy for the control of angular leaf spot in bean, representing less risk to human health and the environment.Pseudocercospora griseola, agente etiológico da mancha angular do feijoeiro comum (Phaseolus vulgaris, é uma doença importante nas regiões produtoras de feijão em todo o mundo e pode causar perdas de produtividade

  9. Perfil dos consumidores de plantas medicinais e condimentares do município de Pato Branco (PR The profile of consumers medicinal plants and spices of Pato Branco city, Paraná State, Brazil

    Directory of Open Access Journals (Sweden)

    José Abramo Marchese

    2004-06-01

    Full Text Available Diagnosticou-se o perfil dos consumidores de supermercados em Pato Branco (PR, com relação às suas preferências pelas plantas medicinais e condimentares, como subsídio à produção por pequenos produtores rurais. Os consumidores preferem produtos orgânicos, observam a aparência e o preço do produto, e vão ao mercado uma vez por semana, preferencialmente aos sábados. As dez plantas medicinais preferidas, em ordem decrescente, são camomila, anis, boldo, carqueja, guaco, malva, poejo, espinheira-santa, menta e sálvia. As dez plantas condimentares preferidas, em ordem decrescente, são cebola, cravo, canela, orégano, alho, nóz-moscada, pimentão, cebolinha, endro e salsinha.The profile of supermarket users in Pato Branco city, Paraná State, Brazil, was obtained, related to their preference for medicinal and spice plants, as a subsidy to production by small farmers. The consumers prefer organic products observing the appearance and price of the product, and they go once a week to the market, preferably on Saturdays. The ten favorite medicinal plants are Chamomilla recutita, Pimpinella anisum, Vernonia condensata, Baccharis sp., Mikania sp., Malva sp., Cunilla sp., Maytenus sp., Mentha sp. and Salvia officinalis. The ten favorite spices are: Allium cepa, Eugenia caryophyllata, Cinnamomum zeylanicum, Origanum vulgaris, Allium sativum, Myristicia fragans, Capsicum annuum, Allium schoenoprasum, Anethum graveolens and Petroselinum crispum.

  10. Low-angle X-ray scattering from spices

    Energy Technology Data Exchange (ETDEWEB)

    Desouky, O.S. E-mail: omardesouky@yahoo.com; Ashour, Ahmed H.; Abdullah, Mohamed I.; Elshemey, Wael M

    2002-07-01

    Low-angle scattering of X-rays is characterized by the presence of one or more peaks in the forward direction of scattering. These peaks are due to the interference of photons coherently scattered from the molecules of the medium. Thus these patterns are closely linked to the molecular structure of the investigated medium. In this work, low-angle X-ray scattering (LAXS) profiles of five spices; pimpinella anisum (anise), coriandrum sativum (coriander), cuminum cyminum (cumin), foenculum vulgare (fennel) and nigella sativa (nigella or black cumin) are presented after extensive measurements. It is found that all spices exhibit one characteristic peak at a scattering angle around 10 deg. This is equivalent to a value x=0.0565 A{sup -1}, where x=sin({theta}/2)/{lambda}. The full width at half maximum (FWHM) of this peak is found to be characteristic for each type of the investigated spices. The possibility to detect the irradiation of these spices from their LAXS profiles is also examined after 10, 20, 30 and 40 kGy doses of gamma radiation. Except for anise, coriander and cumin at 40 kGy, there are no detectable deviations from the control samples in the scattering profiles of irradiated samples. These results comply with the recommendations of the FDA (US Food and Drug Administration) which defines 30 kGy as the maximum dose for irradiation of spices. The present technique could be used to detect over-irradiation, which causes damage to the molecular structure of some spices.

  11. Antimicrobial activities, pollen diversity and physicochemical properties of natural honey from Southeastern Anatolia of Turkey

    Directory of Open Access Journals (Sweden)

    Menderes Cenet

    2017-02-01

    Full Text Available Background: Honey, a natural sweetener, is produced from the nectar of many plants. The pollen diversity, physicochemical properties, and antimicrobial activities were analyzed in honey samples from Mardin (Southeastern Anatolia. Methods: The melissopalynological method was used to identify and enumerate the pollen granules. Analytical methods and agar well diffusion assays were employed for the determination of some quality parameters and the antimicrobial potential of honey samples, respectively. Results: The pollen composition consisted of 27 taxa belonging to 13 families. The origins of all honey were determined as the multifloral sources. The most predominant taxa were mainly Hedysarum sp., Carduus sp., Melissa officinalis, Gossypium hirsitum, Paliurus spina-christi, Salix sp. and Pimpinella anisum. The secondary pollen taxa were Hedysarum sp., Trifolium sp., Astragalus sp., Salix sp., Paliurus spina-christi, Asphodeline sp., Centaurea sp., Carduus sp., Zea mays and Cistus sp., respectively. Asphodeline sp. as a secondary pollen taxon in a honey sample could be considered as the first report. The pH, total acidity, brix, refractive index, electrical conductivity, moisture and L, a, b values of the samples varied from 3.75 to 4.28, 30 to 42, 67.3 to 85.70, 1.45 to 1.50, 12.40 to 31.61, 0.24 to 0.90, 47.81 to 57.59, -0.94 to 4.31, 20.37 to 31.28, respectively. Antimicrobial activities of the honey specimens were also effective on five bacterial species and two yeast species. Conclusions: Honey samples from Southeastern Anatolia revealed a good diversity of pollen granules. The rich multiflora of honey increases not only its nutritional quality as well as antimicrobial potential on various clinically important microorganisms.

  12. Ethnopharmacy of Turkish-speaking Cypriots in Greater London.

    Science.gov (United States)

    Yöney, Ahmet; Prieto, José M; Lardos, Andreas; Heinrich, Michael

    2010-05-01

    For centuries, in the Eastern Mediterranean region, medicinal plant use has been widely accepted as a treatment method for both minor and major diseases. Although some knowledge exists on the use of such medicinal plants within the Greek Cypriot culture and considerable information is available on various regions in Turkey, no detailed ethnopharmaceutical or ethnobotanical studies exist on Turkish-speaking Cypriots (TSC) both in Cyprus and within one of the largest TSC migrant communities in London, UK. Semi-structured interviews with members of the TSC community in London were conducted by using a questionnaire consisting both of open and closed questions. Open questions were aimed at identifying herbs, spices, medicinal plants and their uses. Also, graded questions were used to define informants' opinions as a quantitative parameter, constructing a statistical basis. A wide range of therapeutic claims were recorded, including 13 chronic illnesses within 85 different plant species, of which 18 were cited more than 10 times. The most frequently mentioned species were Mentha spicata, Salvia fruticosa and Pimpinella anisum. The plants recorded are frequently based on knowledge derived from Turkish-Cypriot traditions, but many examples of medicinal plants with a use based on UK or general western herbal medical traditions were also recorded. Informants highlighted the risk of knowledge loss in younger generations and thus this study serves as a repository of knowledge for use in the future. Due to a lack of knowledge about such usages in the healthcare professions, our study also highlights the need to develop information sources for use by healthcare practitioners in order to raise awareness about benefits and risks of such medical and health food products.

  13. Aniseed oil increases glucose absorption and reduces urine output in the rat.

    Science.gov (United States)

    Kreydiyyeh, Sawsan Ibrahim; Usta, Julnar; Knio, Khuzama; Markossian, Sarine; Dagher, Shawky

    2003-12-19

    Anise (Pimpinella anisum) has been used as a traditional aromatic herb in many drinks and baked foods because of the presence of volatile oils in its fruits commonly known as seeds. Hot water extracts of the seeds have been used also in folk medicine for their diuretic and laxative effect, expectorant and anti-spasmodic action, and their ability to ease intestinal colic and flatulence. The aim of this work was to study the effect of aniseed oil on transport processes through intestinal and renal epithelia and determine its mechanism of action. The essential oils were extracted from the seeds by hydrodistillation and analyzed by gas chromatography. Aniseed oil enhanced significantly glucose absorption from the rat jejunum and increased the Na+-K+ ATPase activity in a jejunal homogenate in a dose dependent manner. The oil, however, exerted no effect on water absorption from the colon and did not alter the activity of the colonic Na+-K+ ATPase. When added to drinking water, it reduced the volume of urine produced in the rat and increased the activity of the renal Na+-K+ ATPase even at extremely low concentrations. It was concluded that aniseed oil increases glucose absorption by increasing the activity of the Na+-K+ ATPase and consequently the sodium gradient needed for the sugar transport. Its anti-diuretic effect is also mediated through a similar mechanism in the kidney whereby a stimulation of the Na+-K+ pump increases tubular sodium reabsorption and osmotic water movement. The colonic Na+-K+ ATPase was however, resistant to the oil.

  14. Biosynthesis of t-anethole in anise: characterization of t-anol/isoeugenol synthase and an O-methyltransferase specific for a C7-C8 propenyl side chain.

    Science.gov (United States)

    Koeduka, Takao; Baiga, Thomas J; Noel, Joseph P; Pichersky, Eran

    2009-01-01

    The phenylpropene t-anethole imparts the characteristic sweet aroma of anise (Pimpinella anisum, family Apiaceae) seeds and leaves. Here we report that the aerial parts of the anise plant accumulate t-anethole as the plant matures, with the highest levels of t-anethole found in fruits. Although the anise plant is covered with trichomes, t-anethole accumulates inside the leaves and not in the trichomes or the epidermal cell layer. We have obtained anise cDNA encoding t-anol/isoeugenol synthase 1 (AIS1), an NADPH-dependent enzyme that can biosynthesize t-anol and isoeugenol (the latter not found in anise) from coumaryl acetate and coniferyl acetate, respectively. In addition, we have obtained a cDNA encoding S-[methyl-14C]adenosyl-l-methionine:t-anol/isoeugenol O-methyltransferase 1 (AIMT1), an enzyme that can convert t-anol or isoeugenol to t-anethole or methylisoeugenol, respectively, via methylation of the para-OH group. The genes encoding AIS1 and AIMT1 were expressed throughout the plant and their transcript levels were highest in developing fruits. The AIS1 protein is 59% identical to petunia (Petunia hybrida) isoeugenol synthase 1 and displays apparent Km values of 145 microm for coumaryl acetate and 230 microm for coniferyl acetate. AIMT1 prefers isoeugenol to t-anol by a factor of 2, with Km values of 19.3 microm for isoeugenol and 54.5 microm for S-[methyl-14C]adenosyl-l-methionine. The AIMT1 protein sequence is approximately 40% identical to basil (Ocimum basilicum) and Clarkia breweri phenylpropene O-methyltransferases, but unlike these enzymes, which do not show large discrimination between substrates with isomeric propenyl side chains, AIMT1 shows a 10-fold preference for t-anol over chavicol and for isoeugenol over eugenol.

  15. Anti- and Pro-Lipase Activity of Selected Medicinal, Herbal and Aquatic Plants, and Structure Elucidation of an Anti-Lipase Compound

    Directory of Open Access Journals (Sweden)

    Muhammad Abubakar Ado

    2013-11-01

    Full Text Available Plants that help in slowing down the digestion of triacylglycerols (TAGs in the pancreas and small intestine of humans play an important role in the reduction of obesity. On the other hand, there may be plants or plant parts that stimulate intestinal lipolytic activity, thus contributing to greater TAG assimilation. The aim of this study was to evaluate the aqueous methanolic extracts of ninety eight (98 medicinal, herbal and aquatic plant materials from Malaysia for their effect on porcine pancreatic lipase (PPL activity and to identify the structure of an anti-lipase compound from one of the sources. The degree of inhibition was also quantified as relative to orlistat activity against PPL (orlistat equivalents. Results revealed that while 19.4% of the extracts were found to have anti-lipase activity ≥80%, 12% were actually found to promote PPL activity. Twenty two percent (22.4% exhibited moderate inhibition (41%–80% and 2% were neutral toward PPL activity. The ripe fruit of Averrhoa carambola and the leaves of Archidendron jiringa (Jack I.C Nielsen L. (jering, Cynometra cauliflora (nam-nam and Aleurites moluccana (L. Willd (candle nut/buah keras had the highest (100% anti-lipase activity and are equivalent to 0.11 µg orlistat/mL. Plants that stimulated lipase activity included Pimpinella anisum L. (aniseed/jintan manis, activating the enzyme by 186.5%. Kaempferol 3-O-rhamnoside was isolated from the ethyl acetate fraction of C. cauliflora leaves and found to be an active lipase inhibitor. The structure was elucidated using 1H-NMR, 13C-NMR and 2D-NMR analyses.

  16. Contaminação fúngica de plantas medicinais utilizadas em chás

    Directory of Open Access Journals (Sweden)

    Ravely Lucena Santos

    2013-04-01

    Full Text Available Este trabalho teve como objetivo avaliar a qualidade microbiológica quanto à presença de fungos filamentosos em ervas do tipo Peumus boldus Molina (Boldo, Pimpinella anisum L. (erva-doce e Matricaria chamomilla L. (Camomila comercializadas na cidade de Campina Grande (Paraíba. Nove amostras de cada planta foram analisadas, totalizando 27 amostras, obtidas aleatoriamente nas feiras livres e barracas informais da cidade. Cada 10 g de amostra foram suspensas em 90 mL de solução salina 0,89% estéril, obtendo-se o concentrado, a partir do qual foram realizadas diluições sucessivas. A seguir, uma alíquota de 0,1mL de cada diluição foi semeada em placas de Petri contendo Ágar Sabouraud Dextrose, as quais foram incubadas à temperatura ambiente por cinco a dez dias. Após este período foram contadas as colônias fúngicas presentes e realizados seus respectivos microcultivos para identificação através de microscópio óptico. Foram encontrados fungos toxigênicos como Aspergillus sp., Penicillium sp. e Fusarium sp., estando o primeiro presente em todas as amostras avaliadas. Também foram identificados os fungos Exophiala sp. e Fonsecaea sp., que possuem importância clínica, podendo causar micose. A forma como estas plantas são armazenadas para comercialização e a falta de fiscalização torna questionável a qualidade das plantas comercializadas para obtenção de chás, o que pode comprometer a saúde do consumidor ao utilizar esses produtos na forma de Chá.

  17. Low-angle X-ray scattering from spices

    Science.gov (United States)

    Desouky, Omar S.; Ashour, Ahmed H.; Abdullah, Mohamed I.; Elshemey, Wael M.

    2002-07-01

    Low-angle scattering of X-rays is characterized by the presence of one or more peaks in the forward direction of scattering. These peaks are due to the interference of photons coherently scattered from the molecules of the medium. Thus these patterns are closely linked to the molecular structure of the investigated medium. In this work, low-angle X-ray scattering (LAXS) profiles of five spices; pimpinella anisum (anise), coriandrum sativum (coriander), cuminum cyminum (cumin), foenculum vulgare (fennel) and nigella sativa (nigella or black cumin) are presented after extensive measurements. It is found that all spices exhibit one characteristic peak at a scattering angle around 10°. This is equivalent to a value x=0.0565 Å -1, where x=sin( θ⧸2)⧸ λ. The full width at half maximum (FWHM) of this peak is found to be characteristic for each type of the investigated spices. The possibility to detect the irradiation of these spices from their LAXS profiles is also examined after 10, 20, 30 and 40 kGy doses of gamma radiation. Except for anise, coriander and cumin at 40 kGy, there are no detectable deviations from the control samples in the scattering profiles of irradiated samples. These results comply with the recommendations of the FDA (US Food and Drug Administration) which defines 30 kGy as the maximum dose for irradiation of spices. The present technique could be used to detect over-irradiation, which causes damage to the molecular structure of some spices.

  18. Anti- and pro-lipase activity of selected medicinal, herbal and aquatic plants, and structure elucidation of an anti-lipase compound.

    Science.gov (United States)

    Ado, Muhammad Abubakar; Abas, Faridah; Mohammed, Abdulkarim Sabo; Ghazali, Hasanah M

    2013-11-26

    Plants that help in slowing down the digestion of triacylglycerols (TAGs) in the pancreas and small intestine of humans play an important role in the reduction of obesity. On the other hand, there may be plants or plant parts that stimulate intestinal lipolytic activity, thus contributing to greater TAG assimilation. The aim of this study was to evaluate the aqueous methanolic extracts of ninety eight (98) medicinal, herbal and aquatic plant materials from Malaysia for their effect on porcine pancreatic lipase (PPL) activity and to identify the structure of an anti-lipase compound from one of the sources. The degree of inhibition was also quantified as relative to orlistat activity against PPL (orlistat equivalents). Results revealed that while 19.4% of the extracts were found to have anti-lipase activity ≥80%, 12% were actually found to promote PPL activity. Twenty two percent (22.4%) exhibited moderate inhibition (41%-80%) and 2% were neutral toward PPL activity. The ripe fruit of Averrhoa carambola and the leaves of Archidendron jiringa (Jack) I.C Nielsen L. (jering), Cynometra cauliflora (nam-nam) and Aleurites moluccana (L.) Willd (candle nut/buah keras) had the highest (100%) anti-lipase activity and are equivalent to 0.11 µg orlistat/mL. Plants that stimulated lipase activity included Pimpinella anisum L. (aniseed/jintan manis), activating the enzyme by 186.5%. Kaempferol 3-O-rhamnoside was isolated from the ethyl acetate fraction of C. cauliflora leaves and found to be an active lipase inhibitor. The structure was elucidated using 1H-NMR, 13C-NMR and 2D-NMR analyses.

  19. Contributions towards a monograph of Phoma (Coelomycetes) III — Supplement. Additional species of section Plenodomus

    NARCIS (Netherlands)

    Boerema, G.H.; Gruyter, de J.

    1999-01-01

    This supplement deals with the diagnostic characteristics and literature data of three species of Phoma sect. Plenodomus: Phoma korfii spec. nov. (synanamorph Sclerotium orobanches Schwein.: Fr.), Phoma pimpinellae spec. nov. (teleomorph Leptosphaeria pimpinellae Lowen & Sivan.) and Phoma etheridgei

  20. Effects of organic and biological fertilizers on fruit yield and essential oil of sweet fennel (Foeniculum vulgare var. dulce)

    Energy Technology Data Exchange (ETDEWEB)

    Moradi, R.; Rezvani Moghaddam, P.; Nasiri Mahallati, M.; Nezhadali, A.

    2011-07-01

    In order to evaluate the effects of different organic and biological fertilizers on quantity and quality of fennel essential oil, an experiment was conducted in a completely randomized block design with three replications. The experimental treatments included two organic (compost and vermicompost) and two biological (Pseudomonas putida and Azotobacter chroococcum) fertilizers, their all twin combinations (Ps. putida + A. chroococcum, Ps. putida + compost, Ps. putida + vermicompost, A. chroococcum + compost, A. chroococcum + vermicompost and compost + vermicompost) and control (non fertilized). There were significant differences between treatments in terms of seed essential oil percentage, essential oil yield; anethole, fenchone, limonene and straggle content in seed essential oil. Results showed that the highest and the lowest percentages of essential oil were obtained in control (2.9%) and A. chroococcum + vermicompost (2.2%) treatments, respectively. The highest essential oil yield (29.9 L ha{sup -}1) and anethole content of essential oil (69.7%) and the lowest contents of fenchone (6.14%), limonene (4.84%) and estragole (2.78%) in essential oil were obtained in compost + vermicompost treatment. It seems that compost + vermicompost treatment compared to other treatments supplied the highest equilibrium of nutrients and water in the root zone of sweet fennel which is led to increasing the anethole content, there upon, decreasing other compounds. Essential oil yield and percentage of anethole content in essential oil were significantly higher in all organic and biological treatments compared with control. (Author) 43 refs.

  1. The effect of foeniculum vulgare essence on production performance, egg quality and reproductive parameters of laying hens

    Directory of Open Access Journals (Sweden)

    Abolghasem Taki

    2014-10-01

    Full Text Available An experiment was conducted to evaluate the effect of fennel essence on performance, egg quality and ovarian morphology of commercial laying eggs for ten weeks. In this experiment, one hundred and forty 30-wk-old commercial egg laying type Hy-Line (W36 were randomly assigned into 4 treatments with 5 replicates and 8 birds per each. Treatments were a control (0.0% essence and diets containing 200, 400 and 600 ppm fennel essence. Performance parameters include egg weight and egg production was recorded daily and feed intake, FCR and egg quality parameters were measured weekly. At the end of the experiment, 2 birds of each replicate was randomly selected and slaughtered for analysis of ovarian morphology. Different levels of fennel essence no significant effect on egg production. Different levels of fennel essence no significant effect on egg production. Levels of 200 and 400 ppm of fennel essence showed a significant increase in egg weight. Levels of fennel essence had no significant effect on feed intake and FCR but it caused significant increase in yolk color and had significant effect on the properties of egg shell. Levels of essence had no significant effect on the relative weight of the ovary, oviduct and stroma but 200 ppm of fennel essence had significant effect on weight of large yellow follicles. The results showed that the addition of fennel essence improved egg weight, egg shell and yolk color and had a significant effect on the large yellow follicles.

  2. Separation and characterization of phenolic compounds in fennel (Foeniculum vulgare) using liquid chromatography-negative electrospray ionization tandem mass spectrometry.

    Science.gov (United States)

    Parejo, Irene; Jauregui, Olga; Sánchez-Rabaneda, Ferran; Viladomat, Francesc; Bastida, Jaume; Codina, Carles

    2004-06-16

    Liquid chromatography (LC) diode array detection (DAD) coupled to negative electrospray ionization (ESI) tandem mass spectrometry (MS/MS) was used for the rapid and sensitive identification of water-soluble phenolic compounds in fennel waste. The plant material was first extracted and then chromatographed on Sephadex LH-20 to afford seven fractions, each of them being subjected to LC-MS analysis. Identification of the compounds was carried out by interpretation of UV, MS, and MS/MS spectra. Forty-two phenolic substances were identified, 27 of which had not previously been reported in fennel, including hydroxycinnamic acid derivatives, flavonoid glycosides, and flavonoid aglycons.

  3. Inhibition of grey mould in vitro and in vivo with essential oil of fennel (Foeniculum vulgare L.

    Directory of Open Access Journals (Sweden)

    Samane MOHAMMADI

    2013-03-01

    Full Text Available The aim of the study was to determine the antifungal effects of the fennel essential oil against fungal pathogen Botrytis cinerea the causal agent of grey mould disease of tomato fruit under in vitro and in vivo conditions. Treatments consisted of five concentrations (0, 200, 400, 600 and 800 lL-1.The fennel oil had a remarkable effect on spore germination of grey mould. The growth of grey mould was completely inhibited by fennel oil at 600 and 800 lL-1.The results in vivo showed that fennel oil increased the shelf life and decreased decay rate of tomato fruits. Also, fennel essential oil positively affected on postharvest quality factors. Treated fruits with fennel oil had significantly higher titrable acidity, total soluble solids, ascorbic acid, and lycopene and -carotene content comparison to control. Thus, these results showed that fennel essential oil has impact on postharvest decay and fruit quality of tomato.

  4. 小茴香种子发芽特性的研究%Study on Germination of Foeniculum Vulgare Seeds

    Institute of Scientific and Technical Information of China (English)

    王羽梅; 任安祥; 潘春香

    2002-01-01

    茴香种子在浸种4小时后吸水就达饱和,最大吸水量相当于风干种子重的185%。茴香种子以浸种16小时、25℃下萌发时发芽率和发芽势最好.相同大小不同饱满程度种子的发芽率和发芽势均无差异,中粒种子的发芽势和发芽率优于大粒和小粒种子.但芽的伸长以大粒种子最快.裂为两半的单果的发芽率、发芽势和芽长均明显好于双悬果.

  5. ANTIMICROBIAL ACTIVITY OF THE EXTRACTS of Foeniculum Vulgare Mill%小茴香的抑菌作用

    Institute of Scientific and Technical Information of China (English)

    高莉; 王丽; 韩阳花; 孙华; 帕提古丽·马合木提

    2004-01-01

    以20%的乙醇溶液提取小茴香中的成分,测定了其提取液对常见食品腐败菌的抑菌效力(抑菌圈直径)及最低抑菌浓度(MIC).其结果为:苏云金杆菌和变形杆菌最敏感,金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌和根霉菌次之,青霉菌和酿酒酵母稍差.

  6. Phytochemical investigation and evaluation of anti-microbial and anti-oxidant activity of Foeniculum vulgare (fennel

    Directory of Open Access Journals (Sweden)

    Shabana Bano

    2016-07-01

    Full Text Available In this study, methanol extract of fennel seeds was examined for its antioxidant and antimicrobial activities. Phytochemical constituents of the fennel extract were also analysed, that revealed the presence of flavonoids, terpenoids and glycosides. Total phenol content of the extract was determined by using Folin-Ciocalteu reagent and was found to be 3.48 ± 4.2 (mg GAE/g DM. The antimicrobial activity of fennel extract was evaluated against following microorganisms: Escherichia coli, Bacillus pumilus, Staphylococcus aureus, Listeria monocytogenes and Enteropathogenic E. coli (EPEC. Maximum antimicrobial activity of the fennel extract was found against Staphylococcus aureus, showing an inhibition zone of 20.00 mm. DPPH radical scavenging assay was used to evaluate the antioxidant activity of the fennel extract and its IC50 value was also calculated. Thus, the results of the present investigation demonstrate antimicrobial and antioxidant activities of fennel extract and also the presence of various phytochemicals.

  7. Antimicrobial activities of essential oil and hexane extract of Florence fennel [Foeniculum vulgare var. azoricum (Mill.) Thell.] against foodborne microorganisms.

    Science.gov (United States)

    Cetin, Bülent; Ozer, Hakan; Cakir, Ahmet; Polat, Taşkin; Dursun, Atilla; Mete, Ebru; Oztürk, Erdoğan; Ekinci, Melek

    2010-02-01

    The objective of this study was to determine the chemical compositions of the essential oil and hexane extract isolated from the inflorescence, leaf stems, and aerial parts of Florence fennel and the antimicrobial activities of the essential oil, hexane extract, and their major component, anethole, against a large variety of foodborne microorganisms. Gas chromatography and gas chromatography-mass spectrometry analysis showed that the essential oils obtained from inflorescence, leaf stems, and whole aerial parts contained (E)-anethole (59.28-71.69%), limonene (8.30-10.73%), apiole (trace to 9.23%), beta-fenchyl acetate (3.02-4.80%), and perillene (2.16-3.29%) as the main components. Likewise, the hexane extract of the plant sample exhibited a similar chemical composition, and it contained (E)-anethole (53.00%), limonene (27.16%), gamma-terpinene (4.09%), and perillene (3.78%). However, the hexane extract also contained less volatile components such as n-hexadecanoic acid (1.62%), methyl palmitate (1.17%), and linoleic acid (1.15%). The in vitro antimicrobial assays showed that the essential oil, anethole, and hexane extract were effective against most of the foodborne pathogenic, saprophytic, probiotic, and mycotoxigenic microorganisms tested. The results of the present study revealed that (E)-anethole, the main component of Florence fennel essential oil, is responsible for the antimicrobial activity and that the essential oils as well as the hexane extract can be used as a food preservative. This study is the first report showing the antimicrobial activities of essential oil and hexane extract of Florence fennel against probiotic bacteria.

  8. Comprehensive Research on Essential Oil and Phenolic Variation in Different Foeniculum vulgare Populations During Transition from Vegetative to Reproductive Stage.

    Science.gov (United States)

    Salami, Maryam; Rahimmalek, Mehdi; Ehtemam, Mohammad Hossein

    2017-02-01

    Chemical composition and antioxidant activity of four fennel populations (England, Spain, Poland and Iran) were investigated during six developmental stages including two vegetative and four reproductive phases. In reproductive phase, the essential oil content of fruits decreased and the maximum content (5.9%) was obtained in immediate fruits. The essential oils were analyzed using GC/MS. trans-Anethole was the main component of the leaves and the fruits oil. In leaves, it ranged from 41.28% in England at late vegetative stage to 56.6% in Poland population at early vegetative stage. Other major compounds of leaves were limonene, α-pinene and (Z)-β-ocimene. In reproductive phases the trans-anethole increased dramatically. It varied from 85.25% in immature fruits from Poland to 90.7% in pre-mature stage from Spain. The highest phenolic content in the extracts at different growth stages obtained 189 mg TAE/g DW at full mature stage of seed in Iran population. The flavonoid of leaves extract ranged from 3 to 7.5 mg QUE/g DW, while in fruits extract varied from 3 to 10.3 mg QUE/g DW. Antioxidant activity of the extracts was evaluated using 1,1-diphenyl-2-picrylhydrazy (DPPH) and β-carotene model systems. Immature and full mature growth stages of fennel population from Spain indicated the highest activity in quenching of DPPH radical (74.2% and 74.5, respectively). Antioxidant activities of the extracts had high positive correlation with their phenolic contents in all fruit maturity stages. Finally, it might probably be suggested that in fennel the hot and humid condition can lead to increase trans-anethole, while the hot and dry one can produce higher amount of phenolics and flavonoids.

  9. Fennel (Foeniculum vulgare) and Fenugreek (Trigonella foenum-graecum) Tea Drinking Suppresses Subjective Short-term Appetite in Overweight Women.

    Science.gov (United States)

    Bae, JiYoung; Kim, JiEun; Choue, Ryowon; Lim, Hyunjung

    2015-07-01

    Appetite controlling has been an main strategy for regulating food intake and energy balance in obesity treatment. The aim of this study was to examine the effects of drinking tea of the medicinal herbs, fennel and fenugreek, on the subjective appetite in overweight Korean women. The study was conducted using a placebo-controlled, single-blinded, randomized, and 3-way crossover design. Nine healthy women were given fennel tea (FT), fenugreek tea (FGT), or placebo tea (PT). After drinking a given tea, a lunch buffet was provided and then food consumption of subjects was analyzed. Subjective appetite, hunger, fullness, desire to eat, and prospective food consumption were measured at seven independent time point using a visual analog scale (VAS). Mean age of 9 subjects were 49.7 ± 4.5 years and their mean body mass index were 24.6 ± 0.6 kg/m(2). There was no significant difference in food consumption in the lunch buffet after drinking each tea; however, with respect to the subjective appetite scale, FGT decreased hunger, led to less prospective food consumption, and increased feelings of fullness compared with the PT (p < 0.05). Similarly, the consumption of FT resulted in decreased hunger, less prospective food consumption, and increased feelings of fullness compared with the PT (p < 0.05). The area under the curve of VAS graph indicated that FGT resulted in a higher feeling of fullness than the PT (p < 0.05). In conclusion, drinking the FT and FGT were significantly effective aid to suppress subjective appetite among overweight women in South Korea.

  10. In vitro antifungal activity and mechanism of essential oil from fennel (Foeniculum vulgare L.) on dermatophyte species.

    Science.gov (United States)

    Zeng, Hong; Chen, Xinping; Liang, Jingnan

    2015-01-01

    Fennel seed essential oil (FSEO) is a plant-derived natural therapeutic against dermatophytes. In this study, the antifungal effects of FSEO were investigated from varied aspects, such as MIC and minimum fungicidal concentration, mycelia growth, spore germination and biomass. The results indicated that FSEO had potent antifungal activities on Trichophyton rubrum ATCC 40051, Trichophyton tonsurans 10-0400, Microsporum gypseum 44693-1 and Trichophyton mentagrophytes 10-0060, which is better than the commonly used antifungal agents fluconazole and amphotericin B. Flow cytometry and transmission electron microscopy experiments suggested that the antifungal mechanism of FSEO was to damage the plasma membrane and intracellular organelles. Further study revealed that it could also inhibit the mitochondrial enzyme activities, such as succinate dehydrogenase, malate dehydrogenase and ATPase. With better antifungal activity than the commonly used antifungal agents and less possibility of inducing drug resistance, FSEO could be used as a potential antidermatophytic agent.

  11. Study the effects of bio and organic fertilizers on growth characteristics and yield of Fennel (Foeniculum vulgar

    Directory of Open Access Journals (Sweden)

    A. Gholami

    2016-05-01

    Full Text Available One of the strategies for improving the quality of agricultural products is using bio-fertilizers. So in order to study the effects of mycorrhizal fungi, vermicompost and humic acid on growth characteristics and yield of Fennel, an experiment was studied on research farm of agricultural college of Shahrood University. This study was conducted as factorial experiment based on completely randomized block design with three replications. Factors include: mycorrhizal fugi( inoculated with Glomus intraradices and without inoculated, vermicompost(0,4,8 ton.ha-1 and humic acid( foliar and non-foliar application. Results of this study showed, the main effects of mycorrhizal fungi, vermicompost and humic acid on height, no. of umbels, seed weight per plant, biological and grain yield were significant. Mycorrhizal inoculation increased biological and grain yield compared with control plots about 8.5 and 20%, respectively. In this study 1000 seed weight significantly was affected by vermicompost and humic acid application. Interaction effects of mycorrhizal fungi, vermicompost on no. of seed per umbel, 1000 seed weight and grain yield was significant. So , the highest 1000 seed weight and grain yield were obtained from combination of mycorrhizal inoculation and 8 ton vermicompost( about 21 and 45 % increase in compare with control. Also the effects of combination of mycorrhizal fungi, vermicompost and humic acid on plant height and biological yield of fennel were significant.

  12. Antonio Lamarra, Roberto Palaia, Pietro Pimpinella: Le prime traduzioni della Monadologie di Leibniz (1720-1721), Florenz : Olschki, 2001 (Lessico intellectuale Europeo ; 85) (Rezension)

    OpenAIRE

    2014-01-01

    Dankbar nimmt man nun aus den Händen italienischer Forscher eine vergleichende begriffsgeschichtliche Untersuchung über die ersten Übersetzungen der Monadologie von Leibniz in die Hand. Deren Übersetzung las das Publikum nämlich früher als das Original, das 1714 entstand. Diese Schrift bestimmte - neben der 1710 veröffentlichten Theodizee - die frühe Wirkung von Leibniz, allerdings eben nicht in der originalen (französischen) Form, denn diese wurde erst 1840 in einer Leibniz-Ausgabe des Hegel...

  13. Examining food additives and spices for their anti-oxidant ability to counteract oxidative damage due to chronic exposure to free radicals from environmental pollutants

    Science.gov (United States)

    Martinez, Raul A., III

    was found to be 12.6 quercetin equivalents/g. The Maximum free radical-scavenging action and free radical reducing power of coriander seed extract was observed at a concentration of 50microg galic acid equivalents (GAE) (Sharma Om P. et al 2009) Anise - Pimpinella Anisum - the seeds of anise were investigated and the extracts showed marked antioxidant activity, both linoleic acid and liposome model systems. The antioxidant activity of alcohol extracts decreased in the order chamomile flowers > gtill seeds>gtnise seeds. The extract also showed good free radical-scavenging 2,2'-diphenyl-l-picrylhydrazyl (DPPH) activity, indicating that they act as hydrogen donors. (Sharma, Om P. et al 2009) Carrot Seed-- Daucus Carota --carrot seed is known for containing potent amounts of antioxidants. It is known for its phenomenal free-radical scavenging and oxygen radical absorbing abilities. It contains high levels of carotenoids and components of vitamin A and powerful antioxidants. It also has high levels of tocopherol, a fat soluble form of vitamin E Carrot seed is a natural antioxidant. Total Phenolic content in extracts was measured by the Folin-Ciocalteu (FC) reagent method. Results were expressed as micrograms of galic acid equivalents (GAE). Celery Seed -- Apium Graveolens - Celery contains well known antioxidants like vitamin C and flavonoids. Scientists have identified at least a dozen other types of antioxidants in celery. Celery is an important food source of conventional antioxidant nutrients, including vitamin C, beta-carotene, and manganese. But its claim to fame may very well be its phytonutrients. Many of these phytonutrients fall into the category of phenolic antioxidants and have been shown to provide anti-inflammatory benefits as well. Celery is an excellent source of vitamin K and molybdenum. It is a very good source of folate, potassium, dietary fiber, manganese, and pantothenic acid. Celery is also a good source of vitamin B2, copper, vitamin C, vitamin A

  14. Survey of insect fauna from plants medicinal, aromatic and seasoning and disinfestation by the process of radiation; Levantamento da entomofauna de plantas medicinais, aromaticas e condimentares e desinfestacao pelo processo de irradiacao

    Energy Technology Data Exchange (ETDEWEB)

    Reis, Fabricio Caldeira

    2013-07-01

    The present study aimed to survey the insect fauna associated with medicinal plants, aromatic dehydrated and seasoning trade in Sao Paulo city, using different doses of gamma radiation with the aim of disinfestation of the material and determine the lethal dose of gamma radiation on Sphaericus gibboides. From April to May 2011 were collected in 10 establishments the following sample materials: Melissa officinalis L. (Lemongrass), Mentha piperita L. (Mint), Ocimum basilicum L. (Basil), Origanum vulgare L. (Oregano), Rosmarinus officinalis L. (Rosemary), Thymus vulgaris L. (Thyme), Senna alexandrina Mill (senna), Coriandrum sativum L. (Coriander), Petroselinum crispum (Mill.) Fuss (salsa) and Pimpinella anisum L. (Fennel), Baccharis trimera (Less.) DC. (Gorse), Chamomilla recutita L. (= M. recutita L.) (chamomile), Laurus nobilis L. (Blonde) (Lauraceae); Capsicum annuum L. (Sweet paprika), Bixa orellana L. (Spice) (Bixaceae) and Peumus boldus Molina (Boldo). The first screening showed that all the tested materials did not show the presence of adult insects. After 45 days 940 adult insects were found and larvae from eggs. The substrates analyzed Chamomilla recutita showed the highest rate of infestation, with 70,6%. Pelmus boldus, Laurus nobilis, Chamomilla recutita and Capsicum annuum, had the highest species diversity. Baccharis trimera, Bixa orellana, Melissa officinalis, Origanum vulgare and Coriandrum sativum showed no infestation. The species was Lasioderma serricorne the insect with the largest number of individuals found (936), higher percentage of infestation in different materials (62.5%) and lots, and highest occurrence (68,75%) materials (M. piperita, S. alexandrian, P. anisum, Chamomilla recutita, P. crispum, L. nobilis, C. sativum, C. annuum, O. basilicum, P. boldus and T. vulgaris). The following materials were selected for testing disinfestation by irradiation process: Bixa orellana, Capsicum annuum, Cassia angustifolia, Coriandrum sativum, Mentha

  15. Evaluation of the control ability of five essential oils against Aspergillus section Nigri growth and ochratoxin A accumulation in peanut meal extract agar conditioned at different water activities levels.

    Science.gov (United States)

    Passone, María A; Girardi, Natalia S; Etcheverry, Miriam

    2012-10-15

    Essential oils (EOs) from boldo [Pëumus boldus Mol.], poleo [Lippia turbinata var. integrifolia (Griseb.)], clove [Syzygium aromaticum L.], anise [Pimpinella anisum] and thyme [Thymus vulgaris]) obtained by hydrodistillation were evaluated for their effectiveness against the growth of Aspergillus niger aggregate and A. carbonarius and accumulation of ochratoxin A (OTA). The evaluation was performed by compound dissolution at the doses of 0, 500, 1500 and 2500μL/L in peanut meal extract agar (PMEA) and exposure to volatiles of boldo, poleo (0, 1000, 2000 and 3000μL/L) and clove oils (0, 1000, 3000 and 5000μL/L), taking into account the levels of the water activity of the medium (a(W) 0.98, 0.95, 0.93). Statistical analyses on growth of Aspergillus strains indicated that the major effect was produced by oil concentrations followed by substrate a(W), and that reductions in antifungal efficiency of the oils tested were observed in vapor exposure assay. At all a(W) levels, complete fungal growth inhibition was achieved with boldo EO at doses of 1500 and 2000μL/L by contact and volatile assays, respectively. Contact exposure by poleo and clove EOs showed total fungal inhibition at the middle level tested of 1500μL/L, regardless of a(W), while their antifungal effects in headspace volatile assay were closely dependent on medium a(W). The fumigant activity of poleo (2000μL/L) and clove oils (3000μL/L) inhibited growth rate by 66.0% and 80.6% at a(W) 0.98 and 0.93, respectively. OTA accumulation was closely dependent on a(W) conditions. The antiochratoxigenic property of the volatile fractions of boldo, poleo and clove EOs (1000μL/L) was more significant at low a(W) levels, inhibition percentages were estimated at 14.7, 41.7 and 78.5% at a(W) 0.98, 0.95 and 0.93, respectively. Our results suggest that boldo, poleo and clove oils affect the OTA biosynthesis pathway of both Aspergillus species. This finding leaves open the possibility of their use by vapor exposure

  16. Uso regular de plantas medicinais para fins terapêuticos em famílias residentes na zona rural de Santo Antônio de Jesus – Bahia – Brasil

    Directory of Open Access Journals (Sweden)

    Raíssa Silva Santos

    2017-10-01

    Full Text Available Introdução: O uso de plantas medicinais é uma prática comum de cuidado primário nas populações em geral. Foi introduzido no Brasil, por influência das culturas indígena, africana e europeia e caracteriza-se por ser oriundo do conhecimento e da tradição popular. Objetivos: Sendo o Recôncavo da Bahia uma região de forte influência africana, o objetivo deste estudo foi investigar o uso das principais plantas medicinais e identificar seu uso pela população de um de seus municípios, valorizando o acervo cultural e o saber popular de seus moradores quanto aos benefícios que essas plantas trazem para a população. Metodologia: Para a obtenção dos dados, foi aplicado um questionário sociodemográfico, contendo questões sobre o uso de plantas para fins medicinais em chás ou xaropes com 154 entrevistados. Resultados: Desse universo de pessoas, 74,71% tinham o costume de usar remédios caseiros e 60,0% eram do gênero feminino. Os vegetais mais citados para fins medicinais foram: Lippia alba (n=71, Cymbopogon citratus (n=31, Pimpinella anisum (n=30, Peumus boldus (n=14, Plantago major e Ocimum basilicum (n=13 cada; sendo utilizados principalmente como calmante, dores em geral e contra gases. Quanto ao local de obtenção dos vegetais, os entrevistados cultivavam a planta (94,6%, pegavam do cultivo de amigos e vizinhos (1,8%, compravam na feira livre (1,8%, compravam em farmácia ou adquiriam de outros lugares (0,9%. Conclusões: Os dados evidenciam a relevância da prática do uso de plantas para manutenção da saúde dos moradores do município pesquisado, sendo parte integrante de seu contexto sociocultural. As diferentes finalidades do uso de plantas para fins medicinais mostram que mais estudos são necessários para avaliar as propriedades dessas plantas e seus efeitos no organismo humano.

  17. Self-therapy practices among university students in Palestine: focus on herbal remedies.

    Science.gov (United States)

    Sawalha, Ansam F; Sweileh, Waleed M; Zyoud, Sa'ed H; Jabi, Samah W

    2008-12-01

    Herbal self-therapy is a common practice among Palestinians. However, no published data are available on herbal self-therapy in the Middle East in general, and in Palestine in particular. This study was conducted to (1) determine the extent of herbal self-therapy among university students, (2) investigate the different types of herbal remedies used and (3) investigate the correlates and reasons associated with such practices. This cross-sectional, descriptive study was carried out using a structured questionnaire that contained five sections: (1) demographics; (2) medication knowledge and self-care orientation; (3) types of herbal remedies used; (4) clinical conditions treated; and finally, (5) the reasons reported by students for herbal self-therapy practice. Pearson chi(2), multiple logistic regression and one-way ANOVA were performed using SPSS 13 program. 33.9% of the respondents reported using herbal remedies in self-therapy. Female gender, students at medical colleges and those with high self-care orientation were significant predictive model for herbal use. Sage (Salvia fruticosa L.), chamomile (Chamaemelum nobile L.), anise (Pimpinella anisum L.), and thyme (Thymus vulgaris L.) were the most commonly utilized herbal remedies. The types of herbal remedies selected were significantly influenced by gender, but not by the level of medication knowledge or self-care orientation. Herbal remedies were used primarily for the treatment of headache, flu, menstrual pain and sore throat. The main motivating factor for using herbal remedies reported for using herbal remedies was simplicity of symptoms. Herbal self-therapy was a common practice among university students. Health care providers need to be aware of the students' self-therapy practices and need to have sufficient knowledge regarding herbs not simply because of the widespread use, but also because of significant reported side effects. Academics need to consider offering courses about herbal remedies to

  18. Distribution of Trans-Anethole and Estragole in Fennel (Foeniculum vulgare Mill of Callus Induced from Different Seedling Parts and Fruits

    Directory of Open Access Journals (Sweden)

    Abd El-Moneim Mohamed Radwan AFIFY

    2011-03-01

    Full Text Available In the present study, seeds from local cultivar of fennel were germinated on Murashige and Skoog medium (MS without plant growth regulators. Different types of explants from the growing seedling such as cotyledonal leaves, hypocotyls, epicotyls and roots were cultured on MS medium, contained different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D either alone or with kinetin. Differential responses in the essential oil constituents were observed in the induction and development of callus. The major components of essential oils includes estragole, trans-anethole, limonene and fenchone were studied under different conditions to find out the best methods which could be used to reduce the amount of estragole (not favorite for human consumption and increase the amount of trans-anethole.

  19. Comparison of the effect of Foeniculum vulgare and St John’s wort (Hypericum perforatum on the climacteric symptoms and sexual activity in menopausal woman

    Directory of Open Access Journals (Sweden)

    Khadijeh Abdali

    2016-01-01

    Full Text Available Background and objective: Menopause is the permanent ceasing of menstruation for one year and in this period, women potentially suffer from chronic signs and symptoms resulted from estrogen deficiency. With respect to heavy responsibility of women in the society, providing their health is highly required by health system. The objective of the current research is the comparison between the effects of Fennel and Hypiran on severity of climacteric symptoms and sexual performance in menopausal women. Methodology: In a blind placebo-controlled clinical trial, 120 menopausal women of 45-60 of age in Shiraz were randomly categorized in three groups after a checkup for general health. The first group was given the daily dose of three 160mg tablets of Hypiran, the second group was given the daily dose of three 30mg tablets of Fennel and the third group was given three placebo all for the period of 8 weeks. The data was collected by filling out questionnaire for female sexual function and Greene climacteric scale in three phases (before treatment, 4weeks after treatment and 8 weeks after treatment. Thus, 33 individuals in Hypiran group, 33 individuals in Fennel group and 32 individuals in placebo group cooperated until the end of the research. The collected data was analyzed by SPSS software (version 20. Findings: The average age of the participants was 50/52. The score of climacteric symptoms was 42/12±5/1 in Hypiran group before treatment and it decreased to 30/42±4/77 after treatment. The score of climacteric symptoms was 37/81±7/2 in Fennel group before treatment and it decreased to 24/93±6/3 after treatment and regardless of the time, there was a significant difference between the three groups (P≤0.001. The score of sexual function was 18/2±2/61 in Hypiran group before treatment and it increased to 22/78±2/61 after treatment. The score of sexual function was 18/38±2/29 in Fennel group before treatment and it increased to 22/51±2/25 after treatment and there was a significant difference in the mean score of sexual function between the three groups (P≤0.001. Conclusion: Fennel and Hypiran are effective in improvement of menopausal signs and symptoms in women suffering from climacteric symptoms. Also they have positive effect on sexual function of menopausal women.

  20. Effect of Foeniculum vulgare (fennel) vaginal cream on vaginal atrophy in postmenopausal women: A double-blind randomized placebo-controlled trial.

    Science.gov (United States)

    Yaralizadeh, Masomeh; Abedi, Parvin; Najar, Shahnaz; Namjoyan, Foroogh; Saki, Amal

    2016-02-01

    Vaginal atrophy is one of the main concerns of postmenopausal women. The aim of the present study was to investigate the effect of fennel vaginal cream on vaginal atrophy in postmenopausal women in Ahvaz, Iran. This double-blind randomized controlled trial was conducted on 60 postmenopausal women (45-65 years of age) in Ahvaz, Iran. The study participants were randomly divided into one of two groups, receiving either a placebo (n=30) or fennel 5% vaginal cream (n=30) administered as one application per day (5g/day) for 8 weeks. The vaginal pH and maturation vaginal index (MVI) were measured at baseline and 8 weeks after the intervention, while the vaginal atrophy symptoms was measured at baseline and at 2, 4, and 8 weeks after the intervention. The data were analyzed using the independent t-test, chi-square test, paired sample t-test, and the generalized estimating equation. The number of superficial cells increased significantly in the fennel group after 8 weeks compared to the control group (76.1±15.3 vs. 11.8±8, peffective means to manage the symptoms of vaginal atrophy in postmenopausal women and is devoid of side effects. Larger studies are necessary to confirm the positive impact of fennel for vaginal conditions occurring among postmenopausal women. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  1. Study of Ningxia Haiyuan Foeniculum vulgare Mill.cytological characteristics%宁夏海原小茴香细胞学特性研究

    Institute of Scientific and Technical Information of China (English)

    雷茜; 张欣; 贝盏临

    2011-01-01

    以宁夏海原小茴香为材料,跟踪观察细胞的分裂过程,并对其染色体进行核型分析.结果表明,宁夏海原小茴香细胞的染色体数目为2n=2x=22,染色体平均总长度为272.55+1.08 μm,最长染色体为5.58 μm,最短染色体为1.48 μm,最长染色体与最短染色体之比为3.78;染色体平均长度为4.55+1.79 μm,长臂平均长度为2.67+0.90 μm,短臂平均长度为1.90+0.92.μm,平均臂比为1.52±0.29,臂比介于1.01-1.70之间,最长染色体臂与最短染色体臂之比为2.02;其核型公式为K(2n)=22=20 m(2SAT)+2 sm,具有1对随体;染色体相对长度组成为2n=2s+8M1+10M2+2L,属于“2B”对称型核型.

  2. 小茴香果实中总黄酮含量测定方法研究%Study on the Total Flavonoids Contents in Foeniculum vulgare Mill

    Institute of Scientific and Technical Information of China (English)

    古丽巴哈尔·阿巴拜克力

    2009-01-01

    目的:测定小茴香果实中的总黄酮含量,以利于后续的提取操作.方法:采用分光光度法,以芦丁为标准样品,测定小茴香中的总黄酮含量;通过单因素和正交试验确定果实中总黄酮的最佳提取条件.结果:总黄酮的最佳提取条件为:乙醇浓度70%,提取时间2 h,料液比1:30,提取温度70℃,在此条件下提取率可达2.871.结论:试验结果可能为以后小茴香资源的开发利用提供重要的理论依据.

  3. Inhibitory effect of different fennel (Foeniculum vulgare) samples and their phenolic compounds on formation of advanced glycation products and comparison of antimicrobial and antioxidant activities.

    Science.gov (United States)

    Salami, Maryam; Rahimmalek, Mehdi; Ehtemam, Mohammad Hossein

    2016-12-15

    In this study, antioxidant, antibacterial and antiglycation properties of methanolic extracts of 23 fennel samples were evaluated and their major compounds were determined using HPLC analysis. The anti-glycative activity of extracts was evaluated in the bovine serum albumin (BSA)/glucose system. The level of glycation, conformational alterations and protein binding to RAGE receptors were assessed by Congo red binding assay and a brown staining method. Among samples, Kh1 from Iran possessed the highest TFC (14.8mgQUEg(-1)), TPC (262mg/g DW) and antioxidant activity (IC50=76μg/ml). The HPLC results revealed high variation in 23 fennel samples according to their major flavonoid (quercetin, apigenin and rutin) and phenolic (chlorogenic, caffeic and 1,5-dicaffeoylquinic acid) compounds. The antibacterial activity of methanolic extracts against four food-borne pathogens was also assessed. The seed extracts of Kh1 and En samples showed moderate to good inhibitory activities (MICs=62.5-125μg/ml) against three bacteria, as well as high anti-glycative activity.

  4. Chemical composition, antimicrobial and antioxidant activities of anethole-rich oil from leaves of selected varieties of fennel [Foeniculum vulgare Mill. ssp. vulgare var. azoricum (Mill.) Thell].

    Science.gov (United States)

    Senatore, Felice; Oliviero, Filomena; Scandolera, Elia; Taglialatela-Scafati, Orazio; Roscigno, Graziana; Zaccardelli, Massimo; De Falco, Enrica

    2013-10-01

    The chemical composition and biological activity of the essential oils obtained from the leaves of two different cultivars of Florence fennel cropped under three different fertilization treatments (Control not fertilized; Mineral Fertilization; Compost from Municipal Solid Wastes) have been analyzed. All the oils were characterized by high anethole concentration and some showed also a good percentage of limonene. Thus, the leaves of Florence fennel, which are agricultural wastes, could be used for the recovery of anethole to be used for its flavoring and biomedical properties. The antimicrobial activity expressed by assays on the examined oils indicates an appreciable effect, generally higher on Gram-positive bacteria. The various samples of Florence fennel analyzed did not show any results with FRAP test. The DPPH test showed a weak capacity of the samples to catch the free radicals from the solution, attributable to their content in anethole.

  5. Antioxidant activities of phenolics, flavonoids and vitamin C in two cultivars of fennel (Foeniculum vulgare Mill. in responses to organic and bio-organic fertilizers

    Directory of Open Access Journals (Sweden)

    Zeinab A. Salama

    2015-01-01

    Full Text Available Field experiment was conducted to study the effect of organic and bio-organic fertilizers on dry weight; yield, total phenolics (TPC, total flavonoids (TFC, vitamin C and on their antioxidant activities of two sweet fennel cultivars Dolce and Zefa fino. Results strongly showed that there were significant differences between sweet fennel cultivars. Generally the highest values of all parameters were obtained when fennel plants were supplemented with 50% NPK + 50% organic fertilizer and bio fertilizer when compared with control treatment. The highest values of TPC, TFC and Vit. C were recorded by Zefa fino cultivar when received 50% NPK + 50% organic treatment. The antioxidant activities of both cultivars were evaluated and Dolce cultivar showed the highest DPPH·− scavenging activity expressed as IC50 compared with Zefa fino cultivar. In addition, Dolce cultivars exhibited the highest value for Fe2+-chelating activities for organic and bio-organic fertilizers followed by Zefa fino when compared to control treatment. Dolce cultivar generally showed superiority than Zefa fino in all measured parameters.

  6. 小茴香对小鼠免疫功能的影响%Effect of Foeniculum vulgare Mill on the Immunological Functions in Mice

    Institute of Scientific and Technical Information of China (English)

    董华泽; 王艳苹; 袁新松; 王丽平

    2009-01-01

    [目的]研究小茴香对环磷酰胺模型小鼠免疫功能的影响.[方法]以环磷酰胺制备小鼠免疫低下模型并结合体外药理试验,测定灌胃给药后小鼠体内巨噬细胞吞噬率、血清溶血素抗体水平,并观察植物血凝素诱导T淋巴细胞的增殖反应.采用含药血清体外培养小鼠腹腔巨噬细胞,MTT法考察巨噬细胞的活性,并观察体外培养的巨噬细胞吞噬红细胞的吞噬百分率和吞噬指数.[结果]小茴香提高免疫抑制小鼠碳粒廓清率,促进血清溶血素形成以及促进T淋巴细胞增殖.[结论]小茴香有提高小鼠免疫功能的作用.

  7. 正交法优选盐制小茴香工艺%Study on Processing Technology of Foeniculum vulgare Mill with Orthogonal Experimental Design

    Institute of Scientific and Technical Information of China (English)

    林楠; 杜中梅; 贾天柱

    2009-01-01

    [目的]优选小茴香的最佳炮制工艺.[方法]以反式茴香醚和水溶性浸出物的含量为评价指标,选择加盐量、炒制度及炒制间为考察因素,采用正交设计L9(34),优选小茴香的最佳炮制工艺.[结果]小茴香的最佳炮制工艺为:小茴香每20g加15mL盐水(含0.4g盐),闷润1.5h,在110~120℃下炒4min.[结论]盐制小茴香的最佳炮制工艺合理可行.

  8. Characteristics of fatty acids and essential oil from sweet fennel (Foeniculum vulgare Mill. var. dulce) and bitter fennel fruits (F. vulgare Mill. var. vulgare) growing in Turkey.

    Science.gov (United States)

    Coşge, Belgin; Kiralan, Mustafa; Gürbüz, Bilal

    2008-01-01

    Oil content in sweet and bitter fennels was obtained 12.22% and 14.41%, respectively. The C(18:1 c6), C(18:2), C(18:1 c9) and C(16:0) acids corresponding to approximately 97% of total oil was recorded as principal fatty acids. The ratios of essential oil from sweet and bitter fennels were found similar (average 3.00%). trans-Anethole, estragole and fenchone were found to be the main constituents in both fennels. The compound with the highest value in the two oil samples was trans-anethole as 95.25% (sweet) and 75.13% (bitter). While estragole was found in bitter fennel oil in a remarkable amount (15.51%), sweet fennel oil contained small amounts of estragole (2.87%). Fenchone was found <1% in sweet and approximately 5% in bitter fennel. p-Anisaldehyde in bitter fennel essential oil, and alpha-pinene and gamma-terpinene in sweet fennel essential oil were not recorded, and these compounds were found very low or <1%.

  9. 小茴香嫩茎的组织培养%Study on Tissue Culture of Foeniculum vulgare Mill. Fresh Stem

    Institute of Scientific and Technical Information of China (English)

    帕提曼·阿不都热合曼; 秦勇; 玉苏甫·阿不力提甫

    2007-01-01

    以小茴香嫩茎为外植体,进行了组织培养研究.结果表明,适合诱导小茴香愈伤组织的最佳培养基为改良MS培养基+6-BA 1.5 mg/L+NAA0.5 mg/L,诱导愈伤组织所需的时间最短,诱导率也高,形成的愈伤组织颜色浓绿,生长速度适中,质地致密;最佳生根培养基为改良MS培养基+6-BA0.5 mg/L+NAA 1.0 mg/L,生根率高,生根数多,质量好.

  10. 茴香复合调味酱的研制%Study on the Mixed-condiment of Foeniculum Vulgare Mill

    Institute of Scientific and Technical Information of China (English)

    孙秀菊

    2010-01-01

    茴香不仅具有丰富的营养,而且具有特殊香味,是一种芳香类调料.将新鲜的茴香用盐腌,绞碎,浓缩,添加调味料之后制成复合调味酱.通过对腌制时间,离心时间,CMC含量以及调味品四个因素的调控,利用正交试验的直观分析,从而得到最佳组合的茴香复合调味酱.

  11. Toxic effects of sub-chronic exposure of male albino rats to emamectin benzoate and possible ameliorative role of Foeniculum vulgare essential oil.

    Science.gov (United States)

    El-Sheikh, El-Sayed A; Galal, Azza A A

    2015-05-01

    Emamectin benzoate (EB) is an avermectin insecticide used extensively in pest control on vegetable and field crops. Few studies have been done for evaluating adverse effects of EB. In the current study, we evaluated the toxic effects of EB on male rats and the possible ameliorative role of fennel essential oil (FEO). Thirty two male rats were randomly divided into 4 equal groups. All groups were treated orally with distilled water (control group), 0.5mlFEOkg(-1) BW (FEO group), 2.5mgEBkg(-1) BW (EB group), and 0.5mlFEOkg(-1) BW+2.5mgEBkg(-1) BW (FEO+EB group) for 28 days. The obtained results showed that EB treatment resulted in a significant decrease in body weight, body weight gain, RBC count, Hb concentration, % PCV, MCV and MCHC. Moreover, EB significantly decreased total leukocyte, lymphocyte, monocyte and platelet count but significantly increased granulocyte count. EB markedly decreased total protein, albumin, globulin, IgG and IgM concentrations with a significant increase in TNF-α secretion. EB had a negative impact on the liver as it significantly increased ALT, ALP, and MDA, while decreasing SOD activity. Regarding to the histopathological examination, EB treatment induced coagulative necrosis and blood vessels congestion of the liver in treated rats. Furthermore, it resulted in depletion and necrosis of the white pulp of the spleen in treated rats. The co-administration of FEO with EB, however, improved the majority of parameters studied, suggesting that FEO is an important substance in decreasing toxic effects of EB.

  12. Evaluate of Some Germination and Emergence Characteristics of Fennel (foeniculum vulgare Mill Producted from Seeds under Application Organic and Biological Fertilizers

    Directory of Open Access Journals (Sweden)

    M.B Amiri

    2013-04-01

    Full Text Available In order to study the effects of manure and biofertilizers application that were applied on parent plants, on the germination and emergence indices of produced seeds of fennel, two laboratory and greenhouse experiments based on completely randomized design with four replications were conducted in Ferdowsi University of Mashhad, Iran. Treatments were consisted of 11 different seed types that were produced on parent plants fed by: Compost (C, Vermicompost (V, Azotobacter (A, Pseudomonas (P, A+C, C+P, C+V, P+A, P+V, V+A and Control. Results of greenhouse study revealed that most studied treatments were effective on the height of fennel. Moreover, V and C+P treatments were superior in terms of emergence percentage and aerial dry weight, respectively. Results of laboratory experiment showed that C+V treatment were superior to other treatments in terms of germination percentage, germination rate, germination index and mean germination time. Overall, the experimental findings revealed that seed producing by manure and biofertilizers utilization on parent plants is a suitable method for production of organic seeds that have optimal germination and early seedling growth indices.

  13. Study on anatomical structure of stalks and roots of Inner Mongolia Foeniculum vulgare and "Youhui No.1" Foeniculum vulgare%内蒙古茴香与油茴1号根、茎解剖结构的比较

    Institute of Scientific and Technical Information of China (English)

    潘春香; 何金明; 仲兆清; 庞琢; 庞云; 冯艳红

    2010-01-01

    应用石蜡包裹法探讨内蒙古茴香与油茴1号的根、茎的解剖结构差异.结果表明:茴香的根和茎均由表皮、皮层、中柱组成,根发育到后期出现了木栓层、木栓形成层和栓内层;茎发育到一定程度,在皮层里形成了精油的分泌腔,且分泌腔的数量随着植株的发育呈增多的趋势.高精油含量品种"油茴1号"的根和茎各组织的细胞大小、导管数量、分泌腔数量及直径均比内蒙古茴香的大而多.为茴香解剖学研究及品种鉴定提供了解剖学依据.

  14. Capacity of earwig Marava arachidis (Yersin to access fennel plants Foeniculum vulgare Mill in laboratory and field Capacidade da tesourinha Marava arachidis (Yersin em acessar a planta de erva-doce Foeniculum vulgare Mill no laboratório e no campo

    Directory of Open Access Journals (Sweden)

    Charles Ira Abramson

    2007-12-01

    Full Text Available This research was aimed at obtaining data about earwig behavior (Marava arachidis Y. on fennel agro-ecosystems and evaluating its potential capacity to access plants in the absence of aphids under laboratory and field conditions. This study establishes a baseline to evaluate earwigs as biological controls to combat aphids that attack fennel plants. Two experiments were developed. In experiment 1, earwigs were studied under laboratory conditions, in experiment 2 under field conditions. Independent variables were sex, exposure to the essential oil of fennel for 24 or 48 hours, and whether the fennel plant was vegetative or flowering. The results indicated that earwigs will climb a fennel plant in the absence of aphids and that few statistical significant results were obtained among the independent variables examined. A difference between male and females was noted in the field experiment in animals receiving 48 h of exposure to the essential oil of fennel. The terminal height reached by males and females in the 48 h vegetative and flower condition also differed.Este trabalho objetivou obter os primeiros dados a respeito da tesourinha (Marava arachidis Y. em agroecossistema de erva-doce, além de avaliar sua capacidade potencial em acessar essas plantas na ausência de afídeos, em laboratório e campo, permitindo futuros trabalhos visando a seu uso no controle biológico para combater os afídeos que atacam essa cultura. Assim, dois experimentos foram realizados, sendo que no experimento 1 as tesourinhas foram estudadas em condições de laboratório e no segundo experimento em condições de campo. As variáveis independentes observadas foram: sexo, exposição a óleo essencial de erva-doce por 24 e 48 horas, em plantas no estágio vegetativo e reprodutivo. Os resultados indicaram que as tesourinhas acessam as plantas na ausência de afídeos e que existem poucas diferenças entre as variáveis estudadas. A diferença entre os machos e as fêmeas foi observada em campo quando os insetos foram expostos por 48 horas a óleo essencial de erva-doce. A altura máxima atingida pelos machos e pelas fêmeas, quando expostos ao óleo essencial de erva-doce por 48 horas nos estágios vegetativos e de florescimento, também diferiram.

  15. Napa River Salt Marsh Restoration Project. Volume 1: Environmental Impact Statement

    Science.gov (United States)

    2004-06-01

    Foeniculum vulgare) can invade higher elevations of this community, especially along the bases of levees. Iceplant (Carpobrotus edulis) is one of the...sweet fennel ( Foeniculum vulgare), and prickly lettuce. Urban Urban vegetation is dominated by landscaped grasses, flowers, shrubs, and trees, both

  16. Environmental Impact Statement for the New San Clemente Project, Monterey County, California - Regulatory Permit Application Number 16516S09. Appendices.

    Science.gov (United States)

    1987-09-01

    include: Artemisla dourlaslana (Mugwort), Baeharls pi.uraris (Coyote Bush), Rubus vitifollus (Blackberry), Foeniculum vul-are (Sweet Fennel...Baccharis pilularis), blackberry, mule ft (Saccharis viminea), and sweet(fennel ( Foeniculum vulgare). The most extensive stands of this habitat type occur in

  17. NaCl胁迫对茴香植株生长及生理指标的影响%Effect of NaCl stress on growth and physiological indexes in fennel (Foeniculum vulgare Mill.) seedling

    Institute of Scientific and Technical Information of China (English)

    韩凤叶; 肖艳辉; 潘春香; 庞琢; 施胜坚

    2010-01-01

    利用营养液培养的方式,研究了NaCl胁迫对茴香植株生长、可溶性糖、脯氨酸以及丙二醛含量的影响.结果表明:随着NaCl浓度的增加,茴香植株的各项生长指标均呈下降趋势;经NaCl胁迫后,可溶性糖含量在120mmol·L-1NaCl处理下,达到最大,并与对照差异达显著水平,茴香中脯氨酸含量比对照有所增加,而丙二醛含量呈先降低再增加的趋势.

  18. 超临界流体萃取小茴香及其提取物在卷烟中的应用%Extraction from Foeniculum Vulgare Mill. With Supercritical Fluid and its Application in Cigarette

    Institute of Scientific and Technical Information of China (English)

    何书杰; 张高峰; 崔韬; 郭刚

    2009-01-01

    本文采用超临界流体技术对小茴香进行了萃取,考察了不同萃取条件下萃取物的性质,用GC-MS对提取物主要化学成分进行了鉴定,与溶剂浸提法所得的结果进行了对比.并进行了卷烟加香加料试验,旨在探索天然烟用香料的加工方法.

  19. 小茴香挥发油化学成分及抑菌作用的研究%Studies on chemical compositions and antibacterial activity of volatile oil of foeniculum vulgare mill

    Institute of Scientific and Technical Information of China (English)

    高莉; 斯拉甫·艾白; 韩阳花

    2007-01-01

    通过GC/MS联用分析了小茴香挥油化学成分和各组分的相对含量,分离出17个峰,鉴定出了13个化合物,占所测挥发油总量的96.5%.其中主要成分是反式茴香脑(81.81%),α-水芹烯(4.15%)、爱草脑(3.52%)等.同时,对其抑茵作用做了研究,结果表明所选4种菌种的生长均明显受到了抑制,其中,对金黄色葡萄球茼抑制作用最强,其次是枯草芽孢杆茵和变形杆菌,大肠杆菌最差.研究结果还发现,样品用乙醚稀释后能加强其抑茵作用.

  20. 小茴香萃取物中脂肪酸成分的GC-MS分析%Analysis of fatty acids in Foeniculum vulgare Mill. seeds by gas chromatography-mass spectrometry

    Institute of Scientific and Technical Information of China (English)

    杨天林; 张抒峰; 杨敏丽; 吴茂江

    2000-01-01

    对宁夏固原种植的小茴香籽油用正己烷进行了回流提取,萃取物经皂化和甲酯化反应处理后,利用气相色谱-质谱(GC-MS)进行分析,鉴定了产物中的脂肪酸组分.结果表明,17种脂肪酸中,含量最高的是十八碳烯-5-酸(78.562%),其次是十八碳二烯酸(8.352%)、棕榈酸(7.259%)和硬脂酸(2.103%).

  1. Influence of treatment of Foeniculum vulgare with multi salt on sprout and growth%复合盐处理对茴香发芽及幼苗生长的影响

    Institute of Scientific and Technical Information of China (English)

    任安祥; 王羽梅

    2007-01-01

    在发芽期间用60 mmol/L的复合盐对茴香种子进行处理,出苗后再分别用30 mmol/L、60 mmol/L、90 mmol/L的复合盐进行幼苗处理,观测了复合盐对种子发芽和幼苗生长的影响.研究结果表明:种子发芽期用复合盐处理可以明显提高种子的发芽率和发芽势;盐处理组在出苗期前的干物质质量下降速度比未处理组快,而出苗后的干物质量比未处理组高.幼苗期用30 mmol/L、60 mmol/L的盐处理对幼苗的生长有促进作用,而用90 mmol/L的盐处理对幼苗生长有抑制作用;种子发芽期盐处理后再进行幼苗期盐处理,对幼苗的生长比单独处理种子或单独处理幼苗效果都好.

  2. Study on Foeniculum Vulgare Mill as Penetration Enhancer for the Permeation of 5-Fluorouracil%小茴香对5-氟脲嘧啶的促渗作用研究

    Institute of Scientific and Technical Information of China (English)

    沈琦; 徐莲英

    2001-01-01

    目的:研究小茴香提取物及主要成分茴香醛、茴香脑等对5-氟脲嘧啶(5-Fu)的促渗作用.方法:采用体外透皮实验方法,用Valia-Chien水平扩散池为实验装置,测定模型药物5-氟脲嘧啶的累积渗透量及渗透系数、增渗倍数,确定其促渗效果的强弱.结果:完成了小茴香所含成分小茴香油、茴香脑、茴香醛等对5-氟脲嘧啶的促渗实验,上述药物对5-氟脲嘧啶均有一定的促渗作用,增渗倍数分别为7.14、4.17、9.54.结论:小茴香油、茴香脑、茴香醛等对5-氟脲嘧啶具有一定的促渗作用.

  3. Composition and Antimicrobial Activity of Essential Oil from Foeniculum vulgare Mill. Seeds%小茴香籽精油成分及其抗菌活性研究

    Institute of Scientific and Technical Information of China (English)

    钟瑞敏; 肖仔君; 张振明; 刘健南; 王羽梅

    2007-01-01

    对小茴香籽精油的化学成分及其抗菌活性进行了研究.采用GC-MS技术结合Kovats保留指数(KI)对比法较好地鉴定了该精油的化学成分.小茴香籽精油的主体成分为反式茴香脑(70.78%)、爱草脑(7.78%)、小茴香酮(5.87%)、柠檬烯(5.85%)和δ-3- 蒈烯(2.01%). 对受试的7种食源性致病菌和2种腐败性真菌,小茴香籽精油表现出优良的广谱性抗菌活性,其中黑曲霉和副溶血性嗜盐菌对该精油最为敏感,最小抑菌量(MIC)分别小于 0.004% 和 0.015%(体积分数).

  4. 高速逆流色谱分离小茴香中香气成分研究%Separation of Flavors from Foeniculum Vulgare by High-speed Counter-current Chromatography

    Institute of Scientific and Technical Information of China (English)

    肖阳; 谢建春; 马琳贺; 马洁峰

    2007-01-01

    以小茴香为原料,采用水中直接蒸馏的方法提取小茴香精油.采用V(正己烷):V(乙酸乙酯):V(甲醇):V(水)=5:2:5:2为溶剂体系,上相为固定相,下相做流动相,高速逆流色谱(HSCCC)转速854.6 r/min,流动相流速2 mL/min,UV检测波长254 nm,在保留时间95~100 min,分离出茴香醛,茴香脑,茴香酮一组化合物,结构经GC-MS确认.

  5. 维药小茴香根皮化学成分初步研究%Preliminary study on the chemical components of the roots of Foeniculum vulgare Mill

    Institute of Scientific and Technical Information of China (English)

    麦迪乃·赛福丁; 美丽万·阿布都热依木

    2012-01-01

    目的 对维药小茴香根皮化学成分进行初步研究.方法 采用试管法和滤纸法对维药小茴香根皮的水、酸水、95%乙醇、石油醚提取物进行研究,通过预实验,初步推断维药小茴香根皮中可能合有的化学成分.结果 维药小茴香根皮中可能含有糖类及其苷、内酯化合物、酚类、鞣质、黄酮类、生物碱、挥发油、油脂、三萜及甾醇等化学成分.结论 本研究为维药小茴香根皮的进一步开发利用提供了实验基础.

  6. 阴离子对球茎茴香生长和精油含量的影响%The Effects of Anion on Plant Growth and Quantity of Essential Oil in Foeniculum vulgare

    Institute of Scientific and Technical Information of China (English)

    王羽梅; 任安祥; 潘春香; 高野泰吉

    2002-01-01

    分别用60 mmol·L-1浓度的HCO-3、Cl-、SO2-4复合盐处理定植后的球茎茴香,结果Cl-对生长的影响不明显,HCO-3和SO2-4处理的生长略差些,但植株中Na+、Ca2+、Mg2+含量均增加,全氮、全磷和K+的含量则都下降.精油含量以Cl-处理的为最高,SO2-4的次之,HCO-3的无变化.Cl-和SO2-4的精油含量增加主要是苧烯的增加.

  7. Effect of Formula Fertilization on the Growth and Yield of Foeniculum Vulgare Mill%配方施肥对小茴香生长和产量的影响

    Institute of Scientific and Technical Information of China (English)

    秦梦; 张益铭; 杨兴栋; 尹春梅

    2014-01-01

    通过施加不同肥料和施肥量,研究配方施肥对小茴香生长和产量的影响.以小茴香的施肥比例,微肥种类,N、P、K施用总量3个因素,每个因素设计3个水平,按照正交试验L9(33)表进行试验.以产量作为指标,综合考虑,选出小茴香的最佳施肥方案.优选出的小茴香最佳施肥方案为:施肥比例为2∶1∶2,微肥种类为Mn0.3%,N、P、K施用总量为150 kg/hm2.试验优选了小茴香的最佳施肥方案,为小茴香的进一步研究提供参考.

  8. 紫外分光光度法测定小茴香不同器官中总黄酮%Determination of the Total Flavonoids in Different Organs of Foeniculum vulgare Mill. by UV Spectrophotometry

    Institute of Scientific and Technical Information of China (English)

    朱金霞; 郭生虎; 郑国保

    2010-01-01

    采用紫外分光光度法测定小茴香不同器官提取物中总黄酮的含量.结果表明:小茴香不同器官中总黄酮含量在38.4~76.8 μg/mL范围内与吸光度呈良好的线性关系,加标回收率在96.7%~99.22%范围内,相对标准偏差为1.01%;该方法简便、准确、重现性好,可用于总黄酮的含量测定.

  9. 民族药小茴香不同炮制品中各元素的光谱分析%Spectral Analysis of Elements in Different Samples of Processed Foeniculum vulgare

    Institute of Scientific and Technical Information of China (English)

    张帆; 李臻; 哈木拉提·吾甫尔; 田树革

    2010-01-01

    中药材经过不同方法炮制后将改变其中物质的组成和含量,从而影响药理活性.利用电感耦合等离子体发射光谱法(ICP-AES),原子吸收光谱法(AAS)和原子荧光光谱法(AFS)全面扫描并分析民族药小茴香生品及其9种不同炮制品中与人体健康和生命相关的常量元素,微量元素和重金属的种类及含量.结果显示,3种光谱分析方法均能快速准确地分析中药材中各种元素含量及其变化趋势,从小茴香中共检测出32种元素,与生品相比,不同炮制方法使某些元素发生了明显不同的变化,表明炮制方法与元素变化有较强的相关性,说明辅料不仅能改变元素含量而且能与有效成分一道发挥协同治疗疾病,并旨次发现炮制将导致苇金属Hg的含量急剧增高,临床使用清炒小茴香时存在重大安全隐患.实验拓宽了光谱法在中药安全性评价中的应用.

  10. Most Common Herbal Medicines in the Treatment of Iranian Children: A Systematic Review

    OpenAIRE

    Masoumeh Ghazanfarpour; Ramin Sadeghi; Mohammad Ali Kiani; Imaneh Khorsand; Masumeh Saeidi

    2014-01-01

    Introduction: To assess the efficacy of foeniculum Vulgar, menthe longifolia and Garlic in Iranian children. Methods and Matherials: Nine databases such as MEDLINE, Scopus and the Cochrane Central Register of Controlled Trials as well as domestic  database (Persian) such as SID, Iranmedex, Magiran, Medlib, Irandoc, and Google Scholar were searched using keywords such as  “child” and “complementary treatments or alternative treatments or herbal treatments or Anthum Foeniculum or Capillaceum or...

  11. Most Common Herbal Medicines in the Treatment of Iranian Children: A Systematic Review

    Directory of Open Access Journals (Sweden)

    Masoumeh Ghazanfarpour

    2014-12-01

    Full Text Available Introduction: To assess the efficacy of foeniculum Vulgar, menthe longifolia and Garlic in Iranian children. Methods and Matherials: Nine databases such as MEDLINE, Scopus and the Cochrane Central Register of Controlled Trials as well as domestic  database (Persian such as SID, Iranmedex, Magiran, Medlib, Irandoc, and Google Scholar were searched using keywords such as  “child” and “complementary treatments or alternative treatments or herbal treatments or Anthum Foeniculum or Capillaceum or Foeniculum Officinale or Foeniculum vulgare or menthe longifolia or Garlic in June 2014 Result: Five studies were included in the systematic review. Our systematic review showed beneficial effect of Foeniculum Vulgare on redacting of infant colic and also led to significant increase on prolactin levels in lactating mothers.  Base on only study, comparison between two groups (Shirafza and placebo Drops did not show any significant difference in regarding infant weight gain. Menthe longifolia combined with ORS improved frequency of defecation, Volume and consistency of stool. Also Review Systematic showed that Garlic significantly decreased fever, frequency and duration of diarrhea, leukocyte in stool.   Conclusion: herbals medicine (foeniculum Vulgar, menthe longifolia and Garlic had beneficial effect on Women's serum prolactin levels, infantile colic, frequency of defecation, Volume, consistency of stool. However, this result should be interpreted with caution which low number of sample and methodological quality.

  12. Study on Volatiles in Foeniculum Vulgare Mill by Gas Chromatrography-mass Spectrometry Combining with Simultaneous Distillation and Solvent Extraction%采用同时蒸馏萃取-气相色谱/质谱分析小茴香的挥发性成分

    Institute of Scientific and Technical Information of China (English)

    谢建春; 孙宝国; 郑福平; 刘玉平; 崔奕

    2004-01-01

    通过比较精油收率及精油气相色谱分析含有的组分数量,确定同时蒸馏萃取方法提取小茴香挥发性成分较佳条件为: 乙醚萃取剂,小茴香∶乙醚∶水为10 g∶40 mL∶100 mL,提取时间3h.气质联机分析所提取的挥发油, 鉴定出反式-茴香脑(74.56%)、爱草脑(2.40%)、葑酮(1.05%)、茴香醛(3.51%)、1,8-桉树脑(0.15%)、樟脑(0.05%)、萜烯类(2.97%)、香豆素 (0.03%)、十八碳烯酸 (0.18%)等43种化合物,总相对百分含量为89.03%.

  13. 超临界CO2流体萃取法提取小茴香挥发油化学成分的研究%Study of Chemical Constituents of the Essential Oil from Foeniculum Vulgare Mill. by Supercritical Fluid Extraction

    Institute of Scientific and Technical Information of China (English)

    张莉; 王晓华; 杨天祝

    2007-01-01

    采用超临界CO2流体萃取法(SFE)从小茴香中提取挥发油.用气相色谱-质谱联用技术对化学成分进行分析,共鉴定了50种成分,占挥发油总成分的81%以上.超临界CO2流体萃取法提取的挥发油能更真实、全面地反映小茴香中的化学成分.

  14. The influence of the foeniculum vulgare mill on cytokine in hepatic fibrosis rats%小茴香对实验性肝纤维化大鼠细胞因子结缔组织生长因子(CTGF)的影响

    Institute of Scientific and Technical Information of China (English)

    刘玉平; 许晏; 甘子明

    2009-01-01

    和模型组有统计学差异(P<0.05).模型组和治疗组比较均有统计学差异(P<0.05).小茴香高剂量治疗组降低细胞分泌CTGF的作用高于小茴香中、低剂量治疗组和秋水仙碱治疗组(P<0.05).结论: 小茴香低、中、高剂量治疗组均有降低CTGF的分泌、抑制大鼠肝脏炎症、改善肝脏纤维化的作用.

  15. Analysis of the Volatile Constituents from Foeniculum Vulgare Mill by Gas Chromatography-Mass Spectrometry Coupled with Solid Phase Microextraction%固相微萃取/气相色谱-质谱法分析小茴香挥发性成分

    Institute of Scientific and Technical Information of China (English)

    谢建春; 孙宝国; 郑福平; 刘玉平; 刘丽

    2005-01-01

    考察了萃取样品温度、萃取纤维吸附时间及解吸时间对于固相微萃取的影响,确定了固相微萃取小茴香挥发性成分较佳的实验条件为:萃取样品温度60 ℃,萃取纤维吸附时间40 min,脱附温度250 ℃,脱附时间5 min.用计算机谱库检索结合气相色谱保留指数二维定性方法,从小茴香的固相微萃取/气相色谱-质谱联用分析谱图中,鉴定出反式-茴香脑、顺式-茴香脑、爱草脑、葑酮、p-茴香醛、1,8-桉树脑、樟脑等35种小茴香挥发性成分,总质量分数98.70%.

  16. ANTIMICROBIAL POTENTIAL OF VOLATILE OIL ISOLATED FROM SOME TRADITIONAL INDIAN SPICES

    Directory of Open Access Journals (Sweden)

    Bhuyan Anupam

    2012-04-01

    Full Text Available In the present study the antimicrobial activity of Volatile Oils isolated from Traditional Indian Spices, Anethum Graveolens (Umbelliferae, Foeniculum Vulgare (Umbelliferae and Coriandrum Sativum (Umbelliferae were studied. The isolated Volatile Oils in varying concentrations were studied against Staphylococcus Coagulase, E.Coli, Streptococcus fecaelis and Staphylococcus aureus, by paper disc diffusion method, using Amoxicillin as standard drug.The results indicated that all the Volatile Oil samples from Anethum Graveolens (Dill, Foeniculum Vulgare (Fennel and Coriandrum Sativum (Coriender has antimicrobial potential and were active against almost all the microorganisms but in a dose dependent manner. Foeniculum Vulgare by far was the most potent volatile oil showing the highest activity against Staphylococcus Coagulase.

  17. Chemical Composition, Antimicrobial and Antioxidant Activities of Essential Oils from Organically Cultivated Fennel Cultivars

    Directory of Open Access Journals (Sweden)

    Mahmoud A. Saleh

    2011-02-01

    Full Text Available Essential oils of the fruits of three organically grown cultivars of Egyptian fennel (Foeniculum vulgare var. azoricum, Foeniculum vulgare var. dulce and Foeniculum vulgare var. vulgare were examined for their chemical constituents, antimicrobial and antioxidant activities. Gas chromatography/mass spectrometry analysis of the essential oils revealed the presence of 18 major monoterpenoids in all three cultivars but their percentage in each oil were greatly different. trans-Anethole, estragole, fenchone and limonene were highly abundant in all of the examined oils. Antioxidant activities of the essential oils were evaluated using the DPPH radical scavenging, lipid peroxidation and metal chelating assays. Essential oils from the azoricum and dulce cultivars were more effective antioxidants than that from the vulgare cultivar. Antimicrobial activities of each oil were measured against two species of fungi, two species of Gram negative and two species of Gram positive bacteria. All three cultivars showed similar antimicrobial activity.

  18. First record and redescription of Binodoxys brevicornis (Hymenoptera: Braconidae: Aphidiinae from Argentina

    Directory of Open Access Journals (Sweden)

    Leticia ZUMOFFEN

    2013-01-01

    Full Text Available Binodoxys brevicornis (Haliday se cita por primera vez en Argentina. Este afidiino se encontró parasitando al pulgón Hyadaphis foeniculi (Passerini en Foeniculum vulgare (Miller, una especie vegetal de crecimiento espontáneo, comúnmente asociada a los bordes de cultivos de alfalfa. Binodoxys brevicornis se redescribe e ilustra.

  19. Bazı bitkisel kökenli uçucu yağların Tetranychus cinnabarinus (Boisduval, 1867) (Acari: Tetranychidae) üzerine kontakt ve repellent etkileri

    OpenAIRE

    TOPUZ, Emine; MADANLAR, Nilgün

    2010-01-01

    In this study, contact and repellent effects of 5 different plant essential oils against Tetranychus cinnabarinus (Boisduval, 1867) (Acari: Tetranychidae) were investigated under laboratory conditions. Essential oils of aerial parts, leaves or seeds of Mentha pulegium Linnaeus (Labiatae), Foeniculum vulgare Miller (Umbellifera), Pistacia terebinthus Linnaeus (Anacardiaceae), Schinus molle Linnaeus (Anacardiaceae) and Vitex agnus-castus Linnaeus (Verbenaceae) plants, grown around Antalya regio...

  20. Interspecific Associations between Cycloneda sanguinea and Two Aphid Species (Aphis gossypii and Hyadaphis foeniculi) in Sole-Crop and Fennel-Cotton Intercropping Systems

    OpenAIRE

    Francisco S Fernandes; Ramalho, Francisco S.; Malaquias,José B.; Godoy, Wesley A. C.; Santos, Bárbara Davis B.

    2015-01-01

    Aphids cause significant damage to crop plants. Studies regarding predator-prey relationships in fennel (Foeniculum vulgare Mill.) and cotton (Gossypium hirsutum L.) crops are important for understanding essential ecological interactions in the context of intercropping and for establishing pest management programs for aphids. This study evaluated the association among Hyadaphis foeniculi (Passerini) (Hemiptera: Aphididae), Aphis gossypii Glover (Hemiptera: Aphididae) and Cycloneda sanguinea (...

  1. Cottage cheeses functionalized with fennel and chamomile extracts: comparative performance between free and microencapsulated forms

    OpenAIRE

    Caleja, Cristina; Ribeiro, Andreia; BARROS, LILLIAN; Barreira, João C. M.; Antonio, Amilcar L.; Oliveira, M.B.P.P.; Barreiro, M.F; Ferreira, Isabel C. F. R.

    2016-01-01

    Globally, there is a trend for healthy food products, preferably incorporating natural bioactive ingredients, replacing synthetic additives. From previous screening studies, extracts of Foeniculum vulgare Mill. (fennel) and Matricaria recutita L. (chamomile) maintained nutritional properties and improved the antioxidant activity of cottage cheese. Nevertheless, this effect was limited to 7 days. Accordingly, aqueous extracts of these plants were microencapsulated in alginate and incorporated ...

  2. Essential oil of some seasonal flowering plants grown in Saudi Arabia

    Directory of Open Access Journals (Sweden)

    S.A. Al-Mazroa

    2015-03-01

    Full Text Available The constituents of the essential oils of Rumex vesicarius, Erucaria hispanica, Schimpera arabica, Savignya parviflora, Horwoodia dicksoniae, Sisymbrium irio, Plantago amplexicaulis, Plantago boissieri, Arnebia linearifolia, Foeniculum vulgare, Trigonella hamosa, Lotus halophilus, Reseda muricata, Cenchrus ciliaris is reported. These oils were analyzed by GC/MS and most of them are being studied for the first time.

  3. Installation Restoration Program. Phase I: Records Search. Sunnyvale Air Force Station, California

    Science.gov (United States)

    1985-07-01

    bindweed (Convolvulus arvensis), 3-27 bristly ox tongue (Picris echioides), and sweet fennel ( Foeniculum vulgare ) (U.S. Army Corps of Engineers, 1981...glaucus), common barley (Hordeum vulgare ), and common foxtail (Hordeum hystrix). Introduced forbe common onsite are black mustard (Brassica nigra

  4. Ocorrência de Binodoxys brevicornis (Haliday, 1833 (Hymenoptera, Braconidae, Aphidiinae no Brasil Occurrence of Binodoxys brevicornis (Haliday, 1833 (Hymenoptera, Braconidae, Aphidiinae in Brazil

    Directory of Open Access Journals (Sweden)

    Marcus V. Sampaio

    2003-12-01

    Full Text Available Specimens of the aphid parasitoid, Binodoxys brevicornis (Haliday, 1833, were reared from the mummies of Cavariella aegopodii (Scopoli, 1763 collected on Foeniculum vulgare (Apiaceae at the Campus of the Universidade Federal de Lavras, in the city of Lavras, Minas Gerais State on September/2002. This is the first record of B. brevicornis in Brazil.

  5. Effect of essential oils on Aspergillus spore germination, growth and mycotoxin production: a potential source of botanical food preservative

    OpenAIRE

    Negero Gemeda; Yimtubezinash Woldeamanuel; Daniel Asrat; Asfaw Debella

    2014-01-01

    Objective: To investigate effect of essential oils on Aspergillus spore germination, growth and mycotoxin production. Method: In vitro antifungal and antiaflatoxigenic activity of essential oils was carried out using poisoned food techniques, spore germination assay, agar dilution assay, and aflatoxin arresting assay on toxigenic strains of Aspergillus species. Results: Cymbopogon martinii, Foeniculum vulgare and Trachyspermum ammi (T. ammi) essential oils were tested against toxicogeni...

  6. تأثیر تنش خشکی بر عملکرد و اجزای عملکرد اکوتیپ های مختلف رازیانه (Foeniculum vulgare L.

    Directory of Open Access Journals (Sweden)

    یوسف احمدی خاندانقلی

    2017-04-01

    Full Text Available به‌منظور بررسی تغییرات عملکرد اکوتیپ­های مختلف گیاه دارویی رازیانه تحت تأثیر تنش خشکی، آزمایشی مزرعه­ای در سال 1392 به‌صورت کرت­های خردشده در قالب بلوک­های کامل تصادفی در سه تکرار در مزرعه تحقیقاتی پردیس ابوریحان دانشگاه تهران واقع در پاکدشت اجرا گردید. عامل اصلی (کرت­های اصلی شامل 3 سطح آبیاری 80، 140 و 180 میلی‌متر تبخیر از تشتک تبخیر کلاس A، به ترتیب به‌عنوان شاهد (آبیاری نرمال، تنش متعادل و تنش شدید بود. عامل فرعی (کرت­های فرعی را 51 اکوتیپ رازیانه تشکیل داد. در این بررسی نتایج نشان داد که تنش متعادل باعث افزایش مقدار اسانس در دانه می­شود. همچنین نتایج بیانگر معنی­دار بودن اثر سطوح مختلف آبیاری بر وزن خشک کل اندام هوایی (در سطح احتمال 5 درصد، عملکرد دانه، وزن هزار دانه و مقدار اسانس (در سطح احتمال یک درصد گردید. مقایسه میانگین­ها نشان داد که بیشترین عملکرد دانه در تیمار آبیاری نرمال به دست آمد. مقایسه میانگین اکوتیپ­ها نشان داد که بیشترین عملکرد دانه مربوط به اکوتیپ الموت و کمترین مقدار مربوط به اکوتیپ­های تفرش و یزد بود. مقایسه میانگین اثرات آبیاری بر صفت مقدار اسانس نیز نشان داد که بیشترین مقدار اسانس در دانه مربوط به تیمار تنش متعادل است. اثرات متقابل آبیاری در اکوتیپ برای صفت وزن خشک کل اندام هوایی در سطح پنج درصد و برای صفت مقدار اسانس در سطح یک درصد معنی­دار بود. همچنین مقایسه میانگین اثرات متقابل نشان داد که بیشترین مقدار اسانس مربوط به اکوتیپ مشکین‌شهر در تنش متعادل بود.

  7. Phytoactivity of secondary compounds in aromatic plants by volatile and water-soluble ways of release

    OpenAIRE

    A. S. Dias; Dias, L. S.

    2005-01-01

    Phytoactivity should be expected as a generalized trait of secondary plant compounds if their primary role is defence against co-occurring plants, and volatilization should be their predominant way of release in dry climates while in wet climates water leaching should prevail. Bioassays were designed to compare the ability of volatiles and water-solubles of four aromatic species thriving in dry environments (Cistus salvifolius L., Foeniculum vulgare Miller, Myrtus communis L., and Rosmarinus ...

  8. AROMATIC PLANT OF LAMIACEAE FAMILY FOR USE IN PHYTOTHERAPY

    OpenAIRE

    L. A. Logvinenko; L. A. Khlypenko; N. V. Marko

    2016-01-01

    Introduction. Therapeutic action of terpenoids of essential oil in uncommon plants like Elsholtzia stauntonii, Agastache foenic-ulum, Monarda fistulosa, Perovskia atri-plicifolia, Majorana hortensis, therefore the study for these plants application in phyto-therapy is timely. The study for componen-tial content of essential oil of 18 uncommon species from Lamiaceae family from the collection of Nikitsky Botanical Garden to increase therapeutic possibilities and their practical use in phytothe...

  9. Final Programmatic Environmental Assessment: Demolition and Abandonment of Atlas and Titan Facilities Vandenberg Air Force Base, California

    Science.gov (United States)

    2005-09-13

    ensure compliance with 40 CFR 61.14O, National Emission Standard for Asbestos and 29 CFR 1926.58, Asbestos Construction Standards. AFI 32-1053 Pest ...Management Program 1-Apr-1999 Provides guidance for pest management programs at Air Force installations. It also establishes a program to ensure...Surrounding vegetation mowed dominated by Foeniculum vulgare, Brassica nigra, Plantago coronopus, Avena barbata, and Melotis indicus. Survey for birds in

  10. 小茴香的优质高产栽培

    Institute of Scientific and Technical Information of China (English)

    易思荣; 李娟; 全健

    2009-01-01

    @@ 小茴香别名小茴、谷茴、香丝菜等,为伞形科植物茴香(Foeniculum vulgare.)的干燥成熟果实,具有祛寒止痛、理气和胃之功能,用于胃寒胀痛、少腹冷痛、痛经、疝痛、食少呕吐等症.

  11. 小茴香的优质高产栽培

    Institute of Scientific and Technical Information of China (English)

    易思荣; 李娟; 全健

    2009-01-01

    小茴香别名小茴、谷茴、香丝菜等,为伞形科植物茴香(Foeniculum vulgare Mill.)的干燥成熟果实,具有祛寒止痛、理气和胃之功能,用于胃寒胀痛、少腹冷痛、痛经、疝痛、食少呕吐等症。

  12. Application of Hyperspectal Techniques to Monitoring & Management of Invasive Plant Species Infestation

    Science.gov (United States)

    2008-01-09

    Foeniculum vulgare) –an aggressive invader into grasslands of the Coastal Sage Scrub and mixed chaparral semiarid shrublands of southern California ... Chaparral . 5.1.4. Extending Mapping Beyond the Image Space Using CART Modeling 5.1.4.1. Introduction As shown in previous sections remote sensing...contrast in canopy between invasive blue gum trees (Eucalyptus globulus) and the surrounding shrublands in California chaparral have proved useful for

  13. Flora of vascular plants of selected Poznań cemeteries

    Directory of Open Access Journals (Sweden)

    Aneta Czarna

    2012-12-01

    Full Text Available The presence of 395 species of vascular flora at four rarely used cemeteries within the Poznań city was confirmed in 2010. Apart from naturally occurring species, cultivated species were noted equally. Among species appearing spontaneously between the graves, species new for the flora of Poland: Chionodoxa forbesii, Ch. luciliae, Puschkinia scilloides, new for the flora of Wielkopolska: Bidens ferulifolius, Hyacinthoides hispanica and new for the flora of Poznań: Erigeron ramosus, Lilium bulbiferum, Pimpinella nigra, Poa subcaerulea, Veronica hederifolia s.s., were recorded. Names of taxa originating from cultivation are underlined.

  14. Plantas medicinales utilizadas en la salud infantil

    OpenAIRE

    Souza, ADZ.; T. Ceolin; Vargas, NRC.; Heck, RM.; Vasconcellos, CL.; Borges, AM.; Mendieta, MC.

    2011-01-01

    Identificar las plantas medicinales utilizadas en la salud infantil por familias de agricultores de base ecológica de la región Sur de Río Grande do Sul, Brasil. El enfoque de investigación es cualitativo, exploratorio y descriptivo, con datos recogidos de enero a mayo de 2009. Los sujetos fueron ocho familias de agricultores, residentes en los municipios de Pelotas, Morro Redondo, Canguçu y Arroio do Padre. Seis plantas fueron citadas (Chrysanthemun cinerariifolium, Foeniculum vulgare, Acca ...

  15. IN VITRO ANTIOXIDANT AND α-AMYLASE INHIBITION ACTIVITIES OF PANCHSAKAR CHURNA

    Directory of Open Access Journals (Sweden)

    Ashok Kumar B.S.

    2013-12-01

    Full Text Available Panchsakar Churna is the composition of Cassia angustifolia, Terminalia chebula, Zingiber officinale, Foeniculum vulgare and Saindhava lavana. Aqueous extract of churna was used to investigate antioxidant activity by ferrous ion chelating assay and ferric reducing power and alpha amylase inhibition activity by dinitrosalicylic acid method (DNSA. Aqueous extract of churna showed maximum ferrous chelating activity - 42.01 and ferric reducing power - 1.5 and 83.33 % of inhibition protein denaturation at 1000 µg/ml. Panchsakar churna showed significant antioxidant and alpha amylase inhibition activities.

  16. Aromatic plant-derived essential oil: an alternative larvicide for mosquito control.

    Science.gov (United States)

    Pitasawat, B; Champakaew, D; Choochote, W; Jitpakdi, A; Chaithong, U; Kanjanapothi, D; Rattanachanpichai, E; Tippawangkosol, P; Riyong, D; Tuetun, B; Chaiyasit, D

    2007-04-01

    Five aromatic plants, Carum carvi (caraway), Apium graveolens (celery), Foeniculum vulgare (fennel), Zanthoxylum limonella (mullilam) and Curcuma zedoaria (zedoary) were selected for investigating larvicidal potential against mosquito vectors. Two laboratory-reared mosquito species, Anopheles dirus, the major malaria vector in Thailand, and Aedes aegypti, the main vector of dengue and dengue hemorrhagic fever in urban areas, were used. All of the volatile oils exerted significant larvicidal activity against the two mosquito species after 24-h exposure. Essential oil from mullilam was the most effective against the larvae of A. aegypti, while A. dirus larvae showed the highest susceptibility to zedoary oil.

  17. Effects of botanical insecticides on the instantaneous population growth rate of Aphis gossypii Glover (Hemiptera: Aphididae) in cotton

    OpenAIRE

    Andrade, Lígia Helena de; Oliveira, José Vargas de; Breda, Mariana Oliveira; Marques,Edmilson Jacinto; Lima,Iracilda Maria de Moura

    2012-01-01

    Botanical insecticides have been studied aiming the alternative pest control. The present study investigated the effects of these insecticides on the instantaneous population growth rate (ri) of Aphis gossypii. Botanical insecticides were tested in the following concentrations: Compostonat®, Rotenat-CE® and Neempro (0, 0.50, 0.75, 1.00, 1.25, 1.50 and 1.75%); Natuneem® and Neemseto® (0, 0.25, 0.50, 0.75 and 1.00%) and essential oils of Foeniculum vulgare Mill., Cymbopogom winterianus (L.), Ch...

  18. Insecticidal and biochemical effect of some dried plants against Oryzaephilus surinamensis (Coleoptera-Silvanidae)

    OpenAIRE

    A.M. Al Qahtani; Z.M. Al-Dhafar; M.H. Rady

    2012-01-01

    Dry powders of three plants, namely ginger (Zingiber afficinale), hail (Elettaria cardamomum) and shammar (Foeniculum vulgare) were tested, for their toxicity, against the adult beetle Oryzaephilus surinamensis, as date pest threatens the date product in Saudi Arabia. All the tested plants showed insecticidal activity against O. surinamensis. Ginger is the most potent plant, recording the lowest LC50 value (0.14 mg/g) followed by hail and shammar (LC50 = 0.4 and 0.7 mg/g) respectively. Tested...

  19. 小茴香与伪品的鉴别研究

    Institute of Scientific and Technical Information of China (English)

    孙迎东; 汪风芹; 张静; 李风荣

    2005-01-01

    小茴香为伞形科植物茴香Foeniculum vulgare Mill.的干燥成熟果实。具散寒止痛、理气和胃之功,为临床常用中药。藏茴香、莳萝子、毒芹子、孜然和防风的种子作为小茴香的伪品在基层中药房时有出现。现将鉴别要点概述如下:

  20. 家庭百草园系列之十六小茴香

    Institute of Scientific and Technical Information of China (English)

    黄宝康; 梁婕

    2010-01-01

    @@ 小茴香(Foeniculum vulgate)为伞形科多年生草本植物,又名怀香、茴香.因能除肉之臭气,使之重新回香,故有"茴香"之名.小茴香原产欧洲地中海一带,现我国各地普遍有栽培.其植株高度0.6米~2米不等,全株表面有粉霜,植物有强烈的香气.

  1. 我国稀有蔬菜介绍(2)

    Institute of Scientific and Technical Information of China (English)

    朱立新; 徐践

    2002-01-01

    @@ (续2002年第4期62页) 5.球茎茴香 球茎茴香是伞形花科茴香属中茴香的一个变种[Foeniculum vulgareMill, var .azoricum(Mill)Thell],又称结球茴香,为二年生草木植物.原产地中海沿岸及西亚,我国近年引入,栽培较少,在北京、上海、广东、云南、湖北和浙江等地发展种植.

  2. Inhibition Mould of Distillation Extracts from Seven Species of Natural Plants%小茴香等7种植物蒸馏提取物的抑霉菌效果

    Institute of Scientific and Technical Information of China (English)

    王建清; 杨艳; 金政伟; 赵亚珠

    2011-01-01

    以存在于水果的常见霉菌中的灰霉、黑根霉、交链孢、青霉和黑曲霉为研究对象,采用滤纸片扩散法和时倍稀释法,对小茴香、柠檬草、孜然、广藿香、肉桂叶、桉树叶和鼠尾草这7种植物的蒸馏提取物有效抑菌成分(精油)进行抑菌筛选研究.结果表明:小茴香抑菌效果最好且得率高,为1.90%,对青霉、黑根霉最低抑菌浓度(MIC)均为3.2μL/mL,灰霉、黑曲霉和交链孢MIC为6.4μL/mL;其次为肉桂叶,对交链孢和灰霉有很强抑菌效果,9d内抑菌率为100%.%By filter paper diffusion method and dilution method,the inhibition characters of Foeniculum vulgare Mill,Cymbopogon citratus , Cuminum cyminum L., Pogostemon cablin Benth, Cinnamomum cassia Presl. , Eucalyptus leaves and Salvia japonica Thunb. essential oil extracted by steam distillation were studied. Common Botrytis cinerea,Rhizopus nigricans ,Alternaria.Penicillium citrinum,Aspergillus niger in the mould existing in fruits were chosed as research objects.The results show that Foeniculum vulgare Mill is the most effective bacteriostatic agent and has the highest extraction productivity which is 1.90%. Minimal inhibitory concentration of Foeniculum vulgare Mill effect on Penicillium citrinum and Rhizopus nigricans is 3.2 μL/mL. Minimal inhibitory concentration of Foeniculum vulgare Mill effect on Botrytis cinerea,Aspergillus niger,Alternaria is 6.4 μL/mL. The latter is Cinnamomum cassia Presl,especially to Botrytis cinerea and Alternaria has the best effect. The Inhibitory rate was 100% in 9 d.

  3. MYCOPOPULATION OF MEDICINAL PLANTS IN CROATIA

    Directory of Open Access Journals (Sweden)

    Karolina Vrandečić

    2011-12-01

    Full Text Available There has not been a systematic research of medicinal plants mycoflora in Croatia. This paper aims to present the results of preliminary research of mycopopulation of 14 species of medicinal plants. Total of 393 plant parts has been examined and 10 genera of fungi were isolated: Penicillium, Aspergillus, Sordaria, Phoma, Cladosporium, Rhizopus, Stemphillium, Fusarium, Phomopsis and one unidentified genus. Penicillium sp. (from 11 of 14 plant species was isolated from the majority of samples. The plants fungi were isolated from did not show any macroscopically visible symptoms of infection, except plant parts of Lavandula x intermedia and Foeniculum vulgare, from which Phomopsis sp. and Fusarium sp. were isolated

  4. 三种植物挥发油对菜青虫的拒食作用研究

    Institute of Scientific and Technical Information of China (English)

    吴利民; 原国辉; 陆宁海; 许永霞

    2005-01-01

    用水蒸气蒸馏法从莴苣(Lactucasativa L.)、茴香(Foeniculum vulgare MILL.)、荆芥(Schizonepeta tenuifolia Briq.)茎叶中提取其挥发油,采用叶碟浸渍法测定不同浓度对3龄菜青虫(Pieris rapae L.)的选择性与非选择性拒食作用.结果表明茴香、荆芥挥发油对菜青虫具有较强的拒食作用.

  5. Composición y capacidad antioxidante in-vitro de aceites esenciales ricos en Timol, Carvacrol, trans-Anetol o Estragol Composition and in-vitro antioxidant capacity of essential oils rich in Thymol, Carvacrol, trans-Anethole or Estragole

    Directory of Open Access Journals (Sweden)

    Amner Muñoz-Acevedo

    2009-12-01

    Full Text Available Se determinó por cromatografía de gases acoplada a espectrometría de masas (GC-MS la composición química de aceites esenciales (AE, aislados por hidrodestilación asistida por la radiación de microondas (MWHD, de las especies vegetales aromáticas Artemisia dracunculus, Foeniculum vulgare, Illicium verum, Lippia micromera, Lippia origanoides, Ocimum spp., Plectranthus amboinicus, Tagetes filifolia, Tagetes lucida y Thymus vulgaris. Los valores de capacidades antioxidantes in vitro de estos aceites esenciales, se obtuvieron usando los ensayos de decoloración del catión-radical ABTS+• (metodologías convencional y con dilución en microplacas y la oxidación del ácido linoleico, inducida por O2 y Fe+2. El potencial inhibitorio de ABTS+• fue más alto para los aceites esenciales que contienen fenoles (carvacrol y timol, que para los aceites esenciales ricos en éteres (trans-anetol y estragol. La actividad antioxidante mediante el ensayo ABTS+• modificado en orden decreciente fue: AE Plectranthus amboinicus ≥ AE Lippia origanoides >> AE Thymus vulgaris > AE Lippia micromera >>> AE Tagetes lucida (flores > AE Ocimum sp. > AE Tagetes lucida (hojas > AE Illicium verum > AE Tagetes filifolia (Cenivam > AE Foeniculum vulgare. Salud UIS 2009; 41: 287-294Gas chromatography coupled to mass spectrometry (GC-MS was used to determine the chemical composition of essential oils (EO isolated by microwave-radiation-assisted hydrodistillation (MWHD of Artemisia dracunculus, Foeniculum vulgare, Illicium verum, Lippia micromera, Lippia origanoides, Ocimum sp., Plectranthus amboinicus, Tagetes filifolia, Tagetes lucida and Thymus vulgaris. in vitro antioxidant capacity values using ABTS+• discoloration assays (traditional and microplate methods and the linoleic acid oxidation (with O2 and Fe+2 of these essential oils were obtained. Essential oils with phenols (carvacrol and thymol high content showed higher total antioxidant capacity values than

  6. Determination of trace elements in Syrian medicinal plants and their infusions by energy dispersive X-ray fluorescence and total reflection X-ray fluorescence spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Khuder, A. [Department of Chemistry, Atomic Energy Commission, P.O. Box 6091, Damascus (Syrian Arab Republic)], E-mail: scientific2@aec.org.sy; Sawan, M.Kh.; Karjou, J. [Department of Chemistry, Atomic Energy Commission, P.O. Box 6091, Damascus (Syrian Arab Republic); Razouk, A.K. [Department of Agriculture, Atomic Energy Commission, P.O. Box 6091, Damascus (Syrian Arab Republic)

    2009-07-15

    X-ray fluorescence (XRF) and total-reflection X-ray fluorescence (TXRF) techniques suited well for a multi-element determination of K, Ca, Mn, Fe, Cu, Zn, Rb, and Sr in some Syrian medicinal plant species. The accuracy and the precision of both techniques were verified by analyzing the Standard Reference Materials (SRM) peach-1547 and apple leaves-1515. A good agreement between the measured concentrations of the previously mentioned elements and the certified values were obtained with errors less than 10.7% for TXRF and 15.8% for XRF. The determination of Br was acceptable only by XRF with an error less than 24%. Furthermore, the XRF method showed a very good applicability for the determination of K, Ca, Mn, Fe, Cu, Zn, Rb, Sr, and Br in infusions of different Syrian medicinal plant species, namely anise (Anisum vulgare), licorice root (Glycyrrhiza glabra), and white wormwood (Artemisia herba-alba)

  7. Determination of trace elements in Syrian medicinal plants and their infusions by energy dispersive X-ray fluorescence and total reflection X-ray fluorescence spectrometry

    Science.gov (United States)

    Khuder, A.; Sawan, M. Kh.; Karjou, J.; Razouk, A. K.

    2009-07-01

    X-ray fluorescence (XRF) and total-reflection X-ray fluorescence (TXRF) techniques suited well for a multi-element determination of K, Ca, Mn, Fe, Cu, Zn, Rb, and Sr in some Syrian medicinal plant species. The accuracy and the precision of both techniques were verified by analyzing the Standard Reference Materials (SRM) peach-1547 and apple leaves-1515. A good agreement between the measured concentrations of the previously mentioned elements and the certified values were obtained with errors less than 10.7% for TXRF and 15.8% for XRF. The determination of Br was acceptable only by XRF with an error less than 24%. Furthermore, the XRF method showed a very good applicability for the determination of K, Ca, Mn, Fe, Cu, Zn, Rb, Sr, and Br in infusions of different Syrian medicinal plant species, namely anise ( Anisum vulgare), licorice root ( Glycyrrhiza glabra), and white wormwood ( Artemisia herba-alba).

  8. [Peculicidal activity of plant essential oils and their based preparations].

    Science.gov (United States)

    Lopatina, Iu V; Eremina, O Iu

    2014-01-01

    The peculicidal activity of eight plant essential oils in 75% isopropyl alcohol was in vitro investigated. Of them, the substances that were most active against lice were tea tree (Melaleuca), eucalyptus, neem, citronella (Cymbopogon nardus), and clove (Syzygium aromaticum) oils; KT50 was not more than 3 minutes on average; KT95 was 4 minutes. After evaporating the solvent, only five (tea tree, cassia, clove, anise (Anisum vulgare), and Japanese star anise (Illicium anisatum) oils) of the eight test botanical substances were active against lice. At the same time, KT50 and KT95 showed 1.5-5-fold increases. Citronella and anise oils had incomplete ovicidal activity. Since the lice were permethrin-resistant, the efficacy of preparations based on essential oils was much higher than permethrin.

  9. Effect of essential oils on Aspergillus spore germination, growth and mycotoxin production:a potential source of botanical food preservative

    Institute of Scientific and Technical Information of China (English)

    Negero Gemeda; Yimtubezinash Woldeamanuel; Daniel Asrat; Asfaw Debella

    2014-01-01

    Objective: To investigate effect of essential oils on Aspergillus spore germination, growth and mycotoxin production.Method: In vitro antifungal and antiaflatoxigenic activity of essential oils was carried out using poisoned food techniques, spore germination assay, agar dilution assay, and aflatoxin arresting assay on toxigenic strains of Aspergillus species.Results: Cymbopogon martinii, Foeniculum vulgare and Trachyspermum ammi (T. ammi) essential oils were tested against toxicogenic isolates of Aspergillus species. T. ammi oil showed highest antifungal activity. Absolute mycelial inhibition was recorded at 1 µl/mL by essential oils of T. ammi. The oil also showed, complete inhibition of spore germination at a concentration of 2 µl/mL. In addition, T. ammi oil showed significant antiaflatoxigenic potency by totally inhibiting aflatoxin production from Aspergillus niger and Aspergillus flavus at 0.5 and 0.75 µl/mL, respectively. Cymbopogon martinii, Foeniculum vulgare and T. ammi oils as antifungal were found superior over synthetic preservative. Moreover, a concentration of 5 336.297 µl/kg body weight was recorded for LC50 on mice indicating the low mammalian toxicity and strengthening its traditional reputations.Conclusions:In conclusion, the essential oils from T. ammi can be a potential source of safe natural food preservative for food commodities contamination by storage fungi.

  10. Total Phenolic, Flavonoids and Antioxidant Capacity of Some Medicinal and Aromatic Plants

    Directory of Open Access Journals (Sweden)

    Melinda Nagy

    2014-11-01

    Full Text Available Abstract: Antioxidants are substances that protect cells from the induced oxidative stress damage caused by unstable molecules known as free radicals that. Antioxidants neutralize free radicals as a natural by-product of normal cell processes. In the present study,were evaluated  the phenolic and flavonoids contents as well as the antioxidant capacity of seeds from  the Lamiaceae and Apiaceae family: fennel  (Foeniculum vulgare, dill (Anethum graveolens and rosemary (Rosmarinus officinalis . (Sreemoyee Ch. et. al., 2012 The main objective of the study was the comparative assessment of the phenolic and flavonoid compounds from dill, rosemary and fennel methanolic extracts correlated with their  antioxidant activity. Both total phenolic content and flavonoids content of the seeds samples were measured spectrophotometrically using the Folin-Ciocalteu assay and a chromogenic system of NaNO2–Al(NO33–NaOH, respectively.. Antioxidant capacity was determined by 2,2-DPPH method. Results strongly showed that Rosmarinus officinalis extract has the most effective antioxidant capacity in scavenging DPPH radicals, while Foeniculum vulgare and Anethum graveolens were less active. The total phenolic content was within 773,14 and 3367,24mg GAE/ 100g while the concentration in flavonoids was between 231,84 and 1325,53 QEg/100g dry seeds.  

  11. A database on endemic plants at Tirumala hills in India

    Science.gov (United States)

    Latheef, Shaik Abdul; Prasad, Beerkam; Bavaji, Middi; Subramanyam, Gangapatnam

    2008-01-01

    Medicinal plants play an important role in health care. The use of medicinal plants for treatment is growing in view of cost and non-compliance of modern medicine as in case of non-communicable diseases. Plants such as Boswellia, ovalifoliolata, Cycas beddomei, Pimpinella tirupatiensis, Pterocarpus santalinus, Shorea thumbuggaia, Syzygium alternifolium, Terminalia pallida are endemic to Tirumala hills of seshachalam range falling under the Eastern Ghats of India. These plants species have medicinal properties such as anti-tumorogenic, anti-microbial, purgative, hypoglycemic, abortificient, analgesic, anti-septic, anti-pyretic and anti-inflammatory. We created a database named DEPTH in an attempt to communicate data of these plants to the scientific community. DEPTH contains data on scientific name, vernacular name, family name, morphological description, economic importance, known medicinal compounds and medicinal importance. Availability http://svimstpt.ap.nic.in/MedicinalPlants/mainpage.htm PMID:18317578

  12. Fortification of yogurts with different antioxidant preservatives: A comparative study between natural and synthetic additives.

    Science.gov (United States)

    Caleja, Cristina; Barros, Lillian; Antonio, Amilcar L; Carocho, Márcio; Oliveira, M Beatriz P P; Ferreira, Isabel C F R

    2016-11-01

    Consumers demand more and more so-called "natural" products and, therefore, the aim of this work was to compare the effects of natural versus synthetic antioxidant preservatives in yogurts. Matricaria recutita L. (chamomile) and Foeniculum vulgare Mill. (fennel) decoctions were tested as natural additives, while potassium sorbate (E202) was used as a synthetic additive. The fortification of yogurts with natural and synthetic antioxidants did not cause significant changes in the yoghurt pH and nutritional value, in comparison with control samples (yogurt without any additive). However, the fortified yogurts showed higher antioxidant activity, mainly the yogurts with natural additives (and among these, the ones with chamomile decoction). Overall, it can be concluded that plant decoctions can be used to develop novel yogurts, by replacing synthetic preservatives and improving the antioxidant properties of the final product, without changing the nutritional profile. Copyright © 2016 Elsevier Ltd. All rights reserved.

  13. Wild food plants used on the Dubrovnik coast (south-eastern Croatia

    Directory of Open Access Journals (Sweden)

    Katija Dolina

    2014-09-01

    Full Text Available Croatia’s versatile plant use traditions are still not sufficiently documented. The aim of this study was to record local traditions of wild food plant use on the Dubrovnik coast. We interviewed 40 inhabitants of 23 villages, mainly knowledgeable informants. On average 19 species were listed, which in total produced an inventory of 95 food plant species (including species whose leaves or inflorescences are used as recreational teas. The most commonly collected are: Sonchus oleraceus and S. asper, Asparagus acutifolius, Dioscorea communis, Cichorium intybus, Crepis zacintha, Allium ampeloprasum, Picris echioides and Foeniculum vulgare (all of them used as vegetables, the fruits of Rubus ulmifolius (mainly eaten raw, the fruits of roses (Rosa sempervirens and R. canina and the leaves of Salvia officinalis (both roses and salvia are used for making recreational teas. A particular feature of the local gastronomy is the collection of young Ruscus aculeatus shoots.

  14. The effect of essential oils of sweet fennel and pignut on mortality and learning in africanized honeybees (Apis mellifera L.) (Hymenoptera: Apidae)

    Energy Technology Data Exchange (ETDEWEB)

    Abramson, Charles I.; Michaluk, Lynnette M. [Oklahoma State University, Stillwater, OK (United States). Depts. of Psychology and Zoology. Lab. Comparative Psychology and Behavioral Biology]. E-mail: charles.abramson@okstate.edu; Wanderley, Paulo A.; Wanderley, Maria J.A.; Silva, Jose C.R. [Universidade Federal da Paraiba (UFPB), Bananeiras, PB (Brazil). Dept. de Agricultura

    2007-11-15

    It was recently discovered that exposure to small concentrations of the essential oils of sweet fennel (Foeniculum vulgare Mill) or pignut [Hyptis suaveolens (L.) Poit] can be used to control aphids. What is not known is whether these oils also influence honeybee behavior. Experiments using both harnessed and free-flying foragers at concentrations used to control aphids showed that bees readily associated the odors with a reward, discriminated between them, and were not repelled. Honeybees, however, would not consume the oils when mixed with sucrose to create an unconditioned stimulus. An experiment in which harnessed bees consumed various concentrations showed that concentrations greater than 50% were detrimental. The experiments reported here provide further evidence supporting the use of conditioning techniques to evaluate the use of essential oils on honey bee behavior. (author)

  15. Wild food plants used in the villages of the Lake Vrana Nature Park (northern Dalmatia, Croatia

    Directory of Open Access Journals (Sweden)

    Łukasz Łuczaj

    2013-12-01

    Full Text Available Croatia is a country of diverse plant use traditions, which are still insufficiently documented. The aim of this study was to document local traditions of using wild food plants around Lake Vrana (northern Dalmatia, Zadar region.  We interviewed 43 inhabitants of six traditional villages north of Lake Vrana. On average 12 species were listed, which in total produced an inventory of 55 food plants and 3 fungi taxa. Wild vegetables were most widely collected, particularly by older women who gathered the plants mainly when herding their flocks of sheep. Wild fruits and mushrooms were rarely collected. The former used to be an important supplementary food for children, or for everyone during times of food shortage, and the latter were relatively rare due to the dry climate and shortage of woods. The most commonly collected plants are wild vegetables: Cichorium intybus, Foeniculum vulgare, Sonchus oleraceus, Asparagus acutifolius, Papaver rhoeas, Rumex pulcher, Daucus carota, Allium ampeloprasum and Silene latifolia.

  16. Comparison of various extraction techniques for isolation and determination of isoflavonoids in plants.

    Science.gov (United States)

    Bajer, Tomás; Adam, Martin; Galla, Lubomír; Ventura, Karel

    2007-01-01

    In the present paper, the following extraction techniques have been used for extracting isoflavonoids from the species Matricaria recutita, Rosmarinus officinalis, Foeniculum vulgare, and Agrimonia eupatoria L.: supercritical fluid extraction (SFE), pressurized fluid extraction, matrix solid phase dispersion, ultrasonic extraction in an ultrasonic bath (USE) and by means of an ultrasonic homogeniser (HOM), extraction by means of Soxhlet apparatus (SOX), and solid phase extraction. Experimental optimization of all techniques has been carried out using a soybean flour. Subsequent analyses of the extracts were carried out by liquid chromatography with UV detection. The maximum yields of daidzein and genistein were obtained by extraction with the SOX, USE, and HOM techniques. The maximum yields of apigenin and biochanin A from herb samples were obtained by SFE.

  17. Antioxidant and Antiacetylcholinesterase Activities of Some Commercial Essential Oils and Their Major Compounds

    Directory of Open Access Journals (Sweden)

    Smail Aazza

    2011-09-01

    Full Text Available The commercial essential oils of Citrus aurantium L., Cupressus sempervirens L., Eucalyptus globulus Labill., Foeniculum vulgare Mill. and Thymus vulgaris L., isolated by steam distillation by a company of Morocco were evaluated in terms of in vitro antioxidant activity through several methods. In vitro acetylcholinesterase inhibitory activity was also determined. Citrus limon (L. Burm. f. oil was also studied, but it was obtained by peel expression. The best antioxidant was T. vulgaris oil, independent of the method used, mainly due to the presence of the phenolic monoterpenes thymol and carvacrol, which when studied as single compounds also presented the best activities. Concerning the acetylcholinesterase inhibition activity, E. globulus was the most effective. Nevertheless its main components 1,8-cineole and limonene were not the most active, a feature that corresponded to d-3-carene.

  18. Repellency and toxicity of aromatic plant extracts against the mosquito Culex pipiens molestus (Diptera: Culicidae).

    Science.gov (United States)

    Traboulsi, Abdallah F; El-Haj, Samih; Tueni, Marie; Taoubi, Khalil; Nader, Natalie Abi; Mrad, Abir

    2005-06-01

    The insecticidal activities of essential oil extracts from leaves, flowers and roots of aromatic plants against fourth-instar larvae of the mosquito Culex pipiens molestus Forskal were determined. Extracts of Foeniculum vulgare Mill were the most toxic, followed by those of Ferula hermonis Boiss, Citrus sinensis Osbeck, Pinus pinea L, Laurus nobilis L and Eucalyptus spp with LC50 values of 24.5, 44.0, 60.0, 75.0, 117.0 and 120.0 mg litre(-1), respectively. Combination tests between the LC50 and the maximum sub-lethal concentration (MSLC) were determined. Over 20 major components were identified in extracts from each plant species tested. Five essential oils and nine pure components were studied for their repellency against mosquito bites. Terpineol and 1,8-cineole were the most effective against Culex pipiens molestus bites offering complete protection for 1.6 and 2 h, respectively.

  19. Insecticidal and biochemical effect of some dried plants against Oryzaephilus surinamensis (Coleoptera-Silvanidae

    Directory of Open Access Journals (Sweden)

    A.M. Al Qahtani

    2012-01-01

    Full Text Available Dry powders of three plants, namely ginger (Zingiber afficinale, hail (Elettaria cardamomum and shammar (Foeniculum vulgare were tested, for their toxicity, against the adult beetle Oryzaephilus surinamensis, as date pest threatens the date product in Saudi Arabia. All the tested plants showed insecticidal activity against O. surinamensis. Ginger is the most potent plant, recording the lowest LC50 value (0.14 mg/g followed by hail and shammar (LC50 = 0.4 and 0.7 mg/g respectively. Tested plants alter the protein configuration of O. surinamensis after using PAGE for protein analysis. Ginger and shammar increased the insect protein subfractions than normal; while hail reduced separated bands, especially proteins of moderate molecular weight.

  20. Gamma radiation (Co{sup 60}) effects on active substances and microbe burden of medicinal plants; Avaliacao dos efeitos da radiacao gama (Co{sup 60}) sobre principios ativos e carga microbiana de plantas medicinais

    Energy Technology Data Exchange (ETDEWEB)

    Dall' Agnol, L. [Herbarium Laboratorio Botanico Ltda, Colombo, PR (Brazil). Dept. de Garantia da Qualidade

    2001-04-01

    In order to evaluate the effects of radioactivity on active vegetal substances, samples of Fennel (Foeniculum vulgare Miller., fructus), Guarana (Paulinia cupana, Kunth, semen), Gingko (gingko biloba, L., folium), and Kawa-Kawa (Piper methysticum G. Forst, rhizoma), were treated with scaling doses (0 to 25 KGy) of gamma radiation (Co{sup 60}). The 'blind test' methodology was used. The active substances from each sample were analysed by qualitative and quantitative methods after radiation. There were no significant differences seen between the control sample (0 KGy) and the irradiated samples. Microbe contamination was significantly reduced, about 10000 CFU/g, with the initial 5 KGy dose. It was concluded that gamma radiation can be used as an alternative procedure to reduce microbiologic contamination in medicinal plants. Before this procedure can be extended to other medicinal plants, more specific analytical methods are recommended to verify possible structural alterations in active vegetal molecules. (author)

  1. 球茎茴香功效及其栽培

    Institute of Scientific and Technical Information of China (English)

    韩学俭

    2003-01-01

    @@ 一、功效与分布 球茎茴香(Foeniculum vulgare var.dulce Batt.et Trab.)英文名Horencefenndl.bulb fennel,别名结球茴香、意大利茴香、甜茴香,为伞形科(Umbell oferae)茴香属茴香的一个变种,属一二年生草本植物.原产意大利南部,主要分布于地中海沿岸和西亚.我国从20世纪70年代就从意大利引入.目前,北京、天津、广东、四川等省市有栽培.

  2. 小茴香挥发油提取方法比较

    Institute of Scientific and Technical Information of China (English)

    杨红

    2004-01-01

    @@ 小茴香为伞形科植物茴香Foeniculum vulgare Mill的干燥成熟果实,是常用中药之一.性温,味辛,有祛寒止痛、理气和胃之功效,含挥发油3%~8%,油中主含茴香醚约50%~60%,D-茴香酮约18%~20%,甲基胡椒酚约10%,尚含少量柠檬烯、D-α-松油烯、茴香醛、β-香叶烯、α-水芹烯等[1].传统小茴香挥发油的提取方法是水蒸气蒸馏法,但它有许多缺点,如有效成分易破坏,提取时间长等.

  3. Cottage cheeses functionalized with fennel and chamomile extracts: Comparative performance between free and microencapsulated forms.

    Science.gov (United States)

    Caleja, Cristina; Ribeiro, Andreia; Barros, Lillian; Barreira, João C M; Antonio, Amilcar L; Beatriz P P Oliveira, M; Barreiro, Maria Filomena; Ferreira, Isabel C F R

    2016-05-15

    Globally, there is a trend for healthy food products, preferably incorporating natural bioactive ingredients, replacing synthetic additives. From previous screening studies, extracts of Foeniculum vulgare Mill. (fennel) and Matricaria recutita L. (chamomile) maintained nutritional properties and improved the antioxidant activity of cottage cheese. Nevertheless, this effect was limited to 7 days. Accordingly, aqueous extracts of these plants were microencapsulated in alginate and incorporated into cottage cheese to achieve an extended bioactivity. Plain cottage cheese, and cheese functionalized by direct addition of free decoctions, were prepared and compared. Independently of plant species, "functionalization type" factor did not show a significant effect on the nutritional parameters, as also confirmed in the linear discriminant analysis, where these parameters were not selected as discriminating variables. Furthermore, samples functionalized with microencapsulated extracts showed higher antioxidant activity after the 7th day, thereby demonstrating that the main purpose of this experimental work was achieved.

  4. 小茴香及其混淆品莳萝子的鉴别

    Institute of Scientific and Technical Information of China (English)

    邓君丽

    1998-01-01

    @@ 小茴香为常用中药,系伞形科植物茴香Foeniculum vulgare Mill.的干燥成熟果实.有些地区则把同科植物莳萝Anethum graveolens L.的干燥果实作小茴香入药.据文献[1~3]记载,小茴香和莳萝子是功效不同的2种中药,不宜混淆使用.为保证临床用药的准确,本文从性状、紫外吸收光谱和薄层色谱三方面进行了比较鉴别.

  5. 小茴香与藏茴香的鉴别%Identification of Fennel and Caraway

    Institute of Scientific and Technical Information of China (English)

    李纳英; 毕焕新

    2008-01-01

    小茴香为伞形科植物茴香Foeniculum vulgate Mill.的干燥成熟果实,为常用中药。始载于《唐本草》,又名茴香、谷茴香。气香,性味辛、温。小茴香果实含挥发油,主要成份含茴香醚、α-茴香酮、茴香醛等。具有散寒止痛,理气和胃之功能.藏茴香为伞形科植物黄蒿Carum carvi L.的种子,又名黄蒿、马缨子、小防风。民族药名有棵虐、亚瓦比迪扬等。性味辛、涩、温。

  6. 香精香料信息17则

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    1.不同氮肥剂量对小茴香农学和化学性质的影响 土耳其YuzuncuYil大学农学院学者M.Tuncturk在“Asian Journal of Chemistry”杂志2008,20(4),3209~3217页上用英文发表的文章报道作者以四种不同的氮肥(0、20、40和60kg/公顷)来研究对小茴香(Foeniculum vulgare Mill.)得率和质量的影响。

  7. 小茴香化学成分及药理活性研究进展%Research Progress of ChemicaI Constituents and PharmacoIogicaI Activity of Foeniculumvulgare Mill

    Institute of Scientific and Technical Information of China (English)

    董思敏; 张晶

    2015-01-01

    Foeniculumvulgare Mill. is a plant that has the concomitant function of medicine and food, which is not only a kind of general-adopted seasoning,but also traditional Chinese medicine.Through consulting a large number of related literature, mainly summarize the chemical constituents, pharmacological activity and development and utilization of Foeniculum vulgare Mill.,aiming at providing reference for further study and rational use of Foeniculumvulgare Mill..%小茴香是一种食药同源的植物,既是常用的调味品,也是传统的中药。通过查阅大量相关文献,对小茴香的化学成分、药理活性、开发利用研究现状进行综述,旨为小茴香的深入研究及其合理利用提供参考。

  8. Electrophoretic enzyme analysis of North American and eastern Asian populations of Agastache sect. Agastache (Labiatae)

    Science.gov (United States)

    Vogelmann, James E.; Gastony, Gerald J.

    1987-01-01

    Genetic relationships among the seven species of Agastache sect. Agastache common in North America and the one found in eastern Asia were assessed using starch-gel electrophoresis of twelve enzymatic proteins. Nei's (1976) genetic distance and identity values, calculated among the 32 populations used in this study, partitioned the Agastache section into four discrete groups: (1) A. nepetoides (eastern North America), (2) A. scrophulariifolia and A. foeniculum (eastern and central North America), (3) the four species of the western U.S. (A. urticifolia, A. occidentalis, A. parvifolia, and A. cusickii), and (4) A. rugosa (eastern Asia). The Asian Agastache, separated from its American congeners for over 12 million years, differed from American populations at only two (the IDH-1 and LAP-1 alleles) of the fifteen loci surveyed; these alleles were not found in any of the North American plants. Nei's genetic distances between the Asian and North American populations ranged from 0.2877 to 0.6734.

  9. Activities of Apiaceae essential oils against armyworm, Pseudaletia unipuncta (Lepidoptera: Noctuidae).

    Science.gov (United States)

    Sousa, Rose Marie O F; Rosa, José S; Oliveira, Luisa; Cunha, Ana; Fernandes-Ferreira, Manuel

    2013-08-14

    Essential oils (EOs) from four Apiaceae species and 11 pure compounds were evaluated for their antifeedant, growth inhibitory, and insecticidal activities against Pseudaletia unipuncta (Lepidoptera: Noctuidae) fourth-instar larvae. EOs from Foeniculum vulgare subsp. vulgare var. vulgare, Anethum graveolens , Petroselinum crispum , and Cuminum cyminum were characterized by gas-chromatography (GC) and mass spectrometry. Anti-insect activity varied according to plant specie/composition, type, and exposure period. EOs from P. crispum and A. graveolens fruits, trans-anethole and cuminaldehyde, exerted acute effects on larvae feeding and growth (FDI and GI > 70%). A. graveolens , C. cyminum , and F. vulgare EOs and some of their constituents were effective by fumigation (≥ 80%). Satisfactory contact toxicities (>70%) were observed for five compounds and all EOs, except F. vulgare EOs, when tested by the filter paper impregnation method. For the most active EOs/compounds, dose-dependent toxicity was determined and inverse relationships of LC50 with time were established.

  10. [Influence of spiced feed additives on taste of hen's eggs].

    Science.gov (United States)

    Richter, Th; Braun, P; Fehlhaber, K

    2002-01-01

    The effect of the spices garlic (Allium sativum), extract from garlic, sage (Salvia officinalis), caraway (Carum carvi), peppermint (Mentha piperita), fennel (Foeniculum vulgare), thyme (Thymus vulgaris), paprika (Capsicum annuum), marjoram (Majorana hortensis), cardamom (Elettaria cardamomum) and sausage-mix fed to white hybrides on the quality of eggs (n = 705) was tested by 10 qualified persons. Changes in smell or taste were partly significant; a direct improvement is hard to induce. A well seasoned taste was obtained by feeding of garlic, fennel, peppermint and marjoram. They produce an aromatic flavor. A practical application is possible but limited by higher production prices. Further, it is uncertain if there is a need for "taste-manipulated" eggs.

  11. Inhibitory effects of various essential oils and individual components against extended-spectrum beta-lactamase (ESBL) produced by Klebsiella pneumoniae and their chemical compositions.

    Science.gov (United States)

    Orhan, Ilkay Erdogan; Ozcelik, Berrin; Kan, Yüksel; Kartal, Murat

    2011-10-01

    In the current study, in vitro inhibitory activity of several essential oils obtained from the cultivated plants, Foeniculum vulgare, Mentha piperita and M. spicata, Ocimum basilicum, Origanum majorana, O. onites, O. vulgare, Satureja cuneifolia, and a number of individual essential oil components of terpene and aromatic types were screened against 10 isolated strains of Klebsiella pneumoniae producing extended-spectrum beta-lactamase (ESBL) enzyme, which makes this microorganism quite resistant against the antibiotics: trimetoprime-sulfametoksazol, sulbactam-ampicilin, clavulonate-amoxicilin, ceftriaxon, cefepime, imipenem, ceftazidime, tobramicine, gentamisine, ofloxacin, and ciprofloksasin. All of the essential oils and the components exerted a remarkable inhibition ranging between 32 and 64 μg/mL against all of these strains as strong as the references (ampicilin and oflaxocin) inhibiting at 32 μg/mL. Besides, chemical compositions of the essential oils were elucidated by gas chromatography-mass spectrometry (GC-MS). The essential oils and the pure components widely found in essential oils screened herein have shown remarkable inhibition against ESBL-producing K. pneumoniae strains, which leads to the suggestion that they may be used as food preservatives for this purpose. Practical Application:  The essential oils obtained from Foeniculum vulgare, Mentha piperita and M. spicata, O.cimum basilicum, Origanum majorana, O. onites, O. vulgare, and Satureja cuneifolia as well as common essential oil components have shown notable inhibitory effects against 10 isolated strains of Klebsiella pneumoniae producing extended-spectrum beta-lactamase (ESBL) enzyme and they might be used as food preservative or ingredient. © 2011 Institute of Food Technologists®

  12. Evaluation of antibacterial activity of selected Iranian essential oils against Staphylococcus aureus and Escherichia coli in nutrient broth medium.

    Science.gov (United States)

    Mohsenzadeh, Mohammad

    2007-10-15

    The antibacterial effect of different concentrations (0.01 to 15%) of thyme (Thymus vulgaris), peppermint (Mentha piperita L.) caraway seed (Carum carvi), fennel (Foeniculum vulgar), tarragon (Artmesia dracunculus) and pennyroyal (Mentha pullegium) essential oils on the Staphylococcus aureus and Escherichia coli was studied in nutrient broth medium. The MIC values of peppermint, fennel, thyme, pennyroyal and caraway essential oils against Escherichia coli were 0.5 +/- 0.03, 1 +/- 0.03, 0.3 +/- 0.01, 0.7 +/- 0.03 and 0.6 +/- 0.02% and in contrast, for Staphylococcus aureus were 0.4 +/- 0.01, 2 +/- 0.13, 0.1 +/- 0.01, 0.5 +/- 0.02 and 0.5 +/- 0.02%, respectively. The MBC values of peppermint, fennel, thyme, pennyroyal and caraway essential oils for Escherichia coli were 0.7 +/- 0.02, 2 +/- 0.05, 0.5 +/- 0.02, 1 +/- 0.02 and 0.8 +/- 0.02 and for Staphylococcus aureus were 0.5 +/- 0.02, 4 +/- 0.26, 0.3 +/- 0.02, 0.7 +/- 0.02 and 0.6 +/- 0.01, respectively. Statistical evaluation of the results indicated that the essential oils of thyme (Thymus vulgaris) showed the broadest spectrum of action (p Essential oils of peppermint (Mentha piperita), caraway seed (Carum carvi), pennyroyal (Menthae pullegium) and fennel (Foeniculum vulgar) had moderate effect against tested microorganisms and in contrast, tarragon essential oil were less effective against tested microorganisms. In conclusion, essential oils of edible plants could be a potential source for inhibitory substances for some foodborne pathogens. Natural substances that extracted from plants have applications in controlling pathogens in foods.

  13. Comparison of melissa with citalopram and placebo in treatment of sleep disorders in menopausal women: clinical trial

    Directory of Open Access Journals (Sweden)

    Mahboobeh Shirazi

    2016-11-01

    Full Text Available Background: Different treatment used for resolving menopausal problems. Some studies assayed effectiveness of citalopram but it had some side effects and other studies about medicinal plants in Iran, including Melissa (combination of officinalis and foeniculum vulgare showed improvement insomnia and anxiety. This study decided to assay effectiveness of this drug and comparison with placebo and citalopram in treatment of sleep disturbance of menopausal women. Methods: Sixty postmenopausal women suffering from sleep disturbances that referred to Yas hospital between 2011-2013 were recruited to this double blind controlled study with 8 weeks’ follow-up period. They were randomized in three groups of twenty patients each, group A: received Melissa 600 mg that made by traditional medical school, group B: received citalopram 20 mg from Arya company that increased to 30 mg after one week and group C: received placebo. The patients were evaluated by Pittsburgh Sleep Quality Index (PSQI questionnaire before and after treatment, also we checked the side effects of every drugs. Study was dissertation of one of the author with code 22263. This research has been supported by Tehran University of Medical Sciences and Health Services Grant. This study was registered at Iranian Registry of Clinical Trials with code of IRCT2013072714174N1. Results: Pittsburgh sleep quality index improved significantly in all groups, there was significant differences between Melissa group and two other group, but there wasn’t significance difference between citalopram and placebo group, there was a trend in favor of Melissa versus citalopram and placebo. All of seven field of PSQI improved significantly in all groups that showed improvement of sleep quality in all field of sleep disturbance. Conclusion: Melissa (compound of officinalis and foeniculum vulgare may be recommended for the treatment of sleep disturbances in postmenopausal women. Although further investigation with

  14. Empleo de aceites esenciales como antioxidantes naturales

    Directory of Open Access Journals (Sweden)

    Zydgalo, J. A.

    1995-10-01

    Full Text Available The aims of this study were to determine and compare the antioxidant effect of essential oils from Thymus vulgaris, Laurus nobilis, Foeniculum vulgare, Eucalyptus globules, Tagetes minuta, Satureja parvifolia and Lippia polystachya in soybean oil. The concentrations of volatile oils in soybean oil were 0.1% and 0.02%. The antioxidant activity was measured by peroxide values. Thymus vulgaris and L. nobilis essential oils exhibited a significant antioxidant activity, followed by F. vulgare and E. globules in a decreasing order. Tagetes minuta, S. parvifolia and L. polystachya had not effect, and their contribution to the stability of soybean oil was negligible.

    Los objetivos de este estudio fueron determinar y comparar el efecto antioxidante de los aceites esenciales de Thymus vulgaris, Laurus nobilis, Foeniculum vulgare, Eucalyptus globulus, Tagetes minuta, Satureja parvifolia y Lippia polystachya en el aceite de soja. Las concentraciones de las esencias en el aceite de soja fueron 0.1% y 0.02%. La actividad antioxidante fue medida por los valores de peróxido. Los aceites esenciales de T. vulgaris y L nobilis exhibieron una significativa acción antioxidante seguidos, en orden decreciente, por F. vulgare y E. globulus. Las esencias de T. minuta, S. parvifolia y L polystachya no mostraron efecto antioxidante y su contribución a la estabilidad oxidativa del aceite de soja fue despreciable.

  15. AROMATIC PLANT OF LAMIACEAE FAMILY FOR USE IN PHYTOTHERAPY

    Directory of Open Access Journals (Sweden)

    L. A. Logvinenko

    2016-01-01

    Full Text Available Introduction. Therapeutic action of terpenoids of essential oil in uncommon plants like Elsholtzia stauntonii, Agastache foenic-ulum, Monarda fistulosa, Perovskia atri-plicifolia, Majorana hortensis, therefore the study for these plants application in phyto-therapy is timely. The study for componen-tial content of essential oil of 18 uncommon species from Lamiaceae family from the collection of Nikitsky Botanical Garden to increase therapeutic possibilities and their practical use in phytotherapy was the purpose of this paper. The plant species under study were introduced to the South Coast of Crimea from different ecological and geographic zones in seeds from botanical gardens of Europe. Methods. Every species was represented by 3-20 samples, which were studied by biomorpholofical and principal economically valuable features. The article presents the generalization and analysis of long-time studies for biologically active substances from 18 species of aromatic plants from Lamiaceae family. The content of essential oil was determined in a phase of mass blossom, from freshly harvested raw materials by means of hydro distillation at Ginsberg apparatuses. Componential content of essential oil was studied by the method of gas liquid chromatography. Results. Identification of the essential oils components allowed isolation of plant chemotypes with high content of valuable substances (methyl chavicol, carvacrol, pinen, thymol, and others. Highproductive samples (forms, prospective for industrial cultivation were isolated by the features complex. Based on the classification of A.D. Turova in phytotherapeutic action of plants and results of our studies, the isolated plant forms were distributed in 10 groups of usage in phytotherapy. On the example of Agastache foeniculum we showed that one and the same plant species may have different chemotypes, which have treatment and toxic action. Conclusion. The data obtained broaden and add the information about a

  16. Influence of several plant extracts on the oviposition behaviour of Aedes fluviatilis (Lutz (Diptera: Culicidae in the laboratory Influência de diversos extratos vegetais sobre o comportamento de oviposição de Aedes fluviatilis (Lutz (Diptera: Culicidae em laboratório

    Directory of Open Access Journals (Sweden)

    Rotraut A. G. B. Consoli

    1989-03-01

    Full Text Available Whole, ethanolic, hexanic, lyophilized extracts of several plants and anacardic acid tested in respect of their influence on the oviposition behavior of Aedes fluviatilis (Lutz at 100, 10 and 1 ppm concentrations. Extracts of Allium stivum, Jatropha curcas, Mikania schenkii, Poinciana regia and Spatodea campanulata had a repulsive effect (α=0.05 on females at 100 ppm, those of Anacardium occidentale, Bidens segetum and Caesalpinia peltophoroides were also repelent at 10 ppm. Extracts of Coriandrum sativum (100, 10 and 1 ppm, Chara Zeylanica (10 ppm, Cupressus sempervirens (10 ppm, Foeniculum vulgare (10 ppm and Spatodea campanulata (1 ppm were attractive to the females; 13 (52.0% of the extracts tested, did not influence the oviposition behavior.Extratos brutos, etanólicos, hexânicos, liofilizados de diversos vegetais e ácido anacárdico foram testados quanto a sua influência sobre o comportamento de oviposição das fêmeas de Aedes fluviatilis (Lutz, nas concentrações de 100, 10 e 1 ppm. Os estratos de Allium sativum, jatropha curcas. MiKania schenkii, Poinciana regia s Spatodea campanulata mostraram-se repelentes (α = 0,05 para as fêmeas na concentração de 100 ppm e os de Anacardium occidentale, Bidens segetum e Caesalpinia peltophoroides também na de 10 ppm. Os extratos Coriandrum stivum (100, 10 e 1 ppm, chara zeylanica (10 ppm, Cupressus sempervirens (10 ppm, Foeniculum vulgare (10 ppm e Spatodea campanulata (1 ppm atrairam a oviposição das fêmeas; 13 (52,0% dos extratos testados foram indiferentes as fêmeas nas concentrações utilizadas.

  17. MORPHOLOGICAL AND MOLECULAR IDENTIFICATION OF Fusarium SPECIES AND THEIR PATHOGENICITY FOR WHEAT

    Directory of Open Access Journals (Sweden)

    Jelena Poštić

    2012-12-01

    Full Text Available From the root and lower stem parts of weeds and plant debris of maize, wheat, oat and sunflower we isolated 300 isolates of Fusarium spp. and performed morphological and molecular identification. With molecular identification using AFLP method we determined 14 Fusarium species: F. acuminatum, F. avenaceum, F. concolor, F. crookwellense, F. equiseti, F. graminearum, F. oxysporum, F. proliferatum, F. semitectum, F. solani, F. sporotrichioides, F. subglutinans, F. venenatum and F. verticillioides.By comparing results of morphological and molecular identification we found out that determination of 16,7% isolates was incorrect. Out of 300 isolates identified with molecular methods, 50 did not belong to the species determined with morphological determination.With pathogenicity tests of 30 chosen Fusarium isolates we determined that many of them were pathogenic to wheat and maize seedlings and to wheat heads. The most pathogenic were isolates of F. graminearum from A. retroflexus, A. theophrasti and C. album, F. venenatum from maize debris and and A. theophrasti, F. crookwellense from A. lappa. Antifungal influence of 11 essential oils on mycelia growth and sporulation of chosen Fusarium isolates determined that essential oils of T. vulgaris, P. anisum and E. caryophyllus had the strongest effect on mycelial growth. Influence of essential oils on sporulation was not statistically significant.

  18. Possibilities for production of medicinal aromatic plants on the spoil bank of the Pljevlja coal mine. Mogucnost gajenja ljekovitog i aromaticnog bilja na jalovistu rudnika uglja - Pljevlja

    Energy Technology Data Exchange (ETDEWEB)

    Memic, M.; Bajovic, L. (Rudnik Uglja Pljevlja (Yugoslavia))

    1990-01-01

    Presents results obtained from experimental recultivation of the Potrlica surface coal mine spoil bank (810 m elevation, continental climate) during 1986-1990. Two experimental fields - Potrlica-I (600 m[sup 2]) and Potrlica-II (300 m[sup 2]) - were prepared by spreading a 10-20 cm thick layer of low-quality soil over the marly waste of the spoil bank. Soil composition was: 2.10% and 74% Ca, 4.5% and 0.70% humus, l3.5% and 5.5% P[sub 2]O[sub 5] and 23.0% and 6.0% K[sub 2]O in Potrlica-I and II respectively. Soil pH values were 7.2 and 7.60; 500 kg/ha NPK (15:15:15) were spread. Seventeen species of aromatic and medicinal plants were grown. After four years the plants were classified into 3 groups (thriving, satisfactory and non-thriving). It was found that the following plants can yield satisfactory crops and profits: valeriana officinalis, hyssopus officinalis, artemis dracunculus, cynaris scolimus, foenculum vulgare and malva silvestris. Unsatisfactory results were obtained with melisa officinalis, coriandrum sativum, pimpinela anisum and angelica archangelica. Experiments will be continued.

  19. Chemical and principal-component analyses of the essential oils of Apioideae taxa (Apiaceae) from central Balkan.

    Science.gov (United States)

    Kapetanos, Chrysostomos; Karioti, Anastasia; Bojović, Srdjan; Marin, Petar; Veljić, Milan; Skaltsa, Helen

    2008-01-01

    The volatile constituents of the essential oils of 23 taxa belonging to the Apioideae subfamily were studied in detail. The investigated taxa were Pimpinella serbica (Vis.) Bentham & Hooker, Libanotis montana Cr., Cnidium silaifolium (Jacq.) Simk. ssp. orientale (Boiss.) Tutin, Bupleurum praealtum L., B. sibthorpianum S. S. var. diversifolium (Roch.) Hay, Aegopodium podagraria L., Torilis anthriscus (L.) Gmel., Orlaya grandiflora (L.) Hoffm., Laserpitium siler L., Laser trilobum (L.) Brokh., Chaerophyllum aureum L., C. hirsutum L., C. temulum L., Pastinaca sativa L., P. hirsuta Pancic., Tordylium maximum L., Physospermum cornubiense (L.) DC., Peucedanum alsaticum L., P. oreoselinum (L.) Moench, P. cervaria (L.) Cuss., P. austriacum (Jacq.) Koch, P. longifolium W. et K., and P. officinale L. All of these species grow wild in the central part of the Balkan Peninsula. The essential oils were found to be complex mixtures of various compounds, more than 100 constituents being in each taxon, with contributions of main products never exceeding 25% of the total content. Sesquiterpene hydrocarbons were found to be the main group of constituents of all taxa, except for Peucedanum species, where monoterpene hydrocarbons were identified as the main components. The chemotaxonomic value of the essential-oil composition is discussed according to results of principal-component analysis (PCA). The essential-oil composition mainly reflects current taxonomic relationships between the investigated taxa.

  20. Vascular plant flora in the Cytadela cemeteries in Poznań (Poland

    Directory of Open Access Journals (Sweden)

    Aneta Czarna

    2016-12-01

    Full Text Available The paper presents the spontaneous vascular flora and the flora originating from old or contemporary plantations found in all six currently existing cemeteries located in immediate vicinity on the slopes of the Cytadela Park in Poznań. These studies were carried out in the years 2011–2014. Over this period, 255 species of vascular plants were found. The most interesting species include: Chionodoxa luciliae, Rumex rugosus, Aegopodium podagraria ‘Variegatum’, Ficaria verna f. plenifolia, Galanthus nivalis f. pleniflora, Ornithogalum boucheanum, Ranunculus repens ‘Plena’, and hybrids: Dactylis ×intercedens, Gagea ×pomeranica, Ornithogalum boucheanum × O. nutans, Viola cyanea × V. odorata. A great number of spring geophytes, namely 31 species, was also found. Among species occurring spontaneously outside the graves, some were new for Poland, e.g., Chionodoxa luciliae, Ornithogalum boucheanum × O. nutans, Viola cyanea × V. odorata, while others were new for the Wielkopolska region: Rumex rugosus, Dactylis ×intercedens, Gagea ×pomeranica, as well as new for Poznań: Erigeron ramosus, Lilium bulbiferum, Muscari armeniacus, M. neglectum, Pimpinella nigra, Poa subcaerulea, and Veronica hederifolia s. s.

  1. Phytochemicals and the breakthrough of traditional herbs in the management of sexual dysfunctions.

    Science.gov (United States)

    Adimoelja, A

    2000-01-01

    Traditional herbs have been a revolutionary breakthrough in the management of erectile dysfunction and have become known world-wide as an 'instant' treatment. The modern view of the management of erectile dysfunction subscribes to a single etiology, i.e. the mechanism of erection. A large number of pharmacological agents are orally consumed and vasoactive agents inserted intraurethrally or injected intrapenially to regain good erection. Modern phytochemicals have developed from traditional herbs. Phytochemicals focus their mechanism of healing action to the root cause, i.e. the inability to control the proper function of the whole body system. Hence phytochemicals manage erectile dysfunction in the frame of sexual dysfunction as a whole entity. Protodioscin is a phytochemical agent derived from Tribulus terrestris L plant, which has been clinically proven to improve sexual desire and enhance erection via the conversion of protodioscine to DHEA (De-Hydro-Epi-Androsterone). Preliminary observations suggest that Tribulus terrestris L grown on different soils does not consistently produce the active component Protodioscin. Further photochemical studies of many other herbal plants are needed to explain the inconsistent results found with other herbal plants, such as in diversities of Ginseng, Eurycoma longifolia, Pimpinella pruacen, Muara puama, Ginkgo biloba, Yohimbe etc.

  2. Approach to pharmacological and clinical applications of Anisi aetheroleum

    Institute of Scientific and Technical Information of China (English)

    Khaled; Mohamed; Mohamed; Koriem

    2015-01-01

    Anisi aetheroleum is the oil obtained from Pimpinella anisuin L.(P.anisuin) by steam distillation.P.anisuin seeds were air-dried,and then the dry seeds were crushed,pulverized,and weighed in sequence for anise oil preparation.P.anisuin is one of the oldest medicinal plants that belong to family Apiaceae.The fruit of P.anisuin is harvested in August and September.P.anisuin is widespread in Asia,Africa and Europe.Local names of P.anisuin include anise,anisoon,roomy,saunf,sweet cumin and yansoon.The anise oil odour is aromatic while the oil tastes sweet.The average daily dose of Anisi aetheroleum is 0.3 g.transAnethole is the major ingredient of the anise oil.Anisi aetheroleum also displays a protective action against neurotoxicity.In addition.Anisi aetheroleum increases glucose absorption and reduces urine output in the rat.The plant oil have pharmacological(antimicrobial,hepatoprotective.anticonvulsant,anti-inflammatory,antispasmodic,bronchodilator.estrogenic,expectorant and insecticidal) effects and clinical effects on nausea,constipation,menopausal period,virus,diabetes,obesity and sedative action.Owing to the wide application of Anisi aetheroleum in pharmacological and clinical fields,it is recommended for more clinical trails to discover a new medication from the active constituents of the plant oil in the future to treat human diseases especially chronic ones.

  3. 防治腹泻的植物组方

    Institute of Scientific and Technical Information of China (English)

    陈军(摘)

    2007-01-01

    一种饲料、食品或药品系由植物的叶、果实或根和适量香料和葡萄糖组成。其中含葡萄糖1%~20%(质量分数),植物成分1%~100%(质量分数)。植物可以是单一植物或由两种以上全植物粉末或它们的叶、果实、根或其水、醇提取物组成,如姜的根或其提取物、粗糙芹Trachyspermum amni的果实或其提取物、茴香果实或其提取物、胡椒果实或其提取物、洋茴芹Pimpinella anisum、姜黄根或其提取物、芒果果实、大蒜根、孜然芹果实或其提取物、儿茶Acacia catechu叶或其嫩枝提取物。

  4. Pesticide residues in leafy vegetables, stalk and stem vegetables from South Korea: a long-term study on safety and health risk assessment.

    Science.gov (United States)

    Park, Duck Woong; Kim, Kwang Gon; Choi, Eun Ah; Kang, Gyeong Ri; Kim, Tae Sun; Yang, Yong Shik; Moon, Su Jin; Ha, Dong Ryong; Kim, Eun Sun; Cho, Bae Sik

    2016-01-01

    South Korea has a unique food culture. South Koreans enjoy wrapping meat and eating or making kimchi (traditionally fermented Korean food) and eating using raw leafy vegetables, stalk and stem vegetables. Therefore, there is a high chance of being exposed to pesticide residues of vegetables. The objective of this study was to investigate pesticide residues in leafy vegetables, stalk and stem vegetables from South Korea. A total of 8496 samples were mainly collected from Gwangju and Jeonnam area (the largest production region of leafy vegetables, stalk and stem vegetables) in South Korea from 2010 to 2014. A total of 230 pesticides were used for multi-residue analysis of pesticides. Among 8496 samples, 61 different pesticides (1029 times) were detected in 890 samples, of which 118 samples (1.4%) exceeded the Korea maximum residue limits (MRLs). Samples exceeding the MRLs were mostly found in leafy vegetables (brassica lee ssp. namai, leafy lettuce, spinach, perilla leaves, crown daisy, marsh mallow, aster scaber, pimpinella brachycarpa) and Chinese chive. Procymidone, dimethomorph and azoxystrobin were the most frequently found pesticides. A risk assessment of pesticides exceeding the MRLs was evaluated by calculating the estimated daily intake (EDI) and the acceptable daily intake (ADI). The ratio of EDI to ADI was 0.003-30.4%.

  5. Impact of aqueous extracts from six flavoring materials on rats' gut Lactobacilli%六种香辛料水提取物对大鼠肠道乳酸菌影响的研究

    Institute of Scientific and Technical Information of China (English)

    张志超; 黄丽珠; 王聪; 张宁; 刘柳; 彭喜春

    2013-01-01

    Objective: Six flavoring materials of Cinnamomum cassia, Zingiber officinale Rose, Syzygium aromatieum, Foeniculum vulgare Mill, Piper nigrum L. and Zanthoxylum bungeanum were examined whether to have positive effects on dysfunction of digestive system such as gastrointestinal dysfunction and diarrhea. Methods: The amount, diversity and composition of Lactobacilli in rat colon and rectum were analyzed with Ribotype abundance ( S) , Shannon diversity index ( H) , Evenness index and Jacaard similarity index by using the technique of terminal- restriction fragment length polymorphism after the aqueous extracts of six materials were orally administrated into rats for one month. Results : The results showed that the aqueous extracts of six materials had different effects on Lactobaciili in rat colon,but presented to inhibit them in rectum. However, all of these extracts enhanced the diversity and distribution evenness of Lactobacilli in colon except for that of Syzygium aromatieum and Foeniculum vulg are Mill. Foeniculum vulgare Mill reduced the diversity and distribution evenness of Lactobacilli in rectum. Conclusion : These extracts exerted a heavy impact on the composition of Lactobacilli in both colon and rectum. The functional mechanism of these materials on the dysfunction of digestive system might be relative with the shared and different effects on gut Lactobacilli.%目的:观察肉桂、干姜、丁香、小茴香、胡椒和花椒等六种香辛料水提取物对消化系统功能紊乱的积极作用.方法:本研究采用末端限制性片段长度多态性分析技术,再用总峰面积、Ribotype丰富度(S)、Shannon多样性指数(H)、均匀度指数(E)以及Jacaard相似性指数等指标,针对六种常见香辛料的水提取物对大鼠结肠和直肠中乳酸菌群的数量、多样性及结构进行了分析. 结果:六种香辛料的水提取物对结肠中乳酸菌群生长的影响存在差异,但均抑制了直肠中乳酸菌的生长;然

  6. Study on the Repellent Activity of Plant Essectial Oils Against Latheticus oryzae Waterhouse%5种植物精油对长头谷盗的控制作用研究

    Institute of Scientific and Technical Information of China (English)

    郭钰; 曾玲; 梁广文

    2013-01-01

    选取小茴香、肉桂、丁香、辣椒和生姜精油,研究其对长头谷盗的控制作用.结果表明:(1)5种精油对长头谷盗均具有显著的驱避活性,其中丁香和生姜精油的5个试验浓度(1∶1、1∶10、1∶15、1∶20和1∶50)对长头谷盗的驱避效果最好,处理60h后,其驱避活性均达到Ⅴ级,72 h后,其驱避活性分别仍达到Ⅲ级和Ⅳ级;(2)小茴香、肉桂和丁香精油对长头谷盗有明显的触杀作用,其中处理最低浓度(1∶50)的肉桂时,长头谷盗的死亡率仍在60%以上;辣椒和生姜精油的触杀活性较弱,5个试验浓度对长头谷盗的死亡率几乎为零;(3)5种精油对长头谷盗均无明显的熏蒸作用.%Five plant essential oils of Foeniculum vulgare,Cinnamomum cassia,Syzygium aromaticum,Capsicum annuum and Zingiber officinale were used to investigate their repellent action,contact action and fumigation against Latheticas oryzae Waterhouse in this paper.The results showed that:(1) Five plant essential oils significantly repelled L.oryzae,and Syzygium aromaticum and Zingiber officinale with 5 tests concentration(1∶1,1 ∶ 10,1∶15,1∶20 and 1 ∶ 50) had the best repellent effects.60 h later,the repellency percentages was Ⅴ level,and 72 h later,the percentages were Ⅲ level and Ⅳ level,respectively.(2)Foeniculum vulgare,Cinnamomum cassia and Syzygium aromaticum essential oils could effectivelf kill L.oryzae adults.The contact toxicity of Foeniculum vulgare essential oil at 1∶ 50 concentration had no contact effect to L.oryzae,but the mortality of 1 ∶ 50 concentration was over 60%.However,the contact activity of Capsicum annuum and Zingiber officinale essential oils were weak,and the mortality of L.oryzae of five test concentrations were nearly zero.(3) In a word,all of the five kinds of essential oils couldn' t effectively fumigate L.oryzae adults.

  7. Estudo da concordância das citações de uso e importância das espécies e famílias utilizadas como medicinais pela comunidade do bairro Ponta Grossa, Porto Alegre, RS, Brasil

    Directory of Open Access Journals (Sweden)

    Giovana Secretti Vendruscolo

    2006-06-01

    Full Text Available Ferramentas para quantificar dados etnobotânicos estão sendo usadas como complementares aos levantamentos sobre a utilização de plantas por populações. Neste trabalho são utilizadas técnicas para avaliar a concordância das citações de uso e a importância das espécies e famílias para as 51 pessoas entrevistadas no bairro Ponta Grossa, Porto Alegre, Rio Grande do Sul. Para tal, foram utilizados os cálculos de Valor de Uso (UV e a porcentagem corrigida de Concordância quanto aos Usos Principais (CUPc para as 142 espécies mencionadas no levantamento. As espécies Aloe arborescens Mill., Citrus × aurantium L., Achyrocline satureioides (Lam. DC., Foeniculum vulgare Mill, Eugenia uniflora L., Cunila microcephala Benth., Citrus limon (L. Osveck, Plectranthus barbatus Andrews, Cymbopogon citratus (DC. Stapf, Psidium guajava L., Artemisia absinthium L., Ocimum basilicum L., Plantago tomentosa Lam., Rosmarinus officinalis L., Persea americana Mill., Aloysia citrodora Palau, Sambucus australis Cham. & Schltdl., Cuphea carthagenensis (Jacq. J.F. Macbr., Petroselinum crispum (Mill. Nyman ex A.W. Hill, Ocimum selloi Benth. e Tanacetum vulgare L., nesta ordem de Valor de Uso, foram consideradas como as mais importantes para a população estudada. As famílias mais importantes foram Asphodelaceae, Caprifoliaceae, Rutaceae e Lythraceae. Para o cálculo da porcentagem a corrigida de Concordância quanto aos Usos Principais (CUPc foram consideradas como espécies principais as que apresentaram valores acima de 24%: Eugenia uniflora, Achyrocline satureioides, Psidium guajava, Cunila microcephala, Plectranthus barbatus, Citrus × aurantium, Citrus limon, Cymbopogon citratus, Punica granatum L., Sechium edule (Jacq. Sw., Sphagneticola trilobata (L. Pruski, Aloysia citrodora, Foeniculum vulgare, Plectranthus neochilus Schltr., Artemisia absinthium, Lippia alba (Mill. N.E. Br., Mikania laevigata Sch. Bip ex Baker, Aloe arborescens e Petroselinum

  8. 八种药用植物精油对谷蠹成虫的熏蒸活性%Fumigant activities of eight essential oils of herb plants against Rhizopertha dominica(Fab.)adults

    Institute of Scientific and Technical Information of China (English)

    黄衍章; 李世广; 操海群; 巢道平; 周倩

    2011-01-01

    The fumigant activities of eight essential oils of herb plants against adults of Rhizopertha dominica (Fab.) were tested in the sealed triangular flask. The result indicated that Illicium verum, Foeniculum vulgare and Acorus gramineus oils were possessed of good fumigant activities against the tested pests, especially the Illicium verum oils. Under the condition of empty-storehouse-imitation, the Illicium verum and Foeniculum vulgare oils showed excellent fumigant activities against the tested pests, and the LC50 to R. Dominica (Fab.) after treatment for 3 days were 2.88 uL-L"1 and 4.02 μL·L-1, respectively. Of all eight herb plants essential oils, only Illicium verum showed good fumigant activities under the condition of real-storehouse-imitation, and the oil produced 92.37% corrected mortalities of pests at 166.67 μL·L-1 after treatment for 10 days. Further bioassay showed the LC50 of Illicium verum oil against R. Dominica (Fab.) after treatment for 14 days under the condition of real-storehouse-imitation was 67.01 uL·L-1-1.%采用三角瓶密闭熏蒸法,测试了8种药用植物精油在空仓和实仓模拟条件下对谷蠹成虫的熏蒸活性.结果表明,8种植物精油以八角茴香,小茴香和石菖蒲时试虫的熏杀效果较好,尤以八角茴香精油的熏杀效果最佳.空仓模拟条件下,八角茴香和小茴香精油均对谷蠹成虫表现出很高的熏杀活性,其熏蒸处理3d后的LC50分别为2.88 μL·L-1和4.02 μL·L-1.实仓模拟条件下,8种植物精油仅以八角茴香的熏蒸效果较好,其166.67 μL·L-1熏蒸处理10d后试虫的校正死亡率为92.37%,实仓模拟熏蒸处理14d后的LC50为67.01 μL·L-1.

  9. The Selenium Content in 18 Kinds of Edible Spices%18种食用香料中的硒含量特征

    Institute of Scientific and Technical Information of China (English)

    苟体忠; 张文华; 唐文华; 蒋天智; 孙大方; 彭章美; 毛仕焦; 梁文涛

    2014-01-01

    研究食用香料中硒含量,以便为富硒食用香料的选择提供重要参考。采用氢化物发生-原子荧光光谱法测定18种食用香料中的硒含量。结果表明:18种食用香料中的硒含量变化较大,其含量变化在0.002~0.849 mg/kg之间。千里香、小茴香、香果、孜然、鱼香菜和鱼蓼中的硒含量较高,其含量分别为0.849,0.719,0.669,0.560,0.435,0.149 mg/kg。分析认为千里香、小茴香、香果、孜然、鱼香菜和鱼蓼已达到富硒调味品标准。%To study the selenium content in edible spices,which may provide important reference for picking selenium-enriched edible spices.The content of selenium in 18 kinds of edible spices is analyzed by hydride generation-atomic fluorescence spectrometry.The results show that there are major changes in the selenium content in 18 kinds of edible spices,ranging from 0.002 mg/kg to 0.849 mg/kg.Murraya paniculata (L.)Jack.,Foeniculum vulgare Mill.,Ligusticum chuanxiong Hort.,Cuminum cyminum L.,Mentha spicata Linn.and Polygonum lapathifolium L.have higher selenium content,which is 0.849,0.719,0.669,0.560,0.435,0.149 mg/kg.Analysts believe that Murraya paniculata(L.)Jack.,Foeniculum vulgare Mill.,Ligusticum chuanxiong Hort.,Cuminum cyminum L.,Mentha spicata Linn.and Polygonum lapathifolium L.have reached the standard of selenium-enriched condiment.

  10. [Antioxidant properties of essential oils].

    Science.gov (United States)

    Misharina, T A; Terenina, M B; Krikunova, N I

    2009-01-01

    By the method of capillary gas-liquid chromatography we studied antioxidant properties and stability during the storage of hexane solutions of 14 individual essential oils from black and white pepper (Piper nigrum L.), cardamom (Elettaria cardamomum L.), nutmeg (Myristica fragrans Houtt.), mace (Myristica fragrans Houtt), juniperberry (Juniperus communis L.), seed of fennel (Foeniculum vulgare Mill., var. dulce Thelling), caraway (Carvum carvi L.), dry leaves of cinnamon (Cinnamomum zeylanicum Bl.), marjoram (Origanum majorana L.), laurel (Laurus nobilis L.), ginger (Zingiber officinale L.), garlic (Allium sativum L.), and clove bud (Caryophyllus aromaticus L.). We assessed the antioxidant properties by the oxidation of aliphatic aldehyde (trans-2-hexenal) into the according carbon acid. We established that essential oils of garlic, clove bud, ginger and leaves of cinnamon have the maximal efficiency of inhibition of hexenal oxidation (80-93%), while black pepper oil has the minimal (49%). Antioxidant properties of essential oils with a high content of substituted phenols depended poorly on its concentration in model systems. We studied the changes in essential oils content during the storage of its hexane solutions for 40 days in the light and out of the light and compared it with the stability of essential oils stored for a year out of the light.

  11. Effect of essential oils in control of plant diseases.

    Science.gov (United States)

    Peighami-Ashnaei, S; Farzaneh, M; Sharifi-Tehrani, A; Behboudi, K

    2009-01-01

    In this study, antifungal activity of some essential oils, extracted from Syzygium aromoticum, Foeniculum vulgare, Cuminum cyminum and Mentha piperita were investigated against grey mould of apple. The essential oils of S. aromaticum and F. vulgare showed considerable antifungal activities on PDA medium against Botrytis cinerea. Results indicated that the increasing of dosage of the essential oils caused to the more antifungal activity against B. cinerea in vitro condition. After 10 days, results showed that the essential oil of F. vulgare in both of the concentrations (750 and 1000 microL/L) was more effective than the essential oil of S. aromaticum against grey mould of apple and decrease the disease up to 15.5% in comparison with the check treatment (100%). After 20 days, biocontrol potential of the essential oils of S. aromaticum and F. vulgare at 1000 microL/L were more effective than the other treatments and the percentage of disease was evaluated 41.6% and 50.8%, respectively, in comparison with the check treatment (100%).

  12. An overview of amenorrhea and respective remedies in Traditional Persian Medicine

    Directory of Open Access Journals (Sweden)

    Abdolmajid Elahi

    2016-03-01

    Full Text Available Amenorrhea is described as complete absence or cessation of menstruation. Besides conventional treatment approaches, traditional and complementary medical systems have suggested numerous natural medications for management of amenorrhea. Current study aimed to compile the pharmacological reports on amenorrhea from the standpoints of early Persian scholars. Medical and pharmaceutical manuscripts of Persian medicine from 9th to 18th centuries A.D. have been reviewed to extract the critical points and natural remedies. Based on Traditional Persian Medicine (TPM, impairment in uterus, brain and circulation are main causes of amenorrhea. Any obstruction, either anatomical or functional, in uterus and allied organs or tissues may lead the body to this disorder. Concerning herbal therapy of amenorrhea in TPM, 71 medicinal plants related to 35 families were found. The most prevalent families were Apiaceae, Asteraceae, Lamiaceae and Fabaceae, respectively. Usual routes of administration were oral, vaginal and topical. However, there was one report on a medicament, spoken to be effective nasally. Traditionally, emmenagogue medicines should possess diuretic activity to be effective for amenorrhea. All reported remedies were known as diuretic agents. However, this description is not corresponded with what is now accepted as diuretic. On the other hand, only Foeniculum vulgare showed therapeutic effects on amenorrhea in a randomized placebo-controlled trial. Despite lack of novel information on emmenagogue activity of these remedies, design and conducting evidence-based animal or human studies may be beneficial for new drug discovery from traditional knowledge.

  13. Effects of Herbal Essential Oil Mixture as a Dietary Supplement on Egg Production in Quail

    Directory of Open Access Journals (Sweden)

    Metin Çabuk

    2014-01-01

    Full Text Available One hundred and eighty 7-week-old laying quail were fed various diets over a 12-week period. The diets included a control diet (without essential oil mixture (EOM or antibiotics (ANTs, a basal diet including EOM (24 mg/kg feed, and a basal diet including an ANT (avilamycin, 10 mg/kg feed. Each treatment comprised 4 replications with 4 cages (15 quail per cage, amounting to 60 quail per treatment group. Diets (in mash form and water were provided for ad libitum consumption. EOM consisted of 6 different essential oils derived from the following herbs: oregano (Origanum sp., laurel leaf (Laurus nobilis L., sage leaf (Salvia triloba L., myrtle leaf (Myrtus communis, fennel seeds (Foeniculum vulgare, and citrus peel (Citrus sp.. In comparison with the control diet, adding supplements such as EOM and ANTs to the basal diet increased egg production in quail (P<0.001. However, egg production was similar between EOM and ANT treatment groups. Moreover, there were no differences between the treatment groups with regard to egg weight. Feed intake was not affected by EOM or ANT supplementation, whereas feed conversion ratio was significantly improved by EOM and ANT supplementation. Thus, we concluded that EOM has beneficial effects as a dietary supplement on egg production and feed conversion ratio.

  14. Use of Medicinal Plants of District Bannu in Unani Medicines

    Directory of Open Access Journals (Sweden)

    Shahzeb

    2013-06-01

    Full Text Available The present research work was carried out during 2012 in district Bannu to study the use of medicinal plants in Unani medicine. A total of 35 Unani medicines were reported and arranged systematically along with name of product, available form, company name, name of the plants/parts used in the drugs, family name and purpose of uses. During the research it was found that most of the members of Lamiaceae, Asteraceae and Rhamnaceae are used in these medicines. Plants which were used commonly in these medicines in one form or the other are Ziziphus jujuba, Foeniculum vulgare, Solanum nigrum, Ocimum cannum and Zingber officinale. It was noted that these products are mostly available in syrup form, rarely in tablets form (Hab –khoom Safa, Mensorine, Scony Tablets and one only one product in dry powder (Supari Pak. It is generally believed that these medicines have no side effect. Interestingly one medicine is suggested for many diseases as per the given instructions in the medicine pack. It was also noted that the manufacturers of these medicines are mostly not registered that’s why incomplete addresses along with the wrongly spellings plant name given on the medicine packs. The main purpose of the research was to identify and enlist the plants systematically used in these medicines.

  15. Evaluation of native plant flower characteristics for conservation biological control of Prays oleae.

    Science.gov (United States)

    Nave, A; Gonçalves, F; Crespí, A L; Campos, M; Torres, L

    2016-04-01

    Several studies have shown that manipulating flowering weeds within an agroecosystem can have an important role in pest control by natural enemies, by providing them nectar and pollen, which are significant sources of nutrition for adults. The aim of this study was to assess if the olive moth, Prays oleae (Bernard, 1788) (Lepidoptera: Praydidae), and five of its main natural enemies, the parasitoid species Chelonus elaeaphilus Silvestri (Hymenoptera: Braconidae), Apanteles xanthostigma (Haliday) (Hymenoptera: Braconidae), Ageniaspis fuscicollis (Dalman) (Hymenoptera: Encyrtidae) and Elasmus flabellatus (Fonscolombe) (Hymenoptera: Eulophidae), as well as the predator Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae), can theoretically access the nectar from 21 flowering weeds that naturally occur in olive groves. Thus, the architecture of the flowers as well as the mouthpart structure and/or the head and thorax width of the pest and its enemies were analyzed. The results suggested that all beneficial insects were able to reach nectar of the plant species from Apiaceae family, i.e. Conopodium majus (Gouan) Loret, Daucus carota L. and Foeniculum vulgare Mill., as well as Asparagus acutifolius L., Echium plantagineum L., Capsella bursa-pastoris (L.) Medik., Raphanus raphanistrum L., Lonicera hispanica Boiss. et Reut., Silene gallica L., Spergula arvensis L., Hypericum perforatum L., Calamintha baetica Boiss. et Reut, Malva neglecta Wallr. and Linaria saxatilis (L.) Chaz. P. oleae was not able to access nectar from five plant species, namely: Andryala integrifolia L., Chondrilla juncea L., Dittrichia viscosa (L.) Greuter, Sonchus asper (L.) Hill and Lavandula stoechas L.

  16. Extraction of volatile oil from aromatic plants with supercritical carbon dioxide: experiments and modeling.

    Science.gov (United States)

    Coelho, Jose P; Cristino, Ana F; Matos, Patrícia G; Rauter, Amélia P; Nobre, Beatriz P; Mendes, Rui L; Barroso, João G; Mainar, Ana; Urieta, Jose S; Fareleira, João M N A; Sovová, Helena; Palavra, António F

    2012-09-05

    An overview of the studies carried out in our laboratories on supercritical fluid extraction (SFE) of volatile oils from seven aromatic plants: pennyroyal (Mentha pulegium L.), fennel seeds (Foeniculum vulgare Mill.), coriander (Coriandrum sativum L.), savory (Satureja fruticosa Béguinot), winter savory (Satureja montana L.), cotton lavender (Santolina chamaecyparisus) and thyme (Thymus vulgaris), is presented. A flow apparatus with a 1 L extractor and two 0.27 L separators was built to perform studies at temperatures ranging from 298 to 353 K and pressures up to 30.0 MPa. The best compromise between yield and composition compared with hydrodistillation (HD) was achieved selecting the optimum experimental conditions of extraction and fractionation. The major differences between HD and SFE oils is the presence of a small percentage of cuticular waxes and the relative amount of thymoquinone, an oxygenated monoterpene with important biological properties, which is present in the oils from thyme and winter savory. On the other hand, the modeling of our data on supercritical extraction of volatile oil from pennyroyal is discussed using Sovová's models. These models have been applied successfully to the other volatile oil extractions. Furthermore, other experimental studies involving supercritical CO(2) carried out in our laboratories are also mentioned.

  17. Uso de Plantas Medicinais por Famílias do Vale do Jequitinhonha, Minas Gerais, Brasil

    Directory of Open Access Journals (Sweden)

    Maria Jesus Barreto CRUZ

    2015-01-01

    Full Text Available O objetivo do estudo foi investigar a utilização de plantas medicinais pelas famílias residentes no Vale do Jequitinhonha, Minas Gerais. Foi realizado um estudo descritivo tipo inquérito populacional domiciliar, amostra constituída por 555 domicílios selecionados de maneira aleatória simples por meio de amostragem por conglomerado de 137 setores censitários. Dos participantes, 73,51% relataram a utilização de plantas medicinais, sendo Mentha x piperita L., Mentha pulegium L. e Foeniculum vulgare Mill as mais citadas. Dos que utilizaram plantas medicinais, 48% preparavam por decocção, 46,8% por infusão, 77% aprenderam com os pais como utilizar e preparar. Foi observado que 90,7% obtiveram as plantas dos próprios quintais, 90,9% consumiam frescas e 94,4% achavam que não fazia mal. A utiliza- ção de plantas medicinais é comum nos municípios estudados. Assim, nota-se a necessidade de realização de pesquisa etnobotânica e capacitação dos profissionais de saúde para promover o uso racional de plantas medicinais.

  18. Antimycotic activities of selected plant flora, growing wild in Lebanon, against phytopathogenic fungi.

    Science.gov (United States)

    Abou-Jawdah, Yusuf; Sobh, Hana; Salameh, Abdu

    2002-05-22

    Petroleum ether (PE) and methanolic extracts of nine wild plant species were tested in vitro for their antimycotic activity against eight phytopathogenic fungi. The efficacy of PE extracts against all pathogens tested was higher than that of methanolic extracts. Wild marjoram (Origanum syriacum) PE extract showed the highest and widest range of activity. It resulted in complete inhibition of mycelial growth of six of eight fungi tested and also gave nearly complete inhibition of spore germination of the six fungi included in the assay, namely, Botrytis cinerea, Alternaria solani, Penicillium sp., Cladosporium sp., Fusarium oxysporum f. sp. melonis, and Verticillium dahlia. The other plant extracts showed differential activities in the spore germination test, but none was highly active against mycelial growth. Inula viscosa and Mentha longifolia were highly effective (>88%) in spore germination tests against five of six fungi tested, whereas Centaurea pallescens, Cichorium intybus, Eryngium creticum, Salvia fruticosa, and Melia azedarach showed >95% inhibition of spore germination in at least two fungi. Foeniculum vulgare showed the least antimycotic activity. Fractionation followed by autobiography on TLC plates using Cladosporium sp. as a test organism showed that O. syriacum PE extracts contained three inhibition zones, and those of Inula viscosa and Cichorium intybus, two, whereas the PE extracts of the remaining plants showed each one inhibition zone. Some of the major compounds present in these inhibition zones were identified by GC-MS. The possibility for using these extracts, or their mixtures, to control plant diseases is discussed.

  19. Activity against drug resistant-tuberculosis strains of plants used in Mexican traditional medicine to treat tuberculosis and other respiratory diseases.

    Science.gov (United States)

    Camacho-Corona, María Del Rayo; Ramírez-Cabrera, Mónica A; Santiago, Omar González-; Garza-González, Elvira; Palacios, Isidoro de Paz; Luna-Herrera, Julieta

    2008-01-01

    Tuberculosis (TB) kills about 3 million people per year worldwide. Furthermore, TB is an infectious disease associated with HIV patients, and there is a rise in multidrug-resistant TB (MDR-TB) cases around the world. There is a need for new anti-TB agents. The study evaluated the antimycobacterial activity of nine plants used in Mexican traditional medicine to treat tuberculosis and other respiratory diseases. Nasturtium officinale showed the best activity (MIC = 100 microg/mL) against the sensitive Mycobacterium tuberculosis. The following plants were active also but at 200 microg/mL: Citrus sinensis, Citrus aurantifolia, Foeniculum vulgare, Larrea tridentata, Musa acuminata and Olea europaea. Contrary to the above data, activity against drug-resistant variants of M. tuberculosis was more evident, e.g. N. officinale was the most potent (MIC aurantifolia, C. sinensis and O. europaea (MIC = 25 microg/mL). These data point to the importance of biological testing of extracts against drug-resistant M. tuberculosis isolates, and the bioguided assay of these extracts for the identification of lead compounds against MDR-TB isolates. Copyright (c) 2007 John Wiley & Sons, Ltd.

  20. Anti-Aging Potential of Phytoextract Loaded-Pharmaceutical Creams for Human Skin Cell Longetivity

    Science.gov (United States)

    Jadoon, Saima; Karim, Sabiha; Asad, Muhammad Hassham Hassan Bin; Akram, Muhammad Rouf; Kalsoom Khan, Abida; Malik, Arif; Chen, Chunye; Murtaza, Ghulam

    2015-01-01

    The exposure to ultraviolet radiations (UVR) is the key source of skin sunburn; it may produce harmful entities, reactive oxygen species (ROS), leading to aging. The skin can be treated and protected from the injurious effects of ROS by using various pharmaceutical formulations, such as cream. Cream can be loaded with antioxidants to quench ROS leading to photo-protective effects. Moreover, modern medicines depend on ethnobotanicals for protection or treatment of human diseases. This review article summarizes various in vivo antioxidant studies on herbal creams loaded with phyto-extracts. These formulations may serve as cosmeceuticals to protect skin against injurious effects of UVR. The botanicals studied for dermatologic use in cream form include Acacia nilotica, Benincasa hispida, Calendula officinalis, Camellia sinensis, Camellia sinensis, Nelumbo nucifera, Capparis decidua, Castanea sativa, Coffea arabica, Crocus sativus, Emblica officinalis Gaertn, Foeniculum vulgare, Hippophae rhamnoides, Lithospermum erythrorhizon, Malus domestica, Matricaria chamomilla L., Moringa oleifera, Morus alba, Ocimum basilicum, Oryza sativa, Polygonum minus, Punica granatum, Silybum marianum, Tagetes erecta Linn., Terminalia chebula, Trigonella foenum-graecum, and Vitis vinifera. The observed anti-aging effects of cream formulations could be an outcome of a coordinating action of multiple constituents. Of numerous botanicals, the phenolic acids and flavonoids appear effective against UVR-induced damage; however the evidence-based studies for their anti-aging effects are still needed. PMID:26448818

  1. Comparative Study on the Adaptation and Growth Dynamics of the Helix pomatia and Helix aspersa Muller Terrestrial Snails Under Different Feeding Regimes

    Directory of Open Access Journals (Sweden)

    Adrian Toader-Williams

    2010-05-01

    Full Text Available We used Helix pomatia and Helix aspersa species and measure their growth as the snails were approaching the hibernation season. Helix pomatia 2yo shown a decrease in weight while being raised in enclosed parcels of 4sqm the younger Helix pomatia 1yo as well as Helix aspersa Muller demonstrated the ability to adapt relatively fast to the same conditions. We established 5 experimental lots in a Helix pomatia farm, GPS coordinates N46.606040 E23.599950. Control lot contained Taraxacum officinales, Sonchus oleraceus, Equisetum arvense and Atriplex hortensis, wild flora found within the farm. The other lots contained the same plants as the control lot plus different combinations of imported plants from other areals. The H. pomatia 2yo weight decreased in the control lot by a mean of -3.86% while H. aspersa 1yo marked an increase of +16.89% in the same lot during the same period. The lot containing lupinus polyphyllus delivered snails with weight gain of +24.66% for H. pomatia 2yo and an increase of only +1.98% for H. aspersa 1yo. As a contrast, H. pomatia 2yo gained only +7.72% while H. aspersa 1yo gained +28.89%, in the lot containing Lavanda officinalis, Foeniculum vulgare and Hyssopus officinalis among the other plants.

  2. Extraction of Volatile Oil from Aromatic Plants with Supercritical Carbon Dioxide: Experiments and Modeling

    Directory of Open Access Journals (Sweden)

    Helena Sovová

    2012-09-01

    Full Text Available An overview of the studies carried out in our laboratories on supercritical fluid extraction (SFE of volatile oils from seven aromatic plants: pennyroyal (Mentha pulegium L., fennel seeds (Foeniculum vulgare Mill., coriander (Coriandrum sativum L., savory (Satureja fruticosa Béguinot, winter savory (Satureja montana L., cotton lavender (Santolina chamaecyparisus and thyme (Thymus vulgaris, is presented. A flow apparatus with a 1 L extractor and two 0.27 L separators was built to perform studies at temperatures ranging from 298 to 353 K and pressures up to 30.0 MPa. The best compromise between yield and composition compared with hydrodistillation (HD was achieved selecting the optimum experimental conditions of extraction and fractionation. The major differences between HD and SFE oils is the presence of a small percentage of cuticular waxes and the relative amount of thymoquinone, an oxygenated monoterpene with important biological properties, which is present in the oils from thyme and winter savory. On the other hand, the modeling of our data on supercritical extraction of volatile oil from pennyroyal is discussed using Sovová’s models. These models have been applied successfully to the other volatile oil extractions. Furthermore, other experimental studies involving supercritical CO2 carried out in our laboratories are also mentioned.

  3. Biodegradable gelatin-chitosan films incorporated with essential oils as antimicrobial agents for fish preservation.

    Science.gov (United States)

    Gómez-Estaca, J; López de Lacey, A; López-Caballero, M E; Gómez-Guillén, M C; Montero, P

    2010-10-01

    Essential oils of clove (Syzygium aromaticum L.), fennel (Foeniculum vulgare Miller), cypress (Cupressus sempervirens L.), lavender (Lavandula angustifolia), thyme (Thymus vulgaris L.), herb-of-the-cross (Verbena officinalis L.), pine (Pinus sylvestris) and rosemary (Rosmarinus officinalis) were tested for their antimicrobial activity on 18 genera of bacteria, which included some important food pathogen and spoilage bacteria. Clove essential oil showed the highest inhibitory effect, followed by rosemary and lavender. In an attempt to evaluate the usefulness of these essential oils as food preservatives, they were also tested on an extract made of fish, where clove and thyme essential oils were the most effective. Then, gelatin-chitosan-based edible films incorporated with clove essential oil were elaborated and their antimicrobial activity tested against six selected microorganisms: Pseudomonas fluorescens, Shewanella putrefaciens, Photobacterium phosphoreum, Listeria innocua, Escherichia coli and Lactobacillus acidophilus. The clove-containing films inhibited all these microorganisms irrespectively of the film matrix or type of microorganism. In a further experiment, when the complex gelatin-chitosan film incorporating clove essential oil was applied to fish during chilled storage, the growth of microorganisms was drastically reduced in gram-negative bacteria, especially enterobacteria, while lactic acid bacteria remained practically constant for much of the storage period. The effect on the microorganisms during this period was in accordance with biochemical indexes of quality, indicating the viability of these films for fish preservation. 2010 Elsevier Ltd. All rights reserved.

  4. Evaluation of antifungal activity of essential oils against potentially mycotoxigenic Aspergillus flavus and Aspergillus parasiticus

    Directory of Open Access Journals (Sweden)

    Fernanda C. da Silva

    2012-10-01

    Full Text Available The antifungal activity of essential oils of fennel (Foeniculum vulgare Mill., Apiaceae, ginger (Zingiber officinale Roscoe, Zingiberaceae, mint (Mentha piperita L., Lamiaceae and thyme (Thymus vulgaris L., Lamiaceae was evaluated against mycotoxin producers Aspergillus flavus and A. parasiticus. High Resolution Gas Chromatography was applied to analyze chemical constituents of essential oils. The effect of different concentrations of essential oils was determined by solid medium diffusion assay. Mycelial growth and sporulation were determined for each essential oil at the concentrations established by solid medium diffusion assay. At the fifth, seventh and ninth days the mycelial diameter (Ø mm and spore production were also determined. FUN-1 staining was performed to assess cell viability after broth macrodilution assay. Trans-anethole, zingiberene, menthol and thymol are the major component of essential oils of fennel, ginger, mint and thyme, respectively. The effective concentrations for fennel, ginger, mint and thyme were 50, 80, 50 and 50% (oil/DMSO; v/v, respectively. The four essential oils analysed in this study showed antifungal effect. Additionally, FUN-1 staining showed to be a suitable method to evaluate cell viability of potential mycotoxigenic fungi A. flavus and A. parasiticus after treatment with essential oils.

  5. Can Estragole in Fennel Seed Decoctions Really Be Considered a Danger for Human Health? A Fennel Safety Update

    Directory of Open Access Journals (Sweden)

    L. Gori

    2012-01-01

    Full Text Available Fennel (Foeniculum vulgare Mill. mature fruit (commonly known as seeds and essential oil of fennel are widely used as flavoring agents in food products such as liqueurs, bread, cheese, and an ingredient of cosmetics and pharmaceutical products. Moreover fennel infusions are the classical decoction for nursing babies to prevent flatulence and colic spasm. Traditionally in Europe and Mediterranean areas fennel is used as antispasmodic, diuretic, anti-inflammatory, analgesic, secretomotor, secretolytic, galactagogue, eye lotion, and antioxidant remedy and integrator. Topically, fennel powder is used as a poultice for snake bites. In Asian cultures fennel was ingested to speed the elimination of poisons. As one of the ancient Saxon people's nine sacred herbs, fennel was credited with the power to cure. Fennel was also valued as a magic herb: in the Middle Ages it was draped over doorways on Midsummer's Eve to protect the household from evil spirits. Recently because of estragole carcinogenicity, fennel has been charged to be dangerous for humans especially if used as decoction for babies. But this allegation do not consider the remedy is prepared as a matrix of substances, and recent researches confirm that pure estragole is inactivated by many substance contained in the decoction.

  6. Can estragole in fennel seed decoctions really be considered a danger for human health? A fennel safety update.

    Science.gov (United States)

    Gori, L; Gallo, E; Mascherini, V; Mugelli, A; Vannacci, A; Firenzuoli, F

    2012-01-01

    Fennel (Foeniculum vulgare Mill.) mature fruit (commonly known as seeds) and essential oil of fennel are widely used as flavoring agents in food products such as liqueurs, bread, cheese, and an ingredient of cosmetics and pharmaceutical products. Moreover fennel infusions are the classical decoction for nursing babies to prevent flatulence and colic spasm. Traditionally in Europe and Mediterranean areas fennel is used as antispasmodic, diuretic, anti-inflammatory, analgesic, secretomotor, secretolytic, galactagogue, eye lotion, and antioxidant remedy and integrator. Topically, fennel powder is used as a poultice for snake bites. In Asian cultures fennel was ingested to speed the elimination of poisons. As one of the ancient Saxon people's nine sacred herbs, fennel was credited with the power to cure. Fennel was also valued as a magic herb: in the Middle Ages it was draped over doorways on Midsummer's Eve to protect the household from evil spirits. Recently because of estragole carcinogenicity, fennel has been charged to be dangerous for humans especially if used as decoction for babies. But this allegation do not consider the remedy is prepared as a matrix of substances, and recent researches confirm that pure estragole is inactivated by many substance contained in the decoction.

  7. Chemical Composition, Antioxidant and Antimicrobial Activity of Essential Oils from Organic Fennel, Parsley, and Lavender from Spain

    Science.gov (United States)

    Marín, Irene; Sayas-Barberá, Estrella; Viuda-Martos, Manuel; Navarro, Casilda; Sendra, Esther

    2016-01-01

    The aim of this work was to (i) determine the chemical composition of the essential oils of three spices widely cultivated in Spain from organic growth: Foeniculum vulgare, Petroselium crispum, and Lavandula officinalis; (ii) determine the total phenolic content; (iii) determine the antioxidant activity of the essentials oils by means of three different antioxidant tests and (iv) determine the effectiveness of these essentials oils on the inhibition of Listeria innocua CECT 910 and Pseudomonas fluorescens CECT 844. There is a great variability in the chemical composition of the essential oils. Parsley had the highest phenolic content. Overall, parsley presented the best antioxidant profile, given its highest % of inhibition of DPPH radical (64.28%) and FRAP (0.93 mmol/L Trolox), but had a pro-oxidative behavior by TBARS. Lavender essential oil showed the highest antibacterial activity against L. innocua (>13 mm of inhibition at 20–40 μL oil in the discs), followed by parsley with an inhibition zone of 10 mm (when more than 5 μL oil in the discs), and fennel 10 mm (when more than 40 μL oil in the discs). P. fluorescens was not inhibited by the tested essential oils.

  8. Antibacterial activity of essential oils, their blends and mixtures of their main constituents against some strains supporting livestock mastitis.

    Science.gov (United States)

    Fratini, Filippo; Casella, Sergio; Leonardi, Michele; Pisseri, Francesca; Ebani, Valentina Virginia; Pistelli, Laura; Pistelli, Luisa

    2014-07-01

    Ten of the most known and used commercial essential oils (Cinnamomum zeylanicum L., Citrus bergamia Risso, Eucalyptus globulus Labill., Foeniculum vulgare Mill., Origanum majorana L., Origanum vulgare L., Rosmarinus officinalis L., Satureja montana L., Thymus vulgaris L. ct. carvacrol, Thymus vulgaris L. ct. thymol) were tested against six bacteria strains Staphylococcus aureus, Staphylococcus chromogenes, Staphylococcus sciuri, Staphylococcus warneri, Staphylococcus xylosus and Escherichia coli, responsible for mastitis in animals. The best results were achieved by S. montana, T. vulgaris ct. thymol and O. vulgare. Two binary mixtures of essential oils (EOs) were prepared of S. montana and T. vulgaris ct. thymol (ST) and of S. montana and O. vulgare (SO). The ST mixture exhibited the best inhibitory activity against all the tested bacterial strains. Two artificial mixtures of carvacrol/thymol (AB) and carvacrol/thymol/p-cymene (CD) were prepared and tested against all of the bacterial strains used. The results exhibited a general reduction of the inhibitory activity of mixture AB, although not reaching the inhibition of the ST and SO mixtures. However the mixture CD presented an apparent strong inhibition against S. aureus and S. sciuri. The EO mixtures and the mixture CD represent promising phytotherapic approaches against bacteria strains responsible for environmental mastitis.

  9. Review of Scientific Evidence of Medicinal Convoy Plants in Traditional Persian Medicine.

    Science.gov (United States)

    Sadati, Seyede Nargess; Ardekani, Mohammad Reza Shams; Ebadi, Nastaran; Yakhchali, Maryam; Dana, Azadeh Raees; Masoomi, Fatemeh; Khanavi, Mahnaz; Ramezany, Farid

    2016-01-01

    One concept used in traditional Persian medicine (TPM) for multidrug therapy is that of the convoy drug (Mobadregh). According to TPM texts, convoy drugs are substances (or drugs), which facilitate the access of drugs or foods to the whole body or to specific organs. This study reviewed some convoy drugs presented in TPM, their biological effects, and their probable interactions with main drugs, considering the increased absorption through inhibition of P-glycoprotein (P-gp) efflux function, bioavailability-enhancing effects, and decreased metabolism of the main drug using electronic databases including PubMed, Scopus, ScienceDirect, and Google Scholar in November and December, 2013. Recent studies have proven the beneficial effects of Crocus sativus L. (saffron) and camphor on the heart and brain, the cerebral therapeutic effects of Asarum europaeum (hazelwort), the hepatoprotective effects of Cichorium intybus (chicory), and Apium graveolens (celery) seeds, and the diuretic effects of Cinnamomum zeylanicum (cinnamon), and Cucumis melo (melon) seeds. The effects of vinegar in targeting the liver and brain have also been demonstrated. An evaluation of the results demonstrated that the suggested convoy drugs, including Piper nigrum (black pepper), Piper longum (long pepper), red wine, Camellia sinensis (tea), hazelwort, Mentha longifolia (pennyroyal), Anethum graveolens (dill), Foeniculum vulgare (fennel), cinnamon, and Sassafras albidum (sassafras) can increase the bioavailability of coadministered drugs by inhibition of P-gp or cytochrome P450s (CYP450s) or both of them. This evidence could be a good basis for the use of these agents as convoys in TPM.

  10. Spasmolytic effect of traditional herbal formulation on guinea pig ileum

    Science.gov (United States)

    Kumar, Dushyant; Ganguly, Kuntal; Hegde, H. V.; Patil, P. A.; Kholkute, S. D.

    2015-01-01

    Background: The herbal formulation consisting of Andrographis paniculata Nees., Cassia fistula L., Foeniculum vulgare Mill. and Cuminum cyminum L. is widely used by the local traditional practitioners in rural Northern Karnataka for spasmodic abdominal pain. Objective: The present study was undertaken to evaluate safety and spasmolytic effect of poly-herbal formulation. Materials and Methods: Acute toxicity studies were carried out in Swiss mice, as per the Organization for Economic Co-operation and Development (OECD) guidelines. The spasmolytic activity of the formulation was studied in isolated guinea pig ileum model using histamine and acetylcholine as agonists. The data were analyzed by one-way ANOVA, followed by Dunnetts post-hoc test and P ≤ 0.05 was considered as significant. Results: The formulation did not show any adverse toxic effects and found to be safe. It also showed significant (P < 0.05) relaxation in different agonist like histamine and acetylcholine-induced contractions in guinea pig ileum. Conclusion: Antispasmodic activity of the herbal formulation can be attributed to its atropine-like activity. The present findings, therefore, support its utility in spasmodic abdominal pain. PMID:26604555

  11. Medicinal plants used for ophthalmological problems in Navarra (Spain).

    Science.gov (United States)

    Calvo, M Isabel; Cavero, Rita Yolanda

    2016-08-22

    Several plants have been found to have effective against number of ophthalmological problems in Navarra. Information was collected using semi-structured ethnobotanical interviews with 686 informants in 267 locations. In order to confirm the pharmacological application of the uses more cited by the informants, a literature review was conducted. A total of 57 pharmaceutical uses were reported, for 19 plants and 13 families, mainly represented by Asteraceae. The most frequently used parts were inflorescences, flowers, aerial parts, leaves and flowered aerial parts. The related affections fell into eleven categories: bloodshot eyes, watery eyes and wounds, improve vision, irrited eyelids, rheums and styes, tired eyes, conjunctivitis, eyewash, ocular problems in general. The most cited plants were: Chamaemelum nobile (L.) All., Santolina chamaecyparissus L. ssp. squarrosa (DC.) Nyman, Sambucus nigra L. ssp. nigra, Rosa agrestis Savi and Calendula officinalis L. None of them have been pharmacologically validated by Official International Organisms. From the therapeutic point of view, Allium sativum L., Foeniculum vulgare Mill., C. officinalis, and S. chamaecyparissus ssp. squarrosa deserve special attention, because ethnobotanical and pharmacological studies suggest that these medicinal plants are effective for ophthalmological problems. The present study constitutes a good basis for further phytochemical and pharmacological research of these four plants, which could be of interest in the design of new inexpensive, effective and safe drugs. The remaining plants are needed to be screened through standard pharmacological and clinical procedures for their activities. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  12. Interspecific Associations between Cycloneda sanguinea and Two Aphid Species (Aphis gossypii and Hyadaphis foeniculi) in Sole-Crop and Fennel-Cotton Intercropping Systems.

    Science.gov (United States)

    Fernandes, Francisco S; Ramalho, Francisco S; Malaquias, José B; Godoy, Wesley A C; Santos, Bárbara Davis B

    2015-01-01

    Aphids cause significant damage to crop plants. Studies regarding predator-prey relationships in fennel (Foeniculum vulgare Mill.) and cotton (Gossypium hirsutum L.) crops are important for understanding essential ecological interactions in the context of intercropping and for establishing pest management programs for aphids. This study evaluated the association among Hyadaphis foeniculi (Passerini) (Hemiptera: Aphididae), Aphis gossypii Glover (Hemiptera: Aphididae) and Cycloneda sanguinea (L.) (Coleoptera: Coccinellidae) in cotton with coloured fibres, fennel and cotton intercropped with fennel. Association analysis was used to investigate whether the presence or absence of prey and predator species can indicate possible interactions between aphids and ladybugs. Significant associations among both apterous and alate H. foeniculi and C. sanguinea were observed in both the fennel and fennel-cotton intercropping systems. The similarity analysis showed that the presence of aphids and ladybugs in the same system is significantly dependent on the type of crop. A substantial amount of evidence indicates that the presence of the ladybug C. sanguinea, is associated with apterous or alate A. gossypii and H. foeniculi in fennel-cotton intercropping system. We recommend that future research vising integrated aphid management taking into account these associations for take decisions.

  13. Larvicidal Activity of Essential Oils of Apiaceae Plants Against Malaria Vector, Anopheles Stephensi

    Directory of Open Access Journals (Sweden)

    Y Salim Abadi

    2011-12-01

    Full Text Available Background: Plant extracts and oils may act as alternatives to conventional pesticides for malaria vector control. The aim of this study was to evaluate the larvicidal activity of essential oils of three plants of Apiaceae family against Anophe­les stephensi, the main malaria vector in Iran. Methods: Essential oils from Heracleum persicum, Foeniculum vulgare and Coriandrum sativum seeds were hydro distil­lated, then their larvicidal activity were evaluated against laboratory-reared larvae of An. stephensi according to stan­dard method of WHO. After susceptibility test, results were analysis using Probit program.Results: Essential oils were separated from H. persicum, F. vulgare and C. sativum plants and their larvicidal activi­ties were tested. Result of this study showed that F. vulgare oil was the most effective against An. stephensi with LC50 and LC90 values of 20.10 and 44.51 ppm, respectively.Conclusion: All three plants essential oil can serve as a natural larvicide against An. stephensi. F. vulgare oil exhib­ited more larvicidal properties.

  14. Repellency of aerosol and cream products containing fennel oil to mosquitoes under laboratory and field conditions.

    Science.gov (United States)

    Kim, Soon-Il; Chang, Kyu-Sik; Yang, Young-Cheol; Kim, Byung-Seok; Ahn, Young-Joon

    2004-11-01

    The repellency of fennel (Foeniculum vulgare Miller)-containing products (5% aerosol and 8% cream) against mosquitoes was compared with those of citronella oil, geranium oil and deet, as well as three commercial repellents, Baby Keeper cream containing IR3535, MeiMei cream containing citronella and geranium oils, and Repellan S aerosol containing 19% N,N-diethyl-m-toluamide (deet) under laboratory and field conditions. In a laboratory study with female Aedes aegypti (L), fennel oil exhibited good repellency in a release-in-cage test and repellency in skin and patch tests of the oil was comparable with those of citronella and geranium oils. In paddy field tests with five human volunteers, 5% and 8% fennel oil-containing aerosol and cream produced 84% and 70% repellency, respectively, at 90 min after exposure, whereas Baby Keeper cream and MeiMei cream gave 71% and 57% repellency at 90 min after exposure, respectively, and Repellan S aerosol gave 89% repellency at 210 min. The species and ratio of mosquitoes collected were the genera Culex (44.1%), Anopheles (42.2%), Aedes (7.8%) and Armigeres (5.9%). Fennel oil-containing products could be useful for protection from humans and domestic animals from vector-borne diseases and nuisance caused by mosquitoes.

  15. Assessment of fennel aphids (Hemiptera: Aphididae) and their predators in fennel intercropped with cotton with colored fibers.

    Science.gov (United States)

    Ramalho, F S; Fernandes, F S; Nascimento, A R B; Nascimento Júnior, J L; Malaquias, J B; Silva, C A D

    2012-02-01

    The fennel aphid, Hyadaphis foeniculi (Passerini) (Hemiptera: Aphididae) is a major pest of fennel, Foeniculum vulgare Miller in northeast region of Brazil. We hypothesize that intercropping can be used as an alternative pest management strategy to reduce aphid yield loss in fennel. Thus, we investigated the severity of fennel plant damage in relation to infestation by the fennel aphid and predation by Cycloneda sanguinea (L.) (Coleoptera: Coccinellidae) (spotless lady beetle), green lacewing, Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae), and Scymnus spp. (Coleoptera: Coccinellidae) in sole fennel plots and plots of fennel intercropped with cotton with colored fibers. The fennel aphid populations in nontreated plots were significantly larger in sole fennel plots than in intercropped plots. The highest densities of C. sanguinea, green lacewings and Scymnus spp., associated with the suppression of fennel aphid populations was found in fennel in the intercropping systems. Fennel aphids reduced the fennel seed yield by 80% in the sole fennel plots compared with approximately 30% for all intercropping systems. The results obtained in this research are of practical significance for designing appropriate strategies for fennel aphid control in fennel-cotton intercropping systems. In summary, intercropping fennel with cotton with colored fibers apparently promoted biocontrol of fennel aphid in fennel.

  16. Radical scavenging and iron-chelating activities of some greens used as traditional dishes in Mediterranean diet.

    Science.gov (United States)

    El, Sedef Nehir; Karakaya, Sibel

    2004-02-01

    This study aimed at evaluating the antioxidative activity of nine different families of greens. Raphanus raphanistrum (wild radish), Anchusa azurea (bugloss), Daucus carota (wild carrot), Sonchus oleraceus (sowthistle), Papaver rhoeas (corn poppy), Malva sylvestris (blue mallow), Foeniculum vulgare (fennel), Cichorium intybus (chicory) and Salicornia europaea (jointed glasswort) are native to the Mediterranean and are commonly consumed as a salad or an ingredient in some recipes. The antioxidative activities, including the radical scavenging effects, inhibition of hydrogen peroxide (H(2)O(2)), and Fe(2+)-chelating activity, were studied. All samples showed antioxidant activity as a radical scavenger in the experiment using the DPPH* radical. The ratio between the slopes of the kinetic model was used to compare antioxidant efficiency of different greens. Greens also possessed antioxidative activity toward H(2)O(2). Especially, greens exhibited a marked scavenging effect on H(2)O(2) at 0.2 g/ml concentration. The Fe(2+) ion-chelating activities of the samples except jointed glasswort were greater than 70%. The antioxidant activity of samples with different methods based on the inhibition of different reactions could not be compared. The current dietary guidelines include recommendations for an increase in the consumption of plant foods. Greens should provide an optimal supply of antioxidant substances in the diet.

  17. Study on Growth Behavior of Fennel from Different Areas%不同地区茴香生长习性的比较研究

    Institute of Scientific and Technical Information of China (English)

    王晓敏; 俞婷; 李军; 高艳明; 李建设

    2015-01-01

    研究以产自宁夏海原、内蒙古托县、甘肃玉门、德国共4个地区的茴香(Foeniculum Vulgare)为材料在宁夏银川栽培,通过定期测定植株株高、最大叶长、最大叶宽、最大叶的小叶对数、全株干重,以及小花数、主花序径、小花序径、小花径等指标,对其生长习性进行了比较研究.结果表明,内蒙古托县和德国地区茴香的株高、全株鲜、干重等指标显著高于其他2个地区;开花最早的是宁夏海原地区的茴香,其次是甘肃玉门和内蒙古托县,德国茴香在调查期近8个月内并未开花.

  18. Studies on the Spreading Distance of Pollen and Rule of Isolation Pollination in Fennel%茴香花粉传播距离及隔离结实规律

    Institute of Scientific and Technical Information of China (English)

    王明友; 高方胜; 张红

    2013-01-01

    运用花粉捕捉法和15N示踪技术研究了茴香(Foeniculum vulgare Mill.)不同开花时期在不同栽培环境下的花粉风力和昆虫传播距离,同时研究了花期隔离对茴香种子产量的影响.结果表明,茴香生长环境是影响花粉风力传播距离和数量的重要因素,在空旷的蔬菜地最远传播距离为900 m,棉花区亦为900m,玉米区花粉传播距离最远为450m.通过对昆虫的传播距离研究可知,茴香良种繁育有效距离在蔬菜区(空旷地)应在2200m外,中秆作物区(棉花区)应在2000m外,高秆隔离区(玉米区)应在1 200m外.与全隔离区相比,对照(自然授粉)和上部隔离授粉分别增产251.4%和125.0%.

  19. TLC and GC-MS Analyses of Essential Oil Isolated from Macedonian Foeniculi fructus

    Directory of Open Access Journals (Sweden)

    Menče Najdoska

    2011-05-01

    Full Text Available The essential oil of Foeniculi fructus, the ripe fruit from the plant Foeniculum vulgare Mill. ssp. vulgare var. dulce Batt. and Trab. (sweet fennel, македонски анасон was isolated and the main components were identified and quantified using thin-layer chromatography (TLC and gas chromatography and mass spectrometry (GC-MS. The Aetherloeum Foeniculi had pale yellow color and characteristic pleasant odor and was obtained in 3.2% yield (by weight from the plant fruits. The main components of the oil were: trans-anethole, 1, (70%, p-anisaldehyde, 2, (19% estragole, 3, (6% and anisacetone, 4, (2%. The identity of the components was determined by matching the mass spectra to the library spectra. The identity of the main component, trans-anethole, was confirmed directly by isolation using column chromatography and indirectly by potassium permanganate oxidation of the essential oil. The oxidation product from this reaction, pmethoxybenzoic acid, 16, was isolated and characterized. The potential products of autooxidation of trans-anethole (epoxy anethole, anethole glycol and 16 were not detected in the freshly obtained essential oils.

  20. 间种芳香植物对蔬菜生长及虫害的影响

    Institute of Scientific and Technical Information of China (English)

    叶珺琳; 郭国保; 潘春香; 郭克婷

    2014-01-01

    以迷迭香(Rosmarinus officinatis)、茴香(Foeniculum vulgare)、薄荷(Mentha haplocalyx)等3种芳香草本植物为材料,采取与蕹菜间作种植方式,探讨3种芳香植物中特殊的挥发性成分对蕹菜生长及害虫的驱避作用。结果表明:茴香、迷迭香、薄荷与蕹菜间作后,对蕹菜的株高、株幅、叶片数、分株、产量均有一定的影响。由于迷迭香、薄荷根系发达,对蕹菜的生长产生影响,最终导致蕹菜产量较低。但芳香植物驱避虫害效果显著,与芳香植物间种的蕹菜虫害明显减少,且随着蕹菜的生长,芳香植物的防虫效果更加显著。其中,迷迭香防虫效果最佳且稳定,茴香次之,薄荷的效果不稳定。

  1. Allelopathic Effects of Johnsongrass on Germination and Early Seedling Growth of Basil, Black Cumin, Cummin, Fennel, Isabgol and Psyllium

    Directory of Open Access Journals (Sweden)

    M Asgharipoor

    2012-12-01

    Full Text Available The allelopathic effect of weeds frequently inhibited the germination and seedling growth of crops. Johnsongrass (Sorghum halepens is known to contain water-soluble substances that are allelopathic. To identify the allelopathic effect of Johnsongrass, the effect of different aqueous extracts (0, 5, 10, 20, 30, 40 and 50 g L-1 obtained from roots and leaves of Johnsongrass on the seed germination and early seedling growth of some popular medicinal plants of Iran [basil (Ocimum basilicum, black cumin (Nigella sativa, cumin (Cuminum cyminum, fennel (Foeniculum vulgare, isabgol (Plantago ovate and psyllium (Plantago psyllium] were studied by bioassay. Both the root and leaf extracts of Johnsongrass in most cases inhibited the seed germination and early seedling growth of isabgol, psyllium, fennel and basil, while the seed germination and the early seedling growth of black cumin and cummin was simulated by both extracts at lower concentrations. However, this advantage was not found in higher concentrations, at which the extracts mostly had a negative effect on Nigella sativa and cumin. Leaf extracts was more effective to germination and early seedling growth than the root extract. The effectiveness of these extracts on the root growth was greater than that of the shoot growth of the test plants. The degree of sensitivity can be classified averaged across all extract concentrations in order of decreasing inhibition as follow: psyllium, isabgol, fennel, basil, black cumin and Cummin.

  2. Report of some Fungi From of the Condiments of Daily Use in Storage

    Directory of Open Access Journals (Sweden)

    C. B. Lal

    1975-10-01

    Full Text Available The isolation of fungi associated with the condiments of daily storage has been done. Fourteen species of fungi viz; Circinella mucoroides, C. muscae, Aspergillus candidus, A. nidulans, A. niger, A. niveus, Aspergillus species, Penicillium Frequentans, Alternaria humicola, Curvalaria tetramera, Monilia geophila, Fusarium species and Rhizoctonia species have been isolated from five important condiments Coriandrum sativum L.(Dhania, Cuminum eyminum L(Jeera, Curcuma longa L(Haldi, Foeniculum vulgare Miller (Saunf and Trigonella foenum graecum L.(Menthi in storage have been isolated Penicillium frequentans and Curvularia tetramera could be isolated from only one source Coriandrum sativum and Trigonella foenumgraecum respectively. Circinella mucoroides and C. muscae are the first record from these sources in our country. Monilia geophila and Rhizoctonia species too have been reported for the first time from curcuma longa and Trigonella foenumgraecum respectively. It may be added that the fungi found in association with the condiments may be pathogenic to the plants but if the condiments are thoroughly dried, exposed to bright sunlight and and cleaned before utilization, they can be rendered safe for human consumption.

  3. DNA条形码技术鉴定一种植物样品的研究%Study on Identification of an Unknown Sample with DNA Barcoding

    Institute of Scientific and Technical Information of China (English)

    许亮; 刘春生; 杨燕云; 王冰; 康廷国

    2014-01-01

    目的:对一种未知的破碎的植物样品进行品种鉴定.方法:综合采用性状鉴定、显微鉴定、DNA条形码鉴定等方法,DNA条形码鉴定经过DNA提取、PCR扩增、测序,对ITS序列数据经Blast比对法、特异位点法、系统发育法三种方法进行分析.结果:该未知样品为伞形科植物茴香Foeniculum vulgare Mill.去掉果皮的种子.结论:中药鉴定技术能够解决实际工作中的未知样品品种鉴定问题,DNA条形码技术为中药鉴定提供一种新的方法,在未知样品的鉴定中发挥重要的参考作用.

  4. EFFECT OF FENNEL WATER EXTRACTS ON REDUCTION OF FEEDING OF PEA LEAF WEEVIL

    Directory of Open Access Journals (Sweden)

    Barbara Biniaś

    2016-11-01

    Full Text Available The objective of the study was to examine the effect of aqueous extracts from fennel (Foeniculum vulgare Mill. seeds at 2%, 5%, 10% and 20% concentrations on the feeding of peal leaf weevil (Sitona lineatus L. on broad bean (Vicia faba L.. The experiment was conducted in the laboratory, in six replicates. Feeding intensity assessment was conducted by dipping leaves of broad bean in respective solutions of the extracts and determining the area of broad bean leaves, eaten by pea leaf weevil beetle in the 12 hour intervals. In addition, absolute deterrence index and palatability index were calculated. As a result of the observation no significant limiting effect of fennel seed aqueous extracts on the feeding of the pea leaf weevil females was shown. All of the used fennel extracts had inhibitory effect on the feeding of male S. linetaus and the strongest effect of extracts was observed in the first 36 hours of the experiment. The high values of the palatability index (particularly for the females with relatively low absolute deterrence index, indicate limited possibilities of the use of aqueous extracts from fennel seeds for the protection against the feeding of the beetles from the genus Sitona.

  5. New oilseed crops on the horizon

    Energy Technology Data Exchange (ETDEWEB)

    Princen, L.H.

    Fats and oils for food uses are now plentiful on a worldwide basis. Tallow, lard and fish oils, as well as vegetable oils, such as those derived from soybean, sunflower, palm, rapeseed, peanut and cottonseed, are often overproduced. Although many of these products are also used for industrial chemicals, they often are not of the most favorable composition for nonfood applications. A search for new oilseed crops with more advantageous oil composition has led to the development of excellent candidates that are now close to commercial acceptance. Among them are Crambe, Limnanthes, Vernonia, Sapium and Simmondsia. Other crops are at a much lower stage of development but also have excellent potential. They include Cuphea, Foeniculum, Stokesia, Lesquerella and Lunaria. One new oilseed crop which is being considered for hydrocarbon-like fuel is the Chinese tallow tree (Sapium Sebiferum). Recent research estimates predict that seed yield could amount to 10,000 lbs. per acre. At 40% seed lipid levels, this can translate to 4000 lbs. per acre of fuel, more than any other plant species now growing in the US. 24 references, 6 figures, 5 tables.

  6. Exergetic analysis and evaluation of a new application of gas engine heat pumps (GEHPs) for food drying processes

    Energy Technology Data Exchange (ETDEWEB)

    Gungor, Aysegul [Department of Mechanical Engineering, Faculty of Engineering, Gediz University, Izmir (Turkey); Erbay, Zafer [Department of Food Engineering, Faculty of Engineering, Ege University, 35100 Izmir (Turkey); Hepbasli, Arif [Department of Mechanical Engineering, Faculty of Engineering, Ege University, 35100 Izmir (Turkey)

    2011-03-15

    In this study, three medicinal and aromatic plants (Foeniculum vulgare, Malva sylvestris L. and Thymus vulgaris) were dried in a pilot scale gas engine driven heat pump drier, which was designed, constructed and installed in Ege University, Izmir, Turkey. Drying experiments were performed at an air temperature of 45 C with an air velocity of 1 m/s. In this work, the performance of the drier along with its main components is evaluated using exergy analysis method. The most important component for improving the system efficiency is found to be the gas engine, followed by the exhaust air heat exchanger for the drying system. An exergy loss and flow diagram (the so-called Grassmann diagram) of the whole drying system is also presented to give quantitative information regarding the proportion of the exergy input dissipated in the various system components, while the sustainability index values for the system components are calculated to indicate how sustainability is affected by changing the exergy efficiency of a process. Gas engine, expansion valve and drying ducts account for more than 60% amount of exergy in the system. The exergetic efficiency values are in the range of 77.68-79.21% for the heat pump unit, 39.26-43.24% for the gas engine driven heat pump unit, 81.29-81.56% for the drying chamber and 48.24-51.28% for the overall drying system. (author)

  7. Antimycotic efficacy of Iranian medicinal plants towards dermatophytes obtained from patients with dermatophytosis

    Institute of Scientific and Technical Information of China (English)

    Ali Reza Khosravi; Hojjatollah Shokri; Zohreh Farahnejat; Reza Chalangari; Martis Katalin

    2013-01-01

    AIM:Nine plant essential oils (EOs),including those from Artemisia sieberi,Cuminum cyminum,Foeniculum vulgare,Heracleum persicum,Menta spicata,Nigella sativa,Rosmarinus officinalis,Zataria multiflora and Ziziphora clinopodioides,were evaluated for their anti-dermatophytic properties.METHODS:The tested dermatophytes included Trichophyton mentagrophytes (No.32),T.rubrum (No.29),Epidermophytonfloccosum (No.19),Microsporum gypseum (No.11) and M.canis (No.42).The susceptibility tests of plant oils in terms of minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) were performed by the broth microdilution technique as described by the Clinical Laboratory Standard Institute.RESULTS:All EOs studied were active against the dermatophytes.The MICs recorded for the plant oils tested ranged from 0.25 to 4 mg·mL-1.The most significant activity was observed with A.sieberi,showing a lower MIC against dermatophytes than other plant oils (P < 0.05).CONCLUSION:Data from this study indicated that several Iranian medicinal plant oils,mainly A.sieberi,are active in vitro against different dermatophyte species,suggesting their potential use for the topical treatment of dermatophytoses.

  8. 球茎茴香的贮藏技术

    Institute of Scientific and Technical Information of China (English)

    高丽朴; 郑淑芳

    2006-01-01

    球茎茴香(Foeniculum vulgare Mill.var.dulce Batt.et Trab.),又称结球茴香、甜茴香,为伞形花科茴香属茴香种的一个变种,主要食用部位为叶鞘基部相互抱合形成的球状茎,具有独特芳香和甜味,较食叶茴香风味更为浓郁。在元旦、春节前适当做些贮藏可有效提高经济效益。球茎茴香贮藏的主要问题是失水萎蔫、叶鞘变糠和褐变。防止这些问题的主要方法是控制好适宜温度和选择适宜的包装。

  9. 球茎茴香及其栽培

    Institute of Scientific and Technical Information of China (English)

    程春林

    2001-01-01

    @@ 球茎茴香又称结球茴香,为伞形科茴香属的一个变种(Foeniculum vulgare Mill.),2年生草本植物.球茎茴香以其柔嫩的鳞茎及嫩叶供食用.它营养丰富,含有大量的β-胡萝卜素、Vc和各种人体必需的氨基酸,是一种营养价值较高的新型蔬菜.嫩叶微香,可做馅;球茎可切成薄片,炒、煮、做汤或凉拌、腌渍、生食,均具独特风味;榨汁可作西菜的重要调料,是高级宾馆的上乘菜肴.近年来,球茎茴香开始在我国广东、云南、贵州、上海、浙江、湖北和江苏等省市种植,具有较高的经济效益.

  10. Research Progress of Antibacterial Effect of Several Kinds of Spices%几种香辛料抗菌作用的研究进展

    Institute of Scientific and Technical Information of China (English)

    黄敏欣; 赵文红; 白卫东; 钱敏

    2015-01-01

    Spices have unique irritating aroma,being used for the seasoning of food to provide flavor and aroma.Importantly,spices have certain antibacterial effects,which are effective sources of natural preservative. In this paper, the antibacterial effects of several kinds of spices, such as Foeniculum vulgare Mill,onion,acorus calamus,licorice are summarized.It is expected to make some contribution to the development of natural preservatives.%香辛料具有一种独特的刺激性香味,用于食品的调味,赋予香气,同时还具有一定的抗菌作用,成为天然防腐剂的重要来源之一。综述小茴香、洋葱、菖蒲、甘草等几种香辛料的抗菌作用,以期为小茴香、洋葱、菖蒲、甘草等几种香辛料作为天然防腐剂的开发有所贡献。

  11. 茴香高效种植模式及配套技术

    Institute of Scientific and Technical Information of China (English)

    肖占文

    2004-01-01

    茴香(Foeniculum Vulgare Mill)属伞形花科,茴香属,多年生草本植物,有小茴香、大茴香和球茎茴香等不同类型,因其具有较高的营养价值和药用价值,在我国广泛栽培。甘肃省河西走廊栽培茴香历史悠久,得天独厚的自然条件有利于茴香高产优质栽培。长期以来,以旱薄地单种为主,效益低下。近几年,随着种植制度改革和栽培水平的提高,规模逐年增大,截止2002年,走廊内茴香面积达1.6万hm,成为当地优化种植结构,增加农民收入,促进经济发展的新型产业,为了进一步提高种植水平和效益,通过作物种群间的合理搭配,科学田间配置,探索出了几种高效立体种植模式。

  12. 天然调味香料小茴香挥发油的研究进展%Research Progress on EssentiaI OiI from NaturaI Spice Foeniculumvulgare MiII

    Institute of Scientific and Technical Information of China (English)

    张禄捷; 刘韬; 李荣; 姜子涛

    2015-01-01

    The essential oil from natural spice Foeniculum vulgare Mill has antibacterial, anti-inflammatory,analgesic and other bioactivities and it has great application prospects in the fields of spices and medicine.In recent years,it has aroused the research enthusiasm of food scientists, chemists,pharmacologists and biologists.In this paper,the recent studies on the existed research results from the aspects of chemical components,extraction methods,bioactivities and application prospects of essential oils from F. vulgare Mill are reviewed.%天然调味香料小茴香挥发油具有抗菌、消炎、止痛等生物活性,在调味品和医药等领域有着很大的应用前景,近年来激起了食品、化学、医药和生物学家的研究热情。从小茴香挥发油的化学成分、提取方法、生物活性及应用前景出发,对已有的研究成果进行了综述。

  13. Repellency of Aphidius gifuensis and Pachyneuron aphidis to various odour sources%烟蚜茧蜂和蚜虫宽缘金小蜂对不同味源的趋避性

    Institute of Scientific and Technical Information of China (English)

    张英; 杨硕媛; 赵进龙; 谷星慧; 任伟; 高艳飞; 吴伟

    2012-01-01

    用“Y”型嗅觉仪观测了烟蚜茧蜂和蚜虫宽缘金小蜂对几种非寄主植物材料的趋避性.结果表明,烟蚜茧蜂对茼蒿叶的选择呈显著负趋性;蚜虫宽缘金小蜂对花椒叶、茴香叶的选择表现出明显的负趋性,但葱叶对其具有引诱作用;烟蚜茧蜂和蚜虫宽缘金小蜂混合组对薄荷叶、鱼腥草叶、紫金泽兰叶、番茄叶和姜叶的选择也表现出明显的负趋性.%Repellency of Aphidius gifuensis and Pachyneuron aphidis to several kinds of non-host plant materials was investigated with "Y" olfactometer. Results showed that Aphidius gifuensis had significant negative tropism to Chrysanthemum leaves. Pachyneuron aphidis had negative tropism to Zanthoxylum bungeanum leaves and Foeniculum vulgare leaves , but was attracted to Allium fistulosum leaves. The mixed group of Aphidius gifuensis and Pachyneuron aphidis showed negative tropism to Mentha haplocalyx leaves, Houttuynia cordata leaves, Eupatorium adenophorum leaves, Lycopersicon leaves and Ginger leaves.

  14. Seed germination in response to chemicals: effect of nitrogen and pH in the media.

    Science.gov (United States)

    Pérez-Fernández, M A; Calvo-Magro, E; Montanero-Fernández, J; Oyola-Velasco, J A

    2006-01-01

    Seed germination generally presents a peak in the next growing season after a fire. Among other factors associated with fire are the increase of soil nitrogen and changes in the pH of the soil. In this study, we addressed the question, whether or not the germination response of eight species is linked with the increase in pH and nitrogenous compounds in the germination media? We assessed the separate and combined effects of nitrogenous compounds and pH on the percentage and rate of germination of seeds of Medicago arabica (L.) Hudson, Epilobium hirsutum L., Foeniculum vulgare Miller, Daucus carota L., Thapsia villosa L., Cynosurus cristatus L., Dactylis glomerata L. and Rumex crispus L. All these species are well represented in the Mediterranean ecosystems of the central-west Spain. Water and CaCl2 were used as controls. Nitrogenous compounds increased percent germination (level) and rate in three of the species studied. High pH negatively affected the germination rate of seeds from most species, but had no effect on the per cent germination of any of the species. The higher concentration of the nutritious solutions affected negatively the germination level and rate. The different germination responses of seeds of the studied species could not be exclusively attributed to pH values in the media, whereas the amount and form of Nitrogen in the media has a greater effect on it. These differences in germination are species dependent.

  15. Fungal Control of Pathogenic Fungi Isolated From Some Wild Plants in Taif Governorate, Saudi Arabia

    Directory of Open Access Journals (Sweden)

    Abou-Zeid, A. M.

    2008-01-01

    Full Text Available Twenty two plants were collected from Taif Governorate and identified as: Aerva lanata, Arnebia hispidissima, Artemisia judaica, Artemisia monosperma, Asphodelus aestives, Avena barbata, Capparis dcidua, Eucalyptus globulus, Euphorbia glomerifera, Foeniculum vulgare, Forsskaolea tenacissima, Juniperus procera, Launaea mucronata, Launaea sonchoides, Medicago sativa, Opuntia ficus, Phagnalon sinaicum, Prunus persica, Pulicaria crispa, Punica granatum, Rumex dentatus and Trichodesma calathiforme. Pathogenic fungi were isolated from some of these plants and identified as Alternaria alternata, Cephalosporium madurae, Cladosporium herbarum, Fusarium oxysporum, Humicola grisea, Penicillium chrysogenum and Ulocladium botrytis. Four antagonistic isolates were tested, 2 from Gliocladium fungus and 2 from Trichoderma fungus. We found that all the four antagonistic isolates (G. deliquescens, G. virens, T. viride and T. hamatum significantly inhibited the radial growth of the pathogenic fungi tested, with different ratios. The results indicated that the antibiotics produced by the antagonists were more effective than the fungus itself and differ with different fungi. Coating plant stems with antagonists or with antagonist extracts reduce the severity of the disease but not prevent it in all tested pathogens.

  16. Aktivitas minyak dan serbuk enam spesies tumbuhan terhadap peneluran dan Mortalitas Callosobruchus sp. (Coleoptera: Bruchidae

    Directory of Open Access Journals (Sweden)

    Dadang Dadang

    2017-02-01

    Full Text Available Storage pests remain one of the main problems in storage. The pest attacks occur not only in the field, but also in storage. Control should be done to maintain the quality of storedproducts. The environmentally friendly measures should be implemented to avoid negative impacts to the environment and human being. The objective of this research was to study the mortality and oviposition deterrence caused by powder and oil of six plant species against Callosobruchus sp. (Coleoptera: Bruchidae. Flowers of Eugenia aromatica, roots of Vetiveria zizanioides, leaves of Pogostemon cablin, seeds of Ricinus communis, seeds of Foeniculum vulgare, and stems of Cymbopogon citratus were air-dried and milled to yield powder. Oil of six plant species were purchased from local market. Oviposition deterrent bioassay was conducted by no-choice method for both powder and oil, while mortality bioassay was conducted by topical application and residual methods for plant oil only. Several plant species showed high biological activity to Callosobruchus sp. Powder of E. aromatica and oil of V. zizanioides caused high oviposition deterrence, while oil of V. zizanioides and E. aromatica caused high mortality.

  17. Interspecific Associations between Cycloneda sanguinea and Two Aphid Species (Aphis gossypii and Hyadaphis foeniculi in Sole-Crop and Fennel-Cotton Intercropping Systems.

    Directory of Open Access Journals (Sweden)

    Francisco S Fernandes

    Full Text Available Aphids cause significant damage to crop plants. Studies regarding predator-prey relationships in fennel (Foeniculum vulgare Mill. and cotton (Gossypium hirsutum L. crops are important for understanding essential ecological interactions in the context of intercropping and for establishing pest management programs for aphids. This study evaluated the association among Hyadaphis foeniculi (Passerini (Hemiptera: Aphididae, Aphis gossypii Glover (Hemiptera: Aphididae and Cycloneda sanguinea (L. (Coleoptera: Coccinellidae in cotton with coloured fibres, fennel and cotton intercropped with fennel. Association analysis was used to investigate whether the presence or absence of prey and predator species can indicate possible interactions between aphids and ladybugs. Significant associations among both apterous and alate H. foeniculi and C. sanguinea were observed in both the fennel and fennel-cotton intercropping systems. The similarity analysis showed that the presence of aphids and ladybugs in the same system is significantly dependent on the type of crop. A substantial amount of evidence indicates that the presence of the ladybug C. sanguinea, is associated with apterous or alate A. gossypii and H. foeniculi in fennel-cotton intercropping system. We recommend that future research vising integrated aphid management taking into account these associations for take decisions.

  18. Spasmolytic effect of traditional herbal formulation on guinea pig ileum

    Directory of Open Access Journals (Sweden)

    Dushyant Kumar

    2015-01-01

    Full Text Available Background: The herbal formulation consisting of Andrographis paniculata Nees., Cassia fistula L., Foeniculum vulgare Mill. and Cuminum cyminum L. is widely used by the local traditional practitioners in rural Northern Karnataka for spasmodic abdominal pain. Objective: The present study was undertaken to evaluate safety and spasmolytic effect of poly-herbal formulation. Materials and Methods: Acute toxicity studies were carried out in Swiss mice, as per the Organization for Economic Co-operation and Development (OECD guidelines. The spasmolytic activity of the formulation was studied in isolated guinea pig ileum model using histamine and acetylcholine as agonists. The data were analyzed by one-way ANOVA, followed by Dunnetts post-hoc test and P ≤ 0.05 was considered as significant. Results: The formulation did not show any adverse toxic effects and found to be safe. It also showed significant (P < 0.05 relaxation in different agonist like histamine and acetylcholine-induced contractions in guinea pig ileum. Conclusions: Antispasmodic activity of the herbal formulation can be attributed to its atropine-like activity. The present findings, therefore, support its utility in spasmodic abdominal pain.

  19. Effects of oral dosage form and storage period on the antioxidant properties of four species used in traditional herbal medicine.

    Science.gov (United States)

    Guimarães, Rafaela; Barreira, João C M; Barros, Lillian; Carvalho, Ana Maria; Ferreira, Isabel C F R

    2011-04-01

    Herbal infusions and decoctions in water are some of the most commonly consumed beverages in the world. Although water is not a good solvent for many of the active components in herbs, liquid preparations are rich in several bioactive compounds. Most of them have powerful antioxidant activity and have been related to medicinal herbs' properties. Herein, decoctions and infusions in water of lemon-verbena (Aloysia citrodora) aerial parts and leaves, fennel (Foeniculum vulgare), pennyroyal (Mentha pulegium) and spearmint (Mentha spicata) aerial parts with different periods of storage (0, 30, 60 and 120 days), were prepared. The effects of the method of preparation and storage period on their antioxidant properties were analysed. For all the analysed species, infusions gave better results than the corresponding decoctions. Spearmint infusions showed the highest antioxidant properties, at all the storage periods, probably due to the highest levels and synergy between phenolics, flavonoids and ascorbic acid found in this sample. Linear discriminant analysis confirmed that the length of storage period has a significant influence on the antioxidant activity and antioxidant content. Flavonoids and reducing sugars proved to be the parameters that most highly contributed to cluster individual groups according to different periods of storage.

  20. Infusions and decoctions of mixed herbs used in folk medicine: synergism in antioxidant potential.

    Science.gov (United States)

    Guimarães, Rafaela; Barros, Lillian; Carvalho, Ana Maria; Ferreira, Isabel C F R

    2011-08-01

    Infusions (herbal teas) and decoctions are used frequently to administer oral doses of herbs. Although some herbs are used as single ingredients, they are often prepared as mixtures, as reported by numerous ethnobotanical surveys. The present work was carried out to identify the different types of interaction (synergistic, additive and antagonistic effects) which may be found in the antioxidant activity of preparations from mixtures of the popular herbs Aloysia citrodora (lemon verbena), Foeniculum vulgare (fennel) and Mentha spicata (spearmint). Herbs were prepared using traditional methods, and the effects after different periods of storage, up to 120 days, were also evaluated. Antioxidant activity was evaluated using DPPH radical scavenging activity, reducing power and inhibition of lipid peroxidation by the β-carotene - linoleate system and the TBARS assay. Known antioxidant compounds such as total phenolics, flavonoids, ascorbic acid and reducing sugars were also determined. Spearmint was found to be present in the herb mixtures with the greatest antioxidant activity and these also had the highest flavonoid content. The most potent antioxidant activity was found in combinations of different herbs, suggesting synergistic effects.