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Sample records for pigeonpea cajanus cajan

  1. Genetic patterns of domestication in pigeonpea (Cajanus cajan (L.) Millsp.) and wild Cajanus relatives.

    Science.gov (United States)

    Kassa, Mulualem T; Penmetsa, R Varma; Carrasquilla-Garcia, Noelia; Sarma, Birinchi K; Datta, Subhojit; Upadhyaya, Hari D; Varshney, Rajeev K; von Wettberg, Eric J B; Cook, Douglas R

    2012-01-01

    Pigeonpea (Cajanus cajan) is an annual or short-lived perennial food legume of acute regional importance, providing significant protein to the human diet in less developed regions of Asia and Africa. Due to its narrow genetic base, pigeonpea improvement is increasingly reliant on introgression of valuable traits from wild forms, a practice that would benefit from knowledge of its domestication history and relationships to wild species. Here we use 752 single nucleotide polymorphisms (SNPs) derived from 670 low copy orthologous genes to clarify the evolutionary history of pigeonpea (79 accessions) and its wild relatives (31 accessions). We identified three well-supported lineages that are geographically clustered and congruent with previous nuclear and plastid sequence-based phylogenies. Among all species analyzed Cajanus cajanifolius is the most probable progenitor of cultivated pigeonpea. Multiple lines of evidence suggest recent gene flow between cultivated and non-cultivated forms, as well as historical gene flow between diverged but sympatric species. Evidence supports that primary domestication occurred in India, with a second and more recent nested population bottleneck focused in tropical regions that is the likely consequence of pigeonpea breeding. We find abundant allelic variation and genetic diversity among the wild relatives, with the exception of wild species from Australia for which we report a third bottleneck unrelated to domestication within India. Domesticated C. cajan possess 75% less allelic diversity than the progenitor clade of wild Indian species, indicating a severe "domestication bottleneck" during pigeonpea domestication.

  2. Chloroplast Genome Sequence of pigeonpea (Cajanus cajan (L. Millspaugh and Cajanus scarabaeoides: Genome organization and Comparison with other legumes

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    Tanvi Kaila

    2016-12-01

    Full Text Available Pigeonpea (Cajanus cajan (L. Millspaugh, a diploid (2n = 22 legume crop with a genome size of 852 Mbp, serves as an important source of human dietary protein especially in South East Asian and African regions. In this study, the draft chloroplast genomes of Cajanus cajan and Cajanus scarabaeoides were sequenced. Cajanus scarabaeoides is an important species of the Cajanus gene pool and has also been used for developing promising CMS system by different groups. A male sterile genotype harbouring the Cajanus scarabaeoides cytoplasm was used for sequencing the plastid genome. The cp genome of Cajanus cajan is 152,242bp long, having a quadripartite structure with LSC of 83,455 bp and SSC of 17,871 bp separated by IRs of 25,398 bp. Similarly, the cp genome of Cajanus scarabaeoides is 152,201bp long, having a quadripartite structure in which IRs of 25,402 bp length separates 83,423 bp of LSC and 17,854 bp of SSC. The pigeonpea cp genome contains 116 unique genes, including 30 tRNA, 4 rRNA, 78 predicted protein coding genes and 5 pseudogenes. A 50kb inversion was observed in the LSC region of pigeonpea cp genome, consistent with other legumes. Comparison of cp genome with other legumes revealed the contraction of IR boundaries due to the absence of rps19 gene in the IR region. Chloroplast SSRs were mined and a total of 280 and 292 cpSSRs were identified in Cajanus scarabaeoides and Cajanus cajan respectively. RNA editing was observed at 37 sites in both Cajanus scarabaeoides and Cajanus cajan, with maximum occurrence in the ndh genes. The pigeonpea cp genome sequence would be beneficial in providing informative molecular markers which can be utilized for genetic diversity analysis and aid in understanding the plant systematics studies among major grain legumes.

  3. Genetic diversity in pigeonpea [Cajanus cajan (L.) Millsp ...

    African Journals Online (AJOL)

    USER

    2010-05-31

    May 31, 2010 ... India and a presumed secondary centre of diversity in East Africa were evaluated using six microsatellite .... diversity of 46 cultivated and 50 wild accessions of pigeonpea using ...... In recent years, this has been done using ...

  4. Development of genic-SSR markers by deep transcriptome sequencing in pigeonpea [Cajanus cajan (L. Millspaugh

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    Bashasab Fakrudin

    2011-01-01

    Full Text Available Abstract Background Pigeonpea [Cajanus cajan (L. Millspaugh], one of the most important food legumes of semi-arid tropical and subtropical regions, has limited genomic resources, particularly expressed sequence based (genic markers. We report a comprehensive set of validated genic simple sequence repeat (SSR markers using deep transcriptome sequencing, and its application in genetic diversity analysis and mapping. Results In this study, 43,324 transcriptome shotgun assembly unigene contigs were assembled from 1.696 million 454 GS-FLX sequence reads of separate pooled cDNA libraries prepared from leaf, root, stem and immature seed of two pigeonpea varieties, Asha and UPAS 120. A total of 3,771 genic-SSR loci, excluding homopolymeric and compound repeats, were identified; of which 2,877 PCR primer pairs were designed for marker development. Dinucleotide was the most common repeat motif with a frequency of 60.41%, followed by tri- (34.52%, hexa- (2.62%, tetra- (1.67% and pentanucleotide (0.76% repeat motifs. Primers were synthesized and tested for 772 of these loci with repeat lengths of ≥18 bp. Of these, 550 markers were validated for consistent amplification in eight diverse pigeonpea varieties; 71 were found to be polymorphic on agarose gel electrophoresis. Genetic diversity analysis was done on 22 pigeonpea varieties and eight wild species using 20 highly polymorphic genic-SSR markers. The number of alleles at these loci ranged from 4-10 and the polymorphism information content values ranged from 0.46 to 0.72. Neighbor-joining dendrogram showed distinct separation of the different groups of pigeonpea cultivars and wild species. Deep transcriptome sequencing of the two parental lines helped in silico identification of polymorphic genic-SSR loci to facilitate the rapid development of an intra-species reference genetic map, a subset of which was validated for expected allelic segregation in the reference mapping population. Conclusion We

  5. Crop wild relatives of pigeonpea [Cajanus cajan (L.) Millsp.]: Distributions, ex situ conservation status, and potential genetic resources for abiotic stress tolerance

    NARCIS (Netherlands)

    Khoury, C.K.; Castaneda-Alvarez, N.P.; Achicanoy, H.A.; Sosa, C.C.; Bernau, V.; Kassa, M.T.; Norton, S.L.; Maesen, L.; Upadhyaya, H.D.; Ramirez-Villegas, J.; Jarvis, A.; Struik, P.C.

    2015-01-01

    Pigeonpea [Cajanus cajan (L.) Millsp.] is a versatile, stress-tolerant, and nutritious grain legume, possessing traits of value for enhancing the sustainability of dry sub-tropical and tropical agricultural systems. The use of crop wild relatives (CWR) in pigeonpea breeding has been successful in pr

  6. Pigeonpea (Cajanus cajan L.) urease immobilized on glutaraldehyde-activated chitosan beads and its analytical applications.

    Science.gov (United States)

    Kayastha, A M; Srivastava, P K

    2001-01-01

    Urease from pigeonpea (Cajanus cajan L.) was covalently linked to crab shell chitosan beads using glutaraldehyde. The optimum immobilization (64% activity) was observed at 4 degrees C, with a protein concentration of 0.24 mg/bead and 3% glutaraldehyde. The immobilized enzyme stored in 0.05 M Tris-acetate buffer, pH 7.3, at 4 degrees C had a t(1/2) of 110 d. There was practically no leaching of enzyme (chitosan-immobilized urease showed a significantly higher Michaelis constant (8.3 mM) compared to that of the soluble urease (3.0 mM). Its apparent optimum pH also shifted from 7.3 to 8.5. Immobilized urease showed an optimal temperature of 77 degrees C, compared with 47 degrees C for the soluble urease. Time-dependent kinetics of the thermal denaturation of immobilized urease was studied and found to be monophasic in nature compared to biphasic in nature for soluble enzyme. This immobilized urease was used to analyze blood urea of some of the clinical samples from the clinical pathology laboratories. The results compared favorably with those obtained by the various chemical/biochemical methods employed in the clinical pathology laboratories. A column packed with immobilized urease beads was also prepared in a syringe for the regular and continuous monitoring of serum urea concentrations.

  7. Modifications on leaf anatomy of Coffea arabica caused by shade of pigeonpea (Cajanus cajan

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    Heverly Morais

    2004-11-01

    Full Text Available Modifications on leaf anatomy in Coffea arabica shaded with pigeonpea (Cajanus cajan, compared to cultivation under full sun, were assessed. The leaves fully exposed to sunlight presented thicker cuticles and cellular walls, narrower epidermis cells, palisade parenchyma with longer cells, thicker lacunar parenchyma, fewer intercellular spaces and a larger stomata number. Leaves under dense shade presented a narrower cuticle and cellular wall; a mesophyll with smaller volume, but with larger intercellular spaces; and epidermis with thicker cells and a smaller stomata amount, surrounded by subsidiary cells of smaller dimensions. Plants grown under full sunlight presented higher values of net photosynthesis. The results evidenced that the species C. arabica has a wide range of phenotypic adaptation to changes in the radiation intensity.Adaptações de plantas da mesma espécie aos diferentes habitats, constituem a base da diferenciação entre folhas a pleno sol e folhas sob sombra e estão associadas a características anatômicas distintas. Para caracterizar tais mudanças em cafeeiros cultivados sob sombra de guandu (Cajanus cajan e a pleno sol, em Londrina, PR, foram realizadas avaliações de modificações ocorridas na anatomia foliar. As estruturas internas avaliadas foram: parede celular e cutícula; epiderme; mesofilo (parênquima paliçádico, parênquima lacunoso e espaços intercelulares e estômatos. Para todas as variáveis avaliadas observaram-se diferenças anatômicas entre folhas expostas ao sol e à sombra. As folhas expostas ao sol apresentaram cutículas e paredes celulares mais espessas, células da epiderme mais estreitas, parênquima paliçádico com células mais alongadas, parênquima lacunoso espesso e com poucos espaços intercelulares e maior número de estômatos. Folhas sob condições de denso sombreamento apresentam menor espessamento da cutícula e da parede celular; mesofilos com menores volumes, porém com

  8. The first set of EST resource for gene discovery and marker development in pigeonpea (Cajanus cajan L.

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    Byregowda Munishamappa

    2010-03-01

    Full Text Available Abstract Background Pigeonpea (Cajanus cajan (L. Millsp is one of the major grain legume crops of the tropics and subtropics, but biotic stresses [Fusarium wilt (FW, sterility mosaic disease (SMD, etc.] are serious challenges for sustainable crop production. Modern genomic tools such as molecular markers and candidate genes associated with resistance to these stresses offer the possibility of facilitating pigeonpea breeding for improving biotic stress resistance. Availability of limited genomic resources, however, is a serious bottleneck to undertake molecular breeding in pigeonpea to develop superior genotypes with enhanced resistance to above mentioned biotic stresses. With an objective of enhancing genomic resources in pigeonpea, this study reports generation and analysis of comprehensive resource of FW- and SMD- responsive expressed sequence tags (ESTs. Results A total of 16 cDNA libraries were constructed from four pigeonpea genotypes that are resistant and susceptible to FW ('ICPL 20102' and 'ICP 2376' and SMD ('ICP 7035' and 'TTB 7' and a total of 9,888 (9,468 high quality ESTs were generated and deposited in dbEST of GenBank under accession numbers GR463974 to GR473857 and GR958228 to GR958231. Clustering and assembly analyses of these ESTs resulted into 4,557 unique sequences (unigenes including 697 contigs and 3,860 singletons. BLASTN analysis of 4,557 unigenes showed a significant identity with ESTs of different legumes (23.2-60.3%, rice (28.3%, Arabidopsis (33.7% and poplar (35.4%. As expected, pigeonpea ESTs are more closely related to soybean (60.3% and cowpea ESTs (43.6% than other plant ESTs. Similarly, BLASTX similarity results showed that only 1,603 (35.1% out of 4,557 total unigenes correspond to known proteins in the UniProt database (≤ 1E-08. Functional categorization of the annotated unigenes sequences showed that 153 (3.3% genes were assigned to cellular component category, 132 (2.8% to biological process, and 132 (2

  9. Genomics-assisted breeding for boosting crop improvement in pigeonpea (Cajanus cajan

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    Lekha ePazhamala

    2015-02-01

    Full Text Available Pigeonpea is an important pulse crop grown predominantly in the tropical and sub-tropical regions of the world. Although pigeonpea growing area has considerably increased, yield has remained stagnant for the last six decades mainly due to the exposure of the crop to various biotic and abiotic constraints. In addition, low level of genetic variability and limited genomic resources have been serious impediments to pigeonpea crop improvement through modern breeding approaches. In recent years, however, due to the availability of next generation sequencing and high-throughput genotyping technologies, the scenario has changed tremendously. The reduced sequencing costs resulting in the decoding of the pigeonpea genome has led to the development of various genomic resources including molecular markers, transcript sequences and comprehensive genetic maps. Mapping of some important traits including resistance to Fusarium wilt and sterility mosaic disease, fertility restoration, determinacy with other agronomically important traits have paved the way for applying genomics-assisted breeding (GAB through marker assisted selection as well as genomic selection. This would lead to accelerate the development and improvement of both varieties and hybrids in pigeonpea. Particularly for hybrid breeding programme, mitochondrial genomes of cytoplasmic male sterile lines, maintainers and hybrids have also been sequenced to identify genes responsible for cytoplasmic male sterility. Furthermore, several diagnostic molecular markers have been developed to assess the purity of commercial hybrids. In summary, pigeonpea has become a genomic resources-rich crop and efforts have already been initiated to integrate these resources in pigeonpea breeding.

  10. Identification and validation of selected universal stress protein domain containing drought-responsive genes in pigeonpea (Cajanus cajan L.

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    Pallavi eSinha

    2016-01-01

    Full Text Available Pigeonpea is a resilient crop, which is relatively more drought tolerant than many other legume crops. To understand the molecular mechanisms of this unique feature of pigeonpea, 51 genes were selected using the Hidden Markov Models those codes for proteins having close similarity to universal stress protein domain. Validation of these genes was conducted on three pigeonpea genotypes (ICPL 151, ICPL 8755 and ICPL 227 having different levels of drought tolerance. Gene expression analysis using qRT-PCR revealed 6, 8 and 18 genes to be ≥2 fold differentially expressed in ICPL 151, ICPL 8755 and ICPL 227, respectively. A total of 10 differentially expressed genes showed ≥2 fold up-regulation in the more drought tolerant genotype. Of these, four genes each encoded proteins for plant U-box and universal stress protein A- (uspA like, while one gene encoded for cation/H(+ antiporter protein and one uncharacterized protein. Genes C.cajan_29830 and C.cajan_33874 belonging to uspA, were found significantly expressed in all the three genotypes with ≥2 fold expression variations. Expression profiling of these two genes on the four other legume crops revealed their specific role in pigeonpea. Therefore, these genes seem to be promising candidates for conferring drought tolerance specifically to pigeonpea.

  11. Proteomic analysis of Pigeonpea (cajanus cajan) seeds reveals the accumulation of numerous stress-related proteins

    Science.gov (United States)

    Pigeonpea is one of the major sources of dietary protein for more than a billion people living in South Asia. This hardy legume is often grown in low-input and risk-prone marginal environments. Considerable research effort has been devoted by a global research consortium to develop genomic resources...

  12. Deciphering Transcriptional Programming during Pod and Seed Development Using RNA-Seq in Pigeonpea (Cajanus cajan)

    Science.gov (United States)

    Pazhamala, Lekha T.; Agarwal, Gaurav; Bajaj, Prasad; Kumar, Vinay; Kulshreshtha, Akanksha; Saxena, Rachit K.; Varshney, Rajeev K.

    2016-01-01

    Seed development is an important event in plant life cycle that has interested humankind since ages, especially in crops of economic importance. Pigeonpea is an important grain legume of the semi-arid tropics, used mainly for its protein rich seeds. In order to understand the transcriptional programming during the pod and seed development, RNA-seq data was generated from embryo sac from the day of anthesis (0 DAA), seed and pod wall (5, 10, 20 and 30 DAA) of pigeonpea variety “Asha” (ICPL 87119) using Illumina HiSeq 2500. About 684 million sequencing reads have been generated from nine samples, which resulted in the identification of 27,441 expressed genes after sequence analysis. These genes have been studied for their differentially expression, co-expression, temporal and spatial gene expression. We have also used the RNA-seq data to identify important seed-specific transcription factors, biological processes and associated pathways during seed development process in pigeonpea. The comprehensive gene expression study from flowering to mature pod development in pigeonpea would be crucial in identifying candidate genes involved in seed traits directly or indirectly related to yield and quality. The dataset will serve as an important resource for gene discovery and deciphering the molecular mechanisms underlying various seed related traits. PMID:27760186

  13. Estimation of Heterosis, Combining ability and Gene action in Pigeonpea [Cajanus cajan (L. Millsp.

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    Yamanura,, *Lokesha R., Dharmaraj, P.S., Muniswamy, S. and Diwan, J.R.

    2014-06-01

    Full Text Available Eight cytoplasmic-genetic male sterile lines with A2 (Cajanus scarabaeoides and A4 (Cajanus cajanifolius cytoplasm were crossed with 10 testers in a line × tester design. Resultant 80 experimental hybrids were evaluated along with parents and check (Maruti in lattice design with two replications. Combining ability analysis evinced predominance of non-additive gene effects for 10 characters indicating relevance of heterosis breeding for improving yield attributes. The gca effects of parents revealed that ICPA-2078, GT-308A, PKV-TATA, ARCCV-2 and GPHR-08-11 were good general combiners for seed yield and its direct components. The estimates of sca effects revealed that nine experimental hybrids had significant, desirable and positive sca effects for seed yield. The cross combination ICPA-2092 x VIPUL, ICPA-2078 x BSMR-856 and ICPA-2078 x ARCCV-2 were good specific combiners for number of secondary branches, number of pods per plant, seed yield per plant. These parental combinations are being used for exploitation of hybrid vigour.

  14. A Comprehensive Transcriptome Assembly of Pigeonpea (Cajanus cajan L.) using Sanger and Second-Generation Sequencing Platforms

    Science.gov (United States)

    Kudapa, Himabindu; Bharti, Arvind K.; Cannon, Steven B.; Farmer, Andrew D.; Mulaosmanovic, Benjamin; Kramer, Robin; Bohra, Abhishek; Weeks, Nathan T.; Crow, John A.; Tuteja, Reetu; Shah, Trushar; Dutta, Sutapa; Gupta, Deepak K.; Singh, Archana; Gaikwad, Kishor; Sharma, Tilak R.; May, Gregory D.; Singh, Nagendra K.; Varshney, Rajeev K.

    2012-01-01

    A comprehensive transcriptome assembly for pigeonpea has been developed by analyzing 128.9 million short Illumina GA IIx single end reads, 2.19 million single end FLX/454 reads, and 18 353 Sanger expressed sequenced tags from more than 16 genotypes. The resultant transcriptome assembly, referred to as CcTA v2, comprised 21 434 transcript assembly contigs (TACs) with an N50 of 1510 bp, the largest one being ∼8 kb. Of the 21 434 TACs, 16 622 (77.5%) could be mapped on to the soybean genome build 1.0.9 under fairly stringent alignment parameters. Based on knowledge of intron junctions, 10 009 primer pairs were designed from 5033 TACs for amplifying intron spanning regions (ISRs). By using in silico mapping of BAC-end-derived SSR loci of pigeonpea on the soybean genome as a reference, putative mapping positions at the chromosome level were predicted for 6284 ISR markers, covering all 11 pigeonpea chromosomes. A subset of 128 ISR markers were analyzed on a set of eight genotypes. While 116 markers were validated, 70 markers showed one to three alleles, with an average of 0.16 polymorphism information content (PIC) value. In summary, the CcTA v2 transcript assembly and ISR markers will serve as a useful resource to accelerate genetic research and breeding applications in pigeonpea. PMID:22241453

  15. Draft genome sequence of pigeonpea (Cajanus cajan), an orphan legume crop of resource-poor farmers

    DEFF Research Database (Denmark)

    Varshney, Rajeev K.; Chen, Wenbin; Li, Yupeng;

    2012-01-01

    Pigeonpea is an important legume food crop grown primarily by smallholder farmers in many semi-arid tropical regions of the world. We used the Illumina next-generation sequencing platform to generate 237.2 Gb of sequence, which along with Sanger-based bacterial artificial chromosome end sequences...

  16. A Comprehensive Transcriptome Assembly of Pigeonpea (Cajanus cajan L.) using Sanger and Second-Generation Sequencing Platforms

    Institute of Scientific and Technical Information of China (English)

    Himabindu Kudapa; Reetu Tuteja; Trushar Shah; Sutapa Dutta; Deepak K.Gupta; Archana Singh; Kishor Gaikwad; Tilak R.Sharma; Gregory D.May; Nagendra K.Singh; Rajeev K.Varshney; Arvind K.Bharti; Steven B.Cannon; Andrew D.Farmer; Benjamin Mulaosmanovic; Robin Kramer; Abhishek Bohra; Nathan T.Weeks; John A.Crow

    2012-01-01

    A comprehensive transcriptome assembly for pigeonpea has been developed by analyzing 128.9 million short Illumina GA Ⅱx single end reads,2.19 million single end FLX/454 reads,and 18353 Sanger expressed sequenced tags from more than 16 genotypes.The resultant transcriptome assembly,referred to as CcTA v2,comprised 21434 transcript assembly contigs (TACs) with an N50 of 1510 bp,the largest one being ~8 kb.Of the 21434 TACs,16622 (77.5%) could be mapped on to the soybean genome build 1.0.9 under fairly stringent alignment parameters.Based on knowledge of intron junctions,10009 primer pairs were designed from 5033 TACs for amplifying intron spanning regions (ISRs).By using in silico mapping of BAC-end-derived SSR loci of pigeonpea on the soybean genome as a reference,putative mapping positions at the chromosome level were predicted for 6284 ISR markers,covering all 11 pigeonpea chromosomes.A subset of 128 ISR markers were analyzed on a set of eight genotypes.While 116 markers were validated,70 markers showed one to three alleles,with an average of 0.16 polymorphism information content (PIC) value.In summary,the CcTA v2 transcript assembly and ISR markers will serve as a useful resource to accelerate genetic research and breeding applications in pigeonpea.

  17. Genetic diversity studies and identification of SSR markers associated with Fusarium wilt (Fusarium udum) resistance in cultivated pigeonpea (Cajanus cajan)

    Indian Academy of Sciences (India)

    A. K. Singh; V. P. Rai; R. Chand; R. P. Singh; M. N. Singh

    2013-08-01

    Genetic diversity and identification of simple sequence repeat markers correlated with Fusarium wilt resistance was performed in a set of 36 elite cultivated pigeonpea genotypes differing in levels of resistance to Fusarium wilt. Twenty-four polymorphic sequence repeat markers were screened across these genotypes, and amplified a total of 59 alleles with an average high polymorphic information content value of 0.52. Cluster analysis, done by UPGMA and PCA, grouped the 36 pigeonpea genotypes into two main clusters according to their Fusarium wilt reaction. Based on the Kruskal–Wallis ANOVA and simple regression analysis, six simple sequence repeat markers were found to be significantly associated with Fusarium wilt resistance. The phenotypic variation explained by these markers ranged from 23.7 to 56.4%. The present study helps in finding out feasibility of prescreened SSR markers to be used in genetic diversity analysis and their potential association with disease resistance.

  18. New Hypervariable SSR Markers for Diversity Analysis, Hybrid Purity Testing and Trait Mapping in Pigeonpea [Cajanus cajan (L.) Millspaugh].

    Science.gov (United States)

    Bohra, Abhishek; Jha, Rintu; Pandey, Gaurav; Patil, Prakash G; Saxena, Rachit K; Singh, Indra P; Singh, D; Mishra, R K; Mishra, Ankita; Singh, F; Varshney, Rajeev K; Singh, N P

    2017-01-01

    Draft genome sequence in pigeonpea offers unprecedented opportunities for genomics assisted crop improvement via enabling access to genome-wide genetic markers. In the present study, 421 hypervariable simple sequence repeat (SSR) markers from the pigeonpea genome were screened on a panel of eight pigeonpea genotypes yielding marker validation and polymorphism percentages of 95.24 and 54.11%, respectively. The SSR marker assay uncovered a total of 570 alleles with three as an average number of alleles per marker. Similarly, the mean values for gene diversity and PIC were 0.44 and 0.37, respectively. The number of polymorphic markers ranged from 39 to 89 for different parental combinations. Further, 60 of these SSRs were assayed on 94 genotypes, and model based clustering using STRUCTURE resulted in the identification of the two subpopulations (K = 2). This remained in close agreement with the clustering patterns inferred from genetic distance (GD)-based approaches i.e., dendrogram, factorial and principal coordinate analysis (PCoA). The AMOVA accounted majority of the genetic variation within groups (89%) in comparison to the variation existing between the groups (11%). A subset of these markers was implicated for hybrid purity testing. We also demonstrated utility of these SSR markers in trait mapping through association and bi-parental linkage analyses. The general linear (GLM) and mixed linear (MLM) models both detected a single SSR marker (CcGM03681) with R(2) = 16.4 as associated with the resistance to Fusarium wilt variant 2. Similarly, by using SSR data in a segregating backcross population, the corresponding restorer-of-fertility (Rf) locus was putatively mapped at 39 cM with the marker CcGM08896. However, The marker-trait associations (MTAs) detected here represent a very preliminary type and hence demand deeper investigations for conclusive evidence. Given their ability to reveal polymorphism in simple agarose gels, the hypervariable SSRs are valuable

  19. Unexpected effects of pigeon-peas (Cajanus cajan in the restoration of rupestrian fields Efeito inesperado do feijão-guandu (Cajanus cajan na restauração de campos rupestres

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    R.R Hilário

    2011-12-01

    Full Text Available Several degraded areas can be found along the Highway MG-010 that crosses the Espinhaço Mountain Biosphere Reserve in the Brazilian state of Minas Gerais. Restoration by planting the legume Cajanus cajan was implemented in some of these areas. The present study compares plant species richness, diversity, abundance, equitability, similarity, and soil composition between restored and non-restored areas, in an attempt to evaluate the effectiveness of the use of C. cajan in the restoration process in the mountain environment. Each treatment (restored and non-restored had four sampling areas, each with three 300 m² plots. We counted and identified every individual plant found within these plots. We also collected soil from the superficial layer (0-10 cm of each sampling area in both treatments. The areas where C. cajan was planted revealed lower species richness, diversity, and plant abundance. The soil of these areas also contained higher levels of Phosphorus and Magnesium. Plant equitability and similarity between plots and other soil components (pH, Nitrogen, Aluminum, Calcium, Potassium, H+Al, sum of bases - SB, cation exchange capacity - CTC, base saturation - V%, aluminum saturation - M% did not differ between the two treatments. Contrary to the expectations, soil enhancement in the quartzitic soil poor in nutrients in the rupestrian fields can facilitate the invasion by exotic plants, which are not adapted to the lack of nutrients. As it appears, the use of C. cajan in restoration projects represents a mistake and future restoration plans should avoid the use of exotic species, given that they may cause negative effects on the native plant community, as demonstrated here in the rupestrian fields.Várias áreas degradadas podem ser encontradas ao longo da rodovia MG-010, que corta a Reserva da Biosfera da Cadeia do Espinhaço, em Minas Gerais, Brasil. Algumas dessas áreas foram restauradas através do plantio da leguminosa Cajanus cajan. O

  20. Analysis of toxic elements in two pigeonpea (Cajanus cajan (L.) Millsp) cultivars, in fertilized soils, by neutron activation; Analise de elementos toxicos em duas variedades de guandu (Cajanus cajan (L.) Millsp), cultivadas em solos tratados, por ativacao com neutrons

    Energy Technology Data Exchange (ETDEWEB)

    Piasentin, Ricardo M.; Armelin, Maria Jose A. [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil). Divisao de Radioquimica; Primavesi, Odo [Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA), Sao Carlos, SP (Brazil). Centro de Pesquisa de Pecuaria do Sudeste

    2000-07-01

    Samples of whole leaves, from thirty-six plants belonging to two pigeonpea cultivars were analysed by Instrumental Neutron Activation Analysis. Each plant was cultivated under either single dose or double one of some mineral fertilizers, such as, B, Co, Cu, Fe, Mn, Mo, V and Zn, individually, to the soil; besides limestone and phosphorus. The aim of this paper is to evaluate the contribution of these treatments to the increase in the concentrations of As, Sb, Th and U, since these elements can be toxic to plants and animals. (author)

  1. Avaliação agronômica de linhagens selecionadas de guandu (Cajanus cajan (L. Millsp Agronomic evaluation of selected pigeon-pea lines (Cajanus cajan (L. Millsp

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    Rodolfo Godoy

    2005-02-01

    Full Text Available Com o objetivo de reavaliar agronomicamente 17 linhagens de guandu, originárias de acessos anteriormente selecionados por diversas características de interesse agronômico, foram instalados experimentos em cinco locais do Estado de São Paulo. A confirmação dessas características foi necessária, uma vez que as amostras de sementes dos acessos originais apresentavam mistura mecânica e segregação, razão pela qual, após serem selecionados, os acessos passaram por processo de purificação em condições controladas de polinização. Nesses experimentos, as linhagens foram submetidas a cortes para avaliação da produção de forragem, ocasião em que foram determinados o número de plantas na área útil da parcela e a altura média dessas plantas. Foi também determinado o teor de proteína bruta e de taninos de amostras da forragem produzida. Entre as linhagens testadas, g58-95 e g127-97 confirmaram ter plantas de baixa estatura, g3-94, g167-97 e g29b-94 confirmaram oferecer boas produções de forragem e g146-97 destacou-se quanto à produção inicial de matéria seca.With the purpose of re-evaluating seventeen pigeon-pea lines originated from accessions previously selected for favorable agronomic characteristics, experiments were installed in five locations of the State of São Paulo. The confirmation of those characteristics was necessary because the original accessions presented various degrees of mechanical mixtures and segregation and were submitted to purification process in pollination controlled conditions. In those trials, the lines were cut in several occasions to evaluate forage yield. Each time, it was determined the number of plants in each parcel and its average height. Also it was determined their crude protein and tannins contents. Among the tested lines, g58-95 e g127-97 confirmed to have low plants, g94, g167-97 and g29b-94, confirmed to offer good forage yields, while line g146-97 presented good initial forage

  2. Caractérisation du savoir paysan sur les insectes nuisibles du pois d'Angole (Cajanus Cajan (L. Millsp. dans le Nord de l'Ouganda

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    Velay F.

    2001-01-01

    Full Text Available Characterization of indigenous traditional knowledge on insect pests of pigeonpea (Cajanus cajan (L. Millsp. in Northern Uganda. In order to assess the perception of the main insect pests of pigeonpea (Cajanus cajan (L. Millsp. by small farmers and to inventoriate methods traditionally used to control these insects, a survey was conducted in 41 households of Northern Uganda (Lira and Apac districts. Virtually all small farmers (95/ considered blister beetle (Mylabris sp., which is the most conspicious insect damaging pigeonpea at the flowering stage, as the main pest of the crop. This is conflicting with field observations made earlier in Northern Uganda which pointed out pod borers (Helicoverpa armigera Huebner and Maruca vitrata Geyer and pod sucking bugs (mainly Clavigralla sp. as the most damaging pests for pigeonpea production. A new pest of pigeonpea, Luprops baldius Mueller, was identified during this survey. Its adults and larvae are damaging to mature pods in case of late harvest.

  3. Introgression of Helicoverpa armigera Resistance from Cajanus acutifolius-a Wild Relative from Secondary Gene Pool of Pigeon Pea (Cajanus cajan

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    Deepak R. Jadhav

    2012-07-01

    Full Text Available The aim of the study was to introgress Helicoverpa armigera resistance from wild relative Cajanus acutifolius into pigeonpea, (Cajanus cajan L., an important grain legume in South Asia, East Africa and the West Indies. Pigeonpea grain yields on farmer’s fields are quite low, largely because of damage by insect pests, of which legume pod borer Helicoverpa armigera (Hübner (Lepidoptera: Noctuidae is the important pest worldwide. Pod borer has developed high levels of resistance to chemical insecticides. Currently, there are no cultivars of pigeonpea with high levels of resistance to H. armigera. Therefore, there is a need to identify and introgress resistance genes from the wild relatives of this crop. Wild relative of pigeonpea, Cajanus acutifolius (ICPW 15613 and the interspecific derivatives C. acutifolius x C. cajan have shown resistance to H. armigera. The results showed that all the test lines and C. acutifolius had high levels of flavonoids such as chlorogenic acid, quercetin and rutin in the flowers and buds, which may have resulted in less damage due to H. armigera larvae. Most of the test lines had more than 15.00 g of seed weight (100 seed weight and beige seed color. These lines can be used for pigeonpea improvement for resistance to H. armigera.

  4. Novel CMS lines in pigeonpea [Cajanus cajan (L. Millspaugh] derived from cytoplasmic substitutions, and their effective restoration and deployment in hybrid breeding

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    Abhishek Bohra

    2017-02-01

    Full Text Available The availability of stable cytoplasmic male sterile (CMS or A lines coupled with a robust restoration system (R lines is an essential prerequisite for efficient hybrid breeding. CMS-enabled hybrid technology holds immense potential to enhance the long-stagnant productivity of pigeonpea. In the present investigation, cytoplasmic substitutions were made in the nuclear backgrounds of early-maturing pigeonpea varieties or lines. Three new CMS lines (ICPL 88039A, Pusa 992A, and DPP 3-2A resulted from genetic crosses involving cytoplasmic donors from A2 (GT 288A and A4 (ICPA 2089 categories. In addition to visual inspection of anthers, pollen-staining techniques and scanning electron microscopy (SEM analysis were used to confirm pollen sterility. Further, given the relevance of the plant mitochondrial genome to CMS manifestation, 25 mitochondrion-specific DNA markers were assayed on these newly developed A lines and isogenic maintainer (B lines. DNA polymorphism between Pusa 992A and Pusa 992B as revealed by the nad7a_del marker confirmed the successful combination of sterilizing cytoplasm (A4 and nonrestoring nuclear background (Pusa 992. Such cytoplasm-specific DNA markers are required for A2-CMS as well. Further, to assess restoration ability, potential restorers were crossed with these CMS lines, and as a consequence, promising A × R combinations exhibiting 100% pollen fertility could be identified. In parallel, we also analyzed the inheritance patterns underlying fertility restoration using ICPL 88039A-derived F2 and BC1F1 populations, and established a monogenic dominant model to explain the phenomenon of A2-CMS restoration. In summary, we report the successful development of new CMS lines and describe their effective deployment in hybrid breeding of pigeonpea.

  5. Biological activities and medicinal properties of Cajanus cajan (L Millsp.

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    Dilipkumar Pal

    2011-01-01

    Full Text Available Cajanus cajan (L Millsp. (Sanskrit: Adhaki, Hindi: Arhar, English: Pigeon pea, Bengali: Tur (family: Fabaceae is the most important grain legume crop of rain-fed agriculture in semi-arid tropics. It is both a food crop and a cover/forage crop with high levels of proteins and important amino acids like methionine, lysine and tryptophan. During the last few decades extensive studies have been carried out regarding the chemistry of C. cajan and considerable progress has been achieved regarding its biological activities and medicinal applications. This review article gives an overview on the biological activities of the compounds isolated, pharmacological actions and clinical studies of C. cajan extracts apart from its general details.

  6. Chemical and pharmacological characterization of hypolipidemic compound from Cajanus Cajan

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    Md. Anwar Habib

    2010-03-01

    Full Text Available The study was carried out to identify the compound responsible for hypolipidemic and hypoglycemic effects of Cajanus cajan (redgram. The methanol extract of redgram seeds were fractionated into petroleum ether, chloroform, and methanol. The methanol fraction significantly decreased fasting blood glucose, and lipid profiles (p<0.001 on streptozotocin-induced mice compared to control. The methanol fraction was then subjected to chromatographic analysis and a compound (CCA1 has been isolated. The structure of the compound is considered to be substituted cyclopentene with glucose by analysis its 1H and 13C-NMR data. Biological studies of the isolated compound possessed prominent hypolipidemic activity. Although a number of hypoglycemic compounds are reported, yet not any hypolipidemic compound from redgram. The compound CCA1 seems to be the first report on hypolipidemic activity from methanol extract of redgram.

  7. PIGEON PEA (Cajanus cajan AN ALTERNATIVE IN THE FOOD INDUSTRY

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    Carmen Lucia Navarro V

    2014-12-01

    Full Text Available Due to the current situation of inadequate nutrition in the population of many countries, including Colombia. Search sources rich in proteins and low-cost alternatives. The pigeon pea (Cajanus cajan is an important legume that contain a mo derate amount of protein, calories, vitamins and minerals, its use in foods is limited by the presence of anti-nutritional factors, which can be reduced or eliminated through the use of treatments. The proteins have functional properties that can be take advantage in meat, dairy and bakery products. The purpose of this review is to present an overview of the skills nutritional and functional properties of pigeon pea application opportunities in various applications in the food industry.

  8. Molecular diversity among wild relatives of Cajanus cajan (L.) Millsp.

    African Journals Online (AJOL)

    Breeding, Pigeonpea (ICRISAT-N)

    In pigeonpea germplasm, a large phenotypic va- ... genetic variation available in the related wild species. To ... pigeonpea played a major role in the development of ..... van der Maesen LJG (1990) Pigeonpea: origin, history, evolution and.

  9. Changes and induction of aminopeptidase activities in response to pathogen infection during germination of pigeonpea (Cajanas cajan) seeds.

    Science.gov (United States)

    Lomate, Purushottam R; Hivrale, Vandana K

    2011-10-15

    Aminopeptidases play important role in the mobilization of storage proteins at the cotyledon during seed germination. It is often referred as inducible component of defense against herbivore attack. However the role of aminopeptidase in response to pathogen attack in germinating seeds is remained to be unknown. An attempt was made to analyze change in the aminopeptidase (EC 3.4.11.1) activity during germination of pigeonpea (Cajanus cajan L.) seeds by infecting the seeds with fungi. Two aminopeptidase activity bands (AP1 and AP2) were detected in control as well as infected pigeonpea seeds. During latter stages of germination in control seeds, AP1 activity was replaced by AP2 activity. However AP1 activity was significantly induced in germinating seeds infected with Fusarium oxysporum f.sp. ciceri and Aspergillus niger var. niger. The estimated molecular weights of AP1 and AP2 were ∼97 and 42.8kDa respectively. The induced enzyme was purified up to 30 fold by gel filtration chromatography. The purified enzyme was preferentially cleaved leucine p-nitroanilide than alanine p-nitroanilide. The enzyme was strongly inhibited by bestatin and 1,10-phenanthroline. Almost 50% of enzyme activity was inhibited by ethylene diamine tetra acetate. The purified enzyme showed broad pH optima ranging from pH 6.0 to 9.0 and optimum at pH 8.5. The induction of aminopeptidase activity during pigeonpea seed germination and in response to pathogen attack indicates significant involvement of these enzymes in primary as well as secondary metabolism of the seeds. These findings could be helpful to further dissect defensive role of aminopeptidases in seed germination which is an important event in plant's life. Copyright © 2011 Elsevier GmbH. All rights reserved.

  10. Guandu bean (Cajanus cajan (L. Millsp. on tropical forest restoration/ Feijão guandu (Cajanus cajan (L. Millsp. na restauração de florestas tropicais

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    Efraim Rodrigues

    2007-07-01

    Full Text Available This work aims to evaluate alternative models in forest restorations by means of agroforestry systems, where a leguminous species is incorporated to the system. The hypothesis is that guandu bean Cajanus cajan (L. Millsp., in combination and intercropped with native species, can promote restoration by decreasing mortality and increasing tree basal area and height. Cajanus cajan was planted in line with the forest seedlings in 2 x 4 m spacing. Four different treatments were analyzed; 1 control, with no Cajanus cajan ; 2 one seedling of Cajanus cajan between the forest seedlings in the plantation line; 3 two seedlings of Cajanus cajan between the forest seedlings in the plantation line; and 4 full harvest Cajanus cajan six months after plot implementation. In each treatment, 100 native trees (Fifty pioneer, and fifty non pioneer trees were measured and evaluated in mortality rates, height, and basal diameter. Guandu planting reduced pioneer mortality, increased basal area, and tree height of all species. In spite of the mortality was not affected by guandu density, the density of one plant of guandu was associated with larger basal area and tree average height. The mortality reduction of non pioneers on the absence of guandu trees was interpreted as a response of antropic pioneers to the increased solar radiation. This result points to the differences in tree behavior between fertile gaps (on which the classification on sucessional stages was based and degraded areas. The positive effects of guandu planting recommend its use for the ecological restoration cost reduction.O objetivo deste trabalho é avaliar formas alternativas de restauração de áreas degradadas, utilizando sistemas agroflorestais com uma espécie leguminosa incorporada ao sistema. A hipótese é que o feijão guandu (Cajanus cajan (L. Millsp. possa auxiliar o processo de restauração, diminuindo a mortalidade e aumentando a altura e área basal das árvores. O feijão guandu

  11. Antioxidant status of pigeon pea, Cajanus cajan in the presence of endosulfan stress.

    Science.gov (United States)

    Mathad, Pratima; Siddaling, N C

    2009-05-01

    Antioxidative status study was made in cotyledons of 7days old as well as in leaf and stem tissues of 30 and 60 days old pigeon pea (Cajanus cajan) namely Asha and Maruti subjected to different doses of endosulfan in the range 0.1-1.0%. The results revealed that the activities of the antioxidative enzymes and the antioxidant contents such as the super oxide dismutase (SOD), peroxidase (POD), reducing power (RP), ascorbic acid (AsA) and total phenols (TP) increased with increase in the concentrations of endosulfan in different parts of the plants in both the varieties. It was interesting to note that the increase in the antioxidative enzymes and the antioxidant contents were higher in leaves than in stem and cotyledons in both the plant varieties. The Asha showed lower activity of SOD and higher activity of POD than the Maruti. The RP and AsA contents were higher whereas the TP content was lower in Asha than Maruti. The observed variations in the activities of the oxidative enzymes and the antioxidant contents of the plants treated with the varying concentration of endosulfan indicated that the antioxidative system in the plants plays a fundamental role in minimizing the deleterious effects of the oxidative stress in the two varieties of Cajanus cajan.

  12. In vitro antioxidant and pharmacognostic studies of leaf extracts of cajanus cajan (l. millsp

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    B Mahitha

    2015-01-01

    Full Text Available Cajanus cajan (L. Millsp is one of the second most dietary legume crops. The leaf extracts may be used as a potential source of natural antioxidant. The ash values, extractive values, total phenolic and flavonoid content, in vitro antioxidant activity of various leaf extracts as well as anatomical investigation of Cajanus cajan were carried out. Physicochemical parameters such as total, acid-insoluble and water-soluble ash values and moisture content of the leaf powder of C. cajan were found to be 9.50%, 1.40 g/100 g, 4.15 g/100 g drug and 6.72%, respectively. Percent yield of acetone, aqueous, ethanol, ethyl acetate and chloroform leaf extracts were 9.0, 10.6, 13.75, 8.7 and 5.8 g/100 g, respectively. Significant amount of phenolic and flavonoid content were observed. The results of the antioxidant activity were found to be concentration-dependent. The IC 50 values for DPPH assay determined for aqueous and ethanol extracts were 0.69 and 0.79 mg/ml, respectively. Reducing power is increased with increasing amount of concentration in both aqueous and ethanol leaf extracts. The highest hydroxyl radical scavenging activity reached up to 83.67% in aqueous and 78.75% in ethanol extracts and in phosphomolybdenum assay the aqueous extract showed strong antioxidant capacity up to 55.97 nM gallic acid equivalents/g. It was found that the aqueous extract possessed highest antioxidant activity in all the assays tested.The antioxidant characteristics of leaf extracts are possibly because of the presence of polyphenols. Microscopic study showed the presence of collenchyma, fibres, xylem, phloem, epidermis, trichomes, palisade tissue, basal sheath, pith and cortex in leaf, petiole and pulvinus.

  13. Evaluation of Cajanus cajan (pigeon pea) for phytoremediation of landfill leachate containing chromium and lead.

    Science.gov (United States)

    Jerez Ch, José A; Romero, Rosaura M

    2016-11-01

    Landfill leachates containing heavy metals are important contaminants and a matter of great concern due to the effect that they might have on ecosystems. We evaluated the use of Cajanus cajan to remove chromium and lead from landfill leachates. Eight-week-old plants were submitted to varied tests to select the experimental conditions. Water assays with a solution (pH 6) containing leachate (25% v/v) were selected; the metals were added as potassium dichromate and lead (II) nitrate salts. Soil matrices that contained leachate (30% v/v) up to field capacity were used. For both water and soil assays, the metal concentrations were 10 mg kg(-1). C. cajan proved able to remove 49% of chromium and 36% of lead, both from dilute leachate. The plants also removed 34.7% of chromium from irrigated soil, but were unable to decrease the lead content. Removal of nitrogen from landfill leachate was also tested, resulting in elimination of 85% of ammonia and 70% of combined nitrite/nitrate species. The results indicate that C. cajan might be an effective candidate for the rhizofiltration of leachates containing chromium and lead, and nitrogen in large concentrations.

  14. Antifungal potential of some higher plants against Fusarium udum causing wilt disease of Cajanus cajan.

    Science.gov (United States)

    Singh, R; Rai, B

    2000-01-01

    The fungitoxic effects of different plant extracts on Fusarium udum, which causes wilt disease of Cajanus cajan in vitro and in vivo, were examined. The complete arrest of the radial growth of the pathogen occurred at a 10% concentration of leaf extract from Adenocallyma alliaceum. A leaf extract of Citrus medica, a root extract of Asparagus adscendens, rhizome extracts of Curcuma longa and Zingiber officinale, and a bulb extract of Allium sativum inhibited up to 100% growth at higher concentrations. A. alliaceum controlled the disease up to 100% by amending its 4% powder in unsterilized soil and 2% in sterilized soil. The population of F. udum was found to be markedly reduced following treatments with plant powders.

  15. Vermicompost and farmyard manure improves food quality, antioxidant and antibacterial potential of Cajanus cajan (L. Mill sp.) leaves.

    Science.gov (United States)

    Das, Subhasish; Hussain, Nazneen; Gogoi, Bhaskarjyoti; Buragohain, Alak Kumar; Bhattacharya, Satya Sundar

    2017-02-01

    Pigeon pea (Cajanus cajan) leaves are a good source of nutrition and health benefitting phenolic compounds. However, its importance has not yet been effectively addressed. Recently, a 2-year field experiment was attempted in an alluvial soil to understand the role of various organic and inorganic fertilisers and their combinations not only on soil quality, but also on production of foremost phenolic compounds and imparting antioxidant and antibacterial properties in C. cajan under vermicompost treatments. Notable enhancements in crude protein, soluble carbohydrate, ash content and total flavonoid content were recorded in Cajanus leaves under vermicompost treatments. We detected a significant rise in carlinoside content in C. cajan leaves, which is known to reduce bilirubin concentration in hepatitis affected human blood. Farmyard manure treatments resulted in a high crude fibre content coupled with a substantially high concentration of total phenols, and chlorophyll. In addition, incorporation of vermicompost with or without inorganic fertiliser in the soil had a significant impact on antioxidant and antibacterial properties of C. cajan leaves. Above and beyond, farmyard manure and vermicompost positively influenced the physico-chemical health of the soil. The present nutrient management scheme based on organic input not only induced a higher yield of C. cajan endowed with improved antioxidant and antibacterial properties, but also enhanced the production of various phenolic compounds. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  16. Morpho-anatomical and growth alterations induced by arsenic in Cajanus cajan (L.) DC (Fabaceae).

    Science.gov (United States)

    Pita-Barbosa, Alice; Gonçalves, Elton Carvalho; Azevedo, Aristéa Alves

    2015-08-01

    Arsenic (As) is a toxic element to most organisms. Studies investigating anatomic alterations due to As exposure in plants are scarce but of utmost importance to the establishment of environmental biomonitoring techniques. So, this study aimed to investigate the effects of As on the development and initial root growth in Cajanus cajan (Fabaceae), characterize and quantify the possible damages, evaluate genotoxic effects, and identify structural markers to be used in environmental bioindication. Plants were exposed hydroponically to 0.5, 1.0, 1.5, and 2.0 mg As L(-1), as sodium arsenate. Growth parameters were measured, and in the end of the exposure, root samples were analyzed for qualitative and quantitative anatomical alterations. Arsenic genotoxicity was evaluated through analysis of the mitotic index in the root apex. Compared to the control, As-treated seedlings showed an altered architecture, with significantly decreased root length (due to the lower mitotic index in the apical meristem and reduced elongation of parenchyma cells) with darkened color, and abnormal development of the root cap. A significant increase in vascular cylinder/root diameter ratio was also detected, due to the reduction of the cellular spaces in the cortex. The secondary xylem vessel elements were reduced in diameter and had sinuous walls. The severest damage was visible in the ramification zone, where uncommon division planes of phellogen and cambium cells and disintegration of the parenchyma cells adjacent to lateral roots were observed. The high sensibility of C. cajan to As was confirmed, since it caused severe damages in root growth and anatomy. The main structural markers for As toxicity were the altered root architecture, with the reduction of the elongation zone and increase of ramification zone length, and the root primordia retained within the cortex. Our results show a new approach about As toxicity and indicate that C. cajan is a promising species to be used for

  17. COMPARATIVE STUDIES ON ANTHELMINTIC ACTIVITY OF LEAF EXTRACT OF MUSA ACUMINATE COLLA AND CAJANUS CAJAN (LINN. LEAF EXTRACT

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    Bhakta Tejendra

    2013-01-01

    Full Text Available Methanolic extract of leaf of Musa acuminate colla and seed of Cajanus cajan (Linn. were taken for the examination of anthelmintic activity on Indian earthworm (Pheretima posthuma. Various concentrations (10mg/ml, 20mg/ml, 30 mg/ml of both plant extract were tested and results were expressed in terms of time of paralysis and time of death of worms. Albendazole of same concentration as like as plant extracts was taken as reference standard and normal saline solution as control. Paralysis time can be determined by vigorous shaking when no movement was observed. When whitish substances were secreted from the body that one was termed as death time. Both the extracts show significant anthelmintic activity but among these two Musa acuminate colla showed more anthelmintic activity than Cajanus cajan (Linn.. Future scope involves isolation of active constituents responsible for this action.

  18. Comparative analysis of genome-wide Mlo gene family in Cajanus cajan and Phaseolus vulgaris.

    Science.gov (United States)

    Deshmukh, Reena; Singh, V K; Singh, B D

    2016-04-01

    The Mlo gene was discovered in barley because the mutant 'mlo' allele conferred broad-spectrum, non-race-specific resistance to powdery mildew caused by Blumeria graminis f. sp. hordei. The Mlo genes also play important roles in growth and development of plants, and in responses to biotic and abiotic stresses. The Mlo gene family has been characterized in several crop species, but only a single legume species, soybean (Glycine max L.), has been investigated so far. The present report describes in silico identification of 18 CcMlo and 20 PvMlo genes in the important legume crops Cajanus cajan (L.) Millsp. and Phaseolus vulgaris L., respectively. In silico analysis of gene organization, protein properties and conserved domains revealed that the C. cajan and P. vulgaris Mlo gene paralogs are more divergent from each other than from their orthologous pairs. The comparative phylogenetic analysis classified CcMlo and PvMlo genes into three major clades. A comparative analysis of CcMlo and PvMlo proteins with the G. max Mlo proteins indicated close association of one CcMlo, one PvMlo with two GmMlo genes, indicating that there was no further expansion of the Mlo gene family after the separation of these species. Thus, most of the diploid species of eudicots might be expected to contain 15-20 Mlo genes. The genes CcMlo12 and 14, and PvMlo11 and 12 are predicted to participate in powdery mildew resistance. If this prediction were verified, these genes could be targeted by TILLING or CRISPR to isolate powdery mildew resistant mutants.

  19. Characterization of five fungal endophytes producing Cajaninstilbene acid isolated from pigeon pea [Cajanus cajan (L. Millsp].

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    Yuan Gao

    Full Text Available Five fungal endophytes (K4, K5, K6, K9, K14 producing Cajaninstilbene acid (CSA, 3-hydroxy-4-prenyl-5-methoxystilbene-2-carboxylic acid were isolated from the roots of pigeon pea [Cajanus cajan (L. Millsp.]. CSA is responsible for the prominent pharmacological activities in pigeon pea. The amount of CSA in culture solution varied among the five fungal endophytes. K4 produced the highest levels of CSA (1037.13 µg/L among the endophytes tested after incubation for five days. Both morphological characteristics and molecular methods were used for species identification of fungal endophytes. The five endophytic isolates were characterized by analyzing the internal transcribed spacer (ITS rRNA and β-tubulin genes. The K4, K5, K9 and K14 strains isolated from pigeon pea roots were found to be closely related to the species Fusarium oxysporum. K6 was identified as Neonectria macrodidym. The present study is the first report on the isolation and identification of fungal endophytes producing CSA in pigeon pea. The study also provides a scientific base for large scale production of CSA.

  20. EL GUANDUL (Cajanus cajan UNA ALTERNATIVA EN LA INDUSTRIA DE LOS ALIMENTOS

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    CARMEN LUCIA NAVARRO V

    Full Text Available Debido a la situación actual de nutrición inadecuada en la población de muchos países, entre ellos Colombia, es necesario ofrecer alternativas de fuentes ricas en proteínas y de bajo costo. El guandul (Cajanus cajan es una importante leguminosa que contiene una moderada cantidad de proteínas, calorías, ciertos minerales y vitaminas, su uso en alimentos es limitada por la presencia de factores antinutricionales, que pueden ser disminuidos o eliminados mediante la implementación de tratamientos. Las proteínas del guandul presentan buena calidad y propiedades funcionales adecuadas para la industria de alimentos que pueden ser aprovechas en productos cárnicos, lácteos y panadería. El propósito de esta revisión es presentar una visión general de las habilidades nutricionales, propiedades funcionales yoportunidades de aplicación del guandul en diversas aplicaciones en la industria de alimentos.

  1. Characterization of five fungal endophytes producing Cajaninstilbene acid isolated from pigeon pea [Cajanus cajan (L.) Millsp].

    Science.gov (United States)

    Gao, Yuan; Zhao, Jin Tong; Zu, Yuan Gang; Fu, Yu Jie; Wang, Wei; Luo, Meng; Efferth, Thomas

    2011-01-01

    Five fungal endophytes (K4, K5, K6, K9, K14) producing Cajaninstilbene acid (CSA, 3-hydroxy-4-prenyl-5-methoxystilbene-2-carboxylic acid) were isolated from the roots of pigeon pea [Cajanus cajan (L.) Millsp.]. CSA is responsible for the prominent pharmacological activities in pigeon pea. The amount of CSA in culture solution varied among the five fungal endophytes. K4 produced the highest levels of CSA (1037.13 µg/L) among the endophytes tested after incubation for five days. Both morphological characteristics and molecular methods were used for species identification of fungal endophytes. The five endophytic isolates were characterized by analyzing the internal transcribed spacer (ITS) rRNA and β-tubulin genes. The K4, K5, K9 and K14 strains isolated from pigeon pea roots were found to be closely related to the species Fusarium oxysporum. K6 was identified as Neonectria macrodidym. The present study is the first report on the isolation and identification of fungal endophytes producing CSA in pigeon pea. The study also provides a scientific base for large scale production of CSA.

  2. Potencial de Cajanus cajan e Crotalaria spectabilis parafitorremediação: absorção de arsênio e respostas antioxidativas Potential of Cajanus cajan and Crotalaria spectabilis for phytoremediation: arsenic absorption and antioxidative responses

    Directory of Open Access Journals (Sweden)

    Rafaella Teles Arantes Felipe

    2009-04-01

    Full Text Available Neste estudo, avaliaram-se os efeitos tóxicos do arsênio (As sobre o crescimento e atividade de enzimas antioxidativas dismutase do superóxido (SOD, catalase (CAT, peroxidases (POX, peroxidase do ascorbato peroxidase (APX e glutationa redutase (GR em plantas de Cajanus cajan e Crotalaria spectabili. Plantas das duas espécies foram expostas a diferentes concentrações de As, em solução nutritiva de Hoagland, pH 6,5. A taxa de crescimento relativo (TCR das duas espécies reduziu-se com o aumento da concentração de As na solução nutritiva, sendo de forma mais intensa em C. cajan. A concentração de As na solução capaz de reduzir em 50% a TCR de C. cajan foi de 0,93 mg L-1, enquanto em C. spectabilis foi de 4,80 mg L-1. As duas espécies apresentaram reduzida translocação de As para a parte aérea, sendo em raízes de C. spectabilis observada a maior concentração desse elemento. C. cajan exposto ao As teve as atividades das enzimas CAT, POX e APX reduzidas, ao contrário do que ocorreu em C. spectabilis. Esta espécie, quando submetida ao As, apresentou incremento na atividade de todas as enzimas avaliadas. A maior tolerância ao As observada em C. spectabilis pode estar relacionada à maior capacidade em reter o As nas raízes e à indução das enzimas SOD, CAT, POX, APX e GR. Dessa forma, C. spectabilis é uma espécie com potencial para utilização em programas de revegetação de áreas contaminadas com As.The toxic effects of Arsenic (As on the growth and activities of the antioxidative enzymes superoxide dismutase (SOD, catalase (CAT, peroxidase (POX, ascorbate peroxidase (APX and glutathione reductase (GR of Cajanus cajan and Crotalaria spectabilsi were studied. AQUI Plants of the two species were exposed to different As concentrations in Hoagland's nutrient solution, pH 6.5. The relative growth rate (RGR of the two species reduced with the increase in As concentration in the nutrient solution, especially in C. cajan

  3. Morphological characterization and assessment of genetic diversity in minicore collection of pigeonpea [Cajanus Cajan (L. Millsp.

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    Muniswamy, S., Lokesha, R. *, Dharmaraj, P.S., Yamanura1 and Diwan, J.R.

    2014-06-01

    Full Text Available An investigation was undertaken to ascertain the extent of genetic diversity present among 196 pigeon pea genotypes using D2 statistic. A wider genetic diversity was observed for nine characters as evidenced by formation of 13 clusters. Number of pods per plant contributed most (59.83% towards divergence, followed by plant height ( 21.55 The highest inter cluster distance was observed between the cluster XIII and VII, followed by cluster V and XIII, II and XIII and cluster XII and VII, which indicates that the crosses among the genotypes between these clusters may result in better segregants and high heterotic combinations. Cluster mean analysis indicated that cluster V contains dwarf and early maturing genotypes and cluster XIII possess high yielding entries. Morphological characterization was also carried out for 15 traits can be used in varietal purification and seed production.

  4. AGROBACTERIUM MEDIATED TRANSFORMATION OF PIGEONPEA (CAJANUS CAJAN L MILLLSP VAR LRG-41 FROM AXILLARY BUD

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    T. Raghavendra

    2014-02-01

    Full Text Available A reliable method of plant regeneration has been achieved from Axillary buds. Shoots appeared from explants when cultured on Murashige and skoog (MS medium supplemented with BAP (Benzyl amino purine, Napthalene acetic acid (NAA and Kinetin at various combinations. Elongated shoots were rooted with 70.6% rooting frequency in MS medium with indole buteric acid (IBA at 1.0mg/l. The rooted plantlets were established well in soilrite mixture medium with 91% success and days taken for acclimatization were 12.8 days. This protocol was used in Agrobacterium mediated transformation. The transformation was carried out using the Agrobacterium strain LBA4404 containing the binary vector pCAMBIA2301 harboring npt II as selectable marker and GUS as reporter gene.

  5. AGROBACTERIUM MEDIATED TRANSFORMATION OF PIGEONPEA (CAJANUS CAJAN L MILLLSP VAR LRG-41 FROM AXILLARY BUD

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    T. Raghavendra

    2014-03-01

    Full Text Available A reliable method of plant regeneration has been achieved from Axillary buds. Shoots appeared from explants when cultured on Murashige and skoog (MS medium supplemented with BAP (Benzyl amino purine, Napthalene acetic acid (NAA and Kinetin at various combinations. Elongated shoots were rooted with 70.6% rooting frequency in MS medium with indole buteric acid (IBA at 1.0mg/l. The rooted plantlets were established well in soilrite mixture medium with 91% success and days taken for acclimatization were 12.8 days. This protocol was used in Agrobacterium mediated transformation. The transformation was carried out using the Agrobacterium strain LBA4404 containing the binary vector pCAMBIA2301 harboring npt II as selectable marker and GUS as reporter gene.

  6. Effect of Some Stresses on Free Proline Content During Pigeonpea (Cajanas cajan Seed Germination

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    Bhamburdekar S.B.

    2011-09-01

    Full Text Available Plants accumulate proline under a variety of stress conditions thereby preventing stress-caused damages. The proline accumulation in germinating seeds of pigeonpea in response to NaCl, Boron and Aluminium treatments were studied. Results showed increased accumulation of proline in salinity stressed germinating seeds. As compare to 100 ppm boron, 10 and 50 ppm of boron treatments promoted proline levels at different stages of seed germination. The content of proline was found to be induced by all aluminium concentrations particularly at 48, 72 and 120 hrs. of seed germination. Whereas reverse pattern of proline accumulation was observed at 96 hrs stage under all studied stresses.

  7. Ethnobotanical survey and cytotoxicity testing of plants of South-western Nigeria used to treat cancer, with isolation of cytotoxic constituents from Cajanus cajan Millsp. leaves.

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    Ashidi, J S; Houghton, P J; Hylands, P J; Efferth, T

    2010-03-24

    There is only scant literature on the anticancer components of medicinal plants from Nigeria, yet traditional healers in the area under study claim to have been managing the disease in their patients with some success using the species studied. To document plants commonly used to treat cancer in South-western Nigeria and to test the scientific basis of the claims using in vitro cytotoxicity tests. Structured questionnaires were used to explore the ethnobotanical practices amongst the traditional healers. Methanol extracts of the most common species cited were screened for cytotoxicity using the sulforhodamine B (SRB) assay in both exposure and recovery experiments. Three cancer cell lines (human breast adenocarcinoma cell line MCF-7, human large cell lung carcinoma cell line COR-L23 and human amelanotic melanoma C32) and one normal cell line (normal human keratinocytes SVK-14) were used for the screening of the extracts and the fractions obtained. The extract of Cajanus cajan showed considerable activity and was further partitioned and the dichloromethane fraction was subjected to preparative chomatography to yield six compounds: hexadecanoic acid methyl ester, alpha-amyrin, beta-sitosterol, pinostrobin, longistylin A and longistylin C. Pinostrobin and longistylins A and C were tested for cytotoxicity on the cancer cell lines. In addition, an adriamycin-sensitive acute T-lymphoblastic leukaemia cell line (CCRF-CEM) and its multidrug-resistant sub-line (CEM/ADR5000) were used in an XTT assay to evaluate the activity of the pure compounds obtained. A total of 30 healers from S W Nigeria were involved in the study. 45 species were recorded with their local names with parts used in the traditional therapeutic preparations. Cytotoxicity (IC(50) values less than 50 microg/mL) was observed in 5 species (Acanthospermum hispidum, Cajanus cajan, Morinda lucida, Nymphaea lotus and Pycnanthus angolensis). Acanthospermum hispidum and Cajanus cajan were the most active. The

  8. Constituents of Cajanus cajan (L.) Millsp., Moringa oleifera Lam., Heliotropium indicum L. and Bidens pilosa L. from Nigeria.

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    Ogunbinu, Akinola O; Flamini, Guido; Cioni, Pier L; Adebayo, Muritala A; Ogunwande, Isiaka A

    2009-04-01

    The essential oils of four plant species from Nigeria have been extracted by hydrodistillation and analyzed by GC and GC-MS. The oils of Cajanus cajan were comprised of sesquiterpenes (92.5%, 81.2% and 94.3% respectively in the leaves, stem and seeds). The major compounds identified were alpha-himachalene (9.0-11.5%), beta-himachalene (8.0-11.0%), gamma-himachalene (6.9-8.1%), alpha-humulene (7.1-8.7%) and alpha-copaene (4.5-5.6%). However, monoterpenoid compounds (81.8%) dominated the oil of Moringa oleifera with an abundance of alpha-phellandrene (25.2%) and p-cymene (24.9%). On the other hand, aldehydes (52.8%) occurred in the highest amount in Heliotropium indicum, represented by phenylacetaldehyde (22.2%), (E)-2-nonenal (8.3%) and (E, Z)-2-nonadienal (6.1%), with a significant quantity of hexahydrofarnesylacetone (8.4%). The leaf and stem oils of Bidens pilosa were dominated by sesquiterpenes (82.3% and 59.3%, respectively). The main compounds in the leaf oil were caryophyllene oxide (37.0%), beta-caryophyllene (10.5%) and humulene oxide (6.0%), while the stem oils had an abundance of hexahydrofarnesyl acetone (13.4%), delta-cadinene (12.0%) and caryophyllene oxide (11.0%). The observed chemical patterns differ considerably from previous investigations.

  9. Application of fly ash on the growth performance and translocation of toxic heavy metals within Cajanus cajan L.: implication for safe utilization of fly ash for agricultural production.

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    Pandey, Vimal Chandra; Abhilash, P C; Upadhyay, Raj Narayan; Tewari, D D

    2009-07-15

    The present study was undertaken to examine the influence of the application of fly ash (FA) into garden soil for Cajanus cajan L. cultivation and on accumulation and translocation of hazardous metals from FA to edible part. Numerous studies have been reported on the growth and yield of agricultural crops under FA stress; however, there is a dearth of studies recommending the safe utilization of fly ash for crop production. Pot experiments were conducted on C. cajan L., a widely cultivating legume in India for its highly nutritious seeds. C. cajan L. were grown in garden soil and amended with varying concentrations of FA in a weight/weight ratio (0%, 25%, 50% and 100%; w/w). Incorporation of fly ash from 25% to 100% in garden soil increases the levels of pH, particle density, porosity and water holding capacity from 3.47% to 26.39%, 3.98% to 26.14%, 37.50% to 147.92% and 163.16% to 318.42%, respectively, than the control while bulk density decrease respectively from 8.94% to 48.89%. Pot experiment found that accumulation and translocation of heavy metals in tested plant depends on the concentration of FA. Addition of FA at lower concentration (25%) had shown positive results in most of the studied parameters of growth and yield (14.23% than control). The experimental results confirmed that lower concentration of FA (25%) is safe for C. cajan cultivation, which not only enhanced the yield of C. cajan L. significantly but also ensured the translocation of heavy metals to edible parts within the critical limits.

  10. Cytoplasmic male sterility-associated chimeric open reading frames identified by mitochondrial genome sequencing of four Cajanus genotypes.

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    Tuteja, Reetu; Saxena, Rachit K; Davila, Jaime; Shah, Trushar; Chen, Wenbin; Xiao, Yong-Li; Fan, Guangyi; Saxena, K B; Alverson, Andrew J; Spillane, Charles; Town, Christopher; Varshney, Rajeev K

    2013-10-01

    The hybrid pigeonpea (Cajanus cajan) breeding technology based on cytoplasmic male sterility (CMS) is currently unique among legumes and displays major potential for yield increase. CMS is defined as a condition in which a plant is unable to produce functional pollen grains. The novel chimeric open reading frames (ORFs) produced as a results of mitochondrial genome rearrangements are considered to be the main cause of CMS. To identify these CMS-related ORFs in pigeonpea, we sequenced the mitochondrial genomes of three C. cajan lines (the male-sterile line ICPA 2039, the maintainer line ICPB 2039, and the hybrid line ICPH 2433) and of the wild relative (Cajanus cajanifolius ICPW 29). A single, circular-mapping molecule of length 545.7 kb was assembled and annotated for the ICPA 2039 line. Sequence annotation predicted 51 genes, including 34 protein-coding and 17 RNA genes. Comparison of the mitochondrial genomes from different Cajanus genotypes identified 31 ORFs, which differ between lines within which CMS is present or absent. Among these chimeric ORFs, 13 were identified by comparison of the related male-sterile and maintainer lines. These ORFs display features that are known to trigger CMS in other plant species and to represent the most promising candidates for CMS-related mitochondrial rearrangements in pigeonpea.

  11. A Halogen-Containing Stilbene Derivative from the Leaves of Cajanus cajan that Induces Osteogenic Differentiation of Human Mesenchymal Stem Cells

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    Jia-Zhong Cai

    2015-06-01

    Full Text Available A new natural halogen-containing stilbene derivative was isolated from the leaves of Cajanus cajan (L. Millsp. and identified as 3-O-(3-chloro-2-hydroxyl-propanyl-longistylin A by comprehensive spectroscopic and chemical analysis, and named cajanstilbene H (1. It is the first halogen-containing stilbene derivative found from plants. In human mesenchymal stem cells (hMSC from bone marrow, 1 did not promote cell proliferation, but distinctly enhanced osteogenic differentiation of hMSC in time- and dose-dependent manners. In six human cancer cell lines, 1 showed a moderate inhibitory effect on cell proliferation, with IC50 values of 21.42–25.85 μmol·L−1.

  12. Purification and Partial Characterization of Trypsin-Specific Proteinase Inhibitors from Pigeonpea Wild Relative Cajanus platycarpus L.(Fabaceae active against Gut Proteases of Lepidopteran pest Helicoverpa armigera

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    Marri Swathi

    2016-09-01

    Full Text Available AbstractProteinase inhibitors (PIs are natural defense proteins of plants found to be active against gut proteases of various insects. A pigeonpea wild relative Cajanus platycarpus was identified as a source of resistance against Helicoverpa armigera, a most devastating pest of several crops including pigeonpea. In the light of earlier studies, trypsin-specific PIs (CpPI 63 were purified from mature dry seeds of C. platycarpus (ICPW-63 and characterized their biochemical properties in contributing to H. armigera resistance. CpPI 63 possessed significant H. armigera gut trypsin-like proteinase inhibitor (HGPI activity than trypsin inhibitor (TI activity. Analysis of CpPI 63 using two-dimensional (2-D electrophoresis and matrix assisted laser desorption ionization time-of-flight (MALDI-TOF mass spectrometry revealed that it contained several isoinhibitors and small oligomers with masses ranging between 6-58 kDa. The gelatin activity staining studies suggest that these isoinhibitors and oligomers possessed strong inhibitory activity against H. armigera gut trypsin-like proteases (HGPs. The N-terminal sequence of the isoinhibitors (pI 6.6 and pI 5.6 of CpPI 63 exhibited 80% homology with several Kunitz trypsin inhibitors (KTIs as well as miraculin-like proteins (MLPs. Further, modification of lysine residue(s lead to 80% loss in both TI and HGPI activities of CpPI 63. In contrast, the TI and HGPI activities of CpPI 63 were stable over a wide range of temperature and pH conditions. The reported results provide a biochemical basis for pod borer resistance in C. platycarpus.

  13. Evaluación de tres niveles de reemplazo del concentrado comercial por guandul (Cajanus cajan L. en conejos de ceba

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    Borrero Giovanny

    1990-06-01

    Full Text Available En Palmira Colombia se realizó un ensayo utilizando heno de guandul, Cajanus cajan L., para reemplazar concentrado comercial en niveles de 25,50,75 y 100 % con un tratamiento testigo de 100% concentrado comercial. Se emplearon 30 gazapos Nueva Zelanda blancos con una edad de 35 días, distribuídos en un diseño de bloques completamente al azar (BOCA donde el peso fue el factor de bloqueo. Cada bloque de 10 animales. Cada unidad experimental estaba constituida por dos animales alojados en jaulas de 0.3 x 0.6. Cada tratamiento tuvo 3 repeticiones. Se evaluaron las variables ganancia de peso, consumo de materia seca, conversión alimenticia, rendimiento en canal y semanas para alcanzar 2 kg. A medida que aumentó el nivel de guandul disminuyó la ganancia de peso/animal/día y el rendimiento en canal y se necesitó mayor número de semanas para alcanzar los 2 kg de peso. Se registraron mejores resultados en el testigo que en los tratamientos con guandul, sin embargo los resultados del tratamiento del 75 % de concentrado + 25 % de guandul, fueron similares a los del testigo y fue el tratamiento de mayor beneficio neto.In Palmira, Colombia was done an experiment using guandul (Cajanus cajan hay for replacing commercial feed in 25, 50, 75 and 100 % levels with a control treatment, 100 % commercial feed. Thirty white New Zeland rabbits 35 days old , were used and distributed in a Randomized Block Design (RDD where weight was the blocking factor. Each block had 10 animals. Every experimental unit was composed for two rabbits located in one e age (0.3 m x 0.6 m. Each treatment had 3 replications. The following variables were evaluated: weight gain, dry matter consumption, feed efficiency, carcass percentage ad weeks to reach the 2 kg weight. For every increase in guandul hay levels, there was a reduction in daily weight gain and carcass percentage and it was required more time to reach the 2 kg weight. When the control treatment was compared with the

  14. Effect of Agricultural Amendments on Cajanus cajan (Pigeon Pea and Its Rhizospheric Microbial Communities--A Comparison between Chemical Fertilizers and Bioinoculants.

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    Rashi Gupta

    Full Text Available Inoculation of leguminous seeds with bioinoculants has been practiced in agriculture for decades to ameliorate grain yield by enhanced growth parameters and soil fertility. However, effective enhancement of plant growth parameters results not only from the direct effects these bioinoculants impose on them but also from their non-target effects. The ability of bioinoculants to reduce the application of chemicals for obtaining optimum yield of legume appears to be of great ecological and economic importance. In the present study, we compared the influence of seed inoculation of Cajanus cajan with a microbial consortium, comprising Bacillus megaterium, Pseudomonas fluorescens and Trichoderma harzianum, with that of application of chemical fertilizers on plant's growth parameters and its rhizospheric microbial communities. Real-time PCR assay was carried out to target the structure (16S rRNA and function (nitrogen cycle of rhizospheric microbiota, using both DNA and RNA as markers. The results showed that the microbial consortium was the most efficient in increasing grain yield (2.5-fold, even better than the recommended dose of chemical fertilizers (by 1.2-fold and showed enhancement in nifH and amoA transcripts by 2.7- and 2.0-fold, respectively. No adverse effects of bioinoculants' application were observed over the rhizospheric microbial community, rendering the consortium to be safe for release in agricultural fields.

  15. Effect of Agricultural Amendments on Cajanus cajan (Pigeon Pea) and Its Rhizospheric Microbial Communities--A Comparison between Chemical Fertilizers and Bioinoculants.

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    Gupta, Rashi; Bisaria, V S; Sharma, Shilpi

    2015-01-01

    Inoculation of leguminous seeds with bioinoculants has been practiced in agriculture for decades to ameliorate grain yield by enhanced growth parameters and soil fertility. However, effective enhancement of plant growth parameters results not only from the direct effects these bioinoculants impose on them but also from their non-target effects. The ability of bioinoculants to reduce the application of chemicals for obtaining optimum yield of legume appears to be of great ecological and economic importance. In the present study, we compared the influence of seed inoculation of Cajanus cajan with a microbial consortium, comprising Bacillus megaterium, Pseudomonas fluorescens and Trichoderma harzianum, with that of application of chemical fertilizers on plant's growth parameters and its rhizospheric microbial communities. Real-time PCR assay was carried out to target the structure (16S rRNA) and function (nitrogen cycle) of rhizospheric microbiota, using both DNA and RNA as markers. The results showed that the microbial consortium was the most efficient in increasing grain yield (2.5-fold), even better than the recommended dose of chemical fertilizers (by 1.2-fold) and showed enhancement in nifH and amoA transcripts by 2.7- and 2.0-fold, respectively. No adverse effects of bioinoculants' application were observed over the rhizospheric microbial community, rendering the consortium to be safe for release in agricultural fields.

  16. Use of Lactobacillus acidophilus and Lactobacillus casei for a potential probiotic legume-based fermented product using pigeon pea (Cajanus cajan).

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    Parra, K; Ferrer, M; Piñero, M; Barboza, Y; Medina, L M

    2013-02-01

    The aim of the present study was to evaluate the use of pigeon pea (Cajanus cajan) as an appropriate substrate in the production of a legume-based fermented product with Lactobacillus acidophilus ATCC 314 or Lactobacillus casei ATCC 393 and then to ascertain the effects of the addition of ingredients such as powdered milk and banana or strawberry sauce. The products were analyzed for viable cell counts, pH, and sensory attributes during product manufacture and throughout the refrigerated storage period at 3, 7, 14, 21, and 28 days. Nine types of products were produced. At the end of the storage period, the viability of L. acidophilus was above 7 log CFU/g in the presence of milk and 20% sucrose fruit sauce. For products with L. casei, the lack of ingredients such as milk caused no significant loss in viability; however, a high concentration of sucrose in the fruit sauce was an important factor in maintaining a high L. casei population. L. casei had high viability and good sensory attributes. Both strains could be considered suitable for a pigeon pea-based fermented potential probiotic product and a low-cost protein source.

  17. Comparative study of antidiabetic activity of Cajanus cajan and Tamarindus indica in alloxan-induced diabetic mice with a reference to in vitro antioxidant activity

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    Laizuman Nahar

    2014-01-01

    Full Text Available Background: Oxidative stress not only develops complications in diabetic (type 1 and type 2 but also contributes to beta cell destruction in type 2 diabetes in insulin resistance hyperglycemia. Glucose control plays an important role in the pro-oxidant/antioxidant balance. Some antidiabetic agents may by themselves have antioxidant properties independently of their role on glucose control. Objective: The present investigation draws a comparison of the protective antioxidant activity, total phenol content and the antihyperglycemic activity of the methanolic extract of Cajanus cajan root (MCC and Tamarindus indica seeds (MTI. Materials and Methods: Antidiabetic potentials of the plant extracts were evaluated in alloxan-induced diabetic Swiss albino mice. The plant extracts at the doses of 200 and 400 mg/kg body weight was orally administered for glucose tolerance test during 1-hour study and hypoglycemic effect during 5-day study period in comparison with reference drug Metformin HCl (50 mg/kg. In vitro antioxidant potential of MCC and MTI was investigated by using 1, 1- diphenyl-2-picrylhydrazyl (DPPH free radical scavenging activity at 517 nm. Total phenolic content, total antioxidant capacity and reducing power activity was also assayed. Results: There was a significant decrease in fasting serum glucose level (P < 0.001, reduction in blood glucose level (P < 0.001 in 5-days study, observed in the alloxan-induced diabetic mice. The reduction efficacy of blood glucose level of both the extracts is proportional to their dose but MCC is more potent than MTI. Antioxidant study and quantification of phenolic compound of both the extracts revealed that they have high antioxidant capacity. Conclusion: These studies showed that MCC and MTI have both hypoglycemic and antioxidant potential but MCC is more potent than MTI. The present study suggests that both MCC and MTI could be used in managing oxidative stress.

  18. Environmental influences on pigeonpea-Fusarium udum interactions and stability of genotypes to Fusarium wilt.

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    Mamta eSharma

    2016-03-01

    Full Text Available Fusarium wilt (Fusarium udum Butler is an important biotic constraint to pigeonpea (Cajanus cajan L. production worldwide. Breeding for fusarium wilt resistance continues to be an integral part of genetic improvement of pigeonpea. Therefore, the study was aimed to identify and validate resistant genotypes to fusarium wilt and determine the magnitude of genotype × environment (G × E interactions through multi-environment and multi-year screening. Total 976 genotypes including germplasm and breeding lines were screened against wilt in wilt sick plot at Patancheru, India. Ninety two genotypes found resistant to wilt were tested further for two more years in wilt sick plot at Patancheru and Pigeonpea Wilt Nursery (PWN comprising of 29 genotypes was constituted. PWN was evaluated at nine locations representing different agro-climatic zones of India for wilt resistance during two crop seasons 2007/08 and 2008/09. Genotypes (G, environment (E and G × E interactions biplot partitioned main effect into G, E and G × E interactions and significant effects (p≤0.001 were obtained for wilt incidence. Genotype contributed 36.51% of resistance variation followed by environment (29.32%. GGE biplot in integration with boxplot and multiple comparison tests enabled us to identify seven stable genotypes (ICPL 20109, ICPL 20096, ICPL 20115, ICPL 20116, ICPL 20102, ICPL 20106 and ICPL 20094 based on their performance across diverse environments. These genotypes have broad based resistance and can be exploited in pigeonpea breeding program.

  19. Genetic diversity analysis among pigeonpea genotypes adapted to South American regions based on microsatellite markers

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    Adna Cristina Barbosa de Sousa

    2011-08-01

    Full Text Available The pigeonpea [Cajanus cajan (L Millspaugh] is one of the most important perennial legume crops utilized in the food, fodder, soil conservation, crop-livestock integrated systems, reclaiming of degraded pastures and symbiotic nitrogen fixation. Microsatellite markers were used to estimate the genetic diversity of 77 pigeonpea genotypes selected from the germplasm collections at Embrapa Cattle-Southeast and, to evaluate their transferability to Phaseolus vulgaris and Vigna unguiculata species. The number of alleles per locus ranged from 2 to12, with an average of 5.1 alleles. The PIC values ranged from 0.11 to 0.80 (average 0.49 and the D values from 0.23 to 0.91 (average 0.58. The averages of observed and expected heterozygosity were 0.25 and 0.47, respectively, showing a deficit in heterozygosity. A model-based Bayesian approach implemented in the software STRUCTURE was used to assign genotypes into clusters. A dendrogram was constructed based on the modified Roger's genetic distances using a neighbor-joining method (NJ. A total of four clusters were assembled by STRUCTURE and a strong tendency of correspondence between the Bayesian clusters in the NJ tree was observed. The genetic distance ranged from 0.09 to 0.62 (average 0.37, showing a low genetic diversity in the pigeonpea genotypes. Transferability of pigeonpea-specific microsatellites revealed a cross-amplification and the presence of polymorphic alleles in P. vulgaris and V. unguiculata.

  20. Assessment of beneficial role of an insectivorous bird, jungle babbler (Turdoides striatus predation, on Helicoverpa armigera infesting pigeon pea (Cajanus cajan crop

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    Bharucha Bhavna

    2010-06-01

    Full Text Available Jungle babbler (Turdoides striatus, a widely spread sub-tropical insectivorous passerine is considered beneficial to agro-ecosystem, as they devour voraciously on insect matter especially Helicoverpa armigera, the gram pod borer, a notorious pest infesting and causing heavy loses to crops like pigeon pea (Cajanus cajan which is a vital crop of semi-arid tropical and subtropical farming system, providing high quality vegetable protein. Helicoverpa is known to feed on flowers, pods, and seeds and is the most important biotic constraint affecting pigeon pea yields. Jungle babblers have a peculiar foraging style which helps expose the Helicoverpa larvae as well as pupae through various phenological stages of pigeon pea. For comparative assessment of their beneficial role and as a possible bio control agent, in Baroda city (State of Gaujarat, India, was studied, two crops of pigeon pea (insecticide treated and untreated (control were selected. In both treated and control crops, the number of jungle babblers were maximum in pigeon pea fields during october and november in both small pod stage and large pod stage which had heavy infestation of Helicoverpa. Least number of birds was seen during the flowering stage in September. Later in treatment crop three applications of Dunnate and Monocrotophos insecticide spray was done after which the pest population decreased which is reflected in number of birds in the field, while the bird number in control crops grew since insecticide spray was not done and number of larvae increased with the stage of the crop. Along with the main crop pigeon pea, comparative study was also done to see the food preference by these birds in crops like sorghum, maize, cow pea and ploughed and unploughed fields. Maximum number of birds was seen in unploughed field and least in sorghum suggesting that Helicoverpa is preferred food over sorghum grains thus pigeon pea

  1. Assessment of beneficial role of an insectivorous bird, jungle babbler (Turdoides striatus predation, on Helicoverpa armigera infesting pigeon pea (Cajanus cajan crop

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    Bhavna Bharucha

    2010-04-01

    Full Text Available Jungle babbler (Turdoides striatus, a widely spread sub-tropical insectivorous passerine is considered beneficial to agro-ecosystem, as they devour voraciously on insect matter especially Helicoverpa armigera, the gram pod borer, a notorious pest infesting and causing heavy loses to crops like pigeon pea (Cajanus cajan which is a vital crop of semi-arid tropical and subtropical farming system, providing high quality vegetable protein. Helicoverpa is known to feed on flowers, pods, and seeds and is the most important biotic constraint affecting pigeon pea yields. Jungle babblers have a peculiar foraging style which helps expose the Helicoverpa larvae as well as pupae through various phenological stages of pigeon pea. For comparative assessment of their beneficial role and as a possible bio control agent, in Baroda city (State of Gaujarat, India, was studied, two crops of pigeon pea (insecticide treated and untreated (control were selected. In both treated and control crops, the number of jungle babblers were maximum in pigeon pea fields during october and november in both small pod stage and large pod stage which had heavy infestation of Helicoverpa. Least number of birds was seen during the flowering stage in September. Later in treatment crop three applications of Dunnate and Monocrotophos insecticide spray was done after which the pest population decreased which is reflected in number of birds in the field, while the bird number in control crops grew since insecticide spray was not done and number of larvae increased with the stage of the crop. Along with the main crop pigeon pea, comparative study was also done to see the food preference by these birds in crops like sorghum, maize, cow pea and ploughed and unploughed fields. Maximum number of birds was seen in unploughed field and least in sorghum suggesting that Helicoverpa is preferred food over sorghum grains thus pigeon pea and sorghum can be used as mixed crops to protect the crop from

  2. De novo Assembly and Characterization of Cajanus scarabaeoides (L. Thouars Transcriptome by Paired-End Sequencing

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    Deepti Nigam

    2017-07-01

    Full Text Available Pigeonpea [Cajanus cajan (L. Millsp.] is a heat and drought resilient legume crop grown mostly in Asia and Africa. Pigeonpea is affected by various biotic (diseases and insect pests and abiotic stresses (salinity and water logging which limit the yield potential of this crop. However, resistance to all these constraints is not readily available in the cultivated genotypes and some of the wild relatives have been found to withstand these resistances. Thus, the utilization of crop wild relatives (CWR in pigeonpea breeding has been effective in conferring resistance, quality and breeding efficiency traits to this crop. Bud and leaf tissue of Cajanus scarabaeoides, a wild relative of pigeon pea were used for transcriptome profiling. Approximately 30 million clean reads filtered from raw reads by removal of adaptors, ambiguous reads and low-quality reads (3.02 gigabase pairs were generated by Illumina paired-end RNA-seq technology. All of these clean reads were pooled and assembled de novo into 1,17,007 transcripts using the Trinity. Finally, a total of 98,664 unigenes were derived with mean length of 396 bp and N50 values of 1393. The assembly produced significant mapping results (73.68% in BLASTN searches of the Glycine max CDS sequence database (Ensembl. Further, uniprot database of Viridiplantae was used for unigene annotation; 81,799 of 98,664 (82.90% unigenes were finally annotated with gene descriptions or conserved protein domains. Further, a total of 23,475 SSRs were identified in 27,321 unigenes. This data will provide useful information for mining of functionally important genes and SSR markers for pigeonpea improvement.

  3. Assessment of beneficial role of an insectivorous bird, jungle babbler (Turdoides striatus predation, on Helicoverpa armigera infesting pigeon pea (Cajanus cajan crop Evaluación del rol benéfico de Turdoides striatus como predator de Helicoverpa armigera en el cultivo de guandul (Cajanus cajan

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    Bhavna Bharucha

    2010-04-01

    Full Text Available Jungle babbler (Turdoides striatus, a widely spread sub-tropical insectivorous passerine is considered beneficial to agro-ecosystem, as they devour voraciously on insect matter especially Helicoverpa armigera, the gram pod borer, a notorious pest infesting and causing heavy loses to crops like pigeon pea (Cajanus cajan which is a vital crop of semi-arid tropical and subtropical farming system, providing high quality vegetable protein. Helicoverpa is known to feed on flowers, pods, and seeds and is the most important biotic constraint affecting pigeon pea yields. Jungle babblers have a peculiar foraging style which helps expose the Helicoverpa larvae as well as pupae through various phenological stages of pigeon pea. For comparative assessment of their beneficial role and as a possible bio control agent, in Baroda city (State of Gaujarat, India, was studied, two crops of pigeon pea (insecticide treated and untreated (control were selected. In both treated and control crops, the number of jungle babblers were maximum in pigeon pea fields during october and november in both small pod stage and large pod stage which had heavy infestation of Helicoverpa. Least number of birds was seen during the flowering stage in September. Later in treatment crop three applications of Dunnate and Monocrotophos insecticide spray was done after which the pest population decreased which is reflected in number of birds in the field, while the bird number in control crops grew since insecticide spray was not done and number of larvae increased with the stage of the crop. Along with the main crop pigeon pea, comparative study was also done to see the food preference by these birds in crops like sorghum, maize, cow pea and ploughed and unploughed fields. Maximum number of birds was seen in unploughed field and least in sorghum suggesting that Helicoverpa is preferred food over sorghum grains thus pigeon pea and sorghum can be used as mixed crops to protect the crop from

  4. Field Insect Pests and Crop Damage Assessment of Pigeon Pea (Cajanus cajan [L.] Huth grown under Ratoon and in Mixture with Maize Evaluación de Campo de Insectos Pestes y Daño del cultivo de Gandul (Cajanus cajan [L.] Huth creciendo bajo Rebrotes o en Mezcla con Maíz

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    Madang A Dasbak

    2012-03-01

    Full Text Available The widespread adoption of cropping systems that are sustainable and environmentally friendly is vital for the macroeconomic survival of civilization. Intercropping could ensure stability of insect populations in a system. A 3-yr (2005-2007 field trial was therefore carried out in Nigeria under regular and ratoon crops to evaluate five recently developed pigeon pea (Cajanus cajan [L.] Huth genotypes (ICPL 87, ICPL 161, ICPL 85063, ICP 7120, and ICPL 87119 from the International Crops Research Institute for Semi-Arid Tropics (ICRISAT and one local variety for their relative performance and susceptibility to insect pests. The pigeon pea genotypes were in a mixture with two maize genotypes (Zea mays L., open-pollinated and hybrid in regular crops for 2 yr (2005-2006 followed by a ratoon crop for 1 yr (2007. Termites (Odontotermes badius, crickets (Gymnogryllus lucens, and variegated grasshoppers (Zonocerus variegatus were the crop's seedling pests and caused minimal damage. Clavigralla spp. infestations were high at the reproductive stage causing 24% and 29% seed damage in regular and ratoon pigeon pea crops, respectively. Maize slightly suppressed insect pest incidence and damage to pigeon pea pods and seeds but significantly (P La extendida adopción de sistemas de cultivo que son sustentables y benignos para el medio ambiente es vital para la supervivencia macro-económica de la civilización. El intercultivo podría asegurar la estabilidad de población de insectos en un sistema. Un ensayo de campo de 3 anos (2005-2007 fue realizado en Nigeria bajo condiciones de cultivo regular y ratoon para evaluar cinco genotipos de poroto gandul (Cajanus cajan [L.] Huth: ICPL 87, ICPL 161, ICPL 85063, ICP 7120, e ICPL 87119 desde International Crops Research Institute for Semi-Arid Tropics (ICRISAT y una variedad local, por sus rendimientos y susceptibilidades relativas a insectos plaga. Los genotipos de poroto gandul estaban en mezcla con dos genotipos de

  5. Development of cytoplasmic-nuclear male sterility, its inheritance, and potential use in hybrid pigeonpea breeding.

    Science.gov (United States)

    Saxena, Kul B; Ravikoti, V Kumar; Dalvi, Vijay A; Pandey, Lalji B; Gaddikeri, Guruprasad

    2010-01-01

    Pigeonpea [Cajanus cajan (L.) Millsp.] is a unique food legume because of its partial (20-30%) outcrossing nature, which provides an opportunity to breed commercial hybrids. To achieve this, it is essential to have a stable male-sterility system. This paper reports the selection of a cytoplasmic-nuclear male-sterility (CMS) system derived from an interspecific cross between a wild relative of pigeonpea (Cajanus sericeus Benth. ex. Bak.) and a cultivar. This male-sterility source was used to breed agronomically superior CMS lines in early (ICPA 2068), medium (ICPA 2032), and late (ICPA 2030) maturity durations. Twenty-three fertility restorers and 30 male-sterility maintainers were selected to develop genetically diverse hybrid combinations. Histological studies revealed that vacuolation of growing tetrads and persistence of tetrad wall were primary causes of the manifestation of male sterility. Genetic studies showed that 2 dominant genes, of which one had inhibitory gene action, controlled fertility restoration in the hybrids. The experimental hybrids such as TK 030003 and TK 030009 in early, ICPH 2307 and TK 030625 in medium, and TK 030861 and TK 030851 in late maturity groups exhibited 30-88% standard heterosis in multilocation trials.

  6. 木豆毛状根的诱导及其培养条件的研究%Induction and Culture Condition of Hairy Roots of Cajanus cajan (L.) Millsp.

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    于海娣; 王慧梅; 付玉杰

    2012-01-01

    Hairy roots of Cajanus cajan (L. ) Millsp. was induced by Agrobacterium rhizogenes LBA9402 with direct inoculation. The optimum condition of induction of hairy roots in this test was as follows, the leaves of C. cajan were used as explants to be inoculated on 1/2MS solid medium, pre-culture for 2 d, the concentration of bacterium was OD = 0. 6 -0. 8, dissemination for 20 min, co-culture for 3 d, the rate of the hairy roots was 60%. PCR analysis at molecular level proved that T-DNA of Ri plasmid of A. rhizogenes 9402 has been successfully transferred into the genome of hairy roots of C. cajan.%利用发根农杆菌LBA9402对木豆叶片直接进行诱导产生毛状根.本实验研究出诱导木豆毛状根的最佳条件是,以木豆叶片为外植体,于1/2MS固体培养基上预培养2 ~4d,菌液浓度OD600 =0.6 ~0.8,浸染20 min,共培养3d,诱导率为60.00%.在分子水平用PCR检测表明,发根农杆菌9402Ri质粒上的T-DNA成功整合进木豆毛状根的基因组中.

  7. Two Major Clades of Bradyrhizobia Dominate Symbiotic Interactions with Pigeonpea in Fields of Côte d'Ivoire

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    Fossou, Romain K.; Ziegler, Dominik; Zézé, Adolphe; Barja, François; Perret, Xavier

    2016-01-01

    In smallholder farms of Côte d'Ivoire, particularly in the northeast of the country, Cajanus cajan (pigeonpea) has become an important crop because of its multiple beneficial facets. Pigeonpea seeds provide food to make ends meet, are sold on local markets, and aerial parts serve as forage for animals. Since it fixes atmospheric nitrogen in symbiosis with soil bacteria collectively known as rhizobia, C. cajan also improves soil fertility and reduces fallow time. Yet, seed yields remain low mostly because farmers cannot afford chemical fertilizers. To identify local rhizobial strains susceptible to be used as bio-inoculants to foster pigeonpea growth, root nodules were collected in six fields of three geographically distant regions of Côte d'Ivoire. Nodule bacteria were isolated and characterized using various molecular techniques including matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) and DNA sequencing. These molecular analyses showed that 63 out of 85 nodule isolates belonged to two major clades of bradyrhizobia, one of which is known as the Bradyrhizobium elkanii super clade. Phylogenies of housekeeping (16S-ITS-23S, rpoB) and symbiotic (nifH) genes were not always congruent suggesting that lateral transfer of nitrogen fixation genes also contributed to define the genome of these bradyrhizobial isolates. Interestingly, no field-, plant-, or cultivar-specific effect was found to shape the profiles of symbiotic strains. In addition, nodule isolates CI-1B, CI-36E, and CI-41A that belong to distinct species, showed similar symbiotic efficiencies suggesting that any of these strains might serve as a proficient inoculant for C. cajan. PMID:27891120

  8. Environmental Influences on Pigeonpea-Fusarium udum Interactions and Stability of Genotypes to Fusarium Wilt.

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    Sharma, Mamta; Ghosh, Raju; Telangre, Rameshwar; Rathore, Abhishek; Saifulla, Muhammad; Mahalinga, Dayananda M; Saxena, Deep R; Jain, Yogendra K

    2016-01-01

    Fusarium wilt (Fusarium udum Butler) is an important biotic constraint to pigeonpea (Cajanus cajan L.) production worldwide. Breeding for fusarium wilt resistance continues to be an integral part of genetic improvement of pigeonpea. Therefore, the study was aimed at identifying and validating resistant genotypes to fusarium wilt and determining the magnitude of genotype × environment (G × E) interactions through multi-environment and multi-year screening. A total of 976 genotypes including germplasm and breeding lines were screened against wilt using wilt sick plot at Patancheru, India. Ninety two genotypes resistant to wilt were tested for a further two years using wilt sick plot at Patancheru. A Pigeonpea Wilt Nursery (PWN) comprising of 29 genotypes was then established. PWN was evaluated at nine locations representing different agro-climatic zones of India for wilt resistance during two crop seasons 2007/08 and 2008/09. Genotypes (G), environment (E), and G × E interactions were examined by biplot which partitioned the main effect into G, E, and G × E interactions with significant levels (p ≤ 0.001) being obtained for wilt incidence. The genotype contributed 36.51% of resistance variation followed by the environment (29.32%). A GGE biplot integrated with a boxplot and multiple comparison tests enabled us to identify seven stable genotypes (ICPL 20109, ICPL 20096, ICPL 20115, ICPL 20116, ICPL 20102, ICPL 20106, and ICPL 20094) based on their performance across diverse environments. These genotypes have broad based resistance and can be exploited in pigeonpea breeding programs.

  9. AVALIAÇÃO FÍSICA, QUÍMICA, BIOQUÍMICA E AGRONÔMICA DE CULTIVARES DE FEIJÃO-GUANDU (CAJANUS CAJAN (L MILL

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    SOLANGE GUIDOLIM CANNIATTI-BRAZACA

    2009-07-01

    Full Text Available

    RESUMO: Foram estudadas as características físicas, químicas, bioquímicas e agronômicas de 40 cultivares de feijão-guandu (Cajanus cajan (L Mill provenientes da EMBRAPA de São Carlos (SP e originárias de diversas regiões do Brasil e da Índia. Pode-se observar entre os cultivares variações expressivas no tempo de cocção, no teor de lectinas e na produtividade. Os resultados obtidos quanto ao teor protéico e à digestibilidade do guandu, quando comparadas com os resultados obtidos na literatura, mostraram-se semelhantes aos dos grãos de feijão-comum. Pode-se, também, observar baixos teores de taninos entre os cultivares estudados. Foram selecionados três cultivares por apresentarem melhores características entre os parâmetros estudados. PALAVRAS-CHAVE: Feijão-guandu; proteínas; fatores antinutricionais; produtividade.

  10. Potencial de Formigas (Hymenoptera: Formicidae como Agentes Anti-herbívoros em Cultivo de Café (Coffea canephora Pierre e Feijão Guandu [Cajanus cajans (L. Millsp

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    Leandro Chevalier

    2013-07-01

    Abstract. This study analyzed the role of ants as anti-herbivore agents in Coffee (Coffea canephora Pierre and Pigeon Pea [Cajanus cajans (L. Millsp] plants in agroecosystem under organic management. In these plants we used artificial eggs and sugar solution in experiments that simulated the presence of herbivore insects. Coffee plants did not possess any natural attractive that could intensify foraging activity of ants, but pigeon pea plants were infested by treehoppers which attracted ants. Activity of ants was surveyed after sugar solution application and artificial eggs removal was measured 24 and 48 h after start of the experiments. Sugar solution sprinkled on plants foliage increased significantly the activity of ants on coffee and pigeon pea plants. The removal of artificial eggs was significantly greater in branch with ants than without ants for pigeon pea plants. The sugar solution addiction resulted in greater artificial eggs removal in pigeon pea plants, but not in coffee plants. The treatment with sugar solution and presence of treehoppers had similar effects on the activity of ants on the plants. This study showed that the application of sugar solution can be used to increase activity of ants on plants as well as the potential of ants as anti-herbivore agents can be evaluated in experiments that simulate the presence of insects on the plants.

  11. Influência de fatores antinutricionais da leucena (Leucaena leucocephala e Leucaena cunningan e do feijão guandu (Cajanus cajan sobre o epitélio intestinal e o desempenho de frangos de corte

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    Oliveira Paulo Batista de

    2000-01-01

    Full Text Available Um experimento foi conduzido para avaliar a influência de fatores antinutricionais de alguns alimentos sobre o epitélio intestinal e o desempenho de frangos de corte. Foram utilizados 480 pintos de um dia de idade, em um delineamento inteiramente casualizado com quatro tratamentos (A = ração testemunha à base de milho e soja; B = ração contendo Leucaena leucocephala - 6% [LL]; C = ração contendo Leucaena cunningan - 6% [LC] e D = ração contendo feijão guandu - Cajanus cajan - 20% [CC], quatro repetições e 30 aves por unidade experimental. Houve redução de ganho de peso e aumento da conversão alimentar com a utilização de LL e LC, no período de 1a 21 e de 1a 42 dias de idade. Aos 21 dias de idade, observou-se aumento no tamanho dos vilos do jejuno e na relação vilo:cripta do jejuno e íleo, nas aves que receberam LC. Houve aumento significativo (P<0,05 das células caliciformes no duodeno e jejuno em aves de todos os tratamentos e apenas LL e LC aumentaram as células caliciformes do íleo. Aos 42 dias de idade, houve aumento no número de células caliciformes causado por CC no duodeno e por LL, LC e CC no íleo. O consumo de ração e a profundidade de cripta não foram influenciados. A alteração da morfologia da vilosidade do jejuno, bem como a alteração na relação vilo:cripta no período inicial, influenciou negativamente o ganho de peso e a conversão alimentar nos períodos inicial e total.

  12. Comparison of N(2) Fixation and Yields in Cajanus cajan between Hydrogenase-Positive and Hydrogenase-Negative Rhizobia by In Situ Acetylene Reduction Assays and Direct N Partitioning.

    Science.gov (United States)

    La Favre, J S; Focht, D D

    1983-08-01

    Pigeon peas [Cajanus cajan (L.) Millsp.] were grown in soil columns containing (15)N-enriched organic matter. Seasonal N(2) fixation activity was determined by periodically assaying plants for reduction of C(2)H(2). N(2) fixation rose sharply from the first assay period at 51 days after planting to a peak of activity between floral initiation and fruit set. N(2) fixation (acetylene reduction) activity dropped concomitantly with pod maturation but recovered after pod harvests. Analysis of (15)N content of plant shoots revealed that approximately 91 to 94% of plant N was derived from N(2) fixation. The effect of inoculation with hydrogenase-positive and hydrogenase-negative rhizobia was examined. Pigeon peas inoculated with strain P132 (hydrogenase-positive) yielded significantly more total shoot N than other inoculated or uninoculated treatments. However, two other hydrogenase-positive strains did not yield significantly more total shoot N than a hydrogenase-negative strain. The extent of nodulation by inoculum strains compared to indigenous rhizobia was determined by typing nodules according to intrinsic antibiotic resistance of the inoculum strains. The inoculum strains were detected in almost all typed nodules of inoculated plants.Gas samples were taken from soil columns several times during the growth cycle of the plants. H(2) was never detected, even in columns containing pigeon peas inoculated with hydrogenase-negative rhizobia. This was attributed to H(2) consumption by soil bacteria. Estimation of N(2) fixation by acetylene reduction activity was closest to the direct (15)N method when ethylene concentrations in the gas headspace (between the column lid and soil surface) were extrapolated to include the soil pore space as opposed solely to measurement in the headspace. There was an 8-fold difference between the two acetylene reduction assay methods of estimation. Based on a planting density of 15,000 plants per hectare, the direct (15)N fixation rates ranged

  13. Características fisiológicas e de crescimento de cafeeiro sombreado com guandu e cultivado a pleno sol Physiological characteristics and growth of coffee plants grown under shade of pigeonpea and unshaded

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    Heverly Morais

    2003-10-01

    Full Text Available O conhecimento dos efeitos do sombreamento sobre a fisiologia de cafeeiros é importante para se determinar níveis ótimos de radiação e temperatura, bem como para subsidiar estudos sobre o crescimento de plantas sombreadas, a fim de determinar a arquitetura ideal do cafeeiro que maximize a captura da radiação solar disponível em ambientes sombreados. O objetivo deste trabalho foi avaliar características fisiológicas e de crescimento de cafeeiros (Coffea arabica L. cultivados sob sombreamento denso com guandu (Cajanus cajan (L. Millsp. e a pleno sol. O baixo nível de radiação incidente sobre os cafeeiros sombreados com guandu resultou em decréscimos na taxa fotossintética e na transpiração, maior altura de planta, folhas maiores e com menor quantidade de matéria seca. Esses resultados indicam que o excesso de sombra afeta drasticamente a fisiologia e morfologia de C. arabica.The characterization of shade effects on the physiology of coffee is important to determine optimum levels of radiation and temperature, as well as to subsidize studies on growth of shaded plants aiming at determining the ideal coffee plant architecture that maximizes the capture of the available solar radiation in shaded environments. The objective of this work was to evaluate physiological and growth characteristics of coffee (Coffea arabica L. shaded with pigeonpea (Cajanus cajan (L. Millsp. and under full sun. The low level of incident radiation on the coffee shaded with pigeonpea caused decreases in the photosynthetic rate and transpiration, increased plant height and leaf size, but diminished leaf dry matter. These results indicate that the excess of shade drastically affects the physiology and morphology of C. arabica.

  14. Growth of seedlings of pigeon pea (Cajanus cajan (l. millsp, wand riverhemp (Sesbania virgata (cav. pers., and lead tree (Leucaena leucocephala (lam. de wit in an arsenic-contaminated soil

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    Luiz Eduardo Dias

    2010-06-01

    Full Text Available Phytoremediation strategies utilize plants to decontaminate or immobilize soil pollutants. Among soil pollutants, metalloid As is considered a primary concern as a toxic element to organisms. Arsenic concentrations in the soil result from anthropogenic activities such as: the use of pesticides (herbicides and fungicides; some fertilizers; Au, Pb, Cu and Ni mining; Fe and steel production; coal combustion; and as a bi-product during natural gas extraction. This study evaluated the potential of pigeon pea (Cajanus cajan, wand riverhemp (Sesbania virgata, and lead tree (Leucaena leucocephala as phytoremediators of soils polluted by As. Soil samples were placed in plastic pots, incubated with different As doses (0; 50; 100 and 200 mg dm-3 and then sown with seeds of the three species. Thirty (pigeon pea and 90 days after sowing, the plants were evaluated for height, collar diameter and dry matter of young, intermediate and basal leaves, stems and roots. Arsenic concentration was determined in different aged leaves, stems and roots to establish the translocation index (TI between the plant root system and aerial plant components and the bioconcentration factors (BF. The evaluated species showed distinct characteristics regarding As tolerance, since the lead tree and wand riverhemp were significantly more tolerant than pigeon pea. The high As levels found in wand riverhemp roots suggest the existence of an efficient accumulation and compartmentalization mechanism in order to reduce As translocation to shoot tissues. Pigeon pea is a sensitive species and could serve as a potential bioindicator plant, whereas the other two species have potential for phytoremediation programs in As polluted areas. However, further studies are needed with longer exposure times in actual field conditions to reach definite conclusions on relative phytoremediation potentials.A fitorremediação é uma estratégia que utiliza plantas para descontaminar ou imobilizar poluentes

  15. Development of dominant sequence characterized amplified region (SCAR marker linked with plume moth (Exelastis atomosa Walsingham 1886 resistance in pigeon-pea

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    Ramya R Mishra

    2015-12-01

    Full Text Available The mode of gene action governing resistance to plume moth (Exelastis atomosa Walsingham 1886 derived from pigeon-pea (Cajanus scarabaeoides (L. Thouars accession ICPW-94 has been determined and the resistance alleles have been designated as PPM1. The progenies of F2 population and F3 families derived from an interspecific cross C. cajan (L. Huth ('ICP-26' x C. scarabaeoides (accession ICPW-94 revealed monogenic gene action for resistance to plume moth, and the dominant control by single locus or cluster of tightly linked alleles. Bulked segregant analysis (BSA of 116 F2 progenies by using 143 parental polymorphic RAPD primers could identify a fragment OPA09(910 associated with plume moth resistance in coupling phase of linkage. Further single plant analysis of the 116 F2 mapping population revealed OPA09(910 was linked to PPMi locus conferring host resistance to plume moth with recombination fraction (rf value of 0.125 (12.7 cM of Kosambi function. The resistance specific fragment OPA09(910 was cloned, sequenced and converted into a sequence characterized amplified region (SCAR marker, SCOPA09(942, which was also closely associated (10.3 cM with the locus PPMl with rf value 0.102. BLAST analysis with pigeon-pea genome sequence also confirmed its occurrence in CcLG02 (Scafseq.LG_V5.0fa and contig 01597 (AFSP01.fsa1. This SCAR marker showed reasonable screening efficiency in the F2, F3, and BC1F1 lines, thus it can be used as genetic handle in marker-assisted introgression of the genomic fragment conferring plume moth resistance and screening of breeding lines in pigeon-pea.

  16. 木豆叶醇提物对皮质酮诱导的PC12细胞损伤的保护作用%Protective Effect of Alcohol Extract of Cajanus cajan on Corticosterone-induced Lesion in Cultured PC12 Cells

    Institute of Scientific and Technical Information of China (English)

    姜保平; 刘亚旻; 李宗阳; 宋波; 潘瑞乐

    2012-01-01

    建立皮质酮诱导的PC12细胞损伤模型并观察木豆叶醇提物及不同组分对皮质酮损伤PC12细胞的保护作用.以100μ mol/L的皮质酮诱导PC12细胞损伤;损伤后的PC12细胞与木豆叶醇提物及不同组分孵育24h,通过形态学观察、MTT检测、LDH测定,研究各组分对皮质酮损伤PC12细胞的保护作用.结果表明,PC12细胞与皮质酮孵育48 h后细胞存活率明显降低,而LDH水平显著升高.而加入木豆叶醇提物及各组分时上述效果明显减轻,且存在明显的剂量依赖关系.从以上结果可知,木豆叶醇提物及不同组分对皮质酮损伤的PC12细胞均有保护作用,且醇提物的效果最好.%The aim of this paper was to establish the corticosterone-induced damage model in PC12 cells and study the protective activity of alcohol extracts and its fractions from Cajanus cajan on corticosterone-induced PC12 cells. PC12 cells were exposed to different concentrations of corticosterone to determine the optimum concentration of establishing the damage model,and then the injured PC12 cells were incubated with the alcohol extract and its fractions for 24 h. After that the neuroprotective effect on injured PC12 cells was investigated by morphological observation, LDH detection and MTT determination. The results showed that the survival rate of PC12 cells and the cells exposed to corticosterone (100 (μmol/L) for 48 h decreased obviously,with an increase in lactate dehydrogenase (LDH) level,but the effect became weak when the alcohol extracts and its fractions from the C. cajan was added to the PC 12 cells,and it was in a dose-dependent manner. The author suggests that the alcohol extract and its fractions from C. cajan can generate a neuroprotective effect on injured PC 12 cells induced by corticosterone,and that of alcohol extract is the most significant.

  17. Cytoplasmic male sterile systems in pigeonpea with special reference to A7 CMS

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    Nalini Mallikarjuna, Deepak R Jadhav, Kulbushan B. Saxena and Rakesh K. Srivastava

    2012-12-01

    Full Text Available Compared to the cereals and vegetable crops, cytoplasmic male sterility is comparatively a new system available topigeonpea breeders to exploit heterosis/hybrid vigor. It has been possible to develop a CMS system for pigeonpea as crosspollination is possible in this crop. Various CMS systems have been developed for pigeonpea utilizing different wildrelatives from different gene pools. CMS based hybrids developed from A4 system have recorded superiority in yieldscompared to the checks on farmer’s fields. This has opened up a new window for yield improvement in pigeonpea. Thepaper discusses different CMS systems available for pigeonpea in general and the A7 system which was developed utilizingCajanus platycarpus, in particular.

  18. Accumulation effects on function of the high-brush forest compound model of the 'Tamarindus+Cajanus cajan'%"罗望子+木豆"乔灌复合模式林水土保持功能的叠加效应初探

    Institute of Scientific and Technical Information of China (English)

    拜得珍; 纪中华; 沙毓沧; 赵艳萍; 潘志贤

    2006-01-01

    以降水等级为主要影响因子,研究了罗望子(Tamarindus indica)+木豆(Cajanus cajan)乔灌复合模式林对降雨的截留、贮存所产生的水保效应.结果表明:罗望子人工林冠层和复合木豆人工灌木林林冠层年截留降雨量分别为97.99和145.4 mm,分别占全年降雨量水的17.48 %和25.86 %;罗望子人工林的树干茎流率占大气降水的2.37 %;复合模式林枯枝落叶的蓄积量为7.08 t/hm2,对降水的平均截留率为12.47 %,其最大持水量相当于1.8 mm的降水;模式林表层土壤(0~40 cm)滞留水分能力相当于2.7 mm降雨,贮存土壤水分能力可达1553.2 t/hm2,产生径流的最小降雨量为35.4 mm,其侵蚀模数和径流模数比对照减少98.52 %和93.21 %,体现出了良好的水土保持和水源涵养效应.

  19. Genome-wide dissection of AP2/ERF and HSP90 gene families in five legumes and expression profiles in chickpea and pigeonpea.

    Science.gov (United States)

    Agarwal, Gaurav; Garg, Vanika; Kudapa, Himabindu; Doddamani, Dadakhalandar; Pazhamala, Lekha T; Khan, Aamir W; Thudi, Mahendar; Lee, Suk-Ha; Varshney, Rajeev K

    2016-07-01

    APETALA2/ethylene response factor (AP2/ERF) and heat-shock protein 90 (HSP90) are two significant classes of transcription factor and molecular chaperone proteins which are known to be implicated under abiotic and biotic stresses. Comprehensive survey identified a total of 147 AP2/ERF genes in chickpea, 176 in pigeonpea, 131 in Medicago, 179 in common bean and 140 in Lotus, whereas the number of HSP90 genes ranged from 5 to 7 in five legumes. Sequence alignment and phylogenetic analyses distinguished AP2, ERF, DREB, RAV and soloist proteins, while HSP90 proteins segregated on the basis of their cellular localization. Deeper insights into the gene structure allowed ERF proteins to be classified into AP2s based on DNA-binding domains, intron arrangements and phylogenetic grouping. RNA-seq and quantitative real-time PCR (qRT-PCR) analyses in heat-stressed chickpea as well as Fusarium wilt (FW)- and sterility mosaic disease (SMD)-stressed pigeonpea provided insights into the modus operandi of AP2/ERF and HSP90 genes. This study identified potential candidate genes in response to heat stress in chickpea while for FW and SMD stresses in pigeonpea. For instance, two DREB genes (Ca_02170 and Ca_16631) and three HSP90 genes (Ca_23016, Ca_09743 and Ca_25602) in chickpea can be targeted as potential candidate genes. Similarly, in pigeonpea, a HSP90 gene, C.cajan_27949, was highly responsive to SMD in the resistant genotype ICPL 20096, can be recommended for further functional validation. Also, two DREB genes, C.cajan_41905 and C.cajan_41951, were identified as leads for further investigation in response to FW stress in pigeonpea.

  20. Carbon dioxide and light responses of photosynthesis in cowpea and pigeonpea during water deficit and recovery

    Energy Technology Data Exchange (ETDEWEB)

    Lopez, F.B.; Setter, T.L.; McDavid, C.R.

    1987-10-01

    Greenhouse-grown pigeonpea (Cajunus cajan, (L.)) and cowpea (Vigna unguiculata, (L.)) were well-watered or subjected to low water potential by withholding water to compare their modes of adaptation to water-limited conditions. Leaf CO/sub 2/ exchange rate (CER), leaf diffusive conductance to CO/sub 2/ (g/sub L/), and CO/sub 2/ concentration in the leaf intercellular air space (C/sub i/) were determined at various CO/sub 2/ concentrations and photon flux densities (PFD) of photosynthetically active radiation. In cowpea, g/sub L/ declined to less than 15% of controls and total water potential (Psi/sub w/) at midafternoon declined to -0.8 megapascal after 5 days of withholding water, whereas g/sub L/ in pigeonpea was about 40% of controls even though midafternoon Psi/sub w/ was -1.9 megapascal. After 8 days of withholding water, Psi/sub w/ at midafternoon decline to -0.9 and -2.4 megapascals in cowpea and pigeonpea, respectively. The solute component of water potential (Psi/sub s/) decreased substantially less in cowpea than pigeonpea. Photosynthetic CER at saturation photon flux density (PFD) and ambient external CO/sub 2/ concentration on day 5 of withholding decreased by 83 and 55% in cowpea and pigeonpea, respectively. When measured at external, CO/sub 2/ concentration in bulk air of 360 microliters per liter, the CER of cowpea had fully recovered to control levels 3 days after rewatering; however, at 970 microliters per liter the PFD-saturated CERS of both species were substantially lower than in controls, indicating residual impairment.

  1. Prospects of Pigeonpea Hybrids in Indian Agriculture

    Directory of Open Access Journals (Sweden)

    KB Saxena and N Nadarajan

    2010-07-01

    Full Text Available Stagnant production, soaring prices, and enhanced imports of pigeonpea (red gram have been matter of concern to the primestakeholders in India. A new hybrid pigeonpea breeding technology, developed jointly by the International Crops ResearchInstitute for the Semi-arid Tropics (ICRISAT and Indian Council of Agriculture Research (ICAR is capable of substantiallyincreasing the productivity of red gram, and thus offering hope of pulse revolution in the country. The hybrid technology,based on cytoplasmic nuclear male-sterility (CMS system, has given an opportunity of achieving the long-cherished goal ofbreaking yield barrier in pigeonpea. In the past two years ICRISAT and ICAR have tested over 1000 experimental hybridsand among these GTH 1 and ICPH 2671 were found the most outstanding. GTH-1 has yielded 32% more yield than bestlocal variety, GT 101. ICPH 2671 is highly resistant to fusarium wilt and sterility mosaic diseases and produced 38% moreyield over the popular variety Maruti in multi-location trials conducted for over four years. In the on-farm trials conducted inthe states of Maharashtra, Karnataka, Andhra Pradesh, Madhya Pradesh, and Jharkhand during 2007, 2008 and 2009 havedemonstrated 30% yield advantage over local check varieties. So far the progress in the mission of enhancing theproductivity of pigeonpea has been encouraging and the reality of commercial hybrids is just around the corner. The newhybrid pigeonpea will serve as the platform for the tremendous growth of pulse production in India.

  2. Pigeonpea

    NARCIS (Netherlands)

    Claessens, L.F.G.; Gowda, C.L.L.; Craufurd, P.

    2012-01-01

    The document attempts to distil what is currently known about the likely impacts of climate change on the commodities and natural resources that comprise the mandate of CGIAR and its 15 Centres. It was designed as one background document for a review carried out by the High Level Panel of Experts on

  3. Pigeonpea

    NARCIS (Netherlands)

    Claessens, L.F.G.; Gowda, C.L.L.; Craufurd, P.

    2012-01-01

    The document attempts to distil what is currently known about the likely impacts of climate change on the commodities and natural resources that comprise the mandate of CGIAR and its 15 Centres. It was designed as one background document for a review carried out by the High Level Panel of Experts on

  4. Utilization of Decorticated Pigeon Pea (Cajanus cajan L. With Wheat (Triticum aestivum Flours in Bread Making

    Directory of Open Access Journals (Sweden)

    H.A. Hassan

    2011-12-01

    Full Text Available The objectives of this study were to investigate the use of decorticated pigeon pea flour in the development of protein rich - bread, suitable for general and specific nutritional purposes and to study the effect of incorporation of pigeon pea flour on the sensory evaluation and quality of bread produced. Decorticated Pigeon Pea Flour (DPPF was incorporated with wheat flour (WF 72% Ext. to replace 0, 5, 10, 15, 20 and 25% of the wheat flour for bread making. Proximate composition, falling number, gluten quality and quantity, tannins and phytic acid were determined for the flour blends (Composite flour. Bread proximate composition, sensory evaluation and specific volume were determined as well. Decortication of pigeon pea led to decrease in moisture, ash, tannins and phytic acid and increase in the protein and carbohydrates contents. The falling number (alpha amylase activity significantly increased over the control with the increasing level of DPPF. There were also significant reduction (p#0.05 on gluten quantity (wet and dry gluten and quality (gluten index. No significant differences were found in bread specific volume up to 10% addition of DPPF. The protein, ash, fat contents and calorific values for the bread were significantly increased (p#0.05 with incorporation of DPPF. Increasing levels of the replacement of DPPF resulted in a decrease in the organoleptic quality of the bread. The bread containing up to 15% DPPF was found to be the best in overall acceptability.

  5. Cultivar preference and sensory evaluation of vegetable pigeon pea (Cajanus cajan) in Eastern Kenya

    Science.gov (United States)

    Preference and acceptability of twelve vegetable pigeon pea genotypes of medium maturity was evaluated in Eastern Kenya based on six seed cultivar parameters of color, appearance, taste, odor, tenderness and overall seed acceptability. The sensory characteristics were scored by consumers and farmers...

  6. Descrição botânica de linhagens puras selecionadas de guandu Botanical description of selected pigeon-pea pure lines

    Directory of Open Access Journals (Sweden)

    Milena Provazi

    2007-04-01

    Full Text Available Este estudo foi desenvolvido com o objetivo de realizar a descrição botânica de 11 linhagens de Cajanus cajan. Os principais descritores foram: hábito de crescimento, altura de plantas (cm, número de ramos, diâmetro do caule (mm, cor básica da flor, cor básica da vagem, cor das sementes, cor em volta do hilo das sementes, largura do hilo e forma das sementes. Os descritores utilizados mostraram-se suficientes para caracterizar as linhagens com características distintas, pelas quais podem ser facilmente identificadas.This work aimed to perform the morphologic characterization of eleven previously selected Cajanus cajan lines. The main descriptors were: plant height (cm, number of branches, stem diameter (mm, basic flower color, basic pod color, seed color, seed hilum color, hilum thickness, and seed shape. The descriptors used were sufficient to characterize the lines with distinct characteristics. Using these descriptors the lines may be easily identified.

  7. Quantification of Atmospheric N2 Fixed by Cowpea, Pigeonpea and ...

    African Journals Online (AJOL)

    In the intercrop system cowpea, pigeonpea and greengram fixed 16, 4, and 24 kg N2, respectively. In both cases, the ..... rotation (with the legume) should be adopted for maximizing .... benefits from biological nitrogen fixation: An ecological ...

  8. Effect of insecticide application on pests of late maturing pigeonpea ...

    African Journals Online (AJOL)

    Effect of insecticide application on pests of late maturing pigeonpea cultivar ... University of Nigeria, Nsukka agro-ecological zone in 2000/2001 cropping season. ... control of pigoenpea pests under the integrated management concept.

  9. Survey on pigeon pea production systems, utilization and marketing in semi-arid lands of Kenya

    Directory of Open Access Journals (Sweden)

    Baudoin J.P.

    2001-01-01

    Full Text Available In order to assess the status of pigeonpea (Cajanus cajan (L. Millsp. production in Kenya, two surveys were carried out in Makueni and Mbeere Districts in areas representative of the main agro-ecological pigeonpea producing zone of the country : (Mid-altitude ASAL. Participatory Rural Appraisal (PRA approach was chosen as research method and was completed by household interviews based on a semi-structured questionnaire. The main points developed are the presentation of the different farming systems in which pigeonpea is considered as an important legume crop, the identification of the factors explaining pigeonpea production variations, the quantification of the use of improved varieties and improved production practices, and the analysis of the major patterns and trends in pigeonpea production, consumption and marketing.

  10. Quality evaluation of stiff porridges prepared from Irish potato (Solanum tuberosum) and pigeon pea (Cajanus cajan) starch blends.

    Science.gov (United States)

    Abu, Joseph Oneh; Enyinnaya, Chinma Chiemela; James, Samaila; Okeleke, Ezinne

    2012-06-01

    Quality attributes of stiff porridges prepared from Irish potato and pigeon pea starch blends were studied. Starches were extracted from Irish potato and pigeon pea using a wet extraction method. Various ratios of the starches were mixed and analyzed for chemical, functional and pasting properties. The starch blends were then prepared into stiff porridges for sensory evaluation using a 20-man sensory panel. Substitution of Irish potato starch with pigeon pea starch led to increases in protein (0.15 to 1.2%), fat (0.26 to 0.56%) and ash (0.30 to 0.69%) while the amylose content of the starch blends decreased (from 23.8 to 18.4%) respectively. Functional properties such as bulk density (0.75 to 0.60 g/cm(3)), water absorption capacity (3.1 to 2.6 g water/ g sample) and dispersibility (58.6 to 42.7%) decreased significantly (P stiff porridges were not adversely affected by pigeon pea starch inclusion. Therefore it should be possible to incorporate up to 50% of low digestible pigeon pea starch into Irish potato starch from legumes such as pigeon pea as alternatives to cassava starch in the preparation of stiff porridges. Such porridges made from Irish potato and legume starches could provide additional incentive for individuals requiring decreased and or slow starch digestibility such as diabetics.

  11. DIGESTIBILIDAD DE LA HARINA DE GUANDÚL (Cajanus Cajan EN ALIMENTACIÓN DE POLLOS DE ENGORDE

    Directory of Open Access Journals (Sweden)

    JULIANA ISABEL CARVAJAL-TAPIA

    Full Text Available Con el objetivo de evaluar la digestibilidad aparente del alimento y la proteína de dietas con inclusión de harina de grano de guandúl como fuente alternativa de alimentación para pollos de engorde, se utilizaron 96 animales machos recriados de la línea Cobb 500 distribuidos en 16 jaulas metabólicas, empleando un diseño experimental completamente al azar (DCA con cuatro tratamientos, cuatro repeticiones y seis animales por unidad experimental, donde los tratamientos fueron 4 dietas iso-protéicas e iso-energéticas con niveles de inclusión de 0, 10, 20 y 30% de harina de guandúl. En los resultados se observó diferencias estadísticas significativas (p<0,05 en la etapa inicial, los tratamientos T0 y T1 fueron estadísticamente diferentes a los tratamientos con mayor inclusión de guandúl T2 y T3. En la etapa final los tratamientos T0 y T1 fueron iguales estadísticamente diferenciándose de los tratamientos T2 y T3 entre los cuales no se presentó diferencias estadísticas significativas. En la etapa final de la alimentación de pollos se puede incluir hasta el 10% de harina de guandúl como fuente protéica sin presentar diferencias estadísticas significativas de las digestibilidades aparentes del alimento y de la proteína.

  12. Development of a potential functional food prepared with pigeon pea (Cajanus cajan), oats and Lactobacillus reuteri ATCC 55730.

    Science.gov (United States)

    Barboza, Yasmina; Márquez, Enrique; Parra, Katynna; Piñero, M Patricia; Medina, Luis M

    2012-11-01

    The purpose of this study was to investigate the survival of Lactobacillus reuteri ATCC 55730 in creams, prepared with pigeon peas and oat. Products were analysed to determine their content of protein, fibre, fat, carbohydrates and degree of likeness. Viable numbers of L. reuteri and pH were determined after 1, 7, 14, 21 and 28 days of storage at 4°C. Results showed significant differences (P 0.05) were found on sensory quality between control and creams with L. reuteri. After 28 days, the cell viability was above 7 log cfu/g in all creams. L. reuteri ATCC 55730 had the highest viability in cream with 40% pigeon pea and 20% oat (8.16 log cfu/g). In conclusion, due to its acceptability and highly nutritious value, the product could be used so as to support the growth of L. reuteri.

  13. Respuesta de Gallinas Ponedoras a Raciones con Diferentes Niveles de Harina de Hojas de Guandul (CajanusCajan L.

    Directory of Open Access Journals (Sweden)

    Ruíz V. Juan Guillermo

    1969-09-01

    Full Text Available En el presente trabajo se dan los resultados de dos ensayos llevados a cabo en el Centro Nacional de Investigaciones Agropecuarias de Medellín, para estudiar el efecto producido por la harina de hojas de guandul(Cajanuscajan en la coloración de la yema y en la producción de ponedoras. Durante el primer ensayo se trabajó con 120 gallinas Hy-line en postura avanzada, usando 3 raciones con 74%, 70.5% y68.5%de maíz amarillo como material prima básica y niveles de 0%, 6% y 9% de harina de guandul respectivamente. Aunque a medida que se aumentó el nivel de guandul hubo una pequeña baja en la producción de huevos, esta diferencia no fue significativa, como tampoco lo fue el (consumo de alimento y la coloración de la yema. Esta última fue prácticamente igual en los 3 lotes, debido al alto contenido de maíz amarillo en cada una de las raciones. Para el segundo ensayo se trabajó con 160 gallinas de igual raza, pero con 2 -3 meses de postura y 4 tratamientos: 3 en base a sorgo con 0%, 9% Y 12 % de harina de guandul y un cuarto lote con ración comercial, molida para evitar diferencias. La producción de huevos presentó una diferencia significativamente superior, al nivel 0 .05 %, de los lotes 0%y comercial sobre 9% y 12%. En cuanto al consumo de alimento se encontró diferencia altamente significativa del lote comercial sobre los otros 3 lotes. También el comercial fue el de mayor consumo de alimento por docena de huevos producidos. La coloración de la yema del huevo del lote 0% de guandul fue nula obteniendo un producto de difícil mercado. En los lotes 9%, 12% y comercial fue apropiada y muy semejante la pigmentación. El alimento por docena de huevo durante el segundo ensayo fue mayor en el lote con ración comercial, luego 12 %, 9 % y 0% respectivamente. A los siete días se produjo la pigmentación adecuada en la yema del huevo. Huevos almacenados por 25 días no perdieron su grado de coloración, pero sí su consistencia.

  14. Molecular cloning and characterization of the light-harvesting chlorophyll a/b gene from the pigeon pea (Cajanus cajan).

    Science.gov (United States)

    Qiao, Guang; Wen, Xiao-Peng; Zhang, Ting

    2015-12-01

    Light-harvesting chlorophyll a/b-binding proteins (LHCB) have been implicated in the stress response. In this study, a gene encoding LHCB in the pigeon pea was cloned and characterized. Based on the sequence of a previously obtained 327 bp Est, a full-length 793 bp cDNA was cloned using the rapid amplification of cDNA ends (RACE) method. It was designated CcLHCB1 and encoded a 262 amino acid protein. The calculated molecular weight of the CcLHCB1 protein was 27.89 kDa, and the theoretical isoelectric point was 5.29. Homology search and sequence multi-alignment demonstrated that the CcLHCB1 protein sequence shared a high identity with LHCB from other plants. Bioinformatics analysis revealed that CcLHCB1 was a hydrophobic protein with three transmembrane domains. By fluorescent quantitative real-time polymerase chain reaction (PCR), CcLHCB1 mRNA transcripts were detectable in different tissues (leaf, stem, and root), with the highest level found in the leaf. The expression of CcLHCB1 mRNA in the leaves was up-regulated by drought stimulation and AM inoculation. Our results provide the basis for a better understanding of the molecular organization of LCHB and might be useful for understanding the interaction between plants and microbes in the future.

  15. Effect of pigeon pea (Cajanus cajan L.) on high-fat diet-induced hypercholesterolemia in hamsters.

    Science.gov (United States)

    Dai, Fan-Jhen; Hsu, Wei-Hsuan; Huang, Jan-Jeng; Wu, She-Ching

    2013-03-01

    Obesity is associated with increased systemic and airway oxidative stress, which may result from a combination of adipokine imbalance and antioxidant defenses reduction. Obesity-mediated oxidative stress plays an important role in the pathogenesis of dyslipidemia, vascular disease, and nonalcoholic hepatic steatosis. The antidyslipidemic activity of pigeon pea were evaluated by high-fat diet (HFD) hamsters model, in which the level of high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), total cholesterol (TC), and total triglyceride (TG) were examined. We found that pigeon pea administration promoted cholesterol converting to bile acid in HFD-induced hamsters, thereby exerting hypolipidemic activity. In the statistical results, pigeon pea significantly increased hepatic carnitine palmitoyltransferase-1 (CPT-1), LDL receptor, and cholesterol 7α-hydroxylase (also known as cytochrome P450 7A1, CYP7A1) expression to attenuate dyslipidemia in HFD-fed hamsters; and markedly elevated antioxidant enzymes in the liver of HFD-induced hamsters, further alleviating lipid peroxidation. These effects may attribute to pigeon pea contained large of unsaturated fatty acids (UFA; C18:2) and phytosterol (β-sitosterol, campesterol, and stigmasterol). Moreover, the effects of pigeon pea on dyslipidemia were greater than β-sitosterol administration (4%), suggesting that phytosterone in pigeon pea could prevent metabolic syndrome.

  16. Genomics for greater efficiency in pigeonpea hybrid breeding

    Directory of Open Access Journals (Sweden)

    Rachit K Saxena

    2015-10-01

    Full Text Available Cytoplasmic genic male sterility based hybrid technology has demonstrated its immense potential in increasing the productivity of various crops, including pigeonpea. This technology has shown promise for breaking the long-standing yield stagnation in pigeonpea. There are difficulties in commercial hybrid seed production due to non-availability of field-oriented technologies such as time-bound assessment of genetic purity of hybrid seeds. Besides this, there are other routine breeding activities which are labour oriented and need more resources. These include breeding and maintenance of new fertility restorers and maintainer lines, diversification of cytoplasm, and incorporation of biotic and abiotic stress resistances. The recent progress in genomics research could accelerate the existing traditional efforts to strengthen the hybrid breeding technology. Marker based seed purity assessment, identification of heterotic groups; selection of new fertility restorers are few areas which have already been initiated. In this paper efforts have been made to identify critical areas and opportunities where genomics can play a leading role and assist breeders in accelerating various activities related to breeding and commercialization of pigeonpea hybrids.

  17. Boric acid and boronic acids inhibition of pigeonpea urease.

    Science.gov (United States)

    Reddy, K Ravi Charan; Kayastha, Arvind M

    2006-08-01

    Urease from the seeds of pigeonpea was competitively inhibited by boric acid, butylboronic acid, phenylboronic acid, and 4-bromophenylboronic acid; 4-bromophenylboronic acid being the strongest inhibitor, followed by boric acid > butylboronic acid > phenylboronic acid, respectively. Urease inhibition by boric acid is maximal at acidic pH (5.0) and minimal at alkaline pH (10.0), i.e., the trigonal planar B(OH)3 form is a more effective inhibitor than the tetrahedral B(OH)4 -anionic form. Similarly, the anionic form of phenylboronic acid was least inhibiting in nature.

  18. Association of nad7a Gene with Cytoplasmic Male Sterility in Pigeonpea

    Directory of Open Access Journals (Sweden)

    Pallavi Sinha

    2015-07-01

    Full Text Available Cytoplasmic male sterility (CMS has been exploited in the commercial pigeonpea [ (L. Millsp.] hybrid breeding system; however, the molecular mechanism behind this system is unknown. To understand the underlying molecular mechanism involved in A CMS system derived from (Haines Maesen, 34 mitochondrial genes were analyzed for expression profiling and structural variation analysis between CMS line (ICRISAT Pigeonpea A line, ICPA 2039 and its cognate maintainer (ICPB 2039. Expression profiling of 34 mitochondrial genes revealed nine genes with significant fold differential gene expression at ≤ 0.01, including one gene, , with 1366-fold higher expression in CMS line as compared with the maintainer. Structural variation analysis of these mitochondrial genes identified length variation between ICPA 2039 and ICPB 2039 for (subunit of gene. Sanger sequencing of and genes in the CMS and the maintainer lines identified two single nucleotide polymorphisms (SNPs in upstream region of and a deletion of 10 bp in in the CMS line. Protein structure evaluation showed conformational changes in predicted protein structures for between ICPA 2039 and ICPB 2039 lines. All above analyses indicate association of gene with the CMS for A cytoplasm in pigeonpea. Additionally, one polymerase chain reaction (PCR based Indel marker ( has been developed and validated for testing genetic purity of A derived CMS lines to strengthen the commercial hybrid breeding program in pigeonpea.

  19. Aspectos tecnológicos e sensoriais do guandu [Cajanus cajan (L.) Millsp.] enlatado em diferentes estádios de maturação

    OpenAIRE

    1999-01-01

    O objetivo deste trabalho é viabilizar a introdução do guandu verde enlatado no mercado brasileiro. Foram realizadas três colheitas de grãos ainda verdes da cultivar IAC Fava Larga obtidos no 44º, 57º e 62º dia após a floração (DAF) e finalmente no 92º DAF, quando os grãos já possuíam cor pardo-amarelada. Para todos os lotes, após otimização das condições de enlatamento, 121ºC durante 5 a 6 minutos, efetuaram-se estudos quanto ao acúmulo de matéria seca, composição dos grãos, cor, textura e a...

  20. Nutrients and certain lipid soluble bioactive components in dehusked whole grains (gota) and dehusked splits (dhal) from pigeon pea (Cajanus cajan) and their cooking characteristics.

    Science.gov (United States)

    Jayadeep, Padmanabhan A; Sashikala, Vadakkoot B; Pratape, Vishwas M

    2009-01-01

    The nutritional quality of dehusked whole grains (gota) and dehusked splits (dhal) in red and white varieties of pigeon pea regarding proximate composition and certain lipid-soluble bioactive components was investigated. A decrease in fat and crude fiber was noticed when gota was converted to dhal. The lipid profile of gota and dhal from red and white husk pigeon pea types indicated that essential fatty acids were greater in gota than in their respective dhals. Gota from white husk variety contained more tocopherols than the red variety. Dhal contained less tocopherols than gota. A decrease in the content of gamma and alpha tocopherols, vitamin E activity and total antioxidant activity also indicates loss of bioactive components on splitting gota into dhal. Cooking time and dispersed solids on cooking indicated good cooking quality of gotta. The results indicated the nutritional superiority of gota over dhal and its similarity with dhal in cooking characteristics.

  1. Effect of incorporation of decorticated pigeon pea (Cajanus cajan protein isolate on functional, baking and sensory characteristics of Wheat (Triticum aesitivum biscuit

    Directory of Open Access Journals (Sweden)

    H. A. Hassan

    2013-08-01

    Full Text Available This study was undertaken with the objectives of using the decorticated pigeon pea protein isolate in the development of protein rich-biscuit, suitable for general and specific nutritional purposes and to study the effect of incorporation of pigeon pea protein isolate on the sensory evaluation and quality of biscuit produced. Decorticated Pigeon Pea protein Isolate (DPPI was incorporated in wheat (Triticum aesitivum flour (WF, extraction rate 72%, for making fortified biscuit. Ratios of DPPI in wheat flour were adjusted to protein levels of 15, 20 and 25%, respectively. Rheological and functional properties as well as proximate composition, nutritive value and sensory characteristics for the biscuit produced were assessed. The gluten quantity (dry and wet and falling number of wheat flour were significantly (p#0.05 decreased with the incorporation of DPPI from 10.25 to 7.6%; from 31.2 to 22.5% and from 657 to 443 sec., for 0,15, 20 and 25% protein levels, respectively.Water Retention Capacity (WRC, Bulk Density (BD and Fat Absorption Capacity (FAC were obtained. Addition of DPPI resulted in an increase in water absorption which was found to be ranged from 66.7 to 71.0%; dough development time from 4.5 to 7.3 min and dough stability 1.7 to 5.8 min. Biscuit supplemented with DPPI showed significant increase (p≤0.05 in ash, protein with high level of incorporation and significant decrease (p≤0.05 in carbohydrates and caloric values when biscuit wheat was supplemented with high level of DPPI. Incorporation of DPPI showed no significant differences (p≤0.05 on biscuit spread ratios compared to wheat biscuit (control. Biscuit with 15% protein level was found to be superior in all its sensory characteristics compared to the other blends.

  2. An Economic Analysis of Pigeonpea Seed Production Technology and Its Adoption Behavior: Indian Context

    Directory of Open Access Journals (Sweden)

    Govind Pal

    2016-01-01

    Full Text Available The present study was based on primary data collected from 100 farmers in Gulbarga district of Karnataka, India, during the agricultural year 2013-2014. Study shows that average land holding size of pigeonpea seed farmers was higher in comparison to grain farmers and district average. The study illustrates a ratio of 32 : 68 towards fixed and variable costs in pigeonpea certified seed production with a total cost of ₹ 39436 and the gross and net returns were ₹ 73300 and ₹ 33864 per hectare, respectively. The total cost of cultivation, gross return, and net return in pigeonpea seed production were higher by around 23, 32, and 44 percent than grain production, respectively. Hence, production of certified seed has resulted in a win-win situation for the farmers with higher yield and increased returns. The decision of the farmer on adoption of seed production technology was positively influenced by his education, age, land holding, irrigated land, number of crops grown, and extension contacts while family size was influencing negatively. Higher yield and profitability associated with seed production can be effectively popularized among farmers, resulting in increased certified seed production.

  3. Single Nucleotide Polymorphism Genotyping for Breeding and Genetics Applications in Chickpea and Pigeonpea using the BeadXpress Platform

    Directory of Open Access Journals (Sweden)

    Manish Roorkiwal

    2013-07-01

    Full Text Available Single nucleotide polymorphisms (SNPs are ideal molecular markers due to their higher abundance. Although several types of genotyping platforms for assaying large number of SNPs are available, in cases such as marker-assisted selection, where few markers are required for genotyping a set of potential lines, high-throughput SNP genotyping platforms (e.g., iScan or Infinium may not be cost effective. In this scenario, GoldenGate assays based on VeraCode technology using Illumina BeadXpress seems to be the most cost-effective platform. The objective of this study was to develop cost-effective SNP genotyping platforms in chickpea ( L. and pigeonpea ( L.. Two sets of SNPs, one each for chickpea (96 SNPs and pigeonpea (48 SNPs, were developed and tested by genotyping 288 diverse genotypes from respective reference sets. The SNPs selected for the oligo pool assays had high transferability to crop wild relative species. The mean polymorphism information content value of assayed SNP markers was 0.31 and 0.32 in chickpea and pigeonpea, respectively. No unique pattern was observed in the chickpea reference set whereas two major groups were observed in the case of the pigeonpea reference set. The Illumina BeadXpress platform assays developed for chickpea and pigeonpea are highly informative and cost effective for undertaking genetic studies in these legume species.

  4. Aspectos tecnológicos e sensoriais do guandu [Cajanus cajan (L. Millsp.] enlatado em diferentes estádios de maturação Technological and sensorial aspects of pigeon pea [Cajanus cajan (L. Millsp.] canned at different stages of maturation

    Directory of Open Access Journals (Sweden)

    M.F.P. BARCELOS

    1999-01-01

    Full Text Available O objetivo deste trabalho é viabilizar a introdução do guandu verde enlatado no mercado brasileiro. Foram realizadas três colheitas de grãos ainda verdes da cultivar IAC Fava Larga obtidos no 44º, 57º e 62º dia após a floração (DAF e finalmente no 92º DAF, quando os grãos já possuíam cor pardo-amarelada. Para todos os lotes, após otimização das condições de enlatamento, 121ºC durante 5 a 6 minutos, efetuaram-se estudos quanto ao acúmulo de matéria seca, composição dos grãos, cor, textura e aceitação sensorial. Conclui-se, através do acúmulo de matéria seca do grão, que a maturidade fisiológica ocorreu em torno do 62º DAF, quando também apresentou as melhores características para o enlatamento. As cores dos grãos pós-enlatamento, vermelha e amarela (unidades Lab Hunter apresentaram estáveis até o 62º DAF. A textura foi gradativamente mais firme com o avanço do amadurecimento tendo verificado correlação positiva (r = 0,96 entre medidas de texturas sensorial e instrumental. A aceitação do guandu enlatado cultivar IAC Fava Larga foi apenas regular.The objective of this work is to enable the introduction of canned green pigeon pea in Brazilian market. Three harvests of still green grains of the cultivar IAC Fava Larga, obtained on the 44th, 57th and 62nd days after flowering, DAF, and finally on the 92nd DAF when the grains already possessed yellowish dusky color, were undertaken. To all the lots, after optimization of canning conditions, 121ºC for 5 to 6 minutes, studies concerning the accumulation of dry matter, grain composition, color, texture and sensorial acceptance were carried out. It was concluded through the accumulation of dry matter of the grain, that the physiological maturity occurred around the 62nd DAF. The texture was gradually firmer with advancing maturation, and positive correlation having been found (r = 0,96 between sensorial and instrumental texture measures was noticed. The acceptance of the canned pigeon pea cultivar IAC Fava Larga was only regular.

  5. Aspectos tecnológicos e sensoriais do guandu [Cajanus cajan (L.) Millsp.] enlatado em diferentes estádios de maturação Technological and sensorial aspects of pigeon pea [Cajanus cajan (L.) Millsp.] canned at different stages of maturation

    OpenAIRE

    1999-01-01

    O objetivo deste trabalho é viabilizar a introdução do guandu verde enlatado no mercado brasileiro. Foram realizadas três colheitas de grãos ainda verdes da cultivar IAC Fava Larga obtidos no 44º, 57º e 62º dia após a floração (DAF) e finalmente no 92º DAF, quando os grãos já possuíam cor pardo-amarelada. Para todos os lotes, após otimização das condições de enlatamento, 121ºC durante 5 a 6 minutos, efetuaram-se estudos quanto ao acúmulo de matéria seca, composição dos grãos, cor, textura e a...

  6. Coast cross (Cynodon dactylon (L. pers. hay and pigeon pea (Cajanus cajan (L. millsp digestibility and nutrients average intake by sheep under two feeding systems/ Consumo médio e digestibilidade do feno de capim “Coast cross” (Cynodon dactylon (L. pers. e feijão guandu (Cajanus cajan (L. Millsp em carneiros submetidos a dois regimes alimentares

    Directory of Open Access Journals (Sweden)

    Luiz Waldemar de Oliveira Souza

    2007-08-01

    Full Text Available This experiment was carried out with the objectives of determining the daily average intake (DAI, apparent digestibility coefficient (ADC of coast cross hay and pigeon pea nutrients and to evaluate thenitrogen balance in sheep. Ten wethers with average weight of 27.50 kg, alloted to suitable cages, were used. A completely randomized design with two treatments (T1= 80% coast cross hay + 20% pigeon peaand T2= 60% coast cross hay + 40% pigeon pea and five replicates, was used. The treatments were evaluated under two feeding systems (voluntary and restrict. Animals receiving ration of T2 presentedhigher DAI (g/kg PV 0.75 (PO experimento foi realizado com os objetivos de determinar o consumo médio diário (CMD, o coeficiente de digestibilidade aparente (CDA dos nutrientes do feno de capim Coast cross e feijão guandu e avaliar o balanço de nitrogênio (BN em ovinos. Foram utilizados dez ovinos machos, castrados, com peso médio de 27,50 kg, alojados em gaiolas para metabolismo, em delineamento inteiramente casualizado com dois tratamentos (T1 = 80% de feno de Coast cross + 20% de guandu e T2 = 60% de feno de Coast cross + 40% de guandu e cinco repetições por tratamento. Os tratamentos foram avaliados sob dois regimes alimentares (consumo voluntário e restrito. Os animais recebendo ração do T2 apresentaram maior CMD (g/kg PV0,75 (p < 0,05 de matéria seca (MS, proteína bruta (PB, fibra bruta (FB, matéria orgânica (MO e extrato não nitrogenado (ENN, do que aqueles animais recebendo ração do T1. O CDA da FB da ração T2 foi maior (p < 0,05 do que o da ração T1. O BN dos animais que receberam 40% de guandu (13,15g N/ dia foi superior aos daqueles que receberam 20% desta leguminosa (10,29g N/dia. Conclui-se que o feijão guandu pode ser utilizado como fonte protéica na alimentação de ruminantes, possibilitando CDA da MS próximo de 52% para animais em regime de consumo voluntário, e 81% para animais em regime de consumo restrito. O aumento na porcentagem de feijão guandu nas dietas de ovinos alimentados com feno de Coast-cross pode resultar em melhoria no CDA da FB da dieta, bem como no balanço de nitrogênio dos animais.

  7. Temperature stress in accumulation of free proline of pigeonpea seedlings from seeds treated with polyamines

    Directory of Open Access Journals (Sweden)

    Jéssica da Silva

    2015-02-01

    Full Text Available It was studied the effects of seed treatment with polyamines of pigeonpea for proline content of seedling in order to verify that the application of these polyamines attenuates temperature stress on germination and early seedling growth, and see if exogenous putrescine and spermidine induce the accumulation of proline, this amino acid may be biochemical and physiological indicator in seedlings that are under suboptimal temperatures and supraoptmail. The seeds of pigeonpea cv. BRS Mandarin and cv. Caqui, treated with a solution of 0.5 mM of putrescine and spermidine, were subjected to suboptimal temperature (20ºC, 18ºC, 16ºC and 14ºC and supraoptimal (36ºC, 38ºC, 40ºC and 44ºC for 24 hours and 48 hours. After these periods, were subjected to 25°C until day 10, when the percentage of seed germination were observed, the proline content of vegetative parts and the dry mass of the seedlings. A completely randomized design in a factorial arrangement was used 3x4x2+3 (solutions with polyamines, temperature stress by cooling/heating, time of exposure to stress, plus additional factors em25ºC with 0.0 mM and 0.5mM of Put and Spd, with four replicates of 25 seeds. The results were submitted to analysis of variance and means were compared by Tukey test at 5% probability, separately for each cultivar. Both exogenous polyamines attenuate the effects of cooling and heating, contributing to the growth of germinated seeds. Also, putrescine and spermidine exogenous mitigated the adverse effects by inducing proline accumulation, which leads to osmotic adjustment temperatures, although this physiological response has not minimized the negative effects of temperature stress on seedling growth of pigeonpea. Proline can be considered biochemical and physiological indicator in seedlings of both cultivars of pigeonpea treated with polyamines under temperature stress.

  8. Estimation of genetic variability in locally grown pulses (Cajans cajan (L.) Millsp and Vigna unguiculata (L.) Walp): a panacea for sourcing superior genotypes.

    Science.gov (United States)

    Udensi, O; Edu, E A; Umana, E J; Ikpeme, E V

    2011-03-15

    The negligence of breeders and farmers to explore and exploit landraces of pulses is worrisome and urgent measures needed to be set in motion to forestall major future crisis, taking into cognizance the high adaptability and nutritive values accredited to them. This study focused on the estimation of genetic variability and heritability of desirable morphological characters in Fiofio (Cajans cajan) and Olaudi and Akidi (Vigna unguiculata) with the aim of conservation. Three landraces of pulses were sown using randomized complete block design. The field experiment was carried out at the University of Calabar Experimental Farm, University of Calabar, Calabar, during 2008-2010 growing season. Phenotypic and genotypic variances and coefficients of variation and genetic advance were estimated on yield and yield-related traits. The results showed that there were considerable variations among the pulses for the traits studied. The result revealed high genetic variability in the number of leaf per plant, leaf area, number of flowers per plant, number of pods per plant and number of seeds per plant. It also showed that genetic variability in pod length and 100-seed weight was low. Heritability estimates obtained in the result were very high though the magnitude of genetic variability in the yield and yield-related traits was not proportional to the heritability estimates. The traits studied also show high genetic advance. These explicitly showed that there are sufficient genetic variations to warrant conservation and improvement in these extinction-threatened pulses studied.

  9. Phenotypic and molecular fingerprinting of fast growing rhizobia of field-grown pigeonpea from the eastern edge of the Brazilian Pantanal.

    Science.gov (United States)

    Costa, F M; Schiavo, J A; Brasil, M S; Leite, J; Xavier, G R; Fernandes, P I

    2014-01-21

    The aim of this study was to evaluate the diversity of rhizobial isolates obtained from root nodules of pigeonpea plants grown at the eastern edge of the Brazilian Pantanal. The bacterial isolates were isolated from root nodules from field-growing pigeonpea grown in two rural settlements of the Aquidauana municipality. The bacterial isolates were characterized phenotypically by means of cultural characterization, intrinsic antibiotic resistance (IAR), salt and high incubation temperature tolerance, and amylolytic and cellulolytic activities. The molecular characterization of the bacterial isolates was carried out using amplified ribosomal DNA restriction analysis (ARDRA) and Box-polymerase chain reaction (PCR) techniques. In addition, the symbiotic performance of selected rhizobial isolates was evaluated in a greenhouse experiment using sterile substrate. The phenotypic characterization revealed that the bacterial strains obtained from pigeonpea root nodules presented characteristics that are uncommon among rhizobial isolates, indicating the presence of new species nodulating the pigeonpea plants in the Brazilian Pantanal. The molecular fingerprinting of these bacterial isolates also showed a highly diverse collection, with both techniques revealing less than 25% similarity among bacterial isolates. The evaluation of symbiotic performance also indicated the presence of microorganisms with high potential to increase the growth and nitrogen content at the shoots of pigeonpea plants. The results obtained in this study indicate the presence of a highly diversified rhizobial community nodulating the pigeonpea at the eastern edge of the Brazilian Pantanal.

  10. Differential responses of photosynthetic parameters of pigeonpea and amaranth leaf discs to SO{sub 2}

    Energy Technology Data Exchange (ETDEWEB)

    Saraswathi, J.; Rao, K.V.M. [Andhra University, Visakhapatnam (India). Dept. of Botany

    1995-08-01

    The reduction in chlorophyll (Chl) and protein contents and the increase in amino acid content in leaf discs in response to aqueous SO{sub 2} exposure under continuous irradiance were more expressed in Amaranthus paniculatus (C-4 plant) than in Cajantus cajan (C-3 plant). The content of SH-compounds increased more in pigenonpea than in amaranth leaf discs in response to SO{sub 2}. Aqueous SO{sub 2} exposure also reduced the CO{sub 2} fixation and ribulose-1,5-bisphosphate carboxylase (RuBPC) and phosphoenolpyruvate carboxylase (PEPC) activities in leaf discs of both plant species. The differences in sensitivity of these plants to SO{sub 2} were related to their conversion efficiency of SO{sub 2} to less toxic substances and sulphydryl compounds.

  11. Dicty_cDB: Contig-U03348-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available QU... 38 0.12 2 ( FI228353 ) CccaBa045-E11TV CccABa Cajanus cajan genomic clon... 48 0.21 1 ( FI218856 ) Ccc...aBa046-J13TR CccABa Cajanus cajan genomic clon... 48 0.21 1 ( FI198911 ) CccaBa01...2-M12TR CccABa Cajanus cajan genomic clon... 48 0.21 1 ( FI198280 ) CccaBa008-J22TV CccABa Cajanus cajan gen

  12. Dicty_cDB: Contig-U05240-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available nomic DNA, chromoso... 44 0.42 2 ( FI202420 ) CccaBa022-F12TV CccABa Cajanus cajan genomic clon... 46 1.4 1 ...QUE... 44 5.5 1 ( FI227150 ) CccaBa043-H4TV CccABa Cajanus cajan genomic clone... 44 5.5 1 ( FI225933 ) Ccca...Ba055-O10TV CccABa Cajanus cajan genomic clon... 44 5.5 1 ( FI209274 ) CccaBa053-G1TR CccABa Cajanus cajan g

  13. Chemical composition, “in vitro” digestibility and production of woody forage legumes cultivated in sandy soils Composição bromatológica, digestibilidade "in vitro" e produção de biomassa de leguminosas forrageiras lenhosas cultivadas em solo arenoso

    National Research Council Canada - National Science Library

    Edimilson Volpe; Valdemir Antônio Laura; Maria Luiza Franceschi Nicodemo; Valéria Cristina Palmeira Zago; Tatiana da Costa Moreno Gama; Maria Graça Morais

    2009-01-01

    Considering the nutritional value and productivity of woody forage legumes Albizia lebbeck, Cajanus cajan, Cratylia argentea, Gliricidia sepium e Leucaena leucocephala, to be used in supplementation...

  14. Hypoglycemic of Cajanus scarabaeoides in glucose overloaded and streptozotocin-induced diabetic rats

    Directory of Open Access Journals (Sweden)

    Suman Pattanayak

    2009-06-01

    Full Text Available In light of traditional claim of Cajanus scarabaeoides (L in the treatment of diabetes, we studied the effects of different solvent extracts in normal, glucose over loaded normal and streptozotocin-induced diabetic rats. The methanolic extract (500 mg/kg orally was produce significantly reduce blood glucose level at normal, glucose over loaded normal rats, and streptozotocin-induced diabetic rats 15 days treatment whereas petroleum ether and chloroform extract (500 mg/kg orally did not exhibit any significant effect on three groups of rats. Histopathology studies on pancreas streptozotocin-induced diabetic rats inflammatory changes were detected in pancreatic islets results from selectively destroy of insulin producing β-cells. These changes are inhibited by C. scarabaeoides methanolic extract and gliclazide. The antidiabetic activity of methanolic extract may be due to the presence of flavonoids.

  15. Avaliação de algumas propriedades funcionais das farinhas de tremoço doce (Lupinus albus e feijão guandu (Cajanus cajan (L Millsp e sua utilização na produção de fiambre Evaluation of some functional properties of lupin sweet flour (Lupinus albus and faba bean flour (Cajanus cajan (L Millsp and their utilization in cooked ham production

    Directory of Open Access Journals (Sweden)

    Fernando Castilho

    2010-03-01

    Full Text Available O aumento na demanda de alimentos no mundo tem motivado pesquisadores a buscarem fontes alternativas a fim de enriquecer nutricionalmente alimentos industrializados e torná-los viáveis economicamente às populações menos favorecidas. O tremoço doce e o feijão guandu são leguminosas com elevado conteúdo proteico e grande potencial de utilização no enriquecimento nutricional de alimentos industrializados, além de desempenharem funcionalidades. Os resultados obtidos neste trabalho permitiram observar que a farinha de tremoço doce é capaz de absorver de 172 a 186% do seu peso em água e de ~62% em óleo, enquanto que a farinha de feijão guandu absorve cerca de 100% de seu peso em água e 50% em óleo. Boa atividade e estabilidade foram observadas para as emulsões obtidas com estas farinhas, indicando potencial de aplicação na produção de embutidos. Objetivou-se neste trabalho, avaliar algumas propriedades funcionais das farinhas de tremoço doce e feijão guandu e sua utilização na fabricação de fiambres. Os fiambres foram elaborados no Laboratório de Controle de Qualidade de Alimentos da Faculdade de Ciências Farmacêuticas da Universidade Estadual Paulista (UNESP, na cidade de Araraquara, com o intuito de oferecer um alimento enriquecido nutricionalmente. Quando comparados ao fiambre elaborado com a adição de concentrado proteico de soja, estes fiambres não apresentaram diferenças significativas nos atributos sensoriais aparência, cor, aroma, textura e impressão global, embora tivessem pouca aceitabilidade sensorial.The increase in food demand worldwide has motivated researchers to seek alternative food crops to nutritionally enrich industrialized foods and make them economically viable to poorer populations. Sweet lupin and faba bean are high protein content legumes of great potential for nutritional enrichment of industrialized foods, besides performing a functional purpose. The results obtained in this work enabled to identify that sweet lupin flour is able to absorb 172 to 186% of its weight in water, and ~62% in oil, while faba bean flour absorbs ~100% of its weight in water, and ~50% in oil. The emulsions obtained with both flours showed good activity and stability, suggesting a good application potential in the production of sausages. In the present work, some functional properties of the sweet lupin and faba bean flours were analyzed, including use in the production of cooked ham. The cooked ham was elaborated in the Quality Control Laboratory of foods in the Pharmaceutical Sciences Faculty in the University of São Paulo State (UNESP in Araraquara (SP, Brazil, offering an enriched food nutritionally. When compared to cooked hams prepared with the addition of soy protein, the experimental hams presented no significant sensorial differences, such as appearance, color, flavor, texture and overall impression, however they had had little sensorial acceptability.

  16. Dicty_cDB: Contig-U15353-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available P3AShA Triticum aestivu... 48 0.38 1 ( FI243267 ) CccaBb032-C18JR CccABb Cajanus cajan genomic clon... 34 1....1 2 ( FI234133 ) CccaBb023-N3JR CccABb Cajanus cajan genomic clone... 34 1.3 2 ( J02675 ) Rat prostatic sper...3.x1 NCI_CGAP_Pr35 Rattus norvegicus cDNA c... 46 1.5 1 ( FI247449 ) CccaBb026-C20JR CccABb Cajanus caja...n genomic clon... 34 1.7 2 ( FI261045 ) CccaBb058-E13JF CccABb Cajanus cajan genomic ...1-L20JR CccABb Cajanus cajan genomic clon... 34 2.0 2 ( FI248643 ) CccaBb026-F19JR CccABb Cajanus caja

  17. A Cyclin Dependent Kinase Regulatory Subunit (CKS) Gene of Pigeonpea Imparts Abiotic Stress Tolerance and Regulates Plant Growth and Development in Arabidopsis.

    Science.gov (United States)

    Tamirisa, Srinath; Vudem, Dashavantha R; Khareedu, Venkateswara R

    2017-01-01

    Frequent climatic changes in conjunction with other extreme environmental factors are known to affect growth, development and productivity of diverse crop plants. Pigeonpea, a major grain legume of the semiarid tropics, endowed with an excellent deep-root system, is known as one of the important drought tolerant crop plants. Cyclin dependent kinases (CDKs) are core cell cycle regulators and play important role in different aspects of plant growth and development. The cyclin-dependent kinase regulatory subunit gene (CKS) was isolated from the cDNA library of pigeonpea plants subjected to drought stress. Pigeonpea CKS (CcCKS) gene expression was detected in both the root and leaf tissues of pigeonpea and was upregulated by polyethylene glycol (PEG), mannitol, NaCl and abscisic acid (ABA) treatments. The overexpression of CcCKS gene in Arabidopsis significantly enhanced tolerance of transgenics to drought and salt stresses as evidenced by different physiological parameters. Under stress conditions, transgenics showed higher biomass, decreased rate of water loss, decreased MDA levels, higher free proline contents, and glutathione levels. Moreover, under stress conditions transgenics exhibited lower stomatal conductance, lower transpiration, and higher photosynthetic rates. However, under normal conditions, CcCKS-transgenics displayed decreased plant growth rate, increased cell size and decreased stomatal number compared to those of wild-type plants. Real-time polymerase chain reaction revealed that CcCKS could regulate the expression of both ABA-dependent and ABA-independent genes associated with abiotic stress tolerance as well as plant growth and development. As such, the CcCKS seems promising and might serve as a potential candidate gene for enhancing the abiotic stress tolerance of crop plants.

  18. Dicty_cDB: Contig-U02054-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available I250739 ) CccaBb021-K2JR CccABb Cajanus cajan genomic clone... 40 6.7 2 ( FE346698 ) CBIB6771.fwd CBIB_Daphn... CccaBb021-J12JR CccABb Cajanus cajan genomic clon... 40 8.0 2 ( CX205451 ) MNS09

  19. Dicty_cDB: Contig-U05106-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available Homo sapiens cDN... 34 0.83 2 ( FI237908 ) CccaBb014-E5JR CccABb Cajanus cajan genomic clone... 30 1.1 3 ( F...I266759 ) CccaBb064-K7JR CccABb Cajanus cajan genomic clone... 30 1.1 3 ( CS416754 ) Sequence 7 from Patent

  20. Dicty_cDB: Contig-U03236-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 46 3.1 1 ( FI239755 ) CccaBb014-F21JF CccABb Cajanus cajan genomic clon... 46 3....a064-N8TR CccABa Cajanus cajan genomic clone... 40 3.4 2 ( CU914721 ) Pig DNA sequence *** SEQUENCING IN PRO

  1. Dicty_cDB: Contig-U15652-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available C163421 ) Bos taurus clone CH240-141B7, *** SEQUENCING IN P... 46 2.6 1 ( FI223731 ) CccaBa058-P11TV CccABa Cajanus caja...n genomic clon... 46 2.6 1 ( FI221562 ) CccaBa035-P10TV CccABa Cajanus cajan genomic clon... 46

  2. Dicty_cDB: Contig-U02301-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available amoeba histolytica Sheared DNA Entam... 44 7.6 1 ( FI224294 ) CccaBa050-J3TV CccABa Cajanus caja...n genomic clone... 44 7.6 1 ( FI201490 ) CccaBa022-I9TV CccABa Cajanus cajan genomic clone...

  3. Dicty_cDB: Contig-U15195-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available clo... 44 6.4 1 ( FI256374 ) CccaBb038-N13JR CccABb Cajanus cajan genomic clon... 44 6.4 1 ( FI236314 ) Ccca...Bb030-O20JR CccABb Cajanus cajan genomic clon... 44 6.4 1 ( EJ230094 ) 1092402575041 Global-Ocean-Sampling_G

  4. Dicty_cDB: Contig-U04877-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 267210 ) CccaBb044-F6JR CccABb Cajanus cajan genomic clone... 44 3.4 1 ( FI261547... ) CccaBb058-D7JF CccABb Cajanus cajan genomic clone... 44 3.4 1 ( FH001788 ) CHO_OF276xk12f2.ab1 CHO_OF Nic

  5. Dicty_cDB: AFM729 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available stelium discoideum cDNA clone:ddc27c19, 3' ... 40 0.042 2 ( FI244407 ) CccaBb004-H23JF CccABb Cajanus caja...n genomic clon... 50 0.20 1 ( FI237621 ) CccaBb025-A5JR CccABb Cajanus cajan genomi

  6. Dicty_cDB: Contig-U04579-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 75 1 ( FI266530 ) CccaBb034-P16JR CccABb Cajanus cajan genomic clon... 46 0.75 1 ...( FI252525 ) CccaBb017-J13JR CccABb Cajanus cajan genomic clon... 46 0.75 1 ( FI072106 ) CHO_OF7325xd20f1.ab

  7. Dicty_cDB: Contig-U01737-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available embryo clone:m... 32 4.1 3 ( FI259718 ) CccaBb042-A12JF CccABb Cajanus cajan genomic clon... 36 5.0 2 ( AC1...... 38 9.4 2 ( FI248628 ) CccaBb026-D13JR CccABb Cajanus cajan genomic clon... 36

  8. Dicty_cDB: Contig-U05096-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available Microcebus murinus clone CH257-397E23, WORKING DR... 44 6.0 1 ( FI267105 ) CccaBb044-B7JR CccABb Cajanus caja...n genomic clone... 44 6.0 1 ( FI266561 ) CccaBb037-G2JF CccABb Cajanus cajan genomic clone... 44 6.0 1 ( C

  9. Dicty_cDB: Contig-U01201-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available .0 1 ( FI457626 ) CH322-28N20.x CHORI-322 Drosophila melanogaster g... 44 9.0 1 ( FI204272 ) CccaBa013-B4TR CccABa Cajanus caja...n genomic clone... 44 9.0 1 ( FI199123 ) CccaBa031-F5TR CccABa Cajanus cajan genomic clon

  10. Dicty_cDB: Contig-U01537-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available us laevis cDNA clone IMAGE:5514563, partial ... 46 2.6 1 ( FI249612 ) CccaBb031-I23JF CccABb Cajanus cajan g...enomic clon... 46 2.6 1 ( FI194717 ) CccaBa016-G17TR CccABa Cajanus cajan genomic clon... 46 2.6 1 ( EK14004

  11. Dicty_cDB: VFL417 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 17 ( FI217199 ) CccaBa060-L24TR CccABa Cajanus cajan genomic clon... 50 0.047 2 (... FI199692 ) CccaBa031-N10TR CccABa Cajanus cajan genomic clon... 50 0.047 2 ( EJ424712 ) 1093012257626 Globa

  12. Advances in genetics and molecular breeding of three legume crops of semi-arid tropics using next-generation sequencing and high-throughput genotyping technologies

    Indian Academy of Sciences (India)

    Rajeev K Varshney; Himabindu Kudapa; Manish Roorkiwal; Mahendar Thudi; Manish K Pandey; Rachit K Saxena; Siva K Chamarthi; Murali Mohan S; Nalini Mallikarjuna; Hari Upadhyaya; Pooran M Gaur; L Krishnamurthy; K B Saxena; Shyam N Nigam; Suresh Pande

    2012-11-01

    Molecular markers are the most powerful genomic tools to increase the efficiency and precision of breeding practices for crop improvement. Progress in the development of genomic resources in the leading legume crops of the semi-arid tropics (SAT), namely, chickpea (Cicer arietinum), pigeonpea (Cajanus cajan) and groundnut (Arachis hypogaea), as compared to other crop species like cereals, has been very slow. With the advances in next-generation sequencing (NGS) and high-throughput (HTP) genotyping methods, there is a shift in development of genomic resources including molecular markers in these crops. For instance, 2,000 to 3,000 novel simple sequence repeats (SSR) markers have been developed each for chickpea, pigeonpea and groundnut. Based on Sanger, 454/FLX and Illumina transcript reads, transcriptome assemblies have been developed for chickpea (44,845 transcript assembly contigs, or TACs) and pigeonpea (21,434 TACs). Illumina sequencing of some parental genotypes of mapping populations has resulted in the development of 120 million reads for chickpea and 128.9 million reads for pigeonpea. Alignment of these Illumina reads with respective transcriptome assemblies have provided > 10,000 SNPs each in chickpea and pigeonpea. A variety of SNP genotyping platforms including GoldenGate, VeraCode and Competitive Allele Specific PCR (KASPar) assays have been developed in chickpea and pigeonpea. By using above resources, the first-generation or comprehensive genetic maps have been developed in the three legume speciesmentioned above. Analysis of phenotyping data together with genotyping data has provided candidate markers for drought-tolerance-related root traits in chickpea, resistance to foliar diseases in groundnut and sterility mosaic disease (SMD) and fertility restoration in pigeonpea. Together with these trait-associated markers along with those already available, molecular breeding programmes have been initiated for enhancing drought tolerance, resistance to

  13. Advances in genetics and molecular breeding of three legume crops of semi-arid tropics using next-generation sequencing and high-throughput genotyping technologies.

    Science.gov (United States)

    Varshney, Rajeev K; Kudapa, Himabindu; Roorkiwal, Manish; Thudi, Mahendar; Pandey, Manish K; Saxena, Rachit K; Chamarthi, Siva K; Mohan, S Murali; Mallikarjuna, Nalini; Upadhyaya, Hari; Gaur, Pooran M; Krishnamurthy, L; Saxena, K B; Nigam, Shyam N; Pande, Suresh

    2012-11-01

    Molecular markers are the most powerful genomic tools to increase the efficiency and precision of breeding practices for crop improvement. Progress in the development of genomic resources in the leading legume crops of the semi-arid tropics (SAT), namely, chickpea (Cicer arietinum), pigeonpea (Cajanus cajan) and groundnut (Arachis hypogaea), as compared to other crop species like cereals, has been very slow. With the advances in next-generation sequencing (NGS) and high-throughput (HTP) genotyping methods, there is a shift in development of genomic resources including molecular markers in these crops. For instance, 2,000 to 3,000 novel simple sequence repeats (SSR) markers have been developed each for chickpea, pigeonpea and groundnut. Based on Sanger, 454/FLX and Illumina transcript reads, transcriptome assemblies have been developed for chickpea (44,845 transcript assembly contigs, or TACs) and pigeonpea (21,434 TACs). Illumina sequencing of some parental genotypes of mapping populations has resulted in the development of 120 million reads for chickpea and 128.9 million reads for pigeonpea. Alignment of these Illumina reads with respective transcriptome assemblies have provided more than 10,000 SNPs each in chickpea and pigeonpea. A variety of SNP genotyping platforms including GoldenGate, VeraCode and Competitive Allele Specific PCR (KASPar) assays have been developed in chickpea and pigeonpea. By using above resources, the first-generation or comprehensive genetic maps have been developed in the three legume speciesmentioned above. Analysis of phenotyping data together with genotyping data has provided candidate markers for drought-tolerance-related root traits in chickpea, resistance to foliar diseases in groundnut and sterility mosaic disease (SMD) and fertility restoration in pigeonpea. Together with these traitassociated markers along with those already available, molecular breeding programmes have been initiated for enhancing drought tolerance, resistance

  14. Dicty_cDB: Contig-U05002-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ... 46 1.2 1 ( FI271483 ) CccaBb048-K17JF CccABb Cajanus cajan genomic clon... 46 1.2 1 ( FI202347 ) CccaBa0...03-G11TV CccABa Cajanus cajan genomic clon... 46 1.2 1 ( FI200787 ) CccaBa030-K10TR CccABa Cajanus caja...piens genomic clo... 44 4.9 1 ( FI244612 ) CccaBb025-P9JF CccABb Cajanus cajan ge

  15. Dicty_cDB: Contig-U16195-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 16.TR Sheared DNA Trypanosoma bruc... 56 0.002 1 ( FI237470 ) CccaBb014-E21JF CccABb Cajanus caja... ( AY110562 ) Zea mays CL26590_1 mRNA sequence. 52 0.029 1 ( FI265286 ) CccaBb045-J21JF CccABb Cajanus cajan... genomic clon... 52 0.029 1 ( FI262825 ) CccaBb064-F23JF CccABb Cajanus cajan genomic clon... 52 0.029 1 ( F...I254950 ) CccaBb058-H16JR CccABb Cajanus cajan genomic clon... 52 0.029 1 ( ET713

  16. Dicty_cDB: Contig-U05042-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available a japonicum SJCHGC06716 protein mRNA, p... 48 0.20 1 ( FI266769 ) CccaBb064-M7JR CccABb Cajanus cajan genomi...c clone... 48 0.20 1 ( FI249869 ) CccaBb013-J14JR CccABb Cajanus cajan genomic clon... 48 0.20 1 ( FI234268 ...) CccaBb023-E13JF CccABb Cajanus cajan genomic clon... 48 0.20 1 ( FI203533 ) Ccc...aBa004-K10TV CccABa Cajanus cajan genomic clon... 48 0.20 1 ( AL976180 ) Danio rerio genomic clone DKEY-102J... 46 0.80 1 ( FI200335 ) CccaBa030-K13TR CccABa Cajanus cajan genomic clon... 46 0.80 1 ( FI114105 ) MUGQ_CH2

  17. Dicty_cDB: Contig-U01649-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available female placen... 42 3.5 2 ( FI227395 ) CccaBa053-C17TV CccABa Cajanus cajan genomic clon... 34 3.8 2 ( FI19...5606 ) CccaBa016-H5TR CccABa Cajanus cajan genomic clone... 34 4.4 2 ( FI269182 ) CccaBb062-A3JR CccABb Cajanus caja...V665505 ) CGN-3028 Pericarp Coffea canephora cDNA clone ccc... 42 4.8 2 ( FI230501 ) CccaBa060-F7TV CccABa Cajanus caja...5.4 2 ( FI239350 ) CccaBb010-J7JF CccABb Cajanus cajan genomic clone... 34 5.5 2 ...bal-Ocean-Sampling_GS-36-01-01-2... 40 5.9 2 ( FI229844 ) CccaBa061-L1TV CccABa Cajanus cajan genomic clone.

  18. A comparison of the variation in Indian populations of pigeonpea cyst nematode, Heterodera cajani revealed by morphometric and AFLP analysis

    Science.gov (United States)

    Rao, Sashi Bhushan; Rathi, Anamika; Gothalwal, Ragini; Atkinson, Howard; Rao, Uma

    2011-01-01

    Abstract The cyst nematode Heterodera cajani is one of the major endemic diseases of pigeonpea, an important legume for food security and protein nutrition in India. It occurs in several pulse crops grown over a range of Indian agro climatic conditions but the extent of its intraspecific variation is inadequately defined. In view of this, 11 populations of Heterodera cajani were analyzed using morphometrics and the results correlated with those obtained from an AFLP approach using 24 primer pair combinations that amplified a total of 1278 AFLP markers. The cluster solution from this binary data indicated similarities for five populations that differed from those suggested by morphometrics. The differences obtained could not be related to geographic distance between populations. The data suggests that recent and long distance dispersal has occurred whose causes need to be defined to restrict further field introductions. Four AFLP primer pairs clustered the populations similarly to that generated using all 24 primer pairs. This simplified approach may provide a rapid basis for discriminating populations for their future management and help to check further distribution in agricultural trade. It may also have potential to determine differences in populations that relate to host range or virulence to resistance genes. PMID:22259298

  19. Emaravirus: A Novel Genus of Multipartite, Negative Strand RNA Plant Viruses

    Directory of Open Access Journals (Sweden)

    Hans-Peter Mühlbach

    2012-09-01

    Full Text Available Ringspot symptoms in European mountain ash (Sorbus aucuparia L., fig mosaic, rose rosette, raspberry leaf blotch, pigeonpea sterility mosaic (Cajanus cajan and High Plains disease of maize and wheat were found to be associated with viruses that share several characteristics. They all have single-stranded multipartite RNA genomes of negative orientation. In some cases, double membrane-bound virus-like particles of 80 to 200 nm in diameter were found in infected tissue. Furthermore, at least five of these viruses were shown to be vectored by eriophyid mites. Sequences of European mountain ash ringspot-associated virus (EMARaV, Fig mosaic virus (FMV, rose rosette virus (RRV, raspberry leaf blotch virus (RLBV, pigeonpea sterility mosaic virus and High Plains virus strongly support their potential phylogenetic relationship. Therefore, after characterization of EMARaV, the novel genus Emaravirus was established, and FMV was the second virus species assigned to this genus. The recently sequenced RRV and RLBV are supposed to be additional members of this new group of plant RNA viruses.

  20. Exciting journey of 10 years from genomes to fields and markets: Some success stories of genomics-assisted breeding in chickpea, pigeonpea and groundnut.

    Science.gov (United States)

    Varshney, Rajeev K

    2016-01-01

    Legume crops such as chickpea, pigeonpea and groundnut, mostly grown in marginal environments, are the major source of nutrition and protein to the human population in Asia and Sub-Saharan Africa. These crops, however, have a low productivity, mainly due to their exposure to several biotic and abiotic stresses in the marginal environments. Until 2005, these crops had limited genomics resources and molecular breeding was very challenging. During the last decade (2005-2015), ICRISAT led demand-driven innovations in genome science and translated the massive genome information in breeding. For instance, large-scale genomic resources including draft genome assemblies, comprehensive genetic and physical maps, thousands of SSR markers, millions of SNPs, several high-throughput as well as low cost marker genotyping platforms have been developed in these crops. After mapping several breeding related traits, several success stories of translational genomics have become available in these legumes. These include development of superior lines with enhanced drought tolerance in chickpea, enhanced and pyramided resistance to Fusarium wilt and Ascochyta blight in chickpea, enhanced resistance to leaf rust in groundnut, improved oil quality in groundnut and utilization of markers for assessing purity of hybrids/parental lines in pigeonpea. Some of these stories together with future prospects have been discussed.

  1. Overexpression of pigeonpea stress-induced cold and drought regulatory gene (CcCDR) confers drought, salt, and cold tolerance in Arabidopsis.

    Science.gov (United States)

    Tamirisa, Srinath; Vudem, Dashavantha Reddy; Khareedu, Venkateswara Rao

    2014-09-01

    A potent cold and drought regulatory protein-encoding gene (CcCDR) was isolated from the subtractive cDNA library of pigeonpea plants subjected to drought stress. CcCDR was induced by different abiotic stress conditions in pigeonpea. Overexpression of CcCDR in Arabidopsis thaliana imparted enhanced tolerance against major abiotic stresses, namely drought, salinity, and low temperature, as evidenced by increased biomass, root length, and chlorophyll content. Transgenic plants also showed increased levels of antioxidant enzymes, proline, and reducing sugars under stress conditions. Furthermore, CcCDR-transgenic plants showed enhanced relative water content, osmotic potential, and cell membrane stability, as well as hypersensitivity to abscisic acid (ABA) as compared with control plants. Localization studies confirmed that CcCDR could enter the nucleus, as revealed by intense fluorescence, indicating its possible interaction with various nuclear proteins. Microarray analysis revealed that 1780 genes were up-regulated in CcCDR-transgenics compared with wild-type plants. Real-time PCR analysis on selected stress-responsive genes, involved in ABA-dependent and -independent signalling networks, revealed higher expression levels in transgenic plants, suggesting that CcCDR acts upstream of these genes. The overall results demonstrate the explicit role of CcCDR in conferring multiple abiotic stress tolerance at the whole-plant level. The multifunctional CcCDR seems promising as a prime candidate gene for enhancing abiotic stress tolerance in diverse plants.

  2. A bifunctional α-amylase/trypsin inhibitor from pigeonpea seeds: Purification, biochemical characterization and its bio-efficacy against Helicoverpa armigera.

    Science.gov (United States)

    Gadge, Prafull P; Wagh, Sandip K; Shaikh, Faiyaz K; Tak, Rajesh D; Padul, Manohar V; Kachole, Manvendra S

    2015-11-01

    This paper evaluates α-amylase inhibitor (α-AI) mediated defense of pigeonpea against Helicoverpa armigera. A bifunctional α-amylase/trypsin inhibitor was purified from the seeds of pigeonpea by native liquid phase isoelectric focusing (N-LP-IEF), affinity chromatography and preparative electrophoresis. Its in-vivo and in-vitro interaction with midgut amylases of H. armigera was studied along with growth inhibitory activity. One and two dimensional (2D) zymographic analyses revealed that the purified inhibitor is dimeric glycoprotein (60.2kDa and 56kDa) exist in a multi-isomeric form with five pI variants (pI 5.5 to 6.3). It was found to be heat labile with complete inactivation up to 80°C and stable over a wide range of pH (4-11). The slow binding and competitive type of α-amylase inhibition was observed with 0.08μM of dissociation constant (Ki) for the enzyme-inhibitor complex (EI). The internal protein sequence of two subunits obtained by mass spectrometry matched with cereal-type α-AI, a conserved domain from AAI_LTSS superfamily and sialyltransferase-like protein respectively. In-vivo studies indicated up-regulation of total midgut α-amylase activity with negative effect on growth rate of H. armigera suggesting its suitability for pest control.

  3. Dicty_cDB: Contig-U16005-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available b054-L5JR CccABb Cajanus cajan genomic clone... 32 5.0 2 ( AC132220 ) Homo sapiens chromosome 15, clone RP11...-1142D4, co... 38 5.0 6 ( FI232391 ) CccaBb002-M12JF CccABb Cajanus cajan genomic clon... 32 5.0 2 ( FI26811...2 ) CccaBb055-O23JR CccABb Cajanus cajan genomic clon... 32 5.0 2 ( FP090896 ) Pi

  4. Dicty_cDB: Contig-U00298-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ne R3-52G09, WOR... 46 0.97 1 ( FI263984 ) CccaBb039-L7JR CccABb Cajanus cajan genomic clone... 46 0.97 1 ( ... catenella mitochondrial cytb_1 gene f... 44 3.8 1 ( FI240547 ) CccaBb015-H8JR CccABb Cajanus caja...n genomic clone... 44 3.8 1 ( FI233294 ) CccaBb026-L11JF CccABb Cajanus cajan genomic clon.

  5. Dicty_cDB: Contig-U16249-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ... 38 8.4 2 ( FI231620 ) CccaBb001-K7JR CccABb Cajanus cajan genomic clone... 38... 8.5 2 ( AZ895509 ) RPCI-24-170F4.TJ RPCI-24 Mus musculus genomic clo... 36 9.6 2 ( FI237594 ) CccaBb016-K17JF CccABb Cajanus caja...n genomic clon... 38 9.6 2 ( FI273440 ) CccaBb050-E8JF CccABb Cajanus cajan genomic cl

  6. Dicty_cDB: Contig-U02161-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available .011 15 ( AE014828 ) Plasmodium falciparum 3D7 chromosome 14 section 1... 34 0.011 15 ( FI247919 ) CccaBb008-O16JF CccABb Cajanus caj...n Genomic Spe... 46 3.2 1 ( FI240260 ) CccaBb005-D5JF CccABb Cajanus cajan genomic clone... 46 3.2 1 ( FI232...251 ) CccaBb002-C15JF CccABb Cajanus cajan genomic clon... 46 3.2 1 ( FI203718 ) CccaBa026-A23TV CccABa Cajanus caja

  7. Management of disease complex caused by root knot nematode and root wilt fungus on pigeonpea through soil organically enriched with Vesicular Arbuscular Mycorrhiza, karanj (Pongamia pinnata) oilseed cake and farmyard manure.

    Science.gov (United States)

    Goswami, B K; Pandey, Rajesh Kumar; Goswami, Jaideep; Tewari, D D

    2007-11-01

    This investigation was undertaken to compare the percentage response of colonization and development of VA-Mycorrhiza (Glomus fasciculatum) on a number of pulse crops viz. cowpea, chickpea, soybean, pigeonpea and lentil under glasshouse conditions. Among the above-mentioned crops, pigeonpea exhibited the best performance and was selected for further studies. In this host the development and colonization percentage of G. fasciculatum was investigated under two separate substrates i. e. soil amended with FYM and karanj oilseed cake keeping a control treatment of field soil. A third treatment amended with karanj oilseed cake and farm yard manure (FYM) was also kept which responded best in terms of colonization percentage. This treatment showing improved plant health as well as integration with G. fasciculatum was selected as an ideal treatment for the management of disease complex caused by root knot nematode, Meloidogyne incognita and root wilt fungus, Fusarium udum on pigeonpea. Thus the treatment constituting FYM, karanj oilseed cake and VA-Mycorrhiza reduced the disease incidence caused by both maladies to a great extent with the most promising improvement in plant growth parameters as compared to all others. The present investigation, in addition to proposing an ideal eco-friendly treatment for the management of this disease complex also proposed an excellent medium for the proliferation of the obligate bio-protectant, G. fasciculatum.

  8. EFECTO DE LA GERMINACIÓN SOBRE EL CONTENIDO DE HIERRO Y CALCIO EN AMARANTO, QUINUA, GUANDUL Y SOYA EFEITO DA GERMINAÇÃO SOBRE CONTEUDO DA FERRO E CÁLCIO PRESENTES EM QUINOA, AMARANTO, GUANDU E SOJA GERMINATION EFFECT ON IRON AND CALCIUM CONTENT IN AMARANTH, QUINOA, PIGEON PEA AND SOYBEAN

    National Research Council Canada - National Science Library

    DIANA C CHAPARRO; REMIGIO Y PISMAG; ANA DE DIOS ELIZALDE C

    2011-01-01

    Se evaluaron los cambios en la cantidad de hierro y calcio durante el proceso germinativo en semillas de amaranto (Amaranthus sp), quinua (Chenopodium quinoa), guandul (Cajanus cajan) y soya (Glycine maxy...

  9. effect of pre-sowing hardening treatments using various plant growth ...

    African Journals Online (AJOL)

    DR. AMINU

    recently used to overcome the effect of abiotic stresses in .... Rate of imbibition was taken as change in fresh weight. ... Maximum weight change was however observed in seeds ..... stress resistance in Pigeon pea (Cajanus cajan L.)-II. Pre-.

  10. PROPRIEDADES DE EMULSÃO DA FARINHA E DO CONCENTRADO PROTÉICO DE FEIJÃO-GUANDU (Cajanus flavus DC. CULTIVAR FAVA-LARGA

    Directory of Open Access Journals (Sweden)

    José Paschoal BATISTUTI

    2009-07-01

    Full Text Available

    RESUMO: Com grãos decorticados de feijão-guandu (cajanus flavus DC. cultivar fava-larga, contendo 24,4% de proteína, foi preparado concentrado protéico, com rendimento de 17,6%. O concentrado protéico obtido apresentou conteúdo médio de 73,76% de proteína. Suspensões protéicas de concentrado protéico contendo 100 mg de proteína (N x 6,25 são capazes de emulsionar 35,7g de óleo. Emulsões obtidas da farinha de feijão-guandu, bem como de concentrado protéico, foram armazenadas em temperatura ambiente (25ºC e a 4°C, a fim de avaliar sua estabilidade. Foram avaliados, também, o efeito da concentração iônica, do pH, da diluição e do tratamento térmico na capacidade de emulsão do concentrado protéico de feijão-guandu decorticado. PALAVRAS – CHAVE: Feijão-guandu; Cajanus flavus DC.; farinha; concentrado protéico; propriedades de emulsão; estabilidade.

  11. Dicty_cDB: Contig-U01776-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ESS *** f... 46 0.69 1 ( FI254975 ) CccaBb058-L22JR CccABb Cajanus cajan genomic clon... 46 0.69 1 ( FI21687...3 ) CccaBa060-B9TR CccABa Cajanus cajan genomic clone... 46 0.69 1 ( ET965986 ) CHO_OF424xl19f1.ab1 CHO_OF N

  12. Dicty_cDB: Contig-U15230-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available WBb0099E05.f GM_WBb Glycine max genomic clone ... 34 0.19 2 ( FI258917 ) CccaBb053-C16JF CccABb Cajanus caja...... 36 0.35 2 ( FI273451 ) CccaBb050-G10JF CccABb Cajanus cajan genomic clon... 36 0.36 2 ( AM467491 ) Vitis

  13. Dicty_cDB: Contig-U04794-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 208071 ) CccaBa008-B18TR CccABa Cajanus cajan genomic clon... 46 1.1 1 ( EK307042 ) 1095462384214 Global-Oce...67 ) Homo sapiens chromosome 17 clone RP11-339D15 map ... 44 4.3 1 ( FI237139 ) CccaBb031-G17JR CccABb Cajanus caja

  14. Dicty_cDB: Contig-U15662-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available AZ349261 ) 1M0086M07F Mouse 10kb plasmid UUGC1M library Mus ... 46 6.8 1 ( FI215330 ) CccaBa054-C19TR CccABa Cajanus caja...n genomic clon... 46 6.8 1 ( FI208250 ) CccaBa006-H3TV CccABa Cajanus caja

  15. Dicty_cDB: Contig-U04937-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 1093041180643 Global-Ocean-Sampling_GS-28-01-01-1... 32 3.1 2 ( FI198726 ) CccaBa031-K14TR CccABa Cajanus caja...whole genome shotgu... 32 3.1 2 ( FI259820 ) CccaBb056-F5JF CccABb Cajanus cajan

  16. Dicty_cDB: Contig-U02803-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 34-H10JR CccABb Cajanus cajan genomic clon... 46 1.6 1 ( FI237436 ) CccaBb029-O11JF CccABb Cajanus caja...... 46 1.6 1 ( AC223898 ) Bos taurus clone CH240-455E16, WORKING DRAFT SEQU... 46 1.6 1 ( FI266492 ) CccaBb0

  17. Dicty_cDB: Contig-U02610-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 12-L20JR CccABb Cajanus cajan genomic clon... 52 0.028 1 ( FI238074 ) CccaBb015-C24JR CccABb Cajanus caja...z... 50 0.11 1 ( FI262698 ) CccaBb041-L13JR CccABb Cajanus cajan genomic clon... 50 0.11 1 ( CT419388 ) Sus ...52 0.028 1 ( BH126744 ) BARC-Satt578 Size-selected soybean genomic Glycin... 52 0.028 1 ( FI249972 ) CccaBb0

  18. Dicty_cDB: Contig-U01735-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available purpuratus clone R3-3047L05, W... 52 0.011 1 ( FI240593 ) CccaBb015-P24JR CccABb Cajanus caja...9 ) Gm_ISb001_018_B09R ISU Soybean BAC Library (pBelo... 44 2.8 1 ( FI251731 ) CccaBb028-F20JR CccABb Cajanus caja...n genomic clon... 44 2.8 1 ( FI235251 ) CccaBb024-K20JF CccABb Cajanus cajan genomic clon... 44 2.8 1

  19. Dicty_cDB: Contig-U03308-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 1 ( AC167093 ) Sorex araneus clone SA_Ba-207B12, WORKING DRAFT S... 48 0.32 1 ( FI239556 ) CccaBb022-P5JR CccABb Cajanus caja...3 1 ( AQ404889 ) HS_5044_B1_E02_SP6E RPCI-11 Human Male BAC Librar... 46 1.3 1 ( FI255788 ) CccaBb033-K4JR CccABb Cajanus caja...n genomic clone... 46 1.3 1 ( FI224926 ) CccaBa056-B8TV CccABa Cajanus cajan genomic clone

  20. Dicty_cDB: Contig-U05485-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 3 ( CZ514269 ) GMW2-40K24a.b1 GMW2 Glycine max genomic, genomic ... 36 0.081 2 ( FI243191 ) CccaBb009-E6JR CccABb Cajanus caja...n genomic clone... 40 0.082 2 ( FI267034 ) CccaBb063-C20JF CccABb Cajanus caja...nomic clo... 44 1.6 1 ( FI212785 ) CccaBa035-E23TR CccABa Cajanus cajan genomic clon... 44 1.6 1 ( FI040128

  1. Influence of green manure in physical and biological properties of soil and productivity in the culture of soybean

    Directory of Open Access Journals (Sweden)

    Ricardo Alves Cardoso

    2014-12-01

    Full Text Available Green manuring is the practice of using plant species in rotation, succession or intercropped with other crops, aiming improvement, maintenance and recovery of physical, chemical and biological soil properties. The objective was to evaluate the influence of different green manures on soil characteristics and productivity of soybean. The experiment was conducted in Maringá (PR in a randomized block design with six treatments and four replications: T1: oat (Avena Sativa, T2: black oat (Avena strigosa, T3: dwarf pigeon pea (Cajanus cajan, T4: radish (Raphanus sativus L., T5: white lupine (Lupinus albus and T6: control (fallow. At the end of the experiment, relations were established between the green manure used for soybean production, the production of biomass, the development of microorganisms and soil bulk density. The data were analyzed with statistical software and means were compared by Tukey test at 5% probability. The coverages provided higher content of dry matter were lupine, black oat and faba bean. Treatments that most influenced the increase of soil microorganisms were lupine, radish and pigeonpea. Regarding productivity, higher values were obtained in treatments with pigeon pea, lupine and oat. The apparent density of the soil, treatment with turnip showed better results.

  2. Dicty_cDB: Contig-U01704-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available .003 1 ( FI210818 ) CccaBa043-B3TR CccABa Cajanus cajan genomic clone... 42 0.003 3 ( AM462990 ) Vitis vinif....7 4 ( AM425165 ) Vitis vinifera contig VV78X176636.7, whole genome... 44 1.7 2 ( FI261308 ) CccaBb058-G10JF CccABb Cajanus caja...PROG... 38 2.1 5 ( FI272208 ) CccaBb036-K7JF CccABb Cajanus cajan genomic clone... 44 2.1 2 ( AC216609 ) Myo...bidopsis thali... 42 2.3 2 ( FI244820 ) CccaBb009-F19JF CccABb Cajanus cajan genomic clon... 44 2.3 2 ( BX93

  3. Attenuation of cadmium chloride induced cytotoxicity in murine hepatocytes by a protein isolated from the leaves of the herb Cajanus indicus L.

    Energy Technology Data Exchange (ETDEWEB)

    Sinha, Mahua; Manna, Prasenjit; Sil, Parames C. [Bose Institute, Department of Chemistry, Kolkata, West Bengal (India)

    2007-06-15

    Cadmium has been recognized as a strong environmental pollutant. Exposure to this heavy metal occurs through the intake of foodstuffs, drinking water and also via the inhalation of air. Present study was conducted to evaluate the protective effect of a 43 kDa protein, isolated from the leaves of the herb Cajanus indicus, against cadmium-induced cytotoxicity in hepatocytes. For this study, cadmium chloride (CdCl{sub 2}) has been used as the source of cadmium. Treatment of hepatocytes with 800 {mu}M CdCl{sub 2} for 3 h caused significant reduction in cell viability in association with the increased levels of glutamate pyruvate transaminase (GPT) and alkaline phosphatase (ALP) leakage. The activities of the antioxidant enzymes, superoxide dismutase, catalase (CAT), glutathione-S-transferase and glutathione reductase, and the levels of cellular metabolites, reduced glutathione (GSH) as well as total thiols have also been decreased under the same treatment. In addition, the toxin enhanced the levels of the lipid peroxidation end products and oxidized glutathione (GSSG). Incubation of hepatocytes with the protein at a dose of 0.1 mg/ml for 3 h prior to the toxin treatment (at a dose of 800 {mu}M for 3 h) restored the activities of all the antioxidant enzymes, the levels of GSH, total thiols, cell viability and also attenuated the increased levels of GPT, ALP, lipid peroxidation and GSSG. In addition, the protein resisted CdCl{sub 2} induced alterations of all the parameters when applied in combination with CdCl{sub 2}. Effects of a known antioxidant, vitamin E, and a non-relevant protein, bovine serum albumin against CdCl{sub 2} induced cytotoxicity have also been included in the study. Combining all, we would like to say that the protein possessed protective activity against CdCl{sub 2} induced cytotoxicity in mouse hepatocytes probably via its antioxidant property. (orig.)

  4. Study on the intercropping of Leucaena leucocephala with annual crops under dryland condition

    Energy Technology Data Exchange (ETDEWEB)

    Balasubramanian, T.N.; Robinson, J.G.; Ravikumar, V.

    1984-01-01

    Leucaena leucocephala was grown in paired rows with an interspace of 3 or 6 m between the two pairs of rows and was cut to a height of 50 or 100 cm. Sorghum, bajra (Pennisetum americanum) and red gram (Cajanus cajan) were grown either in the interspaces or in pure stands. Sorghum and bajra when grown both as pure stands and intercrops gave higher grain yields than seed yields of Cajanus cajan and the reduction in their yields as intercrops was also smaller. However, Leucaena leucocephala intercropped with Cajanus cajan in 6-m wide interspaces and cut to a height of 100 cm gave high fodder and seed yields and also produced more biomass in terms of fuel branch, roots and stump weight and gave the highest net returns. 5 references.

  5. Efeito de leguminosas nos atributos físicos e carbono orgânico de um Luvissolo Effect of legumes on the physical attributes and organic carbon of an Alfisol

    OpenAIRE

    João Tavares Nascimento; Ivandro de França da Silva; Roberval Diniz Santiago; Luiz de França da Silva Neto

    2005-01-01

    A degradação de um solo sob cultivo é resultante, principalmente, de seu manejo inadequado, com reflexos nas baixas produtividades das culturas. Com a finalidade de avaliar o efeito de leguminosas nos atributos físicos e carbono orgânico (CO) de um Luvissolo, realizou-se, no município de Alagoinha (PB), um experimento entre 1997 e 1999, com as seguintes espécies: crotalária (Crotalaria juncea, L), guandu (Cajanus cajan,L), guandu anão (Cajanus cajan (L) Millsp), calopogônio (Calopogonium mucu...

  6. Efeito de leguminosas nos atributos físicos e carbono orgânico de um Luvissolo

    OpenAIRE

    Nascimento,João Tavares; Silva,Ivandro de França da; Santiago,Roberval Diniz; Silva Neto,Luiz de França da

    2005-01-01

    A degradação de um solo sob cultivo é resultante, principalmente, de seu manejo inadequado, com reflexos nas baixas produtividades das culturas. Com a finalidade de avaliar o efeito de leguminosas nos atributos físicos e carbono orgânico (CO) de um Luvissolo, realizou-se, no município de Alagoinha (PB), um experimento entre 1997 e 1999, com as seguintes espécies: crotalária (Crotalaria juncea, L), guandu (Cajanus cajan,L), guandu anão (Cajanus cajan (L) Millsp), calopogônio (Calopogonium mucu...

  7. Dicty_cDB: Contig-U05668-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 166506 ) Bos taurus clone CH240-76C12, WORKING DRAFT SEQUE... 46 1.3 1 ( FI195395 ) CccaBa020-H20TR CccABa Cajanus caja...8_B1_D05_T7A CIT Approved Human Genomic Spe... 44 5.1 1 ( FI213479 ) CccaBa064-E15TR CccABa Cajanus cajan ge

  8. Caracterização de isolados de Rhizoctonia associados à queima foliar em Roraima = Characterization of Rhizoctonia isolates associated with foliar blight in Roraima.

    Directory of Open Access Journals (Sweden)

    Dayane Rodrigues Youssef

    2012-08-01

    Full Text Available O objetivo desse trabalho foi caracterizar isolados do fungo Rhizoctonia associados à queima foliar, obtidos de hospedeiros de importância econômica no estado de Roraima. Os isolados foram obtidos de plantas de feijão-caupi (Vigna unguiculata, soja (Glycine max, seringueira (Hevea brasiliensis, melancia (Citrullus lanatus, alface (Lactuca sativa e feijão-guandu (Cajanus cajan. Os parâmetros utilizados foram números de núcleos, grupo de anastomose e as características culturais da colônia, taxa de crescimento micelial e a formação de escleródios nos meios de cultura: batata dextrose agar (BDA, BDA+asparagina, BDA+extrato de levedura, Czapek Agar, maltose-peptona-agar, soil extract agar, sacarose-yeast-asparagina e V-8. Todos os 10 isolados estudados foram caracterizados como multinucleados e pertencentes à espécie Rhizoctonia solani. Três isolados de feijão-caupi, um de soja e o isolado de melancia foram identificados como AGI-1A e um isolado de feijãocaupi, um de soja e o isolado de feijão-guandu como AGI-1B. O isolado de seringueira não foi identificado como nenhum dos padrões de anastomose utilizado. Para a maioria dos isolados as maiores taxas de crescimento micelialforam obtidas no meio de cultura Soil Extract Agar. Dois tipos de escleródios, característicos do grupo AGI, foram observados: formação de 2-20 tufos placa-1 coloração variável, 1-2 mm e formação de 38-611 microescleródios placa-1, de coloração marrom, medindo 100 μm. A produção e o tipo de escleródio variaram com o isolado e o meio de cultura utilizado.The aim of this work was to characterize Rhizoctonia isolates associated with foliar blight symptom from hosts with economic importance at Roraima state. The isolates were recovered from cowpea (Vigna unguiculata, soybean (Glycine max, rubber tree (Hevea brasiliensis, watermelon (Citrullus lanatus, lettuce (Lactuca sativa and pigeonpea (Cajanus cajan. The evaluated characteristics were nuclear

  9. Dicty_cDB: Contig-U01815-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available nianus cD... 44 3.2 1 ( ES393277 ) MUS13-K08.y1d-s SHGC-MUS Mytilus californianus c...-L2JF CccABb Cajanus cajan genomic clone... 44 3.2 1 ( ES393488 ) MUS13-G14.y1d-s SHGC-MUS Mytilus califor

  10. Dicty_cDB: Contig-U15414-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 041-O2JF CccABb Cajanus cajan genomic clone... 44 8.3 1 ( ET015471 ) MUGQ_CH252P3... ... 44 8.3 1 ( AZ572658 ) 308PvE05 Pv MBN #30 Plasmodium vivax genomic 3', ... 44 8.3 1 ( FI254266 ) CccaBb

  11. Dicty_cDB: Contig-U02779-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ... 44 7.1 1 ( AC179286 ) Strongylocentrotus purpuratus clone R3-45I11, WOR... 44 7.1 1 ( FI194825 ) CccaBa0...05-N1TR CccABa Cajanus cajan genomic clone... 44 7.1 1 ( ET641970 ) JBVSE40TR JBVS Allium cepa genomic clone

  12. 491-IJBCS-Article-Dr Linus Nwaogu

    African Journals Online (AJOL)

    DR GATSING

    consumption of Cajanus cajan seed made pulses occasionally would not result to cyanide toxicity. ... major source of food for man due to their availability ... parts of Nigeria as pulses contain high ... conditions (27 °C and 12 hr light/dark cycle).

  13. Study on optimization of ultrasonic-assisted extraction technology and its antioxidant activity of pinostrobin from pigeonpea leaves%木豆叶球松素的超声提取工艺及其抗氧化活性研究

    Institute of Scientific and Technical Information of China (English)

    付丽楠; 袁肖寒; 顾成波; 蔡曼; 祖元刚

    2013-01-01

    在考察单因素(甲醇浓度、提取时间、提取温度和料液比)实验条件对木豆叶中球松素提取率影响的基础上,采用正交实验设计法优化得到球松素的最佳提取工艺条件,同时通过DPPH自由基清除实验评价了提取物的抗氧化活性.结果表明,木豆叶中球松素超声提取的最佳工艺条件为,甲醇提取浓度80%,提取时间60min,提取温度50℃,料液比1∶40(g/mL),球松素的提取率可达到1.832mg/g;甲醇提取物清除自由基的IC50值为0.281 mg/mL.本研究建立的木豆叶中球松素的超声提取工艺具有提取效率高、操作简便等优点,适合木豆叶中球松素的提取,且甲醇提取物具有良好的抗氧化活性.%Based on single-factor experimental results,effects of four parameters including methanol concentration,extraction time,extraction temperature and ratio of solid to liquid on the extraction yield of pinostrobin from pigeonpea leaves were optimized by orthogonal test design,and the antioxidant capacity of methanol extract (ME) was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging ability.Optimal extraction conditions were obtained as follows:methanol concentration 80%,extraction time 60min,extraction temperature 50℃ and solid-liquid ratio 1∶40(g/mL).Under optimum conditions,the extraction yield of pinostrobin reached 1.832mg/g,and ME of pigeonpea leaves exhibited good antioxidant activity with an IC50 value of 0.281mg/mL.Ultrasonic-assisted extraction technology established in the study possessed the advantages of high efficiency and ease of operation,which was suitable for the extraction of pinostrobin from pigeonpea leaves.

  14. Dinâmica do potássio nos resíduos vegetais de plantas de cobertura no Cerrado Potassium dynamics in crop residues of cover plants in Cerrado

    Directory of Open Access Journals (Sweden)

    José Luiz Rodrigues Torres

    2008-08-01

    Full Text Available A produção de biomassa, a manutenção dos resíduos vegetais sobre o solo e sua posterior decomposição são fatores de grande importância no estudo da ciclagem de nutrientes. Este estudo foi desenvolvido na área experimental do CEFET-Uberaba-MG, onde foram avaliados oito tipos de coberturas vegetais: milheto (Pennisetum americanum sin. tiphoydes, braquiária (Brachiaria brizantha, sorgo-forrageiro (Sorghum bicolor L. Moench, guandu (Cajanus cajan (L. Mill sp., crotalária (Crotalarea juncea, aveia-preta (Avena strigosa Schreb, pousio e área em preparo convencional de solo (testemunha em área de Cerrado, na região do Triângulo Mineiro. Avaliaram-se a fitomassa seca (FS, a decomposição dos resíduos em bolsas de decomposição, e a liberação de K. Utilizou-se um modelo matemático para descrever a decomposição dos resíduos e a liberação de K, e calcularam-se a constante de decomposição (k e o tempo de meia-vida (T½. O milheto, o sorgo e a crotalária foram as coberturas que apresentaram maiores produções de matéria seca. O maior acúmulo de K ocorreu em gramíneas e a maior liberação de K ocorreu no milheto, aveia, braquiária e crotalária nos primeiros 42 dias após manejo, nos dois períodos avaliados. A braquiária apresentou o menor T½ vida e a maior taxa de liberação de K.Crop residue production, plant residue maintenance and their decomposition are important factors in the understanding of nutrient recycling process. To evaluate K accumulation and release a study with eight cover crops types was developed: pearl millet (Pennisetum americanum sin. tiphoydes, brachiaria grass (Brachiaria brizantha, sorghum (Sorghum bicolor L. Moench, pigeonpea (Cajanus cajan (L. Millsp, sunn hemp (Crotalarea juncea and black oats (Avena strigosa Schreb, fallow land and conventional culture (control in the experimental area of CEFET-Uberaba-MG, in a Cerrado area. The dry mass production, crop residue decomposition in litter bags

  15. Supressão de plantas daninhas e produção de milho-verde orgânico em sistema de plantio direto Weed suppression and organic green corn production in no tillage system

    Directory of Open Access Journals (Sweden)

    L.R. Queiroz

    2010-06-01

    succession under an organic system. The trial was conducted with leguminous plants during the 2007/2008 season, at the Embrapa Corn and Sorghum Field Center in Sete Lagoas-MG. The experiment was arranged in a completely randomized block design with split-plots and four replications, with five species of leguminous plants being evaluated: jack bean (Canavalia ensiformes, pigeonpea (Cajanus cajan, mucuna-black (Mucuna aterrinum, mucuna dwarf (Mucuna deeringiana and Crotalaria juncea, plus a control, spontaneous vegetation of the area. On February/2008, the leguminous plants were cut close to soil and corn was planted over this mulch on March/06/2009. Weed samplings were collected at 15 and 30 days after corn emergence, which was randomly thrown on each plot framework, measuring 50 x 50 cm.. The weed plants within the framework were identified, weighed and counted. The results obtained showed that Mucuna aterrinum and Crotalaria juncea cover provided further weed biomass reduction during the two seasons evaluated. Higher commercial green corn ear yield was obtained when soil cover using Mucuna aterrinum and Crotalaria juncea straw was adopted.

  16. Avaliação de indicadores de qualidade do solo sob alternativas de recuperação do solo no Nordeste Paraense Evaluation of soil quality indicators under soil reclamation alternatives in Northeastern Pará State

    Directory of Open Access Journals (Sweden)

    Débora Veiga de Aragão

    2012-03-01

    , not yet sufficiently used, take into consideration the microbial activity that is more sensible to soil dynamics. This study was carried out in Marapanim-PA and using a completely randomized plot design, with four replications, to evaluate the behavior of some indicators such as microbial biomass, acid phosphatase, soil organic matter, organic carbon and available phosphorus - in a soil cultivated with cassava and corn, and in which the secondary vegetation was prepared by slash-and-trituration practice. The slash-and-trituration, rock phosphate and green manure factors were arranged in the following treatments: secondary vegetation; control; rock phosphate (RP; wonderbean Canavalia ensiformis (L. DC (W; RP + W; RP + pigeonpea Cajanus cajan (L. Millspaugh (P; RP + tithonia Tithonia diversifolia (Hemsley A. Gray (T; W + P; W + T. In four season of sampling, soil samples were taken from the 0 - 10 cm layer of each parcel to measure the indicator values. The indicators studied were influenced by the time of soil sampling. The acid phosphatase, microbial biomass nitrogen and microbial biomass carbon soil indicators were capable of detecting the effects of a higher number of soil reclamation treatments, while soil organic matter was the least capable. With the passing of time, the response of the indicators varied according to the factors studied.

  17. Chemical composition, “in vitro” digestibility and production of woody forage legumes cultivated in sandy soils Composição bromatológica, digestibilidade "in vitro" e produção de biomassa de leguminosas forrageiras lenhosas cultivadas em solo arenoso

    OpenAIRE

    Edimilson Volpe; Valdemir Antônio Laura; Maria Luiza Franceschi Nicodemo; Valéria Cristina Palmeira Zago; Tatiana da Costa Moreno Gama; Maria Graça Morais

    2009-01-01

    Considering the nutritional value and productivity of woody forage legumes Albizia lebbeck, Cajanus cajan, Cratylia argentea, Gliricidia sepium e Leucaena leucocephala, to be used in supplementation of cattle as a bank protein, five species in Quartzpisament were evaluated. The experiment was conducted from March 2006 to October 2007. The evaluations were carried out in two periods (rainy and dry), with two cuts by period. There was no difference in the accumulation of dry matter, in rainy pe...

  18. Produtividade do sorgo consorciado com feijão guandu e capim marandu em dois espaçamentos e composição bromatologica antes e após a ensilagem

    OpenAIRE

    Vicente, Elcio Ricardo José de Sousa

    2016-01-01

    O objetivo do projeto foi avaliar a produtividade e qualidade de silagem de sorgo (Sorghum bicolor (L.) Moench) solteiro e consorciado com Brachiaria brizantha (sin Urochloa brizantha) cv. Marandu; e feijão guandu (Cajanus cajan) cv. BRS Mandarin. O experimento foi desenvolvido na Fazenda Experimental da Universidade do Oeste Paulista - UNOESTE, localizada no município de Presidente Bernardes-SP, de dezembro de 2014 a maio de 2015. O delineamento experimental foi em faixas em esquema fatorial...

  19. Influence of nitrogen fertilization and green manure on the economic feasibility of no-tilled wheat in the Cerrado

    Directory of Open Access Journals (Sweden)

    Douglas de Castilho Gitti

    2012-04-01

    Full Text Available The search for higher profitability in wheat crop with cost reduction technologies that may promote sustainability is an important matter in Brazilian agriculture. This study evaluated the profitability of no-tilled wheat, reducing nitrogen topdressing doses with the cultivation of green manure before the wheat crop. The experiment was carried out in Selvíria (MS, Brazil, in 2009/10. The experiment was arranged in a randomized block design with 36 treatments in splitplots and four replicates. The plots were formed by six types of green manure: Cajanus cajan L. BRS Mandarin, Crotalaria juncea L., Pennisetum americanum L. BRS 1501, fallow area and mixed cropping of Pennisetum americanum L. + Cajanus cajan L. and Pennisetum americanum L. + crotalaria which provided straw for no-tilled wheat in the winter, following the rice crop in the summer. The subplots were formed by six levels of topdressing nitrogen (0, 25, 50, 75, 100 and 125 kg N ha-1 using urea as a nitrogen source. The wheat grown after green manure in the previous winter crop, with no nitrogen topdressing and a rate of 25 kg ha-1 N, had more frequently production costs above the gross income. Wheat production cost after the mixed cropping Pennisetum americanum L. + Cajanus cajan L. and Pennisetum americanum L. + Crotalaria juncea L. from the previous winter crop, combined with nitrogen rates of 50 and 75 kg N ha-1, provided better profitability compared with the other green manures evaluated.

  20. Estresse salino associado à aplicação exógena de espermidina no acúmulo de glicina betaína em Guandu Salt stress associated to exogenous spermidine application on the accumulation of glycine betaine in pigeonpea

    Directory of Open Access Journals (Sweden)

    Moacir Vinícius Pereira Destro

    2008-01-01

    Full Text Available O objetivo do presente trabalho foi verificar o efeito do estresse salino associado à aplicação exógena de espermidina (Spd nos genótipos de guandu, IAC Fava Larga e Caqui, no acúmulo de glicina betaína (GB, visando propor a sua utilização como marcador bioquímico-fisiológico à salinidade. O experimento foi instalado na Unesp, Faculdade de Ciências Agrárias e Veterinárias, em Jaboticabal (SP, no período de outubro a novembro de 2005, conduzido em sala de crescimento, com irradiância de 190 µmol m-2 s-1, fotoperíodo de 12 horas, temperatura de 25ºC e U.R. de 40%. A semeadura foi realizada em areia estéril, com adequado suprimento hídrico. Após 10 dias, as plântulas foram transferidas para solução nutritiva por 20 dias para aclimatação. A seguir, as plântulas foram alocadas nos tratamentos, sendo utilizado o delineamento experimental em blocos casualizados em esquema fatorial 2 x 2 x 4 com os fatores genótipos (IAC Fava Larga e Caqui, Spd (0,0 e 0,5 mM e estresse salino (0, 20, 60 e 80 mM de NaCl. Após 20 dias sob estresse, foram determinados os teores de GB. Pelos resultados obtidos, a GB pode ser indicada como um marcador bioquímico-fisiológico do estresse salino para o 'IAC Fava Larga'.This study was undertaken to evaluate the effect of salt stress associated to exogenous spermidine (Spd application on the accumulation of glycine betaine in the pigeonpea genotypes IAC Fava Larga and Caqui. The use of this compatible osmolyte as a biochemical-physiological indicator of salinity was also evaluated. The experiment was carried out at Unesp, Faculdade de Ciências Agrárias e Veterinárias, in Jaboticabal (SP, from October to November 2005, in a grow room with irradiance of 190 µmol m-2 s-1, photoperiod of 12 hours, temperature set to 25 ºC and the RH to 40%. Seeds were sowed in sterile sand with adequate water supply. After 10 days, seedlings were transferred to nutritive solution during 20 days for acclimation

  1. EFEITO DE ÉPOCAS DE SEMEADURA NA FISIOLOGIA E PRODUÇÃO DE FITOMASSA DE LEGUMINOSAS NOS CERRADOS DA REGIÃO DO MATOGROSSO DE GOIÁS

    Directory of Open Access Journals (Sweden)

    R.F. Amabile

    1996-05-01

    Full Text Available A adubação verde é uma prática conservacionista pouco utilizada nos Cerrados do Brasil Central. Visando obter informações sobre as características fenológicas das espécies de leguminosas utilizadas como adubos verdes, em diferentes épocas de semeadura na Região dos Cerrados, instalaram-se três experimentos, durante o ano agrícola de 1991/92, na área experimental do Centro Nacional de Pesquisa de Solos/Coordenadoria Regional Centro-Oeste - EMBRAPA, localizada na Estação Experimental da Empresa Goiana de Pesquisa Agropecuária - EMGOPA, em Senador Canedo, GO. As espécies testadas foram a Crotalaria juncea L., mucuna preta (Mucuna aterrima (Piper & Tracy Merr., guandu (Cajanus cajan (L. Millsp. e feijão-bravo do Ceará (Canavalia brasiliensis, em três épocas de semeadura. O delineamento experimental utilizado, dentro de cada época, foi de blocos ao acaso, apresentando três repetições. A C. juncea e o C. cajan apresentaram as maiores produções de fitomassa seca. O atraso da semeadura, em relação ao início da estação chuvosa, acelerou o florescimento das leguminosas e reduziu os rendimentos de fitomassa seca produzidos pela C. juncea e pelo C. cajan.The use of green manure is not a common conservation practice in "cerrado" regions. In order to investigate the phenological characteristics and mineral composition of leguminous plants used as green manure at different sowing times, three experiments were conducted during the 1991/92 growing seasons at the Soils National Research Center/West Center Coordination - EMBRAPA, in EMGOPA's Experimental Station, Senador Canedo, GO. Four species, Crotalaria juncea L., Mucuna aterrima (Piper & Tracy Merr., Cajanus cajan (L. Millsp. and Canavalia brasiliensis, were tested for three sowing dates. A randomized block design was used, with three replications. The results obtained allow the following conclusions: 1 In all sowing dates Crotalaria juncea e o Cajanus cajan showed the highest

  2. legumes in soil fertility management: the case of pigeonpea in ...

    African Journals Online (AJOL)

    High amounts leaf litter released by the crop in one season. (up to 3 t ha-') are ..... farmers, extension staff do not recommend intercropping. They ... becoming interested. Rotation. 3 asome crops do well when grown after others e.g maize.

  3. Genetic enhancement of pigeonpea for high latitude areas in ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-09-15

    Sep 15, 2009 ... from crossing combinations between five parental genotypes possessing marked differences in the genetic control of period to flowering and maturity as well as good ..... cropping systems in southern Africa and beyond.

  4. Genetic analysis of wild and cultivated germplasm of pigeonpea ...

    African Journals Online (AJOL)

    Ezedom Theresa

    Lens sp. (Sharma et al., 1995; Ahmad et al., 1996),. Phaseolus sp. (Beebe et al., 2000; Chiorato et al., 2007), ..... cultivated ICP 7543 and ICP 14144 revealed the highest ... Akkaya MS, Shoemaker RC, Specht JE, Bhagwat AA, Cregan PB.

  5. Environmental and agricultural benefits of a management system designed for sandy loam soils of the humid tropics Benefícios ambientais e agronômicos de um agrossistema definido para solos de textura franco arenosa do trópico úmido

    Directory of Open Access Journals (Sweden)

    Alana das Chagas Ferreira Aguiar

    2009-10-01

    Full Text Available A sustainable management of soils with low natural fertility on family farms in the humid tropics is a great challenge and overcoming it would be an enormous benefit for the environment and the farmers. The objective of this study was to assess the environmental and agronomic benefits of alley cropping, based on the evaluation of C sequestration, soil quality indicators, and corn yields. Combinations of four legumes were used in alley cropping systems in the following treatments: Clitoria fairchildiana + Cajanus cajan; Acacia mangium + Cajanus cajan; Leucaena leucocephala + Cajanus cajan; Clitoria fairchildiana + Leucaena leucocephala; Leucaena leucocephala + Acacia mangium and a control. Corn was used as a cash crop. The C content was determined in the different compartments of soil organic matter, CEC, available P, base saturation, percentage of water saturation, the period of the root hospitality factor below the critical level and corn yield. It was concluded that alley cropping could substitute the slash and burn system in the humid tropics. The main environmental benefit of alley cropping is the maintenance of a dynamic equilibrium between C input and output that could sustain up to 10 Mg ha-1 of C in the litter layer, decreasing atmospheric CO2 levels. Alley cropping is also beneficial from the agricultural point of view, because it increases base saturation and decreases physical resistance to root penetration in the soil layer 0 - 10 cm, which ensures the increase and sustainability of corn yield.O manejo sustentável dos solos de baixa fertilidade natural na agricultura familiar do trópico tem sido um grande desafio, que, se vencido, resultará em vantagens para o ambiente e para os agricultores. Este trabalho foi realizado com o objetivo de avaliar os benefícios ambiental e agronômico de um cultivo em aleias, por meio da determinação do C sequestrado, dos indicadores da qualidade do solo e da produtividade da cultura do milho

  6. Efficacy of nanostructured silica as a stored pulse protector against the infestation of bruchid beetle, Callosobruchus maculatus (Coleoptera: Bruchidae)

    Science.gov (United States)

    Arumugam, Ganesh; Velayutham, Veeramani; Shanmugavel, Sakthivelkumar; Sundaram, Janarthanan

    2016-03-01

    The treatment of hydrophobic silica nanoparticles (SNPs) with the pulse seeds of Cajanus cajan, Macrotyloma uniflorum, Vigna mungo, Vigna radiata, Cicer arietinum and Vigna unguiculata against the infestation of stored pulse beetle, Callosobruchus maculatus revealed a significant reduction in oviposition, adult emergence and seed damage potential. There was a complete retardation of growth of this beetle in the treated seeds of C. cajan. SNP-treated seeds of these six varieties of pulses revealed no effect on the growth of seeds as revealed by seed germination, growth rate of root and shoot. Similarly, the soil microflora measured in terms of colony forming units was not affected by silica nanoparticles upon its treatment with pulse seeds. The results of this study thus clearly demonstrated the useful nature of silica nanoparticles as seed protecting agent for the control of C. maculatus.

  7. Susceptibility of leguminous green manure species to Rhizoctonia solani and Sclerotium rolfsii

    Directory of Open Access Journals (Sweden)

    Trazilbo José de Paula Júnior

    2011-12-01

    Full Text Available We studied the susceptibility of species used as green manure in common bean fields to root rot (Rhizoctonia solani and southern blight (Sclerotium rolfsii. Seeds of Crotalaria breviflora, Canavalia ensiformis, Cajanus cajan, Dolichos lablab, Stizolobium cinereum, S. aterrimum, and the bean cvs. "Pérola", "Valente" and "Carnaval" were sown in soil infested by either R. solani AG-4 or S. rolfsii in greenhouse. The emergence of D. lablab seedlings in soil infested by R. solani dropped to 62%. C. breviflora, C. ensiformis and cv. "Valente" presented the lowest root rot severity. The pathogen S. rolfsii drastically reduced seedling emergence in all species; no C. cajan and S. cinereum seedling emerged. All plant species presented high southern blight severity. We conclude that leguminous crops are not suitable as green manure for areas of bean cultivation with high R. solani and S. rolfsii populations.

  8. Ecological Restoration Effect on Interplant of Tamarindus indica Artificial Forest in Yuanmou Dry and Hot Valley%元谋干热河谷罗望子人工林间作生态修复效应

    Institute of Scientific and Technical Information of China (English)

    李纪潮; 潘志贤; 岳学文; 何光熊; 史亮涛; 纪中华

    2014-01-01

    在建林15年的罗望子人工林内间作木豆(Cajanus cajan)、柱花草(Stylosanthes guianensias)、象草(Pennisetum purpureum)等灌草植物,构建新的生态修复模式.结果表明生态系统的物理性状、化学性状、经济性能均得到明显的提升.但豆科的木豆、柱花草效果更佳.

  9. Plant residues: short term effect on sulphate, borate, zinc and copper adsorption by an acid oxisol

    Directory of Open Access Journals (Sweden)

    Dias Ana Cristi Basile

    2003-01-01

    Full Text Available Laboratory experiments were carried out to examine the effects of plant residues on Cu, Zn, B and S adsorption by an acidic oxisol. The plant residues were: black oats (Avena strigosa, oil seed radish(Raphanus sativus, velvet beans (Stizolobium cinereum, and pigeon pea (Cajanus cajan collected at flowering stage. Plant residues increased Cu and Zn adsorptions and decreased B and S adsorptions. The results indicated that for short term effect plant residues decreased the availabilities of Cu and Zn through metal organic complex reactions and increased availabilities of S and B through competition with organic anions by the adsorption sites on soil.

  10. Avaliação quantitativa e qualitativa da fitomassa de leguminosas para uso como cobertura de solo

    Directory of Open Access Journals (Sweden)

    Nascimento João Tavares

    2004-01-01

    Full Text Available Para uso como adubo verde em um experimento em Alagoinha-PB, avaliaram-se doze espécies de leguminosas: crotalária (Crotalaria juncea L, guandu (Cajanus cajan L, guandu anão (Cajanus cajan (L Millsp, calopogônio (Calopogonium mucunoides L, feijão-de-porco (Canavalia ensiformis L, lab-lab (Dolichos lab lab L, kudzu tropical (Pueraria phaseoloides L, siratro (Macroptilium atropurpureum L, leucena (Leucaena leucocephala L, cunhã (Clitoria ternatea L, mucuna preta (Styzolobium aterrimum L, e mucuna cinza (Styzolobium cinereum Piper e Tracy, no DBC com 12 tratamentos, em parcelas de 20m2, com três repetições. Constataram-se as maiores produções de fitomassa para leucena, guandu, mucuna preta, kudzu tropical, feijão-de-porco e cunhã, enquanto que para a crotalária, a menor produção. A fitomassa do kudzu tropical mostrou-se com melhor qualidade para a incorporação.

  11. The first genetic map of pigeon pea based on diversity arrays technology (DArT) markers

    Indian Academy of Sciences (India)

    Shi Ying Yang; Rachit A. Saxena; Pawan L. Kulwal; Gavin J. Ash; Anuja Dubey; John D. I. Harper; Hari D. Upadhyaya; Ragini Gothalwal; Andrzej Kilian; Rajeev K. Varshney

    2011-04-01

    With an objective to develop a genetic map in pigeon pea (Cajanus spp.), a total of 554 diversity arrays technology (DArT) markers showed polymorphism in a pigeon pea F2 mapping population of 72 progenies derived from an interspecific cross of ICP 28 (Cajanus cajan) and ICPW 94 (Cajanus scarabaeoides). Approximately 13% of markers did not conform to expected segregation ratio. The total number of DArT marker loci segregating in Mendelian manner was 405 with 73.1% ($P \\gt 0.001$) of DArT markers having unique segregation patterns. Two groups of genetic maps were generated using DArT markers. While the maternal genetic linkage map had 122 unique DArT maternal marker loci, the paternal genetic linkage map has a total of 172 unique DArT paternal marker loci. The length of these two maps covered 270.0 cM and 451.6 cM, respectively. These are the first genetic linkage maps developed for pigeon pea, and this is the first report of genetic mapping in any grain legume using diversity arrays technology.

  12. Biological Nitrogen Fixation by Legumes and N Uptake by Coffee Plants

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    Eduardo de Sá Mendonça

    Full Text Available ABSTRACT Green manures are an alternative for substituting or supplementing mineral nitrogen fertilizers. The aim of this study was to quantify biological N fixation (BNF and the N contribution derived from BNF (N-BNF to N levels in leaves of coffee intercropped with legumes grown on four family farms located in the mountainous region of the Atlantic Forest Biome in the state of Minas Gerais, Brazil. The following green manures were evaluated: pinto peanuts (Arachis pintoi, calopo (Calopogonium mucunoides, crotalaria (Crotalaria spectabilis, Brazilian stylo (Stylosanthes guianensis, pigeon pea (Cajanus cajan, lablab beans (Dolichos lablab, and velvet beans (Stizolobium deeringianum, and spontaneous plants. The experimental design was randomized blocks with a 4 × 8 factorial arrangement (four agricultural properties and eight green manures, and four replications. One hundred grams of fresh matter of each green manure plant were dried in an oven to obtain the dry matter. We then performed chemical and biochemical characterizations and determined the levels of 15N and 14N, which were used to quantify BNF through the 15N (δ15N natural abundance technique. The legumes C. mucunoides, S. guianensis, C. cajan, and D. lablab had the highest rates of BNF, at 46.1, 45.9, 44.4, and 42.9 %, respectively. C. cajan was the legume that contributed the largest amount of N (44.42 kg ha-1 via BNF.C. cajan, C. spectabilis, and C. mucunoides transferred 55.8, 48.8, and 48.1 %, respectively, of the N from biological fixation to the coffee plants. The use of legumes intercropped with coffee plants is important in supplying N, as well as in transferring N derived from BNF to nutrition of the coffee plants.

  13. Evaluación de seis abonos verdes en un vertisol ústico en condiciones del Valle del Cauca

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    Bonilla C. C. R.

    2004-12-01

    Full Text Available En el Centro Experimental de la Universidad Nacional de Colombia-Sede Palmira, en un diseño de bloques completos al azar, se evaluaron seis tratamientos con tres repeticiones. (Cajanus cajan guandul arbóreo y arbustivo; Canavalia ensiformis, fríjol Canavalia; Cynodon niemfuensis, pasto estrella; Zea mays, Maíz y Cucúrbita moschata, zapallo. El pasto estrella y el zapallo fueron cultivos previos al ensayo; los otros materiales se sembraron por semilla a 50 cm en cuadro y se incorporaron al suelo a los 150 días cuando se cosechó el maíz (choclo. Maíz y canavalia aportaron mayor biomasa al suelo (10.068 y 9.748 kg/ha, respectivamente. C. cajan arbóreo y C. ensiformis aportaron las mayores cantidades de nitrógeno (254 y 213 kg/ha. El maíz y el pasto estrella, por sus altos contenidos de lignina, se descompusieron más lentamente. En casi todos los tratamientos se presentó incremento de la población microbiana, predominando la población de bacterias, seguida por hongos, fijadores de nitrógeno de vida libre y luego la población de actinomicetos. ABSTRACT Evaluation of six green manure materials in a Ustic Vertisol Soil of Cauca Valley, Colombia. This research was realized in the Experimental Center of the National University in Palmira. A design of a Randomized Completed Blocks, with six treatments and three repeticions was used. The treatments were two accesions of guandul (tree and shrub Cajanus cajan, Canavalia Canavalia ensiformis Corn Zea mays, Star grass Cynodon niemfuensis and Ramkin Cucúrbita moschata. The plot size was 20 m x 10 m. The materials were incorporated with tractor and disk plough when the maiz was colected as ear. The results showed that corn and Canavalia had the higher yields of fitomass (10068 and 9748 kg/ha, respectively. The legumes C. cajan (tree and C. ensiformis showed higher yileds of Nitrogen (254 and 213 kg/ha and biomolecules. Z. mays and C. nlemfuensis were the treatments with lower

  14. Impact of mine waste dumps on growth and biomass of economically important crops.

    Science.gov (United States)

    Mathiyazhagan, Narayanan; Natarajan, Devarajan

    2012-11-01

    The present study aimed to investigate the effect of magnesite and bauxite waste dumps on growth and biochemical parameters of some edible and economically important plants such as Vigna radiata, V. mungo, V. unguiculata, Eleusine coracana, Cajanus cajan, Pennisetum glaucum, Macrotyloma uniflorum, Oryza sativa, Sorghum bicolour, Sesamum indicum, Ricinus communis, Brassica juncea, Gossypium hirsutum and Jatropha curcas. The growth rate of all the crops was observed in the range of 75 to 100% in magnesite and 15 to 100% in bauxite mine soil. The moisture content of roots and shoots of all the crops were in the range of 24 to 77, 20 to 88% and 42 to 87, 59 to 88% respectively. The height of the crops was in the range of 2.6 to 48 cm in magnesite soil and 3 to 33 cm in bauxite soil. Thus the study shows that both mine soils reflects some physical and biomolecule impact on selected crops.

  15. Cover plants with potential use for crop-livestock integrated systems in the Cerrado region

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    Arminda Moreira de Carvalho

    2011-10-01

    Full Text Available The objective of this work was to evaluate the effects of lignin, hemicellulose, and cellulose concentrations in the decomposition process of cover plant residues with potential use in no-tillage with corn, for crop-livestock integrated system, in the Cerrado region. The experiment was carried out at Embrapa Cerrados, in Planaltina, DF, Brazil in a split plot experimental design. The plots were represented by the plant species and the subplots by harvesting times, with three replicates. The cover plants Urochloa ruziziensis, Canavalia brasiliensis, Cajanus cajan, Pennisetum glaucum, Mucuna aterrima, Raphanus sativus, Sorghum bicolor were evaluated together with spontaneous plants in the fallow. Cover plants with lower lignin concentrations and, consequently, higher residue decomposition such as C. brasiliensis and U. ruziziensis promoted higher corn yield. High concentrations of lignin inhibit plant residue decomposition and this is favorable for the soil cover. Lower concentrations of lignin result in accelerated plant decomposition, more efficient nutrient cycling, and higher corn yield.

  16. Composição química de grãos de feijao-guandu cultivar kaki

    OpenAIRE

    Teixeira,João Paulo Feijão; Spoladore,Dayse Soave; BRAGA,NELSON RAIMUNDO; Bulisani,Eduardo Antonio

    1985-01-01

    Foram analisadas sementes de tegumento creme e alaranjado de feijão-guandu (Cajanus cajan (L.) Milisp.), oriundas do cultivar Kaki, quanto aos teores de proteína, extrato etéreo, açúcares solúveis e polissacarídeos. Os dois tipos de sementes apresentaram composição química similar. Os teores médios encontrados foram: proteína 23,11%, extrato etéreo 1,34%, açúcar solúvel 9,46% e polissacarídeos 16,77%. A fração protéica apresentou baixos níveis de aminoácidos sulfurados, isoleucina, valina e t...

  17. Chemical Characteristics of Six Woody Species for Alley Cropping

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    Mosango, M.

    1999-01-01

    Full Text Available Leaves of six woody species (Leguminosae for alley cropping have been chemically analysed in order to evaluate their potentiality in the restoration of soil fertility. These species are : Acacia mangium, Cajanus cajan, Flemingia grahamiana, F. macrophylla, Leucaena leucocephala and Sesbania sesban. Nitrogen, carbon, cellulose, hemicellulose, lignin, active fraction and ash contents were determined as well as C/N and L/N ratios. AH these species appear to be rich in N and C. Fiber contents (cellulose, hemicellulose and lignin are globally low but variable from one species to another. C/N and L/N ratios are globally low. Among these species, Leucaena leucocephala and Senna spectabilis show the lowest C/N and LIN ratios. Such low values of C/N and L/N are normally found in species with rapid decomposition of organic matter.

  18. Sistema em fluxo para determinação espectrofotométrica de uréia em plasma de sangue animal empregando leguminosa como fonte natural da enzima urease

    Directory of Open Access Journals (Sweden)

    Luca Gilmara C.

    2001-01-01

    Full Text Available A spectrophotometric flow injection analysis (FIA procedure employing natural urease enzyme source for the determination of urea in animal blood plasma was developed. Among leguminous plants used in the Brazilian agriculture, the Cajanus cajan specie was selected as urease source considering its efficiency and availability. A minicolumn was filled with leguminous fragments and coupled to the FIA manifold, where urea was on-line converted to ammonium ions and subsequently it was quantified by spectrophotometry. The system was employed to determine urea in animal plasma samples without any prior treatment. Accuracy was assessed by comparison results with those obtained employing the official procedure and no significant difference at 90 % confidence level was observed. Other profitable features such as an analytical throughput of 30 determinations per hour, a reagent consumption of 19.2 mg sodium salicylate, 0.5 mg sodium hipochloride and a relative standard deviation of 1.4 % (n= 12 were also obtained.

  19. Antileishmanial and antifungal activity of plants used in traditional medicine in Brazil.

    Science.gov (United States)

    Braga, Fernanda G; Bouzada, Maria Lúcia M; Fabri, Rodrigo L; de O Matos, Magnum; Moreira, Francis O; Scio, Elita; Coimbra, Elaine S

    2007-05-04

    The antileishmanial and antifungal activity of 24 methanol extracts from 20 plants, all of them used in the Brazilian traditional medicine for the treatment of several infectious and inflammatory disorders, were evaluated against promastigotes forms of two species of Leishmania (L. amazonensis and L. chagasi) and two yeasts (Candida albicans and Cryptococcus neoformans). Among the 20 tested methanolic extracts, those of Vernonia polyanthes was the most active against L. amazonensis (IC(50) of 4 microg/ml), those of Ocimum gratissimum exhibited the best activity against L. chagasi (IC(50) of 71 microg/ml). Concerning antifungical activity, Schinus terebintifolius, O. gratissimum, Cajanus cajan, and Piper aduncum extracts were the most active against C. albicans (MIC of 1.25 mg/ml) whereas Bixa orellana, O. gratissimum and Syzygium cumini exhibited the best activity against C. neoformans (MIC of 0.078 mg/ml).

  20. Os sistemas agroflorestais como alternativa econômica em pequenas propriedades rurais: estudo de caso

    Directory of Open Access Journals (Sweden)

    Mário Jorge Santos Campos dos

    2002-01-01

    Full Text Available The Agroforestry Systems have been considered as an alternative approach for the stability of the ecosystems and in the same time promote the efficiency and optimization of the natural resources in the production in an integrated form. The objective of this case study is the economic evaluation of agroforestry systems in the Pontal do Paranapanema region. The Agroforestry System being studied is formed by agricultural species: corn (Zea maiz, guandu bean (Cajanus cajan, carioquinha, (Vigna unguiculata, black (Phaseolus vulgaris and cassava (Manihot esculenta with forestry species: Eucalyptus citriodora, Eucalyptus camaldulensis. The economic evaluation was based on economical criteria such as Net Present Value (NPL and Cost Benefict (C/B for the annual agricultural culture, Land Expectation Value (LET for the agroforestry component. The results showed that the agroforestry systems are economically acceptable to small farmers working in the same conditions presented in this study.

  1. A PRELIMINARY STUDY ON FERMENTATION OF CANAVALIA ENSIFORMIS SEEDS

    Directory of Open Access Journals (Sweden)

    Indrawati Gandjar

    2012-09-01

    Full Text Available Pada umumnya di Indonesia kacang kedele difermentasi menjadi tempe kedele. Hasil fermentasi ini merupakan sumber protein dan vitamin yang penting dalam makanan penduduk sehari-hari. Di daerah-daerah tertentu di pulau Jawa beberapa jenis kacang-kacangan seperti Koro benguk (Mucuna pruriens, koro pedang (Canavalia ensiformis dan kacang hiris (Cajanus cajan juga difermentasi menjadi semacam tempe kedele. Dalam penelitian ini telah dilakukan fermentasi biji C. ensiformis menjadi tempe. Sejumlah kapang Rhizopus telah diperiksa kemampuannya untuk memfermentasikan biji-biji tersebut. R.oryzae R128 mampu menghasilkan tempe koro pedang dalam waktu 26 jam inkubasi pada suhu 30°C. Telah diteliti beberapa perubahan biokimia yang terjadi dalam substrat. Selama proses fermentasi berlangsung terjadi peningkatan dari kadar senyawa asam total, nitrogen amino dan karbohidrat terlarut. Analisa zat gizi dalam biji C. ensiformis mentah dan sesudah difermentasi menjadi tempe telah dilaku­kan.

  2. Dicty_cDB: Contig-U01765-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available G-aaa96e04.b1 Sand_fly_EST_Normalized Phleboto... 36 0.68 2 ( FK811594 ) PRAG-aac82g02.g1 Sand_fly_EST_Normalized Phleboto......borelli mitochondrion cytochrome oxid... 40 1.8 2 ( AC192729 ) Pan troglodytes BAC clone CH251-503N9 from chromo...... ) Human DNA sequence from clone RP11-176N14 on chro... 44 3.7 1 ( AL160192 ) Human chromosome 14 DNA sequen... Cajanus cajan genomic clone... 44 3.7 1 ( ER982879 ) MUGQ_CH252P307E11T7_CP496_044 CHORI-252 Vervet Mo... 4...01-1... 44 3.7 1 ( EI396940 ) MUGQ_CH252P211J14T7_CN333_055 CHORI-252 Vervet Mo... 44 3.7 1 ( ED385644 ) AUA

  3. Macrofauna edáfica associada a plantas de cobertura em plantio direto em um Latossolo Vermelho do Cerrado Soil macrofauna communities and cover crops in a Cerrado Oxisol under no tillage

    Directory of Open Access Journals (Sweden)

    Glenio Guimarães Santos

    2008-01-01

    Full Text Available O objetivo deste trabalho foi caracterizar a macrofauna edáfica e avaliar o efeito de plantas de cobertura em plantio direto, nos principais grupos da macrofauna do solo, em duas épocas de avaliação em um Latossolo Vermelho distroférrico. O delineamento experimental foi o de blocos ao acaso, com oito tratamentos (plantas de cobertura e quatro repetições. As plantas de cobertura: Crotalaria juncea, guandu-anão (Cajanus cajan, Stylosanthes guianensis, Brachiaria brizantha, B. brizantha consorciada com milho (Zea mays, milheto (Pennisetum glaucum, mombaça (Panicum maximum e Sorghum bicolor foram cultivadas de novembro a abril. Em setembro de cada ano, foi realizado o plantio de feijão, em cultivo irrigado por pivô central. A área útil em cada parcela foi de 60 m². Amostras de solo na forma de monólitos (25x25 cm foram retiradas aleatoriamente em cada parcela, para contagem da macrofauna, às profundidades de 0-10 cm e 10-20 cm, em abril e em setembro de 2005. Os grupos taxonômicos, identificados em ordem decrescente de densidade relativa, são: Formicidae, Oligochaeta, Dermaptera, Coleoptera, Hemiptera, Miriapoda, Isoptera, Araneae, Lepidoptera, Blattodea e larvas de Diptera. Crotalaria juncea apresentou maior densidade de macrofauna, seguida por B. Brizantha, B. Brizantha consorciada com milho, Sorghum bicolor, Stylosanthes guianensis, Cajanus Cajans, Pennisetum Glaucum e Panicum maximum. O uso das plantas de cobertura, associado à irrigação na avaliação de setembro, favorece a colonização do solo pela macrofauna.The objective of this work was to characterize soil fauna groups and to evaluate the effects of cover crops under no-tillage system, in a Cerrado Oxisol, in two evaluation periods. The cover crops: Crotalaria juncea, Cajanus cajan, Stylosanthes guianensis, Brachiaria brizantha, Brachiaria brizantha/ Zea mays association, Pennisetum glaucum, Panicum maximum and Sorghum bicolor were cultivated from November to April

  4. Efeito de leguminosas nos atributos físicos e carbono orgânico de um Luvissolo Effect of legumes on the physical attributes and organic carbon of an Alfisol

    Directory of Open Access Journals (Sweden)

    João Tavares Nascimento

    2005-10-01

    Full Text Available A degradação de um solo sob cultivo é resultante, principalmente, de seu manejo inadequado, com reflexos nas baixas produtividades das culturas. Com a finalidade de avaliar o efeito de leguminosas nos atributos físicos e carbono orgânico (CO de um Luvissolo, realizou-se, no município de Alagoinha (PB, um experimento entre 1997 e 1999, com as seguintes espécies: crotalária (Crotalaria juncea, L, guandu (Cajanus cajan,L, guandu anão (Cajanus cajan (L Millsp, calopogônio (Calopogonium mucunoides, L, feijão-de-porco (Canavalia ensiforme, L, lab-lab (Dolichos lab lab, L, kudzu tropical (Pueraria phaseoloides, L, siratro (Macroptilium atropurpureum, L, leucena (Leucaena leucocephala, L, cunhã (Clitoria ternatea, L, mucuna preta (Styzolobium aterrimum, L, mucuna cinza (Styzolobium cinereum Piper e Tracy, e uma parcela com vegetação espontânea, como testemunha. O delineamento experimental utilizado foi o de blocos ao acaso em esquema de parcela subdividida, com distribuição de treze tratamentos principais na parcela (12 leguminosas + 1 testemunha e três tratamentos secundários, representados pelas profundidades de coleta de amostras de solo (0,00-0,10; 0,10-0,20; e 0,20-0,30 m, na subparcela, com três repetições. Na condição edafoclimática deste experimento, durante os três anos de trabalho, constatou-se que o emprego das leguminosas em relação à testemunha manteve inalterados o CO do solo, a densidade do solo, a porosidade total, a argila dispersa em água e a estabilidade dos agregados do solo.Soil degradation under cultivation results mainly from the inadequate management and it reflects in low crop yields. With the purpose of evaluating the effect of legumes on physical attributes and organic carbon content of an Alfisol, an experiment was conducted in the Alagoinha County-PB, Brazil, between 1997 and 1999, with the following species: Crotalaria juncea L, Cajanus cajan L, Cajanus cajan (L Millsp, Calopogonium mucunoides

  5. Tolerancia de cinco leguminosas al aluminio en solución nutritiva.

    Directory of Open Access Journals (Sweden)

    Pérez N Juan Carlos

    2003-06-01

    Full Text Available Se realizó una investigación para determinar el grado de tolerancia a aluminio (Al de cinco leguminosas tropicales: guandul (Cajanus cajan L. Mills, canavalia (Canavalia ensiformis L., frijol jacinto (Dolichos lablab L., leucaena (Leucaena leucocephala (C. Lam. De Witt y fríjol terciopelo (Stizolobium deeringianum Bort.. Las plantas crecieron bajo cinco concentraciones de Al: 0, 2, 4, 8 y 16 mg L-1 en solución nutritiva. La leguminosa más afectada por las concentraciones de Al fue L. leucocephala, la cual exhibió una disminución significativa en la producción de materia seca y en la traslocación de P hacia la parte aérea. En las otras leguminosas la materia seca y la distribución de P fueron poco afectados.

  6. Selection of species tolerant to the herbicide sulfentrazone with potential for phytoremediation of contaminated soilsSeleção de espécies tolerantes ao herbicida sulfentrazone com potencial para a fitorremediação de solos contaminados

    Directory of Open Access Journals (Sweden)

    João Carlos Madalão

    Full Text Available This paper aimed to select species with potential for phytoremediation of soils contaminated with the herbicide sulfentrazone. Eight species were evaluated: Arachis pintoi, Eleusine coracana, Crotalaria spectabilis, Crotalaria ochroleuca, Cajanus cajan, Leucaena leucocephala, Stizolobium cinereum, and Raphanus sativus. The experiment was set-up inside a greenhouse, using pots with a capacity of 6dm3 filled with soil samples collected at a depth of 0-20cm. The experimental design was arranged into randomised blocks in a factorial scheme (8 × 5 with four replications, which consisted of the combination between the species and five doses of sulfentrazone (0, 200, 400, 800, and 1,600g ha-1. The herbicide phytotoxicity, plant heights, and dry masses of shoots and roots were evaluated. The species Cajanus cajan and Leucaena leucocephala had a higher tolerance to sulfentrazone up to a dose of 400g ha-1, showing minor symptoms of phytotoxicity and smaller decreases in plant heights and in dry matter accumulation, both in the shoots and roots, when compared to the control treatment, indicating, thus, the potential to be used for further studies on phytoremediation of sulfentrazone in soil. Este trabalho teve como objetivo selecionar espécies com potencial para a fitorremediação de solos contaminados com o herbicida sulfentrazone. Foram avaliadas oito espécies: Arachis pintoi, Eleusine coracana, Crotalaria spectabilis, Crotalaria ochroleuca, Cajanus cajan, Leucaena leucocephala, Stizolobium cinereum e Raphanus sativus. O experimento foi instalado em casa de vegetação, com a utilização de vasos com capacidade para 6 dm3 preenchidos com porções de solo coletadas na profundidade de 0-20 cm. O delineamento experimental foi o de blocos casualizados em esquema fatorial 8 × 5, com quatro repetições, composto pela combinação entre as espécies e cinco doses do sulfentrazone (0, 200, 400, 800 e 1.600 g ha-1. Foram avaliadas a fitotoxicidade do

  7. Adubação verde e alterações nas características químicas de um Cambissolo na região de Ji-Paraná-RO Green manure and changes on chemical characteristics of a soil in the Ji-Paraná-RO region

    Directory of Open Access Journals (Sweden)

    Elaine Almeida Delarmelinda

    2010-09-01

    Full Text Available A adubação verde promove benefícios nas características químicas, físicas e biológicas do solo. O trabalho avaliou o efeito da incorporação de diferentes adubos verdes nas características químicas de um Cambissolo háplico eutrófico. Foi utilizado o delineamento em blocos ao acaso, com oito tratamentos e três repetições. Os tratamentos consistiram das seguintes leguminosas: Calopogonium mucunoides, Crotalaria juncea, C. spectabilis, Cajanus cajan, Macrotyloma, Mucuna pruriens, Pueraria phaseoloides e a testemunha (sem leguminosa. As avaliações foram realizadas 60 dias após o corte e incorporação. A utilização de leguminosas como adubação verde proporcionou aumento nos teores de matéria orgânica, soma de bases e percentagem de saturação por bases, destacando-se a Pueraria phaseoloides, C. juncea e C. spectabilis.The green manure promotes benefits on the chemical, physical and biological properties of soil. The experiment evaluated the effect of incorporation of differents green manure on the chemistry characteristics of a soil. Experimental design used was a randomized block, with eight treatments and three replications. Treatments were: Calopogonium mucunoides, Crotalaria juncea, Crotalaria spectabilis, Cajanus cajan, Macrotyloma axillare, Mucuna pruriens and Pueraria phaseoloides and the treatment control (without leguminous. Evaluations of soil samplings were performed in 60 days after handling were performed. The results showed significant effects of the leguminous crop Pueraria phaseoloides, C. juncea and C. spectabilis on the fertility of the soil, with significant increments of organic matter, exchangeable bases and in the base saturation.

  8. Degradabilidade ruminal da matéria seca e da proteína bruta de folhas e folíolos de forrageiras tropicais Ruminal degradabilities of dry matter and crude protein of tropical forages

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    Cristina Mattos Veloso

    2006-04-01

    Full Text Available Foram determinadas as degradabilidades in situ da MS e PB dos folíolos de três leguminosas tropicais leucena (Leucaena leucocephala (Lam. de Wit, guandu (Cajanus cajan (L. Millsp. e soja perene (Neonotonia wightii e de folhas de outras duas forrageiras tropicais rami (Boehmeria nivea (L. Gaudich e mandioca (Manihot esculenta Crantz. Amostras de 3 g (guandu, soja e rami ou 5 g (leucena e mandioca do material foram incubadas no rúmen de quatro novilhos por períodos de 3, 6, 12, 18, 24 e 48 horas. As degradabilidades efetivas da MS e da PB para a taxa de passagem de 5% hora foram elevadas (acima de 60%. As taxas de degradação, exceto a do guandu, também foram consideradas elevadas. As forrageiras apresentaram alta digestibilidade total da proteína (acima de 88%, exceto os folíolos de guandu (69%. O guandu foi a forrageira com menor potencial de degradação da proteína.In situ DM and CP degradabilities of leaflets of three tropical legumes: leucaena (Leucaena leucocephala (Lam. de Wit, pigeon pea (Cajanus cajan (L. Millsp., and glycine (Neonotonia wightii and of leaves of two other tropical forages: ramie (Boehmeria nivea (L. Gaudich and cassava (Manihot esculenta Crantz were determined in this trial. Three grams of pigeon pea, glycine, and ramie and 5 g of leucaena and cassava were incubated in the rumen of four steers for 3, 6, 12, 18, 24, and 48 hours. Effective degradabilities of DM and CP assuming a passage rate of 5%/hour were greater than 60%. Degradation rates, except for pigeon pea, were also high in this study. Forages had high total digestibility of protein (greater than 88%, except for pigeon pea leaflets that was 69%. Pigeon pea was the forage with the lowest potential for ruminal protein degradation.

  9. Occurence and host specificity of indigenous rhizobia from soils of São Paulo State, Brazil Ocorrência de rizóbios nativos em plantas hospedeiras de solos nativos do Estado de São Paulo, Brasil

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    Maria Luiza Colognesi de Oliveira Lombardi

    2009-08-01

    Full Text Available The occurrence of rhizobial communities at four sites under natural vegetation and one site under pasture were examined. Isolates of rhizobia originating from crotalaria (C. junceae, common bean (Phaseolus vulgaris and pigeon pea (Cajanus cajan were studied in relation to population density, host specificity and the interaction between rhizobial occurrence, climatic conditions and soil properties. pH values and potential acidity were the soil properties that most affected rhizobial occurrence. Rhizobia from crotalaria and common bean were evaluated at four sites, and from pigeon pea, at five sites. Common bean was the most specific legume, followed by peanuts, crotalaria and pigeon pea.Foi examinada a ocorrência de comunidades de rizóbios em quatro locais de vegetação natural e um local de pastagem. Isolados de rizóbio originados de crotalária (C. junceae, feijão (Phaseolus vulgaris e guandu (Cajanus cajan foram estudados em relação à densidade populacional, planta hospedeira e interação entre ocorrência de rizóbio, condições climáticas e propriedades do solo. Os valores de pH e potencial de acidez foram as propriedades do solo que mais contribuíram para a ocorrência de rizóbio. A ocorrência de rizóbio em crotalária e feijão foi estudada em quatro locais, e em guandu em cinco locais. O feijão foi mais específico, seguido por crotalária e guandu.

  10. Structural quality of on Oxisol in recovery for 18 years

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    dos Santos Batista Bonini, C.; Alves, M. C.; Marchini, D. C.; Garcia de Arruda, O.; Nilce Souto Filho, S.

    2012-04-01

    Incorrect use of soil and large buildings construction in rural areas are causing changes to it, making them less productive and thus increasing the degraded areas. Techniques aimed at ecological restoration of degraded soils have been investigated. In this sense we investigated the positive changes in the structural quality of a soil that was beheaded in human intervention techniques for recovery for 18 years, having been used green manures, gypsum and pasture. The studied area is located in Mato Grosso do Sul, Brazil. The experimental design was a completely randomized with seven treatments and four replications. The treatments were: control (tilled soil without culture); Stizolobium aterrium; Cajanus cajan; lime+S. aterrimum; lime+C. cajan; lime+gypsum+S. aterrimum; lime+gypsum+C. cajan. In 1994, all treatments with C. cajan were replaced by Canavalia ensiformis and in 1999, Brachiaria decumbens was implanted in all treatments. Data from vegetated treatments were compared with the control bare soil and native vegetation (savannah). We evaluated the distribution and aggregate stability in water, soil samples were collected in 2010 in the depths: 0.00-0.10; 0.10-0.20 and 0,20-0.40 m. The results were analyzed by analysis of variance, following Scott-Knott test (5%) of probability to compare averages. Evaluating the results is noted that in the depth of 0.00-0.10 m, the control bare soil and savannah soil had lower and higher DMP, respectively. All recovery treatments were DMP greater than found for the bare soil control. Treatments: S. aterrimum, lime + gypsum + C. cajan and lime + gypsum + S. aterrimum and the savannah control were similar in the depth of 0.00-0.10 m. All of the recovery treatment in the depth from 0.00-0.10 m with values is close to the native vegetation of the savannah. Depths of 0.10-0.20 and 0.20-0.40 m results obtained for DMP treatments in recovery are similar to the bare soil, except for treatments with S. aterrimum and lime + gypsum + S

  11. Seleção de espécies de adubos verdes visando à fitorremediação de diclosulam Selection of green manure species aiming at diclosulam phytoremediation

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    P.A Monquero

    2013-03-01

    Full Text Available Espécies de adubos verdes tolerantes ao herbicida diclosulam podem ser utilizadas em rotação de culturas para diminuir o efeito fitotóxico subsequente desse herbicida em plantas sensíveis, como o girassol ou milho. Este estudo teve como objetivo avaliar a tolerância de adubos verdes ao diclosulam e a capacidade dessas plantas em diminuir o efeito fitotóxico do herbicida no bioindicador Helianthus annuus. Foram avaliadas, em casa de vegetação, três doses do herbicida diclosulam (0, 0,035 e 0,070 kg i.a. ha-1 em pré-emergência dos adubos verdes Dolichos lablab, Cajanus cajan, Canavalia ensiformis, Crotalaria juncea, C. breviflora, C. spectabilis, Mucuna deeringiana, M. cinerea, M. aterrima, Lupinus albus, Helianthus annuus, Pennisetum glaucum, Avena strigosa, Raphanus sativus e Calopogonium muconoides. Entre estas espécies, C. cajan, C. ensiformis, M. cinerea e M. aterrima foram selecionadas como as mais tolerantes, sendo avaliadas no campo com o herbicida diclosulam nas doses de 0, 0,035 e 0,070 kg i.a. ha¹, em esquema fatorial 4 x 3 e quatro repetições. A parte aérea dessas plantas foi coletada após 60 dias da emergência, sendo semeado H. annuus como bioindicador do herbicida diclosulam. Os resultados evidenciaram C. cajan como a espécie mais promissora em diminuir o efeito fitotóxico do diclosulam em culturas agrícolas sensíveis.The tolerance of green manure species to diclosulam can be used in crop rotation schemes aiming to reduce the subsequent phytotoxic effect of this herbicide on sensitive plants, such as sunflower or corn plants. This study evaluated the tolerance of green manure to diclosulan and the capacity of these plants in reducing the phytotoxic effect of this herbicide on Helianthus annuus (sunflower. Three rates of diclosulam (0; 0.035, and 0.070 kg a.i. ha-1 were evaluated under greenhouse conditions in pre-emergence of the following green manures: Dolichos lablab, Cajanus cajan, Canavalis ensiformis

  12. Forms of phosphorus in an oxisol under different soil tillage systems and cover plants in rotation with maize

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    Arminda Moreira de Carvalho

    2014-06-01

    Full Text Available Phosphorus fractions play a key role in sustaining the productivity of acid-savanna Oxisols and are influenced by tillage practices. The aim of this study was to quantify different P forms in an Oxisol (Latossolo Vermelho-Amarelo from the central savanna region of Brazil under management systems with cover crops in maize rotation. Three cover crops (Canavalia brasiliensis, Cajanus cajan (L., and Raphanus sativus L. were investigated in maize rotation systems. These cover crops were compared to spontaneous vegetation. The inorganic forms NaHCO3-iP and NaOH-iP represented more than half of the total P in the samples collected at the depth of 5-10 cm during the rainy season when the maize was grown. The concentration of inorganic P of greater availability (NaHCO3-iP and NaOH-iP was higher in the soil under no-tillage at the depth of 5-10 cm during the rainy season. Concentrations of organic P were higher during the dry season, when the cover crops were grown. At the dry season, organic P constituted 70 % of the labile P in the soil planted to C. cajan under no-tillage. The cover crops were able to maintain larger fractions of P available to the maize, resulting in reduced P losses to the unavailable pools, mainly in no-tillage systems.

  13. 干热河谷典型区土壤功能对不同植被恢复措施的响应%Response of Soil Functioning to Ecological Restoration Practices in the Typical Arid-Hot Valley

    Institute of Scientific and Technical Information of China (English)

    纪中华; 李建增; 闫帮国; 钱坤建; 潘志贤; 奎建蕊

    2012-01-01

    To explore the response of soil function in arid-hot ecosystem to restoration treatments,the soil infiltration,physical and chemical properties under different restoration models were measured.The results showed that mixture plantation-Cajanus cajan+Pennisetum purpureum had the most effective function on soil in Tamarindus indica forest.The proportion of soil aggregation increased up to 60%~80%,organic matter of soil surface increased by a factor up to 5.01 times,infiltration rate increased by a factor up to 14.43 times compared to the control(T.indica only).The maximum water holding storage reached up to 633.0 t/hm2.All of the soil characters measured in the C.cajan+P.purpureum model were obviously higher than other models and this also enhanced T.indica productivity.The soil functions of other models were poorer than C.cajan+P.purpureum,but better than the control.The results indicated that the improving effects of soil function were determined by plant species and their combinations.%为研究干热河谷地区不同恢复措施下土壤功能的变化,分析了不同模式下土壤入渗和理化特征。结果表明,在乡土树种罗望子(Tamarindus indica)林下,木豆(Cajanus cajan)+象草(Pennisetum purpureum)模式的恢复效果最好,土壤团粒达60%~80%,表层有机质含量较对照提高了5.01倍,其入渗率较对照区提高14.43倍,最大吸持贮水量可达633.0t/hm2,明显高于其他模式,土壤性质的改善也提高了罗望子的生产力水平。其他恢复模式的土壤功能较差,但优于对照模式,表明土壤理化性质的改良取决于植物种类的选择和配置。

  14. Fitomassa de adubos verdes e cobertura do solo na região do Alto Vale do Jequitinhonha, Minas Gerais = Biomass of green manure and soil cover in the region of Vale do Jequitinhonha, Minas Gerais-Brazil.

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    Evander Alves Ferreira

    2012-08-01

    Full Text Available A adubação verde constitui prática cultural de relevante impacto positivo em sistemas de produção agrícola que visam o uso sustentável da terra. Objetivou-se com esse trabalho avaliar o desempenho de seis leguminosas utilizadas para adubação verde, quanto à produtividade de fitomassa, cobertura do solo e uso da radiação fotossinteticamente ativa na região conhecida como Alto Vale do Jequitinhonha em Minas Gerais. O experimento foi conduzido entre os meses de março a outubro de 2010, em delineamento em blocos casualizados com quatro repetições. Os adubos verdes utilizados foram: crotalária (Crotalariaspectabilis, feijão-de-porco (Canavalia ensiformis, feijão-guandu-anão (Cajanus cajan, lab-lab (Lablab purpureus, mucuna-anã (Mucuna deenringiana e tremoço-branco (Lupinus albus L.. Os adubos verdes apresentaram elevadas produções de fitomassa, mas com diferentes picos de cobertura do solo e interceptação da radiação. Os maiores acúmulos de matéria seca e fresca foram observados pela crotalária seguido pelo feijão-guandu-anão. Entre as espécies estudadas, o feijão-de-porco é aque apresentou maior período vegetativo, representando elevado potencial para recobrimento do solo, destacando-se também na interceptação de radiação fotossinteticamente ativa.Green manuring is a positive practice that reduces the impact on agricultural production systems aimed at sustainable land use. The objective of this study was to evaluate the performance of six legumes used for green manure, the productivity of biomass, land cover and use of photosynthetic active radiation in the region of the Vale do Jequitinhonha, Minas Gerais, Brazil. The experiment was conducted during March to October, 2010 in randomized block design with four replications. Green manures were used: Crotalaria spectabilis, Canavalia ensiformis, Cajanus cajan, Lablab purpureus, Mucuna deenringiana and Lupinus albus. Green manures had high yields of biomass

  15. Efeitos de quantidades de fitomassa de adubos verdes na supressão de plantas daninhas Effects of cover crop biomass quantities on weed suppression

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    F.J. Severino

    2001-08-01

    Full Text Available Com o objetivo de avaliar as correlações entre a utilização de diferentes quantidades de fitomassa, incorporada ou na superfície do solo, dos adubos verdes amendoim-forrageiro (Arachis pintoi, crotalária (Crotalaria juncea e feijão-guandu-anão (Cajanus cajan na redução da emergência e produção de fitomassa seca das plantas daninhas capim-braquiária (Brachiaria decumbens, capim-colonião (Panicum maximum e picão-preto (Bidens pilosa, foi instalado um experimento em casa de vegetação do Departamento de Produção Vegetal da ESALQ/USP-Piracicaba. O delineamento experimental utilizado foi o de blocos casualizados, com os tratamentos em quatro repetições, em esquema fatorial 3 x 6 x 3 x 2, sendo três adubos verdes (A. pintoi, C. juncea e C. cajan, seis quantidades de fitomassa (0; 0,5; 1; 2; 4; e 8 vezes a quantidade de adubo verde produzido pela média de campo, três plantas daninhas (B. decumbens, P. maximum e B. pilosa e duas formas de distribuição da fitomassa dos adubos verdes (na superfície do solo e de 0 a 5 cm de profundidade. As características avaliadas durante a condução do experimento foram as seguintes: densidade de plantas daninhas/vaso e produção de fitomassa seca das plantas daninhas (g/vaso, determinadas aos 15 e aos 30 dias após a instalação do experimento. Os resultados permitiram concluir que a fitomassa dos adubos verdes, nas duas formas utilizadas, reduz significativamente as populações das plantas daninhas testadas. As correlações ocorrem de maneira distinta pelos diferentes adubos verdes, sendo o feijão-guandu-anão, de forma geral, o mais efetivo na supressão das plantas daninhas.Different biomass quantities of the cover crops Arachis pintoi Krapov. & W.C. Gregory, Crotalaria juncea and Cajanus cajan were either incorporated or spread on the surface of the soil to study their effect on the emergence and dry mass production of the weeds Brachiaria decumbens, Panicum maximum and Bidens

  16. 转基因抗虫棉田棉铃虫庇护所的建立和评价%Establishment and Evaluation of Refuge for Helicoverpa Armigera Hübner of Bt Cotton Fields

    Institute of Scientific and Technical Information of China (English)

    吴莉莉; 王登元; 吕昭智; 马睿

    2011-01-01

    [目的]对转基因棉田棉铃虫庇护所的植物进行选择.[方法]通过采取随机区组排列方式在转基因抗虫棉田种植庇护所植物,种植植物为:鹰嘴豆、木豆、玉米、胡麻、高粱、棉花等6种植物,并进行定期、定点、定株调查,比较分析不同植物种类棉铃虫卵和幼虫的数量差异.[结果]鹰嘴豆作为庇护所植物最好,其次是木豆、玉米、胡麻、高粱效果较差.鹰嘴豆的引诱效果与其他5种植物有极显著差异.几种诱集植物能够蓄养大量的草岭、瓢虫、蜘蛛等天敌昆虫.[结论]鹰嘴豆、木豆、玉米作为转基因棉田棉铃虫庇护所植物较好.%[Objective]This study was conducted to select the plants of refuge on Bt cotton fields’ Helicoverpa armigera (H(u)bner) .[Method]Planting refuge in the Bt cotton fields by the way of a random block arrangement, plants are: chickpea’( Cicer arietinum Linn. ) , pigeonpea ( Cajannus cajan L. Mill,) , com ( Zea mays L.), benne( Linseed), grain sorghum( Sorghum vulgare Pers.), cotton( Gossypium spp.) 6 species of plants, whose fixed date,fixed point and fixed plants were surveyed, compare and analyse the quantity difference of Helicoverpa armigera eggs and larvae in different plant were comparatively analyzed . [ Resultr]The results showed tlat chickpea were best as refuge plant; pigeonpea and com followed . benne, grain sorghum leas than other plant.The analysis of variance showed that chickpea had a significant difference with other five plants . These host plants can provide a lot of natural enemies : lacewing, ladybird beetle, spider, etc. [ Conclusion] Chickpea, Pigeonpea, Com can be some better host plant for refuge of Helicoverpa armigera (Hubner) on Bt Cotton Fields.

  17. Recuperação do subsolo em área de empréstimo usada para construção de hidrelétrica Subsoil reclamation in loan area used for hydroelectric construction

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    Marlene Cristina Alves

    2011-06-01

    , degraded by soil removal for hydroelectric power plant construction, using green manure and applying lime and gypsum. A randomized block design was used with seven treatments and four replications. The research was started in 1992 and the treatments were: Control; Cajanus cajan until 1994 followed by Canavalia ensiformis; lime + Stizolobium aterrimum; lime + Cajanus cajan until 1994 and followed by Canavalia ensiformis; lime + gypsum + Stizolobium aterrimum and lime + gypsum + Cajanus cajan until 1994 and followed by Canavalia ensiformis. The treatments consisted of green manure, lime and gypsum. Seven years later, in 1999, the attributes analyzed were: total soil porosity, macroporosity, microporosity, soil bulk density, organic matter and brachiaria dry matter production. The treatments are contributing to the recovery of physical attributes of exposed subsoil, and the treatment with Stizolobium aterrimum without soil correction seems to be the most promising. Soil recuperation effects can be seen on the 0.00-0.10 m layer.

  18. Potencial alelopático de plantas de cobertura no controle de picão-preto (Bidens pilosa L. Cover plants allelopathic potential in Bidens pilosa L. control

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    Cícero Monti Teixeira

    2004-06-01

    Full Text Available Extratos aquosos da parte aérea de mucuna-preta (Stilozobium aterrimum Piper & Tracy, mucuna rajada (Stilozobium sp., Crotalaria juncea L., Crotalaria spectabilis Roth, guandu e guandu anão (Cajanus cajan (L. Druce foram preparados com o objetivo de se determinar a potencialidade alelopática dessas espécies, largamente utilizadas como plantas de cobertura, sobre sementes de alface (planta-teste e aquênios de picão-preto (Bidens pilosa L.. Os experimentos foram conduzidos no Laboratório de Análise de Sementes da Universidade Federal de Lavras, durante o mês de abril de 2003. Foram avaliados o índice de velocidade de germinação (IVG e a germinação final. No experimento com alface, houve redução significativa da germinação apenas para o extrato aquoso de crotalária juncea. Para as outras espécies, houve uma tendência de redução na germinação, porém, não diferindo da testemunha com água destilada. O IVG apresentou redução significativa nos tratamentos com extratos aquosos de crotalária juncea, guandu comum, mucuna-rajada e mucuna-preta. As outras espécies tiveram uma tendência em reduzir o IVG, porém, não diferindo da testemunha. No experimento com picão-preto, a germinação apresentou comportamento semelhante ao experimento com alface, com redução significativa apenas para o tratamento com crotalária juncea, porém, para o IVG, houve redução significativa apenas para a mucuna-preta, com uma tendência de redução para os tratamentos com as outras espécies, mas não diferindo da testemunha.Aqueous extracts from aerial part of black mucuna (Stilozobium aterrimum Piper & Tracy, striped mucuna (Stilozobium sp, Crotalaria juncea L., Crotalaria spectabilis Roth., guandu (Cajanus cajan (L. Druce and dwarf guandu (Cajanus cajan were prepared with the objective of studying the allelopathic potential of these species, broadly used as cover plants, on lettuce seeds (test plant and aquenes of Bidens pilosa L.. The

  19. Produção de fitomassa de diferentes espécies de cobertura e suas alterações na atividade microbiana de solo de cerrado Phytomass yield of different cover crops and alterations in the microbial activity in a cerrado soil in Brazil

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    Marco Aurélio Carbone Carneiro

    2008-01-01

    Full Text Available O objetivo deste estudo foi avaliar a produção de fitomassa de nove espécies de cobertura do solo e verificar as alterações na atividade e biomassa microbiana de solo de cerrado. O trabalho foi desenvolvido de março a dezembro de 2004, em Latossolo Vermelho distroférrico, sendo semeadas as seguintes espécies de cobertura: aveia-preta (Avena strigosa, guandu (Cajanus cajan, Crotalaria juncea, lablabe (Lablab purpureum, milheto (Pennicetum americanum, nabo forrageiro (Raphanus sativus, níger (Guizotia abyssinica (L.F. Cass., feijão-de-porco (Canavalia ensiformis. e Crotalatia spectabilis. Uma área em pousio foi considerada como referência. Na ocasião do florescimento, determinou-se a quantidade de fitomassa e, a partir de agosto, parte dela foi acondicionada em sacos de polietileno, ficando no solo por 120 dias, para se avaliar a razão de decomposição. Durante esse período, no inicio de cada mês, foram avaliados o carbono da biomassa microbiana e a respiração microbiana do solo. As espécies mais promissoras para produção de fitomassa foram o guandu, milheto, feijão-de-porco e niger. O carbono da biomassa microbiana foi negativamente afetado nas áreas com resíduos de milheto, nabo forrageiro, crotalária espectábilis e naquela em pousio. Na área sob pousio, verificou-se a menor produção de fitomassa, além da redução no carbono da biomassa microbiana e mais perda de carbono, sendo esta prática não indicada para a região de cerrado.The objective of this study was to evaluate the phytomass yield of nine species of cover crops of the soil and to verify the alterations in the activity and microbial biomass of Cerrado soil, in the State of Goiás, Brazil. The work was developed from March to December, 2004, being sowed the following covering species: Avena strigosa, Cajanus cajan, Crotalaria juncea, Lablab purpureum, Pennicetum americanum, Raphanus sativus, Guizotia abyssinica, Canavalia ensiformis, Crotalatia

  20. Reciclagem e incorporação de nutrientes ao solo pelo cultivo intercalar de adubos verdes em pomar de laranjeira-'Pêra' Reciclying and incorporation of nutrients to the soil in orchard of 'Pera' orange by cultivation with cover crops

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    JOSÉ ANTONIO ALBERTO DA SILVA

    2002-04-01

    Full Text Available O experimento de campo foi conduzido na Estação Experimental de Citricultura de Bebedouro-SP (EECB, visando a avaliar a produção de matérias verde e seca e quantidade de nutrientes incorporados ao solo pelo cultivo intercalar de Crotalaria juncea, Crotalaria spectabilis, Cajanus cajan, Mucuna aterrima, Mucuna deeringiana, Dolichos labe-labe e Canavalia ensiformis, nos anos agrícolas de 1989/90, 90/91, 91/92 e 92/93, em pomar de laranjeira-'Pêra' (Citrus sinensis L. Osbeck, enxertada sobre tangerineira-'Cleópatra' (Citrus reshi Hort., plantada em maio de 1987 e espaçadas de 7x5 m, num Latossolo Vermelho-Escuro. A produtividade média das espécies plantadas sem adubações e cultivos pós-semeadura, nos quatro anos de estudo, foram: 6,55; 1,23; 3,42; 1,78; 1,75; 1,61 e 3,03 t/ha de matéria seca, respectivamente, considerando plantio apenas na área intercalar de citros (50% da área total. A análise química do material seco revelou a incorporação de volume considerável de N, P2O5, K2O, Ca, Mg, S, B, Cu, Fe, Mn e Zn pelas leguminosas, onde podemos considerar que parte do N é proveniente da fixação simbiótica do N2 atmosférico pelas leguminosas e o restante e demais nutrientes provenientes do importante processo de reciclagem de nutrientes do solo, que são absorvidos pelas leguminosas das camadas subsuperficiais e incorporados na superfície do solo, onde estarão novamente disponíveis às plantas cítricas. A C. juncea foi a espécie que se destacou como maior produtora de biomassa e incorporadora de nutrientes, seguida pelo C. cajan e C. ensiformis.The experiment was carried out at the Experimental Station of Bebedouro-SP (EECB, to evaluate the production of green manure and incorporated nutrients to soil by inter-cropping Crotalaria juncea, Crotalaria spectabilis, Cajanus cajan, Mucuna aterrima, Mucuna deeringiana, Dolichos labe-labe and Canavalia ensiformis, in the agricultural years of 89/90, 90/91, 91/92 and 92/93, in

  1. Chemical composition, “in vitro” digestibility and production of woody forage legumes cultivated in sandy soils Composição bromatológica, digestibilidade "in vitro" e produção de biomassa de leguminosas forrageiras lenhosas cultivadas em solo arenoso

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    Edimilson Volpe

    2009-09-01

    Full Text Available Considering the nutritional value and productivity of woody forage legumes Albizia lebbeck, Cajanus cajan, Cratylia argentea, Gliricidia sepium e Leucaena leucocephala, to be used in supplementation of cattle as a bank protein, five species in Quartzpisament were evaluated. The experiment was conducted from March 2006 to October 2007. The evaluations were carried out in two periods (rainy and dry, with two cuts by period. There was no difference in the accumulation of dry matter, in rainy period. In the dry evaluation, A. lebbeck and C. argentea were higher than the other legumes. The C. cajan was not recovered after the rainy period, which resulted in a low accumulation of dry matter in the dry period. The leaves of Albizia lebbeck showed the highest levels of crude protein in cuts evaluated (210 to 212 g/kg. The in vitro digestibility of dry matter in the leaves of A. lebbeck was significantly higher, with average values of 568g/kg. A. lebbeck, Cratylia argentea and Gliricidea sepium showed the highest content for digestibility of the thin stem. The A. lebbek and C. argentea presented higher accumulation of dry matter (19,3 and 24,3 t DM/ha, respectively and, still, the best values for the qualitative characteristics. Both species are the most promising and adapted to the formation of protein banks in these types of soils.Com o objetivo de avaliar o valor nutritivo e a produtividade das leguminosas forrageiras lenhosas: Albizia lebbeck (Albízia, Cajanus cajan (Feijão-Guandu, Cratylia argentea (Cratília, Gliricidia sepium (Gliricídia e Leucaena leucocephala (Leucena, a serem utilizadas na suplementação de bovinos como banco de proteína, conduziu-se um experimento em Neossolo Quartzarênico. O experimento foi realizado entre março de 2006 e outubro de 2007. As avaliações foram realizadas em dois períodos (chuvoso e seco, com dois cortes para cada período. Não houve diferença no acúmulo de matéria seca nos cortes do per

  2. Produção de biomassa e teor de macronutrientes do milheto, feijão-de-porco e guandu-anão em cultivo solteiro e consorciado Biomass production and macronutrients quantity of millet, jack bean, guandu single and in intercropping

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    Cícero Monti Teixeira

    2005-02-01

    Full Text Available Objetivou-se determinar a produção de biomassa e o teor de macronutrientes do milheto (Pennisetum typhoides (Burm. Stapf, feijão-de-porco (Canavalia ensiformes (L. DC. e guandu-anão (Cajanus cajan (L. Millsp. em cultivo solteiro e nos consórcios da gramínea com as leguminosas, visando a produção de palha no sistema plantio direto. O trabalho foi conduzido no campo experimental do Departamento de Agricultura da Universidade Federal de Lavras (Lavras, MG, Brasil. O delineamento foi o de blocos casualizados, com cinco tratamentos e quatro repetições. Apenas o guandu-anão solteiro apresentou menor produção de fitomassa fresca e seca, sendo que os demais tratamentos não diferiram entre si, pelo teste de Tukey a 5% de probabilidade. De forma geral, as leguminosas apresentaram maiores teores de N e Ca e o milheto maiores teores de S. Apenas o feijão-de-porco solteiro apresentou menor teor de P. Os maiores teores de K foram apresentados pelo milheto solteiro e consorciado com as leguminosas e pelo feijão-de-porco em consórcio com milheto. Os menores teores de Mg foram verificados para o guandu-anão.The object of this work was to evaluate the biomass production and the macronutrients quantity of millet (Pennisetum typhoides (Burm. Stapf, jack bean (Canavalia ensiformes (L. DC., guandu (Cajanus cajan, (L. Millsp. dwarf cultivar, and the millet with leguminous consortiums to straw production in no-tillage system. The experiment was carried in experimental area at Agriculture Department of the Federal University of Lavras (Lavras, Minas Gerais state, Brazil. The experimental design was randomized blocks, with five treatments and four replications. For biomass production, only the guandu presented smaller fresh and dry biomass yield than the other treatments. In general, the leguminous presented the greatest N and Ca quantities and millet the greatest quantities of S. Only jack bean presented minor P quantity. For K the greatest quantities

  3. Composição química e contribuição nutritiva de cecotrofos de diferentes dietas Chemical composition and nutritive contribution of cecothrophes from different diets

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    Augusto Vidal da Costa Gomes

    1999-01-01

    Full Text Available Os efeitos de cinco fontes de fibra sobre a produção, composição química e a contribuição nutritiva dos cecotrofos em termos de matéria seca e proteína bruta foram estudados. Quarenta coelhas da raça Nova Zelândia branco foram alojadas em gaiolas metabólicas, por 12 dias, para adaptação às dietas. No 13º dia, para evitar a coprofagia, os animais receberam um colar de madeira por um período de 24 horas, procedendo-se à coleta dos cecotrofos de duas em duas horas. A dieta padrão tinha como principal fonte de fibra o feno de alfafa. As outras dietas foram caracterizadas pela substituição isométrica do feno de alfafa por feno de guandu (Cajanus cajan, palha de feijão, palha e sabugo de milho branco e o feno coast-cross (Cynodon dactylon. Foi observado efeito do tipo de dieta sobre a composição química dos cecotrofos e o teor de PB ingerida por cecotrofia, porém a produção de cecotrofos não diferiu entre dietas.The effects of five fiber sources on the production, chemical composition and cecotrophes nutritive contribution on dry matter and crude protein base were studied. Forty White New Zealand female rabbits were housed in metabolic cage for 12 days to adaptation to the diets. In the 13th day, to prevent coprophagy, the animals received a necklace of wood for a period of 24 hours, and the cecotrophes were collected every two hours. The standard diet had the alfalfa hay as a mainly fiber source. The other diets were characterized by isometric replacement of alfalfa hay by pigeon pea hay (Cajanus cajan, bean straw, white corn with cob and husks and coast-cross hay (Cynodon dactylon. Effect of diet type on cecotrophes chemical composition and crude protein content ingested by cecotrophy was observed, however the cecotrophes production was not different among diets.

  4. PRODUTIVIDADE DO CAFÉ APOATÃ EM CONSÓRCIO COM LEGUMINOSAS NA REGIÃO DA ALTA PAULISTA COFFEE PRODUCTIVITY DURING INTERCROPPING WITH FIVE LEGUMINOUS SPECIES IN THE WESTERN REGION OF SÃO PAULO STATE, BRAZIL

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    EDISON MARTINS PAULO

    2001-01-01

    Full Text Available Avaliou-se, no Núcleo de Agronomia da Alta Paulista, no período de 1991 a 1993, a produção de café Apoatã IAC 2258 (Coffea canephora Pierre submetido ao plantio intercalar dos adubos verdes: crotalária júncea (Crotalaria juncea L., crotalária espectabilis (Crotalaria spectabilis Roth., mucuna-anã (Stizolobium deeringeanum Bort., soja IAC 9 [Glycine max (L. Merrill] e guandu [Cajanus cajan (L. Millsp.], incorporados no seu florescimento. As leguminosas foram plantadas a 50 cm da projeção da copa das plantas de café. Adotou-se o delineamento estatístico de blocos ao acaso com cinco repetições. Os resultados mostraram que o guandu e a crotalária júncea reduziram a produção de café, e o guandu, a altura e o diâmetro do caule do cafeeiro, o que não ocorreu com as demais leguminosas. As maiores quantidades de fitomassa seca foram produzidas por guandu e crotalária júncea respectivamente. A produção de café correlacionou-se inversamente com a fitomassa seca das leguminosas e, positivamente, com a altura e o diâmetro do caule do cafeeiro.Coffee (Coffea canephora Pierre productivity was evaluated during three years of intercropping with five leguminous species: sunn hemp (Crotalaria juncea L., Crotalaria spectabilis Roth., dwarf velvet bean (Stizolobium deeringeanum Bort., soybean [Glycine max (L. Merryl] and pigeon pea [Cajanus cajan (L. Millsp.] in the western region of São Paulo State, from 1991 to 1993. Leguminous species were seeded 50 cm far from coffee shoot and incorporated at flowering stage. The treatments were arranged in a randomized complete block design with five replicates. Coffee yield was not affected when Crotalaria spectabilis, dwarf velvet bean and soya bean IAC 9 were used as interrow crop, but it was significantly decreased for pigeon pea and sunn hemp treatments. Linear correlation analysis showed that coffee yield was inversely correlated with leguminous dry biomass and positively correlated with

  5. BANCO DE SEMENTES DE PLANTAS DANINHAS EM SOLO CULTIVADO COM ADUBOS VERDES WEED SEED BANK IN GREEN-MANURE-CULTIVATED FIELDS

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    FRANCISCO JOSÉ SEVERINO

    2001-01-01

    Full Text Available Com o objetivo de estudar a composição de banco de sementes de plantas daninhas em solo cultivado com adubos verdes, desenvolveu-se um experimento em casa de vegetação do Departamento de Produção Vegetal da Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP, Piracicaba (SP, entre dezembro de 1999 e fevereiro de 2000. As parcelas experimentais, compostas de 1 kg de solo amostrado por um trado de 4,3 cm de diâmetro, a partir da experimentação em campo, foram instaladas em bandejas de 30 x 20 x 5 cm. O delineamento experimental adotado foi o de blocos ao acaso com quatro repetições constando dos fatores: a adubos verdes - amendoim-forrageiro (Arachis pintoi Krapov. & W.C. Gregory, crotalária (Crotalaria juncea L., guandu-anão [Cajanus cajan (L. Millsp.] e testemunha sem capina; b plantas daninhas - capim-braquiária (Brachiaria decumbens Stapf., capim-colonião (Panicum maximum Jacq., picão-preto (Bidens pilosa L., além da vegetação espontânea. Avaliou-se o número de plantas daninhas emergidas aos 30 e 60 dias após a instalação do experimento. Os resultados permitem concluir que o banco de sementes de plantas daninhas foi significativamente reduzido com a utilização dos adubos verdes, constituindo em prática agrícola relevante para o manejo integrado de plantas daninhas.In order to evaluate the composition of a weed seed bank in a soil cultivated with green manure, an experiment was carried out from December, 1999, to February, 2000, in a greenhouse of the Plant Production Department, ESALQ/University of São Paulo, Piracicaba, Brazil. The experimental units were made of 30 x 20 x 5 cm trays, filled with 1.0 kg of soil. The soil was collected by a 4.3 cm core sampler from the experimental field where the green manure and weeds were grown. The experiment had a completely randomized block design with four replicates, green manure (Arachis pintoi Krapov. & W.C. Gregory, Crotalaria juncea L. and Cajanus cajan L

  6. Effect of plant and fungous metabolites on Meloidogyne exigua Efeito de metabólitos vegetais e fúngicos sobre Meloidogyne exigua

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    Daniel Rufino Amaral

    2009-01-01

    Full Text Available As nematodes cause great damage to Brazilian coffee production, effective methods to control these parasites are necessary. In a previous work Allium cepa L., Cajanus cajan (L. Mill., Crotalaria juncea L., Ficus elastica Roxb., Ruta graveolens L., Stylosanthes guianensis Aubl., Leucaena leucocephala (Lam. Dewit., Brachiaria decumbens Stapf., Catharanthus roseus G. Don, Tagetes minuta L., Ricinus communis L. and Coffea arabica L. produced active substances against Meloidogyne exigua Goeldi, a nematode widely disseminated through Brazilian coffee fields. Thus, aqueous extracts of such plants, collected in a different season from that of the previous work, as well as crude metabolites produced in liquid medium by Fusarium moniliforme Shelden and Cylindrocarpon magnusianum (Sacc. Woll., were submitted to in vitro assays with M. exigua second-stage juveniles (J2. All plants and fungi produced active substances against J2. Therefore, their metabolites were applied to six-month-old coffee plants inoculated with M. exigua. After 90 days in a greenhouse, those samples obtained from A. cepa, L. leucocephala, R. graveolens and F. moniliforme inhibited the production of galls and eggs by M. exigua, demonstrating potential to control such parasite.Os nematóides acarretam grandes perdas aos produtores brasileiros de café, sendo necessário o desenvolvimento de métodos eficientes para o seu controle. Em trabalho anterior, Allium cepa L., Cajanus cajan (L. Mill., Crotalaria juncea L., Ficus elastica Roxb., Ruta graveolens L., Stylosanthes guianensis Aubl., Leucaena leucocephala (Lam. Dewit., Brachiaria decumbens Stapf., Catharanthus roseus G. Don, Tagetes minuta L., Ricinus communis L. e Coffea arabica L. produziram substâncias ativas contra o nematóide Meloidogyne exigua Goeldi, que é amplamente disseminado pelos cafezais brasileiros. Dando continuidade a esse trabalho, extratos aquosos das plantas mencionadas, coletadas em época diferente daquela

  7. Especificidade hospedeira de variantes Bradyrhizobium spp em soja (cvs peking e clark, caupi e guandu Host specificity of Bradyrhizobium spp mutants in soybean (cultivars peking and clark, cowpea and pigeon pea

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    Fabíola Gomes de Carvalho

    2008-12-01

    Full Text Available A expansão da cultura da soja evidenciou uma alta especificidade hospedeira, requerendo a pesquisa de novas estirpes que apresentassem capacidade de nodular a soja e bom potencial de competição com a população de rizóbios naturalizada nos solos. O objetivo deste trabalho foi avaliar a especificidade hospedeira de variantes isolados de estirpes de Bradyrhizobium spp quanto à nodulação e eficiência relativa de fixação de N2 atmosférico em soja (cvs peking e clark, caupi e guandu. O experimento foi realizado sob condições controladas em câmara de crescimento por meio de testes de variantes de B. japonicum e B. elkanii e suas respectivas estirpes originais quanto à habilidade de nodular soja, caupi e guandu. A colheita foi realizada aos 35 dias, sendo avaliada a nodulação (número, peso dos nódulos secos, produção de matéria seca na parte aérea, eficiência relativa de fixação de N2 atmosférico. Os variantes e estirpes de Bradyrhizobium spp nodularam Glycine max (cultivares BR-16, Clark e Peking, Vigna unguiculata e Cajanus cajan, contudo, apenas para Glycine max a interação rizóbio-leguminosa demonstrou eficiência simbiótica significativa.The expansion of the soybean crop has evidenced a high host specificity, indicating the requirement of new strains with capacity of nodulating soybean and competition capacity with the natural soil rhizobial population. The purpose of this study was to evaluate the host specificity of mutants isolated from Bradyrhizobium spp strains in relation to nodulation and relative N2 fixation effectiveness in soybean (cultivars Peking and Clark, cowpea and pigeon pea. The experiment was carried out under controlled conditions in a growth chamber, where B. japonicum and B. elkanii mutants and the respective original strains were tested for their nodulation ability on soybean, cowpea and pigeon pea. The crop was harvested 35 days after planting and the nodulation (number, dry nodule weight

  8. Degradabilidade ruminal do feno de alguns alimentos volumosos para ruminantes Ruminal degradability of some roughage hays for ruminants feeding

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    G.G.P. Carvalho

    2006-08-01

    Full Text Available Avaliou-se a degradabilidade ruminal da matéria seca (MS, proteína bruta (PB, fibra em detergente neutro (FDN, da fibra em detergente ácido (FDA e hemicelulose dos fenos de capim-elefante (Pennisetum purpureum, palma (Opuntia ficus, guandu (Cajanus cajan e parte aérea da mandioca (Manihot esculenta utilizando três bovinos mestiços machos, castrados, canulados no rúmen e mantidos em regime de pasto. Amostras de 4g de cada alimento foram incubadas em duplicata no rúmen dos animais, nos períodos de 0, 6, 12, 24, 36, 48 e 72 horas. A degradabilidade potencial da PB dos fenos de capim-elefante e guandu foi semelhante, 83,9 e 81,2%, respectivamente. Os maiores valores foram observados para os fenos de palma (94,2% e parte aérea da mandioca (91,7%. A degradabilidade efetiva (DE foi obtida considerando as taxas de passagem de 2, 5 e 8%/hora. A maior DE observada para MS (60,5%, PB (81,1%, FDN (21,6%, FDA (27,9% e HEM (58,0%, na taxa de passagem de 5%/h, ocorreu com o feno de palma.The ruminal degradability of dry matter (DM, crude protein (CP, neutral detergent fiber (NDF, acid detergent fiber (ADF and hemicellulose (HEM of elephantgrass (Pennisetum purpureum, forage cactus (Opuntia ficus, pigeon pea (Cajanus cajan and cassava foliage (Manihot esculenta hays was evaluated using three cannulated crossbred steers, kept on pasture. Samples of four grams of each hay were incubated in the rumen for 0, 6, 12, 24, 36, 48 and 72 hours. The CP potential degradability (PD for elephantgrass and pigeon pea hays was similar, 83.9 and 81.2%, respectively. Higher values were observed either for forage cactus (94.2% or cassava foliage (91.7% hays. The effective degradability (ED was obtained considering the passage rates of 2, 5 and 8%/hour. The forage cactus hay, at a passage rate of 5%/h, showed the highest ED for DM (60.5%, CP (81.1%, NDF (21.6%, ADF (27.9% and HEM (58.0%.

  9. Degradabilidade ruminal da matéria seca, da fração fibrosa e da proteína bruta de forrageiras Forages dry matter, fibrous fraction and crude protein ruminal degradability

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    Aureliano José Vieira Pires

    2006-04-01

    Full Text Available O objetivo deste trabalho foi avaliar a degradabilidade ruminal da matéria seca, da fibra em detergente neutro, da fibra em detergente ácido e da proteína bruta da alfafa (Medicago sativa, aveia-preta (Avena strigosa, leucena (Leucaena leucocephala e guandu (Cajanus cajan. Amostras de 3 g das forragens foram incubadas no rúmen de três novilhos por períodos de 0, 6, 12, 24, 36, 48 e 72 horas. As degradabilidades efetivas da matéria seca da alfafa e da aveia, para a taxa de passagem de 5% por hora, foram elevadas (acima de 60%. A leucena e o guandu apresentaram valores inferiores, 50,9 e 56,0%, respectivamente. A partir de 24 horas de incubação, a aveia se destacou com maior desaparecimento da fibra em detergente neutro e da fibra em detergente ácido, e ainda apresentou as mais elevadas taxas de degradação efetiva destas frações. A aveia foi a forragem que apresentou maior degradabilidade da matéria seca, da fibra em detergente neutro, da fibra em detergente ácido e da proteína bruta no rúmen. O guandu, entretanto, foi a forragem com as piores taxas de degradação.The objective of this work was to evaluate ruminal degradability of dry matter, neutral detergent fiber, acid detergent fiber and crude protein of alfalfa (Medicago sativa, black oat (Avena strigosa, leucaena (Leucaena leucocephala and pigeon pea (Cajanus cajan. Samples of 3 g of forages were incubated in the rumen of three steers for 0, 6, 12, 24, 36, 48 and 72 hours periods. The dry matter effective degradabilities of alfalfa and oat, for a passage rate of 5%/hour, were high (over 60%. However, leucaena and pigeon pea showed lower values, 50.9 and 56.0%, respectively. From 24-hour incubation period on, the oat presented the highest neutral detergent fiber and acid detergent fiber disappearance and showed the greatest effective degradation rates of these fractions. The oat was the forage with the highest dry matter, neutral detergent fiber, acid detergent fiber and

  10. Fitorremediação do herbicida trifloxysulfuron sodium Phytoremediation of the herbicide trifloxysulfuron sodium

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    J.B. Santos

    2004-06-01

    Full Text Available Este trabalho teve como objetivo avaliar a eficiência de espécies vegetais na fitorremediação do herbicida trifloxysulfuron sodium em solos, utilizando o milho como planta indicadora. Os tratamentos foram compostos pela combinação de espécies (Calopogonium muconoides, Crotalaria juncea, Crotalaria spectabilis, Vicia sativa, Cajanus cajan, Canavalia ensiformis, Helianthus annus, Dolichus lablab, Pennisetum glaucum, Stylosantes guianensis, Mucuna deeringiana, Mucuna cinereum, Mucuna aterrima, Raphanus sativus e Lupinus albus, semeadas anteriormente à cultura do milho, mais um tratamento controle (sem cultivo prévio e três doses do herbicida trifloxysulfuron sodium (0,00; 3,75; e 15,00 g ha-1. O delineamento experimental usado foi o de blocos ao acaso, em esquema fatorial, sendo utilizadas três repetições. A semeadura das espécies vegetais nos vasos foi feita no dia seguinte à aplicação do trifloxysulfuron sodium. Após 80 dias da semeadura, as espécies vegetais foram cortadas na altura do coleto, descartando-se a sua parte aérea. A seguir, foi realizada a semeadura do milho (cultivar AG-122. Aos 45 dias após a emergência das plantas de milho foram avaliadas a altura de plantas e a sua biomassa seca da parte aérea. Verificou-se que as espécies M. aterrima e C. ensiformis foram eficientes na descontaminação do herbicida trifloxysulfuron sodium em solo.This work aimed to evaluate the efficiency of plant species in the phytoremediation of the herbicide trifloxysulfuron sodium in soils, by using corn crop as a bioindicator. The treatments were composed by the combination of the species (Calopogonium muconoides, Crotalaria juncea, Crotalaria spectabilis, Vicia sativa, Cajanus cajan, Canavalia ensiformis, Helianthus annus, Dolichus lablab, Pennisetum glaucum, Stylosantes guianensis, Mucuna deeringiana, Mucuna cinereum, Mucuna aterrima, Raphanus sativus and Lupinus albus sowed before the corn, without previous cultivation (control

  11. Potencial de espécies utilizadas como adubo verde no manejo integrado de plantas daninhas Potential of species used as green manure in the integrated weed management

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    E.A.L. Erasmo

    2004-09-01

    Full Text Available O presente trabalho foi conduzido na Estação Experimental da Universidade Federal do Tocantins, Gurupi-TO, Brasil. O experimento foi instalado com o objetivo de avaliar durante 60 dias, em campo, a interferência de oito espécies utilizadas freqüentemente como adubos verdes (Mucuna aterrima, Mucuna pruriens, Crotalaria ochroleuca, Crotalaria spectabilis, Canavalia ensiformis, Cajanus cajan, Pennisetum americanum e Sorghum bicolor, híbrido BR304 sobre a comunidade infestante. As espécies de plantas daninhas mais freqüentes na área do experimento foram: Digitaria horizontalis, Hyptis lophanta e Amaranthus spinosus. Foram realizadas amostragens aos 15, 30, 45 e 60 dias após a formação da cobertura, utilizando um quadrado de amostragem equivalente a 0,25 m². As plantas daninhas foram devidamente identificadas, coletadas, secas e pesadas. O delineamento experimental utilizado foi inteiramente casualizado e constou de nove tratamentos, com quatro repetições cada. Verificou-se que as espécies C. spectabilis, S. bicolor, C. ochroleuca, M. aterrima e M. pruriens reduziram significativamente o número e o peso da matéria seca da população das plantas daninhas avaliadas (D. horizontalis, H. lophanta e A. Spinosus, principalmente as duas últimas, enquanto P. americanum mostrou-se a menos eficiente nesse aspecto.This work was carried out at the Experimental Station of the University of Tocantins, Gurupi-TO, Brazil, to evaluate the interference of eight species frequently used as green manure (Mucuna aterrima, Mucuna pruriens, Crotalaria ochroleuca, Crotalaria spectabilis, Canavalia ensiformis, Cajanus cajan, Pennisetum americanum and Sorghum bicolor, hybrid BR304 in the weed community, for sixty days under field conditions. The most frequent weed species in the experimental area were Digitaria horizontalis, Hyptis lophanta and Amaranthus spinosus. Samplings were made at 15, 30, 45 and 60 days after formation of green manure covering, using

  12. Potencial de espécies vegetais para a remediação do herbicida trifloxysulfuron-sodium Potential of plant species for remediation of trifloxysulfuron-sodium

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    S.O. Procópio

    2005-03-01

    Full Text Available Este trabalho teve como objetivo avaliar a eficiência de espécies vegetais na remediação do herbicida trifloxysulfuron-sodium em solos, utilizando o feijão (Phaseolus vulgaris como planta indicadora. Os tratamentos foram compostos pela combinação entre as espécies Calopogonium mucunoides, Crotalaria juncea, Crotalaria spectabilis, Vicia sativa, Cajanus cajan, Canavalia ensiformis, Medicago sativa, Dolichus lab lab, Penisetum glaucum, Stylosantes guianensis, Mucuna deeringiana, Mucuna cinereum, Mucuna aterrima, Raphanus sativus e Lupinus albus. Todas as espécies foram semeadas em vasos no dia seguinte à aplicação do trifloxysulfuron-sodium em três doses (0,00; 3,75; e 15,00 g ha-1. Após 80 dias da semeadura, as espécies vegetais foram cortadas na altura do coleto e a parte aérea destas descartada. A seguir, foi realizada a semeadura do feijão (cultivar Pérola. Aos 45 dias após a emergência das plantas de feijão avaliaram-se a altura e a massa seca da parte aérea das plantas. Melhor eficiência na descontaminação do trifloxysulfuron-sodium em solo foi obtida pelas espécies M. aterrima e C. ensiformis.This work aimed to evaluate the efficiency of vegetable species in the remediation of the herbicide trifloxysulfuron-sodium in soils using the common bean (Phaseolus vulgaris as a bio indicator. The treatments were composed by the combination of the species Calopogonium mucunoides,Crotalaria juncea, Crotalaria spectabilis, Vicia sativa, Cajanus cajan, Canavalia ensiformis, Medicago sativa, Dolichus lab lab, Penisetum glaucum, Stylosantes guianensis, Mucuna deeringiana, Mucuna cinereum, Mucuna aterrima, Raphanus sativus and Lupinus albus, sown in vases the day following application of the herbicide at three doses (0.00; 3.75; and 15.00 g ha-1. Eighty days after sowing, the plants were cut and the shoot discarded, followed by bean ("Perola" cultivar sowing. At 45 days post emergence, bean height and shoot dry matter were

  13. Fijación biológica de nitrógeno por tres fabáceas (Leguminosae en suelos ácidos de Tabasco, México

    Directory of Open Access Journals (Sweden)

    S. Córdova-Sánchez

    2011-01-01

    Cajanus cajan L., Canavalia ensiformis L. y Mucuna deerengiana L., en rotación y asociación con maíz durante dos ciclos de cultivo, se desarrollaron dos experimentos en un suelo ácido de la sabana de Huimanguillo, Tabasco. En ambos sistemas se evaluaron variables relacionadas con la nodulación de estas fabáceas, así como su efecto sobre el rendimiento de maíz (Zea mays L.; también se cuantificó el N derivado de la atmósfera (Fbn y la eficiencia de absorción de N por el cultivo de maíz a través de la técnica isotópica de 15N. Los resultados muestran que C. cajan L., produjo 66 a 121, C. ensiformis L., 38 a 80 y M. deerengiana L., 45 a 78 nódulos planta-1, con una ms máxima de 14, 7 y 4 g planta-1 en asociación como en rotación, respectivamente. La producción de biomasa por las fabáceas fue de 5,780 para C. cajan L., 7,037 para C. ensiformis L., y 5,284 kg ha-1 para M. deerengiana L., en asociación con maíz. El rendimiento de grano de los diferentes genotipos de maíz en rotación y asociación varió entre 580 a 642 kg ha-1 durante dos ciclos de estudio. El N fijado por estas fabáceas en rotación se considera muy bajo (2 kg N ha-1; mientras que ocurrió mayor fijación en la asociación fabaceae-maíz (135 kg N ha-1, pero la alta dosis de Fbn no repercutió en mayor rendimiento de grano respecto a los testigos.

  14. Evaluation of N2—Fixing Capacites of Herbaceous and Shrub Legumes Under Field Condition

    Institute of Scientific and Technical Information of China (English)

    XJINGGUANGXI; SHISHULIAN; 等

    1998-01-01

    Natural 15N abundance method was used in this study to investigate the N2-fiing capacities of several herbaceous and shrub legumes by a field experiment,For herbaceous legumes,the results were in consistent with those obtained by a pot experiment .Crotalaria mucronata Desv.had a higher,N2-fixing capacity than Vigna sinensis(L.)Savi.For shurub legumes,N2-fixing Capacity under field condition was slightly different from that in pot experiment.These results demonstrated that the natural 15N abundance method was applicable to evaluating N2-fixing capacities of herbaceous and shrub legumes,and that pot experiment was suitable for evaluating the N2-fixing capacities of not only herbaceous legumes but also shrub legumes. Leguminous N2-fixing plants differed in response to phosphorus fertilization,Phosphorus fertilizer appication greatly increased the percentage of nitrogen derived from air (% N dfa)and total amount of N2 fixed by Lespedez formosa(Vog.)Koehne (Jiangxi),Phosphorus fertilizer showed no signifcant effect on the N2-fixing percentage of Cajanus cajan(L.)Millsp.but increased its total biomass,thus increasing the total amount of N2 fixed.

  15. Feeding and oviposition preference of Phyllophaga cuyabana (Moser) (Coleoptera: Melolonthidae) on several crops.

    Science.gov (United States)

    Oliveira, Lenita J; Garcia, Maria A; Hoffmann-Campo, Clara B; do Amaral, Maria L B

    2007-01-01

    Laboratory and greenhouse experiments were carried out to study food and oviposition preference by Phyllophaga cuyabana (Moser) on different plant species as Cajanus cajan L. (pigeon pea), Crotalaria juncea L. (sun hemp), Crotalaria spectabilis Roth (showy crotalaria), Crotalaria ochroleuca G. Don (slenderleaf rattlebox), Glycine max [L.] Merrill (soybean), Gossypium hirsutum L. (cotton), Helianthus annuus L. (sunflower), Stizolobium aterrimum [Mucuna aterrima] Piper Tracey (velvetbean) and Zea mays L. (mayze). In no-choice experiments, the number of eggs layed in sunflower, C. juncea and soybean was larger compared to cotton. Despite the fact that the adults did not discriminate among plants, in dual-choice test, the proportion of eggs layed and leaf consumption by P. cuyabana adults in soybean were significantly higher than in C. spectabilis. The larval distribution in the soil was at random in multiple-choice, without any trend of preference, but in dual-choice, when soybean was the control, larvae always preferred to feed on its roots. P. cuyabana adults had preference for more suitable hosts and that could stand their offspring survival. This behaviour can be usefully exploited in an integrated management program for this pest.

  16. Cover plants and mineral nitrogen: effects on organic matter fractions in an oxisol under no-tillage in the cerrado

    Directory of Open Access Journals (Sweden)

    Isis Lima dos Santos

    2014-12-01

    Full Text Available Cover plants are essential for the sustainability of no-tillage systems in tropical regions. However, information on the effects of these plants and N fertilization on soil organic matter fractions is still scarce. This study evaluated the effect of cover crops with different chemical composition and of N topdressing on the labile and humified organic matter fractions of an Oxisol of the Cerrado (savanna-like vegetation. The study in a randomized complete block design was arranged in split-plots with three replications. Four cover species were tested in the plots and the presence or absence of N topdressing in the subplot. The following cover species were planted in succession to corn for eight years: Urochloa ruziziensis; Canavalia brasiliensis M. ex Benth; Cajanus cajan (L. Millsp; and Sorghum bicolor (L. Moench. In general, the cultivation of U. ruziziensis increased soil C levels, particularly of C in the humic acid and particulate organic C fractions, which are quality indicators of soil organic matter. The C in humic substances and mineral organic C accounted for the highest proportions of total organic C, demonstrating the strong interaction between organic matter, Fe and Al oxides and kaolinite, which are predominant in these weathered soils of the Cerrado.

  17. Characterization of the bioactive metabolites from a plant growth-promoting rhizobacteria and their exploitation as antimicrobial and plant growth-promoting agents.

    Science.gov (United States)

    George, Emrin; Kumar, S Nishanth; Jacob, Jubi; Bommasani, Bhaskara; Lankalapalli, Ravi S; Morang, P; Kumar, B S Dileep

    2015-05-01

    A plant growth-promoting bacterial strain, PM 105, isolated from a tea plantation soil from the North Eastern region of India was identified as Pseudomonas aeruginosa through classical and 16S ribosomal DNA (rDNA) gene sequencing. Further studies with this strain confirmed broad spectrum antifungal activity against ten human and plant pathogenic fungal pathogens viz. Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus tubingensis, Candida albicans, Colletotrichum gloeosporioides, Fusarium oxysporum, Pencillium expansum, Rhizoctonia solani, Trichophyton rubrum besides growth-promoting property in cowpea (Vigna unguiculata) and pigeon pea (Cajanus cajan). However, no antibacterial property was exhibited by this strain against the four test bacterial pathogens tested in agar overlay method. The crude bioactive metabolites produced by this strain were isolated with three different solvents that exhibited significant antimicrobial and plant growth-promoting activity. Chloroform extract recorded significant antimicrobial and plant growth-promoting activity. Three major compounds viz. 1-hydroxyphenazine, pyocyanin, and phenazine-1-carboxamide were purified and characterized from crude extracts of this strain by various spectral data. The purified compounds recorded prominent antimicrobial activity but failed to establish the plant growth promotion activity in test crop plants under gnotobiotic conditions. Pyocyanin recorded significant antimicrobial activity, and best activity was recorded against T. rubrum (29 mm), followed by P. expansum (28 mm). These results suggest the use of PM 105 as plant growth-promoting agent in crop plants after successful field trials.

  18. Recuperação de área degradada por construção de hidroelétrica com adubação verde e corretivo Soil reclamation by green manure and soil ammendements in areas degraded by hydroelectric power plant construction

    Directory of Open Access Journals (Sweden)

    Marlene Cristina Alves

    2008-12-01

    system in Ilha Solteira, São Paulo, Brazil, by green manure, lime and gypsum. The experiment had a randomized block design with seven treatments and four replications. The treatments consisted of: one control (tilled soil and spontaneous vegetation; Cajanus cajan until 1994 and then substituted by Canavalia ensiformis; lime + Stizolobium aterrimum; lime + Cajanus cajan until 1994 and then substituted by Canavalia ensiformis; lime + gypsum + Stizolobium aterrimum and lime + gypsum + Cajanus cajan until 1994 and then substituted by Canavalia ensiformis. After four years with green manure, one year with corn, one year with Avena strigosa and two years with brachiaria, the following properties were analyzed: pH, Ca, Mg, P, K, cation exchange capacity, base saturation, and organic matter. The treatments succeeded in recovering the soil chemical properties. Stizolobium aterrimum performed better than Cajanus cajan and Canavalia ensiformis. Soil recovery effects reached a depth of 0.0-0.2 m. The recovery techniques (green manure, lime and gypsum improved soil chemical characteristics to a of 0.0-0.1 m in the first year and only after five years the depth of 0.0-0.2 m.

  19. Biological fixation and nitrogen transfer by three legume species in mango and soursop organic orchards;Fixacao biologica e transferencia de nitrogenio por leguminosas em pomar organico de mangueira e gravioleira

    Energy Technology Data Exchange (ETDEWEB)

    Paulino, Gleicia Miranda; Barroso, Deborah Guerra, E-mail: gleiciamiranda@yahoo.com.b, E-mail: deborah@uenf.b [Universidade Estadual do Norte Fluminense (UENF), Campos dos Goytacazes, RJ (Brazil). Dept. de Fitotecnia; Alves, Bruno Jose Rodrigues; Urquiaga, Segundo; Espindola, Jose Antonio Azevedo, E-mail: bruno@cnpab.embrapa.b, E-mail: urquiaga@cnpab.embrapa.b, E-mail: jose@cnpab.embrapa.b [EMBRAPA Agrobiologia, Seropedica, RJ (Brazil)

    2009-12-15

    The objective of this work was to evaluate the biological nitrogen fixation (BNF) and the N transfer derived from BNF of the legume species - Gliricidia sepium (gliricidia), Crotalaria juncea (sunnhemp) and Cajanus cajan (pigeon pea) - for an intercropped organic orchard with mango and soursop, through the {sup 15}N natural abundance method. The following inter cropping systems were evaluated: mango and soursop with gliricidia; mango and soursop with sunnhemp; mango and soursop with pigeon pea; and mango and soursop as control. Gliricidia showed the highest BNF potential (80%) , followed by sunnhemp (64.5%) and pigeon pea (45%). After two sunnhemp prunes, 149.5 kg ha{sup -1} of N per year were supplied, with 96.5 kg derived from BNF. After three annual prunes, gliricidia supplied 56.4 and 80.3 kg ha{sup -1} of N per year, with 45 and 64 kg derived from BNF, in two consecutive years. The quantity of N supplied to the system was higher than the mango and soursop requirements. Variations in the natural abundance of {sup 15}N were found only in soursop leaves. Gliricidia and sunnhemp were prominent in N transfer, with approximately 22.5 and 40% respectively. Green manuring using gliricidia permits fractioning of the N supply, which is an advantage in N obtention by the fruit trees (author)

  20. DEVELOPMENT AND PERFORMANCE EVALUATION OF TRACTOR FRONT MOUNTED PIGEON PEA STEM CUTTER

    Directory of Open Access Journals (Sweden)

    Atul R. Dange

    2010-07-01

    Full Text Available Pigeon pea or tur (Cajanus cajan L. Mills. is one of the important pulse crops of India and ranks second to chickpea in area and production. Traditionally the harvesting of pigeon pea is done manually by sickle, which demands considerable amount of labour, drudgery, time and cost to harvest, which reflects on total production cost of the crop. In view of this a tractor operated front mounted pigeon pea stem cutter was developed and being front mounted implement it facilitated better visibility and control to operator. The power was transmitted from pto to gear box. Arrangement of hydraulic cylinder and hydraulic motor was provided on the equipment to facilitate the height of cut and to rotate the conveyer belt. During comparative performance evaluation of developed equipment, the average cutting efficiency and field capacity was found 96.30 % and 0.176 ha/hr respectively. There was increase in fuel consumption and plant damage with increase in speed of operation. The average operation cost of newly developed tractor operated front mounted pigeon pea stem cutter was 64.71% less as compared with manual harvesting of pigeon pea crop. The time saved was almost 1/3rd to that of manual harvesting.

  1. Propriedades do solo e sistema radicular do feijoeiro irrigado em rotação de culturas

    Directory of Open Access Journals (Sweden)

    E. B. Wutke

    2000-09-01

    Full Text Available O crescimento do sistema radicular do feijoeiro irrigado, cultivar IAC-Carioca, sob pivô central, em rotação com pousio, milho, aveia preta, Crotalaria juncea L., guandu (Cajanus cajan (L. Millsp. e mucuna preta (Mucuna aterrima, no período não-convencional de cultivo no outono-inverno, foi avaliado de 1993 a 1995, em Latossolo Vermelho eutroférrico típico, em área experimental do Instituto Agronômico, em Ribeirão Preto, São Paulo. A rotação de culturas do feijoeiro com milho e adubos verdes favoreceu a redução da resistência do solo à penetração na camada arável, garantiu a manutenção do teor de matéria orgânica do solo, bem como possibilitou a redução da acidez e o aumento do índice de saturação por bases (V% em profundidade em relação ao teor inicial. A velocidade de infiltração básica da água no solo foi favorecida pela inclusão da mucuna preta, da Crotalaria juncea L. e do milho, no esquema de rotações. A profundidade efetiva observada do seu sistema radicular foi de 0,35 a 0,40 m.

  2. Isoflavones hydrolisis and extraction

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    Jozilene Fernandes Farias dos Santos

    2012-12-01

    Full Text Available Isoflavones are found in leguminous species and are used as phytoestrogens widely used by industry for its beneficial effects as estrogens mimicked, antioxidant action and anti-cancer activity. The identification and quantification of isoflavones in plants is a need due to the high demand of industry. Several methods are used for its extraction, using organic solvents (methanol, ethanol and acetonitrile. Samples from five legumes species from Instituto de Zootecnia (IZ, Forage Gene Bank were tested. All seeds received a hydrothermic treatment immersed in pure water at 50°C for 12 hours. Seeds were then oven-dryed. In this work we tested the extraction using only the hydrothermic treatment and hyfrothermic treatment allied to methanol extaction protocol. Seeds were grinded and half of the samples were ressuspended in PBS (phosphate Buffer and the other half were submited to 4 mL of methanol and 1% of acetic acid, soaked for 5 hours, shaked every 15 minutes, at room temperature. The five legume species that we quantify isoflavones by enzyme immunoassay (EIA were: Calopogonium mucunoides, Bauhinia sp., Cajanus cajan, Galactia martii, Leucaena leucocephala. The extraction procedure is a recomendation of AOAC (Association of Official Analytical Chemists for isoflavone quantification. Ours results show an increase of extraction using methanol 80% plus acetic acid 1% and was obtained using solvent extraction in comparison to hydrothermic procedure alone (figure 1.

  3. Phenology of Migration and Decline in Colony Numbers and Crop Hosts of Giant Honeybee (Apis dorsata F. in Semiarid Environment of Northwest India

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    Ram Chander Sihag

    2014-01-01

    Full Text Available The colonies of the giant honeybee (Apis dorsata immigrate in the semiarid environment of Northwest India in October-November with the onset of flowering on pigeon pea (Cajanus cajan/toria (Brassica campestris var. toria, stay here during the rich pollen and nectar flow period from December to mid-May, and emigrate in late May/early June when floral dearth is witnessed. This honeybee was free from any conspicuous viral, bacterial, and fungal diseases and also did not have any serious predators and enemies. However, about 20 percent of the old colonies were infested with Tropilaelaps clareae and 100 percent of the old colonies with Galleria mellonella; none of the swarm colonies had these pests. While the migration schedule of this honeybee remained similar year after year, the number of colonies immigrating in this region declined markedly over the years; the number in 2012 was even less than half of that recorded in 1984. During its stay in this region, this honeybee acted as an important pollinator of more than 30 crop plants of this region. The causes of seasonal migration and decline in the number of colonies of this honeybee and its importance in crop pollination have been discussed.

  4. Productive performance of naked neck chickens that were fed leaf meal shrubs

    Directory of Open Access Journals (Sweden)

    Santos M Herrera G.

    2015-12-01

    Full Text Available Objective. To compare the productive performance of naked neck chickens (phases of initiation, growth and final that were fed meals Gliricidia sepium, Cajanus cajan and Morus alba leaves. Materials and methods. 192 chickens, 1-84 days of age were distributed in a randomized block design with three experimental groups (5% of shrub in the diet, 48 animals/ group, eight replicates/ treatment, six animals/ reply and three animals/ sex in each replicate were used. The control group consumed diet based on corn and soybeans. They were reared on floor. Weighed every seven days. Weight gain, voluntary intake, conversion, balance and efficiency of feed utilization were calculated. Results. The highest total feed intake and average daily gain in rearing were 37.43g 9509.96 g respectively for M. alba (p<0.05, which also presented the best efficiency of energy and protein. Meanwhile, G. sepium showed the lowest values. Conclusions. It is possible to replace 5% of corn and soy in the diet of naked neck chickens, with the inclusion of leaf meal M. alba and get a favorable productive behavior.

  5. Analysis of genetic diversity in pigeon pea germplasm using retrotransposon-based molecular markers

    Indian Academy of Sciences (India)

    MANEESHA; KAILASH C. UPADHYAYA

    2017-09-01

    Pigeon pea (Cajanus cajan), an important legume crop is predominantly cultivated in tropical and subtropical regions of Asia and Africa. It is normally considered to have a low degree of genetic diversity, an impediment in undertaking crop improvement programmes.We have analysed genetic polymorphism of domesticated pigeon pea germplasm (47 accessions) across the world using earlier characterized panzee retrotransposon-based molecularmarkers. Itwas conjectured that since retrotransposons are interspersed throughout the genome, retroelements-based markers would be able to uncover polymorphism possibly inherent in the diversity of retroelement sequences. Two PCR-based techniques, sequence-specific amplified polymorphism (SSAP) and retrotransposon microsatellite amplified polymorphism (REMAP) were utilized for the analyses.We show that a considerable degree of polymorphism could be detected using these techniques. Three primer combinations in SSAP generated 297 amplified products across 47 accessionswith an average of 99 amplicons per assay. Degree of polymorphism varied from 84–95%. In the REMAP assays, the number of amplicons was much less but up to 73% polymorphism could be detected. On the basis of similarity coefficients, dendrograms were constructed. The results demonstrate that the retrotransposon-based markers could serve as a better alternative for the assessment of genetic diversity in crops with apparent low genetic base.

  6. Índice de priorização para avaliar a contenção vegetativa em talude rodoviário de saprolito de gnaisse, na Zona da Mata de Minas Gerais Priorization index to evaluate vegetative containment of a gnaisse saprolite roadcut in the Zona da Mata region, Minas Gerais State

    Directory of Open Access Journals (Sweden)

    Rosilene Einloft

    2009-01-01

    Full Text Available O objetivo desse trabalho foi formular um índice de priorização para avaliar a contenção vegetativa em talude rodoviário de declividade acentuada subtrecho entre Viçosa e Araponga (MG, Brasil, a 20º44' latitude S; 42º50' longitude W. Neste trabalho, os tratamentos consistiram de nove consórcios de uma gramínea (Brachiaria brizantha, Brachiaria decumbens ou Brachiaria ruziziensis e uma leguminosa [mucuna preta (Stizolobium aterrimum, feijão guandu (Cajanus cajan ou lablabe (Dolichos lab lab]. Na elaboração do índice, o primeiro passo foi escolher, dentre as características analisadas, aquelas mais apropriadas para a seleção do consórcio no processo de revegetação estabelecendo, consequentemente, uma frequência por meio de uma matriz de ponderação. Ao término do exercício, a frequência com que uma característica foi assinalada, representa a importância individual na resposta global do consórcio aos tratamentos. A nota final de cada consórcio foi dada pelo somatório do produto das frequências multiplicadas pelos valores relativos das características determinadas experimentalmente. Após o cálculo, os índices foram analisados estatisticamente, aplicando-se o teste de agrupamento de Scott-Knott. Da análise do índice conjunto padronizado, pode-se concluir que a mucuna-preta foi a leguminosa mais apropriada no processo de revegetação, dentre as três testadas, com valor médio de 28,03 sobre máximo possível de 30. O feijão-guandu revelou comportamento intermediário (26,52 e o lab lab foi o menos indicado (23,08. As características semelhantes de crescimento das braquiárias permitiram atestar respostas alternativas, dependendo da leguminosa associada. Assim, a Braquiaria brizanta foi a mais adequada para associação com a mucuna-preta, e a Braquiaria decumbens, quando em consórcio com o feijão-guandu.The objective of this study was to formulate a prioritization index to evaluate vegetative containment on

  7. Erosão em entressulcos sob caatinga e culturas agrícolas Interrill erosion under shrub and crop systems

    Directory of Open Access Journals (Sweden)

    Francisco José de Freitas

    2008-08-01

    Full Text Available Dentre as formas de erosão hídrica do solo, a erosão em entressulcos é uma das que causam maiores danos no processo produtivo dos solos utilizados na agricultura, por provocar perdas de solo, água e nutrientes. Frente a isso, o presente trabalho visou avaliar a proteção ambiental promovida pela cobertura vegetal da caatinga, do feijão-guandu (Cajanus cajan e da batata-doce (Ipomoea batatas Lam em relação ao solo descoberto, bem como o efeito das práticas de cultivos dessas culturas, por meio das taxas da erosão em entressulcos e das características hidráulicas do escoamento superficial vinculado à erosão em entressulcos, em um Luvissolo. Para isso, foi implementada uma série de 20 chuvas simuladas em Serra Talhada, município do semi-árido do Estado de Pernambuco, sob os seguintes tratamentos: (1 solo descoberto; (2 solo coberto pela cultura do feijão-guandu (Cajanus cajan; (3 solo coberto pela cultura da batata-doce (Ipomoea batatas Lam.; (4 solo coberto por Caatinga semi-arbustiva. Todos os regimes de escoamento superficial obtidos foram laminar lento. As coberturas vegetais proporcionadas pela Caatinga e pelo guandu, que deram origem aos maiores valores de cobertura do solo, responderam pelas menores taxas de concentração de sedimentos e desagregação do solo na erosão em entressulcos, em decorrência da maior rugosidade hidráulica da superfície do solo promovida por essas vegetações. As taxas de concentração de sedimentos no cultivo da batata-doce foram iguais às do solo descoberto, por conta do revolvimento do solo na preparação das leiras de plantio, e determinou as maiores taxas de desagregação do solo entre as coberturas vegetais; em contrapartida, as leiras permitiram retenção do escoamento superficial. As coberturas vegetais Caatinga, guandu e batata-doce e as respectivas rugosidades hidráulicas impostas ao escoamento superficial determinaram reduções exponenciais das perdas de solo. A cobertura

  8. Use of nitrogen from fertilizer and cover crops by upland rice in an Oxisol under no-tillage in the Cerrado

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Edson Cabral da; Muraoka, Takashi; Bendassolli, Jose Alberto, E-mail: edsoncabralsilva@gmail.com, E-mail: muraoka@cena.usp.br, E-mail: jab@cena.usp.br [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil); Franzini, Vinicius Ide, E-mail: vinicius.franzini@embrapa.br [Embrapa Amazonia Oriental, Belem, PA (Brazil); Sakadevan, Karuppan, E-mail: K.Sakadevan@iaea.org [Joint FAO/IAEA, Division of Nuclear Techniques in Food and Agriculture, Soil and Water Management and Crop Nutrition Subprogram, Vienna International Centre, Vienna (Austria); Buzetti, Salatier; Arf, Orivaldo, E-mail: sbuzetti@agr.feis.unesp.br, E-mail: arf@agr.feis.unesp.br [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Ilha Solteira, SP (Brazil). Faculdade de Engenharia; Soares, Frederico Antonio Loureiro, E-mail: fredalsoares@hotmail.com [Instituto Federal Goiano, Rio Verde, GO (Brazil)

    2016-06-15

    The objective of this work was to evaluate the effects of cover crops on the yield of upland rice (Oryza sativa) grown under no-tillage system, in the presence and absence of N fertilizer, as well as to quantify, in the field, the use efficiency of N from urea and cover crops by upland rice, through the {sup 15}N isotope dilution technique. The field experiment was carried out in the municipality of Selviria, in the state of Mato Grosso do Sul, Brazil, in an Oxisol (Rhodic Hapludox) in the Cerrado (Brazilian savanna) region. The experimental design was a randomized complete block with 15 treatments and four replicates, in a 5 x 3 factorial arrangement. The treatments were four cover crops (Crotalaria juncea, Cajanus cajan, Mucuna pruriens, and Pennisetum glaucum) + spontaneous vegetation (fallow in off-season), combined with three forms of N fertilization: control treatment, without N fertilizer application; 20 kg ha{sup -1} N at sowing; and 20 kg ha{sup -1} N at sowing plus 60 kg ha{sup -1} N as topdressing. Rice is not affected by N fertilizer application as topdressing, when legume cover crops are used. The use of legume cover crops provides higher grain yield and use of fertilizer-N by rice than that of millet or fallow. Legume cover crops promote an effect equivalent to that of the application of 60 kg ha{sup -1} N as urea on rice yield. (author)

  9. Identification of lipoxygenase (LOX) genes from legumes and their responses in wild type and cultivated peanut upon Aspergillus flavus infection

    Science.gov (United States)

    Song, Hui; Wang, Pengfei; Li, Changsheng; Han, Suoyi; Lopez-Baltazar, Javier; Zhang, Xinyou; Wang, Xingjun

    2016-01-01

    Lipoxygenase (LOX) genes are widely distributed in plants and play crucial roles in resistance to biotic and abiotic stress. Although they have been characterized in various plants, little is known about the evolution of legume LOX genes. In this study, we identified 122 full-length LOX genes in Arachis duranensis, Arachis ipaënsis, Cajanus cajan, Cicer arietinum, Glycine max, Lotus japonicus and Medicago truncatula. In total, 64 orthologous and 36 paralogous genes were identified. The full-length, polycystin-1, lipoxygenase, alpha-toxin (PLAT) and lipoxygenase domain sequences from orthologous and paralogous genes exhibited a signature of purifying selection. However, purifying selection influenced orthologues more than paralogues, indicating greater functional conservation of orthologues than paralogues. Neutrality and effective number of codons plot results showed that natural selection primarily shapes codon usage, except for C. arietinum, L. japonicas and M. truncatula LOX genes. GCG, ACG, UCG, CGG and CCG codons exhibited low relative synonymous codon usage (RSCU) values, while CCA, GGA, GCU, CUU and GUU had high RSCU values, indicating that the latter codons are strongly preferred. LOX expression patterns differed significantly between wild-type peanut and cultivated peanut infected with Aspergillus flavus, which could explain the divergent disease resistance of wild progenitor and cultivars. PMID:27731413

  10. Composição química de grãos de feijao-guandu cultivar kaki Chemical composition of pigeon pea seeds

    Directory of Open Access Journals (Sweden)

    João Paulo Feijão Teixeira

    1985-01-01

    Full Text Available Foram analisadas sementes de tegumento creme e alaranjado de feijão-guandu (Cajanus cajan (L. Milisp., oriundas do cultivar Kaki, quanto aos teores de proteína, extrato etéreo, açúcares solúveis e polissacarídeos. Os dois tipos de sementes apresentaram composição química similar. Os teores médios encontrados foram: proteína 23,11%, extrato etéreo 1,34%, açúcar solúvel 9,46% e polissacarídeos 16,77%. A fração protéica apresentou baixos níveis de aminoácidos sulfurados, isoleucina, valina e treonina.Pigeon pea seeds, cv' Kaki either with light-yellow or orange testa pigments were analysed for protein, ether extract, total sugar and polysaccharides. Similar results were obtained for the two types of seeds. On the average the seeds had the following contents: protein - 23.11%; ether extract - 1.34%; total sugar - 9.46%; polysaccharides - 16.77%. The protein fraction had low levels of sulfured aminoacids, isoleucine, valine and threonine.

  11. Agro-forestry practices and sustainable agriculture in yam producing communities of Niger state, Nigeria

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    Alamu L.O.

    2013-07-01

    Full Text Available In recognition of farmland burden in terms of land degradation, bulky nature of and unavailability of inorganic fertilizers as well as time constraint in the formation of organic fertilizers, there is the need to shift to agro-forestry practices. The practice will make room for arable crop production and forest/tree crop production. Four autonomous communities were studied in Niger State Nigeria. The communities are generally known for yam production. One hundred and twenty farmers were randomly selected for the study in Koro, Gwari, Kadara and Kambari communities. Thirty yam farmers were selected in each community using random sampling technique. One hundred and twenty farmers were studied in all. Results were analysed using simple percentages and pie charts. Results show that there is a high level of consciousness of agro-forestry among the farmers. Shrubs such as Gliricidia sepium and Cajanus cajan are common and they make a cumulative percentage of 35.0% of tree species used for agro- forestry practices. Fruit trees such as Citrus species make a cumulative percentage of 31.0% while other tree species used includes but not exclusive to Tectona grandis, Gmelina arborea and Terminalia species. It is recommended that youths and young farmers in the communities be more involved in agro-forestry practices of this nature for a sustainable practice to be ensured.

  12. OS SISTEMAS AGROFLORESTAIS COMO ALTERNATIVA ECONÔMICA EM PEQUENAS PROPRIEDADES RURAIS: ESTUDO DE CASO

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    Mário Jorge Campos dos Santos

    2002-05-01

    Full Text Available Os Sistemas Agroflorestais (SAFs constituem-se em uma alternativa de uso da terra para aliar a estabilidade do ecossistema visando à eficiência e otimização de recursos naturais na produção de forma integrada e sustentada. O objetivo deste estudo de caso foi avaliar a viabilidade econômica de um sistema agroflorestal localizado na região do Pontal do Paranapanema. O SAF em estudo é formada por espécies agrícolas: milho (Zea maiz, feijão guandu (Cajanus cajan, carioquinha, (Vigna unguiculata, preto (Phaseolus vulgaris e mandioca (Manihot esculenta com espécies florestais: Eucalyptus citriodora e Eucalyptus camaldulensis. A rentabilidade econômica do sistema foi mensurada, utilizando Valor Presente Líquido (VPL, Razão Benefício/Custo (RB/C para as culturas agrícolas anuais e Valor Esperado da terra (VET para os componentes madeireiros. Para tais cálculos foi escolhida uma taxa de desconto de 6%. O estudo demonstrou que o sistema agroflorestal apresentou rentabilidade econômica positiva, e que podem ser adotados por pequenos produtores. Assim, pode-se concluir que os sistemas agroflorestais são economicamente viáveis para pequenos produtores rurais trabalhando nas mesmas condições apresentadas neste estudo.

  13. Diversity of root nodule bacteria from leguminous crops

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    Agrawal Pooja

    2016-01-01

    Full Text Available In the present study, a total of 353 nodule-associated bacteria were isolated from 220 legume plant samples belonging to Cicer arietinum (85, Glycine max (74, Vigna radiata (21 and Cajanus cajan (40. A total of 224 bacteria were identified as fast-growing Rhizobium spp. on the basis of differential staining (Gram staining and carbol fuchsin staining and biochemical tests. All the isolates were tested for indole acetic acid production (IAA, phosphate solubilization and siderophore production on plate assay. To examine the effect of volatile organic metabolites (VOM and water soluble soil components (WSSC on nodule bacteria, culture conditions were optimized by observing the effects of various parameters such as pH, salt content and temperatures on the growth of bacteria. Selected rhizobia were subjected to random amplified polymorphic DNA (RAPD and amplified ribosomal DNA restriction analysis (ARDRA analysis to identify their species. On the basis of RAPD and ARDRA, 10 isolates were identified as Rhizobium meliloti. In this study, Rhizobium GO4, G16, G20, G77, S43, S81, M07, M37, A15 and A55 were observed as the best candidates among the tested bacteria and can be further used as potent bioinoculants.

  14. Two rhizobacterial strains, individually and in interactions with Rhizobium sp., enhance fusarial wilt control, growth, and yield in pigeon pea.

    Science.gov (United States)

    Dutta, Swarnalee; Morang, Pranjal; Kumar S, Nishanth; Dileep Kumar, B S

    2014-09-01

    A Pseudomonas aeruginosa strain, RRLJ 04, and a Bacillus cereus strain, BS 03, were tested both individually and in combination with a Rhizobium strain, RH 2, for their ability to enhance plant growth and nodulation in pigeon pea (Cajanus cajan L.) under gnotobiotic, greenhouse and field conditions. Both of the rhizobacterial strains exhibited a positive effect on growth in terms of shoot height, root length, fresh and dry weight, nodulation and yield over the non-treated control. Co-inoculation of seeds with these strains and Rhizobium RH 2 also reduced the number of wilted plants, when grown in soil infested with Fusarium udum. Gnotobiotic studies confirmed that the suppression of wilt disease was due to the presence of the respective PGPR strains. Seed bacterization with drug-marked mutants of RRLJ 04 and BS 03 confirmed their ability to colonize and multiply along the roots. The results suggest that co-inoculation of these strains with Rhizobium strain RH 2 can be further exploited for enhanced growth, nodulation and yield in addition to control of fusarial wilt in pigeon pea.

  15. Green manure and its influence on bean (Phaseolus vulgaris L. in agroecological system

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    Marisol Rivero Herrada

    2016-07-01

    Full Text Available Green manures have been used as biomass producers and suppliers of nutrients and keep the soil productive potential in tropical regions. The objective of this study was to evaluate the production of dry biomass and nutrient concentration and accumulation in plants of green manure in two cropping systems, without associating and associated with millet and its influence on the nutritional status of the bean (Phaseolus vulgaris L. in succession in agroecological production. Four legumes (Canavalia ensiformis Adans; Cajanus cajan (L. Millsp.; Crotalaria juncea L.; Mucuna pruriens (L. DC and one grass (Pennisetum glaucum L. as association plant, were evaluated. A randomized block design was used, with eight treatments and four repetitions. The green manure plants were cut and left on the ground at 60 days after planting and bean was planted 20 days after cutting the manure plants. The evaluated variables were dried biomass production, nutrient content of green manure and nutrient content in leaves of beans in succession. The production of dry biomass of green manure was higher than 9.00 t ha-1. Mucuna stood out with the greatest tenors of N, Ca, Mg, pork beans with the highest tenors of K, Cu, Mn. The greatest accumulation of P, K and Ca nutrients was in pigeon pea. The highest values of N and Mg were obtained in mucuna. Higher C / N relation was obtained in rotalaria.

  16. Allelopathic effects of leaf litters of Eucalyptus camaldulensis on some forest and agricultural crops

    Institute of Scientific and Technical Information of China (English)

    Romel Ahmed; A. T. M. Rafiqul Hoque; Mohammed Kamal Hossain

    2008-01-01

    Allelopathic effects of different doses of Eucalyptus camaldulensis leaf litters were investigated through an experiment in the green house of Institute of Forestry and Environmental Sciences, Chittagong, Bangladesh. Three popular agricultural crops: Falen (Vigna unguiculata), Chickpea (Cicer arietinum), Arhor (Cajanus cajan) and two widely used plantation trees: Sada koroi (Albizia procera) and Ipil ipil (Leucaena leucocephala) were selected as bioassay species. Experiment was set on tray at room temperature 27℃. The effects of different doses of leaf litter extracts were compared to the control. Results suggest that leaf litters of E. camaldulesis induced inhibitory effects. It was also found that the effect depend on concentration of extract and litterfall, type of receiver species. Higher concentration of the materials had the higher effect and vice versa. Though all the bioassay species were suppressed some of them showed better performance. Vigna unguiculata, Cicer arietinum are recommended in agroforestry based on this present Experiment output. In mixed plantation, Leucaena leucochephala is a better choice while compared to Albizia procera.

  17. Allelopathic effects of Leucaena leucocephala leaf litter on some forest and agricultural crops grown in nursery

    Institute of Scientific and Technical Information of China (English)

    Romel Ahmed; A. T. M. Rafiqul Hoque; Mohammed Kamal Hossain

    2008-01-01

    An experiment was conducted to assess the effect of leaf litter of Leucaena leucocephala on two forest crops Sada koroi (Albizia procera),Ipil ipil (L.leucocephala) and three agricultural crops Falen (Vigna unguiculata),Chickpea (Cicer arietinum) and Arhor (Cajanus cajan) in the nursery of the Institute of Forestry and Environmental Sciences,Chittagong University,Bangladesh,in a Randomized Block Design.Results suggested that leaf litters of L.leucocephala induced inhibitory effects on germination and growth of bioassay.It was also found that the effect depended on concentration of extract and litterfall,type of receptor species.Higher concentration of the materials had the higher effect and vice versa.Growth response of receptor crops varied with the variation of leaf litter application.The study revealed that application of low-dose leaf litter specially litter of 10 g(m-2 had stimulating effect on shoot growth of C.arietinum,V.unguiculata and A.procera.While in all other cases significant inhibitory effect was observed and it was significantly increased with the increase of leaf litter application.However,the trend of inhibition was uneven with treatments.Root growth was found to be more affected than shoot growth.

  18. Plantio direto de alface americana sobre plantas de cobertura dessecadas ou roçadas

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    Andréia Cristina Silva Hirata

    2014-06-01

    Full Text Available O objetivo deste trabalho foi avaliar o plantio direto de alface americana no verão sobre plantas de cobertura dessecadas ou roçadas em cultivos sucessivos. O trabalho foi desenvolvido no município de Álvares Machado, São Paulo, Brasil. O delineamento experimental foi em blocos ao acaso, dispostos em parcelas subdivididas, com quatro repetições. O fator da parcela principal foram dois manejos das plantas de cobertura (dessecadas com herbicida ou roçadas e as subparcelas, seis espécies de plantas de cobertura (Cajanus cajan cv. IAPAR 43, Crotalaria spectabilis, Crotalaria juncea, Mucuna pruriens, Pennisetum glaucum e vegetação natural, além da testemunha sem plantas de cobertura. O manejo roçado apresentou desempenho inferior no primeiro cultivo da alface, todavia não diferiu do manejo químico no segundo cultivo. A cobertura do solo com mucuna apresentou maior produtividade da alface no primeiro cultivo devido ao excesso de palha das demais coberturas, o qual prejudicou o estabelecimento da alface. No segundo cultivo não houve diferenças entre as coberturas. A roçagem de plantas de cobertura é uma opção viável para plantio direto de alface sem herbicidas.

  19. Siderophore as a potential plant growth-promoting agent produced by Pseudomonas aeruginosa JAS-25.

    Science.gov (United States)

    Sulochana, M B; Jayachandra, S Y; Kumar, S Anil; Parameshwar, A B; Reddy, K Mohan; Dayanand, A

    2014-09-01

    Siderophores scavenges Fe(+3) from the vicinity of the roots of plants, and thus limit the amount of iron required for the growth of pathogens such as Fusarium oxysporum, Pythium ultimum, and Fusarium udum, which cause wilt and root rot disease in crops. The ability of Pseudomonas to grow and to produce siderophore depends upon the iron content, pH, and temperature. Maximum yield of siderophore of 130 μM was observed at pH 7.0 ± 0.2 and temperature of 30 °C at 30 h. The threshold level of iron was 50 μM, which increases up to 150 μM, favoring growth but drastically affecting the production of siderophore by Pseudomonas aeruginosa JAS-25. The seeds of agricultural crops like Cicer arietinum (chick pea), Cajanus cajan (pigeon pea), and Arachis hypogaea (ground nut) were treated with P. aeruginosa JAS-25, which enhanced the seed germination, root length, shoot length, and dry weight of chick pea, pigeon pea, and ground nut plants under pot studies. The efficient growth of the plants was not only due to the biocontrol activity of the siderophore produced by P. aeruginosa JAS-25 but also may be by the production of indole acetic acid (IAA), which influences the growth of the plants as phytohormones.

  20. Os sistemas agroflorestais como alternativa econômica em pequenas propriedades rurais: estudo de caso.

    Directory of Open Access Journals (Sweden)

    Mário Jorge Campos dos Santos

    2010-08-01

    Full Text Available Os Sistemas Agroflorestais (SAFs constituem-se em uma alternativa de uso da terra para aliar a estabilidade do ecossistema visando à eficiência e otimização de recursos naturais na produção de forma integrada e sustentada. O objetivo deste estudo de caso foi avaliar a viabilidade econômica de um sistema agroflorestal localizado na região do Pontal do Paranapanema. O SAF em estudo é formada por espécies agrícolas: milho (Zea maiz, feijão guandu (Cajanus cajan, carioquinha, (Vigna unguiculata, preto (Phaseolus vulgaris e mandioca (Manihot esculenta com espécies florestais: Eucalyptus citriodora e Eucalyptus camaldulensis. A rentabilidade econômica do sistema foi mensurada, utilizando Valor Presente Líquido (VPL, Razão Benefício/Custo (RB/C para as culturas agrícolas anuais e Valor Esperado da terra (VET para os componentes madeireiros. Para tais cálculos foi escolhida uma taxa de desconto de 6%. O estudo demonstrou que o sistema agroflorestal apresentou rentabilidade econômica positiva, e que podem ser adotados por pequenos produtores. Assim, pode-se concluir que os sistemas agroflorestais são economicamente viáveis para pequenos produtores rurais trabalhando nas mesmas condições apresentadas neste estudo.

  1. Study the effect of insecticide dimethoate on photosynthetic pigments and photosynthetic activity of pigeon pea: Laser-induced chlorophyll fluorescence spectroscopy.

    Science.gov (United States)

    Pandey, Jitendra Kumar; Dubey, Gunjan; Gopal, R

    2015-10-01

    Pigeon pea is one of the most important legume crops in India and dimethoate is a widely used insecticide in various crop plants. We studied the effect of dimethoate on growth and photosynthetic activity of pigeon pea plants over a short and long term exposure. Plant growth parameters, photosynthetic pigment content and chlorophyll fluorescence response of pigeon pea (Cajanus cajan L.) plants treated with various concentrations of the insecticide dimethoate (10, 20, 40 and 80 ppm) have been compared for 30 days at regular intervals of 10 days each. Laser induced chlorophyll fluorescence spectra and fluorescence-induction kinetics (FIK) curve of dimethoate treated pigeon pea plants were recorded after 10, 20 and 30 days of treatment. Fluorescence intensity ratio at the two fluorescence maxima (F685/F730) was calculated by evaluating curve-fitted parameters. The variable chlorophyll fluorescence decrease ratio (Rfd) was determined from the FIK curves. Our study revealed that after 10 days of treatment, 10 ppm of dimethoate showed stimulatory response whereas 20, 40 and 80 ppm of dimethoate showed inhibitory response for growth and photosynthetic activity of pigeon pea plants, but after 20 and 30 days of treatment all the tested concentrations of dimethoate became inhibitory. This study clearly shows that dimethoate is highly toxic to the pigeon pea plant, even at very low concentration (10 ppm), if used for a prolonged duration. Our study may thus be helpful in determining the optimal dose of dimethoate in agricultural practices.

  2. LegumeIP 2.0--a platform for the study of gene function and genome evolution in legumes.

    Science.gov (United States)

    Li, Jun; Dai, Xinbin; Zhuang, Zhaohong; Zhao, Patrick X

    2016-01-04

    The LegumeIP 2.0 database hosts large-scale genomics and transcriptomics data and provides integrative bioinformatics tools for the study of gene function and evolution in legumes. Our recent updates in LegumeIP 2.0 include gene and protein sequences, gene models and annotations, syntenic regions, protein families and phylogenetic trees for six legume species: Medicago truncatula, Glycine max (soybean), Lotus japonicus, Phaseolus vulgaris (common bean), Cicer arietinum (chickpea) and Cajanus cajan (pigeon pea) and two outgroup reference species: Arabidopsis thaliana and Poplar trichocarpa. Moreover, the LegumeIP 2.0 features the following new data resources and bioinformatics tools: (i) an integrative gene expression atlas for four model legumes that include 550 array hybridizations from M. truncatula, 962 gene expression profiles of G. max, 276 array hybridizations from L. japonicas and 56 RNA-Seq-based gene expression profiles for C. arietinum. These datasets were manually curated and hierarchically organized based on Experimental Ontology and Plant Ontology so that users can browse, search, and retrieve data for their selected experiments. (ii) New functions/analytical tools to query, mine and visualize large-scale gene sequences, annotations and transcriptome profiles. Users may select a subset of expression experiments and visualize and compare expression profiles for multiple genes. The LegumeIP 2.0 database is freely available to the public at http://plantgrn.noble.org/LegumeIP/.

  3. ACUMULAÇÃO DE NUTRIENTES NO LIMBO FOLIAR DE GUANDU E ESTILOSANTES NUTRIENT ACCUMULATION IN PIGEON PEA AND STYLO LEAF BLADE

    Directory of Open Access Journals (Sweden)

    Huberto José Kliemann

    2007-09-01

    Full Text Available

    Guandu (Cajanus cajan e estilosantes (Stylosanthes guianensis var. vulgaris cv. Mineirão são duas leguminosas cultivadas em solos dos cerrados da região Centro-Oeste, utilizadas para adubação verde, produção de sementes e pastoreio. O objetivo deste trabalho foi avaliar a acumulação dos nutrientes N, P, K, Ca, Mg, Zn, Cu, Mn e Fe no limbo foliar dessas leguminosas, em função dos dias após emergência (DAE da planta. O experimento foi conduzido em Latossolo Vermelho distrófico, na Embrapa Arroz e Feijão, em Santo Antônio de Goiás, GO. Os tratamentos foram distribuídos em blocos ao acaso, com quatro repetições. As parcelas tiveram 6,0 m de largura e 20,0 m de comprimento. A adubação de plantio foi de 400 kg ha-1 da fórmula comercial 5-30-15. O plantio foi realizado em dezembro de 2001. Durante o ciclo das culturas, foram tomadas nove amostras aleatórias de limbos foliares para análise foliar. De posse da massa da matéria seca e da concentração dos nutrientes, calcularam-se as suas acumulações no limbo foliar das duas leguminosas. Fez-se o ajuste dos dados de acumulação do nutriente (Y em função dos dias após a emergência (X por um modelo exponencial quadrático Y = a exp(bx + cx2. O guandu produziu mais matéria seca e, de modo geral, teve maior acumulação de nutrientes que o estilosantes, até 98 dias de idade. Dentre os macronutrientes, as maiores acumulações foram de N e as menores de P. O micronutriente de maior acumulação no limbo foliar das duas leguminosas foi Fe, e o de menor acumulação, Cu.

    PALAVRAS-CHAVE: Leguminosas; conteúdo de nutrientes; nutrição mineral; Cajanus cajan; Stylosanthes guyanensis.

  4. Soil Organic Matter Quality of an Oxisol Affected by Plant Residues and Crop Sequence under No-Tillage

    Science.gov (United States)

    Cora, Jose; Marcelo, Adolfo

    2013-04-01

    Plant residues are considered the primarily resource for soil organic matter (SOM) formation and the amounts and properties of plant litter are important controlling factors for the SOM quality. We determined the amounts, quality and decomposition rate of plant residues and the effects of summer and winter crop sequences on soil organic C (TOC) content, both particulate organic C (POC) and mineral-associated organic C (MOC) pools and humic substances in a Brazilian Rhodic Eutrudox soil under a no-tillage system. The organic C analysis in specifics pools used in this study was effective and should be adopted in tropical climates to evaluate the soil quality and the sustainability of various cropping systems. Continuous growth of soybean (Glycine max L. Merrill) on summer provided higher contents of soil POC and continuous growth of maize (Zea mays L.) provided higher soil humic acid and MOC contents. Summer soybean-maize rotation provided the higher plant diversity, which likely improved the soil microbial activity and the soil organic C consumption. The winter sunn hemp (Crotalaria juncea L.), pigeon pea (Cajanus cajan (L.) Millsp), oilseed radish (Raphanus sativus L.) and pearl millet (Pennisetum americanum (L.) Leeke) enhanced the soil MOC, a finding that is attributable to the higher N content of the crop residue. Sunn hemp and pigeon pea provided the higher soil POC content. Sunn hemp showed better performance and positive effects on the SOM quality, making it a suitable winter crop choice for tropical conditions with a warm and dry winter.

  5. Late Embryogenesis Abundant (LEA proteins in legumes

    Directory of Open Access Journals (Sweden)

    Marina eBattaglia

    2013-06-01

    Full Text Available Plants are exposed to different external conditions that affect growth, development, and productivity. Water deficit is one of these adverse conditions caused by drought, salinity, and extreme temperatures. Plants have developed different responses to prevent, ameliorate or repair the damage inflicted by these stressful environments. One of these responses is the activation of a set of genes encoding a group of hydrophilic proteins that typically accumulate to high levels during seed dehydration, at the last stage of embryogenesis, hence named Late Embryogenesis Abundant (LEA proteins. LEA proteins also accumulate in response to water limitation in vegetative tissues, and have been classified in seven groups based on their amino acid sequence similarity and on the presence of distinctive conserved motifs. These proteins are widely distributed in the plant kingdom, from ferns to angiosperms, suggesting a relevant role in the plant response to this unfavorable environmental condition. In this review, we analyzed the LEA proteins from those legumes whose complete genomes have been sequenced such as Phaseolus vulgaris, Glycine max, Medicago truncatula, Lotus japonicus, Cajanus cajan and Cicer arietinum. Considering their distinctive motifs, LEA proteins from the different groups were identified, and their sequence analysis allowed the recognition of novel legume specific motifs. Moreover, we compile their transcript accumulation patterns based on publicly available data. In spite of the limited information on these proteins in legumes, the analysis and data compiled here confirms the high correlation between their accumulation and water deficit, reinforcing their functional relevance under this detrimental conditions.

  6. Study on Plant Species Composition for Revegetatation at Zinc and Indium Mining Area%锌铟矿区植被结构优化造林模式的研究

    Institute of Scientific and Technical Information of China (English)

    常恩福; 侬时增; 刘永国; 李娅; 钱兴廷; 安康发; 周文华

    2013-01-01

    Silvicultural experiments including 10 species composition of 12 tested plant species were conducted in Zinc and indium mine waste accumulation site located in Dulong of Maguan county of Yunnan province from 2010 to 2013.Twelve tested plant species were Cupressus lusitanica,Alnus nepalensis,A.cremastogyne cv.yanshan,Ficus microcarpa vat.pusillifolia,Celtis kunmingensis,Pinus eltiottii,Buxus sinica,Ligustrum quihoui,Cajanus cajan,Neyraudia reynaudiana,Festuca arundinacea and Lolium perenne.Three years after field planting,the growth increments of 10 designed species composition were measured,and the soil nutritional status were analyzed as well.By considering the ecological status,site characteristics and the objectives of revegetation,four plant species with promising adaptability,afforestation survival rate and preserving rate were screened out,they were A.cremastogyne cv.yanshan,Cajanus cajan,A.nepalensis and Neyraudia reynaudiana.Combining A.cremastogyne cv.yanshan with Neyraudia reynaudiana or Cajanus cajan with Neyraudia reynaudiana,could speed up the positive succession,and form multilayer stand structure,with the survival rate over 95 % and preserving rate over 87 %.The average tree height and DBH of three years A.cremastogyne cv.yanshan were 4.79 m and 4.5 cm respectively,the average tree height and DBH of three years Cajanus cajan were 2.16 m and 2.8 cm respectively,the average height of Neyraudia reynaudiana was 2.18 m.The experimental results also indicated that at the initial stage of revegetation,choosing proper plant species and combining the species scientifically,the biodiversity could be maintained effectively,and the ideal community structure could be achieved,which therefore quite promising for the improve the soil fertility of the site.%2010 ~2013年在马关都龙曼家寨锌铟矿山采用墨西哥柏、旱冬瓜、川滇桤木、小叶榕、滇朴、湿地松、黄杨、小叶女桢、木豆、类芦、苇状羊茅、黑麦草12

  7. Weed suppression by green manure in an agroecological system

    Directory of Open Access Journals (Sweden)

    Katia Maria Garicoix Recalde

    2015-12-01

    Full Text Available ABSTRACT Green manure promotes efficient suppression of weeds, but green manure species can exhibit distinct behaviors, depending on the environmental conditions. This study aimed to evaluate the potential of soil mulching and weed suppression by spring/summer green manure species grown in the spring/summer season, at different growth stages and after management (cut, for 90 days during the cassava crop cycle. The study was carried out in the 2010/2011 season, in a system managed under agroecological principles. The treatments consisted of different green manure species and arrangements: Crotalaria juncea, Cajanus cajan, Canavalia brasiliensis, Canavalia ensiformis, Pennisetum americanum, Crotalaria juncea and Pennisetum americanum intercropped; Mucuna aterrima, Sorghum bicolor, a mixture of all the green manures in study and a control plot under fallow. The experiment was arranged in a randomized block design with four replications. The evaluations of the soil cover either by the green manures or weeds were performed at 45, 90 and 105 days after the emergence of the green manures. The cassava crop was planted under reduced tillage system at 11 days after the cut of the green manures. The percentage of soil covered by weeds and the dry matter produced were evaluated at 30, 60 and 90 days after planting. The results showed that the green manures had a suppressive effect on weeds during their life cycle, as well as during the first months after its management (cut, composing the mulch.

  8. Tree Species Selection Trial in Mountain Regions with Karst Feature of Yunnan Southeast%滇东南岩溶山区退耕还林适宜造林树种筛选试验

    Institute of Scientific and Technical Information of China (English)

    李军; 李卓新; 谷勇

    2006-01-01

    地处红河流域中段的滇东岩溶山区退耕还林区适宜造林树种的筛选研究,对发展当地经济、保护岩溶生态环境结果具有重大意义.研究表明:印楝(Azadirachta indica)、甜酸角(Tamarindus indica)、墨西哥柏(Cupressus lusitanica)、马鹿花(Puer ariawallichii)、木豆(Cajanus cajan)等树种适宜在海拔500~800 m地带造林;思茅松(Pinus kesiya var.langbianensis)、墨西哥柏、车桑子(Dodonaea viscose)、马鹿花、木豆等树种适宜在海拔1 000~1 500 m的地带造林,冬樱花(Cerasus cerasoides)、圆柏(Sabina chinensis)、板栗(Castanea mdlissima)、杉木(Cunninghamia lanceolata)等树种适宜在海拔1 800~2 000 m的地带造林.冬樱桃、思茅松、墨西哥柏、印楝等采用百日苗容器苗造林,杉木、圆柏、板栗、甜酸角等采用1年生容器大苗造林效果更好.

  9. Feeding and oviposition preference of Phyllophaga cuyabana (Moser) (Coleoptera: Melolonthidae) on several crops

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Lenita J.; Hoffmann-Campo, Clara B. [EMBRAPA Soja, Londrina, PR (Brazil). Centro Nacional de Pesquisa de Soja]. E-mail: lenita@cnpso.embrapa.br; Garcia, Maria A. [Universidade Estadual de Campinas (UNICAMP), Campinas, SP (Brazil). Inst. de Biologia. Dept. de Zoologia; Amaral, Maria L.B. do [Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brasilia, DF (Brazil)

    2007-09-15

    Laboratory and greenhouse experiments were carried out to study food and oviposition preference by Phyllophaga cuyabana (Moser) on different plant species as Cajanus cajan L. (pigeon pea), Crotalaria juncea L. (sun hemp), Crotalaria spectabilis Roth (showy crotalaria), Crotalaria ochroleuca G. Don (slenderleaf rattlebox), Glycine max [L.] Merrill (soybean), Gossypium hirsutum L. (cotton), Helianthus annuus L. (sunflower), Stizolobium aterrimum [Mucuna aterrima] Piper and Tracey (velvetbean) and Zea mays L. (mayze). In no-choice experiments, the number of eggs layed in sunflower, C. juncea and soybean was larger compared to cotton. Despite the fact that the adults did not discriminate among plants, in dual-choice test, the proportion of eggs layed and leaf consumption by P. cuyabana adults in soybean were significantly higher than in C. spectabilis. The larval distribution in the soil was at random in multiple-choice, without any trend of preference, but in dual-choice, when soybean was the control, larvae always preferred to feed on its roots. P. cuyabana adults had preference for more suitable hosts and that could stand their offspring survival. This behaviour can be usefully exploited in an integrated management program for this pest. (author)

  10. Conversion of cassava wastes for biofertilizer production using phosphate solubilizing fungi.

    Science.gov (United States)

    Ogbo, Frank C

    2010-06-01

    Two fungi characterized as Aspergillus fumigatus and Aspergillus niger, isolated from decaying cassava peels were used to convert cassava wastes by the semi-solid fermentation technique to phosphate biofertilizer. The isolates solubilized Ca(3)(PO(4))(2), AlPO(4) and FePO(4) in liquid Pikovskaya medium, a process that was accompanied by acid production. Medium for the SSF fermentation was composed of 1% raw cassava starch and 3% poultry droppings as nutrients and 96% ground (0.5-1.5mm) dried cassava peels as carrier material. During the 14days fermentation, both test organisms increased in biomass in this medium as indicated by increases in phosphatase activity and drop in pH. Ground cassava peels satisfied many properties required of carrier material particularly in respect of the organisms under study. Biofertilizer produced using A. niger significantly (p<.05) improved the growth of pigeon pea [Cajanus cajan (L.) Millsp.] in pot experiments but product made with A. fumigatus did not. Copyright 2009 Elsevier Ltd. All rights reserved.

  11. Indicadores biológicos associados ao ciclo do fósforo em solos de Cerrado sob plantio direto e plantio convencional Soil biological indicators associated to the phosphorus cycle in a Cerrado soil under no-till and conventional tillage systems

    Directory of Open Access Journals (Sweden)

    Roberto Guimarães Carneiro

    2004-07-01

    Full Text Available O objetivo deste trabalho foi avaliar a influência do plantio direto, do preparo convencional do solo e de plantas de cobertura sobre indicadores biológicos associados ao ciclo do fósforo (P. O trabalho foi realizado em três áreas adjacentes, num Latossolo Vermelho-Amarelo, distribuídas da seguinte maneira: área I, dois anos de experimentação, comparando esses sistemas de plantio; área II, seis anos de experimentação e área III, Cerrado nativo. Em julho/1998 e janeiro/1999, avaliaram-se: o P da biomassa, populações de fungos e bactérias totais e solubilizadores de fosfato e a atividade da fosfatase ácida, nas profundidades de 0-5 e de 5-20 cm. A atividade da fosfatase e a ocorrência de fungos e bactérias solubilizadoras nas áreas com plantio direto foram significativamente superiores às das áreas com plantio convencional, na profundidade de 0-5 cm. O tempo de implantação acentuou as diferenças entre plantio direto e convencional quanto aos indicadores avaliados. O P da biomassa diminuiu no tratamento plantio direto com nabo forrageiro (Raphanus sativus da área II. Os fungos solubilizadores aumentaram na presença de guandu (Cajanus cajan e nabo forrageiro, e as bactérias solubilizadoras aumentaram na presença de guandu. O solo sob vegetação nativa apresentou maior atividade da fosfatase em relação ao das áreas cultivadas, evidenciando a importância da mineralização do P orgânico nesse ecossistema.The objective of this work was to evaluate the effects of no-till, conventional tillage and cover crops on biological indicators associated to the P cycle. The work was carried out on three adjacent areas on a Red-Yellow Oxisol: area I, a two-year experiment comparing the two management systems; area II, a six-year experiment, and area III, a native Cerrado vegetation. The soil was sampled at two depths (0-5 cm and 5-20 cm in July/1998 and January/1999. The biological indicators evaluated were microbial biomass P

  12. População microbiana total e solubilizadora de fosfato em solo submetido a diferentes sistemas de cultivo Total microbial and phosphate-solubilizing population in soil submitted to different cultivation systems

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    GIOVANE BARROTI

    2000-10-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito de diferentes espécies de plantas, fontes de fósforo e calagem sobre a população microbiana total e solubilizadora de fosfato. Foram isolados fungos e bactérias capazes de solubilizar hidroxiapatita, proporcionando P solúvel. O experimento utilizado foi em blocos ao acaso com fatorial 3x3x2. E os fatores avaliados foram espécies de plantas (controle, braquiária e guandu, fertilizantes (controle, superfosfato simples e fosfato de rocha, ambos na dose de 400 kg ha-1 de P(20(5 e calagem (com e sem calcário. A população bacteriana cresceu pelo efeito da calagem, e a de fungos aumentou, independentemente da calagem, nas parcelas cultivadas com braquiária e fertilizadas com superfosfato. Foi constatado incremento de biomassa-P microbiana sobre o controle por influência da braquiária (23,9%, do superfosfato (30,9% e da calagem (46,9%. O número de bactérias solubilizadoras foi favorecido pela calagem ou pelo plantio de guandu adubado com fosfato natural ou com braquiária sem adubação. Os fungos solubilizadores aumentaram na ausência de planta ou de adubação e na presença de guandu com fosfato natural. Finalmente, a calagem favoreceumais o crescimento dos fungos solubilizadores, em comparação com o controle, nos tratamentos fosfato natural, braquiária ou guandu.The objective of the present study was to evaluate the effect of different plant species, phosphorus sources, and liming on the total microbial and phosphate-solubilizing population. In order to achieve that, bacteria and fungi capable of solubilizing hydroxyapatite providing available P were isolated. An experiment was carried out in a randomized factorial block design 3x3x2. The factors evaluated were plant species (control, Brachiaria ruziziensis and Cajanus cajan, fertilizers (control, simple superphosphate and rock phosphate, both at the dose of 400 kg ha-1 of P(20(5 and liming (with and without lime. While the bacterial

  13. Fitomassa e relação C/N em consórcios de sorgo e milho com espécies de cobertura Biomass and C/N ratio in intercrops of sorghum and maize with cover crops

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    Paulo Claudeir Gomes da Silva

    2009-11-01

    Full Text Available O objetivo deste trabalho foi quantificar a produtividade de fitomassa, o teor e acúmulo de nitrogênio (N, e a relação carbono/nitrogênio (C/N de monocultivos de sorgo (Sorghum bicolor e milho (Zea mays e de seus consórcios com guandu-anão (Cajanus cajan, crotalária (Crotalaria juncea, tremoço branco (Lupinus albus, girassol (Helianthus annuus e nabo-forrageiro (Raphanus sativus, manejados em diferentes estádios. O experimento foi conduzido de março a julho de 2008, em Argissolo Vermelho distroférrico de textura média, no sistema plantio direto. O delineamento experimental foi o de blocos completos ao acaso, com quatro repetições, e parcelas subdivididas, constituído pelos tratamentos: monocultivos de sorgo e milho e seus respectivos consórcios com guandu-anão, crotalária, girassol, nabo-forrageiro e tremoço branco, nas parcelas; e épocas de corte, aos 60, 90 e 120 dias após a semeadura nas subparcelas. Consórcios de sorgo e milho com outras espécies superaram expressivamente a produtividade de fitomassa de seus monocultivos que ainda acumularam menos N e apresentaram maiores relações C/N na fitomassa. Para aumentar a produtividade de fitomassa, a melhor época de corte é aos 120 dias após a semeadura das culturas de cobertura. O corte aos 90 dias após a semeadura propicia o maior acúmulo de N e as menores relações C/N.The objective of this work was to quantify the biomass production, the content and accumulation of nitrogen (N and the carbon/nitrogen (C/N relation in monocultures of sorghum (Sorghum bicolor and corn (Zea mays, and intercrops with pigeon pea (Cajanus cajan, sun hemp (Crotalaria juncea, white lupine (Lupinus albus, sunflower (Helianthus annuus and turnip forage (Raphanus sativus, managed in different stages. The experiment was carried out from March to July 2008, in a Haplic Acrisol, medium texture, under no-tillage. A randomized complete block design, with four replicates and split plots with the

  14. PLANT SPECIES FOR REVEGETATION OF OVERBURDEN ROCK AND IRON MINE SPOIL OF THE ALEGRIA IRON MINE, MARIANA, MINAS GERAIS STATE, BRAZIL POTENCIALIDADE DE PLANTAS PARA REVEGETAÇÃO DE ESTÉREIS E REJEITO DA MINERAÇÃO DE FERRO DA MINA DE ALEGRIA, MARIANA-MG

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    Gilson Pereira Silva

    2007-09-01

    Full Text Available

    An experiment was conducted to verify the potentiality of some plant species to revegetate areas under the influence of iron mining. Field conditions were simulated laying mining overburden (soil, phyllite and rock over the mine spoil to cultivate the plants. Four plant species and two irrigation frequencies were tested for pile covering and plant tops dry matter production. Plants showed larger dry matter production when substrate was either soil or mixtures in which soil was one of the components. Among the plants utilized, Brachiaria brizantha and Melinis minutiflora produced the larger amounts of dry matter as compared to Cajanus cajan and Panicum maximum. The frequency of irrigation markedly affected plant growth and development. P. maximum was the species with the least decrease n dry matter production with the reduction of irrigation frequency.

    KEY-WORDS: Iron mining revegetation; irrigation; grasses; legumes.

    Foi conduzido, em casa de vegetação, um experimento para verificar a potencialidade de diversas plantas para a revegetação de áreas de mineração de ferro. Nesse sentido, procurou-se simular a condição de campo colocando-se camadas de estéreis da mina (solo, filito e rocha sobre o rejeito da mineração para o cultivo das diversas plantas utilizadas. Testaram-se quatro espécies vegetais para recobrimento da pilha, duas freqüências de irrigação e avaliou-se a produção de matéria seca da parte aérea das plantas. As plantas apresentaram maior produção de matéria seca da parte aérea quando o substrato foi solo ou misturas nas quais o solo aparecia como componente. Dentre as plantas utilizadas, Brachiaria brizantha (brizantão e Melinis minutiflora (capim-gordura produziram maior quantidade de matéria seca da parte aérea do que Cajanus cajan (guandu e

  15. Efeito de pré-cultivos sobre o potencial de inóculo de fungos micorrízicos arbusculares e produção da mandioca Effect of pre-crops on the inoculum potential of arbuscular mycorrhizal fungi and cassava yield

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    Francisco Adriano de Souza

    1999-10-01

    Full Text Available Avaliou-se o efeito de pré-cultivos com adubos verdes sobre o potencial de inóculo de fungos micorrízicos arbusculares e produção de mandioca (Manihot esculenta, Crantz. O experimento foi conduzido no campo experimental da Embrapa-Centro Nacional de Pesquisa de Agrobiologia (CNPAB, em solo Podzólico Vermelho-Amarelo, localizado no Município de Seropédica, RJ, em parcelas experimentais de 30 m², dispostas em delineamento de blocos casualizados com quatro repetições. Os pré-cultivos constaram de Canavalia ensiformis, Mucuna aterrima, Crotalaria juncea, Cajanus cajan e Sorghum bicolor (BR 005 e o tratamento-controle (solo mantido sem plantas. Após um período de 82 dias de crescimento, sobre as mesmas parcelas cultivou-se mandioca, por oito meses. A produtividade de raízes de mandioca variou de 12,76 a 17,20 t ha-1, porém não foi detectada diferença estatística entre os tratamentos. Foram identificadas 16 espécies de fungos micorrízicos arbusculares e foi constatado que o número de esporos de algumas espécies sofreu alteração da sua freqüência de ocorrência com os cultivos. O pré-cultivo com sorgo aumentou o número de propágulos infectivos em relação ao controle, demonstrando que o uso de espécies de plantas com capacidade para elevar o potencial de inóculo pode ser um bom caminho alternativo para resolver o problema de produção de inóculo para culturas micotróficas em campo.The effect of pre-crops on the inoculum potential (IP of arbuscular mycorrhizal fungi (AMF and yield of cassava was evaluated on a Red-Yellow Podzolic soil in the field. The experiment was carried out at Embrapa-Centro Nacional de Pesquisa de Agrobiologia (CNPAB, Seropédica, RJ, Brazil, in plots with 30 m² arranged in a randomised block design with four replications. As pre-crops, four legume species were used: Canavalia ensiformis, Mucuna aterrina, Crotalaria juncea and Cajanus cajan; and a grass Sorghum bicolor (BR 005 in relation to

  16. Biologic dinitrogen fixation and nutrient cycling in cover crops and their effect on organic Conilon coffeeFixação biológica de nitrogênio e ciclagem de nutrientes por plantas de cobertura e seus efeitos sobre café Conilon orgânico

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    José Antonio Azevedo Espindola

    2011-08-01

    Full Text Available Notwithstanding its relevance, studies regarding nutrient cycling and biological dinitrogen fixation in Conilon coffee (Coffee canephora cv. Conilon associated with cover plants are very scarce. Aiming to evaluate the contribution of cover crops for organic conilon production, a field experiment was carried out consisting of Pennisetum glaucum, and legume species Canavalia ensiformis, Mucuna deeringiana and Cajanus cajan (inoculated and non inoculated cultivated between coffee trees, and spontaneous vegetation as cover crops. The experiment was carried out in Espírito Santo State- Brazil, in a 6.5 years old coffee crop production system. Chemical analyses of soil and vegetative parts of spontaneous and cover crops, as well as coffee leaf nutrients concentration were performed. Biological Nitrogen Fixation (BNF was determined by the natural abundance method. BNF contributed with about 80% of the nitrogen accumulated by the leguminous plants, corresponding to 27 - 35 kg of N ha-1. Concentration and accumulation of nutrients varied among cover crops. Rhizobium inoculation did not influence nutrient cycling or BNF. Legume plants partially supplied the nitrogen requirements of Conilon coffee. No significant effect of the treatments was observed on the nutrient concentration of Conilon coffee or on plant growth.Apesar de sua relevância, estudos sobre a ciclagem de nutrientes e fixação biológica do nitrogênio (FBN em café Conilon (Coffea canephora cv. Conilon, associadas com plantas de cobertura, são escassos. Objetivou-se, com este trabalho avaliar a ciclagem de nutrientes, a FBN e o efeito que plantas de cobertura podem causar em lavoura de C. canephora cv. Conilon, sob manejo orgânico. O experimento foi conduzido no Estado do Espírito Santo - Brasil, em uma lavoura de café sob manejo orgânico, com 6,5 anos. Os tratamentos consistiram de testemunha (ausência de plantas de cobertura, Pennisetum glaucum e as leguminosas Canavalia

  17. Digestão dos carboidratos de alimentos volumosos em eqüinos Digestion of carbohydrates of forages in horses

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    Eliane da Silva Morgado

    2009-01-01

    Full Text Available Neste estudo, foram realizados dois ensaios com os objetivos de avaliar o fracionamento dos carboidratos de alimentos volumosos e estimar a digestibilidade desses nutrientes em dois ensaios de digestão com eqüinos. No ensaio 1, foram utilizados cinco eqüinos em delineamento de blocos casualizados, com sete tratamentos - fenos de alfafa (Medicago sativa; amendoim forrageiro (Arachis pintoi; desmódio (Desmodium ovalifolium; guandu (Cajanus cajan; macrotiloma (Macrotyloma axillare; estilosantes (Stylosanthes guianensis; ou coastcross (Cynodon dactylon - avaliados pela técnica de sacos de náilon móveis. No ensaio 2, foram utilizados quatro eqüinos em delineamento quadrado latino 4 × 4, alimentados com feno de coastcross em quatro tipos de moagem com o objetivo de avaliar se a redução do tamanho de partícula interfere na digestibilidade dos carboidratos. Os resultados comprovaram que os eqüinos possuem alta eficiência na digestão dos carboidratos não-fibrosos e de suas frações hidrolisáveis e rapidamente fermentáveis. Os fenos de amendoim forrageiro, estilosantess e macrotiloma apresentaram elevada digestibilidade dos carboidratos fibrosos e não-fibrosos, enquanto a digestibilidade de todos os nutrientes do amendoim forrageiro foi superior a 70%, o que indica potencial para uso desta leguminosa em dietas para eqüinos. O processamento do feno de coastcross não influenciou a digestibilidade das frações dos carboidratos fibrosos e não-fibrosos. A análise dos carboidratos fibrosos e não-fibrosos é um bom indicativo do valor nutricional dos alimentos e pode ser incluída na avaliação da qualidade de alimentos para eqüinos.This work was carried out to evaluate the fractions of carbohydrates and estimate the apparent digestibility of these nutrients in two digestion assays with horses. In assay 1, five horses were allotted to a complete randomized blocks design and treatments were seven forages hays, alfalfa (Medicago sativa

  18. Restos vegetais e adubação nitrogenada na micorrização e produtividade do arroz de terras altas em sistema plantio direto = Plant residues and nitrogen fertilization on the mycorrhization and upland rice yield on no tillage system

    Directory of Open Access Journals (Sweden)

    Tânia Oshiro Nosse

    2008-10-01

    Full Text Available O objetivo deste trabalho foi verificar a influência de restos vegetais e da adubação nitrogenada em cobertura na micorrização (colonização, número de esporos dos fungos micorrízicos arbusculares e produtividade de grãos de arroz de terras altas em plantio direto. Oexperimento foi conduzido na Fazenda Experimental da Unesp/Campus de Ilha Solteira, município de Selvíria (Estado do Mato Grosso do Sul. Nas parcelas principais foram testadas as leguminosas: crotalária (Crotalaria juncea L., guandu (Cajanus cajan L. e a mucuna-preta(Stizolobium aterrimum Piper & Tracy e as gramíneas: milho (Zea mays L., milheto (Pennisetum americanum L. e sorgo-granífero (Sorghum bicolor L., mais o pousio (vegetação espontânea, como cobertura de solo. Nas subparcelas, após a semeadura do arroz, foi utilizada adubação nitrogenada em cobertura (zero e 75 kg ha-1 de N, na forma de uréia. A produtividade de grãos do arroz foi influenciada pelos restos vegetais, mas não pelas doses ou pela interação. O sorgo mostrou influência negativa sobre a produtividade do arroz. A adubação nitrogenada influenciou a esporulação, mas não a colonização micorrízica ou a produtividade de grãos, na época da colheita.Não houve correlação entre as variáveis estudadas.The objective of this research was to verify the influence of plant residues and the nitrogen fertilization in covering on the mycorrhization (colonization and number of spores of the arbuscular mycorrhizal fungi, and grain yield of upland rice grown under a no tillage system. The experiment was conducted at the Experimental Farm Station of Unesp/Ilha Solteira Campus, at Selvíria (Mato Grosso do SulState. In the main plots, the leguminous were tested: sun hemp (Crotalaria juncea L., pigeon pea (Cajanus cajan L. and velvet bean (Stizolobium aterrimum Piper & Tracy and the grass: corn (Zea mays L., millet (Pennisetum americanum L. and grain sorghum (Sorghumbicolor L., plus no planted area

  19. Características químicas de solo e rendimento de fitomassa de adubos verdes e de grãos de milho, decorrente do cultivo consorciado Soil chemical characteristics and green manure yield in a corn intercropped system

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    Reges Heinrichs

    2005-02-01

    Full Text Available A adubação verde é uma das formas de aporte de matéria orgânica ao solo. O sistema de cultivo consorciado de culturas pode ser uma alternativa para aumentar a reciclagem de nutrientes e melhorar a produtividade. Para avaliar o sistema consorciado de adubos verdes com o milho, foram estudadas as características químicas do solo, a produção de matéria seca, a composição mineral de adubos verdes e o rendimento de grãos de milho, num experimento realizado em campo, entre 1995 e 1997, em solo classificado como Nitossolo Vermelho eutrófico. O milho foi semeado no espaçamento de 90 cm nas entrelinhas, perfazendo, aproximadamente, 50.000 plantas por hectare. Os tratamentos constaram de quatro espécies de adubos verdes: mucuna anã [Mucuna deeringiana (Bort. Merr], guandu anão (Cajanus cajan L., crotalária (Crotalaria spectabilis Roth e feijão-de-porco (Canavalia ensiformis L. e um tratamento-testemunha, sem cultivo consorciado. Essas espécies foram semeadas sem adubação, no meio da entrelinha, em duas épocas: simultânea ao milho e 30 dias após. O delineamento experimental foi o de blocos ao acaso em parcelas subdivididas, com quatro repetições. O feijão-de-porco apresentou maior produção de fitomassa e acúmulo de N, P, K, Ca, Mg e S. No primeiro ano de cultivo, o rendimento de grãos de milho não foi influenciado pelo cultivo consorciado com adubos verdes; no entanto, no segundo, a produção foi beneficiada pelo consórcio com feijão-de-porco.Green manure is one way of supplying organic matter to soil. The mixed cultivation of crops may be an alternative to increase nutrient cycling and to improve productivity. To evaluate intercrops of green manure and corn, soil chemical characteristics, green manure dry matter production and its mineral composition and corn yield were determined in a field experiment carried out between 1995 and 1997 on an Aleudalf Soil in Piracicaba, state of São Paulo, Brazil. Corn was sown in

  20. Sistemas de cultivo, sucessões de culturas, densidade do solo e sobrevivência de patógenos de solo Cropping systems and previous crops on soil density and survival of soil-borne pathogens

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    Eliane Divina de Toledo-Souza

    2008-08-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito de sistemas de manejo do solo e de cultivos prévios ao plantio do feijoeiro (Phaseolus vulgaris L. sobre a densidade do solo e as populações de Rhizoctonia spp. e de Fusarium spp. Os cultivos prévios incluíram as leguminosas: guandu-anão (Cajanus cajan, estilosantes (Stylosanthes guianensis cv. Mineirão e crotalária (Crotalaria spectabilis; e as gramíneas: milheto (Pennisetum glaucum cv. BN-2, sorgo granífero (Sorghum bicolor cv. BR 304, capim-mombaça (Panicum maximum cv. Mombaça, braquiária (Brachiaria brizantha cv. Marandu e milho (Zea mays consorciado com braquiária. As culturas utilizadas no cultivo prévio foram semeadas nos verões de 2002, 2003 e 2004, e os plantios de feijoeiro, cultivar BRS Valente, foram realizados nos invernos subseqüentes de 2003, 2004 e 2005, com irrigação por pivô central. Os restos culturais dos cultivos eram incorporados ao solo, no plantio convencional, e ficavam à superfície, no plantio direto. De modo geral, as maiores populações de Fusarium spp. e Rhizoctonia spp. e as maiores densidades de solo foram encontradas no solo cultivado em plantio direto. As maiores populações de Rhizoctonia spp. foram observadas em solos mais adensados. As leguminosas geralmente aumentaram populações desses patógenos e devem ser evitadas como culturas prévias ao cultivo do feijoeiro, em ambos os sistemas de cultivo. Plantios prévios de gramíneas, em geral, são supressores das populações de Rhizoctonia spp. e de Fusarium spp. no solo.The objective of this work was to evaluate the effect of cropping systems and crops cultivated previously to common beans (Phaseolus vulgaris L. on soil density and soil populations of Rhizoctonia spp. and Fusarium spp. Previous crops included the following legumes: Cajanus cajan,Stylosanthes guianensis cv. Mineirão and Crotalaria spectabilis; and the following grasses: Pennisetum glaucum (cv. BN-2, millet, Sorghum bicolor

  1. COVER CROPS EFFECTS ON THE DEVELOPMENT OF UPLAND RICE UNDER ORGANIC FARMING SYSTEM PLANTAS DE COBERTURA DE SOLO E SEUS EFEITOS NO DESENVOLVIMENTO DA CULTURA DO ARROZ DE TERRAS ALTAS EM CULTIVO ORGÂNICO

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    Roberta Paula de Jesus

    2007-12-01

    Full Text Available

    This research was conducted in an experimental area in Santo Antônio de Goiás (16º28'S, 49º17'W and altitude 823 m, Brazil, during the months of June, 2004, and March, 2005. The upland rice variety Aimoré was used along with several cover crops aiming to evaluate leaf area, number of tillers, dry matter, and nitrogen content in the phytomass during the rice crop cycle. The experimental design was the randomized blocks one, with five treatments and four replications. The treatments consisted of different cover crops, such as velvet bean (Mucuna aterrima, sunn hemp (Crotalaria juncea, dwarf pigeon pea (Cajanus cajan, broom sorghum (Sorghun bicolor, and a check treatment with spontaneous vegetation growing among the rice plants. The leguminous plants, specially C. juncea, presented better results in tillering production, leaf area index, dry matter yield, and accumulated nitrogen content, if compared to the treatments where grasses were used as soil cover crop. It was concluded that rice presented a satisfactory development in the different soil cover treatments, specially after leguminous cultivation.

    KEY-WORDS: Rice, green manure, leaf area index, dry matter.

    O experimento foi conduzido numa área experimental em Santo Antônio de Goiás (16º28'S, 49º17'W e altitude de 823 m, no período de junho de 2004 a março de 2005. Utilizou-se a cultivar Aimoré de arroz de terras altas, em seqüência a diferentes plantas de cobertura de solo, com o objetivo de avaliar o índice de área foliar (IAF, número de afilhos, acúmulo de massa de matéria seca (MMS e o teor de nitrogênio acumulado na fitomassa durante o ciclo da cultura do arroz. O delineamento experimental foi o de blocos ao acaso, com cinco

  2. Crescimento e produtividade do sorgo forrageiro BR 601 sob adubação verde Growth and yield of forage sorghum cv. BR 601 under green manure

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    Romeu C. Andrade Neto

    2010-02-01

    Full Text Available O efeito da adubação verde sobre o crescimento e a produtividade do sorgo forrageiro BR 601, foi estudado em experimento desenvolvido na UFERSA, em Mossoró, RN, no qual o delineamento utilizado foi em blocos casualizados completos, em esquema de parcelas subdivididas no tempo com três repetições. Testaram-se sete espécies de leguminosas, Mucuna aterrima, Canavalia ensiformis, Cajanus cajan, Dolichos lab-lab, Crotalaria juncea, Crotalaria spectabilis e Vigna unguiculata, uma mistura das leguminosas mais milho, sorgo e girassol, e a vegetação espontânea, como testemunha. Determinaram-se, para cada tratamento, a quantidade de massa verde, massa seca e teores de N, P, K, Na, Ca e Mg da parte aérea, a partir dos quais foi calculada a quantidade de nutrientes acumulada por hectare. As quantidades de matéria fresca e seca da parte aérea, altura de plantas e número de folhas do sorgo semeado após a incorporação dos adubos verdes, foram avaliadas aos 20, 40, 60, 80 e 100 dias após o plantio. A Mucuna aterrima mostrou-se a melhor opção como adubo verde em virtude de proporcionar os maiores valores das características avaliadas ao final do ciclo do sorgo podendo-se, também, recomendar a Crotalaria juncea e o Dolichos lab-lab, o qual apresentou a maior massa seca e quantidade de nutrientes na parte aérea.The effect of green manure on growth and yield of forage sorghum BR 601 was studied in a trial carried out at the UFERSA, Mossoró, RN, Brazil. The experimental design was completely randomized blocks in a scheme of split plots in time, with three replications. Treatments tested were seven legume species, Mucuna aterrima, Canavalia ensiformis, Cajanus cajan, Dolichos lab-lab, Crotalaria juncea, Crotalaria spectabilis, and Vigna unguiculata, a mixture of the legumes plus corn, sorghum and sunflower, and spontaneous vegetation as a control. For each treatment the amount of fresh mass, dry mass and contents of N, P, K, Na, Ca and Mg in

  3. Coberturas vegetais, doses de nitrogênio e inoculação de sementes com Azospirillum brasilense em arroz de terras altas no sistema plantio direto Cover crops, nitrogen rates and seeds inoculation with Azospirillum brasilense in upland rice under no-tillage

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    Douglas de Castilho Gitti

    2012-01-01

    Full Text Available O arroz é importante fonte de energia e proteínas para a população mundial, principalmente na Ásia e Oceania. No Brasil, juntamente com o feijão, constitui a base da alimentação. Tecnologias sustentáveis que reduzam custos da produção e aumentem a produtividade do arroz podem garantir seu suprimento em períodos de alta demanda. O objetivo deste trabalho foi avaliar diferentes coberturas vegetais antecessoras (milheto [Pennisetum americanum], crotalária [Crotalaria juncea], guandu [Cajanus cajan], braquiária [Brachiaria ruziziensis], milheto + crotalária e milheto + guandu, doses de nitrogênio (N em cobertura (0, 40, 80 e 120 kg ha-1 e o efeito da inoculação de sementes com Azospirillum brasilense no arroz de terras altas em sistema plantio direto no desenvolvimento e na produtividade. Utilizou-se o delineamento experimental em blocos casualizados em esquema fatorial 6x4x2 com quatro repetições. O estudo foi desenvolvido em um Latossolo Vermelho, em Selvíria, Estado do Mato Grosso do Sul, Brasil, em 2011/2012. O cultivo do guandu antecedendo o arroz proporcionou maior produtividade do arroz somente em comparação a B. ruziziensi. A produtividade do arroz em função das doses de N em cobertura se ajustou a uma função quadrática. Não houve influência da inoculação de sementes com A. brasilense sobre a produtividade do arroz, porém houve interação entre a inoculação e as coberturas vegetais sobre o teor de N foliar, número de panículas por m², matéria seca de plantas de arroz e a massa de cem grãos.Rice is an important source of energy and protein for the world population, mainly in Asia and Oceania. In Brazil, together with common beans, it is the population's basic food. Sustainable technologies that reduce rice production costs and increase productivity can warrant its supply on period of high demand. The objective of this study was to evaluate different preceding cover crops (millet [Pennisetum americanum

  4. Calidad de las semillas de accesiones de leguminosas colectadas en la región oriental de Cuba (Nota técnica Seed quality of legume accessions collected in the eastern region of Cuba (Technical note

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    Yolanda González

    2011-03-01

    Full Text Available Se efectuó una misión de colecta durante el 2006 en la región oriental de Cuba, que comprendió zonas de las provincias de Granma, Guantánamo, Holguín, Bayamo, Las Tunas y Camagüey. Se colectaron semillas de 95 accesiones de leguminosas, de ellas 68 herbáceas y 27 arbóreas. Posteriormente se hizo una prueba de germinación, para conocer su calidad (con corte de cubierta. Para ello se utilizaron placas de Petri sobre arena de río. El número de semillas utilizadas fue variable en cada accesión (según la cantidad colectada y solo se empleó una réplica (sin diseño estadístico. Los conteos de germinación se realizaron según las normas internacionales. Las semillas de mejor calidad correspondieron a las especies arbóreas, aunque presentaron baja germinación las de Cajanus cajan, Cassia siamea, Peltophorum ferrugianum y Leucaena leucocephala, procedentes de la zona de Floro-Pérez en la provincia de Holguín. Todas mostraron 100% de sobrevivencia. La calidad de las semillas dependió del tiempo que estuvieron en el campo después de la etapa de maduración de las legumbres. A través de esta misión de colecta se incrementó el germoplasma de la EEPF «Indio Hatuey» con 64 nuevas accesiones. Se recomienda la continuidad de la colecta de germoplasma en otras zonas del país, con énfasis en las plantas arbóreas, lo que permitirá lograr una mayor diversidad a partir de su uso en los sistemas de producción agropecuaria, así como llevar a cabo las pruebas de calidad correspondientes.A collection mission was conducted during 2006 in the eastern region of Cuba, which comprised zones of the provinces Granma, Guantánamo, Holguín, Bayamo, Las Tunas and Camagüey. Seeds from 95 legume accessions, 68 herbaceous ones and 27 trees, were collected. Afterwards, a germination test was conducted, to know their quality (with seed coat cut. For that purpose, Petri dishes were used on river sand. The number of seeds used was variable in each

  5. Biomassa microbiana, colóides orgânicos e nitrogênio inorgânico durante a vermicompostagem de diferentes substratos Microbial biomass, organic colloids and inorganic nitrogen, during vermicomposting of some substrates

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    Adriana Maria de Aquino

    2005-11-01

    Full Text Available O objetivo deste trabalho foi avaliar as modificações que ocorrem no substrato durante a vermicompostagem, utilizando-se biomassa microbiana, formas de N mineral, relação C/N e colóides orgânicos totais. As seguintes misturas foram utilizadas como substratos para a vermicompostagem: esterco, esterco + bagaço na proporção de 1:1, e esterco + bagaço + guandu na proporção de 2:1:1. As misturas foram feitas com base em volume e acondicionadas em canteiros de 0,4 m³. Trezentas minhocas da espécie Eisenia foetida e 100 da Eudrilus eugeniae foram introduzidas em cada canteiro. A cada 20 dias, aproximadamente, num total de 126 dias, foram realizadas as amostragens para a realização das análises. O delineamento experimental adotado foi o de blocos ao acaso, com três repetições em esquema fatorial de 3x8 (três substratos e oito épocas de amostragens. As modificações na biomassa microbiana, quociente metabólico e o conteúdo de nitrato e amônio, não possibilitaram caracterizar a época da maturidade dos vermicompostos. O esterco leva cerca de 70 dias para apresentar relação C/N estável, o esterco + bagaço e o esterco + bagaço + guandu levam 90 dias. A determinação dos colóides orgânicos totais é bastante simples, mas não possibilita caracterizar a maturidade dos vermicompostos.The objetive of this work was to evaluate modifications in the microbial biomass, metabolic quotient, types of mineral nitrogen, C/N ratio, and total organic colloids concentration. The following variations in the substrate composition were utilized: pure cattle manure, pure cattle manure + sugarcane bagasse 1:1 ratio, and pure cattle manure + sugarcane bagasse + leaves of Cajanus cajan 2:1:1 ratio. The mixture was made on volume bases and the substrate composition filled boxes of 0.4 m³. Three hundred earthworms of the species Eisenia foetida and 100 of Eudrillus eugeniae were introduced in the boxes. Every 20 days, approximately, samples were

  6. Fluxo difusivo de micronutrientes catiônicos afetado pelo tipo, dose e época de incorporação de adubos verdes ao solo Diffusive flux of cationic micronutrients as affected by type, dose and timing of green manure incorporation into soil

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    Rodinei Facco Pegoraro

    2006-12-01

    Full Text Available Os solos brasileiros, principalmente os do cerrado, são bastante intemperizados e pobres em alguns micronutrientes catiônicos na solução do solo. A utilização de técnicas de manejo, como a adubação verde, pode favorecer o fluxo difusivo (FD e a disponibilidade desses nutrientes para as plantas. O presente trabalho visou avaliar se a incorporação de adubos verdes ao solo, em diferentes doses e épocas, modifica o FD e a forma iônica de transporte dos micronutrientes Zn, Cu, Fe e Mn no solo. Para tanto, foram incorporados dois resíduos vegetais largamente cultivados como adubo verde: o feijão guandu (Cajanus cajan ou o milheto (Pennisetum americanum por diferentes períodos (0, 15, 25, 35, 45 e 55 dias e doses (0, 9, 18 e 36 t ha-1 num Latossolo Vermelho, argiloso, em condições de laboratório. Para avaliar o FD, utilizaram-se resinas de troca aniônica (positivamente carregada e de troca catiônica (negativamente carregada na forma de lâmina, incubadas junto ao solo em câmaras de difusão durante 15 dias. Os resultados obtidos demonstraram que houve aumento do FD do Cu e do Fe com o aumento das doses de material vegetal, principalmente no início do período de incubação, e maior fluxo desses dois micronutrientes para a resina aniônica em relação à catiônica, possivelmente por ser o seu transporte no solo mais dependente da formação de complexos organometálicos com carga líquida negativa. Já para Zn e Mn, o fluxo difusivo foi maior para a resina catiônica. O aumento do tempo de incubação favoreceu o fluxo difusivo de Mn e Zn e reduziu o do Cu e Fe.Brazilian soils, especially in the Cerrado region, are highly weathered and poor in some cationic micronutrients in the soil solution. Diffusion is the main transport mechanism to the root surface. The adoption of management practices such as the use of green manures might favor the diffusive flux (DF and availability of micronutrients to plants. The present experiment

  7. Phytosociological aspects and weed management using cover crops on organic conilon coffee plantations / Aspectos fitossociológicos e manejo de plantas espontâneas utilizando espécies de cobertura em cafeeiro Conilon orgânico

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    José Antonio Azevedo Espindola

    2010-09-01

    Full Text Available Weeds can bring benefits to agriculture, but when incorrectly managed they can compete with commercial crops for resources. The objective of this work was to evaluate the effect that cover crops, associated with Coffea canephora cv. Conilon, imposes to the weed community. Cover crops were planted between the rows of a 6.5 years old organic coffee plantation spaced 2.0 x 1.5 m. The experiment was arranged in a complete randomized block design, with four replications, in a factorial scheme with the following treatments: control, Pennisetum glaucum and the legume plants: Canavalia ensiformis, Mucuna deeringiana and Cajanus cajan, with and without Rizobium inoculation. Cover crops dry weight and nutrient contents on coffee trees were determined. Weeds density, frequency, relative abundance, importance value index and plant similarity index were also determined. Twenty seven weed species were identified, with special emphasis on Bidens subalternans and Commelina benghalensis. Cover crops promote modifications on the succession dynamics of weeds and do not interfere with the development of the coffee trees. Canavalia ensiformis, Mucuna deeringiana and Pennisetum glaucum help on weed control.As plantas espontâneas competem com as culturas por recursos escassos, quando mal manejadas. No entanto, podem trazer benefícios à agricultura. O objetivo desse trabalho foi avaliar o efeito que plantas de cobertura, consorciadas com Coffea canephora cv. Conilon, impõem à comunidade de plantas espontâneas. Plantas de cobertura foram semeadas nas entrelinhas de um cafezal de 6,5 anos conduzido sob manejo orgânico, com espaçamento de 2,0 x 1,5 m. O delineamento experimental foi de blocos ao acaso, com quatro repetições, num arranjo fatorial com tratamentos adicionais: testemunha, milheto – Pennisetum glaucum, e as leguminosas feijão-de-porco – Canavalia ensiformis, mucuna-anã – Mucuna deeringiana, e feijão-guandu – Cajanus cajan, com e sem

  8. Níveis de farelo de melancia forrageira em dietas para ovinos Levels of forage watermelon meal in diets for sheep

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    Raimundo Luiz Nunes Vaz da Silva

    2009-06-01

    Full Text Available Com o objetivo de estudar a inclusão de 20, 35, 50, 65 ou 80% de farelo de melancia forrageira (Citrullus lanatus cv. citroides (FMF em dietas à base de feno de guandu (Cajanus cajan, foi realizado um ensaio de digestibilidade utilizando-se 20 cordeiros com 6 meses de idade e peso vivo de 11,2 ± 1,2 kg. O experimento constou de 20 dias de adaptação e 5 de coleta total de sobras e de fezes. O farelo de melancia forrageira e o feno de guandu apresentaram 95,3 e 95,75% de matéria seca (MS, 18,7 e 16,8% de proteína bruta (PB e 38,8 e 64,3% de fibra em detergente neutro (FDN. Os níveis de farelo de melancia forrageira determinaram aumento quadrático nos consumos de MS, MO, PB, FDN, FDA, hemicelulose e carboidratos totais. O consumo de extrato etéreo não foi alterado, mas o consumo de CNF teve aumento linear conforme aumentaram os níveis de farelo de melancia forrageira. Os coeficientes de digestibilidade de MS (58%, PB (69%, FDN (41% e EE (85% não foram influenciados pelo nível de farelo de melancia forrageira. As digestibilidades de MO (54 a 62%, FDA (36 a 49% e carboidratos totais (47 a 58% variaram linear e positivamente, enquanto a digestibilidade da hemicelulose teve comportamento inverso. As dietas proporcionaram consumo de matéria seca que atendeu a exigência dos animais. Os coeficientes de digestibilidade observados foram satisfatórios. O farelo de melancia forrageira pode ser indicado para compor dietas à base de feno de guandu em níveis de 35 a 66% da matéria seca.In order to study the inclusion of forage watermelon (Citrullus lanatus var. Citroides meal (FWM in pigeon pea hay based diets (Cajanus cajan (PPH, a digestibility trial in sheep was carried out for testing five levels of FWM (20, 35, 50, 65 and 80%. Twenty lambs with an average age of six months and live weight of 11.2 kg (± 1.2 were used. The experimental period was twenty days for adaptation and five days for total refuses and feces collection. The chemical

  9. Produtividade do cafeeiro Mundo Novo enxertado e submetido à adubação verde antes e após recepa da lavoura Productivity of grafted coffee during intercropping with five leguminous species in the western region of São Paulo State, Brazil

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    Edison Martins Paulo

    2006-01-01

    Full Text Available Estudaram-se a produção e o crescimento do cafeeiro Mundo Novo (Coffea arabica L. enxertado sobre o Apoatã IAC 2258 (Coffea canephora Pierre ex Froehner submetido à adubação verde com as seguintes espécies leguminosas: crotalária espectábilis (Crotalaria spectabilis Roth., crotalária júncea (Crotalaria juncea L., guandu [Cajanus cajan (L. Millsp.], mucuna anã (Stizolobium deeringeanum Bort. e soja IAC 9 [Glycine max (L. Merril] e um tratamento testemunha sem plantas leguminosas. As leguminosas foram semeadas a 50 cm da projeção da copa dos cafeeiros e incorporadas no florescimento. O experimento foi desenvolvido no Pólo Regional de Desenvolvimento Tecnológico dos Agronegócios da Alta Paulista, em Adamantina, no período de 1989 a 1995. Adotou-se o delineamento estatístico de blocos ao acaso com seis tratamentos e cinco repetições. Os adubos verdes crotalária espectábilis, crotalária júncea, mucuna anã e soja, durante o período experimental, e a crotalária espectábilis após a recepa, não diminuíram a produção do cafeeiro. O guandu, embora tenha aumentado o teor de matéria orgânica do solo, foi a única leguminosa que diminuiu a produção e o diâmetro do caule dos cafeeiros. O guandu e a crotalária júncea, respectivamente, produziram as maiores quantidades de fitomassa seca. A produção do café se correlacionou inversamente com a fitomassa seca das leguminosas e positivamente com altura e diâmetro do caule do cafeeiro.Yield of arabica coffee (Coffea arabica of grafted onto robusta coffee (Coffea canephora Pierre ex Froehner Apoatã IAC 2258 was evaluated during six years of intercropping with five leguminous species: sunn hemp (Crotalaria juncea L., Crotalaria spectabilis Roth., dwarf velvet bean (Stizolobium deeringeanum Bort., soybean Glycine max (L. Merryl] and pigeon pea [Cajanus cajan (L. Millsp.] in the Western region of São Paulo State, Brazil, from 1989 to 1995. Leguminous species were sown 50 cm

  10. Digestibilidade dos nutrientes de alimentos volumosos determinada pela técnica dos sacos móveis em eqüinos Nutrient digestibility of forage feed determined using mobile bag technique in horses

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    Vinícius Pimentel Silva

    2009-01-01

    Full Text Available Objetivou-se estimar a digestibilidade de nutrientes de forrageiras em eqüinos utilizando-se a técnica de sacos de náilon móveis. Foram avaliados alfafa (Medicago sativa, amendoim forrageiro (Arachis pintoi, desmódio (Desmodium ovalifolium, estilosantes (Stylosanthes guianensis, guandu (Cajanus cajan, macrotiloma (Macrotyloma axillare e capim-coastcross (Cynodon dactylon cv. coastcross. O delineamento foi em blocos inteiramente casualizados com sete alimentos e cinco blocos (animais. Foram utilizados cinco eqüinos mestiços com 17 a 27 anos de idade e peso vivo médio de 350 kg. O ensaio teve duração de 12 dias: três para a adaptação às baias, cinco para inserção gástrica dos sacos através de sonda nasogástrica e quatro de coleta dos sacos nas fezes. No período pré-experimental de 30 dias, os animais foram mantidos em piquetes com dieta composta de 80% de feno de coastcross e 20% de concentrado. Na confecção dos sacos, utilizou-se náilon com porosidade de 45 µ e dimensão de 7,5 × 2 cm. Em cada saco, foram inseridos 510 mg de matéria seca de amostra do alimento. Os coeficientes de digestibilidade dos nutrientes das forragens foram calculados considerando o resíduo obtido no saco. A digestibilidade dos nutrientes do amendoim, estilosantes e macrotiloma foram superiores à da demais forrageiras, com destaque para a digestibilidade da proteína bruta, cujos valores foram de 91,4; 94,9 e 97,0%, respectivamente. O amendoim e macrotiloma apresentaram digestibilidade da fibra em detergente neutro de 72,3 e 65,2% e da fibra em detergente ácido de 70,9 e 59,4%, respectivamente. O amendoim forrageiro, macrotiloma e estilosantes apresentam digestibilidade dos nutrientes satisfatória e têm potencial para o uso em dietas para eqüinos.This work was carried out to evaluate the nutrient digestibility of forages using mobile bags technique in horses. The forages were lucerne (Medicago sativa, peanut (Arachis pintoi, desmodio

  11. Morphology of olfactory sensilla and its role in host plant recognition by Neomegalotomus parvus (Westwood (Heteroptera: Alydidae

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    Maurício Ursi Ventura

    2005-07-01

    Full Text Available The external morphology of the sensilla, including pores and permeable points on the cuticle of the antennae of adults of Neomegalotomus parvus (West. (Heteroptera: Alydidae was investigated with regard to their olfactory function. Behavioural evidence of olfaction, the importance of the different antennal segments in locating the host under still-air, and the responses of the insects to plants' scents and to pheromones in wind assays were also studied. With the scanning electronic microscope mechanoreceptor sensilla were found on the four segments of the antennae of N. parvus. The density of the sensilla was greater on the 4th segment. On the apical segment, sensilla with olfactory morphological traits were observed. Bugs with intact antennae located food more frequently and faster than those with blocked or amputated antennae. N. parvus preferred intact seeds to sealed seeds, but there was no preference when the last segment of the antenna was blocked. In wind tunnel, bugs were attracted to mature and green pods, and to hexanic extract of mature pods of pigeon pea, Cajanus cajan (L. Millsp.; there was no response to panicles of rice, Oryza sativa L. or to the male pheromone.A morfologia externa dos sensilos de adultos de Neomegalotomus parvus (Westw. (Het.: Alydidae, incluindo poros e pontos permeáveis na cutícula, foi estudada em microscópio eletrônico de varredura, em relação à função olfativa. A evidência comportamental do olfato e a importância dos diferentes segmentos antenais na localização do hospedeiro, em condições de ausência de movimentação do ar, foram também estudados. Sensilos mecanoreceptores foram observados nos quatro segmentos da antena de N. parvus, sendo no entanto, a densidade maior no quarto segmento. Nos segmentos apicais, foram observados sensilos com características morfológicas olfativas. Percevejos com antenas intactas localizaram o alimento com mais freqü��ncia e rapidez do que aqueles com

  12. Fitorremediação de solos contaminados com tebuthiuron utilizando-se espécies cultivadas para adubação verde Phytoremediation of tebuthiuron-contaminated soils using species cultivated for green manure

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    F.R. Pires

    2005-12-01

    Full Text Available O emprego da fitorremediação na despoluição de solos contaminados com compostos orgânicos, inclusive herbicidas, vem sendo pesquisado ultimamente. Como o tebuthiuron pode causar sério impacto ambiental, por ser muito utilizado, apresentar longo efeito residual no solo e possibilidade de contaminação do lençol de água subterrâneo, desenvolveu-se este trabalho com o objetivo de avaliar a capacidade de sete espécies vegetais na despoluição de solos contaminados com esse herbicida. As espécies avaliadas neste experimento foram: Cajanus cajan, Canavalia ensiformes, Dolichos lablab, Pannisetum glaucum, Estizolobium deeringianum, Estizolobium aterrimum e Lupinus albus. Elas foram semeadas e cultivadas, por 60 dias, em vasos cujo solo recebeu quatro doses do tebuthiuron (0,0; 0,5; 1,0; e 1,5 kg ha-1. As testemunhas foram constituídas por vasos sem planta, aos quais foram aplicadas as mesmas doses de herbicidas. Aos 60 dias após a semeadura, colheu-se a parte aérea de todas as plantas, sendo semeada, nos mesmos vasos, Avena strigosa, utilizada como planta indicadora, para realização do bioensaio. Depois de 60 dias da semeadura da espécie bioindicadora, esta foi colhida, sendo avaliadas as seguintes características: altura de plantas, sintomas de toxicidade e biomassa seca da parte aérea das plantas. Até a dose de 0,5 kg ha-1 de tebuthiuron, a espécie que melhor fitorremediou esse herbicida no solo foi L. albus. Quando o solo foi tratado com 1,0 kg ha-1 de tebuthiuron, C. ensiformes foi a espécie que melhor fitorremediou o herbicida. Isso foi concluído com base na maior altura de plantas, biomassa seca da parte aérea e menor toxicidade de A. strigosa, quando foi cultivada em sucessão a essas plantas remediadoras. Nenhuma das espécies avaliadas cresceu em solo que recebeu a maior dose de tebuthiuron (1,5 kg ha-1.Phytoremediation of soil contaminated by organic compounds, including herbicides, is being widely investigated. The

  13. Atributos físicos de um Latossolo Vermelho e produtividade de culturas cultivadas em sucessão e rotação Physical properties of an Oxisol and crops yield under succession and rotation

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    Silvio Aymone Genro Junior

    2009-02-01

    to 2001: 1 succession wheat / soybean, 2 rotation corn / oat / corn+ Cajanus cajan L. Millsp / wheat / soybean / wheat, 3 rotation Cajanus cajan L. Millsp / wheat / soybean / wheat / soybean / oat, and 4 rotation Crotalaria juncea L. / wheat / soybean / oat / corn / wheat. In October of 2000 and 2001, soil samples were collected to determine physical properties. In all years, the crops yield was measured. The Oxisol cultivated under no-tillage had a high compaction degree, with soil bulk density greater and macroporosity smaller than the critical limit for clay texture. The largest limitations were observed below the layer of 0 to 0,03m, because in the uppermost layer soil mobilization by successive operations of fertilization and sowing increases soil porosity and reduces soil density. Thus, greater volume of pores is observed between soil saturation and field capacity, responsible for soil aeration, and between field capacity and wilting point, responsible for storage of plant available water. The use of cover crops once every three years was not efficient to reduce soil compaction. The yield of the subsequent crops was influenced positively in the rotation with Crotalaria juncea, while the other cropping systems did not differ from crop succession.

  14. Avaliação de espécies leguminosas na formação de cafezais no segmento da agricultura familiar no Acre Evaluation of legume species for coffee plants formation in the segment of family farms in Acre

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    Celso Luis Bergo

    2006-03-01

    Full Text Available Leguminosas quando consorciadas com o café e usadas como adubação verde podem contribuir fornecendo nitrogênio e proteção ao solo pela adição de matéria orgânica. O trabalho foi conduzido com o objetivo de avaliar o efeito do uso de leguminosas no sistema de produção de café, no segmento de agricultura familiar, visando promover a implantação e a manutenção dos cafezais de forma técnica e economicamente sustentável. O experimento foi conduzido no período de novembro de 2000 a abril de 2003, em delineamento experimental de blocos ao acaso em esquema de parcelas subdivididas, com quatro repetições. Os tratamentos utilizados nas parcelas foram as leguminosas (Stizolobium atterrimum, Cajanus cajan, Canavalia endiformis e Flemingia congesta e a testemunha (sem leguminosa e, nas subparcelas, duas doses de N (0 e 22 g de N por cova. A Flemingia congesta e a Mucuna aterrima foram as leguminosas que mais influenciaram positivamente a produtividade dos cafeeiros, independente da adubação nitrogenada. Em relação à testemunha, o aumento em produção foi de 109% quando utilizou-se a Flemingia congesta e 52% com a Mucuna aterrima. A Flemingia congesta foi também a leguminosa que melhor controlou as invasoras, dado o volume de fitomassa produzida e a possibilidade de 2 cortes durante um período de doze meses, evidenciando o potencial desta leguminosa na formação de novos cafezais no Acre. Por outro lado, a Canavalia ensiformis, leguminosa que é normalmente utilizada nas entrelinhas dos cafeeiros pelos cafeicultores, do Acre e de outras regiões produtoras, neste trabalho influenciou negativamente a altura das plantas, diâmetro da copa e crescimento dos cafeeiros.Legumes, when associated with coffee crop and used as green manure can contribute with nitrogen and protect the soil by addition of organic matter. The effect of the use legume species in the coffee production system of family farms, was evaluated in order to promote

  15. Comportamento de dois genótipos de milho cultivados em sistema de aléias preestabelecido com diferentes leguminosas arbóreas Behaviour of two maize genotypes grown in alley cropping system pre-established with diferents leguminous trees

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    Andréia Araújo Lima Leite

    2008-12-01

    Full Text Available O cultivo em aléias tem sido recomendado como alternativa para a substituição da agricultura de corte e queima, no trópico úmido, devido à grande capacidade de produção de matéria orgânica e de reciclagem de nutrientes, mas algumas dúvidas quanto à sustentabilidade e à competição interespecífica são persistentes. O objetivo no trabalho foi avaliar a viabilidade da cultura do milho em um sistema de cultivo em aléias de leguminosas arbóreas. O delineamento experimental utilizado foi em blocos casualisados, com quatro repetições dos tratamentos: aléias de sombreiro (Clitoria fairchildiana, ingá (Inga edulis, guandu (Cajanus cajan e leucena (Leucaena leucocephala e uma testemunha sem aléias. Foram avaliadas a remobilização de carbono e nitrogênio, massa de grãos, massa de mil grãos e competição interespecífica entre as cultivares de milho e as leguminosas. A produção de grãos foi maior nas parcelas com C. fairchildiana e L. leucocephala. A produtividade do híbrido de milho foi superior à da variedade em todos os tratamentos. A produtividade e a massa de mil grãos de milho não são negativamente afetadas pela distância da linha da leguminosa arbórea. Esse estudo conclui que o sistema de aléias com leguminosas arbóreas é uma alternativa importante ao manejo sustentável dos agroecossistemas no tropico úmido. Além disso, nessa região a produtividade em grãos na cultura do milho é favorecida no sistema de aléias preeestabelecidas com as leguminosas arbóreas sombreiro, ingá e leucena e pela utilização de genótipos eficientes no aproveitamento do nitrogênio, cujo sincronismo entre a liberação e a absorção do N aplicado por meio das leguminosas deve ser aprimorado.Alley cropping has been recommended as alternative land use to slash-and-burn agriculture in humid tropics. However, interespecific competition between cash crop and hedgerow can reduce this potential. This study aimed to evaluate the

  16. Microclimatic characterization and productivity of coffee plants grown under shade of pigeon pea in Southern Brazil Caracterização microclimática e produtividade de cafeeiros sombreados com guandu no Sul do Brasil

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    Heverly Morais

    2006-05-01

    Full Text Available Recent studies on coffee (Coffea arabica L. cultivation in agroforestry systems in Southern Brazil have shown the potential of partial shading to improve management of this crop. The objective of this work was to evaluate microclimatic conditions and their effects on coffee production of plants shaded with pigeon pea (Cajanus cajan in comparison to unshaded ones, from May 2001 to August 2002 in Londrina, State of Paraná, Brazil. The appraised microclimatic characteristics were: global radiation, photosynthetic and radiation balance; air, leaf and soil temperatures; and soil humidity. Shading caused significant reduction in incident global solar radiation, photosynthetically active radiation and net radiation, and attenuated maximum leaf, air and soil temperatures, during the day. Shade also reduced the rate of cooling of night air and leaf temperatures, especially during nights with radiative frost. Soil moisture at 0-10 cm depth was higher under shade. The shaded coffee plants produced larger cherries due to slower maturation, resulting in larger bean size. Nevertheless, plants under shade emitted less plagiotropic branches, with smaller number of nodes per branch, and fewer nodes with fruits, resulting in a large reduction in coffee production. These results show the need to find an optimal tree density and management that do not compromise coffee production and protect against extreme temperatures.Recentes estudos sobre cafeeiros (Coffea arabica L. cultivados em sistemas agroflorestais no Sul do Brasil têm mostrado o potencial do sombreamento parcial no manejo desta cultura. O objectivo deste trabalho foi avaliar as condições do microclima e seus efeitos na produção de café sombreado com guandu (Cajanus cajan, em comparação ao cultivado a pleno sol, no período de maio de 2001 a agosto de 2002 em Londrina, PR. As características microclimáticas avaliadas foram: radiação global, fotossintética e saldo de radiação; temperaturas

  17. Qualidade fisiológica de sementes de feijão em plantio direto sobre diferentes coberturas de plantas em Diamantina, MG Physiological quality seeds of bean in no-tillage system over different coverings of plants in Diamantina, MG

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    Ubirajara Russi Nunes

    2007-12-01

    Full Text Available Objetivou-se com este trabalho avaliar o efeito de diferentes coberturas de plantas sobre a qualidade fisiológica de sementes de feijão em plantio direto no município de Diamantina - MG. O experimento foi instalado no Campus II da UFVJM, num Neossolo Quartzarênico Órtico típico, no delineamento em blocos casualizados, com dez tratamentos e quatro repetições. Os tratamentos constituíram-se de palhada dessecada e roçada de 1 Brachiaria decumbens cv. Basilisk, 2 Brachiaria brizantha cv. Marandu, 3 Panicum maximum cv. Tanzânia, 4 Panicum maximum cv. Mombaça, 5 Mucuna aterrina (mucuna preta, 6 Calopogonio muconoides cv. Calopogonio, 7 Dolichos lab lab (lab lab, 8 Cajanus cajan (guandu, 9 Cobertura natural e 10 Plantio convencional onde se efetuou o plantio de feijão cultivar Talismã. A qualidade fisiológica das sementes foi avaliada pelos testes de germinação (primeira contagem e contagem final; condutividade elétrica dos solutos; peso de 100 sementes; comprimento e peso das plântulas; índice de velocidade de germinação e teor de água das sementes de feijão. As gramíneas Panicum. maximum cv. Mombaça, Brachiaria brizantha cv. Marandu, B. decumbens cv. Basilisk e P. maximum cv. Tanzânia produziram matéria seca em quantidade e uniformidade para maior conservação de água e proteção do solo para viabilizar o sistema plantio direto. A qualidade das sementes não foi afetada pelos manejos de solo utilizados e sim pelas condições climáticas na maturação.The objective this work was evaluate the effect of different coverings plants on the physiological quality seeds of bean in no-tillage system in Diamantina - MG. The experiment was installed in the Campus II of UFVJM, in a Neossolo Quartzarênico Órtico típico, in blocks randomized design, with ten treatments and four replicates. The treatments were constituted of control chemical and rubbed straw of 1 Brachiaria decumbens cv. Basilisk, 2 Brachiaria brizantha cv

  18. Avaliação de um sistema de cultivo em aléias em um argissolo franco-arenoso da região amazônica Evaluation of an alley cropping system under humid tropical conditions of the amazon region

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    Emanoel Gomes de Moura

    2008-08-01

    Full Text Available No trópico úmido, a construção e manutenção da fertilidade dos solos são os maiores desafios dos que se dedicam à implantação de sistemas agrícolas sustentáveis. O objetivo deste estudo foi avaliar um sistema de cultivo em aléias com guandu, associado à adição anual de calcário e de K, em um Argissolo de textura franco-arenosa, a fim de verificar a possibilidade do uso desse sistema como alternativa ao corte e queima na agricultura do trópico úmido. Foram utilizados, como leguminosa, o guandu (Cajanus cajan e a cultura do milho. Os tratamentos foram os seguintes: T = testemunha, com solo desnudo; G2, G2,5 e G3, tratamentos com fileiras de guandu nos espaçamentos de 2, 2,5 e 3 m, respectivamente; G2K, G2,5K e G3K, tratamentos com guandu nos mesmos espaçamentos mais K; G2C, G2,5C e G3C, tratamentos com guandu mais calagem; G2KC, G2,5KC e G3KC, tratamentos com guandu mais K e calagem. A cobertura e o equilíbrio de nutrientes do solo foram os mais importantes fatores que influenciaram a produtividade do milho no sistema de cultivo em aléias com guandu; portanto, eles devem ser considerados como fundamentais para o manejo sustentável dos Argissolos de textura franco-argilosa do trópico úmido.In the humid tropics the buildup and maintenance of soil fertility are major challenges in terms of sustainability of agroecosystems. The objective of this study was to evaluate an alley cropping system with pigeon pea (Cajanus cajan on an Alfisol, with annual application of lime and potassium, with special concern regarding the viability of this system as an alternative to slash and burn practiced in tropical agriculture. Pigeon pea and maize (Zea mays were planted in the experiment with the following treatments: T = control, with bare soil; G2, G2.5 and G3 = treatments with pigeon pea, spacing between lines of 2, 2.5 and 3 m, respectively; G2K, G2.5K and G3K = pigeon pea, same spacing, plus potassium; G2C, G2.5C and G3C = pigeon pea

  19. Efeito de coberturas mortas vegetais sobre o desempenho da cenoura em cultivo orgânico Effect of mulch of different plant species on the performance of organically grown carrot

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    CAB Santos

    2011-03-01

    Full Text Available Avaliou-se o efeito de coberturas mortas com resíduos de diferentes espécies de plantas no rendimento da cenoura em cultivo orgânico e nos níveis de reinfestação pela vegetação espontânea. O experimento foi conduzido no Sistema Integrado de Produção Agroecológica ("Fazendinha Agroecológica Km 47", em Seropédica-RJ, empregando delineamento de blocos casualizados, com quatro tratamentos e seis repetições. Os tratamentos consistiram de coberturas dos canteiros com a biomassa aérea, seca e triturada, de capim Cameroon (Pennisetum purpureum, gliricídia (Gliricidia sepium e guandu (Cajanus cajan, além da testemunha (sem qualquer cobertura. Por ocasião da colheita da cenoura, determinou-se a produtividade e as médias em peso, comprimento e diâmetro máximo das raízes tuberosas. A reinfestação de ervas espontâneas foi estimada pelo número total de indivíduos por m², sendo as coletas realizadas aos 20, 50 e 80 dias após a semeadura. A cenoura cultivada nas parcelas tratadas com resíduos de leguminosas apresentou aumentos significativos de todas as variáveis fitotécnicas, com exceção para o comprimento médio da raiz. Nas parcelas cobertas com resíduos da gramínea, os resultados foram estatisticamente iguais aos da testemunha. A reinfestação por plantas espontâneas por unidade de área cultivada alcançou níveis da ordem de 300% superiores em parcelas sem cobertura do solo, na comparação com aquelas que receberam as palhadas.The effect of soil mulch with different plant species was evaluated in relation to the performance of organically grown carrots and to weed population levels. The experiment was carried out at the Integrated Agroecological Production System located in Seropédica, Metropolitan Region of Rio de Janeiro State (Baixada Fluminense, Brazil, using a randomized blocks design comprising four treatments replicated six times. These treatments consisted of soil mulch with chopped and dried above

  20. Capacidade competitiva do jatobá com adubos verdes, forrageiras e plantas daninhas Competitive capacity of Hymenaea courbaril with green manure, forage species and weeds

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    A.M.M Gandini

    2011-01-01

    Full Text Available O estudo do consórcio entre espécies anuais e perenes representa uma ferramenta importante no processo de implantação e manejo florestal. O objetivo deste trabalho foi avaliar a capacidade competitiva do jatobá (Hymenaea courbaril com espécies de adubos verdes, forrageiras e plantas daninhas quanto à alocação de matéria seca, área foliar e concentração de nutrientes. Foram conduzidos dois experimentos, sendo os tratamentos compostos pela combinação de mudas de jatobá, desenvolvendo-se isoladamente ou em competição com cada uma das seguintes espécies: Brachiaria humidicola, Brachiaria brizantha, Brachiaria decumbens, Panicum maximum, Cajanus cajan, Canavalia ensiformis e Mucuna aterrima (experimento 1 e Bidens pilosa, Cenchrus echinatus, Euphorbia heterophylla, Lolium multiflorum e Solanum americanum (experimento 2, mais o cultivo de cada planta daninha e consorte isolada. Após convivência por 60 dias, as plantas foram coletadas para avaliação de matéria seca, área foliar e teor de nutrientes. Observou-se que a competição entre as plantas não promoveu alterações na produção de matéria seca ou área foliar do jatobá. Tendo em vista o exposto, verifica-se que a capacidade competitiva do jatobá não é afetada pela presença das espécies de adubos verdes e forrageiras, possibilitando convivência em fase inicial de desenvolvimento. Quanto à convivência das plantas daninhas com o jatobá, observou-se efeito positivo no acúmulo de nutrientes por estas.The study of intercropping between annual and perennial plants is an important tool in the process of forest implantation and management. The objective of this study was to assess the competitive capacity of jatoba (Hymeneae courbaril against green manure, forage and weed species, regarding the allocation of dry matter, leaf area and nutritional content. Two experiments were installed and the treatments consisted of combinations of jatoba seedlings per plot, alone

  1. Coberturas vegetais, doses de nitrogênio e inoculação de sementes com Azospirillum brasilense em arroz de terras altas no sistema plantio direto Cover crops, nitrogen rates and seeds inoculation with Azospirillum brasilense in upland rice under no-tillage

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    Douglas de Castilho Gitti

    2013-01-01

    Full Text Available O arroz é importante fonte de energia e proteínas para a população mundial, principalmente na Ásia e Oceania. No Brasil, juntamente com o feijão, constitui a base da alimentação. Tecnologias sustentáveis que reduzam custos da produção e aumentem a produtividade do arroz podem garantir seu suprimento em períodos de alta demanda. O objetivo deste trabalho foi avaliar diferentes coberturas vegetais antecessoras (milheto [Pennisetum americanum], crotalária [Crotalaria juncea], guandu [Cajanus cajan], braquiária [Brachiaria ruziziensis], milheto + crotalária e milheto + guandu, doses de nitrogênio (N em cobertura (0, 40, 80 e 120 kg ha-1 e o efeito da inoculação de sementes com Azospirillum brasilense no arroz de terras altas em sistema plantio direto no desenvolvimento e na produtividade. Utilizou-se o delineamento experimental em blocos casualizados em esquema fatorial 6x4x2 com quatro repetições. O estudo foi desenvolvido em um Latossolo Vermelho, em Selvíria, Estado do Mato Grosso do Sul, Brasil, em 2011/2012. O cultivo do guandu antecedendo o arroz proporcionou maior produtividade do arroz somente em comparação a B. ruziziensi. A produtividade do arroz em função das doses de N em cobertura se ajustou a uma função quadrática. Não houve influência da inoculação de sementes com A. brasilense sobre a produtividade do arroz, porém houve interação entre a inoculação e as coberturas vegetais sobre o teor de N foliar, número de panículas por m², matéria seca de plantas de arroz e a massa de cem grãos.Rice is an important source of energy and protein for the world population, mainly in Asia and Oceania. In Brazil, together with common beans, it is the population's basic food. Sustainable technologies that reduce rice production costs and increase productivity can warrant its supply on period of high demand. The objective of this study was to evaluate different preceding cover crops (millet [Pennisetum americanum

  2. Influence of heat processing on the bioaccessibility of zinc and iron from cereals and pulses consumed in India.

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    Hemalatha, Sreeramaiah; Platel, Kalpana; Srinivasan, Krishnapura

    2007-01-01

    Influence of heat processing on the bioaccessibility of zinc and iron from food grains consumed in India was evaluated. Cereals - rice (Oryza sativa), finger millet (Eleusine coracana), sorghum (Sorghum vulgare), wheat (Triticum aestivum), and maize (Zea mays), and pulses - chickpea (Cicer arietinum) - whole and decorticated, green gram (Phaseolus aureus) - whole and decorticated, decorticated black gram (Phaseolus mungo), decorticated red gram (Cajanus cajan), cowpea (Vigna catjang), and French bean (Phaseolus vulgaris) were examined for zinc and iron bioaccessibility by employing an in vitro dialysability procedure. Both pressure-cooking and microwave heating were tested for their influence on mineral bioaccessibility. Zinc bioaccessibility from food grains was considerably reduced upon pressure-cooking, especially in pulses. Among cereals, pressure-cooking decreased zinc bioaccessibility by 63% and 57% in finger millet and rice, respectively. All the pressure-cooked cereals showed similar percent zinc bioaccessibility with the exception of finger millet. Bioaccessibility of zinc from pulses was generally lower as a result of pressure-cooking or microwave heating. The decrease in bioaccessibility of zinc caused by microwave heating ranged from 11.4% in chickpea (whole) to 63% in cowpea. Decrease in zinc bioaccessibility was 48% in pressure-cooked whole chickpea, 45% and 55% in pressure-cooked or microwave-heated whole green gram, 32% and 22% in pressure-cooked or microwave-heated decorticated green gram, and 45% in microwave-heated black gram. Iron bioaccessibility, on the other hand, was significantly enhanced generally from all the food grains studied upon heat treatment. Thus, heat treatment of grains produced contrasting effect on zinc and iron bioaccessibility.

  3. SOBREVIVÊNCIA E CRESCIMENTO DE SETE ESPÉCIES ARBÓREAS NATIVAS EM UMA ÁREA DEGRADADA DE FLORESTA ESTACIONAL DECIDUAL, NORTE DE MINAS GERAIS1

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    Yule Roberta Ferreira Nunes

    2015-10-01

    Full Text Available RESUMO Este trabalho objetivou avaliar o crescimento e sobrevivência de sete espécies arbóreas nativas em diferentes tratamentos, em uma área degradada na Reserva da COPASA (Juramento, MG. Para tal, foram utilizados aproximadamente 1,2 ha da área, onde foram plantadas 899 mudas, de sete espécies nativas (Anadenanthera colubrina, Copaifera langsdorffii, Dilodendron bipinatum, Myracrodruon urundeuva, Pterogyne nitens, Schinopsis brasiliensis e Senegalia polyphylla, acompanhadas durante 24 meses. A área foi dividida em cinco parcelas de 0,24 ha, que representaram os seguintes tratamentos: (T1 parcela semeada com capim (Brachiaria sp.; (T2 parcela onde as covas foram tratadas com condicionador de solo; (T3 parcela semeada com capim consorciado com leguminosa (Cajanus cajan; (T4 parcela semeada com leguminosa; e (T5 controle. A porcentagem de mortalidade foi maior no T2 e menor no T3, sendo as espécies C. langsdorffii (43,66% e S. brasiliensis (11,64% aquelas com maior e menor porcentagem de mortalidade, respectivamente. O crescimento (altura e diâmetro das mudas foi maior no T2 (24,32 ± 26,05 cm e 0,51 ± 0,37 mm, respectivamente e menor no T1 (10,82 ± 22,57 cm e 0,26 ± 0,27 mm; respectivamente. As espécies com maior crescimento em altura foram A. colubrina e S. polyphylla, já D. bipinatum e S. brasiliensis apresentaram maior crescimento em diâmetro. Pterogyne nitens apresentou o menor crescimento, além de alta mortalidade, mostrando baixa capacidade de estabelecimento em ambientes degradados.

  4. Use of plants in oral health care by the population of Mahajanga, Madagascar.

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    Ranjarisoa, Lala Nirina; Razanamihaja, Noëline; Rafatro, Herintsoa

    2016-12-04

    The use of medicinal plants to address oral health problems is not well documented in Madagascar, yet the country is full of endemic flora. The aim of this study was to collect information on the use of plants in the region of Mahajanga, Madagascar, for the treatments of oral diseases mainly tooth decay. The ethnobotanical survey with respect to the use of plants for curing dental problems was carried out in 2012. A cluster sampling at three levels was applied when choosing the study sites. The target population was made up of heads of household. The following data were collected from a semi-structured questionnaire: name of plants, part used, mode of preparation, and administration. The Informant Consensus Factor and Fidelity Level indexes were calculated for each condition treated and used plants. The Results revealed that 93 per cent of the targeted population has used plants to calm dental pain, whereas 44.2% have reported using plants due to financial problems. About 65 species of plants are commonly used for oral health care and 63 of them treated caries. Cajanus cajan (L.) Millsp. was the most plant used. It was mostly used in crushed form of 5 to 9 leaves which were prepared and placed directly on the affected oral part or in the tooth cavity. In general, the treatment lasted about 5 days or minus. The ICF were 0.83 for caries and 0.81 for periodontal diseases. This ethnobotanical survey will serve as database for further phytochemical and pharmacological study of plants in order to identify their active components and advise the population on the most effective administration. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  5. Nutrientes minerais na biomassa da parte aérea em culturas de cobertura de solo

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    Borkert Clóvis Manuel

    2003-01-01

    Full Text Available O objetivo deste trabalho foi estimar as quantidades de nutrientes reciclados por cinco espécies vegetais utilizadas como culturas de cobertura do solo e que podem retornar ao solo pela mineralização da biomassa. Foram coletadas de vários experimentos amostras da matéria verde de aveia-preta (Avena strigosa Schreb, mucu-napreta (Stizolobium aterrimum Piper & Tracy, guandu (Cajanus cajan (L. Millsp, tremoço (Lupinus albus L. e L. angustifolius L. e ervilhaca (Vicia sativa L.. Foi estimado o rendimento de matéria seca e determinados os teores de N, P, K, Ca, Mg, Mn, Zn, Cu, e, a partir dessas concentrações, foram calculadas a média observada, a média estimada e o intervalo de confiança a 95% para cada nutriente dentro de cada classe de rendimento de matéria seca, em cada espécie vegetal. Os dados foram tabulados dentro de intervalos de classe de rendimento de matéria seca e apresentadas as quantidades estimadas de nutrientes minerais. Foram ajustadas equações para estimar as quantidades desses nutrientes. A aveia-preta e a ervilhaca reciclam grande quantidade de K, e a ervilhaca, a mucu-napreta, o tremoço e o guandu reciclam grande quantidade de N. Todas as espécies reciclam quantidades apreciáveis de Ca, Mg e micronutrientes, porém baixas quantidades de P. A rotação de culturas é um meio de implementar com sucesso o aumento das áreas de lavoura em semeadura direta.

  6. Produção e comportamento reológico de exopolissacarídeos sintetizados por rizóbios isolados de guandu

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    Paulo Ivan Fernandes Júnior

    2010-12-01

    Full Text Available O objetivo deste trabalho foi avaliar a produção e o comportamento reológico de exopolissacarídeos (EPS produzidos por rizóbios isolados de nódulos de guandu [Cajanus cajan (L. Millsp.], e a similaridade desses isolados, pela técnica de análise de restrição do DNA ribossomal amplificado. As bactérias foram cultivadas em meio YM líquido e os EPS foram obtidos por precipitação com etanol gelado a partir do caldo de cultivo. Em seguida, eles foram recuperados por centrifugação, secados a vácuo, pesados e ressuspensos em água para avaliações reológicas. Os três isolados avaliados apresentaram diferenças na produção e na eficiência relativa da produção de EPS, com destaque para o isolado 8.1c. Os EPS demonstraram comportamento não newtoniano e pseudoplástico, porém também apresentaram diferenças na viscosidade aparente em uma mesma taxa de cisalhamento. Na taxa de cisalhamento de 1 s-1, os três isolados foram diferentes, enquanto na taxa de cisalhamento de 40 s-1, os isolados 53.5 e 30.6a2 foram iguais e diferiram do isolado 8.1c. A similaridade dos isolados foi condizente com os resultados de reologia dos EPS, em que bactérias que sintetizaram EPS com menor viscosidade aparente apresentaram maior similaridade

  7. Cajaninstilbene Acid Prevents Corticosterone-Induced Apoptosis in PC12 Cells by Inhibiting the Mitochondrial Apoptotic Pathway

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    Bao-Ping Jiang

    2014-09-01

    Full Text Available Background/Aims: Cajaninstilbene acid (3-hydroxy-4-prenyl-5-methoxystilben-2 -carboxylic acid, CSA, a natural stilbene isolated from the leaves of Cajanus cajan, has attracted considerable attention for its wide range of pharmacological activities. This study investigated whether CSA protects against corticosterone (CORT-induced injury in PC12 cells and examined the potential mechanisms underlying this protective effect. Methods: Cell viability and cytotoxicity were detected using a 3-(4,5-desethyithiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT assay and a lactate dehydrogenase (LDH assay kit, respectively. PC12 cell apoptosis was measured using Hoechst 33342 staining and a DNA fragmentation assay kit, and intracellular Ca2+ concentrations were assessed by fluorescent labelling. Next, the mitochondrial permeability transition pores (mPTPs and mitochondrial membrane potentials (∆Ψm were detected using a colorimetric mPTP detection kit and a 5,5',6,6'-tetrachloro-1,1',3,3'- tetraethylbenzimidazolyl-carbocyanine iodide (JC-1 kit, respectively. Finally, cytochrome c, caspase-3 and inhibitor of caspase-activated deoxyribonuclease (ICAD expression levels were monitored by western blot analysis. Results: Treatment with 100 µmol/l CORT induced cytotoxicity in PC12 cells. However, CSA dose-dependently increased cell viability and decreased LDH release as well as CORT-induced apoptosis. Mechanistically, compared with the CORT-treated group, CSA strongly attenuated intracellular Ca2+ overload and restored mitochondrial functions, including mPTPs and ∆Ψm. Furthermore, the down-regulation of cytochrome c and ICAD protein expression and the blockage of caspase-3 activity were observed upon CSA treatment. Conclusions: In summary, our data are the first to show that the in vitro antidepressant-like effect of CSA may be attributed to the cytoprotection of neurons and that such neuroprotective mechanisms are correlated with intracellular Ca2+ homeostasis

  8. Economic Viability of Small Scale Organic Production of Rice, Common Bean and Maize in Goias State, Brazil

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    Alcido Elenor Wander

    2007-04-01

    Full Text Available This study was conducted to assess the economic feasibility of small scale organic production of rice, common bean and maize in Goias State, Brazil. During 2004/05 and 2005/06 growing seasons, rice, common bean and maize were produced at the organic farm of Embrapa Rice and Beans in five mulching systems (fallow, Crotalaria juncea, Cajanus cajan, Mucuna aterrima and Sorghum bicolor , with and without tillage. Soil tillage consisted of heavy disc harrowing followed by light disc harrowing. All operations and used inputs were recorded. Based on those records, the production costs for each crop were estimated for each cropping season. The costs included operations like sowing, ploughing, harrowing, spraying, fertilizer broadcasting and harvesting, as well as inputs like seeds, inoculant strains of Rhizobium, neem oil and organic fertilizers. The benefits include the gross revenue obtained by multiplying the production amount with the market price for non-organic products. For the purpose of analysis of competitiveness of organic production in comparison to conventional farming the market prices assumed were those of conventional production. In the analysis, the costs of certification were not considered yet due to lack of certifiers in the region. For comparison between traits, net revenue, the benefit-cost-ratio (BCR and the break even point were used. In 2004/05 growing season the BCR varied from 0.27 for common bean on S. bicolor mulch system with tillage up to 4.05 for green harvested maize produced after C. juncea in no tillage system. Common bean and rice were not economically viable in this growing season. In 2005/06 growing season the BCR varied between 0.75 for common bean after S. bicolor in tillage system and 4.50 for green harvested maize produced after fallow in no tillage system. In this season common bean was economically viable in leguminous mulching systems and green harvested maize was viable in all mulching systems.

  9. Atributos físicos e estoque de carbono do solo em áreas de Terra Preta Arqueológica da Amazônia

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    José Maurício da Cunha

    2017-03-01

    Full Text Available Para se avaliar a qualidade do solo e a sustentabilidade em ambientes de Terra Preta Arqueológica da Amazônia, é importante a caracterização dos impactos de uso de solo dos diferentes tipos de cobertura. Sendo assim, o objetivo deste trabalho foi avaliar os atributos físicos e o carbono orgânico em áreas de Terra Preta Arqueológica, no município de Novo Aripuanã, AM, sob os cultivos feijão guandu (Cajanus cajan e pastagem, em comparação com a cobertura de floresta, com base em técnicas de estatística tradicional univariada e multivariada e de geoestatística. Para isso foram delimitadas malhas com espaçamentos regulares e 88 pontos amostrais georreferenciados por malha. Foram coletadas amostras em blocos de solo com estrutura preservada e anéis volumétricos, para a determinação das propriedades físicas e do carbono orgânico do solo. O feijão guandu e pastagem diferem estatisticamente da floresta, que apresentaram características de estabilidade dos agregados acima da média, mas características texturais e carbono orgânico abaixo da média. O modelo exponencial, pelo semivariograma escalonado, mostrou que atributos físicos do solo apresentaram predominância, com grau de dependência de forte a moderado, sendo a área sob feijão guandu com maior variabilidade dos atributos do solo.

  10. Chemical composition and ruminal degradation kinetics of crude protein and amino acids, and intestinal digestibility of amino acids from tropical forages

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    Lidia Ferreira Miranda

    2012-03-01

    Full Text Available The objective of this research was to determine the chemical composition and ruminal degradation of the crude protein (CP, total and individual amino acids of leaves from tropical forages: perennial soybean (Neonotonia wightii, cassava (Manihot esculenta, leucaena (Leucaena leucocephala and ramie (Boehmeria nivea, and to estimate the intestinal digestibility of the rumen undegradable protein (RUDP and individual amino acids of leaves from the tropical forages above cited, but including pigeon pea (Cajanus cajan. Three nonlactating Holstein cows were used to determine the in situ ruminal degradability of protein and amino acids from leaves (6, 18 and 48 hours of ruminal incubation. For determination of the intestinal digestibility of RUDP, the residue from ruminal incubation of the materials was used for 18 hours. A larger concentration of total amino acids for ramie and smaller for perennial soybean were observed; however, they were very similar in leucaena and cassava. Leucine was the essential amino acid of greater concentration, with the exception of cassava, which exhibited a leucine concentration 40.45% smaller. Ramie showed 14.35 and 22.31% more lysine and methionine, respectively. The intestinal digestibility of RUDP varied from 23.56; 47.87; 23.48; 25.69 and 10.86% for leucaena, perennial soybean, cassava, ramie and pigeon pea, respectively. The individual amino acids of tropical forage disappeared in different extensions in the rumen. For the correct evaluation of those forages, one should consider their composition of amino acids, degradations and intestinal digestibility, once the amino acid composition of the forage does not reflect the amino acid profiles that arrived in the small intestine. Differences between the degradation curves of CP and amino acids indicate that degradation of amino acids cannot be estimated through the degradation curve of CP, and that amino acids are not degraded in a similar degradation profile.

  11. Ganancia de peso de ovinos alimentados con pasto Taiwán (Pennisetum purpureum suplementados con diversas fuentes de proteína

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    R. González-Garduño

    2011-01-01

    Full Text Available El objetivo fue conocer la ganancia diaria de peso (gdp y estimar el consumo de pasto Taiwán en ovinos suplementados con diferentes fuentes de proteína. Se realizaron tres estudios, en el primero, se utilizó pasta de coco (pcoco (Cocus nucifera y pcoco + bloques multinutricionales (bmn que se proporcionaron a siete corderos, respectivamente, en el segundo, se proporcionó un alimento balanceado comercial a un grupo de ocho corderos y se suplementó con pcoco a otros ocho animales, y en el tercero, se suplementó con chícharo Gandul (cgandul (Cajanus cajan a cuatro hembras y cuatro machos y con pcoco a otros dos grupos similares. La investigación se realizó en Salto de Agua, Chiapas, México. Se registró el consumo diario por grupo y cada quince días se registró el peso vivo. El análisis estadístico de gdp se realizó con el procedimiento mixed del sas. Las gdp fueron mayores en el grupo con bmn (0.082± 0.06 kg respecto al sin el bmn (0.045 ± 0.09 kg. La gdp de los corderos alimentados con pcoco fue 0.09 ± 0.02 Kg y los alimentados con pasto Taiwán + pcoco tuvieron 0.06 ± 0.03 Kg. Los corderos machos suplementados con pcoco tuvieron mejor gdp (0.09 ± 0.03 Kg que machos y hembras suplementados con cgandul (0.03 ± 0.02 y 0.04 ± 0.02 Kg, respectivamente. El consumo se incrementó con la edad de los animales y mostró gran variación entre días. La suplementación con pcoco y bmn permiten incrementar las ganancias de peso de corderos estabulados.

  12. Comparative sequence analysis of nitrogen fixation-related genes in six legumes

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    Dong Hyun eKim

    2013-08-01

    Full Text Available Legumes play an important role as food and forage crops in international agriculture especially in developing countries. Legumes have a unique biological process called nitrogen fixation (NF by which they convert atmospheric nitrogen to ammonia. Although legume genomes have undergone polyploidization, duplication and divergence, NF-related genes, because of their essential functional role for legumes, might have remained conserved. To understand the relationship of divergence and evolutionary processes in legumes, this study analyzes orthologs and paralogs for selected 20 NF-related genes by using comparative genomic approaches in six legumes i.e. Medicago truncatula (Mt, Cicer arietinum, Lotus japonicus, Cajanus cajan (Cc, Phaseolus vulgaris (Pv and Glycine max (Gm. Subsequently, sequence distances, numbers of synonymous substitutions per synonymous site (Ks and nonsynonymous substitutions per nonsynonymous site (Ka between orthologs and paralogs were calculated and compared across legumes. These analyses suggest the closest relationship between Gm and Cc and the farthest distance between Mt and Pv in 6 legumes. Ks proportional plots clearly showed ancient genome duplication in all legumes, whole genome duplication event in Gm and also speciation pattern in different legumes. This study also reported some interesting observations e.g. no peak at Ks 0.4 in Gm-Gm, location of two independent genes next to each other in Mt and low Ks values for outparalogs for three genes as compared to other 12 genes. In summary, this study underlines the importance of NF-related genes and provides important insights in genome organization and evolutionary aspects of six legume species analyzed.

  13. Evolution and structural diversification of Nictaba-like lectin genes in food crops with a focus on soybean (Glycine max).

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    Van Holle, Sofie; Rougé, Pierre; Van Damme, Els J M

    2017-03-01

    The Nictaba family groups all proteins that show homology to Nictaba, the tobacco lectin. So far, Nictaba and an Arabidopsis thaliana homologue have been shown to be implicated in the plant stress response. The availability of more than 50 sequenced plant genomes provided the opportunity for a genome-wide identification of Nictaba -like genes in 15 species, representing members of the Fabaceae, Poaceae, Solanaceae, Musaceae, Arecaceae, Malvaceae and Rubiaceae. Additionally, phylogenetic relationships between the different species were explored. Furthermore, this study included domain organization analysis, searching for orthologous genes in the legume family and transcript profiling of the Nictaba -like lectin genes in soybean. Using a combination of BLASTp, InterPro analysis and hidden Markov models, the genomes of Medicago truncatula , Cicer arietinum , Lotus japonicus , Glycine max , Cajanus cajan , Phaseolus vulgaris , Theobroma cacao , Solanum lycopersicum , Solanum tuberosum , Coffea canephora , Oryza sativa , Zea mays, Sorghum bicolor , Musa acuminata and Elaeis guineensis were searched for Nictaba -like genes. Phylogenetic analysis was performed using RAxML and additional protein domains in the Nictaba-like sequences were identified using InterPro. Expression analysis of the soybean Nictaba -like genes was investigated using microarray data. Nictaba -like genes were identified in all studied species and analysis of the duplication events demonstrated that both tandem and segmental duplication contributed to the expansion of the Nictaba gene family in angiosperms. The single-domain Nictaba protein and the multi-domain F-box Nictaba architectures are ubiquitous among all analysed species and microarray analysis revealed differential expression patterns for all soybean Nictaba-like genes. Taken together, the comparative genomics data contributes to our understanding of the Nictaba -like gene family in species for which the occurrence of Nictaba domains had not

  14. Ensifer shofinae sp. nov., a novel rhizobial species isolated from root nodules of soybean (Glycine max).

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    Chen, Wen Hao; Yang, Sheng Hui; Li, Zhao Hu; Zhang, Xiao Xia; Sui, Xin Hua; Wang, En Tao; Chen, Wen Xin; Chen, Wen Feng

    2017-04-01

    Two bacterial strains isolated from root nodules of soybean were characterized phylogenetically as members of a distinct group in the genus Ensifer based on 16S rRNA gene comparisons. They were also verified as a separated group by the concatenated sequence analyses of recA, atpD and glnII (with similarities ≤93.9% to the type strains for defined species), and by the average nucleotide identities (ANI) between the whole genome sequence of the representative strain CCBAU 251167(T) and those of the closely related strains in Ensifer glycinis and Ensifer fredii (90.5% and 90.3%, respectively). Phylogeny of symbiotic genes (nodC and nifH) grouped these two strains together with some soybean-nodulating strains of E. fredii, E. glycinis and Ensifer sojae. Nodulation tests indicated that the representative strain CCBAU 251167(T) could form root nodules with capability of nitrogen fixing on its host plant and Glycine soja, Cajanus cajan, Vigna unguiculata, Phaseolus vulgaris and Astragalus membranaceus, and it formed ineffective nodules on Leucaena leucocephala. Strain CCBAU 251167(T) contained fatty acids 18:1 ω9c, 18:0 iso and 20:0, differing from other related strains. Utilization of l-threonine and d-serine as carbon source, growth at pH 6.0 and intolerance of 1% (w/v) NaCl distinguished strain CCBAU 251167(T) from other type strains of the related species. The genome size of CCBAU 251167(T) was 6.2Mbp, comprising 7,581 predicted genes with DNA G+C content of 59.9mol% and 970 unique genes. Therefore, a novel species, Ensifer shofinae sp. nov., is proposed, with CCBAU 251167(T) (=ACCC 19939(T)=LMG 29645(T)) as type strain. Copyright © 2017 Elsevier GmbH. All rights reserved.

  15. Ensifer glycinis sp. nov., a rhizobial species associated with species of the genus Glycine.

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    Yan, Hui; Yan, Jun; Sui, Xin Hua; Wang, En Tao; Chen, Wen Xin; Zhang, Xiao Xia; Chen, Wen Feng

    2016-09-01

    Rhizobial strains from root nodules of Astragalus mongholicus and soybean (Glycine max) were characterized phylogenetically as members of the genus Ensifer (formerly named Sinorhizobium), based on 16S rRNA gene sequence comparisons. Results based upon concatenated sequence analysis of three housekeeping genes (recA, atpD and glnII, ≤ 93.8 % similarities to known species) and average nucleotide identity (ANI) values of whole genome sequence comparisons (ranging from 89.6 % to 83.4 % to Ensifer fredii and Ensifer saheli, respectively) indicated the distinct positions of these novel strains within the genus Ensifer. Phylogeny of symbiotic genes (nodC and nifH) of three novel strains clustered them with rhizobial species Ensifer fredii and Ensifer sojae, both isolated from nodules of Glycine max. Cross-nodulation tests showed that the representative strain CCBAU 23380T could form root nodules with nitrogen fixation capability on Glycine soja, Albizia julibrissin, Vigna unguiculata and Cajanus cajan, but failed to nodulate Astragalus mongholicus, its original host legume. Strain CCBAU 23380T formed inefficient nodules on G. max, and it did not contain 18 : 0, 18 : 1ω7c 11-methyl or summed feature 1 fatty acids, which differed from other related strains. Failure to utilize malonic acid as a carbon source distinguished strain CCBAU 23380T from the type strains of related species. The genome size of CCBAU 23380T was 6.0 Mbp, comprising 5624 predicted genes with DNA G+C content of 62.4 mol%. Based on the results above, a novel species, Ensifer glycinis sp. nov., is proposed, with CCBAU 23380T (=LMG 29231T =HAMBI 3645T) as the type strain.

  16. Seleção de rizóbios nativos para guandu, caupi e feijão-de-porco nos tabuleiros costeiros de Sergipe

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    Fernandes Marcelo Ferreira

    2003-01-01

    Full Text Available A inoculação de estirpes de rizóbios em sementes de feijão-de-porco (Canavalia ensiformis, caupi (Vigna unguiculata e guandu (Cajanus cajan, recomendadas para outras regiões do País, não tem resultado em incrementos nas taxas de fixação biológica de N2 nem no crescimento dessas leguminosas em solos dos tabuleiros costeiros de Sergipe. Os objetivos deste trabalho foram avaliar a eficiência simbiótica de rizóbios nativos dos tabuleiros costeiros associados a essas leguminosas e a tolerância deles a estresses. Das 17 estirpes de rizóbios isoladas e analisadas em casa de vegetação, quatro foram selecionadas para o guandu, sete para o caupi e três para o feijão-de-porco. O número e a massa de nódulos secos por planta correlacionaram-se com a massa da parte aérea seca, a área foliar e o N total acumulado nas folhas das três leguminosas. Os mesmos rizóbios foram eficientes para o caupi e para o guandu. Três estirpes do guandu (R35, R43 e R45 e duas do caupi (R10 e R17 foram caracterizadas in vitro e todas apresentaram tolerância às concentrações elevadas de ácido nalidíxico, cloranfenicol e tetraciclina, porém, foram sensíveis à estreptomicina e à kanamicina. Todas as estirpes cresceram a 35masculineC e, exceto a R17, toleraram o alumínio (10 mg L-1.

  17. ADUBOS VERDES NA FITORREMEDIAÇÃO DE SOLOS CONTAMINADOS COM O HERBICIDA TEBUTHIURON

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    Fábio Ribeiro Pires

    2006-01-01

    Full Text Available The tebuthiuron residue, used in the sugar-cane culture, can be found in soil until two or more years after its application. Recent researches are being done using the phytoremediation in the attempt of removing it from the soil. The purpose of this work was to evaluate the effectiveness of the green manure use in the phyitoremediation of the tebuthiuron herbicide. The evaluated species were: Cajanus cajan, Canavalia ensiformis, Dolichos lablab, Pennisetum glaucum, Estizolobium deeringianum, Estizolobium aterrimum and Lupinus albus. These were sown and cultivated in pots containing soil treated with different doses of tebuthiuron (0.0; 0.5; 1.0; and 1.5 kg.ha-1. A control treatment without green manure, submitted to the same dosages, was kept. Sixty days after planting, green manure aerial part of all plants were harvested and soybean was sowed in the same pot, to bioassay accomplishment. Sixty days after sowing, the soybean plants were harvested, being the following evaluations carried out: height of plants, phytotoxicity symptoms and plants above ground dry biomass and leaf area. Until 0.5 kg.ha-1 tebuthiuron dosage, the species that better phytoremediated this herbicide was L. albus. When the soil was treated with tebuthiuron at 1.0 kg.ha-1, the C. ensiformis, followed by L. albus and S. aterrimum, they were the treatments that better phytoremediated tebuthiuron because results in highest plant height, above ground dry biomass and lesser phytotoxicity symptoms and still the biggest foliar area of the soybean plants. When the tebuthiuron was applied at 1.5 kg ha-1 dosage, it was impossible to evaluate the phyitoremediation, as the tested plants were eliminated.

  18. Efeito da suplementação com feno de Leucena (Leucaena leucocephala (Lam de Wit durante a estação seca sobre o desenvolvimento ponderal de ovinos Effect of supplementation with Leucaena leucocephala hay during the dry season on the ponderal development sheep

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    Antônio Alves de Souza

    1999-01-01

    Full Text Available O objetivo deste experimento foi estudar o desempenho de borregos suplementados com dois níveis de feno de leucena (Leucaena leucocephala (Lam de Wit, durante a estação seca, em comparação com animais mantidos em pastagem exclusiva de capim-buffel (Cenchrus ciliaris, L ou em pastagem consorciada de capim-buffel com guandu (Cajanus cajan. Durante a estação das águas, foi determinado ainda o desempenho dos animais que foram mantidos em pastagem comum de capim-buffel, sem suplementação. Os seguintes tratamentos foram testados durante a estação seca: A - pasto de capim-buffel; B - pasto de capim-buffel consorciado com guandu; C - pasto de capim-buffel suplementado com feno de leucena (250 g/animal•d; e D - pasto de capim-buffel suplementado com feno de leucena (500 g/animal•d. Durante a estação seca, somente os borregos suplementados com o nível mais alto de feno de leucena (tratamento D apresentaram ganho diário de peso mais elevado que os animais mantidos em dieta exclusiva de pasto (tratamentos A e B. Na estação das águas, os animais que apresentaram melhor desempenho durante a estação seca passaram a apresentar ganho diário de peso inferior em relação aos dos demais tratamentos. Quando as duas estações foram consideradas em conjunto, a vantagem com a suplementação desapareceu e não houve diferenças entre tratamentos. O uso de feno de leucena para suplementação de borregos mantidos a pasto, durante a estação seca, é eficiente, desde que os animais estejam terminados e sejam abatidos ao final da estação.

  19. Legumes and forage species sole or intercropped with corn in soybean-corn succession in midwestern Brazil

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    Gessí Ceccon

    2013-02-01

    Full Text Available The feasibility of no-tillage in the Cerrado (Savanna-like vegetation of Brazil depends on the production of sufficient above-ground crop residue, which can be increased by corn-forage intercropping. This study evaluated how above-ground crop residue production and yields of soybean and late-season corn in a soybean-corn rotation were influenced by the following crops in the year before soybean: corn (Zea mays L. intercropped with Brachiaria (Urochloa brizantha cv. Marandu, B. decumbens cv. Basilisk, B. ruziziensis, cv. comum., Panicummaximum cv. Tanzânia, sunn hemp (Crotalaria juncea L., pigeon pea [Cajanus cajan (L. Millsp]; sole corn, forage sorghum [Sorghum bicolor (L. Moench (cv. Santa Elisa], and ruzi grass. In March 2005, corn and forage species were planted in alternate rows spaced 0.90 m apart, and sole forage species were planted in rows spaced 0.45 m apart. In October 2005, the forages were killed with glyphosate and soybean was planted. After the soybean harvest in March 2006, sole late-season corn was planted in the entire experimental area. Corn grain and stover yields were unaffected by intercropping. Above-ground crop residue was greater when corn was intercropped with Tanzania grass (10.7 Mg ha-1, Marandu (10.1 Mg ha-1, and Ruzi Grass (9.8 Mg ha-1 than when corn was not intercropped (4.0 Mg ha-1. The intercropped treatments increased the percentage of soil surface covered with crop residue. Soybean and corn grain yields were higher after sole ruzi grass and intercropped ruzi grass than after other crops. The intercropping corn with Brachiaria spp. and corn with Panicum spp. increases above-ground crop residue production and maintains nutrients in the soil without reducing late-season corn yield and the viability of no-till in the midwestern region of Brazil.

  20. Cajaninstilbene acid relaxes rat renal arteries: roles of Ca2+ antagonism and protein kinase C-dependent mechanism.

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    Dong-Mei Zhang

    Full Text Available Cajaninstilbene acid (CSA is a major active component present in the leaves of Cajanus cajan (L. Millsp. The present study explores the underlying cellular mechanisms for CSA-induced relaxation in rat renal arteries. Vascular reactivity was examined in arterial rings that were suspended in a Multi Myograph System and the expression of signaling proteins was assessed by Western blotting method. CSA (0.1-10 µM produced relaxations in rings pre-contracted by phenylephrine, serotonin, 9, 11-dideoxy-9α, 11α-epoxymethanoprostaglandin F(2α (U46619, and 60 mM KCl. CSA-induced relaxations did not show difference between genders and were unaffected by endothelium denudation, nor by treatment with N(G-nitro-L-arginine methyl ester, indomethacin, ICI-182780, tetraethylammonium ion, BaCl(2, glibenclamide, 4-aminopyridine or propranolol. CSA reduced contraction induced by CaCl(2 (0.01-5 mM in Ca(2+-free 60 mM KCl solution and by 30 nM (--Bay K8644 in 15 mM KCl solution. CSA inhibited 60 mM KCl-induced Ca(2+ influx in smooth muscle of renal arteries. In addition, CSA inhibited contraction evoked by phorbol 12-myristate 13-acetate (PMA, protein kinase C agonist in Ca(2+-free Krebs solution. Moreover, CSA reduced the U46619- and PMA-induced phosphorylation of myosin light chain (MLC at Ser19 and myosin phosphatase target subunit 1 (MYPT1 at Thr853 which was associated with vasoconstriction. CSA also lowered the phosphorylation of protein kinase C (PKCδ at Thr505. In summary, the present results suggest that CSA relaxes renal arteries in vitro via multiple cellular mechanisms involving partial inhibition of calcium entry via nifedipine-sensitive calcium channels, protein kinase C and Rho kinase.

  1. Remnants of the Legume Ancestral Genome Preserved in Gene-Rich Regions: Insights from Lupinus angustifolius Physical, Genetic, and Comparative Mapping.

    Science.gov (United States)

    Książkiewicz, Michał; Zielezinski, Andrzej; Wyrwa, Katarzyna; Szczepaniak, Anna; Rychel, Sandra; Karlowski, Wojciech; Wolko, Bogdan; Naganowska, Barbara

    The narrow-leafed lupin (Lupinus angustifolius) was recently considered as a legume reference species. Genetic resources have been developed, including a draft genome sequence, linkage maps, nuclear DNA libraries, and cytogenetic chromosome-specific landmarks. Here, we used a complex approach, involving DNA fingerprinting, sequencing, genetic mapping, and molecular cytogenetics, to localize and analyze L. angustifolius gene-rich regions (GRRs). A L. angustifolius genomic bacterial artificial chromosome (BAC) library was screened with short sequence repeat (SSR)-based probes. Selected BACs were fingerprinted and assembled into contigs. BAC-end sequence (BES) annotation allowed us to choose clones for sequencing, targeting GRRs. Additionally, BESs were aligned to the scaffolds of the genome sequence. The genetic map was supplemented with 35 BES-derived markers, distributed in 14 linkage groups and tagging 37 scaffolds. The identified GRRs had an average gene density of 19.6 genes/100 kb and physical-to-genetic distance ratios of 11 to 109 kb/cM. Physical and genetic mapping was supported by multi-BAC-fluorescence in situ hybridization (FISH), and five new linkage groups were assigned to the chromosomes. Syntenic links to the genome sequences of five legume species (Medicago truncatula, Glycine max, Lotus japonicus, Phaseolus vulgaris, and Cajanus cajan) were identified. The comparative mapping of the two largest lupin GRRs provides novel evidence for ancient duplications in all of the studied species. These regions are conserved among representatives of the main clades of Papilionoideae. Furthermore, despite the complex evolution of legumes, some segments of the nuclear genome were not substantially modified and retained their quasi-ancestral structures. Cytogenetic markers anchored in these regions constitute a platform for heterologous mapping of legume genomes.

  2. Aspectos agronômicos de leguminosas para adubação verde no Cerrado do Alto Vale do Jequitinhonha

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    Ricardo Borges Teodoro

    2011-04-01

    Full Text Available O uso intensivo e inadequado dos solos acelera sua degradação, sendo necessária a intervenção por meio de práticas conservacionistas para restaurar a capacidade produtiva dos mesmos. O objetivo deste estudo foi avaliar o comportamento e desenvolvimento de diferentes leguminosas utilizadas como adubos verdes em solos de Cerrado, Alto Vale do Jequitinhonha, em Turmalina, MG. O delineamento experimental adotado foi em blocos ao acaso, com sete tratamentos e quatro repetições, sendo os tratamentos constituídos pelas leguminosas: mucuna-cinza (Mucuna nivea, mucuna-preta (Mucuna aterrima, lablabe (Dolichos lablab, feijão-de-porco (Canavalia ensiformis, Crotalaria juncea, Crotalaria spectabilis e guandu-anão (Cajanus cajan. O ciclo precoce de C . juncea, C. spectabilis e feijão-de-porco favorece a inserção destes nos sistemas de cultivo. Aos 40 dias, o feijão-de-porco e mucuna-cinza já cobriam o solo, com 67 e 63 %; já o guandu-anão e C. juncea apresentaram os maiores desenvolvimentos, nesse período. Os teores de N, P e K tendem a diminuir nas avaliações realizadas nas diferentes fases vegetativas, o que contribui para melhor escolha da época de manejo das leguminosas. Crotalaria juncea, mucuna-cinza, feijão-de-porco e mucuna-preta foram as leguminosas que se destacaram na produção de matéria seca, o que torna essas espécies promissoras para adubação verde na região. As leguminosas, em sua maioria, apresentam potencial para reciclagem dos macronutrientes e aporte de N aos sistemas de produção.

  3. Methane Production of Different Forages in Ruminal Fermentation

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    S. J. Meale

    2012-01-01

    Full Text Available An in vitro rumen batch culture study was completed to compare effects of common grasses, leguminous shrubs and non-leguminous shrubs used for livestock grazing in Australia and Ghana on CH4 production and fermentation characteristics. Grass species included Andropodon gayanus, Brachiaria ruziziensis and Pennisetum purpureum. Leguminous shrub species included Cajanus cajan, Cratylia argentea, Gliricidia sepium, Leucaena leucocephala and Stylosanthes guianensis and non-leguminous shrub species included Annona senegalensis, Moringa oleifera, Securinega virosa and Vitellaria paradoxa. Leaves were harvested, dried at 55°C and ground through a 1 mm screen. Serum bottles containing 500 mg of forage, modified McDougall’s buffer and rumen fluid were incubated under anaerobic conditions at 39°C for 24 h. Samples of each forage type were removed after 0, 2, 6, 12 and 24 h of incubation for determination of cumulative gas production. Methane production, ammonia concentration and proportions of VFA were measured at 24 h. Concentration of aNDF (g/kg DM ranged from 671 to 713 (grasses, 377 to 590 (leguminous shrubs and 288 to 517 (non-leguminous shrubs. After 24 h of in vitro incubation, cumulative gas, CH4 production, ammonia concentration, proportion of propionate in VFA and IVDMD differed (p<0.05 within each forage type. B. ruziziensis and G. sepium produced the highest cumulative gas, IVDMD, total VFA, proportion of propionate in VFA and the lowest A:P ratios within their forage types. Consequently, these two species produced moderate CH4 emissions without compromising digestion. Grazing of these two species may be a strategy to reduce CH4 emissions however further assessment in in vivo trials and at different stages of maturity is recommended.

  4. EFECTO DE LA GERMINACIÓN SOBRE EL CONTENIDO DE HIERRO Y CALCIO EN AMARANTO, QUINUA, GUANDUL Y SOYA EFEITO DA GERMINAÇÃO SOBRE CONTEUDO DA FERRO E CÁLCIO PRESENTES EM QUINOA, AMARANTO, GUANDU E SOJA GERMINATION EFFECT ON IRON AND CALCIUM CONTENT IN AMARANTH, QUINOA, PIGEON PEA AND SOYBEAN

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    DIANA C CHAPARRO

    2011-06-01

    Full Text Available Se evaluaron los cambios en la cantidad de hierro y calcio durante el proceso germinativo en semillas de amaranto (Amaranthus sp, quinua (Chenopodium quinoa, guandul (Cajanus cajan y soya (Glycine maxy. Se utilizaron semillas producidas por agricultores del departamento del Cauca; las cuatro especies fueron seleccionadas para asegurar un grado óptimo en su calidad física y un porcentaje de germinación mayor al 90%. Inicialmente se estandarizó el método para la obtención de semillas germinadas, mediante la definición de variables como uso o no de desinfectante, tipo de sustrato, tiempo de germinación y temperatura de germinación. Para el desarrollo del estudio se aplicó un diseño de bloques completos al azar con tres réplicas por día de germinación (días cero, uno, dos y tres; para cuantificación de hierro y calcio se utilizó absorción atómica. Los resultados encontrados permiten concluir que la germinación indujo cambios en la disponibilidad de hierro y calcio de forma particular en la semilla de cada especie; el hierro disponible, en semillas de amaranto soya y quinua, tendió a disminuir a medida que avanzó el proceso de germinación; sin embargo, la germinación no afectó significativamente el contenido de hierro en semillas de amaranto y guandul. El calcio disponible se incrementó significativamente en semillas de amaranto y quinua a partir del segundo día de germinación, la germinación generó un incremento porcentual, en el contenido de calcio, de 169.1% en amaranto y de 24.75% en quinua, mientras que en guandul y soya el calcio disponible disminuyó con la germinación.Foram avaliadas as alterações na quantidade disponível de ferro e cálcio durante o processo de germinação das sementes de amaranto (Amaranthus sp, quinoa (Chenopodium quinoa, guandu (Cajanus cajan e soja (Glycine max y. Empregaram- se sementes produzidas por agricultores da região; as quatro espécies foram selecionadas para assegurar um

  5. EFECTO DE LA GERMINACIÓN SOBRE EL CONTENIDO Y DIGESTIBILIDAD DE PROTEÍNA EN SEMILLAS DE AMARANTO, QUINUA, SOYA Y GUANDUL EFEITO DA GERMINAÇÃO SOBRE O CONTEÚDO E DIGESTIBILIDADE DE PROTEÍNAS EM SEMENTES DE AMARANTO, QUINUA, SOJA E GUANDUL EFFECT OF THE GERMINATION ON THE PROTEIN CONTENT AND DIGESTIBILITY IN AMARANTH, QUINUA, SOY BEAN AND GUANDUL SEEDS

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    D.C. CHAPARRO ROJAS

    2010-06-01

    Full Text Available Se evaluaron los cambios en la concentración y digestibilidad de proteína durante la germinación en semillas de amaranto (Amaranthus sp, quinua (Chenopodium quinoa w., soya (Glycine max y guandul (Cajanus cajan. Las semillas utilizadas fueron suministradas por agricultores del Departamento del Cauca, seleccionadas asegurando calidad grado uno y porcentaje de germinación mayor al 90 %. Se estandarizó el método para la obtención de semillas germinadas, mediante la definición de variables como uso o no de desinfectante, tipo de sustrato, tiempo de germinación y temperatura. Se aplicó un diseño de bloques completos al azar con tres réplicas por día de germinación, para los días cero, uno, dos y tres; para cuantificación de proteína se utilizó Kjeldhal y para digestibilidad in-vitro de la proteína, se utilizó digestibilidad en pepsina. Los hallazgos permitieron concluir que la germinación induce cambios en la concentración y digestibilidad de la proteína de forma particular en cada tipo de semillas; en amaranto y soya, la germinación generó un incremento significativo en el contenido de proteína a partir del segundo día de germinación y en guandul a partir del primer día; en la quinua genero un descenso en el contenido de proteína en el segundo día, siendo estadísticamente igual en los días cero, uno y tres de germinación. La germinación mejoro la digestibilidad de la proteína, en semillas de quinua, guandul y soya, no generó cambios en semillas de amarantoSe avaliaram as mudanças na concentração e dlgistibiidade de proteína durante a germinação em sementes de amaranto (Amaranthus sp, quinua (Chenopodium quinoa w, soja (Glycine maxe guandul (Cajanus cajan. As sementes empregadas foram fornecidas por agricultores do Departamento do Cauca, selecionandoas para garantir a qualidade grau um e a porcentagem de germinação maior ao 90%. Padronizouse o método para a obtenção de sementes germinadas, mediante a

  6. Nitrogen-fixation Potential of Nodules in Four Types of Nitrogen-fixation Plants and Their Influencing Factors in Dry-hot Valley%干热河谷4种固氮植物根瘤固氮潜力及其影响因素

    Institute of Scientific and Technical Information of China (English)

    唐国勇; 李昆; 孙永玉; 张春华

    2012-01-01

    In addition to water, Nitrogen ( N) is often the key limiting factor for biological activity in Dry-hot Valleys, Biological N-fixation by nitrogen-fixation plants is of important source of N for vegetations in those areas. The nitrogenase activities (NAs) of nodules in Acacia auriliformis A. Cunn, Leucaena leucacephala (Lara. ) de Wit, Cajanus cajan ( L). Millspangh and Albiza kalkora Prain plantations were determined at the Dry red soils and Verti-sol spots at four different sampling times in a Dry-hot Valley with the acetylene reduction assay. The results showed that the NAs of nodules in L. leucacephala (16, 25 μmol·g -1·h-1 ) and A. auriliformis ( 15. 85μmol·g-1·h-1) were significantly higher than those in A. kalkora (9,60 μmol·g-1·h-1) and C. cajan (9.42 μmol·g-1h-1 )·The NAs of nodules in rainy season were significantly higher than those in dry season, and approximated 2. 3 times that in dry season. The NAs of nodules at the Dry red soils spots were 1.3 -1.6 times higher than those at the Vertisol spots. The research revealed besides plant type, the NAs of nodules were primarily affected by soil type, season and soil water content, but less affected by soil temperature.%氮是除水分之外影响干热河谷生物活性的关键因子,豆科植物生物固氮是该地区氮素的重要来源之一.采用乙炔还原法测定了干热河谷不同季节燥红土和变性土林地中大叶相思、新银合欢、木豆和山合欢根瘤固氮酶活性(NAs).结果表明:新银合欢(16.25 μmol · g-1·h-1)和大叶相思(15.85 μmol·g-1·h-1)根瘤NAs显著(P<0.001)高于山合欢(9.60 μmol·g-1·h-1)和木豆(9.42 μmol·g-1·h-1).雨季根瘤NAs显著高于旱季,约为旱季的2.3倍.燥红土样地上植物根瘤NAs是变性土样地的1.3~1.6倍.研究揭示:除植被类型外,干热河谷植物根瘤NAs主要受土壤类型、季节和土壤含水量的影响,而受土壤温度的影响较小.

  7. Efeito do uso de diferentes compostos na produção de tomate (Lycopersicum esculentum Mill em cultivo orgânico

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    M. S. Teodoro

    2015-12-01

    Full Text Available O objetivo do presente trabalho foi avaliar, em condições de casa de vegetação, a influência de cinco compostos orgânicos na adubação de plantio e de cobertura de tomate híbrido do tipo Caqui. O delineamento experimental adotado foi o de blocos casualizados, com quatro repetições. Foram avaliados cinco tratamentos, contendo biomassa vegetal provenientes do banco de leguminosas da Embrapa Meio-Norte. As leguminosas utilizadas na confecção dos compostos foram o guandu Cultivar Fava Larga (Cajanus cajan, a Crotalária juncea (Crotalaria juncea L., a Leucena (Leucaena leucocephala, a Gliricídia (Gliricidia sepium e a Mucuna preta (Mucuna aterrima. A gramínea utilizada foi o capim elefante (Pennisetum purpureum. Os frutos do tomate começaram a ser colhidos no início do amadurecimento, por volta de 70 a 120 dias após o transplantio. Avaliaram-se a produção total de frutos (PTF, produção de frutos comerciais (PFC, número total de frutos (NTF, número de frutos comerciais (NFC, peso médio de frutos comerciais (PMFC e diâmetro médio de frutos (DMF. Foram registradas ocorrências de pragas nas condições experimentais, detectando-se a presença de nematóides (Meloydogines spp e cochonilha (Maconellicoccus hirsutus. Não houve diferença significativa entre os tratamentos estudados, entretanto, o tratamento 2 foi o que se destacou quanto as variáveis analisadas, principalmente para PTF (54,5 t/ha e PFC (49,6 t/ha. Concluiu-se que o uso de compostos orgânicos com palhadas de leguminosas e gramíneas é uma alternativa técnica e economicamente viável, podendo reduzir o uso integral do esterco bovino na adubação de plantio e de cobertura do tomate produzido no sistema orgânico.Effect of the use of different compounds in the production of tomato (Lycopersicum esculentum Mill in organic farmingAbstract: - The purpose of this study was to evaluate, in greenhouse conditions, the influence of five organic compounds on fertilizing

  8. Soil Aggregate Features under Tamarindus indica Forest with Different Plant Compositions in Dry-hot Valley%干热河谷不同酸豆林土壤团聚体特征分析

    Institute of Scientific and Technical Information of China (English)

    彭辉; 周红敏; 徐肇友; 瞿虹; 纪中华

    2014-01-01

    Experiments were conducted on the soil aggregates underTamarindus indica forest with bared land(L+G)CK), with fireweed(L+Z), with Paspalum notatum (L+B), withPhyllanthus emblica (L+Y), and withCajanus cajan (L+M) in dry-hot valley of Yunnan. R0.25(percentage of soil aggregates with diameter larger than 0.25mm), mean weight diameter (MWD), geometric mean diameter (GMD) and fractal dimension (D) were determined at 3 soil layers (0 - 10 cm, >10 - 20 cm, >20 – 40 cm). The results showed that L+B promoted the conservation of soil macro-aggregates, L+Z and L+G could improve the soil aggregate structure, L+Y and L+M forestlands reduced the proportion of macro-aggregates in the soil but increased micro-aggregates content. The experiment demonstrated that in dry-hot valley, conservation tillage was conducive to the sustainable management ofT. indica.%采用干筛法对金沙江干热河谷区酸豆林+光板地(裸地)、酸豆林+杂草、酸豆林+百喜草、酸豆林+余甘子、酸豆林+木豆5种酸豆林地在0~10 cm、>10~20 cm、>20~40 cm的3个土层中的R0.25、MWD、GMD和分形维数D变化进行研究,结果表明:5种酸豆林模式中,酸豆林+百喜草模式有利于土壤大团聚体的保存,酸豆林+杂草模式和酸豆林+光板地模式提升土壤团聚结构,但是效果并不显著,而酸豆林+余甘子模式和酸豆林+木豆模式则降低了土壤中大团聚体的比例,使土壤中的微团聚体含量增加;在干热河谷实施保护性耕作有利于酸豆林的可持续经营。

  9. Caracterização genética de rizóbios nativos dos tabuleiros costeiros eficientes em culturas do guandu e caupi

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    Fernandes Marcelo Ferreira

    2003-01-01

    Full Text Available O objetivo deste trabalho foi caracterizar geneticamente sete estirpes de rizóbios nativos dos tabuleiros costeiros de Sergipe com alta eficiência de fixação biológica do N2 em associação com guandu (Cajanus cajan e caupi (Vigna unguiculata. A amplificação do DNA pela técnica de PCR (polymerase chain reaction com o oligonucleotídeo específico BOX indicou um grau elevado de diversidade genética, uma vez que todas as estirpes apresentaram perfis únicos de DNA. A análise por BOX-PCR revelou, ainda, que essa metodologia é eficiente para diferenciar estirpes, mas não para a diferenciação de espécies de rizóbio. Pela técnica do RFLP (restriction fragment length polymorphism da região do DNA que codifica o gene 16S rRNA e da região intergênica entre os genes 16S e 23S rRNA, com cinco enzimas de restrição, bem como pelo seqüenciamento parcial da região do 16S rRNA, foi possível classificar as estirpes nos gêneros Bradyrhizobium e Rhizobium. Houve coerência entre as análises envolvendo a região do 16S rRNA, mas o agrupamento com uma das estirpes diferiu pela análise do espaço intergênico. Os resultados obtidos com a estirpe R11 indicam variabilidade genética elevada em relação às espécies de rizóbios descritas, inclusive diferindo em diversas bases da região do 16S rRNA, e podem indicar uma nova espécie.

  10. Effect of cropping systems in no-till farming on the quality of a Brazilian Oxisol

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    Getulio de Freitas Seben Junior

    2014-08-01

    Full Text Available The no-till system with complex cropping sequences may improve the structural quality and carbon (C sequestration in soils of the tropics. Thus, the objective of this study was to evaluate the effects of cropping sequences after eight years under the no-till system on the physical properties and C sequestration in an Oxisol in the municipality of Jaboticabal, Sao Paulo, Brazil. A randomized split-block design with three replications was used. The treatments were combinations of three summer cropping sequences - corn/corn (Zea mays L. (CC, soybean/soybean (Glycine max L. Merryll (SS, and soybean-corn (SC; and seven winter crops - corn, sunflower (Helianthus annuus L., oilseed radish (Raphanus sativus L., pearl millet (Pennisetum americanum (L. Leeke, pigeon pea (Cajanus cajan (L. Millsp, grain sorghum (Sorghum bicolor (L. Moench, and sunn hemp (Crotalaria juncea L.. Soil samples were taken at the 0-10 cm depth after eight years of experimentation. Soil under SC and CC had higher mean weight diameter (3.63 and 3.55 mm, respectively and geometric mean diameter (3.55 and 2.92 mm of the aggregates compared to soil under SS (3.18 and 2.46 mm. The CC resulted in the highest soil organic C content (17.07 g kg-1, soil C stock (15.70 Mg ha-1, and rate of C sequestration (0.70 Mg ha-1 yr-1 among the summer crops. Among the winter crops, soil under pigeon pea had the highest total porosity (0.50 m³ m-3, and that under sunn hemp had the highest water stable aggregates (93.74 %. In addition, sunn hemp did not differ from grain sorghum and contained the highest soil organic C content (16.82 g kg-1 and also had the highest rate of C sequestration (0.67 Mg ha-1 yr-1. The soil resistance to penetration was the lower limit of the least limiting water range, while the upper limit was air-filled porosity for soil bulk densities higher than 1.39 kg dm-3 for all cropping sequences. Within the SC sequence, soil under corn and pigeon pea increased least limiting water

  11. Atributos químicos e estabilidade de agregados sob diferentes culturas de cobertura em Latossolo do cerrado Chemical properties and aggregate stability under different cover crops in cerrado Oxisol

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    Glenio G. Santos

    2012-11-01

    Full Text Available Objetivou-se, com este trabalho, avaliar o efeito de diferentes culturas de cobertura sobre os atributos químicos e a estabilidade de agregados de um Latossolo do cerrado, sob plantio direto. O estudo foi conduzido em área experimental na Embrapa Arroz e Feijão, em Santo Antônio de Goiás, GO. As culturas de cobertura avaliadas foram: braquiária, milho em consórcio com braquiária (integração lavoura-pecuária, guandu anão, milheto, capim mombaça, sorgo granífero, estilosantes e crotalária. As amostras foram coletadas em abril de 2005 e 2006. O delineamento experimental foi o de blocos completos ao acaso com quatro repetições e os tratamentos arranjados em esquema fatorial 8 x 2, sendo oito culturas de cobertura e duas profundidades de amostragem do solo: 0-0,10 e 0,10-0,20 m. As culturas de cobertura influenciam, de forma diferenciada, os valores de pH e os teores de cálcio, magnésio, alumínio, fósforo, potássio, cobre, zinco e ferro do solo. O tratamento estilosantes tem maior poder em acidificar o solo. A agregação do solo varia com as culturas de cobertura e com a profundidade.The objective of this study was to evaluate the effect of different cover crops on chemical properties and aggregate stability in a cerrado Oxisol under no-tillage. The study was carried out in Embrapa Rice and Beans, in Santo Antônio de Goiás, GO, Brazil. The cover crops evaluated were: Urochloa brizantha, Urochloa brizantha and corn in association (crop-livestock integrated, Cajanus cajan, Pennisetum glaucum, Panicum maximum, Sorghum bicolor, Stylosanthes guianensis and Crotalaria juncea. The soil samples were collected in April and September 2005 and April 2006. The experimental design was in completely randomized blocks with four replications and treatments arranged in factorial scheme 8 x 2, eight cover crops and two soil sampling depths, 0-0.10 and 0.10-0.20 m. The different cover crops affect pH values and calcium, magnesium, aluminum

  12. Nutritional characteristics of two pigeon pea hybrids – Liming and phosphated fertilization

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    Marcia Atauri Cardelli de Lucena

    2012-12-01

    Full Text Available The use of legumes in animal production systems can be a sustainable alternative as a protein source in rotational grazing system and/or as a protein bank. Pigeon pea (Cajanus cajan (L. Millsp. figure as an example of success of this use on animal nutrition. The development of this species can be limited by the high acidity and low soil phosphorus content. There is a lack of scientific information on the effects of liming and phosphorus fertilization on some nutritional variables of two pigeon pea new hybrids. This study was conducted in pots containing 5 kg of soil in a greenhouse at the Instituto de Zootecnia, Nova Odessa, São Paulo State. There were studied two pigeon pea hybrids, H1 and H2, and the treatments involved agronomic practices: 1 No liming and without phosphorus (control, 2 Liming (L, 3 Phosphorus fertilization (P and 4 Liming plus phosphorus. Liming was proposed to increase soil base saturation to 50%, it was used dolomite lime PRNT = 90%, in an amount corresponding to 4.5 t/ha. Phosphorus fertilization (as superphosphate rate was 60 kg/ha of PO25. The experimental units were allocated according to a complete randomised block design, with five replications. We analyzed the levels of crude protein (CP, neutral detergent fiber (NDF, acid detergent fiber (ADF, in pigeon pea shoot at 45 days of age. Statistical analyzes were performed using the software SISVAR, averages were compared using test for multiple comparisons Student Newman-Keuls - SNK test (P < 0.05. The H1 hybrid had the highest content of CP, by applying P, lime plus P and the control treatment compared to H2 hybrid. The association lime plus P resulted in higher content of CP mainly due to the increased availability of P for plants. Smaller values were observed for NDF in H2 with P application. Lower values of ADF were observed in H1 in both control treatment and P application. The ADF values were lower for the hybrid H2 only for the treatment lime plus P. The two

  13. Relação de atributos do solo com a agregação de um Latossolo Vermelho sob sistemas de preparo e plantas de verão para cobertura do solo Relationship of soil attributes with aggregate stability of a hapludox under distinct tillage systems and summer cover crops

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    Jackson Adriano Albuquerque

    2005-06-01

    Full Text Available O preparo de solo e as espécies vegetais que compõem o sistema de rotação de culturas têm expressivo efeito na qualidade física do solo, principalmente na estabilidade de agregados. Neste estudo, objetivou-se avaliar a estabilidade dos agregados de um solo cultivado com preparo reduzido (PR e preparo convencional (PC com utilização de plantas de cobertura. Dois experimentos foram efetuados em Chapecó (SC, num Latossolo Vermelho distroférrico em PR (1993-1998 e PC (1994-1998, nos quais foram avaliados cinco sistemas de cultura: milho + guandu anão; milho + mucuna cinza; milho + feijão-de-porco; milho + soja preta e milho isolado. O uso do solo degradou suas propriedades físicas, comparativamente ao sistema com mata nativa, reduziu o teor de C orgânico (CO e a estabilidade dos agregados. As plantas de cobertura de verão aumentaram o teor de CO; entretanto, não modificaram o grau de floculação de argila e a estabilidade de agregados, quando comparadas às do sistema milho isolado. A recuperação de solos degradados por meio do uso de plantas de cobertura foi mais efetiva quando estas foram associadas ao preparo reduzido, evidenciando a importância de sistemas de manejo com baixo revolvimento e alto aporte de resíduos vegetais para aumentar o CO do solo.Tillage and plant species that compose the soil management and crop rotation systems have a pronounced effect on soil physical quality, especially on aggregate stability. This study aimed to evaluate the degree of aggregate stability of a soil cultivated under reduced tillage (RT and conventional tillage (CT using distinct summer cover crops. Two experiments were carried out in Chapecó, State of Santa Catarina, Brazil on a Hapludox under RT (1993-1998 and CT (1994-1998. Five crop systems were evaluated in each tillage system: maize + Cajanus cajan (pigeon pea, maize + Stilozobiun niveum (mucuna, maize + Canavalia ensiformis (jack bean, maize + Glycine sp (black soybean, and

  14. Sistemas de preparo do solo e culturas de cobertura na produção orgânica de feijão e milho: I - atributos físicos do solo

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    Eurâimi de Queiroz Cunha

    2011-04-01

    Full Text Available Há necessidade de se avaliar a contribuição de plantas de cobertura e do seu manejo na manutenção ou melhoria da qualidade física do solo em áreas sob produção orgânica. Este trabalho objetivou determinar a influência das plantas de cobertura crotalária (Crotalaria juncea, guandu (Cajanus cajan (L. Millsp, mucuna-preta (Mucuna aterrima, sorgo-vassoura (Sorgum technicum e pousio nos atributos físicos de solo cultivado com feijão e milho orgânicos, sob semeadura direta (SD e preparo convencional (PC. O trabalho foi conduzido em Santo Antônio de Goiás-GO, em Latossolo Vermelho distrófico, no delineamento de blocos ao acaso, com quatro repetições. Em novembro de 2003 foram instalados quatro experimentos, dois em SD e dois em PC, sendo um com feijão e outro com milho em cada sistema. Amostragens do solo das parcelas e de uma mata próxima aos experimentos foram realizadas em novembro de 2007, nas camadas de 0,00-0,10 e 0,10-0,20 m, para determinação do teor de matéria orgânica (MO e de atributos físicos do solo. O uso desse solo para a produção agrícola, independentemente do sistema de preparo, resultou em redução no teor de MO e em modificações nos seus atributos físicos, aumentando sua densidade (Ds e resistência à penetração (RP e reduzindo a macroporosidade (Mp, porosidade total (Pt e diâmetro médio ponderado dos agregados (DMP. Entretanto, a Ds e a Mp não atingiram os valores críticos preconizados na literatura como limitantes ao desenvolvimento das culturas. Os sistemas de preparo do solo divergiram quanto ao DMP e à RP. Os atributos físicos do solo foram alterados favoravelmente pela MO. O índice S correlacionou-se com os atributos físicos do solo e com o teor de MO, mostrando-se adequado como indicador da qualidade física do solo.

  15. Rendimento do feijoeiro influenciado por sistemas de manejo em um Latossolo Vermelho de cerrado = Influence of management systems in Oxisol on common bean yield

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    Luis Gustavo Akihiro Sanches Suzuki

    2007-01-01

    Full Text Available Objetivou-se avaliar a influência de sistemas de manejo no rendimento de grãos de feijão em um Latossolo Vermelho de cerrado. A pesquisa iniciou-se em 1997 e a avaliação do rendimento de grãos foi realizada no inverno de 2002. Utilizou-se o delineamento experimental de blocos ao acaso no esquema em faixas com parcelas subsubdivididas. Os tratamentos foram constituídos nas faixas pela combinação de 5 plantasde cobertura (Mucuna aterrima, Pennisetum americanum, Crotalaria juncea, Cajanus cajan e pousio; no outro sentido, as faixas foram subdivididas e constituídas pelo sistema de semeadura direta e convencional (preparo do solo com grade aradora e grade niveladora, as quais, por sua vez, foram subsubdivididas com as culturas de verão milho, soja e algodão. Concluiu-se que as plantas de cobertura, após cinco anos, não influenciaram o rendimento de grãos; a massa de 100 grãos de feijão foi maior na semeadura convencional, porém, orendimento não diferiu entre os preparos; na semeadura convencional, a sucessão com algodão, nos dois primeiros anos agrícolas, proporcionou grãos de feijão mais pesados e maior rendimento; na semeadura direta, a sucessão que incluía algodão ou soja nos doisprimeiros anos agrícolas proporcionou maiores rendimentos ao feijoeiro.This study aimed to evaluate the influence of management on common bean in Oxisol. The research began in 1997 and the yield evaluation was carried out in the winter of 2002. The experimental design was randomized blocks with split split-plot design. The treatments were constituted by the combination of five cover plants, two soil tillages and three crop successions. It was concluded that cover plants, after five years, did not influence the common bean; the 100-grain weight of bean was higher in conventional tillage, but theyield did not show any difference between the tillages; in conventional tillage, cotton crop sucession in the first two years provided larger common

  16. Calidad de las semillas de accesiones de leguminosas colectadas en la región oriental de Cuba (Nota técnica

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    Yolanda González

    Full Text Available Se efectuó una misión de colecta durante el 2006 en la región oriental de Cuba, que comprendió zonas de las provincias de Granma, Guantánamo, Holguín, Bayamo, Las Tunas y Camagüey. Se colectaron semillas de 95 accesiones de leguminosas, de ellas 68 herbáceas y 27 arbóreas. Posteriormente se hizo una prueba de germinación, para conocer su calidad (con corte de cubierta. Para ello se utilizaron placas de Petri sobre arena de río. El número de semillas utilizadas fue variable en cada accesión (según la cantidad colectada y solo se empleó una réplica (sin diseño estadístico. Los conteos de germinación se realizaron según las normas internacionales. Las semillas de mejor calidad correspondieron a las especies arbóreas, aunque presentaron baja germinación las de Cajanus cajan, Cassia siamea, Peltophorum ferrugianum y Leucaena leucocephala, procedentes de la zona de Floro-Pérez en la provincia de Holguín. Todas mostraron 100% de sobrevivencia. La calidad de las semillas dependió del tiempo que estuvieron en el campo después de la etapa de maduración de las legumbres. A través de esta misión de colecta se incrementó el germoplasma de la EEPF «Indio Hatuey» con 64 nuevas accesiones. Se recomienda la continuidad de la colecta de germoplasma en otras zonas del país, con énfasis en las plantas arbóreas, lo que permitirá lograr una mayor diversidad a partir de su uso en los sistemas de producción agropecuaria, así como llevar a cabo las pruebas de calidad correspondientes.

  17. Effects of pigeon pea and plantain starches on the compressional, mechanical, and disintegration properties of paracetamol tablets.

    Science.gov (United States)

    Dare, Kunle; Akin-Ajani, Dorothy O; Odeku, Oluwatoyin A; Itiola, Oludele A; Odusote, Omotunde M

    2006-03-01

    A study has been made of the effects of pigeon pea starch obtained from the plant Cajanus cajan (L) Millisp. (family Fabaceae) and plantain starch obtained from the unripe fruit of Musa paradisiaca L. (family Musaceae) on the compressional, mechanical, and disintegration properties of paracetamol tablets in comparison with official corn starch BP. Analysis of compressional properties was done by using density measurements, and the Heckel and Kawakita equations, whereas the mechanical properties of the tablets were evaluated by using tensile strength (T--a measure of bond strength) and brittle fracture index (BFI--a measure of lamination tendency). The ranking for the mean yield pressure, P(y), for the formulations containing the different starches was generally corn plantain starch while the ranking for P(k), an inverse measure of the amount of plasticity, was pigeon pea plantain starch, which indicated that formulations containing corn starch generally exhibited the fastest onset of plastic deformation, whereas those formulations containing pigeon pea starch exhibited the highest amount of plastic deformation during tableting. The tensile strength of the tablets increased with increase in concentration of the starches while the Brittle Fracture Index decreased. The ranking for T was pigeon pea > plantain > corn starch while the ranking for BFI was corn > plantain > pigeon pea starch. The bonding capacity of the formulations was in general agreement with the tensile strength results. The disintegration time (DT) of the formulation increased with concentration of plantain and corn starches but decreased with concentration of pigeon pea starch. The general ranking of DT values was plantain starch. Notably, formulations containing pigeon pea starch exhibited the highest bond strength and lowest brittleness, suggesting the usefulness of pigeon pea starch in producing strong tablets with minimal lamination tendency. Plantain starch, on the other hand, would be more

  18. Bradyrhizobium spp. Strains in Symbiosis with Pigeon Pea cv. Fava-Larga under Greenhouse and Field Conditions

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    Márcia Rufini

    Full Text Available ABSTRACT: Optimization of symbiosis between nitrogen-fixing bacteria and legumes has been extensively studied, seeking agricultural sustainability. To evaluate the symbiotic efficiency of nitrogen-fixing bacterial strains belonging to the Bradyrhizobium genus with pigeon pea (Cajanus cajan (L. Millsp. cv. Fava-Larga, experiments were conducted in Leonard jars (axenic conditions, pots with soil, and in the field. Ten strains were tested in Leonard jars, and three strains, in addition to BR 29, were selected according to their ability to promote the growth of pigeon pea, for further tests in pots with different soil types (Inceptsol and Oxisol and in the field (Oxisol. Treatments were compared with strains BR 2003 and BR 2801 (approved as inoculants for pigeon pea, with a non-inoculated control with mineral N fertilization, and with another non-inoculated control (absolute control with low mineral N concentration (Leonard jars or without mineral N fertilization (soil. The efficiency of Bradyrhizobium strains in axenic conditions varies among strains, being higher when pigeon pea cv. Fava-Larga establishes symbiosis with the strains UFLA 03-320, UFLA 03-321, UFLA 04-212, BR 2801, and BR 2003. The soil type influences the symbiotic efficiency of Bradyrhizobium-pigeon pea in soil in the greenhouse, mainly in Inceptsol, in which strains UFLA 04-212, BR 2801, and BR 2003 increased N accumulation in the plant. The strain UFLA 03-320 increased shoot dry matter and N accumulation in the shoot equivalent to the mineral N treatment under field conditions. UFLA 03-320, BR 29, UFLA 03-321, and UFLA 04-212 promoted yields similar to those of the reference strain (BR 2801, and of the mineral N treatment with 70 kg ha-1 urea-N. These results confirm that pigeon pea establishes efficient symbiosis, which provides the N required for its growth. All strains, except for BR 2003, show potential for recommendation as inoculants for grain production. The strain UFLA 03

  19. Leguminosas estivais intercalares como fonte de nitrogênio para o milho, no sul do Brasil

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    E. Spagnollo

    2002-06-01

    Full Text Available O uso de leguminosas para cobertura do solo e suprimento de N é uma técnica importante na conservação do solo e redução dos custos em fertilizantes minerais. No sul do Brasil, o enfoque principal da pesquisa tem sido sobre espécies de ciclo hibernal. No presente estudo, avaliou-se o efeito do cultivo intercalar de quatro leguminosas estivais [feijão-de-porco (Canavalia ensiformis , guandu anão (Cajanus cajan, mucuna cinza (Stizolobium niveum e soja preta (Glycine sp.] no fornecimento de N e rendimento de grãos do milho, em três doses de N mineral (0, 60 e 120 kg ha-1, em comparação a um tratamento-testemunha, sem leguminosa. Dois experimentos foram realizados, em sistema de preparo mínimo-PM (1993 a 1997, com abertura de sulcos para semeadura do milho feita com arado de tração animal, e em sistema de preparo convencional-PC (1994 a 1997, com aração e duas gradagens na primavera-verão, num Latossolo Vermelho distroférrico, em Chapecó (SC. Na média das safras, a produção de matéria seca das leguminosas estivais variou de 1,26 a 5,48 Mg ha-1, e o N na fitomassa da parte aérea, de 31 a 132 kg ha-1. Em comparação ao tratamento-testemunha, sem N mineral, as leguminosas aumentaram o rendimento do milho em 17 a 93 % (423 a 2.256 kg ha-1, no PC, e em 12 a 43 % (281 a 1.030 kg ha-1, no PM, sendo estes incrementos equivalentes à aplicação de 12 a > 120 kg ha-1 de N mineral no PC e de 50 a 86 kg ha-1 de N mineral no PM. Portanto, o cultivo intercalar de leguminosas estivais, além de proteger o solo, representa uma técnica promissora no forneci-mento de N e aumento de rendimento do milho, recomendada principalmente para pequenas propriedades rurais, onde a colheita do milho é manual.

  20. Produção e estado nutricional do milho em cultivo intercalar com adubos verdes

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    R. Heinrichs

    2002-03-01

    Full Text Available A utilização da adubação verde para estabelecer a diversidade e o equilíbrio do sistema de produção é um dos paradigmas da agricultura moderna. Com o objetivo de avaliar a produção e o estado nutricional da cultura de milho sob cultivo intercalar com adubos verdes, foi realizado, entre 1995 e 1997, um experimento em campo, em Piracicaba (SP, em um Litossolo Vermelho eutrófico. O milho foi semeado no espaçamento de 0,90m nas entrelinhas, perfazendo aproximadamente 50.000plantas por hectare. Os tratamentos constaram de uma testemunha, sem cultivo intercalar, e quatro espécies de adubos verdes: mucuna anã (Mucuna deeringiana (Bort. Merr, guandu anão (Cajanus cajan (L. Millsp, crotalária (Crotalaria spectabilis Roth e feijão-de-porco (Canavalia ensiformis (L. DC., semeados sem adubação, no meio da entrelinha, distante 45cm da linha do milho, em duas épocas: simultânea à semeadura do milho e 30dias após. O solo, no primeiro ano, foi preparado sob sistema convencional e, no segundo, cultivaram-se as culturas sob semeadura direta. O delineamento experimental foi de blocos ao acaso com parcelas subdivididas e quatro repetições. O estado nutricional e a produção de grãos do milho apresentaram melhores resultados no cultivo consorciado com feijão-de-porco. Os efeitos positivos desta espécie na produtividade de grãos de milho foram mais acentuados no segundo ano de adoção do cultivo consorciado, quando o sistema de manejo do solo foi semeadura direta. A semeadura dos adubos verdes simultânea ao milho foi o manejo mais recomendável, por não prejudicar o desenvolvimento do milho e reduzir a operação pós-plantio.

  1. Atributos bioquímicos e químicos do solo rizosférico e não rizosférico de culturas em rotação no sistema de semeadura direta

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    Meire Aparecida Silvestrini Cordeiro

    2012-12-01

    Full Text Available Sistemas autossustentáveis favorecem as populações microbianas devido à conservação e ao aumento da matéria orgânica no solo. Além disso, as plantas que fazem parte desses sistemas promovem o efeito rizosférico, por meio da zona de influência das raízes, que resulta no aumento da atividade e na modificação da população microbiana. O objetivo deste trabalho foi avaliar o efeito da rotação de culturas de inverno sobre sequências de verão, em sistema de semeadura direta, nos atributos bioquímicos (amilase, urease, celulase e protease e químicos (carbono orgânico total - COT, carboidratos totais e proteínas totais em solo rizosférico (SR e não rizosférico (SNR. Este estudo foi constituído de três culturas de inverno: milho (Zea mays L., girassol (Helianthus anuus L. e guandu (Cajanus cajan (L. Millsp, que estavam em rotação sobre três sequências de verão: soja/soja (Glycine max L., milho/milho e soja/milho, e duas posições no solo: solo aderido às raízes das plantas (SR e solo da entrelinha de plantio (SNR. As atividades da amilase, celulase, protease e urease no SR foram 16, 85, 62 e 100 % maiores do que no SNR; para COT e proteínas totais a diferença foi de 21 %. Das culturas de inverno, o milho foi a que mais estimulou as atividades das enzimas amilase, celulase, urease e protease no SR, bem como a atividade das enzimas amilase, urease e protease no SNR. De modo geral, os teores de proteínas totais não foram influenciados pelas culturas de inverno e pelas sequências de verão; os carboidratos totais foram influenciados pelas culturas de inverno milho e girassol. Para o COT houve influência apenas da sequência de verão milho/milho. Os atributos bioquímicos e químicos avaliados neste estudo podem ser utilizados como indicadores das alterações no solo promovidas pelas culturas de inverno e pelas sequências de verão.

  2. Coberturas vegetais, doses de nitrogênio e inoculação de sementes com Azospirillum brasilense em arroz de terras altas no sistema plantio direto

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    Douglas de Castilho Gitti

    2012-01-01

    Full Text Available O arroz é importante fonte de energia e proteínas para a população mundial, principalmente na Ásia e Oceania. No Brasil, juntamente com o feijão, constitui a base da alimentação. Tecnologias sustentáveis que reduzam custos da produção e aumentem a produtividade do arroz podem garantir seu suprimento em períodos de alta demanda. O objetivo deste trabalho foi avaliar diferentes coberturas vegetais antecessoras (milheto [Pennisetum americanum], crotalária [Crotalaria juncea], guandu [Cajanus cajan], braquiária [Brachiaria ruziziensis], milheto + crotalária e milheto + guandu, doses de nitrogênio (N em cobertura (0, 40, 80 e 120 kg ha-1 e o efeito da inoculação de sementes com Azospirillum brasilense no arroz de terras altas em sistema plantio direto no desenvolvimento e na produtividade. Utilizou-se o delineamento experimental em blocos casualizados em esquema fatorial 6x4x2 com quatro repetições. O estudo foi desenvolvido em um Latossolo Vermelho, em Selvíria, Estado do Mato Grosso do Sul, Brasil, em 2011/2012. O cultivo do guandu antecedendo o arroz proporcionou maior produtividade do arroz somente em comparação a B. ruziziensi. A produtividade do arroz em função das doses de N em cobertura se ajustou a uma função quadrática. Não houve influência da inoculação de sementes com A. brasilense sobre a produtividade do arroz, porém houve interação entre a inoculação e as coberturas vegetais sobre o teor de N foliar, número de panículas por m², matéria seca de plantas de arroz e a massa de cem grãos.

  3. Avaliação de coberturas mortas em cultura de alface sob manejo orgânico Evaluation of mulches on organically grown lettuce

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    Fabio F de Oliveira

    2008-06-01

    Full Text Available A cobertura morta do solo com leguminosas e gramíneas é uma prática cultural que traz benefícios aos sistemas de produção. Foi conduzido um experimento no município de Seropédica, estado do Rio de Janeiro, com o objetivo de avaliar o efeito de diferentes tipos de cobertura morta sobre a reinfestação de ervas espontâneas e sobre o desempenho agronômico de alface, em cultivo orgânico. O delineamento experimental adotado foi blocos casualizados com quatro repetições e parcelas de oito plantas na área útil. Os tratamentos foram: bagaço de cana-de-açúcar (Saccharum sp., bambu (Bambuza sp., capim Cameroon (Penisetum purpureum, crotalária (Crotalaria juncea, eritrina (Erythrina poeppigiana, gliricídia (Gliricidia sepium, guandu (Cajanus cajan, mucuna cinza (Mucuna pruriens e controle (sem cobertura dos canteiros. Estimou-se a decomposição in situ e a liberação de nitrogênio de cada cobertura morta. Foram avaliados dois ciclos consecutivos de alface, cultivar Regina, na mesma área, visando a comparar o efeito residual das coberturas mortas. Observou-se maior acúmulo de N nos resíduos vegetais das leguminosas (máximo de 1.010 kg ha-1 com mucuna-cinza. Em relação à decomposição da cobertura morta, avaliada na colheita do primeiro ciclo de alface (35 dias após o transplante, as leguminosas apresentaram percentuais inferiores aos das gramíneas de massa seca e N remanescentes. A reinfestação dos canteiros pelas ervas espontâneas não diferiu significativamente entre coberturas mortas, variando entre 31 e 58 plantas m-2, mas a redução da densidade populacional da vegetação reinfestante chegou a 83% em comparação ao tratamento controle. Em ambos os ciclos de cultivo da alface, a massa fresca (de 315,8 a 366,0 e de 202,9 a 225,0 g planta-1, respectivamente nos primeiro e segundo ciclos, o diâmetro da cabeça (de 30,8 a 31,7 e de 25,5 a 28,5 cm e o teor de N (de 32,3 a 38,8 e de 28,0 a 30,3 g kg-1 foram superiores

  4. Frações oxidáveis do carbono orgânico em argissolo vermelho-amarelo sob sistema de aleias Oxidizable organic carbon fractions of an ultisol under an alley cropping system

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    Arcângelo Loss

    2009-08-01

    Full Text Available O aporte de matéria orgânica ao solo via leguminosas em sistemas de aleias pode ser uma alternativa para o uso sustentável do trópico úmido. O objetivo deste trabalho foi comparar o aporte de matéria orgânica facilmente oxidável proveniente da combinação de resíduos de diferentes leguminosas utilizadas em sistemas de condução de culturas em aleias sob Argissolo Vermelho-Amarelo. Foram avaliadas duas espécies de leguminosas de alta qualidade de resíduos - leucena (Leucaena leucocephala e guandu (Cajanus cajan, e duas espécies de baixa qualidade de resíduos - sombreiro (Clitoria fairchildiana e acácia (Acacia mangium, combinadas entre si, nos seguintes tratamentos: sombreiro + guandu; leucena + guandu; acácia + guandu; sombreiro + leucena; leucena + acácia e testemunha sem leguminosa. As amostras de solo foram coletadas nas profundidades de 0-5 e 5-10 cm, nas entrelinhas. Foi quantificado e fracionado o C orgânico total (COT, estratificado em quatro frações (F1, F2, F3 e F4 com graus decrescentes de oxidação. As áreas com sombreiro/leucena, acácia/leucena e acácia/guandu apresentaram os maiores teores de COT (0-5 cm, e a área testemunha, os menores (5-10 cm. A maior proporção do COT estava nas frações F1+F2; a área testemunha teve os menores teores de C nessas frações. O tratamento acácia/guandu apresentou maiores teores de C na fração F1 nas duas profundidades. O tratamento leucena/guandu apresentou as maiores proporções do COT nas frações F3 + F4. Os resultados indicam que, no cultivo em sistema de aleias, a combinação de leguminosas de alta e baixa qualidade de resíduos (acácia/guandu favorece o aumento de matéria orgânica facilmente decomponível, enquanto o uso de leguminosas de alta qualidade de resíduos favorece maiores proporções de C somente nas frações mais resistentes (F3 + F4.The input of organic matter to the soil by legumes in alley cropping systems may be an alternative for a

  5. Atributos bioquímicos e químicos do solo rizosférico e não rizosférico de culturas em rotação no sistema de semeadura direta Biochemical and chemical attributes of rhizospheric and non-rhizospheric soil in no till crop rotation system

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    Meire Aparecida Silvestrini Cordeiro

    2012-12-01

    Full Text Available Sistemas autossustentáveis favorecem as populações microbianas devido à conservação e ao aumento da matéria orgânica no solo. Além disso, as plantas que fazem parte desses sistemas promovem o efeito rizosférico, por meio da zona de influência das raízes, que resulta no aumento da atividade e na modificação da população microbiana. O objetivo deste trabalho foi avaliar o efeito da rotação de culturas de inverno sobre sequências de verão, em sistema de semeadura direta, nos atributos bioquímicos (amilase, urease, celulase e protease e químicos (carbono orgânico total - COT, carboidratos totais e proteínas totais em solo rizosférico (SR e não rizosférico (SNR. Este estudo foi constituído de três culturas de inverno: milho (Zea mays L., girassol (Helianthus anuus L. e guandu (Cajanus cajan (L. Millsp, que estavam em rotação sobre três sequências de verão: soja/soja (Glycine max L., milho/milho e soja/milho, e duas posições no solo: solo aderido às raízes das plantas (SR e solo da entrelinha de plantio (SNR. As atividades da amilase, celulase, protease e urease no SR foram 16, 85, 62 e 100 % maiores do que no SNR; para COT e proteínas totais a diferença foi de 21 %. Das culturas de inverno, o milho foi a que mais estimulou as atividades das enzimas amilase, celulase, urease e protease no SR, bem como a atividade das enzimas amilase, urease e protease no SNR. De modo geral, os teores de proteínas totais não foram influenciados pelas culturas de inverno e pelas sequências de verão; os carboidratos totais foram influenciados pelas culturas de inverno milho e girassol. Para o COT houve influência apenas da sequência de verão milho/milho. Os atributos bioquímicos e químicos avaliados neste estudo podem ser utilizados como indicadores das alterações no solo promovidas pelas culturas de inverno e pelas sequências de verão.Self-sustaining systems encourage microbial populations due to the conservation and

  6. Comparison of carbon and nitrogen determination methods for samples of a Paleudult subjected to no-till cropping systems Comparação de métodos de determinação de carbono e nitrogênio em amostras de Argissolo sob plantio direto

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    Jeferson Dieckow

    2007-10-01

    Full Text Available Organic carbon (C concentration evaluated by the Walkley-Black method, and total nitrogen(Nconcentration determined by Kjeldahl method, were compared with corresponding results of C and N concentrations obtained through the dry combustion method (Elementar Vario EL analyzer, using samples of ten soil layers down to the depth of 107.5 cm of a kaolinitic Typic Paleudult (220-418 g clay kg-1 along the profile subjected to no-till cropping systems [fallow bare soil, pigeon pea (Cajanus cajan L. Millsp. plus maize, and lablab (Lablab purpureum L. Sweet plus maize]. A close correlation (R² > 0.96 was observed between the C results of the Walkley-Black and dry combustion methods, but a correction was suggested to be applied to C results of Walkley-Black (C = 1.05C W.Black + 0.47. A close correlation (R² > 0.96 was also found between results of Kjeldahl-N and dry combustion-N, but no correction was necessary to be applied. The relationships between results of Walkley-Black and dry combustion and between results of Kjeldahl and dry combustion did not change among soil samples from different management systems, in disagreement to findings of a previous study where results of analytical C recovery were influenced by samples from different managements, supposedly because changes in lability or recalcitrance of organic matter might have affected the wet combustion reactions. A poor correlation (R² = 0.42 was found between the C:N ratios based on Walkley-Black and Kjeldahl analysis and the C:N ratios based on dry combustion analysis. The methods for C and N determination did not affect the interpretation of the effects of management systems on soil C and N stocks.Resultados de concentração de carbono (C, obtidos pelo método Walkley-Black, e resultados de concentração de nitrogênio (N total, determinados pelo método Kjeldahl, foram comparados com os correspondentes resultados de C e N obtidos pelo método de combustão seca (analisador Elementar

  7. Crescimento aéreo e radicular da soja e de plantas de cobertura em camadas compactadas de solo Shoot and root growth of soybean and cover crops as affected by soil compaction

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    José Salvador Simoneti Foloni

    2006-02-01

    Full Text Available O cultivo de plantas de cobertura com sistema radicular vigoroso em rotação de culturas pode melhorar a qualidade física de solos compactados. O objetivo deste trabalho foi avaliar o crescimento aéreo e radicular da soja e de cinco espécies utilizadas como plantas de cobertura de verão (guandu, guandu anão, mucuna preta, labe labe e crotalária juncea, em solo submetido a três níveis de compactação em subsuperfíce. Utilizou-se um Nitossolo Vermelho argiloso em vasos montados com anéis de PVC sobrepostos, com diâmetro interno de 20 cm e altura de 50 cm. A camada compactada localizada de 20-30 cm de profundidade foi caracterizada pelas densidades do solo de 1,13, 1,34 e 1,56 g cm-3 e 0,26, 0,66 e 1,98 MPa de impedância mecânica, respectivamente. Aos 60 dias da emergência das plantas, determinaram-se as massas da matéria seca da parte aérea e das raízes, a densidade do comprimento radicular e o diâmetro médio radicular. Apesar de alterar a distribuição do sistema radicular ao longo do perfil do solo, o impedimento físico em subsuperfície não diminuiu a produção total de raízes da soja e dos adubos verdes, com exceção da crotalária juncea. A mucuna preta foi a leguminosa mais tolerante e a soja a mais sensível à compactação do solo. O sistema radicular da crotalária juncea apresentou potencial de formação de "bioporos" compatível ao da mucuna preta, apesar de ter sido relativamente mais sensível à compactação do solo. O diâmetro médio das raízes da soja e da crotalária juncea apresentou correlação significativa com o crescimento radicular dentro da camada de solo compactado.The cultivation of cover crops with vigorous root systems in crop rotation can improve the physical quality of compacted soils. The objective of this study was to evaluate shoot and root growth of soybean (Glycine max and five species used as summer cover crops (Cajanus cajan, Cajanus cajan var. small, Mucuna aterrima, Dolichos

  8. In situ caecal degradation of roughages in horses Degradação cecal in situ de alimentos volumosos em equinos

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    Vinícius Pimentel Silva

    2010-02-01

    Full Text Available The present study was carried out to evaluate the in situ degradation of dry matter (DM, neutral detergent fiber (NDF and crude protein (CP in roughages by the in situ caecal digestion technique in horses. The roughages evaluated were: Lucerne hay (Medicago sativa, peanut (Arachis pintoi cv. Amarillo, desmodio(Desmodium ovalifolium, stylo (Stylosanthes guianensis cv. Mineirão, pigeon pea (Cajanus cajan, lime-yellow pea (Macrotyloma axillare and coastcross hay (Cynodon dactylon cv. coastcross. The assay was conducted in a complete randomized design with seven roughages and three replications. One mare with a cannula fitted in the caecum was used, fed diet consisting of coastcross hay (80% and concentrate (20% at 2.0% BW, four times a day. Nylon 6.5 × 20 cm bags were used with 45 μ/pore, containing 5.2 g DM/bag, inserting 3 or 4 bags in the caecum at the times of 2, 4, 6, 8, 12, 24 and 48 hours incubation. The caecum in situ degradability parameters of nutrients were obtained by Ørskov model. The DM degradability parameters of all the roughages were significant. There was no fit to the model for pigeon pea for CP and NDF and desmodio. Peanut, stylo and lime-yellow pea presented larger potentially degradable DM with values of 53, 46.5 and 40%, respectively, and higher values for the soluble fraction of 20, 21, 28.6%, with high degradability rates of 10.36, 20.26 and 14.8% h-1. Higher NDF degradation rates were observed in these foodstuffs with values of 9.1 and 11.3, 11.2% h-1, high potentially degradable fraction with values of 55, 51.8 and 47.2%, and greater CP degradation at 48 hours with values of 87, 95, and 94.8%. Peanut, stylo and lime-yellow pea presented potential for use in horses diets.Objetivou-se avaliar a degradação da matéria seca (MS, fibra em detergente neutro (FDN e proteína bruta de alimentos volumosos pela técnica da digestão cecal in situ em equinos. Avaliaram-se as forrageiras: feno de alfafa (Medicago sativa

  9. Legumes and forage species sole or intercropped with corn in soybean-corn succession in midwestern Brazil Espécies leguminosas e forrageiras, solteiras ou consorciadas com milho, na sucessão soja-milho no centro-oeste do Brasil

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    Gessí Ceccon

    2013-02-01

    Full Text Available The feasibility of no-tillage in the Cerrado (Savanna-like vegetation of Brazil depends on the production of sufficient above-ground crop residue, which can be increased by corn-forage intercropping. This study evaluated how above-ground crop residue production and yields of soybean and late-season corn in a soybean-corn rotation were influenced by the following crops in the year before soybean: corn (Zea mays L. intercropped with Brachiaria (Urochloa brizantha cv. Marandu, B. decumbens cv. Basilisk, B. ruziziensis, cv. comum., Panicummaximum cv. Tanzânia, sunn hemp (Crotalaria juncea L., pigeon pea [Cajanus cajan (L. Millsp]; sole corn, forage sorghum [Sorghum bicolor (L. Moench (cv. Santa Elisa], and ruzi grass. In March 2005, corn and forage species were planted in alternate rows spaced 0.90 m apart, and sole forage species were planted in rows spaced 0.45 m apart. In October 2005, the forages were killed with glyphosate and soybean was planted. After the soybean harvest in March 2006, sole late-season corn was planted in the entire experimental area. Corn grain and stover yields were unaffected by intercropping. Above-ground crop residue was greater when corn was intercropped with Tanzania grass (10.7 Mg ha-1, Marandu (10.1 Mg ha-1, and Ruzi Grass (9.8 Mg ha-1 than when corn was not intercropped (4.0 Mg ha-1. The intercropped treatments increased the percentage of soil surface covered with crop residue. Soybean and corn grain yields were higher after sole ruzi grass and intercropped ruzi grass than after other crops. The intercropping corn with Brachiaria spp. and corn with Panicum spp. increases above-ground crop residue production and maintains nutrients in the soil without reducing late-season corn yield and the viability of no-till in the midwestern region of Brazil.A viabilidade do plantio direto no Cerrado brasileiro depende da produção adequada de palha das culturas, que pode ser aumentada pela consorciação de milho (Zea mays L

  10. Management of crotalaria and pigeon pea for control of yam nematode diseases Manejo da crotalária e do guandu no controle de nematoses do inhame

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    Marlon da Silva Garrido

    2008-09-01

    Full Text Available Management of plant-parasitic nematodes with the use of nematicides has not been recommended for small farmers that grow yam in the Northeastern region of Brazil, due to its high cost and residue toxicity. The use of plants with antagonistic effect to nematodes and green manure which improves soil chemical, physical and biological characteristics can be a viable and low cost alternative to control parasitic nematodes. This work aimed to evaluate the effect of crotalaria (Crotalaria juncea and pigeon pea (Cajanus cajan plants on the control of yam nematodes. Three experiments were carried out. The first was conducted under in vitro conditions to evaluate the nematostatic and nematicide effect of extracts from fresh and dry matter of the above ground parts of crotalaria, pigeon pea, and the combination of both. The second experiment was carried out under greenhouse conditions to evaluate the effect of soil amendment with crotalaria, pigeon pea, and the combination of both in the infectivity of Scutellonema bradys, using tomato plants as the host plant. The third experiment was conducted under field conditions to evaluate the effect of crotalaria, pigeon pea, and the combination of both, cultivated between yam planting rows and incorporated to soil surface, on yam nematodes. The aqueous extract obtained form fresh matter of crotalaria had a nematicide effect of 100% for S. bradys. Extracts from dry matter of both crotalaria and pigeon pea did not have any nematicide effect, but had a nematostatic effect. Incorporation of crotalaria to soil inhibited infectivity of S. bradys in tomato seedlings. These results showed that planting crotalaria alone or in combination with pigeon pea, between the yam planting rows, is an efficient method for controlling S. bradys and Rotylenchulus reniformis associated with yams. Crotalaria can be used for controlling these plant-parasitic nematodes in soil.O manejo de fitomenatóides com o uso de nematicidas não tem

  11. Dicty_cDB: Contig-U05290-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available spinal ganglion cDNA,... 48 0.47 1 ( FI262409 ) CccaBb041-C8JR CccABb Cajanus caja...9763 ) 1095368013982 Global-Ocean-Sampling_GS-34-01-01-1... 34 4.8 2 ( FI242748 ) CccaBb003-O13JR CccABb Cajanus caja

  12. Soil and Water Conservation Function of Composite Shrub Grasslands on the Hot Arid Valley in Yunnan Province%干热河谷区灌草草地的水土保持效应

    Institute of Scientific and Technical Information of China (English)

    毕玉芬; 车伟光; 许岳飞

    2009-01-01

    为了恢复干热河谷退化山区草地的生态环境.提高草地的水土保持能力,于2004~2007年在金沙江干热河谷区的云南省永胜县设置样点.在退化严重的草地上建植了7个组合模式的灌草复合型草地,以凋落物量、最大持水量、有效截流量、草地盖度、草地土壤崩解时间为指标.对建植3年的灌草草地的水保效果进行研究.结果表明."灌+草+藤"最适宜金沙江干热河谷区的植被重建.其中"木豆+非洲狗尾草+高羊茅+大翼豆"建植模式显著地提高草地植被盖度和凋落物重量.凋落物的持水能力与凋落物重量呈显著的正相关;土壤根量与土体崩塌时间呈显著的正相关.灌草草地植被盖度、凋落物量和根系重量的显著提高,有效地提高草地的持水量和有效截流量,推迟草地土壤崩解时间,增加了土体的稳定性,对防止水土流失和泥石流的形成具有重要作用.%In order to recover ecological environment and enhance soil and water conservation capacity of degraded mountainous grasslands in the hot arid valley,7 combined patterns for shrub-grass communities were established on the degraded mountains in the Jinsha River hot-arid valley at Yongsheng County of Yunnan Province from 2004 to 2007. In 2007,litter weight,the largest water-holding capacity,effective cut-off flow,grass cover and grassland soil disintegration time of grassland in this hot arid valley were analyzed. The results showed that "shrub+grass+vine" was the most favorable pattern for vegetation recovery in the area of Jinsha River hot-arid valley. Among them,the combination of "Cajanus cajan + Setaria sphacelata + Festuca arundinacea + Macroptilium atropurpureum" significantly improved the grassland vegetation coverage and litter weight. The water retention capacity was significantly correlated with weight of the litter. Soil root biomass was significantly positively correlated with the soil disintegration time

  13. Behavioral response of small green leafhopper to volatiles from different green manures%假眼小绿叶蝉对不同绿肥挥发性物质的行为反应

    Institute of Scientific and Technical Information of China (English)

    谷明; 林乃铨

    2011-01-01

    采用Y型嗅觉仪测定和比较了茶树重要害虫--假眼小绿叶蝉成虫对14种绿肥枝叶挥发性物质的行为反应.结果表明:在以空气为对照的条件下,罗顿豆、猪屎豆、铺地木蓝、杂三叶、黄花槐、伞房决明、圆叶决明等7种绿肥的挥发性物质对假眼小绿叶蝉具有明显的引诱作用;紫穗槐、黄花决明、小冠花、木豆、紫花苜蓿、白三叶等6种绿肥的引诱作用不显著;多花木兰对假眼小绿叶蝉成虫表现出显著拒避作用.进一步以茶梢为对照,测定了上述7种对叶蝉有明显引诱作用的绿肥的引诱活性差异,获知铺地木蓝、杂三叶、猪屎豆等3种绿肥对假眼小绿叶蝉的引诱作用都显著低于茶梢;罗顿豆对假眼小绿叶蝉的引诱活性极显著低于茶梢;只有圆叶决明对叶蝉的引诱活性明显高于茶梢,且差异达到极显著水平.%Small green leaihopper (SGL) Empoasca vitis (Cothe) is one of the major tea insect pests. In this paper, the behavioral response of SGL to volatiles from the shoots and leaves of 14 species of green manures were investigated by using a Y-tube olfactometer in laboratory. The preliminary results as contrasted with air condition showed that volatiles from Crotalaria pallida Alt. , Indigofera spicata Forssk, Trifolium hybridum Linn., Cassiu surattensis Burm, Lotononis bainesii Baker, Cassia corgmbosa Lam. ,Chamaecrista rotundifolia ( Pers. ) Greene possessed obvious attractive activity to SGL. And volatiles from Amorphafruticosa Linn. ,Cassia alatta Lirn. , Coronilla varia Linn. , Cajanus cajan Linn. ; Medicago sativa Limn. , Trifolium repens Linn. didn't show attraction to SGL, while volatiles from Magnolia multiflora Wang et Min had a repellent effect on SGL. Further oomparision between green manures and tea shoots indicated that the behavioral response of SGL to volatiles from Indigofera spicata, Trifolium hybridum, Crotalaria pallid were significantly weaker than that from

  14. Influência da calagem no rendimento de matéria seca de plantas de cobertura e adubação verde, em casa de vegetação

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    P. R. Ernani

    2001-12-01

    Full Text Available A recuperação de solos degradados é lenta e onerosa. Plantas de cobertura de solo e adubação verde têm sido amplamente utilizadas nesse processo de recuperação, pois promovem alta produção de fitomassa com baixo custo, especialmente se forem tolerantes à acidez. O presente trabalho objetivou avaliar o efeito da calagem no rendimento de matéria seca de dezesseis dessas espécies, em casa de vegetação. Oito espécies de inverno (Lollium multiflorum, Avena strigosa, Lathyrus sativus, Lupinus angustifolius, Pisum sativum, Secale cereale, Vicia sativa e Vicia villosa e oito de verão (Stizolobium niveum, Stizolobium aterrinum, Stizolobium deeringianum, Crotalaria juncea, Crotalaria retusa, Crotalaria spectabilis, Cajanus cajan e Canavalia ensiformes foram cultivadas em amostras da camada arável de dois solos ácidos catarinenses (Latossoso Bruno e Cambissolo Húmico, em Lages (SC, em 1997. Os tratamentos consistiram da aplicação de doses de calcário equivalentes a 0, 0,25, 0,50, 1,00 e 1,50 vezes a quantidade recomendada pelo método SMP para elevar o pH dos solos a 6,0. Os valores de Ca e Mg trocáveis aumentaram linearmente com a calagem e na mesma magnitude, ao redor de 3,0 mmol c kg-1 para cada incremento de 0,1 unidade de pH. O efeito da calagem no rendimento de matéria seca variou com o solo e, principalmente, com a espécie. Dez das 16 espécies não apresentaram aumento no rendimento de matéria seca com o aumento do pH, em pelo menos um dos solos, e somente cinco espécies responderam à calagem de forma semelhante nos dois solos. O pH no qual as espécies tiveram a produção máxima de matéria seca foi igual ou inferior a 5,5 em qualquer dos solos e foi normalmente menor no Cambissolo do que no Latossolo, provavelmente pelo efeito benéfico da matéria orgânica em minimizar a toxidez do Al. Essas espécies podem, portanto, ser cultivadas com sucesso como melhoradoras das propriedades físicas, químicas e biol

  15. Estabilidade de agregados de um latossolo vermelho degradado em recuperação com adubos verdes, calcário e gesso

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    Carolina dos Santos Batista Bonini

    2011-08-01

    Full Text Available A utilização incorreta dos solos e as grandes construções no meio rural vêm causando alterações no ambiente edafoclimático, o que torna os solos menos produtivos, aumentando assim as áreas degradadas. Tendo em vista esse problema, o presente trabalho teve como objetivo avaliar a recuperação de um Latossolo Vermelho que se encontra há 17 anos com o uso de adubos verdes, calcário, gesso e braquiária. Foi utilizada a qualidade da estrutura como indicadora da recuperação do solo. O trabalho foi desenvolvido em delineamento experimental inteiramente casualizado, com nove tratamentos e quatro repetições. Os tratamentos foram: testemunha (solo mobilizado até 1999, sendo depois implantada Brachiaria decumbens; mucuna-preta (Stizolobium aterrimum Piper & Tracy até 1999, substituída por B. decumbens; guandu (Cajanus cajan (L. Millsp até 1994, substituído por feijão-de-porco (Canavalia ensiformis (L. e, a partir de 1999, por B. decumbens; calcário+mucuna-preta até 1999, substituído por B. decumbens; calcário+guandu até 1994, substituído por feijão-de-porco e, a partir de 1999, por B. decumbens; calcário+gesso+mucuna-preta até 1999, substituído por B. decumbens; calcário+gesso+guandu até 1994, substituído por feijão-de-porco e, partir de 1999, por B. decumbens; e duas testemunhas: solo exposto (sem técnica de recuperação e vegetação nativa de Cerrado. Foi avaliada a estabilidade de agregados em água e o conteúdo de matéria orgânica nas camadas de 0-10, 10-20 e 20-40 cm. O solo vem sendo recuperado, e a qualidade da estrutura, comparada à condição natural de Cerrado, na camada de 0-10 cm foi mais alterada positivamente; os tratamentos que receberam calcário e gesso foram os melhores, e o teor de matéria orgânica nessa camada foi superior ao das demais. Abaixo da camada superficial do solo, os tratamentos estão agindo de forma semelhante. O solo exposto, em todas as camadas, apresentou-se com má qualidade

  16. Tempo de Captura de Organismos da Mesofauna do Solo e seus Reflexos na Interpretação de Índices da Comunidade Edáfica

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    Luiz Rodrigues Freire

    2015-10-01

    Full Text Available RESUMO A mesofauna edáfica compreende organismos de dimensões entre 100 μm e 2 mm, sendo importante avaliar os métodos utilizados para sua caracterização. Os objetivos deste trabalho foram definir o período de tempo de permanência do material de solo no extrator Berlese-Tüllgren para a captura dos organismos da mesofauna e determinar o tempo mínimo de extração para a correta utilização dos índices de Shannon, Simpson, Pielou e Margalef, empregados na avaliação da diversidade, dominância e equabilidade das comunidades da mesofauna. O estudo foi conduzido no Departamento de Solos do Instituto de Agronomia da Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, em terreno altamente antropizado. Foram instaladas parcelas experimentais com os seguintes tratamentos: feijão-de-porco (Canavalia ensiformis, mucuna-preta (Mucuna aterrima, feijão-bravo-do-Ceará (Canavalia brasiliensis, guandu (Cajanus cajan e vegetação espontânea, em delineamento de quadrado latino. Em cada uma das unidades experimentais, foram coletadas amostras formadas por serapilheira e material de solo, nos primeiros 5 cm da camada superficial do solo realizada um dia antes do plantio das leguminosas (outubro e, posteriormente, por ocasião do corte da parte aérea das plantas (março. As amostras foram submetidas a uma bateria de extratores do tipo Berlese-Tüllgren, por um período de 15 dias, realizando-se a contagem diária dos organismos. A partir da contagem e identificação em nível de grupo (Ordem/Família dos organismos capturados, foram determinados os índices de Margalef, Simpson, Shannon e Pielou, bem como verificou-se a correlação por ordem de Kendall entre os valores obtidos dia a dia até o 8º dia de extração, usando-se o índice de Shannon como referência. Concluiu-se que é possível a redução do tempo de permanência das amostras de material de solo nos funis Berlese-Tüllgren, não sendo recomendável a ado

  17. Teores de nitrogênio no solo e nas frações da matéria orgânica sob diferentes sistemas de produção de milho

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    W. J. O. Souza

    2000-12-01

    Full Text Available Com o objetivo de avaliar o efeito de sistemas de produção sobre a dinâmica do N no solo e nas frações da matéria orgânica, realizou-se, por seis anos, em Jaboticabal (SP, um experimento constituído de: semeadura convencional de milho com pousio no inverno (C-Mi-P, plantio direto de milho e pousio no inverno (D-Mi-P, plantio convencional de milho em rotação com soja e pousio no inverno (C-Mi-P-So, plantio direto de milho em rotação com soja e pousio no inverno (D-Mi-P-So e plantio direto de milho com uso de Mucuna aterrina (mucuna-preta, Cajanus cajan (feijão guandu e Crotalaria juncea no inverno (D-Mi-Mu, D-Mi-Gu e D-Mi-Cr, em delineamento de blocos ao acaso e parcelas subdivididas. Após 60 dias da emergência das plântulas, coletaram-se amostras de solo (0-0,05, 0,05-0,10 e 0,10-0,20 m e planta. Nas amostras de terra, avaliaram-se os teores de N total, N-NH4+, N-NO3-, N biomassa microbiana (N-B, N potencialmente mineralizável (NPM, N total nas frações: matérias húmicas (MH, solúvel em água (FSA, ácido fúlvico (AF, ácido húmico (AH e humina (HN com dados expressos em base de TFSE. Nas folhas, determinaram-se os teores de N total, e nas plantas de adubo verde (semeadas após a colheita do milho, mediram-se a produção de matéria seca (MS. Observaram-se valores maiores de NPM na camada de 0-0,05 m e nos tratamentos com plantio direto, envolvendo ou não rotação de culturas ou adubação verde, com a FSA se comportando de modo semelhante. O sistema de cultivo convencional, envolvendo ou não rotação de culturas, proporcionou maiores valores de N mineral na camada de 0,05-0,10 m, em razão da presença de maiores valores de N-nítrico. O cultivo convencional acelerou o processo de mineralização do N, enquanto a maior adição de matéria orgânica pela cultura de inverno no sistema de plantio direto promoveu incremento da fração potencialmente mineralizável do N no solo.

  18. Comportamento de atributos relacionados com a forma da estrutura de Latossolo Vermelho sob sistemas de preparo e plantas de cobertura Structural attributes of a clayey Hapludox cultivated under distinct tillage methods and cover crops

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    Jeferson Argenton

    2005-06-01

    Full Text Available Os sistemas de preparo de solo e de culturas têm influência significativa na estrutura do solo e nos fluxos de água e ar. Este trabalho objetivou avaliar os efeitos do manejo do solo sobre as propriedades relacionadas com estrutura de um Latossolo Vermelho cultivado com milho intercalado com plantas de cobertura de verão. Dois experimentos foram realizados em Chapecó (SC, em preparo reduzido (PR, de 1993 a 1998, e preparo convencional (PC, de 1994 a 1998, nos quais foram avaliados três sistemas de cultura: milho + guandu anão, milho + mucuna cinza e milho isolado. Adjacente aos experimentos, foi avaliada uma mata nativa. Em comparação com a mata nativa, os sistemas de preparo modificaram a estrutura do solo, com aumento da densidade e da resistência do solo à penetração e redução da macroporosidade e da porosidade total. Após cinco anos de uso, o PR com milho isolado não recuperou as propriedades relacionadas com a forma da estrutura; entretanto, no sistema milho intercalado com mucuna cinza, notou-se aumento da macroporosidade, porosidade total e condutividade hidráulica saturada, bem como a redução da densidade do solo. Estas melhorias, advindas do uso de plantas de cobertura, não foram observadas no sistema de preparo convencional. O uso de culturas intercalares foi adequado para melhorar a qualidade física desse Latossolo Vermelho argiloso, especialmente pelo maior aporte de resíduos de culturas.Soil tillage and crop systems have significant influence on the soil structure, affecting water and air flow. The objective of this study was to evaluate the effects the management has on the soil properties of a Hapludox cultivated with maize intercropped with summer cover crops. Two experiments were carried out in Chapecó, State of Santa Catarina, Brazil under reduced tillage (RT from 1993 to 1998 and conventional tillage (CT from 1994 to 1998. Three cropping systems were evaluated under each tillage system: maize + Cajanus

  19. Fitomassa, teor e acúmulo de micronutrientes do milheto, feijão-deporco e guandu-anão, em cultivo solteiro e consorciado = Micronutrient phytomass, levels and accumulation in millet, jack bean and pigeon pea, as sole crops and in intercropping

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    Cícero Monti Teixeira

    2008-10-01

    pea (Cajanus cajan, dwarf cultivar, as sole crops and intercropping gramineous with leguminous crops, for straw production in a no-tillage system. The experiment was carried out in the experimental field at the Agriculture Department of the Federal University of Lavras (Lavras, Minas Gerais state, Brazil. The experimental was conducted using a randomized block design, with seven treatments for species nutrient levels under different conditions (sole crops orintercropping and five treatments for phytomass production and micronutrient accumulation, with four replications. For phytomass production, only the pigeon pea sole crop displayed loweryield of fresh and dry phytomass, while the others produced on average 12.361 and 2.854 t ha-1 of fresh and dry phytomass yield, respectively. There were significant differences in B, Cu and Znlevels, and in accumulation of all evaluated micronutrients. The leguminous species presented the greatest B levels, with the greatest Cu and Zn levels found in millet. In general, millet plusjack bean intercropping presented the greatest micronutrient accumulation.

  20. Atributos biológicos do solo sob influência da cobertura vegetal e do sistema de manejo Soil biological attributes influenced by cover crops and management systems

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    Mozaniel Batista da Silva

    2007-12-01

    Full Text Available O objetivo deste trabalho foi avaliar os efeitos de culturas de cobertura e dos sistemas plantio direto (PD e convencional (PC sobre indicadores biológicos do solo, cultivado com feijoeiro-comum, no inverno, sob irrigação. O experimento foi conduzido em Santo Antônio de Goiás, GO, em Latossolo Vermelho distrófico textura argilosa. Culturas de cobertura foram implantadas anualmente no verão, desde 2001, sendo utilizadas a braquiária, guandu, milheto, capim-mombaça, sorgo, estilosantes, braquiária consorciada com milho, e mata nativa, como tratamento referência. Em 2005, 60 dias após o corte das culturas de cobertura foi implantada a cultura do feijoeiro, cultivar BRS Valente, sob irrigação, com semeadura realizada em 16/6/2005 e colheita efetuada em 19/9/2005. Coletaram-se amostras de solo, na profundidade de 0-10 cm, em três épocas: novembro de 2004 (pré-plantio das culturas de coberturas, junho (pré-plantio do feijoeiro e julho (florescimento do feijoeiro de 2005. Avaliaram-se a respiração basal, o carbono e o nitrogênio da biomassa microbiana, a razão carbono da biomassa microbiana/carbono orgânico, a razão nitrogênio da biomassa microbiana/nitrogênio total e o quociente metabólico do solo. Esses atributos biológicos do solo são influenciados pelas culturas de cobertura, manejo do solo e épocas de amostragem.The objective of this work was to evaluate the effects of cover crops and direct and conventional tillage systems on soil biological attributes when cultivated with dry bean in winter under sprinkle irrigation. The experiment was conducted in Santo Antônio de Goiás, GO, Brazil, in a clayey Rhodic Haplustox. Cover crops were cultivated annually in the summer since 2001, using Brachiaria brizantha, Cajanus cajan, Pennisetum glaucum, Panicum maximum, sorghum, Stylosanthes guianensis, brachiaria in association with corn, and native vegetation as reference. In 2005, 60 days after cutting the cover crops, BRS

  1. Assessment of Genetic diversity in mutant cowpea lines using ...

    African Journals Online (AJOL)

    FKOLADE

    2016-11-09

    Nov 9, 2016 ... option of NTSYS, a rooted tree was also generated from the .... Dellarporta SF, Wood J, Hicks JB (1983). ... genetic diversity in Pigeon Pea (Cajanus sp). ... diversity in somatic mutants of grape (Vitis vinifera) cultivar Italia.

  2. SUSTENTABILIDADE DE SISTEMAS ORGÂNICOS COM PLANTAS DE COBERTURA NA CULTURA DO ARROZ, POR MEIO DE ALTERAÇÕES FÍSICAS DO SOLO ORGANIC SYSTEMS SUSTAINABILITY USING COVER CROPS IN RICE CULTIVATION THROUGH SOIL PHYSICAL ATTRIBUTES CHANGES

    Directory of Open Access Journals (Sweden)

    Roberta Paula de Jesus

    2010-05-01

    Full Text Available

    O presente trabalho teve como objetivo avaliar o efeito de diferentes plantas de cobertura de solo sobre os atributos físicos de um Latossolo Vermelho distrófico, em sistema de produção orgânico, com preparo convencional. O trabalho foi conduzido, por dois anos, na área experimental da Embrapa Arroz e Feijão, em Santo Antônio de Goiás, GO, em um sistema de sucessão de plantas de cobertura-arroz, no qual as plantas de cobertura eram semeadas no outono/inverno e a cultura subsequente, o arroz, cultivar Aimoré, semeada no verão. Foram utilizadas as seguintes plantas de cobertura: mucuna preta (Mucuna aterrima, crotalária (Crotalaria juncea, guandu-anão (Cajanus cajan, sorgo vassoura (Sorghum technicum e pousio com vegetação espontânea. O delineamento experimental foi de blocos ao acaso, com quatro repetições. Foram coletadas, no segundo ano de experimentação (2005, amostras de solo, nas parcelas e em mata próxima, nas profundidades de 0,00-0,10 m; 0,10-0,20 m; e 0,20-0,30 m, para determinação do teor de matéria orgânica (MO, atributos físicos, índice S e estado de agregação do solo. O manejo das plantas de cobertura de solo foi efetuado por ocasião do florescimento. A biomassa foi incorporada ao perfil por meio de aração. Para a porosidade total, microporosidade e densidade do solo, não foram observadas diferenças significativas entre as profundidades, para as diferentes plantas de cobertura do solo. Para o índice S, observou-se, para todos os tratamentos, na camada 0-10 m de profundidade, valores entre 0,020 e 0,035, que caracterizam solos com qualidade f

  3. 大豆GmNAC73-like基因的克隆和表达及其对GmIFS2基因的影响%Cloning and expression of GmNAC73-like gene from Glycine max and its effect on GmIFS2 gene

    Institute of Scientific and Technical Information of China (English)

    万群; 刘晓庆; 张大勇; 何晓兰; 徐照龙; 宁丽华; 黄益洪; 邵宏波

    2016-01-01

    为研究NAC转录因子对大豆﹝Glycine max ( Linn.) Merr.〕异黄酮合成的影响,根据大豆基因组序列设计引物,从豆荚中克隆获得GmNAC73-like基因,并对该基因序列进行生物信息学分析。结果显示:GmNAC73-like基因包含1个长度981 bp的完整开放阅读框,编码326个氨基酸。 GmNAC73-like蛋白的理论相对分子质量37000,理论等电点pI 6.4,为亲水性蛋白,无信号肽,并被定位在细胞核上,包含核定位信号“PKRRK”。同源性比对结果显示:GmNAC73-like蛋白与野大豆( Glycine soja Sieb. et Zucc.)、蒺藜苜蓿( Medicago truncatula Gaertn.)、可可( Theobroma cacao Linn.)、葡萄( Vitis vinifera Linn.)及拟南芥﹝Arabidopsis thaliana ( Linn.) Heynh.〕的NAC蛋白具有较高的相似性,相似度分别为93%、69%、73%、75%和58%。在NJ系统树上,GmNAC73-like蛋白与野大豆的GsNAC8蛋白和木豆﹝Cajanus cajan ( Linn.) Millsp.〕的CcNAC8蛋白聚在一起,显示出较近的亲缘关系。半定量RT-PCR分析结果显示:在大豆的三叶期、开花期和结荚期,GmNAC73-like基因在根中均不表达,在茎和叶中可不同程度表达且茎中表达量较高;而在开花期或结荚期,该基因在花或豆荚中也可表达,且豆荚中表达量较高。酵母单杂交实验结果显示:GmNAC73-like可与异黄酮生物合成关键酶基因GmIFS2启动子中的CGTG基序结合;在大豆转基因发状根系中过表达GmNAC73-like基因后,除查尔酮异构酶基因的表达量无变化外,其他异黄酮生物合成相关基因的表达量均不同程度提高,其中,肉桂酸-4-羟化酶基因和查尔酮合酶基因的表达量明显提高。此外,在GmNAC73-like基因过表达的大豆转基因发状根系中总异黄酮含量显著降低。综合分析结果表明:GmNAC73-like可能通过与MYB转录因子的互作调控GmIFS2基因的表达,并在大豆异黄酮的生物合成过程中起负调控作用。%In order to study the effect of

  4. Dicty_cDB: Contig-U05649-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 0 ) Strongylocentrotus purpuratus clone R3-63K23, WOR... 36 4.6 4 ( FI264051 ) CccaBb053-H23JR CccABb Cajanus caja...9422 ) PL04016A1A03 cDNA from sexually mature hermaphodi... 34 7.2 2 ( FI233208 ) CccaBb022-K6JF CccABb Cajanus caja

  5. Evaluating sustainable and profitable cropping sequences with cassave and four legume crops: Effects on soil fertility and maize yields in the forest/savannah transitional agro-ecological zone of Ghana

    NARCIS (Netherlands)

    Adjei-Nsiah, S.; Kuyper, T.W.; Leeuwis, C.; Abekoe, M.K.; Giller, K.E.

    2007-01-01

    Rotations are important practices for managing soil fertility on smallholder farms. Six cropping sequences (cassava, pigeonpea, mucuna-maize-mucuna, cowpea-maize-cowpea, maize-maize-maize, and speargrass fallow) were evaluated during 2003-2004 in Wenchi district of Ghana for their effects on the pro

  6. Phosphorus Uptake of Three Tropical Legumes

    African Journals Online (AJOL)

    KH2P04 was also used as starter fertilizer and its effect on utilization of the rock .... The objective of this study was to investigate whether .... Duncan's Multiple Range Test (DMRT) at. P = 0.05. ... the case of pigeonpea and groundnut. In cowpea ...

  7. Contribution of Legume Rotations to the Nitrogen Requirements of a ...

    African Journals Online (AJOL)

    of legumes rotation in meeting the N fertilizer requirements of maize. The experimental site ... This study was carried out on a Rhodic Ferralsol at. Mlingano Agricultural .... different according to Duncan's New Multiple Range Test. *, ** and *** indicate .... and 16 kg N ha-1 in the case of the cowpea, pigeonpea and greengram ...

  8. Ratooning and perennial staple crops in Malawi. A review

    NARCIS (Netherlands)

    Rogé, Paul; Snapp, Sieglinde; Kakwera, Mayamiko Nathaniel; Mungai, Leah; Jambo, Isaac; Peter, Brad

    2016-01-01

    The management of staple crops as perennials is a historic legacy and a present-day strategy in some regions of Sub-Saharan Africa, yet perenniality is rarely an agronomic subject. Farmers in Malawi cut annual crops, such as pigeonpea and sorghum, to extend production for more than one growing se

  9. Download this PDF file

    African Journals Online (AJOL)

    achlevedby the improved fallows ofS. sesban, T. vogelii and C cajan than the natural fallow. .... This study was therefQre cQnducted with the ~im minQr treatments were S. sesban;' t: .. vogelii .... ll?ed to. separate signiftcantly diff~rent m€ans.

  10. Productivity and residual benefits of grain legumes to sorghum under semi-arid conditions in southwestern Zimbabwe

    NARCIS (Netherlands)

    Ncube, B.; Twomlow, S.J.; Wijk, van M.T.; Dimes, J.P.; Giller, K.E.

    2007-01-01

    The productivity and residual benefits of four grain legumes to sorghum (Sorghum bicolor) grown in rotation were measured under semi-arid conditions over three cropping seasons. Two varieties of each of the grain legumes; cowpea (Vigna unguiculata); groundnut (Arachis hypogaea); pigeon pea (Cajanus

  11. Yield potential of pigeon pea cultivars

    Science.gov (United States)

    Yield potential of twelve vegetable pigeon pea (Cajanus cajun) cultivars was evaluated at two locations in eastern Kenya during 2012 and 2013 cropping years. Pigeon pea pod numbers, seeds per pod, seed mass, grain yield and shelling percentage were quantified in three replicated plots, arranged in a...

  12. Isolation and characterization of symbiotic mutants of bradyrhizobium sp. (Arachis) strain NC92: mutants with host-specific defects in nodulation and nitrogen fixation.

    OpenAIRE

    Wilson, K. J.; Anjaiah, V; Nambiar, P T; Ausubel, F M

    1987-01-01

    Random transposon Tn5 mutagenesis of Bradyrhizobium sp. (Arachis) strain NC92, a member of the cowpea cross-inoculation group, was carried out, and kanamycin-resistant transconjugants were tested for their symbiotic phenotype on three host plants: groundnut, siratro, and pigeonpea. Two nodulation (Nod- phenotype) mutants were isolated. One is unable to nodulate all three hosts and appears to contain an insertion in one of the common nodulation genes (nodABCD); the other is a host-specific nod...

  13. Role of classical breeding in improvement of pulse crops

    Directory of Open Access Journals (Sweden)

    N. Nadarajan and Sanjeev Gupta

    2010-07-01

    Full Text Available Classical breeding of many crops has been instrumental for ensuring food security by developing new varieties that are higheryielding,resistant to pests and diseases, drought-resistant or regionally adapted to different environments and growingconditions. A total of 513 cultivars of different pulse crops including chickpea, pigeonpea, mungbean, urdbean, lentil, fieldpeaand rajmash were developed in India itself during last three decades. Adoption of high yielding varieties in early 1980'sincreased the average productivity of the country by 34 % now. Significant achievements have been made in developing shortduration cultivars in almost all pulse crops with incorporation of photo- thermo insensitivity. Genetic resistance for most of thediseases have been identified and incorporated in development of disease resistant cultivars. In fieldpea, a major breakthroughhas been made by developing dwarf and afila plant type which led to increase in yield by 30%. Although cultivar developmenthas traditionally emphasized improvement through pedigree selection, mass –pedigree method and backcross breeding,interspecific hybridization has also received much attention in 1980s. So far eight genotypes in different pulse crops have beendeveloped in the country using interspecific hybridization. In pigeonpea a trait cytoplasmic male sterility has been introducedthrough wild gene introgression. Using this, a hybrid GTH 1 in pigeonpea has been developed in India which has yielded 27%yield superiority than the traditional cultivars.

  14. Unexpected high decomposition of legume residues in dry season soils from tropical coffee plantations and crop lands

    OpenAIRE

    Abera, Girma; Wolde-Meskel, Endalkachew; Bakken, Lars

    2014-01-01

    International audience; Crop residues are essential fertilizer source of low-input farming systems in Sub-Saharan Africa. However, crop residues provide nutrients only if they decompose in the soil. Decomposition is assumed to be very low during the dry season due to the scarcity of water, but there are few quantitative knowledge on decomposition under such conditions. Therefore, we studied the decomposition of legume residues, haricot bean (Phaseolus vulgaris L.), and pigeon pea (Cajanus caj...

  15. Phytomass production and nutrient accumulation by green manure species

    OpenAIRE

    2014-01-01

    Green manuring is recognized as a viable alternative to improve nutrient cycling in soils. The aim of this study was to evaluate the phytomass production and nutrient accumulation in shoots of the summer green manures jack bean [Canavalia ensiformis (L.) DC.], dwarf pigeon pea (Cajanus cajanvar var. Flavus DC.), dwarf mucuna [Mucuna deeringiana (Bort) Merr] and sunn hemp (Crotalaria juncea L.), under nitrogen fertilization and/or inoculation with N-fixing bacteria. A split plo...

  16. Push-pull Strategy with Trap Crops, Neem and Nuclear Polyhedrosis Virus for Insecticide Resistance Management in Helicoverpa armigera (Hubner in Cotton

    Directory of Open Access Journals (Sweden)

    P. Duraimurugan

    2005-01-01

    Full Text Available Insecticide resistance in Helicoverpa armigera (Hubner is a major threat to cotton production in India. The virus infection was found to increase the susceptibility of H. armigera to the insecticides. But, use of Nuclear Polyhedrosis Virus (NPV on a larger scale and on cotton due to leaf alkalinity poses certain practical problems. Hence, studies were carried out to assess the effects of push-pull strategy with trap crops, neem and NPV in cotton for the management of insecticide resistant H. armigera. Field experiments were conducted on cotton (MCU5 with trap crops (okra and pigeonpea and neem was used to diversify the pests to trap crops whereby the control of these pests was assessed with the application of NPV. The preference of H. armigera was towards okra and pigeonpea as a trap crop compared to cotton. Application of NSKE on cotton diversified the H. armigera towards untreated okra and pigeonpea. Push-pull strategy with the conjunctive use of trap crops, restricted application of NSKE on cotton leaving trap crops and restricted application of NPV on trap crops was highly effective in reducing the incidence of H. armigera and damage to fruiting bodies, boll, locule and inter locule basis over cotton sole crop (untreated check. The percent recovery of NPV infected larvae varied from 37.5-47.5, 32.8-39.2 and 14.2-20.2% on okra, pigeon pea and cotton respectively. The synthetic pyrethroids resistance in field survived H. armigera at the end of the season was reduced from 87.5-93.1% to 76.4-84.3%.

  17. Net global warming potential and greenhouse gas intensity of conventional and conservation agriculture system in rainfed semi arid tropics of India

    Science.gov (United States)

    Pratibha, G.; Srinivas, I.; Rao, K. V.; Shanker, Arun K.; Raju, B. M. K.; Choudhary, Deepak K.; Srinivas Rao, K.; Srinivasarao, Ch.; Maheswari, M.

    2016-11-01

    Agriculture has been considered as one of the contributors to greenhouse gas (GHG) emissions and it continues to increase with increase in crop production. Hence development of sustainable agro techniques with maximum crop production, and low global warming potential is need of the hour. Quantifying net global warming potential (NGWP) and greenhouse gas intensity (GHGI) of an agricultural activity is a method to assess the mitigation potential of the activity. But there is dearth of information on NGWP of conservation agriculture under rainfed conditions. Hence in this study two methods such as crop based (NGWPcrop) and soil based (NGWPsoil) were estimated from the data of the experiment initiated in 2009 in rainfed semiarid regions of Hyderabad, India with different tillage practices like conventional tillage (CT), reduced tillage (RT), zero tillage (ZT) and residue retention levels by harvesting at different heights which includes 0, 10 and 30 cm anchored residue in pigeonpea-castor systems. The results of the study revealed that under rainfed conditions CT recorded 24% higher yields over ZT, but CT and RT were on par with each other. However, the yield gap between the tillage treatments is narrowing down over 5 years of study. ZT and RT recorded 26 and 11% lower indirect GHG emissions (emissions from farm operations and input use) over CT, respectively. The percent contribution of CO2 eq. N2O emission is higher to total GHG emissions in both the crops. Both NGWPcrop, NGWPsoil, GHGIcrop, and GHGIsoil based were influenced by tillage and residue treatments. Further, castor grown on pigeonpea residue recorded 20% higher GHG emissions over pigeonpea grown on castor residues. The fuel consumption in ZT was reduced by 58% and 81% as compared to CT in pigeonpea and castor, respectively. Lower NGWP and GHGI based on crop and soil was observed with increase in crop residues and decrease in tillage intensity in both the crops. The results of the study indicate that, there

  18. Diagnosis of nutrient imbalances with vector analysis in agroforestry systems.

    Science.gov (United States)

    Isaac, Marney E; Kimaro, Anthony A

    2011-01-01

    Agricultural intensification has had unintended environmental consequences, including increased nutrient leaching and surface runoff and other agrarian-derived pollutants. Improved diagnosis of on-farm nutrient dynamics will have the advantage of increasing yields and will diminish financial and environmental costs. To achieve this, a management support system that allows for site-specific rapid evaluation of nutrient production imbalances and subsequent management prescriptions is needed for agroecological design. Vector diagnosis, a bivariate model to depict changes in yield and nutritional response simultaneously in a single graph, facilitates identification of nutritional status such as growth dilution, deficiency, sufficiency, luxury uptake, and toxicity. Quantitative data from cocoa agroforestry systems and pigeonpea intercropping trials in Ghana and Tanzania, respectively, were re-evaluated with vector analysis. Relative to monoculture, biomass increase in cocoa ( L.) under shade (35-80%) was accompanied by a 17 to 25% decline in P concentration, the most limiting nutrient on this site. Similarly, increasing biomass with declining P concentrations was noted for pigeonpea [ (L). Millsp.] in response to soil moisture availability under intercropping. Although vector analysis depicted nutrient responses, the current vector model does not consider non-nutrient resource effects on growth, such as ameliorated light and soil moisture, which were particularly active in these systems. We revisit and develop vector analysis into a framework for diagnosing nutrient and non-nutrient interactions in agroforestry systems. Such a diagnostic technique advances management decision-making by increasing nutrient precision and reducing environmental issues associated with agrarian-derived soil contamination.

  19. Effect of Integrated Water-Nutrient Management Strategies on Soil Erosion Mediated Nutrient Loss and Crop Productivity in Cabo Verde Drylands.

    Science.gov (United States)

    Baptista, Isaurinda; Ritsema, Coen; Geissen, Violette

    2015-01-01

    Soil erosion, runoff and related nutrient losses are a big risk for soil fertility in Cabo Verde drylands. In 2012, field trials were conducted in two agro-ecological zones to evaluate the effects of selected techniques of soil-water management combined with organic amendments (T1: compost/manure + soil surfactant; T2: compost/animal or green manure + pigeon-pea hedges + soil surfactant; T3: compost/animal or green manure + mulch + pigeon-pea hedges) on nitrogen (N) and phosphorus (P) losses in eroded soil and runoff and on crop yields. Three treatments and one control (traditional practice) were tested in field plots at three sites with a local maize variety and two types of beans. Runoff and eroded soil were collected after each erosive rain, quantified, and analysed for NO3-N and PO4-P concentrations. In all treatments runoff had higher concentrations of NO3-N (2.20-4.83 mg L-1) than of PO4-P (0.02-0.07 mg L-1), and the eroded soil had higher content of PO4-P (5.27-18.8 mg g-1) than of NO3-N (1.30-8.51 mg g-1). The control had significantly higher losses of both NO3-N (5.4, 4.4 and 19 kg ha-1) and PO4-P (0.2, 0.1 and 0.4 kg ha-1) than the other treatments. T3 reduced soil loss, runoff and nutrient losses to nearly a 100% while T1 and T2 reduced those losses from 43 to 88%. The losses of NO3-N and PO4-P were highly correlated with the amounts of runoff and eroded soil. Nutrient losses from the applied amendments were low (5.7% maximum), but the losses in the control could indicate long-term nutrient depletion in the soil (19 and 0.4 kg ha-1 of NO3-N and PO4-P, respectively). T1-T3 did not consistently increase crop yield or biomass in all three sites, but T1 increased both crop yield and biomass. We conclude that T3 (combining crop-residue mulch with organic amendment and runoff hedges) is the best treatment for steep slope areas but, the pigeon-pea hedges need to be managed for higher maize yield. T1 (combining organic amendment with soil surfactant) could be a

  20. Potential Uses of Wild Germplasms of Grain Legumes for Crop Improvement

    Science.gov (United States)

    Muñoz, Nacira; Liu, Ailin; Kan, Leo; Li, Man-Wah; Lam, Hon-Ming

    2017-01-01

    Challenged by population increase, climatic change, and soil deterioration, crop improvement is always a priority in securing food supplies. Although the production of grain legumes is in general lower than that of cereals, the nutritional value of grain legumes make them important components of food security. Nevertheless, limited by severe genetic bottlenecks during domestication and human selection, grain legumes, like other crops, have suffered from a loss of genetic diversity which is essential for providing genetic materials for crop improvement programs. Illustrated by whole-genome-sequencing, wild relatives of crops adapted to various environments were shown to maintain high genetic diversity. In this review, we focused on nine important grain legumes (soybean, peanut, pea, chickpea, common bean, lentil, cowpea, lupin, and pigeonpea) to discuss the potential uses of their wild relatives as genetic resources for crop breeding and improvement, and summarized the various genetic/genomic approaches adopted for these purposes. PMID:28165413

  1. Bacterial Molecular Signals in the Sinorhizobium fredii-Soybean Symbiosis

    Directory of Open Access Journals (Sweden)

    Francisco J. López-Baena

    2016-05-01

    Full Text Available Sinorhizobium (Ensifer fredii (S. fredii is a rhizobial species exhibiting a remarkably broad nodulation host-range. Thus, S. fredii is able to effectively nodulate dozens of different legumes, including plants forming determinate nodules, such as the important crops soybean and cowpea, and plants forming indeterminate nodules, such as Glycyrrhiza uralensis and pigeon-pea. This capacity of adaptation to different symbioses makes the study of the molecular signals produced by S. fredii strains of increasing interest since it allows the analysis of their symbiotic role in different types of nodule. In this review, we analyze in depth different S. fredii molecules that act as signals in symbiosis, including nodulation factors, different surface polysaccharides (exopolysaccharides, lipopolysaccharides, cyclic glucans, and K-antigen capsular polysaccharides, and effectors delivered to the interior of the host cells through a symbiotic type 3 secretion system.

  2. Potential Uses of Wild Germplasms of Grain Legumes for Crop Improvement

    Directory of Open Access Journals (Sweden)

    Nacira Muñoz

    2017-02-01

    Full Text Available Challenged by population increase, climatic change, and soil deterioration, crop improvement is always a priority in securing food supplies. Although the production of grain legumes is in general lower than that of cereals, the nutritional value of grain legumes make them important components of food security. Nevertheless, limited by severe genetic bottlenecks during domestication and human selection, grain legumes, like other crops, have suffered from a loss of genetic diversity which is essential for providing genetic materials for crop improvement programs. Illustrated by whole-genome-sequencing, wild relatives of crops adapted to various environments were shown to maintain high genetic diversity. In this review, we focused on nine important grain legumes (soybean, peanut, pea, chickpea, common bean, lentil, cowpea, lupin, and pigeonpea to discuss the potential uses of their wild relatives as genetic resources for crop breeding and improvement, and summarized the various genetic/genomic approaches adopted for these purposes.

  3. Potential Uses of Wild Germplasms of Grain Legumes for Crop Improvement.

    Science.gov (United States)

    Muñoz, Nacira; Liu, Ailin; Kan, Leo; Li, Man-Wah; Lam, Hon-Ming

    2017-02-04

    Challenged by population increase, climatic change, and soil deterioration, crop improvement is always a priority in securing food supplies. Although the production of grain legumes is in general lower than that of cereals, the nutritional value of grain legumes make them important components of food security. Nevertheless, limited by severe genetic bottlenecks during domestication and human selection, grain legumes, like other crops, have suffered from a loss of genetic diversity which is essential for providing genetic materials for crop improvement programs. Illustrated by whole-genome-sequencing, wild relatives of crops adapted to various environments were shown to maintain high genetic diversity. In this review, we focused on nine important grain legumes (soybean, peanut, pea, chickpea, common bean, lentil, cowpea, lupin, and pigeonpea) to discuss the potential uses of their wild relatives as genetic resources for crop breeding and improvement, and summarized the various genetic/genomic approaches adopted for these purposes.

  4. Long term effects of irrigation with petrochemical industry wastewater

    Energy Technology Data Exchange (ETDEWEB)

    Aziz, O.; Inam, A.; Samiullah; Siddiqi, R.H. [Aligarh Muslim Univ. (India)

    1996-11-01

    Split plot designed field trials were conducted during 1988-1995 to study the long term effects of petrochemical industry wastewater on six crops and agricultural soils. It was observed that wastewater irrigation resulted in increased seed yield of all the crops selected, viz. wheat, triticale, chickpea, lentil and pigeonpea, except summer moong which showed a decrease in seed yield. Soil receiving the wastewater showed no significant changes in pH, total organic carbon, electrical conductivity, cation exchange capacity, micro- and macro-nutrients and SAR. Thus, it may be concluded that treated refinery wastewater met the irrigational quality requirements as its physico-chemical characteristics were within the permissible limits. The same could be said for the accumulation of heavy metals in the soil as well as in the grains making the latter safe for human consumption. 28 refs., 5 figs., 2 tabs.

  5. Transcriptome Sequencing of Lima Bean (Phaseolus lunatus to Identify Putative Positive Selection in Phaseolus and Legumes

    Directory of Open Access Journals (Sweden)

    Fengqi Li

    2015-07-01

    Full Text Available The identification of genes under positive selection is a central goal of evolutionary biology. Many legume species, including Phaseolus vulgaris (common bean and Phaseolus lunatus (lima bean, have important ecological and economic value. In this study, we sequenced and assembled the transcriptome of one Phaseolus species, lima bean. A comparison with the genomes of six other legume species, including the common bean, Medicago, lotus, soybean, chickpea, and pigeonpea, revealed 15 and 4 orthologous groups with signatures of positive selection among the two Phaseolus species and among the seven legume species, respectively. Characterization of these positively selected genes using Non redundant (nr annotation, gene ontology (GO classification, GO term enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG pathway analyses revealed that these genes are mostly involved in thylakoids, photosynthesis and metabolism. This study identified genes that may be related to the divergence of the Phaseolus and legume species. These detected genes are particularly good candidates for subsequent functional studies.

  6. A protocol for high-quality genomic DNA extraction from legumes.

    Science.gov (United States)

    Agbagwa, I O; Datta, S; Patil, P G; Singh, P; Nadarajan, N

    2012-12-19

    Current DNA extraction protocols, which require liquid nitrogen, lyophilization and considerable infrastructure in terms of instrumentation, often impede the application of biotechnological tools in less researched crops in laboratories in developing countries. We modified and optimized the existing CTAB method for plant genomic DNA extraction by avoiding liquid nitrogen usage and lyophilization. DNA was extracted directly from freshly harvested leaves ground in pre-heated CTAB buffer. Chloroform:isoamyl alcohol (24:1) and RNase treatments followed by single-purification step decontaminated the samples thereby paving way for selective extraction of DNA. High molecular weight DNA yield in the range of 328 to 4776 ng/μL with an average of 1459 ng/μL was obtained from 45 samples of cultivated and wild Cajanus species. With an absorbance ratio at 260 to 280 nm, a range of 1.66 to 2.20, and a mean of 1.85, very low levels of protein and polysaccharide contamination were recorded. Forty samples can be extracted daily at a cost between 1.8 and US$2.0 per plant sample. This modified method is suitable for most plants especially members of the Leguminosae. Apart from Cajanus, it has been extensively applied in DNA extraction from Cicer and Vigna species.

  7. Achievements and prospects of genomics-assisted breeding in three legume crops of the semi-arid tropics.

    Science.gov (United States)

    Varshney, Rajeev K; Mohan, S Murali; Gaur, Pooran M; Gangarao, N V P R; Pandey, Manish K; Bohra, Abhishek; Sawargaonkar, Shrikant L; Chitikineni, Annapurna; Kimurto, Paul K; Janila, Pasupuleti; Saxena, K B; Fikre, Asnake; Sharma, Mamta; Rathore, Abhishek; Pratap, Aditya; Tripathi, Shailesh; Datta, Subhojit; Chaturvedi, S K; Mallikarjuna, Nalini; Anuradha, G; Babbar, Anita; Choudhary, Arbind K; Mhase, M B; Bharadwaj, Ch; Mannur, D M; Harer, P N; Guo, Baozhu; Liang, Xuanqiang; Nadarajan, N; Gowda, C L L

    2013-12-01

    Advances in next-generation sequencing and genotyping technologies have enabled generation of large-scale genomic resources such as molecular markers, transcript reads and BAC-end sequences (BESs) in chickpea, pigeonpea and groundnut, three major legume crops of the semi-arid tropics. Comprehensive transcriptome assemblies and genome sequences have either been developed or underway in these crops. Based on these resources, dense genetic maps, QTL maps as well as physical maps for these legume species have also been developed. As a result, these crops have graduated from 'orphan' or 'less-studied' crops to 'genomic resources rich' crops. This article summarizes the above-mentioned advances in genomics and genomics-assisted breeding applications in the form of marker-assisted selection (MAS) for hybrid purity assessment in pigeonpea; marker-assisted backcrossing (MABC) for introgressing QTL region for drought-tolerance related traits, Fusarium wilt (FW) resistance and Ascochyta blight (AB) resistance in chickpea; late leaf spot (LLS), leaf rust and nematode resistance in groundnut. We critically present the case of use of other modern breeding approaches like marker-assisted recurrent selection (MARS) and genomic selection (GS) to utilize the full potential of genomics-assisted breeding for developing superior cultivars with enhanced tolerance to various environmental stresses. In addition, this article recommends the use of advanced-backcross (AB-backcross) breeding and development of specialized populations such as multi-parents advanced generation intercross (MAGIC) for creating new variations that will help in developing superior lines with broadened genetic base. In summary, we propose the use of integrated genomics and breeding approach in these legume crops to enhance crop productivity in marginal environments ensuring food security in developing countries.

  8. Tylenchida associated with different crops in Sennar State (Sudan).

    Science.gov (United States)

    Elbadri, G A; Bert, W; Geraert, E

    2001-01-01

    A study was done on the taxonomy and morphology of plant parasitic nematodes (Tylenchida) found in Sennar State (Sudan). Sixty samples of different crops were collected in the sugarcane area. Thirty samples originated from soil around the roots of Saccharum officinarum (sugarcane) from different ratoons and thirty samples were collected from other crops (Mangifera indica; Citrus limon; Citrus aurantifolia; Citrus paradisi; Citrus sinensis, Phoenix dactylifera, Musa sapentium; Cassia italica, Capsicum annuum, Sorghum bicolor, Sorghum sudanensis, Gossypium barbadense, Ficus nitida, Khaya senegalensis, Eucalyptus microtheca, Acacia nilotica, Acacia seyal, Azardichta indica, Cajanus cajana, Caltropsis spp. and Liguster ovalifolium). Seven species belonging to seven different genera of Tylenchida were identified: Paratrophurus lobatus, Scutellonema clathricaudatum, Hoplolaimus aegypti and Filenchus cylindricus. Helicotylenchus plumariae, Pratylenchus thornei and Malenchus andrassyi are new records for Sudan. These seven species were compared with the descriptions given in the literature and differences and variations were discussed. Additional morphological data were described by means of SEM microscopy.

  9. Biodegradation of spent engine oil by bacteria isolated from the rhizosphere of legumes grown in contaminated soil

    Directory of Open Access Journals (Sweden)

    HY Ismail

    2014-05-01

    Full Text Available Biodegradation of spent engine oil (SEO by bacteria isolated from the rhizosphere of Cajan cajan and Lablab purpureus was investigated. It was with a view to determining most efficient bacterial species that could degrade SEO in phytoremediation studies. Hydrocarbon degrading bacteria were isolated and identified by enrichment culture technique using oil agar supplemented with 0.1% v/v SEO. Total heterotrophic and oil utilizing bacterial count showed the occurrence of large number of bacteria predominantly in the rhizosphere soil, ranging between 54×108 - 144×108 CFU/g and 4×108- 96×108 CFU/g respectively. Percentage of oil utilizing bacteria ranged between 0% (uncontaminated non rhizosphere soil to 76% (contaminated rhizosphere. Turbidimetrically, five bacterial species namely Pseudomonas putrefacience CR33, Klebsiella pneumonia CR23, Pseudomonas alcaligenes LR14, Klebsiella aerogenes CR21, and Bacillus coagulans CR31 were shown to grow maximally and degraded the oil at the rate of 68%, 62%, 59%, 58%and 45% respectively. Chromatographic analysis using GC-MS showed the presence of lower molecular weight hydrocarbons in the residual oil (indicating degradation after 21 days, whereas the undegraded oil (control had higher molecular weight hydrocarbons after the same period. The species isolated were shown to have high ability of SEO biodegradation and therefore could be important tools in ameliorating SEO contaminated soil. DOI: http://dx.doi.org/10.3126/ije.v3i2.10515 International Journal of the Environment Vol.3(2 2014: 63-75

  10. A BRIEF REVIEW ON "MOLECULAR DETECTION AND CHARACTERIZATION OF YELLOW MOSAIC VIRUS (YMV INFECTING BLACKGRAM"

    Directory of Open Access Journals (Sweden)

    S.Obaiah

    2013-12-01

    Full Text Available Blackgram (Vigna mungo (L. Hepper is one of the major pulse crops of the tropics and sub tropics. It is the third major pulse crop cultivated in the Indian subcontinent. Pulses and grain legumes are major sources of dietary protein. These crops are subjected to yellow mosaic and golden mosaic diseases caused by white fly transmitted geminiviruses (WTG’s or begomovirus. Of these viruses, mungbean yellow mosaic virus (MYMV is an important one, and it infects five major leguminous species, such as blackgram, greengram, Frenchbean, pigeonpea and soybean causing an annual yield loss of about US $ 300 million (Varma et al., 1992. The MYMV causes 85-100 per cent yield loss in the plants that are infected at the seedling stage (Nene, 1973.MYMV was first observed in Delhi in the late fifties (Nariani, 1960. Virus particles were first observed by Thongmeearkom et al. (1981 and purified by Honda et al. (1983. Hence the characterisation of Yellow Mosaic Virus is essential to study the variability and to identify any new strains/ variants of YMV prevalent in India and Abroad at molecular level for developing the new resistant genotypes.

  11. Genome-Wide Identification, Characterization, and Expression Analysis of Small RNA Biogenesis Purveyors Reveal Their Role in Regulation of Biotic Stress Responses in Three Legume Crops

    Directory of Open Access Journals (Sweden)

    Rajeev K. Varshney

    2017-04-01

    Full Text Available Biotic stress in legume crops is one of the major threats to crop yield and productivity. Being sessile organisms, plants have evolved a myriad of mechanisms to combat different stresses imposed on them. One such mechanism, deciphered in the last decade, is small RNA (sRNA mediated defense in plants. Small RNAs (sRNAs have emerged as one of the major players in gene expression regulation in plants during developmental stages and under stress conditions. They are known to act both at transcriptional and post-transcriptional levels. Dicer-like (DCL, Argonaute (AGO, and RNA dependent RNA polymerase (RDR constitute the major components of sRNA biogenesis machinery and are known to play a significant role in combating biotic and abiotic stresses. This study is, therefore, focused on identification and characterization of sRNA biogenesis proteins in three important legume crops, namely chickpea, pigeonpea, and groundnut. Phylogenetic analysis of these proteins between legume species classified them into distinct clades and suggests the evolutionary conservation of these genes across the members of Papillionidoids subfamily. Variable expression of sRNA biogenesis genes in response to the biotic stresses among the three legumes indicate the possible existence of specialized regulatory mechanisms in different legumes. This is the first ever study to understand the role of sRNA biogenesis genes in response to pathogen attacks in the studied legumes.

  12. Rapid and sensitive diagnoses of dry root rot pathogen of chickpea (Rhizoctonia bataticola (Taub.) Butler) using loop-mediated isothermal amplification assay.

    Science.gov (United States)

    Ghosh, Raju; Tarafdar, Avijit; Sharma, Mamta

    2017-02-20

    Dry root rot (DRR) caused by the fungus Rhizoctonia bataticola (Taub.) Butler, is an emerging disease in chickpea. The disease is often mistaken with other root rots like Fusarium wilt, collar rot and black root rot in chickpea. Therefore, its timely and specific detection is important. Current detection protocols are either based on mycological methods or on protocols involving DNA amplification by polymerase chain reaction (PCR). Here we report the rapid and specific detection of R. bataticola using loop-mediated isothermal amplification (LAMP) assay targeting fungal specific 5.8S rDNA sequence for visual detection of R. bataticola. The reaction was optimized at 63 °C for 75 min using minimum 10 fg of DNA. After adding SYBR Green I in LAMP products, the amplification was found to be highly specific in all the 94 isolates of R. bataticola collected from diverse geographical regions as well as DRR infected plants and sick soil. No reaction was found in other pathogenic fungi infecting chickpea (Fusarium oxysporum f. sp. ciceris, Rhizoctonia solani, Sclerotium rolfsii and Fusarium solani) and pigeonpea (Fusarium udum and Phytophthora cajani). The standardised LAMP assay with its simplicity, rapidity and specificity is very useful for the visual detection of this emerging disease in chickpea.

  13. Influence of Bacillus spp. strains on seedling growth and physiological parameters of sorghum under moisture stress conditions.

    Science.gov (United States)

    Grover, Minakshi; Madhubala, R; Ali, Sk Z; Yadav, S K; Venkateswarlu, B

    2014-09-01

    Microorganisms isolated from stressed ecosystem may prove as ideal candidates for development of bio-inoculants for stressed agricultural production systems. In the present study, moisture stress tolerant rhizobacteria were isolated from the rhizosphere of sorghum, pigeonpea, and cowpea grown under semiarid conditions in India. Four isolates KB122, KB129, KB133, and KB142 from sorghum rhizosphere exhibited plant growth promoting traits and tolerance to salinity, high temperature, and moisture stress. These isolates were identified as Bacillus spp. by 16S rDNA sequence analysis. The strains were evaluated for growth promotion of sorghum seedlings under two different moisture stress conditions (set-I, continuous 50% soil water holding capacity (WHC) throughout the experiment and set-II, 75% soil WHC for 27 days followed by no irrigation for 5 days) under greenhouse conditions. Plate count and scanning electron microscope studies indicated successful root surface colonization by inoculated bacteria. Plants inoculated with Bacillus spp. strains showed better growth in terms of shoot length and root biomass with dark greenish leaves due to high chlorophyll content while un-inoculated plants showed rolling of the leaves, stunted appearance, and wilting under both stress conditions. Inoculation also improved leaf relative water content and soil moisture content. However, variation in proline and sugar content in the different treatments under two stress conditions indicated differential effect of microbial treatments on plant physiological parameters under stress conditions.

  14. Antibegomoviral activity of the agrobacterial virulence protein VirE2.

    Science.gov (United States)

    Sunitha, Sukumaran; Marian, Dolly; Hohn, Barbara; Veluthambi, Karuppannan

    2011-12-01

    Mungbean yellow mosaic geminivirus (MYMV) causes severe yellow mosaic disease in blackgram, mungbean, Frenchbean, pigeonpea, soybean and mothbean. We attempted to induce resistance against this virus using the transcriptional activator protein gene deleted in the C-terminal activation domain (TrAP-∆AD) and Agrobacterium tumefaciens virE2. MYMV is known to replicate in agroinoculated tobacco leaf discs. Three transgenic tobacco plants which harboured a truncated MYMV transcriptional activator protein gene and two tobacco plants transformed with the octopine type A. tumefaciens virE2 gene were agroinoculated with an A. tumefaciens strain which harboured the partial dimers of both DNA A and DNA B of MYMV. The level of viral DNA accumulation in leaf discs of transgenic plants correlated inversely to the level of the MYMV TrAP-∆AD transcript. Two VirE2-transgenic plants, which complemented tumorigenesis of a virE2 mutant A. tumefaciens strain, effectively reduced MYMV DNA accumulation in the leaf disc agroinoculation assay.

  15. Effect of carbon substrates on rock phosphate solubilization by bacteria from composts and macrofauna.

    Science.gov (United States)

    Hameeda, B; Reddy, Y Harish Kumar; Rupela, O P; Kumar, G N; Reddy, Gopal

    2006-10-01

    Five of the 207 isolates from different composts, farm waste compost (FWC), rice straw compost (RSC), Gliricidia vermicompost (GVC), and macrofauna, showed rock phosphate (RP) solubilization in buffered medium in plate culture. When tested in RP broth medium, all five strains, Enterobacter cloacae EB 27, Serratia marcescens EB 67, Serratia sp. EB 75, Pseudomonas sp. CDB 35, and Pseudomonas sp. BWB 21, showed gluconic acid production and solubilized RP. Based on cellulose-degrading and P-solubilizing ability, two strains were selected for further studies. In the presence of different carbon sources, both strains showed a drop in pH and solubilized RP. P released was maximum with glucose (1212 and 522 micromol) and minimum with cellobiose (455 and 306 micromol) by S. marcescens EB 67 and Pseudomonas sp. CDB 35, respectively. Glucose dehydrogenase (GDH) activity was 63 and 77% with galactose and 35 and 46% with cellobiose when compared to glucose (100%) by EB 67 and CDB 35, respectively. Both strains solubilized RP in the presence of different crop residues. EB 67 and CDB 35 showed maximum cellulase activity (0.027 units) in the presence of rice straw and a mixture of rice straw and root. P solubilized from RP in the presence of pigeonpea root was 134 and 140 micromol with EB 67 and CDB 35. Significantly, these bacteria isolated from composts and macrofauna solubilized rock phosphate in the presence of various pure carbon substrates and crop residues and their importance in soil/rhizosphere conditions is discussed.

  16. Tropical food legumes: virus diseases of economic importance and their control.

    Science.gov (United States)

    Hema, Masarapu; Sreenivasulu, Pothur; Patil, Basavaprabhu L; Kumar, P Lava; Reddy, Dodla V R

    2014-01-01

    Diverse array of food legume crops (Fabaceae: Papilionoideae) have been adopted worldwide for their protein-rich seed. Choice of legumes and their importance vary in different parts of the world. The economically important legumes are severely affected by a range of virus diseases causing significant economic losses due to reduction in grain production, poor quality seed, and costs incurred in phytosanitation and disease control. The majority of the viruses infecting legumes are vectored by insects, and several of them are also seed transmitted, thus assuming importance in the quarantine and in the epidemiology. This review is focused on the economically important viruses of soybean, groundnut, common bean, cowpea, pigeonpea, mungbean, urdbean, chickpea, pea, faba bean, and lentil and begomovirus diseases of three minor tropical food legumes (hyacinth bean, horse gram, and lima bean). Aspects included are geographic distribution, impact on crop growth and yields, virus characteristics, diagnosis of causal viruses, disease epidemiology, and options for control. Effectiveness of selection and planting with virus-free seed, phytosanitation, manipulation of crop cultural and agronomic practices, control of virus vectors and host plant resistance, and potential of transgenic resistance for legume virus disease control are discussed.

  17. Genome-wide analysis of homeobox gene family in legumes: identification, gene duplication and expression profiling.

    Science.gov (United States)

    Bhattacharjee, Annapurna; Ghangal, Rajesh; Garg, Rohini; Jain, Mukesh

    2015-01-01

    Homeobox genes encode transcription factors that are known to play a major role in different aspects of plant growth and development. In the present study, we identified homeobox genes belonging to 14 different classes in five legume species, including chickpea, soybean, Medicago, Lotus and pigeonpea. The characteristic differences within homeodomain sequences among various classes of homeobox gene family were quite evident. Genome-wide expression analysis using publicly available datasets (RNA-seq and microarray) indicated that homeobox genes are differentially expressed in various tissues/developmental stages and under stress conditions in different legumes. We validated the differential expression of selected chickpea homeobox genes via quantitative reverse transcription polymerase chain reaction. Genome duplication analysis in soybean indicated that segmental duplication has significantly contributed in the expansion of homeobox gene family. The Ka/Ks ratio of duplicated homeobox genes in soybean showed that several members of this family have undergone purifying selection. Moreover, expression profiling indicated that duplicated genes might have been retained due to sub-functionalization. The genome-wide identification and comprehensive gene expression profiling of homeobox gene family members in legumes will provide opportunities for functional analysis to unravel their exact role in plant growth and development.

  18. Survival and preference of cotton boll weevil adults for alternative food sources

    Directory of Open Access Journals (Sweden)

    M. Pimenta

    Full Text Available Abstract Plants that have potential as alternative food source (floral nectar, pollen and plant tissues to the boll weevil during the intercropping season were evaluated considering the prevalent conditions of Cerrado in the Central Brazil. Initially, we tested the nutritional adequacy for the survival of the insect of flower resource (pollen and nectar provided by eight plant species (fennel, mexican sunflower, castor bean, okra, hibiscus, sorghum, pigeonpea and sunn hemp. Subsequently, we tested if the resources provided by the selected plants continued to be exploited by the boll weevil in the presence of cotton plant, its main food source average longevity of boll weevil adults was significantly longer when they were fed on hibiscus’ flowers (166.6 ± 74.4 and okra flowers (34.7 ± 28.9 than when they fed on flowers of other six species. Subsequently, the preference of the boll weevil in the use of resources was compared between okra or hibiscus and cotton plants, in dual choice experiments. Boll weevils preferred plants of the three species in the reproductive stages than those in vegetative stages. Although the cotton plant in the reproductive stage was the most preferred plant of all, boll weevils preferred flowering okra and hibiscus than cotton at the vegetative stage.

  19. Diversity and aggregation patterns of plant species in a grass community

    Directory of Open Access Journals (Sweden)

    Ran Li

    2014-09-01

    Full Text Available Both composition and aggregation patterns of species in a community are the outcome of community self-organizing. In this paper we conducted analysis on species diversity and aggregation patterns of plant species in a grass community, Zhuhai, China. According to the sampling survey, in total of 47 plant species, belonging to 16 families, were found. Compositae had 10 species (21.3%, seconded by Gramineae (9 species, 19.1%, Leguminosae (6 species, 12.8%, Cyperaceae (4 species, 8.5%, and Malvaceae (3 species, 6.4%. The results revealed that the means of aggregation indices Iδ, I and m*/m were 21.71, 15.71 and 19.89 respectively and thus individuals of most of plant species strongly followed aggregative distribution. Iwao analysis indicated that both individuals of all species and clumps of all individuals of all species followed aggregative distribution. Taylor's power law indicated that individuals of all species followed aggregative distribution and aggregation intensity strengthened as the increase of mean density. We held that the strong aggregation intensity of a species has been resulted from the strong adaptation ability to the environment, the strong interspecific competition ability and the earlier establishment of the species. Fitting goodness of the mean, I, Iδ, m*/m with probability distributions demonstrated that the mean (density, I, Iδ, and m*/m over all species followed Weibull distribution rather than normal distribution. Lophatherum gracile, Paederia scandens (Lour. Merr., Eleusine indica, and Alternanthera philoxeroides (Mart. Griseb. were mostly aggregative, and Oxalis sp., Eleocharis plantagineiformis, Vernonia cinerea (L. Less., and Sapium sebiferum (L. Roxb, were mostly uniform in the spatial distribution. Importance values (IV showed that Cynodon dactylon was the most important species, seconded by Desmodium triflorum (L. DC., Cajanus scarabaeoides (L. Benth., Paspalum scrobiculatum L., and Rhynchelytrum repens. Oxalis

  20. Evaluation of three manually operated weeding devices.

    Science.gov (United States)

    Tewari, V K; Datta, R K; Murthy, A S

    1991-04-01

    Performance of three manually operated weeders was evaluated from ergonomics and mechanical considerations. Three operators were selected for laboratory and field trials; they represented the 5th, 50th and 95th percentiles of the operator population. Laboratory tests were conducted in a psychometric chamber to study physiological response under varying load and environmental conditions. From the data, relationships between energy expenditure rate and oxygen consumption rate vs heart rate were established. Field tests were carried out with the three weeders in a farm with Arhar crop (Cajannus Cajan L.) during August-September, when the average ambient temperature and relative humidity were 36 degrees C and 82% respectively. The results of this investigation indicated that weeding with the indigenous tools of a 'khurpi' and a spade and with the improved tool (3-tine hoe) could be rated as 'moderately heavy' work. However, a 'khurpi' demanded less energy expenditure than a 3-tine hoe followed by a spade. The squatting posture with a 'khurpi' appeared to be more comfortable than the standing posture with about 145 degrees erect position for the 3-tine hoe, followed by the standing posture with about 108 degrees erect position with the spade. For consideration of higher output, the order was spade, 3-tine hoe and 'khurpi', For weeding efficiency the trend was, however, just the reverse.

  1. Effect of fly ash on VAM formation and growth response of pulse crops infested with Glomus aggregatum in sterile soil

    Energy Technology Data Exchange (ETDEWEB)

    Reddy, C.N.; Garampalli, H.R. [Gulbarga University, Gulbarga (India). Dept. of P.G. Studies and Research in Botany

    2002-07-01

    The effect of flyash amendment at 3 concentrations (10%, 20% and 30%) on the infectivity and efficacy of Glomus aggregatum was studied by conducting a pot culture experiment with sterile low fertile soil using pigeon pea (Cajanus cagan (L.) Millsp.) Cv. Maruti and Chick pea (Cicer ariteinum L.) Cv. Annigeri, the two pulse crop cultivars of this region. It is evident from the present investigation that the percent vesicular arbuscular mycorrhiza, VAM colonization in both the crops significantly decreased with the increase of flyash content in the soil. The formation of VAM fungal structures (vesicles and arbuscules) inside the host root was also found completely suppressed at higher concentrations of flyash. The effectiveness of G. aggregatum under the influence of flyash was found significantly affected as compared to control, when judged by the growth response of pigeon pea. However in chickpea VAM association could slightly increase the growth over its control. Flyash amendment alone also has shown positive influence on the growth of both the crops over their controls (without VAM association). This influence of flyash amendment together with the usefulness of VAM fungi, as bioremediation agents can be exploited suitably in reclamation of waste lands and soils overburdened with flyash. 40 refs., 3 tabs.

  2. 岩溶峰丛山地几种植物营养价值及饲喂效果研究%Studies on the nutrition value and feeding effect of several forage cultivars in karst mountainous region

    Institute of Scientific and Technical Information of China (English)

    蓝芙宁; 蒋忠诚; 谢运球; 张敏

    2008-01-01

    通过为期3年的试验,对所栽培的银合欢Leucaena leucocephala、任豆树Zenia insignis、木豆Cajanus cauan和桂牧1号Pennisetum purpureum cv.Guimu-1 4种植物的营养价值及饲喂效果进行了研究.结果表明,银合欢粗蛋白含量最高,适口性优,补饲效果显著,再生能力强;任豆树粗脂肪含量最高,木豆粗蛋白含量较高.任豆树和木豆的适口性中等,补饲效果明显,再生能力较强;桂牧1号粗纤维含量最高,适口性优,补饲效果显著,再生能力强.4种植物的营养价值高,补饲效果理想,具有较高的经济价值,是适宜在我国南方岩溶峰丛山地推广种植的优良牧草品种.

  3. 干热河谷草地灌草组合模式的研究%Study on shrub-grass combined mode in the hot-arid valley grassland

    Institute of Scientific and Technical Information of China (English)

    毕玉芬; 姜华; 许岳飞

    2009-01-01

    以7种灌草的8种组合模式为研究对象,以退化灌草地为对照,对各种组合模式灌草的保存率、水土保持功能及地上生物量进行了研究,结果表明:木豆Cajanus cajan、银合欢Leucaena leucocephala以及禾本科的非洲狗尾草Setaria sphacelata cv. Narok和豆科大翼豆Macroptilum atropurpureum的各种生物学性状表现优异,而禾本科草高羊茅Festuca arundinacea cv. Fine Lawn以及豆科的白三叶Trifolium repens cv. Haifa、杂三叶T. hybridum不能很好地在金沙江干热河谷山地生长,不能越过干热季;通过不同灌草组合的水土保持功能和地上生物量的研究,筛选出木豆+非洲狗尾草+高羊茅+大翼豆+白三叶+杂三叶为干热河谷最佳灌草组合模式.

  4. Phytomass production and nutrient accumulation by green manure species

    Directory of Open Access Journals (Sweden)

    José Carlos Soares Mangaravite

    2014-10-01

    Full Text Available Green manuring is recognized as a viable alternative to improve nutrient cycling in soils. The aim of this study was to evaluate the phytomass production and nutrient accumulation in shoots of the summer green manures jack bean [Canavalia ensiformis (L. DC.], dwarf pigeon pea (Cajanus cajanvar var. Flavus DC., dwarf mucuna [Mucuna deeringiana (Bort Merr] and sunn hemp (Crotalaria juncea L., under nitrogen fertilization and/or inoculation with N-fixing bacteria. A split plot design was arranged with the four Fabaceae species as main plots and nitrogen fertilization (with and without and inoculation with diazotrophic bacteria (with and without as the subplots, in a 2² factorial. The experiment was arranged as a randomized complete block design with four replications. In the conditions of this trial, the sunn hemp had the highest production of shoot phytomass (12.4 Mg ha-1 and nutrient accumulation, while the dwarf mucuna had the lowest production of shoot phytomass (3.9 Mg ha-1 and nutrient accumulation. The results showed no effect of nitrogen fertilization or inoculation with N-fixing bacteria on the production of shoot phytomass and nutrient accumulation, except for inoculation without nitrogen fertilization, resulting in greater P accumulation (p <0.05 in the sunn hemp and greater Zn and Mn accumulation in the dwarf mucuna. These findings indicate that N fertilization or inoculation with N2-fixing bacteria for Fabaceae are low efficiency practices in the edaphoclimatic conditions of this study.

  5. Genomic survey, gene expression analysis and structural modeling suggest diverse roles of DNA methyltransferases in legumes.

    Directory of Open Access Journals (Sweden)

    Rohini Garg

    Full Text Available DNA methylation plays a crucial role in development through inheritable gene silencing. Plants possess three types of DNA methyltransferases (MTases, namely Methyltransferase (MET, Chromomethylase (CMT and Domains Rearranged Methyltransferase (DRM, which maintain methylation at CG, CHG and CHH sites. DNA MTases have not been studied in legumes so far. Here, we report the identification and analysis of putative DNA MTases in five legumes, including chickpea, soybean, pigeonpea, Medicago and Lotus. MTases in legumes could be classified in known MET, CMT, DRM and DNA nucleotide methyltransferases (DNMT2 subfamilies based on their domain organization. First three MTases represent DNA MTases, whereas DNMT2 represents a transfer RNA (tRNA MTase. Structural comparison of all the MTases in plants with known MTases in mammalian and plant systems have been reported to assign structural features in context of biological functions of these proteins. The structure analysis clearly specified regions crucial for protein-protein interactions and regions important for nucleosome binding in various domains of CMT and MET proteins. In addition, structural model of DRM suggested that circular permutation of motifs does not have any effect on overall structure of DNA methyltransferase domain. These results provide valuable insights into role of various domains in molecular recognition and should facilitate mechanistic understanding of their function in mediating specific methylation patterns. Further, the comprehensive gene expression analyses of MTases in legumes provided evidence of their role in various developmental processes throughout the plant life cycle and response to various abiotic stresses. Overall, our study will be very helpful in establishing the specific functions of DNA MTases in legumes.

  6. Energy dynamics in Populus deltoides G{sub 3} Marsh agroforestry systems in eastern India

    Energy Technology Data Exchange (ETDEWEB)

    Chaturvedi, O.P. [National Research Centre for Agroforestry, Jhansi (India); Das, D.K. [Rajendra Agricultural Univ., Dept. of Forestry, Bihar (India)

    2005-08-01

    Energy efficiency of Populus deltoides G{sub 3} Marsh agroforestry of a 3-year-old system with intercropping of maize-wheat in crop I and pigeonpea in crop II and of a 9-year-old system with turmeric, a shade loving crop was studied at Pusa, Bihar in eastern India. Energy fixation, storage, net allocation in agronomic yield and energy released and exit from the 9-year-old system was 1.53, 4.30, 0.43 and 3.37 times in crop I and 1.67, 4.60, 0.53 and 3.30 times in crop II of the 3-year-old agroforestry system. The energy conservation efficiency in the 9-year-old system was higher (1.91%) as compared to crop I (1.24%) and crop II (1.15%) of the 3-year-old agroforestry system. The energy accumulation ratio in the 9-year-old system was 2.82 and 2.77 times higher in crop I and crop II, respectively, of the 3-year-old agroforestry system. The 3-year-old agroforestry system showed lower energy accumulation ratio resulting from less energy accumulation in perennial turnover in the form of leaf of tree and agricultural crops. The crop II system of the 3-year-old poplar agroforestry was more efficient system of management due to higher quanta of energy and higher cash return but one has to opt for shade loving intercrop turmeric with increase in age of the poplar plantation and more canopy closure. (Author)

  7. Plant Protease Inhibitors in Therapeutics-Focus on Cancer Therapy

    Science.gov (United States)

    Srikanth, Sandhya; Chen, Zhong

    2016-01-01

    Plants are known to have many secondary metabolites and phytochemical compounds which are highly explored at biochemical and molecular genetics level and exploited enormously in the human health care sector. However, there are other less explored small molecular weight proteins, which inhibit proteases/proteinases. Plants are good sources of protease inhibitors (PIs) which protect them against diseases, insects, pests, and herbivores. In the past, proteinaceous PIs were considered primarily as protein-degrading enzymes. Nevertheless, this view has significantly changed and PIs are now treated as very important signaling molecules in many biological activities such as inflammation, apoptosis, blood clotting and hormone processing. In recent years, PIs have been examined extensively as therapeutic agents, primarily to deal with various human cancers. Interestingly, many plant-based PIs are also found to be effective against cardiovascular diseases, osteoporosis, inflammatory diseases and neurological disorders. Several plant PIs are under further evaluation in in vitro clinical trials. Among all types of PIs, Bowman-Birk inhibitors (BBI) have been studied extensively in the treatment of many diseases, especially in the field of cancer prevention. So far, crops such as beans, potatoes, barley, squash, millet, wheat, buckwheat, groundnut, chickpea, pigeonpea, corn, and pineapple have been identified as good sources of PIs. The PI content of such foods has a significant influence on human health disorders, particularly in the regions where people mostly depend on these kind of foods. These natural PIs vary in concentration, protease specificity, heat stability, and sometimes several PIs may be present in the same species or tissue. However, it is important to carry out individual studies to identify the potential effects of each PI on human health. PIs in plants make them incredible sources to determine novel PIs with specific pharmacological and therapeutic effects due

  8. Genomic survey, gene expression analysis and structural modeling suggest diverse roles of DNA methyltransferases in legumes.

    Science.gov (United States)

    Garg, Rohini; Kumari, Romika; Tiwari, Sneha; Goyal, Shweta

    2014-01-01

    DNA methylation plays a crucial role in development through inheritable gene silencing. Plants possess three types of DNA methyltransferases (MTases), namely Methyltransferase (MET), Chromomethylase (CMT) and Domains Rearranged Methyltransferase (DRM), which maintain methylation at CG, CHG and CHH sites. DNA MTases have not been studied in legumes so far. Here, we report the identification and analysis of putative DNA MTases in five legumes, including chickpea, soybean, pigeonpea, Medicago and Lotus. MTases in legumes could be classified in known MET, CMT, DRM and DNA nucleotide methyltransferases (DNMT2) subfamilies based on their domain organization. First three MTases represent DNA MTases, whereas DNMT2 represents a transfer RNA (tRNA) MTase. Structural comparison of all the MTases in plants with known MTases in mammalian and plant systems have been reported to assign structural features in context of biological functions of these proteins. The structure analysis clearly specified regions crucial for protein-protein interactions and regions important for nucleosome binding in various domains of CMT and MET proteins. In addition, structural model of DRM suggested that circular permutation of motifs does not have any effect on overall structure of DNA methyltransferase domain. These results provide valuable insights into role of various domains in molecular recognition and should facilitate mechanistic understanding of their function in mediating specific methylation patterns. Further, the comprehensive gene expression analyses of MTases in legumes provided evidence of their role in various developmental processes throughout the plant life cycle and response to various abiotic stresses. Overall, our study will be very helpful in establishing the specific functions of DNA MTases in legumes.

  9. Mini core germplasm collections for infusing genetic diversity in plant breeding programs

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    Hari D Upadhyaya*, Devvart Yadav, Naresh Dronavalli, CLL Gowda, and Sube Singh

    2010-07-01

    Full Text Available Plant genetic resources are essential components to meet future food security needs of world. Crop germplasm diversitycontributes to developing improved crop cultivars aimed at increasing crop productivity. The large size of germplasmcollections, coupled with unavailability of detailed data and information, has resulted in low use (<1% of germplasmleading to a narrow genetic base in many crops. The miniaturization of crop collections with almost full representation ofgenetic diversity in the form of mini core (~1% of the entire collection approach is an effective methodology to enrichand enhance crop improvement programs. The concept and process of developing mini core at The International CropsResearch Institute for the Semi-Arid Tropics (ICRISAT has been recognized worldwide as an “International PublicGood” (IPG. The mini core provides a means for accessing the larger collections for further exploration and also helps inproper assessment of genetic diversity and population structure and for association mapping and targeted gene mining.Use of mini core approach will lead to greater utilization of diverse germplasm for developing broad-based cultivars,especially in the context of climate change. Many national programs have shown immense interest in evaluating minicore as reflected by the supply of 114 sets of mini core of chickpea, groundnut, pigeonpea, sorghum, pearl millet, foxtailmillet and finger millet to researchers in 14 countries. Scientists have been able to identify new and diverse sources ofvariation for morpho-agronomic, quality, biotic, and abiotic stress resistance traits in various crops. The molecularcharacterization of the mini core will further enhance its use in plant breeding programs.

  10. Ecosystem evaluation of post sand mining land in Cimalaka, Sumedang

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    A.R.F. Sholihah

    2014-01-01

    Full Text Available This research was conducted to evaluate ecosystem function of post-sand mining land in northern side of Layapan, Cimalaka, Sumedang, West Java (S 6o 47’ 33.68” and E 107o 58’18.73”, 744 m above sea level. Microclimate and soil characteristics measurements were carried out to describe the physical and chemical characteristics of the land. Vegetation analysis was conducted with plotting method. Ecosystem Function Analysis, including Landscape Function Analysis (LFA has been used to analyze the function of landscape. The results showed that average light intensity, air temperature and humidity were 15.2x103 ± 7.3x103 lux, 29.1±1.02oC and 69.7±7.5%. High light intensity made the air temperature rouse higher than normal, which is between 17.1oC to 22oC. As for the soil, soil organic content was 4-11%, porosity 4.65-24.43%, macronutrient content was low and C/N ratio was high. The results showed that LFA value for land stability was 33.24%, water infiltration 37.2%, nutrient cycle rate 15.28%. Those numbers showed that land condition was poor. From the LFA data, it was also known that vegetations had the highest contribution for all LFA parameters. From vegetation analysis, herbs species were 67 while bushes only 9, which at least 40 species were invasive alien species. Species with highest Important Value (IV from herb was Cajanus scarabaeoides and from bush was Mimosa pigra. Both of them are members of Fabaceae. It was concluded that the soil of this post sand mining land was highly nutrient poor; critical and couldn’t perform the regulation, habitat and biomass production function of ecosystem.

  11. Proteomics and Metabolomics: two emerging areas for legume improvement

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    Abirami eRamalingam

    2015-12-01

    Full Text Available The crop legumes such as chickpea, common bean, cowpea, peanut, pigeonpea, soybean, etc. are important source of nutrition and contribute to a significant amount of biological nitrogen fixation (>20 million tons of fixed nitrogen in agriculture. However, the production of legumes is constrained due to abiotic and biotic stresses. It is therefore imperative to understand the molecular mechanisms of plant response to different stresses and identify key candidate genes regulating tolerance which can be deployed in breeding programs. The information obtained from transcriptomics has facilitated the identification of candidate genes for the given trait of interest and utilizing them in crop breeding programs to improve stress tolerance. However, the mechanisms of stress tolerance are complex due to the influence of multi-genes and post-transcriptional regulations. Furthermore, stress conditions greatly affect gene expression which in turn causes modifications in the composition of plant proteomes and metabolomes. Therefore, functional genomics involving various proteomics and metabolomics approaches have been obligatory for understanding plant stress tolerance. These approaches have also been found useful to unravel different pathways related to plant and seed development as well as symbiosis. Proteome and metabolome profiling using high-throughput based systems have been extensively applied in the model legume species Medicago truncatula and Lotus japonicus, as well as in the model crop legume, soybean, to examine stress signalling pathways, cellular and developmental processes and nodule symbiosis. Moreover, the availability of protein reference maps as well as proteomics and metabolomics databases greatly support research and understanding of various biological processes in legumes. Protein-protein interaction techniques, particularly the yeast two-hybrid system have been advantageous for studying symbiosis and stress signalling in legumes. In

  12. Laboratory Information Management Software for genotyping workflows: applications in high throughput crop genotyping

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    Prasanth VP

    2006-08-01

    Full Text Available Abstract Background With the advances in DNA sequencer-based technologies, it has become possible to automate several steps of the genotyping process leading to increased throughput. To efficiently handle the large amounts of genotypic data generated and help with quality control, there is a strong need for a software system that can help with the tracking of samples and capture and management of data at different steps of the process. Such systems, while serving to manage the workflow precisely, also encourage good laboratory practice by standardizing protocols, recording and annotating data from every step of the workflow. Results A laboratory information management system (LIMS has been designed and implemented at the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT that meets the requirements of a moderately high throughput molecular genotyping facility. The application is designed as modules and is simple to learn and use. The application leads the user through each step of the process from starting an experiment to the storing of output data from the genotype detection step with auto-binning of alleles; thus ensuring that every DNA sample is handled in an identical manner and all the necessary data are captured. The application keeps track of DNA samples and generated data. Data entry into the system is through the use of forms for file uploads. The LIMS provides functions to trace back to the electrophoresis gel files or sample source for any genotypic data and for repeating experiments. The LIMS is being presently used for the capture of high throughput SSR (simple-sequence repeat genotyping data from the legume (chickpea, groundnut and pigeonpea and cereal (sorghum and millets crops of importance in the semi-arid tropics. Conclusion A laboratory information management system is available that has been found useful in the management of microsatellite genotype data in a moderately high throughput genotyping

  13. Evaluación de abonos verdes en el sistema de producción maíz-leguminosas

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    Prager M. Martin

    1989-06-01

    Full Text Available En la primera parte del ensayo, se estudió el comportamiento del peso de nódulos, peso fresco y peso seco de tres leguminosas (Crotalaria, Canavalia y Guandul a utilizar como abonos verdes y su efecto sobre sistemas de producción maíz-leguminosas (maíz-caupí, maíz-soya, maíz-frijol. Las variables consideradas (rendimiento, rendimiento equivalente de maíz, ingreso neto y tasa de retorno marginal mostraron el efecto positivo de esta práctica sobre esta forma de producción obteniéndose mejor respuesta de el sistema maíz-caupí. De los abonos verdes la crotalaria presentó el mejor efecto sobre los sistemas de producción. En la segunda parte del trabajo, se utilizó el sistema de producción de mejor comportamiento introduciendo al factor abono verde algunas modalidades de tratamiento, entre ellas efecto residual, adición de una segunda incorporación de los tres abonos verdes y efecto residual más la adición de abono químico. La consideración de las mismas variables usadas en la primera parte mostro mejores valores por la segunda incorporación de abono verde y la adición de abono químico en su orden, el efecto residual de los abonos permite obtener rendimientos superiores a los alcanzados por el testigo.This research was divided in two parts. The first studies the behavior of three legumes (Crotalaria, Canavalia and Cajanus as green manure, measuring weight nodules, green weight matter and dry weight matter of them, so measured the effect on three production systems: Maize- cowpea, Maize- soybean and Maize-bean. The indicators (yield, equivalent maize yields, net income and marginal return rate showed positive effect of green manure on this production systems too maize-cowpea system showed the best yields; Crotalaria was ,the best green manure. In second experimental stage maize- cowpea system was evaluated, using the same indicators and introducing new treatments related to green manure: its residual effects, addicional

  14. 木豆豆荚螟为害特点及其综合防治技术%Damages caused by Etiella zinckenella to pigeon pea and its integrated control measures

    Institute of Scientific and Technical Information of China (English)

    张晓声

    2011-01-01

    to pigeonpea by Etiella zinckenella. The reasonable application of pesticide could reduce the damage and improve the yield of pigeon pea. [Suggestion]Cleaning up the fields thoroughly in winter may reduce the insect source next year. Keeping the soil porous and enough light penetration under canopy, avoiding multiple variety plantations, enhancing field management and removing variant seedlings timely, using insecticides at proper time of flowering and pod stages were suggested as measures to protect flower and fruits.

  15. N-P-K balance in a milk production system on a C. nlemfuensis grassland and a biomass bank of P. purpureum CT-115 clone

    Science.gov (United States)

    Crespo, G.; Rodriguez, I.; Martinez, O.

    2009-04-01

    In very intensive milk production systems in Europe and America with the use of high amounts of chemical fertilizers, the nutrient recycling models consider the losses by leaching and N volatilization, as well as the hydro physical characteristics of the soil affecting the performance of this element (10; 6). However, in more extensive milk production systems, low input agriculture forming the natural cycle occurring within each farm, is of vital importance to potentate nutrient recycling for a stable animal production. The objective is the determination of the values of N, P and K inputs and outputs in a dairy farm with a sward composed by 60% of C. nlemfuensis and 40% of P. purpureum CT-115, associated with legumes in 28% of the area and the balance of these nutrients in the system using the "Recycling" software proposed by Crespo et al (2007). The grassland covered an area of 53.4 ha, composed by C. nlemfuensis (60%), P. purpureum CT-115 (40%) and L. leucocephala and C. cajan legumes intercropped in 28% of the area. The dairy herd consisted of 114 cows, 35 replacement heifers and 24 calves. There was a milk yield of 100 000 litters and the animals consumed 825 t DM from pastures and 75.1 t DM from other supplementary feeds. Nutrients extracted by pastures, nutrients intake by animals from pastures, symbiotically N fixation by legumes and N, P and K determinations outside the system due to animal production were determined (3-11). Volatilized ammonia, nutrient input and litter accumulated in the paddocks were measured once each season of the year. In the whole system the balance indicates negative values of N, P and K. Out of the total amount of nutrients consumed, animals used only 16 kg N, 5 Kg P and 4 Kg K for milk production, LW gain and calf production, the remainder returned to the system through excretions. Hence, more than 90% of the N and K, and approximately 81% of the P consumed by the animals were recycled to the system through the excretions. These

  16. Tannins Dynamic of 15 Tropical Trees and Shrubs%15种热带乔灌木单宁含量动态分析

    Institute of Scientific and Technical Information of China (English)

    李茂; 字学娟; 周汉林; 徐铁山; 刘国道

    2011-01-01

    Tannin contents of 15 tropical trees and shrubs were observed from March 2009 to Feb. 2010. Tannin levels of forage plants were ranked as: Ficus hispida L. F. (0. 39%), Erythrina variegata Linn. (0.77%); Cratylia argentea (Desv. ) Kuntze (0.78%); Dendrolobium triangulate (Retz. ) Schindl. (1. 16%); Cajanuscajan (Linn. ) Huth (1. 29%); Senna bicapsularis (Linn. ) Roxb. (1. 38%); Microcos paniculata Linn. (1.56%); Codariocalyx gyroides (Roxb. Ex Link) Hassk. (1.57%); Ficus auriculata Lour. (1. 62%); Senna didymobotrya (Fresen. ) H. S. Irwin et Barneby (1. 82%); Flemingia macro-phylla (Willd. ) Prain (1. 91%); Cratoxylum cochinchinense (Lour. ) Blume (2. 14%); Leucaena leuco-cephala (Lam.) de Wit (2.34%); Acacia fame siana (Linn.) Willd. (2.67%); Flueggea virosa (Roxb. Ex Willd.) Voigt (2. 80%). There were significant monthly dynamic of tannin contents in all tested tropical trees and shrubs leaves, related to species, growing period and environment. Additionally, utilization and tannin physiology were also discussed in this paper.%通过对15种热带乔灌木不同月份(2009年3月至2010年2月)单宁含量的测定,以期明确部分木本饲用植物单宁含量及其动态变化趋势.结果表明:15种热带乔灌木单宁平均含量由低到高顺序如下:对叶榕(Ficus hispida L.f.)(0.39%)<刺桐(Erythrina variegata Linn.)(0.77%)<克拉豆(Cratylia argentea( Desv.)Kuntze) (0.78%)<假木豆(Dendrolobium triangulare (Retz.) Schindl.)(1.16%)<木豆(Cajanus ca jan (Linn.)Huth)(1.29%)<双荚决明(Senna bicapsularis (Linn.) Roxb.)(1.38%)<破布叶(Microcos paniculata Linn.)(1.56%)<圆叶舞草(Codariocalyx gyroides (Roxb.ex Link) Hassk.)(1.57%)<大果榕(Ficus auriculata Lour.)(1.62%)<长穗决明(Senna did ymobotr ya( Fresen.)H.S.Irwin et Barneby)(1.82%)<大叶千斤拔(Flemingia macrophylla (Willd.) Prain)( 1.91%)<黄牛木(Cratoxylum cochinchinense (Lour.) Blume) (2.14%)<银合欢(Leucaena leucocephala( Lam

  17. DISCURSO DE POSESIÓN COMO PRESIDENTE DE LA ACADEMIA NACIONAL DE MEDICINA PERIODO 2016 A 2018

    Directory of Open Access Journals (Sweden)

    Herman Esguerra Villamizar

    2016-04-01

    Full Text Available

    En verdad hoy me acompañan un gran contento, una enorme satisfacción y un ánimo del espíritu increíble. Este hecho, el de posesionarme como nuevo presidente de la Academia Nacional de Medicina, es quizá uno de los honores más grandes que como médico he recibido en mi vida profesional y de académico. Hay un gran deseo en mí de lograr objetivos, cumplir responsabilidades y realizar servicios. Ustedes honorables académicos, han creído en mí y han depositado en mi persona el liderazgo y conducción de nuestra academia para los próximos dos años. Muchas gracias por ese honor que me confieren.

    Siempre hubo en mí, desde el estudiante de medicina, luego como médico rural, médico general, médico especialista en Medicina Interna y médico oncólogo clínico y de trasplante de médula ósea, el propósito de ser un médico “superior” (aún no sabría decir qué significado tiene este adjetivo. Pero aún a este tiempo, día a día siento la necesidad de planear, proyectar, realizar y servir como médico a mis pacientes, a mi comunidad, a las instituciones en que me muevo, a mi academia y a mi país.

    Inicié mi recorrido como médico oncólogo clínico en el Instituto Nacional de Cancerología, allí promoví y participé en la creación, organización y desarrollo de la especialidad de Oncología Clínica.

    Me adentré en el concepto de la integralidad en el manejo del paciente con cáncer y en la necesidad de educación médica en cáncer. Fundé servicios de Oncología en el Hospital Central de la Policía Nacional y en la Clínica Santa Rosa, de Cajanal, el primer servicio de carácter privado de Oncología en Bogotá hace 30 años: El Servicio de Oncología de la Clínica de Marly. Luego Radioterapia Oncología Marly S.A – Instituto de Oncología y la Asociación Colombiana de Enfermos de Cáncer. También participé en la creación del Banco Nacional de Medicamentos para el Cáncer y el primer servicio y

  18. En la Encrucijada del Ejercicio Profesional, la Administración y la Política. “Un testimonio de vida”.

    Directory of Open Access Journals (Sweden)

    María Teresa Forero de Saade

    2003-10-01

    ; humanismo era la condición sine qua non para establecer relaciones médico-paciente, compromiso con la comunidad y de ella los grupos vulnerables los prioritarios, cumplimiento de nuestra obligación de servir aún a costa de la seguridad personal o de las conveniencias sociales, económicas o de cualquier otra índole, en fin, entrega total y absoluta a esa bella profesión que escogimos libremente la medicina, para constituirnos en instrumentos de Dios, quien es en últimas el ÚNICO dueño de la vida y de la muerte.

    No soy yo quien pueda juzgar los hechos, pero me temo que los tiempos han cambiado y también los paradigmas que fueron nuestros faros. Por fortuna con muy honrosas excepciones he visto como al calor de las reformas hemos ido perdiendo el protagonismo que otrora se constituyó en el orgullo del ejercicio profesional cuya meta principal, por no decir que única, eran nuestros pacientes, para quienes fuimos consejeros, amigos, orientadores, además de sanadores de las enfermedades del cuerpo y muy especialmente de las del alma.

    Pasamos desapercibidos los cambios, que se iniciaron por allá en la la década del 60, del siglo pasado, cuando la medicina se socializó y de ser una prestación individual que el Médico ofrecía en la intimidad de su consultorio, pasó a ser la prestación de servicios institucionalizados por las Entidades a cargo de la seguridad social: ISS, Cajanal y las Cajas de Compensación Familiar, amén de las cajas departamentales y municipales de previsión que desde esa época pulularon por todo el territorio nacional.

    Sumergidos como era la costumbre, en la ciencia, la tecnología, la clínica y en el ejercicio puro de las especialidades, nos parecía un tanto deleznable el accionar de algunos colegas que impelidos por las circunstancias originadas en los cambios, iniciaron actividades en la administración de servicios de salud y de seguridad social, adelantándose a la propia academia que sólo años más tarde inici