WorldWideScience

Sample records for pig production systems

  1. Consumer attitudes to different pig production systems

    DEFF Research Database (Denmark)

    de Barcellos, Marcia Dutra; Grunert, Klaus G; Zhou, Yanfeng

    2013-01-01

    In many Western countries, consumers have shown an increasing interest to the way in which food products are being produced. This study investigates Chinese consumers' attitudes towards different pig production systems by means of a conjoint analysis. While there has been a range of studies...... on Western consumers' attitudes to various forms of food production, little is known about the level of Chinese consumers' attitudes. A cross-sectional survey was carried out with 472 participants in six Chinese cities. Results indicate that Chinese consumers prefer industrial pig production systems, where...... traditional pig breeds are raised, over large-scale and small family farms. Farms with maximum attention to food safety which furthermore can provide lean meat with consistent quality are also preferred. Imported pig breeds and tasty, but variable meat were rejected. A 3-cluster solution found that consumers...

  2. Energy and nutrient cycling in pig production systems

    Science.gov (United States)

    Lammers, Peter J.

    United States pig production is centered in Iowa and is a major influence on the economic and ecological condition of that community. A pig production system includes buildings, equipment, production of feed ingredients, feed processing, and nutrient management. Although feed is the largest single input into a pig production system, nearly 30% of the non-solar energy use of a conventional--mechanically ventilated buildings with liquid manure handling--pig production system is associated with constructing and operating the pig facility. Using bedded hoop barns for gestating sows and grow-finish pigs reduces construction resource use and construction costs of pig production systems. The hoop based systems also requires approximately 40% less non-solar energy to operate as the conventional system although hoop barn-based systems may require more feed. The total non-solar energy input associated with one 136 kg pig produced in a conventional farrow-to-finish system in Iowa and fed a typical corn-soybean meal diet that includes synthetic lysine and exogenous phytase is 967.9 MJ. Consuming the non-solar energy results in emissions of 79.8 kg CO2 equivalents. Alternatively producing the same pig in a system using bedded hoop barns for gestating sows and grow-finish pigs requires 939.8 MJ/pig and results in emission of 70.2 kg CO2 equivalents, a reduction of 3 and 12% respectively. Hoop barn-based swine production systems can be managed to use similar or less resources than conventional confinement systems. As we strive to optimally allocate non-solar energy reserves and limited resources, support for examining and improving alternative systems is warranted.

  3. HACCP system in radiation-hygiene control of pig production

    Directory of Open Access Journals (Sweden)

    Mitrović Radosav

    2005-01-01

    Full Text Available The paper presents instructive methodology for introducing and realizing the HACCP system in pig production, as a typical representative of intensive breeding, with the objective of establishing radiation-hygiene supervision. Attention was focused on the type of pig diet as the key link in the production chain, in fact the selection of the raw material components necessary in the production of pig feed concentrates for intense breeding conditions, and on the establishment of a certain radiation-hygiene balance through prognostic-selective methodology, as a guarantee of radiation safety.

  4. Chinese consumers' attitude towards different pig production systems

    DEFF Research Database (Denmark)

    de Barcellos, Marcia Dutra; Grunert, Klaus G.; Yanfeng, Z.

    2008-01-01

    This study investigates Chinese consumers' attitude towards different pig production systems by means of a conjoint analysis. While there has been a range of studies on western consumers' attitudes to various forms of food production, little is known about such attitudes in other cultural contexts...... to food safety which furthermore can provide lean meat with consistent quality are also preferred compared to farms that have less focus on food safety. Chinese consumers also rejected imported pig breeds and tasty but variable meat....

  5. Chinese consumers' attitude towards different pig production systems

    DEFF Research Database (Denmark)

    de Barcellos, Marcia Dutra; Grunert, Klaus G.; Yanfeng, Z.;

    2008-01-01

    This study investigates Chinese consumers' attitude towards different pig production systems by means of a conjoint analysis. While there has been a range of studies on western consumers' attitudes to various forms of food production, little is known about such attitudes in other cultural context...... to food safety which furthermore can provide lean meat with consistent quality are also preferred compared to farms that have less focus on food safety. Chinese consumers also rejected imported pig breeds and tasty but variable meat....

  6. Attitudes of Brazilian citizens towards pig production systems

    DEFF Research Database (Denmark)

    de Barcellos, Marcia Dutra; Kügler, Jens Oliver; Saab, Maria Stella Melo

    to identify Brazilian citizen clusters. Respondents' socio-demographic profile, attitudes towards issues that are expected to influence the way how people evaluate pig meat production systems, and consumption frequency of various pork products were used as background information for profiling. Three clusters...

  7. Attitudes of Brazilian citizens towards pig production systems

    DEFF Research Database (Denmark)

    de Barcellos, Marcia Dutra; Kügler, Jens Oliver; Saab, Maria Stella Melo

    The objective of this study is to map Brazilian citizen attitudes towards pig meat production systems and to investigate whether these attitudes associate with pork and pork product consumption. A conjoint experiment was carried out with empirical data collected from 475 respondents interviewed...... in southern and central-western states of Brazil. The experiment was based on the following pig farming characteristics: farm size, floor type, efforts to protect soil, air and water, fat content, and pork quality. The results of the conjoint analysis were used for a subsequent cluster analysis in order...... were identified as "average", "environmental conscious" and "tradition and animal welfare-oriented" citizens. Although attitudes towards environment and nature were indeed related to citizens' specific attitudes towards pig farming at the cluster level, the relationship between citizenship...

  8. Attitudes of European citizens towards pig production systems

    DEFF Research Database (Denmark)

    Krystallis, Athanasios; de Barcellos, Marcia Dutra; Kügler, Jens Oliver

    2009-01-01

    pork with specific fat content, and finally the preferred quality characteristics of the pork end-product. The results of the conjoint analysis were used for a subsequent cluster analysis in order to identify European citizen clusters. Respondents' socio-demographic profile, attitudes towards issues...... that are expected to influence the way people evaluate pig meat production systems, and consumption frequency of various pork products are used as background information to identify segment profiles. Pork appears to play a substantial role as a part of the diet for most respondents in the four countries...

  9. Multidirectional analysis of technical efficiency for pig production systems

    DEFF Research Database (Denmark)

    Labajavo, Katarina; Hansson, Helena; Asmild, Mette;

    2016-01-01

    Declining profitability and ongoing structural changes in the pig sector require thorough efficiency analysis of individual production factors. In this study we calculated technical efficiency indices for each input and output using multidirectional efficiency analysis and examined the relationsh...

  10. Attitudes of European citizens towards pig production systems

    DEFF Research Database (Denmark)

    Krystallis, Athanasios; de Barcellos, Marcia Dutra; Kügler, Jens Oliver

    2009-01-01

    of this study. The results of the conjoint experiment at the sample level show that people assigned most importance to animal and environmental well-being as criteria to discriminate between "good" and "bad" pig production practices, despite the fact that their attitudes towards environmental protection, animal...... and the need for an environment-friendly food production were indeed related to citizens' specific attitudes towards pig farming at the cluster level. However, the relationship between citizenship and consumption behaviour was found to be weak. What people think in their role as citizens related to pig...... welfare and industrial food production were only moderately strong. Moreover, the results of the cluster analysis allowed small-sized, clear-cut clusters of citizens to appear, which pay attention to specific pig farming attributes. In conclusion, attitudes towards environment and nature, animal welfare...

  11. Toxoplasma gondii Infection in Animal-Friendly Pig Production Systems

    NARCIS (Netherlands)

    Kijlstra, A.; Eissen, O.A.; Cornelissen, J.B.W.J.; Munniksma, K.; Eijck, I.A.J.M.; Kortbeek, T.

    2004-01-01

    PURPOSE. Consumption of undercooked pork meat products has been considered a major risk factor for contracting toxoplasmosis in humans. Indoor farming and improved hygiene have drastically reduced Toxoplasma infections in pigs over the past decades. Whether introduction of animal-friendly production

  12. Modelling the distribution of pig production and diseases in Thailand

    OpenAIRE

    Thanapongtharm, Weerapong

    2015-01-01

    This thesis, entitled “Modelling the distribution of pig production and diseases in Thailand”, presents many aspects of pig production in Thailand including the characteristics of pig farming system, distribution of pig population and pig farms, spatio-temporal distribution and risk of most important diseases in pig at present, and the suitability area for pig farming. Spatial distribution and characteristics of pig farming in Thailand were studied using time-series pig population data to des...

  13. Scales of renewability exemplified by a case study of three Danish pig production systems

    DEFF Research Database (Denmark)

    Wright, Christina; Østergård, Hanne

    2015-01-01

    (C)) exemplifies the use of this set of indicators. The results show that at the on-site scale the pig production systems had about the same fraction of renewable inputs of less than 0.5%. However, when the renewability fraction of inputs was accounted for at the global scale, the two organic systems...

  14. Prediction of Pig Trade Movements in Different European Production Systems Using Exponential Random Graph Models.

    Science.gov (United States)

    Relun, Anne; Grosbois, Vladimir; Alexandrov, Tsviatko; Sánchez-Vizcaíno, Jose M; Waret-Szkuta, Agnes; Molia, Sophie; Etter, Eric Marcel Charles; Martínez-López, Beatriz

    2017-01-01

    In most European countries, data regarding movements of live animals are routinely collected and can greatly aid predictive epidemic modeling. However, the use of complete movements' dataset to conduct policy-relevant predictions has been so far limited by the massive amount of data that have to be processed (e.g., in intensive commercial systems) or the restricted availability of timely and updated records on animal movements (e.g., in areas where small-scale or extensive production is predominant). The aim of this study was to use exponential random graph models (ERGMs) to reproduce, understand, and predict pig trade networks in different European production systems. Three trade networks were built by aggregating movements of pig batches among premises (farms and trade operators) over 2011 in Bulgaria, Extremadura (Spain), and Côtes-d'Armor (France), where small-scale, extensive, and intensive pig production are predominant, respectively. Three ERGMs were fitted to each network with various demographic and geographic attributes of the nodes as well as six internal network configurations. Several statistical and graphical diagnostic methods were applied to assess the goodness of fit of the models. For all systems, both exogenous (attribute-based) and endogenous (network-based) processes appeared to govern the structure of pig trade network, and neither alone were capable of capturing all aspects of the network structure. Geographic mixing patterns strongly structured pig trade organization in the small-scale production system, whereas belonging to the same company or keeping pigs in the same housing system appeared to be key drivers of pig trade, in intensive and extensive production systems, respectively. Heterogeneous mixing between types of production also explained a part of network structure, whichever production system considered. Limited information is thus needed to capture most of the global structure of pig trade networks. Such findings will be useful

  15. Environmental trade-offs of pig production systems under varied operational efficiencies

    DEFF Research Database (Denmark)

    McAuliffe, G. A.; Takahashi, T; Mogensen, Lisbeth

    2017-01-01

    Production of pork, the most consumed meat globally, is estimated to emit 668 m tonnes CO2-eq of greenhouse gases each year. Amongst various production systems that comprise the pig industry, grain-based intensive production is widely regarded as the largest polluter of the environment, and thus...... it is imperative to develop alternative systems that can provide the right balance between sustainability and food security. Using an original dataset from the Republic of Ireland, this paper examines the life-cycle environmental impacts of representative pig farms operating under varying production efficiencies....... For the baseline farm with an average production efficiency, global warming potential (GWP), acidification potential (AP) and eutrophication potential (EP) per kg carcass weight departing the slaughterhouse were estimated to be 3.5 kg CO2-eq, 43.8 g SO2-eq and 32.1 g PO4-eq, respectively. For herds with a higher...

  16. Opportunities and challenges for smallholder pig production systems in a mountainous region of Xishuangbanna, Yunnan Province, China.

    Science.gov (United States)

    Riedel, Simon; Schiborra, Anne; Huelsebusch, Christian; Huanming, Mao; Schlecht, Eva

    2012-12-01

    China's small-scale pig keepers are the largest community of pork producers worldwide. About 56 % of the world's pigs originate from such systems, each producing 2-5 head per year. This study analyzes pig smallholders in Xishuangbanna, a prefecture of Yunnan Province. Categorical principal component analysis and two-step cluster analysis were used to identify three main production systems: livestock-corn-based (LB; 41 %), rubber based (RB; 39 %), and pig based (PB; 20 %) systems. RB farms earn high income from rubber and fatten cross-bred pigs, often using purchased feeds. PB farms own similar-sized rubber plantations and raise pigs, with fodder mainly being cultivated and collected in the forest. LB farms grow corn, rice, and tea while also raising pigs, fed with collected and cultivated fodder as well. About one third of pigs were marketed (LB, 20 %; RB, 42 %; PB, 25 %), and local pig meat is highly appreciated in the nearby town. High mortality, low reproductive performance, and widespread malnourishment are the systems' main constraints. Basic training in hygiene and reproduction management could significantly increase production; most effective measures would be counterbalancing seasonal malnourishment and exploration of locally available protein feeds. Through support by external expertise, farmers could more effectively trade their pigs at lucrative town markets.

  17. Productivity and parasitic infections of pigs kept under different management systems by smallholder farmers in Mbeya and Mbozi districts, Tanzania.

    Science.gov (United States)

    Lipendele, Calvin Paul; Lekule, Faustine Paul; Mushi, Daniel Elias; Ngowi, Helena; Kimbi, Eliakunda Casmir; Mejer, Helena; Thamsborg, Stig Milan; Johansen, Maria Vang

    2015-08-01

    An on farm experiment was carried out to assess the effects of production systems on the performance of local pigs kept by smallholder farmers. Six villages from Mbeya and Mbozi districts, Tanzania were purposely selected based on the prominent pig production systems: free range, semi-confinement and total confinement. Fifteen pig keeping households were randomly selected from each village to participate in the study. A participatory rural appraisal and structured questionnaire were used for collecting information from the households on pig production and reproduction performance. In addition, a total of 180 weaner pigs, 2-3 months old, were purchased and randomly allocated to the 90 participating households. The pigs were subjected to three production systems: free range (M1), confinement with local diet (M2) and confinement with a compounded diet and anthelmintic treatment (M3). The anthelmintic treatment (piperazine citrate) was administered at 1 g per kg body weight. Faecal and blood samples were collected at month three of the experiment to assess the burden of intestinal helminths and sero-prevalence of Taenia solium cysticercosis, respectively. Sows kept under free range system were reported to have smaller litter size both at farrowing and at weaning compared to those kept under confinement. The experiment showed pigs under M3 had higher (P pigs in M2 (73 g/day) and M1 (68 g/day). In addition, pigs in M3 had higher body length and heart girth size with the feed to gain ratio of 8.5. Free range pigs tended to have lower faecal egg counts for most worm species compared to permanently confined pigs. Sero-prevalence of Taenia solium cysticercosis was 26%, with village prevalence ranging from 8 to 52%. Although pigs kept in M3 performed better than the rest, the compounded feed was too expensive for the farmers to afford. Locally available feed types combined with vitamin and mineral supplements may be a more sustainable option.

  18. Impact of organic pig production systems on CO2 emission, C sequestration and nitrate pollution

    DEFF Research Database (Denmark)

    Halberg, Niels; Hermansen, John Erik; Kristensen, Ib Sillebak

    2010-01-01

    compares the environmental impact of the main organic pig systems in Denmark. Normally, sows are kept in huts on grassland and finishing pigs are raised in stables with access to an outdoor run. One alternative practice is also rearing the fattening pigs on grassland all year round. The third method...

  19. Incorporating Risks in Economic Values for Pigs in Smallholder Production Systems in Kenya

    DEFF Research Database (Denmark)

    Mbuthias, Jackson; Rewe, Thomas; Okeno, Tobias;

    production systems in Kenya. In addition to traditional bio-economic profit model, a risk-rated model was used to derive risk-rated economic values. This model accounted for imperfect knowledge concerning risk attitude of farmers and variance of input and output prices. Positive economic values obtained......This study derived economic values for production traits (dressing percentage, live weight for growers and sow) and functional traits (feed intake for growers and sow, preweaning, postweaning, and sow survival rates, total number of piglets born, and farrowing interval) in different smallholder pig...... for traits DP, LWg, LWs, PoSR, PrSR, SoSR and TNB indicate that targeting them in improvement would positively impact profitability in pig breeding programmes. Accounting for risks in the EVs did not yield errors greater than ±50%. Therefore both traditional and risk-rated models can be satisfactorily used...

  20. Faecal Salmonella shedding in fattening pigs in relation to the presence of Salmonella antibodies in three pig production systems

    DEFF Research Database (Denmark)

    Bonde, Marianne Kjær; Sørensen, Jan Tind

    2012-01-01

    Human salmonellosis originating from pork is an important zoonotic disease, and the production of outdoor pigs may increase the risk of contaminating the food chain with Salmonella from environmental sources. The prevalence of faecal Salmonella shedding has therefore been compared in organic...

  1. Incorporating Risks in Economic Values for Pigs in Smallholder Production Systems in Kenya

    DEFF Research Database (Denmark)

    Mbuthias, Jackson; Rewe, Thomas; Okeno, Tobias

    production systems in Kenya. In addition to traditional bio-economic profit model, a risk-rated model was used to derive risk-rated economic values. This model accounted for imperfect knowledge concerning risk attitude of farmers and variance of input and output prices. Positive economic values obtained......This study derived economic values for production traits (dressing percentage, live weight for growers and sow) and functional traits (feed intake for growers and sow, preweaning, postweaning, and sow survival rates, total number of piglets born, and farrowing interval) in different smallholder pig...

  2. Smallholder pig production

    DEFF Research Database (Denmark)

    Braae, Uffe Christian; Ngowi, Helena; Johansen, Maria Vang

    2013-01-01

    -ranging of pigs and presence of neighbouring pigs were also identified as risk factors for the presence of lice. Three species of fleas were identified; Tunga penetrans, Echidnophaga gallinacea and Ctenocephalides canis. The prevalence of fleas was 5% and 13% within confined and free-range, respectively. Two pigs...

  3. Participatory assessment of animal health and husbandry practices in smallholder pig production systems in three high poverty districts in Uganda.

    Science.gov (United States)

    Dione, Michel M; Ouma, Emily A; Roesel, Kristina; Kungu, Joseph; Lule, Peter; Pezo, Danilo

    2014-12-01

    While animal health constraints have been identified as a major limiting factor in smallholder pig production in Uganda, researchers and policy makers lack information on the relative incidence of diseases and their impacts on pig production. This study aimed to assess animal health and management practices, constraints and opportunities for intervention in smallholder pig value chains in three high poverty districts of Uganda. Semi-qualitative interview checklists through Focus Group Discussions (FGDs) were administered to 340 pig farmers in 35 villages in Masaka, Kamuli and Mukono districts. Quantitative data was obtained during the exercise through group consensus. Results of FGDs were further triangulated with secondary data and information obtained from key informant interviews. Findings show that pig keeping systems are dominated by tethering and scavenging in rural areas. In peri-urban and urban areas, intensive production systems are more practiced, with pigs confined in pens. The main constraints identified by farmers include high disease burden such as African swine fever (ASF) and parasites, poor housing and feeding practices, poor veterinary services, ineffective drugs and a general lack of knowledge on piggery management. According to farmers, ASF is the primary cause of pig mortality with epidemics occurring mainly during the dry season. Worms and ectoparasites namely; mange, lice and flies are endemic leading to stunted growth which reduces the market value of pigs. Diarrhoea and malnutrition are common in piglets. Ninety-three percent of farmers say they practice deworming, 37% practice ectoparasite spraying and 77% castrate their boars. Indigenous curative treatments include the application of human urine and concoctions of local herbs for ASF control and use of old engine oil or tobacco extracts to control ectoparasites. There is a need for better technical services to assist farmers with these problems.

  4. Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System

    Directory of Open Access Journals (Sweden)

    Eulalia de la Torre

    2014-01-01

    Full Text Available Integrons are one of the genetic elements involved in the acquisition of antibiotic resistance. The aim of the present research is to investigate the presence of integrons in commensal Escherichia coli (E. coli strains, isolated from pigs at different stages of production system and from the environment in an Argentinian farm. Five sows postpartum and five randomly chosen piglets from each litter were sampled by rectal swabs. They were sampled again at day 21 and at day 70. Environmental samples from the farm were also obtained. E. coli containing any integron class or combination of both integrons was detected by polymerase chain reaction in 100% of sows and in piglets at different stages of production: farrowing pen stage 68.1%;, weaning 60%, and growing/finishing 85.8%, showing an increase along the production system. From environmental samples 78.4% of E. coli containing any integron class was detected. We conclude that animals and farm environment can act as reservoirs for potential spread of resistant bacteria by means of mobile genetic elements as integrons, which has a major impact on production of food animals and that can reach man through the food chain, constituting a problem for public health.

  5. Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System.

    Science.gov (United States)

    de la Torre, Eulalia; Colello, Rocío; Padola, Nora Lía; Etcheverría, Analía; Rodríguez, Edgardo; Amanto, Fabián; Tapia, María Ofelia; Soraci, Alejandro Luis

    2014-01-01

    Integrons are one of the genetic elements involved in the acquisition of antibiotic resistance. The aim of the present research is to investigate the presence of integrons in commensal Escherichia coli (E. coli) strains, isolated from pigs at different stages of production system and from the environment in an Argentinian farm. Five sows postpartum and five randomly chosen piglets from each litter were sampled by rectal swabs. They were sampled again at day 21 and at day 70. Environmental samples from the farm were also obtained. E. coli containing any integron class or combination of both integrons was detected by polymerase chain reaction in 100% of sows and in piglets at different stages of production: farrowing pen stage 68.1%;, weaning 60%, and growing/finishing 85.8%, showing an increase along the production system. From environmental samples 78.4% of E. coli containing any integron class was detected. We conclude that animals and farm environment can act as reservoirs for potential spread of resistant bacteria by means of mobile genetic elements as integrons, which has a major impact on production of food animals and that can reach man through the food chain, constituting a problem for public health.

  6. Studies on preventive strategies and alternative treatments against roundworm in organic pig production systems

    NARCIS (Netherlands)

    Borgsteede, F.H.M.; Gaasenbeek, C.P.H.; Krimpen, van M.M.; Maurer, V.; Mejer, H.E.; Spoolder, H.A.M.; Thamsborg, S.M.; Vermeer, H.M.

    2011-01-01

    Organic and many ‘low input’ production standards for pigs require outdoor access, and as a consequence expose the animals to a wide range of potential endo- and ectoparasite infection sources. Conventional anthelmintics are used to control parasitic infections, but their preventive use is banned by

  7. Incorporating Risks in Economic Values for Pigs in Smallholder Production Systems in Kenya

    DEFF Research Database (Denmark)

    Mbuthias, Jackson; Rewe, Thomas; Okeno, Tobias;

    This study derived economic values for production traits (dressing percentage, live weight for growers and sow) and functional traits (feed intake for growers and sow, preweaning, postweaning, and sow survival rates, total number of piglets born, and farrowing interval) in different smallholder pig...

  8. Production of pigs expressing a transgene under the control of a tetracycline-inducible system.

    Directory of Open Access Journals (Sweden)

    Yong-Xun Jin

    Full Text Available Pigs are anatomically and physiologically closer to humans than other laboratory animals. Transgenic (TG pigs are widely used as models of human diseases. The aim of this study was to produce pigs expressing a tetracycline (Tet-inducible transgene. The Tet-on system was first tested in infected donor cells. Porcine fetal fibroblasts were infected with a universal doxycycline-inducible vector containing the target gene enhanced green fluorescent protein (eGFP. At 1 day after treatment with 1 µg/ml doxycycline, the fluorescence intensity of these cells was increased. Somatic cell nuclear transfer (SCNT was then performed using these donor cells. The Tet-on system was then tested in the generated porcine SCNT-TG embryos. Of 4,951 porcine SCNT-TG embryos generated, 850 were cultured in the presence of 1 µg/ml doxycycline in vitro. All of these embryos expressed eGFP and 15 embryos developed to blastocyst stage. The remaining 4,101 embryos were transferred to thirty three surrogate pigs from which thirty eight cloned TG piglets were obtained. PCR analysis showed that the transgene was inserted into the genome of each of these piglets. Two TG fibroblast cell lines were established from these TG piglets, and these cells were used as donor cells for re-cloning. The re-cloned SCNT embryos expressed the eGFP transgene under the control of doxycycline. These data show that the expression of transgenes in cloned TG pigs can be regulated by the Tet-on/off systems.

  9. Improving efficiency in pig production

    African Journals Online (AJOL)

    important. The production of lean meat is shown to be improved significantly by adopting ... is interested in the biology of pig production, then biological efficiency and its .... scientific basis, I have calculated the economic effects of a number of ...

  10. Smallholder pig production systems along a periurban-rural gradient in the Western provinces of the Democratic Republic of the Congo

    Directory of Open Access Journals (Sweden)

    Bienvenu Kambashi

    2014-08-01

    Full Text Available In the Democratic Republic of the Congo (DRC, pigs are raised almost exclusively by smallholders either in periurban areas of major cities such as Kinshasa or in rural villages. Unfortunately, little information is available regarding pig production in the Western part of the DRC, wherefore a survey was carried out to characterize and compare 319 pig production systems in their management and feeding strategies, along a periurban - rural gradient inWestern provinces of the DRC. Pig breeding was the main source of income (43% and half of respondents were active in mixed pig and crop production, mainly vegetable garden. Depending on the location, smallholders owned on average 18 pigs, including four sows. Piglet mortality rate varied from 9.5 to 21.8% while average weaned age ranged between 2.2 and 2.8 months. The major causes of mortality reported by the farmers were African swine fever 98 %, swine erysipelas (60 %, erysipelas trypanosomiasis (31 %, swine worm infection (17 %, and diarrhoea (12 %. The majority of the pigs were reared in pens without free roaming and fed essentially with locally available by-products and forage plants whose nature varied according with the location of the farm. The pig production systems depended on the local environment; particularly in terms of workforces, herd structure and characteristics, production parameters, pig building materials, selling price and in feed resources. It can be concluded that an improvement of Congolese pig production systems should consider (1 a reduction of inbreeding, (2 an improvement in biosafety to reduce the incidence of African swine fever and the spread of other diseases, and (3 an improvement in feeding practices.

  11. Spatial and Functional Organization of Pig Trade in Different European Production Systems: Implications for Disease Prevention and Control.

    Science.gov (United States)

    Relun, Anne; Grosbois, Vladimir; Sánchez-Vizcaíno, José Manuel; Alexandrov, Tsviatko; Feliziani, Francesco; Waret-Szkuta, Agnès; Molia, Sophie; Etter, Eric Marcel Charles; Martínez-López, Beatriz

    2016-01-01

    Understanding the complexity of live pig trade organization is a key factor to predict and control major infectious diseases, such as classical swine fever (CSF) or African swine fever (ASF). Whereas the organization of pig trade has been described in several European countries with indoor commercial production systems, little information is available on this organization in other systems, such as outdoor or small-scale systems. The objective of this study was to describe and compare the spatial and functional organization of live pig trade in different European countries and different production systems. Data on premise characteristics and pig movements between premises were collected during 2011 from Bulgaria, France, Italy, and Spain, which swine industry is representative of most of the production systems in Europe (i.e., commercial vs. small-scale and outdoor vs. indoor). Trade communities were identified in each country using the Walktrap algorithm. Several descriptive and network metrics were generated at country and community levels. Pig trade organization showed heterogeneous spatial and functional organization. Trade communities mostly composed of indoor commercial premises were identified in western France, northern Italy, northern Spain, and north-western Bulgaria. They covered large distances, overlapped in space, demonstrated both scale-free and small-world properties, with a role of trade operators and multipliers as key premises. Trade communities involving outdoor commercial premises were identified in western Spain, south-western and central France. They were more spatially clustered, demonstrated scale-free properties, with multipliers as key premises. Small-scale communities involved the majority of premises in Bulgaria and in central and Southern Italy. They were spatially clustered and had scale-free properties, with key premises usually being commercial production premises. These results indicate that a disease might spread very differently

  12. Spatial and functional organization of pig trade in different european production systems: implications for disease prevention and control

    Directory of Open Access Journals (Sweden)

    Anne eRelun

    2016-02-01

    Full Text Available Understanding the complexity of live pig trade organization is a key factor to predict and control major infectious diseases such as Classical or African swine fever. Whereas the organization of pig trade has been described in several European countries with indoor commercial production systems, little information is available on this organisation in other systems like outdoor or small-scale systems. The objective of this study was to describe and compare the spatial and functional organisation of live pig trade in different European countries and different production systems. Data on premise characteristics and pig movements between premises were collected during 2011 from Bulgaria, France, Italy and Spain, which swine industry is representative of most of the production systems in Europe (i.e. commercial vs small-scale; outdoor vs indoor. Trade communities were identified in each country using the Walktrap algorithm. Several descriptive and network metrics were generated at country and community level. Pig trade organization showed heterogeneous spatial and functional organization. Trade communities mostly composed of indoor commercial premises were identified in Western France, Northern Italy, Northern Spain and North-Western Bulgaria. They covered large distances, overlapped in space, demonstrated both scale-free and small-world properties, with a role of trade operators and multipliers as key premises. Trade communities involving outdoor commercial premises were identified in Western Spain, South-Western and Central France. They were more spatially clustered, demonstrated scale-free properties, with multipliers as key premises. Small-scale communities involved the majority of premises in Bulgaria and in central and Southern Italy. They were spatially clustered and had scale-free properties, with key premises usually being commercial production premises. These results indicate that a disease might spread very differently according to the

  13. High frequency of porcine norovirus infection in finisher units of Brazilian pig-production systems.

    Science.gov (United States)

    Silva, Patrícia F N; Alfieri, Alice F; Barry, Aline F; de Arruda Leme, Raquel; Gardinali, Noemi R; van der Poel, Wim H M; Alfieri, Amauri Alcindo

    2015-01-01

    Norovirus (NoV) is a member of the Caliciviridae family and is considered an emerging human enteric pathogen. NoVs are detected in farm animals such as cattle, sheep and pigs. Porcine NoV (PoNoV) is widespread worldwide, but frequency of infection is often low. This study aimed to investigate the natural PoNoV infection from adult animals of an important Brazilian pig-production region. Faecal samples (n = 112) of asymptomatic pigs aged 9 to 24 weeks old were collected from 16 grower-to-finish herds located in Paraná state, Brazilian Southern region, and evaluated for PoNoV presence. A reverse transcription-polymerase chain reaction (RT-PCR) assay was performed using specific primers that target a conserved region of the virus capsid gene (VP1). PoNoV was detected in 58 (51.8%) of the 112 faecal samples and in 14 (87.5%) of the 16 herds evaluated. Six of the obtained amplicons were submitted to phylogenetic genotyping analysis. The higher nucleotide (86.5-97.4%) and amino acid (100%) similarities of the sequences in this study were with the representative strains of the porcine NoV genogroup II genotype 11 (PoNoV GII-11). These results reveal that PoNoV infection is endemic in one of the most important pork production areas of Brazil and that the PoNoV GII-11 is prevalent in this region.

  14. Animal health and welfare in production systems for organic fattening pigs

    DEFF Research Database (Denmark)

    Lindgren, Kristina; Bochicchio, Davide; Hegelund, Lene;

    2014-01-01

    respiratory problems, skin lesions (including abscesses and hernias) and tail wounds compared to conventional pigs. On the other hand, remarks because of joint lesions and white spot livers were more common among organic pigs. The risk of parasitic infections in organic fattening pigs has been confirmed...

  15. Longitudinal study of distributions of similar antimicrobial-resistant Salmonella serovars in pigs and their environment in two distinct swine production systems.

    Science.gov (United States)

    Keelara, Shivaramu; Scott, H Morgan; Morrow, William M; Gebreyes, Wondwossen A; Correa, Maria; Nayak, Rajesh; Stefanova, Rossina; Thakur, Siddhartha

    2013-09-01

    The aim of this longitudinal study was to determine and compare the prevalences and genotypic profiles of antimicrobial-resistant (AR) Salmonella isolates from pigs reared in antimicrobial-free (ABF) and conventional production systems at farm, at slaughter, and in their environment. We collected 2,889 pig fecal and 2,122 environmental (feed, water, soil, lagoon, truck, and floor swabs) samples from 10 conventional and eight ABF longitudinal cohorts at different stages of production (farrowing, nursery, finishing) and slaughter (postevisceration, postchill, and mesenteric lymph nodes [MLN]). In addition, we collected 1,363 carcass swabs and 205 lairage and truck samples at slaughter. A total of 1,090 Salmonella isolates were recovered from the samples; these were isolated with a significantly higher prevalence in conventionally reared pigs (4.0%; n = 66) and their environment (11.7%; n = 156) than in ABF pigs (0.2%; n = 2) and their environment (0.6%; n = 5) (P pigs on farms, while a higher prevalence was detected among the carcasses at slaughter. The dynamics of Salmonella prevalence in pigs and carcasses were reciprocated in the farm and slaughter environment, clearly indicating an exchange of this pathogen between the pigs and their surroundings. Furthermore, the phenotypic and genotypic fingerprint profile results underscore the potential role played by environmental factors in dissemination of AR Salmonella to pigs.

  16. Animal health and welfare in production systems for organic fattening pigs

    DEFF Research Database (Denmark)

    Lindgren, Kristina; Bochicchio, Davide; Hegelund, Lene

    2014-01-01

    respiratory problems, skin lesions (including abscesses and hernias) and tail wounds compared to conventional pigs. On the other hand, remarks because of joint lesions and white spot livers were more common among organic pigs. The risk of parasitic infections in organic fattening pigs has been confirmed....... To control endoparasites, outdoor areas should be rotated with as long interval as possible, i.e. by including the pigs in the crop rotation. Outdoor housing with functional wallows and access to grass and roots or outdoor runs and roughage can enhance pig welfare and reduce pen-mate-directed oral activity...

  17. Influence of amino acid level and production system on performance, health and behaviour in organic growing pigs

    OpenAIRE

    Høøk Presto, M.; Andersson, H.K.; Wallgren, P; LINDBERG, J.E.

    2008-01-01

    The influence of dietary amino acid levels (recommended, 7% and 14% lower) on performance and carcass quality was studied in organic indoor and outdoor pigs fed ad libitum in a 2-phase feeding system. The outdoor pigs grew faster during phase 2 than the indoor pigs (p=0.001), although feed conversion ratio did not differ (p=0.358). Dressing percentage was higher for outdoor than for indoor pigs (p=0.011) but lean meat content did not differ (p=0.904). The results indicate a discrepancy betwee...

  18. pig production in Zimbabwe

    African Journals Online (AJOL)

    stages of production to the limits dictated by the genetics of the animals. ... attempts are made to consider both the genetics and environment and to balance them. ... Breeding females were observed for oestrus and those in standing heat were ...

  19. Influence of production system in local and conventional pig breeds on stress indicators at slaughter, muscle and meat traits and pork eating quality.

    Science.gov (United States)

    Lebret, B; Ecolan, P; Bonhomme, N; Méteau, K; Prunier, A

    2015-08-01

    Sensory quality of pork is a complex phenotype determined by interactions between genetic and environmental factors. This study aimed at describing the respective influences of breed and production system on the development of pork quality. Plasma stress indicators and Longissimus muscle (LM) composition, physicochemical and sensory quality traits were determined in two contrasted breeds - the conventional Large White (LW, n=40) and the French local Basque (B, n=60). Pigs were reared in either a conventional (C; n=20 per breed), alternative (A; sawdust bedding and outdoor area, n=20 per breed) or extensive system (E; free-range, n=20 B). All the pigs from A and C systems were slaughtered at the same slaughterhouse, whereas B pigs from the E system were slaughtered at a local commercial abattoir. Major breed differences were found for almost all traits under study. LM from B pigs exhibited higher lipid, lower water and collagen concentrations, as well as lower collagen thermal solubility (P0.05) influence plasma stress indicators, LM chemical composition and physicochemical or sensory traits of pork. In contrast, within the B pigs, the E system affected the meat quality more. Lower plasma cortisol levels (Pskin lesions (Pimpact the eating quality of pork. Altogether, this study demonstrates that differences in meat quality between B and LW breeds can be modulated by extensive pig production system.

  20. Weight and season affects androstenone and skatole occurrence in entire male pigs in organic pig production

    DEFF Research Database (Denmark)

    Thomsen, Rikke; Edwards, Sandra; Jensen, Bent Borg

    2015-01-01

    was found between seasons. The study concludes that decreasing live weight at slaughter could be an applicable management tool to reduce risk of boar taint and the level of tainted carcasses for a future production of entire male pigs within the organic pig production system, although further studies......To investigate the extent to which the level of androstenone and skatole decreases with a decrease in live weight and/or age at slaughter of entire male pigs produced under organic standards, 1174 entire male pigs were raised in parallel in five organic herds, distributed across four batches...... in summer and winter. The median androstenone level was high for organic entire male pigs (1.9 μg/g), but varied greatly both within and between herds. Median skatole level was 0.05 μg/g, also with a wide range both within and between herds. Decreasing live weight over the range of 110 ± 15.6 kg s...

  1. Productivity and parasitic infections of pigs kept under different management systems by smallholder farmers in Mbeya and Mbozi districts, Tanzania

    DEFF Research Database (Denmark)

    Lipendele, Calvin Paul; Lekule, Faustine Paul; Mushi, Daniel Elias

    2015-01-01

    ). The anthelmintic treatment (piperazine citrate) was administered at 1 g per kg body weight. Faecal and blood samples were collected at month three of the experiment to assess the burden of intestinal helminths and sero-prevalence of Taenia solium cysticercosis, respectively. Sows kept under free range system were...... and heart girth size with the feed to gain ratio of 8.5. Free range pigs tended to have lower faecal egg counts for most worm species compared to permanently confined pigs. Sero-prevalence of Taenia solium cysticercosis was 26%, with village prevalence ranging from 8 to 52%. Although pigs kept in M3...

  2. Pigging ends freezeups in caustic piping system

    Energy Technology Data Exchange (ETDEWEB)

    Gros, R.; Gaines, A.

    1985-03-01

    Convent Chemical Corporation in Convent, LA produces and ships bulk quantities of chlorine and caustic soda (NaOH). The caustic soda is available in various grades, including a 50% aqueous solution that freezes at 56/sup 0/F. An extensive network of chemical-resistant polypropylene-lined steel pipe (without heat tracing) is used to transfer the caustic soda from the production area to storage tanks and to the loading facilities for tank trucks, rail tank cars and barges. A sudden drop in ambient temperature can cause freezeup of the caustic transfer pipes which may result in downtime of as much as a week. Convent plant engineers designed a pigging system for the outdoor caustic transfer lines in the tank farm and to the loading stations. The patented design pig, (internal pipeline cleaner) consists of a flexible, bullet-shaped cylinder of chemical-resistant polyurethane foam with strips of urethane rubber on the surface, or with a solid coating of the tough material. Fluid or gas pressure on the sealed concave base propels the flexible pig through the pipe, valves, elbows, and other fittings, and material ahead of the pig is discharged into an appropriate receiver. The pigging system has eliminated the caustic freezing and plugging problems since it was installed in the summer of 1981. The flexible pig, propelled by 80 psi air, is used to clear the pipelines whenever caustic is transferred during the winter months. The air-propelled pig is designed and sized to easily pass through restrictions in the piping system, such as reduced port plug valves, but was once stuck when it reached a section of pipe that had collapsed. A pig containing the Cobalt 60 nuclear element was inserted into the line to quickly locate the stuck pig with the Geiger counter. The faulty section of pipe was replaced with a new polypropylene lined spool piece.

  3. Energy production, nutrient recovery and greenhouse gas emission Potentials from Integrated Pig Manure Management Systems

    DEFF Research Database (Denmark)

    Prapaspongsa, Trakarn; Poulsen, Tjalfe; Hansen, Jens Aage

    2010-01-01

    of waste materials were considered. Data for the analyses were obtained from existing waste treatment facilities, experimental plants, laboratory measurements and literature. The assessment reveals that incineration combined with liquid/solid separation and drying of the solids is a promising management......Improper management of pig manure has resulted in environmental problems such as surface water eutrophication, ground water pollution, and greenhouse gas emissions. This study develops and compares 14 alternative manure management scenarios aiming at energy and nutrient extraction. The scenarios...... based on combinations of thermal pretreatment, anaerobic digestion, anaerobic co-digestion, liquid/solid separation, drying, incineration, and thermal gasification were compared with respect to their energy, nutrient and greenhouse gas balances. Both sole pig manure and pig manure mixed with other types...

  4. Improved sustainability in organic pig production

    OpenAIRE

    Kongsted, Anne Grete; Hermansen, John E.; Jakobsen, Malene

    2015-01-01

    The local and global sale of organic pork produced in Denmark has increased markedly in recent years. Organic pig production is associated with several positive aspects from a societal point of view e.g. very low use of antibiotics and animals being able to express more of their natural behavior compared to conventional production. However, there are some challenges regarding sustainability that needs to be addressed. In Denmark, organic pig production is based on outdoor sow production all y...

  5. Description of the pig production systems, biosecurity practices and herd health providers in two provinces with high swine density in the Philippines.

    Science.gov (United States)

    Alawneh, J I; Barnes, T S; Parke, C; Lapuz, E; David, E; Basinang, V; Baluyut, A; Villar, E; Lopez, E L; Blackall, P J

    2014-05-01

    A cross-sectional study was conducted between October 2011 and March 2012 in two major pig producing provinces in the Philippines. Four hundred and seventy one pig farms slaughtering finisher pigs at government operated abattoirs participated in this study. The objectives of this study were to group: (a) smallholder (S) and commercial (C) production systems into patterns according to their herd health providers (HHPs), and obtain descriptive information about the grouped S and C production systems; and (b) identify key HHPs within each production system using social network analysis. On-farm veterinarians, private consultants, pharmaceutical company representatives, government veterinarians, livestock and agricultural technicians, and agricultural supply stores were found to be actively interacting with pig farmers. Four clusters were identified based on production system and their choice of HHPs. Differences in management and biosecurity practices were found between S and C clusters. Private HHPs provided a service to larger C and some larger S farms, and have little or no interaction with the other HHPs. Government HHPs provided herd health service mainly to S farms and small C farms. Agricultural supply stores were identified as a dominant solitary HHP and provided herd health services to the majority of farmers. Increased knowledge of the routine management and biosecurity practices of S and C farmers and the key HHPs that are likely to be associated with those practices would be of value as this information could be used to inform a risk-based approach to disease surveillance and control.

  6. Prevalence of porcine cysticercosis and associated risk factors in smallholder pig production systems in Mbeya region, southern highlands of Tanzania

    DEFF Research Database (Denmark)

    Komba, Erick V. G.; Kimbi, Eliakunda C.; Ngowi, Helena A.

    2013-01-01

    Porcine cysticercosis (PC) caused by the larval stage of a zoonotic tapeworm Taenia solium, is known to pose serious economic losses and public health risk among smallholder pig production communities. The present study was conducted to determine prevalence and associated risk factors for PC...

  7. Serological Prevalence of Enteropathogenic Yersinia spp. in Pigs and Wild Boars from Different Production Systems in the Moravian Region, Czech Republic.

    Science.gov (United States)

    Lorencova, Alena; Babak, Vladimir; Lamka, Jiri

    2016-05-01

    Human yersiniosis caused by pathogenic Yersinia spp. is one of the most common reported zoonoses in the European Union and pigs are considered as the major reservoir of these bacteria. Serological testing represents a suitable method to obtain information about the prevalence of enteropathogenic Yersinia spp. in food animals. The prevalence of antibodies against enteropathogenic Yersinia spp. was studied in 319 slaughtered pigs and 135 wild boars from different production systems in the Moravian region (Czech Republic) using a commercially available ELISA test (an apparent prevalence). The seroprevalence was significantly associated with the type of breeding system, with the lowest seroprevalence being observed in household-raised pigs (13/29, 44.8%). No significant difference between the prevalence of anti-Yersinia antibodies in conventional (146/180, 81.1%) and organic pigs (92/110, 83.6%) was found. Antibodies were found in 65.9% (89/135) of wild boars without a significant difference between adult (23/41, 56.1%) and young (66/94, 70.2%) animals. Seropositivity was significantly higher in domestic (251/319, 78.7% in total) compared to feral pigs. A Bayesian approach taking into account the sensitivity and specificity of the ELISA test was used to estimate the true prevalence of anti-Yersinia antibodies in pigs and wild boars. According to our results, domestic pigs and wild boars proved to be an important reservoir of enteropathogenic Yersinia in the Czech Republic. Attention should be paid to good hygienic practice during slaughtering and handling of meat to prevent meat contamination and subsequently human infection.

  8. Impacts of projected climate change on productivity and nitrogen leaching of crop rotations in arable and pig farming systems in Denmark

    DEFF Research Database (Denmark)

    Doltra, Jordi; Lægdsmand, Mette; Olesen, Jørgen E

    2014-01-01

    in Denmark, differing in soil and climate, and representative of the selected production systems. The CO2 effects were modelled using projected CO2 concentrations for the A1B emission scenario. Crop rotations were irrigated (sandy soil) and unirrigated (sandy loam soil), and all included systems...... rather than single crops for impact assessments. Potato and sugar beet in arable farming and grain maize in pig farming contributed most to the productivity increase in the future scenarios. The highest productivity was obtained in the arable system on the sandy loam soil, with an increase of 20...

  9. MODELING REGIONAL SYSTEMS OF BREEDING PIGS

    Directory of Open Access Journals (Sweden)

    Svinarev I. Y.

    2015-12-01

    Full Text Available The article contains the experience of the development of the methodology and the computer program for calculation of regional and local systems of pigs hybridization at the example of the Rostov region (Russia. Crossing the GP lines for F1 should be organized in multiplier farm, which may be separate farms and to be part of large commercial farms. For the production of F1 in a multiplier farm, we must breed a purebred specialized paternal and a maternal line, selected on the effect of combining ability. For the successful functioning of the system of hybridization, it is necessary to build a genetic pyramid, including breeding and genetic centers (nucleus farm, multiplier farm, reproducing the baseline. The article gives a detailed calculation of sow population of levels of P, GP, GGP for maternal and paternal breeds of pigs. The program uses user-defined parameters of pigs productivity, of the simulated population, and the parameters characterizing the intensity of selection of young animals. To ensure annual production of 1,822 million pigs in the Rostov region it is necessary to provide the availability of brood stock in the amount of 89 thousand heads, 6 800 heads in the structure of grandparent flocks (GP, 730 heads in the structure of the Grand-Grand-parent stock (GGP, excluding sows second maternal and paternal breeds

  10. Pre-weaning performance of the Zimbabwean Mukota and Large White × Mukota pigs under low-input production systems

    Directory of Open Access Journals (Sweden)

    M. Chimonyo,

    2011-06-01

    Full Text Available Data from 434 litters farrowed between January 1998 and August 2003 was used to evaluate the piglet performance of Mukota and Large White × Mukota piglets up to weaning at 35 days (± 4 days. The traits studied were litter size at 21 days of age, total weight of piglets at 21 days of age, body weight gain to 21 days, number of piglets weaned, weight at weaning, body weight gain to weaning, body weight gain from 21 to 35 days and preweaning mortality. The month of farrowing and breed of sire did not affect litter size at day 21. The overall body weight gain from birth to weaning was not influenced by the sex of the piglet. At advanced parities, Mukota piglets had higher (P<0.05 litter weights at weaning than crossbred piglets. Mortality rates increased (P<0.05 up to the 5thparity and then dropped to 8th parity. The highest mortality rates were observed for litters that were farrowed during the cold months. Based on litter weights at weaning, it was concluded that Mukota pigs could be suitable for both low and medium input production systems.

  11. Seasonal organic pig production with a local breed

    DEFF Research Database (Denmark)

    Kongsted, Anne Grete; Claudi-Magnussen, C.; Horsted, Klaus

    2009-01-01

    2007 and 12 gilts inMay 2008. The offspring were slaughtered at 40 kg (male pigs) and 100 kg to 140 kg (female pigs andcastrates). The two breeds were compared regarding daily gain, feed conversion rate, animal behaviour,the thickness of the back fat and the meat, meat colour and sensory profile....... Preliminary results from thefirst year of study showed e.g. 37% lower daily gain from birth to slaughter, 30% higher back fat thicknessand 11% lower meat percentage in the Black-Spotted female pigs compared to the modern genotype. Incontrary, the Black-Spotted pig produced redder and darker meat compared......It is important that organic pork differs markedly from conventional pork regarding taste, appearance and production methods in order to overcome the heavy price competition. That is the hypothesis behind thecurrent project. A seasonal outdoor rearing system based on a traditional and local breed...

  12. MRSA CC398 in the pig production chain

    NARCIS (Netherlands)

    Broens, E.M.; Graat, E.A.M.; Wolf, van der P.J.; Giessen, van de A.W.; Duijkeren, van E.; Wagenaar, J.A.; Nes, van A.; Mevius, D.J.; Jong, de M.C.M.

    2011-01-01

    In 2005, a distinct clone of methicillin resistant Staphylococcus aureus (MRSA CC398) was found in pigs and people in contact with pigs. The structure of the pig production chain in high technology pig husbandry enables pathogens to spread during animal trading, with an increasing prevalence in herd

  13. Evaluation of the sustainability of contrasted pig farming systems: development of a market conformity tool for pork products based on technological quality traits.

    Science.gov (United States)

    Gonzàlez, J; Gispert, M; Gil, M; Hviid, M; Dourmad, J Y; de Greef, K H; Zimmer, C; Fàbrega, E

    2014-12-01

    A market conformity tool, based on technological meat quality parameters, was developed within the Q-PorkChains project, to be included in a global sustainability evaluation of pig farming systems. The specific objective of the market conformity tool was to define a scoring system based on the suitability of meat to elaborate the main pork products, according to their market shares based on industry requirements, in different pig farming systems. The tool was based on carcass and meat quality parameters that are commonly used for the assessment of technological quality, which provide representative and repeatable data and are easily measurable. They were the following: cold carcass weight; lean meat percentage; minimum subcutaneous back fat depth at m. gluteus medius level, 45 postmortem and ultimate pH (measured at 24-h postmortem) in m. longissimus lumborum and semimembranosus; meat colour; drip losses and intramuscular fat content in a m. longissimus sample. Five categories of pork products produced at large scale in Europe were considered in the study: fresh meat, cooked products, dry products, specialties and other meat products. For each of the studied farming systems, the technological meat quality requirements, as well as the market shares for each product category within farming system, were obtained from the literature and personal communications from experts. The tool resulted in an overall conformity score that enabled to discriminate among systems according to the degree of matching of the achieved carcass and meat quality with the requirements of the targeted market. In order to improve feasibility, the tool was simplified by selecting ultimate pH at m. longissimus or semimembranosus, minimum fat thickness measured at the left half carcass over m. gluteus medius and intramuscular fat content in a m. longissimus sample as iceberg indicators. The overall suitability scores calculated by using both the complete and the reduced tools presented good

  14. Seasonal organic pig production with a local breed

    DEFF Research Database (Denmark)

    Kongsted, Anne Grete; Claudi-Magnussen, C.; Horsted, Klaus

    2009-01-01

    It is important that organic pork differs markedly from conventional pork regarding taste, appearance and production methods in order to overcome the heavy price competition. That is the hypothesis behind thecurrent project. A seasonal outdoor rearing system based on a traditional and local breed...... is believed to bea feasible strategy for producing organic pork with high credibility and superior eating quality. The studyincluded a comparison between a modern genotype and the Danish Black-Spotted pig which does almostnot exist in the modern pig production of today. 17 gilts farrowed outdoors in April...... to the modern genotype and thefat of the Black-Spotted pig was characterised as having a special nutty taste. In conclusion, preliminaryresults indicate that the local breed differs markedly with respect to several meat quality aspects comparedto the modern breed but also shows clear disadvantages regarding...

  15. The environmental impacts of the production of concentrated feed: the case of pig feed in Bretagne

    NARCIS (Netherlands)

    Werf, H.M.G.; Petit, J.; Sanders, J.

    2005-01-01

    Pig production systems often depend to a large extent on concentrated feed imported from outside the farm. This study used the Life Cycle Assessment (LCA) method to assess major environmental impacts associated with the production and on-farm delivery of concentrated feed for pigs. Feed composition

  16. The environmental impacts of the production of concentrated feed: the case of pig feed in Bretagne

    NARCIS (Netherlands)

    Werf, H.M.G.; Petit, J.; Sanders, J.

    2005-01-01

    Pig production systems often depend to a large extent on concentrated feed imported from outside the farm. This study used the Life Cycle Assessment (LCA) method to assess major environmental impacts associated with the production and on-farm delivery of concentrated feed for pigs. Feed composition

  17. Age distribution of porcine sapovirus asymptomatic infection and molecular evidence of genogroups GIII and GIX? circulation in distinct Brazilian pig production systems.

    Science.gov (United States)

    Valente, Cecília Souza; Alfieri, Alice Fernandes; Barry, Aline Fernandes; Leme, Raquel Arruda; Lorenzetti, Elis; Alfieri, Amauri Alcindo

    2016-01-01

    This study aimed to evaluate the natural infection by SaV in pigs of different categories of production cycle in an important Brazilian pig-producing region. Faecal samples (n = 169) of suckling, post-weaning, finisher and breeder pig categories were analysed. Animals were from five farrow-to-weaning and nine grower-to-finish commercial pig farms. The RT-PCR assay was performed targeting the partial RNA-dependent RNA polymerase (RdRp) gene of porcine SaV genome. The virus was detected in 23.7% (40/169) of faecal samples and in 10/14 (5/5 farrow-to-weaning; 5/9 grower-to-finish) of pig farms evaluated. Porcine SaV was most frequently (p < 0.05) detected in pigs at post-weaning than in grower-to-finish and breeder categories. The phylogenetic analysis revealed that the porcine SaV strains belong to the GIII and GIX? genogroups. This study showed that the porcine SaV GIII genogroup has spread in the pig herds and provides the first evidence of GIX? genogroup circulation in South America.

  18. Research trends in outdoor pig production — A review

    Directory of Open Access Journals (Sweden)

    Hyun-Suk Park

    2017-09-01

    Full Text Available Since the industrialization of swine production in the late 1900s, swine farms in the United States, as well as in Europe, have largely become consolidated. Pig farms became larger in size but fewer in number, with 91% of market pigs being produced by large operations with 5,000 or more pigs on-site in the US, and only 3% of the total utilized agricultural land representing organic farming. Such change in the market made it difficult for small farmers to stay competitive, forcing them to find alternative ways to reduce the cost of production and increase profit using the outdoor production system. In contrast to the indoor confinement system, outdoor production system uses pasture-based units and/or deep-bedded hoop structures that promote animal welfare and environmental sustainability with a lower capital investment. In accord with the growing concern for animal and environmental welfare and food safety by the consumers, small farmers practicing an outdoor production system are seeing increased opportunities for marketing their products in the pork niche market. Unlike the general belief that the reproductive and growth performance measures of the outdoor sows and piglets are poorer in comparison with the animals reared indoors, studies showed that there was no significant difference in the performance measures, and some traits were even better in outdoor animals. Improved reproductive and production traits can increase the sustainability of outdoor farming. Present study reviewed the recent studies comparing the performance measures, meat quality and health of indoor and outdoor animals, as well as the efforts to improve the outdoor production system through changes in management such as hut types and breed of animals.

  19. Nutrition, key factor to reduce environmental load from pig production

    NARCIS (Netherlands)

    Aarnink, A.J.A.; Verstegen, M.W.A.

    2007-01-01

    In different parts of Europe animal production is highly concentrated. Pig production generally is the main animal production activity in these areas. Main concerns of these large numbers of pigs are the amount of surplus nutrients in excreta and gaseous losses to the environment. Main nutrients of

  20. Perception of the environmental impacts of current and alternative modes of pig production by stakeholder groups.

    Science.gov (United States)

    Petit, Jean; van der Werf, Hayo M G

    2003-08-01

    The current industrial pig production model is in crisis, due to its association with environmental pollution, doubtful product quality and lack of animal well-being. In Bretagne (France), a region of intensive pig production, a survey of seven stakeholder groups concerned with pig production was conducted, as part of a research programme dedicated to the assessment of the environmental impact of different modes of pig production. A very large majority of pig producers (93%) and their suppliers (100%) considers pig farms as an asset for the region, whereas a majority of scientists (58%), activists (78%) and consumers (54%) sees it as a handicap. Differences among stakeholder groups are minor with respect to the perceived importance of environmental and social issues. Stakeholders agree on the relative level of responsibility of pig farms with respect to specific problems. For all groups unpleasant odours and water quality come first with respect to responsibility, for most groups soil quality comes second, followed by product safety and air quality. For a future improved mode of pig production, 76% of pig producers and their suppliers prefer to adapt the current model, for all other groups the majority prefers an alternative model. While pig producers and their suppliers prefer a slurry-based housing system, all other groups prefer a straw-based system. Pig producers see the slurry-based system as technically superior and associate the straw-based system with poor working conditions, whereas consumers associate the slurry-based system in the first place with poor water quality and associate the straw-based system with less pollution. These results will be of use in the research programme on the environmental impact of modes of pig production, as they indicate the environmental impacts to be considered and their relative importance. The results will also help in deciding which options should be assessed. It is concluded that the poor image of the current pig

  1. Contribution of semen trait selection, artificial insemination technique, and semen dose to the profitability of pig production systems: A simulation study.

    Science.gov (United States)

    Gonzalez-Pena, Dianelys; Knox, Robert V; Rodriguez-Zas, Sandra L

    2016-01-15

    The economic impact of selection for semen traits on pig production systems and potential interaction with artificial insemination (AI) technique and semen dose remains partially understood. The objectives of this study were to compare the financial indicators (gross return, net profit, cost) in a three-tier pig production system under one of two selection strategies: a traditional strategy including nine paternal and maternal traits (S9) and an advanced strategy that adds four semen traits (S13). Maternal traits included the number of pigs born alive, litter birth weight, adjusted 21-day litter weight, and the number of pigs at 21 days, and paternal traits included days to 113.5 kg, back fat, average daily gain, feed efficiency, and carcass lean percentage. The four semen traits included volume, concentration, progressive motility of spermatozoa, and abnormal spermatozoa. Simultaneously, the impact of two AI techniques and a range of fresh refrigerated semen doses including cervical AI with 3 × 10(9) (CAI3) and 2 × 10(9) (CAI2) sperm cells/dose, and intrauterine AI with 1.5 × 10(9) (IUI1.5), 0.75 × 10(9) (IUI0.75), and 0.5 × 10(9) (IUI0.5) sperm cells/dose were evaluated. These factors were also evaluated using a range of farrowing rates (60%-90%), litter sizes (8-14 live-born pigs), and a selected semen collection frequency. The financial impact of the factors was assessed through simulation of a three-way crossbreeding system (maternal nucleus lines A and B and paternal nucleus line C) using ZPLAN. The highest return on investment (profit/cost) of boars was observed at 2.33 collections/wk (three periods of 24 hours between collections). Under this schedule, a significant (P < 0.0001) interaction between the selection strategy and the AI technique-dose combination was identified for the gross return; meanwhile, significant (P < 0.0001) additive effects of the selection strategy and AI technique-dose combination were observed for the net

  2. Helminth parasites in pigs: new challenges in pig production and current research highlights.

    Science.gov (United States)

    Roepstorff, A; Mejer, H; Nejsum, P; Thamsborg, S M

    2011-08-04

    Helminths in pigs have generally received little attention from veterinary parasitologists, despite Ascaris suum, Trichuris suis, and Oesophagostomum sp. being common worldwide. The present paper presents challenges and current research highlights connected with these parasites. In Danish swine herds, new indoor production systems may favour helminth transmission and growing knowledge on pasture survival and infectivity of A. suum and T. suis eggs indicates that they may constitute a serious threat to outdoor pig production. Furthermore, it is now evident that A. suum is zoonotic and the same may be true for T. suis. With these 'new' challenges and the economic impact of the infections, further research is warranted. Better understanding of host-parasite relationships and A. suum and T. suis egg ecology may also improve the understanding and control of human A. lumbricoides and T. trichiura infections. The population dynamics of the three parasites are well documented and may be used to study phenomena, such as predisposition and worm aggregation. Furthermore, better methods to recover larvae have provided tools for quantifying parasite transmission. Thus, an on-going study using helminth naïve tracer pigs has surprisingly demonstrated that soil infectivity with A. suum and T. suis increases during the first 2-3 years after pasture contamination. Though all three helminth species stimulate the Th2 arm of the immune system, Oesophagostomum seems weakly immunogenic, perhaps via specific modulation of the host immune system. A. suum and T. suis potently modulate the host immune response, up-regulating Th2 and down-regulating Th1. As a consequence, A. suum may compromise the efficacy of certain bacterial vaccines, whereas T. suis, which establish only short-term in humans, is a favourite candidate for down-regulating autoimmune Th1-related diseases in man. Some basic research findings have offered new possibilities for future sustainable control measures. For example

  3. ω-3 PUFA rich camelina oil by-products improve the systemic metabolism and spleen cell functions in fattening pigs.

    Science.gov (United States)

    Taranu, Ionelia; Gras, Mihail; Pistol, Gina Cecilia; Motiu, Monica; Marin, Daniela E; Lefter, Nicoleta; Ropota, Mariana; Habeanu, Mihaela

    2014-01-01

    Camelina oil-cakes results after the extraction of oil from Camelina sativa plant. In this study, camelina oil-cakes were fed to fattening pigs for 33 days and its effect on performance, plasma biochemical analytes, pro-/anti-inflammatory mediators and antioxidant detoxifying defence in spleen was investigated in comparison with sunflower meal. 24 crossbred TOPIG pigs were randomly assigned to one of two experimental dietary treatments containing either 12% sunflower meal (treatment 1-T1), or 12.0% camelina oil-cakes, rich in polyunsaturated fatty acids ω-3 (ω-3 PUFA) (treatment 2-T2). The results showed no effect of T2 diet (camelina cakes) on feed intake, average weight gain or feed efficiency. Consumption of camelina diet resulted in a significant decrease in plasma glucose concentration (18.47%) with a trend towards also a decrease of plasma cholesterol. In spleen, T2 diet modulated cellular immune response by decreasing the protein and gene expression of pro-inflammatory markers, interleukin 1-beta (IL-1β), tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6) and interleukin (IL-8) and cyclooxigenase 2 (COX-2) in comparison with T1 diet. By contrast, T2 diet increased (Poil-cakes appears to be a potentially alternative feed source for pig which preserves a high content of ω-3 PUFA indicating antioxidant properties by the stimulation of detoxifying enzymes expression and the suppression of spleen pro-inflammatory markers.

  4. Selection for production and reproduction traits in pigs

    NARCIS (Netherlands)

    Vries, de A.G.

    1989-01-01

    Introduction

    Reproduction traits are important for piglet production, whereas production traits are important for fattening. Pig breeding organizations improve both groups of traits by selection in nucleus populations. Optimization of selection in these nucleus populations

  5. Improved Pig Production and Performance in the Tropics | Enem ...

    African Journals Online (AJOL)

    The consumption of white rather than red meat is being advocated ... The constraints to pig production in Nigeria are mainly based on social and religious beliefs. ... housing, feeding, health, and product marketing were discussed to enable a ...

  6. Intensive pig production and manure management in Beijing, North China Plain

    OpenAIRE

    Mendoza Huaitalla, Roxana

    2014-01-01

    China, at the forefront of the livestock revolution, has experienced a more industrialized change, with an increment of the large livestock farms and of the decoupling between the livestock and arable land. Meat production in China is dominated by pork, which comprises approximately 50% of worldwide pig production. The description of the pig husbandry and manure management systems in the large animal operations of the NCP is not widely available. In order to describe the status quo o...

  7. Análise econômica do Sistema Intensivo de Suínos Criados ao Ar Livre Economic analysis of an Outdoor Pig Production System

    Directory of Open Access Journals (Sweden)

    Denyse Maria Galvão Leite

    2001-04-01

    Full Text Available Foi instalado um Sistema Intensivo de Suínos Criados ao Ar Livre (SISCAL, na Estação Experimental de Pato Branco do Instituto Agronômico do Paraná, com o objetivo de determinar os custos de implantação e produção deste sistema, na região Sudoeste do Paraná. A área do SISCAL foi dimensionada para um plantel de 14 matrizes e um reprodutor, a qual foi dividida em piquetes para separar os animais por estágios fisiológicos (reprodução, gestação e creche. O custo de implantação foi baseado nos custos de mão-de-obra e dos materiais utilizados para instalação do sistema e confecção de alguns equipamentos, como também os custos da construção da fábrica e depósito de ração e dos equipamentos para confecção das rações.O custo total de implantação do SISCAL, excluindo o valor da terra, foi de US$ 6862,80, ou seja, US$ 490,20 por matriz instalada, em julho de 1997. A construção e os equipamentos da fábrica de ração foram os itens mais caros do custo de implantação. O custo total de produção por kg de leitão desmamado produzido foi US$ 0,617, em junho de 1999. Neste período, foi possível obter 18,81% de lucro líquido por kg de leitão produzido.An Outdoor Pig Production System (SISCAL, at the Experimental Station Agronomic Institute of Paraná State was used with the objective to determine the establishment and production costs of this system in the Southwestern region of Paraná. The SISCAL area was dimensioned to a 14 sows and one boar herd, which was divided in paddocks to separate the animals per physiological stage (reproduction, gestation and nursery. The establishment cost was based on the labor, material used for the system installation and the manufacturing of some equipment, as well as the feed plant and storage construction costs and equipments for the ration production. The total establishment observed cost of the SISCAL, excluding capital value of land, was of US$ 6862.80 or US$ 490.20 per housed

  8. ω-3 PUFA rich camelina oil by-products improve the systemic metabolism and spleen cell functions in fattening pigs.

    Directory of Open Access Journals (Sweden)

    Ionelia Taranu

    Full Text Available Camelina oil-cakes results after the extraction of oil from Camelina sativa plant. In this study, camelina oil-cakes were fed to fattening pigs for 33 days and its effect on performance, plasma biochemical analytes, pro-/anti-inflammatory mediators and antioxidant detoxifying defence in spleen was investigated in comparison with sunflower meal. 24 crossbred TOPIG pigs were randomly assigned to one of two experimental dietary treatments containing either 12% sunflower meal (treatment 1-T1, or 12.0% camelina oil-cakes, rich in polyunsaturated fatty acids ω-3 (ω-3 PUFA (treatment 2-T2. The results showed no effect of T2 diet (camelina cakes on feed intake, average weight gain or feed efficiency. Consumption of camelina diet resulted in a significant decrease in plasma glucose concentration (18.47% with a trend towards also a decrease of plasma cholesterol. In spleen, T2 diet modulated cellular immune response by decreasing the protein and gene expression of pro-inflammatory markers, interleukin 1-beta (IL-1β, tumor necrosis factor alpha (TNF-α, interleukin 6 (IL-6 and interleukin (IL-8 and cyclooxigenase 2 (COX-2 in comparison with T1 diet. By contrast, T2 diet increased (P<0.05 in spleen the mRNA expression of antioxidant enzymes, catalase (CAT, superoxide dismutase (SOD, and glutathione peroxidase 1 (GPx1 by 3.43, 2.47 and 1.83 fold change respectively, inducible nitric oxide synthase (iNOS (4.60 fold, endothelial nitric oxide synthase (eNOS (3.23 fold and the total antioxidant level (9.02% in plasma. Camelina diet increased also peroxisome-proliferator activated receptor gamma (PPAR-γ mRNA and decreased that of mitogen-activated protein kinase 14 (p38α MAPK and nuclear factor of kappa light polypeptide gene enhancer in B-cells (NF-κB. At this level of inclusion (12% camelina oil-cakes appears to be a potentially alternative feed source for pig which preserves a high content of ω-3 PUFA indicating antioxidant properties by the stimulation

  9. Factors that influence pig production in Central Uganda - Case study of Nangabo Sub-County, Wakiso district

    Directory of Open Access Journals (Sweden)

    Dennis Muhanguzi

    2012-12-01

    Full Text Available Aim: To study the factors affecting pig production in Nangabo Sub-County, Wakiso District, Central Uganda. Materials and Methods: A total of 11% (1350/12783 of all households in the Nangabo Sub-County kept pigs. The systematic sampling technique was used to complete the list of all pig farming households. This was done by taking 10th farming household until 10% of all the farming households were sampled. A total of 135 farmers were interviewed with semi- structured questionnaire. Questionnaire data was entered into Microsoft Excel worksheet and trans-ferred into Statistical Package for Social Scientists Results: Results indicated that 49.6 % of the pigs were raised in the semi-intensive system while 31%, 12% and 8% of the farmers kept pigs on intensive, tethering and free range rearing systems respectively. Farmers that raised their pigs on what is hereby referred to as semi-intensive and intensive management systems allowed their pigs up to five and two hours of open foraging respectively. Ninety eight percent of the farmers provided housing to their pigs and the commonest pig houses were local mud houses and a few of them were tree shades. Majority of farmers (85.2% watered their pigs at least once a day. The major factors limiting pig production were; diseases and parasites mainly helminthosis and African swine fever (ASF. Others included; high costs of inputs, lack of capital, unstable availability of feed resources, inadequate advisory services and feed price fluctuation (maize bran, sow and weaner, lack of good quality breeding stock, poor and unorganized marketing, lack of enough land, high costs of veterinary medicines and inability to keep records. Additionally, lack of enough water and uncontrolled pig movement grossly limited the pig production in Wakiso district. Most Ugandans keep their pigs in peri-urban areas of Uganda. It was however, noted that there were important limitations for pig production in the country. To improve pig

  10. Increased Foraging in Outdoor Organic Pig Production-Modeling Environmental Consequences.

    Science.gov (United States)

    Jakobsen, Malene; Preda, Teodora; Kongsted, Anne Grete; Hermansen, John Erik

    2015-11-02

    Consumers' motivations for buying organic products include a wish of acquiring healthy, environmentally friendly products from production systems that also ensure a high level of animal welfare. However, the current Danish organic pig production faces important challenges regarding environmental impact of the system. High ammonia emissions arise from outdoor concrete areas with growing pigs and sows on pasture possess an increased risk of nitrogen (N) leaching. Direct foraging in the range area is suggested as a way to improve the nutrient efficiency at farm level and to support a more natural behavior of the pig. Thus, by modeling, we investigated the environmental consequences of two alternative scenarios with growing pigs foraging in the range area and different levels of crops available for foraging-grass-clover or a combination of Jerusalem artichokes and lucerne. It was possible to have growing pigs on free-range without increasing N leaching compared to the current practice. The alternative system with Jerusalem artichokes and lucerne (high integration of forage) showed the lowest carbon foot print with 3.12 CO₂ eq kg(-1) live weight pig compared to the current Danish pasture based system with 3.69 kg CO₂ eq kg(-1) live weight pig. Due to positive impact on soil carbon sequestration, the second alternative system based on grass-clover (low integration of forage) showed a similar carbon foot print compared to current practice with 3.68 kg CO₂ eq kg(-1) live weight pig. It is concluded that in practice there is room for development of organic farming systems where direct foraging plays a central role.

  11. Rethinking the history of modern agriculture: British pig production, c.1910-65.

    Science.gov (United States)

    Woods, Abigail

    2012-01-01

    This article uses a study of pig production in Britain, c.1910-65, to rethink the history of modern agriculture and its implications for human-animal relationships. Drawing on literature written by and for pig producers and experts, it challenges existing portrayals of a unidirectional, post-Second World War shift from traditional small-scale mixed farming to large, specialized, intensive systems. Rather, 'factory-style' pig production was already established in Britain by the 1930s, and its fortunes waxed and waned over time in relation to different kinds of outdoor production, which was still prominent in the mid-1960s. In revealing that the progressive proponents of both indoor and outdoor methods regarded them as modern and efficient, but defined and pursued these values in quite different ways, the article argues for a more historically situated understanding of agricultural modernity. Analysis reveals that regardless of their preferred production system, leading experts and producers were keen to develop what they considered to be natural methods that reflected the pig's natural needs and desires. They perceived pigs as active, sentient individuals, and believed that working in harmony with their natures was essential, even if this was, ultimately, for commercial ends. Such views contradict received accounts of modern farming as a utilitarian enterprise, concerned only with dominating and manipulating nature. They are used to argue that a romantic, moral view of the pig did not simply pre-date or emerge in opposition to modern agriculture, but, rather, was integral to it.

  12. Pig Production in Tanzania: a Critical Review

    Directory of Open Access Journals (Sweden)

    Wilson, RT.

    2014-01-01

    Full Text Available Tanzania's 1.58 million pigs represent 3.7 per cent of the national population of quadruped meat-producing animals. Some 99.5 per cent of pigs are kept by small producers in units averaging 3.04 animals (range 2-48. About 18 per cent of households with livestock own pigs, 93.7 per cent of these having a herd of less than 19 and 69.2 per cent own 9 or fewer head. Scavenging is the main feed source. Maize bran is the principle supplement but some owners provide oilseed cakes and minerals. Domestic pigs are not indigenous to Tanzania and derive mainly from late 19th/early 20th century introductions. There have been few imports of breeding stock since 1961. Poor management, in-breeding, inadequate nutrition and rudimentary veterinary attention lead to low output from late ages at first farrowing, long inter-birth intervals, small litters, slow growth and high mortality. Government policy is not applied in practice. Animals are slaughtered in primitive private facilities or household compounds with little concern for welfare or hygiene, often with no official inspection. Pigs can make a greater contribution to society but public and private sectors must provide additional support with particular attention to management, nutrition, health, welfare and food safety to achieve this.

  13. Proteomic approaches to study the pig intestinal system.

    Science.gov (United States)

    Soler, Laura; Niewold, Theo A; Moreno, Ángela; Garrido, Juan Jose

    2014-03-01

    One of the major challenges in pig production is managing digestive health to maximize feed conversion and growth rates, but also to minimize treatment costs and to warrant public health. There is a great interest in the development of useful tools for intestinal health monitoring and the investigation of possible prophylactic/ therapeutic intervention pathways. A great variety of in vivo and in vitro intestinal models of study have been developed in the recent years. The understanding of such a complex system as the intestinal system (IS), and the study of its physiology and pathology is not an easy task. Analysis of such a complex system requires the use of systems biology techniques, like proteomics. However, for a correct interpretation of results and to maximize analysis performance, a careful selection of the IS model of study and proteomic platform is required. The study of the IS system is especially important in the pig, a species whose farming requires a very careful management of husbandry procedures regarding feeding and nutrition. The incorrect management of the pig digestive system leads directly to economic losses related suboptimal growth and feed utilization and/or the appearance of intestinal infections, in particular diarrhea. Furthermore, this species is the most suitable experimental model for human IS studies. Proteomics has risen as one of the most promising approaches to study the pig IS. In this review, we describe the most useful models of IS research in porcine and the different proteomic platforms available. An overview of the recent findings in pig IS proteomics is also provided.

  14. Dynamic production monitoring in pig herds II

    DEFF Research Database (Denmark)

    Bono, Claudia; Cornou, Cécile; Lundbye-Christensen, Søren

    2013-01-01

    Good management in animal production systems is becoming of paramount importance. The aim of this paper was to develop a dynamic monitoring system for farrowing rate. A farrowing rate model was implemented using a dynamic generalized linear model (DGLM). Variance components were pre-estimated using...

  15. BPEX Pig Health Scheme: a useful monitoring system for respiratory disease control in pig farms?

    Directory of Open Access Journals (Sweden)

    Holt Hannah R

    2011-12-01

    Full Text Available Abstract Background Respiratory diseases account for significant economic losses to the UK pig industry. Lesions indicative of respiratory disease in pig lungs at slaughter e.g. pneumonia and pleuritis are frequently recorded to assess herd health or provide data for epidemiological studies. The BPEX Pig Health Scheme (BPHS is a monitoring system, which informs producers of gross lesions in their pigs' carcasses at slaughter, enabling farm-level decisions to be made. The aim of the study was to assess whether information provided by the BPHS regarding respiratory lesions was associated with respiratory pathogens in the farm, farm management practices and each other. Results BPHS reports were obtained from a subset of 70 pig farms involved in a cross-sectional study conducted in 2008-09 investigating the epidemiology of post-weaning multi-systemic wasting syndrome. The reports were combined with data regarding the presence/absence of several pathogens in the herd and potential farm-level risk factors for respiratory disease. Principal component analysis (PCA performed on BPHS reports generated three principal components, explaining 71% of the total variance. Enzootic pneumonia score, severe pleurisy and acute pleuropneumonia had the highest loadings for the principal component which explained the largest percentage of the total variance (35% (BPHS component 1, it was thought that this component identifies farms with acute disease. Using the factor loadings a score for each farm for BPHS component 1 was obtained. As farms' score for BPHS component 1 increased, average carcass weight at slaughter decreased. In addition, farms positive for H1N2 and porcine reproductive and respiratory disease virus (PRRSV were more likely to have higher levels of severe and mild pleurisy reported by the BPHS, respectively. Conclusions The study found statistical associations between levels of pleurisy recorded by BPHS at slaughter and the presence H1N2 and PRRSV in

  16. Consumer perceptions - pork and pig production: Insights from France, England, Sweden and Denmark

    DEFF Research Database (Denmark)

    Ngapo, T. M.; Dransfield, E.; Martin, J.-F.

    2004-01-01

    Consumer focus groups in France, England, Sweden and Denmark were used to obtain insights into the decision-making involved in the choice of fresh pork and attitudes towards today's pig production systems. Many positive perceptions of pork meat were evoked. Negative images of the production syste...... ethnocentrism, to assure that such information is targeted to enhance consumer confidence....

  17. Evaluation of the sustainability of contrasted pig farming systems: economy.

    Science.gov (United States)

    Ilari-Antoine, E; Bonneau, M; Klauke, T N; Gonzàlez, J; Dourmad, J Y; De Greef, K; Houwers, H W J; Fabrega, E; Zimmer, C; Hviid, M; Van der Oever, B; Edwards, S A

    2014-12-01

    The aim of this paper is to present an efficient tool for evaluating the economy part of the sustainability of pig farming systems. The selected tool IDEA was tested on a sample of farms from 15 contrasted systems in Europe. A statistical analysis was carried out to check the capacity of the indicators to illustrate the variability of the population and to analyze which of these indicators contributed the most towards it. The scores obtained for the farms were consistent with the reality of pig production; the variable distribution showed an important variability of the sample. The principal component analysis and cluster analysis separated the sample into five subgroups, in which the six main indicators significantly differed, which underlines the robustness of the tool. The IDEA method was proven to be easily comprehensible, requiring few initial variables and with an efficient benchmarking system; all six indicators contributed to fully describe a varied and contrasted population.

  18. Consumer perceptions - pork and pig production: Insights from France, England, Sweden and Denmark

    DEFF Research Database (Denmark)

    Ngapo, T. M.; Dransfield, E.; Martin, J.-F.

    2004-01-01

    Consumer focus groups in France, England, Sweden and Denmark were used to obtain insights into the decision-making involved in the choice of fresh pork and attitudes towards today's pig production systems. Many positive perceptions of pork meat were evoked. Negative images of the production syste...... ethnocentrism, to assure that such information is targeted to enhance consumer confidence.......Consumer focus groups in France, England, Sweden and Denmark were used to obtain insights into the decision-making involved in the choice of fresh pork and attitudes towards today's pig production systems. Many positive perceptions of pork meat were evoked. Negative images of the production systems...... in use today were expressed, but rationalised in terms of consumer demands, market competition and by comparisons to previous systems of production. Knowledge of production systems appeared of little consequence in terms of any meat market potential as several groups freely remarked...

  19. Selection for production and reproduction traits in pigs.

    NARCIS (Netherlands)

    Vries, de A.G.

    1989-01-01

    IntroductionReproduction traits are important for piglet production, whereas production traits are important for fattening. Pig breeding organizations improve both groups of traits by selection in nucleus populations. Optimization of selection in these nucleus populations is important, becaus

  20. Aspects related to animal welfare in pig production

    Directory of Open Access Journals (Sweden)

    Tiago Vieira de Andrade

    2015-09-01

    Full Text Available This review aimed to highlight the importance of pig welfare and meat quality, as well as some critical points, solutions to improve the management of these animals for swine production. The animal welfare settings have been widely debated within the international scientific community in recent decades. A conceptual row is the most widely accepted animal welfare within a multidimensional approach, encompassing emotions, biological functioning and natural behavior. However, understood the concepts, another challenge that arises is how to properly measure the animal welfare under field conditions. The subject animal welfare is growing rapidly and gaining greater importance in livestock production, not only pigs, but of all the exploited classes.

  1. EFFICIENCY THE KEY TO FUTURE PIG PRODUCTION E.H. Kemm

    African Journals Online (AJOL)

    The continued production of pigs and pig products will no doubt greatly ..... Braude believes that the annual output of lean meat per sow can be increased from a ..... Department of Agricultural Technical Services. Pretoria. RIDGEON. R.F.. 1911 ...

  2. Effects of rearing system and genotype on skeletal growth and carcass traits of pigs

    Directory of Open Access Journals (Sweden)

    A. Manchisi

    2011-03-01

    Full Text Available A growing interest has been shown in alternative pig production because of the low capital cost of outdoor systems and awareness of niche marketing opportunities (Gentry et al., 2001. However, available data on productive performances and pork quality for free-range or confinementreared pigs widely vary among studies: several factors could be confounding results such as climatic and environmental conditions, genotypes evaluated or others (Kleinbeck and McGlone, 1999; Gentry et al., 2001...

  3. Anaerobic digestion of pig manure and glycerol from biodiesel production

    Directory of Open Access Journals (Sweden)

    Pakamas Chetpattananondh, Sumate Chaiprapat, Chaisri Suksaroj

    2015-01-01

    Full Text Available Increasing biodiesel production causes a surplus of glycerol. This work aims to investigate the crude glycerol pretreatment method and then apply the glycerol as a co-substrate with pig manure for anaerobic digestion. The optimum crude glycerol pretreatment method was acidification with 6% of H2SO4 that highest glycerol recovery was obtained with lowest cost. Co-digestions of glycerol and pig manure enhanced biogas and methane productions compared with mono-digestions. Biogas and methane productions in semi-continuous digestions were highly effected by OLR. The optimum OLR was 3.06 kg SCOD/m3 that biogas production was maintained at 3 L/d with methane composition of 72% and SCOD removal higher than 80%.

  4. Changes in Pig Production in China and Their Effects on Nitrogen and Phosphorus Use and Losses

    NARCIS (Netherlands)

    Bai, Z.H.; Ma, L.; Qin, W.; Chen, Q.; Oenema, O.; Zhang, F.S.

    2014-01-01

    China's pig production has increased manifold in the past 50 years, and this has greatly affected the nitrogen and phosphorus use and losses in the pig production sector. However, the magnitude of these changes are not well-known. Here, we provide an in-depth account of the changes in pig

  5. Socio-cultural sustainability of pig production: Citizen perceptions in the Netherlands and Denmark

    DEFF Research Database (Denmark)

    Boogaard, B.K.; boekhorst, L.J.S.; Oosting, S.J.

    2011-01-01

    refers to social perceptions of animal farming, including social appreciations and concerns of animal production systems. Integration of social demands and values in the production sector is a prerequisite to justify animal production within a society. The objective of the present study was therefore...... positive and six negative aspects on the farms for which they had to write a motivation. The qualitative analysis resulted in seven socio-cultural themes (SCT) of pig production namely: 1) meat production, 2) farm activities, 3) farm income, 4) animals, 5) housing system, 6) environment and nature, and 7...

  6. Determination of pig sex in meat and meat products using multiplex real time-PCR.

    Science.gov (United States)

    Abdulmawjood, A; Krischek, C; Wicke, M; Klein, G

    2012-07-01

    For specific production lines, European retail companies demand exclusively female pork meat. To control the quality of their suppliers the identification and a quantitative detection of the animal sex origin of the meat is therefore of importance for meat processors. To enable a fast and reliable detection of male pig meat, a real time-PCR-system was designed in the present study. This was based on the genes AMEL-X and AMEL-Y. The real time-PCR assay allowed the detection of male pig meat at a concentration of 1% yielding a detection probability of 100% while the detection probability investigating meat samples containing 0.1% male pig meat was 44.4%. The analytic sensitivity of this system was assessed to be PCR reaction. The assessment of the accuracy of the real time-PCR assay to correctly identify sex individuals was investigated with 62 pigs including males (n=29) and females (n=33) belonging to different breeds/lines. With the newly designed test all analysed animals were correctly sexed. No amplification was obtained with cow, goat, sheep, turkey and chicken genomic DNA. The presented assay can be used for sex diagnosis, for the detection of male pig meat and for meat quality control.

  7. Influence of genotype and feeding strategy on pig performance, plasma concentrations of micro nutrients, immune responses and faecal microbiota composition of growing-finishing pigs in a forage-based system

    DEFF Research Database (Denmark)

    Kongsted, Anne Grete; Nørgaard, Jan Værum; Jensen, Søren Krogh;

    2015-01-01

    ' ability to take advantage of direct foraging to cover their nutritional needs and how this interacts with breed and affects robustness. Pig performance, plasma concentration of micro nutrients, immune response and faecal microbiota composition were studied in 72 growing pigs (34 to 105 kg live weight......In free-range pig production it is important to reduce the input of nutrients from supplementary feed to reduce nutrient leaching and improve the resource efficiency of the system. A promising development might be to encourage foraging behaviour of the pigs. However, very little is known about pigs...

  8. The production of multi-transgenic pigs: update and perspectives for xenotransplantation.

    Science.gov (United States)

    Niemann, Heiner; Petersen, Bjoern

    2016-06-01

    The domestic pig shares many genetic, anatomical and physiological similarities to humans and is thus considered to be a suitable organ donor for xenotransplantation. However, prior to clinical application of porcine xenografts, three major hurdles have to be overcome: (1) various immunological rejection responses, (2) physiological incompatibilities between the porcine organ and the human recipient and (3) the risk of transmitting zoonotic pathogens from pig to humans. With the introduction of genetically engineered pigs expressing high levels of human complement regulatory proteins or lacking expression of α-Gal epitopes, the HAR can be consistently overcome. However, none of the transgenic porcine organs available to date was fully protected against the binding of anti-non-Gal xenoreactive natural antibodies. The present view is that long-term survival of xenografts after transplantation into primates requires additional modifications of the porcine genome and a specifically tailored immunosuppression regimen compliant with current clinical standards. This requires the production and characterization of multi-transgenic pigs to control HAR, AVR and DXR. The recent emergence of new sophisticated molecular tools such as Zinc-Finger nucleases, Transcription-activator like endonucleases, and the CRISPR/Cas9 system has significantly increased efficiency and precision of the production of genetically modified pigs for xenotransplantation. Several candidate genes, incl. hTM, hHO-1, hA20, CTLA4Ig, have been explored in their ability to improve long-term survival of porcine xenografts after transplantation into non-human primates. This review provides an update on the current status in the production of multi-transgenic pigs for xenotransplantation which could bring porcine xenografts closer to clinical application.

  9. The effects of clinoptilolite on piglet and heavy pig production

    Directory of Open Access Journals (Sweden)

    Archimede Mordenti

    2010-01-01

    Full Text Available To evaluate the effects of clinoptilolite on piglet and heavy pig production two separated trials have been performed. In the first trial 40 pigs of the initial body weight of 55 kg were used. Animals were homogeneously allocated to two groups: a control group traditionally fed and a clinoptilolite group in which feed contained the additive at 2%. Pigs were slaugh- tered at about 160 kg body weight. Blood samples were taken to determine blood urea nitrogen (BUN. In the second trial a total of 116 piglets from 12 litters was used. Six litters were fed from the 7th day of life a diet containing clinoptilolite at 2%. According to the dietary treatment of the suckling period, 84 weaned piglets were homogeneously allocated to two groups fed up to 33 kg body weight a diet containing or not clinoptilolite at 2%. In both trials daily weight gain, feed intake and pigs’ health were regularly recorded. The dietary inclusion of clinoptilolite at 2% did not resulted in any modification either of growing performances or of uraemia. Piglets on clinoptilolite diet showed a significant (P<0.05 improvement of faecal dry matter content. At slaughtering the dietary inclusion of clinoptilolite resulted in a trend towards an improvement of lean cuts yield and in a significant increase (P<0.05 of the ratio between lean and fat cuts. From our data it is sug- gested that clinoptilolite does not impair pig growing performances, determines a higher dry matter content of piglet fae- ces and improves carcass quality of heavy pigs with particular regard to lean cuts yield and lean to fat cuts ratio.

  10. Characteristics of Organic Pig Production and risk analysis concerning Toxoplasma infection

    NARCIS (Netherlands)

    Kijlstra, A.; Cornelissen, J.B.W.J.; Meerburg, B.G.

    2007-01-01

    A short written questionnaire was used to study certain characteristics of the organic pig production chain in The Netherlands and the circumstances on the farm that might play a role in the transmission of Toxoplasma infection to the pigs. Of the 81 certified organic slaughter pig farmers present i

  11. PHARMACODYNAMIC EFFECTS OF APPLICATION OF POLISILAR PREPARATION IN PIG PRODUCTION

    Directory of Open Access Journals (Sweden)

    Semenenko M. P.

    2015-02-01

    Full Text Available An accelerated growth of farm animals in order to obtain maximum productivity is possible due to introduction stimulating additives and preparations based on them into the rations. One of such tools is Polisilar - complex preparation, which includes components that have a positive impact on various aspects of the animal metabolism in the conditions of physiological disorders. At the same time components of the preparation due to its high bioavailability exhibit a potentiating effect in the body that allows getting a more pronounced effect on its use. Studies were conducted on pigs 3-3.5 months age and showed that the Polisilar preparation has a positive effect on the clinical and physiological status of the animals and exhibits a pronounced growth stimulating effect, increasing the weight gain of pigs by 14.3%. In addition use of the preparation in rations of growing pigs has a normalizing effect on red blood and blood forming organs, increasing the number of red blood cells by 18.7%, hemoglobin - by 24.5%. Polisilar stimulates protein, carbohydrate and mineral exchanges, stabilizes the functional activity of the liver, reducing the toxic load on hepatocytes. Therefore, the preparation Polisilar has a pronounced biological and pharmacological activity and can be used in animal husbandry as an effective stimulant

  12. Potential for using indigenous pigs in subsistence-oriented and market-oriented small-scale farming systems of Southern Africa.

    Science.gov (United States)

    Madzimure, James; Chimonyo, Michael; Zander, Kerstin K; Dzama, Kennedy

    2013-01-01

    Indigenous pigs in South Africa are a source of food and economic autonomy for people in rural small-scale farming systems. The objective of the study was to assess the potential of indigenous pigs for improving communal farmer's livelihoods and to inform policy-makers about the conservation of indigenous pigs. Data were collected from 186 small-scale subsistence-oriented households and 102 small-scale market-oriented households using interviews and direct observations. Ninety-three percent of subsistence-oriented and 82 % of market-oriented households kept indigenous pigs such as Windsnyer, Kolbroek and non-descript crosses with exotic pigs mainly for selling, consumption and investment. Farmers in both production systems named diseases and parasites, followed by feed shortages, inbreeding and abortions as major constraints for pig production. Diseases and parasites were more likely to be a constraint to pig production in subsistence-oriented systems, for households where the head was not staying at home and for older farmers. Market-oriented farmers ranked productive traits such as fast growth rate, good meat quality and decent litter size as most important selection criteria for pig breeding stock, while subsistence-oriented farmers ranked good meat quality first, followed by decent growth rate and by low feed costs. We conclude that there is high potential for using indigenous pigs in subsistence-oriented production systems and for crossbreeding of indigenous pigs with imported breeds in market-oriented systems.

  13. Accounting for uncertainty in the quantification of the environmental impacts of Canadian pig farming systems.

    Science.gov (United States)

    Mackenzie, S G; Leinonen, I; Ferguson, N; Kyriazakis, I

    2015-06-01

    The objective of the study was to develop a life cycle assessment (LCA) for pig farming systems that would account for uncertainty and variability in input data and allow systematic environmental impact comparisons between production systems. The environmental impacts of commercial pig production for 2 regions in Canada (Eastern and Western) were compared using a cradle-to-farm gate LCA. These systems had important contrasting characteristics such as typical feed ingredients used, herd performance, and expected emission factors from manure management. The study used detailed production data supplied by the industry and incorporated uncertainty/variation in all major aspects of the system including life cycle inventory data for feed ingredients, animal performance, energy inputs, and emission factors. The impacts were defined using 5 metrics-global warming potential, acidification potential, eutrophication potential (EP), abiotic resource use, and nonrenewable energy use-and were expressed per kilogram carcass weight at farm gate. Eutrophication potential was further separated into marine EP (MEP) and freshwater EP (FEP). Uncertainties in the model inputs were separated into 2 types: uncertainty in the data used to describe the system (α uncertainties) and uncertainty in impact calculations or background data that affects all systems equally (β uncertainties). The impacts of pig production in the 2 regions were systematically compared based on the differences in the systems (α uncertainties). The method of ascribing uncertainty influenced the outcomes. In eastern systems, EP, MEP, and FEP were lower (P systems (P systems, despite their aforementioned differences. In conclusion, a probabilistic approach was used to develop an LCA that systematically dealt with uncertainty in the data when comparing multiple environmental impacts measures in pig farming systems for the first time. The method was used to identify differences between Canadian pig production systems

  14. A system response to an outbreak of enzootic pneumonia in grow/finish pigs

    Science.gov (United States)

    2004-01-01

    Abstract A Mycoplasma hyopneumoniae-negative commercial swine production system broke with enzootic pneumonia at their grow/finish site in southern Manitoba in October, 2003. System responses included feed medication, depopulation, delayed shipment of pigs to the infected site, vaccination of at risk sow herds, and disinfection when grow/finish site depopulation was completed. PMID:15532888

  15. Impact of nutritional strategies on water productivity indicators for pigs

    Directory of Open Access Journals (Sweden)

    Julio Cesar Pascale Palhares

    2013-12-01

    Full Text Available The productivity of water is a poorly considered indicator in animal agriculture. This is because water is a resource still believed by persons in the production network to be abundant and of good quality. The aim of this study was to evaluate the impact of nutritional strategies in water productivity indicators for growing and slaughtering pigs. Five strategies were evaluated: control diet (T1, with a reduction in the level of crude protein (T2, phytase (T3, organic minerals (T4 and the three nutritional strategies combined (T5. The water productivity indicator is defined as the quantity of product by water used. The following indicators were calculated: total weight (kg L-1, cold carcass (kg L-1 lean carcass (L kg-1, and nutrition (kcal L-1. T5 showed the best productivities for each liter of water used. The total weight productivity in this treatment was 3.0 kg L-1, while in T1 was 2.5 kg L-1. T3 had the lowest productivities. The nutritional water productivities were 2,512, 2,763, 2,657, 2,814, and 3,039 kcal L-1, respectively for T1, T2, T3, T4, and T5. Nutritional strategies reduce the use of drinking water and therefore improve water productivities. The best productivities were observed when combining the strategies.

  16. Sustainable production and sales of meat from free-range pigs

    DEFF Research Database (Denmark)

    Bredahl, Lone; Andersen, Lone Schreiber

    2002-01-01

    are developed on a European level for the production of pork from outdoors production systems, which combine animal welfare with high quality of consumption and high quality of health. In cooperation with colleagues in France, Great Britain and Sweden, MAPP is examining the market potential of this type of meat......These years, the consumption of pork is not only stagnating in Denmark but also on many other European markets. This coincides with a rise in consumer demand for increased welfare among farm animals. In a project about sustainable production and sales of meat from free-range pigs, guidelines...

  17. Evaluation of chronic immune system stimulation models in growing pigs.

    Science.gov (United States)

    Rakhshandeh, A; de Lange, C F M

    2012-02-01

    Two experiments (EXPs) were conducted to evaluate models of immune system stimulation (ISS) that can be used in nutrient metabolism studies in growing pigs. In EXP I, the pig's immune response to three non-pathogenic immunogens was evaluated, whereas in EXP II the pig's more general response to one of the immunogens was contrasted with observations on non-ISS pigs. In EXP I, nine growing barrows were fitted with a jugular catheter, and after recovery assigned to one of three treatments. Three immunogens were tested during a 10-day ISS period: (i) repeated injection of increasing amounts of Escherichia coli lipopolysaccharide (LPS); (ii) repeated subcutaneous injection of turpentine (TURP); and (iii) feeding grains naturally contaminated with mycotoxins (MYCO). In EXP II, 36 growing barrows were injected repeatedly with either saline (n = 12) or increasing amounts of LPS (n = 24) for 7 days (initial dose 60 μg/kg body weight). Treating pigs with TURP and LPS reduced feed intake (P < 0.02), whereas feed intake was not reduced in pigs on MYCO. Average daily gain (ADG; kg/day) of pigs on LPS (0.50) was higher than that of pigs on TURP (0.19), but lower than that of pigs on MYCO (0.61; P < 0.01). Body temperature was elevated in pigs on LPS and TURP, by 0.8°C and 0.7°C, respectively, relative to pre-ISS challenge values (39.3°C; P < 0.02), but remained unchanged in pigs on MYCO. Plasma concentrations of interleukin-1β were increased in pigs treated with LPS and TURP (56% and 55%, respectively, relative to 22.3 pg/ml for pre-ISS; P < 0.01), but not in MYCO-treated pigs. Plasma cortisol concentrations remained unchanged for pigs on MYCO and TURP, but were reduced in LPS-treated pigs (30% relative to 29.8 ng/ml for pre-ISS; P < 0.05). Red blood cell glutathione concentrations were lower in TURP-treated pigs (13% relative to 1.38 μM for pre-ISS; P < 0.05), but were unaffected in pigs on LPS and MYCO. In EXP I, TURP caused severe responses including skin ulceration and

  18. Intraocular Pressure, Tear Production, and Ocular Echobiometry in Guinea Pigs (Cavia porcellus).

    Science.gov (United States)

    Rajaei, Seyed Mehdi; Mood, Maneli Ansari; Sadjadi, Reza; Azizi, Farzaneh

    2016-01-01

    The purpose of this study was to evaluate intraocular pressure (IOP) by means of rebound tonometry, to assess tear production by using the endodontic absorbent paper point tear test (EAPTT) and phenol red thread test (PRTT), and to determine the effects of time of day on IOP and tear production in guinea pigs. The study population comprised 24 healthy adult guinea pigs (12 male, 12 female; 48 eyes) of different breeds and ranging in age from 12 to 15 mo. IOP and tear production were measured at 3 time points (0700, 1500, and 2300) during a 24-h period. Overall values (mean ± 1 SD) were: IOP, 6.81 ± 1.41 mm Hg (range, 4.83 to 8.50); PRTT, 14.33 ± 1.35 mm (range, 12.50 to 16.83); and EAPTT, 8.54 ± 1.08 mm (range, 7.17 to 10.0 mm). In addition, ultrasound biometry was performed by using a B-mode system with linear 8-MHz transducer. This study reports reference values for IOP and tear production in guinea pigs.

  19. Delayed development of systemic immunity in preterm pigs as a model for preterm infants

    DEFF Research Database (Denmark)

    Nguyen, Duc Ninh; Jiang, Pingping; Frøkiær, Hanne;

    2016-01-01

    Preterm neonates are highly sensitive to systemic infections in early life but little is known about systemic immune development following preterm birth. We hypothesized that preterm neonates have immature systemic immunity with distinct developmental trajectory for the first several weeks of life......, relative to those born at near-term or term. Using pigs as a model, we characterized blood leukocyte subsets, antimicrobial activities and TLR-mediated cytokine production during the first weeks after preterm birth. Relative to near-term and term pigs, newborn preterm pigs had low blood leukocyte counts......, poor neutrophil phagocytic rate, and limited cytokine responses to TLR1/2/5/7/9 and NOD1/2 agonists. The preterm systemic responses remained immature during the first postnatal week, but thereafter showed increased blood leukocyte numbers, NK cell proportion, neutrophil phagocytic rate and TLR2...

  20. Delayed development of systemic immunity in preterm pigs as a model for preterm infants

    DEFF Research Database (Denmark)

    Nguyen, Duc Ninh; Jiang, Pingping; Frøkiær, Hanne

    2016-01-01

    Preterm neonates are highly sensitive to systemic infections in early life but little is known about systemic immune development following preterm birth. We hypothesized that preterm neonates have immature systemic immunity with distinct developmental trajectory for the first several weeks of life......, relative to those born at near-term or term. Using pigs as a model, we characterized blood leukocyte subsets, antimicrobial activities and TLR-mediated cytokine production during the first weeks after preterm birth. Relative to near-term and term pigs, newborn preterm pigs had low blood leukocyte counts......, poor neutrophil phagocytic rate, and limited cytokine responses to TLR1/2/5/7/9 and NOD1/2 agonists. The preterm systemic responses remained immature during the first postnatal week, but thereafter showed increased blood leukocyte numbers, NK cell proportion, neutrophil phagocytic rate and TLR2...

  1. Consumer perceptions: pork and pig production. Insights from France, England, Sweden and Denmark.

    Science.gov (United States)

    Ngapo, T M; Dransfield, E; Martin, J-F; Magnusson, M; Bredahl, L; Nute, G R

    2004-01-01

    Consumer focus groups in France, England, Sweden and Denmark were used to obtain insights into the decision-making involved in the choice of fresh pork and attitudes towards today's pig production systems. Many positive perceptions of pork meat were evoked. Negative images of the production systems in use today were expressed, but rationalised in terms of consumer demands, market competition and by comparisons to previous systems of production. Knowledge of production systems appeared of little consequence in terms of any meat market potential as several groups freely remarked that there was no link between the negative images of production methods and their purchase behaviour. The groups were clearly confused and mistrusted the limited information available at the point of purchase. Careful consideration should be given to meat labelling, in particular taking account of the evident consumer ethnocentrism, to assure that such information is targeted to enhance consumer confidence.

  2. Deletion of pigR gene in Monascus ruber leads to loss of pigment production.

    Science.gov (United States)

    Xie, Nana; Liu, Qingpei; Chen, Fusheng

    2013-09-01

    Pigments produced by Monascus are traditional food colorants and are widely used as dietary supplements. Since genes involving in pigment biosynthesis have not been reported, we describe the identification of a putative pigment-regulatory gene (pigR) obtained by molecular analysis of an albino strain of Monascus ruber M7. In the pigR-deleted strain (ΔpigR), neither the pigments nor pigR expression were detected by HPLC or reverse-transcription PCR, respectively, whereas the introduction of the pigR, together with a constitutive trpC promoter into ΔpigR, caused it to produce 5.4 U of red pigments/g dry mycelia, about 12-fold higher than Monascus ruber M7 (0.46 U/g dry mycelia). Thus pigR up-regulates pigment production in Monascus ruber M7.

  3. Embedded Controller Design for Pig Stable Ventilation Systems

    DEFF Research Database (Denmark)

    Jessen, Jan Jacob

    This thesis focuses on zone based climate control in pig stables and how to implement climate controllers in a new range of products. The presented controllers are based on simple models of climate dynamics and simple models of actuators. The implementation uses graphical point and click features...

  4. Relationship between biosecurity and production/antimicrobial treatment characteristics in pig herds.

    Science.gov (United States)

    Laanen, M; Persoons, D; Ribbens, S; de Jong, E; Callens, B; Strubbe, M; Maes, D; Dewulf, J

    2013-11-01

    The biosecurity status of 95 breeder-finisher pig herds was quantified using a risk-based weighted scoring system. Data relating to herd-, farmer- and production-characteristics and to the prophylactic use of antimicrobials were also collected. The average external biosecurity score (measures to prevent pathogens from entering a herd) was 65 (range, 45-89) and the average internal score (measures to reduce the within-herd spread of pathogens) was 52 (range, 18-87). External scores were positively associated with herd size, while internal scores were negatively associated with both 'age of buildings' and 'years of experience of the farmer', indicating that biosecurity is generally better implemented in larger herds, in more modern facilities and by younger farmers. External and internal biosecurity scores were positively associated with daily weight gain and negatively associated with feed conversion ratio of fattening pigs. Internal scores were negatively associated with disease treatment incidence, suggesting that improved biosecurity might help in reducing the amount of antimicrobials used prophylactically. This study demonstrates and quantifies a clear link between biosecurity and both production- and antimicrobial treatment-related criteria in pig herds.

  5. Environmental and economic impacts of using co-products in the diets of finishing pigs in Brazil

    NARCIS (Netherlands)

    Ali, Beshir M.; Zanten, van Hannah H.E.; Berentsen, Paul; Bastiaansen, John W.M.; Bikker, Paul; Lansink, Alfons Oude

    2017-01-01

    Alternative feed sources are required to improve the environmental and economic sustainability of current pig production systems and to reduce the competition for cropland between the feed and food sectors. The objective of this study was to assess the environmental and economic impacts of utilizing

  6. Heterogeneous computing for a real-time pig monitoring system

    Science.gov (United States)

    Choi, Younchang; Kim, Jinseong; Kim, Jaehak; Chung, Yeonwoo; Chung, Yongwha; Park, Daihee; Kim, Hakjae

    2017-06-01

    Video sensor data has been widely used in automatic surveillance applications. In this study, we present a method that automatically detects pigs in a pig room by using depth information obtained from a Kinect sensor. For a real-time implementation, we propose a means of reducing the execution time by applying parallel processing techniques. In general, most parallel processing techniques have been used to parallelize a specific task. In this study, we consider parallelization of an entire system that consists of several tasks. By applying a scheduling strategy to identify a computing device for each task and implementing it with OpenCL, we can reduce the total execution time efficiently. Experimental results reveal that the proposed method can automatically detect pigs using a CPU-GPU hybrid system in real time, regardless of the relative performance between the CPU and GPU.

  7. Managing sulphur content of pig diet to control further sulphides production during pig slurry anaerobic storage

    OpenAIRE

    Peu, P.; Picard, S.; Dourmad, J.Y.; Dabert, P.

    2010-01-01

    International audience; The mixing of different raw materials during formulation strategies of pig feeding influences sulphurs food intake and further sulphur excretion by the animals. Increased amount of sulphur in manure can result in toxicity and odour problems during storage as well as potential inhibition problems in case of anaerobic digestion treatment. The objective of this study was to determine the behaviour of different sulphur feed contents on pig excretion via faeces and urine, t...

  8. Antimicrobial Consumption in Medicated Feeds in Vietnamese Pig and Poultry Production

    OpenAIRE

    Van Cuong, Nguyen; Nhung, Nguyen Thi; Nghia, Nguyen Huu; Mai Hoa, Nguyen Thi; Trung, Nguyen Vinh; Thwaites, Guy; Carrique-Mas, Juan

    2016-01-01

    Antimicrobials are extensively used as growth promoters in animal feeds worldwide, but reliable estimates are lacking. We conducted an internet-based survey of commercial chicken and pig feed products officially approved for sale in Vietnam over the period March–June 2015. Information on the antimicrobial contents in feed products, alongside animal production data, was used to estimate in-feed antimicrobial consumption to produce one kilogram of live animal (chicken, pig), as well as to estim...

  9. Antimicrobial Consumption in Medicated Feeds in Vietnamese Pig and Poultry Production

    OpenAIRE

    Cuong, Nguyen Van; Nhung, Nguyen Thi; Nghia, Nguyen Huu; Mai Hoa, Nguyen Thi; Trung, Nguyen Vinh; Thwaites, Guy; Carrique-Mas, Juan

    2016-01-01

    Antimicrobials are extensively used as growth promoters in animal feeds worldwide, but reliable estimates are lacking. We conducted an internet-based survey of commercial chicken and pig feed products officially approved for sale in Vietnam over the period March–June 2015. Information on the antimicrobial contents in feed products, alongside animal production data, was used to estimate in-feed antimicrobial consumption to produce one kilogram of live animal (chicken, pig), as well as to estim...

  10. Odor from pig production: its relation to diet

    NARCIS (Netherlands)

    Dinh Phung, Le P.D.

    2006-01-01

    Keywords.Odor, Pigs, Diet, Manure, Protein, Amino Acids, Fermentable carbohydratesOdor from pig manure creates a serious nuisance for people li

  11. Microbial production of volatile sulphur compounds in the large intestine of pigs fed two different diets

    DEFF Research Database (Denmark)

    Poulsen, Henrik Vestergaard; Jensen, Bent Borg; Finster, Kai

    2012-01-01

    Aims: To investigate the production of volatile sulphur compounds (VSC) in segments of the large intestine of pigs and to assess the impact of diet on this production. Methods and Results: Pigs were fed two diets based on either wheat and barley (STD) or wheat and dried distillers grains with sol......Aims: To investigate the production of volatile sulphur compounds (VSC) in segments of the large intestine of pigs and to assess the impact of diet on this production. Methods and Results: Pigs were fed two diets based on either wheat and barley (STD) or wheat and dried distillers grains...... significantly higher in the STD group. Conversely, the net methanethiol production rate was significantly higher in the DDGS-group, while no difference was observed for dimethyl sulphide. The number of sulphate reducing bacteria and total bacteria were determined by quantitative PCR and showed a significant...

  12. Influence of housing systems on stillbirth and mortality rate in preweaning pigs farrowed by different gilt breeds

    Directory of Open Access Journals (Sweden)

    Wiebke Wellbrock

    2010-01-01

    Full Text Available The aim of this paper was to investigate the influence of housing systems on stillbirth and mortality rate in preweaning pigs farrowed by different gilt breeds. The investigation included first farrowings of 225 Large White (LW gilts, 297 Swedish Landrace (SL gilts and 260 crossbreed gilts (LWxSL in the period of five years. Despite of positive influence of straw through decreased number of stillborn pigs, especially in LW gilts, in further process of production until weaning, less appropriate microclimatic conditions developed in farrowing houses with straw, especially if accompanied with poor ventilation, can result with higher number of perished pigs and lower number of weaned pigs. Most sensible to unsuitable microclimatic conditions in straw bedded farrowing houses, according to the number of perished pigs before weaning, were the pigs from SL gilts, while the pigs from crossbreed gilts LWxSL were the most resistant. These results suggest that adding straw requires special attention on influence of such production on microclimate conditions in pigs’ biozone in order to maintain its initial benefits in the time of farrowing through the whole preweaning period, especially in pure breeds of gilts.

  13. Evaluation of the sustainability of contrasted pig farming systems: breeding programmes.

    Science.gov (United States)

    Rydhmer, L; Gourdine, J L; de Greef, K; Bonneau, M

    2014-12-01

    The sustainability of breeding activities in 15 pig farming systems in five European countries was evaluated. One conventional and two differentiated systems per country were studied. The Conventional systems were the standard systems in their countries. The differentiated systems were of three categories: Adapted Conventional with focus on animal welfare, meat quality or environment (five systems); Traditional with local breeds in small-scale production (three systems) and Organic (two systems). Data were collected with a questionnaire from nine breeding organisations providing animals and semen to the studied farming systems and from, on average, five farmers per farming system. The sustainability assessment of breeding activities was performed in four dimensions. The first dimension described whether the market for the product was well defined, and whether the breeding goal reflected the farming system and the farmers' demands. The second dimension described recording and selection procedures, together with genetic change in traits that were important in the system. The third dimension described genetic variation, both within and between pig breeds. The fourth dimension described the management of the breeding organisation, including communication, transparency, and technical and human resources. The results show substantial differences in the sustainability of breeding activities, both between farming systems within the same category and between different categories of farming systems. The breeding activities are assessed to be more sustainable for conventional systems than for differentiated systems in three of the four dimensions. In most differentiated farming systems, breeding goals are not related to the system, as these systems use the same genetic material as conventional systems. The breeds used in Traditional farming systems are important for genetic biodiversity, but the small scale of these systems renders them vulnerable. It is hoped that, by

  14. Evaluating environmental impacts of contrasting pig farming systems with life cycle assessment.

    Science.gov (United States)

    Dourmad, J Y; Ryschawy, J; Trousson, T; Bonneau, M; Gonzàlez, J; Houwers, H W J; Hviid, M; Zimmer, C; Nguyen, T L T; Morgensen, L

    2014-12-01

    Environmental impacts of 15 European pig farming systems were evaluated in the European Union Q-PorkChains project using life cycle assessment. One conventional and two non-conventional systems were evaluated from each of the five countries: Denmark, The Netherlands, Spain, France and Germany. The data needed for calculations were obtained from surveys of 5 to 10 farms from each system. The systems studied were categorised into conventional (C), adapted conventional (AC), traditional (T) and organic (O). Compared with C systems, AC systems differed little, with only minor changes to improve meat quality, animal welfare or environmental impacts, depending on the system. The difference was much larger for T systems, using very fat, slow-growing traditional breeds and generally outdoor raising of fattening pigs. Environmental impacts were calculated at the farm gate and expressed per kg of pig live weight and per ha of land used. For C systems, impacts per kg LW for climate change, acidification, eutrophication, energy use and land occupation were 2.3 kg CO2-eq, 44.0 g SO2-eq, 18.5 g PO4-eq, 16.2 MJ and 4.1 m2, respectively. Compared with C, differences in corresponding mean values were +13%, +5%, 0%, +2% and +16% higher for AC; +54%, +79%, +23%, +50% and +156% for T, and +4%, -16%, +29%, +11% and +121% for O. Conversely, when expressed per ha of land use, mean impacts were 10% to 60% lower for T and O systems, depending on the impact category. This was mainly because of higher land occupation per kg of pig produced, owing to feed production and the outdoor raising of sows and/or fattening pigs. The use of straw bedding tended to increase climate change impact per kg LW. The use of traditional local breeds, with reduced productivity and feed efficiency, resulted in higher impacts per kg LW for all impact categories. T systems with extensive outdoor raising of pigs resulted in markedly lower impact per ha of land used. Eutrophication potential per ha was substantially

  15. Is a 10-sow unit economically sustainable? A profitability assessment of productivity amongst small-holder pig farmers, Mpumalanga, South Africa.

    Science.gov (United States)

    Munzhelele, Priscilla; Oguttu, James W; Fasina, Folorunso O

    2016-05-12

    The majority of small-holder pig farmers in Mpumalanga had between 1- and 10-sow herds. The main aim of this study is to evaluate the current government agricultural intervention (supply of 10 sows and a boar) in terms of technical and economic feasibilities and ascertain whether the small-scale pig value chain system alleviates poverty. Data were obtained from 220 randomly selected small-holder pig farmers using a semi-structured questionnaire. The results showed that 58% farrowed ≤ 10 piglets/born/sow/litter, 44.2% practiced no weaning method and many fed swill and leftovers alone (41.6%). Pair-wise association revealed that the feeding of commercial feeds had a relationship with pigs in relatively good to very good body condition. Pigs in poor body condition were positively correlated with the feeding of swill alone. The economic models for the 10-sow unit proved that pig farming is unprofitable if the current management and feeding systems that operate in the commercial industry are utilised. However, only through a combination of cooperative systems, benefits of economies of scale, reduction of preweaning mortalities and structured government inputs can pig production be profitable at this scale of production.

  16. Voluntary ban on cephalosporin use in Danish pig production has effectively reduced extended-spectrum cephalosporinase-producing Escherichia coli in slaughter pigs

    DEFF Research Database (Denmark)

    Agersø, Yvonne; Aarestrup, Frank Møller

    2013-01-01

    Objectives To measure the effect of a voluntary ban on cephalosporin usage in the Danish pig production on the prevalence of extended-spectrum cephalosporinase (ESC)-producing Escherichia coli in pigs and pork.Methods Data on cephalosporin consumption were obtained from the VetStat database. For ...

  17. Supplementation with a Lactobacillus acidophilus fermentation product alters the metabolic response following a lipopolysaccharide challenge in weaned pigs

    Science.gov (United States)

    This study was designed to determine if feeding a Lactobacillus acidophilus fermentation product to weaned pigs would alter the metabolic response following a lipopolysaccharide (LPS) challenge. Pigs (n=30; 6.4+/-0.1 kg BW) were housed individually with ad libitum access to feed and water. Pigs were...

  18. Effect of Floor Space Allowance on Pig Productivity across Stages of Growth: A Field-scale Analysis.

    Science.gov (United States)

    Lee, Joon H; Choi, Hong L; Heo, Yong J; Chung, Yoon P

    2016-05-01

    A total of 152 pig farms were randomly selected from the five provinces in South Korea. During the experiment, the average temperature and relative humidity was 24.7°C and 74% in summer and 2.4°C and 53% in winter, respectively. The correlation between floor space allowance (FSA) and productivity index was analyzed, including non-productive sow days (NPD), number of weaners (NOW), survival rate (SR), appearance rate of A-grade pork (ARA), and days at a slaughter weight of 110 kg (d-SW) at different growth stages. The objectives of the present study were i) to determine the effect of FSA on the pig productivity index and ii) to suggest the minimum FSA for pigs based on scientific baseline data. For the pregnant sow, NPD could be decreased if pregnant sows were raised with a medium level (M) of FSA (3.10 to 3.67 m(2)/head) while also keeping the pig house clean which improves hygiene, and operating the ventilation system properly. For the farrowing sows, the NOW tended to decrease as the FSA increased. Similarly, a high level of FSA (H) is significantly negative with weaner SR of farrowing sows (p-value = 0.017), indicating this FSA tends to depress SR. Therefore, a FSA of 2.30 to 6.40 m(2)/head (very low) could be appropriate for weaners because a limited space can provide a sense of security and protection from external interruptions. The opposite trend was observed that an increase in floor space (>1.12 m(2)/head) leads to increase the SR of growing pigs. For the fattening pigs, H level of FSA was negatively correlated with SR, but M level of FSA was positively correlated with SR, indicating that SR tended to increase with the FSA of 1.10 to 1.27 m(2)/head. In contrast, ARA of male fattening pigs showed opposite results. H level of FSA (1.27 to 1.47 m(2)/head) was suggested to increase productivity because ARA was most affected by H level of space allowance with positive correlation (R(2) = 0.523). The relationship between the FSA and d-SW of fattening pigs was

  19. MICROCLIMATIC INFLUENCE AND PRODUCTIVITY OF PIGS WITH RESPECT TO DIFFERENT CONDITIONS OF FATTENING

    Directory of Open Access Journals (Sweden)

    V. Margeta

    2005-12-01

    Full Text Available The aim of this research was to compare productivity traits of two pig genotypes fattened in two different ways, on deep litter and in a conventional way (flat deck, and to determine whether there was microclimatic influence on the productivity traits of fattening pigs. The research was carried out on 57 fattening pigs of both sex, divided into two groups by their housing. Each group consisted of pigs of two genotypes, i.e. three-way crossbreeds of Large White and German Landrace (LW x GL in the dam line, and of German Landrace and Pietrain (P in the sire line. Pigs were fed ad libitum with isocaloric and isoprotein diets. During the whole experiment, temperature, air moisture, air circulation speed and the content of NH3 and CO2 were measured in pens. No statistically significant differences were recorded with respect to temperature, air moisture, air circulation speed and content of carbon dioxide (CO2 and ammonia (NH3 in the air in pens with deep litter and without it (P>0.05. Pigs housed without deep litter had statistically highly significant (P<0.01 higher final weights than pigs kept on deep litter. Pigs crossed with Pietrain as a terminal breed, kept in pens without deep litter, had statistically significantly higher (P<0.05 average daily gains than pigs of the same genotype kept on deep litter. In the finishing phase of fattening, group of pigs being kept in pens without deep litter had statistically higher average daily gains than pigs kept on deep litter (P<0.05. The way of fattening had statistically highly significant (P<0.001 influence on live weight of pigs in the first, second and fourth fattening phase. Average daily gains in the starting two fattening phases were significantly influenced by the way of fattening. Its influence was very highly significant (P<0.01 in the finishing phase of fattening. Noticeable effect of the genotype was determined only for live weights in the second phase of fattening. Genotype influence was not

  20. Production of Modularised Product Systems

    DEFF Research Database (Denmark)

    Jacobsen, Peter

    2004-01-01

    Abstract: To day, more and more products are customized. Trends are not only to sell a product to the customer, but to sell a product system. The system can either be a combination of physical products or physical products together with some kind of service. Customers get in this way not a product...... but a solution. Modularisation is one tool used in designing the products. Designing and controlling a production system making customized products in an economical way is not an easy task. In order to fulfil the Lean and Agile manufacturing philosophies the production is often carried out in networks. Here...... the decoupling point has a central role. The scope for this article is therefore to analyse the possibilities for using modularisation in designing and controlling a production system. How will the development of modularised product systems influence the production system? In the paper, a case will be used...

  1. Production of Modularised Product Systems

    DEFF Research Database (Denmark)

    Jacobsen, Peter

    2004-01-01

    Abstract: To day, more and more products are customized. Trends are not only to sell a product to the customer, but to sell a product system. The system can either be a combination of physical products or physical products together with some kind of service. Customers get in this way not a product...... but a solution. Modularisation is one tool used in designing the products. Designing and controlling a production system making customized products in an economical way is not an easy task. In order to fulfil the Lean and Agile manufacturing philosophies the production is often carried out in networks. Here...... the decoupling point has a central role. The scope for this article is therefore to analyse the possibilities for using modularisation in designing and controlling a production system. How will the development of modularised product systems influence the production system? In the paper, a case will be used...

  2. Spatiotemporal trends in Canadian domestic wild boar production and habitat predict wild pig distribution

    DEFF Research Database (Denmark)

    Michel, Nicole; Laforge, Michel; van Beest, Floris

    2017-01-01

    Understanding source dynamics of invasive species is crucial to their management. Free-ranging wild pigs (Sus scrofa) have caused considerable ecological and agricultural damage throughout their global range, including Canada. Objectives were to assess the spatial and temporal patterns in domestic...... wild boar and test the propagule pressure hypothesis to improve predictive ability of an existing habitat-based model of wild pigs. We reviewed spatiotemporal patterns in domestic wild boar production across ten Canadian provinces during 1991–2011 and evaluated the ability of wild boar farm...... distribution to improve predictive models of wild pig occurrence using a resource selection probability function for wild pigs in Saskatchewan. Domestic wild boar production in Canada increased from 1991 to 2001 followed by sharp declines in all provinces. The distribution of domestic wild boar farms in 2006...

  3. Safety analysis report for packaging (onsite) sample pig transport system

    Energy Technology Data Exchange (ETDEWEB)

    MCCOY, J.C.

    1999-03-16

    This Safety Analysis Report for Packaging (SARP) provides a technical evaluation of the Sample Pig Transport System as compared to the requirements of the U.S. Department of Energy, Richland Operations Office (RL) Order 5480.1, Change 1, Chapter III. The evaluation concludes that the package is acceptable for the onsite transport of Type B, fissile excepted radioactive materials when used in accordance with this document.

  4. Production of transgenic pigs mediated by pseudotyped lentivirus and sperm.

    Directory of Open Access Journals (Sweden)

    Yongliang Zhang

    Full Text Available Sperm-mediated gene transfer can be a very efficient method to produce transgenic pigs, however, the results from different laboratories had not been widely repeated. Genomic integration of transgene by injection of pseudotyped lentivirus to the perivitelline space has been proved to be a reliable route to generate transgenic animals. To test whether transgene in the lentivirus can be delivered by sperm, we studied incubation of pseudotyped lentiviruses and sperm before insemination. After incubation with pig spermatozoa, 62±3 lentiviral particles were detected per 100 sperm cells using quantitative real-time RT-PCR. The association of lentivirus with sperm was further confirmed by electron microscopy. The sperm incubated with lentiviral particles were artificially inseminated into pigs. Of the 59 piglets born from inseminated 5 sows, 6 piglets (10.17% carried the transgene based on the PCR identification. Foreign gene and EGFP was successfully detected in ear tissue biopsies from two PCR-positive pigs, revealed via in situ hybridization and immunohistochemistry. Offspring of one PCR-positive boar with normal sows showed PCR-positive. Two PCR-positive founders and offsprings of PCR-positive boar were further identified by Southern-blot analysis, out of which the two founders and two offsprings were positive in Southern blotting, strongly indicating integration of foreign gene into genome. The results indicate that incubation of sperm with pseudotyped lentiviruses can incorporated with sperm-mediated gene transfer to produce transgenic pigs with improved efficiency.

  5. Evaluation of immune system function in neonatal pigs born vaginally or by Cesarean section.

    Science.gov (United States)

    Daniel, J A; Carroll, J A; Keisler, D H; Kojima, C J

    2008-07-01

    Full term crossbred sows were selected to study the interaction of the immune system, hypothalamus-pituitary-adrenal axis, and growth in pigs born by Cesarean section (c-section; n=4 sows) or vaginal birth (n=4 sows). Gestation length and birth weight did not differ between vaginal birth and c-section pigs (P=0.34 and 0.62, respectively). Blood and tissue samples were collected from 44 pigs at birth. Forty-five pigs were weaned at 13 d. On d 14, pigs received an i.p. injection of lipopolysaccaride (LPS; 150 microg/kg) or saline at min 0, and blood samples were collected at -20, -10, 0, 5, 10, 20, 40, 60, 90, and 120 min. Vaginal birth pigs had 21% greater average daily gain than c-section pigs on d 14 (Ppigs at birth (P0.22). Basal serum concentrations of TNF-alpha tended to be greater in c-section vs vaginal birth pigs at 14 d (P=0.0967); however, basal serum concentrations of IFN-gamma tended to be lower in c-section pigs vs vaginal birth pigs at 14 d (P=0.0787). Expression of interleukin (IL)-6, IL-6 receptor, IL-1beta, and TNF-alpha mRNA did not differ between vaginal birth and c-section pigs but changed in an age and tissue dependent manner. Thus, reduced growth rate of c-section pigs is associated with altered immune system function.

  6. Influence of production factors on feed intake and feed conversion ratio of grow-finishing pigs

    Directory of Open Access Journals (Sweden)

    Caio Abércio da Silva

    2017-05-01

    Full Text Available The aim of this study was to identify and quantify, through mathematical models, the production factors of grow-finishing (GF phases that influence the daily feed intake (DFI and feed conversion ratio (FCR in pigs. Sixty-five GF farms were evaluated between 2010 and 2013, linked to a cooperative system located in the western Parana State, Brazil, representing 463 batches, with a mean of 642.79 ± 363.29 animals per batch, equalling approximately 300,000 animals. Forty production factors were considered that related to management, sanitation, installations and equipment, nutrition, genetics and environment on the farms. The DFI was influenced by the barn's position relative to the sun (P = 0.048, initial body weight (P < 0.0001 and final body weight (P < 0.0001. It was observed that the FCR was influenced by the barn’s position relative to the sun (P = 0.0001, the use of humidifiers/misting (P = 0.03, the presence of composters (P = 0.006, trees on the sides of barns (P < 0.045, the initial body weight of the pigs (P < 0.0001 and duration of the grow-finishing phase (P < 0.0001. The variables selected in the models explained approximately 44 and 20% of the total variance in the DFI and FCR, respectively, demonstrating that this resource is a good tool for interpreting the factors related to the parameters evaluated.

  7. Relationships between longevity, lifetime productivity, carcass traits and conformation in Polish maternal pig breeds.

    Science.gov (United States)

    Sobczyńska, M; Blicharski, T; Tyra, M

    2013-10-01

    The objective of this study was to obtain heritability estimates for longevity (length of life, length of productive life, number of litters) and lifetime productivity traits (lifetime pig production, lifetime pig efficiency, lifetime litter efficiency) and genetic correlation between them and litter size at first farrowing, growth (ADG), backfat thickness (BF), loin depth, lean meat percentage (LMP), phenotypic selection index (PSI), and exterior in 19423 Polish Landrace (L) and 16049 Polish Large White (LW) sows. Heritabilities for longevity and lifetime productivity traits were 0.10-0.13 for L sows and 0.09-0.11 for LW sows depending on the trait definition. The genetic correlations among these traits were all high and positive, ranging from 0.76 to 0.99. Antagonistic genetic correlations (-0.21 to -0.26) were found between longevity traits and PSI and LMP in LW sows, while in L sows the respective parameters were lower and not significant for length of productive life. The number of live-born piglets in the first litter was positively correlated with lifetime pig production and lifetime pig efficiency in both breeds. The genetic correlations of longevity and lifetime pig production with ADG, BF, loin depth and exterior were small, and in most cases, not significant.

  8. Impact of swine reproductive technologies on pig and global food production.

    Science.gov (United States)

    Knox, Robert V

    2014-01-01

    Reproductive technologies have dramatically changed the way pigs are raised for pork production in developed and developing countries. This has involved such areas as pigs produced/sow, more consistent pig flow to market, pig growth rate and feed efficiency, carcass yield and quality, labor efficiency, and pig health. Some reproductive technologies are in widespread use for commercial pork operations [Riesenbeck, Reprod Domest Anim 46:1-3, 2011] while others are in limited use in specific segments of the industry [Knox, Reprod Domest Anim 46:4-6, 2011]. Significant changes in the efficiency of pork production have occurred as a direct result of the use of reproductive technologies that were intended to improve the transfer of genes important for food production [Gerrits et al., Theriogenology 63:283-299, 2005]. While some technologies focused on the efficiency of gene transfer, others addressed fertility and labor issues. Among livestock species, pig reproductive efficiency appears to have achieved exceptionally high rates of performance (PigCHAMP 2011) [Benchmark 2011, Ames, IA, 12-16]. From the maternal side, this includes pigs born per litter, farrowing rate, as well as litters per sow per year. On the male side, boar fertility, sperm production, and sows served per sire have improved as well [Knox et al., Theriogenology, 70:1202-1208, 2008]. These shifts in the efficiency of swine fertility have resulted in the modern pig as one of the most efficient livestock species for global food production. These reproductive changes have predominantly occurred in developed countries, but data suggests transfer and adoption of these in developing countries as well (FAO STAT 2009; FAS 2006) [World pig meat production: food and agriculture organization of the United Nations, 2009; FAS, 2006) Worldwide Pork Production, 2006]. Technological advancements in swine reproduction have had profound effects on industry structure, production, efficiency, quality, and profitability. In

  9. The “Criollo Negro de la Costa Ecuatoriana” pigs: effect of sex and rearing system on performance, carcass and meat traits

    Energy Technology Data Exchange (ETDEWEB)

    González, M.A.; Peláez, F.R.; Martínez, A.L.; Avilés, C.; Peña, F.

    2016-11-01

    The objective of this study was to evaluate the effect of rearing system (confinement, C vs semi-confinement, SC) and sex (barrows vs females) on growth performance, carcass traits and meat quality of “Criollo negro de la costa ecuatoriana” pigs. A total of 32 pigs, 16 barrows and 16 gilts were used. The average daily gain (0.30 kg/day vs 0.22 kg/day), slaughter weight (51.4 kg vs 43.1 kg), morphometric parameters and weights for the most valuable meat cuts were higher in C pigs than in SC pigs, whereas hot and cold carcass yields were higher in SC pigs than in C pigs. The loin and sirloin percentages from C pigs were lower and higher compared with those SC pigs, respectively. Carcass of C pigs showed higher percentage of fat and lower percentage of lean and bone that SC pigs. The rearing system had scarce effects on meat quality traits and mineral composition. Meat from C pigs showed lower scores for color, brightness, and unctuousness, and higher for metallist taste, juiciness and persistence of flavor than SC pigs. Few sensory attributes of cooking chops were affected by sex. Metallist taste was higher in barrows, while brightness and lard flavor were higher in females. It can be concluded that the rearing system had a significant effect on most of the productive and carcass traits, whereas its effect was limited on the meat traits. Sex had little influence on most of the traits studied. (Author)

  10. Seroprevalence of Toxoplasma gondii infection in domestic pigs reared under different management in systems in Zimbabwe

    DEFF Research Database (Denmark)

    Hove, T.; Lind, Peter; Mukaratirwa, S.

    2005-01-01

    Serum samples from 474 domestic pigs (Sus scrola) from Zimbabwe were tested for anti-Toxoplasma gondii IgG antibodies using the indirect fluorescent antibody test. The results showed that T gondii infection is widespread in Zimbabwean pigs. Seroprevalence was lowest in fattening pigs from large.......51 % was found in the same group of fattening pigs using an indirect IgG enzyme-linked immunosorbent assay at the single serum dilution of 1:400. The serosurvey shows the importance of modern intensive husbandry systems in reducing the prevalences of T gondii infection in domestic pigs....

  11. Foraging behaviour, nutrient intake from pasture and performance of free-range growing pigs in relation to feed CP level in two organic cropping systems.

    Science.gov (United States)

    Jakobsen, M; Kongsted, A G; Hermansen, J E

    2015-12-01

    In organic pig production one of the major challenges is to be able to fulfil amino acid requirements based on organic and locally grown protein feed crops. The pig is an opportunistic omnivore with a unique capacity for foraging above and below the soil surface. It is hypothesized that direct foraging in the range area can pose an important contribution in terms of fulfilling nutrient requirements of growing pigs. Foraging activity, lucerne nutrient intake and pig performance were investigated in 36 growing pigs, foraging on lucerne or grass and fed either a standard organic pelleted feed mixture (HP: high protein) or a grain mixture containing 48% less CP (LP: low protein) compared with the high protein feed mixture, from an average live weight of 58 kg to 90 kg in a complete block design in three replicates. The pigs were fed 80% of energy recommendations and had access to 4 m2 of pasture/pig per day during the 40 days experimental period from September to October 2013. Behavioural observations were carried out 12 times over the entire experimental period. For both crops, LP pigs rooted significantly more compared with HP pigs but the effect of CP level was more pronounced in grass (44% v. 19% of all observations) compared with lucerne (28% v. 16% of all observations). Feed protein level turned out not to have any significant effect on grazing behaviour but pigs foraging on lucerne grazed significantly more than pigs foraging on grass (10% v. 4% of all observations). Daily weight gain and feed conversion ratio were significantly affected by feed protein and forage crop interactions. Compared to HP pigs, LP treated pigs had 33% lower daily weight gain (589 v. 878 g) and 31% poorer feed conversion ratio (3.75 v. 2.59 kg feed/kg weight gain) in grass paddocks, whereas in lucerne paddocks LP pigs only had 18% lower daily weight gain (741 v. 900 g) and a 14% poorer feed conversion ratio (2.95 v. 2.54 kg feed/kg weight gain) compared with HP pigs. LP pigs foraging on

  12. Effect of pig dung fertilizer on zooplankton production

    African Journals Online (AJOL)

    SARAH

    2014-12-29

    Dec 29, 2014 ... zooplankton population, points out copepods dominance which are rotifers and cladocerans predators. Key-words: pig ... Aquaculture development, especially fish culture, is currently an ... zooplanktons were concentrated in a pond using a plankton net of .... dissolved oxygen between the fertilized and the.

  13. Meganucleases Revolutionize the Production of Genetically Engineered Pigs for the Study of Human Diseases.

    Science.gov (United States)

    Redel, Bethany K; Prather, Randall S

    2016-04-01

    Animal models of human diseases are critically necessary for developing an in-depth knowledge of disease development and progression. In addition, animal models are vital to the development of potential treatments or even cures for human diseases. Pigs are exceptional models as their size, physiology, and genetics are closer to that of humans than rodents. In this review, we discuss the use of pigs in human translational research and the evolving technology that has increased the efficiency of genetically engineering pigs. With the emergence of the clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR-associated (Cas) protein 9 system technology, the cost and time it takes to genetically engineer pigs has markedly decreased. We will also discuss the use of another meganuclease, the transcription activator-like effector nucleases , to produce pigs with severe combined immunodeficiency by developing targeted modifications of the recombination activating gene 2 (RAG2).RAG2mutant pigs may become excellent animals to facilitate the development of xenotransplantation, regenerative medicine, and tumor biology. The use of pig biomedical models is vital for furthering the knowledge of, and for treating human, diseases.

  14. Non-genetic variance in pigs: genetic analysis of reproduction and production traits

    NARCIS (Netherlands)

    Sell-Kubiak, E.B.

    2015-01-01

    Abstract Sell-Kubiak, E. (2015). Non-genetic variance in pigs: genetic analysis of reproduction and production traits. PhD thesis, Wageningen University, The Netherlands The main objective of this thesis was to study the origin of random variance in reproduction and production trait

  15. Establishment of serological herd profiles for zoonoses and production diseases in pigs by "meat juice multi-serology".

    Science.gov (United States)

    Meemken, Diana; Tangemann, Anna Helene; Meermeier, Dieter; Gundlach, Susanne; Mischok, Dieter; Greiner, Matthias; Klein, Guenter; Blaha, Thomas

    2014-03-01

    case of Y. enterocolitica and T. gondii the intra-herd seroprevalence values varied even from zero to 100%. This shows that a serological risk categorization of pig herds regarding zoonoses and production diseases is meaningful if used for risk-based decisions in the framework of the new meat inspection concept and as part of the herd health management system. Thus, the development of a cost-efficient, time- and labour-saving test system for simultaneously detecting various antibodies should be the next step for an extensive implementation of the meat juice multi-serology concept.

  16. Assessment of Antimicrobial Treatment Strategies in Pig Production Using Mathematical Models

    DEFF Research Database (Denmark)

    Ahmad, Amais

    The phenomenon of antimicrobial resistance (AMR) is well established, but its persistent increase and alarming proportions threaten the ability of antimicrobials to treat infections. AMR has become a major issue in veterinary antimicrobial use, specifically in food production animals, due...... to the potential consequences for human health. Danish pig production accounted for 76% of the total veterinary use of antimicrobials in 2012 with 79% of pig production used in weaning pigs. Escherichia coli (E. coli) are the predominant bacteria in the gastrointestinal flora of humans and animals, and can serve...... strategies. Dosing factors, along with the in vivo epidemiological parameters, govern the relation between resistance and antimicrobial use. Mathematical modeling and simulation techniques have been used over the past two decades to evaluate the effect of these factors on the development of resistance...

  17. Efficient production of omega-3 fatty acid desaturase (sFat-1)-transgenic pigs by somatic cell nuclear transfer.

    Science.gov (United States)

    Pan, DengKe; Zhang, Li; Zhou, YanRong; Feng, Chong; Long, Chuan; Liu, Xiao; Wan, Rong; Zhang, Jian; Lin, AiXing; Dong, EnQiu; Wang, ShuChen; Xu, HouGang; Chen, HongXing

    2010-04-01

    Omega-3(omega-3) fatty acid desaturase transgenic pigs may improve carcass fatty acid composition. The use of transgenic pigs is also an excellent large animal model for studying the role of omega-3 fatty acids in the prevention and treatment of coronary heart disease and cancer. Transgenic pigs carrying synthesized fatty acid desaturase-1 gene (sFat-1) from Caenorhabditis briggsae by somatic cell nuclear transfer (SCNT) were produced for the first time in China. Porcine fetal fibroblast cells were transfected with a sFat-1 expression cassette by the liposome-mediated method. Transgenic embryos were reconstructed by nuclear transfer of positive cells into enucleated in vitro matured oocytes. A total of 1889 reconstructed embryos were transferred into 10 naturally cycling gilts. Nine early pregnancies were established, 7 of which went to term. Twenty-one piglets were born. The cloning efficiency was 1.1% (born piglets/transferred embryos). The integration of the sFat-1 gene was confirmed in 15 live cloned piglets by PCR and Southern blot except for 2 piglets. Expression of the sFat-1 gene in 12 of 13 piglets was detected with RT-PCR. The data demonstrates that an efficient system for sFat-1 transgenic cloned pigs was developed, which led to the successful production of piglets expressing the sFat-1 gene.

  18. Efficient production of omega-3 fatty acid desaturase (sFat-1)-transgenic pigs by somatic cell nuclear transfer

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Omega-3(ω-3) fatty acid desaturase transgenic pigs may improve carcass fatty acid composition. The use of transgenic pigs is also an excellent large animal model for studying the role of ω-3 fatty acids in the prevention and treatment of coronary heart disease and cancer. Transgenic pigs carrying synthesized fatty acid desaturase-1 gene (sFat-1) from Caenorhabditis briggsae by somatic cell nuclear transfer (SCNT) were produced for the first time in China. Porcine fetal fibroblast cells were transfected with a sFat-1 expression cassette by the liposome-mediated method. Transgenic embryos were reconstructed by nuclear transfer of positive cells into enucleated in vitro matured oocytes. A total of 1889 reconstructed embryos were transferred into 10 naturally cycling gilts. Nine early pregnancies were established, 7 of which went to term. Twenty-one piglets were born. The cloning efficiency was 1.1% (born piglets/transferred embryos). The integration of the sFat-1 gene was confirmed in 15 live cloned piglets by PCR and Southern blot except for 2 piglets. Expression of the sFat-1 gene in 12 of 13 piglets was detected with RT-PCR. The data demonstrates that an efficient system for sFat-1 transgenic cloned pigs was developed, which led to the successful production of piglets expressing the sFat-1 gene.

  19. Association of polymorphisms of Nrampl gene with immune function and production performance of large white pig

    Institute of Scientific and Technical Information of China (English)

    Hongmei Wu; Duxue Cheng; Lixian Wang

    2008-01-01

    The present research was designed to study the association of polymorphism of natural resistance-associated macrophage protein 1 (Nrampl) with some immune function and the production performance in Large White pig. The PCR-RFLP technique was applied to analyze the correlation between the polymorphisms of Nrampl gene and immune function [value of Polymorphonuclear Leukocytes (PMN) obtained by Nitroblue Tetrazolium (NBT) Reduction and effect of Cytotoxin in Monocyte] and production performance in 165 Large White pigs. The results showed that there was one Nde I restriction locus in Large White pig, and both values of PMN by NBT Reduction and effect of Cytotoxin in Monocyte in genotype BB were higher than those in genotype AB (P<0.05). Simultaneously, the weight of 180-day-old pigs with genotype BB was higher than that with genotype AB (.P<0.05). The results indicated that there was a significant correlation between different genotypes of Nrampl gene and Immune function and production performance, and it can be re garded as a candidate gene of disease resistance. All these results provide valuable reference to further studies of pig disease resistance.

  20. Effects of feeding and rearing systems on growth, carcass composition and meat quality in pigs.

    Science.gov (United States)

    Lebret, B

    2008-10-01

    Animal growth performance and quality of pork depend on the interactive effects of pig genotype, rearing conditions, pre-slaughter handling, and carcass and meat processing. This paper focuses on the effects of feeding and rearing systems (feeding level and diet composition, housing, production system, etc.) on growth performance, carcass composition, and eating and technological qualities of pork. The feeding level and protein : energy ratio can be used to manipulate growth rate or composition of weight gain. Restricted feed allowance strongly reduces growth rate and carcass fatness and also intramuscular fat (IMF) level, resulting in decreased meat tenderness or juiciness. Expression of compensatory growth due to restricted followed by ad libitum feeding modifies the composition of weight gain at both carcass and muscle levels, and may improve meat tenderness due to higher in vivo protein turnover. Decreasing the protein : energy ratio of the diet actually increases IMF and improves eating quality, but gives fatter carcasses. In contrast, a progressive reduction in the protein : energy ratio leads to similar carcass composition at slaughter but with higher IMF. Technological meat traits (pH1, pHu, colour, drip loss) are generally not affected by the level or protein : energy in feed. Modification of fatty acid composition and antioxidant level in meat can be obtained through diet supplementations (e.g. vegetable sources with high n-3 fatty acids), thereby improving the nutritional quality of pork. Influences of pig rearing system on animal performance, carcass and meat traits result from interactive effects of housing (floor type, space allowance, ambient temperature, physical activity), feeding level and genotype in specific production systems. Indoor enrichment (more space, straw bedding) generally increases growth rate and carcass fatness, and may improve meat juiciness or flavour through higher IMF. Outdoor rearing and organic production system have various

  1. Antimicrobial Consumption in Medicated Feeds in Vietnamese Pig and Poultry Production.

    Science.gov (United States)

    Van Cuong, Nguyen; Nhung, Nguyen Thi; Nghia, Nguyen Huu; Mai Hoa, Nguyen Thi; Trung, Nguyen Vinh; Thwaites, Guy; Carrique-Mas, Juan

    2016-09-01

    Antimicrobials are extensively used as growth promoters in animal feeds worldwide, but reliable estimates are lacking. We conducted an internet-based survey of commercial chicken and pig feed products officially approved for sale in Vietnam over the period March-June 2015. Information on the antimicrobial contents in feed products, alongside animal production data, was used to estimate in-feed antimicrobial consumption to produce one kilogram of live animal (chicken, pig), as well as to estimate country-wide antimicrobial consumption through animal feeds. A total of 1462 commercial feed formulations were examined. The survey-adjusted estimated antimicrobial contents were 25.7 and 62.3 mg/kg in chicken and pig feeds, respectively. Overall, it was estimated that 77.4 mg [95% CI 48.1-106.8] and 286.6 mg [95% CI 191.6-418.3] of in-feed antimicrobials were used to raise 1 kg of live chicken and pig, respectively. Bacitracin (15.5% feeds), chlortetracycline (11.4%), and enramycin (10.8%) were the most common antimicrobials present in chicken feed formulations, whereas bacitracin (24.8%), chlortetracycline (23.9%), and florfenicol (17.4%) were the most common in pig feed formulations. Overall, 57% of the total quantitative usage consisted of antimicrobials regarded by WHO of importance for human medicine, including amoxicillin, colistin, tetracyclines, neomycin, lincomycin, and bacitracin. These figures confirm a very high magnitude of in-feed consumption of antimicrobials, especially in pig production. Results from this study should encourage further monitoring of antimicrobials used in animal production, and foster discussion about existing policies on inclusion of antimicrobials in animal feed rations.

  2. Farm factors associated with the use of antibiotics in pig production.

    Science.gov (United States)

    van der Fels-Klerx, H J; Puister-Jansen, L F; van Asselt, E D; Burgers, S L G E

    2011-06-01

    The aim of this study was to investigate farm-level economic and technical factors that are associated with the use of antibiotics on pig farms. Identification of such factors, like farm size and net farm result, may help to increase epidemiological knowledge and to specify farm advice and policy making to reduce inappropriate use of antibiotics. The study used over 300 farm-year records collected during 2004 to 2007 from pig farms in the Netherlands. Data included economic and technical factors as well as antibiotic administration. Data were statistically analyzed for factors associated with antibiotic use, both for fattening pig and sow farms (piglets only), separately. The response variable was the average number of daily dosages per average pig year. Statistical analysis was performed on 16 and 19 potential explanatory factors for the fattening pig and sow farms, respectively. The results showed that, both on the fattening pig and sow farms, the average use of antibiotics increased from 2004 to 2006, but decreased during 2007, but the effect of year was not significant (P > 0.05). Use of antibiotics varied between individual farms. Large farm repeatability for the use of antibiotics in the different years was found. Factors associated (P antibiotics included: farm system, number of pigs, and population density in the region of the farm (for sow farms only). As these factors are easy to collect and to register, they can be used to specify farm advice and investigation, as well as for policy making. The majority of the technical and economic factors were not significantly (P > 0.05) related to the on-farm use of antibiotics. Therefore, it is recommended to focus future research on the potential role of socioeconomic factors associated with antibiotic use on pig farms.

  3. Environmental impact of heavy pig production in a sample of Italian farms. A cradle to farm-gate analysis

    Energy Technology Data Exchange (ETDEWEB)

    Pirlo, G., E-mail: giacomo.pirlo@crea.gov.it [Council for Agricultural Research and Economics, Fodder and Dairy Production Research Centre, Via A. Lombardo 11, 26900 Lodi (Italy); Carè, S. [Council for Agricultural Research and Economics, Fodder and Dairy Production Research Centre, Via A. Lombardo 11, 26900 Lodi (Italy); Casa, G. Della; Marchetti, R.; Ponzoni, G.; Faeti, V. [Council for Agricultural Research and Economics, Research Unit for Swine Husbandry, Via Beccastecca 345, San Cesario sul Panaro, 41018 (Italy); Fantin, V.; Masoni, P.; Buttol, P. [ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Via Martiri di Monte Sole 4, 40129 Bologna (Italy); Zerbinatti, L. [Associazione Regionale Allevatori dell' Emilia Romagna, Via Roma 89/2—Loc. Viadagola, 40057 Granarolo dell' Emilia (Italy); Falconi, F. [LCA-lab SRL, Bologna (Italy)

    2016-09-15

    Four breeding piggeries and eight growing-fattening piggeries were analyzed to estimate potential environmental impacts of heavy pig production (> 160 kg of live height at slaughtering). Life Cycle Assessment methodology was adopted in the study, considering a system from breeding phase to growing fattening phase. Environmental impacts of breeding phase and growing-fattening phase were accounted separately and then combined to obtain the impacts of heavy pig production. The functional unit was 1 kg of live weight gain. Impact categories investigated were global warming (GW), acidification (AC), eutrophication (EU), abiotic depletion (AD), and photochemical ozone formation (PO). The total environmental impact of 1 kg of live weight gain was 3.3 kg CO{sub 2}eq, 4.9 E − 2 kg SO{sub 2}eq, 3.1 E − 2 kg PO{sub 4}{sup 3−} eq, 3.7 E − 3 kg Sbeq, 1.7 E − 3 kg C{sub 2}H{sub 4}eq for GW, AC, EU, AD, and PO respectively. Feed production was the main hotspot in all impact categories. Greenhouse gases responsible for GW were mainly CH{sub 4}, N{sub 2}O, and CO{sub 2}. Ammonia was the most important source of AC, sharing about 90%. Nitrate and NH{sub 3} were the main emissions responsible for EU, whereas P and NOx showed minor contributions. Crude oil and natural gas consumption was the main source of AD. A large spectrum of pollutants had a significant impact on PO: they comprised CH{sub 4} from manure fermentation, CO{sub 2} caused by fossil fuel combustion in agricultural operations and industrial processes, ethane and propene emitted during oil extraction and refining, and hexane used in soybean oil extraction. The farm characteristics that best explained the results were fundamentally connected with performance indicators Farms showed a wide variability of results, meaning that there was wide margin for improving the environmental performance of either breeding or growing-fattening farms. The effectiveness of some mitigation measures was evaluated and the results

  4. Consumer choice and suggested price for pork as influenced by its appearance, taste and information concerning country of origin and organic pig production

    DEFF Research Database (Denmark)

    Dransfield, E.; Ngapo, T.M.; Nielsen, Niels Asger

    2005-01-01

    Reactions of consumers to the appearance and taste of pork with and without information concerning outdoor production of pigs were tested in France, Denmark, Sweden and UK. Consumers in all four countries focussed on colour and fatness rather than marbling and drip to make their choice. Almost half...... as pork from pigs 'raised outside' as opposed to 'inside'. There was no difference in the taste of grilled pork from indoor and outdoor production systems but pork labelled 'home produced' or 'outdoor' were more appreciated. Consumers' willingness to pay varied widely and was higher for those consumers...

  5. Fermentation of liquid diets for pigs

    OpenAIRE

    Scholten, R.

    2001-01-01

    In the last 30 years major changes occurred in pig nutrition. With the increasing size of the pig farms, manual feeding was replaced by automatic feeding systems. Most pigs were fed on dry diets, but during last the 15 years a clear tendency towards liquid feeding systems was observed. In the early 80's the use of liquid co-products from the human food industry was introduced into pig nutrition. In the Netherlands, nowadays about 20% of the slaughter pigs and about 10% of the sows is fed a li...

  6. Product Service Systems

    DEFF Research Database (Denmark)

    Departing from Product Development models based on physical artefacts. Moving towards integrated Product Development and System Operations models suited Product/Service-systems......Departing from Product Development models based on physical artefacts. Moving towards integrated Product Development and System Operations models suited Product/Service-systems...

  7. Product Service Systems

    DEFF Research Database (Denmark)

    Departing from Product Development models based on physical artefacts. Moving towards integrated Product Development and System Operations models suited Product/Service-systems......Departing from Product Development models based on physical artefacts. Moving towards integrated Product Development and System Operations models suited Product/Service-systems...

  8. 抗生素对苏淮猪发酵床养殖环境的影响%Effect of Antibiotics on Production Environment of Suhuai Pigs Based on Ferment Bed Culture System

    Institute of Scientific and Technical Information of China (English)

    何鑫; 任正平; 陆冬梅; 何敏; 于传军; 王均顺; 黄瑞华

    2009-01-01

    Twenty Su Huai pigs with average weight of 30.0±5.5kg were selected and allotted into 4 groups in order to study the possiblility of dietary or injected antibiotics in the fermentation bed cultivation system of Suhuai pigs.Healthy pigs were allotted to the control group (CT) or treatment 1 (T1).No dietary or injected antibiotics was used to the pigs in CT.Preventive level of antibiotics were dietary supplemented in T1.Pigs with occasionally short coughs (within 5 times per minute) were allotted into T2 and clinical level of antibiotics were dietary supplemented.Those with continuously coughs were allotted into T3 and clinical level of antibiotics were dietary supplemented and intramuscular injected.The result did not show significant difference of any item between T1 and CT.Items of bed temperature, humidity, fecal decomposition ability and pen smell in T2 and T3 were significantly affected by the application of antibiotics.%选择苏淮猪20头,按照性别相同(母猪)、体重一致(30.0±5.5)kg的原则分为4个组.对照组(CT)为健康猪不添加或注射任何药物,组1(T1)为健康猪群,采用拌料方式,用药量为预防量;组2(T2)为轻微咳嗽猪群,采用拌料方式,用药量为治疗量;组3(T3)为重症连续长咳猪群,采用拌料和肌注两种方式结合治疗.试验期30 d,分别对每组床体进行温湿度测量,及其各组床体对粪便的分解能力和圈舍气味进行评定.结果表明各项指标T1组与对照组差异不显著(P>0.05).T2组发酵床床体中层15 cm和下层30 cm处温度显著低于对照组(P<0.05),垫料表面湿度和内部湿度均显著大于对照组(P<0.01),对粪便的分解能力显著(P<0.05)低于对照组.T3组发酵床床体表层0 cm处(P<0.05)、中层15 cm(P<0.05)和下层30 cm(P<0.01)处温度显著低于对照组,垫料表面湿度和内部湿度均显著大于对照组(P<0.01),对粪便的分解能力显著(P<0.01)低于对照组,且T3组

  9. Review on the consequences of using Improvac TM in modern pig production

    Directory of Open Access Journals (Sweden)

    Anna Katharina Albrecht

    2013-01-01

    Full Text Available In Europe growing concerns regarding animal welfare issues in pig production have forced the pig industry to introduce alternative methods to conventional castration procedures. Besides the raising of entire males and castration in combination with analgesia and anaesthesia the vaccination against GnRF (Gonadotropin-releasing factor seems to be the most promising long-term solution. Immunised male pigs (IM show higher average daily weight gain than surgically castrated males (CM. Additionally the feed intake in IM is lower than in CM and feed conversion ratio is consequently better. Carcass weight, back fat depth and dressing percentage of IM pigs are intermediate between CM and entire males while meat quality seems not to be influenced by castration technique since CM and IM show comparable results. Steroid hormone concentrations in IM decline to very low levels (below detection line after the second administration of the anti-GnRF vaccine and boar taint compounds are reliably metabolized. Pigs which received two injections of the anti-GnRF vaccine reduce their sexual and aggressive behaviour to levels of CM pigs which results in low incidents of injury and carcass damages. Surveys analyzing the consumers’ attitude to vaccination against boar taint reveal that if profound information on the technique is provided, the acceptance of meat from vaccinated animals is even better than the acceptance of meat from pigs castrated under current farm conditions. Furthermore economic analyses reveal that immunisation against GnRF provides a potential for a return on investment since better feed efficiency compensates for the additional costs of drug and labour time.

  10. Genetic relatedness of commensal Escherichia coli from nursery pigs in intensive pig production in Denmark and molecular characterization of genetically different strains

    DEFF Research Database (Denmark)

    Herrero Fresno, Ana; Larsen, Inge; Olsen, John Elmerdahl

    2015-01-01

    AIMS: To determine the genetic relatedness and the presence of virulence and antibiotic resistance genes in commensal Escherichia coli from nursery pigs in Danish intensive production. METHODS AND RESULTS: The genetic diversity of 1000 E. coli strains randomly picked (N = 50 isolates) from cultured...... faecal samples (N = 4 pigs) from five intensive Danish pigs farms was analysed by repetitive extragenic palindromic-PCR (REP-PCR) and 42 unique REP-profiles were detected (similarity commensal E. coli......-producing genes were observed in 20 isolates. CONCLUSIONS: A low genetic diversity was found in commensal gut E. coli from nursery pigs in Denmark. No correlation was observed between REP-profiles, ST-types and resistance/virulence patterns. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study analysing...

  11. Intestinal microbial affects of yeast products on weaned and transport stressed pigs

    Science.gov (United States)

    Study objectives were to determine effects of a commercially available yeast product (XPC, Diamond-V Mills) and stress of transportation on total Enterobacteriaceae, Escherichia coli, coliforms, and Lactobacilli populations in the intestine of weaning pigs. In a RCB design with a 2 x 2 factorial ar...

  12. From pig to pork: methicillin-resistant Staphylococcus aureus in the pork production chain.

    Science.gov (United States)

    Lassok, Birgit; Tenhagen, Bernd-Alois

    2013-06-01

    Methicillin-resistant Staphylococcus aureus (MRSA) is a major global public health concern and could be a food safety issue. Recurrent reports have documented that pig herds are an important reservoir for MRSA, specifically the livestock-associated sequence type 398. The high prevalence of MRSA in pig primary production facilities and the frequent detection of MRSA of the same types in pork and pig meat products raise the question of underlying mechanisms behind the introduction and transmission of MRSA along the pork production chain. A comprehensive review of current literature on the worldwide presence of livestock-associated MRSA in various steps of the pork production chain revealed that the slaughter process plays a decisive role in MRSA transmission from farm to fork. Superficial heat treatments such as scalding and flaming during the slaughter process can significantly reduce the burden of MRSA on the carcasses. However, recontamination with MRSA might occur via surface treating machinery, as a result of fecal contamination at evisceration, or via increased human handling during meat processing. By optimizing processes for carcass decontamination and avoiding recontamination by effective cleaning and personal hygiene management, transmission of MRSA from pig to pork can be minimized.

  13. Production of cloned pigs with targeted attenuation of gene expression.

    Directory of Open Access Journals (Sweden)

    Vilceu Bordignon

    Full Text Available The objective of this study was to demonstrate that RNA interference (RNAi and somatic cell nuclear transfer (SCNT technologies can be used to attenuate the expression of specific genes in tissues of swine, a large animal species. Apolipoprotein E (apoE, a secreted glycoprotein known for its major role in lipid and lipoprotein metabolism and transport, was selected as the target gene for this study. Three synthetic small interfering RNAs (siRNA targeting the porcine apoE mRNA were tested in porcine granulosa cells in primary culture and reduced apoE mRNA abundance ranging from 45-82% compared to control cells. The most effective sequence was selected for cloning into a short hairpin RNA (shRNA expression vector under the control of RNA polymerase III (U6 promoter. Stably transfected fetal porcine fibroblast cells were generated and used to produce embryos with in vitro matured porcine oocytes, which were then transferred into the uterus of surrogate gilts. Seven live and one stillborn piglet were born from three gilts that became pregnant. Integration of the shRNA expression vector into the genome of clone piglets was confirmed by PCR and expression of the GFP transgene linked to the expression vector. Analysis showed that apoE protein levels in the liver and plasma of the clone pigs bearing the shRNA expression vector targeting the apoE mRNA was significantly reduced compared to control pigs cloned from non-transfected fibroblasts of the same cell line. These results demonstrate the feasibility of applying RNAi and SCNT technologies for introducing stable genetic modifications in somatic cells for eventual attenuation of gene expression in vivo in large animal species.

  14. Production of human apolipoprotein(a) transgenic NIBS miniature pigs by somatic cell nuclear transfer.

    Science.gov (United States)

    Shimatsu, Yoshiki; Horii, Wataru; Nunoya, Tetsuo; Iwata, Akira; Fan, Jianglin; Ozawa, Masayuki

    2016-01-01

    Most cases of ischemic heart disease and stroke occur as a result of atherosclerosis. The purpose of this study was to produce a new Nippon Institute for Biological Science (NIBS) miniature pig model by somatic cell nuclear transfer (SCNT) for studying atherosclerosis. The human apolipoprotein(a) (apo(a)) genes were transfected into kidney epithelial cells derived from a male and a female piglet. Male cells were used as donors initially, and 275 embryos were transferred to surrogates. Three offspring were delivered, and the production efficiency was 1.1% (3/275). Serial female cells were injected into 937 enucleated oocytes. Eight offspring were delivered (production efficiency: 0.9%) from surrogates. One male and 2 female transgenic miniature pigs matured well. Lipoprotein(a) was found in the male and one of the female transgenic animals. These results demonstrate successful production of human apo(a) transgenic NIBS miniature pigs by SCNT. Our goal is to establish a human apo(a) transgenic NIBS miniature pig colony for studying atherosclerosis.

  15. Supplementation of Lactobacillus acidophilus fermentation product can attenuate the acute phase response following a lipopolysaccharide challenge in pigs.

    Science.gov (United States)

    This study was designed to determine if feeding a Lactobacillus acidophilus fermentation product to weaned pigs would reduce stress and acute phase responses (APR) following a lipopolysaccharide (LPS) challenge. Pigs (n=30; 6.4±0.1 kilograms body weight) were housed individually in pens with ad libi...

  16. Thai pigs and cattle production, genetic diversity of livestock and strategies for preserving animal genetic resources

    Directory of Open Access Journals (Sweden)

    Kesinee Gatphayak

    2013-03-01

    Full Text Available This paper reviews the current situation of livestock production in Thailand, genetic diversity and evaluation, as well as management strategies for animal genetic resources focusing on pigs and cattle. Sustainable conservation of indigenous livestock as a genetic resource and vital components within the agricultural biodiversity domain is a great challenge as well as an asset for the future development of livestock production in Thailand.

  17. Saccharomyces cerevisiae Live Cells Decreased In vitro Methane Production in Intestinal Content of Pigs.

    Science.gov (United States)

    Gong, Y L; Liao, X D; Liang, J B; Jahromi, M F; Wang, H; Cao, Z; Wu, Y B

    2013-06-01

    An in vitro gas production technique was used in this study to elucidate the effect of two strains of active live yeast on methane (CH4) production in the large intestinal content of pigs to provide an insight to whether active live yeast could suppress CH4 production in the hindgut of pigs. Treatments used in this study include blank (no substrate and no live yeast cells), control (no live yeast cells) and yeast (YST) supplementation groups (supplemented with live yeast cells, YST1 or YST2). The yeast cultures contained 1.8×10(10) cells per g, which were added at the rates of 0.2 mg and 0.4 mg per ml of the fermented inoculum. Large intestinal contents were collected from 2 Duroc×Landrace×Yorkshire pigs, mixed with a phosphate buffer (1:2), and incubated anaerobically at 39°C for 24 h using 500 mg substrate (dry matter (DM) basis). Total gas and CH4 production decreased (pyeast. The methane production reduction potential (MRP) was calculated by assuming net methane concentration for the control as 100%. The MRP of yeast 2 was more than 25%. Compared with the control group, in vitro DM digestibility (IVDMD) and total volatile fatty acids (VFA) concentration increased (pyeast supplementation. Quantity of methanogenic archaea per milliliter of inoculum decreased (pyeast supplementation after 24 h of incubation. Our results suggest that live yeast cells suppressed in vitro CH4 production when inoculated into the large intestinal contents of pigs and shifted the fermentation pattern to favor propionate production together with an increased population of acetogenic bacteria, both of which serve as a competitive pathway for the available H2 resulting in the reduction of methanogenic archaea.

  18. A simplified approach to calculate slurry production of growing pigs at farm level

    Directory of Open Access Journals (Sweden)

    Franco Tagliapietra

    2010-01-01

    Full Text Available A simplified approach to predict the amount of slurry produced by growing pigs at farm level is proposed. The inputs are initial (LWi and final (LWf live weights, production (t and empty (empty periods, feed consumption (FC, dry matter (DMD, N digestibilities and farm water consumption per pig (FWC. Estimates of the amount of water required (or arisen per kg of feed for the various physiological functions were estimated by running a published mathematical model using data representing the ordinary conditions of rearing. Water excretion was estimated in two ways depending on: 1 free access (ad lib to water; 2 restricted access (forced. In the first case, the proportion of water consumed (wiad lib and those excreted with the urine (wuad lib and the faeces (wfec were quantified to be 2.9, 1.72 and 0.33 kg per kg of feed, respectively. From the urinary excretions of N and minerals, obtained as the difference between the digestible nutrient intakes and the retentions, the model predicted a urinary DM content of 2.1% (by weight. In the second case, for pigs receiving drinking water in forced ratio with the feed (wiforced, the urinary production was calculated as wuforced=(wiforced+wf+wo-(wd+ws+wg+wfec+we, where wf=water content in feed (0.12 kg/kg, wo=water arising from nutrient oxidation (0.25 kg/kg, wd=water required for digestion (0.08 kg/kg, ws=water demand for protein and lipid synthesis (0.06 kg/kg, wg=water retained in body tissues (0.14 kg/kg and we=water lost through evaporation (0.96 kg/kg. Estimates of fresh slurry production (faeces+urine were regressed against the values resulting from empirical literature equations and referred to pigs fed water:feed ratios of 2.5:1, 2.9:1 and 4:1. The resulting regression (R2=0.97, with a slope close to unity (1.05, indicated that the approach can be extended to predict the farm fresh slurry production with pigs having free access to water or kept on different water:feed ratios. In agreement with

  19. Resistance to colistin: what is the fate for this antibiotic in pig production?

    Science.gov (United States)

    Rhouma, Mohamed; Beaudry, Francis; Letellier, Ann

    2016-08-01

    Colistin, a cationic polypeptide antibiotic, has reappeared in human medicine as a last-line treatment option for multidrug-resistant Gram-negative bacteria (MDR-GNB). Colistin is widely used in veterinary medicine for the treatment of gastrointestinal infections caused by Enterobacteriaceae. GNB resistant to colistin owing to chromosomal mutations have already been reported both in human and veterinary medicine, however several recent studies have just identified a plasmid-mediated mcr-1 gene encoding for colistin resistance in Escherichia coli colistin resistance. The discovery of a non-chromosomal mechanism of colistin resistance in E. coli has led to strong reactions in the scientific community and to concern among physicians and veterinarians. Colistin use in food animals and particularly in pig production has been singled out as responsible for the emergence of colistin resistance. The present review will focus mainly on the possible link between colistin use in pigs and the spread of colistin resistance in Enterobacteriaceae. First we demonstrate a possible link between Enterobacteriaceae resistance emergence and oral colistin pharmacokinetics/pharmacodynamics and its administration modalities in pigs. We then discuss the potential impact of colistin use in pigs on public health with respect to resistance. We believe that colistin use in pig production should be re-evaluated and its dosing and usage optimised. Moreover, the search for competitive alternatives to using colistin with swine is of paramount importance to preserve the effectiveness of this antibiotic for the treatment of MDR-GNB infections in human medicine. Copyright © 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

  20. Production of Ban miniature pig embryos by in vitro fertilization: a comparative study with Landrace.

    Science.gov (United States)

    Nguyen, Bui Xuan; Kikuchi, Kazuhiro; Uoc, Nguyen Thi; Dang-Nguyen, Thanh Quang; Linh, Nguyen Viet; Men, Nguyen Thi; Nguyen, Trung Thanh; Nagai, Takashi

    2015-05-01

    Ban is an endangered miniature pig breed in Vietnam. This study aimed to set up an in vitro embryo production (IVP) system for this breed. Ban's epididymal sperm concentration (1240 ± 35 × 10(6) /mL) was lower (P Landrace (4160 ± 42 × 10(6) /mL). However, sperm characteristics before and after freezing in Ban and Landrace were similar. The numbers of follicles with diameter larger than 2 mm per ovary in Ban females treated with equine chorionic gonadotropin and human chorionic gonadotropin (27.1 ± 1.3) were higher (P Landrace (12.9 ± 2.0) and in non-hormone stimulated Ban (no > 2 mm follicles). After in vitro maturation, the percentages of oocytes with expanded cumulus cells and the first polar body (matured oocytes) were not different among Ban, hormone-stimulated Ban and Landrace. The percentages of two-cell embryos and morulae derived from oocytes collected from three sources did not differ. However, the rate of blastocysts derived from oocytes in non-stimulated Ban (4.0 ± 3.8%) was lower (P Landrace (15.3 ± 1.8%). In conclusion, an effective IVP system for good quality embryos in Ban, that is essential for genetic conservation of this breed, was established.

  1. Review of wallowing in pigs: implications for animal welfare

    NARCIS (Netherlands)

    Bracke, M.B.M.; Spoolder, H.A.M.

    2011-01-01

    Most modern production systems, especially in temperate climates, do not offer wallowing facilities to pigs and, to date, this has neither generated much concern in welfare science nor public debate on pig welfare. Nevertheless, wallowing is a natural behaviour of pigs which may be important to them

  2. Review of wallowing in pigs: implications for animal welfare

    NARCIS (Netherlands)

    Bracke, M.B.M.; Spoolder, H.A.M.

    2011-01-01

    Most modern production systems, especially in temperate climates, do not offer wallowing facilities to pigs and, to date, this has neither generated much concern in welfare science nor public debate on pig welfare. Nevertheless, wallowing is a natural behaviour of pigs which may be important to

  3. Heat production and quantitative oxidation of nutrients by physical activity in pigs

    DEFF Research Database (Denmark)

    Jakobsen, K; Chwalibog, André; Henckel, S

    1994-01-01

    Two groups of pigs weighing 90 (Expt A) or 80 (Expt B) kg walked on a horizontal moving rubber belt for a distance of 315 m at a speed of 25.6 +/- 0.38 and 28.8 +/- 0.35 m/min respectively for 10 min in an open-air-circuit respiration unit. From measurements of VO2 and VCO2, heat production...

  4. Possibilities and limitations of protein supply in organic poultry and pig production

    OpenAIRE

    2005-01-01

    It is one of the general recommendations in animal nutrition that the diet should be formulated according to the specific requirements of animals at the various stages of their development. To which degree the farmer can adapt the nutrient supply to the specific requirements of the animals depends primarily on the production goal and on the availability of nutrient resources. This report gives a general introduction to the present situation for dietary protein supply to poultry and pig produc...

  5. Product Configuration Systems and Productivity

    DEFF Research Database (Denmark)

    Pedersen, Jørgen Lindgaard; Edwards, Kasper

    2004-01-01

    Twelve companies have been interviewed with the purpose to get information about technical, economic and organisational matters in respect of Product Configuration Systems (PCS).Combinations of qualitative interviews and quantitative scoring have been used in ranking expected and realized results...... from implementing PCS. The three highest aggregated scoring expected benefits are: 1)improved quality in specifications, 2)lower turnaround time, 3)less resource intensity.......Twelve companies have been interviewed with the purpose to get information about technical, economic and organisational matters in respect of Product Configuration Systems (PCS).Combinations of qualitative interviews and quantitative scoring have been used in ranking expected and realized results...

  6. Glutamate-induced production of nitric oxide in guinea pig vestibular sensory cells.

    Science.gov (United States)

    Takumida, M; Anniko, M

    2000-06-01

    Glutamate-induced production of nitric oxide (NO) in the vestibular organ of the guinea pig was investigated using the new fluorescence indicator, DAF-2DA, for direct detection of NO. Utricular maculae and isolated vestibular sensory cells were examined to locate NO production sites. The fluorescence intensity of the sensory cells was augmented by stimulation with glutamate, NMDA and AMPA. This is the first direct evidence of NO production in the vestibular end organs. NO may play an important role in the glutamate-induced ototoxicity and also be involved in disease of the inner ear.

  7. Enhanced methane production from pig slurry with pulsed electric field pre-treatment.

    Science.gov (United States)

    Safavi, Seyedeh Masoumeh; Unnthorsson, Runar

    2017-03-08

    Intensive amount of manure produced in pig breeding sectors represents negative impact on the environment and requires optimal management. Anaerobic digestion as a well-known manure management process was optimized in this experimental study by pulsed electric field (PEF) pre-treatment. The effect of PEF on methane production was investigated at three different intensities (15, 30 and 50 kWh/m(3)). The results indicate that the methane production and chemical oxygen demand (COD) removal was improved by continuous escalation of applied intensity, up to 50 kWh/m(3). In comparison with untreated slurry, methane production and COD removal were increased up to 58% and 44%, respectively.

  8. Pig production in Kenya, forms an important component of the ...

    African Journals Online (AJOL)

    user

    1 Animal Genetic Resources Group, National Animal Husbandry Research Centre ..... notwithstanding conditions of small herd sizes and inconsistent recording. ... The study was supported by the Kenya Agricultural Productivity Project (KAPP).

  9. Red cell amino acid transport. Evidence for the presence of system Gly in guinea pig reticulocytes.

    Science.gov (United States)

    Fincham, D A; Willis, J S; Young, J D

    1984-10-17

    Guinea pig reticulocytes are shown to possess an Na+-dependent glycine transporter which also requires Cl- for activity. Glycine transport by this route is saturable (apparent Km 98 microM; Vmax 24 mumol/g Hb per h) and inhibited by sarcosine. The properties of this carrier closely resemble those of System Gly previously demonstrated in pigeon and human erythrocytes. In contrast, no System Gly activity was detected in mature guinea pig erythrocytes.

  10. Multivariate prediction of odor from pig production based on in-situ measurement of odorants

    Science.gov (United States)

    Hansen, Michael J.; Jonassen, Kristoffer E. N.; Løkke, Mette Marie; Adamsen, Anders Peter S.; Feilberg, Anders

    2016-06-01

    The aim of the present study was to estimate a prediction model for odor from pig production facilities based on measurements of odorants by Proton-Transfer-Reaction Mass spectrometry (PTR-MS). Odor measurements were performed at four different pig production facilities with and without odor abatement technologies using a newly developed mobile odor laboratory equipped with a PTR-MS for measuring odorants and an olfactometer for measuring the odor concentration by human panelists. A total of 115 odor measurements were carried out in the mobile laboratory and simultaneously air samples were collected in Nalophan bags and analyzed at accredited laboratories after 24 h. The dataset was divided into a calibration dataset containing 94 samples and a validation dataset containing 21 samples. The prediction model based on the measurements in the mobile laboratory was able to explain 74% of the variation in the odor concentration based on odorants, whereas the prediction models based on odor measurements with bag samples explained only 46-57%. This study is the first application of direct field olfactometry to livestock odor and emphasizes the importance of avoiding any bias from sample storage in studies of odor-odorant relationships. Application of the model on the validation dataset gave a high correlation between predicted and measured odor concentration (R2 = 0.77). Significant odorants in the prediction models include phenols and indoles. In conclusion, measurements of odorants on-site in pig production facilities is an alternative to dynamic olfactometry that can be applied for measuring odor from pig houses and the effects of odor abatement technologies.

  11. Improved biogas production from rice straw by co-digestion with kitchen waste and pig manure

    Energy Technology Data Exchange (ETDEWEB)

    Ye, Jingqing [Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640 (China); School of Environmental Sciences and Engineering, Sun Yat-sen University, Guangzhou 510275 (China); Li, Dong; Sun, Yongming [Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640 (China); Wang, Guohui [School of Environmental Sciences and Engineering, Sun Yat-sen University, Guangzhou 510275 (China); Yuan, Zhenhong, E-mail: yuanzh@ms.giec.ac.cn [Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640 (China); Zhen, Feng; Wang, Yao [Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640 (China)

    2013-12-15

    Highlights: • Biogas production was enhanced by co-digestion of rice straw with other materials. • The optimal ratio of kitchen waste, pig manure and rice straw is 0.4:1.6:1. • The maximum biogas yield of 674.4 L/kg VS was obtained. • VFA inhibition occurred when kitchen waste content was more than 26%. • The dominant VFA were propionate and acetate in successful reactors. - Abstract: In order to investigate the effect of feedstock ratios in biogas production, anaerobic co-digestions of rice straw with kitchen waste and pig manure were carried out. A series of single-stage batch mesophilic (37 ± 1 °C) anaerobic digestions were performed at a substrate concentration of 54 g/L based on volatile solids (VS). The results showed that the optimal ratio of kitchen waste, pig manure, and rice straw was 0.4:1.6:1, for which the C/N ratio was 21.7. The methane content was 45.9–70.0% and rate of VS reduction was 55.8%. The biogas yield of 674.4 L/kg VS was higher than that of the digestion of rice straw or pig manure alone by 71.67% and 10.41%, respectively. Inhibition of biogas production by volatile fatty acids (VFA) occurred when the addition of kitchen waste was greater than 26%. The VFA analysis showed that, in the reactors that successfully produced biogas, the dominant intermediate metabolites were propionate and acetate, while they were lactic acid, acetate, and propionate in the others.

  12. Applications of Systems Genetics and Biology for Obesity Using Pig Models

    DEFF Research Database (Denmark)

    Kogelman, Lisette J. A.; Kadarmideen, Haja N.

    2016-01-01

    In many biomedical research areas, animals have been used as a model to increase the understanding of molecular mechanisms involved in human diseases. One of those areas is human obesity, where porcine models are increasingly used. The pig shows genetic and physiological features that are very...... similar to humans and have shown to be an excellent model for human obesity. Using pig populations, many genetic studies have been performed to unravel the genetic architecture of human obesity. Most of them are pinpointing toward single genes, but more and more studies focus on a systems genetics...... approach, a branch of systems biology. In this chapter, we will describe the state of the art of genetic studies on human obesity, using pig populations. We will describe the features of using the pig as a model for human obesity and briefly discuss the genetics of obesity, and we will focus on systems...

  13. The biosecurity status and its associations with production and management characteristics in farrow-to-finish pig herds.

    Science.gov (United States)

    Postma, M; Backhans, A; Collineau, L; Loesken, S; Sjölund, M; Belloc, C; Emanuelson, U; Grosse Beilage, E; Stärk, K D C; Dewulf, J

    2016-03-01

    Disease prevention through biosecurity measures is believed to be an important factor for improvement of the overall health status in animal production. This study aimed at assessing the levels of implementation of biosecurity measures in pig production in four European Union (EU) countries and to describe possible associations between the biosecurity level and farm and production characteristics. A cross-sectional study was conducted in 232 farrow-to-finish pig herds in Belgium, France, Germany and Sweden between December 2012 and December 2013. The biosecurity status in each of these herds was described and quantified by using the risk-based scoring tool Biocheck.UGentTM (www.biocheck.ugent.be). Production and management characteristics, obtained from the herd management system and by interviewing the farmer, were analysed for their association with the biosecurity level. A causal path was designed to study statistical associations. The results showed that there was substantial room for improvement in the biosecurity status on many pig farms. Significant differences (P<0.01) both in internal and external biosecurity levels were observed between countries. The external biosecurity status, combining all measures taken to prevent disease introduction into the herd, was highest in Germany and lowest in France. The internal biosecurity status, combining all measures taken to prevent within herd disease transmission, was highest in Sweden and lowest, with a large variation, in Belgium. External biosecurity scores were in general higher compared to internal biosecurity scores. The number of pathogens vaccinated against was significantly associated with internal biosecurity status, suggesting an overall more preventive approach towards the risk of disease transmission. A higher external biosecurity was associated with more weaned piglets per sow per year. Furthermore also the weaning age and the mortality till weaning were highly associated with the number of weaned

  14. Morphological traits, reproductive and productive performances of Casertana pigs reared outdoors

    Directory of Open Access Journals (Sweden)

    Donato Matassino

    2010-01-01

    Full Text Available Casertana is an Italian autochthonous pig breed. In the past, the breed was common in Campania and the surrounding area in South Italy because it was valued for its productive performance, particularly regarding the fattening tendency, but in recent decades, autochthonous swine have been replaced by cosmopolitan lean-type pigs and the Casertana breed also experienced a dramatic reduction in numbers. Concern over the preservation of genetic resources and the increased demand for regional food suggest a new way of maintaining the breed. Unfortunately, few recent data on the morpho- logical as well as on the productive traits are available. To ascertain the characteristics of the Casertana breed a pro- ductive trial was performed by comparing Casertana and Large White pure breed and their cross. Sixteen females and 6 males of pure breed Casertana and 18 females and 2 males of pure breed Italian Large White were mated twice to pro- duce Casertana, Large White and Casertana x Large White piglets. The average number of piglets born was significant- ly lower in Casertana gilts, but no difference in the number of weaned piglets among genetic types was observed. Sixty- eight animals of the three genetic types were reared outdoors and received two distinct diets differing in energy content. Pigs were weighted individually every month to calculate average daily gain. Morphological traits of Casertana pigs were also recorded. A total of 54 animals (21 Casertana at 151 kg live weight, 15 crossbreed at 157 kg live weight and 18 Large White at 179 kg live weight were slaughtered and data on carcasses were collected. Average daily weight gain differed markedly (P< 0.05 among genetic types (450; 552; 695 g/d, respectively for Casertana; Casertana x Large White; Large White and consequently the slaughter weight also differed. Casertana showed higher dressing percentages that the Italian Large White (P< 0.05. Backfat thickness was also clearly affected by genetic

  15. The effect of bovine colostrum products on intestinal dysfunction and inflammation in a preterm pig model of necrotizing enterocolitis

    DEFF Research Database (Denmark)

    Støy, Ann Cathrine Findal

    was used to investigate the effect of BC products on intestinal structure, digestive and absorptive functions, microbiota, plasma and tissue proteins and tissue gene expression levels of inflammatory markers. In Study I, the aim was to investigate if BC could correct intestinal dysfunction and reduce...... formula. All three BC products maintained trophic and anti-inflammatory effects on the immature pig intestine. A simple and standardized system was required to investigate the effects of milk formula versus BC on intestinal epithelial cells. In Study III, the IPEC-J2 cell line was evaluated as an in vitro...... formula or colostrum were included for comparison. This study showed that careful considerations must be made prior to gene expression analysis of diet-induced responses in IPEC-J2 cells as a system for the premature intestine, since no dietinduced effects in the IPEC-J2 cells were detected. C...

  16. Improved biogas production from rice straw by co-digestion with kitchen waste and pig manure.

    Science.gov (United States)

    Ye, Jingqing; Li, Dong; Sun, Yongming; Wang, Guohui; Yuan, Zhenhong; Zhen, Feng; Wang, Yao

    2013-12-01

    In order to investigate the effect of feedstock ratios in biogas production, anaerobic co-digestions of rice straw with kitchen waste and pig manure were carried out. A series of single-stage batch mesophilic (37±1 °C) anaerobic digestions were performed at a substrate concentration of 54 g/L based on volatile solids (VS). The results showed that the optimal ratio of kitchen waste, pig manure, and rice straw was 0.4:1.6:1, for which the C/N ratio was 21.7. The methane content was 45.9-70.0% and rate of VS reduction was 55.8%. The biogas yield of 674.4 L/kg VS was higher than that of the digestion of rice straw or pig manure alone by 71.67% and 10.41%, respectively. Inhibition of biogas production by volatile fatty acids (VFA) occurred when the addition of kitchen waste was greater than 26%. The VFA analysis showed that, in the reactors that successfully produced biogas, the dominant intermediate metabolites were propionate and acetate, while they were lactic acid, acetate, and propionate in the others.

  17. Spatiotemporal trends in Canadian domestic wild boar production and habitat predict wild pig distribution

    DEFF Research Database (Denmark)

    Michel, Nicole; Laforge, Michel; van Beest, Floris

    2017-01-01

    improved the fit and predictive ability of the habitat-based model, and the number of boar farms in adjacent rural municipalities had a relative variable importance of 0.84. Our results support the propagule pressure hypothesis, which states that establishment success is linked to source dynamics. Although......Understanding source dynamics of invasive species is crucial to their management. Free-ranging wild pigs (Sus scrofa) have caused considerable ecological and agricultural damage throughout their global range, including Canada. Objectives were to assess the spatial and temporal patterns in domestic...... wild boar and test the propagule pressure hypothesis to improve predictive ability of an existing habitat-based model of wild pigs. We reviewed spatiotemporal patterns in domestic wild boar production across ten Canadian provinces during 1991–2011 and evaluated the ability of wild boar farm...

  18. [Influence of personal attitude of the manager on antibiotic use in pig production].

    Science.gov (United States)

    Malik, J; Kaufmann, G; Hirsiger, P; Kümmerlen, D; Arnold, C; Spring, P; Sidler, X

    2015-12-01

    The attitude as well as the expertise of a person affect the behavior and actions in daily life. To investigate the influence of attitude and knowledge of pig producers on the use of antibiotics in farms, 220 Swiss pig producers were questioned on health awareness, attitude towards sustainable production, risk behavior, intrinsic motivation and knowledge about antibiotics and resistance development. In addition, the strategy of antibiotic use (therapeutic or prophylactic) and the business practice (single or group therapy) for the amount of antibiotics on one hand and for the risk of antibiotic resistance development on the other hand, were determined in a personal interview. Farmers using antibiotics only therapeutically had a better business practice. A direct link between the personal attitude and the antibiotic use or a higher risk of development of antibiotic resistance was not found in this investigation.

  19. The productivity of various pure breed and cross breed of imported pigs during lactation period

    Directory of Open Access Journals (Sweden)

    Djamuara Aritonang

    2001-03-01

    Full Text Available A study has been conducted to evaluate the productivity of pure breeds and cross breeds of imported pigs. Sixteen strains of pigs with five best sows were selected based on litter size and birth weight on the second parity. The results of the study showed that the pure breed performance was better than the cross breeds, as shown by the litter size, birth and weaned weight, less mortality, better mothering ability and better feed efficiency. Between pure breed, the Landrace and Yorkshire were better than other sows and between the cross breed, the LH and YL performed better than other crossbreeds. It is recommended that the pure breed especially Landrace and Yorkshire and LH and YL of cross breed are better than others

  20. EFFECT OF SELENIUM SUPPLEMENTATION ON PIG PRODUCTION PROPERTIES, ANTIOXIDANT STATUS AND MEAT QUALITY

    Directory of Open Access Journals (Sweden)

    Tomislav Šperanda

    2013-12-01

    Full Text Available Food containing functional ingredients to achieve a positive effect on health and reducing the risk of infection is increasing production. It is also very important to improve the quality of pork with respect to change the genetic makeup of pigs that raised leanness, being inversely correlated with the quality of meat. This study monitored the effectiveness of addition of higher doses of organic selenium in the diet of fattening pigs in relation to their growth, immunity, antioxidant power, the quality of meat and possibility of meat enriching with selenium. The experiment was carried out on 100 pigs (crossbred WJxSLxP of both sexes, from 28 kg to 98 kg body weight during a period of 98 days. Piglets fed the finished feed mixture for fattening up to 60 kg (ST-1 and a mixture for fattening up to 100 kg (DM-2 in addition, by the groups as follows: K-0. 3 mg/kg organic selenium, P1-0.5 mg/kg inorganic selenium, P2-0.5 mg/kg organic selenium, P3-0.5 mg/ kg organic selenium +0.2% zeolite clinoptilolite treated vibrotehnology and P4-gradual increase in selenium so that the concentration of the last month was 0.7 mg/ kg diet of organic selenium. All groups of pigs fed high concentration of organic selenium had a higher proportion of lymphocytes, especially CD4 T lymphocytes. Glutathione peroxidase activity was higher in all groups fed elevated selenium levels and significantly higher in the P3 and P4 groups 71st and 98th days of the trials. Glutathione reductase was significantly higher in the P3 and P4 group 98th days compared to the control. Antioxidant indicators suggested increased antioxidant protection in groups supplemented with 0.5 ppm organic selenium and selenium formulations of the same with the addition of zeolite under stress intensive pig production. No differences were found in the products of lipid peroxidation (TBARS in raw meat or in meat after a week in refrigerator storage. By histological examination statistically higher level of

  1. Local and systemic immune response in pigs during subclinical and clinical swine influenza infection.

    Science.gov (United States)

    Pomorska-Mól, M; Kwit, K; Markowska-Daniel, I; Kowalski, C; Pejsak, Z

    2014-10-01

    Local and systemic immune responses in pigs intranasally (IN) and intratracheally (IT) inoculated with swine influenza virus (SIV) were studied. No clinical signs were observed in IN-inoculated pigs, while IT-inoculated pigs developed typical signs of influenza. Significantly higher titres of specific antibodies and changes of haematological parameters were found only in IT-inoculated pigs. Because positive correlations between viral titre, local cytokine concentration, and lung pathology have been observed, we hypothesise that both viral load and the local secretion of cytokines play a role in the induction of lung lesions. It could be that a higher replication of SIV stimulates immune cells to secrete higher amounts of cytokines. The results of the present study indicate that pathogenesis of SIV is dependent on both, the damage caused to the lung parenchyma directly by virus, and the effects on the cells of the host's immune system.

  2. Product Configuration Systems and Productivity

    DEFF Research Database (Denmark)

    Pedersen, Jørgen Lindgaard; Edwards, Kasper

    2004-01-01

    Twelve companies have been interviewed with the purpose to get information about technical, economic and organisational matters in respect of Product Configuration Systems (PCS).Combinations of qualitative interviews and quantitative scoring have been used in ranking expected and realized results...... from implementing PCS. The three highest aggregated scoring expected benefits are: 1)improved quality in specifications, 2)lower turnaround time, 3)less resource intensity....

  3. Systemic PaO2 oscillations cause mild brain injury in a pig model

    OpenAIRE

    Klein, Klaus U.; Johannes, Amelie; Brückner, Melanie; Thomas, Rainer; Matthews, Stephan; Frauenknecht, Katrin; Leukel, Petra; Mazur, Johanna; Poplawski, Alicia; Muellenbach, Ralf; Sommer, Clemens J.; Thal, Serge C.; Engelhard, Kristin

    2016-01-01

    OBJECTIVE: Systemic PaO2 oscillations occur during cyclic recruitment and derecruitment of atelectasis in acute respiratory failure and might harm brain tissue integrity. DESIGN: Controlled animal study. SETTING: University research laboratory. SUBJECTS: Adult anesthetized pigs. INTERVENTIONS: Pigs were randomized to a control group (anesthesia and extracorporeal circulation for 20 hr with constant PaO2, n = 10) or an oscillation group (anesthesia and extracorporeal circulation for 20 hr with...

  4. Angiotensin receptors and actions in guinea pig enteric nervous system.

    Science.gov (United States)

    Wang, Guo-Du; Wang, Xi-Yu; Hu, Hong-Zhen; Fang, Xiu-Cai; Liu, Sumei; Gao, Na; Xia, Yun; Wood, Jackie D

    2005-09-01

    Actions of ANG II on electrical and synaptic behavior of enteric neurons in the guinea pig small intestine were studied. Exposure to ANG II depolarized the membrane potential and elevated neuronal excitability. The number of responding neurons was small, with responses to ANG II in 32% of submucosal neurons and 25% of myenteric neurons. Hyperpolarizing responses were evoked by ANG II in 45% of the neurons. The hyperpolarizing responses were suppressed by alpha2-noradrenergic receptor antagonists, which suggested that the hyperpolarizing responses reflected stimulation of norepinephrine release from sympathetic neurons. Exposure to ANG II enhanced the amplitude and prolonged the duration of noradrenergic inhibitory postsynaptic potentials and suppressed the amplitude of both fast and slow excitatory postsynaptic potentials. The selective ANG II(1) receptor (AT1R) antagonists, ZD-7115 and losartan, but not a selective AT2R antagonist (PD-123319), suppressed the actions of ANG II. Western blot analysis and RT-PCR confirmed expression of AT1R protein and the mRNA transcript for the AT1R in the enteric nervous system. No expression of AT2R protein or mRNA was found. Immunoreactivity for AT1R was expressed by the majority of neurons in the gastric antrum and small and large intestine. AT1R immunoreactivity was coexpressed with calbindin, choline acetyltransferase, calretinin, neuropeptide Y, and nitric oxide synthase in subpopulations of neurons. The results suggest that formation of ANG II might have paracrine-like actions in the enteric nervous system, which include alterations in neuronal excitability and facilitated release of norepinephrine from sympathetic postganglionic axons. The enhanced presence of norepinephrine is expected to suppress fast and slow excitatory neurotransmission in the enteric microcircuits and to suppress neurogenic mucosal secretion.

  5. An Analysis of Pig Production Efficiency and Regional Differences in China Based on Malmquist-DEA Model and Inter-provincial Panel Data

    Institute of Scientific and Technical Information of China (English)

    Yuanyuan ZHANG; Shimin SUN

    2015-01-01

    Pig production efficiency and its regional differences in China have strategic importance to the transformation of pig production mode and optimization of pig production layout in China. In this paper,Malmquist-DEA model is adopted to analyze the statistical data; TFP and its decomposition indicators of different pig breeding scale among the top ten pig producing provinces are calculated; both horizontal and longitudinal comparisons are made. Results show that among different pig breeding scales,free-range breeding scale is dominant in Hunan,small breeding scale is dominant in Sichuan,medium breeding scale is dominant in Sichuan and Hunan,and large breeding scale is dominant in Sichuan,Shandong and Jiangsu; among the provinces,pig production efficiency varies with breeding scale,and small-scale pig breeding in Sichuan,Guangdong and Hebei,free-range and medium-scale pig breeding in Hunan,medium-scale pig breeding in Henan and Yunnan,largescale pig breeding in the rest of the provinces,have significant efficiency advantage.

  6. Immune system stimulation increases the optimal dietary methionine to methionine plus cysteine ratio in growing pigs.

    Science.gov (United States)

    Litvak, N; Rakhshandeh, A; Htoo, J K; de Lange, C F M

    2013-09-01

    Chronic subclinical levels of disease occur frequently in intensive swine production and compromise nutrient use efficiency. Feeding additional Met plus Cys (M+C) has been implicated in improving the response of the animal to immune system stimulation (ISS) because they can serve as substrates for generating compounds involved in the immune response, such as glutathione and acute phase proteins. A N-balance study was conducted to assess the optimal dietary Met to Met plus Cys ratio (M:M+C) during ISS in 20-kg pigs. Thirty-six pigs were fed 800 g/d of 1 of 5 M+C-limiting diets, containing graded levels of M:M+C (0.42, 0.47, 0.52, 0.57, and 0.62) and supplying 2.5 g/d of M+C. After adaptation, N balances were determined sequentially during a 5-d prechallenge period and 2 ISS periods of 3 and 4 d, respectively. To induce ISS, pigs were injected intramuscularly with repeated and increasing doses of Escherichia coli lipopolysaccharide. Eye temperature and blood profile confirmed effective ISS. During ISS period 1, ISS reduced the mean N balance more severely than ISS period 2 (8.7 ± 0.3 vs. 9.6 ± 0.4 g/d; P < 0.001) and was less than the prechallenge period (10.0 ± 0.2 g/d; P < 0.001). An interactive effect of ISS and diet on N balance was observed (P < 0.001). Based on quadratic-plateau regression analysis, the optimal dietary M:M+C was 0.57 ± 0.03 and 0.59 ± 0.02 for the prechallenge period and ISS period 2, respectively. The optimal dietary M:M+C for ISS period 1 was found to be greater than 0.62, indicating that the optimal M:M+C is greater during ISS. It is suggested that this is the result of preferential use of Met during ISS. In conclusion, ISS results in an increase in the optimal dietary M:M+C in growing pigs.

  7. Nonrenewable energy cost and greenhouse gas emissions of a "pig-biogas-fish" system in China.

    Science.gov (United States)

    Yang, Qing; Wu, Xiaofang; Yang, Haiping; Zhang, Shihong; Chen, Hanping

    2012-01-01

    The purpose of this study is to assess the energy savings and emission reductions of the present rural biogas system in China. The life cycle assessment (LCA) method is used to analyze a "pig-biogas-fish" system in Jingzhou, Hubei Province, China. The nonrenewable energy cost and the greenhouse gas (GHG) emissions of the system, including the pigsty, the biogas digester, and the fishpond, are taken into account. The border definition is standardized because of the utilization of the database in this paper. The results indicate that the nonrenewable energy consumption intensity of the "pig-biogas-fish" system is 0.60 MJ/MJ and the equivalent CO₂ emission intensity is 0.05 kg CO₂-eq/MJ. Compared with the conventional animal husbandry system, the "pig-biogas-fish" system shows high renewability and GHG reduction benefit, which indicates that the system is a scientific and environmentally friendly chain combining energy and ecology.

  8. Development of a genetic tool for product regulation in the diverse British pig breed market

    Directory of Open Access Journals (Sweden)

    Wilkinson Samantha

    2012-11-01

    Full Text Available Abstract Background The application of DNA markers for the identification of biological samples from both human and non-human species is widespread and includes use in food authentication. In the food industry the financial incentive to substituting the true name of a food product with a higher value alternative is driving food fraud. This applies to British pork products where products derived from traditional pig breeds are of premium value. The objective of this study was to develop a genetic assay for regulatory authentication of traditional pig breed-labelled products in the porcine food industry in the United Kingdom. Results The dataset comprised of a comprehensive coverage of breed types present in Britain: 460 individuals from 7 traditional breeds, 5 commercial purebreds, 1 imported European breed and 1 imported Asian breed were genotyped using the PorcineSNP60 beadchip. Following breed-informative SNP selection, assignment power was calculated for increasing SNP panel size. A 96-plex assay created using the most informative SNPs revealed remarkably high genetic differentiation between the British pig breeds, with an average FST of 0.54 and Bayesian clustering analysis also indicated that they were distinct homogenous populations. The posterior probability of assignment of any individual of a presumed origin actually originating from that breed given an alternative breed origin was > 99.5% in 174 out of 182 contrasts, at a test value of log(LR > 0. Validation of the 96-plex assay using independent test samples of known origin was successful; a subsequent survey of market samples revealed a high level of breed label conformity. Conclusion The newly created 96-plex assay using selected markers from the PorcineSNP60 beadchip enables powerful assignment of samples to traditional breed origin and can effectively identify mislabelling, providing a highly effective tool for DNA analysis in food forensics.

  9. Pesticide Product Label System

    Data.gov (United States)

    U.S. Environmental Protection Agency — The Pesticide Product Label System (PPLS) provides a collection of pesticide product labels (Adobe PDF format) that have been approved by EPA under Section 3 of the...

  10. Monitoring the Perturbation of Soil and Groundwater Microbial Communities Due to Pig Production Activities

    KAUST Repository

    Hong, Pei-Ying

    2013-02-08

    This study aimed to determine if biotic contaminants originating from pig production farms are disseminated into soil and groundwater microbial communities. A spatial and temporal sampling of soil and groundwater in proximity to pig production farms was conducted, and quantitative PCR (Q-PCR) was utilized to determine the abundances of tetracycline resistance genes (i.e., tetQ and tetZ) and integrase genes (i.e., intI1 and intI2). We observed that the abundances of tetZ, tetQ, intI1, and intI2 in the soils increased at least 6-fold after manure application, and their abundances remained elevated above the background for up to 16 months. Q-PCR further determined total abundances of up to 5.88 × 109 copies/ng DNA for tetZ, tetQ, intI1, and intI2 in some of the groundwater wells that were situated next to the manure lagoon and in the facility well used to supply water for one of the farms. We further utilized 16S rRNA-based pyrosequencing to assess the microbial communities, and our comparative analyses suggest that most of the soil samples collected before and after manure application did not change significantly, sharing a high Bray-Curtis similarity of 78.5%. In contrast, an increase in Bacteroidetes and sulfur-oxidizing bacterial populations was observed in the groundwaters collected from lagoon-associated groundwater wells. Genera associated with opportunistic human and animal pathogens, such as Acinetobacter, Arcobacter, Yersinia, and Coxiella, were detected in some of the manure-treated soils and affected groundwater wells. Feces-associated bacteria such as Streptococcus, Erysipelothrix, and Bacteroides were detected in the manure, soil, and groundwater ecosystems, suggesting a perturbation of the soil and groundwater environments by invader species from pig production activities.

  11. Comparative performance and microbial community of single-phase and two-phase anaerobic systems co-digesting cassava pulp and pig manure

    DEFF Research Database (Denmark)

    Panichnumsin, P.; Ahring, B.K.; Nopharatana, A.

    2010-01-01

    In this study, we illustrated the performance and microbial community of single- and two-phase systems anaerobically co-digesting cassava pulp and pig manure. The results showed that the volatile solid reduction and biogas productivity of two-phase CSTR were 66 ± 4% and 2000 ± 210 ml l-1 d-1, whi...

  12. Environmental assessment of Ronozyme (R) p5000 CT phytase as an alternative to inorganic phosphate supplementation to pig feed used in intensive pig production

    DEFF Research Database (Denmark)

    Nielsen, Per H.; Wenzel, Henrik

    2007-01-01

    Goal, Scope and Background. Ronozyme (R) P5000 CT is an industrially produced enzyme product (phytase) which is able to degrade naturally occurring phytate in animal feed and release the phytate's content of phosphorus for pig's growth. Ronozyme P5000 CT (hereafter called Ronozyme Phytase) can...... use for enzyme production is small and unimportant unless use of agricultural land is given very large relative weight. Recommendations and Perspectives. Hundreds of enzyme products are commercially available on the market today, each with a range of different applications. There are several...

  13. Design and implementation of pig traceability system based on RFID%基于RFID的生猪溯源系统设计与实现

    Institute of Scientific and Technical Information of China (English)

    周邠林; 赵丽敏; 蒋薇

    2014-01-01

    Pig traceability system is a platform based on RFID ( Internet of things ) , the ultimate goal is to share information and serve the enterprise , consumer and government . Through the application of RFID Internet things , the system can track the in-formation of the supply chain of pork products , provide strong data support for pork product traceability . This paper introduces the background of the pig and pig traceability system as well as the overview and development status of pig traceability system at home and abroad , focuses on the design and implementation of the overall architecture of pig traceability system , describes the concrete application of pig traceability system .%基于 RFID 物联网的生猪溯源系统,是以信息共享为最终目的,面向企业、消费者和政府监管部门的服务平台。通过应用 RFID 物联网,对猪肉产品在供应链各个环节的信息进行跟踪,为猪肉产品的可追溯提供强大的数据支持。介绍了生猪行业背景和生猪溯源系统的概况以及国内外发展现状,重点研究了生猪溯源系统的总体架构以及系统的设计与实现,描述了生猪溯源系统的具体应用。

  14. Effect of an automatic feeding system on growth performance and feeding behaviour of pigs reared outdoors

    Directory of Open Access Journals (Sweden)

    Riccardo Fortina

    2010-01-01

    Full Text Available Nine Mora Romagnola and 10 Large White x Mora Romagnola growing pigs were reared outdoors. In both groups ad libitum feed was provided. Conventional pigs received it twice a day, distributed in two long troughs. Inside the corral of the second group, an automatic station was set up for: feed distribution, pigs weighing, and control by an analog camera. Thus the self-feeders received feed ad libitum individually by the automatic system, divided into small quantities at meal times. During the experiment the analog camera was used over 24 hours each day, to collect pictures of pigs in order to investigate their behaviours. For each picture the day and hour, the number of visible pigs and their behaviours were recorded and a statistical analysis of data, which was expressed as hourly frequencies of behavioural elements, was performed. Moreover to highlight “active” and “passive” behaviours between the groups, two categories “Move” and “Rest” were created grouping some behavioural elements. With regard to performance, conventional pigs reached a higher total weight gain (56.1±2.42 kg vs 46.7±2.42 kg; P=0.0117. But the feed conversion index (FCI of both groups was similar. The self-feeders had consumed less feed than conventional animals. The feeding system seems to influence behaviours. The percentage of time spent in Eating activity differs (P<0.0001 between the self-fed (median 24.6% and conventional pigs (median 10.9%. The resulting more regular eating trend of self-feeders influenced the daily activities distribution. The behavioural category Rest (median: self-feeders 55.0% vs 71.4% conventional pigs was dominant, with conventional pigs becoming more restless, particularly at meal times. This type of feeding competition and aggressive behaviour did not happen in the self-feeders due to the feed distribution system. The self-feeder results showed that pigs eat at the automatic station both day and night. The animals perform on

  15. CHANGES AND PERSPECTIVES OF SLOVENIAN PIG PRODUCTION BEFORE AND AFTER ACCESSION TO THE EU: THE CASE STUDY

    Directory of Open Access Journals (Sweden)

    M. Kastelić

    2007-06-01

    Full Text Available The changes in pig production during the last six to ten years were studied in case of Farme Ihan d. d., the Slovenian pig producer. At the beginning of the period, the company produced 70 to 75 thousand slaughtering pigs per year. At the end of the period the production increased to 150 thousand. As expected, the prices of pigs were reduced during that period. According to the new legislation, the large part of facilities was reconstructed and some environment protection investments were done. The production was improved: the number of weaned pigs per sow per year increased from 19.2 in 1996 to 25.48 in 2006 and reached the Danish average. The percentage of meat was increased in the same period from 52.7 % to 58.6 %. The progress in daily gain and in feed conversion ratio was moderate. To secure the access to the market, the own slaughter facility was bought and reconstructed. For the same reason, the company became shareholder of two meat processing companies. The transition period has not been finished yet. Production results may improve faster than in old EU member countries. Investment cycle has also not been finished yet. More investments, especially in environment protection are needed.

  16. Evaluation of Single Column Trapping/Separation and Chemiluminescence Detection for Measurement of Methanethiol and Dimethyl Sulfide from Pig Production

    Directory of Open Access Journals (Sweden)

    Michael Jørgen Hansen

    2012-01-01

    Full Text Available Reduced sulfur compounds are considered to be important odorants from pig production due to their low odor threshold values and low solubility in slurry. The objective of the present study was to investigate the use of a portable method with a single silica gel column for trapping/separation coupled with chemiluminescence detection (SCTS-CL for measurement of methanethiol and dimethyl sulfide in sample air from pig production. Proton-transfer-reaction mass spectrometry (PTR-MS was used to evaluate the trapping/separation. The silica gel column used for the SCTS-CL efficiently collected hydrogen sulfide, methanethiol and dimethyl sulfide. The measurement of methanethiol by SCTS-CL was clearly interfered by the high concentration of hydrogen sulfide found in pig production, and a removal of hydrogen sulfide was necessary to obtain reliable results. Air samples taken from a facility with growing-finishing pigs were analyzed by SCTS-CL, PTR-MS, and a gas chromatograph with sulfur chemiluminescence detection (GC-SCD to evaluate the SCTS-CL. The difference between the concentrations of methanethiol and dimethyl sulfide measured with SCTS-CL, PTR-MS, and GC-SCD was below 10%. In conclusion, the SCTS-CL is a portable and low-cost alternative to the commercial methods that can be used to measure methanethiol and dimethyl sulfide in sample air from pig production.

  17. Pigs and poultry number adequation for joint biodigesters operation systems; Adequacao de planteis de suinos e de frangos de corte para operacao conjunta de biodigestores

    Energy Technology Data Exchange (ETDEWEB)

    Costa, Laura Vanessa C. da; Lucas Junior, Jorge de [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Botucatu, SP (Brazil). Fac. de Ciencias Agronomicas. Dept. de Engenharia Rural], emails: aurahcosta@fca.unesp.br, jlucas@fcav.unesp.br

    2011-07-01

    Pigs and Poultry production are considered activities with large quantity waste generation. It is evaluated that each pig in complete cycle of production produces approximately 150 to 200 L of wastewater per day. In poultry production is not different, larger quantities of litter are generated, approximately 2,0 t for each thousand head. In Brazil, both activities are very close and are coordinated by large companies integrative. The objective of this work was to establish a case study based on experimental results, evaluating the appropriateness of pigs and poultry number relation, the waste production by activities, the relationship of pigs for chicken, as well as the necessary quantity of biofertilizer to dilute the chicken litter in order to operate biodigesters systems for biogas and biofertilizer production. The results showed a good relation for a piggery with 1000 sows and a shed commercial poultry with 250 thousand heard. The total biogas production, for a 50 days period, showed values of 1,477 m{sup 3} and 85,108 m{sup 3}, respectively. (author)

  18. Implantación del Sistema de Gestión de Calidad y Seguridad en la producción de cerdos con destino farmacéutico Introduction of Quality and Safety Management System in pig's production with pharmaceutical fates

    Directory of Open Access Journals (Sweden)

    Leidys Dolores Jiménez Domínguez

    2010-06-01

    Full Text Available Para obtener una materia prima farmacéutica de origen biológico (pulmones útiles de cerdo, a partir de cerdos jóvenes, con los requisitos de calidad e inocuidad establecidos para ser empleada en la producción de medicamentos (Surfacen, se implantó un modelo de Gestión de Calidad y Seguridad en este proceso productivo, para lo cual se emplearon como materiales de trabajo las normas de las Buenas Prácticas de Producción, las regulaciones farmacéuticas, los requisitos del Sistema de Análisis de Peligros y Control de Puntos Críticos, así como la evaluación de los indicadores productivos y de eficiencia. Los resultados obtenidos están en correspondencia con la información recopilada en el diagnóstico aplicado en la etapa del diseño y se evidenciaron en la aplicación de los manuales del Sistema de Análisis de Peligros y Control de Puntos Críticos, los procedimientos normalizativos de operación e instructivos y los registros de las actividades. Se concluye que el sistema implantado permitió capacitar al personal, identificar los puntos críticos de control del proceso, documentar y registrar las actividades, incrementar los controles, así como recopilar datos para medir la trazabilidad de las operaciones y dar respuestas oportunas a las solicitudes de los clientes.To obtain a biological-origin pharmaceutical raw material (pig useful lung from young pigs fulfilling the safety and quality requirements to use in drug manufacture (Surfacen®, Quality and Safety Management model was applied in this productive process where work materials used were the Good Practice of Production guidelines, pharmaceutical regulations, requirements of Analysis System of Dangers and Control of Critical Points, as well as the evaluation of productive end efficient indicators. Results obtained corresponding to collected information in diagnosis applied in design stage evidenced in the manual application of Analysis System of Dangers and Control of

  19. Parasitological measures to characterize different livestock production systems

    DEFF Research Database (Denmark)

    Thamsborg, Stig M.; Mejer, H.; Enemark, Heidi

    2013-01-01

    . Several on-farm surveys have indicated that, despite large farm-to-farm variation, levels and diversity of parasite infections may reflect differences in production systems. In developed countries, organic farming (OF) as a certified livestock production system with a given set of standards, has gained...... momentum in several livestock species, mainly ruminants, poultry and pigs. The changes in management which need to be implemented in order to get the OF status, vary with species and local conditions. For example, conventional pig production is highly intensive and indoors in NW Europe, whereas...... OF practices include outdoor production with associated higher risks of parasites. In dairy cattle production, changes are less dramatic and mostly related to self-sufficiency with feedstuffs and increased use of grazing. But in all cases, there is a reduced reliance on external input, including restrictions...

  20. Evaluation of environmental adjustment contract for pig production in Pinhal river sub-basin

    Directory of Open Access Journals (Sweden)

    Magda Regina Mulinari

    2011-12-01

    Full Text Available The objective of this study was to evaluate the efficiency of Environmental Adjustment Contract for pig production (EAC in improving the water quality in Pinhal River sub-basin, located in Concordia, west part of Santa Catarina State. The monitoring of water parameters occurred in eight sites of the river, during three years (2006-2009. To assess whether the EAC was efficient, Brazilian Water Law was used. The average annual concentrations of Total Dissolved Solids (TDS were: 130.2 mg/L, 137.0 mg/L, and 99.8 mg/L. Turbidity showed the same trend of TDS. Concentrations of nitrate and Total Phosphorus (TP decreased from 2006 to 2009; nitrate from 1.81 mg/L NO3-N to 1.54 mg NO3-N; TP from 0.29 mg/L to 0.10 mg/L, respectively. The same trends occurred for Fecal Coliforms and E. coli. These results show that obligations proposed by EAC had potentially improved water quality. These results can help the government, farmers, and society to establish environmentally sound and sustainable programs for pig production.

  1. Characterization of protected designation of origin Italian meat products obtained from heavy pigs fed barley-based diets.

    Science.gov (United States)

    Prandini, A; Sigolo, S; Gallo, A; Faeti, V; Della Casa, G

    2015-09-01

    A study was conducted to evaluate the quality and sensory properties of protected designation of origin (PDO) Parma ham and Piacentina neck obtained from heavy pigs (Italian Duroc × Italian Large White) fed barley-based diets. Four diets were tested: 1) a corn-based diet (control), 2) the control diet with 80% of a normal-amylose hulled barley variety (Cometa), 3) the control diet with 80% of a normal-amylose hulless barley variety (Astartis), and 4) the control diet with 80% of a low-amylose hulless barley variety (Alamo). All the meat products were analyzed for physicochemical and color parameters. The dry-cured hams and necks were also evaluated for sensory properties. The data of physicochemical, color, and sensory parameters were separately analyzed by multivariate factor analysis, and interpretation of each extracted factor was based on specific original variables loading on each one. The meat products obtained from pigs fed the barley-based diets differed from those obtained from the control pigs on the PUFA factors characterized by C18:2-6 and omega-3:omega-6 ratio. In particular, the meat products obtained from pigs fed the barley-based diets had a lower content of C18:2-6 and a higher omega-3:omega-6 ratio ( diets had subcutaneous fat (SC) with a lower iodine number and a higher SFA level compared with those produced from the control pigs ( diets. The hams from barrows differed from those obtained from gilts on the lean properties factor describing properties related to aspect and odor of dry-cured hams. Indeed, the hams from barrows were depreciated compared with the hams from gilts for minor intensity, brightness, and uniformity of the lean, pinkish intermuscular fat and cured odor. In conclusion, barley could be used as a replacement for corn in heavy pig diets for the production of PDO Italian products without negative effects on the physicochemical, color, or sensory characteristics of meat products.

  2. Environmental Assessment of Ronozyme® P5000 CT Phytase as an Alternative to Inorganic Phosphate Supplementation to Pig Feed Used in Intensive Pig Production

    DEFF Research Database (Denmark)

    Nielsen, Per Henning; Wenzel, Henrik

    2007-01-01

    Ronozyme® P5000 CT is an industrially produced enzyme product (phytase) which is able to degrade naturally occurring phytate in animal feed and release the phytate's content of phosphorus for pig's growth. Ronozyme P5000 CT (hereafter called Ronozyme Phytase) can be used as an alternative...... of agricultural land is given very large relative weight. Hundreds of enzyme products are commercially available on the market today, each with a range of different applications. There are several indications that enzymes like Ronozyme Phytase can play an important role in a transition to a more sustainable...

  3. Production of transgenic cloned pigs expressing the far-red fluorescent protein monomeric Plum.

    Science.gov (United States)

    Watanabe, Masahito; Kobayashi, Mirina; Nagaya, Masaki; Matsunari, Hitomi; Nakano, Kazuaki; Maehara, Miki; Hayashida, Gota; Takayanagi, Shuko; Sakai, Rieko; Umeyama, Kazuhiro; Watanabe, Nobuyuki; Onodera, Masafumi; Nagashima, Hiroshi

    2015-01-01

    Monomeric Plum (Plum), a far-red fluorescent protein with photostability and photopermeability, is potentially suitable for in vivo imaging and detection of fluorescence in body tissues. The aim of this study was to generate transgenic cloned pigs exhibiting systemic expression of Plum using somatic cell nuclear transfer (SCNT) technology. Nuclear donor cells for SCNT were obtained by introducing a Plum-expression vector driven by a combination of the cytomegalovirus early enhancer and chicken beta-actin promoter into porcine fetal fibroblasts (PFFs). The cleavage and blastocyst formation rates of reconstructed SCNT embryos were 81.0% (34/42) and 78.6% (33/42), respectively. At 36-37 days of gestation, three fetuses systemically expressing Plum were obtained from one recipient to which 103 SCNT embryos were transferred (3/103, 2.9%). For generation of offspring expressing Plum, rejuvenated PFFs were established from one cloned fetus and used as nuclear donor cells. Four cloned offspring and one stillborn cloned offspring were produced from one recipient to which 117 SCNT embryos were transferred (5/117, 4.3%). All offspring exhibited high levels of Plum fluorescence in blood cells, such as lymphocytes, monocytes and granulocytes. In addition, the skin, heart, kidney, pancreas, liver and spleen also exhibited Plum expression. These observations demonstrated that transfer of the Plum gene did not interfere with the development of porcine SCNT embryos and resulted in the successful generation of transgenic cloned pigs that systemically expressed Plum. This is the first report of the generation and characterization of transgenic cloned pigs expressing the far-red fluorescent protein Plum.

  4. Production of transgenic cloned pigs expressing the far-red fluorescent protein monomeric Plum

    Science.gov (United States)

    WATANABE, Masahito; KOBAYASHI, Mirina; NAGAYA, Masaki; MATSUNARI, Hitomi; NAKANO, Kazuaki; MAEHARA, Miki; HAYASHIDA, Gota; TAKAYANAGI, Shuko; SAKAI, Rieko; UMEYAMA, Kazuhiro; WATANABE, Nobuyuki; ONODERA, Masafumi; NAGASHIMA, Hiroshi

    2015-01-01

    Monomeric Plum (Plum), a far-red fluorescent protein with photostability and photopermeability, is potentially suitable for in vivo imaging and detection of fluorescence in body tissues. The aim of this study was to generate transgenic cloned pigs exhibiting systemic expression of Plum using somatic cell nuclear transfer (SCNT) technology. Nuclear donor cells for SCNT were obtained by introducing a Plum-expression vector driven by a combination of the cytomegalovirus early enhancer and chicken beta-actin promoter into porcine fetal fibroblasts (PFFs). The cleavage and blastocyst formation rates of reconstructed SCNT embryos were 81.0% (34/42) and 78.6% (33/42), respectively. At 36–37 days of gestation, three fetuses systemically expressing Plum were obtained from one recipient to which 103 SCNT embryos were transferred (3/103, 2.9%). For generation of offspring expressing Plum, rejuvenated PFFs were established from one cloned fetus and used as nuclear donor cells. Four cloned offspring and one stillborn cloned offspring were produced from one recipient to which 117 SCNT embryos were transferred (5/117, 4.3%). All offspring exhibited high levels of Plum fluorescence in blood cells, such as lymphocytes, monocytes and granulocytes. In addition, the skin, heart, kidney, pancreas, liver and spleen also exhibited Plum expression. These observations demonstrated that transfer of the Plum gene did not interfere with the development of porcine SCNT embryos and resulted in the successful generation of transgenic cloned pigs that systemically expressed Plum. This is the first report of the generation and characterization of transgenic cloned pigs expressing the far-red fluorescent protein Plum. PMID:25739316

  5. Investigation of the effect of UV-LED exposure conditions on the production of vitamin D in pig skin

    DEFF Research Database (Denmark)

    Barnkob, Line Lundbæk; Argyraki, Aikaterini; Petersen, Paul Michael;

    2016-01-01

    study the exposure conditions with ultraviolet (UV) LEDs were systematically investigated in the wavelength range 280-340 nm for achieving optimal vitamin D bio-fortification in pig skin. A wavelength of 296 nm was found to be optimal for vitamin D3 production. The maximum dose of 20 kJ/m2 produced 3.......5-4 µg vitamin D3/cm2 pig skin. Vitamin D3 produced was independent on the combination of time and intensity of the LED source. The increased UV exposure by UV-LEDs may be readily implemented in existing food production facilities, without major modifications to the process or processing equipment...

  6. Innovativeness in production systems

    DEFF Research Database (Denmark)

    Blichfeldt, Henrik; Knudsen, Mette Præst

    Alignment of market demand and manufacturing capabilities are directly linked to the potential competitive advantage. Pressure for manufacturing companies to customize increase the need for productions systems to handle innovations, especially in SMEs. The paper claims that innovativeness has...... a positive influence of the ability of the manufacturing system to provide specific capabilities that enables the entire production system to positively influence the competitiveness. A comparative case study of three SMEs shows that competitive advantages are obtained through innovative technologies...

  7. Evaluating environmental impacts of contrasting pig farming systems with life cycle assessment

    NARCIS (Netherlands)

    Dourmad, J.Y.; Ryschawy, J.; Trousson, T.; Bonneau, M.; Gonzalez, J.; Houwers, H.W.J.; Hviid, M.; Zimmer, C.; Nguyen, T.L.T.; Morgensen, L.

    2014-01-01

    Environmental impacts of 15 European pig farming systems were evaluated in the European Union Q-PorkChains project using life cycle assessment. One conventional and two non-conventional systems were evaluated from each of the five countries: Denmark, The Netherlands, Spain, France and Germany. The d

  8. Production of Cloned Miniature Pigs Expressing High Levels of Human Apolipoprotein(a in Plasma.

    Directory of Open Access Journals (Sweden)

    Masayuki Ozawa

    Full Text Available High lipoprotein(a [Lp(a] levels are a major risk factor for the development of atherosclerosis. However, because apolipoprotein(a [apo(a], the unique component of Lp(a, is found only in primates and humans, the study of human Lp(a has been hampered due to the lack of appropriate animal models. Using somatic cell nuclear transfer (SCNT techniques, we produced transgenic miniature pigs expressing human apo(a in the plasma. First, we placed the hemagglutinin (HA-tagged cDNA of human apo(a under the control of the β-actin promoter and cytomegalovirus enhancer, and then introduced this construct into kidney epithelial cells. Immunostaining of cells with anti-HA antibody allowed identification of cells stably expressing apo(a; one of the positive clones was used to provide donor cells for SCNT, yielding blastocysts that expressed apo(a. Immunohistochemical analysis of tissue sections and RT-PCR analysis of total RNA from organs of cloned piglet revealed that apo(a is expressed in various tissues/organs including heart, liver, kidney, and intestine. More importantly, a transgenic line exhibited a high level (>400 mg/dL of Lp(a in plasma, and the transgenic apo(a gene was transmitted to the offspring. Thus, we generated a human apo(a-transgenic miniature pig that can be used as a model system to study advanced atherosclerosis related to human disease. The anatomical and physiological similarities between the swine and human cardiovascular systems will make this pig model a valuable source of information on the role of apo(a in the formation of atherosclerosis, as well as the mechanisms underlying vascular health and disease.

  9. Adenosine transport systems on dissociated brain cells from mouse, guinea-pig, and rat

    Energy Technology Data Exchange (ETDEWEB)

    Johnston, M.E.; Geiger, J.D. (Univ. of Manitoba, Winnipeg (Canada))

    1990-09-01

    The kinetics and sodium dependence of adenosine transport were determined using an inhibitor-stop method on dissociated cell body preparations obtained from mouse, guinea-pig and rat brain. Transport affinity (KT) values for the high affinity adenosine transport systems KT(H) were significantly different between these three species; mean +/- SEM values were 0.34 +/- 0.1 in mouse, 0.9 +/- 0.2 in rat, and 1.5 +/- 0.5 microM in guinea-pig. The KT values for the low affinity transport system KT(L) were not different between the three species. Brain cells from rat displayed a significantly greater maximal capacity to accumulate (3H)adenosine (Vmax) than did mouse or guinea-pig for the high affinity system, or than did mouse for the low affinity system. When sodium chloride was replaced in the transport medium with choline chloride, the KT(H) values for guinea-pig and rat were both increased by approximately 100%; only in rat did the change reach statistical significance. The sodium-dependence of adenosine transport in mouse brain was clearly absent. The differences between KT(H) values in mouse and those in guinea-pig or rat were accentuated in the absence of sodium. The differences in kinetic values, ionic requirements, and pharmacological characteristics between adenosine transporters in CNS tissues of mouse, guinea-pig and rat may help account for some of the variability noted among species in terms of their physiological responses to adenosine.

  10. Hygienic aspects of livestock manure management and biogas systems operated by small-scale pig farmers in Vietnam.

    Science.gov (United States)

    Huong, Luu Quynh; Madsen, Henry; Anh, Le Xuan; Ngoc, Pham Thi; Dalsgaard, Anders

    2014-02-01

    Biogas digesters are widely promoted and increasingly used to treat and generate gas from pig slurry worldwide. The objective of this study was to describe manure management practices with focus on biogas digestion among small scale pig farmers in Hue (50 farmers) and Hanoi (96 farmers) and to assess fecal contamination levels in biogas effluent. Results showed that 84% of the farmers in Hanoi and 42% in Hue used both pig slurry and human excreta for biogas production. Biogas digestion only reduced E. coli concentrations by 1 to 2 log units to 3.70 ± 0.84 Escherichia coli (log10) cfu/ml on average in effluent as compared with raw slurry. Biogas effluent was commonly used to fertilize vegetables or discharged directly into the garden or aquatic recipients. Reduced problems with bad smells and flies were reported as main reasons for establishing a biogas digester. Further studies are needed to assess human and animal health hazards associated with the discharge and use of biogas effluent from small-scale biogas systems.

  11. Intratracheal dosing with disodium cromoglycate inhibits late asthmatic response by attenuating eicosanoid production in guinea pigs.

    Science.gov (United States)

    Nabe, Takeshi; Yamamoto, Maki; Suga, Mikiko; Kohno, Shigekatsu

    2004-08-16

    Disodium cromoglycate is an anti-asthmatic drug that has mast cell-stabilizing effects and other anti-inflammatory effects. However, the mechanisms of its anti-inflammatory effects are unclear. In this study, we evaluated effects of disodium cromoglycate on eosinophilia, early and late asthmatic responses, and production of arachidonic acid metabolites in guinea pig lungs. Guinea pigs were alternately sensitized and challenged by exposure to mists of ovalbumin+Al(OH)(3) and ovalbumin, respectively. Disodium cromoglycate (0.5-2 mg/0.1 ml/animal) administered intratracheally before the fifth challenge dose-dependently inhibited asthmatic response, but early asthmatic response was not affected. Disodium cromoglycate at 2 mg/animal potently suppressed increases in cysteinyl leukotrienes (CysLTs) and thromboxane A(2) in the lung during late asthmatic response. Eosinophilia was slightly reduced by disodium cromoglycate. The inhibitory effect of disodium cromoglycate on late asthmatic response is apparently due to inhibition of the release of arachidonic acid metabolites, some of which may be derived from eosinophils that infiltrate the lung.

  12. An Electronic Worker Service System of the Pig House Based on ATmega16

    Science.gov (United States)

    Li, Liu-An; Jin, Tian-Ming; Yu, Ya-Ping; Zhang, Guo-Qiang; Hong, Tao

    Pork is very important for people in daily life. Quality of livestock environment is closely related to the growth of animals. To improve environmental quality, the paper designed an electronic worker service system of the pig house based on ATmega16, which consisted of 6 detection modules, wireless data collecting, GSM, time circuit, display, anti-th alarm and music player, etc. A small LAN was constructed by wireless transceiver module to test and collect data of temperature, humidity and harmful gas concentration in the pig house. If one detection point exists that harmful pollutant gases concentration exceeds the standard value, GSM module took a message for livestock farmers, so that farmers can promptly arrive at the pig house to ventilate or deal with manure in the pig house, which can effectively prevent from bad things. Time of pyroelectric detector deployment can be freely set, and intrusion information was timely sent to the users. The anti-th alarm function was achieved by the auxiliary light. The music playing module is open by timer module in order to soothing pigs' life state. All are suitable for small farms and can save human resources.

  13. Potential development of compressed bio-methane gas production from pig farms and elephant grass silage for transportation in Thailand.

    Science.gov (United States)

    Dussadee, Natthawud; Reansuwan, Kamoldara; Ramaraj, Rameshprabu

    2014-03-01

    This research project evaluated biogas production using anaerobic co-digestion of pig manure and elephant grass silage in large scale to delivered transportation directly for cars. Anaerobic co-digestion was estimated in three full-scale continuously stirred tank reactors (CSTRs) at 40°C. In the form of compressed bio-methane gas (CBG) production was 14,400m(3)/day (CH4 60-70%) amount of CBG was 9600m(3)/day. The procedure was enhanced by using molecular sieve, activated carbon for removal of moisture and CO2 membrane H2S and CO2 respectively. The results were demonstrated the amount of CO2, H2S gas was reduced along with CH4 was improved up to 90% by volume and compressed to 250bar tank pressure gauge to the fuel for cars. The CBG production, methane gas improvement and performance were evaluated before entering the delivered systems according to the energy standards. The production of CBG is advantageous to strengthen the Thailand biogas market.

  14. The use of acute phase proteins for monitoring animal health and welfare in the pig production chain: the validation of an immunochromatographic method for the detection of elevated levels of pig-MAP.

    Science.gov (United States)

    Piñeiro, Matilde; Morales, Joaquín; Vizcaíno, Elena; Murillo, José Alberto; Klauke, Thorsten; Petersen, Brigitte; Piñeiro, Carlos

    2013-11-01

    The serum concentration of acute phase proteins (APPs) increases in the presence of disease or stress, which makes APPs notable parameters for the global assessment of animal health and welfare. A rapid, immunochromatographic test (ICT) for the detection of elevated levels of pig Major Acute-phase Protein (pig-MAP), one of the main APPs in pigs, was evaluated in more than 1400 pig serum samples obtained from commercial farms. The ICT showed a good performance with a relative sensitivity (Sn) and specificity (Sp) of 94 and 97%, respectively, for a threshold of 1.5mg/mL (comparison with ELISA). Differences in the pig-MAP levels and the number of positive samples with the ICT were observed within the season of sampling, farms, and age groups at one farm, according to the presence of disease or lesions. The ICT was also evaluated in blood samples obtained at slaughter in association with the carcase inspection. The results from this study indicate that the ICT may be used for the evaluation of groups of pigs, after analysing one sub-sample of these pigs, and might be a useful tool in routine health and welfare monitoring programmes aimed to improve the quality of pig production.

  15. Guinea pig-adapted foot-and-mouth disease virus with altered receptor recognition can productively infect a natural host.

    Science.gov (United States)

    Núñez, José I; Molina, Nicolas; Baranowski, Eric; Domingo, Esteban; Clark, Stuart; Burman, Alison; Berryman, Stephen; Jackson, Terry; Sobrino, Francisco

    2007-08-01

    We report that adaptation to infect the guinea pig did not modify the capacity of foot-and-mouth disease virus (FMDV) to kill suckling mice and to cause an acute and transmissible disease in the pig, an important natural host for this pathogen. Adaptive amino acid replacements (I(248)-->T in 2C, Q(44)-->R in 3A, and L(147)-->P in VP1), selected upon serial passages of a type C FMDV isolated from swine (biological clone C-S8c1) in the guinea pig, were maintained after virus multiplication in swine and suckling mice. However, the adaptive replacement L(147)-->P, next to the integrin-binding RGD motif at the GH loop in VP1, abolished growth of the virus in different established cell lines and modified its antigenicity. In contrast, primary bovine thyroid cell cultures could be productively infected by viruses with replacement L(147)-->P, and this infection was inhibited by antibodies to alphavbeta6 and by an FMDV-derived RGD-containing peptide, suggesting that integrin alphavbeta6 may be used as a receptor for these mutants in the animal (porcine, guinea pig, and suckling mice) host. Substitution T(248)-->N in 2C was not detectable in C-S8c1 but was present in a low proportion of the guinea pig-adapted virus. This substitution became rapidly dominant in the viral population after the reintroduction of the guinea pig-adapted virus into pigs. These observations illustrate how the appearance of minority variant viruses in an unnatural host can result in the dominance of these viruses on reinfection of the original host species.

  16. Energy production systems engineering

    CERN Document Server

    Blair, Thomas Howard

    2017-01-01

    Energy Production Systems Engineering presents IEEE, Electrical Apparatus Service Association (EASA), and International Electrotechnical Commission (IEC) standards of engineering systems and equipment in utility electric generation stations. Electrical engineers that practice in the energy industry must understand the specific characteristics of electrical and mechanical equipment commonly applied to energy production and conversion processes, including the mechanical and chemical processes involved, in order to design, operate and maintain electrical systems that support and enable these processes. To aid this understanding, Energy Production Systems Engineeringdescribes the equipment and systems found in various types of utility electric generation stations. This information is accompanied by examples and practice problems. It also addresses common issues of electrical safety that arise in electric generation stations.

  17. Defining product service systems

    DEFF Research Database (Denmark)

    McAloone, Timothy Charles; Andreasen, Mogens Myrup

    2002-01-01

    artefact and instead provides what the customer really wants the actual functionality from the product. This enables a series of potential improvements to the product´s performance throughout its lifecycle. The ideal of product service system (PSS) development is that all three stakeholder groups customer...... attempt to identify the nature of such a multiple definition of PSS, the link to proper understanding of value and utility and innovative approaches for PSS-oriented product development. This paper will expand on the phenomenon of PSS in the belief that a proper understanding of PSS will give us...

  18. Defining product service systems

    DEFF Research Database (Denmark)

    McAloone, Timothy Charles; Andreasen, Mogens Myrup

    2002-01-01

    , for example, a factor 20 improvement in our environmental performance. One attempt, however, has recently emerged, which combines the product as an artefact with the service that the product provides to the user. Through the combination of these two facets, the company retains ownership of the physical...... artefact and instead provides what the customer really wants the actual functionality from the product. This enables a series of potential improvements to the product´s performance throughout its lifecycle. The ideal of product service system (PSS) development is that all three stakeholder groups customer......, company and society benefit from the service systems related to each one of these dimensions, rather than simply one of the above. There are existing examples of the enhancement of business and market share by focusing on PSS, but this is often not a result of upfront strategy and ambitious goals. We...

  19. Predictive ability of the feeding system in Iberian pig by means of several analytical methods

    Directory of Open Access Journals (Sweden)

    González, E.

    2013-04-01

    Full Text Available The reliability of several analytical methods proposed to predict the feeding system received by Iberian pigs during the fattening period has been contrasted. Samples of subcutaneous adipose tissue were analyzed from 38 batches of pigs fattened in three seasons of montanera (acorn and pastures feeding. They corresponded to the four categories described in the Quality Standard for Iberian pig products: 251 samples of bellota, 164 of recebo, 197 of cebo de campo and 137 of cebo. To perform the study, the following analytical methods were used: fatty acid quantification, NIR, alpha and gamma tocopherol, chemical sensor, triacilglycerides, stable isotopes and neophytadiene. The laboratories received the samples without any information about the fattening system to which they belonged and remitted their predictions with respect to the above categories. The results showed a high percentage of accurate predictions of the methods when the fattening type corresponds to the extreme categories (bellota and cebo, however, the methods had difficulty in discerning between samples from a mixed feeding regime with acorn and feed (recebo and problems when pigs are fattened with compound feeds including vegetable fats with high levels of oleic acid. Although a simplification into only two categories (cebo/campo and recebo/bellota results in a success rate higher than 90% for some methods, the combination of two or three techniques with problematic samples allows for differentiating among the four categories with the same accuracy.Se ha contrastado la fiabilidad de varios métodos analíticos para determinar el tipo de alimentación recibida durante el cebo por cerdos Ibéricos a partir de muestras de tejido adiposo subcutáneo, procedentes de 38 partidas de cerdos de tres campañas de montanera. Las muestras correspondieron con las categorías de alimentación contempladas en la Norma de Calidad: 251 fueron de bellota, 164 de recebo, 197 de campo y 137 de cebo

  20. Effects of two different dietary fermentable carbohydrates on activity and heat production in group-housed growing pigs

    NARCIS (Netherlands)

    Rijnen, M.M.J.A.; Verstegen, M.W.A.; Heetkamp, M.J.W.; Schrama, J.W.

    2003-01-01

    The effects of two sources of dietary fiber (DF) on behavior and heat production (HP) in group-housed growing pigs were studied. Twenty clusters of 14 barrows (50 kg) were fed one of 10 diets. Diets differed mainly in type,and content of fermentable DF (fDF) and in content of digestible starch. Five

  1. Investigation of the effect of UV-LED exposure conditions on the production of vitamin D in pig skin

    DEFF Research Database (Denmark)

    Barnkob, Line Lundbæk; Argyraki, Aikaterini; Petersen, Paul Michael

    2016-01-01

    study the exposure conditions with ultraviolet (UV) LEDs were systematically investigated in the wavelength range 280-340 nm for achieving optimal vitamin D bio-fortification in pig skin. A wavelength of 296 nm was found to be optimal for vitamin D3 production. The maximum dose of 20 kJ/m2 produced 3...

  2. Sociable swine : indirect genetic effects on growth rate and their effect on behaviour and production of pigs in different environments

    NARCIS (Netherlands)

    Camerlink, I.

    2014-01-01

    Social interactions between pigs can influence their health, welfare, and productivity. The effects of social interactions on individuals are partly genetic, and this genetic effect is known as an Indirect Genetic Effect. IGEs are thus the heritable effects of an individual on the trait values of it

  3. Integrating animal manure-based bioenergy production with invasive species control: A case study at Tongren Pig Farm in China

    Energy Technology Data Exchange (ETDEWEB)

    Lu, Jianbo; Zhu, Lei [Institute of Agro-Ecology and Ecological Engineering, College of Life Sciences, Zijingang Campus, Zhejiang University, Hangzhou 310058 (China); Hu, Guoliang [Rural Energy Section, Agricultural Bureau of Haining City, Zhejiang Province 314400 (China); Wu, Jianguo [Institute of Agro-Ecology and Ecological Engineering, College of Life Sciences, Zijingang Campus, Zhejiang University, Hangzhou 310058 (China); School of Life Sciences and Global Institute of Sustainability, Arizona State University, Tempe, AZ 85287-4501 (United States)

    2010-06-15

    Integrated approach and bioresource engineering are often required to deal with multiple and interactive environmental problems for sustainable development at local and regional scales. Pig farming has flourished with fast growing economy and increasing human demands for meat in China. Water hyacinth (Eichhornia crassipes), a noxious invasive species, has encroached into most of the local rivers and lakes. Both the wastes from the booming pig farms as well as the massive plant materials of water hyacinth have caused a range of serious ecological and environmental problems. Here we present an integrated sustainable, ecological and experimental study that was designed to deal with these two problems simultaneously. Our experimental results showed that the mixtures of water hyacinth with pig manure consistently had much higher biogas production than pig manure alone, and that the highest biogas production was achieved when 15% of the fermentation substrates were water hyacinth. Our analysis further revealed that the changing C/N ratio and the lignin content in the fermentation feedstock due to the addition of water hyacinth might be two important factors affecting the biogas production. We also found that the solar-powered water-heating unit significantly increased the biogas production (especially in winter time). Overall, the project proved to be successful ecologically and socially. Through such an integrated approach and bioresource engineering, wastes are treated, energy is harvested, and the environment is protected. (author)

  4. Effects of two different dietary fermentable carbohydrates on activity and heat production in group-housed growing pigs

    NARCIS (Netherlands)

    Rijnen, M.M.J.A.; Verstegen, M.W.A.; Heetkamp, M.J.W.; Schrama, J.W.

    2003-01-01

    The effects of two sources of dietary fiber (DF) on behavior and heat production (HP) in group-housed growing pigs were studied. Twenty clusters of 14 barrows (50 kg) were fed one of 10 diets. Diets differed mainly in type,and content of fermentable DF (fDF) and in content of digestible starch. Five

  5. Productivity Enhancement through Production Monitoring System

    OpenAIRE

    Prasad, Shiva H C; Rao, Potti Srinivasa; Gopalkrishna, B; Ahluwalia, Aakash

    2017-01-01

    A production monitoring system uses the real-time data while production is online. The real-time production monitoring systems are designed as means of auto data to the collection and monitoring the data via display boards. This study focuses on analysing the real-time production monitoring systems through trend analysis in production and over consumption of raw material controlling the over consumptions in a pen manufacturing industry. The methodology followed is through process flow diagram...

  6. Establishment and evaluation of the system of extracorporeal liver perfusion in pigs.

    Science.gov (United States)

    Gong, Jin; Wang, Xi-Mo; Long, Gang; Guo, Zhong-Tao; Jiang, Tao; Chen, Shi

    2005-02-01

    In recent years, extracorporeal liver perfusion (ECLP) has been regarded as a treatment of acute liver failure (ALF); but the system of ECLP has many problems. The purpose of this experiment was to detect the factors affecting the system of ECLP and to establish a stable and effective system of ECLP. Livers were harvested from health pigs, according to the different styles of perfusion and oxygenation, which were randomly divided into 3 groups. The livers in group A (n=4) were subjected to single portal vein perfusion, oxygenating perfusion blood; the livers in group B (n=4) to dual (portal vein and hepatic artery) vessel perfusion, oxygenating blood, together; and the livers in group C (n=4) to dual (portal vein and hepatic artery) vessel perfusion, but oxygenating blood, separately. The perfusion time, the data of bile production, and hemodynamic parameters of extracorporeal livers in each group were tested. The histological examination of liver tissues from each group was performed at the end of perfusion. The perfusion time of the liver in group A is significantly shorter than in groups B and C (P0.05). But at 12 hours after perfusion, the data of group B were statistically different from those of livers in group C (Pportal vein and hepatic artery) vessel perfusion and oxygenating blood separately, is more stable and effective to keep the function of extracorporeal liver.

  7. In vitro digestion and fermentation characteristics of canola co-products simulate their digestion in the pig intestine.

    Science.gov (United States)

    Woyengo, T A; Jha, R; Beltranena, E; Zijlstra, R T

    2016-06-01

    Canola co-products are sources of amino acid and energy in pig feeds, but their fermentation characteristics in the pig intestine are unknown. Thus, we determined the in vitro fermentation characteristics of the canola co-products Brassica juncea solvent-extracted canola meal (JSECM), Brassica napus solvent-extracted canola meal (NSECM), B. napus expeller-pressed canola meal (NEPCM) and B. napus cold-pressed canola cake (NCPCC) in comparison with soybean meal (SBM). Samples were hydrolysed in two steps using pepsin and pancreatin. Subsequently, residues were incubated in a buffer solution with fresh pig faeces as inocula for 72 h to measure gas production. Concentration of volatile fatty acids (VFA) per gram of dry matter (DM) of feedstuff was measured in fermented solutions. Apparent ileal digestibility (AID) and apparent hindgut fermentation (AHF) of gross energy (GE) for feedstuffs were obtained from pigs fed the same feedstuffs. On DM basis, SBM, JSECM, NSECM, NEPCM and NCPCC contained 15, 19, 22, 117 and 231 g/kg ether extract; and 85, 223, 306, 208 and 176 g/kg NDF, respectively. In vitro digestibility of DM (IVDDM) of SBM (82.3%) was greater (Pfermentation characteristics of canola co-products and SBM simulated their fermentation in the small and large intestine of pigs, respectively. The 30% greater VFA production for JSECM than NSECM due to lower lignified fibre of JSECM indicates that fermentation characteristics differ between canola species. The NSECM had the highest fermentability followed by NEPCM and then NCPCC, indicating that fat in canola co-products can limit their fermentability in the hindgut.

  8. Evaluation of immune system function in neonatal pigs born vaginally or by Cesarean section

    Science.gov (United States)

    Eight full term crossbred sows were selected for study of the interaction of the immune system, hypothalamus-pituitary-adrenal axis, and growth in pigs born by Cesarean section (C-section) or vaginal-birth (n=4 each for vaginal-birth and C-section). Gestation length and birth weight did not differ b...

  9. Morphological analysis of the vestibular system of guinea pigs poisoned by organophosphate

    Directory of Open Access Journals (Sweden)

    Lícia Assunção Cogo

    2016-02-01

    Full Text Available ABSTRACT INTRODUCTION: The vestibular system is responsible for body balance. There are substances that damage it, causing dizziness; these are termed vestibulotoxic substances. Agrochemicals have been investigated for ototoxicity because of studies that identified dizziness as a recurrent symptom among rural workers' complaints. OBJECTIVE: To histopathologically evaluate the vestibular system in guinea pigs exposed to an organophosphate, and to identify the drug's effects on this system. METHODS: Experimental clinical study. Eighteen guinea pigs were used; six of them poisoned with the organophosphate chlorpyrifos at doses of 0.5 mg/kg/day and seven of them at 1 mg/kg/day; and a control group of five guinea pigs was exposed to distilled water, all for 10 consecutive days. Later, ciliary tufts of saccule and utricle maculae were counted by scanning electron microscopy. RESULTS: Comparing the groups, a one-way ANOVA test for the variable "saccule" ( p = 0.0569 and a Kruskal-Wallis test for the variable "utricle" ( p = 0.8958 were performed, revealing no difference among groups in both variables. CONCLUSION: The histopathologic analysis of the vestibular system of guinea pigs exposed to an organophosphate showed no difference in the amount of ciliary tufts of saccule and utricle maculae at the doses tested, although the result for the variable "saccule" was considered borderline, showing a trend for significance.

  10. Evaluation of the sustainability of contrasted pig farming systems: integrated evaluation

    NARCIS (Netherlands)

    Bonneau, M.; Klauke, T.N.; Gonzalez, J.; Rydhmer, L.; Ilari-Antoine, E.; Dourmad, J.Y.; Greef, de K.H.; Houwers, H.W.J.; Cinar, M.U.; Fabrega, E.; Zimmer, C.; Hviid, M.; Oever, van der B.; Edwards, S.A.

    2014-01-01

    The aim of this paper is to present an approach for an integrated evaluation of the sustainability of pig farming systems, taking into account the three classical pillars: economy, environment and society. Eight sustainability themes were considered: Animal Welfare (AW), Animal Health (AH), Breeding

  11. Strengths, weaknesses, opportunities and threats of the pig health monitoring systems used in England.

    Science.gov (United States)

    Stärk, K D C; Nevel, A

    2009-10-17

    Several systems are being used in England to record information about the health of pigs. The British Pig Health Scheme (BPHS), the National Animal Disease Information System (NADIS), the Zoonoses Action Plan (ZAP) for Salmonella and the Veterinary Investigation Diagnosis Analysis (VIDA) system have been assessed to make recommendations for their future separate or joint development. The structure, organisation, processes, data quality, dissemination, utilisation and acceptance of each system have been assessed. Information was extracted from documents and websites, and informal interviews were conducted with technical experts and stakeholders. The systems covered a broad range of objectives, used variable approaches and operated at very different scales and budgets. There was a high level of awareness and involvement by the industry. Common weaknesses of the systems were the lack of in-depth quantitative analysis of the data, the lack of assessment of each system's impact, and the unknown level of bias as a result of the voluntary or selective participation in them.

  12. Indications and patterns of therapeutic use of antimicrobial agents in the Danish pig production from 2002 to 2008

    DEFF Research Database (Denmark)

    Jensen, Vibeke Frøkjær; Emborg, Hanne-Dorthe; Aarestrup, Frank Møller

    2011-01-01

    This study describes trends in the use and indications for prescriptions of antimicrobial agents in the Danish pig production in the period between 2002 and 2008 and is the first description of a complete prescription pattern for one animal species in an entire country. Data on all prescription....../piglets, by 141% for weaning pigs, and by 81% for finisher pig. The most commonly used class of antibiotics was tetracycline for all age-groups, replacing the previously used macrolide/lincosamide group. The use of pleuromutilin increased in 2008 to the level of macrolides. In sow/piglets, the second most used...... class was penicillins. The switch in choice of antimicrobial classes prescribed seems to be related primarily to changes in the price of the drugs....

  13. Producción Fúngica de Proteasas Inducidas con Pelo de Cerdo Fungal Production of Proteases Induced with Pig Hair

    Directory of Open Access Journals (Sweden)

    Imir R Vázquez

    2008-01-01

    Full Text Available Se evaluaron las condiciones de producción de proteasas fúngicas inducidas con pelo de cerdo usando dos sistemas de cultivo, el proceso de cultivo en estado sólido y el cultivo líquido sumergido. El medio de cultivo fue Czapek-Dox en ambos casos. La cinética fue monitoreada cada 24 horas durante un periodo de incubación de 120 horas. La actividad proteolítica fue evaluada por hidrólisis de azocaseína y la producción de la biomasa fue estimada por el método gravimétrico. Rhizopus oryzae fue el microorganismo capaz de utilizar el pelo de cerdo como única fuente de carbono-nitrógeno, produciendo en cultivo sumergido los mayores títulos de actividad queratinasa. Se determinó el efecto que tiene el pH, el pretratamiento del sustrato y la adición de diferentes niveles de material queratinoso sobre el crecimiento fúngico y la actividad enzimática. Los resultados permitieron obtener títulos de actividad proteolítica de 975 U/L.The production of new proteases induced with pig hair was evaluated using two systems, submerged culture and solid state culture. Culture medium was Czapek-Dox for both systems. Enzyme production kinetic was monitored each 24 hours during 120 hours. Protelytic activity was evaluated with the azocaseín method and biomass production was determined by the gravimetric method. Rhizopus oryzae was the microorganism with the capacity of using the pig hair as the single carbon-nitrogen source, producing in submerged fermentation the highest enzymatic titers. The effect of pH, the substrate pretreatment and addition of different levels of substrate on proteolyitic activity and biomass production were determined. Results permitted to obtain titers of proteolytic activity of 975 U/L.

  14. Dynamics of Air Temperature, Velocity and Ammonia Emissions in Enclosed and Conventional Pig Housing Systems

    Directory of Open Access Journals (Sweden)

    J. I. Song

    2013-03-01

    Full Text Available This study aimed to compare the dynamics of air temperature and velocity under two different ventilation and housing systems during summer and winter in Korea. The NH3 concentration of both housing systems was also investigated in relation to the pig’s growth. The ventilation systems used were; negative pressure type for the enclosed pig house (EPH and natural airflow for the conventional pig house (CPH. Against a highly fluctuating outdoor temperature, the EPH was able to maintain a stable temperature at 24.8 to 29.1°C during summer and 17.9 to 23.1°C during winter whilst the CPH had a wider temperature variance during summer at 24.7 to 32.3°C. However, the temperature fluctuation of the CPH during winter was almost the same with that of EPH at 14.5 to 18.2°C. The NH3 levels in the CPH ranged from 9.31 to 16.9 mg/L during summer and 5.1 to 19.7 mg/L during winter whilst that of the EPH pig house was 7.9 to 16.1 mg/L and 3.7 to 9.6 mg/L during summer and winter, respectively. These values were less than the critical ammonia level for pigs with the EPH maintaining a lower level than the CPH in both winter and summer. The air velocity at pig nose level in the EPH during summer was 0.23 m/s, enough to provide comfort because of the unique design of the inlet feature. However, no air movement was observed in almost all the lower portions of the CPH during winter because of the absence of an inlet feature. There was a significant improvement in weight gain and feed intake of pigs reared in the EPH compared to the CPH (p<0.05. These findings proved that despite the difference in the housing systems, a stable indoor temperature was necessary to minimize the impact of an avoidable and highly fluctuating outdoor temperature. The EPH consistently maintained an effective indoor airspeed irrespective of season; however the CPH had defective and stagnant air at pig nose level during winter. Characteristics of airflow direction and pattern were

  15. haematological profiles of pigs raised under intensive management ...

    African Journals Online (AJOL)

    EZE J I

    MANAGEMENT SYSTEM IN SOUTH-EASTERN NIGERIA. *EZE, J. I. ... SUMMARY. The haematological profile of pigs raised under intensive management system in South- ... Monogastric (both swine and poultry), has made a ... pig production has played a significant role in meat ... adversely affect the health or welfare of.

  16. Effects of two different dietary fermentable carbohydrates on activity and heat production in group-housed growing pigs.

    Science.gov (United States)

    Rijnen, M M J A; Verstegen, M W A; Heetkamp, M J W; Schrama, J W

    2003-05-01

    The effects of two sources of dietary fiber (DF) on behavior and heat production (HP) in group-housed growing pigs were studied. Twenty clusters of 14 barrows (50 kg) were fed one of 10 diets. Diets differed mainly in type and content of fermentable DF (fDF) and in content of digestible starch. Five diets contained solvent-extracted coconut meal (SECM) and five diets contained soybean hulls (SBH) as the main fDF source. On an as-fed basis, pigs received 3.5, 13.2, 23.0, 32.7, or 42.4 g x kg(-0.75) x d(-1) of SECM or SBH. A total of 280 crossbred growing pigs were used, divided into clusters of 14 pigs each. Pigs were group-housed and fed at 2.5 times the assumed maintenance energy requirements. All clusters were fed similar amounts of NE, ileal-digestible protein and amino acids, vitamins, and minerals. Consequently, DMI differed among diets because NE content decreased with increasing DF content. After a 32-d preliminary period, HP was measured per cluster during a 7-d experimental period in environmentally controlled respiration chambers. Behavior of the pigs was recorded using time-lapse video recordings during two different days within the experimental period. Intake of digestible starch and fDF was different (P average, pigs spent 153 min standing, 42 min sitting, 202 min lying on their chest, and 1,043 min lying on their flanks each day. Pigs fed SECM diets spent, on average, less time (P time spent on physical activity (i.e., standing plus sitting, 195 min/d) was not affected by diet. Total HP and resting HP were affected by diet and were on average lower (P averaged 65 kJ x kg(-0.75) x d(-1) and was not affected by diet. There was a linear relationship (P < 0.001) between fDF intake and HP, but there was no relationship between fDF intake and AHP. During different parts of the day, fDF intake also affected HP. The saving effect of physical activity on the NE values of fDF from SECM and SBH were 0.56 and 0.84 kJ/g of fDF intake, respectively. Neither of

  17. ATLAS production system

    CERN Document Server

    Borodin, Mikhail; The ATLAS collaboration; De, Kaushik; Klimentov, Alexei; Golubkov, Dmitry; Maeno, Tadashi; Mashinistov, Ruslan; Wenaus, Torre; Padolski, Siarhei

    2016-01-01

    The second generation of the ATLAS production system called ProdSys2 is a distributed workload manager which used by thousands of physicists to analyze the data remotely, with the volume of processed data is beyond the exabyte scale, across a more than hundred heterogeneous sites. It achieves high utilization by combining dynamic job definition based on many criterias, such as input and output size, memory requirements and CPU consumption with manageable scheduling policies and by supporting different kind of computational resources, such as GRID, clouds, supercomputers and volunteering computers. Besides jobs definition Production System also includes flexible web user interface, which implements user-friendly environment for main ATLAS workflows, e.g. simple way of combining different data flows, and real-time monitoring, optimised for using with huge amount of information to present. We present an overview of the ATLAS Production System major components: job and task definition, workflow manager web user i...

  18. Determining the effects of early gestation in utero heat stress on postnatal fasting heat production and circulating biomarkers associated with metabolism in growing pigs

    Science.gov (United States)

    The study objective was to determine the effects of in utero heat stress (IUHS) on postnatal fasting heat production (FHP) in growing pigs. Based on our previous observation of increased postnatal core body temperature ‘set-point’ in IUHS pigs, we hypothesized that FHP would be greater during postna...

  19. Modulation of the acute phase response following a lipopolysaccharide challenge in pigs supplemented with an all-natural Saccharomyces cerevisiae fermentation product

    Science.gov (United States)

    This study was designed to determine if feeding a Saccharomyces cerevisiae fermentation product to weaned pigs would reduce the stress and acute phase responses (APR) following an acute lipopolysaccharide (LPS) challenge. Pigs (n = 20; 6.4 +/- 0.2 kg body weight) were obtained and transported to an ...

  20. Human mast cell mediator cocktail excites neurons in human and guinea-pig enteric nervous system.

    Science.gov (United States)

    Schemann, M; Michel, K; Ceregrzyn, M; Zeller, F; Seidl, S; Bischoff, S C

    2005-04-01

    Neuroimmune interactions are an integral part of gut physiology and involved in the pathogenesis of inflammatory and functional bowel disorders. Mast cells and their mediators are important conveyors in the communication from the innate enteric immune system to the enteric nervous system (ENS). However, it is not known whether a mediator cocktail released from activated human mast cells affects neural activity in the ENS. We used the Multi-Site Optical Recording Technique to image single cell activity in guinea-pig and human ENS after application of a mast cell mediator cocktail (MCMC) that was released from isolated human intestinal mucosa mast cells stimulated by IgE-receptor cross-linking. Local application of MCMC onto individual ganglia evoked an excitatory response consisting of action potential discharge. This excitatory response occurred in 31%, 38% or 11% neurons of guinea-pig submucous plexus, human submucous plexus, or guinea-pig myenteric plexus, respectively. Compound action potentials from nerve fibres or fast excitatory synaptic inputs were not affected by MCMC. This study demonstrates immunoneural signalling in the human gut and revealed for the first time that an MCMC released from stimulated human intestinal mast cells induces excitatory actions in the human and guinea-pig ENS.

  1. Development of Production Systems

    DEFF Research Database (Denmark)

    Christiansen, Thomas Bøhm

    1997-01-01

    This paper presents the initial considerations related to a Ph.D. study initiated at IPV, DTU in February 1997, concerning the research subject "Development of Production Systems". The content and aim of this paper is to 1) to introduce the study by......This paper presents the initial considerations related to a Ph.D. study initiated at IPV, DTU in February 1997, concerning the research subject "Development of Production Systems". The content and aim of this paper is to 1) to introduce the study by...

  2. Development of Production Systems

    DEFF Research Database (Denmark)

    Christiansen, Thomas Bøhm

    1997-01-01

    This paper presents the initial considerations related to a Ph.D. study initiated at IPV, DTU in February 1997, concerning the research subject "Development of Production Systems". The content and aim of this paper is to 1) to introduce the study by......This paper presents the initial considerations related to a Ph.D. study initiated at IPV, DTU in February 1997, concerning the research subject "Development of Production Systems". The content and aim of this paper is to 1) to introduce the study by...

  3. Foraging behaviour, nutrient intake from pasture and performance of free-range growing pigs in relation to feed CP level in two organic cropping systems

    DEFF Research Database (Denmark)

    Jakobsen, Malene; Kongsted, Anne Grete; Hermansen, John Erik

    2015-01-01

    In organic pig production one of the major challenges is to be able to fulfil amino acid requirements based on organic and locally grown protein feed crops. The pig is an opportunistic omnivore with a unique capacity for foraging above and below the soil surface. It is hypothesized that direct...... foraging in the range area can pose an important contribution in terms of fulfilling nutrient requirements of growing pigs. Foraging activity, lucerne nutrient intake and pig performance were investigated in 36 growing pigs, foraging on lucerne or grass and fed either a standard organic pelleted feed...... of pasture/pig per day during the 40 days experimental period from September to October 2013. Behavioural observations were carried out 12 times over the entire experimental period. For both crops, LP pigs rooted significantly more compared with HP pigs but the effect of CP level was more pronounced in grass...

  4. Comparison of Performance, Meat Lipids and Oxidative Status of Pigs from Commercial Breed and Organic Crossbreed

    Directory of Open Access Journals (Sweden)

    Giuseppe Martino

    2014-06-01

    Full Text Available The aim of this research was to determine the effect of rearing systems for pig production, as concerns performance, meat lipid content, the fatty acid profile, histidinic antioxidants, coenzyme Q10, and TBARs. One hundred pigs were assigned to one of three treatments: intensively reared commercial hybrid pig (I, free range commercial hybrid pig (FR or organically reared crossbred pig (O, according to organic EU Regulations. I pigs showed the best productive performance, but FR and O increased: C20:1n9, Δ9-desaturase (C18 and thioesterase indices in meat. Lipid, dipeptides and CoQ10 appeared correlated to glycolytic and oxidative metabolic pathways. We can conclude that all studied parameters were influenced by the rearing system used, and that differences were particularly evident in the O system, which produced leaner meat with higher oxidative stability. In this respect, the organic pig rearing system promotes and enhances biodiversity, environmental sustainability and food quality.

  5. Public Attitudes to Housing Systems for Pregnant Pigs.

    Directory of Open Access Journals (Sweden)

    E B Ryan

    Full Text Available Understanding concerns about the welfare of farm animals is important for the development of socially sustainable production practices. This study used an online survey to test how views on group versus stall housing for pregnant sows varied when Canadian and US participants were provided information about these systems, including access to scientific papers, YouTube videos, Google images, and a frequently-asked-questions page (S1 Appendix. Initial responses and changes in responses after accessing the information were analyzed from Likert scores of 242 participants and from their written comments. Participants were less willing to accept the use of gestation stalls after viewing information on sow housing. For example, initially 30.4% of respondents indicated that they supported the use of gestation stalls; this declined to 17.8% after participants were provided additional information. Qualitative analysis of comments showed that supporters of gestation stalls expressed concern about the spread of disease and aggression between animals in less confined systems, whereas supporters of group housing placed more emphasis on the sow's ability to interact socially and perform natural behaviors. These results point to public opposition to the use of gestation stalls, and indicate that the more that the public learns about gestation stalls the less willing they will be to accept their use.

  6. Assessment of the potential productivity of pigs in the Teso and ...

    African Journals Online (AJOL)

    Mo

    For peri-urban areas, where there is no much land, pig farming was a good ... Litter size. 7 piglets. 7-12 piglets. Number of farrowings per year. 2 times. 1-2 times .... four to rank nine parameters across cattle, goats, pigs and chickens in the two ...

  7. Evaluation of the sustainability of contrasted pig farming systems: integrated evaluation.

    Science.gov (United States)

    Bonneau, M; Klauke, T N; Gonzàlez, J; Rydhmer, L; Ilari-Antoine, E; Dourmad, J Y; de Greef, K; Houwers, H W J; Cinar, M U; Fàbrega, E; Zimmer, C; Hviid, M; van der Oever, B; Edwards, S A

    2014-12-01

    The aim of this paper is to present an approach for an integrated evaluation of the sustainability of pig farming systems, taking into account the three classical pillars: economy, environment and society. Eight sustainability themes were considered: Animal Welfare (AW), Animal Health (AH), Breeding Programmes (BP), Environment (EN), Meat Safety (MS), Market Conformity (MC), Economy (EC) and Working Conditions (WC). A total of 37 primary indicators were identified and used for the evaluation of 15 much contrasted pig farming systems in five EU countries. The results show that the eight themes were not redundant and all contributed to the observed variation between systems. The tool was very robust for highlighting the strengths and weaknesses of the systems along the eight themes that were considered. The number of primary indicators could be reduced from 37 to 18 with limited impact on the strengths/weaknesses profile of the individual systems. Integrating the eight theme evaluations into a single sustainability score is based on hypotheses or presumptions on the relative weights that should be given to the eight themes, which are very dependent on the context and on the purpose of the users of the tool. Therefore, the present paper does not have the ambition to provide a ready-for-use tool, rather to suggest an approach for the integrated evaluation of the sustainability of pig farming systems.

  8. Ultrasonic characterisation of B. femoris from Iberian pigs of different genetics and feeding systems.

    Science.gov (United States)

    Niñoles, L; Mulet, A; Ventanas, S; Benedito, J

    2011-10-01

    Ultrasonic velocity was used to characterise the differences in composition and texture of Biceps Femoris muscles from four batches of pigs of different genetics (Iberian and Iberian × Duroc) and feeding systems ("montanera" and concentrate). Significant differences (p<0.05) were found for the ultrasonic velocity in samples with different genetics and feeding systems. These differences were dependent on the temperature of the measurements and were related to the intramuscular fat content (IMF) of the samples and, therefore, to the meat quality. The ultrasonic velocities at 0 and 20 °C were related to the IMF (R=0.77 and 0.65, respectively). A discriminant analysis, including ultrasonic velocity at temperatures from 0 to 20 °C, allowed 87.0% of the samples to be correctly classified in the batches. Therefore, ultrasonics could be useful in the characterisation and differentiation of B. femoris muscles of Iberian pigs with different genetics and from different feeding systems.

  9. Effects of the Truck Suspension System on Animal Welfare, Carcass and Meat Quality Traits in Pigs

    Science.gov (United States)

    Dalla Costa, Filipe Antônio; Lopes, Letícia S.; Dalla Costa, Osmar Antônio

    2017-01-01

    Simple Summary Transportation is a complex stressor in which animals are exposed to a series negatively stimuli, such as vibration, new environmental conditions, variation in temperature and humidity, social mixing, noises among other poor factors, which can result in welfare problems and economic losses such as increased skin lesions, poorer pork quality traits. Transport stress may be reduced through a vehicle suspension system that provides a much smoother ride during transport, and consequently is less aversive to pigs. However, air suspension systems are more expensive and have bigger maintenance costs. This increase in transportation cost must be supported by the benefits from improvements in quality of freight transport; otherwise, the truckers will be paying unnecessarily for a similar or equivalent ride quality. Thus, finishing pigs were assessed after transport to slaughter by the same two double-decked trucks using two types of commercial vehicle suspension, leaf-spring and air suspension, to compare effects on blood cortisol and lactate at exsanguination, behaviour during lairage, and carcass (skin lesions) and pork quality traits. The use of leaf-spring suspension system negatively affects the welfare of pigs due to the increased carcass damage and resulted in poorer pork quality traits. Abstract The objective of this study was to assess the effects of two types of commercial suspension (leaf-spring (LS) vs. air suspension (AS)) installed on two similar double-decked trucks on blood cortisol and lactate concentration, lairage behavior, carcass skin lesions and pork quality traits of 120 crossbred pigs. The suspension type neither influenced pig behaviour in lairage nor blood cortisol and lactate concentrations (p > 0.10). However, when compared with the AS suspension system, the use of LS increased the number of skin lesions in the back and thigh (p = 0.03 and p = 0.01, respectively) and produced thigh with lower pHu (p < 0.001) and yellower colour

  10. Environmental impact of heavy pig production in a sample of Italian farms. A cradle to farm-gate analysis.

    Science.gov (United States)

    Pirlo, G; Carè, S; Casa, G Della; Marchetti, R; Ponzoni, G; Faeti, V; Fantin, V; Masoni, P; Buttol, P; Zerbinatti, L; Falconi, F

    2016-09-15

    Four breeding piggeries and eight growing-fattening piggeries were analyzed to estimate potential environmental impacts of heavy pig production (>160kg of live height at slaughtering). Life Cycle Assessment methodology was adopted in the study, considering a system from breeding phase to growing fattening phase. Environmental impacts of breeding phase and growing-fattening phase were accounted separately and then combined to obtain the impacts of heavy pig production. The functional unit was 1kg of live weight gain. Impact categories investigated were global warming (GW), acidification (AC), eutrophication (EU), abiotic depletion (AD), and photochemical ozone formation (PO). The total environmental impact of 1kg of live weight gain was 3.3kg CO2eq, 4.9E-2kg SO2eq, 3.1E-2kg PO4(3-)eq, 3.7E-3kg Sbeq, 1.7E-3kg C2H4eq for GW, AC, EU, AD, and PO respectively. Feed production was the main hotspot in all impact categories. Greenhouse gases responsible for GW were mainly CH4, N2O, and CO2. Ammonia was the most important source of AC, sharing about 90%. Nitrate and NH3 were the main emissions responsible for EU, whereas P and NOx showed minor contributions. Crude oil and natural gas consumption was the main source of AD. A large spectrum of pollutants had a significant impact on PO: they comprised CH4 from manure fermentation, CO2 caused by fossil fuel combustion in agricultural operations and industrial processes, ethane and propene emitted during oil extraction and refining, and hexane used in soybean oil extraction. The farm characteristics that best explained the results were fundamentally connected with performance indicators Farms showed a wide variability of results, meaning that there was wide margin for improving the environmental performance of either breeding or growing-fattening farms. The effectiveness of some mitigation measures was evaluated and the results that could be obtained by their introduction have been presented.

  11. Quantification of hepatitis E virus in naturally-contaminated pig liver products

    Directory of Open Access Journals (Sweden)

    Sandra Martin-Latil

    2016-08-01

    Full Text Available Hepatitis E virus (HEV, the cause of self-limiting acute hepatitis in humans, is widespread and endemic in many parts of the world. The foodborne transmission of HEV has become of concern due to the identification of undercooked pork products as a risk factor for infection. Foodborne enteric viruses are conventionally processed by quantitative RT-PCR (RT-qPCR, which gives sensitive and quantitative detection results. Recently, digital PCR (dPCR has been described as a novel approach to genome quantification with no need for a standard curve. The performance of microfluidic digital RT-PCR (RT-dPCR was compared to RT-qPCR when detecting HEV in pig liver products. The sensitivity of the RT-dPCR assay was similar to that of RT-qPCR, and quantitative data obtained by both detection methods were not significantly different for almost all samples. This absolute quantification approach may be useful for standardising quantification of HEV in food samples and may be extended to quantifying other human pathogens in food samples.

  12. Production of Cloned Korean Native Pig by Somatic Cell Nuclear Transfer.

    Science.gov (United States)

    Hwang, In-Sul; Kwon, Dae-Jin; Oh, Keun Bong; Ock, Sun-A; Chung, Hak-Jae; Cho, In-Cheol; Lee, Jeong-Woong; Im, Gi-Sun; Hwang, Seongsoo

    2015-06-01

    The Korean native pig (KNP) have been considered as animal models for animal biotechnology research because of their relatively small body size and their presumably highly inbred status due to the closed breeding program. However, little is reported about the use of KNP for animal biotechnology researches. This study was performed to establish the somatic cell nuclear transfer (SCNT) protocol for the production of swine leukocyte antigens (SLA) homotype-defined SCNT KNP. The ear fibroblast cells originated from KNP were cultured and used as donor cell. After thawing, the donor cells were cultured for 1 hour with 15 μM roscovitine prior to the nuclear transfer. The numbers of reconstructed and parthenogenetic embryos transferred were 98 ± 35.2 and 145 ± 11.2, respectively. The pregnancy and delivery rate were 3/5 (60%) and 2/5 (40%). One healthy SLA homotype-defined SCNT KNP was successfully generated. The recipient-based individual cloning efficiency ranged from 0.65 to 1.08%. Taken together, it can be postulated that the methodological establishment of the production of SLA homotype-defined cloned KNP can be applied to the generation of transgenic cloned KNP as model animals for human disease and xenotransplantation researches.

  13. ESTIMATION OF GENETIC PARAMETERS FOR PRODUCTION TRAITS IN PIG BREEDS IN CROATIA

    Directory of Open Access Journals (Sweden)

    Anamarija Smetko

    2015-09-01

    Full Text Available Genetic parameters for backfat thickness and test time were updated and used in genetic evaluation for field tested pigs in Croatia. Data consisted of 9,406 animals with measurements for production traits recorded from 2000 to 2014. The number of animals in pedigree was 10,728. Production traits were modelled using a single trait animal model including the following fixed class effects: breed, sex, classifier, season, and herd. Weight at the end of the test was included as linear regression in the model for backfat. Direct additive genetic effect, interaction herd-year-season of testing and common litter effect were included as random effects. Variance components were estimated using REML method as implemented in the VCE-6 program. The estimated heritabilities were 0.28±0.03 for backfat thickness and 0.12±0.02 for test time. Litter effect accounted from 15 to 24% of phenotypic variation, while herd-year-season of testing explained additional 24 and 28% of variability for analysed traits.

  14. PORK CARCASS COMPOSITION AND THE MEAT QUALITY OF THE BLACK SLAVONIAN PIG – THE ENDANGERED BREEDS IN THE INDOOR AND OUTDOOR KEEPING SYSTEM

    Directory of Open Access Journals (Sweden)

    Danijela Butko

    2007-06-01

    Full Text Available The research has been made on 20 Black Slavonian Pigs in both ways of keeping them (indoor, outdoor. The pigs have been fed up to 135 kg body weight. The pigs in the outdoor system have been held on a natural pasture ground. Other than pasture, the pigs have consumed, over the summer period, the food offered on stubble-fields after the harvest (barley, wheat and over the winter times after corn harvest. They had only minimal corn consumption; mostly during the winter (average daily consumption was 0.15 kg. Dissection of cold (+40C right sided pig body composition has been made by the modified Weniger et al (1963 method. The meat quality has been determined on a long back muscle sample (musculus longissimus dorsi- MLD taken berween 13th and 14th rib. The pig carcasses in the outdoor system had a very significant (P<0.01 absolute and relative leg share, less belly-rib share and higher meat quantity. Commercially, more valuable parts-legs and back had a greater share of muscle tissue in pigs' carcasses in the outdoor system. The meat of the pigs in the outdoor system had no signifficant differences from the pigs in the indoor system, concerning the pH1, pH2, water holding capacity, colour and marbling. However, the meat of the pigs in the outdoor system had higher content of crude fat from the pigs in the indoor system.

  15. Partitioning of heat production in growing pigs as a tool to improve the determination of efficiency of energy utilization

    Directory of Open Access Journals (Sweden)

    Etienne eLabussière

    2013-06-01

    Full Text Available In growing pigs, the feed cost accounts for more than 60% of total production costs. The determination of efficiency of energy utilization through calorimetry measurements is of importance to sustain suitable feeding practice. The objective of this paper is to describe a methodology to correct daily heat production (HP obtained from measurements in respiration chamber for the difference in energy expenditure related to physical activity between animals. The calculation is based on a preliminary published approach for partitioning HP between HP due to physical activity (AHP, thermic effect of feeding (TEF and basal metabolic rate (FHP. Measurements with male growing pigs (mean body weight: 115 kg which were surgically castrated (SC, castrated through immunization against GnRH (IC, or kept as entire male (EM were used as an example. Animals were fed the same diet ad libitum and were housed individually in two 12-m3 open-circuit respiration chambers during six days when fed ad libitum and one supplementary day when fasted. Physical activity was recorded through interruption of an infrared beam to detect standing and lying positions and with force transducers that recorded the mechanical force the animal exerted on the floor of the cage. Corrected AHP (AHPc, TEF (TEFc and HP (HPc were calculated to standardize the level of AHP between animals, assuming that the ratio between AHPc and ME intake should be constant. Inefficiency of energy utilization (sum of AHPc and TEFc was lower than the inefficiency estimated from the slope of the classical relationship between HPc and ME intake but was associated with higher requirements for maintenance. Results indicate that EM pigs had higher FHP but lower TEFc than IC and SC pigs. These results agree with the higher contents in viscera of EM pigs that stimulate their basal metabolic rate and to the reduced utilization of dietary protein to provide energy for maintenance energy requirements and fat deposition.

  16. Analyses of intestinal commensal Escherichia coli strains from wild boars suggest adaptation to conventional pig production conditions.

    Science.gov (United States)

    Römer, Antje; Wieler, Lothar H; Schierack, Peter

    2012-12-28

    To test the hypothesis that Escherichia coli populations have adapted to conventional pig production practices, we comparatively tested intestinal commensal E. coli from wild boars versus isolates from domestic pigs by analyzing virulence-associated factors, adhesion, and metabolic activities. Virulence-associated genes typical for intestinal pathogenic E. coli (inVAGs) were sporadically detected among E. coli from wild boars except the adhesion-related gene paa and the enterotoxin-encoding gene astA. In contrast, several VAGs typical for extraintestinal pathogenic E. coli (exVAGs) were common in E. coli from wild boars. The exVAG chuA occurred more often in E. coli from wild boars compared to E. coli from domestic pigs. 23.5% of E. coli from wild boars belonged to EcoR group B2 which is higher than observed for E. coli from clinically healthy domestic pigs. Furthermore, E. coli from wild boars were more efficient in fermentation of carbohydrate sources (dulcitol, inositol, d-sucrose, d-tagatose), and adhered better to the intestinal porcine epithelial cell line IPEC-J2. In conclusion, our findings point towards an adaptation of porcine intestinal E. coli to a specific intestinal milieu caused by different animal living conditions.

  17. Technological Options to Ameliorate Waste Treatment of Intensive Pig Production in China:An Analysis Based on Bio-Economic Model

    Institute of Scientific and Technical Information of China (English)

    LU Wen-cong; MA Yong-xi; Holger Bergmann

    2014-01-01

    Ameliorating waste treatment by technological improvements affects the economic and the ecological-environment beneifts of intensive pig production. The objective of the research was to develop and test a method to determine the technical optimization to ameliorate waste treatment methods and gain insight into the relationship between technological options and the economic and ecological effects. We developed an integrated bio-economic model which incorporates the farming production and waste disposal systems to simulate the impact of technological improvements in pig manure treatment on economic and environmental benefits for the case of a pilot farm in Beijing, China. Based on different waste treatment technology options, three scenarios are applied for the simulation analysis of the model. The simulation results reveal that the economic-environmental beneifts of the livestock farm could be improved by reducing the cropland manure application and increasing the composting production with the current technologies. Nevertheless, the technical efifciency, the waste treatment capacity and the economic beneifts could be further improved by the introduction of new technologies. It implies that technological and economic support policies should be implemented comprehensively on waste disposal and resource utilization to promote sustainable development in intensive livestock production in China.

  18. Production of transgenic pigs over-expressing the antiviral gene Mx1

    OpenAIRE

    2014-01-01

    The myxovirus resistance gene (Mx1) has a broad spectrum of antiviral activities. It is therefore an interesting candidate gene to improve disease resistance in farm animals. In this study, we report the use of somatic cell nuclear transfer (SCNT) to produce transgenic pigs over-expressing the Mx1 gene. These transgenic pigs express approximately 15–25 times more Mx1 mRNA than non-transgenic pigs, and the protein level of Mx1 was also markedly enhanced. We challenged fibroblast cells isolated...

  19. STRATEGIES FOR INCREASING PRODUCTIVITY IN PRODUCTION SYSTEMS

    Directory of Open Access Journals (Sweden)

    Diego Pacheco

    2014-05-01

    Full Text Available The main objective of this article is to point a set of practical strategies that can be adopted to increase the capacity of constraints resources on production systems, when the constraint is inside the factory and not is in the market. To serve this purpose will be presented strategies based on best practices of the Theory of Constraints, Lean Manufacturing and Total Productive Maintenance. This article also presents the mains tools for the deployment of these methodologies. The survey results have provided an objective set of practical strategy that can be used to increase the capacity and productivity of production systems according to the needs of each manufacturing system.

  20. Production monitoring system for understanding product robustness

    DEFF Research Database (Denmark)

    Boorla, Srinivasa Murthy; Howard, Thomas J.

    2016-01-01

    In the current quality paradigm, the performance of a product is kept within specification by ensuring that its parts are within specification. Product performance is then validated after final assembly. However, this does not control how robust the product performance is, i.e. how much...... it will vary between the specification limits. In this paper, a model for predicting product performance is proposed, taking into account design, assembly and process parameters live from production. This empowers production to maintain final product performance, instead of part quality. The PRECI‐IN case...... study is used to demonstrate how the monitoring system can be used to efficiently guide corrective action to improve product performance. It is claimed that the monitoring system can be used to dramatically cut the time taken to identify, planand execute corrective action related to typical quality...

  1. Escherichia coli contamination of fish raised in integrated pig-fish aquaculture systems in Vietnam.

    Science.gov (United States)

    Dang, Son Thi Thanh; Dalsgaard, Anders

    2012-07-01

    Integrated livestock-fish aquaculture utilizes animal excreta and urine as pond fertilizers to enhance growth of plankton and other microorganisms eaten by the fish. In Vietnam, pigs are commonly integrated with fish and horticulture in household-based VAC systems (vuon = garden; ao = pond; chuong = pigsty), but the level of fecal contamination in the fish produced is unknown. This study was carried out to assess the level Escherichia coli contamination of fish meat and gut contents of grass carp (Ctenopharyngodon idellus), silver carp (Hypophthalmichthys molitrix), and rohu (Labeo rohita) cultured in randomly selected five VAC ponds (with pig manure) and five non-VAC ponds (without pig manure) at sites in periurban Hanoi, Vietnam. Fish muscle tissue samples contained E. coli at <10 or 320 or 820 CFU/g, regardless of the culture system from which they originated. In contrast, the intestinal contents of fish raised in manure-fed ponds contained E. coli at 4.75, 5.25, and 5.07 log CFU/g for silver carp, grass carp, and rohu, respectively, about 100 times higher than the contamination of fish from the control ponds. The results indicate that muscle tissue of fish raised in VAC systems has a low level of fecal contamination despite high levels of E. coli in their gut. Thus, a critical point to control food safety of such fish is the prevention of fecal cross-contamination during degutting and cleaning of the fish at markets and in the home.

  2. Production of methane by co-digestion of cassava pulp with various concentrations of pig manure

    Energy Technology Data Exchange (ETDEWEB)

    Panichnumsin, Pornpan [The Joint Graduate School of Energy and Environment, King Mongkut' s University of Technology Thonburi, Thungkru, Bangkok 10140 (Thailand); Excellent Center of Waste Utilization and Management, National Center for Genetic Engineering and Biotechnology, Bangkhuntien, Bangkok 10150 (Thailand); Nopharatana, Annop [Pilot Plant Development and Training Institute, King Mongkut' s University of Technology Thonburi, Bangkhuntien, Bangkok 10150 (Thailand); Ahring, Birgitte [AAU, Copenhagen Institute of Technology, Lautrupvang 15, 2750 Ballerup (Denmark); Chaiprasert, Pawinee [School of Bioresources and Technology, King Mongkut' s University of Technology Thonburi, Bangkhuntien, Bangkok 10150 (Thailand)

    2010-08-15

    Cassava pulp is a major by-product produced in a cassava starch factory, containing 50-60% of starch (dry basis). Therefore, in this study we are considering its potential as a raw material substrate for the production of methane. To ensure sufficient amounts of nutrients for the anaerobic digestion process, the potential of co-digestion of cassava pulp (CP) with pig manure (PM) was further examined. The effect of the co-substrate mixture ratio was carried out in a semi-continuously fed stirred tank reactor (CSTR) operated under mesophilic condition (37 C) and at a constant OLR of 3.5 kg VS m{sup -3} d{sup -1} and a HRT of 15 days. The results showed that co-digestion resulted in higher methane production and reduction of volatile solids (VS) but lower buffering capacity. Compared to the digestion of PM alone, the specific methane yield increased 41% higher when co-digested with CP in concentrations up to 60% of the incoming VS. This was probably due to an increase in available easily degradable carbohydrates as the CP ratio in feedstock increased. The highest methane yield and VS removal of 306 mL g{sup -1} VS{sub added} and 61%, respectively, were achieved with good process stability (VFA:Alkalinity ratio < 0.1) when CP accounted for 60% of the feedstock VS. A further increase of CP of the feedstock led to a decrease in methane yield and solid reductions. This appeared to be caused by an extremely high C:N ratio of the feedstock resulting in a deficiency of ammonium nitrogen for microbial growth and buffering capacity. (author)

  3. Produção pecuária no montado: suínos Livestock production in the “montado”: pigs

    Directory of Open Access Journals (Sweden)

    J. T. Nunes

    2007-01-01

    feedstuffs resources (pastures, acorns etc. and local breeds. In Portugal, during the first half of last century Alentejano pig breed represented almost 50% of total pigs raised in the country. Intensification on agricultural systems and on livestock management contributed to a strong decrease on extensive system pig herds and in 1986 the amount of autochthonous pigs in Portugal represented about 2%. Local breeds of pigs, sheep, goats or even cattle recently got a new opportunity. Considering high grade products they can apply for an add value, by valorisation of the originality linked to the traditional production system, under oaks or chestnuts canopy. PAC evolution and a progressive environmental concern also helped to restart, on a sustainable way, the finishing animal grazing in open spaces, pigs fattened under acorns (montanheira or chestnuts. That means the fattening of hogs aged 14 to 18 months grazing under oaks, during autumn and winter. This late fattening, performed on a diet rich in starch, produces heavy carcasses, from 120 kg to 140 kg, with high percentage of fat and an important intermuscular infiltration of fat. To recommend a proper livestock management to optimise the carcass and meat characteristics, in farming systems using natural resources it’s necessary to know, objectively, the natural feedstuffs resources, the particular physiology of local breeds, physical and chemical characteristics and sensorial request of traditional products, as well as fresh meat parameters.

  4. Effects of the Truck Suspension System on Animal Welfare, Carcass and Meat Quality Traits in Pigs

    Directory of Open Access Journals (Sweden)

    Filipe Antônio Dalla Costa

    2017-01-01

    Full Text Available The objective of this study was to assess the effects of two types of commercial suspension (leaf-spring (LS vs. air suspension (AS installed on two similar double-decked trucks on blood cortisol and lactate concentration, lairage behavior, carcass skin lesions and pork quality traits of 120 crossbred pigs. The suspension type neither influenced pig behaviour in lairage nor blood cortisol and lactate concentrations (p > 0.10. However, when compared with the AS suspension system, the use of LS increased the number of skin lesions in the back and thigh (p = 0.03 and p = 0.01, respectively and produced thigh with lower pHu (p < 0.001 and yellower colour (higher b* value; p = 0.03, and paler back muscles (subjective colour; p < 0.05, with a tendency to lower pH (p = 0.06. Therefore, the use air suspension system can improve carcass and meat quality traits of pigs transported to slaughter.

  5. Production of transgenic pigs over-expressing the antiviral gene Mx1.

    Science.gov (United States)

    Yan, Quanmei; Yang, Huaqiang; Yang, Dongshan; Zhao, Bentian; Ouyang, Zhen; Liu, Zhaoming; Fan, Nana; Ouyang, Hongsheng; Gu, Weiwang; Lai, Liangxue

    2014-01-01

    The myxovirus resistance gene (Mx1) has a broad spectrum of antiviral activities. It is therefore an interesting candidate gene to improve disease resistance in farm animals. In this study, we report the use of somatic cell nuclear transfer (SCNT) to produce transgenic pigs over-expressing the Mx1 gene. These transgenic pigs express approximately 15-25 times more Mx1 mRNA than non-transgenic pigs, and the protein level of Mx1 was also markedly enhanced. We challenged fibroblast cells isolated from the ear skin of transgenic and control pigs with influenza A virus and classical swine fever virus (CFSV). Indirect immunofluorescence assay (IFA) revealed a profound decrease of influenza A proliferation in Mx1 transgenic cells. Growth kinetics showed an approximately 10-fold reduction of viral copies in the transgenic cells compared to non-transgenic controls. Additionally, we found that the Mx1 transgenic cells were more resistant to CSFV infection in comparison to non-transgenic cells. These results demonstrate that the Mx1 transgene can protect against viral infection in cells of transgenic pigs and indicate that the Mx1 transgene can be harnessed to develop disease-resistant pigs.

  6. Production of human CD59-transgenic pigs by embryonic germ cell nuclear transfer

    Energy Technology Data Exchange (ETDEWEB)

    Ahn, Kwang Sung; Won, Ji Young [Department of Physiology, Dankook University School of Medicine, Cheonan (Korea, Republic of); Park, Jin-Ki [Animal Biotechnology Division, National Institute of Animal Science, Suwon (Korea, Republic of); Sorrell, Alice M. [Department of Physiology, Dankook University School of Medicine, Cheonan (Korea, Republic of); Heo, Soon Young; Kang, Jee Hyun [Department of Nanobiomedical Science, Dankook University, Cheonan (Korea, Republic of); Woo, Jae-Seok [Animal Biotechnology Division, National Institute of Animal Science, Suwon (Korea, Republic of); Choi, Bong-Hwan [Genomics and Bioinformatics Division, National Institute of Animal Science, Suwon (Korea, Republic of); Chang, Won-Kyong [Animal Biotechnology Division, National Institute of Animal Science, Suwon (Korea, Republic of); Shim, Hosup, E-mail: shim@dku.edu [Department of Nanobiomedical Science, Dankook University, Cheonan (Korea, Republic of); Institute of Tissue Regeneration Engineering, Dankook University, Cheonan (Korea, Republic of)

    2010-10-01

    Research highlights: {yields} Human CD59 (hCD59) gene was introduced into porcine embryonic germ (EG) cells. {yields} hCD59-transgenic EG cells were resistant to hyperacute rejection in cytolytic assay. {yields} hCD59-transgenic pigs were produced by EG cell nuclear transfer. -- Abstract: This study was performed to produce transgenic pigs expressing the human complement regulatory protein CD59 (hCD59) using the nuclear transfer (NT) of embryonic germ (EG) cells, which are undifferentiated stem cells derived from primordial germ cells. Because EG cells can be cultured indefinitely in an undifferentiated state, they may provide an inexhaustible source of nuclear donor cells for NT to produce transgenic pigs. A total of 1980 NT embryos derived from hCD59-transgenic EG cells were transferred to ten recipients, resulting in the birth of fifteen piglets from three pregnancies. Among these offspring, ten were alive without overt health problems. Based on PCR analysis, all fifteen piglets were confirmed as hCD59 transgenic. The expression of the hCD59 transgene in the ten living piglets was verified by RT-PCR. Western analysis showed the expression of the hCD59 protein in four of the ten RT-PCR-positive piglets. These results demonstrate that hCD59-transgenic pigs could effectively be produced by EG cell NT and that such transgenic pigs may be used as organ donors in pig-to-human xenotransplantation.

  7. Pesticide Product Information System (PPIS)

    Data.gov (United States)

    U.S. Environmental Protection Agency — The Pesticide Product Information System contains information concerning all pesticide products registered in the United States. It includes registrant name and...

  8. The effect of production type and antimicrobial usage on the occurrence of tetracycline resistant E. coli in danish slaughter pig farms

    DEFF Research Database (Denmark)

    Struve, Tina; Vigre, Håkan; Wingstrand, Anne;

    was estimated in two separate models using logistic regression, taking into account the correlation of results obtained from the same farm. Among the 411 isolates, 129 was found resistant to Tetracycline (Organic: 10%, Free Range: 27 % Conventional: 39 %). Differences was seen in the consumption pattern among...... of antimicrobial resistance, with the lowest occurrence in Organic production and the highest in Conventional production. When analyzing the effect of production type and usage of antimicrobials on occurrence of resistance in the same model, the usage of antimicrobials was not found to have an effect. This lack...... systems. At nine different slaughterhouses 1500 ceacum samples were collected from slaughter pigs originating from 226 farms. One thousand samples were analyzed and one E. coli isolate per sample was susceptibility tested to Tetracycline. Data on management practice and health status at farm level...

  9. Injurious tail biting in pigs: how can it be controlled in existing systems without tail docking?

    Science.gov (United States)

    D'Eath, R B; Arnott, G; Turner, S P; Jensen, T; Lahrmann, H P; Busch, M E; Niemi, J K; Lawrence, A B; Sandøe, P

    2014-09-01

    Tail biting is a serious animal welfare and economic problem in pig production. Tail docking, which reduces but does not eliminate tail biting, remains widespread. However, in the EU tail docking may not be used routinely, and some 'alternative' forms of pig production and certain countries do not allow tail docking at all. Against this background, using a novel approach focusing on research where tail injuries were quantified, we review the measures that can be used to control tail biting in pigs without tail docking. Using this strict criterion, there was good evidence that manipulable substrates and feeder space affect damaging tail biting. Only epidemiological evidence was available for effects of temperature and season, and the effect of stocking density was unclear. Studies suggest that group size has little effect, and the effects of nutrition, disease and breed require further investigation. The review identifies a number of knowledge gaps and promising avenues for future research into prevention and mitigation. We illustrate the diversity of hypotheses concerning how different proposed risk factors might increase tail biting through their effect on each other or on the proposed underlying processes of tail biting. A quantitative comparison of the efficacy of different methods of provision of manipulable materials, and a review of current practices in countries and assurance schemes where tail docking is banned, both suggest that daily provision of small quantities of destructible, manipulable natural materials can be of considerable benefit. Further comparative research is needed into materials, such as ropes, which are compatible with slatted floors. Also, materials which double as fuel for anaerobic digesters could be utilised. As well as optimising housing and management to reduce risk, it is important to detect and treat tail biting as soon as it occurs. Early warning signs before the first bloody tails appear, such as pigs holding their tails tucked

  10. Lifetime and per year productivity of sows in four pig farms in the tropics of Mexico.

    Science.gov (United States)

    Ek-Mex, Jesús Enrique; Segura-Correa, José Candelario; Alzina-López, Alejandro; Batista-Garcia, Laura

    2015-03-01

    The objectives of this study were to estimate the lifetime and per year productivity of sows and to determine the effect of some factor on those traits in four pig farms in the tropics of Mexico. Data from 7526 sows for lifetime number of piglets born alive per sow (LBA), lifetime kilograms of piglets at farrowing (LKF), number of piglets born alive per year (NPF/Y), and kilograms of piglets at farrowing per year (KPF/Y); and data from 7230 sows for lifetime number of piglets weaned (LPW), lifetime kilograms of piglets weaned (LKW), number of piglets weaned per year (NPW/Y), and kilograms of piglets weaned per year (KPW/Y) per sow were used. The statistical model for all traits included the fixed effects of farm, year of first farrowing, season of first farrowing, litter size at first farrowing, age at first farrowing, removal reason, simple interactions, and the error term. The means for LBA, LKF, NPF/Y, and KPF/Y were 45.1 piglets, 67.1 kg, 22.7 piglets, and 33.7 kg, respectively. The means for LPW, LKW, NPW/Y, and KPW/Y were 43.2 piglets, 251.9 kg, 21.5 piglets, and 125.1 kg, respectively. All factors were significant for all traits, except for age at first farrowing on LPW and LKW. Sows with large litter sizes and those that farrowed the first time, at an early age, had the highest lifetime and per year productivity. Therefore, more care and better management should be provided to those types of sows to improve the farms profit.

  11. Analysis of integrated animal-fish production system under subtropical hill agro ecosystem in India: growth performance of animals, total biomass production and monetary benefit.

    Science.gov (United States)

    Kumaresan, A; Pathak, K A; Bujarbaruah, K M; Vinod, K

    2009-03-01

    The present study assessed the benefits of integration of animals with fish production in optimizing the bio mass production from unit land in subtropical hill agro ecosystem. Hampshire pigs and Khaki Campbell ducks were integrated with composite fish culture. The pig and duck excreta were directly allowed into the pond and no supplementary feed was given to fish during the period of study. The average levels of N, P and K in dried pig and duck manure were 0.9, 0.7 and 0.6 per cent and 1.3, 0.6 and 0.5 per cent, respectively. The average body weight of pig and duck at 11 months age was 90 and 1.74 kg with an average daily weight gain of 333.33 and 6.44 g, respectively. The fish production in pig-fish and duck-fish systems were 2209 and 2964 kg/ha, respectively while the fish productivity in control pond was only 820 kg/ha. The total biomass (animal and fish) production was higher (p<0.05) in commercial feeding system compared to the traditional system, however the input/output ratio was 1:1.2 and 1:1.55 for commercial and traditional systems, respectively. It was inferred that the total biomass production per unit land was high (p<0.05) when animal and fish were integrated together.

  12. Evaluation of the sustainability of contrasted pig farming systems: the procedure, the evaluated systems and the evaluation tools.

    Science.gov (United States)

    Bonneau, M; de Greef, K; Brinkman, D; Cinar, M U; Dourmad, J Y; Edge, H L; Fàbrega, E; Gonzàlez, J; Houwers, H W J; Hviid, M; Ilari-Antoine, E; Klauke, T N; Phatsara, C; Rydhmer, L; van der Oever, B; Zimmer, C; Edwards, S A

    2014-12-01

    Although a few studies consider the sustainability of animal farming systems along the three classical main pillars (economy, environment and society), most studies on pig farming systems address only one of these pillars. The present paper is the introduction to a series of companion papers presenting the results of a study undertaken within the EU-supported project Q-PorkChains, aiming at building a comprehensive tool for the evaluation of pig farming systems, which is robust to accommodate the large variability of systems existing in Europe. The tool is mostly based on questions to farmers and comprises a total of 37 dimensions distributed along eight themes: Animal Welfare, Animal Health, Breeding Programmes, Environmental Sustainability, Meat Safety, Market Conformity, Economy and Working Conditions. The paper describes the procedure that was used for building the tool, using it on 15 contrasted pig farming systems and analysing the results. The evaluated systems are briefly described and a short overview of the dimensions is provided. Detailed descriptions of the theme-wise tools and results, as well as the results of an integrated evaluation, are available in the companion papers.

  13. Establishing a DNA identification system for pigs (Sus scrofa) using a multiplex STR amplification.

    Science.gov (United States)

    Lin, Yu-Chih; Hsieh, Hsing-Mei; Lee, James Chun-I; Hsiao, Chung-Ting; Lin, Der-Yuh; Linacre, Adrian; Tsai, Li-Chin

    2014-03-01

    In this study we establish a novel STR multiplex using 13 tetra-nucleotide STRs and the amelogenin marker for the forensic identification of pigs. The genotypes and allele frequency were generated based on 341 samples from 11 pig breeds in Taiwan. Genetic variation was tested including Na, Ne, Ho, He, F-statistics, PIC, Pm and PE for each STR locus and for each breed. Based upon the 341 samples in this study, the CPm and CPEtrio of the 13 STR loci were 1.31 E-11 and 0.9996 respectively. The CPItrio based on ten family sets ranged from 4.012 E+4 to 4.332 E+6 for paternity test. Validation of the multiplex included: determining the sensitivity of the test, where reproducible full DNA profiles were obtained using an initial template of between 0.25 and 1 ng; a comprehensive range of tissue types generated the same genotype; and the specificity was confirmed as no DNA full profile was generated for any species other than Sus scrofa. Based on the phylogenetic analysis, the European domestic breeds clustered separately from the Asian breeds, as expected, and their hybrids formed unique clades respectively between the clades of Asian and European breeds. Eleven test samples, acting as unknown samples, matched all expected breeds. We demonstrate that this novel 14-plex PCR system is valuable in pig individualization, parentage testing, breed assessment, phylogenetic study and forensic applications.

  14. Effects of different space allowances on growth performance, blood profile and pork quality in a grow-to-finish production system.

    Science.gov (United States)

    Jang, J C; Jin, X H; Hong, J S; Kim, Y Y

    2017-06-26

    This experiment was conducted to evaluate the optimal space allowance on growth performance, blood profile and pork quality of growing-finishing pigs. A total of ninety crossbred pigs ([Yorkshire x Landrace] x Duroc, 30.25 ± 1.13 kg) were allocated into three treatments (0.96: four pigs / pen, 0.96 m2/pig; 0.80: five pigs / pen, 0.80 m2/pig; 0.69: six pigs / pen, 0.69 m2/pig) in a randomized complete block (RCB) design. Pigs were housed in balanced sex and had free access to feed in all phases for 14 weeks (growing phase I, growing phase II, finishing phase I and finishing phase II). There was no statistical difference in growing phase, but a linear decrease was observed on average daily gain (ADG, pfinishing phase. On the other hand, a quadratic effect was observed on gain to feed ratio in early finishing phase (pfinish production system is more than 0.80 m2/pig for maximizing growth performance and production efficiency.

  15. Production of α1,3-galactosyltransferase and cytidine monophosphate-N-acetylneuraminic acid hydroxylase gene double-deficient pigs by CRISPR/Cas9 and handmade cloning

    Science.gov (United States)

    GAO, Hanchao; ZHAO, Chengjiang; XIANG, Xi; LI, Yong; ZHAO, Yanli; LI, Zesong; PAN, Dengke; DAI, Yifan; HARA, Hidetaka; COOPER, David K.C.; CAI, Zhiming; MOU, Lisha

    2016-01-01

    Gene-knockout pigs hold great promise as a solution to the shortage of organs from donor animals for xenotransplantation. Several groups have generated gene-knockout pigs via clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) and somatic cell nuclear transfer (SCNT). Herein, we adopted a simple and micromanipulator-free method, handmade cloning (HMC) instead of SCNT, to generate double gene-knockout pigs. First, we applied the CRISPR/Cas9 system to target α1,3-galactosyltransferase (GGTA1) and cytidine monophosphate-N-acetylneuraminic acid hydroxylase (CMAH) genes simultaneously in porcine fetal fibroblast cells (PFFs), which were derived from wild-type Chinese domestic miniature Wuzhishan pigs. Cell colonies were obtained by screening and were identified by Surveyor assay and sequencing. Next, we chose the GGTA1/CMAH double-knockout (DKO) cells for HMC to produce piglets. As a result, we obtained 11 live bi-allelic GGTA1/CMAH DKO piglets with the identical phenotype. Compared to cells from GGTA1-knockout pigs, human antibody binding and antibody-mediated complement-dependent cytotoxicity were significantly reduced in cells from GGTA1/CMAH DKO pigs, which demonstrated that our pigs would exhibit reduced humoral rejection in xenotransplantation. These data suggested that the combination of CRISPR/Cas9 and HMC technology provided an efficient and new strategy for producing pigs with multiple genetic modifications. PMID:27725344

  16. Typology of Product Configuration Systems

    DEFF Research Database (Denmark)

    Jensen, Klaes Ladeby; Edwards, Kasper; Haug, Anders

    2007-01-01

    Many organisations are moving from mass production to mass customization. Product configuration systems (PCS) are increasingly seen as an interesting option for firms who wish to pursue a strategy with a high degree of product variance while retaining a low cost of specifying the product. To become...... more specific in relation to how product configuration systems can support mass customization, it is necessary to understand how different product configuration systems can be classified, and how these differ. This paper presents a typology of product configuration systems based on the five kinds...

  17. Measuring the drinking behaviour of individual pigs housed in group using radio frequency identification (RFID)

    OpenAIRE

    2016-01-01

    Changes in the drinking behaviour of pigs may indicate health, welfare or productivity problems. Automated monitoring and analysis of drinking behaviour could allow problems to be detected, thus improving farm productivity. A high frequency radio frequency identification (HF RFID) system was designed to register the drinking behaviour of individual pigs. HF RFID antennas were placed around four nipple drinkers and connected to a reader via a multiplexer. A total of 55 growing-finishing pigs w...

  18. Amino acid digestibility and concentration of digestible and metabolizable energy in a threonine biomass product fed to weanling pigs.

    Science.gov (United States)

    Almeida, F N; Sulabo, R C; Stein, H H

    2014-10-01

    Production of crystalline l-Thr results in the generation of a Thr biomass that contains more than 80% CP, 5.20% Lys, 5.10% Val, 4.52% Thr, 4.15% Ile, and 1.06% Trp. This Thr biomass product can possibly be used as a feed ingredient in diets fed to weanling pigs, but there is little information about the nutritional value of this product. The objective of this work was to determine the AA digestibility and energy concentration in Thr biomass and to compare these values to values obtained for fish meal in diets fed to pigs. The apparent ileal digestibility (AID) and the standardized ileal digestibility (SID) of CP and AA were determined in Exp. 1. Nine pigs (initial BW: 13.4 ± 2.5 kg) were equipped with a T-cannula in the distal ileum and allotted to a triplicated 3 × 3 Latin square design with 3 diets and 3 periods in each square. One diet contained 20.0% Thr biomass as the sole source of AA, and a second diet contained 25.0% fish meal as the sole source of AA. The last diet was a N-free diet that was used to measure basal endogenous losses of AA and CP. Results indicated that the AID and SID of all AA except Trp, Gly, and Pro were greater (P biomass than in fish meal. In Exp. 2, 24 pigs (initial BW: 18.1 ± 3.5 kg) were placed in metabolism cages and randomly allotted to 3 diets. The first diet contained 96.4% corn, the second diet contained 79.3% corn and 17.0% Thr biomass, and the third diet contained 75.3% corn and 24.0% fish meal. Total collection of feces and urine was performed for 5 d after a 5-d adaptation period, and all samples of ingredients, diets, feces, and urine were analyzed for GE. Digestible energy and ME were then calculated. The DE in the Thr biomass was greater (P biomass was also greater (P biomass is an excellent source of AA and ME. Therefore, the Thr biomass is a viable ingredient that can be used as an alternative to fish meal and possibly other animal proteins in diets for pigs.

  19. Determinants of Biogas Adoption in Manure Management of Vietnamese Household Pig Production : A Case Study in Tien Lu District, Hung Yen Province

    OpenAIRE

    Ly, Nguyen Thi; Nanseki, Teruaki; Chomei, Yosuke

    2015-01-01

    Although biogas technology is one of the useful ways managing the animal manure to reduce environmental problems in rural area, many Vietnamese households that produce pigs still do not apply biogas digesters. This study aim to investigate the main factors contributing to biogas adoption in household pig production based on primary data collected in Tien Lu district, Hung Yen province in Vietnam and the use of Logit regression. The empirical results highlighted that socioeconomic characterist...

  20. Investigation of the effect of UV-LED exposure conditions on the production of vitamin D in pig skin.

    Science.gov (United States)

    Barnkob, Line Lundbæk; Argyraki, Aikaterini; Petersen, Paul Michael; Jakobsen, Jette

    2016-12-01

    The dietary intake of vitamin D is currently below the recommended intake of 10-20μg vitamin D/day. Foods with increased content of vitamin D or new products with enhanced vitamin D are warranted. Light-emitting diodes (LEDs) are a potential new resource in food production lines. In the present study the exposure conditions with ultraviolet (UV) LEDs were systematically investigated in the wavelength range 280-340nm for achieving optimal vitamin D bio-fortification in pig skin. A wavelength of 296nm was found to be optimal for vitamin D3 production. The maximum dose of 20kJ/m(2) produced 3.5-4μg vitamin D3/cm(2) pig skin. Vitamin D3 produced was independent on the combination of time and intensity of the LED source. The increased UV exposure by UV-LEDs may be readily implemented in existing food production facilities, without major modifications to the process or processing equipment, for bio-fortifying food products containing pork skin.

  1. Production of hGFAP-DsRed transgenic Guangxi Bama mini-pigs via somatic cell nuclear transfer.

    Science.gov (United States)

    Hu, L L; Lu, Y Q; Xu, H Y; Yang, X G; Lu, S S; Lu, K H

    2015-12-08

    The mini-pig is a useful animal model for human biomedical research due to its physiological similarity to humans and the ease of handling. In order to optimize the efficiency of production of transgenic Bama mini-pigs through somatic cell nuclear transfer (SCNT), we examined the effects of contact inhibition, roscovitine treatment, and serum starvation on the cell cycle synchronization and transgenic cloned embryo development in vivo and in vitro after nuclear transfer. The analysis showed that the rates of G0/G1 stage cells in the contact inhibition (92.11%) and roscovitine treatment groups (89.59%) were significantly higher than in serum starvation group (80.82%). A higher rate of apoptosis was seen in the serum starvation group (14.13%) compared to the contact inhibition and roscovitine treatment groups (6.71 and 2.46% respectively, P transferred to surrogate sows; one pregnancy was established and three embryos from the roscovitine treatment group successfully completed gestation. These results indicate that the roscovitine treatment was more effective at synchronizing transgenic kidney cells in Bama mini-pigs and allowed reconstructed embryos to develop to full term.

  2. Modelling the kinetic of biogas production from co-digestion of pig waste and grass clippings

    OpenAIRE

    Matheri, Anthony Njuguna; Belaid, Mohamed; Seodigeng, Tumisang, Dr.; Ngila, Catherine Jane

    2016-01-01

    Abstract: This work investigated the use of laboratory batch anaerobic digester to derive kinetics parameters for anaerobic co-digestion of pig waste and grass clippings. Laboratory experiment data from 10 litres batch anaerobic digester operating at ambient mesophilic temperature of 37 0C and pH of 6.9 was used to derive parameters for modified Gompertz model. The carbon/nitrogen (C/N) ratio of Pig waste was found to be 16.16 and grass clippings to be 20.54. Through co-digestion in ratio of ...

  3. Effect of high dietary fat content on heat production and lipid and protein deposition in growing immunocastrated male pigs.

    Science.gov (United States)

    Batorek-Lukač, N; Dubois, S; Noblet, J; Čandek-Potokar, M; Labussière, E

    2016-12-01

    In immunocastrated (IC) pigs, revaccination (V2) increases lipid deposition (LD) because of increased voluntary feed intake; but little is known on associated effect of diet composition on partitioning of nutrients in IC pigs. Digestibility measurements, N and energy balances in respiration chambers were performed in two subsequent stages in four replicates of two male littermates to determine the changes between 85 (stage 1) and 135 (stage 2) kg live weight due to combined effect of IC, growth and increased feed intake (IC/growth). During stage 1, pigs received a standard low-fat diet (LF diet; 2.5% dry matter (DM) of fat fed at 2.27 MJ metabolizable energy (ME)/kg BW0.60 per day), whereas during stage 2, feed intake was increased to 2.47 MJ ME/kg BW0.60 per day and one littermate was fed LF diet whereas the second received a fat-enriched diet (HF diet; 8.9% DM of fat) to determine the effect of increased dietary fat content on energy utilization in IC pigs. Results from N balance and measurements of gas exchanges were used to calculate respiratory quotient (RQ), heat production (HP), nutrient contribution to fat retention, components of HP, protein deposition (PD) and LD. Nutrients and energy apparent digestibility coefficients, methane losses and N retention (Pdietary fat (Ppigs is a result of increased daily LD caused by higher energy intake and lower basal metabolic rate. In addition, LD is further enhanced by dietary energy enrichment with fat after V2.

  4. A combined deficiency of vitamins E and C causes severe central nervous system damage in guinea pigs.

    Science.gov (United States)

    Burk, Raymond F; Christensen, Joani M; Maguire, Mark J; Austin, Lori M; Whetsell, William O; May, James M; Hill, Kristina E; Ebner, Ford F

    2006-06-01

    A short period of combined deficiency of vitamins E and C causes profound central nervous system (CNS) dysfunction in guinea pigs. For this report, CNS histopathology was studied to define the nature and extent of injury caused by this double deficiency. Weanling guinea pigs were fed a vitamin E-deficient or -replete diet for 14 d. Then vitamin C was withdrawn from the diet of some guinea pigs. Four diet groups were thus formed: replete, vitamin E deficient, vitamin C deficient, and both vitamin E and C deficient. From 5 to 11 d after institution of the doubly deficient diet, 9 of 12 guinea pigs developed paralysis, and 2 more were found dead. The remaining guinea pig in the doubly deficient group and all animals in the other 3 groups survived without clinical impairment until the experiment was terminated at 13-15 d. Brains and spinal cords were serially sectioned and stained for examination. Only the combined deficiency produced damage in the CNS. The damage consisted mainly of nerve cell death, axonal degeneration, vascular injury, and associated glial cell responses. The spinal cord and the ventral pons in the brainstem were most severely affected, often exhibiting asymmetric cystic lesions. Several features of the lesions suggest that the primary damage was to blood vessels. These results indicate that the paralysis and death caused by combined deficiency of vitamins E and C in guinea pigs is caused by severe damage in the brainstem and spinal cord.

  5. Induction of systemic IFITM3 expression does not effectively control foot-and-mouth disease viral infection in transgenic pigs.

    Science.gov (United States)

    Zhang, Huawei; Zheng, Haixue; Qian, Ping; Xu, Jinfang; Yang, Xi; Zhou, Rui; Chen, Huanchun; Li, Xiangmin

    2016-08-15

    Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals, and can cause severe economic loss. Interferon-induced transmembrane (IFITM) proteins constitute a family of viral restriction factors that can inhibit the replication of several types of viruses. Our previous study showed that overexpression of swine IFITM3 (sIFITM3) impeded replication of the FMD virus (FMDV) in BHK-21 cells and mice. In this study, sIFITM3-transgenic (TG) pigs were produced by handmade cloning. Results showed that sIFITM3 was highly overexpressed in many organs of sIFITM3-TG pigs compared to wild-type pigs. After a virulent FMDV strain (O/ES/2001) was intramuscularly inoculated, the sIFITM3-TG pigs showed slightly higher susceptibility to FMDV infection than wild-type pigs. Both groups displayed comparable degrees of clinical symptoms throughout the 14-day observation period. Therefore, the induction of systemic sIFITM3 expression does not protect pigs against FMDV infection. Based on these observations, we propose that a combination of interferons and vaccines be used to control FMDV infections and subsequent FMD outbreaks.

  6. A survey of straw use and tail biting in Swedish pig farms rearing undocked pigs

    OpenAIRE

    Wallgren, Torun; Westin, Rebecka; Gunnarsson, Stefan

    2016-01-01

    Background Tail biting is a common problem in intensive pig farming, affecting both welfare and production. Although routine tail docking is banned within the EU, it remains a common practice to prevent tail biting. Straw as environmental enrichment has been proposed as an alternative to tail docking, but its effectiveness against tail biting and function in manure handling systems have to be considered. The aim of the study was to survey how pigs with intact tails are raised and how tail bit...

  7. Changeability of production management systems

    NARCIS (Netherlands)

    Hoogenraad, M. S.; Wortmann, J. C.; Olhager, J; Persson, F

    2007-01-01

    Modem production management systems consist of transaction processing systems and decision enhancement systems. A clear example of two such components are an ERP systems and APS systems. These systems are often standard software systems, and therefore suitable for many different situations. This pap

  8. Changeability of production management systems

    NARCIS (Netherlands)

    Hoogenraad, M. S.; Wortmann, J. C.; Olhager, J; Persson, F

    2007-01-01

    Modem production management systems consist of transaction processing systems and decision enhancement systems. A clear example of two such components are an ERP systems and APS systems. These systems are often standard software systems, and therefore suitable for many different situations. This

  9. Modeling Novo Nordisk Production Systems

    DEFF Research Database (Denmark)

    Miller, Thomas Dedenroth

    1997-01-01

    This report describes attributes of models and systems, and how models can be used for description of production systems. There are special attention on the 'Theory of Domains'.......This report describes attributes of models and systems, and how models can be used for description of production systems. There are special attention on the 'Theory of Domains'....

  10. Immunomodulatory effects of tulathromycin on apoptosis, efferocytosis, and proinflammatory leukotriene B4 production in leukocytes from Actinobacillus pleuropneumoniae-or zymosan-challenged pigs.

    Science.gov (United States)

    Duquette, Stephanie C; Fischer, Carrie D; Williams, Alison C; Sajedy, Saman; Feener, Troy D; Bhargava, Amol; Reti, Kristen L; Muench, Gregory P; Morck, Douglas W; Allison, Jim; Lucas, Merlyn J; Buret, Andre G

    2015-06-01

    To investigate the anti-inflammatory and immunomodulatory properties of tulathromycin in vitro and in experimental models of Actinobacillus pleuropneumoniae-induced pleuropneumonia and zymosan-induced pulmonary inflammation in pigs. Blood samples from six 8- to 30-week-old healthy male pigs for the in vitro experiment and sixty-five 3-week-old specific pathogen-free pigs. Neutrophils and monocyte-derived macrophages were isolated from blood samples. Isolated cells were exposed to tulathromycin (0.02 to 2.0 mg/mL) for various durations and assessed for markers of apoptosis and efferocytosis. For in vivo experiments, pigs were inoculated intratracheally with A pleuropneumoniae, zymosan, or PBS solution (control group) with or without tulathromycin pretreatment (2.5 mg/kg, IM). Bronchoalveolar lavage fluid was collected 3 and 24 hours after inoculation and analyzed for proinflammatory mediators, leukocyte apoptosis, and efferocytosis. In vitro, tulathromycin induced time- and concentration-dependent apoptosis in neutrophils, which enhanced their subsequent clearance by macrophages. In the lungs of both A pleuropneumoniae- and zymosan-challenged pigs, tulathromycin promoted leukocyte apoptosis and efferocytosis and inhibited proinflammatory leukotriene B4 production, with a concurrent reduction in leukocyte necrosis relative to that of control pigs. Tulathromycin also attenuated the degree of lung damage and lesion progression in A pleuropneumoniae-inoculated pigs. Tulathromycin had immunomodulatory effects in leukocytes in vitro and anti-inflammatory effects in pigs in experimental models of A pleuropneumoniae infection and nonmicrobial-induced pulmonary inflammation. These data suggested that in addition to its antimicrobial properties, tulathromycin may dampen severe proinflammatory responses and drive resolution of inflammation in pigs with microbial pulmonary infections.

  11. Strategies for increasing productivity in production systems

    OpenAIRE

    Diego Pacheco; Isaac Pergher; Carlos Fernando Jung; Carla Scwenberg ten Caten

    2014-01-01

    The main objective of this article is to point a set of practical strategies that can be adopted to increase the capacity of constraints resources on production systems, when the constraint is inside the factory and not is in the market. To serve this purpose will be presented strategies based on best practices of the Theory of Constraints, Lean Manufacturing and Total Productive Maintenance. This article also presents the mains tools for the deployment of these methodologies. The survey resu...

  12. Evaluation of in situ valine production by Bacillus subtilis in young pigs

    DEFF Research Database (Denmark)

    Nørgaard, Jan Værum; Canibe, Nuria; Assadi Soumeh, Elham;

    2016-01-01

    Mutants of Bacillus subtilis can be developed to overproduce Val in vitro. It was hypothesized that addition of Bacillus subtilis mutants to pig diets can be a strategy to supply the animal with Val. The objective was to investigate the effect of Bacillus subtilis mutants on growth performance an...

  13. Awareness concerning optimal pig production management and animal welfare among smallholder farmers in Tanzania

    DEFF Research Database (Denmark)

    Braae, Uffe Christian; Penrith, M.-L.; Ngowi, H. A.

    2016-01-01

    The aim of this study was to assess smallholder farmer awareness in terms of good pig management and to identify serious management issues that should be readily changeable despite resources being limited in a rural setting. Methodology was a combination of questionnaire and observational surveys...

  14. Postweaning multisystematic wasting syndrome in Danish pig herds: productivity, clinical signs and pathology

    DEFF Research Database (Denmark)

    Okholm Nielsen, Elisabeth; Enøe, Claes; Jorsal, Sven Erik Lind;

    2008-01-01

    A case-control study of 74 herds with postweaning multisystemic wasting syndrome (PMWS) and 74 matched control herds was carried out. In the case herds the mortality rates of weaner and finisher pigs were 11·2 and 5·2 per cent respectively, compared with 3·1 and 3·2 per cent in the control herds....

  15. Post-weaning multisystemic wasting syndrome (PMWS) in Danish pig herds: productivity, clinical symptoms, and pathology

    DEFF Research Database (Denmark)

    Nielsen, E. Okholm; Enøe, Claes; Jorsal, Sven Erik Lind;

    2008-01-01

    A case-control study of 74 herds with postweaning multisystemic wasting syndrome (PMWS) and 74 matched control herds was carried out. In the case herds the mortality rates of weaner and finisher pigs were 11·2 and 5·2 per cent respectively, compared with 3·1 and 3·2 per cent in the control herds....

  16. Production of germline ablated male pigs via Crispr/Cas editing of the NANOS2 gene

    Science.gov (United States)

    The availability of alternative models to flies, worms, and mice for studying germ cell biology is important for translating findings to higher order mammals. In this context, investigations in pigs and other livestock species can also serve to find applications for both basic biomedical research ...

  17. A description of local pig feeding systems in village smallholder farms of Western Kenya.

    Science.gov (United States)

    Mutua, Florence Kanini; Dewey, Catherine; Arimi, Samuel; Ogara, Wiliam; Levy, Mike; Schelling, Esther

    2012-08-01

    We used face-to-face interviews to gather data on pig feeding practices in rural Busia District, Kenya. We visited 164 pig farms three times in the course of the study period. The pigs were weighed in kilograms during the visits. Feeds offered to pigs were described during the interviews. The most frequently fed feedstuffs were; ground maize or "ugali" (88%), kitchen leftovers (83%) and dried fish locally called "omena" (78%). Farmers provided pigs with water separately from the feeds. Sweet potatoes, "ugali" and cassava were available and could serve as good sources of energy for pigs in the district. Fruits and vegetables were also available and could potentially act as good sources of vitamins. Sweet potato vines, "omena" fish and slaughter blood were available and could provide pigs with proteins. The average daily gain (ADG) for pigs ≤ 5 months of age, pigs of 5.1-9.9 months of age and pigs of ≥ 10 months old was 94.5 (± 43), 127 (± 49.8) and 99 (± 92) g, respectively (p = 0.000). This study has outlined the different local pig feeds available in Busia district. We recommend two things: first, additional research on nutrient composition for the identified local feeds, and second, developing and validating simple local feed combinations that would achieve balanced local pig rations.

  18. Approximate Analysis of Production Systems

    NARCIS (Netherlands)

    M.B.M. de Koster (René)

    1988-01-01

    textabstractIn this paper complex production systems are studied where a single product is manufactured and where each production unit stores its output in at most one buffer and receives its input from at most one buffer. The production units and the buffers may be connected nearly arbitrarily. The

  19. Approximate Analysis of Production Systems

    NARCIS (Netherlands)

    M.B.M. de Koster (René)

    1988-01-01

    textabstractIn this paper complex production systems are studied where a single product is manufactured and where each production unit stores its output in at most one buffer and receives its input from at most one buffer. The production units and the buffers may be connected nearly arbitrarily. The

  20. Meta-analytical study of productive and nutritional interactions of mycotoxins in growing pigs.

    Science.gov (United States)

    Andretta, I; Kipper, M; Lehnen, C R; Hauschild, L; Vale, M M; Lovatto, P A

    2012-09-01

    A meta-analysis was carried out in order to study the association of mycotoxins with performance and organ weights in growing pigs. A total of 85 articles published between 1968 and 2010 were used, totaling 1012 treatments and 13 196 animals. The meta-analysis followed three sequential analyses: graphical, correlation and variance-covariance. The presence of mycotoxins in diets was seen to reduce the feed intake by 18% and the weight gain in 21% compared with the control group. Deoxynivalenol and aflatoxins were the mycotoxins with the greatest impact on the feed intake and growth of pigs, reducing by 26% and 16% in the feed intake and by 26% and 22% in the weight gain. The mycotoxin concentration in diets and the animal age at challenge were the variables that more improved the coefficient of determination in equations for estimating the effect of mycotoxins on weight gain. The mycotoxin effect on growth proved to be greater in younger animals. In addition, the residual analysis showed that the greater part of the variation in weight gain was explained by the variation in feed intake (87%). The protein and methionine levels in diets could influence the feed intake and the weight gain in challenged animals. The weight gain in challenged pigs showed a positive correlation with the methionine level in diets (0.68). The mycotoxin effect on growth was greater in males compared with the effect on females. The reduction in weight gain was of 15% in the female group and 19% in the male group. Mycotoxin presence in pig diets has interfered in the relative weight of the liver, the kidneys and the heart. Mycotoxins have an influence on performance and organ weight in pigs. However, the magnitude of the effects varies with the type and concentration of mycotoxin, sex and the animal age, as well as nutritional factors.

  1. Pig model for diabetes

    DEFF Research Database (Denmark)

    2016-01-01

    The present invention relates to a transgenic pig comprising a mutated IAPP gene and displaying a phenotype associated with diabetes. The invention also relates to a transgenic blastocyst, embryo, fetus, donor cell and/or cell nucleusderived from said transgenic pig. The invention further relates...... to use of the transgenic pig as a model system for studying therapy, treatment and/or prevention of diabetes....

  2. Development of the central nervous system in guinea pig (Cavia porcellus, Rodentia, Caviidae

    Directory of Open Access Journals (Sweden)

    Fernanda Menezes de Oliveira e Silva

    Full Text Available Abstract: This study describes the development of the central nervous system in guinea pigs from 12th day post conception (dpc until birth. Totally, 41 embryos and fetuses were analyzed macroscopically and by means of light and electron microscopy. The neural tube closure was observed at day 14 and the development of the spinal cord and differentiation of the primitive central nervous system vesicles was on 20th dpc. Histologically, undifferentiated brain tissue was observed as a mass of mesenchymal tissue between 18th and 20th dpc, and at 25th dpc the tissue within the medullary canal had higher density. On day 30 the brain tissue was differentiated on day 30 and the spinal cord filling throughout the spinal canal, period from which it was possible to observe cerebral and cerebellar stratums. At day 45 intumescences were visualized and cerebral hemispheres were divided, with a clear division between white and gray matter in brain and cerebellum. Median sulcus of the dorsal spinal cord and the cauda equina were only evident on day 50. There were no significant structural differences in fetuses of 50 and 60 dpc, and animals at term were all lissencephalic. In conclusion, morphological studies of the nervous system in guinea pig can provide important information for clinical studies in humans, due to its high degree of neurological maturity in relation to its short gestation period, what can provide a good tool for neurological studies.

  3. Modelling the potential of slurry management technologies to reduce the constraints of environmental legislation on pig production.

    Science.gov (United States)

    Hutchings, Nicholas J; ten Hoeve, Marieke; Jensen, Rikke; Bruun, Sander; Søtoft, Lene F

    2013-11-30

    Limits on land applications of slurry nitrogen (N) and phosphorus (P) are used to restrict losses of nutrients caused by livestock production. Here, we used a model to assess technologies that enable a more even geographic distribution of slurry nutrients to land. Technologies included were screw press slurry separation, with or without solid fraction composting, centrifuge separation with or without liquid fraction ammonia (NH3) stripping, and anaerobic digestion. Regulatory constraints were placed first on the application in slurry of N, then P, then N and P both on the producing (donor) and receiving (recipient) farms. Finally, a constraint preventing an increase in donor farm NH3 emissions was imposed. Separation had little effect on N losses per unit mass of slurry, but NH3 stripping led to a reduction. Centrifuge separation allowed a greater increase in pig production than a screw press, especially with P regulation. NH3 stripping was only advantageous with N regulation or when combined with NH3 scrubbing of pig housing ventilation air, when donor farm NH3 emissions were a constraint. There was a production penalty for using composting or anaerobic digestion. The choice of appropriate slurry management option therefore depends on the focus of the regulation. Nuanced and therefore complex regulations are necessary to take advantage of synergies and avoid cross-policy conflicts and incongruencies.

  4. Distribution of adrenergic receptors in the enteric nervous system of the guinea pig, mouse, and rat.

    Science.gov (United States)

    Nasser, Yasmin; Ho, Winnie; Sharkey, Keith A

    2006-04-10

    Adrenergic receptors in the enteric nervous system (ENS) are important in control of the gastrointestinal tract. Here we describe the distribution of adrenergic receptors in the ENS of the ileum and colon of the guinea pig, rat, and mouse by using single- and double-labelling immunohistochemistry. In the myenteric plexus (MP) of the rat and mouse, alpha2a-adrenergic receptors (alpha2a-AR) were widely distributed on neurons and enteric glial cells. alpha2a-AR mainly colocalized with calretinin in the MP, whereas submucosal alpha2a-AR neurons colocalized with vasoactive intestinal polypeptide (VIP), neuropeptide Y, and calretinin in both species. In the guinea pig ileum, we observed widespread alpha2a-AR immunoreactivity on nerve fibers in the MP and on VIP neurons in the submucosal plexus (SMP). We observed extensive beta1-adrenergic receptor (beta1-AR) expression on neurons and nerve fibers in both the MP and the SMP of all species. Similarly, the beta2-adrenergic receptor (beta2-AR) was expressed on neurons and nerve fibers in the SMP of all species, as well as in the MP of the mouse. In the MP, beta1- and beta2-AR immunoreactivity was localized to several neuronal populations, including calretinin and nitrergic neurons. In the SMP of the guinea pig, beta1- and beta2-AR mainly colocalized with VIP, whereas, in the rat and mouse, beta1- and beta2-AR were distributed among the VIP and calretinin populations. Adrenergic receptors were widely localized on specific neuronal populations in all species studied. The role of glial alpha2a-AR is unknown. These results suggest that sympathetic innervation of the ENS is directed toward both enteric neurons and enteric glia.

  5. Hygienic aspects of livestock manure management and biogas systems operated by small-scale pig farmers in Vietnam

    DEFF Research Database (Denmark)

    Luu, Huong Quynh; Madsen, Henry; Anh, Le Xuan

    2014-01-01

    Biogas digesters are widely promoted and increasingly used to treat and generate gas from pig slurry worldwide. The objective of this study was to describe manure management practices with focus on biogas digestion among small scale pig farmers in Hue (50 farmers) and Hanoi (96 farmers......) and to assess fecal contamination levels in biogas effluent. Results showed that 84% of the farmers in Hanoi and 42% in Hue used both pig slurry and human excreta for biogas production. Biogas digestion only reduced E. coli concentrations by 1 to 2 log units to 3.70±0.84 Escherichia coli (log) cfu/ml on average...... in effluent as compared with raw slurry. Biogas effluent was commonly used to fertilize vegetables or discharged directly into the garden or aquatic recipients. Reduced problems with bad smells and flies were reported as main reasons for establishing a biogas digester. Further studies are needed to assess...

  6. Colistin in pig production: Chemistry, Mechanism of antibacterial action, Microbial resistance emergence, and One Health Perspectives

    Directory of Open Access Journals (Sweden)

    Mohamed Rhouma

    2016-11-01

    Full Text Available Colistin (Polymyxin E is one of the few cationic antimicrobial peptides commercialized in both human and veterinary medicine. For several years now, colistin has been considered the last line of defense against infections caused by multidrug-resistant (MDR Gram-negative such as Acinetobacter baumannii, Pseudomonas aeruginosa, and Klebsiella pneumoniae. Colistin has been extensively used orally since the 1960s in food animals and particularly in swine for the control of Enterobacteriaceae infections. However, with the recent discovery of plasmid-mediated colistin resistance encoded by the mcr-1 gene and the higher prevalence of samples harboring this gene in animal isolates compared to other origins, livestock has been singled out as the principal reservoir for colistin resistance amplification and spread. Co-localization of the mcr-1 gene and Extended-Spectrum- β-lactamase (ESBL genes on a unique plasmid has been also identified in many isolates from animal origin. The use of colistin in pigs as a growth promoter and for prophylaxis purposes should be banned, and the implantation of sustainable measures in pig farms for microbial infection prevention should be actively encouraged and financed. The scientific research should be encouraged in swine medicine to generate data helping to reduce the exacerbation of colistin resistance in pigs and in manure. The establishment of guidelines ensuring a judicious therapeutic use of colistin in pigs, in countries where this drug is approved, is of crucial importance. The implementation of a microbiological withdrawal period that could reduce the potential contamination of consumers with colistin resistant bacteria of porcine origin should be encouraged. Moreover, the management of colistin resistance at the human-pig-environment interface requires the urgent use of the One Health approach for effective control and prevention. This approach needs the collaborative effort of multiple disciplines and close

  7. Colistin in Pig Production: Chemistry, Mechanism of Antibacterial Action, Microbial Resistance Emergence, and One Health Perspectives

    Science.gov (United States)

    Rhouma, Mohamed; Beaudry, Francis; Thériault, William; Letellier, Ann

    2016-01-01

    Colistin (Polymyxin E) is one of the few cationic antimicrobial peptides commercialized in both human and veterinary medicine. For several years now, colistin has been considered the last line of defense against infections caused by multidrug-resistant Gram-negative such as Acinetobacter baumannii, Pseudomonas aeruginosa, and Klebsiella pneumoniae. Colistin has been extensively used orally since the 1960s in food animals and particularly in swine for the control of Enterobacteriaceae infections. However, with the recent discovery of plasmid-mediated colistin resistance encoded by the mcr-1 gene and the higher prevalence of samples harboring this gene in animal isolates compared to other origins, livestock has been singled out as the principal reservoir for colistin resistance amplification and spread. Co-localization of the mcr-1 gene and Extended-Spectrum-β-Lactamase genes on a unique plasmid has been also identified in many isolates from animal origin. The use of colistin in pigs as a growth promoter and for prophylaxis purposes should be banned, and the implantation of sustainable measures in pig farms for microbial infection prevention should be actively encouraged and financed. The scientific research should be encouraged in swine medicine to generate data helping to reduce the exacerbation of colistin resistance in pigs and in manure. The establishment of guidelines ensuring a judicious therapeutic use of colistin in pigs, in countries where this drug is approved, is of crucial importance. The implementation of a microbiological withdrawal period that could reduce the potential contamination of consumers with colistin resistant bacteria of porcine origin should be encouraged. Moreover, the management of colistin resistance at the human-pig-environment interface requires the urgent use of the One Health approach for effective control and prevention. This approach needs the collaborative effort of multiple disciplines and close cooperation between physicians

  8. Technical note: Evaluation of the official identification system for pigs for sale in New South Wales.

    Science.gov (United States)

    Hernández-Jover, M; Wu, M; Schembri, N; Holyoake, P K; Toribio, J-A L M L

    2008-02-01

    A study was undertaken at 2 saleyard (1 domestic, DS, and 1 export, ES) and 2 abattoir (1 domestic, DA, and 1 export, EA) locations in New South Wales, Australia, to assess the compliance (presence) and readability of body tattoos used to identify individual pigs presented for sale or slaughter. Each location was visited on 3 trading or slaughter days, and tattoo presence and readability of porkers (25 to 60 kg of BW), baconers (60 to 90 kg of BW), backfatters (>90 kg of BW but not for breeding), and breeders were recorded. A total of 4,655 pigs were inspected, including 158 DS, 1,599 ES, 1,257 DA, and 1,641 EA. Tattoo performance at the saleyards was influenced by producer (P 0.05) at DS. At the ES, tattoo compliance among baconers, backfatters, and breeding stock ranged from 82.4 to 88.3% and was greater (P 0.05). Tattoo compliance differed (P system was similar between locations and ranged from 63 to 74%. Our results suggest that current compliance and readability of tattoos would compromise traceback to the farm of origin in the event of an emergency animal disease outbreak. Education activities on legislation requirements and tattoo procedure would likely increase compliance and performance of the system.

  9. Criação intensiva de suínos em confinamento ou ao ar livre: estudo meta-analítico do desempenho zootécnico nas fases de crescimento e terminação e avaliação de carcaça e carne no Longissimus dorsi Intensive pig production in confinement or outdoor systems: a meta-analytical study of the production performance in the growing and finishing phases and carcass and meat evaluation from the Longissimus dorsi

    Directory of Open Access Journals (Sweden)

    Alice Bogoni Demori

    2012-07-01

    Full Text Available Uma meta-análise foi realizada para avaliar o desempenho e a qualidade de carcaça e carne de suínos em função do sistema de alojamento nas fases de crescimento/terminação. Foram selecionadas publicações indexadas com resultados de experimentos comparando variáveis de desempenho e de pós-abate de suínos alojados em sistemas intensivos convencionais ou ao ar livre. A base de dados contemplou 27 artigos publicados entre 1997 e 2009. Ao total, foram considerados 2.985 animais e 701 médias na análise de dados. A meta-análise foi realizada através de duas análises sequenciais: gráfica e de variância-covariância. O sistema de criação de suínos ao ar livre aumentou em 9% (P0,05 pelo sistema de alojamento dos suínos. Concluiu-se que o sistema de criação influencia no desempenho dos suínos, mas não interfere nas características de carcaça e carne estudadas.A meta-analysis was carried out to study performance and characteristics of carcass and meat in pigs in function of housing system during the growing/finishing period. Indexed publications with results of experiments comparing performance and post-slaughter variables of pigs housed in outdoor or conventional intensive systems were selected. In total, 2.985 animals and 701 averages were considered in the data analysis. Meta-analysis followed two sequential analyses: graphic and variance. The outdoor productive system increased in 9% (P0.05 by the housing system. It is concluded that the housing system influences the pig performance, but does not interfere in characteristics of carcass and meat.

  10. Estimating farm-gate ammonia emissions from major animal production systems in China

    Science.gov (United States)

    Gao, Zhiling; Ma, Wenqi; Zhu, Gaodi; Roelcke, Marco

    2013-11-01

    Ammonia (NH3) emissions from livestock production in China are an important contributor to the global NH3 budget. In this study, by estimating total nitrogen (N) intake based on herd structures and excreted N, a mass balance model was used to estimate NH3 losses from animal housing and manure storage facilities of dairy cattle, beef cattle, pigs, broiler and layer productions within animal farm gate and their corresponding NH3 emission intensities on the basis of animal products, N and protein in animal products. In 2009, NH3 emissions from pigs, layers, beef and dairy cattle and broiler production systems in China were 1.23, 0.52, 0.24, 0.21 and 0.09 million tons, respectively. The NH3 emission intensities were 26.6 g NH3-N kg-1 of pork, 28.1 g NH3-N kg-1 of layer eggs, 39.4 g NH3-N kg-1 of beef meat, 6.0 g NH3-N kg-1 of dairy milk and 4.6 g NH3-N kg-1 of chicken meat, or 1260 (pigs), 1514 (layers), 1297 (beef), 1107 (dairy) and 123 g NH3-N (broilers) kg-1 N in animal products. Of the sectors of NH3 emission, manure storage facilities and farmyard manure (FYM) in animal housing were the major contributors to the total NH3 emissions except for layers; housing emissions from slurry were also major contributors for dairy and pig production.

  11. Expression of the secreted FAD-dependent sulfydryl oxidase (QSOX) in the guinea pig central nervous system.

    Science.gov (United States)

    Amiot, C; Musard, J F; Hadjiyiassemis, M; Jouvenot, M; Fellmann, D; Risold, P Y; Adami, P

    2004-06-18

    cpQSOx1 is a member of the QSOx family of proteins, expressed in the guinea pig (Cavia porcellus) and ortholog of the rat rQSOx1. In this study, in vitro experiments were conducted and showed that, as other member of this family, cpQSOx1 has a sulfydryl oxidase activity, and is a secreted protein. Then, the expression of this enzyme was researched in the guinea pig brain, as very little information exists yet on the expression of QSOx family members in the central nervous system. By immunohistochemistry, RT-PCR and in situ hybridization, cpQSOx1 is synthesized by neurons throughout the whole guinea pig central nervous system. Reticular structures as the basal forebrain, reticular thalamic nucleus and reticular nuclei of the brainstem contained the densest labeling. These results are discussed in terms of putative roles of this protein in synaptic strengthening and in redox activities.

  12. Processed Products Database System

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Collection of annual data on processed seafood products. The Division provides authoritative advice, coordination and guidance on matters related to the collection,...

  13. Monitoring of behavior using a video-recording system for recognition of Salmonella infection in experimentally infected growing pigs.

    Science.gov (United States)

    Ahmed, S T; Mun, H-S; Yoe, H; Yang, C-J

    2015-01-01

    Behavior is one of the most commonly used indicators of illness; however, few studies have investigated how different common diseases affect animal behavior. This experiment was conducted to investigate behavioral and clinical alterations in growing pigs experimentally infected with Salmonella spp. during a 4-week post-infection period. A total of 48 growing pigs were divided into one of the three treatment groups (1) control, (2) infection with Salmonella Typhimurium or (3) infection with Salmonella Enteritidis. Individual pigs' behavior was recorded daily (0900 to 1100 and 1600 to 1800 h) using a video-recording system. Pigs in both infected groups had lower weight gain and feed intake during week 0 to 2 and 0 to 4 experimental period. Bacteriological data revealed that pigs in both infected groups persistently shed bacteria throughout the period of study. Oral infection of growing pigs with S. Typhimurium and S. Enteritidis significantly reduced the frequency of morning large (except week 1) and small movement throughout the study period. In the evening, significantly lowest frequency of movements were observed in the S. Enteritidis-infected group compared with the control. The standing and sitting frequency were significantly lower in both infected groups only at the morning of week 4. Infection with Salmonella spp. led to a significant reduction in the frequency and duration of morning eating and drinking throughout the experimental period, with the exception of 4th week drinking duration. The lowest frequency of evening eating during week 1 and 4 was recorded in both infected groups; whereas, the duration differed only at week 1. The evening drinking frequency only tended to decrease in response to S. Typhimurium infection at week 1. This study shows that, pigs infected with Salmonella spp. had poor performance, shedding high levels of Salmonella with their feces and reduced feeding and drinking activity, which are adaptive responses to infection and may help

  14. Production of High Potency Anti-Teschen Disease Serum From Rabbits and Guinea Pigs.

    Science.gov (United States)

    Dardiri, A H; Delay, P D

    1964-01-01

    An antiserum of high antibody content against Teschen disease Konratice virus was obtained by inoculating rabbits and guinea pigs with an antigen composed of aluminum gel and virus propagated in swine kidney cell cultures. The rabbit and guinea pig serums neutralized 1,500 TCID(50) of virus at dilutions of 1:5,120 and 1:2,048, respectively. The antibody level in the rabbit serum was tenfold greater than that in convalescent swine serum. Rabbit serum neutralized 2.8 x 10(6) plaque-forming units of the Konratice virus. At a dilution of 1:5,120, this serum neutralized 1,500 TCID(50) of the Reporyje virus. The methods used to prepare and assay the serum are described.

  15. Combined production of free-range pigs and energy crops – animal behaviour and crop damages

    DEFF Research Database (Denmark)

    Horsted, Klaus; Kongsted, Anne Grete; Jørgensen, Uffe

    2012-01-01

    to six paddocks, which, in turn, were divided into high and low-density paddocks of 700 m2 and 2200 m2, i.e. 117 and 367 m2 per pig respectively. All paddocks were divided into seven different zones representing two zones with willow, two with grass, and one each with Miscanthus, cut Miscanthus (stubs...... or season was found. Damage to the crops was very limited, even though most of the new Miscanthus shoots were eaten by the pigs in spring. Significantly more damage was caused to willow roots in high-density compared to low-density paddocks, but only in spring. Soil was found to be more turned over...

  16. Production Systems and Supplier Selection

    DEFF Research Database (Denmark)

    Pedraza-Acosta, Isabel; Pilkington, Alan; Barnes, David

    2016-01-01

    Purpose: This paper presents a combined multi-phase supplier selection model. The process repeatedly revisits the criteria and sourcing decision as the development process continues. This enables a structured adoption of product and production system innovation from strategic suppliers, where...... strategic stamping suppliers. Findings: Our contribution is the multi-phased production and product innovation process. This is an advance from traditional supplier selection and also an extension of ideas of supplier-located product development as it includes production system development, and complements...... the literature on working with strategic suppliers. Specifically, we explicitly articulate the previously unreported issue of whether a supplier chosen for its innovation capabilities at the start of the new product development process will also be the most appropriate supplier during the production system...

  17. Environmental, economic and social analysis of peri-urban pig production

    NARCIS (Netherlands)

    Wei, S.; Bai, Z.H.; Qin, W.; Xia, L.J.; Oenema, O.; Jiang, R.F.; Ma, L.

    2016-01-01

    Intensive livestock production expands rapidly around cities to meet the food demand and the improvement of people's living standard in developing countries, such as China. However, little is known about the environmental costs and socio-economic performances of these systems. Here, the performan

  18. Survey of quantitative antimicrobial consumption per production stage in farrow-to-finish pig farms in Spain

    Science.gov (United States)

    Moreno, Miguel A.

    2014-01-01

    Objectives To characterise antimicrobial use (AMU) per production stage in terms of drugs, routes of application, indications, duration and exposed animals in farrow-to-finish pig farms in Spain. Design Survey using a questionnaire on AMU during the six months prior to the interview, administered in face-to-face interviews completed from April to October 2010. Participants 108 potentially eligible farms covering all the country were selected using a multistage sampling methodology; of these, 33 were excluded because they did not fulfil the participation criteria and 49 were surveyed. Results The rank of the most used antimicrobials per farm and production stage and administration route started with polymyxins (colistin) by feed during the growing and the preweaning phases, followed by β-lactams by feed during the growing and the preweaning phases and by injection during the preweaning phase. Conclusions The study demonstrates that the growing stage (from weaning to the start of finishing) has the highest AMU according to different quantitative indicators (number of records, number of antimicrobials used, percentage of farms reporting use, relative number of exposed animals per farm and duration of exposure); feed is the administration route that produces the highest antimicrobial exposure based on the higher number of exposed animals and the longer duration of treatment; and there are large differences in AMU among individual pig farms. PMID:26392868

  19. Effect of different cooking methods on lipid oxidation and formation of free cholesterol oxidation products (COPs) in Latissimus dorsi muscle of Iberian pigs.

    Science.gov (United States)

    Broncano, J M; Petrón, M J; Parra, V; Timón, M L

    2009-11-01

    The aim of this work was to study the influence of different cooking methods (grilled (GR), fried (FP), microwave (MW) and roasted (RO)) on lipid oxidation and formation of free cholesterol oxidation products (COPs) of meat from Iberian pigs that have been fed on an intensive system. Moisture and total lipid content, TBARs, hexanal and COPs were measured in Latissimus dorsi muscle samples. Cooking did not produce changes in total lipid content in meat but induced significantly higher lipid oxidation (TBARs and hexanal values) (p<0.001) and cholesterol oxidation (COPs) (p<0.01). When the different cooking methods were studied, the grilled method was the least affected by lipid oxidation (TBARs and hexanal) compared to the others. There were no significant differences among different cooking methods on COPs values. The most abundant cholesterol oxides were both 7α-hydroxycholesterol and 7β-hydroxycholesterol in all groups studied.

  20. A metabolic system-wide characterisation of the pig: a model for human physiology.

    Science.gov (United States)

    Merrifield, Claire A; Lewis, Marie; Claus, Sandrine P; Beckonert, Olaf P; Dumas, Marc-Emmanuel; Duncker, Swantje; Kochhar, Sunil; Rezzi, Serge; Lindon, John C; Bailey, Mick; Holmes, Elaine; Nicholson, Jeremy K

    2011-09-01

    The pig is a single-stomached omnivorous mammal and is an important model of human disease and nutrition. As such, it is necessary to establish a metabolic framework from which pathology-based variation can be compared. Here, a combination of one and two-dimensional (1)H and (13)C nuclear magnetic resonance spectroscopy (NMR) and high-resolution magic angle spinning (HR-MAS) NMR was used to provide a systems overview of porcine metabolism via characterisation of the urine, serum, liver and kidney metabolomes. The metabolites observed in each of these biological compartments were found to be qualitatively comparable to the metabolic signature of the same biological matrices in humans and rodents. The data were modelled using a combination of principal components analysis and Venn diagram mapping. Urine represented the most metabolically distinct biological compartment studied, with a relatively greater number of NMR detectable metabolites present, many of which are implicated in gut-microbial co-metabolic processes. The major inter-species differences observed were in the phase II conjugation of extra-genomic metabolites; the pig was observed to conjugate p-cresol, a gut microbial metabolite of tyrosine, with glucuronide rather than sulfate as seen in man. These observations are important to note when considering the translatability of experimental data derived from porcine models.

  1. Impact of immune system stimulation on the ileal nutrient digestibility and utilisation of methionine plus cysteine intake for whole-body protein deposition in growing pigs.

    Science.gov (United States)

    Rakhshandeh, Anoosh; Htoo, John K; Karrow, Neil; Miller, Stephen P; de Lange, Cornelis F M

    2014-01-14

    The impact of immune system stimulation (ISS) on the ileal nutrient digestibility and utilisation of dietary methionine plus cysteine (SAA) intake for whole-body protein deposition (PD) was evaluated in growing pigs. For this purpose, sixty barrows were used in two experiments: thirty-six pigs in Expt I and twenty-four pigs in Expt II. Pigs were feed restricted and assigned to five levels of dietary SAA allowance (three and two levels in Expt I and II, respectively) from SAA-limiting diets. Following adaptation, pigs at each dietary SAA level were injected with either increasing amounts of Escherichia coli lipopolysaccharide (ISS+; eight and six pigs per dietary SAA level in Expt I and II, respectively) or saline (ISS - ; four and six pigs in Expt I and II, respectively) while measuring the whole-body nitrogen (N) balance. After N-balance observations, pigs were euthanised, organs were removed and ileal digesta were collected for determining nutrient digestibility. Ileal digestibility of gross energy, crude protein and amino acids was not affected by ISS (P>0·20). ISS reduced PD at all levels of dietary SAA intake (P0·20). Physiological and metabolic changes associated with systemic ISS had no effect on the ileal digestibility of nutrients per se, but altered SAA requirements for PD in growing pigs.

  2. Central activation of the sympathetic nervous system including the adrenals in anaesthetized guinea pigs by the muscarinic agonist talsaclidine.

    Science.gov (United States)

    Walland, A; Pieper, M P

    1998-04-01

    Talsaclidine, a novel M1-receptor selective muscarinic agonist for cholinergic substitution therapy of Alzheimer's disease, activates the sympathetic nervous system in guinea pigs and dogs at the orthosympathic ganglia and the paraganglionic adrenals. Results from guinea pigs provide indirect evidence for an additional central site of action. The present investigation in anaesthetized and vagotomized guinea pigs intended to demonstrate central activation of the sympathetic nervous system directly by comparing the blood pressure effects of intracerebroventricular and intravenous injections of small doses of talsaclidine. Increasing doses of 0.2 and 0.6 mg/kg talsaclidine were injected alternately into the third cerebral ventricle and intravenously in 6 guinea pigs before and after blockade of peripheral muscarinic receptors with 1 mg/kg ipratropium bromide i.v. In another group of 6 animals the injections were given into the cisterna cerebellomedullaris using the same protocol. In both groups central administration of talsaclidine caused dose-related hypertension while intravenous injections were hypotensive. Ipratropium bromide, a peripheral antimuscarinic drug, reversed this hypotensive action of intravenous talsaclidine into hypertension, but did not inhibit the effects of central administration. In contrast, atropine, an antimuscarinic drug which passes the blood-brain barrier, abolished the effect of 0.6 mg/kg talsaclidine injected into the cisterna cerebellomedullaris of 8 guinea pigs. The hypertensive effect of a first injection of 0.6 mg/kg talsaclidine into the cisterna cerebellomedullaris of 6 guinea pigs was approximately twice as large as that of a second given 90 min after bilateral adrenalectomy. Sham operation in another 6 animals was not inhibitory. The results demonstrate that talsaclidine, a selective muscarinic M1-receptor agonist, activates central parts of the sympathetic nervous system, including central projections of the adrenals by an action

  3. Verification of good production practices that reduce the risk of exposure of pigs to Trichinella

    Directory of Open Access Journals (Sweden)

    Gamble H.R.

    2001-06-01

    Full Text Available Control of Trichinella infection in swine has traditionally been accomplished by inspection of individual carcasses or by post-slaughter processing to inactivate parasites. Recent declines in prevalence of this parasite in domestic swine, coupled with improvements in swine management systems, offer the opportunity to document pork safety during the production phase. We report here on a certification pilot study using an audit to document good production practices for swine relative to the risk of exposure to trichinae. Based on the results, improvements in the program have been made and further studies will be undertaken prior to launching a voluntary trichinae herd certification program in the United States.

  4. GLOBAL PRODUCTION SYSTEM

    Directory of Open Access Journals (Sweden)

    Dumitru FILIPEANU

    2016-07-01

    Full Text Available The most significant transformations that globalization produces occur in production. Since the '60s, a new division of labor has made its presence felt in the world, arising from the "de-industrialization" of the developed and transfer production capacity of resource-intensive industries and pollutants from these countries to the developing world. "Dislocation" industry had the interim foreign direct investment made in the new industrialized countries, the latter becoming, in turn, sources of direct foreign investment, taking its capital in other countries in developing handsets. Currently, FDI destination is no longer a priority in developing countries, yet they are increasingly leaning towards the developed countries, due to the attractiveness offered by their economies.

  5. Village poultry production system

    OpenAIRE

    Wondmeneh, E.; Waaij, van der, Liesbeth; Udo, H.M.J.; Tadelle, D.; van Arendonk,

    2016-01-01

    This study identified perception of poultry farmers’ on impact of interventions in village poultry production and quantified the impacts of interventions on flock and economic performance using modelling. A structured questionnaire was used to collect data on perceptions of poultry keeping and performances from 240 randomly selected households in two districts of Ethiopia. Crop was the major source of income, and poultry generated supplementary income. Farmers perceived that demand and price ...

  6. Sero-prevalence of Taenia spp. cysticercosis in rural and urban smallholder pig production settings in Uganda.

    Science.gov (United States)

    Kungu, Joseph M; Dione, Michel M; Ejobi, Francis; Harrison, Leslie J S; Poole, E Jane; Pezo, Danilo; Grace, Delia

    2017-01-01

    The pork tapeworm, Taenia solium, is prevalent in Uganda although the prevalence has not been determined in all areas of the country. A cross-sectional study, to determine the sero-prevalence of the parasite in pigs kept under rural and urban production settings, was carried out in three Ugandan districts, Masaka, Mukono and Kamuli. Serum samples from 1185 pigs were tested for the presence of T. solium cysticercosis antigen using the HP10 antigen-ELISA (Ag-ELISA) and the ApDia Ag-ELISA assays. Using parallel interpretation of the two tests showed lower levels of observed prevalence of T. solium in rural production settings (10.8%) compared to urban (17.1%). Additionally, Maximum Likelihood Estimation for evaluating assays in the absence of a gold standard, using TAGS on the R platform, estimated the true sero-prevalence to be lower in rural production setting, 0.0% [0.0-3.2%; 95% confidence interval (CI)] than in urban production setting, 12.3% (4.2-77.5% CI). When the sensitivity/specificity (Se/Sp) of the assays were estimated, assuming conditional independence of the tests, HP10 Ag-ELISA was more sensitive and specific [(Se=53.9%; 10.1-100% CI), (Sp=97.0%; 95.9-100% CI)] than the ApDia assay [(Se=20.2%; 1.5-47.7% CI), (Sp=92.2%; 90.5-93.9% CI)]. Subject to parasitological verification, these results indicate there may be a need to implement appropriate control measures for T. solium in the study areas.

  7. Growth performance of early-weaned pigs is enhanced by feeding epidermal growth factor-expressing Lactococcus lactis fermentation product.

    Science.gov (United States)

    Bedford, Andrea; Huynh, Evanna; Fu, Molei; Zhu, Cuilan; Wey, Doug; de Lange, Cornelis; Li, Julang

    2014-03-10

    We have previously generated epidermal growth factor expressing Lactococcus lactis (EGF-LL) using bioengineering approach, and shown that feeding newly-weaned piglets EGF-LL improves digestive function. To address concerns over the use of genetically modified organisms (GMO), the objective of the current study was to investigate the effect of feeding the EGF-LL fermentation product, after removal of the genetically modified EGF-LL, on growth performance and intestine development of newly-weaned piglets. One hundred and twenty newly-weaned piglets were fed ad libitum according to a 2-phase feeding program. Four pens were assigned to each of three treatments: (1) complete EGF-LL fermentation product (Ferm), (2) supernatant of EGF-LL fermentation product, after removal of EGF-LL (Supern), or (3) blank M17GE media (Control). EGF-LL or its fermented supernatant was administrated to piglets in the first 3 weeks post-weaning; their growth performance was monitored throughout treatment, and for the following week. Daily body weight gain (254.8g vs. 200.5g) and Gain:Feed (0.541kg/kg vs. 0.454kg/kg) of pigs on the Supern group were significantly improved compared to that of Control, although no difference was observed between the Ferm and Control pigs. Intestinal sucrase activity was increased in Supern- compared to Control group (166.3±62.1 vs. 81.4±56.5nmol glucose released/mg protein; PGMO-free EGF-LL fermentation product is effective in increasing growth performance of early-weaned piglets. Copyright © 2014 Elsevier B.V. All rights reserved.

  8. Diurnal variation in insulin-stimulated systemic glucose and amino acid utilization in pigs fed with identical meals at 12-hour intervals

    NARCIS (Netherlands)

    Koopmans, S.J.; Meulen, van der J.; Dekker, R.A.; Corbijn, H.; Mroz, Z.

    2006-01-01

    The diurnal variation in insulin-stimulated systemic glucose and amino acid utilization was investigated in eleven pigs of similar to 40 kg. Pigs were fed isoenergetic/isoproteinic diets (366kj/kg BW0.75 per meal) in two daily rations (06:00 and 18:00h). After a 3-week habituation period, hyperinsul

  9. Production control system specified quality sausage products

    Directory of Open Access Journals (Sweden)

    A. V. Tokarev

    2016-01-01

    Full Text Available The problem of management of production target in technological system of production of sausages of the set quality is considered in article. Decomposition of technological system is considered. Functions of management are allocated: formation of an optimum compounding of forcemeat, expert analysis of a compounding, laboratory analysis of a compounding and its statement. Information technology of interaction of these functions is offered. The mathematical problem definition of finding of an optimum compounding meat product with use of possible substitutes of ingredients is presented. This mathematical problem is a classical linear programming problem whose solution has the standard program. Since the manufacture of the finished product are various nonlinear effects are taken into account at the present time it is practically impossible, the methodology provided in this operation "Expert analysis of the formulation" and "Laboratory analysis of the finished product." An example of calculating the optimum alternative base recipe "Sausages “Viennese with cheese”" TS 9213-010-40155161-2002. For an alternative formulation demands were made at a cost of meat, the ingredient composition, as well as the final product organoleptic and physic-chemical indicators should comply with regulatory requirements "Sausages “Viennese with cheese”" TS 9213-010-40155161-2002. Indicator acid activity (pH calculated stuffing formulation should be in the range 6.0-6.3. As a partial replacement for the main raw material have been proposed acceptable substitutes. It was necessary to calculate on the basis of the formulation "Sausages “Viennese with cheese”" TS 9213-010-40155161-2002 optimal price and quality alternative formulation. As a result of depreciation of the value of alternative stuffing recipe was 14,5 % when all of the restrictions on the consumer properties. The proposed information technology implemented in the software package "Multi

  10. Programming Pig

    CERN Document Server

    Gates, Alan

    2011-01-01

    This guide is an ideal learning tool and reference for Apache Pig, the open source engine for executing parallel data flows on Hadoop. With Pig, you can batch-process data without having to create a full-fledged application-making it easy for you to experiment with new datasets. Programming Pig introduces new users to Pig, and provides experienced users with comprehensive coverage on key features such as the Pig Latin scripting language, the Grunt shell, and User Defined Functions (UDFs) for extending Pig. If you need to analyze terabytes of data, this book shows you how to do it efficiently

  11. Effects of Residual Hypochlorite Ion on Methane Production during the Initial Anaerobic Digestion Stage of Pig Slurry.

    Science.gov (United States)

    Yoon, Young-Man; Kim, Hyun-Cheol; So, Kyu-Ho; Kim, Chang-Hyun

    2013-01-01

    The hypochlorite ion (OCl(-)) is a widely used disinfecting agent in pig rearing in Korea, but its residual effect on CH4 production from pig slurry is unclear. The objective of this study was to investigate the inhibition effects of residual OCl(-) on CH4 production during the initial anaerobic digestion stage of pig slurry. Three organic concentrations (9.9, 26.2 and 43.7 g/L) of volatile solids (VS) were tested with the addition of 52.3 mg/L OCl(-), ten times of the typical concentration used in Korea, or without OCl(-) (Control) in anaerobic batch culture. The culture was run under mesophilic (38°C) conditions for 20 d. At the lowest organic concentration with OCl(-), the VS degradation was 10.3% lower (pOCl(-), it did not differ from Control. CH4 yields were higher in the control treatments than their OCl(-) counterpart cultures, and CH4 yields of Control and OCl(-) treatments at the organic concentrations of 9.9, 26.2 and 43.7 g/L differed in the probability level (p) of 0.31, 0.04, and 0.06, respectively. Additionally, CH4 concentration increased steeply and reached 70.0% within 4 d in the absence OCl(-), but a gradual increase up to 60.0% was observed in 6 d in the OCl(-) treated cultures. The Rm (the maximum specific CH4 production rate) and λ (lag phase time) of 9.9 g/L with OCl(-) were 8.1 ml/d and 25.6 d, while the Rm was increased to 15.1 ml/d, and λ was reduced to 11.4 d in PS-III (higher organic concentration) with OCl(-). The results suggest that a prolonged fermentation time was necessary for the methanogens to overcome the initial OCl(-) inhibitory effect, and an anaerobic reactor operated with high organic loadings was more advantageous to mitigate the inhibitory effect of residual hypochlorite ion.

  12. The system Around the Product

    DEFF Research Database (Denmark)

    Morelli, Nicola

          The design discipline has traditionally focused on products physical aspects, giving for granted that the context of services, infrastructures and technology will support the product life. A systemic perspective would revise such a focus, in order to project design competences over systemic...... and methods to handle immaterial aspects such as time sequences, actors? role and logical links in a product service system (PSS). Tools and methods can be borrowed from other disciplinary contexts, their relevance, though, derives from their adaptation to this specific study area: designing in a systemic...... aspects usually neglected by designers. This perspective shift is a challenge for designers, who have very efficient methods and tools to handle physical aspects of product design, but need to define an ?operative paradigm? to operate in a systemic context. An operative paradigm consists of a set of tools...

  13. Dual purpose system that treats anaerobic effluents from pig waste and produce Neochloris oleoabundans as lipid rich biomass.

    Science.gov (United States)

    Olguín, Eugenia J; Castillo, Omar S; Mendoza, Anilú; Tapia, Karla; González-Portela, Ricardo E; Hernández-Landa, Víctor J

    2015-05-25

    Dual purpose systems that treat wastewater and produce lipid rich microalgae biomass have been indicated as an option with great potential for production of biodiesel at a competitive cost. The aim of the present work was to develop a dual purpose system for the treatment of the anaerobic effluents from pig waste utilizing Neochloris oleoabundans and to evaluate its growth, lipid content and lipid profile of the harvested biomass and the removal of nutrients from the media. Cultures of N. oleoabundans were established in 4 L flat plate photobioreactors using diluted effluents from two different types of anaerobic filters, one packed with ceramic material (D1) and another one packed with volcanic gravel (D2). Maximum biomass concentration in D1 was 0.63 g L(-1) which was significantly higher than the one found in D2 (0.55 g L(-1)). Cultures were very efficient at nutrient removal: 98% for NNH4(+) and 98% for PO4(3-). Regarding total lipid content, diluted eflluents from D2 promoted a biomass containing 27.4% (dry weight) and D1 a biomass containing 22.4% (dry weight). Maximum lipid productivity was also higher in D2 compared to D1 (6.27±0.62 mg L(-1) d(-1) vs. 5.12±0.12 mg L(-1) d(-1)). Concerning the FAMEs profile in diluted effluents, the most abundant one was C18:1, followed by C18:2 and C16:0. The profile in D2 contained less C18:3 (linolenic acid) than the one in D1 (4.37% vs. 5.55%). In conclusion, this is the first report demonstrating that cultures of N. oleoabundans treating anaerobic effluents from pig waste are very efficient at nutrient removal and a biomass rich in lipids can be recovered. The maximum total lipid content and the most convenient FAMEs profile were obtained using effluents from a digester packed with volcanic gravel. Copyright © 2015 Elsevier B.V. All rights reserved.

  14. Effect of the estrogen receptor locus on reproduction and production traits in four commercial pig lines.

    Science.gov (United States)

    Short, T H; Rothschild, M F; Southwood, O I; McLaren, D G; de Vries, A; van der Steen, H; Eckardt, G R; Tuggle, C K; Helm, J; Vaske, D A; Mileham, A J; Plastow, G S

    1997-12-01

    We investigated the effect of the estrogen receptor (ESR) gene on growth and reproductive traits in four Large White-based commercial pig lines. A total of 9,015 litter records from 4,262 sows genotyped at the ESR locus were analyzed to determine whether ESR influenced total number born (TNB) or number born alive (NBA). Teat number (TN), test ADG, ADFI, feed:gain ratio (F/G), and ultrasonic backfat (BF) were also analyzed to determine effects of ESR. The TNB and NBA were increased per favorable allele of ESR (P NBA, respectively, in later parities (P .10), although ADF was reduced 18 g/d per copy of the favorable litter size allele (P < .05). Average TN was 13.1 for pigs carrying the favorable litter size allele vs 13.2 for noncarriers (P < .05). Marker-assisted selection using ESR is warranted to increase litter size in the Large White-based lines considered here and will be of considerable economic value to pork producers.

  15. Nonrenewable Energy Cost and Greenhouse Gas Emissions of a “Pig-Biogas-Fish” System in China

    OpenAIRE

    2012-01-01

    The purpose of this study is to assess the energy savings and emission reductions of the present rural biogas system in China. The life cycle assessment (LCA) method is used to analyze a “pig-biogas-fish” system in Jingzhou, Hubei Province, China. The nonrenewable energy cost and the greenhouse gas (GHG) emissions of the system, including the pigsty, the biogas digester, and the fishpond, are taken into account. The border definition is standardized because of the utilization of the database ...

  16. Distribution of capsular types and production of muramidase-released protein (MRP) and extracellular factor (EF) of Streptococcus suis strains isolated from diseased pigs in seven European countries

    NARCIS (Netherlands)

    Wisselink, H.J.; Smith, H.E.; Stockhofe-Zurwieden, N.; Peperkamp, K.; Vecht, U.

    2000-01-01

    Streptococcus suis strains (n=411), isolated from diseased pigs in seven European countries were serotyped using specific antisera against serotype 1 to 28, and were phenotyped on the basis of their muramidase-released-protein (MRP) and extracellular-factor protein (EF) production. Overall, S. suis

  17. Differential gene regulation of steroidogenic transcripts and estradiol production following in vitro pig embryo elongation in alginate hydrogel three-dimensional matrix

    Science.gov (United States)

    Between d 10 and 12 of gestation, the pig embryo elongates from a sphere to a long thin, filament. During this time, the embryo increases the production of estrogen via an increase in steroidogenic transcripts, which is critical for maternal recognition of pregnancy. To date, attempts to elongate po...

  18. Investment and export opportunities in a sustainable pig supply chain in China

    NARCIS (Netherlands)

    Hoste, R.; Hu, D.; Tolkamp, J.

    2013-01-01

    Given the increasing average income of the Chinese consumer, increasing awareness of food safety and giant performance arrears there is a large need for investments in the entire pig supply chain. The Netherlands can contribute to a more sustainable Chinese pig production system, both by exporting

  19. Investment and export opportunities in a sustainable pig supply chain in China

    NARCIS (Netherlands)

    Hoste, R.; Hu, D.; Tolkamp, J.

    2013-01-01

    Given the increasing average income of the Chinese consumer, increasing awareness of food safety and giant performance arrears there is a large need for investments in the entire pig supply chain. The Netherlands can contribute to a more sustainable Chinese pig production system, both by exporting p

  20. Epidemiological analysis of the dynamic and diversity of Salmonella spp. in five German pig production clusters using pheno- and genotyping methods: an exploratory study.

    Science.gov (United States)

    Niemann, J; Tietze, E; Ruddat, I; Fruth, A; Prager, R; Rabsch, W; Blaha, T; Münchhausen, C; Merle, R; Kreienbrock, L

    2015-03-23

    An exploratory study in five conventional pig production clusters was carried out to investigate the dynamic and diversity of Salmonella spp. within different production stages and sample site categories (pooled feces, direct and non-direct environment). Observing two production cycles per production cluster, a total of 1276 samples were collected along the pig production chain. Following a microbiological examination via culture, 2246 subcultures were generated out of 285 Salmonella positive samples and analysed by pheno- and genotyping methods. Based on a combination of serotyping, MLVA (multiple-locus variable-number tandem repeat (VNTR) analysis), PFGE (pulse-field gel electrophoresis) and MLST (multilocus sequence typing), an amount of 22.3% Salmonella positive samples were characterized in clonal lineages and its variants. Within each production cluster, one main clonal lineage could be identified and persisted over both production cycles with a large diversity of variants and a wide distribution in sample site categories and production stages. Results underline the importance of biosecurity with emphasis on the environment to prevent persistence and circulation of Salmonella within herds. Furthermore, the combined implementation of MLVA, PFGE and MLST with conventional culture techniques for isolate classification could be successfully applied as an effective and valuable tool for identifying similar pattern of Salmonella occurrence within pig production clusters.

  1. Investigation of the disposal of dead pigs by pig farmers in mainland China by simulation experiment.

    Science.gov (United States)

    Wu, Linhai; Xu, Guoyan; Li, Qingguang; Hou, Bo; Hu, Wuyang; Wang, Jianhua

    2017-01-01

    Dead pigs are a major waste by-product of pig farming. Thus, safe disposal of dead pigs is important to the protection of consumer health and the ecological environment by preventing marketing of slaughtered and processed dead pigs and improper dumping of dead pigs. In this study, a probability model was constructed for the disposal of dead pigs by pig farmers by selecting factors affecting disposal. To that end, we drew on the definition and meaning of behavior probability based on survey data collected from 654 pig farmers in Funing County, Jiangsu Province, China. Moreover, the role of influencing factors in pig farmers' behavioral choices regarding the disposal of dead pigs was simulated by simulation experiment. The results indicated that years of farming had a positive impact on pig farmers' choice of negative disposal of dead pigs. Moreover, there was not a simple linear relationship between scale of farming and pig farmers' behavioral choices related to the disposal of dead pigs. The probability for farmers to choose the safe disposal of dead pigs increased with the improvement of their knowledge of government policies and relevant laws and regulations. Pig farmers' behavioral choice about the disposal of dead pigs was also affected by government subsidy policies, regulation, and punishment. Government regulation and punishment were more effective than subsidy. The findings of our simulation experiment provide important decision-making support for the governance in preventing the marketing of dead pigs at the source.

  2. Intra-unit correlations in seroconversion to Actinobacillus pleuropneumoniae and Mycoplasma hyopneumoniae at different levels in Danish multi-site pig production facilities

    DEFF Research Database (Denmark)

    Vigre, Håkan; Dohoo, I.R.; Stryhn, H.;

    2004-01-01

    2) and Mycoplasma hyopneumoniae (Mh). Based on the estimated variances, three newly described computational methods (model linearisation, simulation and linear modelling) and the standard method (latent-variable approach) were used to estimate the correlations (intra-class correlation components......, ICCs) between pigs in the same production unit regarding seroconversion. Substantially different values of ICCs were obtained from the four methods. However, ICCs obtained by the simulation and the model linearisation were quite consistent. Data used for estimation were collected from 1161 pigs from...

  3. Composition and biogas yield of a novel source segregation system for pig excreta

    NARCIS (Netherlands)

    Vu, Phuong T.; Melse, Roland W.; Zeeman, Grietje; Groot Koerkamp, Peter W.G.

    2016-01-01

    The performance of a novel source segregation method for pig excreta (a V-shaped conveyor belt underneath the slatted pen floor) was compared to conventional separation methods for pig slurry (screw press, centrifugation, flocculation with/without centrifugation). For the source segregation

  4. Composition and biogas yield of a novel source segregation system for pig excreta

    NARCIS (Netherlands)

    Vu, Phuong T.; Melse, Roland W.; Zeeman, Grietje; Groot Koerkamp, Peter W.G.

    2016-01-01

    The performance of a novel source segregation method for pig excreta (a V-shaped conveyor belt underneath the slatted pen floor) was compared to conventional separation methods for pig slurry (screw press, centrifugation, flocculation with/without centrifugation). For the source segregation syste

  5. Accumulation of glycation products in. cap alpha. -H pig lens crystallin and its bearing to diabetic cataract genesis

    Energy Technology Data Exchange (ETDEWEB)

    Vidal, P.; Cabezas-Cerrato, J.

    1988-01-01

    The incorporation of /sup 11/C-glucose in native pig crystalline by in vitro incubation was found, after subsequent dialysis, to affect all 5 classes of crystallin separated by Sepharose CL-6B column chromatography. Though the radioactivity of the ..cap alpha..-H fraction was three times greater than that of any of the others, autoradiographs of SDS-PAGE gels showed /sup 11/C-glucose adducts to be present in all soluble protein subunits, without there being any evidence of preferential glycation of the ..cap alpha..-H subunits. The concentration of stable glycation products in the ..cap alpha..-H chromatographic fraction of soluble crystallins is suggested to be due the addition of glycated material to this fraction as result of glycation-induced hyperaggregation, and not because the ..cap alpha..-H subunits were especially susceptible to glycation.

  6. Analysis of single nucleotide polymorphisms in major and candidate genes for production traits in Nero Siciliano pig breed

    Directory of Open Access Journals (Sweden)

    Alessandro Zumbo

    2010-01-01

    Full Text Available Nero Siciliano (NS; Sicilian Black is a local pig breed reared on the island of Sicily mainly under extensive management.The breed is well adapted to marginal conditions and is appreciated for its reproductive performance, disease resistanceand production of tasty meat. For a genetic characterization of this breed we analyzed the allele frequencies of singlenucleotide polymorphisms (SNPs in eight major or candidate genes (ryanodine receptor 1, RYR1; Na+, K+ ATPase subunitα 2, ATP1A2; myosin heavy chain 2B, MYH4; sarcolipin, SLN; cathepsin B, CTSB; cystatin B, CSTB; estrogen receptor,ESR; melanocortin receptor 1, MC1R for performance and phenotypic traits. The animals that were sampled andanalyzed represent about 6-8% of the total NS pig population. PCR-RFLP or PCR-SSCP techniques were used to type theDNA markers in the selected loci. Exact test of Hardy-Weinberg equilibrium was computed for each locus, Fis statisticsand heterozygosity were calculated for each locus and over all loci. Allele frequencies obtained in NS breed were comparedto the frequencies already available in literature for the Large White, Landrace, Duroc, Belgian Landrace, Piétrain,Hampshire and Meishan breeds. For the ESR locus, as no information on the distribution of the two alleles were available,we typed a sample of unrelated pigs from the considered breeds.Even if only eight loci were studied in NS breed, important elements were obtained from the data. The 1843T (n alleleat the RYR1 locus is present in NS breed, thus the molecular test to identify the carriers of this allele should be adoptedto avoid its spreading in the population. Moreover, other studies are needed to clarify the allelic structure of the MC1Rgene, which affects coat color, in order to evaluate if this gene could be used in genetic tests for the traceability of themeat products of this breed. Finally, the present work represents an attempt to evaluate data on mutations within majorand candidate genes

  7. Risk factors associated with swine dysentery in East-European pig production units

    Directory of Open Access Journals (Sweden)

    Mirko C.P.

    2005-01-01

    Full Text Available The objective of the present study was to determine the risk factors for swine dysentery in East-European middle-size to large farrow to finish units with separate breeding and grower-finisher facilities. Ten breeding animals (3-10% of the female inventory and 10 grower tinisher pigs (80-140 days-of age were sampled in each herd for polymerase chain reaction testing (PCR for Brachyspira hyodysenteriae (B hyo in their feces. Of 139 farrow to finish units, 51 (36.7% were positive, 49 (35,3% were negative, and 39 (28.1% were inconclusive for B hyo by PCR. In breeding subunits, twelve variables passed the screening criterion for risk factors (P<.2 for B hyo PCR positivity. The odds of the breeding subunits being B hyo PCR positive were 3.5 times greater when the grower-finisher subunit was positive and the fiber content of the diet was >6%. Use of "all in all out" farrowing policy and having >60% multiparous sows, each reduced the odds of being B hyo PCR positive about fourfold. In growing-finishing subunits, fourteen variables passed the screening criterion for risk factors (P<-2 for B hyo PCR positivity. B hyo PCR positive status of the breeding subunits and higher fiber content of the diet were the most influential variable, with the odds of the grower-finisher subunits being B hyo PCR positive almost eight times greater when the breeding subunit was also B hyo PCR positive. Grower-finisher B hyo PCR positivity was also associated with the percentage of pigs housed on concrete slats, with the odds of being positive 7.5 times higher for subunits where more that 70% of the animals were kept on concrete slats compared to all other floor types. There was a strong association between grower-finisher status and whether the animals were in outdoor lots with the odds of being B hyo PCR positive substantially lower for pigs in outdoor lots compared with all other surfaces.

  8. Glucagon-like peptides GLP-1 and GLP-2, predicted products of the glucagon gene, are secreted separately from pig small intestine but not pancreas

    DEFF Research Database (Denmark)

    Holst, J J; Poulsen, Steen Seier

    1986-01-01

    identified in glucagon-producing cells of the pancreatic islets, and in glicentin-producing cells of the small intestine. Immunoreactive GLP-1 and 2 in intestinal extracts corresponded in molecular size to peptides synthesized according to the predicted structure. By reverse phase HPLC, intestinal......We developed specific antibodies and RIAs for glucagon-like peptides 1 and 2 (GLP-1 and GLP-2), two predicted products of the glucagon gene, and studied the occurrence, nature, and secretion of immunoreactive GLP-1 and GLP-2 in pig pancreas and small intestine. Immunoreactive GLP-1 and GLP-2 were...... and synthetic GLP-1 behaved similarly, whereas synthetic and intestinal GLP-2 differed. Pancreatic extracts contained a large peptide with both GLP-1 and GLP-2 immunoreactivity. Secretion was studied using isolated perfused pig pancreas during arginine stimulation, and isolated perfused pig ileum during either...

  9. Genetic relationships between boar feed efficiency and sow piglet production, body condition score, and stayability in Norwegian Landrace pigs.

    Science.gov (United States)

    Martinsen, K H; Ødegård, J; Aasmundstad, T; Olsen, D; Meuwissen, T H E

    2016-08-01

    Both feed efficiency and sow production are economically important traits in pig breeding. One challenge in a maternal line such as Norwegian Landrace is to breed for highly feed efficient fattening pigs and, at the same time, produce sows with high daily feed intake to maintain their BCS in multiple parities. The aim of this study was to estimate genetic correlations among novel feed efficiency measurements on Norwegian Landrace boars and piglet production, stayability, and body condition in Norwegian Landrace sows. The feed efficiency measurements were lean meat and fat efficiency. These measurements were calculated using an extended residual feed intake model where total feed intake in the test period was the response variable and fat (kg) and lean meat (kg) on the carcass were included as both fixed and random regressions. The random regression coefficients that resulted from this model were breeding values, which represented the amount of feed used to produce an extra kilogram of lean meat and fat. The sow traits were stayability of the sow from first to second parity, BCS at weaning, litter weight at 3 wk, and total number of piglets born. All traits were recorded on first parity purebred Norwegian Landrace and analyzed using multivariate animal models. All genetic correlations between fat efficiency and sow traits were low. Significant genetic correlations were found only between fat efficiency and stayability (0.21 ± 0.11) and between fat efficiency and total litter weight at 3 wk (0.21 ± 0.10). The results indicate that selection for efficient deposition of fat could give poor stayability and lower litter weight at 3 wk in first parity sows. The genetic correlations between lean meat efficiency and sow traits were not significantly different from 0 and signified no genetic relationships between these traits. Selection for efficient deposition of lean meat should not affect the sow traits and is, therefore, beneficial.

  10. Blood-Brain Barrier Disruption and Oxidative Stress in Guinea Pig after Systemic Exposure to Modified Cell-Free Hemoglobin

    Science.gov (United States)

    Butt, Omer I.; Buehler, Paul W.; D'Agnillo, Felice

    2011-01-01

    Systemic exposure to cell-free hemoglobin (Hb) or its breakdown products after hemolysis or with the use of Hb-based oxygen therapeutics may alter the function and integrity of the blood-brain barrier. Using a guinea pig exchange transfusion model, we investigated the effect of a polymerized cell-free Hb (HbG) on the expression of endothelial tight junction proteins (zonula occludens 1, claudin-5, and occludin), astrocyte activation, IgG extravasation, heme oxygenase (HO), iron deposition, oxidative end products (4-hydroxynonenal adducts and 8-hydroxydeoxyguanosine), and apoptosis (cleaved caspase 3). Reduced zonula occludens 1 expression was observed after HbG transfusion as evidenced by Western blot and confocal microscopy. Claudin-5 distribution was altered in small- to medium-sized vessels. However, total expression of claudin-5 and occludin remained unchanged except for a notable increase in occludin 72 hours after HbG transfusion. HbG-transfused animals also showed increased astrocytic glial fibrillary acidic protein expression and IgG extravasation after 72 hours. Increased HO activity and HO-1 expression with prominent enhancement of HO-1 immunoreactivity in CD163-expressing perivascular cells and infiltrating monocytes/macrophages were also observed. Consistent with oxidative stress, HbG increased iron deposition, 4-hydroxynonenal and 8-hydroxydeoxyguanosine immunoreactivity, and cleaved caspase-3 expression. Systemic exposure to an extracellular Hb triggers blood-brain barrier disruption and oxidative stress, which may have important implications for the use of Hb-based therapeutics and may provide indirect insight on the central nervous system vasculopathies associated with excessive hemolysis. PMID:21356382

  11. Combustion products from various kinds of fibers: toxicological hazards from smoke exposure. [Guinea pigs

    Energy Technology Data Exchange (ETDEWEB)

    Betol, E.; Mari, F.; Orzalesi, G.; Volpato, I.

    1983-08-15

    The smoke exposure hazards during combustion of carbon and nitrogen-containing fibers were evaluated in guinea pigs intoxicated by gradual exposure to HCN and CO neo-formed from foam rubber, wool and PAN (Polyacrylonitrile). The most prominent result of our study was that the neo-formation of HCN from 1 g of PAN was 1500 ppm, much higher than from foam rubber and wool because of the presence of many -CN groups in the polymer chemical structure. This concentration of HCN is estimated to be lethal. Extrapolating this data, a lethal concentration of HCN could be obtained by burning 2 kg of PAN in an average sized living room. The above-mentioned 1 g of PAN was burned in a 15.6-liter combustion chamber.

  12. Heat production in growing pigs calculated according to the RQ and CN methods

    DEFF Research Database (Denmark)

    Christensen, K; Chwalibog, André; Henckel, S;

    1988-01-01

    the difference. 6. In pigs receiving a cereal based diet, HE(RQ) may be expected to give 3-4% higher values than HE(CN), but in case easily available carbohydrates (glucose, sucrose) or high-fibre diets are provided, the differences may be larger. 7. Both methods were carried out with similar accuracy......-balances in general were overestimated due to losses in excreta. 5. The difference between HE(RQ) and HE(CN) was not constant. It was smallest when fat synthesis and fat deposition were low (2-3.5%) and greatest when fat synthesis and fat deposition were high (7-9%). The presence of dietary fatty acids may affect...

  13. Effects of glutamate on distortion-product otoacoustic emissions and auditory brainstem responses in guinea pigs

    Institute of Scientific and Technical Information of China (English)

    SUN Qing; SUN Jian-he; SHAN Xi-zheng; LI Xing-qi

    2008-01-01

    Objective To investigate changes in evoked potentials and structure of the guinea pig cochleae during whole cochlear perfusion with glutamate. Methods CM, CAP, DPOAE, and ABR were recorded as indicators of cochlear functions during whole cochlear perfusion. The morphology of the cochlea was studied via transmission electron microscopy. Results There were no significant changes in DPOAE amplitude before and after glutamate perfusion. CM I/O function remained nonlinear during perfusion. ABR latencies were delayed following glutamate perfusion. The average CAP threshold was elevated 35 dB SPL following glutamate perfusion.. The OHCs appeared normal, but the IHCs and afferent dendrites showed cytoplasmic blebs after glutamate perfusion. Conclusions While being a primary amino acid neurotransmitter at the synapses between hair cells and spiral ganglion neurons, excessive glutamate is neurotoxic and can destroy IHCs and spiral ganglion neurons. The technique used in this study can also be used to build an animal model of auditory neuropathy.

  14. Impacts of amino acid nutrition on pregnancy outcome in pigs: mechanisms and implications for swine production.

    Science.gov (United States)

    Wu, G; Bazer, F W; Burghardt, R C; Johnson, G A; Kim, S W; Li, X L; Satterfield, M C; Spencer, T E

    2010-04-01

    Pigs suffer up to 50% embryonic and fetal loss during gestation and exhibit the most severe naturally occurring intrauterine growth retardation among livestock species. Placental insufficiency is a major factor contributing to suboptimal reproductive performance and reduced birth weights of pigs. Enhancement of placental growth and function through nutritional management offers an effective solution to improving embryonic and fetal survival and growth. We discovered an unusual abundance of the arginine family of AA in porcine allantoic fluid (a reservoir of nutrients) during early gestation, when placental growth is most rapid. Arginine is metabolized to ornithine, proline, and nitric oxide, and these compounds possess a plethora of physiological functions. Nitric oxide is a vasodilator and angiogenic factor, whereas both ornithine and proline are substrates for placental synthesis of polyamines, which are key regulators of protein synthesis and angiogenesis. Additionally, arginine, leucine, glutamine, and proline activate the mammalian target of rapamycin cell-signaling pathway to enhance protein synthesis and cell proliferation in placentae. To translate basic research on AA biochemistry and nutrition into application, dietary supplementation with 0.83% l-arginine to gilts on d 14 to 28 or d 30 to 114 of gestation increased the number and litter birth weight of live-born piglets. In addition, supplementing the gestation diet with 0.4% l-arginine plus 0.6% l-glutamine enhanced the efficiency of nutrient utilization, reduced variation in piglet birth weight, and increased litter birth weight. By regulating syntheses of nitric oxide, polyamines, and proteins, functional AA stimulate placental growth and the transfer of nutrients from mother to embryo or fetus to promote conceptus survival, growth, and development.

  15. Sodium nitrite induces acute central nervous system toxicity in guinea pigs exposed to systemic cell-free hemoglobin

    Energy Technology Data Exchange (ETDEWEB)

    Buehler, Paul W.; Butt, Omer I. [Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD (United States); D' Agnillo, Felice, E-mail: felice.dagnillo@fda.hhs.gov [Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD (United States)

    2011-06-10

    Highlights: {yields} Toxicological implications associated with the use of NaNO{sub 2} therapy to treat systemic cell-free Hb exposure are not well-defined. {yields} Systemic Hb exposure followed by NaNO{sub 2} infusion induces acute CNS toxicities in guinea pigs. {yields} These CNS effects were not reproduced by the infusion of cell-free Hb or NaNO{sub 2} alone. {yields} NaNO{sub 2}-mediated oxidation of cell-free Hb may play a causative role in the observed CNS changes. -- Abstract: Systemic cell-free hemoglobin (Hb) released via hemolysis disrupts vascular homeostasis, in part, through the scavenging of nitric oxide (NO). Sodium nitrite (NaNO{sub 2}) therapy can attenuate the hypertensive effects of Hb. However, the chemical reactivity of NaNO{sub 2} with Hb may enhance heme- or iron-mediated toxicities. Here, we investigate the effect of NaNO{sub 2} on the central nervous system (CNS) in guinea pigs exposed to systemic cell-free Hb. Intravascular infusion of NaNO{sub 2}, at doses sufficient to alleviate Hb-mediated blood pressure changes, reduced the expression of occludin, but not zona occludens-1 (ZO-1) or claudin-5, in cerebral tight junctions 4 h after Hb infusion. This was accompanied by increased perivascular heme oxygenase-1 expression, neuronal iron deposition, increased astrocyte and microglial activation, and reduced expression of neuron-specific nuclear protein (NeuN). These CNS changes were not observed in animals treated with Hb or NaNO{sub 2} alone. Taken together, these findings suggest that the use of nitrite salts to treat systemic Hb exposure may promote acute CNS toxicity.

  16. Nonrenewable Energy Cost and Greenhouse Gas Emissions of a “Pig-Biogas-Fish” System in China

    Directory of Open Access Journals (Sweden)

    Qing Yang

    2012-01-01

    Full Text Available The purpose of this study is to assess the energy savings and emission reductions of the present rural biogas system in China. The life cycle assessment (LCA method is used to analyze a “pig-biogas-fish” system in Jingzhou, Hubei Province, China. The nonrenewable energy cost and the greenhouse gas (GHG emissions of the system, including the pigsty, the biogas digester, and the fishpond, are taken into account. The border definition is standardized because of the utilization of the database in this paper. The results indicate that the nonrenewable energy consumption intensity of the “pig-biogas-fish” system is 0.60 MJ/MJ and the equivalent CO2 emission intensity is 0.05 kg CO2-eq/MJ. Compared with the conventional animal husbandry system, the “pig-biogas-fish” system shows high renewability and GHG reduction benefit, which indicates that the system is a scientific and environmentally friendly chain combining energy and ecology.

  17. Methane content and potential of biogas' production by manure of pigs fed with corn or sorghum-based diets in different phases

    Energy Technology Data Exchange (ETDEWEB)

    Miranda, Adelia Pereira; Lucas Junior, Jorge de; Thomaz, Maria Cristina; Fukayama, Ellen Hatsumi [Universidade Estadual Paulista (UNESP), Jaboticabal, SP (Brazil)

    2008-07-01

    The aim of this trial was to value the methane content and potential of biogas' production by manure of pigs fed with corn or sorghum-based diets (energy's source) in different phases. The manures were daily collected, diluted in water and homogeneous. Were used 267 mL of influent with 6% total solids concentration for supply of continuous-flow laboratory anaerobic digesters, with 30 days of detention time (DT) and temperature of 35 deg C. The experiment was completely randomized design with 2 treatments (corn or sorghum) and 3 phases of growth (initial x growing x finishing) in 10 replications (anaerobic digesters). The methane contents by manure of pigs fed with corn were greater (p<0.01) than pigs fed with sorghum (65.19 and 67.32%) on the phases of growing and finishing, respectively. The most potential of biogas' production (0.181, 0.138 and 0.162 m{sup 3}/kg of manure) was with manure of pigs fed with corn on the phases of initial, growing and finishing, respectively (p<0.01). Considering all the phases, the most potential of biogas' production (p<0.01) was observed in the initial phase, in both treatments. (author)

  18. Alternative farrowing accommodation: welfare and economic aspects of existing farrowing and lactation systems for pigs.

    Science.gov (United States)

    Baxter, E M; Lawrence, A B; Edwards, S A

    2012-01-01

    There is growing societal pressure, expressed through government legislation and consumers' purchasing choices, to abolish livestock systems considered detrimental to farm animal welfare. Such systems include farrowing crates, which are behaviourally and physically restrictive for sows. Therefore, identifying less restrictive farrowing systems for commercial implementation has become an important focus of pig research. Despite numerous attempts to develop indoor alternatives to crates, there is as yet no universal acceptance of such systems at the commercial level. The primary concern is piglet survival, because often favourable figures are reported at the experimental level, but not replicated in commercial evaluation. Alternative farrowing systems should equal or surpass survival levels in conventional systems and perform consistently across a range of farm circumstances for widespread commercial implementation. In addition, it is important that alternatives consider ease of management, operator safety and economic sustainability. Utilising a large database of literature, 12 existing alternative indoor systems were identified and compared against each other, conventional crates and outdoor systems. An assessment of how well alternative systems satisfy the design criteria for meeting animals' biological needs was carried out by developing a welfare design index (WDI). The physical and financial performance of these systems was also evaluated and summarised. The derived WDI yielded values of 0.95 for conventional crates, with higher scores for commercial outdoor systems of 1.10 and indoor group farrowing or multi-suckling systems (e.g. Thorstensson = 2.20). However, the high total piglet mortality (23.7% ± s.e. 2.26) in indoor group systems compared with conventional crates (18.3% ± s.e. 0.63) and outdoor systems (17.0% ± s.e. 2.05), together with the added capital cost (92% more than conventional crates, 249% more than commercial outdoor huts), mainly as a

  19. Performance of the macrophyte Lemna valdiviana in tertiary pig waste treatment and its contribution to the sustainability of swine production

    Directory of Open Access Journals (Sweden)

    Flávio R. Lapolli

    2008-03-01

    Full Text Available The present study aims to contribute to the sustainability of swine production by evaluating usage of the aquatic macrophyte Lemna valdiviana in the tertiary treatment of pig waste. Five assays (1 to 5 in triplicate were conducted using swine effluent with different COD (Chemical Oxygen Demand concentrations: 400, 550, 700, 850 and 1,000mg.L-1, respectively. The trial lasted for 21 days and the evaluated variables were: (a pollutant removal efficiency, (b biomass production and (c plant protein content under the different detention times of 7, 14 and 21 days. In general, assays 1 and 2 (CODs of 400 and 550mg.L-1 presented the best removal efficiencies under a detention time of 21 days. Regarding the purpose of both nutrient removal and production of high protein biomass, assay 3 (COD of 700mg.L-1 showed the best results under 14 days’ detention time (36.81% crude protein. It was established that the use of duckweeds in the tertiary swine waste treatment was able to provide a sustainable alternative regarding its advantages such as effluent polishing, minimization of environmental impact, and production of high protein feed.

  20. Geographic information systems: a useful tool to approach African swine fever surveillance management of wild pig populations.

    Science.gov (United States)

    Rolesu, Sandro; Aloi, Daniela; Ghironi, Annalisa; Oggiano, Nicolino; Oggiano, Annalisa; Puggioni, Giantonella; Patta, Cristiana; Farina, Salvatore; Montinaro, Salvatore

    2007-01-01

    The epidemiological surveillance of African swine fever in wild pig populations requires the previous collection of numerous samples of biological materials for virological and serological testing from each animal that has been killed during the hunting season. The number of samples needs to demonstrate the absence of the disease at a prevalence level of 5% (and confidence level of 95%) in the area subject observed. Since the typology of the territory suitable for maintaining wild pig populations and the precise location can be identified, it is possible to pinpoint specific areas within Sardinia where organised sampling is undertaken. The results from tests are used to estimate the prevalence of the disease in the wild pig population in the place of origin. Areas were identified using the geographic information system technology with support from maps in the field. The correct localisation of seropositivity has led to the redefinition of high-risk areas for African swine fever. Results from the outbreaks and the surveillance of the wild pig population has confirmed the decreasing role of the wild boar in maintaining the disease.

  1. Evaluation of the relationship between the biosecurity status, production parameters, herd characteristics and antimicrobial usage in farrow-to-finish pig production in four EU countries.

    Science.gov (United States)

    Postma, Merel; Backhans, Annette; Collineau, Lucie; Loesken, Svenja; Sjölund, Marie; Belloc, Catherine; Emanuelson, Ulf; Grosse Beilage, Elisabeth; Nielsen, Elisabeth Okholm; Stärk, Katharina D C; Dewulf, Jeroen

    2016-01-01

    High antimicrobial usage and the threat of antimicrobial resistance highlighted the need for reduced antimicrobial usage in pig production. Prevention of disease however, is necessary to obtain a reduced need for antimicrobial treatment. This study aimed at assessing possible associations between the biosecurity level, antimicrobial usage and farm and production characteristics in order to advice on best practices for a low antimicrobial usage and maximum animal health and production. A cross-sectional study was conducted in 227 farrow-to-finish pig herds in Belgium, France, Germany and Sweden between December 2012 and December 2013. Associations between biosecurity status, antimicrobial usage, and production parameters were evaluated with multivariable general linear models, according to an assumed causal pathway. The results showed that higher antimicrobial usage in sows tended to be associated with higher antimicrobial usage from birth until slaughter (p = 0.06). The antimicrobial usage from birth until slaughter was positively associated with the number of pathogens vaccinated against (p < 0.01). A shorter farrowing rhythm (p < 0.01) and a younger weaning age (p = 0.06) tended to be also associated with a higher antimicrobial usage from birth until slaughter whereas a better external biosecurity (p < 0.01) was related with a lower antimicrobial usage from birth until slaughter. Management practices such as weaning age and biosecurity measures may be important factors indirectly impacting on antimicrobial usage. We therefore promote a holistic approach when assessing the potential to reduce the need for antimicrobial treatments.

  2. Relationships between the H and A-O blood types, phosphohexose isomerase and 6-phosphogluconate dehydrogenase red cell enzyme systems and halothane sensitivity, and economic traits in a superior and an inferior selection line of swiss landrace pigs.

    Science.gov (United States)

    Vögeli, P; Stranzinger, G; Schneebeli, H; Hagger, C; Künzi, N; Gerwig, C

    1984-12-01

    Associations between production traits and the genes for halothane sensitivity (HAL), S, A and H blood group systems and phosphohexose isomerase (PHI) and 6-phosphogluconate dehydrogenase (6-PGD) enzyme systems were investigated in two lines of pigs selected for an index. The phenotypic variance-covariance matrix of the index included backfat thickness and daily gain, whereas the genetic variance-covariance matrix included daily gain, feed conversion and percentage of lean meat. The experiment was conducted at the experimental station of the Institute of Animal Production and has been underway since 1973. The same index was applied but in two opposite directions to give a superior and inferior line in relation to the production traits. One hundred twenty-nine animals of the superior line in the seventh generation and 88 animals of the inferior line in the sixth generation were studied. Forty-two percent (54/129) of the animals of the superior line were halothane-positive. No animals in the inferior line were halothane reactors. Of the halothane-positive pigs, 70.4% (38/54) in the superior line had the HaHa and 94.4% (51/54) had the SsSs genotype, whereas only 4% (3/75) of the HaHa and 12% (9/75) of the SsSs pigs were halothane-negative. By practicing selection at the H and S loci, it seems possible to efficiently reduce halothane sensitivity in Swiss Landrace pigs. In pigs of the superior line, there were significant differences in percentage of lean meat, carcass length, pH1 (pH value at 45 min to 1 h postmortem, M. longissimus) and reflectance values among genotypes of the HAL, S and H systems and among some genotypes of the 6-PGD system. Poorest meat quality, highest percentage of lean meat and shortest carcass length were observed in pigs homozygous for the alleles HALn, Ss, Ha, PHIB and 6-PGDA. In the inferior line, these associations were absent. As the HAL locus is associated with the above mentioned production traits, linkage disequilibria may explain the

  3. Energy use in pig farming

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-02-15

    This guide presents benchmark data on 'typical' and 'good practice' levels of energy consumption for pig farms in the UK. Benchmarks are valuable because they allow producers to compare their performance with other similar businesses. In addition, they also provide two other useful functions: They allow routine assessments to be made that show progress against a benchmark. Such appraisals are not restricted to year-on-year evaluations as they can be carried out quarterly, monthly or even weekly to track progress on a more frequent basis Opportunity assessments can be carried out. For example, if a facility is to be modified or upgraded, the effect of the change can be determined. Throughout this guide, the benchmarks and information are based on methods and techniques that minimise energy consumption whilst maintaining pig performance at an economically acceptable level. Pig farming in the UK is a complex and diverse business with a variety of facilities being used for each stage of production. Therefore, in order to give realistic guidelines, production has been broken down into several key areas to illustrate typical performance benchmarks. These production areas are: farrowing accommodation; weaning accommodation; finisher accommodation; feeding system; and waste handling. The guide has a detailed breakdown with graphs and data analysis.

  4. Optical studies of nicotinic acetylcholine receptor subtypes in the guinea-pig enteric nervous system.

    Science.gov (United States)

    Obaid, A L; Nelson, M E; Lindstrom, J; Salzberg, B M

    2005-08-01

    Nicotinic transmission in the enteric nervous system (ENS) is extensive, but the role of individual nicotinic acetylcholine receptor (nAChR) subtypes in the functional connectivity of its plexuses has been elusive. Using monoclonal antibodies (mAbs) against neuronal alpha3-, alpha4-, alpha3/alpha5-, beta2-, beta4- and alpha7-subunits, combined with radioimmunoassays and immunocytochemistry, we demonstrate that guinea-pig enteric ganglia contain all of these nAChR-subunits with the exception of alpha4, and so, differ from mammalian brain. This information alone, however, is insufficient to establish the functional role of the identified nAChR-subtypes within the enteric networks and, ultimately, their specific contributions to gastrointestinal physiology. We have used voltage-sensitive dyes and a high-speed CCD camera, in conjunction with specific antagonists to various nAChRs, to elucidate some of the distinct contributions of the individual subtypes to the behaviour of enteric networks. In the guinea-pig, the submucous plexus has the extraordinary advantage that it is virtually two-dimensional, permitting optical recording, with single cell resolution, of the electrical activity of all of its neurones. In this plexus, the block of alpha3beta2-, alpha3beta4- and/or alpha7-nAChRs always results in a decrease in the magnitude of the synaptic response. However, the magnitude of the fast excitatory post-synaptic potentials (epsps) evoked by electrical stimulation of a neighbouring ganglion varies from cell to cell, reflecting the differential expression of subunits already observed using mAbs, as well as the strengths of the activated synaptic inputs. At the same time, we observe that submucous neurones have a substantial mecamylamine (Mec)-insensitive (non-nicotinic) component to their fast epsps, which may point to the presence of purinergic or serotonergic fast epsps in this system. In the myenteric plexus, on the other hand, the antagonist-induced changes in the

  5. Comparative performance and microbial community of single-phase and two-phase anaerobic systems co-digesting cassava pulp and pig manure

    DEFF Research Database (Denmark)

    Panichnumsin, P.; Ahring, B.K.; Nopharatana, A.

    2010-01-01

    In this study, we illustrated the performance and microbial community of single- and two-phase systems anaerobically co-digesting cassava pulp and pig manure. The results showed that the volatile solid reduction and biogas productivity of two-phase CSTR were 66 ± 4% and 2000 ± 210 ml l-1 d-1, while...... predominant in both digesters while the relative abundance of Methanosaeta sp. and Methanospirillum hungatei differed between the two systems......., followed by the Clostridia in singlephase CSTR. In hydrolysis/acidification reactor of two-phase system, the bacteria within the phylum Firmicutes, especially Clostridium, Eubacteriaceae and Lactobacillus were the dominant phylogenetic groups. Among the Archaea, Methanosaeta sp. was the exclusive...

  6. Utilization of wasted sardine oil as co-substrate with pig slurry for biogas production--a pilot experience of decentralized industrial organic waste management in a Portuguese pig farm.

    Science.gov (United States)

    Ferreira, L; Duarte, E; Figueiredo, D

    2012-07-01

    This work aimed to demonstrate in a pig farm and in real conditions, the possibilities to co-digest wasted sardine oil (WSO) and pig slurry (PS) at farm scale. A biogas mobile pilot plant, was set up in the farm and operated in real conditions during 4 months. Dynamic mesophilic (35-37 °C) continuous pilot trials were performed during four different periods of time. In each period a different organic loading rate (OLR) based on the chemical oxygen demand (COD) was operated sequentially, with pig slurry (PS) (OLR = 1.6 kg COD/m(3) d(-1)) and with mixtures of WSO:PS with a volumetric composition (% v/v) of 2:98 (OLR = 3.0 kg COD/m(3) d(-1)), 3:97 (OLR = 3.7 kg COD/m(3) d(-1)) and 5:95 (OLR = 5.2 kg COD/m(3) d(-1)). Biomass adapted very fast in metabolise the WSO and biogas productivity was raised substantially for different compositions of WSO:PS. Process stability indicators pH and Total volatile fatty acids/bicarbonate alkalinity (T-VFA/BA) ratio, suggests that the co-digestion process was robust. It was concluded that WSO could be easily co-digested in farm scale biogas plants. Copyright © 2012. Published by Elsevier Ltd.

  7. Emerging Trichinella britovi infections in free ranging pigs of Greece.

    Science.gov (United States)

    Boutsini, S; Papatsiros, V G; Stougiou, D; Marucci, G; Liandris, E; Athanasiou, L V; Papadoudis, A; Karagiozopoulos, E; Bisias, A; Pozio, E

    2014-01-31

    Trichinella infections in humans and pigs have been documented in Greece since 1945 and a high prevalence of infection in pigs occurred in the 1950s. Up to 1984 only sporadic infections in humans were documented, and this zoonosis was not considered as a public health problem until 2009 when a human outbreak caused by the consumption of pork from an organic pig farm occurred. In the present study, we describe the re-emergence of Trichinella spp. infections in free-ranging pigs from organic farms of 3 counties (Dramas, Evros and Kavala) in Northern-Eastern Greece during the period 2009-2012. Totally 37 out of 12,717 (0.29%) free-ranging pigs which were tested during the period in question, were positive for Trichinella spp. larvae. The etiological agent was identified as Trichinella britovi. The average larval burden was 13.7 in the masseter, 6.2 in the foreleg muscles and 7.5 in the diaphragm. The 37 positive animals originated from seven free range pig farms. The practice of organic pig production systems in Greece has grown in popularity over the last years due to the increasing interest of consumers for products considered as traditional. However, this type of pig production increases the risk for Trichinella spp. infections, since animals can acquire the infection by feeding on carcasses or the offal of hunted or dead wild animals. The awareness and education of hunters and farmers is extremely important to reduce the transmission among free ranging pigs and the risk for humans.

  8. The system around the product

    DEFF Research Database (Denmark)

    Morelli, Nicola

    2006-01-01

    The design discipline has traditionally focused on products’ physical aspects, giving for granted that the context of services, infrastructures and technology will support the product life. A systemic perspective would revise such a focus, in order to project design competences over systemic...... and methods to handle immaterial aspects such as time sequences, actors’ role and logical links in a product service system (PSS). Tools and methods can be borrowed from other disciplinary contexts, their relevance, though, derives from their adaptation to this specific study area: designing in a systemic...... context. This paper describes a curricular activity focused on the definition of methodologies for designing in a systemic context. using examples from students’ projects. For a semester students had to focus on a project of a PSS, borrowing and re-adapting methods from other disciplines with the purpose...

  9. Inner ear drug delivery via a reciprocating perfusion system in the guinea pig.

    Science.gov (United States)

    Chen, Zhiqiang; Kujawa, Sharon G; McKenna, Michael J; Fiering, Jason O; Mescher, Mark J; Borenstein, Jeffrey T; Swan, Erin E Leary; Sewell, William F

    2005-12-10

    Rapid progress in understanding the molecular mechanisms associated with cochlear and auditory nerve degenerative processes offers hope for the development of gene-transfer and molecular approaches to treat these diseases in patients. For therapies based on these discoveries to become clinically useful, it will be necessary to develop safe and reliable mechanisms for the delivery of drugs into the inner ear, bypassing the blood-labyrinthine barrier. Toward the goal of developing an inner ear perfusion device for human use, a reciprocating microfluidic system that allows perfusion of drugs into the cochlear perilymph through a single inlet hole in scala tympani of the basal turn was developed. The performance of a prototype, extracorporeal reciprocating perfusion system in guinea pigs is described. Analysis of the cochlear distribution of compounds after perfusion took advantage of the place-dependent generation of responses to tones along the length of the cochlea. Perfusion with a control artificial perilymph solution had no effect. Two drugs with well-characterized effects on cochlear physiology, salicylate (5 mM) and DNQX (6,7-Dinitroquinoxaline-2,3-dione; 100 and 300 microM), reversibly altered responses. The magnitude of drug effect decreased with distance from the perfusion pipette for up to 10 mm, and increased with dose and length of application.

  10. Performance and energy metabolism in restrictively fed weanling pigs are not affected by feeding either fermented cereals or their end-products.

    Science.gov (United States)

    Bruininx, E M A M; Binnendijk, G P; Zandstra, T; Heetkamp, M J W; Van Der Peet-Schwering, C M C; Gerrits, W J J

    2010-12-01

    To study the effects of feeding fermented cereals or just fermentation end-products on performance and energy metabolism, 18 restrictedly fed groups of eight pigs each were assigned to one of three dietary treatments: (i) a liquid control diet (C) containing 40% of a mixture of barley and wheat; or (ii) a liquid diet (F) containing 40% fermented barley and wheat; or (iii) a liquid diet as C with the addition of some important fermentation end-products (FP; organic acids and ethanol) in concentrations similar to those in the fermented F-diet. Energy and nitrogen balances, heat production, and performance traits were measured during two consecutive periods (days 1-5 and days 6-14). There was a considerable increase in average dry matter intake that tended (p = 0.06) to be higher in the FP-group than in the other groups. Apparent fecal digestibility of dry matter, ash, nitrogen and energy during period 2 were not affected (p > 0.1). Averaged over both periods, none of the energy metabolism parameters were affected by the diets (p > 0.1). However, there were diet × period interactions for metabolizable energy-intake (p = 0.07), energy retention (p feeding of either 40% fermented cereals nor their fermentation end-products affected performance and energy metabolism traits in weanling pigs. Nevertheless, lower postprandial activity-related heat production by pigs given the fermented cereals suggest a stimulating effect of fermented cereals on short term satiety that was not seen in pigs given fermentation end-products only.

  11. The ATLAS Production System Evolution

    CERN Document Server

    Borodin, Mikhail; The ATLAS collaboration

    2017-01-01

    The second generation of the ATLAS Production System called ProdSys2 is a distributed workload manager that runs daily hundreds of thousands of jobs, from dozens of different ATLAS-specific workflows, across more than a hundred heterogeneous sites. It achieves high utilization by combining dynamic job definition based upon many criteria, such as input and output size, memory requirements and CPU consumption, with manageable scheduling policies and by supporting different kinds of computational resources, such as GRID, clouds, supercomputers and volunteer computers. The system dynamically assigns a group of jobs (task) to a group of geographically distributed computing resources. Dynamic assignment and resource utilization is one of the major features of the system. The Production System has a sophisticated job fault recovery mechanism, which efficiently allows running multi-terabyte tasks without human intervention. We have implemented new features which allow automatic task submission and chaining of differe...

  12. Study of Ammonia Emissions in a Ventilated Pig Pen

    DEFF Research Database (Denmark)

    Rong, Li

    Pig productions cause a wide emission of odors, such as ammonia (NH3), hydrogen sulfide (H2S), and methane (CH4). Ammonia is one of the most important emissions for evaluating the air quality either in animal buildings or atmospheric environment. In studies of ammonia emission from animal buildings...... solution temperatures. The results show that the diffusive ceiling ventilation system can provide a relative low velocity in the pig pen and decrease ammonia emissions from the pig pen, but this ventilation system causes high ammonia concentration distribution in the animal occupied zone. Further, our...... reported in literature, little effort has been made to investigate the accuracy of current Henry’s law constant for modeling ammonia mass transfer process and study ammonia emissions in a full scale pig pen from fluid dynamics by CFD simulations. This will be the main objectives of this study. The ammonia...

  13. Biogas production and microbial community shift through neutral pH control during the anaerobic digestion of pig manure.

    Science.gov (United States)

    Zhou, Jun; Zhang, Rui; Liu, Fenwu; Yong, Xiaoyu; Wu, Xiayuan; Zheng, Tao; Jiang, Min; Jia, Honghua

    2016-10-01

    Laboratory-scale reactors, in which the pH could be auto-adjusted, were employed to investigate the mesophilic methane fermentation with pig manure (7.8% total solids) at pH 6.0, 7.0, and 8.0. Results showed that the performance of anaerobic digestion was strongly dependent on pH value. Biogas production and methane content at neutral pH 7.0 were significantly higher (16,607mL, 51.81%) than those at pH 6.0 (6916mL, 42.9%) and 8.0 (9739mL, 35.6%). Denaturing gradient gel electrophoresis fingerprinting and Shannon's index indicated that the samples contained highly diverse microbial communities. The major genus at pH 7.0 was Methanocorpusculum, compared with that was Methanosarcina at both pH 6.0 and 8.0. Our research revealed that cultures maintained at pH 7.0 could support increased biogas production, which has significant implications for the scale-up biogas engineering.

  14. Genotype by environment interaction for carcass traits and intramuscular fat content in heavy Iberian pigs fattened in two different free-range systems

    Directory of Open Access Journals (Sweden)

    Juan M. García Casco

    2014-04-01

    Full Text Available Genotype by environment interaction (G×E is a potential source of reduced efficiency in genetic improvement programs in livestock. The objective of the current work consisted of checking the existence of G×E interaction in carcass traits and in intramuscular fat content (IMF in Iberian pigs fattened in two free-range systems. Genetic component and estimated breeding values (EBV of the percentage of hams, shoulders and loins and IMF in loin were obtained from records of 4,348 and 1,818 pigs fattened in campo (C and montanera (M systems, respectively. A multitrait model where the performances of each system are considered as different traits was implemented. Three selection indexes were built with different treatments about the quality trait, two of them based in the optimal trait theory. The Pearson correlation between EBV and indexes and the Spearman correlation between the rankings of progenies of 21 boars fattened in both systems were calculated. Heritability results were different in both systems (h2 range from 0.43 to 0.66 and from 0.24 to 0.33 in C and M system, respectively and genetic correlation of same traits expressed in the two systems also pointed out to a weak G×E interaction (0.64, 0.67 and 0.66 in hams, shoulders and IMF, respectively. Pearson and Spearman correlations were always significantly different to 1. The obtained results advised to consider this G×E interaction in the analysis model of a breeding program focused on free range production system and to include IMF in the index selection assuming an optimum range for this quality trait, in order to avoid negative effects of selection for carcass performances.

  15. An assessment of soybeans and other vegetable proteins as source of salmonella contamination in pig production

    Directory of Open Access Journals (Sweden)

    Häggblom Per

    2010-02-01

    Full Text Available Abstract Background The impact of salmonella contaminated feed ingredients on the risk for spreading salmonella to pigs was assessed in response to two incidences when salmonella was spread by feed from two feed mills to 78 swine producing herds. Methods The assessment was based on results from the salmonella surveillance of feed ingredients before introduction to feed mills and from HACCP - based surveillance of the feed mills. Results from the mills of the Company (A that produced the salmonella contaminated feed, were by the Chi. Square test compared to the results from all the other (B - E feed producers registered in Sweden. Isolated serovars were compared to serovars from human cases of salmonellosis. Results Salmonella (28 serovars was frequently isolated from imported consignments of soybean meal (14.6% and rape seed meal (10.0%. Company A largely imported soybean meal from crushing plants with a history of unknown or frequent salmonella contamination. The risk for consignments of vegetable proteins to be salmonella contaminated was 2.4 times (P Conclusions Salmonella contaminated feed ingredients are an important source for introducing salmonella into the feed and food chain. Effective HACCP-based control and associated corrective actions are required to prevent salmonella contamination of feed. Efforts should be taken to prevent salmonella contamination already at the crushing plants. This is challenge for the EU - feed industry due to the fact that 98% of the use of soybean/meal, an essential feed ingredient, is imported from crushing plants of third countries usually with an unknown salmonella status.

  16. Characterization of microbial communities in exhaust air treatment systems of large-scale pig housing facilities.

    Science.gov (United States)

    Haneke, J; Lee, N M; Gaul, T W; Van den Weghe, H F A

    2010-01-01

    Exhaust air treatment has gained importance as an essential factor in intensive livestock areas due to the rising emissions in the environment. Wet filter walls of multi-stage exhaust air treatment systems precipitate gaseous ammonia and dust particles from exhaust air in washing water. Microbial communities in the biomass developed in the washing water of five large-scale exhaust air treatment units of pig housing facilities, were investigated by fluorescence in situ hybridization (FISH) and 16S rDNA sequence analyses. No "standard" nitrifying bacteria were found in the washing water. Instead mainly α-Proteobacteria, aggregating β- and χ-Proteobacteria, a large number of Actinobacteria, as well as individual Planctomycetales and Crenarchaeota were detected after more than twelve months' operation. The main Proteobacteria species present were affiliated to the families Alcaligenaceae, Comamonadaceae and Xanthomonadaceae. Furthermore, we investigated the consumption of inorganic nitrogen compounds in the washing water of one exhaust air treatment unit during a fattening period with and without pH control. Maintaining the pH at 6.0 resulted in a ca. fivefold higher ammonium concentration and a ca. fourfold lower concentration of oxidized nitrogen compounds after the fattening period was finished.

  17. Quantitative approach for the risk assessment of African swine fever and Classical swine fever introduction into the United States through legal imports of pigs and swine products

    Science.gov (United States)

    Herrera-Ibatá, Diana María; Martínez-López, Beatriz; Quijada, Darla; Burton, Kenneth

    2017-01-01

    The US livestock safety strongly depends on its capacity to prevent the introduction of Transboundary Animal Diseases (TADs). Therefore, accurate and updated information on the location and origin of those potential TADs risks is essential, so preventive measures as market restrictions can be put on place. The objective of the present study was to evaluate the current risk of African swine fever (ASF) and Classical swine fever (CSF) introduction into the US through the legal importations of live pigs and swine products using a quantitative approach that could be later applied to other risks. Four quantitative stochastic risk assessment models were developed to estimate the monthly probabilities of ASF and CSF release into the US, and the exposure of susceptible populations (domestic and feral swine) to these introductions at state level. The results suggest a low annual probability of either ASF or CSF introduction into the US, by any of the analyzed pathways (5.5*10−3). Being the probability of introduction through legal imports of live pigs (1.8*10−3 for ASF, and 2.5*10−3 for CSF) higher than the risk of legally imported swine products (8.90*10−4 for ASF, and 1.56*10−3 for CSF). This could be caused due to the low probability of exposure associated with this type of commodity (products). The risk of feral pigs accessing to swine products discarded in landfills was slightly higher than the potential exposure of domestic pigs through swill feeding. The identification of the months at highest risk, the origin of the higher risk imports, and the location of the US states most vulnerable to those introductions (Iowa, Minnesota and Wisconsin for live swine and California, Florida and Texas for swine products), is valuable information that would help to design prevention, risk-mitigation and early-detection strategies that would help to minimize the catastrophic consequences of potential ASF/CSF introductions into the US. PMID:28797058

  18. The system around the product

    DEFF Research Database (Denmark)

    Morelli, Nicola

    2006-01-01

    context. This paper describes a curricular activity focused on the definition of methodologies for designing in a systemic context. using examples from students’ projects. For a semester students had to focus on a project of a PSS, borrowing and re-adapting methods from other disciplines with the purpose......The design discipline has traditionally focused on products’ physical aspects, giving for granted that the context of services, infrastructures and technology will support the product life. A systemic perspective would revise such a focus, in order to project design competences over systemic...

  19. Compartmentalisation in aquaculture production systems.

    Science.gov (United States)

    Zepeda, C; Jones, J B; Zagmutt, F J

    2008-04-01

    Compartmentalisation is a new tool for disease management within a country. In aquaculture, the successful application of compartmentalisation is largely dependent on the system of production and the epidemiology of the disease(s) for which the compartment is being defined. Therefore, compartmentalisation may not be universally applicable across all systems and diseases. The paper examines the implementation of the concept, providing examples of specific industries, and discusses the application of hazard analysis and critical control points (HACCP) to the biosecurity of the system. The role of compartmentalisation in the management of aquaculture disease emergencies is also discussed.

  20. Nutritional value of high fiber co-products from the copra, palm kernel, and rice industries in diets fed to pigs.

    Science.gov (United States)

    Stein, Hans Henrik; Casas, Gloria Amparo; Abelilla, Jerubella Jerusalem; Liu, Yanhong; Sulabo, Rommel Casilda

    2015-01-01

    High fiber co-products from the copra and palm kernel industries are by-products of the production of coconut oil and palm kernel oil. The co-products include copra meal, copra expellers, palm kernel meal, and palm kernel expellers. All 4 ingredients are very high in fiber and the energy value is relatively low when fed to pigs. The protein concentration is between 14 and 22 % and the protein has a low biological value and a very high Arg:Lys ratio. Digestibility of most amino acids is less than in soybean meal but close to that in corn. However, the digestibility of Lys is sometimes low due to Maillard reactions that are initiated due to overheating during drying. Copra and palm kernel ingredients contain 0.5 to 0.6 % P. Most of the P in palm kernel meal and palm kernel expellers is bound to phytate, but in copra products less than one third of the P is bound to phytate. The digestibility of P is, therefore, greater in copra meal and copra expellers than in palm kernel ingredients. Inclusion of copra meal should be less than 15 % in diets fed to weanling pigs and less than 25 % in diets for growing-finishing pigs. Palm kernel meal may be included by 15 % in diets for weanling pigs and 25 % in diets for growing and finishing pigs. Rice bran contains the pericarp and aleurone layers of brown rice that is removed before polished rice is produced. Rice bran contains approximately 25 % neutral detergent fiber and 25 to 30 % starch. Rice bran has a greater concentration of P than most other plant ingredients, but 75 to 90 % of the P is bound in phytate. Inclusion of microbial phytase in the diets is, therefore, necessary if rice bran is used. Rice bran may contain 15 to 24 % fat, but it may also have been defatted in which case the fat concentration is less than 5 %. Concentrations of digestible energy (DE) and metabolizable energy (ME) are slightly less in full fat rice bran than in corn, but defatted rice bran contains less than 75 % of the DE and ME in

  1. The plasticity of the defecation reflex pathway in the enteric nervous system of guinea pigs.

    Science.gov (United States)

    Katsui, Renta; Kuniyasu, Hiroki; Matsuyoshi, Hiroko; Fujii, Hisao; Nakajima, Yoshiyuki; Takaki, Miyako

    2009-02-01

    The enteric nervous system, the "second" brain, is an independent nervous system that structurally resembles the "first" brain. Appropriate rectal distension elicits rectal (R-R) reflex contractions and simultaneous internal anal sphincter (R-IAS) reflex relaxations that together comprise the defecation reflex. The enteric nervous system, pelvic nerves and lumbar colonic nerves control both reflexes. Using the plasticity of enteric nervous pathways, a new therapy for repairing enteric neural dysfunction could be developed. In vivo experiments were performed on guinea pigs anesthetized with ethyl carbamate. We performed either a lower anterior resection as used for rectal cancer, without damaging the extrinsic nerves or a resection of a 2-cm segment of distal colon, 30 mm orally from the anal verge, with subsequent end-to-end one layer anastomosis of the exposed ends. The recovery of the defecation reflex was found to be the same in both the rectal transection and distal colonic resection procedures. Eight weeks after sectioning the intrinsic reflex nerve pathways in the rectum, the defecation reflex recovered to control levels, accompanied by a regeneration of the reflex pathways. The 5-HT(4) receptor agonist, mosapride (0.5 and 1.0 mg/kg), significantly (P<0.01) enhanced the recovered defecation reflex at this stage. Two weeks after local treatment with brain-derived neurotrophic factor (BDNF: 10(-6) g/ml) at the rectal anastomotic site, the R-IAS reflex relaxations recovered and some bundles of fine nerve fibers were able to be seen interconnecting the oral and anal ends of the myenteric plexus. Also surprisingly, new neurons were found to have generated from neural stem cells at the anastomotic ends. These new neurons had constructed mature enteric neural networks including ganglionic-like structures eight weeks after surgery. These results revealed the plasticity of enteric neurons, allowing the proposal of a new therapy for repairing enteric neural dysfunction

  2. Alert Systems for production Plants

    DEFF Research Database (Denmark)

    Nielsen, Thomas Dyhre; Jensen, Finn Verner

    2005-01-01

    We present a new methodology for detecting faults and abnormal behavior in production plants. The methodology stems from a joint project with a Danish energy consortium. During the course of the project we encountered several problems that we believe are common for projects of this type. Most...... system operation, i.e., it does not rely on information about the possible faults. We illustrate the proposed method using real-world data from a coal driven power plant as well as simulated data from an oil production facility....

  3. An Overview of Production Systems

    Science.gov (United States)

    1975-10-01

    ppear to be uawly recurrent thaws. This peper is e- stt4 to Provide 1an 0102124F21 -Ad Overview of those themes, as well as a c~ncept~Aal frmework...illustrate some of the foregoing notions. The production system, called PS.ONE, is assumed for thils example to contain two productions, PD I and PD2...having one section of code call another explicitly, but rather use an indirect approach in which each piece of code (i.e. each rule) leaves behind the

  4. Biogas Production from Protein-Rich Biomass: Fed-Batch Anaerobic Fermentation of Casein and of Pig Blood and Associated Changes in Microbial Community Composition

    Science.gov (United States)

    Kovács, Etelka; Wirth, Roland; Maróti, Gergely; Bagi, Zoltán; Rákhely, Gábor; Kovács, Kornél L.

    2013-01-01

    It is generally accepted as a fact in the biogas technology that protein-rich biomass substrates should be avoided due to inevitable process inhibition. Substrate compositions with a low C/N ratio are considered difficult to handle and may lead to process failure, though protein-rich industrial waste products have outstanding biogas generation potential. This common belief has been challenged by using protein-rich substrates, i.e. casein and precipitated pig blood protein in laboratory scale continuously stirred mesophilic fed-batch biogas fermenters. Both substrates proved suitable for sustained biogas production (0.447 L CH4/g protein oDM, i.e. organic total solids) in high yield without any additives, following a period of adaptation of the microbial community. The apparent key limiting factors in the anaerobic degradation of these proteinaceous materials were the accumulation of ammonia and hydrogen sulfide. Changes in time in the composition of the microbiological community were determined by next-generation sequencing-based metagenomic analyses. Characteristic rearrangements of the biogas-producing community upon protein feeding and specific differences due to the individual protein substrates were recognized. The results clearly demonstrate that sustained biogas production is readily achievable, provided the system is well-characterized, understood and controlled. Biogas yields (0.45 L CH4/g oDM) significantly exceeding those of the commonly used agricultural substrates (0.25-0.28 L CH4/g oDM) were routinely obtained. The results amply reveal that these high-energy-content waste products can be converted to biogas, a renewable energy carrier with flexible uses that can replace fossil natural gas in its applications. Process control, with appropriate acclimation of the microbial community to the unusual substrate, is necessary. Metagenomic analysis of the microbial community by next-generation sequencing allows a precise determination of the alterations in

  5. Biogas production from protein-rich biomass: fed-batch anaerobic fermentation of casein and of pig blood and associated changes in microbial community composition.

    Directory of Open Access Journals (Sweden)

    Etelka Kovács

    Full Text Available It is generally accepted as a fact in the biogas technology that protein-rich biomass substrates should be avoided due to inevitable process inhibition. Substrate compositions with a low C/N ratio are considered difficult to handle and may lead to process failure, though protein-rich industrial waste products have outstanding biogas generation potential. This common belief has been challenged by using protein-rich substrates, i.e. casein and precipitated pig blood protein in laboratory scale continuously stirred mesophilic fed-batch biogas fermenters. Both substrates proved suitable for sustained biogas production (0.447 L CH4/g protein oDM, i.e. organic total solids in high yield without any additives, following a period of adaptation of the microbial community. The apparent key limiting factors in the anaerobic degradation of these proteinaceous materials were the accumulation of ammonia and hydrogen sulfide. Changes in time in the composition of the microbiological community were determined by next-generation sequencing-based metagenomic analyses. Characteristic rearrangements of the biogas-producing community upon protein feeding and specific differences due to the individual protein substrates were recognized. The results clearly demonstrate that sustained biogas production is readily achievable, provided the system is well-characterized, understood and controlled. Biogas yields (0.45 L CH4/g oDM significantly exceeding those of the commonly used agricultural substrates (0.25-0.28 L CH4/g oDM were routinely obtained. The results amply reveal that these high-energy-content waste products can be converted to biogas, a renewable energy carrier with flexible uses that can replace fossil natural gas in its applications. Process control, with appropriate acclimation of the microbial community to the unusual substrate, is necessary. Metagenomic analysis of the microbial community by next-generation sequencing allows a precise determination of the

  6. Biogas production from protein-rich biomass: fed-batch anaerobic fermentation of casein and of pig blood and associated changes in microbial community composition.

    Science.gov (United States)

    Kovács, Etelka; Wirth, Roland; Maróti, Gergely; Bagi, Zoltán; Rákhely, Gábor; Kovács, Kornél L

    2013-01-01

    It is generally accepted as a fact in the biogas technology that protein-rich biomass substrates should be avoided due to inevitable process inhibition. Substrate compositions with a low C/N ratio are considered difficult to handle and may lead to process failure, though protein-rich industrial waste products have outstanding biogas generation potential. This common belief has been challenged by using protein-rich substrates, i.e. casein and precipitated pig blood protein in laboratory scale continuously stirred mesophilic fed-batch biogas fermenters. Both substrates proved suitable for sustained biogas production (0.447 L CH4/g protein oDM, i.e. organic total solids) in high yield without any additives, following a period of adaptation of the microbial community. The apparent key limiting factors in the anaerobic degradation of these proteinaceous materials were the accumulation of ammonia and hydrogen sulfide. Changes in time in the composition of the microbiological community were determined by next-generation sequencing-based metagenomic analyses. Characteristic rearrangements of the biogas-producing community upon protein feeding and specific differences due to the individual protein substrates were recognized. The results clearly demonstrate that sustained biogas production is readily achievable, provided the system is well-characterized, understood and controlled. Biogas yields (0.45 L CH4/g oDM) significantly exceeding those of the commonly used agricultural substrates (0.25-0.28 L CH4/g oDM) were routinely obtained. The results amply reveal that these high-energy-content waste products can be converted to biogas, a renewable energy carrier with flexible uses that can replace fossil natural gas in its applications. Process control, with appropriate acclimation of the microbial community to the unusual substrate, is necessary. Metagenomic analysis of the microbial community by next-generation sequencing allows a precise determination of the alterations in

  7. Production of methane by co-digestion of cassava pulp with various concentrations of pig manure

    DEFF Research Database (Denmark)

    Panichnumsin, Pan; Nopharatana, Annop; Ahring, Birgitte Kiær

    2010-01-01

    Cassava pulp is a major by-product produced in a cassava starch factory, containing 50-60% of starch (dry basis). Therefore, in this study we are considering its potential as a raw material substrate for the production of methane. To ensure sufficient amounts of nutrients for the anaerobic digest...

  8. Pig slurry characteristics, nutrient balance and biogas production as affected by separation and acidification

    DEFF Research Database (Denmark)

    Sommer, S G; Hjorth, Maibritt; Leahy, J J

    2015-01-01

    and separation fraction applied to fields and crop need. Total biogas production was not affected by separation, whereas acidification reduced biogas production because the process was inhibited by a low pH and a high sulphur concentration. The amount of copper applied per hectare in the liquid manure...

  9. EFFECT OF LIVER DENERVATION ON GLUCOSE-PRODUCTION DURING RUNNING IN GUINEA-PIGS

    NARCIS (Netherlands)

    WIERSMA, MML; VISSING, J; MIKINES, KJ; STEFFENS, AB; GALBO, H

    1995-01-01

    Activity in sympathetic liver nerves has been proposed to be important for glucose production in exercising humans. However, liver denervation does not influence the exercise-induced increase in glucose production in the rat and dog. These species have a poor sympathetic liver innervation in contras

  10. Weaning the pig - Concepts and consequences

    NARCIS (Netherlands)

    Pluske, J.R.; Dividich, Le J.; Verstegen, M.W.A.

    2003-01-01

    Weaning the Pig: Concepts and Consequences addresses the major issues surrounding the weaning process, both for piglets and the breeding herd, in modern-day pig production. The post-weaned pig presents many challenges to the manager, stockperson and nutritionist, and as such is a critical phase in t

  11. Weaning the pig - Concepts and consequences

    NARCIS (Netherlands)

    Pluske, J.R.; Dividich, Le J.; Verstegen, M.W.A.

    2003-01-01

    Weaning the Pig: Concepts and Consequences addresses the major issues surrounding the weaning process, both for piglets and the breeding herd, in modern-day pig production. The post-weaned pig presents many challenges to the manager, stockperson and nutritionist, and as such is a critical phase in t

  12. Weaning the pig - Concepts and consequences

    NARCIS (Netherlands)

    Pluske, J.R.; Dividich, Le J.; Verstegen, M.W.A.

    2003-01-01

    Weaning the Pig: Concepts and Consequences addresses the major issues surrounding the weaning process, both for piglets and the breeding herd, in modern-day pig production. The post-weaned pig presents many challenges to the manager, stockperson and nutritionist, and as such is a critical phase in

  13. Gaseous emissions, growth performance and pork quality of pigs housed in deep-litter system compared to concrete-floor system.

    Science.gov (United States)

    Zhou, Chuanshe; Hu, Jinjie; Zhang, Bin; Tan, Zhiliang

    2015-04-01

    This study measured gaseous emissions, growth performance and pork quality in a deep-litter system and concrete-floor system. Three hundred and twenty weaned piglets with an average body weight (BW) of 6.0 ± 0.3 kg were assigned randomly into three treatments. Treatments 1 and 2 included four pens with 20 pigs for each pen respectively in the deep-litter system, and the ratio of sawdust to chaff was 5:5 and 3:7 for treatments 1 and 2 respectively, the probiotics inoculated into the fermentation bedding for both treatments were composed of Saccharomycetes, Bacillus subtilis and Actinomycetes; treatment 3 was the conventional concrete-floor system including eight pens with 20 pigs for each pen. The concentration of NH3 and CO2 in the deep-litter system was significantly (P litter system was significantly (P litter system were significantly (P litter system had some animal welfare improvements and an odor nuisance reduction; in the meantime, pork quality also improved from the deep-litter system compared to the pigs housed in the concrete-floor system.

  14. Evaluation of sensitivity and specificity of routine meat inspection of Danish slaughter pigs using Latent Class Analysis

    DEFF Research Database (Denmark)

    Bonde, Marianne; Toft, Nils; Thomsen, Peter;

    2010-01-01

    Two groups of observers, regular meat inspectors and two veterinary researchers, respectively, conducted independent veterinary meat inspection of organs of slaughter pigs from organic or conventional production systems slaughtered at one abattoir in April 2005. A total of 3054 pigs (899 organic ...

  15. Productivity raising approaches in the logistic production system

    Directory of Open Access Journals (Sweden)

    Miroslav Rakyta

    2001-01-01

    Full Text Available This paper deals with the productivity of the logistic production system. The aim is to define the intermediate state of the production machines and equipment (OOE, identification of the material flows, idle time and breakdown forecasting, with the following purchase of the required spare parts. The goal of the system is the satisfaction of the customer´s needs and the minimalisation of not fulfilling the required terms for delivery with simultaneous increasing of the logistic production system productivity.

  16. Sodium nitrite induces acute central nervous system toxicity in guinea pigs exposed to systemic cell-free hemoglobin.

    Science.gov (United States)

    Buehler, Paul W; Butt, Omer I; D'Agnillo, Felice

    2011-06-10

    Systemic cell-free hemoglobin (Hb) released via hemolysis disrupts vascular homeostasis, in part, through the scavenging of nitric oxide (NO). Sodium nitrite (NaNO(2)) therapy can attenuate the hypertensive effects of Hb. However, the chemical reactivity of NaNO(2) with Hb may enhance heme- or iron-mediated toxicities. Here, we investigate the effect of NaNO(2) on the central nervous system (CNS) in guinea pigs exposed to systemic cell-free Hb. Intravascular infusion of NaNO(2), at doses sufficient to alleviate Hb-mediated blood pressure changes, reduced the expression of occludin, but not zona occludens-1 (ZO-1) or claudin-5, in cerebral tight junctions 4h after Hb infusion. This was accompanied by increased perivascular heme oxygenase-1 expression, neuronal iron deposition, increased astrocyte and microglial activation, and reduced expression of neuron-specific nuclear protein (NeuN). These CNS changes were not observed in animals treated with Hb or NaNO(2) alone. Taken together, these findings suggest that the use of nitrite salts to treat systemic Hb exposure may promote acute CNS toxicity.

  17. Expression of type 1 corticotropin-releasing factor receptor in the guinea pig enteric nervous system.

    Science.gov (United States)

    Liu, Sumei; Gao, Xiang; Gao, Na; Wang, Xiyu; Fang, Xiucai; Hu, Hong-Zhen; Wang, Guo-Du; Xia, Yun; Wood, Jackie D

    2005-01-17

    Reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemistry, electrophysiological recording, and intraneuronal injection of the neuronal tracer biocytin were integrated in a study of the functional expression of corticotropin-releasing factor (CRF) receptors in the guinea pig enteric nervous system. RT-PCR revealed expression of CRF1 receptor mRNA, but not CRF2, in both myenteric and submucosal plexuses. Immunoreactivity for the CRF1 receptor was distributed widely in the myenteric plexus of the stomach and small and large intestine and in the submucosal plexus of the small and large intestine. CRF1 receptor immunoreactivity was coexpressed with calbindin, choline acetyltransferase, and substance P in the myenteric plexus. In the submucosal plexus, CRF1 receptor immunoreactivity was found in neurons that expressed calbindin, substance P, choline acetyltransferase, or neuropeptide Y. Application of CRF evoked slowly activating depolarizing responses associated with elevated excitability in both myenteric and submucosal neurons. Histological analysis of biocytin-filled neurons revealed that both uniaxonal neurons with S-type electrophysiological behavior and neurons with AH-type electrophysiological behavior and Dogiel II morphology responded to CRF. The CRF-evoked depolarizing responses were suppressed by the CRF1/CRF2 receptor antagonist astressin and the selective CRF1 receptor antagonist NBI27914 and were unaffected by the selective CRF2 receptor antagonist antisauvagine-30. The findings support the hypothesis that the CRF1 receptor mediates the excitatory actions of CRF on neurons in the enteric nervous system. Actions on enteric neurons might underlie the neural mechanisms by which stress-related release of CRF in the periphery alters intestinal propulsive motor function, mucosal secretion, and barrier functions.

  18. Antibiotic resistance patterns of Escherichia coli strains isolated from surface water and groundwater samples in a pig production area

    Directory of Open Access Journals (Sweden)

    Roger Neto Schneider

    2009-09-01

    Full Text Available The use of antibiotics, so excessive and indiscriminate in intensive animal production, has triggered an increase in the number of resistant microorganisms which can be transported to aquatic environments. The aim of this study was to determine the profile of the antimicrobial resistance of samples of Escherichia coli isolated from groundwater and surface water in a region of pig breeding. Through the test of antimicrobial susceptibility, we analyzed 205 strains of E. coli. A high rate of resistance to cefaclor was observed, both in surface water (51.9% and groundwater (62.9%, while all samples were sensitive to amikacin. The percentages of multi-resistant samples were 25.96% and 26.73% in surface water and groundwater, respectively, while 19.23% and 13.86% were sensitive to all antibiotics tested. It was determined that the rate of multiple antibiotic resistance (MAR was 0.164 for surface water and 0.184 for groundwater. No significant differences were found in the profile of the antimicrobial resistance in strains of E. coli isolated in surface water and groundwater, but the index MAR calculated in certain points of groundwater may offer a potential risk of transmission of resistant genes.

  19. Assessment of welfare in pigs

    Directory of Open Access Journals (Sweden)

    Luisa Antonella Volpelli

    2010-01-01

    Full Text Available According to the definition given by Appleby (1996, animal welfare represents the state of well-being brought about by meeting the physical, environmental, nutritional, behavioural and social needs of the animal or groups of animals under the care, supervision or influence of people. Suitable husbandry techniques and disease control (in which man is directly involved may satisfy an animal’s physical, environmental and nutritive needs. However, it cannot be stated that people’s supervision or influence always guarantee the satisfaction of behavioural and social needs. Thus, special attention must be paid to these factors in intensive husbandry. This paper calls attention to the main factors characterizing pig welfare on the basis of productive, physiological, pathological and behavioural indicators; to the behavioural needs, which are characterised by several peculiar traits (it is noteworthy that, since the beginning, all categories of reared pigs have been involved in welfare legislation; to all categories of pigs that often show the effects of negative stimuli on their behaviour (limitations, variations; to the main critical points on the farm likely to cause welfare impairment or stress including buildings, inner facilities, space allowance, microclimate, lighting systems, environmental stressors, feeding management, mutilations, weaning, social factors, and stockmanship; and to environmental stressors including dust, odours (especially ammonia and noises. This paper takes into account sources, effects and possible solutions for noises; the positive effect of fibrous feeding; environmental enrichment and other possible techniques for improving social status and for preventing/reducing stereotypic behaviour and abnormal reactions (e.g. tail biting. The scientific/objective evaluation of welfare for intensively reared pigs may be carried out by means of direct observation of the animals themselves (animal-based or encompassing performance

  20. A phantom pig abdomen as an alternative for testing robotic surgical systems: our experience.

    Science.gov (United States)

    Ristolainen, Asko; Colucci, Gianluca; Kruusmaa, Maarja

    2013-11-01

    The use of animals for testing and validating new medical devices and surgical techniques has raised ethical issues for a long time. Following the introduction of the Three Rs principle, significant efforts have been made to achieve a reduction in the numbers of animals used in testing. Nevertheless, the number of large animals used for testing purposes is still too high. This article describes a potential alternative to the use of large animals in the early phase of the development of surgical equipment -- a high-definition phantom pig abdomen. The phantom pig abdomen was developed from computed tomography scans by using affordable materials, and it was used with two different robotic platforms. It permitted the testing of minimally-invasive robotic pancreatic enucleation, with or without intraoperative ultrasound guidance. The phantom pig abdomen has proven to be a realistic tool, with the potential to reduce the cost and time-frame of the experiments. 2013 FRAME.

  1. A determination and comparison of urease activity in feces and fresh manure from pig and cattle in relation to ammonia production and pH changes.

    Directory of Open Access Journals (Sweden)

    Xiaorong Dai

    Full Text Available Ammonia emission from animal production is a major environmental problem and has impacts on the animal health and working environment inside production houses. Ammonia is formed in manure by the enzymatic degradation of urinary urea and catalyzed by urease that is present in feces. We have determined and compared the urease activity in feces and manure (a urine and feces mixture from pigs and cattle at 25°C by using Michaelis-Menten kinetics. To obtain accurate estimates of kinetic parameters Vmax and K'm, we used a 5 min reaction time to determine the initial reaction velocities based on total ammoniacal nitrogen (TAN concentrations. The resulting Vmax value (mmol urea hydrolyzed per kg wet feces per min was 2.06±0.08 mmol urea/kg/min and 0.80±0.04 mmol urea/kg/min for pig feces and cattle feces, respectively. The K'm values were 32.59±5.65 mmol urea/l and 15.43±2.94 mmol urea/l for pig feces and cattle feces, respectively. Thus, our results reveal that both the Vmax and K'm values of the urease activity for pig feces are more than 2-fold higher than those for cattle feces. The difference in urea hydrolysis rates between animal species is even more significant in fresh manure. The initial velocities of TAN formation are 1.53 mM/min and 0.33 mM/min for pig and cattle manure, respectively. Furthermore, our investigation shows that the maximum urease activity for pig feces occurs at approximately pH 7, and in cattle feces it is closer to pH 8, indicating that the predominant fecal ureolytic bacteria species differ between animal species. We believe that our study contributes to a better understanding of the urea hydrolysis process in manure and provides a basis for more accurate and animal-specific prediction models for urea hydrolysis rates and ammonia concentration in manures and thus can be used to predict ammonia volatilization rates from animal production.

  2. Fermentation of liquid diets for pigs

    NARCIS (Netherlands)

    Scholten, R.

    2001-01-01

    In the last 30 years major changes occurred in pig nutrition. With the increasing size of the pig farms, manual feeding was replaced by automatic feeding systems. Most pigs were fed on dry diets, but during last the 15 years a clear tendency towards liquid feeding systems was observed. In the early

  3. Solar based hydrogen production systems

    CERN Document Server

    Dincer, Ibrahim

    2013-01-01

    This book provides a comprehensive analysis of various solar based hydrogen production systems. The book covers first-law (energy based) and second-law (exergy based) efficiencies and provides a comprehensive understanding of their implications. It will help minimize the widespread misuse of efficiencies among students and researchers in energy field by using an intuitive and unified approach for defining efficiencies. The book gives a clear understanding of the sustainability and environmental impact analysis of the above systems. The book will be particularly useful for a clear understanding

  4. Neurogastroenterology of tegaserod (HTF 919) in the submucosal division of the guinea-pig and human enteric nervous system.

    Science.gov (United States)

    Fang, X; Liu, S; Wang, X-Y; Gao, N; Hu, H-Z; Wang, G-D; Cook, C H; Needleman, B J; Mikami, D J; Xia, Y; Fei, G-J; Hicks, G A; Wood, J D

    2008-01-01

    Actions of the 5-HT(4) serotonergic receptor partial agonist, tegaserod, were investigated on mucosal secretion in the guinea-pig and human small intestine and on electrophysiological behaviour of secretomotor neurons in the guinea-pig small intestinal submucosal plexus. Expression of 5-HT(4) receptor protein and immunohistochemical localization of the 5-HT(4) receptor in the submucosal plexus in relation to expression and localization of choline acetyltransferase and the vesicular acetylcholine (ACh) transporter were determined for the enteric nervous system of human and guinea-pig small intestine. Immunoreactivity for the 5-HT(4) receptor was expressed as ring-like fluorescence surrounding the perimeter of the neuronal cell bodies and co-localized with the vesicular ACh transporter. Exposure of mucosal/submucosal preparations to tegaserod in Ussing chambers evoked increases in mucosal secretion reflected by stimulation of short-circuit current. Stimulation of secretion had a relative high EC(50) of 28.1 +/- 1.3 mumol L(-1), was resistant to neural blockade and appeared to be a direct action on the secretory epithelium. Tegaserod acted at presynaptic 5-HT(4) receptors to facilitate the release of ACh at nicotinic synapses on secretomotor neurons in the submucosal plexus. The 5-HT(2B) receptor subtype was not involved in actions at nicotinic synapses or stimulation of secretion.

  5. Determination of neophytadiene in the subcutaneous fat of Iberian pigs from different feeding systems

    Directory of Open Access Journals (Sweden)

    García-Casco, J. M.

    2013-04-01

    Full Text Available This study was carried out in order to determine the relationship between the content of a branched hydrocarbon (neophytadiene in the subcutaneous fat and the feeding system of Iberian pigs during the fattening period previous to slaughter. Thirty-six batches of Iberian pigs, belonging to the four categories of feeding systems described in the Spanish legislation (Bellota, Recebo, Campo and Cebo were studied. These categories differ in their degree of utilization of the natural resources (acorn and pasture of the extensive rearing system or in their level of replacement by concentrated feed. SPE combined with GC-MS techniques were carried out to isolate and to identify hydrocarbons. Recebo and Bellota batches showed the highest levels of neophytadiene content, followed by Campo batches, and finally, batches of the Cebo category. The results showed a great variability between batches from the same feeding category, mainly from Bellota, Recebo and Campo categories, which makes the classification of the animals in these three categories difficult. However, the animals from the Cebo category were correctly classified when reared in intensive conditions.El objetivo del trabajo se centró en determinar el contenido de un hidrocarburo ramificado (neofitadieno en la grasa subcutánea del cerdo ibérico, y su relación con el tipo de alimentación recibida durante la etapa de cebo previa al sacrificio (en la fase final de cebo. Para ello, se estudiaron treinta y seis lotes de cerdos Ibéricos, pertenecientes a las cuatro categorías de calidad en base a la alimentación de los animales establecidas en la Norma de Calidad (Bellota, Recebo, Campo y Cebo. Estas categorías se diferencian en el grado de aprovechamiento de los recursos naturales de la dehesa (bellotas y hierba y en el nivel de sustitución de los mismos por piensos concentrados. Para el aislamiento e identificación de los hidrocarburos se utilizaron técnicas de SPE combinadas con GC-MS. Los

  6. Efficient production by sperm-mediated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation

    Science.gov (United States)

    Lavitrano, Marialuisa; Bacci, Maria Laura; Forni, Monica; Lazzereschi, Davide; Di Stefano, Carla; Fioretti, Daniela; Giancotti, Paola; Marfé, Gabriella; Pucci, Loredana; Renzi, Luigina; Wang, Hongjun; Stoppacciaro, Antonella; Stassi, Giorgio; Sargiacomo, Massimo; Sinibaldi, Paola; Turchi, Valeria; Giovannoni, Roberto; Della Casa, Giacinto; Seren, Eraldo; Rossi, Giancarlo

    2002-01-01

    A large number of hDAF transgenic pigs to be used for xenotransplantation research were generated by using sperm-mediated gene transfer (SMGT). The efficiency of transgenesis obtained with SMGT was much greater than with any other method. In the experiments reported, up to 80% of pigs had the transgene integrated into the genome. Most of the pigs carrying the hDAF gene transcribed it in a stable manner (64%). The great majority of pigs that transcribed the gene expressed the protein (83%). The hDAF gene was transmitted to progeny. Expression was stable and found in caveolae as it is in human cells. The expressed gene was functional based on in vitro experiments performed on peripheral blood mononuclear cells. These results show that our SMGT approach to transgenesis provides an efficient procedure for studies involving large animal models. PMID:12393815

  7. Infected peripheral blood mononuclear cells transmit latent varicella zoster virus infection to the guinea pig enteric nervous system.

    Science.gov (United States)

    Gan, Lin; Wang, Mingli; Chen, Jason J; Gershon, Michael D; Gershon, Anne A

    2014-10-01

    Latent wild-type (WT) and vaccine (vOka) varicella zoster virus (VZV) are found in the human enteric nervous system (ENS). VZV also infects guinea pig enteric neurons in vitro, establishes latency and can be reactivated. We therefore determined whether lymphocytes infected in vitro with VZV secrete infectious virions and can transfer infection in vivo to the ENS of recipient guinea pigs. T lymphocytes (CD3-immunoreactive) were preferentially infected following co-culture of guinea pig or human peripheral blood mononuclear cells with VZV-infected HELF. VZV proliferated in the infected T cells and expressed immediate early and late VZV genes. Electron microscopy confirmed that VZV-infected T cells produced encapsulated virions. Extracellular virus, however, was pleomorphic, suggesting degradation occurred prior to release, which was confirmed by the failure of VZV-infected T cells to secrete infectious virions. Intravenous injection of WT- or vOka-infected PBMCs, nevertheless, transmitted VZV to recipient animals (guinea pig > human lymphocytes). Two days post-inoculation, lung and liver, but not gut, contained DNA and transcripts encoding ORFs 4, 40, 66 and 67. Twenty-eight days after infection, gut contained DNA and transcripts encoding ORFs 4 and 66 but neither DNA nor transcripts could any longer be found in lung or liver. In situ hybridization revealed VZV DNA in enteric neurons, which also expressed ORF63p (but not ORF68p) immunoreactivity. Observations suggest that VZV infects T cells, which can transfer VZV to and establish latency in enteric neurons in vivo. Guinea pigs may be useful for studies of VZV pathogenesis in the ENS.

  8. Production of α1,3-galactosyltransferase targeted pigs using transcription activator-like effector nuclease-mediated genome editing technology

    Science.gov (United States)

    Kang, Jung-Taek; Kwon, Dae-Kee; Park, A-Rum; Lee, Eun-Jin; Yun, Yun-Jin; Ji, Dal-Young; Lee, Kiho

    2016-01-01

    Recent developments in genome editing technology using meganucleases demonstrate an efficient method of producing gene edited pigs. In this study, we examined the effectiveness of the transcription activator-like effector nuclease (TALEN) system in generating specific mutations on the pig genome. Specific TALEN was designed to induce a double-strand break on exon 9 of the porcine α1,3-galactosyltransferase (GGTA1) gene as it is the main cause of hyperacute rejection after xenotransplantation. Human decay-accelerating factor (hDAF) gene, which can produce a complement inhibitor to protect cells from complement attack after xenotransplantation, was also integrated into the genome simultaneously. Plasmids coding for the TALEN pair and hDAF gene were transfected into porcine cells by electroporation to disrupt the porcine GGTA1 gene and express hDAF. The transfected cells were then sorted using a biotin-labeled IB4 lectin attached to magnetic beads to obtain GGTA1 deficient cells. As a result, we established GGTA1 knockout (KO) cell lines with biallelic modification (35.0%) and GGTA1 KO cell lines expressing hDAF (13.0%). When these cells were used for somatic cell nuclear transfer, we successfully obtained live GGTA1 KO pigs expressing hDAF. Our results demonstrate that TALEN-mediated genome editing is efficient and can be successfully used to generate gene edited pigs. PMID:27051344

  9. Production of α1,3-galactosyltransferase targeted pigs using transcription activator-like effector nuclease-mediated genome editing technology.

    Science.gov (United States)

    Kang, Jung-Taek; Kwon, Dae-Kee; Park, A-Rum; Lee, Eun-Jin; Yun, Yun-Jin; Ji, Dal-Young; Lee, Kiho; Park, Kwang-Wook

    2016-03-01

    Recent developments in genome editing technology using meganucleases demonstrate an efficient method of producing gene edited pigs. In this study, we examined the effectiveness of the transcription activator-like effector nuclease (TALEN) system in generating specific mutations on the pig genome. Specific TALEN was designed to induce a double-strand break on exon 9 of the porcine α1,3-galactosyltransferase (GGTA1) gene as it is the main cause of hyperacute rejection after xenotransplantation. Human decay-accelerating factor (hDAF) gene, which can produce a complement inhibitor to protect cells from complement attack after xenotransplantation, was also integrated into the genome simultaneously. Plasmids coding for the TALEN pair and hDAF gene were transfected into porcine cells by electroporation to disrupt the porcine GGTA1 gene and express hDAF. The transfected cells were then sorted using a biotin-labeled IB4 lectin attached to magnetic beads to obtain GGTA1 deficient cells. As a result, we established GGTA1 knockout (KO) cell lines with biallelic modification (35.0%) and GGTA1 KO cell lines expressing hDAF (13.0%). When these cells were used for somatic cell nuclear transfer, we successfully obtained live GGTA1 KO pigs expressing hDAF. Our results demonstrate that TALEN-mediated genome editing is efficient and can be successfully used to generate gene edited pigs.

  10. Genomics and systems biology of boar taint and meat quality in pigs

    DEFF Research Database (Denmark)

    Drag, Markus; Kogelman, Lisette; Meinert, Lene

    2015-01-01

    , economic losses associated with castrated pigs and a ban on castration in the EU effective by 2018. The main objective of the PhD project is to unravel the underlying mechanisms of BT at the genomic, transcriptomic and phenotypic levels as well as its connection with sensory meat quality (SMQ) in order...... of ~52. Gene expression levels were quantified by HTseq. Genotypic profiling by 60K Porcine SNPchip was performed and selected cuts from fore-end, loin, ham and backfat of the pigs was subjected to SMQ assessments. Combining SNPchip, RNA-seq and SMQ data will reveal causative genes, co...

  11. Simulation of the energy performance of maize production integrated to pig farming Simulação do desempenho energético da produção de milho integrada à suinocultura

    Directory of Open Access Journals (Sweden)

    Márcio L. Perin

    2012-10-01

    Full Text Available The study evaluated the energy performance of pig farming integrated with maize production in mechanized no-tillage system. In this proposed conception of integration, the swine excrement is used as fertilizers in the maize crop. The system was designed involving the activities associated to the pig management and maize production (soil management, cultivation and harvest. A one-year period of analysis was considered, enabling the production of three batches of pigs and two crops of maize. To evaluate the energy performance, three indicators were created: energy efficiency, use of non-renewable resources efficiency and cost of non-renewable energy to produce protein. The energy inputs are composed by the inputs and infrastructure used by the breeding of pigs and maize production, as well as the solar energy incident on the agroecosystem. The energy outputs are represented by the products (finished pigs and maize. The results obtained in the simulation indicates that the integration improves the energy performance of pig farms, with an increase in the energy efficiency (186% as well as in the use of the non-renewable energy resources efficiency (352%, while reducing the cost of non-renewable energy to produce protein (‑58%.Foi avaliado o desempenho energético da suinocultura integrada à produção de milho em grão em sistema de plantio direto mecanizado. Nesta concepção de integração proposta, os dejetos suínos são utilizados como fertilizantes na produção de milho. O sistema foi delimitado envolvendo as atividades associadas ao manejo dos suínos e de produção do milho (manejo do solo, cultivo e colheita. O período de análise considerado foi de um ano, o que possibilita a produção de três lotes de suínos e duas safras de milho. Para avaliar o desempenho energético, foram criados três indicadores: eficiência energética, eficiência de uso de fontes não renováveis e o custo de energia não renovável para a produção de

  12. Expression patterns of insulin-like growth factor system members and their correlations with growth and carcass traits in Landrace and Lantang pigs during postnatal development.

    Science.gov (United States)

    Li, Zicong; Wu, Zhenfang; Ren, Guangcai; Zhao, Yunxiang; Liu, Dewu

    2013-05-01

    The IGF system plays important roles in growth. Nevertheless, few data have been reported so far on the expression of IGF system members and their relationship with growth in domestic animals, especially pigs. In this study, hepatic transcript level of IGF1, IGF2, IGF binding protein 2 (IGFBP2), IGFBP3 and IGF 1 receptor (IGF1R), plasma protein level of IGF1 and IGFBP3, and eight growth or carcass traits, including chest circumference, body length, body height (BH), body weight, carcass weight, loin muscle area (LMA), back fat thickness and average daily gain, were measured in fast-growing Landrace and slow-growing Lantang pigs at the age of 1, 27, 90, 150 and 180 days. The results showed that liver mRNA level of IGF1, IGF2 and IGF1R, and blood protein level of IGF1 have a similar developmental profile in both Landrace and Lantang pigs. Their levels were higher at the early age than that at other older ages. Hepatic transcript abundances of the two growth inhibitors, IGFBP2 and IGFBP3, were mostly higher in Lantang pigs than that in Landrace pigs, at 5 examined postnatal stages. The IGF system members' liver mRNA level and/or serum protein level have significant correlation with each other in different age of Landrace or Lantang pigs. Hepatic mRNA level or serum protein level of IGF system members also has significant correlation with investigated traits, especially with BH and LMA, in different age of Lantang or Landrace pigs. Our results revealed the change profiles of porcine IGF system members' liver transcript level and plasma protein level between different pig breeds and different postnatal developmental ages. Moreover, the correlation analysis results suggest that the IGF system members act coordinately to regulate the growth performance and carcass composition in pigs. The information obtained from the present study is important for elucidation of the regulatory mechanism of IGF system underlying growth, and for genetic improvement in pigs.

  13. A selective genotyping approach identifies single nucleotide polymorphisms in porcine chromosome 2 genes associated with production and carcass traits in Italian heavy pigs

    Directory of Open Access Journals (Sweden)

    Vincenzo Russo

    2011-04-01

    Full Text Available Several studies have shown that porcine chromosome 2 (SSC2 harbors important quantitative trait loci (QTL for production traits. In particular, an imprinted QTL for muscle mass production is determined by a mutation in the IGF2 gene (intron3-g.3072G>A. We recently identified and analysed single nucleotide polymorphisms (SNPs in genes (cathepsin D, CTSD g.70G>A; cathepsin F, CTSF g.22G>C; lactate dehydrogenase A, LDHA g.46G>T localized on SSC2 (including the IGF2 intron3-g.3072G>A SNP showing association with production traits in Italian Large White pigs and/or localizing them on QTL regions. Here we analysed these markers applying a selective genotyping approach based on estimated breeding values (EBVs. Three groups of Italian Large White pigs each made by animals with the most positive (n. 50 and most negative (n. 50 EBVs for average daily gain (ADG, backfat thickness (BFT or weight of lean cuts (LC and one group of Italian Duroc pigs made by 50 animals with most positive and 50 animals with most negative EBV for visible intermuscular fat (VIF were genotyped. In Italian Large White pigs, allele frequency differences for the IGF2 intron3-g.3072G>A SNP between the two extreme tails for all groups were highly significant (considering all analysed animals: P=9.53E-20 for LC; P=3.16E-15 for BFT; P=4.41E-6 for ADG. Significant allele frequency differences were also observed for the CTSD g.70G>A (P=0.0002 for ADG; P=0.00068 and LDHA g.46G>T (P=2.32E-5 for ADG polymorphisms. These results provide further support on the effects of these polymorphisms or genes whose application on marker assisted selection programs could be envisaged.

  14. Differential expression of canonical (classical) transient receptor potential channels in guinea pig enteric nervous system.

    Science.gov (United States)

    Liu, Sumei; Qu, Mei-Hua; Ren, Wei; Hu, Hong-Zhen; Gao, Na; Wang, Guo-Du; Wang, Xi-Yu; Fei, Guijun; Zuo, Fei; Xia, Yun; Wood, Jackie D

    2008-12-20

    The canonical transient receptor potential (TRPC) family of ion channels is implicated in many neuronal processes including calcium homeostasis, membrane excitability, synaptic transmission, and axon guidance. TRPC channels are postulated to be important in the functional neurobiology of the enteric nervous system (ENS); nevertheless, details for expression in the ENS are lacking. Reverse transcriptase-polymerase chain reaction, Western blotting, and immunohistochemistry were used to study the expression and localization of TRPC channels. We found mRNA transcripts, protein on Western blots, and immunoreactivity (IR) for TRPC1/3/4/6 expressed in the small intestinal ENS of adult guinea pigs. TRPC1/3/4/6-IR was localized to distinct subpopulations of enteric neurons and was differentially distributed between the myenteric and submucosal divisions of the ENS. TRPC1-IR was widely distributed and localized to neurons with cholinergic, calretinin, and nitrergic neuronal immunochemical codes in the myenteric plexus. It was localized to both cholinergic and noncholinergic secretomotor neurons in the submucosal plexus. TRPC3-IR was found only in the submucosal plexus and was expressed exclusively by neuropeptide Y-IR neurons. TRPC4/6-IR was expressed in only a small population of myenteric neurons, but was abundantly expressed in the submucosal plexus. TRPC4/6-IR was coexpressed with both cholinergic and nitrergic neurochemical codes in the myenteric plexus. In the submucosal plexus, TRPC4/6-IR was expressed exclusively in noncholinergic secretomotor neurons. No TRPC1/3/4/6-IR was found in calbindin-IR neurons. TRPC3/4/6-IR was widely expressed along varicose nerve fibers and colocalized with synaptophysin-IR at putative neurotransmitter release sites. Our results suggest important roles for TRPC channels in ENS physiology and neuronal regulation of gut function.

  15. Impact of animal health management on organic pig farming in Greece

    OpenAIRE

    Papatsiros V.G.

    2011-01-01

    In Greece, organic pig farming started in 2002 and since then made significant steps forward due to the extended interesting of Greek consumer for organic products during last decade. This report aims at updating information about organic pig farming in Greece, relating production system and most health risk factors. Furthermore, in present study a animal health management program is proposed. The most common health problems that occur in the Greek organic ...

  16. Ultra-Deepwater Production Systems

    Energy Technology Data Exchange (ETDEWEB)

    Ken L. Smith; Marc E. Leveque

    2005-05-31

    The report herein is a summary of the work performed on three projects to demonstrate hydrocarbon drilling and production methods applicable to deep and ultra deepwater field developments in the Gulf of Mexico and other like applications around the world. This work advances technology that could lead to more economic development and exploitation of reserves in ultra-deep water or remote areas. The first project is Subsea Processing. Its scope includes a review of the ''state of the art'' in subsea components to enable primary production process functions such as first stage liquids and gas separation, flow boosting, chemical treatment, flow metering, etc. These components are then combined to allow for the elimination of costly surface production facilities at the well site. A number of studies were then performed on proposed field development projects to validate the economic potential of this technology. The second project involved the design and testing of a light weight production riser made of composite material. The proposed design was to meet an actual Gulf of Mexico deepwater development project. The various engineering and testing work is reviewed, including test results. The third project described in this report encompasses the development and testing of a close tolerance liner drilling system, a new technology aimed at reducing deepwater drilling costs. The design and prototype testing in a test well are described in detail.

  17. Socio-cultural sustainability of pig production: Citizen perceptions in the Netherlands and Denmark.

    NARCIS (Netherlands)

    Boogaard, B.K.; Boekhorst, L.J.S.; Oosting, S.J.; Sorensen, J.T.

    2011-01-01

    Many sustainability studies of animal production consider three pillars: the economic, environmental and socio-cultural. Farmers and animal scientists tend to put most emphasis on the economic and environmental pillar and largely ignore the socio-cultural pillar. Socio-cultural sustainability refers

  18. Development of a genetic tool for product regulation in the diverse British pig breed market

    NARCIS (Netherlands)

    Wilkinson, H.S.; Archibald, A.L.; Haley, C.S.; Megens, H.J.W.C.; Crooijmans, R.P.M.A.; Groenen, M.

    2012-01-01

    Background - The application of DNA markers for the identification of biological samples from both human and non-human species is widespread and includes use in food authentication. In the food industry the financial incentive to substituting the true name of a food product with a higher value alter

  19. Development of a genetic tool for product regulation in the diverse British pig breed market

    NARCIS (Netherlands)

    Wilkinson, H.S.; Archibald, A.L.; Haley, C.S.; Megens, H.J.W.C.; Crooijmans, R.P.M.A.; Groenen, M.

    2012-01-01

    Background - The application of DNA markers for the identification of biological samples from both human and non-human species is widespread and includes use in food authentication. In the food industry the financial incentive to substituting the true name of a food product with a higher value

  20. Sterile Product Packaging and Delivery Systems.

    Science.gov (United States)

    Akers, Michael J

    2015-01-01

    Both conventional and more advanced product container and delivery systems are the focus of this brief article. Six different product container systems will be discussed, plus advances in primary packaging for special delivery systems and needle technology.

  1. Evidence for a role of CCK as neurotransmitter in the guinea-pig enteric nervous system.

    NARCIS (Netherlands)

    Schutte, I.W.M.; Hollestein, K.B.C.W.; Akkermans, L.M.A.; Kroese, A.B.A.

    1997-01-01

    Intracellular recordings were made of neurons in the myenteric plexus of the guinea-pig distal ileum. Slow excitatory post-synaptic potentials (sEPSPs) were evoked by electrical stimulation of an interganglionic fibre tract. The effect of cholecystokinin (CCK) receptor antagonists on the sEPSPs was

  2. Infectious and rearing-system related risk factors for chronic pleuritis in slaughter pigs

    DEFF Research Database (Denmark)

    Enøe, Claes; Mousing, Jan; Schirmer, Anna Luise

    2002-01-01

    Chronic pleuritis (CP) in Danish pigs for slaughter is by far the most frequent finding at the routine post-mortem meat inspection. An initial investigation published in 1990 demonstrated infectious and management-related risk factors. Serological testing for additional infectious agents, as well...

  3. Behaviour of liquid-fed growing pigs provided with straw in various amounts and frequencies.

    Science.gov (United States)

    Oxholm, L C; Steinmetz, H V; Lahrmann, H P; Nielsen, M B F; Amdi, C; Hansen, C F

    2014-11-01

    Straw possesses many characteristics that make it attractive to pigs and can therefore be effective in preventing negative penmate-directed behaviours. However, straw is difficult to handle in current vacuum slurry systems under most commercial conditions and can therefore only be used in limited amounts. To occupy pigs effectively, straw must remain attractive to pigs throughout the whole day; hence, have a certain degree of novelty. We investigated the penmate-directed behaviour of liquid-fed growing pigs in a production herd, assigned to five experimental treatments: 1×25, 1×50, 1×100, 2×50 and 4×25 g of chopped straw/pig per day, with 20 replicates of each treatment (pen was regarded as experimental unit). Behaviour was observed at two different growth stages; ~40 and 80 kg live weight of the pigs. Activity and exploratory behaviour directed at penmates, straw, pen components and the slatted floor were registered continuously for 15 min of each hour during day time (0600 to 2200 h) by use of video observation of three focal pigs per pen. The pigs were active for about one-third of the day corresponding to ~5 h/day. Of the active time, an average of 7% (35 min) was spent on penmate-directed behaviour. The pigs were more active and increased their straw-directed behaviour when provided with 100 g straw/pig per day compared with 25 and 50 g (Pstraw (P>0.05), and there was no effect on pigs' behaviour when straw provision was increased per day (P>0.05). Pigs became less active and reduced their straw-directed activities when their weight increased from 40 to 80 kg live weight (P0.05). Further, the residual straw results indicated that perhaps a more frequent straw provision could help establish a more even level of fresh available straw during the day. However, the frequent straw provision did not occupy pigs more than one daily allocation did. In conclusion, there was no difference in penmate-directed behaviour of the pigs when given 25 or 50 g of straw/pig

  4. Nutritional value of dried fermentation biomass, hydrolyzed porcine intestinal mucosa products, and fish meal fed to weanling pigs.

    Science.gov (United States)

    Sulabo, R C; Mathai, J K; Usry, J L; Ratliff, B W; McKilligan, D M; Moline, J D; Xu, G; Stein, H H

    2013-06-01

    Dried fermentation biomass (DFB) and hydrolyzed porcine intestinal mucosa are co-products of L-Lys • HCl production and heparin extraction, respectively. Three experiments were conducted to determine standardized ileal digestibility (SID) of AA (Exp. 1), concentration of DE and ME (Exp. 2), and standardized total tract digestibility (STTD) of P (Exp. 3) in DFB and 2 hydrolyzed porcine intestinal mucosa products (PEP50 and PEP2+), and compare these values with values for fish meal. In Exp. 1, 12 ileal cannulated barrows (BW = 11.5 ± 1.1 kg) were allotted to a replicated 6 × 6 Latin square design with 6 diets and 6 periods. A N-free diet, diet based on soybean meal (SBM), and 4 diets based on a combination of SBM and DFB, PEP50, PEP2+, or fish meal were formulated. With the exception of Lys, there were no differences in SID of indispensable AA between DFB and fish meal. Except for Thr, no differences in SID of indispensable AA between PEP50 and fish meal were observed, but SID of all indispensable AA, except Lys and Trp, was less (P < 0.05) in PEP2+ than in the other ingredients. In Exp. 2, 40 barrows (BW = 12.8 ± 1.4 kg) were allotted to 5 diets with 8 pigs/diet. A basal diet containing 96.4% corn and 4 diets containing corn and DFB, PEP50, PEP2+, or fish meal were formulated. The DE (5,445 kcal/kg DM) and ME (5,236 kcal/kg DM) in DFB were greater (P < 0.01) than in PEP50 (4,758 and 4,512 kcal/kg DM for DE and ME, respectively) and fish meal (4,227 and 3,960 kcal/kg DM for DE and ME, respectively). Also, DE in DFB was greater (P < 0.01) than in PEP2+ (4,935 kcal/kg DM), but ME in DFB was not different from that in PEP2+ (4,617 kcal/kg DM). Furthermore, DE in PEP50 and PEP2+ were greater (P < 0.01) than in fish meal, but ME did not differ from that in fish meal. In Exp. 3, 40 barrows (BW = 12.4 ± 1.3 kg) were randomly allotted to 5 diets with 8 pigs/diet. A P-free diet and 4 diets in which the sole source of P was from DFB, PEP50, PEP2+, or fish meal were

  5. Optimised biogas production from the co-digestion of sugar beet with pig slurry: Integrating energy, GHG and economic accounting

    DEFF Research Database (Denmark)

    Boldrin, Alessio; Baral, Khagendra Raj; Fitamo, Temesgen Mathewos

    2016-01-01

    Several countries have established a number of increased targets for energy production from renewable sources. Biogas production, which will play a key role in future energy systems largely based on renewable sources, is expected to grow significantly in the next few decades. To achieve these amb......Several countries have established a number of increased targets for energy production from renewable sources. Biogas production, which will play a key role in future energy systems largely based on renewable sources, is expected to grow significantly in the next few decades. To achieve...

  6. Greenhouse production systems for people

    NARCIS (Netherlands)

    Giacomelli, G.A.; Sase, S.; Cramer, R.; Hoogeboom, J.; McKenzie, A.; Parbst, K.; Sacrascia-Mugnozza, G.; Selina, P.; Sharp, D.A.; Voogt, J.O.; Weel, van P.A.; Mears, D.

    2012-01-01

    Environmentally sound greenhouse production requires that: demand for market products is understood; greenhouse design addresses the climate circum-stances; input resources are available and consumed efficiently, and; there must be a reasonable balance of production products to the environmental

  7. Input management of production systems.

    Science.gov (United States)

    Odum, E P

    1989-01-13

    Nonpoint sources of pollution, which are largely responsible for stressing regional and global life-supporting atmosphere, soil, and water, can only be reduced (and ultimately controlled) by input management that involves increasing the efficiency of production systems and reducing the inputs of environmentally damaging materials. Input management requires a major change, an about-face, in the approach to management of agriculture, power plants, and industries because the focus is on waste reduction and recycling rather than on waste disposal. For large-scale ecosystem-level situations a top-down hierarchical approach is suggested and illustrated by recent research in agroecology and landscape ecology.

  8. Ultra-Deepwater Production Systems

    Energy Technology Data Exchange (ETDEWEB)

    K. L. Smith; M. E. Leveque

    2004-09-30

    This report includes technical progress made during the period October, 2003 through September, 2004. At the end of the last technical progress report, the subsea processing aspects of the work program had been dropped due to the lack of commercial opportunity within ConocoPhillips, and the program had been redirected towards two other promising deepwater technologies: the development and demonstration of a composite production riser, and the development and testing of a close-tolerance liner drilling system. This report focuses on these two technologies.

  9. Prevalence of gastrointestinal nematodes in growing pigs in Kabale District in Uganda.

    Science.gov (United States)

    Nissen, Sofie; Poulsen, Idahella H; Nejsum, Peter; Olsen, Annette; Roepstorff, Allan; Rubaire-Akiiki, C; Thamsborg, Stig M

    2011-03-01

    During the last 30 years, pig production in Uganda and neighbouring counties has increased markedly. Pigs are mainly kept as a source of income for small-scale farmers; however, the pig production is subject to several constraints, one of them being worm infections. A study was carried out in rural communities in Kabale District in the South Western part of Uganda in September and October 2007 in order to estimate the prevalence of gastrointestinal nematode parasites in pigs based on coprological examination. Fifty-six households were randomly selected and visited. Housing system and deworming history were recorded. Faeces was sampled from rectum of one to five pigs (age, 3-12 months) per household. A total of 106 pigs were examined coprologically of which 91% excreted nematode eggs. The following prevalences of nematode eggs were recorded: strongyles (89%), Ascaris suum (40%), Trichuris suis (17%) and spiruroid eggs (48%). On household level, rearing pigs on slatted floors in pens significantly reduced the faecal egg excretion of strongyle eggs with almost 80% (p=0.010) and a significant interaction between floor type and anthelmintic treatment was found for spiruroids (p=0.037). Fifteen T. suis egg positive pigs were selected for post-mortem examination of the gastrointestinal tract. The post-mortem examinations revealed that 93% pigs were infected with Oesophagostomum spp. (worm burden, min-max 10-2,180), 73% with A. suum (1-36), 67% with T. suis (6-58), and 20% with Hyostrongylus rubidus (worms not quantified). In general, nematode infections were widespread and polyparasitism common in pigs in Uganda. However, worm burdens were moderate which may be related to recent deworming or to the practice of rearing pigs on slatted floors in wooden elevated pens.

  10. Effects of fermentation products of Ganoderma lucidum on growth performance and immunocompetence in weanling pigs.

    Science.gov (United States)

    Chen, Su-Der; Hsieh, Meng-Chen; Chiou, Ming-Tang; Lai, Yu-Shen; Cheng, Yeong-Hsiang

    2008-02-01

    The purpose of this study was to test fermentation, for its products of a Chinese medicinal mushroom, Ganoderma lucidum, cultured by submerged fermentation for its effect on growth performance and immunocompetence in weanling piglets. In Experiment 1, 72 weanling piglets were allotted to one of four treatments receiving these fermentation products (GLF, expressed as amount of beta-glucans) at 0 (control), 50, 100, and 150 mg/kg feed for 4 weeks. The results showed that at a supplementation level of 50 mg/kg feed, GLF caused the best growth performance, the highest pseudorabies antibody titre, and a decrease of blood glucose level. It was also demonstrated that GLF up-regulated the cell-mediated immune response related cytokines (IL-2, IFN-gamma, and TNF-alpha) expression in different lymphoid tissues. After challenging with porcine circovirus (PCV) type 2 (Experiment 2), a supplementation with 50 mg GLF per kg feed also inhibited PCV-2 virus amplification, and ameliorated lymphocyte depletion in different lymphoid tissues. Conclusively, feed supplemented with GLF at 50 mg/kg could be beneficial to counteract the physiological stress in weanling piglets.

  11. Nonstarch polysaccharides modulate bacterial microbiota, pathways for butyrate production, and abundance of pathogenic Escherichia coli in the pig gastrointestinal tract.

    Science.gov (United States)

    Metzler-Zebeli, Barbara U; Hooda, Seema; Pieper, Robert; Zijlstra, Ruurd T; van Kessel, Andrew G; Mosenthin, Rainer; Gänzle, Michael G

    2010-06-01

    The impact of nonstarch polysaccharides (NSP) differing in their functional properties on intestinal bacterial community composition, prevalence of butyrate production pathway genes, and occurrence of Escherichia coli virulence factors was studied for eight ileum-cannulated growing pigs by use of terminal restriction fragment length polymorphism (TRFLP) and quantitative PCR. A cornstarch- and casein-based diet was supplemented with low-viscosity, low-fermentability cellulose (CEL), with high-viscosity, low-fermentability carboxymethylcellulose (CMC), with low-viscosity, high-fermentability oat beta-glucan (LG), and with high-viscosity, high-fermentability oat beta-glucan (HG). Only minor effects of NSP fractions on the ileal bacterial community were observed, but NSP clearly changed the digestion in the small intestine. Compared to what was observed for CMC, more fermentable substrate was transferred into the large intestine with CEL, LG, and HG, resulting in higher levels of postileal dry-matter disappearance. Linear discriminant analysis of NSP and TRFLP profiles and 16S rRNA gene copy numbers for major bacterial groups revealed that CMC resulted in a distinctive bacterial community in comparison to the other NSP, which was characterized by higher gene copy numbers for total bacteria, Bacteroides-Prevotella-Porphyromonas, Clostridium cluster XIVa, and Enterobacteriaceae and increased prevalences of E. coli virulence factors in feces. The numbers of butyryl-coenzyme A (CoA) CoA transferase gene copies were higher than those of butyrate kinase gene copies in feces, and these quantities were affected by NSP. The present results suggest that the NSP fractions clearly and distinctly affected the taxonomic composition and metabolic features of the fecal microbiota. However, the effects were more linked to the individual NSP and to their effect on nutrient flow into the large intestine than to their shared functional properties.

  12. Leak detector PIG for oil pipelines; PIG detector de vazamentos em oleodutos

    Energy Technology Data Exchange (ETDEWEB)

    Camerini, Daniel Almeida; Weid, Jean Pierre von der [Pontificia Univ. Catolica do Rio de Janeiro, RJ (Brazil). Centro de Estudos em Telecomunicacoes; Camerini, Claudio Soligo; Maia, Carlos Eduardo [PETROBRAS, Rio de Janeiro, RJ (Brazil). Centro de Pesquisas (CENPES)

    2003-07-01

    The leakage detector pig was created with the purpose of detect and locate leakages in a preventive way. The fast detection of small leakages decreases the probability of larger leakages. By means of a differential pressure system based on pressure data acquired through the pig's running, using pressure sensors installed on the pig's vessel, small leakages are identified and its positions registered. This allows a quick intervention at the identified location. The working principle is based on the pressure difference caused by the leakage, which is read by the sensors. The polyurethane cups responsible by the pig's propulsion are of a special kind capable of sealing a constant volume of product among them. So, when the pig passes through a leakage, the pressure in the middle drops compared to those in front and hear, generating a characteristic signal thus permit identification. The first prototype was tested intensively at laboratory facilities, and in a test pipeline showing excellent results locating precisely all the simulated leakages. Defects from different sizes with different flow where simulated and measured. This work has the objective to present the tool, its potential, the test results, to discuss its efficiency in detect and locate leakages and the possibilities for the future. (author)

  13. Growth of and valine production by a Bacillus subtilis mutant in the small intestine of pigs

    DEFF Research Database (Denmark)

    Canibe, Nuria; Poulsen, Henrik Vestergaard; Nørgaard, Jan Værum;

    2016-01-01

    :Lys of 0.63:1 (Neg), 2) the Neg diet with added Bacillus subtilis-valine (1.28 × 108 cfu/g feed) (+Bac), and 3) the Neg diet with added L-Val to a Val:Lys of 0.69:1 (+Val). Eighteen gilts (6 on each treatment) with initial weights of ∼15 kg were fed the diets for 23 d before the animals were euthanized...... and samples from the small intestine were obtained. The number of B. subtilis cfu in digesta was higher in the +Bac group than in the Neg group (P ... concentrations were measured in the +Bac group. Short-term in vitro incubations of digesta showed a decrease (P ≤ 0.03) in the number of B. subtilis cfu over time for the +Bac group and no difference in the rate of Val production compared to that in the Neg group. In conclusion, more B. subtilis cfu were present...

  14. Macrophage colony-stimulating factor (CSF1) controls monocyte production and maturation and the steady-state size of the liver in pigs.

    Science.gov (United States)

    Sauter, Kristin A; Waddell, Lindsey A; Lisowski, Zofia M; Young, Rachel; Lefevre, Lucas; Davis, Gemma M; Clohisey, Sara M; McCulloch, Mary; Magowan, Elizabeth; Mabbott, Neil A; Summers, Kim M; Hume, David A

    2016-09-01

    Macrophage colony-stimulating factor (CSF1) is an essential growth and differentiation factor for cells of the macrophage lineage. To explore the role of CSF1 in steady-state control of monocyte production and differentiation and tissue repair, we previously developed a bioactive protein with a longer half-life in circulation by fusing pig CSF1 with the Fc region of pig IgG1a. CSF1-Fc administration to pigs expanded progenitor pools in the marrow and selectively increased monocyte numbers and their expression of the maturation marker CD163. There was a rapid increase in the size of the liver, and extensive proliferation of hepatocytes associated with increased macrophage infiltration. Despite the large influx of macrophages, there was no evidence of liver injury and no increase in circulating liver enzymes. Microarray expression profiling of livers identified increased expression of macrophage markers, i.e., cytokines such as TNF, IL1, and IL6 known to influence hepatocyte proliferation, alongside cell cycle genes. The analysis also revealed selective enrichment of genes associated with portal, as opposed to centrilobular regions, as seen in hepatic regeneration. Combined with earlier data from the mouse, this study supports the existence of a CSF1-dependent feedback loop, linking macrophages of the liver with bone marrow and blood monocytes, to mediate homeostatic control of the size of the liver. The results also provide evidence of safety and efficacy for possible clinical applications of CSF1-Fc.

  15. Application of N-carbamylglutamate in Pig Production%N-氨甲酰谷氨酸在养猪生产中的应用

    Institute of Scientific and Technical Information of China (English)

    冯涛; 白佳桦; 闫学军; 许晓玲; 周海深; 康小有; 刘彦

    2012-01-01

    N-氨甲酰谷氨酸(N-carbamylglutamate,NCG)是一种新型的猪用饲料添加剂,在饲料中按一定比例添加可显著提高猪的繁殖性能和生产性能.N-氨甲酰谷氨酸(NCG)主要通过促进精氨酸的内源合成,提高母猪窝产仔数和初生重,增强公猪的繁殖力,促进仔猪的生长和发育.作者将NCG在养猪生产中的应用进行了综述,以期为提高养猪生产效率提供参考依据.%N-carbamylglutamate (NCG) is a kind of new pig feed additive, which can improve the reproductive performance including increasing the total piglets born per litter and the birth weight of all piglets born of sow, enhancing the fecundity of boar and promoting the growth and development of piglets through promoting the endogenous synthesis of arginine. The use of NCG in pig was reviewed to provide examples for hoggeries to improve their efficiency of pig production.

  16. Management practices associated with the carriage of Yersinia enterocolitica in pigs at farm level.

    Science.gov (United States)

    Vilar, María J; Virtanen, Sonja; Heinonen, Mari; Korkeala, Hannu

    2013-07-01

    Pigs are the most important reservoir of Yersinia enterocolitica infections in humans. Knowledge of farm management practices that contribute to the transmission of this bacterial species in pigs is essential to understand how to control this foodborne pathogen in food production. The prevalence of Y. enterocolitica, and other results obtained from an age trend analysis were used to estimate the on-farm risk of transmission of specific management practices for this pathogen in 30 pig farms in Finland. Log-linear analysis revealed that rearing pigs in pens without or with sparse amounts of bedding and buying piglets from more than one farm were the variables that contribute most to the occurrence of Y. enterocolitica. The study also found that using an all-in/all-out management system and supplying water of municipal origin were factors that might reduce the prevalence of Y. enterocolitica, and therefore the risk of transmission of Y. enterocolitica in pig farms.

  17. The mating behaviour and reproduction performance in a multi-sire mating system for pigs

    DEFF Research Database (Denmark)

    Kongsted, Anne Grete; Hermansen, John Erik

    2008-01-01

    An important aim of organic animal production is to allow natural animal behaviour. Regarding reproduction techniques, artificial insemination is permitted but natural mating is preferred. The outdoor multi-sire system, where the sows are placed in large paddocks with a group of boars, is one...

  18. Platelet-activating factor in the enteric nervous system of the guinea pig small intestine.

    Science.gov (United States)

    Wang, Guo-Du; Wang, Xi-Yu; Hu, Hong-Zhen; Fang, Xiu-Cai; Liu, Sumei; Gao, Na; Xia, Yun

    2006-11-01

    Platelet-activating factor (PAF) is a proinflammatory mediator that may influence neuronal activity in the enteric nervous system (ENS). Electrophysiology, immunofluorescence, Western blot analysis, and RT-PCR were used to study the action of PAF and the expression of PAF receptor (PAFR) in the ENS. PAFR immunoreactivity (IR) was expressed by 6.9% of the neurons in the myenteric plexus and 14.5% of the neurons in the submucosal plexus in all segments of the guinea pig intestinal tract as determined by double staining with anti-human neuronal protein antibody. PAFR IR was found in 6.1% of the neurons with IR for calbindin, 35.8% of the neurons with IR for neuropeptide Y (NPY), 30.6% of the neurons with IR for choline acetyltransferase (ChAT), and 1.96% of the neurons with IR for vasoactive intestinal peptide (VIP) in the submucosal plexus. PAFR IR was also found in 1.5% of the neurons with IR for calbindin, 51.1% of the neurons with IR for NPY, and 32.9% of the neurons with IR for ChAT in the myenteric plexus. In the submucosal plexus, exposure to PAF (200-600 nM) evoked depolarizing responses (8.2 +/- 3.8 mV) in 12.4% of the neurons with S-type electrophysiological behavior and uniaxonal morphology and in 12.5% of the neurons with AH-type electrophysiological behavior and Dogiel II morphology, whereas in the myenteric preparations, depolarizing responses were elicited by a similar concentration of PAF in 9.5% of the neurons with S-type electrophysiological behavior and uniaxonal morphology and in 12.0% of the neurons with AH-type electrophysiological behavior and Dogiel II morphology. The results suggest that subgroups of secreto- and musculomotor neurons in the submucosal and myenteric plexuses express PAFR. Coexpression of PAFR IR with ChAT IR in the myenteric plexus and ChAT IR and VIP IR in the submucosal plexus suggests that PAF, after release in the inflamed bowel, might act to elevate the excitability of submucosal secretomotor and myenteric musculomotor

  19. Effects of caloric vestibular stimulation on serotoninergic system in the media vestibular nuclei of guinea pigs

    Institute of Scientific and Technical Information of China (English)

    MA Fu-rong; LIU Jun-xiu; LI Xue-pei; MAO Jian-jun; ZHANG Qun-dan; JIA Hong-bo; MAO Lan-quan; ZHAO Rui

    2007-01-01

    Background Anatomic and electrophysiological studies have revealed that the neurons located in the media vestibular nuclei (MVN) receive most of the sensory vestibular input coming from the ipsilateral labyrinth and the responses of MVN neurons to caloric stimulation directly reflect changes in primary vestibular afferent activity. The aim of this study was to clarify the intrinsic characteristics of serotonin (5-hydroxytryptamine, 5-HT) release in the MVN during the period of vertigo induced by caloric stimulation.Methods We used an in vivo microdialysis technique to examine the effects of caloric stimulation on the serotoninergic system in MVN. Twenty four guinea pigs were randomly divided into the groups of irrigation of the ear canal with hot water (n=6), ice water (n=6) and 37℃ water (n=4), and the groups of irrigation of the auricle with hot water (n=4) and ice water (n=4), according to different caloric vestibular stimulation. We examined the animal's caloric nystagmus with a two-channel electronystagmographic recorder (ENG), and meanwhile examine serotonin (5-hydroxytryptamine, 5-HT) level in the MVN with microdialysis technique after caloric stimulation. Results In the caloric test the hot water (44℃) irrigation of the right external auditory canal induced horizontal nystagmus towards the right side lasting about 60 seconds and the ice water irrigation of the right external auditory canal induced it towards the left side lasting for about 90 seconds. No nystagmus was induced by 37℃ water irrigation of the external ear canal. Therefore, it was used as a negative control stimulation to the middle ear. The MVN 5-HT levels significantly increased in the first 5-minute collecting interval and increased to 254% and 189% of the control group in the second collecting interval in response to caloric vestibular stimulation with ice water and hot water respectively. The serotonin release was not distinctly changed by the irrigation of the auricle with ice water

  20. Spermatogenic cycle length and sperm production in a feral pig species (collared peccary, Tayassu tajacu).

    Science.gov (United States)

    Costa, Guilherme M J; Leal, Marcelo C; Silva, Jurupytan V; Ferreira, Ana Cássia S; Guimarães, Diva A; França, Luiz Renato de

    2010-01-01

    Although the collared peccary (Tayassu tajacu) is found throughout the Americas, with a high potential for domestication and commercial exploitation, there are few data on the reproductive biology of this mammalian species. The aim of the present study was to investigate testis structure, spermatogenic cycle length, Sertoli cell efficiency, and spermatogenic efficiency. Twelve adult peccaries were used for biometrical, histological, and stereological analyses; 3 of these peccaries received intratesticular injections of (3)H-thymidine for the determination of the duration of spermatogenesis. Testis weight and gonadosomatic index were 23.7 +/- 1.8 g and 0.2% +/- 0.1%, respectively. Seminiferous tubule volume density was 77.4% +/- 1.7%. Leydig cells occupied 12.8% +/- 1.8% of the testis parenchyma and presented a peculiar cytoarchitecture in the periphery of the seminiferous tubule lobes. The premeiotic, meiotic, and postmeiotic stage frequencies were very similar to those found for wild and domestic boars. The spermatogenic cycle and entire spermatogenic process (based on 4.5 cycles) lasted approximately 12.3 +/- 0.2 and 55.1 +/- 0.7 days, respectively. Daily sperm production per gram of testis in the collared peccary was approximately 23.4 +/- 2 x 10(6), which is similar to that of domestic and wild boars. The knowledge generated in the present study could be used in reproduction and animal improvement programs and provides important information that may be used for comparative reproductive biology with previously investigated mammalian species.

  1. Growth of and valine production by a Bacillus subtilis mutant in the small intestine of pigs

    DEFF Research Database (Denmark)

    Canibe, Nuria; Poulsen, Henrik Vestergaard; Nørgaard, Jan Værum;

    2016-01-01

    :Lys of 0.63:1 (Neg), 2) the Neg diet with added Bacillus subtilis-valine (1.28 × 108 cfu/g feed) (+Bac), and 3) the Neg diet with added L-Val to a Val:Lys of 0.69:1 (+Val). Eighteen gilts (6 on each treatment) with initial weights of ∼15 kg were fed the diets for 23 d before the animals were euthanized...... and samples from the small intestine were obtained. The number of B. subtilis cfu in digesta was higher in the +Bac group than in the Neg group (P cfu were detected in the Neg group, whereas numbers between 3.4 and 4.4 log cfu/g and numerically higher Val and Lys...... concentrations were measured in the +Bac group. Short-term in vitro incubations of digesta showed a decrease (P ≤ 0.03) in the number of B. subtilis cfu over time for the +Bac group and no difference in the rate of Val production compared to that in the Neg group. In conclusion, more B. subtilis cfu were present...

  2. Effects of amoxicillin, ceftiofur, doxycycline, tiamulin and tulathromycin on pig humoral immune responses induced by erysipelas vaccination.

    Science.gov (United States)

    Pomorska-Mól, M; Kwit, K; Wierzchosławski, K; Dors, A; Pejsak, Z

    2016-05-28

    It addition to their antimicrobial properties, antibiotics can influence the host immune system (modulation of cytokine secretion, antibody production and T-cell proliferation). In the present study, the authors studied the effects of therapeutic doses of amoxicillin (AMX), ceftiofur (CEF), doxycycline (DOXY), tiamulin (TIAM) and tulathromycin (TUL) on the postvaccinal immune response after pigs had been vaccinated against erysipelas. Because humoral immunity is considered as the most important in the protection against swine erysipelas, the present study focused on the interactions between antibiotics and postvaccinal humoral immunity. One hundred and five, eight-week-old pigs of both sexes were used. Specific antibodies to the Erysipelothrix rhusiopathiae antigen were determined using a commercial ELISA test. In pigs treated with DOXY or CEF or TIAM, a significant reduction in the number of positive pigs was observed four and six weeks after the second dose of vaccine, compared with the remaining vaccinated groups. In pigs treated with CEF, the ELISA score was significantly lower than in non-treated vaccinated pigs. While in vaccinated pigs treated with AMX or TUL, the ELISA score was significantly higher than in pigs treated with the remaining antibiotics and than in non-treated vaccinated controls. The results of the present study indicate that vaccination of pigs against erysipelas in the presence of antibiotics may result in a decrease (CEF, DOXY, TIAM) or enhancement (AMX, TUL) in the production of specific antibodies. British Veterinary Association.

  3. Impact of the amount of straw provided to pigs kept in intensive production conditions on the occurrence and severity of gastric ulceration at slaughter

    DEFF Research Database (Denmark)

    Herskin, Mette S.; Jensen, Henrik E.; Jespersen, Anna;

    2016-01-01

    as ‘permanent access’. The proportion of pigs with ulcerations was reduced by permanent access to straw (7 vs. 33%; P gastric ulceration in pigs.......This study examined effects of the amount of straw offered on occurrence and severity of gastric lesions in pigs kept in pens (18 pigs, 0.7 m2/pig) with partly slatted flooring and 10, 500 or 1000 g straw/pig/day from 30 kg live weight. The pigs had ad libitum access to dry feed. Forty-five pigs...... were used, three from each of 15 pens. After euthanization, the dimension of the non-glandular region of the stomach was measured. Lesions were characterized and scored. Irrespective of straw provided, 67% of the pigs showed signs of gastric pathology. Pigs provided with 500 or 1000 g straw were pooled...

  4. Impact of the amount of straw provided to pigs kept in intensive production conditions on the occurrence and severity of gastric ulceration at slaughter.

    Science.gov (United States)

    Herskin, Mette S; Jensen, Henrik E; Jespersen, Anna; Forkman, Björn; Jensen, Margit B; Canibe, Nuria; Pedersen, Lene J

    2016-02-01

    This study examined effects of the amount of straw offered on occurrence and severity of gastric lesions in pigs kept in pens (18 pigs, 0.7 m(2)/pig) with partly slatted flooring and 10, 500 or 1000 g straw/pig/day from 30 kg live weight. The pigs had ad libitum access to dry feed. Forty-five pigs were used, three from each of 15 pens. After euthanization, the dimension of the non-glandular region of the stomach was measured. Lesions were characterized and scored. Irrespective of straw provided, 67% of the pigs showed signs of gastric pathology. Pigs provided with 500 or 1000 g straw were pooled as 'permanent access'. The proportion of pigs with ulcerations was reduced by permanent access to straw (7 vs. 33%; Pstraw may improve animal health, and be considered as one possible strategy to limit gastric ulceration in pigs.

  5. THE EFFECT OF HOUSING SYSTEM ON THE INCIDENCE OF INTESTINAL PARASITE INFESTATION IN PIGS

    Directory of Open Access Journals (Sweden)

    Justyna BARTOSIK

    2012-12-01

    Full Text Available The aim of the study was to compare the intestinal parasite fauna occurring in pigs kept in different rearing conditions (group A – extremely extensive, B – extensive and C – intensive. In the period between March and July 2010, 345 samples of faeces from piglets (125, weaners (60, fatteners (94 and sows (66 were collected and tested, using standard coproscopic methods. Six parasitic species, belonging to phylum Nematoda (Strongyloides ransomi, Ascaris suum, Oesophagostomum dentatum, Trichuris suum and to phylum Apicomplexa (Isospora suis and Eimeria debliecki were diagnosed. Eggs of Toxascaris leonina, a parasitic roundworm mostly affecting the members of the Canidae and Felidae families, not encountered in pigs until now, were found in the faeces of fatteners. The highest level of parasite invasion was recorded in pigs from group A and B; they were multi-species invasions. Parasitic species found in faeces not only bring economic losses, but they may also be dangerous for human health and life, which indicates the necessity to implement parasitological screening protocols, especially in the extensive rearing conditions.

  6. Length of productive life of sows in four pig farms in the tropics of Mexico.

    Science.gov (United States)

    Segura-Correa, José C; Ek-Mex, Enrique J; Alzina-López, Alejandro; Magaña-Monforte, Juan G; Sarmiento-Franco, Luis; Santos-Ricalde, Ronald H

    2011-08-01

    The objective of this study was to determine the effect of some factors on the length of productive life (LPL), the number of parities (NP), and the culling interval (CI) of sows. The information from 2003 to 2009 of 3,746 sows with LPL and NP records and 5,819 sows with CI records from the four farms of Yucatan, Mexico was used. LPL was defined as the number of days between the first farrowing and culling or death and CI as the number of days between the last farrowing and culling. To determine the effects of farm, litter size at first farrowing (LSF1) and age at first farrowing (AF1) on LPL and NP, and of farm, LSF1 and culling reason on CI general linear models were used. Means and standard errors for LPL, NP, and CI were 496.4 ± 8.42 days, 3.35 ± 0.04 parities, and 48.3 ± 0.52 days, respectively. All factors had significant effects (P farm 2 stayed longer (806.0 days) and had more parities (5.25) than the sows from the other farms. Sows with the smallest litters (≤8 piglets) at first farrowing stayed the least time in the farm (675.1 days) and had the fewest parities (4.49). Gilts farrowing the first time before 331 days of age stayed longer time in the farm (726.4 days) and had more parities (4.81) in comparison with 331-348 and more than 348 days age groups. With respect to CI, the sows in farm 3 stayed the longest time in the farm. Sows with more than five parities were culled sooner after their last farrowing. In conclusion, differences between farms for LPL, PN, and CI were found. Females that farrowed at a short age and those with large litters stayed longer in the herd. Old sows and those culled for reproductive reasons stayed the least time in the herd after their last farrowing.

  7. Do the ban on use of anti-microbial growth promoter impact on technical change and the efficiency of slaughter-pig production

    DEFF Research Database (Denmark)

    Lawson, Lartey; Otto, Lars; Jensen, Peter Vig

    2005-01-01

    This study aims at investigating the effects of the ban on the use of anti-microbial growth promoters in the production of “Finishing Pigs” for slaughter. We investigate if the ban on the use of anti-microbial growth promoters has for specialised pig-producers altered the productivity of inputs......, technical change and the efficiency of production. This paper complements an earlier paper that investigated the impact of the ban on weaned-pig produc-tion. Background: The study is motivated by the fact that antimicrobial growth promoters have been known world wide to protect livestock from bacteria...... of the use of growth promoters in 1995, which re-sulted in a total ban in 2000. EU as a follow-up to the Danish action also initiated a ban on the use of growth promoters that are known to contribute to human health hazard. In the US the discussion of a ban is an ongoing subject of debate. For US producers...

  8. Development of molecular tools to differentiate Indian wild pig (Sus scrofa cristatus) meat from exotic and local domestic pig meat

    OpenAIRE

    Kajal Kumar Jadav; Avadh Bihari Shrivastav; Nidhi Rajput

    2013-01-01

    Aim: Identification of wild pig and domestic pig is essential to prevent illegal poaching of wild pig and to implement Wildlife (Protection) Act, 1972. PCR-RFLP was used to differentiate Wild pig (Sus scrofa cristatus) from Domestic pig (Sus scrofa domestica) meat. Materials and Methods: DNA was isolated from meat samples of both the sub species and a fragment of Cytochrome b gene was amplified using universal primers and the PCR products were subjected to restriction digestion. Results: All ...

  9. Brief Introduction to Compulsory Product Certification System

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ What is Compulsory Product Certification System? Compulsory Product Certification System is a kind of product qualifying assessment system which implements under laws and regulations by states in order to protect life security of human, animals and plants as well as environmental protection and national security.

  10. Sustainable livestock production: Low emission farm – The innovative combination of nutrient, emission and waste management with special emphasis on Chinese pig production

    Directory of Open Access Journals (Sweden)

    Thomas Kaufmann

    2015-09-01

    Full Text Available Global livestock production is going to be more and more sophisticated in order to improve efficiency needed to supply the rising demand for animal protein of a growing, more urban and affluent population. To cope with the rising public importance of sustainability is a big challenge for all animal farmers and more industrialized operations especially. Confined animal farming operations (CAFO are seen very critical by many consumers with regard to their sustainability performance, however, the need to improve the sustainability performance especially in the ecological and social dimension exists at both ends of the intensity, i.e., also for the small holder and family owned animal farming models. As in livestock operations, feed and manure contribute the majority to the three most critical environmental impact categories global warming potential (GWP, acidification (AP and eutrophication potential (EP any effort for improvement should start there. Intelligent combination of nutrient-, emission- and waste management in an integrated low emission farm (LEF concept not only significantly reduces the environmental footprint in the ecological dimension of sustainability, but by producing renewable energy (heat, electricity, biomethane with animal manure as major feedstock in an anaerobic digester also the economic dimension can be improved. Model calculations using new software show the ecological improvement potential of low protein diets using more supplemented amino acids for the Chinese pig production. The ecological impact of producing biogas or upgraded biomethane, of further treatment of the digestate and producing defined fertilizers is discussed. Finally, the LEF concept allows the integration of an insect protein plant module which offers additional ecological and economical sustainability improvement potential in the future. Active stakeholder communication about implementation steps of LEF examples improves also the social aspect of

  11. Evaluation of the RapID NH system for identification of Haemophilus somnus, Pasteurella multocida, Pasteurella haemolytica, and Actinobacillus pleuropneumoniae isolated from cattle and pigs with respiratory disease.

    OpenAIRE

    Salmon, S A; Watts, J L; Yancey, R J

    1993-01-01

    Haemophilus somnus, Pasteurella haemolytica, Pasteurella multocida, and Actinobacillus pleuropneumoniae from cattle and pigs with respiratory disease were used to evaluate the RapID NH system (Innovative Diagnostics, Atlanta, Ga.). Minor modifications of the RapID NH system to include animal source and growth requirements would permit the identification of all isolates tested.

  12. Energy savings in heating systems for nursery pigs; Uso racional de energia eletrica em sistema de aquecimento para creches de leitoes

    Energy Technology Data Exchange (ETDEWEB)

    Sarubbi, Juliana; Rossi, Luiz Antonio; Moura, Daniella Jorge de; Oliveira, Rafael Augustus de; David, Eduardo [Universidade Estadual de Campinas (FEAGRI/UNICAMP), SP (Brazil). Fac. de de Engenharia Agricola], Emails: rossi@feagri.unicamp.br, daniella.moura@feagri.unicamp.br, augustus@feagri.unicamp.br

    2009-07-01

    Energy saving procedures in pig industry are important to bring sustainability to this economic activity. Energy savings can contribute to the economic and environmental feasibility of the livestock and improve animal welfare conditions. This research work aimed to perform a comparison among three heating technologies for nursery pigs related to the energy saving aspects. Three heating systems were studied: TR - suspended electrical resistors, TP - heated floor and TA - convection heating. The heated floor system was considered better than other under the aspect of electrical energy use. (author)

  13. Pigs taking wing with transposons and recombinases

    Science.gov (United States)

    Clark, Karl J; Carlson, Daniel F; Fahrenkrug, Scott C

    2007-01-01

    Swine production has been an important part of our lives since the late Mesolithic or early Neolithic periods, and ranks number one in world meat production. Pig production also contributes to high-value-added medical markets in the form of pharmaceuticals, heart valves, and surgical materials. Genetic engineering, including the addition of exogenous genetic material or manipulation of the endogenous genome, holds great promise for changing pig phenotypes for agricultural and medical applications. Although the first transgenic pigs were described in 1985, poor survival of manipulated embryos; inefficiencies in the integration, transmission, and expression of transgenes; and expensive husbandry costs have impeded the widespread application of pig genetic engineering. Sequencing of the pig genome and advances in reproductive technologies have rejuvenated efforts to apply transgenesis to swine. Pigs provide a compelling new resource for the directed production of pharmaceutical proteins and the provision of cells, vascular grafts, and organs for xenotransplantation. Additionally, given remarkable similarities in the physiology and size of people and pigs, swine will increasingly provide large animal models of human disease where rodent models are insufficient. We review the challenges facing pig transgenesis and discuss the utility of transposases and recombinases for enhancing the success and sophistication of pig genetic engineering. 'The paradise of my fancy is one where pigs have wings.' (GK Chesterton). PMID:18047690

  14. Passive immunisation, an old idea revisited: Basic principles and application to modern animal production systems

    DEFF Research Database (Denmark)

    Hedegaard, Chris Juul; Heegaard, Peter M. H.

    2016-01-01

    remains big. This review highlights a number of examples on the use of passive immunisation for the control of infectious disease in the modern production of a range of animals, including pigs, cattle, sheep, goat, poultry and fish. Special emphasis is given on the enablement of passive immunisation...... strategies in these production systems through low cost and ease of use as well as on the sources, composition and purity of immunoglobulin preparations used and their benefits as compared to current measures, including vaccination (also comprising maternal vaccination), antibiotics and feed additives...... development of bacterial resistance, thus constituting a real and widely applicable alternative to antibiotics....

  15. The effect of ethanol or sorbitol on glucose production from pyruvate in isolated hepatocytes from 48-hour fasted guinea-pigs.

    Science.gov (United States)

    Armstrong, M K; Weissberger, L E

    1985-01-01

    Hepatocytes isolated from 48-hour, fasted guinea-pigs were incubated with glucose precursors to compare relative rates of glucose production. Glucose production from lactate and pyruvate was similar (2.61 vs 3.18 mumol/hr per 100 mg wet weight). Glucose production from fructose was greater than that from sorbitol (4.68 vs 1.63 mumol/hr per 100 mg wet weight). When ethanol was added to pyruvate-containing buffer, the flux of pyruvate to glucose and lactate was synergistically enhanced (5.28 vs 3.76 and 7.51 vs 2.88 mumol/hr per 100 mg wet weight, respectively). When sorbitol was added to buffer containing pyruvate, glucose and lactate production were even greater than that seen with ethanol (8.32 vs 5.38 and 15.99 vs 7.51 mumol/hr per 100 mg wet weight, respectively).

  16. Product service system product design task planning with supplier participation

    Directory of Open Access Journals (Sweden)

    Zhongqi Sheng

    2015-12-01

    Full Text Available Product service system is a new-type production system with high-degree integration and overall optimization of product and service. The participation of suppliers in product service system development will greatly shorten the development cycle and enhance the production efficiency. The task planning of product design is one of the key phases of product service system development process, which mainly contains the design task decomposition and the design task allocation. Aiming at product service system product design, this article conducts the decomposition of design tasks, performs the recognition of coupling design task set, and realizes the structured modeling of design tasks. Outsourcing decision-making analysis is conducted on the design tasks obtained through decomposition process so as to confirm the design tasks that need the participation of suppliers. The allocation model of design tasks is constructed, and the suppliers that take part in product service system development are confirmed based on genetic algorithm. Taking the numerical control machine tool as an example, this article conducts the instance analysis and verifies the feasibility of the forenamed methods.

  17. Product Configuration Systems - Implications for Product Innovation and Development

    DEFF Research Database (Denmark)

    Edwards, Kasper; Pedersen, Jørgen Lindgaard

    2004-01-01

    configurations. However, costs are but one parameter on which firms compete and firms must continually innovate new and develop existing products. This paper presents original empirical insights on implementation and use of product configuration systems in a number of Danish industrial firms. The paper discusses...... the organisational changes associated with PCS and how this affects product innovation and development. The paper begins by introducing product configuration systems, which are then placed in context to the firm as a process technology which coordinate different processes: product development, order acquisition......Product Configuration Systems (PCS) is a step in the direction of mass customization in the sense that PCS allows a firm to significantly lower the unit cost of configuration. Thus PCS is a valuable technology for lowering operating costs while retaining a high number of possible product...

  18. A dynamic growth model for prediction of nutrient partitioning and manure production in growing–finishing pigs: Model development and evaluation

    DEFF Research Database (Denmark)

    Danfær, Allan Christian; Jørgensen, Henry; Kebreab, E;

    2015-01-01

    minor mean bias. Root mean square prediction error (RMSPE) was used in evaluation of the model for its predictive power. The overall RMSPE was 2.2 and 4.1 g/d for protein and lipid deposition, respectively. The excretion database used for evaluation of the model was constructed from 150 digestibility...... trials using growing–finishing pig diets that had a wide range of nutrient chemical composition. Nutrient and water excretion were quantified using the principle of mass conservation. The average daily observed and predicted manure production was 3.79 and 3.99 kg/d, respectively, with a RMSPE of 0.49 kg...

  19. Consumer choice and suggested price for pork as influenced by its appearance, taste and information concerning country of origin and organic pig production

    DEFF Research Database (Denmark)

    Dransfield, E.; Ngapo, T.M.; Nielsen, Niels Asger

    2005-01-01

    Reactions of consumers to the appearance and taste of pork with and without information concerning outdoor production of pigs were tested in France, Denmark, Sweden and UK. Consumers in all four countries focussed on colour and fatness rather than marbling and drip to make their choice. Almost half...... of the British and Danish preferred the paler and the French the darker pork. Most people preferred the leaner pork. When information was provided in the form of labels, the vast majority of consumers preferred the pork labelled as originating from their own country as opposed to 'imported' and that labelled...

  20. Pesticide Product Information System (PPIS)

    Science.gov (United States)

    PPIS includes registrant name and address, chemical ingredients, toxicity category, product names, distributor brand names, site/pest uses, pesticidal type, formulation code, and registration status for all products registered in the U.S.

  1. Stimulation of butyrate production by gluconic acid in batch culture of pig cecal digesta and identification of butyrate-producing bacteria.

    Science.gov (United States)

    Tsukahara, Takamitsu; Koyama, Hironari; Okada, Masaaki; Ushida, Kazunari

    2002-08-01

    Gluconic acid reaches the large intestine to stimulate lactic acid bacteria. However, the fermentation pattern of gluconic acid has yet to be elucidated. Accordingly, we examined the fermentation properties induced by gluconic acid in the pig cecal digesta in vitro. We also tested sorbitol and glucose, substrates for which the fermentation rate and patterns are known. The gluconic acid-utilizing bacteria were further isolated from pig cecal digesta and identified to examine the effect of gluconic acid on hind gut fermentation. Gluconic acid was fermented more slowly than were the other two substrates. Gluconic acid stimulated butyrate production; the butyrate molar percentage reached 26%, which is considered a high butyrate production. The majority of gluconic acid fermenters were identified as lactic acid bacteria, such as Lactobacillus reuteri and L. mucosae, and acid-utilizing bacteria, such as Megasphaera elsdenii and Mitsuokella multiacida. The gluconic acid fermented by lactic acid bacteria, and the lactate and acetate that were produced were used to form butyrate by acid-utilizing bacteria, such as M. elsdenii. Gluconic acid may be useful as a prebiotic to stimulate butyrate production in the large intestine.

  2. A hierarchical Markov decision process modeling feeding and marketing decisions of growing pigs

    DEFF Research Database (Denmark)

    Pourmoayed, Reza; Nielsen, Lars Relund; Kristensen, Anders Ringgaard

    2016-01-01

    Feeding is the most important cost in the production of growing pigs and has a direct impact on the marketing decisions, growth and the final quality of the meat. In this paper, we address the sequential decision problem of when to change the feed-mix within a finisher pig pen and when to pick pigs...... for marketing. We formulate a hierarchical Markov decision process with three levels representing the decision process. The model considers decisions related to feeding and marketing and finds the optimal decision given the current state of the pen. The state of the system is based on information from on...

  3. EPIDEMIOLOGICAL ASPECTS OF A FOCUS OF Tunga penetrans INFESTATION IN OUTDOOR PIG PRODUCTION ASPECTOS EPIDEMIOLÓGICOS DE UM FOCO DE TUNGÍASE (Tunga penetrans, SIPHONAPTERA EM UM SISTEMA INTENSIVO DE SUÍNOS CRIADOS AO AR LIVRE

    Directory of Open Access Journals (Sweden)

    Doralice Pedroso de Paiva

    2007-09-01

    Full Text Available

    The outdoors intensive-type pig production is characterized by maintaining swine during reproductive, pregnancy and nursing periods on pasture limited by wiring and/or electric fences. The aim of the present report was to describe the epidemiological aspects of an outbreak of Tunga penetrans in an outdoor pig production system. A total of six mature boars, 9 lactating sows, 13 pregnant sows, 9 mating sows, 20 suckling pigs and 34 weaned pigs were clinically examined. None of the boars were infested, while 50% of all sows examined had the parasite lesions, most frequently observed in the breast area, thus resulting in agalactia in lactating sows due to obstruction of the galactophorus channel, with subsequent involution of the mammary gland. In 90% of suckling piglets walking difficulty was observed, caused by the presence of the parasite in the hoofs, with 25% of these animals also showing hock lesions (farm number 1, n = 48. Similar lesions were observed in 100% of 40-47 days old weaned pigs (farm number 2, n = 24 and in 80% of 70 days old weaned pigs (farm number 3, n = 10, all of which had been transferred to finishing units in a confinement system. Therefore we conclude that Tunga penetrans can infest pigs reared or kept outdoors, causing agalactia in lactating sows, and walking disorders in suckling and weaned pigs.

    KEY-WORDS: Chiggers, agalactia in sows; outdoors pig production.

    O sistema intensivo de produção de suínos criados ao ar livre (SISCAL é caracterizado por manter os suínos em piquetes com cobertura vegetal, nas fases de reprodução, maternidade e creche, cercados com tela e/ou fios de arame eletrificado. Este trabalho teve por objetivo relatar

  4. Environmental Assessment of Ronozyme® P5000 CT Phytase as an Alternative to Inorganic Phosphate Supplementation to Pig Feed Used in Intensive Pig Production

    DEFF Research Database (Denmark)

    Nielsen, Per Henning; Wenzel, Henrik

    2007-01-01

    of agricultural land is given very large relative weight. Hundreds of enzyme products are commercially available on the market today, each with a range of different applications. There are several indications that enzymes like Ronozyme Phytase can play an important role in a transition to a more sustainable...... of avoided contributions to global warming, acidification, photochemical ozone formation and particularly nutrient enrichment and by significant energy savings and particularly phosphate savings. A single trade-off in terms of agricultural land use for enzyme production is small and unimportant unless use...

  5. Prevalence and antimicrobial resistance in Salmonella enterica isolated from broiler chickens, pigs and meat products in Thailand-Cambodia border provinces.

    Science.gov (United States)

    Trongjit, Suthathip; Angkititrakul, Sunpetch; Tuttle, R Emerson; Poungseree, Jiratchaya; Padungtod, Pawin; Chuanchuen, Rungtip

    2017-01-01

    This study aimed to examine the prevalence and antimicrobial resistance (AMR) of Salmonella isolates from broiler chickens, pigs and their associated meat products in the Thailand-Cambodia border provinces. A total of 941 samples were collected from pigs and broiler chickens at slaughter houses and from carcasses at local fresh markets in Sa Kaeo, Thailand (n = 554) and Banteay Meanchey, Cambodia (n = 387) in 2014 and 2015. From these samples, 345 Salmonella isolates were collected from Sa Keao (n = 145; 23%) and Banteay Meanchey (n = 200; 47%) and assayed for antimicrobial susceptibility, class 1 integrons and extended-spectrum β-lactamase (ESBL) genes. Serovars Typhimurium (29%) and Rissen (29%) were the most common serotypes found in Thai and Cambodian isolates, respectively. Multidrug resistance was detected in 34% and 52% of isolates from Sa Keao and Banteay Meanchey, respectively. The majority of the Thai isolates were resistant to ampicillin (72.4%), whereas most Cambodian isolates were resistant to sulfamethoxazole (71%). Eleven isolates from Sa Keao and 44 from Banteay Meanchey carried class 1 integrons comprising resistance gene cassettes. The most common gene cassette array was dfrA12-aadA2 (61.1%). Six isolates were ESBL producers. The β-lactamase genes found included blaTEM-1 , blaCTX-M-55 and blaCMY-2 . Some of these class 1 integrons and ESBL genes were located on conjugative plasmid. In conclusion, multidrug-resistant Salmonella are common in pigs, chickens and their products in the Thailand-Cambodia border provinces. Our findings indicate that class 1 integrons play a role in spread of AMR in the strains in this study. © 2017 The Societies and John Wiley & Sons Australia, Ltd.

  6. Antagonistic effect of chitin-degrading microfungi on thick-shelled pig helminth eggs

    OpenAIRE

    Katakam, Kiran Kumar; Thapa, Sundar; Meyling, Nicholai V.; Dalsgaard, Anders; Roepstorff, Allan; Thamsborg, Stig Milan

    2011-01-01

    The rising demand for pigs produced in organic farming systems, which require access to outdoor areas and associated increased risk of gastrointestinal nematode infections, is creating growing interest in alternative control strategies. Also development of anthelminthic resistance due to extensive use of anthelmintics is a concern in intensive pig production. Use of chitin-degrading microfungi is one of the options. Extensive research has been conducted on biological control of plant pathogen...

  7. Implementation of a validated HACCP system for the control of microbiological contamination of pig carcasses at a small abattoir.

    Science.gov (United States)

    Bryant, Jeffrey; Brereton, Donald A; Gill, Colin O

    2003-01-01

    To guide the implementation of a Hazard Analysis Critical Control Point (HACCP) system at a small abattoir, the microbiological conditions of pig carcasses at various stages of processing were assessed by enumerating total aerobes, coliforms, and Escherichia coli in samples collected from randomly selected sites on the carcasses. Those data indicated that carcasses were contaminated with bacteria mainly during dehairing and operations on the head. When carcasses were pasteurized after head removal, the numbers of total aerobes on dressed carcasses were reduced by about 1 order and the numbers of coliforms and E. coli were reduced by more than 2 orders of magnitude. Implementation of an HACCP system on the basis of the microbiological data gave cooled carcasses with mean numbers of total aerobes < 100/cm2, and mean numbers of coliforms and E. coli about 1/1000 cm2.

  8. 9 CFR 113.38 - Guinea pig safety test.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Guinea pig safety test. 113.38 Section 113.38 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF... Standard Procedures § 113.38 Guinea pig safety test. The guinea pig safety test provided in this section...

  9. Nuclear STAT3 translocation in guinea pig and rat brain endothelium during systemic challenge with lipopolysaccharide and interleukin-6.

    Science.gov (United States)

    Rummel, Christoph; Voss, Thilo; Matsumura, Kiyoshi; Korte, Stefan; Gerstberger, Rüdiger; Roth, Joachim; Hübschle, Thomas

    2005-10-10

    During systemic inflammation, cytokines are released by immune-competent cells into the circulation, which in turn signal the brain to mediate brain-controlled signs of illness. Cytokine-responsive brain cells can be mapped by histological analysis of cytokine-induced transcription factors or transcription factor-associated molecules revealing different cell phenotypes that respond to activation of the immune system. Critical sites mediating cytokine-dependent immuneffector functions can be divided into two groups, one group of responding cells situated along a tight blood-brain barrier (BBB), and a second cell group in structures with an open BBB, e.g., the sensory circumventricular organs (CVOs). Previous reports from our group suggest that activation of the signal transducer and activator of transcription factor 3 (STAT3) during lipopolysaccharide (LPS)-induced systemic inflammation is mediated by interleukin-6 (IL-6) and occurs in astrocytes of the rat CVOs. Here we show in the guinea pig a time-dependent marked LPS-induced STAT3 activation within astrocytes and endothelial cells of the CVOs, within astrocytes located in brain structures with a functional BBB and within the brain endothelium of the entire brain. In addition, systemic treatment of rats with either rat recombinant IL-6 or LPS induced STAT3 activation in brain endothelial cells in a similar way as observed in the guinea pig brain, stressing the involvement of IL-6 in this phenomenon in a more generalized way. The STAT3-activated brain cells are located in critical target structures mediating cytokine action during LPS-induced inflammation. STAT3-controlled transcriptional activation with yet unknown cell-specific functional consequences seems to be involved in this process. (c) 2005 Wiley-Liss, Inc.

  10. Generation of Pig Induced Pluripotent Stem Cells with a Drug-Inducible System

    Institute of Scientific and Technical Information of China (English)

    Zhao Wu; Jijun Chen; Jiangtao Ren; Lei Bao; Jing Liao; Chun Cui; Linjun Rao; Hui Li; Yijun Gu; Huiming Dai; Hui Zhu; Xiaokun Teng; Lu Cheng; Lei Xiao

    2009-01-01

    Domesticated ungulate pluripotent embryonic stem (ES) cell lines would be useful for generating precise gene-modified animals. To date, many efforts have been made to establish domesticated ungulate pluripotent ES cells from early embryos without success.Here, we report the generation of porcine-induced pluripotent stem (iPS) cells using drug-inducible expression of defined factors.We showed that porcine iPS cells expressed alkaline phosphatase, SSEA3, SSEA4, Tra-1-60, Tra-1-81, Oct3/4, Nanog, Sox2, Rex1 and CDH1. Pig iPS cells expressed high levels of telomerase activity and showed normal karyotypes. These cells could differentiate into cell types of all three germ layers in vitro and in teratomas. Our study reveals properties of porcine pluripotent stem cells that may facilitate the eventual establishment of porcine ES cells. Moreover, the porcine iPS cells produced may be directly useful for the generation of precise gene-modified pigs.

  11. Production System Design for Multiple Brands

    DEFF Research Database (Denmark)

    Karlsson, Christer; Christensen, Irene

    appliances, cars, and trucks but also other products. An important denominator for the production system design is flow structures. Important dependent factors come from brand distinction, architectural characteristics, technology levels, supplier competence, process choice, process flow patterns, lay...

  12. Modernizing the Regulatory System for Biotechnology Products

    Science.gov (United States)

    This Web page describes the continuing effort to modernize the federal regulatory system for biotechnology products as well as clarify various roles of EPA, FDA and USDA in evaluating new biotechnology products.

  13. The effects of feeding rice in substitution of corn and the degree of starch gelatinization of rice on the digestibility of dietary components and productive performance of young pigs.

    Science.gov (United States)

    Vicente, B; Valencia, D G; Pérez-Serrano, M; Lázaro, R; Mateos, G G

    2008-01-01

    A 28-d trial was conducted to evaluate the effect of the main cereal of the diet (corn or rice), heat processing (HP) of rice, and the degree of starch gelatinization (SG) of rice on apparent total tract digestibility (ATTD) of dietary components and productive performance of pigs weaned at 25 d of age. The experimental design was a completely randomized, with 4 treatments and 8 replicates per treatment (5 pigs penned together). Control pigs were fed a complex diet without growth promoters and based on milk products, fish meal, and 50% cooked and flaked corn, with a degree of SG of 84%. Experimental groups received the same complex diet as the control group, but the corn was substituted by rice with 3 different degrees of SG; 11% that corresponded to raw rice and 52 or 76% that corresponded to cooked rice processed under 2 different conditions. Pig growth was measured at 25, 39, and 53 d of age, and ATTD was determined at 29, 39, and 53 d of age. The ATTD of all dietary components except for N increased with age (P <0.01) and were greater for the rice than for the corn diet. Heat processing of rice improved ATTD of all dietary components at 29 d of age, but no beneficial effects were observed at 39 or 53 d of age. Modifying the conditions of HP to increase the degree of SG of rice from 52 to 76% and to reduce mean particle size from 480 to 405 mum did not result in further improvement of nutrient digestibility. From 25 to 53 d of age, pigs fed rice consumed more feed (678 vs. 618 g/d; P <0.05), grew faster (466 vs. 407 g/d; P <0.01), and tended to have greater G:F (0.685 vs. 0.662; P <0.10) than pigs fed corn. In fact, from 25 to 39 d of age, pigs fed rice consumed 23% more feed (P <0.01), grew 29% faster (P <0.01), and had 5% greater G:F (P <0.05) than pigs fed corn. Feeding rice improved performance of weanling pigs, and HP of rice under mild conditions enhanced diet digestibility and productive performance of pigs. Severe processing of rice increased the degree

  14. Classification of pig farms regarding environmental risk and internal use of pig manure

    OpenAIRE

    Emonet-Denand, Virginie; Porphyre, Vincent; Thi Hoa Ly, Nguyen; Hoang Bien, Dang; Paillat, Jean-Marie

    2006-01-01

    224p; This paper presents the practices of Vietnamese farmers in handling animal excreta, i.e., pig manure, and highlights the difference of farms in terms of their operation relative to waste management and utilization. The environmental risks associated with these practices, and the attempt to quantify the difference of pork production systems, are also explored. Finally, a typology constructed based on the difference of practices and the quantification of risks is also given.

  15. Mapping of neurons in the central nervous system of the guinea pig by use of antisera specific to the molluscan neuropeptide FMRFamide

    DEFF Research Database (Denmark)

    Triepel, J; Grimmelikhuijzen, C J

    1984-01-01

    Immunoreactive neurons were mapped in the central nervous system of colchicine-treated and untreated guinea pigs with the use of two antisera to the molluscan neuropeptide FMRFamide. These antisera were especially selected for their incapability to react with peptides of the pancreatic polypeptide...

  16. NG-nitro L-arginine methyl ester: systemic and pulmonary haemodynamics, tissue blood flow and arteriovenous shunting in the pig

    NARCIS (Netherlands)

    E. Marcel van Gelderen (E.); M.O. den Boer (Marinus); P.R. Saxena (Pramod Ranjan)

    1993-01-01

    textabstractThe effects of NG-nitro-Lrarginine methyl ester (L-NAME), an inhibitor of the endothelial nitric oxide (NO) biosynthesis, on systemic and pulmonary haemodynamics, and tissue as well as arteriovenous anastomotic blood flows were investigated in the anaesthetized pig, using simultaneous in

  17. Product/Service-System Development

    DEFF Research Database (Denmark)

    Tan, Adrian; McAloone, Timothy Charles; Gall, Catherine

    2007-01-01

    Product/service-systems’ (PSS) are innovation strategies where instead of focusing on the value of selling physical products, one focuses on the value of the utility of products and services throughout the product’s life period. This approach enables companies to provide customers with offerings...... that continuously deliver value and create a strong competitive advantage. PSS attempts to transcend the old industrial credo that value is embedded in products and the consideration of ‘cost-quality-time’ relates to the physical artefact. While there is increasing interest in PSS, limited research has been done...... in the design and development aspects of PSS. The objective of this paper is to explore how a global manufacturing company is developing an offering to their customers that is focused on the utility of their products throughout their life time. The paper describes the process of how a company is shifting from...

  18. Mudular Product Families and Assembly Systems

    DEFF Research Database (Denmark)

    Thyssen, Jesper

    of modular product families and the interacting assembly system.   The thesis reviews extant theory and provides various classifications and discussions guided by the overall theme of product modularity and assembly systems. The empirical system analysis, based upon a longitudinal single case study......, articulates a system model incorporating both structural and performance elements. The extensive and detailed case analysis provides the necessary insight into the specific variables associated with the complex configuration of modular products and assembly systems.   Based upon the system model a number...

  19. Effect of the inclusion of dry pasta by-products at different levels in the diet of typical Italian finishing heavy pigs: Performance, carcass characteristics, and ham quality.

    Science.gov (United States)

    Prandini, A; Sigolo, S; Moschini, M; Giuberti, G; Morlacchini, M

    2016-04-01

    The effect of pasta inclusion in finishing pig diets was evaluated on growth performance, carcass characteristics, and ham quality. Pigs (144) were assigned to 4 diets with different pasta levels: 0 (control, corn-based diet), 30, 60, or 80%. Pigs fed pasta had greater (linear, PPasta increased (quadratic, PPasta decreased (linear, Ppasta. Pasta could be considered as an ingredient in the diet for typical Italian finishing heavy pigs.

  20. Ensiled citrus pulp as a by-product feedstuff for finishing pigs: nutritional value and effects on intestinal microflora and carcass quality

    Energy Technology Data Exchange (ETDEWEB)

    Moset, V.; Piquer, O.; Cervera, C.; Fernández, J.; Hernández, P.; Cerisuelo, A.

    2015-07-01

    Forty pigs of 76.8 ± 4.2 kg body weight were fed four different diets varying in ensiled citrus pulp (ECP) inclusion level (0, 50, 100, and 150 g of ECP/kg of diet on dry matter base). The trial lasted 5 weeks. During the last week, faecal samples were obtained to calculate apparent nutrient digestibility; also volatile fatty acids (VFA) content in faeces was determined. The digestible energy and protein of ECP was estimated by regression analysis. During the trial faecal samples were collected to determine enterobacteria and lactobacilli counts. At slaughter, carcass characteristics were registered. The inclusion of ECP in the diets decreased energy digestibility but increased neutral and acid detergent fibre digestibility linearly (p<0.05). The estimated digestible energy and protein of ECP were lower than expected (7.0 MJ/kg dry matter (DM) and 33.8 g/kg DM, respectively). Total VFA production in faeces was not affected by the diet. Both enterobacteria and lactobacilli counts were lower (p<0.01) with than without ECP inclusion at the end of the study. Carcass yield decreased linearly (p<0.05) and backfat at gluteus medius increased quadratically (p<0.05) with the inclusion of ECP in the diet. The polyunsaturated fatty acid content of the internal subcutaneous fat layer decreased quadratically (p<0.05) with the inclusion of ECP. Thus, the nutritive value of ECP for finishing pigs is low and levels of 150g/kg can negatively affect carcass yield. However, including up to 100 g of ECP/kg in finishing pig diets did not affect nutrient digestibility, carcass yield and subcutaneous fat FA profile. Additionally, increasing ECP levels in diets decreases faecal enterobacteria and lactobacilli counts in faeces. (Author)

  1. Ensiled citrus pulp as a by-product feedstuff for finishing pigs: nutritional value and effects on intestinal microflora and carcass quality

    Directory of Open Access Journals (Sweden)

    Verónica Moset

    2015-09-01

    Full Text Available Forty pigs of 76.8 ± 4.2 kg body weight were fed four different diets varying in ensiled citrus pulp (ECP inclusion level (0, 50, 100, and 150 g of ECP/kg of diet on dry matter base. The trial lasted 5 weeks. During the last week, faecal samples were obtained to calculate apparent nutrient digestibility; also volatile fatty acids (VFA content in faeces was determined. The digestible energy and protein of ECP was estimated by regression analysis. During the trial faecal samples were collected to determine enterobacteria and lactobacilli counts. At slaughter, carcass characteristics were registered. The inclusion of ECP in the diets decreased energy digestibility but increased neutral and acid detergent fibre digestibility linearly (p<0.05. The estimated digestible energy and protein of ECP were lower than expected (7.0 MJ/kg dry matter (DM and 33.8 g/kg DM, respectively. Total VFA production in faeces was not affected by the diet. Both enterobacteria and lactobacilli counts were lower (p<0.01 with than without ECP inclusion at the end of the study. Carcass yield decreased linearly (p<0.05 and backfat at gluteus medius increased quadratically (p<0.05 with the inclusion of ECP in the diet. The polyunsaturated fatty acid content of the internal subcutaneous fat layer decreased quadratically (p<0.05 with the inclusion of ECP. Thus, the nutritive value of ECP for finishing pigs is low and levels of 150g/kg can negatively affect carcass yield. However, including up to 100 g of ECP/kg in finishing pig diets did not affect nutrient digestibility, carcass yield and subcutaneous fat FA profile. Additionally, increasing ECP levels in diets decreases faecal enterobacteria and lactobacilli counts in faeces.

  2. Prevalence and pattern of antimicrobial susceptibility in Escherichia coli isolated from pigs reared under antimicrobial-free and conventional production methods.

    Science.gov (United States)

    Bunner, Christine A; Norby, Bo; Bartlett, Paul C; Erskine, Ronald J; Downes, Frances P; Kaneene, John B

    2007-07-15

    To determine and compare levels and patterns of antimicrobial resistance among Escherichia coli isolated from pigs on farms that did not use antimicrobial agents versus pigs produced under conventional methods. Cross-sectional study. Sample Population-35 antimicrobial-free and 60 conventional swine farms. Farms were visited once, and fecal samples were collected from 15 finisher pigs if available. One E coli isolate from each sample was tested for susceptibility pattern to 14 antimicrobial agents by use of microbroth dilution. E coli isolates were recovered from 1,381 (97.1%) of 1,422 fecal samples. Herd size was significantly larger for conventional swine farms. Resistance to ceftriaxone, ciprofloxacin, or nalidixic acid was not observed on any of the 95 farms. Three isolates from 2 conventional farms were resistant to ceftiofur. Conventional farms had significantly higher levels of resistance to ampicillin, sulfamethoxazole, tetracycline, and chloramphenicol, compared with antimicrobial-free farms. Fourteen percent of E coli isolates were susceptible or had intermediate resistance to all the tested antimicrobial agents. The 3 most frequent patterns of multiple resistance were streptomycin-tetracycline, sulfamethoxazole-tetracycline, and kanamycin-streptomycin-sulfamethoxazole-tetracycline. Cessation of antimicrobial use did not appear to result in an immediate reduction in antimicrobial resistance in swine farms. Prospective studies of long-term antimicrobial usage and cessation are needed to estimate the extent to which food animal production may be contributing to antimicrobial drug resistance and might provide a direct measure of the rates of reversibility of antimicrobial drug resistance that might be achieved by curtailing antimicrobial usage.

  3. Production systems design : a product oriented approach and methodology

    OpenAIRE

    2008-01-01

    Production systems design is critical to achieving manufacturing performance and objectives. Although generic approaches to this design are available they may not be able to objectively address specific manufacturing configurations. Here it is argued that product oriented manufacturing (POM) organization offers advantages in relation to the function oriented one. Based on this, a methodology specially addressing POM systems design, and prototype of a Computer Aided Design Syste...

  4. Quantitative assessment of glial cells in the human and guinea pig enteric nervous system with an anti-Sox8/9/10 antibody.

    Science.gov (United States)

    Hoff, Sebastian; Zeller, Florian; von Weyhern, Claus Werner Hann; Wegner, Michael; Schemann, Michael; Michel, Klaus; Rühl, Anne

    2008-08-01

    Quantitative changes of enteric glia (EGC) have been implicated in gastrointestinal disorders. To facilitate future studies of EGC in human pathology, we aimed to characterize thoroughly glial markers in the human enteric nervous system (ENS) and to compare EGC in man and guinea pig. Whole-mount preparations of the enteric nerve plexuses from human and guinea pig ileum and colon were labeled with antibodies against S100b, glial fibrillary acidic protein (GFAP), and p75NGFR and the transcription factors Sox8/9/10 and neuronally counterstained. Abundant immunoreactivity (IR) for S100b, GFAP, p75NGFR, and Sox8/9/10 was detected in EGC of all studied regions. Although the cytoplasmatic staining pattern of most markers did not permit glial quantification, the nuclear localization of Sox8/9/10-IR allowed to identify and count all EGC individually. In both man and guinea pig, myenteric ganglia were larger and contained more EGC and neurons than submucous ganglia. Furthermore, there were more EGC in the human than in the guinea pig myenteric plexus (MP), glial density was consistently higher in the human ENS, and the glia index (glia:neuron ratio) ranged from 1.3 to 1.9 and from 5.9 to 7.0 in the human submucous plexus (SMP) and MP, respectively, whereas, in guinea pig, the glia index was 0.8-1.0 in the SMP and 1.7 in the MP. The glia index was the most robust quantitative descriptor within one species. This is a comprehensive set of quantitative EGC measures in man and guinea pig that provides a basis for pathological assessment of glial proliferation and/or degeneration in the diseased gut.

  5. Application of proton-transfer-reaction mass spectrometry to the assessment of odorant removal in a biological air cleaner for pig production.

    Science.gov (United States)

    Hansen, Michael J; Liu, Dezhao; Guldberg, Lise Bonne; Feilberg, Anders

    2012-03-14

    There is an urgent need to develop odor reduction technologies for animal production facilities, and this requires a reliable measurement technique for estimating the removal of odorants. The purpose of the present experiment was to investigate the application of proton-transfer-reaction mass spectrometry (PTR-MS) for continuous measurements at a biofilter from SKOV A/S installed at a pig production facility. PTR-MS was able to handle the harsh conditions with high humidity and dust load in a biofilter and provide reliable data for the removal of odorants, including the highly odorous sulfur compounds. The biofilter removed 80-99% of carboxylic acids, aldehydes, ketones, phenols, and indoles and ca. 75% of hydrogen sulfide. However, only ~0-15% of methanethiol and dimethyl sulfide was removed. In conclusion, PTR-MS is a promising tool that can be used to improve the development of biological air cleaning and other odor reduction technologies toward significant odorants.

  6. Robust Medical Isotope Production System

    Energy Technology Data Exchange (ETDEWEB)

    Klein, Steven Karl [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Kimpland, Robert Herbert [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-06-15

    The success of this theoretical undertaking provided confidence that the behavior of new and evolving designs of fissile solution systems may be accurately estimated. Scaled up versions of SUPO, subcritical acceleratordriven systems, and other evolutionary designs have been examined.

  7. Robust Medical Isotope Production System

    Energy Technology Data Exchange (ETDEWEB)

    Klein, Steven Karl [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Kimpland, Robert Herbert [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-06-15

    The success of this theoretical undertaking provided confidence that the behavior of new and evolving designs of fissile solution systems may be accurately estimated. Scaled up versions of SUPO, subcritical acceleratordriven systems, and other evolutionary designs have been examined.

  8. Product Configuration Systems and Consequences for Productivity and Quality

    DEFF Research Database (Denmark)

    Pedersen, Jørgen Lindgaard; Edwards, Kasper

    2004-01-01

    Product Configuration Systems (PCS) are systems to produce quotations to potential buyers of the products an enterprise produces. Typically the products are complex and will first be produced according to specific needs from the customers. From twelve case studies in Danish industry...... with informations about expected and realised costs and benefits in implemented PCS's we have the following results: the three highest aggregated scoring expected benefits are: 1)improved quality in specifications, 2)lower turnaround time, and 3)using less resources.The results achieved are in accordance...

  9. Methanol production method and system

    Science.gov (United States)

    Chen, Michael J.; Rathke, Jerome W.

    1984-01-01

    Ethanol is selectively produced from the reaction of methanol with carbon monoxide and hydrogen in the presence of a transition metal carbonyl catalyst. Methanol serves as a solvent and may be accompanied by a less volatile co-solvent. The solution includes the transition metal carbonyl catalysts and a basic metal salt such as an alkali metal or alkaline earth metal formate, carbonate or bicarbonate. A gas containing a high carbon monoxide to hydrogen ratio, as is present in a typical gasifer product, is contacted with the solution for the preferential production of ethanol with minimal water as a byproduct. Fractionation of the reaction solution provides substantially pure ethanol product and allows return of the catalysts for reuse.

  10. Production by R-alpha-methylhistamine of a histamine H3 receptor-mediated decrease in basal vascular resistance in guinea-pigs.

    Science.gov (United States)

    McLeod, R L; Gertner, S B; Hey, J A

    1993-10-01

    1. The effect of the selective histamine H3 receptor agonist, R-alpha-methylhistamine given intravenously (10-100 micrograms kg-1) was examined on baseline total peripheral resistance (TPR), and cardiovascular haemodynamics in bilaterally vagotomized, anaesthetized guinea-pigs. 2. R-alpha-methylhistamine produced a dose-dependent hypotension and fall in TPR at 30 and 100 micrograms kg-1. A decrease in heart rate (HR) was observed at a dose of 100 micrograms kg-1. R-alpha-methylhistamine (10-100 micrograms kg-1) also produced a dose-dependent fall in rate pressure product (RPP). There was no effect on cardiac output (CO) or stroke volume (SV) at these doses. 3. Histamine H1 and H2 blockade in animals pretreated with a combination of chlorpheniramine (0.3 mg kg-1) and cimetidine (3.0 mg kg-1) did not alter the haemodynamic actions of R-alpha-methyl-histamine (100 micrograms kg-1, i.v.). Pretreatment with the selective H3 antagonist, thioperamide (1 mg kg-1), completely blocked the action of R-alpha-methylhistamine on haemodynamic parameters. 4. To study the mechanism of action of R-alpha-methylhistamine, the vasodilator hydralazine (1 mg kg-1, i.v.) was used. Hydralazine lowered BP, TRP and RPP in guinea-pigs pretreated with ipratropium (50 micrograms kg-1, i.v.). Hydralazine had no effect on HR, SV or CO. 5. R-alpha-methylhistamine (100 micrograms kg-1) did not affect the vasopressor action and increases in TPR produced by adrenaline (1 and 3 micrograms kg-1). On the other hand, the vasodilator hydralazine (1 mg kg-1, i.v.) inhibited the effects of adrenaline (3 micrograms kg-1) on TPR and RPP. The effect of both doses of adrenaline on BP were attenuated by hydralazine. Therefore, the inhibitory effects of R-alpha-methylhistamine are not mediated through a direct action on vascular smooth muscle.6. In adrenalectomized guinea-pigs, R-alpha-methylhistamine (100 microg kg-1) produced a drop in BP and HR.There was no difference between the effects of R

  11. Mars Integrated Propellant Production System Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The Integrated Mars In-Situ Propellant Production System (IMISPPS) is an end-to-end system that will produce rocket propellant on Mars from CO2 in the Martian...

  12. Mars Integrated Propellant Production System Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The Integrated Mars In-Situ Propellant Production System (IMISPPS) is an end-to-end system that will produce rocket propellant on Mars from CO2 in the Martian...

  13. The beauty in product-service systems

    NARCIS (Netherlands)

    Post, R.A.G.; Da Silva Cardozo, O.; Hekkert, P.P.M.

    2015-01-01

    Aesthetic appreciation affects the success of products in a number of areas, such as user satisfaction and usability. Hence, designers apply aesthetic principles to create more successful products. However, it is still unclear how such principles apply to the services and systems that products are o

  14. Rapid cooling after acute hyperthermia alters intestinal tissue morphology and increases the systemic inflammatory response in pigs

    Science.gov (United States)

    Acute hyperthermia can result in mortality if recovery is not appropriately managed. The study objective was to determine the effects of heatstroke recovery methods on the physiological response in pigs. In four repetitions, 36 male pigs (88.7 ± 1.6 kg BW) were exposed to thermoneutral conditions (T...

  15. Nitrogen cycling in an ecological farming system of milk vetch culture - pig raising - biogas fermentation - rice culture

    NARCIS (Netherlands)

    Liu, J.R.; Berge, ten H.F.M.; Zhang, M.L.; Wu, J.F.; Guo, C.Z.; Liu, W.

    2002-01-01

    The [15]N - labeled technique was used to study the stock, transformation, fate and utilization efficiency of N in the farming-pig husbandry-biogas ecosystem in rice areas. It was shown that the crude protein digestibility of the ensilaged milk vetch by pig was 53.76%, the recovery rates of ensilage

  16. Assessing product service systems (PSS)

    DEFF Research Database (Denmark)

    Hsuan, Juliana; McAloone, Tim C.; Neugebauer, Line

    We apply a management tool to classify different integrated PSS within the context of Danish maritime industry. We investigate how a firm should organize its operations to manage the transition from being a manufacturing to a servitizing company, and to provide a guideline for strategic positioni...... and planning of the balance between product sales and service activities....

  17. System of Natural Health Products

    Directory of Open Access Journals (Sweden)

    Angelica Paoletti

    2011-01-01

    Full Text Available Introduction. The safety of vitamin K antagonists (VKA use can be compromised by many popular herbal supplements taken by individuals. The literature reports that 30% of warfarin-treated patients self-medicates with herbs. Possible interactions represent an health risk. We aimed to identify all herbs-oral anticoagulants interactions collected in the Italian database of suspected adverse reactions to “natural health” products. Methods. The Italian database of spontaneous reports of suspected adverse reactions to natural products was analyzed to address herb-VKAs interactions. Results. From 2002 to 2009, we identified 12 reports with 7 cases of INR reduction in patients treated with warfarin (n=3 and acenocoumarol (n=4, and 5 cases of INR increase (all warfarin associated. It was reported 8 different herbal products as possibly interacting. Discussion. Our study confirms the risk of interactions, highlighting the difficulty to characterize them and their mechanisms and, finally, prevent their onset. The reported data underline the urgent need of healthcare providers being aware of the possible interaction between natural products and VKA, also because of the critical clinical conditions affecting patients. This is the first step to have the best approach to understand possible INR alterations linked to herb-VKA interaction and to rightly educate patients in treatment with VKA.

  18. Research Progress on Inulin in Raising Pigs Production%菊粉在养猪生产中应用的研究进展

    Institute of Scientific and Technical Information of China (English)

    上官明军; 王芳; 张红岗; 张元庆; 张变英

    2013-01-01

    菊粉通常是从植物中提取的一种特殊的天然果聚糖类碳水化合物,具有益生作用,可选择性地促进肠道中有益菌增殖,抑制有害菌生长,提高动物生长性能和免疫功能.作者主要从菊粉作为一种新型饲料添加剂,对猪肠道菌群、生长性能、免疫功能、矿物质吸收利用和肉品质影响等方面进行综述.%Inulin is a kind of special natural fructan of carbohydrate, usually extracted from plants. It had a probiotic effect, and could selectively improve the beneficial intestinal microflora and inhibit enteric pathogens, furthermore. It could enhance production performance and immune function of animals. This article expounded the research progress on inulin in raising pigs as a new feed additive, and whose effect on the intestinal flora, the growth performance, the immune function, the mineral absorption and utilization, and meat quality of pigs and so on were analyzed in this paper.

  19. Genetic diversity, virulotyping and antimicrobial resistance susceptibility of Yersinia enterocolitica isolated from pigs and porcine products in Malaysia.

    Science.gov (United States)

    Thong, Kwai Lin; Tan, Lai Kuan; Ooi, Peck Toung

    2017-05-24

    The objectives of the present study were to determine the antimicrobial resistance, virulotypes and genetic diversity of Yersinia enterocolitica isolated from uncooked porcine food and live pigs in Malaysia. Thirty-two non-repeat Y. enterocolitica strains of three bioserotypes (3 variant/O:3, n = 27; 1B/O:8, n = 3; 1A/O:5, n = 2) were analysed. Approximately 90% of strains were multidrug-resistant with a multiple antibiotic resistance index Malaysia. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  20. Expected energy production evaluation for photovoltaic systems

    DEFF Research Database (Denmark)

    Ding, Yi; Østergaard, Jacob; Peng, Wang

    2011-01-01

    A photovoltaic (PV) system consists of many solar panels, which are connected in series, parallel or a combination of both. Energy production for the PV system with various configurations is different. In this paper, a methodology is developed to evaluate and analyze the expected energy production...