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Sample records for phytic acid

  1. Autohydrolysis of phytic acid.

    Science.gov (United States)

    Hull, S R; Gray, J S; Montgomery, R

    1999-09-10

    The autohydrolysis of phytic acid at 120 degrees C resulted in the formation of most of the phosphate esters of myo-inositol in varying amounts depending upon the reaction time. Eighteen of the 39 chromatographically distinct myo-inositol mono-, bis-, tris-, tetrakis-, pentakis-, and hexakisphosphates have been characterized using two different HPLC systems. These myo-inositol phosphates were partially purified by preparative anion-exchange chromatography under acidic and alkaline elution conditions. The combination of these two methods provides a two-tiered chromatographic approach to the rapid and sensitive identification of inositol phosphates in complex mixtures. Identification of the products was confirmed by 1D and 2D (1)H NMR analysis. The analytical procedure was applied to the autohydrolysis of the mixture of inositol phosphates from corn steep water.

  2. Phytic acid in green leaves.

    Science.gov (United States)

    Hadi Alkarawi, H; Zotz, G

    2014-07-01

    Phytic acid or phytate, the free-acid form of myo-inositolhexakiphosphate, is abundant in many seeds and fruits, where it represents the major storage form of phosphorus. Although also known from other plant tissues, available reports on the occurrence of phytic acid, e.g. in leaves, have never been compiled, nor have they been critically reviewed. We found 45 published studies with information on phytic acid content in leaves. Phytic acid was almost always detected when studies specifically tried to detect it, and accounted for up to 98% of total P. However, we argue that such extreme values, which rival findings from storage organs, are dubious and probably result from measurement errors. Excluding these high values from further quantitative analysis, foliar phytic acid-P averaged 2.3 mg·g(-1) , and represented, on average, 7.6% of total P. Remarkably, the ratio of phytic acid-P to total P did not increase with total P, we even detected a negative correlation of the two variables within one species, Manihot esculenta. This enigmatic finding warrants further attention.

  3. Agronomic Performance of Low Phytic Acid Wheat

    Science.gov (United States)

    Low phytic acid (LPA) genotypes of wheat are one approach to improving the nutritional quality of wheat by reducing the concentration of phytic acid in the aleurone layer, thus reducing the chelation of nutritionally important minerals and improving the bioavailability of phosphorus. Field studies ...

  4. NUTRITIONAL AND BAKING QUALITY OF LOW PHYTIC ACID WHEAT

    Science.gov (United States)

    Phytic acid is the major storage form of phosphorus in wheat grain. Non-ruminant animals cannot utilize phytic acid phosphorus, and phytic acid reduces the nutritional availability of important minerals. We have identified a wheat mutant (Lpa1-1) with reduced phytic acid P and increased inorganic ...

  5. Forward genetics studies of seed phytic acid

    Science.gov (United States)

    Both the chemical composition and total amount of seed phosphorus (P) are important to the end-use quality of cereal and legume seed crops. The chemistry of seed total P largely revolves around the synthesis and storage of phytic acid (myo-inositol hexaphosphate). Forward genetics research, beginnin...

  6. Study of thermal behavior of phytic acid

    Directory of Open Access Journals (Sweden)

    André Luis Máximo Daneluti

    2013-06-01

    Full Text Available Phytic acid is a natural compound widely used as depigmenting agent in galenic cosmetic emulsions. However, we have observed experimentally that phytic acid, when heated to 150 ºC for around one hour, shows evidence of thermal decomposition. Few studies investigating this substance alone with regard to its stability are available in the literature. This fact prompted the present study to characterize this species and its thermal behavior using thermal analysis (TG/DTG and DSC and to associate the results of these techniques with those obtained by elemental analysis (EA and absorption spectroscopy in the infrared region. The TG/DTG and DSC curves allowed evaluation of the thermal behavior of the sample of phytic acid and enabled use of the non-isothermal thermogravimetric method to study the kinetics of the three main mass-loss events: dehydration I, dehydration II and thermal decomposition. The combination of infrared absorption spectroscopy and elemental analysis techniques allowed evaluation of the intermediate products of the thermal decomposition of phytic acid. The infrared spectra of samples taken during the heating process revealed a reduction in the intensity of the absorption band related to O-H stretching as a result of the dehydration process. Furthermore, elemental analysis results showed an increase in the carbon content and a decrease in the hydrogen content at temperatures of 95, 150, 263 and 380 °C. Visually, darkening of the material was observed at 150 °C, indicating that the thermal decomposition of the material started at this temperature. At a temperature of 380 °C, thermal decomposition progressed, leading to a decrease in carbon and hydrogen. The results of thermogravimetry coupled with those of elemental analysis allow us to conclude that there was agreement between the percentages of phytic acid found in aqueous solution. The kinetic study by the non-isothermal thermogravimetric method showed that the dehydration

  7. Radioactively labelled phytic acid from maturing seeds of Sinapis alba

    Energy Technology Data Exchange (ETDEWEB)

    Blaicher, F.M.; Mukherjee, K.D.

    Maturing seeds of Sinapis alba were incubated with D-(U-/sup 14/C)glucose, sodium (1-/sup 14/C) acetate or myo-(U/sup 14/C) inositol in order to prepare radioactively labelled phytic acid with high specific activity. Although each substrate was utilized for the biosynthesis of phytic acid, maximum incorporation of radioactivity into phytic acid was found with myo-inositol. Radiochemical purity of the (U-/sup 14/C)phytic acid preparations was confirmed by chromatographic techniques. Such preparations should be useful for the study of interaction of phytic acid with metal ions and proteins and may serve as substrate in the assay should be useful for the study of interaction of phytic acid with metal ions and proteins and may serve as substrate in the assay of phytase.

  8. A modification of a method for phytic acid determination

    OpenAIRE

    DRAGUTIN VELICKOVIC; BILJANA VUCELIC-RADOVIC; SRDJAN BLAGOJEVIC; MIROLJUB BARAC; SLADJANA STANOJEVIC; MAJA LJUBICIC

    1999-01-01

    Several different methods for the determination of phytic acid which do not require sophisticated equipment are currently in use. These methods have been developed to measure the phytic acid content of a variety of materials, such as plant tissues, food stuffs and soils. The aim of the work was to modify the supernatant difference, macro-method developed by Erdman and Thompson1 for the determination of the phytic acid content in soybeans and soybean protein products. A micro-method was develo...

  9. Phytic Acid Inhibits Lipid Peroxidation In Vitro

    OpenAIRE

    Alicja Zajdel; Adam Wilczok; Ludmiła Węglarz; Zofia Dzierżewicz

    2013-01-01

    Phytic acid (PA) has been recognized as a potent antioxidant and inhibitor of iron-catalyzed hydroxyl radical formation under in vitro and in vivo conditions. Therefore, the aim of the present study was to investigate, with the use of HPLC/MS/MS, whether PA is capable of inhibiting linoleic acid autoxidation and Fe(II)/ascorbate-induced peroxidation, as well as Fe(II)/ascorbate-induced lipid peroxidation in human colonic epithelial cells. PA at 100 μM and 500 μM effectively inhibited the deca...

  10. Low Phytic Acid Barley Responses to Phosphorus Rates

    Science.gov (United States)

    Low phytic acid (LPA) barley (Hordeum vulgare L.) cultivars partition phosphorus in seed tissue differently than conventional barley cultivars through a reduction in seed phytic acid (myo-inositol-1,2,3,4,5,6-hexkisphosphate) coupled with an increase in inorganic phosphorus. The response of the LPA...

  11. Effect of phytic acid on suicidal erythrocyte death.

    Science.gov (United States)

    Eberhard, Matthias; Föller, Michael; Lang, Florian

    2010-02-10

    Phytic acid, an anticarcinogenic food component, stimulates apoptosis of tumor cells. Similar to apoptosis, human erythrocytes may undergo suicidal death or eryptosis, characterized by cell membrane scrambling and cell shrinkage. Triggers of eryptosis include energy depletion. Phytate intake could cause anemia, an effect attributed to iron complexation. The present experiments explored whether phytic acid influences eryptosis. Supernatant hemoglobin concentration was determined to reveal hemolysis, annexin V-binding in FACS analysis was utilized to identify erythrocytes with scrambled cell membrane, forward scatter in FACS analysis was taken as a measure of cell volume, and a luciferin-luciferase assay was employed to determine erythrocyte ATP content. As a result, phytic acid (>or=1 mM) did not lead to significant hemolysis, but significantly increased the percentage of annexin V-binding erythrocytes, significantly decreased forward scatter, and significantly decreased cellular ATP content. In conclusion, phytic acid stimulates suicidal human erythrocyte death, an effect paralleling its proapoptotic effect on nucleated cells.

  12. Phytic Acid Inhibits Lipid Peroxidation In Vitro

    Directory of Open Access Journals (Sweden)

    Alicja Zajdel

    2013-01-01

    Full Text Available Phytic acid (PA has been recognized as a potent antioxidant and inhibitor of iron-catalyzed hydroxyl radical formation under in vitro and in vivo conditions. Therefore, the aim of the present study was to investigate, with the use of HPLC/MS/MS, whether PA is capable of inhibiting linoleic acid autoxidation and Fe(II/ascorbate-induced peroxidation, as well as Fe(II/ascorbate-induced lipid peroxidation in human colonic epithelial cells. PA at 100 μM and 500 μM effectively inhibited the decay of linoleic acid, both in the absence and presence of Fe(II/ascorbate. The observed inhibitory effect of PA on Fe(II/ascorbate-induced lipid peroxidation was lower (10–20% compared to that of autoxidation. PA did not change linoleic acid hydroperoxides concentration levels after 24 hours of Fe(II/ascorbate-induced peroxidation. In the absence of Fe(II/ascorbate, PA at 100 μM and 500 μM significantly suppressed decomposition of linoleic acid hydroperoxides. Moreover, PA at the tested nontoxic concentrations (100 μM and 500 μM significantly decreased 4-hydroxyalkenal levels in Caco-2 cells which structurally and functionally resemble the small intestinal epithelium. It is concluded that PA inhibits linoleic acid oxidation and reduces the formation of 4-hydroxyalkenals. Acting as an antioxidant it may help to prevent intestinal diseases induced by oxygen radicals and lipid peroxidation products.

  13. Phytic acid inhibits lipid peroxidation in vitro.

    Science.gov (United States)

    Zajdel, Alicja; Wilczok, Adam; Węglarz, Ludmiła; Dzierżewicz, Zofia

    2013-01-01

    Phytic acid (PA) has been recognized as a potent antioxidant and inhibitor of iron-catalyzed hydroxyl radical formation under in vitro and in vivo conditions. Therefore, the aim of the present study was to investigate, with the use of HPLC/MS/MS, whether PA is capable of inhibiting linoleic acid autoxidation and Fe(II)/ascorbate-induced peroxidation, as well as Fe(II)/ascorbate-induced lipid peroxidation in human colonic epithelial cells. PA at 100 μM and 500 μM effectively inhibited the decay of linoleic acid, both in the absence and presence of Fe(II)/ascorbate. The observed inhibitory effect of PA on Fe(II)/ascorbate-induced lipid peroxidation was lower (10-20%) compared to that of autoxidation. PA did not change linoleic acid hydroperoxides concentration levels after 24 hours of Fe(II)/ascorbate-induced peroxidation. In the absence of Fe(II)/ascorbate, PA at 100 μM and 500 μM significantly suppressed decomposition of linoleic acid hydroperoxides. Moreover, PA at the tested nontoxic concentrations (100 μM and 500 μM) significantly decreased 4-hydroxyalkenal levels in Caco-2 cells which structurally and functionally resemble the small intestinal epithelium. It is concluded that PA inhibits linoleic acid oxidation and reduces the formation of 4-hydroxyalkenals. Acting as an antioxidant it may help to prevent intestinal diseases induced by oxygen radicals and lipid peroxidation products.

  14. Low phytic acid, low stachyose, high sucrose soybean lines

    OpenAIRE

    2008-01-01

    The present invention provides novel soybean lines having high sucrose content and low phytic acid and low stachyose content. The soybeans are easily digested and provide high energy content for animals and humans. The low phytic acid content permits animal feed to be produced that does not require phytase, yet does not result in significant production of pollution to the environment from excretion by farm animals.

  15. Phytic acid increases mucin and endogenous amino acid losses from the gastrointestinal tract of chickens.

    Science.gov (United States)

    Onyango, Edward M; Asem, Elikplimi K; Adeola, Olayiwola

    2009-03-01

    The influence of the form of phytic acid on the regulation of mucin and endogenous losses of amino acids, nitrogen and energy in chickens was investigated. Forty-eight 10-week-old male broilers were grouped by weight into eight blocks of six cages with one bird per cage. Birds received by intubation six dextrose-based combinations of phytic acid and phytase arranged in a 3 x 2 factorial consisting of phytic acid form (no phytic acid, 1.0 g free phytic acid or 1.3 g magnesium-potassium phytate) and phytase (0 or 1000 units). Each bird received the assigned combination added to 25 g dextrose at each of the two feedings on the first day of experimentation. All excreta were collected continuously for 54 h following feeding and frozen until analysed. Frozen excreta were thawed, pooled for each bird, lyophilised, ground, and analysed for DM, energy, nitrogen, amino acids, mucin, and sialic and uric acids. Chickens fed either magnesium-potassium phytate or free phytic acid showed increased (P phytate than with free phytic acid treatment. Both phytic acid treatments also increased (P < 0.05) endogenous loss of threonine, proline and serine. In conclusion, the form of phytic acid fed to chickens affects the extent of mucin and endogenous amino acid losses from the gastrointestinal tract.

  16. Phytic acid reduction in soy protein improves zinc bioavailability

    Energy Technology Data Exchange (ETDEWEB)

    Zhou, J.R.; Wong, M.S.; Burns, R.A.; Erdman, J.W. Jr. (Univ. of Illinois, Urbana (United States) Mead Johnson Research Center, Evansville, IN (United States))

    1991-03-15

    The objective of this study was to confirm previous studies that have suggested that reduction of phytic acid in soy improved zinc bioavailability (BV). Two commercially-produced soybean isolates containing either a normal phytic acid level or a reduced level were formulated into diets so as to provide 6 or 9 ppm zinc. Control diets were egg white protein-based and contained 3, 6 or 9 ppm zinc from zinc carbonate. Weanling male rats were fed these diets for 21 days and food intake and weight gain monitored. Slope ratio analysis of total tibia zinc content compared to total zinc intake revealed that zinc BV from reduced phytic acid soy isolate-containing diets was indistinguishable from control egg white diets. In contrast, zinc BV from normal soy isolate diets was significantly reduced compared to reduced phytic acid and control diets. These results coupled with other results indicate that phytic acid is the inhibitory factor in soybean products that results in reduced zinc BV.

  17. Metagenomic Analysis of the Rhizosphere Soil Microbiome with Respect to Phytic Acid Utilization

    OpenAIRE

    Unno, Yusuke; Shinano, Takuro

    2012-01-01

    While phytic acid is a major form of organic phosphate in many soils, plant utilization of phytic acid is normally limited; however, culture trials of Lotus japonicus using experimental field soil that had been managed without phosphate fertilizer for over 90 years showed significant usage of phytic acid applied to soil for growth and flowering and differences in the degree of growth, even in the same culture pot. To understand the key metabolic processes involved in soil phytic acid utilizat...

  18. Identification of novel rice low phytic acid mutations via TILLING by sequencing

    Science.gov (United States)

    Phytic acid (myo-inositol-1,2,3,4,5,6-hexakisphosphate or InsP6) accounts for 75-85% of the total phosphorus in seeds. Low phytic acid (lpa) mutants exhibit decreases in seed InsP6 with corresponding increases in inorganic P which, unlike phytic acid P, is readily utilized by humans and monogastric ...

  19. Inositol metabolism and phytase activity in normal and low phytic acid soybean seed

    Science.gov (United States)

    The genetic basis for the low seed phytic acid trait in soybean lines derived from the low phytic acid line (CX1834) of Wilcox et al (2000) is under investigation in several laboratories. Our objective was to measure metabolite levels associated with the phytic acid and raffinosaccharide biosyntheti...

  20. Influence of phytic acid concentration on performance of phytic acid conversion coatings on the AZ91D magnesium alloy

    Energy Technology Data Exchange (ETDEWEB)

    Cui Xiufang [School of Materials Science and Chemical Engineering, Harbin Engineering University, 145 Nantong Street, Harbin 150001 (China); State Key Laboratory for Corrosion and Protection, Institute of Metal Research, Chinese Academy of Sciences, 62 Wencui Road, Shenyang 110016 (China)], E-mail: cuixf97721@yahoo.com.cn; Li Ying [State Key Laboratory for Corrosion and Protection, Institute of Metal Research, Chinese Academy of Sciences, 62 Wencui Road, Shenyang 110016 (China); Li Qingfen; Jin Guo; Ding Minghui [School of Materials Science and Chemical Engineering, Harbin Engineering University, 145 Nantong Street, Harbin 150001 (China); Wang Fuhui [School of Materials Science and Chemical Engineering, Harbin Engineering University, 145 Nantong Street, Harbin 150001 (China); State Key Laboratory for Corrosion and Protection, Institute of Metal Research, Chinese Academy of Sciences, 62 Wencui Road, Shenyang 110016 (China)

    2008-10-15

    In this study, the phytic acid conversion coating, a new environmentally friendly chemical protective coating for magnesium alloys, was prepared. The influences of phytic acid concentration on the formation process, microstructure, chemical state and corrosion resistance of the conversion coatings on AZ91D magnesium alloy were investigated by means of weight gain measurement, field-emission scanning electron microscopy (FESEM), Fourier transform infrared (FTIR) spectroscopy, potentiodynamic polarization method and electrochemical impedance spectroscopy (EIS), respectively. And the depth profile of all elements in the optimal conversion coatings was analyzed by auger electron spectroscopy (AES). The results show that the growth, microstructure, chemical state and corrosion resistance of the conversion coatings are all obviously affected by the phytic acid concentration. The concentration of 5 g l{sup -1} corresponds to the maximum weight gain. The main elements of the coating are Mg, Al, O, P, and C, which are distributed gradually in depth. The functional groups of conversion coatings formed in higher concentration phytic acid solution are closer to the constituent of phytic acid than those formed in lower concentration phytic acid solution. The coatings formed in 1-5 g l{sup -1} are integrated and uniform. However, those formed in 20-50 g l{sup -1} have some micro-cracks on the {alpha} phase. The coating formed in 5 g l{sup -1} has the best corrosion resistance, whose open circuit current density decreases about six orders than that of the untreated sample, although the coatings deposited in 1-20 g l{sup -1} can all improve the corrosion resistance of AZ91D.

  1. Screening of soybean germplasm for high inorganic phosphorus and low phytic acid

    Directory of Open Access Journals (Sweden)

    S. Abirami, A. Kalamani and T. Kalaimagal

    2014-09-01

    Full Text Available Phytic acid, is the major storage form of phosphorus in soybean [Glycine max (L. Merr.] which comprises 75% of total seed phosphorus. It decreases the availability of some essential elements via bonding between the negatively charged phytic acid and the positively charged elements. Thus, diets high in phytate may lead to nutrient deficiencies. So, identification of lines with low phytic acid is of paramount importance. A germplasm survey was conducted among 250 soybean accessions to identify the accessions with low phytic acid. Phytic acid content ranged between 0.84 and 7.07 mg/ g of soy flour. The genotypes with low phytic acid content viz., Williams, Williams 82, CNS – AVRDC line, RKS 52 and NRC 66 may be useful in breeding programs for the development of low phytic acid genotypes with improved nutritional value to overcome nutritional deficiency syndromes and meet the demand of biofortification.

  2. Low phytic acid lentils (Lens culinaris L.): a potential solution for increased micronutrient bioavailability.

    Science.gov (United States)

    Thavarajah, Pushparajah; Thavarajah, Dil; Vandenberg, Albert

    2009-10-14

    Phytic acid is an antinutrient present mainly in seeds of grain crops such as legumes and cereals. It has the potential to bind mineral micronutrients in food and reduce their bioavailability. This study analyzed the phytic acid concentration in seeds of 19 lentil ( Lens culinaris L.) genotypes grown at two locations for two years in Saskatchewan, Canada. The objectives of this study were to determine (1) the levels of phytic acid in commercial lentil genotypes and (2) the impact of postharvest processing and (3) the effect of boiling on the stability of phytic aid in selected lentil genotypes. The phytic acid was analyzed by high-performance anion exchange separation followed by conductivity detection. The Saskatchewan-grown lentils were naturally low in phytic acid (phytic acid = 2.5-4.4 mg g(-1); phytic acid phosphorus = 0.7-1.2 mg g(-1)), with concentrations lower than those reported for low phytic acid mutants of corn, wheat, common bean, and soybean. Decortication prior to cooking further reduced total phytic acid by >50%. As lowering phytic acid intake can lead to increased mineral bioavailability, dietary inclusion of Canadian lentils may have significant benefits in regions with widespread micronutrient malnutrition.

  3. A modified colorimetric method for phytic acid analysis in soybean

    Science.gov (United States)

    A quantitative, reproducible, and efficient phytic acid assay procedure is needed to screen breeding populations and support genetic studies in soybeans. The objective of this study was to modify the colorimetric Wade reagent method and compare the accuracy and applicability of this new method in de...

  4. The Low Phytic Acid Phenotype in Soybean Line CX1834 is Due to Mutations in Two Homologues of the Maize Low Phytic Acid Gene

    Science.gov (United States)

    Plant seeds accumulate phosphorus in the form of myo-inositol-1,2,3,4,5,6 hexa-kisphosphate, commonly referred to as phytic acid. Phytic acid is found complexed with cationic mineral species in the form of phytate, which is not well digested or absorbed by monogastric species such as humans, poultry...

  5. Faecal phytic acid and its relation to other putative markers of risk for colorectal cancer.

    OpenAIRE

    Owen, R W; Weisgerber, U M; Spiegelhalder, B; Bartsch, H

    1996-01-01

    AIMS--Phytic acid, a major constituent of cereals, pulses, and seeds has been advocated as an important antioxidant component of dietary fibre that affords possible protection against colorectal cancer. This is supported by experimental studies showing it has antineoplastic activity in animal models of both colon and breast cancer. To date the concentration of faecal phytic acid in human clinical groups has not been evaluated. Therefore the faecal phytic acid content of adenoma patients drawn...

  6. The Potential Benefits and Adverse Effects of Phytic Acid Supplement in Streptozotocin-Induced Diabetic Rats

    OpenAIRE

    Omoruyi, F. O.; Budiaman, A.; Eng, Y.; F. E. Olumese; Hoesel, J. L.; Ejilemele, A.; Okorodudu, A. O.

    2013-01-01

    In this study, the effect of phytic acid supplement on streptozotocin-induced diabetic rats was investigated. Diabetic rats were fed rodent chow with or without phytic acid supplementation for thirty days. Blood and organ samples were collected for assays. The average food intake was the highest and the body weight gain was the lowest in the group fed phytic acid supplement compared to the diabetic and normal control groups. There was a downward trend in intestinal amylase activity in the gro...

  7. Screening of soybean germplasm for high inorganic phosphorus and low phytic acid

    OpenAIRE

    S. Abirami, A. Kalamani and T. Kalaimagal

    2014-01-01

    Phytic acid, is the major storage form of phosphorus in soybean [Glycine max (L.) Merr.] which comprises 75% of total seed phosphorus. It decreases the availability of some essential elements via bonding between the negatively charged phytic acid and the positively charged elements. Thus, diets high in phytate may lead to nutrient deficiencies. So, identification of lines with low phytic acid is of paramount importance. A germplasm survey was conducted among 250 soybean accessions to identify...

  8. Tethered phytic acid as a probe for measuring phytase activity.

    Science.gov (United States)

    Berry, Duane F; Berry, David A

    2005-06-15

    A novel approach for measuring phytase activity is presented. We have developed a new chromophoric substrate analog of phytic acid, 5-O-[6-(benzoylamino)hexyl]-d-myo-inositol-1,2,3,4,6-pentakisphosphate that permits direct measurement of the phosphate ester bond-cleavage reaction using HPLC. This compound, along with its dephosphorylated T-phosphatidylinositol intermediates, are quantified using reversed phase chromatography with UV detection.

  9. Metabolite profiling of two low phytic acid (lpa) rice mutants.

    Science.gov (United States)

    Frank, Thomas; Meuleye, Bertrand Seumo; Miller, Andreas; Shu, Qing-Yao; Engel, Karl-Heinz

    2007-12-26

    Two low phytic acid (lpa) rice mutant lines, Os-lpa-XS110-1 and Os-lpa-XS110-2, were grown together with their parent wild-type variety Xiushui 110 in four field trials. HPLC analysis of inositol phosphates in the seeds produced demonstrated that compared to the wild-type, the reduction in phytic acid content in Os-lpa-XS110-1 (-46%) was more pronounced than that in Os-lpa-XS110-2 (-23%). Lower inositol phosphates (InsP 3, InsP 4, InsP 5) were not detected in the mutants. The lpa mutants and the wild-type rice were subjected to comparative metabolite profiling by capillary gas chromatography. On average, 34% (Os-lpa-XS110-1) and 42% (Os-lpa-XS110-2) of the detected peaks were statistically significantly different between wild-type and mutants. However, only a few of these differences could be consistently observed for all field trials. Identification and quantification of the consistently different metabolites revealed that contents of myo-inositol and raffinose were increased in Os-lpa-XS110-1 but decreased in Os-lpa-XS110-2 compared to the wild-type. In addition, Os-lpa-XS110-1 exhibited increased levels of galactose and galactinol. Consideration of these metabolic changes in light of the routes involved in the biosynthesis of phytic acid indicated a disturbance in the early biosynthetic pathway of phytic acid in Os-lpa-XS110-2 (similar to the lpa-1 type mutation in maize) and a mutation event affecting phosphorylation of myo-inositol in Os-lpa-XS110-1 (similar to the lpa-3-type mutation).

  10. Metagenomic analysis of the rhizosphere soil microbiome with respect to phytic acid utilization.

    Science.gov (United States)

    Unno, Yusuke; Shinano, Takuro

    2013-01-01

    While phytic acid is a major form of organic phosphate in many soils, plant utilization of phytic acid is normally limited; however, culture trials of Lotus japonicus using experimental field soil that had been managed without phosphate fertilizer for over 90 years showed significant usage of phytic acid applied to soil for growth and flowering and differences in the degree of growth, even in the same culture pot. To understand the key metabolic processes involved in soil phytic acid utilization, we analyzed rhizosphere soil microbial communities using molecular ecological approaches. Although molecular fingerprint analysis revealed changes in the rhizosphere soil microbial communities from bulk soil microbial community, no clear relationship between the microbiome composition and flowering status that might be related to phytic acid utilization of L. japonicus could be determined. However, metagenomic analysis revealed changes in the relative abundance of the classes Bacteroidetes, Betaproteobacteria, Chlorobi, Dehalococcoidetes and Methanobacteria, which include strains that potentially promote plant growth and phytic acid utilization, and some gene clusters relating to phytic acid utilization, such as alkaline phosphatase and citrate synthase, with the phytic acid utilization status of the plant. This study highlights phylogenetic and metabolic features of the microbial community of the L. japonicus rhizosphere and provides a basic understanding of how rhizosphere microbial communities affect the phytic acid status in soil.

  11. Effect of Phytic Acid on the Texture of Tofu and the Precipitaion Reaction in Tofu Making

    OpenAIRE

    Setyono, Agus

    2014-01-01

    Soybean curd or tofu is widely used as daily foods by people in Indonesia. One of the delicate steps in the preparation of tofu is the addition of a salt to precipitate soy protein. The common salt of calcium sulfate and the peeled soybeans were used to produced the non phytic acid soybean milk. Futhermore the adjustment of phytic acid content in soybean milk was done by addition of phytic acid. The result showed that phytic acid is the most important factor in the formation of precipitated p...

  12. Reduction of phytic acid in soybean products improves zinc bioavailability in rats.

    Science.gov (United States)

    Zhou, J R; Fordyce, E J; Raboy, V; Dickinson, D B; Wong, M S; Burns, R A; Erdman, J W

    1992-12-01

    The inhibitory effect of phytic acid in soybean products on zinc bioavailability was evaluated in two experiments in rats. In Experiment 1, soybean flours containing different natural phytic acid levels produced by sand culture techniques that limited phosphorus during growth of the soybean plants were formulated into diets. The rats fed a higher phytic acid level diet had lower food intake, depressed weight gain, and lower tibia zinc gain (P phytic acid level was found. In Experiment 2, two commercially produced soybean isolates containing either normal phytic acid level or a reduced level were formulated into diets. Slope ratio analysis revealed that relative zinc bioavailability from phytic acid-containing soybean isolate-based diets was significantly reduced (P phytic acid soybean isolate-containing diets resulted in a significant increase of zinc bioavailability compared with normal phytic acid diets (P phytic acid is the primary inhibitory factor in soybean products that results in reduced zinc bioavailability and that phytate reduction in soybean protein increases zinc bioavailability.

  13. The effects of phytic acid on the Maillard reaction and the formation of acrylamide.

    Science.gov (United States)

    Wang, Huan; Zhou, Yamin; Ma, Jimei; Zhou, Yuanyuan; Jiang, Hong

    2013-11-01

    Phytic acid, myo-inositol hexaphosphoric acid, exists in substantial (1-5%) amounts in edible plant seeds. In this study the effects of phytic acid on the Maillard reaction and the formation of acrylamide were investigated. Both phytic acid and phosphate enhanced browning in glucose/β-alanine system, but phytic acid was less effective than phosphate. Higher pH favoured the catalytic activities for both of them. The influence of the types of sugar and amino acid on the reaction was also examined. Browning was suppressed by the addition of calcium and magnesium ions, but an additive effect was observed for ferrous ions and phytic acid in glucose/β-alanine solution at pH 8.0. Both phytic acid and phosphate promoted the polymerisation of the reaction intermediates. The kinetics of Maillard reaction was first-ordered reaction in the presence of phytic acid. Phytic acid was less effective than phosphate in the formation of acrylamide. When potato slices were treated with sodium phytate and calcium chloride successively, the formation of acrylamide was greatly suppressed.

  14. Effects of phytic acid on peanut allergens and allergenic properties of extracts.

    Science.gov (United States)

    Chung, Si-Yin; Champagne, Elaine T

    2007-10-31

    Phytic acid would form soluble and insoluble complexes with proteins. Our objective was to determine if phytic acid forms insoluble complexes with major peanut allergens, and if such reaction results in a peanut extract with a lower level of soluble allergens and allergenic property. Extracts from raw and roasted peanuts were treated with and without phytic acid at various pH values and then analyzed by SDS-PAGE and a competitive inhibition ELISA (ciELISA). The ciELISA measured IgE binding using a pooled serum from peanut-allergic individuals. Results showed that phytic acid formed complexes with the major peanut allergens (Ara h 1 and Ara h 2), which were insoluble in acidic and neutral conditions. Succinylation of the allergens inhibited complex formation, indicating that lysine residues were involved. A 6-fold reduction in IgE binding or allergenic potency of the extract was observed after treatment with phytic acid. It was concluded that phytic acid formed insoluble complexes with the major peanut allergens, and resulted in a peanut extract with reduced allergenic potency. Application of phytic acid to a peanut butter slurry presented a similar result, indicating that phytic acid may find use in the development of hypoallergenic peanut-based products.

  15. Feeding low-phytic acid corn grain to finishing wethers does not alter phosphorus digestion

    Science.gov (United States)

    Low-phytic acid (LPA) feed grains contain similar concentrations of P as do standard grains, but the majority of P exists as inorganic phosphate rather than phytic acid. Research has shown that LPA feeds can be used to improve overall efficiency of P utilization in swine, poultry and aquaculture pro...

  16. The rice OsLpa1 gene encodse a novel protein involved in phytic acid metabolism

    Science.gov (United States)

    The rice low phytic acid 1 (OsLpa1) gene was originally identified using a forward genetics approach. Mutation of this gene resulted in a 45% reduction in rice seed phytic acid with a molar-equivalent increase in inorganic phosphorus; however, the rice lpa1 mutant does not appear to differ significa...

  17. The potential benefits and adverse effects of phytic Acid supplement in streptozotocin-induced diabetic rats.

    Science.gov (United States)

    Omoruyi, F O; Budiaman, A; Eng, Y; Olumese, F E; Hoesel, J L; Ejilemele, A; Okorodudu, A O

    2013-01-01

    In this study, the effect of phytic acid supplement on streptozotocin-induced diabetic rats was investigated. Diabetic rats were fed rodent chow with or without phytic acid supplementation for thirty days. Blood and organ samples were collected for assays. The average food intake was the highest and the body weight gain was the lowest in the group fed phytic acid supplement compared to the diabetic and normal control groups. There was a downward trend in intestinal amylase activity in the group fed phytic acid supplement compared to the other groups. The spike in random blood glucose was the lowest in the same group. We noted reduced serum triglycerides and increased total cholesterol and HDL cholesterol levels in the group fed phytic acid supplement. Serum alkaline phosphatase and alanine amino transferase activities were significantly (P phytic acid supplementation. Systemic IL-1 β level was significantly (P phytic acid supplementation may be beneficial in the management of diabetes mellitus. The observed adverse effect on the liver may be due to the combined effect of streptozotocin-induced diabetes and phytic acid supplementation.

  18. Development of PCR-Based DNA markers flanking three low phytic acid mutant loci in barley

    Science.gov (United States)

    Phytic acid (PA) is the most abundant form of phosphorus (P) in cereal grains. PA chelates mineral cations to form an indigestible salt, and is thus regarded as an antinutritional agent and a contributor to water pollution. Grain with low phytic acid (lpa) genotypes could aid in mitigating this prob...

  19. Phytic acid protects porcine intestinal epithelial cells from deoxynivalenol (DON) cytotoxicity.

    Science.gov (United States)

    Pacheco, Graziela Drociunas; Silva, Caio Abércio da; Pinton, Philippe; Oswald, Isabelle P; Bracarense, Ana Paula Frederico Rodrigues Loureiro

    2012-05-01

    The purpose of this study was to evaluate the effects of phytic acid (IP(6)) as a possible inhibitor of cellular damage induced by toxic substances such as mycotoxins on a porcine intestinal epithelial cell line (IPEC-1). We first observed that a dose of 5 mM phytic acid decreases cell viability and transepithelial electrical resistance (TEER) of cell monolayer. We next investigate the effect of non-cytotoxic dose of phytic acid on the deoxinivalenol (DON) induced decreased TEER. We showed that treatment with 0.5 mM or 1.0 mM phytic acid restores the decrease in TEER caused by 25 μM DON. In conclusion this study demonstrates that phytic acid decreased the negative effects of deoxynivalenol on the membrane integrity of the IPEC-1 intestinal epithelial cell line.

  20. Recurrent selection to alter grain phytic acid concentration and iron bioavailability

    Science.gov (United States)

    Most of the phosphorus (P) in cereal grains is in the form of phytic acid, a potent inhibitor of iron absorption that cannot be digested by monogastric livestock or humans. High phytate content in staple crops contributes to the high incidence of iron deficiency in developing countries. Low phytic a...

  1. Effect of phytic acid, ethylenediaminetetraacetic acid, and chitosan solutions on microhardness of the human radicular dentin

    Directory of Open Access Journals (Sweden)

    Vineeta Nikhil

    2016-01-01

    Full Text Available Aim: The purpose of this study was to evaluate the effect of phytic acid, ethylenediaminetetraacetic acid (EDTA, and chitosan solutions on the microhardness of human radicular dentin. Materials and Methods: Thirty dentin specimens were randomly divided into three groups of 10 specimens each according to the irrigant used: G1 - 1% phytic acid, G2 - 17% EDTA, and G3 - 0.2% chitosan. A standardized volume of each chelating solution was used for 3 min. Dentin microhardness was measured before and after application at the cervical, middle, and apical levels with a Vickers indenter under a 200-g load and a 10-s dwell time. The results were analyzed using one-way analysis of variance (ANOVA and Student′s t test. Results: Microhardness of the radicular dentin varied at the cervical, middle, and apical levels. EDTA had the greatest overall effect, causing a sharp percentage reduction in dentin microhardness with a significant difference from phytic acid and chitosan (P = 0.002. However, phytic acid and chitosan differed insignificantly from each other (P = 0.887. Conclusion: All tested chelating solutions reduced microhardness of the radicular dentin layer at all the levels. However, reduction was least at the apical level. EDTA caused more reduction in dentin microhardness than chitosan while phytic acid reduced the least.

  2. Drug-likeness of Phytic Acid and Its Analogues.

    Science.gov (United States)

    Joy, Amitha; Balaji, S

    2015-01-01

    Inositol hexakisphosphate is known to be the phosphorous reserve in plants particularly in the seeds. Though it has been known for its antinutrient properties for many years, recent research shed light to reveal it as a novel anticancer agent. Hence the present study investigates the drug-likeness of phytic acid and its analogues through bioinformatics methods. Two potential cancer drug targets such as mitogen activated kinase and inositol 1,4,5-triphosphate receptor are included in the study. Out of 50 selected analogues of phytic acid, 42 structures interact well with the chosen drug targets. The best interacting structures are 1-diphosinositol pentakisphosphate and 2,3,4,5,6-pentaphosphonooxycyclohexyl dihydrogen phosphate. For both of these structures, the negative binding energy obtained was -49.5 KJ/mol; this affirms the stability of the complex. ADME properties are also predicted to assess the drug-like properties of the compounds. The structure activity relationship model is generated for 12 compounds with experimental IC50 values.

  3. Protection of 2024-T3 aluminium alloy by corrosion resistant phytic acid conversion coating

    Science.gov (United States)

    Shi, Hongwei; Han, En-Hou; Liu, Fuchun; Kallip, Silvar

    2013-09-01

    The corrosion protection properties of environmentally friendly phytic acid conversion coatings were studied on 2024-T3 aluminium alloy. The films were prepared under acidic conditions with various pH values and characterised by SEM, EDS, ATR-FTIR and electrochemical techniques. The results indicate that the conversion coatings obtained by immersing the alloy in phytic acid solutions at pH from 3 to 5.5 provide excellent corrosion resistance. ATR-FTIR confirms that the film is formed by deposition of reaction products between Al3+ and phosphate groups in phytic acid molecules. The conformation models of the deposition film are proposed.

  4. Effect of phytic acid on the microstructure and corrosion resistance of Ni coating

    Energy Technology Data Exchange (ETDEWEB)

    Meng Guozhe, E-mail: mengguozhe@hrbeu.edu.c [Corrosion and Protection Laboratory, Key Laboratory of Superlight Materials and Surface Technology, Harbin Engineering University, Ministry of Education, Harbin 150001 (China)] [State Key Laboratory for Corrosion and Protection, Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016 (China); Sun Feilong [Corrosion and Protection Laboratory, Key Laboratory of Superlight Materials and Surface Technology, Harbin Engineering University, Ministry of Education, Harbin 150001 (China); Shaoa Yawei [State Key Laboratory for Corrosion and Protection, Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016 (China); Zhang Tao; Wang Fuhui [Corrosion and Protection Laboratory, Key Laboratory of Superlight Materials and Surface Technology, Harbin Engineering University, Ministry of Education, Harbin 150001 (China)] [State Key Laboratory for Corrosion and Protection, Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016 (China); Dong Chaofang [Corrosion and Protection Center, University of Science and Technology Beijing, Beijing 100083 (China); Li, Xiaogang [State Key Laboratory for Corrosion and Protection, Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016 (China)] [Corrosion and Protection Center, University of Science and Technology Beijing, Beijing 100083 (China)

    2010-08-01

    In this work, the pure Ni coatings were synthesized on Q235 steel by using reverse pulsed electrodeposition technique in sulphate-based baths with 0, 0.1, 0.2 and 0.3 g/L phytic acid additive. The effect of phytic acid on the microstructure and micro-morphology of the sample was observed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM), respectively. And the effect of phytic acid on the corrosion resistance of the sample was studied by potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) techniques. The results demonstrated that the addition of phytic acid was in favor of the growth of nano-scale twins (NT) in the interior of grains, which was due to the lowered stacking fault energies of Ni during the electrodeposition, and the typical morphology of pyramidal islands on the surface. The results also demonstrated that the effect of phytic acid was not monotonous with increasing concentration: the passive current density i{sub p} was minimum and the charge transfer resistance R{sub t} was maximum for the sample obtained from the bath with 0.2 g/L phytic acid, indicating that the sample obtained from the bath with 0.2 g/L phytic acid showed the best corrosion resistance.

  5. Overexpression of a Gene Involved in Phytic Acid Biosynthesis Substantially Increases Phytic Acid and Total Phosphorus in Rice Seeds.

    Science.gov (United States)

    Tagashira, Yusuke; Shimizu, Tomoe; Miyamoto, Masanobu; Nishida, Sho; Yoshida, Kaoru T

    2015-04-24

    The manipulation of seed phosphorus is important for seedling growth and environmental P sustainability in agriculture. The mechanism of regulating P content in seed, however, is poorly understood. To study regulation of total P, we focused on phytic acid (inositol hexakisphosphate; InsP₆) biosynthesis-related genes, as InsP₆ is a major storage form of P in seeds. The rice (Oryza sativa L.) low phytic acid mutant lpa1-1 has been identified as a homolog of archael 2-phosphoglycerate kinase. The homolog might act as an inositol monophosphate kinase, which catalyzes a key step in InsP₆ biosynthesis. Overexpression of the homolog in transgenic rice resulted in a significant increase in total P content in seed, due to increases in InsP₆ and inorganic phosphates. On the other hand, overexpression of genes that catalyze the first and last steps of InsP₆ biosynthesis could not increase total P levels. From the experiments using developing seeds, it is suggested that the activation of InsP₆ biosynthesis in both very early and very late periods of seed development increases the influx of P from vegetative organs into seeds. This is the first report from a study attempting to elevate the P levels of seed through a transgenic approach.

  6. Overexpression of a Gene Involved in Phytic Acid Biosynthesis Substantially Increases Phytic Acid and Total Phosphorus in Rice Seeds

    Directory of Open Access Journals (Sweden)

    Yusuke Tagashira

    2015-04-01

    Full Text Available The manipulation of seed phosphorus is important for seedling growth and environmental P sustainability in agriculture. The mechanism of regulating P content in seed, however, is poorly understood. To study regulation of total P, we focused on phytic acid (inositol hexakisphosphate; InsP6 biosynthesis-related genes, as InsP6 is a major storage form of P in seeds. The rice (Oryza sativa L. low phytic acid mutant lpa1-1 has been identified as a homolog of archael 2-phosphoglycerate kinase. The homolog might act as an inositol monophosphate kinase, which catalyzes a key step in InsP6 biosynthesis. Overexpression of the homolog in transgenic rice resulted in a significant increase in total P content in seed, due to increases in InsP6 and inorganic phosphates. On the other hand, overexpression of genes that catalyze the first and last steps of InsP6 biosynthesis could not increase total P levels. From the experiments using developing seeds, it is suggested that the activation of InsP6 biosynthesis in both very early and very late periods of seed development increases the influx of P from vegetative organs into seeds. This is the first report from a study attempting to elevate the P levels of seed through a transgenic approach.

  7. Phytic acid as a potential treatment for alzheimer's pathology: evidence from animal and in vitro models.

    Science.gov (United States)

    Anekonda, Thimmappa S; Wadsworth, Teri L; Sabin, Robert; Frahler, Kate; Harris, Christopher; Petriko, Babett; Ralle, Martina; Woltjer, Randy; Quinn, Joseph F

    2011-01-01

    Alzheimer's disease (AD) causes progressive, age-dependent cortical and hippocampal dysfunction leading to abnormal intellectual capacity and memory. We propose a novel protective treatment for AD pathology with phytic acid (inositol hexakisphosphate), a phytochemical found in food grains and a key signaling molecule in mammalian cells. We evaluated the protective and beneficial effects of phytic acid against amyloid-β (Aβ) pathology in MC65 cells and the Tg2576 mouse model. In MC65 cells, 48-72-hour treatment with phytic acid provided complete protection against amyloid precursor protein-C-terminal fragment-induced cytotoxicity by attenuating levels of increased intracellular calcium, hydrogen peroxide, superoxide, Aβ oligomers, and moderately upregulated the expression of autophagy (beclin-1) protein. In a tolerance paradigm, wild type mice were treated with 2% phytic acid in drinking water for 70 days. Phytic acid was well tolerated. Ceruloplasmin activity, brain copper and iron levels, and brain superoxide dismutase and ATP levels were unaffected by the treatment. There was a significant increase in brain levels of cytochrome oxidase and a decrease in lipid peroxidation with phytic acid administration. In a treatment paradigm, 12-month old Tg2576 and wild type mice were treated with 2% phytic acid or vehicle for 6 months. Brain levels of copper, iron, and zinc were unaffected. The effects of phytic acid were modest on the expression of AβPP trafficking-associated protein AP180, autophagy-associated proteins (beclin-1, LC3B), sirtuin 1, the ratio of phosphorylated AMP-activated protein kinase (PAMPK) to AMPK, soluble Aβ1-40, and insoluble Aβ1-42. These results suggest that phytic acid may provide a viable treatment option for AD.

  8. The reduction process of phytic acid silver ion system: A pulse radiolysis study

    Science.gov (United States)

    Joshi, Ravi; Mukherjee, Tulsi

    2007-05-01

    Reduction of silver ion in a silver-phytic acid (1:1 ratio) system has been studied using pulse radiolysis technique. Time-resolved transformation of the intermediates, Ag +→Ag 0→Ag 2+→Ag 32+, has been clearly observed in the reduction of silver-phytic acid (1:1) system. The effect of phytic acid on the formation and decay of initial silver clusters has been also studied. The surface plasmon absorption band of stable silver nanoparticle (410 nm) and dynamic light scattering technique has been used to characterize the nanoparticles and measure the average size ( Rav=100 nm).

  9. Protection of NdFeB magnets by corrosion resistance phytic acid conversion film

    Science.gov (United States)

    Nan, Haiyang; Zhu, Liqun; Liu, Huicong; Li, Weiping

    2015-11-01

    Phytic acid conversion film was prepared on NdFeB magnets by dipping the NdFeB into phytic acid solution. The morphology, composition, structure and corrosion resistance of the film were systematically investigated. The results showed that the phytic acid film was effective in improving the corrosion resistance of NdFeB magnets. XRD, TEM and FT-IR analyses revealed that the film was amorphous and had a strong peak of phosphate radical (PO43-). The formation mechanism of the film was also explored by XPS and the potential of zero charge (Epzc) measurement at the solution-metal interface.

  10. Phytic acid protects porcine intestinal epithelial cells from deoxynivalenol (DON) cytotoxicity.

    OpenAIRE

    Pacheco, Graziela Drociunas; Silva,Caio Abércio da; Pinton, Philippe; Oswald, Isabelle

    2012-01-01

    The purpose of this study was to evaluate the effects of phytic acid (IP(6)) as a possible inhibitor of cellular damage induced by toxic substances such as mycotoxins on a porcine intestinal epithelial cell line (IPEC-1). We first observed that a dose of 5 mM phytic acid decreases cell viability and transepithelial electrical resistance (TEER) of cell monolayer. We next investigate the effect of non-cytotoxic dose of phytic acid on the deoxinivalenol (DON) induced decreased TEER. We showed th...

  11. Identification and Characterization of Late Pathway Enzymes in Phytic Acid Biosynthesis in Glycine max

    OpenAIRE

    Stiles, Amanda Rose

    2007-01-01

    Phytic acid, also known as myo-inositol hexakisphosphate or Ins(1,2,3,4,5,6)P6, is the major storage form of phosphorus in plant seeds. Phytic acid is poorly digested by non-ruminant animals such as swine and poultry, and it chelates mineral cations including calcium, iron, zinc, and potassium, classifying it as an anti-nutrient. The excretion of unutilized phytic acid in manure translates to an excess amount of phosphorus runoff that can lead to eutrophication of lakes and ponds. Understand...

  12. Phytic acid and myo-inositol support adipocyte differentiation and improve insulin sensitivity in 3T3-L1 cells.

    Science.gov (United States)

    Kim, Jin Nam; Han, Sung Nim; Kim, Hye-Kyeong

    2014-08-01

    Phytic acid, also known as myo-inositol hexaphosphate, has been shown to lower blood glucose levels and to improve insulin sensitivity in rodents. We investigated the effects of phytic acid and myo-inositol on differentiation, insulin-stimulated glucose uptake, and lipolysis of adipocytes to test the hypothesis that the antidiabetic properties of phytic acid and myo-inositol are mediated directly through adipocytes. 3T3-L1 cells were treated with 10, 50, or 200 μmol/L of phytic acid or myo-inositol. Oil Red O staining and an intracellular triacylglycerol assay were used to determine lipid accumulation during adipocyte differentiation. Immunoblotting and real-time polymerase chain reaction (PCR) were performed to evaluate expression of transcription factors, a target protein, and insulin signaling molecules. Phytic acid and myo-inositol exposures increased lipid accumulation in a dose-dependent manner (P acid synthase increased upon treatments with phytic acid and myo-inositol (P phytic acid and myo-inositol treatments (P phytic acid and myo-inositol treatments. In fully differentiated adipocytes, phytic acid and myo-inositol reduced basal lipolysis dose dependently (P phytic acid and myo-inositol increase insulin sensitivity in adipocytes by increasing lipid storage capacity, improving glucose uptake, and inhibiting lipolysis.

  13. Electroless Ni-P plating with a phytic acid pretreatment on AZ91D magnesium alloy

    Energy Technology Data Exchange (ETDEWEB)

    Cui Xiufang, E-mail: cuixf97721@yahoo.com.cn [School of Materials Science and Chemical Engineering, Harbin Engineering University, 145 Nantong St., Harbin 150001 (China); State Key Laboratory for Corrosion and Protection, Institute of Metal Research, Chinese Academy of Sciences, 62 Wencui Road, Shenyang 110016 (China); Jin Guo; Li Qingfen; Yang Yuyun [School of Materials Science and Chemical Engineering, Harbin Engineering University, 145 Nantong St., Harbin 150001 (China); Li Ying [State Key Laboratory for Corrosion and Protection, Institute of Metal Research, Chinese Academy of Sciences, 62 Wencui Road, Shenyang 110016 (China); Wang Fuhui [School of Materials Science and Chemical Engineering, Harbin Engineering University, 145 Nantong St., Harbin 150001 (China); State Key Laboratory for Corrosion and Protection, Institute of Metal Research, Chinese Academy of Sciences, 62 Wencui Road, Shenyang 110016 (China)

    2010-05-15

    A phytic acid conversion film with especial functional groups was proposed as the pretreatment layer between Ni-P coating and AZ91D magnesium alloy substrate, to replace the traditional pretreatment. In the process, the silane coupling agent was adopted as connector between conversion film and palladium ion with catalysis. The microstructure of the phytic acid conversion coatings was observed using scanning electronic microscopy, while the composition and functional groups were analyzed by energy dispersive spectrometer and Fourier transform infrared spectroscopy. The bonding between Si-OH of the silane coupling agent and hydroxyl of phytic acid was validated by X-ray photoelectron spectroscopy, and the existence of palladium ion was also verified. The subsequent Ni-P deposited on the layer was also characterized by its structure, morphology, and corrosion resistance. The results show that the Ni-P coatings with the phytic acid pretreatment on AZ91D magnesium alloy have good corrosion resistance.

  14. Differences in phytase activity and phytic acid content between cultivated and Tibetan annual wild barleys.

    Science.gov (United States)

    Dai, Fei; Qiu, Long; Xu, Yang; Cai, Shengguan; Qiu, Boyin; Zhang, Guoping

    2010-11-24

    The Qinghai-Tibetan Plateau in China is considered to be one of the original centers of cultivated barley. At present, little is known about the phytase activity (Phy) or phytic acid content (PA) in grains of Tibetan annual wild barley. Phy and PA were determined in grains of 135 wild and 72 cultivated barleys. Phy ranged from 171.3 to 1299.2 U kg(-1) and from 219.9 to 998.2 U kg(-1) for wild and cultivated barleys, respectively. PA and protein contents were much higher in wild barley than in cultivated barley. Tibetan annual wild barley showed a larger genetic diversity in phytase activity and phytic acid and protein contents and is of value for barley breeding. There is no significant correlation between phytase activity and phytic acid or protein content in barley grains, indicating that endogenous phytase activity had little effect on the accumulation of phytic acid.

  15. Phytic acid and raffinose series oligosaccharides metabolism in developing chickpea seeds.

    Science.gov (United States)

    Zhawar, Vikramjit Kaur; Kaur, Narinder; Gupta, Anil Kumar

    2011-10-01

    Phytic acid and raffinose series oligosaccharides (RFOs) have anti-nutritional properties where phytic acid chelates minerals and reduces their bioavailability to humans and other animals, and RFOs cause flatulence. Both phytic acid and RFOs cannot be digested by monogastric animals and are released as pollutant-wastes. Efforts are being made to reduce the contents of these factors without affecting the viability of seeds. This will require a thorough understanding of their metabolism in different crops. Biosynthetic pathways of both metabolites though are interlinked but not well described. This study was made on metabolism of these two contents in developing chickpea (Cicer arietinum L cv GL 769) seeds. In this study, deposition of RFOs was found to occur before deposition of phytic acid. A decline in inorganic phosphorus and increase in phospholipid phosphorus and phytic acid was observed in seeds during development. Acid phosphatase was the major phosphatase in seed as well as podwall and its activity was highest at early stage of development, thereafter it decreased. Partitioning of (14) C label from (14) C-glucose and (14) C-sucrose into RFOs and phytic acid was studied in seeds in presence of inositol, galactose and iositol and galactose, which favored the view that galactinol synthase is not the key enzyme in RFOs synthesis.

  16. Phenotypic, genetic and molecular characterization of a maize low phytic acid mutant (lpa241)

    DEFF Research Database (Denmark)

    Pilu, R.; Panzeri, D.; Gavazzi, G.

    2003-01-01

    90% reduction of phytic acid and about a tenfold increase in seed-free phosphate content. Although germination rate was decreased by about 30% compared to wild-type, developement of mutant plants was apparentely unaffected. The results of the genetic, biochemical and molecular characterization...... experiments carried out by SSR mapping, MDD-HPLC and RT-PCR are consistent with a mutation affecting the MIPS1S gene, coding for the first enzyme of the phytic acid biosynthetic pathway....

  17. Fluorimetric determination of phytic acid in urine based on replacement reaction.

    Science.gov (United States)

    Chen, Yingyu; Chen, Jingwen; Ma, Kang; Cao, Shuhong; Chen, Xiaoqing

    2007-12-19

    A sensitive fluorimetric method for determination of phytic acid in human urine samples was described. The method was based on a fluorimetric replacement reaction, in which the added phytic acid replaced the Cu2+ ion from Cu2+-gelatin complex, liberating the fluorescent gelatin molecule. The fluorescence of the solution was accordingly recovered proportionally to the amount of the foreign phytic acid. The excitation wavelength was 273.5 nm and the characteristic emission wavelength was 305.0 nm, respectively. The calibration graph was obtained by plotting the recovered fluorescent intensity at maximum 305.0 nm against the added standard phytic acid, and was divided into two sections. One section was linear over the range of 0.40-2.40 mg L(-1) with a linear regression equation of I(f) = -0.895+15.146c (R2 > 0.9993), and the other over the range of 2.40-9.20 mg L(-1) with a linear regression equation of I(f) = -29.526+26.113c (R2 > 0.9996), respectively. The relative standard deviation (R.S.D.) at 95% confidence degree for a 2.0 mg L(-1) of standard phytic acid within 1 month was less than 1.26% (n = 5), indicating the procedure is reproducible. The detection and the quantification limits of phytic acid were estimated to be 0.23 and 0.40 mg L(-1), respectively. The proposed method was applied to the determination of phytic acid in urine samples and the found concentrations of phytic acid in urine were in the range of 0.49-0.75 mg L(-1) with recoveries of 96.2-108.8%. Comparison of the obtained results with the reported HPLC was performed, indicating the proposed method was reliable.

  18. Phenotypic, genetic and molecular characterization of a maize low phytic acid mutant (lpa241).

    Science.gov (United States)

    Pilu, R; Panzeri, D; Gavazzi, G; Rasmussen, S K; Consonni, G; Nielsen, E

    2003-10-01

    Phytic acid, myo-inositol 1,2,3,4,5,6-hexakisphosphate, is the major storage compound of phosphorous (P) in plants, predominantly accumulating in seeds (up to 4-5% of dry weight) and pollen. In cereals, phytic acid is deposited in embryo and aleurone grain tissues as a mixed "phytate" salt of potassium and magnesium, although phytates contain other mineral cations such as iron and zinc. During germination, phytates are broken down by the action of phytases, releasing their P, minerals and myo-inositol which become available to the growing seedling. Phytic acid represents an anti-nutritional factor for animals, and isolation of maize low phytic acid ( lpa) mutants provides a novel approach to study its biochemical pathway and to tackle the nutritional problems associated with it. Following chemical mutagenesis of pollen, we have isolated a viable recessive mutant named lpa 241 showing about 90% reduction of phytic acid and about a tenfold increase in seed-free phosphate content. Although germination rate was decreased by about 30% compared to wild-type, developement of mutant plants was apparentely unaffected. The results of the genetic, biochemical and molecular characterization experiments carried out by SSR mapping, MDD-HPLC and RT-PCR are consistent with a mutation affecting the MIPS1S gene, coding for the first enzyme of the phytic acid biosynthetic pathway.

  19. Embryo-specific silencing of a transporter reduces phytic acid content of maize and soybean seeds.

    Science.gov (United States)

    Shi, Jinrui; Wang, Hongyu; Schellin, Kathleen; Li, Bailin; Faller, Marianna; Stoop, Johan M; Meeley, Robert B; Ertl, David S; Ranch, Jerry P; Glassman, Kimberly

    2007-08-01

    Phytic acid in cereal grains and oilseeds is poorly digested by monogastric animals and negatively affects animal nutrition and the environment. However, breeding programs involving mutants with less phytic acid and more inorganic phosphate (P(i)) have been frustrated by undesirable agronomic characteristics associated with the phytic acid-reducing mutations. We show that maize lpa1 mutants are defective in a multidrug resistance-associated protein (MRP) ATP-binding cassette (ABC) transporter that is expressed most highly in embryos, but also in immature endosperm, germinating seed and vegetative tissues. Silencing expression of this transporter in an embryo-specific manner produced low-phytic-acid, high-Pi transgenic maize seeds that germinate normally and do not show any significant reduction in seed dry weight. This dominant transgenic approach obviates the need for incorporating recessive lpa1 mutations to create maize hybrids with reduced phytic acid. Suppressing the homologous soybean MRP gene also generated low-phytic-acid seed, suggesting that the strategy might be feasible for many crops.

  20. Protection of copper surface with phytic acid against corrosion in chloride solution.

    Science.gov (United States)

    Peca, Dunja; Pihlar, Boris; Ingrid, Milošev

    2014-01-01

    Phytic acid (inositol hexaphosphate) was tested as a corrosion inhibitor for copper in 3% sodium chloride. Phytic acid is a natural compound derived from plants, it is not toxic and can be considered as a green inhibitor. Electrochemical methods of linear polarization and potentiodynamic polarization were used to study the electrochemical behaviour and evaluate the inhibition effectiveness. To obtain the optimal corrosion protection the following experimental conditions were investigated: effect of surface pre-treatment (abrasion and three procedures of surface roughening), pre-formation of the layer of phytic acid, time of immersion and concentration of phytic acid. To evaluate the surface pre-treatment procedures the surface roughness and contact angle were measured. Optimal conditions for formation of phytic layer were selected resulting in the inhibition effectiveness of nearly 80%. Morphology and composition of the layer were further studied by scanning electron microscopy, energy dispersive X-ray spectroscopy and X-ray photoelectron spectroscopy. The layer of phytic acid with thickness in the nanometer range homogeneously covers the copper surface. The obtained results show that this natural compound can be used as a mildly effective corrosion inhibitor for copper in chloride solution.

  1. Low phytic acid 1 mutation in maize modifies density, starch properties, cations, and fiber contents in the seed.

    Science.gov (United States)

    Landoni, Michela; Cerino Badone, Francesco; Haman, Nabil; Schiraldi, Alberto; Fessas, Dimitrios; Cesari, Valentina; Toschi, Ivan; Cremona, Roberta; Delogu, Chiara; Villa, Daniela; Cassani, Elena; Pilu, Roberto

    2013-05-15

    Monogastric animals are unable to digest phytic acid, so it represents an antinutritional factor and also an environmental problem. One strategy to solve this problem is the utilization of low phytic acid (lpa) mutants that accumulate low levels of phytic P and high levels of free phosphate in the seeds; among the lpa maize mutants lpa1 exhibited the highest reduction of phytic acid in the seed. This study indicated that the low phytic acid mutations exerted pleiotropic effects not directly connected to the phytic acid pathway, such as on seed density, content of ions, and the antioxidant compounds present in the kernels. Furthermore some nutritional properties of the flour were altered by the lpa1 mutations, in particular lignin and protein content, while the starch does not seem to be modified as to the total amount and in the amylose/amylopectin ratio, but alterations were noticed in the structure and size of granules.

  2. A Novel and Rapid Colorimetric Method for Measuring Total Phosphorus and Phytic Acid in Foods and Animal Feeds.

    Science.gov (United States)

    2016-04-08

    Phytic acid, or myo-inositol hexakisphosphate, is the primary source of inositol and storage phosphorus in plant seeds and has considerable nutritional importance. In this form, phosphorus is unavailable for absorption by monogastric animals, and the strong chelating characteristic of phytic acid reduces the bioavailability of multivalent minerals such as iron, zinc, and calcium. Currently, there is no simple quantitative method for phytic acid; existing methods are complex, and the most commonly accepted method, AOAC Official Method (SM) 986.11, has limitations. The aim of this work was to develop and validate a simple, high-throughput method for the measurement of total phosphorus and phytic acid in foods and animal feeds. The method described here involves acid extraction of phytic acid, followed by dephosphorylation with phytase and alkaline phosphatase. The phosphate released from phytic acid is measured using a modified colorimetric molybdenum blue assay and calculated as total phosphorus or phytic acid content of the original sample. The method was validated to a maximum linearity of 3.0 g phytic acid/100 g sample. Accuracy ranged from 98 to 105% using pure phytic acid and from 97 to 115% for spiked samples. Repeatability ranged from 0.81 to 2.32%, and intermediate precision was 2.27%.

  3. Evaluation of alternatives for human lysozyme purification from transgenic rice: impact of phytic acid and buffer.

    Science.gov (United States)

    Wilken, Lisa R; Nikolov, Zivko L

    2010-01-01

    Producing economically competitive recombinant human lysozyme from transgenic rice demands an inexpensive purification process for nonpharmaceutical applications. Human lysozyme is a basic protein, and thus, cation exchange chromatography was the selected method for lysozyme purification. Similar to other protein production systems, the identification of critical impurities in the rice extract was important for the development of an efficient purification process. Previous adsorption data indicated that phytic acid was probably responsible for an unacceptably low cation exchange adsorption capacity. In this study, we confirm that reducing phytic acid concentration improves lysozyme binding capacity and investigate alternative process conditions that reduce phytic acid interference. Compared with the previous best process, the adsorption capacity of human lysozyme was increased from 8.6 to 19.7 mg/mL when rice extract was treated with phytase to degrade phytic acid. Using tris buffer to adjust pH 4.5 extract to pH 6 before adsorption reduced phytic acid interference by minimizing phytic acid-lysozyme interactions, eliminated the need for phytase treatment, and increased the binding capacity to 25 mg/mL. Another method of reducing phytic acid concentration was to extract human lysozyme from rice flour at pH 10 with 50 mM NaCl in 50 mM sodium carbonate buffer. A similar binding capacity (25.5 mg/mL) was achieved from pH 10 extract that was clarified by acidic precipitation and adjusted to pH 6 for adsorption. Lysozyme purities ranged from 95 to 98% for all three processing methods. The tris-mediated purification was the most efficient of the alternatives considered.

  4. Probing Phosphorus Efficient Low Phytic Acid Content Soybean Genotypes with Phosphorus Starvation in Hydroponics Growth System.

    Science.gov (United States)

    Kumar, Varun; Singh, Tiratha Raj; Hada, Alkesh; Jolly, Monica; Ganapathi, Andy; Sachdev, Archana

    2015-10-01

    Phosphorus is an essential nutrient required for soybean growth but is bound in phytic acid which causes negative effects on both the environment as well as the animal nutrition. Lowering of phytic acid levels is associated with reduced agronomic characteristics, and relatively little information is available on the response of soybean plants to phosphorus (P) starvation. In this study, we evaluated the effects of different P starvation concentrations on the phytic acid content, growth, and yield of seven mutant genotypes along with the unirradiated control, JS-335, in a hydroponics growth system. The low phytic acid containing mutant genotypes, IR-JS-101, IR-DS-118, and IR-V-101, showed a relatively high growth rate in low P concentration containing nutrient solution (2 μM), whereas the high P concentration (50 μM) favored the growth of IR-DS-111 and IR-DS-115 mutant genotypes containing moderate phytate levels. The mutant genotypes with high phytic acid content, IR-DS-122, IR-DS-114, and JS-335, responded well under P starvation and did not have any significant effect on the growth and yield of plants. Moreover, the reduction of P concentration in nutrient solution from 50 to 2 μM also reduced the phytic acid content in the seeds of all the soybean genotypes under study. The desirable agronomic performance of low phytic acid containing mutant genotype IR-DS-118 reported in this study suggested it to be a P-efficient genotype which could be considered for agricultural practices under P limiting soils.

  5. Impact of phytic acid on nutrient bioaccessibility and antioxidant properties of dehusked rice.

    Science.gov (United States)

    Lee, H H; Loh, S P; Bong, C F J; Sarbini, S R; Yiu, P H

    2015-12-01

    Whole grains consumption promotes health benefits, but demonstrates controversial impacts from phytic acid in meeting requirements of good health. Therefore, this study was aimed to determine the nutrient bioaccessibility and antioxidant properties of rice cultivars named "Adan" or "Bario" and deduce the nutritional impact of phytic acid. Majority of the dehusked rice in the collection showed an acceptable level of in-vitro starch digestibility and in-vitro protein digestibility, but were poor in antioxidant properties and bioaccessibility of minerals (Ca, Fe and Zn). The drawbacks identified in the rice cultivars were due to relatively high phytic acid content (2420.6 ± 94.6 mg/100 g) and low phenolic content (152.39 ± 18.84 μg GAE/g). The relationship between phytic acid content and mineral bioaccessibility was strongest in calcium (r = 0.60), followed by iron (r = 0.40) and zinc (r = 0.27). Phytic acid content did not significantly correlate with in-vitro starch digestibility and in-vitro protein digestibility but showed a weak relationship with antioxidant properties. These suggest that phytic acid could significantly impair the mineral bioaccessibility of dehusked rice, and also act as an important antioxidant in non-pigmented rice. Bario rice cultivars offered dehusked rice with wide range of in-vitro digestibility of starch and protein, and also pigmented rice as a good source of antioxidants. However, there is a need to reduce phytic acid content in dehusked rice for improved mineral bioaccessibility among Bario rice cultivars.

  6. Phytic acid: an alternative root canal chelating agent.

    Science.gov (United States)

    Nassar, Mohannad; Hiraishi, Noriko; Tamura, Yukihiko; Otsuki, Masayuki; Aoki, Kazuhiro; Tagami, Junji

    2015-02-01

    The objectives of this study were to investigate the effect of phytic acid, inositol hexakisphosphate (IP6), as a final rinse on the surface of instrumented root canals and smear-layered flat dentin surfaces treated with sodium hypochlorite (NaOCl) and to evaluate its effect on the viability and alkaline phosphatase activity of osteoblast-like cells (MC3T3-E1). The universally accepted chelating agent EDTA was used as the control in all conducted experiments. Root canals of human canines were instrumented with rotary files and irrigated with 5% NaOCl, followed by a final rinse of 17% EDTA (1 minute), 1% IP6 (1 minute or 30 seconds), or distilled water. NaOCl-treated flat coronal dentin surfaces were also treated with 17% EDTA (1 minute), 1% IP6 (1 minute or 30 seconds), or distilled water. The presence or absence of smear layer was evaluated with scanning electron microscopy. Cell viability and alkaline phosphatase assays were performed to evaluate the effect of IP6 and EDTA on cultured MC3T3-E1 cells. The results demonstrated the ability of IP6 to remove the smear layer from instrumented root canals and flat coronal dentin surfaces. When compared with EDTA, IP6 was less cytotoxic and did not affect the differentiation of MC3T3-E1 cells. IP6 shows the potential to be an effective and biocompatible chelating agent. Copyright © 2015 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  7. Effect of dietary phytic acid on performance and nutrient uptake in the small intestine of piglets.

    Science.gov (United States)

    Woyengo, T A; Weihrauch, D; Nyachoti, C M

    2012-02-01

    An experiment was conducted with piglets to determine the effect of dietary phytic acid supplementation on performance, electrophysiological properties of jejunum mounted in Ussing chambers, sodium-dependent glucose transporter 1 (SGLT1) protein expression in jejunum, and plasma glucose and Na concentrations. Sixteen piglets with an average initial BW of 7.40 ± 0.36 kg were randomly assigned to 2 experimental diets with 8 piglets per diet. The diets were casein-cornstarch-based and were either unsupplemented or supplemented with 2% phytic acid (as Na phytate). The basal diet was formulated to meet the recommendation of NRC (1998) for energy, AA, minerals, and vitamins for piglets. The experiment lasted for 21 d, and at the end, BW gain and feed consumption were determined, and blood samples were collected for determination of plasma glucose and Na concentrations. The piglets were then euthanized to determine jejunal electrophysiological properties (transmural potential difference and short-circuit current) and SGLT1 protein expression. Phytic acid supplementation reduced ADG (P = 0.002), ADFI (P = 0.017), and G:F (P = 0.001) from 316.1 to 198.2 g, 437.4 to 360.3 g, and 0.721 to 0.539 g/g, respectively. Phytic acid supplementation also tended to reduce (P = 0.088) potential difference (-3.80 vs. -2.23 mV) and reduced (P = 0.023) short-circuit current from 8.07 to 0.1 μA/cm(2). However, phytic acid supplementation did not affect SGLT1 protein, and blood plasma glucose and Na concentrations. In conclusion, dietary phytic acid reduced growth performance and transmural short-circuit current in the jejunum of piglets. The reduced transmural short-circuit current in the jejunum by phytic acid implies reduced active Na transport in the jejunum by the phytic acid. Therefore, it seems that dietary phytic acid reduces growth performance of pigs partly through reduced capacity of the small intestine to absorb Na.

  8. Phytic acid (IP6), novel broad spectrum anti-neoplastic agent: a systematic review.

    Science.gov (United States)

    Fox, C H; Eberl, M

    2002-12-01

    Phytic acid or IP6 has been extensively studied in animals and is being promoted as an anti-cancer agent in health food stores. It is naturally found in legumes, wheat bran, and soy foods. It is believed to be the active ingredient that gives these substances their cancer fighting abilities. Proposed mechanisms of action include gene alteration, enhanced immunity, and anti-oxidant properties. A Medline search from 1966 to May 2002 using the keywords phytic acid and cancer, and limiting the search to the subheadings of therapeutic uses, prevention, and adverse effects revealed 28 studies. These studies were included in the review. A great majority of the studies were done in animals and showed that phytic acid had anti-neoplastic properties in breast, colon, liver, leukemia, prostate, sarcomas, and skin cancer. There were no human studies. Side effects included chelation of multivalent cations, and an increase in bladder and renal papillomas. This increase in papilloma formation only occurred with the sodium salt of phytic acid. It did not occur with either the potassium or magnesium salts. There is a large body of animal evidence to show that phytic acid may have a role in both the prevention and treatment of many forms of cancer. There is clearly enough evidence to justify the initiation of Phase I and Phase II clinical trials in humans.

  9. Potentiometric Determination of Phytic Acid and Investigations of Phytate Interactions with Some Metal Ions.

    Science.gov (United States)

    Marolt, Gregor; Pihlar, Boris

    2015-01-01

    Determination of correct amount (concentration) of phytic acid is of vital importance when dealing with protonation and/or metal complexation equilibria. A novel approach for precise and reliable assay of phytic acid, based on the difference between end points by potentiometric titration, has been presented. Twelve phytic acid protons are classified into three groups of acidity, which enables detection of 2 to 3 distinct equivalent points (EPs) depending on experimental conditions, e.g. counter-ion concentration. Using the differences between individual EPs enables correct phytate determination as well as identification of potential contamination and/or determination of initial protonation degree. Impact of uncertainty of phytate amount on the calculation of protonation constants has been evaluated using computer simulation program (Hyperquad2013). With the analysis of titration curves different binding sites on phytate ligand have been proposed for complexation of Ca2+ and Fe3+ ions.

  10. Induction of phytic acid synthesis by abscisic acid in suspension-cultured cells of rice.

    Science.gov (United States)

    Matsuno, Koya; Fujimura, Tatsuhito

    2014-03-01

    A pathway of phytic acid (PA) synthesis in plants has been revealed via investigations of low phytic acid mutants. However, the regulation of this pathway is not well understood because it is difficult to control the environments of cells in the seeds, where PA is mainly synthesized. We modified a rice suspension culture system in order to study the regulation of PA synthesis. Rice cells cultured with abscisic acid (ABA) accumulate PA at higher levels than cells cultured without ABA, and PA accumulation levels increase with ABA concentration. On the other hand, higher concentrations of sucrose or inorganic phosphorus do not affect PA accumulation. Mutations in the genes RINO1, OsMIK, OsIPK1 and OsLPA1 have each been reported to confer low phytic acid phenotypes in seeds. Each of these genes is upregulated in cells cultured with ABA. OsITPK4 and OsITPK6 are upregulated in cells cultured with ABA and in developing seeds. These results suggest that the regulation of PA synthesis is similar between developing seeds and cells in this suspension culture system. This system will be a powerful tool for elucidating the regulation of PA synthesis.

  11. Isolation and characterization of a low phytic acid rice mutant reveals a mutation in the rice orthologue of maize mik.

    Science.gov (United States)

    Using a forward genetics approach, we isolated two independent low phytic acid (lpa) rice mutants, N15-186 and N15-375. Both mutants are caused by single gene, recessive non-lethal mutations which result in approximately 75% (N15-186) and 43% (N15-375) reductions in seed phytic acid (inositol hexaki...

  12. Identification of the Molecular Basis of the Seed Low Phytic Acid Phenotype in Soybean Line CX1834

    Science.gov (United States)

    Plant seeds accumulate phosphorus in the form of myo-inositol-1,2,3,4,5,6 hexakisphosphate, commonly referred to as phytic acid. Phytic acid is complexed with cationic mineral species in the form of phytate, which is not well digested or absorbed by monogastric species such as humans, poultry and sw...

  13. Reduction of polyphenol and phytic acid content of pearl millet grains by malting and blanching.

    Science.gov (United States)

    Archana; Sehgal, S; Kawatra, A

    1999-01-01

    This work was undertaken to evaluate the changes in polyphenol and phytic acid content in malted and blanched pearl millet grains. For malting, grains were steeped for 16 hours, germinated for 48 or 72 hours and then kilned at 50 degrees C for 24 hours. Blanching was done for 30 seconds in boiling water at 98 degrees C. Results indicated that blanching resulted in significant reduction in polyphenol (28%) and phytic acids (38%). Destruction of polyphenols (38 to 48%) and phytic acid (46 to 50%) was significantly higher in grains subjected to malting than blanching: The overall results suggested that malting with 72 hours of germination was most effective in reducing the antinutrient levels of pearl millet grains.

  14. The reduction process of phytic acid-silver ion system: A pulse radiolysis study

    Energy Technology Data Exchange (ETDEWEB)

    Joshi, Ravi [Radiation and Photochemistry Division, Chemistry Group, Bhabha Atomic Research Center, Mumbai 400 085 (India)]. E-mail: rjudrin@yahoo.com; Mukherjee, Tulsi [Radiation and Photochemistry Division, Chemistry Group, Bhabha Atomic Research Center, Mumbai 400 085 (India)

    2007-05-15

    Reduction of silver ion in a silver-phytic acid (1:1 ratio) system has been studied using pulse radiolysis technique. Time-resolved transformation of the intermediates, Ag{sup +{yields}}Ag{sup 0{yields}}Ag{sub 2} {sup +{yields}}Ag{sub 3} {sup 2+}, has been clearly observed in the reduction of silver-phytic acid (1:1) system. The effect of phytic acid on the formation and decay of initial silver clusters has been also studied. The surface plasmon absorption band of stable silver nanoparticle (410 nm) and dynamic light scattering technique has been used to characterize the nanoparticles and measure the average size (R {sub av}=100 nm)

  15. [Effect of phytic acid and its derivatives on blood lipid peroxidation state in vitro].

    Science.gov (United States)

    Martusevich, A K; Sidorova, M V; Mel'nikova, N B; Solov'eva, A G; Peretiagin, S P

    2014-01-01

    We have studied specific features of lipid peroxidation in whole human blood under the action of aqueous solutions of xymedone (19.6 microM), phytic acid (117.9 microM) and its complex (237.6 microM) synthesized in distilled water and isotonic (0.9%) solution of sodium chloride. The estimated parameters included lipid peroxidation (LPO) rate, total antioxidant potential, superoxide dismutase (SOD) level, and malonic dialdehyde (MDA) level in blood plasma and erythrocytes. It was established that the effect of phytic acid on blood samples includes moderate stimulation of total antioxidant activity and SOD activity with predominant prooxidant effect. The phytic acid--xymedone complex synthesized in distilled water exhibits an antioxidant action, while its synthesis in saline solution yields a prooxidant.

  16. Influence of phytic acid and its metal complexes on the activity of pectin degrading polygalacturonase.

    Science.gov (United States)

    Asghar, Uzma; Rehman, Haneef Ur; Qader, Shah Ali Ul; Maqsood, Zahida Tasneem

    2013-06-05

    Polygalacturonase is one of the important requirements of different microorganism to cause pathogenicity and spoilage of fruits and vegetables that involved in degradation of pectin during plant tissue infections. In current study, 20 mM phytic acid inhibited 70% activity of polygalacturonase. The effect of different concentration of metal ions such as Cu(+2), Al(+3) and V(+4) were studied separately and it was found that the 20 mM of these metal ions inhibited 37.2%, 79%, and 53% activity of polygalacturonase, respectively. Finally, the complexes of phytic acid and these metals ions were prepared and 1:1 ratio of phytic acid and metal ions complexes showed maximum inhibitory activity of enzyme as compared to complexes having 1:2 and 1:3 ratio except phytate-copper complexes which showed no inhibitory effect on the activity of polygalacturonase.

  17. Reduction of phytic acid and enhancement of bioavailable micronutrients in food grains.

    Science.gov (United States)

    Gupta, Raj Kishor; Gangoliya, Shivraj Singh; Singh, Nand Kumar

    2015-02-01

    More than half of the world populations are affected by micronutrient malnutrition and one third of world's population suffers from anemia and zinc deficiency, particularly in developing countries. Iron and zinc deficiencies are the major health problems worldwide. Phytic acid is the major storage form of phosphorous in cereals, legumes, oil seeds and nuts. Phytic acid is known as a food inhibitor which chelates micronutrient and prevents it to be bioavailabe for monogastric animals, including humans, because they lack enzyme phytase in their digestive tract. Several methods have been developed to reduce the phytic acid content in food and improve the nutritional value of cereal which becomes poor due to such antinutrient. These include genetic improvement as well as several pre-treatment methods such as fermentation, soaking, germination and enzymatic treatment of grains with phytase enzyme. Biofortification of staple crops using modern biotechnological techniques can potentially help in alleviating malnutrition in developing countries.

  18. Effect of Post Heat Treatment on Corrosion Resistance of Phytic Acid Conversion Coated Magnesium

    Institute of Scientific and Technical Information of China (English)

    R.K. Gupta; K. Mensah-Darkwa; D. Kumar

    2013-01-01

    An environment friendly chemical conversion coating for magnesium was obtained by using a phytic acid solution.The effect of post-coating 1heat treatment on the microstructures and corrosion properties of phytic acid conversion coated magnesium was investigated.It was observed that the microstructure and corrosion resistive properties were improved for the heat treated samples.The corrosion current density for bare magnesium,phytic acid conversion coated magnesium,and post-coating heat treated magnesium was calculated to be 2.48 × 10-5,1.18 × 10-6,and 9.27 × 10-7 A/cm2,respectively.The lowest corrosion current density for the heat treated sample indicated its highest corrosion resistive effect for the magnesium.The maximum corrosion protective nature of the heat treated sample was further confirmed by the largest value of impedance in electrochemical impedance spectroscopy studies.

  19. Comparison of phosphate estimating methods in the presence of phytic acid for the determination of phytase activity.

    Science.gov (United States)

    Sanikommu, Suma; Pasupuleti, Mukesh; Vadalkonda, Lakshmipathi

    2014-01-01

    Phosphate released from phytic acid can be used as a measure of phytase activity. However, most of the phosphate estimation methods have not examined the interference or interaction of phytic acid in the assay. In this article, we report the kinetics and influence of unreduced phytic acid on phosphate estimation by three of the often-used methods for phytase estimation, the AOAC, Cooper-Gowing, and Fiske-Subbarow methods. Our results show that the AOAC method is most suitable to estimate the phytase activity in the presence of phytate in the medium. In the Fiske and Subbarow method, we noticed that the time factor plays a role in the interference of the phytic acid; especially the readings taken during the second hour of incubation are influenced by the presence of phytic acid. The method of Cooper and Gowing is labor-intensive and is prone to give error values at higher concentrations.

  20. Preparation of Phytic Acid/Silane Hybrid Coating on Magnesium Alloy and Its Corrosion Resistance in Simulated Body Fluid

    Science.gov (United States)

    Wang, Fengwu; Cai, Shu; Shen, Sibo; Yu, Nian; Zhang, Feiyang; Ling, Rui; Li, Yue; Xu, Guohua

    2017-09-01

    In order to decrease the corrosion rate and improve the bioactivity of magnesium alloy, phytic acid/saline hybrid coatings were synthesized on AZ31 magnesium alloys by sol-gel dip-coating method. It was found that the mole ratio of phytic acid to γ-APS had a great influence on coating morphology and the corresponding corrosion resistance of the coated magnesium alloys. When the mole ratio of phytic acid to γ-APS was 1:1, the obtained hybrid coating was integral and without cracks, which was ascribed to the strong chelate capability of phytic acid and Si-O-Si network derived from silane. Electrochemical test result indicated that the corrosion resistance of the coated magnesium alloy was about 27 times larger than that of the naked counterpart. In parallel, immersion test showed that the phytic acid/silane hybrid coating could induce CaP-mineralized product deposition, which offered another protection for magnesium alloy.

  1. Influence of dietary phytic acid and source of microbial phytase on ileal endogenous amino acid flows in broiler chickens.

    Science.gov (United States)

    Cowieson, A J; Ravindran, V; Selle, P H

    2008-11-01

    The effects of phytic acid and 2 sources of exogenous phytase (bacterial vs. fungal) on the flow of endogenous amino acids at the terminal ileum of broilers were assessed using the enzyme-hydrolyzed casein method. Phytic acid (as the sodium salt) was included in a purified diet at 8.5 and 14.5 g/kg, and each diet was fed without or with a fungal (Aspergillus niger-derived) or a bacterial (Escherichia coli-derived) microbial phytase at 500 phytase units/kg of diet. Increasing the concentration of phytic acid in the diet from 8.5 to 14.5 g/kg increased (P acids by an average of 68%, with a range from 17% for proline to 145% for phenylalanine. The flow of endogenous aspartic acid, serine, glutamic acid, glycine, leucine, tyrosine, phenylalanine, and histidine were increased by more than the mean, indicating changes in the composition of endogenous protein in response to the presence of higher concentrations of phytic acid. Supplementation of both phytases reduced (P acids, but the reduction (P = 0.06) was greater for the bacterial phytase compared with the fungal phytase. These data suggest that a substantial part of the amino acid and energy responses observed following phytase supplementation in broiler chickens stems from reduced endogenous amino acid flows and that the capacity of different phytases to counteract the antinutritive properties of phytic acid vary.

  2. Compositional changes in trypsin inhibitors, phytic acid, saponins and isoflavones related to soybean processing.

    Science.gov (United States)

    Anderson, R L; Wolf, W J

    1995-03-01

    Soybeans are high in protein but also contain a number of minor constituents traditionally considered to be antinutritional factors. These include trypsin inhibitors, phytic acid, saponins and isoflavones. These compounds are now thought to have beneficial biological effects in the diet, such as lowering blood cholesterol or preventing cancer. Soybean processing changes the content of these minor constituents in various ways. This review discusses the changes in content of trypsin inhibitors, phytic acid, saponins and isoflavones as soybeans are processed into the conventional protein ingredients, flours, concentrates and isolates, as well as some of the traditional Oriental soybean foods.

  3. Evaluation of simple and inexpensive high-throughput methods for phytic acid determination

    Science.gov (United States)

    High-throughput/low-cost/low-tech methods for phytic acid determination that are sufficiently accurate and reproducible would be of value in plant genetics, crop breeding and in the food and feed industries. Variants of two candidate methods, those described by Vaintraub and Lapteva (Anal. Biochem. ...

  4. Evaluation of Simple and Inexpensive High-Throughput Methods for Phytic Acid Determination

    DEFF Research Database (Denmark)

    Raboy, Victor; Johnson, Amy; Bilyeu, Kristin

    2017-01-01

    consistently acceptable (˂2.0”) “Horwitz ratios”, a measure of reproducibility, although some treatments approached that. For example, one variant of the VL method when used to assay a soybean flour with a “standard” level of phytic acid had a Horwitz ratio of 2.15. Some variants of the VL method were adequate...

  5. In situ ruminal degradation of phytic acid in formaldehyde treated rice bran

    NARCIS (Netherlands)

    Martin-Tereso, J.; Gonzalez, A.; Laar, van H.; Burbara, C.; Pedrosa, M.; Mulder, K.; Hartog, den L.A.; Verstegen, M.W.A.

    2009-01-01

    Rice bran has a very high content of phytic acid (IP6), which is a nutritional antagonist of Ca. Microbial phytase degrades IP6, but ruminal degradation of nutrients can be reduced by formaldehyde treatment. Milk fever in dairy cows can be prevented by reducing available dietary Ca to stimulate Ca

  6. Mechanism of Calcium Lactate Facilitating Phytic Acid Degradation in Soybean during Germination.

    Science.gov (United States)

    Hui, Qianru; Yang, Runqiang; Shen, Chang; Zhou, Yulin; Gu, Zhenxin

    2016-07-13

    Calcium lactate facilitates the growth and phytic acid degradation of soybean sprouts, but the mechanism is unclear. In this study, calcium lactate (Ca) and calcium lactate with lanthanum chloride (Ca+La) were used to treat soybean sprouts to reveal the relevant mechanism. Results showed that the phytic acid content decreased and the availability of phosphorus increased under Ca treatment. This must be due to the enhancement of enzyme activity related to phytic acid degradation. In addition, the energy metabolism was accelerated by Ca treatment. The energy status and energy metabolism-associated enzyme activity also increased. However, the transmembrane transport of calcium was inhibited by La(3+) and concentrated in intercellular space or between the cell wall and cell membrane; thus, Ca+La treatment showed reverse results compared with those of Ca treatment. Interestingly, gene expression did not vary in accordance with their enzyme activity. These results demonstrated that calcium lactate increased the rate of phytic acid degradation by enhancing growth, phosphorus metabolism, and energy metabolism.

  7. Phytic acid and inorganic phosphate composition in soybean lines with independent IPK1 mutations

    Science.gov (United States)

    Soybean [Glycine max (L.) Merr] seeds contain a large amount of phosphorus (P), which is stored as phytic acid (PA). PA is indigestible by nonruminent livestock and considered an anti-nutritional factor because PA chelates divalent cations and prevents the uptake of essential nutrients. Interest in...

  8. Electrochemical and Photoelectrochemical Study of Self-assembled Monolayer of Phytic Acid on Brass

    Institute of Scientific and Technical Information of China (English)

    XU Qun-Jie; WAN Zong-Yue; ZHOU Guo-Ding; YIN Ren-He; CAO Wei-Min; LIN Chang-Jian

    2008-01-01

    Phytic acid is an environment-friendly reagent for processing metals.The anticorrosion and inhibiting mechanism for phytic acid monolayers self-assembled on a brass (HSn70-1) electrode has been investigated by using electrochemical and photocurrent response methods.The electrochemical measurements indicate that phytic acid is liable to form surface complexes on the brass electrode,and the self-assembled monolayers (SAM) change the structure of the electric double-layer and shift the potential of zero charge positively.The photochemical measurement indicates that the brass electrode shows a p-type photoresponse owing to the formation of a Cu2O layer on its surface,and the presence of SAM weakens significantly the photoresponse,suggesting an excellent effect on anticorrosion,which is consistent with the EIS and polarization curve measurements.Adsorption of phytic acid was found to be typical of chemisorption,which can be reasonably described on the basis of the Langmuir isotherm.

  9. Diurnal variation in degradation of phytic acid by plant phytase in the pig stomach

    NARCIS (Netherlands)

    Kemme, P.A.; Jongbloed, A.W.; Mroz, Z.; Beynen, A.C.

    1998-01-01

    The effects of plant phytase on the gastric degradation of phytic acid and digestibilities of DM and P, and their diurnal variation were evaluated in pigs from 90 to 115 kg BW fitted with simple duodenal T-cannulas. Three diets were fed to three pigs in four collection periods according to a cross-o

  10. Effect of soaking and phytase treatment on phytic acid, calcium, iron and zinc in rice fractions

    NARCIS (Netherlands)

    Liang, J.; Han, B.Z.; Nout, M.J.R.; Hamer, R.J.

    2009-01-01

    With the aim to maximise phytic acid removal and minimise losses of dry matter and minerals (Ca, Fe, Zn) in rice, three products (whole kernels and flour milled from white and brown rice; and bran, all from the same batch of variety Kenjian 90-31) were soaked in demineralized water at 10 °C (SDW), N

  11. In situ ruminal degradation of phytic acid in formaldehyde treated rice bran

    NARCIS (Netherlands)

    Martin-Tereso, J.; Gonzalez, A.; Laar, van H.; Burbara, C.; Pedrosa, M.; Mulder, K.; Hartog, den L.A.; Verstegen, M.W.A.

    2009-01-01

    Rice bran has a very high content of phytic acid (IP6), which is a nutritional antagonist of Ca. Microbial phytase degrades IP6, but ruminal degradation of nutrients can be reduced by formaldehyde treatment. Milk fever in dairy cows can be prevented by reducing available dietary Ca to stimulate Ca h

  12. Effect of calcium, tannic acid, phytic acid and pectin over iron uptake in an in vitro Caco-2 cell model.

    Science.gov (United States)

    Andrews, M; Briones, L; Jaramillo, A; Pizarro, F; Arredondo, M

    2014-04-01

    Calcium, phytic acid, polyphenols and fiber are major inhibitors of iron absorption and they could be found in excess in some diets, thereby altering or modifying the iron nutrition status. The purpose of this study is to evaluate the effect of calcium, tannic acid, phytic acid, and pectin over iron uptake, using an in vitro model of epithelial cells (Caco-2 cell line). Caco-2 cells were incubated with iron (10-30 μM) with or without CaCl2 (500 and 1,000 μM) for 24 h. Then, cells were challenged with phytic acid (50-150 μM); pectin (50-150 nM) or tannic acid (100-500 μM) for another 24 h. Finally, (55)Fe (10 μM) uptake was determined. Iron dialyzability was studied using an in vitro digestion method. Iron uptake in cells pre-incubated with 20 and 30 μM Fe was inhibited by CaCl2 (500 μM). Iron uptake decreased in cells cultured with tannic acid (300 μM) and CaCl2 (500-1,000 μM) (two-way ANOVA, p = 0.002). Phytic acid also decreased iron uptake mainly when cells were treated with CaCl2 (1,000 μM) (two-way ANOVA; p phytic or tannic acid (T test p Phytic acid combined with calcium is a strong iron uptake inhibitor. Pectin slightly decreased iron uptake with or without calcium. Tannic acid showed an unexpected behavior, inducing an increase on iron uptake, despite its low Fe dialyzability.

  13. Effect of phytic acid and microbial phytase on the flow and amino acid composition of endogenous protein at the terminal ileum of growing broiler chickens.

    Science.gov (United States)

    Cowieson, A J; Ravindran, V

    2007-10-01

    The effects of phytic acid and microbial phytase on the flow and composition of endogenous protein at the terminal ileum of broiler chickens were investigated using the peptide alimentation method. Phytic acid (fed as the sodium salt) was included in a synthetic diet at 8.5, 11.5 and 14.5 g/kg (or 2.4, 3.2 and 4.0 g/kg phytate-phosphorus) and each diet was fed without or with an Escherichia coli-derived microbial phytase at 500 phytase units/kg diet. A control containing no phytate was fed as a comparison to estimate basal endogenous flows. Ingestion of phytic acid increased (P < 0.05) the flow of endogenous amino acids and N by an average of 47 % at the lowest phytic acid concentration and 87 % at the highest. The addition of microbial phytase reduced (P < 0.05) the inimical effects of phytic acid on endogenous amino acid flow at all dietary phytic acid levels. The composition of endogenous protein was also influenced (P < 0.10-0.001) by increasing phytic acid concentrations and phytase addition. The effects of phytic acid and phytase on endogenous flow and composition of endogenous protein, however, varied depending on the amino acid. It is concluded that the effects of phytase on amino acid digestibility may be mediated, in part, through a route of reduced endogenous loss.

  14. Additive effects of lupin protein and phytic acid on aortic calcification in ApoE deficient mice

    Directory of Open Access Journals (Sweden)

    Alexandra Schutkowski

    2015-03-01

    A two-factorial study with ApoE knockout mice was conducted in which mice received lupin protein isolate or casein with or without phytase. Phytic acid was added to the casein diets to a final concentration identical to the lupin protein diets. Here we show that the serum concentrations of cholesterol, lathosterol and desmosterol were lower and the faecal bile acid excretion was higher in the groups fed lupin proteins than in the groups fed casein (p < 0.05. Mice that received the lupin protein diet containing phytic acid were characterized by a lower aortic calcification than mice of the other three groups (p < 0.05. In conclusion, our results show that the cholesterol lowering properties of lupin protein isolate were not caused by phytic acid. However, the hypocalcific action of lupin proteins appears to depend on the combination of lupin proteins and phytic acid.

  15. Phytic acid in green leaves of herbaceous plants-temporal variation in situ and response to different nitrogen/phosphorus fertilizing regimes.

    Science.gov (United States)

    Alkarawi, Hassan Hadi; Zotz, Gerhard

    2014-08-13

    Phytic acid is the major storage compound for phosphorus (P) in plants. While accounting for up to 90 % in many seeds, usually only phytic acid in green leaves. This study follows up on the findings of a recent review of the occurrence of phytic acid in green leaves which revealed that (i) the current knowledge of phytic acid in leaves is mostly based on data from (fertilized) crop plants and (ii) the proportion of total P in phytic acid seems to decrease with improved P status in leaves in contrast to an increase in seeds and fruit. We studied five species of wild herbaceous plants in the field and under controlled conditions. Foliar P concentrations were much lower than those of the crops of earlier studies, but the proportion of P in phytic acid was similar, with little variation during the observation period. Both the field data and the experimental data showed a statistically indistinguishable negative correlation of phytic acid-P/total P and total P. In contrast to our expectation, this negative relationship was not related to differences in relative growth rates. We conclude that (i) our data of phytic acid concentrations in leaves of wild plants are in line with earlier observations on crops, and (ii) the trend towards lower proportions of phytic acid-P with increasing P status is probably a general phenomenon. Currently lacking a convincing explanation for the second observation, the role of phytic acid in foliar P metabolism is still unclear.

  16. Comparative Evaluation of Efficacy and Tolerability of Glycolic Acid, Salicylic Mandelic Acid, and Phytic Acid Combination Peels in Melasma.

    Science.gov (United States)

    Sarkar, Rashmi; Garg, Vijay; Bansal, Shivani; Sethi, Sumit; Gupta, Chitra

    2016-03-01

    Melasma is acquired symmetric hypermelanosis characterized by light-to-deep brown pigmentation over cheeks, forehead, upper lip, and nose. Treatment of this condition is difficult and associated with high recurrence rates. Chemical peels have become a popular modality in the treatment of melasma. To compare the therapeutic efficacy and tolerability of glycolic acid (35%) versus salicylic-mandelic (SM) acid (20% salicylic/10% mandelic acid) versus phytic combination peels in Indian patients with melasma. Ninety patients diagnosed with melasma were randomly assigned into 3 groups of 30 patients each. Group A received glycolic acid (GA-35%) peel, Group B received SM acid, and Group C received phytic combination peels. Each group was primed with 4% hydroquinone and 0.05% tretinoin cream for 4 weeks before treatment. Chemical peeling was done after every 14 days in all groups until 12 weeks. Clinical evaluation using melasma area and severity index (MASI) score and photography was recorded at every visit and follow-up was done until 20 weeks. There was a decrease in MASI score in all 3 groups but it was statistically significantly lower in Group A than Group C (p = .00), and it was also statistically significantly lower in Group B than Group C (p = .00) but there was no statistically significant difference between Groups A and B (p = .876). Objective response to treatment evaluated by reduction in MASI scoring after 12 weeks was 62.36% reduction in GA group, 60.98% reduction in SM group, and 44.71% in phytic acid group. It is concluded that GA (35%) and SM acid peels are both equally efficacious and a safe treatment modality for melasma in Indian skin, and are more effective than phytic acid peels. Salicylic-mandelic peels are better tolerated and more suitable for Indian skin.

  17. Fast determination of bioactive phytic acid and pyrophosphate in walnuts using microwave accelerated extraction.

    Science.gov (United States)

    Liu, Tong; He, Liu; Valiente, Manuel; López-Mesas, Montserrat

    2017-04-15

    Bioactive compounds phytic acid (IP6) and pyrophosphate (PPi) are minor components of walnuts with the ability of being inhibitors of urolithiasis, among others. Since simultaneous analysis of IP6 and PPi have known drawbacks, a new method to determine their content in walnuts has been developed with emphasis on their extraction from walnuts by microwave-assisted extraction (MAE). Acid content of extracting solvent, extraction time and temperature were optimized. After extraction, compounds were purified by selective adsorption/desorption on an anion exchange solid phase extraction and analyzed by inductive coupled plasma/mass spectrometry. A mixture of H2SO4 and HCl as solvent to extract both, IP6 and PPi, provided results slightly higher than those determined by conventional extraction with no statistical difference. The possible hydrolysis of phytic acid by MAE was analyzed. Compared with the conventional acid extraction method, significant improvement is achieved by the MAE method reducing extraction time from 3h to 10min.

  18. Genotype × Environment interaction for antioxidants and phytic acid contents in bread and durum wheat as influenced by climate

    National Research Council Canada - National Science Library

    Gordana Brankovic; Vesna Dragicevic; Dejan Dodig; Miroslav Zoric; Desimir Knezevic; Sladana Zilic; Srbislav Dencic; Gordana Surlan

    2015-01-01

    .... Fifteen bread wheat (Triticum aestivum L.) and 15 durum wheat (Triticum durum Desf.) genotypes were evaluated across six environments to determine contents of phytic acid (PA), inorganic P (P^sub...

  19. Efficacy of phytic acid as an inhibitor of enzymatic and non-enzymatic browning in apple juice.

    Science.gov (United States)

    Du, Yunjian; Dou, Siqi; Wu, Shengjun

    2012-11-15

    Browning decreases the commercial value of apple juice, and therefore colour preservation during processing and storage is the main objective of manufacturers. In this study, the efficacy of phytic acid as a browning inhibitor for use on apple juice was investigated. Browning of apple juice treated with phytic acid was monitored during processing and storage. 0.1 mM Phytic acid inhibited the polyphenol oxidase (PPO) from the apple juice by 99.2%. Consequently, the apple juice treated with phytic acid had significantly lower browning formation during processing and after 6 months of storage at room temperature compared with the control (p<0.05). Results indicate that this is a promising way to inhibit browning in apple juice.

  20. Reduction in phytic acid content and enhancement of antioxidant properties of nutricereals by processing for developing a fermented baby food

    National Research Council Canada - National Science Library

    Rasane, Prasad; Jha, Alok; Kumar, Arvind; Sharma, Nitya

    2015-01-01

    ...), phytic acid content (PAC) and free radical scavenging activity (FRSA). A formulation with the highest TPC, FRSA and the lowest PAC was selected to optimize a nutricereal based fermented baby food containing selected fermented cereal blends (FCB...

  1. Anticarcinogenic efficacy of phytic acid extracted from rice bran on azoxymethane-induced colon carcinogenesis in rats.

    Science.gov (United States)

    Norazalina, S; Norhaizan, M E; Hairuszah, I; Norashareena, M S

    2010-05-01

    This study is carried out to determine the potential of phytic acid extracted from rice bran in the suppression of colon carcinogenesis induced by azoxymethane (AOM) in rats. Seventy-two male Sprague-Dawley rats were divided into 6 groups with 12 rats in each group. The intended rats for cancer treatment received two intraperitoneal injections of AOM in saline (15mg/kg bodyweight) over a 2-week period. The treatments of phytic acid were given in two concentrations: 0.2% (w/v) and 0.5% (w/v) during the post-initiation phase of carcinogenesis phase via drinking water. The colons of the animals were analyzed for detection and quantification of aberrant crypt foci (ACF) after 8 weeks of treatment. The finding showed treatment with 0.2% (w/v) extract phytic acid (EPA) gave the greatest reduction in the formation of ACF. In addition, phytic acid significantly suppressed the number of ACF in the distal, middle and proximal colon as compared to AOM alone (pphytic acid (CPA) had the highest percentage (71%) of non-dysplastic ACF followed by treatment with 0.2% (w/v) EPA (61%). Administration of phytic acid also reduced the incidence and multiplicity of total tumors even though there were no significant differences between groups. In conclusion, this study found the potential value of phytic acid extracted from rice bran in reducing colon cancer risk in rats.

  2. Effect of processing conditions on phytic acid, calcium, iron, and zinc contents of lime-cooked maize.

    Science.gov (United States)

    Bressani, Ricardo; Turcios, Juan Carlos; Colmenares de Ruiz, Ana Silvia; de Palomo, Patricia Palocios

    2004-03-10

    Tortillas are made by cooking maize in a lime solution during variable times and temperatures, steeping the grain for up to 12 h, washing and grinding it to a fine dough, and cooking portions as flat cakes for up to 6 min. The effects of the main processing steps on the chemical composition, nutritive value, and functional and physicochemical characteristics have been areas of research. The present work evaluates the effect of lime concentration (0, 1.2, 2.4, and 3.6%) and cooking times (45, 60, and 75 min) on phytic acid retention of whole maize, its endosperm, and germ, as well as on the content of calcium, iron, and zinc on the same samples. The effects of steeping time and temperature and steeping medium on the phytic acid of lime-cooked maize were also studied. Finally, phytic acid changes from raw maize to tortilla were also measured. The results indicated that lime concentration and cooking time reduce phytic acid content in whole grain (17.4%), in endosperm (45.8%), and in germ (17.0%). Statistical analyses suggested higher phytic acid loss with 1.2% lime and 75 min of cooking. Cooking with the lime solution is more effective in reducing phytic acid than cooking with water. Steeping maize in lime solution at 50 degrees C during 8 h reduced phytic acid an additional 8%. The total loss of phytic acid from maize to tortilla was 22%. Calcium content increased in whole maize, endosperm, and germ with lime concentration and cooking and steeping times. The increase was higher in the germ than in the endosperm. The level, however, can be controlled if steeping of the cooked grain is conducted in water. Iron and zinc contents were not affected by nixtamalization processing variables but were affected in steeping.

  3. 低植酸作物育种研究进展%Research Advances of Low Phytic Acid Crop Breeding

    Institute of Scientific and Technical Information of China (English)

    郭怡璠; 杨淑萍; 张宏纪; 刘文林; 孙岩; 刘东军; 耿宏伟

    2015-01-01

    植酸广泛存在于作物种子中,由于植酸中的磷不能被非反刍动物有效吸收利用,而从粪便排出造成了磷资源的极大浪费和环境污染。近年来,利用理化诱变与转基因技术已成功地获得了玉米、大麦、水稻和大豆等作物的低植酸突变体。为进一步研究低植酸作物育种的现状,对植酸的简史、生物合成过程、低植酸突变体的培育及遗传特征、低植酸突变体农艺性状进行了综述,并对低植酸作物的应用前景进行了简要分析。%Phytic acid widely exists in crop seed ,phosphorus in phytic acid cannot be effectively utilized by non‐ruminant animals ,and from the waste discharge the waste caused by phosphorus resources ,or even cause envi‐ronmental pollution .In recent years ,the use of physical and chemical mutagenesis and transgenic technology had been successfully obtained corn ,barley ,rice and soybean crops such as low phytic acid mutants .In order to further research the low phytic acid plant breeding ,a brief history of phytic acid ,biosynthesis and low phytic acid mutant breeding and genetic characteristics ,low phytic acid mutant agronomic traits were reviewed ,and the application prospect of low phytic acid plants were analyzed in brief .

  4. Phytic acid suppresses ischemia-induced hydroxyl radical generation in rat myocardium.

    Science.gov (United States)

    Obata, Toshio; Nakashima, Michiko

    2016-03-05

    The present study examined whether ischemia-reperfusion-induced hydroxyl radical (·OH) generation was attenuated by myo-inositol hexaphosphoric acid (phytic acid). A flexibly mounted microdialysis technique was used to detect the generation of ·OH in in vivo rat hearts. To measure the level of ·OH, sodium salicylate in Ringer's solution (0.5mM or 0.5 nmol/μl/min) was infused directly through a microdialysis probe to detect the generation of ·OH as reflected by the nonenzymatic formation of 2,3-dihydroxybenzoic acid (2,3-DHBA). To confirm the generation of ·OH by Fenton-type reaction, iron(II) was infused through a microdialysis probe. A positive linear correlation between iron(II) and the formation of 2,3-DHBA (R(2)=0.983) was observed. However, the level of 2,3-DHBA in norepinephrine (100 μM) plus phytic acid (100 μM) treated group were significantly lower than those observed in norepinephrine-only-treated group (n=6, *pphytic acid on ischemia-reperfusion-induced ·OH generation, the heart was subjected to myocardial ischemia for 15 min by occlusion of the left anterior descending coronary artery (LAD). When the heart was reperfused, the normal elevation of 2,3-DHBA in the heart dialysate was not observed in animals pretreated with phytic acid. These results suggest that phytic acid is associated with antioxidant effect due to the suppression of iron-induced ·OH generation.

  5. Porous Zirconium-Phytic Acid Hybrid: a Highly Efficient Catalyst for Meerwein-Ponndorf-Verley Reductions.

    Science.gov (United States)

    Song, Jinliang; Zhou, Baowen; Zhou, Huacong; Wu, Lingqiao; Meng, Qinglei; Liu, Zhimin; Han, Buxing

    2015-08-03

    The utilization of compounds from natural sources to prepare functional materials is of great importance. Herein, we describe for the first time the preparation of organic-inorganic hybrid catalysts by using natural phytic acid as building block. Zirconium phosphonate (Zr-PhyA) was synthesized by reaction of phytic acid and ZrCl4 and was obtained as a mesoporous material with pore sizes centered around 8.5 nm. Zr-PhyA was used to catalyze the mild and selective Meerwein-Ponndorf-Verley (MPV) reduction of various carbonyl compounds, e.g., of levulinic acid and its esters into γ-valerolactone. Further studies indicated that both Zr and phosphate groups contribute significantly to the excellent performance of Zr-PhyA.

  6. Phytic acid mobilization is an early response to chilling of the embryonic axes from dormant oilseed of hazel (Corylus avellana).

    Science.gov (United States)

    Andriotis, Vasilios M E; Smith, Susan B; Ross, James D

    2005-02-01

    Dormancy of hazel (Corylus avellana L.) seeds is alleviated by a chilling treatment during which cytological, hormonal, and biochemical changes occur. Phytic acid and phosphate mobilization have been examined during this treatment. Phytic acid accounted for 0.7% and up to 3.2% of dry weight in axiferous and cotyledonary tissue, respectively. Phytic acid levels in embryonic axes were reduced by 60% within the first 3 weeks of chilling, with little subsequent change, in contrast to warm-imbibed tissue where levels did not change significantly. In cotyledons, phytic acid was mobilized to a lesser extent. Phosphate levels expressed on a fresh weight basis remained almost unaltered suggesting either the operation of a homeostatic mechanism for intracellular concentration or rapid utilization due to active metabolism. Phytase activity increased during stratification in both axiferous and cotyledonary tissue. The initial rise observed was associated with dormancy alleviation, since it occurred before the realization of full germination potential by the seeds and not in warm-imbibed tissue. Protein bodies were isolated from hazel seeds by non-aqueous density gradients. Phytase activity was closely associated with the purified organelles, where phytic acid was located by light microscopy. Overall, these findings suggest that phytic acid mobilization by phytase and previously described processes associated with protein bodies, such as considerable proteolysis, are early participants in the plethora of events leading to seed dormancy relief and germination in hazel.

  7. Interactive suppression of aberrant crypt foci induced by azoxymethane in rat colon by phytic acid and green tea.

    Science.gov (United States)

    Challa, A; Rao, D R; Reddy, B S

    1997-10-01

    Several epidemiological studies point to a strong correlation between nutrient composition of the diet and cancer of the colon. Phytic acid, present in grains, has been credited with reducing the risk of cancer of the colon. A number of reports are available indicating the benefits of green tea consumption in reducing the risk of stomach, lung and skin cancer, but little data are available on the effect of green tea in reducing the risk of colon cancer. Also, there are no studies on the combined effect of these compounds on colon tumorigenesis. Thus the primary objective of this investigation was to elucidate the combined effects of green tea and phytic acid on colonic preneoplastic lesions and the Phase II enzyme glutathione S-transferase. Fisher 344 male weanling rats were divided into nine groups of 15 rats each and fed the experimental diet for 13 weeks. Rats received two s.c. injections of azoxymethane in saline at 16 mg/kg body wt at 7 and 8 weeks of age. Rats received three levels (0, 1 and 2%) of phytic acid with three levels (0, 1 and 2%) of green tea within each phytic acid level in a 3 x 3 factorial experiment. Results indicate that while green tea had a marginal effect (P green tea and phytic acid was significant (P green tea and phytic acid.

  8. Thermal Behavior and Free-Radical-Scavenging Activity of Phytic Acid Alone and Incorporated in Cosmetic Emulsions

    Directory of Open Access Journals (Sweden)

    André Luis Máximo Daneluti

    2015-07-01

    Full Text Available Phytic acid is a natural compound widely used as depigmenting agent in cosmetic emulsions. Few studies are available in the literature covering the stability and the antioxidating property of this substance, used alone or into emulsions. Therefore, the purpose of this work was to investigate the thermal behavior and antioxidant properties of phytic acid alone and into cosmetic emulsions. The thermal behavior of this substance was evaluated by thermogravimetry (TG/derivative thermogravimetry (DTG and differential scanning calorimetry (DSC and the free-radical-scavenging activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH. TG/DTG and DSC curves allowed evaluation of the thermal behavior of phytic acid. These results showed that the substance presented four stages of mass loss. Thermal decomposition of the material initiated at 150 °C. Thermal behavior of the cosmetic emulsions detected that the addition of phytic acid decreased the thermal stability of the system. DPPH free-radical-scavenging activity showed that phytic acid incorporated into emulsion had no antioxidant capacity compared to BHT. In summary, we concluded that the thermoanalytical techniques (TG and DSC were efficient and reliable in the characterization of phytic acid alone and incorporated into cosmetic emulsions.

  9. Effect of phytic acid used as etchant on bond strength, smear layer, and pulpal cells.

    Science.gov (United States)

    Nassar, Mohannad; Hiraishi, Noriko; Islam, Md Sofiqul; Aizawa, Mamoru; Tamura, Yukihiko; Otsuki, Masayuki; Kasugai, Shohei; Ohya, Keiichi; Tagami, Junji

    2013-10-01

    This study aimed to evaluate the effect of phytic acid (IP6), used as etchant, on resin-dentin bond strength, smear layer removal, and the viability of pulpal cells. Flat dentin surfaces with smear layer were etched with 1% IP6 for 60, 30, or 15 s; in the control group 37% phosphoric acid (PA) was used. Dentin surfaces were rinsed, blot-dried, and bonded with an etch-and-rinse adhesive, followed by composite build-ups. The specimens were subjected to tensile testing after 24 h of water storage at 37°C, and failure modes were determined using scanning electron microscopy. The effectiveness of IP6 to remove the smear layer was observed using scanning electron microscopy. To evaluate the effect on pulpal cells, solutions of 0.1 and 0.01% IP6 and of 3.7 and 0.37% PA were prepared and rat pulpal cells were treated with these solutions for 6 and 24 h. Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results demonstrated that all application times of IP6 produced bond-strength values that were significantly higher than that of the control. Phytic acid effectively removed the smear layer and plugs, thus exposing the collagen network. Phytic acid had a minimal effect on pulpal cells, whereas PA resulted in a marked decrease in their viability.

  10. Phytic acid doped polyaniline containing epoxy coatings for corrosion protection of Q235 carbon steel

    Science.gov (United States)

    Hao, Yongsheng; Sani, Luqman Abdullahi; Ge, Tiejun; Fang, Qinghong

    2017-10-01

    Corrosion protection of epoxy coatings contained with phytic acid-doped polyaniline (PANI-PA) for Q235 carbon steel was studied in this work. Synthesized PANI-PA particles were characterized by XPS, TGA, and FTIR, respectively. The coating performance was investigated by OCP, EIS, and SVET, respectively. The experimental results show that the concentration of PANI-PA has a significant influence to the barrier effect of the epoxy coating. Epoxy coating loaded with 2 wt.% PANI-PA has the best protection ability and self-healing function to a certain degree. The self-healing function of PANI-PA is attributed to the synergistic effect of the passivation of PANI and the chelation of the dedoped phytic acid ions with iron ions. Therefore, PANI-PA can be used as an effective anticorrosion pigment in future.

  11. Effect of dietary phytic acid and inorganic iron on the quality of chilled pork.

    Directory of Open Access Journals (Sweden)

    Renilda Terezinha Monteiro

    2015-09-01

    Full Text Available The objective of this study was to evaluate the effect of supplementing inorganic iron and phytic acid to the diet for finishing pigs on meat quality after 24 hours and 7 days of refrigeration. Forty castrated male finishing pigs of a commercial genotype, with an initial mean weight of 64.34 ± 6.64 kg and age of 108 days, were used. The animals were weighed and housed individually in brick pens with an area of three m2 and compact floor, receiving water and ration ad libitum for 30 days. A randomized block design in a 2 x 2 factorial scheme was used, corresponding to diets supplemented or not with inorganic iron and with two levels of phytic acid, high (4.85% and low (2.98%. The animals were slaughtered when they had reached a mean weight of 100.76 ± 6.54 kg and longissimus dorsi muscle samples were collected for the analysis of meat quality. The following parameters were analyzed in the samples: pH, color, marbling, water loss through pressure, shear force, iron composition, and lipid oxidation. No differences in the variables analyzed were observed between factors, except for muscle iron concentration, which was higher for the diet with inclusion of inorganic iron. Lipid oxidation was not influenced by the presence or absence of phytic acid and inorganic iron. The results show that diets with elevated phytic acid levels supplemented or not with inorganic iron can be used for finishing pigs without compromising meat quality during the refrigeration phase.

  12. Influence of substrate composition on the formation of phytic acid conversion coatings

    Energy Technology Data Exchange (ETDEWEB)

    Cui, X.; Wang, F. [School of Material Science and Chemical Engineering, Harbin Engineering University, Harbin (China); State Key Laboratory for Corrosion and Protection, Institute of Metal Research, Chinese Academy of Sciences, Shenyang (China); Jin, G.; Liu, E.; Ding, M.; Li, Q. [School of Material Science and Chemical Engineering, Harbin Engineering University, Harbin (China)

    2012-03-15

    In this paper, the formation and corrosion resistance of the phytic acid conversion coatings on Mg, Al, and AZ91D magnesium alloy were contrastively investigated using scanning electronic microscopy (SEM), Auger electron spectroscopy (AES), Fourier transform infrared spectroscopy (FTIR), electronic probe microscopic analyzer (EPMA), electronic balance, and electrochemical methods. The influence of phytic acid conversion coating as a middle layer on the properties of the paint on magnesium alloys was also investigated. The results show that the formation process of the conversion coatings is evidently influenced by the compositions of the substrate. The coating on pure aluminum is thinner and compacter than that on pure magnesium and the coating formed on {alpha} phase in AZ91D magnesium alloy is thinner but denser than that on {beta} phase. The phytic acid conversion coatings formed on Mg, Al, and AZ91D magnesium alloy can all increase their corrosion resistance. The active functional groups of hydroxyl and phosphate radical are rich in the conversion coatings, which can improve the bonding between the organic paint and magnesium alloy and then improve their corrosion resistance. (Copyright copyright 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  13. In vitro corrosion and biocompatibility study of phytic acid modified WE43 magnesium alloy

    Science.gov (United States)

    Ye, C. H.; Zheng, Y. F.; Wang, S. Q.; Xi, T. F.; Li, Y. D.

    2012-02-01

    Phytic acid (PA) conversion coating on WE43 magnesium alloy was prepared by the method of immersion. The influences of phytic acid solution with different pH on the microstructure, properties of the conversion coating and the corrosion resistance were investigated by SEM, FTIR and potentiodynamic polarization method. Furthermore, the biocompatibility of different pH phytic acid solution modified WE43 magnesium alloys was evaluated by MTT and hemolysis test. The results show that PA can enhance the corrosion resistance of WE43 magnesium especially when the pH value of modified solution is 5 and the cytotoxicity of the PA coated WE43 magnesium alloy is much better than that of the bare WE43 magnesium alloy. Moreover, all the hemolysis rates of the PA coated WE43 Mg alloy were lower than 5%, indicating that the modified Mg alloy met the hemolysis standard of biomaterials. Therefore, PA coating is a good candidate to improve the biocompatibility of WE43 magnesium alloy.

  14. Seed Biofortification and Phytic Acid Reduction: A Conflict of Interest for the Plant?

    Directory of Open Access Journals (Sweden)

    Francesca Sparvoli

    2015-11-01

    Full Text Available Most of the phosphorus in seeds is accumulated in the form of phytic acid (myo-inositol-1,2,3,4,5,6-hexakisphosphate, InsP6. This molecule is a strong chelator of cations important for nutrition, such as iron, zinc, magnesium, and calcium. For this reason, InsP6 is considered an antinutritional factor. In recent years, efforts to biofortify seeds through the generation of low phytic acid (lpa mutants have been noteworthy. Moreover, genes involved in the biosynthesis and accumulation of this molecule have been isolated and characterized in different species. Beyond its role in phosphorus storage, phytic acid is a very important signaling molecule involved in different regulatory processes during plant development and responses to different stimuli. Consequently, many lpa mutants show different negative pleitotropic effects. The strength of these pleiotropic effects depends on the specific mutated gene, possible functional redundancy, the nature of the mutation, and the spatio-temporal expression of the gene. Breeding programs or transgenic approaches aimed at development of new lpa mutants must take into consideration these different aspects in order to maximize the utility of these mutants.

  15. Evaluation of phytic acid utilization by S. cerevisiae strains used in fermentation processes and biomass production.

    Science.gov (United States)

    Mikulski, Dawid; Kłosowski, Grzegorz

    2017-01-01

    Saccharomyces cerevisiae is a well-studied yeast species used mainly in fermentation processes, bakery, and for SCP (Single Cell Protein) acquisition. The aim of the study was to analyze the possibility of phytic acid utilization as one of the hydrolysis processes carried out by yeast. The analysis of 30 yeast strains used in fermentation and for biomass production, that were grown in media containing phytic acid, revealed a high variability in the biomass production rate and the capability to hydrolyze phytates. No correlation between a high biomass concentration and a high level of phytate hydrolysis was found. Only four analyzed strains (Bayanus IOC Efficience, Sano, PINK EXCEL, FINAROME) were able to reduce the phytic acid concentration by more than 33.5%, from the initial concentration 103.0 ± 2.1 μg/ml to the level below 70 μg/ml. The presented results suggest that the selected wine and fodder yeast can be used as in situ source of phosphohydrolases in fermentation processes, and especially in the production of fodder proteins. However, further studies aimed at the optimization of growing parameters, such as the maximization of phytase secretion, and a comprehensive analysis of the catalytic activity of the isolated phosphohydrolases, are necessary.

  16. Marker assisted selection of low phytic acid trait in maize (Zea mays L.).

    Science.gov (United States)

    Sureshkumar, S; Tamilkumar, P; Senthil, N; Nagarajan, P; Thangavelu, A U; Raveendran, M; Vellaikumar, S; Ganesan, K N; Balagopal, R; Vijayalakshmi, G; Shobana, V

    2014-02-01

    Maize is the third important major food crop. Breeding for low phytate maize genotypes is an effective strategy for decreasing the content of kernel phytic acid (a chelator of cations such as Ca(2+) and Fe(3+) ) and thereby increasing the bioavailability of nutritive minerals in human diet and animal feed. Previous studies have established that a mutant plant with a lpa2-2 allele accumulates less phytic acid in seeds. Therefore, the marker assisted backcross breeding (MABB), which involves introgression of lpa2-2 recessive allele (which confer low phytate trait) from a lpa2-2 mutant line into a well-adapted line using backcrosses and selection of lines possessing lpa2-2 allele in each backcross population using molecular markers, is an effective strategy for developing low phytate maize. So far, no studies have developed any lpa2-2 allele specific molecular markers for this purpose. Here, using backcross and selfed progenies, obtained by crossing low phytate mutant line 'EC 659418' (i.e. donor of lpa2-2 allele) into agronomically superior line 'UMI395', we have validated that a SSR marker 'umc2230', located 0.4 cM downstream of lpa2-2, cosegregate, in a Mendelian fashion, with low phytic acid trait. Therefore umc2230 can be dependably used in MABB for the development of low phytate maize.

  17. Expression of D-myo-inositol-3-phosphate synthase in soybean. Implications for phytic acid biosynthesis.

    Science.gov (United States)

    Hegeman, C E; Good, L L; Grabau, E A

    2001-04-01

    Phytic acid, a phosphorylated derivative of myo-inositol, functions as the major storage form of phosphorus in plant seeds. Myo-inositol phosphates, including phytic acid, play diverse roles in plants as signal transduction molecules, osmoprotectants, and cell wall constituents. D-myo-inositol-3-phosphate synthase (MIPS EC 5.5.1.4) catalyzes the first step in de novo synthesis of myo-inositol. A soybean (Glycine max) MIPS cDNA (GmMIPS1) was isolated by reverse transcriptase-PCR using consensus primers designed from highly conserved regions in other plant MIPS sequences. Southern-blot analysis and database searches indicated the presence of at least four MIPS genes in the soybean genome. Northern-blot and immunoblot analyses indicated higher MIPS expression and accumulation in immature seeds than in other soybean tissues. MIPS was expressed early in the cotyledonary stage of seed development. The GmMIPS1 expression pattern suggested that it encodes a MIPS isoform that functions in seeds to generate D-myo-inositol-3-phosphate as a substrate for phytic acid biosynthesis.

  18. Effects of phytic acid and exercise on some serum analytes in rats orally exposed to diets supplemented with cadmium.

    Science.gov (United States)

    Daley, Tasha; Omoregie, Samson N; Wright, Vincent; Omoruyi, Felix O

    2013-03-01

    Cadmium is an environmental pollutant of increasing worldwide concern. It has been reported to be high in the soil where food crops are grown in some parishes of Jamaica. Surprisingly, no adverse effect of cadmium has been reported among the Jamaican population. However, phytic acid has also been shown to be high in some food crops grown in Jamaica. In this study, we evaluated the effects of phytic acid (1 %) and exercise on the metabolism of cadmium (5 mg cadmium/kg body weight) in rats. Five groups of rats were fed as follows: rats fed control diet, control diet supplemented with cadmium and subjected to exercise, control diet supplemented with phytic acid plus cadmium and subjected to exercise, control diet supplemented with cadmium plus phytic acid, and control diet supplemented with cadmium only. The animals were fed for 4 weeks and then sacrificed. Blood samples were collected for some biochemical assays. Percentage weight loss (28.42 %) was greatest in the group that had cadmium supplement only. The group fed control diet supplemented with cadmium only displayed increased liver enzymes and electrolytes except for the significant decrease in bicarbonate compared to other test groups. Similarly, blood urea nitrogen and uric acid were increased in the group fed cadmium supplement only compared to other test groups. Total cholesterol trended downwards in the test groups compared to control. These observations suggest that consumption of diet high in phytic acid with relatively high physical activity may be protective against the adverse effects of cadmium.

  19. Multi-walled carbon nanotubes in aqueous phytic acid for enhancing biosensor

    Science.gov (United States)

    Guo, Xiaoyu; Miao, Yun; Ye, Pingping; Wen, Ying; Yang, Haifeng

    2014-04-01

    The poor dispersion of carbon based nanomaterials without strong acid pretreatment in aqueous solution is a fundamental problem, limiting its applications in biology-related fields. A good dispersion of multi-walled carbon nanotubes (MWCNTs) in water was realized by 50 wt.% phytic acid (PA) solution. As an application case, the PA-MWCNTs dispersion in aqueous solution was used for the immobilization of horseradish peroxidase (HRP) and its direct electrochemistry was realized. The constructed biosensor has a sound limit of detection, wide linear range, and high affinity for hydrogen peroxide (H2O2) as well as being free from interference of co-existing electro-active species.

  20. Screening, selection and characterization of phytic acid degrading lactic acid bacteria from chicken intestine.

    Science.gov (United States)

    Raghavendra, Ponnala; Halami, Prakash M

    2009-07-31

    This study was undertaken to screen and select potent phytate degrading lactic acid bacteria and to evaluate their additional characteristic features. Forty lactic acid bacterial strains were isolated from different sources and screened for their ability to degrade myo-inositol hexaphosphate or IP(6) by cobalt chloride staining (plate assay) method, using calcium or sodium salt of phytic acid as substrate. All the forty isolates were able to degrade calcium phytate. However, only two Pediococcus pentosaceus strains (CFR R38 and CFR R35) were found to degrade sodium phytate. These strains showed phytase activity of 213 and 89 U at 50 degrees C, respectively and poor acid phosphatase activity. These strains were further evaluated for additional characteristic features. At pH 2, P. pentosaceus strains CFR R38 and CFR R35 showed 50.7 and 48.5 percentage survivability after 2 h of incubation respectively and they could also withstand 0.3% ox-bile. These cultures exhibited 54.6 and 44.8% of hydrophobicity to xylene, antibacterial activity against food borne pathogens and possessed beta-galactosidase activity. The resistance pattern to several antibiotics was also analyzed. The present study indicates that these strains, having phytate degrading ability and other characteristic features can be exploited as starter cultures in fermented foods to improve the mineral bioavailability.

  1. Antioxidant capacity of phytic acid purified from rice bran - doi: 10.4025/actascitechnol.v34i4.16358

    Directory of Open Access Journals (Sweden)

    Cristiane Canan

    2012-10-01

    Full Text Available Rice bran is a by-product of rice processing industry, with high levels of phytic acid or phytate. Considering phytic acid antioxidant activity, its various applications and its high concentration in rice bran, this study had the objective of evaluating the antioxidant capacity of purified phytic acid from rice bran using three different methods. Using of 2,4,6-tripyridil-s-triazine or method of FRAP (Ferric Reducing Antioxidant Power, reducing Fe2+ activity was not detected for standard or purified phytic acid. With BPS (bathophenanthroline method, the Fe2+ chelator activity of standard phytic acid and rice bran phytic acid were dependent on the concentration and contact time and were observed IC50 values of 4.39 mg mL-1 and IC50 of 7.54 mg mL-1, respectively. By the deoxyribose method, the standard phytic acid inhibited the hydroxyl radical with an IC50 of 0.70 mg mL-1 while the rice bran phytic acid showed a maximum inhibitory activity of 40% associated to its chelating capacity and confirm this important antioxidant capacity.

  2. Identification of genes necessary for wild-type levels of seed phytic acid in Arabidopsis thaliana using a reverse genetics approach

    Science.gov (United States)

    The majority of phosphorus (P) in seeds is found in the form of phytic acid (InsP6) which 22 accumulates as the mixed salt phytate and accounts for > 60% of total seed P. InsP6 is generally 23 considered to be an anti-nutrient and the development of low phytic acid (lpa) seed crops is of 24 signific...

  3. Rice antioxidants: phenolic acids, flavonoids, anthocyanins, proanthocyanidins, tocopherols, tocotrienols, γ-oryzanol, and phytic acid.

    Science.gov (United States)

    Goufo, Piebiep; Trindade, Henrique

    2014-03-01

    Epidemiological studies suggested that the low incidence of certain chronic diseases in rice-consuming regions of the world might be associated with the antioxidant compound contents of rice. The molecules with antioxidant activity contained in rice include phenolic acids, flavonoids, anthocyanins, proanthocyanidins, tocopherols, tocotrienols, γ-oryzanol, and phytic acid. This review provides information on the contents of these compounds in rice using a food composition database built from compiling data from 316 papers. The database provides access to information that would have otherwise remained hidden in the literature. For example, among the four types of rice ranked by color, black rice varieties emerged as those exhibiting the highest antioxidant activities, followed by purple, red, and brown rice varieties. Furthermore, insoluble compounds appear to constitute the major fraction of phenolic acids and proanthocyanidins in rice, but not of flavonoids and anthocyanins. It is clear that to maximize the intake of antioxidant compounds, rice should be preferentially consumed in the form of bran or as whole grain. With respect to breeding, japonica rice varieties were found to be richer in antioxidant compounds compared with indica rice varieties. Overall, rice grain fractions appear to be rich sources of antioxidant compounds. However, on a whole grain basis and with the exception of γ-oryzanol and anthocyanins, the contents of antioxidants in other cereals appear to be higher than those in rice.

  4. Preventive effect of phytic acid on lysosomal hydrolases in normal and isoproterenol-induced myocardial infarction in Wistar rats.

    Science.gov (United States)

    Brindha, E; Rajasekapandiyan, M

    2015-02-01

    This study was aimed to evaluate the preventive role of phytic acid on lysosomal enzymes in isoproterenol (ISO)-induced myocardial infarction (MI) in male Wistar rats. Rats subcutaneously injected with ISO (85 mg/kg) at an interval of 24 h for two days showed a significant increase in the activities of lysosomal enzymes (glucuronidase, N-acetyl glucosaminidase, galactosidase, cathepsin-B and cathepsin-D) were increased significantly in serum and the heart of ISO-induced rats, but the activities of glucuronidase and cathepsin-D were decreased significantly in the lysosomal fraction of the heart. Pretreatment with phytic acid (25 and 50 mg/kg) daily for a period of 56 d positively altered activities of lysosomal hydrolases in ISO-induced rats. Thus, phytic acid possesses a cardioprotective effect in ISO-induced MI in rats.

  5. Optimization of the treatment of wheat samples for the determination of phytic acid by HPLC with refractive index detection.

    Science.gov (United States)

    Amaro, Rosa; Murillo, Miguel; González, Zurima; Escalona, Andrés; Hernández, Luís

    2009-01-01

    The treatment of wheat samples was optimized before the determination of phytic acid by high-performance liquid chromatography with refractive index detection. Drying by lyophilization and oven drying were studied; drying by lyophilization gave better results, confirming that this step is critical in preventing significant loss of analyte. In the extraction step, washing of the residue and collection of this water before retention of the phytates in the NH2 Sep-Pak cartridge were important. The retention of phytates in the NH2 Sep-Pak cartridge and elimination of the HCI did not produce significant loss (P = 0.05) in the phytic acid content of the sample. Recoveries of phytic acid averaged 91%, which is a substantial improvement with respect to values reported by others using this methodology.

  6. Antioxidant and type 2 diabetes related functional properties of phytic acid extract from Kenyan local food ingredients: effects of traditional processing methods.

    Science.gov (United States)

    Kunyanga, Catherine N; Imungi, Jasper K; Okoth, Michael W; Biesalski, Hans K; Vadivel, Vellingiri

    2011-01-01

    Emerging scientific evidences reveal that phytic acid has several positive effects on human health. The antioxidant and type 2 diabetes related enzyme inhibition properties of phytic acid extract prepared from raw and traditionally processed local grains and vegetables collected from Kenya were evaluated. Phytic acid content of raw grains and vegetables ranged between 2.81-3.01 and 0.29-3.23 g/100 g DM, respectively. The phytic acid extract from raw samples revealed 59%-89% of DPPH radical scavenging capacity, 27-3,526 mmol Fe(II)/g extract of reducing power, 20%-72% of α-amylase inhibition activity and 8%-91% of α-glucosidase inhibition activity. Cooking and roasting improved the antioxidant and health relevant functionality of phytic acid extracts obtained from Kenyan local vegetables and grains, respectively.

  7. Genetic variation for phytic acid content in mungbean(Vigna radiata L. Wilczek)

    Institute of Scientific and Technical Information of China (English)

    Vinod; Janardan; Dhole; Kandali; Srinivasalu; Reddy

    2015-01-01

    Mungbean(Vigna radiata L. Wilczek) is a short-duration legume crop cultivated for seeds that are rich in protein and carbohydrates. Mungbeans contain phytic acid(PA), an anti-nutritional factor that is the main storage form of organic phosphorus in seeds. It is a strong inhibitor against the absorption of nutrients including iron, zinc, calcium and magnesium in monogastric animals. Genotypes with low phytic acid(lpa) in seed may show increased assimilation of nutrients and be useful in breeding lpa cultivars. The present study was conducted to identify lpa sources, genetic variation, heritability, and association with seed coat color, inorganic phosphorus(IP), and seed size in 102 mungbean genotypes including released varieties, land races, mutants, and wild species grown in two seasons: summer 2011 and rabi 2012. PA and IP in dry seeds were estimated by modified colorimetric method and Chen’s modified method,respectively. PA, IP, and 100-seed weight differed significantly in the two seasons. PA content in102 genotypes ranged from 5.74 to 18.98 mg g-1and 5.85 to 20.02 mg g-1in summer 2011 and rabi 2012, respectively. High heritability was found for PA(0.87 and 0.86) and seed size(0.82 and0.83) but low heritability for IP(0.61 and 0.60). A negative correlation was found between PA and seed size(r =-0.183 and-0.267). Yellow and green seed coat genotypes contained significantly less PA than black seed coat genotypes. Cluster analysis revealed the distinctness of wild species, land races and cultivated varieties on the basis of PA content. The genotypes YBSM(6.001 mg g-1) and JL-781(6.179 mg g-1) showed lowest PA. These lpa sources can be used to develop high-yielding mungbean cultivars with low phytic acid.

  8. Metabolite profiling of two novel low phytic acid (lpa) soybean mutants.

    Science.gov (United States)

    Frank, Thomas; Nörenberg, Svenja; Engel, Karl-Heinz

    2009-07-22

    A GC-based approach was applied to compare the metabolite profiles of two low phytic acid (lpa) soybean mutants and their respective wild-types. The lpa mutants (Gm-lpa-TW75-1 and Gm-lpa-ZC-2) were grown together with the wild-types (Taiwan 75 and Zhechun no. 3) in three and four field trials, respectively. HPLC analysis revealed a phytic acid reduction of -53% for Gm-lpa-TW75-1 and of -46% for Gm-lpa-ZC-2. For Gm-lpa-TW75-1, no accumulation of lower inositol phosphates was observed, whereas Gm-lpa-ZC-2 exhibited significantly increased contents of the lower inositol phosphates InsP(3), InsP(4), and InsP(5) compared to the corresponding wild-type. The metabolite profiling revealed that compared to the wild-types, 40% (Gm-lpa-TW75-1) and 21% (Gm-lpa-ZC-2) of the detected peaks were statistically significantly different in the lpa mutants grown at one field trial. However, the majority of these differences were shown to be related to environmental impact and natural variability rather than to the mutation event. Identification of consistent metabolic changes in the lpa mutants revealed decreased contents of myo-inositol, galactinol, raffinose, stachyose, and the galactosyl cyclitols galactopinitol A, galactopinitol B, and fagopyritol B1 compared to the wild-type. These consistently pronounced changes in Gm-lpa-TW75-1 confirmed the suggested mutation target. Consideration of the metabolic changes observed for Gm-lpa-ZC-2 (accumulation of lower inositol phosphates and increased myo-inositol contents) indicated a mutation event affecting the latter biosynthetic steps leading to phytic acid. The study demonstrated the applicability of metabolite profiling for the detection of changes in the metabolite phenotype induced by mutation breeding and its power in assisting in the elucidation of mutation events.

  9. Genetic variation for phytic acid content in mungbean (Vigna radiata L. Wilczek

    Directory of Open Access Journals (Sweden)

    Vinod Janardan Dhole

    2015-03-01

    Full Text Available Mungbean (Vigna radiata L. Wilczek is a short-duration legume crop cultivated for seeds that are rich in protein and carbohydrates. Mungbeans contain phytic acid (PA, an anti-nutritional factor that is the main storage form of organic phosphorus in seeds. It is a strong inhibitor against the absorption of nutrients including iron, zinc, calcium and magnesium in monogastric animals. Genotypes with low phytic acid (lpa in seed may show increased assimilation of nutrients and be useful in breeding lpa cultivars. The present study was conducted to identify lpa sources, genetic variation, heritability, and association with seed coat color, inorganic phosphorus (IP, and seed size in 102 mungbean genotypes including released varieties, land races, mutants, and wild species grown in two seasons: summer 2011 and rabi 2012. PA and IP in dry seeds were estimated by modified colorimetric method and Chen's modified method, respectively. PA, IP, and 100-seed weight differed significantly in the two seasons. PA content in 102 genotypes ranged from 5.74 to 18.98 mg g− 1 and 5.85 to 20.02 mg g− 1 in summer 2011 and rabi 2012, respectively. High heritability was found for PA (0.87 and 0.86 and seed size (0.82 and 0.83 but low heritability for IP (0.61 and 0.60. A negative correlation was found between PA and seed size (r = − 0.183 and − 0.267. Yellow and green seed coat genotypes contained significantly less PA than black seed coat genotypes. Cluster analysis revealed the distinctness of wild species, land races and cultivated varieties on the basis of PA content. The genotypes YBSM (6.001 mg g− 1 and JL-781 (6.179 mg g− 1 showed lowest PA. These lpa sources can be used to develop high-yielding mungbean cultivars with low phytic acid.

  10. Solution of Azelaic Acid (20%), Resorcinol (10%) and Phytic Acid (6%) Versus Glycolic Acid (50%) Peeling Agent in the Treatment of Female Patients with Facial Melasma.

    Science.gov (United States)

    Faghihi, Gita; Taheri, Azam; Shahmoradi, Zabihollah; Nilforoushzadeh, Mohammad Ali

    2017-01-01

    Melasma, a common acquired disorder of hyperpigmentation, especially in women, is often resistant to therapy. This study was aimed to evaluate the efficacy and safety of azelaic acid, resorcinol and phytic acid solution in chemical peeling of melasma in comparison to 50% glycolic acid. This clinical trial was performed, on 42 female patients with bilateral melasma. Severity of melasma was assessed by melasma area and severity index (MASI). Combination of (20% azelaic acid + 10% resorcinol + 6% phytic acid) was used as a new peeling agent on the right side of the face and 50% glycolic acid on the left side every 2 weeks for 6 times. Follow-up was carried out for 3 months after the last session. Any decrease in MASI score and unwanted complications following peeling were evaluated and compared during the trial. Patients showed marked improvement as calculated with MASI score before and after treatment in both sides of the face. The efficacy of combination formula (azelaic acid, resorcinol and phytic acid) was similar to glycolic acid, but with fewer complications. There was no statistically difference in improvement between two groups (P > 0.05). However, the patient's discomfort following procedures was significantly lower with azelaic acid, resorcinol and phytic compared with the glycolic acid peels (P glycolic acid peel.

  11. Application of HPLC for determination of phytic acid in the colonic epithelial Caco-2 cells.

    Science.gov (United States)

    Weglarz, Ludmiła; Parfiniewicz, Beata; Bat, Beata; Orchel, Arkadiusz; Dzierzewicz, Zofia; Wilczok, Tadeusz

    2004-12-01

    Phytic acid (myo-inositol hexaphosphate, IP6) is currently receiving a considerable interest because of its anticancer (preventive and therapeutic) potential against colon tumors and the need for methods of its determination. The aim of this study was to analyze the uptake of IP6 by human colon adenocarcinoma cells (Caco-2 cell line) and to evaluate the method of its intracellular quantification with the use of high performance liquid chromatography (HPLC) technique. Chromatographic analysis revealed a rapid uptake of IP6 by cells. The intracellular accumulation of IP6 was saturable at 0.5 h and it did not change with the prolongation of incubation up to 72 h.

  12. Low-phytic acid barley improves calcium and phosphorus utilization and growth performance in growing pigs.

    Science.gov (United States)

    Veum, T L; Ledoux, D R; Bollinger, D W; Raboy, V; Cook, A

    2002-10-01

    Thirty-five crossbred barrows averaging 13.5 kg starting BW were used in a 35-d experiment to compare the availability of P and the nutritional value of two near-isogenic progeny of the barley cultivar 'Harrington'. Low-phytic acid barley (LPB, 0.35% total P, 0.14% phytic acid P) was homozygous for the low-phytic acid 1-1 allele, and the normal barley (NB, 0.35% total P, 0.24% phytic acid P) was homozygous for the normal allele of that gene. Pigs were fed individually twice daily in metabolism pens. Barley was the only source of phytate in semipurified diets, 1 to 3. Diet 1 contained 75% NB, 0.14% estimated available P (aP), and 0.50% Ca. Diet 2 contained 75% LPB, 0.22% aP, and 0.50% Ca. No inorganic P (iP) was added to Diets 1 and 2 in order to measure the animal response to the different concentrations of aP in these cultivars. Diet 3 was NB Diet 1 supplemented with iP to equal the concentration of aP in LPB Diet 2. Practical barley-soybean meal (SBM)-type diets, NB Diet 4 and LPB Diet 5, were formulated to meet all minimum nutrient requirements, and contained 0.30% aP and 0.65% Ca. For the semipurified diets, pigs fed LPB Diet 2 had higher (P or = 0.3) in growth performance, fresh bone weight, fat-free dry bone weight, bone ash, bone breaking strength, or N utilization. This indicates that LPB and NB were equal in nutritional value after supplementation of NB with iP to equal the estimated aP in LPB. For the practical barley-SBM diets, there were no differences (P > or = 0.4) between pigs fed NB Diet 4 or LPB Diet 5 for growth performance, fresh bone weight, bone breaking strength, the percentages of P and Ca utilization, or N, DE, and ME utilization. The use of LPB in pig diets reduced P excretion in swine waste by 55% and 16% in our semipurified and practical diets, respectively, compared with NB. Using our in vitro procedure designed to mimic the digestive system of the pig, the availability of P for pigs was estimated at 52% for LPB and 32% for NB.

  13. Lowering of phytic acid content by enhancement of phytase and acid phosphatase activities during sunflower germination

    Directory of Open Access Journals (Sweden)

    Juliana da Silva Agostini

    2010-08-01

    Full Text Available The objective of this work was to investigate the germination of hybrid sunflowers BRS191 and C11 as a means of lowering phytic acid (PA content by enhancing the activity of endogenous phytase and acid phosphatase. The concentration of PA in hybrid sunflower achenes varied from 2.16 to 2.83g/100g of sample (p O objetivo deste trabalho foi investigar a germinação de girassóis híbridos BRS 191 e C11 com finalidade de reduzir o teor de AF e aumentar as atividades de phytases e fosfatases endógenas. A concentração do AF nos aquênios de girassóis híbridos variou de 2,16 a 2,83 g /100g de amostra (p< 0,005. As atividades de fitases e fosfatases de girassóis BRS191 e C11 foram elevadas no 4º e 5º dia de germinação, respectivamente, com liberação do fósforo necessário para o desenvolvimento da semente. Estes resultados indicam que o AF do girassol hibrido reduz e a atividade de phytase aumenta em períodos distintos da germinação, possibilitando assim a aplicação desta enzima no controle do teor de AF em cereais, melhorando o seu valor nutricional.

  14. Phytic acid decreases deoxynivalenol and fumonisin B1-induced changes on swine jejunal explants

    Directory of Open Access Journals (Sweden)

    Elisângela Olegário da Silva

    2014-01-01

    Full Text Available The purpose of the present study was to investigate the effects of phytic acid (IP6 on morphological and immunohistochemical parameters on intestinal explants exposed to deoxynivalenol (DON and fumonisin B1 (FB1. The jejunal explants were exposed for 4 h to different treatments: control, DON (10 μM, DON plus 2.5 mM or 5 mM IP6, FB1 (70 μM, and FB1 plus 2.5 mM or 5 mM IP6. Both mycotoxins induced significant intestinal lesions and decreased villi height. The presence of 2.5 mM and 5 mM IP6 significantly inhibited the morphological changes caused by the mycotoxins. DON induced a significant increase in caspase-3 (83% and cyclooxygenase-2 (71.3% expression compared with the control. The presence of 5 mM IP6 induced a significant decrease in caspase-3 (43.7% and Cox-2 (48% expression compared with the DON group. FB1 induced a significant increase in caspase-3 expression (47% compared to the control, whereas IP6 induced no significant change in this expression. A significant decrease in cell proliferation was observed when explants were exposed to 5 mM of IP6 in comparison with the DON and FB1 groups. The present data provide evidence that phytic acid modulates the toxic effects induced by DON and FB1 on intestinal tissue.

  15. Phytic acid decreases deoxynivalenol and fumonisin B1-induced changes on swine jejunal explants.

    Science.gov (United States)

    da Silva, Elisângela Olegário; Gerez, Juliana Rubira; do Carmo Drape, Thalisie; Bracarense, Ana Paula F R L

    2014-01-01

    The purpose of the present study was to investigate the effects of phytic acid (IP6) on morphological and immunohistochemical parameters on intestinal explants exposed to deoxynivalenol (DON) and fumonisin B1 (FB1). The jejunal explants were exposed for 4 h to different treatments: control, DON (10 μM), DON plus 2.5 mM or 5 mM IP6, FB1 (70 μM), and FB1 plus 2.5 mM or 5 mM IP6. Both mycotoxins induced significant intestinal lesions and decreased villi height. The presence of 2.5 mM and 5 mM IP6 significantly inhibited the morphological changes caused by the mycotoxins. DON induced a significant increase in caspase-3 (83%) and cyclooxygenase-2 (71.3%) expression compared with the control. The presence of 5 mM IP6 induced a significant decrease in caspase-3 (43.7%) and Cox-2 (48%) expression compared with the DON group. FB1 induced a significant increase in caspase-3 expression (47%) compared to the control, whereas IP6 induced no significant change in this expression. A significant decrease in cell proliferation was observed when explants were exposed to 5 mM of IP6 in comparison with the DON and FB1 groups. The present data provide evidence that phytic acid modulates the toxic effects induced by DON and FB1 on intestinal tissue.

  16. A decrease in phytic acid content substantially affects the distribution of mineral elements within rice seeds.

    Science.gov (United States)

    Sakai, Hiroaki; Iwai, Toru; Matsubara, Chie; Usui, Yuto; Okamura, Masaki; Yatou, Osamu; Terada, Yasuko; Aoki, Naohiro; Nishida, Sho; Yoshida, Kaoru T

    2015-09-01

    Phytic acid (myo-inositol hexakisphosphate; InsP6) is the storage compound of phosphorus and many mineral elements in seeds. To determine the role of InsP6 in the accumulation and distribution of mineral elements in seeds, we performed fine mappings of mineral elements through synchrotron-based X-ray microfluorescence analysis using developing seeds from two independent low phytic acid (lpa) mutants of rice (Oryza sativa L.). The reduced InsP6 in lpa seeds did not affect the translocation of mineral elements from vegetative organs into seeds, because the total amounts of phosphorus and the other mineral elements in lpa seeds were identical to those in the wild type (WT). However, the reduced InsP6 caused large changes in mineral localization within lpa seeds. Phosphorus and potassium in the aleurone layer of lpa greatly decreased and diffused into the endosperm. Zinc and copper, which were broadly distributed from the aleurone layer to the inner endosperm in the WT, were localized in the narrower space around the aleurone layer in lpa mutants. We also confirmed that similar distribution changes occurred in transgenic rice with the lpa phenotype. Using these results, we discussed the role of InsP6 in the dynamic accumulation and distribution patterns of mineral elements during seed development.

  17. Assessment on proximate composition, dietary fiber, phytic acid and protein hydrolysis of germinated Ecuatorian brown rice.

    Science.gov (United States)

    Cáceres, Patricio J; Martínez-Villaluenga, Cristina; Amigo, Lourdes; Frias, Juana

    2014-09-01

    Germinated brown rice (GBR) is considered healthier than brown rice (BR) but its nutritive value has been hardly studied. Since nutritive quality of GBR depends on genetic diversity and germination conditions, six Ecuadorian BR varieties were germinated at 28 and 34 ºC for 48 and 96 h in darkness and proximate composition, dietary fiber fractions, phytic acid content as well as degree of protein hydrolysis and peptide content were studied. Protein, lipids, ash and available carbohydrate ranged 7.3-10.4%, 2.0-4.0%, 0.8-1.5% and 71.6 to 84.0%, respectively, in GBR seedlings. Total dietary fiber increased during germination (6.1-13.6%), with a large proportion of insoluble fraction, while phytic acid was reduced noticeably. In general, protein hydrolysis occurred during germination was more accused at 28 ºC for 48 h. These results suggest that GBR can be consumed directly as nutritive staple food for a large population worldwide contributing to their nutritional requirements.

  18. Improving the Bioavailability of Seed Phosphorous in Low Phytic Acid Soybean Mutants

    Directory of Open Access Journals (Sweden)

    Ashok Badigannavar and J. G. Manjaya

    2012-03-01

    Full Text Available Phytic acid, the heat stable anti-nutritional factor forms 75% of the total Phosphorous (P in soybean seeds. It acts as strong chelatingagent binding to metal ions reducing the bioavailability of Fe, Zn, Mg and Ca in human and non-ruminant livestock. In the presentstudy, 106 soybean germplasm lines were screened to estimate the seed phytate. It ranged from 0.16 to 4.741mg per g soy flour. Highyielding, low phytate cultivar were selected and subjected to 250 Gy gamma ray irradiation. In M3 generation, mutants having phyticacid content ranged from 0.075 to 2.58 mg/g of soy flour were identified. These mutants have shown as much as 50% or morereduction in seed phytate compared to control. Although low phytic acid line had much higher inorganic ‘P’ concentrations than seedof the normal lines, the balance between protein and oil content was not altered. Since, corn-soy and soymeal are commonly fed tolivestock; reducing phytate content would contribute to increased bioavailability of ‘P’ in these livestock feeds.

  19. Phytic acid and phytase: implications for protein utilization by poultry.

    Science.gov (United States)

    Cowieson, A J; Acamovic, T; Bedford, M R

    2006-05-01

    The effect of the ingestion of myo-inositol hexaphosphate (IP6) and phytase (EC 3.1.3.26) on the digestibility of casein was investigated using growing broiler chickens. A total of 64 female Ross broilers were used in a precision feeding study. One group of 8 birds was fed a solution of glucose to estimate endogenous losses. Seven groups, each of 8 birds, were fed either casein, casein + 1,000 units of phytase activity (FTU), casein + 2,000 FTU, casein + 0.5 g of IP6, casein + 0.5 g of IP6 + 1,000 FTU, casein + 1 g of IP6, or casein + 1 g of IP6 + 1,000 FTU. The excretion of DM, amino acids, nitrogen, minerals, and phytate-phosphorus was determined over a 48-h period and nutrient digestibility coefficients were calculated. Casein was found to be highly digestible, with true coefficients of DM, N, and amino acid digestibility of between 0.85 and 1.0. However, the ingestion of IP6 reduced (P IP6 with phytase improved (P IP6 with no supplemental phytase. The excretion of endogenous minerals was increased (P IP6 and reduced (P IP6 with phytase. In the absence of exogenous phytase, the recovery of phytate-P in excreta was approximately 80%. However, the recovery of phytate-P was significantly reduced by the addition of exogenous phytase to the IP6/casein mixture. It can be concluded that the ingestion of IP6 reduces the digestibility coefficients of amino acids and the metabolizability of nitrogen of casein. This is likely to be mediated partially through increased endogenous losses. However, the addition of phytase can partially ameliorate the detrimental effects of IP6 on protein utilization.

  20. Fast removal of heavy metal ions and phytic acids from water using new modified chelating fiber

    Institute of Scientific and Technical Information of China (English)

    Li Xu; Jin Nan Wang; Ying Meng; Ai Min Li

    2012-01-01

    The graft copolymerization of acrylic acid (AA) onto polyethylene glycol terephthalate (PET) fiber initiated by benzoy peroxide (BPO) was carried out in heterogeneous media.Moreover,modification of the grafted PET fiber (PET-AA) was done by changing the carboxyl group into acylamino group through the reaction with dimethylamine.The modified chelating fiber (NDWJN 1) was characterized using elementary analysis,SEM and FT-IR spectroscopy.Adsorption kinetic curves indicated that NDWJN1 could fast remove heavy metal ions and phytic acids from water effectively.Furthermore,batch kinetic studies indicated that heavy metal ions adsorbed to NDWJN1 could be fitted well by both pseudo-first-order and pseudo-second-order adsorption equations,but the intra-particle diffusion plaved a dominant role in the adsorption of phvtic acids.

  1. Phytic acid/graphene oxide nanocomposites modified electrode for electrochemical sensing of dopamine.

    Science.gov (United States)

    Wang, Donglei; Xu, Fei; Hu, Jiajie; Lin, Meng

    2017-02-01

    An electrochemical sensor for determining dopamine was developed by modifying phytic acid/graphene oxide (PA/GO) nanocomposites onto a glassy carbon electrode (GCE). PA functionalized GO was prepared by an ultra-sonication method. Subsequently, the PA/GO nanocomposites were drop-casted on a glassy carbon substrate. The structural feature of the PA/GO modified GCE was confirmed by attenuated total reflection infrared (ATR-IR) spectroscopy. The proposed electrochemical sensor was applied to detect various concentrations of DA by differential pulse voltammetry (DPV). The PA/GO/GCE was considered to be highly sensitive to DA in the range of 0.05-10μM. In addition, the PA/GO/GCE demonstrated high electrochemical selectivity toward DA in the presence of ascorbic acid (AA) and uric acid (UA). The prepared electrochemical DA sensor was applied for detection of DA in dopamine hydrochloride injection and spiked samples of human urine with satisfactory results.

  2. Fourier Transform Near-Infrared Spectroscopy for rapid and simple determination of phytic acid content in green gram seeds (Vigna radiata).

    Science.gov (United States)

    Pande, Ranjana; Mishra, H N

    2015-04-01

    The feasibility of measuring phytic acid content in green gram (Vigna radiata) seeds was investigated by Fourier Transform Near-Infrared (FT-NIR) spectroscopic technique. Pure phytic acid standards of varying concentrations were scanned using FTNIR spectroscopy. The spectra were measured in diffused reflectance mode by keeping 100-1500 mg/100g standard of pure phytic acid in small sample cuvette. A calibration model was developed using pure phytic acid standards of varying concentrations in the near-infrared region (4000-12,000 cm(-1)). FT-NIR spectroscopy with chemometrics, using the first derivative plus vector normalisation method could predict the phytic acid content in green gram seeds samples. The developed model was validated using cross-validation technique. Maximum coefficient of determination (R(2)) value of 0.97 was obtained for the calibration model developed. The developed model was applied to predict phytic acid content in green gram seeds samples within 1-2 min. The developed procedure was further validated by recovery studies by comparing with UV spectroscopic method of phytic acid determination.

  3. Effects of soaking, germination and fermentation on phytic acid, total and in vitro soluble zinc in brown rice

    NARCIS (Netherlands)

    Liang, J.; Han, B.Z.; Nout, M.J.R.; Hamer, R.J.

    2008-01-01

    Rice is an important staple food in Asian countries. In rural areas it is also a major source of micronutrients. Unfortunately, the bioavailability of minerals, e.g. zinc from rice, is low because it is present as an insoluble complex with food components such as phytic acid. We investigated the eff

  4. Kinetic and Equilibrium Constants of Phytic Acid, Ferric and Ferrous Phytate Derived From Nuclear Magnetic Resonance Spectroscopy

    Science.gov (United States)

    Inositol phosphates are metabolically derived organic phosphates that increasingly appear to be an important sink and source of phosphate in the environment. Inositol hexakis dihydrogen phosphate or phytic acid is the most common inositol phosphate in the environment. Iron is abundant in many terr...

  5. Desalted duck egg white peptides promote calcium uptake by counteracting the adverse effects of phytic acid.

    Science.gov (United States)

    Hou, Tao; Liu, Weiwei; Shi, Wen; Ma, Zhili; He, Hui

    2017-03-15

    The structure of the desalted duck egg white peptides-calcium chelate was characterized by fluorescence spectroscopy, fourier transform infrared spectroscopy, and dynamic light scattering. Characterization results showed structural folding and aggregation of amino acids or oligopeptides during the chelation process. Desalted duck egg white peptides enhanced the calcium uptake in the presence of oxalate, phosphate and zinc ions in Caco-2 monolayers. Animal model indicated that desalted duck egg white peptides effectively enhanced the mineral absorption and counteracted the deleterious effects of phytic acid. These findings suggested that desalted duck egg white peptides might promote calcium uptake in three pathways: 1) desalted duck egg white peptides bind with calcium to form soluble chelate and avoid precipitate; 2) the chelate is absorbed as small peptides by enterocyte; and 3) desalted duck egg white peptides regulate the proliferation and differentiation of enterocytes through the interaction with transient receptor potential vanilloid 6 calcium channel.

  6. Metabolomic characterization of a low phytic acid and high anti-oxidative cultivar of turmeric.

    Science.gov (United States)

    Tanaka, Ken; Arita, Masanori; Li, Donghan; Ono, Naoaki; Tezuka, Yasuhiro; Kanaya, Shigehiko

    2015-02-01

    Turmeric, the rhizome of Curcuma longa, has a long history of use as a spice and also as a traditional medicine in many Asian countries. To reveal unique morphological features of a newly registered Curcuma cultivar, C. longa cv. Okinawa Ougon (Ougon), non-targeted LC-MS and GC-MS analyses were conducted. The analysis revealed its distinctive chemical properties: lower amount of phytic acid and inorganic metals such as Fe, Mn, and Al, as well as higher concentrations of reduced derivatives of curcuminoids, such as dihydrobisdemethoxycurcumin, tetrahydrobisdemethoxycurcumin, dihydrodemethoxycurcumin, and tetrahydrodemethoxycurcumin. In addition, germacrane-type sesquiterpenes were almost absent although α-humulene and β-caryophyllene, generated by the same biosynthetic route, were present. Presumably the alternation of the metal ion content, serving as a cofactor of sesquiterpene synthase, modulates the resulting variation of the sesquiterpenes. In summary, the cultivar Ougon is considered a promising candidate for functional food additives.

  7. Microstructure and corrosion resistance of phytic acid conversion coatings for magnesium alloy

    Energy Technology Data Exchange (ETDEWEB)

    Cui Xiufang [School of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin 150001 (China); State Key Laboratory for Corrosion and Protection, Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016 (China); Li Qingfen [School of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin 150001 (China); Li Ying; Wang Fuhui [State Key Laboratory for Corrosion and Protection, Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016 (China); Jin Guo [School of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin 150001 (China)], E-mail: jg97721@yahoo.com.cn; Ding Minghui [School of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin 150001 (China)

    2008-12-30

    In this paper, a new innoxious and pollution-free chemical protective coating for magnesium alloys, phytic acid conversion coating, was prepared. The conversion coatings are found to have high cover ratio and no cracks are found by atomic force microscopes (AFM) and scanning electron microscopy (SEM). The main elements of the conversion coatings are Mg, Al, O, P and C by X-ray photoelectron spectroscopy (XPS) and Auger electron spectroscopy (AES). The chemical state of the elements in the coatings was also investigated by Fourier transform infrared spectroscopy (FTIR). AES depth profile analysis suggests that the thickness of the conversion coating is about 340 nm. The corrosion resistance of the coatings was evaluated by polarization curves. The results indicate that the corrosion resistance for the conversion coated AZ91D magnesium alloys in 3.5% NaCl solution increases markedly. The mechanisms of corrosion resistance and coatings formation are also discussed.

  8. Microstructure and corrosion resistance of phytic acid conversion coatings for magnesium alloy

    Science.gov (United States)

    Cui, Xiufang; Li, Qingfen; Li, Ying; Wang, Fuhui; Jin, Guo; Ding, Minghui

    2008-12-01

    In this paper, a new innoxious and pollution-free chemical protective coating for magnesium alloys, phytic acid conversion coating, was prepared. The conversion coatings are found to have high cover ratio and no cracks are found by atomic force microscopes (AFM) and scanning electron microscopy (SEM). The main elements of the conversion coatings are Mg, Al, O, P and C by X-ray photoelectron spectroscopy (XPS) and Auger electron spectroscopy (AES). The chemical state of the elements in the coatings was also investigated by Fourier transform infrared spectroscopy (FTIR). AES depth profile analysis suggests that the thickness of the conversion coating is about 340 nm. The corrosion resistance of the coatings was evaluated by polarization curves. The results indicate that the corrosion resistance for the conversion coated AZ91D magnesium alloys in 3.5% NaCl solution increases markedly. The mechanisms of corrosion resistance and coatings formation are also discussed.

  9. Phytic acid adsorption on the copper surface: Observation of electrochemistry and Raman spectroscopy

    Science.gov (United States)

    Shen, Shu; Guo, Xiao-yu; Song, Ping; Pan, Ying-Cheng; Wang, Hao-qiong; Wen, Ying; Yang, Hai-Feng

    2013-07-01

    The adsorption of phytic acid (PA) on copper was investigated using electrochemical impedance spectroscopy (EIS), electrochemical polarization measurement and surface-enhanced Raman scattering (SERS) spectroscopy. Electrochemical results indicated that inhibition efficiency of PA film for copper from corrosion in 3 wt% NaCl solution was beyond 80% at an optimum self-assembly concentration of 0.1 mM for 6 h. Electrochemical polarization indicated that PA functioned as a cathodic inhibitor. In addition, Raman studies showed that PA adsorbed on the copper surface formed via P-O groups. Finally, the value of ΔGads (-39.96 kJ mol-1) was close to -40 kJ mol-1, suggesting that the adsorption of PA on the copper surface was the chemical adsorption.

  10. Generation and characterization of low phytic acid germplasm in rice (Oryza sativa L.).

    Science.gov (United States)

    Liu, Qing-Long; Xu, Xiu-Hong; Ren, Xue-Liang; Fu, Hao-Wei; Wu, Dian-Xing; Shu, Qing-Yao

    2007-03-01

    Phytic acid (PA, myo-inositol 1,2,3,4,5,6-hexakisphosphate), or its salt form, phytate, is commonly regarded as the major anti-nutritional component in cereal and legume grains. Breeding of low phytic acid (lpa) crops has recently been considered as a potential way to increase nutritional quality of crop products. In this study, eight independent lpa rice mutant lines from both indica and japonica subspecies were developed through physical and chemical mutagenesis. Among them, five are non-lethal while the other three are homozygous lethal. None of the lethal lines could produce homozygous lpa plants through seed germination and growth under field conditions, but two of them could be rescued through in vitro culture of mature embryos. The non-lethal lpa mutants had lower PA content ranging from 34 to 64% that of their corresponding parent and four of them had an unchanged total P level. All the lpa mutations were inherited in a single recessive gene model and at least four lpa mutations were identified mutually non-allelic, while the other two remain to be verified. One mutation was mapped on chromosome 2 between microsatellite locus RM3542 and RM482, falling in the same region as the previously mapped lpa1-1 locus did; another lpa mutation was mapped on chromosome 3, tightly linked to RM3199 with a genetic distance of 1.198 cM. The latter mutation was very likely to have happened to the LOC_Os03g52760, a homolog of the maize myo-inositol kinase (EC 2.7.1.64) gene. The present work greatly expands the number of loci that could influence the biosynthesis of PA in rice, making rice an excellent model system for research in this area.

  11. Enhanced absorption of anthocyanins after oral administration of phytic acid in rats and humans.

    Science.gov (United States)

    Matsumoto, Hitoshi; Ito, Kyoko; Yonekura, Kumiko; Tsuda, Takanori; Ichiyanagi, Takashi; Hirayama, Masao; Konishi, Tetsuya

    2007-03-21

    Many studies on the bioavailability of polyphenols have been reported. However, the relative urinary excretions of AC are also low, ranging from 0.004% to 0.1%. By contrast, other polyphenols show higher urinary excretion levels. Here, we studied the enhancing effects of phytic acid (IP6) on absorption of blackcurrant anthocyanins (BCAs) in rats and humans. In rats after oral administration of BCAs (as 241 mg of AC/kg body weight) in IP6 (0%, 0.25%, 0.5%, 1%, 2.5%) solution, the ACs recovery in urine was increased dependent on IP6 dose. These results suggest that the IP6 enhances gastrointestinal absorption of ACs. At the further analysis of IP6 enhancement effect in rat, whereas BCAs were normally passed through the stomach and duodenum within 2 h, in IP6 group, after 2-6 h post-administration, stomach and jejunum content's weights were specifically heavy, and large amounts of ACs were also detected in stomach, duodenum, and jejunum. These results suggested that the mixture of BCAs and IP6 reduced the gastrointestinal motility. Prolongation of ACs residue in gastrointestinal tract then caused the enhancing effects of IP6 on absorption of AC. In the human study, each subject was orally administrated a BCA beverage containing BCA concentrate (AC 4 mg/kg body weight), 1% of IP6, and 1% of sodium citrate as a pH stabilizer. Both the plasma level and the urinary excretion of AC were increased as compared to BCA administration without IP6. AC intake with IP6 may increase the bioavailability of AC to the comparative level as other polyphenols. Yet, phytic acid, being a strong chelator of important minerals, contributes to mineral deficiencies. An interference with iron uptake has been reported. Safety tests are therefore necessary before high dose IP6 can be used in foods.

  12. In vitro availability of zinc from infant foods with increasing phytic acid contents.

    Science.gov (United States)

    Bosscher, D; Lu, Z; Janssens, G; Van Caillie-Bertrand, M; Robberecht, H; De Rycke, H; De Wilde, R; Deelstra, H

    2001-08-01

    An in vitro method was used to determine the availability of Zn from infant foods containing increasing amounts of phytate, and to quantify the effect of the phytate:Zn molar ratio on the availability. During the in vitro assay, digestive conditions of infants, younger and older than 4 months of age, were carefully simulated since the solubility of phytate-Zn complexes during digestion is pH dependent. Availability was measured with a continuous flow dialysis in vitro procedure with previous intralumen digestive stage. Zn concentrations were determined with flame atomic absorption spectrometry. Phytic acid content was measured with HPLC. Adding phytate to infant formula lowered Zn availability to 2.84 (sd 0.17) % when the phytate:Zn molar ratio increased to 2.2 as compared with cows' milk-based formula (6.65 (sd 0.55) %). Availability from vegetables (23.83 (sd 2.17) %) significantly decreased at a ratio > 7.9 (15.12 (sd 1.63) %). Zn availability from soyabean-based formula (2.26 (sd 0.36) %) was lower compared with cows' milk-based formula (6.65 (sd 0.55) %). Availability between soyabean- and cows' milk-based formula was similar when a phytate:Zn ratio of 2.2 (2.84 (sd 0.17) %) was obtained in the cows' milk formula. The negative effect of phytic acid on Zn availability was dependent on the type of the food and the phytate content, and should be considered when using soyabean-based formulas during early infancy.

  13. Phytic acid degrading lactic acid bacteria in tef-injera fermentation.

    Science.gov (United States)

    Fischer, Maren M; Egli, Ines M; Aeberli, Isabelle; Hurrell, Richard F; Meile, Leo

    2014-11-03

    Ethiopian injera, a soft pancake, baked from fermented batter, is preferentially prepared from tef (Eragrostis tef) flour. The phytic acid (PA) content of tef is high and is only partly degraded during the fermentation step. PA chelates with iron and zinc in the human digestive tract and strongly inhibits their absorption. With the aim to formulate a starter culture that would substantially degrade PA during injera preparation, we assessed the potential of microorganisms isolated from Ethiopian household-tef fermentations to degrade PA. Lactic acid bacteria (LAB) were found to be among the dominating microorganisms. Seventy-six isolates from thirteen different tef fermentations were analyzed for phytase activity and thirteen different isolates of seven different species were detected to be positive in a phytase screening assay. In 20-mL model tef fermentations, out of these thirteen isolates, the use of Lactobacillus (L.) buchneri strain MF58 and Pediococcus pentosaceus strain MF35 resulted in lowest PA contents in the fermented tef of 41% and 42%, respectively of its initial content. In comparison 59% of PA remained when spontaneously fermented. Full scale tef fermentation (0.6L) and injera production using L. buchneri MF58 as culture additive decreased PA in cooked injera from 1.05 to 0.34±0.02 g/100 g, representing a degradation of 68% compared to 42% in injera from non-inoculated traditional fermentation. The visual appearance of the pancakes was similar. The final molar ratios of PA to iron of 4 and to zinc of 12 achieved with L. buchneri MF58 were decreased by ca. 50% compared to the traditional fermentation. In conclusion, selected LAB strains in tef fermentations can degrade PA, with L. buchneri MF58 displaying the highest PA degrading potential. The 68% PA degradation achieved by the application of L. buchneri MF58 would be expected to improve human zinc absorption from tef-injera, but further PA degradation is probably necessary if iron absorption has to

  14. Identification and characterization of the soybean IPK1 ortholog of a low phytic acid mutant reveals an exon-excluding splice-site mutation.

    Science.gov (United States)

    Yuan, Feng-Jie; Zhu, Dan-Hua; Tan, Yuan-Yuan; Dong, De-Kun; Fu, Xu-Jun; Zhu, Shen-Long; Li, Bai-Quan; Shu, Qing-Yao

    2012-11-01

    Phytic acid (myo-inositol 1, 2, 3, 4, 5, 6 hexakisphosphate) is an important constituent of soybean meal. Since phytic acid and its mineral salts (phytates) are almost indigestible for monogastrics, their abundance in grain food/feed causes nutritional and environmental problems; interest in breeding low phytic acid has therefore increased considerably. Based on gene mapping and the characteristics of inositol polyphosphates profile in the seeds of a soybean mutant line Gm-lpa-ZC-2, the soybean ortholog of inositol 1,3,4,5,6 pentakisphosphate (InsP(5)) 2-kinase (IPK1), which transforms InsP(5) into phytic acid, was first hypothesized as the candidate gene responsible for the low phytic acid alteration in Gm-lpa-ZC-2. One IPK1 ortholog (Glyma14g07880, GmIPK1) was then identified in the mapped region on chromosome 14. Sequencing revealed a G → A point mutation in the genomic DNA sequence and the exclusion of the entire fifth exon in the cDNA sequence of GmIPK1 in Gm-lpa-ZC-2 compared with its wild-type progenitor Zhechun No. 3. The excluded exon encodes 37 amino acids that spread across two conserved IPK1 motifs. Furthermore, complete co-segregation of low phytic acid phenotype with the G → A mutation was observed in the F(2) population of ZC-lpa x Zhexiandou No. 4 (a wild-type cultivar). Put together, the G → A point mutation affected the pre-mRNA splicing and resulted in the exclusion of the fifth exon of GmIPK1 which is expected to disrupt the GmIPK1 functionality, leading to low phytic acid level in Gm-lpa-ZC-2. Gm-lpa-ZC-2, would be a good germplasm source in low phytic acid soybean breeding.

  15. Anion-exchange high-performance liquid chromatography with post-column detection for the analysis of phytic acid and other inositol phosphates

    Science.gov (United States)

    Rounds, M. A.; Nielsen, S. S.; Mitchell, C. A. (Principal Investigator)

    1993-01-01

    The use of gradient anion-exchange HPLC, with a simple post-column detection system, is described for the separation of myo-inositol phosphates, including "phytic acid" (myo-inositol hexaphosphate). Hexa-, penta-, tetra-, tri- and diphosphate members of this homologous series are clearly resolved within 30 min. This method should facilitate analysis and quantitation of "phytic acid" and other inositol phosphates in plant, food, and soil samples.

  16. Genetic analysis of two OsLpa1-like genes in Arabidopsis reveals that only one is required for wild-type seed phytic acid levels

    Science.gov (United States)

    Phytic acid (inositol-1,2,3,4,5,6-hexakisphosphate or InsP6) is the primary storage form of phosphorus in plant seeds. The rice OsLpa1 encodes a novel protein required for wild-type levels of seed InsP6 and was identified from a low phytic acid (lpa) mutant exhibiting a 45-50% reduction in seed InsP...

  17. Preservation Effect of Phytic Acid on Tomato%植酸对番茄保鲜效果的影响

    Institute of Scientific and Technical Information of China (English)

    任邦来; 赵波

    2012-01-01

    以变色期番茄为材料,分别采用浓度为0.05%、0.1O%、0.15%的植酸溶液浸泡30s,在室温下贮藏33d,研究不同浓度植酸溶液涂膜处理对番茄果实的保鲜效果。结果表明,植酸溶液处理能推迟番茄果实呼吸跃变时呼吸高峰的出现时间,降低呼吸强度峰值;对保持果实硬度、可溶性固形物、维生素C、可滴定酸有较好作用;能减少水分蒸发,降低果实失重率;其中浓度为0.1O%的植酸溶液对番茄果实进行涂膜处理保鲜效果最好。%The deteriorated tomotoes were soaked for 30s with phytic acid of 0.05%, 0.10%, and 0.15% and stored for up to 33d at regular temperature. The effects of preservation Of different concentrations of phytic acid in the coating treatment on tomatoes were studied. The results showed that phytic acid could inhabit the peak time of respiratory rate of the climacteric in ripening tomato fruit, reduce the respiration rate and could keep the hardness, soluble solids, titratable acid and vitamin C of tomoto. Meanwhile,it could decrease the water evaporation and weightlessness rate of fruit. The best concentration of phytic acid that coated tomotoes fruit in preservation was 0.10%.

  18. Iron absorption from experimental infant formulas based on pea (Pisum sativum)-protein isolate: the effect of phytic acid and ascorbic acid.

    Science.gov (United States)

    Davidsson, L; Dimitriou, T; Walczyk, T; Hurrell, R F

    2001-01-01

    Infant formula based on pea (Pisum sativum)-protein isolate has been suggested as an alternative to soybean formula in countries where soybean is not a native crop, or when soybean protein cannot be used due to allergic reactions or intolerances. In the present study, Fe absorption from experimental infant formulas based on pea-protein isolate was measured in healthy non-anaemic young women. The influence of phytic acid and ascorbic acid on Fe absorption was evaluated, using a stable-isotope technique based on incorporation of Fe stable-isotope labels into erythrocytes 14 d after administration. Geometric mean Fe absorption increased from 20.7 (+1 SD 41.6, -1 SD 10.3) % to 33.1 (+1 SD 58.6, -1 SD 18.7) %; (P phytic acid. Doubling the molar ratio Fe:ascorbic acid from 1:2.1 to 1:4.2 in the infant formula with native phytic acid content also increased Fe absorption significantly (P phytic acid and ascorbic acid respectively on Fe absorption, but also indicate relatively high fractional Fe absorption from the pea-protein-based formulas. After adjusting for differences in Fe status, our data indicate that Fe absorption from dephytinised pea protein might be less inhibitory than dephytinised soybean protein as measured in a previous study (Hurrell et al. 1998).

  19. Bioavailability of magnesium from inorganic and organic compounds is similar in rats fed a high phytic acid diet.

    Science.gov (United States)

    Bertinato, Jesse; Plouffe, Louise J; Lavergne, Christopher; Ly, Catherine

    2014-01-01

    A large section of the North American population is not meeting recommended intakes for magnesium (Mg). Supplementation and consumption of Mg-fortified foods are ways to increase intake. Currently, information on Mg bioavailability from different compounds and their efficacy in improving Mg status is scant. This study compared the relative ability of inorganic and organic Mg compounds to preserve the Mg status of rats when fed at amounts insufficient to retain optimal Mg status. Male Sprague-Dawley rats (n=12/diet group) were fed one of eight test diets supplemented with phytic acid (5 g/kg diet) and low levels of Mg (155 mg elemental Mg/kg diet) from Mg oxide, Mg sulphate, Mg chloride, Mg citrate, Mg gluconate, Mg orotate, Mg malate or ethylenediaminetetraacetic acid disodium Mg salt for five weeks. Rats were also fed three control diets that did not contain added phytic acid but were supplemented with 500 (NMgO, normal), 155 (LMgO, low) or 80 (DMgO, deficient) mg of Mg per kg diet as Mg oxide. Mg concentrations in femur, serum and urine showed a graded decrease in rats fed the control diets with lower Mg. Mg concentrations did not differ (P≥0.05) between rats fed the different test diets. Addition of phytic acid to the diet did not affect the Mg status of the rats. The results indicate that any differences in the Mg bioavailability of the compounds were small and physiologically irrelevant.

  20. Correlations between the contents of phytic acid and inorganic phosphorous and downy mildew resistance of corn inbred lines

    OpenAIRE

    Pantipa Na Chiangmai*; Phrutiya Nilprapruck; Warapon Bunkoed; Phakatip Yodmingkhwan; Chokechai Aekatasanawan; Mana Kanjanamaneesathian

    2015-01-01

    Seeds of corn inbred lines collected at the National Corn and Sorghum Research Center (NCSRC), Kasetsart University, were analyzed to determine the contents of phytic acid (PA) and inorganic phosphorous (InP). These 28 and 29 inbred lines were cultivated at the NCSRC (in the 2008 late rainy season and 2009 early rainy season) to evaluate their resistance to corn downy mildew caused by Peronosclerospora sorghi. Results showed that the values of the PA, InP contents and downy mildew...

  1. Effect of particle size reduction, hydrothermal and fermentation treatments on phytic acid content and some physicochemical properties of wheat bran.

    Science.gov (United States)

    Majzoobi, Mahsa; Pashangeh, Safoora; Farahnaky, Asgar; Eskandari, Mohammad Hadi; Jamalian, Jalal

    2014-10-01

    With the aim of reducing phytic acid content of wheat bran, particle size reduction (from 1,200 to 90 μm), hydrothermal (wet steeping in acetate buffer at pH 4.8 at 55 °C for 60 min) and fermentation (using bakery yeast for 8 h at 30 °C) and combination of these treatments with particle size reduction were applied and their effects on some properties of the bran were studied. Phytic acid content decreased from 50.1 to 21.6, 32.8 and 43.9 mg/g after particle size reduction, hydrothermal and fermentation, respectively. Particle size reduction along with these treatments further reduced phytic acid content up to 76.4 % and 57.3 %, respectively. Hydrothermal and fermentation decreased, while particle size reduction alone or in combination increased bran lightness. With reducing particle size, total, soluble and insoluble fiber content decreased from 69.7 to 32.1 %, 12.2 to 7.9 % and 57.4 to 24.3 %, respectively. The highest total (74.4 %) and soluble (21.4 %) and the lowest insoluble fiber (52.1 %) content were determined for the hydrothermaled bran. Particle size reduction decreased swelling power, water solubility and water holding capacity. Swelling power and water holding capacity of the hydrothermaled and fermented brans were lower, while water solubility was higher than the control. The amount of Fe(+2), Zn(+2) and Ca(+2) decreased with reducing particle size. Fermentation had no effect on Fe(+2)and Zn(+2) but slightly reduced Ca(+2). The hydrothermal treatment slightly decreased these elements. Amongst all, hydrothermal treatment along with particle size reduction resulted in the lowest phytic acid and highest fiber content.

  2. Dynamic changes in the distribution of minerals in relation to phytic acid accumulation during rice seed development.

    Science.gov (United States)

    Iwai, Toru; Takahashi, Michiko; Oda, Koshiro; Terada, Yasuko; Yoshida, Kaoru T

    2012-12-01

    Phytic acid (inositol hexakisphosphate [InsP(6)]) is the storage compound of phosphorus in seeds. As phytic acid binds strongly to metallic cations, it also acts as a storage compound of metals. To understand the mechanisms underlying metal accumulation and localization in relation to phytic acid storage, we applied synchrotron-based x-ray microfluorescence imaging analysis to characterize the simultaneous subcellular distribution of some mineral elements (phosphorus, calcium, potassium, iron, zinc, and copper) in immature and mature rice (Oryza sativa) seeds. This fine-imaging method can reveal whether these elements colocalize. We also determined their accumulation patterns and the changes in phosphate and InsP(6) contents during seed development. While the InsP(6) content in the outer parts of seeds rapidly increased during seed development, the phosphate contents of both the outer and inner parts of seeds remained low. Phosphorus, calcium, potassium, and iron were most abundant in the aleurone layer, and they colocalized throughout seed development. Zinc was broadly distributed from the aleurone layer to the inner endosperm. Copper localized outside the aleurone layer and did not colocalize with phosphorus. From these results, we suggest that phosphorus translocated from source organs was immediately converted to InsP(6) and accumulated in aleurone layer cells and that calcium, potassium, and iron accumulated as phytic acid salt (phytate) in the aleurone layer, whereas zinc bound loosely to InsP(6) and accumulated not only in phytate but also in another storage form. Copper accumulated in the endosperm and may exhibit a storage form other than phytate.

  3. THE EFFECT OF PHYTIC ACID, ZINC AND SOYBEAN EXTRACT ON THE GROWTH AND AFLATOXIN B1 PRODUCTION BY Aspergillus flavus

    OpenAIRE

    Sardjono, Sardjono

    2012-01-01

    It has been reported that aflatoxin contamination in soybean was relatively low, but it was not guaranteed that soybean products is free from aflatoxin contamination. Naturally, soybean containing phytic acid and it bound zinc and protein. Zinc (Zn) is an important mineral for aflatoxin biosynthesis. Previous research indicated that some soybean products such as kecap was contaminated by aflatoxin. It might be Aspergillus flavus involved during kecap fermentation and it produced phytase for p...

  4. Reduction in phytic acid content and enhancement of antioxidant properties of nutricereals by processing for developing a fermented baby food

    OpenAIRE

    Rasane, Prasad; Jha, Alok; Kumar, Arvind; Sharma, Nitya

    2014-01-01

    Cereal blends containing pearl millet (Pennisetum glaucum), sorghum (Sorghum bicolor) and oat (Avena sativa) in different ratios were processed (roasted and germinated) and also used as unprocessed flours followed by fermentation with Lactobacillus sp. (Lactobacillus casei and Lactobacillus plantarum). They were screened for total phenolic content (TPC), phytic acid content (PAC) and free radical scavenging activity (FRSA). A formulation with the highest TPC, FRSA and the lowest PAC was selec...

  5. Effect of different debittering processes on mineral and phytic acid content of lupin (Lupinus albus L.) seeds.

    Science.gov (United States)

    Ertaş, Nilgün; Bilgiçli, Nermin

    2014-11-01

    Lupin is a valuable ancient legume which contains high amount of protein, dietary fiber, oil, minerals and different functional components. Bitter lupin seeds cannot be consumed directly since its high toxic alkaloid content. Cooking and soaking are effective processes for removing these toxic substances and antinutrients as phytic acid, trypsin inhibitors and oligosaccharides. In this study, debittering process containing cooking and soaking up to 144 h was applied to lupin seeds. Raw lupin seeds had 3.3 % ash and 41.3 % protein content. Ash and protein content of debittered seeds changed between 2.1 and 2.5 %, 39.5 and 40.9 % respectively. After debittering process, significant (p Phytic acid was removed from raw lupin seeds up to 71.4 % ratio by debittering processes, and soaking in distilled water at 55 °C and long soaking time (144 h) was found the most effective methods on phytic acid loss. While more lighter (L*) seeds were obtained with soaking in distilled water at 25 °C, soaking in 0.5 % NaHCO3 solution gave more yellowish (b*) seed properties compared to other soaking methods. Soaking in 0.5 % NaHCO3 solution at 144 h gave the most liked products in terms of sensorial evaluation.

  6. Phenolics, phytic acid, and phytase in Canadian-grown low-tannin faba bean (Vicia faba L.) genotypes.

    Science.gov (United States)

    Oomah, B Dave; Luc, Geneviéve; Leprelle, Claire; Drover, John C G; Harrison, Judith E; Olson, Mark

    2011-04-27

    Thirteen low-tannin faba bean genotypes grown at two locations in north central Alberta in 2009 were evaluated to investigate the variation in seed characteristics, phenolic and phytate contents, and phytase and antioxidant activities and to elucidate the relationship of these components as a breeding strategy. Seed characteristics including color were predominantly genotype dependent. The faba bean genotypes with total phenolic content ranging from 5.5 to 41.8 mg of catechin equiv/g of sample was linearly related to tannin content and the best predictor of antioxidant activity. Phytic acid content and phytase activity varied significantly among genotypes and between locations, ranging from 5.9 to 15.1 g/kg and from 1606 to 2154 FTU/kg sample, respectively. Multivariate data analysis performed on 19 components analyzed in this study using principal component analysis (PCA) and cluster analysis demonstrate that differences in seed characteristics, phenolic components, phytic acid, and phytase are major factors in segregating faba bean genotypes. The relatively low phytic acid content and high phytase activity of these low-tannin faba bean genotypes are beneficial/essential traits for their use in human and animal nutrition.

  7. Possible Causes of Ileal Injury in Two Models of Microbial Sepsis and Protective Effect of Phytic Acid

    Directory of Open Access Journals (Sweden)

    Rasha Rashad Ahmed

    2010-03-01

    Full Text Available Background: Sepsis related-multiple organ dysfunction is associatedwith ileum injury. We aimed to determine the causes ofileal injury in two models of microbial sepsis resulted from infectionwith Aeromonas hydrophila or its endotoxin. We alsoevaluated the protective effect of phytic acid.Methods: Thin sections of ileum from 60 Swiss male mice incontrol, bacteria-infected or lipopolysaccharides (LPS andbacteria-infected or LPS-infected co-administered with phyticacid were subjected to histopathological and TdT-mediateddUTP nick-end labeling (TUNEL assay for apoptotic cellsdetection while ultra thin sections were stained with uranylacetate and lead citrate for cytological changes examination.Also, ileum images were exposed to the image analysis softwareto determine some related morphometric measures.Results: Necrosis and apoptosis were observed in ileum injuryin both examined sepsis models. The ileum injury was moresevere in LPS model. Phytic acid showed the ability to attenuateileum injury in Aeromonas hydrophila and its endotoxinmodels of sepsis after four weeks administration where itssupplementation significantly minimized the histopathologicaland cytological complications and morphometric alterationsresulted from the injury.Conclusion: The protective effects of phytic acid may becaused by increased mucous secretion, decreased apoptoticindex, attenuating the inflammatory and lymphocytic cellscount or increasing the renewal of the crypt cells and villousepithelial cells proliferation.

  8. Ileal digestibility and endogenous flow of minerals and amino acids: responses to dietary phytic acid in piglets.

    Science.gov (United States)

    Woyengo, Tofuko A; Cowieson, Aaron J; Adeola, Olayiwola; Nyachoti, Charles M

    2009-08-01

    Effects of phytic acid (PA) on ileal mineral and amino acid (AA) digestibilities and ileal endogenous AA flow in piglets were investigated. Seven ileal-cannulated weanling pigs were fed a casein-maize starch-based diet with PA (as sodium phytate) at 0, 5, 10 or 20 g/kg in 4 x 4 Latin square design with three added columns to give seven observations per treatment. The basal diet was formulated to meet National Research Council energy and AA requirements for piglets. The respective digestibility and endogenous lysine loss were determined by indicator and homoarginine methods. The apparent ileal digestibility (AID) of Na, K and P was linearly and quadratically reduced (P 0.05) endogenous AA losses. The results suggest that PA has limited effect on the digestibility and endogenous losses of AA in piglets, but can reduce AID of Mg and Na partly by increasing endogenous losses of these minerals as evidenced by their negative AID values.

  9. Low-phytic acid corn improves nutrient utilization for growing pigs.

    Science.gov (United States)

    Veum, T L; Ledoux, D R; Raboy, V; Ertl, D S

    2001-11-01

    Thirty-five crossbred barrows averaging 14.5 kg initial BW were used in a 5-wk experiment to compare the P availability and nutritional value of a low-phytate hybrid corn (LPC, 0.26% total P, 0.08% phytic acid P) homozygous for the lpa 1-1 allele with a nearly isogenic normal hybrid corn (NC, 0.25% total P, 0.20% phytic acid P). The pigs were fed individually twice daily in metabolism pens. Three semipurified diets were created in which corn was the only source of phytate. Diet 1 contained 72% NC, 0.15% estimated available P (aP) and 0.55% Ca. Diet 2 contained 72% LPC, 0.24% aP, and 0.55% Ca. The only differences between Diets 1 and 2 were the source of corn and the levels of aP. No inorganic P (iP) was added to these diets in order to measure the animal response to the different levels of aP in the corn hybrids. Diet 3 was NC Diet 1 supplemented with iP to equal the level of aP in LPC Diet 2. Diets 4 and 5 were practical corn-soybean meal diets formulated with each corn to meet all minimum nutrient requirements and contained 0.30% aP and 0.65% Ca. For the semipurified diets, pigs fed LPC Diet 2 had higher (P or = 0.2), indicating an equal nutritional value for both corn hybrids after adjusting for phytate level. The only treatment difference, other than P excretion, between the practical corn diets supplemented with soybean meal was a higher (P < 0.05) bone breaking strength for pigs fed LPC Diet 5 compared with NC Diet 4. The use of LPC in pig diets reduced P excretion in swine waste by 50 and 18.4% in the semipurified and practical diets, respectively, compared with NC. Using our in vitro procedure designed to simulate the digestive system of the pig, the availability of P for pigs was estimated at 56% for LPC and 11% for NC.

  10. Effects of low-phytic acid corn, low-phytic acid soybean meal, and phytase on nutrient digestibility and excretion in growing pigs.

    Science.gov (United States)

    Hill, B E; Sutton, A L; Richert, B T

    2009-04-01

    Forty-eight grower pigs were used to evaluate the effects of feeding low phytic acid (LPA) corn, LPA soybean meal, normal corn (NC), normal soybean meal (NSBM), and phytase on nutrient digestibility and excretion. Barrows were blocked by BW (initial BW=45.3+/-1.6 kg) and randomly assigned to 1 of 8 dietary treatments in a 2 x 2 x 2 factorial arrangement (6 pigs/treatment). Pigs were fed twice daily (0700 and 1700 h) at 3 times the ME requirement for maintenance. Phytase was added to the diet at 510 phytase units/kg of feed (where 1 phytase unit is the quantity of enzyme that liberates 1 mumol of inorganic P/min from 0.005 mol/L of sodium phytate at pH 5.5 and 37 degrees C), at the expense of corn starch, and all diets were formulated to provide 0.39% total P, 0.50% Ca, and 1.0% lysine with no supplemental inorganic P. Pigs were adapted to metabolism crates and dietary treatments for 7 d, followed by a 3-d total collection of urine and feces. Total fecal DM excreted, percentage of DM of feces, and percentage of DM digested were not different (P>0.53) among treatments. Fecal P excretion was reduced for pigs fed LPA corn vs. NC (2.85 vs. 3.24+/-0.119 g/d; P=0.024), for pigs fed LPA soybean meal vs. NSBM (2.79 vs. 3.30+/-0.119 g/d; P=0.007), for pigs fed phytase vs. nonphytase diets (2.80 vs. 3.29+/-0.119 g/d; P=0.009), and for pigs fed LPA corn, LPA soybean meal, and phytase vs. NC and NSBM without phytase (2.16 vs. 3.70+/-0.237 g/d; Psoybean meal, and phytase diet vs. the NC and soybean meal diet (60.1 vs. 34.1+/-4.5%; Psoybean meal vs. NSBM (47.2 vs. 41.1+/-2.27%; P=0.075). Corn type and soybean meal type had no effect (P>0.11) on water-soluble P excretion. However, pigs fed diets containing phytase tended to excrete less total water-soluble P than those without phytase inclusion (1.99 vs. 2.27+/-0.099 g/d; Psoybean meal, and phytase was additive, significantly improving P digestibility and dramatically decreasing P excretion to reduce the potential impacts of P

  11. Effects of lactoferrin, phytic acid, and EDTA on oxidation in two food emulsions enriched with long-chain polyunsaturated fatty acids

    DEFF Research Database (Denmark)

    Nielsen, Nina Skall; Petersen, A.; Meyer, Anne S.

    2004-01-01

    to 9 weeks. Mayonnaise samples with 16% fish oil and 64% rapeseed oil (w/w) were supplemented with either lactoferrin (8-32 M), phytic acid (16-124 M), or EDTA (16-64 M) and were stored at 20 C for up to 4 weeks. The effect of the metal chelators was evaluated by determination of peroxide values...

  12. [Blocking effect of phytic acid on cell proliferation in human gastric carcinoma].

    Science.gov (United States)

    Wang, Lu; Yang, Zhiping; Cui, Hongbin

    2008-05-01

    To explore the bcl-2 and the bax protein expression, the effect and possible mechanism of phytic acid (IP6) on cell proliferation in human gastric carcinoma. The inhibiting action of IP6 on human gastric carcinoma was examed by MTT assay. The morphological changes of SGC-7901 cells exposed to IP6 was examined by reverse discrepancy microscope. The apoptosis of SGC-7901 cells treated with IP6 was observed by single cell gel electrophoresis. The bax and bcl-2 protein expressions were detected by Western blotting method. MTT assay indicated that the growth of SGC-7901 cells were inhibited by IP6 in dose and time dependent manners. The morphological observation by reverse discrepancy microscope indicated that the growth of cells exposed to IP6 were not well. The DNA damage rates of SGC-7901 cells treated with IP6 were more higher than those of control groups in dose and time dependent manners. The bcl-2 protein expressions treated with IP6 were reduced, and the bax protein expressions treated with IP6 were more than those of control groups in dose and time dependent manners. The proliferation of gastric carcinoma SGC-7901 cells inhibitited by IP6 could be associated with apoptosis of gene bax and bcl-2.

  13. Influence of phytic acid concentration on coating properties obtained by MAO treatment on magnesium alloys

    Science.gov (United States)

    Zhang, R. F.; Zhang, S. F.; Duo, S. W.

    2009-06-01

    Anodic coatings were prepared by microarc oxidation (MAO) on AZ91HP in a base solution of 10 g/L NaOH with and without the addition of 0-12 g/L phytic acid (C 6H 18O 24P 6). The influences of C 6H 18O 24P 6 and its concentration on the conductivity and breakdown voltage were studied. The morphologies and compositions of anodic coatings were determined by environmental scanning electron microscope (ESEM) and energy dispersive X-ray spectroscopy (EDX). Potentiodynamic polarization test was performed in 3.5 wt.% NaCl solution to evaluate the corrosion resistance of anodic coatings. The results showed that with the increase of C 6H 18O 24P 6 concentration, the solution conductivity decreased while the values of breakdown voltage increased. EDX analysis showed that the coatings formed in solutions with C 6H 18O 24P 6 addition contained Mg, Al, O, C, P and a trance of Na. The addition of C 6H 18O 24P 6 into the base solution was helpful in coating formation and the coatings formed in the solution containing 8 g/L C 6H 18O 24P 6 exhibited the best pore uniformity and corrosion resistance.

  14. Chemical nature of phytic acid conversion coating on AZ61 magnesium alloy

    Energy Technology Data Exchange (ETDEWEB)

    Pan Fusheng [College of Materials Science and Engineering, Chongqing University, Chongqing, 400044 (China); Yang Xu, E-mail: yangxu2524@yahoo.com.cn [College of Materials Science and Engineering, Chongqing University, Chongqing, 400044 (China); Chemistry Department, Third Military Medical University, Chongqing, 400038 (China); Zhang Dingfei [College of Materials Science and Engineering, Chongqing University, Chongqing, 400044 (China)

    2009-07-30

    Phytic acid (PA) conversion coating on AZ61 magnesium alloy was prepared by the method of deposition. The influences of pH, time and PA concentration on the formation process, microstructure and properties of the conversion coating were investigated. Scanning electron microscopy (SEM) was used to observe the microstructure. The chemical nature of conversion coating was investigated by energy dispersive X-ray spectroscopy (EDS) and Fourier transform infrared spectroscopy (FTIR) techniques. The corrosion resistance was examined by means of potentiodynamic polarization method. The adhesive ability was tested by score experiments. The results showed that the growth and microstructure of the conversion coatings were all obviously affected by pH, time and PA concentration. In 0.5 mg/ml PA solution with a pH of 5, an optimization conversion coating formed after 20 min immersion time by deposition of PA on AZ61 magnesium alloy surface through chelating with Al{sup 3+}. It made the corrosion potential E{sub corr} of sample shifted positively about 171 mV than that of the untreated sample, and the adhesive ability reached to Grade 1 (in accordance with GB/T 9286).

  15. Identification of Quantitative Trait Loci for Phytic Acid Concentration in Maize Grain Under Two Nitrogen Conditions

    Institute of Scientific and Technical Information of China (English)

    LIU Jian-chao; HUANG Ya-qun; MA Wen-qi; ZHOU Jin-feng; BIAN Fen-ru; CHEN Fan-jun; MI Guo-hua

    2013-01-01

    Phytic acid (PA) is the main storage form of phosphorus (P) in seeds. It can form insoluble complexes with microelements, thereby reducing their bioavailability for animals. Identification of quantitative trait loci (QTLs) associated with grain PA concentration (PAC) is essential to improve this trait without affecting other aspects of grain nutrition such as protein content. Using a recombinant inbred line (RIL) population, we mapped QTL for grain PAC, as well as grain nitrogen concentration (NC) and P concentration (PC) in maize under two N conditions in 2 yr. We detected six QTLs for PAC. The QTL for PAC on chromosome 4 (phi072-umc1276) was identified under both low-N and high-N treatments, and explained 13.2 and 15.4%of the phenotypic variance, respectively. We identified three QTLs for grain NC, none of which were in the same region as the QTLs for PAC. We identified two QTLs for PC in the low-N treatment, one of which (umc1710-umc2197) was in the same interval as the QTL for PAC under high-N conditions. These results suggested that grain PAC can be improved without affecting grain NC and inorganic PC.

  16. Chemical nature of phytic acid conversion coating on AZ61 magnesium alloy

    Science.gov (United States)

    Pan, Fusheng; Yang, Xu; Zhang, Dingfei

    2009-07-01

    Phytic acid (PA) conversion coating on AZ61 magnesium alloy was prepared by the method of deposition. The influences of pH, time and PA concentration on the formation process, microstructure and properties of the conversion coating were investigated. Scanning electron microscopy (SEM) was used to observe the microstructure. The chemical nature of conversion coating was investigated by energy dispersive X-ray spectroscopy (EDS) and Fourier transform infrared spectroscopy (FTIR) techniques. The corrosion resistance was examined by means of potentiodynamic polarization method. The adhesive ability was tested by score experiments. The results showed that the growth and microstructure of the conversion coatings were all obviously affected by pH, time and PA concentration. In 0.5 mg/ml PA solution with a pH of 5, an optimization conversion coating formed after 20 min immersion time by deposition of PA on AZ61 magnesium alloy surface through chelating with Al 3+. It made the corrosion potential Ecorr of sample shifted positively about 171 mV than that of the untreated sample, and the adhesive ability reached to Grade 1 (in accordance with GB/T 9286).

  17. Effect of processing and cooking on mineral and phytic acid content of buckwheat-enriched tagliatelle

    Directory of Open Access Journals (Sweden)

    Jambrec Dubravka

    2016-01-01

    Full Text Available Two types of buckwheat flour - non-treated (NBF and autoclaved (TBF were used for the enrichment of whole wheat tagliatelle (control sample at the level of 10-30% and the produced tagliatelle samples (dry and cooked were examined in terms of mineral and phytic acid (PA content. Both NBF and TBF possessed significantly higher (p < 0.05 content of all investigated minerals compared to whole wheat flour (WWF, but significantly lower (p < 0.05 PA content. Cooked NBF-containing tagliatelle possessed significantly higher (p < 0.05 content of Mg, Zn, Mn and Fe compared to the cooked control sample, while mineral content of cooked TBF-containing tagliatelle samples was not significantly different from the control. Autoclaving significantly reduced (p < 0.05 PA content of cooked TBF-containing tagliatelle samples compared to cooked NBF-containing tagliatelle samples and the control sample. The mineral bioavailability defined through molar ratio of mineral to phytate was slightly improved in buckwheat-containing tagliatelle samples, but it still remains at low level. Regarding all results, the enrichment of whole wheat tagliatelle with NBF at higher levels of substitution (20-30% resulted in significant increase in mineral content and bioavailability.

  18. Genetic modification of low phytic acid 1-1 maize to enhance iron content and bioavailability.

    Science.gov (United States)

    Aluru, Maneesha R; Rodermel, Steve R; Reddy, Manju B

    2011-12-28

    High phytate content in staple food crops is a major barrier to successful iron biofortification. We have exploited the low phytic acid 1-1 (lpa1-1) mutant of maize to generate transgenic plants with up-to 70 μg/g seed iron through the endosperm-specific overexpression of soybean ferritin, resulting in more than 2-fold improvement in iron bioavailability. The levels of bioavailable seed iron achieved in this study greatly exceed any achieved thus far and closely approach values estimated to have a nutritional impact on target populations. Gene expression studies reveal a large induction of the YS1 transporter in leaves and severe repression of an iron acquisition gene DMAS1 in roots, suggesting significant alterations in the iron homeostatic mechanisms in transgenic lpa1-1. Furthermore, preliminary tests show that the high-iron lpa1-1 seeds have higher germination rates and seedling vigor when compared to those of the nontransgenic seeds, which may help improve their value to plant breeders.

  19. Adsorption and Flame Retardant Properties of Bio-Based Phytic Acid on Wool Fabric

    Directory of Open Access Journals (Sweden)

    Xian-Wei Cheng

    2016-04-01

    Full Text Available Bio-based phytic acid (PA as a nontoxic naturally occurring compound is a promising prospect for flame-retardant (FR modifications to polymers. In this work, PA was applied to wool fabric using an exhaustion technique, and the adsorption and FR properties of PA on wool fabric were studied. The flame retardancy of the treated wool fabrics depended greatly on the adsorption quantity of PA, which was related to the pH of treatment solution, immersing temperature and initial PA concentration. The Langmuir adsorption of PA took place due to electrostatic interactions between PA and wool fiber. The limiting oxygen index, vertical burning and pyrolysis combustion flow calorimetry tests revealed that the treated wool fabrics exhibited good flame retardancy. The measurements of the phosphorus content of the burned fabric residues and thermogravimetric analyses suggested that a significant condensed-phase FR action was applicable to the PA treated fabrics. PA treatment was found to have little adverse effect on the whiteness and mechanical performance of wool. Additionally, the washing resistance of the FR fabrics should be further improved.

  20. Difference of Phytic Acid Content and its Relation to Four Protein Composition Contents in Grains of Twenty-nine japonica Rice Varieties from Jiangsu and Zhejiang Provinces, China

    Institute of Scientific and Technical Information of China (English)

    WU Wei; CHENG Fang-min; LIU Zheng-hui; WEI Ke-su

    2007-01-01

    Twenty-nine japonica rice varieties collected from Jiangsu and Zhejiang Provinces, China were planted in Hangzhou,China, to investigate the phytic acid content in brown rice and its frequency distribution as well as the correlation among the contents of phytic acid, total protein and four protein compositions in brown rice. The phytic acid content in brown rice ranged from 0.699% to 1.034%, with a mean of 0.868% for the 29 tested rice varieties. Xiushui series rice varieties generally exhibited lower phytic acid level than Wuyujing and Huai series rice varieties. A rough normal distribution, with a mean of 8.722%, was observed for the total protein contents in the tested varieties. Of the four protein compositions, the glutelin, globulin and albumin contents had larger coefficient of variation than the prolamin content, although the difference in prolamin content was genotype-dependent. No significant correlation was found between the phytic acid and four protein composition contents, whereas the total protein content was significantly and positively related to the glutelin content in brown rice.

  1. Effect of phytic acid etchant on the structural stability of demineralized dentine and dentine bonding.

    Science.gov (United States)

    Kong, Kalyan; Islam, Md Sofiqul; Nassar, Mohannad; Hiraishi, Noriko; Otsuki, Masayuki; Yiu, Cynthia K Y; Tagami, Junji

    2015-08-01

    This study examined the effect of phytic acid (IP6) in stabilizing the morphology of dentine collagen network and resin-dentine bonding. Dentine beams were fully demineralized with 10% phosphoric acid (PA) or 1% IP6 (pH 1.2). PA-demineralized beams were divided into three groups: (a) no further treatment (control), (b) treatment with 5% glutaraldehyde (GA) for 1 h and (c) treatment with 1% IP6 (pH 7) for 1 h. IP6-demineralized beams received no further treatment. The beams were then subjected to ultimate tensile strength (UTS) testing. Dentine micromorphology evaluation was performed using a field-emission scanning electron microscope (FE-SEM). Dentine disks were etched with 35% PA for 15 s or 1% IP6 for 30s. PA-etched dentine disks were divided into three groups as (a), (b) and (c) as for UTS testing, but the treatment with GA or IP6 was done in 1min. For microtensile bond strength (µTBS) testing, flat dentine surfaces etched with PA or IP6 were blot-dried (wet dentine) or air-dried for 10s (dry dentine) and bonded with an etch-and-rinse adhesive followed by composite build-up. IP6-demineralized dentine showed significantly higher UTS, when compared to PA-demineralized dentine. GA and IP6 significantly improved UTS of PA-demineralized dentine. FE-SEM observation revealed that dentine collagen network was preserved by GA and IP6. No significant difference in µTBS was found between the wet and dry IP6-etched dentine groups. IP6 etching showed a structural stabilizing effect on demineralized dentine matrix and produced good resin-dentine bonding, regardless of dentine moistness or dryness. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. Zinc absorption from low phytic acid genotypes of maize (Zea mays L.), Barley (Hordeum vulgare L.), and Rice (Oryza sativa L.) assessed in a suckling rat pup model.

    Science.gov (United States)

    Lönnerdal, Bo; Mendoza, Concepcion; Brown, Kenneth H; Rutger, J Neil; Raboy, Victor

    2011-05-11

    Dietary phytic acid is a major causative factor for low Zn bioavailability in many cereal- and legume-based diets. The bioavailability of Zn in seed of low phytic acid (lpa) variants of maize ( Zea mays L.), rice ( Oryza sativa L.), and barley ( Hordeum vulgare L.) was evaluated using a suckling rat pup model. Suckling rat pups (14 days old, n = 6-8/treatment) were fasted for 6 h and intubated with (65)Zn-radiolabeled suspensions prepared using seed produced by either wild-type (normal phytic acid) or lpa genotypes of each cereal. Test solutions were radiolabeled overnight (all genotypes) or immediately prior to intubation (barley genotypes). Pups were killed 6 h postintubation and tissues removed and counted in a gamma counter. Zn absorption was low from wild-type genotypes of maize (21, 33%) and rice (26%), and phytic acid reduction resulted in significantly higher Zn absorption, 47-52 and 35-52%, respectively. Zn absorption from wild-type barley incubated overnight was high (86-91%), and phytate reduction did not improve Zn absorption (84-90%), which is likely due to endogenous phytase activity. When the wild-type barley solutions were prepared immediately before intubation, Zn absorption was significantly lower (63, 78%) than from the lpa cultivars (92, 96%). Variation in seed or flour phenolic acid levels did not affect Zn absorption. Differences in seed Zn levels did not substantially affect Zn absorption. Thus, when phytic acid is abundant in a diet, it has a larger effect on Zn absorption than the level of Zn. Therefore, reducing the phytic acid content of staple cereal grains may contribute to enhancing Zn nutrition of populations consuming these staple foods.

  3. Enhanced proton conductivity of Nafion hybrid membrane under different humidities by incorporating metal-organic frameworks with high phytic acid loading.

    Science.gov (United States)

    Li, Zhen; He, Guangwei; Zhang, Bei; Cao, Ying; Wu, Hong; Jiang, Zhongyi; Tiantian, Zhou

    2014-06-25

    In this study, phytic acid (myo-inositol hexaphosphonic acid) was first immobilized by MIL101 via vacuum-assisted impregnation method. The obtained phytic@MIL101 was then utilized as a novel filler to incorporate into Nafion to fabricate hybrid proton exchange membrane for application in PEMFC under different relative humidities (RHs), especially under low RHs. High loading and uniform dispersion of phytic acid in MIL 101(Cr) were achieved as demonstrated by ICP, FT-IR, XPS, and EDS-mapping. The phytic@MIL101 was dispersed homogeneously in the Nafion matrix when the filler content was less than 12%. Hybrid membranes were evaluated by proton conductivity, mechanical property, thermal stability, and so forth. Remarkably, the Nafion/phytic@MIL hybrid membranes showed high proton conductivity at different RHs, especially under low RHs, which was up to 0.0608 S cm(-1) and 7.63 × 10(-4) S cm(-1) at 57.4% RH and 10.5% RH (2.8 and 11.0 times higher than that of pristine membrane), respectively. Moreover, the mechanical property of Nafion/phtic@MIL hybrid membranes was substantially enhanced and the thermal stability of membranes was well preserved.

  4. Genotype x Environment interaction for antioxidants and phytic acid contents in bread and durum wheat as influenced by climate

    Directory of Open Access Journals (Sweden)

    Gordana Brankovic

    2015-06-01

    Full Text Available Antioxidants prevent oxidative stress and exert positive health effects. However, phytic acid among them decreases micronutrients absorption, representing also antinutrient to human and non-ruminant animals. Fifteen bread wheat (Triticum aestivum L. and 15 durum wheat (Triticum durum Desf. genotypes were evaluated across six environments to determine contents of phytic acid (PA, inorganic P (Pi, total yellow pigment, total soluble phenolic compounds, free protein sulfhydryl groups (PSH, and also phytic acid P/Pi (Pp/Pi. The objective of this study was to quantify, for each trait the effects of environment, genotype, and their interaction; and the influence of climatic factors on the Genotype x Environment interaction (GEI by the use of the factorial regression. GEI (P < 0.001 prevailed as source of variation over genotype (P < 0.001 in determining PA content in bread and durum wheat (44.3% and 34.7% of sum of squares-SS, respectively, PSH content in bread and durum wheat (27% and 28.4% of SS, respectively and total soluble phenolic compounds content in durum wheat (35.5% of SS. The major contribution to the GEI represented climatic variables during stages of stem elongation for PA and phenolic compounds, and also flowering, fertilization, grain formation and grain filling for PSH. Total yellow pigment and Pi contents in bread and durum wheat were predominantly determined by genotype (P < 0.001. Models of climatic variables proved to be efficient in the explanation of more than 92% of the SS of GEI for PA and antioxidants contents.

  5. Influence of phytic acid concentration on coating properties obtained by MAO treatment on magnesium alloys

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, R.F., E-mail: rfzhang-10@163.com [Jiangxi Key Laboratory of Surface Engineering, Jiangxi Science and Technology Normal University, Nanchang 330013 (China) and School of Material Science and Engineering, Jiangxi Science and Technology Normal University, Nanchang 330013 (China); Zhang, S.F.; Duo, S.W. [Jiangxi Key Laboratory of Surface Engineering, Jiangxi Science and Technology Normal University, Nanchang 330013 (China); School of Material Science and Engineering, Jiangxi Science and Technology Normal University, Nanchang 330013 (China)

    2009-06-30

    Anodic coatings were prepared by microarc oxidation (MAO) on AZ91HP in a base solution of 10 g/L NaOH with and without the addition of 0-12 g/L phytic acid (C{sub 6}H{sub 18}O{sub 24}P{sub 6}). The influences of C{sub 6}H{sub 18}O{sub 24}P{sub 6} and its concentration on the conductivity and breakdown voltage were studied. The morphologies and compositions of anodic coatings were determined by environmental scanning electron microscope (ESEM) and energy dispersive X-ray spectroscopy (EDX). Potentiodynamic polarization test was performed in 3.5 wt.% NaCl solution to evaluate the corrosion resistance of anodic coatings. The results showed that with the increase of C{sub 6}H{sub 18}O{sub 24}P{sub 6} concentration, the solution conductivity decreased while the values of breakdown voltage increased. EDX analysis showed that the coatings formed in solutions with C{sub 6}H{sub 18}O{sub 24}P{sub 6} addition contained Mg, Al, O, C, P and a trance of Na. The addition of C{sub 6}H{sub 18}O{sub 24}P{sub 6} into the base solution was helpful in coating formation and the coatings formed in the solution containing 8 g/L C{sub 6}H{sub 18}O{sub 24}P{sub 6} exhibited the best pore uniformity and corrosion resistance.

  6. Polyacrylamide-Phytic Acid-Polydopamine Conducting Porous Hydrogel for Efficient Removal of Water-Soluble Dyes

    OpenAIRE

    Zhao, Zhen; Li, Leijiao; Geleta, Girma Selale; Ma, Lina; Wang, Zhenxin

    2017-01-01

    Removal of toxic dyes from wastewater has become a hot topic in both academic and industrial fields since there is growing concern about the threat of sewage to human health. Herein, we demonstrate that the three-dimensional porous polyacrylamide-phytic acid-polydopamine (termed as PAAM/PA/PDA) hydrogel can be served as reusable adsorbent with high efficiency for either anionic or cationic dyes. Using methyl blue (MB), methylene blue (YMB), methyl violet (MV) and neutral red (NR) as model dye...

  7. Biochemical and molecular characterization of a mutation that confers a decreased raffinosaccharide and phytic acid phenotype on soybean seeds.

    Science.gov (United States)

    Hitz, William D; Carlson, Thomas J; Kerr, Phil S; Sebastian, Scott A

    2002-02-01

    A single, recessive mutation in soybean (Glycine max L. Merr.), which confers a seed phenotype of increased inorganic phosphate, decreased phytic acid, and a decrease in total raffinosaccharides, has been previously disclosed (S.A. Sebastian, P.S. Kerr, R.W. Pearlstein, W.D. Hitz [2000] Soy in Animal Nutrition, pp 56-74). The genetic lesion causing the multiple changes in seed phenotype is a single base change in the third base of the codon for what is amino acid residue 396 of the mature peptide encoding a seed-expressed myo-inositol 1-phospate synthase gene. The base change causes residue 396 to change from lysine to asparagine. That amino acid change decreases the specific activity of the seed-expressed myo-inositol 1-phosphate synthase by about 90%. Radio tracer experiments indicate that the supply of myo-inositol to the reaction, which converts UDP-galactose and myo-inositol to galactinol is a controlling factor in the conversion of total carbohydrate into the raffinosaccharides in both wild-type and mutant lines. That same decrease in myo-inositol 1-phosphate synthetic capacity leads to a decreased capacity for the synthesis of myo-inositol hexaphosphate (phytic acid) and a concomitant increase in inorganic phosphate.

  8. Effects of two low phytic acid mutations on seed quality and nutritional traits in soybean (Glycine max L. Merr).

    Science.gov (United States)

    Yuan, Feng-Jie; Zhu, Dan-Hua; Deng, Bo; Fu, Xu-Jun; Dong, De-Kun; Zhu, Shen-Long; Li, Bai-Quan; Shu, Qing-Yao

    2009-05-13

    Reduction of phytic acid in soybean seeds has the potential to improve the nutritional value of soybean meal and lessen phosphorus pollution in large scale animal farming. The objective of this study was to assess the effect of two new low phytic acid (LPA) mutations on seed quality and nutritional traits. Multilocation/season comparative analyses showed that the two mutations did not affect the concentration of crude protein, any of the individual amino acids, crude oil, and individual saturated fatty acids. Among other traits, Gm-lpa-TW75-1 had consistently higher sucrose contents (+47.4-86.1%) and lower raffinose contents (-74.2 to -84.3%) than those of wild type (WT) parent Taiwan 75; Gm-lpa-ZC-2 had higher total isoflavone contents (3038.8-4305.4 microg/g) than its parent Zhechun # 3 (1583.6-2644.9 microg/g) in all environments. Further tests of homozygous F(3) progenies of the cross Gm-lpa-ZC-2 x Wuxing # 4 (WT variety) showed that LPA lines had a mean content of total isoflavone significantly higher than WT lines. This study demonstrated that two LPA mutant genes have no negative effects on seed quality and nutritional traits; they instead have the potential to improve a few other properties. Therefore, these two mutant genes are valuable genetic resources for breeding high quality soybean varieties.

  9. A survey of the agronomic and end-use characteristics of low phytic acid soybeans

    Science.gov (United States)

    With unique high protein and oil contents, soybean (Glycine max L. merr.) is one of the most widely grown agronomic crops in the United States. Around 98% of those soybeans are used in animal feeds ranging from swine and cattle to domestic animals and aquaculture. This chapter will introduce phytic ...

  10. Avaliação da atividade antioxidante do ácido fítico de germe de milho Corn germ phytic acid antioxidant activity evaluation

    Directory of Open Access Journals (Sweden)

    Cristina Tostes Filgueiras

    2009-01-01

    Full Text Available The obtained corn germ phytic acid (CGPA antioxidant potential was evaluated through the deoxyribose, bathophenanthroline (BPS and DPPH• assays. In the concentration of 130.5 μM of CGPA the hydroxyl radical maximum sequestering antioxidant activity was 29.3% while standard phytic acid (SPA presented this maximum activity of 18.2% in the concentration of 33.2 μM of SPA. The BPS assays revealed that the chelation activity towards Fe2+ increased concurrently with the increase of CGPA concentration and its Fe2+ contact time. Finally, DPPH• assay showed that CGPA and SPA did not present electron-donating capacity to DPPH•.

  11. A carbon dot-based "off-on" fluorescent probe for highly selective and sensitive detection of phytic acid.

    Science.gov (United States)

    Gao, Zhao; Wang, Libing; Su, Rongxin; Huang, Renliang; Qi, Wei; He, Zhimin

    2015-08-15

    We herein report a facile, one-step pyrolysis synthesis of photoluminescent carbon dots (CDs) using citric acid as the carbon source and lysine as the surface passivation reagent. The as-prepared CDs show narrow size distribution, excellent blue fluorescence and good photo-stability and water dispersivity. The fluorescence of the CDs was found to be effectively quenched by ferric (Fe(III)) ions with high selectivity via a photo-induced electron transfer (PET) process. Upon addition of phytic acid (PA) to the CDs/Fe(III) complex dispersion, the fluorescence of the CDs was significantly recovered, arising from the release of Fe(III) ions from the CDs/Fe(III) complex because PA has a higher affinity for Fe(III) ions compared to CDs. Furthermore, we developed an "off-on" fluorescence assay method for the detection of phytic acid using CDs/Fe(III) as a fluorescent probe. This probe enables the selective detection of PA with a linear range of 0.68-18.69 μM and a limit of detection (signal-to-noise ratio is 3) of 0.36 μM. The assay method demonstrates high selectivity, repeatability, stability and recovery ratio in the detection of the standard and real PA samples. We believe that the facile operation, low-cost, high sensitivity and selectivity render this CD-based "off-on" fluorescent probe an ideal sensing platform for the detection of PA.

  12. Validation study of a rapid colorimetric method for the determination of phytic acid and inorganic phosphorus from seeds

    Directory of Open Access Journals (Sweden)

    Dragičević Vesna D.

    2011-01-01

    Full Text Available Phytate, as an important mineral storage compound in seeds, is vital for seed/grain development; it is often considered to be an antinutritional substance. The objective of this study was to develop a rapid and inexpensive colorimetric method of measuring phytate and inorganic P (Pi concentrations from maize, soybean and sunflower seed/grain extracts, by combining adequate precision and simplicity, ideal for breeders interested in improving simultaneously Pi and phytate levels. The investigated extraction mediums: double distilled (DD H2O, 2.4 % HCl and 5 % trichloracetic acid (TCA were proved to be suitable for the analysis of phytic acid and inorganic phosphorus in seed extracts. The advantages of 5 % TCA over to DD H2O and 2.4 % HCl were reflected through the low limit of detection for both phytic acid and Pi and good recovery with low bias. A low detection limit for Pi is important for samples with naturally low Pi concentrations, such as soybean seeds.

  13. Equilibrium, thermoanalytical and spectroscopic studies to characterize phytic acid complexes with Mn(II) and Co(II)

    Energy Technology Data Exchange (ETDEWEB)

    Carli, Ligia de; Schnitzler, Egon; Rosso, Neiva Deliberali [Universidade Estadual de Ponta Grossa (UEPG), PR (Brazil). Dept. de Quimica], e-mail: ndrosso@uepg.br; Ionashiro, Massao [Universidade Estadual Paulista (UNESP), Araraquara, SP (Brazil). Inst. de Quimica; Szpoganicz, Bruno [Universidade Federal de Santa Catarina (UFSC), Florianopolis, SC (Brazil). Dept. de Quimica

    2009-07-01

    Potentiometric studies were carried out to determine the binding degree of phytic acid with Co(II) and Mn(II) ions, in the absence of dioxygen. Equilibrium constants for all major complexes formed are reported, and the results are presented in the form of distribution diagrams showing the concentrations of individual complexes as a function of pH. The formation constants of the complexes show higher values for the species in which the ligand was more deprotonated. Potentiometric data indicates that the species [MH{sub 4}L]{sup 6-}, was totally formed at pH 7.0 and the complexes were synthesized from this data. A solid state complex of Mn(II) and Co(II) with phytic acid was synthesized. Thermogravimetry, differential scanning calorimetry, and infrared spectroscopy were used to investigate and characterize the thermal behavior of these compounds. The results led to information on the composition, dehydration, thermal stability and thermal decomposition of the isolated complexes. (author)

  14. Strategies to optimize biosensors based on impedance spectroscopy to detect phytic acid using layer-by-layer films.

    Science.gov (United States)

    Moraes, Marli L; Maki, Rafael M; Paulovich, Fernando V; Rodrigues Filho, Ubirajara P; de Oliveira, Maria Cristina F; Riul, Antonio; de Souza, Nara C; Ferreira, Marystela; Gomes, Henrique L; Oliveira, Osvaldo N

    2010-04-15

    Impedance spectroscopy has been proven a powerful tool for reaching high sensitivity in sensor arrays made with nanostructured films in the so-called electronic tongue systems, whose distinguishing ability may be enhanced with sensing units capable of molecular recognition. In this study we show that for optimized sensors and biosensors the dielectric relaxation processes involved in impedance measurements should also be considered, in addition to an adequate choice of sensing materials. We used sensing units made from layer-by-layer (LbL) films with alternating layers of the polyeletrolytes, poly(allylamine) hydrochloride (PAH) and poly(vinyl sulfonate) (PVS), or LbL films of PAH alternated with layers of the enzyme phytase, all adsorbed on gold interdigitate electrodes. Surprisingly, the detection of phytic acid was as effective in the PVS/PAH sensing system as with the PAH/phytase system, in spite of the specific interactions of the latter. This was attributed to the dependence of the relaxation processes on nonspecific interactions such as electrostatic cross-linking and possibly on the distinct film architecture as the phytase layers were found to grow as columns on the LbL film, in contrast to the molecularly thin PAH/PVS films. Using projection techniques, we were able to detect phytic acid at the micromolar level with either of the sensing units in a data analysis procedure that allows for further optimization.

  15. Barley (Hordeum vulgare L.) low phytic acid 1-1: an endosperm-specific, filial determinant of seed total phosphorus.

    Science.gov (United States)

    Raboy, Victor; Cichy, Karen; Peterson, Kevin; Reichman, Sarah; Sompong, Utumporn; Srinives, Peerasak; Saneoka, Hirofumi

    2014-01-01

    Inositol hexaphosphate (Ins P6 or "phytic acid") typically accounts for 75 (± 10%) of seed total phosphorus (P). In some cases, genetic blocks in seed Ins P6 accumulation can also alter the distribution or total amount of seed P. In nonmutant barley (Hordeum vulgare L.) caryopses, ~80% of Ins P6 and total P accumulate in the aleurone layer, the outer layer of the endosperm, with the remainder in the germ. In barley low phytic acid 1-1 (Hvlpa1-1) seed, both endosperm Ins P6 and total P are reduced (~45% and ~25%, respectively), but germs are phenotypically wild type. This translates into a net reduction in whole-seed total P of ~15%. Nutrient culture studies demonstrate that the reduction in endosperm total P is not due to a reduction in the uptake of P into the maternal plant. Genetic tests (analyses of testcross and F2 seed) reveal that the Hvlpa1-1 genotype of the filial seed conditions the seed total P reduction; sibling seed in the same head of barley that differ in their Hvlpa1-1 genotype (heterozygous vs. homozygous recessive) differ in their total P (normal vs. reduced, respectively). Therefore, Hvlpa1 functions as a seed-specific or filial determinant of barley endosperm total P.

  16. Enhanced visualization of small peptides absorbed in rat small intestine by phytic-acid-aided matrix-assisted laser desorption/ionization-imaging mass spectrometry.

    Science.gov (United States)

    Hong, Seong-Min; Tanaka, Mitsuru; Yoshii, Saori; Mine, Yoshinori; Matsui, Toshiro

    2013-11-05

    Enhanced visualization of small peptides absorbed through a rat intestinal membrane was achieved by matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry (MALDI-IMS) with the aid of phytic acid as a matrix additive. Penetrants through intestinal peptide transporter 1, i.e., glycyl-sarcosine (Gly-Sar, 147.1 m/z) and antihypertensive dipeptide, Val-Tyr (281.2 m/z), were chosen for MALDI-IMS. The signal-to-noise (S/N) ratios of dipeptides Gly-Sar and Val-Tyr were seen to increase by 2.4- and 8.0-fold, respectively, when using a 2',4',6'-trihydroxyacetophenone (THAP) matrix containing 5.0 mM phytic acid, instead of the THAP matrix alone. Owing to the phytic-acid-aided MALDI-IMS method, Gly-Sar and Val-Tyr absorbed in the rat intestinal membrane were successfully visualized. The proposed imaging method also provided useful information on intestinal peptide absorption; to some extent, Val-Tyr was rapidly hydrolyzed to Tyr by peptidases located at the intestinal microvillus during the absorption process. In conclusion, the strongly acidic additive, phytic acid, is beneficial for enhancing the visualization of small peptides using MALDI-IMS, owing to the suppression of ionization-interfering salts in the tissue.

  17. Study on ultrasonically enhanced extraction of phytic acid from rice bran%超声波强化提取米糠中植酸研究

    Institute of Scientific and Technical Information of China (English)

    胡爱军; 田玲玲; 郑捷; 孙连立; 王豪

    2012-01-01

      As a native food additive,phytic acid has strong anti–corrosive and antioxidative properties. With rice bran as raw materials,ultrasound was used to improve the extraction yield of phytic acid. The influence of some factors on the extraction yield of phytic acid were investigated,including ultrasonication time,extraction temperature,the ratio of raw material to solvent and hydrochloric acid concentration. and the orthogonal experiment was conducted to optimize the extraction process using HPLC to analyze phytic acid. The results showed that the optimal extraction conditions were ultrasonication time at 8 min,extraction temperature at 40℃,the ratio of raw material to solven at 1∶8 and concentration hydrochloric acid at 0.10 mol/L,under above conditions,the extraction yield of phytic acid was 87.13%.%  植酸是一种天然食品添加剂,具有强烈防腐、抗氧化能力.该实验以米糠为原料,利用超声波强化提取植酸,研究超声时间、提取温度、料液比及盐酸浓度对植酸提取率影响;通过正交试验对提取工艺进行优化,并采用高效液相色谱法对植酸样品进行分析.结果表明,提取米糠中植酸最优工艺为:盐酸浓度0.10 mol/L、料液比(g/mL)1∶8、提取温40℃、超声时间8 min;在上述条件下,对米糠中植酸提取率为87.13%.

  18. Gastric digestion of pea ferritin and modulation of its iron bioavailability by ascorbic and phytic acids in caco-2 cells

    Institute of Scientific and Technical Information of China (English)

    Satyanarayana Bejjani; Raghu Pullakhandam; Ravinder Punjal; K Madhavan Nair

    2007-01-01

    AIM: To understand the digestive stability and mechanism of release and intestinal uptake of pea ferritin iron in caco-2 cell line model.METHODS: Pea seed ferritin was purified using salt fractionation followed by gel filtration chromatography.The bioavailability of ferritin iron was assessed using coupled in vitro digestion/Caco-2 cell model in the presence or absence of ascorbic acid and phytic acid.Caco-2 cell ferritin formation was used as a surrogate marker of iron uptake. Structural changes of pea ferritin under simulated gastric pH were characterized using electrophoresis, gel filtration and circular dichroism spectroscopy.RESULTS: The caco-2 cell ferritin formation was significantly increased (P < 0.001) with FeSO4 (19.3±9.8 ng/mg protein) and pea ferritin (13.9 ± 6.19 ng/mg protein) compared to the blank digest (3.7 ± 1.8 ng/mg protein). Ascorbic acid enhanced while phytic acid decreased the pea ferritin iron bioavailability. However,either in the presence or absence of ascorbic acid, the ferritin content of caco-2 cells was significantly less with pea ferritin than with FeSO4. At gastric pH, no band corresponding to ferritin was observed in the presence of pepsin either on native PAGE or SDS-PAGE. Gel filtration chromatography and circular dichroism spectroscopy revealed a pH dependent loss of quaternary and secondary structure.CONCLUSION: Under gastric conditions, the iron core of pea ferritin is released into the digestive medium due to acid induced structural alterations and dissociation of protein. The released iron interacts with dietary factors leading to modulation of pea ferritin iron bioavailability,resembling the typical characteristics of non-heme iron.

  19. Generation of stable 'low phytic acid' transgenic rice through antisense repression of the 1D-myo-inositol 3-phosphate synthase gene (RINO1) using the 18-kDa oleosin promoter.

    Science.gov (United States)

    Kuwano, Mio; Mimura, Tetsuro; Takaiwa, Fumio; Yoshida, Kaoru T

    2009-01-01

    Phytic acid acts as the major storage form of phosphorus in plant seeds and is poorly digested by monogastric animals. The degradation of phytic acid in animal diets is necessary to overcome both environmental and nutritional issues. The enzyme 1D-myo-inositol 3-phosphate [Ins(3)P(1)] synthase (EC 5.5.1.4) catalyses the first step of myo-inositol biosynthesis and directs phytic acid biosynthesis in seeds. The rice Ins(3)P(1) synthase gene (RINO1) is highly expressed in developing seed embryos and in the aleurone layer, where phytic acid is synthesized and stored. In rice seeds, 18-kDa oleosin (Ole18) is expressed in a seed-specific manner, and its transcripts are restricted to the embryo and the aleurone layer. Therefore, to effectively suppress phytic acid biosynthesis, antisense RINO1 cDNA was expressed under the control of the Ole18 promoter, directing the same spatial pattern in seeds as RINO1 in transgenic rice plants. The generated transgenic rice plants showed strong 'low phytic acid' (lpa) phenotypes, in which seed phytic acid was reduced by 68% and free available phosphate was concomitantly increased. No negative effects on seed weight, germination or plant growth were observed. The available phosphate levels of the stable transgenic plants surpassed those of currently available rice lpa mutants.

  20. Effect of fermentation with Saccharomyces cerevisiae strain PJ69-4 on the phytic acid, raffinose, and stachyose contents of soybean meal

    Science.gov (United States)

    Three experiments were conducted to determine the impact of submerged fermentation procedures using Saccharomyces cerevisiae baker’s yeast strain PJ69-4a on degradation of phytic acid and the raffinosaccharides, raffinose, and stachyose, in soybean meal. The goal of the research was to identify a n...

  1. The site of net absorption of Ca from the intestinal tract of growing pigs and effect of phytic acid, Ca level and Ca source on Ca digestibility.

    Science.gov (United States)

    González-Vega, J Caroline; Walk, Carrie L; Liu, Yanhong; Stein, Hans H

    2014-01-01

    An experiment was conducted to test the hypothesis that the standardised digestibility of Ca in calcium carbonate and Lithothamnium calcareum Ca is not different regardless of the level of dietary Ca, and that phytic acid affects the digestibility of Ca in these two ingredients to the same degree. The objectives were to determine where in the intestinal tract Ca absorption takes place and if there are measurable quantities of basal endogenous Ca fluxes in the stomach, small intestine or large intestine. Diets contained calcium carbonate or L. calcareum Ca as the sole source of Ca, 0% or 1% phytic acid and 0.4% or 0.8% Ca. A Ca-free diet was also formulated and used to measure endogenous fluxes and losses of Ca. Nine growing pigs (initial body weight 23.8 ± 1.3 kg) were cannulated in the duodenum and in the distal ileum, and faecal, ileal and duodenal samples were collected. Duodenal endogenous fluxes of Ca were greater (p phytic acid, but decreased (p phytic acid, but may be affected by dietary Ca level depending on the Ca source. Calcium from calcium carbonate is mostly absorbed before the duodenum, but Ca from L. calcareum Ca is mostly absorbed in the jejunum and ileum.

  2. A substantial fraction of barley (Hordeum vulgare L.) low phytic acid mutations have little or no effect on yield across diverse production environments

    Science.gov (United States)

    The potential benefits of the low phytic acid (lpa) seed trait for human and animal nutrition, and for phosphorus management in non-ruminant animal production, are well documented. However, in many cases the lpa trait is associated with impaired seed or plant performance, resulting in reduced yield....

  3. Preparation of conversion coating on Ti-6Al-4V alloy in mixed solution of phytic acid and ammonium fluoride through chemical modification

    Science.gov (United States)

    Li, Lanlan; He, Jian; Yang, Xu

    2016-05-01

    Conversion coatings on Ti-6Al-4V alloy was prepared through chemical modification in phytic acid and ammonium fluoride mixed solution. The influences of pH, time and the composition of solution on the microstructure of alloy surface were investigated. Scanning electron microscopy was used to observe the microstructure. The chemical composition of alloy surface before and after modification was investigated by energy dispersive X-ray spectroscopy. The results indicated that a conversion coating could be formed on the Ti-6Al-4V alloy in a mixed solution of phytic acid and ammonium fluoride, the growth and microstructure of the conversion coatings were critically dependent on the pH, time and concentration of phytic acid and ammonium fluoride. In 100 mg/ml phytic acid containing 125 mg/ml ammonium fluoride solution with a pH of 6, a compact conversion coating with the thickness of about 4.7 μm formed after 30 min immersion on Ti-6Al-4V alloy surface. The preliminary evaluation of bioactivity of conversion coating was performed by in vitro cell experiments. The results showed that this chemical modification method is a promising surface modification technique for Ti-6Al-4V alloy inplants.

  4. Optimization of Synthesis Process for Phytic Acid by Response Surface Methodology%响应面分析法优化植酸合成工艺

    Institute of Scientific and Technical Information of China (English)

    刘倩倩

    2015-01-01

    Phytic acid was synthesized with inositol as raw materal and phosphorus pentoxide as the phosphating agent through phosphorization;synthetise;decolorization and filtration. Based on single factor tests ,the optimum synthesis conditions of phytic acid were obtained through Box-Benhnken central combination design and RSM. The results showed that the optimum synthesis conditions of phytic acid were as follows:molar ratio of waterto phosphorus pentoxide 2.1, reaction temperature 174℃, reaction time 3.3 h, the optimized yield of phytic acid was 80.32%.%采用肌醇为原料,以P2O5为磷化剂,通过磷化、合成、脱色、过滤制备植酸.在单因素试验的基础上,根据中心组合(Box-Benhnken)试验设计原理采用三因素三水平的响应面分析法优化植酸合成工艺条件,在分析各个因素的显著性和交互作用后,得到植酸合成最佳工艺条件:H2O与P2O5的反应摩尔比为2.1,合成温度174℃,合成时间3.3h,其植酸含量最高为80.32%.

  5. IMPACT OF GENISTEIN AND PHYTIC ACID ON THE VIABILITY AND PROLIFERATION ACTIVITY OF NASAL POLYPS' CELLS IN AN IN VITRO MODEL.

    Science.gov (United States)

    Frączek, Marcin; Kuśmierz, Dariusz; Rostkowska-Nadolska, Beata; Kręcicki, Tomasz; Latocha, Małgorzata T

    2015-01-01

    In developed countries, chronic rhinosinusitis with nasal polyps is one of the diseases that diminish patients' quality of life most significantly. Treatment of that often incurable disease is based on the steroids and surgery in patients who had failed thorough conservative management. It appears that the introduction of new treatment agents suppressing inflammation process and inhibiting cells' proliferation would be a valuable therapeutic option. The aim of the present study was to evaluate the in vitro effect of genistein and phytic acid on the viability and growth rate of fibroblasts derived from nasal polyps. Cells were incubated with various concentrations of genistein (5-500 μM) and phytic acid (100-20,000 μM). After 72 h incubation, cells survivability and cells' growth rate were estimated by combination of WST-1 and LDH methods. QRT-PCR technique was used to determine the expression of histone H3, BCL-2, BAX and P53 genes. Caspase-8 and -9 expressions were evaluated by ELISA assay. Genistein and phytic acid significantly and in dose-specific manner decreased nasal polyps fibroblasts survivability and growth rate. Both agents in similar way decreased cell proliferation as measured by the expression of histone H3. They induce apoptotic machinery by modulating the expression of BCL-2, BAX and caspase-8 activity. Genistein and phytic acid have significant potential for a therapeutic role in the treatment of chronic rhinosinusitis.

  6. Comparison of the Phosphorus and Mineral Concentrations in Bran and Abraded Kernel Fractions of a Normal Barley (Hordeum vulgare) Cultivar versus Four Low Phytic Acid Isolines

    Science.gov (United States)

    Phytic acid consists of 65-80% of the total phosphorus (P) in cereal grains. Its salts are concentrated in the germ and aleurone layers, which are typically removed during milling. We hypothesize that concentrations of different types of P and minerals in milled products will be greatly altered in ...

  7. Antimicrobial mechanisms of phytic acid against Escherichia coli%植酸对大肠杆菌抑菌机理的研究

    Institute of Scientific and Technical Information of China (English)

    侯伟峰; 谢晶; 蓝蔚青; 朱军伟

    2012-01-01

    To identify the antimicrobial mechanisms of phytic acid against Escherichia coli, inhibition effects, minimum inhibitory concentration (MIC), bacterial growth curve, alkaline phosphatase(/4KP) content, and electric conductivity of the bacteria were determined, and the cell ultrastracture was observed. The results showed that phytic acid displayed a strong inhibitory effect against the bacteria, and the higher the concentration of phytic acid, the stronger the inhibition. The MIC (volume fraction) of Escherichia coli was 0. 4%. Compared with the control (no phytic acid) , phytic acid changed bacterial growth curve, destroyed the cells seriously. Permeability of cell membrane and wall were increased, and cell components were released, then AKP and electric conductivity were increased.%为了研究植酸对大肠杆菌的抑菌机理,测定了植酸对大肠杆菌的抑菌效果、最小抑菌浓度、细菌生长曲线、菌液碱性磷酸酶(AKP)含量及电导率,并观察了细菌超微结构.结果表明,植酸对大肠杆菌有较强的抑菌效果,且随着浓度的增大而增强,植酸对大肠杆菌的最低抑菌浓度(体积分数)为0.4%;与不添加植酸的对照相比,植酸改变了大肠杆菌的生长规律,使细胞破损严重,细胞壁和细胞膜通透性增加,细胞质外渗,菌液中AKP含量和电导率增大.

  8. 植酸对腐败希瓦氏菌的抑菌机理%Antimicrobial mechanisms of phytic acid against Shewanella putrefacens

    Institute of Scientific and Technical Information of China (English)

    谢晶; 侯伟峰; 汤毅; 蓝蔚青

    2011-01-01

    结合植酸对南美白对虾的保鲜,以虾体优势腐败菌一腐败希瓦氏菌为研究对象,利用植酸溶液处理后,通过对细菌的抑菌效果及最小抑菌浓度、细菌生长曲线、碱性磷酸酶(AKP)等的测定研究其抑菌机理。结果表明,植酸对腐败希瓦氏菌有较强的抑菌效果,最低抑菌浓度(体积分数)为0.2%。同对照组相比,植酸能够影响细菌的生长规律,使细胞破损。细胞壁和细胞膜遭到破坏,AKP及电导率均增大,初步阐述了植酸对腐败希瓦氏菌的抑菌机理。%Combined with phytic acid on the preservation of Penaeus Vanmarnei, advantage corruption bacteria of Penaeus Vanmamei - Shewanella putrefacens was selected as the sample to investigate the antimicrobial mechanisms of phytic acid by inhibition effects,minimum inhibitory concentration (MIC),bacterial curve,alkaline phosphatase (AKP) and so on. Results showed that phytic acid had a strong inhibition function against the bacteria,the MIC (volume fraction) of Shewanella putrefacens was 0.2%. Compared with the controlled,phytic acid can change bacterial curve,destroyed the cells. Cell membrane and wall were destroyed,then,AKP and electric conductivity were increased. The antimicrobial mechanisms of phytic acid against Shewanella putrefacens were proposed.

  9. Phytic acid activation prior to electroless nickel plating on magnesium alloy%镁合金化学镀镍前植酸活化工艺

    Institute of Scientific and Technical Information of China (English)

    王娟; 丁毅; 尹明勇; 马立群

    2012-01-01

    通过植酸活化可提高AZ31镁合金化学镀层的耐蚀性能.采用正交试验优化植酸活化工艺,利用金相显微镜观察了植酸膜的微观形貌,测定了植酸膜在质量分数为3.5%的NaCl溶液中的极化曲线及在化学镀镍液中的开路电位.结果表明,当植酸质量浓度为20 g/L、温度为50 ℃、pH=8时处理25 min,植酸膜具有良好的耐蚀性能,并且能够作为化学镀的活化层.%The corrosion resistance of AZ31 magnesium alloy can be improved by phytic acid activation. The phytic acid activation process was optimized through orthogonal test. The micro-morphology of phytic acid coating was observed by metallographic microscope, and its polarization curve in 3.5wt% NaCl solution and open circuit potential in electroless nickel plating bath were measured. The results showed that the phytic acid coating obtained with phytic acid 20 g/L at 50 ℃ and pH 8 for 25 min has good corrosion resistance and can be used as an activated layer for electroless nickel plating.

  10. Phytic Acid Exposure Alters AflatoxinB1-Induced Reproductive and Oxidative Toxicity in Albino Rats (Rattus norvegicus

    Directory of Open Access Journals (Sweden)

    Abdelaziz S. Abu El-Saad

    2009-01-01

    Full Text Available The increased use of feed in Egypt's aquaculture and animal industries raises concerns about the possible presence of mycotoxins in feedstuffs. The use of alternative medicine, such as botanicals and nutritional supplements, has become popular with inflammatory cases. The present study aimed to testify the role played by phytic acid (IP6 in enhancing the reproductive and oxidative toxicity induced in aflatoxinB1 (AFB1 treated white male albino rats (Rattus norvegicus throughout treatment and withdrawal periods. One hundred and twenty white male albino rats were grouped into four groups. Group 1, was injected with 300 μg kg−1 body wt of AFB1 once every 3 days for 15 days and left uninjected for another 15 days to study the withdrawal effect. Group 2, was injected with 300 μg kg−1 body wt of AFB1 once every 3 days for 15 days and treated simultaneously with IP6 daily for another 15 days. Group 3, was treated daily with IP6 (40 mg kg−1 body wt for 15 days and with no treatment for other 15 days. Group 4, injected with equivalent volume of sterile phosphate buffer saline solution as a control group. Sera were taken at the experimental intervals and assayed for testosterone hormone, follicular-stimulating hormone (FSH and luteinizing hormone (LH to determine the toxicological impact of AFB1 and the possibility of amelioration by phytic acid on the reproductive performance of the studied animal. The effects of AFB1 treatment on the absolute and relative weight of testis as well as its histopathologic effect on the testis and the possibility of amelioration by IP6 treatment were evaluated. The activities of enzymatic and non-enzymatic anti-oxidants, in addition to lipid peroxidation were measured in the testis’ homogenate of AFB1-treated rats. A decrease in sex hormone levels, an increase in testicular lipid peroxidation product levels and a significant decrease in testicular glutathione content, catalase and total peroxidase and superoxide

  11. Adsorption behavior of pH-dependent phytic acid micelles at the copper surface observed by Raman and electrochemistry

    Science.gov (United States)

    Shen, Shu; Du, Juan; Guo, Xiao-yu; Wen, Ying; Yang, Hai-Feng

    2015-02-01

    As heated at 90 °C, phytic acid (PA) molecules in the solution self-organized to form the PA micelles. The size of PA micelles could be tuned by varying pH of the solutions. The adsorption behavior of the different micelles at the copper surface and their corrosion inhibition mechanisms in 0.5 M H2SO4 solution were studied by using electrochemical impedance spectroscopy (EIS), potentiodynamic polarization and surface-enhanced Raman scattering (SERS) spectroscopy. Raman studies showed that the bigger micelles anchoring on the copper surface via P27sbnd O28, P43sbnd O42 and P35sbnd O36 groups, while the smaller PA micelles formed at pH 9 adsorbed at the surface through P35sbnd O36 group. The electrochemical measurements demonstrated that the copper modified with the smaller micelles presented the best inhibition efficiency in 0.5 M H2SO4 solution.

  12. Correlations between the contents of phytic acid and inorganic phosphorous and downy mildew resistance of corn inbred lines

    Directory of Open Access Journals (Sweden)

    Pantipa Na Chiangmai

    2015-10-01

    Full Text Available Seeds of corn inbred lines collected at the National Corn and Sorghum Research Center (NCSRC, Kasetsart University, were analyzed to determine the contents of phytic acid (PA and inorganic phosphorous (InP. These 28 and 29 inbred lines were cultivated at the NCSRC (in the 2008 late rainy season and 2009 early rainy season to evaluate their resistance to corn downy mildew caused by Peronosclerospora sorghi. Results showed that the values of the PA, InP contents and downy mildew infection were statistically different among these inbred lines in both seasons. However, there were no correlations between the contents of either PA or InP and downy mildew infection.

  13. Extraction of phytic acid and preparation of protein isolates from rapeseed meal%菜籽粕植酸提取和分离蛋白的制备

    Institute of Scientific and Technical Information of China (English)

    潘丽军; 周俊; 姜绍通; 孙汉巨; 罗水忠; 韩智宏

    2011-01-01

    植酸和蛋白是菜籽粕中2种极具经济价值的成份.为提高菜籽粕的综合利用效果,该文以双低冷榨菜籽粕为原料,采用醋酸溶液提取植酸,在膜分离精制植酸粗提液过程中同时回收蛋白;再对植酸提取后的残余物进行蛋白分离,超滤纯化后获得高纯度的蛋白成品.响应面优化的植酸最适提取条件为:醋酸质量分数0.7%,提取温度48℃,液料比10 mL/g,提取时间1.6 h,该条件下植酸得率为1.865%.植酸粗提液中回收出的蛋白和损失植酸分别占菜籽粕的3.63%和0.395%.超滤精制的分离蛋白可达到70%~90%不同纯度的要求,蛋白中多酚含量显著减少,且植酸与硫苷未检出.%Phytic acid and protein are two kinds of valuable components in rapeseed meal. To improve comprehensive utilization in this research, phytic acid was extracted with acetum from double-low coldpressed rapeseed meal, and protein was recovered from the crude extract of phytic acid by membrane separating technology. The meal residue was dried to extract protein, which was then purified by ultrafiltration to obtain high purity product. Extraction conditions of phytic acid were optimized by response surface methodology (RSM) as follows: mass fraction of acetic acid 0.7%,extraction temperature 48℃, liquid-to-solid ratio 10 mL/g, extraction time 1.6 h. Under this condition, extraction yield was 1.865%. Protein recovered and phytic acid loss accounting for rapeseed meal were 3.63% and 0.395% respectively in crude extract of phytic acid. Purity of the protein refined by ultrafiltration was between 70% and 90%, in which the content ofpolyphenols was significantly reduced and no phytic acid and glucosinolates could be detected.

  14. Synthesis of a novel electrode material containing phytic acid-polyaniline nanofibers for simultaneous determination of cadmium and lead ions.

    Science.gov (United States)

    Huang, Hui; Zhu, Wencai; Gao, Xiaochun; Liu, Xiuyu; Ma, Houyi

    2016-12-01

    The development of nanostructured conducting polymers based materials for electrochemical applications has attracted intense attention due to their environmental stability, unique reversible redox properties, abundant electron active sites, rapid electron transfer and tunable conductivity. Here, a phytic acid doped polyaniline nanofibers based nanocomposite was synthesized using a simple and green method, the properties of the resulting nanomaterial was characterized by electrochemical impedance spectroscopy (EIS), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS). A glassy carbon electrode modified by the nanocomposite was evaluated as a new platform for the simultaneous detection of trace amounts of Cd(2+) and Pb(2+) using differential pulse anodic stripping voltammetry (DPASV). The synergistic contribution from PANI nanofibers and phytic acid enhances the accumulation efficiency and the charge transfer rate of metal ions during the DPASV analysis. Under the optimal conditions, good linear relationships were obtained for Cd(2+) in a range of 0.05-60 μg L(-1), with the detection limit (S/N = 3) of 0.02 μg L(-1), and for Pb(2+) in a range of 0.1-60 μg L(-1), with the detection limit (S/N = 3) of 0.05 μg L(-1). The new electrode was successfully applied to real water samples for simultaneous detection of Cd(2+) and Pb(2+) with good recovery rates. Therefore, the new electrode material may be a capable candidate for the detection of trace levels of heavy metal ions.

  15. Silencing of ABCC13 transporter in wheat reveals its involvement in grain development, phytic acid accumulation and lateral root formation.

    Science.gov (United States)

    Bhati, Kaushal Kumar; Alok, Anshu; Kumar, Anil; Kaur, Jagdeep; Tiwari, Siddharth; Pandey, Ajay Kumar

    2016-07-01

    Low phytic acid is a trait desired in cereal crops and can be achieved by manipulating the genes involved either in its biosynthesis or its transport in the vacuoles. Previously, we have demonstrated that the wheat TaABCC13 protein is a functional transporter, primarily involved in heavy metal tolerance, and a probable candidate gene to achieve low phytate wheat. In the current study, RNA silencing was used to knockdown the expression of TaABCC13 in order to evaluate its functional importance in wheat. Transgenic plants with significantly reduced TaABCC13 transcripts in either seeds or roots were selected for further studies. Homozygous RNAi lines K1B4 and K4G7 exhibited 34-22% reduction of the phytic acid content in the mature grains (T4 seeds). These transgenic lines were defective for spike development, as characterized by reduced grain filling and numbers of spikelets. The seeds of transgenic wheat had delayed germination, but the viability of the seedlings was unaffected. Interestingly, early emergence of lateral roots was observed in TaABCC13-silenced lines as compared to non-transgenic lines. In addition, these lines also had defects in metal uptake and development of lateral roots in the presence of cadmium stress. Our results suggest roles of TaABCC13 in lateral root initiation and enhanced sensitivity towards heavy metals. Taken together, these data demonstrate that wheat ABCC13 is functionally important for grain development and plays an important role during detoxification of heavy metals.

  16. Performance Evolution of Phytic Acid Conversion Film in the Forming Process

    Directory of Open Access Journals (Sweden)

    Xiufang Cui

    2013-01-01

    Full Text Available To improve conversion film techniques, control film properties and improve quality of following techniques, in this study, the environment-friendly phytic conversion films were deposited on AZ91D magnesium alloy. The performance evolution of the film during the forming process such as mechanical property, residual stress, corrosion resistance, micromorphology, composition, and roughness was investigated by nanomechanical testing system, electrochemical workstation, scanning electron microscope, Auger electron spectroscopy, and atomic force microscope. The results indicate that, in the forming process, the changes of micromorphology, roughness, mechanical property, and corrosion property are closely related to the variation of elements and residual stress. With the prolonging of film formation time, the difference between film elements and substrate elements gradually increases, and the residual stress first increases then decreases. When the film formation time is about 30 min, the film begins to crack and the residual stress is released, which leads to the decrease of mechanical property and corrosion resistance of the film.

  17. Influence of an Escherichia coli-derived phytase on nutrient utilization in broiler starters fed diets containing varying concentrations of phytic acid.

    Science.gov (United States)

    Ravindran, V; Morel, P C H; Partridge, G G; Hruby, M; Sands, J S

    2006-01-01

    The influence of an Escherichia coli-derived phytase, on nutrient utilization was investigated in broilers fed starter diets containing different concentrations of phytate. The study was conducted as a 3 x 4 factorial arrangement of treatments with 3 concentrations of phytic acid (10.4, 11.8, and 13.6 g/kg; equivalent to 2.8, 3.3, and 3.8 g of phytate P/kg) and phytase (0, 500, 750, and 1,000 FTU/kg). One unit of phytase (FTU) is defined as the quantity of enzyme that releases 1 micromol of inorganic phosphorus/min from 0.00015 mol/L of sodium phytate at pH 5.5 at 37 degrees C. The dietary phytic acid concentrations were manipulated by the inclusion of rice bran. Increasing dietary concentrations of phytic acid resulted in reductions (P phytate concentrations. Apparent ileal digestibility coefficients of protein and most amino acids were influenced by phytate (P phytate concentrations, but the trend in responses to increasing phytase additions was different at different phytate concentrations as shown by significant phytate x phytase interactions (P phytate concentration, the ileal digestibility coefficients increased with increasing phytase supplementation. At the medium and high phytate concentrations, the greatest responses were observed at 500 FTU/kg of phytase, with little improvement attributable to further additions. Ileal digestibility of P was lowered (P phytate concentrations and increased (P phytate x phytase interaction (P phytate concentrations. These data demonstrate the anti-nutritive effects of phytic acid and the potential of microbial phytase to improve energy utilization and the availability of P and amino acids in broilers fed starter diets.

  18. Dephytinisation of soyabean protein isolate with low native phytic acid content has limited impact on mineral and trace element absorption in healthy infants.

    Science.gov (United States)

    Davidsson, Lena; Ziegler, Ekhard E; Kastenmayer, Peter; van Dael, Peter; Barclay, Denis

    2004-02-01

    Infant formulas based on soyabean protein isolate are often used as an alternative to cows'-based formulas. However, the presence of phytic acid in soya formulas has raised concern about the absorption of trace elements and minerals from these products. The aim of the present study was to evaluate mineral and trace element absorption from regular and dephytinised soya formula in healthy infants. Soyabean protein isolate with a relatively low native content of phytic acid was used for production of a regular soya formula (300 mg phytic acid/kg liquid formula) and an experimental formula was based on dephytinised soya protein isolate (absorptions were measured by a stable isotope technique based on 72 h faecal excretion of non-absorbed stable isotopes (Zn, Fe, Cu and Ca) and by the chemical balance technique (Mn, Zn, Cu and Ca) in nine infants (69-191 d old). Fe absorption was also measured by erythrocyte incorporation 14 d after intake. The results from the present study demonstrated that Zn absorption, measured by a stable isotope technique, was significantly greater after dephytinisation (mean value 16.7 v. 22.6 %; P=0.03). No other statistically significant differences between the two formulas were observed. The nutritional benefit of dephytinisation was marginal in the present study. Based on these results, the use of soyabean protein isolate with low native content of phytic acid should be promoted for production of soya formulas and adequate addition of ascorbic acid to enhance Fe absorption should be ensured in the products.

  19. The Metabolism and Breeding of Phytic Acid in Maize(Zea mays)%玉米中植酸代谢及其育种

    Institute of Scientific and Technical Information of China (English)

    张少军; 陈茹梅

    2013-01-01

    植酸及其代谢中间体具有重要的生物学功能.禾谷科和油料作物的种子中积累了丰富的植酸.植酸既是一种抗营养因子,也是一种重要的健康因子.然而自植酸被发现至今,人们对于其在植物中的合成过程仍然知之甚少,对其生物学功能更是缺乏全面的了解.近年来,有关于植酸代谢及其功能分析的研究逐渐引起了人们的关注.在玉米(Zea mays)、水稻(Oryza sativa)、大豆(Glycine max)中,人们发展、分离了一系列的低植酸突变体,以期降低种子中的植酸含量,从而获得可用于生产的、能够增强动物磷和矿质营养利用效率并能降低环境污染的新品种.早期的研究发现,植物中植酸的合成途径有两条:依赖于磷脂酰的合成途径和不依赖于磷脂酰的合成途径.有证据表明,作物种子中植酸的积累主要是不依赖于磷脂酰途径的贡献.玉米是中国主要的粮食和饲料作物,关注玉米中植酸代谢和育种的相关研究将有利于动物营养强化以及人类和环境健康.本文综述了植酸的生物学功能以及植酸代谢研究现状,分析并总结了植酸的代谢通路,评述了植酸在玉米中代谢的研究成果,为今后植酸代谢相关的研究提供参考.%Phytic acid and its metabolic intermediates have irreplaceable biological functions. Phytic acid is abundant in cereal grains and soilseeds such as maize and soybean. Phytic acid is an anti-nutritional factor, but it is also an important healthy factor for human. Knowledge about its synthesis and its functions is poorly understood yet, though we know its existence early. For the past few years, a few researches focusing on the metabolism of phytic acid have been reported. Breeders have selected a series of low phytic acid mutant lines in maize (Zea mays), soybean (Glycine max) and rice (Oryza saliva) for future research, which would meet the forage application to enhance the animals' nutrition and to

  20. Digestibility of nutrients in growing-finishing pigs is affected by Aspergillus niger phytase, phytate and lactic acid levels. 2. Apparent total tract digestibility of phosphorus, calcium and magnesium and ileal degradation of phytic acid

    NARCIS (Netherlands)

    Kemme, P.A.; Jongbloed, A.W.; Mroz, Z.; Kogut, J.; Beynen, A.C.

    1999-01-01

    In growing-finishing pigs, the effects of supplemental microbial phytase, lactic acid and Na phytate in a maize-soybean meal based diet on the apparent total tract digestibility (ATTD) of ash, total P, Ca and Mg and the ileal degradation (AID) of phytic acid were studied. The experimental design was

  1. 菜籽饼中植酸的醋酸法提取工艺及其体外抗氧化性研究%Technology of Extracting Phytic Acid with Acetic Acid and Its Antioxidant Activity in Vitro

    Institute of Scientific and Technical Information of China (English)

    汤务霞; 熊治渝; 邓颖强; 吴艳

    2012-01-01

    Rapeseed meal was the main by-products of pressing rapeseed oil and a good resource of phytic acid extraction. Using rapeseed meal as material, technology of extracting phytic acid with acetic acid and its antioxidant activity in vitro were discussed. According to the yield of phytic acid, optimal extraction conditions of phytic acid of repeseed meal by acetic acid was determined by single factor and response surface experiment, selecting extraction temperature, liquid-solid rate, acetic acid mass fraction and extraction time. The result demonstrated that the yield of phytic acid reached 20.22 mg/g on the optimal extraction conditions that extraction temperature was 65℃, liquid-solid rate was 11 : 1, acetic acid mass fraction was 0.75%, and extraction time was 75 min. With phytic acid and vitamin C as a control, by measuring the reduction ability, scavenging capacity of hydroxyl radical and DPpHo of phytic acid extract, the results showed that nhvtic acid extract had certain antinxidant aetlvitv in vitrn%菜籽饼是菜籽压榨制油的副产物,是提取植酸的良好原料。试验以菜籽饼为材料,探索采用醋酸法提取植酸的工艺条件及其体外抗氧化性。以植酸得卒为参考指标,选取提取温度、液料比、醋酸质量分数、提取时间进行单因素和响应面优化试验,得到醋酸法提取菜籽饼中植酸的工艺条件为提取温度65℃、液料比11:1、醋酸质量分数O.75%、提取时N75min。在此工艺条件下植酸得率为20.22mg/g。以标准植酸、维生素C为对照。研究植酸提取液的还原能力及对羟自由基和DPPH-的清除率,试验结果表明,植酸提取液具有一定的体外抗氧化性。

  2. Evaluation of the expression of transcriptional factor NF-kappaB induced by phytic acid in colon cancer cells.

    Science.gov (United States)

    Kapral, Małgorzata; Parfiniewicz, Beata; Strzałka-Mrozik, Barbara; Zachacz, Agnieszka; Weglarz, Ludmiła

    2008-01-01

    Over the last years phytic acid, a hexaphosphorylated inositol (IP6) has attracted particular attention due to its anti-cancer activity, however, the molecular mechanisms of its action have not been elucidated, as yet. The aim of this study was to evaluate the influence of phytic acid on the expression of genes encoding p65 and p50 subunits of NF-kappaB and of its inhibitor IkappaBalpha in human colorectal cancer cell line Caco-2. A kinetic study of p65 and p50 subunits and IkappaBalpha mRNAs expression was performed on Caco-2 cells after treatment with 1, 2.5 and 5 mM IP6 for 1, 6, 12 and 24 h. Quantification of the genes expression was carried out using real time QRT-PCR technique. Treatment of cells with 5 mM IP6 resulted in a strong increase in IkappaBalpha expression at 6 h, 12 h and 24 h. The level of p65 transcript after 1 h was lower in the cells exposed to 1, 2.5, and 5 mM IP6 than in the control cells. The increase in transcriptional activity of p65 gene in response to 5 mM IP6 after 6 h and 12 h was observed. Cells treated for 24 h with 2.5 mM and 5 mM IP6 showed a significant decrease in expression of p65 gene. There were no quantitative changes in the p50 gene expression in the cells treated with IP6 compared to the control cells. High positive correlation between the expression of IkappaBalpha and p65 was detected. The results of this study suggest that IP6 primarily influences p65 and IkappaBalpha genes expression in colon cancer cells. Changes in transcriptional activities of IkappaBalpha and p65 depend on IP6 concentration and time of its action.

  3. Separation and quantitation of phycobiliproteins using phytic acid in capillary electrophoresis with laser-induced fluorescence detection.

    Science.gov (United States)

    Viskari, Pertti J; Colyer, Christa L

    2002-10-01

    The similar electrophoretic mobilities and sizes of several of the phycobiliproteins, which are derived from the photosynthetic apparatus of cyanobacteria and eukaryotic algae, render their separation and quantitation a challenging problem. However, we have developed a suitable capillary electrophoresis (CE) method that employs a phytic acid-boric acid buffer and laser-induced fluorescence (LIF) detection with a single 594 nm He-Ne laser. This method takes advantage of the remarkably high quantum yields of these naturally fluorescent proteins, which can be attributed to their linear tetrapyrrole chromophores covalently bound to cysteinyl residues. As such, limits of detection of 1.18 x 10(-14), 5.26 x 10(-15), and 2.38 x 10(-15) mol/l were obtained for R-phycoerythrin, C-phycocyanin, and allophycocyanin proteins, respectively, with a linear dynamic range of eight orders of magnitude in each case. Unlike previously published CE-LIF methods, this work describes the separation of all three major classes of phycobiliproteins in under 5 min. Very good recoveries, ranging from 93.2 to 105.5%, were obtained for a standard mixture of the phycobiliproteins, based on seven-point calibration curves for both peak height and peak area. It is believed that this development will prove useful for the determination of phycobiliprotein content in naturally occurring cyanobacteria populations, thus providing a useful tool for understanding biological and chemical oceanographic processes.

  4. Removal of aqueous Hg(II) and Cr(VI) using phytic acid doped polyaniline/cellulose acetate composite membrane.

    Science.gov (United States)

    Li, Renjie; Liu, Lifen; Yang, Fenglin

    2014-09-15

    Conductive composite membrane-phytic acid (PA) doped polyaniline (PANI)/cellulose acetate (CA) (PANI-PA/CA) was prepared in a simple and environmental-friendly method, in which aniline was blended with CA/PA solution and polymerized before the phase conversion. The resultant composite membranes were characterized by SEM, EDX, FTIR-ATR, BET and electrical resistance measurements. When used as adsorbent for Hg(II) and Cr(VI) ions, the prepared composite membrane exhibits excellent adsorption capability. The adsorption of Hg(II) and Cr(VI) follows a pseudo-second-order kinetic model and best fits the Langmuir isotherm model, with the maximum adsorption capacity reaching 280.11 and 94.34 mg g(-1), respectively. The heavy metal loaded composite membrane can be regenerated and reused after treatment with acid or alkali solution, making it a promising and practical adsorbent for Hg(II) and Cr(VI) removal. Tests with river water were also carried out, indicating good performance and application.

  5. Enteric-coated tablet of risedronate sodium in combination with phytic acid, a natural chelating agent, for improved oral bioavailability.

    Science.gov (United States)

    Kim, Jeong S; Jang, Sun W; Son, Miwon; Kim, Byoung M; Kang, Myung J

    2016-01-20

    The oral bioavailability (BA) of risedronate sodium (RS), an antiresorptive agent, is less than 1% due to its low membrane permeability as well as the formation of non-absorbable complexes with multivalent cations such as calcium ion (Ca(2+)) in the gastrointestinal tract. In the present study, to increase oral BA of the bisphosphonate, a novel enteric-coated tablet (ECT) dosage form of RS in combination with phytic acid (IP6), a natural chelating agent recognized as safe, was formulated. The chelating behavior of IP6 against Ca(2+), including a stability constant for complex formulation was characterized using the continuous variation method. Subsequently, in vitro dissolution profile and in vivo pharmacokinetic profile of the novel ECT were evaluated comparatively with that of the marketed product (Altevia, Sanofi, US), an ECT containing ethylenediaminetetraacetic acid (EDTA) as a chelating agent, in beagle dogs. The logarithm of stability constant for Ca(2+)-IP6 complex, an equilibrium constant approximating the strength of the interaction between two chemicals to form complex, was 19.05, which was 3.9-fold (pIP6-containing ECT were approximately 7.9- (pIP6 for an oral therapy with the bisphosphonate for improved BA. Copyright © 2015 Elsevier B.V. All rights reserved.

  6. Assessment of the effect of phytic acid on the labeling of blood cells and plasma proteins with Technetium-99m

    Energy Technology Data Exchange (ETDEWEB)

    Lima-Filho, Guilherme L.; Freitas, Rosimeire S.; Moreno, Silvana R.F.; Boasquevisque, Edson M.; Bernardo-Filho, Mario [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Inst. de Biologia. Dept. de Biofisica e Biometria]. E-mail: gllf@hotmail.com; Lima, Glaydes M.T. [Pernambuco Univ., Recife, PE (Brazil). Hospital das Clinicas; Catanho, Maria T.J.A. [Pernambuco Univ., Recife, PE (Brazil). Dept. de Biofisica e Radiobiologia

    2002-07-01

    Blood elements labeled with technetium-99m ({sup 99m} Tc) have been used in various procedures in nuclear medicine. We have investigated if phytic acid (PHY) could alter the labeling of blood elements with {sup 99m} Tc. Blood was incubated with different concentrations of PHY. Stannous chloride and {sup 99m}Tc, as sodium pertechnetate, were added. Blood was centrifuged and plasma (P) and blood cell (BC) were isolated. Samples of P and BC were also precipitated with trichloroacetic acid and centrifuged, and insoluble (IF) and soluble (SF) fractions were separated. The percentages of radioactivity (%ATI) in BC, IF-P and IF-BC were calculated. The %ATI decreased significantly (p < 0.05) in BC (95.08 {+-}1.94 to 80.68 {+-} 3.35), in IF-P (74.42 {+-}4.50 to 39.94{+-} 5.51) and in IF-BC (89.91{+-} 3.91 to 79.54 {+-} 5.42) in presence of PHY. These results suggest that the chelating property of PHY can modify the labeling of the BC, although other effects of PHY could be responsible. As PHY is found in many food and it could alter the labeling of blood elements with {sup 99m} Tc with possible undesirable effects, it is relevant to verify the necessity to repeat the examination and to evaluate the increase of the radiation dose to the patient. (author)

  7. Effect of phytic acid etchant on resin-dentin bonding: Monomer penetration and stability of dentin collagen.

    Science.gov (United States)

    Kong, Kalyan; Hiraishi, Noriko; Nassar, Mohannad; Otsuki, Masayuki; Yiu, Cynthia K Y; Tagami, Junji

    2017-07-01

    Phytic acid (IP6) works well as an etchant in dentin bonding to remove the smear layer due to its acidity and chelating effect. This study compared the etching effect of IP6 with phosphoric acid (PA) and ethylenediaminetetraacetic acid (EDTA) on resin-dentin bond strength, micromorphology of the etched dentin surface and nanoleakage formation along resin-dentin interfaces and compared the protecting effect against collagen degradation. Dentin disks and flat dentin surfaces were obtained from extracted human teeth. Specimens were etched with 35% PA (15s), 0.5M EDTA (30s) or 1% IP6 (30s). The surfaces and longitudinal sections of the etched dentin disks were observed using field emission scanning electron microscope (FE-SEM). An etch-and-rinse adhesive was used to create composite build up-specimens for microtensile bond strength (μTBS) testing and nanoleakage observation. To evaluate the effect on collagen degradation, demineralized bovine root dentin blocks were challenged with bacterial collagenase and then observed under light microscope. PA- and EDTA- treated groups showed significantly lower μTBS when compared to IP6-treated group. PA showed distinct nanoleakage and severe collagen degradation. Only slight nanoleakage was detected in IP6 group. IP6 showed better effect than EDTA in preventing collagen degradation induced by bacterial collagenase. IP6 effectively removed the smear layer and etched dentin, providing high bond strength values and causing minimal nanoleakage and slight collagen degradation. Copyright © 2016 Japan Prosthodontic Society. Published by Elsevier Ltd. All rights reserved.

  8. Polyacrylamide-Phytic Acid-Polydopamine Conducting Porous Hydrogel for Efficient Removal of Water-Soluble Dyes.

    Science.gov (United States)

    Zhao, Zhen; Li, Leijiao; Geleta, Girma Selale; Ma, Lina; Wang, Zhenxin

    2017-08-11

    Removal of toxic dyes from wastewater has become a hot topic in both academic and industrial fields since there is growing concern about the threat of sewage to human health. Herein, we demonstrate that the three-dimensional porous polyacrylamide-phytic acid-polydopamine (termed as PAAM/PA/PDA) hydrogel can be served as reusable adsorbent with high efficiency for either anionic or cationic dyes. Using methyl blue (MB), methylene blue (YMB), methyl violet (MV) and neutral red (NR) as model dyes, we investigate the effect of pH, temperature, dye concentration, and PAAM/PA/PDA hydrogel mass on the adsorption. The experimental maximum adsorption capacities are more than 350.67 mg g(-1) for four selected dyes. Adsorption kinetic and thermodynamic analysis suggests that the dyes are adsorbed on the PAAM/PA/PDA hydrogel through the strong π-π stacking and anion-cation interaction, and the adsorption process satisfies a pseudo-second-order model. Furthermore, the free-standing PAAM/PA/PDA hydrogel can be easily removed from water after adsorption process, and regenerated by adjusting solution pH values.

  9. Phytic acid enhances biocontrol activity of Rhodotorula mucilaginosa against Penicillium expansum contamination and patulin production in apples

    Directory of Open Access Journals (Sweden)

    Qiya eYang

    2015-11-01

    Full Text Available The effect of Rhodotorula mucilaginosa in combination with phytic acid (PA on blue mold decay and patulin contamination of apples was investigated. Results from this study show that different concentrations of PA were effective in reducing the disease incidence of apples and that PA at concentration of 4 μmol/mL, decreased the incidence of blue mold decay in apples from 86.1% to 62.5%, and showed higher control efficacy compared to untreated, control fruit during storage at 20 °C. However, R. mucilaginosa combined with PA (4 μmol/mL showed better control efficacy of blue mold decay than R. mucilaginosa used as single treatment, the disease incidence was reduced to 62.5% and lesion diameter on apples was reduced to 16.59cm. In in vitro experiments, the addition of PA enhanced the biocontrol effect of R. mucilaginosa against the growth of P. expansum and reduced patulin level when compared with either R. mucilaginosa or PA used separately. R. mucilaginosa together with PA, improved the inhibition of patulin production in wounded apples, decreasing the content of patulin by 89.6% compared to the control, under experimental conditions. Both R. mucilaginosa and R. mucilaginosa in combination with PA degraded patulin in vitro. In conclusion, the appropriate combination of R. mucilaginosa and PA may provide an effective biocontrol method for reducing postharvest decay of apples.

  10. The effect of phytic acid on tight junctions in the human intestinal Caco-2 cell line and its mechanism.

    Science.gov (United States)

    Fu, Qingxue; Wang, Huizhen; Xia, Mengxin; Deng, Bing; Shen, Hongyi; Ji, Guang; Li, Guowen; Xie, Yan

    2015-12-01

    This study investigated the effect of phytic acid (IP6), a potential absorption enhancer of flavonoid components, on tight junction (TJ) integrity in Caco-2 cell monolayers and its possible mechanisms. Transepithelial electrical resistance (TEER) across the monolayers decreased rapidly, and the flux of fluorescein sodium (a paracellular marker) increased after treating with IP6 in a concentration-dependent manner. Confocal microscopy results showed that IP6 produced a concentration-dependent attenuation in the distribution of occludin, ZO-1, and claudin-1. Immunoblot analysis revealed that IP6 could down-regulate the expression level of these TJ proteins, which resulted in the opening of TJ. Additionally, the divalent cations Ca(2+) and Mg(2+) influenced the IP6-induced distribution of occludin, ZO-1, and claudin-1 in different directions, which enhanced barrier function. In conclusion, IP6 can decrease the integrity of Caco-2 cell monolayers by modulating the TJ proteins' localization and down-regulating the expression levels of TJ proteins including claudin-1, occludin, and ZO-1; the reduction effects of divalent cations such as Ca(2+) and Mg(2+) on the regulation of TJ induced by IP6 should be addressed. The present work will offer some useful guidance for the application of IP6 in drug delivery area. Copyright © 2015 Elsevier B.V. All rights reserved.

  11. Neuroprotective effect of the natural iron chelator, phytic acid in a cell culture model of Parkinson's disease.

    Science.gov (United States)

    Xu, Qi; Kanthasamy, Anumantha G; Reddy, Manju B

    2008-03-12

    Disrupted iron metabolism and excess iron accumulation has been reported in the brains of Parkinson's disease (PD) patients. Because excessive iron can induce oxidative stress subsequently causing degradation of nigral dopaminergic neurons in PD, we determined the protective effect of a naturally occurring iron chelator, phytic acid (IP6), on 1-methyl-4-phenylpyridinium (MPP(+))-induced cell death in immortalized rat mesencephalic/dopaminergic cells. Cell death was induced with MPP(+) in normal and iron-excess conditions and cytotoxicity was measured by thiazolyl blue tetrazolium bromide (MTT assay) and trypan blue staining. Apoptotic cell death was also measured with caspase-3 activity, DNA fragmentation, and Hoechst nuclear staining. Compared to MPP(+) treatment, IP6 (30 micromol/L) increased cell viability by 19% (PIP6. Cell survival was increased by 18% (PIP6, respectively in iron-excess conditions. A 40% and 52% (PIP6, respectively in iron-excess condition. Similarly, a 45% reduction (PIP6. In addition, Hoechst nuclear staining results confirmed the protective effect of IP6 against apoptosis. Similar protection was also observed with the differentiated cells. Collectively, our results demonstrate a significant neuroprotective effect of phytate in a cell culture model of PD.

  12. Soaking and extrusion effects on physicochemical parameters, phytic acid, nutrient content and mineral bio-accessibility of whole rice grain.

    Science.gov (United States)

    Albarracín, Micaela; José González, Rolando; Drago, Silvina Rosa

    2015-03-01

    A combination of soaking and extrusion processes of whole rice grain was studied. The effects of temperature (35-55 °C) and time (24-48 h) of soaking treatment on phytic acid (PA), protein and ashes losses using a factorial design were evaluated. Taking into account ash, protein and PA losses, whole rice was soaked 24 h at 45 °C and extruded using a Brabender single screw extruder. Effects of extrusion temperature (160-190 °C) and moisture content (14-19 g/100 g) on product characteristics were evaluated using surface response methodology. Values corresponding to the different responses were: Expansion (1.64-3.28), Specific Volume (5.68-11.06 cm(3)/g), Water absorption (3.41-4.43 mL/g) and Solubility (45.44-66.20 g/100 g). The content of PA was reduced from 740.09 to 163.47 mg/100 g (77%) after both processes, resulting in a higher mineral bio-accessibility, and a 7.3% decrease of protein digestibility. Total soluble phenolics and trolox equivalent antioxidant capacity (TEAC) were affected according to the treatment. Both treatments were important to obtain a nutritionally improved whole grain product.

  13. Positional variation in grain mineral nutrients within a rice panicle and its relation to phytic acid concentration.

    Science.gov (United States)

    Su, Da; Sultan, Faisal; Zhao, Ning-chun; Lei, Bing-ting; Wang, Fu-biao; Pan, Gang; Cheng, Fang-min

    2014-11-01

    Six japonica rice genotypes, differing in panicle type, grain density, and phytic acid (PA) content, were applied to investigate the effect of grain position on the concentrations of major mineral nutrients and its relation to PA content and grain weight within a panicle. Grain position significantly affected the concentrations of the studied minerals in both the vertical and horizontal axes of a rice panicle. Heavy-weight grains, located on primary rachis and top rachis, generally had higher mineral concentrations, but were lower in PA concentration and molar ratios of PA/Zn, compared with the small-weight grains located on secondary rachis and bottom rachis, regardless of rice genotypes. However, on the basis of six rice genotypes, no significant correlations were found among mineral elements, PA, and grain weight. These results suggested that some desired minerals, like Zn and Fe, and their bioavailability, can be enhanced simultaneously by the modification of panicle patterns, and it will be helpful in the selection of rice genotypes with low PA and high mineral nutrients for further breeding strategy without sacrificing their high yields.

  14. Laccase biosensor based on phytic acid modification of nanostructured SiO₂ surface for sensitive detection of dopamine.

    Science.gov (United States)

    Zhao, Wenbo; Wang, Kuai; Wei, Yuan; Ma, Yinghui; Liu, Lingling; Huang, Xiaohua

    2014-09-23

    In this work, three kinds of nanostructured silica-phytic acid (SiO2-PA) materials with diverse morphologies including spherical SiO2-PA (s-SiO2-PA), rod-like (r-SiO2-PA), and helical SiO2-PA (h-SiO2-PA) were prepared with the help of electrostatic interaction. The SiO2-PA nanomaterials with different morphologies were characterized by using transmission electron microscopy (TEM), Fourier transform infrared (FTIR), electrochemical impedance spectroscopy (EIS), and circular dichroism spectrum (CD). Diverse morphologies of SiO2-PA were used as electrode decorated materials to achieve a high efficiency for electrochemical dopamine (DA) detection. The laccase biosensors were fabricated by immobilizing different morphologies of SiO2-PA nanomaterials and laccase onto the glassy carbon electrode (GCE) surface, successively. Then the electrochemical responses of the different morphologies of nanostructured SiO2-PA nanomaterials to laccase were discussed. Results indicated that compared to laccase/s-SiO2-PA and laccase/r-SiO2-PA, the laccase/h-SiO2-PA-modified electrode showed the best electrochemical performances.

  15. Effects of dietary soybean stachyose and phytic acid on gene expressions of serine proteases in Japanese flounder ( Paralichthys olivaceus)

    Science.gov (United States)

    Mi, Haifeng; Mai, Kangsen; Zhang, Wenbing; Wu, Chenglong; Cai, Yinghua

    2011-09-01

    Soybean stachyose (SBS) and phytic acid (PA) are anti-nutritional factors (ANF) which have deleterious effects on the growth and digestibility in fish. The present research studied the effects of dietary SBS and PA on the expression of three serine protease genes in the liver of Japanese flounder ( Paralichthys olivaceus). These genes are trypsinogen 1 (poTRY), elastase 1 (poEL) and chymotrypsinogen 1 (poCTRY). Eight artificial diets with graded levels of supplemented ANFs were formulated to 4 levels of SBS (0.00, 0.40, 0.80 and 1.50%), 4 levels of PA (0.00, 0.20, 0.40 and 0.80), respectively. Japanese flounder (initial weight 2.45 g ± 0.01 g) were fed with these diets for 10 weeks with three replications per treatment. At the end of 10 weeks, supplementation of 0.40% of dietary SBS or PA significantly increased the gene expression of poTRY and poCTRY ( P0.05). These results suggested that dietary SBS and dietary PA could directly affect the serine protease genes at the transcriptional level in Japanese flounder, and these genes' expression was more sensitive to dietary PA than to SBS under the current experimental conditions.

  16. Reduction in phytic acid content and enhancement of antioxidant properties of nutricereals by processing for developing a fermented baby food.

    Science.gov (United States)

    Rasane, Prasad; Jha, Alok; Kumar, Arvind; Sharma, Nitya

    2015-06-01

    Cereal blends containing pearl millet (Pennisetum glaucum), sorghum (Sorghum bicolor) and oat (Avena sativa) in different ratios were processed (roasted and germinated) and also used as unprocessed flours followed by fermentation with Lactobacillus sp. (Lactobacillus casei and Lactobacillus plantarum). They were screened for total phenolic content (TPC), phytic acid content (PAC) and free radical scavenging activity (FRSA). A formulation with the highest TPC, FRSA and the lowest PAC was selected to optimize a nutricereal based fermented baby food containing selected fermented cereal blends (FCB), rice-corn cooked flour (RCF), whole milk powder (WMP), whey protein concentrate (WPC) and sugar. The optimized baby food formulation contained 37.41 g 100 g(-1) FCB, 9.75 g 100 g(-1) RCF, 27.84 g 100 g(-1) WMP, 5 g 100 g(-1) WPC and 20 g 100 g(-1) sugar. It had high protein, vitamin, minerals, as well as good quantity of carbohydrates and fat, to fulfil the nutritional needs of preschool children of age 1-3 years. The nutricereal based fermented baby food showed high water absorption capacity, dispersibility, wettability and flowability indicating good reconstitution properties.

  17. Phytic Acid Enhances Biocontrol Activity of Rhodotorula mucilaginosa against Penicillium expansum Contamination and Patulin Production in Apples.

    Science.gov (United States)

    Yang, Qiya; Zhang, Hongyin; Zhang, Xiaoyun; Zheng, Xiangfeng; Qian, Jingya

    2015-01-01

    The effect of Rhodotorula mucilaginosa in combination with phytic acid (PA) on blue mold decay and patulin contamination of apples was investigated. Results from this study show that different concentrations of PA were effective in reducing the disease incidence of apples and that PA at concentration of 4 μmol/mL, decreased the incidence of blue mold decay in apples from 86.1 to 62.5%, and showed higher control efficacy compared to untreated, control fruit during storage at 20°C. However, R. mucilaginosa combined with PA (4 μmol/mL) showed better control efficacy of blue mold decay than R. mucilaginosa used as single treatment, the disease incidence was reduced to 62.5% and lesion diameter on apples was reduced to 16.59 cm. In in vitro experiments, the addition of PA enhanced the biocontrol effect of R. mucilaginosa against the growth of Penicillium expansum and reduced patulin level when compared with either R. mucilaginosa or PA used separately. R. mucilaginosa together with PA, improved the inhibition of patulin production in wounded apples, decreasing the content of patulin by 89.6% compared to the control, under experimental conditions. Both R. mucilaginosa and R. mucilaginosa in combination with PA degraded patulin in vitro. In conclusion, the appropriate combination of R. mucilaginosa and PA may provide an effective biocontrol method for reducing postharvest decay of apples.

  18. 菜籽预榨-浸出粕中植酸的提取%Extraction of phytic acid from prepress - solvent extracted rapeseed meal

    Institute of Scientific and Technical Information of China (English)

    鲍传稳; 张明辉; 刘晔

    2012-01-01

    为实现菜籽粕的综合开发利用,研究了一种从菜籽预榨-浸出粕中两步提取植酸的工艺.考察了酸洗pH、酸洗时间、碱提pH和碱提乙二胺四乙酸二钠(EDTA)浓度等对植酸提取的影响.将经过复合纤维素酶水解的菜籽粕在pH 5.0和50℃下酸洗3h获得酸洗液,然后用EDTA浓度0.1mol/L的碱性溶液在pH 12.0和50℃下碱提酸沉获得乳清液,经以上两步可将菜籽粕中的植酸基本提取完全.将提取液中的粗植酸经氢氧化钙沉淀并酸化,再经离子交换可获得纯度为69.96%、提取率为58.0%的植酸产品.%To realize fully utilization of rapeseed meal, a two - step process for extraction of phytic acid from prepress - solvent extracted rapeseed meal was established. The effects of key parameters on phytic acid extraction, including pH of acid solution, duration of acid solution, pH and ethylene diamine tetraace-tic acid(EDTA) concentration of alkaline solution for extraction were investigated. With an acid solution extracting step at pH 5.0 and 501 for 3 h and an alkaline solution extracting step at pH 12.0 and 50℃ with the supplement of 0.1 mol/L EDTA followed by acidic precipitation, the phytic acid in cellulase pre-treated rapeseed meal could be almost entirely extracted. The crude phytic acid in extracted solution was purified by calcium hydroxide precipitation and acidification, then it was treated with ion exchange to obtain a final product with purity of 69. 96% and extraction rate of 58.0%.

  19. Effects of lactoferrin, phytic acid, and EDTA on oxidation in two food emulsions enriched with long-chain polyunsaturated fatty acids

    DEFF Research Database (Denmark)

    Nielsen, Nina Skall; Petersen, A.; Meyer, Anne S.

    2004-01-01

    The influence of the addition of metal chelators on oxidative stability was studied in a milk drink and in a mayonnaise system containing highly polyunsaturated lipids. Milk drinks containing 5% (w/w) of specific structured lipid were supplemented with lactoferrin (6-24 M) and stored at 2 C for up...... to 9 weeks. Mayonnaise samples with 16% fish oil and 64% rapeseed oil (w/w) were supplemented with either lactoferrin (8-32 M), phytic acid (16-124 M), or EDTA (16-64 M) and were stored at 20 C for up to 4 weeks. The effect of the metal chelators was evaluated by determination of peroxide values......, secondary volatile oxidation products, and sensory analysis. Lactoferrin reduced the oxidation when added in concentrations of 12 M in the milk drink and 8 M in the mayonnaise, whereas it was a prooxidant at higher concentrations in both systems. In mayonnaise, EDTA was an effective metal chelator even...

  20. Seed-specific silencing of OsMRP5 reduces seed phytic acid and weight in rice.

    Science.gov (United States)

    Li, Wen-Xu; Zhao, Hai-Jun; Pang, Wei-Qin; Cui, Hai-Rui; Poirier, Yves; Shu, Qing-Yao

    2014-08-01

    Phytic acid (PA) is poorly digested by humans and monogastric animals and negatively affects human/animal nutrition and the environment. Rice mutants with reduced PA content have been developed but are often associated with reduced seed weight and viability, lacking breeding value. In the present study, a new approach was explored to reduce seed PA while attaining competitive yield. The OsMRP5 gene, of which mutations are known to reduce seed PA as well as seed yield and viability, was down-regulated specifically in rice seeds by using an artificial microRNA driven by the rice seed specific promoter Ole18. Seed PA contents were reduced by 35.8-71.9% in brown rice grains of transgenic plants compared to their respective null plants (non-transgenic plants derived from the same event). No consistent significant differences of plant height or number of tillers per plant were observed, but significantly lower seed weights (up to 17.8% reduction) were detected in all transgenic lines compared to null plants, accompanied by reductions of seed germination and seedling emergence. It was observed that the silencing of the OsMRP5 gene increased the inorganic P (Pi) levels (up to 7.5 times) in amounts more than the reduction of PA-P in brown rice. This indicates a reduction in P content in other cellular compounds, such as lipids and nucleic acids, which may affect overall seed development. Put together, the present study demonstrated that seed specific silencing of OsMRP5 could significantly reduce the PA content and increase Pi levels in seeds; however, it also significantly lowers seed weight in rice. Discussions were made regarding future directions towards producing agronomically competitive and nutritionally valuable low PA rice.

  1. The oxygen isotope composition of phosphate released from phytic acid by the activity of wheat and Aspergillus niger phytase

    Science.gov (United States)

    von Sperber, C.; Tamburini, F.; Brunner, B.; Bernasconi, S. M.; Frossard, E.

    2015-07-01

    Phosphorus (P) is an essential nutrient for living organisms. Under P-limiting conditions plants and microorganisms can exude extracellular phosphatases that release inorganic phosphate (Pi) from organic phosphorus compounds (Porg). Phytic acid (myo-inositol hexakisphosphate, IP6) is an important form of Porg in many soils. The enzymatic hydrolysis of IP6 by phytase yields available Pi and less phosphorylated inositol derivates as products. The hydrolysis of organic P compounds by phosphatases leaves an isotopic imprint on the oxygen isotope composition (δ18O) of released Pi, which might be used to trace P in the environment. This study aims at determining the effect of phytase on the oxygen isotope composition of released Pi. For this purpose, enzymatic assays with histidine acid phytases from wheat and Aspergillus niger were prepared using IP6, adenosine 5'-monophosphate (AMP) and glycerophosphate (GPO4) as substrates. For a comparison to the δ18O of Pi released by other extracellular enzymes, enzymatic assays with acid phosphatases from potato and wheat germ with IP6 as a substrate were prepared. During the hydrolysis of IP6 by phytase, four of the six Pi were released, and one oxygen atom from water was incorporated into each Pi. This incorporation of oxygen from water into Pi was subject to an apparent inverse isotopic fractionation (ϵ ~ 6 to 10 ‰), which was similar to that imparted by acid phosphatase from potato during the hydrolysis of IP6 (ϵ ~ 7 ‰), where less than three Pi were released. The incorporation of oxygen from water into Pi during the hydrolysis of AMP and GPO4 by phytase yielded a normal isotopic fractionation (ϵ ~ -12 ‰), similar to values reported for acid phosphatases from potato and wheat germ. We attribute this similarity in ϵ to the same amino acid sequence motif (RHGXRXP) at the active site of these enzymes, which leads to similar reaction mechanisms. We suggest that the striking

  2. The oxygen isotope composition of phosphate released from phytic acid by the activity of wheat and Aspergillus niger phytase

    Directory of Open Access Journals (Sweden)

    C. v. Sperber

    2015-03-01

    Full Text Available Phosphorus (P is an essential nutrient for living organisms. Under P-limiting conditions plants and microorganisms can exude extracellular phosphatases that release inorganic phosphate (Pi from organic phosphorus compounds (Porg. Phytic acid (IP6 is an important form of Porg in many soils. The enzymatic hydrolysis of IP6 by phytase yields plant available inorganic phosphate (Pi and less phosphorylated inositol derivates as products. The hydrolysis of organic P-compounds by phosphatases leaves an isotopic imprint on the oxygen isotope composition (δ18O of released Pi, which might be used to trace P in the environment. This study aims at determining the effect of phytase on the oxygen isotope composition of released Pi. For this purpose, enzymatic assays with histidine acid phytases from wheat and Aspergillus niger were prepared using IP6, adenosine 5'monophosphate (AMP and glycerophosphate (GPO4 as substrates. For a comparison to the δ18O of Pi released by other extracellular enzymes, enzymatic assays with acid phosphatases from potato and wheat germ with IP6 as substrate were prepared. During the hydrolysis of IP6 by phytase, four Pi are released, and one oxygen atom from water is incorporated into each Pi. This incorporation of oxygen from water into Pi is subject to an apparent inverse isotopic fractionation (ϵ ∼ 6 to 10‰, which is similar to that imparted by acid phosphatase from potato during the hydrolysis of IP6 (ϵ ∼ 7‰ where less than three Pi are released. The incorporation of oxygen from water into Pi during the hydrolysis of AMP and GPO4 by phytase yielded a normal isotopic fractionation (ϵ ∼ −12‰, again similar to values reported for acid phosphatases from potato and wheat germ. We attribute this similarity in ε to the same amino acid sequence motif (RHGXRXP at the active site of these enzymes, which leads to similar reaction mechanisms. We suggest that the striking substrate

  3. Phytic Acid and Sodium Chloride Show Marked Synergistic Bactericidal Effects against Nonadapted and Acid-Adapted Escherichia coli O157:H7 Strains.

    Science.gov (United States)

    Kim, Nam Hee; Rhee, Min Suk

    2015-12-04

    The synergistic antimicrobial effects of phytic acid (PA), a natural extract from rice bran, plus sodium chloride against Escherichia coli O157:H7 were examined. Exposure to NaCl alone at concentrations up to 36% (wt/wt) for 5 min did not reduce bacterial populations. The bactericidal effects of PA alone were much greater than those of other organic acids (acetic, citric, lactic, and malic acids) under the same experimental conditions (P acid-adapted cells, reducing their numbers to unrecoverable levels (>7-log CFU/ml reduction). Flow cytometry confirmed that PA disrupted the cell membrane to a greater extent than did other organic acids, although the cells remained viable. The combination of PA and NaCl induced complete disintegration of the cell membrane. By comparison, none of the other organic acids acted synergistically with NaCl, and neither did NaCl-HCl solutions at the same pH values as the test solutions of PA plus NaCl. These results suggest that PA has great potential as an effective bacterial membrane-permeabilizing agent, and we show that the combination is a promising alternative to conventional chemical disinfectants. These findings provide new insight into the utility of natural compounds as novel antimicrobial agents and increase our understanding of the mechanisms underlying the antibacterial activity of PA.

  4. Excellent anti-corrosive pretreatment layer on iron substrate based on three-dimensional porous phytic acid/silane hybrid

    Science.gov (United States)

    Gao, Xiang; Lu, Ke; Xu, Lei; Xu, Hua; Lu, Haifeng; Gao, Feng; Hou, Shifeng; Ma, Houyi

    2016-01-01

    A novel, highly effective and environmentally friendly film-forming material, phytic acid (PA)/silane (denoted as PAS) hybrid with a three-dimensional (3D) network structure, was prepared through a condensation reaction of PA with methyltrihydroxysilane generated from the hydrolysis of methyltriethoxysilane (MTES). Two kinds of PAS-based pretreatment layers, namely NaBrO3-free and NaBrO3-doped PAS layers, were fabricated on iron substrates using the dip-coating method. SEM and AFM observations showed that the as-fabricated PAS-based layers possessed a 3D porous microstructure at the nanoscale and a rough surface morphology. X-ray photoelectron spectroscopic (XPS) and attenuated total reflection infrared (ATR-IR) spectroscopic characterization demonstrated that the above PAS layers bound to the iron surface via the -P-O- bond. Moreover, analyses of steady-state polarization curves and electrochemical impedance spectroscopic (EIS) data indicated that the corrosion rates of the iron substrates decreased considerably in the presence of the two PAS-based pretreatment layers. In particular, the NaBrO3-dosed PAS layer displayed the better corrosion resistance ability as well as maintaining the original microstructure and surface morphology. The PAS-based pretreatment layers are expected to act as substitutes for chromate and phosphate conversion layers and will find widespread application in the surface pretreatment of iron and steel materials due to the advantages of being environmentally friendly, the rapid film-forming process, and, especially, the nanoporous microstructure and rough surface morphology.A novel, highly effective and environmentally friendly film-forming material, phytic acid (PA)/silane (denoted as PAS) hybrid with a three-dimensional (3D) network structure, was prepared through a condensation reaction of PA with methyltrihydroxysilane generated from the hydrolysis of methyltriethoxysilane (MTES). Two kinds of PAS-based pretreatment layers, namely Na

  5. Histomorphology and small intestinal sodium-dependent glucose transporter 1 gene expression in piglets fed phytic acid and phytase-supplemented diets.

    Science.gov (United States)

    Woyengo, T A; Rodriguez-Lecompte, J C; Adeola, O; Nyachoti, C M

    2011-08-01

    An experiment was conducted to determine the effect of dietary phytic acid (PA) and phytase supplementation on small intestinal histomorphology and Na-dependent glucose transporter 1 (SGLT1) gene expression in piglets. Twenty-four piglets with an average initial BW of 7.60 ± 0.73 kg were randomly assigned to 3 experimental diets, to give 8 piglets per diet. The diets were a casein-cornstarch-based diet that was supplemented with 0 or 2% PA, or 2% PA (as Na phytate) plus an Escherichia coli-derived phytase at 500 phytase units/kg. The basal diet was formulated to meet the 1998 NRC energy, digestible AA, mineral, and vitamin requirements for piglets. After 10 d of feeding, the piglets were killed to determine small intestinal histomorphology and small intestinal SGLT1 gene expression. Phytic acid supplementation did not affect (P > 0.1) villus height (VH) and the VH-to-crypt depth (CD) ratio, but did decrease (P 0.1) VH, CD, and the VH-to-CD ratio. Phytic acid supplementation reduced SGLT1 gene expression in the duodenum, jejunum, and ileum by 1.1-, 5.4-, and 2.4-fold, respectively. Phytase supplementation increased SGLT1 gene expression in the jejunum by 2.6-fold, but reduced SGLT1 gene expression in the duodenum and ileum by 2.0- and 4.0-fold, respectively. In conclusion, PA reduced CD in the jejunum and SGLT1 gene expression in the duodenum, jejunum, and ileum, whereas phytase supplementation increased the expression of SGLT1 in the jejunum. The reduced SGLT1 gene expression by PA implies that PA reduces nutrient utilization in pigs partly through reduced expression of SGLT1, which is involved in glucose and Na absorption. The increased expression of SGLT1 in the jejunum by phytase supplementation implies that phytase alleviated the negative effects of PA partly through increased expression of SGLT1.

  6. Polyacrylamide-phytic acid-polydopamine conducting porous hydrogel for rapid detection and removal of copper (II) ions.

    Science.gov (United States)

    Zhao, Zhen; Chen, Hongda; Zhang, Hua; Ma, Lina; Wang, Zhenxin

    2017-05-15

    In this work, a conducting porous polymer hydrogel-based electrochemical sensor has been developed for rapid detection of copper (II) ions (Cu(2+)). The polymer (termed as PAAM/PA/PDA) hydrogel is prepared through multi-interactions of the monomers dopamine (DA), acrylamide (AAM) and phytic acid (PA) under mild ambient conditions: the AAM polymerizes through free-radical polymerization, DA occurs poly coupling reaction, and PA crosslinks polydopamine (PDA) and polyacrylamide (PAAM) by hydrogen bonds. The three dimensional (3D) network nanostructured PAAM/PA/PDA hydrogel not only provides a large surface area for increasing the amount of immobilized molecules/ions, but also exhibits a good conductivity. The PAAM/PA/PDA hydrogel-based electrochemical sensor exhibits a low detection limit (1nmolL(-)(1), S/N=3) and wide linear range (from 1nmolL(-)(1) to 1µmolL(-1)) for Cu(2+) detection in aqueous samples. Furthermore, the Cu(2+) can be sensitively detected by the electrochemical sensor in different sample matrices, indicating that the electrochemical sensor could be used to monitor Cu(2+) with reasonable assay performance in practical samples. The PAAM/PA/PDA hydrogel also exhibits a good capacity to remove Cu(2+)(231.36±4.70mgg(-1)), which is superior to those of other adsorption materials reported in the literature. The facile synthesized PAAM/PA/PDA hydrogel provides a novel and regenerable platform for monitoring and removing Cu(2+) in real samples. Copyright © 2016 Elsevier B.V. All rights reserved.

  7. Effects of Dietary Soybean Stachyose and Phytic Acid on Gene Expressions of Serine Proteases in Japanese Flounder (Paralichthys olivaceus)

    Institute of Scientific and Technical Information of China (English)

    MI Haifeng; MAI Kangsen; ZHANG Wenbing; WU Chenglong; CAI Yinghua

    2011-01-01

    Soybean stachyose (SBS) and phytic acid (PA) are anti-nutritional factors (ANF) which have deleterious effects on the growth and digestibility in fish.The present research studied the effects of dietary SBS and PA on the expression of three serine protease genes in the liver of Japanese flounder (Paralichthys olivaceus).These genes are trypsinogen 1 (poTRY),elastase 1 (poEL) and chymotrypsinogen 1 (poCTRY).Eight artificial diets with graded levels of supplemented ANFs were formulated to 4 levels of SBS (0.00,0.40,0.80 and 1.50%),4 levels of PA (0.00,0.20,0.40 and 0.80),respectively.Japanese flounder (initial weight 2.45 g±0.01 g)were fed with these diets for 10 weeks with three replications per treatment.At the end of 10 weeks,supplementation of 0.40% of dietary SBS or PA significantly increased the gene expression ofpoTRY and poCTRY (P<0.05).The same level of dietary SBS significantly decreased the gene expression of poEL.In comparison with the control group (ANF-free),dietary PA (0.2% and 0.8%)significantly decreased the gene expression ofpoTRY,poCTRY and poEL (P<0.05).However,excessive supplement of dietary SBS (1.5%) has no significant effects on these gene expressions (P>0.05).These results suggested that dietary SBS and dietary PA could directly affect the serine protease genes at the transcriptional level in Japanese flounder,and these genes' expression was more sensitive to dietary PA than to SBS under the current experimental conditions.

  8. Effect of phytic acid and inositol on the proliferation and apoptosis of cells derived from colorectal carcinoma.

    Science.gov (United States)

    Schröterová, L; Hasková, P; Rudolf, E; Cervinka, M

    2010-03-01

    We characterized the effect of phytic acid (inositol hexaphosphate, IP6) as a potential adjuvant in treatment of colorectal carcinoma and evaluated the optimal concentration and treatment time to produe the maximal therapeutic effect. There is some evidence that myoinositol (Ins) can potentiate anti-cancer effects of IP6. Therefore, we tested both IP6 and Ins individually and in combination on human cell lines HT-29, SW-480 and SW-620 derived from colorectal carcinoma in different stages of malignancy. The effect of tested chemicals on the cells was measured using metabolic activity assay (WST-1), DNA synthesis assay (BrdU), protein synthesis assay (Brilliant Blue) and apoptosis (caspase-3 activity). We tested IP6 and Ins at three concentrations: 0.2, 1 and 5 mM for 24, 48 and 72 h. The concentrations and incubation periods were chosen according to low toxicity of the tested substance that was observed in a long-term clinical study. We found that all employed concentrations of IP6 or IP6/Ins decreased proliferation of the cell lines, with the maximum decrease being observed in HT-29 cells. Metabolic activity of treated cells differed in response to IP6 and IP6/Ins treatment; in HT-29 and SW-620 significant decrease was observed only at the highest concentration, whereas in SW-480 cells metabolic activity was lower at each concentration except 0.2 and 1 mM IP6 or IP6/Ins in 24-h incubation. The results from protein content assay corresponded to the results obtained from WST assay. In addition, we found maximum increase in caspase-3 activity at concentration 5 mM IP6 or IP6/Ins in HT-29 cells and with IP6 at concentration of 0.2 mM or IP6/Ins in SW-480 cells with clear indication of Ins enhancing the proapoptotic effect of IP6 in all the cell lines studied.

  9. The effect of balanced fertilization on nutrients’ concentration and phytic acid to zinc molar ratio in Iranian red been (Phaseolus calcaratus L. cultivars at different stages of seed development

    Directory of Open Access Journals (Sweden)

    B. Motesharezadeh

    2012-06-01

    Full Text Available Phytic acid is the main source of organic phosphorus in grains of legumes and cereals. It has great ability of bonding with metals and minerals such as iron, zinc and calcium. As a result, the solubility of these elements and their absorption capability by human will be reduced. This greenhouse research was carried out with the aim of studying the variations of phytic acid to zinc molar ratio and evaluation of nutrients’ concentration in different varieties of red bean (Phaseolus calcaratus L. under balanced fertilization conditions (based on soil test in different timings after flowering stage. Treatments included: 5 varieties of red bean (Akhtar, Naz, Derakhshan, Goli and Sayyad, two levels of fertilizer (control and balanced fertilization and 3 times of sampling (12, 22 and 32 days after flowering; T1, T2 and T3, respectively in a factorial arrangement with complete randomized blocks design. Phosphorous, potassium, iron and zinc concentration and phytic acid to zinc molar ratio in grains of different red bean cultivars were determined. The results of analysis of variance showed that the effect of cultivar, fertilizer and sampling time on concentration of zinc, iron, potassium, phosphorous and phytic acid to zinc molar ratio in grain was significant (P<0.01. Also, the highest concentration of zinc in grain was measured in Goli cultivar at T3 timing. The phytic acid to zinc molar ratio in balanced-fertilization treatment was recorded for Goli cultivar at T1, T2 and T3 timings as 11.1, 10.49 and 7.99, respectively. In general, the results of this research showed that balanced fertilization reduced phytic acid to zinc molar ratio. This can be effective in enhancing humans’ health by improving the absorption capability of required nutrients. More investigations under field conditions are recommended.

  10. Kunitz trypsin Inhibitor and phytic acid levels in conventional and genetically modified soybean seeds from Londrina and Ponta Grossa, South Brazil.

    OpenAIRE

    Olívio F. Galão; Carrão-Panizzi,Mercedes C; Mandarino,José Marcos G.; Rodrigo Santos Leite; Thiago Claus; Jesuí Vergílio Visentainer

    2014-01-01

    Differences in the levels of antinutritional components - Kunitz trypsin inhibitor and phytic acid - were determined for conventional and genetically modified soybean cultivated in Londrina and Ponta Grossa, South Brazil. Trypsin inhibitor levels in the conventional cultivars of Londrina and Ponta Grossa varied from 14.56 mg g-1 (BRS 267) to 20.40 mg g-1 (BRS 261) and from 13.51 mg g-1 (BRS 232) to 19.65 mg g-1 (BRS 268), respectively, whereas in the genetically modified (GM) cultivars, they ...

  11. Iron, zinc and phytic acid in rice from China: wet and dry processing towards improved mineral bioavailability

    NARCIS (Netherlands)

    Liang, J.

    2007-01-01

    Rice and rice products supply two thirds of Chinese people with their staple food. Mineral deficiencies, especially of iron and zinc, are prevalent in China, and are caused by insufficient intake and poor bioavailability. Rice and rice products contribute more than 50% of the antinutrient  phytic ac

  12. Enhanced corrosion resistance of phytic acid coated magnesium by stearic acid treatment%硬脂酸处理提高植酸包覆镁的耐腐蚀性能

    Institute of Scientific and Technical Information of China (English)

    R.K.GUPTA; K.MENSAH-DARKWA; J.SANKAR; D.KUMAR

    2013-01-01

    将镁浸泡在植酸溶液中,在其表面形成一层化学转化膜.然后再将其浸泡在硬脂酸溶液中以改善植酸转化膜的显微组织和抗腐蚀性能.经过硬脂酸溶液浸泡处理后,转化膜未出现裂纹,试样表面变得光滑.采用电化学方法研究其腐蚀行为.结果表明,经硬脂酸溶液浸泡处理的试样比未经浸泡处理的和没有转化膜的试样具有更高的耐腐蚀性能.因此,硬脂酸溶液处理能够增强镁的耐腐蚀性能.%A green chemical conversion coating for magnesium was obtained with a phytic acid solution.The microstructure and corrosion properties of phytic acid conversion coated magnesium were further improved by soaking in stearic acid solution.The phytic acid conversion coated magnesium after soaking in the stearic acid showed no micro-cracks and the surface became very smooth.The corrosion behavior of the uncoated and coated magnesium samples was studied by electrochemical methods.The corrosion resistance of the stearic acid treated sample was much higher than that of phytic acid conversion coated magnesium or uncoated magnesium.The electrochemical results indicated that the stearic acid treated coating provided effective corrosion protection to the magnesium sample.

  13. Determination on Degradation Condition of Phytic Acid in Rice Bran Fermented by Bacillus Natto%纳豆芽孢杆菌发酵米糠中植酸降解的测定

    Institute of Scientific and Technical Information of China (English)

    祁红兵; 宋莉; 陈钧

    2009-01-01

    优化了植酸分析样品的前处理工艺.在64 h 发酵过程中,米糠发酵物中植酸含量随发酵时间的延长而下降,降解率为34%.%The pretreatment process for analysis sample of phytic acid was optimized. In the whole fermentation process for 64 h,the phytic acid content in fermentation broth of rice bran was decreased with the delay of fermentation time,and its degradation rate was 34%.

  14. Optimization of Extraction Condition for Phytic Acid from Mulberry Leaf and Determination of Phytic Acid Contents in Mulberry Leaf and Silkworm Excrement%桑叶植酸的提取条件优化及桑叶和蚕粪中的植酸含量检测

    Institute of Scientific and Technical Information of China (English)

    李镇刚; 赵爱春; 王茜龄; 吴文铂; 金筱耘; 李军; 余茂德

    2012-01-01

    建立高效提取桑叶植酸的方法,并应用于检测分析不同品种、不同成熟度桑叶中的植酸含量和蚕粪中的植酸含量,有助于进一步开展降低桑叶中的植酸含量及提高桑叶营养利用率的研究.运用Design-Expert 8.0.4 Trial软件的Box-Behnken模型,分析不同提取条件因子对桑叶植酸提取效果的影响作用为原料质量浓度>提取液中盐酸的浓度>浸提时间,确定最佳提取条件:提取液为1.25%盐酸+0.1 g/mL硫酸钠,浸提时间为2.18h,原料质量浓度为0.05 g/mL.在此优化条件下提取桑叶和蚕粪中的植酸,并应用比色法测定5个桑品种春季成熟叶、嫩叶以及秋季成熟叶、嫩叶中的植酸平均质量比分别为3.98、3.55、3.75、3.52 mg/g,5龄幼虫蚕粪中的植酸含量为3.78 mg/g,与桑叶中的植酸含量接近.检测结果说明桑叶中的植酸随着桑叶的成熟其含量不断增加,家蚕不能吸收利用植酸态的磷.%The establishment of extraction protocol for phytic acid from mulberry leaf and its application of the method for determining phytic acid contents in mulberry leaves of different varieties and different maturities and in silkworm excrement would promote further studies on reducing phytic acid content in mulberry leaves and improving their nutrient utilization rate.In this study,we analyzed effect of different extraction conditions and factors on the yield of phytic acid from mulberry leaves by means of Box-Behnken model of the Design-Expert 8.0.4 Trial software.The results showed that the effective factors for phytic acid extraction were in the order of raw material mass concentration,hydrochloric acid concentration in the extraction solution,and extraction time.The optimum extraction condition was as follows: 1.25% hydrochloric acid and 0.1 g/mL sodium sulfate in extraction solution,extraction time 2.18 h,and raw material mass concentration 0.05 g/mL.Based on this optimum condition,we extracted phytic acid from

  15. Response Surface Methodology Optimize Extraction of Phytic Acid in Rapeseed%响应面法优化菜籽粕中植酸提取工艺研究

    Institute of Scientific and Technical Information of China (English)

    罗仓学; 史兰; 李振尧

    2013-01-01

    利用低浓度(体积分数)的醋酸溶液,在单因素试验的基础上选择了醋酸浓度、提取温度和提取时间进行响应面分析,以提取液中植酸含量(质量分数)为响应值,建立了植酸提取的二次多项式数学模型,确定了植酸提取的最佳工艺条件为:醋酸浓度4.4%,提取温度41℃,提取时间2h,得到提取液中植酸含量为1.64%。%Using low concentration (volume fraction) solution of acetic acid, selected on the basis of single-factor test by acetic acid concentration, extraction temperature and extraction time for response surface analysis.The phytic acid content (mass fraction) in extract is for response value, established the quadratic polynomial model of phytic acid and determined the optimum conditions of phytic acid is: acid concentration of 4.4%, extraction temperature 41℃, extraction time 2h and the phytic acid content in extract is 1.64%.

  16. Kunitz trypsin Inhibitor and phytic acid levels in conventional and genetically modified soybean seeds from Londrina and Ponta Grossa, South Brazil

    Directory of Open Access Journals (Sweden)

    Olívio F. Galão

    2014-09-01

    Full Text Available Differences in the levels of antinutritional components - Kunitz trypsin inhibitor and phytic acid - were determined for conventional and genetically modified soybean cultivated in Londrina and Ponta Grossa, South Brazil. Trypsin inhibitor levels in the conventional cultivars of Londrina and Ponta Grossa varied from 14.56 mg g-1 (BRS 267 to 20.40 mg g-1 (BRS 261 and from 13.51 mg g-1 (BRS 232 to 19.65 mg g-1 (BRS 268, respectively, whereas in the genetically modified (GM cultivars, they were found to be 14.16 mg g-1 (BRS 242 RR to 18.21 mg g-1 (BRS 255 RR for Londrina, and 13.68 mg g-1 (BRS 244 RR to 18.73 mg g-1 (BRS 256 RR for Ponta Grossa. Average levels of phytic acid in the Londrina’s conventional and GM samples were estimated as 2.05 and 1.78 mg g-1, respectively, and in the Ponta Grossa conventional and GM samples, the respective average values were lower, 1.60 and 1.51 mg g-1.

  17. Phytic acid enhances the oral absorption of isorhamnetin, quercetin, and kaempferol in total flavones of Hippophae rhamnoides L.

    Science.gov (United States)

    Xie, Yan; Luo, Huilin; Duan, Jingze; Hong, Chao; Ma, Ping; Li, Guowen; Zhang, Tong; Wu, Tao; Ji, Guang

    2014-03-01

    Total flavones of Hippophae rhamnoides L. (TFH) have a clinical use in the treatment of cardiac disease. The pharmacological effects of TFH are attributed to its major flavonoid components, isorhamnetin, kaempferol, and quercetin. However, poor oral bioavailability of these flavonoids limits the clinical applications of TFH. This study explores phytic acid (IP6) enhancement of the oral absorption in rats of isorhamnetin, kaempferol, and quercetin in TFH. In vitro Caco-2 cell experiments and in vivo pharmacokinetic studies were performed to investigate the effects of IP6. The aqueous solubility and lipophilicity of isorhamnetin, quercetin, and kaempferol were determined with and without IP6, and mucosal epithelial damage resulting from IP6 addition was evaluated by MTT assays and morphology observations. The Papp of isorhamnetin, kaempferol, and quercetin was improved 2.03-, 1.69-, and 2.11-fold in the presence of 333 μg/mL of IP6, respectively. Water solubility was increased 22.75-, 15.15-, and 12.86-fold for isorhamnetin, kaempferol, and quercetin, respectively, in the presence of 20mg/mL IP6. The lipophilicity of the three flavonoids was slightly decreased, but their hydrophilicity was increased after the addition of IP6 in the water phase as the logP values of isorhamnetin, kaempferol, and quercetin decreased from 2.38±0.12 to 1.64±0.02, from 2.57±0.20 to 2.01±0.04, and from 2.39±0.12 to 1.15±0.01, respectively. The absorption enhancement ratios were 3.21 for isorhamnetin, 2.98 for kaempferol, and 1.64 for quercetin with co-administration of IP6 (200 mg/kg) in rats. In addition, IP6 (200 mg/kg, oral) caused neither significant irritation to the rat intestines nor cytotoxicity (400 μg/mL) in Caco-2 cells. The oral bioavailability of isorhamnetin, kaempferol, and quercetin in TFH was enhanced by the co-administration of IP6. The main mechanisms are related to their enhanced aqueous solubility and permeability in the presence of IP6. In summary, IP6 is a

  18. Inositol hexa phosphoric acid (phytic acid), a nutraceuticals, attenuates iron-induced oxidative stress and alleviates liver injury in iron overloaded mice.

    Science.gov (United States)

    Bhowmik, Anwesha; Ojha, Durbadal; Goswami, Debayan; Das, Rashmi; Chandra, Nidhi S; Chatterjee, Tapan K; Chakravarty, Amit; Chakravarty, Sudipa; Chattopadhyay, Debprasad

    2017-03-01

    Inositol hexa phosphoric acid (IP6) or Phytic acid, a natural antioxidant of some leguminous plants, known to act as a protective agent for seed storage in plants by suppressing iron catalyzed oxidative process. Following the same mechanism, we have tested the effect of IP6 on iron overloaded in vitro oxidative stress, and studied it's in vivo hepatoprotective ability in iron-dextran (injection)-induced iron overloaded liver injury in mice (intraperitoneal). Our results showed that IP6 had in vitro iron chelation (IC50 38.4μg/ml) activity, with the inhibition of iron-induced lipid peroxidation (IC50 552μg/ml), and deoxyribose sugar degrading hydroxyl radicals (IC50 448.6μg/ml). Oral administration of IP6 (0-200mg/kg) revealed significant decrease in biochemical markers such as serum iron, total iron binding, serum ferritin and serum enzymes. Histopathology of liver stained with hematoxylin-eosin and Prussian blue showed reduced hepatocellular necrosis, ballooning and inflammation, indicating the restoration of normal cellular integrity. Interestingly, the IP6 was found to down-regulate the mRNA expression of tumor necrosis factor (TNF)-α, Interleukin (IL)-1β, and IL-6 in iron overloaded liver tissues. Thus, we provide an insight that IP6, a natural food component, can serve as an iron chelator against iron overload diseases like Thalassemia, and also as a dietary hepatoprotective supplement.

  19. Phytic acid as a corrosion inhibitor for 235 steel in NaCl solution%植酸自组装膜对Q235钢在氯化钠溶液中的缓蚀作用

    Institute of Scientific and Technical Information of China (English)

    王凤平; 胡隋军; 唐丽娜

    2014-01-01

    The corrosion inhibition of phytic acid on Q235 steel in NaCl solution was investigated by weight loss technique ,scanning electronic microscopy and the energy spectrum of X rays .The results show that inhibition efficiency of phytic acid on Q 235 steel in NaCl solution is obviously different at different pH .Phytic acid can accelerate the corrosion rate of Q 235 steel under acidic condition .But under neutral and alkaline condition ,phytic acid has excellent corrosion inhibition for 235steel in NaCl solution .Nevertheless ,the corrosion inhibition efficiency of phytic acid for Q235 steel in NaCl solu-tion increases with increasing phytic acid concentration under neutral and alkaline condition .If the concentration of phytic acid is 0 .5% ,the inhibition efficiency is more than 90% .%从植物中提取的植酸作金属缓蚀剂对环境保护具有重要意义.采用失重法、扫描电子显微镜和X射线能谱研究了植酸对Q235钢在NaCl溶液中的缓蚀行为以及影响因素.失重实验结果表明,不同p H值植酸对Q 235钢的缓蚀效果明显不同,在酸性条件下植酸加速Q235钢的腐蚀,在中性及碱性条件下,植酸对Q235钢的腐蚀具有明显的抑制作用.中性或碱性条件下,NaCl溶液中植酸对Q235钢缓蚀效率随植酸浓度的增加而增加,植酸浓度达0.5%以上时,缓蚀效率高达90%以上.扫描电镜结果表明,植酸处理后的Q235钢表面生成膜在不同pH下明显不同,碱性条件下的生成膜明显好于酸性条件下的生成膜.

  20. Synthesis of Germanium(Ⅳ) Complex with Phytic Acid and Investigation on its Toxicities%植酸锗配合物的合成及其毒性研究

    Institute of Scientific and Technical Information of China (English)

    高敬群; 范大民; 张向东; 王君; 刘宏生

    2001-01-01

    In this Paper it was introduced the synthesis and composition of the germanium(Ⅵ) complex with phytic acid and the investigation on its toxicity by animal experiments.The experimental results showed that the germanium(Ⅵ) complex with phytic acid has a relatively lower toxicity as phytic acid has.The possibilities of asing the germanium(Ⅵ) complex with phytic acid as the additives of food,cosmetics and medicine were investigated.%介绍了植酸锗配合物的合成和组成以及通过动物实验研究了它的毒性.实验结果表明植酸锗有着类似于植酸的较低的毒性,由此,又探讨了植酸锗作为食品和化妆品的添加剂以及作为医疗保健品主要成分的可靠性.

  1. Hulled and hull-less barley cultivars with the genetic trait for low-phytic acid increased the apparent digestibility of phosphorus and calcium in diets for young swine

    Science.gov (United States)

    A 35-d experiment was conducted using 63 crossbred pigs (35 barrows and 28 gilts) with an average initial BW of 7.0 kg and age of 28 d to evaluate the efficacy of the low-phytic acid (LPA) genetic trait in hulled and hull-less barley cultivars. The hulled cultivars were Harrington normal barley (NB)...

  2. Optimization of potassium oxalate extracting of phytic acid from rapeseed cake by response surface methodology%响应面法优化草酸钾溶液提取菜籽饼中植酸工艺

    Institute of Scientific and Technical Information of China (English)

    汤务霞; 杨虎; 刘超; 欧阳磊; 阙晓莉

    2012-01-01

    Rapeseed cake is a good raw material of extracting phytic acid, and phytic acid has unique biological activities. The solution of potassium oxalate was used to extract phytic acid from rapeseed cake. On the basis of single factors experiments, the extraction technic was optimized by response surface methodology , and a quadratic polynomial mathematical model was established. The optimal conditions were as follows; pH 8, ratio of liquid to solid 14:1, concentration of potassium oxalate 0.075 mol/L, extraction time 5 h. Under the optimal conditions, the yield of phytic acid was 1. 81%.%菜籽饼是提取植酸的良好原料,植酸具有独特的生物活性.采用草酸钾溶液提取菜籽饼中的植酸,在单因素试验的基础上,通过响应面法优化植酸提取工艺,建立了以草酸钾溶液提取植酸的二次多项数学模型,并得到最佳工艺条件为:pH 8,液料比14∶1,草酸钾溶液浓度0.075 mol/L,提取时间5h.经过验证试验,在最佳工艺条件下,植酸得率为1.81%.

  3. Biosynthesis, Metabolism and Physiological Roles of Phytic Acid in Higher Plants%高等植物体中植酸合成、代谢及其生理作用

    Institute of Scientific and Technical Information of China (English)

    靳晓琳; 王新坤; 杨润强; 仲磊; 顾振新

    2014-01-01

    本文对植酸及其存在形式、代谢、调控及在高等植物内的生理作用作了介绍。%This article described the existing form, metabolism, regulation and the possible physiological roles of phytic acid in higher plant.

  4. 镁-锂-铝合金稀土-植酸转化膜的研究%A Study of RE-Phytic Acid Conversion Coating on Mg-Li-AI Alloy

    Institute of Scientific and Technical Information of China (English)

    黄晓梅; 张栓; 朱俊生

    2012-01-01

    Double layer coating on Mg-Li-Al alloy was studied as follows: A rare earth conversion coating was formed at first in optimal conditions, and then the rare earth conversion coating was submerged in phytic acid solution to form RE-phytic acid conversion coating. The effects of phytic acid concentration, immersion time and soaking temperature on RE-phytic acid conversion coating of the Mg-Li-Al alloy were investigated.%对镁-锂-铝合金表面进行了双层膜研究,即:先按照最佳稀土转化膜工艺形成一层稀土转化膜,再将稀土转化膜浸入植酸溶液中形成稀土-植酸转化膜.研究了植酸的体积分数、浸泡时间及浸泡温度对镁锂-铝合金稀土-植酸转化膜性能的影响.

  5. 黄铜表面富植酸钝化工艺研究%Passivation Technology Research of Rich-Phytic Acid for Brass-Strip

    Institute of Scientific and Technical Information of China (English)

    蔡薇; 刘仁辉; 魏仕勇; 刘羽飞

    2012-01-01

    The effect of passivation conditions (temperature, passivation time and pH value of passivation solution) on the corrosion resistance of passivation film treated by passivator of rich-phytic acid (50% , mass fraction) 8 ml·L-1, hydrogen peroxide (30%, mass fraction) 30 ml·L-1, boric acid 5 g·L-1, polyethylene glycol 15 ml·L-1, additive 4 g·L-1 was investigated. Samples were made of cold-rolled strip of HA172-2.5-1 alloy. The corrosion resistance of passivation film was detected by nitric acid dripping. The results showed that the optimum condition of passivation by rich-phytic acid passivation solution was at the range of 35 ~ 40 ℃, pH 2.5 dipping for about 60 s. Photos of SEM showed that the passivation film was uniform and compact. The weight loss test showed that the average corrosion rate of samples treated by rich-phytic acid passivator was 0. 0054 g·m-2·h-1, that was equal to the rate (0.0040 g·m-2·h-1) of dichromate treated samples, and much lower than the corrosion rate (0.0376 g·m-2·h-1) of samples without passivation treatment. It meant that rich-phytic acid passivator protected brass strip well. 12 h of salt spray test showed that the dichromate treated samples changed brown with a lot of corrosion spots, and rich phytic acid treated samples turned to golden yellow with only a few spots. It showed that brass strip processed by rich phytic acid had better anti-color capability than those dichromate treated samples. The rich phytic acid passivator was environment protection without chromium ions.%采用植酸(50%,质量分数)8 ml·L-1,双氧水(30%,质量分数)30 ml·L-1,硼酸5 g·L-1,聚乙二醇15 ml·L-1,添加剂4 g·L-1钝化液对冷轧HA172-2.5-1黄铜带材进行钝化处理,探讨工艺条件(钝化温度、钝化时间和钝化液pH值)对富植酸钝化膜耐蚀性的影响.通过硝酸溶液点滴腐蚀试验对黄铜表面富植酸钝化膜的耐腐蚀性能进行研究.结果表明,富植酸钝化膜的最佳工艺条

  6. 小麦粉中添加植酸对馒头品质的影响%Effects of Added Phytic Acid in Wheat Flour on Quality of Chinese Steamed Bread

    Institute of Scientific and Technical Information of China (English)

    吴澎; 陈建省; 段纯明; 田纪春

    2011-01-01

    As a new natural food additive, phytic acid is used as antioxidant, antiseptic, ferment accelerant and chelant in food industry. In the experiment, three kinds of wheat flour with three different gluten gradients were used and added with the phytic acid with the proportion of 0.005% ,0.025% ,0.05% ,0.1% ,0. 15% and 0.2% (phytic acid:wheat flour), and then were used to make Chinese steamed bread, so as to measure its structural features and evaluate the steamed bread. The result indicated that with the increase in the proportion of phytic acid, the textural properties of steamed breads had a little changes, while the volume and the total score of steamed breads reduced slightly, but the color of steamed breads improved slightly. Significant negative correlations were detected between hardness, cohesiveness, adhesiveness, chewiness, volume with the increase proportion of phytic acid, while no significant correlations were identified between other properties with the increase in the proportion of phytic acid. In conclusion,there was no significant influence of the addition of phytic acid on the quality of steamed bread.%植酸是一种新型的天然食品添加剂,作为抗氧化剂、防腐剂、发酵促进剂或螯合剂广泛用于食品工业.试验利用3个不同筋力梯度的小麦粉,按0.005%、0.025%、0.05%、0.1%、0.15%、0.20%(植酸与制造馒头小麦粉质量比)的比例添加植酸后制作馒头,测定其质构特性并做馒头感官评价试验.结果表明,随着植酸添加比例的增加,馒头的体积和评分值均有小幅度下降,但是无论馒头瓤和皮的色差都有小幅度的增白现象.硬度、黏着性、胶着性、咀嚼性和体积与植酸添加量呈显著线性负相关,其余性状与植酸添加量的相关性不显著.但总的来说,添加植酸对馒头的品质特性没有起到显著性的影响.

  7. Application of non-toxic phytic acid in metal protection%植酸在金属防腐中的应用研究

    Institute of Scientific and Technical Information of China (English)

    刘泓; 沈月敏

    2009-01-01

    用失重法研究了植酸对金属的阻蚀性.分别以0.1 mol/L HCl、0.1 mol/L NaCl或0.1 mol/L NaOH为介质,配置不同植酸浓度的 (空白自来水,0.5%,1%,1.5%,2%)溶液,并与相同介质下1.5%亚硝酸钠或1.5%磷酸二氢锌的溶液相比较.实验表明,对于铝、锌和铁三种金属,植酸对铝的防腐率最为显著,其中尤以植酸在NaCl介质中对铝的防腐性能最优,最佳防腐浓度为0.5%;对于锌、铁,防腐作用最佳的是空白自来水介质的1.5%磷酸二氢锌钝化液.%The application of phytic acid in metal protection was researched by weight-loss method.Using 0.1 mol/L HCl,0.1 mol/L NaCl or 0.1 mol/L NaOH respectively as the medium,different concentrations of phytic acid(blank water,0.5%,1%,1.5%,2%) were prepared,compared with the solution of 1.5% NaNO2 or 1.5% Zn(H_2PO_4)_2.The result of the experiment is as follows:for Al, Zn and Fe,the corrosion rate of phytic acid to Al is the best,and its best media is NaCl,the best concentration is 0.5%.For Zn and Fe,its best media is 1.5% Zn(H_2PO_4)_2 of blank water.

  8. Phytic Acid Protects against 6-Hydroxydopamine-Induced Dopaminergic Neuron Apoptosis in Normal and Iron Excess Conditions in a Cell Culture Model.

    Science.gov (United States)

    Xu, Qi; Kanthasamy, Anumantha G; Reddy, Manju B

    2011-02-07

    Iron may play an important role in Parkinson's disease (PD) since it can induce oxidative stress-dependent neurodegeneration. The objective of this study was to determine whether the iron chelator, phytic acid (IP6) can protect against 6-hydroxydopamine- (6-OHDA-) induced apoptosis in immortalized rat mesencephalic dopaminergic cells under normal and iron-excess conditions. Caspase-3 activity was increased about 6-fold after 6-OHDA treatment (compared to control; P IP6 pretreatment decreased it by 38% (P IP6 pretreatment. Under iron-excess condition, a 6-fold increase in caspase-3 activity (P IP6. Together, our data suggest that IP6 protects against 6-OHDA-induced cell apoptosis in both normal and iron-excess conditions, and IP6 may offer neuroprotection in PD.

  9. Determination of lead(Ⅱ) using sodium phytic acid modified glassy carbon electrode%植酸钠修饰玻碳电极测定铅(Ⅱ)

    Institute of Scientific and Technical Information of China (English)

    阮勇; 朱艳; 龙丽; 黄杉生

    2012-01-01

      A sodium phytic acid modified electrode was prepared by coating solution containing phytic acid on the surface of glassy carbon electrode. The electrochemical behavior of Pb2+at modified electrode were investigated by electrochemical methods and the measurement conditions were optimized. Compared with the bare glassy carbon electrode, the modified electrode showed good characteristics in the response current for Pb2+, lower detection limit and fast analysis. Under the best experimental condition, the peak current of Pb2+has good linearity with concentra-tion in the range of 1.2×10-6~1.2×10-5 mol/L with a detection limit of 8×10-7 mol/L. The modified electrode pre-pared simply showed good stability%  采用涂覆法制备了植酸钠修饰电极,采用电化学方法研究了Pb2+在修饰玻碳电极上的电化学行为,对测定条件进行了优化.与裸玻碳电极相比较,Pb2+在修饰电极上的峰电流强度明显提高,该方法检出限低、分析速度快.在最佳实验条件下,Pb2+在1.2×10-6~1.2×10-5 mol/L的浓度范围内与其峰电流呈良好的线性关系,检出限为8.0×10-7 mol/L.该修饰电极制备简单,稳定性好

  10. Identification of genes necessary for wild-type levels of seed phytic acid in Arabidopsis thaliana using a reverse genetics approach.

    Science.gov (United States)

    Kim, Sang-Ic; Tai, Thomas H

    2011-08-01

    The majority of phosphorus (P) in seeds is found in phytic acid (InsP(6)) which accumulates as the mixed salt phytate. InsP(6) is generally considered to be an anti-nutrient and the development of low phytic acid (lpa) seed crops is of significant interest. We have employed a reverse genetics approach to examine the impact of disrupting genes involved in inositol phosphate metabolism on Arabidopsis seed InsP(6) levels. Our analysis revealed that knockout mutations in three genes (AtITPK1, AtITPK4, and AtMIK/At5g58730) reduced seed InsP(6) in addition to knockouts of four previously reported genes (AtIPK1, AtIPK2β, AtMRP5, and At5g60760). Seeds of these lpa mutants also exhibited reduced germination under various stress conditions. The greatest reduction in InsP(6) (>70%) was observed in atmrp5 seeds which were also among the least sensitive to the stresses examined. Expression analysis of the lpa genes revealed three distinct patterns in developing siliques consistent with their presumed roles. Disruption of each lpa gene resulted in changes in the expression in some of the other lpa genes indicating that transcription of lpa genes is modulated by other constituents of InsP(6) metabolism. While all the lpa genes represent possible targets for genetic engineering of low phytate seed crops, mutations in AtMRP5, AtMIK, and At5g60760 may be most successful for conventional approaches such as mutation breeding.

  11. Effects of Individual and Combined 1-MCP and Phytic Acid Treatments on Physiological Characteristics of Postharvest Strawberry%1-MCP与植酸处理对草莓果实采后生理品质的影响

    Institute of Scientific and Technical Information of China (English)

    祝美云; 白欢; 魏征; 李雅善

    2011-01-01

    采用1-甲基环丙烯(1-MCP)和植酸对草莓进行不同处理,通过追踪测定其贮藏期间的质量损失率、硬度、可溶性固形物(soluble solids content,SSC)、呼吸强度、维生素C(VC)、可滴定酸(titratable acidity,TA)和相对电导率等指标来研究不同处理对草莓的保鲜效果。结果表明:1-MCP和植酸处理可有效降低草莓的质量损失率和呼吸强度,推迟SSC出现峰值的时间,延缓其后熟衰老过程,抑制其硬度、TA和VC含量的下降;同时,1-MCP和植酸复合处理比两者各自的单独处理都具有更好的效果,能显著改善草莓在贮藏期间的果实品质。%In order to reveal the fresh-keeping effects of 1-MCP and phytic acid on postharvest strawberry,strawberry was treated with 1-MCP alone,0.1% phytic acid alone and both of them(first 1-MCP and then 0.1% phytic acid) before storage at(4 ±1) ℃,and the changes of weight loss rate,firmness,soluble solid content(SSC),respiration intensity,vitamin C,titratable acidity(TA) and relative conductivity were measured during the storage period of 14 days.The results indicated that 1-MCP and phytic acid could effectively reduce the weight loss rate and respiration intensity of strawberry,delay the occurrence of SSC peak,ripening and senescence,and inhibit the decrease of fruit firmness and the content of TA and VC.Better results were achieved by the combined use of 1-MCP and phytic acid than their separate use.The quality of strawberry could be significantly improved by the combined use of 1-MCP and phytic acid during storage.

  12. 植酸对镁锂合金微弧氧化膜的影响%Effects of Phytic Acid on Coating Properties Obtained by Micro-arc Oxidation on Mg-Li Alloy

    Institute of Scientific and Technical Information of China (English)

    王丽萍; 徐用军; 姚忠平

    2012-01-01

    A ceramic coating was formed on the surface of Mg-Li alloy by micro-arc oxidation technology, and the surface and section morphology, element composition and corrosion resistance of the film were examined by scanning electron microscopy (SEM) , energy dispersive X-ray spectroscopy (EDS) , polarization curves. With trie addition of phytic acid to the basic electrolyte, phytic acid was successfully doped into the ceramic coatings by micro-arc oxidation. Effects of phytic acid concentration on the properties of ceramic film were discussed. The results show that thickness of the coatings increases with the phytic acid concentration increasing and surface morphology of the film improves by degrees. C and P elements are successfully doped into the ceramic coatings. With increasing of the phytic acid concentration, C and P content in the coatings increase while Mg content decreases. The coating formed in the solution containing 4g/L phytic acid exhibits the best uniformity, density and general corrosion resistance.%采用微弧氧化技术在镁锂合金表面制备了陶瓷膜,通过扫描电镜、X射线能谱分析、动电位极化曲线等测试技术研究了陶瓷膜的表面和截面形貌、元素组成以及耐蚀性能.采用在基本电解液里添加植酸的方法将其引入陶瓷膜,并讨论植酸质量浓度对成膜过程和陶瓷膜性能的影响.研究结果表明:陶瓷膜厚度随电解液中植酸浓度的增加而变大,膜层表面形貌逐渐改善;膜层中成功地引入了碳、磷元素,随植酸浓度增加,膜层中的碳、磷元素含量增大,镁元素含量降低;植酸的质量浓度为4g/L时所制备的陶瓷膜均匀细致,耐腐蚀性能最好.

  13. Further improvement of flame retardancy of polyaniline-deposited paper composite through using phytic acid as dopant or co-dopant.

    Science.gov (United States)

    Zhou, Yang; Ding, Chunyue; Qian, Xueren; An, Xianhui

    2015-01-22

    Polyaniline (PANI)-deposited electrically conductive and flame retardant paper composite was prepared using phytic acid (PA) as dopant or co-dopant. PA as doping acid greatly improved the flame retardancy of PANI-deposited paper composite whilst the conductivity was lower compared with using 5-sulfosalicylic acid (SSA) as doping acid. Lower temperature was favorable to obtain PANI-deposited paper composite with both higher conductivity and better flame retardancy. Conductivity of PANI-deposited paper composite increased with increase of doping acid concentration and the suitable PA concentration range was 0.15-0.3 mol/L depending on the requirement of conductivity and flame retardancy. The PANI-deposited paper composite was characterized by SEM, TGA and XPS. The outstanding flame retardancy of PA-doped paper composite was caused by the synergetic effect of PANI coating and H3PO4. Both higher flame retardancy and higher conductivity of PANI-deposited paper composite were obtained by co-doping of SSA with PA.

  14. Estudo da estabilidade do complexo ácido fítico e o íon Ni(II Study of stability of phytic acid with Ni(II complex

    Directory of Open Access Journals (Sweden)

    Ligia De Carli

    2006-03-01

    Full Text Available A técnica de titulação potenciométrica foi utilizada para verificar as propriedades ácida-base do ácido fítico [1,2,3,4,5,6-hexaquis(dihidrogenofosfato-mio-inositol] e do complexo ácido fítico e Ni(II, em solução aquosa, em temperatura e força iônica constantes. Para avaliar o comportamento térmico e a complexação do ácido fítico com o íon Ni(II foram realizadas análises de Termogravimetria (TG, Termogravimetria Derivada (DTG, Calorimetria Exploratória Diferencial (DSC e estudos de Espectrofotometria de Infravermelho. Foram obtidas oito constantes de protonação da amostra de ácido fítico na forma de sal de dipotássio e sete constantes de estabilidade do complexo ácido fítico e Ni(II. As reações de protonação e de formação ocorrem na faixa de pH de 2,0 a 11,0. Os dados obtidos mostram que o ácido fítico encontra-se totalmente deprotonado em pH 12,0 no qual a espécie ML (um ligante para um íon metálico encontra-se totalmente formada no mesmo valor de pH. Os resultados obtidos por TG e DSC revelaram tanto para o ácido fítico como para o complexo boa estabilidade até a temperatura próxima a 200ºC. Por TG, DTG e DSC conclui-se também que a estequiometria do complexo estudado foi de um mol de ligante para um mol de íon metálico. A Espectrofotometria de Infravermelho comprovou a estabilidade do ácido fítico e a sua interação com o íon Ni(II.The technique of potenciometric titration was used to verify the acid-basic properties of the phytic acid, [1,2,3,4,5,6-hexakis(dihydrogen phosphate-myo-inositol] and the Phytic Acid-Ni(II complex, in aqueous solution, in constant temperature and ionic strength. To evaluate the thermal behavior end complexation of the isolated phytic acid with the Ni(II were performed analyses of thermogravimetry (TG, calorimetric scanning differential (DSC and studies Spectroscopy Infrared (IR. Eight protonation constants of the phytic acid sample as dipotassium salt were

  15. Study on extraction technology of phytic acid from Jatropha curcas L. seed cake%从麻疯树籽饼粕提取植酸工艺研究

    Institute of Scientific and Technical Information of China (English)

    马博; 黎远成; 张婷婷; 宁世灯

    2013-01-01

      为对麻疯树籽饼粕进行资源综合利用,该研究以植酸得率为考查指标,在单因素实验基础上,采用四因素三水平正交试验,优化从麻疯树籽饼粕中提取植酸工艺。结果显示,优化提取植酸工艺条件为:盐酸浓度0.8 mol/L、温度60℃、时间60 min、液料比12∶1;在该工艺条件下,植酸得率达4.80%。%Phytic acid is one of main anti–nutrient constituents in the seed cake of Jatropha curcas L. In order to improve the forage quality,the extraction technique of phytic acid from Jatropha curcas L. cake was optimized by using an orthogonal test with 4 factors and 3 levels,based on the single test and selecting the extraction ration as index. The optimum conditions were determined as follows:hydrochloric acid at 0.8 mol/L,temperature at 60℃,extraction time at 60min and the ration of liquid to materials at 12∶1. The extraction yield of phytic acid was 4.80%.

  16. In vitro (α-glucosidase and α-amylase inhibition) and in vivo antidiabetic property of phytic acid (IP6) in streptozotocin- nicotinamide-induced type 2 diabetes mellitus (NIDDM) in rats.

    Science.gov (United States)

    Kuppusamy, Asokkumar; Muthusamy, Umamaheswari; Thirumalaisamy, Sivashanmugam Andichetiar; Varadharajan, Subhadradevi; Ramasamy, Kalyanasubramaniam; Ramanathan, Sambathkumar

    2011-01-01

    Phytic acid, inositol hexaphosphate (IP6) a natural plant constituent and antioxidant exhibits protective action in carcinogenesis, Alzheimer's, hypercholesterolemia, diabetes and inflammations when taken in diet. The aim of this study is to evaluate effect of phytic acid in streptozotocin (STZ)-nicotinamide-induced type 2 diabetes in rats. The STZ-nicotinamide induced diabetic rats were orally treated with vehicle (2%w/v Tween 80), glimepiride (2.5 mg/kg) and IP6 (650 mg/kg) for 28 days. The blood glucose level, body weight, glycosylated haemoglobin (HbA1C), lipid profile, lipid peroxidation, antioxidant status (liver and small intestine) was measured and compared with control. Xanthine dehydrogenase (XDH) and xanthine (XO) activity was measured in small intestine of diabetic rats. In vitro inhibition of carbohydrate digestive enzymes (α-glucosidase and α-amylase) was also determined. IP6, significantly (PIP6 possess promising in vitro and in vivo antidiabetic activity.

  17. MB8镁合金植酸转化膜的制备及性能%Preparation and Properties of Phytic Acid Conversion Coating on MB8 Magnesium Alloy

    Institute of Scientific and Technical Information of China (English)

    张小琴; 赵晴; 王帅星; 严小梅; 张传波

    2011-01-01

    通过单因素实验优化镁合金植酸转化工艺,发现在植酸体积浓度为10 mL/L,pH=2.5,温度50℃,成膜时间20 min条件下,可在MB8镁合金表面制备出均匀一致的植酸转化膜.该膜层微观形貌与铬酸盐转化膜类似,表面呈现出均匀分布的网状微裂纹,类似于"龟裂的土地",膜层主要成分为Mg,O,P,Mn和C;动电位极化曲线测试发现MB8镁合金经植酸转化处理后,自腐蚀电流密度显著降低,表明植酸转化膜有效提高了MB8镁舍金的耐蚀性.%The phytic acid conversion technique was investigated by the single-factor experiments. It was found that the uniform coherent conversion coating on the MB8 magnesium alloy can be obtained on the condition of 10 ml/L phytic acid,pH=2.5,at 50 ℃ for 20 min. The micro-structure of the coating was similar with the chromate conversion coating, whose surface was uniform micro-crack network, similar to “the rimose land”. The main elements of the coating were Mg,O,P, Mn and C. The potentiodynamic polarization curves showed that the corrosion current density reduced greatly when the MB8 magnesium alloy was treated through phytic acid conversion technique. Which indicated that the phytic acid conversion coating improved effectively the corrosion resistance of MB8 magnesium alloy.

  18. 植酸对芦笋采后贮藏过程中生理变化及酶类的影响%Effect of the Phytic Acid on the Physiological Change and Enzyme of Post-harvest Asparagus

    Institute of Scientific and Technical Information of China (English)

    魏云潇; 何良兴; 谢灵燕

    2011-01-01

    [Objective] The effect of the phytic acid on the physiological change and enzyme of post-harvest asparagus was analyzed. [ Method] The content of chlorophyll, lignin, crude fiber, total soluble sugar and sucrose, and the activity of POD, CAT and PPO of the asparagus, treated with the phytic acid(0. 1% , 0.2% and 0.5% ) , were tested. [ Result] The application of the phytic acid could inhibit the decline in the content of chlorophyll, delay the increment in the content of total soluble sugar, lignin and crude fiber, and promote the formation of sugar. And also, it could intensify the activity of POD, PPO and CAT, which would improve the quality of asparagus through its senescence delaying. [Conclusion] The application of the phytic acid could better improve quality and prolong shift time of asparagus.%[目的]探讨植酸对芦笋采后贮藏过程中生理变化及酶类的影响.[方法]采用植酸3个浓度梯度(0.1%、0.2%、0.5%)处理芦笋,测定叶绿素、纤维素、木质素、可溶性总糖和蔗糖含量,以及POD、CAT、PPO活性的变化.[结果]植酸抑制芦笋叶绿素含量的下降,延缓木质素、粗纤维和可溶性总糖含量的上升,刺激蔗糖含量的升高,有效提高了芦笋CAT、POD和PPO活性,延缓了衰老,保持了绿芦笋的鲜嫩品质.[结论]植酸处理后能较好地保持芦笋的品质,延长保鲜期.

  19. 镁合金的微观结构对其上制备植酸转化膜的影响%The Effect of Microstructure of Magnesium Alloys on Preparation of Phytic Acid Conversion Coatings

    Institute of Scientific and Technical Information of China (English)

    杨旭; 李兰兰; 贺建; 张丁非; 潘复生

    2012-01-01

    采用浸没法,以纯Mg及AZ91D,AZ61,ZK60,ZM21镁合金为基体材料,在植酸水溶液中制备植酸转化膜.观察了植酸转化膜的形貌,分析了转化膜的元素组成及浓度分布,进而研究了镁合金的微观结构对制备表面植酸转化膜的影响.结果表明:合金元素对植酸转化膜的形成起着决定性的作用,高价合金元素(Al和Zr)比低价合金元素(Zn和Mn)更有利于植酸转化膜的形成,以固溶体存在的Al比以化合态(Mg17Al12)存在的Al更有利于转化膜的形成.%To study the influence of microstructure of magnesium alloys on preparation of phytic acid conversion coatings, the phytic acid coatings were prepared on AZ91D, AZ61, ZK60, ZM21 magnesium alloys in phytic acid aqueous solution by immersion method, and pure magnesium were used to compare. The surface morphologies of conversion coating were observed, and surface element compositions and concentration distribution of conversion coating were analyzed, and the effect of the microstructure on preparation of phytic acid conversion coating was studied. The results show that alloying elements play a definitive role in forming conversion coatings. Tetravalent and trivalent alloying elements are more helpful than divalent alloying elements. Alloying elements dissolved in the magnesium matrix (alpha-phase) are more beneficial for forming coatings than that in the second-phase.

  20. Effects of dietary phytic acid on the growth and digestive enzyme activities of Japanese flounder,Paralichthys olivaceus%植酸对牙鲆生长和消化酶活性的影响

    Institute of Scientific and Technical Information of China (English)

    蔡英华; 麦康森; 艾庆辉; 张文兵; 刘付志国; 谭北平; 陈伟

    2009-01-01

    A trial was conducted to evaluate the effects of dietary phytic acid on the growth,digestive enzyme activities,apparent digestibility coefficients of Japanese flounder(Paralichthys olivaceus).Four practical diets were formulated to contain 0.04%(control),0.21%,0.44% and 0.81% phytic acid.Each diet was randomly fed to triplicate tanks,and each tank was stocked with 35 fish(initial weight about 2.58 g±0.01 g).Fish were fed twice daily(08:00,17:00)to satiate for 10 weeks.The results showed that feed intake(2.4%,2.5% and 2.4%)in fish fed the diets supplemented with phytic acid were significantly higher than that of the control(2.1%)(P<0.05),but no significant difference was observed among the fish fed the diets supplemented with phytic acid(P>0.05).No significant difference in both amylase activities and protease activities of liver,intestine and stomach was found among dietary treatments except pepsin.The pepsin activities in fish fed the phytic acid supplemented diets(59.47,61.27 and 64.82 U/mg protein)were significantly lower than that of the control(79.34 U/mg protein).The apparent digestibility coefficient of dry matter and protein decreased significantly with increasing dietary phytic acid.Specific growth rate decreased with increasing dietary phytic acid,but no significant difference was observed among dietary treatments.The feed conversion efficiency(74.0%,73.7% and 69.2%)in fish fed the phytic acid supplemented diets was significantly lower than that of the control(84.0%),but no significant difference was observed among fish fed the phytic acid supplemented diets.The similar trend was found in protein efficiency ratio and net protein utilization.The protein efficiency ratio and net protein utilization in fish fed the diet with 0.81 %phytic acid were significantly lower than those of the control.The results of the present study indicated that dietary phytic acid significantly depressed the growth and pepsin activity of Japanese flounder with increasing

  1. Genetic Analysis and Identification of Maize (Zea mays L.)Low Phytic Acid Inbred Lines%低植酸玉米自交系的筛选及其遗传分析

    Institute of Scientific and Technical Information of China (English)

    马磊; 李盼; 陈哲; 赵永锋; 祝丽英; 黄亚群; 陈景堂

    2011-01-01

    [目的]筛选获得低植酸玉米自交系,研究低植酸性状的遗传规律,为低植酸玉米杂交种的选育提供依据.[方法]通过钼酸铵显色法对100份玉米自交系进行测定,将筛选得到的低植酸自交系鲁原92与已知低植酸突变体Lpa/Ipa241、Lpa//Lpa241和齐319进行杂交,分析低植酸性状的遗传;并用钼酸铵显色法和植酸含量国标测定法对6个不同来源的齐319、鲁原92及其杂交种进行植酸含量比较分析,同时通过发芽试验对齐319、齐319×Lpa241/1pa241、鲁原92×Lpa241/1pa241、鲁原92×齐319苗期表现进行鉴定分析.[结果]钼酸铵显色法显示,鲁原92的无机磷含量达到0.93μg·mg-1水平,初步确定属于低植酸自交系;其低植酸性状受隐性单基因控制,可能与1pa241等位;齐319在植酸性状上表现杂合性,存在苗期纯合致死现象;齐319×Lpa241/1pa241,鲁原92×Lpa241/1pa241,鲁原92×齐319 F2苗期纯合致死比例相近;不同来源的同一自交系间植酸含量存在显著差异.[结论]筛选得到低植酸自交系1个,分析其低植酸性状为隐性单基因控制,且与1pa241等位.%[Objective]The purpose of this experiment was to provide a theoretical basis for selective breeding of the low phytic acid maize hybrid through screening low phytic acid materials from general maize inbred lines and study the genetic characteristics of low phytic acid trait.[Method]In this study, through using the colorinmetric assay for high inorganic phosphorus (HIP), kernels from 100 maize inbred lines were indirectly screened respectively.To analyze the genetic characteristics of low-phytic acid trait, the screened Luyuan 92 was hybridized with known low phytic acid mutants Lpa/lpa241, Lpa/Lpa241 and low-phytic acid inbred line Qi 319.Also the phytic acid content of two inbred lines Qi 319, Luyuan 92 and their reciprocal hybrids from six different origins were analysed by modified colorimetric assay and GB technique.The phenotypes of

  2. 植酸浓度对AZ31B镁合金植酸转化膜防腐性能的影响%Influence of Phytic Acid Concentration on Corrosion Resistance of Phytic Acid Conversion Coating on AZ31B Magnesium Alloy

    Institute of Scientific and Technical Information of China (English)

    高焕方; 张胜涛; 赵波; 刘益风; 邹勇

    2011-01-01

    在不同浓度的植酸溶液中制备了AZ31B镁合金植酸转化试样,并应用析氢实验及Tafel极化曲线测试其防腐性能,使用SEM,EDS,FTIR观察转化膜形貌、元素组成及官能团构成.结果表明:植酸溶液的浓度对植酸转化试样的防腐性能具有较大的影响,C=4.0g·L-1时所制备的转化试样具有最佳的防腐性能,电流密度较未处理试样降低了2个数量级.此外,植酸转化膜主要由Mg,Al,Zn,O,P等元素组成,并含有PO34-,HPO24-,OH-基团,转化膜存在一定的裂纹,且裂纹处仍有很薄的一层植酸转化膜.%The phytic acid conversion coatings on AZ31B magnesium alloy were formed in different phytic acid concentrations and the corrosion resistance of the conversion samples was studied by hydrogen evolution method and Tafel. The morphology, composition and functional groups were investigated by SEM, EDS and FTIR, respectively. The results indicate that the concentration of phytic acid has obvious influence on the corrosion resistance of the conversion samples, the conversion sample formed under C=4.0g · L-1 has the best corrosion resistance, the current density decreases about two orders than that of the untreated sample. The main elements of the coating are Mg, Al, Zn, O and P, the functional groups are PO43- , HPO42- and OH- . The conversion coating also has few cracks, and the thin coating is formed on the crack.

  3. 植酸和几种抗氧化物质对自由基清除能力的比较%A comparative study of roles of phytic acid and several antioxidants in eradication of free radicals

    Institute of Scientific and Technical Information of China (English)

    贾炎; 涂书新; 唐世荣

    2011-01-01

    Diphenylpicrylhydrazyl (DPPH) spectrocolorimetery method was used to evaluate the an-ti-oxidative capacity of phytic acid,ascorbic acid (ASA),glutathione (GSH) and vegetable extracts. The results showed that eradication of free radical of phytic acid, ASA and GSH was pH dependent. The IC50 a value describing the ability of DPPH free radical scavenging,was 1. 94×10-2(pH = 2. 0),0. 26 (pH=4. 6) and 1. 86 (pH=5. 7) for phytic acid,GSH and ASA, respectively. In addition,adding phytic acid enhanced the eradicating ability of free radical of vegetable extracts. The results suggested that phytic acid would be an important antioxidant in plants due to its high content in plants,and pose a crucial role in resistance to oxidative stress.%在离体条件下,比较研究了植酸和几种常见的抗氧化物质(抗坏血酸、谷胱甘肽)以及部分蔬菜(青椒、黄瓜、番茄、洋葱)提取物对二苯代苦味酰基(DPPH)自由基的清除作用.结果表明:体系的pH值是影响抗氧化物质自由基清除能力的重要因子;在一定的pH值条件下,植酸表现出清除DPPH自由基的能力,其IC50值为1.94×10-2 (pH 2.0),而谷胱甘肽和抗坏血酸的IC50值分别为0.26(pH 4.6)和1.86(pH 5.7).试验表明,蔬菜提取液添加植酸后的自由基清除能力明显提高.

  4. Mutations of the multi-drug resistance-associated protein ABC transporter gene 5 result in reduction of phytic acid in rice seeds.

    Science.gov (United States)

    Xu, Xiu-Hong; Zhao, Hai-Jun; Liu, Qing-Long; Frank, Thomas; Engel, Karl-Heinz; An, Gynheung; Shu, Qing-Yao

    2009-06-01

    Phytic acid (PA, myo-inositol 1,2,3,4,5,6-hexakisphosphate) is important to the nutritional quality of cereal and legume seeds. PA and its salts with micronutrient cations, such as iron and zinc, cannot be digested by humans and non-ruminant animals, and hence may affect food/feed nutritional value and cause P pollution of groundwater from animal waste. We previously developed a set of low phytic acid (LPA) rice mutant lines with the aim of increasing the nutritional quality of rice. Two of these lines, Os-lpa-XS110-2 (homozygous non-lethal) Os-lpa-XS110-3 (homozygous lethal), contain two mutant alleles of a LPA gene (hereafter XS-lpa2-1 and XS-lpa2-2, respectively). In this study, we mapped the XS-lpa2-1 gene to a region on chromosome 3 between microsatellite markers RM14360 and RM1332, where the rice orthologue (OsMRP5) of the maize lpa1 gene is located. Sequence analysis of the OsMRP5 gene revealed a single base pair change (C/G-T/A transition) in the sixth exon of XS-lpa2-1 and a 5-bp deletion in the first exon of XS-lpa2-2. OsMRP5 is expressed in both vegetative tissues and developing seeds, and the two mutations do not change the level of RNA transcription. A T-DNA insertion line, 4A-02500, in which OsMRP5 was disrupted, also showed the same high inorganic phosphorus phenotype as Os-lpa-XS110-3 and appeared to be homozygous lethal. PA is significantly reduced in Os-lpa-XS110-2 (~20%) and in 4A-02500 (~90%) seeds compared with their wild type lines, and no PA was detected in Os-lpa-XS110-3 using HPLC analysis. This evidence indicates that the OsMRP5 gene plays an important role in PA metabolism in rice seeds.

  5. Dietary phytic acid prevents fatty liver by reducing expression of hepatic lipogenic enzymes and modulates gut microflora in rats fed a high-sucrose diet.

    Science.gov (United States)

    Sekita, Ayaka; Okazaki, Yukako; Katayama, Tetsuyuki

    2016-06-01

    The aim of this study was to investigate the effect of phytic acid (PA) on fatty liver and gut microflora in rats fed a high-sucrose (HSC) diet. Three groups of rats were fed a high-starch (HSR) diet or an HSC diet with or without 1.02% sodium PA for 12 d. We evaluated hepatic weight, total lipids, and triacylglycerol (TG) levels, the activities and expression of hepatic lipogenic enzymes (glucose-6-phosphate dehydrogenase, malic enzyme 1, and fatty acid synthetase), and fecal microflora. The HSC diet significantly increased hepatic total lipids and TG levels, and the activities and expression of the hepatic lipogenic enzymes compared with the HSR diet. These upregulations were clearly suppressed by dietary PA. Consumption of PA elevated the fecal ratio of Lactobacillus spp. and depressed the ratio of Clostridium cocoides, and suppressed the elevation in the ratio of C. leptum induced by the HSC diet. This work showed that dietary PA ameliorates sucrose-induced fatty liver through reducing the expression of hepatic lipogenesis genes and modulates gut microflora in rats. Copyright © 2016 Elsevier Inc. All rights reserved.

  6. Minimum handling method for the analysis of phosphorous inhibitors of urolithiasis (pyrophosphate and phytic acid) in urine by SPE-ICP techniques.

    Science.gov (United States)

    Muñoz, Jose A; López-Mesas, Montserrat; Valiente, Manuel

    2010-01-25

    Pyrophosphate (PPi) and phytic acid (IP6) are natural phosphorous compounds with growing interest in the biomedical field due to their ability as potential inhibitors of urolithiasis among others. Existing methodologies for their evaluation show inconveniences mainly associated with sample treatment, matrix interferences and lack of resolution. The objective of the present work is the validation of a new method to determine both inhibitors in urine samples selectively and its application to the diagnosis of lithiasic patients. After urine purification by an off-line anion exchange solid phase extraction (SPE), based in an appropriate acidic elution gradient, the phosphorous compounds were analyzed by (31)P measurements by inductively coupled plasma mass spectrometry (ICP-MS) in the purified urine extracts. Linear range and limit of detection obtained were adequate for the analysis of the physiological amounts of the compounds in urine. The method was successfully applied to human urine samples, resulting in adequate accuracy and precision and allowing for the analysis of phosphorus inhibitors of urolithiasis in urine. The method simplicity and high sample throughput leads to a clear alternative to current determinations of the mentioned species in urine. Moreover, PPi and IP6 concentrations found in patients suffering from oxalocalcic urolithiasic were significantly lower than those for healthy controls, supporting the fact that the risk for oxalocalcic urolithiasis increases when urinary phosphorus inhibitors decrease. Thus, speciation of phosphorus inhibitors of urolithiasis in urine of stone formers can be performed, which is of unquestionable value in diagnostic, treatment and monitoring of urolithiasis.

  7. Adsorption and Desorption Properties of Phytic Acid from Rice Bran on Anion Exchange Resin%阴离子交换树脂对米糠植酸的吸附解吸性能

    Institute of Scientific and Technical Information of China (English)

    王琳; 罗建平; 查学强; 张海林; 潘利华

    2011-01-01

    通过静态和动态试验研究了6种阴离子交换树脂对植酸的吸附与解吸性能.结果表明,D201树脂对植酸的吸附交换作用较好,且在pH值为2.2时吸附能力最强,静态吸附量达到94.54 mg/g,1.5 mol/L的NaOH溶液利于植酸解吸;Freundlich吸附等温方程可以较好地描述D201树脂对植酸的等温吸附,表明吸附在常温下进行即可;D201树脂对植酸的吸附过程符合Lagergren一级速率方程,表观吸附速率常数k与植酸起始植酸浓度呈负相关关系,与温度呈正相关关系.在D201树脂对植酸的动态吸附与解吸过程中,层析柱管径、上样液浓度、上样液流速和洗脱剂流速对吸附与解吸效果影响较大.%The absorption and desorption properties of phytic acid on anion exchange resin were investigated through static and dynamic experiments. The results showed that D201 resin had the best exchange adsorption performance among all tested resins. The static absorption capacity of D201 resin reached 94. 54 mg/g when the pH value of phytic acid solution was adjusted to 2. 2, and sodium hydroxide solution of 1. 5 mol/L was beneficial to desorption. The absorption behavior of D201 resin for phytic acid obeyed the Freundlich adsorption isotherm equation, indicating that the absorption can be performed under normal temperature. The absorption kinetic data complied with Lagergren pseudo-first-order rate equation. The apparent adsorption rate k has a negative correlation with the initial concentration of phytic acid and has a positive correlation with temperature. As far as the dynamic absorption and desorption of phytic acid on D201 resin was concerned, the effects of chromatography column diameter, sample concentration, sample flowing velocity and eluant flowing velocity were notably observed.

  8. Comportamento do ácido fítico na presença de Fe(II e Fe(III Behaviour of phytic acid in the presence of iron(II and iron(III

    Directory of Open Access Journals (Sweden)

    Hanna Raquel Quirrenbach

    2009-03-01

    Full Text Available O ácido fítico, dependendo do valor de pH, apresenta alto potencial quelante, complexando íons metálicos, inibindo, assim, a produção de espécies reativas de oxigênio, responsáveis pela destruição oxidativa em sistemas biológicos. Esse potencial quelante tem fundamentado diversos estudos aplicados à ação antioxidante em produtos alimentícios. O objetivo deste trabalho foi estudar o grau de interação do ácido fítico com os íons metálicos Fe(II e Fe(III, de importância biológica, em condições próximas às fisiológicas, e a estabilidade destes complexos. Titulações potenciométricas foram conduzidas para determinar as constantes de formação dos complexos ácido fítico-Fe(II e ácido fítico-Fe(III em solução aquosa, atmosfera inerte, força iônica 0,100 mol.L-1 de cloreto de potássio e à temperatura de 36 ± 0,1 ºC. Estudos espectroscópicos na região do UV-Vis foram realizados para acompanhar a formação dos complexos em solução. Os complexos ácido fítico-Fe(II e ácido fítico-Fe(III foram sintetizados e caracterizados por espectroscopia de absorção na região do infravermelho e por estudos termoanalíticos. Os resultados do conjunto de técnicas utilizadas evidenciaram, tanto em solução como no estado sólido, a presença dos íons metálicos coordenados ao ácido fítico.Depending on the pH value, phytic acid presents high chelating potential bonding metallic ions thus inhibiting the production of reactivated oxygen species, which are responsible for the oxidative destruction in biological systems. Such chelating potential has been the basis of several studies on the antioxidant action in food products. The objective of this work was to study the degree of interaction of the phytic acid with the metallic ions Fe(II and Fe(III, of biological importance, in near-physiological conditions and the stability of these complexes. Potentiometric titrations were applied to determine the constants of

  9. Dietary phytic acid modulates characteristics of the colonic luminal environment and reduces serum levels of proinflammatory cytokines in rats fed a high-fat diet.

    Science.gov (United States)

    Okazaki, Yukako; Katayama, Tetsuyuki

    2014-12-01

    Dietary phytic acid (PA; myo-inositol [MI] hexaphosphate) is known to inhibit colon carcinogenesis in rodents. Dietary fiber, which is a negative risk factor of colon cancer, improves characteristics of the colonic environment, such as the content of organic acids and microflora. We hypothesized that dietary PA would improve the colonic luminal environment in rats fed a high-fat diet. To test this hypothesis, rats were fed diets containing 30% beef tallow with 2.04% sodium PA, 0.4% MI, or 1.02% sodium PA + 0.2% MI for 3 weeks. Compared with the control diet, the sodium PA diet up-regulated cecal organic acids, including acetate, propionate, and n-butyrate; this effect was especially prominent for cecal butyrate. The sodium PA + MI diet also significantly increased cecal butyrate, although this effect was less pronounced when compared with the sodium PA diet. The cecal ratio of Lactobacillales, cecal and fecal mucins (an index of intestinal barrier function), and fecal β-glucosidase activity were higher in rats fed the sodium PA diet than in those fed the control diet. The sodium PA, MI, and sodium PA + MI diets decreased levels of serum tumor necrosis factor α, which is a proinflammatory cytokine. Another proinflammatory cytokine, serum interleukin-6, was also down-regulated by the sodium PA and sodium PA + MI diets. These data showed that PA may improve the composition of cecal organic acids, microflora, and mucins, and it may decrease the levels of serum proinflammatory cytokines in rats fed a high-fat, mineral-sufficient diet.

  10. Phytic Acid Protects against 6-Hydroxydopamine-Induced Dopaminergic Neuron Apoptosis in Normal and Iron Excess Conditions in a Cell Culture Model

    Directory of Open Access Journals (Sweden)

    Qi Xu

    2011-01-01

    Full Text Available Iron may play an important role in Parkinson's disease (PD since it can induce oxidative stress-dependent neurodegeneration. The objective of this study was to determine whether the iron chelator, phytic acid (IP6 can protect against 6-hydroxydopamine- (6-OHDA- induced apoptosis in immortalized rat mesencephalic dopaminergic cells under normal and iron-excess conditions. Caspase-3 activity was increased about 6-fold after 6-OHDA treatment (compared to control; <.001 and 30 μmol/L IP6 pretreatment decreased it by 38% (<.05. Similarly, a 63% protection (<.001 against 6-OHDA induced DNA fragmentation was observed with IP6 pretreatment. Under iron-excess condition, a 6-fold increase in caspase-3 activity (<.001 and a 42% increase in DNA fragmentation (<.05 with 6-OHDA treatment were decreased by 41% (<.01 and 27% (<.05, respectively, with 30 μmol/L IP6. Together, our data suggest that IP6 protects against 6-OHDA-induced cell apoptosis in both normal and iron-excess conditions, and IP6 may offer neuroprotection in PD.

  11. Phytic acid attenuates inflammatory responses and the levels of NF-κB and p-ERK in MPTP-induced Parkinson's disease model of mice.

    Science.gov (United States)

    Lv, Yuqiang; Zhang, Zheng; Hou, Lin; Zhang, Li; Zhang, Jinyu; Wang, Yuehua; Liu, Cun; Xu, Pingping; Liu, Lu; Gai, Xiaoying; Lu, Tingxiu

    2015-06-15

    Phytic acid (PA) is a naturally occurring constituent which exhibits protective action in Parkinson's disease (PD). Inflammation in the central nervous system (CNS) is strongly associated with neuronal death in PD. However, the molecular mechanism of the protective effect of PA in PD has not been fully elucidated. In this study, we tried to testify the protection of PA on neuron and inflammatory responses in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD model of mice and investigated the mechanism involved in them. Motor behavior test and tyrosine hydroxylase (TH) immunohistochemistry method showed PA significantly inhibited MPTP-induced dopaminergic cell loss in the substantia nigra (SN). Moreover, using immunohistochemistry method and quantitative polymerase chain reaction (qPCR), microglial activation and inducible nitric oxide synthase (iNOS) were found to be markedly repressed by PA. Via western blot assay, expressions of nuclear factor κB (NF-κB) and phosphorylated extracellular signal-regulated kinase (p-ERK) were significantly attenuated by PA. In conclusion, it is suggested that PA has a neuroprotective effect in MPTP-induced PD model and the neuroprotection is correlated with its anti-inflammatory effect which may be associated with suppression of pathways that involved in NF-κB and p-ERK.

  12. Corrosion-controlling and osteo-compatible Mg ion-integrated phytic acid (Mg-PA) coating on magnesium substrate for biodegradable implants application.

    Science.gov (United States)

    Chen, Yingqi; Zhao, Sheng; Liu, Bo; Chen, Meiyun; Mao, Jinlong; He, Hairuo; Zhao, Yuancong; Huang, Nan; Wan, Guojiang

    2014-11-26

    Biodegradable, a new revolutionary concept, is shaping the future design of biomedical implants that need to serve only as a temporary scaffold. Magnesium appears to be the most promising biodegradable metal, but challenges remain in its corrosion-controlling and uncertain biocompatibility. In this work, we employ chemical conversion and alternating dip-coating methods to anchor and deposit an Mg ion-integrated phytic acid (Mg-PA) coating on Mg, which is supposed to function both corrosion-controlling and osteo-compatible. It was ascertained that PA molecules were covalently immobilized on a chemically converted Mg(OH)2 base layer, and more PA molecules were deposited subsequently via chelating reactions with the help of additive Mg ions. The covalent immobilization and the Mg ion-supported chelating deposition contribute to a dense and homogeneous protective Mg-PA coating, which guarantees an improved corrosion resistance as well as a reduced degradation rate. Moreover, the Mg-PA coating performed osteo-compatible to promote not only bioactivity of bonelike apatite precipitation, but also induced osteoblast cells adhesion and proliferation. This is ascribed to its nature of PA molecule and the biocompatible Mg ion, both of which mimic partly the compositional structure of bone. Our magnesium ion-integrated PA-coated Mg might bode well for the future of biodegradable bone implant application.

  13. Formation of Microcracks During Micro-Arc Oxidation in a Phytic Acid-Containing Solution on Two-Phase AZ91HP

    Science.gov (United States)

    Zhang, R. F.; Chang, W. H.; Jiang, L. F.; Qu, B.; Zhang, S. F.; Qiao, L. P.; Xiang, J. H.

    2016-04-01

    Micro-arc oxidation (MAO) is an effective method to produce ceramic coatings on magnesium alloys and can considerably improve their corrosion resistance. The coating properties are closely related with microcracks, which are always inevitably developed on the coating surface. In order to find out the formation and development regularity of microcracks, anodic coatings developed on two-phase AZ91HP after different anodizing times were fabricated in a solution containing environmentally friendly organic electrolyte phytic acid. The results show that anodic film is initially developed on the α phase. At 50 s, anodic coatings begin to develop on the β phase, evidencing the formation of a rough area. Due to the coating successive development, the microcracks initially appear at the boundary between the initially formed coating on the α phase and the subsequently developed coating on the β phase. With the prolonging treatment time, the microcracks near the β phase become evident. After treating for 3 min, the originally rough area on the β phase disappears and the coatings become almost uniform with microcracks randomly distributed on the sample surface. Inorganic phosphates are found in MAO coatings, suggesting that phytate salts are decomposed due to the high instantaneous temperature on the sample surface resulted from spark discharge.

  14. Synergistic bactericidal action of phytic acid and sodium chloride against Escherichia coli O157:H7 cells protected by a biofilm.

    Science.gov (United States)

    Kim, Nam Hee; Rhee, Min Suk

    2016-06-16

    The food industry must prevent the build-up of strong Escherichia coli O157:H7 biofilms in food processing environments. The present study examined the bactericidal action of phytic acid (PA), a natural extract from rice bran and the hulls/peels of legumes, against E. coli O157:H7 biofilms. The synergistic bactericidal effects of PA plus sodium chloride (NaCl) were also examined. E. coli O157:H7 biofilms were allowed for form on stainless steel coupons by culture in both rich (tryptic soy broth, TSB) and minimal (M9) medium at 22°C for 6days. Bacterial cells within biofilms grown in M9 medium were significantly more resistant to PA than those grown in TSB (pbiofilm effect of PA was significantly increased by addition of NaCl (2-4%) (pbiofilms without recovery (a>6.5logCFU/cm(2) reduction). Neither PA nor NaCl alone were this effective (PA, 1.6-2.7logCFU/cm(2) reduction; NaCl, food safety managers who encounter thick biofilm formation in food processing environments.

  15. 以米糠提取液为主料的气相缓蚀剂的缓蚀性能%Vapor Phase Inhibition Performance and Inhibition Mechanism of Phytic Acid Extracted from Rice Bran

    Institute of Scientific and Technical Information of China (English)

    康笑阳; 付朝阳; 胡胜; 钟飞

    2009-01-01

    Phytic acid was extracted from rice bran by leaching in a dilute acid.The resulting phytic acid was used as a main component to formulate a vapor phase inhibitor.The inhibition performance of the inhibitor for carbon steel in 3 % brine was investigated by conducting weight loss test and measurement of polarization curves and electrochemical impedance spectra.The inhibition mechanism of the inhibitor was also discussed.Results show that the corrosion inhibitor has good inhibition action for carbon steel subjected to vapor phase corrosion,and an inhibition efficiency of as much as 97% is obtained.As a kind of anodic adsorption inhibitor,the vapor phase inhibitor based on phytic acid is able to prohibit anode process and obeys the Langmuir's adsorption equation.%开发环境友好型缓蚀剂是缓蚀剂技术发展的必然趋势,从天然物中提取有效成分作为缓蚀剂就是途径之一,采用气相缓蚀剂可以有效减轻腐蚀,适应可持续发展的要求.用酸化浸取法从米糠中提取植酸,复配成了气相缓蚀剂;采用失重、极化曲线和阻抗测试等评价了该缓蚀剂的气相缓蚀效率,并对其缓蚀机理进行了初步探讨.结果表明:该缓蚀剂对碳钢的气相腐蚀有良好的缓蚀效果,缓蚀率可达97%;该缓蚀剂为阳极吸附型缓蚀剂,符合Langmuir吸附等温式.

  16. Effects of Phytic Acid Ketone on the Cell Proliferation of HeLa and Caski%植酸酮对人宫颈癌细胞增殖的影响

    Institute of Scientific and Technical Information of China (English)

    高晓丽; 王侃; 田文艳; 岳天孚

    2016-01-01

    Objective:To investigate the proliferation inhibitory effects of different density Phytic Acid Ketone on cervical cancer cell HeLa and Caski. Methods:Use the culture medium to dilute the type II No.1 and No.2 Phytic Acid Ketone to different density. The HeLa (adeno carcinoma, HPV 18 genome integrated) and Caski (squamous carcinoma, HPV 16 genome integrated) cells were treated with different concentration and time of Mefordcare. The inhibitory effects were evaluated with MTT assay. Results:The growth of HeLa and Caski was inhibited by different concentration of Mefordcare Phytic Acid Ketone, which was concentration-time dependent. The inhibitory effect on HeLa and Caski of Phytic Acid Ketone No. 1 and No. 2 varied with the concentration used, and the inhibitory rate came to the peak when concentration was about 5 860 mg/L and 5 500 mg/L. The inhibitory rate of cell proliferation was significantly different with the treating time at the same concentration (P<0.05). Conclusions:There were inhibitory effects from Phytic Acid Ketone on the growth of cervical cancer cell line HeLa and Caski. The result provides theoretical basis for the clinical application of Mefordcare Phytic Acid Ketone.%目的:研究不同浓度植物提取物植酸酮在不同作用时间对两种宫颈癌细胞系增殖的影响。方法:以培养基稀释植酸酮妇科清洗装置Ⅱ型1号及2号至相应浓度,采用噻唑蓝(MTT)比色法检测不同药物浓度对宫颈癌HeLa细胞[腺癌,含人乳头状瘤病毒18(HPV 18)基因]及Caski细胞(鳞癌,含HPV 16基因)不同时间点的增殖抑制率。结果:植酸酮妇科清洗装置Ⅱ型1号及2号试剂对HeLa及Caski细胞增殖的抑制作用具有浓度依赖性和时间依赖性。不同浓度植酸酮妇科清洗装置Ⅱ型1号及2号试剂对HeLa及Caski细胞增殖抑制作用差异有统计学意义(P<0.05),细胞增殖抑制率随给药浓度增加作用增强,浓度为5860 mg/L、5500 mg/L

  17. Characterization and corrosion behavior of phytic acid coatings, obtained by chemical conversion on magnesium substrates in physiological solution; Caracterizacion y comportamiento frente a la corrosion de recubrimientos de acido fitico, obtenidos por conversion quimica, sobre substratos de magnesio en solucion fisiologica

    Energy Technology Data Exchange (ETDEWEB)

    Hernandez-Alvarado, L. A.; Lomeli, M. A.; Hernandez, L. S.; Miranda, J. M.; Narvaez, L.; Diaz, I.; Garcia-Alonso, M. C.; Escudero, M. L.

    2014-10-01

    In order to improve the corrosion resistance of biodegradable magnesium and AZ31 magnesium alloy implants, a phytic acid coating has been applied on both substrates and their protective effect against corrosion has been assessed. The morphology and the chemical nature of the conversion coating were analyzed by SEM/EDX, XRD and FTIR. The spectra showed that the conversion coating was amorphous, and it was composed of Mg, O, and P on magnesium surface, along with Al, Zn and C on AZ31 alloy. The main coating components were chelate compounds formed by phytic acid and metallic ions. The corrosion resistance of bare and coated samples was evaluated by potentiodynamic polarization technique in Hank's solution at 37 degree centigrade. The results indicate that phytic acid conversion coatings provided a very effective protection to the magnesium substrates studied. (Author)

  18. Antioxidant Activities of Phytic Acid Crude Extracts from Rapeseed Meal in Vitro%菜籽饼中植酸粗提物的体外抗氧化能力研究

    Institute of Scientific and Technical Information of China (English)

    汤务霞; 祝佳; 熊治渝; 刘超

    2011-01-01

    [目的]研究三种不同溶剂体系从菜籽饼中提取得到的植酸粗提物的体外抗氧化性能力.[方法]通过Fenton 反应体系产生羟自由基(·OH),由连苯三酚在碱性溶液中自氧化反应产生超氧阴离子自由基(O2-·);用分光光度法检测三种植酸粗提物的还原能力及对·OH、O2-·和DPPH·的清除作用.[结果]三种溶剂提取得到的植酸粗提物都具有还原能力;对羟自由基、超氧阴离子自由基、DPPH自由基也用一定的清除能力.%[ObjectiveJThe aim was to study on antioxidant activities of phytic acid crude extracts with three solvent extracts from rapeseed meal in vitro. [MethodJThe hydroxyl radical ('OH)was produced through Fenton reaction system, and the superoxide anion radical (02-*) was produced from self-oixdation reaction in alkalescent solution with the help of pyrogallol; reduction ability of phytic acid crude extract and elimination effect on -OH^02'' and DPPH-were measured with spectrophotometry. [Results]Phytic acid of crude extracts with three solvent extracts had reduction ability and some elimination effect on hydroxyl radical, superoxide anion radical, DPPH radical.

  19. 小麦植酸含量的杂种优势和遗传力分析%Analysis of Heterosis and Hereditary on the Phytic Acid Content in Wheat

    Institute of Scientific and Technical Information of China (English)

    时侠清; 袁方方; 舒英杰

    2011-01-01

    Heterosis and Hereditary of Phytic acid in wheat were studied with 12 crosses from 4 parents by 4 × 4NC Ⅱ complete diallel crossing design. The result showed that over mid - parent heterosis and over low - parent heterosis of Yanzhan4110 × Huaimai20 in F1 is the strongest, over mid - parent heterosis of Yanzhan4110 × CN8081 in F1 is the least, Over low -parent heterosis of CN8081 x Xiaoyan986 in F1 is the least;the general combining ability value of Huaimai20 is the biggest and specific combining ability of Xiaoyan986 is the smallest; Broad heritability of phytic acid was 85.9% ,while narrow heritability of phytic acid was 32.3%.%选用4个材料亲本,按4×4NCⅡ完全双列杂交设计,配制了12个组合,对小麦植酸含量的杂种优势及其遗传力进行了研究。结果表明,偃展4110X淮麦20的中亲优势和超低亲优势最大,偃展4110×CN8081的中亲优势最小,CN8081×小偃986的超低亲优势最小;淮麦20的一般配合力效应值最大,小偃986的特殊配合力效应值最小;植酸的广义遗传力为85.9%,狭义遗传力为32.3%。

  20. 发芽对蚕豆中植酸和不同价态铁含量的影响%The Effect of Germination on Phytic Acid and Iron Content of Different Valence State in Faba Bean (Vicia faba L. )

    Institute of Scientific and Technical Information of China (English)

    罗羽洧; 王丽君; 郁志芸

    2012-01-01

    以蚕豆为研究对象,探讨了发芽过程中植酸和铁在蚕豆不同部位含量分布及变化情况。植酸随发芽时间延长含量在不断减少,发芽至第8天,子叶中植酸含量减少50%。在发芽过程中,铁含量在子叶中最高,其次是胚轴,而胚根和胚芽中含量最低。蚕豆发芽过程中铁的化学价态发生改变,其中有利于人体吸收的二价铁的比例显著增加。因此,通过发芽处理可提高蚕豆中铁的生物有效性。%Phytic acid and iron content in faba bean fractions during germination are investigat ed. Germination is an effective approach to reducing phytic acid in cotyledon and phytic acid is removed by 50% in cotyledon of the bean on the eighth day during germination. The cotyledon has the highest content of Fe, followed by embryonic axis, radicle and plumula. During germi nation, some mechanisms of faba bean can lead to the changing of iron chemical forms. By this mechanism the content of soluble ferrous iron increases obviously. So germination is a good way to improve the iron bioactivity in faba bean.

  1. A defective ABC transporter of the MRP family, responsible for the bean lpa1 mutation, affects the regulation of the phytic acid pathway, reduces seed myo-inositol and alters ABA sensitivity.

    Science.gov (United States)

    Panzeri, Dario; Cassani, Elena; Doria, Enrico; Tagliabue, Giovanni; Forti, Luca; Campion, Bruno; Bollini, Roberto; Brearley, Charles A; Pilu, Roberto; Nielsen, Erik; Sparvoli, Francesca

    2011-07-01

    • We previously identified the lpa1 (low phytic acid) 280-10 line that carries a mutation conferring a 90% reduction in phytic acid (InsP(6) ) content. In contrast to other lpa mutants, lpa1(280-10) does not display negative pleiotropic effects. In the present paper, we have identified the mutated gene and analysed its impact on the phytic acid pathway. • Here, we mapped the lpa1(280-10) mutation by bulk analysis on a segregating F(2) population, an then, by comparison with the soybean genome, we identified and sequenced a candidate gene. The InsP(6) pathway was analysed by gene expression and quantification of metabolites. • The mutated Pvmrp1(280-10) cosegregates with the lpa1(280-10) mutation, and the expression level of several genes of the InsP(6) pathway are reduced in the lpa1(280-10) mutant as well as the inositol and raffinosaccharide content. PvMrp2, a very similar paralogue of PvMrp1 was also mapped and sequenced. • The lpa1 mutation in beans is likely the result of a defective Mrp1 gene (orthologous to the lpa genes AtMRP5 and ZmMRP4), while its Mrp2 paralog is not able to complement the mutant phenotype in the seed. This mutation appears to down-regulate the InsP(6) pathway at the transcriptional level, as well as altering inositol-related metabolism and affecting ABA sensitivity.

  2. Use of phytic acid and hyper-salting to eliminate Escherichia coli O157:H7 from napa cabbage for kimchi production in a commercial plant.

    Science.gov (United States)

    Kim, Nam Hee; Jang, Seong Ho; Kim, Soon Han; Lee, Hee Jung; Kim, Younghoon; Ryu, Jee Hoon; Rhee, Min Suk

    2015-12-02

    The aim of this study was to develop a new salting method using natural phytic acid (PA) to ensure the microbiological safety and quality of salted napa cabbage used for kimchi production. The production of salted napa cabbage involves several stages: trimming, hyper-salting (20% NaCl) for up to 1h, salting (10% NaCl for 10-18 h), three sequential washes in water (30s for each), and draining (2 h). Two separate experiments were performed: one to determine the appropriate treatment conditions and a second to validate applicability under commercial conditions. In Experiment I, the effects of hyper-salting with PA on Escherichia coli O157:H7 numbers were tested in the laboratory. The following variables were monitored: 1) PA concentration (1, 2, 3%, w/w); 2) the ratio of the sample weight to the total volume of the solution (1:1.5, 1:3, or 1:6); 3) the hyper-salting time (30 or 60 min); and 4) the salting time (2, 5, or 8 h). A procedure that achieved a >5-log reduction in the E. coli O157:H7 population was then tested in an actual kimchi processing plant (Experiment II). The results from Experiment I showed that bactericidal efficacy increased as all the measured variables increased (p5-log CFU/g reduction in the E. coli O157:H7 population. Further salting for 5h completely eliminated (2.4-, and >1.8-log CFU/g, respectively. The 5h salting step maintained the TC and FC at napa cabbages were within the ranges required for kimchi production (pH 5.1-5.3 and 1.5-2.0%, respectively). These results suggest that this novel method of salting food ensures microbiological safety and reduces the production time.

  3. Phytic acid down-regulates IL-8 secretion from colonic epithelial cells by influencing mitogen-activated protein kinase signaling pathway.

    Science.gov (United States)

    Wawszczyk, Joanna; Orchel, Arkadiusz; Kapral, Małgorzata; Hollek, Andrzej; Weglarz, Ludmiła

    2012-01-01

    Phytic acid (IP6) is an essential component of high fiber diet physiologically present in human large gut. It has been recognized to possess various significant health benefits effects including chemopreventive and have antineoplastic activity against various types of cancer. Moreover, its role in immune response through modulation of the secretion of proinflammatory cytokines and chemokines has been postulated. One of the signal transduction pathways involved in a variety of inflammatory responses is p38 mitogen-activated protein kinase (MAPK) pathway. The aim of this study was to examine effect of IP6 on human p38alpha MAP kinase activity and the expression of gene encoding p38 MAP kinase in unstimulated and IL-1beta-stimulated Caco-2 cells. Furthermore, the role of signaling pathways involving p38 MAP kinase in IP6-induced down-regulation of IL-8 secretion by unstimulated and IL-1beta-stimulated cells in the presence of p38 MAP kinase activator (anisomycin) and inhibitor (SB 203580) was evaluated. IP6 inhibited activity of recombinant p38 MAPK activity in dose-dependent manner. Treatment of cells with IP6 for 3 h resulted in decreased p38 MAP kinase expression in both unstimulated and stimulated with IL-1beta cells. The similar level of p38alpha mRNA was found in untreated and treated with IP6 cells after 6 and 12 h. Incubation of Caco-2 cells with anisomycin resulted in upregulation of IL-8 secretion and their pretreatment with anisomycin prior to IP6 addition showed down-regulation of IL-8 secretion compared to cells treated with anisomycin alone. The findings of this study show that p38 MAPK could be one of the molecular targets for IP6 in the intestinal epithelial cells and that IP6 inhibitory effect on IL-8 secretion by Caco-2 cells could be mediated by its inhibition of p38 activity.

  4. Evaluation of the expression of metalloproteinases 2 and 9 and their tissue inhibitors in colon cancer cells treated with phytic acid.

    Science.gov (United States)

    Kapral, Małgorzata; Wawszczyk, Joanna; Jurzak, Magdalena; Dymitruk, Dominika; Weglarz, Ludmiła

    2010-01-01

    Matrix metalloproteinases 2 (MMP-2) and 9 (MMP-9) belong to a zinc dependent family of enzymes that degrade components of extracellular matrix. One postulated mechanism by which inositol hexaphosphate (phytic acid, IP6), an ubiquitous plant component, prevents the activation of MMPs may be due to its ability to chelate minerals. The aim of the study was to evaluate the expression profile of MMP-2, MMP-9 and their tissue inhibitors TIMP-1 and TIMP-2 at the mRNA level in human colorectal cancer cell line Caco-2 treated with IP6. A kinetic study of MMP-2, MMP-9 and TIMP-1, TIMP-2 mRNAs was performed after cells treatment with 1; 2.5; 5 mM IP6 for 1, 6, 12 and 24 h. Quantification of genes expression was carried out using real time QRT-PCR technique. The gene encoding MMP-9 was neither constitutively expressed nor induced by IP6 in Caco-2 cells. IP6 at the concentration of 1 mM evoked increase in MMP-2 transcript level, however, its higher doses (2.5; 5 mM) caused a decrease in this gene expression at 1 h incubation. In 24 h lasting culture along with increasing IP6 concentration, the cells expressed lower and lower MMP-2 mRNA level. In response to 1 and 2.5 mM at 6 h, the cells demonstrated an increased transcriptional activity of the TIMP-2 gene which was accompanied by a decrease in TIMP-1 gene transcription. Treatment of cells with 2.5 mM IP6 at 12 h resulted in a strong increase in both TIMP-1 and TIMP-2 expression. The results of this study show that IP6 modulates MMP-2, TIMP-1 and TIMP-2 genes expression in colon cancer cells at the transcriptional level in a way dependent on its concentration and time of interaction.

  5. The effect of phytic acid on the expression of NF-kappaB, IL-6 and IL-8 in IL-1beta-stimulated human colonic epithelial cells.

    Science.gov (United States)

    Wawszczyk, Joanna; Kapral, Małgorzata; Hollek, Andrzej; Weglarz, Ludmiła

    2012-01-01

    Intestinal epithelial cells play an important role in the mucosal immune and inflammatory reactions via the expression and secretion of proinflammatory cytokines such as interleukin-6 (IL-6) and interleukin-8 (IL-8). The expression of both interleukins is regulated by nuclear factor KB (NF-kappaB). Phytic acid (IP6) is an essential component of high fiber diet. It is physiologically present in the human large gut at concentrations reaching 4 mM. It exhibits pleiotropic health beneficial effects including anti-oxidant and anti-tumor activities. Recent studies showed that IP6 can modulate immune functions of intestinal epithelium through regulation of proinflammatory cytokines secretion. The aim of this study was to analyze the effect of IP6 on the expression of IL-6 and IL-8 as well as p50 and p65 subunits of NF-kappaB and its inhibitor IkappaBalpha in Caco-2 cells stimulated with IL-1beta. A kinetic study of mRNAs expression in cells was performed after their treatment with 1 and 2.5 mM IP6 for 3, 6 and 12 h. Quantification of the genes expression was carried out using real time QRT-PCR technique. IP6 at all used concentrations had no influence on transcription of p65 gene and modulated expression of p50 and IkappaBalpha genes in Caco-2 cells. Treatment of cells with IP6 resulted in a marked decrease in both IL-6 (at 3 and 6 h) and IL-8 expression (3 h). The results of these studies suggest that IP6 may exert immunoregulatory effects on intestinal epithelium by influencing transcriptional activity of genes encoding p50 subunit of NF-kappaB, its inhibitor IkappaBalpha and proinflammatory cytokines IL-6 and IL-8.

  6. Effect of phytic acid from rice and corn on morphology, cell proliferation, apoptosis and cyclooxygenase-2 expression in swine jejunal explants

    Directory of Open Access Journals (Sweden)

    Elisângela Olegário da Silva

    2014-06-01

    Full Text Available Phytic acid (IP6 is a potent antioxidant present in several natural foods. Beneficial effects on colon cancer and inflammation have been associated to IP6 in several studies, however, scarce data about the effect on small intestine are available. The aim of the present study was to evaluate the effect of different doses of IP6 from rice and corn on intestinal morphology, cellular proliferation, apoptosis and cyclooxygenase-2 (Cox-2 expression using swine jejunal explants as experimental model. This report demonstrated that explants treated with 0.5 mM, 2.5 mM and 5 mM of IP6 from rice and 2.5 mM and 5 mM from corn showed higher villi height compared to control. Explants treated with 2.5 mM and 5 mM IP6 from rice exhibited a significant reduction on intestinal histological changes (villi atrophy and fusion, edema, lymphatic vessel dilation, loss of apical enterocytes, cell vacuolation, necrotic debris, morphology of enterocytes and microvilli and number of villi. The cellular proliferation decreased in the explants treated with the dosages of 2.5 mM and 5 mM from rice and a significant decrease in cell apoptosis was observed in the treatments with 2.5 mM IP6 from rice and 5 mM IP6 from corn compared to the control. The explants treated with 2.5 mM and 5 mM IP6 from rice and corn showed a significant reduction of the Cox-2 expression. Higher dosages of IP6 from rice and corn used in this experiment increased the viability and preservation of intestinal tissue as evidenced by morphological and immunohistochemical assays.

  7. Pork meat increases iron absorption from a 5-day fully controlled diet when compared to a vegetarian diet with similar vitamin C and phytic acid content.

    Science.gov (United States)

    Bach Kristensen, Mette; Hels, Ole; Morberg, Catrine; Marving, Jens; Bügel, Susanne; Tetens, Inge

    2005-07-01

    Meat increases absorption of non-haem iron in single-meal studies. The aim of the present study was to investigate, over a 5 d period, the potential increasing effect of consumption of pork meat in a whole diet on the fractional absorption of non-haem iron and the total absorption of iron, when compared to a vegetarian diet. A randomised cross-over design with 3 x 5 d whole-diet periods with diets containing Danish-produced meat, Polish-produced meat or a vegetarian diet was conducted. Nineteen healthy female subjects completed the study. All main meals in the meat diets contained 60 g of pork meat and all diets had high phytic acid content (1250 mumol/d). All main meals were extrinsically labelled with the radioactive isotope (59)Fe and absorption of iron was measured in a whole body counter. The non-haem iron absorption from the Danish meat diet was significantly higher compared to the vegetarian diet (P=0.031). The mean fractional absorption of non-haem iron was 7.9 (se1.1), 6.8 (se 1.0) and 5.3 (se 0.6) % for the Danish and Polish meat diets and vegetarian diet, respectively. Total absorption of iron was higher for both meat diets compared to the vegetarian diet (Danish meat diet: P=0.006, Polish meat diet: P=0.003). The absorption ratios of the present study were well in accordance with absorption ratios estimated using algorithms on iron bioavailability. Neither the meat diets nor the vegetarian diets fulfilled the estimated daily requirements of absorbed iron in spite of a meat intake of 180 g/d in the meat diets.

  8. Differential expression of structural genes for the late phase of phytic acid biosynthesis in developing seeds of wheat (Triticum aestivum L.).

    Science.gov (United States)

    Bhati, Kaushal Kumar; Aggarwal, Sipla; Sharma, Shivani; Mantri, Shrikant; Singh, Sudhir P; Bhalla, Sherry; Kaur, Jagdeep; Tiwari, Siddharth; Roy, Joy K; Tuli, Rakesh; Pandey, Ajay K

    2014-07-01

    In cereals, phytic acid (PA) or inositol hexakisphosphate (IP6) is a well-known phosphate storage compound as well as major chelator of important micronutrients (iron, zinc, calcium, etc.). Genes involved in the late phases of PA biosynthesis pathway are known in crops like maize, soybeans and barley but none have been reported from wheat. Our in silico analysis identified six wheat genes that might be involved in the biosynthesis of inositol phosphates. Four of the genes were inositol tetraphosphate kinases (TaITPK1, TaITPK2, TaITPK3, and TaITPK4), and the other two genes encode for inositol triphosphate kinase (TaIPK2) and inositol pentakisphosphate kinase (TaIPK1). Additionally, we identified a homolog of Zmlpa-1, an ABCC subclass multidrug resistance-associated transporter protein (TaMRP3) that is putatively involved in PA transport. Analyses of the mRNA expression levels of these seven genes showed that they are differentially expressed during seed development, and that some are preferentially expressed in aleurone tissue. These results suggest selective roles during PA biosynthesis, and that both lipid-independent and -dependent pathways are active in developing wheat grains. TaIPK1 and TaMRP3 were able to complement the yeast ScΔipk1 and ScΔycf1 mutants, respectively, providing evidence that the wheat genes have the expected biochemical functions. This is the first comprehensive study of the wheat genes involved in the late phase of PA biosynthesis. Knowledge generated from these studies could be utilized to develop strategies for generating low phyate wheat.

  9. Effects of Ascorbic Acid, Phytic Acid and Tannic Acid on Iron Bioavailability from Reconstituted Ferritin Measured by an In Vitro Digestion/Caco-2 Cell Model

    Science.gov (United States)

    The effects of ascorbic acid, phytate and tannic acid on Fe bioavailability from Fe supplied as ferritin was compared to FeSO4 using an in vitro digestion/Caco-2 cell model. Horse spleen ferritin (HSF) was chemically reconstituted into a plant-type ferritin (P-HSF). In the presence of ascorbic acid...

  10. Inhibition Effect of the Composite of Tween-40 and Phytic Acid on Carbon Steel%吐温-40与植酸复配对碳钢的缓蚀作用

    Institute of Scientific and Technical Information of China (English)

    王文峰; 丁芳君; 刘丹婷; 周晓荣; 李建芬

    2015-01-01

    目的:提高0.5 mol/L HCl中植酸对热轧碳钢( HRCS)的缓蚀性能。方法用失重法和电化学阻抗法测试植酸、吐温-40以及复配缓蚀剂的缓蚀效率,从热力学和动力学方面分析复配缓蚀剂的作用机制。结果失重法测试表明,植酸和吐温均对热轧碳钢有一定缓蚀作用。当293 K,植酸质量浓度为0.3 g/L时,缓蚀效率达到69.1%,在质量浓度为0.8 g/L时缓蚀效率下降为9.6%;吐温-40的缓蚀效率随着质量浓度的增大而增大,0.8 g/L时达到73.4%。在0.5 mol/L盐酸中加入0.05 g/L吐温-40后,复配缓蚀剂的缓蚀效率随着质量浓度的增加而增大,且优于植酸和吐温-40单独使用,表现出协同效应。电化学测试得到了相同的结论,表明复配缓蚀剂在碳钢表面产生自发的Langmuir吸附,使碳钢腐蚀反应的活化能增大,是熵减少的放热过程。结论吐温-40和植酸在盐酸中对热轧碳钢的腐蚀有缓蚀协同作用。%Objective To improve the corrosion inhibition performance of phytic acid for hot rolled carbon steel (HRCS) in 0. 5 mol/L HCl soltuion. Methods The corrosion inhibition efficiency of phytic acid, Tween-40, and their compound on carbon steel was measured by weight-loss method and electrochemical impedance spectrometry tests. The inhibition mechanism of the compound inhibitor was analyzed using thermodynamics and kinetics. Results It was revealed by weight-loss measurement that either phytic acid or tween-40 alone had limited inhibitory effect on the corrosion of HRCS in hydrochloric acid. The inhibition efficiency of phy-tic acid reached the max value of 69. 1% when the concentration was 0. 3 g/L at 293 K, then decreased with further addition of in-hibitor and was reduced to 9. 6% when the concentration of phytic acid was 0. 8 g/L. On the other hand, the inhibition efficiency increased with the increase of the tween-40's concentration, which reached the value of 73. 4% at 0. 8 g/L, 293 K. When Tween-40 (0

  11. Effects of different nitrogen and water treatments on phytic acid contents and protein components in rice grain%氮素和水分处理对稻米植酸含量和蛋白组分的影响

    Institute of Scientific and Technical Information of China (English)

    张其芳; 刘奎刚; 苏达; 王复标; 程方民

    2012-01-01

    以直立密穗型粳稻品种"秀水110"和弯曲散穗型粳稻品种"春江15"为材料,对不同氮素水平处理和水分管理方式下稻米植酸含量和蛋白组分的影响及其互作效应进行了研究。结果表明,旱作栽培处理会导致水稻籽粒中植酸含量上升,而施氮处理对籽粒植酸含量的影响效应与水稻的水分管理方式有关,在常规水作条件下,高氮处理(N3)的籽粒植酸含量有所提高,但在旱作栽培方式下,中氮(N2)和低氮处理(N1,不施N)的籽粒植酸含量却略高于高氮处理(N3),氮素水平与水分管理方式间的互作效应明显;水稻籽粒植酸含量与粗蛋白总量、4种蛋白组分(清蛋白、球蛋白、醇溶蛋白和谷蛋白)及有关产量性状指标(有效穗数、每穗粒数、千粒重和结实率)在不同水肥处理间的相关性不显著,但氮肥用量过高不仅会导致水稻的产量水平下降,而且也不利于稻米营养品质的改良;水肥处理对水稻籽粒植酸含量、蛋白总量和4种蛋白组分穗内粒位分布也存在一定影响,着生在稻穗下部的弱势粒,其稻米植酸含量高于稻穗上部或中部的强势粒,因此改善弱势粒灌浆的水肥管理措施将有利于稻米植酸含量的降低。%Two rice genotypes (Xiushui 110 and Chunjiang 15 ) were used to analyze the influence of nitrogen treatments, water management methods and their interaction on grain phytic acid content and protein components by a field experiments of two water treatments, including traditional irrigated cultivation (C1) and dried stress cultivation (C2) with 3 level nitrogen fertilizers (N1, 0 kg/ha; N2, 100 kg/ha; N3, 200 kg/ha) for each water treatment. The results showed that C2 treatment (dried stress cultivation) had relatively higher phytic acid content in rice grain, while the effect of nitrogen treatments on grain phytic acid were water management-dependent, with slight

  12. 菜籽粕脱毒液中植酸的提取及其抗氧化活性%Extraction and Antioxidant Activity of Phytic Acid from Detoxified Rapeseed Meal Solution

    Institute of Scientific and Technical Information of China (English)

    兰文菊; 彭密军; 彭胜; 吕强

    2011-01-01

    Detoxified rapeseed meal solution was extracted by alkaline precipitation method to obtain phytic acid and the antioxidant activity of phytic acid obtained was evaluated through exploring its effect on acid value and peroxide value of lard using BHT as the positive control.Meanwhile,the antioxidant activity was also investigated through comparing its scavenging capacities against hydroxyl,superoxide anion and DPPH free radicals as well as hydrogen peroxide,determined by flow injection chemiluminescence and visible spectrophotometric methods,with those of VC.The results showed that the best condition for the extraction of phytic acid was pH 8.Under the optimal extraction condition,the yield of phytic acid was 92.83% and the purity was 45.96%.The extracted phytic acid could inhibit rancidity and peroxidation in lard with a better effect than that of BHT.The extract revealed the strongest scavenging effect against superoxide anion free radicals,followed by hydroxyl free radicals,hydrogen peroxide and DPPH free radicals.Moreover,it was superior to vitamin C in scavenging superoxide anion and hydroxyl free radicals.Therefore,the extract is a good natural antioxidant.%采用碱沉淀法从菜籽粕脱毒液中回收提取植酸并对该制备植酸的抗氧化活性进行研究。以BHT为阳性对照,考察植酸对猪油的酸价和过氧化值的影响;采用流动化学发光法和可见分光光度法,以VC为阳性对照,考察植酸清除羟自由基(.OH)、超氧阴离子自由基(O2.)、1,1-二苯基-2-苦苯肼自由基(DPPH自由基)及H2O2的能力。结果表明:回收提取植酸以NaOH调节溶液pH8为优,得率达92.83%,植酸含量为45.96%;植酸对猪油的酸败和过氧化有一定的抑制作用且优于BHT;制备植酸(实验制备)清除各种自由基的能力依次为:O2.〉.OH〉H2O2〉DPPH自由基,且除DPPH自由基外对其他自由基清除效果均优于VC。表明植酸是一种较好的天然抗氧化剂。

  13. Reasearch on Phytic Acid Passivation Technology of Stainless Steel and Corrosion Resistance%不锈钢植酸钝化工艺及其耐腐蚀性能研究

    Institute of Scientific and Technical Information of China (English)

    王海人; 石日华; 屈钧娥; 刘少波; 陈庚; 郭兴蓬

    2012-01-01

    通过极化曲线,AFM和红外光谱测试,研究了钝化工艺参数对不锈钢植酸钝化膜耐腐蚀性能的影响,得到了最优的钝化处理工艺,获得了耐腐蚀性能更优良的钝化膜.该方法原料来源广泛、价格低廉,工艺操作方便,而且钝化过程中不产生有毒有害物质,是一种高效环保型的不锈钢钝化处理方法.%The effect of process parameters of phytic acid passivation on the corrosion resistance performance of the stainless steel passivation film was studied by polarization curves, AFM and infrared spectrum testing. And the optimal process parameters were determined to obtain passivation film with excellent performance. For the phytic acid passivation method, the raw materials is easy to be obtained with low prices,and the operation is convenient. Moreover,passivation process does not produce poisonous and harmful substances,so it is a kind of high-efficiency environmental friendly passivation treatment method.

  14. 发酵法降解油菜籽废水中植酸的工艺研究%Degradation of Phytic Acid in Rapeseed Processing Water by Yeast Fermentation

    Institute of Scientific and Technical Information of China (English)

    黄耀武; 周建平

    2012-01-01

    水酶法提取菜籽油工艺产生了大量废水,其中含有丰富的水溶性蛋白、碳水化合物和抗营养因子.为了降解水中的抗营养物质植酸,利用筛选出的酵母菌对其进行发酵,并通过正交试验优化培养条件,得出K酵母在32℃,pH 7条件下培养36h,可将废水中植酸降解87%,从而为废水的循环利用或达标排放打下了基础.%There is plenty of process water after rapeseed oil extraction by aqueous enzymatic method. The process water contains water-soluble proteins, carbohydrates and anti nutritional factors. In order to depredate (he anti-nutrition-phytic acid, the yeast fermentation was conducted and the optimal culture condition was determined by orthogonal experiment. The result showed that phytic acid degradation rate could reach to 87% under optimal conditions which is cultural temperate at 32 °C, pH 7, and cultural time 36 h. The research provided references for rapeseed processing water recycling or discharging

  15. 放射型根瘤杆菌植酸酶对麸皮中植酸的脱磷作用%Dephosphorization of Phytic Acid in Wheat Bran by Phytase from Agrobacterium radiobacter

    Institute of Scientific and Technical Information of China (English)

    李文; 王陶; 杨克

    2011-01-01

    Crude phytase solution separated from Agrobacterium radiobacter fermentation broth was used to hydrolyze wheat bran for phytic acid dephosphorization.Using one-factor-at-a-time combined with orthogonal array design method,such process conditions as enzyme amount,substrate concentration,pH,temperature and hydrolysis time were optimized to be 750 U/g,40 mg/mL,7,45 ℃,and 5 h,respectively.Under the optimized conditions,the hydrolysis rate of phytic acid was up to 81.50%.%采用放射型根瘤杆菌植酸酶粗酶液水解麸皮中的植酸,研究其脱磷的最适条件。依据单因素试验和正交试验获得脱磷的最优条件为加酶量750U/g、底物质量浓度40mg/mL、温度45℃、pH7、时间5h。最优条件下,麸皮中植酸的水解率在5h达最大,为81.50%。

  16. Repeat length variation in the 5'UTR of myo-inositol monophosphatase gene is related to phytic acid content and contributes to drought tolerance in chickpea (Cicer arietinum L.).

    Science.gov (United States)

    Joshi-Saha, Archana; Reddy, Kandali S

    2015-09-01

    Myo-inositol metabolism plays a significant role in plant growth and development, and is also used as a precursor for many important metabolites, such as ascorbate, pinitol, and phytate. Phytate (inositol hexakisphosphate) is the major storage pool for phosphate in the seeds. It is utilized during seed germination and growth of the developing embryo. In addition, it is implicated in protection against oxidative stress. In the present study, a panel of chickpea accessions was used for an association analysis. Association analysis accounting for population structure and relative kinship identified alleles of a simple sequence repeat marker, NCPGR90, that are associated with both phytic acid content and drought tolerance. These alleles varied with respect to the dinucleotide CT repeats present within the marker. NCPGR90 located to the 5'UTR of chickpea myo-inositol monophosphatase gene (CaIMP) and showed transcript length variation in drought-tolerant and drought-susceptible accessions. CaIMP from a drought-tolerant accession with a smaller repeat was almost 2-fold upregulated as compared to a susceptible accession having a longer repeat, even under control non-stressed conditions. This study suggests an evolution of simple sequence repeat length variation in CaIMP, which might be regulating phytic acid levels to confer drought tolerance in natural populations of chickpea.

  17. Effect of Phytic Acid on Corrosion Resistance of 316L Stainless Steel in Salt Water%植酸对咸水介质中316L不锈钢耐蚀性的影响

    Institute of Scientific and Technical Information of China (English)

    张镇川; 陈友媛; 宋芳; 常钦鹏; 彭涛

    2016-01-01

    滨海区地源热泵中材料耐腐蚀性研究是有效开展利用新能源的基础,具有重要意义.采用动电位极化扫描和电化学阻抗谱方法研究了不同植酸组装浓度和组装时间对316L不锈钢在地下咸水介质中耐蚀性的影响,并探讨了植酸与钼酸钠复配形成的自组装膜对316L不锈钢在地下咸水介质中耐蚀性的影响.结果表明:在Cl-质量浓度为10 g/L的NaCl溶液中,随着植酸浓度的增加,316L不锈钢自腐蚀电流密度呈先减小后增大的趋势,自组装膜对316L不锈钢的防护作用先增强后降低;随着自组装时间的延长,植酸自组装膜对316L不锈钢的缓蚀率先上升后下降.在自组装时间为6 h、植酸浓度为10 mmol/L时形成的自组装膜防护效果最好,缓蚀率达到84.17%.向植酸自组装液中添加钼酸钠后,形成的聚合钼酸根通过络合作用和静电作用使316L不锈钢的耐蚀性降低,说明植酸的复配对提高咸水介质中316L不锈钢的耐蚀性是有选择的.这为咸水地区地源热泵系统中316L不锈钢换热器的防护提供了理论指导.%Corrosion resistance of metal materials in ground source heat pump is the basis study for effective u-tilization of new energy sources in the coastal zone,and has important significance.The effects of phytic acid concen-trations,self-assembled times and the addition of sodium molybdate on the corrosion behaviour of 316L stainless steel were investigated by potentiodynamic polarization curves and electrochemical impedance spectroscopy (EIS).The ex-perimental results showed that with the increase of the self-assembled time and the phytic acid concentration,the pro-tective effect was dropped after a rise in the NaCl solution which contains 10 g/L Cl-.The protective effect gained a maximum of about 84.17% while the self-assembled time and the phytic acid concentration were 6 h and 10 mmol/L, respectively.After adding sodium molybdate in the self-assembled solution

  18. Kinetic and equilibrium constants of phytic acid and ferric and ferrous phytate derived from nuclear magnetic resonance spectroscopy.

    Science.gov (United States)

    Heighton, Lynne; Schmidt, Walter F; Siefert, Ronald L

    2008-10-22

    Inositol phosphates are metabolically derived organic phosphates (P) that increasingly appear to be an important sink and source of P in the environment. Salts of myo-inositol hexakisdihydrogen phosphate (IHP) or more commonly phytate are the most common inositol phosphates in the environment. IHP resists acidic dephosphorylation and enzymatic dephosphorylation as ferric or ferrous IHP. Mobility of IHP iron complexes is potentially pH and redox responsive, making the time scale and environmental fate and transport of the P associated with the IHP of interest to the mass balance of phosphorus. Ferric and ferrous complexes of IHP were investigated by proton nuclear magnetic resonance spectroscopy ( (1)H NMR) and enzymatic dephosphorylation. Ferrous IHP was found to form quickly and persist for a longer period then ferric IHP. Dissociation constants derived from (1)H NMR experiments of chemically exchanging systems at equilibrium were 1.11 and 1.19 and formation constants were 0.90 and 0.84 for ferric and ferrous IHP, respectively. The recovery of P from enzymatic dephosphorylation of ferric and ferrous IHP was consistent with the magnitude of the kinetic and equilibrium rate constants.

  19. Corrosion Resistance of Phytic Acid Conversion Coating Modified by Molybdate on Surface of Aluminum Alloy%铝合金表面钼酸盐改进植酸转化膜的耐蚀性能

    Institute of Scientific and Technical Information of China (English)

    林碧兰

    2016-01-01

    目的:通过钼酸钠( SM)添加剂、SM前处理、SM后处理三种方案对铝合金表面植酸转化膜进行改进研究,以进一步提高其耐蚀性。方法通过动电位极化测试研究改进后铝合金在3.5%(质量分数) NaCl溶液中的耐蚀性。结果随着SM添加剂浓度的增加,铝合金表面植酸转化膜的耐蚀性先增强再减弱,SM质量浓度为30 g/L时的腐蚀保护效率P e 最大,达95.5%,而不含SM时的P e 仅为86.8%。 pH值太大(pH=8.0)或太小(pH=3.0)都不利于形成耐蚀性更好的膜层,pH值为6.0时的Pe 达98.6%。SM后处理会严重影响植酸转化膜的耐蚀性,腐蚀电流密度Jcorr大幅增大;SM前处理可提高植酸转化膜的耐蚀性,Pe达98.2%;SM前处理与添加剂同时应用时,植酸转化膜耐蚀性提高幅度更显著,Jcorr仅为0.042μA/cm2,极化电阻Rp达222 kΩ·cm2,Pe 达99.5%。结论 SM添加剂和SM前处理均可明显提高铝合金表面植酸转化膜的耐蚀性,且复合作用时的效果更显著,而SM后处理不能提高铝合金表面植酸转化膜的耐蚀性。%ABSTRACT:Objective To further enhance the corrosion resistance of phytic acid conversion coating on the surface of aluminum alloy, sodium molybdate ( SM) additives, SM pre-treatment and SM post-treatment were applied to modify the process. Methods The corrosion resistance of the modified aluminum alloy in 3. 5wt.% NaCl solution was investigated through dynamicpotential polar-ization test. Results With the increase in concentration of SM additives, the corrosion resistance of phytic acid conversion coating first increased and then decreased, and the corrosion protection efficiency Pe was the largest, reaching about 95. 5% when SM was 30 g/L, while the value was 86. 8% without SM additives. The Pe value reached 98. 6% at pH 6. 0, while a larger or a smaller pH (i. e. pH 8. 0 or pH 3. 0) was not beneficial to form a more anti-corrosion coating. However, the corrosion resistance of phytic acid

  20. 肌醇六磷酸对人骨肉瘤U2OS细胞的体外抑制作用%Anti-tumor effect of phytic acid on human osteosarcoma U2OS cells in vitro

    Institute of Scientific and Technical Information of China (English)

    王路; 程翠林; 赵海田; 崔洪斌

    2012-01-01

    Objective To explore the effect of phytic acid on the human osteosarcoma U2OS cells and its mechanisms in vitro. Methods MTT assay was used to examine the cell proliferation. Scanning electron microscope ( SEM) was used to observe the surface ultrastructure of U2OS cells. Hoechst 33342 fluorescence staining assay were applied to study the pro-apoptosis effects. The immune histochemisty method was used to detect c-myc expression. Results Phytic acid could significantly inhibit the growth of U2OS. The surface ultrastructure of U2OS cells showed distinct morphological changes corresponding to a typical cellular surface morphology of apoptosis. From fluorescence staining, we observed chromoplasm pyknosis and apoptotic body. Expression of c-myc protein decreased. Conclusion The anti-osteosarcoma mechanism of phytic acid may be related to its influence on inducing apoptosis and adjusting c-myc expression.%目的 探讨肌醇六磷酸体外对人骨肉瘤U2OS细胞的影响及其机制.方法 通过检测细胞增殖活性、超微扫描电镜观察、荧光染色法检测细胞凋亡及c-myc 蛋白表达来研究肌醇六磷酸对U2OS细胞的影响.结果 肌醇六磷酸能够抑制人骨肉瘤细胞U2OS的增殖,扫描电镜观察可见细胞表面超微结构发生凋亡改变,荧光显色证实肌醇六磷酸能够促细胞凋亡,c-myc蛋白的表达降低.结论 肌醇六磷酸在体外对人骨肉瘤U2OS细胞增殖的抑制机制可能与诱导细胞凋亡及调控c-myc蛋白表达相关.

  1. The variation of phytic and inorganic phosphorus in leaves and grain in maize populations

    Directory of Open Access Journals (Sweden)

    Dragićević Vesna

    2010-01-01

    Full Text Available The phytate function in plants is still not completely understood: it is the primary storage P form in seeds that is utilized during germination and early seedling development. Approaches to resolve problem of the bad nutritive quality of grain phytate include engineering of crops with reduced levels of seed phytic acid. The objective of this study was to investigate genetic variability and correlation of phytic (Pphy and inorganic phosphorus (Pi and soluble proteins among 28 maize populations, consisted into three groups: low-, intermediate- and high-phytic populations, with the aim to determine the potential of enhancing the P profile of maize plants and high grain yield through selection. The highest genetic variability of Pi and Pphy content in leaves was expressed in group with intermediate Pphy content in grain. Meanwhile, leaves of low-phytic populations were characterized with low Pphy, too (averagely 18% and high content of soluble proteins (averagely 15% in relation to high- and intermediate-phytic populations. Additionally, the lowest genetic variability of protein content was also noticeable in leaves of low-phytic populations. Positive correlation between Pi and protein content was observed in leaves of low- and high-phytic populations. The negative correlation between Pphy and Pi was detected in maize grain, but correlation was significant only in intermediate-phytic group. The highest, but not significant, average yield was observed in group of low-phytic populations, as well as its relative high genetic variability. That indicates that development of high yielding genotypes with lower phytate in grain is reasonable, and could be potentially useful in enhancing the sustainability and decreasing of environmental impact in agricultural production.

  2. 铁表面植酸钠自组装层缓蚀性能的电化学研究%Inhibition of iron surface with Na-salt of phytic acid self-assembled monolayers from corrosion:observed by electrochemistry

    Institute of Scientific and Technical Information of China (English)

    万琉方; 王芳; 郭玉辉; 陈慧文; 金溟南; 杨海峰

    2011-01-01

    将环境友好缓蚀剂植酸钠组装到铁基电极表面,运用电化学极化曲线方法,考察了不同组装液浓度、组装时间及次数形成的植酸钠层对铁基电极表面缓蚀性能的影响.实验结果表明:铁电极在10-2 mol·L-1和10-3 mol·L-1植酸钠溶液中分别组装数分钟,再经10-6mol·L-1植酸钠溶液组装7h,获得的植酸钠层对铁的缓蚀性能最佳.利用交流阻抗技术探究了最佳组装条件下形成的植酸钠层对铁电极表面的缓蚀机理,等效电路为R(QR)(QR).同时借鉴拉曼光谱进一步解释其缓蚀机理和组装方式.%Na-salt of phytic acid as an environmentally friendly corrosion inhibitor was self-assembled onto the surface of iron-based electrode. Effects of different assembly concentrations,including assembly time and assembly steps of Na-salt of phytic acid at iron-based electrode surface on anti-corrosion performance were evaluated by electrochemical polarization method. Experimental results revealed that the iron-based electrode with Na-salt of phytic acid monolayers formed by respective assemblies in 10- mol · L-1 and 10-3mol · L-1 Na-salt of phytic acid solutions for several minutes and then in l0-6mol · L-1 Na-salt of phytic acid solution for 7 hours exhibited the best anti-corrosive performance. AC impedance results demonstrated that the mechanism of Na-salt of phytic acid monolayers on iron-based electrode with the best anti-corrosive performance was consistent with the equivalent mode of R (QR) (QR) .which was further confirmed by Raman mapping.

  3. Increased dietary sodium chloride concentrations reduce endogenous amino acid flow and influence the physiological response to the ingestion of phytic acid by broiler chickens.

    Science.gov (United States)

    Cowieson, A J; Bedford, M R; Ravindran, V; Selle, P H

    2011-10-01

    A total of 240 Ross 308 broilers were used to investigate the effect of sodium (1·5 or 2·5 g/kg), phytate-P (0 or 3·2 g/kg), and phytase (0 or 1000 FTU/kg; 2x2x2 factorial) on endogenous amino acid flow using the enzyme-hydrolysed casein method. The ingestion of phytate increased endogenous amino acid flow (∼30%) compared with the phytate-free control diets. Phytase reduced endogenous amino acid flow only when fed in concert with phytate, resulting in a significant phytate x phytase interaction. Increasing dietary sodium concentration from 1·5 to 2·5 g/kg reduced endogenous amino acid flow by around 10%. This reduction of endogenous flow was particularly evident in diets which contained phytate, resulting in a significant sodium x phytate interaction for several amino acids, including Thr and Ser. Further, high sodium concentrations muted the effect of phytase resulting in a significant sodium x phytase interaction for some amino acids. The concentration of Asp, Thr, Ser and some other amino acids was increased in the endogenous protein in response to the ingestion of phytate. Both sodium and phytase essentially restored the composition of endogenous protein to that of the phytate-free control. Further, as both sodium and phytase had similar effects there were significant interactions between sodium and phytase for most amino acids, such that one was only effective in the absence of the other. These data confirm previous reports that phytate is a nutritional aggressor, causing quantitative and qualitative changes in endogenous protein flow. However, this is the first report which has shown that dietary sodium concentrations play a role in the severity of this antinutritional effect and consequently may blunt the efficacy of exogenous phytase. The mechanism is obscure, though it has been previously demonstrated that sodium can disrupt phytate:protein complexes, thus mitigating one of the mechanisms by which phytate exerts its antinutritional effect.

  4. Análise dos teores de ácidos cianídrico e fítico em suplemento alimentar: multimistura Analysis of hydrogen cyanide and phytic acid contents in feeding supplements: multimixture

    Directory of Open Access Journals (Sweden)

    Elizabete Helbig

    2008-06-01

    Full Text Available OBJETIVO: Este estudo objetivou quantificar o teor de ácido cianídrico em folhas de mandioca, que receberam tratamento prévio antes da secagem, e a concentração de ácido fítico na multimistura submetida à cocção úmida. MÉTODOS: Utilizou-se a multimistura produzida pela Pastoral da Criança da cidade de Pelotas (RS, constituída por: farelos de trigo (30% e arroz (30%: farinhas de milho (15% e trigo (10%; pós de casca de ovo (5%, de folha de mandioca (5% e de sementes (5%, abóbora ou girassol. Foi realizada orientação ao fornecedor da folha de mandioca sobre a forma recomendada de preparo antes da secagem. RESULTADOS: O conteúdo de ácidos cianídrico e fitatos no suplemento alimentar foram respectivamente de 85mg.kg-1 e 35.90mg.100-1. CONCLUSÃO: Verificou-se que a mudança na forma de secagem das folhas de mandioca foi eficiente para a redução de glicosídeos cianogênicos, e que o processo de torrefação dos ingredientes foi suficiente para produzir a redução de ácido fítico da multimistura aos níveis preconizados pela legislação, não sendo observadas diferenças estatisticamente significantes quando comparadas as amostras que também foram tratadas com calor úmido.OBJECTIVE: The objective of this study was to quantify the amount of hydrogen cyanide in cassava leaves that were treated before drying and the concentration of phytic acid in a multimixture submitted to wet cooking. METHODS: The multimixture produced by the Pastoral da Criança of the city of Pelotas (RS consisting of wheat flour (30%, rice flour (30%, corn flour (15%, wheat (10%, egg shell powder (5%, cassava leaves (5% and pumpkin or sunflower seeds (5% was used. The supplier was advised on how to process the cassava leaves before drying them. RESULTS: The hydrogen cyanide and phytic acid contents of the feeding supplement are 85mg.kg-1 and 35.90mg.100-1 respectively. CONCLUSION: Changing the way the cassava leaves were dried was efficient to reduce

  5. Experimental Study of Mefordcare Phytic Acid Ketone Effect on HeLa Cells Using MTT Assay%四甲基偶氮唑蓝法测定植酸酮对HeLa细胞的作用

    Institute of Scientific and Technical Information of China (English)

    武志丹; 杨志谋; 岳天孚

    2012-01-01

    目的:分析植酸酮妇科清洗装置(商品名:美福康)Ⅱ型l号及2号对体外培养的HeLa细胞生长的抑制作用,为后期实验提供实验依据.方法:体外培养HeLa细胞,分为实验组和对照组,实验组从8个浓度水平上给药,分别经过24,48,72h后用四甲基偶氮唑蓝(MTT)法检测药物对HeLa细胞增殖的影响.结果:与对照组相比,经2种型号的药物作用后的HeLa细胞存活率降低,凋亡增加,作用呈时间-剂量依赖关系.结论:植酸酮妇科清洗装置Ⅱ型1号及2号均能有效抑制宫颈癌HeLa细胞的生长.%Objective:To study the ability of the No.l and No.2 of type II of Mefordcare Phytic Acid Ketone on inhibiting the proliferation of HeLa cells which were cultivated in vitro,and provide the evidence for father experiment. Methods: The HeLa cells which were cultivated in vitro was treated by eight different concentrations for 24,48 and 72h,MTT assay was employed to examine the growth suppression of HeLa cells. Results:Contrast with the control group,the apoptosis of the cells treated with these two numbers of the drug was increased and the growth rate was decreased. And the inhibition of HeLa cells in partly is in a concentration-dependent and time-dependent manner. Conclusions:The No.l and No.2 of type II of Mefordcare Phytic Acid Ketone could significantly inhibit the proliferation of HeLa cells.

  6. 环境中植酸的分布、形态及界面反应行为%Distribution, Species and Interfacial Reactions of Phytic Acid in Environment

    Institute of Scientific and Technical Information of China (English)

    严玉鹏; 万彪; 刘凡; 谭文峰; 刘名茗; 冯雄汉

    2012-01-01

    肌-肌醇六磷酸(myo-inositol-1,2,3,4,5,6-hexakisphosphate,IHP,myo-IP6)简称植酸(Phytic acid或phytate),是大多数土壤中最为丰富的有机磷,它在环境中的界面反应影响着磷素的迁移、循环、转化、生物有效性以及环境效应.本文简要总结了环境中植酸的分布与形态,并综述了其界面反应,包括铁铝氧化物、粘土矿物、碳酸钙等对植酸的吸附解吸,多价阳离子与植酸的络合反应以及沉淀作用.土壤矿物对植酸的吸附影响其转化和生物有效性,矿物对植酸磷的吸附量一般远高于正磷酸盐.其中,弱晶质铁铝(氢)氧化物对植酸的吸附能力一般较晶形铁铝(氢)氧化物大很多.同时,分析了矿物类型、介质pH、温度、共存离子等环境条件对植酸界面反应的影响,植酸吸附对矿物表面电荷性质、颗粒大小和分散稳定性等的作用,以及植酸在金属污染土壤修复中的应用前景.最后讨论了环境中植酸的主要研究热点和方向.%myo-inositol-l,2,3,4,5,6-hexakisphosphate is called phytic acid or phytate, and commonly abbreviated as IHP or myo-lP6. IHP is the most abundant organic phosphorus in most soils. The mobility, cycling, transport, bioavailability to plants and environmental impact of soil phosphorus are affected by the interfacial reactions of IHP in the environment. This paper briefly summarized the distribution and species of phytic acid in the environment, and interfacial reactions, which including adsorption and desorption of IHP by soil components, such as iron and aluminum oxides, clays, calcium carbonate and so on, complexation reactions and precipitation with polyvalent cations. The transport and bioavailability of IHP are affected by its adsorption on soil minerals. The adsorption capacity of minerals for IHP is much greater than that for orthophosphate. The adsorption capacity of amorphous Fe/Al-(hydr)oxides for IHP is generally higher than that of crystalline Fe

  7. 60Co-γ辐照高产低植酸小麦新品系的鉴定筛选%Screening and Identification of New High-Yielding Wheat Lines with Low Phytic Acid Induced by 60Co-γRadiation

    Institute of Scientific and Technical Information of China (English)

    郎淑平; 马燕欣

    2016-01-01

    通过辐射诱变手段获得种子低植酸(low phytic acid)突变系,进而选育低植酸作物品系,是可行的技术路径。本研究利用60 Co-γ辐照小麦种子,从其后代中筛选到熟期与辐射亲本相仿或早熟、高产且籽粒植酸含量明显低于辐射亲本的小麦新品系10份,为小麦育种提供新的种质资源。%In recent years,it has emerged as a research hotspot that crop mutant lines with low phytic acid (LPA)are induced by radia-tion mutagenesis,which are subsequently used as materials to breed LPA varieties.In this study,by screening a large number of 60 Co-γradiated wheat seeds,we obtained 10 new wheat lines.Compared to their parental variety,the new wheat lines attained higher yield and had lower phytic acid content while having similar or earlier maturity time.These new wheat lines can be used as a new source of wheat germplasm for breeding LPA varieties in the future.

  8. QTL Mapping and Epistasis Analysis for Phytic Acid Concentration in Rice Grain by Using the Bayesian Model Selection%利用贝叶斯法进行水稻籽粒植酸含量性状的QTL定位及互作分析

    Institute of Scientific and Technical Information of China (English)

    李茂柏; 王慧; 张建明; 李丁鲁; 杨润清; 周宇琼; 朴钟泽

    2009-01-01

    Main QTL and epistasis interaction effects for phytic acid concentration in rice grain were investigated by using a F_2 population consisted of 172 lines derived from the cross between an indica rice LPA(the grain phytic acid concentration was 7.11 mg/g) and a japonica rice Zhonghua 11(the phytic acid concentration was 11.92 mg/g) with the Bayesian model selection. A genetic linkage map including 126 SSR and 4 STS markers was constructed with the F_2 population. Three main QTLs related to phytic acid concentration in rice grain were detected. They were located on chromosomes 3, 5, and 6, explaining 5.38%, 8.02%, and 4.62% of phenotypic variation, respectively. The three alleles for reducing the phytic aid concentration were from the parent LPA. Ten pairs of epistatic interaction were detected on chromosomes 1, 3, 5, 6, and 11, with the interaction effects from 1.69 to 5.18 and the percentages of phenotypic variance ranged from 8.67% to 24.73%.%利用水稻植酸含量差异较大的品种中花11(粳型)和LPA(籼型)为亲本杂交获得F_2群体的172个单株,构建了含126个SSR和4个STS标记的遗传连锁图谱,利用贝叶斯(Bayesian)法对水稻籽粒植酸含量性状进行了主效应QTL定位和上位性互作分析.共检测到 3 个与水稻籽粒植酸含量性状有关的主效QTL,分布在第3、5和6 染色体的相应区间内,表型贡献率分别为5.38%、8.02%和4.62%,降低籽粒植酸含量的等位基因均来自亲本LPA.检测到10对上位性互作影响籽粒植酸含量, 分布于水稻第1、3、5、6、11染色体上,互作效应值为1.69~5.18,其表型变异的解释率为8.67%~24.73%.

  9. Over-expression of the bacterial phytase US417 in Arabidopsis reduces the concentration of phytic acid and reveals its involvement in the regulation of sulfate and phosphate homeostasis and signaling.

    Science.gov (United States)

    Belgaroui, Nibras; Zaidi, Ikram; Farhat, Ameny; Chouayekh, Hichem; Bouain, Nadia; Chay, Sandrine; Curie, Catherine; Mari, Stéphane; Masmoudi, Khaled; Davidian, Jean-Claude; Berthomieu, Pierre; Rouached, Hatem; Hanin, Moez

    2014-11-01

    Phytic acid (PA) is the main phosphorus storage form in plant seeds. It is recognized as an anti-nutrient for humans and non-ruminant animals, as well as one of the major sources of phosphorus that contributes to eutrophication. Therefore, engineering plants with low PA content without affecting plant growth capacity has become a major focus in plant breeding. Nevertheless, lack of knowledge on the role of PA seed reserves in regulating plant growth and in maintaining ion homeostasis hinders such an agronomical application. In this context, we report here that the over-expression of the bacterial phytase PHY-US417 in Arabidopsis leads to a significant decrease in seed PA, without any effect on the seed germination potential. Interestingly, this over-expression also induced a higher remobilization of free iron during germination. Moreover, the PHY-over-expressor lines show an increase in inorganic phosphate and sulfate contents, and a higher biomass production after phosphate starvation. Finally, phosphate sensing was altered because of the changes in the expression of genes induced by phosphate starvation or involved in phosphate or sulfate transport. Together, these results show that the over-expression of PHY-US417 reduces PA concentration, and provide the first evidence for the involvement of PA in the regulation of sulfate and phosphate homeostasis and signaling.

  10. Effect of γ-amino Butyric Acid and Phytic Acid Content in Germinated Brown Rice by Adding Exogenous Phytase%外源植酸酶对发芽糙米γ一氨基丁酸和植酸含量的影响

    Institute of Scientific and Technical Information of China (English)

    刘玮; 曹龙奎

    2015-01-01

    Effects of adding exogenous phytase on germinated brown rice γ-amino butyric acid and phytic acid content were studied.γ-amino butyric acid content in germinated brown rice could be increase, and levels of phytase could be reduce by adding exogenous phytase, thus nutritional value of brown rice germination could be improve.%本文研究了添加外源植酸酶对发芽糙米γ一氨基丁酸和植酸含量的影响,通过添加外源植酸酶,可以增加发芽糙米γ一氨基丁酸含量,降低植酸酶含量,从而提高发芽糙米的营养价值。

  11. 不锈钢植酸转化处理对基材与水性耐指纹涂料结合性能的影响%Effect of Phytic Acid Conversion Treatment of Stainless Steel on Adhesion of Water - Borne Anti - Fingerprint Coating to Steel Substrate

    Institute of Scientific and Technical Information of China (English)

    王海人; 谢娥梅; 屈钧娥; 曹志勇; 李艳; 李敏娅

    2013-01-01

    430 stainless steel was treated with phytic acid so as to improve its binding strength with epoxy resin acrylate water-based anti-fingerprint paint modified by organosili-con. The chemical structure and water contact angle of resultant phytic acid conversion coating were analyzed with an infrared spectrometer and a contact angle meter. The corrosion resistance of the anti - fingerprint coating was evaluated in relation to mea -surement of the alternating current impedance spectrum and potentiodynamic polarization curve. It was found that the stainless steel treated by phytic acid had good hydrophilicity and adhesion to the water-borne paint, and as-prepared anti-fingerprint coating had good corrosion resistance.%不锈钢表面很容易钝化,水性涂料很难在其上均匀成膜.为此,采用植酸转化处理后有效桥接430不锈钢和有机硅改性环氧丙烯酸水性耐指纹涂料.通过红外光谱、接触角、交流阻抗测试和动电位扫描等考察了植酸转化膜及其涂覆水性涂料后的性能.结果显示,经植酸转化处理后,不锈钢基体与植酸发生了桥接,与水的接触角更小,具有很好的亲水性,与水性涂料结合良好,提高了不锈钢的耐蚀性能.

  12. Ácido fítico de híbridos de milho e alguns produtos industrializados = Phytic acid in corn hybrids and in some industrialized corn products

    Directory of Open Access Journals (Sweden)

    Tatiana Shizue Fukuji

    2008-01-01

    Full Text Available O ácido fítico (AF ou mio-inositol hexafosfato está presente principalmente em cereais, e o germe de milho apresenta teor elevado, com cerca de 6,0 a 7,0% em base seca. Devido a sua propriedade quelante com metais di e tri-valentes, o AF apresenta capacidade antioxidante com eficaz atuação na inibição de reações de oxidação. O teor de AF foi determinado no germe e endosperma de 11 híbridos de milhos, cultivados no Estado do Paraná, e em diferentes produtos industrializados de milho. Os germes de híbridos de milho foram caracterizados como componentes do milho, com elevado teor e osendospermas com baixo teor de AF. Os produtos derivados de milho, elaborados basicamente com endosperma tais como canjica, creme de milho, farinha de milho e fubá fino, apresentaram menor teor de AF, enquanto aqueles originários dos germes desengordurado, fino, gordo e película de milho apresentaram maior teor de AF.Phytic acid (PA, also known as myoinositol hexaphosphate, is found mainly in cereal grains. Corn germ has high concentrations of PA – from 6.0 to 7.0% on a dry weight basis. Due to its chelating properties on di- and trivalent metals, PA has antioxidantattributes, effectively inhibiting oxidation reactions. In this study, PA levels were determined in the germ and endosperm of eleven corn hybrids cultivate in Paraná State (Brazil, and also in several industrialized corn products. Corn hybrid germs were characterized as corn components with high PA levels, whereas endosperms featuredlow levels of PA. Corn-based industrialized products, derived mostly from corn endosperm (such as canjica, creamed corn, corn flour and cornmeal featured the lowest PA values. Conversely, defatted corn germs and corn cuticle showed the highest PA levels.

  13. InsP6-sensitive variants of the Gle1 mRNA export factor rescue growth and fertility defects of the ipk1 low-phytic-acid mutation in Arabidopsis.

    Science.gov (United States)

    Lee, Ho-Seok; Lee, Du-Hwa; Cho, Hui Kyung; Kim, Song Hee; Auh, Joong Hyuck; Pai, Hyun-Sook

    2015-02-01

    Myo-inositol-1,2,3,4,5,6-hexakisphosphate (InsP(6)), also known as phytic acid, accumulates in large quantities in plant seeds, serving as a phosphorus reservoir, but is an animal antinutrient and an important source of water pollution. Here, we report that Gle1 (GLFG lethal 1) in conjunction with InsP(6) functions as an activator of the ATPase/RNA helicase LOS4 (low expression of osmotically responsive genes 4), which is involved in mRNA export in plants, supporting the Gle1-InsP(6)-Dbp5 (LOS4 homolog) paradigm proposed in yeast. Interestingly, plant Gle1 proteins have modifications in several key residues of the InsP(6) binding pocket, which reduce the basicity of the surface charge. Arabidopsis thaliana Gle1 variants containing mutations that increase the basic charge of the InsP(6) binding surface show increased sensitivity to InsP(6) concentrations for the stimulation of LOS4 ATPase activity in vitro. Expression of the Gle1 variants with enhanced InsP(6) sensitivity rescues the mRNA export defect of the ipk1 (inositol 1,3,4,5,6-pentakisphosphate 2-kinase) InsP(6)-deficient mutant and, furthermore, significantly improves vegetative growth, seed yield, and seed performance of the mutant. These results suggest that Gle1 is an important factor responsible for mediating InsP(6) functions in plant growth and reproduction and that Gle1 variants with increased InsP(6) sensitivity may be useful for engineering high-yielding low-phytate crops.

  14. 壳聚糖植酸天然复合涂膜对鲜切莲藕保鲜效果的研究%Study of Natural Film with Chitosan Combining Phytic Acids on Preservation of Fresh-cutting Lotus Root

    Institute of Scientific and Technical Information of China (English)

    于有伟; 李惠; 邸金花; 任引哲

    2012-01-01

    Using chitosan, phytic acid and their combination to film fresh-cutting lotus root, the effect of different film on preservation was studied. Hie result showed that compound film with 1% chitosan and 1% phytic acid could an reduce the weight loss rate and MDA content of fresh-cutting lotus root, postpone browning, restrain the activities of POD, PPO and PAL, and keep the content of vitamin C and polyphenol at relatively high level. At the 8 th day, the weight loss rate of fresh-cutting lotus root with compound film was one half of control, MDA content was lower 18.8% and L value was higher 51.2% than control. Compound film with chitosan and phytic acid is a simple but effective method to preserve fresh-cutting lotus root, which is superior to single chitosan or phytic acid%采用1%壳聚糖、1%植酸和壳聚糖植酸复合涂膜处理鲜切莲藕,研究不同处理对鲜切莲藕贮存效果的影响.研究结果表明,复合涂膜处理能够降低鲜切莲藕的失重率和MDA含量,延缓藕片的褐变,抑制POD、PPO、PAL的活性,同时使维生素C和多酚含量维持在较高水平.贮存至第8天,复合涂膜的鲜切莲藕失重率为对照的1/2,MDA含量比对照低18.8%,L值比对照高51.2%.壳聚糖植酸复合涂膜是一种简便有效的贮存鲜切莲藕的方法,效果优于壳聚糖或植酸单一处理.

  15. 植酸在316L不锈钢表面的自组装及缓蚀性能研究%Research on Self-assembly Behavior and Corrosion Inhibition Performance of Phytic Acid on 316L Stainless Steel Surface

    Institute of Scientific and Technical Information of China (English)

    王海人; 周洋; 屈钧娥; 王海鹏; 曹志勇

    2013-01-01

    以浸泡法在化学氧化处理前后的316L不锈钢表面制备了植酸自组装膜,通过动电位扫描、SEM观察及FT-IR测试,研究了组装液pH与组装时间对植酸自组装膜吸附行为及耐蚀性能的影响.结果表明:植酸被成功地组装到316L不锈钢表面,并使其耐腐蚀性能大大提高.相比而言,化学氧化处理有利于形成更致密、更耐腐蚀的自组装膜.不经化学氧化处理时,植酸组装的最佳pH为6.90,最佳时间为12 h;而经化学氧化处理时,植酸组装的最佳pH也为6.90,但最佳时间缩短为4h.%The phytic acid self-assembled film (SAM) was prepared on 316L stainless steel surface before and after chemical oxidation by immersion method.The effect of SAM solution pH and SAM time on the adsorption behavior and corrosion inhibition performance of the phytic acid SAM was investigated by potential dynamic scan,SEM (scanning electron microscope) observation and FT-IR (Fourier transformed infrared spectroscopy) testing.The experimental results show that phytic acid has been successfully assembled to the surface of 316L stainless steel,which greatly improves the corrosion resistance of the metal.The chemical oxidation pretreatment is beneficial for the formation of a denser SAM with more notable corrosion resistant performance.For the samples without chemical oxidation treatment,the optimum pH for the assembly of phytic acid is 6.90 and the best assembly time is 12 h.And for those with chemical oxidation,the optimum pH is still 6.90 while the best assembly time reduces to 4 h.

  16. Adições crescentes de ácido fítico à dieta não interferiram na digestibilidade da caseína e no ganho de peso em ratos Increasing quantities of phytic acid in the diet did not affect casein digestibility and weight gain in rats

    Directory of Open Access Journals (Sweden)

    Admar Costa de Oliveira

    2003-06-01

    Full Text Available O crescente consumo de alimentos de origem vegetal, sejam como fontes protéicas com baixo teor de gordura ou como fontes de fibras, tem acrescido à dieta humana o ácido fítico. Devido à sua carga altamente negativa, o ácido fítico tem sido visto como componente de ação antinutricional capaz de quelar minerais bivalentes, proteínas e amido, podendo comprometer a biodisponibilidade destes nutrientes. No presente estudo investigou-se a influência da adição de ácido fítico à dieta de caseína, em concentrações iguais ou até oito vezes superiores àquelas encontradas no feijão-comum Phaseolus vulgaris, cultivar IAC-Carioca (14,7mg de ácido fítico/g feijão cru, durante período experimental de dez dias, sobre os índices nutricionais Ganho de Peso, Quociente de Eficiência da Dieta, Quociente de Eficiência Protéica Líquida, Digestibilidade Aparente e Digestibilidade Verdadeira. Trinta e seis ratos machos SPF da linhagem Wistar, recém-desmamados, divididos em grupos experimentais com seis ratos cada, foram alimentados com dieta purificada AIN-93G isenta de ácido fítico (Controle e dietas teste AIN-93G acrescidas de 218, 436, 872 e 1744mg de ácido fítico/kg de dieta (Tratamentos. Os ganhos de peso (g e os índices de qualidade dietética e protéica não apresentaram diferença estatística (p>0,05, e os valores médios entre os grupos foram: Ganho de Peso: 59,5 ± 5,0g; Quociente de Eficiência da Dieta: 0,39 ± 0,01; Quociente de Eficiência Protéica Líquida: 3,64 ± 0,12; Digestibilidade Aparente: 92,7 ± 1,1% e Digestibilidade Verdadeira: 94,4 ± 0,9%. Os resultados demonstraram que nas condições experimentais utilizadas, o ácido fítico não foi capaz de alterar o valor nutritivo da caseína.The recent increasing consumption of vegetal origin foods, in order to obtain protein or fiber sources with low fat, has led to the presence of various amounts of phytic acid in the diet. The phytic acid has a strong negative

  17. Phytic acid: How far have we come?

    African Journals Online (AJOL)

    STORAGESEVER

    2010-03-15

    Mar 15, 2010 ... African Journal of Biotechnology Vol. 9(11), pp. ... biotechnology and genetic manipulations. Now it ... 1993) and may function as a neurotransmitter (Vallejo et .... by pIAβ8 and pGAPZαA vectors and analysis of its biochemical.

  18. Impact of celecoxib on soluble intercellular adhesion molecule-1 and soluble e-cadherin concentrations in human colon cancer cell line cultures exposed to phytic acid and TNF-alpha.

    Science.gov (United States)

    Parfiniewicz, Beata; Pendzich, Joanna; Gruchlik, Arkadiusz; Hollek, Andrzej; Weglarz, Ludmiła

    2012-01-01

    Soluble adhesion molecules such as soluble intercellular adhesion molecules-1 (sICAM-1) and soluble E-cadherin (sE-cadherin) play important role in tumor invasion and the development of metastasis. It was observed that their concentrations in body fluids of patients with colon cancer were elevated. Celecoxib, a selective inhibitor of cyclooxygenase-2 (COX-2) besides its analgesic, anti-inflammatory, and antipyretic activity is able to inhibit development of colon cancer and reduce risk of metastasis. The additional factors, e.g., dietary components in colon cancer, may influence therapeutic effect of drugs, such as cytokines. TNF-alpha (tumor necrosis factor - alpha) is a cytokine, which concentration significantly increases in serum of patients with inflammatory and cancer diseases. The latest studies demonstrate, that phytic acid (IP6), a myo-inositol derivative, abundantly present in high-fiber diets could substantially reduce colon cancer incidence. The aim of the present study was to evaluate the influence of celecoxib on sICAM-1 and sE-cadherin concentrations in transformed epithelial colon cell cultures simultaneously exposed to IP6 and TNF-alpha. Additionally, the adhesion of the exposed cells to collagen I was assessed. HT-29 and Caco-2 cells were cultured in the presence of 50 ng/mL celecoxib, 1.0 mM IP6, and 100 ng/mL TNF-alpha, and their combination: TNF-alpha plus IP6, TNF-alpha plus celecoxib, IP6 plus celecoxib, and TNF-alpha with celecoxib plus IP6, for 96 h. Nonexposed cell line cultures served as controls. Concentrations of sICAM-1 and sE-cadherin were measured in the culture medium by enzyme-linked immunosorbent assay (ELISA) using Quantikine - Human sICAM-1/CD54 Immunoassay and Quantikine-Human sE-Cadherin Immunoassay. All the results obtained were expressed as ng per mL. In the adhesion assay, the cells were incubated with IP6 (0.5, 1.0 and 2.0 mM), TNF-alpha (100 ng/mL), celecoxib (50 ng/mL) and their combination for 90 min. Fluorescence

  19. The Effect of NaCl Stress on Seed Germination of Wheat Mutants with Low Phytic Acid%NaCl 胁迫对低植酸小麦突变体材料种子萌发特性的影响

    Institute of Scientific and Technical Information of China (English)

    郎淑平; 马燕欣

    2016-01-01

    Phytic acid (PA)is naturally stored in seeds of a wide range of cereal and legume crops.In recent years,it has become a research hotspot to breed crop varieties with low phytic acid (LPA)by using mutagenesis technology.In the current research,we ob-tained nine LPA wheat mutants by 60 Co -γradiation,and then assessed their salt tolerance using a germination test with different con-centrations of NaCl.As a result,their seed germination was promoted at 0.5% NaCl concentration,but substantially inhibited at 1.0% and 1.5% NaCl concentrations;all tested NaCl concentrations produced an inhibitory effect on seedling growth and primary root growth.Among the nine LPA wheat mutants,four (lp14,lp49,lp50,and lp55)showed higher salt tolerance,which may be used as materials for breeding wheat varieties with salt tolerance in the future.%植酸广泛存在于禾谷类和豆类等作物种子中,近年来利用诱变技术选育低植酸作物新品种已成研究热点。本研究在不同 NaCl 浓度处理下,对前期通过60 Co -γ辐射获得的9份低植酸小麦突变体材料进行萌发试验。结果表明,0.5%NaCl 能促进种子萌发,而1.0%、1.5%NaCl 明显抑制种子萌发,在其胁迫下,种子幼苗和主根生长受到抑制。初步筛选到耐盐性较好的低植酸小麦突变体材料4份,分别为 lp14、lp49、lp55、lp50。

  20. Effect of phytic acid ketone on expression of HPV16/18 E6/E7 mRNA and protein in human cervical cancer cell line%植酸酮对宫颈癌细胞株中 HPV16/18 E6/E7 mRNA 及蛋白表达的影响

    Institute of Scientific and Technical Information of China (English)

    王侃; 高晓丽; 田文艳; 岳天孚

    2016-01-01

    Objective To observe the effects of phytic acid ketone on expression of HPV 16/18 E6/E7 mRNA and protein in human cervical cancer cells.Methods The Caski cells ( containing HPV16 ) and Hela cells ( containing HPV18) were cultivated in vitro and then divided into the experimental groups 1, 2, 3, 4 and the control group ( each group contained Caski cell line and Hela cell line).The experimental groups 1, 2, 3, 4 were respectively treated with dif-ferent concentrations of phytic acid ketone (58.6 mg/L, 117 mg/L, 586 mg/L, 5 860 mg/L) for 72 h.The levels of HPV16/18 E6/E7 mRNA were determined with real-time PCR.Western blotting was used to measure the expression levels of E6 and E7 proteins.Results E6/E7 mRNA and protein expression in the same type of cells of the experimental groups 1, 2, 3 and 4 was lower than that of the control group, and the E6/E7 mRNA and protein expression showed a decreasing tendency in the experimental groups 1, 2, 3 and 4 (all P<0.05).In each experimental group, E6/E7 mRNA expression in Caski cells was lower than that in Hela cells (all P<0.05).In the same type of cells of the experiential groups, the E6 mRNA expression was lower than that of E7 mRNA (all P<0.05).Conclusions Phytic acid ketone could down-regulate the expression of E6/E7 mRNA and protein in human cervical cancer lines, which was in a dose-dependent manner.The inhibitory effect of phytic acid ketone on HPV16 E6/E7 mRNA was stronger than that on HPV18 E6/E7 mRNA and the suppression of E6 probably played a leading role.%目的:观察植酸酮对宫颈癌细胞株中HPV16/18 E6/E7 mRNA及蛋白表达的影响。方法体外培养Caski细胞株(含HPV16)与Hela细胞株(含HPV18),将所有细胞分成实验1、2、3、4组和对照组,每组均包含Caski细胞和Hela细胞。实验1、2、3、4组分别加入含58.6、117、586、5860 mg/L植酸酮的培养液,对照组加入不含植酸酮的培养液。各组培养72 h后,采用real-time PCR

  1. STUDY ON ADSORPTION OF HUMIC AND PHYIC ACIDS USING RESINS

    Institute of Scientific and Technical Information of China (English)

    WANG Jinnan; LI Aimin; ZHOU Youdong; YANG Zhou; LI Xu

    2008-01-01

    @@ 1. INTRODUCTION Humic acid (HA) and Phytic acid (PA) are present in environment as a result of the decomposition of plant biomass, they are the major components of natural dissolved organic matter (DOM) in natural systems[1].

  2. Phytase activity, phytic acid, zinc, phosphorus and protein contents ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-09-15

    Sep 15, 2009 ... functions and the central nervous system, susceptibility to infection, impaired ... balance data, approximately 20.5% of the world's popu- lation is estimated to be at ... Asia (26.7%) and Latin America and the Caribbean. (24.8%) .... water ratio), 1.8% organic matter (Walkey and Black method,. Jackson, 1973) ...

  3. Lipid stability of ham and fresh sausages of pigs treated with diets containing high levels of phytic acidEstabilidade lipídica do pernil e da linguiça frescal de suínos tratados com dietas com alta concentração de ácido fítico

    Directory of Open Access Journals (Sweden)

    Piero Agostini da Silva

    2011-12-01

    Full Text Available The experiment evaluated the effects of different periods of inclusion of defatted corn germ meal (DCGM, as a principal ingredient and source of phytic acid in the rations of finishing swine, on the chemical and sensorial characteristics and on the preservation of lipid oxidation of ham and the fresh sausage. Twenty four borrows with 75.408 ± 4.407 kg of initial weight and 123 days of age, with the same genetic basis were used. The experimental treatments were represented by the inclusion of 50% of DCGM in the finishing swine rations during 0, 7, 14 and 21 days before of slaughter, where were defined four treatments. The pigs were fed ad libitum during 28 days. After the slaughter, it was collected the ham of the half carcass and samples of the ham’s muscles were used to elaborate the fresh sausages. The approximate chemical composition, the color and the lipid stability of ham and fresh sausage were evaluated. The experimental design was a randomized blocks (based on initial pigs weight with 6 replications per treatment. There were no regression effects over the evaluated parameters, where the DCGM did not affect the approximate chemical composition and the color of the samples. There were no differences between the treatments for ham and fresh sausage for the lipid stability according the different periods of inclusion of DCGM. The high inclusion of DCGM in the finishing pig rations until 21 days before the slaughter didn’t improve the quality characteristics and the oxidation of ham and fresh sausage. experimento avaliou o efeito de diferentes períodos de inclusão do farelo de gérmen de milho desengordurado (FGMD, como principal ingrediente e fonte de ácido fítico em rações de suínos em fase de terminação, sobre as características químicas, sensoriais e de preservação da oxidação no pernil e na linguiça frescal. Foram utilizados 24 suínos machos de mesma genética comercial com peso médio inicial de 75,408 ± 4,407 kg

  4. Cyanic acid, Phytic acid, total tannin and aflatoxin contents of a Brazilian (Natal multimistura preparation Conteúdos de ácido cianídrico, ácido fítico, tanino total e aflatoxina em uma preparação brasileira (Natal de multimistura

    Directory of Open Access Journals (Sweden)

    Francisca Sônia CÂMARA

    2001-04-01

    Full Text Available The utilization of a "multimistura" as food supplement, in nutritional improvement programs for the low-income population in Brazil, represents a low-cost and easy-to-use food preparation alternative, presentig reasonable nutritive value and satisfying local taste preferences. This is based on the fact that the "multimistura" is made up of non-conventional ingredients/foods and of agroindustrial by-products, rich in different nutrients. This paper was designed to determine some toxic and/or antinutritional factors of a "Multimistura" utilized as food supplement in institutional programs to prevent malnutrition by the Department of Welfare and Social Affairs of the City of Natal, RN. The product studied had the following formulation: 30% of wheat bran; 30% of wheat flour; 30% of corn meal; 3% of powder from cassava leaves; 4% of pumpkin seeds powder; and 3% of eggshell powder. The analysis of the main toxic and/or antinutritional factors showed very low concentrations of phytates and tannins and no detectable levels of aflatoxin and cyanic acid.O uso da "multimistura" como suplemento alimentar, em programas de intervenção nutricional para populações brasileiras carentes, vem se apresentando como uma alternativa alimentar de valor nutritivo razoável, baixo custo, preparo rápido e paladar regionalizado. Isto resulta do fato desta ser constituída principalmente de alimentos não convencionais e/ou subprodutos agro-industriais ricos em diferentes nutrientes. Este trabalho teve como objetivo dosar alguns fatores tóxicos e/ou antinutricionais de uma "multimistura" utilizada como suplemento alimentar em programas institucionais no combate à desnutrição da Secretaria Municipal de Promoção Social da cidade de Natal. O produto analisado tinha a seguinte formulação: 30% de farelo de trigo; 30% de farinha de trigo; 30% de fubá de milho; 3% de pó de folha de mandioca; 4% de pó de semente de jerimum; 3% de pó de casca de ovo. As an

  5. A novel phytase with sequence similarity to purple acid phosphatases is expressed in cotyledons of germinating soybean seedlings.

    Science.gov (United States)

    Hegeman, C E; Grabau, E A

    2001-08-01

    Phytic acid (myo-inositol hexakisphosphate) is the major storage form of phosphorus in plant seeds. During germination, stored reserves are used as a source of nutrients by the plant seedling. Phytic acid is degraded by the activity of phytases to yield inositol and free phosphate. Due to the lack of phytases in the non-ruminant digestive tract, monogastric animals cannot utilize dietary phytic acid and it is excreted into manure. High phytic acid content in manure results in elevated phosphorus levels in soil and water and accompanying environmental concerns. The use of phytases to degrade seed phytic acid has potential for reducing the negative environmental impact of livestock production. A phytase was purified to electrophoretic homogeneity from cotyledons of germinated soybeans (Glycine max L. Merr.). Peptide sequence data generated from the purified enzyme facilitated the cloning of the phytase sequence (GmPhy) employing a polymerase chain reaction strategy. The introduction of GmPhy into soybean tissue culture resulted in increased phytase activity in transformed cells, which confirmed the identity of the phytase gene. It is surprising that the soybean phytase was unrelated to previously characterized microbial or maize (Zea mays) phytases, which were classified as histidine acid phosphatases. The soybean phytase sequence exhibited a high degree of similarity to purple acid phosphatases, a class of metallophosphoesterases.

  6. Genetic Architecture of Grain Chalk in Rice and Interactions with a Low Phytic Acid Locus

    Science.gov (United States)

    Grain quality characteristics have a major impact on the value of the harvested rice crop. In addition to grain dimensions which determine rice grain market classes, translucent milled kernels are also important for assuring the highest grain quality and crop value. Over the last several years, ther...

  7. Genetic architecture of grain chalk in rice and interactions with a low phytic acid locus

    Science.gov (United States)

    Grain quality characteristics have a major impact on the value of the harvested rice crop. In addition to grain dimensions which determine market classes, translucency is also required for the highest grain quality. Over the last several years, the USA rice industry has been concerned about the incr...

  8. Effect of processing on proximate composition, anti-nutrient status and amino acid content in three accessions of African locust bean (Parkia biglobosa (jacq.) benth.

    Science.gov (United States)

    Urua, Ikootobong Sunday; Uyoh, Edak Aniedi; Ntui, Valentine Otang; Okpako, Elza Cletus

    2013-02-01

    Proximate composition, amino acid levels and anti-nutrient factors (polyphenols, phytic acid and oxalate) in the seeds of Parkia biglobosa were determined at three stages: raw, boiled and fermented. The highest anti-nutrient factor present in the raw state was oxalate, while phytic acid was the least. The amino acid of the raw seeds matched favourably to the World Health Organization reference standard. After processing, boiling increased fat, crude fibre and protein, while it reduced moisture, ash and the anti-nutrient content in 64% of the cases examined. Fermentation reduced ash, crude fibre and carbohydrate in all the accessions. It increased the moisture, fat and protein, while reducing the anti-nutrient factors in 78% of the cases. The high levels of protein, fat and amino acids coupled with the low levels of the anti-nutrients in the boiled and fermented seeds make Parkia a good source of nutrients for humans and livestock.

  9. Bioavailability of iron, zinc, folic acid, and vitamin A from fortified maize.

    Science.gov (United States)

    Moretti, Diego; Biebinger, Ralf; Bruins, Maaike J; Hoeft, Birgit; Kraemer, Klaus

    2014-04-01

    Several strategies appear suitable to improve iron and zinc bioavailability from fortified maize, and fortification per se will increase the intake of bioavailable iron and zinc. Corn masa flour or whole maize should be fortified with sodium iron ethylenediaminetetraacetate (NaFeEDTA), ferrous fumarate, or ferrous sulfate, and degermed corn flour should be fortified with ferrous sulfate or ferrous fumarate. The choice of zinc fortificant appears to have a limited impact on zinc bioavailability. Phytic acid is a major inhibitor of both iron and zinc absorption. Degermination at the mill will reduce phytic acid content, and degermed maize appears to be a suitable vehicle for iron and zinc fortification. Enzymatic phytate degradation may be a suitable home-based technique to enhance the bioavailability of iron and zinc from fortified maize. Bioavailability experiments with low phytic acid-containing maize varieties have suggested an improved zinc bioavailability compared to wild-type counterparts. The bioavailability of folic acid from maize porridge was reported to be slightly higher than from baked wheat bread. The bioavailability of vitamin A provided as encapsulated retinyl esters is generally high and is typically not strongly influenced by the food matrix, but has not been fully investigated in maize.

  10. Effects of a 6-phytase on the apparent ileal digestibility of minerals and amino acids in ileorectal anastomosed pigs fed on a corn-soybean meal-barley diet.

    Science.gov (United States)

    Guggenbuhl, P; Waché, Y; Simoes Nunes, C; Fru, F

    2012-12-01

    Phosphorus of plant-based feedstuffs for monogastric animals is mainly in the form of phytic P, which has a very low bioavailability. The nondigested phytic P may contribute to P pollution. Furthermore, phytic acid may reduce digestibility of other minerals and protein. This study evaluated effects of the microbial 6-phytase RONOZYME HiPhos on apparent ileal digestibility of P, phytic acid, Ca, CP, energy, and AA in six 60-d-old ileorectal anastomosed pigs. In a duplicated 3 × 3 Latin square design, pigs had free access to alternatively a corn (Zea mays)-soybean (Glycine max) meal-barley (Hordeum vulgare)-based diet or this diet supplemented with RONOZYME HiPhos at either 500 units/kg (RH500) or 1000 units/kg (RH1000). Pigs fed diets supplemented with RH500 or RH1000 increased (P phytase increased apparent ileal digestibility of these indispensable minerals and phytate. The phytase increased digestibility of CP and indispensable AA indicating a better availability of plant-based proteins.

  11. In Vivo Antioxidantion Effect of Phytic Acid%植酸的体内抗氧化活性研究

    Institute of Scientific and Technical Information of China (English)

    陈园; 仇农学; 熊犍

    2011-01-01

    将Specific pathogen free(SPF)级昆明小鼠随机分为药物组和正常对照组,分别灌胃植酸和生理盐水,30 d后经眼球职血和颈椎脱臼法处死,制取血清和肝组织匀浆,并测定其中总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)的活性和丙二醛(MDA)的含量.结果表明:中剂量试验组小鼠的血清、肝组织中SOD、GSH-Px、CAT等抗氧化酶活性明显高于正常对照组,其MDA含量也明显降低;T-AOC的高剂量组呈现出雌雄细的差异,说明植酸在一定的剂量范围内可以增强机体的抗氧化机能,延缓衰老.

  12. Concerted action of endogenous and heterologous phytase on phytic acid degradation in seed of transgenic wheat (Triticum aestivum L.).

    Science.gov (United States)

    Brinch-Pedersen, Henrik; Hatzack, Frank; Sørensen, Lisbeth D; Holm, Preben B

    2003-12-01

    Expression of heterologous phytases in crops offers a great potential for improving phosphate and mineral bioavailability in food and feed. In this context it is of relevance to describe the concerted action of endogenous and hetrologous phytases on the transgenic seed inositol phosphate profile. Here we report metal-dye detection HPLC analysis of inositol phosphate degradation in flour from transgenic wheat materials possessing wheat endogenous 6-phytase [EC 3.1.3.26] and Aspergillus 3-phytase [EC 3.1.3.8] activities under the control of the maize ubiquitin-1 promoter and the wheat high molecular weight glutenin subunit 1DX5 promoter respectively. During 50 min incubation there is an accumulation of InsP5 to InsP2 breakdown products in non-transgenic material. Aspergillus niger phytase specific breakdown products are transiently detected in transgenic material but after 50 min incubation virtually all InsP5, InsP4 and InsP3 isomers are hydrolysed.

  13. Validation study of a rapid colorimetric method for the determination of phytic acid and inorganic phosphorus from seeds

    OpenAIRE

    Dragičević Vesna D.; Sredojević Slobodanka D.; Perić Vesna A.; Nišavić Anika R.; Srebrić Mirjana B.

    2011-01-01

    Phytate, as an important mineral storage compound in seeds, is vital for seed/grain development; it is often considered to be an antinutritional substance. The objective of this study was to develop a rapid and inexpensive colorimetric method of measuring phytate and inorganic P (Pi) concentrations from maize, soybean and sunflower seed/grain extracts, by combining adequate precision and simplicity, ideal for breeders interested in improving simultaneously Pi and phytate levels. The inv...

  14. Fermentation of phytic acid from lamtoro gung seeds (Leucaena leucocephala to produce inositol and tannin as pharmaceutical commodity

    Directory of Open Access Journals (Sweden)

    I. A.R. Bakti

    2003-12-01

    Full Text Available The research was done to define the effect of tempe yeast concentration on inositol and tannin contents of fermented lamtoro gung seeds (Leucaena leucocephala. This is a research with completely randomized design using four doses of tempe yeast, i.e. 0 %, 5 %, 10 %, and 15 %. Each treatment was replicated 4 times. The resulted data were analyzed by analysis of variance and the significant differences were tested by least significantly difference test. Tannin was analyzed by Lowenthal-Procter method. The result of the research showed that the treatment had significant effects (P<0.05 on the parameter measured. The highest inositol with lowest tannin contents were found by using 15 % tempe yeast and duration of fermentation 96 hours, that is 0.2631 %. (Med J Indones 2003; 12: 236-42Keywords: tempe yeast

  15. Uric acid urolithiasis and crystallization inhibitors.

    Science.gov (United States)

    Grases, F; Ramis, M; Villacampa, A I; Costa-Bauzá, A

    1999-01-01

    An in vitro study of the inhibitory effects that some substances occasionally present in urine can provoke on the crystallization of uric acid has been performed. The most remarkable crystallization inhibitory effects were produced by mucine at concentrations of >0.5 mg/l. Pentosan polysulfate and chondroitin sulfate also clearly increased the uric acid crystallization times at concentrations of >100 mg/l. Saponins, such as escin and glycyrrhizic acid, also produced a notable delay in uric acid crystallization times at concentrations of >10 mg/l. Similar effects were observed in the presence of a surfactant substance, lauryl sulfate. N-Acetyl-L-cysteine caused crystallization perturbations only when it was present at concentrations of >50 mg/l. Citric acid and phytic acid caused no effects on uric acid crystallization even at the highest concentrations assayed (1,000 and 5 mg/l, respectively). From the results obtained it can be deduced that mainly glycoproteins, glycosaminoglycans and surfactant substances can exert protective effects against uric acid crystallization.

  16. 3D Polyaniline Architecture by Concurrent Inorganic and Organic Acid Doping for Superior and Robust High Rate Supercapacitor Performance.

    Science.gov (United States)

    Gawli, Yogesh; Banerjee, Abhik; Dhakras, Dipti; Deo, Meenal; Bulani, Dinesh; Wadgaonkar, Prakash; Shelke, Manjusha; Ogale, Satishchandra

    2016-02-12

    A good high rate supercapacitor performance requires a fine control of morphological (surface area and pore size distribution) and electrical properties of the electrode materials. Polyaniline (PANI) is an interesting material in supercapacitor context because it stores energy Faradaically. However in conventional inorganic (e.g. HCl) acid doping, the conductivity is high but the morphological features are undesirable. On the other hand, in weak organic acid (e.g. phytic acid) doping, interesting and desirable 3D connected morphological features are attained but the conductivity is poorer. Here the synergy of the positive quality factors of these two acid doping approaches is realized by concurrent and optimized strong-inorganic (HCl) and weak-organic (phytic) acid doping, resulting in a molecular composite material that renders impressive and robust supercapacitor performance. Thus, a nearly constant high specific capacitance of 350 F g(-1) is realized for the optimised case of binary doping over the entire range of 1 A g(-1) to 40 A g(-1) with stability of 500 cycles at 40 A g(-1). Frequency dependant conductivity measurements show that the optimized co-doped case is more metallic than separately doped materials. This transport property emanates from the unique 3D single molecular character of such system.

  17. 3D Polyaniline Architecture by Concurrent Inorganic and Organic Acid Doping for Superior and Robust High Rate Supercapacitor Performance

    Science.gov (United States)

    Gawli, Yogesh; Banerjee, Abhik; Dhakras, Dipti; Deo, Meenal; Bulani, Dinesh; Wadgaonkar, Prakash; Shelke, Manjusha; Ogale, Satishchandra

    2016-02-01

    A good high rate supercapacitor performance requires a fine control of morphological (surface area and pore size distribution) and electrical properties of the electrode materials. Polyaniline (PANI) is an interesting material in supercapacitor context because it stores energy Faradaically. However in conventional inorganic (e.g. HCl) acid doping, the conductivity is high but the morphological features are undesirable. On the other hand, in weak organic acid (e.g. phytic acid) doping, interesting and desirable 3D connected morphological features are attained but the conductivity is poorer. Here the synergy of the positive quality factors of these two acid doping approaches is realized by concurrent and optimized strong-inorganic (HCl) and weak-organic (phytic) acid doping, resulting in a molecular composite material that renders impressive and robust supercapacitor performance. Thus, a nearly constant high specific capacitance of 350 F g‑1 is realized for the optimised case of binary doping over the entire range of 1 A g‑1 to 40 A g‑1 with stability of 500 cycles at 40 A g‑1. Frequency dependant conductivity measurements show that the optimized co-doped case is more metallic than separately doped materials. This transport property emanates from the unique 3D single molecular character of such system.

  18. Studies on Synthesis of Electrochemically Exfoliated Functionalized Graphene and Polylactic Acid/Ferric Phytate Functionalized Graphene Nanocomposites as New Fire Hazard Suppression Materials.

    Science.gov (United States)

    Feng, Xiaming; Wang, Xin; Cai, Wei; Qiu, Shuilai; Hu, Yuan; Liew, Kim Meow

    2016-09-28

    Practical application of functionalized graphene in polymeric nanocomposites is hampered by the lack of cost-effective and eco-friendly methods for its production. Here, we reported a facile and green electrochemical approach for preparing ferric phytate functionalized graphene (f-GNS) by simultaneously utilizing biobased phytic acid as electrolyte and modifier for the first time. Due to the presence of phytic acid, electrochemical exfoliation leads to low oxidized graphene sheets (a C/O ratio of 14.8) that are tens of micrometers large. Successful functionalization of graphene was confirmed by the appearance of phosphorus and iron peaks in the X-ray photoelectron spectrum. Further, high-performance polylactic acid/f-GNS nanocomposites are readily fabricated by a convenient masterbatch strategy. Notably, inclusion of well-dispersed f-GNS resulted in dramatic suppression on fire hazards of polylactic acid in terms of reduced peak heat-release rate (decreased by 40%), low CO yield, and formation of a high graphitized protective char layer. Moreover, obviously improvements in crystallization rate and thermal conductivities of polylactic acid nanocomposites were observed, highlighting its promising potential in practical application. This novel strategy toward the simultaneous exfoliation and functionalization for graphene demonstrates a simple yet very effective approach for fabricating graphene-based flame retardants.

  19. 植酸对黄曲霉菌(Aspergillus flavus)糖化力的影响%Effects of Phytic Acid on Saccharification of Aspergillus flavus

    Institute of Scientific and Technical Information of China (English)

    汪志君; 夏艳秋; 方维明; 朱强

    2003-01-01

    该文通过添加不同浓度的植酸于黄曲霉菌(Aspergillus flavus)培养基中,用不同添加方式,于不同生长时刻测定黄曲霉菌糖化力,研究植酸对黄曲霉菌糖化力的影响.结果表明加入植酸后黄曲霉菌糖化力均有很大提高,可为工业生产带来可观的经济效益.植酸最佳作用浓度范围是0.20%~0.25%,发酵24h糖化力最大可提高44.32%.

  20. The effects of phytase supplementation on performance and phosphorus excretion from broiler chickens fed low phosphorus-containing diets based on normal or low-phytic acid barley

    Science.gov (United States)

    : Long term application of poultry manure can lead to soil phosphorus (P) accumulation and the potential P transport into waterbodies which can contribute to eutrophication of freshwater systems. One reason for this accumulation of soil P is due to the inefficiency of poultry to utilize the P in f...

  1. STUDY ON ENZYMATIC REMOVAL OF PHYTIC ACID IN SOYBEAN%大豆中植酸的酶法去除研究

    Institute of Scientific and Technical Information of China (English)

    顾振宇; 励建荣; 顾青; 王莹; 王胜朝

    2000-01-01

    利用小麦浸泡萌发时其内部的植酸酶去除大豆中的植酸.将大豆与发芽小麦按4:6的比例(干基)混合,打浆后,在56~58℃,pH5.0~5.2条件下处理3h,植酸的去除率可达97%以上.

  2. 酶法去除大豆植酸的正交分析%Orthogonal Test of Removaing Phytic Acid in Soybean Using Endogenous Enzyme of Barely

    Institute of Scientific and Technical Information of China (English)

    付家庆; 周海军; 庞玉艳

    2008-01-01

    利用大麦浸泡萌发时其内部的植酸酶去除大豆中的植酸.在单因素基础上进行正交试验,将侵泡1d大豆与发芽3d大麦4∶6混合,打浆后,在60℃,pH值6.0条件下酶解3h麦豆混合物中植酸去除率可达96%.

  3. STUDY ON REMOVING PHYTIC ACID IN SOYBEAN USING ENDOGENOUS ENZYME OF BARLEY%大麦内源酶去除大豆植酸的研究

    Institute of Scientific and Technical Information of China (English)

    南昌希; 周丽萍

    2005-01-01

    利用发芽大麦的植酸酶去除大豆中的植酸.试验表明:将发芽3d的大麦与浸泡1d的大豆以6:4的比例混合粉碎后,在55~60℃,pH=5.5条件下酶解3h,麦豆混合物中植酸去除率可达93%以上.

  4. 发芽大豆中植酸的测定方法优化%Optimization of Determination Method of Phytic Acid of Germinated Soybean

    Institute of Scientific and Technical Information of China (English)

    仲磊; 王新坤; 杨润强; 顾振新

    2014-01-01

    探讨了以DEAE Sepharose FF阴离子交换树脂为纯化介质的大豆植酸快速测定方法,主要对测定过程中植酸的纯化、消化方法进行了优化.在提取方法的基础上(料液比1∶50(质量体积比)),6 mL的树脂体积能够充分交换上样液中的植酸;用15 mL蒸馏水、15 mL 0.05 mol/L NaCl淋洗,能够较好达到除游离磷效果;植酸溶液经过消化炉300℃浓缩后,加入3 mL浓硫酸以400℃消化0.5h,回收率达到90%以上.此方法的加标回收率达108.4%,3个大豆样品中植酸测定结果的相对标准偏差分别为4.91%、2.82%和1.24%.

  5. Studies on removal of phytic acid from soybean by germination method%发芽方法去除大豆中植酸的研究

    Institute of Scientific and Technical Information of China (English)

    苗颖; 马莺

    2010-01-01

    研究利用发芽的方法使大豆中的植酸含量下降,以植酸为评价指标采用正交试验确定大豆最佳发芽条件,同时测定大豆在温度30℃、发芽3 d过程中游离氨基酸、游离脂肪酸和VC含量的变化.结果表明:温度是影响植酸的主要因素,时间次之;大豆发芽的最佳条件为温度30℃、时间3 d;此时植酸含量为2.68 g/L,降低率达62.8%.

  6. 酶法水解大豆中植酸的研究%A Study on Phatase Enzymolysis of Phytic Acid in Soybean

    Institute of Scientific and Technical Information of China (English)

    吴金鸿; 陈娟; 林向阳; 倪莉; 饶平凡

    2004-01-01

    探讨了植酸酶在不同酶解条件(pH值、温度、时间、加酶量)下对大豆抗营养因子--植酸盐的水解作用.研究结果表明,适宜的酶解条件为:温度37℃,酶加量3 750U/kg豆粉,酶解时间45min,pH值6.1.在此条件下,酶解后每克大豆粉游离无机磷的含量增加208μmol(约占总磷的66.4%);可溶性金属离子Zn2+、Ca2+、Mg2+和Fe2+的释放量分别达到73.0%、69.6%、62.9%和23.7%;植酸的降解率达到56.6%.结论:酶法水解大豆中植酸可以显著提高大豆的营养效价.

  7. 大豆低植酸育种研究进展%Review for Study of Low Phytic Acid Breeding in Soybean

    Institute of Scientific and Technical Information of China (English)

    袁凤杰; 舒庆尧; 朱丹华

    2006-01-01

    大豆种子中的磷主要以植酸的形式存在,它对人和单胃动物来说是一种抗营养因子;降低植酸的含量不仅可以提高大豆微量元素、蛋白质和磷素的生物有效性,而且可以减少磷素的污染和浪费.就不同大豆品种中植酸的含量、低植酸种质的创新,以及这些种质的遗传机制、农艺性状、营养品质和分子特性的研究给予了较为全面的综述.

  8. Metabolite changes during natural and lactic acid bacteria fermentations in pastes of soybeans and soybean-maize blends.

    Science.gov (United States)

    Ng'ong'ola-Manani, Tinna Austen; Ostlie, Hilde Marit; Mwangwela, Agnes Mbachi; Wicklund, Trude

    2014-11-01

    The effect of natural and lactic acid bacteria (LAB) fermentation processes on metabolite changes in pastes of soybeans and soybean-maize blends was studied. Pastes composed of 100% soybeans, 90% soybeans and 10% maize, and 75% soybeans and 25% maize were naturally fermented (NFP), and were fermented by lactic acid bacteria (LFP). LAB fermentation processes were facilitated through back-slopping using a traditional fermented gruel, thobwa as an inoculum. Naturally fermented pastes were designated 100S, 90S, and 75S, while LFP were designated 100SBS, 90SBS, and 75SBS. All samples, except 75SBS, showed highest increase in soluble protein content at 48 h and this was highest in 100S (49%) followed by 90SBS (15%), while increases in 100SBS, 90S, and 75S were about 12%. Significant (P phytic acid (85% in NFP and 49% in LFP by 72 h).

  9. Hydrolysis of phytic acid by intrinsic plant and supplemented microbial phytase (Aspergillus niger) in the stomach and small intestine of minipigs fitted with re-entrant cannulas. 3. Hydrolysis of phytic acid (IP6) and occurrence of hydrolysis products (IP5, IP4, IP3 and IP2).

    Science.gov (United States)

    Rapp, C; Lantzsch, H J; Drochner, W

    2001-12-01

    Hydrolysis of phytate in the stomach and the small intestine as influenced by intrinsic plant (wheat) and supplemented microbial phytase (Aspergillus niger) were investigated with six minipigs (40-50 kg initial body weight) fitted with re-entrant cannulas in the duodenum, 30 cm posterior to the pylorus (animals 1, 4, 5 and 6) and ileocecal re-entrant cannulas, 5 cm prior the ileocecal junction (animals 1, 2 and 3), respectively. Dietary treatments were as follows: (1) diet 1, a corn-based diet [43 U phytase/kg dry matter (DM)]; (2) diet 2, diet 1 supplemented with microbial phytase (818 U/kg DM); and (3) diet 3, a wheat-based diet (1192 U/kg DM). At 07 30 h and 19 30 h, each animal was fed 350 g diet mixed with 1050 ml de-ionized water. Digesta were collected continuously and completely during a 12-h period after feeding. Mean hydrolysis rates of IP6 in the stomach as measured at the proximal duodenum of animals 1, 4, 5 and 6 were 9.0, 77.2 and 66.2% for diet 1, 2 and 3, respectively. Microbial phytase was much more effective in phytate hydrolysis than wheat phytase. Mean IP6 hydrolysis rates of the respective diets in the stomach and small intestine as measured at the distal ileum of animals 1, 2 and 3 were 19.0, 62.6 and 64.6% and were lower than treatment means of the stomach only. Differences existed between experimental animals with respect to their ability to hydrolyse IP6 in the stomach independent of the presence and source of dietary phytase. Considerable amounts of hydrolysis products occurred in both the duodenal and ileal digesta when diets 2 and 3 were fed; however, only traces were determined after ingestion of diet 1. Independent of dietary treatment, four IP5 isomers were detected, but in different amounts.

  10. 植酸酶对玉米、豆粕日粮中生长猪代谢的影响%Effects of low-phytic acid corn,low-phytic acid soybean meal,and phytase on nutrient digestibility and excretion in growing pigs

    Institute of Scientific and Technical Information of China (English)

    李春红

    2010-01-01

    本文通过植酸酶对生长猪的代谢的不同来评估饲喂低植酸玉米、低植酸豆粕、常规玉米、常规豆粕对其的影响.将48头去势的生长猪随机分成8个组,每组6头猪,采用2×2×2的试验设计,猪只日粮均符合日粮标准.结果表明,低植酸玉米组比常规玉米组(2.85:3.24±0.119g/d;P=0.024),低植酸豆粕组比常规豆粕组(2.79:3.30±0.119g/d;P=0.007),植酸酶组比不饲喂植酸酶组(2.80:3.29±0.119g/d;P=0.009),低植酸玉米、豆粕、添加植酸酶组比常规玉米、豆粕和无植酸酶组(2.16:3.70±0.237g/d;P<0.001)的粪中的磷都要低.低植酸玉米日粮比常规玉米日粮(48.4:39.9±2.27%;P=0.012).植酸酶日粮比无植酸酶日粮(48.4:39.9±2.27%;P=0.019),低植酸玉米、豆粕、添加植酸酶组比常规玉米、豆粕和无植酸酶组日粮(47.2:41.1±2.27%;P=0.075)的磷的消化率都有所增加,这些说明低植酸玉米、低植酸豆粕或者直接添加植酸酶酶制剂均可以提高磷的利用率并且可以通过粪中磷的降低而改善对环境的污染问题.

  11. POTENTIAL OF CEREALS AND PSEUDOCEREALS FOR LACTIC ACID FERMENTATIONS

    Directory of Open Access Journals (Sweden)

    Ľubomír Valík

    2011-04-01

    Full Text Available Cereals and pseudocereals play a significant role in human nutrition. They are source of specific carbohydrates, proteins, lipids, fibre and wide spectrum of vitamins and minerals. Moreover, pseudocereals have a higher content of essential amino acids, e.g. lysine and tryptophan. Cereals and pseudocereals may also contain some antinutrition factors, such as phytic acid, polyphenols, trypsin inhibitors and inhibitors of α-amylase. These are responsible for reducing of protein and carbohydrate digestibility and decreasing accessibility of minerals due to complex formation. This review assesses the applications of cereals and pseudocereals in fermentation technology including the effects of lactic acid bacteria on nutrition, sensory quality and shelf-life. This work is focusing also on fermentation process of cereal matrice leading in degradation of antinutritional factors increase of nutritional value and availability of minerals, proteins and carbohydrates. Lactic acid bacteria produce many aromatic compounds that are beneficial to organoleptic atributes of the products. However, a few questions have been not answered in experiments, yet. For eample, is there any space for evaluation of their suitability to act as carriers of probiotics? Could such the attempts lead in development some special formulae suitable for consumers with food allergies or deficiencies?doi:10.5219/127

  12. Determining phytic acid in soybean samples with ion chromatography with an eluent in low acidity%低酸度淋洗离子色谱法测定大豆中的植酸

    Institute of Scientific and Technical Information of China (English)

    黄慧

    2003-01-01

    本文以含Ca2+、Mg2+的溶液为淋洗液,以Fe(Ⅱ)-磺基水杨酸为柱后衍生体系,在国产分离柱上分离了磷酸与植酸.利用Ca2+、Mg2+与植酸的络合作用缩短了植酸的保留时间,使植酸可在低酸度(pH=4)下被洗脱,实现了植酸的快速离子色谱分析.利用此法,测定大豆样品中植酸的含量,得到满意的结果.

  13. A game of keep-away: feeding the fish and not the pond

    Science.gov (United States)

    Phytic acid is a storage form of phosphorus in many plants. Its levels rise in the diet along with the inclusion rate of plant-based materials. Our measurements have found that phytic acid levels can be as high as 2% of catfish diets (40 lbs/ton of feed). However, phytic acid is largely indigestib...

  14. Acidic deposition ("acid rain")

    Science.gov (United States)

    Schreiber, R. Kent; LaRoe, Edward T.; Farris, Gaye S.; Puckett, Catherine E.; Doran, Peter D.; Mac, Michael J.

    1995-01-01

    Acidic deposition, or "acid rain," describes any form of precipitation, including rain, snow, and fog, with a pH of 5.5 or below (Note: pH values below 7 are acidic; vinegar has a pH of 3). It often results when the acidity of normal precipitation is increased by sulfates and nitrates that are emitted into the atmosphere from burning fossil fuels. This form of airborne contamination is considered harmful, both directly and indirectly, to a host of plant and animal species.Although acid rain can fall virtually anywhere, ecological damages in environmentally sensitive areas downwind of industrial and urban emissions are a major concern. This includes areas that have a reduced capacity to neutralize acid inputs because of low alkalinity soils and areas that contain species with a low tolerance to acid conditions. To determine the distribution of acidic deposition and evaluate its biological effects, research and monitoring are being conducted by the federal government with support from states, universities, and private industry.            The national extent of the acid rain problem has been estimated by sampling water from 3,000 lakes and 500 streams (Irving 1991), representing more than 28,000 lakes and 56,000 stream reaches with a total of 200,000 km (125,000 mi). Some particularly sensitive areas, such as the Adirondack Mountain region, have been more intensively sampled and the biota examined in detail for effects from acidity.         To identify trends in aquatic ecosystems, present and historical survey data on water chemistry and associated biota are compared. In lakes, the chemical and biological history and pH trends may be inferred or reconstructed in some cases by examining assemblages of fossil diatoms and aquatic invertebrates in the sediment layers. In terrestrial ecosystems, vegetation damage is surveyed and effects of acidic deposition to plants and animals are determined from laboratory and field exposure experiments. Natural

  15. Physical, morphological and chemical characteristics, oil recovery and fatty acid composition of Balanites aegyptiaca Del. kernels.

    Science.gov (United States)

    Mohamed, A M; Wolf, W; Spiess, W E L

    2002-01-01

    Balanites aegyptiaca Del. kernels were chemically, physically and morphologically characterized. Crude oil (49.0%) and crude protein (32.4%) were the two major constituents of the kernels. Phytic acid content was relatively high compared to other legumes. In contrast, antitryptic activities of the kernel flours were very low. Sapogenin contents of the full fat, defatted and testa flours were 1.5, 2.7 and 3.0%, respectively. The hardness of the kernel was found to be about 10.4 x 10(5) N/m2, which was somewhat high. The morphological structure of the kernel using a scanning electron microscope revealed that the protein matrix was embedded in a lake of oil droplets. Oil recovery, as a function of pressing time, pressure, temperature and particle size was investigated. With increasing temperature up to 70 degrees C at 400 bar, for 120 min, an oil recovery of 79.4% was obtained. Using an expeller at 115 degrees C, about 85% of the kernel oil was recovered. The reduction of particle size had a negative effect on oil recovery under the same conditions. The fatty acid composition was not affected by the pressing temperature up to 115 degrees C. The total amount of the unsaturated fatty acids was found to be up to 74.8% (50 degrees C) and 75.1% (115 degrees C) of the total fatty acids content.

  16. Influence of ascorbic acid on iron absorption from an iron-fortified, chocolate-flavored milk drink in Jamaican children.

    Science.gov (United States)

    Davidsson, L; Walczyk, T; Morris, A; Hurrell, R F

    1998-05-01

    The influence of ascorbic acid on iron absorption from an iron-fortified, chocolate-flavored milk drink (6.3 mg total Fe per serving) was evaluated with a stable-isotope technique in 20 6-7-y-old Jamaican children. Each child received two test meals labeled with 5.6 mg 57Fe and 3.0 mg 58Fe as ferrous sulfate on 2 consecutive days. Three different doses of ascorbic acid (0, 25, and 50 mg per 25-g serving) were evaluated in two separate studies by using a crossover design. Iron isotope ratios were measured by negative thermal ionization mass spectrometry. In the first study, iron absorption was significantly greater (P chocolate drink contained relatively high amounts of polyphenolic compounds, phytic acid, and calcium, all well-known inhibitors of iron absorption. The low iron absorption without added ascorbic acid shows that chocolate milk is a poor vehicle for iron fortification unless sufficient amounts of an iron-absorption enhancer are added. Regular consumption of iron-fortified chocolate milk drinks containing added ascorbic acid could have a positive effect on iron nutrition in population groups vulnerable to iron deficiency.

  17. Effetcts of Phytic Acid on Beer Colority Stability under UHP%植酸在超高压条件下对啤酒色度稳定性的影响

    Institute of Scientific and Technical Information of China (English)

    赵玉生; 于然

    2007-01-01

    采用单因素和正交实验,进行了植酸和超高压对啤酒色度的影响研究.结果表明,植酸在高压条件下保持啤酒色度稳定性的最佳工艺条件为:作用压力400 MPa,加压时间5 min,植酸浓度为0.07‰.在此工艺条件下,色度为4.229,比未经处理的对照样品下降了6.44%;植酸、超高压及其协同作用均能保持啤酒色度稳定性.

  18. Nisin和植酸复合抑制腌制蔬菜中G-腐败菌的效果研究%Effect of Nisin and phytic acid in situ on inhibition of Gram negatives in rotten pickle vegetable

    Institute of Scientific and Technical Information of China (English)

    潘丽军; 刘兰花; 曾敏

    2008-01-01

    针对Nisin抑菌谱狭窄的问题,采用复合生物防腐的方法,研究Nisin与植酸复合作用对腌制蔬菜中G-腐败菌的抑制效果.通过对腌制蔬菜中腐败菌的菌种分离纯化与鉴别,得到10种细菌,其中5种G-菌;在菌液浓度为106cfu/mL情况下,进行Nisin与植酸对G-菌抑制效果实验,获得Nisin与植酸复合作用的最佳抑菌浓度为Nisin 1mg/mL,植酸1.5mg/mL.同时,实验证明此复合抑菌浓度对腌制蔬菜中的其它腐败细菌、食品中常见的致病菌大肠杆菌及金黄色葡萄球菌均具有良好的抑制效果.

  19. 植酸钙制备植酸离子交换工艺优化%Study on Preparation of Phytic Acid from Calcium Phytate Through Ion Exchange Process

    Institute of Scientific and Technical Information of China (English)

    郝红英; 邢建华; 周彩荣; 叶文见

    2013-01-01

    在微波辅助提取植酸钙的基础上,利用离子交换法由植酸钙制备植酸,采用单因素试验考察了主要影响因素,得出植酸较佳的离子交换工艺条件为,称取原料植酸钙1 g时,在溶离试验中加3 g树脂;在离子交换过程中,料液流速控制为10.~15 mL/min,交换温度选择为30℃.在此条件下,植酸提取率可达85.53%.

  20. 乳酸链球菌素与植酸大豆蛋白复合膜的功能特性%Functional Properties of Soybean Protein Isolate-Based Complex Films with Nisin and Phytic Acid

    Institute of Scientific and Technical Information of China (English)

    杨万根; 李菲; 李中茂; 朱秋劲; 田维江

    2015-01-01

    为了开发新型大豆蛋白可食膜,将天然减菌剂乳酸链球菌素(Nisin)和植酸加入到大豆蛋白膜中,研究它们对膜的遮光、隔氧、阻湿功能及蛋白质交联度和膜结构的影响.结果表明:添加Nisin会引起大豆分离蛋白膜阻湿性和蛋白质交联度下降,但遮光性和隔氧性增强.植酸对膜的遮光性、阻湿性、隔氧性和蛋白质的交联度的影响与其添加量有关.电镜扫描分析结果表明,大豆分离蛋白膜表面平整光滑,添加了Nisin后,膜中出现复杂纹路,但仍然呈大理石状;继续加入植酸,膜的大理石状结构遭到了破坏.

  1. Dephosphorization of Phytic Acid in Soybean Meal by Using Aspergillus niger Phytase%黑曲霉植酸酶对豆粕中植酸的脱磷作用

    Institute of Scientific and Technical Information of China (English)

    王陶; 李文; 董玉玮; 高明侠; 李同祥

    2016-01-01

    单胃动物不能有效利用植物性饲料中的磷,因为磷主要以植酸磷的形式存在.通常通过在饲料中添加植酸酶,水解植酸脱磷,来提高动物对磷的利用率.利用黑曲霉发酵产生的植酸酶粗酶液对豆粕中的植酸进行脱磷作用研究.通过单因素试验和正交试验确定植酸酶水解豆粕中植酸的最适条件为:酶加量为600 U/g,底物浓度为60 mg/mL,pH为4,温度为55℃,脱去无机磷的量为4.127 mg/g.最优条件下,豆粕中植酸水解51后的水解率为87.34%.

  2. 豆粕和米糠中植酸含量的测定方法研究%Determination of phytic acid in the bean pulp and rice bran

    Institute of Scientific and Technical Information of China (English)

    任传英; 赵永焕; 崔洪斌; 杨志平

    2007-01-01

    采用分光光度法测定豆粕和米糠中的植酸含量.通过正交试验确定了最佳的提取、分离工艺为提取温度45℃、提取时间3 h、D201大孔阴离子交换树脂、流速0.5 mL/min,测得米糠中植酸含量为70.39 mg/g,豆粕中植酸含量为21.42 mg/g.该方法操作简单、准确度较高,适用于农副产品中植酸含量的定量测定.

  3. Ultrasonic assisted in the extraction of phytic acid from soybean residue produced by enzymatic aqueous processing%超声波辅助提取水酶法豆渣中植酸的工艺优化

    Institute of Scientific and Technical Information of China (English)

    江连洲; 毛惠婷; 毕爽; 齐宝坤; 隋晓楠; 王中江; 李佳妮; 李杨

    2016-01-01

    以水酶法提油后的副产物豆渣为原料,探索超声波辅助技术提取植酸的工艺条件,并与振荡浸提法对比.首先选取超声功率、超声温度、超声时间进行单因素实验,通过正交实验选出最佳的超声参数.然后在最优超声参数的基础上,以植酸得率为响应值,利用Box-Behnken中心组合设计原理和响应面分析法,确定提取植酸的最佳条件.结果表明,超声提取植酸的条件为:超声温度50℃,超声功率500 W,超声时间20 min;醋酸浓度4.1%、料液比1∶17 g/mL、提取温度64℃、提取时间33 min.在以上条件下,平均植酸得率为1.23%,与振荡提取法相比,提高41.3%.

  4. Comparative Analysis of Phytic Acid Content of Soybean Varieties in Northeast of China%我国东北地区大豆品种植酸含量的比较分析

    Institute of Scientific and Technical Information of China (English)

    范杰英; 刘香英; 田志刚; 张井勇; 南喜平; 康立宁

    2014-01-01

    对东北地区(黑、吉、辽和内蒙古东部地区)近年来通过审定的183个大豆品种的植酸含量进行了测定分析和比较研究.结果表明,东北地区大豆品种的植酸含量平均为15.58 mg/g,变异区间在9.53~20.16mg/g之间.省份间大豆品种的植酸平均含量也存在差异,以黑龙江省最高,内蒙古最低.大豆品种植酸含量的变化幅度以吉林省最大,黑龙江省和内蒙古均较小.根据测试结果,筛选出1 1个低植酸大豆品种.

  5. Effects of Iron and Phosphorus Nutrition on the Contents of Iron and Phytic Acid in Soybean Seeds%磷铁营养对大豆种子铁和植酸积累的影响

    Institute of Scientific and Technical Information of China (English)

    王贤; 刘晓萌; 杨青春; 陈龙

    2010-01-01

    以铁含量低的周97(Z97)和铁含量高的科丰36(K36)为材料,研究了不同磷铁营养对大豆种子中铁与植酸积累的影响以及植酸与蛋白质的关系.结果表明:不同磷处理极显著地影响种子中植酸与铁的积累,品种间存在一定差异;不同铁处理对种子中铁含量的影响趋势相似, K36受影响的程度明显大于Z97;种子中植酸与铁、植酸与蛋白质均无显著相关性;限制种子中铁积累的主要环节是铁从根向豆荚的运输以及豆荚向种子的转运,并且品种间的遗传差异可以影响铁从豆荚向种子的转运.

  6. 大麦内源酶去除大豆植酸的正交实验研究%Removal of phytic acid in soybean using endogenous enzyme of barely

    Institute of Scientific and Technical Information of China (English)

    张莲姬; 周丽萍; 南昌希

    2006-01-01

    利用发芽大麦的植酸酶去除大豆中的植酸.实验表明,将发芽3d的大麦与浸泡1d的大豆以6:4的比例混合粉碎后,在60℃,pH6.0条件下酶解3h,麦豆混合物中植酸去除率可达96%以上.

  7. The Effect of Separation Method on Determination Result of Phytic Acid Content in Soybean%大豆植酸分离方法对测定结果的影响

    Institute of Scientific and Technical Information of China (English)

    廖明星; 顾振新; 麻浩; 张国正; 梁俭斌

    2003-01-01

    探讨了大豆植酸的浸提、离心和离子交换条件对其测定结果的影响.大豆经磨粉、过筛后,用0.35*#mol/L HCl-0.7*#mol/L Na2SO4溶液能充分提取出大豆的植酸;沸水浴后4*#000*#r/min离心10*#min可除去蛋白质;阴离子交换时,依次用15*#mL蒸馏水、15*#mL 0.05*#mol/L NaCl淋洗,而后用20*#mL 0.7*#mol/L NaCl以1.0*#mL/min的速度洗脱,洗脱液的植酸加标回收率达到98%以上;4个大豆样品中植酸测定结果的相对标准偏差分别为1.23%、4.05%、1.53%和1.83%.

  8. 大豆低植酸突变体Gm-lpa-ZC-2的精细定位%Fine mapping of the Gm-lpa-ZC-2 gene in a low phytic acid soybean mutant

    Institute of Scientific and Technical Information of China (English)

    李佰权; 姜莹; 付旭军; 朱申龙; 朱丹华; 袁风杰

    2011-01-01

    降低大豆植酸含量对于改善大豆营养品质极其重要.通过诱变的方法筛选到大豆低植酸突变体Gm-lpa-ZC-2,经典遗传学分析表明:其突变性状由隐性单基因所控制,并被定位在大豆连锁群B2上.试验在大豆低植酸突变体Gm-lpa-ZC-2初步定位的基础上,通过构建较大的遗传群体,运用生物信息学发展新的连锁标记,结合大豆基因组学和传统定位的方法,进行突变基因的精确定位.结果表明:位于大豆B2连锁群上的Gm-lpa-ZC-2突变基因,与连锁标记Satt168和Satt416的遗传距离远大于初步定位的结果,为18.2 cM;在此基础上,利用大豆基因组序列信息,在Satt168标记的上游序列与目的基因之间的染色体片段,4 900~8 200 kb的区域中,发掘到9对在遗传群体亲本间具有多态性的SSR引物;其中SSR位点psm lpa-224,psm lpa-225和psm lpa-226与目的基因的距离仅为0.8 cM,实现了突变基因的精细定位.

  9. Antinutritional and anticarcinogenic effects of phytic acid and trypsin inhibitor in soybean%大豆中植酸和胰蛋白酶抑制剂的抗营养和抗癌效应

    Institute of Scientific and Technical Information of China (English)

    黄凯; 郑田要; 李聚海; 滕紫; 袁德保

    2008-01-01

    大豆是重要的油料作物和植物蛋白来源,富含植物化学物质,如植酸和胰蛋白酶抑制剂等.传统营养理论认为这些植物化学物质是抗营养因子,然而近来研究显示这些化学成分具有天然抗癌作用.以植酸和STI为例主要介绍了它们的结构、分布、生理活性、抗营养效应及抗癌效应.

  10. Investigation of Carboxylic Acid-Neodymium Conversion Films on Magnesium Alloy

    Science.gov (United States)

    Cui, Xiufang; Liu, Zhe; Lin, Lili; Jin, Guo; Wang, Haidou; Xu, Binshi

    2015-01-01

    The new carboxylic acid-neodymium anhydrous conversion films were successfully prepared and applied on the AZ91D magnesium alloy surface by taking absolute ethyl alcohol as solvent and four kinds of soluble carboxylic acid as activators. The corrosion resistance of the coating was measured by potentiodynamic polarization test in 3.5 wt.% NaCl solution in pH 7.0. The morphology, structure, and constituents of the coating were observed by scanning electron microscope, energy dispersivespectrum, x-ray photoelectron spectrum, and Fourier infrared spectrometer. Results show that corrosion resistance properties of samples coated with four different anhydrous conversion films were improved obviously. The corrosion potential increased, corrosion current density decreased, and polarization resistance increased. Among these four kinds of conversion films the one added with phytic exhibits the best corrosion resistant property. The mechanism of anhydrous-neodymium conversion film formation is also analyzed in this paper. It reveals that the gadolinium conversion coating is mainly composed of stable Nd2O3, MgO, Mg(OH)2, and carboxylate of Nd. And that the sample surface is rich in organic functional groups.

  11. Effect of dehulling, germination and cooking on nutrients, anti-nutrients, fatty acid composition and antioxidant properties in lentil (Lens culinaris).

    Science.gov (United States)

    Pal, R S; Bhartiya, Anuradha; Yadav, Pradhuman; Kant, Lakshmi; Mishra, K K; Aditya, J P; Pattanayak, A

    2017-03-01

    The changes in chemical composition, antioxidant activity and fatty acid composition of lentil flour after dehulling, germination and cooking of seeds were investigated. Dehulling showed no significant effect on protein content, however, protein content decreased in most of the varieties after germination and cooking. Total soluble sugars (TSS) content increased significantly after dehulling (2.0-41.64 %) and cooking (2.08-31.07 %) whereas, germination had no significant effect on TSS content. Total lipids increased significantly after dehulling (21.56-42.86 %) whereas, it decreased significantly after germination (2.97-26.52 %) and cooking (23.05-58.63 %). Cooking was more effective than other methods in reducing trypsin inhibitors (80.51-85.41 %). Dehulling was most effective in reducing tannins (89.46-92.99 %) and phytic acid (52.63-60.00 %) content over raw seed. Myristic, palmitic, stearic, oleic and linoleic acid content decreased while linolenic acid content increased after dehulling. Dehulling, germination and cooking decreased the content of antioxidant metabolite (gallic acid, catechin and quercetin) and also antioxidant activities. Raw samples followed by germinated samples showed the highest concentrations of phytochemicals responsible for antioxidant activity and also the antioxidant capacities. Present study showed germination and cooking would be useful in formulation and development of lentil based functional foods for human health benefits.

  12. Efeito da germinação de soja CV. BR-13 e Paraná sobre ácido fítico, fósforo total e inibidores de tripsina Germination effect of soybean CV. BR-13 and Paraná on phitic acid, total P and trypsin inhibitors levels

    Directory of Open Access Journals (Sweden)

    Mara Lucia Luiz Ribeiro

    1999-01-01

    Full Text Available Este trabalho foi conduzido para avaliar o efeito da germinação sobre os constituintes antinutricionais da soja, ácido fítico, P total e inibidores de tripsina, que interferem na utilização de proteínas e outras substâncias. A diminuição no aproveitamento de nutrientes resulta em inibição de crescimento, hipoglicemia ou danos a tecidos animais. Para os ensaios foram utilizadas as cultivares de soja (Glycine max (L. Merrill BR-13 e Paraná. As sementes foram incubadas em câmara de germinação a 25°C e 100% de umidade, de 0 a 72 horas, com coleta de amostras em intervalos eqüidistantes de seis horas. A cultivar Paraná apresentou teores superiores de ácido fítico, P total e inibidores de tripsina, em relação à cultivar BR-13. As análises de variância e comparações entre médias indicaram que houve aumentos significativos (p The aim of this work was to evaluate the effect of germination on the antinutritional constituents of soybean, phytic acid, total P and trypsin inhibitors that interfere with the utilization of proteins and other substances. The decrease of the nutrient utilization results in growth inhibition, hypoglycemia or damage to animal tissues. Seeds of soybean (Glycine max (L. Merrill cultivars BR-13 and Paraná were incubated in germination chamber at 25°C and 100% of humidity, from 0 to 72 hours, and samples were collected in intervals of six hours. The Paraná cultivar presented higher amount of phytic acid, total phosphorus and trypsin inhibitors, than the BR-13 cultivar. The analysis of variance and comparison among the means indicated that, in 72 hours of germination, there was a significant increase (p<0.05 on the amount of total phosphorus (8.5% and 3.6% for BR-13 and Paraná cultivars, respectively and on the level of trypsin inhibitors (22.05% and 17.10% for BR-13 and Paraná cultivars, respectively while the amount of phytic acid decreased 15.96% and 9.46% for BR-13 and Paraná cultivars

  13. Valproic Acid

    Science.gov (United States)

    ... acid is in a class of medications called anticonvulsants. It works by increasing the amount of a ... older (about 1 in 500 people) who took anticonvulsants such as valproic acid to treat various conditions ...

  14. Amino acids

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/article/002222.htm Amino acids To use the sharing features on this page, please enable JavaScript. Amino acids are organic compounds that combine to form proteins . ...

  15. Obeticholic Acid

    Science.gov (United States)

    Obeticholic acid is used alone or in combination with ursodiol (Actigall, Urso) to treat primary biliary cholangitis (PBC; a ... were not treated successfully with ursodiol alone. Obeticholic acid is in a class of medications called farnesoid ...

  16. Ascorbic Acid

    Science.gov (United States)

    Ascorbic acid is used to prevent and treat scurvy, a disease caused by a lack of vitamin C in ... Ascorbic acid comes in extended-release (long-acting) capsules and tablets, lozenges, syrup, chewable tablets, and liquid drops to ...

  17. Mefenamic Acid

    Science.gov (United States)

    Mefenamic acid is used to relieve mild to moderate pain, including menstrual pain (pain that happens before or during a menstrual period). Mefenamic acid is in a class of medications called NSAIDs. ...

  18. Acid mucopolysaccharides

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/article/003368.htm Acid mucopolysaccharides To use the sharing features on this page, please enable JavaScript. Acid mucopolysaccharides is a test that measures the amount ...

  19. Ethacrynic Acid

    Science.gov (United States)

    Ethacrynic acid, a 'water pill,' is used to treat swelling and fluid retention caused by various medical problems. It ... Ethacrynic acid comes as a tablet to take by mouth. It is usually taken once or twice a day ...

  20. Aminocaproic Acid

    Science.gov (United States)

    Aminocaproic acid is used to control bleeding that occurs when blood clots are broken down too quickly. This type ... the baby is ready to be born). Aminocaproic acid is also used to control bleeding in the ...

  1. Aspartic acid

    Science.gov (United States)

    ... body work. It plays a role in: Hormone production and release Normal nervous system function Plant sources of aspartic acid include: avocado, asparagus, and molasses. Animal sources of aspartic acid include: ...

  2. Fatty acids - trans fatty acids

    Science.gov (United States)

    The data supporting a negative effect of dietary trans fatty acids on cardiovascular disease risk is consistent. The primary dietary sources of trans fatty acids include partially hydrogenated fat and rudiment fat. The adverse effect of trans fatty acids on plasma lipoprotein profiles is consisten...

  3. Phytate degrading activities of lactic acid bacteria isolated from traditional fermented food

    Science.gov (United States)

    Damayanti, Ema; Ratisiwi, Febiyani Ndaru; Istiqomah, Lusty; Sembiring, Langkah; Febrisiantosa, Andi

    2017-03-01

    The objective of this study was to determine the potential of LAB with phytate degrading activity from fermented traditional food grain-based and legume-based. Lactic acid bacteria were isolated from different sources of traditional fermented food from Gunungkidul Yogyakarta Indonesia such as gembus tempeh (tofu waste), soybean tempeh, lamtoro tempeh (Leucaena bean) and kara tempeh. Isolation of LAB was performed using Total Plate Count (TPC) on de Man Rogosa Sharpe Agar (MRSA) medium supplemented with CaCO3. They were screened for their ability to degrade myo-inositol hexaphosphate or IP6 by using qualitative streak platemethod with modified de Man Rogosa-MorpholinoPropanesulfonic Acid Sharpe (MRS-MOPS) medium contained sodium salt of phytic acid as substrate and cobalt chloride staining (plate assay) method. The selected isolates were further assayed for phytase activities using quantitative method with spectrophotometer and the two selected isolates growth were optimized. Furthermore, thhe isolates that shown the highest phytase activity was characterized and identified using API 50 CH kitand 16S rRNA gene sequencing. The results showed that there were 18 LAB isolates obtained from samplesand 13 isolates were able to degrade sodium phytate based on qualitative screening. According to quantitative assay, the highest phytate degrading activities were found in TG-2(23.562 U/mL) and TG-1 (19.641 U/mL) isolated from gembus tempeh. The phytate activity of TG-2 was optimum at 37 °C with agitation, while the phytate activity of TG-1 was optimum at 45 °C without agitation. Characterization and identification of TG-2 isolate with the highest phytate degrading activity using API 50 CH and 16S rRNA showed that TG-2had homology with Lactobacillus fermentum. It could be concluded that LAB from from fermented traditional food grain-based and legume-based produced the extracellular phytase. Keywords: lactic acid bacteria, tempeh, phytatedegrading activity

  4. Acid-doped polymer nanofiber framework: Three-dimensional proton conductive network for high-performance fuel cells

    Science.gov (United States)

    Tanaka, Manabu; Takeda, Yasushi; Wakiya, Takeru; Wakamoto, Yuta; Harigaya, Kaori; Ito, Tatsunori; Tarao, Takashi; Kawakami, Hiroyoshi

    2017-02-01

    High-performance polymer electrolyte membranes (PEMs) with excellent proton conductivity, gas barrier property, and membrane stability are desired for future fuel cells. Here we report the development of PEMs based on our proposed new concept "Nanofiber Framework (NfF)." The NfF composite membranes composed of phytic acid-doped polybenzimidazole nanofibers (PBINf) and Nafion matrix show higher proton conductivity than the recast-Nafion membrane without nanofibers. A series of analyses reveal the formation of three-dimensional network nanostructures to conduct protons and water effectively through acid-condensed layers at the interface of PBINf and Nafion matrix. In addition, the NfF composite membrane achieves high gas barrier property and distinguished membrane stability. The fuel cell performance by the NfF composite membrane, which enables ultra-thin membranes with their thickness less than 5 μm, is superior to that by the recast-Nafion membrane, especially at low relative humidity. Such NfF-based high-performance PEM will be accomplished not only by the Nafion matrix used in this study but also by other polymer electrolyte matrices for future PEFCs.

  5. Standardized ileal digestibility of proteins and amino acids in sesame expeller and soya bean meal in weaning piglets.

    Science.gov (United States)

    Aguilera, A; Reis de Souza, T C; Mariscal-Landín, G; Escobar, K; Montaño, S; Bernal, M G

    2015-08-01

    Apparent ileal digestibility (AID) of diets containing sesame expeller (SE) and soya bean meal (SBM) was determined using 15 piglets (Genetiporc(®)), weaned at 17 ± 0.4 days with average body weight of 6.4 ± 0.7 kg (Fertilis 20 × G Performance, Genetiporc(®), PIC México, Querétaro, México). Piglets were randomly assigned to three treatments: (i) a reference diet with casein as the sole protein source; (ii) a mixed diet of casein-SE; and (iii) a mixed diet of casein-SBM. The chemical composition of SE and SBM was determined, and AID and standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AAs) were determined for each protein source. SE contained greater quantities of ether extract, neutral detergent fibre, phytic acid, methionine and arginine than SBM. Lysine and proline contents and trypsin inhibitor activity were higher in SBM than in SE. The AID and SID of CP and AA (except for lysine and proline) were similar in SE and SBM. The AID of lysine and proline was higher in SBM than in SE (p < 0.05), and the SID of proline was higher in SE than in SBM (p < 0.05). These findings indicate that SE is an appropriate alternative protein source for early weaned pigs. Journal of Animal Physiology and Animal Nutrition © 2014 Blackwell Verlag GmbH.

  6. Folic Acid

    Science.gov (United States)

    ... damage. 10 Do I need folic acid after menopause? Yes. Women who have gone through menopause still need 400 micrograms of folic acid every ... United States: 2003–2006 . American Journal of Clinical Nutrition; 91(1): 231–237. Hamner, H.C., Cogswell, ...

  7. Folic acid

    Science.gov (United States)

    ... taking a specific nutritional supplement, containing vitamin B3 (nicotinamide), a compound isolated from grains (azelaic acid), zinc, ... lung cancer in most people. A type of skin cancer called melanoma. Limited research suggests that taking ...

  8. Folic Acid

    Science.gov (United States)

    ... B-complex vitamin needed by the body to manufacture red blood cells. A deficiency of this vitamin ... prepared from dried yeast, fruit, and fresh leafy green vegetables to increase the folic acid in your ...

  9. Ibotenic acid and thioibotenic acid

    DEFF Research Database (Denmark)

    Hermit, Mette B; Greenwood, Jeremy R; Nielsen, Birgitte

    2004-01-01

    with the conformations preferred by the ligands upon docking to mGlu1 and models of the other mGlu subtypes, we propose that unlike other subtypes, group III mGlu receptor binding sites require a ligand conformation at an energy level which is prohibitively expensive for ibotenic acid, but not for thioibotenic acid....... These studies demonstrate how subtle differences in chemical structures can result in profound differences in pharmacological activity....

  10. Methods for analysis of the distribution and speciation of iron and zinc in the cereal grain

    DEFF Research Database (Denmark)

    Hansen, Thomas Hesselhøj

    micronutrients in cereal grain primarily depends on two main components; phytic acid and proteins. Most of the phosphorus (P) found in the grain is bound in phytic acid and most sulphur (S) is incorporated into the amino acids cysteine and methionine that are part of different proteins. Co...

  11. Acid Rain

    Science.gov (United States)

    Bricker, Owen P.; Rice, Karen C.; Dietrich, W.E.; Sposito, Garrison

    1997-01-01

    Acid deposition, or acid rain as it is more commonly referred to, has become a widely publicized environmental issue in the U.S. over the past decade. The term usually conjures up images of fish kills, dying forests, "dead" lakes, and damage to monuments and other historic artifacts. The primary cause of acid deposition is emission of S02 and NOx to the atmosphere during the combustion of fossil fuels. Oxidation of these compounds in the atmosphere forms strong acids - H2SO4 and HNO3 - which are returned to the Earth in rain, snow, fog, cloud water, and as dry deposition.Although acid deposition has only recently been recognized as an environmental problem in the U.S., it is not a new phenomenon (Cogbill & Likens 1974). As early as the middle of the 17th century in England, the deleterious effects of industrial emissions on plants, animals, and humans, and the atmospheric transport of pollutants between England and France had become issues of concern (Evelyn 1661, Graunt 1662). It is interesting that well over three hundred years ago in England, recommendations were made to move industry outside of towns and build higher chimneys to spread the pollution into "distant parts." Increasing the height of smokestacks has helped alleviate local problems, but has exacerbated others. In the U.S. the height of the tallest smokestack has more than doubled, and the average height of smokestacks has tripled since the 1950s (Patrick et al 1981). This trend occurred in most industrialized nations during the 20th century and has had the effect of transforming acid rain from a local urban problem into a problem of global scale.

  12. Perfluorooctanoic acid

    NARCIS (Netherlands)

    de Voogt, P.; Wexler, P.

    2014-01-01

    Perfluorooctanoic acid (PFOA, 335-67-1) is used in fluoropolymer production and firefighting foams and persists in the environment. Human exposure to PFOA is mostly through the diet. PFOA primarily affects the liver and can cause developmental and reproductive toxic effects in test animals.

  13. Hydroxycarboxylic acids and salts

    Energy Technology Data Exchange (ETDEWEB)

    Kiely, Donald E; Hash, Kirk R; Kramer-Presta, Kylie; Smith, Tyler N

    2015-02-24

    Compositions which inhibit corrosion and alter the physical properties of concrete (admixtures) are prepared from salt mixtures of hydroxycarboxylic acids, carboxylic acids, and nitric acid. The salt mixtures are prepared by neutralizing acid product mixtures from the oxidation of polyols using nitric acid and oxygen as the oxidizing agents. Nitric acid is removed from the hydroxycarboxylic acids by evaporation and diffusion dialysis.

  14. Dephytinisation with Intrinsic Wheat Phytase and Iron Dephytinisation with Intrinsic Wheat Phytase and Iron Fonio (Digitaria exilis) Meals in West African Women

    NARCIS (Netherlands)

    Koreissi, Y.; Fanou-Fogny, N.M.L.; Moretti, D.; Schuth, S.; Dossa, R.A.M.; Egli, I.; Zimmerman, M.B.; Brouwer, I.D.

    2013-01-01

    Low iron and high phytic acid content make fonio based meals a poor source of bioavailable iron. Phytic acid degradation in fonio porridge using whole grain cereals as phytase source and effect on iron bioavailability when added to iron fortified fonio meals were investigated. Grains, nuts and seeds

  15. Phytases for improved iron absorption

    DEFF Research Database (Denmark)

    Nielsen, Anne Veller Friis; Meyer, Anne S.

    2016-01-01

    Phytase enzymes present an alternative to iron supplements, because they have been shown to improve iron absorption by means of catalysing the degradation of a potent iron absorption inhibitor: phytic acid. Phytic acid is a hexaphosphate of inositol and is particularly prevalent in cereal grains...

  16. The ABCs of low-phytate crops

    Science.gov (United States)

    The maize (Zea mays L.) low phytic acid 1 gene, initially defined genetically and mapped by USDA-ARS research, was cloned by scientists at Dupont/Pioneer. Phytic acid (inositol hexaphosphate) represents the major form of phosphorus in seeds and is important to the management of phosphorus in livesto...

  17. Evaluation and Development of Low Phytate Crops

    Science.gov (United States)

    Phytic acid (myo-inositol 1,2,3,4,5,6 hexakisphosphate) is the most abundant form of phosphorus (P) in plant seeds, representing ~70%±10% of seed total P. Non-ruminant, mono-gastric animals (poultry, swine, fish), typically do not efficiently digest and utilize seed-derived phytic acid P. This creat...

  18. Approaches and Challenges to Engineering Seed Phytate and Total Phosphorus

    Science.gov (United States)

    About 75% of seed total phosphorus (P) is found in a single compound, phytic acid (myo-inositol-1,2,3,4,5,6-hexakisphosphate or InsP6). Phytic acid is not efficiently utilized by monogastric animals (poultry, swine, fish), which creates phosphorus management and environmental impact problems in anim...

  19. Conversion of seed phytate to utilizable phosphorus in soybean seeds expressing a bacterial phytase

    Science.gov (United States)

    Phytic acid contains the major part of the phosphorus in the soybean, chelates divalent cations, and is not digested by monogastric animals. Untreated soymeal does not provide monogastrics sufficient phosphorus and minerals, and phytic acid in the waste stream leads to phosphorus run-off. We gener...

  20. Calcium - Function and effects

    NARCIS (Netherlands)

    Liang, Jianfen; He, Yifan; Gao, Qian; Wang, Xuan; Nout, M.J.R.

    2016-01-01

    Rice is the primary food source for more than half of the world population. Levels of calcium contents and inhibitor - phytic acid are summarized in this chapter. Phytic acid has a very strong chelating ability and it is the main inhibit factor for calcium in rice products. Calcium contents in br

  1. Hydrofluoric acid poisoning

    Science.gov (United States)

    Fluorhydric acid ... stomach, or intestine have holes (perforations) from the acid. ... Hydrofluoric acid is especially dangerous. The most common accidents involving hydrofluoric acid cause severe burns on the skin ...

  2. Dehydroabietic acid

    Directory of Open Access Journals (Sweden)

    Xiao-Ping Rao

    2009-10-01

    Full Text Available The title compound [systematic name: (1R,4aS,10aR-7-isopropyl-1,4a-dimethyl-1,2,3,4,4a,9,10,10a-octahydrophenanthrene-1-carboxylic acid], C20H28O2, has been isolated from disproportionated rosin which is obtained by isomerizing gum rosin with a Pd-C catalyst.. Two crystallographically independent molecules exist in the asymmetric unit. In each molecule, there are three six-membered rings, which adopt planar, half-chair and chair conformations. The two cyclohexane rings form a trans ring junction with the two methyl groups in axial positions. The crystal structure is stabilized by intermolecular O—H...O hydrogen bonds.

  3. Amino acids in the sedimentary humic and fulvic acids

    Digital Repository Service at National Institute of Oceanography (India)

    Sardessai, S.

    to the coastal sedimentary humic acids implying higher association of amino acids with the carbonaceous and fine grained sedimentary humic acids. Both the humic and fulvic acids are composed of neutral, acidic, basic, aromatic and sulphur containing amino acids....

  4. [Teichoic acids from lactic acid bacteria].

    Science.gov (United States)

    Livins'ka, O P; Harmasheva, I L; Kovalenko, N K

    2012-01-01

    The current view of the structural diversity of teichoic acids and their involvement in the biological activity of lactobacilli has been reviewed. The mechanisms of effects of probiotic lactic acid bacteria, in particular adhesive and immunostimulating functions have been described. The prospects of the use of structure data of teichoic acid in the assessment of intraspecific diversity of lactic acid bacteria have been also reflected.

  5. Plasma amino acids

    Science.gov (United States)

    Amino acids blood test ... types of methods used to determine the individual amino acid levels in the blood. ... test is done to measure the level of amino acids in the blood. An increased level of a ...

  6. Uric acid test (image)

    Science.gov (United States)

    Uric acid urine test is performed to check for the amount of uric acid in urine. Urine is collected over a 24 ... testing. The most common reason for measuring uric acid levels is in the diagnosis or treatment of ...

  7. POLYELEOSTEARIC ACID VESICLES

    Institute of Scientific and Technical Information of China (English)

    LI Zichen; XIE Ximng; FAN Qinghua; FANG Yifei

    1992-01-01

    α-Eleostearic acid and β-eleostearic acid formed vesicles in aqueous medium when an ethanol solutionofeleostearic acid was injected rapidly into a vigorously vortexed aqueous phase. Formation of the vesicles was demonstrated by electron microscopic observation and bromothymol blue encapsulation experiments. Polymerizations of the eleostearic acids in the formed vesicles carried out by UV irradiation produced poly-α-eleostearic acid and poly-β-eleostearic acid vesicles.

  8. Acid distribution in phosphoric acid fuel cells

    Energy Technology Data Exchange (ETDEWEB)

    Okae, I.; Seya, A.; Umemoto, M. [Fuji Electric Co., Ltd., Chiba (Japan)

    1996-12-31

    Electrolyte acid distribution among each component of a cell is determined by capillary force when the cell is not in operation, but the distribution under the current load conditions had not been clear so far. Since the loss of electrolyte acid during operation is inevitable, it is necessary to store enough amount of acid in every cell. But it must be under the level of which the acid disturbs the diffusion of reactive gases. Accordingly to know the actual acid distribution during operation in a cell is very important. In this report, we carried out experiments to clarify the distribution using small single cells.

  9. Fósforo total, fósforo fítico y actividad fitásica en los frutos de árboles forrajeros de los Llanos Centrales de Venezuela Total phosphorus, phytic phosphorus and phytase activity in the fruits of forage trees from the Central Plains, Venezuela

    Directory of Open Access Journals (Sweden)

    P Pizzani

    2009-06-01

    Full Text Available Con el objetivo de conocer la concentración de fósforo total (PT, fósforo fítico (PF y la actividad fitásica intrínseca (AFi de algunos frutos de árboles de interés forrajero de los Llanos Centrales de Venezuela: samán (Pithecellobium saman, carocaro (Enterolobium cyclocarpum, cují hediondo (Acacia macracantha, cañafistolillo (Cassia emarginata, caruto (Genipa americana, dividive (Caesalpinia coriaria, granadillo (Caesalpinia granadillo, guamacho (Pereskia guamacho, tiamo (Acacia glomerosa, guácimo (Guazuma ulmifolia, merecure (Licania pyrifolia y cují blanco (Prosopis juliflora, se realizó un ensayo mediante un diseño totalmente aleatorizado y diez réplicas. Los resultados indicaron valores de PT entre 0,08 y 0,38% y los contenidos de PF oscilaron entre 0,02 y 0,28%; ambas variables presentaron diferencias significativas entre especies (PWith the objective of learning the total phosphorus (TP and phytic phosphorus (PP concentrations and the intrinsic phytase activity (PAi in some fruits of forage trees from the Central Plains, Venezuela: Pithecellobium saman, Enterolobium cyclocarpum, Acacia macracantha, Cassia emarginata, Genipa americana, Caesalpinia coriaria, Caesalpinia granadillo, Pereskia guamacho, Acacia glomerosa, Guazuma ulmifolia, Licania pyrifolia and Prosopis juliflora, a trial was conducted by means of a completely randomized design and ten replications. The results indicated TP values between 0,08 and 0,38% and the PP contents oscillated between 0,02 and 0,28%; both variables showed significant differences among species (P<0,05. P. guamacho had the highest TP and PP concentration. The PAi of 91,7% of the fruits was higher than 100 Ukg-1; the rest showed values between 94 and 95 Ukg-1. The PP content was highly correlated to TP concentration (r = 0,97; P<0,01. However, the PAi did not show significant relationship to the TP and PP values. Only L. pyrifolia recorded a lower value (0,08%. The PP content, as percentage

  10. Genetic variation for seed phosphorus and yield traits in Indian sorghum landraces and varieties

    Institute of Scientific and Technical Information of China (English)

    Ashok Badigannavar; G. Girish; T.R. Ganapathi

    2015-01-01

    Phytic acid is the major storage form of phosphorus in cereals. It binds with nutritionally important metals and affects mineral bioavailability. The present study analyzed phytic acid, inorganic phosphorus (IP) content, seed weight, and grain yield in 98 sorghum landraces and varieties grown in two environments to evaluate genotypic and environmental effects and to determine trait stability. Genotypic effects and genotype × interaction were significant for phytic acid concentration and yield components. A promising landrace, Malkhed-1, had the lowest phytic acid (0.015 mg g−1) concentration, with a higher yield (70.02 g plant−1), than the check variety M-35-1 in both environments. Similarly, among the varieties, Phule Maulee showed the lowest phytic acid (0.07 mg g−1) and a higher grain yield of 53.15 g plant−1 in both environments. Phytic acid and IP were negatively correlated (r=−0.34), whereas grain yield and seed weight were positively correlated (r=0.20). Cluster analysis based on seed phosphorus traits and yield components identified five and six clusters, respectively. Genotypes containing low phytic acid with high yield identified in this study would be helpful for increasing the bioavailability of mineral nutrients.

  11. Genetic variation for seed phosphorus and yield traits in Indian sorghum landraces and varieties

    Institute of Scientific and Technical Information of China (English)

    Ashok; Badigannavar; G.Girish; T.R.Ganapathi

    2015-01-01

    Phytic acid is the major storage form of phosphorus in cereals. It binds with nutritionally important metals and affects mineral bioavailability. The present study analyzed phytic acid, inorganic phosphorus(IP) content, seed weight, and grain yield in 98 sorghum landraces and varieties grown in two environments to evaluate genotypic and environmental effects and to determine trait stability. Genotypic effects and genotype × interaction were significant for phytic acid concentration and yield components. A promising landrace, Malkhed-1, had the lowest phytic acid(0.015 mg g-1)concentration, with a higher yield(70.02 g plant-1), than the check variety M-35-1 in both environments. Similarly, among the varieties, Phule Maulee showed the lowest phytic acid(0.07 mg g-1) and a higher grain yield of 53.15 g plant-1in both environments. Phytic acid and IP were negatively correlated(r =- 0.34), whereas grain yield and seed weight were positively correlated(r = 0.20). Cluster analysis based on seed phosphorus traits and yield components identified five and six clusters, respectively. Genotypes containing low phytic acid with high yield identified in this study would be helpful for increasing the bioavailability of mineral nutrients.

  12. Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre (BARC, Mumbai 400085, India

    Directory of Open Access Journals (Sweden)

    Ashok Badigannavar

    2015-08-01

    Full Text Available Phytic acid is the major storage form of phosphorus in cereals. It binds with nutritionally important metals and affects mineral bioavailability. The present study analyzed phytic acid, inorganic phosphorus (IP content, seed weight, and grain yield in 98 sorghum landraces and varieties grown in two environments to evaluate genotypic and environmental effects and to determine trait stability. Genotypic effects and genotype × interaction were significant for phytic acid concentration and yield components. A promising landrace, Malkhed-1, had the lowest phytic acid (0.015 mg g− 1 concentration, with a higher yield (70.02 g plant− 1, than the check variety M-35-1 in both environments. Similarly, among the varieties, Phule Maulee showed the lowest phytic acid (0.07 mg g− 1 and a higher grain yield of 53.15 g plant− 1 in both environments. Phytic acid and IP were negatively correlated (r = − 0.34, whereas grain yield and seed weight were positively correlated (r = 0.20. Cluster analysis based on seed phosphorus traits and yield components identified five and six clusters, respectively. Genotypes containing low phytic acid with high yield identified in this study would be helpful for increasing the bioavailability of mineral nutrients.

  13. Phytate in foods and significance for humans: food sources, intake, processing, bioavailability, protective role and analysis.

    Science.gov (United States)

    Schlemmer, Ulrich; Frølich, Wenche; Prieto, Rafel M; Grases, Felix

    2009-09-01

    The article gives an overview of phytic acid in food and of its significance for human nutrition. It summarises phytate sources in foods and discusses problems of phytic acid/phytate contents of food tables. Data on phytic acid intake are evaluated and daily phytic acid intake depending on food habits is assessed. Degradation of phytate during gastro-intestinal passage is summarised, the mechanism of phytate interacting with minerals and trace elements in the gastro-intestinal chyme described and the pathway of inositol phosphate hydrolysis in the gut presented. The present knowledge of phytate absorption is summarised and discussed. Effects of phytate on mineral and trace element bioavailability are reported and phytate degradation during processing and storage is described. Beneficial activities of dietary phytate such as its effects on calcification and kidney stone formation and on lowering blood glucose and lipids are reported. The antioxidative property of phytic acid and its potentional anticancerogenic activities are briefly surveyed. Development of the analysis of phytic acid and other inositol phosphates is described, problems of inositol phosphate determination and detection discussed and the need for standardisation of phytic acid analysis in foods argued.

  14. Gas-phase Acidities of Aspartic Acid, Glutamic Acid, and their Amino Acid Amides.

    Energy Technology Data Exchange (ETDEWEB)

    Li, Zhong; Matus, Myrna H; Velazquez, Hector A; Dixon, David A; Cassady, Carolyn J

    2007-02-14

    Gas-phase acidities (GA or ΔGacid) for the two most acidic common amino acids, aspartic acid and glutamic acid, have been determined for the first time. Because of the amide linkage’s importance in peptides and as an aid in studying side chain versus main chain deprotonation, aspartic acid amide and glutamic acid amide were also studied. Experimental GA values were measured by proton transfer reactions in an electrospray ionization/Fourier transform ion cyclotron resonance mass spectrometer. Calculated GAs were obtained by density functional and molecular orbital theory approaches. The best agreement with experiment was found at the G3MP2 level; the MP2/CBS and B3LYP/aug-cc-pVDZ results are 3–4 kcal/mol more acidic than the G3MP2 results. Experiment shows that aspartic acid is more acidic than glutamic acid by ca. 3 kcal/mol whereas the G3MP2 results show a smaller acidity difference of 0.2 kcal/mol. Similarly, aspartic acid amide is experimentally observed to be ca. 2 kcal/mol more acidic than glutamic acid amide whereas the G3MP2 results show a correspondingly smaller energy difference of 0.7 kcal/mol. The computational results clearly show that the anions are all ring-like structures with strong hydrogen bonds between the OH or NH2 groups and the CO2- group from which the proton is removed. The two amino acids are main-chain deprotonated. In addition, use of the COSMO model for the prediction of the free energy differences in aqueous solution gave values in excellent agreement with the most recent experimental values for pKa. Glutamic acid is predicted to be more acidic than aspartic acid in aqueous solution due to differential solvation effects.

  15. Gas-phase acidities of aspartic acid, glutamic acid, and their amino acid amides

    Science.gov (United States)

    Li, Zhong; Matus, Myrna H.; Velazquez, Hector Adam; Dixon, David A.; Cassady, Carolyn J.

    2007-09-01

    Gas-phase acidities (GA or [Delta]Gacid) for the two most acidic common amino acids, aspartic acid and glutamic acid, have been determined for the first time. Because of the amide linkage's importance in peptides and as an aid in studying side chain versus main chain deprotonation, aspartic acid amide and glutamic acid amide were also studied. Experimental GA values were measured by proton transfer reactions in an electrospray ionization/Fourier transform ion cyclotron resonance mass spectrometer. Calculated GAs were obtained by density functional and molecular orbital theory approaches. The best agreement with experiment was found at the G3MP2 level; the MP2/CBS and B3LYP/aug-cc-pVDZ results are 3-4 kcal/mol more acidic than the G3MP2 results. Experiment shows that aspartic acid is more acidic than glutamic acid by ca. 3 kcal/mol whereas the G3MP2 results show a smaller acidity difference of 0.2 kcal/mol. Similarly, aspartic acid amide is experimentally observed to be ca. 2 kcal/mol more acidic than glutamic acid amide whereas the G3MP2 results show a correspondingly smaller energy difference of 0.7 kcal/mol. The computational results clearly show that the anions are all ring-like structures with strong hydrogen bonds between the OH or NH2 groups and the CO2- group from which the proton is removed. The two amino acids are main-chain deprotonated. In addition, use of the COSMO model for the prediction of the free energy differences in aqueous solution gave values in excellent agreement with the most recent experimental values for pKa. Glutamic acid is predicted to be more acidic than aspartic acid in aqueous solution due to differential solvation effects.

  16. Toxicity of adipic acid.

    Science.gov (United States)

    Kennedy, Gerald L

    2002-05-01

    Adipic acid has very low acute toxicity in rats with an LD50 > 5000 mg/kg. Adipic acid produced mild to no skin irritation on intact guinea pig skin as a 50% concentration in propylene glycol; it was not a skin sensitizer. Adipic acid caused mild conjunctival irritation in washed rabbit eyes; in unwashed rabbit eyes, there was mild conjunctival irritation, minimal iritis, but no corneal effects. Adipic acid dust may irritate the mucous membranes of the lungs and nose. In a 2-year feeding study, rats fed adipic acid at concentrations up to 5% in the diet exhibited only weight loss. Adipic acid is not genetically active in a wide variety of assay systems. Adipic acid caused no developmental toxicity in mice, rats, rabbits, or hamsters when administered orally. Adipic acid is partially metabolized in humans; the balance is eliminated unchanged in the urine. Adipic acid is slightly to moderately toxic to fish, daphnia, and algae in acute tests.

  17. Acid Thunder: Acid Rain and Ancient Mesoamerica

    Science.gov (United States)

    Kahl, Jonathan D. W.; Berg, Craig A.

    2006-01-01

    Much of Mesoamerica's rich cultural heritage is slowly eroding because of acid rain. Just as water dissolves an Alka-Seltzer tablet, acid rain erodes the limestone surfaces of Mexican archaeological sites at a rate of about one-half millimeter per century (Bravo et al. 2003). A half-millimeter may not seem like much, but at this pace, a few…

  18. Acid Thunder: Acid Rain and Ancient Mesoamerica

    Science.gov (United States)

    Kahl, Jonathan D. W.; Berg, Craig A.

    2006-01-01

    Much of Mesoamerica's rich cultural heritage is slowly eroding because of acid rain. Just as water dissolves an Alka-Seltzer tablet, acid rain erodes the limestone surfaces of Mexican archaeological sites at a rate of about one-half millimeter per century (Bravo et al. 2003). A half-millimeter may not seem like much, but at this pace, a few…

  19. Omega-3 Fatty Acids

    Science.gov (United States)

    Omega-3 fatty acids are used together with lifestyle changes (diet, weight-loss, exercise) to reduce the ... the blood in people with very high triglycerides. Omega-3 fatty acids are in a class of ...

  20. Omega-6 Fatty Acids

    Science.gov (United States)

    Omega-6 fatty acids are types of fats. Some types are found in vegetable oils, including corn, evening primrose seed, safflower, and soybean oils. Other types of omega-6 fatty acids are found in black currant ...

  1. Lactic acid test

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/article/003507.htm Lactic acid test To use the sharing features on this page, please enable JavaScript. Lactic acid is mainly produced in muscle cells and red ...

  2. Catalytic Synthesis Lactobionic Acid

    Directory of Open Access Journals (Sweden)

    V.G. Borodina

    2014-07-01

    Full Text Available Gold nanoparticles are obtained, characterized and deposited on the carrier. Conducted catalytic synthesis of lactobionic acid from lactose. Received lactobionic acid identify on the IR spectrum.

  3. Amino Acid Metabolism Disorders

    Science.gov (United States)

    ... this process. One group of these disorders is amino acid metabolism disorders. They include phenylketonuria (PKU) and maple syrup urine disease. Amino acids are "building blocks" that join together to form ...

  4. Facts about Folic Acid

    Science.gov (United States)

    ... Partners About Us Information For… Media Policy Makers Facts About Folic Acid Language: English (US) Español ( ... a woman needs 400 micrograms (mcg) every day. Facts About Folic Acid Download and print this fact ...

  5. Azelaic Acid Topical

    Science.gov (United States)

    Azelaic acid gel and foam is used to clear the bumps, lesions, and swelling caused by rosacea (a skin ... redness, flushing, and pimples on the face). Azelaic acid cream is used to treat the pimples and ...

  6. Folic Acid Quiz

    Science.gov (United States)

    ... About Us Information For… Media Policy Makers Folic Acid Quiz Language: English (US) Español (Spanish) Recommend ... button beside the question. Good Luck! 1. Folic acid is: A a B vitamin B a form ...

  7. 直酸酶在仔猪生产中的应用

    Institute of Scientific and Technical Information of China (English)

    张闯; 邹小峰; 喻洪湛; 高荣玲

    2011-01-01

    磷是畜禽营养物质中功能最多的营养素之一。植酸(Phytic acid)和植酸盐(Phytic acid salt)是谷物饲料中磷的主要存在形式。由于单胃动物(猪、鸡等)的消化道中缺乏水解植酸及其盐类的植酸酶,所以不能充分消化利用植酸(Phytic acid)和植酸盐中的磷,必须在饲料中添加无机磷酸盐。因此饲料中添加植酸酶是解决磷吸收不足,促进仔猪生长的一个重要的方法。%Phosphorus is one of the functional nutriment in the livestock and poultry nutrients. Phytic acid and phytic acid salt is major forms of phosphorus in grain feed. Single stomach animals'gastrointestina is lack of phytic acid hydrolysis and phytic acid enz

  8. Immunoglobulin and fatty acids

    DEFF Research Database (Denmark)

    2009-01-01

    The present invention relates to a composition comprising 0.1-10 w/w % immunoglobulin (Ig), 4-14 w/w % saturated fatty acids, 4-14 w/w % mono-unsaturated fatty acids and 0-5 w/w % poly-unsaturated fatty acids, wherein the weight percentages are based on the content of dry matter in the composition...

  9. The Acid Rain Reader.

    Science.gov (United States)

    Stubbs, Harriett S.; And Others

    A topic which is often not sufficiently dealt with in elementary school textbooks is acid rain. This student text is designed to supplement classroom materials on the topic. Discussed are: (1) "Rain"; (2) "Water Cycle"; (3) "Fossil Fuels"; (4) "Air Pollution"; (5) "Superstacks"; (6) "Acid/Neutral/Bases"; (7) "pH Scale"; (8) "Acid Rain"; (9)…

  10. Stomach acid test

    Science.gov (United States)

    Gastric acid secretion test ... of the cells in the stomach to release acid. The stomach contents are then removed and analyzed. ... 3.5). These numbers are converted to actual acid production in units of milliequivalents per hour (mEq/ ...

  11. Acid Lipase Disease

    Science.gov (United States)

    ... Page You are here Home » Disorders » All Disorders Acid Lipase Disease Information Page Acid Lipase Disease Information Page What research is being ... research to understand lipid storage diseases such as acid lipase deficiency. Additional research studies hope to identify ...

  12. Acid Rain Study Guide.

    Science.gov (United States)

    Hunger, Carolyn; And Others

    Acid rain is a complex, worldwide environmental problem. This study guide is intended to aid teachers of grades 4-12 to help their students understand what acid rain is, why it is a problem, and what possible solutions exist. The document contains specific sections on: (1) the various terms used in conjunction with acid rain (such as acid…

  13. The Acid Rain Reader.

    Science.gov (United States)

    Stubbs, Harriett S.; And Others

    A topic which is often not sufficiently dealt with in elementary school textbooks is acid rain. This student text is designed to supplement classroom materials on the topic. Discussed are: (1) "Rain"; (2) "Water Cycle"; (3) "Fossil Fuels"; (4) "Air Pollution"; (5) "Superstacks"; (6) "Acid/Neutral/Bases"; (7) "pH Scale"; (8) "Acid Rain"; (9)…

  14. 大豆MIPS1基因的定位及其低植酸突变性状CAPS标记的开发%Mapping of MIPS1 and Development of CAPS Marker for Low Phytic Acid Mutation in Soybean

    Institute of Scientific and Technical Information of China (English)

    袁凤杰; 姜莹; 朱申龙; 李百权; 付旭军; 朱丹华; 董德坤; 舒庆尧

    2010-01-01

    [目的]定位大豆肌醇-3-磷酸合成酶基因MIPS1,丰富该基因的遗传信息;开发出该基因的特异性分子标记,用于大豆突变体Gm-1pa-TW-1低植酸(LPA)突变性状的辅助选择.[方法]应用公布的大豆基因组数据库和生物信息学分析确定MIPS1候选染色体,利用分离群体中微卫星标记与LPA性状的共分离,定位突变基因MIPS1:根据MIPS1 LPA突变性质和同源基因的序列,开发CAPS分子标记.[结果]大豆中有4个MIPS基因,其中MIPS1被定位在染色体18上,为注释为Glyma18g02210的大豆基因,其DNA起始序列为1 364 774 bp,位于SSR标记Satt570(3 162 690 bp)和Satt115(10 422 881 bp)之上,遗传距高分别为5.5和15.2 cM;开发了MIPS1特异性共显性CAPS标记,与LPA性状完全共分离.[结论]本文首次确定了MIPS1所在染色体及其位置,丰富了该基因的遗传信息,开发的CAPS标记可用于Gm-1pa-TW-1 LPA性状的分子标记辅助选择.

  15. Influence of NaHCO3 soaking on phytic acid content of soybean sprouts and the optimization of its germination conditions%NaHCO3浸泡对发芽大豆植酸含量影响及其发芽条件优化

    Institute of Scientific and Technical Information of China (English)

    仲磊; 王新坤; 杨润强; 郭丽萍; 顾振新

    2014-01-01

    研究NaHCO3浸泡与发芽条件对大豆中植酸含量的影响并对其进行优化,探讨不同发芽时间下植酸含量、植酸酶活性、植酸与总锌分子质量比值(PA/Zn)、植酸与总铁分子质量比值(PA/Fe)的变化.以大豆籽粒为试材,去离子水预浸泡为对照,通过单因素试验(NaHCO3浸泡浓度、浸泡时间和发芽温度)与正交试验(L9(33))对发芽条件进行优化,并在最优发芽条件下,测定不同发芽时间时植酸含量、植酸酶活性、总锌和总铁含量.结果表明:用0.50 g·L-1NaHCO3浸泡处理的大豆,植酸含量在发芽0d时比对照降低11.94%;发芽4d后,与对照无显著差异.在发芽温度33℃、浸泡时间3h和NaHCO3质量浓度0.20 g·L-1的最优条件下,发芽4d的大豆中植酸含量降低至(7.92+-0.02) mg·g-1,与原料相比,降幅为45.75%;发芽开始时(0 d),0.20 g·L-1NaHCO3浸泡处理的大豆植酸酶活性高于对照,随着发芽的进行,2种浸泡处理间无显著差异;最优条件下发芽3~4d时,0.20 g·L-1NaHCO3浸泡处理的发芽大豆中植酸含量、PA/Zn和PA/Fe值均比对照低.表明:大豆籽粒发芽前使用NaHCO3浸泡可显著改善发芽大豆中Zn与Fe的生物利用率,其中Fe的生物利用率可达最优值.

  16. Microbial phytases in phosphorus acquisition and plant growth promotion

    OpenAIRE

    2011-01-01

    Phosphorus (P) is one of the major constituents in energy metabolism and biosynthesis of nucleic acids and cell membranes with an important role in regulation of a number of enzymes. Soil phosphorous is an important macronutrient for plant growth. Phosphorus deficiency in soil is a major problem for agricultural production. Total soil P occurs in either organic or in organic form. Phytic acid as phytate (salts of phytic acid) is the major form of organic phosphorus in soil and it is not readi...

  17. Demospongic Acids Revisited

    Directory of Open Access Journals (Sweden)

    Gilles Barnathan

    2010-10-01

    Full Text Available The well-known fatty acids with a D5,9 unsaturation system were designated for a long period as demospongic acids, taking into account that they originally occurred in marine Demospongia sponges. However, such acids have also been observed in various marine sources with a large range of chain-lengths (C16–C32 and from some terrestrial plants with short acyl chains (C18–C19. Finally, the D5,9 fatty acids appear to be a particular type of non-methylene-interrupted fatty acids (NMA FAs. This article reviews the occurrence of these particular fatty acids in marine and terrestrial organisms and shows the biosynthetic connections between D5,9 fatty acids and other NMI FAs.

  18. Acidic Ionic Liquids.

    Science.gov (United States)

    Amarasekara, Ananda S

    2016-05-25

    Ionic liquid with acidic properties is an important branch in the wide ionic liquid field and the aim of this article is to cover all aspects of these acidic ionic liquids, especially focusing on the developments in the last four years. The structural diversity and synthesis of acidic ionic liquids are discussed in the introduction sections of this review. In addition, an unambiguous classification system for various types of acidic ionic liquids is presented in the introduction. The physical properties including acidity, thermo-physical properties, ionic conductivity, spectroscopy, and computational studies on acidic ionic liquids are covered in the next sections. The final section provides a comprehensive review on applications of acidic ionic liquids in a wide array of fields including catalysis, CO2 fixation, ionogel, electrolyte, fuel-cell, membrane, biomass processing, biodiesel synthesis, desulfurization of gasoline/diesel, metal processing, and metal electrodeposition.

  19. [Biosynthesis of adipic acid].

    Science.gov (United States)

    Han, Li; Chen, Wujiu; Yuan, Fei; Zhang, Yuanyuan; Wang, Qinhong; Ma, Yanhe

    2013-10-01

    Adipic acid is a six-carbon dicarboxylic acid, mainly for the production of polymers such as nylon, chemical fiber and engineering plastics. Its annual demand is close to 3 million tons worldwide. Currently, the industrial production of adipic acid is based on the oxidation of aromatics from non-renewable petroleum resources by chemo-catalytic processes. It is heavily polluted and unsustainable, and the possible alternative method for adipic acid production should be developed. In the past years, with the development of synthetic biology and metabolic engineering, green and clean biotechnological methods for adipic acid production attracted more attention. In this study, the research advances of adipic acid and its precursor production are reviewed, followed by addressing the perspective of the possible new pathways for adipic acid production.

  20. Boric acid and boronic acids inhibition of pigeonpea urease.

    Science.gov (United States)

    Reddy, K Ravi Charan; Kayastha, Arvind M

    2006-08-01

    Urease from the seeds of pigeonpea was competitively inhibited by boric acid, butylboronic acid, phenylboronic acid, and 4-bromophenylboronic acid; 4-bromophenylboronic acid being the strongest inhibitor, followed by boric acid > butylboronic acid > phenylboronic acid, respectively. Urease inhibition by boric acid is maximal at acidic pH (5.0) and minimal at alkaline pH (10.0), i.e., the trigonal planar B(OH)3 form is a more effective inhibitor than the tetrahedral B(OH)4 -anionic form. Similarly, the anionic form of phenylboronic acid was least inhibiting in nature.

  1. Arsenic-hyperaccumulator Pteris vittata efficiently solubilized phosphate rock to sustain plant growth and As uptake.

    Science.gov (United States)

    Fu, Jing-Wei; Liu, Xue; Han, Yong-He; Mei, Hanyi; Cao, Yue; de Oliveira, Letuzia M; Liu, Yungen; Rathinasabapathi, Bala; Chen, Yanshan; Ma, Lena Q

    2017-01-31

    Phosphorus (P) is one of the most important nutrients for phytoremediation of arsenic (As)-contaminated soils. In this study, we demonstrated that As-hyperaccumulator Pteris vittata was efficient in acquiring P from insoluble phosphate rock (PR). When supplemented with PR as the sole P source in hydroponic systems, P. vittata accumulated 49% and 28% higher P in the roots and fronds than the -P treatment. In contrast, non-hyperaccumulator Pteris ensiformis was unable to solubilize P from PR. To gain insights into PR solubilization by plants, organic acids in plant root exudates were analyzed by HPLC. The results showed that phytic acid was the predominant (>90%) organic acid in P. vittata root exudates whereas only oxalic acid was detected in P. ensiformis. Moreover, P. vittata secreted more phytic acid in -P and PR treatments. Compared to oxalic acid, phytic acid was more effective in solubilizing PR, suggesting that phytic acid was critical for PR utilization. Besides, secretion of phytic acid by P. vittata was not inhibited by arsenate. Our data indicated that phytic acid played an important role in efficient use of insoluble PR by P. vittata, shedding light on using insoluble PR to enhance phytoremediation of As-contaminated soils.

  2. [Improved biological availability of zinc in coarse meal and crisp meal bread].

    Science.gov (United States)

    Harmuth-Hoene, A E; Meuser, F

    1988-12-01

    A study was undertaken to determine whether the reduction of the molar phytic acid/zinc ratio by enrichment of wholemeal and crispbread with zinc improves zinc availability to the same extent as does the reduction of phytic acid in whole meal cereal products. For three weeks, five diets (three samples of wholemeal bread and two samples of crispbread] were fed to growing rats. Significant increases in bone-zinc deposition and activity of serum alkaline phosphatase were induced by those bread samples in which the phytic acid/zinc ratio had been lowered either by enrichment with zinc or by reduction of phytic acid (enzymatic hydrolysis by adjusting the pH-value of the dough with lactic acid) when compared with untreated bread samples. Weight gain of the animals differed little between the groups. The results suggest that it is possible to improve zinc availability in wholemeal cereal products high in phytate by enrichment with zinc.

  3. Acid-Base Homeostasis.

    Science.gov (United States)

    Hamm, L Lee; Nakhoul, Nazih; Hering-Smith, Kathleen S

    2015-12-07

    Acid-base homeostasis and pH regulation are critical for both normal physiology and cell metabolism and function. The importance of this regulation is evidenced by a variety of physiologic derangements that occur when plasma pH is either high or low. The kidneys have the predominant role in regulating the systemic bicarbonate concentration and hence, the metabolic component of acid-base balance. This function of the kidneys has two components: reabsorption of virtually all of the filtered HCO3(-) and production of new bicarbonate to replace that consumed by normal or pathologic acids. This production or generation of new HCO3(-) is done by net acid excretion. Under normal conditions, approximately one-third to one-half of net acid excretion by the kidneys is in the form of titratable acid. The other one-half to two-thirds is the excretion of ammonium. The capacity to excrete ammonium under conditions of acid loads is quantitatively much greater than the capacity to increase titratable acid. Multiple, often redundant pathways and processes exist to regulate these renal functions. Derangements in acid-base homeostasis, however, are common in clinical medicine and can often be related to the systems involved in acid-base transport in the kidneys.

  4. Glycolic Acid 15% Plus Salicylic Acid 2%

    Science.gov (United States)

    Sánchez-Blanco, Elena

    2011-01-01

    Background: Facial flat warts are a contagious viral disease that can cause disturbing cosmetic problems. Topical glycolic acid has been reported to be effective in dermatological treatment depending on the exfoliant capacity, but has not often been reported to be effective in the treatment of facial flat warts. Objective: The aim of this paper was to evaluate the efficacy and safety of glycolic acid 15% topical gel plus salicylic acid 2% in the treatment of recalcitrant facial flat warts. Methods: A total of 20 consecutive patients 7 to 16 years of age with recalcitrant facial flat warts were enrolled in this study. Patients having warts by the eye and lip regions were excluded from the study. A fine layer of face gel was applied to the treatment area once daily. Most of the participants had tried different treatments with no success. Assessments for the response and the occurrence of side effects were performed every two weeks at Weeks 2, 4, 6, and 8. Results: All the patients were clinically cured within eight weeks. Seven patients cleared in four weeks, and 13 patients cleared in eight weeks. No noticeable adverse events were related to the skin. Conclusion: Topical gel of glycolic acid 15% plus salicylic acid 2% is safe and effective when applied to facial flat warts once daily until clearance and may be considered as first-line treatment. PMID:21938272

  5. Nitrogen Lewis Acids.

    Science.gov (United States)

    Pogoreltsev, Alla; Tulchinsky, Yuri; Fridman, Natalia; Gandelman, Mark

    2017-03-22

    Being a major conception of chemistry, Lewis acids have found countless applications throughout chemical enterprise. Although many chemical elements can serve as the central atom of Lewis acids, nitrogen is usually associated with Lewis bases. Here, we report on the first example of robust and modifiable Lewis acids centered on the nitrogen atom, which provide stable and well-characterized adducts with various Lewis bases. On the basis of the reactivity of nitrogen Lewis acids, we prepared, for the first time, cyclic triazanes, a class of cyclic organic compounds sequentially bearing three all-saturated nitrogen atoms (N-N-N motif). Reactivity abilities of these N-Lewis acids were explained by theoretical calculations. Properties and future applications of nitrogen Lewis acids are intriguing.

  6. Citric Acid Alternative to Nitric Acid Passivation

    Science.gov (United States)

    Lewis, Pattie L. (Compiler)

    2013-01-01

    The Ground Systems Development and Operations GSDO) Program at NASA John F. Kennedy Space Center (KSC) has the primary objective of modernizing and transforming the launch and range complex at KSC to benefit current and future NASA programs along with other emerging users. Described as the launch support and infrastructure modernization program in the NASA Authorization Act of 2010, the GSDO Program will develop and implement shared infrastructure and process improvements to provide more flexible, affordable, and responsive capabilities to a multi-user community. In support of the GSDO Program, the purpose of this project is to demonstratevalidate citric acid as a passivation agent for stainless steel. Successful completion of this project will result in citric acid being qualified for use as an environmentally preferable alternative to nitric acid for passivation of stainless steel alloys in NASA and DoD applications.

  7. USGS Tracks Acid Rain

    Science.gov (United States)

    Gordon, John D.; Nilles, Mark A.; Schroder, LeRoy J.

    1995-01-01

    The U.S. Geological Survey (USGS) has been actively studying acid rain for the past 15 years. When scientists learned that acid rain could harm fish, fear of damage to our natural environment from acid rain concerned the American public. Research by USGS scientists and other groups began to show that the processes resulting in acid rain are very complex. Scientists were puzzled by the fact that in some cases it was difficult to demonstrate that the pollution from automobiles and factories was causing streams or lakes to become more acidic. Further experiments showed how the natural ability of many soils to neutralize acids would reduce the effects of acid rain in some locations--at least as long as the neutralizing ability lasted (Young, 1991). The USGS has played a key role in establishing and maintaining the only nationwide network of acid rain monitoring stations. This program is called the National Atmospheric Deposition Program/National Trends Network (NADP/NTN). Each week, at approximately 220 NADP/NTN sites across the country, rain and snow samples are collected for analysis. NADP/NTN site in Montana. The USGS supports about 72 of these sites. The information gained from monitoring the chemistry of our nation's rain and snow is important for testing the results of pollution control laws on acid rain.

  8. Parenteral Nutrition: Amino Acids

    Science.gov (United States)

    Hoffer, Leonard John

    2017-01-01

    There is growing interest in nutrition therapies that deliver a generous amount of protein, but not a toxic amount of energy, to protein-catabolic critically ill patients. Parenteral amino acids can achieve this goal. This article summarizes the biochemical and nutritional principles that guide parenteral amino acid therapy, explains how parenteral amino acid solutions are formulated, and compares the advantages and disadvantages of different parenteral amino acid products with enterally-delivered whole protein products in the context of protein-catabolic critical illness. PMID:28287411

  9. Parenteral Nutrition: Amino Acids.

    Science.gov (United States)

    Hoffer, Leonard John

    2017-03-10

    There is growing interest in nutrition therapies that deliver a generous amount of protein, but not a toxic amount of energy, to protein-catabolic critically ill patients. Parenteral amino acids can achieve this goal. This article summarizes the biochemical and nutritional principles that guide parenteral amino acid therapy, explains how parenteral amino acid solutions are formulated, and compares the advantages and disadvantages of different parenteral amino acid products with enterally-delivered whole protein products in the context of protein-catabolic critical illness.

  10. Improvement on Acid-solubility of Soy Protein Isolate with Enzymatic Method%酶法提高大豆分离蛋白酸溶性的研究

    Institute of Scientific and Technical Information of China (English)

    黄橙子; 王静; 高红亮; 崔红亮; 常忠义

    2013-01-01

    通过单因素试验研究了植酸酶添加量、酶解时间、pH、温度和料液浓度对大豆蛋白酸溶度、透光率和料液粘度的影响,在单因素试验的基础上对pH、温度、料液浓度和时间进行四因素三水平的正交试验,结果表明,当植酸酶添加量为0.6%时,酶解的最佳条件组合为pH3.0,温度40℃,料液浓度10%,酶解时间45 min,此时大豆分离蛋白的酸溶度为45.79%,透光率为68.5%,与优化前相比,分别提高了30.65%和29.4%.%Soy protein isolate(SPI)has been used in the food industry for decades,but its application in acidic food products was Limited because of its low solubility in the acidic condition. The objective of the experiments was to improve the solubility of SPI under acidic conditions by hydrolyzing the phytic acid with phytase and to find the optimum conditions for the enzymatic hydrolyzing process. During the experiments, acid-soluble properties,including acid-solubility,transmittance,and viscosity of the processed material have been observed. Single factor and orthogonal experiments revealed that when the solid content of the curd slurry was 10% ,the best phytase adding amount was 0. 6% ,the ideal condition for the enzymatic reaction was pH3.0, 40℃,45 min. After the enzymatic treatment,acid-solubility and transmittance of SPI reached 45.79% and 68. 5% ,increased by 30.65% and 29.4% ,respectively.

  11. Diterpenoid acids from Grindelia nana.

    Science.gov (United States)

    Mahmoud, A A; Ahmed, A A; Tanaka, T; Iinuma, M

    2000-03-01

    Two new norditerpenoid acids of the labdane-type (norgrindelic acids), 4,5-dehydro-6-oxo-18-norgrindelic acid (1) and 4beta-hydroxy-6-oxo-19-norgrindelic acid (2), as well as a new grindelic acid derivative, 18-hydroxy-6-oxogrindelic acid (3), were isolated from the aerial parts of Grindelia nana. In addition, the known compounds, 6-oxogrindelic acid, grindelic acid, methyl grindeloate, 7alpha,8alpha-epoxygrindelic acid, and 4alpha-carboxygrindelic acid were also isolated. The structures of the new compounds were characterized on the basis of spectroscopic analysis.

  12. Download this PDF file

    African Journals Online (AJOL)

    compaq

    2012-12-07

    Dec 7, 2012 ... boiling, drying and dehulling resulted in highest reduction in antinutritional factors with minimal nutrient ..... (mg/100 g). Manganese. (mg/100 g) .... Inhibitor, Phytic Acid and Tannins of Soybean (Glycine max Merr.), Cowpea.

  13. Unraveling the major triticeae phytases – towards cereals with high grain phytase activity

    DEFF Research Database (Denmark)

    Madsen, Claus Krogh

    Phytic acid is the main storage form of phosphate in plant seeds. Phosphorus in this form is largely indigestible by monogastric animals due to insufficient activity of the hydrolytic enzyme phytase in their digestive tract and in most plant seeds. Moreover as a strong chelator, undigested phytic...... acid constitutes the most important anti nutritional factor for the bioavailability of minerals. However, the mature grain phytase activity varies considerably between plant species and between cultivars. Cereals like rye, wheat and barley possess significant phytase activity with the potential...... to contribute to the hydrolysis of phytic acid in food and feed. In contrast rice and maize possess insufficient phytase activity in the mature grain to have a notable effect on the phytic acid. The aim of the present study was to (1) uncover the genetic basis for the major phytases in cereal grains (2) explain...

  14. Zinc absorption in humans from meals based on rye, barley, oatmeal, triticale and whole wheat

    Energy Technology Data Exchange (ETDEWEB)

    Sandstroem, B.A.; Almgren, A.; Kivistoe, B.C.; Cederblad, A.

    1987-11-01

    The absorption of zinc from meals based on 60 g of rye, barley, oatmeal, triticale or whole wheat was studied by use of extrinsic labelling with /sup 65/Zn and measurement of the whole-body retention of the radionuclide. The cereals were prepared in the form of bread or porridge and were served with 200 mL of milk. The oatmeal flakes were also served without further preparation. The absorption of zinc was negatively correlated to the phytic acid content of the meal with the highest absorption, 26.8 +/- 7.4%, from the rye bread meal containing 100 mumol of phytic acid and the lowest, 8.4 +/- 1.0%, from oatmeal porridge with a phytic acid content of 600 mumol. It is concluded that food preparation that decreases the phytic acid content improves zinc absorption.

  15. Variability of antinutritive compounds in flaxseed flours

    Directory of Open Access Journals (Sweden)

    Roberto Russo

    2013-09-01

    Full Text Available The use of flaxseed flours in the diets of poultry and broilers may be limited by the presence of antinutritive compounds. The content of cyanogenic glycosides, phytic acid, condensed tannins and trypsin inhibitors was evaluated in seven varieties of Linum usitatissimum. Phytic acid, condensed tannins and trypsin inhibitors showed significant differences among varieties. Only the concentration of cyanogenic glycosides and phytic acid in the flour deserves attention, while the content of condensed tannins and trypsin inhibitors are to acceptable levels. Since the flax meal is an important source of omega-3 for poultry and broilers, the cyanogenic glycoside and phytic acid contents in linseed has to be reduced to increase the ration to be included in the diet.

  16. Zinc absorption in humans from meals based on rye, barley, oatmeal, triticale and whole wheat.

    Science.gov (United States)

    Sandström, B; Almgren, A; Kivistö, B; Cederblad, A

    1987-11-01

    The absorption of zinc from meals based on 60 g of rye, barley, oatmeal, triticale or whole wheat was studied by use of extrinsic labelling with 65Zn and measurement of the whole-body retention of the radionuclide. The cereals were prepared in the form of bread or porridge and were served with 200 mL of milk. The oatmeal flakes were also served without further preparation. The absorption of zinc was negatively correlated to the phytic acid content of the meal with the highest absorption, 26.8 +/- 7.4%, from the rye bread meal containing 100 mumol of phytic acid and the lowest, 8.4 +/- 1.0%, from oatmeal porridge with a phytic acid content of 600 mumol. It is concluded that food preparation that decreases the phytic acid content improves zinc absorption.

  17. Nucleic Acid Immunity.

    Science.gov (United States)

    Hartmann, G

    2017-01-01

    Organisms throughout biology need to maintain the integrity of their genome. From bacteria to vertebrates, life has established sophisticated mechanisms to detect and eliminate foreign genetic material or to restrict its function and replication. Tremendous progress has been made in the understanding of these mechanisms which keep foreign or unwanted nucleic acids from viruses or phages in check. Mechanisms reach from restriction-modification systems and CRISPR/Cas in bacteria and archaea to RNA interference and immune sensing of nucleic acids, altogether integral parts of a system which is now appreciated as nucleic acid immunity. With inherited receptors and acquired sequence information, nucleic acid immunity comprises innate and adaptive components. Effector functions include diverse nuclease systems, intrinsic activities to directly restrict the function of foreign nucleic acids (e.g., PKR, ADAR1, IFIT1), and extrinsic pathways to alert the immune system and to elicit cytotoxic immune responses. These effects act in concert to restrict viral replication and to eliminate virus-infected cells. The principles of nucleic acid immunity are highly relevant for human disease. Besides its essential contribution to antiviral defense and restriction of endogenous retroelements, dysregulation of nucleic acid immunity can also lead to erroneous detection and response to self nucleic acids then causing sterile inflammation and autoimmunity. Even mechanisms of nucleic acid immunity which are not established in vertebrates are relevant for human disease when they are present in pathogens such as bacteria, parasites, or helminths or in pathogen-transmitting organisms such as insects. This review aims to provide an overview of the diverse mechanisms of nucleic acid immunity which mostly have been looked at separately in the past and to integrate them under the framework nucleic acid immunity as a basic principle of life, the understanding of which has great potential to

  18. Peptide Nucleic Acids (PNA)

    DEFF Research Database (Denmark)

    2002-01-01

    A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker....

  19. Peptide Nucleic Acids

    DEFF Research Database (Denmark)

    1998-01-01

    A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker....

  20. Peptide Nucleic Acids

    DEFF Research Database (Denmark)

    2003-01-01

    A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker....

  1. Peptide Nucleic Acids

    DEFF Research Database (Denmark)

    2004-01-01

    A novel class of compounds known as peptide nucleic acids, bind complementary DNA and RNA strands, and generally do so more strongly than the corresponding DNA or RNA strands while exhibiting increased sequence specificity and solubility. The peptide nucleic acids comprise ligands selected from...

  2. Carbolic acid poisoning

    Science.gov (United States)

    ... you to. If the person swallowed the carbolic acid, give them water or milk right away, if a provider tells ... well someone does depends on how much carbolic acid they swallowed and how quickly they receive treatment. The faster medical help is given, the better ...

  3. Uric acid - blood

    Science.gov (United States)

    ... High levels of uric acid can sometimes cause gout or kidney disease. You may have this test if you have had or are about to have certain types of chemotherapy. Rapid weight loss, which may occur with such treatments, can increase the amount of uric acid in ...

  4. Neurotoxicity of Folic Acid

    NARCIS (Netherlands)

    Amsterdam van JGC; Jansen EHJM; A Opperhuizen; TOX

    2004-01-01

    The present review summarises the neurotoxicological effects of folic acid. Some studies in animals have shown that folic acid is neurotoxic and epileptogenic when applied directly to the brain. One poorly controlled and not further reproduced study from 1970 reported neurotoxic symptoms like

  5. Amino Acid Crossword Puzzle

    Science.gov (United States)

    Sims, Paul A.

    2011-01-01

    Learning the 20 standard amino acids is an essential component of an introductory course in biochemistry. Later in the course, the students study metabolism and learn about various catabolic and anabolic pathways involving amino acids. Learning new material or concepts often is easier if one can connect the new material to what one already knows;…

  6. Fats and fatty acids

    Science.gov (United States)

    The absolute fat requirement of the human species is the amount of essential fatty acids needed to maintain optimal fatty acid composition of all tissues and normal eicosanoid synthesis. At most, this requirement is no more than about 5% of an adequate energy intake. However, fat accounts for appro...

  7. Peptide Nucleic Acid Synthons

    DEFF Research Database (Denmark)

    2004-01-01

    A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker....

  8. Chlorogenic acid and caffeic acid are absorbed in humans

    OpenAIRE

    2001-01-01

    Chlorogenic acid, an ester of caffeic acid and quinic acid, is a major phenolic compound in coffee; daily intake in coffee drinkers is 0.5-1 g. Chlorogenic acid and caffeic acid are antioxidants in vitro and might therefore contribute to the prevention of cardiovascular disease. However, data on the absorption of chlorogenic acid and caffeic acid in humans are lacking. We determined the absorption of chlorogenic acid and caffeic acid in a cross-over study with 4 female and 3 male healthy ileo...

  9. Chlorogenic acid and caffeic acid are absorbed in humans

    NARCIS (Netherlands)

    Olthof, M.R.; Hollman, P.C.H.; Katan, M.B.

    2001-01-01

    Chlorogenic acid, an ester of caffeic acid and quinic acid, is a major phenolic compound in coffee; daily intake in coffee drinkers is 0.5-1 g. Chlorogenic acid and caffeic acid are antioxidants in vitro and might therefore contribute to the prevention of cardiovascular disease. However, data on the

  10. Chlorogenic acid and caffeic acid are absorbed in humans

    NARCIS (Netherlands)

    Olthof, M.R.; Hollman, P.C.H.; Katan, M.B.

    2001-01-01

    Chlorogenic acid, an ester of caffeic acid and quinic acid, is a major phenolic compound in coffee; daily intake in coffee drinkers is 0.5-1 g. Chlorogenic acid and caffeic acid are antioxidants in vitro and might therefore contribute to the prevention of cardiovascular disease. However, data on the

  11. Halogenated fatty acids

    DEFF Research Database (Denmark)

    Mu, Huiling; Wesén, Clas; Sundin, Peter

    1997-01-01

    Chlorinated fatty acids have been found to be major contributors to organohalogen compounds in fish, bivalves, jellyfish, and lobster, and they have been indicated to contribute considerably to organohalogens in marine mammals. Brominated fatty acids have been found in marine sponges. Also......, chlorinated lipids have been found in meat exposed to hypochlorite disinfected water, and in chlorine-treated flour and in products made from such flour. Following exposure to chlorine bleached pulp mill effluents, aquatic organisms may have elevated concentrations of chlorinated fatty acids in their lipids....... However, a natural production of halogenated fatty acids is also possible. In this paper we summarize the present knowledge of the occurrence of halogenated fatty acids in lipids and suggested ways of their formation. In Part II (Trends Anal. Chem. 16 (1997) 274) we deal with methods...

  12. Phenolic acids enzymatic lipophilization.

    Science.gov (United States)

    Figueroa-Espinoza, Maria-Cruz; Villeneuve, Pierre

    2005-04-20

    Lipophilization is the esterification of a lipophilic moiety (fatty acid or fatty alcohol) on different substrates (phenolic acid, sugar, protein, ...), resulting in new molecules with modified hydrophilic/lipophilic balance. This reaction can be obtained chemically or enzymatically using different enzymes. Phenolic acids possess interesting biological properties (antioxidant, chelator, free radical scavenger, UV filter, antimicrobial, ...), but because of their relatively low solubility in aprotic media, their application in oil-based products is limited. Therefore, the esterification of their carboxylic acid function with a fatty alcohol enhances their hydrophobicity and results in a multifunctional amphiphilic molecule. Enzymatic lipophilization of phenolic acids is nowadays studied for potential industrial applications. Different systems have been proposed to perform the reaction yield [free or immobilized enzymes (lipase, feruloyl esterase, tannase, etc.), free or added organic solvent, addition of surfactant, microemulsion system, etc.]. Some of the functional properties of these esters have been demonstrated. This review presents a panorama of the advances in this field.

  13. 2-Methylaspartic acid monohydrate

    Directory of Open Access Journals (Sweden)

    Ray J. Butcher

    2013-12-01

    Full Text Available The title compound, C5H9NO4·H2O, is an isomer of the α-amino acid glutamic acid that crystallizes from water in its zwitterionic form as a monohydrate. It is not one of the 20 proteinogenic α-amino acids that are used in living systems and differs from the natural amino acids in that it has an α-methyl group rather than an α-H atom. In the crystal, an O—H...O hydrogen bond is present between the acid and water molecules while extensive N—H...O and O—H...O hydrogen bonds link the components into a three-dimensional array.

  14. SCREENING OF THERMOPHYLIC MICROORGANISM FROM IJEN CRATER BANYUWANGI AS PHYTASE ENZYME PRODUCER

    OpenAIRE

    Kusumadjaja, Aline Puspita; Budiati, Tutuk; Puspaningsih, Ni Nyoman Tri; Sajidan, Sajidan

    2010-01-01

    Phytase is enzyme which hydrolysis phytic acid to anorganic phosphate and myo-inositol pentakis-, tetrakis-, tris-, bis-, and monophosphate. The use of phytase in feed industry can overcome environment and nutrition problems which were arisen from unmetabolism phytic acid or its salt by poultry, swine and fish. The feed industry needs a thermostable enzyme due to the need of high temperature in pelleting process, i.e. 81 °C. By using thermostabile phytase, the pelleting process will not affec...

  15. Composition of amino acids, fatty acids and dietary fibre monomers ...

    African Journals Online (AJOL)

    Composition of amino acids, fatty acids and dietary fibre monomers in kernels of ... Nuts are rich in protein and essential amino acids, and have a high energy value ... of protein, especially when combined with foods with high lysine content.

  16. Amino acids analysis during lactic acid fermentation by single strain ...

    African Journals Online (AJOL)

    SAM

    2014-07-09

    Jul 9, 2014 ... of the three LAB strains to utilize amino acids for growth and lactic acid production were employed to ... Lactic acid bacteria (LAB), which are used for the ..... and characterization of potential probiotic lactobacilli from pig feces.

  17. Trans Fatty Acids

    Science.gov (United States)

    Doyle, Ellin

    1997-09-01

    Fats and their various fatty acid components seem to be a perennial concern of nutritionists and persons concerned with healthful diets. Advice on the consumption of saturated, polyunsaturated, monounsaturated, and total fat bombards us from magazines and newspapers. One of the newer players in this field is the group of trans fatty acids found predominantly in partially hydrogenated fats such as margarines and cooking fats. The controversy concerning dietary trans fatty acids was recently addressed in an American Heart Association (AHA) science advisory (1) and in a position paper from the American Society of Clinical Nutrition/American Institute of Nutrition (ASCN/AIN) (2). Both reports emphasize that the best preventive strategy for reducing risk for cardiovascular disease and some types of cancer is a reduction in total and saturated fats in the diet, but a reduction in the intake of trans fatty acids was also recommended. Although the actual health effects of trans fatty acids remain uncertain, experimental evidence indicates that consumption of trans fatty acids adversely affects serum lipid levels. Since elevated levels of serum cholesterol and triacylglycerols are associated with increased risk of cardiovascular disease, it follows that intake of trans fatty acids should be minimized.

  18. Gluconic acid production.

    Science.gov (United States)

    Anastassiadis, Savas; Morgunov, Igor G

    2007-01-01

    Gluconic acid, the oxidation product of glucose, is a mild neither caustic nor corrosive, non toxic and readily biodegradable organic acid of great interest for many applications. As a multifunctional carbonic acid belonging to the bulk chemicals and due to its physiological and chemical characteristics, gluconic acid itself, its salts (e.g. alkali metal salts, in especially sodium gluconate) and the gluconolactone form have found extensively versatile uses in the chemical, pharmaceutical, food, construction and other industries. Present review article presents the comprehensive information of patent bibliography for the production of gluconic acid and compares the advantages and disadvantages of known processes. Numerous manufacturing processes are described in the international bibliography and patent literature of the last 100 years for the production of gluconic acid from glucose, including chemical and electrochemical catalysis, enzymatic biocatalysis by free or immobilized enzymes in specialized enzyme bioreactors as well as discontinuous and continuous fermentation processes using free growing or immobilized cells of various microorganisms, including bacteria, yeast-like fungi and fungi. Alternatively, new superior fermentation processes have been developed and extensively described for the continuous and discontinuous production of gluconic acid by isolated strains of yeast-like mold Aureobasidium pullulans, offering numerous advantages over the traditional discontinuous fungi processes.

  19. Sulfuric Acid on Europa

    Science.gov (United States)

    1999-01-01

    Frozen sulfuric acid on Jupiter's moon Europa is depicted in this image produced from data gathered by NASA's Galileo spacecraft. The brightest areas, where the yellow is most intense, represent regions of high frozen sulfuric acid concentration. Sulfuric acid is found in battery acid and in Earth's acid rain. This image is based on data gathered by Galileo's near infrared mapping spectrometer.Europa's leading hemisphere is toward the bottom right, and there are enhanced concentrations of sulfuric acid in the trailing side of Europa (the upper left side of the image). This is the face of Europa that is struck by sulfur ions coming from Jupiter's innermost moon, Io. The long, narrow features that crisscross Europa also show sulfuric acid that may be from sulfurous material extruded in cracks. Galileo, launched in 1989, has been orbiting Jupiter and its moons since December 1995. JPL manages the Galileo mission for NASA's Office of Space Science, Washington DC. JPL is a division of the California Institute of Technology, Pasadena, CA.

  20. Fusidic acid in dermatology

    DEFF Research Database (Denmark)

    Schöfer, Helmut; Simonsen, Lene

    1995-01-01

    efficacy and tolerability. Similarly, plain fusidic acid cream or ointment used two or three times daily in SSTIs such as impetigo are clinically and bacteriologically effective, with minimal adverse events. Combination formulations of fusidic acid with 1% hydrocortisone or 0.1% betamethasone achieve...... excellent results in infected eczema by addressing both inflammation and infection. A new lipid-rich combination formulation provides an extra moisturizing effect. Development of resistance to fusidic acid has remained generally low or short-lived and can be minimized by restricting therapy to no more than...