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Sample records for phragmites australis plantlets

  1. Geographic variation in growth responses in Phragmites australis

    NARCIS (Netherlands)

    Clevering, O.A.; Brix, H.; Lukavska, J.

    2001-01-01

    Phragmites australis is a cosmopolitan wetlands species occurring in a wide range of climatic habitats, It can be assumed that adaptations to climate have evolved to enable the synchronization of growth with the seasonality of the environment. To study these adaptations, European P. australis was

  2. Can nutrient enrichment influence the invasion of Phragmites australis?

    Science.gov (United States)

    Uddin, Md Nazim; Robinson, Randall William

    2018-02-01

    Plant invasion and nutrient enrichment because of anthropogenic landscape modifications seriously threaten native plant community diversity in aquatic and wetland ecosystems. It is poorly understood, however, whether these two disturbances interact with the functional identity of recipient native plants to drive community change. We performed combined studies in the fields and greenhouse to examine whether nutrient enrichment may trigger the invasion of Phragmites australis in wetlands through competitive advantage over native Melaleuca ericifolia. Chemical characterizations of rhizosphere water were distinguished in two different nutrient enriched wetlands associated with and without Phragmites over the seasons. Significant changes in rhizosphere water were observed in invaded area compared to uninvaded area at both sites. High nitrogen (NO 3 - ), phosphorous (PO 4 3- ), dissolved organic carbon, phenolics contents, with low pH were found in invaded areas compared to uninvaded areas. Total biomass of Phragmites was positively regressed with rhizosphere water nitrogen (NO 3 - ) and phosphorous (PO 4 3- ) content. Nutrient addition significantly enhanced the growth and competitive ability of Phragmites over Melaleuca. In contrast, Melaleuca was significantly less competitive than Phragmites. There was a significantly positive correlation between the growth of Phragmites grown alone and its competitive ability. The findings in greenhouse studies coupled with characteristics of Phragmites and its' rhizosphere chemistry in the nutrient enriched fields suggest that nutrient enrichment may enhance Phragmites invasion through correspondingly increasing growth and maintaining inherent competitive advantages of Phragmites. Nutrient management could limit the vigorous growth of Phragmites in wetlands and thereby reduce invasion through competitive advantages over natives, which might have important management implications for wetland managers. Copyright © 2017. Published by

  3. JST Thesaurus Headwords and Synonyms: Phragmites australis [MeCab user dictionary for science technology term[Archive

    Lifescience Database Archive (English)

    Full Text Available MeCab user dictionary for science technology term Phragmites australis 名詞 一般 * * * ...* ヨシ ヨシ ヨシ Thesaurus2015 200906077254295905 C LS06 UNKNOWN_2 Phragmites australis

  4. Mapping the change of Phragmites australis live biomass in the lower Mississippi River Delta marshes

    Science.gov (United States)

    Ramsey, Elijah W.; Rangoonwala, Amina

    2017-07-28

    Multiyear remote sensing mapping of the normalized difference vegetation index (NDVI) was carried out as an indicator of live biomass composition of the Phragmites australis (hereafter Phragmites) marsh in the lower Mississippi River Delta (hereafter delta) from 2014 to 2017. Maps of NDVI change showed that the Phragmites condition was fairly stable between May 2014 and July 2015. From July 2015 to April 2016 NDVI change indicated Phragmites suffered a widespread decline in the live biomass proportion.  Between April and September 2016, most marsh remained unchanged from the earlier period or showed improvement; although there were pockets of continued decline scattered throughout the lower delta. From September 2016 to May 2017 a pronounced and widely exhibited decline in the condition of Phragmites marsh again occurred throughout the lower delta. This final NDVI change mapping supported field observations of Phragmites decline during the same period.

  5. Accumulation of Metals and Boron in Phragmites australis Planted in Constructed Wetlands Polishing Real Electroplating Wastewater.

    Science.gov (United States)

    Sochacki, Adam; Guy, Bernard; Faure, Olivier; Surmacz-Górska, Joanna

    2015-01-01

    The concentration of metals (Al, Cu, Fe, Mn, Ni, Zn) and B were determined in the above- and belowground biomass of Phragmites australis collected from the microcosm constructed wetland system used for the polishing of real electroplating wastewater. Translocation factor and bioconcentration factor were determined. Pearson correlation test was used to determine correlation between metal concentration in substrate and above- and belowground parts of Phragmites australis. The obtained results suggested that Phragmites australis did not play a major role as an accumulator of metals. It was observed also that the substrate could have exerted an effect on the translocation of Ni, Cu, Zn and Mn. The analysed concentrations of metals and B in biomass were in the range or even below the concentrations reported in the literature with the exception of Ni. The aboveground biomass was found suitable as a composting input in terms of metals concentrations.

  6. Observations of the ipogean part of Typha Latifolia and Phragmites Australis; Osservazioni sull'apparato ipogeo di Typha Latifolia e Phragmites Australis

    Energy Technology Data Exchange (ETDEWEB)

    Borin, M.; Bonaiti, G. [Padua Univ., Padua (Italy). Dipt. di Agronomia Ambientale e Produzioni Vegetali

    2000-06-01

    Since the interest on the use of wetlands to provide treatment of wastewaters is increasing in Italy, and the role of plants for the high performance of such systems is essential, observations on the ipogean part of Typha latifolia and Phragmites australis have been conducted after one year of growth in cubic containers (0,8 cm each side) filled with loamy textured soil. The length of rhizomes, the rhizomes and roots biomass production, and the TKN immobilisation in rhizomes and roots have been measured in soil layers of 10 cm wide. Typha latifolia and Phragmites australis produced high quantities of biomass (33 and 39 t {center_dot} ha{sup 1} of dry weight respectively) and showed a high immobilisation of nitrogen (270 and 250 kg {center_dot} ha{sup 1} respectively). The hypogeal part of Typha latifolia was developed especially in the first layers, whereas the one of Phragmites australis was more distributed along the soil profile (94 and 69% of the total biomass in the first 30 cm respectively). [Italian] Considerato l'interesse crescente che si sta osservando in Italia per la fitodepurazione delle acque inquinate e vista l'importanza che le piante esercitano in questa tecnologia, si sono condotte delle osservazioni sullo sviluppo dell'apparato ipogeo di Typha latifolia L. e Phragmites australis dopo un anno di accrescimento in cassoni cubici (0,8 m di lato) riempiti con terreno a grana media. Sono stati rilevati, per strati di 10 cm di profondita', la lunghezza dei rizomi, la biomassa di rizomi e radici ed il contenuto di TKN in rizomi e radici. Typha latifolia e Phragmites australis hanno prodotto molta biomassa (33 e 39 t {center_dot} ha{sup 1}di sostanza secca rispettivamente) ed hanno immagazinato una notevole quantita' di azoto (270 e 250 kg {center_dot} ha{sup 1} rispettivamente). Lungo il profilo del terreno, l'apparato ipogeo di Typha latifolia e' risultato piu' concentrato nei primi strati di quanto osservato in

  7. Decomposition processes in soil of a healthy and a declining Phragmites australis stand

    Czech Academy of Sciences Publication Activity Database

    Šantrůčková, Hana; Picek, Tomáš; Šimek, Miloslav; Bauer, Václav; Kopecký, Jiří; Pechar, Libor; Lukavská, Jaroslava; Čížková, Hana

    2001-01-01

    Roč. 69, - (2001), s. 217-234 ISSN 0304-3770 R&D Projects: GA ČR GA206/96/0589 Institutional research plan: CEZ:AV0Z6066911 Keywords : decomposition * eutrophication * Phragmites australis Subject RIV: EH - Ecology, Behaviour Impact factor: 1.361, year: 2001

  8. Phylogeographic analyses and genetic structure illustrate the complex evolutionary history of Phragmites australis in Mexico.

    Science.gov (United States)

    Colin, Ricardo; Eguiarte, Luis E

    2016-05-01

    Genetic data suggest that three lineages of Phragmites australis are found in North America: the Native North American lineage, the Gulf Coast lineage, and the Invasive lineage. In Mexico, P. australis is a common species, but nothing is known about the distribution or ecology of these lineages. We examined the phylogeography of P. australis to analyze the current geographic distribution of genetic variation, demographic history, and dispersal patterns to better understand its evolutionary history in Mexico. We sampled 427 individuals from 28 populations. We used two noncoding regions of chloroplast DNA to estimate the levels of genetic variation and identified the genetic groups across the species' geographical range in Mexico. We compared the genealogical relationships among haplotypes with those previously reported. A hypothesis of demographic expansion was also tested for the Mexican P. australis lineages. We found 13 new haplotypes native to Mexico that might be undergoing an active process of expansion and diversification. Genealogical analyses provided evidence that two independent lineages of P. australis are present in Mexico. The invasive lineage was not detected with our sampling. Our estimates of population expansions in Mexico ranged from 0.202 to 0.726 mya. Phragmites australis is a native species that has been in Mexico for thousands of years. Genetic data suggest that climatic changes during the Pleistocene played an important role in the demographic expansion of the populations that constitute the different genetic groups of P. australis in Mexico. © 2016 Botanical Society of America.

  9. Functional role of bacteria from invasive Phragmites australis in promotion of host growth

    Science.gov (United States)

    Soares, M. A.; Li, H-Y; Kowalski, Kurt P.; Bergen, M.; Torres, M. S.; White, J. F.

    2016-01-01

    We hypothesize that bacterial endophytes may enhance the competitiveness and invasiveness of Phragmites australis. To evaluate this hypothesis, endophytic bacteria were isolated from P. australis. The majority of the shoot meristem isolates represent species from phyla Firmicutes, Proteobacteria, and Actinobacteria. We chose one species from each phylum to characterize further and to conduct growth promotion experiments in Phragmites. Bacteria tested include Bacillus amyloliquefaciens A9a, Achromobacter spanius B1, and Microbacterium oxydans B2. Isolates were characterized for known growth promotional traits, including indole acetic acid (IAA) production, secretion of hydrolytic enzymes, phosphate solubilization, and antibiosis activity. Potentially defensive antimicrobial lipopeptides were assayed for through application of co-culturing experiments and mass spectrometer analysis. B. amyloliquefaciens A9a and M. oxydans B2 produced IAA. B. amyloliquefaciens A9a secreted antifungal lipopeptides. Capability to promote growth of P. australis under low nitrogen conditions was evaluated in greenhouse experiments. All three isolates were found to increase the growth of P. australis under low soil nitrogen conditions and showed increased absorption of isotopic nitrogen into plants. This suggests that the Phragmites microbes we evaluated most likely promote growth of Phragmites by enhanced scavenging of nitrogenous compounds from the rhizosphere and transfer to host roots. Collectively, our results support the hypothesis that endophytic bacteria play a role in enhancing growth of P. australis in natural populations. Gaining a better understanding of the precise contributions and mechanisms of endophytes in enabling P. australis to develop high densities rapidly could lead to new symbiosis-based strategies for management and control of the host.

  10. Genetics, novel weapons and rhizospheric microcosmal signaling in the invasion of Phragmites australis.

    Science.gov (United States)

    Rudrappa, Thimmaraju; Bais, Harsh P

    2008-01-01

    Chemical communication and perception strategies between plants are highly sophisticated but are only partly understood. Among the different interactions, the suppressive interaction of a class of chemicals released by one plant through root exudates against the neighbouring plants (allelopathy) have been implicated in the invasiveness of many exotic weedy species. Phragmites australis (common reed) is one of the dominant colonizers of the North American wetland marshes and exhibits invasive behavior by virtually replacing the entire native vegetation in its niche. Recently, by adopting a systematic bioassay driven approach we elucidated the role of root derived allelopathy as one of the important mechanisms by which P. australis exerts its invasive behavior. Additionally, our recent preliminary data indicates the involvement of rhizobacterial signaling in the invasive success of P. australis. A better understanding of biochemical weaponry used by P. australis will aid scientists and technologists in addressing the impact of root secretions in invasiveness of weedy species and thus promote a more informed environmental stewardship.

  11. Heavy metal contents and transfer capacities of Phragmites australis and Suaeda salsa in the Yellow River Delta, China

    Science.gov (United States)

    Zhang, Shuai; Bai, Junhong; Wang, Wei; Huang, Laibing; Zhang, Guangliang; Wang, Dawei

    2018-04-01

    Plant samples including roots, stems and leaves of Phragmites australis and Suaeda salsa were collected in the short-term flooding and tidal flooding wetlands of the Yellow River Delta of China. Six heavy metals (e.g., As, Cd, Cr, Cu, Pb, and Zn) were measured in roots, stems and leaves of each plant species using inductively coupled plasma atomic absorption spectrometry (ICP-AAS) to investigate the levels, and transfer capabilities of heavy metals in these two plant species. Our results showed that in the tidal flooding wetlands, the contents of As, Cr and Cd in roots of Phragmites australis and Suaeda salsa were higher than those in their stems and leaves. Suaeda salsa showed higher contents of Pb and Zn in leaves than those in roots and stems, whereas lower levels of Pb and Zn were observed in Phragmites australis. In the short-term flooding wetlands, heavy metal contents exhibited a big difference between different tissues of Phragmites australis and Suaeda salsa, and both plant species showed higher levels of Pb and Zn in leaves. Suaeda salsa roots enriched more As and Cd, whereas higher enrichment levels were observed in Phragmites australis leaves, which indicated different transfer capacities of these two wetland plants. The transfer factors for stems and leaves of Phragmites australis in the tidal flooding wetlands significantly differed from those in the short-term flooding wetlands, however, no significant differences in transfer factors for stems and leaves of Suaeda salsa were observed between these two types of wetlands.

  12. Moving from a regional to a continental perspective of Phragmites australis invasion in North America

    Science.gov (United States)

    Kettenring, Karin M.; de Blois, Sylvie; Hauber, Donald P.

    2012-01-01

    Aims We use a regional comparison of Phragmites australis (common reed) subsp. americanus, P. australis subsp. berlandieri and introduced P. australis (possibly five sublineages) in the Chesapeake Bay, the St Lawrence River, Utah and the Gulf Coast to inform a North American perspective on P. australis invasion patterns, drivers, impacts and research needs. Findings and research needs Our regional assessments reveal substantial diversity within and between the three main lineages of P. australis in terms of mode of reproduction and the types of environment occupied. For introduced P. australis, the timing of introduction also differed between the regions. Nevertheless, a common finding in these regions reinforces the notion that introduced P. australis is opportunistic and thrives in disturbed habitats. Thus, we expect to see substantial expansion of introduced P. australis with increasing anthropogenic disturbances in each of these regions. Although there have been some studies documenting the negative impacts of introduced P. australis, it also plays a beneficial role in some regions, and in some cases, the purported negative impacts are unproven. There is also a broader need to clarify the genetic and ecological relationships between the different introduced sublineages observed in North America, and their relative competitive ability and potential for admixture. This may be done through regional studies that use similar methodologies and share results to uncover common patterns and processes. To our knowledge, such studies have not been performed on P. australis in spite of the broad attention given to this species. Such research could advance theoretical knowledge on biological invasion by helping to determine the extent to which the patterns observed can be generalized or are sublineage specific or region specific. Synthesis Given what appears to be sometimes idiosyncratic invasion patterns when interpreted in isolation in the regions that we analysed, it may

  13. Development of anatomical structure of roots of Phragmites australis

    Czech Academy of Sciences Publication Activity Database

    Soukup, A.; Votrubová, O.; Čížková, Hana

    2002-01-01

    Roč. 153, - (2002), s. 277-287 ISSN 0028-646X R&D Projects: GA MŠk OC 844.20; GA MŠk VS96145 Institutional research plan: CEZ:AV0Z6005908 Keywords : Casparian bands * exodermis * endodermis * lignin * suberin * root anatomy * Phragmites Subject RIV: EF - Botanics Impact factor: 2.945, year: 2002

  14. The effects of litter on growth and plasticity of Phragmites australis clones originating from infertile, fertile or eutrophicated habitats

    NARCIS (Netherlands)

    Clevering, O.A.

    1999-01-01

    In many European countries a strong decline of Phragmites australis (Cav.) Trin. Ex Steudel (common reed) has been observed. In some instances this decline has been related to the accumulation of litter. A greenhouse experiment was conducted with P. australis cuttings from different stable and

  15. Quantification of environment-driven changes in epiphytic macroinvertebrate communities associated to Phragmites australis

    OpenAIRE

    Miguel CAÑEDO-ARGÜELLES; Maria RIERADEVALL

    2009-01-01

    The epiphytic macroinvertebrate communities associated with the Common Reed, Phragmites australis (Cav.) Trin. ex Steudel, were examined seasonally from summer 2004 to spring 2005 in eleven coastal lagoons of the Llobregat Delta (NE Spain) following the method proposed by Kornijów & Kairesalo (1994). The aims of the study were to: 1) characterise and quantify changes in epiphytic macroinvertebrate communities along environmental gradients; 2) assess the contribution of elements of the epi...

  16. Allelopathy and resource competition: the effects of Phragmites australis invasion in plant communities

    OpenAIRE

    Uddin, Md Nazim; Robinson, Randall William

    2017-01-01

    Background Phragmites australis, a ubiquitous wetland plant, has been considered one of the most invasive species in the world. Allelopathy appears to be one of the invasion mechanisms, however, the effects could be masked by resource competition among target plants. The difficulty of distinguishing allelopathy from resource competition among plants has hindered investigations of the role of phytotoxic allelochemicals in plant communities. This has been addressed via experiments conducted in ...

  17. Phragmites australis root secreted phytotoxin undergoes photo-degradation to execute severe phytotoxicity.

    Science.gov (United States)

    Rudrappa, Thimmaraju; Choi, Yong Seok; Levia, Delphis F; Legates, David R; Lee, Kelvin H; Bais, Harsh P

    2009-06-01

    Our study organism, Phragmites australis (common reed), is a unique invader in that both native and introduced lineages are found coexisting in North America. This allows one to make direct assessments of physiological differences between these different subspecies and examine how this relates to invasiveness. Recent efforts to understand plant invasive behavior show that some invasive plants secrete a phytotoxin to ward-off encroachment by neighboring plants (allelopathy) and thus provide the invaders with a competitive edge in a given habitat. Here we show that a varying climatic factor like ultraviolet (UV) light leads to photo-degradation of secreted phytotoxin (gallic acid) in P. australis rhizosphere inducing higher mortality of susceptible seedlings. The photo-degraded product of gallic acid (hereafter GA), identified as mesoxalic acid (hereafter MOA), triggered a similar cell death cascade in susceptible seedlings as observed previously with GA. Further, we detected the biological concentrations of MOA in the natural stands of exotic and native P. australis. Our studies also show that the UV degradation of GA is facilitated at an alkaline pH, suggesting that the natural habitat of P. australis may facilitate the photo-degradation of GA. The study highlights the persistence of the photo-degraded phytotoxin in the P. australis's rhizosphere and its inhibitory effects against the native plants.

  18. Surveying managers to inform a regionally relevant invasive Phragmites australis control research program.

    Science.gov (United States)

    Rohal, C B; Kettenring, K M; Sims, K; Hazelton, E L G; Ma, Z

    2018-01-15

    Managers of invasive species consider the peer-reviewed literature only moderately helpful for guiding their management programs. Though this "knowing-doing gap" has been well-described, there have been few efforts to guide scientists in how to develop useful and usable science. Here we demonstrate how a comprehensive survey of managers (representing 42 wetland management units across the Great Salt Lake watershed) can highlight management practices and challenges (here for the widespread invasive plant, Phragmites australis, a recent and aggressive invader in this region) to ultimately inform a research program. The diversity of surveyed organizations had wide-ranging amounts of Phragmites which led to different goals and approaches including more aggressive control targets and a wider array of control tools for smaller, private organizations compared to larger government-run properties. We found that nearly all managers (97%) used herbicide as their primary Phragmites control tool, while burning (65%), livestock grazing (49%), and mowing (43%) were also frequently used. Managers expressed uncertainties regarding the timing of herbicide application and type of herbicide for effective control. Trade-offs between different Phragmites treatments were driven by budgetary concerns, as well as environmental conditions like water levels and social constraints like permitting issues. Managers had specific ideas about the plant communities they desired following Phragmites control, yet revegetation of native species was rarely attempted. The results of this survey informed the development of large-scale, multi-year Phragmites control and native plant revegetation experiments to address management uncertainties regarding herbicide type and timing. The survey also facilitated initial scientist-manager communication, which led to collaborations and knowledge co-production between managers and researchers. An important outcome of the survey was that experimental results were

  19. Cosmopolitan Species As Models for Ecophysiological Responses to Global Change: The Common Reed Phragmites australis

    Directory of Open Access Journals (Sweden)

    Franziska Eller

    2017-11-01

    Full Text Available Phragmites australis is a cosmopolitan grass and often the dominant species in the ecosystems it inhabits. Due to high intraspecific diversity and phenotypic plasticity, P. australis has an extensive ecological amplitude and a great capacity to acclimate to adverse environmental conditions; it can therefore offer valuable insights into plant responses to global change. Here we review the ecology and ecophysiology of prominent P. australis lineages and their responses to multiple forms of global change. Key findings of our review are that: (1 P. australis lineages are well-adapted to regions of their phylogeographic origin and therefore respond differently to changes in climatic conditions such as temperature or atmospheric CO2; (2 each lineage consists of populations that may occur in geographically different habitats and contain multiple genotypes; (3 the phenotypic plasticity of functional and fitness-related traits of a genotype determine the responses to global change factors; (4 genotypes with high plasticity to environmental drivers may acclimate or even vastly expand their ranges, genotypes of medium plasticity must acclimate or experience range-shifts, and those with low plasticity may face local extinction; (5 responses to ancillary types of global change, like shifting levels of soil salinity, flooding, and drought, are not consistent within lineages and depend on adaptation of individual genotypes. These patterns suggest that the diverse lineages of P. australis will undergo intense selective pressure in the face of global change such that the distributions and interactions of co-occurring lineages, as well as those of genotypes within-lineages, are very likely to be altered. We propose that the strong latitudinal clines within and between P. australis lineages can be a useful tool for predicting plant responses to climate change in general and present a conceptual framework for using P. australis lineages to predict plant responses

  20. [Allelopathic interactions between invasive plant Solidago canadensis and native plant Phragmites australis].

    Science.gov (United States)

    Li, Yu-Zhe; Fan, Jiang-Wen; Yin, Xin; Yang, En-Yi; Wei, Wei; Tian, Zhi-Hui; Da, Liang-Jun

    2011-05-01

    Taking the seeds of invasive plant Solidago canadensis and native plant Phragmites australis from their mono- and co-dominant communities as allelopathic acceptors, this paper analyzed the differences in the seed germination rate and sprout length after treated with five level (12.5, 25, 50, 100, and 200 mg x mL(-1)) S. canadensis and P. australis extracts, aimed to understand the allelopathic interactions between the two species. The 1000-grain weight and seed germination rate under distilled water treatment of the two species in co-dominated community were greater than those in mono-dominant community. Low level (12.5 and 25 mg x mL(-1)) S. canadensi extracts slightly promoted the seed germination rates of S. canadensis in both mono- and co-dominant communities, but high level (50, 100, and 200 mg x mL(-1)) S. canadensi extracts had strong inhibition effect, especially for the S. canadensis in co-dominated community. No significant patterns were observed about the effects of P. australis extract on S. canadensis seed germination. The sprout length of S. canadensis seeds in both mono- and co-dominant communities decreased with increasing level of S. canadensis extract, but decreased in a fluctuation way with increasing level of P. australis extract. After treated with the extracts of P. australis or S. canadensis, the seed germination rate of P. australis in mono-dominant community was significantly greater than that in co-dominant community (P < 0.05), but there was no significant difference between these two extracts.

  1. The Effect of Artificial Mowing on the Competition of Phragmites australis and Spartina alterniflora in the Yangtze Estuary

    Directory of Open Access Journals (Sweden)

    Yue Yuan

    2017-01-01

    Full Text Available Spartina alterniflora Loisel. is one of the most invasive species in the world. However, little is known about the role of artificial mowing in its invasiveness and competiveness. In this work, we studied the effect of mowing on its interspecific interactions with native species Phragmites australis (Cav. Trin ex Steud of the Yangtze Estuary, China. We calculated their relative neighbor effect (RNE index, effect of relative crowding (Dr index, and interaction strength (I index. The results showed that the RNE of Phragmites australis and Spartina alterniflora was 0.354 and 0.619, respectively, and they have competitive interactions. The mowing treatments can significantly influence the RNE of Phragmites australis and Spartina alterniflora on each other. Concretely, the RNE of Spartina alterniflora in the removal treatments was significantly higher than the value in the controls. But the RNE of Phragmites australis in the removal treatments was significantly lower than the value in the controls. Meanwhile, Dr of the two species on the targets was higher in the removal treatments than that in the controls, and the opposite was for I. We concluded that artificial mowing could promote the invasion of Spartina alterniflora by increasing its competitive performance compared with native species.

  2. Role of Phragmites australis (common reed) for heavy metals phytoremediation of estuarine sediments.

    Science.gov (United States)

    Cicero-Fernández, Diego; Peña-Fernández, Manuel; Expósito-Camargo, Jose A; Antizar-Ladislao, Blanca

    2016-01-01

    The ability of Phragmites australis to take up heavy metals (Co, Ni, Mo, Cd, Pb, Cr, Cu, Fe, Mn, Zn, and Hg) and other trace elements (As, Se, Ba), from estuarine sediments was investigated using a pilot plant experimental approach. Bioaccumulation (BCF) and translocation factors (TF) were calculated in vegetative and senescence periods for two populations of P. australis, from contaminated (MIC) and non-contaminated (GAL) estuarine sediments, respectively, both growing in estuarine contaminated sediment (RIA) from ría del Carmen y Boo, Santander Bay, Spain. The highest BCF values were obtained for Ni (0.43), Ba (0.43) Mo (0.36), Cr (0.35), and Cd (0.31) for plants collected from site GAL following the senescence period. The highest BCF values recorded for plants collected from MIC following the senescence period were for Mo (0.22) and Cu (0.22). Following senescence, plants collected from GAL and MIC presented TF>1 for Ni, Mo, Se, and Zn, and in addition plants collected from MIC presented TF>1 for Ba, Cr, and Mn. A substantial increase of Micedo's rhizosphere, six times higher than Galizano's rhizosphere, suggested adaptation to contaminated sediment. The evaluated communities of P. australis demonstrated their suitability for phytoremediation of heavy metals contaminated estuarine sediments.

  3. Decomposition of Phragmites australis litter retarded by invasive Solidago canadensis in mixtures: an antagonistic non-additive effect

    Science.gov (United States)

    Zhang, Ling; Zhang, Yaojun; Zou, Jianwen; Siemann, Evan

    2014-06-01

    Solidago canadensis is an aggressive invader in China. Solidago invasion success is partially attributed to allelopathic compounds release and more benefits from AM fungi, which potentially makes the properties of Solidago litter different from co-occurring natives. These properties may comprehensively affect litter decomposition of co-occurring natives. We conducted a field experiment to examine litter mixing effects in a Phragmites australis dominated community invaded by Solidago in southeast China. Solidago had more rapid mass and N loss rate than Phragmites when they decomposed separately. Litter mixing decreased N loss rate in Phragmites litter and increased that of Solidago. Large decreases in Phragmites mass loss and smaller increases in Solidago mass loss caused negative non-additive effect. Solidago litter extracts reduced soil C decomposition and N processes, suggested an inhibitory effect of Solidago secondary compounds. These results are consistent with the idea that nutrient transfer and secondary compounds both affected litter mixtures decomposition.

  4. Mapping invasive Phragmites australis in the coastal Great Lakes with ALOS PALSAR satellite imagery for decision support

    Science.gov (United States)

    Bourgeau-Chavez, Laura L.; Kowalski, Kurt P.; Carlson Mazur, Martha L.; Scarbrough, Kirk A.; Powell, Richard B.; Brooks, Colin N.; Huberty, Brian; Jenkins, Liza K.; Banda, Elizabeth C.; Galbraith, David M.; Laubach, Zachary M.; Riordan, Kevin

    2013-01-01

    The invasive variety of Phragmites australis (common reed) forms dense stands that can cause negative impacts on coastal Great Lakes wetlands including habitat degradation and reduced biological diversity. Early treatment is key to controlling Phragmites, therefore a map of the current distribution is needed. ALOS PALSAR imagery was used to produce the first basin-wide distribution map showing the extent of large, dense invasive Phragmites-dominated habitats in wetlands and other coastal ecosystems along the U.S. shore of the Great Lakes. PALSAR is a satellite imaging radar sensor that is sensitive to differences in plant biomass and inundation patterns, allowing for the detection and delineation of these tall (up to 5 m), high density, high biomass invasive Phragmites stands. Classification was based on multi-season ALOS PALSAR L-band (23 cm wavelength) HH and HV polarization data. Seasonal (spring, summer, and fall) datasets were used to improve discrimination of Phragmites by taking advantage of phenological changes in vegetation and inundation patterns over the seasons. Extensive field collections of training and randomly selected validation data were conducted in 2010–2011 to aid in mapping and for accuracy assessments. Overall basin-wide map accuracy was 87%, with 86% producer's accuracy and 43% user's accuracy for invasive Phragmites. The invasive Phragmites maps are being used to identify major environmental drivers of this invader's distribution, to assess areas vulnerable to new invasion, and to provide information to regional stakeholders through a decision support tool.

  5. Phragmites australis

    DEFF Research Database (Denmark)

    Bui, Truong Tho; Sorrell, Brian K.; Lambertini, Carla

    2016-01-01

    It has been suggested that in plant invasions, species may develop intrinsically higher gas exchange and growth rates, and greater nitrogen uptake and allocation to shoots, in their invasive range than in their native habitat under excess nutrients. In this study, native populations of two old...... world Phragmitesaustralis phylogeographic groups (EU and MED) were compared with their invasive populations in North America [NAint (M) and NAint (Delta)] under unlimited nutrient availability and identical environmental conditions in a common garden. We expected that both introduced groups would have......, the introduced groups invested more biomass in above-ground tissues than roots and rhizomes. Our results support the concept that invasive populations develop enhanced morphological, physiological and biomass traits in their new ranges that may assist their competiveness under nutrient-enriched conditions...

  6. Preparation and Adsorption Performances of Phragmites australis Activated Carbon with High Acidity

    Directory of Open Access Journals (Sweden)

    FU Cheng-kai

    2017-03-01

    Full Text Available For removal of heavy metals from wastewater and recycling the wetland plants, the present study investigated the viability of using silage of Phragmites australis (PA to prepare activated carbons (ACs with high acidity. BET surface area, porous texture and surface functional characteristics of ACs were analyzed by N2 adsorption/desorption, elemental analysis and Boehm titration method. ACs presented well-developed micro-porosity and favorable surface acidity. The sorption equilibrium data for Ni (Ⅱ and Cd (Ⅱ sorption onto ACs were analyzed by the Langmuir and Freundlich models. The Langmuir model was fitted well to the adsorption behavior. The properties of high surface acidity promoted the adsorption of heavy metals by the silage-treated ACs and the chemical sorption played the key role in the sorption process.

  7. Uptake of Carbamazepine by rhizomes and endophytic bacteria of Phragmites australis

    Directory of Open Access Journals (Sweden)

    Andres eSauvetre

    2015-02-01

    Full Text Available Carbamazepine is an antiepileptic and mood-stabilizing drug which is used widely in Europe and North America. In the environment, it is found as a persistent and recalcitrant conta¬mi-nant, being one of the most prominent hazardous pharmaceuticals and personal care products (PPCPs in effluents of wastewater treatment plants (WWTPs. Phragmites australis is one of the species with both, the highest potential of detoxification and phytoremediation. It has been used successfully in the treatment of industrial and municipal wastewater. Recently, the identification of endophytic micro¬organisms from different plant species growing in contaminated sites has provided a list of candidates which could be used as bio-inoculants for bioremediation of difficult compounds. In this study, Phragmites australis plants were exposed to 5 mg/L of carbamazepine. After 9 days the plants had removed 90% of the initial concentration. Endophytic bacteria were isolated from these plants and further characterized. Phylogenetic analysis based on 16S rDNA sequencing revealed that the majority of these isolates belong to three groups: Proteobacteria, Actinobacteria and Bacteroidetes. Carbamazepine uptake and plant growth promoting (PGP traits were analyzed among the isolates. Ninety percent of the isolates produce indole acetic acid (IAA and all of them possess at least one of the PGP traits tested. One isolate identified as Chryseobacterium taeanense combines good carbamazepine uptake and all of the PGP traits. Rhizobium daejeonense can remove carbamazepine and produces 23 µg/mL of IAA. Diaphorobacter nitroreducens and Achromobacter mucicolens are suitable for carbamazepine removal while both, Pseudomonas veronii and Pseudomonas lini show high siderophore production and phosphate solubilization. Alone or in combination, these isolates might be applied as inoculates in constructed wetlands in order to enhance the phyto-remediation of carbamazepine during wastewater

  8. Diversity of fungal endophytes in non-native Phragmites australis in the Great Lakes

    Science.gov (United States)

    Clay, Keith; Shearin, Zachery; Bourke, Kimberly; Bickford, Wesley A.; Kowalski, Kurt P.

    2016-01-01

    Plant–microbial interactions may play a key role in plant invasions. One common microbial interaction takes place between plants and fungal endophytes when fungi asymptomatically colonize host plant tissues. The objectives of this study were to isolate and sequence fungal endophytes colonizing non-native Phragmites australis in the Great Lakes region to evaluate variation in endophyte community composition among three host tissue types and three geographical regions. We collected entire ramets from multiple clones and populations, surface sterilized plant tissues, and plated replicate tissue samples from leaves, stems, and rhizomes on corn meal agar plates to culture and isolate fungal endophytes. Isolates were then subjected to Sanger sequencing of the ITS region of the nuclear ribosomal DNA. Sequences were compared to fungal databases to define operational taxonomic units (OTUs) that were analyzed statistically for community composition. In total, we obtained 173 endophyte isolates corresponding to 55 OTUs, 39 of which were isolated only a single time. The most common OTU corresponded most closely to Sarocladium strictum and comprised 25 % of all fungal isolates. More OTUs were found in stem tissues, but endophyte diversity was greatest in rhizome tissues. PERMANOVA analyses indicated significant differences in endophyte communities among tissue types, geographical regions, and the interaction between those factors, but no differences among individual ramets were detected. The functional role of the isolated endophytes is not yet known, but one genus isolated here (Stagonospora) has been reported to enhance Phragmites growth. Understanding the diversity and functions of Phragmites endophytes may provide targets for control measures based on disrupting host plant/endophyte interactions.

  9. Bioavailability of cadmium adsorbed on various oxides minerals to wetland plant species Phragmites australis

    International Nuclear Information System (INIS)

    Wang He; Jia Yongfeng; Wang Shaofeng; Zhu Huijie; Wu Xing

    2009-01-01

    The bioavailability of heavy metals strongly depends on their speciation in the environment. The effect of different chemical speciations of cadmium ions (i.e. adsorbed on different oxide minerals) on its bioavailability to wetland plant Phragmites australis was studied. Goethite, magnetite, gibbsite, alumina, and manganese oxide were chosen as representatives of metal (hydr)oxides commonly present in sediment. The cultivar system with Hoagland solution as nutrition supply, and single metal oxide with adsorbed Cd as contaminant was applied to study Cd accumulation by P. australis. The bioaccumulation degree in root after the 45-day treatment followed the order: Al(OH) 3 > Al 2 O 3 > Fe 3 O 4 > MnO 2 > FeOOH. The concentration of Cd in stem and leaf followed a similar order although it was considerably lower than that in root. Low-molecular-weight organic acids (LMWOAs), acetic acid, malic acid and citric acid were used to evaluate the desorbability of Cd from different oxides, which can be indicative of Cd-oxide bonding strength and Cd bioavailability. Desorption of Cd by acetic acid and malic acid followed the order: Al(OH) 3 > Fe 3 O 4 > Al 2 O 3 > FeOOH > MnO 2 , while by citric acid: Al(OH) 3 ≥ Al 2 O 3 > Fe 3 O 4 > FeOOH > MnO 2 . This was consistent with the Cd accumulation degree in the plant. Cd adsorbed on Al(OH) 3 was the most easily desorbable species and most bioavailable to P. australis among the oxide minerals, whereas MnO 2 adsorbed Cd was least desorbable by LMWOAs hence constituted the least bioavailable Cd species adsorbed on the oxide minerals.

  10. Phytoextraction of chloride from a cement kiln dust (CKD) contaminated landfill with Phragmites australis.

    Science.gov (United States)

    McSorley, Kaitlin; Rutter, Allison; Cumming, Robert; Zeeb, Barbara A

    2016-05-01

    Cement kiln dust (CKD) is a globally produced by-product from cement manufacturing that is stockpiled or landfilled. Elevated concentrations of chloride pose toxic threats to plants and aquatic communities, as the anion is highly mobile in water and can leach into surrounding water sources. Re-vegetation and in situ phytoextraction of chloride from a CKD landfill in Bath, ON, Canada, was investigated with the resident invasive species Phragmites australis (haplotype M). Existing stands of P. australis were transplanted from the perimeter of the site into the highest areas of contamination (5.9×10(3)μg/g). Accumulation in the shoots of P. australis was quantified over one growing season by collecting samples from the site on a bi-weekly basis and analyzing for chloride. Concentrations decreased significantly from early May (24±2.2×10(3)μg/g) until mid-June (15±2.5×10(3)μg/g), and then remained stable from June to August. Shoot chloride accumulation was not significantly affected by water level fluctuations at the site, however elevated potassium concentrations in the soil may have contributed to uptake. Based on shoot chloride accumulation and total biomass, it was determined that phytoextraction from the CKD landfill can remove 65±4kg/km(2) of chloride per season. Based on this extraction rate, removal of chloride present in the highly contaminated top 10cm of soil can be achieved in 3-9years. This is the first study to apply phytotechnologies at a CKD landfill, and to successfully demonstrate in situ phytoextraction of chloride. Copyright © 2015 Elsevier Ltd. All rights reserved.

  11. Isolation, cloning, and characterization of a partial novel aro A gene in common reed (Phragmites australis).

    Science.gov (United States)

    Taravat, Elham; Zebarjadi, Alireza; Kahrizi, Danial; Yari, Kheirollah

    2015-05-01

    Among the essential amino acids, phenylalanine, tryptophan, and tyrosine are aromatic amino acids which are synthesized by the shikimate pathway in plants and bacteria. Herbicide glyphosate can inhibit the biosynthesis of these amino acids. So, identification of the gene tolerant to glyphosate is very important. It has been shown that the common reed or Phragmites australis Cav. (Poaceae) is relatively tolerant to glyphosate. The aim of the current research is identification, cloning, sequencing, and registering of partial aro A gene of the common reed P. australis. The partial aro A gene of common reed (P. australis) was cloned in Escherichia coli and the amino acid sequence was identified/determined for the first time. This is the first report for isolation, cloning, and sequencing of a part of aro A gene from the common reed. A 670 bp fragment including two introns (86 bp and 289 bp) was obtained. The open reading frame (ORF) region in part of gene was encoded for 98 amino acids. Alignment showed high similarity among this region with Zea mays (L.) (Poaceae) (94.6%), Eleusine indica L. Gaertn (Poaceae) (94.2%), and Zoysia japonica Steud. (Poaceae) (94.2%). The alignment of amino acid sequence of the investigated part of the gene showed a homology with aro A from several other plants. This conserved region forms the enzyme active site. The alignment results of nucleotide and amino acid residues with related sequences showed that there are some differences among them. The relative glyphosate tolerance in the common reed may be related to these differences.

  12. Bioavailability of cadmium adsorbed on various oxides minerals to wetland plant species Phragmites australis

    Energy Technology Data Exchange (ETDEWEB)

    Wang He, E-mail: he.wangworld@yahoo.com.cn [Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, No. 72 Wenhua Road, Shenyang 110016 (China); Graduate School, Chinese Academy of Sciences, Beijing 100049 (China); Jia Yongfeng, E-mail: yongfeng.jia@iae.ac.cn [Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, No. 72 Wenhua Road, Shenyang 110016 (China); Wang Shaofeng [Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, No. 72 Wenhua Road, Shenyang 110016 (China); Zhu Huijie; Wu Xing [Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, No. 72 Wenhua Road, Shenyang 110016 (China); Graduate School, Chinese Academy of Sciences, Beijing 100049 (China)

    2009-08-15

    The bioavailability of heavy metals strongly depends on their speciation in the environment. The effect of different chemical speciations of cadmium ions (i.e. adsorbed on different oxide minerals) on its bioavailability to wetland plant Phragmites australis was studied. Goethite, magnetite, gibbsite, alumina, and manganese oxide were chosen as representatives of metal (hydr)oxides commonly present in sediment. The cultivar system with Hoagland solution as nutrition supply, and single metal oxide with adsorbed Cd as contaminant was applied to study Cd accumulation by P. australis. The bioaccumulation degree in root after the 45-day treatment followed the order: Al(OH){sub 3} > Al{sub 2}O{sub 3} > Fe{sub 3}O{sub 4} > MnO{sub 2} > FeOOH. The concentration of Cd in stem and leaf followed a similar order although it was considerably lower than that in root. Low-molecular-weight organic acids (LMWOAs), acetic acid, malic acid and citric acid were used to evaluate the desorbability of Cd from different oxides, which can be indicative of Cd-oxide bonding strength and Cd bioavailability. Desorption of Cd by acetic acid and malic acid followed the order: Al(OH){sub 3} > Fe{sub 3}O{sub 4} > Al{sub 2}O{sub 3} > FeOOH > MnO{sub 2}, while by citric acid: Al(OH){sub 3} {>=} Al{sub 2}O{sub 3} > Fe{sub 3}O{sub 4} > FeOOH > MnO{sub 2}. This was consistent with the Cd accumulation degree in the plant. Cd adsorbed on Al(OH){sub 3} was the most easily desorbable species and most bioavailable to P. australis among the oxide minerals, whereas MnO{sub 2} adsorbed Cd was least desorbable by LMWOAs hence constituted the least bioavailable Cd species adsorbed on the oxide minerals.

  13. Allelopathy and resource competition: the effects of Phragmites australis invasion in plant communities.

    Science.gov (United States)

    Uddin, Md Nazim; Robinson, Randall William

    2017-12-01

    Phragmites australis, a ubiquitous wetland plant, has been considered one of the most invasive species in the world. Allelopathy appears to be one of the invasion mechanisms, however, the effects could be masked by resource competition among target plants. The difficulty of distinguishing allelopathy from resource competition among plants has hindered investigations of the role of phytotoxic allelochemicals in plant communities. This has been addressed via experiments conducted in both the greenhouse and laboratory by growing associated plants, Melaleuca ericifolia, Rumex conglomeratus, and model plant, Lactuca sativa at varying densities with the allelopathic plant, P. australis, its litter and leachate of P. australis litter. This study investigated the potential interacting influences of allelopathy and resource competition on plant growth-density relationships. In greenhouse, the root exudates mediated effects showed the strongest growth inhibition of M. ericifolia at high density whereas litter mediated results revealed increased growth at medium density treatments compared to low and high density. Again, laboratory experiments related to seed germination and seedling growth of L. sativa and R. conglomeratus exhibited phytotoxicity decreased showing positive growth as plant density increased and vice versa. Overall, the differential effects were observed among experiments but maximum individual plant biomass and some other positive effects on plant traits such as root and shoot length, chlorophyll content occurred at an intermediate density. This was attributed to the sharing of the available phytotoxin among plants at high densities which is compatible to density-dependent phytotoxicity model. The results demonstrated that plant-plant interference is the combined effect of allelopathy and resource competition with many other factors but this experimental design, target-neighbor mixed-culture in combination of plant grown at varying densities with varying

  14. Seasonal changes in metal accumulation and distribution in the organs of Phragmites australis (common reed from Lake Skadar, Montenegro

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    Kastratović Vlatko

    2013-01-01

    Full Text Available Due to its ability to accumulate metals, availability throughout the year and its large biomass, Phragmites australis (common reed is suitable for biomonitoring studies for the evaluation of load level of water ecosystem with trace metals. The heavy metals concentration in P.australis tissue can be several ten to several thousand times higher than those in the surrounding water. In this study we examined the content of heavy metals (Cd, Co, Cr, Cu, Mn, Ni, Pb, Zn, Sr and V in sediment, water and different organs of Phragmites australis collected from Lake Skadar, Montenegro, during different seasons of the year 2011. The highest concentrations of Sr were found in the leaves, while the other studied metals showed their highest concentrations in the roots. Thus, P. australis is considered a root bioaccumulation species. For most metals the concentration in roots and stems increases over time until the end of the growing season, and then decreases, while the concentration in leaves increases even after the growing season of the plant. If P. australis is used for phytoremediation purposes, then it should be harvested after the growing season because then the concentration of metals in the aboveground parts is maximal.

  15. Decomposition of Phragmites australis litter retarded by invasive Solidago canadensis in mixtures: an antagonistic non-additive effect

    OpenAIRE

    Zhang, Ling; Zhang, Yaojun; Zou, Jianwen; Siemann, Evan

    2014-01-01

    Solidago canadensis is an aggressive invader in China. Solidago invasion success is partially attributed to allelopathic compounds release and more benefits from AM fungi, which potentially makes the properties of Solidago litter different from co-occurring natives. These properties may comprehensively affect litter decomposition of co-occurring natives. We conducted a field experiment to examine litter mixing effects in a Phragmites australis dominated community invaded by Solidago in southe...

  16. CO2 and CH4 exchange by Phragmites australis under different climates

    Science.gov (United States)

    Serrano Ortiz, Penélope; Chojnickic, Bogdan H.; Sánchez-Cañete, Enrique P.; Kowalska, Natalia; López-Ballesteros, Ana; Fernández, Néstor; Urbaniak, Marek; Olejnik, Janusz; Kowalski, Andrew S.

    2015-04-01

    The key role of wetlands regarding global warming is the resulting balance between net CO2 assimilation, via photosynthesis, and CO2 and CH4 emissions, given the potential to release stored carbon, because of the high temperature sensitivity of heterotrophic soil respiration and anoxic conditions. However, it is still unknown whether wetlands will convert from long-term carbon sinks to sources as a result of climate change and other anthropogenic effects such as land use changes. Phragmites australis is one of the most common species found in wetlands and is considered the most globally widespread and productive plant species in this type of ecosystem. In this context, the main objective of this study is to analyse the GHG exchange (CO2 and CH4) of two wetlands with Phragmites australis as the dominant species under different climates using the eddy covariance (EC) technique. The first site, Padul, is located in southern Spain, with a sub-humid warm climate, characterised by a mean annual temperature of 16°C and annual precipitation of ca. 470 mm, with a very dry summer. The second site, Rzecin is located in Poland with a mean annual temperature of 8°C, and annual precipitation around 600mm with no dry season. The Padul EC station is equipped with two infrared gas analysers to measure CO2 and CH4 fluxes (LI-7200 and LI-7700 respectively) while the Rzecin EC station has the same CH4 sensor as Padul, but also a sensor measuring both GHG fluxes (DLT-100 Fast Methane Analyser, Los Gatos). In this study, we present: i) the results of a CH4 analyser inter-comparison campaign (LI-7700 vs. Los Gatos), ii) a comparative analysis of the functional behaviour of respiration and photosynthesis in both sites testing relationships between CO2 fluxes measured with the EC technique and meteorological variables such as temperature and direct or diffuse radiation and iii) the CH4 dynamicsat both sites by identifying, when possible, annual, seasonal and diurnal patterns.

  17. Seasonal changes of metal accumulation and distribution in common club rush (Schoenoplectus lacustris) and common reed (Phragmites australis).

    Science.gov (United States)

    Duman, Fatih; Cicek, Mehmet; Sezen, Goksal

    2007-08-01

    In this study, two aquatic macrophytes Phragmites australis and Schoenoplectus lacustris and corresponding sediment samples were collected every three months from Lake Sapanca (Turkey) and analysed for their heavy-metal contents (Pb, Cr, Cu, Mn, Ni, Zn and Cd). Accumulation factor ratios of plant parts were calculated for all metals, and the two species were compared in terms of accumulation properties. The highest concentrations were measured in the root systems while relatively low concentrations were found in the rhizome and above-ground parts of the plants. The accumulation ratios of root for P. australis were usually higher than the ratios for S. lacustris. While the accumulation ratios of root were higher in winter than in the other seasons for P. australis, for S. lacustris the highest accumulation ratios were found in the autumn. Both plant species were found to be root accumulators of Pb, Cu, Mn, Ni, Zn and Cd.

  18. Decomposition of Phragmites australis rhizomes in artificial land-water transitional zones (ALWTZs) and management implications

    Science.gov (United States)

    Han, Zhen; Cui, Baoshan; Zhang, Yongtao

    2015-09-01

    Rhizomes are essential organs for growth and expansion of Phragmites australis. They function as an important source of organic matter and as a nutrient source, especially in the artificial land-water transitional zones (ALWTZs) of shallow lakes. In this study, decomposition experiments on 1- to 6-year-old P. australis rhizomes were conducted in the ALWTZ of Lake Baiyangdian to evaluate the contribution of the rhizomes to organic matter accumulation and nutrient release. Mass loss and changes in nutrient content were measured after 3, 7, 15, 30, 60, 90, 120, and 180 days. The decomposition process was modeled with a composite exponential model. The Pearson correlation analysis was used to analyze the relationships between mass loss and litter quality factors. A multiple stepwise regression model was utilized to determine the dominant factors that affect mass loss. Results showed that the decomposition rates in water were significantly higher than those in soil for 1- to 6-year-old rhizomes. However, the sequence of decomposition rates was identical in both water and soil. Significant relationships between mass loss and litter quality factors were observed at a later stage, and P-related factors proved to have a more significant impact than N-related factors on mass loss. According to multiple stepwise models, the C/P ratio was found to be the dominant factor affecting the mass loss in water, and the C/N and C/P ratios were the main factors affecting the mass loss in soil. The combined effects of harvesting, ditch broadening, and control of water depth should be considered for lake administrators.

  19. Disease protection and allelopathic interactions of seed-transmitted endophytic pseudomonads of invasive reed grass (Phragmites australis)

    Science.gov (United States)

    White, James F.; Kingsley, Katheryn I; Kowalski, Kurt P.; Irizarry, Ivelisse; Micci, April; Soares, Marcos Antonio; Bergen, Marshall S.

    2018-01-01

    Background and aimsNon-native Phragmites australis (haplotype M) is an invasive grass that decreases biodiversity and produces dense stands. We hypothesized that seeds of Phragmites carry microbes that improve seedling growth, defend against pathogens and maximize capacity of seedlings to compete with other plants.MethodsWe isolated bacteria from seeds of Phragmites, then evaluated representatives for their capacities to become intracellular in root cells, and their effects on: 1.) germination rates and seedling growth, 2.) susceptibility to damping-off disease, and 3.) mortality and growth of competitor plant seedlings (dandelion (Taraxacum officionale F. H. Wigg) and curly dock (Rumex crispus L.)).ResultsTen strains (of 23 total) were identified and characterized; seven were identified as Pseudomonas spp. Strains Sandy LB4 (Pseudomonas fluorescens) and West 9 (Pseudomonas sp.) entered root meristems and became intracellular. These bacteria improved seed germination in Phragmites and increased seedling root branching in Poa annua. They increased plant growth and protected plants from damping off disease. Sandy LB4 increased mortality and reduced growth rates in seedlings of dandelion and curly dock.ConclusionsPhragmites plants associate with endophytes to increase growth and disease resistance, and release bacteria into the soil to create an environment that is favorable to their seedlings and less favorable to competitor plants.

  20. Resistance strategies of Phragmites australis (common reed to Pb pollution in flood and drought conditions

    Directory of Open Access Journals (Sweden)

    Na Zhang

    2018-01-01

    Full Text Available Resistance strategies of clonal organs, and parent and offspring shoots of Phragmites australis (common reed to heavy metal pollution in soils are not well known. To clarify the tolerance or resistance strategies in reeds, we conducted a pot experiment with five levels of Pb concentration (0∼4,500 mg kg−1 in flood and drought conditions. Lead toxicity had no inhibitory effect on the number of offspring shoots in flood environment; however, biomass accumulation, and photosynthetic and clonal growth parameters were inhibited in both water environment. At each treatment of Pb concentration, offspring shoots had greater biomass and higher photosynthesis indicators than parent shoots. The lowest Pb allocation was found in rhizomes. More of the Pb transported to above-ground parts tended to accumulate in parent shoots rather than in offspring shoots. Biomass and photosynthesis of offspring shoots, rhizome length, and the number of buds, rhizomes and offspring shoots in the flooded treatment were significantly greater than those in the drought treatment. Our results indicated that the tolerance strategies used by reeds, including higher biomass accumulation and photosynthesis in offspring shoots, low allocation of Pb in rhizomes and offspring shoots, and stable clonal growth, maintained the stability of population propagation and productivity, improving the resistance of reeds to Pb pollution in flood environment. However, the resistance or tolerance was significantly reduced by the synergistic effect of Pb and drought, which significantly inhibited biomass accumulation, photosynthesis, and clonal growth of reeds.

  1. An exploration of common reed (Phragmites australis bioenergy potential in North America

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    R. Vaičekonytė

    2014-10-01

    Full Text Available In North America, reed (Phragmites australis is typically considered to be a weed although it provides important ecosystem services. Small, sparse, patchy or mixed reedbeds are more suitable as habitat for many species than extensive dense reedbeds, whose habitat functions can be enhanced by the selective removal of biomass. We propose that above-ground reed biomass could be harvested for bioenergy, at the same time improving habitat for biodiversity by thinning or fragmenting the more extensive reedbeds. Biofuel pellets manufactured from reeds harvested at Montréal (Canada had moisture content 6.4 %, energy content 16.9 kJ g-1 (dry mass, ash content 3.44 %, and chloride content 1962 ppm. Thus, reed as a material for fuel pellet manufacture is similar to switchgrass (Panicum virgatum, which is commonly cultivated for that purpose and requires higher inputs than harvested wild reed. We discuss these findings in the context of environmental considerations and conclude that the bioenergy potential of reed could most expediently be realised in North America by combining material harvested from the widespread spontaneously occurring reedbeds with organic waste from other sources to create mixed biofuels. However, reeds with high levels of chlorine, sulphur or metals should not be burned to avoid air pollution or equipment damage unless these problems are mitigated by means of appropriate season of harvest, equipment, combustion regime, or use of a mixed feedstock.

  2. The removal of heavy metals by iron mine drainage sludge and Phragmites australis

    Science.gov (United States)

    Hoang Ha, Nguyen Thi; Anh, Bui Thi Kim

    2017-06-01

    This study was conducted to assess the removal of heavy metals from solutions by the combination of modified iron mine drainage sludge (sorbent column) and surface and subsurface flow constructed wetlands using the common reed (Phragmites australis) during 30 days of experiment. The results of this study demonstrated that the average removal rates of Zn, Pb, Mn, and As by sorbent column were 59.0, 55.1, 38.7, and 42.4%, respectively. The decreasing trend of removal rates of metals by sorbent column was obtained during the experiment. The average removal rates of Zn, Pb, Mn, and As by sorbent column-surface constructed wetland were 78.9, 73.5, 91.2, and 80.5%, respectively; those by sorbent column-subsurface flow constructed wetland were 81.7, 81.1, 94.1, and 83.1% which reflected that subsurface flow constructed wetland showed higher removal rate than the surface system. Concentrations of heavy metals in the outlet water were lower than the Vietnamese standard limits regulated for industrial wastewater. The results indicate the feasibility of integration of iron mine drainage sludge and constructed wetlands for wastewater treatment.

  3. Interspecific interactions between Phragmites australis and Spartina alterniflora along a tidal gradient in the Dongtan wetland, Eastern China.

    Directory of Open Access Journals (Sweden)

    Yue Yuan

    Full Text Available The invasive species Spartina alterniora Loisel was introduced to the eastern coast of China in the 1970s and 1980s for the purposes of land reclamation and the prevention of soil erosion. The resulting interspecific competition had an important influence on the distribution of native vegetation, which makes studying the patterns and mechanisms of the interactions between Spartina alterniora Loisel and the native species Phragmites australis (Cav. Trin ex Steud in this region very important. There have been some researches on the interspecific interactions between P. australis and S. alterniora in the Dongtan wetland of Chongming, east China, most of which has focused on the comparison of their physiological characteristics. In this paper, we conducted a neighbor removal experiment along a tidal gradient to evaluate the relative competitive abilities of the two species by calculating their relative neighbor effect (RNE index. We also looked at the influence of environmental stress and disturbance on the competitive abilities of the two species by comparing interaction strength (I among different tidal zones both for P. australis and S. alterniora. Finally, we measured physiological characteristics of the two species to assess the physiological mechanisms behind their different competitive abilities. Both negative and positive interactions were found between P. australis and S. alterniora along the environmental gradient. When the direction of the competitive intensity index for P. australis and S. alterniora was consistent, the competitive or facilitative effect of S. alterniora on P. australis was stronger than that of P. australis on S. alterniora. The interspecific interactions of P. australis and S. alterniora varied with environmental conditions, as well as with the method used, to measure interspecific interactions.

  4. Factors affecting post-control reinvasion by seed of an invasive species, Phragmites australis, in the central Platte River, Nebraska.

    Science.gov (United States)

    Galatowitsch, Susan M.; Larson, Diane L.; Larson, Jennifer L.

    2016-01-01

    Invasive plants, such as Phragmites australis, can profoundly affect channel environments of large rivers by stabilizing sediments and altering water flows. Invasive plant removal is considered necessary where restoration of dynamic channels is needed to provide critical habitat for species of conservation concern. However, these programs are widely reported to be inefficient. Post-control reinvasion is frequent, suggesting increased attention is needed to prevent seed regeneration. To develop more effective responses to this invader in the Central Platte River (Nebraska, USA), we investigated several aspects of Phragmites seed ecology potentially linked to post-control reinvasion, in comparison to other common species: extent of viable seed production, importance of water transport, and regeneration responses to hydrology. We observed that although Phragmites seed does not mature until very late in the ice-free season, populations produce significant amounts of viable seed (>50 % of filled seed). Most seed transported via water in the Platte River are invasive perennial species, although Phragmites abundances are much lower than species such as Lythrum salicaria, Cyperus esculentus and Phalaris arundinacea. Seed regeneration of Phragmites varies greatly depending on hydrology, especially timing of water level changes. Flood events coinciding with the beginning of seedling emergence reduced establishment by as much as 59 % compared to flood events that occurred a few weeks later. Results of these investigations suggest that prevention of seed set (i.e., by removal of flowering culms) should be a priority in vegetation stands not being treated annually. After seeds are in the seedbank, preventing reinvasion using prescribed flooding has a low chance of success given that Phragmites can regenerate in a wide variety of hydrologic microsites.

  5. Quantification of environment-driven changes in epiphytic macroinvertebrate communities associated to Phragmites australis

    Directory of Open Access Journals (Sweden)

    Miguel CAÑEDO-ARGÜELLES

    2009-08-01

    Full Text Available The epiphytic macroinvertebrate communities associated with the Common Reed, Phragmites australis (Cav. Trin. ex Steudel, were examined seasonally from summer 2004 to spring 2005 in eleven coastal lagoons of the Llobregat Delta (NE Spain following the method proposed by Kornijów & Kairesalo (1994. The aims of the study were to: 1 characterise and quantify changes in epiphytic macroinvertebrate communities along environmental gradients; 2 assess the contribution of elements of the epiphytic compartment to structuring the community; 3 define the optima and tolerances of selected epiphytic macroinvertebrate taxa for the most relevant ecological factors responsible for assemblage composition; and 4 identify possible epiphytic species assemblages that would allow a lagoon’s typology to be established, as well as their representative indicator species. Communities showed statistically significant seasonal variation, with two faunal peaks: one in summer, with high chironomid densities, and the other in winter, with high naidid densities. These peaks showed a clear response to the influence of environmental factors. Salinity explained the highest percentage of total variance (36%, while trophic variables (nutrients, phytoplanktonic chlorophyll-a, and total organic carbon and epiphyton biomass (19.2 and 4% of total variance explained, respectively were secondary. Three different epiphytic macroinvertebrate species assemblages could be defined. These assemblages were directly linked to conductivity conditions, which determined the rate of survival of certain taxa, and to the existence of a direct connection with the sea, which permitted the establishment of "brackish-water" species. In spite of the existence of these species assemblages, the species composition and biomass of epiphytic macroinvertebrates and epiphyton differed substantially between lagoons; both elements were subject to changes in the environment, which finally determined the site

  6. Nitrous oxide emissions from Phragmites australis-dominated zones in a shallow lake

    International Nuclear Information System (INIS)

    Yang Zhifeng; Zhao Ying; Xia Xinghui

    2012-01-01

    Nitrous oxide (N 2 O) emissions from Phragmites australis (reed) – dominated zones in Baiyangdian Lake, the largest shallow lake of Northern China, were investigated under different hydrological conditions with mesocosm experiments during the growing season of reeds. The daily and monthly N 2 O emissions were positively correlated with air temperature and the variation of aboveground biomass of reeds (p 2 O emissions from reeds were about 45.8–52.8% of that from the sediments. In terms of the effect of hydrological conditions, N 2 O emissions from the aquatic-terrestrial ecotone were 9.4–26.1% higher than the submerged zone, inferring that the variation of water level would increase N 2 O emissions. The annual N 2 O emission from Baiyangdian Lake was estimated to be about 114.2 t. This study suggested that N 2 O emissions from shallow lakes might be accelerated by the climate change as it has increased air temperature and changed precipitation, causing the variation of water level. - Highlights: ► The daily N 2 O emissions were significant positively correlated with air temperature. ► The monthly N 2 O emissions positively correlated with reed aboveground biomass variations. ► The N 2 O emissions from reeds contributed to 45.8–52.8% of that from the sediment. ► N 2 O emissions from the aquatic-terrestrial ecotone were 9.4–26.1% higher than the submerged zone. ► N 2 O emissions from shallow lakes might be accelerated by the climate change. - The increase of air temperature and water level variation would increase N 2 O emissions, suggesting N 2 O emissions from shallow lakes might be accelerated by climate change.

  7. A comparative study on the uptake and translocation of organochlorines by Phragmites australis

    Energy Technology Data Exchange (ETDEWEB)

    San Miguel, Angélique; Ravanel, Patrick [Laboratoire d’Ecologie Alpine, UMR CNRS n°5553, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 09 (France); Raveton, Muriel, E-mail: muriel.raveton@ujf-grenoble.fr [Laboratoire d’Ecologie Alpine, UMR CNRS n°5553, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 09 (France)

    2013-01-15

    Highlights: ► This study compares uptake/translocation of organochlorine congeners in macrophytes. ► First, root OC uptake was strongly linked with the partitioning/diffusion process. ► With time exposure, bioconcentration increased with OC solubility and volatility. ► Translocation was linked to the combination of water flow and vapor flux transfers. ► The most volatile OCs might be phytovolatilized from foliar surfaces. -- Abstract: Organochlorines (OCs) are persistent chemicals found in various environmental compartments. The differences in the uptake of {sup 14}C-labeled 1,4-dichlorobenzene (DCB), 1,2,4-trichlorobenzene (TCB) and γ-hexachlorocyclohexane (γHCH) by Phragmites australis were investigated under hydroponic conditions. The first step in sorption appears to be correlated with the hydrophobic nature of the compounds, since log-linear correlations were obtained between root concentration factor and partition coefficient (LogK{sub ow}). After 7 days of exposure, plant uptake of DCB, TCB, γHCH was significant with bioconcentration factors reaching 14, 19 and 15, respectively. Afterwards, uptake and translocation were seen to be more complex, with a loss of the simple relationship between uptake and LogK{sub ow}. Linear correlations between the bioconcentration/translocation factors and the physico-chemical properties of OCs were shown, demonstrating that translocation from roots to shoots increases with solubility and volatility of the OCs. This suggests that OC-translocation inside plants might result from the combination of two processes, xylem sap flow and vapor fluxes. {sup 14}C-phytovolatilization was measured and was correlated with the volatility of the compounds; the more volatile OCs being most the likely to be phytovolatilized from foliar surfaces (p = 0.0008). Thus, OC-uptake/translocation appears to proceed at a rate that depends mostly on the OCs hydrophobicity, solubility and volatility.

  8. Phragmites australis + Typha latifolia Community Enhanced the Enrichment of Nitrogen and Phosphorus in the Soil of Qin Lake Wetland

    Directory of Open Access Journals (Sweden)

    Zhiwei Ge

    2017-01-01

    Full Text Available Aquatic plants play an essential role and are effective in mitigating lake eutrophication by forming complex plant-soil system and retaining total nitrogen (TN and phosphorus (TP in soils to ultimately reduce their quantities in aquatic systems. Two main vegetation types (Phragmites australis community and P. australis + Typha latifolia community of Qin Lake wetland were sampled in this study for the analysis of TN and TP contents and reserves in the wetland soils. The results showed that (1 the consumption effect of Qin Lake wetland on soluble N was much more significant than on soluble P. (2 The efficiency of TN enrichment in wetland soil was enhanced by vegetation covering of P. australis and T. latifolia. (3 Wetland soil P was consumed by P. australis community and this pattern was relieved with the introduction of T. latifolia. (4 According to the grey relativity analysis, the most intensive interaction between plants and soil occurred in summer. In addition, the exchange of N in soil-vegetation system primarily occurred in the 0–15 cm soil layer. Our results indicated that vegetation covering was essential to the enrichment of TN and TP, referring to the biology-related fixation in the wetland soil.

  9. Determining the best phenological state for accurate mapping of Phragmites australis in wetlands using time series multispectral satellite data

    Science.gov (United States)

    Rupasinghe, P. A.; Markle, C. E.; Marcaccio, J. V.; Chow-Fraser, P.

    2017-12-01

    Phragmites australis (European common reed), is a relatively recent invader of wetlands and beaches in Ontario. It can establish large homogenous stands within wetlands and disperse widely throughout the landscape by wind and vehicular traffic. A first step in managing this invasive species includes accurate mapping and quantification of its distribution. This is challenging because Phragimtes is distributed in a large spatial extent, which makes the mapping more costly and time consuming. Here, we used freely available multispectral satellite images taken monthly (cloud free images as available) for the calendar year to determine the optimum phenological state of Phragmites that would allow it to be accurately identified using remote sensing data. We analyzed time series, Landsat-8 OLI and Sentinel-2 images for Big Creek Wildlife Area, ON using image classification (Support Vector Machines), Normalized Difference Vegetation Index (NDVI) and Normalized Difference Water Index (NDWI). We used field sampling data and high resolution image collected using Unmanned Aerial Vehicle (UAV; 8 cm spatial resolution) as training data and for the validation of the classified images. The accuracy for all land cover classes and for Phragmites alone were low at both the start and end of the calendar year, but reached overall accuracy >85% by mid to late summer. The highest classification accuracies for Landsat-8 OLI were associated with late July and early August imagery. We observed similar trends using the Sentinel-2 images, with higher overall accuracy for all land cover classes and for Phragmites alone from late July to late September. During this period, we found the greatest difference between Phragmites and Typha, commonly confused classes, with respect to near-infrared and shortwave infrared reflectance. Therefore, the unique spectral signature of Phragmites can be attributed to both the level of greenness and factors related to water content in the leaves during late

  10. Early successional stages of reed Phragmites australis vegetations and its importance for the Bearded Reedling Panurus biarmicus in Oostvaardersplassen, The Netherlands

    NARCIS (Netherlands)

    Beemster, Nico; Troost, Els; Platteeuw, Maarten

    2010-01-01

    A study on Bearded Reed ling Panurus biarmicus feeding habits in combination with a sample-wise breeding bird survey of the marshland zones of the Dutch wetland Oostvaardersplassen shows clear-cut spatial differences in densities and habitat use. The more mature stands of Reed Phragmites australis

  11. Changes in soluble metal concentrations induced by variable water table levels as response to liming and Phragmites australis growth in metal-polluted wetland soils: Management effectiveness

    NARCIS (Netherlands)

    Gonzalez Alcaraz, M.N.; van Gestel, C.A.M.

    2016-01-01

    This study aimed to assess the effectiveness of liming and Phragmites australis growth for the management of metal-polluted wetland soils under fluctuating water table levels. Soil columns (20 cm in diameter and 60 cm high) were constructed with two soil types (pH ~ 6.4 and pH ~ 3.1) and four

  12. Die-back of Phragmites australis in European wetlands: an overview of the European Research Programme on Reed Die-Back and Progression (1993-1994)

    NARCIS (Netherlands)

    Van der Putten, W.H.

    1997-01-01

    Reed (Phragmites australis (Cav.) Trin. ex Steudel) is one of the dominant plant species in European land-water ecotones. During the past decades reed belts have died back, especially in central and eastern Europe. The aim of the European Research Programme on Reed Die-back and Progression (EUREED),

  13. Removal of Cu, Zn, Pb, and Cr from Yangtze Estuary Using the Phragmites australis Artificial Floating Wetlands

    Directory of Open Access Journals (Sweden)

    Xiaofeng Huang

    2017-01-01

    Full Text Available Contamination of heavy metals would threaten the water and soil resources; phytoremediation can be potentially used to remediate metal contaminated sites. We constructed the Phragmites australis artificial floating wetlands outside the Qingcaosha Reservoir in the Yangtze Estuary. Water characteristic variables were measured in situ by using YSI Professional Pro Meter. Four heavy metals (copper, zinc, lead, and chromium in both water and plant tissues were determined. Four heavy metals in estuary water were as follows: 0.03 mg/Kg, 0.016 mg/Kg, 0.0015 mg/Kg, and 0.004 mg/Kg. These heavy metals were largely retained in the belowground tissues of P. australis. The bioaccumulation (BAF and translation factor (TF value of four heavy metals were affected by the salinity, temperature, and dissolved oxygen. The highest BAF of each metal calculated was as follows: Cr (0.091 in winter > Cu (0.054 in autumn > Pb (0.016 in summer > Zn (0.011 in summer. Highest root-rhizome TF values were recorded for four metals: 6.450 for Cu in autumn, 2.895 for Zn in summer, 7.031 for Pb in autumn, and 2.012 for Cr in autumn. This indicates that the P. australis AFW has potential to be used to protect the water of Qingcaosha Reservoir from heavy metal contamination.

  14. Monitoring Phragmites australis increases from 1937 to 1976 in the Siyai Lagoon (Natal, South Africa by means of air photo interpretation

    Directory of Open Access Journals (Sweden)

    P. J. Weisser

    1981-11-01

    Full Text Available The colonization o f the Siyai Lagoon on the north coast of Natal by Phragmites australis was studied by means of  air photo interpretation. It was possible to locate and estimate P. australis areas for 1957 (0,74 ha, 1965 (1,65 ha, 1969 (1,93 ha and 1976 (2,94 ha. Phragmites australis first inhabited the shores of the middle section o f the lagoon followed by rapid expansion in the lower section. The upper section was colonized only at its lower end by expansion from the middle section. It is suggested that P. australis was unsuccessful in this section because of competition by the  Hibiscus tiliaceus—Barringtonia racemosa  Lagoon Fringe Forest. This same community is shading out  P. australis in some places. The notable increase in the rate of advance of land and littoral vegetation into the Siyai Lagoon was caused by sugar farming activities leading to erosion and sedimentation in the lagoon. A vegetation age gradient was observed from the upper section to the mouth region. The colonization of most of the Siyai Lagoon except the immediate mouth zone by  P. australis Reedswamp and  Hibiscus tiliaceus—Barringtonia racemosa Lagoon Fringe Forest, can be expected before the turn of the century. Dredging and mechanical control of vegetation will become necessary if major open water spaces are to be maintained.

  15. Water and Energy Balance in Response to the Removal of Invasive Phragmites Australis in a Riparian Wetland

    Science.gov (United States)

    Mykleby, P.; Lenters, J. D.; Cutrell, G. J.; Herrman, K.; Istanbulluoglu, E.; Scott, D.

    2011-12-01

    Vegetation plays an important role in the surface energy and water balance of wetlands. Transpiration from phreatophytes, in particular, withdraws water directly from groundwater, often impacting streamflow rates in adjacent tributaries. In the Republican River basin of the Central Plains (USA), streamflow has declined significantly in the past 30-40 years. Invasive vegetation species (such as Phragmites australis) have been removed from portions of the riparian corridor in an effort to halt or reverse the downward trend in streamflow. In this study, we investigated the energy and water balance of a P. australis-dominated riparian wetland in south-central Nebraska to assess the potential effectiveness of such an approach. Evapotranspiration (ET) rates were measured during two growing seasons - one being 2009, when the P. australis was at full growth, and the other during 2010, after the vegetation had been sprayed with herbicide (and remained only as dead, standing biomass). Energy balance measurements at the field site included net radiation, heat storage rates in the canopy, soil, and standing water, and sensible heat flux, which was measured using a large-aperture scintillometer (LAS). Latent heat flux (i.e., ET) was calculated as a residual of the energy balance, and comparisons were made between the two growing seasons. As a result of the spraying of the P. australis vegetation, season-mean ET rates dropped from 4.4 mm day-1 in 2009 to 3.0 mm day-1 in 2010. This decrease in ET was associated with a large increase in sensible heat flux, which more than doubled between the two years (from 33 W m-2 in 2009 to 76 W m-2 in 2010). Meteorological conditions at the site were slightly different from one year to the next, but the differences were not large enough to account for the dramatic changes in latent and sensible heat flux that were observed. We conclude, therefore, that the majority of the ~30% decrease in ET (and ~130% increase in sensible heat flux) was the

  16. Identification, Development, and Release of Insect Biocontrol Agents for the Management of Phragmites australis

    Science.gov (United States)

    2014-07-01

    currently testing the pathogenicity of different isolates on P. australis germination and results are extremely diverse. Isolates and P. australis...pathogenicity. While these Pythium species have large effects on seed germination , it is doubtful that they can contribute to reductions in P...potential agents for in-depth study; (2) outline and report initial testing procedures and results of host-specificity studies of identified agents; (3

  17. Uptake and Bioaccumulation of Pentachlorophenol by Emergent Wetland Plant Phragmites australis (Common Reed) in Cadmium Co-contaminated Soil.

    Science.gov (United States)

    Hechmi, Nejla; Ben Aissa, Nadhira; Abdenaceur, Hassen; Jedidi, Naceur

    2015-01-01

    Despite many studies on phytoremediation of soils contaminated with either heavy metals or organics, little information is available on the effectiveness of phytoremediation of co-occurring metal and organic pollutants especially by using wetland species. Phragmites australis is a common wetland plant and its potential for phytoremediation of cadmium pentachlorophenol (Cd-PCP) co-contaminated soil was investigated. A greenhouse study was executed to elucidate the effects of Cd (0, 10, and 20 mg kg(-1)) without or with PCP (0, 50, and 250 mg kg(-1)) on the growth of the wetland plant P. australis and its uptake, accumulation and removal of pollutant from soils. After 75 days, plant biomass was significantly influenced by interaction of Cd and PCP and the effect of Cd on plant growth being stronger than that of PCP. Coexistence of PCP at low level lessened Cd toxicity to plants, resulting in improved plant growth and increased Cd accumulation in plant tissues. The dissipation of PCP in soils was significantly influenced by interactions of Cd, PCP and plant presence or absence. As an evaluation of soil biological activities after remediation soil enzyme was measured.

  18. Akkumulation von L-Malat und D-Lactat in Arabidopsis thaliana und Laccase/HBT-vermittelte Delignifizierung von Spartina alterniflora und Phragmites australis

    OpenAIRE

    Heil, Alexander

    2016-01-01

    The current work contains two projects "Accumulation of L-malate and D-lactate in Arabidopsis thaliana" (A) "Laccase/HBT mediated delignification of Spartina alterniflora and Phragmites australis" (B). In project A, L-malate and D-lactate accumulated in A. thaliana plants. The accumulation of L-malate is carried out by modification of the plant metabolism with the enzymes PEPC, MDH and the tonoplast dicarboxylate transporter (TDT). Gene pepci2 (Hydrilla verticillata), mdh5 (Zea mays) and tdt ...

  19. Phragmites australis management in the United States: 40 years of methods and outcomes

    OpenAIRE

    Hazelton, Eric L. G.; Mozdzer, Thomas J.; Burdick, David M.; Kettenring, Karin M.; Whigham, Dennis F.

    2014-01-01

    Studies on invasive plant management are often short in duration and limited in the methods tested, and lack an adequate description of plant communities that replace the invader following removal. Here we present a comprehensive review of management studies on a single species, in an effort to elucidate future directions for research in invasive plant management. We reviewed the literature on Phragmites management in North America in an effort to synthesize our understanding of management ef...

  20. Assessing leaf spectral properties of Phragmites australis impacted by acid mine drainage

    CSIR Research Space (South Africa)

    Van Deventer, Heidi

    2014-07-01

    Full Text Available decrease in the NIR24, and similarly the radionuclides Cs and Sr at Chernobyl were highly negatively correlated to the REP, green and NIR regions26. An opposite trend was observed in plants exposed to Cd24, Pb27, a combination of heavy metals (As, Cd, Cr.... Davids C, Tyler AN. Detecting contamination-induced tree stress within the Chernobyl exclusion zone. Remote Sens Environ. 2003;85(1):30–38. http:// dx.doi.org/10.1016/S0034-4257(02)00184-0 Research Article Assessing leaf spectral properties of Phragmites...

  1. Cosmopolitan species as ecophysiological models for responses to global change: the common reed Phragmites australis

    Czech Academy of Sciences Publication Activity Database

    Eller, F.; Skálová, Hana; Caplan, J. S.; Bhattarai, G. P.; Burger, M. K.; Cronin, J. T.; Guo, Wen-Yong; Guo, X.; Hazelton, E. L. G.; Kettenring, K. M.; Lambertini, C.; McCormick, M. K.; Meyerson, L. A.; Mozdzer, T. J.; Pyšek, Petr; Sorrell, B. K.; Whigham, D. F.; Brixi, H.

    2017-01-01

    Roč. 8, NOV 16 (2017), s. 1-24, č. článku 1833. ISSN 1664-462X R&D Projects: GA ČR GB14-36079G; GA ČR(CZ) GA14-15414S Grant - others:AV ČR(CZ) AP1002 Program:Akademická prémie - Praemium Academiae Institutional support: RVO:67985939 Keywords : Phragmites * ecophysiology * global change Subject RIV: EH - Ecology, Behaviour OBOR OECD: Ecology Impact factor: 4.298, year: 2016

  2. Multiple antibiotic resistance patterns of rhizospheric bacteria isolated from Phragmites australis growing in constructed wetland for distillery effluent treatment.

    Science.gov (United States)

    Chaturvedi, Sonal; Chandra, Ram; Rai, Vibhuti

    2008-01-01

    Susceptibility patterns of 12 different antibiotics were investigated against rhizospheric bacteria isolated from Phragmites australis from three different zones i.e. upper (0-5 cm), middle (5-10 cm), lower (10-15 cm) in constructed wetland system with and without distillery effluent. The major pollutants of distillery effluent were phenols, sulphide, heavy metals, and higher levels of biological oxygen demand (BOD), chemical oxygen demand (COD) etc. The antibiotic resistance properties of bacteria were correlated with the heavy metal tolerance (one of distillery pollutant). Twenty-two species from contaminated and seventeen species from non-contaminated site were tested by agar disc-diffusion method. The results revealed that more than 63% of total isolates were resistance towards one or more antibiotics tested from all the three different zones of contaminated sites. The multiple-drug resistance property was shown by total 8 isolates from effluent contaminated region out of which 3 isolates were from upper zone, 3 isolates from middle zone and 2 isolates were from lower zone. Results indicated that isolates from contaminated rhizosphere were found more resistant to antibiotics than isolates from non-contaminated rhizosphere. Further this study produces evidence suggesting that tolerance to antibiotics was acquired by isolates for the adaptation and detoxification of all the pollutants present in the effluent at contaminated site. This consequently facilitated the phytoremediation of effluent, which emerges the tolerance and increases resistance to antibiotics.

  3. Adaptive response of arbuscular mycorrhizal symbiosis to accumulation of elements and translocation in Phragmites australis affected by cadmium stress.

    Science.gov (United States)

    Huang, Xiaochen; Ho, Shih-Hsin; Zhu, Shishu; Ma, Fang; Wu, Jieting; Yang, Jixian; Wang, Li

    2017-07-15

    Arbuscular mycorrhizal (AM) fungi have been reported to play a central role in improving plant tolerance to cadmium (Cd)-contaminated sites. This is achieved by enhancing both the growth of host plants and the nutritive elements in plants. This study assessed potential regulatory effects of AM symbiosis with regard to nutrient uptake and transport, and revealed different response strategies to various Cd concentrations. Phragmites australis was inoculated with Rhizophagus irregularis in the greenhouse cultivation system, where it was treated with 0-20 mg L -1 of Cd for 21days to investigate growth parameters, as well as Cd and nutritive element distribution in response to AM fungus inoculation. Mycorrhizal plants showed a higher tolerance, particularly under high Cd-level stress in the substrate. Moreover, our results determined the roots as dominant Cd reservoirs in plants. The AM fungus improved Cd accumulation and saturated concentration in the roots, thus inhibiting Cd uptake to shoots. The observed distributions of nutritive elements and the interactions among these indicated the highest microelement contribution to roots, Ca contributed maximally in leaves, and K and P contributed similarly under Cd stress. In addition, AM fungus inoculation effectively impacted Mn and P uptake and accumulation while coping with Cd toxicity. This study also demonstrated translocation factor from metal concentration (TF) could be a good parameter to evaluate different transportation strategies induced by various Cd stresses in contrast to the bioconcentration factor (BCF) and translocation factor from metal accumulation (TF'). Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. Biogas properties and enzymatic analysis during anaerobic fermentation of Phragmites australis straw and cow dung: influence of nickel chloride supplement.

    Science.gov (United States)

    Tian, Yonglan; Zhang, Huayong; Chai, Yang; Wang, Lijun; Mi, Xueyue; Zhang, Luyi; Ware, Maxwell Adam

    2017-02-01

    The importance of nickel (added as NiCl 2 ) on mesophilic anaerobic fermentation of Phragmites australis straw and cow dung was demonstrated by investigating the biogas properties, pH values, organic matter degradation [chemical oxygen demand (COD)] and enzyme activities (cellulase, protease and dehydrogenase) during the fermentation process. The results showed that Ni 2+ addition increased the cumulative biogas yields by >18 % by improving the efficiency of first peak stage and bringing forward the second peak stage. The pH values were not significantly influenced by Ni 2+ addition (p > 0.05). Biogas yields were associated with variations in COD concentrations rather than momentary concentrations. At the start-up stage of fermentation (4th day), the biogas yields increased gradually together with the increase of dehydrogenase activities at elevated Ni 2+ concentrations when cellulase and protease activities were similar in all test groups. It is suggested that Ni 2+ addition was mainly dependent on the methanogenic stage. After the start-up stage, the impact of Ni 2+ addition on biogas production was mainly dependent on its effect on cellulase activities, rather than protease or dehydrogenase activities.

  5. Growth of Phragmites australis (Cav.) Trin ex. Steudel in mine water treatment wetlands: effects of metal and nutrient uptake

    International Nuclear Information System (INIS)

    Batty, Lesley C.; Younger, Paul L.

    2004-01-01

    The abandoned mine of Shilbottle Colliery, Northumberland, UK is an example of acidic spoil heap discharge that contains elevated levels of many metals. Aerobic wetlands planted with the common reed, Phragmites australis, were constructed at the site to treat surface runoff from the spoil heap. The presence of a perched water table within the spoil heap resulted in the lower wetlands receiving acidic metal contaminated water from within the spoil heap while the upper wetland receives alkaline, uncontaminated surface runoff from the revegetated spoil. This unique situation enabled the comparison of metal uptake and growth of plants used in treatment schemes in two cognate wetlands. Results indicated a significant difference in plant growth between the two wetlands in terms of shoot height and seed production. Analyses of metal and nutrient concentrations within plant tissues provided the basis for three hypotheses to explain these differences: (i) the toxic effects of high levels of metals in shoot tissues (ii) the inhibition of Ca (an essential nutrient) uptake by the presence of metals and H + ions, and (iii) low concentrations of bioavailable nitrogen sources resulting in nitrogen deficiency. This has important implications for the engineering of constructed wetlands in terms of the potential success of plant establishment and vegetation development

  6. Root-secreted allelochemical in the noxious weed Phragmites australis deploys a reactive oxygen species response and microtubule assembly disruption to execute rhizotoxicity.

    Science.gov (United States)

    Rudrappa, Thimmaraju; Bonsall, Justin; Gallagher, John L; Seliskar, Denise M; Bais, Harsh P

    2007-10-01

    Phragmites australis is considered the most invasive plant in marsh and wetland communities in the eastern United States. Although allelopathy has been considered as a possible displacing mechanism in P. australis, there has been minimal success in characterizing the responsible allelochemical. We tested the occurrence of root-derived allelopathy in the invasiveness of P. australis. To this end, root exudates of two P. australis genotypes, BB (native) and P38 (an exotic) were tested for phytotoxicity on different plant species. The treatment of the susceptible plants with P. australis root exudates resulted in acute rhizotoxicity. It is interesting to note that the root exudates of P38 were more effective in causing root death in susceptible plants compared to the native BB exudates. The active ingredient in the P. australis exudates was identified as 3,4,5-trihydroxybenzoic acid (gallic acid). We tested the phytotoxic efficacy of gallic acid on various plant systems, including the model plant Arabidopsis thaliana. Most tested plants succumbed to the gallic acid treatment with the exception of P. australis itself. Mechanistically, gallic acid treatment generated elevated levels of reactive oxygen species (ROS) in the treated plant roots. Furthermore, the triggered ROS mediated the disruption of the root architecture of the susceptible plants by damaging the microtubule assembly. The study also highlights the persistence of the exuded gallic acid in P. australis's rhizosphere and its inhibitory effects against A. thaliana in the soil. In addition, gallic acid demonstrated an inhibitory effect on Spartina alterniflora, one of the salt marsh species it successfully invades.

  7. Contamination of Soil with Pb and Sb at a Lead-Acid Battery Dumpsite and Their Potential Early Uptake by Phragmites australis

    Directory of Open Access Journals (Sweden)

    Abraham Jera

    2017-01-01

    Full Text Available Recycling of spent Lead-Acid Batteries (LABs and disposal of process slag potentially contaminate soil with Pb and Sb. Total and available concentrations of Pb and Sb in three soil treatments and parts of Phragmites australis were determined by atomic absorption spectrophotometry. Soil with nonrecycled slag (NR had higher total metal concentrations than that with recycled slag (RS. Low available fractions of Pb and Sb were found in the soil treatments before planting P. australis. After 16 weeks of growth of P. australis, the available fractions of Pb had no statistical difference from initial values (p>0.05 while available Sb fractions were significantly lower when compared with their initial values (p<0.05. Metal transfer factors showed that P. australis poorly accumulate Pb and Sb in roots and very poorly translocate them to leaves after growing for 8 and 16 weeks. It may be a poor phytoextractor of Pb and Sb in metal-contaminated soil at least for the 16 weeks of its initial growth. However, the plant established itself on the metalliferous site where all vegetation had been destroyed. This could be useful for potential ecological restoration. The long-term phytoextraction potential of P. australis in such environments as LABs may need further investigation.

  8. Accumulation of nutrients and heavy metals in Phragmites australis (Cav.) Trin. ex Steudel and Bolboschoenus maritimus (L.) Palla in a constructed wetland of the Venice lagoon watershed

    International Nuclear Information System (INIS)

    Bragato, Claudia; Brix, Hans; Malagoli, Mario

    2006-01-01

    A recently constructed wetland, located in the Venice lagoon watershed, was monitored to investigate growth dynamics, nutrient and heavy metal shoot accumulation of the two dominating macrophytes: Phragmites australis and Bolboschoenus maritimus. Investigations were conducted over a vegetative season at three locations with different distance to the inlet point to assess effects on vegetation. The distance from the inlet did not affect either shoot biomass or nutrients (N, P, K and Na) and heavy metals (Cr, Ni, Cu and Zn) shoot content. With the exception of Na, nutrient and heavy metal concentrations were higher in shoots of P. australis than in B. maritimus. Heavy metal concentration in the incoming water and in the soil was not correlated to the plant content of either species. Shoot heavy metal concentrations were similar to those reported in the current literature, but accumulation generally increased towards the end of the growing season. - Heavy metal shoot concentration in Phragmites australis and Bolboschoenus maritimus increased significantly at the end of the growing season

  9. Composting Phragmites australis Cav. plant material and compost effects on soil and tomato (Lycopersicon esculentum Mill.) growth.

    Science.gov (United States)

    Toumpeli, Anna; Pavlatou-Ve, Athina K; Kostopoulou, Sofia K; Mamolos, Andreas P; Siomos, Anastasios S; Kalburtji, Kiriaki L

    2013-10-15

    Composting organic residues is a friendly to the environment alternative to producing fertilizer. This research was carried out to study the process of composting Phragmites australis Cav. plant material alone or with animal manure on a pilot-scale, to evaluate firstly the quality of the composts produced and secondly, using a pot experiment, the effects of their application on soil physicochemical characteristics and tomato plants development. For the compost production a randomized complete block design was used with five treatments (five compost types) and four replications. For the pot experiment, a completely randomized design was used with 17 treatments (plain soil, soil with synthetic fertilizer and the application of five compost types, at three rates each) and five replications. Compost N increased with composting time, while C/N ratio decreased significantly and by the end it ranged from 43.3 for CM to 22.6 for CY. Compost pH became almost neutral, ranging from 6.73 for CY to 7.21 for CM3Y3AM4 by the end. Compost combinations CY7AM3 and CM7AM3 had a more positive influence on the soil physicochemical characteristics than the others. Soil N, P, Ca and Mg concentrations and the reduction of clay dispersion were the highest when CM7AM3 compost was added. The macro-aggregate stability was the highest for CY7AM3, which also sustained plant growth. The latter compost combination improved most of the soil physicochemical characteristics and plant growth especially, when the application rate was 4% (w/w), which equals to 156 Mg ha(-1). Copyright © 2013 Elsevier Ltd. All rights reserved.

  10. Effects of sediment burial disturbance on macro and microelement dynamics in decomposing litter of Phragmites australis in the coastal marsh of the Yellow River estuary, China.

    Science.gov (United States)

    Sun, Zhigao; Mou, Xiaojie

    2016-03-01

    From April 2008 to November 2009, a field decomposition experiment was conducted to investigate the effects of sediment burial on macro (C, N) and microelement (Pb, Cr, Cu, Zn, Ni, and Mn) variations in decomposing litter of Phragmites australis in the coastal marsh of the Yellow River estuary. Three one-off sediment burial treatments [no sediment burial (0 mm year(-1), S0), current sediment burial (100 mm year(-1), S10), and strong sediment burial (200 mm year(-1), S20)] were laid in different decomposition sites. Results showed that sediment burials showed significant influence on the decomposition rate of P. australis, in the order of S10 (0.001990 day(-1)) ≈ S20 (0.001710 day(-1)) > S0 (0.000768 day(-1)) (p macro and microelement in decomposing litters of the three burial depths exhibited different temporal variations except for Cu, Zn, and Ni. No significant differences in C, N, Pb, Cr, Zn, and Mn concentrations were observed among the three burial treatments except for Cu and Ni (p > 0.05). With increasing burial depth, N, Cr, Cu, Ni, and Mn concentrations generally increased, while C, Pb, and Zn concentrations varied insignificantly. Sediment burial was favorable for C and N release from P. australis, and, with increasing burial depth, the C release from litter significantly increased, and the N in litter shifted from accumulation to release. With a few exceptions, Pb, Cr, Zn, and Mn stocks in P. australis in the three treatments evidenced the export of metals from litter to environment, and, with increasing burial depth, the export amounts increased greatly. Stocks of Cu and Ni in P. australis in the S10 and S20 treatments were generally positive, evidencing incorporation of the two metals in most sampling times. Except for Ni, the variations of C, N, Pb, Cr, Cu, Zn, and Mn stocks in P. australis in the S10 and S20 treatments were approximated, indicating that the strong burial episodes (S20) occurred in P. australis marsh in the future

  11. Effects of arbuscular mycorrhizal fungi and soil nutrient addition on the growth of Phragmites australis under different drying-rewetting cycles.

    Science.gov (United States)

    Liang, Jin-Feng; An, Jing; Gao, Jun-Qin; Zhang, Xiao-Ya; Yu, Fei-Hai

    2018-01-01

    The frequency of soil drying-rewetting cycles is predicted to increase under future global climate change, and arbuscular mycorrhizal fungi (AMF) are symbiotic with most plants. However, it remains unknown how AMF affect plant growth under different frequencies of soil drying-rewetting cycles. We subjected a clonal wetland plant Phragmites australis to three frequencies of drying-rewetting cycles (1, 2, or 4 cycles), two nutrient treatments (with or without), and two AMF treatments (with or without) for 64 days. AMF promoted the growth of P. australis, especially in the 2 cycles of the drying-rewetting treatment. AMF had a significant positive effect on leaf mass and number of ramets in the 2 cycles of the drying-rewetting treatment with nutrient addition. In the 2 cycles of drying-rewetting treatment without nutrient addition, AMF increased leaf area and decreased belowground to aboveground biomass ratio. These results indicate that AMF may assist P. australis in coping with medium frequency of drying-rewetting cycles, and provide theoretical guidance for predicting how wetland plants respond to future global climate change.

  12. [Effects of water table manipulation on leaf photosynthesis, morphology and growth of Phragmites australis and Imperata cylindrica in the reclaimed tidal wetland at Dongtan of Chongming Island, China].

    Science.gov (United States)

    Zhong, Qi-Cheng; Wang, Jiang-Tao; Zhou, Jian-Hong; Ou, Qiang; Wang, Kai-Yun

    2014-02-01

    During the growing season of 2011, the leaf photosynthesis, morphological and growth traits of Phragmites australis and Imperata cylindrica were investigated along a gradient of water table (low, medium and high) in the reclaimed tidal wetland at the Dongtan of Chongming Island in the Yangtze Estuary of China. A series of soil factors, i. e., soil temperature, moisture, salinity and inorganic nitrogen content, were also measured. During the peak growing season, leaf photosynthetic capacity of P. australis in the wetland with high water table was significantly lower than those in the wetland with low and medium water tables, and no difference was observed in leaf photosynthetic capacity of I. cylindrica at the three water tables. During the entire growing season, at the shoot level, the morphological and growth traits of P. australis got the optimum in the wetland with medium water table, but most of the morphological and growth traits of I. cylindrica had no significant differences at the three water tables. At the population level, the shoot density, leaf area index and aboveground biomass per unit area were the highest in the wetland with high water table for P. australis, but all of the three traits were the highest in the wetland with low water table for I. cylindrica. At the early growing season, the rhizome biomass of P. australis in the 0-20 cm soil layer had no difference at the three water tables, and the rhizome biomass of I. cylindrica in the 0-20 cm soil layer in the wetland with high water table was significantly lower than those in the wetland with low and medium water table. As a native hygrophyte before the reclamation, the variations of performances of P. australis at the three water tables were probably attributed to the differences in the soil factors as well as the intensity of competition from I. cylindrica. To appropriately manipulate water table in the reclaimed tidal wetland may restrict the growth and propagation of the mesophyte I

  13. Genetic and epigenetic diversity and structure of Phragmites australis from local habitats of the Songnen Prairie using amplified fragment length polymorphism markers.

    Science.gov (United States)

    Qiu, T; Jiang, L L; Yang, Y F

    2016-08-19

    The genetic and epigenetic diversity and structure of naturally occurring Phragmites australis populations occupying two different habitats on a small spatial scale in the Songnen Prairie in northeastern China were investigated by assessing amplified fragment length polymorphisms (AFLPs) and methylation-sensitive amplified polymorphisms (MSAPs) through fluorescent capillary detection. The two groups of P. australis were located in a seasonal waterlogged low-lying and alkalized meadow with a pH of 8-8.5 and in an alkaline patch without accumulated rainwater and with a pH greater than 10. These groups showed high levels of genetic diversity at the habitat level based on the percentage of polymorphic bands (90.32, 82.56%), Nei's gene diversity index (0.262, 0.248), and the Shannon diversity index (0.407, 0.383). Although little is known about the between-habitat genetic differentiation of P. australis on a small spatial scale, our results implied significant genetic differentiation between habitats. Extensive epigenetic diversity within habitats, along with clear differentiation, was found. Specifically, the former habitat (Habitat 1, designated H1) harbored higher levels of genetic and epigenetic diversity than the latter (Habitat 2, designated H2), and population-level diversity was also high. This study represents one of few attempts to predict habitat-based genetic differentiation of reeds on a small scale. These assessments of genetic and epigenetic variation are integral aspects of molecular ecological studies on P. australis. Possible causes for within- and between-habitat genetic and epigenetic variations are discussed.

  14. Invasion of Old World Phragmites australis in the New World: precipitation and temperature patterns combined with human influences redesign the invasive niche.

    Science.gov (United States)

    Guo, Wen-Yong; Lambertini, Carla; Li, Xiu-Zhen; Meyerson, Laura A; Brix, Hans

    2013-11-01

    After its introduction into North America, Euro-Asian Phragmites australis became an aggressive invasive wetland grass along the Atlantic coast of North America. Its distribution range has since expanded to the middle, south and southwest of North America, where invasive P. australis has replaced millions of hectares of native plants in inland and tidal wetlands. Another P. australis invasion from the Mediterranean region is simultaneously occurring in the Gulf region of the United States and some countries in South America. Here, we analysed the occurrence records of the two Old World invasive lineages of P. australis (Haplotype M and Med) in both their native and introduced ranges using environmental niche models (ENMs) to assess (i) whether a niche shift accompanied the invasions in the New World; (ii) the role of biologically relevant climatic variables and human influence in the process of invasion; and (iii) the current potential distribution of these two lineages. We detected local niche shifts along the East Coast of North America and the Gulf Coast of the United States for Haplotype M and around the Mississippi Delta and Florida of the United States for Med. The new niche of the introduced Haplotype M accounts for temperature fluctuations and increased precipitation. The introduced Med lineage has enlarged its original subtropical niche to the tropics-subtropics, invading regions with a high annual mean temperature (> ca. 10 °C) and high precipitation in the driest period. Human influence is an important factor for both niches. We suggest that an increase in precipitation in the 20th century, global warming and human-made habitats have shaped the invasive niches of the two lineages in the New World. However, as the invasions are ongoing and human and natural disturbances occur concomitantly, the future distribution ranges of the two lineages may diverge from the potential distribution ranges detected in this study. © 2013 John Wiley & Sons Ltd.

  15. Osmotic and ionic effects of NaCl and Na2SO4 salinity on Phragmites australis

    DEFF Research Database (Denmark)

    Pagter, Majken; Bragato, Claudia; Malagoli, Mario

    2009-01-01

    Osmotic and ion-specific effects of NaCl and Na2SO4 on Phragmites australis (Cav.) Trin ex. Steud. were investigated in a laboratory experiment by examining effects of iso-osmotic solutions of NaCl and Na2SO4 on growth, osmolality of cell sap, proline content, elemental composition and gas exchange....... Plants were supplied with a control standard nutrient solution (Ψ = -0.09 MPa) or solutions of NaCl or Na2SO4 at water potentials of -0.50, -1.09 or -1.74 MPa. Salt treatments increased root concentrations of Na and S or Cl, whereas P. australis had efficient mechanisms for exclusion of Na and S...... and partly Cl ions from the leaves. Incomplete exclusion of Cl from the leaves may affect aboveground biomass production, which was significantly more reduced by NaCl than Na2SO4. Stomatal conductance was negatively influenced by decreasing water potentials caused by NaCl or Na2SO4, implying that a non...

  16. Evaluación de la Remoción de Nitrógeno, Fósforo y Sulfuros en Agua Residual Doméstica, Utilizando Phragmites australis en Bioreactores

    OpenAIRE

    Correa-Torres, Sandra N; Gamarra, Yolanda; Salazar, Andrés A; Pitta, Natalia M

    2015-01-01

    En el presente estudio se evaluó la remoción de nitrógeno, fósforo y sulfuros en un agua residual doméstica mediante fitorremediación utilizando Phragmites australis en bioreactores a escala de laboratorio. Se determinó el estudio las condiciones hidráulicas en reactores analizando las variables de relación geométrica largo:ancho (4:1 y 1:4) y usando un medio filtrante (grava/arena). Adicionalmente se evaluó el crecimiento de P. australis en los reactores y se analizó los porcentajes de remoc...

  17. Fungal endophytes from seeds of invasive, non-native Phragmites australis and their potential role in germination and seedling growth

    Science.gov (United States)

    Shearin, Zackery R. C.; Filipek, Matthew; Desai, Rushvi; Bickford, Wesley A.; Kowalski, Kurt P.; Clay, Keith

    2018-01-01

    Background and aimsWe characterized fungal endophytes of seeds of invasive, non-native Phragmites from three sites in the Great Lakes region to determine if fungal symbiosis could contribute to invasiveness through their effects on seed germination and seedling growth.MethodsField-collected seeds were surface sterilized and plated on agar to culture endophytes for ITS sequencing. Prevalence of specific endophytes from germinated and non-germinated seeds, and from seedlings, was compared.ResultsOne-third of 740 seeds yielded endophyte isolates. Fifteen taxa were identified with Alternaria sp. representing 54% of all isolates followed by Phoma sp. (21%) and Penicillium corylophilum (12%). Overall germination of seeds producing an isolate (36%) was significantly higher than seeds not producing an isolate (20%). Penicillium in particular was strongly associated with increased germination of seeds from one site. Sixty-three isolates and 11 taxa were also obtained from 30 seedlings where Phoma, Penicillium and Alternaria respectively were most prevalent. There was a significant effect of isolating an endophyte from the seed on seedling growth.ConclusionsThese results suggest that many endophyte taxa are transmitted in seeds and can increase seed germination and seedling growth of invasive Phragmites. The role of fungal endophytes in host establishment, growth and invasiveness in nature requires further research.

  18. Spatial genetic structure in natural populations of Phragmites australis in a mosaic of saline habitats in the Yellow River Delta, China.

    Directory of Open Access Journals (Sweden)

    Lexuan Gao

    Full Text Available Determination of spatial genetic structure (SGS in natural populations is important for both theoretical aspects of evolutionary genetics and their application in species conservation and ecological restoration. In this study, we examined genetic diversity within and among the natural populations of a cosmopolitan grass Phragmites australis (common reed in the Yellow River Delta (YRD, China, where a mosaic of habitat patches varying in soil salinity was detected. We demonstrated that, despite their close geographic proximity, the common reed populations in the YRD significantly diverged at six microsatellite loci, exhibiting a strong association of genetic variation with habitat heterogeneity. Genetic distances among populations were best explained as a function of environmental difference, rather than geographical distance. Although the level of genetic divergence among populations was relatively low (F'(ST =0.073, weak but significant genetic differentiation, as well as the concordance between ecological and genetic landscapes, suggests spatial structuring of genotypes in relation to patchy habitats. These findings not only provided insights into the population dynamics of common reed in changing environments, but also demonstrated the feasibility of using habitat patches in a mosaic landscape as test systems to identify appropriate genetic sources for ecological restoration.

  19. An investigation of the possibility of mercury phytoremediation from Bandar ImamChlor-alkali plants' wastewater using Phragmites australis

    International Nuclear Information System (INIS)

    Tayebi, L.; Hamidian, A.H.; Danehkar, A.; Poorbagher, H.

    2016-01-01

    The Petrochemical industry is the most important and most widely active industries in the country. Due to the variety and complexity of industrial products, it also produces a wild range of pollutants. Mercury waste disposal from Chlor-alkali units is one of the fundamental problems of this industry. Various studies have shown that Phytoremediation system for removal of mercury from aqueous solutions is very efficient and, in some cases up to 95% of mercury has been removed from the solution. The purpose of this study was to evaluate the ability of common reed (Phragmites australis) in the removal of mercury from the Chlor-alkali effluent in Bandar Imam Petrochemical. Plant samples Harvested from Shadegan wetland were cultured hydroponically in plastic aquariums. Effluent samples which were taken from Chlor-alkali plants were added to the culture medium. An aquarium containing wastewater, water and nutrients was considered as control. Mercury concentrations in water and plant at 1, 3, 5 and 7 days were measured by Varian Spectra 220 Atomic Absorption Spectroscopy. The results showed that Time has a direct effect on mercury up taking by common reed. The common Reed absorption average was 2657.25 ppm within 7 days, that shows a high capacity of mercury absorption from Chlor-alkali plant effluents. Also In the study period, 96.25% of mercury were removed from common reed aquarium effulgent water.

  20. Uptake of radionuclides by a common reed (Phragmites australis (Cav.) Trin. ex Steud.) grown in the vicinity of the former uranium mine at Zirovski vrh

    Energy Technology Data Exchange (ETDEWEB)

    Cerne, Marko, E-mail: marko.cerne@ijs.s [Jozef Stefan Institute, Jamova cesta 39, SI-1000 Ljubljana (Slovenia); Smodis, Borut, E-mail: borut.smodis@ijs.s [Jozef Stefan Institute, Jamova cesta 39, SI-1000 Ljubljana (Slovenia); Strok, Marko, E-mail: marko.strok@ijs.s [Jozef Stefan Institute, Jamova cesta 39, SI-1000 Ljubljana (Slovenia)

    2011-04-15

    From uranium mining areas, in particular, the radionuclides are usually discharged to the environment during the mining and milling process. At the former uranium mine Zirovski vrh, Slovenia, mine waste and mill tailings were deposited at the Jazbec site and the Borst site, respectively. Plants grown in soils contaminated with the seepage waters from tailings may represent radiological concern if radionuclides from the uranium decay chain are transferred into the food chain. Uranium is usually accumulated in the roots and translocated to the shoots in limited amounts. Uranium plant accumulators are usually plants from Brassicaceae and Poaceae families. A common reed (Phragmites australis (Cav.) Trin. ex Steud.), a tall perennial grass, growing in a wetland habitats, accumulates metals in the above-ground parts. It may be used for phytoremediation of uranium-contaminated soils, because of high biomass production and high metal-accumulation potential. Preliminary results of radionuclide contents measured in such plants, growing on the deposit tailings are presented. A common reed, that was grown on the Borst tailings pile accumulated 8.6 {+-} 8 mBq/g dry weight (d.w.) and 2.4 {+-} 2 mBq/g dry weight (d.w.) of {sup 238}U in leaves and stems, respectively. In the paper, activity concentrations of other nuclides, i.e. {sup 226}Ra, {sup 210}Pb and {sup 40}K are also shown and discussed.

  1. Persistent Reductions in the Bioavailability of PCBs at a Tidally Inundated Phragmites australis Marsh Amended with Activated Carbon.

    Science.gov (United States)

    Sanders, James P; Andrade, Natasha A; Menzie, Charles A; Amos, C Bennett; Gilmour, Cynthia C; Henry, Elizabeth A; Brown, Steven S; Ghosh, Upal

    2018-06-05

    In situ amendment of sediments with highly sorbent materials like activated carbon (AC) is an increasingly viable strategy to reduce the bioavailability of persistent, sediment-associated contaminants to benthic communities. Because in situ sediment remediation is an emerging strategy, much remains to be learned about the field conditions under which amendments can be effective, the resilience of amendment materials toward extreme weather conditions, and the optimal design of engineered applications. Here we report the results of a multi-year, pilot-scale field investigation designed to measure the persistence and efficacy of AC amendments to reduce the bioavailability of polychlorinated biphenyls (PCBs) in an intertidal Phragmites marsh. The amendments tested were granular AC (GAC), GAC with a layer of sand, and a pelletized fine AC. Key metrics presented include vertically-resolved black carbon concentrations in sediment and PCB concentrations in sediment, porewater, and several invertebrate species. The results demonstrate that all three amendments withstood Hurricane Sandy and remained in place for the duration of the study, successfully reducing porewater PCB concentrations by 34-97%. Reductions in invertebrate bioaccumulation were observed in all amendment scenarios, with pelletized fine AC producing the most pronounced effect. Our findings support the use of engineered AC amendments in intertidal marshes, and can be used to inform amendment design, delivery, and monitoring at other contaminated sediment sites. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  2. Large-scale management of common reed, Phragmites australis, for paper production: A case study from the Liaohe Delta, China

    DEFF Research Database (Denmark)

    Brix, Hans; Ye, Siyuan; Laws, Edward A.

    2014-01-01

    The largest Phragmites reed field in the world, with a historical area of approximately 1000 km2, is located in the Liaohe Delta in northeastern China. The Phragmites wetlands are extensively managed to maximize the production of reed biomass for the paper industry. Based on satellite remote sens...

  3. Fungal disease prevention in seedlings of rice (Oryza sativa) and other grasses by growth-promoting seed-associated endophytic bacteria from invasive Phragmites australis

    Science.gov (United States)

    Verma, Satish K.; Kingsley, Kathryn L.; Bergen, Marshall S.; Kowalski, Kurt P.; White, James F.

    2018-01-01

    Non-cultivated plants carry microbial endophytes that may be used to enhance development and disease resistance of crop species where growth-promoting and protective microbes may have been lost. During seedling establishment, seedlings may be infected by several fungal pathogens that are seed or soil borne. Several species of Fusarium, Pythium and other water moulds cause seed rots during germination. Fusariumblights of seedlings are also very common and significantly affect seedling development. In the present study we screened nine endophytic bacteria isolated from the seeds of invasive Phragmites australis by inoculating onto rice, Bermuda grass (Cynodon dactylon), or annual bluegrass (Poa annua) seeds to evaluate plant growth promotion and protection from disease caused by Fusarium oxysporum. We found that three bacteria belonging to genus Pseudomonas spp. (SLB4-P. fluorescens, SLB6-Pseudomonas sp. and SY1-Pseudomonassp.) promoted seedling development, including enhancement of root and shoot growth, and stimulation of root hair formation. These bacteria were also found to increase phosphate solubilization in in vitro experiments. Pseudomonas sp. (SY1) significantly protected grass seedlings from Fusarium infection. In co-culture experiments, strain SY1 strongly inhibited fungal pathogens with 85.71% growth inhibition of F. oxysporum, 86.33% growth inhibition of Curvularia sp. and 82.14% growth inhibition of Alternaria sp. Seedlings previously treated with bacteria were found much less infected by F. oxysporum in comparison to non-treated controls. On microscopic observation we found that bacteria appeared to degrade fungal mycelia actively. Metabolite products of strain SY1 in agar were also found to inhibit fungal growth on nutrient media. Pseudomonas sp. (SY1) was found to produce antifungal volatiles. Polymerase chain reaction (PCR) amplification using specific primers for pyrrolnitirin synthesis and HCN (hydrogen cyanide) production

  4. Use of biological indexes of the common reed (Phragmites australis) seed progeny in the environmental safety of radioactive contaminated water bodies

    Energy Technology Data Exchange (ETDEWEB)

    Yavnyuk, A. [National Aviation University, Kiev (Ukraine); Shevtsova, N.; Gudkov, D. [Institute of Hydrobiology of the National Academy of Sciences, Kiev (Ukraine)

    2014-07-01

    Environmental protection requires effective monitoring system of radionuclide contamination and radiobiological effects as well as development of their prevention and minimizing measures for humans and biota. There is a majority of conventional techniques for living organisms' habitat quality assessment. One of the most widespread, convenient and accessible ones, is the seed progeny analysis, for example of conifers, cereals and wild herbaceous plants. Availability of vitality, growth, mutability indexes and abnormalities of vascular plant germs for environment quality express assessment was discussed in numerous publications. However, this point is studied insufficiently concerning aquatic vascular plants, forming communities playing significant role in radionuclides distribution in contaminated water bodies. Common reed (Phragmites australis (Trin) Ex. Steud) is a widespread species mostly dominating in air-aquatic vascular plant communities of freshwater bodies; it is a first-order {sup 137}Cs and {sup 90}Sr accumulating species. To assess the common reed germs growth indexes availability, seeds were sampled in polygon water bodies of different radionuclide contamination levels and 0.7-22 mcGy h{sup -1} total absorbed dose range, within the Chernobyl Exclusion Zone. In water bodies with background level of radionuclide contamination, for comparison, total absorbed dose varied in range of 0.03-0.3 mcGy h{sup -1}. Series of seeds germination experiments was carried out in laboratory conditions. Complex of germs indexes was investigated, conditionally divided into three groups: (1) Vitality indexes. In course of experiment series, vitality was assessed via germinating energy, germinating ability indexes, germination period (first and last germ appearance) and survivability study; (2) Growth indexes. Root and leaf length, occurrence of plant groups with different vegetative organs length were determined for germs growth speed assessment; (3) Teratological

  5. Phenotypic and genotypic variation of Phragmites australis: Comparison of populations in two human-made lakes of different age and history

    Czech Academy of Sciences Publication Activity Database

    Čurn, V.; Kubátová, B.; Vávřová, P.; Křiváčková; Suchá, O.; Čížková, Hana

    2007-01-01

    Roč. 86, - (2007), s. 321-330 ISSN 0304-3770 R&D Projects: GA ČR(CZ) GA526/06/0276 Institutional research plan: CEZ:AV0Z60870520 Keywords : Phragmites * Phenotypic variation * Genotypic variation * Lake * Clone Subject RIV: EF - Botanics Impact factor: 1.497, year: 2007

  6. Invasion of Old World Phragmites australis in the New World: precipitation and temperature patterns combined with human influences redesign the invasive niche

    DEFF Research Database (Denmark)

    Guo, Wen-Yong; Lambertini, Carla; Li, Xiu-Zhen

    2013-01-01

    niches. We suggest that an increase in precipitation in the 20(th) century, global warming and human-made habitats have shaped the invasive niches of the two lineages in the New World. However, as the invasions are on-going and human and natural disturbances occur concomitantly, the future distribution....... australis (Haplotype M and Med) in both their native and introduced ranges using environmental niche models (ENMs) to assess (i) whether a niche shift accompanied the invasions in the New World; (ii) the role of biologically relevant climatic variables and human influence in the process of invasion...... for temperature fluctuations and increased precipitation. The introduced Med lineage has enlarged its original subtropical niche to the tropics-subtropics, invading regions with a high annual mean temperature (> c. 10 °C) and high precipitation in the driest period. Human influence is an important factor for both...

  7. Influencia de la estación y de las especies Phragmites australis y Schoenoplectus californicus en la eliminación de materia orgánica y nutrientes contenidos en aguas servidas durante la operación de puesta en marcha de humedales construidos de flujo horizontal subsuperficial

    Directory of Open Access Journals (Sweden)

    Karen Rojas

    2013-01-01

    Full Text Available Las plantas de tratamiento de aguas servidas basadas en humedales construidos de flujo horizontal subsuperficial (HSS, logran su etapa de madurez después de dos años de operación. La eficiencia de operación de los sistemas depende de la estacionalidad, tipo de plantas y periodo de madurez del sistema. El objetivo de este trabajo es evaluar la influencia de la estación y de las especies Phragmites australis y Schoenoplectus californicus, en la eficiencia de depuración en materia orgánica y nutrientes de unidades de HSS, durante la etapa operativa de puesta en marcha. Con esta finalidad, se implementaron cuatro unidades de HSS bajo condiciones climáticas mediterráneas australes. Dos unidades fueron plantadas con Phragmites australis y las otras dos, con Schoenoplectus californicus. En este trabajo se presentan los resultados de la etapa de puesta en marcha, que cubre los primeros 193 días de operación. Se evaluó la influencia de la especie de planta y la estación (invierno y primavera, en la eficiencia de depuración de materia orgánica (Demanda Biológica de Oxígeno, DBO5, y Demanda Química de Oxígeno, DQO y nutrientes (nitrógeno (N-NH4+, N-NO3- y NT y fósforo (P-PO4-3 y PT. Las unidades de HSS plantadas con Phragmites australis mostraron mejor adaptación temprana que las unidades de HSS plantadas con Schoenoplectus californicus. Sin embargo, las eficiencias de eliminación fueron similares en todas las unidades. Estacionalmente, se pudo evidenciar una diferencia de eliminación para DQO (invierno: 18 a 30%, primavera: 45 a 55% y NT (invierno: 25 a 65%, primavera: 25 a 35%.

  8. Biological Flora of the British Isles: Phragmites australis

    Czech Academy of Sciences Publication Activity Database

    Packer, J. G.; Meyerson, L. A.; Skálová, Hana; Pyšek, Petr; Kueffer, C.

    2017-01-01

    Roč. 105, č. 4 (2017), s. 1123-1162 ISSN 0022-0477 R&D Projects: GA ČR(CZ) GA14-15414S Grant - others:AV ČR(CZ) AP1002 Program:Akademická prémie - Praemium Academiae Institutional support: RVO:67985939 Keywords : common reed * genome size * plant invasion Subject RIV: EF - Botanics OBOR OECD: Plant sciences, botany Impact factor: 5.813, year: 2016

  9. Phragmites australis as a model organism for studying plant invasions

    Czech Academy of Sciences Publication Activity Database

    Meyerson, L. A.; Cronin, J. T.; Pyšek, Petr

    2016-01-01

    Roč. 18, č. 9 (2016), s. 2421-2431 ISSN 1387-3547 R&D Projects: GA ČR(CZ) GA14-15414S Grant - others:AV ČR(CZ) AP1002 Program:Akademická prémie - Praemium Academiae Institutional support: RVO:67985939 Keywords : common reed * model species * global climate change Subject RIV: EH - Ecology, Behaviour Impact factor: 2.473, year: 2016

  10. Automatic Dissection Of Plantlets

    Science.gov (United States)

    Batchelor, B. G.; Harris, I. P.; Marchant, J. A.; Tillett, R. D.

    1989-03-01

    Micropropagation is a technique used in horticulture for generating a monoclonal colony of plants. A tiny plantlet is cut into several parts, each of which is then replanted. At the moment, the cutting is performed manually. Automating this task would have significant economic benefits. A robot designed to dissect plants would need to be equipped with intelligent visual sensing. This article is concerned with the image acquisition and processing techniques which such a machine might use. A program, which can calculate where to cut a plant with an "open" structure, is presented. This is expressed in the ProVision language, which is described in another article presented at this conference. (Article 1002-65)

  11. Australis: Progress Report

    Energy Technology Data Exchange (ETDEWEB)

    Sie, S.H.; Niklaus, T.R.; Suter, G.F. [Commonwealth Scientific and Industrial Research Organisation (CSIRO), North Ryde, NSW (Australia). Div. of Exploration Geoscience

    1996-12-31

    The first stage of the development of AUSTRALIS, a microbeam AMS system at the HIGF laboratory at North Ryde, Sydney has been completed. The system is designed to enable in-situ microanalysis of geological samples for ultra-traces and for isotopic data for minerals exploration research. The negative ions for analysis are produced by sputtering with a microbeam of Cs{sup +}from a modified General Ionex model 834 HICONEX ion source. The source features a novel intermediate or `screen` electrode to correct for the effect of the secondary ion extraction field on the trajectory of the primary beam,in order to bring the primary beam to the geometric centre. The high energy analysis system of AUSTRALIS features a pair of deflector systems to permit fast switching of isotopes without altering the magnet setting. The paper describes the initial tests` results showing good agreement with the design parameters. 5 refs., 4 figs.

  12. Australis: Progress Report

    International Nuclear Information System (INIS)

    Sie, S.H.; Niklaus, T.R.; Suter, G.F.

    1996-01-01

    The first stage of the development of AUSTRALIS, a microbeam AMS system at the HIGF laboratory at North Ryde, Sydney has been completed. The system is designed to enable in-situ microanalysis of geological samples for ultra-traces and for isotopic data for minerals exploration research. The negative ions for analysis are produced by sputtering with a microbeam of Cs + from a modified General Ionex model 834 HICONEX ion source. The source features a novel intermediate or 'screen' electrode to correct for the effect of the secondary ion extraction field on the trajectory of the primary beam,in order to bring the primary beam to the geometric centre. The high energy analysis system of AUSTRALIS features a pair of deflector systems to permit fast switching of isotopes without altering the magnet setting. The paper describes the initial tests' results showing good agreement with the design parameters. 5 refs., 4 figs

  13. Australis: Progress Report

    Energy Technology Data Exchange (ETDEWEB)

    Sie, S H; Niklaus, T R; Suter, G F [Commonwealth Scientific and Industrial Research Organisation (CSIRO), North Ryde, NSW (Australia). Div. of Exploration Geoscience

    1997-12-31

    The first stage of the development of AUSTRALIS, a microbeam AMS system at the HIGF laboratory at North Ryde, Sydney has been completed. The system is designed to enable in-situ microanalysis of geological samples for ultra-traces and for isotopic data for minerals exploration research. The negative ions for analysis are produced by sputtering with a microbeam of Cs{sup +}from a modified General Ionex model 834 HICONEX ion source. The source features a novel intermediate or `screen` electrode to correct for the effect of the secondary ion extraction field on the trajectory of the primary beam,in order to bring the primary beam to the geometric centre. The high energy analysis system of AUSTRALIS features a pair of deflector systems to permit fast switching of isotopes without altering the magnet setting. The paper describes the initial tests` results showing good agreement with the design parameters. 5 refs., 4 figs.

  14. Somatic embryogenesis and plantlet regeneration from protoplast ...

    African Journals Online (AJOL)

    Administrator

    2010-05-30

    May 30, 2010 ... supplemented with 1 mg/l each of NAA and BA, 100 mg/l ascorbic acid and 0.5 M mannitol at 25°C in ... Key words: Ca-alginate beads, Muscari neglectum, nurse culture, plantlet regeneration, ..... Maddock SR (1987).

  15. Advancing the science of microbial symbiosis to support invasive species management: a case study on Phragmites in the Great Lakes.

    Science.gov (United States)

    Kowalski, Kurt P; Bacon, Charles; Bickford, Wesley; Braun, Heather; Clay, Keith; Leduc-Lapierre, Michèle; Lillard, Elizabeth; McCormick, Melissa K; Nelson, Eric; Torres, Monica; White, James; Wilcox, Douglas A

    2015-01-01

    A growing body of literature supports microbial symbiosis as a foundational principle for the competitive success of invasive plant species. Further exploration of the relationships between invasive species and their associated microbiomes, as well as the interactions with the microbiomes of native species, can lead to key new insights into invasive success and potentially new and effective control approaches. In this manuscript, we review microbial relationships with plants, outline steps necessary to develop invasive species control strategies that are based on those relationships, and use the invasive plant species Phragmites australis (common reed) as an example of how development of microbial-based control strategies can be enhanced using a collective impact approach. The proposed science agenda, developed by the Collaborative for Microbial Symbiosis and Phragmites Management, contains a foundation of sequential steps and mutually-reinforcing tasks to guide the development of microbial-based control strategies for Phragmites and other invasive species. Just as the science of plant-microbial symbiosis can be transferred for use in other invasive species, so too can the model of collective impact be applied to other avenues of research and management.

  16. Advancing the science of microbial symbiosis to support invasive species management: a case study on Phragmites in the Great Lakes

    Science.gov (United States)

    Kowalski, Kurt P.; Bacon, Charles R.; Bickford, Wesley A.; Braun, Heather A.; Clay, Keith; Leduc-Lapierre, Michele; Lillard, Elizabeth; McCormick, Melissa K.; Nelson, Eric; Torres, Monica; White, James W. C.; Wilcox, Douglas A.

    2015-01-01

    A growing body of literature supports microbial symbiosis as a foundational principle for the competitive success of invasive plant species. Further exploration of the relationships between invasive species and their associated microbiomes, as well as the interactions with the microbiomes of native species, can lead to key new insights into invasive success and potentially new and effective control approaches. In this manuscript, we review microbial relationships with plants, outline steps necessary to develop invasive species control strategies that are based on those relationships, and use the invasive plant species Phragmites australis (common reed) as an example of how development of microbial-based control strategies can be enhanced using a collective impact approach. The proposed science agenda, developed by the Collaborative for Microbial Symbiosis andPhragmites Management, contains a foundation of sequential steps and mutually-reinforcing tasks to guide the development of microbial-based control strategies for Phragmites and other invasive species. Just as the science of plant-microbial symbiosis can be transferred for use in other invasive species, so too can the model of collective impact be applied to other avenues of research and management.

  17. Monitoring Spatial Variability and Temporal Dynamics of Phragmites Using Unmanned Aerial Vehicles

    Directory of Open Access Journals (Sweden)

    Viktor R. Tóth

    2018-06-01

    Full Text Available Littoral zones of freshwater lakes are exposed to environmental impacts from both terrestrial and aquatic sides, while substantial anthropogenic pressure also affects the high spatial, and temporal variability of the ecotone. In this study, the possibility of monitoring seasonal and spatial changes in reed (Phragmites australis stands using an unmanned aerial vehicle (UAV based remote sensing technique was examined. Stands in eutrophic and mesotrophic parts of Lake Balaton including not deteriorating (stable and deteriorating (die-back patches, were tracked throughout the growing season using a UAV equipped with a Normalized Difference Vegetation Index (NDVI camera. Photophysiological parameters of P. australis were also measured with amplitude modulated fluorescence. Parameters characterizing the dynamics of seasonal changes in NDVI data were used for phenological comparison of eutrophic and mesotrophic, stable and die-back, terrestrial and aquatic, mowed and not-mowed patches of reed. It was shown that stable Phragmites plants from the eutrophic part of the lake reached specific phenological stages up to 3.5 days earlier than plants from the mesotrophic part of the lake. The phenological changes correlated with trophic (total and nitrate-nitrite nitrogen and physical (organic C and clay content properties of the sediment, while only minor relationships with air and water temperature were found. Phenological differences between the stable and die-back stands were even more pronounced, with ~34% higher rates of NDVI increase in stable than die-back patches, while the period of NDVI increase was 16 days longer. Aquatic and terrestrial parts of reed stands showed no phenological differences, although intermediate areas (shallow water parts of stands were found to be less vigorous. Winter mowing of dried Phragmites sped up sprouting and growth of reed in the spring. This study showed that remote sensing-derived photophysiological and phenological

  18. Plasticity of Lythrum salicaria and Phragmites australis growth characteristics across a European geographical gradient

    Czech Academy of Sciences Publication Activity Database

    Bastlová, D.; Bastl, M.; Čížková, Hana; Květ, Jan

    2006-01-01

    Roč. 570, - (2006), s. 237-242 ISSN 0018-8158 R&D Projects: GA ČR(CZ) GA206/00/1113; GA AV ČR(CZ) KJB6141403 Institutional research plan: CEZ:AV0Z60870520; CEZ:AV0Z60050516 Keywords : life history * flowering time * competive ability * plant invasions * geographic variation * clinal variation Subject RIV: EF - Botanics Impact factor: 1.049, year: 2006

  19. Metabolism of carbamazepine in plant roots and endophytic rhizobacteria isolated from Phragmites australis.

    Science.gov (United States)

    Sauvêtre, Andrés; May, Robert; Harpaintner, Rudolf; Poschenrieder, Charlotte; Schröder, Peter

    2018-01-15

    Carbamazepine (CBZ) is a pharmaceutical frequently categorized as a recalcitrant pollutant in the aquatic environment. Endophytic bacteria previously isolated from reed plants have shown the ability to promote growth of their host and to contribute to CBZ metabolism. In this work, a horseradish (Armoracia rusticana) hairy root (HR) culture has been used as a plant model to study the interactions between roots and endophytic bacteria in response to CBZ exposure. HRs could remove up to 5% of the initial CBZ concentration when they were grown in spiked Murashige and Skoog (MS) medium. Higher removal rates were observed when HRs were inoculated with the endophytic bacteria Rhizobium radiobacter (21%) and Diaphorobacter nitroreducens (10%). Transformation products resulting from CBZ degradation were identified using liquid chromatography-ultra high-resolution quadrupole time of flight mass spectrometry (LC-UHR-QTOF-MS). CBZ metabolism could be divided in four pathways. Metabolites involving GSH conjugation and 2,3-dihydroxylation, as well as acridine related compounds are described in plants for the first time. This study presents strong evidence that xenobiotic metabolism and degradation pathways in plants can be modulated by the interaction with their endophytic community. Hence it points to plausible applications for the elimination of recalcitrant compounds such as CBZ from wastewater in CWs. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Genetic diversity and dispersal of Phragmites australis in a small river system

    Czech Academy of Sciences Publication Activity Database

    Fér, T.; Hroudová, Zdenka

    2009-01-01

    Roč. 90, č. 2 (2009), s. 165-171 ISSN 0304-3770 R&D Projects: GA AV ČR KJB6111304 Institutional research plan: CEZ:AV0Z60050516 Keywords : genetic variation * long-distance dispersal * spatial autocorrelation Subject RIV: EF - Botanics Impact factor: 1.697, year: 2009

  1. Bioaccumulation of selected metals in bivalves (Unionidae) and Phragmites australis inhabiting a municipal water reservoir

    OpenAIRE

    Rzymski, Piotr; Niedzielski, Przemysław; Klimaszyk, Piotr; Poniedziałek, Barbara

    2014-01-01

    Urbanization can considerably affect water reservoirs by, inter alia, input, and accumulation of contaminants including metals. Located in the course of River Cybina, Maltański Reservoir (Western Poland) is an artificial shallow water body built for recreation and sport purposes which undergoes restoration treatment (drainage) every 4 years. In the present study, we demonstrate an accumulation of nine metals (Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, Zn) in water, sediment, three bivalve species (Anodo...

  2. Cytokinin dynamics in differently senescing laminae of Phragmites australis plants grown in different habitats

    Czech Academy of Sciences Publication Activity Database

    Conrad, K.; Motyka, Václav; Bernhardt, R.; Stein, C.

    2015-01-01

    Roč. 99, JUL 2015 (2015), s. 54-61 ISSN 0254-6299 R&D Projects: GA ČR(CZ) GAP506/11/0774 Institutional support: RVO:61389030 Keywords : Chloride * Chlorophylls * Common reed Subject RIV: EF - Botanics Impact factor: 1.244, year: 2015

  3. Recloning of regenerated plantlets from elite oil palm ( Elaeis ...

    African Journals Online (AJOL)

    Plant regeneration in oil palm cv. Tenera via somatic embryogenesis was conducted using regenerated plantlets as an explant source. Explants from different positions – apex, middle and basal segments of regenerated plantlets – were cultured in N6 medium supplemented with 100, 120 and 140 mg/L 2 ...

  4. Acclimatization and leaf anatomy of micropropagated fig plantlets

    Directory of Open Access Journals (Sweden)

    Chrystiane Fráguas Chirinéa

    2012-12-01

    Full Text Available The survival of micropropagated plants during and after acclimatization is a limiting process to plant establishment. There is little information on how the anatomy of vegetative organs of Ficus carica can be affected by culture conditions and acclimatization. The present research aimed to study the effects of time on culture medium and substrates during the acclimatization of fig tree plantlets produced in vitro, characterizing some leaf anatomy aspects of plantlets cultured in vitro and of fig trees produced in field. Plantlets previously multiplied in vitro were separated and transferred into Wood Plant Medium (WPM where they were kept for 0, 15, 30, 45 and 60 days. Different substrates were tested and studies on leaf anatomy were performed in order to compare among plantlets grown in vitro, plantlets under 20, 40 and 60 days of acclimatization, and field grown plants. Keeping plantlets for 30 days in WPM allowed better development in Plantmax during acclimatization. Field grown plants presented higher number of stomata, greater epicuticular wax thickness and greater leaf tissue production compared to in vitro ones. The leaf tissues of in vitro plantlets show little differentiation and have great stomata number compared with acclimatized plants, which reduce the number of stomata during the acclimatization process.

  5. Organelles genome stability of wheat plantlets produced by anther ...

    African Journals Online (AJOL)

    Yomi

    2012-03-15

    Mar 15, 2012 ... 1Department of Biotechnology, Faculty of Agricultural Technology, Al-Balqa' Applied University, ... genetic stability of wheat organelles genomes for plantlets produced by anther culture using restriction ..... of transgenic plants.

  6. Enhancement of Diosgenin Production in Plantlet and Cell Cultures ...

    African Journals Online (AJOL)

    Enhancement of Diosgenin Production in Plantlet and Cell Cultures of Dioscorea zingiberensis by Palmarumycin C13 from the Endophytic fungus, Berkleasmium sp. Dzf12. Y Mou, K Zhou, D Xu, R Yu, J Li, C Yin, L Zhou ...

  7. Reproduction at the extremes: pseudovivipary, hybridization and genetic mosaicism in Posidonia australis (Posidoniaceae).

    Science.gov (United States)

    Sinclair, Elizabeth A; Statton, John; Hovey, Renae; Anthony, Janet M; Dixon, Kingsley W; Kendrick, Gary A

    2016-02-01

    Organisms occupying the edges of natural geographical ranges usually survive at the extreme limits of their innate physiological tolerances. Extreme and prolonged fluctuations in environmental conditions, often associated with climate change and exacerbated at species' geographical range edges, are known to trigger alternative responses in reproduction. This study reports the first observations of adventitious inflorescence-derived plantlet formation in the marine angiosperm Posidonia australis, growing at the northern range edge (upper thermal and salinity tolerance) in Shark Bay, Western Australia. These novel plantlets are described and a combination of microsatellite DNA markers and flow cytometry is used to determine their origin. Polymorphic microsatellite DNA markers were used to generate multilocus genotypes to determine the origin of the adventitious inflorescence-derived plantlets. Ploidy and genome size were estimated using flow cytometry. All adventitious plantlets were genetically identical to the maternal plant and were therefore the product of a novel pseudoviviparous reproductive event. It was found that 87 % of the multilocus genotypes contained three alleles in at least one locus. Ploidy was identical in all sampled plants. The genome size (2 C value) for samples from Shark Bay and from a separate site much further south was not significantly different, implying they are the same ploidy level and ruling out a complete genome duplication (polyploidy). Survival at range edges often sees the development of novel responses in the struggle for survival and reproduction. This study documents a physiological response at the trailing edge, whereby reproductive strategy can adapt to fluctuating conditions and suggests that the lower-than-usual water temperature triggered unfertilized inflorescences to 'switch' to growing plantlets that were adventitious clones of their maternal parent. This may have important long-term implications as both genetic and

  8. In vitro regeneration of hybrid plantlets of cashew (Anacardium ...

    African Journals Online (AJOL)

    Embryos from immature nuts of cashew (Anacardium occidentale L.) were cultured in vitro to regenerate improved hybrid plantlets. Explants (embryo) were excised from developing F1 hybrid immature nuts derived from diallel cross and harvested at 2-, 4-, 6- and 8-weeks after pollination (WAPo) for in vitro culture.

  9. Regeneration of plantlets from unpollinated ovary cultures of ...

    African Journals Online (AJOL)

    An in vitro culture protocol was established for direct regeneration of plantlets from unpollinated ovary cultures of four Ethiopian wheat varieties. Unpollinated ovaries were excised from durum wheat (Yerer and Ude varieties) and bread wheat (Simba and Galama varieties). Analysis of variance (ANOVA) has shown that ...

  10. Eucalyptus plantlet growth in relation to foliar application with ...

    African Journals Online (AJOL)

    In one experiment at greenhouse, condition was established to determine the effects of complete fertilizers on growth of plantlet Eucalyptus camaldulensis in Southeast of Iran. This experiment was conducted in the Agricultural Research Center, University of Zabol, in autumn year of 2008. This study was designed as a ...

  11. Phragmites Management at Times Beach, Buffalo, New York

    Science.gov (United States)

    2016-01-01

    nettle Urtica dioica L. ssp dioica N RESULTS AND DISCUSSION: Baseline data collected in summer 2012 was used to characterize the site (Table 3...because eighty percent of phragmites biomass is produced underground in the roots and rhizomes (Holm et al. 1977), and rhizomes can live for three to six

  12. Biotransformation of benzimidazole anthelmintics in reed (Phragmites australis) as a potential tool for their detoxification in environment

    Czech Academy of Sciences Publication Activity Database

    Podlipná, Radka; Skálová, L.; Seidlová, H.; Szotáková, B.; Kubíček, V.; Stuchlíková, L.; Jirásko, R.; Vaněk, Tomáš; Vokřál, I.

    2013-01-01

    Roč. 144, č. 2013 (2013), s. 216-224 ISSN 0960-8524 R&D Projects: GA TA ČR TA01020573 Institutional research plan: CEZ:AV0Z50380511 Keywords : Albendazole * Phytotransformation * Drug-metabolism Subject RIV: DJ - Water Pollution ; Quality Impact factor: 5.039, year: 2013

  13. Jack-and-Master Trait Responses to Elevated CO2 and N: A Comparison of Native and Introduced Phragmites australis

    OpenAIRE

    Mozdzer, Thomas J.; Megonigal, J. Patrick

    2012-01-01

    Global change is predicted to promote plant invasions world-wide, reducing biodiversity and ecosystem function. Phenotypic plasticity may influence the ability of introduced plant species to invade and dominate extant communities. However, interpreting differences in plasticity can be confounded by phylogenetic differences in morphology and physiology. Here we present a novel case investigating the role of fitness trait values and phenotypic plasticity to global change factors between conspec...

  14. Supplemental Enviromnentul Assessment for Control of Common Reed (Phragmites australis) at Joint Base Langley-Eustis, Fort Eustis, Virginia

    Science.gov (United States)

    2012-09-01

    in 2009 northwest of Third Port on a portion of land owned by DOD, but is not uti !iz~d by military unit~. This n~st r~main~ active. A potential...glyphosate orally during pregnancy . evo11 at amounts wl1iGh producOO advers~ effects on the mol hers. Glyphosale was fed continuously to rats at very high

  15. Phenotypic traits of the Mediterranean Phragmites australis M1 lineage: differences between the native and introduced ranges

    Czech Academy of Sciences Publication Activity Database

    Guo, Wen-Yong; Lambertini, C.; Guo, X.; Li, X.-Z.; Eller, F.; Brix, H.

    2016-01-01

    Roč. 18, č. 9 (2016), s. 2551-2561 ISSN 1387-3547 Institutional support: RVO:67985939 Keywords : comon reed * delta type * ecological fitting Subject RIV: EH - Ecology, Behaviour Impact factor: 2.473, year: 2016

  16. Plantlets from encapsulated shoot buds of Catalpa ovata G. Don

    Directory of Open Access Journals (Sweden)

    Halina Wysokińska

    2014-01-01

    Full Text Available Shoot buds isolated from in vitro shoot cultures of Catalpa ovata G. Don were encapsulated using 3% sodium alginate with sucrose (3% and 50 mM calcium chloride. The morphogenic response of encapsulated buds was affected by such factors, like composition of the media and the presence of growth regulators. The highest frequency of plantlet germination from encapsulated buds (70% within 4 weeks was obtained on Woody Plant medium (WP (Lloyd and McCown 1980 containing indole-3-butyric acid (IBA (1 mg/l. The process was substantially inhibited by cold-storage (4oC of encapsulated buds. In this case, the frequency response ranged from 3% to 22% dependent on storage period (28 or 42 days and the presence of the paraffin coat covering the alginate capsules. The plantlets developed from both unstored and stored encapsulated buds of C. ovata were transplanted to soil and grew in pots to phenotypically normal plants.

  17. Aluminium stress disrupts metabolic performance of Plantago almogravensis plantlets transiently.

    Science.gov (United States)

    Grevenstuk, Tomás; Moing, Annick; Maucourt, Mickaël; Deborde, Catherine; Romano, Anabela

    2015-12-01

    Little is known about how tolerant plants cope with internalized aluminium (Al). Tolerant plants are known to deploy efficient detoxification mechanisms, however it is not known to what extent the primary and secondary metabolism is affected by Al. The aim of this work was to study the metabolic repercussions of Al stress in the tolerant plant Plantago almogravensis. P. almogravensis is well adapted to acid soils where high concentrations of free Al are found and has been classified as a hyperaccumulator. In vitro reared plantlets were used for this purpose in order to control Al exposure rigorously. The metabolome of P. almogravensis plantlets as well as its metabolic response to the supply of sucrose was characterized. The supply of sucrose leads to an accumulation of amino acids and secondary metabolites and consumption of carbohydrates that result from increased metabolic activity. In Al-treated plantlets the synthesis of amino acids and secondary metabolites is transiently impaired, suggesting that P. almogravensis is able to recover from the Al treatment within the duration of the trials. In the presence of Al the consumption of carbohydrate resources is accelerated. The content of some metabolic stress markers also demonstrates that P. almogravensis is highly adapted to Al stress.

  18. JST Thesaurus Headwords and Synonyms: Phragmites communis [MeCab user dictionary for science technology term[Archive

    Lifescience Database Archive (English)

    Full Text Available MeCab user dictionary for science technology term Phragmites communis 名詞 一般 * * * *... ヨシ ヨシ ヨシ Thesaurus2015 200906077254295905 C LS06 UNKNOWN_2 Phragmites communis

  19. Research Ship Aurora Australis Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Research Ship Aurora Australis Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic...

  20. Effects of chronic gamma irradiation on adventitious plantlet formation of Saintpaulia ionantha (African violet) detached leaves

    International Nuclear Information System (INIS)

    Arunee Wongpiyasatid; Peeranuch Jompuk; Katarat Chusreeaeom; Thanya Taychasinpitak

    2007-01-01

    Formation of adventitious plantlets on unrootedly detached leaves of two African violet (Saintpaulia ionantha) cultivars, pink and violet flowers, chronically gamma-irradiated in gamma room at The Gamma Irradiation Service and Nuclear Technology Research Center, Kasetsart University was compared. Detached leaves were immediately planted after detachment in plastic trays containing peat moss, 18 leaves per treatment with 3 replications. Three dose rates (rad/h) with 3 doses (rad)/dose rate, were applied to the irradiated samples while the controls were placed outside the gamma room. Three months after irradiation, the number of survived leaves, the number of leaves producing adventitious plantlets and the number of plantlets per leaf were recorded. After that, the young plantlets were transferred to the new pots for further observation on plant growth and mutation characters. The results revealed that the number of survived leaves, the number of leaves producing adventitious plantlets and the number of plantlets per leaf varied slightly with radiation doses but were not significantly different at different dose rates. Radiosensitivity was noticed to be higher in pink flower cultivar than the violet one. M 1 V 1 plantlets will be followed up for growth and mutation character observations

  1. Over-expression of KdSOC1 gene affected plantlet morphogenesis in Kalanchoe daigremontiana.

    Science.gov (United States)

    Zhu, Chen; Wang, Li; Chen, Jinhua; Liu, Chenglan; Zeng, Huiming; Wang, Huafang

    2017-07-17

    Kalanchoe daigremontiana reproduces asexually by producing plantlets along the leaf margin. The aim of this study was to identify the function of the SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 gene in Kalanchoe daigremontiana (KdSOC1) during plantlet morphogenesis. In this study, KdSOC1 gene expression was detected at stem cell niche during in vitro somatic embryogenesis and plantlet morphogenesis. Disrupting endogenous auxin transportation suppressed the KdSOC1 gene response. Knockdown of the KdSOC1 gene caused a defect in cotyledon formation during the early heart stage of somatic embryogenesis. Over-expression (OE) of the KdSOC1 gene resulted in asymmetric plantlet distribution, a reduced number of plantlets, thicker leaves, and thicker vascular fibers. Higher KdPIN1 gene expression and auxin content were found in OE plant compared to those of wild-type plant leaves, which indicated possible KdSOC1 gene role in affecting auxin distribution and accumulation. KdSOC1 gene OE in DR5-GUS Arabidopsis reporting lines resulted in an abnormal auxin response pattern during different stages of somatic embryogenesis. In summary, the KdSOC1 gene OE might alter auxin distribution and accumulation along leaf margin to initiate plantlet formation and distribution, which is crucial for plasticity during plantlet formation under various environmental conditions.

  2. Plantlet regeneration potential from seedling explants of vitegnus (Vitex agnus castus).

    Science.gov (United States)

    Chamandoosti, F

    2007-11-15

    In this research a simple and repeatable method for regeneration of a important medicinal plant (Vitex agnus castus) described. Different seedling explants such as hypocotyl, cotyledon, root and apical meristem were cultured in MS basal media with different kinds and concentrations of PGRs. Root and apical meristem explants were the only explants that have regeneration whole plantlets potential. It was interesting that regeneration whole plantlets from root and apical meristem explants have different developmental pathways. Whole plantlets from apical meristem explants regenerated by passing phase callusing whereas regeneration whole plantlets from root was direct and without phase callusing. This subject implies that we can have many manipulation possibilities in order to different objects of tissue culture by selecting different explants in vitegnus.

  3. Using the "Kalanchoe daigremontiana" Plant To Show the Effects of Photoperiodism on Plantlet Formation.

    Science.gov (United States)

    Hershey, David R.

    2002-01-01

    Describes an activity demonstrating the importance of photoperiod on plant development. Uses the plant devil's backbone for the experiment and studies the details of photoperiodic requirement for plantlet formation. (Contains 12 references.) (YDS)

  4. [Study on Rapid Micropropagation in Vitro Technique of Guangfeng Medicinal Yam (Dioscorea opposita) Plantlets].

    Science.gov (United States)

    Yin, Ming-Hua; Xu, Zhi-Jian; Zhang, Sheng-qin; Lv, Si-Jie; Zeng, Yan-hong; Fu, You-zhang; Hong, Sen-rong

    2015-11-01

    In order to provide methodology reference for virus-free and germplasm conservation of Guangfeng medicinal yam (Dioscorea opposita) plantlets, rapid micropropagation in vitro technique of Guangfeng medicinal yam plantlets was studied. Using the method of plant tissue culture, single factor test and flow-cytometry, the basic procedure of Guangfeng medicinal yam tissue culture was established and the DNA content of Guangfeng medicinal yam plantlets and its potted seedlings was detected. The best disinfection procedure of stems with a bud of Guangfeng medicinal yam was washed with sterile water for three times after sterilized with 70% alcohol for 20 - 30 s and then washed with sterile water for three times again after sterilized with 0.1% mercuric chloride for 10 - 12 min; The best explants of stems with a bud of Guangfeng medicinal yam was slightly woody and more mature stems witha bud; The best proliferation culture medium of stems with a bud of Guangfeng medicinal yam was MS + 6-BA 2.0 mg/L + NAA 0.1 mg/L; The best rooting culture medium of stems with a bud of Guangfeng medicinal yam was MS + NAA 0.5 mg/L; The best culture method of Guangfeng medicinal yam plantlets was liquid culture; The best transplanting matrix of Guangfeng medicinal yam plantlets was the mixture of paddy clay and fine sand (1: 2) or the mixture of perlite and vermiculite (1: 2); The DNA content between Guangfeng medicinal yam plantlets and its potted seedlings had no significant difference. A fast and efficient micropropagation in vitro technological system of stems with a bud of Guangfeng medicinal yam is established, and the flow cytometry detect results also show the genetic stability of Guangfeng medicinal yam plantlets, whose results provide the technical and theoretical basis for the large-scale production of Guangfeng medicinal yam plantlets.

  5. Development and growth of plantlets of Pinus contorta regenerated from adventitious buds

    Energy Technology Data Exchange (ETDEWEB)

    Flygh, G.; Groenroos, R.; Arnold, S. von [Swedish Univ. of Agricultural Sciences, Uppsala (Sweden). Dept. of Forest Genetics; Hoegberg, K.A. [The Association for Forest Tree Breeding, Svaloev (Sweden)

    1998-11-01

    Before micropropagation techniques can be applied to a particular species, it is crucial to optimize the method and to determine how the micropropagated plants grow in the field. Adventitious shoots developed on embryos of Pinus contorta Dougl. ex Loud. after a 2 h pulse treatment with 250 {mu}M N6-benzyladenine. The time to first subculture after the pulse treatment influenced the yield of adventitious shoots. On average, 68% of the adventitious shoots had developed roots 12 weeks after treatment with 1.25 mM indole-3-butyric acid for 6 h. The auxin treatment stimulated early rooting (i.e. within 6 weeks) but had no effect on late rooting (i.e. after 6 weeks). The size of the plantlets was of importance for the survival when potted. All plantlets with a distinct stem elongated during the first growth period while some without did not. The relative height growth rate of plantlets was similar to that of seedlings. In the field the increase of height was similar for plantlets and seedlings. Plagiotropy was higher for the plantlets than for the seedlings (35 and 10% respectively). We concluded that most plantlets of P. contorta elongate normally and have a similar gross morphology to seedlings 23 refs, 8 figs, 4 tabs

  6. Organ and plantlet regeneration of Menyanthes trifoliata through tissue culture

    Directory of Open Access Journals (Sweden)

    Urszula Adamczyk-Rogozińska

    2014-01-01

    Full Text Available The conditions for the regeneration of plants through organogenesis from callus tissues of Menyanthes trifoliata are described. The shoot multiplication rate was affected by basal culture media, the type and concentration of cytokinin and subculture number. The best response was obtained when caulogenic calli were cultured on the modified Schenk and Hildebrandt medium (SH-M containing indole-3-acetic acid (IAA 0,5 mg/l and 6-benzyladenine (BA 1 mg/l or zeatin (2 mg/l. Under these conditions ca 7 shoots (mostly 1 cm or more in length per culture in the 5th and 6th passages could be developed. In older cultures (after 11-12 passages there was a trend for more numerous but shorter shoot formation. All regenerated shoots could be rooted on the SH-M medium supplemented with 0.5 mg/l IAA within 6 weeks; 80% of in vitro rooted plantlets survived their transfer to soil.

  7. Pemanfataan Pupuk Daun, Air Kelapa dan Bubur Pisang sebagai Komponen Medium Pertumbuhan Plantlet Anggrek Dendrobium Kelemense

    Directory of Open Access Journals (Sweden)

    Uhwatul Hasanah

    2014-09-01

    Full Text Available Bahan alternatif alami diperlukan untuk menggantikan bahan kimia yang mahal untuk kegiatan kultur jaringan. Penelitian ini bertujuan untuk menguji pengaruh merk, konsentrasi pupuk daun, interaksinya terhadap pertumbuhan plantlet anggrek Dendrobium dan menentukan konsentrasi yang paling optimal dalam menginduksi pertumbuhan plantlet. Percobaan dilakukan dengan rancangan acak lengkap faktorial yang terdiri dari dua perlakuan yaitu merk pupuk dan konsentrasi pupuk, masing-masing dengan tiga taraf perlakuan yaitu merk pupuk (growmore, hyponex, gandasil dan konsentrasi (1 g/l, 2 g/l, 3 g/l. Pertumbuhan plantlet anggrek pada penelitian ini diukur berdasarkan parameter tinggi plantlet, jumlah daun, luas daun, jumlah akar dan panjang akar. Data dianalisis dengan anava dua arah, bila signifikan dilanjutkan dengan uji Duncan. Hasil anava menunjukkan merk pupuk berpengaruh signifikan terhadap penambahan jumlah daun, luas daun dan jumlah akar, konsentrasi pupuk berpengaruh signifikan terhadap penambahan tinggi plantlet dan panjang akar sedangkan interaksi merk pupuk dengan konsentrasi pupuk signifikan terhadap penambahan tinggi plantlet dan luas daun. Kombinasi perlakuan yang paling optimal yang menginduksi penambahan tinggi plantlet dan luas daun adalah pupuk hyponex dengan konsentrasi 2 g/l (5,40 cm dan 5,43 cm2. Untuk mendapatkan pertumbuhan plantlet paling tinggi dan luas daun paling optimal digunakan media pupuk hyponex dengan konsentrasi pupuk 2 g/l.Natural alternative materials needed to replace expensive chemicals for tissue culture activities. This study aimed to examine the influence of the brand, the concentration of foliar fertilizer, interaction on the growth of dendrobium orchid Dendrobium and determine the optimal concentration in inducing the growth of plantlets. Experiments were carried out with completely randomized factorial design consisting of two treatments, the brand of fertilizer and manure concentration, each with three levels

  8. BOOK REVIEW: European Perceptions of Terra Australis

    Science.gov (United States)

    Sterken, Christiaan

    2012-12-01

    Terra Australis - the southern land - has been one of the most widespread concepts in European geography from the sixteenth to the eighteenth century. This book comprises a set of 14 interdisciplinary scholarly contributions that deal with personal perceptions of Terra Australis by cartographers and explorers, and with putting these perceptions in their historical and cultural environments. This book seems, at a first glance, to be very remote from astronomy - and even from the history of astronomy - however, as it also offers an excellent background to Captain James Cook's second voyage to observe the 1769 transit of Venus from Tahiti, it definitely is a work of truly interdisciplinary character. Cook's voyages, in fact, became a model in which key scientists of many nationalities and disciplines traveled together on ships. In these voyages, art, science, technology and political power were centralised and united. The chapters range across history, the visual arts, literature, popular culture, technology, politics and science. Issues of scientific reasoning are raised in the description of how people did think about the south before there even existed a perception of the unknown land - quite comparable to how ancient and early-modern astronomers had their thought about cosmology even before any observational data were available. Several early map systems - like the zonal and T-O maps (medieval world maps with the letter T inside an O representing the lands inside a circle of oceans) - are described, and the description of Roman geography shows the amazing fact that theory and practice were not unified, and existed independently of each other insofar that a real paradox between theory and observation had persisted for a very long time. The maps and charts also exemplify the long-lasting consequences of early modern copy-paste practice: navigators copied original sketch charts of coasts that were previously unknown to them, herewith committing many translation and

  9. Salinity-Induced Callus Browning and Re-Differentiation, Root Formation by Plantlets and Anatomical Structures of Plantlet Leaves in Two Malus Species

    International Nuclear Information System (INIS)

    Gou, W.; Zheng, P.; Zheng, P.; Wang, K.; Zhang, L.; Akram, N. A.

    2016-01-01

    Apple (Malus domestica L.) is widely grown in northern China. However, soil salinization has become one of the most severe factors limiting apple productivity in some regions including the Loess Plateau. In our study, the regeneration system of both rootstock Rehd (Malus robusta Rehd) and scion Fuji (Malus domestica Borkh. cv. Fuji) was established In vitro. The two Malus species were cultured on the MS medium containing 0 or 150 mM NaCl to examine salt-induced effects on callus browning and re-differentiation, root formation of plantlets and anatomical structures of plantlet leaves at 15 days old callus and plantlet stages. Salt stress caused a marked increase in callus browning rate, while a decrease in re-differentiation rate, rooting rate, root number and length in both species. Additionally, anatomical structures of plantlet leave showed salt-induced damage such as reduced palisade tissue and intracellular chloroplast, incomplete development of xylem and severe damage of the phloem tissue. Salt stress also caused a few adaptive structural features in leaves including increased thickness of upper and lower epidermis, elevated proportion of spongy tissue and formation of lignified vessels. The responses of the two Malus species did not differ significantly at the differentiation stage. However, they were more sensitive to salinity at the callus stage than those at the plantlet stage in each species. Therefore, callus stage has been found to be more suitable for evaluating responses of the two apple species to salt stress. The Fuji and Rehd could be treated as a good scion/rootstock combination of apple to adapt to soil salinity based on their similar degree of salt stress-tolerance. (author)

  10. Packaging of Post Acclimatized Somatic Embryogenesis Cocoa Plantlet (Theobroma cacao L.

    Directory of Open Access Journals (Sweden)

    Soedarsianto Soedarsianto

    2009-05-01

    Full Text Available Clonal plants that produced by somatic embryogenesis technique is one of the best choice to produce supperior clonal cacao (Theobroma cacao L. planting materials. The somatic embryogenesis technique is a possible way for massive propagation, the outcome is true to type plants, the architecture similarity that the seedlings but there is not segregation like seedlings plants. At present mass production started of plantlets production until post-acclimatized plantlets of somatic embryogenesis cocoa was done at Indonesian Coffee and Cocoa Research Institute. Distribution system of the planting materials to whole areas in form of as up-rooted post-acclimatized plantlet. Some problems identified to reduce probability of decreasing viability of up-rooted post-acclimatized plantlets and one of them is extreme internal water deficit. This research investigate of the influece storage condition (air tight and non-air tight and box storage (mica plastic and cardboardbox. The first experiment result show, there is no significant different between mica plastic and cardboard box usage for storage of post-acclimatized cocoa pantlet. Viability of up-rooted post acclimatized cocoa plantlet influenced exactly by air tight and non-air tight storage condition. Air tight storage condition have better viability of up-rooted post acclimatised (81,58% than non-air tight storage condition (65,00%. Leaf sanasence on air tight storage condition (10,33% lower than non-air tight storage (32,58%. There is not significantly on volume storage per plantlet between 4.416 cm3 and 12.600 cm3. Relationship between fallen leaves and cocoa planlet viability follow negative linear correlation y = -1,4719x + 104,88 (R2 = 0,9772. The second experiment treatment showed that maximal storage periode of post cclimatized cocoa plantlet just until 6 days stored (97% and not significant with 3 days one. Viability of post acclimatized cocoa plantlet decreased after 6 days storage period

  11. Temporal variations of potential fecundity of southern blue whiting (Micromesistius australis australis) in the Southeast Pacific

    Science.gov (United States)

    Flores, Andrés; Wiff, Rodrigo; Díaz, Eduardo; Carvajal, Bernardita

    2017-08-01

    Fecundity is a key aspect of fish species reproductive biology because it relates directly to total egg production. Yet, despite such importance, fecundity estimates are lacking or scarce for several fish species. The gravimetric method is the most-used one to estimate fecundity by essentially scaling up the oocyte density to the ovary weight. It is a relatively simple and precise technique, but also time consuming because it requires counting all oocytes in an ovary subsample. The auto-diametric method, on the other hand, is relatively new for estimating fecundity, representing a rapid alternative, because it requires only an estimation of mean oocyte density from mean oocyte diameter. Using the extensive database available from commercial fishery and design surveys for southern blue whiting Micromesistius australis australis in the Southeast Pacific, we compared estimates of fecundity using both gravimetric and auto-diametric methods. Temporal variations in potential fecundity from the auto-diametric method were evaluated using generalised linear models considering predictors from maternal characteristics such as female size, condition factor, oocyte size, and gonadosomatic index. A global and time-invariant auto-diametric equation was evaluated using a simulation procedure based on non-parametric bootstrap. Results indicated there were not significant differences regarding fecundity estimates between the gravimetric and auto-diametric method (p > 0.05). Simulation showed the application of a global equation is unbiased and sufficiently precise to estimate time-invariant fecundity of this species. Temporal variations on fecundity were explained by maternal characteristic, revealing signals of fecundity down-regulation. We discuss how oocyte size and nutritional condition (measured as condition factor) are one of the important factors determining fecundity. We highlighted also the relevance of choosing the appropriate sampling period to conduct maturity studies

  12. Southern right whales Eubalaena australis visit the coasts of ...

    African Journals Online (AJOL)

    spamer

    Counts and photographs of right whales Eubalaena australis taken on aerial surveys of the southern coast of. South Africa between ... of 0.08–2.89 km.h-1. Theodolite tracking of undisturbed groups of right whales from Cape Columbine produced ...... right whales on the coast of Argentina (Outer Coast) between 1971 and ...

  13. Isolation of fucoxanthin and fatty acids analysis of Padina australis ...

    African Journals Online (AJOL)

    Fucoxanthin has been successfully isolated from species of Malaysian brown seaweed, namely Padina australis. The purity of the fucoxanthin is >98% as indicated by high performance liquid chromatography analysis. This seaweed also contains a considerable amount of unsaturated fatty acids. Thirteen fatty acids were ...

  14. Histomorphology of the digestive tract of Chonopeltis australis ...

    African Journals Online (AJOL)

    The morphology and histology of the digestive tract of the branchiuran crustacean, Chonopeltis australis Box shall, 1976 are described from serial sections. The foregut is differentiated into a preoral cavity, containing the mandibles and tongue, an ascending oesophagus, with an H-shaped lumen invested with longitudinal, ...

  15. Plant defense induced in in vitro propagated banana (Musa paradisiaca) plantlets by Fusarium derived elicitors.

    Science.gov (United States)

    Patel, Miral; Kothari, I L; Mohan, J S S

    2004-07-01

    Perception of microbial signal molecules is part of the strategy evolved by plants to survive attacks by potential pathogens. To gain a more complete understanding of the early signaling events involved in these responses, we used fungal components of Fusarium under in vitro condition and checked the rise in signal molecule, salicylic acid (SA), and marker enzymes in defense reactions against the pathogen. SA level increased by 21 folds in elicitor treated plantlets as compared to that of control plantlets and there was marked increase in phenylalanine ammonia-lyase(PAL), peroxidase(POX), polyphenol oxidase(PPO) along with higher total phenolic content. Present results indicated that use of fungal components had successfully induced systemic resistance in in vitro cultured banana plantlets.

  16. Use of slow-release fertilizer on the production of sweet potatoes plantlets in tray

    Directory of Open Access Journals (Sweden)

    Amarílis Beraldo Rós

    2013-12-01

    Full Text Available The production of plantlets in containers generally requires the use of fertilizers. The aim of this study was to evaluate the production of sweet potato in styrofoam trays using slow-release fertilizer. The experiment was carried out, under a screen-protected nursery, in a factorial scheme 5x5, with five doses of slow-release fertilizer NPK 19-06-10 (0, 50, 100, 150 e 200 g per 25 kg de substrate and five times of plantlets permanence in tray (14, 28, 42, 56 and 70 days after planting the cuttings. The number and dry matter of roots and leaves were evaluated. The number of roots was not influenced by fertilizer addition. In general, there is not damage to plantlets growth until the highest dose used. Therefore, the fertilizer addition increases the sweet potato plants growth and the dose of 200 g per 25 kg of substrate is responsible for the best results.

  17. Host plant development, water level and water parameters shape Phragmites australis-associated oomycete communities and determine reed pathogen dynamics in a large lake.

    Science.gov (United States)

    Wielgoss, Anna; Nechwatal, Jan; Bogs, Carolin; Mendgen, Kurt

    2009-08-01

    In a 3-year-study, we analysed the population dynamics of the reed pathogen Pythium phragmitis and other reed-associated oomycetes colonizing fresh and dried reed leaves in the littoral zone of a large lake. Oomycete communities derived from internal transcribed spacer clone libraries were clearly differentiated according to substrate and seasonal influences. In fresh leaves, diverse communities consisting of P. phragmitis and other reed-associated pathogens were generally dominant. Pythium phragmitis populations peaked in spring with the emergence of young reed shoots, and in autumn after extreme flooding events. In summer it decreased with falling water levels, changing water chemistry and rising temperatures. Another Pythium species was also highly abundant in fresh leaves throughout the year and might represent a new, as-yet uncultured reed pathogen. In dried leaves, reed pathogens were rarely detected, whereas saprophytic species occurred abundantly during all seasons. Saprophyte communities were less diverse, less temperature sensitive and independent of reed development. In general, our results provide evidence for the occurrence of highly specialized sets of reed-associated oomycetes in a natural reed ecosystem. Quantitative analyses (clone abundances and quantitative real-time PCR) revealed that the reed pathogen P. phragmitis is particularly affected by changing water levels, water chemistry and the stage of reed development.

  18. Do ploidy level and nuclear genome size and latitude of origin modify the expression of Phragmites australis traits and interaction with herbivores?

    Czech Academy of Sciences Publication Activity Database

    Meyerson, L. A.; Cronin, J. T.; Bhattarai, G. P.; Brix, H.; Lambertini, C.; Lučanová, Magdalena; Rinehart, S.; Suda, Jan; Pyšek, Petr

    2016-01-01

    Roč. 18, č. 9 (2016), s. 2531-2549 ISSN 1387-3547 R&D Projects: GA ČR(CZ) GA14-15414S Grant - others:AV ČR(CZ) AP1002 Program:Akademická prémie - Praemium Academiae Institutional support: RVO:67985939 Keywords : traits * genome size * plant invasions Subject RIV: EH - Ecology, Behaviour Impact factor: 2.473, year: 2016

  19. Observations on the colonization of a young polder area in the Netherlands with special reference to the clonal expansion of Phragmites australis

    NARCIS (Netherlands)

    Clevering, O.A.; Van der Toorn, J.

    2000-01-01

    In 1968, the polder Zuidelijk Flevoland was reclaimed in the Netherlands. Observations on species succession were made during a period of seven years in an area later known as the nature reserve Oostvaardersplassen. Directly after reclamation, a large number of plant species were recruited. Here,

  20. Internal oxygen dynamics in rhizomes of Phragmites australis and presence of methanotrophs in root biofilms in a constructed wetland for wastewater treatment

    Czech Academy of Sciences Publication Activity Database

    Faußer, A.; Dušek, Jiří; Čížková, Hana; Hoppert, M.; Walther, P.; Kazda, M.

    2013-01-01

    Roč. 51, 13-15 (2013), s. 3026-3031 ISSN 1944-3994. [3rd International Conference on Environmental Management, Engineering, Planning and Economics (CEMEPE) and SECOTOX Conference. Skiathos Island, 19.06.2011-24.06.2011] Institutional support: RVO:67179843 Keywords : Aerenchyma * Macrophyte * Constructed wetland * Internal oxygen partial pressure * Methane-oxidising bacteria Subject RIV: EH - Ecology, Behaviour Impact factor: 0.988, year: 2013

  1. Retrieving aboveground biomass of wetland Phragmites australis (common reed) using a combination of airborne discrete-return LiDAR and hyperspectral data

    Science.gov (United States)

    Luo, Shezhou; Wang, Cheng; Xi, Xiaohuan; Pan, Feifei; Qian, Mingjie; Peng, Dailiang; Nie, Sheng; Qin, Haiming; Lin, Yi

    2017-06-01

    Wetland biomass is essential for monitoring the stability and productivity of wetland ecosystems. Conventional field methods to measure or estimate wetland biomass are accurate and reliable, but expensive, time consuming and labor intensive. This research explored the potential for estimating wetland reed biomass using a combination of airborne discrete-return Light Detection and Ranging (LiDAR) and hyperspectral data. To derive the optimal predictor variables of reed biomass, a range of LiDAR and hyperspectral metrics at different spatial scales were regressed against the field-observed biomasses. The results showed that the LiDAR-derived H_p99 (99th percentile of the LiDAR height) and hyperspectral-calculated modified soil-adjusted vegetation index (MSAVI) were the best metrics for estimating reed biomass using the single regression model. Although the LiDAR data yielded a higher estimation accuracy compared to the hyperspectral data, the combination of LiDAR and hyperspectral data produced a more accurate prediction model for reed biomass (R2 = 0.648, RMSE = 167.546 g/m2, RMSEr = 20.71%) than LiDAR data alone. Thus, combining LiDAR data with hyperspectral data has a great potential for improving the accuracy of aboveground biomass estimation.

  2. Influence of tidal regime on the distribution of trace metals in a contaminated tidal freshwater marsh soil colonized with common reed (Phragmites australis)

    International Nuclear Information System (INIS)

    Teuchies, J.; Deckere, E. de; Bervoets, L.; Meynendonckx, J.; Regenmortel, S. van; Blust, R.; Meire, P.

    2008-01-01

    A historical input of trace metals into tidal marshes fringing the river Scheldt may be a cause for concern. Nevertheless, the specific physicochemical form, rather than the total concentration, determines the ecotoxicological risk of metals in the soil. In this study the effect of tidal regime on the distribution of trace metals in different compartments of the soil was investigated. As, Cd, Cu and Zn concentrations in sediment, pore water and in roots were determined along a depth profile. Total sediment metal concentrations were similar at different sites, reflecting pollution history. Pore water metal concentrations were generally higher under less flooded conditions (mean is (2.32 ± 0.08) x 10 -3 mg Cd L -1 and (1.53 ± 0.03) x 10 -3 mg Cd L -1 ). Metal concentrations associated with roots (mean is 202.47 ± 2.83 mg Cd kg -1 and 69.39 ± 0.99 mg Cd kg -1 ) were up to 10 times higher than sediment (mean is 20.48 ± 0.19 mg Cd kg -1 and 20.42 ± 0.21 mg Cd kg -1 ) metal concentrations and higher under dryer conditions. Despite high metal concentrations associated with roots, the major part of the metals in the marsh soil is still associated with the sediment as the overall biomass of roots is small compared to the sediment. - Pore water metal concentrations and metal root plaque concentration are influenced by the tidal regime

  3. Monitoring the Short-Term Response to Salt Exposure of Two Genetically Distinct Phragmites australis Clones with Different Salinity Tolerance Levels

    DEFF Research Database (Denmark)

    Achenbach, Luciana; Brix, Hans

    2014-01-01

    work was conducted at the laboratory of the Institute of Bioscience, Aarhus University, Denmark. Methods: The light-saturated photosynthetic rate (Pmax), stomata conductance (gs) and transpiration rate (E) were measured over different periods of salt exposure (15, 70 and 240 minutes) and at different...... in the 15-minute experiment. The Greeny-type also recovered after the 70-minute exposure, but not the Land-type. We conclude that the response to osmotic stress is genotype-dependent and that the salt-tolerant clone possesses very efficient signaling pathways to detect changes in the soil water potential...

  4. Ultrastructural and physiological responses of potato (Solanum tuberosum L.) plantlets to gradient saline stress

    Science.gov (United States)

    Gao, Hui-Juan; Yang, Hong-Yu; Bai, Jiang-Ping; Liang, Xin-Yue; Lou, Yan; Zhang, Jun-Lian; Wang, Di; Zhang, Jin-Lin; Niu, Shu-Qi; Chen, Ying-Long

    2015-01-01

    Salinity is one of the major abiotic stresses that impacts plant growth and reduces the productivity of field crops. Compared to field plants, test tube plantlets offer a direct and fast approach to investigate the mechanism of salt tolerance. Here we examined the ultrastructural and physiological responses of potato (Solanum tuberosum L. c.v. “Longshu No. 3”) plantlets to gradient saline stress (0, 25, 50, 100, and 200 mM NaCl) with two consequent observations (2 and 6 weeks, respectively). The results showed that, with the increase of external NaCl concentration and the duration of treatments, (1) the number of chloroplasts and cell intercellular spaces markedly decreased, (2) cell walls were thickened and even ruptured, (3) mesophyll cells and chloroplasts were gradually damaged to a complete disorganization containing more starch, (4) leaf Na and Cl contents increased while leaf K content decreased, (5) leaf proline content and the activities of catalase (CAT) and superoxide dismutase (SOD) increased significantly, and (6) leaf malondialdehyde (MDA) content increased significantly and stomatal area and chlorophyll content decline were also detected. Severe salt stress (200 mM NaCl) inhibited plantlet growth. These results indicated that potato plantlets adapt to salt stress to some extent through accumulating osmoprotectants, such as proline, increasing the activities of antioxidant enzymes, such as CAT and SOD. The outcomes of this study provide ultrastructural and physiological insights into characterizing potential damages induced by salt stress for selecting salt-tolerant potato cultivars. PMID:25628634

  5. Potato transformation and potato cyst nematode infection on potato plantlets in tissue culture

    Science.gov (United States)

    These two protocols describe the methods for generating transgenic potato plants and for evaluating potato cyst nematode (Globodera rostochiensis and G. pallida) infection on potato plantlets in tissue culture. These methods are useful tools that can be used in the study of the interactions between ...

  6. Pertumbuhan Plantlet Anggrek Cattleya sp. dengan Perlakuan Benzyl Amino Purine pada Media Dasar Pupuk Daun Modifikasi

    Directory of Open Access Journals (Sweden)

    HESTIN YUSWANTI

    2015-09-01

    Full Text Available Growth of Plantlets of Cattleya Orchid on The Fooliar Fertilizer-Based Medium added with Benzyl Amino Purine. The aim of the current research was to investigate the appropriate concentrationof plant growth regulator BAP on the growth of Cattleya plantlet. The experiment was utilized a Randomized Completely Design with five treatments and six replications. The basal medium used was modification of foliar fertilizer of Growmore (trade mark with addition of fish emulsion, Vitamin B1 and active charcoal. BAP concentration used as treatment were 0 ppm, 0.5 ppm, 1.0 ppm, 1.5 ppm and 2.0 ppm. Variables observed were height, leaf number, root number, root length, fresh weight and dry weight of plantlets. The result showed that concentration of 1 ppm BAP resulted in the highest value for plantlet height (5.67 cm, leaf number (4.67, root length (2.07 cm, fresh weight (0.36 g and dry weight (0.043 g.

  7. Ultrastructural and physiological responses of potato (Solanum tuberosum L. plantlets to gradient saline stress

    Directory of Open Access Journals (Sweden)

    Hui-Juan eGao

    2015-01-01

    Full Text Available Salinity is one of the major abiotic stresses that impacts plant growth and reduces the productivity of field crops. Compared to field plants, test tube plantlets offer a direct and fast approach to investigate the mechanism of salt tolerance. Here we examined the ultrastructural and physiological responses of potato (Solanum tuberosum L. c.v. ‘Longshu No. 3’ plantlets to gradient saline stress (0, 25, 50, 100 and 200 mM NaCl with two consequent observations (two and six weeks, respectively. The results showed that, with the increase of external NaCl concentration and the duration of treatments, (1 the number of chloroplasts and cell intercellular spaces markedly decreased, (2 cell walls were thickened and even ruptured, (3 mesophyll cells and chloroplasts were gradually damaged to a complete disorganization containing more starch, (4 leaf Na and Cl contents increased while leaf K content decreased, (5 leaf proline content and the activities of catalase (CAT and superoxide dismutase (SOD increased significantly, and (6 leaf malondialdehyde (MDA content increased significantly and stomatal area and chlorophyll content decline were also detected. Severe salt stress (200 mM NaCl inhibited plantlet growth. These results indicated that potato plantlets adapt to salt stress to some extent through accumulating osmoprotectants, such as proline, increasing the activities of antioxidant enzymes, such as CAT and SOD. The outcomes of this study provide ultrastructural and physiological insights into characterizing potential damages induced by salt stress for selecting salt-tolerant potato cultivars.

  8. EX VITRO ROOTING OF OIL PALM (Elaeis guineensis Jacq. PLANTLETS DERIVED FROM TISSUE CULTURE

    Directory of Open Access Journals (Sweden)

    Sumaryono Sumaryono

    2011-10-01

    Full Text Available Plantlets of oil palm (Elaeis guineensis Jacq. derived from so-matic embryos sometimes do not form well developed-roots. Root formation of unrooted-plantlets can be induced with aux-in during ex vitro acclimatization period to simplify the proce-dure and to reduce seedling production cost. Experiments were conducted using a completely randomized design to determine the effect of different types of auxin, i.e. indole-3-acetic acid (IAA, indole-3-butyric acid (IBA, and 1-naphthalene-acetic acid (NAA at different concentrations, i.e. 0, 2, 4, 8, and 16 mM on root development of oil palm plantlets. The plantlets used were derived from somatic embryos of MK 649 oil palm clone. The basal end of the shoots was dipped in auxin solution for 10 minutes before the shoot was cultured in a small plastic pot containing a mixed growing medium. The cultures were then placed inside a closed transparent plastic tunnel (240 cm x 100 cm x 95 cm for 12 weeks. The results showed that without auxin treatment only 15% of the shoots formed roots. Dipping in auxin solution increased significantly root frequen-cy to more than 50%. The best root formation was found on the shoots treated with 2 mM NAA by which rooting frequency was 80%. Auxin treatments also increased root quality as indi-cated by more number of primary and secondary roots. IAA, IBA, and NAA treatments at all concentrations tested increased significantly shoot height on average by 42% and shoot diame-ter by 30% compared to control treatment, but did not influ-ence root length. The best treatment for inducing roots of oil palm plantlets ex vitro was by dipping the basal end of the plant-lets in 2 mM NAA solution. The result showed that rooting of oil palm plantlets could be successfully conducted ex vitro that would eliminate sterile rooting stage thus simplify the protocol and reduce seedling production time and cost.

  9. The presence of eucalyptol in Artemisia australis validates its use in traditional Hawaiian medicine

    Institute of Scientific and Technical Information of China (English)

    David Zant; Daniel A. Gubler

    2014-01-01

    Objective: To identify the major organic compounds of Artemisia australis (A. australis), a plant used in traditional Hawaiian medicine for the treatment of asthma.Methods:The dichloromethane extract of A. australis was analyzed by gas chromatography-mass spectroscopy and major compounds were identified by a National Institute of Standards and Technology library search and confirmed by peak enhancement.Results:The major chemical components of A. australis include eucalyptol, borneol, and caryophyllene.Conclusions:The presence and biological activity of eucalyptol correlate very well with the usage of this plant in traditional Hawaiian medicine.

  10. Effects of ventilation and sucrose concentrations on the growth and plantlet anatomy of micropropagated persian walnut plants

    Science.gov (United States)

    Plantlets grown in conventional tissue culture systems usually encounter physiological and anatomical abnormalities including inability to photosynthesize, low chlorophyll content, open stomata, lack of a cuticle layer in the leaf, abnormal xylem parenchyma etc. Photoautotrophic and photomixotrophic...

  11. Effects of gamma radiation on in vitro plantlets of Alpinia purpurata

    International Nuclear Information System (INIS)

    Fereol, L.; Louis, S.; Luce, L.

    1996-01-01

    Two cultivars of Alpinia purpurata were propagated by in vitro culture to provide numerous convenient plantlets that were exposed to 0, 15, 30, or 45 Gy of gamma rays to study their radiosensitivity. The plants of the C1 and C2 clonal generations showed decreases in plant survival rate, fresh weight gain and micropropagation rate with increasing doses of gamma radiation. On the basis of survival, LD 50 was approximately 30 Gy. The 45 Gy dose drastically affects the different responses studied. It proved lethal or induced dwarf plants. Some plantlets, produced in vitro from treated plants, showed morphological abnormalities of leaves or of whole plants. This technique has potential for increasing the genetic variation in Alpinia purpurata. (author)

  12. Proteomic Analysis of Responsive Proteins Induced in Japanese Birch Plantlet Treated with Salicylic Acid

    Directory of Open Access Journals (Sweden)

    Hiromu Suzuki

    2014-07-01

    Full Text Available The present study was performed to unravel the mechanisms of systemic acquired resistance (SAR establishment and resistance signaling pathways against the canker-rot fungus (Inonotus obliquus strain IO-U1 infection in Japanese birch plantlet No.8. Modulation of protein-profile induced by salicylic acid (SA-administration was analyzed, and SA-responsive proteins were identified. In total, 5 specifically expressed, 3 significantly increased, and 3 significantly decreased protein spots were identified using liquid chromatography/tandem mass spectrometry (LC/MS/MS and the sequence tag method. These proteins were malate dehydrogenase, succinate dehydrogenase, phosphoglycerate kinase, diaminopimalate decarboxylase, arginase, chorismate mutase, cyclophilin, aminopeptidase, and unknown function proteins. These proteins are considered to be involved in SAR-establishment mechanisms in the Japanese birch plantlet No 8.

  13. Hormonal control of root development on epiphyllous plantlets of Bryophyllum (Kalanchoe) marnierianum: role of auxin and ethylene.

    Science.gov (United States)

    Kulka, Richard G

    2008-01-01

    Epiphyllous plantlets develop on leaves of Bryophyllum marnierianum when they are excised from the plant. Shortly after leaf excision, plantlet shoots develop from primordia located near the leaf margin. After the shoots have enlarged for several days, roots appear at their base. In this investigation, factors regulating plantlet root development were studied. The auxin transport inhibitor 2,3,5-triiodobenzoic acid (TIBA) abolished root formation without markedly affecting shoot growth. This suggested that auxin transport from the plantlet shoot induces root development. Excision of plantlet apical buds inhibits root development. Application of indole-3-acetic acid (IAA) in lanolin at the site of the apical buds restores root outgrowth. Naphthalene acetic acid (NAA), a synthetic auxin, reverses TIBA inhibition of plantlet root emergence on leaf explants. Both of these observations support the hypothesis that auxin, produced by the plantlet, induces root development. Exogenous ethylene causes precocious root development several days before that of a control without hormone. Ethylene treatment cannot bypass the TIBA block of root formation. Therefore, ethylene does not act downstream of auxin in root induction. However, ethylene amplifies the effects of low concentrations of NAA, which in the absence of ethylene do not induce roots. Ag(2)S(2)O(3), an ethylene blocker, and CoCl(2), an ethylene synthesis inhibitor, do not abolish plantlet root development. It is therefore unlikely that ethylene is essential for root formation. Taken together, the experiments suggest that roots develop when auxin transport from the shoot reaches a certain threshold. Ethylene may augment this effect by lowering the threshold and may come into play when the parent leaf senesces.

  14. EFEKTIVITAS PERUPUK (Phragmites karka DAN MIKROORGANISME EFEKTIF (EM DALAM PENGOLAHAN LIMBAH CAIR DOMESTIK RUMAH TANGGA

    Directory of Open Access Journals (Sweden)

    Muhammad Ricky Saputra

    2016-10-01

    The results obtained from this study is that in water reservoir the combination of EM4 and Perupuk (Phragmites karka was the best treatment in this study that have been executed. The combination of two treatments that were able to repair four of the seven water quality parameters are DO (-31.48%, BOD5 (99.57%, COD (99.87%, and TSS (92.41% from standard of environment quality in Peraturan Pemerintah (PP No.82 tahun 2001 Kelas II

  15. Influence of arbuscular mycorrhizas on the growth rate of mist-propagated olive plantlets

    OpenAIRE

    Porras Piedra, A.; Soriano Martín, M.L.; Porras Soriano, A.; Fernández Izquierdo, G.

    2005-01-01

    The aim of this work was to determine the influence of early inoculation of semi-woody olive cuttings (cv. Cornicabra), cultivated under mist propagation conditions, with arbuscular mycorrhizal fungi. A strongly positive response was seen to root inoculation with the three fungal species tested —Glomus mosseae, G. intraradices and G. claroideum— both in nursery phase plantlets, and in older plants grown in 5 L pots.

  16. Diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple plantlets during acclimatization

    OpenAIRE

    Silva,Aurilena de Aviz; Carvalho,Almy Junior Cordeiro de; Freitas,Flávia Paiva de; Pessanha,Patrícia Gomes de Oliveira; Santos,Paulo Cesar dos; Silva,Mírian Peixoto Soares da; Vasconcelos,Tábatha de Souza; Olivares,Fábio Lopes

    2016-01-01

    ABSTRACT: This study examines the effect of inoculation with diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple cv. 'Vitória' plantlets during the acclimatization period. The experiment was carried out in a greenhouse in Campos dos Goytacazes, in randomized blocks, using a 2x5x5 factorial scheme, with the factors being two types of inocula (absence or presence of a mixture of diazotrophic bacteria that contained Burkholderia sp. UENF 114111, Burkholder...

  17. EFFECTS INDUCED BY DIETHYL SULPHATE ON SOME CYTOGENETICAL PARAMETERS AND LENGTH GROWTH OF HEMP PLANTLETS

    Directory of Open Access Journals (Sweden)

    Elena Truta

    2007-08-01

    Full Text Available The hemp seeds were treated with diethyl sulphate, in four concentrations (0.1%, 0.25%, 0.5%, and 1% and in two variants of alkylant exposure (3 and 6 hours. The length growth of plantlets, mitotic index and frequency of chromosomal aberrations were the analyzed parameters. Significant modifications were obtained at the level of parameters in variants treated with DES, comparatively with control.

  18. Comparative physiological and proteomic analyses of poplar (Populus yunnanensis plantlets exposed to high temperature and drought.

    Directory of Open Access Journals (Sweden)

    Xiong Li

    Full Text Available Plantlets of Populus yunnanensis Dode were examined in a greenhouse for 48 h to analyze their physiological and proteomic responses to sustained heat, drought, and combined heat and drought. Compared with the application of a single stress, simultaneous treatment with both stresses damaged the plantlets more heavily. The plantlets experienced two apparent response stages under sustained heat and drought. During the first stage, malondialdehyde and reactive oxygen species (ROS contents were induced by heat, but many protective substances, including antioxidant enzymes, proline, abscisic acid (ABA, dehydrin, and small heat shock proteins (sHSPs, were also stimulated. The plants thus actively defended themselves against stress and exhibited few pathological morphological features, most likely because a new cellular homeostasis was established through the collaborative operation of physiological and proteomic responses. During the second stage, ROS homeostasis was overwhelmed by substantial ROS production and a sharp decline in antioxidant enzyme activities, while the synthesis of some protective elements, such as proline and ABA, was suppressed. As a result, photosynthetic levels in P. yunnanensis decreased sharply and buds began to die, despite continued accumulation of sHSPs and dehydrin. This study supplies important information about the effects of extreme abiotic environments on woody plants.

  19. Signal mediators at induction of heat resistance of wheat plantlets by short-term heating

    Directory of Open Access Journals (Sweden)

    Yu. V. Karpets

    2015-12-01

    Full Text Available The effects of functional interplay of calcium ions, reactive oxygen species (ROS and nitric oxide (NO in the cells of wheat plantlets roots (Triticum aestivum L. at the induction of their heat resistance by a short-term influence of hyperthermia (heating at the temperature of 42 °С during 1 minute have been investigated. The transitional increase of NO and H2O2 content, invoked by heating, was suppressed by the treatment of plantlets with the antagonists of calcium EGTA (chelator of exocellular calcium, lanthanum chloride (blocker of calcium channels of various types and neomycin (inhibitor of phosphatidylinositol-dependent phospholipase C. The rise of hydrogen peroxide content, caused by hardening, was partially suppressed by the action of inhibitors of nitrate reductase (sodium wolframate and NO-synthase (NG-nitro-L-arginine methyl ester – L-NAME, and the increasing of nitric oxide content was suppressed by the treatment of plants with the antioxidant ionol and with the scavenger of hydrogen peroxide (dimethylthiourea. These compounds and antagonists of calcium also partially removed the effect of the rise of plantlets’ heat resistance, invoked by hardening heating. The conclusion on calcium’s role in the activation of enzymatic systems, generating reactive oxygen species and nitric oxide, and on the functional interplay of these signal mediators at the induction of heat resistance of plantlets by hardening heating is made.

  20. Diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple plantlets during acclimatization

    Directory of Open Access Journals (Sweden)

    Aurilena de Aviz Silva

    Full Text Available ABSTRACT: This study examines the effect of inoculation with diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple cv. 'Vitória' plantlets during the acclimatization period. The experiment was carried out in a greenhouse in Campos dos Goytacazes, in randomized blocks, using a 2x5x5 factorial scheme, with the factors being two types of inocula (absence or presence of a mixture of diazotrophic bacteria that contained Burkholderia sp. UENF 114111, Burkholderia silvatlantica UENF 11711, and Herbaspirillum seropedicae HRC 54, five levels of urea (0, 2, 5, 7, and 10g L-1, and five evaluation periods (30, 60, 90, 120, and 150 days following the planting of plantlets in trays, and with four replications of each treatment. An increase in the dried weight of shoots and roots of micropropagated pineapple cv. 'Vitória' plants depended on inoculation with diazotrophic bacteria, and plantlets acclimatization could be reduced by up to 57 days, depending on the concentration of urea fertilizer.

  1. SPECTRAL QUALITY AFFECTS MORPHOGENESIS ON ANTHURIUM PLANTLET DURING IN VITRO CULTURE

    Directory of Open Access Journals (Sweden)

    Kurniawan Budiarto

    2010-10-01

    Full Text Available This paper elucidates the effects of LEDs spectral on callus induction, proliferation and shoot development of anthurium plantlet derived from leaf explants. The research was conducted at the Ornamental Research Station, Fukuyama, Japan from January to August 2008. Three experimental series were designed to determine the effects of LED-based spectral compositions i.e. 100% red, 75% red + 25% blue, 50% red + 50% blue, 25% red + 75% blue and 100% blue LEDs on morphogenetic process of callus formation derived from leaf explants up to plantlet formation on two anthurium cultivars, Violeta and Pink Lady. The results showed no differences among cultivars tested but interaction of factors studied were found in all parameters observed. LEDs spectral gave significant influence on the morphogenetic processes from callus induction to complete plantlet formation. Progressive initial callus was promoted with the decrease of blue LEDs portion. Conversely, to proliferate globose to torpedo callus formation, more blue light was required than red LEDs. During shoot induction and formation, hastened shoot initiation and number of shoots were achieved in higher blue LEDs portions, but not in root formations.

  2. Key KdSOC1 gene expression profiles during plantlet morphogenesis under hormone, photoperiod, and drought treatments.

    Science.gov (United States)

    Liu, C; Zhu, C; Zeng, H M

    2016-02-11

    Kalanchoe daigremontiana utilizes plantlet formation between its zigzag leaf margins as its method of asexual reproduction. In this study, K. daigremontiana SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (KdSOC1), a key intermediate in the transition from vegetative to asexual growth, was cloned. Furthermore, its expression profiles during plantlet formation under different environmental and hormone induction conditions were analyzed. The full-KdSOC1 cDNA sequence length was 1410 bp with 70% shared homology with Carya cathayensis SOC1. The conserved domain search of KdSOC1 showed the absence of I and C domains, which might indicate novel biological functions in K. daigremontiana. The full-KdSOC1 promoter sequence was 1401 bp long and contained multiple-hormone-responsive cis-acting elements. Hormone induction assays showed that gibberellins and salicylic acid mainly regulated KdSOC1 expression. The swift change from low to high KdSOC1 expression levels during long-day induction was accompanied by the rapid emergence of plantlets. Drought stress stimulated KdSOC1 expression in leaves both with and without plantlet formation. Together, the results suggested that KdSOC1 was closely involved in environmental stimulation signal perception and the transduction of K. daigremontiana plantlet formation. Therefore, future identification of KdSOC1 functions might reveal key information that will help elucidate the transition network between embryogenesis and organogenesis during plantlet formation.

  3. Effect of Plantago australis leaves on different gastric ulcer models

    Directory of Open Access Journals (Sweden)

    M.E. Bürger

    Full Text Available The anti-ulcerogenic effect of the crude ethanolic extract (CEE of Plantago australis leaves was tested against ethanol-, indomethacin-, and cold restrain-induced stress ulcers. The CEE (500 and 1000 mg/kg reduced the lesion index (LI and the ulcer index in ethanol-induced ulcers, and the dose of 1000 mg/kg increased the amount of mucous. The highest dose of the CEE reduced the LI of cold restraint-induced stress ulcers when compared to the control group. The indomethacin-induced ulcers were not affected by this extract.

  4. Gamma irradiation as activator of antioxidant activity and essential oil contents in lavender (Lavandula multifida) plantlets

    International Nuclear Information System (INIS)

    El-Naggar, H.A.; Atallah, R.K.; Aly, A.A.; Maraei, R.W.

    2012-01-01

    This study was conducted to evaluate the stimulation effect of γ-irradiation on the chemical composition of essential oils, total phenolic compounds, flavonoid contents and antioxidant activities in lavender plantlets (Lavandula multifida) at three multiplication stages. Lavender plantlets were irradiated using different γ- irradiation dose levels (0.0, 5, 15, 30, 45, 60 and 75 Gy). After irradiation; plantlets were sectioned to start the multiplication stage (three subcultures). Increasing irradiation dose levels at multiplication stages significantly increased the total phenolic content and reached to the maximum increment at the dose level of 75 Gy (26.88 g/100 g DW) in zero time stage in comparison with the untreated plantlets (7.250 g/100 g DW). The highest content of flavonoids (21.50 g/100 g DW) was detected at dose level of 75 Gy at zero time stage (M0). The highest applied irradiation dose of 75 Gy gave the highest reducing power activity compared with control at zero time stage (M0). Scavenging activity by DPPH was increased gradually by increasing irradiation dose levels in all multiplication stages until the high dose of 75 Gy which gave the maximum scavenging activity (91.05%) in zero time stage. Also, there was a significant increase in antioxidant activity on linoleic acid system with increasing the dose of γ-irradiation level. The application of γ-irradiation at dose level of 15 Gy and M3 stage produced the highest value of essential oil content (0.12%), followed by 5 Gy treatments (0.082%). The most increased volatile oil compounds by γ-irradiation were; limonene which increased from 4.87% to 5.37% at 0.0 and 5 Gy, respectively and linalool increased from 86.07% to 91.5% at 0.0 and 15 Gy respectively. The present study suggests that γ-irradiation led to increase antioxidant activities of lavender plantlets by increasing the availability of free polyphenolic compounds and also the content of volatile oil. This shows that lavender plants may be

  5. Warm gas towards young stellar objects in Corona Australis

    DEFF Research Database (Denmark)

    Lindberg, Johan; Jørgensen, Jes Kristian; D. Green, Joel

    2014-01-01

    The effects of external irradiation on the chemistry and physics in the protostellar envelope around low-mass young stellar objects are poorly understood. The Corona Australis star-forming region contains the R CrA dark cloud, comprising several low-mass protostellar cores irradiated by an interm......The effects of external irradiation on the chemistry and physics in the protostellar envelope around low-mass young stellar objects are poorly understood. The Corona Australis star-forming region contains the R CrA dark cloud, comprising several low-mass protostellar cores irradiated...... by an intermediate-mass young star. We study the effects on the warm gas and dust in a group of low-mass young stellar objects from the irradiation by the young luminous Herbig Be star R CrA. Herschel/PACS far-infrared datacubes of two low-mass star-forming regions in the R CrA dark cloud are presented...... Be star R CrA. Our results show that a nearby luminous star does not increase the molecular excitation temperatures in the warm gas around a young stellar object (YSO). However, the emission from photodissociation products of H2O, such as OH and O, is enhanced in the warm gas associated...

  6. The presence of eucalyptol in Artemisia australis validates its use in traditional Hawaiian medicine

    Institute of Scientific and Technical Information of China (English)

    David; Zant; Daniel; A.Gubler

    2014-01-01

    Objective:To identify the major organic compounds of Artemisia austeralis(A.australis),a plant used in traditional Hawaiian medicine for the treatment of asthma.Methods:The dichloromethane extract of A.australis was analyzed by gas chromatography—mass spectroscopy and major compounds were identified by a National Institute of Standards and Technology library search and confirmed by peak enhancement Results:The major chemical components of A.australis include eucalyptol.borneol,and caryophyllene.Conclusions:The presence and biological activity of eucalyptol correlate very well with the usage of this plant in traditional Hawaiian medicine.

  7. CO2 and CH4 fluxes in a Spartina salt marsh and brackish Phragmites marsh in Massachusetts

    Science.gov (United States)

    Tang, J.; Wang, F.; Kroeger, K. D.; Gonneea, M. E.

    2017-12-01

    Coastal salt marshes play an important role in global and regional carbon cycling. Tidally restricted marshes reduce salinity and provide a habitat suitable for Phragmites invasion. We measured greenhouse gas (GHG) emissions (CO2 and CH4) continuously with the eddy covariance method and biweekly with the static chamber method in a Spartina salt marsh and a Phragmites marsh on Cape Cod, Massachusetts, USA. We did not find significant difference in CO2 fluxes between the two sites, but the CH4 fluxes were much higher in the Phragmites site than the Spartina marsh. Temporally, tidal cycles influence the CO2 and CH4 fluxes in both sites. We found that the salt marsh was a significant carbon sink when CO2 and CH4 fluxes were combined. Restoring tidally restricted marshes will significantly reduce CH4 emissions and provide a strong ecosystem carbon service.

  8. Modification of the 137Cs, 90Sr, and 60Co transfer to wheat plantlets by NH4+ fertilizers.

    Science.gov (United States)

    Guillén, J; Muñoz-Muñoz, G; Baeza, A; Salas, A; Mocanu, N

    2017-03-01

    Inorganic fertilizers are used as agricultural countermeasures intended to inhibit the soil to plant transfer of radionuclides after a radioactive fallout. Two NH 4 + fertilizers, diammonium phosphate (DAP) and NPK, were applied to soil contaminated with a mixture of radionuclides to analyze whether they modify the transfer of 137 Cs, 90 Sr, and 60 Co and stable elements (K, Na, Ca, and Mg) to wheat plantlets grown under controlled laboratory conditions. DAP introduced NH 4 + in the soil, which can increase 137 Cs transfer, while NPK also introduced K + , which can decrease it. The application of DAP increased the accumulation of 137 Cs in wheat plantlets with increasing application rate, so did the 137 Cs/K in plantlets. Regarding the NPK application, the 137 Cs increased in all treatments, but at maximum rate, the available K introduced by the fertilizer was probably able to partially satisfy the nutritional requirements of the wheat plantlet and the 137 Cs decreased relative to the recommended rate. The 137 Cs/K ratio in plantlet decreased with increasing NPK rates. The transfer of 90 Sr increased with increasing DAP rate and only at the maximum NPK rate. The 60 Co transfer only increased at the maximum application rates for DAP and NPK. These modifications should be considered when using these fertilizers as agricultural countermeasures.

  9. Australis: AMS for ultra sensitive trace element and isotopic studies

    Energy Technology Data Exchange (ETDEWEB)

    Sie, S H; Suter, G F [Commonwealth Scientific and Industrial Research Organisation (CSIRO), North Ryde, NSW (Australia). Div. of Exploration Geoscience

    1994-12-31

    The accelerator mass spectrometer (AMS) at the CSIRO HIAF laboratory is being upgraded to enable in-situ measurements of ultratraces and isotopic-ratios in mineralogical applications. The upgraded system will include a microbeam Cs ion source which is designed to produce better than 50 micrometre diameter Cs beam to enable analyses of monomineralic grains. The Cs primary beam will be mass analysed in order to minimize contamination of the sample. The detection system will be upgraded to enable analyses of elements up to U, at 2 MV terminal voltage for charge states 4 and 5. The system will be known as AUSTRALIS: A.M.S. for Ultra Sensitive TRAce eLement and Isotopic Studies. An overview of the system and the anticipated applications in minerals exploration and mining research are presented. 4 refs., 1 fig.

  10. Australis: AMS for ultra sensitive trace element and isotopic studies

    Energy Technology Data Exchange (ETDEWEB)

    Sie, S.H.; Suter, G.F. [Commonwealth Scientific and Industrial Research Organisation (CSIRO), North Ryde, NSW (Australia). Div. of Exploration Geoscience

    1993-12-31

    The accelerator mass spectrometer (AMS) at the CSIRO HIAF laboratory is being upgraded to enable in-situ measurements of ultratraces and isotopic-ratios in mineralogical applications. The upgraded system will include a microbeam Cs ion source which is designed to produce better than 50 micrometre diameter Cs beam to enable analyses of monomineralic grains. The Cs primary beam will be mass analysed in order to minimize contamination of the sample. The detection system will be upgraded to enable analyses of elements up to U, at 2 MV terminal voltage for charge states 4 and 5. The system will be known as AUSTRALIS: A.M.S. for Ultra Sensitive TRAce eLement and Isotopic Studies. An overview of the system and the anticipated applications in minerals exploration and mining research are presented. 4 refs., 1 fig.

  11. Australis: AMS for ultra sensitive trace element and isotopic studies

    International Nuclear Information System (INIS)

    Sie, S.H.; Suter, G.F.

    1993-01-01

    The accelerator mass spectrometer (AMS) at the CSIRO HIAF laboratory is being upgraded to enable in-situ measurements of ultratraces and isotopic-ratios in mineralogical applications. The upgraded system will include a microbeam Cs ion source which is designed to produce better than 50 micrometre diameter Cs beam to enable analyses of monomineralic grains. The Cs primary beam will be mass analysed in order to minimize contamination of the sample. The detection system will be upgraded to enable analyses of elements up to U, at 2 MV terminal voltage for charge states 4 and 5. The system will be known as AUSTRALIS: A.M.S. for Ultra Sensitive TRAce eLement and Isotopic Studies. An overview of the system and the anticipated applications in minerals exploration and mining research are presented. 4 refs., 1 fig

  12. Incorporation of 2H-labelled cadaverines into the quinolizidine alkaloids in Baptisia australis

    International Nuclear Information System (INIS)

    Robins, D.J.; Sheldrake, G.N.

    1988-01-01

    The incorporation of 2 H-labelled cadaverines into the quinolizidine alkaloids, sparteine and N-methylcytisine, in Baptisia australis has been studied in order to gain more information about the formation of these alkaloids. (author)

  13. Cosmeceuticals based on Rhealba(®) Oat plantlet extract for the treatment of acne vulgaris.

    Science.gov (United States)

    Fabbrocini, G; Saint Aroman, M

    2014-12-01

    Recent evidence suggests that acne vulgaris begins as an inflammation in and around the sebaceous gland and alterations in the lipid content of sebum, which drive hyperproliferation and increased desquamation of keratinocytes within sebaceous follicles. This prevents sebum drainage, causing the formation of microcomedones, which spontaneously regress or become acne lesions when the pilosebaceous unit is further blocked by the accumulation of corneocytes. These conditions are favourable for the proliferation of Propionibacterium acnes, which further aggravates acne by enhancing abnormal desquamation, sebum production and inflammation. Also, skin fragility due to inflammation or irritation by anti-comedogenic agents can worsen the situation. Rhealba(®) Oat plantlet extract (Pierre Fabre Dermo Cosmetique) soothes and restores fragile skin in acne by reducing inflammation and inhibits bacterial adhesion of Propionibacterium acnes. Cosmeceuticals combining Rhealba(®) Oat plantlet extract and hydro-compensating actives, which are available with or without anti-comedogenic hydroxy acids, provide a balanced, multifaceted approach for acne patients. © 2014 European Academy of Dermatology and Venereology.

  14. Germination and plantlet regeneration of encapsulated microshoots of aromatic rice (Oryza sativa L. Cv. MRQ 74).

    Science.gov (United States)

    Taha, Rosna Mat; Saleh, Azani; Mahmad, Noraini; Hasbullah, Nor Azlina; Mohajer, Sadegh

    2012-01-01

    Plant tissues such as somatic embryos, apical shoot tips, axillary shoot buds, embryogenic calli, and protocom-like bodies are potential micropropagules that have been considered for creating synthetic seeds. In the present study, 3-5 mm microshoots of Oryza sativa L. Cv. MRQ 74 were used as explant sources for obtaining synthetic seeds. Microshoots were induced from stem explants on Murashige and Skoog (MS) medium supplemented with 1.5 mg/L benzylaminopurine (BAP). They were encapsulated in 3% (w/v) sodium alginate, 3% sucrose, 0.1 mg/L BAP, and 0.1 mg/L α-Naphthalene acetic acid (NAA). Germination and plantlet regeneration of the encapsulated seeds were tested by culturing them on various germination media. The effect of storage period (15-30 days) was also investigated. The maximum germination and plantlet regeneration (100.0%) were recorded on MS media containing 3% sucrose and 0.8% agar with and without 0.1 mg/L BAP. However, a low germination rate (6.67%) was obtained using top soil as a sowing substrate. The germination rate of the encapsulated microshoots decreased from 93.33% to 3.33% after 30 days of storage at 4°C in the dark. Therefore, further research is being done to improve the germination rate of the synthetic seeds.

  15. Germination and Plantlet Regeneration of Encapsulated Microshoots of Aromatic Rice (Oryza sativa L. Cv. MRQ 74

    Directory of Open Access Journals (Sweden)

    Rosna Mat Taha

    2012-01-01

    Full Text Available Plant tissues such as somatic embryos, apical shoot tips, axillary shoot buds, embryogenic calli, and protocom-like bodies are potential micropropagules that have been considered for creating synthetic seeds. In the present study, 3–5 mm microshoots of Oryza sativa L. Cv. MRQ 74 were used as explant sources for obtaining synthetic seeds. Microshoots were induced from stem explants on Murashige and Skoog (MS medium supplemented with 1.5 mg/L benzylaminopurine (BAP. They were encapsulated in 3% (w/v sodium alginate, 3% sucrose, 0.1 mg/L BAP, and 0.1 mg/L α-Naphthalene acetic acid (NAA. Germination and plantlet regeneration of the encapsulated seeds were tested by culturing them on various germination media. The effect of storage period (15–30 days was also investigated. The maximum germination and plantlet regeneration (100.0% were recorded on MS media containing 3% sucrose and 0.8% agar with and without 0.1 mg/L BAP. However, a low germination rate (6.67% was obtained using top soil as a sowing substrate. The germination rate of the encapsulated microshoots decreased from 93.33% to 3.33% after 30 days of storage at 4°C in the dark. Therefore, further research is being done to improve the germination rate of the synthetic seeds.

  16. Salt stress encourages proline accumulation by regulating proline biosynthesis and degradation in Jerusalem artichoke plantlets.

    Science.gov (United States)

    Huang, Zengrong; Zhao, Long; Chen, Dandan; Liang, Mingxiang; Liu, Zhaopu; Shao, Hongbo; Long, Xiaohua

    2013-01-01

    Proline accumulation is an important mechanism for osmotic regulation under salt stress. In this study, we evaluated proline accumulation profiles in roots, stems and leaves of Jerusalem artichoke (Helianthus tuberosus L.) plantlets under NaCl stress. We also examined HtP5CS, HtOAT and HtPDH enzyme activities and gene expression patterns of putative HtP5CS1, HtP5CS2, HtOAT, HtPDH1, and HtPDH2 genes. The objective of our study was to characterize the proline regulation mechanisms of Jerusalem artichoke, a moderately salt tolerant species, under NaCl stress. Jerusalem artichoke plantlets were observed to accumulate proline in roots, stems and leaves during salt stress. HtP5CS enzyme activities were increased under NaCl stress, while HtOAT and HtPDH activities generally repressed. Transcript levels of HtP5CS2 increased while transcript levels of HtOAT, HtPDH1 and HtPDH2 generally decreased in response to NaCl stress. Our results supports that for Jerusalem artichoke, proline synthesis under salt stress is mainly through the Glu pathway, and HtP5CS2 is predominant in this process while HtOAT plays a less important role. Both HtPDH genes may function in proline degradation.

  17. Salt stress encourages proline accumulation by regulating proline biosynthesis and degradation in Jerusalem artichoke plantlets.

    Directory of Open Access Journals (Sweden)

    Zengrong Huang

    Full Text Available Proline accumulation is an important mechanism for osmotic regulation under salt stress. In this study, we evaluated proline accumulation profiles in roots, stems and leaves of Jerusalem artichoke (Helianthus tuberosus L. plantlets under NaCl stress. We also examined HtP5CS, HtOAT and HtPDH enzyme activities and gene expression patterns of putative HtP5CS1, HtP5CS2, HtOAT, HtPDH1, and HtPDH2 genes. The objective of our study was to characterize the proline regulation mechanisms of Jerusalem artichoke, a moderately salt tolerant species, under NaCl stress. Jerusalem artichoke plantlets were observed to accumulate proline in roots, stems and leaves during salt stress. HtP5CS enzyme activities were increased under NaCl stress, while HtOAT and HtPDH activities generally repressed. Transcript levels of HtP5CS2 increased while transcript levels of HtOAT, HtPDH1 and HtPDH2 generally decreased in response to NaCl stress. Our results supports that for Jerusalem artichoke, proline synthesis under salt stress is mainly through the Glu pathway, and HtP5CS2 is predominant in this process while HtOAT plays a less important role. Both HtPDH genes may function in proline degradation.

  18. Procedure for identifying and prevent risks in in vitro sugarcane plantlets marketing

    Directory of Open Access Journals (Sweden)

    Ana Rosa Hernández Freire

    2014-10-01

    Full Text Available Seed Banks and agricultural farms benefit greatly from plant in vitro Culture techniques, for their rapid propagation processes and the production of desease-free plantlets. However, the production and commercialization process can fail and it is not as successful l as it was supposed to. The objective of this study is to identify potential failure modes at the acclimatiztion stage of plantlets to ex vitro conditions at Estación Territorial de Investigaciones de la Caña de Azúcar de Villa Clara (ETICA. A methodology of Failure Mode and Effects Analysis (FMEA was applied. A value of 80 or higher was determined to be the Risk Priority Number (RPN and the more relevant failure modes were defined a t the acclimatization and commercialization stages and ground rules and assumptions were established in order to prevent and control failure modes of the process. The study and implementation of the methodology to other processes at ETICA is strongly recommended.

  19. Comparative proteomic analysis of off-type and normal phenotype somatic plantlets derived from somatic embryos of Feijoa (Acca sellowiana (O. Berg) Burret).

    Science.gov (United States)

    Fraga, Hugo Pacheco de Freitas; Agapito-Tenfen, Sarah Zanon; Caprestano, Clarissa Alves; Nodari, Rubens Onofre; Guerra, Miguel Pedro

    2013-09-01

    Morphological disorders in a relevant portion of emerged somatic embryos have been a limiting factor in the true-to-type plantlet formation in Acca sellowiana. In this sense, the present study undertook a comparison between normal phenotype and off-type somatic plantlets protein profiles by means of the 2-D DIGE proteomics approach. Off-type and normal phenotype somatic plantlets obtained at 10 and 20 days conversion were evaluated. Results indicated 12 exclusive spots between normal and off-type plantlets at 10 days conversion, and 17 exclusive spots at 20 days conversion. Also at 20 days conversion, 4 spots were differentially expressed, up- or down-regulated. Two proteins related to carbohydrate metabolism were only expressed in off-types at 10 days conversion, suggesting a more active respiratory pathway. A vicilin-like storage protein was only found in off-types at 20 days conversion, indicating that plantlets may present an abnormality in the mobilization of storage compounds, causing reduced vigor in the development of derived plantlets. The presence of heat shock proteins were only observed during formation of normal phenotype somatic plantlets, indicating that these proteins may be involved in normal morphogenesis of plantlets formed. These new findings shed light on possible genetic or epigenetic mechanisms governing A. sellowiana morphogenesis. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  20. Phosphorus storage and mobilization in coastal Phragmites wetlands: Influence of local-scale hydrodynamics

    Science.gov (United States)

    Karstens, Svenja; Buczko, Uwe; Glatzel, Stephan

    2016-04-01

    Coastal Phragmites wetlands are at the interface between terrestrial and aquatic ecosystems and are of paramount importance for nutrient regulation. They can act both as sinks and sources for phosphorus, depending on environmental conditions, sediment properties as well as on antecedent nutrient loading and sorption capacity of the sediments. The Darss-Zingst Bodden Chain is a shallow lagoon system at the German Baltic Sea coast with a long eutrophication history. It is lined almost at its entire length by reed wetlands. In order to elucidate under which conditions these wetlands act as sources or sinks for phosphorus, in-situ data of chemo-physical characteristics of water and sediment samples were combined with hydrodynamic measurements and laboratory experiments. Small-scale basin structures within the wetland serve as sinks for fine-grained particles rich in phosphorus, iron, manganese and organic matter. Without turbulent mixing the bottom water and the sediment surface lack replenishment of oxygen. During stagnant periods with low water level, low turbulence and thus low-oxygen conditions phosphorus from the sediments is released. But the sediments are capable of becoming sinks again once oxygen is resupplied. A thin oxic sediment surface layer rich in iron and manganese adsorbs phosphorus quickly. We demonstrate that sediments in coastal Phragmites wetlands can serve both as sources and sinks of soluble reactive phosphorus on a very short time-scale, depending on local-scale hydrodynamics and the state of the oxic-anoxic sediment interface.

  1. Asymbiotic germination, seedling development and plantlet propagation of Encyclia aff. oncidioides - an endangered orchid

    Directory of Open Access Journals (Sweden)

    Ewa Łojkowska

    2011-01-01

    Full Text Available In order to estimate the best germination conditions of Encyclia aff. oncidioides seeds, five different media (Fast, Knudson C modified by Vajrabhaya, Murashige and Skoog, PB2 and modified Vacin and Went with different concentrations of plant growth regulators such as benzyladenine (BA, naphthaleneacetic acid (NAA and gibberellic acid (GA3 were tested. No beneficial effect was observed when BA and NAA were applied to the germination medium and GA3 inhibited germination. The effect of light, activated charcoal, coconut water and casein hydrolysate on seed germination was also studied. The growth rate of seedlings on three different media supplemented with activated charcoal and plant growth regulators was checked. The applied plant growth regulators had no beneficial effect on the further growth of seedlings. Fast and PB2 media with 0.2% activated charcoal proved to be the best for E. aff. oncidioides seed germination, seedling development and plantlet propagation.

  2. THE EFFICIENCY OF SHOOT AND PLANTLET FORMATION OF Cephaelis ipecacuanha AFTER THREE SUBCULTURES IN VITRO

    Directory of Open Access Journals (Sweden)

    Osmar Alves Lameira

    1994-12-01

    Full Text Available Multiple adventitious shoot formed from internodal segments of Cephaelis ipecacuanha cultured 25 days on Gamborg basal medium (GAMBORG et al., 1968 supplemented with 6.66mM 6-benzylaminopurine there was a maximum of nine shoots per segment and an average of five shoots per segment formed. The presence of gibberellic acid in the subculture media promoted shoot elongation in all treatments. The shoots attained 3cm in height and rooting of 100% after 35 days of culturing upon Murashige and Skoog's basal medium (MS, added with 4.92mM indole-3-butyric acid, 0.87m gibberellic acid and 0.1% activated charcoal. Further growth was accelerated after the transfer to 1/2 MS without growth regulators. Rooted plantlets transferred to potting soil could be successfully established.

  3. Molecular and biochemical characterization in Rauvolfia tetraphylla plantlets grown from synthetic seeds following in vitro cold storage.

    Science.gov (United States)

    Faisal, Mohammad; Alatar, Abdularhaman A; Hegazy, Ahmad K

    2013-01-01

    Synseed technology is one of the most important applications of plant biotechnology for in vitro conservation and regeneration of medicinal and aromatic plants. In the present investigation, synseeds of Rauvolfia tetraphylla were produced using in vitro-proliferated shoots upon complexation of 3 % sodium alginate and 100 mM CaCl(2). The encapsulated buds were stored at 4, 8, 12, and 16 °C and high conversion was observed in synseeds stored at 4 °C for 4 weeks. The effect of different medium strength on in vitro conversion response of synseed was evaluated and the maximum conversion (80.6 %) into plantlets was recorded on half-strength woody plant medium supplemented with 7.5 μM 6-benzyladenine and 2.5 μM α-naphthalene acetic acid after 8 weeks of culture. Plantlets with well-developed shoot and roots were hardened and successfully transplanted in field condition. After 4 weeks of transfer to ex vitro conditions, the performance of synseed-derived plantlets was evaluated on the basis of some physiological and biochemical parameters and compared with the in vivo-grown plants. Short-term storage of synthetic seeds at low temperature had no negative impact on physiological and biochemical profile of the plants that survived the storage process. Furthermore, clonal fidelity of synseed-derived plantlets was also assessed and compared with mother plant using rapid amplified polymorphic DNA and inter-simple sequence repeats analysis. No changes in molecular profiles were found among the regenerated plantlets and comparable to mother plant, which confirm the genetic stability among clones. This synseed protocol could be useful for in vitro clonal multiplication, conservation, and short-term storage and exchange of germplasm of this antihypertensive drug-producing plant.

  4. Submillimeter Spectroscopy of the R Coronae Australis Molecular Cloud Region

    Science.gov (United States)

    Dunn, Marina Madeline; Walker, Christopher K.; Pat, Terrance; Sirsi, Siddhartha; Swift, Brandon J.; Peters, William L.

    2018-01-01

    The Interstellar Medium is comprised of large amounts of gas and dust which coalesce to form stars. Observing in the Terahertz regime of the electromagnetic spectrum, approximately 0.3 -300 microns, allows astronomers to study the ISM in unprecedented detail. Using the high spectral resolution imaging system of the SuperCam receiver, a 64-pixel array previously installed on the Submillimeter Telescope on Mt. Graham, AZ, we have begun a 500 square degree survey of the galactic plane. This instrument was designed to do a complete survey of the Milky Way from the ground, with the main focus being to observe two specific transitions of the carbon monoxide molecule, 12CO(3-2) and 13CO(3-2), at 345 GHz. In this work, we present results from these observations for the R Coronae Australis (R Cr A) complex, a region in the southern hemisphere of the sky, using spectroscopic data from a portion of the survey to gain better insight into the life cycle of the ISM. The majority of stars being formed here are similar to the stellar class of the Sun, making it an excellent area of observing interest. Using these results, we attempt to better ascertain the large-scale structure and kinematics inside of the molecular cloud.

  5. Activity of male pheromone of Melanesian rhinoceros beetle Scapanes australis.

    Science.gov (United States)

    Rochat, Didier; Morin, Jean-Paul; Kakul, Titus; Beaudoin-Ollivier, Laurence; Prior, Robert; Renou, Michel; Malosse, Isabelle; Stathers, Tanya; Embupa, Sebastian; Laup, Samson

    2002-03-01

    Laboratory and field investigations were carried out to investigate the nature and role of the male pheromone emitted by the Dynast beetle Scapanes australis and to develop a mass trapping technique against this major coconut pest in Papua New Guinea. We report the biological data obtained from natural and synthetic pheromone, previously described as an 84:12:4 (w/w) mixture of 2-butanol (1), 3-hydoxy-2-butanone (2), and 2,3-butanediol (3). EAG recordings from natural and synthetic pheromone and a pitfall olfactometer were poorly informative. In contrast, extensive field trapping trials with various synthetic pheromone mixtures and doses showed that 1 and 2 (formulated in polyethylene sachets in 90:5 v/v ratio) were necessary and sufficient for optimum long-range attraction. Beetles were captured in traps baited with racemic 1 plus 2, with or without a stereoisomer mixture of 3 (2.5- to 2500-mg/day doses). Plant pieces, either sugarcane or coconut, enhanced captures by the synthetic pheromone, which was active alone. Traps with the pheromone caught both sexes in a 3:2 female-male ratio. A pheromone-based mass trapping led to the capture of 2173 beetles in 14 traps surrounding 40 ha of a cocoa-coconut plantation. The captures followed a log-linear decrease during the 125-week trapping program. The role of the male pheromone and its potential for crop protection are discussed.

  6. Comparative Hepatoprotective Activity of Ethanolic Extracts of Cuscuta australis against Acetaminophen Intoxication in Wistar Rats.

    Science.gov (United States)

    Folarin, Rachael O; Omirinde, Jamiu O; Bejide, Ronald; Isola, Tajudeen O; Usende, Levi I; Basiru, Afisu

    2014-01-01

    This study investigates the comparative hepatoprotective activity of crude ethanol extracts of Cuscuta australis against acetaminophen (APAP) intoxication. Thirty-six rats were randomly divided into six groups of 6 replicates: Group 1 which served as control received water. Group 2 was orally administered 835 mg/kg body wt. of paracetamol on day 8. Groups 3 and 4 were orally administered ethanolic extracts of the seed of Cuscuta australis in doses of 125 mg/kg and 250 mg/kg, respectively, for 7 days and then intoxicated as in Group 2 on the 8th day. Groups 5 and 6 received similar oral doses of Cuscuta australis stem extracts for 7 days and then intoxicated as in Groups 3 and 4. Group 2 rats showed severe periportal hepatic necrosis, significantly elevated serum hepatic injury markers, markedly increased lipid peroxidation, and decreased hepatic antioxidant enzymes activities. Remarkably, Cuscuta australis (seed and stem) extract pretreatments in Groups 3, 4, 5, and 6, most especially, the stem extract pretreatment in Groups 5 and 6, improved better the hepatic histoarchitecture, the hepatocellular, and the oxidative stress injury markers in a dose-dependent manner. Conclusively, ethanol extractions of Cuscuta australis stem appear to protect the liver from acetaminophen intoxication better than the seed counterpart.

  7. Simulating the effect of emex australis densities and sowing dates on agronomic traits of wheat

    International Nuclear Information System (INIS)

    Abbas, R.N.; Tanveer, A.; Ali, A.; Zaheer, Z.A.

    2010-01-01

    Reduction in yield and quality of wheat is major problem caused by the delayed sowing and interference of weeds. The effects of sowing dates (Nov 8, Nov 16, and Nov 24) and Emex australis Steinh. density (0, 1, 2, 3, 4 plants per pot) on growth and yield of wheat were evaluated over two seasons (2005-06, 2006-07). The statistical analysis of data exhibited non-significant effect of weed density on number of days taken to initiate flowering by E. australis. Highest values for E. australis plant height, dry biomass, number of seed per plant and seed weight were recorded by sowing wheat on November 8, at E. australis density of one plant per pot in both years. Maximum number of spike bearing tillers per pot, plant height, number of grains per spike, 1000-grain weight and grain yield were observed in November 8, sowing with zero E. australis density and minimum values for these parameters were recorded in late sowing ( November 24) at maximum weed density of 4 plants per pot. Early sowing ( Nov. 8) and weed free pots increased wheat grain yield compared to later sowings (Nov. 16 and Nov. 24) and higher weed density. (author)

  8. Transcriptome Expression Profiling in Response to Drought Stress in Paulownia australis

    Directory of Open Access Journals (Sweden)

    Yanpeng Dong

    2014-03-01

    Full Text Available The response and adaptation to drought remains poorly understood for Paulownia australis. To investigate this issue, transcriptome profiling of four P. australis accessions (two diploid and the other two autotetraploid under water stress condition were studied using Illumina Genome Analyzer IIx analysis. The current study aimed to identify genes of P. australis metabolism pathways that might be involved in this plant’s response to water deficit. Potted seedlings were subjected to well-watered conditions and drought stress, respectively. More than 290 million raw transcript reads were assembled into 111,660 unigenes, with a mean length of 1013 bp. Clusters of orthologous groups, gene ontology and the Kyoto Encyclopedia of Genes and Genomes annotations analyses were performed on the unigenes. Many differentially expressed genes and several metabolic pathways were identified. Quantitative real-time polymerase chain reaction was used to verify the expression patterns of 14 genes. Our study identified altered gene expression in P. australis induced by drought stress and provided a comprehensive map of drought-responsive genes and pathways in this species. To our knowledge, this is the first publicly available global transcriptome study of P. australis. This study provides a valuable genetic resource for this species.

  9. Evaluation of methanolic extract of Phragmites karka on carbon tetrachloride-induced liver fibrosis in rat

    Directory of Open Access Journals (Sweden)

    Atta ur Rehman

    2017-07-01

    Full Text Available Phragmites karka has been reported for its anti-inflammatory and analgesic activities. Here, extracts of leaf and rhizome of the plant were individually investigated in CCl4-induced hepatofibrosis in male Wistar rats by administering CCl4 intraperitoneally biweekly for 6 weeks. Afterwards the animals were investigated for liver fibrosis at biochemical, molecular and histological levels, and it showed a profound increase (p<0.001 in elevation of serum levels of transaminases, γ-glutamyl transpeptidase, mRNA expression of α smooth muscle actin, collagen and transforming growth factor beta (TGFβ, and extracellular matrix deposition and perilobular necrosis. Both extracts markedly (p<0.001 decreased the elevated levels of these markers. Histopathological investigations also substantiated the above results by exhibiting a decreased in extracellular matrix deposition in post-treatment animals. In conclusion, both extracts had substantially modified the biochemical and molecular markers of liver fibrosis, in addition to histological improvement in architecture of liver.

  10. Effects of CO 2 concentration and moisture content of sugar-free media on the tissue-cultured plantlets in a large growth chamber

    Science.gov (United States)

    Qu, Y. H.; Lin, C.; Zhou, W.; Li, Y.; Chen, B.; Chen, G. Q.

    2009-01-01

    The dynamic fluctuations of CO 2 concentration in the tissue culture growth chamber after transplantation of petunia, chrysanthemum and tomato plantlets were recorded with a real-time control system to determine the critical CO 2 concentration levels of 35 μl l -1 at which CO 2 enrichment is needed. The experimental data showed that the tissue-cultured plantlets of petunia, chrysanthemum and tomato had the same CO 2 concentration dynamics. The results indicated that CO 2 enrichment was proper on the second day after transplantation. Petunia plantlets were used to conduct experiments under PPFD of 80 μmol m -2 s -1, and CO 2 concentrations of 350 ± 50 μl l -1, 650 ± 50 μl l -1 and 950 ± 50 μl l -1 as well as medium moisture contents of 60%, 70% and 80%, with the result that plantlets grew better under CO 2 concentration of 650 ± 50 μl l -1 than under the other two concentrations with all the different media water contents. Three media water contents under the same CO 2 concentration produced plantlets with the same quality. The impacts of CO 2 concentrations on plantlets are more important than those of the media water contents. Sugar-free tissue culture, as compared with the conventional culture, showed that CO 2 enrichment to 350 ± 50 μl l -1 can promote the growth of the cultured plantlets. Sugar-free tissue culture produced healthy plantlets with thick roots, almost equivalent to the common plantlets.

  11. Description of the immature stages of the planthopper Lacertinella australis (Hemiptera: Delphacidae)

    Science.gov (United States)

    Batiz, M. F. Rossi; Marino de Remes Lenicov, A. M.; Hagedorn, Henry

    2014-01-01

    Abstract The five immature stages of the planthopper Lacertinella australis (Remes Lenicov and Rossi Batiz) (Hemiptera: Delphacidae: Saccharosydnini) are described and illustrated. The main characters that allowed us to distinguish the various stages were body size, number of tarsomeres and metatibial spines, and number of teeth on the spur. New biological data based on laboratory rearing and field observations showed that L. australis can carry out its biological cycle successfully on the graminaceous pampas grass ( Cortaderia spp. Stapf (Poales: Poaceae)). In addition, the efficient rearing in captivity, the high survivorship registered, and overwintering only on this host plant suggests that L. australis is a potential biocontrol agent of this invasive graminaceous weed. This study provides information about the immature stages , including a key for their identification, based on laboratory reared specimens and field observations. Resumen Se describen e ilustran las cinco etapas inmaduras de la especie de Saccharisydnini Lacertinella australis (Remes Lenicov and Rossi Batiz) (Hemiptera: Delphacidae). Los principales caracteres que permitieron distinguir las diferentes etapas fueron: tamaño corporal, número de espinas en los tarsómeros y metatibia y número de dientes en el espolón tibial. Nuevos datos biológicos, basados en la cría de laboratorio y observaciones de campo, mostraron que L. australis puede realizar su ciclo biológico exitosamente en la graminácea cortadera ( Cortaderia spp. Stapf (Poales: Poaceae)). Además, la eficiente crianza en cautive-rio y la alta supervivencia registrada en esta planta hospedera, sugieren que L. australis podría ser usada como un potencial agente de control biológico de esta maleza invasora. Este estudio proporciona informa-ción sobre las etapas inmaduras, incluyendo una clave para su identificación, basada en individuos provenientes de la cría de laboratorio y de campo. PMID:25199992

  12. Exposure dose response relationships of the freshwater bivalve Hyridella australis to cadmium spiked sediments

    International Nuclear Information System (INIS)

    Marasinghe Wadige, Chamani P.M.; Maher, William A.; Taylor, Anne M.; Krikowa, Frank

    2014-01-01

    Highlights: • The exposure–dose–response approach was used to assess cadmium exposure and toxicity. • Accumulated cadmium in H. australis reflected the sediment cadmium exposure. • Spill over of cadmium into the biologically active pool was observed. • Increased cadmium resulted in measurable biological effects. • H. australis has the potential to be a cadmium biomonitor in freshwater environments. - Abstract: To understand how benthic biota may respond to the additive or antagonistic effects of metal mixtures in the environment it is first necessary to examine their responses to the individual metals. In this context, laboratory controlled single metal-spiked sediment toxicity tests are useful to assess this. The exposure–dose–response relationships of Hyridella australis to cadmium-spiked sediments were, therefore, investigated in laboratory microcosms. H. australis was exposed to individual cadmium spiked sediments (<0.05 (control), 4 ± 0.3 (low) and 15 ± 1 (high) μg/g dry mass) for 28 days. Dose was measured as cadmium accumulation in whole soft body and individual tissues at weekly intervals over the exposure period. Dose was further examined as sub-cellular localisation of cadmium in hepatopancreas tissues. The biological responses in terms of enzymatic and cellular biomarkers were measured in hepatopancreas tissues at day 28. H. australis accumulated cadmium from spiked sediments with an 8-fold (low exposure organisms) and 16-fold (high exposure organisms) increase at day 28 compared to control organisms. The accumulated tissue cadmium concentrations reflected the sediment cadmium exposure at day 28. Cadmium accumulation in high exposure organisms was inversely related to the tissue calcium concentrations. Gills of H. australis showed significantly higher cadmium accumulation than the other tissues. Accumulated cadmium in biologically active and biologically detoxified metal pools was not significantly different in cadmium exposed

  13. A Proteome Translocation Response to Complex Desert Stress Environments in Perennial Phragmites Sympatric Ecotypes with Contrasting Water Availability.

    Science.gov (United States)

    Li, Li; Chen, Xiaodan; Shi, Lu; Wang, Chuanjing; Fu, Bing; Qiu, Tianhang; Cui, Suxia

    2017-01-01

    After a long-term adaptation to desert environment, the perennial aquatic plant Phragmites communis has evolved a desert-dune ecotype. The desert-dune ecotype (DR) of Phragmites communis showed significant differences in water activity and protein distribution compared to its sympatric swamp ecotype (SR). Many proteins that were located in the soluble fraction of SR translocated to the insoluble fraction of DR, suggesting that membrane-associated proteins were greatly reinforced in DR. The unknown phenomenon in plant stress physiology was defined as a proteome translocation response. Quantitative 2D-DIGE technology highlighted these 'bound' proteins in DR. Fifty-eight kinds of proteins were identified as candidates of the translocated proteome in Phragmites . The majority were chloroplast proteins. Unexpectedly, Rubisco was the most abundant protein sequestered by DR. Rubisco activase, various chaperons and 2-cysteine peroxiredoxin were major components in the translocation response. Conformational change was assumed to be the main reason for the Rubisco translocation due to no primary sequence difference between DR and SR. The addition of reductant in extraction process partially reversed the translocation response, implying that intracellular redox status plays a role in the translocation response of the proteome. The finding emphasizes the realistic significance of the membrane-association of biomolecule for plant long-term adaptation to complex stress conditions.

  14. Micropropagation of an Exotic Ornamental Plant, Calathea crotalifera, for Production of High Quality Plantlets

    Directory of Open Access Journals (Sweden)

    Shahril Efzueni Rozali

    2014-01-01

    Full Text Available A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induction rate of shoots with lowest contamination percentage when treated with combination of 30% (v/v NaOCl, 70% (v/v ethanol, and 0.3% (w/v HgCl2. In the present study, the highest number of multiple shoots was obtained in MS basal medium supplemented with 3.5 mg/L 6-Benzylaminopurine (BAP, 1.0 mg/L 1-Naphthaleneacetic acid (NAA, 3% sucrose, and 6 g/L plant agar for both varieties and was used as multiplication medium. Microshoots were highly induced when the young shoot bud explants were incised longitudinally prior subculture. Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L 1-Naphthaleneacetic acid (NAA compared to indolebutyric acid (IBA. The complete regenerated plantlets were successfully acclimatized in the soilless medium under greenhouse condition. This is the first report of rapid mass propagation for C. crotalifera.

  15. Micropropagation of an exotic ornamental plant, Calathea crotalifera, for production of high quality plantlets.

    Science.gov (United States)

    Rozali, Shahril Efzueni; Rashid, Kamaludin A; Taha, Rosna Mat

    2014-01-01

    A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induction rate of shoots with lowest contamination percentage when treated with combination of 30% (v/v) NaOCl, 70% (v/v) ethanol, and 0.3% (w/v) HgCl2. In the present study, the highest number of multiple shoots was obtained in MS basal medium supplemented with 3.5 mg/L 6-Benzylaminopurine (BAP), 1.0 mg/L 1-Naphthaleneacetic acid (NAA), 3% sucrose, and 6 g/L plant agar for both varieties and was used as multiplication medium. Microshoots were highly induced when the young shoot bud explants were incised longitudinally prior subculture. Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L 1-Naphthaleneacetic acid (NAA) compared to indolebutyric acid (IBA). The complete regenerated plantlets were successfully acclimatized in the soilless medium under greenhouse condition. This is the first report of rapid mass propagation for C. crotalifera.

  16. Micropropagation of herbal plants for mass production of in vitro plantlets

    Energy Technology Data Exchange (ETDEWEB)

    Hussein, Sobri; Ibrahim, Rusli [Malaysian Institute for Nuclear Technology Research MINT, Bangi (Malaysia). Agrotechnology and Biosciences Div.

    2006-09-15

    Malaysia herbal industry is considered to be one of the most dynamic enterprises with annual growth estimated at 20 % a year. The total import value of the medicinal and aromatic plants increased from RM 141 million in 1986 to RM 431 million in 1996. Species that have been identified in terms of current priority are Eurycoma longifolia (Tongkat Ali), Labisia pumila (Kacip Fatimah), Andrographis paniculata (Hempedu Bumi), Morinda citrifolia (Mengkudu), Centella asiatica (Pegaga), Orthosiphon aristatus (Misai Kucing) and Gynura procumbens (Sambung Nyawa). Herbal and medicinal plants have a larger pool of genetic resources for the production of compounds valuable to the industry and human well being such as in pharmaceuticals, nutraceuticals and health food. The use of tissue culture technology has long been considered as an attractive solution to the problem of limited supply of raw materials for the industries. Kacip Fatimah (Labisia pumila) has been well known to increase female hormone, overcome tiredness and regain overall health. Misai Kucing (Orthosiphon aristatus) has proven to be active against kidney stones, high blood pressure, diabetes and gout and Sambung Nyawa (Gynura procumbens) is active against hypertension, diabetes and cancer. Tissue culture protocols had been optimized for the mass production of in vitro plantlets of these three selected herbal plants. (Author)

  17. Interaction of Ulocladium atrum, a Potential Biological Control Agent, with Botrytis cinerea and Grapevine Plantlets

    Directory of Open Access Journals (Sweden)

    Sébastien Ronseaux

    2013-09-01

    Full Text Available The effectiveness of biological control agent, Ulocladium atrum (isolates U13 and U16 in protecting Vitis vinifera L. cv. Chardonnay against gray mold disease caused by Botrytis cinerea, and simulation of the foliar defense responses was investigated. A degraded mycelium structure during cultural assay on potato dextrose agar revealed that U. atrum isolates U13 and U16 were both antagonistic to B. cinerea, mainly when isolates were inoculated two days before Botrytis. Under in vitro conditions, foliar application of U. atrum protected grapevine leaves against gray mold disease. An increase in chitinase activity was induced by the presence of U. atrum isolates indicating that the biological control agents triggered plant defense mechanisms. Moreover, U13 has the potential to colonize the grapevine plantlets and to improve their growth. The ability of U. atrum isolates to exhibit an antagonistic effect against B. cinerea in addition to their aptitude to induce plant resistance and to promote grapevine growth may explain a part of their biological activity. Hence, this study suggests that U. atrum provides a suitable biocontrol agent against gray mold in grapevines.

  18. Micropropagation of herbal plants for mass production of in vitro plantlets

    International Nuclear Information System (INIS)

    Sobri Hussein; Rusli Ibrahim

    2006-01-01

    Malaysia herbal industry is considered to be one of the most dynamic enterprises with annual growth estimated at 20 % a year. The total import value of the medicinal and aromatic plants increased from RM 141 million in 1986 to RM 431 million in 1996. Species that have been identified in terms of current priority are Eurycoma longifolia (Tongkat Ali), Labisia pumila (Kacip Fatimah), Andrographis paniculata (Hempedu Bumi), Morinda citrifolia (Mengkudu), Centella asiatica (Pegaga), Orthosiphon aristatus (Misai Kucing) and Gynura procumbens (Sambung Nyawa). Herbal and medicinal plants have a larger pool of genetic resources for the production of compounds valuable to the industry and human well being such as in pharmaceuticals, nutraceuticals and health food. The use of tissue culture technology has long been considered as an attractive solution to the problem of limited supply of raw materials for the industries. Kacip Fatimah (Labisia pumila) has been well known to increase female hormone, overcome tiredness and regain overall health. Misai Kucing (Orthosiphon aristatus) has proven to be active against kidney stones, high blood pressure, diabetes and gout and Sambung Nyawa (Gynura procumbens) is active against hypertension, diabetes and cancer. Tissue culture protocols had been optimized for the mass production of in vitro plantlets of these three selected herbal plants. (Author)

  19. Growing Tomato Plantlets on Various Mixtures of Peat and Sand or Peat and Perlite. Note 1

    Directory of Open Access Journals (Sweden)

    Antonia Patruno

    Full Text Available Given the considerable interest in use of substrates derived from various mixtures in the nursery sector and in light of the enormous variety of possibilities offered by this technique, in contrast with the still small number of researches dedicated to this theme, this study was set out to examine in-depth the growing of tomato plantlets on peat-based substrates. Two series of peat mixtures were produced, one with sand and the other with perlite, with a volume ratio of the other two components with respect to the peat of 1:0, 2.5:1, 1:1 and 1:2.5. Tomato seedlings were cultivated for 30 or 25 days in small perforated pots containing these mixtures. The irrigation was calculated by weighing each pot daily, measuring the water lost by evaporation-transpiration, then just past an established lower threshold value bringing the water back up to a defined upper threshold. Two water regimes were compared in the sand series and three in the perlite series.

  20. Discovery of a new subclass of alpha-conotoxins in the venom of Conus australis

    Digital Repository Service at National Institute of Oceanography (India)

    Lebbe, E; Peigneur, S.; Maiti, M.; Mille, B.G.; Prabhadevi; Ravichandran, S.; Lescrinier, E; Waelkens, E; DeSouza, L.; Herdewijn, P.; Tytgat, J.

    -conotoxin family. We purified the venom of a yet unexplored cone snail species, i.e. Conus australis, and we isolated a 16-amino acid peptide named alpha-conotoxin AusIA. The peptide has the typical alpha-conotoxin CC-Xm-C-Xn-C framework...

  1. Primary structure of the oligosaccharide moiety of hemocyanin from the scorpion Androctonus australis

    NARCIS (Netherlands)

    Vliegenthart, J.F.G.; Debeire, P.; Montreuil, J.; Goyffon, M.; Kuik, J.A. van; Halbeek, H. van

    1986-01-01

    Hemocyanin, the copper-containing glycoprotein that serves as an oxygen carrier in the hemolymph of some arthropods and molluscs, was obtained from the blood of the scorpion Androctonus australis. Sugar analysis of the glycoprotein revealed that its carbohydrate moiety is of the N-glycosylic type.

  2. PEMANFAATAN GULMA AIR PERUPUK (Phragmites karka Trin SEBAGAI TUMBUHAN PEREDUKSI LIMBAH CAIR INDUSTRI KARET

    Directory of Open Access Journals (Sweden)

    Deddy Dharmaji

    2016-09-01

    Full Text Available This research was aimed analyze the ability of perupuk (Phragmites karka Trin in reducing of rubber industrial liquid waste polluters on the scale of the laboratory. The method used was the method of survey. The data laboratory test were tabulated and analyzed descriptively and the level of efficiency was calculated. Referring to South Kalimantan Governor Regulation Number 36/2008, the results showed that, TSS parameters started to be effectively reduced on day 10 with close to 84,33 mg/l (32.53%, BOD5 started to be effectively reduced on day 20 with close to 24.00 mg/l (99,29%, and COD started to be effectively reduced on day 20 with close to 44,65 mg/l (98,90%. Referring to the decision of the Minister of Environment No. KEP-78/MENLH/10/1995, the parameters of the Sulfide started to be effectively reduced on day 10 with close to 0.001 mg/l (93,75% and Manganese start to be effectively reduced on day 20 with close to 0,70 mg/l (-134,00 %. Ph levels and temperature still normal condition.  Time retention was best accomplished on day 30 in reducing liquid waste rubber industry.

  3. Selection of suitable propagation method for consistent plantlets production in Stevia rebaudiana (Bertoni)

    Science.gov (United States)

    Khalil, Shahid Akbar; Zamir, Roshan; Ahmad, Nisar

    2014-01-01

    Stevia rebaudiana (Bert.) is an emerging sugar alternative and anti-diabetic plant in Pakistan. That is why people did not know the exact time of propagation. The main objective of the present study was to establish feasible propagation methods for healthy biomass production. In the present study, seed germination, stem cuttings and micropropagation were investigated for higher productivity. Fresh seeds showed better germination (25.51–40%) but lost viability after a few days of storage. In order to improve the germination percentage, seeds were irradiated with 2.5, 5.0, 7.5 and 10 Gy gamma doses. But gamma irradiation did not show any significant change in seed germination. A great variation in survival of stem cutting was observed in each month of 2012. October and November were found the most suitable months for stem cutting survival (60%). In order to enhance survival, stem cuttings were also dipped in different plant growth regulators (PGRs) solution. Only indole butyric acid (IBA; 1000 ppm) treated cutting showed a higher survival (33%) than control (11.1%). Furthermore, simple and feasible indirect regeneration system was established from leaf explants. Best callus induction (84.6%) was observed on MS-medium augmented with 6-benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D; 2.0 mg l−1). For the first time, we obtained the highest number of shoots (106) on a medium containing BA (1.5 mg l−1) and gibberellic acid (GA3; 0.5 mg l−1). Plantlets were successfully acclimatized in plastic pots. The current results preferred micropropagation (85%) over seed germination (25.51–40%) and stem cutting (60%). PMID:25473365

  4. Direct plantlet inoculation with soil or insect-associated fungi may control cabbage root fly maggots.

    Science.gov (United States)

    Razinger, Jaka; Lutz, Matthias; Schroers, Hans-Josef; Palmisano, Marilena; Wohler, Christian; Urek, Gregor; Grunder, Jürg

    2014-07-01

    A potential Delia radicum biological control strategy involving cauliflower plantlet inoculation with various fungi was investigated in a series of laboratory and glasshouse experiments. In addition to entomopathogenic fungi, fungi with a high rhizosphere competence and fungi with the ability to survive as saprotrophs in soil were tested. The following fungal species were evaluated in the experiments: Trichoderma atroviride, T. koningiopsis, T. gamsii, Beauveria bassiana, Metharhizium anisopliae, M. brunneum and Clonostachys solani. A commercial carbosulfan-based insecticide was used as a positive control. Additionally, two commercial products, one based on B. bassiana (Naturalis) and one on Bacillus thuringiensis (Delfin) were used as reference biocontrol agents. The aims were (i) to assess the pathogenicity of the selected fungal isolates to Delia radicum, (ii) to evaluate the fungal isolates' rhizosphere competence, with the emphasis on the persistence of the original inoculum on the growing roots, (iii) to assess possible endophytic plant tissue colonization, and (iv) to evaluate potential plant growth stimulating effects of the added inoculi. Significant pathogenicity of tested fungi against Delia radicum was confirmed in in vitro and glasshouse experiments. All tested fungi persisted on cauliflower rhizoplane. More importantly, the added fungi were found on thoroughly washed roots outside the original point of inoculation. This provided us with evidence that our tested fungi could be transferred via or grow with the elongating roots. In addition to colonizing the rhizoplane, some fungi were found inside the plant root or stem tissue, thus exhibiting endophytic characteristics. The importance of fungal ecology as a criterion in appropriate biological control agent selection is discussed. Copyright © 2014 Elsevier Inc. All rights reserved.

  5. Selection of suitable propagation method for consistent plantlets production in Stevia rebaudiana (Bertoni).

    Science.gov (United States)

    Khalil, Shahid Akbar; Zamir, Roshan; Ahmad, Nisar

    2014-12-01

    Stevia rebaudiana (Bert.) is an emerging sugar alternative and anti-diabetic plant in Pakistan. That is why people did not know the exact time of propagation. The main objective of the present study was to establish feasible propagation methods for healthy biomass production. In the present study, seed germination, stem cuttings and micropropagation were investigated for higher productivity. Fresh seeds showed better germination (25.51-40%) but lost viability after a few days of storage. In order to improve the germination percentage, seeds were irradiated with 2.5, 5.0, 7.5 and 10 Gy gamma doses. But gamma irradiation did not show any significant change in seed germination. A great variation in survival of stem cutting was observed in each month of 2012. October and November were found the most suitable months for stem cutting survival (60%). In order to enhance survival, stem cuttings were also dipped in different plant growth regulators (PGRs) solution. Only indole butyric acid (IBA; 1000 ppm) treated cutting showed a higher survival (33%) than control (11.1%). Furthermore, simple and feasible indirect regeneration system was established from leaf explants. Best callus induction (84.6%) was observed on MS-medium augmented with 6-benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D; 2.0 mg l(-1)). For the first time, we obtained the highest number of shoots (106) on a medium containing BA (1.5 mg l(-1)) and gibberellic acid (GA3; 0.5 mg l(-1)). Plantlets were successfully acclimatized in plastic pots. The current results preferred micropropagation (85%) over seed germination (25.51-40%) and stem cutting (60%).

  6. BAROMETRIC PRESSURE and Other Data from AURORA AUSTRALIS and Other Platforms from 19910106 to 19920306 (NODC Accession 9500152)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Conductivity, Temperature and Depth (CTD) and other data were collected from Ship AURORA AUSTRALIS. The data was collected over a period spanning from January 6,...

  7. Pembentukan mother plant Bacopa australis secara In-vitro dan aklimatisasi dalam aquascape air tawar

    Directory of Open Access Journals (Sweden)

    Media Fitri Isma Nugraha

    2017-12-01

    Full Text Available Tanaman air adalah bagian penting dari ekosistem air tawar. Salah satu spesies yang terkenal adalah Bacopa australis. Hobiis aquascape saat ini memiliki ketertarikan tinggi terhadap tanaman air dengan kualitas yang bagus dari setiap spesiesnya. Metode perbanyakan tanaman air tanpa tanah, lahan pertanian dan air perlu dilakukan untuk memenuhi keinginan tersebut. Tujuan dari penelitian ini adalah untuk mendapatkan formula media kultur jaringan dan zat pengatur tumbuh yang tepat untuk multiplikasi dalam perakitan mother plant (tanaman induk Bacopa australis, serta mendapatkan media terbaik untuk aklimatisasi. Media yang digunakan adalah media Murashige dan Skoog (MS A padat dengan perbedaan konsentrasi zat pengatur tumbuh. Perlakuan uji dalam kombinasi zat pengatur tumbuh (a 0,50 mg/L BAP + 0,50 mg/L kinetin; (b 0,50 mg/L BAP; dan (c 0,50 mg/L 2,4-D. Aklimatisasi tanaman induk dilakukan pada berbagai media antara lain 1 pasir silika + pupuk aqua soil amazonia, 2. pasir malang + pupuk aqua soil amazonia, 3 pasir silika + pupuk cair; 4 pasir malang + pupuk. Hasil yang diperoleh, yaitu formula media kultur terbaik untuk multiplikasi tunas tanaman B. australis secara in-vitro adalah media MS (A yang diperkaya dengan 0,5 mg/L BAP + 0,5 mg/L kinetin, sedangkan aklimatisasi terbaik pada media pasir malang + pupuk aqua soil amazonia. Water plant is an important part of freshwater ecosystems. One of the famous species is Bacopa australis. Today, many aquascape hobbyists have a high interest in aquatic plant species that have good aesthetic appearances. To answer this challenge, a new method in-vitro propagation of aquatic plants, planted without soil, agricultural land and water was conducted. The aim of this research was to find the best growth regulator hormon formula and aclimatisation medium, in creating the mother plant Bacopa australis. The medium used was MS (Murashige and Skoog, 1974 with different growth regulator hormon, i.e: (a 0.50 mg L-1 BAP

  8. Different exogenous sugars affect the hormone signal pathway and sugar metabolism in "Red Globe" (Vitis vinifera L.) plantlets grown in vitro as shown by transcriptomic analysis.

    Science.gov (United States)

    Mao, Juan; Li, Wenfang; Mi, Baoqin; Dawuda, Mohammed Mujitaba; Calderón-Urrea, Alejandro; Ma, Zonghuan; Zhang, Yongmei; Chen, Baihong

    2017-09-01

    Exogenously applied 2% fructose is the most appropriate carbon source that enhances photosynthesis and growth of grape plantlets compared with the same concentrations of sucrose and glucose. The role of the sugars was regulated by the expression of key candidate genes related to hormones, key metabolic enzymes, and sugar metabolism of grape plantlets ( Vitis vinifera L.) grown in vitro. The addition of sugars including sucrose, glucose, and fructose is known to be very helpful for the development of grape (V. vinifera L.) plantlets in vitro. However, the mechanisms by which these sugars regulate plant development and sugar metabolism are poorly understood. In grape plantlets, sugar metabolism and hormone synthesis undergo special regulation. In the present study, transcriptomic analyses were performed on grape (V. vinifera L., cv. Red Globe) plantlets in an in vitro system, in which the plantlets were grown in 2% each of sucrose (S20), glucose (G20), and fructose (F20). The sugar metabolism and hormone synthesis of the plantlets were analyzed. In addition, 95.72-97.29% high-quality 125 bp reads were further analyzed out of which 52.65-60.80% were mapped to exonic regions, 13.13-28.38% to intronic regions, and 11.59-28.99% to intergenic regions. The F20, G20, and S20 displayed elevated sucrose synthase (SS) activities; relative chlorophyll contents; Rubisco activity; and IAA and zeatin (ZT) contents. We found F20 improved the growth and development of the plantlets better than G20 and S20. Sugar metabolism was a complex process, which depended on the balanced expression of key potential candidate genes related to hormones (TCP15, LOG3, IPT3, ETR1, HK2, HK3, CKX7, SPY, GH3s, MYBH, AGB1, MKK2, PP2C, PYL, ABF, SnRK, etc.), key metabolic enzymes (SUS, SPS, A/V-INV, and G6PDH), and sugar metabolism (BETAFRUCT4 and AMY). Moreover, sugar and starch metabolism controls the generation of plant hormone transduction pathway signaling molecules. Our dataset advances our

  9. Competitive-IgY- Enzyme Linked Immuno Sorbent Assay (CIgY-ELISA to detect the cytokinins in Gerbera jamesonii plantlets

    Directory of Open Access Journals (Sweden)

    Cleiton Mateus Sousa

    2011-08-01

    Full Text Available A competitive hyper-immune yolk Immunoglobulin Y - Enzyme Linked Immune Sorbent Assay (CIgY-ELISA, was developed as an alternative method to detect zeatin and 2ip in plantlets of gerbera. The endogenous level of hormones in the plantlets in vitro of gerbera with one or six weeks after replication was determined with competitive IgY-ELISA set to detect between 1 and 100 pmoles of plant hormone for each 1.0 g tissue. The plantlets of six weeks presented sprouts and root, while the plantlets of one week presented only sprouts. The CIgY-ELISA was set with high independent variables values of sensitivity/specificity of 96/89 % for zeatin and 94/78 % for 2ip, with high values of reproducibility (up to 90 % for both the cytokinins. Zeatin content varied from 2.2 to 2.8 pmoles.g-1 and from 2.7 to 3.3 pmoles.g-1 on the plantlet with one and six weeks, respectively. The 2ip content did not vary and was detected near the detection limit in all the assays. It was concluded that the observed capabilities of CIgY-ELISA were putative and the competitive assay was a highly robust and stable method, which could be used for the studies on plant physiology for endogenous cytokinins.

  10. Reversion of hyperhydricity in pink (Dianthus chinensis L.) plantlets by AgNO3 and its associated mechanism during in vitro culture.

    Science.gov (United States)

    Gao, Hongyang; Xia, Xiuying; An, Lijia; Xin, Xin; Liang, Yuan

    2017-01-01

    Hyperhydricity occurs frequently in plant tissue culture and can severely affect commercial micropropagation and genetic improvement of the cultured plantlets. Hyperhydric shoots are charaterzized by high water content, but how this occurs is still a subject of investigation. Silver ion (Ag + ) can reduce the extent of hyperhydricity in plants, but its effect on the reversion of hyperhydric plantlets and the underlying mechanism of reversion has not been clarified. In this study, about 67% of the hyperhydric Dianthus chinensis L. plantlets were found to revert to normal condition when the plantlets were cultured in medium supplemented with 29.4μmolL -1 AgNO 3 . Water content and hydrogen peroxide (H 2 O 2 ) content in the guard cells of these plantlets were reduced, while stomatal aperture and water loss rate were increased. AgNO 3 also reduced the content of endogenous ethylene and expression of ethylene synthesis and ethylene signal transduction-associated genes. Reduced accumulation of ethylene consequently led to an increase in stomatal aperture mediated by decreased H 2 O 2 content in the guard cells. These results adequately verified the role of AgNO 3 in the reversion of hyperhydricity in D. chinensis L. and also provided clues for exploring the cause of excessive water accumulation in hyperhydric plants. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  11. Relevance of Allergenic Sensitization to Cynodon dactylon and Phragmites communis: Cross-reactivity With Pooideae Grasses.

    Science.gov (United States)

    López-Matas, M A; Moya, R; Cardona, V; Valero, A; Gaig, P; Malet, A; Viñas, M; García-Moral, A; Labrador, M; Alcoceba, E; Ibero, M; Carnés, J

    The homologous group of sweet grasses belongs to the Pooideae subfamily, but grass pollen species from other subfamilies can also cause allergy, such as Cynodon dactylon (Chloridoideae) and Phragmites communis (Arundinoideae). C dactylon and P communis have not been included in the sweet grasses homologous group because of their low cross-reactivity with other grasses. The aims of this study were to investigate the profile of sensitization to C dactylon and P communis in patients sensitized to grasses and to analyze cross-reactivity between these 2 species and temperate grasses. Patients were skin prick tested with a grass mixture (GM). Specific IgE to GM, C dactylon, P communis, Cyn d 1, and Phl p 1 was measured by ImmunoCAP. A pool of sera was used for the immunoblot assays. Cross-reactivity was studied by ELISA and immunoblot inhibition. Thirty patients had sIgE to GM. Twenty-four (80%) had positive results for C dactylon, 27 (90%) for P communis, 22 (73.3%) for nCyn d 1, and 92.9% for rPhl p 1. Bands were detected in the 3 extracts by immunoblot. Inhibition of GM was not observed with C dactylon or P communis by immunoblot or ELISA inhibition. When C dactylon or P communis were used in the solid phase, GM produced almost complete inhibition. Eighty percent of patients sensitized to grasses were also sensitized to C dactylon and 90% were sensitized to P communis. Sensitization to these species seems to be induced by allergens different to those in sweet grasses.

  12. Induction of embryogenic callus and plantlet regeneration from young leaves of high yielding mature oil palm

    Directory of Open Access Journals (Sweden)

    Yeedum, I.

    2004-09-01

    Full Text Available Callus induction and plantlet regeneration from young leaves of high-yielding mature oil palm were carried out using 10-year and 20-year-old trees from Thepa Research Station, Faculty of Natural Resources,Prince of Songkla University, Hat Yai, and Trang Agricultural College, respectively. Culture media used in this experiment were Murashige and Skoog (1962 and Oil Palm supplemented with various concentrations of α-naphthaleneacetic acid (NAA or 2,4- dichlorophenoxy acetic acid (2,4-D or dicamba (Di and antioxidants.Young leaves from 6th to 11st frond were excised, sterilized, cut into 5x5 mm pieces and cultured in the dark at 26±4ºC or 28±0.5ºC for 3 months. The results revealed that MS medium with 200 mg/l ascorbic acid (As and 1 mg/l Di (MS-AsDi gave the highest callus induction percentage (7.93 after culture for 3 months at 28±0.5ºC. Leaf segments from 6th - 8th frond yielded callus forming percentage at 10% (averaged from 1, 2.5 and 5 mg/l Di containing MS medium. Ascorbic acid as an antioxidant at concentration of 200 mg/l supplemented in MS medium in the presence of 2.5 mg/l Di produced the highest callus induction percentage (11.2 and number of nodules (7.06. A high percentage of embryogenic callus formation (66.67 was obtained when the calli were transferred to the same medium component supplemented with 0.5 mg/l Di and 1,000 mg/l casein hydrolysate (CH (MS-AsDiCH. Haustorial-staged embryos were observed to be isolated as an individual embryo and germinated on MS medium without plant growth regulator (MS-free. Development of root could be classified into two distinct types, fibrous and tap root.

  13. Chonopeltis australis (Crustacea) male reproductive system morphology; sperm transfer and review of reproduction in Branchiura.

    Science.gov (United States)

    Neethling, Lourelle Alicia Martins; Avenant-Oldewage, Annemariè

    2015-02-01

    The morphology of the male reproductive system as well as sperm transfer in Branchiura has been described for Dolops ranarum and Argulus japonicus. In this study, the reproductive system and accessory structures are described for male Chonopeltis australis using histology, light microscopy, and scanning electron microscopy. For the first time, we describe sperm transfer by means of a spermatophore in this genus. The internal and external morphology and mechanism of sperm transfer is compared with other Branchiura, where it has been described. The morphology of the reproductive system of C. australis is similar to that of D. ranarum while the accessory structures and the spermatophore produced are similar to that of A. japonicus. A revision of the definition of Branchiura with respect to reproduction is provided. © 2014 Wiley Periodicals, Inc.

  14. Understanding Super-Earths with MINERVA-Australis at USQ's Mount Kent Observatory

    Science.gov (United States)

    Wittenmyer, Robert; Horner, Jonathan; Kane, Stephen; Plavchan, Peter; Ciardi, David; Eastman, Jason; Johnson, John Asher; Wright, Jason; McCrady, Nate; MINERVA Collaboration

    2018-01-01

    Super Earths, planets between 5-10 Earth masses, are the most common types of planets known, yet are completely absent from our Solar system. As a result, their detailed properties, compositions, and formation mechanisms are poorly understood. NASA's Transiting Exoplanet Survey Satellite (TESS) will identify hundreds of Super-Earths orbiting bright stars, for the first time allowing in-depth characterisation of these planets. At the University of Southern Queensland, we are host to the MINERVA-Australis project, dedicated wholly to the follow-up characterisation and mass measurement of TESS planets. We give an update on the status of MINERVA-Australis and our expected performance. We also present results from the fully operational Northern MINERVA array, with the primary mission of discovering rocky planets orbiting 80 nearby bright stars.

  15. [Effects of Cuscuta australis parasitism on the growth, reproduction and defense of Solidago canadensis].

    Science.gov (United States)

    Yang, Bei-fen; Du, Le-shan; Li, Jun-min

    2015-11-01

    In order to find out how parasitic Cuscuta australis influences the growth and reproduction of Solidago canadensis, the effects of the parasitism of C. australis on the morphological, growth and reproductive traits of S. canadensis were examined and the relationships between the biomass and the contents of the secondary metabolites were analyzed. The results showed that the parasitism significantly reduced the plant height, basal diameter, root length, root diameter, root biomass, stem biomass, leaf biomass, total biomass, number of inflorescences branches, axis length of inflorescence, and number of inflorescence. In particular, plant height, number of inflorescence and the stem biomass of parasitized S. canadensis were only 1/2, 1/5 and 1/8 of non-parasitized plants, respectively. There was no significant difference of plant height, root length, stem biomass and total biomass between plants parasitized with high and low intensities. But the basal diameter, root volume, leaf biomass, root biomass, the number of inflorescences branches, axis length of inflorescence and number of inflorescence of S. canadensis parasitized with high intensity were significantly lower than those of plants parasitized with low intensity. The parasitism of C. australis significantly increased the tannins content in the root and the flavonoids content in the stem of S. canadensis. The biomass of S. canadensis was significantly negatively correlated with the tannin content in the root and the flavonoids content in the stem. These results indicated that the parasitism of C. australis could inhibit the growth of S. canadensis by changing the resources allocation patterns as well as reducing the resources obtained by S. canadensis.

  16. A sulphated flavone glycoside from Livistona australis and its antioxidant and cytotoxic activity.

    Science.gov (United States)

    Kassem, Mona E S; Shoela, Soha; Marzouk, Mona M; Sleem, Amany A

    2012-01-01

    A new flavone glycoside tricin 7-O-β-glucopyranoside-2″-sulphate sodium salt along with 14 known flavonoid compounds were isolated and identified from the aqueous methanol extract of Livistona australis leaves. Their structures were established on the basis of extensive NMR (¹H, ¹³C, HSQC and H-H COSY) and ESIMS data. Antioxidant and cytotoxicity properties of the methanol extract of the leaves as well as the new compound were investigated.

  17. Short-term Responses of Posidonia australis to Changes in Light Quality

    Directory of Open Access Journals (Sweden)

    Simone Strydom

    2018-01-01

    Full Text Available Seagrass meadows are highly productive ecosystems that provide ecosystem services to the coastal zone but are declining globally, particularly due to anthropogenic activities that reduce the quantity of light reaching seagrasses, such as dredging, river discharge and eutrophication. Light quality (the spectral composition of the light is also altered by these anthropogenic stressors as the differential attenuation of wavelengths of light is caused by materials within the water column. This study addressed the effect of altered light quality on different life-history stages of the seagrass Posidonia australis, a persistent, habitat-forming species in Australia. Aquarium-based experiments were conducted to determine how adult shoots and seedlings respond to blue (peak λ = 451 nm; green (peak λ = 522 nm; yellow (peak λ = 596 nm and red (peak λ = 673 nm wavelengths with a control of full-spectrum light (λ = 400 – 700 nm, at 200 μmol photons m-2 s-1. Posidonia australis adults did not respond to changes in light quality relative to full-spectrum light, demonstrating a capacity to obtain enough photons from a range of wavelengths across the visible spectrum to maintain short-term growth at high irradiances. Posidonia australis seedlings (<4 months old grown in blue light showed a significant increase in xanthophyll concentrations when compared to plants grown in full-spectrum, demonstrating a pigment acclimation response to blue light. These results differed significantly from negative responses to changes in light quality recently described for Halophila ovalis, a colonizing seagrass species. Persistent seagrasses such as P. australis, appear to be better at tolerating short-term changes in light quality compared to colonizing species when sufficient PPFD is present.

  18. Non-linear growth in tree ferns, Dicksonia antarctica and Cyathea australis

    OpenAIRE

    Blair, David P.; Blanchard, Wade; Banks, Sam C.; Lindenmayer, David B.

    2017-01-01

    Tree ferns are an important structural component of forests in many countries. However, because their regeneration is often unrelated to major disturbances, their age is often difficult to determine. In addition, rates of growth may not be uniform, which further complicates attempts to determine their age. In this study, we measured 5 years of growth of Cyathea australis and Dicksonia antarctica after a large wildfire in 2009 in south-eastern Australia. We found growth rates of these two spec...

  19. Anatomy of nasal complex in the southern right whale, Eubalaena australis (Cetacea, Mysticeti).

    Science.gov (United States)

    Buono, Mónica R; Fernández, Marta S; Fordyce, R Ewan; Reidenberg, Joy S

    2015-01-01

    The nasal region of the skull has undergone dramatic changes during the course of cetacean evolution. In particular, mysticetes (baleen whales) conserve the nasal mammalian pattern associated with the secondary function of olfaction, and lack the sound-producing specializations present in odontocetes (toothed whales, dolphins and porpoises). To improve our understanding of the morphology of the nasal region of mysticetes, we investigate the nasal anatomy, osteology and myology of the southern right whale, Eubalaena australis, and make comparisons with other mysticetes. In E. australis external deflection surfaces around the blowholes appear to divert water off the head, and differ in appearance from those observed in balaenopterids, eschrichtiids and cetotherids. In E. australis the blowholes are placed above hypertrophied nasal soft tissues formed by fat and nasal muscles, a pattern also observed in balaenopterids (rorqual mysticetes) and a cetotherid (pygmy right whale, Caperea marginata). Blowhole movements are due to the action of five nasofacial muscles: dilator naris superficialis, dilator naris profundus, depressor alae nasi, constrictor naris, and retractor alae nasi. The dilator naris profundus found in E. australis has not been previously reported in balaenopterids. The other nasofacial muscles have a similar arrangement in balaenopterids, with minor differences. A novel structure, not reported previously in any mysticete, is the presence of a vascular tissue (rete mirabile) covering the lower nasal passage. This vascular tissue could play a role in warming inspired air, or may engorge to accommodate loss of respiratory space volume due to gas compression from increased pressure during diving. © 2014 Anatomical Society.

  20. DNA hybridization evidence for the Australasian affinity of the American marsupial Dromiciops australis.

    OpenAIRE

    Kirsch, J A; Dickerman, A W; Reig, O A; Springer, M S

    1991-01-01

    DNA hybridization was used to compare representatives of the major groups of marsupials and a eutherian outgroup. Because of the large genetic distances separating marsupial families, trees were calculated from normalized percentages of hybridization; thermal-melting statistics, however, gave identical topologies for the well-supported clades. The most notable results were the association of the only extant microbiotheriid, Dromiciops australis, an American marsupial, with the Australasian Di...

  1. Reproductive behavior in the squid Sepioteuthis australis from South Australia: interactions on the spawning grounds.

    Science.gov (United States)

    Jantzen, Troy M; Havenhand, Jon N

    2003-06-01

    Squid behavior is synonymous with distinctive body patterns, postures, and movements that constitute a complex visual communication system. These communications are particularly obvious during reproduction. They are important for sexual selection and have been identified as a potential means of species differentiation. Here we present a detailed account of copulation, mating, and egg deposition behaviors from in situ observations of the squid Sepioteuthis australis from South Australia. We identified four mating types from 85 separate mating attempts: "Male-upturned mating" (64% of mating attempts); "Sneaker mating" (33%); "Male-parallel" (2%); and "Head-to-head" (1%). Intervals between successive egg deposition behaviors were clearly bimodal, with modes at 2.5 s and 70.0 s. Ninety-three percent of egg capsules contained 3 or 4 eggs (mean = 3.54), and each egg cluster contained between 218 and 1922 egg capsules (mean = 893.9). The reproductive behavior of S. australis from South Australia was different from that described for other cephalopod species. More importantly, comparison between these results and those for other populations of S. australis suggests that behavior may differ from one population to another.

  2. Genital morphology of the male South American fur seal (Arctocephalus australis and biological implications

    Directory of Open Access Journals (Sweden)

    Alex Sander D. Machado

    2012-12-01

    Full Text Available Male capacity for spreading genes to a great number of descendents and to determine population dynamics depend directly on the genital organs. Morphological studies in pinnipeds are scarce and the functional meaning of some characteristics has never been discussed. We hypothesized that Arctocephalus australis (A. australis shows morphophysiological adaptations in order to guarantee the perpetuation of the species in the unique annual mating season. Seven males, dead from natural causes, had their genital organs collected and fixed for morphological description. Some features differ from other described mammalian males and are closely related to the biology and reproductive cycle of this species, as the scrotal epidermis, absence of glandular portion in the ductus deferens and spermatogenic epithelium suggest a recrudescent testis period. The corona glandis exhibits a singular arrangement: its erectile border looks like a formation of petals and its association with the os penis gives a "lily-flower" form to this region. We propose the name margo petaliformis to this particular erectile border of the corona glandis because of its similarity to a flower corola. The male genital organs of A. australis show morphological features compatible with adaptation to environment requirements and reproductive efficiency.

  3. Generation and multiplication of plantlets from callus derived from Haplopappus gracilus (Nutt.) Gray and their karyotype analysis

    Science.gov (United States)

    Kann, R. P.; O'Connor, S. A.; Levine, H. G.; Krikorian, A. D.

    1991-01-01

    Unopened flower heads of Haplopappus gracilis (2n = 4) provided primary explants for callus production and subsequent induction of organized growth. Callus was initiated from small (3-5 mm in length) floral buds with benzylaminopurine (BAP) (44.4 micromoles; 10 mg/l) and naphthalene acetic acid (NAA) (0.54 micromole; 0.1 mg/l). Lowering the BAP level to 4.44 micromoles (1 mg/l) but maintaining the NAA level, gave rise to organized but highly compressed shoot growing points from an otherwise undifferentiated callus mass. Shoots selected from such cultures were maintainable and could be proliferated by growing 1-1.5-cm stem tip cuttings on Murashige and Skoog basal medium (solidified with agar) containing 0.444 micromole (0.1 mg/l) BAP and 0.054 micromole (0.01 mg/l) NAA. The stem tip multiplication rates obtainable by these means permit reliable strategies for shoot multiplication or production of rooted plantlets. Prolonged subculture and maintenance of shoots on growth regulator-free medium leads to in vitro flowering and greatly reduces rooting capacity. Karyotype analysis of chromosomes from root tip cells at metaphase and chromosome measurements show that karyologically uniform plantlets (based on chromosome number and morphology) can be obtained.

  4. Neubauer's plantlet method - an alternative procedure for evaluating the effectiveness of potassium based fertilizers in reducing radiocaesium from soil to plant

    International Nuclear Information System (INIS)

    Mocanu, N.; Cotarlea, M.; Boldor, O.

    1996-01-01

    Accidental releases of radiocaesium into the environment have necessitated the search for effective soil-based countermeasures to reduce its transfer along food-chains. As field experiments can be impractical and protracted to predict rapidly the effectiveness of chemical treatments of radioactive contaminated soils to reduce soil-to-plant transfer of radionuclides, laboratory experiments are a suitable alternative for rapid evaluation of the most appropriate countermeasure to apply under a range of different circumstances in the event of accidental radioactive contamination of agricultural lands. Taking into account these considerations our study focuses on the use of a laboratory experiment based on Neubauer's plantlet method to evaluate the effectiveness of potassic salt 30 % applied on two soil types (alluvial and brown-reddish forest type) contaminated with 137 Cs for reducing uptake of the radionuclide to wheat plants grown-up on these soils. The experimental results evidence diminished values for 137 Cs/K quotient in wheat plantlets grown-up on soils treated with potassic salt 30 %, compared to those registered for wheat plantlets grown-up on untreated soils. The diminished values of 137 Cs/K quotient result from the reduced uptake of 137 Cs to plantlets accompanied by the enhanced uptake of potassium. (author)

  5. Differentiation of Cuscuta chinensis and Cuscuta australis by HPLC-DAD-MS analysis and HPLC-UV quantitation.

    Science.gov (United States)

    He, Xianghui; Yang, Wenzhi; Ye, Min; Wang, Qing; Guo, Dean

    2011-11-01

    Cuscuta chinensis and Cuscuta australis, the two botanical sources of the Chinese herbal medicine Tu-Si-Zi, were distinguished from each other based on qualitative and quantitative chemical analysis. By HPLC‑DAD‑MS, a total of 36 compounds were characterized from these two Cuscuta species, including 14 flavonoids, 17 quinic acid derivatives, and 5 lignans. In addition, HPLC‑UV was applied to determine seven major compounds (6 flavonoids plus chlorogenic acid) in 27 batches of Tu-Si-Zi. The results revealed that the amounts of the three classes of compounds varied significantly between the species. C. australis contained more flavonoids but less quinic acid derivatives and lignans than C. chinensis. Particularly, the amounts of kaempferol and astragalin in C. australis were remarkably higher than in C. chinensis. This finding could be valuable for the quality control of Tu-Si-Zi. © Georg Thieme Verlag KG Stuttgart · New York.

  6. In Vitro Selection of Peanut Somatic Embryos on Medium Containing Culture Filtrate of Sclerotium rolfsii and Plantlet Regeneration

    Directory of Open Access Journals (Sweden)

    YUSNITA

    2005-06-01

    Full Text Available Attempts to identify somaclonal variants of peanut with resistance to Sclerotium stem rot disease due to infection of S. rolfsii were conducted. The objectives of this study were to develop in vitro selection method using culture filtrates of S. rolfsii, identify culture filtrate-insensitive somatic embryo (SE of peanut after in vitro selection and regenerate peanut R0 lines originated from culture filtrate-insensitive SE. To achieve these objectives, peanut embryogenic tissues were cultured on selective medium containing various concentrations of S. rolfsii culture filtrates and sublethal concentration of the filtrates. Medium containing sublethal level of S. rolfsii culture filtrates was used to identify culture filtrate-insensitive SE of peanut. Subsequently, the selected SEs were germinated, plantlets were regenerated and preliminary tested against S. rolfsii. Results of the experiments showed that addition of S. rolfsii culture filtrates into medium for inducing peanut somatic embryos drastically reduced their growth and proliferation. S. rolfsii culture filtrates at 10% concentration has significantly reduced the number of proliferated SE per explant. However, sublethal level was achieved at 30% of culture filtrates concentration. Responses of five peanut cultivars against 30% of culture filtrates were similar, indicating they were similar in their susceptibility against S. rolfsii. A number of culture filtrate-insensitive SE were identified after culturing 1500 clumps of embryogenic tissue of peanut cv. Kelinci for three consecutive passages on medium containing 30% of culture filtrates. Germination of selected SE and regeneration of plantlet from culture filtrate-insensitive SE resulted in 50 peanut R0 lines. These lines have been grown in the plastic house and produced normal seeds for further evaluation. Results of S. rolfsii inoculation indicated the existence of chimera for insensitivity against S. rolfsii.

  7. Biomimetic synthesis of antimicrobial silver nanoparticles using in vitro-propagated plantlets of a medicinally important endangered species: Phlomis bracteosa.

    Science.gov (United States)

    Anjum, Sumaira; Abbasi, Bilal Haider

    2016-01-01

    In vitro-derived cultures of plants offer a great potential for rapid biosynthesis of chemical-free antimicrobial silver nanoparticles (AgNPs) by enhancing their phytochemical reducing potential. Here, we developed an efficient protocol for in vitro micropropagation of a high-value endangered medicinal plant species, Phlomis bracteosa, in order to explore its biogenic potential in biomimetic synthesis of antimicrobial AgNPs. Murashige and Skoog medium supplemented with 2.0 mg/L thidiazuron was found to be more efficient in inducing optimum in vitro shoot regeneration (78%±4.09%), and 2.0 mg/L indole-3-butyric acid was used for maximum root induction (86%±4.457%). Antimicrobial AgNPs were successfully synthesized by using aqueous extract (rich in total phenolics and flavonoids content) of in vitro derived plantlets of P. bracteosa. Ultraviolet-visible spectroscopy of synthesized AgNPs showed characteristic surface plasmon band in the range of 420-429 nm. The crystallinity, size, and shape of the AgNPs were characterized by X-ray diffraction and scanning electron microscopy. Face-centered cubic AgNPs of almost uniform spherical size (22.41 nm) were synthesized within a short time (1 hour) at room temperature. Fourier-transform infrared spectroscopy revealed that the polyphenols were mainly responsible for reduction and capping of synthesized AgNPs. Energy dispersive X-ray analysis further endorsed the presence of elemental silver in synthesized AgNPs. These biosynthesized AgNPs displayed significantly higher bactericidal activity against multiple drug-resistant human pathogens. The present work highlighted the potent role of in vitro-derived plantlets of P. bracteosa for feasible biosynthesis of antimicrobial AgNPs, which can be used as nanomedicines in many biomedical applications.

  8. Assessment of clonal fidelity of Tylophora indica (Burm. f.) Merrill "in vitro" plantlets by ISSR molecular markers.

    Science.gov (United States)

    Sharma, Madan Mohan; Verma, Roop Narayan; Singh, Abhijeet; Batra, Amla

    2014-01-01

    Tylophora indica Burm F. Merrill. is widely used against various diseases owing to the presence an array of medicinally important secondary metabolites. Its stem is bitter, stomachic, stimulates bile secretion, enriches the blood and cures diseases like diabetes, fever, flatulence, hypertension, jaundice, leucorrhoea, urinary disease and upper respiratory tract infection. It is neglected for tissue culture work because of deciduous nature of climbing shrub, facing problems for micropropagation. Hence, in vitro regeneration of complete plantlets was done through indirect organogenesis in Tylophora indica. Calli were produced from in vivo leaves of T. indica on MS medium supplemented with 6-Benzylaminopurine (BAP: 2.0 mg l(-1)) and Indole-3-butyric acid (IBA: 0.5 mg l(-1)). The multiple shoots (12.00 ± 1.50) emerged and elongated on MS medium fortified with Thidiazuron (TDZ: 0.1 mg l(-1)). They were rooted on half strength MS medium having IBA (0.5 mg l(-1)) (7.75 ± 0.25) after 20 days of sub-culturing followed by hardening and acclimatization. During indirect regeneration of plants, chances of somaclonal variations may arise. These variations should be identified to produce true to type plants. Plantlets raised through tissue culture were used to validate the clonal fidelity through Inter simple sequence repeat markers (ISSR). Clonal fidelity is a major consideration in commercial micropropagation using in vitro tissue culture methods. During the study, total 71 clear and distinct bands were produced using 6 primers. The banding pattern of each primer was uniform and comparable to mother plant and showed about 93% homology using un-weighted pair group method with arithmetic averaging (UPGMA). ISSR analysis confirmed the genetic stability of in vitro raised plants.

  9. Assessment of genetic fidelity in Rauvolfia serpentina plantlets grown from synthetic (encapsulated) seeds following in vitro storage at 4 °C.

    Science.gov (United States)

    Faisal, Mohammad; Alatar, Abdulrahman A; Ahmad, Naseem; Anis, Mohammad; Hegazy, Ahmad K

    2012-05-03

    An efficient method was developed for plant regeneration and establishment from alginate encapsulated synthetic seeds of Rauvolfia serpentina. Synthetic seeds were produced using in vitro proliferated microshoots upon complexation of 3% sodium alginate prepared in Llyod and McCown woody plant medium (WPM) and 100 mM calcium chloride. Re-growth ability of encapsulated nodal segments was evaluated after storage at 4 °C for 0, 1, 2, 4, 6 and 8 weeks and compared with non-encapsulated buds. Effects of different media viz; Murashige and Skoog medium; Lloyd and McCown woody Plant medium, Gamborg’s B5 medium and Schenk and Hildebrandt medium was also investigated for conversion into plantlets. The maximum frequency of conversion into plantlets from encapsulated nodal segments stored at 4 °C for 4 weeks was achieved on woody plant medium supplement with 5.0 μM BA and 1.0 μM NAA. Rooting in plantlets was achieved in half-strength Murashige and Skoog liquid medium containing 0.5 μM indole-3-acetic acid (IAA) on filter paper bridges. Plantlets obtained from stored synseeds were hardened, established successfully ex vitro and were morphologically similar to each other as well as their mother plant. The genetic fidelity of Rauvolfia clones raised from synthetic seeds following four weeks of storage at 4 °C were assessed by using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. All the RAPD and ISSR profiles from generated plantlets were monomorphic and comparable to the mother plant, which confirms the genetic stability among the clones. This synseed protocol could be useful for establishing a particular system for conservation, short-term storage and production of genetically identical and stable plants before it is released for commercial purposes.

  10. Effect of two vesicular-arbuscular mycorrhizal fungi on the growth of micropropagated potato plantlets and on the extent of disease caused by Rhizoctonia solani.

    Science.gov (United States)

    Yao, M K; Tweddell, R J; Désilets, H

    2002-10-01

    Two micropropagated potato cultivars, Goldrush and LP89221, were inoculated into sowing trays with either Glomus etunicatum or G. intraradices in a greenhouse. After 2 weeks, plantlets were transplanted into pots and roots were challenged 7 days later with Rhizoctonia solani. At different times after R. solani infection, disease severity, mortality rate, root colonization levels, various growth parameters, and shoot mineral content were evaluated. In Goldrush, only inoculation with G. etunicatum led to a significant reduction in disease severity, ranging between 60.2% and 71.2%, on both shoot and crown. This decrease was not observed in LP89221. Compared with the control plantlets, inoculation of Goldrush with G. etunicatum or G. intraradices reduced significantly the mortality rate by 77% and 26%, respectively, whereas vesicular-arbuscular mycorrhizal (VAM) fungi did not significantly influence the mortality rate in LP89221. In Goldrush, inoculation with G. etunicatum significantly increased shoot fresh weight, root dry weight and the number of tubers produced per plant, whereas G. intraradices only significantly increased the number of tubers. Tuber and root fresh weights of both potato cultivars were significantly reduced by R. solani infection. However, R. solani-infected plantlets of both Goldrush and LP89221, inoculated with G. etunicatum, produced significantly greater tuber fresh weight than non-VAM plantlets. In R. solani-infected plantlets of Goldrush but not LP89221, G. etunicatum and G. intraradices increased root fresh weight by approximately 140.3% and 76.5%, respectively, compared with non-VAM plants. The potato cultivars Goldrush and LP89221 responded differently to VAM fungal inoculation and to R. solani infection in terms of shoot mineral content.

  11. Role of extrinsic arbuscular mycorrhizal fungi in heavy metal-contaminated wetlands with various soil moisture levels.

    Science.gov (United States)

    Zheng, S; Wang, C; Shen, Z; Quan, Y; Liu, X

    2015-01-01

    This study presents an efficient heavy metal (HM) control method in HM-contaminated wetlands with varied soil moisture levels through the introduction of extrinsic arbuscular mycorrhizal fungi (AMF) into natural wetland soil containing indigenous AMF species. A pot culture experiment was designed to determine the effect of two soil water contents (5-8% and 25-30%), five extrinsic AMF inoculants (Glomus mosseae, G. clarum, G. claroideum, G. etunicatum, and G. intraradices), and HM contamination on root colonization, plant growth, and element uptake of common reed (Phragmites australis (Cav.) Trin. ex Steudel) plantlets in wetland soils. This study showed the prevalence of mycorrhizae in the roots of all P. australis plantlets, regardless of extrinsic AMF inoculations, varied soil moisture or HM levels. It seems that different extrinsic AMF inoculations effectively lowered HM concentrations in the aboveground tissues of P. australis at two soil moisture levels. However, metal species, metal concentrations, and soil moisture should also be very important factors influencing the elemental uptake performance of plants in wetland ecosystems. Besides, the soil moisture level significantly influenced plant growth (including height, and shoot and root dry weight (DW)), and extrinsic AMF inoculations differently affected shoot DW.

  12. Use of gamma irradiation as a quarantine control method for Frankliniella australis (Morgan) (Thysanoptera: Thripidae)

    International Nuclear Information System (INIS)

    Araya, J.E.; Curkovic, T.; Cayo, A.

    2007-01-01

    The black flower thrips, Frankliniella australis (Morgan), a Chilean species, causes quarantine rejections of fresh fruits for export. Today, fumigation with CH3Br is efficient to avoid these rejections, but is questioned because it affects the ozone layer, irradiation of foods being an alternative. The effect of gamma irradiation was studied in the laboratory on F. australis adults, at dosages of 250, 500, 750, and 1000 Gy, followed by storage at 0-5 deg C to simulate shipping conditions. Mortality immediately after irradiation was low (1.5 to 22.3% at 250 and 1000 Gy, respectively), but increased an hour later (15.8, 33.4, 44.5, and 51.7% with 250, 500, 750, and 1000 Gy, respectively). In this evaluation, mortality with the greatest dosage was significantly larger than with 500 and 250 Gy, while results with 750 and 500 Gy were not different between them but different with the smallest dosage. At 24 hours, mortality with all dosages surpassed 91%, although only that at 1000 Gy was significantly greater than at 250 Gy. At day 4th (96 hours) there was at least 98.8% mortality for the irradiation treatments, with significant differences with the control, which then presented only 12.8% mortality. Using logit regression with results up to day 4, a probit 9 (LD99.9968) for adults was estimated at 188 Gy. This dosage is slightly less to those indicated in the literature for control of other thrips, which may be explained because of the combined use of cold storage, although more studies are necessary of this technology under commercial conditions to verify its applicability for quarantine control of F. australis. (author) [es

  13. Growth responses of Phragmites karka - a candidate for second generation biofuel from degraded saline lands

    Science.gov (United States)

    Zaheer Ahmed, Muhammad; Shoukat, Erum; Abideen, Zainul; Aziz, Irfan; Gulzar, Salman; Ajmal Khan, M.

    2017-04-01

    Global changes like rapidly increasing population, limited fresh water resources, increasing salinity and aridity are the major causes of land degradation. Increasing feed production for bioenergy through direct and indirect land use cause major threat to biodiversity besides competing with food resources. Growing halophytes on saline lands would provide alternate source of energy without compromising food and cash crop farming. Phragmites karkahas recently emerged as a potential bio-fuel crop, which maintains optimal growth at 100 mM NaCl with high ligno-cellulosic biomass. However, temporal and organ specific plant responses under salinity needs to be understood for effective management of degraded saline lands. This study was designed to investigate variation in growth, water relations, ion-flux, damage markers, soluble sugars, stomatal stoichiometry and photosynthetic responses of P. karka to short (0-7 days) and long (15-30 days) term exposure with 0 (control), 100 (moderate) and 300 (high) mM NaCl. A reduced shoot growth ( 45%) during earlier (within 7 days) phase was observed in 300 mM NaCl compared to control and moderate salinity. Reduced leaf elongation rate and leaf senescence from 7th day in 300 mM NaCl (and later in moderate salinity) correspond to increasing hydrogen peroxide and malondialdehyde contents. Leaf turgor loss represents the osmotic effect of NaCl at both concentrations, however turgor recovered completely in moderate salinity within a week. Plant appeared to use both organic solutes (soluble sugars) and ions (Na++K++Cl-) for osmotic adjustment along with improved water use efficiency under saline conditions. Turgor loss in high salinity (300 mM NaCl) was related to increased bulk elastic modulus and decreased hydraulic capacitance which ultimately resulted in low water potential. Leaf Na+ and Cl- accumulation increased earlier (from 7th day) in 300 mM NaCl and later in 100 mM. Higher ion sequestration in different organs was found in the

  14. Conditions for selective degradation of lignin by the fungus Ganoderma australis

    Energy Technology Data Exchange (ETDEWEB)

    Rios, S.; Eyzaguirre, J. (Universidad Catolica de Chile, Santiago (Chile). Lab. de Bioquimica)

    1992-08-01

    The white-rot fungus Ganoderma australis selectively degrades lignin in the ecosystem 'palo podrido'. Using conditions that simulate those of 'palo podrido' in the laboratory, it was found that low nitrogen content and low O{sub 2} tension stimulate the production of manganese peroxidase and lignin degradation, and depress cellulose degradation and cellulase production. The inverse is found at high nitrogen concentration and high O{sub 2} tension. This agrees with previous results indicating that low O{sub 2} tension and low nitrogen stimulate selective lignin degradation by this fungus. (orig.).

  15. Uptake of inorganic phosphorus by the aquatic plant Isoetes australis inhabiting oligotrophic vernal rock pools

    DEFF Research Database (Denmark)

    Christiansen, Nina Høj; Pulido, Cristina; Pedersen, Ole

    2017-01-01

    The submerged aquatic freshwater macrophyte Isoetes australis S. Williams grows in rock pools situated in south-western Australia, an environment where dissolved inorganic phosphorus (Pi) availability possibly limits growth. In contrast to the two coexisting aquatic species, Glossostigma drummundii...... experiment revealed high amounts of Pi translocation internally in the plant which seemed to go from roots and oldest leaves to younger leaves. As a result of the high root to shoot ratio, high surface area, root uptake kinetics, and sediment Pi availability, roots accounted for 87% of plant Pi uptake...

  16. Isolation and amino acid sequence of a short-chain neurotoxin from an Australian elapid snake, Pseudechis australis.

    OpenAIRE

    Takasaki, C; Tamiya, N

    1985-01-01

    A short-chain neurotoxin Pseudechis australis a (toxin Pa a) was isolated from the venom of an Australian elapid snake Pseudechis australis (king brown snake) by sequential chromatography on CM-cellulose, Sephadex G-50 and CM-cellulose columns. Toxin Pa a has an LD50 (intravenous) value of 76 micrograms/kg body wt. in mice and consists of 62 amino acid residues. The amino acid sequence of Pa a shows considerable homology with those of short-chain neurotoxins of elapid snakes, especially of tr...

  17. Tabla de vida y parámetros poblacionales de Lacertinella australis (Insecta-Hemiptera-Fulgoromorpha

    Directory of Open Access Journals (Sweden)

    Maria F. ROSSI BATIZ

    2016-01-01

    Full Text Available Lacertinella australis ha sido capturada en 11 provincias de la Argentina y fue mencionada como una especie de interés fitosanitario por su potencial para vehiculizar fitopatógenos. Atendiendo al amplio rango de distribución, su potencial importancia fitosanitaria y el carácter invasivo de su principal planta hospedera, Cor - taderia spp., se evaluaron aspectos del ciclo de vida a través de una tabla de vida vertical bajo condiciones controladas en el laboratorio y se confeccionó una segunda tabla de vida sobre la base de un muestreo de una población natural. Se estimaron los atributos poblacionales: tasa reproductiva básica o tasa de reemplazo ( R 0 , tasa finita de crecimiento poblacional ( λ , capacidad de incremento poblacional ( r c , valor reproductivo ( V x , tiempo generacional de la cohorte ( T c y el promedio del tiempo de desarrollo por estadios. Se mencionan y comparan aspectos comportamentales y de la bionomía de L. australis compartidos con las dos especies de la tribu cuya biología se conoce: Saccharosydne procerus y Saccharosydne saccharivora.

  18. Effect of gamma irradiation on toxicity and immunogenicity of Androctonus australis hector venom

    International Nuclear Information System (INIS)

    Abib, L.; Laraba-Djebari, F.

    2003-01-01

    An investigation was made of the radiosensitivity of the toxic and immunological properties of Androctonus australis hector venom. This venom was irradiated with two doses of gamma rays (1 and 2 kGy) from a 60 Co source. The results showed that venom toxicity was abolished for the two radiation doses (1 and 2 kGy) with, respectively, 10 and 25 times its initial LD50 value. However, irradiated venoms were immunogenic, and the antibodies elicited by them were able to recognize the native venom by enzyme-linked immunosorbent assay. Antisera raised against these toxoids (1 and 2 kGy) had a higher neutralizing capacity and immunoreactivity against all components of native venom than did the antiserum produced against the native venom. The antiserum of rabbits immunized with 2-kGy-irradiated venom was more efficient than 1-kGy-irradiated toxoid antiserum. Indeed, in vivo protection assays showed that the mice immunized with 2-kGy-irradiated venom resisted lethal doses (i.p.) of A. australis hector venom. (author)

  19. The effect of sampling methods on the apparent constituents of ink from the squid Sepioteuthis australis.

    Science.gov (United States)

    Madaras, F; Gerber, J P; Peddie, F; Kokkinn, M J

    2010-11-01

    Results of experiments conducted on ink recovered from the squid Sepioteuthis australis indicate that there is no epinephrine or protein naturally present in the ink as it would be ejected in vivo. Protein content was effectively zero when ink was syringed from the duct end of the ink sac of freshly killed animals. By contrast, there were proteins in samples collected from dead specimens where ink was collected by a stripping method. From these samples, a single large molecular weight protein was identified as having tyrosinase activity. Digestion of syringed ink did not yield signs of melanin-bound proteins. Analysis of supernatants after centrifugation of squid ink consistently revealed the presence of DOPA, dopamine, and taurine, whereas epinephrine and nor-epinephrine were recorded from what was believed to be contaminated ink. Histological investigations of the ink sac revealed a compartmentalised glandular structure distal to the duct end. Closer observation of the glandular tissue showed that compartments increased in size as they matured and moved further into the lumen. It was concluded that the presence of epinephrine and tyrosinase (or a related protein) in the ink of S. australis could be attributed to rupturing of basal glandular compartments or contamination from other sources during the extraction process.

  20. Phytotoxic potential of celtis australis L. (family ulmaceae) against four crop species

    International Nuclear Information System (INIS)

    Ahmad, I.; Hussain, F.

    2014-01-01

    Bioassays were conducted to test the phytotoxic potential of Celtis australis against Trifolium alexandrinum, Brassica campestris, Triticum aestivum and Lactuca sativa under laboratory condition. Aqueous extracts from twigs and leaves were obtained by soaking 5 and 10g plant material in 100 ml distilled water for 24 and 48hr durations. Aqueous extracts significantly delayed/retarded the germination and reduced the plumule and radicle growth of all the four test species. Generally, extracts soaked for 48h especially 10 gm/100ml were inhibitory than 24h extracts of 5 or 10gm material. Extracts from twigs were inhibitory to germination of wheat while same extracts inhibited the plumule growth of B. campestris. Radicle growth of T. alexandrinum was inhibited more by twig extracts. Hot water extracts from twigs were less inhibitory than leaf extracts. Litter and mulch also significantly delayed the seed germination and retarded the overall growth of seedlings of all test species. The number and length of seminal roots of T. aestivum was suppressed by all aqueous extracts, added litter and mulch. The inhibitory response depended upon the test species, concentration, soaking duration and physiological parameters. The results suggested that Celtis australis has strong phytotoxic potential. (author)

  1. Assessment of the antiproliferative and antigenotoxic activity and phytochemical screening of aqueous extracts of Sambucus australis Cham. & Schltdl. (ADOXACEAE).

    Science.gov (United States)

    Tedesco, Marília; Kuhn, Andrielle W; Frescura, Viviane Dal-Souto; Boligon, Aline A; Athayde, Margareth L; Tedesco, Solange B; Silva, Antonio C F DA

    2017-01-01

    The purpose of this study was to evaluate the antiproliferative and antigenotoxic activity of Sambucus australis Cham. & Schltdl. aqueous extracts on the cell cycle of Allium cepa L. as well as determine the phenolic compounds in such extracts. S. australis inflorescences and leaves of two accessions were used for aqueous extract preparation at concentrations: 0.003 g/ml and 0.012 g/ml. A. cepa bulbs were rooted in distilled water and, subsequently, placed in treatments for 24 hours. Rootlets were collected and fixed in modified Carnoy's solution for 24 hours and kept. The squash technique was performed for slide preparation. Root tips were smashed and stained with 2% acetic orcein, and a total of 4000 cells per treatment were analyzed. The phenolic compounds were determined using high-performance liquid chromatography and data was analyzed using the Scott-Knott test. The results show that S. australis aqueous extracts have antiproliferative potential. Besides, the extracts prepared from S. australis leaves of both accessions at a concentration of 0.012 g/ml have shown antigenotoxic activity. The phytochemical analysis allowed us to determine the presence of flavonoids and phenolic acids, of which kaempferol and chrologenic acid were the most predominant compounds in the extracts from the inflorescences and leaves, respectively.

  2. Crescimento de mudas micropropagadas de bananeira micorrizadas em diferentes recipientes Growth of mycorrhizal micropropagated banana plantlets in different recipients

    Directory of Open Access Journals (Sweden)

    Patrícia Lopes Leal

    2005-04-01

    Full Text Available Realizou-se um experimento em casa de vegetação, com o objetivo de avaliar a utilização de diferentes recipientes e a eficiência da inoculação com fungo micorrízico arbuscular (FMA, Glomus clarum, no crescimento de mudas micropropagadas de bananeira. O delineamento experimental foi o de blocos casualizados, no esquema fatorial 2x2, sendo 2 tratamentos microbiológicos: Glomus clarum e controle; e 2 recipientes: blocos prensados e tubetes, com 6 repetições. O substrato utilizado para a confecção dos blocos e para o enchimento dos tubetes foi constituído por uma mistura de materiais orgânicos (bagaço de cana + torta de filtro de usina açucareira e vermiculita. Mudas de bananeira produzidas em blocos prensados e inoculadas com o FMA apresentaram melhores resultados quando comparadas com as produzidas nos tubetes, com incrementos na altura, na produção de matéria seca da parte aérea e no acúmulo de N, P e K de 90%, 829%, 2774%, 249% e 403%, respectivamente.An experiment under greenhouse was carried out in order to evaluate different recipients and the inoculation with arbuscular mycorrhizal fungus (AMF, Glomus clarum, on the growth of micropropagated banana plantlets. A random block design in a factorial scheme 2x2 (two microbiological treatments: Glomus clarum and control; two types of recipients: pressed blocks and plastic tubes, with six repetitions, was used. The substrate utilized to construct the pressed blocks and to fill the tubes was constituted by a mixture of organic residue (sugarcane bagasse and filter cake and vermiculite. Banana plantlets produced in pressed blocks and inoculated with AMF let to better results than their counterparts grown in tubes, increasing the height, shoot dry weight, contents of N, P and K in 90%, 829%, 2774%, 249% and 403%, respectively.

  3. Silver Nitrate and Different Culture Vessels Influence High Frequency Microrhizome Induction In Vitro and Enhancement Growth of Turmeric Plantlet During Ex Vitro Acclimatization

    Directory of Open Access Journals (Sweden)

    Dikash Singh THINGBAIJAM

    2012-11-01

    Full Text Available Eleven cultivars of C. longa var. Lakadong were collected from Manipur having different topography. Curcumin content in different cultivars has been analyzed by using UV-Visible Spectrophotometer (100 Bio-Carry Spectrophotometer. The curcuminoids content were analyzed and quantified for identification of best quality cultivar. Thoubal Cultivar with highest curcumin content (9.44% was subjected for tissue culture technique using different culture vessels and silver nitrate for rapid multiplication and scaling up of microrhizome production. High multiplication rate of 27.400.47 were obtained in Murashige and Skoogs medium supplemented with 3% sucrose + 1 mg L-1 ?-napthalene acetic acid, 4 mg L-1 6-benzyl-amino-purine and 11 ?M silver nitrate. Effect of different culture vessels and silver nitrate were studied for microrhizome and multiple shoots formation. Relatively higher rate of shoots along with microrhizome (17.50.32 can be seen in Growtek which was grown without any plant growth regulator. Growtek was used for scaling up of microrhizome production in vitro and utmost microrhizome was produced in liquid Murashige and Skoogs medium supplemented with 8% sucrose, 1 mg L-1 ?-napthalene acetic acid, 4 mg L-1 6-benzyl-amino-purine and 11 ?M silver nitrate (36.250.27. Addition of silver nitrate in the medium resulted in improvement of microrhizome induction in vitro. Higher concentration of silver nitrate (33, 44, 66, 88 ?M negatively affected the microrhizome and shoot multiplication and shows inhibition of tissue response completely. Analysis of in vitro derived plantlets during acclimatization shows that the exogenous applied of silver nitrate shows superior growth as compared to control. 90-95% of plantlets with and 75-80% plantlets without silver nitrate treatment were successfully established under ex vitro acclimatization. The protocol could be utilized for large scale production of true-to-type plantlets and as alternative method to step

  4. The effects of varying concentrations of growth regulators benzyladenine and naphthalene acetic acid and gamma irradiation on the gross plantlet morphology of Garcinia mangostana L

    International Nuclear Information System (INIS)

    Bonsol, Ma. Fatima Nona M.; Lasiste, Jade Marie Edenvirg F.; Quinio, Ma. Franchesca S.

    2004-02-01

    Mangosteen (Garcinia mangostana L.) is regarded as one of the best flavored fruits in the world. Though this fruit yields high income and has great potential as an export, productivity in the Philippines is still limited due to its slow and difficult conventional propagation. To increase the plants' productivity, growth regulators can be added, the seeds can be exposed to irradiation to cause genetic mutations, and micropropagation techniques can be used. This study seeks to determine the effects of these three variables on the general morphological characteristics of the plantlets grown both in vitro and in vivo. The first set of mangosteen seeds was inoculated and grown in Murashige and Skoog (MS) basal culture medium with different treatment combinations of growth regulators, naphthalene acetic acid (NAA) and benzyladenine (BA). Treatment concentrations ranged from 0 parts per million (ppm) to 6 ppm. The treatment that best induced stem, leaf and root formation, and produced plantlets with long stems and roots was MS with 6 ppm NAA combined with 6 ppm BA. A second set of seeds was then exposed to varying levels of gamma radiation and propagated in vivo and in vitro. For in vivo, it was observed that the length of stem of the plantlet decreases with increasing radiation dose; length of leaves of the mangosteen plantlets was best noted in seeds exposed to 10 Gy gamma rays. In in vitro, the two treatment concentrations that best helped seeds to develop callus, which were 0 ppm BA with 4 pmm NAA and 4 ppm BA with 6 ppm NAA, were added to MS basal medium. Seeds exposed to 5 Gy and grown in basal medium with 0 ppm BA and 4 ppm NAA were observed to have developed the most callus. (Author)

  5. Effect of cadmium on phenolic compounds, antioxidant enzyme activity and oxidative stress in blueberry (Vaccinium corymbosum L.) plantlets grown in vitro.

    Science.gov (United States)

    Manquián-Cerda, K; Escudey, M; Zúñiga, G; Arancibia-Miranda, N; Molina, M; Cruces, E

    2016-11-01

    Cadmium (Cd(2+)) can affect plant growth due to its mobility and toxicity. We evaluated the effects of Cd(2+) on the production of phenolic compounds and antioxidant response of Vaccinium corymbosum L. Plantlets were exposed to Cd(2+) at 50 and 100µM for 7, 14 and 21 days. Accumulation of malondialdehyde (MDA), hydrogen peroxide (H2O2) and the antioxidant enzyme SOD was determined. The profile of phenolic compounds was evaluated using LC-MS. The antioxidant activity was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the ferric reducing antioxidant power test (FRAP). Cd(2+) increased the content of MDA, with the highest increase at 14 days. The presence of Cd(2+) resulted in changes in phenolic compounds. The main phenolic compound found in blueberry plantlets was chlorogenic acid, whose abundance increased with the addition of Cd(2+) to the medium. The changes in the composition of phenolic compounds showed a positive correlation with the antioxidant activity measured using FRAP. Our results suggest that blueberry plantlets produced phenolic compounds with reducing capacity as a selective mechanism triggered by the highest activity of Cd(2+). Copyright © 2016 Elsevier Inc. All rights reserved.

  6. Biosynthesis of phenolic compounds and water soluble vitamins in culantro (Eryngium foetidum L. plantlets as affected by low doses of gamma irradiation

    Directory of Open Access Journals (Sweden)

    Amina Abd El-Hamid ALY

    2010-11-01

    Full Text Available Explants obtained from in-vitro propagated plantlets of Culantro (Eryngium foetidum L. were exposed to four dose levels of γ-irradiation (0.0, 10.0, 20.0 and 40.0 Gy to investigate the biosynthesis of phenolic compounds and water soluble vitamins in Culantro fresh plantlets. Among six identified phenolic compounds, the content of p-cumaric acid was the highest in the extracts, followed by caffeic acid, coumarin, benzoic acid, salicylic acid and apigenin. Significant increases were observed at dose 40.0 Gy (61.66 mg/g d.w. for flavonoids, 18.02 mg/g d.w, for flavonone and 5.06 mg/g d.w for anthocyanin compared to control. On the other hand, the flavonols were decreased by increasing the irradiation dose. Vitamin C was increased in irradiated samples and this increase was in correlation with irradiation dose level. Thiamin, riboflavin and nicotinic acid were enhanced by the applied dose level 10 Gy. In addition, folic acid was enhanced by the dose levels 20 and 40 Gy and not detected for the control and 10 Gy treatments. Meanwhile, pyridoxine was decreased by increasing the irradiation dose level. The results obtained suggested that both low doses of γ-irradiation and tissue culture technique could be used to produce plantlets with high amount of phenolic compounds and water soluble vitamins.

  7. LONG-TERM PRECONDITIONING OF PLANTLETS: A PRACTICAL METHOD FOR ENHANCING SURVIVAL OF PINEAPPLE (Ananas comosus Merr.) SHOOT TIPS CRYOPRESERVED USING VITRIFICATION.

    Science.gov (United States)

    Hu, W H; Liu, S F; Liaw, S I

    2015-01-01

    The purpose of this study was to develop an efficient cryopreservation protocol for pineapple (Ananas comosus Merr.) shoot tips. The optimal state of pineapple plantlets was investigated by using sucrose preconditioning to enhance survival after cryostorage. To achieve a suitable state of plantlets before cryopreservation, 0.2 M to 0.4 M sucrose concentrations combined with short- (0-7 days), medium- (15-30 days), and long-term (75-150 days) preconditioning periods were compared. The highest survival (100 %) was achieved using the following procedure: intact plantlets underwent long-term preconditioning with 0.2 M sucrose for 135 days, dissected shoot tips were treated with a loading solution containing 2.0 M glycerol + 0.4 M sucrose for 60 min at 25 degree and the shoot tips were dehydrated in PVS2 for 2h at 0 degree C before being plunged in liquid nitrogen. Rewarming was conducted in a water-bath for 30 s at 40 degree C and PVS2 was replaced with a 1.2 M sucrose solution for 30 min at 25 degree C. The shoot tips were transferred on semisolid medium and left in the dark for 1 week, then in dim light for 3 weeks.

  8. RAPD and ISSR based evaluation of genetic stability of micropropagated plantlets of Morus alba L. variety S-1

    Science.gov (United States)

    Saha, Soumen; Adhikari, Sinchan; Dey, Tulsi; Ghosh, Parthadeb

    2015-01-01

    Plant regeneration through rapid in vitro clonal propagation of nodal explants of Morus alba L. variety S-1 was established along with genetic stability analysis of regenerates. Axillary shoot bud proliferation was achieved on Murashige and Skoog (MS) medium in various culture regimes. Highest number of shoots (5.62 ± 0.01), with average length 4.19 ± 0.01 cm, was initially achieved with medium containing 0.5 mg/l N6-benzyladenine (BA) and 3% sucrose. Repeated subculturing of newly formed nodal parts after each harvest up to sixth passage, yielded highest number of shoots (about 32.27) per explants was obtained after fourth passage. Rooting of shoots occurred on 1/2 MS medium supplemented with 1.0 mg/1 Indole-3-butyric acid (IBA). About 90% (89.16) of the plantlets transferred to the mixture of sand:soil:organic manure (2:2:1) in small plastic pots acclimatized successfully. Genetic stability of the discussed protocol was confirmed by two DNA-based fingerprinting techniques i.e. RAPD (random amplified polymorphic DNA) and ISSR (inter-simple sequence repeat). This protocol can be used for commercial propagation and for future genetic improvement studies. PMID:26693403

  9. Structural Interaction Between GFP-Labeled Diazotrophic Endophytic Bacterium Herbaspirillum seropedicae RAM10 and Pineapple Plantlets 'VitóRia'.

    Science.gov (United States)

    Estrela Borges Baldotto, Lílian; Lopes Olivares, Fábio; Bressan-Smith, Ricardo

    2011-01-01

    The events involved in the structural interaction between the diazotrophic endophytic bacterium Herbaspirillum seropedicae, strain RAM10, labeled with green fluorescent protein, and pineapple plantlets 'Vitória' were evaluated by means of bright-field and fluorescence microscopy, combined with scanning electron microscopy for 28 days after inoculation. After 6 hours of inoculation, H. seropedicae was already adhered to the roots, colonizing mainly root hair surface and bases, followed by epidermal cell wall junctions. Bacteria adherence in the initial periods occurred mainly in the form of solitary cells and small aggregates with pleomorphic cells. Bacteria infection of root tissue occurred through the cavities caused by the disruption of epidermal cells during the emergence of lateral roots and the endophytic establishment by the colonization of intercellular spaces of the cortical parenchyma. Moreover, within 1 day after inoculation the bacteria were colonizing the shoots. In this region, the preferred sites of epiphytic colonization were epidermal cell wall junctions, peltate scutiform trichomes and non-glandular trichomes. Subsequently, the bacteria occupied the outer periclinal walls of epidermal cells and stomata. The penetration into the shoot occurred passively through stoma aperture followed by the endophytic establishment on the substomatal chambers and spread to the intercellular spaces of spongy chlorenchyma. After 21 days of inoculation, bacterial biofilm were seen at the root hair base and on epidermal cell wall surface of root and leaf, also confirming the epiphytic nature of H. seropedicae.

  10. Structural interaction between GFP-labeled diazotrophic endophytic bacterium Herbaspirillum seropedicae RAM10 and pineapple plantlets 'Vitória'

    Directory of Open Access Journals (Sweden)

    Lílian Estrela Borges Baldotto

    2011-03-01

    Full Text Available The events involved in the structural interaction between the diazotrophic endophytic bacterium Herbaspirillum seropedicae, strain RAM10, labeled with green fluorescent protein, and pineapple plantlets 'Vitória' were evaluated by means of bright-field and fluorescence microscopy, combined with scanning electron microscopy for 28 days after inoculation. After 6 hours of inoculation, H. seropedicae was already adhered to the roots, colonizing mainly root hair surface and bases, followed by epidermal cell wall junctions. Bacteria adherence in the initial periods occurred mainly in the form of solitary cells and small aggregates with pleomorphic cells. Bacteria infection of root tissue occurred through the cavities caused by the disruption of epidermal cells during the emergence of lateral roots and the endophytic establishment by the colonization of intercellular spaces of the cortical parenchyma. Moreover, within 1 day after inoculation the bacteria were colonizing the shoots. In this region, the preferred sites of epiphytic colonization were epidermal cell wall junctions, peltate scutiform trichomes and non-glandular trichomes. Subsequently, the bacteria occupied the outer periclinal walls of epidermal cells and stomata. The penetration into the shoot occurred passively through stoma aperture followed by the endophytic establishment on the substomatal chambers and spread to the intercellular spaces of spongy chlorenchyma. After 21 days of inoculation, bacterial biofilm were seen at the root hair base and on epidermal cell wall surface of root and leaf, also confirming the epiphytic nature of H. seropedicae.

  11. Cryopreservation of chayote (Sechium edule JACQ. SW.) zygotic embryos and shoot-tips from in vitro plantlets.

    Science.gov (United States)

    Abdelnour-Esquivel, Ana; Engelmann, Florent

    2002-01-01

    This paper presents the development of cryopreservation protocols for zygotic embryos and apices of chayote (Sechium edule Jacq. Sw.), a tropical plant species with recalcitrant seeds. Zygotic embryos of two cultivars, Ccocro negro (CN) and Claudio (Cl) could withstand cryopreservation, with survival percentages of 10 and 30 %, after desiccation to 23 and 19 % moisture content (fresh weight basis), respectively. Apices sampled on in vitro plantlets of cultivars Cl, 13 and JM were successfully cryopreserved using a vitrification technique. Optimal conditions included the culture of mother-plants for 22 days on medium containing 0.3 M sucrose, culture of excised apices on the same medium for 1 day, loading of apices for 20 min with 2M glycerol + 0.4M glycerol, treatment with a series of diluted PVS2 solution (60 % PVS2 followed by 80 % PVS2 solution for 15 min (cultivar Cocoro Blanco [CB]) or 30 min (cultivars CN and Cl) at each concentration), rapid freezing and thawing, washing of shoot-tips with a 1.2 M sucrose solution, followed by recovery on media with progressively decreasing sucrose concentrations until the standard concentration of 0.1 M was reached. The highest survival percentages achieved ranged between 17 and 38 %, depending on the cultivar.

  12. A sub-fossil kauri (Agathis australis) tree-ring chronology

    International Nuclear Information System (INIS)

    Bridge, M.C.; Ogden, J.

    1986-01-01

    A 491-year floating tree-ring chronology was constructed using sub-fossil bog kauris, Agathis australis, from a site near Huntly, North Island, New Zealand. The chronology has been approximately dated to within the period 3,500 B.P. to 3,000 B.P. by radiocarbon dating. This is the first sub-fossil New Zealand chronology, and shows the potential for the formation of a long chronology from the present back over several millenia. The applications of this chronology and its possible extensions include radiocarbon calibration for the Southern Hemisphere, and climatic reconstructions based on ring-widths. It will also assist interpretation of the history of the Waikato Valley over the last few millenia

  13. Metazoan parasites of Brama australis from southern Chile: a tool for stock discrimination?

    Science.gov (United States)

    Oliva, M E; Espinola, J F; Ñacari, L A

    2016-03-01

    The metazoan parasites of 403 specimens of the southern ray's bream Brama australis from three localities in southern Chile (Lebu 36° 70' S; 73° 40' W, Calbuco 41° 50' S; 73° 08' W and Punta Arenas 53° 10' S; 70° 50' W) were recorded. More than 23 400 parasite specimens belonging to 12 taxa were registered. Metazoan parasites were dominated by the copepod Hatschekia conifera, constituting 97% of the total number of parasites; the larval cestode Hepatoxylon trichiuri was the second most important parasite (2·1% of the total number of parasites). The remaining 10 species constituted parasites. Parasitological evidence, based on univariate and multivariate analysis, does not support the existence of discrete stocks in the studied zone. © 2016 The Fisheries Society of the British Isles.

  14. Radio emission from pre-main-sequence stars in Corona Australis

    International Nuclear Information System (INIS)

    Brown, A.

    1987-01-01

    The central region of the Corona Australis molecular cloud surrounding the stars R and TY CrA has been studied using the VLA at 6 cm. Eleven radio sources are detected including five associated with pre-main-sequence objects. The most striking is associated with the near-IR source IRS 7 and shows a complex structure comprising two strong pointlike sources positioned either side of the deeply embedded IR source and two extended lobes of radio emission. The IRS 7 radio source appears to be similar to that associated with Lynds 1551 IRS 5 but has a considerably larger angular size. The other detected sources include the massive pre-main-sequence star TY CrA, the near-IR sources IRS 1 and IRS 5, and the Herbig-Haro object HH 101. The stars R and T CrA were not detected. 35 references

  15. DNA hybridization evidence for the Australasian affinity of the American marsupial Dromiciops australis.

    Science.gov (United States)

    Kirsch, J A; Dickerman, A W; Reig, O A; Springer, M S

    1991-01-01

    DNA hybridization was used to compare representatives of the major groups of marsupials and a eutherian outgroup. Because of the large genetic distances separating marsupial families, trees were calculated from normalized percentages of hybridization; thermal-melting statistics, however, gave identical topologies for the well-supported clades. The most notable results were the association of the only extant microbiotheriid, Dromiciops australis, an American marsupial, with the Australasian Diprotodontia, and of both together with the Dasyuridae. Estimates of the rate of divergence among marsupial genomes suggest that the Dromiciops-Diprotodontia split occurred approximately 50 million years ago, well after the establishment of the major clades of marsupials but before deep oceanic barriers prohibited dispersal among Australia, Antarctica, and South America. Because Dromiciops is nested within an Australasian group, it seems likely that dispersal from Australia accounts for its present distribution. Images PMID:1961710

  16. Marine water mites (Acari: Hydrachnidia: Pontarachnidae) from Taiwan, Korea and India, with the first description of the male of Pontarachna australis Smit, 2003

    Digital Repository Service at National Institute of Oceanography (India)

    Pesic, V.; Chatterjee, T.; Chan, B.K.K.; Ingole, B.S.

    he hitherto unknown male of the marine water mite Pontarachna australis Smit, 2003 (Acari: Hydrachnidia: Pontarachnidae) is described and recorded from Taiwan for the first time. Another marine water mite, Litarachna denhami (Lohmann, 1909...

  17. Non-linear growth in tree ferns, Dicksonia antarctica and Cyathea australis.

    Science.gov (United States)

    Blair, David P; Blanchard, Wade; Banks, Sam C; Lindenmayer, David B

    2017-01-01

    Tree ferns are an important structural component of forests in many countries. However, because their regeneration is often unrelated to major disturbances, their age is often difficult to determine. In addition, rates of growth may not be uniform, which further complicates attempts to determine their age. In this study, we measured 5 years of growth of Cyathea australis and Dicksonia antarctica after a large wildfire in 2009 in south-eastern Australia. We found growth rates of these two species were unaffected by aspect and elevation but slope had a minor effect with D. antarctica growing 0.3mm faster for each additional degree of slope. Geographic location influenced growth in both species by up to 12 - 14mm/yr. The most consistent factor influencing growth rate, however, was initial height at the time of the 2009 fire; a finding consistent in both species and all geographic locations. For both tree fern species, individuals that were taller at the commencement of the study had greater overall growth for the duration of the study. This effect did not decrease even among the tallest tree ferns in our study (up to 6 metres tall). Overall, Cyathea australis averaged 73 (± 22)mm/year of growth (± 1SD), with the rate increasing 5mm/yr per metre of additional height. Dicksonia antarctica averaged 33 (± 13)mm/year, increasing by 6mm/yr/m. Growth rates dependent on initial height were unexpected and we discuss possible reasons for this finding. Variable growth rates also suggest that common age estimation methods of dividing height by average growth rate are likely to underestimate the age of short tree ferns, while overestimating the age of tall tree ferns, particularly if they have been subject to a fire.

  18. Morphology of the eye of the southern right whales (Eubalaena australis).

    Science.gov (United States)

    Buono, Mónica R; Fernández, Marta S; Herrera, Yanina

    2012-02-01

    Recently, there has been a growing interest in the anatomy and optics of the visual system of cetaceans. However, much of the new information has been focused on odontocetes, and relatively little is known about the visual anatomy of baleen whales. The aim of this study was describe the eye anatomy of the southern right whale (Eubalaena australis). Eye samples were collected from 26 calves, four adults with known body length, as well as two specimens of unknown body length that had stranded near their nursery ground at Península Valdés, Argentina, over 6 years. We provide anatomical descriptions of the eyeball and extraocular structures, as well as quantitative data in the form of eyeball, corneal, scleral, and lens measurements. To explore the sensitivity of the eye to light, the f-number was estimated in one specimen. We found that the eyes of the calves differed from those of the adults in having less periorbital fat surrounding the eyeball. We also observed variations in the abundance of periorbital fat among the adult specimens. The regression analysis revealed a correlation between body length and eyeball size. By contrast, the dimensions of the cornea were only weakly correlated with body length. The estimated f-number suggests that the optical sensitivity of the Eubalaena australis eye is relatively low. However, caution had to be taken in interpreting f-number as a proxy of eye sensitivity because it depends on the lens size, which can be affected by the fixation methods used. Copyright © 2011 Wiley Periodicals, Inc.

  19. Characterization and structural analysis of a potent anticoagulant phospholipase A2 from Pseudechis australis snake venom.

    Science.gov (United States)

    Du, Qianyun Sharon; Trabi, Manuela; Richards, Renée Stirling; Mirtschin, Peter; Madaras, Frank; Nouwens, Amanda; Zhao, Kong-Nan; de Jersey, John; Lavin, Martin F; Guddat, Luke W; Masci, Paul P

    2016-03-01

    Pseudechis australis is one of the most venomous and lethal snakes in Australia. Numerous phospholipase A2 (PLA2) isoforms constitute a major portion of its venom, some of which have previously been shown to exhibit not only enzymatic, but also haemolytic, neurotoxic and anticoagulant activities. Here, we have purified a potent anticoagulant PLA2 (identified as PA11) from P. australis venom to investigate its phospholipase, anticoagulant, haemolytic and cytotoxic activities and shown that addition of 11 nM PA11 resulted in a doubling of the clotting time of recalcified whole blood. We have also demonstrated that PA11 has high PLA2 enzymatic activity (10.9 × 10(4) Units/mg), but low haemolytic activity (0.6% of red blood cells hydrolysed in the presence of 1 nM PA11). PA11 at a concentration lower than 600 nM is not cytotoxic towards human cultured cells. Chemical modification experiments using p-bromophenacyl bromide have provided evidence that the catalytic histidine of PA11 is critical for the anticoagulant activity of this PLA2. PA11 that was subjected to trypsin digestion without previous reduction and alkylation of the disulfide bonds maintained enzymatic and anticoagulant activity, suggesting that proteolysis alone cannot abolish these properties. Consistent with these results, administration of PA11 by gavage in a rabbit stasis thrombosis model increased the clotting time of recalcified citrated whole blood by a factor of four. These data suggest that PA11 has potential to be developed as an anticoagulant in a clinical setting. Copyright © 2015. Published by Elsevier Ltd.

  20. Pharmacokinetics and pharmacodynamics of the myotoxic venom of Pseudechis australis (mulga snake) in the anesthetised rat.

    Science.gov (United States)

    Hart, A J; Hodgson, W C; O'Leary, M; Isbister, G K

    2014-07-01

    Myotoxicity is a common clinical effect of snake envenoming and results from either local or systemic myotoxins in snake venoms. Although numerous myotoxins have been isolated from snake venoms, there has been limited study on the relationship between the time course of venom concentrations (pharmacokinetics) and the time course of muscle injury measured as a rise in creatine kinase (CK) (pharmacodynamics). The aim of this study was to develop an in vivo model of myotoxicity to investigate the time course of myotoxicity and the effect of antivenom. Anesthetised rats were administered Pseudechis australis (mulga snake) venom either through i.v., i.m. or s.d. route, including a range of doses (5-100 μg/kg). Serial blood samples were collected for measurement of venom using enzyme immunoassay and measurement of CK and creatinine. Antivenom was administered before, 1 and 6 h after venom administration to investigate its effect on muscle injury. Plots of venom and CK versus time were made and the area under the curve (AUC) was calculated. There was a significant dose-dependent increase in CK concentration after administration of P. australis venom, which was greatest for i.v. administration. Timed measurement of venom concentrations showed a rapid absorption through s.d. and i.m. routes and a delayed rise in CK concentrations following any route. Antivenom prevented myotoxicity shown by a decrease in the CK AUC, which was most effective if given earliest. There was a rise in creatinine following i.v. venom administration. The study shows the delayed relationship between venom absorption and the rise in CK, consistent with the delayed onset of myotoxicity in human envenoming. Antivenom prevented myotoxicity more effectively if given earlier.

  1. Retinal amino acid neurochemistry of the southern hemisphere lamprey, Geotria australis.

    Directory of Open Access Journals (Sweden)

    Lisa Nivison-Smith

    Full Text Available Lampreys are one of the two surviving groups of the agnathan (jawless stages in vertebrate evolution and are thus ideal candidates for elucidating the evolution of visual systems. This study investigated the retinal amino acid neurochemistry of the southern hemisphere lamprey Geotria australis during the downstream migration of the young, recently-metamorphosed juveniles to the sea and during the upstream migration of the fully-grown and sexually-maturing adults to their spawning areas. Glutamate and taurine were distributed throughout the retina, whilst GABA and glycine were confined to neurons of the inner retina matching patterns seen in most other vertebrates. Glutamine and aspartate immunoreactivity was closely matched to Müller cell morphology. Between the migratory phases, few differences were observed in the distribution of major neurotransmitters i.e. glutamate, GABA and glycine, but changes in amino acids associated with retinal metabolism i.e. glutamine and aspartate, were evident. Taurine immunoreactivity was mostly conserved between migrant stages, consistent with its role in primary cell functions such as osmoregulation. Further investigation of glutamate signalling using the probe agmatine (AGB to map cation channel permeability revealed entry of AGB into photoreceptors and horizontal cells followed by accumulation in inner retinal neurons. Similarities in AGB profiles between upstream and downstream migrant of G. australis confirmed the conservation of glutamate neurotransmission. Finally, calcium binding proteins, calbindin and calretinin were localized to the inner retina whilst recoverin was localized to photoreceptors. Overall, conservation of major amino acid neurotransmitters and calcium-associated proteins in the lamprey retina confirms these elements as essential features of the vertebrate visual system. On the other hand, metabolic elements of the retina such as neurotransmitter precursor amino acids and Müller cells

  2. Otolith shape analysis as a tool for stock identification of the southern blue whiting, Micromesistius australis

    Directory of Open Access Journals (Sweden)

    Javier Leguá

    2013-07-01

    Full Text Available The southern blue whiting, Micromesistius australis (Norman, 1937, is an important demersal resource associated with the slope and continental shelf of southern Chile, Argentina and the Malvinas/Falkland Islands. Recent studies have reported schools of adult fish from Atlantic waters migrating along the southern Chilean coast in mid-winter, moving northwards to spawn in August (47°-51°S, and then returning to Atlantic waters, presumably to feed. The migratory pattern suggests the presence of one or more stock units associated with the South American coast. In the present study, "otolith morphometry" is used to determine the stock structure of M. australis based on applications of basic size descriptors (SDs (area, perimeter and otolith size, shape indices (SIs (circularity, squareness, shape factor, roundness, ellipticity, and normalised elliptical Fourier descriptors (NEFDs. Samples were collected during the winter and spring of 2010, during the reproductive period, in the economic zone of southern Chile (36°-57°S, in the Pacific Ocean and around the Falkland Islands economic zone (50°-52°S in the Atlantic Ocean. Analyses were conducted to include the effects of size, sex and age. A stepwise canonical discriminant analysis showed that fish were successfully discriminated with SDs, SIs and NEFDs. In this analysis, 86.4% and 70.1% of the fish were correctly classified as belonging to the Atlantic and Pacific stocks, respectively. A multivariate analysis of variance showed that the mean values of the NEFDs, SDs, and SIs did not vary significantly between sexes within areas (P > 0.05, but varied significantly between the Pacific and Atlantic oceans (P < 0.05. These results highlighted that otolith shape analysis can be a useful tool to evaluate the potential level of mixing in feeding areas where both stocks, the Pacific and Atlantic units, are expected to co-occur.

  3. Non-linear growth in tree ferns, Dicksonia antarctica and Cyathea australis.

    Directory of Open Access Journals (Sweden)

    David P Blair

    Full Text Available Tree ferns are an important structural component of forests in many countries. However, because their regeneration is often unrelated to major disturbances, their age is often difficult to determine. In addition, rates of growth may not be uniform, which further complicates attempts to determine their age. In this study, we measured 5 years of growth of Cyathea australis and Dicksonia antarctica after a large wildfire in 2009 in south-eastern Australia. We found growth rates of these two species were unaffected by aspect and elevation but slope had a minor effect with D. antarctica growing 0.3mm faster for each additional degree of slope. Geographic location influenced growth in both species by up to 12 - 14mm/yr. The most consistent factor influencing growth rate, however, was initial height at the time of the 2009 fire; a finding consistent in both species and all geographic locations. For both tree fern species, individuals that were taller at the commencement of the study had greater overall growth for the duration of the study. This effect did not decrease even among the tallest tree ferns in our study (up to 6 metres tall. Overall, Cyathea australis averaged 73 (± 22mm/year of growth (± 1SD, with the rate increasing 5mm/yr per metre of additional height. Dicksonia antarctica averaged 33 (± 13mm/year, increasing by 6mm/yr/m. Growth rates dependent on initial height were unexpected and we discuss possible reasons for this finding. Variable growth rates also suggest that common age estimation methods of dividing height by average growth rate are likely to underestimate the age of short tree ferns, while overestimating the age of tall tree ferns, particularly if they have been subject to a fire.

  4. Human consumption of a vagrant South American Fur Seal Arctocephalus australis (Carnivora: Otariidae in Brazil

    Directory of Open Access Journals (Sweden)

    S. Siciliano

    2016-04-01

    Full Text Available The South American Fur Seal Arctocephalus australis is one of the most widely distributed South American otariid species. In Brazil, during austral winter months specimens of A. australis are regularly found along the Rio Grande do Sul and Santa Catarina coasts. Occasionally, vagrants have been recorded along the southeastern coast but rarely moves north of Rio de Janeiro, at 23°S. On 01 May 2015, fishermen noticed in the surf zone an unidentified large animal in Ilhéus, Bahia, northeastern Brazil. Severely wounded, it was still alive when first spotted. Numerous shark bites were observed in the rostrum and flippers, the peduncle was severely lacerated and the intestines were protruding. The specimen was identified as an adult of South American Fur Seal based on the combination of the characteristic coloration of the pelage, head shape, body size, the relatively large eye size, and mainly due to the pronounced three cusped teeth in post canines. Fishermen decided to butcher the carcass and share the meat among their companions. The present record, however, is of special concern as human consumption South American Fur Seal seems to be unusual in Brazil. Pieces of meat were shared among fishermen. In addition, small pieces of blubber were used as bait in the local shark long-line fishery. Consumption of an injured and presumably weak marine mammal carcass points to an increasing risk of contact between potentially harmful viruses, bacteria and fungi and humans. This note represents an alert to public health specialists and wildlife authorities in Brazil. It calls the urgency of an outreach campaign advertising to the potential risks of ingesting bushmeat either from land or aquatic sources. 

  5. Phragmites karka as a Biosorbent for the Removal of Mercury Metal Ions from Aqueous Solution: Effect of Modification

    Directory of Open Access Journals (Sweden)

    Muhammad Hamid Raza

    2015-01-01

    Full Text Available Batch scale studies for the adsorption potential of novel biosorbent Phragmites karka (Trin, in its natural and treated forms, were performed for removal of mercury ions from aqueous solution. The study was carried out at different parameters to obtain optimum conditions of pH, biosorbent dose, agitation speed, time of contact, temperature, and initial metal ion concentration. To analyze the suitability of the process and maximum amount of metal uptake, Dubinin-Radushkevich (D-R model, Freundlich isotherm, and Langmuir isotherm were applied. The values of qmax for natural and treated biosorbents were found at 1.79 and 2.27 mg/g, respectively. The optimum values of contact time and agitation speed were found at 50 min and 150 rpm for natural biosorbent whereas 40 min and 100 rpm for treated biosorbent, respectively. The optimum biosorption capacities were observed at pH 4 and temperature 313 K for both natural P. karka and treated P. karka. RL values indicate that comparatively treated P. karka was more feasible for mercury adsorption compared to natural P. karka. Both pseudo-first-order and pseudo-second-order kinetic models were applied and it was found that data fit best to the pseudo-second-order kinetic model. Thermodynamic studies indicate that adsorption process was spontaneous, feasible, and endothermic.

  6. Acclimatization and leaf anatomy of micropropagated fig plantlets Aclimatização e anatomia foliar de plântulas de figo micropropagadas

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    Chrystiane Fráguas Chirinéa

    2012-12-01

    Full Text Available The survival of micropropagated plants during and after acclimatization is a limiting process to plant establishment. There is little information on how the anatomy of vegetative organs of Ficus carica can be affected by culture conditions and acclimatization. The present research aimed to study the effects of time on culture medium and substrates during the acclimatization of fig tree plantlets produced in vitro, characterizing some leaf anatomy aspects of plantlets cultured in vitro and of fig trees produced in field. Plantlets previously multiplied in vitro were separated and transferred into Wood Plant Medium (WPM where they were kept for 0, 15, 30, 45 and 60 days. Different substrates were tested and studies on leaf anatomy were performed in order to compare among plantlets grown in vitro, plantlets under 20, 40 and 60 days of acclimatization, and field grown plants. Keeping plantlets for 30 days in WPM allowed better development in Plantmax during acclimatization. Field grown plants presented higher number of stomata, greater epicuticular wax thickness and greater leaf tissue production compared to in vitro ones. The leaf tissues of in vitro plantlets show little differentiation and have great stomata number compared with acclimatized plants, which reduce the number of stomata during the acclimatization process.A sobrevivência de plantas micropropagadas durante e após a aclimatização é um processo limitante para o estabelecimento de plantas. Poucas informações são encontradas de como a anatomia de órgãos vegetativos de Ficus carica pode ser afetada pelas condições de cultivo e aclimatização. O presente trabalho teve por objetivo estudar os efeitos do tempo em meio de cultura e substratos durante a aclimatização de plântulas de figueira produzidas in vitro e cultivadas no campo. Plântulas multiplicadas in vitro foram separadas e transferidas para meio WPM, onde foram mantidas por 0; 15; 30; 45 e 60 dias. Diferentes

  7. Impact Of Thermotherapy And Chlorothalonil On Plantlets Production Of Some Genotypes Of Cassava Manihot Esculenta Crantz Produce In Benin

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    J.A.

    2017-01-01

    Full Text Available Cassava Manihot esculenta is a starchy root plant of great economic importance in sub-Saharan Africa and particularly in Benin. Its production is confronted to virus diseases which cause a considerable losses of yield. This work aims to determine the impact of thermotherapy and chlorothalonil in the production of cassava material of plantation. Cuttings of four varieties RB89509 BEN86052 9102319 92B0057 are cultivated under two conditions of thermotherapy and a control under greenhouse during 4 weeks. These different conditions are a closed drying oven with 16 hours photoperiod at 40 C the day and 36C the night a drying oven Binder with photoperiod of 12 hours at 38C the day and 28C the night and the control carried out under the conditions of the greenhouse. The media used was Murashige and Skoog MS added with various amounts of chlorothalonil 0.6 gl and 2gl and control without chlorothalonil. Both techniques of thermotherapy eliminate the virus symptoms of cassava at the rate of 0 seedling infected in thermotherapy against 16 seedlings in natural condition. The technique of closed drying oven significantly favors the production of nodes at 5 level p0.000 and shoots p0.02 on the other hand Binder drying oven has no significant effect on the production of shoots p0.68. The chlorothalonil had a positive effect on in vitro infestations elimination of cassava p0.05 but influenced the growth and development of cassava explants by reducing of nodes production p0.01 without a lethal effect on the plantlets until the dose of 2gl.

  8. In vitro propagation and assessment of genetic stability of acclimated plantlets of Cornus alba L. using RAPD and ISSR markers.

    Science.gov (United States)

    Ilczuk, Agnieszka; Jacygrad, Ewelina

    2016-01-01

    Cornus alba L. (white dogwood) is an important ornamental shrub having a wide range of applications such as reforestation programs and soil retention systems. The vegetative propagation of dogwood by cuttings may be slow, difficult, and cultivar dependent; therefore, an improved micropropagation method was developed. Nodal stem segments of C. alba cultivars 'Aurea' and 'Elegantissima' were cultured on media enriched with six different sources of macronutrients. Media were supplemented with either N 6 -benzyladenine (BA) or thidiazuron (TDZ) in combination with 1-naphthaleneacetic acid (NAA). Regardless of the cultivar, the best shoot proliferation was observed on Lloyd and McCown medium (woody plant medium (WPM)) at pH 6.2, containing 1.0 mg L -1 BA, 0.1 mg L -1 NAA, and 20-30 g L -1 sucrose. Rooting of regenerated shoots was achieved by an in vitro method when different concentrations of NAA or indole-3-butyric acid (IBA) were tested. Microcuttings were rooted for 8 wk on medium enriched with 0.25 mg L -1 NAA and potted into P9 containers in the greenhouse. The final survival rate of the plants after 20 wk was 80% for 'Aurea' and 90% for 'Elegantissima'. Genetic stability of the micropropagated plants was confirmed by using two DNA-based molecular marker techniques. A total of 30 random amplified polymorphic DNA (RAPD) and 20 inter-simple sequence repeat (ISSR) primers resulted in 197-199 and 184-187 distinct and reproducible band classes, respectively, in 'Aurea' and 'Elegantissima' plantlets. All of the RAPD and ISSR profiles were monomorphic and comparable with the mother plant.

  9. Effect of ferrous chloride on biogas production and enzymatic activities during anaerobic fermentation of cow dung and Phragmites straw.

    Science.gov (United States)

    Zhang, Huayong; Tian, Yonglan; Wang, Lijun; Mi, Xueyue; Chai, Yang

    2016-06-01

    The effect of ferrous (added as FeCl2) on the anaerobic co-digestion of Phragmites straw and cow dung was studied by investigating the biogas properties, pH values, organic matter degradation (COD) and enzyme activities (cellulase, protease and dehydrogenase) at different stages of mesophilic fermentation. The results showed that Fe(2+) addition increased the cumulative biogas yields by 18.1 % by extending the peak period with high daily biogas yields. Meanwhile, the methane (CH4) contents in the Fe(2+) added groups were generally higher than the control group before the 15th day. The pH values were not significantly impacted by Fe(2+) concentrations during the fermentation process. The COD concentrations, cellulase, protease and dehydrogenase activities varied with the added Fe(2+) concentrations and the stages of the fermentation process. At the beginning stage of fermentation (4th day), Fe(2+) addition increased the biogas production by improving the cellulase and dehydrogenase activities which caused a decline in COD. At the peak stage of fermentation (8th day), Fe(2+) addition enhanced the cellulase and protease activities, and resulted in lower COD contents than the control group. When the biogas yields decreased again (13th day), the COD contents varied similar with the protease and dehydrogenase activities, whilst cellulase activities were not sensitive to Fe(2+) concentrations. At the end of fermentation (26th day), Fe(2+) addition decreased the cellulase activities, led to lower COD contents and finally resulted the lower biogas yields than the control group. Taking the whole fermentation process into account, the promoting effect of Fe(2+) addition on biogas yields was mainly attributed to the extension of the gas production peak stage and the improvement of cellulase activities.

  10. FITOREMEDIASI TUMBUHAN AIR KIAMBANG (Salvinia molesta PURUN TIKUS (Eleocharis dulcis DAN PERUPUK (Phragmites karka SEBAGAI ALTERNATIF PENGOLAHAN LIMBAH CAIR KARET

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    Norhidayah Norhidayah

    2016-10-01

    Full Text Available The purpose of this study 1 assess the ability of aquatic plants Salvinia molesta, Eleocharis dulcis and Phragmites karka in reducing pollutant effluent, Zinc, DO, temperature, pH, BOD, COD, TSS, Turbidity  and Ammonia (NH3 in the rubber industry wastewater, 2 compare the water potential of plants to absorb pollutants wastewater rubber; 3 assess the status of the marine environment of rubber wastewater ponds to three (3 water treatment plant, the heavy metals zinc (Zn in liquid waste rubber. The results of the study resulted in plant water Purun Rat able to reduce BOD by 64%, COD by 17% and Turbidity by 80%, water kiambang able to lower Zinc by 49% and TSS by 70%, water plant perupuk able to reduce ammonia (NH3 by 23%. Stable temperature and pH value in each pool plant installation, the results of research conducted in this value in the effluent water quality rubber has not met the quality standard of waste according to Government Regulation No. 8 of 2001 concerning Management of Water Quality and Water Pollution Control. The results of the study resulted in plant water Purun Rat able to reduce BOD5 by 64%, COD by 17% and Turbidity by 80%, water kiambang able to lower Zinc by 49% and TSS by 70%, water plant perupuk able to reduce ammonia (NH3 by 23%. Stable temperature and pH value in each pool plant installation, the results of research conducted in this value in the effluent water quality rubber has not met the quality standard of waste according to Government Regulation No. 8 of 2001 concerning Management of Water Quality and Water Pollution Control.

  11. Genital morphology of the male South American fur seal (Arctocephalus australis and biological implications Morfologia dos órgãos genitais do macho do Lobo marinho (Arctocephalus australis e implicações biológicas

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    Alex Sander D. Machado

    2012-12-01

    Full Text Available Male capacity for spreading genes to a great number of descendents and to determine population dynamics depend directly on the genital organs. Morphological studies in pinnipeds are scarce and the functional meaning of some characteristics has never been discussed. We hypothesized that Arctocephalus australis (A. australis shows morphophysiological adaptations in order to guarantee the perpetuation of the species in the unique annual mating season. Seven males, dead from natural causes, had their genital organs collected and fixed for morphological description. Some features differ from other described mammalian males and are closely related to the biology and reproductive cycle of this species, as the scrotal epidermis, absence of glandular portion in the ductus deferens and spermatogenic epithelium suggest a recrudescent testis period. The corona glandis exhibits a singular arrangement: its erectile border looks like a formation of petals and its association with the os penis gives a "lily-flower" form to this region. We propose the name margo petaliformis to this particular erectile border of the corona glandis because of its similarity to a flower corola. The male genital organs of A. australis show morphological features compatible with adaptation to environment requirements and reproductive efficiency.A capacidade do macho de espalhar seus genes a um grande número de descendentes e determinar a dinâmica populacional depende diretamente dos seus órgãos genitais. Estudos morfológicos em pinípedes são escassos e o significado funcional de algumas de suas características ecológicas ainda foi pouco discutido. Nossa hipótese é que Arctocephalus australis (A. australis apresenta adaptações morfofisiológicas em seus órgãos genitais capazes de interagir com o meio e garantir a perpetuação da espécie que apresenta apenas uma época de acasalamento que ocorre uma vez a cada ano. Sete A. australis machos, mortos recentes por causas

  12. Silver Nitrate and Different Culture Vessels Influence High Frequency Microrhizome Induction In Vitro and Enhancement Growth of Turmeric Plantlet During Ex Vitro Acclimatization

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    Dikash Singh THINGBAIJAM

    2012-11-01

    Full Text Available Eleven cultivars of C. longa var. Lakadong were collected from Manipur having different topography. Curcumin content in different cultivars has been analyzed by using UV-Visible Spectrophotometer (100 Bio-Carry Spectrophotometer. The curcuminoids content were analyzed and quantified for identification of best quality cultivar. Thoubal Cultivar with highest curcumin content (9.44% was subjected for tissue culture technique using different culture vessels and silver nitrate for rapid multiplication and scaling up of microrhizome production. High multiplication rate of 27.40�0.47 were obtained in Murashige and Skoog�s medium supplemented with 3% sucrose + 1 mg L-1 ?-napthalene acetic acid, 4 mg L-1 6-benzyl-amino-purine and 11 ?M silver nitrate. Effect of different culture vessels and silver nitrate were studied for microrhizome and multiple shoots formation. Relatively higher rate of shoots along with microrhizome (17.5�0.32 can be seen in Growtek which was grown without any plant growth regulator. Growtek was used for scaling up of microrhizome production in vitro and utmost microrhizome was produced in liquid Murashige and Skoog�s medium supplemented with 8% sucrose, 1 mg L-1 ?-napthalene acetic acid, 4 mg L-1 6-benzyl-amino-purine and 11 ?M silver nitrate (36.25�0.27. Addition of silver nitrate in the medium resulted in improvement of microrhizome induction in vitro. Higher concentration of silver nitrate (33, 44, 66, 88 ?M negatively affected the microrhizome and shoot multiplication and shows inhibition of tissue response completely. Analysis of in vitro derived plantlets during acclimatization shows that the exogenous applied of silver nitrate shows superior growth as compared to control. 90-95% of plantlets with and 75-80% plantlets without silver nitrate treatment were successfully established under ex vitro acclimatization. The protocol could be utilized for large scale production of true-to-type plantlets and as alternative

  13. THE BIOTECHNOLOGY OF EMBRYOGENIC CELL LINES OBTAINING AND PLANTLETS OF CONIFEROUS SPECIES IN SIBERIA IN CULTURE IN VITRO

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    Tretiakova I.

    2012-08-01

    Full Text Available Experiments of culturing the immature isolated embryos and megagamethophytes of Siberian coniferous species were carried out on different modified media: ½ LV medium for Pinus sibirica and Pinus pumila, MSG and AI media (patent № 2431651 for Larix sibirica and Larix gmelinii, DCR medium for Picea ajanensis. For induction of embryogenic callus every species needs the optimized medium supplemented with L-glutamine, casein hydrolysate, ascorbic acid and hormones with different concentrations and their different proportions. The active proliferation of embryonal masses is observed on the same medium with reduced concentration of cytokinins. The proliferation of embryonal masses was significantly improved when they were subcultured after dispersing in liquid medium. The somatic embryos from embryonal masses are matured on basal medium with ABA (60-120 mM and PEG. In spite of species specificity the embryogenesis of morphogenic structures had the same scheme: elongation and asymmetric division of somatic cells, formation of initial cells and embryonal tubes, development of globular, torpedo and bipolar somatic embryos, embryos maturation and germination. However, not all donor-plants of coniferous species can form the embryogenic cell lines and somatic embryos. The active development of embryonal masses and formation of somatic embryos are observed from zygotic embryo of hybrid seeds of P. sibirica and L. sibirica. The obtained embryogenic lines were characterized by different proliferative activity. During 10 months cultivation the value of embryonal masses in different lines was 140-570 g. The number of somatic embryos varies from 210 to 410 per 100 mg of callus fresh weight. Decreasing proliferation activity did not observed during 24-45 months cultivation. However, development of somatic embryos in long cultivated lines decreased. Maturation of somatic embryos and development of plantlets were established in L. sibirica and P. pumila 50

  14. Proteomic analysis of blue light-induced twining response in Cuscuta australis.

    Science.gov (United States)

    Li, Dongxiao; Wang, Liangjiang; Yang, Xiaopo; Zhang, Guoguang; Chen, Liang

    2010-01-01

    The parasitic plant Cuscuta australis (dodder) invades a variety of species by entwining the stem and leaves of a host and developing haustoria. The twining response prior to haustoria formation is regarded as the first sign for dodders to parasitize host plants, and thus has been the focus of studies on the host-parasite interaction. However, the molecular mechanism is still poorly understood. In the present work, we have investigated the different effects of blue and white light on the twining response, and identified a set of proteins that were differentially expressed in dodder seedlings using a proteomic approach. Approximately 1,800 protein spots were detected on each 2-D gel, and 47 spots with increased or decreased protein levels were selected and analyzed with MALDI-TOF-MS. Peptide mass fingerprints (PMFs) obtained for these spots were used for protein identification through cross-species database searches. The results suggest that the blue light-induced twining response in dodder seedlings may be mediated by proteins involved in light signal transduction, cell wall degradation, cell structure, and metabolism.

  15. A comparison of the impact of 'seagrass-friendly' boat mooring systems on Posidonia australis.

    Science.gov (United States)

    Demers, Marie-Claire A; Davis, Andrew R; Knott, Nathan A

    2013-02-01

    Permanent boat moorings have contributed to the decline of seagrasses worldwide, prompting the development of 'seagrass-friendly' moorings. We contrasted seagrass cover and density (predominantly Posidonia australis) in the vicinity of three mooring types and nearby reference areas lacking moorings in Jervis Bay, Australia. We examined two types of 'seagrass-friendly' mooring and a conventional 'swing' mooring. 'Swing' moorings produced significant seagrass scour, denuding patches of ~9 m radius. Seagrass-friendly 'cyclone' moorings produced extensive denuded patches (average radius of ~18 m). Seagrass-friendly 'screw' moorings, conversely, had similar seagrass cover to nearby reference areas. Our findings reinforce previous work highlighting the negative effects of 'swing' and 'cyclone' moorings. In contrast, the previously unstudied 'screw' moorings were highly effective. We conclude that regular maintenance of moorings and the monitoring of surrounding seagrass are required to ensure that 'seagrass-friendly' moorings are operating effectively. This is important, as following damage Posidonia will take many decades to recover. Copyright © 2012 Elsevier Ltd. All rights reserved.

  16. Time monitoring of radio jets and magnetospheres in the nearby young stellar cluster R Coronae Australis

    International Nuclear Information System (INIS)

    Liu, Hauyu Baobab; Takami, Michihiro; Yan, Chi-Hung; Karr, Jennifer; Chou, Mei-Yin; Ho, Paul T.-P.; Galván-Madrid, Roberto; Costigan, Gráinne; Manara, Carlo Felice; Forbrich, Jan; Rodríguez, Luis F.; Zhang, Qizhou

    2014-01-01

    We report Karl G. Jansky Very Large Array 8-10 GHz (λ = 3.0-3.7 cm) monitoring observations toward the young stellar object (YSO) cluster R Coronae Australis (R CrA), taken from 2012 March 15 to 2012 September 12. These observations were planned to measure the radio flux variabilities in timescales from 0.5 hr to several days, to tens of days, and up to ∼200 days. We found that among the YSOs detectable in individual epochs, in general, the most reddened objects in the Spitzer observations show the highest mean 3.5 cm Stokes I emission, and the lowest fractional variabilities on <200 day timescales. The brightest radio flux emitters in our observations are the two reddest sources IRS7W and IRS7E. In addition, by comparing our observations with observations taken from 1996 to 1998 and 2005, we found that the radio fluxes of these two sources have increased by a factor of ∼1.5. The mean 3.5 cm fluxes of the three Class I/II sources, IRSI, IRS2, and IRS6, appear to be correlated with their accretion rates derived by a previous near-infrared line survey. The weakly accreting Class I/II YSOs, or those in later evolutionary stages, present radio flux variability on <0.5 hr timescales. Some YSOs were detected only during occasional flaring events. The source R CrA went below our detection limit during a few fading events.

  17. Paper production from wild dogwood (Cornus australis L. and the effect of bark on paper properties

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    Ayhan Gençer

    2017-11-01

    Full Text Available Generally bark has a negative effect pulp and paper properties. In this study, paper pulp and hand sheets were produced from Wild dogwood (Cornus australis L. using Kraft method. The cooking have been different conditions, chip / solution ratio 1/5, cooking temperature 170±2 °C by taking constant. Kraft method with the Na2S/NaOH, 18/20, 18/15, 18/10, 18/5 performed. Samples were used with and without bark in order to identify the negative impacts of the bark on pulp and paper production. In addition, it has been investigated whether the time of reaching the maximum temperature of K2 cooking is reduced from 120 minutes to 90 minutes, and the time and energy saving can be made. For all of the mechanical properties that were measured and pulp yield, the bark had a negative effect. But, this effect had not significant on mechanical properties at 95% significant level. On the other hand the bark had a negative effect on brightness and positive effects on opacity. These effects had significant at 95% significant level.

  18. Effects of nutrients on interaction between the invasive bidens pilosa the parasitic cusuta australis

    International Nuclear Information System (INIS)

    Yang, B.; Li, J.; Yan, M.

    2015-01-01

    Parasitic plants have been identified as potential biological agents to control invasive plants. Understanding the interaction between invasive plants and their novel natural enemies is important for understanding mechanisms underlying plant invasion success and thus taking measures to control invasion. We conducted a factorial experiment to test the interactive effects of nutrient addition (low vs. high) and parasitism (with vs. without Cuscuta australis) on the growth of the invasive Bidens pilosa. Parasitism significantly decreased leaf, stem and root biomass of the host invasive plant, and nutrient addition increased leaf and stem biomass of the host. A synergistic effect of parasitism and nutrient addition was found on stem and leaf biomass of the hosts. Nutrient addition significantly increased vegetative biomass of the parasitic plant and caused a more deleterious effect on the invasive host. Reproductive biomass of the parasitic plant was significantly positively related with net photosynthetic rate, light-utilisation efficiency and apparent carboxylation efficiency. Vegetative biomass and total biomass of the parasitic plants were significantly positively related with specific leaf area and the relative chlorophyll content of the host plant. The deleterious effect of the parasite on the growth of the host plant was significantly positively correlated with vegetative biomass of the parasitic plant. Nutrient addition increased the negative effect of the parasitic plant on the invasive host, indicating that the parasitic plant is potentially a biological control agent for the invasive plant even in the context of changing global resources. (author)

  19. Veiling and Accretion Around the Young Binary Stars S and VV Corona Australis

    Science.gov (United States)

    Sullivan, Kendall; Prato, Lisa; Avilez, Ian

    2018-01-01

    S CrA and VV CrA are two young binary star systems with separations of 170 AU and 250 AU, respectively, in the southern star-forming region Corona Australis. The spectral types of the four stars in these two systems are similar, approximately K7 to M1, hence the stellar masses are also similar. The study of young stars just emerging from their natal cloud cores at the very limits of observability allows us to probe the extreme environments in which planet formation begins to occur. Stars in this early evolutionary stage can have circumstellar or circumbinary disks, and sometimes remnants of the envelopes which surrounded them during the protostellar stage. Envelopes accrete onto disks and disks in turn accrete onto the central stars, triggering elevated continuum emission, line emission, outflows, and stellar winds. This violent stage marks the onset of the epoch of planet formation. Using high-resolution near-infrared, H-band spectroscopy from the Keck II telescope using the NIRSPEC instrument over 4-6 epochs, we are probing the chaotic environment surrounding the four stars in these systems. We determine the spectral types for VV CrA A and B for the first time, and examine the variable veiling and emission occurring around each of these stars. This research was supported in part by NSF grants AST-1461200 and AST-1313399.

  20. Clinical Manifestations and Outcomes of Rickettsia australis Infection: A 15-Year Retrospective Study of Hospitalized Patients

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    Adam Stewart

    2017-06-01

    Full Text Available Queensland tick typhus (QTT; Rickettsia australis is an important cause of community-acquired acute febrile illness in eastern Australia. Cases of QTT were identified retrospectively from 2000 to 2015 at five sites in Northern Brisbane through a pathology database. Those included had a fourfold rise in spotted fever group (SFG-specific serology, a single SFG-specific serology ≥ 256 or SFG-specific serology ≥ 128 with a clinically consistent illness. Cases were excluded on the basis of clinical unlikelihood of QTT infection. Thirty-six cases were included. Fever was found in 34/36 (94% patients. Rash occurred in 83% of patients with maculopapular being the dominant morphology (70%. Thrombocytopenia, lymphopenia, and raised transaminases were common and occurred in 58%, 69%, and 89% of patients, respectively. Thirty-one of 36 (86% patients received antibiotic therapy (usually doxycycline and the time to correct antibiotic (from admission ranged from 3 to 120 h (mean 45.5 h. Four of 36 (11% required intensive care unit (ICU admission for severe sepsis and end-organ support. There were no deaths. QTT has a wide range of clinical and laboratory features. Early and appropriate antimicrobial therapy is important and may prevent severe disease. Further prospective studies are required to identify factors associated with severe infection and sepsis.

  1. Nutritional, antioxidative, and antimicrobial analysis of the Mediterranean hackberry (Celtis australis L.).

    Science.gov (United States)

    Ota, Ajda; Višnjevec, Ana Miklavčič; Vidrih, Rajko; Prgomet, Željko; Nečemer, Marijan; Hribar, Janez; Cimerman, Nina Gunde; Možina, Sonja Smole; Bučar-Miklavčič, Milena; Ulrih, Nataša Poklar

    2017-01-01

    Celtis australis is a deciduous tree commonly known as Mediterranean hackberry or the European nettle tree. The fruit of hackberry are seldom used for nutritional purposes. The nutritional and physicochemical properties of ripe hackberry fruit from Istria (Marasi village near Vrsar, Croatia) were determined, including water, total fiber, protein, vitamin, mineral, and phenolic contents. This analysis demonstrates that the hackberry fruit is a valuable source of dietary fiber, protein, and vitamins, and of pigments such as lutein, β -carotene, zeaxanthin, and tocopherols. The seasonal differences associated with the different growth stages for the element composition, total phenolic content, and phenolic profile were also determined for hackberry mesocarp and leaves. Water and ethanol extracts were prepared from mesocarp and leaves harvested at different growth stages and their phenolic profiles and antioxidant and antimicrobial activities were investigated. This study demonstrates that water and ethanol extracts of hackberry fruit and leaves collected at different growth stages contain epicatechin, gallic acid, vanillic acid, 3,4-dihydroxybenzaldehyde, delphinidin-3,5-di-O-glucoside, cyanidin-3,5-di-O-glucoside, and pelargonidin-3,5-di-O-glucoside. They also show some antimicrobial and antifungal activities. Further studies are needed to identify and define the active ingredients of these hackberry leaf ethanol extracts.

  2. Covalent structure of the insect toxin of the North African scorpion Androctonus australis Hector

    International Nuclear Information System (INIS)

    Darbon, H.; Kopeyan, C.; Rietschoten, J. van; Rochat, H.; Zlotkin, E.

    1982-01-01

    The complete covalent structure of the insect toxin purified from the venom of the North-African scorpion Androctonus australis Hector was described. Its amino acid sequence was established by phenylisothiocyanate degradation of several protein derivatives and proteolytic fragments in a liquid protein sequencer using either a ''protein'' or a ''peptide'' program. The position of the four disulfide bridges were deduced by analysis of proteolytic peptides before and after performic oxidation, and by partial labeling of the half cystine residues with [ 14 C]-iodoacetic acid and determining the specific radioactivities of the S-[ 14 C]-carboxymethylated phenylthiohydantoin cysteines. The sequences of the insect and mammal toxins from scorpions can be aligned with homology with the positions of seven half-cystine residues as registers. The mammal and insect toxins have three disulfide bridges at homologous positions. The fourth bridge is different in that Cys 12 in mammal toxin II is replaced by Cys 38 in the insect toxin. It is likely that the position of the disulfide bridges is the same for all scorpion neurotoxins active on mammals. We believe that the shift of one half-cystine residue in the insect toxin may induce a conformational change in the structure of the protein, which, in turn, may partially account for the total specificity of this toxin for insect nervous system. (author)

  3. Cellular and ultrastructural characterization of the grey-morph phenotype in southern right whales (Eubalaena australis).

    Science.gov (United States)

    Eroh, Guy D; Clayton, Fred C; Florell, Scott R; Cassidy, Pamela B; Chirife, Andrea; Marón, Carina F; Valenzuela, Luciano O; Campbell, Michael S; Seger, Jon; Rowntree, Victoria J; Leachman, Sancy A

    2017-01-01

    Southern right whales (SRWs, Eubalena australis) are polymorphic for an X-linked pigmentation pattern known as grey morphism. Most SRWs have completely black skin with white patches on their bellies and occasionally on their backs; these patches remain white as the whale ages. Grey morphs (previously referred to as partial albinos) appear mostly white at birth, with a splattering of rounded black marks; but as the whales age, the white skin gradually changes to a brownish grey color. The cellular and developmental bases of grey morphism are not understood. Here we describe cellular and ultrastructural features of grey-morph skin in relation to that of normal, wild-type skin. Melanocytes were identified histologically and counted, and melanosomes were measured using transmission electron microscopy. Grey-morph skin had fewer melanocytes when compared to wild-type skin, suggesting reduced melanocyte survival, migration, or proliferation in these whales. Grey-morph melanocytes had smaller melanosomes relative to wild-type skin, normal transport of melanosomes to surrounding keratinocytes, and normal localization of melanin granules above the keratinocyte nuclei. These findings indicate that SRW grey-morph pigmentation patterns are caused by reduced numbers of melanocytes in the skin, as well as by reduced amounts of melanin production and/or reduced sizes of mature melanosomes. Grey morphism is distinct from piebaldism and albinism found in other species, which are genetic pigmentation conditions resulting from the local absence of melanocytes, or the inability to synthesize melanin, respectively.

  4. The Composition and The Content of The Main Pigments on Dodders Plant Cuscuta australis R.Br. and Cassytha filiformis L.

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    Heriyanto

    2006-11-01

    Full Text Available Research on dodders plant Cuscuta australis R.Br. and Cassytha filiformis L was done to analyze their pigment composition and content. The pigment composition was analyzed by the use of thin layer chromatography (TLC method based on spot color and retardation factor. The water content was measured according to Sudarmadji et. al. The chlorophylls and carotenoid contents were calculated by Porra et. al. and Gross equation, respectively. Result showed that Cuscuta australis R.Br. (green yellowish and orange and Cassytha filiformis L. (green and brown reddish had similar pigment composition consist of carotene, pheophytin a, chlorophyll a, chlorophyll b and xanthophyll. The average of the chlorophyll content from the highest to the lowest one was Cassytha filiformis L. green followed by Cuscuta australis R.Br. green yellowish and orange, while the total chlorophyll of Cassytha filiformis L. brown reddish was relatively similar with other dodders plant. The average of the carotenoid content from the highest to the lowest was Cuscuta australis R.Br. orange followed by Cuscuta australis R.Br. green yellowish. The pigment content of Cassytha filiformis L. was relatively similar to Cassytha filiformis L. brown reddish.

  5. Comparative studies about the influence of salicylic and acetylsalicilic acid on content of assimilatory pigments in the primary leaves of wheat (Triticum aestivum plantlets

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    Cornelia PURCAREA

    2007-05-01

    Full Text Available Salicylic acid (SA and some of its derivates are phenolic compounds recently recognized as plant growth regulators involved in many physiological processes including photosynthesis. One of the important derivates of Salicylic Acid is the Acetylsalicylic Acid. In the present investigation we studied the influence of exogenous Acetylsalicylic and Salicylic acid with different concentrations on the assimilatory pigments contents of the primary leaves of wheat seedlings in comparison with the same parameters of the control lots which were treated with water. The wheat seedlings were soaked for 6 hours in 0.01mM; 0.1mM; 0.5mM and 1 mM SA or ASA solutions and in water for the control lot, germinated for 7 days on filter paper moistened with water. After that, we planted the plantlets in sand and sprayed their coleoptiles and primary leaves, each day for an additional 7 days, with water. In the 14th days of germination we determined the content of assimilatory pigments extracted with N,N-dimethylformamide (DMF. The results showed that exogenous 0.01 mM, 0.1mM, 0.5 mM or 1.0 mM SA solution treatments cause more significant increases in the assimilatory pigments contents in leaves of wheat plantlets than treatments with ASA solutions of the same concentrations do.

  6. Phylogeography of Australia's king brown snake (Pseudechis australis) reveals Pliocene divergence and Pleistocene dispersal of a top predator

    Science.gov (United States)

    Kuch, Ulrich; Keogh, J. Scott; Weigel, John; Smith, Laurie A.; Mebs, Dietrich

    2005-03-01

    King brown snakes or mulga snakes (Pseudechis australis) are the largest and among the most dangerous and wide-ranging venomous snakes in Australia and New Guinea. They occur in diverse habitats, are important predators, and exhibit considerable morphological variation. We infer the relationships and historical biogeography of P. australis based on phylogenetic analysis of 1,249 base pairs from the mitochondrial cytochrome b, NADH dehydrogenase subunit 4 and three adjacent tRNA genes using Bayesian, maximum-likelihood, and maximum-parsimony methods. All methods reveal deep phylogenetic structure with four strongly supported clades comprising snakes from New Guinea (I), localities all over Australia (II), the Kimberleys of Western Australia (III), and north-central Australia (IV), suggesting a much more ancient radiation than previously believed. This conclusion is robust to different molecular clock estimations indicating divergence in Pliocene or Late Miocene, after landbridge dispersal to New Guinea had occurred. While members of clades I, III and IV are medium-sized, slender snakes, those of clade II attain large sizes and a robust build, rendering them top predators in their ecosystems. Genetic differentiation within clade II is low and haplotype distribution largely incongruent with geography or colour morphs, suggesting Pleistocene dispersal and recent ecomorph evolution. Significant haplotype diversity exists in clades III and IV, implying that clade IV comprises two species. Members of clade II are broadly sympatric with members of both northern Australian clades. Thus, our data support the recognition of at least five species from within P. australis (auct.) under various criteria. We discuss biogeographical, ecological and medical implications of our findings.

  7. Toxicokinetic and toxicodynamic analyses of Androctonus australis hector venom in rats: Optimization of antivenom therapy

    International Nuclear Information System (INIS)

    Hammoudi-Triki, D.; Lefort, J.; Rougeot, C.; Robbe-Vincent, A.; Bon, C.; Laraba-Djebari, F.; Choumet, V.

    2007-01-01

    This paper reports the simultaneous determination of toxicokinetic and toxicodynamic properties of Androctonus australis hector venom, in the absence and presence of antivenom (F(ab') 2 and Fab), in envenomed rats. After subcutaneous injection of the venom, toxins showed a complete absorption phase from the site of injection associated with a distribution into a large extravascular compartment. The injection of Fab and F(ab') 2 induced the neutralization of venom antigens in the blood compartment, as well as the redistribution of venom components from the extravascular compartment to the blood compartment. Interestingly, F(ab') 2 and Fab showed distinct efficiencies depending on their route of injection. F(ab') 2 induced a faster venom neutralization and redistribution than Fab when injected intravenously. Fab was more effective than F(ab') 2 by the intramuscular route. The hemodynamic effects of Aah venom were further investigated. Changes in mean arterial pressure and heart rate were observed in parallel with an upper airway obstruction. Fab was more effective than F(ab') 2 for preventing early symptoms of envenomation, whatever their route of administration. Intraperitoneal injection of F(ab') 2 and Fab was similar for the prevention of the delayed symptoms, even after a late administration. Fab was more effective than F(ab') 2 in the inhibition of airway resistance, independent of the route and time of administration. These results show that the treatment for scorpion stings might be improved by the intravascular injection of a mixture of Fab and F(ab') 2 . If antivenom cannot be administered intravenously, Fab might be an alternative as they are more effective than F(ab') 2 when injected intramuscularly

  8. Comparison of mercury contamination in live and dead dolphins from a newly described species, Tursiops australis.

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    Alissa Monk

    Full Text Available Globally it is estimated that up to 37% of all marine mammals are at a risk of extinction, due in particular to human impacts, including coastal pollution. Dolphins are known to be at risk from anthropogenic contaminants due to their longevity and high trophic position. While it is known that beach-cast animals are often high in contaminants, it has not been possible to determine whether levels may also be high in live animals from the same populations. In this paper we quantitatively assess mercury contamination in the two main populations of a newly described dolphin species from south eastern Australia, Tursiops australis. This species appear to be limited to coastal waters in close proximity to a major urban centre, and as such is likely to be vulnerable to anthropogenic pollution. For the first time, we were able to compare blubber mercury concentrations from biopsy samples of live individuals and necropsies of beach-cast animals and show that beach-cast animals were highly contaminated with mercury, at almost three times the levels found in live animals. Levels in live animals were also high, and are attributable to chronic low dose exposure to mercury from the dolphin's diet. Measurable levels of mercury were found in a number of important prey fish species. This illustrates the potential for low dose toxins in the environment to pass through marine food webs and potentially contribute to marine mammal deaths. This study demonstrates the potential use of blubber from biopsy samples to make inferences about the health of dolphins exposed to mercury.

  9. Comparison of mercury contamination in live and dead dolphins from a newly described species, Tursiops australis.

    Science.gov (United States)

    Monk, Alissa; Charlton-Robb, Kate; Buddhadasa, Saman; Thompson, Ross M

    2014-01-01

    Globally it is estimated that up to 37% of all marine mammals are at a risk of extinction, due in particular to human impacts, including coastal pollution. Dolphins are known to be at risk from anthropogenic contaminants due to their longevity and high trophic position. While it is known that beach-cast animals are often high in contaminants, it has not been possible to determine whether levels may also be high in live animals from the same populations. In this paper we quantitatively assess mercury contamination in the two main populations of a newly described dolphin species from south eastern Australia, Tursiops australis. This species appear to be limited to coastal waters in close proximity to a major urban centre, and as such is likely to be vulnerable to anthropogenic pollution. For the first time, we were able to compare blubber mercury concentrations from biopsy samples of live individuals and necropsies of beach-cast animals and show that beach-cast animals were highly contaminated with mercury, at almost three times the levels found in live animals. Levels in live animals were also high, and are attributable to chronic low dose exposure to mercury from the dolphin's diet. Measurable levels of mercury were found in a number of important prey fish species. This illustrates the potential for low dose toxins in the environment to pass through marine food webs and potentially contribute to marine mammal deaths. This study demonstrates the potential use of blubber from biopsy samples to make inferences about the health of dolphins exposed to mercury.

  10. Comparison of Mercury Contamination in Live and Dead Dolphins from a Newly Described Species, Tursiops australis

    Science.gov (United States)

    Monk, Alissa; Charlton-Robb, Kate; Buddhadasa, Saman; Thompson, Ross M.

    2014-01-01

    Globally it is estimated that up to 37% of all marine mammals are at a risk of extinction, due in particular to human impacts, including coastal pollution. Dolphins are known to be at risk from anthropogenic contaminants due to their longevity and high trophic position. While it is known that beach-cast animals are often high in contaminants, it has not been possible to determine whether levels may also be high in live animals from the same populations. In this paper we quantitatively assess mercury contamination in the two main populations of a newly described dolphin species from south eastern Australia, Tursiops australis. This species appear to be limited to coastal waters in close proximity to a major urban centre, and as such is likely to be vulnerable to anthropogenic pollution. For the first time, we were able to compare blubber mercury concentrations from biopsy samples of live individuals and necropsies of beach-cast animals and show that beach-cast animals were highly contaminated with mercury, at almost three times the levels found in live animals. Levels in live animals were also high, and are attributable to chronic low dose exposure to mercury from the dolphin's diet. Measurable levels of mercury were found in a number of important prey fish species. This illustrates the potential for low dose toxins in the environment to pass through marine food webs and potentially contribute to marine mammal deaths. This study demonstrates the potential use of blubber from biopsy samples to make inferences about the health of dolphins exposed to mercury. PMID:25137255

  11. Against the odds: complete outcrossing in a monoecious clonal seagrass Posidonia australis (Posidoniaceae).

    Science.gov (United States)

    Sinclair, Elizabeth A; Gecan, Ilena; Krauss, Siegfried L; Kendrick, Gary A

    2014-06-01

    Seagrasses are marine, flowering plants with a hydrophilous pollination strategy. In these plants, successful mating requires dispersal of filamentous pollen grains through the water column to receptive stigmas. Approximately 40 % of seagrass species are monoecious, and therefore little pollen movement is required if inbreeding is tolerated. Outcrossing in these species is further impacted by clonality, which is variable, but can be extensive in large, dense meadows. Despite this, little is known about the interaction between clonal structure, genetic diversity and mating systems in hydrophilous taxa. Polymorphic microsatellite DNA markers were used to characterize genetic diversity, clonal structure, mating system and realized pollen dispersal in two meadows of the temperate, monoecious seagrass, Posidonia australis, in Cockburn Sound, Western Australia. Within the two sampled meadows, genetic diversity was moderate among the maternal shoots (R = 0·45 and 0·64) and extremely high in the embryos (R = 0·93-0·97). Both meadows exhibited a highly clumping (or phalanx) structure among clones, with spatial autocorrelation analysis showing significant genetic structure among shoots and embryos up to 10-15 m. Outcrossing rates were not significantly different from one. Pollen dispersal distances inferred from paternity assignment averaged 30·8 and 26·8 m, which was larger than the mean clone size (12·8 and 13·8 m). These results suggest highly effective movement of pollen in the water column. Despite strong clonal structure and moderate genetic diversity within meadows, hydrophilous pollination is an effective vector for completely outcrossed offspring. The different localized water conditions at each site (highly exposed conditions vs. weak directional flow) appear to have little influence on the success and pattern of successful pollination in the two meadows. © The Author 2014. Published by Oxford University Press on behalf of the Annals of Botany Company. All

  12. Stable isotopes indicate population structuring in the southwest Atlantic population of right whales (Eubalaena australis.

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    Morgana Vighi

    Full Text Available From the early 17th century to the 1970s southern right whales, Eubalaena australis, were subject to intense exploitation along the Atlantic coast of South America. Catches along this coast recorded by whalers originally formed a continuum from Brazil to Tierra del Fuego. Nevertheless, the recovery of the population has apparently occurred fragmentarily, and with two main areas of concentration, one off southern Brazil (Santa Catarina and another off central Argentina (Peninsula Valdés. This pattern suggests some level of heterogeneity amongst the population, which is apparently contradicted by records that traced individuals moving throughout the whole geographical extension covered by the species in the Southwest Atlantic. To test the hypothesis of the potential occurrence of discrete subpopulations exploiting specific habitats, we investigated N, C and O isotopic values in 125 bone samples obtained from whaling factories operating in the early 1970s in southern Brazil (n=72 and from contemporary and more recent strandings occurring in central Argentina (n=53. Results indicated significant differences between the two sampling areas, being δ13C and δ18O values significantly higher in samples from southern Brazil than in those from central Argentina. This variation was consistent with isotopic baselines from the two areas, indicating the occurrence of some level of structure in the Southwest Atlantic right whale population and equally that whales more likely feed in areas commonly thought to exclusively serve as nursing grounds. Results aim at reconsidering of the units currently used in the management of the southern right whale in the Southwest Atlantic Ocean. In the context of the current die-off affecting the species in Peninsula Valdés, these results also highlight the necessity to better understand movements of individuals and precisely identify their feeding areas.

  13. Produção de mudas de alface com biofertilizantes e substratos Production of lettuce plantlets using biofertilizer and substrates

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    Damiana Cleuma de Medeiros

    2007-09-01

    Full Text Available Com o objetivo de avaliar o desenvolvimento de mudas de alface que foram desenvolvidas em diferentes substratos e sob efeito de adubação foliar usando biofertilizantes, foi realizado o experimento em casa de vegetação da UFERSA. Utilizou-se o delineamento de blocos ao acaso com 4 repetições. Os tratamentos foram resultados de um fatorial 4 x 5 envolvendo tipos de substrato (composto orgânico, Gold Mix 11, areia lavada e húmus + areia lavada 1:1 e fertilizante foliar (organo-mineral comercial Fertamin, testemunha e três tipos de biofertilizantes. Foram feitas adubações foliares nas mudas, aos oito e aos 15 dias após a semeadura. O fertilizante organo-mineral comercial Fertamin apresentou os melhores resultados para massa seca da parte aérea, massa seca da raiz e número de folhas. Dentre os biofertilizantes, aquele à base de esterco de curral fresco, cinzas, leite e caldo de cana apresentou os menores valores para essas mesmas características avaliadas. O composto orgânico foi o que apresentou os maiores valores para todas as características avaliadas, exceto para comprimento da raiz.The development of lettuce plantlets was evaluated, employing different substrate types and applying foliar biofertilization. The research was done in a green house in Mossoró, Rio Grande do Norte State, Brazil. The experimental design was of randomized blocks with four replications. The treatments were composed of the interaction substract type (composed organic, Golden Mix 11, washed sand and humus + washed sand 1:1 and fertilizer (the commercial organo-mineral Fertamin, the witness and tree biofertilizer types. Plantlets were foliar fertilized 8 and 15 days after sowing date. Plantlets treated with Fertamim presented the best results in the dry matter of the aerial part, dry matter of roots and number of leaves. Among the biofertilizers, the one based on cattle manure, leached ash, milk and broth of sugar cane presented the lesser values for the

  14. Physiological responses in the banana plantlets treateds with strobilurinsRespostas fisiológicas em mudas de banananeira tratadas com estrobilurinas

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    Sivia Helena Modenese-Gorla da Silva

    2012-04-01

    Full Text Available There are reports that strobilurin besides having a fungicide effect can promote physiologic benefits to the plants. However, this effect on banana plants was not studied yet. The objective of the present study was to evaluate the effect of strobirulins on the physiology of banana plantlets. For this purpose, cultivar Grand Naine banana plantlets were transferred to pots containing substrate and kept in a nursery with 50% shading. The experimental design was a completely randomized design with three treatments (water, azoxystrobin and pyraclostrobin and five replications. The treatments were applied at 15, 30, 45, 60 and 75 days after transplanting at a dose 100 g a. i. ha-1 with manual spray. Plant height, pseudostem diameter, shoot dry matter in strobilurin treated plants were higher than the untreated plants, however, the effect of fungicide treatment was different, being the most pronounced effect of pyraclostrobin compared to azoxystrobin. Plants treated with pyraclostrobin had higher leaf area, nitrate reductase activity and chlorophyll content of leaf total nitrogen than the plants treated with azoxystrobin and water, which did not differ. Strobilurins affect the physiology of the banana plantlets differently, the effect being more pronounced by pyraclostrobin.Há relatos de que estrobilurinas, além de atuarem como fungicida, promovem benefícios fisiológicos às plantas. Assim sendo, o presente trabalho teve como objetivo avaliar o efeito de estrobirulinas na fisiologia de mudas de bananeira. Para tal, mudas micropropagadas da cultivar Grand Naine foram repicadas para vasos contendo substrato e mantidas em viveiro a 50% de sombreamento. O delineamento experimental foi inteiramente casualizado, com três tratamentos (água, azoxistrobina e piraclostrobina e cinco repetições. As estrobirulinas foram aplicadas com pulverizador manual aos 15, 30, 45, 60 e 75 dias após a repicagem, na dose de 100 g i. a. ha-1. A altura da planta, di

  15. Parasite body volume and infracommunity patterns in the southern pomfret Brama australis (Pisces: Bramidae Volumen corporal del parásito y patrones infracomunitarios en la reineta Brama australis (Pisces: Bramidae

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    MARIO GEORGE-NASCIMENTO

    2002-12-01

    Full Text Available The patterns of infracommunity descriptors (diversity, dominance, abundance are compared when calculated with the number and body volume of the parasites in a sample of 26 southern pomfrets Brama australis collected in the area off Talcahuano, Chile. No numerical infracommunity descriptor was correlated with its corresponding volumetric descriptor. This single result casts doubts about the general validity of the infracommunity patterns described in the literature so far, because they are almost exclusively based on the number of parasite individuals per individual host, restricting communication with ecologists that use density or other measures of ecological abundanceLos patrones de los descriptores infracomunitarios (diversidad, dominancia, abundancia son comparados cuando son calculados en base a los números o al volumen corporal de los parásitos en una muestra de 26 reinetas Brama australis recolectadas en el área de Talcahuano, Chile. Ningún descriptor infracomunitario calculado con el número de parásitos estuvo correlacionado con los basados en el volumen corporal de los parásitos. Este resultado siembra dudas acerca de la validez general que pueden tener los patrones infracomunitarios registrados en la literatura ya que están casi exclusivamente calculados con el número de parásitos por individuo hospedador, lo que dificulta aún más la comunicación con los ecólogos, que usan la densidad u otras medidas de abundancia ecológica

  16. The parasitic plant Cuscuta australis is highly insensitive to abscisic acid-induced suppression of hypocotyl elongation and seed germination.

    Science.gov (United States)

    Li, Juan; Hettenhausen, Christian; Sun, Guiling; Zhuang, Huifu; Li, Jian-Hong; Wu, Jianqiang

    2015-01-01

    Around 1% of angiosperms are parasitic plants. Their growth and development solely or partly depend on host plants from which they extract water, nutrients, and other molecules using a parasitic plant-specific organ, the haustorium. Strong depletion of nutrients can result in serious growth retardation and in some cases, death of the hosts. The genus Cuscuta (dodder) comprises about 200 holoparasitic species occurring on all continents. Their seedlings have no roots and cotyledons but are only string-like hypocotyls. When they contact suitable host plants, haustoria are formed and thereafter seedlings rapidly develop into vigorously growing branches without roots and leaves. This highly specialized lifestyle suggests that Cuscuta plants likely have unique physiology in development and stress responses. Using germination and seedling growth assays, we show that C. australis seeds and seedlings are highly insensitive to abscisic acid (ABA). Transcriptome analysis and protein sequence alignment with Arabidopsis, tomato, and rice homologs revealed that C. australis most likely consists of only four functional ABA receptors. Given that Cuscuta plants are no longer severely challenged by drought stress, we hypothesize that the ABA-mediated drought resistance pathway in Cuscuta spp. might have had degenerated over time during evolution.

  17. The parasitic plant Cuscuta australis is highly insensitive to abscisic acid-induced suppression of hypocotyl elongation and seed germination.

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    Juan Li

    Full Text Available Around 1% of angiosperms are parasitic plants. Their growth and development solely or partly depend on host plants from which they extract water, nutrients, and other molecules using a parasitic plant-specific organ, the haustorium. Strong depletion of nutrients can result in serious growth retardation and in some cases, death of the hosts. The genus Cuscuta (dodder comprises about 200 holoparasitic species occurring on all continents. Their seedlings have no roots and cotyledons but are only string-like hypocotyls. When they contact suitable host plants, haustoria are formed and thereafter seedlings rapidly develop into vigorously growing branches without roots and leaves. This highly specialized lifestyle suggests that Cuscuta plants likely have unique physiology in development and stress responses. Using germination and seedling growth assays, we show that C. australis seeds and seedlings are highly insensitive to abscisic acid (ABA. Transcriptome analysis and protein sequence alignment with Arabidopsis, tomato, and rice homologs revealed that C. australis most likely consists of only four functional ABA receptors. Given that Cuscuta plants are no longer severely challenged by drought stress, we hypothesize that the ABA-mediated drought resistance pathway in Cuscuta spp. might have had degenerated over time during evolution.

  18. Preliminary Study On Gonad Maturity Stages of the Sea Cucumber Paracaudina australis from Kenjeran Water, Surabaya, Indonesia

    Science.gov (United States)

    Widianingsih, Widianingsih; Zaenuri, Muhammad; Anggoro, Sutrisno; Pancasakti Kusumaningrum, Hermin; Hartati, Retno

    2018-02-01

    The holothurian Paracaudina australis is belong to family Caudinidae, ordo Molpadida and class Holothuroidea. This species is among the most common holothurian widely distributed in the tropical water. The purpose of this reseach is to do preliminary study on maturity stages of sea cucumber Paracaudina australis from Kenjeran Water, Surabaya, Indonesia. This research was conducted on April 2016. Samples were collected randomly on the Kenjeran Water, Surabaya. The result showed that there are five stages of gonad maturity. At the stage of maturity 1, the gonad was not clearly distinguished, there were unbranched small tubule. At the stage of maturity 2, there were small branched of tubules. At this stage, gonad can be differentiated between male and female. At the stage of maturity 3, tubule can been branched not only for male but also female. At the stage of maturity 4, the gonad was good mature, there were clearly branched tubule. At the stage of matury 5, there were generally had empty tubule except for a few relict unreleased spermatozoa. At female gonad, there were shrunken tubule and relict oocytes were presented in the lumen of the tubule.

  19. Bioactivity and chemical composition of the essential oil from the leaves of Guatteria australis A.St.-Hil.

    Science.gov (United States)

    Siqueira, Carlos Alberto Theodoro; Serain, Alessandra Freitas; Pascoal, Aislan Cristina Rheder Fagundes; Andreazza, Nathalia Luiza; de Lourenço, Caroline Caramano; Ruiz, Ana Lúcia T Góis; de Carvalho, João Ernesto; de Souza, Ana Cláudia Oliveira; Mesquita, Juliana Tonini; Tempone, Andre Gustavo; Salvador, Marcos José

    2015-01-01

    Essential oil from the leaves of Guatteria australis was obtained by hydrodistillation, analyzed by Gas Chromatography coupled to Mass Spectromery (GC-MS) and their antiproliferative, antileishmanial, antibacterial, antifungal and antioxidant activities were also evaluated. Twenty-three compounds were identified among which germacrene B (50.66%), germacrene D (22.22%) and (E)-caryophyllene (8.99%) were the main compounds. The highest antiproliferative activity was observed against NCI-ADR/RES (TGI = 31.08 μg/ml) and HT-29 (TGI = 32.81 μg/ml) cell lines. It also showed good antileishmanial activity against Leishmania infantum (IC50 = 30.71 μg/ml). On the other hand, the oil exhibited a small effect against Staphylococcus aureus ATCC 6538, S. aureus ATCC 14458 and Escherichia coli ATCC 10799 (MIC = 250 μg/ml), as well as small antioxidant activity (457 μmol TE/g) assessed through ORACFL assay. These results represent the first report regarding chemical composition and bioactivity of G. australis essential oil.

  20. Isolation of a Seawater Tolerant Leptospira spp. from a Southern Right Whale (Eubalaena australis).

    Science.gov (United States)

    Grune Loffler, Sylvia; Rago, Virginia; Martínez, Mara; Uhart, Marcela; Florin-Christensen, Monica; Romero, Graciela; Brihuega, Bibiana

    2015-01-01

    Leptospirosis is the most widespread zoonotic disease in the world. It is caused by pathogenic spirochetes of the genus Leptospira spp. and is maintained in nature through chronic renal infection of carrier animals. Rodents and other small mammals are the main reservoirs. Information on leptospirosis in marine mammals is scarce; however, cases of leptospirosis have been documented in pinniped populations from the Pacific coast of North America from southern California to British Columbia. We report the isolation of a Leptospira spp. strain, here named Manara, from a kidney sample obtained from a Southern Right Whale (Eubalaena australis) calf, which stranded dead in Playa Manara, Península Valdés, Argentina. This strain showed motility and morphology typical of the genus Leptospira spp. under dark-field microscopy; and grew in Ellinghausen-McCullough-Johnson-Harris (EMJH) medium and Fletcher medium after 90 days of incubation at 28°C. Considering the source of this bacterium, we tested its ability to grow in Fletcher medium diluted with seawater at different percentages (1%, 3%, 5%, 7% and 10% v/v). Bacterial growth was detected 48 h after inoculation of Fletcher medium supplemented with 5% sea water, demonstrating the halophilic nature of the strain Manara. Phylogenetic analysis of 16S rRNA gene sequences placed this novel strain within the radiation of the pathogenic species of the genus Leptospira spp., with sequence similarities within the range 97-100%, and closely related to L. interrogans. Two different PCR protocols targeting genus-specific pathogenic genes (G1-G2, B64I-B64II and LigB) gave positive results, which indicates that the strain Manara is likely pathogenic. Further studies are needed to confirm this possibility as well as determine its serogroup. These results could modify our understanding of the epidemiology of this zoonosis. Until now, the resistance and ability to grow in seawater for long periods of time had been proven for the strain

  1. Isolation of a Seawater Tolerant Leptospira spp. from a Southern Right Whale (Eubalaena australis.

    Directory of Open Access Journals (Sweden)

    Sylvia Grune Loffler

    Full Text Available Leptospirosis is the most widespread zoonotic disease in the world. It is caused by pathogenic spirochetes of the genus Leptospira spp. and is maintained in nature through chronic renal infection of carrier animals. Rodents and other small mammals are the main reservoirs. Information on leptospirosis in marine mammals is scarce; however, cases of leptospirosis have been documented in pinniped populations from the Pacific coast of North America from southern California to British Columbia. We report the isolation of a Leptospira spp. strain, here named Manara, from a kidney sample obtained from a Southern Right Whale (Eubalaena australis calf, which stranded dead in Playa Manara, Península Valdés, Argentina. This strain showed motility and morphology typical of the genus Leptospira spp. under dark-field microscopy; and grew in Ellinghausen-McCullough-Johnson-Harris (EMJH medium and Fletcher medium after 90 days of incubation at 28°C. Considering the source of this bacterium, we tested its ability to grow in Fletcher medium diluted with seawater at different percentages (1%, 3%, 5%, 7% and 10% v/v. Bacterial growth was detected 48 h after inoculation of Fletcher medium supplemented with 5% sea water, demonstrating the halophilic nature of the strain Manara. Phylogenetic analysis of 16S rRNA gene sequences placed this novel strain within the radiation of the pathogenic species of the genus Leptospira spp., with sequence similarities within the range 97-100%, and closely related to L. interrogans. Two different PCR protocols targeting genus-specific pathogenic genes (G1-G2, B64I-B64II and LigB gave positive results, which indicates that the strain Manara is likely pathogenic. Further studies are needed to confirm this possibility as well as determine its serogroup. These results could modify our understanding of the epidemiology of this zoonosis. Until now, the resistance and ability to grow in seawater for long periods of time had been proven

  2. The effect of kauri (Agathis australis) on grain size distribution and clay mineralogy of andesitic soils in the Waitakere Ranges, New Zealand

    NARCIS (Netherlands)

    Jongkind, A.G.; Buurman, P.

    2006-01-01

    Kauri (Agathis australis) is generally associated with intense podzolisation, but little research has been carried out to substantiate this. We studied soil profiles, grain size distribution patterns and clay mineralogy under kauri and broadleaf/tree fern vegetation in the Waitakere Ranges, North

  3. Aphid (Myzus persicae) feeding on the parasitic plant dodder (Cuscuta australis) activates defense responses in both the parasite and soybean host.

    Science.gov (United States)

    Zhuang, Huifu; Li, Juan; Song, Juan; Hettenhausen, Christian; Schuman, Meredith C; Sun, Guiling; Zhang, Cuiping; Li, Jing; Song, Dunlun; Wu, Jianqiang

    2018-06-01

    Dodders (Cuscuta spp.) are shoot holoparasites, whose haustoria penetrate host tissues to enable fusion between the parasite and host vascular systems, allowing Cuscuta to extract water, nutrients and other molecules from hosts. Aphids are piercing-sucking herbivores that use specialized stylets to feed on phloem sap. Aphids are known to feed on Cuscuta, but how Cuscuta and its host plant respond to aphids attacking the parasite was unknown. Phytohormone quantification, transcriptomic analysis and bioassays were performed to determine the responses of Cuscuta australis and its soybean (Glycine max) hosts to the feeding of green peach aphid (GPA; Myzus persicae) on C. australis. Decreased salicylic acid levels and 172 differentially expressed genes (DEGs) were found in GPA-attacked C. australis, and the soybean hosts exhibited increased jasmonic acid contents and 1015 DEGs, including > 100 transcription factor genes. Importantly, GPA feeding on C. australis increased the resistance of the soybean host to subsequent feeding by the leafworm Spodoptera litura and soybean aphid Aphis glycines, resulting in 21% decreased leafworm mass and 41% reduced aphid survival rate. These data strongly suggest that GPA feeding on Cuscuta induces a systemic signal, which is translocated to hosts and activates defense against herbivores. © 2018 The Authors. New Phytologist © 2018 New Phytologist Trust.

  4. Short-term and long-term effects of tannins on nitrogen mineralisation and litter decomposition in kauri (Agathis australis (D. Don) Lindl.) forests

    NARCIS (Netherlands)

    Verkaik, E.; Jongkind, A.G.; Berendse, F.

    2006-01-01

    Kauri (Agathis australis (D. Don) Lindl.) occurs naturally in the warm temperate forest of northern New Zealand where it grows mixed with angiosperm tree species. Below mature kauri trees thick organic layers develop in which large amounts of nitrogen are accumulated. This nitrogen seems to be

  5. Egg discrimination in the Australian reed warbler (Acrocephalus australis) : rejection response toward model and conspecific eggs depending on timing and mode of artificial parasitism

    NARCIS (Netherlands)

    Welbergen, J; Komdeur, J; Kats, R; Berg, M

    2001-01-01

    In a coevolutionary arms race between an interspecific brood parasite and its host species, bath are expected to evolve adaptations and counteradaptations. We studied egg discrimination in the Australian warbler (Acrocephalus australis). This species is currently not significantly parasitized by the

  6. The Spitzer survey of interstellar clouds in the Gould Belt. III. A multi-wavelength view of Corona Australis

    DEFF Research Database (Denmark)

    Peterson, Dawn E.; Caratti o Garatti, Alessio; Bourke, Tyler L.

    2011-01-01

    We present Spitzer Space Telescope IRAC and MIPS observations of a 0.85 deg2 field including the Corona Australis (CrA) star-forming region. At a distance of 130 pc, CrA is one of the closest regions known to be actively forming stars, particularly within its embedded association, the Coronet. Us...

  7. Debregeasia australis sp. nov. (Urticaceae), with a new synopsis of and a new key to the genus

    DEFF Research Database (Denmark)

    Wilmot-Dear, Christine Melanie; Friis, Ib

    2012-01-01

    A new species in the Old World genus Debregeasia (Urticaceae), D. australis Friis, Wilmot-Dear & C.J.Chen, based on material from forest habitats in eastern Queensland, Australia, is described, illustrated and mapped. A new synopsis of the genus and a new key to species recognised is provided as ...

  8. Temperature, energy acquisition and energy use in the Chilean silverside Basilichthys australis Eigenmann (Atherinopsidae Temperatura, adquisición de energía y uso de energía en el pejerrey chileno Basilichthys australis Eigenmann (Atherinopsidae

    Directory of Open Access Journals (Sweden)

    LEOPOLDO FUENTES

    2005-06-01

    Full Text Available We evaluated the influence of water temperature (Tw on the energy acquisition and use in the chilean silverside Basilichthys australis (Eigenmann 1927, an endemic species inhabiting freshwater ecosystems in Chile. We tested the effect of Tw (11.5, 14.0, 18.0, 22.0 and 26.0 °C on food intake, digestibility, food transit time and metabolic rate. As expected, this study demonstrated that many physiological variables under study were significantly affected by Tw, as well as the net energy balance of this species. Nevertheless, the net energy balance was not strictly related to the range of Tws evaluated. At Tws lower than 14 °C the energy budget was depressed, because food intake was lower than at Tws between 14 and 26 °C, where food intake was higher and independent of Tw. Consequently, at these temperatures the energy balance was positive and also independent of Tw. Physiologically, B. australis appears to be a tolerant species with respect to the wide range of water temperature in habitats at different depths. Thus, its distributions may extend through the entire profile of lakes and rivers, even in systems characterised by spatial and temporal thermal variabilityEn este trabajo evaluamos la influencia de la temperatura del agua (Ta en la adquisición de energía y su uso por parte del pejerrey chileno Basilichthys australis (Eigenmann 1927, una especie endémica que habita los ecosistemas dulceacuícolas de Chile. Investigamos el efecto de Ta (11,5, 14,0, 18,0, 22,0 y 26,0 °C en la ingesta de alimento, digestibilidad, tiempo de transito del alimento y tasa metabólica. De acuerdo a lo esperado, este estudio demostró que varias de las variables fisiológicas bajo estudio fueron significativamente afectadas por Ta, así como el balance energético de esta especie. Sin embargo, el balance neto de energía no estuvo estrictamente relacionado al rango de Tas evaluadas. En Tas inferiores a 14 °C el presupuesto de energía fue deprimido, debido a

  9. Echolocation in sympatric Peale's dolphins (Lagenorhynchus australis) and Commerson's dolphins (Cephalorhynchus commersonii) producing narrow-bandhigh-frequency clicks

    DEFF Research Database (Denmark)

    Kyhn, Line Anker; Jensen, Frants Havmand; Beedholm, Kristian

    2010-01-01

    -element hydrophone array from wild Peale's (Lagenorhynchus australis) and Commerson's (Cephalorhynchus commersonii) dolphins off the Falkland Islands. The centroid frequency was different between Commerson's (133±2kHz) and Peale's (129±3kHz) dolphins. The r.m.s. bandwidth was 12±3kHz for both species. The source...... level was higher for Peale's dolphin (185±6dB re 1 uPa p.-p.) than for Commerson's(177±5 dB re 1 uPa p.-p.). The mean directivity indexes were 25dB for both species. The relatively low source levels in combination with the high directivity index may be an adaptation to reduce clutter when foraging...

  10. Crassulacean acid metabolism enhances underwater photosynthesis and diminishes photorespiration in the aquatic plant Isoetes australis

    DEFF Research Database (Denmark)

    Pedersen, Ole; Rich, S.M.; Pulido Pérez, Cristina

    2011-01-01

    Underwater photosynthesis by aquatic plants is often limited by low availability of CO2, and photorespiration can be high. Some aquatic plants utilize crassulacean acid metabolism (CAM) photosynthesis. The benefits of CAM for increased underwater photosynthesis and suppression of photorespiration...... photorespiration was evident at a range of O2 concentrations, including values below air equilibrium. At a high O2 concentration of 2.2-fold the atmospheric equilibrium concentration, net photosynthesis was reduced substantially and, although it remained positive in leaves containing high malate concentrations...... were evaluated for Isoetes australis, a submerged plant that inhabits shallow temporary rock pools. • Leaves high or low in malate were evaluated for underwater net photosynthesis and apparent photorespiration at a range of CO2 and O2 concentrations. • CAM activity was indicated by 9.7-fold higher leaf...

  11. Carbon dioxide, temperature, salinity, and other variables collected via surface underway survey from Volunteer Observing Ship AURORA AUSTRALIS in the Southern Oceans (> 60 degrees South) from 1992-10-19 to 2001-12-12 (NODC Accession 0081031)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Surface and Atmospheric fCO2 measurements in the Southern Ocean during the VOS Project line onboard the oceanographic ship Aurora Australis.

  12. Моrрhоlоgy of inflorescensce and bluming peculiarities of Baptisia australis (L. R.Br. (Fabaceae introduced in М.М. Grishko National Botanical Garden NAS of Ukraine

    Directory of Open Access Journals (Sweden)

    Vira G. Mikolajchuk

    2014-04-01

    Full Text Available The structure of inflorescence in Baptisia australis introduced in conditions of M.M. Grishko National Botanical Garden in Kyiv has been studied. The dynamics of blooming and inflorescence development were analyzed.

  13. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the AURORA AUSTRALIS in the Indian Ocean from 1991-09-25 to 1991-10-27 (NODC Accession 0116370)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116370 includes chemical, discrete sample, physical and profile data collected from AURORA AUSTRALIS in the Indian Ocean from 1991-09-25 to...

  14. A new dolphin species, the Burrunan Dolphin Tursiops australis sp. nov., endemic to southern Australian coastal waters.

    Science.gov (United States)

    Charlton-Robb, Kate; Gershwin, Lisa-ann; Thompson, Ross; Austin, Jeremy; Owen, Kylie; McKechnie, Stephen

    2011-01-01

    Small coastal dolphins endemic to south-eastern Australia have variously been assigned to described species Tursiops truncatus, T. aduncus or T. maugeanus; however the specific affinities of these animals is controversial and have recently been questioned. Historically 'the southern Australian Tursiops' was identified as unique and was formally named Tursiops maugeanus but was later synonymised with T. truncatus. Morphologically, these coastal dolphins share some characters with both aforementioned recognised Tursiops species, but they also possess unique characters not found in either. Recent mtDNA and microsatellite genetic evidence indicates deep evolutionary divergence between this dolphin and the two currently recognised Tursiops species. However, in accordance with the recommendations of the Workshop on Cetacean Systematics, and the Unified Species Concept the use of molecular evidence alone is inadequate for describing new species. Here we describe the macro-morphological, colouration and cranial characters of these animals, assess the available and new genetic data, and conclude that multiple lines of evidence clearly indicate a new species of dolphin. We demonstrate that the syntype material of T. maugeanus comprises two different species, one of which is the historical 'southern form of Tursiops' most similar to T. truncatus, and the other is representative of the new species and requires formal classification. These dolphins are here described as Tursiops australis sp. nov., with the common name of 'Burrunan Dolphin' following Australian aboriginal narrative. The recognition of T. australis sp. nov. is particularly significant given the endemism of this new species to a small geographic region of southern and south-eastern Australia, where only two small resident populations in close proximity to a major urban and agricultural centre are known, giving them a high conservation value and making them susceptible to numerous anthropogenic threats.

  15. Caracterización estructural de un bosque de Podocarpus parlatorei y Juglans australis en Salta, Argentina

    Directory of Open Access Journals (Sweden)

    M.A. Pinazo

    2003-06-01

    Full Text Available Se estudió la estructura de bosques montanos ubicados en áreas con distinto grado de modificación humana en el Noroeste de Argentina (22º24’ S, 1550 msnm. Se utilizaron parcelas circulares para caracterizar los rodales. Los rodales fueron clasificados en función del área basal por especie. Se reconocieron cinco estructuras diferenciadas por la proporción de Juglans australis, Podocarpus parlatorei y de las especies tolerantes a la sombra Blepharocalyx salicifolius, Myrcianthes sp y Allophylus edulis. Se presentan datos de densidad y área basal de individuos con diámetro a la altura del pecho (DAP> 10 cm y la densidad de individuos con DAPStands of Subtropical Montane Cloud Forest were studied in areas under different land use regimes near Los Toldos (NW Argentina. Circular plots were used to calculate density and basal area of trees with dbh > 10 cm; and density of trees with dbh < 10 cm. The stands were classified and grouped as a function of basal area. Five structures were recognized, with different proportions of Juglans australis, Podocarpus parlatorei and shade-tolerant species like Blepharocalyx salicifolius, Allophylus edulis and Myrcianthes sp. Less disturbed area stands had a higher basal area and greater dbh for all species, and shade-tolerant species in the canopy. In the most disturbed area, all canopy species were shade intolerant and regeneration was dominated by shade-tolerant species. The differences in composition, basal area, stem diameter class distribution and regeneration indicate that the structure types corresponded to different stages of the successional process, and the regeneration of the most disturbed areas suggest a tendency towards the composition of mature forest.

  16. A new dolphin species, the Burrunan Dolphin Tursiops australis sp. nov., endemic to southern Australian coastal waters.

    Directory of Open Access Journals (Sweden)

    Kate Charlton-Robb

    Full Text Available Small coastal dolphins endemic to south-eastern Australia have variously been assigned to described species Tursiops truncatus, T. aduncus or T. maugeanus; however the specific affinities of these animals is controversial and have recently been questioned. Historically 'the southern Australian Tursiops' was identified as unique and was formally named Tursiops maugeanus but was later synonymised with T. truncatus. Morphologically, these coastal dolphins share some characters with both aforementioned recognised Tursiops species, but they also possess unique characters not found in either. Recent mtDNA and microsatellite genetic evidence indicates deep evolutionary divergence between this dolphin and the two currently recognised Tursiops species. However, in accordance with the recommendations of the Workshop on Cetacean Systematics, and the Unified Species Concept the use of molecular evidence alone is inadequate for describing new species. Here we describe the macro-morphological, colouration and cranial characters of these animals, assess the available and new genetic data, and conclude that multiple lines of evidence clearly indicate a new species of dolphin. We demonstrate that the syntype material of T. maugeanus comprises two different species, one of which is the historical 'southern form of Tursiops' most similar to T. truncatus, and the other is representative of the new species and requires formal classification. These dolphins are here described as Tursiops australis sp. nov., with the common name of 'Burrunan Dolphin' following Australian aboriginal narrative. The recognition of T. australis sp. nov. is particularly significant given the endemism of this new species to a small geographic region of southern and south-eastern Australia, where only two small resident populations in close proximity to a major urban and agricultural centre are known, giving them a high conservation value and making them susceptible to numerous anthropogenic

  17. Comparative Digital Gene Expression Analysis of Tissue-Cultured Plantlets of Highly Resistant and Susceptible Banana Cultivarsin Response to Fusarium oxysporum

    Directory of Open Access Journals (Sweden)

    Yuqing Niu

    2018-01-01

    Full Text Available Banana Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc is one of the most destructive soil-borne diseases. In this study, young tissue-cultured plantlets of banana (Musa spp. AAA cultivars differing in Foc susceptibility were used to reveal their differential responses to this pathogen using digital gene expression (DGE. Data were evaluated by various bioinformatic tools (Venn diagrams, gene ontology (GO annotation and Kyoto encyclopedia of genes and genomes (KEGG pathway analyses and immunofluorescence labelling method to support the identification of gene candidates determining the resistance of banana against Foc. Interestingly, we have identified MaWRKY50 as an important gene involved in both constitutive and induced resistance. We also identified new genes involved in the resistance of banana to Foc, including several other transcription factors (TFs, pathogenesis-related (PR genes and some genes related to the plant cell wall biosynthesis or degradation (e.g., pectinesterases, β-glucosidases, xyloglucan endotransglucosylase/hydrolase and endoglucanase. The resistant banana cultivar shows activation of PR-3 and PR-4 genes as well as formation of different constitutive cell barriers to restrict spreading of the pathogen. These data suggest new mechanisms of banana resistance to Foc.

  18. Interactive effects of aluminum and cadmium on phenolic compounds, antioxidant enzyme activity and oxidative stress in blueberry (Vaccinium corymbosum L.) plantlets cultivated in vitro.

    Science.gov (United States)

    Manquián-Cerda, K; Cruces, E; Escudey, M; Zúñiga, G; Calderón, R

    2018-04-15

    To evaluate the potential role of phenolic compounds in Al and Cd stress tolerance mechanisms, Vaccinium corymbosum cv. Legacy plantlets were exposed to different metal concentrations. The present study used an in vitro plant model to test the effects of the following treatments: 100μM Al; 100μMAl + 50μMCd; and 100μMAl + 100μMCd during periods of 7, 14, 21 and 30 days. The oxidative damage was determined by the accumulation of malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ). The antioxidant activity values were determined using 1,1-diphenyl-2-picrylhydrazine (DPPH) and the ferric reducing antioxidant power test (FRAP). Additionally, the phenolic compound concentrations were determined using HPLC-DAD. The exposure to Al and Cd increased the MDA and H 2 O 2 contents differentially, while the antioxidant capacity values showed differences between DPPH and FRAP with the largest changes in FRAP relative to Cd. SOD had the highest activity in the first 7 days, leading to a significant increase in phenolic compounds observed after 14 days, and chlorogenic acid was the major compound identified. Our results revealed that phenolic compounds seem to play an important role in the response to ROS. Therefore, the mechanisms of tolerance to Al and Cd in V. corymbosum will be determined by the type of metal and time of exposure. Copyright © 2017 Elsevier Inc. All rights reserved.

  19. Mass propagation of in vitro plantlets and extraction of bioactive compounds from Eurycoma Longifolia Jack (Tongkat Ali) using cell and organ cultures

    International Nuclear Information System (INIS)

    Sobri Hussein; Rusli Ibrahim

    2006-01-01

    Eurycoma longifolia Jack or better known in Malaysia as Tongkat Ali is an important herbal plant that owes its popularity locally and worldwide to its aphrodisiac claim and has been sought after as an essential component for the treatment of anti-malarial, anti-ulcer, anti-tumour promoting and anti-parasitic agent and also as health supplements. Quassinoids and canthin-6-one alkaloids contained in Tongkat Ali roots are the main substances having active functions for human body. Propagation is commonly done through seeds or some vegetative means such as cuttings of stems and root, grafting or layering. However, propagation by conventional method is inadequate to meet the demand due to low viability, low germination rate, delayed rooting and long life cycle. Tissue culture has been successfully applied for mass production of plantlets and extraction of many useful secondary metabolites, including pharmaceuticals, pigments and other fine chemicals. Tongkat Ali active substances have also been derived through cell culture technology but its processes need to be optimized before the product can be commercialized. Cell and organ cultures have been proven to be an efficient alternative method for biomass production due to its fast growth and stable metabolite production. In view of its potential commercial value as a plantation crop as well as to conserve its germplasm, micropropagation technique using cell culture system is an attractive method for regeneration and mass propagation. (Author)

  20. Effect of applied synthetic auxin on root growth in plantlet propagation by cuttage and tissue culture; Sashiki to soshiki baiyo ni okeru gosei auxin rui no shiyo koka

    Energy Technology Data Exchange (ETDEWEB)

    Shoji, K; Yoshihara, T [Central Research Institute of Electric Power Industry, Tokyo (Japan)

    1996-12-01

    The effect of synthetic plant hormone 4-C1-IAA and TFIBA on root growth in plantlet propagation was clarified by the cuttage and the issue culture of strawberry seedling production. A periwinkle, vine, and azalea are the effect of 4-C1-IAA on root growth, and a promotion effect was recognized for rooting and root elongation. The concentration of 4-C1-IAA in which the growth promotion effect of a root most appears varies depending on the species of a plant. The concentration of a periwinkle was 20 ppm, and that of an azalea was 2000 ppm. The growth promotion effect of a root in 4-C1-IAA and TFIBA was compared with IBA for an azalea. The result showed that 4-C1-IAA is the same in the effect as IBA and that TFIBA is higher than for IBA. The growth of a vine`s terminal bud was promoted by the effect of TFIBA on root growth, and the callus occurring when IBA was treated was not formed. The rooting of a strawberry was promoted by the effect of TFIBA on root growth. The combined use of TFIBA and BA promotes the growth of a side bud and forms a multi-bud plant. However, rooting was inhibited. The callus caused by the effect of BA on root growth could be suppressed through the combined use with TFIBA. 6 refs., 7 figs., 2 tabs.

  1. Structural Interaction Between GFP-Labeled Diazotrophic Endophytic Bacterium Herbaspirillum seropedicae RAM10 and Pineapple Plantlets ‘VitóRia’

    Science.gov (United States)

    Estrela Borges Baldotto, Lílian; Lopes Olivares, Fábio; Bressan-Smith, Ricardo

    2011-01-01

    The events involved in the structural interaction between the diazotrophic endophytic bacterium Herbaspirillum seropedicae, strain RAM10, labeled with green fluorescent protein, and pineapple plantlets ‘Vitória’ were evaluated by means of bright-field and fluorescence microscopy, combined with scanning electron microscopy for 28 days after inoculation. After 6 hours of inoculation, H. seropedicae was already adhered to the roots, colonizing mainly root hair surface and bases, followed by epidermal cell wall junctions. Bacteria adherence in the initial periods occurred mainly in the form of solitary cells and small aggregates with pleomorphic cells. Bacteria infection of root tissue occurred through the cavities caused by the disruption of epidermal cells during the emergence of lateral roots and the endophytic establishment by the colonization of intercellular spaces of the cortical parenchyma. Moreover, within 1 day after inoculation the bacteria were colonizing the shoots. In this region, the preferred sites of epiphytic colonization were epidermal cell wall junctions, peltate scutiform trichomes and non-glandular trichomes. Subsequently, the bacteria occupied the outer periclinal walls of epidermal cells and stomata. The penetration into the shoot occurred passively through stoma aperture followed by the endophytic establishment on the substomatal chambers and spread to the intercellular spaces of spongy chlorenchyma. After 21 days of inoculation, bacterial biofilm were seen at the root hair base and on epidermal cell wall surface of root and leaf, also confirming the epiphytic nature of H. seropedicae. PMID:24031612

  2. Role of solid-phase microextraction in the identification of highly volatile pheromones of two Rhinoceros beetles Scapanes australis and Strategus aloeus (Coleoptera, Scarabaeidae, Dynastinae).

    Science.gov (United States)

    Rochat, D; Ramirez-Lucas, P; Malosse, C; Aldana, R; Kakul, T; Morin, J P

    2000-07-14

    Solid-phase microextraction (SPME) samplings from live insects or natural secretion allowed one to identify the aggregation pheromones of the pest beetles Scapanes australis and Strategus aloeus by efficient and rapid isolation of their highly volatile (72 < M(r) < 116) components. S. australis male pheromone was identified as a 84:12:4 (w/w) mixture of 2-butanol [67:33 (R)-(-):(S)-(+) ratio], 3-hydroxy-2-butanone and 2,3-butanediol [43:17:40 (R,R)-(-):(S,S)-(+):meso ratio], and S. aloeus pheromone as a 95.5:4.0:0.5 (w/w) mixture of 2-butanone, 3-pentanone and sec.-butyl acetate by GC-MS using conventional and chiral capillary columns. This is the first report of Scarabaeidae pheromones based on such small and common molecules.

  3. Effects of Organic and Inorganic Nitrogen on the Growth and Production of Domoic Acid by Pseudo-nitzschia multiseries and P. australis (Bacillariophyceae) in Culture.

    Science.gov (United States)

    Martin-Jézéquel, Véronique; Calu, Guillaume; Candela, Leo; Amzil, Zouher; Jauffrais, Thierry; Séchet, Véronique; Weigel, Pierre

    2015-11-26

    Over the last century, human activities have altered the global nitrogen cycle, and anthropogenic inputs of both inorganic and organic nitrogen species have increased around the world, causing significant changes to the functioning of aquatic ecosystems. The increasing frequency of Pseudo-nitzschia spp. in estuarine and coastal waters reinforces the need to understand better the environmental control of its growth and domoic acid (DA) production. Here, we document Pseudo-nitzschia spp. growth and toxicity on a large set of inorganic and organic nitrogen (nitrate, ammonium, urea, glutamate, glutamine, arginine and taurine). Our study focused on two species isolated from European coastal waters: P. multiseries CCL70 and P. australis PNC1. The nitrogen sources induced broad differences between the two species with respect to growth rate, biomass and cellular DA, but no specific variation could be attributed to any of the inorganic or organic nitrogen substrates. Enrichment with ammonium resulted in an enhanced growth rate and cell yield, whereas glutamate did not support the growth of P. multiseries. Arginine, glutamine and taurine enabled good growth of P. australis, but without toxin production. The highest DA content was produced when P. multiseries grew with urea and P. australis grew with glutamate. For both species, growth rate was not correlated with DA content but more toxin was produced when the nitrogen source could not sustain a high biomass. A significant negative correlation was found between cell biomass and DA content in P. australis. This study shows that Pseudo-nitzschia can readily utilize organic nitrogen in the form of amino acids, and confirms that both inorganic and organic nitrogen affect growth and DA production. Our results contribute to our understanding of the ecophysiology of Pseudo-nitzschia spp. and may help to predict toxic events in the natural environment.

  4. Temperature, salinity and other variables collected from underway - surface observations using PAR Sensor and other instruments from the AURORA AUSTRALIS, NOAA Ship DISCOVERER and others in the Bering Sea, Caribbean Sea and others from 1994-01-28 to 2004-07-02 (NODC Accession 0109923)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0109923 includes biological, chemical, meteorological, physical and underway - surface data collected from AURORA AUSTRALIS, NOAA Ship DISCOVERER,...

  5. Assessment of Genetic Fidelity in Rauvolfia serpentina Plantlets Grown from Synthetic (Encapsulated Seeds Following in Vitro Storage at 4 °C

    Directory of Open Access Journals (Sweden)

    Mohammad Anis

    2012-05-01

    Full Text Available An efficient method was developed for plant regeneration and establishment from alginate encapsulated synthetic seeds of Rauvolfia serpentina. Synthetic seeds were produced using in vitro proliferated microshoots upon complexation of 3% sodium alginate prepared in Llyod and McCown woody plant medium (WPM and 100 mM calcium chloride. Re-growth ability of encapsulated nodal segments was evaluated after storage at 4 °C for 0, 1, 2, 4, 6 and 8 weeks and compared with non-encapsulated buds. Effects of different media viz; Murashige and Skoog medium; Lloyd and McCown woody Plant medium, Gamborg’s B5 medium and Schenk and Hildebrandt medium was also investigated for conversion into plantlets. The maximum frequency of conversion into plantlets from encapsulated nodal segments stored at 4 °C for 4 weeks was achieved on woody plant medium supplement with 5.0 μM BA and 1.0 μM NAA. Rooting in plantlets was achieved in half-strength Murashige and Skoog liquid medium containing 0.5 μM indole-3-acetic acid (IAA on filter paper bridges. Plantlets obtained from stored synseeds were hardened, established successfully ex vitro and were morphologically similar to each other as well as their mother plant. The genetic fidelity of Rauvolfia clones raised from synthetic seeds following four weeks of storage at 4 °C were assessed by using random amplified polymorphic DNA (RAPD and inter-simple sequence repeat (ISSR markers. All the RAPD and ISSR profiles from generated plantlets were monomorphic and comparable to the mother plant, which confirms the genetic stability among the clones. This synseed protocol could be useful for establishing a particular system for conservation, short-term storage and production of genetically identical and stable plants before it is released for commercial purposes.

  6. Bioinformatic prediction of G protein-coupled receptor encoding sequences from the transcriptome of the foreleg, including the Haller's organ, of the cattle tick, Rhipicephalus australis.

    Directory of Open Access Journals (Sweden)

    Sergio Munoz

    Full Text Available The cattle tick of Australia, Rhipicephalus australis, is a vector for microbial parasites that cause serious bovine diseases. The Haller's organ, located in the tick's forelegs, is crucial for host detection and mating. To facilitate the development of new technologies for better control of this agricultural pest, we aimed to sequence and annotate the transcriptome of the R. australis forelegs and associated tissues, including the Haller's organ. As G protein-coupled receptors (GPCRs are an important family of eukaryotic proteins studied as pharmaceutical targets in humans, we prioritized the identification and classification of the GPCRs expressed in the foreleg tissues. The two forelegs from adult R. australis were excised, RNA extracted, and pyrosequenced with 454 technology. Reads were assembled into unigenes and annotated by sequence similarity. Python scripts were written to find open reading frames (ORFs from each unigene. These ORFs were analyzed by different GPCR prediction approaches based on sequence alignments, support vector machines, hidden Markov models, and principal component analysis. GPCRs consistently predicted by multiple methods were further studied by phylogenetic analysis and 3D homology modeling. From 4,782 assembled unigenes, 40,907 possible ORFs were predicted. Using Blastp, Pfam, GPCRpred, TMHMM, and PCA-GPCR, a basic set of 46 GPCR candidates were compiled and a phylogenetic tree was constructed. With further screening of tertiary structures predicted by RaptorX, 6 likely GPCRs emerged and the strongest candidate was classified by PCA-GPCR to be a GABAB receptor.

  7. Genetic variation within three populations of Phycella australis (Phil. Ravenna from Biobío Region, Chile, evaluated using ISSR markers

    Directory of Open Access Journals (Sweden)

    Cristian Flores

    2013-03-01

    Full Text Available Phycella australis (Phil. Ravenna is a Chilean plant with high ornamental potential; however, the intensive extraction as a cut flower might be detrimental for the conservational state by ignoring the state of genetic variation. The objective of this investigation was to assess genetic variability within and between three populations of P. australis in the Biobío Region using inter-simple sequence repeat (ISSR markers. The evaluated areas correspond to three locations in the province of Concepción, Biobío Region: Desembocadura (36°48' S, 73°10' W, Santa Juana (36°58' S, 72°58' W, and Lipinhue (37°00' S, 72°58' W. Six ISSR primers were used obtaining 51 fragments, from which 72.5% were polymorphic. From the three evaluated sites Santa Juana showed a higher percentage of polymorphic loci (76.47%. From this variability, 83% belong to within population variability and only 17% belong to variability between populations. The dendrogram generated using the unweighted pair group method with arithmetic mean (UPGMA method, grouped Lipinhue and Santa Juana sites together, which agrees with the geographic locations. This investigation proved that P. australis has high genetic variability despite the exploitation for economic purposes.

  8. The effects of short term exposure to Bass Strait crude oil and Corexit 8667 on benthic community metabolism in Posidonia australis Hook. f. dominated microcosms

    Energy Technology Data Exchange (ETDEWEB)

    Hatcher, A.I.; Larkum, A.W.D.

    1982-03-01

    Microcosms consisting of a sublittoral seagrass meadow from Botany Bay, N.S.W. were maintained in aquaria in a controlled environment room from March to August 1979. Oxygen production and consumption in the microcosms and leaf turnover of the seagrass, Posidonia australis Hook.f. were measured before, during and after a 7-day treatment in June with Bass Strait crude oil and the dispersant Corexit 8667. Four microcosms received oil and two of these received dispersant. The leaf turnover of P. australis was not significantly affected by the addition of oil and dispersant. Photosynthetic oxygen production decreased and respiration increased in the microcosms during treatment. In August, 40 days after treatment, oxygen production rates and P/R ratios in the oil-treated microcosms were higher than rates measured before treatment. The oil-and-dispersant-treated microcosms did not show this trend. The results of this study indicate that a more severe stress is placed on the P. australis dominated benthic community by oil and dispersant than by oil alone.

  9. Organic geochemistry of resins from modern Agathis australis and Eocene resins from New Zealand: Diagenetic and taxonomic implications

    Science.gov (United States)

    Lyons, P.C.; Mastalerz, Maria; Orem, W.H.

    2009-01-01

    A maturation series of resins and fossil resins from New Zealand, ranging in age from Modern to Eocene and ranging from uncoalified to high volatile C bituminous coal, were analyzed by elemental, pyrolysis-gas chromatography (Py-GC), Fourier Transform infrared (FTir), and solid-state 13C nuclear magnetic resonance (13C NMR) techniques. For comparison, four resin samples from the Latrobe Valley, Australia, were analyzed. All of the resins and fossil resins of this study show very high H/C atomic ratios, and are characterized by dominant peaks in the 10-60??ppm range of solid-state 13C NMR spectra and prominent bands in the aliphatic stretching region (2800-3000??cm- 1) of FTir spectra, all indicating a highly aliphatic molecular structure. The 13C NMR and FTir data indicate a diterpenoid structure for these resins. There is an abrupt loss of oxygen that occurs at the Lignite A/Subbituminous C stage, which is attributed to a dramatic loss of carboxyl (COOH) from the diterpenoid molecule. This is a new finding in the diagenesis of resins. This important loss in oxygenated functional groups is attributed to a maturation change. Also, there is a progressive loss of exomethylene (CH2) groups with increasing degree of maturation, as shown by both 13C NMR and FTir data. This change has been noted by previous investigators. Exomethylene is absent in the fossil resins from the Eocene high volatile C bituminous coals. This progressive loss is characteristic of Class I resinites. FTir data indicate that the oxygenated functional groups are strong in all the resin samples except the fossil resin from high volatile C bituminous coal. This important change in oxygenated functional groups is attributed to maturation changes. The 13C NMR and FTir data indicate there are minor changes in the Agathis australis resin from the living tree and soil, which suggests that alteration of A. australis resins begins shortly after deposition in the soil for as little as 1000??years. The Morwell

  10. Organic geochemistry of resins from modern Agathis australis and Eocene resins from New Zealand: Diagenetic and taxonomic implications

    Energy Technology Data Exchange (ETDEWEB)

    Lyons, Paul C. [Lyons and Associates Consultants, 206 Amber Road, Middleboro, MA 02346 (United States); Mastalerz, Maria [Indiana Geological Survey, Indiana University, 611 North Walnut Grove, Bloomington, IN 47405 (United States); Orem, William H. [U.S. Geological Survey, MS 956 National Center, Reston, VA 20192 (United States)

    2009-10-01

    A maturation series of resins and fossil resins from New Zealand, ranging in age from Modern to Eocene and ranging from uncoalified to high volatile C bituminous coal, were analyzed by elemental, pyrolysis-gas chromatography (Py-GC), Fourier Transform infrared (FTir), and solid-state {sup 13}C nuclear magnetic resonance ({sup 13}C NMR) techniques. For comparison, four resin samples from the Latrobe Valley, Australia, were analyzed. All of the resins and fossil resins of this study show very high H/C atomic ratios, and are characterized by dominant peaks in the 10-60 ppm range of solid-state {sup 13}C NMR spectra and prominent bands in the aliphatic stretching region (2800-3000 cm{sup -} {sup 1}) of FTir spectra, all indicating a highly aliphatic molecular structure. The {sup 13}C NMR and FTir data indicate a diterpenoid structure for these resins. There is an abrupt loss of oxygen that occurs at the Lignite A/Subbituminous C stage, which is attributed to a dramatic loss of carboxyl (COOH) from the diterpenoid molecule. This is a new finding in the diagenesis of resins. This important loss in oxygenated functional groups is attributed to a maturation change. Also, there is a progressive loss of exomethylene (CH{sub 2}) groups with increasing degree of maturation, as shown by both {sup 13}C NMR and FTir data. This change has been noted by previous investigators. Exomethylene is absent in the fossil resins from the Eocene high volatile C bituminous coals. This progressive loss is characteristic of Class I resinites. FTir data indicate that the oxygenated functional groups are strong in all the resin samples except the fossil resin from high volatile C bituminous coal. This important change in oxygenated functional groups is attributed to maturation changes. The {sup 13}C NMR and FTir data indicate there are minor changes in the Agathis australis resin from the living tree and soil, which suggests that alteration of A. australis resins begins shortly after deposition

  11. Comparative morphophysiological evaluation of the testis of adult Wistar rats fed low protein-energy diet and dosed with aqueous extracts of Cuscuta australis.

    Science.gov (United States)

    Ozegbe, P C; Omirinde, J O

    2012-12-18

    Cuscuta australis (C. australis) seed and stem are historically used by the local population as dietary supplement for the management of infertility. This study, therefore, evaluated the effect of orally administered aqueous extracts of C. australis seed and stem, 300 mg/kg body weight/day for seven days, on the testis of the adult Wistar rat fed either low or normal protein-energy diets. The control group received water. The relative weight of the testis was non-significantly increased (p>0.05) in the Low Protein-energy diet-Water-treated (LPWA), Low Protein-energy diet-Seed-treated (LPSE) and Normal Protein-energy diet-Seed-treated (NPSE) groups relative to the Normal Protein-energy diet-Water-treated (NPWA). The weight of the testis was also non-significantly increased (p˃0.05) in the Low Protein-energy diet-Stem-treated (LPST), but decreased in the Normal Protein-energy diet-Stem-treated (NPST), relative to LPWA and NPWA. Heights of germinal epithelium were significantly decreased (p<0.05) in the LPWA, LPSE and LPST relative to the NPWA, NPSE and NPST. Diet significantly influenced (p<0.001) the effect of stem extract on the height of germinal epithelium. The NPSE, LPSE, NPST, LPST and LPWA showed significantly decreased (p<0.001) plasma levels of luteinizing hormone (LH) and follicle stimulating hormone (FSH) relative to NPWA. The LPWA, LPSE and NPST also showed significantly decreased (p<0.001) levels of testosterone relative to NPWA and LPST. Diet significantly influenced (p<0.001) the effect of seed on the level of LH. Seed-diet interactions significantly affected the levels of FSH (p<0.001) and LH (p<0.05), but not testosterone. Diet significantly influenced (p<0.001) the effects of stem extract on the levels of FSH, LH and testosterone. Stem-diet interactions significantly affected (p<0.001) the levels of FSH, LH and testosterone. Our data suggest that the aqueous extract of C. australis stem is more potent than the seed extract and that dietary protein

  12. Livestock as a potential biological control agent for an invasive wetland plant

    NARCIS (Netherlands)

    Silliman, Brian R.; Mozdzer, Thomas; Angelini, Christine; Brundage, Jennifer E.; Esselink, Peter; Bakker, Jan P.; Gedan, Keryn B.; van de Koppel, Johan; Baldwin, Andrew H.

    2014-01-01

    Invasive species threaten biodiversity and incur costs exceeding billions of US$. Eradication efforts, however, are nearly always unsuccessful. Throughoutmuch of North America, land managers have used expensive, and ultimately ineffective, techniques to combat invasive Phragmites australis in

  13. Biogenic silica in tidal freshwater marsh sediments and vegetation (Schelde estuary, Belgium)

    NARCIS (Netherlands)

    Struyf, E.; van Damme, S.; Gribsholt, B.; Middelburg, J.J.; Meire, P.

    2005-01-01

    To date, estuarine ecosystem research has mostly neglected silica cycling in freshwater intertidal marshes. However, tidal marshes can store large amounts of biogenic silica (BSi) in vegetation and sediment. BSi content of the typical freshwater marsh plants Phragmites australis, Impatiens

  14. REMOTE DETENTION OF INVASIVE AND OPPORTUNISTIC PLANT SPECIES IN GREAT LAKES COASTAL WETLANDS

    Science.gov (United States)

    Invasive and opportunistic plant species have been associated with wetland disturbance. Increases in the abundance of plant species such as common reed (Phragmites australis) in coastal Great Lakes wetlands are hypothesized to occur with shifts toward drier hydrologic regimes, fr...

  15. Livestock as a potential biological control agent for an invasive wetland plant

    NARCIS (Netherlands)

    Silliman, B.R.; Mozdzer, T.; Angelini, C.; Brundage, J.E.; Esselink, P.; Bakker, J.P.; Gedan, K.B.; van de Koppel, J.; Baldwin, A.H.

    2014-01-01

    Invasive species threaten biodiversity and incur costs exceeding billions of US$. Eradication efforts, however, are nearly always unsuccessful. Throughout much of North America, land managers have used expensive, and ultimately ineffective, techniques to combat invasive Phragmites australis in

  16. Red fox ( Vulpes vulpes L.) favour seed dispersal, germination and seedling survival of Mediterranean Hackberry ( Celtis australis L.)

    Science.gov (United States)

    Juan, Traba; Sagrario, Arrieta; Jesús, Herranz; Cristina, Clamagirand M.

    2006-07-01

    Seeds of the Mediterranean Hackberry Celtis australis are often encountered in fox faeces. In order to evaluate the effect of gut transit on the size of seeds selected, the rates and speed of germination and on the survival of the seedlings, Mediterranean Hackberry seeds from fox faeces were germinated in a greenhouse. The results were compared with those of seeds taken from ripe, uneaten fruits. Fox-dispersed seeds were smaller and lighter than the control ones and had higher (74% vs. 57%) and more rapid germination (74.5 days vs. 99.2 days). Seedlings from fox-dispersed seeds showed significantly greater survival by the end of the study period (74.1% vs. 43.6%) than the control ones. Survival in seedlings from fox-dispersed seeds was related to germination date, late seedlings showing poorer survival. This relationship was not observed away in the control seedlings. Seed mass did not affect seedling survival. Seedling arising from fox-dispersed seeds grew faster than control ones. These results suggest that fox can play a relevant role as seed disperser of Mediterranean Hackberry.

  17. Kelp and dolphin gulls cause perineal wounds in South American fur seal pups (Arctocephalus australis) at Guafo Island, Chilean Patagonia.

    Science.gov (United States)

    Seguel, Mauricio; Muñoz, Francisco; Montalva, Felipe; Perez-Venegas, Diego; Pavés, Héctor; Gottdenker, Nicole

    2017-07-01

    During five reproductive seasons, we documented the presence, extent and origin of perineal wounds in South American fur seal pups ( Arctocephalus australis ) on Guafo Island, Northern Chilean Patagonia. The seasonal prevalence of perineal wounds ranged from 5 to 9%, and new cases were more common at the end of the breeding season (February), when pups were on average two months old and were actively expelling hookworms ( Uncinaria sp). Histologically, wounds corresponded to marked ulcerative lymphoplasmacytic and histiocytic dermatitis with granulation tissue and mixed bacterial colonies. In 2015 and 2017, kelp gulls ( Larus dominicanus ) and dolphin gulls ( Leucophaeus scoresbii ) were observed picking and wounding the perineal area of marked pups. This behaviour occurred more frequently after the pups' defecation, when sea gulls engaged in consumption of pups' faeces. The affected pups usually had moderate to marked hookworm infections along with bloody diarrhoea and anaemia. Pups with severe wounds (23% of affected animals) had swollen perineal areas and signs of secondary systemic bacterial infection. We propose that seagulls on Guafo Island have learned to consume remains of blood and parasites in the faeces of pups affected by hookworm infection, causing perineal wounds during this process. We conclude that this perineal wounding is an unintentional, occasional negative effect of an otherwise commensal gull-fur seal relationship.

  18. Coastal upwelling linked to toxic Pseudo-nitzschia australis blooms in Los Angeles coastal waters, 2005-2007

    KAUST Repository

    Schnetzer, Astrid; Jones, Burton; Schaffner, Rebecca A.; Cetinić, Ivona; Fitzpatrick, Elizabeth; Miller, Peter E.; Seubert, Erica L.; Caron, David A.

    2013-01-01

    Harmful algal blooms dominated by the diatom Pseudo-nitzschia spp. have become a perennial but variable event within surface waters near the greater Los Angeles area. Toxic blooms during spring seasons from 2005 to 2007 varied strongly in their overall toxicity and duration. Differences in bloom dynamics were linked to differences in storm-induced river discharge following episodic rain events and coastal upwelling, both major coastal processes that led to the injection of nutrients into coastal surface waters. Heavy river runoff during early 2005, a record-rainfall year, favored a phytoplankton community mainly comprised of algal taxa other than Pseudo-nitzschia. The spring bloom during 2005 was associated with low domoic acid surface concentrations and minor contributions of (mainly) P. delicatissima to the diatom assemblage. In contrast, highly toxic P. australis-dominated blooms during spring seasons of 2006 and 2007 were linked to strong upwelling events. River discharge quotas in 2006 and 2007, in contrast to 2005, fell well below annual averages for the region. Surface toxin levels were linked to colder, more saline (i.e. upwelled) water over the 3-year study, but no such consistent relationship between domoic acid levels and other physiochemical parameters, such as macronutrient concentrations or nutrient ratios, was observed. © The Author 2013. Published by Oxford University Press. All rights reserved.

  19. Registros de enredamentos de baleias-franca, Eubalaena australis (CETACEA, MISTICETI, na temporada reprodutiva de 2010, em Santa Catarina, Brasil

    Directory of Open Access Journals (Sweden)

    Mônica Pontalti

    2011-03-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2011v24n2p109 Baleias-francas (Eubalaena australis sofrem diferentes pressões antrópicas como poluição, tráfego de embarcações e emalhe em redes de pesca. Casos de enredamentos em baleias-francas são crescentes no litoral sul de Santa Catarina, podendo levar a encalhes e morte. Durante a temporada reprodutiva de 2010, foram registrados seis casos de emalhes em indivíduos juvenis e adultos. É necessária a criação de medidas de regulamentação da pesca durante a temporada reprodutiva de baleias-francas em Santa Catarina, considerando as peculiaridades da pesca na região com o objetivo de minimizar as injúrias causadas às baleias e evitar prejuízos às comunidades pesqueiras locais.

  20. Coastal upwelling linked to toxic Pseudo-nitzschia australis blooms in Los Angeles coastal waters, 2005-2007

    KAUST Repository

    Schnetzer, Astrid

    2013-06-10

    Harmful algal blooms dominated by the diatom Pseudo-nitzschia spp. have become a perennial but variable event within surface waters near the greater Los Angeles area. Toxic blooms during spring seasons from 2005 to 2007 varied strongly in their overall toxicity and duration. Differences in bloom dynamics were linked to differences in storm-induced river discharge following episodic rain events and coastal upwelling, both major coastal processes that led to the injection of nutrients into coastal surface waters. Heavy river runoff during early 2005, a record-rainfall year, favored a phytoplankton community mainly comprised of algal taxa other than Pseudo-nitzschia. The spring bloom during 2005 was associated with low domoic acid surface concentrations and minor contributions of (mainly) P. delicatissima to the diatom assemblage. In contrast, highly toxic P. australis-dominated blooms during spring seasons of 2006 and 2007 were linked to strong upwelling events. River discharge quotas in 2006 and 2007, in contrast to 2005, fell well below annual averages for the region. Surface toxin levels were linked to colder, more saline (i.e. upwelled) water over the 3-year study, but no such consistent relationship between domoic acid levels and other physiochemical parameters, such as macronutrient concentrations or nutrient ratios, was observed. © The Author 2013. Published by Oxford University Press. All rights reserved.

  1. Effects of high salinity from desalination brine on growth, photosynthesis, water relations and osmolyte concentrations of seagrass Posidonia australis.

    Science.gov (United States)

    Cambridge, M L; Zavala-Perez, A; Cawthray, G R; Mondon, J; Kendrick, G A

    2017-02-15

    Highly saline brines from desalination plants expose seagrass communities to salt stress. We examined effects of raised salinity (46 and 54psu) compared with seawater controls (37psu) over 6weeks on the seagrass, Posidonia australis, growing in tanks with the aim of separating effects of salinity from other potentially deleterious components of brine and determining appropriate bioindicators. Plants survived exposures of 2-4weeks at 54psu, the maximum salinity of brine released from a nearby desalination plant. Salinity significantly reduced maximum quantum yield of PSII (chlorophyll a fluorescence emissions). Leaf water potential (Ψ w ) and osmotic potential (Ψ π ) were more negative at increased salinity, while turgor pressure (Ψ p ) was unaffected. Leaf concentrations of K + and Ca 2+ decreased, whereas concentrations of sugars (mainly sucrose) and amino acids increased. We recommend leaf osmolarity, ion, sugar and amino acid concentrations as bioindicators for salinity effects, associated with brine released in desalination plant outfalls. Copyright © 2016 Elsevier Ltd. All rights reserved.

  2. Effects of high salinity from desalination brine on growth, photosynthesis, water relations and osmolyte concentrations of seagrass Posidonia australis

    International Nuclear Information System (INIS)

    Cambridge, M L; Zavala-Perez, A; Cawthray, G R; Mondon, J; Kendrick, G A

    2017-01-01

    Highly saline brines from desalination plants expose seagrass communities to salt stress. We examined effects of raised salinity (46 and 54 psu) compared with seawater controls (37 psu) over 6 weeks on the seagrass, Posidonia australis, growing in tanks with the aim of separating effects of salinity from other potentially deleterious components of brine and determining appropriate bioindicators. Plants survived exposures of 2–4 weeks at 54 psu, the maximum salinity of brine released from a nearby desalination plant. Salinity significantly reduced maximum quantum yield of PSII (chlorophyll a fluorescence emissions). Leaf water potential (Ψ w ) and osmotic potential (Ψ π ) were more negative at increased salinity, while turgor pressure (Ψ p ) was unaffected. Leaf concentrations of K + and Ca 2+ decreased, whereas concentrations of sugars (mainly sucrose) and amino acids increased. We recommend leaf osmolarity, ion, sugar and amino acid concentrations as bioindicators for salinity effects, associated with brine released in desalination plant outfalls. - Highlights: • We separated salt effects of desalination brine from other deleterious components. • Sublethal salinity stress depended on both salinity increase and exposure time. • Very effective osmoregulation led to tolerance of short intervals of high salinity.

  3. Skull morphometrics of adult male Antartic fur seal, Arctocephalus gazella, and South American fur seal A. australis Morfometría craneana comparada de ejemplares machos adultos de lobo fino antártico, Arctocephalus gazella y lobo fino sudamericano, A. australis

    Directory of Open Access Journals (Sweden)

    Gustavo A. Daneri

    2005-09-01

    Full Text Available The skull morphometrics of adult male Antarctic fur seal, Arctocephalus gazella (Peters, 1875 and South American fur seal, A. australis (Zimmermann, 1783 were investigated using a collection of 45 and 38 skulls, respectively. Eighteen measurements were taken for each specimen. Comparative univariate and multivariate statistical analyses included standard statistics, one-way analysis of variance, principal component analysis and discriminant analysis. Individual variation was relatively high for some variables, as expressed by the coefficient of variation. Skulls of A. gazella were larger than those of A. australis for all but two variables: squamosal jugal suture and rostral length. Both species differed significantly as shown by both univariate and multivariate analyses. The discriminant function correctly classified all specimens. The standardized canonical coefficients showed that the variables which most contribute to the differentiation between species were, in decreasing order, the rostral length, palatal length, palatal width at postcanine 5 and braincase width. The present study corroborates that A. gazella and A. australis are phenotipically distinct species.Se estudió la morfometría craneana de especímenes machos adultos de lobo fino Antártico, Arctocephalus gazella (Peters, 1875 y lobo fino sudamericano, A. australis (Zimmermann, 1783, consultando una colección de 45 y 38 cráneos respectivamente. Para cada ejemplar se tomó un total de 18 medidas. El análisis comparativo univariado y multivariado incluyó estadística standard, análisis de varianza, análisis de componentes principales y análisis discriminante. La variación individual, según lo expresado por el coeficiente de variación, fue relativamente alta para algunas variables. Los cráneos de A. gazella fueron proporcionalmente más grandes que aquellos de A. australis para todas las variables estudiadas, excepto dos: longitud de la sutura escamoso-yugal y longitud

  4. Root parasitic plant Orobanche aegyptiaca and shoot parasitic plant Cuscuta australis obtained Brassicaceae-specific strictosidine synthase-like genes by horizontal gene transfer.

    Science.gov (United States)

    Zhang, Dale; Qi, Jinfeng; Yue, Jipei; Huang, Jinling; Sun, Ting; Li, Suoping; Wen, Jian-Fan; Hettenhausen, Christian; Wu, Jinsong; Wang, Lei; Zhuang, Huifu; Wu, Jianqiang; Sun, Guiling

    2014-01-13

    Besides gene duplication and de novo gene generation, horizontal gene transfer (HGT) is another important way of acquiring new genes. HGT may endow the recipients with novel phenotypic traits that are important for species evolution and adaption to new ecological niches. Parasitic systems expectedly allow the occurrence of HGT at relatively high frequencies due to their long-term physical contact. In plants, a number of HGT events have been reported between the organelles of parasites and the hosts, but HGT between host and parasite nuclear genomes has rarely been found. A thorough transcriptome screening revealed that a strictosidine synthase-like (SSL) gene in the root parasitic plant Orobanche aegyptiaca and the shoot parasitic plant Cuscuta australis showed much higher sequence similarities with those in Brassicaceae than with those in their close relatives, suggesting independent gene horizontal transfer events from Brassicaceae to these parasites. These findings were strongly supported by phylogenetic analysis and their identical unique amino acid residues and deletions. Intriguingly, the nucleus-located SSL genes in Brassicaceae belonged to a new member of SSL gene family, which were originated from gene duplication. The presence of introns indicated that the transfer occurred directly by DNA integration in both parasites. Furthermore, positive selection was detected in the foreign SSL gene in O. aegyptiaca but not in C. australis. The expression of the foreign SSL genes in these two parasitic plants was detected in multiple development stages and tissues, and the foreign SSL gene was induced after wounding treatment in C. australis stems. These data imply that the foreign genes may still retain certain functions in the recipient species. Our study strongly supports that parasitic plants can gain novel nuclear genes from distantly related host species by HGT and the foreign genes may execute certain functions in the new hosts.

  5. Transcriptome and Degradome of microRNAs and Their Targets in Response to Drought Stress in the Plants of a Diploid and Its Autotetraploid Paulownia australis.

    Directory of Open Access Journals (Sweden)

    Suyan Niu

    Full Text Available MicroRNAs (miRNAs are small, non-coding RNAs that play vital roles in plant growth, development, and stress response. Increasing numbers of studies aimed at discovering miRNAs and analyzing their functions in plants are being reported. In this study, we investigated the effect of drought stress on the expression of miRNAs and their targets in plants of a diploid and derived autotetraploid Paulownia australis. Four small RNA (sRNA libraries and four degradome libraries were constructed from diploid and autotetraploid P. australis plants treated with either 75% or 25% relative soil water content. A total of 33 conserved and 104 novel miRNAs (processing precision value > 0.1 were identified, and 125 target genes were identified for 36 of the miRNAs by using the degradome sequencing. Among the identified miRNAs, 54 and 68 were differentially expressed in diploid and autotetraploid plants under drought stress (25% relative soil water content, respectively. The expressions of miRNAs and target genes were also validated by quantitative real-time PCR. The results showed that the relative expression trends of the randomly selected miRNAs were similar to the trends predicted by Illumina sequencing. And the correlations between miRNAs and their target genes were also analyzed. Furthermore, the functional analysis showed that most of these miRNAs and target genes were associated with plant development and environmental stress response. This study provided molecular evidence for the possible involvement of certain miRNAs in the drought response and/or tolerance in P. australis, and certain level of differential expression between diploid and autotetraploid plants.

  6. Molecular phylogeny and taxonomic revision of the genus Wittrockiella (Pithophoraceae, Cladophorales), including the descriptions of W. australis sp. nov. and W. zosterae sp. nov.

    Science.gov (United States)

    Boedeker, Christian; O'Kelly, Charles J; West, John A; Hanyuda, Takeaki; Neale, Adele; Wakana, Isamu; Wilcox, Mike D; Karsten, Ulf; Zuccarello, Giuseppe C

    2017-06-01

    Wittrockiella is a small genus of filamentous green algae that occurs in habitats with reduced or fluctuating salinities. Many aspects of the basic biology of these algae are still unknown and the phylogenetic relationships within the genus have not been fully explored. We provide a phylogeny based on three ribosomal markers (ITS, LSU, and SSU rDNA) of the genus, including broad intraspecific sampling for W. lyallii and W. salina, recommendations for the use of existing names are made, and highlight aspects of their physiology and life cycle. Molecular data indicate that there are five species of Wittrockiella. Two new species, W. australis and W. zosterae, are described, both are endophytes. Although W. lyallii and W. salina can be identified morphologically, there are no diagnostic morphological characters to distinguish between W. amphibia, W. australis, and W. zosterae. A range of low molecular weight carbohydrates were analyzed but proved to not be taxonomically informative. The distribution range of W. salina is extended to the Northern Hemisphere as this species has been found in brackish lakes in Japan. Furthermore, it is shown that there are no grounds to recognize W. salina var. kraftii, which was described as an endemic variety from a freshwater habitat on Lord Howe Island, Australia. Culture experiments indicate that W. australis has a preference for growth in lower salinities over full seawater. For W. amphibia and W. zosterae, sexual reproduction is documented, and the split of these species is possibly attributable to polyploidization. © 2017 Phycological Society of America.

  7. Test Area C-80 Complex Final Range Environmental Assessment, Revision 1

    Science.gov (United States)

    2009-08-14

    to intense noise might contribute to the development and aggravation of stress -related conditions such as high blood pressure, coronary disease...produced by test and training missions and their associated expendables. Noise may directly inconvenience and/or stress humans and some wildlife...Sherman’s fox squirrel Sciuris niger shermani Phragmites Phragmites australis American alligator Alligator mississippiensis White cedar Chamaecyparis

  8. Final Environmental Assessment for Long-Term Vegetation Control for Eglin Air Force Base, Florida

    Science.gov (United States)

    2008-07-14

    domingensis Florida Black Bear Ursus americanus floridanus Phragmites Phragmites australis American Alligator Alligator mississippiensis White Cedar...Cont’d) Tulip Poplar Liriodendron tulipifera Indigo Snake Drymarchon corais Sweet Bay Magnolia Magnolia virginiana American Beaver Castor canadensis Red...Bay Persea borbonia Parula Warbler Parula americana Wetland and Riparian Ecological Association (Saltwater) Black Needle Rush Juncus roemerianus

  9. Surface pH changes suggest a role for H+/OH- channels in salinity response of Chara australis.

    Science.gov (United States)

    Absolonova, Marketa; Beilby, Mary J; Sommer, Aniela; Hoepflinger, Marion C; Foissner, Ilse

    2018-05-01

    To understand salt stress, the full impact of salinity on plant cell physiology has to be resolved. Electrical measurements suggest that salinity inhibits the proton pump and opens putative H + /OH - channels all over the cell surface of salt sensitive Chara australis (Beilby and Al Khazaaly 2009; Al Khazaaly and Beilby 2012). The channels open transiently at first, causing a characteristic noise in membrane potential difference (PD), and after longer exposure remain open with a typical current-voltage (I/V) profile, both abolished by the addition of 1 mM ZnCl 2 , the main known blocker of animal H + channels. The cells were imaged with confocal microscopy, using fluorescein isothiocyanate (FITC) coupled to dextran 70 to illuminate the pH changes outside the cell wall in artificial fresh water (AFW) and in saline medium. In the early saline exposure, we observed alkaline patches (bright fluorescent spots) appearing transiently in random spatial distribution. After longer exposure, some of the spots became fixed in space. Saline also abolished or diminished the pH banding pattern observed in the untreated control cells. ZnCl 2 suppressed the alkaline spot formation in saline and the pH banding pattern in AFW. The osmotic component of the saline stress did not produce transient bright spots or affect banding. The displacement of H + from the cell wall charges, the H + /OH - channel conductance/density, and self-organization are discussed. No homologies to animal H + channels were found. Salinity activation of the H + /OH - channels might contribute to saline response in roots of land plants and leaves of aquatic angiosperms.

  10. CHEMICAL AND PHYSICAL CONDITIONS IN MOLECULAR CLOUD CORE DC 000.4-19.5 (SL42) IN CORONA AUSTRALIS

    Energy Technology Data Exchange (ETDEWEB)

    Hardegree-Ullman, E.; Whittet, D. C. B. [New York Center for Astrobiology and Department of Physics, Applied Physics, and Astronomy, Rensselaer Polytechnic Institute, 110 Eighth Street, Troy, NY 12180 (United States); Harju, J. [Finnish Centre for Astronomy with ESO (FINCA), University of Turku, Vaeisaelaentie 20, FI-21500, Piikkioe (Finland); Juvela, M.; Sipilae, O. [Department of Physics, P.O. Box 64, FI-00014, University of Helsinki (Finland); Hotzel, S., E-mail: hardee@rpi.edu [Observatory, FI-00014, University of Helsinki (Finland)

    2013-01-20

    Chemical reactions in starless molecular clouds are heavily dependent on interactions between gas phase material and solid phase dust and ices. We have observed the abundance and distribution of molecular gases in the cold, starless core DC 000.4-19.5 (SL42) in Corona Australis using data from the Swedish ESO Submillimeter Telescope. We present column density maps determined from measurements of C{sup 18}O (J = 2-1, 1-0) and N{sub 2}H{sup +} (J = 1-0) emission features. Herschel data of the same region allow a direct comparison to the dust component of the cloud core and provide evidence for gas phase depletion of CO at the highest extinctions. The dust color temperature in the core calculated from Herschel maps ranges from roughly 10.7 to 14.0 K. This range agrees with the previous determinations from Infrared Space Observatory and Planck observations. The column density profile of the core can be fitted with a Plummer-like density distribution approaching n(r) {approx} r {sup -2} at large distances. The core structure deviates clearly from a critical Bonnor-Ebert sphere. Instead, the core appears to be gravitationally bound and to lack thermal and turbulent support against the pressure of the surrounding low-density material: it may therefore be in the process of slow contraction. We test two chemical models and find that a steady-state depletion model agrees with the observed C{sup 18}O column density profile and the observed N(C{sup 18}O) versus A{sub V} relationship.

  11. Anomalous colour in Neotropical mammals: a review with new records for Didelphis sp. (Didelphidae, Didelphimorphia and Arctocephalus australis (Otariidae, Carnivora

    Directory of Open Access Journals (Sweden)

    MSL. Abreu

    Full Text Available Anomalous colourations occur in many tropical vertebrates. However, they are considered rare in wild populations, with very few records for the majority of animal taxa. We report two new cases of anomalous colouration in mammals. Additionally, we compiled all published cases about anomalous pigmentation registered in Neotropical mammals, throughout a comprehensive review of peer reviewed articles between 1950 and 2010. Every record was classified as albinism, leucism, piebaldism or eventually as undetermined pigmentation. As results, we report the new record of a leucistic specimen of opossum (Didelphis sp. in southern Brazil, as well as a specimen of South American fur seal (Arctocephalus australis with piebaldism in Uruguay. We also found 31 scientific articles resulting in 23 records of albinism, 12 of leucism, 71 of piebaldism and 92 records classified as undetermined pigmentation. Anomalous colouration is apparently rare in small terrestrial mammals, but it is much more common in cetaceans and michrochiropterans. Out of these 198 records, 149 occurred in cetaceans and 30 in bats. The results related to cetaceans suggest that males and females with anomolous pigmentation are reproductively successful and as a consequence their frequencies are becoming higher in natural populations. In bats, this result can be related to the fact these animals orient themselves primarily through echolocation, and their refuges provide protection against light and predation. It is possible that anomalous colouration occurs more frequently in other Neotropical mammal orders, which were not formally reported. Therefore, we encourage researchers to publish these events in order to better understand this phenomenon that has a significant influence on animal survival.

  12. Organização e arquitetura microscópica do sistema tegumentar do Lobo-marinho-sul-americano (Arctocephalus australis, Zimmermann, 1783)

    OpenAIRE

    Ana Paula da Silva

    2008-01-01

    O lobo-marinho-sul-americano (Arctocephalus australis) membro da Ordem Carnivora, é comumente visto nos períodos de outono e inverno na costa brasileira do Rio Grande do Sul até o Rio de Janeiro, porém, sem colônias reprodutivas estabelecidas. A espécie pertence ao grupo dos pinípedes, carnívoros com membros em forma de nadadeiras que vivem em ambiente aquático e terrestre. Estudos sobre a morfologia da pele em pinípedes são raros e antigos, destes procederam muitos relatos divergentes, em vi...

  13. Resposta de plantas micropropagadas de abacaxizeiro à inoculação de bactérias diazotróficas em casa de vegetação Response of pineapple plantlets to inoculation with diazotrophic bacteria in greenhouse

    Directory of Open Access Journals (Sweden)

    Olmar Baller Weber

    2003-12-01

    Full Text Available O objetivo deste trabalho foi selecionar e avaliar bactérias diazotróficas isoladas de abacaxizeiro (Ananas comosus (L. Merril no desenvolvimento de cultivares micropropagadas da mesma espécie em casa de vegetação. Plantas da cultivar Perolera foram submetidas à inoculação com Asaia bogorensis (AB219 e cultivadas em tubetes, durante 145 dias, com as misturas: casca de arroz carbonizada, folha de carnaubeira triturada e vermicomposto; casca de arroz carbonizada, pó da casca do coco maduro e vermicomposto; casca de arroz carbonizada, vermiculita e vermicomposto. Plantas da cultivar Primavera receberam inóculos com o AB219 e bactérias relacionadas a Burkholderia cepacia (AB202 e AB213, enquanto plantas das cultivares Pérola e Smooth Cayenne receberam AB219 e AB213, sendo cultivadas, por 140 dias, em tubetes com a mistura de vermicomposto e vermiculita. A colonização dos abacaxizeiros pelas bactérias diazotróficas foi confirmada. As plantas da cultivar Perolera cresceram melhor em casca de arroz carbonizada, vermiculita e vermicomposto e responderam positivamente ao AB219. Já as plantas da cultivar Primavera não apresentaram resposta significativa à inoculação com AB219, AB202 e AB213. Houve incremento de 23,1% a 38,5% na matéria seca de raízes das plantas da cultivar Pérola na presença de AB213 e AB219, respectivamente. A presença de AB213 incrementou em 15,2% a matéria seca da parte aérea das plantas da cultivar Smooth Cayenne. Os resultados revelam a eficiência de bactérias diazotróficas na promoção do crescimento de abacaxizeiros.The objective of this work was to select and to evaluate isolates of diazotrophic bacteria associated to pineapple (Ananas comosus (L. Merril plants in the development of micropropagated plantlets of cultivars pineapple in greenhouse. Plantlets from the Perolera cultivar have been submitted to inoculation with Asaia bogorensis (AB219 and were cultivated during 145 days on the following

  14. Single cell gel electrophoresis as a tool to assess genetic damage in Heleobia cf. australis (Mollusca: Gastropoda as sentinel for industrial and domestic pollution in Montevideo bay (Uruguay

    Directory of Open Access Journals (Sweden)

    Silvia Villar

    2015-09-01

    Full Text Available AbstractThe knowledge of the extent of DNA damage in aquatic organisms in polluted areas is an important issue because contamination may alter their health at sublethal levels. Although molluscs have been widely used to monitor water pollution, there are no records of in vivo genotoxicity studies. Heleobia cf. australis, is distributed in almost all Uruguayan coastal ecosystems, including highly polluted sites. The comet assay is a damage genetic biomarker based on the migration of negatively charged DNA fragments produced by mutagenic agents in individual cells. Live individuals were collected in the Montevideo Bay (impacted area and Laguna Garzón (control to analyze the presence of mutagenic agents in the former site through comet assay. Cells from organisms of the impacted area showed significantly higher levels of genetic damage than those obtained in the control population, measured by percentage of DNA in the tail. Although preliminary, this approach supports the idea that H. cf. australis could be used as a sentinel to evaluate the presence of mutagenic agents in estuarine environments, alerting to the impact of contamination in its early stages.

  15. Extracts from Cladiella australis, Clavularia viridis and Klyxum simplex (Soft Corals are Capable of Inhibiting the Growth of Human Oral Squamous Cell Carcinoma Cells

    Directory of Open Access Journals (Sweden)

    Guey-Horng Wang

    2008-11-01

    Full Text Available Many biomedical products have already been obtained from marine organisms. In order to search more therapeutic drugs against cancer, this study demonstrates the cytotoxicity effects of Cladiella australis, Clavularia viridis and Klyxum simplex extractson human oral squamous cell carcinoma (SCC4, SCC9 and SCC25 cells using cell adhesion and cell viability assay. The morphological alterations in SCCs cells after treatment with three extracts, such as typical nuclear condensation, nuclear fragmentation and apoptotic bodies of cells were demonstrated by Hoechst stain. Flow cytometry indicated that three extracts sensitized SCC25 cells in the G0/G1 and S-G2/M phases with a concomitant significantly increased sub-G1 fraction, indicating cell death by apoptosis. This apoptosis process was accompanied by activation of caspase-3 expression after SCC25 cells were treated with three extracts. Thereby, it is possible that extracts of C. australis, C. viridis and K. simplex cause apoptosis of SCCs and warrant further research investigating the possible anti-oral cancer compounds in these soft corals.

  16. Intestinal helminth fauna of the South American sea lion Otaria flavescens and fur seal Arctocephalus australis from northern Patagonia, Argentina.

    Science.gov (United States)

    Hernández-Orts, J S; Montero, F E; Juan-García, A; García, N A; Crespo, E A; Raga, J A; Aznar, F J

    2013-09-01

    We report on the intestinal helminth fauna of 56 South American sea lions, Otaria flavescens, and 5 South American fur seals, Arctocephalus australis, from northern Patagonia, Argentina. A total of 97,325 helminth specimens were collected from sea lions. Gravid individuals were represented by 6 species of parasites: 1 digenean (Ascocotyle (Ascocotyle) patagoniensis), 1 cestode (Diphyllobothrium spp.), 3 nematodes (Uncinaria hamiltoni, Contracaecum ogmorhini s.s., Pseudoterranova cattani) and 1 acanthocephalan (Corynosoma australe). In addition, third-stage larvae of 2 nematodes (Contracaecum sp. and Anisakis sp. type I) and 3 juvenile acanthocephalans (Andracantha sp., Profilicollis chasmagnathi and Corynosoma cetaceum) were also collected. Andracantha sp., C. ogmorhini s.s. and P. chasmagnathi represent new host records. A total of 1516 helminth specimens were collected from fur seals. Gravid individuals were represented by three species of parasites, namely, Diphyllobothrium spp., C. ogmorhini s.s. and C. australe. In addition, larvae of Contracaecum sp. and P. cattani, juveniles of C. cetaceum and immature cestodes (Tetrabothriidae gen. sp.) were also collected. Corynosoma australe was the most prevalent and abundant parasite in both hosts, accounting for >90% of all specimens. Sea lions and furs seals from northern Patagonia harbour the intestinal helminth communities that could be predicted for otariids, i.e. the combination of species of the genera Corynosoma, Diphyllobothrium, Pseudoterranova, Contracaecum and, in pups, Uncinaria. Additionally, both species of otariid are apparently unsuitable hosts (i.e. non-hosts) for as many as five parasite taxa. The inclusion or exclusion of these species affects estimation of species richness at both component community (11 versus 6 species in sea lions; 7 versus 3 species in fur seals) and infracommunity (mean: 3.1 versus 2.6 in sea lions; 2.2 versus 1.7 species) levels. Information about the reproductive status of

  17. THE SPITZER SURVEY OF INTERSTELLAR CLOUDS IN THE GOULD BELT. III. A MULTI-WAVELENGTH VIEW OF CORONA AUSTRALIS

    International Nuclear Information System (INIS)

    Peterson, Dawn E.; Bourke, Tyler L.; Forbrich, Jan; Patten, Brian M.; Caratti o Garatti, Alessio; Gutermuth, Robert A.; Joergensen, Jes K.; Allen, Lori E.; Dunham, Michael M.; Harvey, Paul M.; Evans, Neal J.; MerIn, Bruno; Chapman, Nicholas L.; Cieza, Lucas A.; Huard, Tracy L.; Knez, Claudia; Prager, Brian

    2011-01-01

    We present Spitzer Space Telescope IRAC and MIPS observations of a 0.85 deg 2 field including the Corona Australis (CrA) star-forming region. At a distance of 130 pc, CrA is one of the closest regions known to be actively forming stars, particularly within its embedded association, the Coronet. Using the Spitzer data, we identify 51 young stellar objects (YSOs) in CrA which include sources in the well-studied Coronet cluster as well as sources distributed throughout the molecular cloud. Twelve of the YSOs discussed are new candidates, one of which is located in the Coronet. Known YSOs retrieved from the literature are also added to the list, and a total of 116 candidate YSOs in CrA are compiled. Based on these YSO candidates, the star formation rate is computed to be 12 M sun Myr -1 , similar to that of the Lupus clouds. A clustering analysis was also performed, finding that the main cluster core, consisting of 68 members, is elongated (having an aspect ratio of 2.36), with a circular radius of 0.59 pc and mean surface density of 150 pc -2 . In addition, we analyze outflows and jets in CrA by means of new CO and H 2 data. We present 1.3 mm interferometric continuum observations made with the Submillimeter Array (SMA) covering R CrA, IRS 5, IRS 7, and IRAS 18595-3712 (IRAS 32). We also present multi-epoch H 2 maps and detect jets and outflows, study their proper motions, and identify exciting sources. The Spitzer and ISAAC/VLT observations of IRAS 32 show a bipolar precessing jet, which drives a CO(2-1) outflow detected in the SMA observations. There is also clear evidence for a parsec-scale precessing outflow, which is east-west oriented and originates in the SMA 2 region and likely driven by SMA 2 or IRS 7A.

  18. Warm gas towards young stellar objects in Corona Australis. Herschel/PACS observations from the DIGIT key programme

    Science.gov (United States)

    Lindberg, Johan E.; Jørgensen, Jes K.; Green, Joel D.; Herczeg, Gregory J.; Dionatos, Odysseas; Evans, Neal J.; Karska, Agata; Wampfler, Susanne F.

    2014-05-01

    Context. The effects of external irradiation on the chemistry and physics in the protostellar envelope around low-mass young stellar objects are poorly understood. The Corona Australis star-forming region contains the R CrA dark cloud, comprising several low-mass protostellar cores irradiated by an intermediate-mass young star. Aims: We study the effects of the irradiation coming from the young luminous Herbig Be star R CrA on the warm gas and dust in a group of low-mass young stellar objects. Methods: Herschel/PACS far-infrared datacubes of two low-mass star-forming regions in the R CrA dark cloud are presented. The distributions of CO, OH, H2O, [C ii], [O i], and continuum emission are investigated. We have developed a deconvolution algorithm which we use to deconvolve the maps, separating the point-source emission from the extended emission. We also construct rotational diagrams of the molecular species. Results: By deconvolution of the Herschel data, we find large-scale (several thousand AU) dust continuum and spectral line emission not associated with the point sources. Similar rotational temperatures are found for the warm CO (282 ± 4 K), hot CO (890 ± 84 K), OH (79 ± 4 K), and H2O (197 ± 7 K) emission in the point sources and the extended emission. The rotational temperatures are also similar to those found in other more isolated cores. The extended dust continuum emission is found in two ridges similar in extent and temperature to molecular millimetre emission, indicative of external heating from the Herbig Be star R CrA. Conclusions: Our results show that nearby luminous stars do not increase the molecular excitation temperatures of the warm gas around young stellar objects (YSOs). However, the emission from photodissociation products of H2O, such as OH and O, is enhanced in the warm gas associated with these protostars and their surroundings compared to similar objects not subjected to external irradiation. Table 9 and appendices are available in

  19. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from AURORA AUSTRALIS in the Indian Ocean, South Pacific Ocean and Southern Oceans from 2011-01-04 to 2011-02-06 (NCEI Accession 0143947)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0143947 includes discrete sample and profile data collected from AURORA AUSTRALIS in the Indian Ocean, South Pacific Ocean and Southern Oceans (>...

  20. Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using Alkalinity titrator, CTD and other instruments from the AURORA AUSTRALIS in the Indian Ocean from 2008-03-22 to 2008-04-17 (NODC Accession 0109900)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0109900 includes biological, chemical, discrete sample, physical and profile data collected from AURORA AUSTRALIS in the Indian Ocean from 2008-03-22...

  1. Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using Alkalinity titrator, CTD and other instruments from AURORA AUSTRALIS in the Indian Ocean and Southern Oceans from 2006-01-02 to 2006-03-12 (NODC Accession 0109922)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0109922 includes chemical, discrete sample, optical, physical and profile data collected from AURORA AUSTRALIS in the Indian Ocean and Southern Oceans...

  2. Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the AURORA AUSTRALIS in the Great Australian Bight and Indian Ocean from 1998-02-28 to 1998-04-01 (NODC Accession 0115154)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115154 includes chemical, discrete sample, physical and profile data collected from AURORA AUSTRALIS in the Great Australian Bight and Indian Ocean...

  3. Partial pressure (or fugacity) of carbon dioxide, dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, PAR Sensor and other instruments from AURORA AUSTRALIS in the Indian Ocean and Southern Oceans from 2016-01-11 to 2016-03-15 (NCEI Accession 0163181)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0163181 includes chemical, discrete sample, optical, physical and profile data collected from AURORA AUSTRALIS in the Indian Ocean and Southern Oceans...

  4. Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using Alkalinity titrator, CTD and other instruments from AURORA AUSTRALIS in the Indian Ocean and Southern Oceans from 2004-12-23 to 2005-02-17 (NODC Accession 0108076)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0108076 includes discrete sample and profile data collected from AURORA AUSTRALIS in the Indian Ocean and Southern Oceans (> 60 degrees South) from...

  5. Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the AURORA AUSTRALIS in the Indian Ocean and South Pacific Ocean from 1994-12-13 to 1995-02-01 (NODC Accession 0115020)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115020 includes chemical, discrete sample, physical and profile data collected from AURORA AUSTRALIS in the Indian Ocean and South Pacific Ocean from...

  6. Dissolved inorganic carbon, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from AURORA AUSTRALIS in the Indian Ocean, South Pacific Ocean and others from 1993-04-04 to 1993-05-09 (NODC Accession 0115004)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0115004 includes chemical, discrete sample, physical and profile data collected from AURORA AUSTRALIS in the Indian Ocean, South Pacific Ocean,...

  7. Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using Alkalinity titrator, CTD and other instruments from the AURORA AUSTRALIS in the Indian Ocean from 2001-10-29 to 2001-12-13 (NODC Accession 0108158)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0108158 includes chemical, discrete sample, physical and profile data collected from AURORA AUSTRALIS in the Indian Ocean from 2001-10-29 to...

  8. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from AURORA AUSTRALIS in the Indian Ocean and Southern Oceans from 1995-07-17 to 1995-09-02 (NCEI Accession 0144339)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0144339 includes discrete sample and profile data collected from AURORA AUSTRALIS in the Indian Ocean and Southern Oceans (> 60 degrees South) from...

  9. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, PAR Sensor and other instruments from AURORA AUSTRALIS in the Indian Ocean, South Pacific Ocean and Southern Oceans from 2007-12-16 to 2008-01-27 (NCEI Accession 0143932)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0143932 includes discrete sample and profile data collected from AURORA AUSTRALIS in the Indian Ocean, South Pacific Ocean and Southern Oceans (>...

  10. Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from underway - surface observations using Barometric pressure sensor, Carbon dioxide (CO2) gas analyzer and other instruments from the AURORA AUSTRALIS in the Great Australian Bight, Indian Ocean and others from 1992-10-19 to 2001-12-12 (NODC Accession 0115153)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115153 includes chemical, meteorological, physical and underway - surface data collected from AURORA AUSTRALIS in the Great Australian Bight, Indian...

  11. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, PAR Sensor and other instruments from AURORA AUSTRALIS in the Indian Ocean and Southern Oceans from 2012-01-05 to 2012-02-12 (NCEI Accession 0143949)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0143949 includes discrete sample and profile data collected from AURORA AUSTRALIS in the Indian Ocean and Southern Oceans (> 60 degrees South) from...

  12. Influência de porta-enxertos na resistência de mudas de cajueiro ao estresse salino Influence of rootstocks on the resistance of cashew plantlets to salt stress

    Directory of Open Access Journals (Sweden)

    Sérgio Luiz Ferreira-Silva

    2009-04-01

    Full Text Available O objetivo deste trabalho foi avaliar a influência de porta-enxertos na resistência de mudas de cajueiro (Anacardium occidentale L. à salinidade. As mudas foram obtidas pela enxertia do clone BRS 226 sobre os porta-enxertos CAPI 4, CCP 09 e BRS 226. Foram expostas a meio hidropônico sem NaCl (controle ou com NaCl 200 mM (tratamento salino, sob condições controladas de temperatura, umidade e luminosidade, durante 12 dias. O delineamento foi o inteiramente casualizado, em esquema fatorial 3x2 (três combinações de enxerto/porta-enxerto e duas concentrações de NaCl, com quatro repetições. Foram determinados a concentração de Na+, Cl-, K+ e solutos orgânicos e os sintomas visuais de toxicidade nas folhas. Os conteúdos de Na+ e Cl-, a relação K+/Na+ e as concentrações de aminoácidos e de prolina livres nas folhas tiveram relação direta com os sintomas visuais de toxicidade. Os porta-enxertos CAPI 4, CCP 09 e BRS 226 foram classificados como sensível, intermediário e resistente à salinidade elevada, respectivamente. Essa variação foi decorrente da influência do porta-enxerto na partição do Na+ e do Cl-.The aim of this work was to evaluate the influence of cashew (Anacardium occidentale L. rootstocks on salt resistance in grafted plantlets. The plantlets were obtained by grafting the BRS 226 scion on CAPI 4, CCP 09 and BRS 226 rootstocks, and were exposed to nutrient solution without NaCl (control or with 200 mM NaCl (salt treatment in hydroponics under controlled temperature, humidity, and luminosity conditions during 12 days. The experimental design was a completely randomized in factorial arrangement 3x2 (three scion/rootstock combinations, two NaCl concentrations with four replicates. Na+, Cl-, K+ and organic solute concentrations and toxicity symptoms on leaves were determined. Na+ and Cl- contents, the K+/Na+ ratio and the free amino acid and proline concentrations in leaves were directly related to the visual

  13. Germinação de sementes e crescimento de plântulas de soja (Glycine max L. Merrill sob cobertura vegetal = Soybean (Glycine max L. Merrill seed germination and plantlet growth under vegetation cover

    Directory of Open Access Journals (Sweden)

    Lúcia Helena Pereira Nóbrega

    2009-07-01

    Full Text Available A eficiência do sistema de rotação de cultura requer, entre outrostratamentos, a escolha adequada das espécies a serem instaladas. Culturas vegetais podem apresentar compostos aleloquímicos, os quais são liberados por meio de pelos radiculares, sementes, raízes, colmos e folhas, em quantidades variáveis, capazes de interferir nas culturas subsequentes, comprometendo a produção. Assim, este estudoanalisou o potencial alelopático de aveia-preta (Avena strigosa Schreb (AP, nabo forrageiro (Raphanus sativus L. (NF, ervilhaca (Vicia sativa L. (ER, azevém (Lolium multiflorum Lam. (AZ e consórcio (CO - AP+ER+NF na germinação de sementes e crescimento de plântulas de soja. O experimento foi em laboratório, com substrato deareia, onde foram cultivadas plantas de cobertura, por 30 dias, mantendo os restos radiculares das plantas com e sem restos de parte aérea. Observou-se redução na emergência de plântulas de soja sob CO, AZ e AP. O índice de velocidade de emergência (IVE, a porcentagem de emergência em areia (EA e a massa fresca de hipocótilo (MFH foram afetados negativamente pelas plantas de cobertura.In order to be efficient, the crop rotation system requires, among other factors, an adequate choice of species to be installed. Vegetalcultures can feature allelochemical compounds, released by root hairs, roots, stems and leaves in variable amounts, which are able to interfere on subsequent cultures, as well as cause a delay in their production. Thus, this study analyzed the allelopathic potentialof black oat (Avena strigosa Schreb (BO, forage turnip (Raphanus sativus L. (FT, vetch (Vicia sativa L. (V, ryegrass (Lolium multiflorum Lam. (RG and consortium (CO - BO+V+FT on soybean seed germination and plantlet growth. This trial was carried out in a laboratory, with sand substrate, where coverage plants were grown, during 30 days. The remaining root portions of plants were kept with and without the remaining aerial part portions

  14. Development of an Efficient Protein Extraction Method Compatible with LC-MS/MS for Proteome Mapping in Two Australian Seagrasses Zostera muelleri and Posidonia australis

    Directory of Open Access Journals (Sweden)

    Zhijian Jiang

    2017-08-01

    Full Text Available The availability of the first complete genome sequence of the marine flowering plant Zostera marina (commonly known as seagrass in early 2016, is expected to significantly raise the impact of seagrass proteomics. Seagrasses are marine ecosystem engineers that are currently declining worldwide at an alarming rate due to both natural and anthropogenic disturbances. Seagrasses (especially species of the genus Zostera are compromised for proteomic studies primarily due to the lack of efficient protein extraction methods because of their recalcitrant cell wall which is rich in complex polysaccharides and a high abundance of secondary metabolites in their cells. In the present study, three protein extraction methods that are commonly used in plant proteomics i.e., phenol (P; trichloroacetic acid/acetone/SDS/phenol (TASP; and borax/polyvinyl-polypyrrolidone/phenol (BPP extraction, were evaluated quantitatively and qualitatively based on two dimensional isoelectric focusing (2D-IEF maps and LC-MS/MS analysis using the two most abundant Australian seagrass species, namely Zostera muelleri and Posidonia australis. All three tested methods produced high quality protein extracts with excellent 2D-IEF maps in P. australis. However, the BPP method produces better results in Z. muelleri compared to TASP and P. Therefore, we further modified the BPP method (M-BPP by homogenizing the tissue in a modified protein extraction buffer containing both ionic and non-ionic detergents (0.5% SDS; 1.5% Triton X-100, 2% PVPP and protease inhibitors. Further, the extracted proteins were solubilized in 0.5% of zwitterionic detergent (C7BzO instead of 4% CHAPS. This slight modification to the BPP method resulted in a higher protein yield, and good quality 2-DE maps with a higher number of protein spots in both the tested seagrasses. Further, the M-BPP method was successfully utilized in western-blot analysis of phosphoenolpyruvate carboxylase (PEPC—a key enzyme for carbon

  15. Comparative evaluation of the sperm characteristics and morphology of adult Wistar rats fed either low or normal protein-energy diets and orally dosed with aqueous Cuscuta australis extracts.

    Science.gov (United States)

    Omirinde, J O; Ozegbe, P C; Oyeyemi, M O

    2014-06-19

    Cuscuta australis (C. australis) seed and stem are commonly used as dietary supplements in a maize-meal, "Ogi", by the local population for the management of male and female reproductive dysfunctions. This study, as a part of on-going efforts, therefore, evaluated and compared the effects of Low Protein-energy (LP) and Normal Protein-energy (NP) diets on the sperm morphology and characteristics of adult Wistar rats orally dosed aqueous extracts of C. australis seed (LPSE and NPSE) and stem (LPST and NPST), 300 mg of extract/kg body weight of rat/day, for seven days. The control groups (LPWA and NPWA) received vehicle, water. Live-dead ratio and percentage of sperms with curved tail were significantly decreased (p<0.01) in the NPST relative to the NPWA, LPWA, LPST, NPSE and LPSE. Total abnormal sperm counts, acephalic sperms and tailless head sperms were significantly decreased (p<0.001, p<0.05 and p<0.001, respectively) in the LPST and NPST relative to LPSE, NPSE, LPWA and NPWA. The LPSE, LPST and NPST showed significantly decreased (p<0.05) percentages of sperms with either bent mid-piece or curved mid-piece relative to the LPWA. Significantly decreased (p<0.05) percentage of sperms with curved mid-piece was also observed in the NPSE relative to LPWA. Protein-energy diet significantly influenced (at least p<0.05) the effect of each extract on sperm motility and percentage of sperms with curved tail. Stem extract significantly decreased (p<0.01) the percentages of acephalic sperms and tailless head sperms. Diet-stem extract interaction significantly influenced (p<0.05) live-dead ratio. Our data suggest that orally administered aqueous extracts of C. australis generally enhanced the sperm morphology and characteristics of the male Wistar rat and that the stem extract maintained sperm morphology better than the seed extract. It also showed that the stem extract decreased live-dead ratio and that the efficacy of orally administered aqueous C. australis stem extract

  16. Livestock as a potential biological control agent for an invasive wetland plant

    Directory of Open Access Journals (Sweden)

    Brian R. Silliman

    2014-09-01

    Full Text Available Invasive species threaten biodiversity and incur costs exceeding billions of US$. Eradication efforts, however, are nearly always unsuccessful. Throughout much of North America, land managers have used expensive, and ultimately ineffective, techniques to combat invasive Phragmites australis in marshes. Here, we reveal that Phragmites may potentially be controlled by employing an affordable measure from its native European range: livestock grazing. Experimental field tests demonstrate that rotational goat grazing (where goats have no choice but to graze Phragmites can reduce Phragmites cover from 100 to 20% and that cows and horses also readily consume this plant. These results, combined with the fact that Europeans have suppressed Phragmites through seasonal livestock grazing for 6,000 years, suggest Phragmites management can shift to include more economical and effective top-down control strategies. More generally, these findings support an emerging paradigm shift in conservation from high-cost eradication to economically sustainable control of dominant invasive species.

  17. Study of the effects of Cobalt 60 gamma radiations on the seeds of two textile hibiscus species. Influence of seed water content on their radiosensitivity. Mutagenic effects on two plantlet generation

    International Nuclear Information System (INIS)

    Mahama, Adamou.

    1980-10-01

    This work on textile hibiscus was undertaken for two reasons: to contribute by physical mutagenesis methods to the genetic improvement of these species and especially to obtain the genetic diversity which is seriously lacking in the species sabdariffa var. altissima; to add to research on the effects of mutagenic seed treatments and thus to help towards a better definition of optimum seed irradiation conditions. These two purposes have been fulfilled. The main results may be summed up as follows. - Concerning the improvement of textile hibiscus. Many strains with various new features were selected at the second generation and may lead either to new varieties or at least to interesting precursors for selection programmes. In the second generation derived from Hibiscus saddariffa var. THS 22 some plants were observed with one or more of the following characteristics specific to the edible form: ramified bearing - fleshy calix - smooth leaf. - Concerning the study of mutagenic seed treatment effects. Our experiments showed up the importance of the seed water content and its effect on the response of first-generation plantlets to cobalt 60 γ-ray treatments [fr

  18. Morpho-physiological changes in pineapple plantlets [Ananas comosus (L. merr.] during acclimatization Mudanças morfofisiológicas em plântulas de abacaxi [Ananas comosus (L. Merr.] durante a aclimatização

    Directory of Open Access Journals (Sweden)

    Ariel Villalobo

    2012-12-01

    Full Text Available Pineapple producing countries lack good quality propagation material to expand cultivars into new areas. Previously, research protocols may increase the offer of high quality plantlets and speed the introduction of new pineapple cultivars. The present work is to evaluate the morpho-physiological changes in plantlets of pineapple [Ananas comosus (L. Merr. 'MD-2'] during the acclimatization phase. Plantlets were acclimatized under 80% relative humidity, 25.5 °C temperature and photosynthetic photon flux of 400-500 µmol m-2 s-1 as average for 45 d under natural photoperiods. All measurements (plant length, number of leaves and roots, fresh weight, width and length of leaf 'D', net photosynthesis and total transpiration rate were carried out at the end of in vitro rooting phase coincident with 0 d of acclimatization and at 15, 30 and 45 d thereafter. Photosynthetic activity of in vitro plantlets did not increase during the first 30 d of the acclimatization phase. After 30 d, photosynthetic activity ranged from 5.72 to 9.36 µmol CO2 m-2 s-1 while total transpiration ranged from 6.0 to 1.42 mmol H2O m-2 s-1. During the first 30 days there were no significant differences in number of leaves, length or width of the longest ('D' leaf (cm or plant length (cm. However, after 45 days plant fresh weight (g, length and width of the 'D' leaf (cm and root number all increased significantly, while transpiration (mmol H2O m-2 s-1 declined. There were small but significant decreases in chlorophyll a and b (µg g-1 mf.. Increased photosynthetic activity after 30 d shows that the increase in light intensity and the reduction of relative humidity during acclimatization did not constitute inhibitory factors.Os países produtores do abacaxi precisam de mudas de boa qualidade para a introdução de novas culturas nas áreas de cultivo. Os protocolos de pesquisa podem aumentar a oferta de mudas de alta qualidade e acelerar a introdução de novas culturas do abacaxi

  19. Indução de metabólitos secundários em plântulas de Hypericum brasiliense Choisy crescendo in vitro Induction of secondary metabolites in plantlets of Hypericum brasiliense Choisy in vitro

    Directory of Open Access Journals (Sweden)

    Mariella Araújo Luna Velloso

    2009-01-01

    Full Text Available A produção de rutina, quercetina, 1,5-diidroxixantona e ácido betulínico foi investigada em plântulas de H. brasiliense crescendo in vitro, sob a influência de ácido salicílico, polietilenoglicol, NaCl, 24-epibrassinolídeo, benzotiadiazole (BION, metiljasmonato e concentrações aumentadas de boro e nitrogênio no meio líquido de cultura. As avaliações foram feitas após 5 e 10 dias do início dos tratamentos. Os maiores aumentos de conteúdo foram observados com quercetina para boro e ácido salicílico aos 5 dias, e 24-epibrassinolídeo e BION aos 10 dias.The production of rutin, quercetin, 1,5-dihydroxyxanthone and betulinic acid was investigated in plantlets of H. brasieliense in vitro, and exposed to salycilic acid, poliethylene glycol, NaCl, 24-epibrassinolide, benzothiadiazole (BION, methyljasmonate and increased concentrations of boron and nitrogen in the liquid culture medium. Evaluations of the contents were carried out after 5 and 10 days of treatments. The highest increase was observed in quercetin in the salycilic acid and B treatments after 5 days of exposure, and in 24-epibrassinolide and BION after 10 days.

  20. Quince años de sucesión después de corta experimental en brezales de Erica australis L. en la provincia de León (España

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    Calvo, L.

    2000-12-01

    Full Text Available The response of the woody species to experimental cutting was studied for a period of fifteen years in a shrub community of NW Spain. This treatment represents the disturbance most frequently imposed by humans on these shrub communities throughout history. The dominant species, Erica australis, influences the regeneration patterns of the rest of the species which make up the community. There is a significant increase in the cover values of the woody species until the fourth year and of the herbaceous ones until the third year. Since then Erica australis attains the spatial occupancy and cover values it originally had, removing the herbaceous species and negatively influencing the growth of some woody ones like Halimium umbellatum and H. alyssoides. Both Erica australis and Genistella tridentata sprout after cutting. Arctostaphylos uva-ursi does not recover. Halimium alyssoides, H. umbellatum. Erica umbellata and Calluna vulgaris regenerate by germination. These shrubland communities have a high degree of resilience due to the strong sprouting potential of the component species.

    [fr]
    L'étude porte sur la régénération des espèces ligneuses après coupe, sur une durée de 15 ans, dans une lande dominée par Erica australis. Ce traitement experimental est l'un des plus fréquemment utilisés dans ce type de commimautés. L'espèce dominante Erica australis agit sur les types de régénération des autres espèces. On observe un accroisement significatif des taux de couverture pour les espèces ligneuses jusqu'au 4ème. année et jusqu'au Sème, année pour les herbacées. À partir de ce moment. Erica australis retrouve les valeurs d' origine, en limitant les taux des herbacées et en diminuant la croissance de quelques espèces ligneuses comme Halimium umbellatum et H. alyssoides. La régénération par rejet est le m

  1. Circadian changes in endogenous concentrations of indole-3-acetic acid, melatonin, serotonin, abscisic acid and jasmonic acid in Characeae (Chara australis Brown).

    Science.gov (United States)

    Beilby, Mary J; Turi, Christina E; Baker, Teesha C; Tymm, Fiona Jm; Murch, Susan J

    2015-01-01

    Giant-celled Characeae (Chara australis Brown), grown for 4 months on 12/12 hr day/night cycle and summer/autumn temperatures, exhibited distinct concentration maxima in auxin (indole-3-acetic acid; IAA), melatonin and serotonin about 4 hr after subjective daybreak. These concentration peaks persisted after 3 day pretreatment in continuous darkness: confirming a circadian rhythm, rather than a response to "light on." The plants pretreated for 3 d in continuous light exhibited several large IAA concentration maxima throughout the 24 hr. The melatonin and serotonin concentrations decreased and were less synchronized with IAA. Chara plants grown on 9/15 hr day/night cycle for 4 months and winter/spring temperatures contained much smaller concentrations of IAA, melatonin and serotonin. The IAA concentration maxima were observed in subjective dark phase. Serotonin concentration peaks were weakly correlated with those of IAA. Melatonin concentration was low and mostly independent of circadian cycle. The "dark" IAA concentration peaks persisted in plants treated for 3 d in the dark. The plants pretreated for 3 d in the light again developed more IAA concentration peaks. In this case the concentration maxima in melatonin and serotonin became more synchronous with those in IAA. The abscisic acid (ABA) and jasmonic acid (JA) concentrations were also measured in plants on winter regime. The ABA concentration did not exhibit circadian pattern, while JA concentration peaks were out of phase with those of IAA. The data are discussed in terms of crosstalk between metabolic pathways.

  2. In vitro apoptotic effects of methanol extracts of Dianthus chinensis and Acalypha australis L. targeting specificity protein 1 in human oral cancer cells.

    Science.gov (United States)

    Shin, Ji-Ae; Kim, Jae-Jin; Choi, Eun-Sun; Shim, Jung-Hyun; Ryu, Mi Heon; Kwon, Ki Han; Park, Hee-Min; Seo, Jin-Young; Lee, Soo-Yeon; Lim, Do-Won; Cho, Nam-Pyo; Cho, Sung-Dae

    2013-07-01

    The aims of this study were to evaluate the apoptotic activities and molecular mechanisms of methanol extracts of Dianthus chinensis (MEDC) and Acalypha australis L. (MEAL) in human oral cancer cells. The apoptotic effects and related molecular mechanisms of MEDC and MEAL on oral cancer cells were evaluated using MTS assay, DAPI staining, immunostaining, Western blotting, and reverse transcriptase-polymerase chain reaction. Sp1 was overexpressed in oral tumor tissues compared with normal oral mucosa. Downregulation of Sp1 inhibited the growth of SCC-15 and YD-15 oral cancer cells. MEDC and MEAL inhibited cell growth and induced apoptosis in both cell lines by decreasing the expression of Sp1. In addition, treatment of cells with MEDC and MEAL decreased Mcl-1 expression, which is a downstream target of Sp1. Our results indicate that MEDC and MEAL are bioactive natural products that can potentially induce apoptosis of tumor cells that overexpress the Sp1 protein. Copyright © 2012 Wiley Periodicals, Inc.

  3. In situ O2 dynamics in submerged Isoetes australis:

    DEFF Research Database (Denmark)

    Pedersen, Ole; Pulido Pérez, Cristina; Rich, S.M.

    2011-01-01

    , and the potential importance of the achlorophyllous leaf bases to underwater net photosynthesis (PN) and radial O2 loss to sediments is highlighted. O2 microelectrodes were used in situ to monitor pO2 in leaves, shallow sediments, and water in four vernal pools. The role of the achlorophyllous leaf bases in gas...... exchange was evaluated in laboratory studies of underwater PN, loss of tissue water, radial O2 loss, and light microscopy. Tissue and sediment pO2 showed large diurnal amplitudes and internal O2 was more similar to sediment pO2 than water pO2. In early afternoon, sediment pO2 was often higher than tissue p......A unique type of vernal pool are those formed on granite outcrops, as the substrate prevents percolation so that water accumulates in depressions when precipitation exceeds evaporation. The O2 dynamics of small, shallow vernal pools with dense populations of Isoetes australis were studied in situ...

  4. Evaluation of the effect of an extract of sabugueiro (Sambucus australis) on the labeling of blood constituents with technetium-99m

    International Nuclear Information System (INIS)

    Ribeiro, Camila Godinho; Rebello, Bernardo Machado; Neves, Rosane de Figueiredo; Santos-Filho, Sebastiao David; Fonseca, Adenilson de Souza da; Medeiros, Aldo da Cunha; Bernardo-Filho, Mario; Catanho, Maria Teresa Jansem de Almeida

    2007-01-01

    Sambucus australis (sabugueiro) has been used to treat inflammatory and rheumatologic disorders. Blood constituents labeled with technetium-99m (99mTc) have been used in nuclear medicine to obtain diagnostic images. The aim of this work was to evaluate the effect of a sabugueiro extract on the labeling of blood cells with 99mTc. Blood samples from Wistar rats were incubated with sabugueiro extract and the radiolabeling assay of blood constituents was carried out. After centrifugation, samples of plasma and blood cells were separated. Aliquots of plasma and blood cells were precipitated with trichloroacetic acid and centrifuged to isolate soluble and insoluble fractions. The radioactivity in each fraction was counted and the percentage of activity (%ATI) was determined. Incubation with sabugueiro extract altered significantly (p<0.05) the %ATI incorporated to the blood constituents. These results could be explained due the presence of chemical substances in the sabugueiro extract that present redox and/or chelating action altering the labeling of the blood constituents with 99mTc. (author)

  5. Desenvolvimento de mudas de bromélia (Neoregelia cruenta (R. Graham L. B. Smith cultivadas em diferentes substartos e adubação foliar Development of plantlets of bromeliad (Neoregelia cruenta cultivated in different substrates and leaf fertilizers

    Directory of Open Access Journals (Sweden)

    Clarissa Alves Ferreira

    2007-06-01

    extraction. This situation is also a consequence of the reduced technical information regarding its propagation and development. One of the limitations is the lack of knowledge regarding the type of substrate and adequate fertilizer for its cultivation. The objective of this study was to evaluate the development of Neoregelia cultivated in different substrates and leaf fertilizers through the variables height, leaf number and fresh and dry matter of shoots and roots. The plantlets used were produced by tissue culture. After a period of pre-acclimatization, the plantlets were transferred to a green house without nebulization. The substrates consisted of different proportions of soil, sand and carbonized rice husk and a commercial substrate composed of vermiculite. Leaf fertilizers were applied to the substrates combining urea and sucrose in intervals of fifteen days. The results showed that the interactions of the substrates and the sucrose and urea dosages had no effect on height and leaf number. The use of sucrose also showed no influence on plant development. The commercial substrate composed of vermiculite, independent of the leaf fertilizer application, showed the best results for plant height and leaf number. The use of urea presented a crescent linear effect during the evaluated period.

  6. Irradiation effect on in vitro organogenesis, callus growth and plantlet development of Gerbera jamesonii Efeito da irradiação na organogênese in vitro, crescimento de calos e desenvolvimento de plântulas de gerbera

    Directory of Open Access Journals (Sweden)

    Nor A Hasbullah

    2012-06-01

    Full Text Available The present work was carried out to study the effects of gamma irradiation on in vitro growth of explants, callus and the formation of shoots and plantlets. Irradiation is known to exhibit or inhibit the differentiation of cells and growth of plants in vitro, which helps in producing new plant varieties. Gamma irradiation is one of the physical mutagens that are widely used for mutation breeding. A gradual decline was observed in the number of shoots regenerated from irradiated petiole explants compared to control. Numbers of shoots regenerated from irradiated petiole explant cultured on Murashige & Skoog medium supplemented with 2.0 mg L-1 BAP and 0.5 mg L-1 NAA was reduced to 6.6±0.9 from 7.5±0.4 (control when explants were exposed to 20 Gray of irradiation dose. Similar observation was reported on effects of gamma irradiation on in vitro propagated plantlets. Gradual decline was observed based on plant height as the dose of gamma irradiation increased. A significant decline was observed in the fresh weight of irradiated callus compared to control. In this case, growth responses of callus were strongly influenced by the radiation dose. The fresh weight of callus was reduced to 76.4±2.2% compared to 89.7±0.5% of control when callus tissues were exposed to 20 Gy.O presente trabalho foi realizado para estudar os efeitos da radiação gama no crescimento in vitro de explantes de calos, e a formação de brotos e mudas. A irradiação é conhecida por induzir ou inibir a diferenciação de células e o crescimento das plantas in vitro, o que ajuda na produção de novas variedades vegetais. Radiação gama é um dos agentes mutagenicos que são amplamente utilizados para o melhoramento através da mutação. Um declínio gradual foi observado no número de brotos regenerados a partir de explantes de pecíolos irradiados comparado com o controle. O número de brotações regeneradas de explantes de pecíolos irradiados, cultivados em meio

  7. Adição de torta de mamona em substratos na aclimatação de mudas micropropagadas de bananeira Effect of addition of castor bean pie in substrates in acclimatization of micropropagated banana plantlets

    Directory of Open Access Journals (Sweden)

    Adriana Novais Martins

    2011-03-01

    Full Text Available A utilização de mudas micropropagadas de bananeira que oferecem qualidade genética e fitossanitária, favorecendo o desenvolvimento, instalação e uniformidade do pomar, é importante para a exploração comercial da bananicultura. O objetivo deste trabalho foi avaliar a influência da adição da torta de mamona nos substratos na aclimatação de mudas micropropagadas de bananeira da cv. Willians. O delineamento estatístico utilizado foi o inteiramente casualizado, com 10 tratamentos, sendo 2 substratos e 5 dosagens de torta de mamona (0; 6; 12; 18 e 24 g vaso-1. O substrato Vivatto Slim Plus® possibilitou o melhor desenvolvimento das plantas na aclimatação. Não são recomendadas doses superiores a 12 g planta-1 de torta de mamona misturadas ao substrato na aclimatação de mudas de bananeira.The use of micropropagated banana tree seedlings offer genetic and phytosanitary qualities, favoring the development, installation and uniformity of the yield and it is very important for the commercial exploration of banana fruits. The objective of this study was to evaluate the influence of castor oil plant pie added to the substrate in the acclimatization of micropropagated banana plantlets cv. 'Willians'. It was use the completely randomized, with 10 treatments, being 2 substrates and 5 dosages of castor oil plant pie (0; 6; 12; 18 and 24 g for each recipient. The substrate Vivatto Slim Plus® showed the best development of the plants in acclimatization processes. Dosages above 12 g of castor oil plant pie for each plant are not recommended mixed to the substrate in the acclimatization of banana tree seedlings

  8. An electrophysiological study on the effects of Pa-1G (a phospholipase A(2)) from the venom of king brown snake, Pseudechis australis, on neuromuscular function.

    Science.gov (United States)

    Fatehi, M; Rowan, E G; Harvey, A L

    2002-01-01

    The effects of Pa-1G, a phospholipase A(2) (PLA(2)) from the venom of the Australian king brown snake (Pseudechis australis) were determined on the release of acetylcholine, muscle resting membrane potential and motor nerve terminal action potential at mouse neuromuscular junction. Intracellular recording from endplate regions of mouse triangularis sterni nerve-muscle preparations revealed that Pa-1G (800 nM) significantly reduced the amplitude of endplate potentials within 10 min exposure. The quantal content of endplate potentials was decreased to 58+/-6% of control after 30 min exposure to 800 nM Pa-1G. The toxin also caused a partial depolarisation of mouse muscle fibres within 60 min exposure. Extracellular recording of action potentials at motor nerve terminals showed that Pa-1G reduced the waveforms associated with both sodium and potassium conductances. To investigate whether this was a direct or indirect effect of the toxin on these ionic currents, whole cell patch clamp experiments were performed using human neuroblastoma (SK-N-SH) cells and B82 mouse fibroblasts stably transfected with rKv1.2. Patch clamp recording experiments confirmed that potassium currents sensitive to alpha-dendrotoxin recorded from B82 cells and sodium currents in SK-N-SH cells were not affected by the toxin. Since neither facilitation of acetylcholine release at mouse neuromuscular junction nor depression of potassium currents in B82 cells has been observed, the apparent blockade of potassium currents at mouse motor nerve endings induced by the toxin is unlikely to be due to a selective block of potassium channels.

  9. [Study of the reineta protein modifications (Brama australis), put under freezing and storage to -18 degrees C and -30 degrees C].

    Science.gov (United States)

    Abugoch, Lilian; Quitral, Vilma; Larraín, M Angélica; Vinagre, Julia; Kriukov, Andrei; Chávez, Gloria

    2006-12-01

    The objective of the present work was to study functional and thermal properties of reineta (Brama australis) frozen meat, analysed by water retention capacity (WRC), gel forming capacity (GFC), texture, emulsifying capacity and differential scanning calorimetry (DSC). For this study, reineta fillets were obtained and extracted by the same conditions, and cutted, packaged, frozen and stored at -18 degrees C and -30 degrees C for 7 months. The results obtained, showed that there were no signifficant differences in the responses to thermal treatment for all the specimens. For samples frozen at -18 degrees C and -30 degrees C, the protein contents were 23.5 + 0.0 and 25.4 + 1.0%, respectively. The WRC values were 0.45 + 0.1 and 1.59 +/- 0.0 g water/g protein, respectively. The gel forming capacity was only present in the fresh samples, whereas the frozen stored ones only form protein aggregates. The emulsifying capacity was between 960 and 1400 g oil / g protein, and the storage time increased this value. The miosin denaturation temperature (Td) and denaturation enthalpy (?H), obtained by DSC, fluctuated between 39.2 +/- 0.5 to 44.8 +/- 0.8 degrees C and 1.12 +/- 0.3 to 0.52 +/- 0.2 J/g, respectively. The actina values were between 71.0 +/- 0.6 to 75.3 +/- 0.5 degrees C and between 0.5 +/- 0.1 to 0.7 +/- 0.1 J/g. Cooperativity decreased as the storage time increased. This is showing a certain degree of protein displacement. The values found by thermal analyses showed a direct relationship with the functional properties, both decreasing with storage time.

  10. Long-term responses of Burrunan dolphins (Tursiops australis to swim-with dolphin tourism in Port Phillip Bay, Victoria, Australia: A population at risk

    Directory of Open Access Journals (Sweden)

    Nicole E. Filby

    2014-12-01

    Full Text Available This study investigated Burrunan dolphin responses to dolphin-swim tour vessels across two time periods: 1998–2000 and 2011–2013. A total of 211 dolphin sightings were documented across 306 surveys. Sighting success rate and mean encounter time with dolphins decreased significantly by 12.8% and 8.2 min, respectively, between periods. Approaches that did not contravene regulations elicited highest approach responses by dolphins towards tour vessels, whereas dolphins’ responded to illegal approaches most frequently with avoidance. Small groups responded to tour vessels with avoidance significantly more than large groups. Initial dolphin behaviour had a strong effect on dolphin’s responses to tour vessels, with resting groups the most likely to exhibit avoidance. Calves were significantly more likely to be present during swims in 2011–2013. Dolphin’s responses to tour vessels changed across time, with effect responses (avoidance and approach increasing significantly as dolphins gained cumulative experience. These dolphins are forced to expend a greater level of time and energy avoiding or approaching boats, shifting from a non-effect response to an effect response. Consequences of this include possible decrease in biological fitness by detracting from core biological activities such as foraging and resting. Combined with a decrease in sighting success between periods, the results imply that this population of dolphins, which is endemic to Australia and listed as threatened under the Victorian Flora and Fauna Guarantee Act 1988, may not be well suited to the dolphin-swim industry. The management implications of these results warrant a shift from passive to active management in Port Phillip Bay. The importance of long-term research is highlighted, given behavioural responses detected herein would be undetected in short-term studies. Keywords: Burrunan dolphin (Tursiops australis, Avoidance, Behaviour, Compliance, Management

  11. Increased Wounding of Southern Right Whale (Eubalaena australis Calves by Kelp Gulls (Larus dominicanus at Península Valdés, Argentina.

    Directory of Open Access Journals (Sweden)

    Carina F Marón

    Full Text Available At least 626 southern right whale (Eubalaena australis calves died at the Península Valdés calving ground, Argentina, between 2003 and 2014. Intense gull harassment may have contributed to these deaths. In the 1970s, Kelp Gulls (Larus dominicanus began feeding on skin and blubber pecked from the backs of living right whales at Valdés. The frequency of gull attacks has increased dramatically over the last three decades and mother-calf pairs are the primary targets. Pairs attacked by gulls spend less time nursing, resting and playing than pairs not under attack. In successive attacks, gulls open new lesions on the whales' backs or enlarge preexisting ones. Increased wounding could potentially lead to dehydration, impaired thermoregulation, and energy loss to wound healing. The presence, number and total area of gull-inflicted lesions were assessed using aerial survey photographs of living mother-calf pairs in 1974-2011 (n = 2680 and stranding photographs of dead calves (n = 192 in 2003-2011. The percentage of living mothers and calves with gull lesions increased from an average of 2% in the 1970s to 99% in the 2000s. In the 1980s and 1990s, mothers and calves had roughly equal numbers of lesions (one to five, but by the 2000s, calves had more lesions (nine or more covering a greater area of their backs compared to their mothers. Living mother-calf pairs and dead calves in Golfo Nuevo had more lesions than those in Golfo San José in the 2000s. The number and area of lesions increased with calf age during the calving season. Intensified Kelp Gull harassment at Península Valdés could be compromising calf health and thereby contributing to the high average rate of calf mortality observed in recent years, but it cannot explain the large year-to-year variance in calf deaths since 2000.

  12. Globalisation and the Australian Aborigines: gain or strain? La Mondialisation : une chance pour les Aborigènes d’Australie ?

    Directory of Open Access Journals (Sweden)

    Ludivine Royer

    2009-10-01

    Full Text Available La mondialisation est un phénomène très complexe, qui implique à la fois l’émergence de l’économie de marché, l’accélération du commerce international, l’ouverture des frontières, la libre circulation des personnes et des biens, le développement extraordinaire des transports et de la technologie de l’information, la montée en puissance des télécommunications et des médias à l’échelle planétaire, l’expansion des cultures occidentales et le déclin des États-nations au profit d’institutions internationales en plein essor. Un nouvel ordre mondial est né, et comme la plupart des autres pays, l’Australie a connu de profonds changements depuis la fin des années 1980. Cet article se propose de montrer l’impact direct et indirect de la mondialisation économique, culturelle, technologique et politique sur le peuple aborigène. Bien entendu, il ne s’agit pas de présenter de manière exhaustive les conséquences multiples et complexes de la mondialisation sur les droits, cultures, et sociétés aborigènes, leur développement économique et leur statut politique. Il s’attelle en revanche à mettre en évidence les logiques politiques et socio-culturelles que la mondialisation a favorisées dans les affaires aborigènes, de l’assimilation à l’auto-détermination.

  13. Marine Bacteria from Danish Coastal Waters Show Antifouling Activity against the Marine Fouling Bacterium Pseudoalteromonas sp. Strain S91 and Zoospores of the Green Alga Ulva australis Independent of Bacteriocidal Activity

    DEFF Research Database (Denmark)

    Bernbom, Nete; Ng, Yoke Yin; Kjelleberg, Staffan

    2011-01-01

    , representing the major taxonomic groups, different seasons, and isolation strategies, were tested for antiadhesive effect against the marine biofilm-forming bacterium Pseudoalteromonas sp. strain S91 and zoospores of the green alga Ulva australis. The antiadhesive effects were assessed by quantifying...... the number of strain S91 or Ulva spores attaching to a preformed biofilm of each of the 22 strains. The strongest antifouling activity was found in Pseudoalteromonas strains. Biofilms of Pseudoalteromonas piscicida, Pseudoalteromonas tunicata, and Pseudoalteromonas ulvae prevented Pseudoalteromonas S91 from...

  14. Diurnal dynamics of oxygen and carbon dioxide concentrations in shoots and rhizomes of a perennial in a constructed wetland indicate down-regulation of below ground oxygen consumption

    Czech Academy of Sciences Publication Activity Database

    Fausser, A. C.; Dušek, Jiří; Čížková, Hana; Kazda, M.

    2016-01-01

    Roč. 8, JUL (2016), č. článku plw025. ISSN 2041-2851 R&D Projects: GA MŠk(CZ) ED1.1.00/02.0073; GA MŠk(CZ) LM2010007 Institutional support: RVO:86652079 Keywords : typha-latifolia l * internal gas-transport * phragmites-australis * convective throughflow * pressurized ventilation * angustifolia l * ex steud * roots * flow * respiration * Aeration * constructed wetland * in-situ field study * internal carbon dioxide * internal oxygen dynamics * Phragmites australis Subject RIV: EF - Botanics OBOR OECD: Plant sciences, botany Impact factor: 2.238, year: 2016

  15. Substratos alternativos ao xaxim e adubação de plantas de orquídea na fase de aclimatização Alternative substrates to xaxim and fertilization on orchids plantlets acclimatization phase

    Directory of Open Access Journals (Sweden)

    Aparecida Gomes de Araujo

    2007-04-01

    Full Text Available Objetivou-se estudar substratos e adubação em plantas de orquídea da espécie C. loddgesii "Alba" x C. loddgesii "Atibaia". Plantas oriundas de cultivo in vitro foram transplantadas para bandejas coletivas contendo pó de xaxim e aclimatizadas em casa de vegetação. Após seis meses, quando as plantas tinham de 3,0 a 5,0cm de comprimento, foram transferidas para bandejas de plástico com 24 células de 150cm³ cada contendo os substratos brita no 0, casca de arroz carbonizada (CAC, xaxim desfibrado e fibra de piaçava e submetidas a adubações semanais com Biofert Plus® (5,0mL L-1, Dyna-Grow® (2,5mL L-1 e formulação elaborada. Após 12 meses, verificou-se que os melhores substratos para plantas da orquídea C. loddgesii "Alba" x C. loddgesii "Atibaia" são casca de arroz carbonizada e fibra de piaçava, e melhores respostas à adubação são obtidas com o adubo foliar Biofert Plus®.This paper was aimed at studing different substrates and fertilization for the development of C. loddgesii 'Alba' x C. loddgesii 'Atibaia' orchid species. Seedlings from in vitro culture were transplanted to plastic trays containing xaxim powder. After 6-months of acclimatization inside a greenhouse, plantles measuring beetwen 3.0 to 5.0cm length were transferred to 24 cell trays with a volume of 150cm³. Four substrates were tested (broken stone number 0, carbonized rice hulls, defibered xaxim and piassava fiber with tree different fertilizations (Biofert Plus®, Dyna Grow® and a specific formulation developed for this work. Pure water was tested as control. After 12-months of growth the best treatments for the acclimatization of in vitro produced C. loddgesii 'Alba' x C. loddgesii 'Atibaia' orchid plantlets were carbonized rice hulls or piassava fiber substrates combined with leaf fertilizer Biofert Plus®.

  16. Caracterização farmacobotânica das espécies de Sambucus (Caprifoliaceae utilizadas como medicinais no Brasil: Parte II. Sambucus australis Cham. & Schltdl.

    Directory of Open Access Journals (Sweden)

    Eliana Nunes

    Full Text Available Sambucus australis Cham. & Schltdl. (sabugueiro-do-rio-grande, Caprifoliaceae é uma planta medicinal descrita apenas na primeira edição da Farmacopéia Brasileira. A espécie é nativa no Brasil, e suas flores são utilizadas na medicina popular, na forma de infusão ou decocção, como diuréticas, antipiréticas, antiinflamatórias, laxativo leve e no tratamento de doenças do aparelho respiratório. Visando elaborar uma monografia farmacopéica atualizada e comparativa com a de Sambucus nigra L., de origem européia, foram estabelecidos os caracteres botânicos macro e microscópicos, através da metodologia clássica utilizada em morfoanatomia vegetal. São características macroscópicas: flores morfologicamente monoclinas; corola de sete a dez milímetros de diâmetro; pétalas com cinco, raro quatro nervuras paralelas; estames curtos e longos; gineceu em regra com cinco lóculos. São características microscópicas: cutícula espessa e estriada; ausência de idioblastos de areia cristalina de oxalato de cálcio; estômatos anomocíticos; brácteas anfiestomáticas; sépalas hipoestomáticas; pétalas anfi-hipoestomáticas; células epidérmicas da face abaxial de sépalas e pétalas retilíneas a sinuosas; células epidérmicas do filete alongadas e de paredes retilíneas; tricomas tectores e glandulares de diferentes tipos; brácteas, sépalas e pétalas com mesofilo homogêneo; sistema vascular representado por feixes colaterais ou agrupamentos de elementos xilemáticos; presença de gotas lipídicas em todas as peças.

  17. Somatic embryogenesis and plantlet regeneration from protoplast ...

    African Journals Online (AJOL)

    After 4 weeks of culture, microcalli appeared on the surface of the Ca-alginate beads. Growth of microcalli in the medium with nurse cells (33.3%) was much better than those in the medium without nurse cells (6.5%). Transferring beads onto MS medium supplemented with 0.1 mg/l BA increased the growth of embryogenic ...

  18. Genetic fidelity in micropropagated plantlets of Ochreinauclea ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-07-06

    Jul 6, 2009 ... show the genetic similarity among the 22 plants (21 micropropagated plants and 1 donor mother plant). In the present study, ..... Determination of genetic stability in long-term micropropagated shoots of Pinus thunbergii Parl. using. RAPD markers. Plant Cell Rep. 18: 193-197. Guo WL, Gong L, Ding ZF, ...

  19. « Murayana va à Garma cette année ! » : cérémonies publiques et rituels contemporains du nord-est de la Terre d’Arnhem (Australie)

    OpenAIRE

    De Largy Healy, Jessica

    2011-01-01

    Partant de l’idée que les cérémonies yolngu du nord-est de la Terre d'Arnhem (Australie) relèvent de négociations dynamiques entre des individus, des clans et des ancêtres, cet article examine comment une série d'échanges rituels furent articulés dans le cadre d’un festival culturel ouvert aux touristes. Je montre comment un projet de patrimonialisation numérique insuffla un élan de créativité rituelle dans plusieurs localités de la région et aboutit à une véritable campagne de représentation...

  20. Genotypic differences in proembryoid development and green plantlets regeneration through androgenesis in barley varieties Diferenças genotípicas no desenvolvimento de pró-embrióides e regeneração de plântulas verdes via androgênese em genótipo de cevada

    Directory of Open Access Journals (Sweden)

    Paula Wiethölter

    2008-02-01

    Full Text Available The development of in vitro haploid plants followed by spontaneous or induced genome duplication allows to achieve, in one generation, the recovery of total homozygosis. The efficiency of the haplodiploidization process through in vitro anther culture of barley is variable among genotypes. This study was aimed at determining the androgenetic response of nine barley genotypes from the breeding program of Embrapa Trigo, analysing proembryoid development and green plantlets regeneration in anthers cultivated in vitro. Cultivar 'BR2' presented the highest average of proembryoids (104/anther and 'MN698' presented the highest average of green plantlets (0,41/anther. There was a significant variation among the average values of barley genotypes for embryo formation and green plantlets regeneration, making possible the selection to combine androgenetic capacity and good agronomic traits.A obtenção, na cevada, de plantas haplóides in vitro e a posterior duplicação natural ou artificial do genoma permitem alcançar a homozigose completa, em uma geração. A eficiência da haplodiploidização pela cultura de anteras é variável entre os genótipos. Foi avaliada a resposta androgenética através da formação de pró-embrióides e da regeneração de plântulas verdes em nove cultivares do programa de melhoramento de cevada da Embrapa Trigo, em anteras cultivadas in vitro. A cultivar "BR2" apresentou maior média de pró-embrióides (104/antera, enquanto "MN698" mostrou a maior média de plântulas verdes (0,41/antera. Houve variação significativa entre os valores médios dos genótipos em relação à formação de pró-embrióides e à regeneração de plântulas verdes, indicando a possibilidade de seleção para combinar a capacidade androgenética com boas características agronômicas.

  1. ALL THAT "PHRAG": BRINGING ENGINEERING, WETLAND ECOLOGY, ENVIRONMENTAL SCIENCE, AND LANDSCAPE ECOLOGY TO BEAR ON THE QUESTION OF COMMON REED IN GREAT LAKES COASTAL WETLANDS

    Science.gov (United States)

    Coastal wetlands are among the most fragmented and disturbed ecosystems and the Great Lakes are no exception. One possible result is the observed increase in the presence and dominance of invasive and other opportunistic plant species, such as the common reed (Phragmites australi...

  2. Nutrient Cycling and Retention Along a Littoral Gradient in a Dutch Shallow Lake in Relation to Water Level Regime

    NARCIS (Netherlands)

    Sollie, S.; Verhoeven, J.T.A.

    Littoral zones are characterized by gradients in depth and vegetation biomass, influencing nutrient retention capacity. A field experiment was conducted in a Phragmites australis dominated littoral zone to investigate nutrient retention and its effect on surface water quality. Measurements were done

  3. Fate of xenobiotic compounds and plants activity in reed bed sludge treatment

    DEFF Research Database (Denmark)

    Chen, Xijuan; Pauli, Udo; Rehfus, Stefan

    different plants: bulrush (Typha), reed (Phragmites australis) and reed canary grass (Phalaris arundinacea) were planted into 12 containers with a size of 1m Х 1m X 1m which were builded with 20cm gravel and 50cm sludge to study the plants activity in sludge degradation process, 4 containers were left...

  4. Enige diatomeeënsoorten, nieuw voor de Nederlandse flora

    NARCIS (Netherlands)

    Dam, van Herman

    1974-01-01

    During an investigation of the epiphyton, growing on the culms of Phragmites australis, Typha latifolia, and Glyceria maxima, in the nature-reserve “Het Naardermeer”, ten species of diatoms were discovered which are new for the Dutch flora. These are enumerated together with some notes on their

  5. Seasonal and spatial variations in heterotrophic nanoflagellate and bacteria abundances in sediments of a freshwater littoral zone

    NARCIS (Netherlands)

    Starink, Mathieu; Bär-Gilissen, M.J.; Cappenberg, T.E.

    1996-01-01

    We studied seasonal variation in heterotrophic nanoflagellates (HNAN) and bacterial, densities at different depths in the sediment of two freshwater littoral stations. Station 1 was in a reed bed of Phragmites australis; station 2 was outside the reed zone in open water. Benthic HNAN abundances

  6. Mississippi River, Baton Rouge to the Gulf, Louisiana, Project. Supplement II.

    Science.gov (United States)

    1984-04-01

    Hlydrocotyl Persimmon Diospyros virginiana Picke relweed Pontederia cordata Rattlebox Daubentonia drummundii Rose au Phragmites australis Saltgrass Distichlis...menhaden, genus Brevoortia, with respect to salinity, in the upper drainage basin of Barataria Bay, Louisiana. M.S. Thesis. Louisiana State University

  7. Ecology, fish and fishery of Lake Liambezi, a recently refilled ...

    African Journals Online (AJOL)

    Lake Liambezi (300 km2) refilled in 2009 after a prolonged 22-year dry period. Its aquatic macrophyte populations, fish fauna and fishery shortly after refilling are described. The emergent aquatic macrophyte Phragmites australis formed dense stands covering large parts of the lake, while extensive beds of submerged ...

  8. Comparison of metabolisable energy values of different foodstuffs determined in ostriches and poultry

    DEFF Research Database (Denmark)

    Cilliers, S C; Sales, J; Hayes, J P

    1999-01-01

    Apparent (AMEn) and true (TMEn) metabolisable energy values, corrected for nitrogen retention, of wheat bran, saltbush (Atriplex nummularia), common reed (Phragmites australis), lupins, soyabean oil cake meal (SBOCM), sunflower oil cake meal (SFOCM) and fishmeal were compared in 7 successive trials...

  9. Effects of two common macrophytes on methane dynamics in freshwater sediments

    NARCIS (Netherlands)

    Van der Nat, F.J.; Middelburg, J.J.

    1998-01-01

    The methane cycle in constructed wetlands without plants and with Phragmites australis (reed) and Scirpus lacustris (bulrush) was investigated. Variations in CH4 production largely determined variations in CH4 emission among the systems: rather than variations in CH4 storage and oxidation. Twofold

  10. Factors influencing CO2 and CH4 emissions from coastal wetlands in the Liaohe Delta, Northeast China

    DEFF Research Database (Denmark)

    Olsson, L.; Ye, S.; Wei, M.

    2015-01-01

    temperature and vegetation on CH4 emissions and ecosystem respiration (Reco) from five coastal wetlands in the Liaohe Delta, northeast China: two Phragmites australis (common reed) wetlands, two Suaeda salsa (sea blite) marshes and a rice (Oryza sativa) paddy. Throughout the growing season, the Suaeda...

  11. The impacts of ditch cuttings on weed pressure and crop yield in maize

    NARCIS (Netherlands)

    Huijser, M.P.; Meerburg, B.G.; Holshof, G.

    2004-01-01

    The Flevoland Polder in The Netherlands is characterised by large agricultural fields separated by ditches. The vegetation in the ditches is typically cut twice a year to ensure sufficient drainage. Removal of the cuttings, primarily reed (Phragmites australis), results in a substantial increase in

  12. A Regional Guidebook for Applying the Hydrogeomorphic Approach to Assessing the Functions of Tidal Fringe Wetlands Along the Mississippi and Alabama Gulf Coast

    Science.gov (United States)

    2007-04-01

    philoxeroides Alligatorweed Alabama Class C noxious weed Imperata cylindrica Cogongrass Alabama Class A noxious weed; Mississippi noxious weed Ipomoea...Invasive Species Alternanthera philoxeroides Phragmites australis Cuscuta spp. Imperata cylindrica...weed Cuscuta spp. Dodder Alabama Class A noxious weed Imperata cylindrica Cogongrass Alabama Class A noxious weed; MS noxious weed Ipomoea

  13. Investigation into the kinetics of constructed wetland degradation ...

    African Journals Online (AJOL)

    -scale, horizontal subsurface-flow constructed wetland (6.0 m × 1.0 m × 0.5 m) in Leipzig, Germany. The bed contained glacial gravel (4–8 mm) planted with Phragmites australis. Construction was completed in October 2013 and experiments ...

  14. AIRBORNE HYPERSPECTRAL IDENTIFICATION OF INVASIVE AND OPPORTUNISTIC WETLANDS PLANT SPECIES

    Science.gov (United States)

    Coastal wetlands are among the most fragmented and disturbed ecosystems and the Great Lakes are no exception. One possible result is the observed increase in the presence and dominance of invasive and other opportunistic plant species, such as the common reed (Phragmites australi...

  15. Adubação foliar com macro e micronutrientes no crescimento de mudas micropropagadas do abacaxizeiro cv. Gold [Ananas comosus (L. Merrill] em diferentes recipientes Foliar fertilization with macro and micronutrients in the growth of plantlets micropropagated of pineapple cv. Gold [Ananas comosus (L. Merrill] in different containers

    Directory of Open Access Journals (Sweden)

    Izaias dos Santos Bregonci

    2008-06-01

    Full Text Available Objetivou-se com este trabalho avaliar o efeito da adubação foliar com macro e micronutrientes no crescimento das mudas micropropagadas do abacaxizeiro cv. Gold [Ananas comosus (L. Merrill], em diferentes recipientes. O experimento foi em esquema fatorial 8x3, adubação foliar em 8 níveis e recipientes em 3 níveis, através de um delineamento inteiramente casualizado com 5 repetições. As mudas foram padronizadas com altura média de 7,12 cm. As adubações foliares foram feitas com uréia, cloreto de potássio, ácido bórico, um formulado comercial com macro e micronutrientes e testemunha (pulverização com água e os recipientes: bandeja de isopor com 200 células; tubete pequeno de 115 cm³; e tubete grande com 300 cm³. O substrato utilizado foi o plantmax hortaliças®. Avaliaram-se as características área foliar, altura de planta e massa seca da parte aérea e da raiz, aos 140 dias do transplantio. Os adubos foliares proporcionaram maior crescimento em área foliar, altura e massa seca da parte aérea às mudas do abacaxizeiro, embora com resultados diferentes. Os adubos foliares não aumentaram a massa seca do sistema radicular. A bandeja de isopor apresentou as menores médias, com todos os adubos foliares para área foliar, altura e massa seca da parte aérea das mudas do abacaxizeiro. O tubete pequeno e o tubete grande apresentaram resultados semelhantes com a maioria dos adubos foliares utilizados.The objective of this work was to evaluate the effect of the foliar fertilization with macro and micronutrients on the growth of the plantlets micropropagated of pineapple cv. Gold [Ananas comosus (L. Merrill] in different containers. The experiment was mounted in factorial arrangement 8x3, with foliar fertilization in 8 levels and containers in 3 levels, through a completely randomized design with five repetitions. The plantlets were standardized with average height of 7,12 cm. The foliar fertilization was used in the urea

  16. Anomalous colour in Neotropical mammals: a review with new records for Didelphis sp. (Didelphidae, Didelphimorphia and Arctocephalus australis (Otariidae, Carnivora Coloração anômala em mamíferos Neotropicais: uma revisão com novos registros para Didelphis sp. (Didelphidae, Didelphimorphia e Arctocephalus australis (Otariidae, Carnivora

    Directory of Open Access Journals (Sweden)

    MSL. Abreu

    2013-02-01

    Full Text Available Anomalous colourations occur in many tropical vertebrates. However, they are considered rare in wild populations, with very few records for the majority of animal taxa. We report two new cases of anomalous colouration in mammals. Additionally, we compiled all published cases about anomalous pigmentation registered in Neotropical mammals, throughout a comprehensive review of peer reviewed articles between 1950 and 2010. Every record was classified as albinism, leucism, piebaldism or eventually as undetermined pigmentation. As results, we report the new record of a leucistic specimen of opossum (Didelphis sp. in southern Brazil, as well as a specimen of South American fur seal (Arctocephalus australis with piebaldism in Uruguay. We also found 31 scientific articles resulting in 23 records of albinism, 12 of leucism, 71 of piebaldism and 92 records classified as undetermined pigmentation. Anomalous colouration is apparently rare in small terrestrial mammals, but it is much more common in cetaceans and michrochiropterans. Out of these 198 records, 149 occurred in cetaceans and 30 in bats. The results related to cetaceans suggest that males and females with anomolous pigmentation are reproductively successful and as a consequence their frequencies are becoming higher in natural populations. In bats, this result can be related to the fact these animals orient themselves primarily through echolocation, and their refuges provide protection against light and predation. It is possible that anomalous colouration occurs more frequently in other Neotropical mammal orders, which were not formally reported. Therefore, we encourage researchers to publish these events in order to better understand this phenomenon that has a significant influence on animal survival.Colorações anômalas ocorrem em muitos vertebrados tropicais. Entretanto, estas são consideradas raras em populações selvagens, havendo poucos registros para a maioria dos táxons. Reportam

  17. Effects of interspecific competition on the growth of macrophytes and nutrient removal in constructed wetlands: A comparative assessment of free water surface and horizontal subsurface flow systems.

    Science.gov (United States)

    Zheng, Yucong; Wang, Xiaochang; Dzakpasu, Mawuli; Zhao, Yaqian; Ngo, Huu Hao; Guo, Wenshan; Ge, Yuan; Xiong, Jiaqing

    2016-05-01

    The outcome of competition between adjoining interspecific colonies of Phragmites and Typha in two large field pilot-scale free water surface (FWS) and subsurface flow (SSF) CWs is evaluated. According to findings, the effect of interspecific competition was notable for Phragmites australis, whereby it showed the highest growth performance in both FWS and SSF wetland. In a mixed-culture, P. australis demonstrates superiority in terms of competitive interactions for space between plants. Furthermore, the interspecific competition among planted species seemed to cause different ecological responses of plant species in the two CWs. For example, while relatively high density and shoot height determined the high aboveground dry weight of P. australis in the FWS wetland, this association was not evident in the SSF. Additionally, while plants nutrients uptake accounts for a higher proportion of the nitrogen removal in FWS, that in the SSF accounts for a higher proportion of the phosphorous removal. Copyright © 2016 Elsevier Ltd. All rights reserved.

  18. A novel association between Rhodnius neglectus and the Livistona australis palm tree in an urban center foreshadowing the risk of Chagas disease transmission by vectorial invasions in Monte Alto City, São Paulo, Brazil.

    Science.gov (United States)

    Carvalho, Danila B; Almeida, Carlos E; Rocha, Cláudia S; Gardim, Sueli; Mendonça, Vagner J; Ribeiro, Aline R; Alves, Zulimar C P V T; Ruellas, Kellem T; Vedoveli, Alan; da Rosa, João A

    2014-02-01

    After several public notifications of domiciliary invasions, palm trees were investigated in downtown Monte Alto City, São Paulo State, Brazil, in proximity to the city hall building, the main church, condominiums and marketing establishments. One hundred seventy four palm trees of 10 species were investigated, in which 72 specimens of Rhodnius neglectus, a potential Chagas disease vector, were captured via manual methods. All insects were collected from dead leaves, organic debris and bird nests in the only three Livistona australis palm trees in the central park square. This was the first record of R. neglectus colonizing this palm species. Although no Trypanosoma cruzi was found by abdominal compression followed by light microscopy, the poor nutritional status of the bugs hampered the examination of gut contents for parasite detection. Furthermore, the central crowns of the trees, which shelter bats (Chiroptera: Mammalia), could not be carefully searched for insects due to difficult access. This new finding highlights the sudden alteration in insect behavior, probably as a result of man's interference. This report aims to warn those involved in the health system about this new threat, justifying detailed research of the area to evaluate the magnitude of this emerging public health issue. Copyright © 2013 Elsevier B.V. All rights reserved.

  19. Effects of species, sex, length, and locality on the mercury content of school shark Galeorhinus australis (Macleay) and gummy shark Mustelus antarcticus Guenther from south-eastern Australian waters

    Energy Technology Data Exchange (ETDEWEB)

    Walker, T.I.

    1976-12-01

    The mercury levels detected in the muscle tissues of sharks ranged from 0.01 to 2.7 ppM wet weight for school shark Galeorhinus australis (Macleay) and from 0.07 to 3.0 ppM for gummy shark Mustelus antarcticus Guenther. Estimates of the mean mercury levels for the 1971 Victorian landed commercial shark catch were found to be 0.90 ppM for the school shark and 0.37 ppM for the gummy shark. The analyses for total mercury determinations were carried out by five independent laboratories. Preliminary analyses carried out by one indicated that most of the mercury in school sharks and about two-thirds of the mercury in gummy sharks was present as methylmercury. The mercury concentrations varied exponentially with shark length. School sharks had statistically significant higher mercury levels than gummy sharks of the same length and for both the medium-sized and large individuals of each species males had significantly higher levels than females. Levels in male gummy sharks were found to be affected by locality.

  20. Structures of Bordered Pits Potentially Contributing to Isolation of a Refilled Vessel from Negative Xylem Pressure in Stems of Morus australis Poir.: Testing of the Pit Membrane Osmosis and Pit Valve Hypotheses.

    Science.gov (United States)

    Ooeda, Hiroki; Terashima, Ichiro; Taneda, Haruhiko

    2017-02-01

    Two hypotheses have been proposed to explain the mechanism preventing the refilling vessel water from being drained to the neighboring functional vessels under negative pressure. The pit membrane osmosis hypothesis proposes that the xylem parenchyma cells release polysaccharides that are impermeable to the intervessel pit membranes into the refilling vessel; this osmotically counteracts the negative pressure, thereby allowing the vessel to refill. The pit valve hypothesis proposes that gas trapped within intervessel bordered pits isolates the refilling vessel water from the surrounding functional vessels. Here, using the single-vessel method, we assessed these hypotheses in shoots of mulberry (Morus australis Poir.). First, we confirmed the occurrence of xylem refilling under negative pressure in the potted mulberry saplings. To examine the pit membrane osmosis hypothesis, we estimated the semi-permeability of pit membranes for molecules of various sizes and found that the pit membranes were not semi-permeable to polyethylene glycol of molecular mass osmosis mechanism in mulberry would be unrealistically large. © The Author 2016. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  1. Formação do plexo braquial e sistematização dos territórios nervosos em membros torácicos de lobos-marinhos Arctocephalus australis

    Directory of Open Access Journals (Sweden)

    Daniel Alexandre Stüpp de Souza

    2010-04-01

    Full Text Available For the study concerning the brachial plexus (BP and the nervous territories of the thoracic limb of South American Fur Seal (Arctocephalus australis, two animals had been used. Their skin was removed and the musculature of the thoracic limb and pectoral region was identified. After that compresses of glacial acetic acid solution of 3% had been applied in the musculature, in order to facilitate the carried through dissection. In analyzed specimens it was observed the emergency of BP from the sixth cervical nerve until the first thoracic nerve. From these four roots trunks of nerves of the same number are formed, whose ventral branches will constitute its arrangement and territorial distribution. From these four trunks emerge the 12 component nerves of the BP, some of them come from only one segment (supra scapular, cranial pectoral, lateral thoracic, thoraco-dorsal and long thoracic, while others come from more than a segment (sub scapular, muscle-cutaneous, axillary, median, caudal pectoral, ulnar and radial. In this direction,this observed constancy in the innervation of the musculature, joints and bones of the thoracic limb, led us to infer that a definite pattern in the delimitation of the nervous territories clearly exists.

  2. The effects of two phospholipase A2 inhibitors on the neuromuscular blocking activities of homologous phospholipases A2 from the venom of Pseudechis australis, the Australian king brown snake.

    Science.gov (United States)

    Fatehi, M; Rowan, E G; Harvey, A L

    1995-12-01

    Previous studies have shown that homologous phospholipases A2 (PLA2) (Pa-3, Pa-9C, Pa-10F and Pa-11) from the venom of the Australian king brown snake, Pseudechis australis, significantly reduce the resting membrane potentials and quantal contents of endplate potentials recorded from endplate regions of mouse triangularis sterni nerve-muscle preparations. It is not clear whether PLA2 activity is essential for their neuromuscular activities. Therefore, pharmacological studies were carried out to determine whether neuromuscular activity of the toxins changed after treatment with the phospholipase A2 inhibitors 7,7-dimethyl-eicosadienoic acid (DEDA) and manoalide. After incubation of the toxins with manoalide (120 nM), or DEDA (50 microM), no PLA2 activity against 1-stearoyl 2-[3H]arachidonoylglycerophosphocholine was detected. After incubation with manoalide and/or DEDA, the toxins did not depolarize muscle fibre membranes up to 60 min after administration. However, manoalide and DEDA had different influences on the inhibitory effect of these toxic enzymes on acetylcholine release from nerve terminals. Manoalide abolished the inhibitory effect of the toxins on evoked release of acetylcholine. In contrast, DEDA was not able to prevent the reduction of quantal content of endplate potentials induced by the toxins. This study provides evidence that the depolarizing action and the inhibitory effect on release of acetylcholine exerted by these toxic PLA2 from king brown snake are independent phenomena. The evidence for this conclusion was that inhibition of enzymatic activity with an arachidonic acid analogue (DEDA) abolished the depolarizing effect of the toxins but not the effects on the quantal release of acetylcholine from mouse motor nerve terminals. The data suggest that the depolarizing effect of these toxins is probably due to the enzymatic activity. Since manoalide interacts with lysine residues of PLA2 polypeptides, and, as shown here, manoalide prevented

  3. Marine Bacteria from Danish Coastal Waters Show Antifouling Activity against the Marine Fouling Bacterium Pseudoalteromonas sp. Strain S91 and Zoospores of the Green Alga Ulva australis Independent of Bacteriocidal Activity▿†

    Science.gov (United States)

    Bernbom, Nete; Ng, Yoke Yin; Kjelleberg, Staffan; Harder, Tilmann; Gram, Lone

    2011-01-01

    The aims of this study were to determine if marine bacteria from Danish coastal waters produce antifouling compounds and if antifouling bacteria could be ascribed to specific niches or seasons. We further assess if antibacterial effect is a good proxy for antifouling activity. We isolated 110 bacteria with anti-Vibrio activity from different sample types and locations during a 1-year sampling from Danish coastal waters. The strains were identified as Pseudoalteromonas, Phaeobacter, and Vibrionaceae based on phenotypic tests and partial 16S rRNA gene sequence similarity. The numbers of bioactive bacteria were significantly higher in warmer than in colder months. While some species were isolated at all sampling locations, others were niche specific. We repeatedly isolated Phaeobacter gallaeciensis at surfaces from one site and Pseudoalteromonas tunicata at two others. Twenty-two strains, representing the major taxonomic groups, different seasons, and isolation strategies, were tested for antiadhesive effect against the marine biofilm-forming bacterium Pseudoalteromonas sp. strain S91 and zoospores of the green alga Ulva australis. The antiadhesive effects were assessed by quantifying the number of strain S91 or Ulva spores attaching to a preformed biofilm of each of the 22 strains. The strongest antifouling activity was found in Pseudoalteromonas strains. Biofilms of Pseudoalteromonas piscicida, Pseudoalteromonas tunicata, and Pseudoalteromonas ulvae prevented Pseudoalteromonas S91 from attaching to steel surfaces. P. piscicida killed S91 bacteria in the suspension cultures, whereas P. tunicata and P. ulvae did not; however, they did prevent adhesion by nonbactericidal mechanism(s). Seven Pseudoalteromonas species, including P. piscicida and P. tunicata, reduced the number of settling Ulva zoospores to less than 10% of the number settling on control surfaces. The antifouling alpP gene was detected only in P. tunicata strains (with purple and yellow pigmentation), so

  4. Reducing uncertainty and bias in acoustic biomass estimations of southern blue whiting (Micromesistius australis in the southeastern Pacific: transducer motion effects upon acoustic attenuation Reduciendo el sesgo e incertidumbre de las estimaciones hidroacústicas de biomasa de merluza de tres aletas (Micromesistius australis en el Pacífico suroriental: efectos del movimiento del transductor sobre la atenuación acústica

    Directory of Open Access Journals (Sweden)

    Alvaro Saavedra

    2012-09-01

    Full Text Available We evaluated the loss of sensitivity due to the motion experienced by a hull-mounted transducer and its effects upon the estimated biomass of southern blue whiting (Micromesistius australis in an acoustic survey conducted in the southeastern Pacific, off the Chilean coast, during the austral winter of 2009. Vessel motion data (pitch and roll were registered in situ using a digital clinometer and used to correct the nautical area scattering coefficients (NASC in elementary sampling units of 926 m distance by 10 m depth. These NASC correction factors (NASCcp were calculated using Dunford's algorithm for circular transducers. We found high variability in NASCcp, which averaged 12%, and ranged between 0 and 50%. NASCcp variability was explained significantly by the mean depth of the integrated stratum (33%, the weather condition, as measured by Beaufort's scale (5%, and the vessel course relative to wind direction (2%. The empirical model we used to explain NASC Cp variability may be suitable for correcting bias due to transducer motion in other, past and future, fisheries acoustic surveys targeting mid-water species under rough weather conditions.Se evaluó la pérdida de sensibilidad producida por el movimiento de un transductor montado en el casco y sus efectos sobre la abundancia y biomasa estimada de merluza de tres aletas (Micromesistius australis en un estudio acústico realizado en el Pacífico suroriental, frente a la costa de Chile, durante el invierno austral de 2009. Los datos de movimiento del barco (cabeceo y balanceo fueron registrados in situ utilizando un clinómetro digital y luego utilizados para estimar la pérdida teórica de sensibilidad del transductor y, finalmente, traducir esta pérdida en factores de corrección del coeficiente de dispersión por área náutica (NASC calculados para intervalos básicos de muestreo de 926 m de distancia por 10 m de profundidad. Los factores de corrección del NASC (NASC Cp fueron calculados

  5. Temporal variations in methane emissions from emergent aquatic macrophytes in two boreonemoral lakes.

    Science.gov (United States)

    Milberg, Per; Törnqvist, Lina; Westerberg, Lars M; Bastviken, David

    2017-07-01

    Methane (CH 4 ) emissions via emergent aquatic macrophytes can contribute substantially to the global CH 4 balance. We addressed temporal variability in CH 4 flux by using the static chamber approach to quantify fluxes from plots dominated by two species considered to differ in flux transport mechanisms ( Phragmites australis , Carex rostrata ). Temporal variability in daily mean emissions from early June to early October was substantial. The variable that best explained this variation was air temperature. Regular and consistent diel changes were absent and therefore less relevant to include when estimating or modelling CH 4 emissions. Methane emissions per m 2 from nearby plots were similar for Phragmites australis and Carex rostrata indicating that CH 4 production in the system influenced emissions more than the species identity. This study indicates that previously observed diel patterns and species-effects on emissions require further evaluation to support improved local and regional CH 4 flux assessments.

  6. Ação do estresse térmico na sobrevivência de mudas e produção de camomila originadas de sementes importadas e nacionais Action of termic stress on the survival and yield of chamomile plantlets from imported and national seeds

    Directory of Open Access Journals (Sweden)

    José Roberto P de Souza

    2006-06-01

    Full Text Available Avaliou-se o efeito de baixas temperaturas sobre a sobrevivência de mudas de camomila (originadas de sementes importadas e coletadas localmente e sobre a produtividade da matéria-prima vegetal. Quarenta mudas com 7 a 10 cm de altura foram transferidas para câmara de germinação, tipo BOD, de duas procedências, onde permaneceram por 24 h nas condições: 10 h, com luz e 15ºC; 8 h, sem luz e 10ºC; 3 h, sem luz e 5ºC; 3 h; sem luz e temperaturas de 0ºC (testemunha, -2ºC, -4ºC e -6ºC. As mudas de sementes local apresentaram maior número de plantas mortas quando aplicadas às temperaturas de -4 e -6ºC, do que as importadas. O número de flores por planta e o acúmulo de matéria seca de flores por planta foram 462% e 226% superior, respectivamente, nas plantas originadas de sementes local comparadas com as importadas. O tratamento térmico de -6ºC proporcionou retomada do crescimento e aumento da produção das plantas de camomila, contrastando com aquelas submetidas às temperaturas de -2ºC e -4ºC. Os extratos das flores das plantas originadas de sementes local e importada mostraram igual quantidade de quercetina (substância marcadora.In the present trial the effects of low temperatures on the surviving of chamomile seedlings originated from two different origins (local and imported seeds was evaluated on the development, yield and concentration of quercetin. Forty to 10 cm high seedlings from the two origins were transferred to a germination chamber (BOD during 24 hours and submitted to the conditions: 10 h with light and 15ºC; 8 h without light and 10ºC; 3 h without light and 5ºC; 3 h without light and temperatures ranging from 0 (check, -2, -4, and -6ºC. The seedlings from national seeds were more susceptible to the temperatures of -4 and -6ºC. There were 462% more flowers per plant and 226% more dry matter accumulation per plant in the plants originated from national seeds compared to the plantlets from imported seeds

  7. Phytoremediation of Pharmaceuticals - Preliminary Study

    OpenAIRE

    Kotyza, J. (Jan); Soudek, P. (Petr); Kafka, Z.; Vaněk, T. (Tomáš)

    2010-01-01

    Phytoremediation of selected pharmaceuticals (diclofenac, ibuprofen, and acetaminophen) using Armoracia rusticana and Linum usitatissimum cell cultures and by hydroponically cultivated Lupinus albus, Hordeum vulgaris, and Phragmites australis plants in laboratory conditions is described. During in vitro experiments, the best results for acetaminophen were achieved using Armoracia rusticana hairy root cultures, where 100% of the starting amount was removed from the media during eight days. To...

  8. Vegetation types alter soil respiration and its temperature sensitivity at the field scale in an estuary wetland.

    Directory of Open Access Journals (Sweden)

    Guangxuan Han

    Full Text Available Vegetation type plays an important role in regulating the temporal and spatial variation of soil respiration. Therefore, vegetation patchiness may cause high uncertainties in the estimates of soil respiration for scaling field measurements to ecosystem level. Few studies provide insights regarding the influence of vegetation types on soil respiration and its temperature sensitivity in an estuary wetland. In order to enhance the understanding of this issue, we focused on the growing season and investigated how the soil respiration and its temperature sensitivity are affected by the different vegetation (Phragmites australis, Suaeda salsa and bare soil in the Yellow River Estuary. During the growing season, there were significant linear relationships between soil respiration rates and shoot and root biomass, respectively. On the diurnal timescale, daytime soil respiration was more dependent on net photosynthesis. A positive correlation between soil respiration and net photosynthesis at the Phragmites australis site was found. There were exponential correlations between soil respiration and soil temperature, and the fitted Q10 values varied among different vegetation types (1.81, 2.15 and 3.43 for Phragmites australis, Suaeda salsa and bare soil sites, respectively. During the growing season, the mean soil respiration was consistently higher at the Phragmites australis site (1.11 µmol CO2 m(-2 s(-1, followed by the Suaeda salsa site (0.77 µmol CO2 m(-2 s(-1 and the bare soil site (0.41 µmol CO2 m(-2 s(-1. The mean monthly soil respiration was positively correlated with shoot and root biomass, total C, and total N among the three vegetation patches. Our results suggest that vegetation patchiness at a field scale might have a large impact on ecosystem-scale soil respiration. Therefore, it is necessary to consider the differences in vegetation types when using models to evaluate soil respiration in an estuary wetland.

  9. Application of Hyperspectal Techniques to Monitoring & Management of Invasive Plant Species Infestation

    Science.gov (United States)

    2008-01-09

    Scirpus olnei, S. robustus, Hibiscus palustris, Eryngium virginianum. 1. Common reed (Phragmites australis) - large cane or bamboo-like grass...Index 2 760 695 R R plant stress status Zarco-Tejada (1998) PI3, Pigment Index 3 690 440 R R vegetation health , based on chlorophyll fluorescence...ratios Lichtenthaler et al. (1996) PI4, Pigment Index 4 740 440 R R vegetation health , based on chlorophyll fluorescence ratios

  10. Long-term ionizing radiation impact on seed progeny of common reed in water bodies of the Chernobyl exclusive zone

    International Nuclear Information System (INIS)

    Shevtsova, N.L.; Yavnyuk, A.A.; Gudkov, D.Yi.

    2012-01-01

    Results of the investigation of common reed's (Phragmites australis (Trin) Ex. Steud.) biological characteristics under conditions of long-term ionizing radiation impact are represented. Indices of seeds' vitality and disorders are analyzed. Low vitality indices, significant ontogenesis disorders, and high percent of abnormalities of germs are determined in water bodies, where littoral plants receive the absorbed dose in a low-dose range of 1-12 cGy year -1 .

  11. Subsurface flow constructed wetlands for the treatment of wastewater from different sources. Design and operation

    OpenAIRE

    Torrens Armengol, Antonina

    2016-01-01

    The aim of the thesis is to examine the viability of the subsurface constructed wetlands for the treatment of wastewater derived from three different sources (treatment ponds, pig farms and car wash facilities), and to evaluate the influence of design (size, type and depth of media, presence of Phragmites australis) and operational parameters (hydraulic load, dosing and feeding modes) on treatment efficiency and hydraulic behavior. Several studies were done in the framework of different ...

  12. Treating urban sewage using constructed wetlands; Depuracion de aguas residuales urbanas mediante humedales contruidos

    Energy Technology Data Exchange (ETDEWEB)

    Garcia, J. [ETS Camins, Canals i Ports. UPC. Barcelona (Spain); Ruiz, A. [Biologa. Barcelona. (Spain); Junqueras, X. [Biologo. Barcelona (Spain)

    1997-09-01

    Constructed wetlands are a low-cost alternative for treating sewage from small urbanized areas. The ``Can Massaguer`` children`s holiday home has a 230 m``2 subsurface flow wetland for secondary treatment of the sewage generated by 130 people. The system comprises two porous substrate beds with macrophytes (ditch reed, Phragmites australis) and entry and exit units. Its high purification performance and nil running costs make it ideal for treating wastewaters from small built-up areas. (Author)

  13. Vegetation Types Alter Soil Respiration and Its Temperature Sensitivity at the Field Scale in an Estuary Wetland

    Science.gov (United States)

    Han, Guangxuan; Xing, Qinghui; Luo, Yiqi; Rafique, Rashad; Yu, Junbao; Mikle, Nate

    2014-01-01

    Vegetation type plays an important role in regulating the temporal and spatial variation of soil respiration. Therefore, vegetation patchiness may cause high uncertainties in the estimates of soil respiration for scaling field measurements to ecosystem level. Few studies provide insights regarding the influence of vegetation types on soil respiration and its temperature sensitivity in an estuary wetland. In order to enhance the understanding of this issue, we focused on the growing season and investigated how the soil respiration and its temperature sensitivity are affected by the different vegetation (Phragmites australis, Suaeda salsa and bare soil) in the Yellow River Estuary. During the growing season, there were significant linear relationships between soil respiration rates and shoot and root biomass, respectively. On the diurnal timescale, daytime soil respiration was more dependent on net photosynthesis. A positive correlation between soil respiration and net photosynthesis at the Phragmites australis site was found. There were exponential correlations between soil respiration and soil temperature, and the fitted Q 10 values varied among different vegetation types (1.81, 2.15 and 3.43 for Phragmites australis, Suaeda salsa and bare soil sites, respectively). During the growing season, the mean soil respiration was consistently higher at the Phragmites australis site (1.11 µmol CO2 m−2 s−1), followed by the Suaeda salsa site (0.77 µmol CO2 m−2 s−1) and the bare soil site (0.41 µmol CO2 m−2 s−1). The mean monthly soil respiration was positively correlated with shoot and root biomass, total C, and total N among the three vegetation patches. Our results suggest that vegetation patchiness at a field scale might have a large impact on ecosystem-scale soil respiration. Therefore, it is necessary to consider the differences in vegetation types when using models to evaluate soil respiration in an estuary wetland. PMID:24608636

  14. The Contemporary Land Mammals of Egypt (Including Sinai).

    Science.gov (United States)

    1980-08-15

    dactylifera), Tamarix nilotica, and mats of Imperata cylin- drica and Juncus rigidus. The last, together with T nilotica, choke the salty stream bed of Wadi...bipinnata and Imperata cylindrical occur along the canal banks. Marshy areas are dominated byJuncus rigidus and Phragmites australis. Alhagi mannifera...mannifera and stands of Panicum turgidum, Imperata cylindrica, and l)esmostachya bi- pinnata occur on the deeper sand sheets. Trees (Maerua crassifolia

  15. Regeneration of begonia plantlets by direct organogenesis | Mendi ...

    African Journals Online (AJOL)

    African Journal of Biotechnology. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 8, No 9 (2009) >. Log in or Register to get access to full text downloads.

  16. Genetic stability among date palm plantlets regenerated from petiole ...

    African Journals Online (AJOL)

    The regeneration capacity between the shoot tip and petiole explants that excised from two date palm (Phoenix dactylifera L.) cutivar namely Unknown and Ferhi was compared. It was noted that the shoot tip explants started to swill after several subculture when placed on Murashige and Skoog (MS) medium supplemented ...

  17. Effect of plant growth regulators, explants type and efficient plantlet ...

    African Journals Online (AJOL)

    use

    2011-12-05

    Dec 5, 2011 ... Plant Pathology, Tissue Culture and Biotechnology Laboratory, Department of Botany,. University of ... variability in response to growth regulators. In vitro rooting ..... an adult tree Wrightia tomentosa through enhanced axillary.

  18. High frequency induction of somatic embryos and plantlet ...

    African Journals Online (AJOL)

    PRECIOUS

    2009-11-16

    Nov 16, 2009 ... plant, Hygrophila spinosa through direct somatic embryogenesis from nodal explants excised from 4 week old ... medicinal purposes further curbs propagation via seed. Plant tissue ... buted a great deal of information for the genetic, morpho- ..... analysis of peroxidase in cultured lettuce (Lactuca sativa L.).

  19. Regeneration of plantlets from unpollinated ovary cultures of ...

    African Journals Online (AJOL)

    Tsega

    2013-09-25

    Sep 25, 2013 ... 3Ethiopian Institute of Agricultural Research (EIAR): Holetta ... cultured unpollinated ovaries, 1,100 embryonic tissues (7.6 %) and 75 regenerants were obtained. The ... viral diseases, insect pests and increasing human popu-.

  20. one step method of plantlet regeneration in trichosanthes dioica roxb

    African Journals Online (AJOL)

    User

    2011-01-03

    Jan 3, 2011 ... Tissue Culture Laboratory, Department of Botany, Patna University, Patna, India. E-mail: rashmi0911@gmail.com. Accepted 23 December, 2010. In order to reduce the cost and time of in vitro raised plants of Trichosanthes dioica Roxb., a minimal .... 1951), tomato juice (Nitsch, 1951; Straus and La Rue,.

  1. Enhancement of Diosgenin Production in Plantlet and Cell Cultures ...

    African Journals Online (AJOL)

    HSCCC),. Sephadex LH-20 chromatography and preparative .... phase of acetonitrile-water (90:10, v/v) at a flow rate of 1 mL/min at 30 ºC, and an LC ..... Spirobisnaphthalenes from fungi and their biological activities. Mini-Rev Med Chem 2010; 10: ...

  2. Responses of macropropagated Musa plantlets to transient moisture ...

    African Journals Online (AJOL)

    Moisture stress causes serious devastating effects on crops ranging from retarded growth, tissue desiccation, and severe yield drop to outright death. In this study, the efficacy of six nursery substrates formulated in varying volume ratios of composted plantain fruit/bunch wastes (PFW), sawdust (SD), poultry manure (PM) and ...

  3. Identification of phytochemical components of aloe plantlets by gas ...

    African Journals Online (AJOL)

    MRT Pack 20 DVDs

    2013-12-04

    Dec 4, 2013 ... mented with 0.5 mgl-1 benzyl adenine + 0.5 mgl-1 α-naphthalene acetic acids into ... 5973) coupled to H.P. gas chromatograph (model 6890) equipped .... effects of Aloe vera leaf gel extract on oxidative stress in rats treated.

  4. Crescimento de mudas micropropagadas de bananeira aclimatizadas nas condições da Amazônia Sul Ocidental sob a influência de diferentes substratos e recipientes Growth of micropropagated banana plantlets acclimatized on conditions of South West Amazon under the influence of different substrates and recipients

    Directory of Open Access Journals (Sweden)

    Janiffe Peres de Oliveira

    2008-06-01

    . The aim of this work was to evaluate the growth of micropropagated banana plantlets during the acclimatization under the influence of different substrates and recipients on conditions of South West Amazon. Shoots of banana, cv. Grand Naine, were rooted in MS medium, being the rooting plants transferred to a nursery, in two types of plastic dibble tubes (115 cm³ and 180 cm³ and six different substrates formulated from different portions of soil, carbonized rice hulls and bovine manure. Evaluations of plant survival, height of the aerial part and pseudostem diameter were carried out each fifteen days, and at the end of 75 days, fresh and dry mass for the roots and aerial parts of the plants were also determined. It was verified that the plant survival was not influenced by the use of the 115 cm³ or 180 cm³ plastic dibble tubes. However, acclimatization accomplished in the 180 cm³ plastic dibble tubes provided larger growth in height and pseudostem diameter of the plants and, consequently, larger accumulation of fresh and dry mass of roots and aerial parts, when compared to the 115 cm³ plastic dibble tubes. Among the substrates, it was observed that bovine manure was fundamental as substrate component to obtain the best results.

  5. Isolation and characterization of microsatellite loci for Phragmites ...

    Indian Academy of Sciences (India)

    enriched library was constructed using the method described by Yu et al. (2009). A total of ... used a 20-μL PCR reaction system which contained 50 ng of genomic DNA, 0.2 ..... Published on the Web: 30 October 2013. Journal of Genetics, Vol ...

  6. Water level, vegetation composition, and plant productivity explain greenhouse gas fluxes in temperate cutover fens after inundation

    Science.gov (United States)

    Minke, Merten; Augustin, Jürgen; Burlo, Andrei; Yarmashuk, Tatsiana; Chuvashova, Hanna; Thiele, Annett; Freibauer, Annette; Tikhonov, Vitalij; Hoffmann, Mathias

    2016-07-01

    Peat extraction leaves a land surface with a strong relief of deep cutover areas and higher ridges. Rewetting inundates the deep parts, while less deeply extracted zones remain at or above the water level. In temperate fens the flooded areas are colonized by helophytes such as Eriophorum angustifolium, Carex spp., Typha latifolia or Phragmites australis dependent on water depth. Reeds of Typha and Phragmites are reported as large sources of methane, but data on net CO2 uptake are contradictory for Typha and rare for Phragmites. Here, we analyze the effect of vegetation, water level and nutrient conditions on greenhouse gas (GHG) emissions for representative vegetation types along water level gradients at two rewetted cutover fens (mesotrophic and eutrophic) in Belarus. Greenhouse gas emissions were measured campaign-wise with manual chambers every 2 to 4 weeks for 2 years and interpolated by modelling. All sites had negligible nitrous oxide exchange rates. Most sites were carbon sinks and small GHG sources. Methane emissions generally increased with net ecosystem CO2 uptake. Mesotrophic small sedge reeds with water table around the land surface were small GHG sources in the range of 2.3 to 4.2 t CO2 eq. ha-1 yr-1. Eutrophic tall sedge - Typha latifolia reeds on newly formed floating mats were substantial net GHG emitters in the range of 25.1 to 39.1 t CO2 eq. ha-1 yr. They represent transient vegetation stages. Phragmites reeds ranged between -1.7 to 4.2 t CO2 eq. ha-1 yr-1 with an overall mean GHG emission of 1.3 t CO2 eq. ha-1 yr-1. The annual CO2 balance was best explained by vegetation biomass, which includes the role of vegetation composition and species. Methane emissions were obviously driven by biological activity of vegetation and soil organisms. Shallow flooding of cutover temperate fens is a suitable measure to arrive at low GHG emissions. Phragmites australis establishment should be promoted in deeper flooded areas and will lead to moderate, but

  7. Hurricane Activity and the Large-Scale Pattern of Spread of an Invasive Plant Species

    Science.gov (United States)

    Bhattarai, Ganesh P.; Cronin, James T.

    2014-01-01

    Disturbances are a primary facilitator of the growth and spread of invasive species. However, the effects of large-scale disturbances, such as hurricanes and tropical storms, on the broad geographic patterns of invasive species growth and spread have not been investigated. We used historical aerial imagery to determine the growth rate of invasive Phragmites australis patches in wetlands along the Atlantic and Gulf Coasts of the United States. These were relatively undisturbed wetlands where P. australis had room for unrestricted growth. Over the past several decades, invasive P. australis stands expanded in size by 6–35% per year. Based on tropical storm and hurricane activity over that same time period, we found that the frequency of hurricane-force winds explained 81% of the variation in P. australis growth over this broad geographic range. The expansion of P. australis stands was strongly and positively correlated with hurricane frequency. In light of the many climatic models that predict an increase in the frequency and intensity of hurricanes over the next century, these results suggest a strong link between climate change and species invasion and a challenging future ahead for the management of invasive species. PMID:24878928

  8. Silver nanoparticles uptake by salt marsh plants - Implications for phytoremediation processes and effects in microbial community dynamics.

    Science.gov (United States)

    Fernandes, Joana P; Mucha, Ana P; Francisco, Telmo; Gomes, Carlos Rocha; Almeida, C Marisa R

    2017-06-15

    This study investigated the uptake of silver nanoparticles (AgNPs) by a salt marsh plant, Phragmites australis, as well as AgNPs effects on rhizospheric microbial community, evaluating the implications for phytoremediation processes. Experiments were carried out with elutriate solution doped with Ag, either in ionic form or in NP form. Metal uptake was evaluated in plant tissues, elutriate solutions and sediments (by AAS) and microbial community was characterized in terms of bacterial community structure (evaluated by ARISA). Results showed Ag accumulation but only in plant belowground tissues and only in the absence of rhizosediment, the presence of sediment reducing Ag availability. But in plant roots Ag accumulation was higher when Ag was in NP form. Multivariate analysis of ARISA profiles showed significant effect of the absence/presence of Ag either in ionic or NP form on microbial community structure, although without significant differences among bacterial richness and diversity. Overall, P. australis can be useful for phytoremediation of medium contaminated with Ag, including with AgNPs. However, the presence of Ag in either forms affected the microbial community structure, which may cause disturbances in ecosystems function and compromise phytoremediation processes. Such considerations need to be address regarding environmental management strategies applied to the very important estuarine areas. The form in which the metal was added affected metal uptake by Phragmites australis and rhizosediment microbial community structure, which can affect phytoremediation. Copyright © 2017 Elsevier Ltd. All rights reserved.

  9. Macrophytes of the Grlište reservoir (Serbia: Fifteen years after its establishment

    Directory of Open Access Journals (Sweden)

    Stanković Ž.

    2009-01-01

    Full Text Available A large number of macrophytes, often in dense populations, have developed on the Grlište Reservoir, Serbia over a period of 15 years. Fast development of vegetation is a consequence of anthropogenic impact in lake management. The methodology used in this research covered 100% of the water body, including all areas with or without aquatic plants. The results indicate that plant communities are still in the early phase of development. This leaves space for future development of competitor macrophyte species (Najas marina, Eleocharis palustris, Typha latifolia, Typha angustifolia, Phragmites australis, etc. capable of endangering stability of the lake, which will tend toward eutrophication.

  10. Vertical flow soil filter for the elimination of micro pollutants from storm and waste water

    DEFF Research Database (Denmark)

    Janzen, Niklas; Banzhaf, Stefan; Scheytt, Traugott

    2009-01-01

    A technical scale activated soil filter has been used to study the elimination rates of diverse environmentally relevant micro pollutants from storm and waste water. The filter was made of layers of peat, sand and gravel. The upper (organic) layer was planted with reed (phragmites australis......) to prevent clogging and was spiked with activated sludge to enhance microbial biomass and biodegradation potential. Compounds used as UV filters, antioxidants or plasticizers, namely 4-methylbenzylidene camphor (4-MBC), benzophenone-3 (BP-3), butylated hydroxytoluene (BHT), N-butylbenzenesulfonamide (NBBS...

  11. Land degradation and halophytic plant diversity of milleyha wetland ecosystem (samandag-hatay), Turkey

    International Nuclear Information System (INIS)

    Altay, V.

    2012-01-01

    Investigations were undertaken during 2010-2011 to study effect of human induced land degradation on structure of some halophytic plant communities. Over all 183 taxa of vascular plant were recorded. Out of these 76 were of typical halophytes. The dominant plant taxa were; Phragmites australis, Halimione portulacoides and Bolboschoenus maritimus. The threatened categories of these taxa were identified from the Red Data Book of Turkey together with their distribution. The impact of degradation on the habitats due to land use for agriculture, organic and inorganic waste disposal and housing for tourisitc purposes were identified and conservation measures were outlined in this study. (author)

  12. Minimizing the impacts of livestock. Treatment of slurry and water reuse on farms; Minimizacion de los impactos de la ganaderia. Tratamientos del purin y reutilizacion

    Energy Technology Data Exchange (ETDEWEB)

    Caballero, A.; Faz, A.; Bautista Lobera, J.; Palop, A

    2009-07-01

    In this study, wastewater from farms was treated using constructed wetlands, in a experimental farm, CIFEA, located in Lorca (Murcia), which is an important pig production area (southeast Spain). We have used horizontal sub-surface flow constructed wetlands with Phragmites australis, adapted to the semiarid climate of the area. We have obtained important removals of electrical conductivity by 28 %, total suspended solids by 93%, sedimentable solids by 100%, chemical oxygen demand by 87%, total phosphorus by 85%, copper by 92% and total nitrogen by 81%. (Author) 5 refs.

  13. Factorial's composition of Lake Abha, Southwestern Saudi Arabia

    International Nuclear Information System (INIS)

    El-Beheiry, M.A.H

    2007-01-01

    The study analyzes the vegetation along Lake Abha in Southwestern Saudi Arabia. A total of 42 plant species were recorded. The annuals decrease and the biennials and perennials increase along the moisture gradient form the terraces to the free-water zone. Six vegetation clusters were identified. The most important are clusters which were identified by the presence of the following species: Phragmites australis, Juncus punctorius, Typha domingensis, Cyperus rotundus, Datura innoxia, Cynodon dactylon, Cornulaca monacantha and Potamogeton nododsus. Each of these communities has been analyzed by classification and ordination techniques and its habitat described and discussed. (author)

  14. The distribution and accumulation of chromium in the water, sediment and macrophytes of Skadar lake

    Directory of Open Access Journals (Sweden)

    Kastratović Vlatko

    2016-01-01

    Full Text Available The aquatic macrophytes Phragmites australis (Cav. Trin. ex Steud., Ceratophyllum demersum L., and Lemna minor L. were used as bioindicator plant species in order to define contamination level by Cr in Skadar lake (Montenegro. Plants, water and sediments were tested for the content of Cr at six locations around Lake Skadar during four periods in 2011. The content of Cr in the examined sediment was in the range of 35.6-127 mg/kg dry weight. The largest proportion of detected Cr (50.6% was associated with the oxidizable phase in the form of organic complexes. The concentration of Cr in the studied macrophytes declined in the following order: C. demersum > P. australis > L. minor. The highest average content of Cr was detected in the leaf of C. demersum (11.4 mg/kg in April.

  15. Potential of Root Exudates from Wetland Plants and Their Potential Role for Denitrification and Allelopathic Interactions

    DEFF Research Database (Denmark)

    Zhai, Xu

    Root exudates from wetland plants have both positive and negative interactions among microbe, plants and ecosystems. Wetland species releasing organic carbon into the rhizosphere for providing energy to denitrifying bacteria fuel denitrification for removal nitrogen in subsurface flow constructed...... wetlands. Furthermore, environmental factors such as temperature and light-regime affect the photosynthetic carbon fixation, which continuously influence the compositions and quantity of root exudates released into rhizosphere. Conversely, root exudates from invasive species might contain some phytotoxic...... chemicals to suppress the growth of native species. Phragmites australis is recognized as the most invasive species in wetland ecosystems in North America, and allelopathy has been reported to be involved in the invasion success of the introduced exotic P. australis. The composition of the root exudates may...

  16. Homogenous stands of a wetland grass living in heavy metal polluted wetlands harbor diverse consortia of arbuscular mycorrhizal fungi.

    Science.gov (United States)

    Ban, Yihui; Jiang, Yinghe; Li, Meng; Zhang, Xiangling; Zhang, Shiyang; Wu, Yang; Xu, Zhouying

    2017-08-01

    Over the last three decades, the presence of arbuscular mycorrhizal (AM) fungi in wetland habitats had received increased attention, however, their distribution and functions have not been studied intensively. Using Illumina sequencing technology, we examined the AM fungal communities in roots of Phragmites australis living in 3 heavy metals (HMs) polluted wetlands located in Hubei Province, China. A total of 258 operational taxonomic units (OTUs) from 235,213 sequences affiliated with 6 Glomeromycota families (Glomeraceae, Paraglomeraceae, Claroideoglomeraceae, Ambisporaceae, Archaeosporaceae, and Diversisporaceae) were obtained, with Glomeraceae and Paraglomeraceae being the most and second-most dominant family, respectively. P. australis living in the HMs polluted wetlands harbored diverse AM fungi, including many non-recorded species in upland habitats, and the OTU number which we obtained in this study was higher than most of the records of upland habitats. Dry and waterlogged samples had common OTUs, however, AM fungal communities at different levels in dry and corresponding waterlogged P. australis roots were significant different. In addition, results from this study suggested that a preemption (geometric model) species abundance distributions (SAD), which might due to the distinctive features, e.g. heavy overdominance and difference in the most dominant taxon of each sample, was observed across AM fugal taxa in P. australis roots of the 3 HMs polluted wetlands. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Simulated herbivory and vegetation dynamics in coal slurry ponds reclaimed as wetlands

    Energy Technology Data Exchange (ETDEWEB)

    Mathis, M.J.; Middleton, B.A. [Southern Illinois University, Carbondale, IL (USA). Dept. of Plant Biology

    1999-12-01

    The biodiversity of coal slurry ponds can be inhibited at least in part, by dense stands of Phragmites australis. In this study, it is demonstrated that species richness can be increased in coal slurry ponds if the dominant species (P. Australis and Typha latifolia) are removed and that underwater herbivory simulated by cutting will kill emergents. The study was conducted in the greenhouse and the field in both flooded and drawndown conditions. In a reclaimed coal pond at Pyramid State Park, Illinois, neither P. australis nor T. Latifolia survived cutting underwater, but all of the uncut plants survived. Regrowth measured as total biomass of stems was less among flooded versus freely drained plants (0.3 and 2.6 g biomass, respectively). Cut versus uncut plants, combining freely drained and flooded, had less below-ground biomass (99.4 and 254.4 g, respectively). In an unreclaimed coal slurry pond with monospecific stands of P. Australis, plant species richness increased in cut plots as compared to uncut plots (29 vs 2 species, respectively) between March and September, 1995. This study demonstrated that species richness can be increased in coal ponds by mechanical cutting and this potentially by herbivory; however, the additional species were mostly exotics.

  18. Hydrological Conditions Affect the Interspecific Interaction between Two Emergent Wetland Species

    Directory of Open Access Journals (Sweden)

    Jian Zhou

    2018-01-01

    Full Text Available Hydrological conditions determine the distribution of plant species in wetlands, where conditions such as water depth and hydrological fluctuations are expected to affect the interspecific interactions among emergent wetland species. To test such effects, we conducted a greenhouse experiment with three treatment categories, interspecific interaction (mixed culture or monoculture, water depth (10 or 30 cm depth, and hydrological fluctuation (static or fluctuating water level, and two common emergent wetland plant species, Scirpus planiculumis Fr. (Cyperaceae and Phragmites australis var. baiyangdiansis (Gramineae. An increase in the water depth significantly restrained the growth of both S. planiculumis and P. australis, while hydrological fluctuations did not obviously alter the growth of either species. In addition, both water depth and hydrological fluctuations significantly affected the interspecific interaction between these two wetland species. P. australis benefited from interspecific interaction under increasing water depth and hydrological fluctuations, and the RII values were clearly positive for plants grown at a water depth that fluctuated around 30 cm. The results may have some implications for understanding how S. planiculumis and P. australis, as well as wetland communities, respond to the natural variation or human modification of hydrological conditions.

  19. Role of plants in nitrogen and sulfur transformations in floating hydroponic root mats: A comparison of two helophytes.

    Science.gov (United States)

    Saad, Rania A B; Kuschk, Peter; Wiessner, Arndt; Kappelmeyer, Uwe; Müller, Jochen A; Köser, Heinz

    2016-10-01

    Knowledge about the roles helophytes play in constructed wetlands (CWs) is limited, especially regarding their provision of organic rhizodeposits. Here, transformations of inorganic nitrogen and sulfur were monitored in a CW variety, floating hydroponic root mat (FHRM), treating synthetic wastewater containing low concentration of organic carbon. Two helophytes, Phragmites australis and Juncus effusus, were compared in duplicates. Striking differences were found between the FHRM of the two helophytes. Whereas ammonium was removed in all FHRMs to below detection level, total nitrogen of 1.15 ± 0.4 g m(-2) d(-1) was removed completely only in P. australis systems. The mats with J. effusus displayed effective nitrification but incomplete denitrification as 77% of the removed ammonium-nitrogen accumulated as nitrate. Furthermore, the P. australis treatment units showed on average 3 times higher sulfate-S removal rates (1.1 ± 0.45 g m(-2) d(-1)) than the systems planted with J. effusus (0.37 ± 0.29 g m(-2) d(-1)). Since the influent organic carbon was below the stoichiometric requirement for the observed N and S transformation processes, helophytes' organic rhizodeposits apparently contributed to these transformations, while P. australis provided about 6 times higher bioavailable organic rhizodeposits than J. effusus. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Competition from native hydrophytes reduces establishment and growth of invasive dense-flowered cordgrass (Spartina densiflora

    Directory of Open Access Journals (Sweden)

    Ahmed M. Abbas

    2015-10-01

    Full Text Available Experimental studies to determine the nature of ecological interactions between invasive and native species are necessary for conserving and restoring native species in impacted habitats. Theory predicts that species boundaries along environmental gradients are determined by physical factors in stressful environments and by competitive ability in benign environments, but little is known about the mechanisms by which hydrophytes exclude halophytes and the life history stage at which these mechanisms are able to operate. The ongoing invasion of the South American Spartina densiflora in European marshes is causing concern about potential impacts to native plants along the marsh salinity gradient, offering an opportunity to evaluate the mechanisms by which native hydrophytes may limit, or even prevent, the expansion of invasive halophytes. Our study compared S. densiflora seedling establishment with and without competition with Phragmites australis and Typha domingensis, two hydrophytes differing in clonal architecture. We hypothesized that seedlings of the stress tolerant S. densiflora would be out-competed by stands of P. australis and T. domingensis. Growth, survivorship, biomass patterns and foliar nutrient content were recorded in a common garden experiment to determine the effect of mature P. australis and T. domingensis on the growth and colonization of S. densiflora under fresh water conditions where invasion events are likely to occur. Mature P. australis stands prevented establishment of S. densiflora seedlings and T. domingensis reduced S. densiflora establishment by 38%. Seedlings grown with P. australis produced fewer than five short shoots and all plants died after ca. 2 yrs. Our results showed that direct competition, most likely for subterranean resources, was responsible for decreased growth rate and survivorship of S. densiflora. The presence of healthy stands of P. australis, and to some extent T. domingensis, along river channels

  1. Biology, Management and Utilization of Common Reed Pharagnites australis.

    Science.gov (United States)

    1987-02-01

    Vrije Universiteit Waterways Experiment Staticn Department of Ecology and Ecotoxicology Environmental Laboratory P.O. Box 7161 P.O. Box 631 1007 MC...Madelijn van der Werff Vrije Universiteit Department of Ecology and Ecotoxicology Amsterdam and John W. Simmers and Stratford H. Kay Waterways...lowing and cutting 67 5.2.4. Active regulation of the water table 68 5.5. Nature conservation 68 5.4. Reed cultivation 69 Z.4. 1. Reed harvest 69 6

  2. Isolation of fucoxanthin and fatty acids analysis of Padina australis ...

    African Journals Online (AJOL)

    GREGORY

    2011-12-16

    Dec 16, 2011 ... flame-ionization detector (FID) and a capillary column (Omegawax-. 320; 30 m x 0.32 mm i.d.; Supelco, Bellefonte, PA). The carrier gas was helium at a flow ..... resulted in formation of nuclear condensation, which was clearly evident in inverted microscope. The effect of fucoxanthin disappeared on H1299 ...

  3. A radiological study of the kiwi (Apteryx australis mantelli)

    International Nuclear Information System (INIS)

    Beale, G.

    1985-01-01

    A kiwi chick has been examined at intervals for four and a half years since hatching. The rate of skeletal maturation has been analysed from radiographs (x-ray photographs). Other aspects of the skeleton have been assessed, particularly the cervical spine. The results suggest that the thorax of the ancestors of the kiwi was more cephalically placed than at present and that four vertebrae classified as cervical were then in the thorax and carried ribs. Even in the modern kiwi vestigal ribs can be seen on the fifteenth cervical vertebra. Studies of the gastrointestinal tract have been carried out on other kiwis using a barium solution with videotaping and radiographic procedure. Anatomical and physiological observations, e.g., temperature, pulse and respiration studies have been made. On those birds requiring anaesthesia the effects of anaesthetic agents have been recorded. Some blood analysis has been done and there are observations on air sacs, the diaphragm and eggs. There is an appendix detailing the radiographic procedures used and the total amount of radiation administered, which was not significantly greater than natural gamma radiation over the same period

  4. Seasonal biodiversity and ecological studies on the epiphytic microalgae communities in polluted and unpolluted aquatic ecosystem at Assiut, Egypt

    Directory of Open Access Journals (Sweden)

    Adam Mahmoud S.

    2017-12-01

    Full Text Available A qualitative and quantitative study on epiphytic microalgae was carried out seasonally from November 2015 to August 2016 to follow up their community structures on aquatic macrophytes related to some physico-chemical properties of two polluted and unpolluted water bodies at Assiut, Egypt. A total of 169 species related to 64 genera of epiphytic microalgae were recorded. The most dominant algal group was Bacillariophyceae (43.2%, followed by Chlorophyceae (34.91%, Cyanophyceae (20.71% and Euglenophyceae (1.18%. The total number of epiphytic algae fluctuated between 11.1 × 104 ind.g-1 plant dry wt. on Phragmites australis in summer at Nazlet Abdellah (polluted site and 10.02 × 107 ind.g-1 plant dry wt. on Myriophyllum spicatum in winter at El-Wasta (unpolluted site. Some epiphytic microalgae were dominant as Pseudanabaena limnetica, Calothrix braunii, Scenedesmus acutus, and Ulnaria ulna. Others were specific on certain macrophytes as Aphanocapsa thermalis and Ulothrix sp., which grow on Phragmites australis, while Synechocystis minuscula attached itself on Myriophyllum spicatum. Analysis of PERMANOVA showed that the most important factors that induced the variation in epiphytic microalgae were the temporal variation and host plant. Water temperature, pH, nitrate, chloride, phosphate and total dissolved salts were the highest abiotic factors correlated with the variation in composition of epiphytic microalgae.

  5. Modeling the Effect of Plants and Peat on Evapotranspiration in Constructed Wetlands

    Directory of Open Access Journals (Sweden)

    Florent Chazarenc

    2010-01-01

    Full Text Available Evapotranspiration (ET in constructed wetlands (CWs represents a major factor affecting hydrodynamics and treatment performances. The presence of high ET was shown to improve global treatment performances, however ET is affected by a wide range of parameters including plant development and CWs age. Our study aimed at modelling the effect of plants and peat on ET in CWs; since we hypothesized peat could behave like the presence of accumulated organic matter in old CWs. Treatment performances, hydraulic behaviour, and ET rates were measured in eight 1 m2 CWs mesocosm (1 unplanted, 1 unplanted with peat, 2 planted with Phragmites australis, 2 planted with Typha latifolia and 2 planted with Phragmites australis with peat. Two models were built using first order kinetics to simulate COD and TKN removal with ET as an input. The effect of peat was positive on ET and was related to the better growth conditions it offered to macrophytes. Removal efficiency in pilot units with larger ET was higher for TKN. On average, results show for COD a k20 value of 0.88 d-1 and 0.36 d-1 for TKN. We hypothesized that the main effect of ET was to concentrate effluent, thus enhancing degradation rates.

  6. Concentrations of heavy metals and plant nutrients in water, sediments and aquatic macrophytes of anthropogenic lakes (former open cut brown coal mines) differing in stage of acidification

    Energy Technology Data Exchange (ETDEWEB)

    Samecka-Cymerman, A. [Department of Ecology and Nature Protection, Wroclaw University, ul. Kanonia 6/8, 50-328 Wroclaw (Poland); Kempers, A.J. [Department of Biogeology, University of Nijmegen, Toernooiveld, 6525 ED Nijmegen (Netherlands)

    2001-12-17

    Concentration of heavy metals (Al, Ba, Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, Sr, V and Zn) as well as macronutrients (N, P, K, Ca, Mg, S) were measured in water, bottom sediments and plants from anthropogenic lakes in West Poland. The collected plants were: Phragmites australis, Potamogeton natans, Iris pseudoacorus, Juncus effusus, Drepanocladus aduncus, Juncus bulbosus, Phalaris arundinacea, Carex remota and Calamagrostis epigeios. Two reference lakes were sampled for Nymphaea alba, Phragmites australis, Schoenoplectus lacustris, Typha angustifolia and Polygonum hydropiper. These plants contained elevated levels of Cd, Co, Cr, Cu and Mn, and part of the plants contained in addition elevated levels of Mn, Fe, Pb, Ni and Zn. Analyses of water indicated pollution with sulfates, Cd, Co, Ni, Zn, Pb and Cu, and bottom sediments indicated that some of the examined lakes were polluted with Cd, Co and Cr. Strong positive correlations were found between concentrations of Co in water and in plants and between Zn in sediments and plants, indicating the potential of plants for pollution monitoring for this metal. Heavy metal accumulation seemed to be directly associated with the exclusion of Ca and Mg.

  7. Methane emissions from a freshwater marsh in response to experimentally simulated global warming and nitrogen enrichment

    DEFF Research Database (Denmark)

    Flury, Sabine; McGinnis, Daniel Frank; Gessner, Mark O.

    2010-01-01

    We determined methane (CH4) emissions in a field enclosure experiment in a littoral freshwater marsh under the influence of experimentally simulated warming and enhanced nitrogen deposition. Methane emissions by ebullition from the marsh composed of Phragmites australis were measured with funnel ...... to the atmosphere, even when they occupy only relatively small littoral areas. More detailed investigations are clearly needed to assess whether global warming and nitrogen deposition can have climate feedbacks by altering methane fluxes from these wetlands.  ......We determined methane (CH4) emissions in a field enclosure experiment in a littoral freshwater marsh under the influence of experimentally simulated warming and enhanced nitrogen deposition. Methane emissions by ebullition from the marsh composed of Phragmites australis were measured with funnel...... traps deployed in a series of enclosures for two 3 week periods. Diffusive fluxes were estimated on the basis of measured CH4 concentrations and application of Fick's law. Neither diffusive nor ebullitive fluxes of methane were significantly affected by warming or nitrate enrichment, possibly because...

  8. Wetland craft plants in KwaZulu-Natal: an ecological review of har­vesting impacts and implications for sustainable utilization

    Directory of Open Access Journals (Sweden)

    C. H. Traynor

    2010-07-01

    Full Text Available In South Africa, wetland plants have been used for centuries and they continue to be harvested for subsistence and commercial purposes. Fibres for crafts are collected by cutting the aboveground parts. KwaZulu-Natal is one of the major basket-producing regions in southern Africa and at least twenty-two species of wetland plants are harvested for crafts. A literature review of the harvested species revealed that the impacts of cutting have only been extensively investigated for Phragmites australis (Cav. Steud. and Juncus kraussii Hochst. The review suggested that, where plants display strong seasonal aboveground productivity patterns, cutting should take place after shoot senescence and before new shoot emergence to minimize damage to plants. Cutting in the short term could increase the density of green stems. However, in the long term in  Phragmites australis, it may deplete the rhizome reserves and reduce the density of useable (longer and thicker culms.The opportunity for sustainable harvests was investigated by considering the geographic distribution, whether species are habitat specific or not, and local population sizes of the craft plants. Juncus kraussii is of the greatest conservation concern.Ecologically sustainable wetland plant harv esting could contribute to the wise use of wetlands, an approach promoted nationally and internationally.

  9. A multi-adaptive framework for the crop choice in paludicultural cropping systems

    Directory of Open Access Journals (Sweden)

    Nicola Silvestri

    2017-03-01

    Full Text Available The conventional cultivation of drained peatland causes peat oxidation, soil subsidence, nutrient loss, increasing greenhouse gas emissions and biodiversity reduction. Paludiculture has been identified as an alternative management strategy consisting in the cultivation of biomass on wet and rewetted peatlands. This strategy can save these habitats and restore the ecosystem services provided by the peatlands both on the local and global scale. This paper illustrates the most important features to optimise the crop choice phase which is the crucial point for the success of paludiculture systems. A multi-adaptive framework was proposed. It was based on four points that should be checked to identify suitable crops for paludicultural cropping system: biological traits, biomass production, attitude to cultivation and biomass quality. The main agronomic implications were explored with the help of some results from a plurennial open-field experimentation carried out in a paludicultural system set up in the Massaciuccoli Lake Basin (Tuscany, Italy and a complete example of the method application was provided. The tested crops were Arundo donax L., Miscanthus×giganteus Greef et Deuter, Phragmites australis L., Populus×canadensis Moench. and Salix alba L. The results showed a different level of suitability ascribable to the different plant species proving that the proposed framework can discriminate the behaviour of tested crops. Phragmites australis L. was the most suitable crop whereas Populus×canadensis Moench and Miscanthus×giganteus Greef et Deuter (in the case of biogas conversion occupied the last positions in the ranking.

  10. Phytoremediation of pharmaceuticals--preliminary study.

    Science.gov (United States)

    Kotyza, Jan; Soudek, Petr; Kafka, Zdenĕk; Vanĕk, Toás

    2010-03-01

    Phytoremediation of selected pharmaceuticals (diclofenac, ibuprofen, and acetaminophen) using Armoracia rusticana and Linum usitatissimum cell cultures and by hydroponically cultivated Lupinus albus, Hordeum vulgaris, and Phragmites australis plants in laboratory conditions is described. During in vitro experiments, the best results for acetaminophen were achieved using Armoracia rusticana hairy root cultures, where 100% of the starting amount was removed from the media during eight days. Total removal of ibuprofen and diclofenac was achieved using a Linum usitatissimum suspension culture after one and six days, respectively. In the hydroponic arrangement, the best results were achieved for Lupinus, where acetaminophen was totally removed from media during two or four days in concentrations of 0.1 or 0.2 mM, respectively. The best effectiveness of ibuprofen removal (50% of starting amount) was found in case of Phragmites. Effectiveness of all tested plants for diclofenac removal was low. The best removal was achieved using Phragmites in the case of 0.2 mM concentration-67% of the starting amount and Hordeum for 0.1 mM starting concentration, 56%.

  11. Effects of Invasive-Plant Management on Nitrogen-Removal Services in Freshwater Tidal Marshes.

    Directory of Open Access Journals (Sweden)

    Mary Alldred

    Full Text Available Establishing relationships between biodiversity and ecosystem function is an ongoing endeavor in contemporary ecosystem and community ecology, with important practical implications for conservation and the maintenance of ecosystem services. Removal of invasive plant species to conserve native diversity is a common management objective in many ecosystems, including wetlands. However, substantial changes in plant community composition have the potential to alter sediment characteristics and ecosystem services, including permanent removal of nitrogen from these systems via microbial denitrification. A balanced assessment of costs associated with keeping and removing invasive plants is needed to manage simultaneously for biodiversity and pollution targets. We monitored small-scale removals of Phragmites australis over four years to determine their effects on potential denitrification rates relative to three untreated Phragmites sites and adjacent sites dominated by native Typha angustifolia. Sediment ammonium increased following the removal of vegetation from treated sites, likely as a result of decreases in both plant uptake and nitrification. Denitrification potentials were lower in removal sites relative to untreated Phragmites sites, a pattern that persisted at least two years following removal as native plant species began to re-colonize treated sites. These results suggest the potential for a trade-off between invasive-plant management and nitrogen-removal services. A balanced assessment of costs associated with keeping versus removing invasive plants is needed to adequately manage simultaneously for biodiversity and pollution targets.

  12. Effects of Invasive-Plant Management on Nitrogen-Removal Services in Freshwater Tidal Marshes.

    Science.gov (United States)

    Alldred, Mary; Baines, Stephen B; Findlay, Stuart

    2016-01-01

    Establishing relationships between biodiversity and ecosystem function is an ongoing endeavor in contemporary ecosystem and community ecology, with important practical implications for conservation and the maintenance of ecosystem services. Removal of invasive plant species to conserve native diversity is a common management objective in many ecosystems, including wetlands. However, substantial changes in plant community composition have the potential to alter sediment characteristics and ecosystem services, including permanent removal of nitrogen from these systems via microbial denitrification. A balanced assessment of costs associated with keeping and removing invasive plants is needed to manage simultaneously for biodiversity and pollution targets. We monitored small-scale removals of Phragmites australis over four years to determine their effects on potential denitrification rates relative to three untreated Phragmites sites and adjacent sites dominated by native Typha angustifolia. Sediment ammonium increased following the removal of vegetation from treated sites, likely as a result of decreases in both plant uptake and nitrification. Denitrification potentials were lower in removal sites relative to untreated Phragmites sites, a pattern that persisted at least two years following removal as native plant species began to re-colonize treated sites. These results suggest the potential for a trade-off between invasive-plant management and nitrogen-removal services. A balanced assessment of costs associated with keeping versus removing invasive plants is needed to adequately manage simultaneously for biodiversity and pollution targets.

  13. An assessment of genetic fidelity of in vitro grown plantlets of rose ...

    African Journals Online (AJOL)

    A simple and routine method for the analysis of somaclonal variation among tissue culture derived rose plants is a prerequisite for precise monitoring of quality control during rapid mass micropropagation. Random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) molecular marker techniques ...

  14. Protein as a sole source of nitrogen for in vitro grown tobacco plantlets

    Czech Academy of Sciences Publication Activity Database

    Synková, Helena; Hýsková, V.; Garčeková, K.; Křížová, S.; Ryšlavá, H.

    2016-01-01

    Roč. 60, č. 4 (2016), s. 635-644 ISSN 0006-3134 Institutional support: RVO:61389030 Keywords : glutamate-dehydrogenase * inorganic nitrogen * nitrate transport * organic nitrogen * amino-acids * metabolism * seedlings * leaves * roots * assimilation * ammonium * casein * chlorophyll fluorescence * nitrate * photosynthesis Subject RIV: EF - Botanics Impact factor: 1.551, year: 2016

  15. Selection of suitable propagation method for consistent plantlets production in Stevia rebaudiana (Bertoni)

    OpenAIRE

    Khalil, Shahid Akbar; Zamir, Roshan; Ahmad, Nisar

    2014-01-01

    Stevia rebaudiana (Bert.) is an emerging sugar alternative and anti-diabetic plant in Pakistan. That is why people did not know the exact time of propagation. The main objective of the present study was to establish feasible propagation methods for healthy biomass production. In the present study, seed germination, stem cuttings and micropropagation were investigated for higher productivity. Fresh seeds showed better germination (25.51–40%) but lost viability after a few days of storage. In o...

  16. Regeneration of plantlets under NaCl stress from NaN3 treated ...

    African Journals Online (AJOL)

    use

    2011-11-16

    Nov 16, 2011 ... plant regeneration under NaCl stressed conditions was assessed in some sugarcane ( ... cell is a useful work for the establishment of pure form of ... balance. The relative water contents (RWC) were calculated .... J. Life Sci.

  17. In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia

    Directory of Open Access Journals (Sweden)

    Almeida Weliton Antonio Bastos de

    2002-01-01

    Full Text Available Exogenous genes can be introduced in plants by genetic transformation techniques. However, an efficient tissue culture system with high rates of plant recovery is necessary for gene introduction. This work aimed to define organogenesis and plant regeneration protocols for sweet orange varieties Natal, Valencia and Hamlin (Citrus sinensis L. Osbeck and Rangpur lime (Citrus limonia L. Osbeck which can be used in plant transformation experiments. Seeds of which teguments were removed, were germinated in vitro and maintained in the dark for three weeks, followed by one week at 16-h photoperiod (40 µmol m-2 s-1 and 27 ± 2°C. Organogenesis induction was done by introducing epicotyl segments in MT medium with 25 g L-1 sucrose and different BAP concentrations. After adventitious bud growth, the shoots were transferred to MT medium with either NAA or IBA (1 mg L-1, or absence of auxin, for rooting. The best results were obtained with 1 mg L-1 BAP for bud induction and 1 mg L-1 IBA for rooting for all three sweet orange cultivars. The use of 0.5-2.5 mg L-1 BAP, followed by 1 mg L-1 IBA were the best growth regulator combinations for bud induction and rooting, respectively, for 'Rangpur' lime. The protocols presented in this work are suitable for associations with genetic transformation experiments for these cultivars.

  18. Gelling agents and culture vessels affect in vitro multiplication of banana plantlets.

    Science.gov (United States)

    Kaçar, Y A; Biçen, B; Varol, I; Mendi, Y Y; Serçe, S; Cetiner, S

    2010-03-09

    Agar is the most commonly used gelling agent in media for plant tissue culture. Because of the high price of tissue-culture-grade agar, attempts have been made to identify suitable alternatives. The type of culture vessel and lid also affects the gaseous composition inside the vessel as well as light penetration. In turn, the vessel affects growth parameters, such as shoot elongation, proliferation and fresh weight, as well as hyperhydric degradation processes. We examined the effects of different culture vessels, including commercial glass jars, magenta boxes, and disposable containers, as well as different gelling agents (agar-agar, Agargel, Phytagel, and plant agar) on the micropropagation of Dwarf Cavendish bananas in an effort to find a combination that yields large numbers of high-quality seedlings. The different culture vessels did not significantly affect seedling culture success. The medium significantly affected shoot weight. Phytagel resulted in the highest shoot weight (overall mean = 2.4 g), while agar, Agargel and plant agar resulted in 1.7, 2.2 and 2.2 g, respectively. Disposable container/Phytagel and Magenta/Agargel combinations yielded the highest shoot weights (2.9 and 3.0 g, respectively). Mean shoot length increased progressively with subculture (four subcultures were made). The highest mean shoot length was obtained with Phytagel and Agargel media (6.4 and 6.3 cm, respectively). Shoot number was significantly affected by medium only at subculture 4. Overall, the highest mean shoot length was obtained with the Magenta/Agargel combination (8.5 cm). Phytagel and plant agar gave higher mean shoot number than agar and Agargel (2.1, 2.1 and 1.7 and 1.9, respectively). The costs of the media and of the culture vessels need to be taken into account for final choice of the banana shoot culture system.

  19. Effect of abscisic acid on photosynthetic parameters during ex vitro transfer of micropropagated tobacco plantlets

    Czech Academy of Sciences Publication Activity Database

    Pospíšilová, Jana; Synková, Helena; Haisel, Daniel; Baťková, Petra

    2009-01-01

    Roč. 53, č. 1 (2009), s. 11-20 ISSN 0006-3134 R&D Projects: GA ČR GA522/07/0227 Institutional research plan: CEZ:AV0Z50380511 Keywords : carotenoids * chlorophyll contents * chlorophyll fluorescence Subject RIV: ED - Physiology Impact factor: 1.656, year: 2009

  20. Manipulating the physiological quality of in vitro plantlets and transplants of potato

    NARCIS (Netherlands)

    Mehari, T.

    2000-01-01

    In vitro techniques have been introduced in potato seed production systems in recent years. This research project aimed at studying the morphological and physiological changes in plants and crops in the last three phases of a seed production system that included an

  1. Rapid Screening of In-Vitro Regenerated Plantlets of Four Nigerian ...

    African Journals Online (AJOL)

    Prof. Ogunji

    Tissue culture technique provides a rapid means of studying plant-pathogen interaction in a controlled ... Keywords: cowpea, in-vitro regeneration, hormones, Fusarium wilt, protocols. .... solanacearum and evaluation of tomato in Nepal.

  2. Seeds and plantlets of Myrciaria dubia “camu-camu”: biometry, germination, and initial growth

    Directory of Open Access Journals (Sweden)

    Anderson E. Medina Bardales

    2014-06-01

    Full Text Available The objectives were to determine seed biometrics, recording cellular respiration in the imbibition process, evaluate the effect of inducing chemicals in germination and early growth analysis. Cellular respiration during imbibition was measured by reduction of methylene blue and Janus Green B. The inductor effect of five chemical compounds in germination was evaluated. Initial growth was recorded by stereoscopic. Results showed that the seeds have an average weight of 520 ± 172 mg, length of 13.50 ± 1.76 mm, width of 10.61 ± 1.14 mm, and thickness of 4.68 ± 0 62 mm. These variables showed significant correlations (r of 0.494 to 0.897 and p 90% and 65% respectively. Seedling initial growth consisted of six stages and occurred over a period of 22 days after radicle emergence. In conclusion, M. dubia seeds show wide variation in their biometrics (weight, length, width and thickness, but show significant correlations with each other. Likewise, seeds are metabolically active in imbibition process, and germination is induced by sodium nitrite and potassium cyanide. Furthermore, seedling initial growth to occur over a period of 22 days after emergence of the radicle and consists of six distinct stages.

  3. Parameters necessary for in vitro hydroponic pea plantlet flowering and fruiting

    Science.gov (United States)

    To date, the development of culture systems to stimulate the reproductive and fruiting processes in vitro has not been earnestly exploited. Nonetheless, the ability to promote sterile flowering and fruiting would have commercial applications in the agricultural, food and medical industries. Non-ir...

  4. Phytoremediation of explosives for laboratory to real application

    Energy Technology Data Exchange (ETDEWEB)

    Vanek, T.; Vavrikova, Z.; Podlipna, R. [Inst. of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague (Czech Republic). Dept. of Plant Tissue Cultures; Gerth, A. [BioPlanta GmbH, Leipzig (Germany)

    2006-07-01

    This paper discussed the phytoremediation of 2,4,6-trinitrotoluene (TNT) contaminated soil at former ammunition factories in the Czech Republic and Germany. The uptake, degradation, and distribution of the compounds in various plants were evaluated. Characterization and identification of TNT degradation products is difficult due to the insoluble fractions bound in the plant cells. Rheum palmatum culture was supplemented with naphthaleneacetic acid and casein hydrolysate. The plant species Phragmites australis and Typha latifolia were cultivated and supplemented with vitamins. Roots were induced on the hormone-free medium during a 4 to 5 week cultivation period. 2,4,6-trinitrotoluene, glyceroltrinitrate and pentaerytritoltetranitrate were obtained from an ammunition factory. Explosives were added into the cultivation medium at a concentration of 50 mg/l during the first part of the experiment, as well as repeatedly at 10 and 50 mg/l during the second part of the experiment. The uptake of TNT by R. palmatum was determined over 24 hours. The initial concentration of TNT decreased to 50 per cent within half an hour. No TNT could be detected after 6 hours. At least 90 per cent of TNT was taken up by the plant species Phragmites, Typha and Juncus in 10 days. Faster uptake was determined in plants with roots. Phragmites australis was recommended as the best candidate for practical application as it forms a prolific biomass necessary for efficient biodegradation and is able to grow almost everywhere. A pilot-scale installation of constructed wetland containing nitroester explosives showed that the plants were able to clean water containing 270 mg/l of nitroesters and their by-products over a period of 30 days. 38 refs., 8 figs.

  5. Zinc accumulation in plant species indigenous to a Portuguese polluted site: relation with soil contamination.

    Science.gov (United States)

    Marques, Ana P G C; Rangel, António O S S; Castro, Paula M L

    2007-01-01

    The levels of zinc accumulated by roots, stems, and leaves of two plant species, Rubus ulmifolius and Phragmites australis, indigenous to the banks of a stream in a Portuguese contaminated site were investigated in field conditions. R. ulmifolius, a plant for which studies on phytoremediation potential are scarce, dominated on the right side of the stream, while P. australis proliferated on the other bank. Heterogeneous Zn concentrations were found along the banks of the stream. Zn accumulation in both species occurred mainly in the roots, with poor translocation to the aboveground sections. R. ulmifolius presented Zn levels in the roots ranging from 142 to 563 mg kg(-1), in the stems from 35 to 110 mg kg(-1), and in the leaves from 45 to 91 mg kg(-1), vs. average soil total Zn concentrations varying from 526 to 957 mg kg(-1). P. australis showed Zn concentrations in the roots from 39 to 130 mg kg(-1), in the stems from 31 to 63 mg kg(-1), and in the leaves from 37 to 83 mg kg(-1), for the lower average soil total Zn levels of 138 to 452 mg kg(-1) found on the banks where they proliferated. Positive correlations were found between the soil total, available and extractable Zn fractions, and metal accumulation in the roots and leaves of R. ulmifolius and in the roots and stems of P. australis. The use of R. ulmifolius and P. australis for phytoextraction purposes does not appear as an effective method of metal removing, but these native metal tolerant plant species may be used to reduce the effects of soil contamination, avoiding further Zn transfer to other environmental compartments.

  6. Development of autochthonous microbial consortia for enhanced phytoremediation of salt-marsh sediments contaminated with cadmium

    International Nuclear Information System (INIS)

    Teixeira, Catarina; Almeida, C. Marisa R.; Nunes da Silva, Marta; Bordalo, Adriano A.; Mucha, Ana P.

    2014-01-01

    Microbial assisted phytoremediation is a promising, though yet poorly explored, new remediation technique. The aim of this study was to develop autochthonous microbial consortia resistant to cadmium that could enhance phytoremediation of salt-marsh sediments contaminated with this metal. The microbial consortia were selectively enriched from rhizosediments colonized by Juncus maritimus and Phragmites australis. The obtained consortia presented similar microbial abundance but a fairly different community structure, showing that the microbial community was a function of the sediment from which the consortia were enriched. The effect of the bioaugmentation with the developed consortia on cadmium uptake, and the microbial community structure associated to the different sediments were assessed using a microcosm experiment. Our results showed that the addition of the cadmium resistant microbial consortia increased J. maritimus metal phytostabilization capacity. On the other hand, in P. australis, microbial consortia amendment promoted metal phytoextraction. The addition of the consortia did not alter the bacterial structure present in the sediments at the end of the experiments. This study provides new evidences that the development of autochthonous microbial consortia for enhanced phytoremediation of salt-marsh sediments contaminated with cadmium might be a simple, efficient, and environmental friendly remediation procedure. Capsule abstract: Development of autochthonous microbial consortia resistant to cadmium that enhanced phytoremediation by salt-marsh plants, without a long term effect on sediment bacterial diversity. - Highlights: • Cd resistant microbial consortia were developed and applied to salt-marsh sediments. • In Phragmites australis the consortia amendment promoted metal phytoextraction. • The consortia addition increased Juncus maritimus phytostabilization capacity. • No long term changes on the rhizosediment bacterial structure were observed

  7. Heavy metal content in ash of energy crops growing in sewage-contaminated natural wetlands: Potential applications in agriculture and forestry?

    International Nuclear Information System (INIS)

    Bonanno, Giuseppe; Cirelli, Giuseppe Luigi; Toscano, Attilio; Giudice, Rosa Lo; Pavone, Pietro

    2013-01-01

    One of the greatest current challenges is to find cost-effective and eco-friendly solutions to the ever increasing needs of modern society. Some plant species are suitable for a multitude of biotechnological applications such as bioenergy production and phytoremediation. A sustainable practice is to use energy crops to clean up polluted lands or to treat wastewater in constructed wetlands without claiming further arable land for biofuel production. However, the disposal of combustion by-products may add significant costs to the whole process, especially when it deals with toxic waste. This study aimed to investigate the possibility of recycling ash from energy biomass as a fertilizer for agriculture and forestry. In particular, the concentrations of Cd, Cr, Cu, Mn, Pb and Zn were analyzed in the plant tissues and corresponding ash of the grasses Phragmites australis and Arundo donax, collected in an urban stream affected by domestic sewage. Results showed that the metal concentration in ash is 1.5–3 times as high as the values in plant tissues. However, metal enriched ash showed much lower element concentrations than the legal limits for ash reutilization in agriculture and forestry. This study found that biomass ash from constructed wetlands may be considered as a potential fertilizer rather than hazardous waste. Energy from biomass can be a really sustainable and clean option not only through the reduction of greenhouse gas emissions, but also through ash recycling for beneficial purposes, thus minimizing the negative impacts of disposal. - Highlights: • Metal content in ash reflects the element concentrations in Phragmites australis and Arundo donax. • Metal enriched ash of both species may be recycled as fertilizers in agriculture and forestry. • Constructed wetlands may produce a large amount of plant ash-based fertilizers from P. australis and A. donax

  8. Treatment of sludge containing nitro-aromatic compounds in reed-bed mesocosms – Water, BOD, carbon and nutrient removal

    International Nuclear Information System (INIS)

    Gustavsson, L.; Engwall, M.

    2012-01-01

    Highlights: ► It is necessary to improve existing and develop new sludge management techniques. ► One method is dewatering and biodegradation of compounds in constructed wetlands. ► The result showed high reduction of all tested parameters after treatment. ► Plants improve degradation and Phragmites australis is tolerant to xenobiotics. ► The amount of sludge could be reduced by 50–70%. - Abstract: Since the mid-1970s, Sweden has been depositing 1 million ton d.w sludge/year, produced at waste water treatment plants. Due to recent legislation this practice is no longer a viable method of waste management. It is necessary to improve existing and develop new sludge management techniques and one promising alternative is the dewatering and treatment of sludge in constructed wetlands. The aim of this study was to follow reduction of organic carbon, BOD and nutrients in an industrial sludge containing nitro-aromatic compounds passing through constructed small-scale wetlands, and to investigate any toxic effect such as growth inhibition of the common reed Phragmites australis. The result showed high reduction of all tested parameters in all the outgoing water samples, which shows that constructed wetlands are suitable for carbon and nutrient removal. The results also showed that P. australis is tolerant to xenobiotics and did not appear to be affected by the toxic compounds in the sludge. The sludge residual on the top of the beds contained low levels of organic carbon and is considered non-organic and could therefore be landfilled. Using this type of secondary treatment method, the amount of sludge could be reduced by 50–70%, mainly by dewatering and biodegradation of organic compounds.

  9. Development of autochthonous microbial consortia for enhanced phytoremediation of salt-marsh sediments contaminated with cadmium

    Energy Technology Data Exchange (ETDEWEB)

    Teixeira, Catarina [Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR/CIMAR), Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto (Portugal); Laboratório de Hidrobiologia e Ecologia, Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Universidade do Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313 Porto (Portugal); Almeida, C. Marisa R.; Nunes da Silva, Marta [Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR/CIMAR), Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto (Portugal); Bordalo, Adriano A. [Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR/CIMAR), Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto (Portugal); Laboratório de Hidrobiologia e Ecologia, Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Universidade do Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313 Porto (Portugal); Mucha, Ana P., E-mail: amucha@ciimar.up.pt [Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR/CIMAR), Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto (Portugal)

    2014-09-15

    Microbial assisted phytoremediation is a promising, though yet poorly explored, new remediation technique. The aim of this study was to develop autochthonous microbial consortia resistant to cadmium that could enhance phytoremediation of salt-marsh sediments contaminated with this metal. The microbial consortia were selectively enriched from rhizosediments colonized by Juncus maritimus and Phragmites australis. The obtained consortia presented similar microbial abundance but a fairly different community structure, showing that the microbial community was a function of the sediment from which the consortia were enriched. The effect of the bioaugmentation with the developed consortia on cadmium uptake, and the microbial community structure associated to the different sediments were assessed using a microcosm experiment. Our results showed that the addition of the cadmium resistant microbial consortia increased J. maritimus metal phytostabilization capacity. On the other hand, in P. australis, microbial consortia amendment promoted metal phytoextraction. The addition of the consortia did not alter the bacterial structure present in the sediments at the end of the experiments. This study provides new evidences that the development of autochthonous microbial consortia for enhanced phytoremediation of salt-marsh sediments contaminated with cadmium might be a simple, efficient, and environmental friendly remediation procedure. Capsule abstract: Development of autochthonous microbial consortia resistant to cadmium that enhanced phytoremediation by salt-marsh plants, without a long term effect on sediment bacterial diversity. - Highlights: • Cd resistant microbial consortia were developed and applied to salt-marsh sediments. • In Phragmites australis the consortia amendment promoted metal phytoextraction. • The consortia addition increased Juncus maritimus phytostabilization capacity. • No long term changes on the rhizosediment bacterial structure were observed.

  10. Heavy metal content in ash of energy crops growing in sewage-contaminated natural wetlands: Potential applications in agriculture and forestry?

    Energy Technology Data Exchange (ETDEWEB)

    Bonanno, Giuseppe, E-mail: bonanno.giuseppe@unict.it [Department of Biological, Geological and Environmental Sciences, University of Catania, Via Longo 19, 95125, Catania (Italy); Cirelli, Giuseppe Luigi; Toscano, Attilio [Department of Agri-Food and Environmental Systems Management, University of Catania, Via Santa Sofia 100, 95123, Catania (Italy); Giudice, Rosa Lo; Pavone, Pietro [Department of Biological, Geological and Environmental Sciences, University of Catania, Via Longo 19, 95125, Catania (Italy)

    2013-05-01

    One of the greatest current challenges is to find cost-effective and eco-friendly solutions to the ever increasing needs of modern society. Some plant species are suitable for a multitude of biotechnological applications such as bioenergy production and phytoremediation. A sustainable practice is to use energy crops to clean up polluted lands or to treat wastewater in constructed wetlands without claiming further arable land for biofuel production. However, the disposal of combustion by-products may add significant costs to the whole process, especially when it deals with toxic waste. This study aimed to investigate the possibility of recycling ash from energy biomass as a fertilizer for agriculture and forestry. In particular, the concentrations of Cd, Cr, Cu, Mn, Pb and Zn were analyzed in the plant tissues and corresponding ash of the grasses Phragmites australis and Arundo donax, collected in an urban stream affected by domestic sewage. Results showed that the metal concentration in ash is 1.5–3 times as high as the values in plant tissues. However, metal enriched ash showed much lower element concentrations than the legal limits for ash reutilization in agriculture and forestry. This study found that biomass ash from constructed wetlands may be considered as a potential fertilizer rather than hazardous waste. Energy from biomass can be a really sustainable and clean option not only through the reduction of greenhouse gas emissions, but also through ash recycling for beneficial purposes, thus minimizing the negative impacts of disposal. - Highlights: • Metal content in ash reflects the element concentrations in Phragmites australis and Arundo donax. • Metal enriched ash of both species may be recycled as fertilizers in agriculture and forestry. • Constructed wetlands may produce a large amount of plant ash-based fertilizers from P. australis and A. donax.

  11. Evaluation of seasonal changes in methane flux in a wetland ecosystem using the Closed Geosphere Experiment Facility

    Science.gov (United States)

    Suzuki, S.; Inubushi, K.; Yokozawa, M.; Hara, T.; Nishidate, K.; Tsuga, S.; Tako, Y.; Nakamura, Y.

    2009-04-01

    To estimate CH4 emission from a wetland ecosystem to the atmosphere, seasonal change in CH4 flux was measured continuously in the Closed Geosphere Experiment Facility (CGEF). Plant-mediated transport is one of the important pathways for CH4 emission from Phragmites australis-dominated vegetation because most CH4 emission occurs through P. australis plant. The CGEF is equipped with a Geosphere Module (GM) and a Geosphere Material Circulation (GMC) system. The size of the GM is 5.8 m Ã- 8.7 m in ground area with an average height of 11.9 m, including the soil depth of 3.1 m. A wetland ecosystem dominated by P. australis was introduced into the GM. The CGEF can control air temperature and CO2 concentration in the GM automatically. Hourly CH4 flux from the wetland ecosystem can be calculated easily by measuring continuously the changes in CH4 concentration in air, air temperature and pressure in the GM. The method showed that monthly CH4 flux varied from 0.39 to 1.11 g C m-2 month-1 from April to November and the CH4 emission for the plant growing season (eight months) was 5.64 g C m-2. The CGEF has an advantage in studying total CH4 emission from soil to the atmosphere through plant-mediated transport, diffusion and ebullition because of the large size of the GM.

  12. Habitat-dependent changes in vigilance behaviour of Red-crowned Crane influenced by wildlife tourism.

    Science.gov (United States)

    Li, Donglai; Liu, Yu; Sun, Xinghai; Lloyd, Huw; Zhu, Shuyu; Zhang, Shuyan; Wan, Dongmei; Zhang, Zhengwang

    2017-11-30

    The Endangered Red-crowned Crane (Grus japonensis) is one of the most culturally iconic and sought-after species by wildlife tourists. Here we investigate how the presence of tourists influence the vigilance behaviour of cranes foraging in Suaeda salsa salt marshes and S. salsa/Phragmites australis mosaic habitat in the Yellow River Delta, China. We found that both the frequency and duration of crane vigilance significantly increased in the presence of wildlife tourists. Increased frequency in crane vigilance only occurred in the much taller S. salsa/P. australis mosaic vegetation whereas the duration of vigilance showed no significant difference between the two habitats. Crane vigilance declined with increasing distance from wildlife tourists in the two habitats, with a minimum distance of disturbance triggering a high degree of vigilance by cranes identified at 300 m. The presence of wildlife tourists may represent a form of disturbance to foraging cranes but is habitat dependent. Taller P. australis vegetation serves primarily as a visual obstruction for cranes, causing them to increase the frequency of vigilance behaviour. Our findings have important implications for the conservation of the migratory red-crowned crane population that winters in the Yellow River Delta and can help inform visitor management.

  13. Remediation of mercury-polluted soils using artificial wetlands.

    Science.gov (United States)

    García-Mercadoa, Héctor Daniel; Fernándezb, Georgina; Garzón-Zúñigac, Marco Antonio; Durán-Domínguez-de-Bazúaa, María Del Carmen

    2017-01-02

    Mexico's mercury mining industry is important for economic development, but has unfortunately contaminated soils due to open-air disposal. This case was seen at two sites in the municipality of Pinal de Amoles, State of Queretaro, Mexico. This paper presents an evaluation of mercury dynamics and biogeochemistry in two soils (mining waste soil) using ex-situ wetlands over 36 weeks. In soils sampled in two former mines of Pinal de Amoles, initial mercury concentrations were 424 ± 29 and 433 ± 12 mg kg -1 in La Lorena and San Jose, former mines, respectively. Typha latifolia and Phragmites australis were used and 20 reactors were constructed (with and without plants). The reactors were weekly amended with a nutrient solution (NPK), for each plant, at a pH of 5.0. For remediation using soils from San Jose 70-78% of mercury was removed in T. latifolia reactors and 76-82% in P. australis reactors, and for remediation of soils from La Lorena, mercury content was reduced by 55-71% using T. latifolia and 58-66% in P. australis reactors. Mercury emissions into the atmosphere were estimated to be 2-4 mg m -2 h -1 for both soils.

  14. Biogeochemical features of aquatic plants in the Selenga River delta

    Science.gov (United States)

    Shinkareva, Galina; Lychagin, Mikhail

    2014-05-01

    The Selenga River system provides more than a half of the Lake Baikal total inflow. The river collects a significant amount of pollutants (e.g. heavy metals) from the whole basin. These substances are partially deposited within the Selenga delta, and partially are transported further to the lake. A generous amount of aquatic plants grow in the delta area according to its favorable conditions. This vegetation works as a specific biofilter. It accumulates suspended particles and sorbs some heavy metals from the water. The study aimed to reveal the species of macrophytes which could be mostly important for biomonitoring according to their chemical composition. The field campaign took place in the Selenga River delta in July-August of 2011 (high water period) and in June of 2012 (low water period). 14 species of aquatic plants were collected: water starwort Callitriche hermaphroditica, small yellow pond lily Nuphar pumila, pondweeds Potamogeton crispus, P. pectinatus, P. friesii, broadleaf cattail Typha latifolia, hornwort or coontail Ceratophyllum demersum, arrowhead Sagittaria natans, flowering rush (or grass rush) Butomus umbellatus, reed Phragmites australis, parrot's feather Myriophyllum spicatum, the common mare's tail Hippuris vulgaris, Batrachium trichophyllum, canadian waterweed Elodea canadensis. The samples were dried, grinded up and digested in a mixture of HNO3 and H2O2. The chemical composition of the plant material was defined using ICP-MS and ICP-AES methods. Concentrations of Fe, Mn, Cr, Ni, Cu, B, Zn, V, Co, As, Mo, Pb, and U were considered. The study revealed that Potamogeton pectinatus and Myriophyllum spicatum concentrate elements during both high and low water periods. Conversely the Butomus umbellatus and Phragmites australis contain small amount of heavy metals. The reed as true grasses usually accumulates fewer amounts of elements than other macrophytes. To compare biogeochemical specialization of different species we suggest to use

  15. Effects of Spartina alterniflora invasion on the communities of methanogens and sulfate-reducing bacteria in estuarine marsh sediments

    Directory of Open Access Journals (Sweden)

    Jemaneh eZeleke

    2013-08-01

    Full Text Available The effect of plant invasion on the microorganisms of soil sediments is very important for estuary ecology. The community structures of methanogens and sulfate-reducing bacteria (SRB as a function of Spartina alterniflora invasion in Phragmites australis-vegetated sediments of the Dongtan wetland in the Yangtze River estuary, China, were investigated using 454 pyrosequencing and quantitative real-time PCR (qPCR of the methyl coenzyme M reductase A (mcrA and dissimilatory sulfite-reductase (dsrB genes. Sediment samples were collected from two replicate locations, and each location included three sampling stands each covered by monocultures of P. australis, S. alterniflora and both plants (transition stands, respectively. qPCR analysis revealed higher copy numbers of mcrA genes in sediments from S. alterniflora stands than P. australis stands (5- and 7.5-fold more in the spring and summer, respectively, which is consistent with the higher methane flux rates measured in the S. alterniflora stands (up to 8.01 ± 5.61 mg m-2 h-1. Similar trends were observed for SRB, and they were up to two orders of magnitude higher than the methanogens. Diversity indices indicated a lower diversity of methanogens in the S. alterniflora stands than the P. australis stands. In contrast, insignificant variations were observed in the diversity of SRB with the invasion. Although Methanomicrobiales and Methanococcales, the hydrogenotrophic methanogens, dominated in the salt marsh, Methanomicrobiales displayed a slight increase with the invasion and growth of S. alterniflora, whereas the later responded differently. Methanosarcina, the metabolically diverse methanogens, did not vary with the invasion of, but Methanosaeta, the exclusive acetate utilizers, appeared to increase with S. alterniflora invasion. In SRB, sequences closely related to the families Desulfobacteraceae and Desulfobulbaceae dominated in the salt marsh, although they displayed minimal changes with the S

  16. Tidal flushing restores the physiological condition of fish residing in degraded salt marshes.

    Directory of Open Access Journals (Sweden)

    Kimberly L Dibble

    Full Text Available Roads, bridges, and dikes constructed across salt marshes can restrict tidal flow, degrade habitat quality for nekton, and facilitate invasion by non-native plants including Phragmites australis. Introduced P. australis contributes to marsh accretion and eliminates marsh surface pools thereby adversely affecting fish by reducing access to intertidal habitats essential for feeding, reproduction, and refuge. Our study assessed the condition of resident fish populations (Fundulus heteroclitus at four tidally restricted and four tidally restored marshes in New England invaded by P. australis relative to adjacent reference salt marshes. We used physiological and morphological indicators of fish condition, including proximate body composition (% lipid, % lean dry, % water, recent daily growth rate, age class distributions, parasite prevalence, female gravidity status, length-weight regressions, and a common morphological indicator (Fulton's K to assess impacts to fish health. We detected a significant increase in the quantity of parasites infecting fish in tidally restricted marshes but not in those where tidal flow was restored to reduce P. australis cover. Using fish length as a covariate, we found that unparasitized, non-gravid F. heteroclitus in tidally restricted marshes had significantly reduced lipid reserves and increased lean dry (structural mass relative to fish residing in reference marshes. Fish in tidally restored marshes were equivalent across all metrics relative to those in reference marshes indicating that habitat quality was restored via increased tidal flushing. Reference marshes adjacent to tidally restored sites contained the highest abundance of young fish (ages 0-1 while tidally restricted marshes contained the lowest. Results indicate that F. heteroclitus residing in physically and hydrologically altered marshes are at a disadvantage relative to fish in reference marshes but the effects can be reversed through ecological

  17. The Effect of Restoration on Soil Respiration in an Urban Tidal Wetland in the Meadowlands, New Jersey

    Science.gov (United States)

    Schafer, K. V.; Kurepa, S.; Duman, T.; Scott, M.; Pechmann, I.; Vanderklein, D. W.

    2017-12-01

    The effect of wetland restoration on soil respiration in tidal brackish marshes has not been comprehensively studied. In New Jersey, common mitigation efforts come in the form of the removal of an invasive haplotype of Phragmites australis and replanting of native species, resulting in significant habitat disturbance. This study investigated the differences in soil respiration within and between areas covered with P. australis, Spartina alterniflora, and Spartina patens. We performed static chamber measurements of soil respiration using an infrared gas analyzer to measure CO2 fluxes in a natural site and a mitigated site in the Meadowlands of New Jersey. Daytime measurements were performed in 10 random locations in areas populated with each of the vegetation types, to represent the spatial heterogeneity of the wetland area, during summer 2017. Due to the nature of the wetland, vegetation had to be removed to uncover the soil. Prior to measuring exposed soil respiration, we therefore measured CO2 flux including the vegetation within the chamber, which allowed us to additionally calculate the respiration including the vegetation. Furthermore, we assessed direct respiration of green leaves with leaf gas exchange measurements. Combining these different methodologies and scales allow us to estimate the function of different components that contribute to total respiration from the wetland, and how they change spatially and temporally. Initial results showed that soil respiration in P. australis patches was much higher than in both Spartina species, however average vegetation respiration per unit mass was similar across all three. Vegetation respiration and soil respiration are of the same order of magnitude in all three species as well. Also, when respiration with and without vegetation was combined, P. australis showed a considerably higher flux.

  18. Marsh plant response to metals: Exudation of aliphatic low molecular weight organic acids (ALMWOAs)

    Science.gov (United States)

    Rocha, A. Cristina S.; Almeida, C. Marisa R.; Basto, M. Clara P.; Vasconcelos, M. Teresa S. D.

    2016-03-01

    Metal exposure is known to induce the production and secretion of substances, such as aliphatic low molecular weight organic acids (ALMWOAs), into the rhizosphere by plant roots. Knowledge on this matter is extensive for soil plants but still considerably scarce regarding marsh plants roots adapted to high salinity media. Phragmites australis and Halimione portulacoides, two marsh plants commonly distributed in European estuarine salt marshes, were used to assess the response of roots of both species, in terms of ALMWOAs exudation, to Cu, Ni and Cd exposure (isolated and in mixture since in natural environment, they are exposed to mixture of metals). As previous studies were carried out in unrealistic and synthetic media, here a more natural medium was selected. Therefore, in vitro experiments were carried out, with specimens of both marsh plants, and in freshwater contaminated with two different Cu, Ni and Cd concentrations (individual metal and in mixture). Both marsh plants were capable of liberating ALMWOAs into the surrounding medium. Oxalic, citric and maleic acids were found in P. australis root exudate solutions and oxalic and maleic acids in H. portulacoides root exudate solutions. ALMWOA liberation by both plants was plant species and metal-dependent. For instance, Cu affected the exudation of oxalic acid by H. portulacoides and of oxalic and citric acids by P. australis roots. In contrast, Ni and Cd did not stimulate any specific response. Regarding the combination of all metals, H. portulacoides showed a similar response to that observed for Cu individually. However, in the P. australis case, at high metal concentration mixture, a synergetic effect led to the increase of oxalic acid levels in root exudate solution and to a decrease of citric acid liberation. A correlation between ALMWOAs exudation and metal accumulation could not be established. P. australis and H. portulacoides are considered suitable metal phytoremediators of estuarine impacted areas

  19. Comparative study on effects of four energy plants growth on chemical fractions of heavy metals and activity of soil enzymes in copper mine tailings.

    Science.gov (United States)

    Zhang, Jie; Yang, Shiyong; Yang, Hongfei; Huang, Yongjie; Zheng, Liming; Yuan, Jing; Zhou, Shoubiao

    2018-05-12

    Four gramineous energy plants, Miscanthus sacchariflorus, M. floridulus, Phragmites australis, and Arundo donax were grown on copper tailings in the field for four years. Their phytoremediation potential was examined in terms of their effects on the fractions of heavy metals and soil enzyme activities. Results showed that plantation of these four gramineous plants has improved the proportion of organic material (OM)-binding fraction of heavy metals in copper tailings as a whole, and reduced the proportion of exchangeable and residual fractions. In particular, M. sacchariflorus growth improved significantly the proportion of the OM-binding fractions of Cu (1.73 times), Cd (1.71 times), Zn (1.18 times), and Pb (3.14 times) (P tailings to some extent. In particular, the activity of soil phosphatase and urease in planted tailings differed significantly from that of control (P tailings.

  20. Investigation of a Bio-Inspired Liner Concept

    Science.gov (United States)

    Koch, L. Danielle

    2017-01-01

    Four samples of natural reeds, Phragmites australis, were tested in the NASA Langley and Glenn Normal Incidence Impedance Tubes in order to experimentally determine the acoustic absorption coefficients as a function of frequency from 400 to 3000 Hz. Six samples that mimicked the geometry of the assemblies of natural reeds were also designed and additively manufactured from ASA thermoplastic and tested. Results indicate that structures can be manufactured of synthetic materials that mimic the geometry and the low frequency acoustic absorption of natural reeds. This accomplishment demonstrates that a new class of structures can now be considered for a wide range of industrial products that need thin, lightweight, broadband acoustic absorption effective at frequencies below 1000 Hz. Aircraft engine acoustic liners and aircraft cabin acoustic liners, in particular, are two aviation applications that might benefit from further development of this concept.