WorldWideScience

Sample records for permeate large metabolic

  1. Fermentation of lactose to ethanol in cheese whey permeate and concentrated permeate by engineered Escherichia coli.

    Science.gov (United States)

    Pasotti, Lorenzo; Zucca, Susanna; Casanova, Michela; Micoli, Giuseppina; Cusella De Angelis, Maria Gabriella; Magni, Paolo

    2017-06-02

    Whey permeate is a lactose-rich effluent remaining after protein extraction from milk-resulting cheese whey, an abundant dairy waste. The lactose to ethanol fermentation can complete whey valorization chain by decreasing dairy waste polluting potential, due to its nutritional load, and producing a biofuel from renewable source at the same time. Wild type and engineered microorganisms have been proposed as fermentation biocatalysts. However, they present different drawbacks (e.g., nutritional supplements requirement, high transcriptional demand of recombinant genes, precise oxygen level, and substrate inhibition) which limit the industrial attractiveness of such conversion process. In this work, we aim to engineer a new bacterial biocatalyst, specific for dairy waste fermentation. We metabolically engineered eight Escherichia coli strains via a new expression plasmid with the pyruvate-to-ethanol conversion genes, and we carried out the selection of the best strain among the candidates, in terms of growth in permeate, lactose consumption and ethanol formation. We finally showed that the selected engineered microbe (W strain) is able to efficiently ferment permeate and concentrated permeate, without nutritional supplements, in pH-controlled bioreactor. In the conditions tested in this work, the selected biocatalyst could complete the fermentation of permeate and concentrated permeate in about 50 and 85 h on average, producing up to 17 and 40 g/l of ethanol, respectively. To our knowledge, this is the first report showing efficient ethanol production from the lactose contained in whey permeate with engineered E. coli. The selected strain is amenable to further metabolic optimization and represents an advance towards efficient biofuel production from industrial waste stream.

  2. Effect of alcohol on skin permeation and metabolism of an ester-type prodrug in Yucatan micropig skin.

    Science.gov (United States)

    Fujii, Makiko; Ohara, Rieko; Matsumi, Azusa; Ohura, Kayoko; Koizumi, Naoya; Imai, Teruko; Watanabe, Yoshiteru

    2017-11-15

    We studied the effect that three alcohols, ethanol (EA), propanol (PA), and isopropanol (IPA), have on the skin permeation of p-hydroxy benzoic acid methyl ester (HBM), a model ester-type prodrug. HBM was applied to Yucatan micropig skin in a saturated phosphate buffered solution with or without 10% alcohol, and HBM and related materials in receptor fluid and skin were determined with HPLC. In the absence of alcohol, p-hydroxy benzoic acid (HBA), a metabolite of HBM, permeated the skin the most. The three alcohols enhanced the penetration of HBM at almost the same extent. The addition of 10% EA or PA to the HBM solution led to trans-esterification into the ethyl ester or propyl ester of HBA, and these esters permeated skin as well as HBA and HBM did. In contrast, the addition of 10% IPA promoted very little trans-esterification. Both hydrolysis and trans-esterification in the skin S9 fraction were inhibited by BNPP, an inhibitor of carboxylesterase (CES). Western blot and native PAGE showed the abundant expression of CES in micropig skin. Both hydrolysis and trans-esterification was simultaneously catalyzed by CES during skin permeation. Our data indicate that the alcohol used in dermal drug preparations should be selected not only for its ability to enhance the solubility and permeation of the drug, but also for the effect on metabolism of the drug in the skin. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. An unheated permeation device for calibrating atmospheric VOC measurements

    Directory of Open Access Journals (Sweden)

    J. Brito

    2011-10-01

    Full Text Available The development of an unpowered permeation device for continuous calibration of in-situ instruments measuring atmospheric volatile organic compounds (VOCs is described. Being lightweight and compact, and containing only negligible amounts of chemicals, the device is especially suited for field use such as on board aircraft. Its speciality is to maintain the permeation process in thermal equilibrium, so that the instantaneous permeation rate can be ascribed to a simple temperature measurement. This equilibrium state is maintained by a combination of three features: (i a thin PTFE membrane as permeation medium which guarantees short stabilization times, (ii a water bath as heat buffer, and (iii a vacuum-panel based insulation, in which features (ii and (iii minimize temperature drifts to ~30 mK h−1 per Kelvin temperature difference to the environment. The respective uncertainty of the permeation rate due to thermal non-equilibrium is kept below 1%. An extensive theory part details the major permeation processes of gases through porous polymers, being Fick's diffusion, Knudsen flow, and viscous flow. Both the measured stabilization time and the measured temperature dependence of the permeation rate independently indicate that the permeation can be described by a viscous flow model, where diffusion of the gas molecules in large pores (having a diameter of >0.05 μm dominates.

  4. Increased albumin permeation in eyes, aorta, and kidney of hypertensive rats fed galactose

    International Nuclear Information System (INIS)

    Tilton, R.G.; LaRose, L.; Chang, K.; Weigel, C.J.; Williamson, J.R.

    1986-01-01

    These experiments were undertaken to determine whether ingestion of galactose increases albumin permeation in the vasculature of hypertensive rats. 50% dextrin (control) or 50% galactose diets were fed to unilaterally nephrectomized, male Sprague-Dawley rats weighing 200 g. Hypertension (systolic pressure >175 mmHg) was induced by weekly IM injections of 25 mg/kg DOCA and 1% saline drinking water; 3 months later 125 I-albumin permeation was assessed in whole eyes, aorta and kidneys. 125 I-albumin permeation was significantly increased in all 3 tissues of hypertensive rats (n = 9) vs controls (n = 9): aorta (3.30 +/- 0.19 (SD) vs 2.87 +/- 0.14), eye (3.15 +/- 0.14 vs 2.59 +/- 0.11), and kidney (6.58 +/- 0.63 vs 3.85 +/- 0.50). Albumin permeation was increased still further in hypertensive rats fed the galactose diet (n = 8): aorta (3.75 +/- 0.38), eye (3.82 +/- 0.17), and kidney (10.74 +/- 3.13). Hypertension +/- galactose feeding had no effect on albumin permeation in lung, skin, or brain. These findings indicate that: (1) hypertension increases albumin permeation in vessels affected by diabetic vascular diseases, and 2) hypertension-induced increases in albumin permeation are increased still further by galactose ingestion, presumably mediated by imbalances in polyol/insitol metabolism (analogous to those induced by diabetes) independent of hyperglycemia and/or insulinopenia

  5. Permeation through graphene ripples

    Science.gov (United States)

    Liang, Tao; He, Guangyu; Wu, Xu; Ren, Jindong; Guo, Hongxuan; Kong, Yuhan; Iwai, Hideo; Fujita, Daisuke; Gao, Hongjun; Guo, Haiming; Liu, Yingchun; Xu, Mingsheng

    2017-06-01

    Real graphene sheets show limited anti-permeation performance deviating from the ideally flat honeycomb carbon lattice that is impermeable to gases. Ripples in graphene are prevalent and they could significantly influence carrier transport. However, little attention has been paid to the role of ripples in the permeation properties of graphene. Here, we report that gases can permeate through graphene ripples at room temperature. The feasibility of gas permeation through graphene ripples is determined by detecting the initial oxidation sites of Cu surface covered with isolated graphene domain. Nudged elastic band (NEB) calculations demonstrate that the oxygen atom permeation occurs via the formation of C-O-C bond, in which process the energy barrier through the rippled graphene lattice is much smaller than that through a flat graphene lattice, rendering permeation through ripples more favorable. Combining with the recent advances in atoms intercalation between graphene and metal substrate for transfer-free and electrically insulated graphene, this discovery provides new perspectives regarding graphene’s limited anti-permeation performance and evokes for rational design of graphene-based encapsulation for barrier and selective gas separation applications through ripple engineering.

  6. In-pile tritium permeation experiment

    International Nuclear Information System (INIS)

    Longhurst, G.R.; Miller, L.G.; Watts, K.D.; Kershner, C.J.; Rogers, M.L.

    1982-01-01

    The experiments in progress are examining various aspects of the permeation of hydrogen isotopes through fusion materials. Of particular importance will be the measurement of permeation due to ion implantation in the presence of a neutron radiation field. Theoretical and early experimental results for these experiments have suggested that sufficient tritium will permeate fusion reactor interior structures that development of a permeation barrier will be needed. (orig.)

  7. In-pile tritium permeation experiment

    Energy Technology Data Exchange (ETDEWEB)

    Longhurst, G.R.; Miller, L.G.; Watts, K.D. (Idaho National Engineering Lab., Idaho Falls (USA)); Kershner, C.J.; Rogers, M.L. (Monsanto Research Corp., Miamisburg, OH (USA). Mound Facility)

    The experiments in progress are examining various aspects of the permeation of hydrogen isotopes through fusion materials. Of particular importance will be the measurement of permeation due to ion implantation in the presence of a neutron radiation field. Theoretical and early experimental results for these experiments have suggested that sufficient tritium will permeate fusion reactor interior structures that development of a permeation barrier will be needed.

  8. Development of Novel Formulations to Enhance in Vivo Transdermal Permeation of Tocopherol

    Directory of Open Access Journals (Sweden)

    Nada Aly H.

    2014-09-01

    Full Text Available Tocopherol represents a big challenge for transdermal permeation owing to its extreme hydrophobicity and large molecular mass. The aim of the present study was to develop alpha-tocopherol (T topical formulations and evaluate their ex vivo and in vivo permeation. Franz diffusion cells were used for ex vivo permeation, and neonatal rats were used for in vivo permeation. Seven gel formulations and 21 liquid formulations were investigated for physical stability, viscosity and permeation of T. Analysis of T was performed by a validated HPLC method using a UV detector. The ex vivo permeation from gel and emulsion formulations was very poor (0.001-0.015 %. Highest permeation was observed from monophasic liquid formulations containing dimethyl sulfoxide (DMSO, tocopheryl polyethylene glycols (TPGs, propylene glycol, ethanol and 9.5 % T. The in vivo results demonstrated higher retention in the epidermis compared to subcutaneous tissues, 1377 and 1.13 μg g-1, respectively. Increasing T concentration from 4.8 to 9.5 % did not increase the amount permeated or % of T retained. It was concluded that simple solutions of T in the presence of DMSO and TPGs were more promising systems for effective transdermal permeation compared to gel, emulsion or oleaginous systems.

  9. LIBRETTO-3: modelling tritium extraction/permeation and evaluation of permeation barriers under irradiation

    International Nuclear Information System (INIS)

    Sedano, L.A.; Fuetterer, M.A.; Viola, R.; Dies, X.

    1996-01-01

    Permeation barriers are required in order to limit the size and cost of the detritiation plants for future fusion reactor blankets of the water-cooled Pb-17Li type. The LIBRETTO irradiations were performed to evaluate the efficiency of permeation barriers under high flux reactor (HFR) conditions. Tritium extraction and permeation characteristics from Pb-17Li under variable temperatures 553-723 K, H 2 doping (0-1 vol%) and purge gas flow rates 20-100 scc/min were tested in LIBRETTO-3. An external TiC coating, an internal (TiC+Al 2 O 3 ), both produced by chemical vapour deposition (CVD), and an internal Al 2 O 3 produced by pack cementation (PC) on AISI 316L steel were tested as permeation barriers. The release mechanisms, experimental uncertainties and method for permeation barriers qualification are presented. As a result permeation reduction factors (PRF) at 0.1 dpa of 17 and 34 were obtained for the CVD-Al 2 O 3 at 498 K and for the PC-Al 2 O 3 at 508 K, respectively. These values were confirmed by a residence time analysis and are higher than in a preliminary analysis. (orig.)

  10. Modeling benzene permeation through drinking water high density polyethylene (HDPE) pipes.

    Science.gov (United States)

    Mao, Feng; Ong, Say Kee; Gaunt, James A

    2015-09-01

    Organic compounds such as benzene, toluene, ethyl benzene and o-, m-, and p-xylene from contaminated soil and groundwater may permeate through thermoplastic pipes which are used for the conveyance of drinking water in water distribution systems. In this study, permeation parameters of benzene in 25 mm (1 inch) standard inside dimension ratio (SIDR) 9 high density polyethylene (HDPE) pipes were estimated by fitting the measured data to a permeation model based on a combination of equilibrium partitioning and Fick's diffusion. For bulk concentrations between 6.0 and 67.5 mg/L in soil pore water, the concentration-dependent diffusion coefficients of benzene were found to range from 2.0×10(-9) to 2.8×10(-9) cm2/s while the solubility coefficient was determined to be 23.7. The simulated permeation curves of benzene for SIDR 9 and SIDR 7 series of HDPE pipes indicated that small diameter pipes were more vulnerable to permeation of benzene than large diameter pipes, and the breakthrough of benzene into the HDPE pipe was retarded and the corresponding permeation flux decreased with an increase of the pipe thickness. HDPE pipes exposed to an instantaneous plume exhibited distinguishable permeation characteristics from those exposed to a continuous source with a constant input. The properties of aquifer such as dispersion coefficients (DL) also influenced the permeation behavior of benzene through HDPE pipes.

  11. Deuterium permeation through Flibe facing materials

    International Nuclear Information System (INIS)

    Fukada, S.; Anderl, R.A.; Smolik, G.R.

    2004-01-01

    Experiment of deuterium permeation through Ni facing with purified Flibe is being carried out under the Japan-US joint research project (JUPITER-II). The experiment has been proceeding in the following phases; (i) fabrication and assembly of a dual-probe permeation apparatus, (ii) a single-probe Ni/D 2 , permeation experiment without Flibe, (iii) a dual-probe Ar/Ni/D 2 permeation experiment without Flibe, (iv) Flibe chemical purification by HF/H 2 gas bubbling, (v) physical purification by Flibe transport through a porous Ni filter, (vi) Ar/Ni/Flibe/Ni/D 2 permeation experiment using the dual Ni probe, and (vii) Ar/Ni/Flibe/Ni/HT permeation experiment. The present paper describe results until the Ar/Ni/Flibe/Ni/D 2 permeation experiment in detail. (author)

  12. On Diffusion and Permeation

    KAUST Repository

    Peppin, Stephen S. L.

    2009-01-01

    concentrations they form a nearly rigid porous glass through which the fluid permeates. The theoretically determined pressure drop is nonlinear in the diffusion regime and linear in the permeation regime, in quantitative agreement with experimental measurements

  13. Water permeation through anion exchange membranes

    Science.gov (United States)

    Luo, Xiaoyan; Wright, Andrew; Weissbach, Thomas; Holdcroft, Steven

    2018-01-01

    An understanding of water permeation through solid polymer electrolyte (SPE) membranes is crucial to offset the unbalanced water activity within SPE fuel cells. We examine water permeation through an emerging class of anion exchange membranes, hexamethyl-p-terphenyl poly (dimethylbenzimidazolium) (HMT-PMBI), and compare it against series of membrane thickness for a commercial anion exchange membrane (AEM), Fumapem® FAA-3, and a series of proton exchange membranes, Nafion®. The HMT-PMBI membrane is found to possess higher water permeabilities than Fumapem® FAA-3 and comparable permeability than Nafion (H+). By measuring water permeation through membranes of different thicknesses, we are able to decouple, for the first time, internal and interfacial water permeation resistances through anion exchange membranes. Permeation resistances on liquid/membrane interface is found to be negligible compared to that for vapor/membrane for both series of AEMs. Correspondingly, the resistance of liquid water permeation is found to be one order of magnitude smaller compared to that of vapor water permeation. HMT-PMBI possesses larger effective internal water permeation coefficient than both Fumapem® FAA-3 and Nafion® membranes (60 and 18% larger, respectively). In contrast, the effective interfacial permeation coefficient of HMT-PMBI is found to be similar to Fumapem® (±5%) but smaller than Nafion®(H+) (by 14%).

  14. Tritium permeation through iron

    International Nuclear Information System (INIS)

    Hagi, Hideki; Hayashi, Yasunori

    1989-01-01

    An experimental method for measuring diffusion coefficients and permeation rates of tritium in metals around room temperature has been established, and their values in iron have been obtained by using the method. Permeation rates of tritium and hydrogen through iron were measured by the electrochemical method in which a tritiated aqueous solution was used as a cathodic electrolyte. Tritium and hydrogen were introduced from one side of a membrane specimen by cathodic polarization, while at the other side of the specimen the permeating tritium and hydrogen were extracted by potentiostatical ionization. The amount of permeated hydrogen was obtained by integrating the anodic current, and that of tritium was determined by measuring the radioactivity of the electrolyte sampled from the extraction side. Diffusion coefficients of tritium (D T ) and hydrogen (D H ) were determined from the time lag of tritium and hydrogen permeation. D T =9x10 -10 m 2 /s and D H =4x10 -9 m 2 /s at 286 K for annealed iron specimens. These values of D T and D H were compared with the previous data of the diffusion coefficients of hydrogen and deuterium, and the isotope effect in diffusion was discussed. (orig.)

  15. Tritium permeation in fusion reactors: INTOR

    International Nuclear Information System (INIS)

    Baskes, M.I.; Bauer, W.; Kerst, R.A.; Swansiger, W.A.; Wilson, K.L.

    1981-12-01

    Tritium permeation through the first wall of advanced fusion reactors is examined. A fraction of the D-T which bombards the first wall as charge exchange neutral particles will permeate through the first wall and enter the coolant. Calculations of the steady state permeation rate for the US INTOR Tokamak design result in values of less than or equal to 0.002 grams of tritium per day under the most favorable conditions. For unfavorable surface conditions the rate is greater than or equal to 0.1 g/day. The magnitude of these permeation rates is critically dependent on the temperatures and surface conditions of the wall. The introduction of permeation barriers at the wall-coolant interface can significantly reduce permeation rates and hence may be desirable for reactor applications

  16. Permeation behavior of deuterium implanted into beryllium

    Energy Technology Data Exchange (ETDEWEB)

    Nakamura, Hirofumi; Hayashi, Takumi; O' hira, Shigeru; Nishi, Masataka [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

    2001-09-01

    Study on Implantation Driven Permeation (IDP) behavior of deuterium through pure beryllium was investigated as a part of the research to predict the tritium permeation through the first wall components ITER (International Thermonuclear Experimental Reactor). The permeation experiments were carried out with two beryllium specimens, one was an unannealed specimen and the other was that annealed at 1173 K. The permeation flux was measured as a function of specimen temperature and incident ion flux. Surface analysis of specimen was also carried out after the permeation experiment. Permeation was observed only with the annealed specimen and no significant permeation was observed with unannealed specimen under the present experimental condition (maximum temperature: 685 K, detection limit: 1x10{sup 13} D atoms/m{sup 2}s). It could be attributed that the intrinsic lattice defects, which act as diffusion preventing site, decreased with the specimen annealing. Based on the result of steady and transient permeation behavior and surface analysis, it was estimated that the deuterium permeation implanted into annealed beryllium was controlled by surface recombination due to the oxide layer on the surface of the permeated side. (author)

  17. Flibe-D2 Permeation Experiment and Analysis

    International Nuclear Information System (INIS)

    Fukada, S.; Anderl, R.A.; Pawelko, R.J.; Smolik, G.R.; Schuetz, S.T.; O'Brien, J.E.; Nishimura, H.; Hatano, Y.; Terai, T.; Petti, D.A.; Sze, D.-K.; Tanaka, S.

    2003-01-01

    Experiment of D 2 permeation through Ni facing with purified Flibe is being carried out under the Japan-US joint research project (JUPITER-II). The experiment is proceeding in the following phases; (i) fabrication and assembly of a dual-probe permeation apparatus, (ii) a single-probe Ni/D 2 permeation experiment without Flibe, (iii) a dual-probe Ni/D 2 permeation experiment without Flibe, (iv) Flibe chemical purification by HF/H 2 gas bubbling, (v) physical purification by Flibe transport through a porous Ni filter, (vi) Ni/Flibe/D 2 permeation experiment, and (vii) Ni/Flibe/HT permeation experiment. The present paper describes results of the single and dual Ni/D 2 permeation experiments in detail

  18. Ultrathin graphene-based membrane with precise molecular sieving and ultrafast solvent permeation

    Science.gov (United States)

    Yang, Q.; Su, Y.; Chi, C.; Cherian, C. T.; Huang, K.; Kravets, V. G.; Wang, F. C.; Zhang, J. C.; Pratt, A.; Grigorenko, A. N.; Guinea, F.; Geim, A. K.; Nair, R. R.

    2017-12-01

    Graphene oxide (GO) membranes continue to attract intense interest due to their unique molecular sieving properties combined with fast permeation. However, their use is limited to aqueous solutions because GO membranes appear impermeable to organic solvents, a phenomenon not yet fully understood. Here, we report efficient and fast filtration of organic solutions through GO laminates containing smooth two-dimensional (2D) capillaries made from large (10-20 μm) flakes. Without modification of sieving characteristics, these membranes can be made exceptionally thin, down to ~10 nm, which translates into fast water and organic solvent permeation. We attribute organic solvent permeation and sieving properties to randomly distributed pinholes interconnected by short graphene channels with a width of 1 nm. With increasing membrane thickness, organic solvent permeation rates decay exponentially but water continues to permeate quickly, in agreement with previous reports. The potential of ultrathin GO laminates for organic solvent nanofiltration is demonstrated by showing >99.9% rejection of small molecular weight organic dyes dissolved in methanol. Our work significantly expands possibilities for the use of GO membranes in purification and filtration technologies.

  19. On Diffusion and Permeation

    KAUST Repository

    Peppin, Stephen S. L.

    2009-01-01

    Diffusion and permeation are discussed within the context of irreversible thermodynamics. A new expression for the generalized Stokes-Einstein equation is obtained which links the permeability to the diffusivity of a two-component solution and contains the poroelastic Biot-Willis coefficient. The theory is illustrated by predicting the concentration and pressure profiles during the filtration of a protein solution. At low concentrations the proteins diffuse independently while at higher concentrations they form a nearly rigid porous glass through which the fluid permeates. The theoretically determined pressure drop is nonlinear in the diffusion regime and linear in the permeation regime, in quantitative agreement with experimental measurements. © 2009 Walter de Gruyter, Berlin, New York.

  20. Stability of tritium permeation prevention barrier with TiC and TiN + TiC coating

    International Nuclear Information System (INIS)

    Shan Changqi; Chen Qingwang; Dai Shaoxia; Jiang Weisheng

    1999-01-01

    The stability of tritium permeation prevention barrier of 316L stainless steel with coating TiC and TiN + TiC under the conditions of very large thermal gradient, thermal cycling and plasma irradiation is researched. The research includes two aspects: one is the study on the stability resisting H + plasma irradiation; another is on the ability of two coating materials when they are used in long term under the condition of very large thermal gradient and cycling. The results show that TiC and TiN + TiC composite coating materials, after chemical heat treatment and forming tritium permeation prevention barrier, can resist H + ion irradiation, and also can resist very large thermal gradient and thermal cycling. The long time experiments show that tritium permeation prevention barrier of those coating materials is stable when they are used in long term

  1. In vitro metabolism and permeation studies in rat jejunum

    DEFF Research Database (Denmark)

    Gammelgaard, Bente; Jensen, K; Steffansen, B

    1999-01-01

    The purpose of these studies was to compare the in vitro absorption of two inorganic chromium(III) compounds: chromium chloride and chromium nitrate, with organic chromium(III)-picolinate; and to investigate if any in vitro metabolism of chromium(VI) takes place. The in vitro metabolism studies...

  2. Tritium permeation barriers in contact with liquid lithium-lead eutectic (Pb-17Li)

    International Nuclear Information System (INIS)

    Forcey, K.S.; Perujo, A.

    1995-01-01

    The permeation of deuterium through coated stainless steel tubes containing liquid lithium-lead eutectic (Pb-17Li) has been studied and compared to measurements through tubes without the lithium compound. The measurements form part of an investigation into the effect of a potential tritium breeder material on permeation barriers for fusion reactors. The coatings studied were CVD TiC and Al 2 O 3 and a pack aluminised layer. Without the lithium-lead, the CVD coatings reduced the permeation rate up to 1 order of magnitude, and the aluminised layer up to 2 orders of magnitude. A CVD layer was unaffected by Pb-17Li whilst in the case of the aluminised tube, the lithium-lead completely removed the permeation barrier, presumably by attacking the surface oxide. Furthermore, the aluminised sample presented a large number of cracks and poor adheren ce to the substrate. ((orig.))

  3. Evaluation of whey, milk, and delactosed permeates as salt substitutes.

    Science.gov (United States)

    Smith, S T; Metzger, L; Drake, M A

    2016-11-01

    Whey and milk permeates are by-products of high-protein dairy powder manufacture. Previous work has shown that these permeates contribute to salty taste without contributing significantly to sodium content. The objective of this study was to explore the sensory characteristics and compositional analysis of permeates from different milk and whey streams and a low-sodium product application made from them. Skim milk, Cheddar, cottage, and Mozzarella cheese whey permeates were manufactured in triplicate, and delactosed whey permeate was obtained in triplicate. Composition (protein, fat, solids, minerals) was conducted on permeates. Organic acid composition was determined using HPLC. Volatile compounds were extracted from permeates by solid phase microextraction with gas chromatography-mass spectrometry. A trained sensory panel documented sensory attributes of permeates and cream of broccoli soups with and without salt or permeates followed by consumer acceptance testing (n=105) on the soups. Cottage cheese whey permeate contained a higher lactic acid content than other permeates, which has been shown to contribute to a higher salty taste. Cottage cheese whey permeate also contained potato or brothy and caramel flavors and sour and salty tastes, whereas delactosed whey permeate had high intensities of cardboard and beefy or brothy flavors and salty taste. Milk, Cheddar, and Mozzarella cheese whey permeates were characterized by sweet taste and cooked milky flavor. Permeates with higher cardboard flavor had higher levels of aldehydes. All permeates contributed to salty taste and to salty taste perception in soups; although the control soup with added salt was perceived as saltier and was preferred by consumers over permeate soups. Soup with permeate from cottage cheese was the least liked of all soups, likely due to its sour taste. All other permeate soups scored at parity for liking. These results demonstrate the potential for milk, whey, and delactosed permeates from

  4. Contaminant Permeation in the Ionomer-Membrane Water Processor (IWP) System

    Science.gov (United States)

    Kelsey, Laura K.; Finger, Barry W.; Pasadilla, Patrick; Perry, Jay

    2016-01-01

    The Ionomer-membrane Water Processor (IWP) is a patented membrane-distillation based urine brine water recovery system. The unique properties of the IWP membrane pair limit contaminant permeation from the brine to the recovered water and purge gas. A paper study was conducted to predict volatile trace contaminant permeation in the IWP system. Testing of a large-scale IWP Engineering Development Unit (EDU) with urine brine pretreated with the International Space Station (ISS) pretreatment formulation was then conducted to collect air and water samples for quality analysis. Distillate water quality and purge air GC-MS results are presented and compared to predictions, along with implications for the IWP brine processing system.

  5. Counter-diffusion and -permeation of deuterium and hydrogen through metals

    Energy Technology Data Exchange (ETDEWEB)

    Kizu, Kaname; Tanabe,; Tetsuo, [Nagoya Univ. (Japan)

    1998-03-01

    The first experiments for counter-diffusion and -permeation of deuterium and hydrogen through palladium were performed. Deuterium permeation rates against D{sub 2} pressure were measured under the condition where hydrogen permeated to opposite direction by supplying H{sub 2} gas at the permeated side of D{sub 2}. It was found that not a small amount of deuterium was clearly permeated even if the deuterium pressure was much smaller than the hydrogen pressure. Deuterium permeation rate was gradually reduced by increasing the counter H permeation. The deuterium permeation rate under the counter H permeation is well represented by a simple model in which the ratio of the deuterium permeation rates with and without the counter H permeation was proportional to the fractional concentration of deuterium in the bulk. As increasing the hydrogen counter flow, however, the deuterium permeation rate deviates from the model. This means that adsorption (absorption) of D{sub 2} from gas phase is inhibited and surface recombination of deuterium is blocked by hydrogen. (author)

  6. Permeation of Comite through protective gloves.

    Science.gov (United States)

    Zainal, Hanaa; Que Hee, Shane S

    2006-09-01

    The goal of the study was to assess how protective disposable (Safeskin) and chemical protective (Sol-Vex) nitrile gloves were against Comite emulsifiable concentrate formulation containing propargite (PROP) as active pesticidal ingredient, because there were no explicit recommendations for the gloves that should be worn for hand protection. The glove material was exposed in ASTM-type I-PTC-600 permeation cells at 30.0+/-0.5 degrees C, and gas chromatography-mass spectrometry used for PROP analysis. Aqueous solutions of Comite at 40.4 mg/mL permeated both Safeskin and Sol-Vex nitrile by 8h. Safeskin showed a mean PROP mass permeated of 176+/-27 microg after 8h compared with a mean mass permeated for Sol-Vex of 3.17+/-4.08 microg. Thus, Sol-Vex was about 56 times more protective than Safeskin for an 8-h exposure. However, the kinetics of the permeation revealed that Safeskin can be worn for at least 200 min before disposal. When undiluted Comite challenged both types of nitrile, much faster permeation was observed. Safeskin gloves showed two steady state periods. The first had lag times (t(l)) values of about 1h, although normalized breakthrough times (t(b)) were gloves exposed continuously to undiluted Comite permeated above the normalized breakthrough threshold beyond 2.7h. A risk assessment revealed that the PROP skin permeation rate of 7.1 ng cm(-2)h(-1) was much slower than the first steady state Safeskin glove P(s) of 62,000 ng cm(-2)h(-1). Infrared analysis showed that the glove surfaces were not degraded by the Comite challenge. The chemically protective Sol-Vex gloves protected adequately against undiluted formulation for about 2.7h, whereas they provided protection for nearly 8h when the formulation was diluted with water to the highest concentration for field application. In contrast, the disposable Safeskin gloves did not protect at all for the undiluted formulation, but did for 200 min when the formulation was diluted with water to the highest

  7. Tritium permeation through helium-heated steam generators of ceramic breeder blankets for DEMO

    International Nuclear Information System (INIS)

    Fuetterer, M.A.; Raepsaet, X.; Proust, E.

    1994-01-01

    The specifications of permeation barriers, tritium recovery process maintaining a very low tritium activity in the coolant, and control of the coolant chemistry, required the evaluation of the tritium losses through the steam generators and include the definition of its operating conditions by thermodynamic cycle calculations and its thermal-hydraulic design. For both tasks specific computer tools were developed. The obtained geometry, surface area, and temperature profiles along the heat exchanger tubes were then used to estimate the daily tritium permeation into the steam cycle. Steam oxidized Incoloy 800 austenitic stainless steel was identified as the best suited existing material; in nominal steady-state operation, the tritium escape into the steam cycle could be restricted to less than 10 Ci/d. Tritium permeation during temperature and pressure transients in the steam generator (destruction and possible self-healing of the permeation barrier) is identified to bear a large tritium release potential. Solutions are proposed. (from authors). 4 figs., 1 tab

  8. Tritium permeation through helium-heated steam generators of ceramic breeder blankets for DEMO

    Energy Technology Data Exchange (ETDEWEB)

    Fuetterer, M A; Raepsaet, X; Proust, E

    1994-12-31

    The specifications of permeation barriers, tritium recovery process maintaining a very low tritium activity in the coolant, and control of the coolant chemistry, required the evaluation of the tritium losses through the steam generators and include the definition of its operating conditions by thermodynamic cycle calculations and its thermal-hydraulic design. For both tasks specific computer tools were developed. The obtained geometry, surface area, and temperature profiles along the heat exchanger tubes were then used to estimate the daily tritium permeation into the steam cycle. Steam oxidized Incoloy 800 austenitic stainless steel was identified as the best suited existing material; in nominal steady-state operation, the tritium escape into the steam cycle could be restricted to less than 10 Ci/d. Tritium permeation during temperature and pressure transients in the steam generator (destruction and possible self-healing of the permeation barrier) is identified to bear a large tritium release potential. Solutions are proposed. (from authors). 4 figs., 1 tab.

  9. In-site coatings to reduce H and Tr permeation

    International Nuclear Information System (INIS)

    Stoever, D.; Buchkremer, H.P.; Hecker, R.; Jonas, H.; Schaefer, J.; Zink, U.; Forsyth, N.; Thiele, W.

    1982-01-01

    The main goal of this project is the development of protective coatings to reduce or prevent Tr and H permeation through the heat exchanger walls of HTR components. The tasks of the project are: Measurement of the permeation inhibition efficiency of oxidic coatings on the high-temperature- resistant heat exchanger walls; establishing the parameters influencing permeation by variation of the process gas and steam parameters, temperature and mechanical stress; characterisation of coatings and correlation of coating characteristics with permeation measurements; investigation of permeation and corrosion mechanisms; quantitative description of H and Tr permeation by means of mathematical/physical models. (orig./IHOE) [de

  10. Implantation driven permeation behavior of deuterium through pure tungsten

    Energy Technology Data Exchange (ETDEWEB)

    Nakamura, Hirofumi E-mail: nakamura@tpl.tokai.jaeri.go.jp; Hayashi, Takumi; Nishi, Masataka; Arita, Makoto; Okuno, Kenji

    2001-09-01

    Implantation driven permeation behavior of deuterium through pure tungsten has been investigated to estimate the amount of tritium permeation through its barrier in a thermo-nuclear fusion device. The permeation experiments were performed on pure tungsten foil of 25 {mu}m thickness under conditions of incident flux of 1.9x10{sup 18}-1.1x10{sup 19} D{sup +}/m{sup 2}s, incident ion energy of 200-2000 eV, and specimen temperature of 512-660 K. As a result of this steady-state permeation experiment, the rate-determining process of deuterium permeation was found to be controlled by diffusion at both implanted and permeated sides. On the other hand, transient permeation was strongly affected by trap effect in the specimen. Simulation analysis using TMAP code on transient permeation behavior indicates the existence of a trap site with a trap energy of nearly 1eV and with a trap density of over several ten's ppm in tungsten.

  11. Implantation driven permeation behavior of deuterium through pure tungsten

    International Nuclear Information System (INIS)

    Nakamura, Hirofumi; Hayashi, Takumi; Nishi, Masataka; Arita, Makoto; Okuno, Kenji

    2001-01-01

    Implantation driven permeation behavior of deuterium through pure tungsten has been investigated to estimate the amount of tritium permeation through its barrier in a thermo-nuclear fusion device. The permeation experiments were performed on pure tungsten foil of 25 μm thickness under conditions of incident flux of 1.9x10 18 -1.1x10 19 D + /m 2 s, incident ion energy of 200-2000 eV, and specimen temperature of 512-660 K. As a result of this steady-state permeation experiment, the rate-determining process of deuterium permeation was found to be controlled by diffusion at both implanted and permeated sides. On the other hand, transient permeation was strongly affected by trap effect in the specimen. Simulation analysis using TMAP code on transient permeation behavior indicates the existence of a trap site with a trap energy of nearly 1eV and with a trap density of over several ten's ppm in tungsten

  12. Permeation of hydrogen through metal membranes

    International Nuclear Information System (INIS)

    Wienhold, P.; Rota, E.; Waelbroeck, F.; Winter, J.; Banno, Tatsuya.

    1986-08-01

    Experiments show that the permeant flux of hydrogen through a metal membrane at low driving pressures ( r is introduced into the model as a new material constant and the rate equations are given. After the description of the wall pump effect, a variety of different limiting cases are discussed for a symmetrical permeation membrane. This is modified to the asymmetric case and to the influence of particle implantation. The permeation number W turns out to be a dimensionless quantity which characterizes the permeation range and predicts the permeant flux in steady state. (orig.)

  13. Permeation Tests on Polypropylene Fiber Materials

    Science.gov (United States)

    2018-03-16

    Permeation Tests on Polypropylene Fiber Materials Brandy J. White Martin H. Moore Brian J. Melde Laboratory for the Study of Molecular Interfacial...ABSTRACT Permeation Tests on Polypropylene Fiber Materials Brandy J. White, Martin H. Moore, Brian J. Melde Center for Bio/Molecular Science

  14. In situ measurement of tritium permeation through stainless steel

    Science.gov (United States)

    Luscher, Walter G.; Senor, David J.; Clayton, Kevin K.; Longhurst, Glen R.

    2013-06-01

    The TMIST-2 irradiation experiment was conducted in the Advanced Test Reactor at Idaho National Laboratory to evaluate tritium permeation through Type 316 stainless steel (316 SS). The interior of a 316 SS seamless tube specimen was exposed to a 4He carrier gas mixed with a specified quantity of tritium (T2) to yield partial pressures of 0.1, 5, and 50 Pa at 292 °C and 330 °C. In situ tritium permeation measurements were made by passing a He-Ne sweep gas over the outer surface of the specimen to carry the permeated tritium to a bubbler column for liquid scintillation counting. Results from in situ permeation measurements were compared with predictions based on an ex-reactor permeation correlation in the literature. In situ permeation data were also used to derive an in-reactor permeation correlation as a function of temperature and pressure over the ranges considered in this study. In addition, the triton recoil contribution to tritium permeation, which results from the transmutation of 3He to T, was also evaluated by introducing a 4He carrier gas mixed with 3He at a partial pressure of 1013 Pa at 330 °C. Less than 3% of the tritium resulting from 3He transmutation contributed to tritium permeation.

  15. Extent of cutaneous metabolism during percutaneous absorption of xenobiotics.

    Science.gov (United States)

    Bronaugh, R L; Stewart, R F; Storm, J E

    1989-07-01

    In vitro percutaneous absorption studies generally do not determine whether biotransformation occurs during passage of a substance through the skin. Since it has recently been demonstrated that several chemicals are metabolized during skin permeation, we investigated the metabolism of five additional compounds (14C-labeled) after application to fuzzy rat skin: caffeine, p,p'-DDT, butylated hydroxytoluene (BHT), salicylic acid, and acetyl ethyl tetramethyltetralin (AETT). The viability of skin was maintained with a tissue culture medium. Radioactivity of each substrate and any metabolites in skin and receptor fluid was measured so that the absorption and metabolism of water-insoluble compounds would be accurately determined. Percutaneous absorption ranged from a low of 13% of the applied dose for BHT to a high of 49% for DDT. BHT was metabolized in skin to 4-hydroxy-BHT and an unknown metabolite. Of the absorbed radioisotope, 6.6% was isolated in biotransformed products found mainly in the receptor fluid. AETT was also metabolized during absorption, with 1.9% of the absorbed radioisotope found in two unknown peaks. Caffeine, DDT, and salicylic acid were not metabolized during skin permeation. Skin and liver microsomal metabolism was measured for all compounds except DDT. Metabolism in skin was observed only for the compounds also biotransformed in the diffusion cell; BHT and AETT were metabolized at 113 and 2.5 pmol/min/mg protein, respectively. In this study, as in others, skin metabolism was substantially less than the corresponding metabolism in liver. Therefore, a low rate of liver metabolism such as that found for caffeine, salicylic acid, and DDT might often be predictive of the absence of measurable metabolism during skin permeation. It seems likely that for many compounds, the biotransformations in skin will be small in terms of the percentage of absorbed material that is metabolized. Nevertheless, with potent compounds, even small quantities of a metabolite

  16. In situ measurement of tritium permeation through stainless steel

    Energy Technology Data Exchange (ETDEWEB)

    Luscher, Walter G., E-mail: walter.luscher@pnnl.gov [Pacific Northwest National Laboratory, 902 Battelle Blvd, Richland, WA 99352 (United States); Senor, David J., E-mail: david.senor@pnnl.gov [Pacific Northwest National Laboratory, 902 Battelle Blvd, Richland, WA 99352 (United States); Clayton, Kevin K., E-mail: kevin.clayton@inl.gov [Idaho National Laboratory, 2525 Fremont Ave., Idaho Falls, ID 83415 (United States); Longhurst, Glen R., E-mail: glenlonghurst@suu.edu [Idaho National Laboratory, 2525 Fremont Ave., Idaho Falls, ID 83415 (United States)

    2013-06-15

    Highlights: ► In situ tritium permeation measurements collected over broad pressure range. ► Test conditions relevant to 316 SS in commercial light water reactors. ► Comparisons between in- and ex-reactor measurements provided. ► Correlation between tritium permeation, temperature, and pressure developed. -- Abstract: The TMIST-2 irradiation experiment was conducted in the Advanced Test Reactor at Idaho National Laboratory to evaluate tritium permeation through Type 316 stainless steel (316 SS). The interior of a 316 SS seamless tube specimen was exposed to a {sup 4}He carrier gas mixed with a specified quantity of tritium (T{sub 2}) to yield partial pressures of 0.1, 5, and 50 Pa at 292 °C and 330 °C. In situ tritium permeation measurements were made by passing a He–Ne sweep gas over the outer surface of the specimen to carry the permeated tritium to a bubbler column for liquid scintillation counting. Results from in situ permeation measurements were compared with predictions based on an ex-reactor permeation correlation in the literature. In situ permeation data were also used to derive an in-reactor permeation correlation as a function of temperature and pressure over the ranges considered in this study. In addition, the triton recoil contribution to tritium permeation, which results from the transmutation of {sup 3}He to T, was also evaluated by introducing a {sup 4}He carrier gas mixed with {sup 3}He at a partial pressure of 1013 Pa at 330 °C. Less than 3% of the tritium resulting from {sup 3}He transmutation contributed to tritium permeation.

  17. Tritium permeation barriers for fusion technology

    International Nuclear Information System (INIS)

    Perujo, A.; Forcey, K.

    1994-01-01

    An important issue concerning the safety, feasibility and fueling (i.e., tritium breeding ratio and recovery from the breeding blanket) of a fusion reactor is the possible tritium leakages through the structural materials and in particular through those operating at high temperatures. The control of tritium permeation could be a critical factor in determining the viability of a future fusion power reactor. The formation of tritium permeation barriers to prevent the loss of tritium to the coolant by diffusion though the structural material seems to be the most practical method to minimize such losses. Many authors have discussed the formation of permeation barriers to reduce the leakage of hydrogen isotopes through proposed first wall and structural materials. In general, there are two routes for the formation of such a barrier, namely: the growth of oxide layers (e.g., Cr 2 O 3 , Al 2 O 3 , etc.) or the application of surface coatings. Non-metals are the most promising materials from the point of view of the formation of permeation barriers. Oxides such as Al 2 O 3 or Cr 2 O 3 or carbides such as SiC or TiC have been proposed. Amongst the metals only tungsten or gold are sufficiently less permeable than steel to warrant investigation as candidate materials for permeation barriers. It is of course possible to grow oxide layers on steel directly by heating in the atmosphere or under a variety of conditions (first route above). The direct oxidizing is normally done in an environment of open-quotes wet hydrogenclose quotes to promote the growth of chromia on, for example, nickel steels or ternary oxides on 316L to prevent corrosion. The application of surface layers (second route above), offers a greater range of materials for the formation of permeation barriers. In addition to reducing permeation, such layers should be adhesive, resistant to attack by corrosive breeder materials and should not crack during thermal cycling

  18. Transcriptome and membrane fatty acid analyses reveal different strategies for responding to permeating and non-permeating solutes in the bacterium Sphingomonas wittichii

    Directory of Open Access Journals (Sweden)

    Johnson David R

    2011-11-01

    Full Text Available Abstract Background Sphingomonas wittichii strain RW1 can completely oxidize dibenzo-p-dioxins and dibenzofurans, which are persistent contaminants of soils and sediments. For successful application in soil bioremediation systems, strain RW1 must cope with fluctuations in water availability, or water potential. Thus far, however, little is known about the adaptive strategies used by Sphingomonas bacteria to respond to changes in water potential. To improve our understanding, strain RW1 was perturbed with either the cell-permeating solute sodium chloride or the non-permeating solute polyethylene glycol with a molecular weight of 8000 (PEG8000. These solutes are assumed to simulate the solute and matric components of the total water potential, respectively. The responses to these perturbations were then assessed and compared using a combination of growth assays, transcriptome profiling, and membrane fatty acid analyses. Results Under conditions producing a similar decrease in water potential but without effect on growth rate, there was only a limited shared response to perturbation with sodium chloride or PEG8000. This shared response included the increased expression of genes involved with trehalose and exopolysaccharide biosynthesis and the reduced expression of genes involved with flagella biosynthesis. Mostly, the responses to perturbation with sodium chloride or PEG8000 were very different. Only sodium chloride triggered the increased expression of two ECF-type RNA polymerase sigma factors and the differential expression of many genes involved with outer membrane and amino acid metabolism. In contrast, only PEG8000 triggered the increased expression of a heat shock-type RNA polymerase sigma factor along with many genes involved with protein turnover and repair. Membrane fatty acid analyses further corroborated these differences. The degree of saturation of membrane fatty acids increased after perturbation with sodium chloride but had the

  19. Hydrogen permeation properties of plasma-sprayed tungsten

    International Nuclear Information System (INIS)

    Anderl, R.A.; Pawelko, R.J.; Hankins, M.R.; Longhurst, G.R.; Neiser, R.A.

    1994-01-01

    Tungsten has been proposed as a plasma-facing component material for advanced fusion facilities. This paper reports on laboratory-scale studies that were done to assess the hydrogen permeation properties of plasma-sprayed tungsten for such applications. The work entailed deuterium permeation measurements for plasma-sprayed (PS) tungsten coatings, sputter-deposited (SP) tungsten coatings, and steel substrate material using a mass-analyzed, 3 keV D + 3 ion beam with fluxes of similar 6.5x10 19 D/m 2 s. Extensive characterization analyses for the plasma-sprayed tungsten coatings were made using Auger spectrometry and scanning electron microscopy (SEM). Observed permeation rates through composite PS-tungsten/steel specimens were several orders of magnitude below the permeation levels observed for SP-tungsten/steel composite specimens and pure steel specimens. Characterization analyses indicated that the plasma-sprayed tungsten coating had a nonhomogeneous microstructure that consisted of splats with columnar solidification, partially-melted particles with grain boundaries, and void regions. Reduced permeation levels can be attributed to the complex microstructure and a substantial surface-connected porosity. ((orig.))

  20. Hydrogen permeation properties of plasma-sprayed tungsten

    Energy Technology Data Exchange (ETDEWEB)

    Anderl, R.A. (Idaho National Engineering Lab., EG and G Idaho Inc., Idaho Falls, ID (United States)); Pawelko, R.J. (Idaho National Engineering Lab., EG and G Idaho Inc., Idaho Falls, ID (United States)); Hankins, M.R. (Idaho National Engineering Lab., EG and G Idaho Inc., Idaho Falls, ID (United States)); Longhurst, G.R. (Idaho National Engineering Lab., EG and G Idaho Inc., Idaho Falls, ID (United States)); Neiser, R.A. (Sandia National Laboratories, Albuquerque, NM 87185 (United States))

    1994-09-01

    Tungsten has been proposed as a plasma-facing component material for advanced fusion facilities. This paper reports on laboratory-scale studies that were done to assess the hydrogen permeation properties of plasma-sprayed tungsten for such applications. The work entailed deuterium permeation measurements for plasma-sprayed (PS) tungsten coatings, sputter-deposited (SP) tungsten coatings, and steel substrate material using a mass-analyzed, 3 keV D[sup +][sub 3] ion beam with fluxes of similar 6.5x10[sup 19] D/m[sup 2] s. Extensive characterization analyses for the plasma-sprayed tungsten coatings were made using Auger spectrometry and scanning electron microscopy (SEM). Observed permeation rates through composite PS-tungsten/steel specimens were several orders of magnitude below the permeation levels observed for SP-tungsten/steel composite specimens and pure steel specimens. Characterization analyses indicated that the plasma-sprayed tungsten coating had a nonhomogeneous microstructure that consisted of splats with columnar solidification, partially-melted particles with grain boundaries, and void regions. Reduced permeation levels can be attributed to the complex microstructure and a substantial surface-connected porosity. ((orig.))

  1. Tritium permeation characterization of Al{sub 2}O{sub 3}/FeAl coatings as tritium permeation barriers on 321 type stainless steel containers

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Feilong; Xiang, Xin; Lu, Guangda; Zhang, Guikai, E-mail: zhangguikai@caep.cn; Tang, Tao; Shi, Yan; Wang, Xiaolin

    2016-09-15

    Accurate tritium transport properties of prospective tritium permeation barriers (TPBs) are essential to tritium systems in fusion reactors. By passing a temperature and rate-controlled sweeping gas over specimen surfaces to carry the permeated tritium to an ion chamber, the gas-driven permeation of tritium has been performed on 321 type stainless steel containers with Al{sub 2}O{sub 3}/FeAl barriers, to determine the T-permeation resistant performance and mechanism of the barrier. The tritium permeability of the Al{sub 2}O{sub 3}/FeAl coated container was reduced by 3 orders of magnitude at 500–700 °C by contrast with that of the bare one, which meets the requirement of the tritium permeation reduction factor (PRF) of TPBs for tritium operating components in the CN-HCCB TBM. The Al{sub 2}O{sub 3}/FeAl barrier resists the tritium permeation by the diffusion in the bulk substrate at a limited number of defect sites with an effective area and thickness, suggesting that the TPB quality is a very important factor for efficient T-permeation resistance. - Highlights: • T-permeation has been measured on bare and coated type 321 SS containers. • Al{sub 2}O{sub 3}/FeAl coating give a reduction of T-permeability of 3 orders of magnitude. • Mechanism of Al{sub 2}O{sub 3}/FeAl barrier resisting T-permeation has obtained. • Quality of TPB is a very important factor for efficient T-permeating reduction.

  2. Permeation of Ionic Liquids through the skin

    Directory of Open Access Journals (Sweden)

    Ana Júlio

    2017-12-01

    Full Text Available Alternative forms of drug delivery such as delivery through the skin, have been developed to explore other routes. However, the incorporation of poorly soluble or partially insoluble drugs into these delivery systems represents a major problem. Ionic liquids (ILs may be incorporated in aqueous, oily or hydroalcoholic solutions and thus, may be used as excipients in drug delivery systems to increase/improve the topical and transdermal drug delivery. However, it is fundamental to consider the cytotoxicity of these salts and it is also crucial to evaluate if these compounds permeate through the skin. Herein, three imidazole-based ILs: [C2mim][Br], [C4mim][Br] and [C6mim][Br], were synthesized and each IL was incorporated within caffeine saturated solutions. Permeation studies of the active (caffeine in these solutions were performed to evaluate the amount of IL that permeated through the porcine ear skin in the presence of the active. To achieve this, gravimetric studies of the receptor compartment were performed. Results showed that the more lipophilic IL [C6mim][Br] presented the highest permeation through the skin. The permeation is dependent upon the size of the alkyl chain of the IL, and as more than 60% of the ILs permeate is it vital to consider the cytotoxicity of these salts when considering their incorporation in topical systems.

  3. Hydrogen permeation resistant layers for liquid metal reactors

    International Nuclear Information System (INIS)

    McGuire, J.C.

    1980-03-01

    Reviewing the literature in the tritium diffusion field one can readily see a wide divergence in results for both the response of permeation rate to pressure, and the effect of oxide layers on total permeation rates. The basic mechanism of protective oxide layers is discussed. Two coatings which are less hydrogen permeable than the best naturally occurring oxide are described. The work described is part of an HEDL-ANL cooperative research program on Tritium Permeation in Liquid Metal Cooled Reactors. This includes permeation work on hydrogen, deuterium, and tritium with the hydrogen-deuterium research leading to the developments presented

  4. Development of permeate flux model for municipal wastewater treatment using membrane bioreactor

    International Nuclear Information System (INIS)

    Geissler, S.; Zhou, H.; Zytner, R.; Melin, T.

    2002-01-01

    In municipal wastewater treatment, membrane filtration technologies receive great attention because they usually produce the better quality effluent, generate less sludge and require a smaller aeration tank volume. However, one main challenge of using membranes is membrane fouling, which results in a permeate flux decrease or transmembrane pressure increase over the time. Many efforts have been directed to develop the mechanistic permeate flux model to correlate the permeate flux with process parameters. However, their applicability has been largely thwarted due to complicated membrane fouling mechanisms and the interactions of many factors affecting the membrane bioreactor. This paper proposes a semi-empirical permeate flux model for the membrane bioreactor (MBR) process using ZENON immersed hollow fibre membrane modules. The semi-empirical model was proposed by assuming that the permeate flux is equal to transmembrane pressure divided by total resistance. The total resistance is divided into two components: an inside membrane resistance and an outer fouling layer resistance. These membrane resistances are then related to the ageing of membrane used. Good correlation was found between the predicted and measured flux, with the mean absolute deviation being less than 4%. The observations also identified some general rules for operating membrane systems. Ideally, it is advisable that high pressure periods be avoided as this leads to a faster increase of non-reversal membrane resistance. It was also observed that membrane preservatives should be washed out carefully prior to use. (author)

  5. Stability and oxygen permeation behavior of Ce0.8Sm0.2O2−δ–La0.8Sr0.2CrO3−δ composite membrane under large oxygen partial pressure gradients

    NARCIS (Netherlands)

    Wang, Bo; Yi, Jianxin; Winnubst, Aloysius J.A.; Chen, Chusheng

    2006-01-01

    The stability and oxygen permeation behavior of the Ce0.8Sm0.2O2−δ–La0.8Sr0.2CrO3−δ dual-phase composite were investigated under a large oxygen gradient with one side of it exposed to air and the other side to CO, CH4 or H2 at elevated temperatures. An oxygen permeation flux of 8.6 × 10−7 mol cm−2

  6. Tritium permeation losses in HYLIFE-II heat exchanger tubes

    International Nuclear Information System (INIS)

    Longhurst, G.R.; Dolan, T.J.

    1990-01-01

    Tritium permeation through the intermediate heat exchanger of the HYLIFE-II inertial fusion design concept is evaluated for routine operating conditions. The permeation process is modelled using the Lewis analogy combined with surface recombination. It is demonstrated that at very low driving potentials, permeation becomes proportional to the first power of the driving potential. The model predicts that under anticipated conditions the primary cooling loop will pass about 6% of the tritium entering it to the intermediate coolant. Possible approached to reducing tritium permeation are explored. Permeation is limited by turbulent diffusion transport through the molten salt. Hence, surface barriers with impendance factors typical of present technology can do very little to reduce permeation. Low Flibe viscosity is desirable. An efficient tritium removal system operating on the Flibe before it gets to the intermediate heat exchanger is required. Needs for further research are highlighted. 9 refs., 2 figs., 1 tab

  7. A permeation theory for single-file ion channels: one- and two-step models.

    Science.gov (United States)

    Nelson, Peter Hugo

    2011-04-28

    How many steps are required to model permeation through ion channels? This question is investigated by comparing one- and two-step models of permeation with experiment and MD simulation for the first time. In recent MD simulations, the observed permeation mechanism was identified as resembling a Hodgkin and Keynes knock-on mechanism with one voltage-dependent rate-determining step [Jensen et al., PNAS 107, 5833 (2010)]. These previously published simulation data are fitted to a one-step knock-on model that successfully explains the highly non-Ohmic current-voltage curve observed in the simulation. However, these predictions (and the simulations upon which they are based) are not representative of real channel behavior, which is typically Ohmic at low voltages. A two-step association/dissociation (A/D) model is then compared with experiment for the first time. This two-parameter model is shown to be remarkably consistent with previously published permeation experiments through the MaxiK potassium channel over a wide range of concentrations and positive voltages. The A/D model also provides a first-order explanation of permeation through the Shaker potassium channel, but it does not explain the asymmetry observed experimentally. To address this, a new asymmetric variant of the A/D model is developed using the present theoretical framework. It includes a third parameter that represents the value of the "permeation coordinate" (fractional electric potential energy) corresponding to the triply occupied state n of the channel. This asymmetric A/D model is fitted to published permeation data through the Shaker potassium channel at physiological concentrations, and it successfully predicts qualitative changes in the negative current-voltage data (including a transition to super-Ohmic behavior) based solely on a fit to positive-voltage data (that appear linear). The A/D model appears to be qualitatively consistent with a large group of published MD simulations, but no

  8. Effect of Permeation Enhancers on the Release Behavior and ...

    African Journals Online (AJOL)

    Purpose: The aim of this research work was to formulate, characterize and evaluate the in vitro permeation behavior of tramadol lotion containing propylene glycol (PG) and polyethylene glycol (PEG) as permeation enhancers. Methods: The permeation experiments were conducted in vitro using full thickness rabbit skin in ...

  9. Oxygen permeation flux through La1-ySryFeO3 limited by the carbon monoxide oxidation rate

    NARCIS (Netherlands)

    van Hassel, B.A.; van Hassel, B.A.; ten Elshof, Johan E.; Bouwmeester, Henricus J.M.

    1995-01-01

    The oxygen permeation flux through La1-ySryFeO3-δ (y = 0.1, 0.2) in a large oxygen partial pressure gradient (air/CO, CO2 mixture) was found to be limited by the carbon monoxide oxidation rate at the low oxygen partial pressure side of the membrane. The oxygen permeation flux through the membrane

  10. Modeling and experiments on tritium permeation in fusion reactor blankets

    Science.gov (United States)

    Holland, D. F.; Longhurst, G. R.

    The determination of tritium loss from helium-cooled fusion breeding blankets are discussed. The issues are: (1) applicability of present models to permeation at low tritium pressures; (2) effectiveness of oxide layers in reducing permeation; (3) effectiveness of hydrogen addition as a means to lower tritium permeation; and (4) effectiveness of conversion to tritiated water and subsequent trapping to reduce permeation. Theoretical models applicable to these issues are discussed, and results of experiments in two areas are presented; permeation of mixtures of hydrogen isotopes and conversion to tritiated water.

  11. Modeling and experiments on tritium permeation in fusion reactor blankets

    International Nuclear Information System (INIS)

    Holland, D.F.; Longhurst, G.R.

    1985-01-01

    Issues are discussed that are critical in determining tritium loss from helium-cooled fusion breeding blankets. These issues are: (a) applicability of present models to permeation at low tritium pressures, (b) effectiveness of oxide layers in reducing permeation, (c) effectiveness of hydrogen addition as a means to lower tritium permeation, and (d) effectiveness of conversion to tritiated water and subsequent trapping as a means to reduce permeation. The paper discusses theoretical models applicable to these issues, and presents results of experiments in two areas: permeation of mixtures of hydrogen isotopes and conversion to tritiated water

  12. Measurement of skin permeation/penetration of nanoparticles for their safety evaluation.

    Science.gov (United States)

    Kimura, Eriko; Kawano, Yuichiro; Todo, Hiroaki; Ikarashi, Yoshiaki; Sugibayashi, Kenji

    2012-01-01

    The aim of the present study was to quantitatively evaluate the skin permeation/penetration of nanomaterials and to consider their penetration pathway through skin. Firstly, penetration/permeation of a model fluorescent nanoparticle, Fluoresbrite®, was determined through intact rat skin and several damaged skins. Fluoresbrite® permeated through only needle-punctured skin. The permeation profiles of soluble high molecular compounds, fluorescein isothiocyanate-dextrans (FITC-dextrans, FDs), with different molecular weights were also measured for comparison. The effects of molecular sizes and different skin pretreatments on the skin barrier were determined on the skin penetration/permeation of Fluoresbrite® and FDs. Fluoresbrite® was not permeated the intact skin, but FDs were permeated the skin. The skin distribution of titanium dioxide and zinc oxide nanoparticles was also observed after topical application of commercial cosmetics. Nanoparticles in sunscreen cosmetics were easily distributed into the groove and hair follicles after their topical application, but seldom migrated from the groove or follicles to viable epidermis and dermis. The obtained results suggested that nanoparticles did not permeate intact skin, but permeated pore-created skin. No or little permeation was observed for these nanomaterials through the stratum corneum.

  13. Gas-driven permeation of deuterium through tungsten and tungsten alloys

    Energy Technology Data Exchange (ETDEWEB)

    Buchenauer, Dean A., E-mail: dabuche@sandia.gov [Sandia National Laboratories, Energy Innovation Department, Livermore, CA 94550 (United States); Karnesky, Richard A. [Sandia National Laboratories, Energy Innovation Department, Livermore, CA 94550 (United States); Fang, Zhigang Zak; Ren, Chai [University of Utah, Department of Metallurgical Engineering, Salt Lake City, UT 84112 (United States); Oya, Yasuhisa [Shizuoka University, Graduate School of Science, Shizuoka (Japan); Otsuka, Teppei [Kyushu University, Department of Advanced Energy Engineering Science, Fukuoka (Japan); Yamauchi, Yuji [Hokkaido University, Third Division of Quantum Science and Engineering, Faculty of Engineering, Sapporo (Japan); Whaley, Josh A. [Sandia National Laboratories, Energy Innovation Department, Livermore, CA 94550 (United States)

    2016-11-01

    Highlights: • We have designed and performed initial studies on a high temperature gas-driven permeation cell capable of operating at temperatures up to 1150 °C and at pressures between 0.1–1 atm. • Permeation measurements on ITER grade tungsten compare well with past studies by Frauenfelder and Zahkarov in the temperature range from 500 to 1000 °C. • First permeation measurements on Ti dispersoid-strengthened ultra-fine grained tungsten show higher permeation at 500 °C, but very similar permeation with ITER tungsten at 1000 °C. Diffusion along grain boundaries may be playing a role for this type of material. - Abstract: To address the transport and trapping of hydrogen isotopes, several permeation experiments are being pursued at both Sandia National Laboratories (deuterium gas-driven permeation) and Idaho National Laboratories (tritium gas- and plasma-driven tritium permeation). These experiments are in part a collaboration between the US and Japan to study the performance of tungsten at divertor relevant temperatures (PHENIX). Here we report on the development of a high temperature (≤1150 °C) gas-driven permeation cell and initial measurements of deuterium permeation in several types of tungsten: high purity tungsten foil, ITER-grade tungsten (grains oriented through the membrane), and dispersoid-strengthened ultra-fine grain (UFG) tungsten being developed in the US. Experiments were performed at 500–1000 °C and 0.1–1.0 atm D{sub 2} pressure. Permeation through ITER-grade tungsten was similar to earlier W experiments by Frauenfelder (1968–69) and Zaharakov (1973). Data from the UFG alloy indicates marginally higher permeability (< 10×) at lower temperatures, but the permeability converges to that of the ITER tungsten at 1000 °C. The permeation cell uses only ceramic and graphite materials in the hot zone to reduce the possibility for oxidation of the sample membrane. Sealing pressure is applied externally, thereby allowing for elevation

  14. Permeation of aromatic solvent mixtures through nitrile protective gloves.

    Science.gov (United States)

    Chao, Keh-Ping; Hsu, Ya-Ping; Chen, Su-Yi

    2008-05-30

    The permeation of binary and ternary mixtures of benzene, toluene, ethyl benzene and p-xylene through nitrile gloves were investigated using the ASTM F739 test cell. The more slowly permeating component of a mixture was accelerated to have a shorter breakthrough time than its pure form. The larger differences in solubility parameter between a solvent mixture and glove resulted in a lower permeation rate. Solubility parameter theory provides a potential approach to interpret the changes of permeation properties for BTEX mixtures through nitrile gloves. Using a one-dimensional diffusion model based on Fick's law, the permeation concentrations of ASTM F739 experiments were appropriately simulated by the estimated diffusion coefficient and solubility. This study will be a fundamental work for the risk assessment of the potential dermal exposure of workers wearing protective gloves.

  15. Permeation of deuterium implanted into vanadium alloys

    International Nuclear Information System (INIS)

    Anderl, R.A.; Longhurst, G.R.; Struttmann, D.A.

    1986-05-01

    Permeation of deuterium through the vanadium alloy, V-15Cr-5Ti, was investigated using 3-keV, D 3 + ion beams from a small accelerator. The experiments consisted of measurements of the deuterium reemission and permeation rates as a function of implantation fluence for 0.5-mm thick specimens heated to tempertures from 623 to 823 0 K. Implantation-side surface characterization was made by simultaneous measurements of sputtered ions with a secondary ion mass spectrometer (SIMS). Analyses of these measurements indicate that for the experimental conditions used, the steady-state deuterium permeation flux in V-15Cr-5Ti is approximately 18% of the implantation flux. This corresponds to approximately 1000 times that seen in the ferritic steel, HT-9, under comparable conditions. Measurement of deuterium diffusivity in V-15Cr-5Ti using permeation break-through times indicates D = 1.4 x 10 -8 exp(-.11 eV/kT) (m 2 /s)

  16. Hydrogen permeation properties of plasma-sprayed tungsten*1

    Science.gov (United States)

    Anderl, R. A.; Pawelko, R. J.; Hankins, M. R.; Longhurst, G. R.; Neiser, R. A.

    1994-09-01

    Tungsten has been proposed as a plasma-facing component material for advanced fusion facilities. This paper reports on laboratory-scale studies that were done to assess the hydrogen permeation properties of plasma-sprayed tungsten for such applications. The work entailed deuterium permeation measurements for plasma-sprayed (PS) tungsten coatings, sputter-deposited (SP) tungsten coatings, and steel substrate material using a mass-analyzed, 3 keV D 3+ ion beam with fluxes of ˜6.5 × 10 19 D/m 2 s. Extensive characterization analyses for the plasma-sprayed tungsten coatings were made using Auger spectrometry and scanning electron microscopy (SEM). Observed permeation rates through composite PS-tungsten/steel specimens were several orders of magnitude below the permeation levels observed for SP-tungsten/steel composite specimens and pure steel specimens. Characterization analyses indicated that the plasma-sprayed tungsten coating had a nonhomogeneous microstructure that consisted of splats with columnar solidification, partially-melted particles with grain boundaries, and void regions. Reduced permeation levels can be attributed to the complex microstructure and a substantial surface-connected porosity.

  17. Surface oxygen vacancy and oxygen permeation flux limits of perovskite ion transport membranes

    KAUST Repository

    Hunt, Anton

    2015-09-01

    © 2015 Elsevier B.V. The mechanisms and quantitative models for how oxygen is separated from air using ion transport membranes (ITMs) are not well understood, largely due to the experimental complexity for determining surface exchange reactions at extreme temperatures (>800°C). This is especially true when fuels are present at the permeate surface. For both inert and reactive (fuels) operations, solid-state oxygen surface vacancies (δ) are ultimately responsible for driving the oxygen flux, JO2. In the inert case, the value of δ at either surface is a function of the local PO2 and temperature, whilst the magnitude of δ dictates both the JO2 and the inherent stability of the material. In this study values of δ are presented based on experimental measurements under inert (CO2) sweep: using a permeation flux model and local PO2 measurements, collected by means of a local gas-sampling probe in our large-scale reactor, we can determine δ directly. The ITM assessed was La0.9Ca0.1FeO3-δ (LCF); the relative resistances to JO2 were quantified using the pre-defined permeation flux model and local PO2 values. Across a temperature range from 825°C to 1056°C, δ was found to vary from 0.007 to 0.029 (<1%), safely within material stability limits, whilst the permeate surface exchange resistance dominates. An inert JO2 limit was identified owing to a maximum sweep surface δ, δmaxinert. The physical presence of δmaxinert is attributed to a rate limiting step shift from desorption to associative electron transfer steps on the sweep surface as PO2 is reduced. Permeate surface exchange limitations under non-reactive conditions suggest that reactive (fuel) operation is necessary to accelerate surface chemistry for future work, to reduce flux resistance and push δpast δmaxinert in a stable manner.

  18. Hydrogen permeation preventive structural materials

    International Nuclear Information System (INIS)

    Fukushima, Kimichika; Nakahigashi, Shigeo; Imura, Masashi; Terasawa, Michitaka; Ebisawa, Katsuyuki.

    1986-01-01

    Purpose: To provide highly practical wall materials for use in thermonuclear reactors capable of effectively preventing the permeation of hydrogen isotopes such as tritium thereby preventing the contamination of coolants. Constitution: Helium gas is injected into or at the surface of base materials comprising stainless steel plates to form a helium gas region. Alternatively, boron, nitrogen or the compound thereof having a greater helium forming nuclear reaction cross section than that of the base materials is mixed or injected into the base material to form the helium gas region through (n,α) reaction under neutron irradiation. Since the helium gas region constitutes a diffusion barrier for the tritium as the hydrogen isotope, the permeation amount of tritium is significantly suppressed. Helium gas bubbles or lattice defects are formed in the helium gas region under the neutron irradiation, by which the hydrogen isotope capturing effect can also be effected. In this way, permeation of the hydrogen isotope, contamination of the coolants, etc. can be prevented to provide great practical effectives. (Kawakami, Y.)

  19. Reemission and permeation of deuterium implanted into metals

    International Nuclear Information System (INIS)

    Tanabe, T.; Furuyama, Y.; Imoto, S.

    1984-01-01

    Focusing on the marked depression of deuterium permeation rate during the deuteron bombardment, implantation experiments coupled with gaseous permeation experiments are performed on pure Ni and Ni with evaporated MnO. It is concluded that the reemission of implanted deuterium is initially depressed, but it soon becomes enhanced with increase of fluence leading to a rapid decrease of permeation rate at the intermediate temperatures 600-1000 K, which is attributed to the formation of short diffusion paths from the projected range to the front surface. (orig.)

  20. Synthesis of conjugated chitosan and its effect on drug permeation from transdermal patches.

    Science.gov (United States)

    Satheeshababu, B K; Shivakumar, K L

    2013-03-01

    The aim of this study was to synthesis the conjugated chitosan by covalent attachment of thiol moieties to the cationic polymer, mediated by a carbodiimide to improve permeation properties of chitosan. Thioglycolic acid was covalently attached to chitosan by the formation of amide bonds between the primary amino groups of the polymer and the carboxylic acid groups of thioglycolic acid. Hence, these polymers are called as thiomers or thiolated polymers. Conjugation of chitosan was confirmed by Fourier transform-infrared and differential scanning calorimetric analysis. Matrix type transdermal patches of carvedilol were prepared using the different proportions of chitosan and chitosan-thioglycolic acid conjugates (2:0, 1.7:0.3, 1.4:0.6, 1:1, 0.6:1.4 and 0.3:1.7) by solvent casting technique. Prepared matrix type patches were evaluated for their physicochemical characterization followed by in vitro evaluation. Selected formulations were subjected for their ex vivo studies on Wistar albino rat skin and human cadaver skin using the modified Franz diffusion cell. As the proportion of conjugated chitosan increased, the transdermal patches showed increased drug permeation. The mechanism of drug release was found to be nonFickian profiles. The present study concludes that the transdermal patches of carvedilol using conjugated chitosan with different proportions of chitosan were successfully developed to provide improved drug permeation. The transdermal patches can be a good approach to improve drug bioavailability by bypassing the extensive hepatic first-pass metabolism of the drug.

  1. Development of a Contact Permeation Test Fixture and Method

    Science.gov (United States)

    2013-04-01

    Permeation and Analytical Solutions Team Quality System documentation and the guidance found in the ISO 17025 standard. All permeation and...annular ring (left) and no pressure (right). 2.2.4 Quality Controls Analytical permeation testing was conducted in accordance with ISO 17025 quality...internal standard. This mixture was vortexed for 20–30 s then centrifuged at 15,000 rpm for 5 min in a Micromax microcentrifuge (Thermo IEC ; Needham

  2. Measurement of Skin Permeation/Penetration of Nanoparticles for Their Safety Evaluation

    OpenAIRE

    木村, 恵理子; 河野, 雄一郎; 藤堂, 浩明; 五十嵐, 良明; 杉林, 堅次

    2012-01-01

    The aim of the present study was to quantitatively evaluate the skin permeation/penetration of nanomaterials and to consider their penetration pathway through skin. Firstly, penetration/permeation of a model fluorescent nanoparticle, Fluoresbrite?, was determined through intact rat skin and several damaged skins. Fluoresbrite? permeated through only needle-punctured skin. The permeation profiles of soluble high molecular compounds, fluorescein isothiocyanate-dextrans (FITC-dextrans, FDs), wit...

  3. Permeation enhancer strategies in transdermal drug delivery.

    Science.gov (United States)

    Marwah, Harneet; Garg, Tarun; Goyal, Amit K; Rath, Goutam

    2016-01-01

    Today, ∼74% of drugs are taken orally and are not found to be as effective as desired. To improve such characteristics, transdermal drug delivery was brought to existence. This delivery system is capable of transporting the drug or macromolecules painlessly through skin into the blood circulation at fixed rate. Topical administration of therapeutic agents offers many advantages over conventional oral and invasive techniques of drug delivery. Several important advantages of transdermal drug delivery are prevention from hepatic first pass metabolism, enhancement of therapeutic efficiency and maintenance of steady plasma level of the drug. Human skin surface, as a site of drug application for both local and systemic effects, is the most eligible candidate available. New controlled transdermal drug delivery systems (TDDS) technologies (electrically-based, structure-based and velocity-based) have been developed and commercialized for the transdermal delivery of troublesome drugs. This review article covers most of the new active transport technologies involved in enhancing the transdermal permeation via effective drug delivery system.

  4. Effect of residual stresses on hydrogen permeation in iron

    International Nuclear Information System (INIS)

    Mouanga, M.; Bercot, P.; Takadoum, J.

    2010-01-01

    The effect of residual stresses on electrochemical permeation in iron membrane was investigated. Four thermal and mechanical treatments were chosen to obtain different surface states in relation to the residual stresses. Residual stresses were determined by X-ray diffraction (XRD) using the Macherauch and Mueller method. The results were completed by the microhardness measurements. For all iron membranes, compressive residual stresses were obtained. Electrochemical permeation experiments using a Devanathan and Stachurski cell were employed to determine the hydrogen permeation behaviour of the various iron membranes. The latter was charged with hydrogen by galvanostatic cathodic polarization in 0.1 M NaOH at 25 deg. C. The experimental results revealed that hydrogen permeation rate increases with increasing residual stresses introduced in iron membranes.

  5. Design and tritium permeation analysis of China HCCB TBM port cell

    International Nuclear Information System (INIS)

    Jiangfeng, S.; Guoqiang, H.; Zhiyong, H.; Chang'an, C.; Deli, L.

    2015-01-01

    China is planning to develop a helium-cooled ceramic breeder (HCCB) test blanket module (TBM) on ITER to test key blanket technologies. In this paper, the design and tritium permeation analysis of China HCCB TBM port cell are introduced. A theoretical model has been developed to estimate tritium permeation rates and leak rates from the components and pipes which China has scheduled to house in the port cell. It is shown that on normal working conditions, the permeation and leak rate of the systems in the port cell will be no higher than 1.58 Ci/d without the use of tritium permeation barriers, and 0.10 Ci/d with the use of tritium permeation barriers. It also appears that tritium permeation barriers are necessary for high temperature components such as the reduction bed and the heater

  6. Design and tritium permeation analysis of China HCCB TBM port cell

    Energy Technology Data Exchange (ETDEWEB)

    Jiangfeng, S.; Guoqiang, H.; Zhiyong, H.; Chang' an, C.; Deli, L. [China Academy of Engineering Physics, Mianyang, Sichuan (China)

    2015-03-15

    China is planning to develop a helium-cooled ceramic breeder (HCCB) test blanket module (TBM) on ITER to test key blanket technologies. In this paper, the design and tritium permeation analysis of China HCCB TBM port cell are introduced. A theoretical model has been developed to estimate tritium permeation rates and leak rates from the components and pipes which China has scheduled to house in the port cell. It is shown that on normal working conditions, the permeation and leak rate of the systems in the port cell will be no higher than 1.58 Ci/d without the use of tritium permeation barriers, and 0.10 Ci/d with the use of tritium permeation barriers. It also appears that tritium permeation barriers are necessary for high temperature components such as the reduction bed and the heater.

  7. Permeation barrier properties of thin oxide films on flexible polymer substrates

    International Nuclear Information System (INIS)

    Fahlteich, John; Fahland, Matthias; Schoenberger, Waldemar; Schiller, Nicolas

    2009-01-01

    Solar cells and organic electronic devices require an encapsulation to ensure sufficient lifetime. Key parameters of the encapsulation are permeation barrier, UV stability, temperature stability, optical transmission spectra and mechanical stability. The requirements depend very much on the specific application. Many work groups suggest multilayer stacks to meet the permeation requirements. In this paper the permeation barrier properties of the different constituents of such a multilayer stack are characterized. Different layer materials are compared regarding their water vapour and oxygen permeability as well as the influence of process parameters is examined. Finally temperature dependent permeation measurements are used to characterize the permeation mechanisms in the different constituents of the multilayer barrier

  8. Separation of aromatics by vapor permeation through solvent swollen membrane

    Energy Technology Data Exchange (ETDEWEB)

    Ito, A.; Adachi, K.; Feng, Y. [Niigata University, Niigata (Japan)

    1995-12-20

    A vapor permeation process for aromatics separation from a hydrocarbon mixture was studied by means of the simultaneous permeation of dimethylsulfoxide vapor as an agent for membrane swelling and preferential permeation of aromatics. The separation performance of the process was demonstrated by a polyvinylalcohol membrane for mixed vapors of benzene/cyclohexane, xylene/octane and a model gasoline. The aromatic vapors preferentially permeated from these mixed vapor feeds. The separation factor was over 10. The separation mechanism of the process mainly depends on the relative salability of the vapors between aromatics and other hydrocarbons in dimethylsulfoxide. 14 refs., 9 figs., 1 tab.

  9. Permeation barrier for lightweight liquid hydrogen tanks

    Energy Technology Data Exchange (ETDEWEB)

    Schultheiss, D.

    2007-04-16

    For the future usage of hydrogen as an automotive fuel, its on-board storage is crucial. One approach is the storage of liquid hydrogen (LH2, 20 K) in double-walled, vacuum insulated tanks. The introduction of carbon fiber reinforced plastics (CFRP) as structural material enables a high potential of reducing the weight in comparison to the state-of-the-art stainless steel tanks. The generally high permeability of hydrogen through plastics, however, can lead to long-term degradation of the insulating vacuum. The derived objective of this dissertation was to find and apply an adequate permeation barrier (liner) on CFRP. The investigated liners were either foils adhered on CFRP specimens or coatings deposited on CFRP specimens. The coatings were produced by means of thermal spraying, metal plating or physical vapor deposition (PVD). The materials of the liners included Al, Au, Cu, Ni and Sn as well as stainless steel and diamond-like carbon. The produced liners were tested for their permeation behavior, thermal shock resistance and adherence to the CFRP substrate. Additionally, SEM micrographs were used to characterize and qualify the liners. The foils, although being a good permeation barrier, adhered weakly to the substrate. Furthermore, leak-free joining of foil segments is a challenge still to be solved. The metal plating liners exhibited the best properties. For instance, no permeation could be detected through a 50 {mu}m thick Cu coating within the accuracy of the measuring apparatus. This corresponds to a reduction of the permeation gas flow by more than factor 7400 compared to uncoated CFRP. In addition, the metal platings revealed a high adherence and thermal shock resistance. The coatings produced by means of thermal spraying and PVD did not show a sufficient permeation barrier effect. After having investigated the specimens, a 170 liter CFRP tank was fully coated with 50 {mu}m Cu by means of metal plating. (orig.)

  10. Simplified tritium permeation model

    International Nuclear Information System (INIS)

    Longhurst, G.R.

    1993-01-01

    In this model I seek to provide a simplified approach to solving permeation problems addressed by TMAP4. I will assume that there are m one-dimensional segments with thickness L i , i = 1, 2, hor-ellipsis, m, joined in series with an implantation flux, J i , implanting at the single depth, δ, in the first segment. From material properties and heat transfer considerations, I calculate temperatures at each face of each segment, and from those temperatures I find local diffusivities and solubilities. I assume recombination coefficients K r1 and K r2 are known at the upstream and downstream faces, respectively, but the model will generate Baskes recombination coefficient values on demand. Here I first develop the steady-state concentration equations and then show how trapping considerations can lead to good estimates of permeation transient times

  11. Permeation of limonene through disposable nitrile gloves using a dextrous robot hand.

    Science.gov (United States)

    Banaee, Sean; S Que Hee, Shane

    2017-03-28

    The purpose of this study was to investigate the permeation of the low-volatile solvent limonene through different disposable, unlined, unsupported, nitrile exam whole gloves (blue, purple, sterling, and lavender, from Kimberly-Clark). This study utilized a moving and static dextrous robot hand as part of a novel dynamic permeation system that allowed sampling at specific times. Quantitation of limonene in samples was based on capillary gas chromatography-mass spectrometry and the internal standard method (4-bromophenol). The average post-permeation thicknesses (before reconditioning) for all gloves for both the moving and static hand were more than 10% of the pre-permeation ones (P≤0.05), although this was not so on reconditioning. The standardized breakthrough times and steady-state permeation periods were similar for the blue, purple, and sterling gloves. Both methods had similar sensitivity. The lavender glove showed a higher permeation rate (0.490±0.031 μg/cm 2 /min) for the moving robotic hand compared to the non-moving hand (P≤0.05), this being ascribed to a thickness threshold. Permeation parameters for the static and dynamic robot hand models indicate that both methods have similar sensitivity in detecting the analyte during permeation and the blue, purple, and sterling gloves behave similarly during the permeation process whether moving or non-moving.

  12. Permeation of limonene through disposable nitrile gloves using a dextrous robot hand

    Science.gov (United States)

    Banaee, Sean; S Que Hee, Shane

    2017-01-01

    Objectives: The purpose of this study was to investigate the permeation of the low-volatile solvent limonene through different disposable, unlined, unsupported, nitrile exam whole gloves (blue, purple, sterling, and lavender, from Kimberly-Clark). Methods: This study utilized a moving and static dextrous robot hand as part of a novel dynamic permeation system that allowed sampling at specific times. Quantitation of limonene in samples was based on capillary gas chromatography-mass spectrometry and the internal standard method (4-bromophenol). Results: The average post-permeation thicknesses (before reconditioning) for all gloves for both the moving and static hand were more than 10% of the pre-permeation ones (P≤0.05), although this was not so on reconditioning. The standardized breakthrough times and steady-state permeation periods were similar for the blue, purple, and sterling gloves. Both methods had similar sensitivity. The lavender glove showed a higher permeation rate (0.490±0.031 μg/cm2/min) for the moving robotic hand compared to the non-moving hand (P≤0.05), this being ascribed to a thickness threshold. Conclusions: Permeation parameters for the static and dynamic robot hand models indicate that both methods have similar sensitivity in detecting the analyte during permeation and the blue, purple, and sterling gloves behave similarly during the permeation process whether moving or non-moving. PMID:28111415

  13. Deuterium permeation and diffusion in high-purity beryllium

    International Nuclear Information System (INIS)

    Abramov, E.; Riehm, M.P.; Thompson, D.A.; Smeltzer, W.W.

    1990-01-01

    The permeation rate of deuterium through high-purity beryllium membranes was measured using the gas-driven permeation technique. The time-dependent and the steady-state deuterium flux data were analyzed and the effective diffusivities of the samples were determined. Using multilayer permeation theory the effects of surface oxide were eliminated and the diffusion coefficients of the bulk beryllium determined. The diffusion parameters obtained for the extra-grade beryllium samples (99.8%) are D 0 =6.7x10 -9 m 2 /s and E D =28.4 kJ/mol. For the high-grade beryllium samples (99%) the parameters are D 0 =8.0x10 -9 m 2 /s and E D =35.1 kJ/mol. (orig.)

  14. Recent results on implantation and permeation into fusion reactor materials

    Science.gov (United States)

    Anderl, R. A.; Holland, D. F.; Longhurst, G. R.; Struttman, D. A.

    This paper reports on implantation-driven permeation experiments that have been made for primary candidate alloy (PCA) and the ferritic steel HT-9 using deuterium ion beams from an accelerator. The results include measurements of the implantation flux and fluence dependence of the deuterium reemission and permeation for specimens heated to approximately 430(0)C. Simultaneous measurements of the ions sputtered from the specimen front surface with a secondary ion mass spectrometer provided some characterization of the surface condition throughout an experiment. For both materials, the permeation rate was lowered by the implantation process. However, the steady state permeation rate for HT-9 was found to be at least a factor of 5 greater than that for PCA.

  15. Towards a unified model of passive drug permeation I: origins of the unstirred water layer with applications to ionic permeation.

    Science.gov (United States)

    Ghosh, Avijit; Scott, Dennis O; Maurer, Tristan S

    2014-02-14

    In this work, we provide a unified theoretical framework describing how drug molecules can permeate across membranes in neutral and ionized forms for unstirred in vitro systems. The analysis provides a self-consistent basis for the origin of the unstirred water layer (UWL) within the Nernst-Planck framework in the fully unstirred limit and further provides an accounting mechanism based simply on the bulk aqueous solvent diffusion constant of the drug molecule. Our framework makes no new assumptions about the underlying physics of molecular permeation. We hold simply that Nernst-Planck is a reasonable approximation at low concentrations and all physical systems must conserve mass. The applicability of the derived framework has been examined both with respect to the effect of stirring and externally applied voltages to measured permeability. The analysis contains data for 9 compounds extracted from the literature representing a range of permeabilities and aqueous diffusion coefficients. Applicability with respect to ionized permeation is examined using literature data for the permanently charged cation, crystal violet, providing a basis for the underlying mechanism for ionized drug permeation for this molecule as being due to mobile counter-current flow. Copyright © 2013 Elsevier B.V. All rights reserved.

  16. Electrochemical measurement of tritium and hydrogen permeation through iron membranes

    International Nuclear Information System (INIS)

    Hagi, Hideki; Hayashi, Yasunori

    1987-01-01

    Permeation rates of tritium and hydrogen through iron were measured by the electro-chemical method in which an aqueous solution containing 3.7 x 10 12 Bq/m 3 tritium was used as a cathodic electrolyte. Tritium and hydrogen were introduced from one side of a specimen by cathodic polarization with a constant current density, while at the other side of the specimen the permeated tritium and hydrogen were extracted by potentiostatical ionization. Nearly all of the potentiostatic current on the extraction side is produced by the ionization of hydrogen, because the concentration of tritium in the cathodic electrolyte is very small. The amount of permeated hydrogen was obtained by integrating the potentiostatic current, and that of permeated tritium was determined by measuring the radioactivity of the electrolyte sampled from the anodic side. The separation factor for permeation obtained under steady state conditions (the ratio of permeation rates of hydrogen to tritium divided by the ratio of the concentration of hydrogen to tritium in the cathodic electrolyte) is 12 at 288 K. This value is independent of cathodic current density. Diffusion coefficients of tritium (D T ) and hydrogen (D H ) in iron were determined from the tritium and hydrogen permeation by using time lag technique. For annealed iron at 286 K, D T = 9 x 10 -10 m 2 /s and D H = 4 x 10 -9 m 2 /s, and for 9 % cold-worked iron at 284 K, D T = 3 x 10 -10 m 2 /s and D H = 4 x 10 -10 m 2 /s. (author)

  17. Compensation effect in H 2 permeation kinetics of PdAg membranes

    KAUST Repository

    Zeng, Gaofeng

    2012-08-30

    Knowledge about the (inter)dependence of permeation kinetic parameters on the stoichiometry of H 2-selective alloys is still rudimentary, although uncovering the underlying systematic correlations will greatly facilitate current efforts into the design of novel high-performance H 2 separation membranes. Permeation measurements with carefully engineered, 2-7 μm thick supported Pd 100-xAg x membranes reveal that the activation energy and pre-exponential factor of H 2 permeation laws vary systematically with alloy composition, and both kinetic parameters are strongly correlated for x ≤ 50. We show that this permeation kinetic compensation effect corresponds well with similar correlations in the hydrogen solution thermodynamics and diffusion kinetics of PdAg alloys that govern H 2 permeation rates. This effect enables the consistent description of permeation characteristics over wide temperature and alloy stoichiometry ranges, whereas hydrogen solution thermodynamics may play a role, too, as a yet unrecognized source of kinetic compensation in, for example, H 2-involving reactions over metal catalysts or hydrogenation/ dehydrogenation of hydrogen storage materials. © 2012 American Chemical Society.

  18. Hydrogen gas driven permeation through tungsten deposition layer formed by hydrogen plasma sputtering

    International Nuclear Information System (INIS)

    Uehara, Keiichiro; Katayama, Kazunari; Date, Hiroyuki; Fukada, Satoshi

    2015-01-01

    Highlights: • H permeation tests for W layer formed by H plasma sputtering are performed. • H permeation flux through W layer is larger than that through W bulk. • H diffusivity in W layer is smaller than that in W bulk. • The equilibrium H concentration in W layer is larger than that in W bulk. - Abstract: It is important to evaluate the influence of deposition layers formed on plasma facing wall on tritium permeation and tritium retention in the vessel of a fusion reactor from a viewpoint of safety. In this work, tungsten deposition layers having different thickness and porosity were formed on circular nickel plates by hydrogen RF plasma sputtering. Hydrogen permeation experiment was carried out at the temperature range from 250 °C to 500 °C and at hydrogen pressure range from 1013 Pa to 101,300 Pa. The hydrogen permeation flux through the nickel plate with tungsten deposition layer was significantly smaller than that through a bare nickel plate. This indicates that a rate-controlling step in hydrogen permeation was not permeation through the nickel plate but permeation though the deposition layer. The pressure dependence on the permeation flux differed by temperature. Hydrogen permeation flux through tungsten deposition layer is larger than that through tungsten bulk. From analysis of the permeation curves, it was indicated that hydrogen diffusivity in tungsten deposition layer is smaller than that in tungsten bulk and the equilibrium hydrogen concentration in tungsten deposition layer is enormously larger than that in tungsten bulk at same hydrogen pressure.

  19. Large-scale transcriptome analysis reveals arabidopsis metabolic pathways are frequently influenced by different pathogens.

    Science.gov (United States)

    Jiang, Zhenhong; He, Fei; Zhang, Ziding

    2017-07-01

    Through large-scale transcriptional data analyses, we highlighted the importance of plant metabolism in plant immunity and identified 26 metabolic pathways that were frequently influenced by the infection of 14 different pathogens. Reprogramming of plant metabolism is a common phenomenon in plant defense responses. Currently, a large number of transcriptional profiles of infected tissues in Arabidopsis (Arabidopsis thaliana) have been deposited in public databases, which provides a great opportunity to understand the expression patterns of metabolic pathways during plant defense responses at the systems level. Here, we performed a large-scale transcriptome analysis based on 135 previously published expression samples, including 14 different pathogens, to explore the expression pattern of Arabidopsis metabolic pathways. Overall, metabolic genes are significantly changed in expression during plant defense responses. Upregulated metabolic genes are enriched on defense responses, and downregulated genes are enriched on photosynthesis, fatty acid and lipid metabolic processes. Gene set enrichment analysis (GSEA) identifies 26 frequently differentially expressed metabolic pathways (FreDE_Paths) that are differentially expressed in more than 60% of infected samples. These pathways are involved in the generation of energy, fatty acid and lipid metabolism as well as secondary metabolite biosynthesis. Clustering analysis based on the expression levels of these 26 metabolic pathways clearly distinguishes infected and control samples, further suggesting the importance of these metabolic pathways in plant defense responses. By comparing with FreDE_Paths from abiotic stresses, we find that the expression patterns of 26 FreDE_Paths from biotic stresses are more consistent across different infected samples. By investigating the expression correlation between transcriptional factors (TFs) and FreDE_Paths, we identify several notable relationships. Collectively, the current study

  20. Deuterium permeation and diffusion in high purity beryllium

    International Nuclear Information System (INIS)

    Abramov, E.

    1990-05-01

    The permeation rate of deuterium through high-purity beryllium membranes was measured using the gas-driven permeation technique. The time-dependent and the steady-state deuterium flux data were analyzed and the effective diffusivities of the samples were determined. A multilayer permeation theory was used in order to eliminate the surface oxide effects and the diffusion coefficients of the bulk beryllium were determined. The diffusion parameters obtained for the extra-grade beryllium samples (99.8%) are D 0 = 6.7 x 10 -9 [m 2 /s] and E D = 28.4 [KJ/mol]; and for the high-grade beryllium samples (99%) the parameters are D 0 = 8.0 x 10 -9 [m 2 /s] and E D = 35.1 [KJ/mol

  1. Hydrogen ion-driven permeation in carbonaceous films

    International Nuclear Information System (INIS)

    Anderl, R.A.; Holland, D.F.; Longhurst, G.R.

    1989-01-01

    This paper presents the results of investigations into the permeation properties of amorphous carbonaceous, a-C:H, films produced by plasmachemical deposition techniques. Carbonaceous films on iron substrates with thickness ranging from 60 nm to 110 nm were subjected to high fluence implantations with mass analyzed D 3 + ions with energies ranging from 600 eV to 3000 eV and fluxes ranging from 5x10 14 D/cm 2 s to 5x10 15 D/cm 2 s, respectively. Deuterium re-emission upstream, deuterium permeation downstream and secondary ions sputtered from the implantation surface were measured as a function of implantation fluence for specimens at 420 K. The present studies indicate that the a-C:H film permeability is directly related to the time, hence the fluence, required to achieve isotopic replacement and saturation of the deuterium ion beam atoms stopped in the implant region. Once the deuterium saturation level is achieved in the layer, a significant fraction of the implanting ions can result in permeation. For the present experiment, this permeation factor was much higher than that for uncoated iron specimens subjected to similar beam conditions. Carbon sputter yields of 0.008-0.01 C/D were determined in this work for 1000-eV to 400-eV deuterium ions incident on a-C:H films. (orig.)

  2. Hydrogen ion-driven permeation in carbonaceous films

    Energy Technology Data Exchange (ETDEWEB)

    Anderl, R.A.; Holland, D.F.; Longhurst, G.R.

    1989-04-01

    This paper presents the results of investigations into the permeation properties of amorphous carbonaceous, a-C:H, films produced by plasmachemical deposition techniques. Carbonaceous films on iron substrates with thickness ranging from 60 nm to 110 nm were subjected to high fluence implantations with mass analyzed D/sub 3//sup +/ ions with energies ranging from 600 eV to 3000 eV and fluxes ranging from 5x10/sup 14/ D/cm/sup 2/ s to 5x10/sup 15/ D/cm/sup 2/ s, respectively. Deuterium re-emission upstream, deuterium permeation downstream and secondary ions sputtered from the implantation surface were measured as a function of implantation fluence for specimens at 420 K. The present studies indicate that the a-C:H film permeability is directly related to the time, hence the fluence, required to achieve isotopic replacement and saturation of the deuterium ion beam atoms stopped in the implant region. Once the deuterium saturation level is achieved in the layer, a significant fraction of the implanting ions can result in permeation. For the present experiment, this permeation factor was much higher than that for uncoated iron specimens subjected to similar beam conditions. Carbon sputter yields of 0.008-0.01 C/D were determined in this work for 1000-eV to 400-eV deuterium ions incident on a-C:H films. (orig.).

  3. Hydrogen ion-driven permeation in carbonaceous films

    Science.gov (United States)

    Anderl, R. A.; Holland, D. F.; Longhurst, G. R.

    1989-04-01

    This paper presents the results of investigations into the permeation properties of amorphous carbonaceous, a-C: H, films produced by plasmachemical deposition techniques. Carbonaceous films on iron substrates with thickness ranging from 60 nm to 110 nm were subjected to high fluence implantations with mass analyzed D +3 ions with energies ranging from 600 eV to 3000 eV and fluxes ranging from 5 × 10 14D/ cm2 s to 5 × 10 15D/ cm2 s, respectively. Deuterium re-emission upstream, deuterium permeation downstream and secondary ions sputtered from the implantation surface were measured as a function of implantation fluence for specimens at 420 K. The present studies indicate that the a-C : H film permeability is directly related to the time, hence the fluence, required to achieve isotopic replacement and saturation of the deuterium ion beam atoms stopped in the implant region. Once the deuterium saturation level is achieved in the layer, a significant fraction of the implanting ions can result in permeation. For the present experiment, this permeation factor was much higher than that for uncoated iron specimens subjected to similar beam conditions. Carbon sputter yields of 0.008-0.01 C/D were determined in this work for 1000-eV to 400-eV deuterium ions incident on a-C : H films.

  4. Penetration of radionuclides across the skin. Rat age dependent promethium permeation through skin in vitro

    International Nuclear Information System (INIS)

    Kassai, Z.; Kassai, A.; Bauerova, K.; Koprda, V.; Harangozo, M.; Bendova, P.; Bujnova, A.

    2003-01-01

    The composition and the permeation properties of the skin are dependent on age. In the animal models for permation studies, age affects the mechanical as well as the permeation properties significantly. The time dependence of permeation of 147 Pm 3+ from aqueous solution was established by the animal skin model and the age dependence of promethium permeation through the skin was examined. The aim was to find the optimum rat skin age model for radionuclide permeation studies and to assess the relative importance of the main permeation pathways: transepidermal and transfollicular permeation. The skin from 5-day-old rats (5DR) was found to represent the optimum animal model to study transepidermal permeation of ions. The skin from 9-day-old rats (9DR) was selected to study transfollicular permeation of ions. Comparison of the permeated amounts of promethium through the skin without hairs (3 DR to 6 DR) and with hairs (7DR to 12DR) showed that the additional permation mode via follicles significantly contributed to the permeation rate and extent. (author)

  5. Current Design of the Flange Type Hydrogen Permeation Sensor in Liquid Breeder

    International Nuclear Information System (INIS)

    Lee, E. H.; Jin, H. G.; Yoon, J. S.; Kim, S. K.; Lee, D. W.; Lee, H. G.

    2015-01-01

    In 2004, A. Ciampichetti et al. proposed a hollow capsule shape permeation sensor and they theoretically and experimentally evaluated the performance of the sensor made of Nb membrane at test condition of 500 .deg. C. However, the evaluation result showed the measured hydrogen permeation flux in the sensor much lower than the predicted one and they concluded that, the result is due to the formation of an oxide layer on the sensor membrane surface. Three years later, A. Ciampichetti et al. observed that a hollow capsule shape permeation sensor has too long response time to measure hydrogen concentration in liquid breeder. However, they suggested optimizing the sensor geometry with the reduction of the ratio 'total sensor volume/permeation surface' to overcome the low hydrogen permeating flux. For development of the liquid breeding technologies in nuclear fusion, the permeation sensor to measure tritium concentration in liquid metal breeder has been developed. Lee et al. proposed a flange type permeation sensor to dramatically reduce the ratio sensor 'inside volume/permeation surface' and to remove membrane welding during sensor manufacture process. However, the flange type sensor has problem with sealing. In present study, the modified flange sensor design with a metallic C-ring spring gasket is introduced. The modified sensor will be verified and evaluated under high temperature conditions by end of 2015

  6. Current Design of the Flange Type Hydrogen Permeation Sensor in Liquid Breeder

    Energy Technology Data Exchange (ETDEWEB)

    Lee, E. H.; Jin, H. G.; Yoon, J. S.; Kim, S. K.; Lee, D. W. [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of); Lee, H. G. [National Fusion Research Institute, Daejeon (Korea, Republic of)

    2015-10-15

    In 2004, A. Ciampichetti et al. proposed a hollow capsule shape permeation sensor and they theoretically and experimentally evaluated the performance of the sensor made of Nb membrane at test condition of 500 .deg. C. However, the evaluation result showed the measured hydrogen permeation flux in the sensor much lower than the predicted one and they concluded that, the result is due to the formation of an oxide layer on the sensor membrane surface. Three years later, A. Ciampichetti et al. observed that a hollow capsule shape permeation sensor has too long response time to measure hydrogen concentration in liquid breeder. However, they suggested optimizing the sensor geometry with the reduction of the ratio 'total sensor volume/permeation surface' to overcome the low hydrogen permeating flux. For development of the liquid breeding technologies in nuclear fusion, the permeation sensor to measure tritium concentration in liquid metal breeder has been developed. Lee et al. proposed a flange type permeation sensor to dramatically reduce the ratio sensor 'inside volume/permeation surface' and to remove membrane welding during sensor manufacture process. However, the flange type sensor has problem with sealing. In present study, the modified flange sensor design with a metallic C-ring spring gasket is introduced. The modified sensor will be verified and evaluated under high temperature conditions by end of 2015.

  7. Comparison on implantation-driven permeation characteristics of fusion reactor structural materials

    Energy Technology Data Exchange (ETDEWEB)

    Longhurst, G.R.; Anderl, R.A.; Struttmann, D.A. (Idaho National Engineering Lab., Idaho Falls)

    Implantation-driven permeation experiments have been conducted on samples of the ferritic steel HT-9, the austenitic Primary Candidate Alloy (PCA) and the vanadium alloy V-15Cr-5Ti using D{sub 3}{sup +} ions under conditions that simulate charge-exchange neutral loading on a fusion reactor first wall. The steels all exhibited an initially intense permeation spike followed by an exponential decrease to low steady-state values. That spike was not evident in the V-15Cr-5Ti experiments. Steady-state permeation was highest in the vanadium alloy and lowest in the austenitic steel. Though permeation rates in the HT-9 were lower than those in V-15Cr-5Ti, permeation transients were much faster in HT-9 than in other materials tested. Sputtering of the steel surface resulted in enhanced reemission, whereas in the vanadium tests, recombination and diffusivity both appeared to diminish as the deuterium concentration rose. We conclude that for conditions comparable to those of these experiments, tritium retention and permeation loss in first wall structures made of steels will be less than in structures made of V-15Cr-5Ti.

  8. The formation of tritium permeation barriers by CVD

    International Nuclear Information System (INIS)

    Forcey, K.S.; Perujo, A.; Reiter, F.; Lolli-Ceroni, P.L.

    1993-01-01

    The effectiveness as permeation barriers of the following CVD coatings have been investigated: TiC (1 to 2 μm in thickness); a bi-layer of TiN on TiC (3 μm total thickness) and CVD Al 2 O 3 on a TiN/TiC bi-layer. The substrate materials were TZM (a Mo alloy) and 316L stainless steel in the form of discs of diameter 48 mm and thickness 0.1 or 1 mm. Permeation measurements were performed in the temperature range 515-742 K using deuterium at pressures in the range 1-50 kPa. CVD layers were shown to form reasonably effective permeation barriers. At a temperature of 673 K TiC is around 6000 times less permeable to deuterium than 316L stainless steel. (orig.)

  9. Ion-driven deuterium permeation through tungsten at high temperatures

    Science.gov (United States)

    Gasparyan, Yu. M.; Golubeva, A. V.; Mayer, M.; Pisarev, A. A.; Roth, J.

    2009-06-01

    The ion-driven permeation (IDP) through 50 μm thick pure tungsten foils was measured in the temperature range of 823-923 K during irradiation by 200 eV/D + ion beam with a flux of 10 17-10 18 D/m 2s. Gas driven permeation (GDP) from the deuterium background gas was observed as well. Calculations using both the analytical formula for the diffusion limited regime (DLR) and the TMAP 7 code gave good agreement with the experimental data. Defects with a detrapping energy of (2.05 ± 0.15) eV were found to limit the permeation lag time in our experimental conditions.

  10. Ion-driven deuterium permeation through tungsten at high temperatures

    Energy Technology Data Exchange (ETDEWEB)

    Gasparyan, Yu.M., E-mail: yury.gasparyan@ipp.mpg.d [Max-Planck-Institut fuer Plasmaphysik, EURATOM Association, Boltzmanstrasse 2, D-85748 Garching (Germany); Moscow Engineering and Physics Institute, Kashirskoe sh. 31, Moscow 115409 (Russian Federation); Golubeva, A.V. [RRC ' Kurchatov Institute' , Ac. Kurchatov sq., 1/1, Moscow RU-123182 (Russian Federation); Mayer, M. [Max-Planck-Institut fuer Plasmaphysik, EURATOM Association, Boltzmanstrasse 2, D-85748 Garching (Germany); Pisarev, A.A. [Moscow Engineering and Physics Institute, Kashirskoe sh. 31, Moscow 115409 (Russian Federation); Roth, J. [Max-Planck-Institut fuer Plasmaphysik, EURATOM Association, Boltzmanstrasse 2, D-85748 Garching (Germany)

    2009-06-15

    The ion-driven permeation (IDP) through 50 mum thick pure tungsten foils was measured in the temperature range of 823-923 K during irradiation by 200 eV/D{sup +} ion beam with a flux of 10{sup 17}-10{sup 18} D/m{sup 2}s. Gas driven permeation (GDP) from the deuterium background gas was observed as well. Calculations using both the analytical formula for the diffusion limited regime (DLR) and the TMAP 7 code gave good agreement with the experimental data. Defects with a detrapping energy of (2.05 +- 0.15) eV were found to limit the permeation lag time in our experimental conditions.

  11. Ion-driven deuterium permeation through tungsten at high temperatures

    International Nuclear Information System (INIS)

    Gasparyan, Yu.M.; Golubeva, A.V.; Mayer, M.; Pisarev, A.A.; Roth, J.

    2009-01-01

    The ion-driven permeation (IDP) through 50 μm thick pure tungsten foils was measured in the temperature range of 823-923 K during irradiation by 200 eV/D + ion beam with a flux of 10 17 -10 18 D/m 2 s. Gas driven permeation (GDP) from the deuterium background gas was observed as well. Calculations using both the analytical formula for the diffusion limited regime (DLR) and the TMAP 7 code gave good agreement with the experimental data. Defects with a detrapping energy of (2.05 ± 0.15) eV were found to limit the permeation lag time in our experimental conditions.

  12. Purification Simulation With Vapor Permeation and Distillation-Adsorption In Bioethanol Plant

    Directory of Open Access Journals (Sweden)

    Misri Gozan

    2017-04-01

    Full Text Available High purity of Bioethanol is required in biofuel mixing with gasoline (EXX. In bioethanol production line, the azeotropic property of ethanol-water becomes the barrier for purification process. This study examined two bioethanol separation processes by support of simulation tools, Superpro Designer 9.0 software. Ethanol purity and a low costeconomical process were the major considerations. Purification method of vapor permeation membrane technology was compared with distillation-adsorption method. Data from previous lab experiments and some literatures were used. The results showed that distillation-adsorption method is more economical compared to vapor permeation technology. Payback period of the simulation is 3.9 years and 4.3 years to distillation adsorption and vapor permeation respectively with each IRR value is 20.23% and 17.89%. Initial investment value of vapor permeation is 9.6% higher than distillation method. Significant difference observed in operating costs, since more units involved in vapor permeation require more labors to operate.

  13. Recent developments in skin mimic systems to predict transdermal permeation.

    Science.gov (United States)

    Waters, Laura J

    2015-01-01

    In recent years there has been a drive to create experimental techniques that can facilitate the accurate and precise prediction of transdermal permeation without the use of in vivo studies. This review considers why permeation data is essential, provides a brief summary as to how skin acts as a natural barrier to permeation and discusses why in vivo studies are undesirable. This is followed by an in-depth discussion on the extensive range of alternative methods that have been developed in recent years. All of the major 'skin mimic systems' are considered including: in vitro models using synthetic membranes, mathematical models including quantitative structure-permeability relationships (QSPRs), human skin equivalents and chromatographic based methods. All of these model based systems are ideally trying to achieve the same end-point, namely a reliable in vitro-in vivo correlation, i.e. matching non-in vivo obtained data with that from human clinical trials. It is only by achieving this aim, that any new method of obtaining permeation data can be acknowledged as a potential replacement for animal studies, for the determination of transdermal permeation. In this review, the relevance and potential applicability of the various models systems will also be discussed.

  14. Importance of the Direct Contact of Amorphous Solid Particles with the Surface of Monolayers for the Transepithelial Permeation of Curcumin.

    Science.gov (United States)

    Kimura, Shunsuke; Kasatani, Sachiha; Tanaka, Megumi; Araki, Kaeko; Enomura, Masakazu; Moriyama, Kei; Inoue, Daisuke; Furubayashi, Tomoyuki; Tanaka, Akiko; Kusamori, Kosuke; Katsumi, Hidemasa; Sakane, Toshiyasu; Yamamoto, Akira

    2016-02-01

    The amorphization has been generally known to improve the absorption and permeation of poorly water-soluble drugs through the enhancement of the solubility. The present study focused on the direct contact of amorphous solid particles with the surface of the membrane using curcumin as a model for water-insoluble drugs. Amorphous nanoparticles of curcumin (ANC) were prepared with antisolvent crystallization method using a microreactor. The solubility of curcumin from ANC was two orders of magnitude higher than that of crystalline curcumin (CC). However, the permeation of curcumin from the saturated solution of ANC was negligible. The transepithelial permeation of curcumin from ANC suspension was significantly increased as compared to CC suspension, while the permeation was unlikely correlated with the solubility, and the increase in the permeation was dependent on the total concentration of curcumin in ANC suspension. The absorptive transport of curcumin (from apical to basal, A to B) from ANC suspension was much higher than the secretory transport (from basal to apical, B to A). In vitro transport of curcumin through air-interface monolayers is large from ANC but negligible from CC particles. These findings suggest that the direct contact of ANC with the absorptive membrane can play an important role in the transport of curcumin from ANC suspension. The results of the study suggest that amorphous particles may be directly involved in the transepithlial permeation of curcumin.

  15. SAMPA: A free software tool for skin and membrane permeation data analysis.

    Science.gov (United States)

    Bezrouk, Aleš; Fiala, Zdeněk; Kotingová, Lenka; Krulichová, Iva Selke; Kopečná, Monika; Vávrová, Kateřina

    2017-10-01

    Skin and membrane permeation experiments comprise an important step in the development of a transdermal or topical formulation or toxicological risk assessment. The standard method for analyzing these data relies on the linear part of a permeation profile. However, it is difficult to objectively determine when the profile becomes linear, or the experiment duration may be insufficient to reach a maximum or steady state. Here, we present a software tool for Skin And Membrane Permeation data Analysis, SAMPA, that is easy to use and overcomes several of these difficulties. The SAMPA method and software have been validated on in vitro and in vivo permeation data on human, pig and rat skin and model stratum corneum lipid membranes using compounds that range from highly lipophilic polycyclic aromatic hydrocarbons to highly hydrophilic antiviral drug, with and without two permeation enhancers. The SAMPA performance was compared with the standard method using a linear part of the permeation profile and a complex mathematical model. SAMPA is a user-friendly, open-source software tool for analyzing the data obtained from skin and membrane permeation experiments. It runs on a Microsoft Windows platform and is freely available as a Supporting file to this article. Copyright © 2017 Elsevier Ltd. All rights reserved.

  16. Tritium inventory and permeation in the ITER breeding blanket

    International Nuclear Information System (INIS)

    Violante, V.; Tosti, S.; Sibilia, C.; Felli, F.; Casadio, S.; Alvani, C.

    2000-01-01

    A model has allowed us to perform the analysis of the tritium inventory and permeation in the international thermonuclear experimental reactor (ITER) breeding blanket under the hypothesis of steady state conditions. Li 2 ZrO 3 (reference) and Li 2 TiO 3 (alternative) have been studied as breeding materials. The total breeder inventory assessed is 7.64 g for the Li 2 ZrO 3 at reference temperature. The model has also been used for a parametric analysis of the tritium permeation. At reference temperature and purge helium velocity of 0.01 m/s, the HT partial pressure is ranging from 10 to 30 Pa in the breeder and 1.5x10 -3 Pa in the beryllium. At 0.1 m/s of purge helium velocity, the HT partial pressure is reduced of one order by magnitude in the breeder and becomes 5x10 -5 Pa in the beryllium. The tritium permeation into the coolant for the whole blanket is ranging from 100 to 250 mCi per day for purge helium velocity of 0.01 m/s. The analysis of the tritium inventory and permeation for the alternative Li 2 TiO 3 breeding material has been carried out too. The tritium inventory in the breeder is in the range from 6 to 375 g larger than in Li 2 ZrO 3 by about a factor 5; the tritium permeation into coolant is comparable to the Li 2 ZrO 3 one. This analysis provides indications on the influence of the operating parameters on the tritium control in the ITER breeding blanket; particularly the control of the tritium inventory by the temperature and the tritium permeation by the purge gas velocity

  17. Changes on iron electrode surface during hydrogen permeation in borate buffer solution

    International Nuclear Information System (INIS)

    Modiano, S.; Carreno, J.A.V.; Fugivara, C.S.; Torresi, R.M.; Vivier, V.; Benedetti, A.V.; Mattos, O.R.

    2008-01-01

    Hydrogen interaction with oxide films grown on iron electrodes at open circuit potential (E oc ) and in the passive region (+0.30 V ECS ) was studied by chronopotentiometry, chronoamperometry and electrochemical impedance spectroscopy techniques. The results were obtained in deaerated 0.3 mol L -1 H 3 BO 3 + 0.075 mol L -1 Na 2 B 4 O 7 (BB, pH 8.4) solution before, during and after hydrogen permeation. The iron oxide film modification was also investigated by means of in situ X-ray absorption near-edge spectroscopy (XANES) and scanning electrochemical microscopy (SECM) before and during hydrogen permeation. The main conclusion was that the passive film is reduced during the hydrogen diffusion. The hydrogen permeation stabilizes the iron surface at a potential close to the thermodynamic water stability line where hydrogen evolution can occur. The stationary condition required for the determination of the permeation parameters cannot be easily attained on iron surface during hydrogen permeation. Moreover, additional attention must be paid when obtaining the transport parameters using the classical permeation cell

  18. Enzymatic hydrolysis of lactose of whey permeate

    Directory of Open Access Journals (Sweden)

    Karina Nascimento de Almeida

    2015-09-01

    Full Text Available The whey permeate is the residual of the concentration process of the whey proteins by ultrafiltration method. It contains important nutrients such as lactose, minerals and some proteins and lipids. It is without an ending industrial waste that causes serious damage to the environment. For its full use the lactose must be hydrolyzed to enable its consumption by intolerant people. The enzymatic hydrolysis by lactase (β-galactosidase of Kluyveromyces lactis yeast is a safe method that does not compromise the integrity of other nutrients, enabling further use of the permeate as a raw material. This study aimed to perform tests of enzymatic hydrolysis of lactose in whey permeate formulations in a concentration of 0.2%, 0.7% and 1% at 30, 60 and 90 minutes with pH 6.3 medium and 37 °C. The reactions were monitored by high performance liquid chromatography which showed that the enzyme concentration of 0.7% at time 30 minutes formulations became safe for consumption by lactose intolerant people, according to minimum levels established by law.

  19. Electrochemical measurement of tritium and hydrogen permeation through iron membranes

    International Nuclear Information System (INIS)

    Hagi, Hideki; Hayashi, Yasunori

    1988-01-01

    Permeation rates of tritium and hydrogen through iron were measured by the electrochemical method in which an aqueous solution containing 3.7 x 10 12 Bq/m 3 tritium was used as a cathodic electrolyte. Tritium and hydrogen were introduced from one side of a specimen by cathodic polarization with a constant current density, while at the other side of the specimen the permeated tritium and hydrogen were extracted by potentiostatical ionization. Nearly all of the potentiostatic current of the extraction side stands for the ionization of hydrogen, because the concentration of tritium in the cathodic electrolyte is very small. The amount of permeated hydrogen was obtained by integrating the anodic current, and that of tritium was determined by measuring the radioactivity of the electrolyte sampled from the extraction side. The separation factor for permeation obtained under steady state conditions (the ratio of permeation rates of hydrogen to tritium divided by the ratio of the concentration of hydrogen to tritium in the charging electrolyte) is 12 at 288 K. This value is independent of cathodic current density. Diffusion coefficients of tritium (D T ) and hydrogen (D H ) in iron were determined from the time lag of tritium and hydrogen permeation. For annealed specimens at 286 K, D T = 9 x 10 -10 m 2 /s and D H = 4 x 10 -9 m 2 /s, and for 9% cold-worked specimens at 284 K, D T = 3 x 10 -10 m 2 /s and D H = 4 x 10 -10 m 2 /s. (author)

  20. Re-evaluation of SiC permeation coefficients at high temperatures

    Energy Technology Data Exchange (ETDEWEB)

    Yamamoto, Yasushi, E-mail: yama3707@kansai-u.ac.jp [Faculty of Engineering Science, Kansai Univ., Yamate-cho, Suita, Osaka 564-8680 (Japan); Murakami, Yuichiro; Yamaguchi, Hirosato; Yamamoto, Takehiro; Yonetsu, Daigo [Faculty of Engineering Science, Kansai Univ., Yamate-cho, Suita, Osaka 564-8680 (Japan); Noborio, Kazuyuki [Hydrogen Isotope Research Center, Univ. of Toyama, Toyama, Toyama 930-8555 (Japan); Konishi, Satoshi [Institute of Advanced Energy, Kyoto Univ., Gokasho, Uji, Kyoto 611-0011 (Japan)

    2016-11-01

    Highlights: • The deuterium permeation coefficients of CVD-SiC at 600–950 °C were evaluated. • The wraparound flow was reduced to less than 1/100th of the permeation flow. • CVD-SiC materials are very effective as hydrogen isotope permeation barriers. - Abstract: Since 2007, our group has studied the deuterium permeation and diffusion coefficients for SiC materials at temperatures above 600 °C as a means of evaluating the tritium inventory and permeation in fusion blankets. During such measurements, control and evaluation of the wraparound flow through the sample holder are important, and so the heated sample holder is enclosed by a glass tube and kept under vacuum during experimental trials. However, detailed studies regarding the required degree of vacuum based on model calculations have shown that the wraparound flow is much larger than expected, and so can affect measurements at high temperatures. We therefore modified the measurement apparatus based on calculations involving reduced pressure in the glass tube, and are now confident that the measurement error is only several percent, even at 950 °C. In this paper, recent experimental results obtained with a chemical vapor deposition (CVD)-SiC sample over the temperature range of 600–950 °C are presented, showing that the permeation coefficient for CVD-SiC is more than three orders of magnitude smaller than that for stainless steel (SS316) at 600 °C, and that at 950 °C, the coefficient for CVD-SiC is almost equal to that for SUS316 at 550 °C.

  1. Alteration of skin hydration and its barrier function by vehicle and permeation enhancers: a study using TGA, FTIR, TEWL and drug permeation as markers.

    Science.gov (United States)

    Shah, D K; Khandavilli, S; Panchagnula, R

    2008-09-01

    Vehicles and permeation enhancers (PEs) used in transdermal drug delivery (TDD) of a drug can affect skin hydration, integrity and permeation of the solute administered. This investigation was designed to study the effect of the most commonly used vehicles and PEs on rat skin hydration, barrier function and permeation of an amphiphilic drug, imipramine hydrochloride (IMH). An array of well-established techniques were used to confirm the findings of the study. Thermogravimetric analysis (TGA) and Fourier transform infrared (FTIR) spectroscopy were used to determine changes in skin hydration. Alteration of the stratum corneum (SC) structure was investigated using FTIR studies. To monitor the barrier function alteration, transepidermal water loss (TEWL) measurement and permeation studies were performed. Our findings indicate that with hydration, there was an increase in the bound water content of the skin, and pseudoequilibrium of hydration (a drastic decrease in hydration rate) was achieved at around 12 h. Hydration increased the ratio between amide-I and amide-II peaks in FTIR and reduced the C-H stretching peak area. Both propylene glycol (PG) and ethanol (EtOH) dehydrated skin, with the latter showing a predominant effect. Furthermore, it was confirmed that PG and EtOH decreased the bound water content due to alteration in the protein domains and extraction of SC lipids, respectively. The effect of hydration on the SC was found to be similar to that reported for temperature. Permeation studies revealed that the dehydration caused by vehicles decreased IMH flux, whereas the flux was enhanced by PEs. The role of partition was predominant for the permeation of IMH through dehydrated skin. A synergistic effect was observed for PG and menthol in the enhancement of IMH. Further findings provided strong evidence that PG affects protein domains and EtOH extracts lipids from the bilayer. Both PG and EtOH, with or without PEs, increased TEWL. Initial TEWL was well

  2. Uses and evaluation methods of potential hydrogen permeation barriers for nuclear reactor materials

    International Nuclear Information System (INIS)

    Noga, J.O.; Piercy, G.R.; Bowker, J.T.

    1985-07-01

    This report summarizes results on the use of coatings as hydrogen permeation barriers on nuclear reactor component materials. Two classes of base materials were considered, exothermic hydrogen absorbers and endothermic hydrogen absorbers. The results of the tests indicate that substantial reductions in the amount of hydrogen absorbed by a metal can be achieved through the use of hydrogen permeation barrier coatings. Gold was determined to provide an effective hydrogen permeation barrier on Zr-2-1/2 Nb pressure tube material. Tin was determined to be a suitable hydrogen permeation barrier when applied on AISI 410 stainless steel and iron. Both gas phase and electrochemical permeation techniques were used to determine hydrogen permeabilities through coatings and base materials

  3. Drug Release and Skin Permeation from Lipid Liquid Crystalline Phases

    Science.gov (United States)

    Costa-Balogh, F. O.; Sparr, E.; Sousa, J. J. S.; Pais, A. A. C. C.

    We have studied drug release and skin permeation from several different liquid crystalline lipid formulations that may be used to control the respective release rates. We have studied the release and permeation through human skin of a water-soluble and amphiphilic drug, propranolol hydrochloride, from several formulations prepared with monoolein and phytantriol as permeation enhancers and controlled release excipients. Diolein and cineol were added to selected formulations. We observed that viscosity decreases with drug load, wich is compatible with the occurrence of phase changes. Diolein stabilizes the bicontinuous cubic phases leading to an increase in viscosity and sustained release of the drug. The slowest release was found for the cubic phases with higher viscosity. Studies on skin permeation showed that these latter formulations also presented lower permeability than the less viscous monoolein lamellar phases. Formulations containing cineol originated higher permeability with higher enhancement ratios. Thus, the various formulations are adapted to different circumstances and delivery routes. While a slow release is usually desired for drug sustained delivery, the transdermal route may require a faster release. Lamellar phases, which are less viscous, are more adapted to transdermal applications. Thus, systems involving lamellar phases of monoolein and cineol are good candidates to be used as skin permeation enhancers for propranolol hydrochloride.

  4. Implications of recent implantation-driven permeation experiments for fusion reactor safety

    International Nuclear Information System (INIS)

    Longhurst, G.R.; Anderl, R.A.; Struttmann, D.A.

    1986-01-01

    Metal structures exposed to the plasma in tritium-burning fusion reactors will be subject to implantation-driven permeation (IDP) of tritium. Permeation rates for IDP in fusion structural materials are usually high because the tritium atoms enter the material without having to go through the dissociation and solution steps required of tritium-bearing gas molecules. These surface processes, which may be rate limiting in PDP, actually enhance permeation in IDP by inhibiting the return of tritium to the plasma side of the structure. Experiments have been conducted at the Idaho National Engineering Laboratory (INEL) to investigate the nature of IDP by simulating conditions experienced by structures exposed to the plasma. These experiments have shown that surface conditions are important to tritium permeation in materials endothermic to hydrogen solution such as austenitic and ferritic steels. In reactive metals such as vanadium, surface processes appear to totally control the permeation. The purpose of this paper is to review the progress of those experiments and to discuss the implications that the results have regarding the tritium-related safety concerns of fusion reactors

  5. SC lipid model membranes designed for studying impact of ceramide species on drug diffusion and permeation--part II: diffusion and permeation of model drugs.

    Science.gov (United States)

    Ochalek, M; Podhaisky, H; Ruettinger, H-H; Wohlrab, J; Neubert, R H H

    2012-10-01

    The barrier function of two quaternary stratum corneum (SC) lipid model membranes, which were previously characterized with regard to the lipid organization, was investigated based on diffusion studies of model drugs with varying lipophilicities. Diffusion experiments of a hydrophilic drug, urea, and more lipophilic drugs than urea (i.e. caffeine, diclofenac sodium) were conducted using Franz-type diffusion cells. The amount of permeated drug was analyzed using either HPLC or CE technique. The subjects of interest in the present study were the investigation of the influence of physicochemical properties of model drugs on their diffusion and permeation through SC lipid model membranes, as well as the study of the impact of the constituents of these artificial systems (particularly ceramide species) on their barrier properties. The diffusion through both SC lipid model membranes and the human SC of the most hydrophilic model drug, urea, was faster than the permeation of the more lipophilic drugs. The slowest rate of permeation through SC lipid systems occurred in the case of caffeine. The composition of SC lipid model membranes has a significant impact on their barrier function. Model drugs diffused and permeated faster through Membrane II (presence of Cer [EOS]). In terms of the barrier properties, Membrane II is much more similar to the human SC than Membrane I. Copyright © 2012 Elsevier B.V. All rights reserved.

  6. Effect of permeation enhancers on the penetration mechanism of transfersomal gel of ketoconazole

    Directory of Open Access Journals (Sweden)

    Reshmy Rajan

    2012-01-01

    Full Text Available The aim of the present research work was to investigate the potential of transfersome formulations for transdermal delivery of Ketoconazole (KTZ. KTZ is a broad-spectrum antifungal agent that is active against a wide variety of fungi and yeasts. It is readily but incompletely absorbed after oral dosing and is highly variable. The transfersomes were formulated by lipid film hydration technique using Rotary vacuum Evaporator. The prepared transfersomes were converted into suitable gel formulation and is evaluated for their gel characteristics like pH, viscosity, spreadability, extrudability, homogeneity, drug content, etc. Suitable essential oils acting as natural permeation enhancers were added to the transfersomal formulation of KTZ for their release studies. Studies proved that addition of suitable permeation enhancers to the transfersomal formulation improved the release and permeation of KTZ, which showed that the permeation enhancers modify the barrier to penetration present in skin without itself undergoing any change. From the various essential oils which are used as permeation enhancers, the formulation containing Eucalyptus oil showed better in vitro release and permeation as compared with other formulations containing different permeation enhancers.

  7. Practical experience of backwashing with SWRO permeate for UF fouling control

    KAUST Repository

    Li, Sheng; Heijman, Sebastiaan G J; Verberk, J. Q J C; Amy, Gary L.; Van Dijk, Johannis C.

    2013-01-01

    Effectiveness of seawater reverse osmosis (SWRO) permeate backwash on fouling control of seawater ultrafiltration was investigated at a pilot scale. A standard membrane module was used in this pilot to represent full-scale desalination plants. Results of the pilot show a good reproducibility. When the UF permeate was used for backwash, the frequency of chemically enhanced backwash (CEB) was around once per day. However, results of the pilot show that SWRO permeate backwashing could significantly reduce the CEB frequency. © 2013 Desalination Publications.

  8. A comparison of implantation-driven permeation characteristics of fusion reactor structural materials

    Science.gov (United States)

    Longhurst, G. R.; Anderl, R. A.; Struttmann, D. A.

    1986-11-01

    Implantation-driven permeation experiments have been conducted on samples of the ferritic steel HT-9, the austenitic Primary Candidate Alloy (PCA) and the vanadium alloy V-15Cr-5Ti using D 3+ ions under conditions that simulate charge-exchange neutral loading on a fusion reactor first wall. The steels all exhibited an initially intense permeation "spike" followed by an exponential decrease to low steady-state values. That spike was not evident in the V-15Cr-5Ti experiments. Steady-state permeation was highest in the vanadium alloy and lowest in the austenitic steel. Though permeation rates in the HT-9 were lower than those in V-15Cr-5Ti, permeation transients were much faster in HT-9 than in other materials tested. Sputtering of the steel surface resulted in enhanced reemission, whereas in the vanadium tests, recombination and diffusivity both appeared to diminish as the deuterium concentration rose. We conclude that for conditions comparable to those of these experiments, tritium retention and permeation loss in first wall structures made of steels will be less than in structures made of V-15Cr-5Ti.

  9. Hydrogen permeation inhibition by zinc-nickel alloy plating on steel XC68

    International Nuclear Information System (INIS)

    El Hajjami, A.; Gigandet, M.P.; De Petris-Wery, M.; Catonne, J.C.; Duprat, J.J.; Thiery, L.; Raulin, F.; Starck, B.; Remy, P.

    2008-01-01

    The inhibition of hydrogen permeation and barrier effect by zinc-nickel plating was investigated using the Devanathan-Stachurski permeation technique. The hydrogen permeation and hydrogen diffusion for the zinc-nickel (12-15%) plating on steel XC68 is compared with zinc and nickel. Hydrogen permeation and hydrogen diffusion were followed as functions of time at current density applied (cathodic side) and potential permanent (anodic side). The hydrogen permeation inhibition for zinc-nickel is intermediate to that of nickel and zinc. This inhibition was due to nickel-rich layer effects at the Zn-Ni alloy/substrate interface, is shown by GDOES. Zinc-nickel plating inhibited the hydrogen diffusion greater as compared to zinc. This diffusion resistance was due to the barrier effect caused by the nickel which is present at the interface and transformed the hydrogen atomic to Ni 2 H compound, as shown by GIXRD.

  10. Hydrogen permeation inhibition by zinc-nickel alloy plating on steel XC68

    Energy Technology Data Exchange (ETDEWEB)

    El Hajjami, A. [Institut UTINAM, UMR CNRS 6213, Sonochimie et Reactivite des Surfaces, Universite de Franche-Comte, 16 route de Gray, 25030 Besancon Cedex (France); Coventya S.A.S., 51 rue Pierre, 92588 Clichy Cedex (France); Gigandet, M.P. [Institut UTINAM, UMR CNRS 6213, Sonochimie et Reactivite des Surfaces, Universite de Franche-Comte, 16 route de Gray, 25030 Besancon Cedex (France)], E-mail: marie-pierre.gigandet@univ-fcomte.fr; De Petris-Wery, M. [Institut Universitaire de Technologie d' Orsay, Universite Paris XI, Plateau de Moulon, 91400 Orsay (France); Catonne, J.C. [Professeur Honoraire du Conservatoire national des arts et metiers (CNAM), Paris (France); Duprat, J.J.; Thiery, L.; Raulin, F. [Coventya S.A.S., 51 rue Pierre, 92588 Clichy Cedex (France); Starck, B.; Remy, P. [Lisi Automotive, 28 faubourg de Belfort, BP 19, 90101 Delle Cedex (France)

    2008-12-30

    The inhibition of hydrogen permeation and barrier effect by zinc-nickel plating was investigated using the Devanathan-Stachurski permeation technique. The hydrogen permeation and hydrogen diffusion for the zinc-nickel (12-15%) plating on steel XC68 is compared with zinc and nickel. Hydrogen permeation and hydrogen diffusion were followed as functions of time at current density applied (cathodic side) and potential permanent (anodic side). The hydrogen permeation inhibition for zinc-nickel is intermediate to that of nickel and zinc. This inhibition was due to nickel-rich layer effects at the Zn-Ni alloy/substrate interface, is shown by GDOES. Zinc-nickel plating inhibited the hydrogen diffusion greater as compared to zinc. This diffusion resistance was due to the barrier effect caused by the nickel which is present at the interface and transformed the hydrogen atomic to Ni{sub 2}H compound, as shown by GIXRD.

  11. Permeated defect detecting test method and device in reactor

    International Nuclear Information System (INIS)

    Sakurai, Yoshishige.

    1996-01-01

    The present invention provides a method of and a device capable of performing a test for entire inner surfaces of the reactor upon periodical inspection of a BWR type reactor while sufficiently taking countermeasures for radiation rays into consideration. Namely, the present invention comprises following steps. (1) A provisional step for taking a shroud head of a reactor core shroud and incore structural components above and below the shroud out of the reactor, discharging reactor water and water tightly closing openings such as reactor wall perforation holes, (2) a pretreatment step for washing exposed inner surfaces of the reactor and peeling deteriorated materials, (3) a first drying step for drying portions washed and peeled in the step (2), (4) a permeation step for applying a permeation liquid of a defect detecting medium on the exposed inner surfaces of the reactor, (5) a permeation liquid removing step for removing the an excess permeation liquid in the step (4), (6) a second drying step for drying corresponding portions after performing the step (5), and (7) a flaw detecting step for optically observing the corresponding portions after performing the step (6) and detecting flaws. (I.S.)

  12. A study on reverse osmosis permeating treatment for yarn dyeing ...

    African Journals Online (AJOL)

    This paper presents a fuzzy linear regression model for estimation of reverse osmosis permeating parameters conditions. The proposed model can effectively take on non-crisp, fuzzy and crisp data. This study model used for estimation of reverse osmosis permeating parameters data from Tirupur examines the variables that ...

  13. Effect of Nutrient Formulations on Permeation of Proteins and Lipids ...

    African Journals Online (AJOL)

    Purpose: To investigate the effect of nutrient formulations on the permeation of proteins and lipids through porcine intestine in vitro. Method: In vitro permeation studies of proteins and lipids of two peptide-based formulations, composed of various compounds and sources of hydrolyzed protein was carried out, and compared ...

  14. Studies on Tasar Cocoon Cooking Using Permeation Method

    Science.gov (United States)

    Javali, Uday C.; Malali, Kiran B.; Ramya, H. G.; Naik, Subhas V.; Padaki, Naveen V.

    2018-02-01

    Cocoon cooking is an important process before reeling of tasar silk yarn. Cooking ensures loosening of the filaments in the tasar cocoons thereby easing the process of yarn withdrawal during reeling process. Tasar cocoons have very hard shell and hence these cocoons need chemical cooking process to loosen the silk filaments. Attempt has been made in this article to study the effect of using vacuum permeation chamber for tasar cocoon cooking in order to reduce the cooking time and improve the quality of tasar silk yarn. Vacuum assisted permeation cooking method has been studied in this article on tasar daba cocoons for cooking efficiency, deflossing and reelability. Its efficiency has been evaluated with respect to different cooking methods viz, traditional and open pan cooking methods. The tasar silk produced after reeling process has been tested for fineness, strength and cohesion properties. Results indicate that permeation method of tasar cooking ensures uniform cooking with higher efficiency along with better reeling performance and improved yarn properties.

  15. Development of tritium permeation barriers on Al base in Europe

    Science.gov (United States)

    Benamati, G.; Chabrol, C.; Perujo, A.; Rigal, E.; Glasbrenner, H.

    The development of the water cooled lithium lead (WCLL) DEMO fusion reactor requires the production of a material capable of acting as a tritium permeation barrier (TPB). In the DEMO blanket reactor permeation barriers on the structural material are required to reduce the tritium permeation from the Pb-17Li or the plasma into the cooling water to acceptable levels (HIP) technology and spray (this one developed also for repair) deposition techniques. The final goal is to select a reference technique to be used in the blanket of the DEMO reactor and in the ITER test module fabrication. The activities performed in four European laboratories are summarised here.

  16. Tritium permeation in stainless-steel structures exposed to plasma ions

    International Nuclear Information System (INIS)

    Anderl, R.A.; Holland, D.F.; Struttmann, D.A.; Longhurst, G.R.; Merrill, B.J.

    1985-01-01

    This paper presents a comparison of the hydrogen permeation properties of the austenitic primary candidate alloy, PCA, and of the advanced ferritic alloy, HT-9. The comparison is based on experimental measurements of the permeation and reemission of deuterium from specimens undergoing implantation with 3-keV, D 3 + ions produced by an accelerator. Characterization of the specimen surface facing the ion beam is provided by secondary ion mass spectrometer (SIMS) analysis of the species sputtered from the surface during the implantation. Recombination and diffusivity data for PCA and HT-9 were derived from an analysis in which model calculations were applied to the reemission and permeation measurements

  17. Tritium permeation and recovery for the Flibe/He blanket design

    International Nuclear Information System (INIS)

    Moir, R.

    1984-10-01

    This study assumes tritium to be a gas dissolved in molten salt, with TF formation suppressed. Tritium permeates readily through the hot steel tubes of the reactor and steam generator and will leak into the steam system at the rate of about one gram per day in the absence of special permeation barriers, assuming that 1% of the helium coolant flow rate is processed for tritium recovery at 90% efficiency per pass. Tritiated water in the steam system is a personnel hazard at concentration levels well below one part per million and this level would soon be reached without costly isotopic processing. Alternatively, including a combination of permeation barriers on reactor and steam generator tubes and molten salt processing is estimated to reduce the leak rate into the steam system by over two orders of magnitude. For the option with the lowest estimated leak rate, 55 Ci/d, it may be possible to purge the steam system continuously to prevent tritiated water buildup. At best, isotopic separation of dilute tritiated water may not be necessary and for higher leak-rate options the isotopic processing rate can be reduced. The proposed permeation barrier for the reactor tubes is a 10 μm layer of tungsten which, in principle, will reduce tritium blanket permeation by a factor of about 300 below the bare-steel rate

  18. Permeation of cytotoxic formulations through swatches from selected medical gloves.

    Science.gov (United States)

    Klein, Michael; Lambov, Nikolai; Samev, Nikola; Carstens, Gerhard

    2003-05-15

    The permeability of selected medical glove materials to various cytotoxic agents is described. Fifteen cytotoxic agents were prepared at the highest concentrations normally encountered by hospital personnel. Four single-layer and two double-layer glove systems made of two materials--latex and neoprene--were exposed to the drugs for 30, 60, 90, 120, 150, and 180 minutes. Circular sections of the glove material were cut from the cuff and evaluated without any pretreatment. Permeability tests were conducted in an apparatus consisting of a donor chamber containing the cytotoxic solution and a collection chamber filled with water (the acceptor medium). The two sections were separated by the glove material. Permeating portions, collected in water as the acceptor medium, were analyzed by either ultraviolet-visible light spectrophotometry or high-performance liquid chromatography (HPLC). Permeation rates were calculated on the basis of the concentration of the cytotoxic agent in the acceptor medium. Spectrophotometric measurements were taken every 30 minutes, and HPLC analysis was performed at the end of the three-hour period. Average permeation rates for 14 drugs were low (materials. All glove materials tested were impermeable to most of the cytotoxic agents over a period of three hours. Carmustine was the only agent that substantially permeated single-layer latex glove materials. Permeation of most tested cytotoxic formulations was low through swatches of material from various medical gloves.

  19. Expansion of thermodynamic model of solute permeation through reverse osmosis membrane

    International Nuclear Information System (INIS)

    Nishimaki, Kenzo; Koyama, Akio

    1994-01-01

    Many studies have been performed on permeation mechanism of solute and solvent in membrane separation process like reverse osmosis or ultrafiltration, and several models of solute/solvent permeation through membrane are proposed. Among these models, Kedem and Katchalsky, based on the theory of thermodynamics of irreversible processes, formulated the one-solute permeation process in their mathematical model, which treats membrane as a black box, not giving consideration to membrane structure and to interaction between membrane material and permeates, viz. solute and solvent. According to this theory, the driving force of solute/solvent permeation through membrane is the difference of their chemical potential between both sides of membrane, and the linear phenomenological equation is applied to describing the relation between driving force and flux of solute/solvent. This equation can be applied to the irreversible process only when the process is almost in equilibrium. This condition is supposed to be satisfied in the solute/solvent permeation process through compact membrane with fine pores like reverse osmosis membrane. When reverse osmosis is applied to treatment process for liquid waste, which usually contains a lot of solutes as contaminants, we can not predict the behavior of contaminants by the above one-solute process model. In the case of multi-solutes permeation process for liquid waste, the number of parameter in thermodynamic model increases rapidly with the number of solute, because of coupling phenomenon among solutes. In this study, we expanded the above thermodynamic model to multi-solute process applying operational calculus to the differential equations which describe the irreversible process of the system, and expressed concisely solute concentration vector as a matrix product. In this way, we predict the behavior of solutes in multi-solutes process, using values of parameters obtained in two-solutes process. (author)

  20. Oxygen permeation through oxygen ion oxide-noble metal dual phase composites

    NARCIS (Netherlands)

    Chen, C.S.; Chen, C.S.; Kruidhof, H.; Bouwmeester, Henricus J.M.; Verweij, H.; Burggraaf, Anthonie; Burggraaf, A.J.

    1996-01-01

    Oxygen permeation behaviour of three composites, yttria-stabilized zirconia-palladium, erbia-stabilized bismuth oxidenoble metal (silver, gold) was studied. Oxygen permeation measurements were performed under controlled oxygen pressure gradients at elevated temperatures. Air was supplied at one side

  1. Formation of permeation barriers on ceramic SiC/SiC composites

    International Nuclear Information System (INIS)

    Racault, C.; Fenici, P.

    1996-01-01

    The effectiveness as permeation barriers of the following CVD and PVD (sputtering) coatings has been investigated: TiC+Al 2 O 3 (CVD), SiC(CVD), SiO 2 (CVD), TiN(CVD), TiN(CVD)+TiN(PVD) and SiC(CVD)+Al 2 O 3 (PVD). The substrate material was a SiC/SiC composite, proposed as low activation structural material for fusion applications. Permeation measurements were performed in the temperature range 300-750 K using deuterium at pressures in the range 0.5-150 kPa. A linear dependence of permeation rate on pressure was measured. The efficiency of the coatings as deuterium permeation barriers is discussed in terms of coating microstructure. The best result was obtained with a bilayer of TiN(CVD) (15 μm) +TiN(PVD) (8 μm). (orig.)

  2. Final report for Allied-Signal Aerospace Company, Kansas City Division on protective glove permeation analysis

    International Nuclear Information System (INIS)

    Swearengen, P.M.; Johnson, J.S.; Priante, S.J.

    1990-01-01

    We conducted 25 separate glove fabric permeation studies during this project. The permeations were carried out in the small (1 inch) glass ASTM cell. One other permeation study was carded out with a large (two inch) modified ASTM cell for comparison with the small cell results. We also compared the LLNL procedure from both large and small cells with the standard ASTM test procedure which uses a liquid solution on the breakthrough side of the fabric (the liquid is then sampled on a periodic basis). In all comparisons we observed a close-correlation in breakthrough times between the two procedures and the two cell sizes. In the course of this study, we tested ten different glove materials. These included neoprene (original ASTM round-robin sheet stock, 16 mil thickness), Edmont Sol-Vex (nitrile), Pioneer nitrile, Pioneer Pylox (polyvinyl chloride), North Viton (trademark for fluoroelastomers), North SilverShield (Norfoil, trademark for a flexible metallized laminate), Safety 4 4H (patented plastic laminate), and QRP PolyTuff (polyurethane) 20G-2000 (5 mil), 23G-2300 (1.5 mil), and 25G-2500 (1.5 mil). Three of the glove materials, Viton and Silver Shield (North), and 4H (Safety 4), did not allow any permeation measurable by our system to either 1,2-dichloroethane or 3% diphenylmercury (in 1,2-dichloroethane) for a period greater than six hours. A fourth material, QRP Poly Tuff 2OG-2000, did not allow any measurable penetration of Asilamine (an aromatic diamine) for a time pedod of greater than 4 hours. Breakthrough times and curves were obtained for all other tested materials. Eleven different chemicals were used to challenge the glove materials. These included acetone, Asilamine, 1,2-dichloroethane, dichloromethane, isopropyl alcohol, a mixture of 3% diphenylmercury in 1,2-dichloroethane (w/w), phenol, and lso Verre Stripper, 4,4'-methylenedianil (MDA), 1,3-phenylenediamine (MPDA), and Shell Epon (R) curing agent Z

  3. Vapour permeation for the recovery of organic solvents from waste air streams: separation capacities and process optimization

    NARCIS (Netherlands)

    Leemann, M.; Leemann, M.; Eigenberger, G.; Strathmann, H.

    1996-01-01

    Vapour permeation is a potentially suitable technology for the recovery of organic solvents from waste air streams. New solvent stable capillary membrane modules that are currently emerging on the market provide large membrane areas for an acceptable price and enhance the competitiveness of this

  4. 40 CFR 1060.521 - How do I test fuel caps for permeation emissions?

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 32 2010-07-01 2010-07-01 false How do I test fuel caps for permeation... EQUIPMENT Test Procedures § 1060.521 How do I test fuel caps for permeation emissions? If you measure a fuel tank's permeation emissions with a nonpermeable covering in place of the fuel cap under § 1060.520(b)(5...

  5. Permeation of deuterium implanted into V-15Cr-5Ti

    International Nuclear Information System (INIS)

    Anderl, R.A.; Longhurst, G.R.; Struttmann, D.A.

    1987-01-01

    Permeation and reemission of deuterium for the vanadium alloy, V-15Cr-5Ti, was investigated using 3 keV, D 3 + ion beams from a small accelerator. The experiments consisted of measurement of the deuterium reemission and permeation rates as a function of implantation fluence for 0.5 mm thick specimens heated to temperatures from 623 K to 823 K. Implantation-side surface characterization was made by simultaneous measurements of sputtered ions with a secondary ion mass spectrometer (SIMS). For the experimental conditions used, the steady-state deuterium permeation flux in V-15Cr-5Ti is approximately 18% of the implantation flux. This is approximately 1000 times that seen in the austenitic stainless steel, PCA, and 200 times that seen in the ferritic steel, HT-9, under comparable conditions. Measurement of deuterium diffusivity in V-15Cr-5Ti using permeation break-through times indicates that D = 1.4x10 -8 exp(-0.11 eV/kT)(m 2 /s), over the temperature range 723 K to 823 K. (orig.)

  6. Permeation of deuterium implanted into V-15Cr-5Ti

    Science.gov (United States)

    Anderl, R. A.; Longhurst, G. R.; Struttmann, D. A.

    1987-02-01

    Permeation and reemission of deuterium for the vanadium alloy, V-15Cr-5Ti, was investigated using 3 keV, D 3+ ion beams from a small accelerator. The experiments consisted of measurement of the deuterium reemission and permeation rates as a function of implantation fluence for 0.5 mm thick specimens heated to temperatures from 623 K to 823 K. Implantation-side surface characterization was made by simultaneous measurements of sputtered ions with a secondary ion mass spectrometer (SIMS). For the experimental conditions used, the steady-state deuterium permeation flux in V-15Cr-5Ti is approximately 18% of the implantation flux. This is approximately 1000 times that seen in the austenitic stainless steel, PCA, and 200 times that seen in the ferritic steel, HT-9, under comparable conditions. Measurement of deuterium diffusivity in V-15Cr-5Ti using permeation break-through times indicates that D = 1.4 × 10 -8 exp( -0.11 eV/ kT) (m 2/s), over the temperature range 723 K to 823 K.

  7. Permeation of deuterium implanted into V-15Cr-5Ti

    Energy Technology Data Exchange (ETDEWEB)

    Anderl, R.A.; Longhurst, G.R.; Struttmann, D.A.

    1987-02-01

    Permeation and reemission of deuterium for the vanadium alloy, V-15Cr-5Ti, was investigated using 3 keV, D/sub 3//sup +/ ion beams from a small accelerator. The experiments consisted of measurement of the deuterium reemission and permeation rates as a function of implantation fluence for 0.5 mm thick specimens heated to temperatures from 623 K to 823 K. Implantation-side surface characterization was made by simultaneous measurements of sputtered ions with a secondary ion mass spectrometer (SIMS). For the experimental conditions used, the steady-state deuterium permeation flux in V-15Cr-5Ti is approximately 18% of the implantation flux. This is approximately 1000 times that seen in the austenitic stainless steel, PCA, and 200 times that seen in the ferritic steel, HT-9, under comparable conditions. Measurement of deuterium diffusivity in V-15Cr-5Ti using permeation break-through times indicates that D = 1.4x10/sup -8/ exp(-0.11 eVkT)(m/sup 2/s), over the temperature range 723 K to 823 K.

  8. The formation of hydrogen permeation barriers on steels by aluminising

    Science.gov (United States)

    Forcey, K. S.; Ross, D. K.; Wu, C. H.

    1991-06-01

    An extensive investigation has been carried out into the effectiveness of aluminised layers as permeation barriers on AISI 316L stainless and DIN 1.4914 martensitic steels. The study involved measurement of the hydrogen permeation rate through commercially aluminised steel discs of thicknesses in the range 1-1.6 mm, at temperatures between 250 and 600 °C and for an upstream hydrogen pressure of 10 5 Pa. The composition and structure of the aluminide layers were investigated by a number of techniques such as SEM, electron beam microprobe and X-ray diffraction. Accelerator based techniques such as RBS and NRA were employed to study the top micron or so of the surfaces of the samples. By these techniques it was found that the effectiveness of the permeation barrier depended on the formation of a thin surface oxide layer consisting of Al 2O 3. It was found that the permeation rate through the aluminised steels could be reduced by 3-4 orders of magnitude by forming a surface oxide layer up to a micron or so in thickness.

  9. Mechanisms of oxygen permeation through plastic films and barrier coatings

    Science.gov (United States)

    Wilski, Stefan; Wipperfürth, Jens; Jaritz, Montgomery; Kirchheim, Dennis; Mitschker, Felix; Awakowicz, Peter; Dahlmann, Rainer; Hopmann, Christian

    2017-10-01

    Oxygen and water vapour permeation through plastic films in food packaging or other applications with high demands on permeation are prevented by inorganic barrier films. Most of the permeation occurs through small defects (visualized by etching with reactive oxygen in a capacitively coupled plasma and subsequent SEM imaging. In this work, defects in SiO x -coatings deposited by plasma-enhanced chemical vapour deposition on polyethylene terephthalate (PET) are investigated and the mass transport through the polymer is simulated in a 3D approach. Calculations of single defects showed that there is no linear correlation between the defect area and the resulting permeability. The influence of adjacent defects in different distances was observed and led to flow reduction functions depending on the defect spacing and defect area. A critical defect spacing where no interaction between defects occurs was found and compared to other findings. According to the superposition principle, the permeability of single defects was added up and compared to experimentally determined oxygen permeation. The results showed the same trend of decreasing permeability with decreasing defect densities.

  10. Preventing method and device for underground permeation of hazardous material

    International Nuclear Information System (INIS)

    Funabashi, Kiyomi; Kurokawa, Hideaki; Fukazawa, Tetsuo; Yamazaki, Tadashi.

    1996-01-01

    In a method of preventing hazardous materials from permeating into ground by burying adsorbing materials underground, a plurality of adsorbing layers are laminated being spaced apart from each other, the concentration of the hazardous materials between each of the adsorbent layers is measured. When the concentration reaches a predetermined value, the adsorbent layers are regenerated. A suppression means for preventing hazardous materials from permeating into the ground are formed by an upper adsorbent layer and a lower adsorbent layer, and a means for measuring the concentration of hazardous materials passing through the upper adsorbent layer and a means for charging and discharging regenerated liquid are disposed. When it is detected that the poisonous materials can not be eliminated, the poisonous materials are already permeated to the adsorbent layer, and they start to inflow into underground water. In order to prevent it, an adsorbent layer is additionally disposed at the lower side of the place of detection to eliminate the poisonous materials completely thereby enabling to prevent poisonous materials from permeating into underground for a long period of time. (T.M.)

  11. Deuterium permeation measurements on tungsten using ion-beam-based detection

    Energy Technology Data Exchange (ETDEWEB)

    Kapser, Stefan [Max-Planck-Institut fuer Plasmaphysik, Boltzmannstr. 2, 85748 Garching (Germany); Physik-Department, Technische Universitaet Muenchen, James-Franck-Str. 1, 85748 Garching (Germany); Manhard, Armin; Toussaint, Udo von [Max-Planck-Institut fuer Plasmaphysik, Boltzmannstr. 2, 85748 Garching (Germany)

    2016-07-01

    Tungsten (W) is promising for the inner wall of a future fusion reactor, where it will be exposed to high fluxes of hydrogen (H) isotopes. Knowledge of their diffusion in W is important for safety and economic considerations, particularly concerning tritium. A common method to investigate H diffusion in metals are permeation experiments. Typically, gas loading and mass-spectrometric detection are used. Information about the diffusion can be gained from the temporal evolution of the permeation flux, whose magnitude is determined by the permeability (product of diffusivity and solubility). However, for low-permeability metals, the permeation flux can be unmeasurably small. For W this is the case near room temperature. We present a method that circumvents this problem. It is an improved version of experiments on nickel and stainless steel. The W is exposed to deuterium (D) plasma on one side and the permeating D is accumulated in a getter on the other side. A cover prevents D gettering from the gas phase. The amount in the getter is analysed by the nuclear reaction D({sup 3}He,p){sup 4}He.

  12. Permeation Barrier Coatings for the Helical Heat Exchanger

    International Nuclear Information System (INIS)

    Korinko, P.S.

    1999-01-01

    A permeation barrier coating was specified for the Helical Heat Exchanger (HHE) to minimize contamination through emissions and/or permeation into the nitrogen system for ALARA reasons. Due to the geometry of the HHE, a special coating practice was needed since the conventional method of high temperature pack aluminization was intractable. A survey of many coating companies was undertaken; their coating capabilities and technologies were assessed and compared to WSRC needs. The processes and limitations to coating the HHE are described. Slurry coating appears to be the most technically sound approach for coating the HHE

  13. Permeation of "Hydromer" Film: An Elastomeric Hydrogen-Capturing Biopolymer.

    Energy Technology Data Exchange (ETDEWEB)

    Karnesky, Richard A. [Sandia National Lab. (SNL-CA), Livermore, CA (United States); Friddle, Raymond William [Sandia National Lab. (SNL-CA), Livermore, CA (United States); Whaley, Josh A. [Sandia National Lab. (SNL-CA), Livermore, CA (United States); Smith, Geoffrey [New Mexico State Univ., Las Cruces, NM (United States)

    2015-12-01

    This report analyzes the permeation resistance of a novel and proprietary polymer coating for hydrogen isotope resistance that was developed by New Mexico State University. Thermal gravimetric analysis and thermal desoprtion spectroscopy show the polymer is stable thermally to approximately 250 deg C. Deuterium gas-driven permeation experiments were conducted at Sandia to explore early evidence (obtained using Brunauer - Emmett - Teller) of the polymer's strong resistance to hydrogen. With a relatively small amount of the polymer in solution (0.15%), a decrease in diffusion by a factor of 2 is observed at 100 and 150 deg C. While there was very little reduction in permeability, the preliminary findings reported here are meant to demonstrate the sensitivity of Sandia's permeation measurements and are intended to motivate the future exploration of thicker barriers with greater polymer coverage.

  14. Computational investigation of the effects of barrier layers on the permeation of hydrogen through metals

    International Nuclear Information System (INIS)

    Perkins, W.G.

    1975-01-01

    Results of a computational investigation of the permeation behavior of oxide-coated metal membranes are presented. A steady-state permeation model was developed which promises to be useful in evaluation of oxide layers on metals as hydrogen permeation barriers. The pressure and thickness dependence of steady state permeation through oxide-coated metal membranes is described using plots of logarithmic functions. (U.S.)

  15. Determination of solvents permeating through chemical protective clothing with a microsensor array.

    Science.gov (United States)

    Park, J; Zellers, E T

    2000-08-01

    The performance of a novel prototype instrument in determining solvents and solvent mixtures permeating through samples of chemical protective clothing (CPC) materials was evaluated. The instrument contains a mini-preconcentrator and an array of three polymer-coated surface-acoustic-wave (SAW) microsensors whose collective response patterns are used to discriminate among multiple permeants. Permeation tests were performed with a 2.54 cm diameter test cell in an open-loop configuration on samples of common glove materials challenged with four individual solvents, three binary mixtures, and two ternary mixtures. Breakthrough times, defined as the times required for the permeation rate to reach a value of 1 microg cm(-2) min(-1), determined by the instrument were within 3 min of those determined in parallel by manual sampling and gas chromatographic analysis. Permeating solvents were recognized (identified) from their response patterns in 59 out of 64 measurements (92%) and their vapor concentrations were quantified to an accuracy of +/- 31% (typically +/- 10%). These results demonstrate the potential for such instrumentation to provide semi-automated field or bench-top screening of CPC permeation resistance.

  16. Impacts of chemical enhancers on skin permeation and deposition of terbinafine.

    Science.gov (United States)

    Erdal, Meryem Sedef; Peköz, Ayca Yıldız; Aksu, Buket; Araman, Ahmet

    2014-08-01

    The addition of chemical enhancers into formulations is the most commonly employed approach to overcome the skin barrier. The objective of this work was to evaluate the effect of vehicle and chemical enhancers on the skin permeation and accumulation of terbinafine, an allylamine antifungal drug. Terbinafine (1% w/w) was formulated as a Carbopol 934 P gel formulation in presence and absence of three chemical enhancers, nerolidol, dl-limonene and urea. Terbinafine distribution and deposition in stratum corneum (SC) and skin following 8-h ex vivo permeation study was determined using a sequential tape stripping procedure. The conformational order of SC lipids was investigated by ATR-FTIR spectroscopy. Nerolidol containing gel formulation produced significantly higher enhancement in terbinafine permeation through skin and its skin accumulation was increased. ATR-FTIR results showed enhancer induced lipid bilayer disruption in SC. Urea resulted in enhanced permeation of terbinafine across the skin and a balanced distribution to the SC was achieved. But, dl-limonene could not minimize the accumulation of terbinafine in the upper SC. Nerolidol dramatically improved the skin permeation and deposition of terbinafine in the skin that might help to optimize targeting of the drug to the epidermal sites as required for both of superficial and deep cutaneous fungal infections.

  17. Comparison of implantation-driven permeation characteristics of fusion reactor structural materials

    International Nuclear Information System (INIS)

    Longhurst, G.R.; Anderl, R.A.; Struttmann, D.A.

    1986-01-01

    Implantation-driven permeation experiments have been conducted on samples of the ferritic steel HT-9, the austenitic Primary Candidate Alloy (PCA) and the vanadium alloy V-15Cr-5Ti using D 3 + ions under conditions that simulate charge-exchange neutral loading on a fusion reactor first wall. The steels all exhibited an initially intense permeation ''spike'' followed by an exponential decrease to low steady-state values. That spike was not evident in the V-15Cr-5Ti experiments. Steady-state permeation was highest in the vanadium alloy and lowest in the austenitic steel. Though permeation rates in the HT-9 were lower than those in V-15Cr-5Ti, permeation transients were much faster in HT-9 than in other materials tested. Ion-beam sputtering of the surface in the steel experiments resulted in enhanced remission at the front surface, whereas in the vanadium tests, recombination and diffusivity both appeared to diminish as the deuterium concentration rose. This may be due to a phase change in the material. We conclude that for conditions comparable to those of these experiments, tritium retention and loss in first wall structures made of steels will be less than in structures made of V-15Cr-5Ti

  18. Hydrogen permeation modification of 4140 steel by ion nitriding with pulsed plasmas

    Energy Technology Data Exchange (ETDEWEB)

    Bruzzoni, P.; Ortiz, M. [Comision Nacional de Energia Atomica, Buenos Aires (Argentina); Bruehl, S.P.; Gomez, B.J.A.; Feugeas, J.N. [Inst. de Fisica Rosario (UNR-CONICET), Rosario (Argentina); Nosei, L. [Inst. de Mecanica Aplicada y Estructuras (UNR), Rosario (Argentina)

    1998-11-10

    It is widely known that the hydrogen in steel produces embrittlement. This effect may cause the failure of the elements (confining walls, mechanical parts, etc.) whose surfaces are in contact with this gas or with processes in which hydrogen is continuously generated. In this work it is shown that the ion nitriding of the surface of AISI 4140 is a good mechanism to act as a barrier against hydrogen permeation in its bulk. The ion nitriding was performed using a square wave DC glow discharge. The development of a compound layer of iron nitrides was observed as the cause of the hydrogen permeation reduction. For equal duration of treatment, thicker compound layers were developed in higher discharge/post-discharge ratios in the square wave of the applied voltage onto the sample (cathode), with a greater reduction of hydrogen permeation coefficient as a consequence. Nevertheless, the permeation was not reduced to zero in any of the treatment conditions used. The results of the analysis of the permeation tests and the image of the photomicrographs showed that the existence of cracks, fractures, failures, etc. in the compound layer (pre-existing in the AISI 4140 steel) could be the cause of the residual hydrogen permeation. This can be attributed to the movement of the hydrogen through these defects diffusing through the original {alpha}-Fe phase of the non-treated steel. (orig.) 11 refs.

  19. In vitro-in vivo correlation in skin permeation.

    Science.gov (United States)

    Mohammed, D; Matts, P J; Hadgraft, J; Lane, M E

    2014-02-01

    In vitro skin permeation studies have been used extensively in the development and optimisation of delivery of actives in vivo. However, there are few reported correlations of such in vitro studies with in vivo data. The aim of this study was to investigate the skin permeation of a model active, niacinamide, both in vitro and in vivo. Conventional diffusion cell studies were conducted in human skin to determine niacinamide permeation from a range of vehicles which included dimethyl isosorbide (DMI), propylene glycol (PG), propylene glycol monolaurate (PGML), N-methyl 2-pyrrolidone (NMP), Miglyol 812N® (MG), and mineral oil (MO). Single, binary or ternary systems were examined. The same vehicles were subsequently examined to investigate niacinamide delivery in vivo. For this proof-of-concept study one donor was used for the in vitro studies and one volunteer for the in vivo investigations to minimise biovariability. Analysis of in vitro samples was conducted using HPLC and in vivo uptake of niacinamide was evaluated using Confocal Raman spectroscopy (CRS). The amount of niacinamide permeated through skin in vitro was linearly proportional to the intensity of the niacinamide signal determined in the stratum corneum in vivo. A good correlation was observed between the signal intensities of selected vehicles and niacinamide signal intensity. The findings provide further support for the use of CRS to monitor drug delivery into and across the skin. In addition, the results highlight the critical role of the vehicle and its disposition in skin for effective dermal delivery.

  20. Development of a plasma driven permeation experiment for TPE

    Energy Technology Data Exchange (ETDEWEB)

    Buchenauer, Dean, E-mail: dabuche@sandia.gov [Sandia National Laboratories, Livermore, CA (United States); Kolasinski, Robert [Sandia National Laboratories, Livermore, CA (United States); Shimada, Masa [Idaho National Laboratory, Idaho Falls, ID (United States); Donovan, David [Sandia National Laboratories, Livermore, CA (United States); Youchison, Dennis [Sandia National Laboratories, Albuquerque, NM (United States); Merrill, Brad [Idaho National Laboratory, Idaho Falls, ID (United States)

    2014-10-15

    Highlights: • We have designed and fabricated a novel tritium permeation membrane holder for use in the Tritium Plasma Experiment (TPE). • The membrane temperature is controlled by varying the cooling flow rate and proximity of a spiral cooling channel. • Sealing tests have demonstrated adequate helium leak rates up to temperatures of 1000 °C. • Flow modeling indicates a minimal helium pressure drop across the membrane holder (<700 Pa). • Thermal modeling shows good heat removal and minimal membrane temperature variation (±2%) even up to peak TPE ion fluxes. - Abstract: Experiments on retention of hydrogen isotopes (including tritium) at temperatures less than 800 °C have been carried out in the Tritium Plasma Experiment (TPE) at Idaho National Laboratory [1,2]. To provide a direct measurement of plasma driven permeation in plasma facing materials at temperatures reaching 1000 °C, a new TPE membrane holder has been built to hold test specimens (≤1 mm in thickness) at high temperature while measuring tritium permeating through the membrane from the plasma facing side. This measurement is accomplished by employing a carrier gas that transports the permeating tritium from the backside of the membrane to ion chambers giving a direct measurement of the plasma driven tritium permeation rate. Isolation of the membrane cooling and sweep gases from TPE's vacuum chamber has been demonstrated by sealing tests performed up to 1000 °C of a membrane holder design that provides easy change out of membrane specimens between tests. Simulations of the helium carrier gas which transports tritium to the ion chamber indicate a very small pressure drop (∼700 Pa) with good flow uniformity (at 1000 sccm). Thermal transport simulations indicate that temperatures up to 1000 °C are expected at the highest TPE fluxes.

  1. Development of a plasma driven permeation experiment for TPE

    International Nuclear Information System (INIS)

    Buchenauer, Dean; Kolasinski, Robert; Shimada, Masa; Donovan, David; Youchison, Dennis; Merrill, Brad

    2014-01-01

    Highlights: • We have designed and fabricated a novel tritium permeation membrane holder for use in the Tritium Plasma Experiment (TPE). • The membrane temperature is controlled by varying the cooling flow rate and proximity of a spiral cooling channel. • Sealing tests have demonstrated adequate helium leak rates up to temperatures of 1000 °C. • Flow modeling indicates a minimal helium pressure drop across the membrane holder (<700 Pa). • Thermal modeling shows good heat removal and minimal membrane temperature variation (±2%) even up to peak TPE ion fluxes. - Abstract: Experiments on retention of hydrogen isotopes (including tritium) at temperatures less than 800 °C have been carried out in the Tritium Plasma Experiment (TPE) at Idaho National Laboratory [1,2]. To provide a direct measurement of plasma driven permeation in plasma facing materials at temperatures reaching 1000 °C, a new TPE membrane holder has been built to hold test specimens (≤1 mm in thickness) at high temperature while measuring tritium permeating through the membrane from the plasma facing side. This measurement is accomplished by employing a carrier gas that transports the permeating tritium from the backside of the membrane to ion chambers giving a direct measurement of the plasma driven tritium permeation rate. Isolation of the membrane cooling and sweep gases from TPE's vacuum chamber has been demonstrated by sealing tests performed up to 1000 °C of a membrane holder design that provides easy change out of membrane specimens between tests. Simulations of the helium carrier gas which transports tritium to the ion chamber indicate a very small pressure drop (∼700 Pa) with good flow uniformity (at 1000 sccm). Thermal transport simulations indicate that temperatures up to 1000 °C are expected at the highest TPE fluxes

  2. Testing of Disposable Protective Garments Against Isocyanate Permeation From Spray Polyurethane Foam Insulation.

    Science.gov (United States)

    Mellette, Michael P; Bello, Dhimiter; Xue, Yalong; Yost, Michael; Bello, Anila; Woskie, Susan

    2018-05-12

    Diisocyanates (isocyanates), including methylene diphenyl diisocyanate (MDI), are the primary reactive components of spray polyurethane foam (SPF) insulation. They are potent immune sensitizers and a leading cause of occupational asthma. Skin exposure to isocyanates may lead to both irritant and allergic contact dermatitis and possibly contribute to systemic sensitization. More than sufficient evidence exists to justify the use of protective garments to minimize skin contact with aerosolized and raw isocyanate containing materials during SPF applications. Studies evaluating the permeation of protective garments following exposure to SPF insulation do not currently exist. To conduct permeation testing under controlled conditions to assess the effectiveness of common protective gloves and coveralls during SPF applications using realistic SPF product formulations. Five common disposable garment materials [disposable latex gloves (0.07 mm thickness), nitrile gloves (0.07 mm), vinyl gloves (0.07 mm), polypropylene coveralls (0.13 mm) and Tyvek coveralls (0.13 mm)] were selected for testing. These materials were cut into small pieces and assembled into a permeation test cell system and coated with a two-part slow-rise spray polyurethane foam insulation. Glass fiber filters (GFF) pretreated with 1-(9-anthracenylmethyl)piperazine) (MAP) were used underneath the garment to collect permeating isocyanates. GFF filters were collected at predetermined test intervals between 0.75 and 20.00 min and subsequently analyzed using liquid chromatography-tandem mass spectrometry. For each garment material, we assessed (i) the cumulative concentration of total isocyanate, including phenyl isocyanate and three MDI isomers, that effectively permeated the material over the test time; (ii) estimated breakthrough detection time, average permeation rate, and standardized breakthrough time; from which (iii) recommendations were developed for the use of similar protective garments following

  3. Water Vapor Permeation of Metal Oxide/Polymer Coated Plastic Films

    Science.gov (United States)

    Numata, Yukihiro; Oya, Toshiyuki; Kuwahara, Mitsuru; Ito, Katsuya

    Barrier performance to water vapor permeation of ceramic coated layers deposited on flexible polymer films is of great interest to food packaging, medical device packaging and flat panel display industries. In this study, a new type film in which a ceramic layer is deposited on a polymer coated film was proposed for lower water vapor permeation. It is important how to control interfacial properties between each layer and film for good barrier performance. Several kinds of polymer coated materials were prepared for changing surface free energy of the films before and after depositing the ceramic layer. The ceramic layer, which is composed of mixed material of SiO2 and Al2O3, was adopted under the same conditions. The following results were obtained; 1) Water vapor permeation is not related to the surface energy of polymer coated films, 2) After depositing the ceramic layer, however, a strong correlation is observed between the water vapor permeation and surface free energy. 3) The phenomenon is considered that the polarity of the polymer layers plays a key role in changing the structure of ceramic coated layers.

  4. Mechanisms of oxygen permeation through plastic films and barrier coatings

    International Nuclear Information System (INIS)

    Wilski, Stefan; Wipperfürth, Jens; Jaritz, Montgomery; Kirchheim, Dennis; Dahlmann, Rainer; Hopmann, Christian; Mitschker, Felix; Awakowicz, Peter

    2017-01-01

    Oxygen and water vapour permeation through plastic films in food packaging or other applications with high demands on permeation are prevented by inorganic barrier films. Most of the permeation occurs through small defects (<3 µ m) in the barrier coating. The defects were visualized by etching with reactive oxygen in a capacitively coupled plasma and subsequent SEM imaging. In this work, defects in SiO x -coatings deposited by plasma-enhanced chemical vapour deposition on polyethylene terephthalate (PET) are investigated and the mass transport through the polymer is simulated in a 3D approach. Calculations of single defects showed that there is no linear correlation between the defect area and the resulting permeability. The influence of adjacent defects in different distances was observed and led to flow reduction functions depending on the defect spacing and defect area. A critical defect spacing where no interaction between defects occurs was found and compared to other findings. According to the superposition principle, the permeability of single defects was added up and compared to experimentally determined oxygen permeation. The results showed the same trend of decreasing permeability with decreasing defect densities. (paper)

  5. In vitro permeation of platinum and rhodium through Caucasian skin.

    Science.gov (United States)

    Franken, A; Eloff, F C; Du Plessis, J; Badenhorst, C J; Jordaan, A; Du Plessis, J L

    2014-12-01

    During platinum group metals (PGMs) refining the possibility exists for dermal exposure to PGM salts. The dermal route has been questioned as an alternative route of exposure that could contribute to employee sensitisation, even though literature has been focused on respiratory exposure. This study aimed to investigate the in vitro permeation of platinum and rhodium through intact Caucasian skin. A donor solution of 0.3mg/ml of metal, K2PtCl4 and RhCl3 respectively, was applied to the vertical Franz diffusion cells with full thickness abdominal skin. The receptor solution was removed at various intervals during the 24h experiment, and analysed with high resolution ICP-MS. Skin was digested and analysed by ICP-OES. Results indicated cumulative permeation with prolonged exposure, with a significantly higher mass of platinum permeating after 24h when compared to rhodium. The mass of platinum retained inside the skin and the flux of platinum across the skin was significantly higher than that of rhodium. Permeated and skin retained platinum and rhodium may therefore contribute to sensitisation and indicates a health risk associated with dermal exposure in the workplace. Copyright © 2014 Elsevier Ltd. All rights reserved.

  6. Changes in the physical properties of the dynamic layer and its correlation with permeate quality in a self-forming dynamic membrane bioreactor.

    Science.gov (United States)

    Guan, Dao; Dai, Ji; Watanabe, Yoshimasa; Chen, Guanghao

    2018-09-01

    The self-forming dynamic membrane bioreactor (SFDMBR) is a biological wastewater treatment technology based on the conventional membrane bioreactor (MBR) with membrane material modification to a large pore size (30-100 μm). This modification requires a dynamic layer formed by activated sludge to provide effective filtration function for high-quality permeate production. The properties of the dynamic layer are therefore important for permeate quality in SFDMBRs. The interaction between the structure of the dynamic layer and the performance of SFDMBRs is little known but understandably complex. To elucidate the interaction, a lab-scale SFDMBR system coupled with a nylon woven mesh as the supporting material was operated. After development of a mature dynamic layer, excellent solid-liquid separation was achieved, as evidenced by a low permeate turbidity of less than 2 NTU. The permeate turbidity stayed below this level for nearly 80 days. In the fouling phase, the dynamic layer was compressed with an increase in the trans-membrane pressure and the quality of the permeate kept deteriorating until the turbidity exceeded 10 NTU. The investigation revealed that the majority of permeate particles were dissociated from the dynamic layer on the back surface of the supporting material, which is caused by the compression, breakdown, and dissociation of the dynamic layer. This phenomenon was observed directly in experiment instead of model prediction or conjecture for the first time. Copyright © 2018 Elsevier Ltd. All rights reserved.

  7. Assessment of permeation of lipoproteins in human carotid tissue

    Science.gov (United States)

    Ghosn, Mohamad G.; Syed, Saba H.; Leba, Michael; Morrisett, Joel D.; Tuchin, Valery V.; Larin, Kirill V.

    2010-02-01

    Cardiovascular disease is among the leading causes of death in the United States. Specifically, atherosclerosis is an increasingly devastating contributor to the tally and has been found to be a byproduct of arterial permeability irregularities in regards to lipoprotein penetration. To further explore arterial physiology and molecular transport, the imaging technique of Optical Coherence Tomography (OCT) was employed. With OCT, the permeation of glucose (MW = 180 Da), low density lipoprotein (LDL; MW = 2.1 × 106 Da), and high density lipoprotein (HDL; MW = 2.5 × 105 Da) in human carotid tissue was studied to determine the effect of different molecular characteristics on permeation in atherosclerotic tissues. The permeability rates calculated from the diffusion of the molecular agents into the abnormal carotid tissue samples is compared to those of normal, healthy tissue. The results show that in the abnormal tissue, the permeation of agents correlate to the size constraints. The larger molecules of LDL diffuse the slowest, while the smallest molecules of glucose diffuse the fastest. However, in normal tissue, LDL permeates at a faster rate than the other two agents, implying the existence of a transport mechanism that facilitates the passage of LDL molecules. These results highlight the capability of OCT as a sensitive and specific imaging technique as well as provide significant information to the understanding of atherosclerosis and its effect on tissue properties.

  8. Effect of argon ion sputtering of surface on hydrogen permeation through vanadium

    International Nuclear Information System (INIS)

    Yamawaki, Michio; Namba, Takashi; Yoneoka, Toshiaki; Kanno, Masayoshi; Shida, Koji.

    1983-01-01

    In order to measure the hydrogen permeation rate through V with atomically cleaned surface, an Ar ion sputtering apparatus has been installed in the hydrogen permeability measuring system. The permeation rate of the initial specimen was found to be increased by about one order of magnitude after Ar ion sputtering of its upstream side surface. Repeating of such a sputter-cleaning was not so much effective in increasing the steady state permeation rate as the initial sputtering was, but it accelerated the transient response rate by a factor of 2 or 3. The transient response rate was also accelerated by the increase of hydrogen pressure, but this effect tended to be diminished by the sputter-cleaning of specimen surface. The surface impurity layer on the downstream side of specimen was also inferred to act as a diffusion barrier affecting the steady state permeation rate. The present value of activation energy for hydrogen permeation through V at temperatures below 873K was the smallest one ever obtained, showing that the surface effect was minimized in the present study on account of the surface sputter-cleaning in addition to the ultra high vacuum system. (author)

  9. DMPy: a Python package for automated mathematical model construction of large-scale metabolic systems.

    Science.gov (United States)

    Smith, Robert W; van Rosmalen, Rik P; Martins Dos Santos, Vitor A P; Fleck, Christian

    2018-06-19

    Models of metabolism are often used in biotechnology and pharmaceutical research to identify drug targets or increase the direct production of valuable compounds. Due to the complexity of large metabolic systems, a number of conclusions have been drawn using mathematical methods with simplifying assumptions. For example, constraint-based models describe changes of internal concentrations that occur much quicker than alterations in cell physiology. Thus, metabolite concentrations and reaction fluxes are fixed to constant values. This greatly reduces the mathematical complexity, while providing a reasonably good description of the system in steady state. However, without a large number of constraints, many different flux sets can describe the optimal model and we obtain no information on how metabolite levels dynamically change. Thus, to accurately determine what is taking place within the cell, finer quality data and more detailed models need to be constructed. In this paper we present a computational framework, DMPy, that uses a network scheme as input to automatically search for kinetic rates and produce a mathematical model that describes temporal changes of metabolite fluxes. The parameter search utilises several online databases to find measured reaction parameters. From this, we take advantage of previous modelling efforts, such as Parameter Balancing, to produce an initial mathematical model of a metabolic pathway. We analyse the effect of parameter uncertainty on model dynamics and test how recent flux-based model reduction techniques alter system properties. To our knowledge this is the first time such analysis has been performed on large models of metabolism. Our results highlight that good estimates of at least 80% of the reaction rates are required to accurately model metabolic systems. Furthermore, reducing the size of the model by grouping reactions together based on fluxes alters the resulting system dynamics. The presented pipeline automates the

  10. Control of tritium permeation through fusion reactor strucural materials

    International Nuclear Information System (INIS)

    Maroni, V.A.

    1978-01-01

    The intention of this paper is to provide a brief synopsis of the status of understanding and technology pertaining to the dissolution and permeation of tritium in fusion reactor materials. The following sections of this paper attempt to develop a simple perspective for understanding the consequences of these phenomena and the nature of the technical methodology being contemplated to control their impact on fusion reactor operation. Considered in order are: (1) the occurrence of tritium in the fusion fuel cycle, (2) a set of tentative criteria to guide the analysis of tritium containment and control strategies, (3) the basic mechanisms by which tritium may be released from a fusion plant, and (4) the methods currently under development to control the permeation-related release mechanisms. To provide background and support for these considerations, existing solubility and permeation data for the hydrogen isotopes are compared and correlated under conditions to be expected in fusion reactor systems

  11. RF plasma-driven hydrogen permeation through a biased iron membrane

    International Nuclear Information System (INIS)

    Banno, T.; Waelbroeck, F.; Winter, J.

    1984-01-01

    The steady-state RF plasma-driven hydrogen permeation through an electrically biased iron membrane has been investigated as a function of the bias potential Vsub(M) for membrane temperatures in the range of 150-400 0 C. Vsub(M) has been gradually increased positively from the floating potential of the membrane. The permeation flux decreases when Vsub(M) increases at low voltages: positive hydrogen ions are repelled. The membrane temperature does not influence this effect measurably. The permeation flux starts to increase when Vsub(M) is raised higher, i.e. when energetic electrons strike the surface. This phenomenon shows a pronounced temperature dependence - the enhancement is largest for the lowest temperatures. The effect is interpreted in terms of an electron-induced dissociation of hydrogen molecules on the membrane surface. (orig.)

  12. Permeation of macromolecules into the renal glomerular basement membrane and capture by the tubules

    Science.gov (United States)

    Lawrence, Marlon G.; Altenburg, Michael K.; Sanford, Ryan; Willett, Julian D.; Bleasdale, Benjamin; Ballou, Byron; Wilder, Jennifer; Li, Feng; Miner, Jeffrey H.; Berg, Ulla B.; Smithies, Oliver

    2017-01-01

    How the kidney prevents urinary excretion of plasma proteins continues to be debated. Here, using unfixed whole-mount mouse kidneys, we show that fluorescent-tagged proteins and neutral dextrans permeate into the glomerular basement membrane (GBM), in general agreement with Ogston's 1958 equation describing how permeation into gels is related to molecular size. Electron-microscopic analyses of kidneys fixed seconds to hours after injecting gold-tagged albumin, negatively charged gold nanoparticles, and stable oligoclusters of gold nanoparticles show that permeation into the lamina densa of the GBM is size-sensitive. Nanoparticles comparable in size with IgG dimers do not permeate into it. IgG monomer-sized particles permeate to some extent. Albumin-sized particles permeate extensively into the lamina densa. Particles traversing the lamina densa tend to accumulate upstream of the podocyte glycocalyx that spans the slit, but none are observed upstream of the slit diaphragm. At low concentrations, ovalbumin-sized nanoparticles reach the primary filtrate, are captured by proximal tubule cells, and are endocytosed. At higher concentrations, tubular capture is saturated, and they reach the urine. In mouse models of Pierson’s or Alport’s proteinuric syndromes resulting from defects in GBM structural proteins (laminin β2 or collagen α3 IV), the GBM is irregularly swollen, the lamina densa is absent, and permeation is increased. Our observations indicate that size-dependent permeation into the lamina densa of the GBM and the podocyte glycocalyx, together with saturable tubular capture, determines which macromolecules reach the urine without the need to invoke direct size selection by the slit diaphragm. PMID:28246329

  13. Tritium permeation evaluation through vertical target of divertor based on recent tritium transport properties

    International Nuclear Information System (INIS)

    Nakamura, Hirofumi; Nishi, Masataka

    2003-11-01

    Re-evaluation of tritium permeation through vertical target of divertor under the ITER operation condition was carried out using tritium properties in the candidate materials such as the diffusion coefficient and the trapping factors in tungsten for armor, and the surface recombination coefficient on copper for the heat sink obtained by authours' recent investigation (authors' data), which simulated the plasma-facing conditions of ITER. Evaluation with the data set of previous evaluation was also carried out for comparison (previous data). The permeation analysis was carried out individually by classifying into the armor region (Carbon Fiber Composites and tungsten) and the slit region without armor (3% of armor surface area) assuming the incident flux and temperature for each region. As the results of the permeation analysis, estimated permeation amount with the authors' data was one order less than that with the previous data at the end of lifetime of the divertor due to authors' small diffusion coefficient of tritium in tungsten. It also indicated the possibility that permeation through the slit region of the armor tiles could dominate total permeation through the vertical target, since tritium permeation amount through tungsten armor with the authors' data was estimated to be reduced drastically smaller than that with the previous evaluation data. The result of a little tritium permeation amount through the vertical target with the authors' data ensured the conservatism of the current evaluation of tritium concentration in the primary cooling water in ITER divertor, as it indicated the possibility of direct drainage of the divertor primary cooling water. (author)

  14. In Vitro Skin Permeation Enhancement of Sumatriptan by Microneedle Application.

    Science.gov (United States)

    Nalluri, Buchi N; Anusha, Sai Sri V; Bramhini, Sri R; Amulya, J; Sultana, Ashraf S K; Teja, Chandra U; Das, Diganta B

    2015-01-01

    Different dimensions of commercially available microneedle devices, namely, Admin- Patch(®) microneedle arrays (MN) (0.6, 0.9, 1.2 and 1.5 mm lengths) and Dermaroller(®) microneedle rollers (DR) (0.5 and 1mm lengths) were evaluated for their relative efficiency in enhancement of transdermal permeation of Sumatriptan (SMT). Solubility assessment of SMT was carried out using propylene glycol (PG), polyethylene glycol (PEG) in combination with saline (S) at different ratios and the order of solubility was found to be 70:30 > 80:20 > 90:10 %v/v in both PG:S and PEG:S. In vitro skin permeation studies were performed using PG:S (70:30 %v/v) as donor vehicle. A significant increase in cumulative amount of SMT permeated, steady state flux, permeability coefficient and diffusion coefficient values were observed after microneedle treatment, and the values were in the order of 1.5mm MN >1.2mm MN >0.9mm MN >1mm DR >0.6mm MN >0.5mm DR > passive permeation. Lag times were significantly shorter after longer microneedle application (0.24h for 1.5mm MN). Arrays were found to be superior to rollers with similar microneedle lengths in enhancing SMT permeation and may be attributed to higher density of microneedles and force of application onto skin. The in vitro flux values revealed that 2.5cm(2) area patch is sufficient for effective therapy after treatment of skin with 1.5mm MN. It may be inferred that microneedle application significantly enhances the transdermal penetration of SMT and that it may be feasible to deliver clinically relevant therapeutic levels of SMT using microneedle assisted transdermal delivery systems.

  15. Seawater ultrafiltration fouling control: Backwashing with demineralized water/SWRO permeate

    KAUST Repository

    Li, Sheng; Heijman, Sebastiaan G J; Verberk, J. Q J C; Amy, Gary L.; Van Dijk, Johannis C.

    2012-01-01

    In this study, the effect of demineralized water backwashing on fouling control of seawater ultrafiltration was investigated. Seawater from Scheveningen beach in The Hague and a desalination plant of Evides Company at Zeeland in the Netherlands was used as feed water, while demineralized water and UF permeate were used as backwash water for a fouling control efficiency comparison under different fluxes and backwash durations. Furthermore, demineralized waters with 5 or 50 mmol/l NaCl were applied for backwashing as well, to check the influence of monovalent cations on UF fouling control. Additionally, SWRO permeate was used for backwashes in long-term experiments to check the possibility of it replacing demineralized water. Results show that seawater UF fouling control is substantially improved by demineralized water backwashing. However, due to the high salinity of seawater, more water was required to dilute the cation concentration and limit the dispersion effect near the membrane surface than was needed for surface water. A 2-min demineralized water backwash showed better fouling control efficiency than a 1-min backwash. Furthermore, the presence of monovalent cations in the backwash water deteriorated the fouling control efficiency of the backwash, indicating the existence of a charge screening effect. The demineralized water with 5 and 50 mmol/l NaCl both showed a similar fouling control efficiency which is better than the UF permeate backwash. The calcium ions in UF permeate probably deteriorates the fouling control efficiency by maintaining a Ca-bridging effect between the membranes and NOM. SWRO permeate backwashing successfully controls membrane fouling as well. © 2012 Elsevier B.V. All rights reserved.

  16. Seawater ultrafiltration fouling control: Backwashing with demineralized water/SWRO permeate

    KAUST Repository

    Li, Sheng

    2012-09-01

    In this study, the effect of demineralized water backwashing on fouling control of seawater ultrafiltration was investigated. Seawater from Scheveningen beach in The Hague and a desalination plant of Evides Company at Zeeland in the Netherlands was used as feed water, while demineralized water and UF permeate were used as backwash water for a fouling control efficiency comparison under different fluxes and backwash durations. Furthermore, demineralized waters with 5 or 50 mmol/l NaCl were applied for backwashing as well, to check the influence of monovalent cations on UF fouling control. Additionally, SWRO permeate was used for backwashes in long-term experiments to check the possibility of it replacing demineralized water. Results show that seawater UF fouling control is substantially improved by demineralized water backwashing. However, due to the high salinity of seawater, more water was required to dilute the cation concentration and limit the dispersion effect near the membrane surface than was needed for surface water. A 2-min demineralized water backwash showed better fouling control efficiency than a 1-min backwash. Furthermore, the presence of monovalent cations in the backwash water deteriorated the fouling control efficiency of the backwash, indicating the existence of a charge screening effect. The demineralized water with 5 and 50 mmol/l NaCl both showed a similar fouling control efficiency which is better than the UF permeate backwash. The calcium ions in UF permeate probably deteriorates the fouling control efficiency by maintaining a Ca-bridging effect between the membranes and NOM. SWRO permeate backwashing successfully controls membrane fouling as well. © 2012 Elsevier B.V. All rights reserved.

  17. Permeation of iodide from iodine-enriched yeast through porcine intestine.

    Science.gov (United States)

    Ryszka, Florian; Dolińska, Barbara; Zieliński, Michał; Chyra, Dagmara; Dobrzański, Zbigniew

    2013-01-01

    Iodine deficiency is a common phenomenon, threatening the whole global human population. Recommended daily intake of iodine is 150 μg for adults and 250 μg for pregnant and breastfeeding women. About 50% of human population can be at risk of moderate iodine deficiency. Due to this fact, increased iodine supplementation is recommended, through intake of iodized mineral water and salt iodization. The aim of this study was to investigate permeation and absorption of iodide from iodine bioplex (experimental group) in comparison with potassium iodide (controls). Permeation and absorption processes were investigated in vitro using a porcine intestine. The experimental model was based on a standard Franz diffusion cell (FD-Cell). The iodine bioplex was produced using Saccharomyces cerevisiae yeast and whey powder: iodine content - 388 μg/g, total protein - 28.5%, total fat - 0.9%., glutamic acid - 41.2%, asparaginic acid - 29.4%, lysine - 24.8%; purchased from: F.Z.N.P. Biochefa, Sosnowiec, Poland. Potassium iodide was used as controls, at 388 μg iodine concentration, which was the same as in iodine-enriched yeast bioplex. A statistically significant increase in iodide permeation was observed for iodine-enriched yeast bioplex in comparison with controls - potassium iodide. After 5h the total amount of permeated iodide from iodine-enriched yeast bioplex was 85%, which is ~ 2-fold higher than controls - 37%. Iodide absorption was by contrast statistically significantly higher in controls - 7.3%, in comparison with 4.5% in experimental group with iodine-enriched yeast bioplex. Presented results show that iodide permeation process dominates over absorption in case of iodine-enriched yeast bioplex.

  18. In vitro permeation of platinum through African and Caucasian skin.

    Science.gov (United States)

    Franken, A; Eloff, F C; du Plessis, J; Badenhorst, C J; Du Plessis, J L

    2015-02-03

    The majority of the South African workforce are Africans, therefore potential racial differences should be considered in risk and exposure assessments in the workplace. Literature suggests African skin to be a superior barrier against permeation and irritants. Previous in vitro studies on metals only included skin from Caucasian donors, whereas this study compared the permeation of platinum through African and Caucasian skin. A donor solution of 0.3 mg/ml of potassium tetrachloroplatinate (K₂PtCl₄) dissolved in synthetic sweat was applied to the vertical Franz diffusion cells with full thickness abdominal skin. Skin from three female African and three female Caucasian donors were included (n=21). The receptor solution was removed at various intervals during the 24 h experiment, and analysed with high resolution inductively coupled plasma-mass spectrometry (ICP-MS). Skin was digested and analysed by inductively coupled plasma-optical emission spectrometry (ICP-OES). Significantly higher permeation of platinum through intact African skin (p=0.044), as well as a significantly higher mass of platinum retention in African skin in comparison with Caucasian skin (p=0.002) occurred. Significant inter-donor variation was found in both racial groups (pskin and further investigation is necessary to explain the higher permeation through African skin. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  19. Hydrogen permeation through sol-gel-coated iron during galvanostatic charging

    International Nuclear Information System (INIS)

    Zakorchemna, I.; Carmona, N.; Zakroczymski, T.

    2008-01-01

    One-layer sol-gel silica-zirconia and two-layer silica-zirconia and zirconia coatings were deposited on one side of iron membranes by spin-coating, densified in air and annealed up to 800 deg. C in vacuum. Hydrogen permeation through the membranes, coated and uncoated, polarised cathodically under galvanostatic control in 0.1 M NaOH solution was studied using the electrochemical permeation technique. During the initial period, the effect of the sol-gel coatings was insignificant. However, the coatings quite efficiently prevented the iron surface become more active to hydrogen entry during a long-lasting cathodic polarisation. In addition, the electrochemical-corrosion behaviour of the coated iron and the effect of the sol-gel coatings on the effective diffusivity of hydrogen in the coated membranes were studied. On the basis of the polarisation curves and the hydrogen permeation data it was proved that the sol-gel coatings blocked the iron surface for the hydrogen evolution reaction and, consequently, for the hydrogen entry into iron. The effective coating coverage was determined by comparison of the hydrogen fluxes permeating the coated and uncoated membranes. Finally the real concentration of hydrogen beneath the uncoated iron sites and the amount of hydrogen stored in a membrane were evaluated

  20. Dissolution and permeation characteristics of artemether tablets ...

    African Journals Online (AJOL)

    characterized by delayed drug release but enhanced permeation of the released drug. Keywords: ... and prosopis gum as binders and to consider the relationship between ..... higher tensile strength and higher brittle fracture index compared ...

  1. Crystallization and deuterium permeation behaviors of yttrium oxide coating prepared by metal organic decomposition

    Directory of Open Access Journals (Sweden)

    Takumi Chikada

    2016-12-01

    Full Text Available Yttrium oxide coatings were fabricated on reduced activation ferritic/martensitic steels by metal organic decomposition with a dip-coating technique, and their deuterium permeation behaviors were investigated. The microstructure of the coatings varied with heat-treatment temperature: amorphous at 670ºC (amorphous coating and crystallized at 700ºC (crystallized coating. Deuterium permeation flux of the amorphous coating was lower than the uncoated steel by a factor of 5 at 500ºC, while that of the crystallized coating was lower by a factor of around 100 at 400‒550ºC. The permeation fluxes of both coatings were drastically decreased during the measurements at higher temperatures by a factor of up to 790 for the amorphous coating and 1000 for the crystallized one, indicating a microstructure modification occurred by an effect of test temperature with hydrogen flux. Temperature dependence of deuterium diffusivity in the coatings suggests that the decrease of the permeation flux has been derived from a decrease of the diffusivity. Characteristic permeation behaviors were observed with different annealing conditions; however, they can be interpreted using the permeation mechanism clarified in the previous erbium oxide coating studies.

  2. Improved permeation barriers for tritiated waste packaging

    International Nuclear Information System (INIS)

    Vassallo, G.; Van Den Bergh, R.; Forcey, K.S.; Perujo, A.

    1994-01-01

    High-density polyethylene (HDPE) is extensively used as flexible bagging or packaging for soft tritiated waste in the tritium community because of its low permeability to the more radiotoxic form of tritium, i.e., tritiated water (HTO). However, HDPE does not represent a perfect barrier to HTO nor does it effectively hinder the permeation of elemental tritium, i.e, HT. This latter drawback is particularly important considering that the elemental form may readily convert to HTO outside of the waste package. The possible use of a multilayer film as packing material for the conditioning of tritiated waste is assessed, and its capability to hinder the permeation of elemental tritium is measured and compared with that of bare HDPE. The material investigated is readily available from the food industry. 5 refs., 1 tab

  3. Differential permeation of piroxicam-loaded PLGA micro/nanoparticles and their in vitro enhancement

    International Nuclear Information System (INIS)

    Shankarayan, Raju; Kumar, Sumit; Mishra, Prashant

    2013-01-01

    Piroxicam is a non-steroidal anti-inflammatory drug used for the treatment of musculoskeletal pain. The main problem encountered when piroxicam is administered orally is its gastric side-effect (ulcer, bleeding and holes in the stomach). Transmucosal delivery and encapsulation of piroxicam in biodegradable particles offer potential advantages over conventional oral delivery. The present study was aimed to develop an alternative to piroxicam-delivery which could overcome the direct contact of the drug at the mucosal membrane and its permeation through the mucosal membrane was studied. To achieve this, the piroxicam was encapsulated in Poly (lactide-co-glycolide) (PLGA) microparticles (size 1–4 μm, encapsulation efficiency 80–85 %) and nanoparticles (size 151.6 ± 28.6 nm, encapsulation efficiency 92.17 ± 3.08 %). Various formulation process parameters were optimised for the preparation of piroxicam-loaded PLGA nanoparticles of optimal size and encapsulation efficiency. Transmucosal permeability of piroxicam-loaded PLGA micro- and nanoparticles through the porcine oesophageal mucosa was studied. Using fluorescently labelled PLGA micro- and nanoparticles, size-dependent permeation was demonstrated. Furthermore, the effect of different permeation enhancers on the flux rate and permeability coefficient for the permeation of nanoparticles was investigated. The results suggested that amongst the permeation enhancers used the most efficient enhancement of permeation was observed with 10 mM sodium dodecyl sulphate.

  4. Differential permeation of piroxicam-loaded PLGA micro/nanoparticles and their in vitro enhancement

    Energy Technology Data Exchange (ETDEWEB)

    Shankarayan, Raju; Kumar, Sumit; Mishra, Prashant, E-mail: pmishra@dbeb.iitd.ac.in [Indian Institute of Technology Delhi, Department of Biochemical Engineering and Biotechnology (India)

    2013-03-15

    Piroxicam is a non-steroidal anti-inflammatory drug used for the treatment of musculoskeletal pain. The main problem encountered when piroxicam is administered orally is its gastric side-effect (ulcer, bleeding and holes in the stomach). Transmucosal delivery and encapsulation of piroxicam in biodegradable particles offer potential advantages over conventional oral delivery. The present study was aimed to develop an alternative to piroxicam-delivery which could overcome the direct contact of the drug at the mucosal membrane and its permeation through the mucosal membrane was studied. To achieve this, the piroxicam was encapsulated in Poly (lactide-co-glycolide) (PLGA) microparticles (size 1-4 {mu}m, encapsulation efficiency 80-85 %) and nanoparticles (size 151.6 {+-} 28.6 nm, encapsulation efficiency 92.17 {+-} 3.08 %). Various formulation process parameters were optimised for the preparation of piroxicam-loaded PLGA nanoparticles of optimal size and encapsulation efficiency. Transmucosal permeability of piroxicam-loaded PLGA micro- and nanoparticles through the porcine oesophageal mucosa was studied. Using fluorescently labelled PLGA micro- and nanoparticles, size-dependent permeation was demonstrated. Furthermore, the effect of different permeation enhancers on the flux rate and permeability coefficient for the permeation of nanoparticles was investigated. The results suggested that amongst the permeation enhancers used the most efficient enhancement of permeation was observed with 10 mM sodium dodecyl sulphate.

  5. Differential permeation of piroxicam-loaded PLGA micro/nanoparticles and their in vitro enhancement

    Science.gov (United States)

    Shankarayan, Raju; Kumar, Sumit; Mishra, Prashant

    2013-03-01

    Piroxicam is a non-steroidal anti-inflammatory drug used for the treatment of musculoskeletal pain. The main problem encountered when piroxicam is administered orally is its gastric side-effect (ulcer, bleeding and holes in the stomach). Transmucosal delivery and encapsulation of piroxicam in biodegradable particles offer potential advantages over conventional oral delivery. The present study was aimed to develop an alternative to piroxicam-delivery which could overcome the direct contact of the drug at the mucosal membrane and its permeation through the mucosal membrane was studied. To achieve this, the piroxicam was encapsulated in Poly (lactide- co-glycolide) (PLGA) microparticles (size 1-4 μm, encapsulation efficiency 80-85 %) and nanoparticles (size 151.6 ± 28.6 nm, encapsulation efficiency 92.17 ± 3.08 %). Various formulation process parameters were optimised for the preparation of piroxicam-loaded PLGA nanoparticles of optimal size and encapsulation efficiency. Transmucosal permeability of piroxicam-loaded PLGA micro- and nanoparticles through the porcine oesophageal mucosa was studied. Using fluorescently labelled PLGA micro- and nanoparticles, size-dependent permeation was demonstrated. Furthermore, the effect of different permeation enhancers on the flux rate and permeability coefficient for the permeation of nanoparticles was investigated. The results suggested that amongst the permeation enhancers used the most efficient enhancement of permeation was observed with 10 mM sodium dodecyl sulphate.

  6. Chromatic response of polydiacetylene vesicle induced by the permeation of methotrexate.

    Science.gov (United States)

    Shin, Min Jae; Kim, Ye Jin; Kim, Jong-Duk

    2015-07-07

    The noble vesicular system of polydiacetylene showed a red shift using two types of detecting systems. One of the systems involves the absorption of target materials from the outer side of the vesicle, and the other system involves the permeation through the vesicular layers from within the vesicle. The chromatic mixed vesicles of N-(2-aminoethyl)pentacosa-10,12-diynamide (AEPCDA) and dimethyldioctadecylammonium chloride (DODAC) were fabricated by sonication, followed by polymerization by UV irradiation. The stability of monomeric vesicles was observed to increase with the polymerization of the vesicles. Methotrexate was used as a target material. The polymerized mixed vesicles having a blue color were exposed to a concentration gradient of methotrexate, and a red shift was observed indicating the adsorption of methotrexate on the polydiacetylene bilayer. In order to check the chromatic change by the permeation of methotrexate, we separated the vesicle portion, which contained methotrexate inside the vesicle, and checked chromatic change during the permeation of methotrexate through the vesicle. The red shift apparently indicates the disturbance in the bilayer induced by the permeation of methotrexate. The maximum contrast of color appeared at the equal molar ratio of AEPCDA and DODAC, indicating that the formation of flexible and deformable vesicular layers is important for red shift. Therefore, it is hypothesized that the system can be applicable for the chromatic detection of the permeation of methotrexate through the polydiacetylene layer.

  7. Financial Permeation and Economic Growth: Evidence from Sub-Saharan Africa

    OpenAIRE

    Inoue, Takeshi; Hamori, Shigeyuki

    2013-01-01

    This article empirically analyzes the role of finance in economic growth in Sub-Saharan Africa from the perspective of what is termed herein “financial permeation”. By estimating panel data on 37 countries in Sub-Saharan Africa between 2004 and 2010, we examine whether financial permeation through improved convenience and access to financial services has contributed to economic growth in this region. Empirical results clearly indicate that financial permeation has a statistically significant ...

  8. Measurement of tritium permeation through resistant materials near room temperature

    International Nuclear Information System (INIS)

    Maienschein, J.; DuVal, V.; McMurphy, F.; Uribe, F.; Musket, R.; Brown, D.

    1985-01-01

    To measure tritium permeation through low-permeability materials at 50 to 170 0 C, we use highly-sensitive liquid scintillation counting to detect the permeating tritium. To validate our method, we conducted extensive experiments with copper, for which much data exists for comparison. We report permeability of tritium through copper at 50, 100, and 170 0 C, and discuss details of the experimental technique. Further plans are outlined. 15 refs., 5 figs., 1 tab

  9. Rationalizing the permeation of polar antibiotics into Gram-negative bacteria

    International Nuclear Information System (INIS)

    Scorciapino, Mariano Andrea; Acosta-Gutierrez, Silvia; Benkerrou, Dehbia; D’Agostino, Tommaso; Malloci, Giuliano; Samanta, Susruta; Bodrenko, Igor; Ceccarelli, Matteo

    2017-01-01

    The increasing level of antibiotic resistance in Gram-negative bacteria, together with the lack of new potential drug scaffolds in the pipeline, make the problem of infectious diseases a global challenge for modern medicine. The main reason that Gram-negative bacteria are particularly challenging is the presence of an outer cell-protecting membrane, which is not present in Gram-positive species. Such an asymmetric bilayer is a highly effective barrier for polar molecules. Several protein systems are expressed in the outer membrane to control the internal concentration of both nutrients and noxious species, in particular: (i) water-filled channels that modulate the permeation of polar molecules and ions according to concentration gradients, and (ii) efflux pumps to actively expel toxic compounds. Thus, besides expressing specific enzymes for drugs degradation, Gram-negative bacteria can also resist by modulating the influx and efflux of antibiotics, keeping the internal concentration low. However, there are no direct and robust experimental methods capable of measuring the permeability of small molecules, thus severely limiting our knowledge of the molecular mechanisms that ultimately control the permeation of antibiotics through the outer membrane. This is the innovation gap to be filled for Gram-negative bacteria. This review is focused on the permeation of small molecules through porins, considered the main path for the entry of polar antibiotics into Gram-negative bacteria. A fundamental understanding of how these proteins are able to filter small molecules is a prerequisite to design/optimize antibacterials with improved permeation. The level of sophistication of modern molecular modeling algorithms and the advances in new computer hardware has made the simulation of such complex processes possible at the molecular level. In this work we aim to share our experience and perspectives in the context of a multidisciplinary extended collaboration within the IMI

  10. Rationalizing the permeation of polar antibiotics into Gram-negative bacteria

    Science.gov (United States)

    Scorciapino, Mariano Andrea; Acosta-Gutierrez, Silvia; Benkerrou, Dehbia; D'Agostino, Tommaso; Malloci, Giuliano; Samanta, Susruta; Bodrenko, Igor; Ceccarelli, Matteo

    2017-03-01

    The increasing level of antibiotic resistance in Gram-negative bacteria, together with the lack of new potential drug scaffolds in the pipeline, make the problem of infectious diseases a global challenge for modern medicine. The main reason that Gram-negative bacteria are particularly challenging is the presence of an outer cell-protecting membrane, which is not present in Gram-positive species. Such an asymmetric bilayer is a highly effective barrier for polar molecules. Several protein systems are expressed in the outer membrane to control the internal concentration of both nutrients and noxious species, in particular: (i) water-filled channels that modulate the permeation of polar molecules and ions according to concentration gradients, and (ii) efflux pumps to actively expel toxic compounds. Thus, besides expressing specific enzymes for drugs degradation, Gram-negative bacteria can also resist by modulating the influx and efflux of antibiotics, keeping the internal concentration low. However, there are no direct and robust experimental methods capable of measuring the permeability of small molecules, thus severely limiting our knowledge of the molecular mechanisms that ultimately control the permeation of antibiotics through the outer membrane. This is the innovation gap to be filled for Gram-negative bacteria. This review is focused on the permeation of small molecules through porins, considered the main path for the entry of polar antibiotics into Gram-negative bacteria. A fundamental understanding of how these proteins are able to filter small molecules is a prerequisite to design/optimize antibacterials with improved permeation. The level of sophistication of modern molecular modeling algorithms and the advances in new computer hardware has made the simulation of such complex processes possible at the molecular level. In this work we aim to share our experience and perspectives in the context of a multidisciplinary extended collaboration within the IMI

  11. Hydrogen permeation through Flinabe fluoride molten salts for blanket candidates

    Energy Technology Data Exchange (ETDEWEB)

    Nishiumi, Ryosuke, E-mail: r.nishiumi@aees.kyushu-u.ac.jp; Fukada, Satoshi; Nakamura, Akira; Katayama, Kazunari

    2016-11-01

    Highlights: • H{sub 2} diffusivity, solubility and permeability in Flinabe as T breeder are determined. • Effects in composition differences among Flibe, Fnabe and Flinabe are compared. • Changes of pressure dependence of Flinabe permeation rate are clarified. - Abstract: Fluoride molten salt Flibe (2LiF + BeF{sub 2}) is a promising candidate for the liquid blanket of a nuclear fusion reactor, because of its large advantages of tritium breeding ratio and heat-transfer fluid. Since its melting point is higher than other liquid candidates, another new fluoride molten salt Flinabe (LiF + NaF + BeF{sub 2}) is recently focused on because of its lower melting point while holding proper breeding properties. In this experiment, hydrogen permeation behavior through the three molten salts of Flibe (2LiF + BeF{sub 2}), Fnabe (NaF + BeF{sub 2}) and Flinabe are investigated in order to clarify the effects of their compositions on hydrogen transfer properties. After making up any of the three molten salts and purifying it using HF, hydrogen permeability, diffusivity and solubility of the molten salts are determined experimentally by using a system composed of tertiary cylindrical tubes. Close agreement is obtained between experimental data and analytical solutions. H{sub 2} permeability, diffusivity and solubility are correlated as a function of temperature and are compared among the three molten salts.

  12. Changes in chemical permeation of disposable latex, nitrile, and vinyl gloves exposed to simulated movement.

    Science.gov (United States)

    Phalen, Robert N; Le, Thi; Wong, Weng Kee

    2014-01-01

    Glove movement can affect chemical permeation of organic compounds through polymer glove products. However, conflicting reports make it difficult to compare the effects of movement on chemical permeation through commonly available glove types. The aim of this study was to evaluate the effect of movement on chemical permeation of an organic solvent through disposable latex, nitrile, and vinyl gloves. Simulated whole-glove permeation testing was conducted using ethyl alcohol and a previously designed permeation test system. With exposure to movement, a significant decrease (p ≤ 0.001) in breakthrough time (BT) was observed for the latex (-23%) and nitrile gloves (-31%). With exposure to movement, only the nitrile glove exhibited a significant increase (p ≤ 0.001) in steady-state permeation rate (+47%) and cumulative permeation at 30 min (+111%). Even though the nitrile glove provided optimum chemical resistance against ethyl alcohol, it was most affected by movement. With exposure to movement, the latex glove was an equivalent option for overall worker protection, because it was less affected by movement and the permeation rate was lower than that of the nitrile glove. In contrast, the vinyl glove was the least affected by movement, but did not provide adequate chemical resistance to ethyl alcohol in comparison with the nitrile and latex gloves. Glove selection should take movement and polymer type into account. Some glove polymer types are less affected by movement, most notably the latex glove in this test. With nitrile gloves, at least a factor of three should be used when attempting to assign a protection factor when repetitive hand motions are anticipated. Ultimately, the latex gloves outperformed nitrile and vinyl in these tests, which evaluated the effect of movement on chemical permeation. Future research should aim to resolve some of the observed discrepancies in test results with latex and vinyl gloves.

  13. Modeling the Effects of Interfacial Characteristics on Gas Permeation Behavior of Nanotube-Mixed Matrix Membranes.

    Science.gov (United States)

    Chehrazi, Ehsan; Sharif, Alireza; Omidkhah, Mohammadreza; Karimi, Mohammad

    2017-10-25

    Theoretical approaches that accurately predict the gas permeation behavior of nanotube-containing mixed matrix membranes (nanotube-MMMs) are scarce. This is mainly due to ignoring the effects of nanotube/matrix interfacial characteristics in the existing theories. In this paper, based on the analogy of thermal conduction in polymer composites containing nanotubes, we develop a model to describe gas permeation through nanotube-MMMs. Two new parameters, "interfacial thickness" (a int ) and "interfacial permeation resistance" (R int ), are introduced to account for the role of nanotube/matrix interfacial interactions in the proposed model. The obtained values of a int , independent of the nature of the permeate gas, increased by increasing both the nanotubes aspect ratio and polymer-nanotube interfacial strength. An excellent correlation between the values of a int and polymer-nanotube interaction parameters, χ, helped to accurately reproduce the existing experimental data from the literature without the need to resort to any adjustable parameter. The data includes 10 sets of CO 2 /CH 4 permeation, 12 sets of CO 2 /N 2 permeation, 3 sets of CO 2 /O 2 permeation, and 2 sets of CO 2 /H 2 permeation through different nanotube-MMMs. Moreover, the average absolute relative errors between the experimental data and the predicted values of the proposed model are very small (less than 5%) in comparison with those of the existing models in the literature. To the best of our knowledge, this is the first study where such a systematic comparison between model predictions and such extensive experimental data is presented. Finally, the new way of assessing gas permeation data presented in the current work would be a simple alternative to complex approaches that are usually utilized to estimate interfacial thickness in polymer composites.

  14. Double electrolyte sensor for monitoring hydrogen permeation rate in steels

    International Nuclear Information System (INIS)

    Ouyang, Y.J.; Yu, G.; Ou, A.L.; Hu, L.; Xu, W.J.

    2011-01-01

    Highlights: → Designed an amperometric hydrogen sensor with double electrolytes. → Explained the principle of determining hydrogen permeation rate. → Verified good stability, reproducibility and correctness of the developed sensor. → Field on-line monitoring the susceptivity of hydrogen induced cracks. - Abstract: An amperometric hydrogen sensor with double electrolytes composed of a gelatiniform electrolyte and KOH solution has been developed to determine the permeation rate of hydrogen atoms in steel equipment owing to hydrogen corrosion. The gelatiniform electrolyte was made of sodium polyacrylate (PAAS), carboxyl methyl cellulose (CMC) and 0.2 mol dm -3 KOH solution. The results show that the gelatiniform electrolyte containing 50 wt.% polymers has suitable viscosity and high electrical conductivity. The consistent permeation curves were detected by the sensor of the double electrolyte and single liquid KOH electrolyte, respectively. The developed sensor has good stability and reproducibility at room temperature.

  15. Double electrolyte sensor for monitoring hydrogen permeation rate in steels

    Energy Technology Data Exchange (ETDEWEB)

    Ouyang, Y.J. [State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082 (China); Department of Chemistry and Chemical Engineering, Huaihua College, Huaihua 418008 (China); Yu, G., E-mail: yuganghnu@163.co [State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082 (China); Ou, A.L.; Hu, L.; Xu, W.J. [State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082 (China)

    2011-06-15

    Highlights: {yields} Designed an amperometric hydrogen sensor with double electrolytes. {yields} Explained the principle of determining hydrogen permeation rate. {yields} Verified good stability, reproducibility and correctness of the developed sensor. {yields} Field on-line monitoring the susceptivity of hydrogen induced cracks. - Abstract: An amperometric hydrogen sensor with double electrolytes composed of a gelatiniform electrolyte and KOH solution has been developed to determine the permeation rate of hydrogen atoms in steel equipment owing to hydrogen corrosion. The gelatiniform electrolyte was made of sodium polyacrylate (PAAS), carboxyl methyl cellulose (CMC) and 0.2 mol dm{sup -3} KOH solution. The results show that the gelatiniform electrolyte containing 50 wt.% polymers has suitable viscosity and high electrical conductivity. The consistent permeation curves were detected by the sensor of the double electrolyte and single liquid KOH electrolyte, respectively. The developed sensor has good stability and reproducibility at room temperature.

  16. Ion-Exchanged SAPO-34 Membranes for Krypton-Xenon Separation: Control of Permeation Properties and Fabrication of Hollow Fiber Membranes.

    Science.gov (United States)

    Kwon, Yeon Hye; Min, Byunghyun; Yang, Shaowei; Koh, Dong-Yeun; Bhave, Ramesh R; Nair, Sankar

    2018-02-21

    Separation of radioisotope 85 Kr from 136 Xe is of importance in used nuclear fuel reprocessing. Membrane separation based on zeolite molecular sieves such as chabazite SAPO-34 is an attractive alternative to energy-intensive cryogenic distillation. We report the synthesis of SAPO-34 membranes with considerably enhanced performance via thickness reduction based upon control of a steam-assisted vapor-solid conversion technique followed by ion exchange with alkali metal cations. The reduction of membrane thickness leads to a large increase in Kr permeance from 7.5 to 26.3 gas permeation units (GPU) with ideal Kr/Xe selectivities >20 at 298 K. Cation-exchanged membranes show large (>50%) increases in selectivity at ambient or slight subambient conditions. The adsorption, diffusion, and permeation characteristics of ion-exchanged SAPO-34 materials and membranes are investigated in detail, with potassium-exchanged SAPO-34 membranes showing particularly attractive performance. We then demonstrate the fabrication of selective SAPO-34 membranes on α-alumina hollow fibers.

  17. Gas permeation process for post combustion CO2 capture

    International Nuclear Information System (INIS)

    Pfister, Marc

    2017-01-01

    CO 2 Capture and Storage (CCS) is a promising solution to separate CO 2 from flue gas, to reduce the CO 2 emissions in the atmosphere, and hence to reduce global warming. In CCS, one important constraint is the high additional energy requirement of the different capture processes. That statement is partly explained by the low CO 2 fraction in the inlet flue gas and the high output targets in terms of CO 2 capture and purity (≥90%). Gas permeation across dense membrane can be used in post combustion CO 2 capture. Gas permeation in a dense membrane is ruled by a mass transfer mechanism and separation performance in a dense membrane are characterized by component's effective permeability and selectivity. One of the newest and encouraging type of membrane in terms of separation performance is the facilitated transport membrane. Each particular type of membrane is defined by a specific mass transfer law. The most important difference to the mass transfer behavior in a dense membrane is related to the facilitated transport mechanism and the solution diffusion mechanism and its restrictions and limitations. Permeation flux modelling across a dense membrane is required to perform a post combustion CO 2 capture process simulation. A CO 2 gas permeation separation process is composed of a two-steps membrane process, one drying step and a compression unit. Simulation on the energy requirement and surface area of the different membrane modules in the global system are useful to determine the benefits of using dense membranes in a post combustion CO 2 capture technology. (author)

  18. In vitro permeation studies of nanoemulsions containing ketoprofen as a model drug.

    Science.gov (United States)

    Kim, Beom Su; Won, Myoung; Lee, Kang Min; Kim, Cheo Sang

    2008-09-01

    We prepared a nanoemulsion system with benzyl alcohol/ ethanol/Solutol/smash(R) HS 15 /water. Ketoprofen was used as a model drug in this study. The nanoemulsions of this system evidenced a high degree of stability. The droplet diameter did not change over a period of at least 3 months. The nanoemulsion containing 4% benzyl alcohol evidenced a permeation rate higher than was observed with the 1% and 2% nanoemulsions. Also the nanoemulsion containing 1% Solutol(R) HS 15 provided a permeation rate higher than was seen with the 2% and 4% nanoemulsions. All ketoprofen-loaded nanoemulsions enhanced the in vitro permeation rate through mouse skins as compared to the control.

  19. Influence of nitrogen ion implantation on hydrogen permeation in an extra mild steel

    International Nuclear Information System (INIS)

    Brass, A.M.; Chene, J.; Pivin, J.C.

    1989-01-01

    This paper presents the first results on the effect of nitrogen implantation on hydrogen permeation in steels. Nitrogen can modify superficially the steel's chemistry and/or microstructure depending on the fluence and thereby affect the processes of hydrogen diffusion and trapping. The implantations were performed on low carbon steel specimens with different nominal doses (1% to 10% and 33% nitrogen in a superficial layer of approximately 100 to 120 nm). The corresponding microstructures were characterized and permeation tests were conducted at room temperature in a double electrolytic cell. The nitrogen implanted layers on iron affects the electrochemical behaviour of the surface and the permeation in the material. This effect depends on the nitrogen concentration in the layer and on the corresponding microstructure. A continuous Fe 2 N layer acts as an efficient barrier to hydrogen entry and permeation when the layer is located on the entry face of the permeation membrane. This effect is stronger when the implanted layer is on the downstream face of the membrane. The low permeability values are mainly attributed to a lower hydrogen solubility in the implanted layer, whereas hydrogen trapping on defects and nitride precipitates delay hydrogen penetration. (author)

  20. 40 CFR 90.129 - Fuel tank permeation from handheld engines and equipment.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Fuel tank permeation from handheld... KILOWATTS Emission Standards and Certification Provisions § 90.129 Fuel tank permeation from handheld... equipment with respect to fuel tanks. For the purposes of this section, fuel tanks do not include fuel caps...

  1. Ion-driven permeation of deuterium through tungsten under simultaneous helium and deuterium irradiation

    International Nuclear Information System (INIS)

    Lee, H.T.; Tanaka, H.; Ohtsuka, Y.; Ueda, Y.

    2011-01-01

    Ion-driven permeation of D through tungsten under simultaneous irradiation with He-D was measured as a function of temperature, flux, and energy. He reduced the permeation flux with the reduction increasing with decreasing temperature. The reduction in permeation flux followed a linear dependence to the incident flux at T > 1000 K, but shifted to a square root dependence at T < 1000 K. The results were interpreted as shifts from diffusion limited to recombination limited H transport according to Doyle and Brice's theory. Arrhenius functions of front diffusivity and recombination coefficients were derived and used to calculate the transport parameter W. The effect of He can be interpreted as changes to the front diffusivity that approaches H diffusion behavior in the absence of traps. The reduction in total concentration results in a shallower concentration gradient that can describe the observed decrease in permeation.

  2. Hydrogen and deuterium permeation in copper alloys, copper--gold brazing alloys, gold, and the in situ growth of stable oxide permeation barriers

    International Nuclear Information System (INIS)

    Begeal, D.R.

    1978-01-01

    The deuterium permeation through several copper alloys has been measured over a temperature range of 550 to 830 K using the membrane technique. In some cases, the hydrogen permeability was also measured. The results were divided into three categories: common alloys, gold alloys, and stable oxide forming alloys. Common alloys which showed typical bulk metallic diffusion with litle change in the permeation activation energy as compared to copper (77 kJ/mol for D 2 ) were: (additions are in weight percent) 5% Sn, 2.3% U, 0.15% Zr, 4% Sn+4% Pb+4% Zn, 3% Si, and 7% Al+2% Fe. Compared to copper, the D 2 permeability at 573 K was reduced by factors of 2.0, 2.7, 4.5, 5.3, 5.9, and 7.0, respectively. A series of gold--copper alloys including pure gold, 80% Au, 50% Au, 49% Au, and 35% Au also showed typical bulk metallic diffusion with a trend of decreasing permeability (increasing activation energies for permeation) with increasing gold content. There were also pronounced inflections or shifts in the permeability at approx.370 0 C, or about the order--disorder transition for Cu 3 Au and CuAu, for the 80% and 50% alloys. Two alloys did not exhibit bulk metallic permeation behavior and the permeabiltiy was in fact controlled by surface oxide layers. It was found that a layer of beryllium oxide could be formed on Cu+2% Be and a layer of aluminum oxide could be formed on Cu+7% Al+2% Si. As compared to 0.25 mm-thick copper, the deuterium permeability at 500 0 C was reduced by a factor of approx.250 for Cu--Be and approx.1000 for Cu--Al--Si. The activation energies for deuterium permeation were 98 kJ/mol and 132 kJ/mol, respectively. The mechanism for the oxide growth is the high-temperature hydrogen reduction of nearby less stable oxides, simultaneous with oxidation of the active metal, Be or Al, by trace amounts of water in the hydrogen. Ion microprobe mass analysis identified the oxide layers as containing beryllium or aluminum but not containing copper

  3. Hydrogen permeation behavior through F82H at high temperature

    Energy Technology Data Exchange (ETDEWEB)

    Matsuda, S.; Katayama, K.; Shimozori, M.; Fukada, S. [Interdisciplinary Graduate School of Engineering Science, Kyushu University, Kyushu (Japan); Ushida, H. [Energy Science and Engineering, Faculty of Engineering, Kyushu University, Kyushu (Japan); Nishikawa, M. [Malaysia-Japan International Institute of Technology, UTM, Kuala Lumpur (Malaysia)

    2015-03-15

    F82H is a primary candidate of structural material and coolant pipe material in a blanket of a fusion reactor. Understanding tritium permeation behavior through F82H is important. In a normal operation of a fusion reactor, the temperature of F82H will be controlled below 550 C. degrees because it is considered that F82H can be used up to 30,000 hours at 550 C. degrees. However, it is necessary to assume the situation where F82H is heated over 550 C. degrees in a severe accident. In this study, hydrogen permeation behavior through F82H was investigated in the temperature range from 500 to 800 C. degrees. In some cases, water vapor was added in a sample gas to investigate an effect of water vapor on hydrogen permeation. The permeability of hydrogen in the temperature range from 500 to 700 C. degrees agreed well with the permeability reported by E. Serra et al. The degradation of the permeability by water vapor was not observed. After the hydrogen permeation reached in a steady state at 700 C. degrees, the F82H sample was heated to 800 C. degrees. The permeability of hydrogen through F82H sample which was once heated up to 800 C. degrees was lower than that of the original one. (authors)

  4. In vitro permeation through porcine buccal mucosa of Salvia desoleana Atzei & Picci essential oil from topical formulations.

    Science.gov (United States)

    Ceschel, G C; Maffei, P; Moretti, M D; Demontis, S; Peana, A T

    2000-02-15

    In the light of recent studies, which have shown that the essential oil derived from some Lamiaceae species has appreciable anti-inflammatory activity, moderate anti-microbial action and the ability to inhibit induced hyperalgesia, an assessment of the diffusion and permeation of Salvia desoleana Atzei & Picci (S. desoleana) essential oil through porcine buccal mucosa was considered useful for a possible application in the stomatological field. Topical formulations (microemulsions, hydrogels and microemulsion-hydrogels) were prepared for application to the buccal mucosa. The mucosa permeation of the oil from the formulations was evaluated using Franz cells, with porcine buccal mucosa as septum between the formulations (donor compartment) and the receptor phase chambers. The study also aimed at optimising the permeability of the S. desoleana essential oil by means of an enhancer, the diethylene glycol monoethyl ether Transcutol. The diffusion of the oil through the membrane was determined by evaluating the amount of essential oil components present in the receiving solution, the flux and the permeation coefficient (at the steady state) in the different formulations at set intervals. Qualitative and quantitative determinations were done by gas chromatographic analysis. All the formulations allow a high permeability coefficient in comparison with the pure essential oil. In particular, the components with a terpenic structure (beta-pinene, cineole, alpha-terpineol and linalool) have the highest capacity to pass through the porcine buccal mucosa when compared to the other components (linalyl acetate and alpha-terpinil acetate). Moreover, the enhancer, diethylene glycol monoethyl ether largely increases the permeation of the essential oil components in relation to the concentration.

  5. Dual-directional regulation of drug permeating amount by combining the technique of ion-pair complexation with chemical enhancers for the synchronous permeation of indapamide and bisoprolol in their compound patch through rabbit skin.

    Science.gov (United States)

    Song, Wenting; Cun, Dongmei; Quan, Peng; Liu, Nannan; Chen, Yang; Cui, Hongxia; Xiang, Rongwu; Fang, Liang

    2015-04-01

    To achieve the synchronous skin permeation of indapamide (IND) and bisoprolol (BSP) in their compound patch, the techniques of ion-pair complexation and chemical enhancers were combined to dual-directionally regulate drug permeating amounts. Ion-pair complexes of BSP and various organic acids were formed by the technique of ion-pair complexation. Among the complexes formed, bisoprolol tartrate (BSP.T) down-regulated the permeating amount of BSP to the same extent as that of IND. Then, to simultaneously up-regulate the amounts of the two drugs, an enhancer combination of 15.8% Span80 (SP), 6.0% Azone (AZ) and 2.2% N-methyl pyrrolidone (NMP) was obtained by central composite design and exhibited an outstanding and simultaneous enhancement on IND and BSP with enhancing ratio (ER) of 4.52 and 3.49, respectively. The effect of the dual-directional regulation was evaluated by in vitro permeation experiments and in vivo pharmacokinetic studies. For IND and BSP, their observed permeation profiles were comparable and their MAT (mean absorption time) showed no significant difference, which both demonstrated these two drugs achieved the synchronous skin permeation in their compound patch by the dual-directional regulation strategy of combining the technique of ion-pair complexation with chemical enhancers. Copyright © 2015 Elsevier B.V. All rights reserved.

  6. Human skin in vitro permeation of bentazon and isoproturon formulations with or without protective clothing suit.

    Science.gov (United States)

    Berthet, Aurélie; Hopf, Nancy B; Miles, Alexandra; Spring, Philipp; Charrière, Nicole; Garrigou, Alain; Baldi, Isabelle; Vernez, David

    2014-01-01

    Skin exposures to chemicals may lead, through percutaneous permeation, to a significant increase in systemic circulation. Skin is the primary route of entry during some occupational activities, especially in agriculture. To reduce skin exposures, the use of personal protective equipment (PPE) is recommended. PPE efficiency is characterized as the time until products permeate through material (lag time, Tlag). Both skin and PPE permeations are assessed using similar in vitro methods; the diffusion cell system. Flow-through diffusion cells were used in this study to assess the permeation of two herbicides, bentazon and isoproturon, as well as four related commercial formulations (Basagran(®), Basamais(®), Arelon(®) and Matara(®)). Permeation was measured through fresh excised human skin, protective clothing suits (suits) (Microchem(®) 3000, AgriSafe Pro(®), Proshield(®) and Microgard(®) 2000 Plus Green), and a combination of skin and suits. Both herbicides, tested by itself or as an active ingredient in formulations, permeated readily through human skin and tested suits (Tlag < 2 h). High permeation coefficients were obtained regardless of formulations or tested membranes, except for Microchem(®) 3000. Short Tlag, were observed even when skin was covered with suits, except for Microchem(®) 3000. Kp values tended to decrease when suits covered the skin (except when Arelon(®) was applied to skin covered with AgriSafe Pro and Microgard(®) 2000), suggesting that Tlag alone is insufficient in characterizing suits. To better estimate human skin permeations, in vitro experiments should not only use human skin but also consider the intended use of the suit, i.e., the active ingredient concentrations and type of formulations, which significantly affect skin permeation.

  7. Deuterium permeation of amorphous alumina coating on 316L prepared by MOCVD

    International Nuclear Information System (INIS)

    Li Shuai; He Di; Liu Xiaopeng; Wang Shumao; Jiang Lijun

    2012-01-01

    Highlights: ► Deuterium permeation behavior of alumina coating by MOCVD is investigated. ► The as-prepared alumina is amorphous. ► The alumina coating is dense and well adherent to substrate. ► Deuterium permeation rate of alumina coating is 2–3 orders of magnitude lower than martensitic steels. - Abstract: The deuterium permeation behavior of the alumina coating on 316L stainless steel prepared by metal organic chemical vapor deposition (MOCVD) was investigated. The alumina coating was also characterized by X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and scanning electron microscope (SEM). It was found that the as-prepared coating consisted of amorphous alumina. This alumina coating had a dense, crack-free and homogeneous morphology. Although the alumina coating was amorphous, effective suppression of deuterium permeation was demonstrated. The deuterium permeability of the alumina coating was 51–60 times less than that of the 316L stainless steel and 153–335 times less than that of the referred low activation martensitic steels at 860–960 K.

  8. Comparative study of silver nanoparticle permeation using Side-Bi-Side and Franz diffusion cells

    Science.gov (United States)

    Trbojevich, Raul A.; Fernandez, Avelina; Watanabe, Fumiya; Mustafa, Thikra; Bryant, Matthew S.

    2016-03-01

    Better understanding the mechanisms of nanoparticle permeation through membranes and packaging polymers has important implications for the evaluation of drug transdermal uptake, in food safety and the environmental implications of nanotechnology. In this study, permeation of 21 nm diameter silver nanoparticles (AgNPs) was tested using Side-Bi-Side and Franz static diffusion cells through hydrophilic 0.1 and 0.05 µm pore diameter 125 µm thick synthetic cellulose membranes, and 16 and 120 µm thick low-density polyethylene (LDPE) films. Experiments performed with LDPE films discarded permeation of AgNPs or Ag ions over the investigated time-frame in both diffusion systems. But controlled release of AgNPs has been quantified using semipermeable hydrophilic membranes. The permeation followed a quasi-linear time-dependent model during the experimental time-frame, which represents surface reaction-limited permeation. Diffusive flux, diffusion coefficients, and membrane permeability were determined as a function of pore size and diffusion model. Concentration gradient and pore size were key to understand mass transfer phenomena in the diffusion systems.

  9. Comparative study of silver nanoparticle permeation using Side-Bi-Side and Franz diffusion cells

    International Nuclear Information System (INIS)

    Trbojevich, Raul A.; Fernandez, Avelina; Watanabe, Fumiya; Mustafa, Thikra; Bryant, Matthew S.

    2016-01-01

    Better understanding the mechanisms of nanoparticle permeation through membranes and packaging polymers has important implications for the evaluation of drug transdermal uptake, in food safety and the environmental implications of nanotechnology. In this study, permeation of 21 nm diameter silver nanoparticles (AgNPs) was tested using Side-Bi-Side and Franz static diffusion cells through hydrophilic 0.1 and 0.05 µm pore diameter 125 µm thick synthetic cellulose membranes, and 16 and 120 µm thick low-density polyethylene (LDPE) films. Experiments performed with LDPE films discarded permeation of AgNPs or Ag ions over the investigated time-frame in both diffusion systems. But controlled release of AgNPs has been quantified using semipermeable hydrophilic membranes. The permeation followed a quasi-linear time-dependent model during the experimental time-frame, which represents surface reaction-limited permeation. Diffusive flux, diffusion coefficients, and membrane permeability were determined as a function of pore size and diffusion model. Concentration gradient and pore size were key to understand mass transfer phenomena in the diffusion systems.Graphical Abstract

  10. Comparative study of silver nanoparticle permeation using Side-Bi-Side and Franz diffusion cells

    Energy Technology Data Exchange (ETDEWEB)

    Trbojevich, Raul A. [U.S. Food and Drug Administration, Division of Biochemical Toxicology, National Center for Toxicological Research (United States); Fernandez, Avelina, E-mail: velifdez@ific.uv.es [Consejo Superior de Investigaciones Científicas-Universitat de València, Parc Científic, Instituto de Física Corpuscular (Spain); Watanabe, Fumiya; Mustafa, Thikra [University Arkansas at Little Rock, Center for Integrative Nanotechnology Sciences (United States); Bryant, Matthew S. [U.S. Food and Drug Administration, Division of Biochemical Toxicology, National Center for Toxicological Research (United States)

    2016-03-15

    Better understanding the mechanisms of nanoparticle permeation through membranes and packaging polymers has important implications for the evaluation of drug transdermal uptake, in food safety and the environmental implications of nanotechnology. In this study, permeation of 21 nm diameter silver nanoparticles (AgNPs) was tested using Side-Bi-Side and Franz static diffusion cells through hydrophilic 0.1 and 0.05 µm pore diameter 125 µm thick synthetic cellulose membranes, and 16 and 120 µm thick low-density polyethylene (LDPE) films. Experiments performed with LDPE films discarded permeation of AgNPs or Ag ions over the investigated time-frame in both diffusion systems. But controlled release of AgNPs has been quantified using semipermeable hydrophilic membranes. The permeation followed a quasi-linear time-dependent model during the experimental time-frame, which represents surface reaction-limited permeation. Diffusive flux, diffusion coefficients, and membrane permeability were determined as a function of pore size and diffusion model. Concentration gradient and pore size were key to understand mass transfer phenomena in the diffusion systems.Graphical Abstract.

  11. Modell experiments to determine the effect of inhibitive oxide layers on metals against hydrogen permeation

    International Nuclear Information System (INIS)

    Zink, U.

    1983-11-01

    The coupling of H 2 -permeation and corrosion has been examined with the high-temperature alloys Incoloy 800 and Incoloy 802. Permeationsrates as well as corrosionsrates have been measured simultanously under H 2 O-H 2 atmospheres in the test-facility HD-PERM. Test parameters have been temperature and oxidationpotential. Parabolic laws for the growth of the oxide scales have been identified and are considered to be highly important for the efficiency of a permeation barrier. A comparison between the temperature dependencies of corrosionsrates and H 2 -permeationsrates has revealed that permeation and corrosion are coupled only in so far that the permeation barrier is formed by the corrosion reaction. The corrosion data (parabolic rate constant, activation energy) of the oxide scales have given clear indications for the existence of a Cr 2 O 3 -layer, which is considered to be responsible for efficient oxide permeation barriers. (orig.) [de

  12. Enhanced skin permeation of naltrexone by pulsed electromagnetic fields in human skin in vitro.

    Science.gov (United States)

    Krishnan, Gayathri; Edwards, Jeffrey; Chen, Yan; Benson, Heather A E

    2010-06-01

    The aim of the present study was to evaluate the skin permeation of naltrexone (NTX) under the influence of a pulsed electromagnetic field (PEMF). The permeation of NTX across human epidermis and a silicone membrane in vitro was monitored during and after application of the PEMF and compared to passive application. Enhancement ratios of NTX human epidermis permeation by PEMF over passive diffusion, calculated based on the AUC of cumulative NTX permeation to the receptor compartment verses time for 0-4 h, 4-8 h, and over the entire experiment (0-8 h) were 6.52, 5.25, and 5.66, respectively. Observation of the curve indicated an initial enhancement of NTX permeation compared to passive delivery whilst the PEMF was active (0-4 h). This was followed by a secondary phase after termination of PEMF energy (4-8 h) in which there was a steady increase in NTX permeation. No significant enhancement of NTX penetration across silicone membrane occurred with PEMF application in comparison to passively applied NTX. In a preliminary experiment PEMF enhanced the penetration of 10 nm gold nanoparticles through the stratum corneum as visualized by multiphoton microscopy. This suggests that the channels through which the nanoparticles move must be larger than the 10 nm diameter of these rigid particles. (c) 2009 Wiley-Liss, Inc. and the American Pharmacists Association

  13. 40 CFR 1060.102 - What permeation emission control requirements apply for fuel lines?

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 32 2010-07-01 2010-07-01 false What permeation emission control... AGENCY (CONTINUED) AIR POLLUTION CONTROLS CONTROL OF EVAPORATIVE EMISSIONS FROM NEW AND IN-USE NONROAD AND STATIONARY EQUIPMENT Emission Standards and Related Requirements § 1060.102 What permeation...

  14. 40 CFR 1060.103 - What permeation emission control requirements apply for fuel tanks?

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 32 2010-07-01 2010-07-01 false What permeation emission control... AGENCY (CONTINUED) AIR POLLUTION CONTROLS CONTROL OF EVAPORATIVE EMISSIONS FROM NEW AND IN-USE NONROAD AND STATIONARY EQUIPMENT Emission Standards and Related Requirements § 1060.103 What permeation...

  15. Permeation of hydrogen at low pressures through stainless steel and implications for tritium control in fusion reactor systems

    International Nuclear Information System (INIS)

    Axtmann, R.C.; Johnson, E.F.; Kuehler, C.W.

    1976-01-01

    New experimental data on the permeation of hydrogen through stainless steel indicate that at driving pressures below 10 -2 torr, the permeation rate is linearly dependent on the driving pressure. A possible consequence is that the permeation rates of hydrogenic species in fusion reactor systems might be much lower than those reported in contemporary conceptual design studies which assume that the rates are dependent on the square root of the driving pressure. The important implications of these low permeation rates are: (1) tritium losses to the environment may be more dependent on ordinary leaks from equipment than on permeation to the steam cycle; (2) recovery of tritium from breeding blankets via permeation windows may be impracticable; and (3) recovery of tritium from breeding blankets not dependent on permeation windows may be simplified by the possibility of operating at much higher average tritium concentrations in the blanket and cooling systems

  16. Effect of pressure sensitive adhesive and vehicles on permeation of terbinafine across porcine hoof membrane.

    Science.gov (United States)

    Ahn, Tai Sang; Lee, Jung-Phil; Kim, Juhyun; Oh, Seaung Youl; Chun, Myung-Kwan; Choi, Hoo-Kyun

    2013-11-01

    The purpose of this study was to investigate characteristics of transungual drug delivery and the feasibility of developing a drug-in-adhesive formulation of terbinafine. The permeation of terbinafine from a PSA matrix across porcine hoof membrane was determined using a plate containing poloxamer gel. The permeation rate of terbinafine across hairless mouse skin was evaluated using a flow-through diffusion cell system. The permeation of terbinafine across the hoof membranes was the highest from the silicone adhesive matrix, followed by PIB, and most of the acrylic adhesives, SIS, and SBS. The rank order of permeation rate across mice skin was different from the rank order across porcine hooves. The amount of terbinafine permeated across the porcine hoof membranes poorly correlated with the amount of terbinafine remaining inside the hooves after 20 days, however, the ratio between rate of terbinafine partitioning into the hoof membrane and its rate of diffusion across the membrane was relatively constant within the same type of PSA. For influence of various vehicles in enhancing permeation of terbinafine across the hoof membrane, all vehicles except Labrasol(®) showed tendency to improve permeation rate. However, the enhancement ratio of a given vehicle differed from one adhesive to another with a moderate correlation between them. The infrared spectrum of the hoof treated with NMP, PPG 400 or PEG 200 indicated that the conformation of keratin changed from a non-helical to a helical structure.

  17. Resistance of gloves and protective clothing materials to permeation of cytostatic solutions.

    Science.gov (United States)

    Krzemińska, Sylwia; Pośniak, Małgorzata; Szewczyńska, Małgorzata

    2018-01-15

    The objective of the work was to determine the resistance of selected protective clothing and glove materials to permeation of cytostatics such as docetaxel, fluorouracil, and doxorubicin. The following glove materials were used: natural rubber latex (code A), acrylonitrile-butadiene rubber (code B) and chloroprene rubber (code C). In addition, we tested a layered material composed of a non-woven polyester (PES), a polypropylene (PP) film, and a non-woven PP used for protective coats (code D). The cytostatics were analyzed by liquid chromatography with diode array detection. The tested samples were placed in a purpose-built permeation cell modified to be different from that specified in the standard EN 6529:2001. The tested materials were characterized by good resistance to solutions containing 2 out of the 3 selected cytostatics: doxorubicin and 5-fluorouracil, as indicated by a breakthrough time of over 480 min. Equally high resistance to permeation of the third cytostatic (docetaxel) was exhibited by natural rubber latex, acrylonitrile-butadiene rubber, and chloroprene rubber. However, docetaxel permeated much more readily through the clothing layered material, compromising its barrier properties. It was found that the presence of additional components in cytostatic preparations accelerated permeation through material samples, thus deteriorating their barrier properties. Int J Occup Med Environ Health 2018;31(3):341-350. This work is available in Open Access model and licensed under a CC BY-NC 3.0 PL license.

  18. Whole glove permeation of cyclohexanol through disposable nitrile gloves on a dextrous robot hand: Fist clenching vs. non-clenching.

    Science.gov (United States)

    Mathews, Airek R; Que Hee, Shane S

    2017-04-01

    The differences in permeation parameters when a gloved dextrous robot hand clenched and did not were investigated with the dynamic permeation system described in the companion paper. Increased permeation through the gloves of the present study for cyclohexanol when the gloved hand clenched depended on glove thickness and porosity for cyclohexanol permeation. The Sterling glove, the thinnest and most porous, was the least protective. Hand clenching promoted more permeation for the Sterling glove in terms of breakthrough times, steady state permeation rate, and diffusion coefficient. The Safeskin glove showed increased permeation only for the steady state permeation rate but not breakthrough times or diffusion coefficient. The Blue and Purple gloves showed no differences when the hand was clenching or not. The correlational analysis supported differences between the clenching and non-clenching situations, and the risk assessment considered the worst and best scenarios relative to one and two hydrated hands that were and were not protected by specific gloves.

  19. Optimisation of cosolvent concentration for topical drug delivery III--influence of lipophilic vehicles on ibuprofen permeation.

    Science.gov (United States)

    Watkinson, R M; Guy, R H; Oliveira, G; Hadgraft, J; Lane, M E

    2011-01-01

    Previously, we have reported the effects of water, ethanol, propylene glycol and various binary and ternary mixtures of these solvents on the permeation of ibuprofen in model membranes and in skin. The present study investigates the influence of lipophilic vehicles on the transport of ibuprofen in silicone membrane and in human skin. The permeation of ibuprofen was measured from mineral oil (MO), Miglyol® 812 (MG) and binary mixtures of MO and MG. The solubility of ibuprofen was 5-fold higher in MG than in MO, however, the permeation of ibuprofen from the pure vehicles and combinations of both was comparable in silicone membrane. Additionally, there were no significant differences in skin permeation for MO and MG vehicles. When the permeation of various hydrophilic and lipophilic vehicles is considered, a trend between flux values for the model membrane and skin is evident (r(2) = 0.71). The findings suggest that silicone membrane may provide information on qualitative trends in skin permeation for vehicles of diverse solubility and partition characteristics. Copyright © 2010 S. Karger AG, Basel.

  20. Implantation measurements to determine tritium permeation in first wall structures

    International Nuclear Information System (INIS)

    Holland, D.F.; Causey, R.A.

    1983-01-01

    A principal safety concern for a D-T burning fusion reactor is release of tritium during routine operation. Tritium implantation into first wall structures, and subsequent permeation into coolants, is potentially an important source of tritium loss. This paper reports on an experiment in which an ion accelerator was used to implant deuterium atoms in a stainless steel disk to simulate tritium implantation in first wall structures. The permeation rate was measured under various operating conditions. These results were used in the TMAP computer code to determine potential tritium loss rates for fusion reactors

  1. Implantation measurements to determine tritium permeation in first-wall structures

    International Nuclear Information System (INIS)

    Holland, D.F.; Causey, R.A.; Sattler, M.L.

    1983-01-01

    A principal safety concern for a D-T burning fusion reactor is release of tritium during routine operation. Tritium implantation into first-wall structures, and subsequent permeation into coolants, is potentially an important source of tritium loss. This paper reports on an experiment in which an ion accelerator was used to implant deuterium atoms in a stainless steel disk to simulate tritium implantation in first-wall structures. The permeation rate was measured under various operating conditions. These results were used in the TMAP computer code to determine potential tritium loss rates for fusion reactors

  2. Effects of Vehicles and Enhancers on the Skin Permeation of Phytoestrogenic Diarylheptanoids from Curcuma comosa.

    Science.gov (United States)

    Tuntiyasawasdikul, Sarunya; Limpongsa, Ekapol; Jaipakdee, Napaphak; Sripanidkulchai, Bungorn

    2017-04-01

    Curcuma comosa (C. comosa) is widely used in traditional medicine as a dietary supplement for health promotion in postmenopausal women in Thailand. It contains several diarylheptanoids, which are considered to be a novel class of phytoestrogens. However, the diarylheptanoids isolated from the plant rhizome are shown to have low oral bioavailability and faster elimination characteristics. The aim of this study was to investigate the permeation behavior of the active compounds of diarylheptanoids. The effects of binary vehicle systems and permeation enhancers on diarylheptanoids permeation and accumulation within the skin were studied using side-by-side diffusion cells through the porcine ear skin. Among the tested binary vehicle systems, the ethanol/water vehicle appeared to be the most effective system for diarylheptanoids permeation with the highest flux and shortest lag time. The presence of transcutol in the vehicle system significantly increased diarylheptanoid's permeation and accumulation within the skin in a concentration-dependent manner. Although the presence of terpenes in formulation decreased the flux of diarylheptanoids, it raised the amount of diarylheptanoids retained within the skin substantially. Based on the feasibility of diarylheptanoid permeation, C. comosa extract should be further developed into an effective transdermal product for health benefits and hormone replacement therapy.

  3. Skin permeation of D-limonene-based nanoemulsions as a transdermal carrier prepared by ultrasonic emulsification.

    Science.gov (United States)

    Lu, Wen-Chien; Chiang, Been-Huang; Huang, Da-Wei; Li, Po-Hsien

    2014-03-01

    Nanoemulsions can be used for transporting pharmaceutical phytochemicals in skin-care products because of their stability and rapid permeation properties. However, droplet size may be a critical factor aiding permeation through skin and transdermal delivery efficiency. We prepared D-limonene nanoemulsions with various droplet sizes by ultrasonic emulsification using mixed surfactants of sorbitane trioleate and polyoxyethylene (20) oleyl ether under different hydrophilic-lipophilic balance (HLB) values. Droplet size decreased with increasing HLB value. With HLB 12, the droplet size was 23 nm, and the encapsulated ratio peaked at 92.3%. Transmission electron microscopy revealed spherical droplets and the gray parts were D-limonene precipitation incorporated in spherical droplets of the emulsion system. Franz diffusion cell was used to evaluate the permeation of D-limonene nanoemulsion through rat abdominal skin; the permeation rate depended on droplet size. The emulsion with the lowest droplet size (54 nm) achieved the maximum permeation rate. The concentration of D-limonene in the skin was 40.11 μL/cm(2) at the end of 360 min. Histopathology revealed no distinct voids or empty spaces in the epidermal region of permeated rat skin, so the D-limonene nanoemulsion may be a safe carrier for transdermal drug delivery. Copyright © 2013 Elsevier B.V. All rights reserved.

  4. Intact penetratin metabolite permeates across Caco-2 monolayers

    DEFF Research Database (Denmark)

    Birch, Ditlev; Christensen, Malene Vinther; Stærk, Dan

    . Previous studies have demonstrated that cell-penetrating peptides (CPPs) may be used as carriers in order to improve the bioavailability of a therapeutic cargo like insulin after oral administration. Penetratin, a commonly used CPP, has been shown to increase the uptake of insulin across Caco-2 cell......-2 cells cultured on permeable filter inserts and in cell lysates, respectively. The epithelial permeation of penetratin and the formed metabolites was assessed by using Caco-2 monolayers cultured on permeable filter inserts. Results Preliminary data revealed that at least one specific metabolite...... is formed upon both intracellular and extracellular degradation of penetratin (figure 1A). Following incubation with epithelium for 4 hours, the metabolite permeated the Caco-2 monolayer and the concentration increased approximately 10-fold when compared to a sample collected following 15 minutes...

  5. Gas Permeation Characteristics across Nano-Porous Inorganic Membranes

    Directory of Open Access Journals (Sweden)

    M.R Othman, H. Mukhtar

    2012-10-01

    Full Text Available An overview of parameters affecting gas permeation in inorganic membranes is presented. These factors include membrane physical characteristics, operational parameters and gas molecular characteristics. The membrane physical characteristics include membrane materials and surface area, porosity, pore size and pore size distribution and membrane morphology. The operational parameters include feed flow rate and concentration, stage cut, temperature and pressure. The gas molecular characteristics include gas molecular weight, diameter, critical temperature, critical pressure, Lennard-Jones parameters and diffusion volumes. The current techniques of material characterization may require complementary method in describing microscopic heterogeneity of the porous ceramic media. The method to be incorporated in the future will be to apply a stochastic model and/or fractal dimension. Keywords: Inorganic membrane, surface adsorption, Knudsen diffusion, Micro-porous membrane, permeation, gas separation.

  6. Permeation of antigen protein-conjugated nanoparticles and live bacteria through microneedle-treated mouse skin

    Science.gov (United States)

    Kumar, Amit; Li, Xinran; Sandoval, Michael A; Rodriguez, B Leticia; Sloat, Brian R; Cui, Zhengrong

    2011-01-01

    Background: The present study was designed to evaluate the extent to which pretreatment with microneedles can enhance skin permeation of nanoparticles in vitro and in vivo. Permeation of live bacteria, which are physically nanoparticles or microparticles, through mouse skin pretreated with microneedles was also studied to evaluate the potential risk of microbial infection. Methods and results: It was found that pretreatment of mouse skin with microneedles allowed permeation of solid lipid nanoparticles, size 230 nm, with ovalbumin conjugated on their surface. Transcutaneous immunization in a mouse skin area pretreated with microneedles with ovalbumin nanoparticles induced a stronger antiovalbumin antibody response than using ovalbumin alone. The dose of ovalbumin antigen determined whether microneedle-mediated transcutaneous immunization with ovalbumin nanoparticles induced a stronger immune response than subcutaneous injection of the same ovalbumin nanoparticles. Microneedle treatment permitted skin permeation of live Escherichia coli, but the extent of the permeation was not greater than that enabled by hypodermic injection. Conclusion: Transcutaneous immunization on a microneedle-treated skin area with antigens carried by nanoparticles can potentially induce a strong immune response, and the risk of bacterial infection associated with microneedle treatment is no greater than that with a hypodermic injection. PMID:21753877

  7. Transcriptome Analysis of Three Sheep Intestinal Regions reveals Key Pathways and Hub Regulatory Genes of Large Intestinal Lipid Metabolism.

    Science.gov (United States)

    Chao, Tianle; Wang, Guizhi; Ji, Zhibin; Liu, Zhaohua; Hou, Lei; Wang, Jin; Wang, Jianmin

    2017-07-13

    The large intestine, also known as the hindgut, is an important part of the animal digestive system. Recent studies on digestive system development in ruminants have focused on the rumen and the small intestine, but the molecular mechanisms underlying sheep large intestine metabolism remain poorly understood. To identify genes related to intestinal metabolism and to reveal molecular regulation mechanisms, we sequenced and compared the transcriptomes of mucosal epithelial tissues among the cecum, proximal colon and duodenum. A total of 4,221 transcripts from 3,254 genes were identified as differentially expressed transcripts. Between the large intestine and duodenum, differentially expressed transcripts were found to be significantly enriched in 6 metabolism-related pathways, among which PPAR signaling was identified as a key pathway. Three genes, CPT1A, LPL and PCK1, were identified as higher expression hub genes in the large intestine. Between the cecum and colon, differentially expressed transcripts were significantly enriched in 5 lipid metabolism related pathways, and CEPT1 and MBOAT1 were identified as hub genes. This study provides important information regarding the molecular mechanisms of intestinal metabolism in sheep and may provide a basis for further study.

  8. Deuterium gas-driven permeation and subsequent retention in rolled tungsten foils

    International Nuclear Information System (INIS)

    Liu, Feng; Zhou, Haishan; Li, Xiao-Chun; Xu, Yuping; An, Zhongqing; Mao, Hongmin; Xing, Wenjing; Hou, Qing; Luo, Guang-Nan

    2014-01-01

    Experiments concerning deuterium gas-driven permeation through rolled tungsten foils in the temperature range of 850–950 K and subsequent deuterium retention have been performed. The steady state permeation flux of deuterium is proportional to the square root of the driving pressure. The permeability of deuterium is in an order of 10 −14 mol m −1 s −1 Pa −1/2 in this temperature range and the activation energy for permeation is 1.21 eV. Measurements of diffusivity are significantly affected by the driving pressure, which can be well explained by a saturable-trap model. Thermal desorption spectra of samples feature a single deuterium release peak at about 873 K. TMAP 4 modeling of this peak gives a detrapping energy of 1.70 eV, which fits the dissociation enthalpy of deuterium desorbing from the inner wall of vacancy clusters or pores in tungsten

  9. Deuterium gas-driven permeation and subsequent retention in rolled tungsten foils

    Science.gov (United States)

    Liu, Feng; Zhou, Haishan; Li, Xiao-Chun; Xu, Yuping; An, Zhongqing; Mao, Hongmin; Xing, Wenjing; Hou, Qing; Luo, Guang-Nan

    2014-12-01

    Experiments concerning deuterium gas-driven permeation through rolled tungsten foils in the temperature range of 850-950 K and subsequent deuterium retention have been performed. The steady state permeation flux of deuterium is proportional to the square root of the driving pressure. The permeability of deuterium is in an order of 10-14 mol m-1 s-1 Pa-1/2 in this temperature range and the activation energy for permeation is 1.21 eV. Measurements of diffusivity are significantly affected by the driving pressure, which can be well explained by a saturable-trap model. Thermal desorption spectra of samples feature a single deuterium release peak at about 873 K. TMAP 4 modeling of this peak gives a detrapping energy of 1.70 eV, which fits the dissociation enthalpy of deuterium desorbing from the inner wall of vacancy clusters or pores in tungsten.

  10. Experimental evaluation of tritium permeation through stainless steel tubes of heat exchanger from primary to secondary water in ITER

    International Nuclear Information System (INIS)

    Nakamura, Hirofumi; Nishi, Masataka

    2004-01-01

    Tritium permeation through heat exchanger from primary cooling water to secondary cooling water has been investigated experimentally with SS316L heat exchanger under simulated ITER (international thermonuclear experimental reactor) operation condition in order to establish the tritium permeation evaluation method through the heat exchanger. As the result, the permeation rate of aqueous tritium was found to be about three orders smaller than that of the gaseous tritium. Tritium permeation through the heat exchanger in ITER was then evaluated, and it was revealed that total tritium permeation amount based on obtained aqueous permeability was about one order less than that with the former method with the gaseous permeability and putting the permeation reduction factor as 1000. Evaluated tritium permeation amount into secondary water during 20 years was quite small, which could be considered as negligible from the safety viewpoint

  11. Combined use of bile acids and aminoacids to improve permeation properties of acyclovir.

    Science.gov (United States)

    Cirri, M; Maestrelli, F; Mennini, N; Mura, P

    2015-07-25

    The aim of this work was to develop a topical formulation with improved permeation properties of acyclovir. Ursodeoxycholic (UDC) and dehydrocholic (DHC) acids were tested as potential enhancers, alone or in combination with different aminoacids. Equimolar binary and ternary systems of acyclovir with cholic acids and basic, hydrophilic or hydrophobic aminoacids were prepared by co-grinding in a high vibrational micromill. Differential scanning calorimetry (DSC) was used to characterize the solid state of these systems, while their permeation properties were evaluated in vitro through a lipophilic artificial membrane. UDC was more than 2 times more effective than DHC in improving drug AUC and permeation rate. As for the ternary systems drug-UDC-aminoacid, only the combined use of l-lysine with UDC acid produced an evident synergistic effect in enhancing drug permeation properties, enabling an almost 3 and 8 times AUC increase compared to the binary UDC system or the pure drug, respectively. The best systems were selected for the development of topical cream formulations, adequately characterized and tested for in vitro drug permeation properties and stability on storage. The better performance revealed by acyclovir-UDC-l-lysine was mainly attributed to the formation of a more permeable activated system induced by the multicomponent co-grinding process. Copyright © 2015 Elsevier B.V. All rights reserved.

  12. Influence of bulk and surface phenomena on the hydrogen permeation through metals

    International Nuclear Information System (INIS)

    Waelbroeck, F.; Wienhold, P.; Winter, J.; Rota, E.; Bauno, T.

    1984-12-01

    We discuss the permeation of hydrogen through metals and alloys such as iron, nickel, steels and Inconel wherein H dissolves endothermically from an H 2 gas. We assume first that trapping centers, surface contamination layers, the saturation of the H surface coverage and the implantation profile - when energetic ions drive the permeation - can be neglected, that a quasi-equilibrium exists between the H atom concentration ν in the adsorbed layer and c in the near surface layers and that the H solubility and diffusivity are homogeneous in the membrane. We evaluate thereafter separately the influence of these various effects and identify the parameter domains where appreciable corrections result. The permeation phenomenon is complex even when these simplifications are made: the penetration rate is proportional to the flux of thermal molecules, atoms or energetic ions - depending upon the case - which strike the surface; the diffusion in the metal is proportional to the gradient of c; the release rate depends on c 2 ; the time-dependent diffusion equation includes a double spatial derivative of c. Permeation can only be fully described when computer codes such as PERI is used. Simple analytical relations are however obtained in several limiting cases. They are the object of this report. Some of them had already been derived by other authors but they were not shown to be part of a single, self consistent permeation model. A comparison of predicted and experimental results shows that the simplified model describes surprisingly accurately the hydrogen exchange between gas and metal solutions. (orig./GSCH)

  13. Catalytic reforming of methane to syngas in an oxygen-permeative membrane reactor

    Energy Technology Data Exchange (ETDEWEB)

    Urano, Takeshi; Kubo, Keiko; Saito, Tomoyuki; Hitomi, Atsushi, E-mail: turano@jp.tdk.com [Materials and Process Development Center, TDK Corporation 570-2, Matsugashita, Minamihatori, Narita, Chiba 286-8588 (Japan)

    2011-05-15

    For fuel cell applications, partial oxidative reforming of methane to syngas, hydrogen and carbon monoxide, was performed via a dense oxygen-permeative ceramic membrane composed by both ionic and electronic conductive materials. The modification of Ni-based catalyst by noble metals was investigated to increase oxygen permeation flux and decrease carbon deposition during reforming reaction. The role of each component in catalyst was also discussed.

  14. 40 CFR 1060.520 - How do I test fuel tanks for permeation emissions?

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 32 2010-07-01 2010-07-01 false How do I test fuel tanks for... STATIONARY EQUIPMENT Test Procedures § 1060.520 How do I test fuel tanks for permeation emissions? Measure permeation emissions by weighing a sealed fuel tank before and after a temperature-controlled soak. (a...

  15. Production of citric acid from whey permeate by fermentation using Aspergillus niger

    Energy Technology Data Exchange (ETDEWEB)

    Hossain, M; Brooks, J D

    1983-08-01

    The use of lactic casein whey permeate as a substrate for citric acid production by fermentation has been investigated. Using a mutant strain of Aspergillus niger IMI 41874 in fermenter culture, a citric acid concentration of 8.3 g/l, representing a yield of 19% (w/w) based on lactose utilized, has been observed. Supplementation of the permeate with lactose (final concentration 140 g/l) increased the production to 14.8 g/l (yield 23%). The natural pH of the permeate (pH 4.5) was the most suitable initial pH for the process, and pH control during the fermentation was unnecessary. The addition of methanol (final concentration 3% v/v) to the fermentation increased the citric acid production to 25 g/l (yield 33%, based on lactose utilized). 13 references.

  16. Random sampling of elementary flux modes in large-scale metabolic networks.

    Science.gov (United States)

    Machado, Daniel; Soons, Zita; Patil, Kiran Raosaheb; Ferreira, Eugénio C; Rocha, Isabel

    2012-09-15

    The description of a metabolic network in terms of elementary (flux) modes (EMs) provides an important framework for metabolic pathway analysis. However, their application to large networks has been hampered by the combinatorial explosion in the number of modes. In this work, we develop a method for generating random samples of EMs without computing the whole set. Our algorithm is an adaptation of the canonical basis approach, where we add an additional filtering step which, at each iteration, selects a random subset of the new combinations of modes. In order to obtain an unbiased sample, all candidates are assigned the same probability of getting selected. This approach avoids the exponential growth of the number of modes during computation, thus generating a random sample of the complete set of EMs within reasonable time. We generated samples of different sizes for a metabolic network of Escherichia coli, and observed that they preserve several properties of the full EM set. It is also shown that EM sampling can be used for rational strain design. A well distributed sample, that is representative of the complete set of EMs, should be suitable to most EM-based methods for analysis and optimization of metabolic networks. Source code for a cross-platform implementation in Python is freely available at http://code.google.com/p/emsampler. dmachado@deb.uminho.pt Supplementary data are available at Bioinformatics online.

  17. LIBRETTO-3: Performance of tritium permeation barriers under irradiation at the HFR Petten

    International Nuclear Information System (INIS)

    Conrad, R.; Fuetterer, M.A.; Giancarli, L.; May, R.; Perujo, A.; Sample, T.

    1994-01-01

    The LIBRETTO-3 irradiation was performed at the HFR Petten during 77 full power days in three cycles to compare the efficiency of three different tritium permeation barriers in presence of Pb-17Li to uncoated AISI 316L steel. For this purpose four steel capsules (arnothing o =10 mm, arnothing i =8 mm, l=300 mm) were filled with 28 g Pb-17Li. The coatings included CVD TiC (outside), CVD TiC+Al 2 O 3 (inside), and pack cementation aluminisation (inside). The generated tritium was partly extracted by bubbling, partly it permeated through the capsules. Permeated and extracted tritium were measured as a function of temperature (280-450 C), H 2 doping (0-1 vol%) and purge gas flow rate. The driving partial pressure in the coated capsules were from an extraction model calibrated by the uncoated capsule for which tritium partial pressure could be calculated. In LIBRETTO-3 conditons, the best barrier was pack cementation aluminisation. The first interpretation of the experiment could, however, not confirm permeation reduction factors of 100 or more expected from this barrier. ((orig.))

  18. Deuterium gas-driven permeation and subsequent retention in rolled tungsten foils

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Feng [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031 (China); Zhou, Haishan [Department of Fusion Science, The Graduate University for Advanced Studies, Toki 509-5292 (Japan); Li, Xiao-Chun [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031 (China); Xu, Yuping; An, Zhongqing [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031 (China); School of Nuclear Science and Technology, University of Science and Technology of China, Hefei 230026 (China); Mao, Hongmin; Xing, Wenjing [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031 (China); Hou, Qing [Key Laboratory for Radiation Physics and Technology, Sichuan University, Chengdu 610061 (China); Luo, Guang-Nan, E-mail: gnluo@ipp.ac.cn [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031 (China); School of Nuclear Science and Technology, University of Science and Technology of China, Hefei 230026 (China)

    2014-12-15

    Experiments concerning deuterium gas-driven permeation through rolled tungsten foils in the temperature range of 850–950 K and subsequent deuterium retention have been performed. The steady state permeation flux of deuterium is proportional to the square root of the driving pressure. The permeability of deuterium is in an order of 10{sup −14} mol m{sup −1} s{sup −1} Pa{sup −1/2} in this temperature range and the activation energy for permeation is 1.21 eV. Measurements of diffusivity are significantly affected by the driving pressure, which can be well explained by a saturable-trap model. Thermal desorption spectra of samples feature a single deuterium release peak at about 873 K. TMAP 4 modeling of this peak gives a detrapping energy of 1.70 eV, which fits the dissociation enthalpy of deuterium desorbing from the inner wall of vacancy clusters or pores in tungsten.

  19. Development of Lecithin Nanoemulsion Based Organogels for Permeation Enhancement of Metoprolol through Rat Skin

    Directory of Open Access Journals (Sweden)

    J. Varshosaz

    2013-01-01

    Full Text Available Background. Drugs with low oral bioavailability due to the first pass metabolism are good candidates for transdermal delivery. Objectives. The aim of this work was preparation of transdermal nanoemulsion of metoprolol which has high first pass metabolism. Methods. Three commercially available types of lecithin (200, 100p, and 170, three short chain alcohol (n-butanol, isopropyl alcohol, and n-propanol, and isopropyl myristate (IPM were used as surfactant, cosurfactant, and oil phase, respectively. The aqueous phase was composed of metoprolol tartrate. Nanoemulsions with different surfactant/cosurfactant weight ratio, various amounts of drug, and different types of alcohol were prepared, and their phase diagrams were studied. Drug release, permeability, and diffusion coefficient of the drug were studied using hairless rat skin. Results. A significant increase in drug solution rate was observed with increasing the metoprolol content in the nanoemulsions, while it decreased when lecithin concentration increased from 40% to 60%. Increasing the water content resulted in a significant increase in metoprolol release. N-butanol enhanced the drug flux from nanoemulsions more than n-propanol and isopropyl alcohol. The o/w nanoemulsions of metoprolol showed high flux and permeability through the skin. Conclusion. Both w/o and o/w nanoemulsions of metoprolol could enhance permeation and diffusion of metoprolol through rat skin.

  20. Deuterium permeation behavior of HTUPS4 steel with thermal oxidation layer

    International Nuclear Information System (INIS)

    Xu, Yu-Ping; Liu, Feng; Zhao, Si-Xiang; Li, Xiao-Chun; Wang, Jing; An, Zhong-Qing; Lu, Tao; Liu, Hao-Dong; Ding, Fang; Zhou, Hai-Shan; Luo, Guang-Nan

    2016-01-01

    The permeation behavior of creep-resistant, Al 2 O 3 -forming HTUPS austenitic stainless steels was studied using a gas driven permeation (GDP) device. The steel samples were first thermal oxidized at air condition, followed by GDP experiments. The permeability and diffusion coefficients of oxidized samples and bare 316L steels were derived and compared. In order to characterize the oxide layer, X-ray photoelectron spectroscopy was performed. An oxide layer with a thickness of 200 nm which mainly consists of Al 2 O 3 was detected.

  1. Towards Co-evolution of Membrane Transport and Metabolism

    Science.gov (United States)

    Wei, Chenyu; Pohorille, Andrzej

    2014-01-01

    Protocellular boundaries were inextricably connected to the metabolism they encapsulated: to be inheritable, early metabolism must have led to an increased rate of growth and division of vesicles and, similarly, transport through vesicle boundaries must have supported the evolution of metabolism. Even though explaining how this coupling emerged and evolved in the absence of the complex machinery of modern cells is one of the key issues in studies on the origin of life, little is known about the biochemical and biophysical processes that might have been involved. This gap in our knowledge is a major impediment in efforts to construct scenarios for the origin of life and laboratory models of protocells. A combination of experimental and computational studies carried out by us and our collaborators is aimed at helping to close this gap. Properties of membranes might have contributed to the selection of RNA as an early biopolymer. A kinetic mechanism was proposed (Sacerdote & Szostak, 2005) in which ribose was supplied more quickly than other aldopentoses to primordial cells for preferential incorporation of ribonucleotides into nucleic acids. This proposal is based on a finding that ribose permeates membranes an order of magnitude faster than its diastereomers, arabinose and xylose. Our computer simulations, which yield permeation rates in excellent agreement with experiment, and kinetic modeling explain this phenomenon in terms of inter- and intramolecular interactions involving exocyclic hydroxyl groups attached to carbon atoms of the pyranose ring (Wei and Pohorille, 2009). They also constrain scenarios for the formation of the earliest nucleic acids (Wei and Pohorille, 2013). In one scenario, sugars permeate protocellular walls and subsequently are used to synthesize nucleic acids inside protocells. As long as this process proceeds at the rate faster than 6x10(exp -3)/s, ribose derivatives will be available for synthesis easier than their diastereomers. If

  2. Liposomal buccal mucoadhesive film for improved delivery and permeation of water-soluble vitamins.

    Science.gov (United States)

    Abd El Azim, Heba; Nafee, Noha; Ramadan, Alyaa; Khalafallah, Nawal

    2015-07-05

    This study aims at improving the buccal delivery of vitamin B6 (VB6) as a model highly water-soluble, low permeable vitamin. Two main strategies were combined; first VB6 was entrapped in liposomes, which were then formulated as mucoadhesive film. Both plain and VB6-loaded liposomes (LPs) containing Lipoid S100 and propylene glycol (∼ 200 nm) were then incorporated into mucoadhesive film composed of SCMC and HPMC. Results showed prolonged release of VB6 (72.65%, T50% diss 105 min) after 6h from LP-film compared to control film containing free VB6 (96.37%, T50% diss 30 min). Mucoadhesion was assessed both ex vivo on chicken pouch and in vivo in human. Mucoadhesive force of 0.2N and residence time of 4.4h were recorded. Ex vivo permeation of VB6, across chicken pouch mucosa indicated increased permeation from LP-systems compared to corresponding controls. Interestingly, incorporation of the vesicles in mucoadhesive film reduced the flux by 36.89% relative to LP-dispersion. Meanwhile, both films provided faster initial permeation than the liquid forms. Correlating the cumulative percent permeated ex vivo with the cumulative percent released in vitro indicated that LPs retarded VB6 release but improved permeation. These promising results represent a step forward in the field of buccal delivery of water-soluble vitamins. Copyright © 2015 Elsevier B.V. All rights reserved.

  3. Construction and performance test of apparatus for permeation experiments with controlled surfaces

    International Nuclear Information System (INIS)

    Hatano, Yuji; Nomura, Mamoru; Watanabe, Kuniaki; Livshits, Alexander I.; Busnyuk, Andrei O.; Nakamura, Yukio; Ohyabu, Nobuyoshi

    2003-01-01

    A new apparatus was constructed to examine gas-, atom- and plasma-driven permeation of hydrogen isotopes through group VA metal membranes with precisely controlled surface states. Absorption and desorption experiments are also possible. The new apparatus consists of two vacuum chambers, an upstream chamber and a downstream chamber, separated by a specimen membrane. Both chambers are evacuated by turbo-molecular pumps and sputter-ion pumps. The upstream chamber is equipped with Ta filaments serving as atomizers in atom-driven permeation experiments and cathodes in plasma-driven permeation experiments. The specimen membrane is formed into a tubular shape and electrically isolated from the chamber. Hence, ohmic heating of the membrane is possible, and this feature of the membrane is suitable for surface cleaning by high-temperature heating an impurity doping for the control of surface chemical composition through surface segregation. Both chambers were evacuated to 1 x 10 -7 Pa after baking. The main component of residual gas was H 2 , and the partial pressures of impurity gases other than H 2 were ca. 1 x 10 -8 Pa. Gas- and atom-driven permeation experiments were successfully carried out with hydrogen gas for Nb membrane activated by heating in vacuum at 1173 K. Superpermeation was observed in the atom-driven permeation experiments. Absorption experiments with a clean surface were also carried out. The surface was, however, cleaned only partially, because the temperature distribution was not uniform during high-temperature heating. Nevertheless, surface cleanliness was retained during absorption experiments under the present vacuum conditions. A new membrane assembly that will enable a uniform temperature distribution is now under construction. (author)

  4. Hydrogen permeation through metallic foils

    International Nuclear Information System (INIS)

    Bernardi, M.I.B.; Rodrigues, J.A.

    1987-01-01

    The process of electrolytic permeation of hydrogen through metallic foils is studied. A double electrolytic cell, in glass, in which the two compartments of reaction are separated by a metallic foil to be studied, was built. As direct result, the hydrogen diffusion coefficient in the metal is obtained. The hydrogen diffusion coefficients in the palladium and, in austenitic stainless steels 304 and 304 L, used in the Angra-1 reactor, were obtained. Samples of stainless steels with and without welding, were used. (Author) [pt

  5. Water vapor permeation and dehumidification performance of poly(vinyl alcohol)/lithium chloride composite membranes

    KAUST Repository

    Bui, Duc Thuan

    2015-10-09

    Thin and robust composite membranes comprising stainless steel scaffold, fine and porous TiO2 and polyvinyl alcohol/lithium chloride were fabricated and studied for air dehumidification application. Higher hydrophilicity, sorption and permeation were observed for membranes with increased lithium chloride content up to 50%. The permeation and sorption properties of the membranes were investigated under different temperatures. The results provided a deeper insight into the membrane water vapor permeation process. It was specifically noted that lithium chloride significantly reduces water diffusion energy barrier, resulting in the change of permeation energy from positive to negative values. Higher water vapor permeance was observed for the membrane with higher LiCl content at lower temperature. The isothermal air dehumidification tests show that the membrane is suitable for dehumidifying air in high humid condition. Additionally, results also indicate a trade-off between the humidity ratio drop with the water vapor removal rate when varying air flowrate.

  6. Ratio of dialytic coefficients of hydrogen and tritium in permeation through palladium alloy film

    International Nuclear Information System (INIS)

    Fujita, Haruyuki; Fujita, Kunio; Sakamoto, Hiroshi; Higashi, Kunio; Okada, Sakae.

    1982-01-01

    The dialytic coefficient for hydrogen is especially large in palladium and its alloys. Recently, with the research on fusion reactors, the dialytic coefficient of tritium permeating through solids and its isotopic effect have been the object of interest. The ratio of the dialytic coefficients of tritium and hydrogen has been usually assumed to be 3. The measurement of the dialytic coefficient in solids using pure tritium is practically difficult. Therefore, the authors carried out the experiment to determine the ratio of the dialytic coefficients of pure T 2 and pure H 2 by permeating the mixed gas of T and H through Pd-Au-Ag alloy. The mixed hydrogen gas was filled in a separation cell containing a palladium alloy tube, and the separation factor of tritium and hydrogen was measured by changing pressure, flow rate and temperature. The separation factor depends mainly on the relative dialytic coefficients of tritium and hydrogen, therefore, the ratio of dialytic coefficients can be determined by the simple analysis of the experimental results. This experimental method is suitable to determine the relative value of dialytic coefficients, and the obtained ratio was about 2.1. (Kako, I.)

  7. A novel lipid nanoemulsion system for improved permeation of granisetron.

    Science.gov (United States)

    Doh, Hea-Jeong; Jung, Yunjin; Balakrishnan, Prabagar; Cho, Hyun-Jong; Kim, Dae-Duk

    2013-01-01

    A new lipid nanoemulsion (LNE) system containing granisetron (GRN) was developed and its in vitro permeation-enhancing effect was evaluated using Caco-2 cell monolayers. Particle size, polydispersity index (PI) and stability of the prepared GRN-loaded LNE systems were also characterized. The mean diameters of prepared LNEs were around 50 nm with PI<0.2. Developed LNEs were stable at 4°C in the dark place over a period of 12 weeks. In vitro drug dissolution and cytotoxicity studies of GRN-loaded LNEs were performed. GRN-loaded LNEs exhibited significantly higher drug dissolution than GRN suspension at pH 6.8 for 2h (P<0.05). In vitro permeation study in Caco-2 cell monolayers showed that the LNEs significantly enhanced the drug permeation compared to GRN powder. The in vivo toxicity study in the rat jejunum revealed that the prepared GRN-loaded LNE was as safe as the commercial formulation (Kytril). These results suggest that LNE could be used as a potential oral liquid formulation of GRN for anti-emetic treatment on the post-operative and chemotherapeutic patients. Copyright © 2012 Elsevier B.V. All rights reserved.

  8. High permeation rates in liposome systems explain rapid glyphosate biodegradation associated with strong isotope fractionation.

    Science.gov (United States)

    Ehrl, Benno; Mogusu, Emmanuel O; Kim, Kyoungtea; Hofstetter, Heike; Pedersen, Joel A; Elsner, Martin

    2018-05-23

    Bacterial uptake of charged organic pollutants such as the widely used herbicide glyphosate is typically attributed to active transporters, whereas passive membrane permeation as an uptake pathway is usually neglected. For 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes, pH-dependent membrane permeation coefficients (Papp) of glyphosate, determined by nuclear magnetic resonance (NMR) spectroscopy, varied from Papp(pH 7.0) = 3.7 (+/-0.3) × 10-7 m∙s-1 to Papp(pH 4.1) = 4.2 (+/-0.1) × 10-6 m∙s-1. This surprisingly rapid membrane permeation depended on glyphosate speciation and was, at physiological pH, in the range of polar, non-charged molecules suggesting that passive membrane permeation is a potential uptake pathway during glyphosate biodegradation. To test this hypothesis, a Gram-negative glyphosate degrader, Ochrobactrum sp. FrEM, was isolated from glyphosate-treated soil and glyphosate permeation rates inferred from the liposome model were compared to bacterial degradation rates. Estimated maximum permeation rates were, indeed, two orders of magnitudes higher than glyphosate degradation rates. Moreover, biodegradation of millimolar glyphosate concentrations gave rise to pronounced carbon isotope fractionation with an apparent kinetic isotope effect of AKIEcarbon= 1.014 ± 0.003. This value is consistent with unmasked enzymatic isotope fractionation demonstrating that glyphosate biodegradation was little mass transfer-limited and glyphosate exchange across the cell membrane was rapid relative to enzymatic turnover.

  9. The impact of tritium solubility and diffusivity on inventory and permeation in liquid breeder blankets

    International Nuclear Information System (INIS)

    Caorlin, M.; Gervasini, G.; Reiter, F.

    1988-01-01

    The authors reviewed hydrogen solubility and diffusivity data for liquid lithium-based compounds which are potential breeding blanket materials in NET-type fusion devices. These data have been used to assess tritium permeation and inventory in separately cooled NET blankets and in self cooled blankets with a vanadium first wall. The results for the separately cooled NET-liquid breeder show that tritium permeation is negligible for lithium, a serious problem for Pb-17Li and a critical one for Flibe. The total tritium inventory is lowest in lithium, high in Pb-17Li and very high in Flibe. The high tritium partial pressure for Flibe or Pb-17Li can be reduced in a self cooled blanket with a vanadium first wall. Permeation into the plasma reduces the blanket tritium inventory and permeation. Tritium recovery can be combined with the plasma exhaust

  10. Permeation of a H2 + HD + D2 gas mixture through a polymer membrane

    International Nuclear Information System (INIS)

    Mercea, P.; Cuna, S.; Kreibik, S.; Ursu, I.

    1990-01-01

    The selective permeation of a H 2 + HD + D 2 gas mixture through a polyethylene terephthalate membrane was studied at T 20 0 C. It was found that the permeation of the HD through the membrane leads to a smaller overall hydrogen-deuterium separation factor than that determined in the permeation experiments with pure H 2 and D 2 . On the other hand, a process of isotopic exchange between deuterium atoms from the penetrant gas stream and hydrogen atoms from the polymer membrane is assumed and discussed in order to explain temporal variations of the H 2 , HD and D 2 concentrations of the permanent gas stream. (author)

  11. Feasibility of permeation grouting for constructing subsurface barriers

    International Nuclear Information System (INIS)

    Dwyer, B.P.

    1994-04-01

    Efforts are being made to devise technologies that provide interim containment of waste sites while final remediation alternatives are developed. Permeation grouting, a technique used extensively in the civil and mining engineering industry has been investigated as a method for emplacing a subsurface containment barrier beneath existing waste sites. Conceptually an underlying barrier is placed by injecting grout into the formation at less than fracturing pressure from a series of directionally drilled boreholes beneath the waste site. This study evaluated the penetration and performance characteristics in varying soil conditions of four different grout materials (two microfine cements, mineral wax, and sodium silicate) at a field scale. Field testing consisted of grout injection via sleeve (tube-a'-manchette) pipe into both vertical and horizontal borehole configurations at the Mixed Waste Landfill Integrated Demonstration site at Sandia National Laboratories. Prior to, during, and after grout injection non-intrusive geophysical techniques were used to map grout flow. Following the tests, the site was excavated to reveal details of the grout permeation, and grouted soil samples were cored for laboratory characterization. The non-intrusive and intrusive grout mapping showed preferential flow patterns, i.e., the grout tended to follow the path of least resistance. Preliminary testing indicates that permeation grouting is a feasible method for emplacing a low permeability subsurface barrier in the semi-arid unconsolidated alluvial soils common to the Southwest. Despite the success of this project, difficulties in predicting grout flow in heterogeneous soils and non-intrusive methods for imaging grout location and continuity are issues that need more attention

  12. Hydrogen permeation resistant heat pipe for bi-modal reactors. Final report, October 1, 1994--September 30, 1995

    International Nuclear Information System (INIS)

    North, M.T.; Anderson, W.G.

    1995-01-01

    The principal objective of this program was to demonstrate technology that will make a sodium heat pipe tolerant of hydrogen permeation for a bimodal space reactor application. Special focus was placed on techniques which enhance the permeation of hydrogen out of the heat pipe. Specific objectives include: define the detailed requirements for the bimodal reactor application; design and fabricate a prototype heat pipe tolerant of hydrogen permeation; and test the prototype heat pipe and demonstrate that hydrogen which permeates into the heat pipe is removed or reduced to acceptable levels. The results of the program were fully successful. Analyses were performed on two different heat pipe designs and an experimental heat pipe was fabricated and tested. A model of the experimental heat pipe was developed to predict the enhancement in the hydrogen permeation rate out of the heat pipe. A significant improvement in the rate at which hydrogen permeates out of a heat pipe was predicted for the use of the special condenser geometry developed here. Agreement between the model and the experimental results was qualitatively good. Inclusion of the additional effects of fluid flow in the heat pipe are recommended for future work

  13. Effect of piperidones on hydrogen permeation and corrosion ...

    Indian Academy of Sciences (India)

    corrosion inhibition. 3.5 Hydrogen permeation measurements. Hydrogen can enter into the metal during various industrial operations like melting, heat treatment, or pickling and electrochemical processes such as cathodic cleaning and electrolytic machining. Of the various sources of entry of hydrogen into the metal,.

  14. In vitro permeation of palladium powders through intact and damaged human skin.

    Science.gov (United States)

    Crosera, Matteo; Mauro, Marcella; Bovenzi, Massimo; Adami, Gianpiero; Baracchini, Elena; Maina, Giovanni; Larese Filon, Francesca

    2018-05-01

    The use of palladium (Pd) has grown in the last decades, commonly used in automotive catalytic converters, jewellery and dental restorations sectors. Both general and working population can be exposed to this metal, which may act as skin sensitizer. This study investigated in vitro palladium powders permeation through excised intact and damaged human skin using the Franz diffusion cell method and the effect of rapid skin decontamination using sodium laureth-sulphate. 1 mL of a 10 min sonicated suspension made of 2.5 g of Pd powder in 50 mL synthetic sweat at pH 4.5 and room temperature was applied to the outer surface of the skin membranes for 24 h. Pd permeation, assessed by ICP-MS, was higher when damaged skin was used (p = 0.03). Final flux permeation values and lag times were 0.02 ± 0.01 μg cm -2  h -1 and 6.00 ± 3.95 h for intact, and 0.10 ± 0.02 μg cm -2  h -1 and 2.05 ± 1.49 h for damaged skin samples, respectively. Damaged skin protocol enhances Pd skin penetration inside dermal layer (p = 0.04), thus making the metal available for systemic uptake. Pd penetration (p = 0.02) and permeation (p = 0.012) through intact skin decreased significantly when a cleaning procedure was applied. This study demonstrates that after skin exposure to Pd powders a small permeation of the metal happen both through intact and damaged skin and that an early decontamination with a common cleanser can significantly decrease the final amount of metal available forsystemic uptake. Copyright © 2018 Elsevier B.V. All rights reserved.

  15. Analysis of transient permeation behavior of hydrogen isotope caused by abrupt temperature change of first wall and blanket wall material

    International Nuclear Information System (INIS)

    Yamawaki, Michio; Tanaka, Satoru; Kiyoshi, Tsukasa

    1989-01-01

    To obtain further information on the transient permeation behavior of hydrogen isotopes as caused by an abrupt temperature change, numerical calculations were carried out for two typical metals, nickel and vanadium. Deuterium permeation through nickel is analyzed as a typical case of bulk-diffusion-limited permeation. Its transient behavior changed dramatically according to the specimen thickness. The transient behavior, in general, is separated into two parts, initial and latter period behaviors. Conditions which cause such a separation were evaluated. Evaluation of the hydrogen diffusivity and solubility by an analysis of transient curves of hydrogen permeation was carried out. The transient behavior of simultaneous gas- and ion-driven hydrogen permeation through vanadium was also analyzed. Overshooting of the hydrogen permeation rate appears with an abrupt temperature increase. Increasing the impinging ion flux causes the overshooting peak to become sharper, and also reduces the change of the steady-state permeation rate to be attained after the temperature change compared with the initial value. (orig.)

  16. Ozonation of nanofiltration permeate of whey before processing by reverse osmosis

    Directory of Open Access Journals (Sweden)

    Zmievskii Yurii G.

    2017-01-01

    Full Text Available During nanofiltration processing of whey a significant amount of permeate is generated. In some cases this permeate is treated by reverse osmosis to get purified water for technological needs. Dry substances are not used, because they contain practically the same amount of organic and inorganic components. Mineral substances can be used for the mineralization of drinking water purified by reverse osmosis. However, the presence of organic compounds complicates the process of separation, as well as reduces the specific productivity of reverse osmosis membranes at the concentration stage. Therefore, the search for methods of destruction and removal of organic components is grounded. In the presented work, experimental studies of ozonation and sorption of organic compounds by activated carbon were carried. It has been shown that ozonation improves the degree of sorption purification by six times. Sequential treatment with ozone and subsequent filtration through the layer of activated carbon improves the specific productivity of reverse osmosis membranes by 30% at the stage of treatment of the nanofiltration permeate, while their selectivity remains unchanged.

  17. Bias-Exchange Metadynamics Simulation of Membrane Permeation of 20 Amino Acids.

    Science.gov (United States)

    Cao, Zanxia; Bian, Yunqiang; Hu, Guodong; Zhao, Liling; Kong, Zhenzhen; Yang, Yuedong; Wang, Jihua; Zhou, Yaoqi

    2018-03-16

    Thermodynamics of the permeation of amino acids from water to lipid bilayers is an important first step for understanding the mechanism of cell-permeating peptides and the thermodynamics of membrane protein structure and stability. In this work, we employed bias-exchange metadynamics simulations to simulate the membrane permeation of all 20 amino acids from water to the center of a dipalmitoylphosphatidylcholine (DPPC) membrane (consists of 256 lipids) by using both directional and torsion angles for conformational sampling. The overall accuracy for the free energy profiles obtained is supported by significant correlation coefficients (correlation coefficient at 0.5-0.6) between our results and previous experimental or computational studies. The free energy profiles indicated that (1) polar amino acids have larger free energy barriers than nonpolar amino acids; (2) negatively charged amino acids are the most difficult to enter into the membrane; and (3) conformational transitions for many amino acids during membrane crossing is the key for reduced free energy barriers. These results represent the first set of simulated free energy profiles of membrane crossing for all 20 amino acids.

  18. Bias-Exchange Metadynamics Simulation of Membrane Permeation of 20 Amino Acids

    Directory of Open Access Journals (Sweden)

    Zanxia Cao

    2018-03-01

    Full Text Available Thermodynamics of the permeation of amino acids from water to lipid bilayers is an important first step for understanding the mechanism of cell-permeating peptides and the thermodynamics of membrane protein structure and stability. In this work, we employed bias-exchange metadynamics simulations to simulate the membrane permeation of all 20 amino acids from water to the center of a dipalmitoylphosphatidylcholine (DPPC membrane (consists of 256 lipids by using both directional and torsion angles for conformational sampling. The overall accuracy for the free energy profiles obtained is supported by significant correlation coefficients (correlation coefficient at 0.5–0.6 between our results and previous experimental or computational studies. The free energy profiles indicated that (1 polar amino acids have larger free energy barriers than nonpolar amino acids; (2 negatively charged amino acids are the most difficult to enter into the membrane; and (3 conformational transitions for many amino acids during membrane crossing is the key for reduced free energy barriers. These results represent the first set of simulated free energy profiles of membrane crossing for all 20 amino acids.

  19. What is Metabolic Syndrome?

    Science.gov (United States)

    ... Intramural Research Home / Metabolic Syndrome Metabolic Syndrome Also known as What Is Metabolic syndrome ... metabolic risk factors to be diagnosed with metabolic syndrome. Metabolic Risk Factors A Large Waistline Having a large ...

  20. Tritium permeation and recovery

    International Nuclear Information System (INIS)

    Bond, R.A.; Hamilton, A.M.

    1987-01-01

    The paper is an appendix to a study of the reactor relevance of the NET design concept. The latter study examines whether the technologies and design principles proposed for NET can be directly extrapolated to a demonstration (DEMO) reactor. In this appendix, tritium transport in the DEMO breeding blanket is considered with emphasis on the permeation rate from the lithium-lead breeder into the coolant. A computational model used to calculate the tritium transport in the breeder blanket is described. Results are reported for the tritium transport in the NET/INTOR type blanket as well as the DEMO blanket in order to provide a comparison. In addition, results are presented for the helium coolant tritium extraction analysis. (U.K.)

  1. Effect of skim milk treated with high hydrostatic pressure on permeate flux and fouling during ultrafiltration.

    Science.gov (United States)

    Leu, Mathilde; Marciniak, Alice; Chamberland, Julien; Pouliot, Yves; Bazinet, Laurent; Doyen, Alain

    2017-09-01

    Ultrafiltration (UF) is largely used in the dairy industry to generate milk and whey protein concentrate for standardization of milk or production of dairy ingredients. Recently, it was demonstrated that high hydrostatic pressure (HHP) extended the shelf life of milk and improved rennet coagulation and cheese yield. Pressurization also modified casein micelle size distribution and promoted aggregation of whey proteins. These changes are likely to affect UF performance. Consequently, this study determined the effect of skim milk pressurization (300 and 600 MPa, 5 min) on UF performance in terms of permeate flux decline and fouling. The effect of HHP on milk proteins was first studied and UF was performed in total recycle mode at different transmembrane pressures to determine optimal UF operational parameters and to evaluate the effect of pressurization on critical and limiting fluxes. Ultrafiltration was also performed in concentration mode at a transmembrane pressure of 345 kPa for 130 or 140 min to evaluate the decline of permeate flux and to determine fouling resistances. It was observed that average casein micelle size decreased by 32 and 38%, whereas β-lactoglobulin denaturation reached 30 and 70% at 300 and 600 MPa, respectively. These results were directly related to UF performance because initial permeate fluxes in total recycle mode decreased by 25% at 300 and 600 MPa compared with nonpressurized milk, critical flux, and limiting flux, which were lower during UF of milk treated with HHP. During UF in concentration mode, initial permeate fluxes were 30% lower at 300 and 600 MPa compared with the control, but the total flux decline was higher for nonpressurized milk (62%) compared with pressure-treated milk (30%). Fouling resistances were similar, whatever the treatment, except at 600 MPa where irreversible fouling was higher. Characterization of the fouling layer showed that caseins and β-lactoglobulin were mainly involved in membrane fouling after UF of

  2. Experimental measurements of the permeation of hydrogen isotopes in lithium filled niobium cells

    International Nuclear Information System (INIS)

    Goodall, D.H.J.; McCracken, G.M.; Austin, G.E.

    1976-01-01

    Lithium filled niobium cells have been heated in vacua at temperatures in the range 300 to 900 0 C. By measuring the flow of deuterium into the cell it has been possible to make estimates of the rate of permeation of deuterium in the niobium wall. After initial fast diffusion into the capsule the rate of permeation becomes very much slower than that determined by diffusion in the bulk niobium indicating that a second, slower, rate process is involved. Measurements of the rate of deuterium permeation out of the cell have been made for a number of different cell geometries and a range of temperatures. The results indicate that the slow rate process, which is dominant at low concentrations, is the desorption step from the metal to the gas phase

  3. Comparing pervaporation and vapor permeation hybrid distillation processes

    NARCIS (Netherlands)

    Fontalvo, J.; Cuellar, P.; Timmer, J.M.K.; Vorstman, M.A.G.; Wijers, J.G.; Keurentjes, J.T.F.

    2005-01-01

    Previous studies have shown that hybrid distillation processes using either pervaporation or vapor permeation can be very attractive for the separation of mixtures. In this paper, a comparison between these two hybrid processes has been made. A tool has been presented that can assist designers and

  4. 40 CFR 1051.515 - How do I test my fuel tank for permeation emissions?

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 32 2010-07-01 2010-07-01 false How do I test my fuel tank for... Procedures § 1051.515 How do I test my fuel tank for permeation emissions? Measure permeation emissions by weighing a sealed fuel tank before and after a temperature-controlled soak. (a) Preconditioning fuel soak...

  5. Fully automatic flow-based device for monitoring of drug permeation across a cell monolayer.

    Science.gov (United States)

    Zelená, Lucie; Marques, Sara S; Segundo, Marcela A; Miró, Manuel; Pávek, Petr; Sklenářová, Hana; Solich, Petr

    2016-01-01

    A novel flow-programming setup based on the sequential injection principle is herein proposed for on-line monitoring of temporal events in cell permeation studies. The permeation unit consists of a Franz cell with its basolateral compartment mixed under mechanical agitation and thermostated at 37 °C. The apical compartment is replaced by commercially available Transwell inserts with a precultivated cell monolayer. The transport of drug substances across epithelial cells genetically modified with the P-glycoprotein membrane transporter (MDCKII-MDR1) is monitored on-line using rhodamine 123 as a fluorescent marker. The permeation kinetics of the marker is obtained in a fully automated mode by sampling minute volumes of solution from the basolateral compartment in short intervals (10 min) up to 4 h. The effect of a P-glycoprotein transporter inhibitor, verapamil as a model drug, on the efficiency of the marker transport across the cell monolayer is thoroughly investigated. The analytical features of the proposed flow method for cell permeation studies in real time are critically compared against conventional batch-wise procedures and microfluidic devices.

  6. Impact of Humidity on In Vitro Human Skin Permeation Experiments for Predicting In Vivo Permeability.

    Science.gov (United States)

    Ishida, Masahiro; Takeuchi, Hiroyuki; Endo, Hiromi; Yamaguchi, Jun-Ichi

    2015-12-01

    In vitro skin permeation studies have been commonly conducted to predict in vivo permeability for the development of transdermal therapeutic systems (TTSs). We clarified the impact of humidity on in vitro human skin permeation of two TTSs having different breathability and then elucidated the predictability of in vivo permeability based on in vitro experimental data. Nicotinell(®) TTS(®) 20 and Frandol(®) tape 40mg were used as model TTSs in this study. The in vitro human skin permeation experiments were conducted under humidity levels similar to those used in clinical trials (approximately 50%) as well as under higher humidity levels (approximately 95%). The skin permeability values of drugs at 95% humidity were higher than those at 50% humidity. The time profiles of the human plasma concentrations after TTS application fitted well with the clinical data when predicted based on the in vitro permeation parameters at 50% humidity. On the other hand, those profiles predicted based on the parameters at 95% humidity were overestimated. The impact of humidity was higher for the more breathable TTS; Frandol(®) tape 40mg. These results show that in vitro human skin permeation experiments should be investigated under realistic clinical humidity levels especially for breathable TTSs. © 2015 Wiley Periodicals, Inc. and the American Pharmacists Association.

  7. d-Tagatose production by permeabilized and immobilized Lactobacillus plantarum using whey permeate.

    Science.gov (United States)

    Jayamuthunagai, J; Srisowmeya, G; Chakravarthy, M; Gautam, P

    2017-07-01

    The aim of the work is to produce d-Tagatose by direct addition of alginate immobilized Lactobacillus plantarum cells to lactose hydrolysed whey permeate. The cells were untreated and immobilized (UIC), permeabilized and immobilized (PIC) and the relative activities were compared with purified l-arabinose isomerase (l-AI) for d-galactose isomerization. Successive lactose hydrolysis by β-galactosidase from Escherichia coli and d-galactose isomerization using l-AI from Lactobacillus plantarum was performed to investigate the in vivo production of d-tagatose in whey permeate. In whey permeate, maximum conversion of 38% and 33% (w/w) d-galactose isomerization by PIC and UIC has been obtained. 162mg/g and 141mg/g of d-tagatose production was recorded in a 48h reaction time at 50°C, pH 7.0 with 5mM Mn 2+ ion concentration in the initial substrate mixture. Copyright © 2017 Elsevier Ltd. All rights reserved.

  8. Transdermal delivery of diclofenac using water-in-oil microemulsion: formulation and mechanistic approach of drug skin permeation.

    Science.gov (United States)

    Thakkar, Priyanka J; Madan, Parshotam; Lin, Senshang

    2014-05-01

    The objective of the present investigation was to enhance skin permeation of diclofenac using water-in-oil microemulsion and to elucidate its skin permeation mechanism. The w/o microemulsion formulations were selected based on constructed pseudoternary phase diagrams depending on water solubilization capacity and thermodynamic stability. These formulations were also subjected to physical characterization based on droplet size, viscosity, pH and conductivity. Permeation of diclofenac across rat skin using side-by-side permeation cells from selected w/o microemulsion formulations were evaluated and compared with control formulations. The selected w/o microemulsion formulations were thermodynamically stable, and incorporation of diclofenac sodium into microemulsion did not affect the phase behavior of system. All microemulsion formulations had very low viscosity (11-17 cps) and droplet size range of 30-160 nm. Microemulsion formulations exhibited statistically significant increase in diclofenac permeation compared to oily solution, aqueous solution and oil-Smix solution. Higher skin permeation of diclofenac was observed with low Smix concentration and smaller droplet size. Increase in diclofenac loading in aqueous phase decreased the partition of diclofenac. Diclofenac from the oil phase of microemulsion could directly partition into skin, while diclofenac from the aqueous droplets was carried through skin by carrier effect.

  9. Permeation of antigen protein-conjugated nanoparticles and live bacteria through microneedle-treated mouse skin

    Directory of Open Access Journals (Sweden)

    Kumar A

    2011-06-01

    Full Text Available Amit Kumar, Xinran Li, Michael A Sandoval, B Leticia Rodriguez, Brian R Sloat, Zhengrong CuiUniversity of Texas at Austin, College of Pharmacy, Pharmaceutics Division, Austin, TX, USABackground: The present study was designed to evaluate the extent to which pretreatment with microneedles can enhance skin permeation of nanoparticles in vitro and in vivo. Permeation of live bacteria, which are physically nanoparticles or microparticles, through mouse skin pretreated with microneedles was also studied to evaluate the potential risk of microbial infection.Methods and results: It was found that pretreatment of mouse skin with microneedles allowed permeation of solid lipid nanoparticles, size 230 nm, with ovalbumin conjugated on their surface. Transcutaneous immunization in a mouse skin area pretreated with microneedles with ovalbumin nanoparticles induced a stronger antiovalbumin antibody response than using ovalbumin alone. The dose of ovalbumin antigen determined whether microneedle-mediated transcutaneous immunization with ovalbumin nanoparticles induced a stronger immune response than subcutaneous injection of the same ovalbumin nanoparticles. Microneedle treatment permitted skin permeation of live Escherichia coli, but the extent of the permeation was not greater than that enabled by hypodermic injection.Conclusion: Transcutaneous immunization on a microneedle-treated skin area with antigens carried by nanoparticles can potentially induce a strong immune response, and the risk of bacterial infection associated with microneedle treatment is no greater than that with a hypodermic injection.Keywords: antibody responses, safety of microneedles, transepidermal water loss

  10. Effect of Microneedle Type on Transdermal Permeation of Rizatriptan.

    Science.gov (United States)

    Uppuluri, Chandrateja; Shaik, Ashraf Sultana; Han, Tao; Nayak, Atul; Nair, Karthik J; Whiteside, Benjamin R; Nalluri, Buchi N; Das, Diganta B

    2017-07-01

    The present study was aimed to investigate the effect of salient microneedle (MN) geometry parameters like length, density, shape and type on transdermal permeation of rizatriptan (RIZ). Studies were carried out using two types of MN devices viz. AdminPatch® arrays (ADM) (0.6, 0.9, 1.2 and 1.5 mm lengths) and laboratory-fabricated polymeric MNs (PMs) of 0.6 mm length. In the case of the PMs, arrays were applied three times at different places within a 1.77-cm 2 skin area (PM-3) to maintain the MN density closer to 0.6 mm ADM. Histological studies revealed that PM, owing to their geometry/design, formed wider and deeper microconduits when compared to ADM of similar length. Approximately 4.9- and 4.2-fold increases in the RIZ steady-state flux values were observed with 1.5 mm ADM and PM-3 applications when compared to the passive studies. A good correlation between different dimensionless parameters like the amount of RIZ permeated (C t /C s ), thickness (h/L) and surface area (S a /L 2 ) of the skin was observed with scaling analyses. Numerical simulations provided further information regarding the distribution of RIZ in MN-treated skin after application of different MNs. Overall, the study suggests that MN application enhances the RIZ transdermal permeation and the geometrical parameters of MNs play an important role in the degree enhancement.

  11. Study on low temperature plasma driven permeation of hydrogen

    Energy Technology Data Exchange (ETDEWEB)

    Takizawa, Masayuki [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

    1998-03-01

    It is one of the most important problem in PWI of fusion devices from the point of view of tritium leakage that hydrogen diffuses in the wall of the device and permeates through it, which results in hydrogen being released to the coolant side. In this study, plasma driven permeation experiments were carried out with several kinds of metal membranes in the low temperature plasma where ionic and atomic hydrogen as well as electron existed in order to survey PDP mechanism from the many view points. In addition, incident flux rate from the plasma to the membrane surface was evaluated by calculation analysis. As a result the mechanism of low temperature PDP was found out and described as PDP models. The simulation of the membrane pump system was executed and the system performance was estimated with the models. (author). 135 refs.

  12. Study on low temperature plasma driven permeation of hydrogen

    International Nuclear Information System (INIS)

    Takizawa, Masayuki

    1998-03-01

    It is one of the most important problem in PWI of fusion devices from the point of view of tritium leakage that hydrogen diffuses in the wall of the device and permeates through it, which results in hydrogen being released to the coolant side. In this study, plasma driven permeation experiments were carried out with several kinds of metal membranes in the low temperature plasma where ionic and atomic hydrogen as well as electron existed in order to survey PDP mechanism from the many view points. In addition, incident flux rate from the plasma to the membrane surface was evaluated by calculation analysis. As a result the mechanism of low temperature PDP was found out and described as PDP models. The simulation of the membrane pump system was executed and the system performance was estimated with the models. (author). 135 refs

  13. Investigation of non-magnetic alloys for the suppression of tritium permeation. Final report

    International Nuclear Information System (INIS)

    1980-07-01

    This report describes a small (300 man hour) literature survey relating to the suppression of tritium loss by permeation through the walls of fusion reactors. The program was based on prior in-house Thermacore work to suppress hydrogen permeation into high temperature (800 0 C) heat pipes. The Thermacore approach involves selection of a steel with a small (.5 to 5%) aluminum content. The aluminum is diffused to the surface and oxidized. The present work was aimed at identification of alloys which might combine low tritium permeation with other properties desired in fusion reactor vessels, heat exchangers, lithium-handling plumbing and other components likely to contain tritium. These properties include low radiation damage, low magnetic permeability, high temperature strength, and compatibility with potential heat transfer and blanket materials. The work consisted of two tasks: Problem Definition and Literature Search and Analysis

  14. Physics-Based Modeling of Permeation: Simulation of Low-Volatility Agent Permeation and Aerosol Vapor Liquid Assessment Group Experiments

    Science.gov (United States)

    2015-06-01

    methylphosphonothiolate (VX) through natural latex rubber and neoprene resulting from LVAP tests. 2. The permeation model is used to study the sensitivity of...Styrene–Butadiene– Rubber , Ethylene–Propylene–Diene Terpolymer, and Natural Rubber Versus Hydrocarbons (C8–C16). Macromolecules 1991, 24 (9), 2598–2605...22 14. Harogoppad, S.B.; Aminabhavi, T.M. Diffusion and Sorption of Organic Liquids through Polymer Membranes 2. Neoprene, SBR, EPDM, NBR , and

  15. The ‘ideal selectivity’ vs ‘true selectivity’ for permeation of gas mixture in nanoporous membranes

    Science.gov (United States)

    He, Zhou; Wang, Kean

    2018-03-01

    In this study, we proposed and validated a novel and non-destructive experimental technology for measuring the permeation of binary gas mixture in nanoporous membranes. The traditional time lag rig was modified to examine the permeation characteristics of each gas component as well as that of the binary gas mixtures. The difference in boiling points of each species were explored. Binary gas mixtures of CO2/He were permeated through the nanoporous carbon molecular sieve membrane (CMSM). The results showed that, due to the strong interaction among different molecules and with the porous network of the membrane, the measured perm-selectivity or ‘true selectivity’ of a binary mixture can significantly deviate from the ‘ideal selectivity’ calculated form the permeation flux of each pure species, and this deviation is a complicated function of the molecular properties and operation conditions.

  16. Phase Change Permeation Technology for Environmental Control & Life Support Systems

    Data.gov (United States)

    National Aeronautics and Space Administration — NASA is evaluating Dutyion™, a phase change permeation membrane technology developed by Design Technology and Irrigation (DTI), for use in future advanced life...

  17. PermeaLoop™, a novel in vitro tool for small-scale drug-dissolution/permeation studies

    DEFF Research Database (Denmark)

    Sironi, Daniel; Rosenberg, Jörg; Bauer-Brandl, Annette

    2018-01-01

    dissolution and permeation, as it is occurring in vivo. We propose a novel setup with a high area-to-volume ratio and report as a model case the dissolution/permeation behavior of an enabling formulation of the poorly soluble and poorly permeable drug ABT-869. Mini tablets consisting of an amorphous solid......Loop™ is regarded a promising tool for evaluating enabling formulations....

  18. Investigation on the suitability of plasma sprayed Fe-Cr-Al coatings as tritium permeation barrier

    International Nuclear Information System (INIS)

    Fazio, C.; Serra, E.; Benamati, G.

    1999-01-01

    Results on the fabrication of a tritium permeation barrier by spraying Fe-Cr-Al powders are described. The sprayed coatings were deposited at temperatures below the A c1 temperature of the ferritic-martensitic steel substrate and no post-deposition heat treatment was applied. The aim of the investigation was the determination of the efficiency of the coatings to act as tritium permeation barrier. Metallurgical investigations as well as hydrogen isotope permeation measurements were carried out onto the produced coatings. The depositions were performed on ferritic-martensitic steels by means of three types of spray techniques: high velocity oxy fuel, air plasma spray and vacuum plasma spray. (orig.)

  19. Liquid and Gas Permeation Studies on the Structure and Properties of Polyamide Thin-Film Composite Membranes

    KAUST Repository

    Duan, Jintang

    2014-01-01

    layer by gas adsorption and gas permeation measurements. Gas adsorption isotherms (N2 at 77K, CO2 at 273K) confirmed the microporous nature of PA in comparison with dense CTA and polysulfone materials. Gas permeation through the commercial PA

  20. Implanted-tritium permeation experiments

    International Nuclear Information System (INIS)

    Longhurst, G.R.; Holland, D.F.; Casper, L.A.; Hsu, P.Y.; Miller, L.G.; Schmunk, R.E.; Watts, K.D.; Wilson, C.J.; Kershner, C.J.; Rogers, M.L.

    1982-04-01

    In fusion reactors, charge exchange neutral atoms of tritium coming from the plasma will be implanted into the first wall and other interior structures. EG and G Idaho is conducting two experiments to determine the magnitude of permeation into the coolant streams and the retention of tritium in those structures. One experiment uses an ion gun to implant deuterium. The ion gun will permit measurements to be made for a variety of implantation energies and fluxes. The second experiment utilizes a fission reactor to generate a tritium implantation flux by the 3 He(n,p) 3 H reaction. This experiment will simulate the fusion reactor radiation environment. We also plan to verify a supporting analytical code development program, in progress, by these experiments

  1. Hydrogen permeation in FeCrAl alloys for LWR cladding application

    Science.gov (United States)

    Hu, Xunxiang; Terrani, Kurt A.; Wirth, Brian D.; Snead, Lance L.

    2015-06-01

    FeCrAl, an advanced oxidation-resistant iron-based alloy class, is a highly prevalent candidate as an accident-tolerant fuel cladding material. Compared with traditional zirconium alloy fuel cladding, increased tritium permeation through FeCrAl fuel cladding to the primary coolant is expected, raising potential safety concerns. In this study, the hydrogen permeability of several FeCrAl alloys was obtained using a static permeation test station, which was calibrated and validated using 304 stainless steel. The high hydrogen permeability of FeCrAl alloys leads to concerns with respect to potentially significant tritium release when used for fuel cladding in LWRs. The total tritium inventory inside the primary coolant of a light water reactor was quantified by applying a 1-dimensional steady state tritium diffusion model to demonstrate the dependence of tritium inventory on fuel cladding type. Furthermore, potential mitigation strategies for tritium release from FeCrAl fuel cladding were discussed and indicate the potential for application of an alumina layer on the inner clad surface to serve as a tritium barrier. More effort is required to develop a robust, economical mitigation strategy for tritium permeation in reactors using FeCrAl clad fuel assemblies.

  2. Dynamic permeation sources for volatile organic compounds (VOCS): 'a standards test environment' nuclear track detector

    International Nuclear Information System (INIS)

    Hussain, A.; Marr, I.

    2000-01-01

    The generation of a test environment for trace VOCs in urban air or work place has never been easy. This investigation is concerned with the loss rates of VOCs through polythene membrane of different thickness. Permeation glass sample bottles were suspended in the chamber with water jacket at 24 deg. C -+ 0.5 deg. temperature. The condenser was connected with a stream of nitrogen gas at a flow rate of 75-ml min/sup -1 and further diluted with air 500-ml min/sup -1/. The loss in weight of VOCs in each bottle was determined regularly, every 24 hours, with a good agreement. The loss rate depends upon temperature of the bath, thickness of the polythene, internal diameter of the permeation bottle opening. However the loss rate from permeation tubes also depends upon the solubility of the VOCs in the polymer. It is generally believed that the vapors of VOCs in the permeation bottle are dissolved in the polythene sheet (making some sort of solution) and are eventually evaporated out of it. It was observed that the loss rate per minute for benzene > toluene. This simple technique described 'generation of test environment through dynamic permeation source' could be suitable for preparing mixture of benzene, toluene and xylene in atmosphere at ppm levels or lower, with good stability, reliability and also for other compounds of atmospheric interest. (author)

  3. Permeation of Mixed Penetrants through Glassy Polymer Membranes.

    Science.gov (United States)

    1985-03-15

    and LOPE. Also, ESCA was used in conjunction with plasma etching to determine the effects of the gas phase fluorine concentration and fluorination...at 35 3C. ARD-AISS5 65 PERMEATION OF MIXED PENETRANTS THROUGH GLASSY POLYMER 213 MENBRANES (U) NORTH CAROLINA STATE UNIV AT RALEIGH R T CHERN ET AL. 15

  4. Investigation on hydrogen permeation on heat exchanger materials in conditions of steam coal gasification

    International Nuclear Information System (INIS)

    Moellenhoff, H.

    1984-01-01

    The permeation of hydrogen through iron-based alloys of different compositions in the temperature range between 700 and 1000 0 C was examined in a laboratory fluidized bed in the conditions of steam/coal gasification. Apart from tests on bright metal samples, measurement in the gasification atmosphere at a maximum pressure of 1 bar were carried out during oxidation of the metal. Experiments in a steam/hydrogen/argon mixture with the same oxidation potential were used for comparison purposes. The hydrogen permeated through the metal sample was taken to a gas chromatograph with argon flushing gas and analyzed there. The investigations on bright steel samples of various composition showed that their permeabilities for hydrogen at temperatures around 900 0 C only differed by a maximum of ± 30%. Effective prevention of permeation is therefore not possible simply by choosing a suitable alloy. If the steels are oxidized during permeation measurements, there is a reduction of the hydrogen permeability by 2 or 3 orders of magnitude due to the oxidation process, both in the steam/coal gasification fluidized bed and in a pure steam/hydrogen/argon mixture. (orig./GG) [de

  5. Development of Acyclovir-Loaded Albumin Nanoparticles and Improvement of Acyclovir Permeation Across Human Corneal Epithelial T Cells.

    Science.gov (United States)

    Suwannoi, Panita; Chomnawang, Mullika; Sarisuta, Narong; Reichl, Stephan; Müller-Goymann, Christel C

    2017-12-01

    The aim of the present study was to develop acyclovir (ACV) ocular drug delivery systems of bovine serum albumin (BSA) nanoparticles as well as to assess their in vitro transcorneal permeation across human corneal epithelial (HCE-T) cell multilayers. The ACV-loaded BSA nanoparticles were prepared by desolvation method along with physicochemical characterization, cytotoxicity, as well as in vitro transcorneal permeation studies across HCE-T cell multilayers. The nanoparticles appeared to be spherical in shape and nearly uniform in size of about 200 nm. The size of nanoparticles became smaller with decreasing BSA concentration, while the ratios of water to ethanol seemed not to affect the size. Increasing the amount of ethanol in desolvation process led to significant reduction of drug entrapment of nanoparticles with smaller size and more uniformity. The ACV-loaded BSA nanoparticles prepared were shown to have no cytotoxic effect on HCE-T cells used in permeation studies. The in vitro transcorneal permeation results revealed that ACV could permeate through the HCE-T cell multilayers significantly higher from BSA nanoparticles than from aqueous ACV solutions. The ACV-loaded BSA nanoparticles could be prepared by desolvation method without glutaraldehyde in the formulation. ACV could increasingly permeate through the multilayers of HCE-T cells from the ACV-loaded BSA nanoparticles. Therefore, the ACV-loaded BSA nanoparticles could be a highly potential ocular drug delivery system.

  6. Permeation of hair dye ingredients, p-phenylenediamine and aminophenol isomers, through protective gloves.

    Science.gov (United States)

    Lee, Hsiao-Shu; Lin, Yu-Wen

    2009-04-01

    Skin irritation and contact allergies are skin disorders common to hairdressers. The predominant oxidative hair dye components, such as p-phenylenediamine (PPD) and aminophenol isomers, can cause contact dermatitis. Use of protective gloves can prevent dermal contact with skin irritants. This study investigates the permeation behaviors of p-aminophenol (PAP), m-aminophenol (MAP), o-aminophenol (OAP) and PPD in single and mixed challenge solutions with disposable natural rubber latex (NRL) gloves, disposable polyvinylchloride (PVC) gloves and neoprene (NP) gloves. The challenge solutions were 4% PPD (w/v), 3% OAP (w/v), 2% PAP (w/v) and 2% MAP (w/v) in ethanol or 12% hydrogen peroxide solutions. The cocktail solutions of the four chemicals were also tested. An American Society for Testing and Materials type permeation cell, ethanol liquid collection and gas chromatography-flame ionization detection of samples taken from the collection medium every 10 min facilitated determination of breakthrough times (BTs), cumulative permeated masses and steady-state permeation rates (SSPRs). Experiments were 4 h long for the NRL and PVC gloves and 8 h for NP gloves. No chemicals tested broke through the NP gloves when exposed for 8 h. In the ethanol solution, PPD and OAP started breaking through the PVC gloves at 40 min. The SSPRs of PVC gloves were higher than those for NRL gloves in all challenge conditions for both single chemicals and mixtures. No tested chemicals in hydrogen peroxide solutions permeated the gloves during the 4-h tests. The chemical composition of the challenge solution was a main effecter of BTs and SSPRs for the NRL glove. For disposable PVC gloves, the main factors of BTs were molecular size [molar volume (MV)] and polarity (logK(ow)), and the primary factors of SSPRs were concentration, MV and logK(ow). In conclusion, disposable NRL gloves and disposable PVC gloves should not be used repeatedly for handling the hair dye products. Hydrogen peroxide did not

  7. Permeation mechanisms of pulsed microwave plasma deposited silicon oxide films for food packaging applications

    International Nuclear Information System (INIS)

    Deilmann, Michael; Grabowski, Mirko; Theiss, Sebastian; Bibinov, Nikita; Awakowicz, Peter

    2008-01-01

    Silicon oxide barrier layers are deposited on polyethylene terephthalate as permeation barriers for food packaging applications by means of a low pressure microwave plasma. Hexamethyldisiloxane (HMDSO) and oxygen are used as process gases to deposit SiO x coatings via pulsed low pressure plasmas. The layer composition of the coating is investigated by Fourier transform infrared spectroscopy and energy dispersive x-ray spectroscopy to show correlations with barrier properties of the films. The oxygen permeation barrier is determined by the carrier gas method using an electrochemical detector. The transition from low to high barrier films is mapped by the transition from organic SiO x C y H z layers to quartz-like SiO 1.7 films containing silanol bound hydrogen. A residual permeation as low as J = 1 ± 0.3 cm 3 m -2 day -1 bar -1 is achieved, which is a good value for food packaging applications. Additionally, the activation energy E p of oxygen permeation is analysed and a strong increase from E p = 31.5 kJ mol -1 for SiO x C y H z -like coatings to E p = 53.7 kJ mol -1 for SiO 1.7 films is observed by increasing the oxygen dilution of HMDSO:O 2 plasma. The reason for the residual permeation of high barrier films is discussed and coating defects are visualized by capacitively coupled atomic oxygen plasma etching of coated substrates. A defect density of 3000 mm -2 is revealed

  8. Effects of thin films on inventory, permeation and re-emission of energetic hydrogen

    International Nuclear Information System (INIS)

    Ohyabu, N.; Nakamura, Y.; Nakahara, Y.; Livshits, A.; Alimov, V.; Busnyuk, A.; Notkin, M.; Samartsev, A.; Doroshin, A.

    2000-01-01

    A non-metallic coating thicker than the implantation depth may protect a metal against tritium retention and permeation. However, a thinner film has quite the opposite effect: a dramatic increase of permeation and retention, and a corresponding suppression of re-emission. In view of the benefits expected from particle control with a superpermeable membrane placed right inside the divertor, the behavior of a Nb sample was investigated in a plasma-membrane device having a graphite target. Even polyatomic carbide coating was found not to hinder hydrogen absorption and permeation. Polyatomic non-carbide C films effectively inhibits it, but the formation of such films depends on H and C fluxes, H energy and metal temperature. A durable isolation of suprathermal hydrogen with the superpermeable membrane was observed at a high enough ratio between H and C fluxes, and the effects of carbon were found to have a non-monotonic temperature dependence

  9. GAS PERMEATION PROPERTIES OF POLY(LACTIC ACID). (R826733)

    Science.gov (United States)

    AbstractThe need for the development of polymeric materials based on renewable resources has led to the development of poly(lactic acid) (PLA) which is being produced from a feedstock of corn rather than petroleum. The present study examines the permeation of nitrogen...

  10. Hydrogen permeation measurement of the reduced activation ferritic steel F82H by the vacuum thermo-balance method

    International Nuclear Information System (INIS)

    Yoshida, Hajime; Enoeda, Mikio; Abe, Tetsuya; Akiba, Masato

    2005-03-01

    Hydrogen permeation fluxes of the reduced activation ferritic steel F82H were quantitatively measured by a newly proposed method, vacuum thermo-balance method, for a precise estimation of tritium leakage in a fusion reactor. We prepared sample capsules made of F82H, which enclosed hydrogen gas. The hydrogen in the capsules permeated through the capsule wall, and subsequently desorbed from the capsule surface during isothermal heating. The vacuum thermo-balance method allows simultaneous measurement of the hydrogen permeation flux by two independent methods, namely, the net weight reduction of the sample capsule and exhaust gas analysis. Thus the simultaneous measurements by two independent methods increase the reliability of the permeability measurement. When the gas pressure of enclosed hydrogen was 0.8 atm at the sample temperature of 673 K, the hydrogen permeation flux of F82H obtained by the net weight reduction and the exhaust gas analysis was 0.75x10 18 (H 2 /m 2 s) and 2.2x10 18 (H 2 /m 2 s), respectively. The ratio of the hydrogen permeation fluxes obtained by the net weight reduction to that measured by the exhaust gas analysis was in the range from 1/4 to 1/1 in this experiment. The temperature dependence of the estimated permeation flux was similar in both methods. Taking the uncertainties of both measurements into consideration, both results are supposed to be consistent. The enhancement of hydrogen permeation flux was observed from the sample of which outer surface was mechanically polished. Through the present experiments, it has been demonstrated that the vacuum thermo-balance method is effective for the measurement of hydrogen permeation rate of F82H. (author)

  11. Structural and functional significance of water permeation through cotransporters

    DEFF Research Database (Denmark)

    Zeuthen, Thomas; Gorraitz, Edurne; Her, Ka

    2016-01-01

    Membrane transporters, in addition to their major role as specific carriers for ions and small molecules, can also behave as water channels. However, neither the location of the water pathway in the protein nor their functional importance is known. Here, we map the pathway for water and urea...... through the intestinal sodium/glucose cotransporter SGLT1. Molecular dynamics simulations using the atomic structure of the bacterial transporter vSGLT suggest that water permeates the same path as Na+ and sugar. On a structural model of SGLT1, based on the homology structure of vSGLT, we identified...... to be due to alterations in steric hindrance to water and urea, and/or changes in protein folding caused by mismatching of side chains in the water pathway. Water permeation through SGLT1 and other transporters bears directly on the structural mechanism for the transport of polar solutes through...

  12. Alfuzosin hydrochloride transdermal films: evaluation of physicochemical, in vitro human cadaver skin permeation and thermodynamic parameters

    Directory of Open Access Journals (Sweden)

    Satyanarayan Pattnaik

    2009-12-01

    Full Text Available Purpose: The main objective of the investigation was to develop a transdermal therapeutic system for alfuzosin hydrochloride and to study the effects of polymeric system and loading dose on the in vitro skin permeation pattern. Materials and methods: Principles of experimental design have been exploited to develop the dosage form. Ratio of ethyl cellulose (EC and polyvinyl pyrrolidone (PVP and loading dose were selected as independent variables and their influence on the cumulative amount of alfuzosin hydrochloride permeated per cm2 of human cadaver skin at 24 h (Q24, permeation flux (J and steady state permeability coefficient (P SS were studied using experimental design. Various physicochemical parameters of the transdermal films were also evaluated. Activation energy for in vitro transdermal permeation has been estimated. Results: Ratio of EC and PVP was found to be the main influential factor for all the dependent variables studied. Drug loading dose was also found to influence the dependent variables but to a lesser extent. Physicochemical parameters of the prepared films were evaluated and found satisfactory. Activation energy for alfuzosin permeation has also been estimated and reported. Conclusion: The therapeutic system was found to be dermatologically non-irritant and hence, a therapeutically effective amount of alfuzosin hydrochloride can be delivered via a transdermal route.

  13. Liquid crystalline systems for transdermal delivery of celecoxib: in vitro drug release and skin permeation studies.

    Science.gov (United States)

    Estracanholli, Eder André; Praça, Fabíola Silva Garcia; Cintra, Ana Beatriz; Pierre, Maria Bernadete Riemma; Lara, Marilisa Guimarães

    2014-12-01

    Liquid crystalline systems of monoolein/water could be a promising approach for the delivery of celecoxib (CXB) to the skin because these systems can sustain drug release, improve drug penetration into the skin layers and minimize side effects. This study evaluated the potential of these systems for the delivery of CXB into the skin based on in vitro drug release and skin permeation studies. The amount of CXB that permeated into and/or was retained in the skin was assayed using an HPLC method. Polarizing light microscopy studies showed that liquid crystalline systems of monoolein/water were formed in the presence of CXB, without any changes in the mesophases. The liquid crystalline systems decreased drug release when compared to control solution. Drug release was independent of the initial water content of the systems and CXB was released from cubic phase systems, irrespective of the initial water content. The systems released the CXB following zero-order release kinetics. In vitro drug permeation studies showed that cubic phase systems allowed drug permeation and retention in the skin layers. Cubic phase systems of monoolein/water may be promising vehicles for the delivery of CXB in/through the skin because it improved CXB skin permeation compared with the control solution.

  14. Comparison of Hexane Vapour Permeation in Two Different Polymeric Membranes via an Innovative In-line FID Detection Method

    Directory of Open Access Journals (Sweden)

    Z. Petrusová

    2017-07-01

    Full Text Available This manuscript presents a novel method for the analysis of vapour permeation through polymeric membranes based on in-line analysis of the permeate with an FID detector. The hexane vapour permeation was studied for two commercially available membranes, namely low-density polyethylene (LDPE and thin-film-composite polyamide (PA membrane. The hexane permeation was studied at temperatures of 25–45 °C, hexane vapour activity in the range of 0.2–0.8 and trans-membrane pressures of 5–50 kPa. Two fundamentally different membranes were chosen to demonstrate the potential and sensitivity of the permeation apparatus. Upon increasing the temperature from 25 to 45 °C, the flux in LDPE was found to increase almost fourfold over the whole activity range. The nonlinear increase of the flux with activity indicates plasticization of the polymer by hexane. Contrarily, the flux in the PA membrane increases almost linearly with activity, with only a minor upward curvature. Since the PA is far away from any phase transition, it is less temperature-dependent than LDPE. The activation energy for permeation demonstrates that the temperature dependence in the LDPE membrane is dominated by changes in diffusion, whereas it is dominated by changes in solubility in the PA membrane.

  15. Reduction of tritium permeation through Inconel 718 and Incoloy 800 HT by means of natural oxides

    Energy Technology Data Exchange (ETDEWEB)

    Aiello, A., E-mail: antonio.aiello@enea.it [ENEA C.R. Brasimone, I-40032 Camugnano (Italy); Utili, M.; Ciampichetti, A. [ENEA C.R. Brasimone, I-40032 Camugnano (Italy)

    2011-10-01

    Chronical releases of tritium from the helium primary coolant into the water secondary coolant is a fundamental safety issue in the design of a fusion reactor steam generator. It is well known that the steam/water circuit of a fusion reactor would be considered not relevant from a radiological point of view, while if a strong permeation of tritium will be present it will be released together with incondensable gases in the condenser. The permeation of hydrogen isotopes through candidate steam generator materials in different conditions was studied in the past. Further experiments demonstrated that nickel alloys of nuclear interest are always covered by a thin and adherent oxide layer able to reduce permeation of orders of magnitude. The major objective of this work is the evaluation of the permeated flux through nickel alloys, when exposed to pure hydrogen and to an oxidant gas stream, to verify the real permeability of these materials in conditions close to those foreseen in the helium side of the steam generator.

  16. Tritium breeders and tritium permeation barrier coatings for fusion reactor

    International Nuclear Information System (INIS)

    Yamawaki, Michio; Kawamura, Hiroshi; Tsuchiya, Kunihiko

    2004-01-01

    A state of R and D of tritium breeders and tritium permeation barrier coatings for fusion reactor is explained. A list of candidate for tritium breeders consists of ceramics containing lithium, for examples, Li 2 O, Li 2 TiO 3 , Li 2 ZrO 3 , Li 4 SiO 4 and LiAlO 2 . The characteristics and form are described. The optimum particle size is from 1 to 10 μm. The production technologies of tritium breeders in the world are stated. Characteristics of ceramics with lithium as tritium breeders are compared. TiC, TiN/TiC, Al 2 O 3 and Cr 2 O 3 -SiO 2 -P 2 O 5 are tritium permeation barrier coating materials. These production methods and evaluation of characteristics are explained. (S.Y.)

  17. Protection efficacy of gloves against components of the solvent in a sprayed isocyanate coating utilizing a reciprocating permeation panel.

    Science.gov (United States)

    Ceballos, Diana M; Reeb-Whitaker, Carolyn; Sasakura, Miyoko; Dills, Russell; Yost, Michael G

    2015-04-01

    Determine protection effectiveness of 5-mil natural rubber latex (0.13-mm), 5-mil nitrile rubber (0.13-mm), and 13-mil butyl rubber (0.33-mm) glove materials against solvents present in a commonly used automotive clear coat formulation using a novel permeation panel. The latex and nitrile gloves were the type commonly used by local autobody spray painters. Glove materials were tested by spraying an automotive clear coat onto an automated reciprocating permeation panel (permeation panel II). Temperature, relative humidity, and spray conditions were controlled to optimize clear coat loading homogeneity as evaluated by gravimetric analysis. Solvent permeation was measured using charcoal cloth analyzed by the National Institute for Occupational Safety and Health 1501 method. Natural rubber latex allowed 3-5 times the permeation of solvents relative to nitrile rubber for all 10 solvents evaluated: ethyl benzene, 2-heptanone, 1-methoxy-2-propyl acetate, o-xylene, m-xylene, p-xylene, n-butyl acetate, methyl isobutyl ketone, petroleum distillates, and toluene. There is a distinct behavior in solvent permeation before and after the coating dry time. Solvent permeation increased steadily before coating dry time and remained fairly constant after coating dry time. Butyl was not permeated by any of the solvents under the conditions tested. Commonly used 5-mil thick (0.13-mm) latex and nitrile gloves were ineffective barriers to solvents found in a commonly used clear coat formulation. Conversely, 13-mil (0.33-mm) butyl gloves were found to be protective against all solvents in the clear coat formulation. © The Author 2014. Published by Oxford University Press on behalf of the British Occupational Hygiene Society.

  18. Study of tritium permeation through Peach Bottom Steam Generator tubes

    International Nuclear Information System (INIS)

    Yang, L.; Baugh, W.A.; Baldwin, N.L.

    1977-06-01

    The report describes the equipment developed, samples tested, procedures used, and results obtained in the tritium permeation tests conducted on steam generator tubing samples which were removed from the Peach Bottom Unit No. 1 reactor

  19. Analysis on tritium permeation in tritium storage bed with gas flowing calorimetry

    Energy Technology Data Exchange (ETDEWEB)

    Nakamura, Hirofumi; Hayashi, Takumi; Suzuki, Takumi; Nishi, Masataka [Japan Atomic Energy Research Inst., Naka Fusion Research Establishment, Department of Fusion Engineering Research, Naka, Ibaraki (Japan); Yoshida, Hiroshi [Japan Atomic Energy Research Inst., Naka Fusion Research Establishment, ITER-Joint Centeral Team, Naka, Ibaraki (Japan)

    2000-10-01

    Tritium permeation amount in a tritium storage bed with gas flowing calorimetric was evaluated under a condition of new operation mode for International Thermonuclear Experimental Reactor (ITER). As a result, tritium permeation under the new operation mode was estimated to be about twice of that under the practical operation mode. This result show that it would be regardless in a view point of material control of tritium, however, it was suggested to be required additional tritium removal or evacuate system in a view points of safety control or performance of accountability or thermal insulating of the tritium storage bed. (author)

  20. Nitride and carbide thin films as hydrogen permeation barrier on Manet steel

    International Nuclear Information System (INIS)

    Benamati, G.; Checchetto, R.; Bonelli, M.; Gratton, L.M.; Guzman, L.; Miotello, A.; Terlain, A.

    1995-01-01

    TiC / TiN bilayers, ∼ 1.2 μm thick, were deposited on Manet II steel by the ion beam assisted deposition technique to investigate the possible use of this ceramic coating as hydrogen barrier. Hydrogen permeation experiments in the temperature range 470-570 K showed indeed that this coating is a very efficient barrier to the hydrogen permeation being able to reduce the hydrogen flux up to two order of magnitude with respect to the uncoated steel. Preliminary compatibility tests between coated Manet II and Pb-17Li showed no attack of Pb-17Li to the steel. (orig.)

  1. A description of an ultra high vacuum device for permeation measurements

    International Nuclear Information System (INIS)

    Gautsch, O.; Hodapp, G.

    1984-01-01

    The permeability of deuterium through austenitic stainless steel membranes is determined by means of an UHV-apparatus as a function of temperature, membrane thickness, and deuterium upstream driving pressure. The upstream side of the membrane is in contact with the liquid alloy of the eutectic Pb 83 Li 17 in order to represent the situation of a tritium breeding blanket of a fusion reactor. By means of the experimental results the amounts of tritium which permeate across the walls of the cooling tubes into the coolant will be determined. The purpose of this work is to describe the apparatus and the methods used for the determination of permeabilities. Also some preliminary results of permeation flux measurements are given

  2. Transdermal delivery of carvedilol containing glycyrrhizin and chitosan as permeation enhancers: biochemical, biophysical, microscopic and pharmacodynamic evaluation.

    Science.gov (United States)

    Sapra, Bharti; Jain, Subheet; Tiwary, A K

    2008-09-01

    The present study was aimed at unveiling the influence of glycyrrhizin and chitosan on rat epidermis and to correlate these effects with percutaneous permeation characteristics of carvedilol. The permeation of carvedilol across excised rat epidermis was significantly higher (p vehicle as compared to propylene glycol:ethanol (7:3) mixture. Epidermis obtained after 12 hr treatment of viable rat skin with a glycyrrhizin-chitosan mixture showed significantly higher (p space, disordered lipid structure and corneocyte detachment as observed in SEM and TEM suggests great potential of glycyrrhizin for use as a percutaneous permeation enhancer.

  3. Preparation, characterization and permeation studies of a nanovesicular system containing diclofenac for transdermal delivery.

    Science.gov (United States)

    Gaur, Praveen Kumar; Purohit, Suresh; Kumar, Yatendra; Mishra, Shikha; Bhandari, Anil

    2014-02-01

    Transdermal formulations contain permeation enhancer which causes skin damage. Ceramide 2 is natural lipid found in stratum corneum (SC). Drug-loaded nanovesicles of ceramide-2, cholesterol, palmitic acid, cholesteryl sulfate were formulated and analyzed for physical and biological properties. Diclofenac was used as a model drug. The vesicles were prepared using the film hydration method and characterized for physical parameters, in vitro drug release, accelerated stability studies and formulated into gel. Respective gels were compared with a commercial formulation (CEG) and plain carbopol gel (CG) containing drug for ex vivo, in vivo drug permeation and anti-inflammatory activity. The vesicles were stable with optimum physical parameters. DCG-1 showed 92.89% in vitro drug release. Ceramide vesicles showed drug release between 18 and 25 μg/cm(2) whereas CG and CEG released 0.33 and 1.35 μg/cm(2) drug, respectively. DCG-1 and CEG showed corresponding Cmax at 6 and 4 h, respectively. DCG-1 showed six times AUC than CEG. DCG-1 inhibited edema by 86.37% by 4th hour of application. The presence of ceramide 2 specifically promotes the drug permeation through SC and dermis and also contribute towards stability and non-irritancy. The composition of the nanovesicle played an important role in physical properties and drug permeation.

  4. Buccal delivery of thiocolchicoside: in vitro and in vivo permeation studies.

    Science.gov (United States)

    Artusi, M; Santi, P; Colombo, P; Junginger, H E

    2003-01-02

    Thiocolchicoside, a muscle-relaxant agent, is administered by the oral, intra-muscular and topical route. After oral administration the extent of bioavailability compared with intra-muscular administration is low, due to a first pass effect. In this paper, the delivery of thiocolchicoside through oral mucosa is studied to improve the bioavailability. Thiocolchicoside in vitro permeation through porcine oral mucosa and in vivo buccal transport in humans were investigated. Two dosage forms, a bioadhesive disc and a fast dissolving disc for buccal and sublingual administration of thiocolchicoside, respectively, were designed. The in vitro permeation of thiocolchicoside through porcine buccal mucosa from these dosage forms was evaluated and compared with in vivo absorption. Results from in vitro studies demonstrated that thiocolchicoside is quite permeable across porcine buccal mucosa and that permeation enhancers, such as sodium taurocholate and sodium taurodeoxycholate, were not able to increase its flux. The in vivo thiocolchicoside absorption experiments, in which the drug loss from oral cavity was measured, indicated that both formulations could be useful for therapeutic application. The fast dissolving (sublingual) form resulted in a quick uptake of 0.5 mg of thiocolchicoside within 15 min whereas with the adhesive buccal form the same dose can be absorbed over an extended period of time.

  5. Acquisition of Preparative Gel Permeation Chromatography for Research and Education in Energy Conversion and Nanocomposites

    Science.gov (United States)

    2017-04-19

    AFRL-AFOSR-VA-TR-2017-0090 Acquisition of Preparative Gel Permeation Chromatography for Research and Education in Energy Conversion and...TITLE AND SUBTITLE Acquisition of Preparative Gel Permeation Chromatography for Research and Education in Energy Conversion and Nanocomposites 5a... research with education at all levels across a broad range of materials, and create important opportunities to expose and train undergraduates, women

  6. Interactions between oxygen permeation and homogeneous-phase fuel conversion on the sweep side of an ion transport membrane

    KAUST Repository

    Hong, Jongsup

    2013-02-01

    The interactions between oxygen permeation and homogeneous fuel oxidation reactions on the sweep side of an ion transport membrane (ITM) are examined using a comprehensive model, which couples the dependency of the oxygen permeation rate on the membrane surface conditions and detailed chemistry and transport in the vicinity of the membrane. We assume that the membrane surface is not catalytic to hydrocarbon or syngas oxidation. Results show that increasing the sweep gas inlet temperature and fuel concentration enhances oxygen permeation substantially. This is accomplished through promoting oxidation reactions (oxygen consumption) and the transport of the products and reaction heat towards the membrane, which lowers the oxygen concentration and increases the gas temperature near the membrane. Faster reactions at higher fuel concentration and higher inlet gas temperature support substantial fuel conversion and lead to a higher oxygen permeation flux without the contribution of surface catalytic activity. Beyond a certain maximum in the fuel concentration, extensive heat loss to the membrane (and feed side) reduces the oxidation kinetic rates and limits oxygen permeation as the reaction front reaches the membrane. The sweep gas flow rate and channel height have moderate impacts on oxygen permeation and fuel conversion due to the residence time requirements for the chemical reactions and the location of the reaction zone relative to the membrane surface. © 2012 Elsevier B.V.

  7. Fabrication, appraisal, and transdermal permeation of sildenafil citrate-loaded nanostructured lipid carriers versus solid lipid nanoparticles

    Science.gov (United States)

    Elnaggar, Yosra SR; El-Massik, Magda A; Abdallah, Ossama Y

    2011-01-01

    Although sildenafil citrate (SC) is used extensively for erectile dysfunction, oral delivery of SC encounters many obstacles. Furthermore, the physicochemical characteristics of this amphoteric drug are challenging for delivery system formulation and transdermal permeation. This article concerns the assessment of the potential of nanomedicine for improving SC delivery and transdermal permeation. SC-loaded nanostructured lipid carriers (NLCs) and solid lipid nanoparticles (SLNs) were fabricated using a modified high-shear homogenization technique. Nanoparticle optimization steps included particle size analysis, entrapment efficiency (EE) determination, freeze-drying and reconstitution, differential scanning calorimetry, in vitro release, stability study and high-performance liquid chromatography analysis. Transdermal permeation of the nanocarriers compared with SC suspension across human skin was assessed using a modified Franz diffusion cell assembly. Results revealed that SLNs and NLCs could be optimized in the nanometric range (180 and 100 nm, respectively) with excellent EE (96.7% and 97.5%, respectively). Nanoparticles have significantly enhanced in vitro release and transdermal permeation of SC compared with its suspensions. Furthermore, transdermal permeation of SC exhibited higher initial release from both SLN and NLC formulations followed by controlled release, with promising implications for faster onset and longer drug duration. Nanomedicines prepared exhibited excellent physical stability for the study period. Solid nanoparticles optimized in this study successfully improved SC characteristics, paving the way for an efficient topical Viagra® product. PMID:22238508

  8. Skin Delivery of EGCG and Silibinin: Potential of Peptide Dendrimers for Enhanced Skin Permeation and Deposition.

    Science.gov (United States)

    Shetty, Pallavi Krishna; Manikkath, Jyothsna; Tupally, Karnaker; Kokil, Ganesh; Hegde, Aswathi R; Raut, Sushil Y; Parekh, Harendra S; Mutalik, Srinivas

    2017-08-01

    The aim of the present study was to evaluate the ability of the peptide dendrimers to facilitate transdermal delivery of antioxidants, silibinin, and epigallocatechin-3-gallate (EGCG). Drug-peptide dendrimer complexes were prepared and evaluated for their ability to permeate across the skin. The data revealed the ready formation of complexes between drug and peptide dendrimer in a molar ratio of 1:1. In vitro permeation studies using excised rat skin and drug-peptide dendrimer complexes showed highest values for cumulative drug permeation at the end of 12 h (Q 12 ), with corresponding permeability coefficient (Kp) and enhancement ratio values also determined at this time point. With silibinin, 3.96-, 1.81-, and 1.06-fold increase in skin permeation was observed from silibinin-peptide dendrimer complex, simultaneous application of silibinin + peptide dendrimer, and pretreatment of skin with peptide dendrimer, respectively, in comparison with passive diffusion. With EGCG, 9.82-, 2.04-, and 1.72-fold increase in skin permeation was observed from EGCG-peptide dendrimer complex, simultaneous application of EGCG + peptide dendrimer, and pretreatment of skin with peptide dendrimer, respectively, in comparison with passive diffusion. The present study demonstrates the application of peptide dendrimers in effectively delivering antioxidants such as EGCG and silibinin into the skin, thus offering the potential to provide antioxidant effects when delivered via appropriately formulated topical preparations.

  9. Excessive daytime sleepiness and metabolic syndrome in men with obstructive sleep apnea: a large cross-sectional study.

    Science.gov (United States)

    Fu, Yiqun; Xu, Huajun; Xia, Yunyan; Qian, Yingjun; Li, Xinyi; Zou, Jianyin; Wang, Yuyu; Meng, Lili; Tang, Xulan; Zhu, Huaming; Zhou, Huiqun; Su, Kaiming; Yu, Dongzhen; Yi, Hongliang; Guan, Jian; Yin, Shankai

    2017-10-03

    Excessive daytime sleepiness is a common symptom in obstructive sleep apnea (OSA). Previous studies have showed that excessive daytime sleepiness is associated with some individual components of metabolic syndrome. We performed a large cross-sectional study to explore the relationship between excessive daytime sleepiness and metabolic syndrome in male OSA patients. A total of 2241 suspected male OSA patients were consecutively recruited from 2007 to 2013. Subjective daytime sleepiness was assessed using the Epworth sleepiness scale. Anthropometric, metabolic, and polysomnographic parameters were measured. Metabolic score was used to evaluate the severity of metabolic syndrome. Among the male OSA patients, most metabolic parameters varied by excessive daytime sleepiness. In the severe group, male OSA patients with excessive daytime sleepiness were more obese, with higher blood pressure, more severe insulin resistance and dyslipidemia than non-sleepy patients. Patients with metabolic syndrome also had a higher prevalence of excessive daytime sleepiness and scored higher on the Epworth sleepiness scale. Excessive daytime sleepiness was independently associated with an increased risk of metabolic syndrome (odds ratio =1.242, 95% confidence interval: 1.019-1.512). No substantial interaction was observed between excessive daytime sleepiness and OSA/ obesity. Excessive daytime sleepiness was related to metabolic disorders and independently associated with an increased risk of metabolic syndrome in men with OSA. Excessive daytime sleepiness should be taken into consideration for OSA patients, as it may be a simple and useful clinical indicator for evaluating the risk of metabolic syndrome.

  10. Protein permeation through polymer membranes for hybrid-type artificial pancreas

    Energy Technology Data Exchange (ETDEWEB)

    Burczak, K; Fujisato, Toshiya; Ikada, Yoshito [Kyoto Univ. (Japan); Hatada, Motoyoshi

    1991-05-01

    Hydrogel membranes were prepared by radiation crosslinking of poly (vinyl alcohol) (PVA) in aqueous solutions. Effects of PVA concentration, PVA molecular weight, and radiation dose on the permeation of insulin and immunoglobulin through the membranes were investigated. Glucose permeation was also studied. The crosslinking density affected the size of macromolecular mesh of hydrogel network as well as the water content of membrane responsible for the diffusion of the solutes. The diffusion coefficient linearly increased for all the solutes with the increasing water content in PVA hydrogels, indicating that diffusion occurs primarily through the water hydrating the polymer network. The increase in crosslinking density of hydrogels by changing PVA molecular weight brought about the decrease in mesh size of the hydrogels, which, in turn, had an influence on the diffusion of immunoglobulin, but not of insulin and glucose. (author).

  11. Natural oils as skin permeation enhancers for transdermal delivery of olanzapine: in vitro and in vivo evaluation.

    Science.gov (United States)

    Aggarwal, Geeta; Dhawan, Sanju; HariKumar, S L

    2012-03-01

    The feasibility of development of transdermal delivery system of olanzapine utilizing natural oils as permeation enhancers was investigated. Penetration enhancing potential of corn (maize) oil, groundnut oil and jojoba oil on in vitro permeation of olanzapine across rat skin was studied. The magnitude of flux enhancement factor with corn oil, groundnut oil and jojoba oil was 7.06, 5.31 and 1.9 respectively at 5mg/ml concentration in solvent system. On the basis of in vitro permeation studies, eudragit based matrix type transdermal patches of olanzapine were fabricated using optimized concentrations of natural oils as permeation enhancers. All transdermal patches were found to be uniform with respect to physical characteristics. The interaction studies carried out by comparing the results of ultraviolet, HPLC and FTIR analyses for the pure drug, polymers and mixture of drug and polymers indicated no chemical interaction between the drug and excipients. Corn oil containing unsaturated fatty acids was found to be promising natural permeation enhancer for transdermal delivery of olanzapine with greatest cumulative amount of drug permeated (1010.68 μg/cm²/h) up to 24 h and caused no skin irritation. The fabricated transdermal patches were found to be stable. The pharmacokinetic characteristics of the final optimized matrix patch (T2) were determined after transdermal application to rabbits. The calculated relative bioavailability of TDDS was 113.6 % as compared to oral administration of olanzapine. The therapeutic effectiveness of optimized transdermal system was confirmed by tranquillizing activity in rotarod and grip mice model.

  12. Permeation enhancing polymers in oral delivery of hydrophilic macromolecules: thiomer/GSH systems.

    Science.gov (United States)

    Bernkop-Schnürch, A; Kast, C E; Guggi, D

    2003-12-05

    Thiolated polymers (= thiomers) in combination with reduced glutathione (GSH) were shown to improve the uptake of hydrophilic macromolecules from the GI tract. The mechanism responsible for this permeation enhancing effect seems to be based on the thiol groups of the polymer. These groups inhibit protein tyrosine phosphatase, being involved in the closing process of tight junctions, via a GSH-mediated mechanism. The strong permeation enhancing effect of various thiomer/GSH systems such as poly(acrylic acid)-cysteine/GSH or chitosan-4-thio-butylamidine (chitosan-TBA)/GSH could be shown via permeation studies on freshly excised intestinal mucosa in Ussing-type chambers. Furthermore, the efficacy of the system was also shown in vivo. By utilizing poly(acrylic acid)-cysteine/GSH as carrier matrix, an absolute oral bioavailability for low molecular weight heparin of 19.9 +/- 9.3% and a pharmacological efficacy--calculated on the basis of the areas under the reduction in serum glucose levels of the oral formulation versus subcutaneous (s.c.) injection-for orally given insulin of 7% could be achieved. The incorporation of salmon calcitonin in chitosan-TBA/GSH led on the other hand to a pharmacological efficacy based on the areas under the reduction in plasma calcium levels of the oral thiomer formulation versus intravenous (i.v.) injection of 1.3%. Because of this high efficacy (i), the possibility to combine thiomer/GSH systems with additional low molecular weight permeation enhancers acting in other ways (ii) and minimal toxicological risks as these polymers are not absorbed from the GI tract (iii), thiolated polymers represent a promising novel tool for the oral administration of hydrophilic macromolecules.

  13. Characterization and utilization of the permeate and retentate obtained after “dead-end” ultrafiltration

    Directory of Open Access Journals (Sweden)

    Vasić Vesna M.

    2013-01-01

    Full Text Available In the recent years, with the increase in bioethanol production, the increasing amounts of distillery wastewater are generated. Such wastewater (stillage is one of the most polluted waste product of the food and beverage industries. The present study evaluates the treatment of distillery wastewater by ultrafiltration (UF, in order to reduce its pollution and evaluate the composition of the permeate and retentate. Polyethersulfone ultrafiltration membrane with molecular weight cut-off (MWCO 30000 Da, was used for the experiments. The UF was carried out in dead-end mode. The results of the analyses of the permeate and retentate obtained after ultrafiltration were considered as well as different ways for their further utilization. The pollutant level in the permeate was decreased significantly in comparison to the raw stillage, and suspended solids were completely removed from the stillage. [Projekat Ministarstva nauke Republike Srbije, br. TR 31002

  14. Preparation and Skin Permeation Study of N, N- Diethyl- meta-Toluamide Semi Solid Formulations

    Directory of Open Access Journals (Sweden)

    Solmaz Ghaffari

    2013-06-01

    Full Text Available N,N-Diethyl meta Toluamide (DEET is an insect repellent agent that contrary to its benefits, if is used in formulations with high skin permeation, will produce side effects of different severity. This study attempted to achieve a semi-solid DEET containing formulation with good appearance, sufficient spreadity, suitable viscosity for tube and jar filling, compatible pH with skin, reasonable stability, longer release time, and the less skin permeation. To obtain such a formulation, three types of DEET containing semi solids including gels (hydrophile, creams (emulsion and ointments (lipophile, and their characteristics were compared with each other and with Off! Brand. Results showed that one of the prepared creams with the proper viscosity, stability, appearance and spreadity, had the least drug release in six hours and less skin permeation of DEET as compared with Off!. Hence the preparation was introduced as the optimal formulation.

  15. Influence of adsorbed hydrogen molecules on the hydrogen permeation and recycling

    International Nuclear Information System (INIS)

    Banno, T.

    1987-01-01

    Hydrogen permeation through a thin iron membrane has been measured using a molecular hydrogen gas and/or a weakly ionized rf-discharge as particle sources. Transient and steady state values of the permeation flux φ p were obtained in the regime where the surface penetration of H and the recombinative release of H 2 are rate-determining. φ p is then proportional to the flux density ψ of the particles which impinge on the membrane upstream. The proportionality factors depend on the nature (atomic or molecular) of the incident particles, on temperature (in particular for a molecular driving gas) and on the ratio of the surface recombinative release rate constants (2σk r ) u and (2σk r ) d up- and downstream, respectively. (orig.)

  16. Characterization of Thin Film Polymers Through Dynamic Mechanical Analysis and Permeation

    Science.gov (United States)

    Herring, Helen

    2003-01-01

    Thin polymer films are being considered, as candidate materials to augment the permeation resistance of cryogenic hydrogen fuel tanks such as would be required for future reusable launch vehicles. To evaluate performance of candidate films after environmental exposure, an experimental study was performed to measure the thermal/mechanical and permeation performance of six, commercial-grade materials. Dynamic storage modulus, as measured by Dynamic Mechanical Analysis, was found over a range of temperatures. Permeability, as measured by helium gas diffusion, was found at room temperature. Test data was correlated with respect to film type and pre-test exposure to moisture, elevated temperature, and cryogenic temperature. Results indicated that the six films were comparable in performance and their resistance to environmental degradation.

  17. Silicon Carbide as a tritium permeation barrier in tungsten plasma-facing components

    Science.gov (United States)

    Wright, G. M.; Durrett, M. G.; Hoover, K. W.; Kesler, L. A.; Whyte, D. G.

    2015-03-01

    The control of tritium inventory is of great importance in future fusion reactors, not only from a safety standpoint but also to maximize a reactor's efficiency. Due to the high mobility of hydrogenic species in tungsten (W) one concern is the loss of tritium from the system via permeation through the tungsten plasma-facing components (PFC). This can lead to loss of tritium through the cooling channels of the wall thereby mandating tritium monitoring and recovery methods for the cooling system of the first wall. The permeated tritium is then out of the fuel cycle and cannot contribute to energy production until it is recovered and recycled into the system.

  18. Nitride and carbide thin films as hydrogen permeation barrier on MANET steel

    International Nuclear Information System (INIS)

    Benamati, G.

    1994-01-01

    TiC/TiN bilayers, - 1.2 μm thick, were deposited on Manet II steel by the ion beam assisted deposition technique to investigate the possible use of this ceramic coating as hydrogen barrier. Hydrogen permeation experiments in the temperature range 470-570 K showed indeed that this coating is a very efficient barrier to the hydrogen permeation being able to reduce the hydrogen flux up to two order of magnitude with respect to the uncoated steel. Preliminary compatibility tests between coated Manet II and Pb-17Li showed no attack of Pb-17Li to the steel. (author) 8 refs.; 2 figs.; 1 tab

  19. Mutations in the voltage-sensing domain affect the alternative ion permeation pathway in the TRPM3 channel.

    Science.gov (United States)

    Held, Katharina; Gruss, Fabian; Aloi, Vincenzo Davide; Janssens, Annelies; Ulens, Chris; Voets, Thomas; Vriens, Joris

    2018-03-31

    Mutagenesis at positively charged amino acids (arginines and lysines) (R1-R4) in the voltage-sensor domain (transmembrane segment (S) 4) of voltage-gated Na + , K + and Ca 2+ channels can lead to an alternative ion permeation pathway distinct from the central pore. Recently, a non-canonical ion permeation pathway was described in TRPM3, a member of the transient receptor potential (TRP) superfamily. The non-canonical pore exists in the native TRPM3 channel and can be activated by co-stimulation of the endogenous agonist pregnenolone sulphate and the antifungal drug clotrimazole or by stimulation of the synthetic agonist CIM0216. Alignment of the voltage sensor of Shaker K + channels with the entire TRPM3 sequence revealed the highest degree of similarity in the putative S4 region of TRPM3, and suggested that only one single gating charge arginine (R2) in the putative S4 region is conserved. Mutagenesis studies in the voltage-sensing domain of TRPM3 revealed several residues in the voltage sensor (S4) as well as in S1 and S3 that are crucial for the occurrence of the non-canonical inward currents. In conclusion, this study provides evidence for the involvement of the voltage-sensing domain of TRPM3 in the formation of an alternative ion permeation pathway. Transient receptor potential (TRP) channels are cationic channels involved in a broad array of functions, including homeostasis, motility and sensory functions. TRP channel subunits consist of six transmembrane segments (S1-S6), and form tetrameric channels with a central pore formed by the region encompassing S5 and S6. Recently, evidence was provided for the existence of an alternative ion permeation pathway in TRPM3, which allows large inward currents upon hyperpolarization independently of the central pore. However, very little knowledge is available concerning the localization of this alternative pathway in the native TRPM3 channel protein. Guided by sequence homology with Shaker K + channels, in which

  20. Incorporation of whey permeate, a dairy effluent, in ethanol fermentation to provide a zero waste solution for the dairy industry.

    Science.gov (United States)

    Parashar, Archana; Jin, Yiqiong; Mason, Beth; Chae, Michael; Bressler, David C

    2016-03-01

    This study proposes a novel alternative for utilization of whey permeate, a by-product stream from the dairy industry, in wheat fermentation for ethanol production using Saccharomyces cerevisiae. Whey permeates were hydrolyzed using enzymes to release fermentable sugars. Hydrolyzed whey permeates were integrated into wheat fermentation as a co-substrate or to partially replace process water. Cold starch hydrolysis-based simultaneous saccharification and fermentation was done as per the current industrial protocol for commercial wheat-to-ethanol production. Ethanol production was not affected; ethanol yield efficiency did not change when up to 10% of process water was replaced. Lactic acid bacteria in whey permeate did not negatively affect the co-fermentation or reduce ethanol yield. Whey permeate could be effectively stored for up to 4 wk at 4 °C with little change in lactose and lactic acid content. Considering the global abundance and nutrient value of whey permeate, the proposed strategy could improve economics of the dairy and biofuel sectors, and reduce environmental pollution. Furthermore, our research may be applied to fermentation strategies designed to produce value-added products other than ethanol. Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  1. Development of a low tritium partial pressure permeation system for mass transport measurement in lead lithium eutectic

    International Nuclear Information System (INIS)

    Pawelko, R.; Shimada, M.; Katayama, K.; Fukada, S.; Terai, T.

    2014-01-01

    A new experimental system designed to investigate tritium mass transfer properties in materials important to fusion technology is operational at the Safety and Tritium Applied Research (STAR) facility located at the Idaho National Laboratory (INL). The tritium permeation measurement system was developed as part of the Japan/US TITAN collaboration to investigate tritium mass transfer properties in liquid lead lithium eutectic (LLE) alloy. The system is similar to a hydrogen/deuterium permeation measurement system developed at Kyushu University and also incorporates lessons learned from previous tritium permeation experiments conducted at the STAR facility. This paper describes the experimental system that is configured specifically to measure tritium mass transfer properties at low tritium partial pressures. We present preliminary tritium permeation results for α-Fe and α-Fe/LLE samples at 600degC and at tritium partial pressures between 1.0E-3 and 2.4 Pain helium. The preliminary results are compared with literature data. (author)

  2. Effect of Enhancers on in vitro and in vivo Skin Permeation and Deposition of S-Methyl-L-Methionine.

    Science.gov (United States)

    Kim, Ki Taek; Kim, Ji Su; Kim, Min-Hwan; Park, Ju-Hwan; Lee, Jae-Young; Lee, WooIn; Min, Kyung Kuk; Song, Min Gyu; Choi, Choon-Young; Kim, Won-Serk; Oh, Hee Kyung; Kim, Dae-Duk

    2017-07-01

    S-methyl- L -methionine (SMM), also known as vitamin U, is commercially available as skin care cosmetic products for its wound healing and photoprotective effects. However, the low skin permeation expected of SMM due to its hydrophilic nature with a log P value of -3.3, has not been thoroughly addressed. The purpose of this study thus was to evaluate the effect of skin permeation enhancers on the skin permeation/deposition of SMM. Among the enhancers tested for the in vitro skin permeation and deposition of SMM, oleic acid showed the most significant enhancing effect. Moreover, the combination of oleic acid and ethanol further enhanced in vitro permeation and deposition of SMM through hairless mouse skin. Furthermore, the combination of oleic acid and ethanol significantly increased the in vivo deposition of SMM in the epidermis/dermis for 12 hr, which was high enough to exert a therapeutic effect. Therefore, based on the in vitro and in vivo studies, the combination of oleic acid and ethanol was shown to be effective in improving the topical skin delivery of SMM, which may be applied in the cosmetic production process for SMM.

  3. A study of hydrogen permeation in aluminum alloy treated by various oxidation processes

    International Nuclear Information System (INIS)

    Song Wenhai; Long Bin

    1997-01-01

    A set of oxide coatings was formed on the surface of an Al alloy (wt%: Fe, 0.24; Si, 1.16; Cu, 0.05-0.2; Zn, 0.1; Al, residual) by means of various oxidation processes. The hydrogen permeability through the aluminum alloy and its coating materials was determined by a vapor phase permeation technique at temperatures ranging from 400 to 500 C using high-purity H 2 (99.9999%) gas with an upstream hydrogen pressure of 10 4 -10 5 Pa. The experimental results show that the hydrogen permeability through aluminum oxide coating is 100-2000 times lower than that through the aluminum alloy substrate. This means that the aluminum oxide is a significant hydrogen permeation barrier. A high hydrogen permeation resistance was observed in an oxide layer prefilmed in 200 C water, while an anodized aluminum oxide film had a less obstructive effect, possibly caused by the porous structure of the anodic oxide. The hydrogen permeability through films of aluminum oxide was not a simple function of the aluminum-oxide phase configuration. (orig.)

  4. Cutaneous Permeation and Penetration of Sunscreens: Formulation Strategies and In Vitro Methods

    Directory of Open Access Journals (Sweden)

    Silvia Tampucci

    2017-12-01

    Full Text Available Sunscreens are the most common products used for skin protection against the harmful effects of ultraviolet radiation. However, as frequent application is recommended, the use of large amount of sunscreens could reflect in possible systemic absorption and since these preparations are often applied on large skin areas, even low penetration rates can cause a significant amount of sunscreen to enter the body. An ideal sunscreen should have a high substantivity and should neither penetrate the viable epidermis, the dermis and the systemic circulation, nor in hair follicle. The research of methods to assess the degree of penetration of solar filters into the skin is nowadays even more important than in the past, due to the widespread use of nanomaterials and the new discoveries in cosmetic formulation technology. In the present paper, different in vitro studies, published in the last five years, have been reviewed, in order to focus the attention on the different methodological approaches employed to effectively assess the skin permeation and retention of sunscreens.

  5. Influence of silicon on hot-dip aluminizing process and subsequent oxidation for preparing hydrogen/tritium permeation barrier

    Energy Technology Data Exchange (ETDEWEB)

    Han, Shilei; Li, Hualing; Wang, Shumao; Jiang, Lijun; Liu, Xiaopeng [Energy Materials and Technology Research Institute, General Research Institute for Nonferrous Metals, Beijing 100088 (China)

    2010-04-15

    The development of the International Thermonuclear Experimental Reactor (ITER) requires the production of a material capable of acting as a hydrogen/tritium permeation barrier on low activation steel. It is well known that thin alumina layer can reduce the hydrogen permeation rate by several orders of magnitude. A technology is introduced here to form a ductile Fe/Al intermetallic layer on the steel with an alumina over-layer. This technology, consisting of two main steps, hot-dip aluminizing (HDA) and subsequent oxidation behavior, seems to be a promising coating method to fulfill the required goals. According to the experiments that have been done in pure Al, the coatings were inhomogeneous and too thick. Additionally, a large number of cracks and porous band could be observed. In order to solve these problems, the element silicon was added to the aluminum melt with a nominal composition. The influence of silicon on the aluminizing and following oxidation process was investigated. With the addition of silicon into the aluminum melt, the coating became thinner and more homogeneous. The effort of the silicon on the oxidation behavior was observed as well concerning the suppression of porous band and cracks. (author)

  6. Organic fluid permeation through fluoropolymer membranes

    Science.gov (United States)

    Nemser, Stuart M.; Kosaraju, Praveen; Bowser, John

    2015-07-14

    Separation of the components of liquid mixtures is achieved by contacting a liquid mixture with a nonporous membrane having a fluoropolymer selectively permeable layer and imposing a pressure gradient across the membrane from feed side to permeate side. Unusually high transmembrane flux is obtained when the membrane is subjected to one or more process conditions prior to separation. These include (a) leaving some residual amount of membrane casting solvent in the membrane, and (b) contacting the membrane with a component of the mixture to be separated for a duration effective to saturate the membrane with the component.

  7. Phase Change Permeation Technology For Environmental Control Life Support Systems

    Science.gov (United States)

    Wheeler, Raymond M.

    2014-01-01

    Use of a phase change permeation membrane (Dutyion [Trademark]) to passively and selectively mobilize water in microgravity to enable improved water recovery from urine/brine for Environment Control and Life Support Systems (ECLSS) and water delivery to plans for potential use in microgravity.

  8. Diclofenac Salts, VIII. Effect of the Counterions on the Permeation through Porcine Membrane from Aqueous Saturated Solutions

    Science.gov (United States)

    Fini, Adamo; Bassini, Glenda; Monastero, Annamaria; Cavallari, Cristina

    2012-01-01

    The following bases: monoethylamine (EtA), diethylamine (DEtA), triethylamine (TEtA), monoethanolamine (MEA), diethanolamine (DEA), triethanolamine (TEA), pyrrolidine (Py), piperidine (Pp), morpholine (M), piperazine (Pz) and their N-2-hydroxyethyl (HE) analogs were employed to prepare 14 diclofenac salts. The salts were re-crystallized from water in order to obtain forms that are stable in the presence of water. Vertical Franz-type cells with a diffusional surface area of 9.62 cm2 were used to study the permeation of these diclofenac salts from their saturated solutions through an internal pig ear membrane. The receptor compartments of the cells contained 100 mL of phosphate buffer (pH 7.4); a saturated solution (5 mL) of each salt was placed in the donor compartment, thermostated at 37 °C. Aliquots were withdrawn at predetermined time intervals over 8 h and then immediately analyzed by HPLC. Fluxes were determined by plotting the permeated amount, normalized for the membrane surface area versus time. Permeation coefficients were obtained dividing the flux values J by the concentration of the releasing phase—that is, water solubility of each salt. Experimental results show that fluxes could be measured when diclofenac salts with aliphatic amines are released from a saturated aqueous solution. Different chemical species (acid, anion, ion pairs) contribute to permeation of the anti-inflammatory agent even though ion-pairs could be hypothesized to operate to a greater extent. Permeation coefficients were found higher when the counterion contains a ring; while hydroxy groups alone do not appear to play an important role, the ring could sustain permeation, disrupting the organized domains of the membrane. PMID:24300300

  9. Skin penetration and tissue permeation after topical administration of diclofenac.

    Science.gov (United States)

    Hagen, Martina; Baker, Mark

    2017-09-01

    Topical delivery of drugs is an alternative to oral administration, often with similar efficacy but potentially a more favorable tolerability profile. However, topical formulations need to be able to penetrate the skin and permeate to the target areas in quantities sufficient to exert a therapeutic effect. Many factors can affect this process, including the physicochemical properties of the drug, the formulation used, and the site and mode of application. It is believed that measurement of drug concentrations at the sites of action may be an indicator of their likely efficacy. This review addresses these issues, with reference to topically administered diclofenac in osteoarthritis. Articles relevant to this review were identified after a systematic search of Medline and Embase, using the key words "diclofenac", "topical administration" and "osteoarthritis" in the search strategy. The sparse data available indicate that topical diclofenac can penetrate and permeate to deeper tissues, with a lower plasma to tissue ratio than oral diclofenac. The tissue diclofenac levels after topical delivery are sustained over time (at least several hours). However, there is not enough data to establish how diclofenac levels in the joint compare with IC 50 levels (50% of the maximum inhibition of prostaglandin synthesis) established following oral administration. After topical application, diclofenac can penetrate the skin and permeate to deeper tissues, where it reaches a concentration that appears to be sufficient to exert a therapeutic effect. More robust methods are required for in vivo characterization to better estimate the clinical efficacy of topically applied drugs.

  10. Development of a hydrogen permeation sensor for future tritium applications

    Energy Technology Data Exchange (ETDEWEB)

    Llivina, L.; Colominas, S.; Abellà, J., E-mail: sergi.colominas@iqs.es

    2014-10-15

    Highlights: • Designing and testing of a hydrogen permeation sensor. • Palladium and α-iron have been used as a hydrogen permeation materials in the sensor. • The experiments performed using both membranes showed that the operation of the sensors in the equilibrium mode required at least several hours to reach the hydrogen equilibrium pressure. - Abstract: Tritium monitoring in lithium–lead eutectic is of great importance for the performance of liquid blankets in fusion reactors. In addition, tritium measurements will be required in order to proof tritium self-sufficiency in liquid metal breeding systems. On-line hydrogen (isotopes) sensors must be design and tested in order to accomplish these goals. In this work, an experimental set up was designed in order to test the permeation hydrogen sensors at 500 °C. This experimental set-up allowed working with controlled environments (different hydrogen partial pressures) and the temperature was measured using a thermocouple connected to a temperature controller that regulated an electrical heater. In a first set of experiments, a hydrogen sensor was constructed using an α-iron capsule as an active hydrogen area. The sensor was mounted and tested in the experimental set up. In a second set of experiments the α-iron capsule was replaced by a welded thin palladium disk in order to minimize the death volume. The experiments performed using both membranes (α-iron and palladium) showed that the operation of the sensors in the equilibrium mode required at least several hours to reach the hydrogen equilibrium pressure.

  11. Hydrogen permeation properties of Pd-coated V89.8Cr 10Y0.2 alloy membrane using WGS reaction gases

    KAUST Repository

    Jeon, Sungil

    2013-05-01

    The influence of co-existing gases on the hydrogen permeation was studied through a Pd-coated V89.8Cr10Y0.2 alloy membrane. Preliminary hydrogen permeation experiments have been confirmed that hydrogen flux was 6.26 ml/min/cm2 for a Pd-coated V 89.8Cr10Y0.2 alloy membrane (thick: 0.5 mm) using pure hydrogen as feed gas. Also, the hydrogen permeation flux decreased with decrease of hydrogen partial pressure at constant pressure when H 2/CO2 and H2/CO2/H2S mixture applied as feed gas respectively and permeation fluxes were satisfied with Sievert\\'s law in different feed conditions. It was found from XRD and SEM results after permeation test that the Pd-coated V89.8Cr 10Y0.2 alloy membrane had good stability and durability for various mixture feeding conditions. Copyright © 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.

  12. Hydrogen permeation properties of Pd-coated V89.8Cr 10Y0.2 alloy membrane using WGS reaction gases

    KAUST Repository

    Jeon, Sungil; Park, Junghoon

    2013-01-01

    The influence of co-existing gases on the hydrogen permeation was studied through a Pd-coated V89.8Cr10Y0.2 alloy membrane. Preliminary hydrogen permeation experiments have been confirmed that hydrogen flux was 6.26 ml/min/cm2 for a Pd-coated V 89.8Cr10Y0.2 alloy membrane (thick: 0.5 mm) using pure hydrogen as feed gas. Also, the hydrogen permeation flux decreased with decrease of hydrogen partial pressure at constant pressure when H 2/CO2 and H2/CO2/H2S mixture applied as feed gas respectively and permeation fluxes were satisfied with Sievert's law in different feed conditions. It was found from XRD and SEM results after permeation test that the Pd-coated V89.8Cr 10Y0.2 alloy membrane had good stability and durability for various mixture feeding conditions. Copyright © 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.

  13. Investigating the sonophoresis effect on the permeation of diclofenac sodium using 3D skin equivalent.

    Science.gov (United States)

    Aldwaikat, Mai; Alarjah, Mohammed

    2015-01-01

    Ultrasound temporally increases skin permeability by altering stratum corneum SC function (sonophoresis). The objective of this study was to evaluate the effect of variable ultrasound conditions on the permeation of diclofenac sodium DS with range of physicochemical properties through EpiDerm™. Permeation studies were carried out in vitro using Franz diffusion cell. HPLC method was used for the determination of the concentration of diclofenac sodium in receiving compartment. Parameters like ultrasound frequency, application time, amplitude, and mode of sonication and distance of ultrasound horn from skin were investigated, and the conditions where the maximum enhancement rate obtained were determined. Application of ultrasound enhanced permeation of diclofenac sodium across EpiDerm™ by fivefolds. The most effective enhancing parameters were power sonication of 20kHz frequency, 20% amplitude at continuous mode for 5min. Copyright © 2014. Published by Elsevier B.V.

  14. Effect of phonophoresis on skin permeation of commercial anti-inflammatory gels: sodium diclofenac and ketoprofen.

    Science.gov (United States)

    Souza, Jaqueline; Meira, Alianise; Volpato, Nadia Maria; Mayorga, Paulo; Gottfried, Carmem

    2013-09-01

    This study evaluated the use of ultrasound in combination with the commercial anti-inflammatory drugs ketoprofen and sodium diclofenac, according to the parameters used in physiotherapy. Ketoprofen and sodium diclofenac were used in the Franz diffusion cell model adapted to an ultrasound transducer in three conditions: no ultrasound, one application of ultrasound and two applications of ultrasound. High-performance liquid chromatography was used to quantify the total amount of drug permeating skin per unit area, as well as flux and latency. The results showed that for ketoprofen, the amount of drug permeating skin and flux increased with two ultrasound applications. Permeation of sodium diclofenac decreased in the presence of ultrasound. Ultrasound parameters and drug properties must be considered in the use of phonophoresis. Copyright © 2013 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.

  15. Peculiarities of hydrogen permeation through Zr–1%Nb alloy and evaluation of terminal solid solubility

    Energy Technology Data Exchange (ETDEWEB)

    Denisov, E.A.; Kompaniets, M.V.; Kompaniets, T.N., E-mail: tkompaniets@spbu.ru; Bobkova, I.S.

    2016-04-15

    Hydrogen permeation through Zr–1%Nb alloy was studied at the temperature below the temperature of α-β transition. Analysis of the transient permeation curves from a closed volume in a surface limited regime allowed to determine total and mobile hydrogen concentrations. At the mobile hydrogen concentration of 4.3 at% a part of the absorbed hydrogen is cut out of permeation process. Increase of the mobile hydrogen concentration in α-phase of Zr–1%Nb alloy is ceasing at the concentration of (5.5 ± 0.3) at%, which is the maximum possible concentration of the mobile hydrogen in α-phase of the studied alloy. From this moment on all absorbed hydrogen is spent on hydride formation. The obtained results are compared with those obtained by means of traditional techniques for terminal solid solubility determination.

  16. Investigation of aluminised steel as a barrier to tritium using accelerator-based and hydrogen permeation techniques

    Energy Technology Data Exchange (ETDEWEB)

    Sokhi, R S; Forcey, K S; Ross, D K; Earwaker, L G [Birmingham Univ. (UK). School of Physics and Space Research

    1989-04-01

    Aluminised steel has been proposed as a barrier to tritium permeation in fusion reactors. The properties of these materials as tritium barriers have been studied using accelerator-based techniques and hydrogen permeation methods. The aluminide layers has been characterised by Rutherford backscattering spectroscopy (RBS) and nuclear reaction analysis (NRA) techniques using the 3 MV Dynamitron accelerator based at the School of Physics and Space Research Radiation Centre. The effectiveness of the aluminide layer as a tritium barrier has been measured directly by a conventional permeation apparatus over a range of temperatures. The effect of high temperatures on the structure of the aluminide layer has been examined. Any correlation between the composition of the layer and its effectiveness as a tritium barrier is also discussed. (orig.).

  17. Deuterium permeation of amorphous alumina coating on 316L prepared by MOCVD

    Science.gov (United States)

    Li, Shuai; He, Di; Liu, Xiaopeng; Wang, Shumao; Jiang, Lijun

    2012-01-01

    The deuterium permeation behavior of the alumina coating on 316L stainless steel prepared by metal organic chemical vapor deposition (MOCVD) was investigated. The alumina coating was also characterized by X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and scanning electron microscope (SEM). It was found that the as-prepared coating consisted of amorphous alumina. This alumina coating had a dense, crack-free and homogeneous morphology. Although the alumina coating was amorphous, effective suppression of deuterium permeation was demonstrated. The deuterium permeability of the alumina coating was 51-60 times less than that of the 316L stainless steel and 153-335 times less than that of the referred low activation martensitic steels at 860-960 K.

  18. Oxygen- and hydrogen-permeation measurements on-mixed conducting SrFeCo{sub 0.5}O{sub y} ceramic membrane material

    Energy Technology Data Exchange (ETDEWEB)

    Serra, E.; Casagrande, E.; La Barbera, A. [ENEA UTS MAT, CR Casaccia, 00060 S.M. di Galeria, Roma (Italy); Alvisi, M. [ENEA UTS MAT, CR Brindisi, 72100 Brindisi (Italy); Bezzi, G.; Mingazzini, C. [ENEA UTS MAT, CR Faenza, 48018 Faenza (Italy)

    2008-02-15

    The SrFeCo{sub 0.5}O{sub y} system combines high electronic/ionic conductivity with appreciable oxygen permeability at elevated temperatures. This system has potential use in high-temperature electrochemical applications such as solid oxide fuel cells, batteries, sensors, and oxygen separation membranes. Dense ceramic membranes of SrFeCo{sub 0.5}O{sub y} are prepared by pressing a ceramic powder prepared by using a sol-gel combustion technique. Oxygen and hydrogen permeation at high temperature on this material are studied. Measurements are conducted using a time-dependent permeation method at the temperature in the range of 1073-1273 K with oxygen- and hydrogen-driving pressures in the range (3 x 10{sup 2})-(1 x 10{sup 5}) Pa (300-1000 mbar). The maximum oxygen-permeated flux at 1273 K is 6.5 x 10{sup -3} mol m{sup -2} s{sup -1}. The activation energies for the O{sub 2}-permeation fluxes and diffusivities are 240 and 194 kJ/mol, respectively. Due to the high fragility, the high temperature for the measurements and the high oxygen permeation through such material, a special membrane holder, and compression sealing system have been designed and realized for the permeation apparatus. (author)

  19. Gas Permeation Processes in Biogas Upgrading: A Short Review

    Czech Academy of Sciences Publication Activity Database

    Kárászová, Magda; Sedláková, Zuzana; Izák, Pavel

    2015-01-01

    Roč. 69, č. 10 (2015), s. 1277-1283 ISSN 0366-6352 R&D Projects: GA MŠk(CZ) LD14094; GA MŠk LH14006; GA ČR GA14-12695S Institutional support: RVO:67985858 Keywords : biogas upgrading * memranes * gas permeation Subject RIV: CI - Industrial Chemistry, Chemical Engineering Impact factor: 1.326, year: 2015

  20. Analysis of skin permeation-enhancing mechanism of iontophoresis using hydrodynamic pore theory.

    Science.gov (United States)

    Manabe, E; Numajiri, S; Sugibayashi, K; Morimoto, Y

    2000-05-15

    The effects of constant DC iontophoresis (0-1.5 mA/0.966 cm(2)) on the permeation of three hydrophilic compounds, antipyrine (ANP, M.W. 188.23), sucrose (SR, M.W. 342.30) and 1-kestose (KT, M.W. 506.73), through excised hairless rat skin were evaluated using hydrodynamic pore theory. The electro-osmotic flow caused by iontophoresis was measured using deuterium oxide (D(2)O). The penetration-enhancing mechanism of iontophoresis was found to increase solvent flow through electro-osmosis and pore enlargement and/or new pore production in the skin barrier, together with enhancement of electrochemical potential difference across the skin. These effects were closely related to the strength of the current applied. The electro-osmotic flow of D(2)O (J(D(2)O)) greatly enhanced the skin permeation clearance of all hydrophilic penetrants (CL(drug)). Pore production was classified into reversible and irreversible processes, which resulted from lower (0-0.5 mA/0.966 cm(2)) and higher (0.5-1. 5 mA/0.966 cm(2)) currents, respectively. Thus, the enhancing effects of iontophoresis on skin permeation of nonionic hydrophilic compounds can be explained by increase in pore size and higher solvent flow.

  1. Effect of emulsification on the skin permeation and UV protection of catechin.

    Science.gov (United States)

    Yoshino, Sachie; Mitoma, Tomoaki; Tsuruta, Keiko; Todo, Hiroaki; Sugibayashi, Kenji

    2014-06-01

    An anti-aging effect may be obtained by skin application of tea catechins (Camellia sinensis) since they have high ultraviolet (UV)-protection activity. In this study, the skin permeation of catechin (C), epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECg) and epigallocatechin gallate (EGCg) was determined and compared, and the effect of emulsification on the skin permeation of C was measured. The UV-protective effect of C was also determined. The in vitro skin permeability of each catechin derivative was determined using side-by-side diffusion of cells. The UV-protective effect of C was determined by applying different concentrations of C to the solution or emulsion on a three-dimensional cultured human skin model or normal human epidermal keratinocytes with UV-irradiation. ECg and EGCg with gallate groups showed lower skin permeability than C, EC and EGC without gallate groups, suggesting that the skin permeability of catechin derivatives may be dependent on the existence of a gallate group. Interestingly, the skin permeation of C was increased by an o/w emulsification. In addition, the C emulsion showed a significantly higher UV-protective effect by C than that with its aqueous solution. These results suggest that the o/w emulsion of catechin derivatives is probably useful as a cosmetic formulation with anti-aging efficacy.

  2. Evaluation of a dynamic dissolution/permeation model

    DEFF Research Database (Denmark)

    Sironi, Daniel; Christensen, Mette; Rosenberg, Jörg

    2017-01-01

    -steady state). To this end, a model case was construed: compacts of pure crystalline hydrocortisone methanolate (HC·MeOH) of slow release rates were prepared, and their dissolution and permeation determined simultaneously in a side-by-side setup, separated by a biomimetic barrier (Permeapad...... dissolution rate and flux influenced each other. Interestingly, for all the dynamic scenarios, the incremental flux values obtained correlated nicely with the corresponding actual donor concentrations. Furthermore, donor depletion was tested using a HC solution. The dynamic interplay between decrease in donor...

  3. Measurement of blood-brain barrier permeation in rats during exposure to 2450-MHz microwaves

    International Nuclear Information System (INIS)

    Ward, T.R.; Elder, J.A.; Long, M.D.; Svendsgaard, D.

    1982-01-01

    Adult rats anesthesized with pentobarbital and injected intravenously with a mixture of [ 14 C] sucrose and [ 3 H] inulin were exposed for 30 min to an environment at an ambient temperature of 22, 30, or 40 degrees C, or were exposed at 22 degrees C to 2450-MHz CW microwave radiation at power densities of 0, 10, 20, or 30 mW/cm2. Following exposure, the brain was perfused and sectioned into eight regions, and the radioactivity in each region was counted. The data were analyzed by two methods. First, the data for each of the eight regions and for each of the two radioactive tracers were analyzed by regression analysis for a total of 16 analyses and Bonferroni's Inequality was applied to prevent false positive results from numerous analyses. By this conservative test, no statistically significant increase in permeation was found for either tracer in any brain region of rats exposed to microwaves. Second, a profile analysis was used for a general change in tracer uptake across all brain regions. Using this statistical method, a significant increase in permeation was found for sucrose but not for inulin. A correction factor was then derived from the warm-air experiments to correct for the increase in permeation of the brain associated with change in body temperature. This correction factor was applied to the data for the irradiated animals. After correcting the data for thermal effects of the microwave radiation, no significant increase in permeation was found

  4. Gastrointestinal behavior of nano- and microsized fenofibrate: In vivo evaluation in man and in vitro simulation by assessment of the permeation potential.

    Science.gov (United States)

    Hens, Bart; Brouwers, Joachim; Corsetti, Maura; Augustijns, Patrick

    2015-09-18

    The purpose of this study was (i) to evaluate the gastrointestinal behavior of micro- and nanosized fenofibrate in humans and (ii) to develop a simple yet qualitatively predictive in vitro setup that simulates the observed absorption-determining factors. Commercially available micro- and nanoparticles of fenofibrate (Lipanthyl® and Lipanthylnano®, respectively) were administered orally to five healthy volunteers in fasting and postprandial conditions. Intraluminal and systemic drug concentrations were determined as reference data for the development of a predictive in vitro setup. To capture the observed solubility/permeability interplay, in vitro dissolution testing was performed in the presence of a permeation bag with sink conditions. In fasting conditions, intake of nanosized fenofibrate generated increased duodenal concentrations compared to microsized fenofibrate, which was reflected in an improved systemic exposure. In postprandial conditions, duodenal concentrations were greatly enhanced for both formulations, however without an accompanying increase in systemic exposure. It appeared that micellar encapsulation of the highly lipohilic fenofibrate limited its potential to permeate from fed state intestinal fluids. To capture these in vivo observations in an in vitro setup, classic dissolution testing was combined with permeation assessment into a permeation bag with sink conditions. In case of fasting conditions, the dissolution/permeation approach allowed for an improved discriminative power between micro- and nanosized fenofibrate by better simulating the dynamic interplay of dissolution and absorption. In case of postprandial conditions, the observed solubility-permeability interplay could be simulated using the dissolution/permeation approach in combination with biorelevant media (FeSSGFFortimel and FeSSIF-V2) to mimic micellar entrapment and reduced permeation potential of fenofibrate. For the first time, reduced permeation of a lipophilic drug

  5. Needs and gaps in the development of aluminum-based corrosion and T-permeation barriers for DEMO blankets

    Energy Technology Data Exchange (ETDEWEB)

    Wulf, Sven-Erik, E-mail: sven-erik.wulf@kit.edu; Krauss, Wolfgang; Konys, Jürgen

    2015-10-15

    Highlights: • New processes for barriers based on electroplating introduced in the last years. • New processes ECA and ECX able to overcome former fabrication problems. • Scales by ECA showed long-term compatibility in flowing Pb–Li (>12,000 h). • Further fusion relevant characterization and optimization of scales is required. • Qualification of T-permeation properties is urgently needed. - Abstract: Low-activation-ferritic–martensitic (RAFM) steels are candidates for structural materials in different blanket designs foreseen for DEMO and partly for TBM's tested in ITER. In all designs the liquid breeder Pb–15.7Li is in direct contact with the structural material, and thus two major topics – corrosion and T-permeation – influence the reliable, safe and economical application of such combination of breeder and structural material. As bare RAFM steels exhibit high corrosion rates of up to 400 μm/h in flowing Pb–15.7Li, Al-based coatings made by different coating processes were developed during the last 15 years and showed promising results in protecting RAFM steels from corrosion and T-permeation reduction. Especially barriers made by HDA, and electroplating (ECA, ECX), proved their ability to protect Eurofer against corrosion in flowing Pb–15.7Li. However, available T-permeation data for coated RAFM steels are rare and partly ambiguous for these coatings. This paper summarizes the state-of-the-art of aluminum-based barrier development and points out gaps and needs in future scale characterization and T-permeation barrier development. Additionally, necessary qualification steps on the path toward a reliable fabrication route are presented that is required to produce aluminum-based corrosion and T-permeation barriers on RAFM steels for blanket applications in future fusion reactors like DEMO.

  6. The influence of pH on the in vitro permeation of rhodium through human skin.

    Science.gov (United States)

    Jansen Van Rensburg, Sané; Franken, Anja; Du Plessis, Jeanetta; Du Plessis, Johannes Lodewykus

    2017-06-01

    Workers in precious metals refineries are at risk of exposure to salt compounds of the platinum group metals through inhalation, as well as through the skin. Rhodium salt permeation through the skin has previously been proven using rhodium trichloride (RhCl 3 ) dissolved in synthetic sweat at a pH of 6.5. However, the skin surface pH of refinery workers may be lower than 6.5. The aim of this study was to investigate the influence of pH 6.5 and 4.5 on the in vitro permeation of rhodium through intact Caucasian skin using Franz diffusion cells. A concentration of 0.3 mg mL -1 rhodium was used and analyses were performed using inductively coupled plasma mass spectrometry and inductively coupled plasma optical emission spectrometry. Results indicated a cumulative increase in permeation over 24 h. Rhodium permeation after 12 h was significantly greater at pH 4.5 (1.56 ± 0.24 ng cm -2 ) than at 6.5 (0.85 ± 0.13 ng cm -2 ; p = 0.02). At both pH levels, there was a highly significant difference ( p rhodium remaining in the skin (1428.68 ± 224.67 ng cm -2 at pH 4.5 and 1029.90 ± 115.96 ng cm -2 at pH 6.5) and the mass that diffused through (0.88 ± 0.17 ng cm -2 at pH 4.5 and 0.62 ± 0.10 ng cm -2 at pH 6.5). From these findings, it is evident that an acidic working environment or low skin surface pH may enhance permeation of rhodium salts, contributing to sensitization and adverse health effects.

  7. Performance Tests of a Permeation Sensor for Test Blanket Modules Using Liquid Metal

    International Nuclear Information System (INIS)

    Choi, B. G.; Lee, D. W.; Lee, E. H.; Yoon, J. S.; Kim, S. K.; Shin, K. I.; Jin, H. G.

    2013-01-01

    The tritium extraction from a breeder is one of the key technologies and its methods have been investigated. For developing the tritium extraction methods and evaluating the amount of tritium in the system, a reliable and correct sensor is required to measure the hydrogen concentration in liquid metal breeder. There are several researches for developing the sensors in the ITER participants and especially, EU has developed the permeation sensors trying to selecting materials with low Serviette's constant (solubility) and high hydrogen diffusivity coefficient. However, EU's response time is still too long time about tens of minutes to measure the tritium concentration in the online system. We have been performing the preliminary tests with designed and fabricated sensors to solve the late response of sensor. However, we could not continue the tests because of the membrane's oxidation (pure Fe) and the difficulty of welding nonferrous metals. In present study, a permeation sensor made of vacuum flanges with a porous plate inside is proposed not only to eliminate the difficulty of the fabrication but to optimize the performance of sensor. The permeation sensor to measure the hydrogen isotopes in liquid metal breeder has been proposed and evaluated to overcome the limitation of a long response time for various shapes and materials. We found that the previous sensors have limitation; the oxidation problems (pure Fe) and the difficulty in welding (nonferrous metals). Therefore we proposed a permeation sensor with the vacuum flanges filled with porous disks to eliminate the problems. By using the CF flanges, the problem caused by welding is removed. But the permeable response time of sensors took a long time to reach the pressure equivalent

  8. Effect of feed flow pattern on the distribution of permeate fluxes in desalination by direct contact membrane distillation

    KAUST Repository

    Soukane, Sofiane

    2017-05-31

    The current study aims to highlight the effect of flow pattern on the variations of permeate fluxes over the membrane surface during desalination in a direct contact membrane distillation (DCMD) flat module. To do so, a three dimensional (3D) Computational Fluid Dynamics (CFD) model with embedded pore scale calculations is implemented to predict flow, heat and mass transfer in the DCMD module. Model validation is carried out in terms of average permeate fluxes with experimental data of seawater desalination using two commercially available PTFE membranes. Average permeate fluxes agree within 6% and less with experimental values without fitting parameters. Simulation results show that the distribution of permeate fluxes and seawater salinity over the membrane surface are strongly dependent on momentum and heat transport and that temperature and concentration polarization follow closely the flow distribution. The analysis reveals a drastic effect of recirculation loops and dead zones on module performance and recommendations to improve MD flat module design are drawn consequently.

  9. Measuring deuterium permeation through tungsten near room temperature under plasma loading using a getter layer and ion-beam based detection

    Directory of Open Access Journals (Sweden)

    Stefan Kapser

    2017-08-01

    Full Text Available A method to measure deuterium permeation through tungsten near room temperature under plasma loading is presented. The permeating deuterium is accumulated in a getter layer of zirconium, titanium or erbium, respectively, on the unexposed side of the sample. Subsequently, the amount of deuterium in the getter is measured ex-situ using nuclear reaction analysis. A cover layer system on the getter prevents direct loading of the getter with deuterium from the gas phase during plasma loading. In addition, it enables the distinction of deuterium in the getter and at the cover surface. The method appears promising to add additional permeation measurement capabilities to deuterium retention experiments, also in other plasma devices, without the need for a complex in-situ permeation measurement setup.

  10. Hydrogen isotope permeation in elastomeric materials

    International Nuclear Information System (INIS)

    Steinmeyer, R.H.; Braun, J.D.

    1976-01-01

    The permeabilities of elastomeric and polymeric materials to hydrogen isotopes were measured at room temperature. The technique for measuring permeation rates is based on the following constant-volume method: a fixed pressure of gas is applied to one side of the specimen to be studied and the permeability constant is determined from the observed rate of pressure increase in an initially evacuated volume on the other side of the specimen. Permeability constants for hydrogen, deuterium, and tritium were measured for Mylar, Teflon, Kapton, Saran, Buna-N, and latex rubber. Results were compared with literature values for hydrogen and deuterium where available and showed excellent agreement

  11. Lyophilized phytosomal nanocarriers as platforms for enhanced diosmin delivery: optimization and ex vivo permeation

    Directory of Open Access Journals (Sweden)

    Freag MS

    2013-07-01

    Full Text Available May S Freag, Yosra SR Elnaggar, Ossama Y AbdallahDepartment of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria, EgyptAbstract: Diosmin (DSN is an outstanding phlebotonic flavonoid with a tolerable potential for the treatment of colon and hepatocellular carcinoma. Being highly insoluble, DSN bioavailability suffers from high inter-subject variation due to variable degrees of permeation. This work endeavored to develop novel DSN loaded phytosomes in order to improve drug dissolution and intestinal permeability. Three preparation methods (solvent evaporation, salting out, and lyophilization were compared. Nanocarrier optimization encompassed different soybean phospholipid (SPC types, different solvents, and different DSN:SPC molar ratios (1:1, 1:2, and 1:4. In vitro appraisal encompassed differential scanning calorimetry, infrared spectroscopy, particle size, zeta potential, polydispersity index, transmission electron microscopy, drug content, and in vitro stability. Comparative dissolution studies were performed under sink versus non-sink conditions. Ex vivo intestinal permeation studies were performed on rats utilizing noneverted sac technique and high-performance liquid chromatography analysis. The results revealed lyophilization as the optimum preparation technique using SPC and solvent mixture (Dimethyl sulphoxide:t-butylalchol in a 1:2 ratio. Complex formation was contended by differential scanning calorimetry and infrared data. Optimal lyophilized phytosomal nanocarriers (LPNs exhibited the lowest particle size (316 nm, adequate zeta-potential (−27 mV, and good in vitro stability. Well formed, discrete vesicles were revealed by transmission electron microscopy, drug content, and in vitro stability. Comparative dissolution studies were performed. LPNs demonstrated significant enhancement in DSN dissolution compared to crude drug, physical mixture, and generic and brand DSN products. Permeation studies revealed 80% DSN

  12. Hydrogen diffusion, dissolution and permeation of nonmetallic solids

    International Nuclear Information System (INIS)

    Elleman, T.S.; Rao, D.; Verghese, K.; Zumwalt, L.

    1979-01-01

    A review of hydrogen diffusion, dissolution and permeation in metal oxides, carbides, nitrides, halides and hydrides is presented. Results are organized by compound and an effort has been made to resolve differences between measured results where wide disparities exist. The document has been prepared to provide needed data for the development of fusion reactor blankets but the results should be generally useful in technologies that involve interactions between hydrogen and non-metals

  13. In vitro skin permeation and decontamination of the organophosphorus pesticide paraoxon under various physical conditions--evidence for a wash-in effect.

    Science.gov (United States)

    Misik, Jan; Pavlikova, Ruzena; Josse, Denis; Cabal, Jiri; Kuca, Kamil

    2012-09-01

    Misuse of various chemicals, such as chemical warfare agents, industrial chemicals or pesticides during warfare or terrorists attacks requires adequate protection. Thus, development and evaluation of novel decontamination dispositives and techniques are needed. In this study, in vitro permeation and decontamination of a potentially hazardous compound paraoxon, an active metabolite of organophosphorus pesticide parathion, was investigated. Skin permeation and decontamination experiments were carried out in modified Franz diffusion cells. Pig skin was used as a human skin model. Commercially produced detergent-based washing solutions FloraFree(™) and ArgosTM were used as decontamination means. The experiments were done under "warm", "cold", "dry" and "wet" skin conditions in order to determine an effect of various physical conditions on skin permeation of paraoxon and on a subsequent decontamination process. There was no significant difference in skin permeation of paraoxon under warm, cold and dry conditions, whereas wet conditions provided significantly higher permeation rates. In the selected conditions, decontamination treatments performed 1 h after a skin exposure did not decrease the agent volume that permeated through the skin. An exception were wet skin conditions with non-significant decontamination efficacy 18 and 28% for the FloraFree(™) and Argos(™) treatment, respectively. In contrast, the skin permeation of paraoxon under warm, cold and dry conditions increased up to 60-290% following decontamination compared to non-decontaminated controls. This has previously been described as a skin wash-in effect.

  14. Mineralogical and chemical-physical effects of hydrocarbon permeation in composite liners and cut-off walls. Final report; Mineralogische und chemisch-physikalische Auswirkungen der Permeation von Kohlenwasserstoffen in Kombinationsdichtungen und -dichtwaenden. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Kalbe, U; Berger, W; Mueller, W; Brune, M; Eckardt, J; Tatzky-Gerth, R; Ache, W; Goebbels, J [Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin (Germany); Breu, J; Kerzdoerfer, H [Regensburg Univ. (Germany). Inst. fuer Anorganische Chemie

    2000-05-31

    Composite liner systems (HDPE geomembrane and compacted mineral liner) are used in Germany in landfills and for the lining of contaminated sites according to the technical regulations. It is expected that these lining systems provide a highly efficient and reliable technical barrier for the long-term groundwater protection. To support these expectations and assess the performance of the liner system even under extreme conditions, various composite liner systems were exposed to a mixture of 9 liquid hydrocarbons and their permeation behaviour was studied in permeation cells over 12 years. The cells were now dismantled and changes in the liner materials were carefully measured and controlled. The following issues were pursued in the research project: - effect of long-term hydrocarbon permeation and immersion on the properties of the geomembrane, - determination of the vertical distribution of organic contaminants in the mineral liner, - changes in the mineralogical, micromorphological and soil mechanical properties of the mineral liner brought about by the contaminant mixture, - investigation of the influence of microbial activity on the mineral layer, - modelling of the pollutant transport in the composite liner system. Neither geomembrane nor most of the tested mineral liners exhibited significant changes. Hydrocarbon permeation was proved to have been substantially suppressed by the composite liner. (orig.) [German] Zur Sicherung von Deponien und Altlasten mit dem Ziel eines langfristig wirksamen Grundwasserschutzes werden seit Mitte der 80er Jahre Kombinationsdichtungen (Verbund aus Kunststoffdichtungsbahn und mineralischer Dichtschichten) eingesetzt. Um deren Langzeitbestaendigkeit auch unter extremen Bedingungen bewerten zu koennen, wurden Permeationsmesszellen, welche die Verhaeltnisse in der Deponie nachstellen und ueber einen Zeitraum von 12 Jahren mit einem Mehrkomponentengemisch konzentrierter organischer Verbindungen beaufschlagt worden waren, zerlegt und

  15. Ionic Selectivity and Permeation Properties of Human PIEZO1 Channels.

    Directory of Open Access Journals (Sweden)

    Radhakrishnan Gnanasambandam

    Full Text Available Members of the eukaryotic PIEZO family (the human orthologs are noted hPIEZO1 and hPIEZO2 form cation-selective mechanically-gated channels. We characterized the selectivity of human PIEZO1 (hPIEZO1 for alkali ions: K+, Na+, Cs+ and Li+; organic cations: TMA and TEA, and divalents: Ba2+, Ca2+, Mg2+ and Mn2+. All monovalent ions permeated the channel. At a membrane potential of -100 mV, Cs+, Na+ and K+ had chord conductances in the range of 35-55 pS with the exception of Li+, which had a significantly lower conductance of ~ 23 pS. The divalents decreased the single-channel permeability of K+, presumably because the divalents permeated slowly and occupied the open channel for a significant fraction of the time. In cell-attached mode, 90 mM extracellular divalents had a conductance for inward currents carried by the divalents of: 25 pS for Ba2+ and 15 pS for Ca2+ at -80 mV and 10 pS for Mg2+ at -50 mV. The organic cations, TMA and TEA, permeated slowly and attenuated K+ currents much like the divalents. As expected, the channel K+ conductance increased with K+ concentration saturating at ~ 45 pS and the KD of K+ for the channel was 32 mM. Pure divalent ion currents were of lower amplitude than those with alkali ions and the channel opening rate was lower in the presence of divalents than in the presence of monovalents. Exposing cells to the actin disrupting reagent cytochalasin D increased the frequency of openings in cell-attached patches probably by reducing mechanoprotection.

  16. Design of a permeator against vacuum for tritium extraction from eutectic lithium-lead in a DCLL DEMO

    Energy Technology Data Exchange (ETDEWEB)

    Garcinuño, Belit, E-mail: belit.garcinuno@ciemat.es [CIEMAT-LNF (Laboratorio Nacional de Fusión), Madrid (Spain); Rapisarda, David [CIEMAT-LNF (Laboratorio Nacional de Fusión), Madrid (Spain); Fernández, Iván [Fundación & Departamento de Ingeniería Energética, UNED, Madrid (Spain); CIEMAT-LNF (Laboratorio Nacional de Fusión), Madrid (Spain); Moreno, Carlos; Palermo, Iole; Ibarra, Ángel [CIEMAT-LNF (Laboratorio Nacional de Fusión), Madrid (Spain)

    2017-04-15

    Highlights: • A conceptual design of a Permeator Against Vacuum is presented. • The efficiency is dependent on geometry and Tritium transport. • The use of different membrane materials is discussed. • A squared PAV with alternated PbLi flowing and vacuum flat ducts is designed. • 80% efficiency of Tritium extraction is accomplished under DCLL-BB requirements. - Abstract: One of the most important issues in future fusion power plants is the extraction of tritium generated in the breeders in order to achieve self-sufficiency. When the breeder is a liquid metal one of the most promising techniques is the Permeation Against Vacuum, whose principle is based on tritium diffusion through a permeable membrane in contact with the liquid metal carrier and its further extraction by a vacuum pump. A conceptual design of permeator has been developed, taking into account the features of a DEMO reactor with a Dual Coolant Lithium Lead (DCLL) breeder blanket. The study is based on the analysis of different membranes and geometries aiming at the overall efficiency (extraction capability) of the device, as well as its compatibility with the breeder material. The permeator is based on a rectangular section multi-channel distribution where the liquid metal channels and vacuum channels are alternated in order to maximize the contact area and therefore to promote tritium transport from the bulk to the walls. The resulting permeator design has an excellent estimated extraction efficiency, of 80%, in a relatively compact device.

  17. Toward enhanced hydrogen generation from water using oxygen permeating LCF membranes

    KAUST Repository

    Wu, Xiao-Yu; Chang, Le; Uddi, Mruthunjaya; Kirchen, Patrick; Ghoniem, Ahmed F.

    2015-01-01

    and flow rates on the feed and sweep sides on the water thermolysis rate and oxygen flux. A single step reaction mechanism is proposed for surface reactions, and three-resistance permeation models are derived. Results show that water thermolysis

  18. Study of the cathodic depolarization theory with hydrogen permeation and the bacteria Desulfovibrio desulfuricans

    International Nuclear Information System (INIS)

    Romero, M. F. de; Duque, Z.; Rinco, O. T. de; Perez, O.; Araujo, I.

    2003-01-01

    A Desulfovibrio desulfuricans ssp. desulfuricans (SRB) was used to study the permeation of hydrogen, using a Devanatan and Stachurski cell and a palladium sheet. The aim was to evaluate cathodic depolarization as a Sulfate-Reducing Bacteria action mechanisms in Microbiologically Induced Corrosion. The permeation tests were run with and without cathodic polarization, using a sterile deaerated culture medium inoculated with 10% SRB concentrated at 10''8 cell/ml. the results indicate bacterial growth in the order of 10''9-10''10 cel/ml after 18 h both in the polarized and non-polarized, tests, indicating that SRB developed regardless of the surface polarized as a source of H''0, generating H 2 S as a product of the anaerobic respiration. It was also determined that, without cathodic polarization, the conditions are not enough to reduce the H* generated by the H 2 S dissociation (pd is not susceptible to corrosion at this condition). On the other hand, cathodic polarization increased the permeation current, which was associated with the maximum enzymatic activity phase of the bacteria. (Author) 8 refs

  19. Techniques for Large-Scale Bacterial Genome Manipulation and Characterization of the Mutants with Respect to In Silico Metabolic Reconstructions.

    Science.gov (United States)

    diCenzo, George C; Finan, Turlough M

    2018-01-01

    The rate at which all genes within a bacterial genome can be identified far exceeds the ability to characterize these genes. To assist in associating genes with cellular functions, a large-scale bacterial genome deletion approach can be employed to rapidly screen tens to thousands of genes for desired phenotypes. Here, we provide a detailed protocol for the generation of deletions of large segments of bacterial genomes that relies on the activity of a site-specific recombinase. In this procedure, two recombinase recognition target sequences are introduced into known positions of a bacterial genome through single cross-over plasmid integration. Subsequent expression of the site-specific recombinase mediates recombination between the two target sequences, resulting in the excision of the intervening region and its loss from the genome. We further illustrate how this deletion system can be readily adapted to function as a large-scale in vivo cloning procedure, in which the region excised from the genome is captured as a replicative plasmid. We next provide a procedure for the metabolic analysis of bacterial large-scale genome deletion mutants using the Biolog Phenotype MicroArray™ system. Finally, a pipeline is described, and a sample Matlab script is provided, for the integration of the obtained data with a draft metabolic reconstruction for the refinement of the reactions and gene-protein-reaction relationships in a metabolic reconstruction.

  20. Permeation of supercritical carbon dioxide through polymeric hollow fiber membranes

    NARCIS (Netherlands)

    Patil, V.E.; Broeke, van den L.J.P.; Vercauteren, F.F.; Keurentjes, J.T.F.

    2006-01-01

    Permeation of carbon dioxide was measured for two types of composite polymeric hollow fiber membranes for feed pressures up to 18 MPa at a temp. of 313 K. support membrane. The membranes consist of a polyamide copolymer (IPC) layer or a poly(vinyl alc.) (PVA) layer on top of a polyethersulfone

  1. The influence of corneocyte structure on the interpretation of permeation profiles of nanoparticles across skin

    Energy Technology Data Exchange (ETDEWEB)

    Pinheiro, T. [LFI, Instituto Tecnologico Nuclear, and Centro de Fisica Nuclear, Universidade Lisboa E.N. 10, 2685-953 Sacavem (Portugal)]. E-mail: murmur@itn.pt; Pallon, J. [Lund Institute of Technology, Physics Department, Lund University, Lund (Sweden)]. E-mail: Jan.Pallon@pixe.lth.se; Alves, L.C. [LFI, Instituto Tecnologico Nuclear, and Centro de Fisica Nuclear, Universidade Lisboa E.N. 10, 2685-953 Sacavem (Portugal)]. E-mail: lcalves@itn.pt; Verissimo, A. [LFI, Instituto Tecnologico Nuclear, and Centro de Fisica Nuclear, Universidade Lisboa E.N. 10, 2685-953 Sacavem (Portugal)]. E-mail: averissimo@vims.edu; Filipe, P. [Departamento Dermatologia, Hospital Sta. Maria, Lisbon (Portugal)]. E-mail: pfilipe@fm.ul.pt; Silva, J.N. [Departamento Dermatologia, Hospital Sta. Maria, Lisbon (Portugal)]. E-mail: maiasilva@fm.ul.pt; Silva, R. [Departamento Dermatologia, Hospital Sta. Maria, Lisbon (Portugal)]. E-mail: rpalminhas@netcabo.pt

    2007-07-15

    The permeability of skin to nanoparticles of titanium dioxide (TiO{sub 2}) used in sunscreens as a reflector of the UV wavelengths of sunlight, was examined using nuclear microscopy techniques. Special attention was given to the permeation characteristics of these nanoparticles across the outer layers of skin, the stratum corneum, in healthy and psoriatic skin condition. Aspects that may influence the interpretation of results such as sample preparation difficulties and skin condition were focused. Sample preparation can damage the integrity of the corneocyte layers inducing unwanted artefacts that may bias the evaluation of results. Irradiation conditions may also introduce distortions in the labile structures of human skin. Skin condition, such as loss of corneocyte cohesion occurring in psoriasis also influence the permeation profile of the nanoparticles. Weighing and accounting for these features in the examination of skin by nuclear microscopy is crucial to accurately assess the TiO{sub 2} nanoparticles permeation depth.

  2. Evaluation of γ-cyclodextrin effect on permeation of lipophilic drugs: application of cellophane/fused octanol membrane.

    Science.gov (United States)

    Muankaew, Chutimon; Jansook, Phatsawee; Loftsson, Thorsteinn

    2017-06-01

    According to the Biopharmaceutics Classification System, oral bioavailability of drugs is determined by their aqueous solubility and the ability of the dissolved drug molecules to permeate lipophilic biological membranes. Similarly topical bioavailability of ophthalmic drugs is determined by their solubility in the aqueous tear fluid and their ability to permeate the lipophilic cornea. Enabling pharmaceutical excipients such as cyclodextrins can have profound effect on the drug bioavailability. However, to fully appreciate such enabling excipients, the relationship between their effects and the physicochemical properties of the permeating drug needs to be known. In this study, the permeation enhancing effect of γ-cyclodextrin (γCD) on saturated drug solutions containing hydrocortisone (HC), irbesartan (IBS), or telmisartan (TEL) was evaluated using cellophane and fused cellulose-octanol membranes in a conventional Franz diffusion cell system. The flux (J), the flux ratio (J R ) and the apparent permeability coefficients (P app ) demonstrate that γCD increases drug permeability. However, its efficacy depends on the drug properties. Addition of γCD increased P app of HC (unionized) and IBS (partially ionized) through the dual membrane but decreased the P app of TEL (fully ionized) that displays low complexation efficacy. The dual cellophane-octanol membrane system was simple to use and gave reproducible results.

  3. Permeability, transport, and metabolism of solutes in Caco-2 cell monolayers: a theoretical study.

    Science.gov (United States)

    Sun, Huadong; Pang, K Sandy

    2008-01-01

    We explored the properties of a catenary model that includes the basolateral (B), apical (A), and cellular compartments via simulations under linear and nonlinear conditions to understand the asymmetric observations arising from transporters, enzymes, and permeability in Caco-2 cells. The efflux ratio (EfR; P(app,B-->A)/P(app,A-->B)), obtained from the effective permeability from the A-->B and B-->A direction under linear conditions, was unity for passively permeable drugs whose transport does not involve transporters; the value was unaffected by cellular binding or metabolism, but increased with apical efflux. Metabolism was asymmetric, showing lesser metabolite accrual for the B-->A than A-->B direction because of inherent differences in the volumes for A and B. Moreover, the net flux (total - passive permeation) due to saturable apical efflux, absorption, or metabolism showed nonconformity to simple Michaelis-Menten kinetics against C(D,0), the loading donor concentration. EfR values differed with saturable apical efflux and metabolism (>1), as well as apical absorption (EfRs transport and metabolic data in Caco-2 cells.

  4. Tritium inventory and permeation in liquid breeder blankets

    International Nuclear Information System (INIS)

    Reiter, F.

    1990-01-01

    This report reviews studies of the transport of hydrogen isotopes in the DEMO relevant water-cooled Pb-17Li blanket to be tested in NET and in a self-cooled blanket which uses Pb-17Li or Flibe as a liquid breeder material and V or Fe as a first wall material. The time dependences of tritium inventory and permeation in these blankets and of deuterium and tritium recycling in the self-cooled blanket are presented and discussed

  5. Preparation of Silica Nanoparticles Loaded with Nootropics and Their In Vivo Permeation through Blood-Brain Barrier

    OpenAIRE

    Jampilek, Josef; Zaruba, Kamil; Oravec, Michal; Kunes, Martin; Babula, Petr; Ulbrich, Pavel; Brezaniova, Ingrid; Opatrilova, Radka; Triska, Jan; Suchy, Pavel

    2015-01-01

    The blood-brain barrier prevents the passage of many drugs that target the central nervous system. This paper presents the preparation and characterization of silica-based nanocarriers loaded with piracetam, pentoxifylline, and pyridoxine (drugs from the class of nootropics), which are designed to enhance the permeation of the drugs from the circulatory system through the blood-brain barrier. Their permeation was compared with non-nanoparticle drug substances (bulk materials) by means of an i...

  6. Permeation studies of novel terbinafine formulations containing hydrophobins through human nails in vitro.

    Science.gov (United States)

    Vejnovic, Ivana; Huonder, Cornelia; Betz, Gabriele

    2010-09-15

    Existing treatments of onychomycosis are not satisfactory. Oral therapies have many side effects and topical formulations are not able to penetrate into the human nail plate and deliver therapeutical concentrations of active agent in situ. The purpose of the present study was to determine the amount of terbinafine, which permeates through the human nail plate, from liquid formulations containing enhancers, namely hydrophobins A-C in the concentration of 0.1% (w/v). The used reference solution contained 10% (w/v) of terbinafine in 60% (v/v) ethanol/water without enhancer. Permeability studies have been performed on cadaver nails using Franz diffusion cells modified to mount nail plates and filled with 60% (v/v) ethanol/water in the acceptor chamber. Terbinafine was quantitatively determined by HPLC. The amount of terbinafine remaining in the nail was extracted by 96% ethanol from pulverized nail material after permeation experiment and presented as percentage of the dry nail weight before the milling test. Permeability coefficient (PC) of terbinafine from reference solution was determined to be 1.52E-10 cm/s. Addition of hydrophobins improved PC in the range of 3E-10 to 2E-9 cm/s. Remaining terbinafine reservoir in the nail from reference solution was 0.83% (n=2). An increase of remaining terbinafine reservoir in the nail was observed in two out of three tested formulations containing hydrophobins compared to the reference. In all cases, known minimum inhibitory concentration of terbinafine for dermatophytes (0.003 microg/ml) has been exceeded in the acceptor chamber of the diffusion cells. All tested proteins (hydrophobins) facilitated terbinafine permeation after 10 days of permeation experiment, however one of them achieved an outstanding enhancement factor of 13.05 compared to the reference. Therefore, hydrophobins can be included in the list of potential enhancers for treatment of onychomycosis. Copyright 2010 Elsevier B.V. All rights reserved.

  7. Modeling the concentration-dependent permeation modes of the KcsA potassium ion channel.

    Science.gov (United States)

    Nelson, Peter Hugo

    2003-12-01

    The potassium channel from Streptomyces lividans (KcsA) is an integral membrane protein with sequence similarity to all known potassium channels, particularly in the selectivity filter region. A recently proposed model for ion channels containing either n or (n-1) single-file ions in their selectivity filters [P. H. Nelson, J. Chem. Phys. 177, 11396 (2002)] is applied to published KcsA channel K+ permeation data that exhibit a high-affinity process at low concentrations and a low-affinity process at high concentrations [M. LeMasurier et al., J. Gen. Physiol. 118, 303 (2001)]. The kinetic model is shown to provide a reasonable first-order explanation for both the high- and low-concentration permeation modes observed experimentally. The low-concentration mode ([K+]200 mM) has a 200-mV dissociation constant of 1100 mM and a conductance of 500 pS. Based on the permeation model, and x-ray analysis [J. H. Morais-Cabral et al., Nature (London) 414, 37 (2001)], it is suggested that the experimentally observed K+ permeation modes correspond to an n=3 mechanism at high concentrations and an n=2 mechanism at low concentrations. The ratio of the electrical dissociation distances for the high- and low-concentration modes is 3:2, also consistent with the proposed n=3 and n=2 modes. Model predictions for K+ channels that exhibit asymmetric current-voltage (I-V) curves are presented, and further validation of the kinetic model via molecular simulation and experiment is discussed. The qualitatively distinct I-V characteristics exhibited experimentally by Tl+, NH+4, and Rb+ ions at 100 mM concentration can also be explained using the model, but more extensive experimental tests are required for quantitative validation of the model predictions.

  8. Selective Permeation and Organic Extraction of Recombinant Green Fluorescent Protein (gfpuv from Escherichia coli

    Directory of Open Access Journals (Sweden)

    Ishii Marina

    2002-04-01

    Full Text Available Abstract Background Transformed cells of Escherichia coli DH5-α with pGFPuv, induced by IPTG (isopropyl-β-d-thiogalactopyranoside, express the green fluorescent protein (gfpuv during growth phases. E. coli subjected to the combination of selective permeation by freezing/thawing/sonication cycles followed by the three-phase partitioning extraction (TPP method were compared to the direct application of TPP to the same culture of E. coli on releasing gfpuv from the over-expressing cells. Material and Methods Cultures (37°C/100 rpm/ 24 h; μ = 0.99 h-1 - 1.10 h-1 of transformed (pGFP Escherichia coli DH5-α, expressing the green fluorescent protein (gfpuv, absorbance at 394 nm and emission at 509 nm were sonicated in successive intervals of sonication (25 vibrations/pulse to determine the maximum amount of gfpuv released from the cells. For selective permeation, the transformed previously frozen (-75°C cells were subjected to three freeze/thaw (-20°C/ 0.83°C/min cycles interlaid by sonication (3 pulses/ 6 seconds/ 25 vibrations. The intracellular permeate with gfpuv in extraction buffer (TE solution (25 mM Tris-HCl, pH 8.0, 1 mM β-mercaptoethanol β-ME, 0.1 mM PMSF was subjected to the three-phase partitioning (TPP method with t-butanol and 1.6 M ammonium sulfate. Sonication efficiency was verified on the application to the cells previously treated by the TPP method. The intra-cell releases were mixed and eluted through methyl HIC column with a buffer solution (10 mM Tris-HCl, 10 mM EDTA, pH 8.0. Results The sonication maximum released amount obtained from the cells was 327.67 μg gfpuv/mL (20.73 μg gfpuv/mg total proteins – BSA, after 9 min of treatment. Through the selective permeation by three repeated freezing/thawing/sonication cycles applied to the cells, a close content of 241.19 μg gfpuv/mL (29.74 μg gfpuv/mg BSA was obtained. The specific mass range of gfpuv released from the same cultures, by the three-phase partitioning (TPP

  9. Surface condition effects on tritium permeation through the first wall of a water-cooled ceramic breeder blanket

    Energy Technology Data Exchange (ETDEWEB)

    Zhou, H.-S. [Institute of Plasma Physics, Chinese Academy of Sciences, P.O. Box 1126, Hefei (China); Xu, Y.-P.; Liu, H.-D. [Science Island Branch of Graduate School, University of Science and Technology of China, P.O. Box 1126, Hefei (China); Liu, F.; Li, X.-C.; Zhao, M.-Z.; Qi, Q.; Ding, F. [Institute of Plasma Physics, Chinese Academy of Sciences, P.O. Box 1126, Hefei (China); Luo, G.-N., E-mail: gnluo@ipp.ac.cn [Institute of Plasma Physics, Chinese Academy of Sciences, P.O. Box 1126, Hefei (China); Science Island Branch of Graduate School, University of Science and Technology of China, P.O. Box 1126, Hefei (China); Hefei Center for Physical Science and Technology, P.O. Box 1126, Hefei (China); Hefei Science Center of Chinese Academy of Science, P.O. Box 1126, Hefei (China)

    2016-11-01

    Highlights: • We investigate surface effects on T transport through the first wall. • We solve transport equations with various surface conditions. • The RAFMs walls w/and w/o W exhibit different T permeation behavior. • Diffusion in W has been found to be the rate-limiting step. - Abstract: Plasma-driven permeation of tritium (T) through the first wall of a water-cooled ceramic breeder (WCCB) blanket may raise safety and other issues. In the present work, surface effects on T transport through the first wall of a WCCB blanket have been investigated by theoretical calculation. Two types of wall structures, i.e., reduced activation ferritic/martensitic steels (RAFMs) walls with and without tungsten (W) armor, have been analyzed. Surface recombination is assumed to be the boundary condition for both the plasma-facing side and the coolant side. It has been found that surface conditions at both sides can affect T permeation flux and inventory. For the first wall using W as armor material, T permeation is not sensitive to the plasma-facing surface conditions. Contamination of the surfaces will lead to higher T inventory inside the first wall.

  10. Permeation of sumatriptan succinate across human skin using multiple types of self-dissolving microneedle arrays fabricated from sodium hyaluronate.

    Science.gov (United States)

    Wu, Dan; Katsumi, Hidemasa; Quan, Ying-Shu; Kamiyama, Fumio; Kusamori, Kosuke; Sakane, Toshiyasu; Yamamoto, Akira

    2016-09-01

    Available formulations of sumatriptan succinate (SS) have low bioavailability or are associated with site reactions. We developed various types of self-dissolving microneedle arrays (MNs) fabricated from sodium hyaluronate as a new delivery system for SS and evaluated their skin permeation and irritation in terms of clinical application. In vitro permeation studies with human skin, physicochemical properties (needle length, thickness and density), and penetration enhancers (glycerin, sodium dodecyl sulfate and lauric acid diethanolamide) were investigated. SS-loaded high-density MNs of 800 µm in length were the optimal formulation and met clinical therapeutic requirements. Penetration enhancers did not significantly affect permeation of SS from MNs. Optical coherence tomography images demonstrated that SS-loaded high-density MNs (800 µm) uniformly created drug permeation pathways for the delivery of SS into the skin. SS-loaded high-density MNs induced moderate primary skin irritations in rats, but the skin recovered within 72 h of removal of the MNs. These findings suggest that high-density MNs of 800 µm in length are an effective and promising formulation for transdermal delivery of SS. To our knowledge, this is the first report of SS permeation across human skin using self-dissolving MNs.

  11. Low-Volatility Agent Permeation (LVAP) Verification and Validation Report

    Science.gov (United States)

    2015-05-01

    custody procedures were obtained from the ISO / IEC 17025 :2005 standard13 as well as the current version of Permeation and Analytical Solutions Branch...Testing and Calibration Laboratories; ISO / IEC 17025 :2005; International Organization for Standardization: Geneva, Switzerland, 2005. 14. Box, G.E...ECBC, as detailed in Section 6.4.4 The International Organization for Standardization ( ISO ) method, 5725-3 (1994), was used to calculate the standard

  12. Tritium permeation behavior through pyrolytic carbon in tritium production using high-temperature gas-cooled reactor for fusion reactors

    Directory of Open Access Journals (Sweden)

    H. Ushida

    2016-12-01

    Full Text Available Under tritium production method using a high-temperature gas-cooled reactor loaded Li compound, Li compound has to be coated by ceramic materials in order to suppress the spreading of tritium to the whole reactor. Pyrolytic carbon (PyC is a candidate of the coating material because of its high resistance for gas permeation. In this study, hydrogen permeation experiments using a PyC-coated isotropic graphite tube were conducted and hydrogen diffusivity, solubility and permeability were evaluated. Tritium permeation behavior through PyC-coated Li compound particles was simulated by using obtained data. Hydrogen permeation flux through PyC in a steady state is proportional to the hydrogen pressure and is larger than that through Al2O3 which is also candidate coating material. However, total tritium leak within the supposed reactor operation period through the PyC-coated Li compound particles is lower than that through the Al2O3-coated ones because the hydrogen absorption capacity in PyC is considerably larger than that in Al2O3.

  13. Tailored nanostructured platforms for boosting transcorneal permeation: Box-Behnken statistical optimization, comprehensive in vitro, ex vivo and in vivo characterization.

    Science.gov (United States)

    Elsayed, Ibrahim; Sayed, Sinar

    2017-01-01

    Ocular drug delivery systems suffer from rapid drainage, intractable corneal permeation and short dosing intervals. Transcorneal drug permeation could increase the drug availability and efficiency in the aqueous humor. The aim of this study was to develop and optimize nanostructured formulations to provide accurate doses, long contact time and enhanced drug permeation. Nanovesicles were designed based on Box-Behnken model and prepared using the thin film hydration technique. The formed nanodispersions were evaluated by measuring the particle size, polydispersity index, zeta potential, entrapment efficiency and gelation temperature. The obtained desirability values were utilized to develop an optimized nanostructured in situ gel and insert. The optimized formulations were imaged by transmission and scanning electron microscopes. In addition, rheological characters, in vitro drug diffusion, ex vivo and in vivo permeation and safety of the optimized formulation were investigated. The optimized insert formulation was found to have a relatively lower viscosity, higher diffusion, ex vivo and in vivo permeation, when compared to the optimized in situ gel. So, the lyophilized nanostructured insert could be considered as a promising carrier and transporter for drugs across the cornea with high biocompatibility and effectiveness.

  14. Tailored nanostructured platforms for boosting transcorneal permeation: Box–Behnken statistical optimization, comprehensive in vitro, ex vivo and in vivo characterization

    Science.gov (United States)

    Elsayed, Ibrahim; Sayed, Sinar

    2017-01-01

    Ocular drug delivery systems suffer from rapid drainage, intractable corneal permeation and short dosing intervals. Transcorneal drug permeation could increase the drug availability and efficiency in the aqueous humor. The aim of this study was to develop and optimize nanostructured formulations to provide accurate doses, long contact time and enhanced drug permeation. Nanovesicles were designed based on Box–Behnken model and prepared using the thin film hydration technique. The formed nanodispersions were evaluated by measuring the particle size, polydispersity index, zeta potential, entrapment efficiency and gelation temperature. The obtained desirability values were utilized to develop an optimized nanostructured in situ gel and insert. The optimized formulations were imaged by transmission and scanning electron microscopes. In addition, rheological characters, in vitro drug diffusion, ex vivo and in vivo permeation and safety of the optimized formulation were investigated. The optimized insert formulation was found to have a relatively lower viscosity, higher diffusion, ex vivo and in vivo permeation, when compared to the optimized in situ gel. So, the lyophilized nanostructured insert could be considered as a promising carrier and transporter for drugs across the cornea with high biocompatibility and effectiveness. PMID:29133980

  15. Corrosion and hydrogen permeation of A216 Grade WCA steel in hydrothermal magnesium-containing brines

    International Nuclear Information System (INIS)

    Haberman, J.H.; Frydrych, D.J.; Westerman, R.E.

    1988-03-01

    Corrosion rates determined at 1 month in 150/degree/C brine increased with magnesium concentration. The structure of the corrosion product, as determined by x-ray diffraction, depended upon the magnesium concentration. In brines with less than 10,000 ppM magnesium, the primary corrosion product had a spinel structure characteristic of magnetite or magnesioferrite. In brines containing magnesium concentrations greater than 20,000 ppM, the primary corrosion product had the amakinite structure characteristic of a complex iron-magnesium hydroxide. The high corrosion rates observed in brines containing high magnesium concentrations suggest that the corrosion products having the amakinite structure is less protective than corrosion products having the spinel structure. Corrosion rates in high-magnesium (inclusion) brine determined over a 6-month test duration were essentially constant. Hydrogen permeation rates observed in exposing mild steel to high-Mg/sup 2/plus// brine at 150/degree/C could be potentially damaging to a mild steel waste package container. The rate of hydrogen permeation was proportional to the brine flow rate in the autoclave. Thiourea additions to the brine increased the hydrogen permeation rate; sulfate and bromide ion additions did not. The maximum gaseous hydrogen pressure attainable is not known (based on 3Fe /plus/ 4H 2 O /plus/ Fe(sub 3)O /plus/ 4H 2 , would be /approximately/900 atmospheres), and the dependence of permeation rate on temperature is not known. 8 refs., 13 figs., 3 tabs

  16. Permeation of Telone EC through protective gloves.

    Science.gov (United States)

    Zainal, Hanaa; Que Hee, Shane S

    2005-09-30

    Telone is a potent fumigant that is based on the chlorinated unsaturated hydrocarbon, 1,3-dichloropropene (1,3-DCP). It is often applied without dilution and so poses severe inhalation and air pollution threats. Urinary metabolites of 1,3-DCP have been detected after Telone skin exposure, so that preventing dermal exposure is also important. The objective of the study was to assess if nitrile and multi-layer ("laminated") gloves provide adequate protection against Telone EC formulation. To accomplish this, disposable (Safeskin) and chemically resistant (Sol-Vex) nitrile and laminated (Barrier mark and Silver Shield) glove materials were challenged by Telone EC with hexane liquid collection in an ASTM-type I-PTC-600 permeation cell. Analyses of cis- and trans-1,3-DCP in the collection fluid at specified times were performed on a moderately polar capillary column by gas chromatography-electron capture detection. Telone EC caused microholes in both nitrile materials, though the chemically protective material was degraded slower than the disposable nitrile. The laminated gloves offered limited protection. Silver Shield protected best because 1.5-2.3 mg 1,3-DCP permeated by 8 h relative to 2.5-7.6 mg for Barrier, implying about 2.5 times more protection for 8 h. Even for Silver Shield, the extent of protection was inadequate as illustrated by a risk assessment of the skin exposure situation. The normalized breakthrough times for both types of laminated gloves varied between 27 and 60 min. It is recommended that Viton gloves still be worn for protection.

  17. A novel microdialysis-dissolution/permeation system for testing oral dosage forms

    DEFF Research Database (Denmark)

    Fong, Sophia Yui Kau; Poulsen, Jessie; Brandl, Martin

    2016-01-01

    A novel microdialysis-dissolution/permeation (M-D/P) system was developed for the biopharmaceutical assessment of oral drug formulations. This system consists of a side-by-side diffusion chamber, a microdialysis unit fixed within the dissolution chamber for continuous sampling, and a biomimetic P...

  18. Effect of helium irradiation on deuterium permeation behavior in tungsten

    Energy Technology Data Exchange (ETDEWEB)

    Uemura, Yuki; Sakurada, Shodai; Fujita, Hiroe; Azuma, Keisuke; Zhou, Quilai [Graduate School of Science & Technology, Shizuoka University, 836 Ohya, Suruga, Shizuoka, 422-8529 Japan (Japan); Hatano, Yuji [Hydrogen Isotope Research Center, University of Toyama, 3190 Gofuku, Toyama, 930-8555 Japan (Japan); Yoshida, Naoaki; Watanabe, Hideo [Institute for Applied Mechanics, Kyushu University, 6-1 Kasuga-koen, Kasuga, Fukuoka, 816-8580 Japan (Japan); Oyaizu, Makoto; Isobe, Kanetsugu [National Institutes for Quantum and Radiological Science and Technology, 2166 Obuchi, Rokkasho, Aomori, 039-3212 Japan (Japan); Shimada, Masashi [Idaho National Laboratory, 1955 N. Fremont Avenue, Idaho Falls, ID 83415 (United States); Buchenauer, Dean; Kolasinski, Robert [Sandia National Laboratories, Chemistry, Combustion and Materials Center, Livermore, CA 94550 (United States); Chikada, Takumi [Graduate School of Science & Technology, Shizuoka University, 836 Ohya, Suruga, Shizuoka, 422-8529 Japan (Japan); Oya, Yasuhisa, E-mail: oya.yasuhisa@shizuoka.ac.jp [Graduate School of Science & Technology, Shizuoka University, 836 Ohya, Suruga, Shizuoka, 422-8529 Japan (Japan)

    2017-07-15

    In this study, we measured deuterium (D) gas-driven permeation through tungsten (W) foils that had been pre-damaged by helium ions (He{sup +}). The goal of this work was to determine how ion-induced damage affects hydrogen isotope permeation. At 873 K, the D permeability for W irradiated by 3.0 keV He{sup +} was approximately one order of magnitude lower than that for un-damaged W. This difference diminished with increasing temperature. Even after heating to 1173 K, the permeability returned to less than half of the value measured for un-damaged W. We propose that this is due to nucleation of He bubbles near the surface which potentially serve as a barrier to diffusion deeper into the bulk. Exposure at higher temperatures shows that the D permeability and diffusion coefficients return to levels observed for undamaged material. It is possible that these effects are linked to annealing of defects introduced by ion damage, and whether the defects are stabilized by the presence of trapped He.

  19. Pervaporation membrane bioreactor with permeate fractional condensation and mechanical vapor compression for energy efficient ethanol production

    International Nuclear Information System (INIS)

    Fan, Senqing; Xiao, Zeyi; Li, Minghai; Li, Sizhong

    2016-01-01

    Graphical abstract: Pervaporation membrane bioreactor with permeate partial condensation and mechanical vapor compression is developed for an energy efficient ethanol production. - Highlights: • PVMBR-MVC for energy efficient ethanol production. • Process separation factor of 20–44 for ethanol achieved by fractional condensation. • Energy production of 20.25 MJ and hourly energy production of 56.25 kJ/h achieved. • Over 50% of energy saved in PVMBR-MVC compared with PVMBR-LTC. • Integrated heat pump with COP of 7–9 for the energy recovery of the permeate. - Abstract: Improved process separation factor and heat integration are two key issues to increase the energy efficiency of ethanol production in a pervaporation membrane bioreactor (PVMBR). A PVMBR with permeate fractional condensation and mechanical vapor compression was developed for energy efficient ethanol production. A condensation model based on the mass balance and thermodynamic equilibrium in the partial vacuum condenser was developed for predicting the purification performance of the permeate vapor. Three runs of ethanol fermentation-pervaporation experiment were carried out and ethanol concentration of higher than 50 wt% could be achieved in the final condensate, with the separation factor of the process for ethanol increased to 20. Ethanol production could be enhanced in the bioreactor and 17.1 MJ of the energy could be produced in per liter of fermentation broth, owing to 27.0 MJ/kg heating value of the recovered ethanol. Compared with the traditional pervaporation process with low temperature condensation for ethanol production, 50% of the energy would be saved in the process. The energy consumption would be further reduced, if the available energy of the permeate vapor was utilized by integrating the mechanical vapor compression heat pump.

  20. Permeation of gasoline, diesel, bioethanol (E85), and biodiesel (B20) fuels through six glove materials.

    Science.gov (United States)

    Chin, Jo-Yu; Batterman, Stuart A

    2010-07-01

    Biofuels and conventional fuels differ in terms of their evaporation rates, permeation rates, and exhaust emissions, which can alter exposures of workers, especially those in the fuel refining and distribution industries. This study investigated the permeation of biofuels (bioethanol 85%, biodiesel 20%) and conventional petroleum fuels (gasoline and diesel) through gloves used in occupational settings (neoprene, nitrile, and Viton) and laboratories (latex, nitrile, and vinyl), as well as a standard reference material (neoprene sheet). Permeation rates and breakthrough times were measured using the American Society for Testing and Materials F739-99 protocol, and fuel and permeant compositions were measured by gas chromatography/mass spectrometry. In addition, we estimated exposures for three occupational scenarios and recommend chemical protective clothing suitable for use with motor fuels. Permeation rates and breakthrough times depended on the fuel-glove combination. Gasoline had the highest permeation rate among the four fuels. Bioethanol (85%) had breakthrough times that were two to three times longer than gasoline through neoprene, nitrile Sol-Vex, and the standard reference materials. Breakthrough times for biodiesel (20%) were slightly shorter than for diesel for the latex, vinyl, nitrile examination, and the standard neoprene materials. The composition of permeants differed from neat fuels, e.g., permeants were significantly enriched in the lighter aromatics including benzene. Viton was the best choice among the tested materials for the four fuels tested. Among the scenarios, fuel truck drivers had the highest uptake via inhalation based on the personal measurements available in the literature, and gasoline station attendants had highest uptake via dermal exposure if gloves were not worn. Appropriate selection and use of gloves can protect workers from dermal exposures; however, current recommendations from the National Institute for Occupational Safety and

  1. Microstructural Study on Oxygen Permeated Arc Beads

    Directory of Open Access Journals (Sweden)

    Kuan-Heng Liu

    2015-01-01

    Full Text Available We simulated short circuit of loaded copper wire at ambient atmosphere and successfully identified various phases of the arc bead. A cuprous oxide flake was formed on the surface of the arc bead in the rapid solidification process, and there were two microstructural constituents, namely, Cu-κ eutectic structure and solutal dendrites. Due to the arc bead formed at atmosphere during the local equilibrium solidification process, the phase of arc bead has segregated to the cuprous oxide flake, Cu-κ eutectic, and Cu phase solutal dendrites, which are the fingerprints of the arc bead permeated by oxygen.

  2. APPLICATION OF A SURFACE-RENEWAL MODEL TO PERMEATE-FLUX DATA FOR CONSTANTPRESSURE CROSS-FLOW MICROFILTRATION WITH DEAN VORTICES

    Directory of Open Access Journals (Sweden)

    G. Idan

    2015-06-01

    Full Text Available AbstractThe introduction of flow instabilities into a microfiltration process can dramatically change several elements such as the surface-renewal rate, permeate flux, specific cake resistance, and cake buildup on the membrane in a positive way. A recently developed surface-renewal model for constant-pressure, cross-flow microfiltration (Hasan et al., 2013 is applied to the permeate-flux data reported by Mallubhotla and Belfort (1997, one set of which included flow instabilities (Dean vortices while the other set did not. The surface-renewal model has two forms - the complete model and an approximate model. For the complete model, the introduction of vortices leads to a 53% increase in the surface-renewal rate, which increases the limiting (i.e., steady-state permeate flux by 30%, decreases the specific cake resistance by 14.5% and decreases the limiting cake mass by 15.5% compared to operation without vortices. For the approximate model, a 50% increase in the value of surface renewal rate is shown due to vortices, which increases the limiting permeate flux by 30%, decreases the specific cake resistance by 10.5% and decreases the limiting cake mass by 13.7%. The cake-filtration version of the critical-flux model of microfiltration (Field et al., 1995 is also compared against the experimental permeate-flux data of Mallubhotla and Belfort (1997. Although this model can represent the data, the quality of its fit is inferior compared to that of the surface-renewal model.

  3. Thermal effect of periodical bakeout on tritium inventory in first wall and permeation to coolant in reactor life

    International Nuclear Information System (INIS)

    Nakahara, Katsuhiko

    1989-01-01

    In view of safety, it is very important to control the tritium inventory in first walls and permeation to the coolant. A time-dependent diffusion and temperature calculation code, TPERM, was developed. Using this code, a numerical study on the long term effects of the bakeout temperature on tritium inventory and tritium permeation to the coolant was made. In this study, an FER type first wall (stainless steel) was considered and a cyclic operation (one cycle includes a plasma burn phase and a bakeout phase) was assumed. The results are as follows: (i) There is almost no difference in the tritium inventory in the first wall between the operation with 150 0 C-bakeout and the continuous burning operation (without bakeout). In both cases there is not tritium permeation to the coolant at 5 years' integrated burn time. The 150 0 C-bakeout is effective to release tritium in the surface (to 0.1 mm depth) region on the plasma side, but it is not effective to decrease the tritium inventory over the reactor life. (ii) To decrease the tritium inventory, a bakeout at a temperature higher than 150 0 C is necessary. But a high temperature bakeout causes earlier tritium permeation to the coolant. (iii) From these results it is suggested that the decrease the tritium inventory over the reactor life by bakeout, some form of protection against tritium permeation or a decontamination device in the cooling (or bakeout) system becomes necessary. (orig.)

  4. Effects of hypothyroidism on vascular 125I-albumin permeation and blood flow in rats

    International Nuclear Information System (INIS)

    Tilton, R.G.; Pugliese, G.; Chang, K.; Speedy, A.; Province, M.A.; Kilo, C.; Williamson, J.R.

    1989-01-01

    Effects of hypothyroidism on vascular 125I-albumin permeation and on blood flow were assessed in multiple tissues of male Sprague-Dawley rats rendered hypothyroid by dietary supplementation with 0.5% (wt/wt) 2-thiouracil or by thyroidectomy. In both thiouracil-treated and thyroidectomized rats, body weights, kidney weight, arterial blood pressure, and pulse rate were decreased significantly v age-matched controls. After 10 to 12 weeks of thiouracil treatment, 125I-albumin permeation was increased significantly in the kidney, aorta, eye (anterior uvea, choroid, retina), skin, and new granulation tissue, remained unchanged in brain, sciatic nerve, and heart, and was decreased in forelimb skeletal muscle. A similar pattern was observed in thyroidectomized rats, except that increases in 125I-albumin permeation for all tissues were smaller than those observed in thiouracil-treated rats, and 125I-albumin permeation in retina did not differ from controls. In both thiouracil-treated and thyroidectomized rats, changes in blood flow (assessed with 15-microns, 85Sr-labeled microspheres) relative to the decrease in arterial blood pressure were indicative of a decrease in regional vascular resistance except in the choroid and in the kidney, in which vascular resistance was increased significantly. Glomerular filtration rate was decreased, but filtration fraction and urinary excretion of albumin remained unchanged by thiouracil treatment and thyroidectomy. These results indicate that vascular hemodynamics and endothelial cell barrier functional integrity are modulated in many different tissues by the thyroid. In view of the correspondence of hypothyroid- and diabetes-induced vascular permeability changes, these results raise the possibility that altered thyroid function in diabetes may play a role in the pathogenesis of diabetic vascular disease

  5. Effect of Bile Salt on Permeation Characteristics of the Oral Mucosal ...

    African Journals Online (AJOL)

    An attempt was made to study the effect of bile salt [sodium glycocholate (SG)] as a permeation enhancer on mucoadhesive buccal patches of diltiazem hydrochloride (anti-anginal drug) using various polymers like hydroxypropyl methyl cellulosee (HPMC), Eudragit RL100, ethyl cellulose alone and in combination with PVP.

  6. Prediction of Permeation Resistance of Protective Gloves, etc. from Solubility Parameters

    DEFF Research Database (Denmark)

    Henriksen, H. Risvig; Madsen, Jørgen Øgaard

    1997-01-01

    ). A starting point was the authoritative conclusion (Coletta et al., 1978), that permeation in protective clothing could not be predicted. As a spin off, the predictive concept indicated that new types of polymers sometimes should be incorporated to reach a reasonable (long) breakthrough time and (low...

  7. Dynamic dissolution-/permeation-testing of nano- and microparticle formulations of fenofibrate

    DEFF Research Database (Denmark)

    Sironi, Daniel; Rosenberg, Jörg; Bauer-Brandl, Annette

    2017-01-01

    -/ or nanoparticle-formulation was tested. Nondissolved nano-/microparticles served as a reservoir helping to maintain high levels of molecularly dissolved drug, which in turn caused high and constant permeation rates. The micelle-bound drug may also serve as a drug-reservoir, yet of subordinate importance as long...

  8. Determination of factor of hydrogen permeation reduction (PRF) for different protective coatings over vanadium

    International Nuclear Information System (INIS)

    Afanasyev, S.; Kulsartov, T.; Shestakov, V.; Chikhray, Y.; Smith, D.

    2002-01-01

    Selection of structural materials for liquid-metal system as well as for another system and constructions of nuclear energy plants must be carried out and based on specified demands depending on conditions of these materials functioning. Specific demand is its compatibility with liquid metals. Design of reactors with liquid-metal coolant (Li, PbLi 17 ) which reproduces tritium arise additional demand to structural materials. This demand is a creation of structural material or protective barrier with minimum acceptable value of tritium permeation through itself or with maximum permeation reduction factor (PRF). Vanadium and vanadium alloys are supposed to be use as a blanket structural material in such nuclear energy plants. Worked out at first stage of studies vanadium coatings should have stability of its characteristics at temperature 800 deg. C under influence of hydrogen. Given work shows the experimental results on testing of protective coatings over vanadium: glass-ceramic coating and CaO-base coating. PRF for every coating and its changes depending on thermo-capacity of vanadium sample with coating was determined by method of hydrogen permeation. The results of experiments would be used at the development of cooling loops of reactor core protection with liquid-metal coolant

  9. New experimental set-up and procedure for analyzing the dynamics of permeation of H2(g) across Pd-based metallic membranes

    International Nuclear Information System (INIS)

    Decaux, C.; Millet, P.; Decaux, C.

    2006-01-01

    Hydrogen permeation through metallic membranes is an industrial process used for purification purposes. Palladium-based alloys are generally used as permeation materials at operating temperatures above the critical value so that the metal-H system remains monophasic and that diffusion proceeds at sufficient rate. In state of the art systems, metallic membranes with typical thickness of a few tens of microns are used and rate limitation are generally attributed to atomic H transport by diffusion. Because of cost considerations, it is necessary to reduce the thickness of these membranes. In the micron thick range, surface contributions are expected to become rate determining, or at least to play an increasing role. Further, when the membrane is used on the exit side of a gas reformer to directly extract hydrogen, corrosion problems are expected to occur on the upstream side of the membrane. For all these reasons, there is a need to separately measure surface and bulk (diffusion) rate contributions to the overall permeation flux. A new experimental equipment has been specifically designed for this purpose. This setup can be operated in two different modes: for sorption or permeation experiments. In a typical sorption experiment, the metallic membrane is disposed in the reaction chamber and hydrogen is allowed to react from both side of the surface. This procedure allows the separate measurement of surface absorption and desorption resistances associated with the chemisorption step. In a typical permeation experiment, the membrane is mounted between two volume chambers, a pressurized hydrogen source reservoir and an empty sink reservoir. Initially, a difference of pressure is set between the two reservoirs. When the valve of the source tank is opened, H 2 (g) flows to the membrane and permeation proceeds until pressure reaches an equilibrium value. In both experimental configurations, gas pressure transients are synchronously sampled all along the experiment

  10. Corneal permeation properties of a charged lipid nanoparticle carrier containing dexamethasone

    Science.gov (United States)

    Ban, Junfeng; Zhang, Yan; Huang, Xin; Deng, Guanghan; Hou, Dongzhi; Chen, Yanzhong; Lu, Zhufen

    2017-01-01

    Drug delivery carriers can maintain effective therapeutic concentrations in the eye. To this end, we developed lipid nanoparticles (L/NPs) in which the surface was modified with positively charged chitosan, which engaged in hydrogen bonding with the phospholipid membrane. We evaluated in vitro corneal permeability and release characteristics, ocular irritation, and drug dynamics of modified and unmodified L/NPs in aqueous humor. The size of L/NPs was uniform and showed a narrow distribution. Corneal permeation was altered by the presence of chitosan and was dependent on particle size; the apparent permeability coefficient of dexamethasone increased by 2.7 and 1.8 times for chitosan-modified and unmodified L/NPs, respectively. In conclusion, a chitosan-modified system could be a promising method for increasing the ocular bioavailability of unmodified L/NPs by enhancing their retention time and permeation into the cornea. These findings provide a theoretical basis for the development of effective drug delivery systems in the treatment of ocular disease. PMID:28243093

  11. The permeation of tritium through 316L stainless steel with multiple coatings

    International Nuclear Information System (INIS)

    Yao Zhenyu; Hao Jiakun; Zhou Changshan; Shan Changqi; Yu Jinnan

    2000-01-01

    TiN + TiC + TiN and TiN + TiC + SiO 2 films were deposited on the surface of 316L austenitic stainless steel by means of physical vapor deposition (PVD). The thickness of the films was about 2-3 μm. The film is compact, oxidation-resistant, and has good adherence with the substrate below 500 deg. C. Tritium gas permeation of 316L with multiple films was examined, and it was found that the tritium permeability in 316L with a TiN + TiC + TiN film was 4-5 orders of magnitude lower, and in 316L with a TiN + TiC + SiO 2 film was 4-6 orders of magnitude lower than that in 316L with a Pd film at about 200-500 deg. C. At about 600 deg. C, the permeability of 316L with the multiple coating was 3-4 orders of magnitude lower than that in 316L with a Pd film. The result shows that the tritium permeation barrier is formed by multiple coating above 300 deg. C, and it is stable below 500 deg. C. However, the barrier is partly destroyed at about 600 deg. C because of oxidation; although this results in degradation of the barrier, it still plays a positive role. These films may be useful as coatings for the first wall, tritium blanket, and heat exchanger in fusion reactors for tritium permeation resistance

  12. Transcorneal permeation of diclofenac as a function of temperature from film formulation in presence of triethanolamine and benzalkonium chloride.

    Science.gov (United States)

    Mohapatra, Rajaram; Senapati, Sibananda; Sahoo, Chinmaya; Mallick, Subrata

    2014-11-01

    The objective of this report was to evaluate the transcorneal permeation of diclofenac potassium (DCP) as a function of temperature from hydroxypropyl methylcellulose (HPMC) matrix film containing triethanolamine (TEM) as plasticizer and benzalkonium chloride (BKC) as preservative. Activation energy (Ea), enthalpy (ΔH), entropy (ΔS) and free energy (ΔG) of permeation, diffusion and partition were evaluated to understand the underlying mechanism of permeation. Permeation improved with the presence of both the plasticizer and preservative compared to preservative alone. Further, increased amount of TEM in the film increased drug transport across the cornea. Decreased Ea value of the film supported the fact. Rise of temperature from 26 to 30, 34 and 40 °C increased permeation in all the films. Ocular residence of the film in vivo in the rabbit revealed that the film swelled by pronounced lachrymal fluid uptake and traces of hydrogel remained still at the end of 6 h of application. Absence of characteristic exothermic peak of the drug in the thermogram of film formulations indicated the molecular dispersion of drug in polymer matrix. Scanning electron microscopy indicated that the drug crystal size decreased with increasing concentration of TEM in presence of BKC due to effective wetting of drug particles by the polymer. Copyright © 2014 Elsevier B.V. All rights reserved.

  13. The coupling effect of gas-phase chemistry and surface reactions on oxygen permeation and fuel conversion in ITM reactors

    KAUST Repository

    Hong, Jongsup

    2015-08-01

    © 2015 Elsevier B.V. The effect of the coupling between heterogeneous catalytic reactions supported by an ion transport membrane (ITM) and gas-phase chemistry on fuel conversion and oxygen permeation in ITM reactors is examined. In ITM reactors, thermochemical reactions take place in the gas-phase and on the membrane surface, both of which interact with oxygen permeation. However, this coupling between gas-phase and surface chemistry has not been examined in detail. In this study, a parametric analysis using numerical simulations is conducted to investigate this coupling and its impact on fuel conversion and oxygen permeation rates. A thermochemical model that incorporates heterogeneous chemistry on the membrane surface and detailed chemical kinetics in the gas-phase is used. Results show that fuel conversion and oxygen permeation are strongly influenced by the simultaneous action of both chemistries. It is shown that the coupling somewhat suppresses the gas-phase kinetics and reduces fuel conversion, both attributed to extensive thermal energy transfer towards the membrane which conducts it to the air side and radiates to the reactor walls. The reaction pathway and products, in the form of syngas and C2 hydrocarbons, are also affected. In addition, the operating regimes of ITM reactors in which heterogeneous- or/and homogeneous-phase reactions predominantly contribute to fuel conversion and oxygen permeation are elucidated.

  14. Water diffusion in fluoropolymer-based fuel-cell electrolyte membranes investigated by radioactivated-tracer permeation technique

    International Nuclear Information System (INIS)

    Sawada, S.; Yamaki, T.; Asano, M.; Maekawa, Y.; Suzuki, A.; Terai, T.

    2011-01-01

    The self-diffusion coefficient of water, D, in proton exchange membranes (PEMs) based on crosslinkedpolytetrafluoroethylene (cPTFE) films was measured by a radioactivated-tracer permeation technique using tritium labeled water (HTO). The D value was found to increase with the water volume fraction of the PEM, φ, probably because the water-filled regions were more effectively interconnected with each other at high φ, allowing water permeation to be faster through a PEM. Interestingly, the grafted PEMs showed the lower D compared to that of Nafion in spite of their high φ. This would be caused by tortuous structures of transport pathways and a strong coulombic interaction between water and the negatively-charged sulfonate (SO 3 - ) groups. Heavyoxygen water (H 2 18 O) was also used in the similar permeation experiment to obtain the D. Since the HTO diffusion actually occurred not only by translational motion of water but also by intermolecular hydrogen-atom hopping, comparing the D of HTO with that of H 2 18 O was likely to give the information about the state of water in the PEMs. (orig.)

  15. Characterization and In Vitro Skin Permeation of Meloxicam-Loaded Liposomes versus Transfersomes

    Directory of Open Access Journals (Sweden)

    Sureewan Duangjit

    2011-01-01

    Full Text Available The goal of this study was to develop and evaluate the potential use of liposome and transfersome vesicles in the transdermal drug delivery of meloxicam (MX. MX-loaded vesicles were prepared and evaluated for particle size, zeta potential, entrapment efficiency (%EE, loading efficiency, stability, and in vitro skin permeation. The vesicles were spherical in structure, 90 to 140 nm in size, and negatively charged (−23 to −43 mV. The %EE of MX in the vesicles ranged from 40 to 70%. Transfersomes provided a significantly higher skin permeation of MX compared to liposomes. Fourier Transform Infrared Spectroscopy (FT-IR and Differential Scanning Calorimetry (DSC analysis indicated that the application of transfersomes significantly disrupted the stratum corneum lipid. Our research suggests that MX-loaded transfersomes can be potentially used as a transdermal drug delivery system.

  16. Chronological age affects the permeation of fentanyl through human skin in vitro

    DEFF Research Database (Denmark)

    Holmgaard, R; Benfeldt, E; Sorensen, J A

    2013-01-01

    AIM: To study the influence of chronological age on fentanyl permeation through human skin in vitro using static diffusion cells. Elderly individuals are known to be more sensitive to opioids and obtain higher plasma concentrations following dermal application of fentanyl compared to younger...... individuals. The influence of age - as an isolated pharmacokinetic term - on the absorption of fentanyl has not been previously studied. METHOD: Human skin from 30 female donors was mounted in static diffusion cells, and samples were collected during 48 h. Donors were divided into three age groups: ... and old age groups: 5,922 and 4,050 ng, respectively). Furthermore, the lag time and absorption rate were different between the three groups, with a significantly higher rate in the young participants versus the oldest participants. CONCLUSION: We demonstrate that fentanyl permeates the skin of young...

  17. Electro-Conductive Membranes for Permeation Enhancement and Fouling Mitigation: A Short Review

    Directory of Open Access Journals (Sweden)

    Patrizia Formoso

    2017-07-01

    Full Text Available The research on electro-conductive membranes has expanded in recent years. These membranes have strong prospective as key components in next generation water treatment plants because they are engineered in order to enhance their performance in terms of separation, flux, fouling potential, and permselectivity. The present review summarizes recent developments in the preparation of electro-conductive membranes and the mechanisms of their response to external electric voltages in order to obtain an improvement in permeation and mitigation in the fouling growth. In particular, this paper deals with the properties of electro-conductive polymers and the preparation of electro-conductive polymer membranes with a focus on responsive membranes based on polyaniline, polypyrrole and carbon nanotubes. Then, some examples of electro-conductive membranes for permeation enhancement and fouling mitigation by electrostatic repulsion, hydrogen peroxide generation and electrochemical oxidation will be presented.

  18. Electro-Conductive Membranes for Permeation Enhancement and Fouling Mitigation: A Short Review.

    Science.gov (United States)

    Formoso, Patrizia; Pantuso, Elvira; De Filpo, Giovanni; Nicoletta, Fiore Pasquale

    2017-07-28

    The research on electro-conductive membranes has expanded in recent years. These membranes have strong prospective as key components in next generation water treatment plants because they are engineered in order to enhance their performance in terms of separation, flux, fouling potential, and permselectivity. The present review summarizes recent developments in the preparation of electro-conductive membranes and the mechanisms of their response to external electric voltages in order to obtain an improvement in permeation and mitigation in the fouling growth. In particular, this paper deals with the properties of electro-conductive polymers and the preparation of electro-conductive polymer membranes with a focus on responsive membranes based on polyaniline, polypyrrole and carbon nanotubes. Then, some examples of electro-conductive membranes for permeation enhancement and fouling mitigation by electrostatic repulsion, hydrogen peroxide generation and electrochemical oxidation will be presented.

  19. Feasibility of permeation grouting for constructing subsurface barriers

    International Nuclear Information System (INIS)

    Dwyer, B.P.

    1994-03-01

    The technical feasibility of emplacing a barrier beneath a waste site using directionally drilled boreholes and permeation grouting was investigated. The benefits of this emplacement system are: (1) Directionally drilled boreholes provide access beneath a waste site without disturbing the waste; (2) interim containment of contaminants allows time for the development of remediation options; (3) in the interim, the volume of waste remains fixed; (4) barriers may enhance the effectiveness of in situ remediation actions; and (5) barrier systems may provide permanent waste containment

  20. Skin permeation and antioxidant efficacy of topically applied resveratrol.

    Science.gov (United States)

    Alonso, Cristina; Martí, M; Barba, C; Carrer, V; Rubio, L; Coderch, L

    2017-08-01

    The permeation of resveratrol was assessed by in vitro and in vivo experiments 24 h after topical administration. The in vitro profile of resveratrol was assessed by Raman spectroscopy. Human skin permeation was analysed in vivo by the tape stripping method with the progressive removal of the stratum corneum layers using adhesive tape strips. Moreover, the free radical scavenging activity of resveratrol after its topical application was determined using the DPPH assay. The Raman spectra indicated that the topically applied resveratrol penetrates deep into the skin. The results showed high amounts of resveratrol in the different stratum corneum layers close to the surface and a constant lower amount in the upper layers of the viable epidermis. The concentration of resveratrol present in the outermost stratum corneum layers was obtained by tape stripping after in vivo application. The results demonstrated that resveratrol mainly remained in the human stratum corneum layers. After topical application, resveratrol maintained its antiradical activity. The antioxidant efficacy of the compound was higher in the inner layers of the stratum corneum. As these results have demonstrated, topically applied resveratrol reinforces the antioxidant system of the stratum corneum and provides an efficient means of increasing the tissue levels of antioxidants in the human epidermis.

  1. Find_tfSBP: find thermodynamics-feasible and smallest balanced pathways with high yield from large-scale metabolic networks.

    Science.gov (United States)

    Xu, Zixiang; Sun, Jibin; Wu, Qiaqing; Zhu, Dunming

    2017-12-11

    Biologically meaningful metabolic pathways are important references in the design of industrial bacterium. At present, constraint-based method is the only way to model and simulate a genome-scale metabolic network under steady-state criteria. Due to the inadequate assumption of the relationship in gene-enzyme-reaction as one-to-one unique association, computational difficulty or ignoring the yield from substrate to product, previous pathway finding approaches can't be effectively applied to find out the high yield pathways that are mass balanced in stoichiometry. In addition, the shortest pathways may not be the pathways with high yield. At the same time, a pathway, which exists in stoichiometry, may not be feasible in thermodynamics. By using mixed integer programming strategy, we put forward an algorithm to identify all the smallest balanced pathways which convert the source compound to the target compound in large-scale metabolic networks. The resulting pathways by our method can finely satisfy the stoichiometric constraints and non-decomposability condition. Especially, the functions of high yield and thermodynamics feasibility have been considered in our approach. This tool is tailored to direct the metabolic engineering practice to enlarge the metabolic potentials of industrial strains by integrating the extensive metabolic network information built from systems biology dataset.

  2. Gas Sorption, Diffusion and Permeation in a Polymer of Intrinsic Microporosity (PIM-7)

    KAUST Repository

    Alaslai, Nasser Y.

    2013-05-08

    The entire world including Saudi Arabia is dependent on natural gas to provide new energy supplies for the future. Conventional ways for gas separation are expensive, and, hence, it is very important to reduce the cost and lower the energy consumption. Membrane technology is a relatively new separation process for natural gas purification with large growth potential, specifically for off-shore applications. The economics of any membrane separation process depend primarily on the intrinsic gas permeation properties of the membrane materials. All current commercial membranes for natural gas separation are made from polymers, which have several drawbacks, including low permeability, moderate selectivity, and poor stability in acid gas and hydrocarbon environments. The recent development of polymeric materials called “polymers of intrinsic microporosity” (PIMs) provide a new class of high-performance membrane materials that are anticipated to be used in natural gas separation processes including, but not limited to, acid gas removal and separation of hydrocarbons from methane. PIM-7 is an excellent example of a material from the PIMs series for gas separation. It was selected for this work since it has not been extensively tested for its gas permeation properties to date. Specifically, sorption and mixed-gas permeation data were not available for PIM-7 prior to this work. Sorption isotherms of N2, O2, CH4, CO2, C2H6, C3H8 and n-C4H10 were determined over a range of pressures at 35 oC for PIM-7 using a custom-designed dual-volume pressure decay system. Condensable hydrocarbon gases, such as C3H8 and n-C4H10, show significantly higher solubility than the other less condensable gas of the test series due to their high affinity to the polymer matrix. Dual-mode sorption model parameters were determined from the sorption isotherms. Henry’s law solubility, Langmuir capacity constant and the affinity constant increased with gas condensability. Permeability coefficients

  3. CO2 permeation properties of poly(ethylene oxide)-based segmented block copolymers

    NARCIS (Netherlands)

    Husken, D.; Visser, Tymen; Wessling, Matthias; Gaymans, R.J.

    2010-01-01

    This paper discusses the gas permeation properties of poly(ethylene oxide) (PEO)-based segmented block copolymers containing monodisperse amide segments. These monodisperse segments give rise to a well phase-separated morphology, comprising a continuous PEO phase with dispersed crystallised amide

  4. Development of a vacuum leak test method for large-scale superconducting magnet test facilities

    International Nuclear Information System (INIS)

    Kawano, Katsumi; Hamada, Kazuya; Okuno, Kiyoshi; Kato, Takashi

    2006-01-01

    Japan Atomic Energy Agency (JAEA) has developed leak detection technology for liquid helium temperature experiments in large-scale superconducting magnet test facilities. In JAEA, a cryosorption pump that uses an absorbent cooled by liquid nitrogen with a conventional helium leak detector, is used to detect helium gas that is leaking from pressurized welded joints of pipes and valves in a vacuum chamber. The cryosorption pump plays the role of decreasing aerial components, such as water, nitrogen and oxygen, to increase the sensitivity of helium leak detection. The established detection sensitivity for helium leak testing is 10 -10 to 10 -9 Pam 3 /s. A total of 850 welded and mechanical joints inside the cryogenic test facility for the ITER Central Solenoid Model Coil (CSMC) experiments have been tested. In the test facility, 73 units of glass fiber-reinforced plastic (GFRP) insulation break are used. The amount of helium permeation through the GFRP was recorded during helium leak testing. To distinguish helium leaks from insulation-break permeation, the helium permeation characteristic of the GFRP part was measured as a function of the time of helium charging. Helium permeation was absorbed at 6 h after helium charging, and the detected permeation is around 10 -7 Pam 3 /s. Using the helium leak test method developed, CSMC experiments have been successfully completed. (author)

  5. Concentration gradient driven molecular dynamics: a new method for simulations of membrane permeation and separation.

    Science.gov (United States)

    Ozcan, Aydin; Perego, Claudio; Salvalaglio, Matteo; Parrinello, Michele; Yazaydin, Ozgur

    2017-05-01

    In this study, we introduce a new non-equilibrium molecular dynamics simulation method to perform simulations of concentration driven membrane permeation processes. The methodology is based on the application of a non-conservative bias force controlling the concentration of species at the inlet and outlet of a membrane. We demonstrate our method for pure methane, ethane and ethylene permeation and for ethane/ethylene separation through a flexible ZIF-8 membrane. Results show that a stationary concentration gradient is maintained across the membrane, realistically simulating an out-of-equilibrium diffusive process, and the computed permeabilities and selectivity are in good agreement with experimental results.

  6. Permeation tubes. Their use in the preparation of controlled gas atmospheres

    International Nuclear Information System (INIS)

    Galianos, J. A.; Palomares, F.

    1981-01-01

    In this paper, the state of the art on the nature, construction and properties of the permeation tubes Is reviewed; after describing the main calibration procedures In use, examples of their application in the area of the atmospheric pollution are given, especially in the case of sulfur dioxides. (Author) 38 refs

  7. Enhancement of skin permeation of flurbiprofen via its transdermal patches using isopulegol decanoate (ISO-C10) as an absorption enhancer: pharmacokinetic and pharmacodynamic evaluation.

    Science.gov (United States)

    Chen, Yang; Quan, Peng; Liu, Xiaochang; Guo, Wenjia; Song, Wenting; Cun, Dongmei; Wang, Zhongyan; Fang, Liang

    2015-09-01

    The study aimed to prepare a transdermal patch for flurbiprofen using isopulegol decanoate (ISO-C10) as a permeation enhancer, and to evaluate the in-vitro and in-vivo percutaneous permeation of the drug, as well as the pharmacodynamic efficacy of the formulation. The permeation experiments were conducted on rabbit skin, and the pharmacokinetic profiles and synovial fluid drug concentration were measured after in-vivo transdermal administration. A deconvolution approach was employed to analyse the correlation between the in-vitro and in-vivo drug permeation. The anti-inflammatory and analgesic effects were, respectively, assessed using the adjuvant arthritis model and the acetic acid induced pain model. ISO-C10 could increase the in-vitro permeation of flurbiprofen from 46.22 ± 5.65 μg/cm(2) to 101.07 ± 10.85 μg/cm(2) . The in-vivo absorption of the drug was also improved by the enhancer, and a good linear correlation was observed between the in-vitro and in-vivo drug permeation. Meanwhile, the ISO-C10 contained patches increased the drug disposition in synovial fluid and enhanced the pharmacodynamic efficacy of the formulation. ISO-C10 would be a promising permeation enhancer for improving the in-vitro and in-vivo delivery of flurbiprofen from its transdermal patches. © 2015 Royal Pharmaceutical Society.

  8. What Is the Mechanism Behind Increased Permeation Rate of a Poorly Soluble Drug from Aqueous Dispersions of an Amorphous Solid Dispersion?

    DEFF Research Database (Denmark)

    Frank, K. J.; Westedt, U.; Rosenblatt, K. M.

    2014-01-01

    of amorphous microparticles present in aqueous dispersions induces lasting supersaturation maintaining enhanced permeation. The hypothesis is supported by a slower drug permeation when the microparticles were removed. (c) 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci...

  9. Gas-permeation properties of poly(ethylene oxide) poly(butylene terephthalate block copolymers

    NARCIS (Netherlands)

    Metz, S.J.; Mulder, M.H.V.; Wessling, Matthias

    2004-01-01

    This paper reports the gas-permeation properties of poly(ethylene oxide) (PEO) poly(butylene terephthalate) (PBT) segmented multiblock copolymers. These block copolymers allow a precise structural modification by the amount of PBT and the PEO segment length, enabling a systematic study of the

  10. Hydrogen permeation rate reduction by post-oxidation of aluminide coatings on DIN 1.4914 martensitic steel (MANET)

    International Nuclear Information System (INIS)

    Perujo, A.; Sample, T.

    1996-01-01

    In a previous work, it has been shown that lower aluminium content aluminide, having the same permeation rate reduction as the higher aluminium content, exhibited a lower hardness and greater ductility and therefore greater crack resistance than the higher aluminium content. In this work we combine this characteristic with a post-oxidation to obtain a further deuterium permeation reduction. The post-oxidation was performed in air at 1023 K for 15 h and at 1223 K for 10 h and 1 h. The maximum deuterium permeation rate reduction obtained is very moderate (maximum of a factor 500 for 1 h at 1223 K) as compared to that of the non-oxidised aluminide specimen (two orders of magnitude) and is constant in the temperature range studied (573-800 K). This method has the technological appeal of using air rather than the controlled environment used by other authors. (orig.)

  11. Hydrogen permeation through steel coated with erbium oxide by sol-gel method

    International Nuclear Information System (INIS)

    Yao Zhenyu; Suzuki, Akihiro; Levchuk, Denis; Chikada, Takumi; Tanaka, Teruya; Muroga, Takeo; Terai, Takayuki

    2009-01-01

    Er 2 O 3 coating is formed on austenitic stainless steel 316ss by sol-gel method. The results showed good crystallization of coating by baking in high purity flowing-argon at 973 K, and indicated that a little oxygen in baking atmosphere is necessary to crystallization of coating. The best baking temperature could be thought as 973 K, to get good crystallization of coating and avoid strong oxidation of steel substrate. The deuterium permeation test was performed for coated and bare 316ss, to evaluate the property of Er 2 O 3 sol-gel coating as a potential tritium permeation barrier. In this study, the deuterium permeability of coated 316ss is about 1-2 orders of magnitude lower than that of bare 316ss, and is about 2-3 orders of magnitude than the referred data of bare Eurofer97 and F82H martensitic steel.

  12. Dual-phase gas-permeation flow-injection thermometric analysis for the determination of carbon dioxide.

    Science.gov (United States)

    Liu, S J; Tubino, M

    1998-11-01

    A flow-injection configuration based on a dual-phase gas-permeation system from a liquid donor to a gas acceptor stream with a thermistor flow-through detector is proposed for the direct analysis of the gas in the acceptor. This system was applied for the determination of carbon dioxide (in the form of carbonate) using the following chemical reaction: CO(2)(g)+2NH(3)(g)+H(2)O(g)=(NH(4))(2)CO(3)(s), with a linear response from 1x10(-3) to 50x10(-3) mol l(-1) of CO(3)(2-). Carbon dioxide was produced in the liquid donor and permeated into the gaseous acceptor stream of air/water vapor. The detection limit is 1x10(-3) mol l(-1) of carbonate, and a sampling frequency of 60 h(-1) is achieved with a relative standard deviation of 4.1% for replicate injections. The dual-phase gas-permeation flow-injection manifold, along with the membrane and phase separations, as well as the chemical reaction, provides enhanced selectivity when compared with the system employing a liquid acceptor stream, as serious interferents in this system, for instance, acetate and formate, among others, do not interfere in the proposed system.

  13. Permeation measurement of gestodene for some biodegradable materials using Franz diffusion cells.

    Science.gov (United States)

    Liu, Danhua; Zhang, Chong; Zhang, Xiaowei; Zhen, Zhu; Wang, Ping; Li, Jianxin; Yi, Dongxu; Jin, Ying; Yang, Dan

    2015-09-01

    Biodegradable poly(d,l-lactide) (PDLLA), Poly(trimethylene carbonate) (PTMC), polycaprolactone (PCL), poly(caprolactone-co-d,l-lactide) (PCDLLA) and poly(trimethylene carbonate-co-caprolactone) (PTCL) are recently used for clinical drug delivery system such as subcutaneous contraceptive implant capsule due to their biodegradable properties that they could possess long-term stable performance in vivo without removal, however their permeation rate is unknown. In the work, biodegradable material membranes were prepared by solvent evaporation using chloroform, and commercial silicone rubber membrane served as a control. Gestodene was used as a model drug. Gestodene has high biologic progestational activity which allows for high contraceptive reliability at very low-dose levels. The permeation rate of gestodene for several biodegradable materials was evaluated. In vitro diffusion studies were done using Franz diffusion cells with a diffusion area of 1.33 cm(2). Phosphate buffer solution (PBS, pH 7.4), 10% methanol solution and distilled water were taken in donor and receiver chambers at temperature of 37 °C respectively. The in vitro experiments were conducted over a period of 24 h during which samples were collected at regular intervals. The withdrawn samples were appropriately diluted and measured on UV-vis spectrophotometer at 247 nm. Conclusion data from our study showed that permeation rate of PCDLLA with CL ratio more than 70% could be more excellent than commercial silicone rubber membrane. They may be suitable as a candidate carrier for gestodene subcutaneous contraceptive implants in contraceptive fields.

  14. Skin deposition and permeation of finasteride in vitro: effects of propylene glycol, ethanol and sodium lauryl sulfate.

    Science.gov (United States)

    Limpongsa, Ekapol; Jaipakdee, Napaphak; Pongjanyakul, Thaned

    2014-08-27

    Abstract The objective of this study was to investigate the effects of propylene glycol (PG), ethanol (EtOH) and sodium lauryl sulfate (SLS) on the in vitro deposition and permeation of finasteride (FNS). A side-by-side diffusion cell mounted with a pig ear skin and a saturated solution of FNS in PG (10, 20% v/v), EtOH (10, 20% v/v) or SLS (0.5, 1% w/v) vehicles were used. Incorporation of PG, EtOH or SLS caused a significant increase in FNS solubility both in the solution and on the skin with SLS > EtOH > PG. The results obtained from skin deposition studies showed that the FNS deposition rate and time increased in the same order as that of the solubility. The deposition kinetics of FNS solubilized in PG, EtOH and SLS vehicles followed either zero-order, square-root-of-time or pseudo-first-order kinetic models depending on the type and concentration of the enhancer. The permeation studies demonstrated that FNS permeation fluxes were enhanced only by EtOH vehicles. These results suggest that PG and SLS could be used as deposition enhancers, while EtOH could be the effective permeation enhancer of FNS. The obtained results can be used as the considerable insights for formulating the topical and transdermal products of FNS.

  15. Evaluation of the suitability of chromatographic systems to predict human skin permeation of neutral compounds.

    Science.gov (United States)

    Hidalgo-Rodríguez, Marta; Soriano-Meseguer, Sara; Fuguet, Elisabet; Ràfols, Clara; Rosés, Martí

    2013-12-18

    Several chromatographic systems (three systems of high-performance liquid chromatography and two micellar electrokinetic chromatography systems) besides the reference octanol-water partition system are evaluated by a systematic procedure previously proposed in order to know their ability to model human skin permeation. The precision achieved when skin-water permeability coefficients are correlated against chromatographic retention factors is predicted within the framework of the solvation parameter model. It consists in estimating the contribution of error due to the biological and chromatographic data, as well as the error coming from the dissimilarity between the human skin permeation and the chromatographic systems. Both predictions and experimental tests show that all correlations are greatly affected by the considerable uncertainty of the skin permeability data and the error associated to the dissimilarity between the systems. Correlations with much better predictive abilities are achieved when the volume of the solute is used as additional variable, which illustrates the main roles of both lipophilicity and size of the solute to penetrate through the skin. In this way, the considered systems are able to give precise estimations of human skin permeability coefficients. In particular, the HPLC systems with common C18 columns provide the best performances in emulating the permeation of neutral compounds from aqueous solution through the human skin. As a result, a methodology based on easy, fast, and economical HPLC measurements in a common C18 column has been developed. After a validation based on training and test sets, the method has been applied with good results to the estimation of skin permeation of several hormones and pesticides. Copyright © 2013 Elsevier B.V. All rights reserved.

  16. Effects of hypothyroidism on vascular /sup 125/I-albumin permeation and blood flow in rats

    Energy Technology Data Exchange (ETDEWEB)

    Tilton, R.G.; Pugliese, G.; Chang, K.; Speedy, A.; Province, M.A.; Kilo, C.; Williamson, J.R.

    1989-05-01

    Effects of hypothyroidism on vascular 125I-albumin permeation and on blood flow were assessed in multiple tissues of male Sprague-Dawley rats rendered hypothyroid by dietary supplementation with 0.5% (wt/wt) 2-thiouracil or by thyroidectomy. In both thiouracil-treated and thyroidectomized rats, body weights, kidney weight, arterial blood pressure, and pulse rate were decreased significantly v age-matched controls. After 10 to 12 weeks of thiouracil treatment, 125I-albumin permeation was increased significantly in the kidney, aorta, eye (anterior uvea, choroid, retina), skin, and new granulation tissue, remained unchanged in brain, sciatic nerve, and heart, and was decreased in forelimb skeletal muscle. A similar pattern was observed in thyroidectomized rats, except that increases in 125I-albumin permeation for all tissues were smaller than those observed in thiouracil-treated rats, and 125I-albumin permeation in retina did not differ from controls. In both thiouracil-treated and thyroidectomized rats, changes in blood flow (assessed with 15-microns, 85Sr-labeled microspheres) relative to the decrease in arterial blood pressure were indicative of a decrease in regional vascular resistance except in the choroid and in the kidney, in which vascular resistance was increased significantly. Glomerular filtration rate was decreased, but filtration fraction and urinary excretion of albumin remained unchanged by thiouracil treatment and thyroidectomy. These results indicate that vascular hemodynamics and endothelial cell barrier functional integrity are modulated in many different tissues by the thyroid. In view of the correspondence of hypothyroid- and diabetes-induced vascular permeability changes, these results raise the possibility that altered thyroid function in diabetes may play a role in the pathogenesis of diabetic vascular disease.

  17. Determination of trapping parameters and the chemical diffusion coefficient from hydrogen permeation experiments

    Czech Academy of Sciences Publication Activity Database

    Svoboda, Jiří; Mori, G.; Prethaler, A.; Fischer, F. D.

    2014-01-01

    Roč. 82, MAY (2014), s. 93-100 ISSN 0010-938X Institutional support: RVO:68081723 Keywords : Steel * Electrochemical calculation * Modeling studies * Hydrogen permeation * Kinetic parameters Subject RIV: BJ - Thermodynamics Impact factor: 4.422, year: 2014

  18. High-throughput hydrolysis of starch during permeation across {alpha}-amylase-immobilized porous hollow-fiber membranes

    Energy Technology Data Exchange (ETDEWEB)

    Miura, Suguru; Kubota, Noboru; Kawakita, Hidetaka; Saito, Kyoichi E-mail: marukyo@xtal.tf.chiba-u.ac.jp; Sugita, Kazuyuki; Watanabe, Kohei; Sugo, Takanobu

    2002-02-01

    Two kinds of supporting porous membranes, ethanolamine (EA) and phenol (Ph) fibers, for immobilization of {alpha}-amylase were prepared by radiation-induced graft polymerization of an epoxy-group-containing monomer, glycidyl methacrylate, onto a porous hollow-fiber membrane, and subsequent ring-opening with EA and Ph, respectively. An {alpha}-amylase solution was forced to permeate radially outward through the pores of the EA and Ph fibers. {alpha}-Amylase was captured at a density of 0.15 and 6.6 g/L of the membrane by the graft chain containing 2-hydroxyethylamino and phenyl groups, respectively. A permeation pressure of 0.10 MPa provided a space velocity of 780 and 1500 h{sup -1} for the {alpha}-amylase-immobilized EA and Ph fibers, respectively. Quantitative hydrolysis of starch during permeation of a 20 g/L starch solution in the buffer across the {alpha}-amylase-immobilized Ph fiber was attained up to a space velocity of about 2000 h{sup -1}; this was achieved because of negligible diffusional mass-transfer resistance of the starch to the {alpha}-amylase due to convective flow/ whereas an enzyme reaction-controlled system was observed for the {alpha}-amylase-immobilized EA fiber.

  19. Vapor permeation-stepwise injection simultaneous determination of methanol and ethanol in biodiesel with voltammetric detection.

    Science.gov (United States)

    Shishov, Andrey; Penkova, Anastasia; Zabrodin, Andrey; Nikolaev, Konstantin; Dmitrenko, Maria; Ermakov, Sergey; Bulatov, Andrey

    2016-02-01

    A novel vapor permeation-stepwise injection (VP-SWI) method for the determination of methanol and ethanol in biodiesel samples is discussed. In the current study, stepwise injection analysis was successfully combined with voltammetric detection and vapor permeation. This method is based on the separation of methanol and ethanol from a sample using a vapor permeation module (VPM) with a selective polymer membrane based on poly(phenylene isophtalamide) (PA) containing high amounts of a residual solvent. After the evaporation into the headspace of the VPM, methanol and ethanol were transported, by gas bubbling, through a PA membrane to a mixing chamber equipped with a voltammetric detector. Ethanol was selectively detected at +0.19 V, and both compounds were detected at +1.20 V. Current subtractions (using a correction factor) were used for the selective determination of methanol. A linear range between 0.05 and 0.5% (m/m) was established for each analyte. The limits of detection were estimated at 0.02% (m/m) for ethanol and methanol. The sample throughput was 5 samples h(-1). The method was successfully applied to the analysis of biodiesel samples. Copyright © 2015 Elsevier B.V. All rights reserved.

  20. High-throughput hydrolysis of starch during permeation across α-amylase-immobilized porous hollow-fiber membranes

    Science.gov (United States)

    Miura, Suguru; Kubota, Noboru; Kawakita, Hidetaka; Saito, Kyoichi; Sugita, Kazuyuki; Watanabe, Kohei; Sugo, Takanobu

    2002-02-01

    Two kinds of supporting porous membranes, ethanolamine (EA) and phenol (Ph) fibers, for immobilization of α-amylase were prepared by radiation-induced graft polymerization of an epoxy-group-containing monomer, glycidyl methacrylate, onto a porous hollow-fiber membrane, and subsequent ring-opening with EA and Ph, respectively. An α-amylase solution was forced to permeate radially outward through the pores of the EA and Ph fibers. α-Amylase was captured at a density of 0.15 and 6.6 g/L of the membrane by the graft chain containing 2-hydroxyethylamino and phenyl groups, respectively. A permeation pressure of 0.10 MPa provided a space velocity of 780 and 1500 h -1 for the α-amylase-immobilized EA and Ph fibers, respectively. Quantitative hydrolysis of starch during permeation of a 20 g/L starch solution in the buffer across the α-amylase-immobilized Ph fiber was attained up to a space velocity of about 2000 h -1; this was achieved because of negligible diffusional mass-transfer resistance of the starch to the α-amylase due to convective flow, whereas an enzyme reaction-controlled system was observed for the α-amylase-immobilized EA fiber.

  1. High-throughput hydrolysis of starch during permeation across α-amylase-immobilized porous hollow-fiber membranes

    International Nuclear Information System (INIS)

    Miura, Suguru; Kubota, Noboru; Kawakita, Hidetaka; Saito, Kyoichi; Sugita, Kazuyuki; Watanabe, Kohei; Sugo, Takanobu

    2002-01-01

    Two kinds of supporting porous membranes, ethanolamine (EA) and phenol (Ph) fibers, for immobilization of α-amylase were prepared by radiation-induced graft polymerization of an epoxy-group-containing monomer, glycidyl methacrylate, onto a porous hollow-fiber membrane, and subsequent ring-opening with EA and Ph, respectively. An α-amylase solution was forced to permeate radially outward through the pores of the EA and Ph fibers. α-Amylase was captured at a density of 0.15 and 6.6 g/L of the membrane by the graft chain containing 2-hydroxyethylamino and phenyl groups, respectively. A permeation pressure of 0.10 MPa provided a space velocity of 780 and 1500 h -1 for the α-amylase-immobilized EA and Ph fibers, respectively. Quantitative hydrolysis of starch during permeation of a 20 g/L starch solution in the buffer across the α-amylase-immobilized Ph fiber was attained up to a space velocity of about 2000 h -1 ; this was achieved because of negligible diffusional mass-transfer resistance of the starch to the α-amylase due to convective flow/ whereas an enzyme reaction-controlled system was observed for the α-amylase-immobilized EA fiber.

  2. The twisted ion-permeation pathway of a resting voltage-sensing domain.

    Science.gov (United States)

    Tombola, Francesco; Pathak, Medha M; Gorostiza, Pau; Isacoff, Ehud Y

    2007-02-01

    Proteins containing voltage-sensing domains (VSDs) translate changes in membrane potential into changes in ion permeability or enzymatic activity. In channels, voltage change triggers a switch in conformation of the VSD, which drives gating in a separate pore domain, or, in channels lacking a pore domain, directly gates an ion pathway within the VSD. Neither mechanism is well understood. In the Shaker potassium channel, mutation of the first arginine residue of the S4 helix to a smaller uncharged residue makes the VSD permeable to ions ('omega current') in the resting conformation ('S4 down'). Here we perform a structure-guided perturbation analysis of the omega conductance to map its VSD permeation pathway. We find that there are four omega pores per channel, which is consistent with one conduction path per VSD. Permeating ions from the extracellular medium enter the VSD at its peripheral junction with the pore domain, and then plunge into the core of the VSD in a curved conduction pathway. Our results provide a model of the resting conformation of the VSD.

  3. Preparation of Silica Nanoparticles Loaded with Nootropics and Their In Vivo Permeation through Blood-Brain Barrier

    Directory of Open Access Journals (Sweden)

    Josef Jampilek

    2015-01-01

    Full Text Available The blood-brain barrier prevents the passage of many drugs that target the central nervous system. This paper presents the preparation and characterization of silica-based nanocarriers loaded with piracetam, pentoxifylline, and pyridoxine (drugs from the class of nootropics, which are designed to enhance the permeation of the drugs from the circulatory system through the blood-brain barrier. Their permeation was compared with non-nanoparticle drug substances (bulk materials by means of an in vivo model of rat brain perfusion. The size and morphology of the nanoparticles were characterized by transmission electron microscopy. The content of the drug substances in silica-based nanocarriers was analysed by elemental analysis and UV spectrometry. Microscopic analysis of visualized silica nanocarriers in the perfused brain tissue was performed. The concentration of the drug substances in the tissue was determined by means of UHPLC-DAD/HRMS LTQ Orbitrap XL. It was found that the drug substances in silica-based nanocarriers permeated through the blood brain barrier to the brain tissue, whereas bulk materials were not detected in the brain.

  4. Preparation of silica nanoparticles loaded with nootropics and their in vivo permeation through blood-brain barrier.

    Science.gov (United States)

    Jampilek, Josef; Zaruba, Kamil; Oravec, Michal; Kunes, Martin; Babula, Petr; Ulbrich, Pavel; Brezaniova, Ingrid; Opatrilova, Radka; Triska, Jan; Suchy, Pavel

    2015-01-01

    The blood-brain barrier prevents the passage of many drugs that target the central nervous system. This paper presents the preparation and characterization of silica-based nanocarriers loaded with piracetam, pentoxifylline, and pyridoxine (drugs from the class of nootropics), which are designed to enhance the permeation of the drugs from the circulatory system through the blood-brain barrier. Their permeation was compared with non-nanoparticle drug substances (bulk materials) by means of an in vivo model of rat brain perfusion. The size and morphology of the nanoparticles were characterized by transmission electron microscopy. The content of the drug substances in silica-based nanocarriers was analysed by elemental analysis and UV spectrometry. Microscopic analysis of visualized silica nanocarriers in the perfused brain tissue was performed. The concentration of the drug substances in the tissue was determined by means of UHPLC-DAD/HRMS LTQ Orbitrap XL. It was found that the drug substances in silica-based nanocarriers permeated through the blood brain barrier to the brain tissue, whereas bulk materials were not detected in the brain.

  5. Evaluation of peracetic acid permeation during flash sterilization through pharmaceutical plastic polymers used in cytotoxic reconstitution units.

    Science.gov (United States)

    Havard, Laurent; Fellous-Jerome, Joelle; Bonan, Brigitte; Pradeau, Dominique; Prognon, Patrice

    2005-01-01

    Peracetic acid (PAA) permeation in flash sterilization was studied using three different plastic infusion bags made of polypropylene and polyethylene, filled with glucose 5% or NaCl 0.9%. The pH was measured and acetic acid (AA) and PAA concentrations were made by reverse phase high-performance liquid chromatography (RP-HPLC). PAA was derivatized by oxidation of methyl tolyl sulfide (MTS) into methyl tolyl sulfoxide (MTSO) detected by ultraviolet (UV) absorbance at 230 nm. The technique has a sensitivity of 0.3 microg x L(-1) and was highly specific. Results showed that pH measurements remain constant and demonstrated the absence of PAA permeation, which was confirmed by the absence of AA permeation regardless of the brand tested, with both unwrapped and overwrapped infusion bags, when flash sterilization is applied. These results allow flash sterilization to be performed with unwrapped infusion bags without any risk of drug degradation by PAA. This makes compounding safer and easier, which improves productivity.

  6. Calculation of tritium inventory and permeation in an INTOR like tokamak device and of its release after shut down

    International Nuclear Information System (INIS)

    Wienhold, P.; Waelbroeck, F.s; Winter, J.; Ali-Khan, I.

    1980-12-01

    The tritium inventory in the wall, its escape via permeation and its release in general are evaluated during the operation and later shut-down phases of a tokamak like INTOR by means of the PERI code. No real tritium problem arises after shut-down: simple outgasing techniques at wall temperatures Tsub(W) of approx. 500 0 C should decrease the tritium release rate down to a sufficient low level. Very serious problems are on the other hand expected to arise in the areas of inventory and permeation of tritium during the operation phase: one has the choice between e.g. operating at Tsub(W) = 350 0 C with a quasi-stationary tritium inventory of some 100 grams and a permeation flux phisub(p) approx. 1 gram/day or increasing Tsub(W) to 500 0 C, reducing the inventory down to some grams, but have then a leakage rate by permeation of 35 grams/day. For Tsub(W) = 100 0 C the tritium release into the torus will be intolerable. These figures are confirmed by preliminary measurements on Inconel 600. A suggestion is made as to how to alleviate the problem. (orig.) [de

  7. Evaluation of a Silicone Membrane as an Alternative to Human Skin for Determining Skin Permeation Parameters of Chemical Compounds.

    Science.gov (United States)

    Uchida, Takashi; Yakumaru, Masafumi; Nishioka, Keisuke; Higashi, Yoshihiro; Sano, Tomohiko; Todo, Hiroaki; Sugibayashi, Kenji

    2016-01-01

    We evaluated the effectiveness of a silicone membrane as an alternative to human skin using the skin permeation parameters of chemical compounds. An in vitro permeation study using 15 model compounds was conducted, and permeation parameters comprising permeability coefficient (P), diffusion parameter (DL(-2)), and partition parameter (KL) were calculated from each permeation profile. Significant correlations were obtained in log P, log DL(-2), and log KL values between the silicone membrane and human skin. DL(-2) values of model compounds, except flurbiprofen, in the silicone membrane were independent of the lipophilicity of the model compounds and were 100-fold higher than those in human skin. For antipyrine and caffeine, which are hydrophilic, KL values in the silicone membrane were 100-fold lower than those in human skin, and P values, calculated as the product of a DL(-2) and KL, were similar. For lipophilic compounds, such as n-butyl paraben and flurbiprofen, KL values for silicone were similar to or 10-fold higher than those in human skin, and P values for silicone were 100-fold higher than those in human skin. Furthermore, for amphiphilic compounds with log Ko/w values from 0.5 to 3.5, KL values in the silicone membrane were 10-fold lower than those in human skin, and P values for silicone were 10-fold higher than those in human skin. The silicone membrane was useful as a human skin alternative in an in vitro skin permeation study. However, depending on the lipophilicity of the model compounds, some parameters may be over- or underestimated.

  8. Tritium permeation model for plasma facing components

    Science.gov (United States)

    Longhurst, G. R.

    1992-12-01

    This report documents the development of a simplified one-dimensional tritium permeation and retention model. The model makes use of the same physical mechanisms as more sophisticated, time-transient codes such as implantation, recombination, diffusion, trapping and thermal gradient effects. It takes advantage of a number of simplifications and approximations to solve the steady-state problem and then provides interpolating functions to make estimates of intermediate states based on the steady-state solution. The model is developed for solution using commercial spread-sheet software such as Lotus 123. Comparison calculations are provided with the verified and validated TMAP4 transient code with good agreement. Results of calculations for the ITER CDA diverter are also included.

  9. Tritium permeation model for plasma facing components

    International Nuclear Information System (INIS)

    Longhurst, G.R.

    1992-12-01

    This report documents the development of a simplified one-dimensional tritium permeation and retention model. The model makes use of the same physical mechanisms as more sophisticated, time-transient codes such as implantation, recombination, diffusion, trapping and thermal gradient effects. It takes advantage of a number of simplifications and approximations to solve the steady-state problem and then provides interpolating functions to make estimates of intermediate states based on the steady-state solution. The model is developed for solution using commercial spread-sheet software such as Lotus 123. Comparison calculations are provided with the verified and validated TMAP4 transient code with good agreement. Results of calculations for the ITER CDA diverter are also included

  10. Study of radon 222 permeation through plastic membranes. Application to a measurement method of radon in water

    International Nuclear Information System (INIS)

    Labed, V.; Rannou, A.; Robe, M.C.

    1990-01-01

    Gaseous permeation is a complex phenomenon of gas transfer through some polymers. Original in respect of conventional studies where permeation occurs between two gaseous phases, the present study concerns radon 222 transfer between water and air through a membrane. Polypropylene membranes are tested with an experimental device following time evolution of the phenomenon by measurement of volume activity in water and in air. An application of this study to a method for determination of radon concentration in water by measurement of concentration in air is discussed [fr

  11. The Kinetics and the Permeation Properties of Piezo Channels.

    Science.gov (United States)

    Gnanasambandam, R; Gottlieb, P A; Sachs, F

    2017-01-01

    Piezo channels are eukaryotic, cation-selective mechanosensitive channels (MSCs), which show rapid activation and voltage-dependent inactivation. The kinetics of these channels are largely consistent across multiple cell types and different stimulation paradigms with some minor variability. No accessory subunits that associate with Piezo channels have been reported. They are homotrimers and each ∼300kD monomer has an N-terminal propeller blade-like mechanosensing module, which can confer mechanosensing capabilities on ASIC-1 (the trimeric non-MSC, acid-sensing ion channel-1) and a C-terminal pore module, which influences conductance, selectivity, and channel inactivation. Repeated stimulation can cause domain fracture and diffusion of these channels leading to synchronous loss of inactivation. The reconstituted channels spontaneously open only in asymmetric bilayers but lack inactivation. Mutations that cause hereditary xerocytosis alter PIEZO1 kinetics. The kinetics of the wild-type PIEZO1 and alterations thereof in mutants (M2225R, R2456K, and DhPIEZO1) are summarized in the form of a quantitative model and hosted online. The pore is permeable to alkali ions although Li + permeates poorly. Divalent cations, notably Ca 2+ , traverse the channel and inhibit the flux of monovalents. The large monovalent organic cations such as tetramethyl ammonium and tetraethyl ammonium can traverse the channel, but slowly, suggesting a pore diameter of ∼8Å, and the estimated in-plane area change upon opening is around 6-20nm 2 . Ruthenium red can enter the channel only from the extracellular side and seems to bind in a pocket close to residue 2496. Copyright © 2017 Elsevier Inc. All rights reserved.

  12. Efficient ethanol recovery from fermentation broths with integrated distillation-vapor permeation hybrid process

    Science.gov (United States)

    The energy demand of distillation-molecular sieve systems for ethanol recovery/dehydration can be significant, particularly for dilute solutions. An alternative hybrid process integrating vapor stripping (like a beer still) with vapor compression and a vapor permeation membrane s...

  13. Investigating the influence of diffusional coupling on mixture permeation across porous membranes

    NARCIS (Netherlands)

    Krishna, R.; van Baten, J.M.

    2013-01-01

    A careful analysis of published experimental data on permeation of a variety of binary mixtures reveals that there are fundamentally two types of diffusional coupling effects that need to be recognized. The first type of coupling occurs when the less-mobile species slows down its more mobile partner

  14. A 2D Finite Element Modelling of Tritium Permeation Through Cooling Plates for The HCLL DEMO Blanket Module

    International Nuclear Information System (INIS)

    Gabriel, F.; Escuriol, Y.; Dabbene, F.; Salavy, J.F.; Giancarli, L.; Gastaldi, O.

    2006-01-01

    As the Tritium self sufficiency is one of the major challenges for fusion reactor, breeding blankets represent one of the major technological breakthroughs required from passing from ITER to the next step reactor, usually called DEMO. One of the two blanket concepts developed in the EU is the Helium Cooled Lithium Lead (HCLL) blanket which uses the eutectic Pb-15.7Li metal liquid as both breeder and neutron multiplier. The structures, made of EUROFER, a low activation ferritic martensitic steel, are cooled by pressurized helium at 8 MPa and inlet/outlet temperature 300/500 o C. In this concept, the LiPb is fed from the top of the blanket and distributed in parallel vertical channels among pairs of cells (one cell for the radial movement towards the plasma, the other for the return). The liquid metal fills the in-box volume and is slowly re-circulated (few mm per seconds) to remove the produced tritium. In this paper, a local finite element modelling of the tritium permeation rate through the HCLL breeder unit cooling plates is presented. The tritium concentration in the helium circuit and remaining in the lithium lead circuit are evaluated by solving partial differential equations governing the tritium concentration balance, the thermal field and the lithium lead velocity field for a simplified 2D geometrical representation of the breeder unit. This allows estimating the sensitivity effect of coupling these different equations in order to deduce a relevant but simplified modelling for tritium permeation. This is to compare with tritium inventories studies, were the tritium permeation rate is estimated using simplified analytical modelling which generally leads to over estimate the tritium permeation rate to the coolant and so has strong influence on the coolant purification plant design. The finite element modelling performed shows that the Tritium permeation is considerable lower than the one obtained in previous estimations where nominal values of the governing

  15. Enhancement of Skin Permeation and Skin Immunization of Ovalbumin Antigen via Microneedles.

    Science.gov (United States)

    Pamornpathomkul, Boonnada; Rojanarata, Theerasak; Opanasopit, Praneet; Ngawhirunpat, Tanasait

    2017-10-01

    The purpose of this study was to evaluate the use of different types of microneedles and doses of ovalbumin antigen for in vitro skin permeation and in vivo immunization. In vitro skin permeation experiments and confocal laser scanning microscopy revealed that hollow microneedles had a superior enhancing effect on skin permeation compared with a solid microneedle patch and untreated skin by efficiently delivering ovalbumin-fluorescein conjugate into the deep skin layers. The flux and cumulative amount of ovalbumin-fluorescein conjugate at 8 h after administering with various conditions could be ranked as follows: hollow MN; high dose > medium dose > low dose > MN patch; high dose > medium dose > low dose > untreated skin; high dose > medium dose > low dose > without ovalbumin-fluorescein conjugate. As the dose of ovalbumin-fluorescein conjugate was increased to 500 μg, the antigen accumulated in the skin to a greater extent, as evidenced by the increasing green fluorescence intensity. When the hollow microneedle was used for the delivery of ovalbumin into the skin of mice, it was capable of inducing a stronger immunoglobulin G immune response than conventional subcutaneous injection at the same antigen dose. Immunoglobulin G levels in the hollow MN group were 5.7, 11.6, and 13.3 times higher than those of the subcutaneous injection group for low, medium, and high doses, respectively. Furthermore, the mice immunized using the hollow microneedle showed no signs of skin infection or pinpoint bleeding. The results suggest that the hollow MN is an efficient device for delivering the optimal dose of antigen via the skin for successful immunization.

  16. Permeation of platinum and rhodium nanoparticles through intact and damaged human skin

    International Nuclear Information System (INIS)

    Mauro, Marcella; Crosera, Matteo; Bianco, Carlotta; Adami, Gianpiero; Montini, Tiziano; Fornasiero, Paolo; Jaganjac, Morana; Bovenzi, Massimo; Filon, Francesca Larese

    2015-01-01

    The aim of the study was to evaluate percutaneous penetration of platinum and rhodium nanoparticles (PtNPs: 5.8 ± 0.9 nm, RhNPs: 5.3 ± 1.9 nm) through human skin. Salts compounds of these metals are sensitizers and some also carcinogenic agents. In vitro permeation experiments were performed using Franz diffusion cells with intact and damaged skin. PtNPs and RhNPs, stabilized with polyvinylpyrrolidone, were synthesized by reduction of Na 2 PtC l6 and RhCl 3 ·3H 2 O respectively. Suspensions with a concentration of 2.0 g/L of PtNPs and RhNPs were dispersed separately in synthetic sweat at pH 4.5 and applied as donor phases to the outer surface of the skin for 24 h. Measurements of the content of the metals in the receiving solution and in the skin were performed subsequently. Rhodium skin permeation was demonstrated through damaged skin, with a permeation flux of 0.04 ± 0.04 μg cm −2  h −1 and a lag time of 7.9 ± 1.1 h, while no traces of platinum were found in receiving solutions. Platinum and rhodium skin-analysis showed significantly higher concentrations of the metals in damaged skin. Rh and Pt applied as NPs can penetrate the skin barrier and Rh can be found in receiving solutions. These experiments pointed out the need for skin contamination prevention, since even a minor injury to the skin barrier can significantly increase penetration

  17. Permeation of platinum and rhodium nanoparticles through intact and damaged human skin

    Energy Technology Data Exchange (ETDEWEB)

    Mauro, Marcella [University of Trieste, Clinical Unit of Occupational Medicine, Department of Medical Sciences (Italy); Crosera, Matteo; Bianco, Carlotta; Adami, Gianpiero; Montini, Tiziano; Fornasiero, Paolo [University of Trieste, Department of Chemical and Pharmaceutical Sciences (Italy); Jaganjac, Morana [Rudjer Boskovic Institute, Laboratory for Oxidative Stress, Department of Molecular Medicine (Croatia); Bovenzi, Massimo; Filon, Francesca Larese, E-mail: larese@units.it [University of Trieste, Clinical Unit of Occupational Medicine, Department of Medical Sciences (Italy)

    2015-06-15

    The aim of the study was to evaluate percutaneous penetration of platinum and rhodium nanoparticles (PtNPs: 5.8 ± 0.9 nm, RhNPs: 5.3 ± 1.9 nm) through human skin. Salts compounds of these metals are sensitizers and some also carcinogenic agents. In vitro permeation experiments were performed using Franz diffusion cells with intact and damaged skin. PtNPs and RhNPs, stabilized with polyvinylpyrrolidone, were synthesized by reduction of Na{sub 2}PtC{sub l6} and RhCl{sub 3}·3H{sub 2}O respectively. Suspensions with a concentration of 2.0 g/L of PtNPs and RhNPs were dispersed separately in synthetic sweat at pH 4.5 and applied as donor phases to the outer surface of the skin for 24 h. Measurements of the content of the metals in the receiving solution and in the skin were performed subsequently. Rhodium skin permeation was demonstrated through damaged skin, with a permeation flux of 0.04 ± 0.04 μg cm{sup −2} h{sup −1} and a lag time of 7.9 ± 1.1 h, while no traces of platinum were found in receiving solutions. Platinum and rhodium skin-analysis showed significantly higher concentrations of the metals in damaged skin. Rh and Pt applied as NPs can penetrate the skin barrier and Rh can be found in receiving solutions. These experiments pointed out the need for skin contamination prevention, since even a minor injury to the skin barrier can significantly increase penetration.

  18. Gas separation process using membranes with permeate sweep to remove CO.sub.2 from gaseous fuel combustion exhaust

    Science.gov (United States)

    Wijmans, Johannes G [Menlo Park, CA; Merkel, Timothy C [Menlo Park, CA; Baker, Richard W [Palo Alto, CA

    2012-05-15

    A gas separation process for treating exhaust gases from the combustion of gaseous fuels, and gaseous fuel combustion processes including such gas separation. The invention involves routing a first portion of the exhaust stream to a carbon dioxide capture step, while simultaneously flowing a second portion of the exhaust gas stream across the feed side of a membrane, flowing a sweep gas stream, usually air, across the permeate side, then passing the permeate/sweep gas back to the combustor.

  19. Studies on the permeation of hydrogen through steam generator tubes at high temperatures using an electrochemical method

    International Nuclear Information System (INIS)

    Giraudeau, F.; Yang, L.; Steward, F.R.; DeBouvier, O.

    1998-01-01

    The permeation of hydrogen through steam generator tubes at high temperatures (∼ 300 degrees C) has been studied using an electrochemical technique. With this technique, hydrogen is generated on one side of the tube and monitored on the other side. The time for the hydrogen to reach the other side is used to determine the diffusion coefficient of hydrogen in the tube. Boundary conditions at the entry and exit sides have been investigated separately. Preliminary studies were performed on Stainless Steel 316 and Nickel Alloy 800 to better understand the influence of the solution chemistry on the electrochemical evolution of hydrogen. The surface phenomena effect and the trapping effect are discussed to account for differences observed in the permeation response. The hydrogen permeation through oxides at the exit side has been studied. Two nickel alloys (Alloy 800 and Alloy 600), materials widely used for steam generator tubes, have been investigated. The tubes were prefilmed using two different treatments. The oxides were formed in dry air at high temperatures (300 degrees C to 600 degrees C), or in humid gas at 300 degrees C. The diffusion coefficients at 300 degrees C in Stainless Steel 316 and Alloy 800 were determined to be of the order of 10 -6 - 10 -7 cm 2 /s for the bare metal. This is in agreement with results obtained by gas phase permeation techniques in the literature. (author)

  20. Physicochemical Characterization and Skin Permeation of Cationic Transfersomes Containing the Synthetic Peptide PnPP-19.

    Science.gov (United States)

    Almeida, Flavia De Marco; Silva, Carolina Nunes; de Araujo Lopes, Savia Caldeira; Santos, Daniel Moreira; Torres, Fernanda Silva; Cardoso, Felipe Lima; Martinelli, Patricia Massara; da Silva, Elizabeth Ribeiro; de Lima, Maria Elena; Miranda, Lucas Antonio Ferreira; Oliveira, Monica Cristina

    2018-01-08

    PnPP-19 is a 19-amino-acid synthetic peptide previously described as a novel drug for the treatment of erectile dysfunction. The aim of this work was to evaluate the physicochemical properties of cationic transfersomes containing PnPP-19 and the skin permeation of free PnPP-19 and PnPP-19-loaded transfersomes. Three different liposomal preparation methods were evaluated. Cationic transfersomes contained egg phosphatidyl choline: stearylamine (9:1 w/w) and Tween 20 (84.6:15.4 lipid:Tween, w/w). Lipid concentration varied from 20 to 40 mM. We evaluated the entrapment percentage, mean diameter, zeta potential and stability at 4 oC of the formulations. The skin permeation assays were performed with abdominal human skin using Franz diffusion cell with 3 cm2 diffusion area at 32 oC and a fluorescent derivative of the peptide, containing 5-TAMRA, bound to PnPP-19 C-terminal region, where an extra lysine was inserted. Our results showed variable entrapment efficiencies, from 6% to 30%, depending on the preparation method and the lipid concentration used. The reverse phase evaporation method using a total lipid concentration equal to 40 mM led to the best entrapment percentage (30.2 + 4.5%). Free PnPP-19 was able to permeate skin at a rate of 10.8 ng/cm2/h. However, PnPP-19 was specifically hydrolyzed by skin proteases, generating a fragment of 15 amino acid residues. Encapsulated PnPP-19 permeated the skin at a rate of 19.8 ng/cm2/h. The encapsulation of PnPP-19 in cationic transfersomes protected the peptide from degradation, favoring its topical administration. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  1. Vaginal drug delivery systems: A Review of Current Status | Dobaria ...

    African Journals Online (AJOL)

    Among the various routes of drug delivery, the vaginal route offers many advantages due to its large permeation area, rich vascularization, avoidance of first pass metabolism and relatively low enzymatic activity. Several studies have shown that the vaginal cavity is an effective route for drug administration intended mainly ...

  2. Use of alpha-tocopherol esters for topical vitamin E treatment: evaluation of their skin permeation and metabolism.

    Science.gov (United States)

    Ben-Shabat, Shimon; Kazdan, Yolia; Beit-Yannai, Elie; Sintov, Amnon C

    2013-05-01

    The aim of this work was to investigate new pro-vitamins based on α-tocopherol (α-Toc) and fatty acids, and to compare their properties with those of α-tocopherol acetate (α-TAc). Skin levels of α-Toc-fatty acid ester conjugates, total α-Toc and endogenous α-Toc were measured in skin samples taken from separate groups of treated and untreated rats. Multiple and extensive treatment with α-Toc oleate and α-TAc was also carried out to assess the skin accumulation and safety of these esters. The in-vivo studies revealed that α-Toc-fatty acid conjugates penetrated into the skin quantitatively while being comparable with the permeation of α-TAc. Differences were found between the levels of total α-Toc and endogenous α-Toc after application of α-TAc, α-Toc oleate, α-Toc linoleate, α-Toc-α linolenate and α-Toc palmitate, indicating that α-Toc conjugates of these fatty acids, but not α-Toc γ-linolenate or α-Toc stearate, were hydrolysed to free α-Toc. In long-term and extensive treatment, α-TAc was found to be lethal to rats treated with 1.15 mg/kg of this agent, which had been spread over 16 cm(2) of skin. Similar treatment with α-Toc oleate did not produce any side effects. This study suggests that α-Toc conjugates with unsaturated fatty acids may be a good alternative as stable vitamin E derivatives, rather than the α-TAc ester. © 2013 The Authors. JPP © 2013 Royal Pharmaceutical Society.

  3. Gender differences of brain glucose metabolic networks revealed by FDG-PET: evidence from a large cohort of 400 young adults.

    Science.gov (United States)

    Hu, Yuxiao; Xu, Qiang; Li, Kai; Zhu, Hong; Qi, Rongfeng; Zhang, Zhiqiang; Lu, Guangming

    2013-01-01

    Gender differences of the human brain are an important issue in neuroscience research. In recent years, an increasing amount of evidence has been gathered from noninvasive neuroimaging studies supporting a sexual dimorphism of the human brain. However, there is a lack of imaging studies on gender differences of brain metabolic networks based on a large population sample. FDG PET data of 400 right-handed, healthy subjects, including 200 females (age: 25:45 years, mean age ± SD: 40.9 ± 3.9 years) and 200 age-matched males were obtained and analyzed in the present study. We first investigated the regional differences of brain glucose metabolism between genders using a voxel-based two-sample t-test analysis. Subsequently, we investigated the gender differences of the metabolic networks. Sixteen metabolic covariance networks using seed-based correlation were analyzed. Seven regions showing significant regional metabolic differences between genders, and nine regions conventionally used in the resting-state network studies were selected as regions-of-interest. Permutation tests were used for comparing within- and between-network connectivity between genders. Compared with the males, females showed higher metabolism in the posterior part and lower metabolism in the anterior part of the brain. Moreover, there were widely distributed patterns of the metabolic networks in the human brain. In addition, significant gender differences within and between brain glucose metabolic networks were revealed in the present study. This study provides solid data that reveal gender differences in regional brain glucose metabolism and brain glucose metabolic networks. These observations might contribute to the better understanding of the gender differences in human brain functions, and suggest that gender should be included as a covariate when designing experiments and explaining results of brain glucose metabolic networks in the control and experimental individuals or patients.

  4. Gel in core carbosomes as novel ophthalmic vehicles with enhanced corneal permeation and residence.

    Science.gov (United States)

    Moustafa, Mona A; El-Refaie, Wessam M; Elnaggar, Yosra S R; Abdallah, Ossama Y

    2018-05-17

    Carbopol is a good bio-adhesive polymer that increases the residence time in the eye. However, the effect of blinking and lacrimation still reduce the amount of polymer and the incorporated drug available for bioadhesion. Gel-core liposomes are advanced systems offering benefits making it a good tool for improved ocular drug delivery and residence time. Incorporation of carbopol in gel-core liposomes and their potential in ocular delivery have not so far been investigated. Fluconazole (FLZ) was selected as a challenging important ocular antifungal suffering from poor corneal permeation and short residence time. In this study, gel-core carbosomes have been elaborated as novel carbopol-based ophthalmic vehicles to solve ocular delivery obstacles of FLZ and to sustain its effect. Full in vitro appraisal was performed considering gel-core structure, entrapment efficiency, particle size and stability of the vesicles as quality attributes. Structure elucidation of the nanocarrier was performed using optical, polarizing and transmission electron microscopy before and after Triton-X100 addition. Ex-vivo ocular permeation and in vivo performance were investigated on male albino rabbits. Optimized formulation (CBS5) showed gel-core structure, nanosize (339.00 ± 5.50 nm) and not defined before (62.00% ± 1.73) entrapment efficiency. Cumulative amount of CBS5 permeated ex-vivo after 6 h, was 2.43 and 3.43 folds higher than that of conventional liposomes and FLZ suspension, respectively. In-vivo corneal permeation of CBS5 showed significantly higher AUC0-24 h (487.12 ± 74.80) compared to that of FLZ suspension (204.34 ± 7.46) with longer residence time in the eye lasts for more than 18 h. In conclusion, novel gel-core carbosomes could successfully be used as a promising delivery system for chronic ocular diseases. Copyright © 2018 Elsevier B.V. All rights reserved.

  5. Fumarylacetoacetate hydrolase deficient pigs are a novel large animal model of metabolic liver disease

    Directory of Open Access Journals (Sweden)

    Raymond D. Hickey

    2014-07-01

    FAH-deficiency produced a lethal defect in utero that was corrected by administration of 2-(2-nitro-4-trifluoromethylbenzoyl-1,3 cyclohexanedione (NTBC throughout pregnancy. Animals on NTBC were phenotypically normal at birth; however, the animals were euthanized approximately four weeks after withdrawal of NTBC due to clinical decline and physical examination findings of severe liver injury and encephalopathy consistent with acute liver failure. Biochemical and histological analyses, characterized by diffuse and severe hepatocellular damage, confirmed the diagnosis of severe liver injury. FAH−/− pigs provide the first genetically engineered large animal model of a metabolic liver disorder. Future applications of FAH−/− pigs include discovery research as a large animal model of HT1 and spontaneous acute liver failure, and preclinical testing of the efficacy of liver cell therapies, including transplantation of hepatocytes, liver stem cells, and pluripotent stem cell-derived hepatocytes.

  6. Tritium management and anti-permeation strategies for three different breeding blanket options foreseen for the European Power Plant Physics and Technology Demonstration reactor study

    Energy Technology Data Exchange (ETDEWEB)

    Demange, D., E-mail: david.demange@kit.edu [Karlsruhe Institute of Technology, Institute for Technical Physics, Tritium Laboratory Karlsruhe, Herrmann-von-Helmholtz-Platz 1, D-76344 Eggenstein-Leopoldshafen (Germany); Boccaccini, L.V.; Franza, F. [Karlsruhe Institute of Technology, Institute for Neutron Physics and Reactor Technology, Herrmann-von-Helmholtz-Platz 1, D-76344 Eggenstein-Leopoldshafen (Germany); Santucci, A.; Tosti, S. [Associazione ENEA-Euratom sulla Fusione, C.R. ENEA Frascati, Via E. Fermi 45, 00044 Frascati (RM) (Italy); Wagner, R. [Karlsruhe Institute of Technology, Institute for Technical Physics, Tritium Laboratory Karlsruhe, Herrmann-von-Helmholtz-Platz 1, D-76344 Eggenstein-Leopoldshafen (Germany)

    2014-10-15

    In DT fusion reactors like DEMO, the commonly accepted tritium (T) losses through the steam generator (SG) shall not exceed about 2 mg/d that are more than 5 orders of magnitude lower than the T production rate of about 360 g/d in the breeding blanket (BB). A very effective mitigation strategy is required balancing the size and efficiency of the processes in the breeding and cooling loops, and the availability and efficiency of anti-permeation barriers. A numerical study is presented using the T permeation code FUS-TPC that computes all T flows and inventories considering the design and operation of the BB, the SG, and the T systems. Many scenarios are numerically analyzed for three breeding blankets concepts – helium cooled pebbles bed (HCPB), helium cooled lithium lead (HCLL), and water cooled lithium lead (WCLL) – varying the T processes throughput and efficiency, and the permeation regimes through the BB and SG to be either surface-limited or diffusion-limited with possible permeation reduction factor. For each BB concept, we discuss workable operation scenarios and suggest specific anti-permeation strategies.

  7. Preparation of Essential Oil-Based Microemulsions for Improving the Solubility, pH Stability, Photostability, and Skin Permeation of Quercetin.

    Science.gov (United States)

    Lv, Xia; Liu, Tiantian; Ma, Huipeng; Tian, Yan; Li, Lei; Li, Zhen; Gao, Meng; Zhang, Jianbin; Tang, Zeyao

    2017-11-01

    Quercetin can bring many benefits to skin based on its various bioactivities. However, the therapeutic effect of quercetin is limited due to the poor water solubility, pH instability, light instability, and skin permeation. The aim of the present work was applying essential oil-based microemulsions to improve the solubility, pH stability, photostability, and skin permeation of quercetin for topical application. Peppermint oil (PO-ME), clove oil (CO-ME), and rosemary oil (RMO-ME) were selected as model essential oils. Microemulsions composed of Cremophor EL/1,2-propanediol/essential oils (47:23:30, w/w) were selected as model formulations, based on the pseudo-ternary phase diagram and the characterizations. In the solubility study, the solubility of quercetin was improved dozens of times by microemulsions. Quercetin was found instable under alkaline condition, with 50% degraded in the solution of pH 13. However, PO-ME, CO-ME, and RMO-ME could protect quercetin from the hydroxide ions, with 47, 9, and 12% of quercetin degraded. In the photostability study, the essential oil-based microemulsions showed the capability of protecting quercetin from degradation under UV radiation. Where more than 67% of quercetin was degraded in aqueous solution, while less than 7% of quercetin degraded in microemulsions. At last, the in vitro skin permeation study showed that the essential oil-based microemulsions could enhance the permeation capacity of quercetin by 2.5-3 times compared to the aqueous solution. Hence, the prepared essential oil microemulsions could improve the solubility, pH stability, photostability, and skin permeation of quercetin, which will be beneficial for its topical application.

  8. Skin permeation enhancement effects of the gel and whole-leaf materials of Aloe vera, Aloe marlothii and Aloe ferox.

    Science.gov (United States)

    Fox, Lizelle T; Gerber, Minja; du Preez, Jan L; du Plessis, Jeanetta; Hamman, Josias H

    2015-01-01

    The aim of this study was to investigate the in-vitro permeation enhancement effects of the gel and whole-leaf materials of Aloe vera, Aloe marlothii and Aloe ferox using ketoprofen as a marker compound. The permeation studies were conducted across excised female abdominal skin in Franz diffusion cells, and the delivery of ketoprofen into the stratum corneum-epidermis and epidermis-dermis layers of the skin was investigated using a tape-stripping technique. A. vera gel showed the highest permeation-enhancing effect on ketoprofen (enhancement ratio or ER = 2.551) when compared with the control group, followed by A. marlothii gel (ER = 1.590) and A. ferox whole-leaf material (ER = 1.520). Non-linear curve fitting calculations indicated that the drug permeation-enhancing effect of A. vera gel can be attributed to an increased partitioning of the drug into the skin, while A. ferox whole leaf modified the diffusion characteristics of the skin for ketoprofen. The tape stripping results indicated that A. marlothii whole leaf delivered the highest concentration of the ketoprofen into the different skin layers. Of the selected aloe species investigated, A. vera gel material showed the highest potential as transdermal drug penetration enhancer across human skin. © 2014 Royal Pharmaceutical Society.

  9. Tritium permeation barrier based on self-healing composite materials

    International Nuclear Information System (INIS)

    Gao Jifeng; Zhang Dan; Suo Jinping

    2010-01-01

    Pores and cracks in ceramic coatings is one of the most important problems to be solved for the thermally sprayed tritium permeation barriers (TPBs) in fusion reactor. In this work, we developed a self-healing composite coating to address this problem. The coating composed of TiC + mixture(TiC/Al 2 O 3 ) + Al 2 O 3 was deposited on martensitic steels by means of atmospheric plasma spraying (APS). Before and after heat treatment, the morphology and phase of the coating were comparatively investigated by scanning electron microscopy (SEM) and X-ray diffraction (XRD). In the experiment, NiAl + Al 2 O 3 , mixture(TiC/Al 2 O 3 ) + Al 2 O 3 and NiAl + TiC + mixture(TiC/Al 2 O 3 ) + Al 2 O 3 films were also fabricated and studied, respectively. The results showed that the TiC + mixture(TiC/Al 2 O 3 ) + Al 2 O 3 coating exhibited the best self-healing ability and good thermal shock resistance among the four samples after heat treatment under normal atmosphere. The SEM images analyzed by Image Pro software indicated that the porosity of the TiC + mixture(TiC/Al 2 O 3 ) + Al 2 O 3 coating decreased more than 90% in comparison with the sample before heat treatment. This self-healing coating made by thermal spraying might be a good candidate for tritium permeation barrier in fusion reactors.

  10. Metabolic fingerprinting of fresh lymphoma samples used to discriminate between follicular and diffuse large B-cell lymphomas.

    Science.gov (United States)

    Barba, Ignasi; Sanz, Carolina; Barbera, Angels; Tapia, Gustavo; Mate, José-Luis; Garcia-Dorado, David; Ribera, Josep-Maria; Oriol, Albert

    2009-11-01

    To investigate if proton nuclear magnetic resonance ((1)H NMR) spectroscopy-based metabolic profiling was able to differentiate follicular lymphoma (FL) from diffuse large B-cell lymphoma (DLBCL) and to study which metabolites were responsible for the differences. High-resolution (1)H NMR spectra was obtained from fresh samples of lymph node biopsies obtained consecutively at one center (14 FL and 17 DLBCL). Spectra were processed using pattern-recognition methods. Discriminant models were able to differentiate between the two tumor types with a 86% sensitivity and a 76% specificity; the metabolites that most contributed to the discrimination were a relative increase of alanine in the case of DLBCL and a relative increase of taurine in FL. Metabolic models had a significant but weak correlation with Ki67 expression (r(2)=0.42; p=0.002) We have proved that it is possible to differentiate between FL and DLBCL based on their NMR metabolic profiles. This approach may potentially be applicable as a noninvasive tool for diagnostic and treatment follow-up in the clinical setting using conventional magnetic resonance systems.

  11. Comprehensive metabolic characterization of serum osteocalcin action in a large non-diabetic sample.

    Directory of Open Access Journals (Sweden)

    Lukas Entenmann

    Full Text Available Recent research suggested a metabolic implication of osteocalcin (OCN in e.g. insulin sensitivity or steroid production. We used an untargeted metabolomics approach by analyzing plasma and urine samples of 931 participants using mass spectrometry to reveal further metabolic actions of OCN. Several detected relations between OCN and metabolites were strongly linked to renal function, however, a number of associations remained significant after adjustment for renal function. Intermediates of proline catabolism were associated with OCN reflecting the implication in bone metabolism. The association to kynurenine points towards a pro-inflammatory state with increasing OCN. Inverse relations with intermediates of branch-chained amino acid metabolism suggest a link to energy metabolism. Finally, urinary surrogate markers of smoking highlight its adverse effect on OCN metabolism. In conclusion, the present study provides a read-out of metabolic actions of OCN. However, most of the associations were weak arguing for a limited role of OCN in whole-body metabolism.

  12. Comprehensive metabolic characterization of serum osteocalcin action in a large non-diabetic sample.

    Science.gov (United States)

    Entenmann, Lukas; Pietzner, Maik; Artati, Anna; Hannemann, Anke; Henning, Ann-Kristin; Kastenmüller, Gabi; Völzke, Henry; Nauck, Matthias; Adamski, Jerzy; Wallaschofski, Henri; Friedrich, Nele

    2017-01-01

    Recent research suggested a metabolic implication of osteocalcin (OCN) in e.g. insulin sensitivity or steroid production. We used an untargeted metabolomics approach by analyzing plasma and urine samples of 931 participants using mass spectrometry to reveal further metabolic actions of OCN. Several detected relations between OCN and metabolites were strongly linked to renal function, however, a number of associations remained significant after adjustment for renal function. Intermediates of proline catabolism were associated with OCN reflecting the implication in bone metabolism. The association to kynurenine points towards a pro-inflammatory state with increasing OCN. Inverse relations with intermediates of branch-chained amino acid metabolism suggest a link to energy metabolism. Finally, urinary surrogate markers of smoking highlight its adverse effect on OCN metabolism. In conclusion, the present study provides a read-out of metabolic actions of OCN. However, most of the associations were weak arguing for a limited role of OCN in whole-body metabolism.

  13. Electrical insulator assembly with oxygen permeation barrier

    Science.gov (United States)

    Van Der Beck, Roland R.; Bond, James A.

    1994-01-01

    A high-voltage electrical insulator (21) for electrically insulating a thermoelectric module (17) in a spacecraft from a niobium-1% zirconium alloy wall (11) of a heat exchanger (13) filled with liquid lithium (16) while providing good thermal conductivity between the heat exchanger and the thermoelectric module. The insulator (21) has a single crystal alumina layer (SxAl.sub.2 O.sub.3, sapphire) with a niobium foil layer (32) bonded thereto on the surface of the alumina crystal (26) facing the heat exchanger wall (11), and a molybdenum layer (31) bonded to the niobium layer (32) to act as an oxygen permeation barrier to preclude the oxygen depleting effects of the lithium from causing undesirable niobium-aluminum intermetallic layers near the alumina-niobium interface.

  14. Oxygen permeation through Nafion 117 membrane and its impact on efficiency of polymer membrane ethanol fuel cell

    Science.gov (United States)

    Jablonski, Andrzej; Kulesza, Pawel J.; Lewera, Adam

    2011-05-01

    We investigate oxygen permeation through Nafion 117 membrane in a direct ethanol fuel cell and elucidate how it affects the fuel cell efficiency. An obvious symptom of oxygen permeation is the presence of significant amounts of acetaldehyde and acetic acid in the mixture leaving anode when no current was drawn from the fuel cell (i.e. under the open circuit conditions). This parasitic process severely lowers efficiency of the fuel cell because ethanol is found to be directly oxidized on the surface of catalyst by oxygen coming through membrane from cathode in the absence of electric current flowing in the external circuit. Three commonly used carbon-supported anode catalysts are investigated, Pt, Pt/Ru and Pt/Sn. Products of ethanol oxidation are determined qualitatively and quantitatively at open circuit as a function of temperature and pressure, and we aim at determining whether the oxygen permeation or the catalyst's activity limits the parasitic ethanol oxidation. Our results strongly imply the need to develop more selective membranes that would be less oxygen permeable.

  15. Effects of rosiglitazone on metabolic parameters and large artery sclerosis in nondiabetic patients with metabolic sydrome

    International Nuclear Information System (INIS)

    Shen Zhenhai; Lu Yun; Feng Yinbo; Jin Xian

    2010-01-01

    Objective: To observe the effects of rosiglitazone on metabolic parameters, carotid intimamedia thickness (IMT), brachial-ankle pulse wave velocity (baPWV) and ankle-brachial index (ABI) in nondiabetic patients with metabolic syndrome. Methods: Seventy-nine nondiabetic patients with metabolic syndrome were randomly divided into treatment group (n = 41) and control group (n = 38). The patients in treatment group were treated with rosiglitazone on the basis of life-style intervention, those in control group were treated with life-style intervention for 9 months. All patients were followed up every 3 months. Body mass index (BMI) ,waist circumference(WC), systolic blood pressure (SBP), diastolic blood pressure(DBP), fasting blood glucose (FBG), triglyceride (TG), total cholesterol (TC), highdendity lipoprotein (HDL-C), Low-density lipoprotein (LDL), high sensitivity C-reactive protein (hsCRP), HbA 1 C, fasting insulin (FIns), HOMA-IR, IMT, baPWV and ABI were measured in both groups before treatment and at the 6th, 9th month after treatment. Results: (1) After treatment with rosiglitazone for 6 months, FPG, TG, HDL-C, hsC RP, HbA 1 C, Fins and HOMA-IR in treatment group were improved (P 1 C, Fins and HOMA-IR got further improvement at the 9th month after treatment (P 1 C, Fins and HOMA-IR were changed significantly in treatment group compared to those in control group (P 1 C and HOMA-IR was independently related to the improvement of ABI by multivarient analysis. Conclusion: In nondiabetic patients with metabolic syndrome, rosiglitazone can significantly improve insulin resistance, correct metabolic disorders, has anti-inflammatory effect and retard atherosclerosis at some extent. (authors)

  16. Water vapor permeation and dehumidification performance of poly(vinyl alcohol)/lithium chloride composite membranes

    KAUST Repository

    Bui, Duc Thuan; Nida, Aqdas; Ng, Kim  Choon; Chua, Kian  Jon

    2015-01-01

    were observed for membranes with increased lithium chloride content up to 50%. The permeation and sorption properties of the membranes were investigated under different temperatures. The results provided a deeper insight into the membrane water vapor

  17. Novel diffusion cell for in vitro transdermal permeation, compatible with automated dynamic sampling

    NARCIS (Netherlands)

    Bosman, I.J; Lawant, A.L; Avegaart, S.R.; Ensing, K; de Zeeuw, R.A

    The development of a new diffusion cell for in vitro transdermal permeation is described. The so-called Kelder cells were used in combination with the ASPEC system (Automatic Sample Preparation with Extraction Columns), which is designed for the automation of solid-phase extractions (SPE). Instead

  18. Gas Permeation Related to the Moisture Sorption in Films of Glassy Hydrophilic Polymers

    NARCIS (Netherlands)

    Laksmana, F. L.; Kok, P. J. A. Hartman; Frijlink, H. W.; Vromans, H.; Maarschalk, K. Van Der Voort

    2010-01-01

    The purpose of this article is to elucidate the effect of integral sorption of moisture on gas permeation in glassy hydrophilic polymers. The oxygen and the simultaneous moisture sorption into various hydroxypropyl methylcellulose (HPMC) films were measured under a wide range of relative humidities

  19. Research on Crack-Filling Heat Treatment and Hydrogen Permeation Test of Self-healing Tritium Permeation Barriers

    Science.gov (United States)

    Liu, Dawei; Wang, Yan; Zhang, Ying; Ouyang, Taoyuan; Zhou, Tong; Fang, Xuanwei; Suo, Jinping

    2018-03-01

    A TiC + mixture (TiC/Al2O3 (1:1 wt.%)) +Al2O3 self-healing triple layer coating (TLC) was designed and manufactured by our group, and the crack-filling heat treatment process had been roughly explored in the past. In this work, the accelerating test with a thick TiC + mixture (TiC/Al2O3 (1:1 wt.%)) double-layer coating (DLC) was carried out. The DLC coating warped when the heat treatment temperature was lower than 550 °C, which was rare in similar researches, and it crushed into fan-shaped pieces when the treatment temperature was higher than 650 °C. The two different spalling failures were explained by weight gain, porosity and stress analyses. The heating rate had a significant effect. The bonding strength and hydrogen permeation of the TLC samples were also tested. Remaining at 650 °C for 40 h was proved to be an optimal crack-filling heat treatment process, considering the hydrogen resistance.

  20. Extraction of tritium from liquid lithium by permeation

    International Nuclear Information System (INIS)

    Alire, R.M.

    1978-01-01

    This paper assesses a method for extracting tritium from liquid lithium for specific application to the conceptual laser fusion reactor that uses a continuous lithium ''waterfall.'' The tritium diffuses through a refractory metal that contains a getter and is then stored in a hydride-forming alloy. There are various uncertainties with this method including helium-4 extraction, unknown impurities that may accumulate in liquid lithium, the effects of these impurities on tritium separation, and the maintenance of tritium-contaminated equipment. Our study indicates that major tritium losses will occur during equipment maintenance rather than as a result of permeation losses through the primary vessel

  1. Effects of water-channel attractions on single-file water permeation through nanochannels

    International Nuclear Information System (INIS)

    Xu, Yousheng; Zheng, Youqu; Tian, Xingling; Lv, Mei; He, Bing; Deng, Maolin; Xiu, Peng; Tu, Yusong

    2016-01-01

    Single-file transportation of water across narrow nanochannels such as carbon nanotubes has attracted much attention in recent years. Such permeation can be greatly affected by the water-channel interactions; despite some progress, this issue has not been fully explored. Herein we use molecular dynamics simulations to investigate the effects of water-channel attractions on occupancy, translational (transportation) and orientational dynamics of water inside narrow single-walled carbon nanotubes (SWNTs). We use SWNTs as the model nanochannels and change the strength of water-nanotube attractions to mimic the changes in the hydrophobicity/polarity of the nanochannel. We investigate the dependence of water occupancy inside SWNTs on the water-channel attraction and identify the corresponding threshold values for drying states, wetting-drying transition states, and stably wetting states. As the strength of water-channel attractions increases, water flow increases rapidly first, and then decreases gradually; the maximal flow occurs in the case where the nanochannel is predominately filled with the 1D water wire but with a small fraction of ‘empty states’, indicating that appropriate empty-filling (drying-wetting) switching can promote water permeation. This maximal flow is unexpected, since in traditional view, the stable and tight hydrogen-bonding network of the water wire is the prerequisite for high permeability of water. The underlying mechanism is discussed from an energetic perspective. In addition, the effect of water-channel attractions on reorientational dynamics of the water wire is studied, and a negative correlation between the flipping frequency of water wire and the water-channel attraction is observed. The underlying mechanism is interpreted in term of the axial total dipole moment of inner water molecules. This work would help to better understand the effects of water-channel attractions on wetting properties of narrow nanochannels, and on single

  2. Polycyclic aromatic hydrocarbons (PAHs) skin permeation rates change with simultaneous exposures to solar ultraviolet radiation (UV-S).

    Science.gov (United States)

    Hopf, Nancy B; Spring, Philipp; Hirt-Burri, Nathalie; Jimenez, Silvia; Sutter, Benjamin; Vernez, David; Berthet, Aurelie

    2018-05-01

    Road construction workers are simultaneously exposed to two carcinogens; solar ultraviolet (UV-S) radiation and polycyclic aromatic hydrocarbons (PAHs) in bitumen emissions. The combined exposure may lead to photogenotoxicity and enhanced PAH skin permeation rates. Skin permeation rates (J) for selected PAHs in a mixture (PAH-mix) or in bitumen fume condensate (BFC) with and without UV-S co-exposures were measured with in vitro flow-through diffusion cells mounted with human viable skin and results compared. Possible biomarkers were explored. Js were greater with UV-S for naphthalene, anthracene, and pyrene in BFC (0.08-0.1 ng/cm 2 /h) compared to without (0.02-0.26 ng/cm 2 /h). This was true for anthracene, pyrene, and chrysene in the PAH-mix. Naphthalene and benzo(a)pyrene (BaP) in the PAH-mix had greater Js without (0.97-13.01 ng/cm 2 /h) compared to with UV-S (0.40-6.35 ng/cm 2 /h). Time until permeation (T lags ) in the PAH-mix were generally shorter compared to the BFC, and they ranged from 1 to 13 h. The vehicle matrix could potentially be the reason for this discrepancy as BFC contains additional not identified substances. Qualitative interpretation of p53 suggested a dose-response with UV-S, and somewhat with the co-exposures. MMP1, p65 and cKIT were not exploitable. Although not statistically different, PAHs permeate human viable skin faster with simultaneous exposures to UV. Copyright © 2018 Elsevier B.V. All rights reserved.

  3. Metabolomic Analyses of Leishmania Reveal Multiple Species Differences and Large Differences in Amino Acid Metabolism.

    Directory of Open Access Journals (Sweden)

    Gareth D Westrop

    Full Text Available Comparative genomic analyses of Leishmania species have revealed relatively minor heterogeneity amongst recognised housekeeping genes and yet the species cause distinct infections and pathogenesis in their mammalian hosts. To gain greater information on the biochemical variation between species, and insights into possible metabolic mechanisms underpinning visceral and cutaneous leishmaniasis, we have undertaken in this study a comparative analysis of the metabolomes of promastigotes of L. donovani, L. major and L. mexicana. The analysis revealed 64 metabolites with confirmed identity differing 3-fold or more between the cell extracts of species, with 161 putatively identified metabolites differing similarly. Analysis of the media from cultures revealed an at least 3-fold difference in use or excretion of 43 metabolites of confirmed identity and 87 putatively identified metabolites that differed to a similar extent. Strikingly large differences were detected in their extent of amino acid use and metabolism, especially for tryptophan, aspartate, arginine and proline. Major pathways of tryptophan and arginine catabolism were shown to be to indole-3-lactate and arginic acid, respectively, which were excreted. The data presented provide clear evidence on the value of global metabolomic analyses in detecting species-specific metabolic features, thus application of this technology should be a major contributor to gaining greater understanding of how pathogens are adapted to infecting their hosts.

  4. Development of Fe-AI CVD coatings as tritium permeation barrier

    International Nuclear Information System (INIS)

    Chabrol, C.; Schuster, F.; Le Marois, G.; Serra, E.

    1998-01-01

    A specific method of pack-cementation has been developed in order to perform a CVD deposition of Fe-Al alloys on a martensitic steel at a temperature which respects its mechanical properties ( 2 Al 5 intermetallic phases thanks to a low pressure deposition and using a special cement containing Fe and Al. These coatings coated with an Al 2 O 3 top layer drastically reduce the permeation rate of deuterium with regards to the uncoated substrate. (authors)

  5. Studies of the permeation and diffusion of tritium and hydrogen in TFTR

    International Nuclear Information System (INIS)

    Garber, H.J.

    1975-10-01

    This report documents the main features of studies conducted on the permeation and diffusion of tritium and hydrogen through the stainless steel sections comprising the vacuum vessel of TFTR. The overall conclusion of these studies is that tritium releases to the environment resulting from TFTR operations under normal conditions will be very small, less than one curie per year. A basis is described for predicting the magnitudes of the applicable transport properties for tritium-austenitic stainless steel systems as derived from a survey of the technical literature on tritium transport. The key characteristics of the TFTR vacuum vessel that are involved in the permeation and diffusion calculations are given. Information is given regarding the contemplated plasma scenarios and associated required gas injection quantities. Various issues involved in the bakeout of the vacuum vessel are discussed; focussing principally on the problems associated with in-situ bakeout and related means to reduce outgassing from the TFTR vessel and vacuum pumping system hardware. The anticipated tritium releases are studied considering the diffusion transients

  6. Ionic conductivity of perovskite LaCoO3 measured by oxygen permeation technique

    NARCIS (Netherlands)

    Chen, C.H.; Kruidhof, H.; Bouwmeester, Henricus J.M.; Burggraaf, Anthonie; Burggraaf, A.J.

    1997-01-01

    Oxygen permeation measurement is demonstrated, not only for a mixed oxide ionic and electronic conductor, but also as a new alternative to determine ambipolar conductivities, which can be usually reduced to be partial conductivities (either ionic or electronic). As a model system and an end member

  7. Experimental Validation of Hybrid Distillation-Vapor Permeation Process for Energy Efficient Ethanol-Water Separation

    Science.gov (United States)

    The energy demand of distillation-based systems for ethanol recovery and dehydration can be significant, particularly for dilute solutions. An alternative separation process integrating vapor stripping with a vapor compression step and a vapor permeation membrane separation step,...

  8. In Vitro-In Vivo Predictive Dissolution-Permeation-Absorption Dynamics of Highly Permeable Drug Extended-Release Tablets via Drug Dissolution/Absorption Simulating System and pH Alteration.

    Science.gov (United States)

    Li, Zi-Qiang; Tian, Shuang; Gu, Hui; Wu, Zeng-Guang; Nyagblordzro, Makafui; Feng, Guo; He, Xin

    2018-05-01

    Each of dissolution and permeation may be a rate-limiting factor in the absorption of oral drug delivery. But the current dissolution test rarely took into consideration of the permeation property. Drug dissolution/absorption simulating system (DDASS) valuably gave an insight into the combination of drug dissolution and permeation processes happening in human gastrointestinal tract. The simulated gastric/intestinal fluid of DDASS was improved in this study to realize the influence of dynamic pH change on the complete oral dosage form. To assess the effectiveness of DDASS, six high-permeability drugs were chosen as model drugs, including theophylline (pK a1  = 3.50, pK a2  = 8.60), diclofenac (pK a  = 4.15), isosorbide 5-mononitrate (pK a  = 7.00), sinomenine (pK a  = 7.98), alfuzosin (pK a  = 8.13), and metoprolol (pK a  = 9.70). A general elution and permeation relationship of their commercially available extended-release tablets was assessed as well as the relationship between the cumulative permeation and the apparent permeability. The correlations between DDASS elution and USP apparatus 2 (USP2) dissolution and also between DDASS permeation and beagle dog absorption were developed to estimate the predictability of DDASS. As a result, the common elution-dissolution relationship was established regardless of some variance in the characteristic behavior between DDASS and USP2 for drugs dependent on the pH for dissolution. Level A in vitro-in vivo correlation between DDASS permeation and dog absorption was developed for drugs with different pKa. The improved DDASS will be a promising tool to provide a screening method on the predictive dissolution-permeation-absorption dynamics of solid drug dosage forms in the early-phase formulation development.

  9. Thin film electronic devices with conductive and transparent gas and moisture permeation barriers

    Science.gov (United States)

    Simpson, Lin Jay

    2013-12-17

    A thin film stack (100, 200) is provided for use in electronic devices such as photovoltaic devices. The stack (100, 200) may be integrated with a substrate (110) such as a light transmitting/transmissive layer. A electrical conductor layer (120, 220) is formed on a surface of the substrate (110) or device layer such as a transparent conducting (TC) material layer (120,220) with pin holes or defects (224) caused by manufacturing. The stack (100) includes a thin film (130, 230) of metal that acts as a barrier for environmental contaminants (226, 228). The metal thin film (130,230) is deposited on the conductor layer (120, 220) and formed from a self-healing metal such as a metal that forms self-terminating oxides. A permeation plug or block (236) is formed in or adjacent to the thin film (130, 230) of metal at or proximate to the pin holes (224) to block further permeation of contaminants through the pin holes (224).

  10. The permeation of hydrogen in a steel at elevated temperature by an electrochemical method

    International Nuclear Information System (INIS)

    Tsubakino, H.; Ando, A.; Yamakawa, K.

    1984-01-01

    An electrochemical permeation method has been widely used to study the transport characteristics of hydrogen in metals, i.e., the content, diffusivity and permeability. This electrochemical measurement technique has the following remarkable features: a good detection limit for the measurement of hydrogen content, a simpler measuring apparatus, suitability for successive measurement of the transport characteristics, and flexibility in variation of experimental conditions. However this method has been restricted to temperatures below 373 K because an aqueous solution has been used as an electrode. In this study, an electrochemical permeation method using molten salts at elevated temperatures (673-773 K) in the range of practical interest for hydrogen attack in steel is presented. The introduction of hydrogen by use of molten salts has been reported but it has been performed at 423473 K and at 553 K

  11. Gas phase hydrogen permeation through ferritic iron, austenitic stainless steel and neutron irradiated austenitic stainless steel from near 3000K to 8730K

    International Nuclear Information System (INIS)

    Quick, N.R.

    1976-01-01

    Hydrogen permeation through iron was studied over the temperature range 300 to 873 0 K by an ultra high vacuum, monopole gas analyzer technique. Hydrogen gas input pressures were varied from 0.0043 to 0.62 atm and membrane thicknesses from 0.0165 to 0.243 cm. Volume diffusion control of the permeation process was demonstrated by the pressure and membrane thickness dependence of the steady state flux. The permeation coefficient, with an activation enthalpy found to be 8.1 +-.4 kcal/mole, was independent of both gas pressure and membrane thickness. At temperatures below approximately 600 0 K, the effective diffusivity increased with both increasing hydrogen gas pressure and increasing membrane thickness. The transition temperature from classical to anomalous behavior decreases with increasing thickness. Apparent activation enthalpies for diffusion were found to range from 1.6 to 8.2 kcal/mole with the lower values associated with thicker membranes. The permeation coefficient activation enthalpy was found to be 13.1 +- .4 kcal/mole while that for diffusivity was found to be 11.2 +- .45 kcal/mole. However, samples neutron irradiated at a fluence of 10 17 n/cm 2 showed anomalous effects in that both effective diffusivity and permeation were reduced in value

  12. Synergistic effect of iontophoresis and chemical enhancers on transdermal permeation of tolterodine tartrate for the treatment of overactive bladder

    Directory of Open Access Journals (Sweden)

    D. Prasanthi

    2013-01-01

    Full Text Available Purpose The objective of the study was to evaluate the synergistic transdermal permeation effect of chemical enhancers and iontophoresis technique on tolterodine tartrate (TT transdermal gel and to evaluate its pharmacokinetic properties. Materials and Methods Taguchi robust design was used for optimization of formulations. Skin permeation rates were evaluated using the Keshary-chein type diffusion cells in order to optimize the gel formulation. In-vivo studies of the optimized formulation were performed in a rabbit model and histopathology studies of optimized formulation were performed on rats. Results Transdermal gels were formulated successfully using Taguchi robust design method. The type of penetration enhancer, concentration of penetration enhancer, current density and pulse on/off ratio were chosen as independent variables. Type of penetration enhancer was found to be the significant factor for all the responses. Permeation parameters were evaluated when maximum cumulative amount permeated in 24 hours (Q24 was 145.71 ± 2.00µg/cm2 by CIT4 formulation over control (91.89 ± 2.30µg/cm2. Permeation was enhanced by 1.75 fold by CIT4 formulation. Formulation CIT4 containing nerolidol (5% and iontophoretic variables applied (0.5mA/cm2 and pulse on/off ratio 3:1 was optimized. In vivo studies with optimized formulation CIT4 showed increase in AUC and T1/2 when compared to oral suspension in rabbits. The histological studies showed changes in dermis indicating the effect of penetration enhancers and as iontophoresis was continued only for two cycles in periodic fashion so it did not cause any skin damage observed in the slides. Conclusion Results indicated that iontophoresis in combination with chemical enhancers is an effective method for transdermal administration of TT in the treatment of overactive bladder.

  13. Use of an in vitro human skin permeation assay to assess bioequivalence of two topical cream formulations containing butenafine hydrochloride (1%, w/w).

    Science.gov (United States)

    Mitra, Amitava; Kim, Nanhye; Spark, Darren; Toner, Frank; Craig, Susan; Roper, Clive; Meyer, Thomas A

    2016-12-01

    The primary objective of this work was to investigate, using an in vitro human skin permeation study, whether changes in the excipients of butenafine hydrochloride cream would have any effect on bioperformance of the formulation. Such in vitro data would be a surrogate for any requirement of a bioequivalence (BE) study to demonstrate formulation similarity. A LC-MS/MS method for quantitation of butenafine in various matrices was developed and validated. A pilot study was performed to validate the in vitro skin permeation methodology using three cream formulations containing butenafine hydrochloride at concentrations of 0.5, 1.0 and 1.5% (w/w). Finally, a definitive in vitro human skin permeation study was conducted, comparing the extent of butenafine hydrochloride permeation from the new formulation to that from the current formulation. The results of the study comparing the two formulations showed that there was no statistically significant difference in the extent of butenafine permeation into human skin. In conclusion, these in vitro data demonstrated that the formulation change is likely to have no significant impact on the bioperformance of 1% (w/w) butenafine hydrochloride cream. Copyright © 2016 Elsevier Inc. All rights reserved.

  14. Formulation of sage essential oil (Salvia officinalis, L.) monoterpenes into chitosan hydrogels and permeation study with GC-MS analysis.

    Science.gov (United States)

    Kodadová, Alexandra; Vitková, Zuzana; Herdová, Petra; Ťažký, Anton; Oremusová, Jarmila; Grančai, Daniel; Mikuš, Peter

    2015-01-01

    This study deals with the formulation of natural drugs into hydrogels. For the first time, compounds from the sage essential oil were formulated into chitosan hydrogels. A sample preparation procedure for hydrophobic volatile analytes present in a hydrophilic water matrix along with an analytical method based on the gas chromatography coupled with the mass spectrometry (GC-MS) was developed and applied for the evaluation of the identity and quantity of essential oil components in the hydrogels and saline samples. The experimental results revealed that the chitosan hydrogels are suitable for the formulation of sage essential oil. The monoterpene release can be effectively controlled by both chitosan and caffeine concentration in the hydrogels. Permeation experiment, based on a hydrogel with the optimized composition [3.5% (w/w) sage essential oil, 2.0% (w/w) caffeine, 2.5% (w/w) chitosan and 0.1% (w/w) Tween-80] in donor compartment, saline solution in acceptor compartment, and semi-permeable cellophane membrane, demonstrated the useful permeation selectivity. Here, (according to lipophilicity) an enhanced permeation of the bicyclic monoterpenes with antiflogistic and antiseptic properties (eucalyptol, camphor and borneol) and, at the same time, suppressed permeation of toxic thujone (not exceeding its permitted applicable concentration) was observed. These properties highlight the pharmaceutical importance of the developed chitosan hydrogel formulating sage essential oil in the dermal applications.

  15. Exploring natural variation of photosynthetic, primary metabolism and growth parameters in a large panel of Capsicum chinense accessions.

    Science.gov (United States)

    Rosado-Souza, Laise; Scossa, Federico; Chaves, Izabel S; Kleessen, Sabrina; Salvador, Luiz F D; Milagre, Jocimar C; Finger, Fernando; Bhering, Leonardo L; Sulpice, Ronan; Araújo, Wagner L; Nikoloski, Zoran; Fernie, Alisdair R; Nunes-Nesi, Adriano

    2015-09-01

    Collectively, the results presented improve upon the utility of an important genetic resource and attest to a complex genetic basis for differences in both leaf metabolism and fruit morphology between natural populations. Diversity of accessions within the same species provides an alternative method to identify physiological and metabolic traits that have large effects on growth regulation, biomass and fruit production. Here, we investigated physiological and metabolic traits as well as parameters related to plant growth and fruit production of 49 phenotypically diverse pepper accessions of Capsicum chinense grown ex situ under controlled conditions. Although single-trait analysis identified up to seven distinct groups of accessions, working with the whole data set by multivariate analyses allowed the separation of the 49 accessions in three clusters. Using all 23 measured parameters and data from the geographic origin for these accessions, positive correlations between the combined phenotypes and geographic origin were observed, supporting a robust pattern of isolation-by-distance. In addition, we found that fruit set was positively correlated with photosynthesis-related parameters, which, however, do not explain alone the differences in accession susceptibility to fruit abortion. Our results demonstrated that, although the accessions belong to the same species, they exhibit considerable natural intraspecific variation with respect to physiological and metabolic parameters, presenting diverse adaptation mechanisms and being a highly interesting source of information for plant breeders. This study also represents the first study combining photosynthetic, primary metabolism and growth parameters for Capsicum to date.

  16. Permeation Studies of CW Agents with Fabric Treated with 8-Hydroxyquinoline

    Science.gov (United States)

    2017-12-01

    Jeffery R. Owens AIR FORCE CIVIL ENGINEER CENTER AFCEC/CXAE, Tyndall AFB, FL 32403-5320 December 2017 Approved for public release: distribution...P.O. Box 68, Gunpowder, MD 21010-0068 Universal Technology Corp.; 139 Barnes Drive Suite 2 Tyndall AFB, FL 32404-5325 Air Force Civil Engineer ...moisture transport. The garment prevents exposure to outside chemical weapons agents (CWAs) by reacting or reducing permeation of the CWA. By

  17. Purification Simulation With Vapor Permeation and Distillation-Adsorption In Bioethanol Plant

    OpenAIRE

    Misri Gozan; Mia Sari Setiawan; Kenny Lischer

    2017-01-01

    High purity of Bioethanol is required in biofuel mixing with gasoline (EXX). In bioethanol production line, the azeotropic property of ethanol-water becomes the barrier for purification process. This study examined two bioethanol separation processes by support of simulation tools, Superpro Designer 9.0 software. Ethanol purity and a low costeconomical process were the major considerations. Purification method of vapor permeation membrane technology was compared with distillation-adsorption m...

  18. Electrically-controlled permeation of vapors through carbon nanotube network-based membranes

    Czech Academy of Sciences Publication Activity Database

    Slobodian, P.; Říha, Pavel; Olejník, R.

    2018-01-01

    Roč. 17, č. 2 (2018), s. 332-337 ISSN 1536-125X R&D Projects: GA MŠk ED2.1.00/19.0409 Grant - others:Ministerstvo školství, mládeže a tělovýchovy (MŠMT)(CZ) LO1504 Institutional research plan: CEZ:AV0Z20600510 Keywords : carbon nanotubes * electrically controlled membranes * permeation of chemical vapors Impact factor: 2.485, year: 2016

  19. Assessment of permeation quality of concrete through mercury intrusion porosimetry

    International Nuclear Information System (INIS)

    Kumar, Rakesh; Bhattacharjee, B.

    2004-01-01

    Permeation quality of laboratory cast concrete beams was determined through initial surface absorption test (ISAT). The pore system characteristics of the same concrete beam specimens were determined through mercury intrusion porosimetry (MIP). Data so obtained on the measured initial surface absorption rate of water by concrete and characteristics of pore system of concrete estimated from porosimetry results were used to develop correlations between them. Through these correlations, potential of MIP in assessing the durability quality of concrete in actual structure is demonstrated

  20. 4He permeation and H2O uptake of cyanate ester resins — an alternative to commonly used epoxy resins at low temperature

    Science.gov (United States)

    Nakamura, Sachiko; Fujii, Takenori; Matsukawa, Shoji; Katagiri, Masayuki; Fukuyama, Hiroshi

    2018-03-01

    Cyanate ester (CE) thermoset is a polymer with a high glass-transition temperature of ≈ 300 °C. CE is expected to be an alternative to Stycast 1266 as a sealing and casting glue for low temperature experiments, especially for adsorption experiments where baking of the substrate at T > 100 °C before cooling is required to eliminate surface contaminations. We experimentally confirmed that thermosets of CE monomers are non-porous and absorbs/desorbs water very little from measurements of (1) 4He permeation properties at temperatures from room temperature (RT) to 77 K and of (2) weight gains (δW) after storage for days in water and in air at RT. The 4He permeation is rather large at RT but negligibly small at T ⪅ 130 K where the diffusion constant of 4He in CE is vanishingly small. δW in water and air are 0.3–0.5% and 0.5–1.0%, respectively, which are much smaller than those of Stycast 1266. Therefore, cyanate ester is an excellent alternative to commonly used epoxy resins especially in surface-sensitive experiments at low temperature.

  1. Tritium permeation barrier based on self-healing composite materials

    Energy Technology Data Exchange (ETDEWEB)

    Gao Jifeng; Zhang Dan [State Key Laboratory of Mould Technology, Institute of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074 (China); Suo Jinping, E-mail: jpsuo@yahoo.com.cn [State Key Laboratory of Mould Technology, Institute of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074 (China)

    2010-12-15

    Pores and cracks in ceramic coatings is one of the most important problems to be solved for the thermally sprayed tritium permeation barriers (TPBs) in fusion reactor. In this work, we developed a self-healing composite coating to address this problem. The coating composed of TiC + mixture(TiC/Al{sub 2}O{sub 3}) + Al{sub 2}O{sub 3} was deposited on martensitic steels by means of atmospheric plasma spraying (APS). Before and after heat treatment, the morphology and phase of the coating were comparatively investigated by scanning electron microscopy (SEM) and X-ray diffraction (XRD). In the experiment, NiAl + Al{sub 2}O{sub 3}, mixture(TiC/Al{sub 2}O{sub 3}) + Al{sub 2}O{sub 3} and NiAl + TiC + mixture(TiC/Al{sub 2}O{sub 3}) + Al{sub 2}O{sub 3} films were also fabricated and studied, respectively. The results showed that the TiC + mixture(TiC/Al{sub 2}O{sub 3}) + Al{sub 2}O{sub 3} coating exhibited the best self-healing ability and good thermal shock resistance among the four samples after heat treatment under normal atmosphere. The SEM images analyzed by Image Pro software indicated that the porosity of the TiC + mixture(TiC/Al{sub 2}O{sub 3}) + Al{sub 2}O{sub 3} coating decreased more than 90% in comparison with the sample before heat treatment. This self-healing coating made by thermal spraying might be a good candidate for tritium permeation barrier in fusion reactors.

  2. Design, performance and manufacturing analysis for a compact permeator

    Energy Technology Data Exchange (ETDEWEB)

    Sacristan, R., E-mail: mrosa.sacristan@sener.es [SENER Ingenieria y Sistemas, Provenza 392, 5a 08025 Barcelona (Spain); Veredas, G. [EURATOM-CIEMAT Fusion Assoc., BBTU, Av. Complutense 22, 28040, Madrid (Spain); Bonjoch, I. [SENER Ingenieria y Sistemas, Provenza 392, 5a 08025 Barcelona (Spain); Fernandez, I. [EURATOM-CIEMAT Fusion Assoc., BBTU, Av. Complutense 22, 28040, Madrid (Spain); Martin, G. [SENER Ingenieria y Sistemas, Provenza 392, 5a 08025 Barcelona (Spain); Sanmarti, M. [b-FUS, Jardins de les Dones de Negre, 08930 Santa Adria de Besos, Barcelona (Spain); Sedano, L. [EURATOM-CIEMAT Fusion Assoc., BBTU, Av. Complutense 22, 28040, Madrid (Spain)

    2012-08-15

    Highlights: Black-Right-Pointing-Pointer Not a single manufacturing option fulfills fully all the requirements that the PAV needs due to complicated manufacturing geometry and materials. Black-Right-Pointing-Pointer To avoid permeation in piping joints is a challenge. Black-Right-Pointing-Pointer CFD sensibility analysis and efficiency analysis show that the aim of the experiment can be accomplished. - Abstract: A fast and efficient recovery of bred tritium is a major milestone of tritium breeding technologies R and D for the demonstration of a fusion reactor tritium self-sufficiency. Permeator against vacuum (PAV) runs as a single-step process for tritium on-line recovery, acts as passive systems allowing to be thermally governed can be easily in-pipe integrated in LiPb loop systems and can be conceived with high compactness. An optimal PAV design is proposed with detailed design parameterization of tritium recovery efficiency at different velocity ranges from numerical simulation based on properly developed Openfoam{sup Registered-Sign} CFD code BelFoam{sup Registered-Sign} customized solver. Diverse structural design options are being considered to manufacture the PAV component that presents diverse manufacturing concerns. Fabrication options are exploring rolling of thin plate with internal armor in the vacuum gap up to the coating of a porous controlled thick wall. The aim of this paper is to emphasize on the advanced performance of the PAV proposed concept, to present and discuss the different technical solutions that have been studied as well as the fabrication tests carried out.

  3. An assessment of the tritium inventory in, permeation through and releases from the NET plasma facing materials

    International Nuclear Information System (INIS)

    Wu, C.H.

    1986-01-01

    The tritium retention, permeation and release characteristics of D-T tokamaks are extremely important from both an environmental and a plasma physics point of view. Tokamak measurements have demonstrated that release of retained hydrogen isotopes by plasma-wall interactions play a dominant role in fuel recycling during a discharge. In addition, retained tritium in the plasma facing materials may contribute substantially to the on-site tritium inventory of D-T devices. Austenitic and martensitic steels are being considered as first wall materials. Tungsten and molybdenum will be possibly used as divertor armour materials for NET. By using a computer code, the tritium inventory in, permeation through and release from these materials have been calculated as functions of material thickness, temperature and impinging fluxes. It is shown that the tritium inventory in the first wall will be strongly affected by the temperature gradient in the materials. It is evident, that the tritium permeation as well as the tritium inventory can be reduced appropriately by controlling the temperatures at the plasma and cooling sides of the first wall. The results are discussed and the possible consequences are analysed. (author)

  4. On the intrinsic moisture permeation rate of remote microwave plasma-deposited silicon nitride layers

    NARCIS (Netherlands)

    van Assche, F. J. H.; Unnikrishnan, S.; Michels, J. J.; van Mol, A. M. B.; van de Weijer, P.; M. C. M. van de Sanden,; Creatore, M.

    2014-01-01

    We report on a low substrate temperature (110 °C) remote microwave plasma-enhanced chemical vapor deposition (PECVD) process of silicon nitride barrier layers against moisture permeation for organic light emitting diodes (OLEDs) and other moisture sensitive devices such as organic

  5. Analysis of the hydrogen permeation properties of TiN-TiC bilayers deposited on martensitic stainless steel

    International Nuclear Information System (INIS)

    Checchetto, R.; Horino, Y.; Benamati, G.

    1996-01-01

    The efficiency of TiN-TiC bilayer coatings, deposited by ion-beam-assisted deposition on martensitic steel, as a hydrogen permeation barrier was investigated by a gas phase method; the hydrogen permeability in the TiN-TiC bilayers is very low, at least 10 4 times lower than in the steel substrate in the temperature interval 470-570 K. Possible physical mechanisms, responsible for the reduced permeability of the ceramic bilayers, are discussed. In particular, from our experimental results, it can be concluded that chemisorption and/or hydrogen jumping from surface sites to the first subsurface atomic layer represents the hydrogen permeation limiting process. (orig.)

  6. Comprehensive metabolic characterization of serum osteocalcin action in a large non-diabetic sample

    DEFF Research Database (Denmark)

    Entenmann, Lukas; Pietzner, Maik; Artati, Anna

    2017-01-01

    to kynurenine points towards a pro-inflammatory state with increasing OCN. Inverse relations with intermediates of branch-chained amino acid metabolism suggest a link to energy metabolism. Finally, urinary surrogate markers of smoking highlight its adverse effect on OCN metabolism. In conclusion, the present...

  7. The JET gas baking plant for DT operation and analysis of tritium permeation and baking gas activation in DTE1

    Energy Technology Data Exchange (ETDEWEB)

    Pearce, R.J.H.; Andrew, P.; Bryan, S.; Hemmrich, J.L. [JET Joint Undertaking, Abingdon, Oxon (United Kingdom)

    1998-07-01

    The JET gas baking plant allows the vacuum vessel to be heated for conditioning and plasma operations. The vessel was maintained at 320 deg. C for the JET DT experiments (DTE 1). The design of the plant is outlined with particular reference to the features to provide compatibility with tritium operations. The experience of baking gas activation and tritium permeation into the plant are given, Developmentsto reduce the tritium permeation out of the vessel are considered. (authors)

  8. Properties of membranes to permeation to radon 222. New development for the measurement of radon 222 in water and water-saturated soils

    International Nuclear Information System (INIS)

    Labed, V.; Robe, M.C.

    1992-01-01

    Membranes that exclude water but are permeable to radon can extend the range of environments in which many radon detection systems could operate. We have studied the permeation of 222 Rn through membranes separating air and water phases. The permeation coefficients and the activation energy were calculated for various conditions. Potential applications such as in situ detection of radon in water are discussed

  9. Permeation of Light Gases through Hexagonal Ice

    Directory of Open Access Journals (Sweden)

    Luis Gales

    2012-09-01

    Full Text Available Gas separation using porous solids have attracted great attention due to their energetic applications. There is an enormous economic and environmental interest in the development of improved technologies for relevant processes, such as H2 production, CO2 separation or O2 and N2 purification from air. New materials are needed for achieving major improvements. Crystalline materials, displaying unidirectional and single-sized pores, preferentially with low pore tortuosity and high pore density, are promising candidates for membrane synthesis. Herein, we study hexagonal ice crystals as an example of this class of materials. By slowly growing ice crystals inside capillary tubes we were able to measure the permeation of several gas species through ice crystals and investigate its relation with both the size of the guest molecules and temperature of the crystal.

  10. Collagen-based silver nanoparticles: Study on cell viability, skin permeation, and swelling inhibition

    International Nuclear Information System (INIS)

    Saura Cardoso, Vinicius; Carvalho Filgueiras, Marcelo de; Medeiros Dutra, Yago; Gomes Teles, Ramon Handerson; Rodrigues de Araújo, Alyne; Primo, Fernando Lucas; Mafud, Ana Carolina; Batista, Larissa Fernandes; Mascarenhas, Yvonne Primerano

    2017-01-01

    Collagen is considered the most abundant protein in the animal kingdom, comprising 30% of the total amount of proteins and 6% of the human body by weight. Studies that examine the interaction between silver nanoparticles and proteins have been highlighted in the literature in order to understand the stability of the nanoparticle system, the effects observed in biological systems, and the appearance of new chemical pharmaceutical products. The objective of this study was to analyze the behavior of silver nanoparticles stabilized with collagen (AgNPcol) and to check the skin permeation capacity and action in paw edema induced by carrageenan. AgNPcol synthesis was carried out using solutions of reducing agent sodium borohydride (NaBH 4 ), silver nitrate (AgNO 3 ) and collagen. Characterization was done by using dynamic light scattering (DLS) and X-ray diffraction (XRD) and AFM. Cellular viability testing was performed by using flow cytometry in human melanoma cancer (MV3) and murine fibroblast (L929) cells. The skin permeation study was conducted using a Franz diffusion cell, and the efficiency of AgNPcol against the formation of paw edema in mice was evaluated. The hydrodynamic diameter and zeta potential of AgNPcol were 140.7 ± 7.8 nm and 20.1 ± 0.7 mV, respectively. AgNPcol failed to induce early apoptosis, late apoptosis, and necrosis in L929 cells; however, it exhibited enhanced toxicity in cancer cells (MV3) compared to normal cells (L929). AgNPcol demonstrated increased toxicological effects in cancer MV3 cells, promoting skin permeation, and preventing paw edema. - Highlights: • Silver nanoparticles were synthesized with type I collagen (AgNPcol). • AgNPcol which was characterized by XRD and DLS. • AgNPcol exhibited enhanced toxicity in cancer cells. • The efficiency of the AgNPcol against the paw edema was evaluated.

  11. Collagen-based silver nanoparticles: Study on cell viability, skin permeation, and swelling inhibition

    Energy Technology Data Exchange (ETDEWEB)

    Saura Cardoso, Vinicius, E-mail: vscfisio@ufpi.edu.br [Research Center in Biodiversity and Biotechnology, Biotec, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, 64202020 Parnaíba, Piauí (Brazil); Physiotherapy Department, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, 64202020 Parnaíba, Piauí (Brazil); Carvalho Filgueiras, Marcelo de; Medeiros Dutra, Yago; Gomes Teles, Ramon Handerson [Physiotherapy Department, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, 64202020 Parnaíba, Piauí (Brazil); Morphology and Muscle Physiology Laboratory, LAMFIM, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, 64202020 Parnaíba, Piauí (Brazil); Rodrigues de Araújo, Alyne [Research Center in Biodiversity and Biotechnology, Biotec, Campus Ministro Reis Velloso, Federal University of Piauí, UFPI, 64202020 Parnaíba, Piauí (Brazil); Primo, Fernando Lucas [Faculdade de Ciências Farmacêuticas, UNESP, Universidade Estadual Paulista, Campus de Araraquara, Departamento de Bioprocessos e Biotecnologia, 14800903 Araraquara, São Paulo (Brazil); Mafud, Ana Carolina; Batista, Larissa Fernandes; Mascarenhas, Yvonne Primerano [Institute of Physics of São Carlos, IFSC, University of São Paulo, USP, 13566590 São Carlos, SP (Brazil); and others

    2017-05-01

    Collagen is considered the most abundant protein in the animal kingdom, comprising 30% of the total amount of proteins and 6% of the human body by weight. Studies that examine the interaction between silver nanoparticles and proteins have been highlighted in the literature in order to understand the stability of the nanoparticle system, the effects observed in biological systems, and the appearance of new chemical pharmaceutical products. The objective of this study was to analyze the behavior of silver nanoparticles stabilized with collagen (AgNPcol) and to check the skin permeation capacity and action in paw edema induced by carrageenan. AgNPcol synthesis was carried out using solutions of reducing agent sodium borohydride (NaBH{sub 4}), silver nitrate (AgNO{sub 3}) and collagen. Characterization was done by using dynamic light scattering (DLS) and X-ray diffraction (XRD) and AFM. Cellular viability testing was performed by using flow cytometry in human melanoma cancer (MV3) and murine fibroblast (L929) cells. The skin permeation study was conducted using a Franz diffusion cell, and the efficiency of AgNPcol against the formation of paw edema in mice was evaluated. The hydrodynamic diameter and zeta potential of AgNPcol were 140.7 ± 7.8 nm and 20.1 ± 0.7 mV, respectively. AgNPcol failed to induce early apoptosis, late apoptosis, and necrosis in L929 cells; however, it exhibited enhanced toxicity in cancer cells (MV3) compared to normal cells (L929). AgNPcol demonstrated increased toxicological effects in cancer MV3 cells, promoting skin permeation, and preventing paw edema. - Highlights: • Silver nanoparticles were synthesized with type I collagen (AgNPcol). • AgNPcol which was characterized by XRD and DLS. • AgNPcol exhibited enhanced toxicity in cancer cells. • The efficiency of the AgNPcol against the paw edema was evaluated.

  12. Vapor Permeation and Pervaporation of Propan- 1-ol and Propan- 2-ol in Polyethylene Membrane

    Czech Academy of Sciences Publication Activity Database

    Uchytil, Petr; Petričkovič, Roman

    2002-01-01

    Roč. 209, - (2002), s. 67-79 ISSN 0376-7388 R&D Projects: GA ČR GA104/01/0945 Keywords : vapor permeation * pervaporation * sorption Subject RIV: CI - Industrial Chemistry, Chemical Engineering Impact factor: 1.965, year: 2002

  13. Corrosion behaviour of Al based tritium permeation barriers in flowing Pb-17Li

    International Nuclear Information System (INIS)

    Glasbrenner, H.; Konys, J.; Voss, Z.; Wedemeyer, O.

    2002-01-01

    Tritium permeation barriers on low-activation steels are required in fusion technology in order to reduce the tritium permeation rate through the structural material into the cooling water system. Al-Fe layers with alumina on top can fulfil the required reduction rate. Three techniques were selected to produce such a multi-layered coating system: chemical vapour deposition (CVD) by CEA, hot-dip aluminising (HDA) by FZK and vacuum plasma spraying (VPS) by JRC Ispra. A sufficient corrosion resistance against Pb-17Li attack is also required for the coating. Therefore, the corrosion behaviour of these three coatings on ferritic-martensitic steels was studied in the PICOLO loop of FZK in flowing Pb-17Li at 480 deg. C up to 10 000 h. Corrosion effects could not be found on HDA and VPS coated specimens even up to the longest time of exposure. The total thickness of the two-layered system remained unchanged at around 130 μm for all examined HDA and VPS specimens. In contrast to this, corrosion effects could be inspected on CVD coated specimens

  14. Effect of Different Skin Penetration Promoters in Halobetasol Propionate Permeation and Retention in Human Skin

    Directory of Open Access Journals (Sweden)

    Paulina Carvajal-Vidal

    2017-11-01

    Full Text Available Halobetasol propionate (HB is a potent synthetic corticosteroid used against inflammatory skin diseases, such as dermatitis, eczema, and psoriasis, among others. The aim of this study is to define how the presence of different skin penetration enhancers (nonane, menthone, limonene, azone, carene, decanol, linoleic acid and cetiol affects the penetration and retention in skin of HB. To determine drug penetration through skin, 5% of each promoter was used in an ex vivo system with human skin on Franz cells. The results showed that the highest permeation occurs in the presence of menthone, followed by nonane. Permeation parameters were determined. The in vivo test was assessed, and the formulation containing HB-menthone presented better anti-inflammatory efficacy. These results are useful to generate a specific treatment according to each patient’s needs, and the inflammatory characteristics of the disease.

  15. A study of radon 222 permeation through plastic membranes. Application to a method of radon measurement in water and saturated soils

    International Nuclear Information System (INIS)

    Labed, V.

    1991-04-01

    In order to improve the BARASOL R device and to use it in water-saturated soils and in pressure constraint conditions, we have studied radon 222 permeation through plastic membranes. While the permeation process usually takes place between two media being in the same state, most often gaseous, the present study describes the transfer of radon 222 from the water to the air via a membrane. Polypropylene membranes have been tested with an experimental set-up by monitoring the evolution of radon concentrations in water and in air. The permeation coefficient and the activation energy were calculated in various conditions. With a second experimental set-up, we have tested the polyethylene membrane which has been adapted on the BARASOL. In these conditions, we have shown that it is possible to measure radon in water at concentrations around 10 3 Bq.m -3 [fr

  16. Toward enhanced hydrogen generation from water using oxygen permeating LCF membranes

    KAUST Repository

    Wu, Xiao-Yu

    2015-01-01

    © the Owner Societies. Hydrogen production from water thermolysis can be enhanced by the use of perovskite-type mixed ionic and electronic conducting (MIEC) membranes, through which oxygen permeation is driven by a chemical potential gradient. In this work, water thermolysis experiments were performed using 0.9 mm thick La0.9Ca0.1FeO3-δ (LCF-91) perovskite membranes at 990 °C in a lab-scale button-cell reactor. We examined the effects of the operating conditions such as the gas species concentrations and flow rates on the feed and sweep sides on the water thermolysis rate and oxygen flux. A single step reaction mechanism is proposed for surface reactions, and three-resistance permeation models are derived. Results show that water thermolysis is facilitated by the LCF-91 membrane especially when a fuel is added to the sweep gas. Increasing the gas flow rate and water concentration on the feed side or the hydrogen concentration on the sweep side enhances the hydrogen production rate. In this work, hydrogen is used as the fuel by construction, so that a single-step surface reaction mechanism can be developed and water thermolysis rate parameters can be derived. Both surface reaction rate parameters for oxygen incorporation/dissociation and hydrogen-oxygen reactions are fitted at 990 °C. We compare the oxygen fluxes in water thermolysis and air separation experiments, and identify different limiting steps in the processes involving various oxygen sources and sweep gases for this 0.9 mm thick LCF-91 membrane. In the air feed-inert sweep case, the bulk diffusion and sweep side surface reaction are the two limiting steps. In the water feed-inert sweep case, surface reaction on the feed side dominates the oxygen permeation process. Yet in the water feed-fuel sweep case, surface reactions on both the feed and sweep sides are rate determining when hydrogen concentration in the sweep side is in the range of 1-5 vol%. Furthermore, long term studies show that the surface

  17. Multi-Dielectric Brownian Dynamics and Design-Space-Exploration Studies of Permeation in Ion Channels.

    Science.gov (United States)

    Siksik, May; Krishnamurthy, Vikram

    2017-09-01

    This paper proposes a multi-dielectric Brownian dynamics simulation framework for design-space-exploration (DSE) studies of ion-channel permeation. The goal of such DSE studies is to estimate the channel modeling-parameters that minimize the mean-squared error between the simulated and expected "permeation characteristics." To address this computational challenge, we use a methodology based on statistical inference that utilizes the knowledge of channel structure to prune the design space. We demonstrate the proposed framework and DSE methodology using a case study based on the KcsA ion channel, in which the design space is successfully reduced from a 6-D space to a 2-D space. Our results show that the channel dielectric map computed using the framework matches with that computed directly using molecular dynamics with an error of 7%. Finally, the scalability and resolution of the model used are explored, and it is shown that the memory requirements needed for DSE remain constant as the number of parameters (degree of heterogeneity) increases.

  18. Absence of molecular deuterium dissociation during room-temperature permeation into polystyrene ICF target shells

    International Nuclear Information System (INIS)

    Honig, A.; Alexander, N.; Fan, Q.; Gram, R.; Kim, H.

    1991-01-01

    Polystyrene microshells filled with deuterium and tritium gas are important target shells for inertially confined fusion (ICF) and are particularly promising for target containing spin-polarized hydrogens fuels. A currently active approach to the latter uses polarized D in HD, in a method which requires preservation of the high purity of the initially prepared HD (very low specified H 2 and D 2 concentrations). This would not be possible if dissociation should occur during permeation into the target shells. We have thus tested polystyrene shells using a novel method which employs very pure polystyrene shells using a novel method which employs very pure ortho-D 2 as the test gas. An upper limit of 6 x 10 -4 was deduced for the dissociation of D 2 upon room temperature permeation through an approximately 8 um wall of polystyrene, clearing the way for use of polystyrene target shells for ICF fusion experiments with spin-polarized hydrogens fuels. 19 refs., 1 fig

  19. Study on low level radioactive wastewater treatment by inorganic membrane permeation combined with complexation

    International Nuclear Information System (INIS)

    Li Junfeng; Wang Jianlong; Bai Qinzhong

    2007-01-01

    Inorganic membranes exhibit greater mechanical durability in some operations than polymeric membranes. They do not suffer from the performance degradation that was resulted from compaction of the membrane structure under pressure or ageing. Membrane permeation combined with complexation was tested for radioactive wastes processing purpose. Sodium poly-acrylic acid was selected as the complexing agent, the efficiency of inorganic membrane with cut-off 1kD, 3kD, 8kD assisted by sodium poly-acrylic acid of different molecular weight were compared. The removal efficiencies of nuclides such as strontium, cesium and cobalt by were compared. The flux and retention factors of different membrane system were compared. The impacts of complexation agent concentration on permeate flux retention factors were studied. The long term behaviours of the membrane system were also studied. Diatomite filter was selected as the pretreatment method, and the efficiency of diatomite filter for pretreatment was investigated also. (author)

  20. Hydrogen permeation on Al{sub 2}O{sub 3}-based nickel/cobalt composite membranes

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jihee; Jung, Miewon [Department of Chemistry/Institute of Basic Science, Sungshin Women' s University, Seoul 136-742 (Korea, Republic of); Hong, Tae-Whan [Department of Materials Science and Engineering/Research Center for Sustainable Eco-Devices and Materials(ReSEM), Chungju National University, Chungju 380-702 (Korea, Republic of)

    2010-12-15

    Al{sub 2}O{sub 3} was synthesized using the sol-gel process with aluminum isopropoxide as the precursor and primary distilled water as the solvent. Nickel and cobalt metal powders were used to increase the strength of the membranes. The Al{sub 2}O{sub 3}-based membranes were prepared using HPS following a mechanical alloying process. The phase transformation, thermal evolution, surface and cross-section morphology of Al{sub 2}O{sub 3} and Al{sub 2}O{sub 3}-based membranes were characterized by XRD, TG-DTA and FE-SEM. The hydrogen permeation of Al{sub 2}O{sub 3}-based membranes was examined at 300-473 K under increasing pressure. Hydrogen permeation flux through an Al{sub 2}O{sub 3}-20wt%Co membrane was obtained to 2.36 mol m{sup -2} s{sup -1}. Reaction enthalpy was calculated to 4.5 kJ/mol using a Van't Hoff's plot. (author)

  1. Optical coherence tomography in quantifying the permeation of human plasma lipoproteins in vascular tissues

    Science.gov (United States)

    Ghosn, M. G.; Mashiatulla, M.; Tuchin, V. V.; Morrisett, J. D.; Larin, K. V.

    2012-03-01

    Atherosclerosis is the most common underlying cause of vascular disease, occurring in multiple arterial beds including the carotid, coronary, and femoral arteries. Atherosclerosis is an inflammatory process occurring in arterial tissue, involving the subintimal accumulation of low-density lipoproteins (LDL). Little is known about the rates at which these accumulations occur. Measurements of the permeability rate of LDL, and other lipoproteins such as high-density lipoprotein (HDL) and very low-density lipoprotein (VLDL), could help gain a better understanding of the mechanisms involved in the development of atherosclerotic lesions. The permeation of VLDL, LDL, HDL, and glucose was monitored and quantified in normal and diseased human carotid endarterectomy tissues at 20°C and 37°C using optical coherence tomography (OCT). The rates for LDL permeation through normal tissue at 20°C was (3.16 +/- 0.37) × 10-5 cm/sec and at 37°C was (4.77 +/- 0.48) × 10-5 cm/sec, significantly greater (plipoproteins.

  2. S-protected thiolated chitosan for oral delivery of hydrophilic macromolecules: evaluation of permeation enhancing and efflux pump inhibitory properties.

    Science.gov (United States)

    Dünnhaupt, Sarah; Barthelmes, Jan; Rahmat, Deni; Leithner, Katharina; Thurner, Clemens C; Friedl, Heike; Bernkop-Schnürch, Andreas

    2012-05-07

    The objective of this study was the investigation of permeation enhancing and P-glycoprotein (P-gp) inhibition effects of a novel thiolated chitosan, the so-named S-protected thiolated chitosan. Mediated by a carbodiimide, increasing amounts of thioglycolic acid (TGA) were covalently bound to chitosan (CS) in the first step of modification. In the second step, these thiol groups of thiolated chitosan were protected by disulfide bond formation with the thiolated aromatic residue 6-mercaptonicotinamide (6-MNA). Mucoadhesive properties of all conjugates were evaluated in vitro on porcine intestinal mucosa based on tensile strength investigations. Permeation enhancing effects were evaluated ex vivo using rat intestinal mucosa and in vitro via Caco-2 cells using the hydrophilic macromolecule FD(4) as the model drug. Caco-2 cells were further used to show P-gp inhibition effects by using Rho-123 as P-gp substrate. Apparent permeability coefficients (P(app)) were calculated and compared to values obtained from each buffer control. Three different thiolated chitosans were generated in the first step of modification, which displayed increasing amounts of covalently attached free thiol groups on the polymer backbone. In the second modification step, more than 50% of these free thiol groups were covalently linked with 6-MNA. Within 3 h of permeation studies on excised rat intestine, P(app) values of all S-protected chitosans were at least 1.3-fold higher compared to those of corresponding thiomers and more than twice as high as that of unmodified chitosan. Additional permeation studies on Caco-2 cells confirmed these results. Because of the chemical modification and higher amount of reactive thiol groups, all S-protected thiolated chitosans exhibit at least 1.4-fold pronounced P-gp inhibition effects in contrast to their corresponding thiomers. These features approve S-protected thiolated chitosan as a promising excipient for various drug delivery systems providing improved

  3. Pluronic lecithin organogel (PLO) of diltiazem hydrochloride: effect of solvents/penetration enhancers on ex vivo permeation.

    Science.gov (United States)

    Parhi, Rabinarayan; Suresh, Podilam; Pattnaik, Subasini

    2016-06-01

    In the present study, pluronic lecithin organogel (PLO) of diltiazem hydrochloride (DZH) was developed by taking different ratios of organic phase to aqueous phase (1:3, 1:4, and 1:5) with varying concentration of soya lecithin (20, 30, and 40 % w/w) in organic phase (isopropyl myristate, IPM) and pluronic (20, 25, and 30 % w/w) in aqueous phase, respectively, and characterized for in vitro parameters and ex vivo permeation study. The results of in vitro parameters were found to be within permissible limit and all the PLOs were physically stable at refrigeration and ambient temperature. The influence of phase ratio and different concentrations of soya lecithin on DZH release from the PLOs was found to be significant (p < 0.05), whereas the influences of different concentrations of pluronic were insignificant. The effect of different solvents/penetration enhancers viz. IPM, propylene glycol (PG), dimethyl sulphoxide (DMSO), and D-limonene, in combination and alone, on the permeation of DZH across the dorsal skin of rat was studied. Among all, formulation containing IPM (PLO6) exhibited highest flux of 147.317 μg/cm(2)/h. Furthermore, histopathology section of treated skin sample illustrated that lipid bilayer disruption was the mechanism for the DZH permeation. The above results indicated that PLO6 may serve as a promising alternative delivery system for DZH in the effective treatment of hypertension.

  4. Reversed phase parallel artificial membrane permeation assay for log P measurement

    Directory of Open Access Journals (Sweden)

    Zihao Song

    2016-03-01

    Full Text Available A reversed phase parallel artificial membrane permeation assay (RP-PAMPA was newly invented for log P measurement. An oil/water/oil sandwich was constructed using a conventional PAMPA instrument. 1 % agarose was used to improve the physical stability of the water phase. A linear correlation between log P and the apparent permeability was observed in the -0.24 < log P < 2.85 region (R2 = 0.98. RP-PAMPA was also applied to pKa measurement.

  5. Influence of He ions irradiation on the deuterium permeation and retention behavior in the CLF-1 steel

    International Nuclear Information System (INIS)

    Xu, Yu-Ping; Lu, Tao; Li, Xiao-Chun; Liu, Feng; Liu, Hao-Dong; Wang, Jing; An, Zhong-Qing; Ding, Fang; Hong, Suk-Ho; Zhou, Hai-Shan; Luo, Guang-Nan

    2016-01-01

    To evaluate the influence of He ions irradiation on the deuterium permeation and retention behavior in RAFM steels, samples made of the CLF-1 steel was irradiated with 3.5 MeV He ions. Gas driven permeation experiments were performed, and the permeability of virgin sample and pre-irradiated sample were obtained and compared. In order to characterize the effect of He ions irradiation on the deuterium retention behavior, deuterium gas exposure was carried out at 623 K, followed by thermal desorption spectra experiments. The total deuterium retention of the CLF-1 steel increased owing to He ions implantation, which could be attributed to the increase in trapping site for deuterium by the He pre-irradiation.

  6. Lidocaine self-sacrificially improves the skin permeation of the acidic and poorly water-soluble drug etodolac via its transformation into an ionic liquid.

    Science.gov (United States)

    Miwa, Yasushi; Hamamoto, Hidetoshi; Ishida, Tatsuhiro

    2016-05-01

    Poor transdermal penetration of active pharmaceutical ingredients (APIs) impairs both bioavailability and therapeutic benefits and is a major challenge in the development of transdermal drug delivery systems. Here, we transformed a poorly water-soluble drug, etodolac, into an ionic liquid in order to improve its hydrophobicity, hydrophilicity and skin permeability. The ionic liquid was prepared by mixing etodolac with lidocaine (1:1, mol/mol). Both the free drug and the transformed ionic liquid were characterized by differential scanning colorimetry (DSC), infrared spectroscopy (IR), and saturation concentration measurements. In addition, in vitro skin-permeation testing was carried out via an ionic liquid-containing patch (Etoreat patch). The lidocaine and etodolac in ionic liquid form led to a relatively lower melting point than either lidocaine or etodolac alone, and this improved the lipophilicity/hydrophilicity of etodolac. In vitro skin-permeation testing demonstrated that the Etoreat patch significantly increased the skin permeation of etodolac (9.3-fold) compared with an etodolac alone patch, although an Etoreat patch did not increase the skin permeation of lidocaine, which was consistent with the results when using a lidocaine alone patch. Lidocaine appeared to self-sacrificially improve the skin permeation of etodolac via its transformation into an ionic liquid. The data suggest that ionic liquids composed of approved drugs may substantially expand the formulation preparation method to meet the challenges of drugs which are characterized by poor rates of transdermal absorption. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

  7. Studies on oxidation and deuterium permeation behavior of a low temperature α-Al_2O_3-forming Fe−Cr−Al ferritic steel

    International Nuclear Information System (INIS)

    Xu, Yu-Ping; Zhao, Si-Xiang; Liu, Feng; Li, Xiao-Chun; Zhao, Ming-Zhong; Wang, Jing; Lu, Tao; Hong, Suk-Ho; Zhou, Hai-Shan; Luo, Guang-Nan

    2016-01-01

    To evaluate the capability of Fe−Cr−Al ferritic steels as tritium permeation barrier in fusion systems, the oxidation behavior together with the permeation behavior of a Fe−Cr−Al steel was investigated. Gas driven permeation experiments were performed. The permeability of the oxidized Fe−Cr−Al steel was obtained and a reduced activation ferritic/martensitic steel CLF-1 was used as a comparison. In order to characterize the oxide layer, SEM, XPS, TEM, HRTEM were used. Al_2O_3 was detected in the oxide film by XPS, and HRTEM showed that Al_2O_3 in the α phase was found. The formation of α-Al_2O_3 layer at a relatively low temperature may result from the formation of Cr_2O_3 nuclei.

  8. Effect of chemical permeation enhancers on stratum corneum barrier lipid organizational structure and interferon alpha permeability.

    Science.gov (United States)

    Moghadam, Shadi H; Saliaj, Evi; Wettig, Shawn D; Dong, Chilbert; Ivanova, Marina V; Huzil, J Torin; Foldvari, Marianna

    2013-06-03

    The outermost layer of the skin, known as the stratum corneum (SC), is composed of dead corneocytes embedded in an intercellular lipid matrix consisting of ceramides, free fatty acids, and cholesterol. The high level of organization within this matrix protects the body by limiting the permeation of most compounds through the skin. While essential for its protective functions, the SC poses a significant barrier for the delivery of topically applied pharmaceutical agents. Chemical permeation enhancers (CPEs) can increase delivery of small drug compounds into the skin by interacting with the intercellular lipids through physical processes including extraction, fluidization, increased disorder, and phase separation. However, it is not clear whether these same mechanisms are involved in delivery of biotherapeutic macromolecules, such as proteins. Here we describe the effect of three categories of CPEs {solvents [ethanol, propylene glycol, diethylene glycol monoethyl ether (transcutol), oleic acid], terpenes [menthol, nerol, camphor, methyl salicylate], and surfactants [Tween 80, SDS, benzalkonium chloride, polyoxyl 40 hydrogenated castor oil (Cremophor RH40), didecyldimethylammonium bromide (DDAB), didecyltrimethylammonium bromide (DTAB)]} on the lipid organizational structure of human SC as determined by X-ray scattering studies. Small- and wide-angle X-ray scattering studies were conducted to correlate the degree of structural changes and hydrocarbon chain packing in SC lipids caused by these various classes of CPEs to the extent of permeation of interferon alpha-2b (IFNα), a 19 kDa protein drug, into human skin. With the exception of solvents, propylene glycol and ethanol, all classes of CPEs caused increased disordering of lamellar and lateral packing of lipids. We observed that the highest degree of SC lipid disordering was caused by surfactants (especially SDS, DDAB, and DTAB) followed by terpenes, such as nerol. Interestingly, in vitro skin permeation studies

  9. Fabrication of lipidic nanocarriers of loratadine for facilitated intestinal permeation using multivariate design approach.

    Science.gov (United States)

    Verma, Samridhi; Singh, Sandeep Kumar; Verma, Priya Ranjan Prasad

    2016-01-01

    In this investigation, multivariate design approach was employed to develop self-nanoemulsifying drug delivery system (SNEDDS) of loratadine and to exploit its potential for intestinal permeability. Drug solubility was determined in various vehicles and existence of self-nanoemulsifying region was evaluated by phase diagram studies. The influence of formulation variables X1 (Capmul MCM C8) and X2 (Solutol HS15) on SNEDDS was assessed for mean globule sizes in different media (Y1-Y3), emulsification time (Y4) and drug-release parameters (Y5-Y6), to improve quality attributes of SNEDDS. Significant models were generated, statistically analyzed by analysis of variance and validated using the residual and leverage plots. The interaction, contour and response plots explicitly demonstrated the influence of one factor on the other and displayed trend of factor-effect on responses. The pH-independent optimized formulation was obtained with appreciable global desirability (0.9266). The strenuous act of determining emulsification time is innovatively replaced by the use of oil-soluble dye to produce visibly distinct globules that otherwise may be deceiving. TEM images displayed non-aggregated state of spherical globules (size < 25 nm) and also revealed the structural transitions occurring during emulsification. Optimized formulation exhibited non-Newtonian flow justified by the model-fit and also presented the stability to dilution effects and thermodynamic stress testing. The ex vivo permeation study using confocal laser scanning microscopy indicate strong potential of rhodamine 123-loaded loratadine-SNEDDS to inhibit P-gp efflux and facilitate intestinal permeation. To conclude, the effectiveness of design yields a stable optimized SNEDDS with enhanced permeation potential, which is expected to improve oral bioavailability of loratadine.

  10. Oxygen permeation and thermo-chemical stability of oxygen separation membrane materials for the oxyfuel process

    Energy Technology Data Exchange (ETDEWEB)

    Ellett, Anna Judith

    2009-07-01

    The reduction of CO{sub 2} emissions, generally held to be one of the most significant contributors to global warming, is a major technological issue. CO{sub 2} Capture and Storage (CCS) techniques applied to large stationary sources such as coal-fired power plants could efficiently contribute to the global carbon mitigation effort. The oxyfuel process, which consists in the burning of coal in an oxygen-rich atmosphere to produce a flue gas highly concentrated in CO{sub 2}, is a technology considered for zero CO{sub 2} emission coal-fired power plants. The production of this O{sub 2}-rich combustion gas from air can be carried out using high purity oxygen separation membranes. Some of the most promising materials for this application are mixed ionic-electronic conducting (MIEC) materials with perovskite and K{sub 2}NiF{sub 4} perovskite-related structures. The present work examines the selection of La{sub 0.58}Sr{sub 0.4}Co{sub 0.2}Fe{sub 0.8}O{sub 3-{delta}} (LSCF58), La{sub 2}NiO{sub 4+{delta}}, Pr{sub 0.58}Sr{sub 0.4}Co{sub 0.2}Fe{sub 0.8}O{sub 3-{delta}} (PSCF58) and Ba{sub 0.5}Sr{sub 0.5}Co{sub 0.8}Fe{sub 0.2}O{sub 3-{delta}} (BSCF50) as membrane materials for the separation of O{sub 2} and N{sub 2} in the framework of the oxyfuel process with flue gas recycling. Annealing experiments were carried out on pellets exposed to CO{sub 2}, water vapour, O{sub 2} and Cr{sub 2}O{sub 3} in order to determine the thermo-chemical resistance to the atmospheres and the high temperature conditions present during membrane operation in a coal-fired power plant. The degradation of their microstructure was investigated using Scanning Electron Microscopy (SEM) in combination with electron dispersive spectroscopy (EDS) as well as X-Ray Diffraction (XRD). Also, the oxygen permeation fluxes of selected membranes were investigated as a function of temperature. The membrane materials selected were characterised using thermo-analytical techniques such as precision thermogravimetric

  11. Comparison of the effect of fatty alcohols on the permeation of melatonin between porcine and human skin.

    Science.gov (United States)

    Andega, S; Kanikkannan, N; Singh, M

    2001-11-09

    Melatonin (MT) is a hormone secreted by the pineal gland that plays an important role in the regulation of the circadian sleep-wake cycle. It would be advantageous to administer MT using a transdermal delivery system for the treatment of sleep disorders such as delayed sleep syndrome, jet lag in travelers, cosmonauts and shift workers. The porcine skin has been found to have similar morphological and functional characteristics as human skin. The elastic fibres in the dermis, enzyme pattern of the epidermis, epidermal tissue turnover time, keratinous proteins and thickness of epidermis of porcine skin are similar to human skin. However, the fat deposition and vascularisation of the cutaneous glands of porcine skin are different from human skin. In addition, porcine skin has been found to have a close permeability character to human skin. However, the comparative effect of chemical penetration enhancers on the permeation of drugs between porcine and human skin has not been reported. The purpose of this study was to compare the effect of fatty alcohols on the permeability of porcine and human skin using MT as a model compound. The effect of saturated fatty alcohols (octanol, nonanol, decanol, undecanol, lauryl alcohol, tridecanol, myristyl alcohol) and unsaturated fatty alcohols (oleyl alcohol, linoleyl alcohol, linolenyl alcohol) at 5% concentration was tested across dermatomed porcine and human skin. Our studies showed a parabolic relationship between the carbon chain length of saturated fatty alcohols and permeation enhancement of MT with both porcine and human skin. Maximum permeation of MT was observed when fatty alcohol carbon chain length was 10. In general, as the level of unsaturation increased from one to two double bonds, there was an increase in the permeation of MT both in porcine and human skin. However, a decrease in the permeation was observed with three double bonds. Regression analysis using the steady state flux data showed a significant positive

  12. An experimental device for measurement of gas permeation in solid matrices

    International Nuclear Information System (INIS)

    De Salve, M.; Mazzi, E.; Zucchetti, M.

    1996-01-01

    The inventory in and the permeation through fusion reactor structures of hydrogen and its isotopes play an important role in the machine operation, evolution of material properties, and safety. An experimental and research activity for the determination of permeability (and derived parameters) of gases in solid matrices is described. It uses a gas permeation method, that basically consists in the measure of the time evolution of the gas pressure in a chamber in which vacuum has been previously made (downstream volume). This chamber is separated from another one, full of the gas in exam (upstream volume), by means of a membrane of the material under study. The experimental installation is described. The first stage of the experimental activity has dealt with the set-up of the device, the volume calibration, and the definition of the parameters range for which the installation can give reliable measurements. The subsequent stage of the activity has consisted in the measurement of the permeability, and then of the diffusion coefficient, of nitrogen in some materials at room temperature. Concurrently with the experimental activity, a model has been set-up and implemented in a computer code: this code permits to evaluate the time evolution of the pressure in the downstream chamber. With this code, using the measured parameters, the time evolution of the pressure experimentally measured has been satisfactorily reproduced. (author)

  13. Resistance of gloves and protective clothing materials to permeation of cytostatic solutions

    OpenAIRE

    Sylwia Krzemińska; Małgorzata Pośniak; Małgorzata Szewczyńska

    2018-01-01

    Objectives The objective of the work was to determine the resistance of selected protective clothing and glove materials to permeation of cytostatics such as docetaxel, fluorouracil, and doxorubicin. Material and Methods The following glove materials were used: natural rubber latex (code A), acrylonitrile-butadiene rubber (code B) and chloroprene rubber (code C). In addition, we tested a layered material composed of a non-woven polyester (PES), a polypropylene (PP) film, ...

  14. Physical Aging, Plasticization and Their Effects on Gas Permeation in “Rigid” Polymers of Intrinsic Microporosity

    KAUST Repository

    Swaidan, Raja; Ghanem, Bader; Litwiller, Eric; Pinnau, Ingo

    2015-01-01

    Long-term physical aging and plasticization, two mobility-based phenomena that are counterintuitive in the context of “rigid” polymers of intrinsic microporosity (PIMs), were evaluated using pure- and mixed-gas permeation data for representative

  15. STRUCTURAL INTERACTIONS OF HYDROGEN WITH BULK AMORPHOUS MICROSTRUCTURES IN METALLIC SYSTEMS UNDERSTANDING THE ROLE OF PARTIAL CRYSTALLINITY ON PERMEATION AND EMBRITTLEMENT

    Energy Technology Data Exchange (ETDEWEB)

    Brinkman, Kyle; Fox, Elise; Korinko, Paul; Adams, Thad

    2010-05-10

    The development of metallic glasses in bulk form has led to a resurgence of interest into the utilization of these materials for a variety of applications. A potentially exciting application for these bulk metallic glass (BMG) materials is their use as composite membranes to replace high cost Pd/Pd-alloy membranes for enhanced gas separation processes. One of the major drawbacks to the industrial use of Pd/Pd-alloy membranes is that during cycling above and below a critical temperature an irreversible change takes place in the palladium lattice structure which can result in significant damage to the membrane. Furthermore, the cost associated with Pd-based membranes is a potential detractor for their continued use and BMG alloys offer a potentially attractive alternative. Several BMG alloys have been shown to possess high permeation rates, comparable to those measured for pure Pd metal. In addition, high strength and toughness when either in-situ or ex-situ second phase dispersoids are present. Both of these properties, high permeation and high strength/toughness, potentially make these materials attractive for gas separation membranes that could resist hydrogen 'embrittlement'. However, a fundamental understanding of the relationship between partially crystalline 'structure'/devitrification and permeation/embrittlement in these BMG materials is required in order to determine the operating window for separation membranes and provide additional input to the material synthesis community for improved alloy design. This project aims to fill the knowledge gap regarding the impact of crystallization on the permeation properties of metallic glass materials. The objectives of this study are to (i) determine the crystallization behavior in different gas environments of Fe and Zr based commercially available bulk metallic glass and (ii) quantify the effects of partial crystallinity on the hydrogen permeation properties of these metallic glass membranes.

  16. Pretherapy metabolic tumour volume is an independent predictor of outcome in patients with diffuse large B-cell lymphoma

    Energy Technology Data Exchange (ETDEWEB)

    Sasanelli, Myriam; Meignan, Michel; Haioun, Corinne; Itti, Emmanuel [Paris-Est University, Nuclear Medicine and Lymphoid Malignancies Unit, Henri Mondor Hospital, Creteil (France); Berriolo-Riedinger, Alina; Casasnovas, Rene-Olivier [Nuclear Medicine and Hematology, Georges-Francois Leclerc Center, Le Bocage Hospital, Dijon (France); Biggi, Alberto; Gallamini, Andrea [Nuclear Medicine and Hematology, Santa Croce e Carle Hospital, Cuneo (Italy); Siegel, Barry A.; Cashen, Amanda F. [Washington University School of Medicine, Nuclear Medicine and Oncology, Siteman Cancer Center, St. Louis, MO (United States); Vera, Pierre; Tilly, Herve [Nuclear Medicine and Hematology, Henri Becquerel Center, Rouen (France); Versari, Annibale [Nuclear Medicine, Santa Maria Nuova Hospital-IRCCS, Reggio Emilia (Italy)

    2014-11-15

    We investigated the prognostic value of total metabolic tumour volume (TMTV) in diffuse large B-cell lymphoma (DLBCL). TMTV was measured in 114 patients with newly diagnosed DLBCL who underwent {sup 18}F-FDG PET/CT at baseline before immunochemotherapy. TMTV was computed by summing the volumes of all lymphomatous lesions after applying the local SUVmax threshold of 41 % using semiautomatic software. Prognostic value was assessed by Kaplan-Meier estimates of progression-free survival (PFS) and overall survival (OS). Median follow-up was 39 months. Average pretherapy TMTV was 509 ± 568 cm{sup 3}. The 3-year estimates of PFS were 77 % in the low metabolic burden group (TMTV ≤550 cm{sup 3}) and 60 % in the high metabolic burden group (TMTV >550 cm{sup 3}, p = 0.04), and prediction of OS was even better (87 % vs. 60 %, p = 0.0003). Cox regression showed independence of TMTV for OS prediction (p = 0.002) compared with other pretherapy indices of tumour burden, such as tumour bulk and the International Prognostic Index. Pretherapy TMTV is an independent predictor of outcome in patients with DLBCL. (orig.)

  17. Multi-response data treatment of dynamic and steady state permeation measurement on composite membrane

    Czech Academy of Sciences Publication Activity Database

    Fíla, V.; Bernauer, B.; Hrabánek, Pavel

    2006-01-01

    Roč. 200, 1-3 (2006), s. 120-121 ISSN 0011-9164 R&D Projects: GA AV ČR(CZ) 1QS401250509 Institutional research plan: CEZ:AV0Z40400503 Keywords : composite membrane * physical chemistry * Wicke-Kalenbach permeation Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 0.917, year: 2006

  18. Nutrient Uptake and Metabolism Along a Large Scale Tropical Physical-Chemical Gradient

    Science.gov (United States)

    Tromboni, F.; Neres-Lima, V.; Saltarelli, W. A.; Miwa, A. C. P.; Cunha, D. G. F.

    2016-12-01

    Nutrient spiraling is a whole-system approach for estimating nutrient uptake that can be used to assess aquatic ecosystems' responses to environmental change and anthropogenic impacts. Historically research on nutrient dynamic uptake in streams has focused on single nutrient dynamics and only rarely the stoichiometric uptake has been considered and linked to carbon metabolism driven by autotrophic and heterotrophic production. We investigated the relationship between uptake of phosphate (PO43-), nitrate (NO3-) ammonium (NH4+) and total dissolve nitrogen (DIN)/ PO43-; and gross primary production (GPP), respiration (R), and net ecosystem productivity (NEP) in six relatively pristine streams with differences regarding canopy cover and physical characteristics, located in a large scale gradient from tropical Atlantic Forest to an Atlantic forest/Cerrado (Brazilian Savanna) transition. We carried out whole stream instantaneous additions of PO43-, NO3- and NH4+ added to each stream in combination, using the TASCC (Tracer Additions for Spiraling Curve Characterization) method. Metabolism measurements were performed in the same streams right after uptake was measured, using one-station open channel method and re-aeration estimations for those sites. We found different background concentrations in the streams located in the Atlantic forest compared with the transition area with Cerrado. In general PO43- and NO3- uptake increased with the decreasing of canopy cover, while a positive relation with background concentration better explained NH4+uptake. DIN/PO43- uptake increased with increasing R and NEP. Little work on functional characteristics of pristine streams has been conducted in this region and this work provides an initial characterization on nitrogen and phosphorus dynamics as well as their stoichiometric uptake in streams.

  19. Effect of hygroscopic materials on water vapor permeation and dehumidification performance of poly(vinyl alcohol) membranes

    KAUST Repository

    Bui, T. D.; Wong, Y.; Thu, K.; Oh, S. J.; Kum Ja, M.; Ng, Kim Choon; Raisul, I.; Chua, K. J.

    2017-01-01

    increased with both added hygroscopic material and absorbed water. Water permeation energy varied from positive to negative with higher hygroscopic content. This observation is attributed to a lower diffusion energy and a relatively constant sorption energy

  20. Experimental Evaluation of Hybrid Distillation-Vapor Permeation Process for Efficient Ethanol Recovery from Ethanol-Water Mixtures

    Science.gov (United States)

    The energy demand of distillation-based systems for ethanol recovery and dehydration can be significant, particularly for dilute solutions [1]. An alternative separation process integrating vapor stripping with a vapor compression step and a vapor permeation membrane separation ...