Consumer peach preferences and purchasing behavior: a mixed methods study.

Science.gov (United States)

Kelley, Kathleen M; Primrose, Rachel; Crassweller, Robert; Hayes, John E; Marini, Richard

2016-05-01

Peaches (Prunus persica (L.) Stokes) are grown in several regions throughout the USA, are eaten fresh, and used as ingredients in value-added processed products. An Internet survey was conducted to investigate Mid-Atlantic consumers' fresh and processed peach purchasing behaviors, and whether packaging certain numbers of peaches together, providing information about nutritional content, and other factors would increase purchases. Additionally, laboratory-based sensory testing was used to better understand peel color, texture, sweetness, sourness, and flavor preferences for cultivars commonly grown in the Mid-Atlantic region. Irrespective of fresh peach consumption frequency, certain value-added products were of interest. For some products, interest in purchasing was higher than reported purchasing behavior. Preference for certain fresh peach characteristics, such as peel color, differed between less frequent fresh peach consumers and those who consumed fresh peaches more often. Of the four peach cultivars included in the sensory test, most were liked; however, there were some cultivar differences pertaining to color, texture, sweetness, tartness, and flavor liking. Potential marketing strategies can be developed based on frequency of fresh peach consumption and household demographics. Data can be used to select peaches that best appeal to consumers. © 2015 Society of Chemical Industry. © 2015 Society of Chemical Industry.

Atmospheric fluoride levels in some Ontario peach orchards

Energy Technology Data Exchange (ETDEWEB)

Drowley, W B; Rayner, A C; Jephcott, C M

1963-10-01

As a result of suture injury to peaches, a survey was taken to determine monthly atmospheric fluoride levels in the soft fruit-growing area of the Niagara Peninsula of Ontario. Although the injury was confined to a few orchards, it was found necessary to locate stations for fluoride sampling over a wide area. The limed-paper candle method was used for fluoride collection. Unwashed and washed peach leaves were analyzed for fluoride content. Fluoride was separated from all samples by the Willard-Winter distillation procedure and estimated colorimetrically. It was found that a general background level of atmospheric fluoride existed in the whole surveyed area and that the levels were highest in and near the area of peach injury. The fluoride content of peach leaves was also highest in this area. The coincidence of high fluoride levels and suture injury to peaches suggests that atmospheric fluoride was the causal agent. When lime sprays were applied to peach trees in the damaged area, a reduction in the incidence of suture injury was observed.

Sensory profile of eleven peach cultivars

Directory of Open Access Journals (Sweden)

Francine Lorena Cuquel

2012-03-01

Full Text Available The goal of this study was to evaluate the sensory profile of eleven peach cultivars grown in an experimental orchard located in the city of Lapa (PR, Brazil in two seasons. The peach cultivars analyzed were Aurora I, Chimarrita, Chiripá, Coral, Eldorado, Granada, Leonense, Maciel, Marli, Premier, and Vanguarda. The sensory analysis was performed by previously trained panelists; 20 of them in the first season and 10 in the second season. The sensory evaluation was performed using Quantitative Descriptive Analysis, in which the following attributes were measured: appearance, aroma, flesh color, flesh firmness, flavor, and juiciness. The results showed preference for sweet, soft, and juicy fruits. Chimarrita, Chiripá, and Coral fruits showed better sensorial performance than the other peach cultivars. It was also verified that the analysis of the attributes aroma, flesh firmness, and flavor is enough for performing the sensory profile of peach fruits for in natura consumption.

PeachVar-DB: A Curated Collection of Genetic Variations for the Interactive Analysis of Peach Genome Data.

Science.gov (United States)

Cirilli, Marco; Flati, Tiziano; Gioiosa, Silvia; Tagliaferri, Ilario; Ciacciulli, Angelo; Gao, Zhongshan; Gattolin, Stefano; Geuna, Filippo; Maggi, Francesco; Bottoni, Paolo; Rossini, Laura; Bassi, Daniele; Castrignanò, Tiziana; Chillemi, Giovanni

2018-01-01

Applying next-generation sequencing (NGS) technologies to species of agricultural interest has the potential to accelerate the understanding and exploration of genetic resources. The storage, availability and maintenance of huge quantities of NGS-generated data remains a major challenge. The PeachVar-DB portal, available at http://hpc-bioinformatics.cineca.it/peach, is an open-source catalog of genetic variants present in peach (Prunus persica L. Batsch) and wild-related species of Prunus genera, annotated from 146 samples publicly released on the Sequence Read Archive (SRA). We designed a user-friendly web-based interface of the database, providing search tools to retrieve single nucleotide polymorphism (SNP) and InDel variants, along with useful statistics and information. PeachVar-DB results are linked to the Genome Database for Rosaceae (GDR) and the Phytozome database to allow easy access to other external useful plant-oriented resources. In order to extend the genetic diversity covered by the PeachVar-DB further, and to allow increasingly powerful comparative analysis, we will progressively integrate newly released data. © The Author 2017. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

Commercial scale irradiation for insect disinfestation preserves peach quality

International Nuclear Information System (INIS)

McDonald, Heather; McCulloch, Mary; Caporaso, Fred; Winborne, Ian; Oubichon, Michon; Rakovski, Cyril; Prakash, Anuradha

2012-01-01

Irradiation is approved as a generic quarantine treatment by the US Department of Agriculture, Animal and Plant Health Inspection Service. Due to the effectiveness of irradiation in controlling insects on commodities, there is a growing need to understand the effects of low dose irradiation on fruit quality. The goal of this study was to determine the sensitivity of peaches (Prunus persica) to irradiation, and secondly, to determine the effect of commercial scale treatment on shelf-life, overall quality and consumer liking. Six varieties of peaches were irradiated in small batches at 0.29, 0.49, 0.69 and 0.90 kGy to observe the sensitivity of peaches at different dose levels. Changes in quality were evaluated by 8 trained panelists using descriptive analysis. Sensory characteristics (color, smoothness, aroma, touch firmness, mouth firmness, graininess, overall flavor and off-flavor) were evaluated at 2–4 day intervals and untreated samples served as control. To simulate commercial treatment, peaches were irradiated in pallet quantities at a target dose level of 0.4 kGy. The average absorbed dose was 0.66 kGy with an average dose uniformity ratio of 1.57. Commercially treated peaches were evaluated by 40–80 untrained consumers for acceptability routinely throughout the shelf life. Titratable acidity, Brix, texture and weight loss were also monitored for both commercial and small scale irradiated peaches. There was no dose effect on TA, Brix and weight loss due to irradiation. Peaches irradiated at 0.69 and 0.90 kGy were darker in flesh color, more juicy and less firm as determined by the trained panel and analytical pressure tests. Commercial scale irradiation did not adversely affect shelf life but was seen to enhance ripening. This, however, was perceived as a positive change by consumers. Overall, consumers rated the acceptability of irradiated peaches higher than untreated peaches. Statistical analysis was performed using linear mixed models to find

TWIG BLIGHT AND DEFOLIATION CAUSED BY Colletotrichum horii IN PERSIMMONS IN BRAZIL

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LOUISE LARISSA MAY DE MIO

2015-03-01

Full Text Available Persimmon anthracnose has been a great concern to Brazilian producers. This study aimed to identify and characterized the causal species from Brazilian persimmons byassessing morphological and molecular characteristics and pathogenicity tests. Five fungal isolatesobtained from diseased twigs and fruits were identified as Colletotrichum horii, based on morphologicalcharacteristics and nucleotide sequences of ITS region. Inoculation tests revealed that the fungal isolates caused necrotic spots followed by defoliation of leaves, blight of twigs and buds of potted persimmon plants.

Analysis of Functional Constituents in Mulberry (Morus alba L.) Twigs by Different Cultivars, Producing Areas, and Heat Processings

Science.gov (United States)

Choi, Sang Won; Jang, Yeon Jeong; Lee, Yu Jin; Leem, Hyun Hee; Kim, Eun Ok

2013-01-01

Four functional constituents, oxyresveratrol 3′-O-β-D-glucoside (ORTG), oxyresveratrol (ORT), t-resveratrol (RT), and moracin (MC) were isolated from the ethanolic extract of mulberry (Morus alba L.) twigs by a series of isolation procedures, including solvent fractionation, and silica-gel, ODS-A, and Sephadex LH-20 column chromatographies. Their chemical structures were identified by NMR and FABMS spectral analysis. Quantitative changes of four phytochemicals in mulberry twigs were determined by HPLC according to cultivar, producing area, and heat processing. ORTG was a major abundant compound in the mulberry twigs, and its levels ranged from 23.7 to 105.5 mg% in six different mulberry cultivars. Three other compounds were present in trace amounts (<1 mg/100 g) or were not detected. Among mulberry cultivars examined, “Yongcheon” showed the highest level of ORTG, whereas “Somok” had the least ORTG content. Levels of four phytochemicals in the mulberry twigs harvested in early September were higher than those harvested in early July. Levels of ORTG and ORT in the “Cheongil” mulberry twigs produced in the Uljin area were higher than those produced in other areas. Generally, levels of ORTG and ORT in mulberry twigs decreased with heat processing, such as steaming, and microwaving except roasting, whereas those of RT and MC did not considerably vary according to heat processing. These results suggest that the roasted mulberry twigs may be useful as potential sources of functional ingredients and foods. PMID:24551827

Goldspotted oak borer: Field identification guide

Science.gov (United States)

S. Hishinuma; T.W. Coleman; M.L. Flint; S.J. Seybold

2011-01-01

The goldspotted oak borer (GSOB), Agrilus auroguttatus (Coleoptera: Buprestidae), is a flatheaded borer new to California that poses a significant threat to oak trees. The pest is native to southeastern Arizona, although a related species occurs in southern Mexico and northern Guatemala. GSOB was first collected and identified in California in 2004 in San Diego County...

Chemical compositions and antimicrobial activity of twig essential ...

African Journals Online (AJOL)

Aghomotsegin

2016-03-09

Mar 9, 2016 ... The chemical composition of twig essential oils of Xylopia malayana, Xylopia elliptica and Xylopia fusca ... Volatile constituents and bioactivity studies are available in the literature on Xylopia aethiopica (Issakou et al., 2014;. Sylvain et al, 2014; Vyry et al, 2014), Xylopia longifolia. (Fourier et al, 1993), ...

Within-twig leaf distribution patterns differ among plant life-forms in a subtropical Chinese forest.

Science.gov (United States)

Meng, Fengqun; Cao, Rui; Yang, Dongmei; Niklas, Karl J; Sun, Shucun

2013-07-01

In theory, plants can alter the distribution of leaves along the lengths of their twigs (i.e., within-twig leaf distribution patterns) to optimize light interception in the context of the architectures of their leaves, branches and canopies. We hypothesized that (i) among canopy tree species sharing similar light environments, deciduous trees will have more evenly spaced within-twig leaf distribution patterns compared with evergreen trees (because deciduous species tend to higher metabolic demands than evergreen species and hence require more light), and that (ii) shade-adapted evergreen species will have more evenly spaced patterns compared with sun-adapted evergreen ones (because shade-adapted species are generally light-limited). We tested these hypotheses by measuring morphological traits (i.e., internode length, leaf area, lamina mass per area, LMA; and leaf and twig inclination angles to the horizontal) and physiological traits (i.e., light-saturated net photosynthetic rates, Amax; light saturation points, LSP; and light compensation points, LCP), and calculated the 'evenness' of within-twig leaf distribution patterns as the coefficient of variation (CV; the higher the CV, the less evenly spaced leaves) of within-twig internode length for 9 deciduous canopy tree species, 15 evergreen canopy tree species, 8 shade-adapted evergreen shrub species and 12 sun-adapted evergreen shrub species in a subtropical broad-leaved rainforest in eastern China. Coefficient of variation was positively correlated with large LMA and large leaf and twig inclination angles, which collectively specify a typical trait combination adaptive to low light interception, as indicated by both ordinary regression and phylogenetic generalized least squares analyses. These relationships were also valid within the evergreen tree species group (which had the largest sample size). Consistent with our hypothesis, in the canopy layer, deciduous species (which were characterized by high LCP, LSP and

Apple and peach consumption habits across European countries.

Science.gov (United States)

Konopacka, D; Jesionkowska, K; Kruczyńska, D; Stehr, R; Schoorl, F; Buehler, A; Egger, S; Codarin, S; Hilaire, C; Höller, I; Guerra, W; Liverani, A; Donati, F; Sansavini, S; Martinelli, A; Petiot, C; Carbó, J; Echeverria, G; Iglesias, I; Bonany, J

2010-12-01

The aim of this study was to gain information concerning apple and peach consumption frequency within different European countries in relation to age and gender. The survey was a part of a complex experiment with the aim of evaluating consumers' preferences towards new varieties, and the data is based on the self-reported declarations of respondents, male and female, between 15 and 70 years old. 4271 consumers from 7 European countries were invited to supply information about their apple consumption habits, whereas 499 respondents from 5 countries answered questions relating to frequency of peach and nectarine consumption. In both, the apple and the peach surveys, data analysis of declared intake showed significant differences between nationalities. The highest apple consumption was in Poland, where over 55% declared a consumption of more than 5 apples per week. In comparison, Italian consumers most often indicated eating 3-5 apples per week (39.3%). The lowest apple consumption was in the Netherlands and Spain. In the case of peaches, the highest consumption was indicated in France where 48% of respondents declared a peach consumption of 3-5 per week with 40% eating more than 5 fruits per week. The lowest peach intake was declared in Germany. Irrespective of country women were shown to eat more apples that men. Furthermore, the group of older people (61-70 years) consume apples more often than the adult group (36-60), while within the youngest group of consumers (16-35) eating apples was not at all popular. As with apples females declared a higher peach consumption, and again significantly lower fruit consumption by the youngest group (16-35) was indicated. Although the availability of fruit at the market remains a prime factor in determining apple and peach consumption, our survey confirmed the trends of declining this popular fruit intake by the younger generation, as well as the persistent tendency of lower frequency of fruit consumption among men than women

Physicochemical, antioxidant, and sensorial properties of peach ...

African Journals Online (AJOL)

Preparation of varieties of processed peach products could be a good option to supply in lean season. Objective of the study was to assess the quality characteristics of peach snacks prepared from 11 different cultivars. We investigated the physicochemical (soluble solid, titratable acidity, hardness and dry yield), antioxidant ...

Metode de atracţie şi localizare a faunei utile în agrocenoza culturii de piersic ca factor biologic de control al densităţii speciilor dăunătoare

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Mihai BATCO

2016-06-01

Full Text Available The use of such biorational means as nectariferous plants (Eruca sativa, Fagopyrum esculentum, Phacelia tanacetifolia, Satureja hortensis, Lobulalaria maritima, Dracocephalum moldavica and lawn grasses, protein-carbohydrate compositions and methyl salicylate as factors influencing the beneficial fauna in peach orchard agrocenosis has contributed to the attraction and location of 26 species of Encyrtidae belonging to 19 genera. The species capable to influence the numeric dynamics of economically important species such as oriental moth (Grapholita molesta Busck, peach twig borer (Anarsia lineatella Z, San Jose scale (Diaspidiotus perniciosus Comst., fruit apple scale (Eulecanium coryli L. were nominated. Encyrtidae species whose hosts are potential pests of peach trees, as well as hyperparasite species and parasites of beneficial predatory insects were registered. The stages for timely application of biorational means for useful fauna in the peach orchard agrocenosis during the vegetation period were established. Rezumat. Aplicarea mijloacelor bioraţionale precum culturile nectarifere (Eruca sativa, Fagopyrum esculentum, Phacelia tanacetifolia, Satureja hortensis, Lobulalaria maritima, Dracocephalum moldavica şi iarba de gazon, compoziţiile proteico-glucidice şi metilsalicilatul ca factori de influenţă asupra faunei benefice în agrocenoza culturii de piersic au contribuit la atracţia şi localizarea a 26 specii de Encyrtidae, care se atribuie la 19 genuri. Sunt nominalizate speciile capabile de a influenţa dinamica numerică a speciilor economic importante, aşa ca molia orientală (Grapholita molesta Busck, molia vărgată (Anarsia lineatella Z, păduchele din San Jose (Diaspidiotus perniciosus Comst., păduchele ţestos fals al mărului (Eulecanium coryli L.. Au fost semnalate specii de Encyrtidae ale căror gazde sunt dăunători potenţiali ai piersicului şi de asemenea specii hiperparazite şi paraziţi ai insectelor pr

Oxyresveratrol, a Stilbene Compound from Morus alba L. Twig Extract Active Against Trichophyton rubrum.

Science.gov (United States)

Lu, Hai-Peng; Jia, Ya-Nan; Peng, Ya-Lin; Yu, Yan; Sun, Si-Long; Yue, Meng-Ting; Pan, Min-Hui; Zeng, Ling-Shu; Xu, Li

2017-12-01

Morus alba L. (mulberry) twig is known to have an inhibitory effect on pathogens in traditional Chinese medicine. In the present study, the dermophytic fungus, Trichophyton rubrum, was used to evaluate the inhibitory effect of total M. alba twig extract and extracts obtained using solvents with different polarities by the method of 96-well MTT colorimetry. The main active substance was isolated and identified by tracking its activity. In addition, the inhibitory effects of active extracts and a single active substance were investigated in combination with miconazole nitrate. Our data indicated that ethyl acetate extracts of mulberry twig (TEE) exhibited a desired inhibitory activity on T. rubrum with the minimum inhibitory concentration (MIC) of 1.000 mg/mL. With activity tracking, the main substance showing antimicrobial activity was oxyresveratrol (OXY), which was isolated from TEE. Its MIC for inhibiting the growth of T. rubrum was 0.500 mg/mL. The combined use of miconazole nitrate and OXY showed a synergistic inhibitory effect, as shown by a significant decrease in the MIC of both components. Based on the OXY content in TEE, the contribution rate of OXY to the inhibitory effect of TEE on T. rubrum was 80.52%, so it was determined to be the main antimicrobial substance in M. alba twig. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.

The braconid parasitoids (Hymenoptera: Braconidae) of Kermania pistaciella Amsel (Lepidoptera: Tineidae: Hieroxestinae) in Iran

NARCIS (Netherlands)

Achterberg, van C.; Mehrnejad, M.R.

2002-01-01

Two species of Braconidae (Chelonus kermakiae (Tobias, 2001) (Cheloninae) and Centistidea pistaciella spec. nov. (Miracinae)) have been reared from the pistachio twig borer moth (Kermania pistaciella Amsel) (Tineidae). Both species are described and illustrated; Centistidea pistaciella spec. nov. is

Fungicide efficacy in peach rusty spot control in Serbia

OpenAIRE

Dolovac Nenad; Miletić Novica; Aleksić Goran; Savić Dušan; Živković Svetlana; Trkulja Nenad; Bulajić Aleksandra

2010-01-01

Rusty Spot has long been known as a harmful peach disease in many parts of the world. During the past several years, rusty spot infection of the late-maturing peach cultivars (Summerset, Suncrest, Fayette and O’Henry) caused significant yield losses in Serbia. Although the etiology of the disease is still unknown, there are numerous studies attempting to set a strategy for its control and recommend appropriate chemical and other peach protection methods. Ho...

(Zea mays L.) GENOTYPES BY LEPIDOPTEROUS STEM BORERS

African Journals Online (AJOL)

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The three maize endosperm types used in the experiment were susceptible to stem borer infestation, but there was no statistical difference with respect to stem borer infestation and severity of damage for July and August cropping, although, sweet corn tended to be more susceptible than the other endosperm types (flint and.

Morphological and genetic perspectives of peach fruit responses to spring frost

Science.gov (United States)

Spring frost is one of the most unpredictable cropping factors in many peach production areas. A severe spring frost can wipe out an entire peach crop whereas a mild spring frost may naturally help thinning as is common practice in commercial peach production. The extent of frost damage depends on t...

Inoculation methods and aggressiveness of five Fusarium species against peach palm

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Tiago Miguel Jarek

2018-04-01

Full Text Available ABSTRACT: Fusarium wilt is a major disease which affects peach palm (Bactris gasipaes Kunth.var gasipaes Henderson. This study aimed to evaluate inoculation methods and aggressiveness of isolates of five Fusarium species on peach palm. Fusarium proliferatum can infect the leaves, stem, and roots of peach palm. F. proliferatum, F. oxysporum species complex (FOSC, F. verticillioides, F. solani species complex (FSSC, and Gibberella fujikuroi species complex (GFSC are pathogenic to peach palm. The use of Fusarium-colonized ground corn for root inoculation was effective and reduced the level of damage to plants.

Regulation of anthocyanin biosynthesis in peach fruits.

Science.gov (United States)

Rahim, Md Abdur; Busatto, Nicola; Trainotti, Livio

2014-11-01

MYB10.1 and MYB10.3, with bHLH3, are the likely regulators of anthocyanin biosynthesis in peach fruit. MYB10.1/2/3 forms a cluster on the same genomic fragment where the Anther color ( Ag ) trait is located. Anthocyanins are bioactive compounds responsible for the pigmentation of many plant parts such as leaves, flowers, fruits and roots, and have potential benefits to human health. In peach [Prunus persica (L.) Batsch], peel color is a key determinant for fruit quality and is regulated by flavonoids including anthocyanins. The R2R3 MYB transcription factors (TFs) control the expression of anthocyanin biosynthetic genes with the help of co-activators belonging to the basic-helix-loop-helix (bHLH) and WD40 repeat families. In the peach genome six MYB10-like and three bHLH-like TFs were identified as candidates to be the regulators of the anthocyanin accumulation, which, in yellow flesh fruits, is highest in the peel, abundant in the part of the mesocarp surrounding the stone and lowest in the mesocarp. The expression of MYB10.1 and MYB10.3 correlates with anthocyanin levels of different peach parts. They also have positive correlation with the expression of key structural genes of the anthocyanin pathway, such as CHS, F3H, and UFGT. Functions of peach MYB10s were tested in tobacco and shown to activate key genes in the anthocyanin pathway when bHLHs were co-expressed as partners. Overexpression of MYB10.1/bHLH3 and MYB10.3/bHLH3 activated anthocyanin production by up-regulating NtCHS, NtDFR and NtUFGT while other combinations were not, or much less, effective. As three MYB10 genes are localized in a genomic region where the Ag trait, responsible for anther pigmentation, is localized, it is proposed they are key determinant to introduce new peach cultivars with higher antioxidant level and pigmented fruit.

HOW to Identify and Control Sugar Maple Borer

Science.gov (United States)

William H. Hoffard; Philip T. Marshall

1978-01-01

The sugar maple borer, Glycobius speciosus (Say), a long-horned wood boring beetle, is a common pest of sugar maple (the only known host) throughout the range of the tree. Although borer-caused mortality is rare, infestations lead to value loss through lumber defect caused by larval galleries, discoloration, decay, and twisted grain.

Indole alkaloids from leaves and twigs of Rauvolfia verticillata.

Science.gov (United States)

Zhang, Bing-Jie; Peng, Lei; Wu, Zhi-Kun; Bao, Mei-Fen; Liu, Ya-Ping; Cheng, Gui-Guang; Luo, Xiao-Dong; Cai, Xiang-Hai

2013-01-01

Seven new indole alkaloids, rauverines A-G (1-7), and 19 known indole alkaloids were isolated from the leaves and twigs of Rauvolfia verticillata. All compounds showed no cytotoxicity against five human cancer cell lines, human myeloid leukemia (HL-60), hepatocellular carcinoma (SMMC-7721), lung cancer (A-549), breast cancer (MCF-7), and colon cancer (SW480) cells.

A Dendrochronological Analysis of Red Oak Borer Abundance

Science.gov (United States)

Rose-Marie Muzika; Richard P. Guyette

2004-01-01

Unprecedented outbreaks of red oak borer (Enaphalodes rufulus Haldemann) have occurred in the lower Midwestern United States. Although generally not a mortality agent, red oak borer appears to contribute to general oak decline and mortality. The objective of this project was to explore dendrochronology as a means of determining the role of tree age,...

Stem Photosynthesis of Twig and Its Contribution to New Organ Development in Cutting Seedlings of Salix Matsudana Koidz.

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Junxiang Liu

2018-04-01

Full Text Available The objective of this study was to illustrate the photosynthetic characteristics of current twigs of Salix matsudana Koidz., and clarify the effect of stem photosynthesis on the new organ development in cutting seedlings. Excised twigs were taken as the experimental samples. The response of the stem photosynthesis rate to increasing light intensity and the effective photochemical efficiency of the cross section of the twig were determined. Then, twigs were used as cuttings and exposed to 0, 20, and 100 μmol m−2 s−1 light intensities, respectively, to achieve distinctive stem photosynthetic rates. After 14 days of treatment, stem water and non-structural carbohydrate (NSC content, as well as the biomass and carbon isotopic composition, of new organs in the cutting seedlings under different light treatments were examined. The results showed that the gross photosynthetic rate significantly increased within 400 μmol m−2 s−1 of light intensity, and the maximum rate was approximately 1.27 μmol m−2 s−1. The effective photochemical efficiency of the PSⅡ of the cortex was significantly higher than the inner tissues in the cross section of the twig. When twig cuttings were exposed to different light intensities, stem water and starch content, as well as bud and root biomass, were significantly higher in the cutting seedling subjected to 100 μmol m−2 s−1 than the case treated in darkness; however, the bud δ13C trend was the opposite. Stem photosynthesis played a positive role in the maintenance of stem water and starch supply for the cutting seedlings, and 13C depleted assimilates produced by stem photosynthesis contributed to bud biomass, revealing that stem photosynthesis promotes organ development in cutting seedlings of Salix matsudana.

Population dynamics and distribution of the coffee berry borer ...

African Journals Online (AJOL)

Population dynamics and distribution of coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Scolytidae) were studied on Coffea arabica L. in southwestern region of Ethiopia. Thirty coffee trees were sampled at weekly intervals from 2000 to 2001. Findings of this study showed that coffee berry borer population ...

Feeding behavior of lambs fed diets containing peach palm meal

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Alana Batista dos Santos

2016-06-01

Full Text Available The aim of this study was to evaluate the feeding behavior of lambs fed diets containing peach palm meal replacing maize (0, 10, 40, 60, and 85% DM. Thirty Santa Inês sheep with an average initial body weight of 21.6 ± 0.87 kg were distributed in a completely randomized design with five diets and six replicates. Feeding time in min kg–1 DM and min kg–1 NDFap increased by 34 min and 99.6 min, respectively, with each level of substitution of maize for the peach palm meal. Rumination and chewing times, in min kg–1 DM and min kg–1 NDF, also increased in response to the substitution of maize for peach palm meal. When expressed in min day–1, rumination and chewing activities decreased by 12.4 and 14.6 min, respectively, as the amount of peach palm meal in the concentrate was increased. The time spent idle increased linearly (P < 0.05, by 14.6 min day–1, with the replacement levels, compared with the control diet. Peach palm meal in the composition of sheep diets reduces the intakes of dry matter and fiber and decreases the feed and rumination efficiencies. Replacing maize by peach palm meal increases the feeding time and rumination and chewing activities of feedlot lambs.

Protecting black ash from the emerald ash borer

Science.gov (United States)

Les Benedict

2010-01-01

Black ash (Fraxinus nigra) is an important resource for Tribes in the Northeast and Great Lakes regions of the North American continent. Ash in North America is being threatened with widespread destruction as a result of the introduction of emerald ash borer beetle (Agrilus planipennis) in 2002. Measures are being taken to slow the spread of emerald ash borer beetle....

Chemical composition of essential oils from needles and twigs of balkan pine (Pinus peuce grisebach) grown in Northern Greece.

Science.gov (United States)

Koukos, P K; Papadopoulou, K I; Patiaka, D T; Papagiannopoulos, A D

2000-04-01

The composition of essential oils from twigs and needles of Balkan pine (Pinus peuce Gris.) grown in northern Greece was investigated. The compounds were identified by using GC-MS analysis. The twig oil was rich in alpha-pinene (7.38%), beta-pinene (12.46%), beta-phellandrene (26.93%), beta-caryophyllene (4.48%), and citronellol (12.48%), and the needle oil was rich in alpha-pinene (23.07%), camphene (5.52%), beta-pinene (22.00%), beta-phellandrene (6.78%), bornyl acetate (9.76%), beta-caryophyllene (3.05%), and citronellol (13.42%). The mean oil yield was 2.85% for twigs and 0. 57% for needles.

Individual sugar and acid composition within southeastern peach germplasm

Science.gov (United States)

eaches grown in the southeast are valued for their acidic, sweet flavor. A complex mixture of various sugars and acids at different ratios play a key role in determining these unique peach flavor attributes. To understand the flavor profile of fresh market peaches, individual sugar and acid compone...

New phenolic compounds from the twigs of Artocarpus heterophyllus.

Science.gov (United States)

Di, X; Wang, S; Wang, B; Liu, Y; Yuan, H; Lou, H; Wang, X

2013-02-01

Two new chalcones, artocarpusins A and B (1 and 2), one new flavone, artocarpusin C (3), one new 2-arylbenzofuran derivative, artocarstilene A (4), and 15 flavonoids were isolated from the twigs of Artocarpus heterophyllus. Their structures were established on the basis of extensive spectroscopic analysis. Compounds 9 and 16 showed moderate inhibitory activity on the proliferation of the PC-3 and H460 cell lines.

Overwintering physiology of the rice stem borer larvae, Chilo ...

African Journals Online (AJOL)

The rice stem borer, Chilo suppressalis (Walker), is a major rice pest around the world. A strong ability of the rice stem borer to adapt/resist cold temperature (cold hardiness) contributes to its survival through winter. However, the physiological mechanism of its cold hardiness is poorly understood. In this study, we ...

Evaluation of stem borer resistance management strategies for Bt ...

African Journals Online (AJOL)

Stem borers are the major insect pests of maize in Kenya. The use of Bacillus thuringiensis (Bt) technology is an effective way of controlling lepidopteran pests. However, the likelihood of development of resistance to the Bt toxins by the target stem borer species is a concern. Forages, sorghum and maize varieties were ...

White Fringetree as a Novel Larval Host for Emerald Ash Borer.

Science.gov (United States)

Cipollini, Don

2015-02-01

Emerald ash borer is an invasive Asian pest of ash species in North America. All North American species of ash tested so far are susceptible to it, but there are no published reports of this insect developing fully in non-ash hosts in the field in North America. I report here evidence that emerald ash borer can attack and complete development in white fringetree, Chionanthus virginicus L., a species native to the southeastern United States that is also planted ornamentally. Four of 20 mature ornamental white fringetrees examined in the Dayton, Ohio area showed external symptoms of emerald ash borer attack, including the presence of adult exit holes, canopy dieback, and bark splitting and other deformities. Removal of bark from one of these trees yielded evidence of at least three generations of usage by emerald ash borer larvae, several actively feeding live larvae, and a dead adult confirmed as emerald ash borer. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Fungicide Efficacy in Peach Rusty Spot Control in Serbia

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Nenad Dolovac

2010-01-01

Full Text Available Rusty Spot has long been known as a harmful peach disease in many parts of the world. During the past several years, rusty spot infection of the late-maturing peach cultivars (Summerset, Suncrest, Fayette and O’Henry caused significant yield losses in Serbia.Although the etiology of the disease is still unknown, there are numerous studies attempting to set a strategy for its control and recommend appropriate chemical and other peach protection methods. However, because of specific environmental conditions in Serbia, recommended protection method using repeated fungicide treatments, starting from petal fall, did not prove to be efficient and the rate of infection in some susceptible peach cultivars reached 100%. In 2003 and 2004 a field trial was conducted in order to test the efficacy of fungicides (a.i. kresoxim-methyl, flusilazole and sulfur for the efficient control of Rusty Spot epidemics. The trial was carried out under conditions of natural infection on the peach cv. Summerset at the locality of Bela Crkva, Serbia. In the ntreated control plots,high disease incidence was recorded with the percentage of affected fruit surface ranging from 33.5% in the first, up to the 35.4% in the second year of the trail. Among fungicides included in the trial, kresoxim-methyl proved to be the most efficient (90.25% in the first and 91.12% in the second year of the trial, flusilazole exhibited lower efficacy (87.28% and 80.61%, respectively while sulfur was the least efficient 82.33% and 80.30%, respectively. Determination of the most efficient fungicide for the peach rusty spot control in Serbia provides basic nformation for further investigations which will include optimization of treatment terms, as well as additional agro-technical control measures.

Genetic dissection of Sharka disease tolerance in peach (P. persica L. Batsch).

Science.gov (United States)

Cirilli, Marco; Rossini, Laura; Geuna, Filippo; Palmisano, Francesco; Minafra, Angelantonio; Castrignanò, Tiziana; Gattolin, Stefano; Ciacciulli, Angelo; Babini, Anna Rosa; Liverani, Alessandro; Bassi, Daniele

2017-11-03

Plum pox virus (PPV), agent of Sharka disease, is the most important quarantine pathogen of peach (P. persica L. Batsch). Extensive evaluation of peach germplasm has highlighted the lack of resistant sources, while suggesting the presence of a quantitative disease resistance, expressed as reduction in the intensity of symptoms. Unravelling the genetic architecture of peach response to PPV infection is essential for pyramiding resistant genes and for developing more tolerant varieties. For this purpose, a genome-wide association (GWA) approach was applied in a panel of accessions phenotyped for virus susceptibility and genotyped with the IPSC peach 9 K SNP Array, and coupled with an high-coverage resequencing of the tolerant accession 'Kamarat'. Genome-wide association identified three highly significant associated loci on chromosome 2 and 3, accounting for most of the reduction in PPV-M susceptibility within the analysed peach population. The exploration of associated intervals through whole-genome comparison of the tolerant accession 'Kamarat' and other susceptible accessions, including the PPV-resistant wild-related species P. davidiana, allow the identification of allelic variants in promising candidate genes, including an RTM2-like gene already characterized in A. thaliana. The present study is the first effort to identify genetic factors involved in Sharka disease in peach germplasm through a GWA approach. We provide evidence of the presence of quantitative resistant loci in a collection of peach accessions, identifying major loci and highly informative SNPs that could be useful for marker assisted selection. These results could serve as reference bases for future research aimed at the comprehension of genetic mechanism regulating the complex peach-PPV interaction.

Control of Busseola fusca and Chilo partellus stem borers by ...

African Journals Online (AJOL)

Previous testing of several public Bacillus thuringiensis (Bt)-maize events did not show control of the African stem borer (Busseola fusca Fuller), an important stem borer species, without which stewardship would be compromised by the possibility of rapid development of resistance to Bt deltaendotoxins. This study was ...

A bottom-up model to describe consumers’ preferences towards late season peaches

Energy Technology Data Exchange (ETDEWEB)

Groot, E.; Albisu, L.M.

2015-07-01

Peaches are consumed in Mediterranean countries since ancient times. Nowadays there are few areas in Europe that produce peaches with Protected Designation of Origin (PDO), and the Calanda area is one of them. The aim of this work is to describe consumers’ preferences towards late season PDO Calanda peaches in the city of Zaragoza, Spain, by a bottom-up model. The bottom-up model proves greater amount of information than top-down models. In this approach it is estimated one utility function per consumer. Thus, it is not necessary to make assumptions about preference distributions and correlations across respondents. It was observed that preference distributions were neither normal nor independently distributed. If those preferences were estimated by top-down models, conclusions would be biased. This paper also explores a new way to describe preferences through individual utility functions. Results show that the largest behavioural group gathered origin sensitive consumers. Their utility increased if the peaches were produced in the Calanda area and, especially, when peaches had the PDO Calanda brand. In sequence, the second most valuable attribute for consumers was the price. Peach size and packaging were not so important on purchase choice decision. Nevertheless, it is advisable to avoid trading smallest size peaches (weighting around 160 g/fruit). Traders also have to be careful by using active packaging. It was found that a group of consumers disliked this kind of product, probably, because they perceived it as less natural. (Author)

Characterization of a New Flavone and Tyrosinase Inhibition Constituents from the Twigs of Morus alba L.

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Zhang, Long; Tao, Guanjun; Chen, Jie; Zheng, Zong-Ping

2016-09-02

The twigs of Morus alba L. were found to show strong tyrosinase inhibition activity, and the responsible active components in the extract were further investigated in this study. A flavone, named morusone (1), and sixteen known compounds 2-17 were isolated from M. alba twigs and their structures were identified by interpretation of the corresponding ESI-MS and NMR spectral data. In the tyrosinase inhibitory test, the compounds steppogenin (IC50 0.98 ± 0.01 µM), 2,4,2',4'-tetrahydroxychalcone (IC50 0.07 ± 0.02 µM), morachalcone A (IC50 0.08 ± 0.02 µM), oxyresveratrol (IC50 0.10 ± 0.01 µM), and moracin M (8.00 ± 0.22 µM) exhibited significant tyrosinase inhibition activities, much stronger than that of the positive control kojic acid. These results suggest that M. alba twig extract should served as a good source of natural tyrosinase inhibitors for use in foods as antibrowning agents or in cosmetics as skin-whitening agents.

Characterization of a New Flavone and Tyrosinase Inhibition Constituents from the Twigs of Morus alba L.

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Long Zhang

2016-09-01

Full Text Available The twigs of Morus alba L. were found to show strong tyrosinase inhibition activity, and the responsible active components in the extract were further investigated in this study. A flavone, named morusone (1, and sixteen known compounds 2–17 were isolated from M. alba twigs and their structures were identified by interpretation of the corresponding ESI-MS and NMR spectral data. In the tyrosinase inhibitory test, the compounds steppogenin (IC50 0.98 ± 0.01 µM, 2,4,2′,4′-tetrahydroxychalcone (IC50 0.07 ± 0.02 µM, morachalcone A (IC50 0.08 ± 0.02 µM, oxyresveratrol (IC50 0.10 ± 0.01 µM, and moracin M (8.00 ± 0.22 µM exhibited significant tyrosinase inhibition activities, much stronger than that of the positive control kojic acid. These results suggest that M. alba twig extract should served as a good source of natural tyrosinase inhibitors for use in foods as antibrowning agents or in cosmetics as skin-whitening agents.

The leaf size-twig size spectrum in evergreen broadleaved forest of ...

African Journals Online (AJOL)

Compared to deciduous broad-leaved species, the evergreen broad-leaved species were smaller in total leaf area for a given cross-sectional area or stem mass. This suggests that the species would support less leaf area at a given twig cross-sectional area with increasing environmental stress. And the life form can modify ...

The impact of predators on maize stem borers in coastal Kenya

NARCIS (Netherlands)

Bonhof, M.

2000-01-01

Damage caused by Lepidopteran stem borers is one of the most important constraints to maize production in East and southern Africa. Of the stem borer complex, Chilo partellus Swinhoe is the most abundant species in lowland areas. Although control strategies exist, many

The Synthesis and Characterization of Peach-Like ZnO

International Nuclear Information System (INIS)

Kamalianfar, A.; Halim, S. A.; Navasery, M.; Din Fasih, Ud; Lim, K. P.; Chen, S. K.; Jahromi, Siamak Pilban; Zahedi, J. A. M.

2012-01-01

Peach-like ZnO microstructures are synthesized using vapor phase transport on MgO (001) substrates with a copper oxide (60 nm) buffer layer. The structure and morphology of the product are investigated using an x-ray diffractometer (XRD) and a field-emission scanning electron microscope. The peaches have an average diameter of 3 μm and a wurtzite structure. To study the optical properties, photoluminescence (PL) and Raman spectroscopy are employed. A strong UV emission at 380 nm in the PL spectra is observed, and a sharp and dominant peak at 437 cm −1 in the Raman spectrum can be assigned to the good crystallization of obtained product. In addition, the growth mechanism of the peach-like ZnO structure is tentatively investigated based on the EDX analysis and growth time

Preliminary Studies on the Occurrence of Stem Borers and the ...

African Journals Online (AJOL)

A field experiment was carried out to study the occurrence of stem-borers and the incidence of stalk rot under varying population densities (64,000, 32,000, and 21,333 plants/ha) of two common cultivars (TZSR-Y and DMRZ-Y) of maize. Whereas the percentage of plants with stem borers infestation and those with stem ...

Burdock fructooligosaccharide enhances biocontrol of Rhodotorula mucilaginosa to postharvest decay of peaches.

Science.gov (United States)

Zhang, Hongyin; Liu, Zhouyang; Xu, Baitian; Chen, Keping; Yang, Qiya; Zhang, Qiuyun

2013-10-15

The influence of adding burdock fructooligosaccharide (BFO) in the culture media on the efficacy of Rhodotorula mucilaginosa in controlling postharvest decay of peaches and its possible mode of action were investigated. The antagonistic activity of R. mucilaginosa to Rhizopus decay and blue mold decay of peaches was greatly enhanced through cultivation in the nutrient yeast dextrose agar (NYDA) medium amended with BFO at the concentration of 0.32%, compared with that cultivated in NYDB without BFO. R. mucilaginosa at 1×10(8) cells/mL cultivation in the NYDB media did not reduce the natural decay incidence of peaches, compared with the control after 30 d at 4 °C followed by 7d at 20 °C. However, R. mucilaginosa cultivation in the NYDB media amended with BFO at the concentration of 0.32% reduced the natural decay incidence of peaches. The population of R. mucilaginosa harvested from NYDB amended with BFO at 0.32% increased rapidly in peach wounds compared to that harvested from NYDB without BFO no matter peaches were stored at 20 °C or 4 °C. The activities of chitinase and β-1,3-glucanase of cell-free culture filtrate of R. mucilaginosa harvested from NYDB amended with BFO at 0.32% were higher than that at other concentrations and the control. Copyright © 2013 Elsevier Ltd. All rights reserved.

The leaf size-twig size spectrum in evergreen broad- leaved forest of ...

African Journals Online (AJOL)

STORAGESEVER

2010-06-07

Jun 7, 2010 ... al., 1998; Brouat and McKey, 2001; Preston and Ackerly,. 2003; Westoby and Wright, 2003), few studies have examined the scaling relationship in relation to environ- mental gradients. In order to examine the response of the leaf size-twig size relationship to environmental variations, we investi-.

Carbon Monoxide Fumigation Improved the Quality, Nutrients, and Antioxidant Activities of Postharvest Peach

Science.gov (United States)

Li, Ying; Pei, Fei

2014-01-01

Peaches (Prunus persica cv. Yanhong) were fumigated with carbon monoxide (CO) at 0, 0.5, 5, 10, and 20 μmol/L for 2 hours. The result showed that low concentration CO (0.5–10 μmol/L) might delay the decrease of firmness and titrable acid content, restrain the increase of decay incidence, and postpone the variation of soluble solids content, but treating peaches with high concentration CO (20 μmol/L) demonstrated adverse effects. Further research exhibited that exogenous CO could induce the phenylalnine ammonialyase activity, maintain nutrient contents such as Vitamin C, total flavonoid, and polyphenol, and enhance antioxidant activity according to reducing power and 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl radical scavenging activity. Treating peaches with appropriate concentration CO was beneficial to the quality, nutrients, and antioxidant activity of postharvest peaches during storage time. Therefore, CO fumigation might probably become a novel method to preserve postharvest peach and other fruits in the future. PMID:26904651

Evolutionary Genomics of Peach and Almond Domestication

Directory of Open Access Journals (Sweden)

Dianne Velasco

2016-12-01

Full Text Available The domesticated almond [Prunus dulcis (L. Batsch] and peach [P. persica (Mill. D. A. Webb] originated on opposite sides of Asia and were independently domesticated ∼5000 yr ago. While interfertile, they possess alternate mating systems and differ in a number of morphological and physiological traits. Here, we evaluated patterns of genome-wide diversity in both almond and peach to better understand the impacts of mating system, adaptation, and domestication on the evolution of these taxa. Almond has around seven times the genetic diversity of peach, and high genome-wide FST values support their status as separate species. We estimated a divergence time of ∼8 MYA (million years ago, coinciding with an active period of uplift in the northeast Tibetan Plateau and subsequent Asian climate change. We see no evidence of a bottleneck during domestication of either species, but identify a number of regions showing signatures of selection during domestication and a significant overlap in candidate regions between peach and almond. While we expected gene expression in fruit to overlap with candidate selected regions, instead we find enrichment for loci highly differentiated between the species, consistent with recent fossil evidence suggesting fruit divergence long preceded domestication. Taken together, this study tells us how closely related tree species evolve and are domesticated, the impact of these events on their genomes, and the utility of genomic information for long-lived species. Further exploration of this data will contribute to the genetic knowledge of these species and provide information regarding targets of selection for breeding application, and further the understanding of evolution in these species.

Evolutionary Genomics of Peach and Almond Domestication.

Science.gov (United States)

Velasco, Dianne; Hough, Josh; Aradhya, Mallikarjuna; Ross-Ibarra, Jeffrey

2016-12-07

The domesticated almond [Prunus dulcis (L.) Batsch] and peach [P. persica (Mill.) D. A. Webb] originated on opposite sides of Asia and were independently domesticated ∼5000 yr ago. While interfertile, they possess alternate mating systems and differ in a number of morphological and physiological traits. Here, we evaluated patterns of genome-wide diversity in both almond and peach to better understand the impacts of mating system, adaptation, and domestication on the evolution of these taxa. Almond has around seven times the genetic diversity of peach, and high genome-wide [Formula: see text] values support their status as separate species. We estimated a divergence time of ∼8 MYA (million years ago), coinciding with an active period of uplift in the northeast Tibetan Plateau and subsequent Asian climate change. We see no evidence of a bottleneck during domestication of either species, but identify a number of regions showing signatures of selection during domestication and a significant overlap in candidate regions between peach and almond. While we expected gene expression in fruit to overlap with candidate selected regions, instead we find enrichment for loci highly differentiated between the species, consistent with recent fossil evidence suggesting fruit divergence long preceded domestication. Taken together, this study tells us how closely related tree species evolve and are domesticated, the impact of these events on their genomes, and the utility of genomic information for long-lived species. Further exploration of this data will contribute to the genetic knowledge of these species and provide information regarding targets of selection for breeding application, and further the understanding of evolution in these species. Copyright © 2016 Velasco et al.

Evolutionary Genomics of Peach and Almond Domestication

Science.gov (United States)

Velasco, Dianne; Hough, Josh; Aradhya, Mallikarjuna; Ross-Ibarra, Jeffrey

2016-01-01

The domesticated almond [Prunus dulcis (L.) Batsch] and peach [P. persica (Mill.) D. A. Webb] originated on opposite sides of Asia and were independently domesticated ∼5000 yr ago. While interfertile, they possess alternate mating systems and differ in a number of morphological and physiological traits. Here, we evaluated patterns of genome-wide diversity in both almond and peach to better understand the impacts of mating system, adaptation, and domestication on the evolution of these taxa. Almond has around seven times the genetic diversity of peach, and high genome-wide FST values support their status as separate species. We estimated a divergence time of ∼8 MYA (million years ago), coinciding with an active period of uplift in the northeast Tibetan Plateau and subsequent Asian climate change. We see no evidence of a bottleneck during domestication of either species, but identify a number of regions showing signatures of selection during domestication and a significant overlap in candidate regions between peach and almond. While we expected gene expression in fruit to overlap with candidate selected regions, instead we find enrichment for loci highly differentiated between the species, consistent with recent fossil evidence suggesting fruit divergence long preceded domestication. Taken together, this study tells us how closely related tree species evolve and are domesticated, the impact of these events on their genomes, and the utility of genomic information for long-lived species. Further exploration of this data will contribute to the genetic knowledge of these species and provide information regarding targets of selection for breeding application, and further the understanding of evolution in these species. PMID:27707802

Assessment of maize stem borer damage on hybrid maize varieties in Chitwan, Nepal

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Buddhi Bahadur Achhami

2015-12-01

Full Text Available Maize is the second most important cereal crop in Nepal. However, national figure of grain production still remains below than the world's average grain production per unit area. Thus, this experiment was designed to determine the suitable time of maize planting, and to assess the peak period of one of the major insects, maize stem borer, in Chitwan condition. The results showed that plant damage percentage as per the maize planting month varies significantly, and the average plant damage percentage by stem borer was up to 18.11%. Length of the feeding tunnel in maize stem was significantly higher in January than July. In case of exit holes made by borer counted more than four holes per plant that were planted in the month of January. All in all, except the tunnel length measurement per plant, we observed similar pattern in other borer damage parameters such as exit whole counts and plant damage percentage within the tested varieties. Stem borer damage was not significantly affect on grain yield.

Rice stem borers in Malaya. A proposal to use mutation breeding for their control

Energy Technology Data Exchange (ETDEWEB)

Vohra, F C [University of Malaya, Kuala Lumpur (Malaysia)

1970-03-01

The problem of rice stem borers: Among the various problems in the rice crop the loss caused by the larvae of lepidopterous stem borers seems to be very serious. In some districts of the Kurian area, the rate of damage has been as much as 100%. It is not unlikely that the stem borer threat will become worse with the introduction of double cropping in many areas. The control of stem borers has mainly been through the application of various insecticides, namely DDT, BHC, Endrin, dieldrin and gamma-BHC, and this has met with a certain amount of success. The chemical method of control, however, is not fully satisfactory because of: (a) The high recurrent cost in application; (b) The danger of hazard to man by residues; (c) The toxic effects to fish and mammals in the paddy fields; (d) The poisoning of the natural parasites of the borers; (e) The difficulties for proper application, exact concentrations etc. by untrained farmers; and (f) The probability for development of insecticide resistance in stem borers. Apparently there is an urgent need for some control free from chemical hazards. Scientists in various countries have turned to breeding for varietal resistance, among other approaches, to save the crop from damage by stem borers. The development of a variety of rice resistant to stem borers will provide an effective control operative at all levels of insect population without additional cost and inconvenience to the farmer. The research project: To achieve this objective a research project has recently been started as a co-operative venture at the School of Biological Sciences, University of Malaya, Kuala Lumpur. This project involves (a) Detailed ecological studies of boxers in different varieties of rice grown commonly in the various parts of the country; (b) A study of parasites of borers for possible biological control; and (c) Production of rice varieties resistant to stem borers through induction of mutations and hybridization with locally used types

Densities of Agrilus auroguttatus and Other Borers in California and Arizona Oaks

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Laurel J. Haavik

2014-03-01

Full Text Available We investigated within-tree population density of a new invasive species in southern California, the goldspotted oak borer, Agrilus auroguttatus Schaeffer (Coleoptera: Buprestidae, with respect to host species and the community of other borers present. We measured emergence hole densities of A. auroguttatus and other borers on the lower stem (bole of naïve oaks at 18 sites in southern California and on co-evolved oaks at seven sites in southeastern Arizona. We sampled recently dead oaks in an effort to quantify the community of primary and secondary borers associated with mortality—species that were likely to interact with A. auroguttatus. Red oaks (Section Lobatae produced greater densities of A. auroguttatus than white oaks (Section Quercus. On red oaks, A. auroguttatus significantly outnumbered native borers in California (mean ± SE of 9.6 ± 0.7 versus 4.5 ± 0.6 emergence holes per 0.09 m2 of bark surface, yet this was not the case in Arizona (0.9 ± 0.2 versus 1.1 ± 0.2 emergence holes per 0.09 m2. In California, a species that is taxonomically intermediate between red and white oaks, Quercus chrysolepis (Section Protobalanus, exhibited similar A. auroguttatus emergence densities compared with a co-occurring red oak, Q. kelloggii. As an invasive species in California, A. auroguttatus may affect the community of native borers (mainly Buprestidae and Cerambycidae that feed on the lower boles of oaks, although it remains unclear whether its impact will be positive or negative.

Evaluation of heat treatment schedules for emerald ash borer (Coleoptera: Buprestidae).

Science.gov (United States)

Myers, Scott W; Fraser, Ivich; Mastro, Victor C

2009-12-01

The thermotolerance of the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), was evaluated by subjecting larvae and prepupae to a number of time-temperature regimes. Three independent experiments were conducted during 2006 and 2007 by heating emerald ash borer infested firewood in laboratory ovens. Heat treatments were established based on the internal wood temperature. Treatments ranged from 45 to 65 degrees C for 30 and 60 min, and the ability of larvae to pupate and emerge as adults was used to evaluate the success of each treatment. A fourth experiment was conducted to examine heat treatments on exposed prepupae removed from logs and subjected to ambient temperatures of 50, 55, and 60 degrees C for 15, 30, 45, and 60 min. Results from the firewood experiments were consistent in the first experiment. Emergence data showed emerald ash borer larvae were capable of surviving a temperatures-time combination up to 60 degrees C for 30 min in wood. The 65 degrees C for 30 min treatment was, however, effective in preventing emerald ash borer emergence on both dates. Conversely, in the second experiment using saturated steam heat, complete mortality was achieved at 50 and 55 degrees C for both 30 and 60 min. Results from the prepupae experiment showed emerald ash borer survivorship in temperature-time combinations up to 55 degrees C for 30 min, and at 50 degrees C for 60 min; 60 degrees C for 15 min and longer was effective in preventing pupation in exposed prepupae. Overall results suggest that emerald ash borer survival is variable depending on heating conditions, and an internal wood temperature of 60 degrees C for 60 min should be considered the minimum for safe treatment for firewood.

Emerald ash borer life cycle

Science.gov (United States)

Leah S. Bauer; Robert A. Haack; Deborah L. Miller; Toby R. Petrice; Houping Liu

2004-01-01

The emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), native to several Asian countries, was discovered in southeastern Michigan and nearby Ontario in June of 2002. EAB was identified as the cause of extensive ash (Fraxinus spp.) mortality in approximately 2,500 mi2, and...

Emerald Ash Borer (Coleoptera: Buprestidae)

Science.gov (United States)

The emerald ash borer, Agrilus planipennis Fairmaire, is an invasive beetle from Asia that has caused large scale ash (Fraxinus spp.) mortality in North America. This book chapter reviews the taxonomy, biology, life history of this invasive pest and its associated natural enemies in both its native ...

21 CFR 145.170 - Canned peaches.

Science.gov (United States)

2010-04-01

... CONSUMPTION CANNED FRUITS Requirements for Specific Standardized Canned Fruits § 145.170 Canned peaches. (a... by a support through which it can freely move upward or downward. The lower end of the rod is a plane...

A remarkable synergistic effect at the transcriptomic level in peach fruits doubly infected by prunus necrotic ringspot virus and peach latent mosaic viroid.

Science.gov (United States)

Herranz, Mari Carmen; Niehl, Annette; Rosales, Marlene; Fiore, Nicola; Zamorano, Alan; Granell, Antonio; Pallas, Vicente

2013-05-28

Microarray profiling is a powerful technique to investigate expression changes of large amounts of genes in response to specific environmental conditions. The majority of the studies investigating gene expression changes in virus-infected plants are limited to interactions between a virus and a model host plant, which usually is Arabidopsis thaliana or Nicotiana benthamiana. In the present work, we performed microarray profiling to explore changes in the expression profile of field-grown Prunus persica (peach) originating from Chile upon single and double infection with Prunus necrotic ringspot virus (PNRSV) and Peach latent mosaic viroid (PLMVd), worldwide natural pathogens of peach trees. Upon single PLMVd or PNRSV infection, the number of statistically significant gene expression changes was relatively low. By contrast, doubly-infected fruits presented a high number of differentially regulated genes. Among these, down-regulated genes were prevalent. Functional categorization of the gene expression changes upon double PLMVd and PNRSV infection revealed protein modification and degradation as the functional category with the highest percentage of repressed genes whereas induced genes encoded mainly proteins related to phosphate, C-compound and carbohydrate metabolism and also protein modification. Overrepresentation analysis upon double infection with PLMVd and PNRSV revealed specific functional categories over- and underrepresented among the repressed genes indicating active counter-defense mechanisms of the pathogens during infection. Our results identify a novel synergistic effect of PLMVd and PNRSV on the transcriptome of peach fruits. We demonstrate that mixed infections, which occur frequently in field conditions, result in a more complex transcriptional response than that observed in single infections. Thus, our data demonstrate for the first time that the simultaneous infection of a viroid and a plant virus synergistically affect the host transcriptome in

From observations to experiments in phenology research: investigating climate change impacts on trees and shrubs using dormant twigs.

Science.gov (United States)

Primack, Richard B; Laube, Julia; Gallinat, Amanda S; Menzel, Annette

2015-11-01

Climate change is advancing the leaf-out times of many plant species and mostly extending the growing season in temperate ecosystems. Laboratory experiments using twig cuttings from woody plant species present an affordable, easily replicated approach to investigate the relative importance of factors such as winter chilling, photoperiod, spring warming and frost tolerance on the leafing-out times of plant communities. This Viewpoint article demonstrates how the results of these experiments deepen our understanding beyond what is possible via analyses of remote sensing and field observation data, and can be used to improve climate change forecasts of shifts in phenology, ecosystem processes and ecological interactions. The twig method involves cutting dormant twigs from trees, shrubs and vines on a single date or at intervals over the course of the winter and early spring, placing them in containers of water in controlled environments, and regularly recording leaf-out, flowering or other phenomena. Prior to or following leaf-out or flowering, twigs may be assigned to treatment groups for experiments involving temperature, photoperiod, frost, humidity and more. Recent studies using these methods have shown that winter chilling requirements and spring warming strongly affect leaf-out and flowering times of temperate trees and shrubs, whereas photoperiod requirements are less important than previously thought for most species. Invasive plant species have weaker winter chilling requirements than native species in temperate ecosystems, and species that leaf-out early in the season have greater frost tolerance than later leafing species. This methodology could be extended to investigate additional drivers of leaf-out phenology, leaf senescence in the autumn, and other phenomena, and could be a useful tool for education and outreach. Additional ecosystems, such as boreal, southern hemisphere and sub-tropical forests, could also be investigated using dormant twigs to

Levels of control of Chilo partellus stem borer in segregating tropical ...

African Journals Online (AJOL)

In Kenya, stem borers destroy an estimated 400,000 metric tons, or 13.5%, of farmers' annual maize harvest costing about US$80 millions. Bacillus thuringiensis (Bt) maize controls stem borers without harming humans, livestock and the environment and was sown to 140m ha-1 globally in 2009. Two public Bt maize lines of ...

Guide to insect borers in North American broadleaf trees and shrubs

Science.gov (United States)

J.D. Solomon

1995-01-01

This book is an illustrated guide to 300 species of inset borers that attack hardwood trees, shrubs, and other woody angiosperms in North America. The major purposes of this guide are to identify insect borers and theri damage to provide information for controlling them. Readers most likely to find this guide useful are practiving foresters, entomologists, and others...

Emerald ash borer flight potential

Science.gov (United States)

Robin A. Taylor; Leah S. Bauer; Deborah L. Miller; Robert A. Haack

2005-01-01

The emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is an invasive pest of ash trees (Fraxinus spp.) that is rapidly spreading from the probable introduction site in Detroit, Michigan. The rapid spread to areas outside Michigan is undoubtedly due to phoretic transport on nursery stock, logs, and...

Emerald ash borer biological control

Science.gov (United States)

Leah Bauer; Juli Gould; Jian Duan; Mike. Ulyshen

2011-01-01

Emerald ash borer (EAB) (Agrilus planipennis), an invasive buprestid from northeast Asia, was identified in 2002 as the cause of ash (Fraxinus) tree mortality in southeast Michigan and adjacent areas of Ontario, Canada. This destructive beetle apparently arrived in North America via infested solid wood packaging materials from...

Genetic analysis identifies the region of origin of smuggled peach palm seeds.

Science.gov (United States)

Cristo-Araújo, Michelly; Molles, David Bronze; Rodrigues, Doriane Picanço; Clement, Charles R

2017-04-01

Seeds of a plant, supposedly a palm tree known popularly as peach palm (Bactris gasipaes), were seized by the Federal Police in the state of Pará, Brazil, without documentation of legal origin to authorize transportation and marketing in Brazil. They were alleged to be from the western part of Amazonas, Brazil, near the frontier with Peru and Colombia, justifying the lack of documentation. The species was confirmed to be peach palm. To determine the likely place of origin, a genetic analysis was performed to determine the relationship between the seized seeds and representative populations of peach palm from all of Amazonia, maintained in the Peach palm Core Collection, at the National Research Institute for Amazonia, using nine microsatellite loci. Reynolds' coancestry analysis showed a strong relationship between the seeds and the Pampa Hermosa landrace, around Yurimaguas, Peru. The Structure program, used to infer the probability of an individual belonging to a given population, showed that most seeds grouped with populations close to Yurimaguas, Peru, corroborating the coancestry analysis. The Pampa Hermosa landrace is the main source of spineless peach palm seeds used in the Brazilian heart-of-palm agribusiness, which motivated the smugglers to attempt this biopiracy. Copyright © 2017 Elsevier B.V. All rights reserved.

Essential Oil Composition of Pinus peuce Griseb. Needles and Twigs from Two National Parks of Kosovo.

Science.gov (United States)

Hajdari, Avni; Mustafa, Behxhet; Nebija, Dashnor; Selimi, Hyrmete; Veselaj, Zeqir; Breznica, Pranvera; Quave, Cassandra Leah; Novak, Johannes

The principal aim of this study was to analyze the chemical composition and qualitative and quantitative variability of essential oils obtained from seven naturally grown populations of the Pinus peuce Grisebach, Pinaceae in Kosovo. Plant materials were collected from three populations in the Sharri National Park and from four other populations in the Bjeshkët e Nemuna National Park, in Kosovo. Essential oils were obtained by steam distillation and analyzed by GC-FID (Gas Chromatography-Flame Ionization Detection) and GC-MS (Gas Chromatography-Mass Spectrometry). The results showed that the yield of essential oils (v/w dry weight) varied depending on the origin of population and the plant organs and ranged from 0.7 to 3.3%. In total, 51 compounds were identified. The main compounds were α-pinene (needles: 21.6-34.9%; twigs: 11.0-24%), β-phellandrene (needles: 4.1-27.7; twigs: 29.0-49.8%), and β-pinene (needles: 10.0-16.1; twigs: 6.9-20.7%). HCA (Hierarchical Cluster Analysis) and PCA (Principal Component Analyses) were used to assess geographical variations in essential oil composition. Statistical analysis showed that the analyzed populations are grouped in three main clusters which seem to reflect microclimatic conditions on the chemical composition of the essential oils.

Flavonoids from Twigs of Millettia leptobotrya Dunn.

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Zhi Na

2013-08-01

Full Text Available A new furanoisoflavone, 2 ' -methoxy-4 ' ,5 ' -methylenedioxy-[2 '' ,3 '' :7,8 ] furanoisoflavone, leptobotryanone ( 1 , and a new natural O-prenylated isoflavone, 4 ' -γ,γ-dimethylallyloxy-5,7-dihydroxyisoflavone ( 2 , were isolated from the twig s of Millettia leptobotrya, together with twelve known flavonoids, 4 ' -γ,γ-dimethylallyloxy-5-hydroxy-7-methoxyisoflavone ( 3 , 2 ' ,6,7-trimethoxy-4 ' ,5 ' -methylenedioxy- isoflavone (4, 2 ' ,7-dimethoxy-4 ' ,5 ' -methylenedioxyisoflavone (5, maximaisoflavone B (6, medicarpin (7 , maackiain (8, genistein (9, biochanin A (10, prunetin (11, chrysoeriol (12 , kaempferol (13 and desmoxyphyllin A (14 The structures of new compounds were elucidated on the basis of spectroscopic data interpretation , including 1D and 2D NMR and HREIMS. Th is is the first phytochemical investigation of this plant.

Xanthones from the twigs of Garcinia oblongifolia and their antidiabetic activity

DEFF Research Database (Denmark)

Trinh, Thi Dieu Binh; Quach, Tam T. T.; Bui, Dung N.

2017-01-01

Three new xanthones, oblongixanthone F-H ( 1 - 3 ), along with eight known xanthones ( 4 - 11 ), were isolated from an EtOAc extract of the twigs of Garcinia oblongifolia. Their structures were elucidated by spectroscopic analysis including 1D- and 2D-NMR spectroscopy and mass spectrometry. The a...

Hyperspectral imaging detection of decayed honey peaches based on their chlorophyll content.

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Sun, Ye; Wang, Yihang; Xiao, Hui; Gu, Xinzhe; Pan, Leiqing; Tu, Kang

2017-11-15

Honey peach is a very common but highly perishable market fruit. When pathogens infect fruit, chlorophyll as one of the important components related to fruit quality, decreased significantly. Here, the feasibility of hyperspectral imaging to determine the chlorophyll content thus distinguishing diseased peaches was investigated. Three optimal wavelengths (617nm, 675nm, and 818nm) were selected according to chlorophyll content via successive projections algorithm. Partial least square regression models were established to determine chlorophyll content. Three band ratios were obtained using these optimal wavelengths, which improved spatial details, but also integrates the information of chemical composition from spectral characteristics. The band ratio values were suitable to classify the diseased peaches with 98.75% accuracy and clearly show the spatial distribution of diseased parts. This study provides a new perspective for the selection of optimal wavelengths of hyperspectral imaging via chlorophyll content, thus enabling the detection of fungal diseases in peaches. Copyright © 2017 Elsevier Ltd. All rights reserved.

Impact of glutathione on the allergenicity of the peach lipid transfer protein Pru p 3.

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Gómez-Casado, C; Tordesillas, L; Kinkel, J; Starkl, P; Cuesta-Herranz, J; Roth-Walter F; Díaz-Perales, A; Jensen-Jarolim, E

2015-01-01

The allergenic potential of proteins can be altered under various physicochemical conditions. Glutathione (GSH) is a reducing agent that is used as an antioxidant in food products. We aimed to characterize the natural folding of peach proteins and test the allergenicity of reduced and natural Pru p 3, the major peach allergen. Pru p 3 was purified from peach, and its conformation was analyzed by means of circular dichroism. Using a thiol fluorescent probe, reduced proteins were detected in fresh peach. GSH-reduced Pru p 3 was tested in vitro for T-cell proliferation and in vivo using skin prick testing. GSH-reduced Pru p 3 produced variable skin prick reactions in peach-allergic patients. The proliferative response of peripheral blood mononuclear cells from allergic patients to reduced Pru p 3 tended to be less intense, whereas secretion of the cytokines IFN-γ, IL-5, and IL-10 was comparable. In a pool of sera from peach-allergic patients, reduction hardly impaired IgE-binding. Moreover, the stability of reduced Pru p 3 to gastrointestinal digestion was similar to that of the natural form. GSH can at least transiently reduce Pru p 3. We found that the effect of reduction on the allergenicity of Pru p 3 varied. Therefore, as an additive, GSH does not seem to eliminate the risk of reactions for peach-allergic patients.

[Genome-wide identification and expression analysis of the WRKY gene family in peach].

Science.gov (United States)

Gu, Yan-bing; Ji, Zhi-rui; Chi, Fu-mei; Qiao, Zhuang; Xu, Cheng-nan; Zhang, Jun-xiang; Zhou, Zong-shan; Dong, Qing-long

2016-03-01

The WRKY transcription factors are one of the largest families of transcriptional regulators and play diverse regulatory roles in biotic and abiotic stresses, plant growth and development processes. In this study, the WRKY DNA-binding domain (Pfam Database number: PF03106) downloaded from Pfam protein families database was exploited to identify WRKY genes from the peach (Prunus persica 'Lovell') genome using HMMER 3.0. The obtained amino acid sequences were analyzed with DNAMAN 5.0, WebLogo 3, MEGA 5.1, MapInspect and MEME bioinformatics softwares. Totally 61 peach WRKY genes were found in the peach genome. Our phylogenetic analysis revealed that peach WRKY genes were classified into three Groups: Ⅰ, Ⅱ and Ⅲ. The WRKY N-terminal and C-terminal domains of Group Ⅰ (group I-N and group I-C) were monophyletic. The Group Ⅱ was sub-divided into five distinct clades (groupⅡ-a, Ⅱ-b, Ⅱ-c, Ⅱ-d and Ⅱ-e). Our domain analysis indicated that the WRKY regions contained a highly conserved heptapeptide stretch WRKYGQK at its N-terminus followed by a zinc-finger motif. The chromosome mapping analysis showed that peach WRKY genes were distributed with different densities over 8 chromosomes. The intron-exon structure analysis revealed that structures of the WRKY gene were highly conserved in the peach. The conserved motif analysis showed that the conserved motifs 1, 2 and 3, which specify the WRKY domain, were observed in all peach WRKY proteins, motif 5 as the unknown domain was observed in group Ⅱ-d, two WRKY domains were assigned to GroupⅠ. SqRT-PCR and qRT-PCR results indicated that 16 PpWRKY genes were expressed in roots, stems, leaves, flowers and fruits at various expression levels. Our analysis thus identified the PpWRKY gene families, and future functional studies are needed to reveal its specific roles.

Tolerância de frutos de pessegueiro a geadas Fruit frost tolerance in peach

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André Paulo Assmann

2008-12-01

Full Text Available Este trabalho teve como objetivo avaliar a tolerância de frutos de pessegueiro aos danos ocasionados pela geada. A geada ocorreu no dia 5 de setembro de 2006, sendo que a temperatura mínima, a 1,5 m do solo, foi de - 1,06ºC. Foram avaliados 28 genótipos de pessegueiro em diferentes estádios fenológicos. O delineamento experimental foi o inteiramente casualizado, com três repetições (plantas de pessegueiro, procedendo-se à avaliação em seis ramos por planta. No dia em que ocorreu a geada, foram mensurados o número de frutos por ramo, diâmetro sutural médio dos frutos, número total de gemas vegetativas, percentual de brotação e percentual de brindilas formadas a partir das gemas brotadas. Uma segunda avaliação foi realizada 15 dias após a primeira, para avaliar o percentual de queda de frutos e o diâmetro sutural médio dos frutos remanescentes. Genótipos bem enfolhados e cujos frutos apresentavam endocarpo endurecido, no momento da ocorrência da geada, foram tolerantes ao dano ocasionado pelo frio; frutos com diâmetro sutural inferior a 20 mm foram suscetíveis à geada, enquanto frutos com diâmetro sutural superior a 30 mm apresentaram boa tolerância, independentemente do genótipo avaliado.The aim of this work was to evaluate the fruit damage tolerance in different peach tree genotypes after natural freeze. The frost happened on September 5, 2006, and the minimum temperature, 1.5 meters over soil, was -1.06ºC. Twenty- eight peach tree genotypes, in different stages of development, were evaluated, in a completely random design, in three replication (plants and six branches by plants. Immediately after frost, we measured the number of fruits per branches, average fruit suture diameter, percentage of sprouts and percentage of twigs in formation. A second evaluation was accomplished fifteen days after the first one in order to assess the percentage of fruit drop and the average remaining fruit suture diameter. Genotypes

Intake, digestibility and performance of lambs fed diets containing peach palm meal.

Science.gov (United States)

dos Santos, Alana Batista; Pereira, Mara Lúcia Albuquerque; de Oliveira Silva, Herymá Giovane; de Carvalho, Gleidson Giordano Pinto; de Jesus Pereira, Taiala Cristina; Ribeiro, Leandro Sampaio Oliveira; Azevêdo, José Augusto Gomes; das Graças Conceição Parada Costa Silva, Maria; Sousa, Larisse Borges; Sousa, Leandro Borges; de Oliveira Alencar, Daiane

2016-03-01

The aim of this study was to evaluate the intake and apparent digestibility of nutrients, performance, and plasma glucose concentration of ram lambs fed diets containing peach palm meal substituting maize (0, 10, 40, 60, and 85 % dry matter (DM)). Thirty Santa Inês rams with an average initial body weight of 21.6 ± 0.87 kg were distributed in a completely randomized design with five diets and six replicates. The substitution of the maize for the peach palm meal affected (P < 0.05) the intakes of DM, organic matter (OM), crude protein (CP), neutral detergent fiber corrected for ash and protein (NDFap), total carbohydrates (TC), total digestible nutrients (TDN), and metabolizable energy (ME), which decreased linearly (P < 0.05); the intake of ether extract (EE), however, fit an increasing linear equation (P < 0.05). The apparent digestibility coefficients of DM, OM, NDFap, and TC decreased linearly (P < 0.05) as the level of peach palm meal in the concentrate was increased. The total weight gain and the average daily gain decreased by 0.09 and 0.001 kg with each level of substitution of the maize for peach palm meal, respectively. It is recommended to substitute 40 % of the maize for peach palm meal.

Maize Cob Rot in Kenya and Its Association with Stalk Borer Damage

International Nuclear Information System (INIS)

Ajanga, S.I.

2002-01-01

Cob rots are a major cause of crop loss in areas such as western Kenya that experience prolonged rainfall during the period of crop maturation. Cob rot fungi cause spoilage of the grain and some of them produce mycotoxins which can pose a health risk to humans and animals consuming foods prepared from contaminate grain. survey conducted in western Kenya in 1998 showed that cob rot incidence exceeded 20%. In the following year when rainfall was greater around the harvest period, cob rot fungi affected 68% of cobs. in 1998 stalk borer larvae (mainly Busseola fusca) damaged 20% of the cobs and there was a strong correlation (R= 0.87) between cob rot incidence and borer damage. In 1999 almost half of the cobs sampled showed evidence of borer damage. The result indicate that the high cob rot incidence in this pert of Kenya is due to stalk bore damage, which predisposes the cobs to fungal infection, and that management of the borer would greatly decrease cob rot incidence

Mutation scanning of peach floral genes

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Wilde H Dayton

2011-05-01

Full Text Available Abstract Background Mutation scanning technology has been used to develop crop species with improved traits. Modifications that improve screening throughput and sensitivity would facilitate the targeted mutation breeding of crops. Technical innovations for high-resolution melting (HRM analysis are enabling the clinic-based screening for human disease gene polymorphism. We examined the application of two HRM modifications, COLD-PCR and QMC-PCR, to the mutation scanning of genes in peach, Prunus persica. The targeted genes were the putative floral regulators PpAGAMOUS and PpTERMINAL FLOWER I. Results HRM analysis of PpAG and PpTFL1 coding regions in 36 peach cultivars found one polymorphic site in each gene. PpTFL1 and PpAG SNPs were used to examine approaches to increase HRM throughput. Cultivars with SNPs could be reliably detected in pools of twelve genotypes. COLD-PCR was found to increase the sensitivity of HRM analysis of pooled samples, but worked best with small amplicons. Examination of QMC-PCR demonstrated that primary PCR products for further analysis could be produced from variable levels of genomic DNA. Conclusions Natural SNPs in exons of target peach genes were discovered by HRM analysis of cultivars from a southeastern US breeding program. For detecting natural or induced SNPs in larger populations, HRM efficiency can be improved by increasing sample pooling and template production through approaches such as COLD-PCR and QMC-PCR. Technical advances developed to improve clinical diagnostics can play a role in the targeted mutation breeding of crops.

Candidate gene database and transcript map for peach, a model species for fruit trees.

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Horn, Renate; Lecouls, Anne-Claire; Callahan, Ann; Dandekar, Abhaya; Garay, Lilibeth; McCord, Per; Howad, Werner; Chan, Helen; Verde, Ignazio; Main, Doreen; Jung, Sook; Georgi, Laura; Forrest, Sam; Mook, Jennifer; Zhebentyayeva, Tatyana; Yu, Yeisoo; Kim, Hye Ran; Jesudurai, Christopher; Sosinski, Bryon; Arús, Pere; Baird, Vance; Parfitt, Dan; Reighard, Gregory; Scorza, Ralph; Tomkins, Jeffrey; Wing, Rod; Abbott, Albert Glenn

2005-05-01

Peach (Prunus persica) is a model species for the Rosaceae, which includes a number of economically important fruit tree species. To develop an extensive Prunus expressed sequence tag (EST) database for identifying and cloning the genes important to fruit and tree development, we generated 9,984 high-quality ESTs from a peach cDNA library of developing fruit mesocarp. After assembly and annotation, a putative peach unigene set consisting of 3,842 ESTs was defined. Gene ontology (GO) classification was assigned based on the annotation of the single "best hit" match against the Swiss-Prot database. No significant homology could be found in the GenBank nr databases for 24.3% of the sequences. Using core markers from the general Prunus genetic map, we anchored bacterial artificial chromosome (BAC) clones on the genetic map, thereby providing a framework for the construction of a physical and transcript map. A transcript map was developed by hybridizing 1,236 ESTs from the putative peach unigene set and an additional 68 peach cDNA clones against the peach BAC library. Hybridizing ESTs to genetically anchored BACs immediately localized 11.2% of the ESTs on the genetic map. ESTs showed a clustering of expressed genes in defined regions of the linkage groups. [The data were built into a regularly updated Genome Database for Rosaceae (GDR), available at (http://www.genome.clemson.edu/gdr/).].

Effect of Particle Orientation during Thermal Processing of Canned Peach Halves: A CFD Simulation

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Adreas Dimou

2014-05-01

Full Text Available The objective of this work was to apply Computational Fluid Dynamics (CFD to study the effect of particle orientation on fluid flow, temperature evolution, as well as microbial destruction, during thermal processing of still cans filled with peach halves in sugar syrup. A still metal can with four peach halves in 20% sugar syrup was heated at 100 °C for 20 min and thereafter cooled at 20 °C. Infinite heat transfer coefficient between heating medium and external can wall was considered. Peach halves were orderly placed inside the can with the empty space originally occupied by the kernel facing, in all peaches, either towards the top or the bottom of the can. In a third situation, the can was placed horizontally. Simulations revealed differences on particle temperature profiles, as well as process F values and critical point location, based on their orientation. At their critical points, peach halves with the kernel space facing towards the top of the can heated considerably slower and cooled faster than the peaches having their kernel space facing towards the bottom of the can. The horizontal can case exhibited intermediate cooling but the fastest heating rates and the highest F process values among the three cases examined. The results of this study could be used in designing of thermal processes with optimal product quality.

Occurrence of emerald ash borer (Coleoptera: Buprestidae) and biotic factors affecting its immature stages in the Russian Far East.

Science.gov (United States)

Duan, Jian J; Yurchenko, Galina; Fuester, Roger

2012-04-01

Field surveys were conducted from 2008 to 2011 in the Khabarovsk and Vladivostok regions of Russia to investigate the occurrence of emerald ash borer, Agrilus planipennis Fairmaire, and mortality factors affecting its immature stages. We found emerald ash borer infesting both introduced North American green ash (Fraxinus pennsylvanica Marshall) and native oriental ashes (F. mandshurica Rupr. and F. rhynchophylla Hance) in both regions. Emerald ash borer densities (larvae/m(2) of phloem area) were markedly higher on green ash (11.3-76.7 in the Khabarovsk area and 77-245 in the Vladivostok area) than on artificially stressed Manchurian ash (2.2) or Oriental ash (10-59). Mortality of emerald ash borer larvae caused by different biotic factors (woodpecker predation, host plant resistance and/or undetermined diseases, and parasitism) varied with date, site, and ash species. In general, predation of emerald ash borer larvae by woodpeckers was low. While low rates (3-27%) of emerald ash borer larval mortality were caused by undetermined biotic factors on green ash between 2009 and 2011, higher rates (26-95%) of emerald ash borer larval mortality were caused by putative plant resistance in Oriental ash species in both regions. Little (emerald ash borer larvae was observed in Khabarovsk; however, three hymenopteran parasitoids (Spathius sp., Atanycolus nigriventris Vojnovskaja-Krieger, and Tetrastichus planipennisi Yang) were observed attacking third - fourth instars of emerald ash borer in the Vladivostok area, parasitizing 0-8.3% of emerald ash borer larvae infesting Oriental ash trees and 7.3-62.7% of those on green ash trees (primarily by Spathius sp.) in two of the three study sites. Relevance of these findings to the classical biological control of emerald ash borer in newly invaded regions is discussed.

Biotic and abiotic factors affect green ash volatile production and emerald ash borer adult feeding preference.

Science.gov (United States)

Chen, Yigen; Poland, Therese M

2009-12-01

The emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is an exotic woodborer first detected in 2002 in Michigan and Ontario and is threatening the ash resource in North America. We examined the effects of light exposure and girdling on green ash (Fraxinus pennsylvanica Marsh) volatile production, and effects of light exposure, girdling, and leaf age on emerald ash borer adult feeding preferences and phototaxis. Green ash seedlings grown under higher light exposure had lower amounts of three individual volatile compounds, (Z)-3-hexenol, (E)-beta-ocimene, and (Z,E)-alpha-farnesene, as well as the total amount of six detected volatile compounds. Girdling did not affect the levels of these volatiles. Emerald ash borer females preferred mature leaves, leaves from girdled trees, and leaves grown in the sun over young leaves, leaves from nongirdled trees, and leaves grown in the shade, respectively. These emerald ash borer preferences were most likely because of physical, nutritional, or biochemical changes in leaves in response to the different treatments. Emerald ash borer females and males showed positive phototaxis in laboratory arenas, a response consistent with emerald ash borer preference for host trees growing in sunlight.

Laboratory rearing of emerald ash borer

Science.gov (United States)

Leah S. Bauer; Robert A. Haack; Deborah L. Miller; Houping Liu; Toby Petrice

2004-01-01

The emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), native to several Asian countries, was identified in 2002 as the cause of ash (Fraxinus spp.) mortality throughout southeastern Michigan and southwestern Ontario. More isolated infestations continue to be found throughout Lower Michigan, northern...

Essential Oil Composition of Pinus peuce Griseb. Needles and Twigs from Two National Parks of Kosovo

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Avni Hajdari

2016-01-01

Full Text Available The principal aim of this study was to analyze the chemical composition and qualitative and quantitative variability of essential oils obtained from seven naturally grown populations of the Pinus peuce Grisebach, Pinaceae in Kosovo. Plant materials were collected from three populations in the Sharri National Park and from four other populations in the Bjeshkët e Nemuna National Park, in Kosovo. Essential oils were obtained by steam distillation and analyzed by GC-FID (Gas Chromatography-Flame Ionization Detection and GC-MS (Gas Chromatography-Mass Spectrometry. The results showed that the yield of essential oils (v/w dry weight varied depending on the origin of population and the plant organs and ranged from 0.7 to 3.3%. In total, 51 compounds were identified. The main compounds were α-pinene (needles: 21.6–34.9%; twigs: 11.0–24%, β-phellandrene (needles: 4.1–27.7; twigs: 29.0–49.8%, and β-pinene (needles: 10.0–16.1; twigs: 6.9–20.7%. HCA (Hierarchical Cluster Analysis and PCA (Principal Component Analyses were used to assess geographical variations in essential oil composition. Statistical analysis showed that the analyzed populations are grouped in three main clusters which seem to reflect microclimatic conditions on the chemical composition of the essential oils.

Measuring the impact of biotic factors on populations of immature emerald ash borers (Coleoptera: Buprestidae).

Science.gov (United States)

Duan, Jian J; Ulyshen, Michael D; Bauer, Leah S; Gould, Juli; Van Driesche, Roy

2010-10-01

Cohorts of emerald ash borer larvae, Agrilus planipennis Fairmaire, were experimentally established in July of 2008 on healthy green ash (Fraxinus pennsylvanica) trees in two wooded plots at each of three sites near Lansing, MI, by caging gravid emerald ash borer females or placing laboratory-reared eggs on trunks (0.5-2 m above the ground) of selected trees. One plot at each site was randomly chosen for release of two introduced larval parasitoids, Tetrastichus planipennisi Yang (Hymenoptera: Eulophidae) and Spathius agrili Yang (Hymenoptera: Braconidae), whereas the other served as the control. Stage-specific mortality factors and rates were measured for all experimentally established cohorts and for associated wild (i.e., naturally occurring) emerald ash borer immature stages via destructive sampling of 2.5 m (above the ground) trunk sections of cohort-bearing trees in the spring and fall of 2009. Host tree defense was the most important mortality factor, causing 32.0 to 41.1% mortality in the experimental cohorts and 17.5 to 21.5% in wild emerald ash borer stages by spring 2009, and 16.1 to 29% for the remaining experimental cohorts, and 9.9 to 11.8% for wild immature emerald ash borer stages by fall 2009. Woodpecker predation was the second most important factor, inflicting no mortality in the experimental cohorts but causing 5.0 to 5.6% mortality to associated wild emerald ash borer stages by spring 2009 and 9.2 to 12.8% and 3.2 to 17.7%, respectively, for experimental cohorts and wild emerald ash borer stages by fall 2009. Mortality from disease in both the experimental and wild cohorts was low (emerald ash borer stages were parasitized by T. planipennisi. While there were no significant differences in mortality rates because of parasitism between parasitoid-release and control plots, T. planipennisi was detected in each of the three release sites by the end of the study but was not detected in the experimental cohorts or associated wild larvae in any of the

A New Phenyl Ethyl Glycoside from the Twigs of Acer tegmentosum.

Science.gov (United States)

Park, Seonju; Lee, Hwa Young; Nhiem, Nguyen Xuan; Lee, Taek Hwan; Kim, Nanyoung; Cho, Seung Hun; Kim, Seung Hyun

2015-07-01

One new phenyl ethyl glycoside, 2-(4-hydroxyphenyl)ethyl-O-α-L-arabinofuranosyl-(1 --> 6)-O-β-D-glucopyranoide (1) and 11 known compounds (2-12) were isolated from the twigs of Acer tegmentosum. Compound 6 showed potent anti-neuroinflammatory activity against the LPS-stimulated BV-2 microglial cells with tNO production of 25.0 ± 2.5 μM and TNF-α concentration of 617.6 ± 47.1 pg/mL at 30 μM.

Somatic Embryogenesis in Peach-Palm (Bactris gasipaes) Using Different Explant Sources.

Science.gov (United States)

Steinmacher, Douglas A; Heringer, Angelo Schuabb; Jiménez, Víctor M; Quoirin, Marguerite G G; Guerra, Miguel P

2016-01-01

Peach palm (Bactris gasipaes Kunth) is a member of the family Arecaceae and is a multipurpose but underutilized species. Nowadays, fruit production for subsistence and local markets, and heart-of-palm production for local, national, and international markets are the most important uses of this plant. Conventional breeding programs in peach palm are long-term efforts due to the prolonged generation time, large plant size, difficulties with controlled pollination and other factors. Although it is a caespitose palm, its propagation is currently based on seeds, as off-shoots are difficult to root. Hence, tissue culture techniques are considered to be the most likely strategy for efficient clonal plantlet regeneration of this species. Among various techniques, somatic embryogenesis offers the advantages of potential automated large-scale production and putative genetic stability of the regenerated plantlets. The induction of somatic embryogenesis in peach palm can be achieved by using different explant sources including zygotic embryos, immature inflorescences and thin cell layers from the young leaves and shoot meristems. The choice of a particular explant depends on whether clonal propagation is desired or not, as well as on the plant conditions and availability of explants. Protocols to induce and express somatic embryogenesis from different peach palm explants, up to acclimatization of plantlets, are described in this chapter.

A Non-Targeted Approach Unravels the Volatile Network in Peach Fruit

Science.gov (United States)

Sánchez, Gerardo; Besada, Cristina; Badenes, María Luisa; Monforte, Antonio José; Granell, Antonio

2012-01-01

Volatile compounds represent an important part of the plant metabolome and are of particular agronomic and biological interest due to their contribution to fruit aroma and flavor and therefore to fruit quality. By using a non-targeted approach based on HS-SPME-GC-MS, the volatile-compound complement of peach fruit was described. A total of 110 volatile compounds (including alcohols, ketones, aldehydes, esters, lactones, carboxylic acids, phenolics and terpenoids) were identified and quantified in peach fruit samples from different genetic backgrounds, locations, maturity stages and physiological responses. By using a combination of hierarchical cluster analysis and metabolomic correlation network analysis we found that previously known peach fruit volatiles are clustered according to their chemical nature or known biosynthetic pathways. Moreover, novel volatiles that had not yet been described in peach were identified and assigned to co-regulated groups. In addition, our analyses showed that most of the co-regulated groups showed good intergroup correlations that are therefore consistent with the existence of a higher level of regulation orchestrating volatile production under different conditions and/or developmental stages. In addition, this volatile network of interactions provides the ground information for future biochemical studies as well as a useful route map for breeding or biotechnological purposes. PMID:22761719

Low dose UV and gamma radiation on storage rot and physicochemical changes in peaches

International Nuclear Information System (INIS)

Lu, J.Y.; Lukombo, S.M.; Stevens, C.; Khan, V.A.; Wilson, C.L.; Pusey, P.L.; Chaultz, E.

1993-01-01

Peach fruit were irradiated with 7.5 x 10(4) ergs/mm(2) of UV (254nm) or 0.1 kGy gamma rays or a combination of both, then stored at 16C for 21 days. The results showed that both UV and gamma rays reduced storage rot and delayed ripening. UV treated peaches had lower sugar concentration, total phenols, anthocyanins and lower weight loss than the gamma treated peaches. The combination of UV and gamma showed no advantage over the use of UV or gamma alone

Economic analysis of emerald ash borer (Coleoptera: Buprestidae) management options.

Science.gov (United States)

Vannatta, A R; Hauer, R H; Schuettpelz, N M

2012-02-01

Emerald ash borer, Agrilus planipennis (Fairmaire) (Coleoptera: Buprestidae), plays a significant role in the health and extent of management of native North American ash species in urban forests. An economic analysis of management options was performed to aid decision makers in preparing for likely future infestations. Separate ash tree population valuations were derived from the i-Tree Streets program and the Council of Tree and Landscape Appraisers (CTLA) methodology. A relative economic analysis was used to compare a control option (do-nothing approach, only removing ash trees as they die) to three distinct management options: 1) preemptive removal of all ash trees over a 5 yr period, 2) preemptive removal of all ash trees and replacement with comparable nonash trees, or 3) treating the entire population of ash trees with insecticides to minimize mortality. For each valuation and management option, an annual analysis was performed for both the remaining ash tree population and those lost to emerald ash borer. Retention of ash trees using insecticide treatments typically retained greater urban forest value, followed by doing nothing (control), which was better than preemptive removal and replacement. Preemptive removal without tree replacement, which was the least expensive management option, also provided the lowest net urban forest value over the 20-yr simulation. A "no emerald ash borer" scenario was modeled to further serve as a benchmark for each management option and provide a level of economic justification for regulatory programs aimed at slowing the movement of emerald ash borer.

Biology of emerald ash borer parasitoids

Science.gov (United States)

Leah S. Bauer; Jian J. Duan; Jonathan P. Lelito; Houping Liu; Juli R. Gould

2015-01-01

The emerald ash borer (EAB) (Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), an invasive beetle introduced from China (Bray et al., 2011), was identified as the cause of ash (Fraxinus spp.) mortality in southeast Michigan and nearby Ontario in 2002 (Haack et al., 2002; Federal Register, 2003; Cappaert et al., 2005)....

Phenotypic characterization of qualitative parameters and antioxidant contents in peach and nectarine fruit and changes after jam preparation.

Science.gov (United States)

Drogoudi, Pavlina; Gerasopoulos, Dimitrios; Kafkaletou, Mina; Tsantili, Eleni

2017-08-01

Sugars and antioxidants in peaches contribute to fresh fruit quality and nutrition; however, information on widely grown cultivars and changes induced after peach jam preparation is limited. In the present study, colour, sugars and antioxidant parameters were determined in fruit and jam from 45 peach and nectarine cultivars. Pronounced varietal differences were found in sorbitol (42-fold range), total phenolics (TPs) and antioxidant capacities (10- to 19-fold range). Sorbitol levels were greater in non-melting peach, followed by nectarine, and lower values were found in melting peach cultivars. Late-harvested peach and nectarine cultivars tended to have a higher soluble solid content and antioxidant potential. Cultivars with relatively high antioxidant contents produced darker and redder jams, containing more antioxidants, than the jam or the fruit from the other cultivars. Jam-TPs were reduced by 48% compared to fruit-TPs, with greater reduction being noted in high antioxidant cultivars. The most favorable jam organoleptic characteristics were found in 'Morsiani 90', 'Amiga', 'Romea' and 'Alirosada', as well as in non-melting compared to melting peach cultivars. The best cultivars for each fruit flesh type and jam were identified. Peach jam could be an alternative substitute when fresh fruit is not available and when it is prepared with high antioxidant cultivars. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

Occupational Allergy to Peach (Prunus persica) Tree Pollen and Potential Cross-Reactivity between Rosaceae Family Pollens.

Science.gov (United States)

Jiang, Nannan; Yin, Jia; Mak, Philip; Wen, Liping

2015-10-01

Orchard workers in north China are highly exposed to orchard pollens, especially peach and other Rosaceae family pollens during pollination season. The aim of this study was to investigate whether occupational allergy to peach tree pollen as a member of Rosaceae family is IgE-mediated and to evaluate the cross-reactivity among Rosaceae family pollens. Allergen skin test and conjunctival challenge test were performed; enzyme linked immune-sorbent assay (ELISA), inhibiting ELISA, western immunoblotting and inhibiting western immunoblotting were done with Rosaceae family orchard pollens, including peach, apricot, cherry, apple and pear tree pollens. Mass spectrometry was also performed to probe the main allergen component and cross-reactive protein. Sensitizations to peach pollen were found in both skin test and conjunctival challenge in the patients. Serum specific IgE to three pollens (peach, apricot and cherry) were detected through ELISA. When peach pollen used as solid phase, ELISA inhibition revealed other four kinds of pollens capable of inducing partial to strong inhibitions (45% to 87%), with the strongest inhibition belonging to apricot pollen (87%). Western blotting showed predominant IgE binding to a 20 KD protein among these pollens, which appeared to be a cross-reactive allergen component through western blotting inhibition. It was recognized as a protein homologous to glutathione s-transferase 16 from Arabidopsis thaliana. Peach and other Rosaceae family tree pollen may serve as a potential cause of IgE mediated occupational respiratory disease in orchard workers in north China.

Effects of infrared radiation heating on peeling performance and quality attributes of clingstone peaches

Science.gov (United States)

Salinity and wastewater disposal problems associated with the conventional wet-lye method for peeling clingstone peaches result in considerable negative environment impacts. This study investigated the efficacy of using infrared (IR) heating as an alternative method for peach peel removal without us...

Assessing the fatty acid, essential oil composition, their radical scavenging and antibacterial activities of Schinus terebinthifolius Raddi leaves and twigs.

Science.gov (United States)

Ennigrou, Asma; Casabianca, Hervé; Vulliet, Emmanuelle; Hanchi, Belgacem; Hosni, Karim

2018-04-01

The fatty acid, essential oil compositions and their respective antioxidant and antibacterial activities was determined in Schinus terebinthifolius Raddi leaves and twigs. The lipid content ranged from 1.75 to 4.65% in twigs and leaves, respectively. Thirteen fatty acids were identified with α-linolenic (C18:3), palmitic (C16:0) and linoleic (C18:2) acids being the main components. The essential oils of both organs were characterized by a high amount of monoterpene hydrocarbons (68.91-74.88%) with α-phellandrene (33.06-36.18%), α-pinene (14.85-15.18%) and limonene (6.62-8.79%) being the chief components. The DPPH˙ radical scavenging assay revealed that both oils have a very weak antiradical activity. In contrast, they showed an appreciable antibacterial activity against the gram-positive Enterococcus feacium (ATCC 19434) and Streptococcus agalactiae (ATCC 13813) bacteria. These results suggest that leaves and twigs of S. terebinthifolius could be considered as an important dietary source of health promoting phytochemicals and has a good potential for use in food industry and pharmacy.

Emerald ash borer genetics: an update

Science.gov (United States)

Alicia M. Bray; Leah S. Bauer; Robert A. Haack; Therese Poland; James J. Smith

2008-01-01

Emerald ash borer (EAB), Agrilus planipennis Fairmaire, samples were collected from introduced sites in Michigan, Ohio, Indiana, Pennsylvania, Illinois, and Ontario, Canada, as well as native sites in China, Japan, and South Korea with the help of a network of collaborators. The beetles were analyzed using DNA sequences from mitochondrial cytochrome...

Effectiveness of differing trap types for the detection of emerald ash borer (Coleoptera: Buprestidae).

Science.gov (United States)

Marshall, Jordan M; Storer, Andrew J; Fraser, Ivich; Beachy, Jessica A; Mastro, Victor C

2009-08-01

The early detection of populations of a forest pest is important to begin initial control efforts, minimizing the risk of further spread and impact. Emerald ash borer (Agrilus planipennis Fairmaire) is an introduced pestiferous insect of ash (Fraxinus spp. L.) in North America. The effectiveness of trapping techniques, including girdled trap trees with sticky bands and purple prism traps, was tested in areas with low- and high-density populations of emerald ash borer. At both densities, large girdled trap trees (>30 cm diameter at breast height [dbh], 1.37 m in height) captured a higher rate of adult beetles per day than smaller trees. However, the odds of detecting emerald ash borer increased as the dbh of the tree increased by 1 cm for trap trees 15-25 cm dbh. Ash species used for the traps differed in the number of larvae per cubic centimeter of phloem. Emerald ash borer larvae were more likely to be detected below, compared with above, the crown base of the trap tree. While larval densities within a trap tree were related to the species of ash, adult capture rates were not. These results provide support for focusing state and regional detection programs on the detection of emerald ash borer adults. If bark peeling for larvae is incorporated into these programs, peeling efforts focused below the crown base may increase likelihood of identifying new infestations while reducing labor costs. Associating traps with larger trees ( approximately 25 cm dbh) may increase the odds of detecting low-density populations of emerald ash borer, possibly reducing the time between infestation establishment and implementing management strategies.

Emerald ash borer survival in firewood

Science.gov (United States)

Robert A. Haack; Toby R. Petrice

2005-01-01

The emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is native to Asia and was first discovered in Michigan and Ontario in 2002. As of October 2004, EAB was only found to breed in ash (Fraxinus) trees in North America. EAB is spreading naturally through adult flight as well as artificially through...

Infestation of the banana root borer among different banana plant genotypes

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Fernando Teixeira de Oliveira

Full Text Available ABSTRACT: In this study, we aimed to investigate Cosmopolites sordidus (Coleoptera: Dryophthoridae infestation among different banana genotypes in a commercial banana orchard over the course of 30 months. Banana root borer infestation was compared in 20 banana genotypes, including five varieties and 15 hybrids. Overall, we observed that 94.17% of pest infestation cases occurred in the cortex region, and only 5.83% occurred in the central cylinder. Genotypes least sensitive to infestation were the Prata Anã (AAB and Pacovan (AAB varieties, where no damage was recorded. Among the hybrid genotypes, PV 9401 and BRS Fhia 18 showed intermediate levels of sensitivity, while BRS Tropical hybrids (AAAB, PA 9401 (AAAB, BRS Vitoria (AAAB, YB 4203 (AAAB, and Bucaneiro (AAAA were the most sensitive to attack by banana root borer. This study demonstrated that the infestation of the banana root borer varies according banana plant genotype, and the utilization of less susceptible genotypes could reduce infestation rates of C. sordidus.

Silage or fresh by-product of peach palm as roughage in the feeding of lambs.

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dos Santos Cabral, Ícaro; Azevêdo, José Augusto Gomes; de Almeida, Flávio Moreira; Pereira, Luiz Gustavo Ribeiro; de Araújo, Gherman Garcia Leal; Nogueira, Abdon Santos; Souza, Lígia Lins; de Oliveira, Gisele Andrade; de Oliveira Filho, Carlos Alberto Alves

2015-03-01

The objective of this study was to evaluate intake and apparent digestibility of agro-industrial by-product of peach palm in diets for lambs. Twenty castrated, crossbred Santa Ines lambs, with average age of 150 days and body weight of 22.4 ± 3.4 kg, were distributed in a completely randomized design with four experimental diets composed of the following: fresh by-product of peach palm enriched with urea + ammonia sulfate (FU); fresh peach palm by-product + concentrate (FP); silage of peach palm by-product + concentrate (SP); and silage of peach palm by-product enriched with 15% of cornmeal + concentrate (SPC). Intake was recorded daily, and the digestibility coefficients were estimated with the internal marker indigestible acid detergent fiber (iADF). Diet FU resulted in the lowest intake and digestibility of the nutrients evaluated. Animals receiving diet FP showed higher intakes of dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fiber (NDF), total digestible nutrients (TDN), and digestible energy (DE) in relation to animals fed diets SP and SPC. Diets SP and SPC showed higher coefficients of digestibility of DM, OM, CP, and NDF than diet FP. Diet SP reduced the intakes of DM, OM, ether extract (EE), non-fibrous carbohydrate (NFC), TDN, and DE and the digestibility coefficients of DM, OM, and NFC as compared with diet SPC. Feedlot lambs fed a diet with fresh peach palm by-product + concentrate (diet FP) have higher nutrient intake.

Investigation into effects of ionizing radiations on physical-chemical properties of bulgarian sorts of peaches and grapes

International Nuclear Information System (INIS)

Dimitrov, D.; Kalinov, V.; Nikolova, M.; Pavlova, E.

1974-01-01

The aim of this study was to ascertain the degree and direction of those changes in physicochemical characteristics of irradiated peaches and grapes which determine their quality after irradiation and during storage. The following main conclusions are made: irradiation with doses of 200 to 300 krad does not cause significant alterations in the chemical composition and physicochemical characteristics of peaches and grapes; irradiation has a direct effect on the consistancy of peaches and grapes, leading to their softening which is proportional to the radiation dose used; radiation treatment is advisable in the case of peaches and grapes in tended for short-term rather than long-term storage at low temperatures

Do rice water weevils and rice stem borers compete when sharing a host plant?

Science.gov (United States)

Shi, Sheng-Wei; He, Yan; Ji, Xiang-Hua; Jiang, Ming-Xing; Cheng, Jia-An

2008-07-01

The rice water weevil (RWW) Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae) is an invasive insect pest of rice Oryza sativa L. in China. Little is known about the interactions of this weevil with indigenous herbivores. In the present study, adult feeding and population density of the weevil, injury level of striped stem borer Chilo suppressalis (Walker) (Lepidoptera: Pyralidae) and pink stem borer Sesamia inferens (Walker) (Lepidoptera: Noctuidae) to rice, as well as growth status of their host plants were surveyed in a rice field located in Southeastern Zhejiang, China, in 2004 with the objective to discover interspecific interactions on the rice. At tillering stage, both adult feeding of the weevil and injury of the stem borers tended to occur on larger tillers (bearing 5 leaves) compared with small tillers (bearing 2~4 leaves), but the insects showed no evident competition with each other. At booting stage, the stem borers caused more withering/dead hearts and the weevil reached a higher density on the plants which had more productive tillers and larger root system; the number of weevils per tiller correlated negatively with the percentage of withering/dead hearts of plants in a hill. These observations indicate that interspecific interactions exist between the rice water weevil and the rice stem borers with negative relations occurring at booting or earlier developmental stages of rice.

Do rice water weevils and rice stem borers compete when sharing a host plant?*

Science.gov (United States)

Shi, Sheng-wei; He, Yan; Ji, Xiang-hua; Jiang, Ming-xing; Cheng, Jia-an

2008-01-01

The rice water weevil (RWW) Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae) is an invasive insect pest of rice Oryza sativa L. in China. Little is known about the interactions of this weevil with indigenous herbivores. In the present study, adult feeding and population density of the weevil, injury level of striped stem borer Chilo suppressalis (Walker) (Lepidoptera: Pyralidae) and pink stem borer Sesamia inferens (Walker) (Lepidoptera: Noctuidae) to rice, as well as growth status of their host plants were surveyed in a rice field located in Southeastern Zhejiang, China, in 2004 with the objective to discover interspecific interactions on the rice. At tillering stage, both adult feeding of the weevil and injury of the stem borers tended to occur on larger tillers (bearing 5 leaves) compared with small tillers (bearing 2~4 leaves), but the insects showed no evident competition with each other. At booting stage, the stem borers caused more withering/dead hearts and the weevil reached a higher density on the plants which had more productive tillers and larger root system; the number of weevils per tiller correlated negatively with the percentage of withering/dead hearts of plants in a hill. These observations indicate that interspecific interactions exist between the rice water weevil and the rice stem borers with negative relations occurring at booting or earlier developmental stages of rice. PMID:18600788

The desorption of caesium from Peach Bottom HTGR steam generator materials

International Nuclear Information System (INIS)

Clark, M.J.

1979-03-01

The work at Harwell on the Peach Bottom End-of-Life Program in co-operation with the General Atomic Company (U.S.A.) is described. Materials taken from the Economiser, Evaporator and Superheater Sections of the Peach Bottom Unit No. 1. High Temperature Gas Cooled Reactor (HTGR) Heat Exchanger were placed in a reducing atmosphere comparable to the composition of an HTGR helium coolant gas, and the desorption of caesium isotopes measured under known conditions of flow, temperature and oxygen pressure. (author)

Difference in photosynthetic performance among three peach ...

African Journals Online (AJOL)

The effects of low photosynthetic photon flux density (PPFD) on greenhouse grown peach trees ('Qingfeng': Prunus persica L. Batsch, 'NJN76': Prunus persica L. Batsch and 'Maixiang': P. persica var. nectarine) were investigated. Difference in photosynthesis rate (Pn) and stoma morphology among cultivars were studied.

Failure to phytosanitize ash firewood infested with emerald ash borer in a small dry kiln using ISPM-15 standards.

Science.gov (United States)

Goebel, P Charles; Bumgardner, Matthew S; Herms, Daniel A; Sabula, Andrew

2010-06-01

Although current USDA-APHIS standards suggest that a core temperature of 71.1 degrees C (160 degrees F) for 75 min is needed to adequately sanitize emerald ash borer, Agrilus planipennis Fairmaire-infested firewood, it is unclear whether more moderate (and economical) treatment regimes will adequately eradicate emerald ash borer larvae and prepupae from ash firewood. We constructed a small dry kiln in an effort to emulate the type of technology a small- to medium-sized firewood producer might use to examine whether treatments with lower temperature and time regimes successfully eliminate emerald ash borer from both spilt and roundwood firewood. Using white ash (Fraxinus americana L.) firewood collected from a stand with a heavy infestation of emerald ash borer in Delaware, OH, we treated the firewood using the following temperature and time regime: 46 degrees C (114.8 degrees F) for 30 min, 46 degrees C (114.8 degrees F) for 60 min, 56 degrees C (132.8 degrees F) for 30 min, and 56 degrees C (132.8 degrees F) for 60 min. Temperatures were recorded for the outer 2.54-cm (1-in.) of firewood. After treatment, all firewood was placed under mesh netting and emerald ash borer were allowed to develop and emerge under natural conditions. No treatments seemed to be successful at eliminating emerald ash borer larvae and perpupae as all treatments (including two nontreated controls) experienced some emerald ash borer emergence. However, the 56 degrees C (132.8 degrees F) treatments did result in considerably less emerald ash borer emergence than the 46 degrees C (114.8 degrees F) treatments. Further investigation is needed to determine whether longer exposure to the higher temperature (56 degrees C) will successfully sanitize emerald ash borer-infested firewood.

Analysis of core damage frequency from internal events: Peach Bottom, Unit 2

International Nuclear Information System (INIS)

Kolaczkowski, A.M.; Lambright, J.A.; Ferrell, W.L.; Cathey, N.G.; Najafi, B.; Harper, F.T.

1986-10-01

This document contains the internal event initiated accident sequence analyses for Peach Bottom, Unit 2; one of the reference plants being examined as part of the NUREG-1150 effort by the Nuclear Regulatory Commission. NUREG-1150 will document the risk of a selected group of nuclear power plants. As part of that work, this report contains the overall core damage frequency estimate for Peach Bottom, Unit 2, and the accompanying plant damage state frequencies. Sensitivity and uncertainty analyses provided additional insights regarding the dominant contributors to the Peach Bottom core damage frequency estimate. The mean core damage frequency at Peach Bottom was calculated to be 8.2E-6. Station blackout type accidents (loss of all ac power) were found to dominate the overall results. Anticipated Transient Without Scram accidents were also found to be non-negligible contributors. The numerical results are largely driven by common mode failure probability estimates and to some extent, human error. Because of significant data and analysis uncertainties in these two areas (important, for instance, to the most dominant scenario in this study), it is recommended that the results of the uncertainty and sensitivity analyses be considered before any actions are taken based on this analysis

Predicting stem borer density in maize using RapidEye data and generalized linear models

Science.gov (United States)

Abdel-Rahman, Elfatih M.; Landmann, Tobias; Kyalo, Richard; Ong'amo, George; Mwalusepo, Sizah; Sulieman, Saad; Ru, Bruno Le

2017-05-01

Average maize yield in eastern Africa is 2.03 t ha-1 as compared to global average of 6.06 t ha-1 due to biotic and abiotic constraints. Amongst the biotic production constraints in Africa, stem borers are the most injurious. In eastern Africa, maize yield losses due to stem borers are currently estimated between 12% and 21% of the total production. The objective of the present study was to explore the possibility of RapidEye spectral data to assess stem borer larva densities in maize fields in two study sites in Kenya. RapidEye images were acquired for the Bomet (western Kenya) test site on the 9th of December 2014 and on 27th of January 2015, and for Machakos (eastern Kenya) a RapidEye image was acquired on the 3rd of January 2015. Five RapidEye spectral bands as well as 30 spectral vegetation indices (SVIs) were utilized to predict per field maize stem borer larva densities using generalized linear models (GLMs), assuming Poisson ('Po') and negative binomial ('NB') distributions. Root mean square error (RMSE) and ratio prediction to deviation (RPD) statistics were used to assess the models performance using a leave-one-out cross-validation approach. The Zero-inflated NB ('ZINB') models outperformed the 'NB' models and stem borer larva densities could only be predicted during the mid growing season in December and early January in both study sites, respectively (RMSE = 0.69-1.06 and RPD = 8.25-19.57). Overall, all models performed similar when all the 30 SVIs (non-nested) and only the significant (nested) SVIs were used. The models developed could improve decision making regarding controlling maize stem borers within integrated pest management (IPM) interventions.

Flight potential of the emerald ash borer

Science.gov (United States)

Leah S. Bauer; Deborah L. Miller; Robin A.J. Taylor; Robert A. Haack

2004-01-01

The emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is an invasive pest of ash trees (Fraxinus spp.) in North America. Native to several Asian countries, EAB was discovered in six southeastern Michigan counties and southwestern Ontario in 2002. EAB presumably emerged from infested solid wood...

Role of Melatonin in Cell-Wall Disassembly and Chilling Tolerance in Cold-Stored Peach Fruit.

Science.gov (United States)

Cao, Shifeng; Bian, Kun; Shi, Liyu; Chung, Hsiao-Hang; Chen, Wei; Yang, Zhenfeng

2018-06-06

Melatonin reportedly increases chilling tolerance in postharvest peach fruit during cold storage, but information on its effects on cell-wall disassembly in chilling-injured peaches is limited. In this study, we investigated the role of cell-wall depolymerization in chilling-tolerance induction in melatonin-treated peaches. Treatment with 100 μM melatonin alleviated chilling symptoms (mealiness) characterized by a decrease in fruit firmness and increase in juice extractability in treated peaches during storage. The loss of neutral sugars, such as arabinose and galactose, in both the 1,2-cyclohexylenedinitrilotetraacetic acid (CDTA)- and Na 2 CO 3 -soluble fractions was observed at 7 days in treated peaches, but the contents increased after 28 days of storage. Atomic-force-microscopy (AFM) analysis revealed that the polysaccharide widths in the CDTA- and Na 2 CO 3 -soluble fractions in the treated fruit were mainly distributed in a shorter range, as compared with those in the control fruit. In addition, the expression profiles of a series of cell-wall-related genes showed that melatonin treatment maintained the balance between transcripts of PpPME and PpPG, which accompany the up-regulation of several other genes involved in cell-wall disassembly. Taken together, our results suggested that the reduced mealiness by melatonin was probably associated with its positive regulation of numerous cell-wall-modifying enzymes and proteins; thus, the depolymerization of the cell-wall polysaccharides in the peaches treated with melatonin was maintained, and the treated fruit could soften gradually during cold storage.

New insights into the properties of pubescent surfaces: peach fruit as a model.

Science.gov (United States)

Fernández, Victoria; Khayet, Mohamed; Montero-Prado, Pablo; Heredia-Guerrero, José Alejandro; Liakopoulos, Georgios; Karabourniotis, George; Del Río, Víctor; Domínguez, Eva; Tacchini, Ignacio; Nerín, Cristina; Val, Jesús; Heredia, Antonio

2011-08-01

The surface of peach (Prunus persica 'Calrico') is covered by a dense indumentum, which may serve various protective purposes. With the aim of relating structure to function, the chemical composition, morphology, and hydrophobicity of the peach skin was assessed as a model for a pubescent plant surface. Distinct physicochemical features were observed for trichomes versus isolated cuticles. Peach cuticles were composed of 53% cutan, 27% waxes, 23% cutin, and 1% hydroxycinnamic acid derivatives (mainly ferulic and p-coumaric acids). Trichomes were covered by a thin cuticular layer containing 15% waxes and 19% cutin and were filled by polysaccharide material (63%) containing hydroxycinnamic acid derivatives and flavonoids. The surface free energy, polarity, and work of adhesion of intact and shaved peach surfaces were calculated from contact angle measurements of water, glycerol, and diiodomethane. The removal of the trichomes from the surface increased polarity from 3.8% (intact surface) to 23.6% and decreased the total surface free energy chiefly due to a decrease on its nonpolar component. The extraction of waxes and the removal of trichomes led to higher fruit dehydration rates. However, trichomes were found to have a higher water sorption capacity as compared with isolated cuticles. The results show that the peach surface is composed of two different materials that establish a polarity gradient: the trichome network, which has a higher surface free energy and a higher dispersive component, and the cuticle underneath, which has a lower surface free energy and higher surface polarity. The significance of the data concerning water-plant surface interactions is discussed within a physiological context.

Decolorization of textile industry wastewater in solid state fermentation with Peach-Palm (Bactris gasipaes residue

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J. A. Chicatto

2018-02-01

Full Text Available Abstract In this work we have assessed the decolorization of textile effluents throughout their treatment in a solid-state fermentation (SSF system. SSF assays were conducted with peach-palm (Bactris gasipaes residue using the white rot fungus Ganoderma lucidum EF 31. The influence of the dye concentration and of the amounts of peach-palm residue and liquid phase on both the discoloration efficiency and enzyme production was studied. According to our results, independently of experimental conditions employed, laccase was the main ligninolytic enzyme produced by G. lucidum. The highest laccase activity was obtained at very low effluent concentrations, suggesting the existence of an inhibitory effect of higher concentrations on fungal metabolism. The highest percentage of color removal was reached when 10 grams of peach palm residue was moistened with 60 mL of the final effluent. In control tests carried out with the synthetic dye Remazol Brilliant Blue R (RBBR decolorization efficiencies about 20% higher than that achieved with the industrial effluent were achieved. The adsorption of RBBR on peach-palm residue was also investigated. Equilibrium tests showed that the adsorption of this dye followed both Langmuir and Freundlich isotherms. Hence, our experimental results indicate that peach-palm residue is suitable substrate for both laccase production and color removal in industrial effluents.

Decolorization of textile industry wastewater in solid state fermentation with Peach-Palm (Bactris gasipaes) residue.

Science.gov (United States)

Chicatto, J A; Rainert, K T; Gonçalves, M J; Helm, C V; Altmajer-Vaz, D; Tavares, L B B

2018-02-15

In this work we have assessed the decolorization of textile effluents throughout their treatment in a solid-state fermentation (SSF) system. SSF assays were conducted with peach-palm (Bactris gasipaes) residue using the white rot fungus Ganoderma lucidum EF 31. The influence of the dye concentration and of the amounts of peach-palm residue and liquid phase on both the discoloration efficiency and enzyme production was studied. According to our results, independently of experimental conditions employed, laccase was the main ligninolytic enzyme produced by G. lucidum. The highest laccase activity was obtained at very low effluent concentrations, suggesting the existence of an inhibitory effect of higher concentrations on fungal metabolism. The highest percentage of color removal was reached when 10 grams of peach palm residue was moistened with 60 mL of the final effluent. In control tests carried out with the synthetic dye Remazol Brilliant Blue R (RBBR) decolorization efficiencies about 20% higher than that achieved with the industrial effluent were achieved. The adsorption of RBBR on peach-palm residue was also investigated. Equilibrium tests showed that the adsorption of this dye followed both Langmuir and Freundlich isotherms. Hence, our experimental results indicate that peach-palm residue is suitable substrate for both laccase production and color removal in industrial effluents.

Investigation of the aroma of commercial peach (Prunus persica L. Batsch) types by Proton Transfer Reaction-Mass Spectrometry (PTR-MS) and sensory analysis

NARCIS (Netherlands)

Cardoso Ferreira Pinhancos de Bianchi, Tiago; Weesepoel, Yannick; Koot, Alex; Iglesias, Ignasi; Eduardo, Iban; Gratacós-Cubarsí, Marta; Guerrero, Luis; Hortós, Maria; Ruth, van Saskia

2017-01-01

The aim of this study was to investigate the aroma and sensory profiles of various types of peaches (Prunus persica L. Batsch.). Forty-three commercial cultivars comprising peaches, flat peaches, nectarines, and canning peaches (pavías) were grown over two consecutive harvest years. Fruits were

Mild temperatures on bud breaking dormancy in peaches Temperaturas amenas na superação da dormência de pessegueiros

Directory of Open Access Journals (Sweden)

Geraldo Chavarria

2009-10-01

Full Text Available The search for low chill cultivars for peach production in areas with low chill accumulation (temperatures below 7.2°C has stimulated studies about the higher temperatures effects on low chill cultivars. The objective of this research was to evaluate the influence of mild temperatures on flowering and leafing of 'Turmalina' and 'Ágata' cultivars with low and medium low chilling requirements, respectively. Plants obtained by grafting flower twigs in the autumn, after the grafting, were submitted to chilling treatments (150, 250, 350, 500 and 672 hours at three temperature levels (5°C, 10°C and 15°C. After the end of the treatments, the plants had been taken to greenhouse (20°C±5°C and evaluated weekly. The results showed that satisfactory flower and leaf development occur even at temperatures as high as 15°C for a period of 150 hours, in 'Turmalina' peach. Temperatures of 10°C for a period equal or superior to 350 hours promote satisfactory leafing in 'Ágata' peach. Temperatures of 10°C and 15°C cause earlier development of flowers and leaves in both cultivars when compared to 5°C.A busca por cultivares pessegueiro com baixa necessidade em frio para regiões com pouco acúmulo de frio (temperaturas abaixo de 7,2°C tem estimulado estudos sobre o efeito de altas temperaturas sobre cultivares de baixa necessidade de frio. O objetivo desta pesquisa foi avaliar a influência de temperaturas amenas na floração e brotação da 'Turmalina' e 'Ágata', duas cultivares com baixa e média necessidade de frio, respectivamente. Plantas obtidas por enxertia no outono foram submetidas a tratamentos de frio (150, 250, 350, 500 e 672 horas em três níveis de temperatura (5°C, 10°C e 15°C. Após os tratamentos, estas plantas foram colocadas em casa de vegetação a temperatura de 20°C±5°C e avaliadas semanalmente. Conclui-se que temperatura de até 15°C por um período de 150 horas é capaz de resultar em floração e brotação satisfat

Susceptibility of peach GF 305 seedlings and selected herbaceous plants to plum pox virus isolates from western Slovakia.

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Glasa, M; Matisová, J; Hricovský, I; Kúdela, O

1997-12-01

The susceptibility of peach GF 305 seedlings and herbaceous plants to five plum pox virus (PPV) isolates from orchards of western Slovakia was investigated. PPV was isolated from diseased plum, apricot and peach trees, and transmitted by chip-budding to peach GF 305. The herbaceous plants were infected by mechanical inoculation. The transmission was analysed by symptomatology and double sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Infected peaches developed leaf distortion, tissue clearing along the veins and small chlorotic spots (isolate BOR-3). With exception of BOR-3, the PPV isolates transmitted from peach caused local chlorotic spots on Chenopodium foetidum. The character of symptoms changed when a sap from PPV-infected Nicotiana benthamiana was used as virus inoculum. From N. benthamiana, the PPV isolates could be transmitted to Pisum sativum, cv. Colmo (light green mosaic), N. clevelandii and N. clevelandii x N. glutinosa hybrid (latent infection or chlorotic spots).

Peach and apricot stone combustion in a bubbling fluidized bed

Energy Technology Data Exchange (ETDEWEB)

Kaynak, B.; Atimtay, Aysel T. [Department of Environmental Engineering, Middle East Technical University, Ankara 06531 (Turkey); Topal, H. [Department of Mechanical Engineering, Engineering and Architecture Faculty, Gazi University, Ankara 06570 (Turkey)

2005-07-25

In this study, a bubbling fluidized bed combustor (BFBC) of 102 mm inside diameter and 900 mm height was used to investigate the combustion characteristics of peach and apricot stones produced as a waste from the fruit juice industry. A lignite coal was also burned in the same combustor. The combustion characteristics of the wastes were compared with that of a lignite coal that is most widely used in Turkey. On-line concentrations of O{sub 2}, CO, CO{sub 2}, SO{sub 2}, NO{sub X} and total hydrocarbons (C{sub m}H{sub n}) were measured in the flue gas during combustion experiments. By changing the operating parameters (excess air ratio, fluidization velocity, and fuel feed rate), the variation of emissions of various pollutants was studied. Temperature distribution along the bed was measured with thermocouples. During the combustion tests, it was observed that the volatile matter from peach and apricot stones quickly volatilizes and mostly burn in the freeboard. The temperature profiles along the bed and the freeboard also confirmed this phenomenon. It was found that as the volatile matter of fruit stones increases, the combustion takes place more in the freeboard region. The results of this study have shown that the combustion efficiencies ranged between 98.8% and 99.1% for coal, 96.0% and 97.5% for peach stone and 93.4% and 96.3% for apricot stones. The coal has zero CO emission, but biomass fuels have very high CO emission which indicates that a secondary air addition is required for the system. SO{sub 2} emission of the coal is around 2400-2800 mg/Nm{sup 3}, whereas the biomass fuels have zero SO{sub 2} emission. NO{sub x} emissions are all below the limits set by the Turkish Air Quality Control Regulation of 1986 (TAQCR) for all tests. As the results of combustion of two biomass fuels are compared with each other, peach stones gave lower CO and NO{sub x} emissions but the SO{sub 2} emissions are a little higher than for apricot stones. These results suggest that

Modified atmosphere packaging extending the storage life of 'douradão' peach

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Ligia Regina Radomille de Santana

2010-12-01

Full Text Available 'Douradão' peach is a perishable product and when cold stored is subject to chilling injury. The objective of the experiment was to evaluate the effect of modified atmosphere packaging (MAP and cold storage on quality and storage life of these peaches. Fruits were packed in polypropylene (PP trays and placed inside low density polyethylene (LDPE bags (30, 50, 60, 75 μm thickness with active modified atmosphere (10 kPa CO2 + 1.5kPa O2, balance N2. The control was made with peaches held in nonwrapped PP trays. Fruits were kept at 1 ± 1 °C and 90 ± 5% relative humidity (RH for 28 days and CO2 and O2 within packages was monitored every two days. After 14, 21 and 28 days, samples were withdrawn from MAP and kept in air at 25 ± 1 °C and 90 ± 5% RH for ripening. On the day of removal from the cold storage and after 4 days, peaches were evaluated for weight loss, decay incidence, flesh firmness, woolliness incidence, soluble solids content (SSC, titratable acidity (TA and juice content. The results showed that MAP had influence on reducing weight loss and prevented postharvest decay. MAP of 1-2 kPa O2 and 3-6 kPa CO2 at 1 °C (from 50 and 60 μm LDPE films were effective for keeping good quality of 'Douradão' peaches during 28 days of storage, the ripe fruits showed reduced incidence of woolliness, adequate juiciness and flesh firmness. Packages of 30 and 75 μm LDPE films were ineffective for reducing woolliness during cold storage. MAP fruits showed lower SSC and no relevant effect on TA. Control fruits did not present marketable conditions after 14 days of cold storage.

Characterization of Ant Communities (Hymenoptera: Formicidae in Twigs in the Leaf Litter of the Atlantic Rainforest and Eucalyptus Trees in the Southeast Region of Brazil

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Debora R. de Souza

2012-01-01

Full Text Available Fragments of Atlantic Rainforest and extensive eucalyptus plantations are part of the landscape in the southeast region of Brazil. Many studies have been conducted on litter ant diversity in these forests, but there are few reports on the nesting sites. In the present study, we characterized the ant communities that nest in twigs in the leaf litter of dense ombrophilous forests and eucalyptus trees. The colony demographics associated with the physical structure of the nest were recorded. In the eucalyptus forests, the study examined both managed and unmanaged plantations. During five months, all undecomposed twigs between 10 and 30 cm in length containing ants found within a 16-m2 area on the surface of the leaf litter were collected. A total of 307 nests and 44 species were recorded. Pheidole, Solenopsis, and Camponotus were the most represented genera. Pheidole sp.13, Pheidole sp.43 and Linepithema neotropicum were the most populous species. The dense ombrophilous forest and a eucalyptus plantation unmanaged contained the highest number of colonized twigs; these communities were the most similar and the most species rich. Our results indicate that the twigs are important resources as they help to maintain the litter diversity of dense rain forest and abandoned eucalypt crops.

Population genetics and biological control of goldspotted oak borer, an invasive pest of California oaks

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Vanessa Lopez; Paul F. Rugman-Jones; Tom W. Coleman; Richard Stouthamer; Mark Hoddle

2015-01-01

California’s oak woodlands are threatened by the recent introduction of goldspotted oak borer (Agrilus auroguttatus). This invasive wood-borer is indigenous to mountain ranges in southern Arizona where its low population densities may be due to the presence of co-evolved, host-specific natural enemies. Reuniting A. auroguttatus...

Prunus hybrids rootstocks for flat peach

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Pilar Legua

2012-02-01

Full Text Available Peach (Prunus persica L. is the most important stone fruit tree grown in Spain and is the second most important fruit crop in Europe. The influence of eight Prunus rootstocks (GF-677, Krymsk® 86, PADAC 97-36, PADAC 99-05, PADAC 9912-03, PADAC 0024-01, PAC 0021-01 and PAC 0022-01 on vigor, yield and fruit quality traits of 'UFO 3' flat peach cultivar was studied. The highest trunk cross sectional area was exhibited by GF-677 and the lowest by PADAC 99-05, while intermediate values were found on the other rootstocks. The highest yield efficiency was found on PADAC 99-05, PAC 0021-01, PAC 0022-01 and PADAC 0024-01 and the lowest was shown on Krymsk® 86. The fruit quality parameters measured were color, fruit and stone weights, equatorial diameter, pulp thickness, pulp yield, firmness, pH, soluble solids content and titratable acidity. 'UFO 3' grafted on GF-677 resulted in the largest fruit weight, while the smallest was on PADAC 99-05. Fruits of 'UFO 3' showed a tendency to have higher firmness, higher red colored skin and RI when grafted on PADAC 99-05.

Sensory evaluation of Regina freestone peaches treated with low doses of gamma radiation

International Nuclear Information System (INIS)

O'Mahony, M.; Wong, S.Y.; Odbert, N.

1985-01-01

Sensory appraisal of low post-harvest gamma irradiation dosing (65-75 Krad) of a single batch of peaches revealed significant differences in aroma and in taste components not associated with sweetness, but only slight differences in firmness and appearance. A panel of practiced judges evaluated irradiated and non-irradiated peaches using a technique of minimal cross-sensory inference. The significant differences in aroma and taste also were detected by untrained judges

CONTRIBUTIONS TO IMPROVING CULTURE TEHNOLOGIES OF PEACHES GROWN ON SANDY SOILS THE SOUTH OF OLTENIA

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Anica Durau

2013-12-01

Full Text Available Technological factors with major implications in obtaining high yields and quality in peaches grown on sandy soils are planting row distance and shape of the crown, soil maintenance system, chemical, organic and foliar fertilzation. A smal size combined with the flatening of the crowns of the tres alows a dense planting, also ensure proper mechanization of work and easy penetration of light to the leaves and fruits. Crown form vertical belt proved to be suitable for al planting distances studied, easily made and maintained, having fruit production ranged betwen 15.9 t / ha at a distance of 2 m, 10.3 t / ha at a distance of 2.5 m and 7.9 t / ha at a distance of 3 m. The state of soil nutrient supply influence sucesful peach crop on sandy soils. The fertilzer dose of technology to N10 P80 K10 kg s.a / ha production was 34.9 t / ha. Organic fertilzation also contributes to obtaining high yields of peach. In sandy soil conditions most fruit production of 9.6 t / ha was obtained by fertilzation with organic manure 60t/ha. Besides fertilzation, soil maintenance system is one important link in the technology peach crop on sandy soils. The results found that the biggest peach fruit production was obtained from field maintenance system black-8,2t/ha. Using technology in foliar peaches culture on sandy soils, is an important means of providing nutrients that lead to improved proceses of growth and fructification. The best way is with foliar fertilzation Folibor in dose 5l/ha, the production obtained was 12.4 t /ha.

Canopy structure and physiology related to rootstock vigour in early-ripening peach cultivar Flordastar

International Nuclear Information System (INIS)

Motisi, A.; Grutta, I.; Pernice, F.; Caruso, T.

2005-01-01

Canopy architectural and eco-physiological traits were measured on five-year-old early-ripening peach cv Flordastar trees grafted on GF 677 and MrS 2/5 rootstocks. Data are reported both on measurements performed directly on the trees, for branches and twigs characters, and on the fractal dimension (D), estimated by the 'box counting' method taken from digital images of Winter-dormant trees, adopted as an indicator of canopy complexity. Results are discussed in relation to the modification of the canopy microclimate as a consequence of the effects of rootstock on tree architecture and water consumption, the latter measured by using sap flow (HPV) probes. A lower degree of canopy complexity was observed in trees grafted onto MrS 2/5 and this, in turn, was related to a higher degree of aerodynamic contact of the tree with the atmosphere (expressed in terms of leaf boundary conductance) and to a higher solar radiation intensity along the canopy profile. These differences did not affect fruit quality in terms of size, red skin over-colour and soluble solid content. In MrS 2/5, the higher light availability at all levels along canopy profile was related to a moderate water deficit status, even under full-irrigation conditions, as evidenced by the lower stem water potential (below -1.3 MPa) and by a lower transpiration rate (about one-half of the values observed on GF 677). At tree-level, MrS 2/5 had a daily water consumption that, also in relation to the lower leaf area per tree, resulted as low as 25% of the values observed on GF 677. The latter, even carrying a significantly higher leaf area and higher water consumption, never showed apparent symptoms of water deficit [it

Consumption, preferences and habits of purchasing consumers of peaches and nectarines

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Gener Augusto Penso

2018-06-01

Full Text Available Abstract In Brazil, there are few studies and information available on consumer preferences for peaches and nectarines. This lack of information, has hindered some aspects of the production chain, as the choice and exploitation of more appropriate cultivars, offer periods, adoption of marketing and marketing strategies, consequently affecting the availability and commercialization of fruits. This study aimed to analyze the consumer preference of peaches and nectarines, evaluate the traits observed for their acquisition, and the marketplace where they are preferably purchased. The research was carried out from November 2016 to February 2017, using the descriptive research methodology, by Survey method and Web 2.0. A structured questionnaire was applied with closed questions. An online form questionnaire was prepared using the Google docs application and also distributed online, through the internet social Medias. 610 people participated in the survey, with predominance of consumers living in the South and Southeast regions of the country, in the age groups of 21 to 30 years old, with a predominance of female participants. Consumption is mainly in the in natura form, and often predominates certain times a year. Consumers buy these fruits mainly in the supermarkets/hypermarkets. The consumers of peaches and nectarines in Brazil have a higher preference for fruits with yellow flesh and red epidermis, followed by fruits with white flesh and intense red epidermis, both preferably with free stone. The main criteria for the acquisition of peaches and nectarines in Brazil are fruit appearance (presence and/or absence of defects, bruises, etc.; followed by price, color of epidermis and fruit size.

Chromatin-associated regulation of sorbitol synthesis in flower buds of peach.

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Lloret, Alba; Martínez-Fuentes, Amparo; Agustí, Manuel; Badenes, María Luisa; Ríos, Gabino

2017-11-01

PpeS6PDH gene is postulated to mediate sorbitol synthesis in flower buds of peach concomitantly with specific chromatin modifications. Perennial plants have evolved an adaptive mechanism involving protection of meristems within specialized structures named buds in order to survive low temperatures and water deprivation during winter. A seasonal period of dormancy further improves tolerance of buds to environmental stresses through specific mechanisms poorly known at the molecular level. We have shown that peach PpeS6PDH gene is down-regulated in flower buds after dormancy release, concomitantly with changes in the methylation level at specific lysine residues of histone H3 (H3K27 and H3K4) in the chromatin around the translation start site of the gene. PpeS6PDH encodes a NADPH-dependent sorbitol-6-phosphate dehydrogenase, the key enzyme for biosynthesis of sorbitol. Consistently, sorbitol accumulates in dormant buds showing higher PpeS6PDH expression. Moreover, PpeS6PDH gene expression is affected by cold and water deficit stress. Particularly, its expression is up-regulated by low temperature in buds and leaves, whereas desiccation treatment induces PpeS6PDH in buds and represses the gene in leaves. These data reveal the concurrent participation of chromatin modification mechanisms, transcriptional regulation of PpeS6PDH and sorbitol accumulation in flower buds of peach. In addition to its role as a major translocatable photosynthate in Rosaceae species, sorbitol is a widespread compatible solute and cryoprotectant, which suggests its participation in tolerance to environmental stresses in flower buds of peach.

Molecular characterization of peach [Prunus persica (L.) Batsch] germplasm in the United States using microsatellite markers

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Peach [Prunus persica (L.) Batsch] is an important medicinal fruit with immense health benefits and antioxidant activity. In this study, microsatellite markers were used as DNA fingerprinting tools for the identification and characterization of peach germplasm in the United States. Eleven microsatel...

Emerald ash borer biology and invasion history

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Robert A. Haack; Yuri Baranchikov; Leah S. Bauer; Therese M. Poland

2015-01-01

The emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is native to eastern Asia and is primarily a pest of ash (Fraxinus) trees (Fig. 1). Established populations of EAB were first detected in the United States and Canada in 2002 (Haack et al., 2002), and based on a dendrochronology study by Siegert...

Field host range testing of Spathius agrili, a parasitoid of emerald ash borer: evaluating nontarget impacts

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John S. Strazanac; Juli R. Gould; Robert A. Haack; Ivich Fraser

2008-01-01

The introduction of the emerald ash borer (EAB), Agrilis planipennis (Coleoptera: Buprestidae), into the Midwest from Asia has had a devastating affect on ash (Fraxinus spp.). As the emerald ash borer's ability to spread became better understood and its distribution in the Midwest increased, biocontrol became an increasingly...

Self-compatible peach (Prunus persica) has mutant versions of the S haplotypes found in self-incompatible Prunus species.

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Tao, Ryutaro; Watari, Akiko; Hanada, Toshio; Habu, Tsuyoshi; Yaegaki, Hideaki; Yamaguchi, Masami; Yamane, Hisayo

2007-01-01

This study demonstrates that self-compatible (SC) peach has mutant versions of S haplotypes that are present in self-incompatible (SI) Prunus species. All three peach S haplotypes, S (1), S (2), and S (2m), found in this study encode mutated pollen determinants, SFB, while only S (2m) has a mutation that affects the function of the pistil determinant S-RNase. A cysteine residue in the C5 domain of the S (2m)-RNase is substituted by a tyrosine residue, thereby reducing RNase stability. The peach SFB mutations are similar to the SFB mutations found in SC haplotypes of sweet cherry (P. avium) and Japanese apricot (P. mume). SFB (1) of the S (1) haplotype, a mutant version of almond (P. dulcis) S (k) haplotype, encodes truncated SFB due to a 155 bp insertion. SFB (2) of the S (2) and S (2m) haplotypes, both of which are mutant versions of the S (a) haplotype in Japanese plum (P. salicina), encodes a truncated SFB due to a 5 bp insertion. Thus, regardless of the functionality of the pistil determinant, all three peach S haplotypes are SC haplotypes. Our finding that peach has mutant versions of S haplotypes that function in almond and Japanese plum, which are phylogenetically close and remote species, respectively, to peach in the subfamily Prunoideae of the Roasaceae, provides insight into the SC/SI evolution in Prunus. We discuss the significance of SC pollen part mutation in peach with special reference to possible differences in the SI mechanisms between Prunus and Solanaceae.

Effects of different products of peach (Prunus persica L. Batsch) from a variety developed in southern Brazil on oxidative stress and inflammatory parameters in vitro and ex vivo

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Gasparotto, Juciano; Somensi, Nauana; Bortolin, Rafael Calixto; Moresco, Karla Suzana; Girardi, Carolina Saibro; Klafke, Karina; Rabelo, Thallita Kelly; Morrone, Maurilio Da Silva; Vizzotto, Márcia; Raseira, Maria do Carmo Bassols; Moreira, José Claudio Fonseca; Gelain, Daniel Pens

2014-01-01

Antioxidant, anti-glycation and anti-inflammatory activities of fresh and conserved peach fruits (Prunus persica L. Batsch) were compared. Fresh peach pulps, peels, preserve peach pulps and the preserve syrup were prepared at equal concentrations. Rat liver, kidney and brain cortex tissue slices were pre-incubated with peach samples, subjected to oxidative stress with FeSO4 and hydrogen peroxide. Fresh peach pulps and peel conferred higher protection against cytotoxicity and oxidative stress than preserve peach pulps in most tissues. Release of tumor necrosis factor-α and interleukin-1β was also significantly decreased by Fresh peach pulps and peel, followed by preserve peach pulps. Total phenolic determination and HPLC analysis of carotenoids showed that the content of secondary metabolites in Fresh peach pulps and peel is significantly higher than in preserve peach pulps, while the syrup had only small or trace amounts of these compounds. Fresh peach pulps and Peel demonstrated high antioxidant and anti-inflammatory effects preventing against induced damage. PMID:25320458

The overwintering physiology of the emerald ash borer, Agrilus planipennis fairmaire (coleoptera: buprestidae).

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Crosthwaite, Jill C; Sobek, Stephanie; Lyons, D Barry; Bernards, Mark A; Sinclair, Brent J

2011-01-01

Ability to survive cold is an important factor in determining northern range limits of insects. The emerald ash borer (Agrilus planipennis) is an invasive beetle introduced from Asia that is causing extensive damage to ash trees in North America, but little is known about its cold tolerance. Herein, the cold tolerance strategy and mechanisms involved in the cold tolerance of the emerald ash borer were investigated, and seasonal changes in these mechanisms monitored. The majority of emerald ash borers survive winter as freeze-intolerant prepupae. In winter, A. planipennis prepupae have low supercooling points (approximately -30°C), which they achieve by accumulating high concentrations of glycerol (approximately 4M) in their body fluids and by the synthesis of antifreeze agents. Cuticular waxes reduce inoculation from external ice. This is the first comprehensive study of seasonal changes in cold tolerance in a buprestid beetle. 2010 Elsevier Ltd. All rights reserved.

Transcriptomic and Functional Analyses Reveal That PpGLK1 Regulates Chloroplast Development in Peach (Prunus persica

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Min Chen

2018-01-01

Full Text Available Peach is an ideal species for fruit tree research because of its small, fully sequenced genome. Chloroplast development is dependent on the tight cooperation between the nuclear and plastid genomes, and is regulated by GLK transcription factors. In this work, the pigment content was monitored and the chloroplast-to-chromoplast conversion during the fruit ripening was visualized by transmission electron microscopy. Localization and expression analyses showed that PpGLK1 was located in the nucleus and expressed mainly in the leaves and fruit skin. A transcriptome analysis showed that PpGLK1 and its target genes were significantly differentially expressed in ripening peach fruit skin. PpGLK1 silencing affected chlorophyll accumulation in peach leaves and fruits. Overexpression of PpGLK1 rescued the phenotypes of the Arabidopsis Atglk1Atglk2 double mutant and the tomato uniform ripening mutant. The results of a yeast two-hybrid analysis showed that PpGLK1 is autoactivated and that PpGLK1 (301-542 a.a. interacted with PpARF5. Together, our results indicate that PpGLK1 regulates chloroplast development in green tissues in peach. Therefore, it may be a promising target gene for improving the production and quality of peach by genetic engineering and breeding approaches.

A coffee berry borer (Coleoptera: Curculionidae: Scolytinae) bibliography

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One hundred years ago, one of the most significant biological invasions of an agricultural insect pest in the Americas was initiated. Endemic to Africa, the coffee berry borer (Hypothenemus hampei; Coleoptera: Curculionidae) was accidentally introduced to Brazil in 1913 and years later invaded coffe...

Production and evaluation of mineral and nutrient contents, chemical composition, and sensory properties of ice creams fortified with laboratory-prepared peach fibre

Science.gov (United States)

Yangılar, Filiz

2016-01-01

Background In the coming years, a nutraceutical food may provide both physical and mental benefits that are commonly attributed to the active components of the food. Objective In this study, we determined the nutrient and mineral contents, sensory properties, and physical and chemical characteristics of ice creams manufactured using peach fibre at different concentrations (1 and 2%). Method A total of five experimental groups were formed: two types (from peach peel and pulp) of flour, two fibre concentrations (1 and 2%), and a control group without fibres. Results Flour obtained from peach pulp and peel was found to have a significant (pice cream samples, especially the rates of Ca, K, Mg, and P, which increased in the samples depending on the content of peach fibre. Sensory ratings and acceptability of ice creams decreased significantly with increasing peach peel fibre, whereas ice creams made with C (control) and B1 (ice creams made from 1% peach pulp fibre) was the highest scored by the panellists. Conclusions Peach fibre concentrates might be used as a good source of nutraceutical ingredients. PMID:27814781

Agronomical Parameters, Sugar Profile and Antioxidant Compounds of “Catherine” Peach Cultivar Influenced by Different Plum Rootstocks

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Carolina Font i Forcada

2014-02-01

Full Text Available The influence of seven plum rootstocks (Adesoto, Monpol, Montizo, Puebla de Soto 67 AD, PM 105 AD, St. Julien GF 655/2 and Constantí 1 on individual and total sugars, as well as on antioxidant content in fruit flesh of “Catherine” peaches, was evaluated for three years. Agronomical and basic fruit quality parameters were also determined. At twelve years after budding, significant differences were found between rootstocks for the different agronomic and fruit quality traits evaluated. The Pollizo plum rootstocks Adesoto and PM 105 AD seem to induce higher sweetness to peach fruits, based on soluble solids content, individual (sucrose, fructose and sorbitol and total sugars. A clear tendency was also observed with the rootstock Adesoto, inducing the highest content of phenolics, flavonoids, vitamin C and relative antioxidant capacity (RAC. Thus, the results of this study demonstrate the significant effect of rootstock on the sugar profile and phytochemical characteristics of peach fruits. In addition, this work shows the importance of the sugar profile, because specific sugars play an important role in peach flavour quality, as well as the studied phytochemical compounds when looking for high quality peaches with enhanced health properties.

Genome-wide analysis of the homeodomain-leucine zipper (HD-ZIP) gene family in peach (Prunus persica).

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Zhang, C H; Ma, R J; Shen, Z J; Sun, X; Korir, N K; Yu, M L

2014-04-08

In this study, 33 homeodomain-leucine zipper (HD-ZIP) genes were identified in peach using the HD-ZIP amino acid sequences of Arabidopsis thaliana as a probe. Based on the phylogenetic analysis and the individual gene or protein characteristics, the HD-ZIP gene family in peach can be classified into 4 subfamilies, HD-ZIP I, II, III, and IV, containing 14, 7, 4, and 8 members, respectively. The most closely related peach HD-ZIP members within the same subfamilies shared very similar gene structure in terms of either intron/exon numbers or lengths. Almost all members of the same subfamily shared common motif compositions, thereby implying that the HD-ZIP proteins within the same subfamily may have functional similarity. The 33 peach HD-ZIP genes were distributed across scaffolds 1 to 7. Although the primary structure varied among HD-ZIP family proteins, their tertiary structures were similar. The results from this study will be useful in selecting candidate genes from specific subfamilies for functional analysis.

Interspecific proteomic comparisons reveal ash phloem genes potentially involved in constitutive resistance to the emerald ash borer.

Science.gov (United States)

Whitehill, Justin G A; Popova-Butler, Alexandra; Green-Church, Kari B; Koch, Jennifer L; Herms, Daniel A; Bonello, Pierluigi

2011-01-01

The emerald ash borer (Agrilus planipennis) is an invasive wood-boring beetle that has killed millions of ash trees since its accidental introduction to North America. All North American ash species (Fraxinus spp.) that emerald ash borer has encountered so far are susceptible, while an Asian species, Manchurian ash (F. mandshurica), which shares an evolutionary history with emerald ash borer, is resistant. Phylogenetic evidence places North American black ash (F. nigra) and Manchurian ash in the same clade and section, yet black ash is highly susceptible to the emerald ash borer. This contrast provides an opportunity to compare the genetic traits of the two species and identify those with a potential role in defense/resistance. We used Difference Gel Electrophoresis (DIGE) to compare the phloem proteomes of resistant Manchurian to susceptible black, green, and white ash. Differentially expressed proteins associated with the resistant Manchurian ash when compared to the susceptible ash species were identified using nano-LC-MS/MS and putative identities assigned. Proteomic differences were strongly associated with the phylogenetic relationships among the four species. Proteins identified in Manchurian ash potentially associated with its resistance to emerald ash borer include a PR-10 protein, an aspartic protease, a phenylcoumaran benzylic ether reductase (PCBER), and a thylakoid-bound ascorbate peroxidase. Discovery of resistance-related proteins in Asian species will inform approaches in which resistance genes can be introgressed into North American ash species. The generation of resistant North American ash genotypes can be used in forest ecosystem restoration and urban plantings following the wake of the emerald ash borer invasion.

Red oak borers become sterile when reared under continuous light

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Jimmy R. Galford

1975-01-01

Red oak borers, Enaphalodes rufulus (Haldeman), reared under continuous light for 12 weeks became sterile. Sterility is thought to have been caused by light destroying vitamins essential for fertility

Evaluation of stem borer resistance management strategies for Bt ...

African Journals Online (AJOL)

GREGORY

2011-06-01

Jun 1, 2011 ... Forages, sorghum and maize varieties were evaluated for stem borer preference and survivorship in the .... ecologies in Kenya to estimate the potential of natural refugia ..... teria such as fodder biomass, stem size, colour of.

Differential metabolic rearrangements after cold storage are correlated with chilling injury resistance of peach fruits

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Claudia A Bustamante

2016-09-01

Full Text Available Reconfiguration of the metabolome is a key component involved in the acclimation to cold in plants; however, few studies have been devoted to the analysis of the overall metabolite changes after cold storage of fruits prior to consumption. Here, metabolite profiling of six peach varieties with differential susceptibility to develop mealiness, a chilling-injury (CI symptom, was performed. According to metabolic content at harvest; after cold treatment; and after ripening, either following cold treatment or not; peach fruits clustered in distinct groups, depending on harvest-time, cold treatment, and ripening state. Both common and distinct metabolic responses among the six varieties were found; common changes including dramatic galactinol and raffinose rise; GABA, Asp and Phe increase; and 2-oxo-glutarate and succinate decrease. Raffinose content after long cold treatment quantitatively correlated to the degree of mealiness resistance of the different peach varieties; and thus, raffinose emerges as a candidate biomarker of this CI disorder. Xylose increase after cold treatment was found only in the susceptible genotypes, indicating a particular cell wall reconfiguration of these varieties while being cold-stored. Overall, results indicate that peach fruit differential metabolic rearrangements due to cold treatment, rather than differential metabolic priming before cold, are better related with CI resistance. The plasticity of peach fruit metabolism renders it possible to induce a diverse metabolite array after cold, which is successful, in some genotypes, to avoid CI

Final summary report on the Peach Bottom End-of-Life Program

International Nuclear Information System (INIS)

Steward, K.P.

1978-07-01

HTGR design methods verifications have been performed under the Peach Bottom End-of-Life Program by comparison of actual with predicted physics, thermal, fission product, and materials behavior in Peach Bottom. These design methods verifications have utilized the data determined from nondestructive fuel and circuit gamma scanning on-site, from laboratory examinations of samples removed from the primary circuit, and from a complementary program of Peach Bottom fuel element postirradiation examinations at Oak Ridge National Laboratory. Fifty-five driver fuel elements were axially gamma scanned to determine fission product distributions for use in burnup calculations, power profile determinations, and fission product release and redistribution studies. The cesium plateout distribution in the primary circuit was subsequently mapped by gamma scanning the ducting at 12 locations, axially traversing 79 steam generator tubes with Cd Te detectors from the water side, and internally scanning two vertical runs of ducting. Component removal involved trepanning of the primary circuit ducting, obtaining access to the steam generator internals, and removing over 100 superheater, evaporator, and economizer tubing samples. During this phase, macroscopic examinations of the steam generator and ducting internals were performed. Subsequent laboratory examinations of removed samples included radiochemical tests to substantiate in-situ gamma scans and to determine 90 Sr distributions; metallurgical tests to evaluate surface films, microstructural changes, and residual mechanical properties; and tritium permeation tests to provide data for improving HTGR tritium release predictions. The condition and metallurgical integrity of all components examined were found to be excellent, with minimal coolant/substrate interaction and entirely acceptable residual mechanical properties. The suitability of the materials used in the construction of the Peach Bottom HTGR was thereby confirmed

Liquid chromatography/mass spectrometry investigation of the impact of thermal processing and storage on peach procyanidins.

Science.gov (United States)

Hong, Yun-Jeong; Barrett, Diane M; Mitchell, Alyson E

2004-04-21

Normal-phase liquid chromatography/mass spectrometry (LC/MS) was used to determine the levels and fate of procyanidins in frozen and canned Ross clingstone peaches as well as in the syrup used in the canning over a 3 month period. Procyanidin oligomers, monomers through undecamers, were identified in Ross clingstone peaches. Optimized methods allowed for the quantitation of oligomers through octamers. The profile of procyanidins in peaches is similar to profiles found in grapes, chocolate, and beverages linked to health benefits such as tea and wine. The monomer content in frozen peeled peaches was found to be 19.59 mg/kg. Dimers (39.59 mg/kg) and trimers (38.81 mg/kg) constituted the largest percent composition of oligomers in the peaches. Tetramers through octamers were present in levels of 17.81, 12.43, 10.62, 3.94 and 1.75 mg/kg, respectively. Thermal processing resulted in an 11% reduction in monomers, a 9% reduction in dimers, a 12% reduction in trimers, a 6% reduction in tetramers, and a 5% reduction in pentamers. Hexamers and heptamers demonstrated an approximate 30% loss, and octamers were no longer detected. Analysis of the syrup after thermal processing indicates that there is a migration of procyanidin monomers through hexamers into the syrup that can account for the losses observed during the canning process. Storage of canned peaches for 3 months demonstrated a time-related loss in higher oligomers and that by 3 months oligomers larger than tetramers are not observed. At 3 months postcanning, levels of monomers had decreased by 10%, dimers by 16%, trimers by 45%, and tetramers by 80%. A similar trend was observed in the canning syrup.

Ecological adaptation of wild peach palm, its in situ conservation and deforestation-mediated extinction in southern Brazilian Amazonia.

Science.gov (United States)

Clement, Charles R; Santos, Ronaldo P; Desmouliere, Sylvain J M; Ferreira, Evandro J L; Neto, João Tomé Farias

2009-01-01

The Arc of Fire across southern Amazonia seasonally attracts worldwide attention as forests are cut and burned for agricultural expansion. These forests contain numerous wild relatives of native South American crops, such as peach palm. Our prospecting expeditions examined critical areas for wild peach palm in the Arc of Fire in Mato Grosso, Pará, Maranhão and Tocantins, as well as areas not previously examined in Amazonas and Amapá states. Recent digitization of the RADAM Brasil project permitted comparison among RADAM's parataxonomists' observations, previous botanical collections and our prospecting. Mapping on soils and vegetation types enabled us to hypothesize a set of ecological preferences. Wild peach palm is best adapted to Ultisols (Acrisols) in open forests across the Arc of Fire and westward into the more humid western Amazonia. Populations are generally small (fewer than 10 plants) on slopes above watercourses. In northern Mato Grosso and southern Pará soybean fields and pastures now occupy numerous areas where RADAM identified wild peach palm. The controversial BR-163 Highway is already eroding wild peach palm as deforestation expands. Many of these populations are now isolated by increasing forest fragmentation, which will lead to decreased reproduction via inbreeding depression and eventual extinction even without complete deforestation. Federal conservation areas are less numerous in the Arc of Fire than in other parts of Brazilian Amazonia, although there are indigenous lands; these conservation areas contain viable populations of wild peach palm and require better protection than they are currently receiving. Ex situ conservation of these populations is not viable given the relative lack of importance of domesticated peach palm and the difficulty of maintaining even economically interesting genetic resources.

Apple and peach consumption habits across European countries

NARCIS (Netherlands)

Konopacka, D.; Jesionkowska, K.; Kruczynska, D.; Schoorl, F.W.

2010-01-01

The aim of this study was to gain information concerning apple and peach consumption frequency within different European countries in relation to age and gender. The survey was a part of a complex experiment with the aim of evaluating consumers’ preferences towards new varieties, and the data is

Progress on biological control of emerald ash borer

Science.gov (United States)

Leah S. Bauer; Houping Liu; Juli Gould

2008-01-01

The emerald ash borer (EAB), Agrilus planipennis, a buprestid native to northeastern Asia, was determined as the cause of ash tree (Fraxinus spp.) mortality in areas of southern Michigan and Ontario, Canada, in 2002. Infestations have been found since in Ohio, Indiana, Maryland, Virginia, Illinois, Pennsylvania, and West Virginia....

Performance and characteristics of carcass and non-carcass components of lambs fed peach-palm by-product.

Science.gov (United States)

dos Santos Cabral, Ícaro; Azevêdo, José Augusto Gomes; de Almeida, Flávio Moreira; Pereira, Luiz Gustavo Ribeiro; de Araújo, Gherman Garcia Leal; dos Santos Cruz, Cristiane Leal; Nogueira, Abdon Santos; Souza, Lígia Lins; de Oliveira, Gisele Andrade

2013-11-01

The objective of this study was to evaluate the effects of supplying the by-product of peach-palm (Bactris gasipaes) on performance and characteristics of carcass and non-carcass components of feedlot lambs. Twenty Santa Ines lambs of 150 days average age and 22.4 ± 3.4 kg body weight were confined in individual pens. A completely randomized design was utilized with four experimental diets composed of: fresh peach-palm by-product enriched with urea, fresh peach-palm by-product + concentrate, silage of peach-palm by-product + concentrate, and silage of peach-palm by-product enriched with 15 % corn meal + concentrate. Intake was evaluated daily, and at the end of 42 days of experiments, lambs were slaughtered and the characteristics of carcass and non-carcass parts were evaluated. Performance and carcass characteristics showed differences between the animals' intake of total mixed rations (TMR) and only the diet with roughage. For the lambs that intaked TMR, the form of utilization of roughage (fresh or as silage) affected animal performance but did not change the carcass characteristics. Dry matter intake and feed conversion were influenced by the form of utilization of the silage (with and without additive). Providing fresh by-product plus concentrate improves lamb performance but does not interfere in the carcass characteristics, compared with the use of by-product in the form of silage.

De novo transcriptome assembly of two different peach cultivars grown in Korea

Directory of Open Access Journals (Sweden)

Yeonhwa Jo

2015-12-01

Full Text Available Peach (Prunus persica is one of the most popular stone fruits worldwide. Next generation sequencing (NGS has facilitated genome and transcriptome analyses of several stone fruit trees. In this study, we conducted de novo transcriptome analyses of two peach cultivars grown in Korea. Leaves of two cultivars, referred to as Jangtaek and Mibaek, were harvested and used for library preparation. The two prepared libraries were paired-end sequenced by the HiSeq2000 system. We obtained 8.14 GB and 9.62 GB sequence data from Jangtaek and Mibaek (NCBI accession numbers: SRS1056585 and SRS1056587, respectively. The Trinity program was used to assemble two transcriptomes de novo, resulting in 110,477 (Jangtaek and 136,196 (Mibaek transcripts. TransDecoder identified possible coding regions in assembled transcripts. The identified proteins were subjected to BLASTP search against NCBI's non-redundant database for functional annotation. This study provides transcriptome data for two peach cultivars, which might be useful for genetic marker development and comparative transcriptome analyses.

Investigation of the aroma of commercial peach (Prunus persica L. Batsch) types by Proton Transfer Reaction-Mass Spectrometry (PTR-MS) and sensory analysis.

Science.gov (United States)

Bianchi, Tiago; Weesepoel, Yannick; Koot, Alex; Iglesias, Ignasi; Eduardo, Iban; Gratacós-Cubarsí, Marta; Guerrero, Luis; Hortós, Maria; van Ruth, Saskia

2017-09-01

The aim of this study was to investigate the aroma and sensory profiles of various types of peaches (Prunus persica L. Batsch.). Forty-three commercial cultivars comprising peaches, flat peaches, nectarines, and canning peaches (pavías) were grown over two consecutive harvest years. Fruits were assessed for chemical aroma and sensory profiles. Chemical aroma profile was obtained by proton transfer reaction-mass spectrometry (PTR-MS) and spectral masses were tentatively identified with PTR-Time of Flight-MS (PTR-Tof-MS). Sensory analysis was performed at commercial maturity considering seven aroma/flavor attributes. The four types of peaches showed both distinct chemical aroma and sensory profiles. Flat peaches and canning peaches showed most distinct patterns according to discriminant analysis. The sensory data were related to the volatile compounds by partial least square regression. γ-Hexalactone, γ-octalactone, hotrienol, acetic acid and ethyl acetate correlated positively, and benzeneacetaldehyde, trimethylbenzene and acetaldehyde negatively to the intensities of aroma and ripe fruit sensory scores. Copyright © 2017 Elsevier Ltd. All rights reserved.

Correlation between agronomic and stem borer resistant traits in ...

African Journals Online (AJOL)

GY had significant relationship with days to 50% pollen shed, (DTA) (rg = 0.49**), plant height (PH) (rg ... that either days to 50% pollen shed or plant height could be considered in selection for stem borer resistance. ... HOW TO USE AJOL.

Postharvest biological control of brown rot in peaches after cold storage preceded by preharvest chemical control 1

Directory of Open Access Journals (Sweden)

Elizandra Pivotto Pavanello

2015-12-01

Full Text Available ABSTRACT Pathogenic fungi cause skin darkening and peach quality depreciation in post harvest. Therefore, alternative techniques to chemical treatment are necessary in order to reduce risks to human health. The aim of this study was to evaluate the effect of the application of Trichoderma harzianum in association with different fungicides applied before harvest to 'Eldorado' peaches for brown rot control and other quality parameters during storage. The treatments consisted of five preharvest fungicide applications (control, captan, iprodione, iminoctadine and tebuconazole associated with postharvest application of T. harzianum, after cold storage (with and without application, in three evaluation times (zero, two and four days at 20 °C, resulting in a 5x2x3 factorial design. The application of T. harzianum only brought benefits to the control of brown rot when combined with the fungicide captan, at zero day shelf life. After two days, there was a greater skin darkening in peaches treated with T. harzianum compared with peaches without the treatment, except for peaches treated with the fungicide iprodione and T. harzianum The application of T. harzianum during postharvest showed no benefits for the control of brown rot, however, the association with fungicides reduced the incidence of Rhizopus stolonifer during the shelf life.

The relationship between trees and human health: evidence from the spread of the emerald ash borer.

Science.gov (United States)

Donovan, Geoffrey H; Butry, David T; Michael, Yvonne L; Prestemon, Jeffrey P; Liebhold, Andrew M; Gatziolis, Demetrios; Mao, Megan Y

2013-02-01

Several recent studies have identified a relationship between the natural environment and improved health outcomes. However, for practical reasons, most have been observational, cross-sectional studies. A natural experiment, which provides stronger evidence of causality, was used to test whether a major change to the natural environment-the loss of 100 million trees to the emerald ash borer, an invasive forest pest-has influenced mortality related to cardiovascular and lower-respiratory diseases. Two fixed-effects regression models were used to estimate the relationship between emerald ash borer presence and county-level mortality from 1990 to 2007 in 15 U.S. states, while controlling for a wide range of demographic covariates. Data were collected from 1990 to 2007, and the analyses were conducted in 2011 and 2012. There was an increase in mortality related to cardiovascular and lower-respiratory-tract illness in counties infested with the emerald ash borer. The magnitude of this effect was greater as infestation progressed and in counties with above-average median household income. Across the 15 states in the study area, the borer was associated with an additional 6113 deaths related to illness of the lower respiratory system, and 15,080 cardiovascular-related deaths. Results suggest that loss of trees to the emerald ash borer increased mortality related to cardiovascular and lower-respiratory-tract illness. This finding adds to the growing evidence that the natural environment provides major public health benefits. Published by Elsevier Inc.

Ecological adaptation of wild peach palm, its in situ conservation and deforestation-mediated extinction in southern Brazilian Amazonia.

Directory of Open Access Journals (Sweden)

Charles R Clement

Full Text Available BACKGROUND: The Arc of Fire across southern Amazonia seasonally attracts worldwide attention as forests are cut and burned for agricultural expansion. These forests contain numerous wild relatives of native South American crops, such as peach palm. METHODOLOGY/PRINCIPAL FINDINGS: Our prospecting expeditions examined critical areas for wild peach palm in the Arc of Fire in Mato Grosso, Pará, Maranhão and Tocantins, as well as areas not previously examined in Amazonas and Amapá states. Recent digitization of the RADAM Brasil project permitted comparison among RADAM's parataxonomists' observations, previous botanical collections and our prospecting. Mapping on soils and vegetation types enabled us to hypothesize a set of ecological preferences. Wild peach palm is best adapted to Ultisols (Acrisols in open forests across the Arc of Fire and westward into the more humid western Amazonia. Populations are generally small (fewer than 10 plants on slopes above watercourses. In northern Mato Grosso and southern Pará soybean fields and pastures now occupy numerous areas where RADAM identified wild peach palm. The controversial BR-163 Highway is already eroding wild peach palm as deforestation expands. CONCLUSIONS/SIGNIFICANCE: Many of these populations are now isolated by increasing forest fragmentation, which will lead to decreased reproduction via inbreeding depression and eventual extinction even without complete deforestation. Federal conservation areas are less numerous in the Arc of Fire than in other parts of Brazilian Amazonia, although there are indigenous lands; these conservation areas contain viable populations of wild peach palm and require better protection than they are currently receiving. Ex situ conservation of these populations is not viable given the relative lack of importance of domesticated peach palm and the difficulty of maintaining even economically interesting genetic resources.

Emerald ash borer dispersal in Maryland: go forth young pest

Science.gov (United States)

Chris Sargent; Dick Bean; Michael Raupp; Alan J. Sawyer

2009-01-01

The emerald ash borer (EAB) (Agrilus planipennis Fairmaire), an exotic invasive pest from Asia, was introduced into Maryland in April 2003 via infested nursery stock shipped from Michigan to a nursery in southern...

Emerald ash borer infestation rates in Michigan, Ohio, and Indiana.

Science.gov (United States)

Eric L. Smith; Andrew J. Storer; Bryan K. Roosien

2009-01-01

The goal of this study was to obtain an estimate of the infestation rate of ash trees with emerald ash borer (EAB) (Agrilus planipennis, Fairmaire; Coleoptera; Buprestidae), across its primary infestation zone of...

Role of emerald ash borer (Coleoptera: Buprestidae) larval vibrations in host-quality assessment by Tetrastichus planipennisi (Hymenoptera: Eulophidae).

Science.gov (United States)

Ulyshen, Michael D; Mankin, Richard W; Chen, Yigen; Duan, Jian J; Poland, Therese M; Bauer, Leah S

2011-02-01

The biological control agent Tetrastichus planipennisi Yang (Hymenoptera: Eulophidae) is a gregarious larval endoparasitoid of the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), an invasive cambium-feeding species responsible for recent, widespread mortality of ash (Fraxinus spp.) in North America. T. planipennisi is known to prefer late-instar emerald ash borer, but the cues used to assess host size by this species and most other parasitoids of concealed hosts remain unknown. We sought to test whether vibrations produced by feeding emerald ash borer vary with larval size and whether there are any correlations between these cues and T. planipennisi progeny number (i.e., brood size) and sex ratio. The amplitudes and rates of 3-30-ms vibrational impulses produced by emerald ash borer larvae of various sizes were measured in the laboratory before presenting the larvae to T. planipennisi. Impulse-rate did not vary with emerald ash borer size, but vibration amplitude was significantly higher for large larvae than for small larvae. T. planipennisi produced a significantly higher proportion of female offspring from large hosts than small hosts and was shown in previous work to produce more offspring overall from large hosts. There were no significant correlations, however, between the T. planipennisi progeny data and the emerald ash borer sound data. Because vibration amplitude varied significantly with host size, however, we are unable to entirely reject the hypothesis that T. planipennisi and possibly other parasitoids of concealed hosts use vibrational cues to assess host quality, particularly given the low explanatory potential of other external cues. Internal chemical cues also may be important.

Characterization of Amazon fibers of the peach palm, balsa, and babassu by XDR, TGA and NMR

International Nuclear Information System (INIS)

Martins, Maria A.; Marconcini, Jose M.; Morelli, Carolina L.; Marinelli, Alessandra L.; Bretas, Rosario E.S.

2011-01-01

The aim of this work was to present the results by testing X-ray diffraction (XRD), thermogravimetric analysis (TG), nuclear magnetic resonance (NMR) and determining the moisture content of the peach palm, balsa and babassu fibers for assessing the feasibility of composite materials. The fibers of peach palm, balsa and babassu showed characteristic chemical structure of lignocellulosic material, and good thermal stability up to 220 deg C. The fiber with the highest crystallinity index (Ic) is the peach palm (72%) and the less crystalline is the babassu (37%), while the balsa fibers have Ic equal to 64%. The results have shown that these fibers can be used in the manufacture of composite materials. (author)

Monitoring the establishment and abundance of introduced parasitoids of emerald ash borer larvae in Maryland, U.S.A

Science.gov (United States)

Classical biological control can be an important tool for managing invasive species such as emerald ash borer, Agrilus planipennis Fairmaire. Emerald ash borer is now widespread throughout the United States, and was first detected in Maryland in 2003. The biological control program to manage emera...

The effect of ultraviolet irradiation on shelf-life and ripening of peaches and apples

International Nuclear Information System (INIS)

Lu, J.Y.; Stevens, C.; Khan, V.A.; Kabwe, M.; Wilson, C.L.

1991-01-01

''Loring'' and ''Elberta'' peaches and ''Golden Delicious'' apples were irradiated with UV (254nm) to doses of 0.84 X 10(4) to 40 X 10(4) erg/mm2 then stored. ''Loring'' were stored 10 days and ''Elberta'' 20 days at 12 degrees C. ''Golden Delicious'' were stored 30 days at 20-25 degrees C in a dark room. Fruit were examined and differences in percentage rot and in physical-chemical properties determined. Percentage rot decreased with increasing UV dose. Fruit were firmer, pH and soluble solids lower and acidity higher for UV-treated than for nontreated peaches; pH was lower and acidity and ascorbic acid higher in UV treated than in nontreated apples. Percentage weight loss was less for UV-treated apples. The results indicated that UV treatment not only reduced storage rots but also delayed ripening of peaches and apples

A one-step multiplex RT-PCR assay for simultaneous detection of four viruses that infect peach.

Science.gov (United States)

Yu, Y; Zhao, Z; Jiang, D; Wu, Z; Li, S

2013-10-01

A multiplex reverse transcription polymerase chain reaction (mRT-PCR) assay was developed to enable the simultaneous detection and differentiation of four viruses that infect peach, namely Apple chlorotic leaf spot virus (ACLSV), Cherry green ring mottle virus (CGRMV), Prunus necrotic ringspot virus (PNRSV) and Apricot pseudo-chlorotic leaf spot virus (APCLSV). In this study, four pairs of primers, one specific for each virus, were designed; the corresponding PCR products were 632, 439, 346 and 282 bp in length for ACLSV, CGRMV, PNRSV and APCLSV, respectively, and the fragments could be distinguished clearly by agarose gel electrophoresis. The sensitivity and specificity of the method were tested using individual RT-PCR and enzyme-linked immunosorbent assay (ELISA), and the identity of the RT-PCR amplification products was also confirmed by DNA sequencing. The results of RT-PCR and ELISA, along with batch detection using samples collected from peach orchards, revealed that this rapid and simple technique is an effective way to identify the four viruses simultaneously. The mRT-PCR assay described in this study was developed for the simultaneous detection of four peach viruses from infected peach samples is reliable and sensitive. In contrast to conventional uniplex RT-PCR, mRT-PCR is more efficient, reducing costs, time and handling when testing large numbers of samples. This rapid and simple method is useful for large-scale surveys of viruses that infect peach. © 2013 The Society for Applied Microbiology.

Study of tritium permeation through Peach Bottom Steam Generator tubes

International Nuclear Information System (INIS)

Yang, L.; Baugh, W.A.; Baldwin, N.L.

1977-06-01

The report describes the equipment developed, samples tested, procedures used, and results obtained in the tritium permeation tests conducted on steam generator tubing samples which were removed from the Peach Bottom Unit No. 1 reactor

Contribution of pod borer pests to soybean crop production (case in Pondidaha, Konawe District, Southeast Sulawesi)

Science.gov (United States)

Rahayu, M.; Bande, LOS; Hasan, A.; Yuswana, A.; Rinambo, F.

2018-02-01

Soybean (Glycine max L.) is one of the most important crops which production continues to be improved in all areas of soybean cultivation centers in an effort to maintain the availability of soybean foods, including Southeast Sulawesi. The purpose of this study was to analyze the contribution of pod borer pests to soybean crop production. Methods of direct observation were made on observed variables, including species and population of pest pod borer, intensity, and crop production. The result that found four types of pod borer pests are Nezara viridula, Riptortus linearis, Etiella zinckenella, and Leptocorisa acuta, each with a different population and contribution to the intensity of pod damage. The result of path analysis showed that directly population of N. viridula (61.14) and E. zinckenella (66.44) gave positive contribution in increasing pod damage, by 0.332 and 0.502 respectively, while the negative contribution was shown by population of R. linearis and L. acuta. Damage of the pod causes increased production of low soybean is only about 0.202, therefore required appropriate control techniques to control pod borer pests populations in soybean crops.

Activity of pectin methyl esterase during blanching of peaches

NARCIS (Netherlands)

Tijskens, L.M.M.; Rodis, P.S.; Hertog, M.L.A.T.M.; Proxenia, N.; Dijk, van C.

1999-01-01

The activity of pectin methyl esterase (PE) in peaches during blanching treatments was modelled and analyzed. It was postulated that the enzyme exists in two configurations, one bound and one soluble. The bound configuration can be converted into the soluble configuration. These two configurations

Investigation of energy inputs for peach production using sensitivity analysis in Iran

International Nuclear Information System (INIS)

Royan, Mahsa; Khojastehpour, Mehdi; Emadi, Bagher; Mobtaker, Hassan Ghasemi

2012-01-01

Highlights: ► We investigated energy use and inputs–output relationship in peach production. ► Total energy consumption in peach production was 37536.96 MJ ha −1 . ► Diesel fuel with about (26.32%) was the major energy consumer. ► Energy use efficiency and energy productivity were 0.55 and 0.29 kg MJ −1 . ► The machinery energy was the most significant input affecting the output level. - Abstract: The purpose of this research was to investigate the energy balance between the energy inputs and yield in peach production in Golestan province of Iran as a case study. The results showed that total energy consumption in peach production was 37536.96 MJ ha −1 where the diesel fuel with about (26.32%) was the major energy consumer. The direct energy shared about (50.98%) whereas the indirect energy did (49.02%). Energy use efficiency, energy productivity, specific energy and net energy were 0.55, 0.29 kg MJ −1 , 3.41 MJ kg −1 and −16642.03 MJ ha −1 , respectively. Econometric assessment results revealed that the energy inputs of human labor, machinery, diesel fuel, chemical fertilizers and farm yard manure had significant influence on the yield. The impact of human labor energy (1.36) was found as the highest among the other input parameters. Sensitivity analysis indicated that the MPP value of energy inputs was between −2.8 and 11.31. Also the MPP value of human labor was the highest, followed by diesel fuel and farm yard manure energy inputs, respectively.

HPLC-DAD-ESIMS analysis of phenolic compounds in nectarines, peaches, and plums.

Science.gov (United States)

Tomás-Barberán, F A; Gil, M I; Cremin, P; Waterhouse, A L; Hess-Pierce, B; Kader, A A

2001-10-01

The phenolic compounds of 25 peach, nectarine, and plum cultivars were studied and quantified by HPLC-DAD-ESIMS. Hydroxycinnamates, procyanidins, flavonols, and anthocyanins were detected and quantified. White and yellow flesh nectarines and peaches, and yellow and red plums, were analyzed at two different maturity stages with consideration of both peel and flesh tissues. HPLC-MS analyses allowed the identification of procyanidin dimers of the B- and A-types, as well as the presence of procyanidin trimers in plums. As a general rule, the peel tissues contained higher amounts of phenolics, and anthocyanins and flavonols were almost exclusively located in this tissue. No clear differences in the phenolic content of nectarines and peaches were detected or between white flesh and yellow flesh cultivars. There was no clear trend in phenolic content with ripening of the different cultivars. Some cultivars, however, had a very high phenolic content. For example, the white flesh nectarine cultivar Brite Pearl (350-460 mg/kg hydroxycinnamates and 430-550 mg/kg procyanidins in flesh) and the yellow flesh cv. Red Jim (180-190 mg/kg hydroxycinnamates and 210-330 mg/kg procyanidins in flesh), contained 10 times more phenolics than cultivars such as Fire Pearl (38-50 mg/kg hydroxycinnamates and 23-30 mg/kg procyanidins in flesh). Among white flesh peaches, cultivars Snow King (300-320 mg/kg hydroxycinnamates and 660-695 mg/kg procyanidins in flesh) and Snow Giant (125-130 mg/kg hydroxycinnamates and 520-540 mg/kg procyanidins in flesh) showed the highest content. The plum cultivars Black Beaut and Angeleno were especially rich in phenolics.

Strategic removal of host trees in isolated, satellite infestations of emerald ash borer can reduce population growth

Science.gov (United States)

Samuel J. Fahrner; Mark Abrahamson; Robert C. Venette; Brian H. Aukema

2017-01-01

Emerald ash borer is an invasive beetle causing significant mortality of ash trees (Fraxinus spp.) in North America and western Russia. The invasive range has expanded to more than half of the states in the United States since the initial detection in Michigan, USA in 2002. Emerald ash borer is typically managed with a combination of techniques...

Cloning and expression of an endo-1,4-β-xylanase from the coffee berry borer, Hypothenemus hampei

Directory of Open Access Journals (Sweden)

Padilla-Hurtado Beatriz

2012-01-01

Full Text Available Abstract Background The coffee berry borer, Hypothenemus hampei, reproduces and feeds exclusively on the mature endosperm of the coffee seed, which has a cell wall composed mainly of a heterogeneous mixture of hemicellulose polysaccharides, including arabinoxylans. Xylanases are digestive enzymes responsible for the degradation of xylan based polymers, hydrolyzing them into smaller molecules that are easier to assimilate by insects. We report the cloning, expression and enzymatic characterization of a xylanase gene that was identified in the digestive tract of the coffee berry borer. Methods The complete DNA sequence encoding a H. hampei xylanase (HhXyl was obtained using a genome walking technique in a cDNA library derived from the borer digestive tract. The XIP-I gene was amplified from wheat (Triticum aestivum variety Soisson. A Pichia pastoris expression system was used to express the recombinant form of these enzymes. The xylanase activity and XIP-I inhibitory activity was quantified by the 3,5-dinitrosalicylic (DNS. The biological effects of XIP-I on borer individuals were evaluated by providing an artificial diet enriched with the recombinant XIP-I protein to the insects. Results The borer xylanase sequence contains a 951 bp open reading frame that is predicted to encode a 317-amino acid protein, with an estimated molecular weight of 34.92 kDa and a pI of 4.84. Bioinformatic analysis revealed that HhXyl exhibits high sequence homology with endo-β-D-xylanases of Streptomyces bingchenggensis from glycosyl hydrolase 10 (GH10. The recombinant xylanase showed maximal activity at pH 5.5 and 37°C. XIP-I expressed as a recombinant protein inhibited HhXyl activity in vitro and caused individual H. hampei mortality in bioassays when included as a supplement in artificial diets. Conclusion A xylanase from the digestive tract of the coffee berry borer was identified and functionally characterized. A xylanase inhibitor protein, XIP-I, from wheat was

Primary coolant chemistry of the Peach Bottom and Fort St. Vrain high-temperature gas-cooled reactors

International Nuclear Information System (INIS)

Burnette, R.D.; Baldwin, N.L.

1980-11-01

The chemical impurities in the primary coolants of the Peach Bottom and Fort St. Vrain reactors are discussed. The impurity mixtures in the two plants were quite different because the sources of the impurities were different. In the Peach Bottom reactor, the impurities were dominated by H 2 and CH 4 , which are decomposition products of oil. In the Fort St. Vrain reactor, there were high levels of CO, CO 2 , and H 2 O. Although oil ingress at Peach Bottom created carbon deposits on virtually all surfaces, its effect on reactor operation was negligible. Slow outgassing of water from the thermal insulation at Fort St. Vrain caused delays in reactor startup. The overall graphite oxidation in both plants was negligible

Primary coolant chemistry of the Peach Bottom and Fort St. Vrain high temperature gas-cooled reactors

International Nuclear Information System (INIS)

Burnette, R.D.; Baldwin, N.L.

1981-01-01

The chemical impurities in the primary coolants of the Peach Bottom and Fort St. Vrain reactors are discussed. The impurity mixtures in the two plants were quite different because the sources of the impurities were different. In the Peach Bottom reactor, the impurities were dominated by H 2 and CH 4 , which are decomposition products of oil. In the Fort St. Vrain reactor, there were high levels of CO, CO 2 , and H 2 O. Although oil ingress at Peach Bottom created carbon deposits on virtually all surfaces, its effect on reactor operation was negligible. Slow outgassing of water from the thermal insulation at Fort St. Vrain caused delays in reactor startup. The overall graphite oxidation in both plants was negligible. (author)

Characterising root density of peach trees in a semi-arid Chernozem to increase plant density

Science.gov (United States)

Paltineanu, Cristian; Septar, Leinar; Gavat, Corina; Chitu, Emil; Oprita, Alexandru; Moale, Cristina; Calciu, Irina; Vizitiu, Olga; Lamureanu, Gheorghe

2016-01-01

The available information on root system in fully mature peach orchards in semi-arid regions is insufficient. This paper presents a study on the root system density in an irrigated peach orchard from Dobrogea, Romania, using the trench technique. The old orchard has clean cultivation in inter-row and in-row. The objectives of the study were to: test the hypothesis that the roots of fully mature peach trees occupy the whole soil volume; find out if root repulsive effect of adjacent plants occurred for the rootstocks and soil conditions; find relationships between root system and soil properties and analyse soil state trend. Some soil physical properties were significantly deteriorated in inter-row versus in-row, mainly due to soil compaction induced by technological traffic. Density of total roots was higher in-row than inter-row, but the differences were not significant. Root density decreased more intensely with soil depth than with distance from tree trunks. Root density correlated with some soil properties. No repulsive effect of the roots of adjacent peach trees was noted. The decrease of root density with distance from trunk can be used in optimising tree arrangement. The conclusions could also be used in countries with similar growth conditions.

75 FR 3694 - Radio Broadcasting Services, Peach Springs, Arizona

Science.gov (United States)

2010-01-22

...., Fletcher, Heald & Hildreth, PLC, 1300 N. 17th Street - Eleventh Floor, Arlington, Virginia 22209 (Counsel... website, http://www.bcpiweb.com >. The proposed channel at Peach Springs is part of a hybrid application and rulemaking proceeding. In the application (File No. BNPH-20091016ADO), Cochise Media Licenses, the...

Effect of the addition of peach palm (Bactris gasipaes peel flour on the color and sensory properties of cakes

Directory of Open Access Journals (Sweden)

Jader MARTÍNEZ-GIRÓN

Full Text Available Abstract This study aimed to evaluate the color and sensorial characteristics of a cake made with different amounts of flour made with peach palm peel flour. The flour was added at different concentrations, 2.5, 5.0, 7.5, and 10% (w/w, including a control (tartrazine. Physiochemical analyses were carried out on the cakes, including total carotenoids, CIE-L*a*b* color coordinates, and sensorial attributes. The results demonstrated that the peach palm peel flour substitution increased the total carotenoid content of the cakes. The color test showed that high levels of peach palm peel flour resulted in a decrease in the values of lightness (L* and hue angle (h°, while the values of browning index (BI and color change (ΔE* increased significantly. The qualification of the sensorial evaluation indicated that the quality attributes were acceptable in the cakes made with wheat flour and 7.5% peach palm peel flour. Therefore, it was concluded that flour obtained from peach palm peel is a by-product that can be used as a natural food dye alternative in bread products.

Control of Busseola fusca and Chilo partellus stem borers by ...

African Journals Online (AJOL)

GREGORY

2011-06-01

Jun 1, 2011 ... 1International Maize and Wheat Improvement Center, P.O. Box 1041 ... Key words: Bacillus thuringiensis (Bt) maize, cry1A (b) proteins, stem borers, transgenic. ... including conservation agriculture on insect pests, can only be ...

Appraisal of genetic diversity of different peach cultivars and genotypes through rapd markers

International Nuclear Information System (INIS)

Bakht, J.; Jamal, N.; Shafi, M

2012-01-01

The present study was amid to investigate the genetic diversity of twenty peach cultivars and genotypes by RAPD primers at the Institute of Biotechnology and Genetic Engineering, KPK Agricultural University Peshawar. The result indicated that fifteen primers (GLCO9, GLC20, GLA20, GLA13, GLB10, GLB20, GLB06, GLB19, GLA19, GLB19, GLD16, GLB15, GLA15, GLB12, GLB11) gave genetic distance among the peach cultivars and genotypes under study by PCR amplification. Average genetic diversity (estimated as genetic distance) ranged between 12 and 58%. The molecular size of most of the bands were from 150 bp to 1000 bp. Based on dendrogram analysis, Khyber 1 and Khyber 2 was grouped in cluster A, and Tex-A6-69 and BY-8-135 in cluster B, Candan and 6A were most closely related cultivars and genotypes among the 20 peach cultivars and genotypes while Lering, Flam crest, Tex x-9, early grand and Floradaking were distinctly grouped when compared with the rest of population. (author)

Non-destructive grading of peaches by near-infrared spectrometry

Science.gov (United States)

Carlomagno, G.; Capozzo, L.; Attolico, G.; Distante, A.

2004-12-01

This paper describes an experimental study on non-destructive methods for sorting peaches according to their degree of ripeness. The method is based on near-infrared (NIR) transmittance spectrometry in the region between 730 and 900 nm. It estimates the ripeness in terms of internal sugar content and firmness. A station for acquiring the NIR signal has been designed and realized, carefully choosing between several options for each component. Four different stations have been realized and compared during the experimental phase. The signals acquired by the station have been pre-processed using a noise-reducing method based on a packets-wavelet transform. In addition, an outlier detection technique has been applied for identifying irregular behaviors inside each of the considered classes. Finally, a minimum distance classifier estimates the grade of each experimental data. The results obtained in classification show that this early version of the station enables the correct discrimination of peaches with a percentage of 82.5%.

Ultrasound Assisted Extraction of Phenolic Compounds from Peaches and Pumpkins

Science.gov (United States)

Altemimi, Ammar; Watson, Dennis G.; Choudhary, Ruplal; Dasari, Mallika R.; Lightfoot, David A.

2016-01-01

The ultrasound-assisted extraction (UAE) method was used to optimize the extraction of phenolic compounds from pumpkins and peaches. The response surface methodology (RSM) was used to study the effects of three independent variables each with three treatments. They included extraction temperatures (30, 40 and 50°C), ultrasonic power levels (30, 50 and 70%) and extraction times (10, 20 and 30 min). The optimal conditions for extractions of total phenolics from pumpkins were inferred to be a temperature of 41.45°C, a power of 44.60% and a time of 25.67 min. However, an extraction temperature of 40.99°C, power of 56.01% and time of 25.71 min was optimal for recovery of free radical scavenging activity (measured by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) reduction). The optimal conditions for peach extracts were an extraction temperature of 41.53°C, power of 43.99% and time of 27.86 min for total phenolics. However, an extraction temperature of 41.60°C, power of 44.88% and time of 27.49 min was optimal for free radical scavenging activity (judged by from DPPH reduction). Further, the UAE processes were significantly better than solvent extractions without ultrasound. By electron microscopy it was concluded that ultrasonic processing caused damage in cells for all treated samples (pumpkin, peach). However, the FTIR spectra did not show any significant changes in chemical structures caused by either ultrasonic processing or solvent extraction. PMID:26885655

Relationship between endogenous hormonal content and somatic organogenesis in callus of peach (Prunus persica L. Batsch) cultivars and Prunus persica×Prunus dulcis rootstocks.

Science.gov (United States)

Pérez-Jiménez, Margarita; Cantero-Navarro, Elena; Pérez-Alfocea, Francisco; Le-Disquet, Isabel; Guivarc'h, Anne; Cos-Terrer, José

2014-05-01

The relationship between endogenous hormones content and the induction of somatic peach plant was studied. To induce multiple shoots from callus derived from the base of stem explants of the scion cultivars 'UFO-3', 'Flariba' and 'Alice Bigi', and the peach×almond rootstocks 'Garnem' and 'GF677', propagated plants were cultured on Murashige and Skoog salts augmented with 0.1mgL(-1) of indolebutyric acid, 1mgL(-1) of 6-benzylaminopurine and 3% sucrose. The highest regeneration rate was obtained with the peach×almond rootstocks. Endogenous levels of abscisic acid (ABA), indole-3-acetic acid (IAA), zeatin (Z), zeatin riboside (ZR), ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), salicylic acid (SA), and jasmonic acid (JA) were analyzed in the organogenic callus. Lower levels of several hormones, namely Z, ZR, ABA, and ACC were found in the peach×almond rootstock compared to peach cultivars, while IAA and SA presented inconclusive returns. These results suggest that the difference in somatic organogenesis capacity observed in peach and peach×almond hybrids is markedly affected by the endogenous hormonal content of the studied genotypes. Copyright © 2014 Elsevier GmbH. All rights reserved.

Improving detection tools for the emerald ash borer (Coleoptera: Buprestidae): comparison of prism and multifunnel traps at varying population densities.

Science.gov (United States)

Francese, Joseph A; Rietz, Michael L; Crook, Damon J; Fraser, Ivich; Lance, David R; Mastro, Victor C

2013-12-01

The current emerald ash borer survey trap used in the United States is a prism trap constructed from a stock purple corrugated plastic. In recent years, several colors (particularly shades of green and purple) have been shown to be more attractive to the emerald ash borer than this stock color. Our goal was to determine if plastics produced with these colors and incorporated into prism traps can improve and serve as a new alternative to plastics already in use for the emerald ash borer survey. The plastics were tested in moderate to heavily infested areas in Michigan in two initial studies to test their effectiveness at catching the emerald ash borer. Because results from studies performed in heavily infested sites may not always correspond with what is found along the edges of the infestation, we compared trap catch and detection rates (recording at least one catch on a trap over the course of the entire trapping season) of several trap types and colors at sites outside the core of the currently known emerald ash borer infestation in a nine-state detection tool comparison study. Two of the new plastics, a (Sabic) purple and a medium-dark (Sabic) green were incorporated into prism traps and tested alongside a standard purple prism trap and a green multifunnel trap. In areas with lower emerald ash borer density, the new purple (Sabic) corrugated plastic caught more beetles than the current purple prism trap, as well as more than the medium-dark green (Sabic) prism and green multifunnel traps. Sabic purple traps in the detection tools comparison study recorded a detection rate of 86% compared with 73, 66, and 58% for the standard purple, Sabic green, and green multifunnel traps, respectively. These detection rates were reduced to 80, 63, 55, and 46%, respectively, at low emerald ash borer density sites.

Implications of Black Coffee Twig Borer on cocoa in Uganda

African Journals Online (AJOL)

ACSS

2014a,b). This pest thus poses a serious threat to both coffee and cocoa production in Uganda, and therefore, calls for prompt comprehensive mitigation actions (Kagezi et al., 2013a,b, 2014a,b,c,d). Damage is caused by the female beetle by boring a characteristic pin-sized entry hole into the attacked seedlings and/or.

Ecological and physiological aspects of aestivation-diapause in the larvae of two Pyralid stalk borers of maize in Kenya

NARCIS (Netherlands)

Scheltes, P.

1978-01-01

Stalk borers are highly destructive to a large number of important graminaceous crops all over the world. Some examples of economically important stalk borers and a general description of their life-cycle are mentioned in chapter 1. In the same chapter difficulties in controlling the insects are

Phylogeography of the walnut twig beetle, Pityophthorus juglandis, the vector of thousand cankers disease in North American walnut trees

Science.gov (United States)

Paul F. Rugman-Jones; Steven J. Seybold; Andrew D. Graves; Richard. Stouthamer

2015-01-01

Thousand cankers disease (TCD) of walnut trees (Juglans spp.) results from aggressive feeding in the phloem by the walnut twig beetle (WTB), Pityophthorus juglandis, accompanied by inoculation of its galleries with a pathogenic fungus, Geosmithia morbida. In 1960, WTB was only known from four U.S. counties...

Invasion genetics of emerald ash borer in North America

Science.gov (United States)

Alicia M. Bray; Leah S. Bauer; Robert A. Haack; Therese Poland; James J. Smith

2006-01-01

Emerald ash borer (EAB) was first detected in Michigan and Canada in 2002. Efforts to eradicate this destructive pest by federal and state regulatory agencies continue. Knowledge of EAB genetics will be useful in understanding the invasion dynamics of the beetle and to help identify geographic localities of potential biocontrol agents.

Comparative Transcriptome and Microscopy Analyses Provide Insights into Flat Shape Formation in Peach (Prunus persica

Directory of Open Access Journals (Sweden)

Jian Guo

2018-01-01

Full Text Available Fruit shape is an important external characteristic that consumers use to select preferred fruit cultivars. In peach, the flat fruit cultivars have become more and more popular worldwide. Genetic markers closely linking to the flat fruit trait have been identified and are useful for marker-assisted breeding. However, the cellular and genetic mechanisms underpinning flat fruit formation are still poorly understood. In this study, we have revealed the differences in fruit cell number, cell size, and in gene expression pattern between the traditional round fruit and modern flat fruit cultivars. Flat peach cultivars possessed significantly lower number of cells in the vertical axis because cell division in the vertical direction stopped early in the flat fruit cultivars at 15 DAFB (day after full bloom than in round fruit cultivars at 35 DAFB. This resulted in the reduction in vertical development in the flat fruit. Significant linear relationship was observed between fruit vertical diameter and cell number in vertical axis for the four examined peach cultivars (R2 = 0.9964 at maturation stage, and was also observed between fruit vertical diameter and fruit weight (R2 = 0.9605, which indicated that cell number in vertical direction contributed to the flat shape formation. Furthermore, in RNA-seq analysis, 4165 differentially expressed genes (DEGs were detected by comparing RNA-seq data between flat and round peach cultivars at different fruit development stages. In contrast to previous studies, we discovered 28 candidate genes potentially responsible for the flat shape formation, including 19 located in the mapping site and 9 downstream genes. Our study indicates that flat and round fruit shape in peach is primarily determined by the regulation of cell production in the vertical direction during early fruit development.

Introgression between divergent corn borer species in a region of sympatry: implications on the evolution and adaptation of pest arthropods

Science.gov (United States)

The Asian corn borer, Ostrinia furnacalis, and European corn borer, O. nubilalis (Lepidoptera: Crambidae) cause damage to cultivated maize in spatially distinct geographies, and have evolved divergent hydrocarbons as the basis of sexual communication. The Yili area of Xinjiang Province China repres...

Lambda-cyhalothrin efficiency on fruit borer control and quali-quantitative spraying aspects in a pinecone crop

OpenAIRE

Jacqueline Lavinscky Costa Morais; Maria Aparecida Castellani; Carlos Gilberto Raetano; Juliana Alves de Macêdo; Moisés Silva Nery; Gabriela Luz Pereira Moreira

2016-01-01

ABSTRACT In Brazil, the state of Bahia is one of the largest pinecone (Annona squamosa L.) growers; nevertheless, fruit borer (Cerconota anonella L.) presence limits production. This research aimed to test the efficiency of lambda-cyhalothrin in controlling fruit borer using different spray volumes; additionally, this research tested qualitative and quantitative operational aspects. Trials were carried out in pinecone orchards in Caraíbas-BA, Brazil. Pesticide efficiency was tested by a rando...

Micropropagation of peach rootstocks and cultivars.

Science.gov (United States)

Balla, Ildikó; Mansvelt, Lucienne

2013-01-01

Peach (Prunus persica (L.) Batsch) is one of the most popular stone fruits, commercially produced largely in Mediterranean and, to a lesser extent, in continental climatic conditions. Several breeding programs with different aims release annually large numbers of new cultivars. Micropropagation offers a suitable method to provide the growers of sufficient quantities of rootstocks, as well as of pathogen-free planting material of old and new cultivars.An effective four-step micropropagation procedure for cultivar and rootstock production is described here, based on the use of modified MS and WPM media. The health status of the initial shoot tips is very important, also because the growth and proliferation rate of shoot cultures from virus-infected clones are generally very poor. Proliferation and elongation phases depend on the major macro-elements, as well as the content and ratio of plant growth regulators. It is important to grow the cultures at 22°C, as hyperhydricity may develop at higher temperatures. Although sucrose is the most common carbon source used during proliferation and rooting, for some peach cultivars and rootstocks the replacement of sucrose (10 g/L) with glucose (20 g/L) in the rooting medium improves the rooting and survival rates of plants in the acclimatization phase. The rooting rate of the rootstock "Cadaman" is improved with the chelated form of iron FeEDDHA at 150 mg/L. Rooted plants are acclimatized in greenhouse under high humidity conditions.

Optimizing Use of Girdled Ash Trees for Management of Low-Density Emerald Ash Borer (Coleoptera: Buprestidae) Populations.

Science.gov (United States)

Siegert, Nathan W; McCullough, Deborah G; Poland, Therese M; Heyd, Robert L

2017-06-01

Effective survey methods to detect and monitor recently established, low-density infestations of emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), remain a high priority because they provide land managers and property owners with time to implement tactics to slow emerald ash borer population growth and the progression of ash mortality. We evaluated options for using girdled ash (Fraxinus spp.) trees for emerald ash borer detection and management in a low-density infestation in a forested area with abundant green ash (F. pennsylvanica). Across replicated 4-ha plots, we compared detection efficiency of 4 versus 16 evenly distributed girdled ash trees and between clusters of 3 versus 12 girdled trees. We also examined within-tree larval distribution in 208 girdled and nongirdled trees and assessed adult emerald ash borer emergence from detection trees felled 11 mo after girdling and left on site. Overall, current-year larvae were present in 85-97% of girdled trees and 57-72% of nongirdled trees, and larval density was 2-5 times greater on girdled than nongirdled trees. Low-density emerald ash borer infestations were readily detected with four girdled trees per 4-ha, and 3-tree clusters were as effective as 12-tree clusters. Larval densities were greatest 0.5 ± 0.4 m below the base of the canopy in girdled trees and 1.3 ± 0.7 m above the canopy base in nongirdled trees. Relatively few adult emerald ash borer emerged from trees felled 11 mo after girdling and left on site through the following summer, suggesting removal or destruction of girdled ash trees may be unnecessary. This could potentially reduce survey costs, particularly in forested areas with poor accessibility. Published by Oxford University Press on behalf of Entomological Society of America 2017. This work is written by US Government employees and is in the public domain in the US.

Deficit irrigation of peach trees to reduce water consumption

Science.gov (United States)

Lack of water is a major limiting factor for production tree fruits such as peaches in the San Joaquin Valley of California and many other arid- or semi-arid regions in the world. Deficit irrigation can be used in some cropping systems as a water resource management strategy to reduce non-productiv...

Version VI of the ESTree db: an improved tool for peach transcriptome analysis

Science.gov (United States)

Lazzari, Barbara; Caprera, Andrea; Vecchietti, Alberto; Merelli, Ivan; Barale, Francesca; Milanesi, Luciano; Stella, Alessandra; Pozzi, Carlo

2008-01-01

Background The ESTree database (db) is a collection of Prunus persica and Prunus dulcis EST sequences that in its current version encompasses 75,404 sequences from 3 almond and 19 peach libraries. Nine peach genotypes and four peach tissues are represented, from four fruit developmental stages. The aim of this work was to implement the already existing ESTree db by adding new sequences and analysis programs. Particular care was given to the implementation of the web interface, that allows querying each of the database features. Results A Perl modular pipeline is the backbone of sequence analysis in the ESTree db project. Outputs obtained during the pipeline steps are automatically arrayed into the fields of a MySQL database. Apart from standard clustering and annotation analyses, version VI of the ESTree db encompasses new tools for tandem repeat identification, annotation against genomic Rosaceae sequences, and positioning on the database of oligomer sequences that were used in a peach microarray study. Furthermore, known protein patterns and motifs were identified by comparison to PROSITE. Based on data retrieved from sequence annotation against the UniProtKB database, a script was prepared to track positions of homologous hits on the GO tree and build statistics on the ontologies distribution in GO functional categories. EST mapping data were also integrated in the database. The PHP-based web interface was upgraded and extended. The aim of the authors was to enable querying the database according to all the biological aspects that can be investigated from the analysis of data available in the ESTree db. This is achieved by allowing multiple searches on logical subsets of sequences that represent different biological situations or features. Conclusions The version VI of ESTree db offers a broad overview on peach gene expression. Sequence analyses results contained in the database, extensively linked to external related resources, represent a large amount of

Evaluation of peach palm (Bactris gasipaes Kunth) processed by radiation

International Nuclear Information System (INIS)

Silva, Priscila Vieira da

2009-01-01

The peach palm can be obtained from several species of palms, but the peach palm has attracted great interest by producers, as has characteristics of precocity, rusticity and tillering, producing a palm-quality differentiating it from other palmettos for their sweet flavor and yellowish . The food irradiation has been used as a treatment to ensure microbiological food safety of products to avoid infection. Its use combined with minimal processing could increase the safety and quality of minimally processed vegetables. We aimed at evaluating the effect of gamma radiation and electron beams to control bacteria; assess the physical characteristics through analysis of color and texture in peach palm in natura minimally processed and subjected to ionizing radiation stored at 8 deg C as well as evaluating the sensory characteristics. The results in the microbiological analysis showed that ionizing radiation promotes reduction of microbial load in both treatments. In the analysis of color we can conclude that among all the treatments the sample irradiated with 1.5 kGy showed more differences when compared with the other samples. Observing texture characteristics we could conclude that irradiation changed the texture of the palm, unlike the treatment by electron beams that showed no difference between samples. For the sensory analysis, the gamma radiation with dose of 1.5 kGy, induced changes in sensory properties to the attributes and overall appearance. The dose of 1 kGy caused no significant difference, so a recommended dose for the irradiation of the studied product. (author)

An integrated approach for increasing breeding efficiency in apple and peach in Europe.

Science.gov (United States)

Laurens, Francois; Aranzana, Maria José; Arus, Pere; Bassi, Daniele; Bink, Marco; Bonany, Joan; Caprera, Andrea; Corelli-Grappadelli, Luca; Costes, Evelyne; Durel, Charles-Eric; Mauroux, Jehan-Baptiste; Muranty, Hélène; Nazzicari, Nelson; Pascal, Thierry; Patocchi, Andrea; Peil, Andreas; Quilot-Turion, Bénédicte; Rossini, Laura; Stella, Alessandra; Troggio, Michela; Velasco, Riccardo; van de Weg, Eric

2018-01-01

Despite the availability of whole genome sequences of apple and peach, there has been a considerable gap between genomics and breeding. To bridge the gap, the European Union funded the FruitBreedomics project (March 2011 to August 2015) involving 28 research institutes and private companies. Three complementary approaches were pursued: (i) tool and software development, (ii) deciphering genetic control of main horticultural traits taking into account allelic diversity and (iii) developing plant materials, tools and methodologies for breeders. Decisive breakthroughs were made including the making available of ready-to-go DNA diagnostic tests for Marker Assisted Breeding, development of new, dense SNP arrays in apple and peach, new phenotypic methods for some complex traits, software for gene/QTL discovery on breeding germplasm via Pedigree Based Analysis (PBA). This resulted in the discovery of highly predictive molecular markers for traits of horticultural interest via PBA and via Genome Wide Association Studies (GWAS) on several European genebank collections. FruitBreedomics also developed pre-breeding plant materials in which multiple sources of resistance were pyramided and software that can support breeders in their selection activities. Through FruitBreedomics, significant progresses were made in the field of apple and peach breeding, genetics, genomics and bioinformatics of which advantage will be made by breeders, germplasm curators and scientists. A major part of the data collected during the project has been stored in the FruitBreedomics database and has been made available to the public. This review covers the scientific discoveries made in this major endeavour, and perspective in the apple and peach breeding and genomics in Europe and beyond.

Breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective

Directory of Open Access Journals (Sweden)

Basilio Carrasco

2013-01-01

Full Text Available This review is an overview of traditional and modern breeding methodologies being used to develop new Prunus cultivars (stone fruits with major emphasis on peach, sweet cherry and Japanese plum. To this end, common breeding tools used to produce seedlings, including in vitro culture tools, are discussed. Additionally, the mechanisms of inheritance of many important agronomical traits are described. Recent advances in stone fruit transcriptomics and genomic resources are providing an understanding of the molecular basis of phenotypic variability as well as the identification of allelic variants and molecular markers. These have potential applications for understanding the genetic diversity of the Prunus species, molecular marker-assisted selection and transgenesis. Simple Sequence Repeat (SSR and Single Nucleotide Polymorphism (SNPs molecular markers are described as useful tools to describe genetic diversity in peach, sweet cherry and Japanese plum. Additionally, the recently sequenced peach genome and the public release of the sweet cherry genome are discussed in terms of their applicability to breeding programs

Optimization of visual trapping methodology for the emerald ash borer, Agrilus planipennis

Science.gov (United States)

Joseph A. Francese; Damon J. Crook; Ivich Fraser; David R. Lance; Alan J. Sawyer; Victor C. Mastro

2009-01-01

As the emerald ash borer (EAB), Agrilus planipennis (Coleoptera: Buprestidae), spreads throughout the range of North American ash species, better tools are needed for the detection and delimitation of new infestations...

Gas exchanges in peach palms as a function of the spad chlorophyll meter readings

Directory of Open Access Journals (Sweden)

Maria Luiza Sant'anna Tucci

2011-10-01

Full Text Available The close relationship between the chlorophyll-meters readings and the total chlorophyll and nitrogen contents in leaves, has allowed their evaluation both in annual and perennial species. Besides, some physiological events such as the CO2 assimilation have also been estimated by chlorophyll meters. This work was carried out aiming to evaluate the gas exchanges of peach palms as a function of the chlorophyll SPAD-Meter readings. Three year-old peach palms from Yurimaguas, Peru were studied in Ubatuba, SP, Brazil, spaced 2 x 1 m in area under a natural gradient of organic matter which allowed four plots to be considered, according to the peach palms leaves colors, from light yellow to dark green. The SPAD readings and the stomatal frequency of leaflets were evaluated. The photosynthetic photon flux density (PPFD, μmol m-2 s-1, the leaf temperature (Tleaf, ºC, the CO2 assimilation (A, μmol m-2 s-1, the stomatal conductance (g s, mol m-2 s-1, the transpiration (E, mmol m-2 s-1 and the intercellular CO2 concentration (Ci, μmol mol-1 were evaluated with a portable infrared gas analyzer (LCA-4, ADC BioScientific Ltd., Great Amwell, U.K.. A linear increase in the CO2 assimilation as a function of the SPAD readings (y = -0.34 + 0.19x, R² = 0.99, indicates that they can be a rapid and cheap complementary method to evaluate in peach palms some important physiological events, such as CO2 assimilation.

Development of kairomone based control programs for cocoa pod borer

Science.gov (United States)

The Cocoa Pod Borer moth presents a unique opportunity to develop host volatile attractants for control strategies for the following reasons. First, knowing what volatiles are critical for host finding by females will allow for development of mass trapping and/or attract and kill strategies to cont...

Effects of Bacillus thuringiensis SDS-502 on adult emerald ash borer

Science.gov (United States)

Leah S. Bauer; Diana K. Londo& #241; o

2011-01-01

Emerald ash borer (EAB), Agrilus planipennis, an intermittent pest of ash (Fraxinus) trees in northeastern Asia, was discovered in Michigan and Ontario in 2002. In North America, infestations of EAB are now known in 13 states and 2 provinces.

Identification of differentially expressed genes associated with changes in the morphology of Pichia fermentans on apple and peach fruit.

Science.gov (United States)

Fiori, Stefano; Scherm, Barbara; Liu, Jia; Farrell, Robert; Mannazzu, Ilaria; Budroni, Marilena; Maserti, Bianca E; Wisniewski, Michael E; Migheli, Quirico

2012-11-01

Pichia fermentans (strain DISAABA 726) is an effective biocontrol agent against Monilinia fructicola and Botrytis cinerea when inoculated in artificially wounded apple fruit but is an aggressive pathogen when inoculated on wounded peach fruit, causing severe fruit decay. Pichia fermentans grows as budding yeast on apple tissue and exhibits pseudohyphal growth on peach tissue, suggesting that dimorphism may be associated with pathogenicity. Two complementary suppressive subtractive hybridization (SSH) strategies, that is, rapid subtraction hybridization (RaSH) and PCR-based subtraction, were performed to identify genes differentially expressed by P. fermentans after 24-h growth on apple vs. peach fruit. Gene products that were more highly expressed on peach than on apple tissue, or vice versa, were sequenced and compared with available yeast genome sequence databases. Several of the genes more highly expressed, when P. fermentans was grown on peach, were related to stress response, glycolysis, amino acid metabolism, and alcoholic fermentation but surprisingly not to cell wall degrading enzymes such as pectinases or cellulases. The dual activity of P. fermentans as both a biocontrol agent and a pathogen emphasizes the need for a thorough risk analysis of potential antagonists to avoid unpredictable results that could negatively impact the safe use of postharvest biocontrol strategies. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

Evaluating rapid response to a goldspotted oak borer diaspora

Science.gov (United States)

Tom Scott; Kevin Turner

2015-01-01

In 2012, the goldspotted oak borer (Agrilus auroguttatus, GSOB) was discovered in the mountain community of Idyllwild, 56.3 km north of its known area of infestation. This was the third time that a point of outbreak was discovered >32.2 km from the GSOB infestation area, suggesting that human transport of GSOB has substantially expanded the...

Is home-based palliative care cost-effective? An economic evaluation of the Palliative Care Extended Packages at Home (PEACH) pilot.

Science.gov (United States)

McCaffrey, Nikki; Agar, Meera; Harlum, Janeane; Karnon, Jonathon; Currow, David; Eckermann, Simon

2013-12-01

The aim of this study was to evaluate the cost-effectiveness of a home-based palliative care model relative to usual care in expediting discharge or enabling patients to remain at home. Economic evaluation of a pilot randomised controlled trial with 28 days follow-up. Mean costs and effectiveness were calculated for the Palliative Care Extended Packages at Home (PEACH) and usual care arms including: days at home; place of death; PEACH intervention costs; specialist palliative care service use; acute hospital and palliative care unit inpatient stays; and outpatient visits. PEACH mean intervention costs per patient ($3489) were largely offset by lower mean inpatient care costs ($2450) and in this arm, participants were at home for one additional day on average. Consequently, PEACH is cost-effective relative to usual care when the threshold value for one extra day at home exceeds $1068, or $2547 if only within-study days of hospital admission are costed. All estimates are high uncertainty. The results of this small pilot study point to the potential of PEACH as a cost-effective end-of-life care model relative to usual care. Findings support the feasibility of conducting a definitive, fully powered study with longer follow-up and comprehensive economic evaluation.

Differential fipronil susceptibility and metabolism in two rice stem borers from China.

Science.gov (United States)

Fang, Qi; Huang, Cheng-Hua; Ye, Gong-Yin; Yao, Hong-Wei; Cheng, Jia-An; Akhtar, Zunnu-Raen

2008-08-01

The susceptibilities of larvae of two rice stem borers, namely, Chilo suppressalis (Walker) (Lepidoptera: Crambidae) and Sesamia inferens (Walker) (Lepidoptera: Nocutidae) to fipronil and its metabolites were investigated, and then the activities of microsomal O-demethylase, and glutathione transferase (GST) in two species were measured. The metabolism of fipronil in both stem borers was determined in vivo and in vitro. The LD50 value of fipronil to S. inferens was 118.5-fold higher than that of C. suppressalis. The bioassay results offipronil metabolites showed that the toxicities of sulfone and sulfide were higher than fipronil for both species, and the differential toxicity between sulfone and fipronil was remarkable. Alternatively, the activities of microsomal O-demethylase and GST of C. suppressalis were 1.35- and 2.06-fold higher than S. inferens, respectively. The in vivo and in vitro studies on metabolism of fipronil showed that all of fipronil, sulfone, and sulfide were detected and the content of sulfone was higher than sulfide in both stem borers. The residue of sulfone in C. suppressalis was significantly higher than that in S. inferens. These results suggest that the higher activity of mixed function oxidases may cause the higher capacity of C. suppressalis to produce fipronil-sulfone, which is more toxic than fipronil leading to the higher susceptibility of this species.

Effect of transgenic Bacillus thuringiensis rice lines on mortality and feeding behavior of rice stem borers (Lepidoptera: Crambidae).

Science.gov (United States)

Chen, Hao; Zhang, Guoan; Zhang, Qifa; Lin, Yongjun

2008-02-01

Ten transgenic Bacillus thuringiensis Bt rice, Oryza sativa L., lines with different Bt genes (two Cry1Ac lines, three Cry2A lines, and five Cry9C lines) derived from the same variety Minghui 63 were evaluated in both the laboratory and the field. Bioassays were conducted by using the first instars of two main rice lepidopteran insect species: yellow stem borer, Scirpophaga incertulas (Walker) and Asiatic rice borer, Chilo suppressalis (Walker). All transgenic lines exhibited high toxicity to these two rice borers. Field evaluation results also showed that all transgenic lines were highly insect resistant with both natural infestation and manual infestation of the neonate larvae of S. incertulas compared with the nontransformed Minghui63. Bt protein concentrations in leaves of 10 transgenic rice lines were estimated by the sandwich enzyme-linked immunosorbent assay. The cry9C gene had the highest expression level, next was cry2A gene, and the cry1Ac gene expressed at the lowest level. The feeding behavior of 7-d-old Asiatic rice borer to three classes of Bt transgenic rice lines also was detected by using rice culm cuttings. The results showed that 7-d-old larvae of Asiatic rice borer have the capacity to distinguish Bt and non-Bt culm cuttings and preferentially fed on non-Bt cuttings. When only Bt culm cuttings with three classes of different Bt proteins (CrylAc, Cry2A, and Cry9C) were fed, significant distribution difference of 7-d-old Asiatic rice borer in culm cuttings of different Bt proteins also was found. In the current study, we evaluate different Bt genes in the same rice variety in both the laboratory and the field, and also tested feeding behavior of rice insect to these Bt rice. These data are valuable for the further development of two-toxin Bt rice and establishment of appropriate insect resistance management in the future.

Spatial and temporal patterns of xylem sap pH derived from stems and twigs of Populus deltoides L.

Science.gov (United States)

Doug Aubrey; Justin Boyles; Laura Krysinsky; Robert Teskey

2011-01-01

Xylem sap pH (pHX) is critical in determining the quantity of inorganic carbon dissolved in xylem solution from gaseous [CO2] measurements. Studies of internal carbon transport have generally assumed that pHX derived from stems and twigs is similar and that pHX remains constant through time; however, no empirical studies have investigated these assumptions. If any of...

Effect of controlled atmosphere on postharvest quality of 'Douradão' peaches

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Ligia Regina Radomille de Santana

2011-03-01

Full Text Available This study was carried out with one of the most important cultivar grown in the State of Sao Paulo, Brazil, which has gained the preference of consumers, due to its sweet taste, intense skin color and large size; however, these fruits are susceptible to chilling injury when cold stored for long periods. The use of controlled atmosphere (CA with elevated CO2 and reduced O2 concentrations prevent the onset of the chilling symptom. Thus, the effect of three different conditions of controlled atmosphere (CA1, CA2, CA3 and Control was evaluated in order to extend the storage life of 'Douradão' peaches. After 14, 21 and 28 days, samples were withdrawn from CA and kept in fresh air at 25 ± 1 °C and 90 ± 5% RH to complete ripening. On the day of removal and after 4 days, were the peaches quality characteristics were evaluated. The results showed that the use of CA during cold storage reduced weight loss and prevented postharvest decay. CA2 and CA3 treatments were effective in keeping good quality of 'Douradão' peaches during 28 days of cold storage, the ripe fruits showed reduced incidence of woolliness, adequate juiciness and flesh firmness. CA1 and Control treatments did not present marketable conditions after 14 days of cold storage.

Thermal inactivation of polyphenoloxidase and peroxidase in Jubileu clingstone peach and yeast isolated from its spoiled puree

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Andréa Menezes Lopes

2014-03-01

Full Text Available The thermal inactivation of yeast isolated from spoiled Jubileu peach puree and that of polyphenoloxidase (PPO and peroxidase (POD in cv. Jubileu, which is widely cultivated in southern Rio Grande do Sul state, Brazil, were studied. PPO and POD were extracted using the protein powder method and submitted to partial purification by precipitation followed by dialysis. The enzymatic activity was determined measuring the increase in absorbance at 420 nm for PPO and 470 nm for POD. The yeast used in this investigation was isolated from spoiled Jubileu peach puree at 22 °Brix, with total initial microbial count of 22 × 10² UFCmL- 1. Stock cultures were maintained on potato dextrose agar (PDA slants at 4 °C and pH 5 for later use for microbial growth. In all cases, kinetic analysis of the results suggests that the thermal inactivation was well described by a first-order kinetic model, and the temperature dependence was significantly represented by the Arrhenius law. Both enzymes were affected by heat denaturation, and PPO was more thermostable. PPO was also more thermosTable than the yeast isolated from peach puree. The D60-values were 1.53 and 1.87 min for PPO and yeast isolated from spoiled Jubileu peach puree, respectively.

Inactivation of contaminated fungi and antioxidant effects of peach (Prunus persica L. Batsch cv Dangeumdo) by 0.5-2 kGy gamma irradiation

Energy Technology Data Exchange (ETDEWEB)

Kim, Kyoung-Hee; Kim, Mi-Seon [Department of Food and Nutrition, Chungnam National University, Daejeon 305-764 (Korea, Republic of); Kim, Hong-Gi [Department of Applied Biology, Chungnam National University, Daejeon 305-764 (Korea, Republic of); Yook, Hong-Sun, E-mail: yhsuny@naver.co [Department of Food and Nutrition, Chungnam National University, Daejeon 305-764 (Korea, Republic of)

2010-04-15

The effect of gamma irradiation (0.5-2 kGy) on the physicochemical properties of peaches was investigated during a 6-day storage at 20+-3 deg. C. Gamma irradiation is able to inactivate the four pathogens, namely Botrytis cinerea, Penicillium expansum, Rhizopus stolonifer var. stolonifer and Monilinia fructicola in peaches. Hardness significantly decreased with the increment of irradiation dose level whereas soluble solid and total polyphenol contents increased with increment of irradiation dose level. 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity of the irradiated peach was higher than that of control, and its activity increased with increment of irradiation dose level. These results suggest that gamma irradiation of peaches improved antioxidant activity, but dramatically affects the hardness throughout the entire storage time.

Inactivation of contaminated fungi and antioxidant effects of peach (Prunus persica L. Batsch cv Dangeumdo) by 0.5-2 kGy gamma irradiation

International Nuclear Information System (INIS)

Kim, Kyoung-Hee; Kim, Mi-Seon; Kim, Hong-Gi; Yook, Hong-Sun

2010-01-01

The effect of gamma irradiation (0.5-2 kGy) on the physicochemical properties of peaches was investigated during a 6-day storage at 20±3 deg. C. Gamma irradiation is able to inactivate the four pathogens, namely Botrytis cinerea, Penicillium expansum, Rhizopus stolonifer var. stolonifer and Monilinia fructicola in peaches. Hardness significantly decreased with the increment of irradiation dose level whereas soluble solid and total polyphenol contents increased with increment of irradiation dose level. 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity of the irradiated peach was higher than that of control, and its activity increased with increment of irradiation dose level. These results suggest that gamma irradiation of peaches improved antioxidant activity, but dramatically affects the hardness throughout the entire storage time.

Co-combustion of peach and apricot stone with coal in a bubbling fluidized bed

Energy Technology Data Exchange (ETDEWEB)

Atimtay, Aysel T.; Kaynak, Burcak [Department of Environmental Engineering, Middle East Technical University, Ankara 06531 (Turkey)

2008-02-15

In this study a bubbling fluidized bed combustor (BFBC) having an inside diameter of 102 mm and a height of 900 mm was used to investigate the co-combustion characteristics of peach and apricot stones produced as a waste from the fruit juice industry with coal. A lignite coal was used for co-combustion. On-line concentrations of O{sub 2}, CO, CO{sub 2}, SO{sub 2}, NO{sub X} and total hydrocarbons (C{sub m}H{sub n}) were measured in the flue gas during combustion experiments. Variations of emissions of various pollutants were studied by changing the operating parameters (excess air ratio, fluidization velocity, and fuel feed rate). Temperature distribution along the bed was measured with thermocouples. For co-combustion of apricot and peach fruit stones with a lignite coal, various ratios of biomass to coal ranging from 0 to 100 wt.% were tested. For the peach stone co-combustion tests, efficiencies are about 98% and for the apricot stone co-combustion tests, efficiencies ranged between 94.7% and 96.9% for 25%, 50% and 75% of apricot stone in the fuel mixture. The results of this study have shown that as the biomass ratio in the fuel mixture increases, the combustion takes place at the upper regions of the main column. This causes higher temperatures in the freeboard than the bed. Also the CO and hydrocarbon (C{sub m}H{sub n}) emissions increase as the biomass percentage increases in the fuel mixture. This causes decrease in the combustion efficiency. These results suggest that peach and apricot stones are potential fuels that can be utilized for clean energy production in small-scale fruit juice industries by using BFBC. The percentage of peach stones or apricot stones in the fuel mixture is suggested to be below 50 wt.% in order to obtain the emission limits of EU. During the design of the BFBC, one has to be careful about the volatile matter (VM) content of the biomass. For the complete combustion of the VM, longer freeboard or secondary air addition should be

Comparative Study between Ethanolic and β-Cyclodextrin Assisted Extraction of Polyphenols from Peach Pomace

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Nada El Darra

2018-01-01

Full Text Available Peach byproducts are often regarded as food waste despite their high content in health-promoting components. Amongst the latter, polyphenols are bioactive molecules with significant health benefits. The present study investigated an eco-friendly and cost-effective method using a GRAS food additive, β-cyclodextrin (β-CD, for the recovery of polyphenols from peach pomace. β-CD assisted extraction of polyphenols was compared to that of conventional solvent (ethanol extraction at the same concentrations (10 mg/mL, 20 mg/mL, 30 mg/mL, 40 mg/mL, and 50 mg/mL in terms of quality (antiradical activity and quantity. The extract obtained by 50 mg/mL β-CD assisted extraction showed the highest polyphenol (0.72 mg GAE/g DM and flavonoid (0.35 mg catechin/g of DM concentrations as maximal antiradical activity (6.82% and a noted antibacterial activity. Our results showed the competitiveness of β-CD assisted extraction to recover a high quantity and quality of polyphenols from peach pomace suggesting β-CD as a green alternative method for phenolic extraction.

Evaluation of fruit quality, bioactive compounds and total antioxidant activity of flat peach cultivars.

Science.gov (United States)

Di Vaio, Claudio; Marallo, Nadia; Graziani, Giulia; Ritieni, Alberto; Di Matteo, Antonio

2015-08-15

Fruit quality traits (fresh weight, dry weight, soluble solids content, titratable acidity and firmness) as well as the content of bioactive compounds (phenolic compounds) and total antioxidant activity were evaluated in four commercial cultivars of peach (Greta, Ufo 4, Rome Star and Ufo 6) and four of nectarine (Neve, Planet 1, Maria Carla and Mesembrina) differing in fruit shape (standard or flat) and flesh colour (white or yellow), important cultivars of the Italian and foreign market. The higher fruit organoleptic quality and nutritional profile of flat peach and nectarine cultivars make them candidates for exploiting new market opportunities and the chance to improve profits of farmers. The results showed that assayed quality parameters differed greatly among cultivars. In particular, flesh color and fruit shape accounted for most of the variation in traits underlying organoleptic and nutritional quality. Overall data suggested that the flat white-fleshed nectarine Planet 1, the yellow-fleshed nectarine Mesembrina and the yellow-fleshed peach Ufo 6, because of their profiles in terms of soluble solids content, titratable acidity and bioactive compounds, have the greatest potential to meet current consumer requirements. © 2014 Society of Chemical Industry.

Analysis of containment venting at the Peach Bottom atomic power station

International Nuclear Information System (INIS)

Hanson, D.J.; Blackman, H.S.; Nelson, W.R.; Wright, R.E.; Leonard, M.T.; DiSalvo, R.

1988-01-01

An analysis of the extent to which containment venting would be effective in preventing or mitigating the consequences of severe accidents has been completed for the Peach Bottom Units 2 and 3 (BWR-4s with Mark I containments). The analysis indicates that the effectiveness of venting in preventing containment overpressurization highly depends on the sequence of the severe accident. Containment venting can be effective for several classes of sequences, including transients with failure of long-term decay heat removal and loss-of-coolant accidents with breaks inside the containment. However, based on draft procedures and equipment in place at the time of the evaluation, containment venting has limited potential for further reducing the risk associated with three severe accident sequences currently identified as important risk contributors at Peach Bottom. Means of improving the potential for risk reduction is identified, but their influence on risk is not analyzed. (orig./HP)

Analysis of containment venting at the Peach Bottom Atomic Power Station

International Nuclear Information System (INIS)

Hanson, D.J.; Blackman, H.S.; Nelson, W.R.; Wright, R.E.; Leonard, M.T.; DiSalvo, R.

1986-01-01

An analysis of the extent to which containment venting would be effective in preventing or mitigating the consequences of severe accidents has been completed for the Peach Bottom Atomic Power Station, Units 2 and 3 (BWR-4s with Mark I containments). The analysis indicates that the effectiveness of venting in preventing containment overpressurization highly depends on the sequence of the severe accident. Containment venting can be effective for several classes of sequences, including transients with failure of long-term decay heat removal and loss-of-coolant accidents with breaks inside the containment. However, based on draft procedures and equipment in place at the time of the evaluation, containment venting has limited potential for further reducing the risk associated with three severe accident sequences currently identified as important risk contributors at Peach Bottom. Means of improving the potential for risk reduction is identified, but their influence on risk is not analyzed

Nutritional status, yield and composition of peach fruit subjected to the application of organic compost

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George Wellington Bastos de Melo

2016-01-01

Full Text Available The purpose of this study was to evaluate the nutritional state, yield and composition of peaches on peach trees subjected to the application of organic compost to the soil. This experiment was conducted during the 2008 and 2009 cropping season in an orchard containing Chimarrita cultivars grafted onto Capdeboscq rootstocks and Haplumbrept soils in the municipality of Farroupilha (RS, Brazil. The treatments included 0, 9, 18, 36, 72 and 144 liters of organic compost per plant-1 year-1. The total nutrient contents in the leaves, yield components, yields per plant and hectare and compositions of the fruits were evaluated in 2008 and 2009 soon after harvest and after 30 days of storage. The application of organic compost to the soil increased the yield components and the yields per plant and hectare in the two treatments with the highest compost additions, which indicated that the addition of 72 L of compost per plant-1 is ideal economically. The organic compost had little effect on the composition of the peach fruit after harvest and after 30 days of storage.

Effects of ambient temperature on egg and larval development of the invasive emerald ash borer (Coleoptera: Buprestidae): implications for laboratory rearing.

Science.gov (United States)

Duan, Jian J; Watt, Tim; Taylor, Phil; Larson, Kristi; Lelito, Jonathan P

2013-10-01

The emerald ash borer, Agrilus planipennis Fairmaire, an invasive beetle from Asia causing large scale ash (Fraxinus) mortality in North America, has been extremely difficult to rear in the laboratory because of its long life cycle and cryptic nature of immature stages. This lack of effective laboratory-rearing methods has not only hindered research into its biology and ecology, but also mass production of natural enemies for biological control of this invasive pest. Using sticks from the alternate host plant, Fraxinus uhdei (Wenzig) Lingelsh, we characterized the stage-specific development time and growth rate of both emerald ash borer eggs and larvae at different constant temperatures (12-35 degrees C) for the purpose of developing effective laboratory-rearing methods. Results from our study showed that the median time for egg hatching decreased from 20 d at 20 degrees C to 7 d at 35 degrees C, while no emerald ash borer eggs hatched at 12 degrees C. The developmental time for 50% of emerald ash borer larvae advancing to third, fourth, and J-larval stages at 20 degrees C were 8.3, 9.1, and 12.3 wk, respectively, approximately two times longer than at 30 degrees C for the corresponding instars or stages. In contrast to 30 degrees C, however, the development times of emerald ash borer larvae advancing to later instars (from oviposition) were significantly increased at 35 degrees C, indicating adverse effects of this high temperature. The optimal range of ambient temperature to rear emerald ash borer larvae should be between 25-30 degrees C; however, faster rate of egg and larval development should be expected as temperature increases within this range.

Antifeedant Diterpenoids against Tribolium castaneum from the Stems and Twigs of Ceriops tagal (Rhizophoraceae

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Zhi Wei Deng

2011-07-01

Full Text Available The screening of several Chinese mangrove plants for insecticidal principles showed that ethanol extract of Ceriops tagal stems and twigs possessed significant feeding deterrent activity against the red flour beetle, Tribolium castaneum (Family: Rhizophoraceae. From the ethanol extract, three feeding deterrent diterpenoids were isolated by bioassay-guided fractionation. The compounds were identified as tagalsin A, B, and H on the basis of their phytochemical and spectral data. Tagalsin A, B, and H exhibited strong feeding deterrent activity against T. castaneum adults with EC50 values of 375.3 ppm, 277.3 ppm, and 285.45 ppm, respectively.

GC×GC-TOFMS Analysis of Essential Oils Composition from Leaves, Twigs and Seeds of Cinnamomum camphora L. Presl and Their Insecticidal and Repellent Activities.

Science.gov (United States)

Jiang, Hao; Wang, Jin; Song, Li; Cao, Xianshuang; Yao, Xi; Tang, Feng; Yue, Yongde

2016-03-28

Interest in essential oils with pesticidal activity against insects and pests is growing. In this study, essential oils from different parts (leaves, twigs and seeds) of Cinnamomum camphora L. Presl were investigated for their chemical composition, and insecticidal and repellent activities against the cotton aphid. The essential oils, obtained by hydrodistillation, were analyzed by GC×GC-TOFMS. A total of 96 components were identified in the essential oils and the main constituents found in the leaves and twigs were camphor, eucalyptol, linalool and 3,7-dimethyl-1,3,7-octatriene. The major components found in the seeds were eucalyptol (20.90%), methyleugenol (19.98%), linalool (14.66%) and camphor (5.5%). In the contact toxicity assay, the three essential oils of leaves, twigs and seeds exhibited a strong insecticidal activity against cotton aphids with LC50 values of 245.79, 274.99 and 146.78 mg/L (after 48 h of treatment), respectively. In the repellent assay, the highest repellent rate (89.86%) was found in the seed essential oil at the concentration of 20 μL/mL after 24 h of treatment. Linalool was found to be a significant contributor to the insecticidal and repellent activities. The results indicate that the essential oils of C. camphora might have the potential to be developed into a natural insecticide or repellent for controlling cotton aphids.

Interspecific variation in resistance of Asian, European, and North American birches (Betula spp.) to bronze birch borer (Coleoptera: Buprestidae).

Science.gov (United States)

Nielsen, David G; Muilenburg, Vanessa L; Herms, Daniel A

2011-06-01

Bronze birch borer (Agrilus anxius Gory) is the key pest of birches (Betula spp.) in North America, several of which have been recommended for ornamental landscapes based on anecdotal reports of borer resistance that had not been confirmed experimentally. In a 20-yr common garden experiment initiated in 1979 in Ohio, North American birch species, including paper birch (Betula papyrifera Marshall), 'Whitespire' gray birch (Betula populifolia Marshall), and river birch (Betula nigra L.), were much more resistant to bronze birch borer than species indigenous to Europe and Asia, including European white birch (Betula pendula Roth), downy birch (Betula pubescens Ehrh.), monarch birch (Betula maximowicziana Regel), and Szechuan white birch (Betula szechuanica Jansson). Within 8 yr of planting, every European white, downy, and Szechuan birch had been colonized and killed, although 100% of monarch birch had been colonized and 88% of these plants were killed after nine years. Conversely, 97% of river birch, 76% of paper birch, and 73% Whitespire gray birch were alive 20 yr after planting, and river birch showed no evidence of colonization. This pattern is consistent with biogeographic theory of plant defense: North American birch species that share a coevolutionary history with bronze birch borer were much more resistant than naïve hosts endemic to Europe and Asia, possibly by virtue of evolution of targeted defenses. This information suggests that if bronze birch borer were introduced to Europe or Asia, it could threaten its hosts there on a continental scale. This study also exposed limitations of anecdotal observation as evidence of host plant resistance.

Genomic characterization of putative allergen genes in peach/almond and their synteny with apple

Science.gov (United States)

Chen, Lin; Zhang, Shuiming; Illa, Eudald; Song, Lijuan; Wu, Shandong; Howad, Werner; Arús, Pere; Weg, Eric van de; Chen, Kunsong; Gao, Zhongshan

2008-01-01

Background Fruits from several species of the Rosaceae family are reported to cause allergic reactions in certain populations. The allergens identified belong to mainly four protein families: pathogenesis related 10 proteins, thaumatin-like proteins, lipid transfer proteins and profilins. These families of putative allergen genes in apple (Mal d 1 to 4) have been mapped on linkage maps and subsequent genetic study on allelic diversity and hypoallergenic traits has been carried out recently. In peach (Prunus persica), these allergen gene families are denoted as Pru p 1 to 4 and for almond (Prunus dulcis)Pru du 1 to 4. Genetic analysis using current molecular tools may be helpful to establish the cause of allergenicity differences observed among different peach cultivars. This study was to characterize putative peach allergen genes for their genomic sequences and linkage map positions, and to compare them with previously characterized homologous genes in apple (Malus domestica). Results Eight Pru p/du 1 genes were identified, four of which were new. All the Pru p/du 1 genes were mapped in a single bin on the top of linkage group 1 (G1). Five Pru p/du 2 genes were mapped on four different linkage groups, two very similar Pru p/du 2.01 genes (A and B) were on G3, Pru p/du 2.02 on G7,Pru p/du 2.03 on G8 and Pru p/du 2.04 on G1. There were differences in the intron and exon structure in these Pru p/du 2 genes and in their amino acid composition. Three Pru p/du 3 genes (3.01–3.03) containing an intron and a mini exon of 10 nt were mapped in a cluster on G6. Two Pru p/du 4 genes (Pru p/du 4.01 and 4.02) were located on G1 and G7, respectively. The Pru p/du 1 cluster on G1 aligned to the Mal d 1 clusters on LG16; Pru p/du 2.01A and B on G3 to Mal d 2.01A and B on LG9; the Pru p/du 3 cluster on G6 to Mal d 3.01 on LG12; Pru p/du 4.01 on G1 to Mal d 4.03 on LG2; and Pru p/du 4.02 on G7 to Mal d 4.02 on LG2. Conclusion A total of 18 putative peach/almond allergen genes have

Genomic characterization of putative allergen genes in peach/almond and their synteny with apple

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Weg Eric

2008-11-01

Full Text Available Abstract Background Fruits from several species of the Rosaceae family are reported to cause allergic reactions in certain populations. The allergens identified belong to mainly four protein families: pathogenesis related 10 proteins, thaumatin-like proteins, lipid transfer proteins and profilins. These families of putative allergen genes in apple (Mal d 1 to 4 have been mapped on linkage maps and subsequent genetic study on allelic diversity and hypoallergenic traits has been carried out recently. In peach (Prunus persica, these allergen gene families are denoted as Pru p 1 to 4 and for almond (Prunus dulcisPru du 1 to 4. Genetic analysis using current molecular tools may be helpful to establish the cause of allergenicity differences observed among different peach cultivars. This study was to characterize putative peach allergen genes for their genomic sequences and linkage map positions, and to compare them with previously characterized homologous genes in apple (Malus domestica. Results Eight Pru p/du 1 genes were identified, four of which were new. All the Pru p/du 1 genes were mapped in a single bin on the top of linkage group 1 (G1. Five Pru p/du 2 genes were mapped on four different linkage groups, two very similar Pru p/du 2.01 genes (A and B were on G3, Pru p/du 2.02 on G7,Pru p/du 2.03 on G8 and Pru p/du 2.04 on G1. There were differences in the intron and exon structure in these Pru p/du 2 genes and in their amino acid composition. Three Pru p/du 3 genes (3.01–3.03 containing an intron and a mini exon of 10 nt were mapped in a cluster on G6. Two Pru p/du 4 genes (Pru p/du 4.01 and 4.02 were located on G1 and G7, respectively. The Pru p/du 1 cluster on G1 aligned to the Mal d 1 clusters on LG16; Pru p/du 2.01A and B on G3 to Mal d 2.01A and B on LG9; the Pru p/du 3 cluster on G6 to Mal d 3.01 on LG12; Pru p/du 4.01 on G1 to Mal d 4.03 on LG2; and Pru p/du 4.02 on G7 to Mal d 4.02 on LG2. Conclusion A total of 18 putative peach

Activated carbon from peach stones using phosphoric acid activation at medium temperatures.

Science.gov (United States)

Kim, Dong-Su

2004-01-01

In the present study, the activation features of phosphoric acid have been investigated using waste peach stones as the raw material in the production of granular activated carbon. Thermogravimetry/differential thermal analysis was conducted to characterize the thermal behavior of peach stone and titration method was used to evaluate the adsorption capacity of the produced activated carbon. It was observed that the iodine value of the activated carbon increased with activation temperature. However, temperatures higher than 500 degrees C caused a thermal destruction, which resulted in the decrease of the adsorption capacity. Activation longer than 1.5 h at 500 degrees C resulted in thermal degradation of the porous structure of the activated carbon. The adsorption capacity was enhanced with increasing of amounts of phosphoric acid, however, excessive phosphoric acid caused a decrease in the iodine value. In addition, it was found that the carbon yields generally decreased with activation temperature and activation time. Scanning electron microscopy analysis was conducted to observe the changes in the poros structure of the activated carbon produced in different temperatures. Activation of carbon by phosphoric acid was found to be superior to that by CaCl2 and gas activation. The activated carbon produced from peach stone was applied as an adsorbent in the treatment of synthesized wastewater containing cadmium ion and its adsorption capacity was found to be as good as that of the commercial one.

76 FR 31295 - Nectarines and Peaches Grown in California; Notice of Withdrawal

Science.gov (United States)

2011-05-31

... forms to collect information related to the Federal marketing orders for nectarines and peaches grown in... FURTHER INFORMATION CONTACT: Andrew Hatch, Supervisory Marketing Specialist, Marketing Order... Email: [email protected] . SUPPLEMENTARY INFORMATION: Marketing Order Nos. 916 and 917...

Effects of exogenous salicylic acid on physiological traits and CBF gene expression in peach floral organs under freezing stress

Directory of Open Access Journals (Sweden)

Zhang Binbin

2017-01-01

Full Text Available To elucidate the effects of exogenous salicylic acid (SA treatment on the cold resistance of peach flower, the floral organs of two peach cultivars were treated with 20 mg/L SA and stored at 0°C for observation and sample collection. Water application was the control. After a treatment period, the anther relative water content of the control and SA-treated flowers decreased. The extent of the reduction was greater in the control, suggesting that the SA treatment significantly helped to maintain the anther water content of peach. Analysis of the stigma relative electric conductivity revealed that the SA treatment prevented membrane injury during the low temperature treatment. Additionally, we measured CBF gene expression at low temperature in the petal, stigma and ovary. The expression was markedly upregulated in the cold-treated floral organs. CBF gene expression after SA treatment was higher than in the control when cold conditions continued. These results suggest that the effects of SA on ameliorating the freezing injury to peach floral organs and on enhancing cold tolerance may be associated with the induction of CBF gene.

Hypersensitivity to Tomato (Lycopersicon esculentum) in Peach-Allergic Patients: rPrup 3 and rPrup 1 Are Predictive of Symptom Severity.

Science.gov (United States)

Mascheri, A; Farioli, L; Pravettoni, V; Piantanida, M; Stafylaraki, C; Scibilia, J; Mirone, C; Preziosi, D; Nichelatti, M; Pastorello, E A

2015-01-01

Background: The role of allergens in the severity of tomato allergy symptoms has not yet been studied. To evaluate the relationship between severe allergic reactions to peach and tomato and between tomato allergy symptoms and the pattern of IgE positivity for rPru p 1, rPru p 3, rPru p 4, rBetv 1, rBetv 2, rBetv4, rPhl p 1, and rPhl p 12 in order to identify the role of recombinant allergens in the severity of reactions to tomato. We studied peach-allergic patients with clinical reactions to tomato by performing an open food challenge, skin prick test, and determination of serum specific IgE to tomato and to recombinant peach, birch, and grass allergens. Statistical analysis was carried out to evaluate the relationship between the severity of tomato symptoms and IgE positivity to the different allergens and to peach-induced symptoms. We found a significant association between severe reactions to tomato and severe reactions to peach (P = .01 7) and levels of IgE to rPru p3 (P = .029) and between mild tomato allergy symptoms and levels of IgE to rPru p1 (P = .047), anti-rBetv 1 (P = .0414), anti-rBetv 2 (P = .0457), and Phleum pratense (P = .0022). We observed a significant relationship between peach and symptoms of tomato allergy. IgE positivity for rPru p3 seems to be a surrogate biochemical marker for severe tomato allergy, whereas the presence of anti-rPru p 1 IgE may be an indicator of mild tomato allergy.

Undecomposed Twigs in the Leaf Litter as Nest-Building Resources for Ants (Hymenoptera: Formicidae in Areas of the Atlantic Forest in the Southeastern Region of Brazil

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Tae Tanaami Fernandes

2012-01-01

Full Text Available In tropical forests, the leaf-litter stratum exhibits one of the greatest abundances of ant species. This diversity is associated with the variety of available locations for nest building. Ant nests can be found in various microhabitats, including tree trunks and fallen twigs in different stages of decomposition. In this study, we aimed to investigate undecomposed twigs as nest-building resources in the leaf litter of dense ombrophilous forest areas in the southeastern region of Brazil. Demographic data concerning the ant colonies, the physical characteristics of the nests, and the population and structural of the forest were observed. Collections were performed manually over four months in closed canopy locations that did not have trails or flooded areas. A total of 294 nests were collected, and 34 ant species were recorded. Pheidole, Camponotus, and Hypoponera were the richest genera observed; these genera were also among the most populous and exhibited the greatest abundance of nests. We found no association between population size and nest diameter. Only tree cover influenced the nest abundance and species richness. Our data indicate that undecomposed twigs may be part of the life cycle of many species and are important for maintaining ant diversity in the leaf litter.

Supercritical fluid extraction of peach (Prunus persica) almond oil: process yield and extract composition.

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Mezzomo, Natália; Mileo, Bruna R; Friedrich, Maria T; Martínez, Julian; Ferreira, Sandra R S

2010-07-01

Peach kernels are industrial residues from the peach processing, contain oil with important therapeutic properties and attractive nutritional aspects because of the high concentration of oleic and linoleic acids. The extraction method used to obtain natural compounds from raw matter is critical for product quality definition. Thus, the aim of this work was to compare peach almond extraction yields obtained by different procedures: soxhlet extractions (Sox) with different solvents; hydrodistillation (HD); ethanolic maceration (Mac) followed by fractionation with various solvents, and supercritical fluid extraction (SFE) at 30, 40 and 50 degrees C and at 100, 200 and 300bar, performed with pure CO(2) and with a co-solvent. The extracts were evaluated with respect to fatty acid composition (FAC), fractionated chemical profile (FCP) and total phenolic content (TPC). The Sox total yields were generally higher than those obtained by SFE. The crossover pressure for SFE was between 260 and 280bar. The FAC results show oleic and linoleic acids as main components, especially for Sox and SFE extracts. The FCP for samples obtained by Sox and Mac indicated the presence of benzaldehyde and benzyl alcohol, components responsible for almond flavor and with important industrial uses, whereas the SFE extracts present a high content of a possible flavonoid. The higher TPC values were obtained by Sox and Mac with ethanol. In general, the maximum pressure in SFE produced the highest yield, TPC and oleic acid content. The use of ethanol at 5% as co-solvent in SFE did not result in a significant effect on any evaluated parameter. The production of peach almond oil through all techniques is substantially adequate and SFE presented advantages, with respect to the quality of the extracts due to the high oleic acid content, as presented by some Sox samples. Copyright (c) 2010 Elsevier Ltd. All rights reserved.

Study on the growth and development of brinjal shoot and fruit borer ...

African Journals Online (AJOL)

Jane

2011-09-28

Sep 28, 2011 ... 2Department of Crop Science, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. ... various aspects of the insect's life and activity with a view to evolving .... of brinjal shoot and fruit borer is still a difficult job. Many.

Biotechnology for cocoa pod borer resistance in cocoa

OpenAIRE

Chaidamsari, T.

2005-01-01

The cocoa tree ( Theobroma cacao L.) produces the beans that are the source of cacao, the basis for chocolate production, and an important commodity crop in South America, West Africa, and Southeast Asia.Cocoa Pod Borer (CPB,( Conopomorpha cramerella)has been the single most important limiting factor for cacao production in Southeast Asia.So far, there has been no single cost effective and environmentally safe way to control this pest.This thesis describes the first steps in a biotechnologica...

Cost of potential emerald ash borer damage in U.S

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Kent F. Kovacs; Robert G. Haight; Andrew M. Liebhold; Deborah G. McCullough; Rodrigo J. Mercader; Nathan W. Siegert

2010-01-01

Emerald ash borer (EAB; Agrilus planipennis Fairmaire), a phloem-feeding beetle native to Asia, was discovered near Detroit, MI, and Windsor, ON, in 2002. As of March 2009, isolated populations of EAB have been detected in nine additional states and Quebec. EAB is a highly invasive forest pest that has the potential to spread and kill native ash...

The coffee berry borer, Hypothenemus hampei: how many instars are there?

Science.gov (United States)

After more than a century since the description of the coffee berry borer, Hypothenemus hampei (Ferrari), and dozens of scientific articles on the basic biology of the insect, there is still debate on the number of female larval instars. This paper analyzes the metamorphosis of H. hampei females thr...

Altitudinal variation in growth, bud break and susceptibility to balsam twig aphid damage of balsam fir from 6 Vermont seed sources

Science.gov (United States)

Ronald C. Wilkinson; Paul G. Schaberg

1992-01-01

Differences in 10-year heights, 4-year growth from 1987 through 1990, relative timing of budbreak and damage by the balsam twig aphid (Mindarus abietinus Koch.) among balsam fir (Abies balsamea (L.) Mill.) from 6 Vermont seed sources originating from different elevations were examined. Height differences among seed sources were...

Development of biocontrol agents from food microbial isolates for controlling post-harvest peach brown rot caused by Monilinia fructicola.

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Zhou, Ting; Schneider, Karin E; Li, Xiu-Zhen

2008-08-15

An unconventional strategy of screening food microbes for biocontrol activity was used to develop biocontrol agents for controlling post-harvest peach brown rot caused by Monilinia fructicola. Forty-four microbial isolates were first screened for their biocontrol activity on apple fruit. Compared with the pathogen-only check, seven of the 44 isolates reduced brown rot incidence by >50%, including four bacteria: Bacillus sp. C06, Lactobacillus sp. C03-b and Bacillus sp. T03-c, Lactobacillus sp. P02 and three yeasts: Saccharomyces delbrueckii A50, S. cerevisiae YE-5 and S. cerevisiae A41. Eight microbial isolates were selected for testing on peaches by wound co-inoculation with mixtures of individual microbial cultures and conidial suspension of M. fructicola. Only two of them showed significant biocontrol activity after five days of incubation at 22 degrees C. Bacillus sp. C06 suppressed brown rot incidence by 92% and reduced lesion diameter by 88% compared to the pathogen-only check. Bacillus sp.T03-c reduced incidence and lesion diameter by 40% and 62%, respectively. The two isolates were compared with Pseudomonas syringae MA-4, a biocontrol agent for post-harvest peach diseases, by immersing peaches in an aliquot containing individual microbial isolates and the pathogen conidia. Treatments with isolates MA-4, C06 and T03-c significantly controlled brown rot by 91, 100, and 100% respectively. However, only isolates MA-4 and C06 significantly reduced brown rot by 80% and 15%, respectively when bacterial cells alone were applied. On naturally infected peaches, both the bacterial culture and its cell-free filtrate of the isolate C06 significantly controlled peach decay resulting in 77 and 90% reduction, respectively, whereas the treatment using only the bacterial cells generally had no effect. Isolate C06 is a single colony isolate obtained from a mesophilic cheese starter, and has been identified belonging to Bacillus amyloliquefaciens. The results have clearly

7 CFR 319.56-23 - Apricots, nectarines, peaches, plumcot, and plums from Chile.

Science.gov (United States)

2010-01-01

... organization of Chile (Servicio Agricola y Ganadero, referred to in this section as SAG) or a private export...) Responsibilities of Servicio Agricola y Ganadero. SAG will ensure that: (1) Apricots, nectarines, peaches, plumcot...

Pest Management Strategies Against the Coffee Berry Borer (Coleoptera: Curculionidae: Scolytinae).

Science.gov (United States)

Infante, Francisco

2018-03-22

Coffee ( Coffea arabica and C. canephora) is one of the most widely traded agricultural commodities and the main cash crop in ∼80 tropical countries. Among the factors that limit coffee production, the coffee berry borer, Hypothenemus hampei (Ferrari) has been considered the main insect pest, causing losses of over U.S. $500 million dollars annually. Control of this pest has been hindered by two main factors: the cryptic nature of the insect (i.e., protected inside the coffee berry) and the availability of coffee berries in the field allowing the survival of the pest from one generation to the next. Coffee berry borer control has primarily been based on the use of synthetic insecticides. Management strategies have focused on the use of African parasitoids ( Cephalonomia stephanoderis, Prorops nasuta, and Phymastichus coffea), fungal entomopathogens ( Beauveria bassiana), and insect traps. These approaches have had mixed results. Recent work on the basic biology of the insect has provided novel insights that might be useful in developing novel pest management strategies. For example, the discovery of symbiotic bacteria responsible for caffeine breakdown as part of the coffee berry borer microbiome opens new possibilities for pest management via the disruption of these bacteria. Some chemicals with repellent propieties have been identified, and these have a high potential for field implementation. Finally, the publication of the CBB genome has provided insights on the biology of the insect that will help us to understand why it has been so successful at exploiting the coffee plant. Here I discuss the tools we now have against the CBB and likely control strategies that may be useful in the near future.

Study on The Content of Pb in Twigs And Leaves of Kangkung (Ipomoea reptans Poir Boiled With The Addition of NaCl And Acetic Acid

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Poppy Hartatie Hardjo

2004-12-01

Full Text Available Kangkung is a kind of favorable vegetables that used to grow near a river, and is cultivated and watered with water from the river. If the river is polluted by heavy metals, there is a risk that the plant is contaminated too. A study on the content of Pb in kangkung planted in Pb contaminated media has been conducted, and it was proven that Pb was found in the plant. Land kangkung (Ipomoea reptans was used as sample, and was planted in hydrophonic media, and watered with Multigrow Complete Plant Food (2000 mg/L and Pb solution (2 mg/L twice a day. Samples were taken based on the age of 54 days, then the twigs and leaves were boiled in different ways: I. Boiled with no addition, II. Boiled with addition of NaCl , and III. Boiled with addition of acetic acid. IV. Unboiled sample as the control. Inductively Coupled Plasma Spectrometer (ICPS Fison 3410+ was used to measure the Pb content. It was shown that boiling the kangkung reduced the Pb content in the leaves as well as in the twigs; however, the acetic acid addition showed the least effect. In the leaves the three different ways of boiling did not show significant different, while in twigs the different was significant.

Coffee berry borer in conilon coffee in the Brazilian Cerrado: an ancient pest in a new environment.

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Oliveira, C M; Santos, M J; Amabile, R F; Frizzas, M R; Bartholo, G F

2018-02-01

The aim of this study was to verify the occurrence of the coffee berry borer, Hypothenemus hampei (Ferrari), and to evaluate the population fluctuation of the pest in the Brazilian Cerrado (Federal District). The study was conducted, between November 2014 and October 2015, at Embrapa Cerrados (Planaltina/DF, Brazil) in an irrigated conilon coffee production area. In November 2014, 120 samples (ten berries/sample) were collected from berries that had fallen on the ground from the previous harvest. Between November 2014 and October 2015, insects were collected weekly, using traps (polyethylene terephthalate bottles) baited with ethyl alcohol (98 GL), ethyl alcohol (98 GL) with coffee powder, or molasses. Between January and July 2015, samples were collected fortnightly from 92 plants (12 berries per plant). All samples were evaluated for the presence of adult coffee berry borers. Samples from the previous harvest had an attack incidence of 72.4%. The baited traps captured 4062 H. hampei adults, and showed no statistical difference in capture efficiency among the baits. Pest population peaked in the dry season, with the largest percentage of captured adults occurring in July (31.0%). An average of 18.6% of the collected berries was attacked by the borer and the highest percentage incidence was recorded in July (33.2%). Our results suggest that the coffee berry borer, if not properly managed, could constitute a limiting factor for conilon coffee production in the Brazilian Cerrado.

Impact evaluation of integrated food-bioenergy systems: A comparative LCA of peach nectar

International Nuclear Information System (INIS)

De Menna, Fabio; Vittuari, Matteo; Molari, Giovanni

2015-01-01

Processed food products present high energy intensity, along with a large amount of food losses and waste. The recovery of residual biomass as integrated renewable energy source could represent an interesting option for the substitution of fossil energy, contributing to the transition of agro-food sector towards a low-carbon economy. Two scenarios were compared, in order to evaluate the impacts of a fossil fuel-based food chain and the potential benefits of the integration of bioenergy production, using peach nectar as case study. In the first scenario, peach nectar is produced, distributed and consumed using fossil energy, while residuals are wasted. In the second scenario, byproducts from the nectar chain are used to produce bioenergy from combustion or anaerobic digestion, which is then consumed to substitute electricity and heat. A comparative life cycle assessment (LCA) based on the same functional unit was performed. Main results show that, in the conventional scenario, most of the damage derives from land use, especially for sugar and glucose production, from the fossil energy consumption of about 15 MJ l −1 , and the related greenhouse gas (GHG) emissions of 0.91 kg CO 2  eq l −1 . Food waste leads to a loss of about 20 kcal l −1 . Bioenergy integration would allow a 13–15% damage reduction, mainly due to the substitution of indirect energy consumption. The effects on human health and ecosystem quality are limited. - Highlights: • Up to 15 MJ l −1 of fossil energy are needed to produce 2.7 MJ of peach nectar. • About 20 out of 648 kcal l −1 of peach and nectar are wasted along the supply chain. • Added ingredients (sugar and glucose) cause a large share of land use impact. • Bioenergy from waste reduces up to 37% of non-renewable energy consumption

Endogenous hormones response to cytokinins with regard to organogenesis in explants of peach (Prunus persica L. Batsch) cultivars and rootstocks (P. persica × Prunus dulcis).

Science.gov (United States)

Pérez-Jiménez, Margarita; Cantero-Navarro, Elena; Pérez-Alfocea, Francisco; Cos-Terrer, José

2014-11-01

Organogenesis in peach (Prunus persica L. Batsch) and peach rootstocks (P. persica × Prunus dulcis) has been achieved and the action of the regeneration medium on 7 phytohormones, zeatin (Z), zeatin riboside (ZR), indole-3-acetic acid (IAA), abscisic acid (ABA), ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), salicylic acid (SA), and jasmonic acid (JA), has been studied using High performance liquid chromatography - mass spectrometry (HPLC-MS/MS). Three scion peach cultivars, 'UFO-3', 'Flariba' and 'Alice Bigi', and the peach × almond rootstocks 'Garnem' and 'GF677' were cultured in two different media, Murashige and Skoog supplemented with plant growth regulators (PGRs) (regeneration medium) and without PGRs (control medium), in order to study the effects of the media and/or genotypes in the endogenous hormones content and their role in organogenesis. The highest regeneration rate was obtained with the peach × almond rootstocks and showed a lower content of Z, IAA, ABA, ACC and JA. Only Z, ZR and IAA were affected by the action of the culture media. This study shows which hormones are external PGRs-dependent and what is the weight of the genotype and hormones in peach organogenesis that provide an avenue to manipulate in vitro organogenesis in peach. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

Slowing ash mortality: a potential strategy to slam emerald ash borer in outlier sites

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Deborah G. McCullough; Nathan W. Siegert; John Bedford

2009-01-01

Several isolated outlier populations of emerald ash borer (Agrilus planipennis Fairmaire) were discovered in 2008 and additional outliers will likely be found as detection surveys and public outreach activities...

Population biology of emerald ash borer and its natural enemies in China

Science.gov (United States)

Houping Liu; Leah S. Bauer; Tonghai Zhao; Ruitong Gao

2008-01-01

Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), also known as emerald ash borer (EAB), was first discovered in Michigan and Ontario, Canada, in 2002 following investigations of declining and dying ash trees (Fraxinus spp.). Agrilus planipennis has also spread to Ohio, Indiana, Maryland, Virginia,...

Modeling potential movements of the emerald ash borer: the model framework

Science.gov (United States)

Louis R. Iverson; Anantha Prasad; Jonathan Bossenbroek; Davis Sydnor; Mark W. Schwartz

2010-01-01

The emerald ash borer (EAB, Agrilus planipennis Fairmaire) is threatening to decimate native ashes (Fraxinus spp.) across North America and, so far, has devastated ash populations across sections of Michigan, Ohio, Indiana, and Ontario. We are attempting to develop a computer model that will predict EAB future movement by adapting...

Emerald ash borer in North America: a research and regulatory challenge

Science.gov (United States)

David Cappaert; Deborah G. McCullough; Therese M. Poland; Nathan W. Siegert

2005-01-01

The saga of the emerald ash borer, Agrilus planipennis Fairmare (Coleoptera: Buprestidae), in North America began on 25 June 2002, when five entomologists representing Michigan State University (MSU), the Michigan Department of Natural Resources (MDNR), and the U.S. Department of Agriculture, Animal and Plant Health Inspection Service (USDA APHIS)...

Pre-symptomatic transcriptome changes during cold storage of chilling sensitive and resistant peach cultivars to elucidate chilling injury mechanisms

OpenAIRE

Puig, Clara Pons; Dagar, Anurag; Marti Ibanez, Cristina; Singh, Vikram; Crisosto, Carlos H; Friedman, Haya; Lurie, Susan; Granell, Antonio

2015-01-01

Background: Cold storage induces chilling injury (CI) disorders in peach fruit (woolliness/mealiness, flesh browning and reddening/bleeding) manifested when ripened at shelf life. To gain insight into the mechanisms underlying CI, we analyzed the transcriptome of 'Oded' (high tolerant) and 'Hermoza' (relatively tolerant to woolliness, but sensitive to browning and bleeding) peach cultivars at pre-symptomatic stages. The expression profiles were compared and validated with two previously analy...

Molecular cloning of plane pollen allergen Pla a 3 and its utility as diagnostic marker for peach associated plane pollen allergy.

Science.gov (United States)

Wangorsch, A; Larsson, H; Messmer, M; García-Moral, A; Lauer, I; Wolfheimer, S; Schülke, S; Bartra, J; Vieths, S; Lidholm, J; Scheurer, S

2016-05-01

Non-specific lipid transfer proteins (nsLTP) are considered to provoke allergic symptoms to plane tree pollen, which are frequently associated with peach allergy. The objective was to clone the cDNA of plane pollen nsLTP Pla a 3, to characterize IgE-binding and allergenic potency of recombinant Pla a 3 in comparison to its natural counterpart and peach nsLTP Pru p 3. Natural Pla a 3 was purified from plane pollen and analysed by mass spectrometry (MS). Recombinant Pla a 3 was characterized by SDS-PAGE and CD spectroscopy. Specific IgE to extract, components of plane pollen and Pru p 3 was measured by ImmunoCAP in sera of patients allergic to either plane pollen (n = 10), peach (n = 15) or both (n = 15). Biological potency of the proteins was investigated by in vitro mediator release assays and IgE cross-reactivity by competitive ELISA. Two Pla a 3 isoforms were identified. Recombinant Pla a 3 showed high purity, structural integrity, IgE-binding capacity comparable to nPla a 3 and biological potency. Sensitization to plane pollen extract was confirmed in 24/25 plane pollen allergics. The frequency of sensitization to Pla a 3 was 53% among patients allergic to both plane pollen and peach and 10% among plane pollen allergics tolerating peach where most patients were sensitized to Pla a 1. Pla a 3 and Pru p 3 showed strong bi-directional IgE cross-reactivity in patients allergic to peach and plane pollen, but not in peach allergics tolerating plane pollen. Levels of IgE-binding were generally higher to Pru p 3 than to Pla a 3. Sensitization to Pla a 3 is relevant in a subgroup of plane pollen allergics with concomitant peach allergy. IgE testing with Pla a 3 may serve as a marker to identify plane pollen allergic patients at risk of LTP-mediated food reactions and thereby improve in vitro diagnostic procedures. © 2016 John Wiley & Sons Ltd.

Cryopreservation of peach palm zygotic embryos.

Science.gov (United States)

Steinmacher, Douglas A; Saldanha, Cleber W; Clement, Charles R; Guerra, Miguel P

2007-01-01

Cryopreservation is a safe and cost-effective option for long-term germplasm conservation of non-orthodox seed species, such as peach palm (Bactris gasipaes). The objective of the present study was to establish a cryopreservation protocol for peach palm zygotic embryos based on the encapsulation-dehydration technique. After excision, zygotic embryos were encapsulated with 3 percent sodium alginate plus 2 M glycerol and 0.4 M sucrose, and pre-treated or not with 1 M sucrose during 24 h, followed by air-drying. Fresh weight water contents of beads decreased from 83 percent and 87 percent to 18 percent and 20 percent for pre-treated or non-pretreated beads, respectively, after 4 h of dehydration. Sucrose pre-treatment at 1 M caused lower zygotic embryo germination and plantlet height in contrast to non-treated beads. All the variables were statistically influenced by dehydration time. Optimal conditions for recovery of cryopreserved zygotic embryos include encapsulation and dehydration for 4 h in a forced air cabinet to 20 percent water content, followed by rapid freezing in liquid nitrogen (-196 degree C) and rapid thawing at 45 degree C. In these conditions 29 percent of the zygotic embryos germinated in vitro. However, plantlets obtained from dehydrated zygotic embryos had stunted haustoria and lower heights. Histological analysis showed that haustorium cells were large, vacuolated, with few protein bodies. In contrast, small cells with high nucleus:cytoplasm ratio formed the shoot apical meristem of the embryos, which were the cell types with favorable characteristics for survival after exposure to liquid nitrogen. Plantlets were successfully acclimatized and showed 41+/-9 percent and 88+/-4 percent survival levels after 12 weeks of acclimatization from cryopreserved and non-cryopreserved treatments, respectively.

Dispersal of the emerald ash borer, Agrilus planipennis, in newly-colonized sites

Science.gov (United States)

Rodrigo J. Mercader; Andrew M. Siegert; Andrew M. Liebhold; Deborah G. McCullough

2009-01-01

Emerald ash borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) is an invasive forest insect pest threatening more than 8 billion ash (Fraxinus spp.) trees in North America. Development of effective survey methods and strategies to slow the spread of A. planipennis requires an understanding of dispersal...

Mortality and reduced brood production in walnut twig beetles, Pityophthorus juglandis (Coleoptera: Curculionidae), following exposure to commercial strains of entomopathogenic fungi Beauveria bassiana and Metarhizium brunneum

Science.gov (United States)

Louela A. Castrillo; Albert E. Mayfield; Michael H. Griggs; Robert Camp; Bryan Mudder; Adam Taylor; John D. Vandenberg

2017-01-01

Thousand cankers disease (TCD), caused by the walnut twig beetle (WTB), Pityophthorus juglandis, and its associated fungal symbiont, Geosmithia morbida, is a disease of economic and ecological concern on eastern black...

Imidacloprid concentration effects on adult emerald ash borer: a greenhouse study

Science.gov (United States)

David Cappaert; Deborah G. McCullough; Therese M. Poland; Phil Lewis; John Molongoski

2008-01-01

Imidacloprid is the active ingredient of many widely used products applied to control the emerald ash borer (EAB), Agrilus planipennis Fairmaire, in valuable urban trees. Systemic treatment with imidacloprid is typically made in the spring to reduce the number of larvae that would otherwise be generated by oviposition during the summer. Substantial...

Carbohydrate-free peach (Prunus persica and plum (Prunus domestica juice affects fecal microbial ecology in an obese animal model.

Directory of Open Access Journals (Sweden)

Giuliana D Noratto

Full Text Available BACKGROUND: Growing evidence shows the potential of nutritional interventions to treat obesity but most investigations have utilized non-digestible carbohydrates only. Peach and plum contain high amounts of polyphenols, compounds with demonstrated anti-obesity effects. The underlying process of successfully treating obesity using polyphenols may involve an alteration of the intestinal microbiota. However, this phenomenon is not well understood. METHODOLOGY/PRINCIPAL FINDINGS: Obese Zucker rats were assigned to three groups (peach, plum, and control, n = 10 each, wild-type group was named lean (n = 10. Carbohydrates in the fruit juices were eliminated using enzymatic hydrolysis. Fecal samples were obtained after 11 weeks of fruit or control juice administration. Real-time PCR and 454-pyrosequencing were used to evaluate changes in fecal microbiota. Over 1,500 different Operational Taxonomic Units at 97% similarity were detected in all rats. Several bacterial groups (e.g. Lactobacillus and members of Ruminococcacea were found to be more abundant in the peach but especially in the plum group (plum juice contained 3 times more total polyphenolics compared to peach juice. Principal coordinate analysis based on Unifrac-based unweighted distance matrices revealed a distinct separation between the microbiota of control and treatment groups. These changes in fecal microbiota occurred simultaneously with differences in fecal short-chain acids concentrations between the control and treatment groups as well as a significant decrease in body weight in the plum group. CONCLUSIONS: This study suggests that consumption of carbohydrate-free peach and plum juice has the potential to modify fecal microbial ecology in an obese animal model. The separate contribution of polyphenols and non-polyphenols compounds (vitamins and minerals to the observed changes is unknown.

Coffee Berry Borer Joins Bark Beetles in Coffee Klatch

Science.gov (United States)

Jaramillo, Juliana; Torto, Baldwyn; Mwenda, Dickson; Troeger, Armin; Borgemeister, Christian; Poehling, Hans-Michael; Francke, Wittko

2013-01-01

Unanswered key questions in bark beetle-plant interactions concern host finding in species attacking angiosperms in tropical zones and whether management strategies based on chemical signaling used for their conifer-attacking temperate relatives may also be applied in the tropics. We hypothesized that there should be a common link in chemical signaling mediating host location by these Scolytids. Using laboratory behavioral assays and chemical analysis we demonstrate that the yellow-orange exocarp stage of coffee berries, which attracts the coffee berry borer, releases relatively high amounts of volatiles including conophthorin, chalcogran, frontalin and sulcatone that are typically associated with Scolytinae chemical ecology. The green stage of the berry produces a much less complex bouquet containing small amounts of conophthorin but no other compounds known as bark beetle semiochemicals. In behavioral assays, the coffee berry borer was attracted to the spiroacetals conophthorin and chalcogran, but avoided the monoterpenes verbenone and α-pinene, demonstrating that, as in their conifer-attacking relatives in temperate zones, the use of host and non-host volatiles is also critical in host finding by tropical species. We speculate that microorganisms formed a common basis for the establishment of crucial chemical signals comprising inter- and intraspecific communication systems in both temperate- and tropical-occurring bark beetles attacking gymnosperms and angiosperms. PMID:24073204

Estimation of glycaemic index of peach palm (Bactris gasipaes) cooked fruits and chips, and pitahaya (Hylocereus spp.) pulp.

Science.gov (United States)

Jiménez, Gin; Gómez, Georgina; Pérez, Ana M; Blanco-Metzler, Adriana

2012-09-01

The glycaemic index (GI) is a physiological measure of a food's potential to increase postprandial blood glucose, as compared to the effect produced by food taken as reference, such as glucose or white bread. Currently researchers and consumers are interested in low GI foods, since their consumption is associated with better weight control and reduced risk of incidence of chronic diseases, like diabetes. In the present study, the GI value for peach palm cooked fruit, peach palm chips and pitahaya pulp was estimated. The methodology established by the FAO/WHO for determining the GI of food was used. A total of 12 healthy, non-smoking volunteers were selected and they ingested the fore mentioned foods on different occasions, in 25 g portions of available carbohydrates, after 12-14 h overnight fast. Blood glucose levels were measured in 30 min intervals up to 120 min after ingestion. Average GI value was 48 +/- 11 for the pitahaya pulp and 35 +/- 6 for the peach palm cooked fruit, which may be classified as low glyceamic index foods. The GI of peach palm chips was 60 +/- 7, corresponding to a food with a moderate GI. The processing for producing the chips caused an increase in the GI value when compared to the cooked fruit, probably because the stages of miIling, moulding and baking promote availability of starch during hydrolysis by the digestive enzymes.

Station blackout calculations for Peach Bottom

International Nuclear Information System (INIS)

Hodge, S.A.

1985-01-01

A calculational procedure for the Station Blackout Severe Accident Sequence at Browns Ferry Unit One has been repeated with plant-specific application to one of the Peach Bottom Units. The only changes required in code input are with regard to the primary continment concrete, the existence of sprays in the secondary containment, and the size of the refueling bay. Combustible gas mole fractions in the secondary containment of each plant during the accident sequence are determined. It is demonstrated why the current state-of-the-art corium/concrete interaction code is inadequate for application to the study of Severe Accident Sequences in plants with the BWR MK I or MK II containment design

Influence of age and diet on the performance of Cephalonomia stephanoderis (Hymenoptera, Bethylidae a parasitoid of the coffee berry borer, Hypothenemus hampei (Coleoptera, Curculionidae

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Jaime Gómez

2012-03-01

Full Text Available The importance of age and feeding on the performance of Cephalonomia stephanoderis (Hymenoptera, Bethylidae, a parasitoid of the coffee berry borer, Hypothenemus hampei (Coleoptera, Curculionidae was investigated in the laboratory. Groups of female parasitoids were subject to the following treatments: a group fed during one, five and ten days after emergence of adults with coffee borer larvae; another group fed only with honey solution during five days after emergence; and as a control, a third group was kept without food for five days. At the end of each treatment, survivorship, parasitoid activity (walking and flying capacity in an arena, search capacity for finding coffee borer-infested berries, host feeding and oviposition (on immature hosts, were assessed. Unfed females showed a significant decrease in survivorship compared to individuals that were fed. The type of meal (insects or honey did not significantly influence parasitoid activity, search and oviposition capacities. Females fed with honey solution significantly consumed less immature coffee borers. Younger females (one day old walked and flew out of the arena significantly faster than older ones (5 and 10 days old. Implications of these results are discussed on the performance of C. stephanoderis as a biological control agent of the coffee berry borer.

Differential resistance reaction of maize genotypes to maize stem borer (Chilo partellus Swinhoe at Chitwan, Nepal

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Ghanashyam Bhandari

2016-12-01

Full Text Available Maize stem borer (MSB, Chilo partellus Swinhoe, Lepidoptera: Pyralidae is one of the most important insect pest of maize in Nepal. Host plant resistance is the cost-effective, ecologically sound and stable approach to reduce damage by stem borers. Forty four maize genotypes were screened for resistance to maize stem borer at the research field of National Maize Research Program, Rampur during spring seasons (March to June of two consecutive years 2013 and 2014. The maize genotypes were evaluated in randomized complete block design with three replications and data were collected on foliar damage rating, tunnel length and number of exit holes made by the borer. The foliar damage and tunnel length damage were significant for genotypes for both the years. The exit holes were not significant in 2013 but significant in 2014 ranging from 2-6 scale. The foliar rating ranged from 2 to 5.5 in 2013 and 1.1 to 4.5 in 2014 on a 1-9 rating scale. The highly resistant genotypes (10 cm scale. The least susceptible genotypes (<5 cm were RampurSO3F8, RampurSO3FQ02 and RampurS10F18. The genotypes having least exit holes (2.0 in 2014 were RampurSO3F8, RampurSO3FQ02, RampurS10F18. Thus less damage parameters were observed in R-POP-2, RML-5/RML-8, RampurSO3F8, RampurSO3FQ02 and RampurS10F18 and therefore they can be used as parents or as sources of resistance in breeding program.

Repeatability, correlation and path analysis of physical and chemical characteristics of peach fruits

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Rosana Gonçalves Pires Matias

2014-12-01

Full Text Available This study aimed to determine the number of measurements necessary to evaluate physical and chemical characteristics of peach fruits, study the relationships between them and their direct and indirect effects on the content of ascorbic acid and total carotenoids. The characteristics skin and pulp color, fruit weight, suture, equatorial and polar diameters, firmness, soluble solids (SS, titratable acidity (TA, SS/TA ratio, ascorbic acid and total carotenoids were evaluated in 39 cultivars of peach and 3 cultivars of nectarine from the orchard of the Universidade Federal de Viçosa. The repeatability coefficient was estimated by ANOVA and CPCOR. Phenotypic correlation coefficients (rf were estimated and, after the multicollinearity diagnostics, they were unfolded to direct and indirect effects of the explanatory variables on the response variable using path analysis. There was agreement on the magnitude of repeatability coefficients obtained by the two methods; however, they varied among the 14 characteristics. The highest correlations were found between FW, SD, ED and PD. Seven fruits are sufficient to evaluate the physical and chemical characteristics of peach with a correlation coefficient of 90%. The characteristics considered in the path diagrams (b* skin, hº skin, b* pulp, hº pulp, ED, PD, FIR, SS, SS/AT and TC are not the main determinants of the ascorbic acid. The yellow hue of the pulp (hº pulp has the potential to be used in indirect selection for total carotenoids.

Screening of tomato genotypes for resistance to tomato fruit borer (helicoverpa armiger hubner) in Pakistan

International Nuclear Information System (INIS)

Sajjad, M.; Ashfaq, M.; Suhail, A.

2011-01-01

Tomato genotypes viz., Roma Local, Rio Grande, Tanja, Chico III, Long Tipped, Red-Top, FS-8001, FS-8002, Tropic, Pakit, Peelo, NARC-1, Roma VFN, Pant Bahr, Ebein, Nova Mech, Rockingham, Nagina, Shalkot-96, Pomodoro, Manik, Gressilesse, Nadir, Early Mech, Tommy, Pusha Rubi, Tropic boy, Big Long, Sahil, Sun 6002, Money-Maker and Royesta were evaluated to screen out the suitable resistant/susceptible genotypes against the fruit borer in Pakistan. The results imparted that the percentage of fruit infestation and larval population per plant on tested genotypes of tomato varied significantly. Roma VF, NARC-1 and FS-8002 were categorized as susceptible genotypes with fruit infestation (37.69%, 37.08% and 36.41%, respectively) and larval population per plant (1.02%, 1.02% and 0.84 %, respectively). Whereas, the genotypes Sahil, Pakit and Nova Mecb had fruit infestation (12.30%, 13.14% and 13.96%, respectively) and larval population per plant (0.42%, 0.42% and 0.43%, respectively) and declared as resistant genotypes to tomato fruit borer. Lower values of host plant susceptibility indices (HPSI) were recorded on resistant genotypes. Sahil, Pakit and Nova Mecb could be used as a source of resistance for developing tomato genotypes resistant to tomato fruit borer. (author)

Formulation of a peach ice cream as potential symbiotic food

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Fernando Josué VILLALVA

Full Text Available Abstract Today’s population increasingly demands and consumes healthy products. For this reason, the food industry has been developing and marketing food with added bioactive components. The aim of this work was to formulate a peach ice cream reduced in calories with an added probiotic (Bifidobacterium lactis Bb-12 and prebiotics (inulin, and to evaluate its sensory quality and acceptability as potential symbiotic food. The moisture content was 76.47%; 7.14% protein; 0.15% fat; 6.37%; carbohydrates; 9.87% inulin; 1.22% ash; 0.201% calcium, 0.155% phosphorus and 0.168% sodium. On the first and 21th day of storage counts of B. lactis Bb – 12 was 4 x 108 CFU/mL and 1.5 x 107 CFU/mL, respectively. It was possible to formulate a peach ice cream reduced in calories, fat, and sugar and with potential symbiotic effect, by addition of B. lactis Bb – 12. A product with suitable organoleptic characteristics, creamy texture, peachy colour, taste and flavour, and no ice crystals was obtained. This ice cream would be a suitable food matrix to incorporate prebiotic and probiotic ingredients as a potential symbiotic food.

Screening of different insecticides against maize shoot fly atherigona soccata (Rond.) and maize borer. chilo partellus (swinh.)

International Nuclear Information System (INIS)

Shahid, M.A.; Rana, Z.A.; Haq, I.; Tariq, H.

2010-01-01

Field studies were carried out in the research area of the Ayub Agricultural Research Institute, Faisalabad to determine the most effective maize seed treatment against maize shoot fly Atherigona soccata (Rond.) and insecticide against maize borer Chilo partellus (Swinh.) Trials were conducted following RCBD and replicated three times during 2005-2006. Two seed treatments Confider (imidacloprid) 70 WS and pensidor 72% WP (5 and 7 mg/kg seed) along with Confider (imidaclorid) 200 SC at the rate 40 ml/acre in the trial against maize shoot fly whereas, flubendiamide 48%, emamection 1.9 EC, spinosad 240 EC. carbofuran 3 G, indoxacarb 150 SC, alphacypermethrine 20 EC, monomehypo 5 G, bifenthrin 10 EC, cartap 4G, cyhalothrine 2.5 EC, cypermethrin 10 EC at the rate 20 ml, 150 ml, 40 ml, 8 kg, 150 ml, 200 ml, 5 kg, 150 ml, 6 kg. 250 ml and 300 ml per acre against maize borer were treated keeping one plo ast untreated check. Treatments were repeated as borer infestation reached above 5% level. All the seed treatments showed significant control of maize shoot fly in spite of dose 5 or 7 mg/kg seed along with foliar spray of confider 200 SC. The insecticides viz. flubendiamide 48% SC. emamectin 1.9 EC, spinosad 240 EC and carbofuran 3 G. indoxacarb 150 SC. alpha cypermethrin 20 EC, not only responded highest yield 5765, 5294, 5289, 5215, 5168 and 5025 kg/ha respectively but also manage the maize borer below ETL. (author)

Studies on control of yellow stem borer, Tryporyza incertulas, a serious pest of paddy. Part of a coordinated programme on the use of pest management with emphasis on rice insects

International Nuclear Information System (INIS)

Rahalkar, G.W.

1982-03-01

Egg parasitism is the chief biotic factor regulating paddy stem borer populations. However, effective suppression of the borer through their natural enemies is not evident in practice. Studies were undertaken to evaluate the feasibility of releasing sterile yellow stem borer females into the field to increase host egg densities as these females would lay nonviable eggs. (a) Paddy fields were surveyed for the incidence of egg parasitism. (b) Attempts were made to rear yellow stem borer larvae on cut stem pieces of field grown rice plants. (c) Trichogramma japonicum, the natural egg parasite of the stem borer was reared in the laboratory using eggs of Corcyra cephalonica and Ephestia cautella as alternate host material. Mass rearing of yellow stem borer larvae on paddy plants grown under laboratory conditions does not appear practically feasible. Borer larvae reared on cut stem pieces with intact roots did not yield satisfactory adults as far as their reproductive behaviour was concerned. Though some of the dietary compositions contained natural host material, they failed to support the larval establishment. Probably some highly specific phagostimulants need to be incorporated in the diets. T. japonicum could be reared on radiation killed Ephestia eggs. The female parasite was unable to discriminate between eggs laid by radiation sterilized host females and those laid by normal ones

Phytosanitary irradiation of peach fruit moth (Lepidoptera: Carposinidae) in apple fruits

International Nuclear Information System (INIS)

Zhan, Guoping; Li, Baishu; Gao, Meixu; Liu, Bo; Wang, Yuejin; Liu, Tao; Ren, Lili

2014-01-01

Peach fruit moth, Carposina sasakii Matsumura, is a serious pest of many pome and stone fruits and presents a quarantine problem in some export markets. It is widely distributed in pome fruit production areas in China, Japan, Korea, North Korea and the Far Eastern Federal District of Russia. In this investigation, gamma radiation dose–response tests were conducted with late eggs (5-d-old) and various larval stages, followed by large-scale confirmatory tests on the most tolerant stage in fruit, the fifth instar. The dose-response tests, with the target radiation dose of 20 (late eggs), 40, 60, 80, 100, 120, 140, and 160 Gy (late fifth instars in vitro) respectively applied to all stages, showed that the tolerance to radiation increased with increasing age and developmental stage. The fifth instar (most advanced instar in fruits) was determined to be the most tolerant stage requiring an estimated minimum absorbed dose of 208.6 Gy (95% CI: 195.0, 226.5 Gy) to prevent adult emergence at 99.9968% efficacy (95% confidence level). In the confirmatory tests, irradiation was applied to 30,850 late fifth instars in apple fruits with a target dose of 200 Gy (171.6–227.8 Gy measured), but only 4 deformed adults emerged that died 2 d afterwards without laying eggs. A dose of 228 Gy may be recommended as a phytosanitary irradiation treatment under ambient atmosphere for the control of peach fruit moth on all commodities with an efficacy of 99.9902% at 95% confidence level. - Highlights: • Dose–response tests were conducted on eggs and all larval stages. • Fifth instar is the most tolerant stage that could be shipped in fruits. • None normal-looking adult emerged from 30,850 fifth instars in confirmatory tests. • A minimum of 228 Gy is suggested for phytosanitary irradiation of peach fruit moth

Traps craft with attractive alcoholics in the control of the coffee berry borer, Hypothenemus hampei (Ferrari 1867 in Cologne Bolinda, Caranavi

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Quispe-Condori Rosalía

2015-05-01

Full Text Available The coffee is one of the main cultivated crops in the Caranavi region, among the biotic stress factors; the borer of the coffee (Hypothenemus hampei is the one that affects significantly and negatively. In order to finding alternative practical and economic for the control of the pest, it was carried this investigation in the “Bolinda” Colony of the Caranavi Municipality La Paz-Bolivia, the trial was established under a completely random, design with two study factors, e three replications, 1.5 ha distributed at random in the coffee plantations. Three types of traps were built handmade. These were, INIA, ECOIAPAR and TRAP BORER, in combination with the attractive mixtures of alcohols methanol (M and ethanol (E in the proportion of 3: 1; it mixes 3: 1: 1 M-E+milled coffee, 2: 1 M-E and commercial alcohol as check. Borer/trap/attractive capture was evaluated. He she was highly significant statistical differences among them. The biggest captures female adults of Hipothenemus hampei were presented in the proportion 2: 1 of M-E and ECOIAPAR trap (T8 was identified as the most efficient and economic, being able to capture 4877 borer, with a cost trap (1.50 Bs and the attractive (2.20 Bs, continued by the T2 with the same cost (proportion 3: 1 of M-E and INIA trap with 159 borer and the treatments witnesT9, T5 and T1 (commercial alcohol they obtained smaller captures with 23, 35 and 38 drills, which means that it is not effective for the control. The costs of the implementation of traps the marginal cost of 40 Bolivianos/ha. The results obtained in the study show the biggest borer captures were in December and January, the use of handmade traps constitutes an alternative for the control in the period of postharvest, a more practical and economic method, feasible for the producers.

Model with Peach Bottom Turbine trip and thermal-Hydraulic code TRACE V5P3

International Nuclear Information System (INIS)

Mesado, C.; Miro, R.; Barrachina, T.; Verdu, G.

2014-01-01

This work is the continuation of the work presented previously in the thirty-ninth meeting annual of the Spanish Nuclear society. The semi-automatic translation of the Thermo-hydraulic model TRAC-BF1 Peach Bottom Turbine Trip to TRACE was presented in such work. This article is intended to validate the model obtained in TRACE, why compare the model results result from the translation with the Benchmark results: NEA/OECD BWR Peach Bottom Turbine Trip (PBTT), in particular is of the extreme scenario 2 of exercise 3, in which there is SCRAM in the reactor. Among other data present in the (transitional) Benchmark , are: total power, axial profile of power, pressure Dome, total reactivity and its components. (Author)

Microbiological analysis of peach palm in natura submitted to 60Co radiation

International Nuclear Information System (INIS)

Silva, Priscila V.; Araujo, Michel M.; Nunes, Thaise C.F.; Costa, Helbert S.F.; Villavicencio, Anna Lucia C.H.; Hojeije, Khalil Y.

2009-01-01

The palm tree (Bactris gasipaes Kunth) is a species with high potential benefits, because of the nutritional value of its fruits that could be used both in human and animals feeding and mainly for peach palm extraction. It represents a great source of dietary fiber and a moderate source of magnesium and iron. Food irradiation is a worldwide technology that aims to improve the product quality, in order to eliminate diverse microorganisms that can spoil the food. Radiation processing, in the recommended doses, causes very few chemical alterations and nutritional losses in foods, being considered insignificant and/or similar to other food treatments. The objective of this study was to evaluate the effect of irradiation on microbiological counts of mesophilic aerobic in the peach palm in natura. Samples were irradiated with 1.0 and 1.5 kGy using a 60 Co multipurpose irradiator. Radiation treatment appeared to be a useful alternative to reduce microbial contamination in the samples analyzed. (author)

Effects of Different Planting Times of Different Rice Cultivars on Control of the Striped Stem Borer (Chilo suppressalis (Lepidoptera: Pyralidae

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T. Oskou

2016-06-01

Full Text Available Introduction: Rice (Oryza sativa L. is one of the world’s most important staple food crops. In Asia, it is the main item of the diet of 3.5 billion people. Rice stem borers are common insect pests in many rice growing countries. Striped stem borer (Chilo suppressalis Walker, belonging to Lepidoptera and family of Pyralidae is the most important rice pest in the Northern Iran. Stem borer larva damages rice stem and disturbs nutrient translocation from root to leaf. As the result, tillers in vegetative stage died, which is called dead heart. When larva infests generative stage, it causes empty panicle, which is called white head. Integrated pest management (IPM practices for controlling the stem borers in Iran have not been fully implemented because of limited control technologies which are available. Farmers often rely on heavily insecticide application, although many insecticide applications are not effective. Therefore, many physical and cultural practices have been suggested, including adjustment of planting time to escape the plant from heavy pest. Materials and Methods: The study was conducted in deputy of rice research institute in Amol, Mazandaran, Iran, during 2011-2012. The experiment was arranged in split plot design with planting time as main plot and cultivar as subplot and was replicated three times. Three planting times tested were the first planting time i.e. 15 days before farmers’ planting time, the second planting time (simultaneously with farmers’ planting time, and the third planting time (15days after farmers’ planting time. Six rice cultivars tested, representing three types of rice cultivar, Tarom and Kohsar (early maturity cultivars, Shirodi and Fajr (medium maturity cultivars, Neda and Nemat (late maturity cultivar. Rice seedlings were transplanted at 25 cm planting distance in a 3 m x 9 m plot size. Weeding and fertilization were done as recommended. No insecticide was applied. Dead hearts and white heads were

Genetic transformation of Fraxinus spp. for resistance to the emerald ash borer

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Paula M. Pijut; Rochelle R. Beasley; Kaitlin J. Palla

2010-01-01

The emerald ash borer (EAB; Agrilus planipennis Fairmaire) (Coleoptera; Buprestidae) is a wood-boring beetle that poses substantial risk to the ash resource in North America. Ash species native to the United States and known to be susceptible to EAB are Fraxinus pennsylvanica (green ash), F. americana (white ash...

Influence of host age on critical fitness parameters of Spathius galinae (Hymenoptera: Braconidae), a new parasitoid of the emerald ash borer (Coleoptera: Buprestidae).

Science.gov (United States)

Watt, Timothy J; Duan, Jian J

2014-08-01

Spathius galinae Belokobylskij and Strazenac (Hymenoptera: Braconidae) is a recently discovered gregarious idiobiont larval ectoparasitoid currently being evaluated for biological control against the invasive emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) in the United States. To aid in the development of laboratory rearing protocols, we assessed the influence of various emerald ash borer stages on critical fitness parameters of S. galinae. We exposed gravid S. galinae females to emerald ash borer host larvae of various ages (3.5, 5, 7, and 10 wk post egg oviposition) that were reared naturally in tropical (evergreen) ash (Fraxinus uhdei (Wenzig) Lingelsh) logs, or to field-collected, late-stage emerald ash borers (nonfeeding J-shaped larvae termed "J-larvae," prepupae, and pupae) that were artificially inserted into green ash logs. When exposed to larvae in tropical ash logs, S. galinae attacked 5 and 7 wk hosts more frequently (68-76%) than 3.5 wk (23%) and 10 wk (12%) hosts. Subsample dissections of the these logs revealed that 3.5, 5, 7 and 10 wk host logs contained mostly second, third, fourth, and J-larvae, respectively, that had already bored into the sapwood for diapause. No J-larvae were attacked by S. galinae when naturally reared in tropical ash logs. When parasitized by S. galinae, 7 and 10 wk hosts produced the largest broods (approximately 6.7 offspring per parasitized host), and the progenies that emerged from these logs had larger anatomical measurements and more female-biased sex ratios. When exposed to emerald ash borer J-larvae, prepupae, or pupae artificially inserted into green ash logs, S. galinae attacked 53% ofJ-larvae, but did not attack any prepupae or pupae. We conclude that large (fourth instar) emerald ash borer larvae should be used to rear S. galinae.

Detection of emerald ash borer, Agrilus planipennis, at low population density

Science.gov (United States)

Melissa J. Porter; Michael D. Hyslop; Andrew J. Storer

2011-01-01

The exotic emerald ash borer (EAB), Agrilus planipennis (Coleoptera: Buprestidae), was first discovered in North America in Detroit, MI, in 2002. This beetle has killed millions of ash trees in several states in the United States and in Canada, and populations of this insect continue to be detected. EAB is difficult to detect when it invades new...

Sensory profile of eleven peach cultivars Perfil sensorial de onze cultivares de pêssegos

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Francine Lorena Cuquel

2012-03-01

Full Text Available The goal of this study was to evaluate the sensory profile of eleven peach cultivars grown in an experimental orchard located in the city of Lapa (PR, Brazil in two seasons. The peach cultivars analyzed were Aurora I, Chimarrita, Chiripá, Coral, Eldorado, Granada, Leonense, Maciel, Marli, Premier, and Vanguarda. The sensory analysis was performed by previously trained panelists; 20 of them in the first season and 10 in the second season. The sensory evaluation was performed using Quantitative Descriptive Analysis, in which the following attributes were measured: appearance, aroma, flesh color, flesh firmness, flavor, and juiciness. The results showed preference for sweet, soft, and juicy fruits. Chimarrita, Chiripá, and Coral fruits showed better sensorial performance than the other peach cultivars. It was also verified that the analysis of the attributes aroma, flesh firmness, and flavor is enough for performing the sensory profile of peach fruits for in natura consumption.Este trabalho teve como objetivo avaliar o perfil sensorial de onze cultivares de pêssego produzidos em duas safras em um pomar experimental implantado na Lapa (PR, Brasil. Os cultivares analisados foram Aurora I, Chimarrita, Chiripá, Coral, Eldorado, Granada, Leonense, Maciel, Marli, Premier e Vanguarda. As análises sensoriais foram realizadas por julgadores previamente treinados, sendo 20 julgadores na primeira safra e 10 na segunda. O método de avaliação empregado foi a Análise Descritiva Quantitativa na qual foram mensurados os atributos aparência, aroma, cor de polpa, firmeza de polpa, sabor e suculência dos frutos. Os resultados obtidos demonstraram a preferência por frutos de sabor adocicado, com polpa macia e suculenta. Os cultivares Chimarrita, Chiripá e Coral obtiveram o melhor desempenho nas análises sensoriais. Foi verificado ainda que os atributos aroma, firmeza de polpa e sabor são considerados suficientes para a avaliação do perfil sensorial de

Peach Bottom transient analysis with BWR TRACB02

International Nuclear Information System (INIS)

Alamgir, M.; Sutherland, W.A.

1984-01-01

TRAC calculations have been performed for a Turbine Trip transient (TT1) in the Peach Bottom BWR power plant. This study is a part of the qualification of the BWR-TRAC code. The simulation is aimed at reproducing the observed thermal hydraulic behavior in a pressurization transient. Measured core power is an input to the calculation. Comparison with data show the code reasonably well predicts the generation and propagation of the pressure waves in the main steam line and associated pressurization of the reactor vessel following the closure of the turbine stop valve

Peach Bottom HTGR decommissioning and component removal

International Nuclear Information System (INIS)

Kohler, E.J.; Steward, K.P.; Iacono, J.V.

1977-07-01

The prime objective of the Peach Bottom End-of-Life Program was to validate specific HTGR design codes and predictions by comparison of actual and predicted physics, thermal, fission product, and materials behavior in Peach Bottom. Three consecutive phases of the program provide input to the HTGR design methods verifications: (1) Nondestructive fuel and circuit gamma scanning; (2) removal of steam generator and primary circuit components; and (3) Laboratory examinations of removed components. Component removal site work commenced with establishment of restricted access areas and installation of controlled atmosphere tents to retain relative humidity at <30%. A mock-up room was established to test and develop the tooling and to train operators under simulated working conditions. Primary circuit ducting samples were removed by trepanning, and steam generator access was achieved by a combination of arc gouging and grinding. Tubing samples were removed using internal cutters and external grinding. Throughout the component removal phase, strict health physics, safety, and quality assurance programs were implemented. A total of 148 samples of primary circuit ducting and steam generator tubing were removed with no significant health physics or safety incidents. Additionally, component removal served to provide access fordetermination of cesium plateout distribution by gamma scanning inside the ducts and for macroexamination of the steam generator from both the water and helium sides. Evaluations are continuing and indicate excellent performance of the steam generator and other materials, together with close correlation of observed and predicted fission product plateout distributions. It is concluded that such a program of end-of-life research, when appropriately coordinated with decommissioning activities, can significantly advance nuclear plant and fuel technology development

Colour change of twig-mimicking peppered moth larvae is a continuous reaction norm that increases camouflage against avian predators

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Amy Eacock

2017-11-01

Full Text Available Camouflage, and in particular background-matching, is one of the most common anti-predator strategies observed in nature. Animals can improve their match to the colour/pattern of their surroundings through background selection, and/or by plastic colour change. Colour change can occur rapidly (a few seconds, or it may be slow, taking hours to days. Many studies have explored the cues and mechanisms behind rapid colour change, but there is a considerable lack of information about slow colour change in the context of predation: the cues that initiate it, and the range of phenotypes that are produced. Here we show that peppered moth (Biston betularia larvae respond to colour and luminance of the twigs they rest on, and exhibit a continuous reaction norm of phenotypes. When presented with a heterogeneous environment of mixed twig colours, individual larvae specialise crypsis towards one colour rather than developing an intermediate colour. Flexible colour change in this species has likely evolved in association with wind dispersal and polyphagy, which result in caterpillars settling and feeding in a diverse range of visual environments. This is the first example of visually induced slow colour change in Lepidoptera that has been objectively quantified and measured from the visual perspective of natural predators.

Colour change of twig-mimicking peppered moth larvae is a continuous reaction norm that increases camouflage against avian predators.

Science.gov (United States)

Eacock, Amy; Rowland, Hannah M; Edmonds, Nicola; Saccheri, Ilik J

2017-01-01

Camouflage, and in particular background-matching, is one of the most common anti-predator strategies observed in nature. Animals can improve their match to the colour/pattern of their surroundings through background selection, and/or by plastic colour change. Colour change can occur rapidly (a few seconds), or it may be slow, taking hours to days. Many studies have explored the cues and mechanisms behind rapid colour change, but there is a considerable lack of information about slow colour change in the context of predation: the cues that initiate it, and the range of phenotypes that are produced. Here we show that peppered moth ( Biston betularia ) larvae respond to colour and luminance of the twigs they rest on, and exhibit a continuous reaction norm of phenotypes. When presented with a heterogeneous environment of mixed twig colours, individual larvae specialise crypsis towards one colour rather than developing an intermediate colour. Flexible colour change in this species has likely evolved in association with wind dispersal and polyphagy, which result in caterpillars settling and feeding in a diverse range of visual environments. This is the first example of visually induced slow colour change in Lepidoptera that has been objectively quantified and measured from the visual perspective of natural predators.

76 FR 1338 - Emerald Ash Borer; Quarantined Areas; Maryland, Michigan, Minnesota, Missouri, Pennsylvania...

Science.gov (United States)

2011-01-10

... DEPARTMENT OF AGRICULTURE Animal and Plant Health Inspection Service 7 CFR Part 301 [Docket No. APHIS-2008-0072] Emerald Ash Borer; Quarantined Areas; Maryland, Michigan, Minnesota, Missouri..., Japan, Mongolia, the Russian Far East, Taiwan, and Canada, eventually kills healthy ash trees after it...

Influence of Binasal and Uninasal Inhalations of Essential Oil of Abies koreana Twigs on Electroencephalographic Activity of Human

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Min Seo

2016-01-01

Full Text Available Objectives. The present work investigates the effect of essential oil from the twigs of Abies koreana on electroencephalographic (EEG activity of human brain in order to understand the influence of binasal and uninasal inhalations. Methods. To accomplish this study, the essential oil from the twigs of A. koreana (AEO was isolated by steam distillation and the EEG readings were recorded using QEEG-8 system from 8 grounding electrodes according to the International 10-20 System. Results. D-Limonene (25.29%, bornyl acetate (19.31%, camphene (12.48%, α-pinene (11.88%, β-pinene (6.45%, and eudesm-7(11-en-ol (5.38% were the major components in the essential oil. In the EEG study, the absolute alpha (left frontal and right parietal and absolute fast alpha (right parietal values significantly increased during the binasal inhalation of AEO. In the uninasal inhalation, absolute beta and theta values decreased significantly, especially in the right frontal and left and right parietal regions. The results revealed that the AEO produced different EEG power spectrum changes according to the nostril difference. Conclusion. The changes in EEG values due to the inhalation of AEO may contribute to the enhancement of relaxation (binasal inhalation and alertness/attention (right uninasal inhalation states of brain which could be used in aromatherapy treatments.

Emerald ash borer impacts on visual preferences for urban forest recreation settings

Science.gov (United States)

Arne Arnberger; Ingrid E. Schneider; Martin Ebenberger; Renate Eder; Robert C. Venette; Stephanie A. Snyder; Paul H. Gobster; Ami Choi; Stuart Cottrell

2017-01-01

Extensive outbreaks of the emerald ash borer (Agrilus planipennis; EAB), an invasive forest insect, are having serious impacts on the cultural ecosystem services of urban forests in the United States and other countries. Limited experience with how such outbreaks might affect recreational opportunities prompted this investigation of visitors to a...

Interdisciplinary investigation on ancient Ephedra twigs from Gumugou Cemetery (3800 B.P.) in Xinjiang region, northwest China.

Science.gov (United States)

Xie, Mingsi; Yang, Yimin; Wang, Binghua; Wang, Changsui

2013-07-01

In the dry northern temperate regions of the northern hemisphere, the genus Ephedra comprises a series of native shrub species with a cumulative application history reaching back well over 2,000 years for the treatment of asthma, cold, fever, as well as many respiratory system diseases, especially in China. There are ethnological and philological evidences of Ephedra worship and utilization in many Eurasia Steppe cultures. However, no scientifically verifiable, ancient physical proof has yet been provided for any species in this genus. This study reports the palaeobotanical finding of Ephedra twigs discovered from burials of the Gumugou archaeological site, and ancient community graveyard, dated around 3800 BP, in Lop Nor region of northwestern China. The macro-remains were first examined by scanning electron microscope (SEM) and then by gas chromatography-mass spectrometry (GC-MS) for traits of residual biomarkers under the reference of modern Ephedra samples. The GC-MS result of chemical analysis presents the existence of Ephedra-featured compounds, several of which, including benzaldehyde, tetramethyl-pyrazine, and phenmetrazine, are found in the chromatograph of both the ancient and modern sample. These results confirm that the discovered plant remains are Ephedra twigs. Although there is no direct archaeological evidence for the indication of medicinal use of this Ephedra, the unified burial deposit in which the Ephedra was discovered is a strong indication of the religious and medicinal awareness of the human inhabitants of Gumugou towards this plant. Copyright © 2013 Wiley Periodicals, Inc.

Parasitoids attacking emerald ash borers in western Pennsylvania and their potential use in biological control

Science.gov (United States)

J.J. Duan; R.W. Fuester; J. Wildonger; P.B. Taylor; S. Barth; S-E. Spichiger

2009-01-01

Current biological control programs against the emerald ash borer (EAB, Agrilus planipennis Fairmaire) have primarily focused on the introduction and releases of exotic parasitoids from China, home of the pest origin....

Visualizing the mesothoracic spiracles in a bark beetle: The coffee berry borer, Hypothenemus hampei

Science.gov (United States)

In a low-temperature scanning electron microscopy study aimed at determining whether the coffee berry borer (Hypothenemus hampei (Ferrari); Coleoptera: Curculionidae: Scolytinae) possesses mycangia, we fortuitously detected the mesothoracic spiracles, which are usually concealed. The mesothoracic s...

Summary of core damage frequency from internal initiators: Peach Bottom

International Nuclear Information System (INIS)

Kolaczkowski, A.M.; Lambright, J.A.; Cathey, N.

1986-01-01

Probabilistic risk assessments (PRA) based on internal initiators are being conducted on a number of reference plants in order to provide the Nuclear Regulatory Commission (NRC) with updated information about light water reactor risk. The results of these analyses will be used by the NRC to prepare NUREG-1150 which will examine the NRC's current perception of risk. Peach Bottom has been chosen as one of the reference plants

Evaluation of recovery and monitoring methods for parasitoids released against Emerald Ash Borer

Science.gov (United States)

The emerald ash borer (Agrilus planipennis Fairmaire, EAB) is an invasive insect pest, and the target of an extensive biological control campaign designed to mitigate EAB driven ash tree (Fraxinus spp.) mortality. Since 2007, environmental releases of three species of hymenopteran parasitoids of EA...

Intraspecific variation in Fraxinus pennsylvanica responses to emerald ash borer (Agrilus planipennis)

Science.gov (United States)

J.L. Koch; D.W. Carey; M.E. Mason; T.M. Poland; K.S. Knight

2015-01-01

The emerald ash borer (EAB; Agrilus planipennis Fairmaire) is a bark and wood boring beetle native to east Asia that was first discovered in North America in 2002. Since then, entire stands of highly susceptible green ash (Fraxinus pennsylvanica Marshall) have been killed within a few years of infestation. We have identified a...

RELATIONSHIP BETWEEN EUROPEAN CORN BORER FEEDING ACTIVITY AND NITROGEN LEAF CONTENT UNDER DIFFERENT AGRICULTURAL PRACTICES

Directory of Open Access Journals (Sweden)

Ankica Sarajlić

2015-06-01

Full Text Available One of the most destructive maize pest in Croatia is European corn borer (Ostrinia nubilalis Hübner (ECB. The aim of this study was to determine the influence of irrigation, nitrogen fertilization, different maize genotypes and nitrogen leaf content on ECB feeding activity. The experiment was set up in Osijek, Croatia under field conditions during 2012-2013 vegetation season. Experiment treatments were as follows: three irrigation levels (A1 - control, A2 from 60% to 80% field water capacity - FWC and A3 from 80% to100% FWC, three nitrogen fertilizer levels (B1 - 0, B2 - 100 and B3 - 200 kg N/ha and four different genotypes (C1 - OSSK 596; C2 - OSSK 617; C3 - OSSK 602 and C4 - OSSK 552. Ear weight, number of larvae in stem and shank, tunnel length and nitrogen leaf content were evaluated. Genotype C1 was the most susceptible for following the tested variables of ECB feeding: tunnel length (TL, larvae in stalk (LS and total number of larvae (TNL at P<0.05 probability level. By raising the level of irrigation, European corn borer feeding activity was reduced while by raising the level of nitrogen fertilization feeding activity was increased. These results suggest that good production practices can significantly affect the susceptibility of maize to European corn borer.

Incidence of Infestation and Larval Success of Emerald Ash Borer (Agrilus planipennis) on White Fringetree (Chionanthus virginicus), Chinese Fringetree (Chionanthus retusus), and Devilwood (Osmanthus americanus).

Science.gov (United States)

Cipollini, Don; Rigsby, Chad M

2015-10-01

We compared the incidence of infestation by emerald ash borer (EAB) and lilac borer on white fringetree to that of its Asian congener, Chinese fringetree, Chionanthus retusus, and a North American relative, devilwood, Osmanthus americanus. We also conducted laboratory bioassays to determine the suitability of these hosts for EAB larvae. At Spring Grove Cemetery and Arboretum in Cincinnati, Ohio, 9 of 28 white fringetrees examined were infested by EAB. Most of the white fringetrees had lilac borer infestation, and most of the trees infested by EAB also had lilac borer infestation. None of the 11 Chinese fringetrees examined were infested by either EAB or lilac borer. Each of the five devilwood individuals examined was infested by lilac borer, but not EAB. At The Morton Arboretum in Lisle, Illinois, 7 of 16 white fringetrees examined were infested by EAB, while none of the seven Chinese fringetrees examined were infested by either insect. A 40-d bioassay confirmed that white fringetree was an acceptable host, producing fourth-instar larvae that were smaller than those produced on a highly susceptible cultivar of green ash, Fraxinus pennsylvanica. No larvae survived on Chinese fringetree, and neonates were largely incapable of feeding on it. Two larvae survived on devilwood, reaching the second instar and excavating extensive galleries. Future work should be aimed at biotic and abiotic factors influencing the susceptibility of white fringetree, as well as further examination of close relatives for their vulnerability to EAB. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Operating history report for the Peach Bottom HTGR. Volume I. Reactor operating history

International Nuclear Information System (INIS)

Scheffel, W.J.; Baldwin, N.L.; Tomlin, R.W.

1976-01-01

The operating history for the Peach Bottom-1 Reactor is presented for the years 1966 through 1975. Information concerning general chemistry data, general physics data, location of sensing elements in the primary helium circuit, and postirradiation examination and testing of reactor components is presented

Decontamination effects of bark washing with a high-pressure washer on Peach [Prunus persica (L.) Batsch] and Japanese Persimmon (Diospyros kaki Thunb.) contaminated with radiocaesium during dormancy

International Nuclear Information System (INIS)

Sato, Mamoru; Abe, Kazuhiro; Kikunaga, Hidetoshi; Ohtsuki, Tsutomu; Takata, Daisuke; Tanoi, Keitaro; Muramatsu, Yasuyuki

2015-01-01

The effect of bark washing with a high-pressure washer on deciduous trees contaminated during dormancy by radiocaesium fallout derived from the Fukushima Daiichi Nuclear Power Plant accident was examined using peach [Prunus persica (L.) Batsch] and Japanese persimmon (Diospyros kaki Thunb.). Eighteen-year-old peach trees ('Akatsuki') were bark-washed twice with a high-pressure washer on July 5 and 27, 2011. Seven-year-old peach trees ('Kawanakajima Hakuto') were bark-washed on January 24, 2012, and thirty-year-old Japanese persimmon trees ('Hachiya') were bark-washed on December 21, 2011. For the peach trees, most of the bark was not removed by washing with a high-pressure washer. In contrast, the rough bark of Japanese persimmon was removed completely. No significant differences in the 137 Cs concentration of 'Akatsuki' fruit were found between the treatments conducted in the summer of 2011. Upon the bark washing of peach 'Akatsuki' trees in summer, the possibility of secondary contamination of leaves via the leachate containing 137 Cs was likely. The 137 Cs concentrations in fruits and leaves of peach 'Kawanakajima Hakuto' collected in summer 2012 were decreased significantly by washing treatment conducted in winter 2011–2012. In the year after treatment, the 137 Cs concentrations in fruits and leaves of Japanese persimmon were significantly decreased by the treatment. The effect of the bark washing on decreasing 137 Cs contents in fruits and leaves was greater in Japanese persimmon than in peach. The results for 'Kawanakajima Hakuto' and 'Hachiya' demonstrated the possibility of additive contamination. (author)

Testing public Bt maize events for control of stem borers in the first ...

African Journals Online (AJOL)

Transgenic maize (Zea mays L), developed using modified genes from the bacterium Bacillus thuringiensis (Bt), controls stem borers without observable negative effects to humans, livestock or the environment, and is now sown on 134 million hectares globally. Bt maize could contribute to increasing maize production in ...

Survey for tolerance to emerald ash borer within North American ash species

Science.gov (United States)

Jennifer L. Koch; Mary E. Mason; David W. Carey; Kathleen Knight; Therese Poland; Daniel A. Herms

2010-01-01

Since the discovery of the emerald ash borer (EAB) near Detroit, MI, in 2002, more than 40 million ash trees have been killed and another 7.5 billion are at risk in the United States. When the EAB outbreak was initially discovered, our native ash species appeared to have no resistance to the pest.

Fermentation Characteristics and Lactic Acid Bacteria Succession of Total Mixed Ration Silages Formulated with Peach Pomace

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Xiaodong Hu

2015-04-01

Full Text Available The objective of this study was to assess the use of peach pomace in total mixed ration (TMR silages and clarify the differences in aerobic stability between TMR and TMR silages caused by lactic acid bacteria (LAB. The TMR were prepared using peach pomace, alfalfa hay or Leymus chinensis hay, maize meal, soybean meal, cotton meal, limestone, a vitamin-mineral supplement, and salt in a ratio of 6.0:34.0:44.4:7.0:5.0:2.5:1.0:0.1 on a dry matter (DM basis. Fermentation quality, microbial composition, and the predominant LAB were examined during ensiling and aerobic deterioration. The results indicated that the TMR silages with peach pomace were well fermented, with low pH and high lactic acid concentrations. The aerobic stability of TMR silages were significantly higher than that of TMR. Compared with TMR silages with alfalfa hay, TMR silage with Leymus chinensis hay was much more prone to deterioration. Although the dominant LAB were not identical in TMR, the same dominant species, Lactobacillus buchneri and Pediococcus acidilactici, were found in both types of TMR silages after 56 d of ensiling, and they may play an important role in the aerobic stability of TMR silages.

An integrated approach for increasing breeding efficiency in apple and peach in Europe

NARCIS (Netherlands)

Laurens, Francois; Aranzana, Maria José; Arus, Pere; Bassi, Daniele; Bink, Marco; Bonany, Joan; Caprera, Andrea; Corelli-Grappadelli, Luca; Costes, Evelyne; Durel, Charles Eric; Mauroux, Jehan Baptiste; Muranty, Hélène; Nazzicari, Nelson; Pascal, Thierry; Patocchi, Andrea; Peil, Andreas; Quilot-Turion, Bénédicte; Rossini, Laura; Stella, Alessandra; Troggio, Michela; Velasco, Riccardo; De Weg, Van Eric

2018-01-01

Despite the availability of whole genome sequences of apple and peach, there has been a considerable gap between genomics and breeding. To bridge the gap, the European Union funded the FruitBreedomics project (March 2011 to August 2015) involving 28 research institutes and private companies. Three

Effects of long-term consumption of high fructose corn syrup containing peach nectar on body weight gain in sprague dawley rats

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Gulsah OZCAN SINIR

Full Text Available Abstract High fructose corn syrup (HFCS is one of the most used sweeteners in the food industry. Health concerns regarding the consumption of HFCS-containing foods have developed in parallel with the increasing amount of people who become overweight. This study was conducted to investigate whether HFCS-containing peach nectar (pn-HFCS consumption has more detrimental effects on anthropometrical and biochemical parameters compared with sucrose-containing peach nectar (pn-sucrose. Fifty-day-old Sprague Dawley rats were divided into three groups and were fed (A pn-HFCS + ad libitum chow, (B pn-sucrose + ad libitum chow and (C only ad libitum chow for 7 months. The percentage change in body weight (PCBW, body mass index (BMI, and Lee index were calculated, and serum triglyceride, glucose, insulin and leptin concentrations were measured. The PCBW, BMI, Lee index, serum triglyceride, glucose, insulin and leptin concentrations were insignificant among the three groups. We can suggest that peach nectar consumption resulted in more energy intake than the control and since pn-HFCS group consumed more chow than the pn-sucrose group. The results show that long term daily HFCS or sucrose consumption in peach nectar is not associated with weight gain and does not stimulate metabolic changes in Sprague Dawley rats.

Microbiological analysis of peach palm in natura submitted to {sup 60}Co radiation

Energy Technology Data Exchange (ETDEWEB)

Silva, Priscila V.; Araujo, Michel M.; Nunes, Thaise C.F.; Costa, Helbert S.F.; Villavicencio, Anna Lucia C.H. [Instituto de Pesquisas Energeticas e Nucleares (IPEN-CNEN/SP), Sao Paulo, SP (Brazil)], e-mail: priscilavsilva@gmail.com, e-mail: villavic@ipen.br; Hojeije, Khalil Y. [Floresta Industria e Comercio Ltda., Sao Paulo, SP (Brazil)

2009-07-01

The palm tree (Bactris gasipaes Kunth) is a species with high potential benefits, because of the nutritional value of its fruits that could be used both in human and animals feeding and mainly for peach palm extraction. It represents a great source of dietary fiber and a moderate source of magnesium and iron. Food irradiation is a worldwide technology that aims to improve the product quality, in order to eliminate diverse microorganisms that can spoil the food. Radiation processing, in the recommended doses, causes very few chemical alterations and nutritional losses in foods, being considered insignificant and/or similar to other food treatments. The objective of this study was to evaluate the effect of irradiation on microbiological counts of mesophilic aerobic in the peach palm in natura. Samples were irradiated with 1.0 and 1.5 kGy using a {sup 60}Co multipurpose irradiator. Radiation treatment appeared to be a useful alternative to reduce microbial contamination in the samples analyzed. (author)

Factors that influence emerald ash borer (Agrilus planipennis) adult longevity and oviposition under laboratory conditions

Science.gov (United States)

Melody A. Keena; Juli Gould; Leah S. Bauer

2009-01-01

The emerald ash borer (EAB), Agrilus planipennis, is a nonnative insect from Asia that threatens ash trees in the urban and natural forests of North America. Research on this invasive insect and rearing parasitoids for...

[Evaluation of consumer's acceptance of a peach palm snack (Bactris gasipaes) and determination of its potential as a functional food].

Science.gov (United States)

López-Calvo, Rebeca; Pérez, Ana M; Ivankovich Guillén, Carmen; Calderón Villaplana, Sandra; Pineda Castro, Maria Lourdes

2015-03-01

The aim of this study was to evaluate consumers' acceptance of a peach palm snack and to determine its potential as a functional food by chemical characterization. An assessment was conducted with 100 consumers to determine the acceptance of different snack formulations and the results were subjected to cluster analysis. This analysis revealed two groups. Group 2 included people that consume snacks and peach palm frequently and showed the highest grades for the snack evaluated characteristics. All the consumers in group 2 and approximately 85% of the consumers in group 1 indicated that they would buy the product suggesting that there is a niche market for the developed peach palm snack. Also, a qualitative evaluation, using mini focus groups, of the two most widely accepted formulas of the snack (chosen according to previously described study) was performed. The sessions considered the opinion of middle class professionals and housewives. It was determined that the combination of tara gum and carboxymethylcellulose (CMC) allows a positive synergistic effect on the sensory characteristics of the snack, highlighting natural peach flavor and improving crunchiness. In a dry basis, the snack contains per 100 g: 9 ± 4 g of fat, 14.0 ± 0.3 g of dietary fiber, 15500 ± 32 µg of carotenoids and has an antioxidant capacity of 4700 ± 8 µmol TE, which demonstrates its potential as a functional food.

Fate of Escherichia coli O157:H7, Salmonella and Listeria innocua on minimally-processed peaches under different storage conditions.

Science.gov (United States)

Alegre, Isabel; Abadias, Maribel; Anguera, Marina; Usall, Josep; Viñas, Inmaculada

2010-10-01

Consumption of fresh-cut produce has sharply increased recently causing an increase of foodborne illnesses associated with these products. As generally, acidic fruits are considered 'safe' from a microbiological point of view, the aim of this work was to study the growth and survival of Escherichia coli O157:H7, Salmonella and Listeria innocua on minimally-processed peaches. The three foodborne pathogens population increased more than 2 log(10)units on fresh-cut peach when stored at 20 and 25 degrees C after 48 h. At 10 degrees C only L. innocua grew more than 1 log(10)unit and it was the only pathogen able to grow at 5 degrees C. Differences in growth occurred between different peach varieties tested, with higher population increases in those varieties with higher pH ('Royal Glory' 4.73+/-0.25 and 'Diana' 4.12+/-0.18). The use of common strategies on extending shelf life of fresh-cut produce, as modified atmosphere packaging and the use of the antioxidant substance, ascorbic acid (2%w/v), did not affect pathogens' growth at any of the temperatures tested (5 and 25 degrees C). Minimally-processed peaches have shown to be a good substrate for foodborne pathogens' growth regardless use of modified atmosphere and ascorbic acid. Therefore, maintaining cold chain and avoiding contamination is highly necessary. 2010 Elsevier Ltd. All rights reserved.

Quercivorol as a lure for the polyphagous and Kuroshio shot hole borers, Euwallacea spp. nr. fornicatus (Coleoptera: Scolytinae, vectors of Fusarium dieback

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Christine Dodge

2017-08-01

Full Text Available The polyphagous shot hole borer and Kuroshio shot hole borer, two members of the Euwallacea fornicatus species complex (Coleoptera: Curculionidae: Scolytinae, are invasive ambrosia beetles that harbor distinct species of Fusarium fungal symbionts. Together with the damage caused by gallery construction, these two phytopathogenic Fusarium species are responsible for the emerging tree disease Fusarium dieback, which affects over 50 common tree species in Southern California. Host trees suffer branch dieback as the xylem is blocked by invading beetles and fungi, forcing the costly removal of dead and dying trees in urban areas. The beetles are also threatening natural riparian habitats, and avocado is susceptible to Fusarium dieback as well, resulting in damage to the avocado industries in California and Israel. Currently there are no adequate control mechanisms for shot hole borers. This paper summarizes efforts to find a suitable lure to monitor shot hole borer invasions and dispersal. Field trials were conducted in two counties in Southern California over a span of two years. We find that the chemical quercivorol is highly attractive to these beetles, and perform subsequent field experiments attempting to optimize this lure. We also explore other methods of increasing trap catch and effects of other potential attractants, as well as the deterrents verbenone and piperitone.

Analysis of Peach Bottom turbine trip tests

International Nuclear Information System (INIS)

Cheng, H.S.; Lu, M.S.; Hsu, C.J.; Shier, W.G.; Diamond, D.J.; Levine, M.M.; Odar, F.

1979-01-01

Current interest in the analysis of turbine trip transients has been generated by the recent tests performed at the Peach Bottom (Unit 2) reactor. Three tests, simulating turbine trip transients, were performed at different initial power and coolant flow conditions. The data from these tests provide considerable information to aid qualification of computer codes that are currently used in BWR design analysis. The results are presented of an analysis of a turbine trip transient using the RELAP-3B and the BNL-TWIGL computer codes. Specific results are provided comparing the calculated reactor power and system pressures with the test data. Excellent agreement for all three test transients is evident from the comparisons

Progress and future directions in research on the emerald ash borer

Science.gov (United States)

Therese M. Poland

2014-01-01

When the emerald ash borer (EAB) was discovered near Detroit, Michigan in July 2002, very little was known about it other than the fact that it was killing large numbers of ash trees throughout a widespread area in southeast Michigan (Poland and McCullough 2006). Ash mortality in the area had been noted for a few years, but was attributed to ash decline until damage...

Laboratory Evaluation of the Toxicity of Systemic Insecticides to Emerald Ash Borer Larvae.

Science.gov (United States)

Poland, Therese M; Ciaramitaro, Tina M; McCullough, Deborah G

2016-04-01

Emerald ash borer (Agrilus planipennis Fairmaire) (Coleoptera: Buprestidae), an invasive phloem-feeding insect native to Asia, threatens at least 16 North American ash (Fraxinus) species and has killed hundreds of millions of ash trees in landscapes and forests. We conducted laboratory bioassays to assess the relative efficacy of systemic insecticides to control emerald ash borer larvae in winter 2009 and 2010. Second- and third-instar larvae were reared on artificial diet treated with varying doses of emamectin benzoate (TREE-äge, Arborjet, Inc., Woburn, MA), imidacloprid (Imicide, J. J Mauget Co., Arcadia, CA), dinotefuran (Safari, Valent Professional Products, Walnut Creek, CA), and azadirachtin (TreeAzin, BioForest Technologies, Inc., Sault Ste. Marie, Ontario, and Azasol, Arborjet, Inc., Woburn, MA). All of the insecticides were toxic to emerald ash borer larvae, but lethal concentrations needed to kill 50% of the larvae (LC50), standardized by larval weight, varied with insecticide and time. On the earliest date with a significant fit of the probit model, LC50 values were 0.024 ppm/g at day 29 for TREE-äge, 0.015 ppm/g at day 63 for Imicide, 0.030 ppm/g at day 46 for Safari, 0.025 ppm/g at day 24 for TreeAzin, and 0.027 ppm/g at day 27 for Azasol. The median lethal time to kill 50% (LT50) of the tested larvae also varied with insecticide product and dose, and was longer for Imicide and Safari than for TREE-äge or the azadirachtin products. Insecticide efficacy in the field will depend on adult and larval mortality as well as leaf and phloem insecticide residues.

Anti-inflammatory activities of compounds from twigs of Morus alba.

Science.gov (United States)

Tran, Huynh Nguyen Khanh; Nguyen, Van Thu; Kim, Jeong Ah; Rho, Seong Soo; Woo, Mi Hee; Choi, Jae Sui; Lee, Jeong-Hyung; Min, Byung Sun

2017-07-01

Five new compounds, 10-oxomornigrol F (1), (7″R)-(-)-6-(7″-hydroxy-3″,8″-dimethyl-2″,8″-octadien-1″-yl)apigenin (2), ramumorin A (3), ramumorin B (4), and (4S,7S,8R)-trihydroxyoctadeca-5Z-enoic acid (5), together with 31 known compounds (6-36), were isolated from the twigs of Morus alba (Moraceae). The chemical structures of these compounds were established using spectroscopic analyses, 1D and 2D NMR, high-resolution electrospray ionization mass spectrometry (HRESIMS), and Mosher's methods. The anti-inflammatory activities of the compounds were evaluated by investigating their ability to inhibit lipopolysaccharide (LPS)-induced nitric oxide (NO) production in macrophage RAW 264.7 cells. Compounds 1, 2, 13, 17, 19, 25-28, and 32 showed inhibitory effects with IC 50 values ranging from 2.2 to 5.3μg/mL. Compounds 1, 2, 17, 25, and 32 reduced LPS-induced inducible nitric oxide synthase (iNOS) expression in a concentration-dependent manner. In addition, pretreating the cells with compound 1, 17, and 32 significantly suppressed LPS-induced expression of cyclooxygenase-2 (COX-2) protein. Copyright © 2017. Published by Elsevier B.V.

Study on the viability of peach and apple pollen treated with gamma rays 60Co

International Nuclear Information System (INIS)

Filev, K.; Angelov, A.

1976-01-01

Pollen from Marygold peach and from Golden Delicious apple varieties was treated with gamma-rays at rates of 0.5, 1.5, 10.0, 50.0, 100, 200, 300, 400 and 500 kR, the irradiation intensity being 1350 r/min. Results point to a different influence of gamma-rays irradiation on the pollen germination. Apple pollen proved more radiosensitive. Least dose of o.5 kR exerted a stimulative effect in the pollen germination. As the irradiation dose rcse, the germination percentage declined. Doses of over 400 kR destroyed completely pollen viability in both fruit species. A linear semi-logarithmical dependence was ascertained between the irradiation rate and pollen germination. 5 and 10 kR proved most suitable for the purposes of the experimental mutagenesis at peach and apple pollen irradiation. (author)

Spinosad and the tomato borer Tuta absoluta: a bioinsecticide, an invasive pest threat, and high insecticide resistance.

Science.gov (United States)

Campos, Mateus R; Rodrigues, Agna Rita S; Silva, Wellington M; Silva, Tadeu Barbosa M; Silva, Vitória Regina F; Guedes, Raul Narciso C; Siqueira, Herbert Alvaro A

2014-01-01

The introduction of an agricultural pest species into a new environment is a potential threat to agroecosystems of the invaded area. The phytosanitary concern is even greater if the introduced pest's phenotype expresses traits that will impair the management of that species. The invasive tomato borer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), is one such species and the characterization of the insecticide resistance prevailing in the area of origin is important to guide management efforts in new areas of introduction. The spinosad is one the main insecticides currently used in Brazil for control of the tomato borer; Brazil is the likely source of the introduction of the tomato borer into Europe. For this reason, spinosad resistance in Brazilian populations of this species was characterized. Spinosad resistance has been reported in Brazilian field populations of this pest species, and one resistant population that was used in this study was subjected to an additional seven generations of selection for spinosad resistance reaching levels over 180,000-fold. Inheritance studies indicated that spinosad resistance is monogenic, incompletely recessive and autosomal with high heritability (h(2) = 0.71). Spinosad resistance was unstable without selection pressure with a negative rate of change in the resistance level ( = -0.51) indicating an associated adaptive cost. Esterases and cytochrome P450-dependent monooxygenases titration decreased with spinosad selection, indicating that these detoxification enzymes are not the underlying resistance mechanism. Furthermore, the cross-resistance spectrum was restricted to the insecticide spinetoram, another spinosyn, suggesting that altered target site may be the mechanism involved. Therefore, the suspension of spinosyn use against the tomato borer would be a useful component in spinosad resistance management for this species. Spinosad use against this species in introduced areas should be carefully monitored to

Transgenic rice plants expressing a fused protein of Cry1Ab/Vip3H has resistance to rice stem borers under laboratory and field conditions.

Science.gov (United States)

Chen, Yang; Tian, Jun-Ce; Shen, Zhi-Chen; Peng, Yu-Fa; Hu, Cui; Guo, Yu-Yuan; Ye, Gong-Yin

2010-08-01

Six transgenic rice, Oryza sativa L., lines (G6H1, G6H2, G6H3, G6H4, G6H5, and G6H6) expressing a fused Cry1Ab/Vip3H protein, were evaluated for resistance against the Asiatic rice borer, Chilo suppressalis (Walker) (Lepidoptera: Crambidae), and the stem borer Sesamia inferens (Walker) (Lepidoptera: Noctuidae) in the laboratory and field. The bioassay results indicated that the mortality of Asiatic rice borer and S. inferens neonate larvae on six transgenic lines from seedling to filling stage was up to 100% at 168 h after infestation. The cumulative feeding area by Asiatic rice borer neonate larvae on all transgenic lines was significantly reduced compared with the untransformed parental 'Xiushui 110' rice. A 2-yr field evaluation showed that damage during the vegetative stage (deadheart) or during the reproductive stage (whitehead) caused by Asiatic rice borer and S. inferens for transgenic lines was much lower than the control. For three lines (G6H1, G6H2, and G6H6), no damage was found during the entire growing period. Estimation of fused Cry1Ab/Vip3H protein concentrations using PathoScreen kit for Bt-Cry1Ab/1Ac protein indicated that the expression levels of Cry1Ab protein both in main stems (within the average range of 0.006-0.073% of total soluble protein) and their flag leaves (within the average range of 0.001-0.038% of total soluble protein) were significantly different among six transgenic lines at different developmental stages. Both laboratory and field researches suggested that the transgenic rice lines have considerable potential for protecting rice from attack by both stem borers.

Fission product behavior in the Peach Bottom and Fort St. Vrain HTGRs

International Nuclear Information System (INIS)

Hanson, D.L.; Baldwin, N.L.; Strong, D.E.

1980-11-01

Actual operating data from Peach Bottom and Fort St. Vrain were compared with code predictions to assess the validity of the methods used to predict the behavior of fission products in the primary coolant circuit. For both reactors the measured circuit activities were significantly below design values, and the observations generally verify the codes used for large HTGR design

Effect of Water Deficit Stress on Peach Growth under Commercial Orchard Management Conditions

Directory of Open Access Journals (Sweden)

M. Rahmati

2015-06-01

Full Text Available In order to study the sensitivity of vegetative growth to water deficit stress of a late-maturing peach (Prunus persica L. cv. Elberta under orchard conditions, an experiment was conducted as randomized complete-block design with three treatments and four repetitions in Shahdiran commercial orchard in Mashhad during 2011. Three irrigation treatments including 360 (low stress, 180 (moderate stress and 90 (severe stress m3ha-1week-1 using a drip irrigation system (minimum stem water potential near harvest: -1.2, -1.5 and -1.7 MPa, respectively from the mid-pit hardening stage (12th of June until harvest (23rd of Sep. applied. Predawn, stem and leaf water potentials, leaf photosynthesis, transpiration, stomatal conductance and leaf temperature, the number of new shoots on fruit bearing shoots and vegetative shoots lengths during growing season as well as leaf area at harvest were measured. The results showed that water deficit stress had negative effects on peach tree water status, thereby resulting in decreased leaf gas exchange and tree vegetative growth. As significant decreased assimilate production of tree was resulted from both decreased leaf assimilation rate (until about 23 % and 50 %, respectively under moderate and severe stress conditions compared to low stress conditions and decreased leaf area of tree (until about 57% and 79%, respectively under moderate and severe stress conditions compared to low stress conditions at harvest. The significant positive correlation between leaf water potential and vegetative growth of peach revealed that shoot growth would decrease by 30% and 50% of maximum at leaf water potential of –1.56 and –2.30 MPa, respectively.

[Effect of supplementation with peach palm as source of vitamin A: study with rats].

Science.gov (United States)

Yuyama, L K; Cozzolino, S M

1996-02-01

The effect of supplementation with peach palm (Bactris gasipaes H.B.K.), as an alternative source of vit. A, in the regional diet of Manaus, AM, Brazil, in which the pulp was cooked and transformed into flour, was studied. The biological trial involved rats which were depleted in zinc and vitamin A, followed by repletion using the regional diet (RD), RD plus peach palm and RD plus vitamin A. The parameters used to determine the utilization of vitamin A were the vitamin A concentrations in the liver and plasma, and the growth of the animals. The diet was prepared according to the data of Shrimpton and Giugliano for families earning less than two legal minimum salaries. Adult post-partum rats were used, with six male pups each, which received a diet based on casein washed with 1% EDTA, without the addition of zinc or vitamin A for a period of 25 days, for the purpose of obtaining newly-weaned animals which were deficient in Zn and Vit.A. A control group received a diet also based on casein washed with 1% EDTA, but with all the nutrients in the quantities suggested by the Committee on Laboratory Animal Diets. The repletion period of the newly-weaned rats was of 30 days and the experimental design was entirely randomized with four groups of eight rats each. The diet supplementation followed the recommendations of the Committee on Laboratory Animal Diets. At the end of the experiment, it was observed that rats which consumed the diet based on the regional diet of Manaus supplemented with either peach palm or vitamin A showed a significantly greater concentration of vitamin A in the liver, 43.3 +/- 6.5 micrograms/g, 42.0 +/- 4.3 micrograms/g, respectively in relation to the regional diet, 5.5 +/- 1.1 micrograms/g (p < 0.05). The amount of zinc present in the regional diet, 10.7 mg per day, was bioavailable as determined by the concentration of zinc in the femurs. The results suggest that the regional diet of Manaus needs to be supplemented with vitamin A to maintain the

Breeding strategies for the development of emerald ash borer - resistant North American ash

Science.gov (United States)

Jennifer L. Koch; David W. Carey; Kathleen S. Knight; Therese Poland; Daniel A. Herms; Mary E. Mason

2012-01-01

The emerald ash borer (Agrilus plannipennis; EAB) is a phloem-feeding beetle that is endemic to Asia. It was discovered in North America in 2002, found almost simultaneously near Detroit, Michigan and Windsor, Ontario, Canada. Adult beetles feed on ash (Fraxinus spp.) foliage, but larval feeding on phloem, cambium, and...

Analysis of the thermal monitoring data collected at the Peach Bottom Atomic Power Station

International Nuclear Information System (INIS)

Witten, A.J.; Gray, D.D.

1977-01-01

A comprehensive study of the data collected as part of the environmental technical specifications program for Units 2 and 3 of the Peach Bottom Atomic Power Station was conducted for the Office of Nuclear Regulatory Research of the U.S. Nuclear Regulatory Commission. The study included an analysis of both the hydrothermal and ecological data collected from 1967 through 1976. This paper presents the details of the hydrothermal analysis performed under this program. The two primary methods used for temperature monitoring, during both the preoperational and operational periods of the program, are a fixed thermograph network and boat survey measurements. Analysis of the boat survey data provides a fine resolution demonstrating variations in ambient temperature in Conowingo Pond, as well as providing a qualitative picture of the thermal plume produced by the Peach Bottom thermal discharge. The data from 18 thermograph stations was used for a quantitative probability analysis

Formulation of a peach ice cream as potential symbiotic food

OpenAIRE

VILLALVA, Fernando Josué; CRAVERO BRUNERI, Andrea Paula; VINDEROLA, Gabriel; GONÇALVEZ DE OLIVEIRA, Enzo; PAZ, Noelia Fernanda; RAMÓN, Adriana Noemí

2017-01-01

Abstract Today’s population increasingly demands and consumes healthy products. For this reason, the food industry has been developing and marketing food with added bioactive components. The aim of this work was to formulate a peach ice cream reduced in calories with an added probiotic (Bifidobacterium lactis Bb-12) and prebiotics (inulin), and to evaluate its sensory quality and acceptability as potential symbiotic food. The moisture content was 76.47%; 7.14% protein; 0.15% fat; 6.37%; carbo...

Characterization of the complete mitochondrial genome of Chilo auricilius and comparison with three other rice stem borers.

Science.gov (United States)

Cao, Shuang-Shuang; Du, Yu-Zhou

2014-09-15

The mitogenome of Chilo auricilius (Lepidoptera: Pyraloidea: Crambidae) was a circular molecule made up of 15,367 bp. Sesamia inferens, Chilo suppressalis, Tryporyza incertulas, and C. auricilius, are closely related, well known rice stem borers that are widely distributed in the main rice-growing regions of China. The gene order and orientation of all four stem borers were similar to that of other insect mitogenomes. Among the four stem borers, all AT contents were below 83%, while all AT contents of tRNA genes were above 80%. The genomes were compact, with only 121-257 bp of non-coding intergenic spacer. There are 56 or 62-bp overlapping nucleotides in Crambidae moths, but were only 25-bp overlapping nucleotides in the noctuid moth S. inferens. There was a conserved motif 'ATACTAAA' between trnS2 (UCN) and nad1 in Crambidae moths, but this same region was 'ATCATA' in the noctuid S. inferens. And there was a 6-bp motif 'ATGATAA' of overlapping nucleotides, which was conserved in Lepidoptera, and a 14-bp motif 'TAAGCTATTTAAAT' conserved in the three Crambidae moths (C. suppressalis, C. auricilius and T. incertulas), but not in the noctuid. Finally, there were no stem-and-loop structures in the two Chilo moths. Copyright © 2014 Elsevier B.V. All rights reserved.

Lethal trap trees: a potential option for emerald ash borer (Agrilus planipennis Fairmaire) management

Science.gov (United States)

Deborah G McCullough; Therese M. Poland; Phillip A. Lewis

2015-01-01

BACKGROUND: Economic and ecological impacts of ash (Fraxinus spp.) mortality resulting from emerald ash borer (EAB) (Agrilus planipennis Fairmaire) invasion are severe in forested, residential and urban areas. Management options include girdling ash trees to attract ovipositing adult beetles and then destroying infested trees...

Using Dutch elm disease-tolerant elm to restore floodplains impacted by emerald ash borer

Science.gov (United States)

Kathleen S. Knight; James M. Slavicek; Rachel Kappler; Elizabeth Pisarczyk; Bernadette Wiggin; Karen. Menard

2012-01-01

American elm (Ulmus Americana L.) was a dominant species in floodplains and swamps of the Midwest before Dutch elm disease (DED) (Ophiostoma ulmi and O.novo-ulmi) reduced its populations. In many areas, ash (Fraxinus spp.) became dominant in these ecosystems. Emerald ash borer (EAB) (...

Nutrient critical levels and availability in soils cultivated with peach palm (Bactris gasipaes Kunth. in Santo Domingo de Los Tsáchilas, Ecuador

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Carlos Julio Quezada Crespo

2017-04-01

Full Text Available Ecuador is the most important exporter of canned peach palm, however, to date ideal soil fertility characteristics for peach palm growers remain unknown. The aim of this research was to determine optimal levels of soil nutrients for the cultivation of peach palm, specifically with regards to soil cation exchange capacity in order to obtain higher yields. We worked with 20 farmsteads and their soils from the province of Santo Domingo de los Tsáchilas during the second half of 2014. Fields were evaluated based on a relative yield and extractable (modified Olsen nutrient contents in each soil were determined using regression modeling to determine critical levels of each nutrient and specifically to determine the ideal soil cation exchange capacity under peach palm cultivation. Our analysis established critical levels of soil pH (6.3; OM 6.5%; P 12.3 mg.dm-3; K 0.67 cmol.dm-3 K; Ca 5.1 cmol.dm-3 ; Mg 0.97 cmol.dm-3; and S 7.5 mg.dm-3. The ideal Ca: Mg: K soil cation exchange capacity was determined to be 76:14:10.

Response of Green Peach Aphids and Other Arthropods to Garlic Intercropped with Tobacco

NARCIS (Netherlands)

Lai, R.; You, M.; Lotz, L.A.P.; Vasseur, L.

2011-01-01

The green peach aphid, Myzus persicae (Sulzer), is an insect pest that causes extensive damage to tobacco (Nicotiana tabacum L.) in China. Field trials were conducted in 2008 and 2009 at Longyan in the Fujian Province (China) to evaluate the effects of garlic (Allium sativum L.) as a deterrent to

Phytoplasmas in apricot, peach and sour cherry orchards in East Bohemia, Czech Republic

Czech Academy of Sciences Publication Activity Database

Ludvíková, H.; Fránová, Jana; Suchá, J.

2011-01-01

Roč. 64, Suppl. (2011), s. 67-68 ISSN 1721-8861 R&D Projects: GA MŠk OC09021 Institutional research plan: CEZ:AV0Z50510513 Keywords : Candidatus Phytoplasma prunorum * PCR/RFLP * apricot, peach and sour cherry orchards Subject RIV: EE - Microbiology, Virology Impact factor: 0.592, year: 2011

Tree Stress and Mortality from Emerald Ash Borer Does Not Systematically Alter Short-Term Soil Carbon Flux in a Mixed Northeastern U.S. Forest

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Jaclyn Hatala Matthes

2018-01-01

Full Text Available Invasive insect pests are a common disturbance in temperate forests, but their effects on belowground processes in these ecosystems are poorly understood. This study examined how aboveground disturbance might impact short-term soil carbon flux in a forest impacted by emerald ash borer (Agrilus planipennis Fairmaire in central New Hampshire, USA. We anticipated changes to soil moisture and temperature resulting from tree mortality caused by emerald ash borer, with subsequent effects on rates of soil respiration and methane oxidation. We measured carbon dioxide emissions and methane uptake beneath trees before, during, and after infestation by emerald ash borer. In our study, emerald ash borer damage to nearby trees did not alter soil microclimate nor soil carbon fluxes. While surprising, the lack of change in soil microclimate conditions may have been a result of the sandy, well-drained soil in our study area and the diffuse spatial distribution of canopy ash trees and subsequent canopy light gaps after tree mortality. Overall, our results indicate that short-term changes in soil carbon flux following insect disturbances may be minimal, particularly in forests with well-drained soils and a mixed-species canopy.

A bulk segregant gene expression analysis of a peach population reveals components of the underlying mechanism of the fruit cold response.

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Clara Pons

Full Text Available Peach fruits subjected for long periods of cold storage are primed to develop chilling injury once fruits are shelf ripened at room temperature. Very little is known about the molecular changes occurring in fruits during cold exposure. To get some insight into this process a transcript profiling analyses was performed on fruits from a PopDG population segregating for chilling injury CI responses. A bulked segregant gene expression analysis based on groups of fruits showing extreme CI responses indicated that the transcriptome of peach fruits was modified already during cold storage consistently with eventual CI development. Most peach cold-responsive genes have orthologs in Arabidopsis that participate in cold acclimation and other stresses responses, while some of them showed expression patterns that differs in fruits according to their susceptibility to develop mealiness. Members of ICE1, CBF1/3 and HOS9 regulons seem to have a prominent role in differential cold responses between low and high sensitive fruits. In high sensitive fruits, an alternative cold response program is detected. This program is probably associated with dehydration/osmotic stress and regulated by ABA, auxins and ethylene. In addition, the observation that tolerant siblings showed a series of genes encoding for stress protective activities with higher expression both at harvest and during cold treatment, suggests that preprogrammed mechanisms could shape fruit ability to tolerate postharvest cold-induced stress. A number of genes differentially expressed were validated and extended to individual genotypes by medium-throughput RT-qPCR. Analyses presented here provide a global view of the responses of peach fruits to cold storage and highlights new peach genes that probably play important roles in the tolerance/sensitivity to cold storage. Our results provide a roadmap for further experiments and would help to develop new postharvest protocols and gene directed breeding

A bulk segregant gene expression analysis of a peach population reveals components of the underlying mechanism of the fruit cold response.

Science.gov (United States)

Pons, Clara; Martí, Cristina; Forment, Javier; Crisosto, Carlos H; Dandekar, Abhaya M; Granell, Antonio

2014-01-01

Peach fruits subjected for long periods of cold storage are primed to develop chilling injury once fruits are shelf ripened at room temperature. Very little is known about the molecular changes occurring in fruits during cold exposure. To get some insight into this process a transcript profiling analyses was performed on fruits from a PopDG population segregating for chilling injury CI responses. A bulked segregant gene expression analysis based on groups of fruits showing extreme CI responses indicated that the transcriptome of peach fruits was modified already during cold storage consistently with eventual CI development. Most peach cold-responsive genes have orthologs in Arabidopsis that participate in cold acclimation and other stresses responses, while some of them showed expression patterns that differs in fruits according to their susceptibility to develop mealiness. Members of ICE1, CBF1/3 and HOS9 regulons seem to have a prominent role in differential cold responses between low and high sensitive fruits. In high sensitive fruits, an alternative cold response program is detected. This program is probably associated with dehydration/osmotic stress and regulated by ABA, auxins and ethylene. In addition, the observation that tolerant siblings showed a series of genes encoding for stress protective activities with higher expression both at harvest and during cold treatment, suggests that preprogrammed mechanisms could shape fruit ability to tolerate postharvest cold-induced stress. A number of genes differentially expressed were validated and extended to individual genotypes by medium-throughput RT-qPCR. Analyses presented here provide a global view of the responses of peach fruits to cold storage and highlights new peach genes that probably play important roles in the tolerance/sensitivity to cold storage. Our results provide a roadmap for further experiments and would help to develop new postharvest protocols and gene directed breeding strategies to better

Definition of Linear Color Models in the RGB Vector Color Space to Detect Red Peaches in Orchard Images Taken under Natural Illumination

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Jordi Palacín

2012-06-01

Full Text Available This work proposes the detection of red peaches in orchard images based on the definition of different linear color models in the RGB vector color space. The classification and segmentation of the pixels of the image is then performed by comparing the color distance from each pixel to the different previously defined linear color models. The methodology proposed has been tested with images obtained in a real orchard under natural light. The peach variety in the orchard was the paraguayo (Prunus persica var. platycarpa peach with red skin. The segmentation results showed that the area of the red peaches in the images was detected with an average error of 11.6%; 19.7% in the case of bright illumination; 8.2% in the case of low illumination; 8.6% for occlusion up to 33%; 12.2% in the case of occlusion between 34 and 66%; and 23% for occlusion above 66%. Finally, a methodology was proposed to estimate the diameter of the fruits based on an ellipsoidal fitting. A first diameter was obtained by using all the contour pixels and a second diameter was obtained by rejecting some pixels of the contour. This approach enables a rough estimate of the fruit occlusion percentage range by comparing the two diameter estimates.

Illustrated guide to the emerald ash borer, Agrilus planipennis Fairmaire and related species (Coleoptera, Buprestidae)

Science.gov (United States)

The 33 species of Agrilus (Coleoptera: Buprestidae) hypothesized to be most closely related to Agrilus planipennis Fairmaire (the emerald ash borer), are described and illustrated. Morphology (adults and immatures), biology, distribution, detailed taxonomic history and systematics are presented fo...

Recent development and advances in survey and detection tools for emerald ash borer

Science.gov (United States)

Therese M. ​Poland; Deborah G. McCullough; Taylor Scarr; Joseph Francese; Damon Crook; Michael Domingue; Harold Thistle; Brian Strom; Laura Blackburn; Daniel A. Herms; Krista Ryall; Patrick. Tobin

2016-01-01

The emerald ash borer (EAB, Agrilus planipennis Fairmaire) has killed hundreds of millions of ash trees since it was discovered near Detroit, Michigan and Windsor, Ontario in 2002 (www.emeraldashborer. info 2016) and continues to spread in North America. Canadian and U.S. federal, provincial, and state regulatory agencies have used artificial traps...

An assessment of the relationship between emerald ash borer presence and landscape pattern

Science.gov (United States)

Susan J. Crocker; Dacia M. Meneguzzo

2009-01-01

Six years after its 2002 detection near Detroit, MI, the emerald ash borer (EAB) (Agrilus planipennis Fairmaire) has spread hundreds of miles across the Upper Midwest and Mid-Atlantic regions of the United States. Human-assisted transportation of infested ash materials is the primary mechanism of EAB dispersal over long distances. Natural spread...

Exploring the molecular and biochemical basis of ash resistance to emerald ash borer

Science.gov (United States)

Justin G.A. Whitehill; Daniel A. Herms; Pierluigi. Bonello

2010-01-01

Larvae of the emerald ash borer (EAB) (Agrilus planipennis) feed on phloem of ash (Fraxinus spp.) trees. It is hypothesized that the resistance of Asian species of ash (e.g., Manchurian ash, F. mandshurica) to EAB is due to endogenous defenses present in phloem tissues in the form of defensive proteins and/or...

Classification and Discrimination of Different Fungal Diseases of Three Infection Levels on Peaches Using Hyperspectral Reflectance Imaging Analysis

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Ye Sun

2018-04-01

Full Text Available Peaches are susceptible to infection from several postharvest diseases. In order to control disease and avoid potential health risks, it is important to identify suitable treatments for each disease type. In this study, the spectral and imaging information from hyperspectral reflectance (400~1000 nm was used to evaluate and classify three kinds of common peach disease. To reduce the large dimensionality of the hyperspectral imaging, principal component analysis (PCA was applied to analyse each wavelength image as a whole, and the first principal component was selected to extract the imaging features. A total of 54 parameters were extracted as imaging features for one sample. Three decayed stages (slight, moderate and severe decayed peaches were considered for classification by deep belief network (DBN and partial least squares discriminant analysis (PLSDA in this study. The results showed that the DBN model has better classification results than the classification accuracy of the PLSDA model. The DBN model based on integrated information (494 features showed the highest classification results for the three diseases, with accuracies of 82.5%, 92.5%, and 100% for slightly-decayed, moderately-decayed and severely-decayed samples, respectively. The successive projections algorithm (SPA was used to select the optimal features from the integrated information; then, six optimal features were selected from a total of 494 features to establish the simple model. The SPA-PLSDA model showed better results which were more feasible for industrial application. The results showed that the hyperspectral reflectance imaging technique is feasible for detecting different kinds of diseased peaches, especially at the moderately- and severely-decayed levels.

Carbohydrate metabolism of vegetative and reproductive sinks in the late-maturing peach cultivar 'Encore'

Science.gov (United States)

Riccardo Lo Bianco; Mark Rieger; Shi-Jean S. Sung

1999-01-01

Activities of NAD+-dependent sorbitol dehydrogenase (SDH), sorbitol oxidase (SOX), sucrose synthase (SS), acid invertase (AI), and neutral invertase (NI) in ?Encore? peach (Prunus persica L.) fruits and developing shoot tips were assayed during the growing season to determine whether carbohydrate metabolizing enzymes could...

DURABILTY OF 25 LOCAL SPECIFIC WOOD SPECIES FROM JAVA PRESERVED WITH CCB AGAINST MARINE BORERS ATTACK

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Mohammad Muslich

2010-12-01

Full Text Available This study was conducted to provide basis information of the 25 local specific wood species indigenous from Java treated by copper bichromated boron (CCB. The full-cell process for 2 hours and 150 psi during the pressure-keeping period was employed. The IUFRO method was applied for the determination of wood treatability class. The treated and untreated wood specimens were tied together using plastic cord, arranged into a raft like assembly, and then exposed for 3, 6, and 12 months to the brackish water situated at Rambut Island’s coastal area. The Nordic Wood Preservation Council (NWPC standard No.1.4.2.2/75 was used to determine the intensity of marine borer infestation. The results revealed that 19 out of those 25 species were classified as easy to be preser ved, four species as moderate, and the remaining two were difficult to be preser ved. Those 19 species, i.e. Tamarindus indica L., Diplodiscus sp., Ficus variegate R .Br., Ehretia acuminata R .Br., Meliocope lunu-ankenda (Gaertn T.G. Hartley, Colona javanica B.L., Pouteria duclitanBachni., Stercularia oblongata R .Br., Ficus vasculosa Wall ex Miq., Callophyllum grandiflorum JJS., Turpinia sphaerocarpa Hassk., Neolitsea triplinervia Merr., Acer niveum Bl., Sloanea sigun Szysz., Castanopsis acuminatissima A.DC., Cinnamomum iners Reinw. Ex Blume., Litsea angulata Bl., Ficus nervosa Heyne., and Horsfieldia glabra Warb. were more permeable implying that the CCB retention and penetration were greater and deeper. Hymeneaecarboril.L., LitseaodoriferaVal., Gironniera subasqualisPlanch., and LinderapolyanthaBoerl. were moderately permeable. Castanopsis tunggurut A.DC. and Azadirachta indica Juss. were the least permeable judging that the CCB retention and penetration were lowest and shallowest. The treated wood specimens in this regard were able to prevent marine borers attack. Meanwhile, the untreated specimens were susceptible to marine borers attack, except Azadirachta indica. The attacking

Screening Quality Evaluation Factors of Freeze-Dried Peach (Prunus Persica L. Batsch Powders from Different Ripening Time Cultivars

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Chun-ju Liu

2017-01-01

Full Text Available The quality evaluation of processed products is complex. To simplify the quality evaluation process and improve the efficiency, fourteen evaluation factors of freeze-dried powders of seventeen cultivars of peach at different ripening times were analyzed. The most important evaluation indicators and criteria were obtained by analysis of variance (ANOVA, correlation analysis (CA, principal component analysis (PCA, system cluster analysis (SCA, and analytic hierarchy process (AHP. Results showed that the peach powders had the significant differences in quality (P<0.05, and some processing factors were related with some physicochemical and nutritional factors. Five principle components were extracted by PCA and the cumulative contribution achieved was 84.46%. Through the score plot of the first two principal components, a clear differentiation among ripening times was found and three distinct groups were separated according to ripening time. Five characteristic factors were obtained as titratable acid, browning index, hemicellulose, hygroscopicity, and vitamin C by SCA. Their weights of 0.1249, 0.3007, 0.0514, 0.4916, and 0.0315 were obtained by AHP, respectively. The peach cultivars were divided into four evaluation grades by the comprehensive quality score.

Efeito do hidrorresfriamento na qualidade de pêssegos 'Chimarrita' e 'Chiripá' Effect of hydrocooling on the quality of 'Chimarrita' and 'Chiripá' peaches

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Eduardo Seibert

2007-08-01

Full Text Available No presente trabalho, foram avaliados os efeitos do hidrorresfriamento para manutenção da qualidade em pêssegos 'Chimarrita' e 'Chiripá'. O hidrorresfriamento foi realizado por imersão em água com gelo a 5ºC, sendo o abaixamento da temperatura monitorado por 3 minutos. O tratamento-controle foi transferência para a câmara fria imediatamente após a colheita dos pêssegos. Os frutos foram armazenados a 0ºC ('Chiripá' e 0,5ºC ('Chimarrita' por 7; 14 e 21 dias e avaliados na colheita, nas saídas de frio, seguidos de 2 a 3 dias a 20ºC para o amadurecimento. O hidrorresfriamento foi efetivo em retirar o calor de campo dos frutos. Os frutos hidrorresfriados desidrataram mais na armazenagem, e pêssegos de ambos os tratamentos apresentaram murchamento no amadurecimento. A ocorrência de podridões não diferiu entre pêssegos hidrorresfriados e controles no armazenamento, nas duas cultivares, mas foram maiores no tratamento-controle, no amadurecimento na 'Chimarrita'. A firmeza da polpa foi igual entre os tratamentos na 'Chimarrita' e superior nos pêssegos 'Chiripá' hidrorresfriados durante a armazenagem. No amadurecimento a 20°C, a firmeza de polpa de ambas as cultivares decresceu a valores semelhantes nos frutos-controle e nos hidrorresfriados. Após 21 dias em frio, 50% dos pêssegos da cv. Chiripá e 15% dos pêssegos da cv. Chimarrita, de ambos os tratamentos, apresentaram lanosidade. O distúrbio retenção de firmeza ocorreu em 20% dos pêssegos 'Chiripá' e em 50% dos 'Chimarrita'.In the present work the effects of hydro cooling on the quality of 'Chimarrita' and 'Chiripá' peaches were evaluated. Peaches were hydro cooled by immersion in water at 5ºC with flesh temperatures monitored for 3 minutes and thereafter stored at 0ºC ('Chiripá' or 0,5ºC ('Chimarrita' for 7, 14 or 21 days. Samples were evaluated at harvest, after retrieval from cold storage and after 2 or 3 more days at 20ºC for ripening. Hydrocooled peaches

Three-year progression of emerald ash borer-induced decline and mortality in southeastern Michigan

Science.gov (United States)

Kamal J.K. Gandhi; Annemarie Smith; Robert P. Long; Robin A.J. Taylor; Daniel A. Herms

2008-01-01

We monitored the progression of ash (Fraxinus spp.) decline and mortality due to emerald ash borer (EAB), Agrilus planipennis, in 38 forest stands in the upper Huron River watershed region of southeastern Michigan from 2004-2007. Black ash (F. nigra), green ash (F. pennsylvanica), and white ash...

Genome-wide identification of WRKY family genes in peach and analysis of WRKY expression during bud dormancy.

Science.gov (United States)

Chen, Min; Tan, Qiuping; Sun, Mingyue; Li, Dongmei; Fu, Xiling; Chen, Xiude; Xiao, Wei; Li, Ling; Gao, Dongsheng

2016-06-01

Bud dormancy in deciduous fruit trees is an important adaptive mechanism for their survival in cold climates. The WRKY genes participate in several developmental and physiological processes, including dormancy. However, the dormancy mechanisms of WRKY genes have not been studied in detail. We conducted a genome-wide analysis and identified 58 WRKY genes in peach. These putative genes were located on all eight chromosomes. In bioinformatics analyses, we compared the sequences of WRKY genes from peach, rice, and Arabidopsis. In a cluster analysis, the gene sequences formed three groups, of which group II was further divided into five subgroups. Gene structure was highly conserved within each group, especially in groups IId and III. Gene expression analyses by qRT-PCR showed that WRKY genes showed different expression patterns in peach buds during dormancy. The mean expression levels of six WRKY genes (Prupe.6G286000, Prupe.1G393000, Prupe.1G114800, Prupe.1G071400, Prupe.2G185100, and Prupe.2G307400) increased during endodormancy and decreased during ecodormancy, indicating that these six WRKY genes may play a role in dormancy in a perennial fruit tree. This information will be useful for selecting fruit trees with desirable dormancy characteristics or for manipulating dormancy in genetic engineering programs.

DNA-based identification of Armillaria isolates from peach orchards in Mexico state

Science.gov (United States)

Ruben Damian Elias Roman; Ned B. Klopfenstein; Dionicio Alvarado Rosales; Mee-Sook Kim; Anna E. Case; Sara M. Ashiglar; John W. Hanna; Amy L. Ross-Davis; Remigio A. Guzman Plazola

2012-01-01

A collaborative project between the Programa de Fitopatología, Colegio de Postgraduados, Texcoco, Estado de Mexico and the USDA Forest Service - RMRS, Moscow Forest Pathology Laboratory has begun this year (2011) to assess which species of Armillaria are causing widespread and severe damage to the peach orchards from México state, Mexico. We are employing a DNA-based...

Status and potential of F1 sterility for control of European corn borer, Ostrinia nubilalis Hb

International Nuclear Information System (INIS)

Rosca, I.; Barbulescu, A.

1994-01-01

In certain lepidopterous insects partially gamma-ray-sterilized males mated with normal females produce progeny which are more sterile than their male parents. Inherited sterility has been observed in numerous pests including the European corn borer, Ostrinia nubilalis Hb. The most important discoveries contributing the development of this techniques are reviewed. The studies on the European corn borer have revealed a dramatic inherited sterility effect when pupae have been irradiated with a low dose of 100 or 150 Gy. Data on the growth, development and behaviour of F 1 individuals indicate that the treated insects are highly competitive with the normal insects. Field tests of the inherited sterility technique in isolated O. nubilalis infestations have indicated that this method is effective and a small eradication has been done. These studies are continuing. (author)

Feeding and Development of Emerald Ash Borer (Coleoptera: Buprestidae) on Cultivated Olive, Olea europaea.

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Cipollini, Don; Rigsby, Chad M; Peterson, Donnie L

2017-08-01

We examined the suitability of cultivated olive, Olea europaea L., as a host for emerald ash borer, Agrilus planipennis Fairmaire. In a bioassay using cut stems from a field-grown olive tree (cv. 'Manzanilla') we found that 45% of larvae that had emerged from eggs used to inoculate stems, were recovered alive, many as larvae or prepupae, during periodic debarking of a subset of stems. Three intact stems that 19 larvae successfully entered were exposed to a simulated overwintering treatment. Four live adults emerged afterwards, and an additional pupa and several prepupae were discovered after debarking these stems. Cultivated olive joins white fringetree as one of the two species outside of the genus Fraxinus capable of supporting the development of emerald ash borer from neonate to adult. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Effects of gamma irradiation on different phases of coffee borer Hypothenemus hampei (Ferrari, 1867)

International Nuclear Information System (INIS)

Wiendl, F.M.; Silva, A.L. da.

1974-10-01

Two experiments carried out in order to determine immediate lethal doses (LD sub(I)) for gamma irradiation of larvae, pupae and adults hypothenemus hampei (Ferrari, 1867) are presented. One experiment aimed only the determination of LD sub(I) for the adults of the coffee borer-outside the coffee-berries. The other to obtain the equivalent data for insects inside the coffee-berry, for all phases of the development cycle of the insect. It was found that LD sub(I) for larvae was around 350 Krad and for pupae around 400 Krad. For the adults, the LD sub(I) for insects outside the coffee-berry was 475 Krad and 525 for insects inside the coffee-berry. It was found that smaller doses caused a pronunced decrease in the insect lifetime, lifetime decrease proportionally as the irradiation dose increase. According to the results obtained, is postulated that this species of coffee-borer may be considered resistant to gamma radiation [pt

Walnut twig beetle: update on the biology and chemical ecology of a vector of an invasive fatal disease of walnut in the western U.S.

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Steven J. Seybold; Andrew D. Graves; Tom W. Coleman

2011-01-01

The walnut twig beetle, Pityophthorus juglandis Blackman (Coleoptera: Scolytidae) (sensu Wood 2007), is a native North American bark beetle that has been recently implicated as the vector of thousand cankers disease of walnut trees in the western U.S. (Tisserat et al. 2009, Utley et al. 2009, Seybold et al. 2010).

Efficacy of insecticides in fruit borer control and residues on sugar apple fruit

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Alessandro da Silva Oliveira

Full Text Available ABSTRACT Bahia is the Brazilian state with the largest production of sugar apple fruits (Annona squamosa L., and fruit borer (Cerconota anonella, Sepp. 1830 is a key crop pest. Insecticides are the main strategy for pest control even though there are no pesticides registered for this crop. This study aimed to assess the efficacy of insecticides to control fruit borer and determine the levels of insecticide residues in sugar apple fruits aiming at requesting the extension of authorization to use insecticide products in this crop. The experiment was conducted in an eight-year-old irrigated orchard (2 × 4 m located in Anagé, Bahia, Brazil. The experimental design was a randomized block design with 10 treatments (three insecticides with three doses and a control with water and 5 replications. Each plot was composed of four plants but only the two central ones were assessed. Insecticides and doses (g a.i. 100 L−1 water were Bacillus thuringiensis: 0.8, 1.7, and 2.5; triflumuron: 2.4, 3.6, and 4.8; and imidacloprid: 4.0, 10.0, and 16.0. Nine sprayings were carried out at fortnightly intervals with a costal sprayer with constant pressure, JA-2 nozzle, and with jet directed to the fruits. Ten assessments were performed in order to observe fruit borer presence in 30 previously marked fruits per plot. Imidacloprid, at the highest studied dose, was the only effective treatment. Analyses of imidacloprid residues, at 21 and 30 days after the highest dose application, indicated levels higher than the maximum limit allowed. Insecticides under the conditions tested do not meet the norms for requesting the extension of authorization to use insecticides for citrus in sugar apple fruits.

Distribution of the root system of peach palm under drip irrigation

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Adriano da Silva Lopes

2014-07-01

Full Text Available The incorporation of technologies has resulted in increased productivity and the more rational management of peach palm, with irrigation being an important tool for certain regions. Thus, studies leading to proper crop management are extremely important, such as the estimate of the effective depth of the root system, which is indispensable for proper irrigation management. The objective of this study was to evaluate the effects of different irrigation depths, as applied by drip irrigation, on the distribution of the root system of peach palm. This experiment was conducted in Ilha Solteira, São Paulo State, Brazil, with drip irrigation, with the two systems (flow of 0.0023 m3 h-1 consisting of four irrigation treatments corresponding to 0, 50, 100 and 150% of Class ‘A’ pan evaporation. After five years, an analysis of the Bactris gasipaes root system was performed at a distance of 0.0, 0.5 and 1.0 meters from the trunk, collecting sampling at two depths (0.0 to 0.3 m and 0.3 to 0.6 m via the auger method (volumetric analysis. We concluded that the effective depth of the root system used for irrigation management should be a maximum of 0.3 meters.

Chitin enhances biocontrol of Rhodotorula mucilaginosa to postharvest decay of peaches.

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Zhang, Hongyin; Yang, Qiya; Ge, Lingling; Zhang, Guochao; Zhang, Xiaoli; Zhang, Xiaoyun

2016-07-01

Biological control using microbial antagonists is a promising alternative approach to synthetic fungicides. However, effective biological control requires enhancing the consistency and efficacy of the antagonists used to control postharvest diseases. This study investigated the effect of chitin on the biocontrol efficacy of Rhodotorula mucilaginosa against blue mold and Rhizopus decay of peaches and on the protein expression profiles of R. mucilaginosa. The antagonistic activity of R. mucilaginosa harvested from the nutrient yeast dextrose broth (NYDB) with 0.5% chitin added was significantly improved compared with culture in NYDB without chitin. The R. mucilaginosa population cultured in chitin-supplement NYDB and nutrient yeast chitin borth (NYCB) harvested from peach wounds was more than that of R. mucilaginosa cultured in NYDB without chitin throughout the storage period except at 1 d. The protein expression profiles findings revealed that there were several differentially expressed proteins of R. mucilaginosa in the 0.5% chitin-supplemented NYDB and NYCB compared with that of R. mucilaginosa in NYDB. Most of these were cellular proteomes relating to the primary metabolic reactions such as glycoside hydrolases, phosphoribosyl pyrophosphate, and NADH dehydrogenases. Some proteins were also related to signal transmission and stress response. Copyright © 2016 Elsevier B.V. All rights reserved.

Validation of the Engineering Plant Analyzer methodology with Peach Bottom 2 stability tests

International Nuclear Information System (INIS)

Rohatgi, U.S.; Mallen, A.N.; Cheng, H.S.; Wulff, W.

1994-01-01

The Engineering Plant Analyzer (EPA) had been developed in 1984 at Brookhaven National Laboratory to simulate plant transients in boiling water reactors (BWR). Recently, the EPA with its High-Speed Interactive Plant Analyzer code for BWRs ( ppercase HIPA-BWR ) simulated for the first time oscillatory transients with large, non-linear power and flow amplitudes; transients which are centered around the March 9, 1988 instability at the LaSalle-2 BWR power plant.The EPA's capability to simulate oscillatory transients has been demonstrated first by comparing simulation results with LaSalle-2 plant data (Wulff et al., NUREG/CR-5816, BNL-NUREG-52312, Brookhaven National Laboratory, 1992). This paper presents an EPA assessment on the basis of the Peach Bottom 2 instability tests (Carmichael and Niemi, EPRI NP-564, Electric Power Research Institute, Palo Alto, CA, 1978). This assessment of the EPA appears to constitute the first validation of a time-domain reactor systems code on the basis of frequency-domain criteria, namely power spectral density, gain and phase shift of the pressure-to-power transfer function.The reactor system pressure was disturbed in the Peach Bottom 2 power plant tests, and in their EPA simulation, by a pseudo-random, binary sequence signal. The data comparison revealed that the EPA predicted for Peach Bottom tests PT1, PT2, and PT4 the gain of the power-to-pressure transfer function with the biases and standard deviations of (-10±28)%, (-1±40)% and (+28±52)%, respectively. The respective frequencies at the peak gains were predicted with the errors of +6%, +3%, and -28%. The differences between the predicted and the measured phase shift increased with increasing frequency, but stayed within the margin of experimental uncertainty. ((orig.))

Prospects for long-term ash survival in the core emerald ash borer mortality zone

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Jordan M. Marshall; Andrew J. Storer; Roger Mech; Steven A. Katovich

2011-01-01

Attacking all North American ash species (Fraxinus spp.), emerald ash borer (EAB) (Agrilus planipennis Fairmaire) has caused significant mortality within its introduced range. For other forest pests, host bark plays an important role in infestation density and oviposition behavior. The objectives of this study were to (1) locate...

Reconstructing the temporal and spatial dynamics of emerald ash borer adults through dendrochronological analyses

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Nathan W. Siegert; Deborah G. McCullough; Andrew M. Liebhold; Frank W. Telewski

2007-01-01

Emerald ash borer (Agrilus planipennis Fairmaire; Coleoptera: Buprestidae) was identified in June 2002 as the cause of widespread ash (Fraxinus spp.) mortality in southeastern lower Michigan and Windsor, Ontario. Localized outlier populations have since been discovered across much of lower Michigan and in areas of Indiana, Ohio and...

Tolerance of different rice genotypes (oryza sativa l.) against the infestation of rice stem borers under natural field conditions

International Nuclear Information System (INIS)

Sarwar, M.; Ahmad, N.; Nasrullah; Tofique, M.

2010-01-01

The present studies report the genotypic responses of 61 rice (Oryza sativa L.) genotypes (35 aromatic and 26 non aromatic) against the infestation of rice stem borers under natural field conditions. The data obtained on these genotypes on larval infestation in combination with yield were the criteria to assess the resistance depicted by them. The studies showed that among aromatic genotypes, 'Khushboo-95' gave the best yield of grain and harboured the least pest infestation (2.81% dead hearts and 1.85% white heads); on the other hand variety 'Sonahri Sugdasi (P)' harboured the highest borers attack (10.37% and 19.30%) and yielded the lowest grain yield. Regarding non-aromatic genotypes, IR8-2.5-11 received least infestation (1.32% and 0.26% dead hearts and white heads, respectively) generating highest yield showing its tolerance to borer's attack, in contrast, genotype IR6-252 harboured the highest infestation (5.65%, 4.28%) and yielded minimum grain indicating its susceptibility. These results demonstrate the expression of resistance gene in the genome of tolerant rice genotypes that can provide season-long protection from the natural infestation of insect pests. (author)

Studies on growth, yield and shoot and fruit borer (Leucinodes orbonalis Guenee.) resistance in M3 progenies of Solanum macrocarpon L

International Nuclear Information System (INIS)

Srinivasappa, K.N.; Ramanjini Gowda, P.H.; Thimmaiah, S.K.; Mahadevu, P.

1998-01-01

Shoot and fruit borer (Leucinodes orbonalis Guenee) is a serious pest in brinjal and no variety is found to be resistant to this dreaded pest. Solanum macrocarpon, a wild relative of brinjal is found to be resistant to shoot and fruit borer infestation. Attempts were made to hybridise between S. melongena and S. macrocarpon, but it was unsuccessful due to ovule abortion. The present study was undertaken to evaluate the promising mutants in M 3 generation for improved fruit quality besides its unbroken resistance and for further crop improvement

Estimation of genetic divergence in rice (oryza sativa l) germplasms on the basis of paddy yield and rice stem borer's (pyralidae: lepidoptera) resistance

International Nuclear Information System (INIS)

Sarwar, M.

2013-01-01

Field trials were carried out to estimate resistance along with paddy yield in 55 rice germplasm lines (35 aromatic and 20 non-aromatic genotypes) for rice stem borers (Pyralidae: Lepidoptera) to expose their potential in pest management approach. The results expressed significant differences for pest damage build-up and paddy yield among the rice germplasm lines. The findings clearly portrayed that based upon the percentage of pest invasions (dead hearts and white heads damage), no genotype was exclusively resistant to stem borers damage under field conditions. Two aromatic genotypes, Jajai-15A/97 and Basmati-Cr-34, exhibited least borers prevalence and amplified paddy yield while Sonehri Sugdasi (P) and Sada Gulab (P) pointed out a peak pest invasion and declined paddy yield. The estimation of pest incidence build-up and paddy productivity within non-aromatic genotypes confirmed that IR8 (P), IR6-15-2 and IR6 (P) were mainly proficient for bearing condensed pest invasion and augmented paddy yield. IR8-2.5-4, IR6-15-10 and IR6-20-9 demonstrated elevated pest susceptibility and gave poor yield. Rest of the germplasms appeared to be least tolerant or vulnerable to pest build-up and reduced paddy production. The tolerant and high yielding genotypes should be popularised in rice borers endemic areas and can be used in varietals resistance breeding strategy. The outcome of current studies necessitates the integration of existing host plant tolerance along with other management strategies to accomplish a suitable control of rice stem borers and enhance paddy yield. (author)

Heat treatment of Firewood for Emerald Ash Borer (Agrilus planipennis Fairmaire): Case Studies

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Xiping Wang; Richard D. Bergman; Brian K. Brashaw; Scott W. Myers

2014-01-01

The movement of firewood within emerald ash borer (Agrilus planipennis Fairmaire) (EAB)-infested states and into adjoining areas has been a contributor to its spread throughout the United States and Canada. In an effort to prevent further human-aided spread of EAB and to facilitate interstate commerce, the USDA Animal and Plant Health Inspection Service and cooperating...

INFLUENCE OF EUROPEAN CORN BORER (Ostrinia nubilalis Hübner ON CORN HYBRIDS IN NORTH-WEST AND EASTERN CROATIA

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Zvjezdana Augustinović

2005-12-01

Full Text Available European corn borer (Ostrinia nubilalis Hubner is one of the major corn pest in the world and in Croatia. Former investigations of corn borer in Croatia covered mostly its Eastern region. In trials conducted in 1998 and 1999 the research was extended to the North Western part of Croatia too. Macro trials were carried out with corn hybrids of FAO groups 200-600 at three localities: Križevci, Agricultural institute Osijek and at «Belje» PIK Karanac. In 1998 the intensity of the corn borer attack at the locality of «Belje» PIK Karanac was about 37.92% and in Agricultural institute Osijek 80.83%. In 1999 it varied between 37.08% at the locality of Agricultural Institute Osijek and 71.20% at the locality in Križevci. The estimated number of holes per plant in all three localities in both years was higher than the number of caterpillars. Length of damage per plant was between 0.38 and 18.80 cm. The data showed significant differences in the intensity of damaging effects on different localities while no significant differences concerning various hybrids were found. The statistical data concerning yield in both years showed significant differences among hybrids, localities and their interactions.

Ohmic heating of peaches in the wide range of frequencies (50 Hz to 1 MHz).

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Shynkaryk, Mykola V; Ji, Taehyun; Alvarez, Valente B; Sastry, Sudhir K

2010-09-01

The ohmic heating (OH) rate of peaches was studied at fixed electric field strength of 60 V.cm⁻¹, square-shaped instant reversal bipolar pulses, and frequencies varying within 50 Hz to 1 MHz. Thermal damage of tissue was evaluated from electrical admittivity. It showed that the time for half disruption (τ(T)) of tissue was required more than 10 h at temperatures below 40 °C. However, cellular thermal disruption occurred almost instantly (τ(T) 90 °C). Electrical conductivity σ(o) and admittivity σ(o)* of tissue at T(o)= 0 °C and their temperature coefficients (m, m*) were calculated. For freeze-thawed tissues, σ and σ* as well as m and m* were nearly indifferent to the frequency. However, for the intact tissue, both σ(o), σ(o)* and m, m* were frequency dependent. For freeze-thawed product, the power factor (P) was approximately equal to 1 and indifferent to the frequency and temperature. On the other hand, strong frequency dependence was observed for intact tissue with the minimum P approximately equal to 0.68 in the range of tens of kHz. The time required to reach a target temperature t(f) was evaluated. The t(f) increased with frequency up to the middle of the range of tens of kHz and thereafter continuously decreased. Samples exposed to the low-frequency electric field demonstrated faster electro-thermal damage rates. The textural relaxation data supported more intense damage kinetics at low-frequency OH. It has been demonstrated that a combination of high-frequency OH with pasteurization at moderate temperature followed by rapid cooling minimizes texture degradation of peach tissue. In this study, we investigated the electric field frequency effect on the rate of OH of peaches. It was shown that the time required for reaching the target temperature is strongly dependent upon the frequency. Samples exposed to low-frequency OH demonstrated higher electro-thermal damage rates. It has been shown that the combination of high-frequency OH with

Divergence of the bZIP Gene Family in Strawberry, Peach, and Apple Suggests Multiple Modes of Gene Evolution after Duplication

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Xiao-Long Wang

2015-01-01

Full Text Available The basic leucine zipper (bZIP transcription factors are the most diverse members of dimerizing transcription factors. In the present study, 50, 116, and 47 bZIP genes were identified in Malus domestica (apple, Prunus persica (peach, and Fragaria vesca (strawberry, respectively. Species-specific duplication was the main contributor to the large number of bZIPs observed in apple. After WGD in apple genome, orthologous bZIP genes corresponding to strawberry on duplicated regions in apple genome were retained. However, in peach ancestor, these syntenic regions were quickly lost or deleted. Maybe the positive selection contributed to the expansion of clade S to adapt to the development and environment stresses. In addition, purifying selection was mainly responsible for bZIP sequence-specific DNA binding. The analysis of orthologous pairs between chromosomes indicates that these orthologs derived from one gene duplication located on one of the nine ancient chromosomes in the Rosaceae. The comparative analysis of bZIP genes in three species provides information on the evolutionary fate of bZIP genes in apple and peach after they diverged from strawberry.

Effect of regulated deficit irrigation and crop load on the antioxidant compounds of peaches.

Science.gov (United States)

Buendía, Begoña; Allende, Ana; Nicolás, Emilio; Alarcón, Juan J; Gil, Maria I

2008-05-28

The use of regulated deficit irrigation (RDI) strategies is becoming a common practice in areas with low water availability. Little information is available about the effects of RDI on the antioxidant content of fruits. In this study, the influence of RDI on the content of vitamin C, phenolic compounds and carotenoids was investigated. Two irrigation strategies, fully irrigated (FI) and RDI, were compared at two levels of thinning, commercial and half of the commercial crop load. RDI strategies affected the content of vitamin C, phenolics and carotenoids of Flordastar peaches. RDI caused fruit peel stress lowering the content of vitamin C and carotenoids, while increasing the phenolic content, mainly anthocyanins and procyanidins. Fruit weight was the only quality index influenced by the crop load as it increased in FI fruits at low crop load. In general, fruits from commercial crop load had slightly higher content of antioxidants to fruits from low crop load, although these influences were only observed in the peel. Additionally, the influence of irrigation controlled by two sensors related to plant water level, maximum daily trunk shrinkage (MDS) and sap flow (SF) on the antioxidant constituents of peaches was evaluated. The response of the fruits to SF sensor was similar to that observed for RDI strategy. According to the tested water sensors, SF did not act as a good plant-based water indicator for use in irrigation scheduling, as it caused an increase in the content of phenolics, similar to that observed for fruits subjected to RDI. Therefore, selection of RDI strategies and plant water indicators should be taken into account as they affect the content of antioxidants of peaches.

Multiple R2R3-MYB transcription factors involved in the regulation of anthocyanin accumulation in peach flower

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Hui Zhou

2016-10-01

Full Text Available Anthocyanin accumulation is responsible for flower coloration in peach. Here, we report the identification and functional characterization of eight flavonoid-related R2R3-MYB transcription factors, designated PpMYB10.2, PpMYB9, PpMYBPA1, Peace, PpMYB17, PpMYB18, PpMYB19 and PpMYB20, respectively, in peach flower transcriptome. PpMYB10.2 and PpMYB9 are able to activate transcription of anthocyanin biosynthetic genes, whilst PpMYBPA1 and Peace have a strong activation on the promoters of proanthocyanin (PA biosynthetic genes. PpMYB17-20 show a strong repressive effect on transcription of flavonoid pathway genes such as DFR. These results indicate that anthocyanin accumulation in peach flower is coordinately regulated by a set of R2R3-MYB genes. In addition, PpMYB9 and PpMYB10.2 are closely related but separated into two groups, designated MYB9 and MYB10, respectively. PpMYB9 shows a strong activation on the PpUGT78A2 promoter, but with no effect on the promoter of PpUGT78B (commonly called PpUFGT in previous studies. In contrast, PpMYB10.2 is able to activate the PpUFGT promoter, but not for the PpUGT78A2 promoter. Unlike the MYB10 gene that is universally present in plants, the MYB9 gene is lost in most dicot species. Therefore, the PpMYB9 gene represents a novel group of anthocyanin-related MYB activators, which may have diverged in function from the MYB10 genes. Our study will aid in understanding the complex mechanism regulating floral pigmentation in peach and functional divergence of the R2R3-MYB gene family in plants.

Lipid-dissolved γ-carotene, β-carotene, and lycopene in globular chromoplasts of peach palm (Bactris gasipaes Kunth) fruits.

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Hempel, Judith; Amrehn, Evelyn; Quesada, Silvia; Esquivel, Patricia; Jiménez, Víctor M; Heller, Annerose; Carle, Reinhold; Schweiggert, Ralf M

2014-11-01

High levels of β-carotene, lycopene, and the rare γ-carotene occur predominantly lipid-dissolved in the chromoplasts of peach palm fruits. First proof of their absorption from these fruits is reported. The structural diversity, the physical deposition state in planta, and the human bioavailability of carotenoids from the edible fruits of diverse orange and yellow-colored peach palm (Bactris gasipaes Kunth) varieties were investigated. HPLC-PDA-MS(n) revealed a broad range of carotenes, reaching total carotenoid levels from 0.7 to 13.9 mg/100 g FW. Besides the predominant (all-E)-β-carotene (0.4-5.4 mg/100 g FW), two (Z)-isomers of γ-carotene (0.1-3.9 mg/100 g FW), and one (Z)-lycopene isomer (0.04-0.83 mg/100 g FW) prevailed. Approximately 89-94 % of total carotenoid content pertained to provitamin A carotenoids with retinol activity equivalents ranging from 37 to 609 µg/100 g FW. The physical deposition state of these carotenoids in planta was investigated using light, transmission electron, and scanning electron microscopy. The plastids found in both orange and yellow-colored fruit mesocarps were amylo-chromoplasts of the globular type, containing carotenoids predominantly in a lipid-dissolved form. The hypothesis of lipid-dissolved carotenoids was supported by simple solubility estimations based on carotenoid and lipid contents of the fruit mesocarp. In our study, we report first results on the human bioavailability of γ-carotene, β-carotene, and lycopene from peach palm fruit, particularly proving the post-prandial absorption of the rarely occurring γ-carotene. Since the physical state of carotenoid deposition has been shown to be decisive for carotenoid bioavailability, lipid-dissolved carotenoids in peach palm fruits are expected to be highly bioavailable, however, further studies are required.

Isolation and expression analysis of four HD-ZIP III family genes targeted by microRNA166 in peach.

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Zhang, C H; Zhang, B B; Ma, R J; Yu, M L; Guo, S L; Guo, L

2015-10-30

MicroRNA166 (miR166) is known to have highly conserved targets that encode proteins of the class III homeodomain-leucine zipper (HD-ZIP III) family, in a broad range of plant species. To further understand the relationship between HD-ZIP III genes and miR166, four HD-ZIP III family genes (PpHB14, PpHB15, PpHB8, and PpREV) were isolated from peach (Prunus persica) tissue and characterized. Spatio-temporal expression profiles of the genes were analyzed. Genes of the peach HD-ZIP III family were predicted to encode five conserved domains. Deduced amino acid sequences and tertiary structures of the four peach HD-ZIP III genes were highly conserved, with corresponding genes in Arabidopsis thaliana. The expression level of four targets displayed the opposite trend to that of miR166 throughout fruit development, with the exception of PpHB14 from 35 to 55 days after full bloom (DAFB). This finding indicates that miR166 may negatively regulate its four targets throughout fruit development. As for leaf and phloem, the same trend in expression level was observed between four targets and miR166 from 75 to 105 DAFB. However, the opposite trend was observed for the transcript level between four targets and miR166 from 35 to 55 DAFB. miRNA166 may negatively regulate four targets in some but not all developmental stages for a given tissue. The four genes studied were observed to have, exactly or generally, the same change tendency as individual tissue development, a finding that suggests genes of the HD-ZIP III family in peach may have complementary or cooperative functions in various tissues.

Environmental effects on fruit ripening and average fruit weight for three peach cultivars

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Three peach cultivars, ‘Crimson Lady’ (early), ‘Redhaven’ (mid-season) and ‘Cresthaven’ (late), were planted at twelve locations within the USA in 2009. All trees were grafted on ‘Lovell’ rootstock and came from the same nursery. Five trees of each cultivar were planted at a spacing of 6m by 5m at e...

Evaluation of firewood bagging and vacuum treatment for regulatory control of emerald ash borer

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Therese M. Poland; Tina M. Kuhn; Chen Zhangjing; Andrea Diss-Torrance; Erin L. Clark

2008-01-01

Since its discovery in Detroit, Michigan, in 2002, the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), has caused extensive mortality of ash (Fraxinus spp.) as it has spread across southeast Michigan, Ohio, and Ontario, Canada (Haack et al. 2002, Poland and McCullough 2006). In addition to this core...

Spinosad and the Tomato Borer Tuta absoluta: A Bioinsecticide, an Invasive Pest Threat, and High Insecticide Resistance

Science.gov (United States)

Campos, Mateus R.; Rodrigues, Agna Rita S.; Silva, Wellington M.; Silva, Tadeu Barbosa M.; Silva, Vitória Regina F.; Guedes, Raul Narciso C.; Siqueira, Herbert Alvaro A.

2014-01-01

The introduction of an agricultural pest species into a new environment is a potential threat to agroecosystems of the invaded area. The phytosanitary concern is even greater if the introduced pest’s phenotype expresses traits that will impair the management of that species. The invasive tomato borer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), is one such species and the characterization of the insecticide resistance prevailing in the area of origin is important to guide management efforts in new areas of introduction. The spinosad is one the main insecticides currently used in Brazil for control of the tomato borer; Brazil is the likely source of the introduction of the tomato borer into Europe. For this reason, spinosad resistance in Brazilian populations of this species was characterized. Spinosad resistance has been reported in Brazilian field populations of this pest species, and one resistant population that was used in this study was subjected to an additional seven generations of selection for spinosad resistance reaching levels over 180,000-fold. Inheritance studies indicated that spinosad resistance is monogenic, incompletely recessive and autosomal with high heritability (h 2 = 0.71). Spinosad resistance was unstable without selection pressure with a negative rate of change in the resistance level ( = −0.51) indicating an associated adaptive cost. Esterases and cytochrome P450-dependent monooxygenases titration decreased with spinosad selection, indicating that these detoxification enzymes are not the underlying resistance mechanism. Furthermore, the cross-resistance spectrum was restricted to the insecticide spinetoram, another spinosyn, suggesting that altered target site may be the mechanism involved. Therefore, the suspension of spinosyn use against the tomato borer would be a useful component in spinosad resistance management for this species. Spinosad use against this species in introduced areas should be carefully monitored to

Foliar nutrients explain goldspotted oak borer, Agrilus auroguttatus, adult feeding preference among four California oak species

Science.gov (United States)

Yigen Chen; Tom. W. Coleman; Michael. I. Jones; Mary. L. Flint; Steven. J. Seybold

2013-01-01

Adults of the invasive goldspotted oak borer, Agrilus auroguttatus Schaeffer (Coleoptera: Buprestidae), consumed foliar weight in no-choice feeding tests of, in descending order, California black oak Quercus kelloggii Newb., Engelmann oak, Quercus engelmannii Greene, coast live oak, Quercus...

Comparison of the measured specific activities of cesium in mushrooms, pine tree twigs, blueberries, honey and game in Aachen after 1986

International Nuclear Information System (INIS)

Bonka, H.; Schmelz, G.

1998-01-01

After the nuclear reactor accident at Chernobyl the specific activity in mushrooms originating from the region of Aachen was continuously measured until today. At the same time the specific activity was determined in pine tree twigs, blueberries, honey and game. There is a strong connection of the living organisms and the inanimate environment within the forest ecosystem. The decrease of the specific caesium activity in living organisms is slower than in the other environment. (orig.) [de

The crucial role of PpMYB10.1 in anthocyanin accumulation in peach and relationships between its allelic type and skin color phenotype.

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Tuan, Pham Anh; Bai, Songling; Yaegaki, Hideaki; Tamura, Takayuki; Hihara, Seisuke; Moriguchi, Takaya; Oda, Kenji

2015-11-18

Red coloration of fruit skin is one of the most important traits in peach (Prunus persica), and it is mainly due to the accumulation of anthocyanins. Three MYB10 genes, PpMYB10.1, PpMYB10.2, and PpMYB10.3, have been reported as important regulators of red coloration and anthocyanin biosynthesis in peach fruit. In this study, contribution of PpMYB10.1/2/3 to anthocyanin accumulation in the fruit skin was investigated in the Japanese peach cultivars, white-skinned 'Mochizuki' and red-skinned 'Akatsuki'. We then investigated the relationships between allelic type of PpMYB10.1 and skin color phenotype in 23 Japanese peach cultivars for future establishment of DNA-marker. During the fruit development of 'Mochizuki' and 'Akatsuki', anthocyanin accumulation was observed only in the skin of red 'Akatsuki' fruit in the late ripening stages concomitant with high mRNA levels of the last step gene leading to anthocyanin accumulation, UDP-glucose:flavonoid-3-O-glucosyltransferase (UFGT). This was also correlated with the expression level of PpMYB10.1. Unlike PpMYB10.1, expression levels of PpMYB10.2/3 were low in the skin of both 'Mochizuki' and 'Akatsuki' throughout fruit development. Moreover, only PpMYB10.1 revealed expression levels associated with total anthocyanin accumulation in the leaves and flowers of 'Mochizuki' and 'Akatsuki'. Introduction of PpMYB10.1 into tobacco increased the expression of tobacco UFGT, resulting in higher anthocyanin accumulation and deeper red transgenic tobacco flowers; however, overexpression of PpMYB10.2/3 did not alter anthocyanin content and color of transgenic tobacco flowers when compared with wild-type flowers. Dual-luciferase assay showed that the co-infiltration of PpMYB10.1 with PpbHLH3 significantly increased the activity of PpUFGT promoter. We also found close relationships of two PpMYB10.1 allelic types, MYB10.1-1/MYB10.1-2, with the intensity of red skin coloration. We showed that PpMYB10.1 is a major regulator of anthocyanin

Influence of microwave radiation on the post harvest decay and quality of peach fruits in cold storage

International Nuclear Information System (INIS)

Azarpajoun, E.; Nikkhah, SH.

2009-01-01

This research has been carried out in Khorasan Agricultural and Natural Resources research center to study the effect of microwave radiation on storage time and control of peach fruit rot. Peach cultivars (Alberta, Red, White and Green of Mashad) were harvested in the first and second decades of June, July and September, sorted and stored at 4°C for 12 hours. Then fruits were treated with a Microwave with the Frequency at 2450 MHZ and two intensities, low (200 w) and high (800 w) for 30, 60 and 120 seconds, the treated and control fruits were laid on in plastic trays, packed in perforated polyethylene bags and stored in cold storage (0°C and 90-95% relative humidity) for 2 months. The qualitative tests including fruit rot, total soluble solids, titrable acidity, weight loss, firmness and color were assayed after 15, 30, 45 and 60 days. Sensory attributes were measured after 60 days storage. The experimental design was factorial in frame of completely randomized design. Multiple range test (Duncan) were used to compare the means. The results showed that treating the fruit with microwave decreased the fruit rot and increased pH, flesh firmness and total soluble solids of treated peach. Microwave radiation with the 800w for 60 seconds maintained the qualitative characteristics of fruits. Panel test confirmed these results. (author)

Outreach and education efforts to counter the spread and impact of goldspotted oak borer

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Janis G. Gonzales; Thomas A. Scott; Kevin W. Turner; Lorin L. Lima

2015-01-01

The goldspotted oak borer (GSOB) Agrilus auroguttatus (Coleoptera: Buprestidae), has killed over 80,000 oaks across all land ownerships, costing over $8 million in public and private funds for mitigation and response. Linked to oak mortality in San Diego County in 2008, this exotic beetle likely arrived in California through infested firewood from...

The Role of Biocontrol of Emerald Ash Borer in Protecting Ash Regeneration after Invasion

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Emerald ash borer (EAB) is an invasive Asian beetle that is destroying ash in forests over much of eastern North America because of the high susceptibility of our native ash and a lack of effective natural enemies. To increase mortality of EAB larvae and eggs, the USDA (FS, ARS and APHIS) is carryin...

Influence of Inter stock Grafting as a Dwarfing Component on Peach Trees Development and Fruit Quality

International Nuclear Information System (INIS)

Hossain, S.; Nasrulhaq, A.; Mizutani, F.

2006-01-01

A field experiment was conducted to investigate the interstock grafting effect as a dwarfing component on Peach tree development and fruit quality. The study was made at the Ehime University Experimental farm located in south-eastern Japan during the period 2001-2005. The results of the field experiment indicated that pruned branches weight and flowers number were lower in interstock than in control trees. However, percent fruit set was a little higher in intersrock treated than in control trees. Fruit yield and fruit weight were lower in interstock treated than control trees. Soluble solids content and maturity index were higher in interstock treated trees than control, with an increasing trend in the period from 2001 to 2005, while titratable acidity showed that the livestock grafting is a useful dwarfing component for controlling the size of peach trees and improvement of fruit quality. (author)

Emerald Ash Borer Microbial Control with the Entomopathogen Beauveria bassiana GHA formulated as Botanigard®

Science.gov (United States)

Houping Lui; Leah S. Bauer

2008-01-01

The emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), a sporadic wood-boring pest native to northeastern Asia, was found attacking ash trees (Fraxinus spp.) in southeastern Michigan in 2002. Despite regulatory efforts to quarantine and eradicate EAB, this invasive beetle has continued to spread...

Interactive influence of leaf age, light intensity, and girdling on green ash foliar chemistry and emerald ash borer development.

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Chen, Yigen; Poland, Therese M

2009-07-01

Biotic and abiotic environmental factors affect plant nutritional quality and defensive compounds that confer plant resistance to herbivory. Influence of leaf age, light availability, and girdling on foliar nutrition and defense of green ash (Fraxinus pennsylvanica Marsh) was examined in this study. Longevity of the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), adults reared on green ash foliage subjected to these factors was assayed. Mature leaves generally were more nutritious with greater amino acids and a greater ratio of protein to non-structural carbohydrate (P:C) than young leaves, in particular when trees were grown in shade. On the other hand, mature leaves had lower amounts of trypsin and chymotrypsin inhibitors, and total phenolics compared to young leaves. Lower defense of mature leaves alone, or along with higher nutritional quality may lead to increased survival and longevity of emerald ash borer feeding on mature leaves. Sunlight reduced amino acids and P:C ratio, irrespective of leaf age and girdling, and elevated total protein of young foliage, but not protein of mature leaves. Sunlight also dramatically increased all investigated defensive compounds of young, but not mature leaves. Girdling reduced green ash foliar nutrition, especially, of young leaves grown in shade and of mature leaves grown in sun. However emerald ash borer performance did not differ when fed leaves from trees grown in sun or shade, or from girdled or control trees. One explanation is that emerald ash borer reared on lower nutritional quality food may compensate for nutrient deficiency by increasing its consumption rate. The strong interactions among leaf age, light intensity, and girdling on nutrition and defense highlight the need for caution when interpreting data without considering possible interactions.

Influence of Mortality Factors and Host Resistance on the Population Dynamics of Emerald Ash Borer (Coleoptera: Buprestidae) in Urban Forests.

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Macquarrie, Chris J K; Scharbach, Roger

2015-02-01

The success of emerald ash borer (Agrilus planipennis Fairmaire) in North America is hypothesized to be due to both the lack of significant natural enemies permitting easy establishment and a population of trees that lack the ability to defend themselves, which allows populations to grow unchecked. Since its discovery in 2002, a number of studies have examined mortality factors of the insect in forests, but none have examined the role of natural enemies and other mortality agents in the urban forest. This is significant because it is in the urban forest where the emerald ash borer has had the most significant economic impacts. We studied populations in urban forests in three municipalities in Ontario, Canada, between 2010 and 2012 using life tables and stage-specific survivorship to analyze data from a split-rearing manipulative experiment. We found that there was little overall mortality caused by natural enemies; most mortality we did observe was caused by disease. Stage-specific survivorship was lowest in small and large larvae, supporting previous observations of high mortality in these two stages. We also used our data to test the hypothesis that mortality and density in emerald ash borer are linked. Our results support the prediction of a negative relationship between mortality and density. However, the relationship varies between insects developing in the crown and those in the trunk of the tree. This relationship was significant because when incorporated with previous findings, it suggests a mechanism and hypothesis to explain the outbreak dynamics of the emerald ash borer. © The Author 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

The relationship between the emerald ash borer (Agrilus planipennis) and ash (Fraxinus spp.) tree decline: Using visual canopy condition assessments and leaf isotope measurements to assess pest damage

Science.gov (United States)

Charles E. Flower; Kathleen S. Knight; Joanne Rebbeck; Miquel A. Gonzalez-Meler

2013-01-01

Ash trees (Fraxinus spp.) in North America are being severely impacted by the invasive emerald ash borer (Agrilus planipennis Fairmaire) which was inadvertently introduced to the US in the 1990s from Asia. The emerald ash borer (EAB) is a phloem boring beetle which relies exclusively on ash trees to complete its life cycle. Larvae...

Ecoinformatics Can Infer Causal Effects of Crop Variety on Insect Attack by Capitalizing on 'Pseudoexperiments' Created When Different Crop Varieties Are Interspersed: A Case Study in Almonds.

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Rosenheim, Jay A; Higbee, Bradley S; Ackerman, Jonathan D; Meisner, Matthew H

2017-12-05

Capturing the complementary strengths of observational and experimental research methods usually requires the researcher to gather separate experimental and observational data sets. In some cases, however, commercial agricultural practices produce the spatial and temporal mixing of 'treatments' independently of other possibly covarying factors that is normally achieved only with formal experimentation. The resulting 'pseudoexperiments' can provide strong evidence for causal relationships. Here, we analyze a large observational data set that creates a series of such pseudoexperiments to assess the effect of different commercial varieties of almond, Prunus dulcis (Mill.) on the impact of two key lepidopteran pests, the navel orangeworm Amyelois transitella (Walker) (Lepidoptera: Pyralidae), and the peach twig borer Anarsia lineatella Zeller (Lepidoptera: Gelechiidae). Almonds are universally planted as polycultures of different varieties to obtain efficient cross-pollination. We find substantial differences across almond varieties in the rates of infestation of almond hulls and nutmeats by the two pests. We find no support for the hypothesis that earlier-maturing varieties sustain higher attack; for A. transitella, later-maturing varieties instead had more frequent infestation. On many almond varieties, A. lineatella reaches high infestation levels by feeding almost exclusively on the hulls, rather than nutmeats. Given the importance of these pests in directly destroying almond nuts and in promoting aflatoxin-producing Aspergillus sp. fungal infections of almonds, further work exploring the impact of these pests is warranted. Because many crops requiring cross-pollination are planted as mixtures of different varieties, commercial agricultural production data hold great potential for studying within-crop variation in susceptibility to insect attack. © The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights

Efficacy of multifunnel traps for capturing emerald ash borer (Coleoptera: Buprestidae): effect of color, glue, and other trap coatings.

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Francese, Joseph A; Fraser, Ivich; Lance, David R; Mastro, Victor C

2011-06-01

Tens of thousands of adhesive-coated purple prism traps are deployed annually in the United States to survey for the invasive emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae). A reusable, more user-friendly trap is desired by program managers, surveyors, and researchers. Field assays were conducted in southeastern Michigan to ascertain the feasibility of using nonsticky traps as survey and detection tools for emerald ash borer. Three nonsticky trap designs, including multifunnel (Lindgren), modified intercept panel, and drainpipe (all painted purple) were compared with the standard purple prism trap; no statistical differences in capture of emerald ash borer adults were detected between the multifunnel design and the prism. In subsequent color comparison assays, both green- and purple-painted multifunnel traps (and later, plastic versions of these colors) performed as well or better than the prism traps. Multifunnel traps coated with spray-on adhesive caught more beetles than untreated traps. The increased catch, however, occurred in the traps' collection cups and not on the trap surface. In a separate assay, there was no significant difference detected between glue-coated traps and Rain-X (normally a glass treatment)-coated traps, but both caught significantly more A. planipennis adults than untreated traps.

Patterns among the ashes: Exploring the relationship between landscape pattern and the emerald ash borer

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Susan J. Crocker; Dacia M. Meneguzzo; Greg C. Liknes

2010-01-01

Landscape metrics, including host abundance and population density, were calculated using forest inventory and land cover data to assess the relationship between landscape pattern and the presence or absence of the emerald ash borer (EAB) (Agrilus planipennis Fairmaire). The Random Forests classification algorithm in the R statistical environment was...

Effect of Nano Packaging on Storage Life and Quality Attributes of Elberta Peach

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Mohammadreza Asghari

2017-12-01

Full Text Available Introduction: Asaclimactericfruit, peach has a highrespiration rateandvery lowshelf life. Nowadays theuse ofappropriatepostharvesttechnologiesto increase fruit postharvest lifeis necessary.Use of nanotechnologyis considered asan effective method to increase fruit postharvest life. Nanotechnology isused extensivelyinallstages of production, processing, storage, packagingand transport ofagriculturalproducts.The objective of this study was to investigate the effect of silver and silica nanocomposites, nanosilver and polypropylene containers on shelf-life and preservation of peach qualitative characteristics (Prunuspersicacv. Elberta such as titrable acidity, soluble solids, ascorbic acid, total antioxidant and total phenolics content in cold storage after the harvest. Materials and Methods: To determine the effect of nanopackaging onshelf life andqualitativecharacteristics ofElberta peaches,the experiment was conducted in a factorial based on a completely randomized design with two treatmentsand four replications.The first factor consisted of nanopackagingnanosilver, silver and silica nanocompositesandpolypropylenepackaging, and the second factor was coldstorage periods of15, 30 and 45 daysattemperaturesfrom 0 to0/5 °C and a relative humidityof 85to 95%.In general, the experiment consisted of 36 experimental units, and each includinga container with four Elberta peach fruits.Data were analyzed by using SAS software, and Microsoft Office Excel 2010 software was used to plot the graphs.Duncan's multiple range test was used to compare the means of treatments. Results and Discussion: At the end of the maintenance period, the highest firmness of peach texture was related to nanosilver containers. The main cause of fruit softening is the destruction of cell wall components, especially pectin, which is caused by certain enzymes such as polygalactronase. Whenstorage timeincreased, polypropylene containers showed a higher weight loss compared to

Crafting traps with attractant alcoholics an alternative for monitoring and control of borer coffee, Hypothenemus hampei (Ferrari 1867

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Agramont Richard

2010-10-01

Full Text Available The necessity to incorporate an alternative, for monitoring and control of the borer coffee, Hypothenemus hampei (Ferrari (Choleoptera: Curculionidae to be feasible for the use of the coffee producers, in the community Choro, Coripata municipality, second section of Nor Yungas province, La Paz Bolivia. It was evaluated the capture of adult borer coffee individuals using 45 traps into 1,5 hectares distributed at random with four repetitions. It was used three types of craft traps, built with disposable plastic bottles of soft drinks, with the traps Casera, Brocap and Yessica, were evaluated three treatments: mixture of alcohols methyl (M and ethylic (E in proportions 3:1; mix 1:1 of (M and (E; mix 1:1:1 of (M (E and coffee fresh cherry liquated (CFCL and water as a witness. The largest captures of adult individuals, were present in the crafting traps with mixture of (M(E 3:1 with overalls (± standard deviation adults/traps/ten days of 3414,5±3227,7 being superior to the other treatments. The crafting trap is one of the alternatives for the control and monitoring of the borer in the coffee plantations. The use of crafting traps with alcoholic attractants for the capture of adult individuals, is present as a low cost alternative, being feasible the successful use by the producers into the management integrated programs.

Economic injury level for the coffee berry borer (Coleoptera: Curculionidae: Scolytinae) using attractive traps in Brazilian coffee fields.

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Fernandes, F L; Picanço, M C; Campos, S O; Bastos, C S; Chediak, M; Guedes, R N C; Silva, R S

2011-12-01

The currently existing sample procedures available for decision-making regarding the control of the coffee berry borer Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae) are time-consuming, expensive, and difficult to perform, compromising their adoption. In addition, the damage functions incorporated in such decision levels only consider the quantitative losses, while dismissing the qualitative losses. Traps containing ethanol, methanol, and benzaldehyde may allow cheap and easy decision-making. Our objective was to determine the economic injury level (EIL) for the adults of the coffee berry borer by using attractant-baited traps. We considered both qualitative and quantitative losses caused by the coffee borer in estimating the EILs. These EILs were determined for conventional and organic coffee under high and average plant yield. When the quantitative losses caused by H. hampei were considered alone, the EILs ranged from 7.9 to 23.7% of bored berries for high and average-yield conventional crops, respectively. For high and average-yield organic coffee the ELs varied from 24.4 to 47.6% of bored berries, respectively. When qualitative and quantitative losses caused by the pest were considered together, the EIL was 4.3% of bored berries for both conventional and organic coffee. The EILs for H. hampei associated to the coffee plants in the flowering, pinhead fruit, and ripening fruit stages were 426, 85, and 28 adults per attractive trap, respectively.

Root and aerial growth in early-maturing peach trees under two crop load treatments

Energy Technology Data Exchange (ETDEWEB)

Abrisqueta, I.; Conejero, W.; López-Martínez, L.; Vera, J.; Ruiz Sánchez, M.C.

2017-07-01

The objectives of the paper were to study the pattern of root growth (measured by minirhizotrons) in relation to trunk, fruit and shoot growth and the effects of crop load on tree growth and yield in peach trees. Two crop load (commercial and low) treatments were applied in a mature early-maturing peach tree orchard growing in Mediterranean conditions. Root growth dynamics were measured using minirhizotrons during one growing season. Shoot, trunk and fruit growth were also measured. At harvest, all fruits were weighed, counted and sized. Roots grew throughout the year but at lower rates during the active fruit growth phase. Root growth was asynchronous with shoot growth, while root and trunk growth rates were highest after harvest, when the canopy was big enough to allocate the photo-assimilates to organs that would ensure the following season’s yield. Shoot and fruit growth was greater in the low crop load treatment and was accompanied by a non-significant increase in root growth. High level of fruit thinning decreased the current yield but the fruits were more marketable because of their greater size.

Influence of trap color and host volatiles on capture of the emerald ash borer (Coleoptera: Buprestidae).

Science.gov (United States)

Crook, Damon J; Khrimian, Ashot; Cossé, Allard; Fraser, Ivich; Mastro, Victor C

2012-04-01

Field trapping assays were conducted in 2009 and 2010 throughout western Michigan, to evaluate lures for adult emerald ash borer, A. planipennis Fairmaire (Coleoptera: Buprestidae). Several ash tree volatiles were tested on purple prism traps in 2009, and a dark green prism trap in 2010. In 2009, six bark oil distillate lure treatments were tested against manuka oil lures (used in 2008 by USDA APHIS PPQ emerald ash borer cooperative program). Purple traps baited with 80/20 (manuka/phoebe oil) significantly increased beetle catch compared with traps baited with manuka oil alone. In 2010 we monitored emerald ash borer attraction to dark green traps baited with six lure combinations of 80/20 (manuka/phoebe), manuka oil, and (3Z)-hexenol. Traps baited with manuka oil and (3Z)-hexenol caught significantly more male and total count insects than traps baited with manuka oil alone. Traps baited with manuka oil and (3Z)-hexenol did not catch more beetles when compared with traps baited with (3Z)-hexenol alone. When compared with unbaited green traps our results show that (3Z)-hexenol improved male catch significantly in only one of three field experiments using dark green traps. Dark green traps caught a high number of A. planipennis when unbaited while (3Z)-hexenol was seen to have a minimal (nonsignificant) trap catch effect at several different release rates. We hypothesize that the previously reported kairomonal attractancy of (3Z)-hexenol (for males) on light green traps is not as obvious here because of improved male attractancy to the darker green trap.

Spatial distribution of grape root borer (Lepidoptera: Sesiidae) infestations in Virginia vineyards and implications for sampling.

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Rijal, J P; Brewster, C C; Bergh, J C

2014-06-01

Grape root borer, Vitacea polistiformis (Harris) (Lepidoptera: Sesiidae) is a potentially destructive pest of grape vines, Vitis spp. in the eastern United States. After feeding on grape roots for ≍2 yr in Virginia, larvae pupate beneath the soil surface around the vine base. Adults emerge during July and August, leaving empty pupal exuviae on or protruding from the soil. Weekly collections of pupal exuviae from an ≍1-m-diameter weed-free zone around the base of a grid of sample vines in Virginia vineyards were conducted in July and August, 2008-2012, and their distribution was characterized using both nonspatial (dispersion) and spatial techniques. Taylor's power law showed a significant aggregation of pupal exuviae, based on data from 19 vineyard blocks. Combined use of geostatistical and Spatial Analysis by Distance IndicEs methods indicated evidence of an aggregated pupal exuviae distribution pattern in seven of the nine blocks used for those analyses. Grape root borer pupal exuviae exhibited spatial dependency within a mean distance of 8.8 m, based on the range values of best-fitted variograms. Interpolated and clustering index-based infestation distribution maps were developed to show the spatial pattern of the insect within the vineyard blocks. The temporal distribution of pupal exuviae showed that the majority of moths emerged during the 3-wk period spanning the third week of July and the first week of August. The spatial distribution of grape root borer pupal exuviae was used in combination with temporal moth emergence patterns to develop a quantitative and efficient sampling scheme to assess infestations.

Temporal dynamics of woodpecker predation on the invasive emerald ash borer (Agrilus planipennis) in North America

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Woodpeckers (Picidae) are among the most prevalent natural enemies attacking the invasive emerald ash borer (EAB), Agrilus planipennis Fairmaire, in North America, but there can be considerable variation in the levels of EAB predation on ash trees (Oleaceae: Fraxinus) within and between sites as wel...

Serine proteases SP1 and SP13 mediate the melanization response of Asian corn borer, Ostrinia furnacalis, against entomopathogenic fungus Beauveria bassiana.

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Chu, Yuan; Liu, Yang; Shen, Dongxu; Hong, Fang; Wang, Guirong; An, Chunju

2015-06-01

Exposure to entomopathogenic fungi is one approach for insect pest control. Little is known about the immune interactions between fungus and its insect host. Melanization is a prominent immune response in insects in defending against pathogens such as bacteria and fungi. Clip domain serine proteases in insect plasma have been implicated in the activation of prophenoloxidase, a key enzyme in the melanization. The relationship between host melanization and the infection by a fungus needs to be established. We report here that the injection of entomopathogenic fungus Beauveria bassiana induced both melanin synthesis and phenoloxidase activity in its host insect, the Asian corn borer, Ostrinia furnacalis (Guenée). qRT-PCR analysis showed several distinct patterns of expression of 13 clip-domain serine proteases in response to the challenge of fungi, with seven increased, two decreased, and four unchanged. Of special interest among these clip-domain serine protease genes are SP1 and SP13, the orthologs of Manduca sexta HP6 and PAP1 which are involved in the prophenoloxidase activation pathway. Recombinant O. furnacalis SP1 was found to activate proSP13 and induce the phenoloxidase activity in corn borer plasma. Additionally, SP13 was determined to directly cleave prophenoloxidase and therefore act as the prophenoloxidase activating protease. Our work thus reveals a biochemical mechanism in the melanization in corn borer associated with the challenge by B. bassiana injection. These insights could provide valuable information for better understanding the immune responses of Asian corn borer against B. bassiana. Copyright © 2015 Elsevier Inc. All rights reserved.

Produção integrada e convencional de pêssegos cv. Marli Integrated and convetional production of peach cv. Marli

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José Luis da Silva Nunes

2004-12-01

Full Text Available O cultivo de pessegueiros é uma atividade de grande importância econômica no Sul do Brasil, onde se destaca o Estado do Rio Grande do Sul como maior produtor brasileiro. Um dos aspectos mais importantes na produção de alimentos da atualidade é a redução no uso de agroquímicos, com menor contaminação do ambiente e riscos reduzidos de resíduos. Este trabalho visou a comparar os sistemas de Produção Convencional (PC e Integrada (PI de pêssegos e foi realizado no ano de 2001, no município de São Jerônimo - RS, latitude 30°05'52" S, longitude 51°39'08" W e altitude de 46 metros. Áreas de um pomar comercial da cv. Marli foram avaliadas em relação às principais práticas de manejo da planta e do solo, controle fitossanitário, aspectos econômicos, bem como à qualidade da fruta. Na área conduzida sob PI, foram utilizadas as práticas de manejo preconizadas pelas Normas de Produção Integrada de Pêssegos (NPIP e, na área conduzida no sistema de PC, as plantas foram manejadas de acordo com as práticas comumente utilizadas pelo produtor. A produção de pêssegos, em ambos os sistemas, não foi afetada. Na área de PI, houve menor número de pêssegos por planta; entretanto, as frutas apresentaram maior peso médio. A maioria dos pêssegos da PI foram classificados como CAT I (diâmetro superior a 57 mm. As frutas produzidas na PC são, na maioria, de CAT II (de 48 a 57 mm. A qualidade pós-colheita não apresentou diferenças em relação à acidez, firmeza e cor. Com base nestes resultados, podemos concluir que é possível produzir pêssegos de qualidade com produtividade no sistema de PI.The peach-trees cultivation is a very economic important activity in South of Brazil, where highlights the State of Rio Grande do Sul as the largest Brazilian producer. One of the most important aspects in actuality is the food prodution with reduction of agrochemicals components, reducing the risks of environment contamination. This work

Cloning and functional analysis of 9-cis-epoxycarotenoid dioxygenase (NCED) genes encoding a key enzyme during abscisic acid biosynthesis from peach and grape fruits.

Science.gov (United States)

Zhang, Mei; Leng, Ping; Zhang, Guanglian; Li, Xiangxin

2009-08-15

Ripening and senescence are generally controlled by ethylene in climacteric fruits like peaches, and the ripening process of grape, a non-climacteric fruit, may have some relationship to abscisic acid (ABA) function. In order to better understand the role of ABA in ripening and senescence of these two types of fruits, we cloned the 9-cis-epoxycarotenoid dioxygenase (NCED) gene that encodes a key enzyme in ABA biosynthesis from peaches and grapes using an RT-PCR approach. The NCED gene fragments were cloned from peaches (PpNCED1and PpNCED2, each 740bp) and grapes (VVNCED1, 741bp) using degenerate primers designed based on the conserved amino acids sequence of NCEDs in other plants. PpNCED1 showed 78.54% homology with PpNCED2, 74.90% homology with VVNCED1, and both showed high homology to NCEDs from other plants. The expression patterns of PpNCED1 and VVNCED1 were very similar. Both were highly expressed at the beginning of ripening when ABA content becomes high. The maximum ABA preceded ethylene production in peach fruit. ABA in the grape gradually increased from the beginning of ripening and reached the highest level at 20d before the harvest stage. However, ethylene remained at low levels during the entire process of fruit development, including ripening and senescence. ABA content, and ripening and softening of both types of fruits, were promoted or delayed by exogenous ABA or Fluridone (or NDGA) treatment. The roles of ABA and ethylene in the later ripening of fruit are complex. Based on results obtained in this study, we concluded that PpNCED1 and VVNCED1 initiate ABA biosynthesis at the beginning of fruit ripening, and that ABA accumulation might play a key role in the regulation of ripeness and senescence of both peach and grape fruits.

Study of 'Redhaven' peach and its white-fleshed mutant suggests a key role of CCD4 carotenoid dioxygenase in carotenoid and norisoprenoid volatile metabolism

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Tartarini Stefano

2011-01-01

Full Text Available Abstract Background Carotenoids are plant metabolites which are not only essential in photosynthesis but also important quality factors in determining the pigmentation and aroma of flowers and fruits. To investigate the regulation of carotenoid metabolism, as related to norisoprenoids and other volatile compounds in peach (Prunus persica L. Batsch., and the role of carotenoid dioxygenases in determining differences in flesh color phenotype and volatile composition, the expression patterns of relevant carotenoid genes and metabolites were studied during fruit development along with volatile compound content. Two contrasted cultivars, the yellow-fleshed 'Redhaven' (RH and its white-fleshed mutant 'Redhaven Bianca' (RHB were examined. Results The two genotypes displayed marked differences in the accumulation of carotenoid pigments in mesocarp tissues. Lower carotenoid levels and higher levels of norisoprenoid volatiles were observed in RHB, which might be explained by differential activity of carotenoid cleavage dioxygenase (CCD enzymes. In fact, the ccd4 transcript levels were dramatically higher at late ripening stages in RHB with respect to RH. The two genotypes also showed differences in the expression patterns of several carotenoid and isoprenoid transcripts, compatible with a feed-back regulation of these transcripts. Abamine SG - an inhibitor of CCD enzymes - decreased the levels of both isoprenoid and non-isoprenoid volatiles in RHB fruits, indicating a complex regulation of volatile production. Conclusions Differential expression of ccd4 is likely to be the major determinant in the accumulation of carotenoids and carotenoid-derived volatiles in peach fruit flesh. More in general, dioxygenases appear to be key factors controlling volatile composition in peach fruit, since abamine SG-treated 'Redhaven Bianca' fruits had strongly reduced levels of norisoprenoids and other volatile classes. Comparative functional studies of peach carotenoid

Physicochemical characterization of the peach palm (Bactris gasipaes Kunth, Arecaceae fruit skin residue flour obtained by convective drying

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Jader Martínez-Girón

2017-09-01

Full Text Available The production of flour from residues of peach -palm fruit skin has been a forgotten topic from the view point of its physicochemical properties, because studies reported in the literature have focused on mesocarp flour characterization. The objective of this study was to evaluate the physicochemical properties of the peach palm fruit epicarp flour obtained by convective drying at 60±2 °C through a specific production and storage process. As a result, the flour was found to be a source of bioactive compounds, showing total phenol concentration (23.40±1.30 mg gallic acid/100 g, antioxidant activity (33.10%±3.20, total carotenoids (59.31±1.61 mg β-carotene/100 g, color a* (4.95±0.58, color b* (3.25±0.57, and luminosity (33.95±3.16. On the other hand, there was a high level of total carotenoids (85% and phenolic compounds (94% retention after six months. Additionally, color change showed no significant difference during storage. In conclusion, the flour obtained is a byproduct with properties that can be used as an alternative to agroalimentary substitutes, mainly due to its color and antioxidant activity attributes. It is noteworthy to mention that this is one of the first studies to physicochemically characterize peach palm fruit skin flour from Valle del Cauca (Colombia.

Conservation implications of the mating system of the Pampa Hermosa landrace of peach palm analyzed with microsatellite markers.

Science.gov (United States)

Picanço-Rodrigues, Doriane; Astolfi-Filho, Spartaco; Lemes, Maristerra R; Gribel, Rogerio; Sebbenn, Alexandre M; Clement, Charles R

2015-03-01

Peach palm (Bactris gasipaes) is cultivated by many indigenous and traditional communities from Amazonia to Central America for its edible fruits, and is currently important for its heart-of-palm. The objective of this study was to investigate the mating system of peach palm, as this is important for conservation and breeding. Eight microsatellite loci were used to genotype 24 open-pollinated progenies from three populations of the Pampa Hermosa landrace maintained in a progeny trial for genetic improvement. Both the multi-locus outcrossing rates (0.95 to 0.99) and the progeny level multi-locus outcrossing rates (0.9 to 1.0) were high, indicating that peach palm is predominantly allogamous. The outcrossing rates among relatives were significantly different from zero (0.101 to 0.202), providing evidence for considerable biparental inbreeding within populations, probably due to farmers planting seeds of a small number of open-pollinated progenies in the same plot. The correlations of paternity estimates were low (0.051 to 0.112), suggesting a large number of pollen sources (9 to 20) participating in pollination of individual fruit bunches. Effective population size estimates suggest that current germplasm collections are insufficient for long-term ex situ conservation. As with most underutilized crops, on farm conservation is the most important component of an integrated conservation strategy.

Conservation implications of the mating system of the Pampa Hermosa landrace of peach palm analyzed with microsatellite markers

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Doriane Picanço-Rodrigues

2015-03-01

Full Text Available Peach palm (Bactris gasipaes is cultivated by many indigenous and traditional communities from Amazonia to Central America for its edible fruits, and is currently important for its heart-of-palm. The objective of this study was to investigate the mating system of peach palm, as this is important for conservation and breeding. Eight microsatellite loci were used to genotype 24 open-pollinated progenies from three populations of the Pampa Hermosa landrace maintained in a progeny trial for genetic improvement. Both the multi-locus outcrossing rates (0.95 to 0.99 and the progeny level multi-locus outcrossing rates (0.9 to 1.0 were high, indicating that peach palm is predominantly allogamous. The outcrossing rates among relatives were significantly different from zero (0.101 to 0.202, providing evidence for considerable biparental inbreeding within populations, probably due to farmers planting seeds of a small number of open-pollinated progenies in the same plot. The correlations of paternity estimates were low (0.051 to 0.112, suggesting a large number of pollen sources (9 to 20 participating in pollination of individual fruit bunches. Effective population size estimates suggest that current germplasm collections are insufficient for long-term ex situ conservation. As with most underutilized crops, on farm conservation is the most important component of an integrated conservation strategy.

SELECTIVITY OF INSECTICIDES USED IN PEACH FARMING TO LARVAE OF Chrysoperla externa (NEUROPTERA: CHRYSOPIDAE IN SEMI-FIELD CONDITIONS

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RODOLFO VARGAS CASTILHOS

2017-01-01

Full Text Available The selectivity of five insecticides, regularly used in peach farming, was assessed for larvae of the predator Chrysoperla externa (Hagen (Neuroptera: Chrysopidae by means of bioassay in semi-field conditions. The bioassay was based on the counting of captured larvae after release in peach trees treated with the insecticides (% of active ingredient in spray liquid: deltamethrin (0.001, fenthion (0.050, phosmet (0.100, lufenuron (0.005 and malathion (0.200. Bait-cards with eggs of Anagasta kuehniella (Zeller (Lepidoptera: Pyralidae were used to capture larvae from treated plants; five of them were fixed in plant canopy and five others left on the soil around stem. A protective barrier made up of galvanized steel sheet was used for each plant to avoid loss of larvae. The number of larvae feeding on the bait-cards was measured for four days. According to the number of captured larvae, each insecticide effect was estimated and classified into toxicity categories as stated by the International Organization for Biological and Integratec Control of Noxious Animals and Plants (IOBC. Based on observations, the insect growth regulator lufenuron was harmless, while the neurotoxins deltamethrin and malathion were slightly harmful; and lastly, fenthion and phosmet were moderately harmful to C. externa larvae in semi-field conditions. Thus, lufenuron should be recommended for integrated pest management, since it would preserve this predator species in peach orchards.

Studies on the possibilities of using a by-product resulting during the extraction of the volatile oils from conifer twigs and needles in the leather industry

Energy Technology Data Exchange (ETDEWEB)

Chirita, A.; Toma, A.R.; Cocis, V.

1979-01-01

Organic by-products containing 19-40% tannin from the water extraction of volatile oils from spruce bark, twigs, and needles were useful when mixed in a 1:1 ratio with BCF synthetic tannin auxiliary to tan hide, and similar by-products containing 16-31% reducing substances could be used to replace glucose as reducing agent in chrome tanning.

Determining The Factors Affecting Fruit Hardness of Different Peach Types with Meta Analysis

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Hande Küçükönder

2014-09-01

Full Text Available The aim of this study is to determine the factor effective in determining the hardness of Caterina, Suidring, Royal Glory and Tirrenia peach types using meta analysis. In the study, the impact force (Fi and the contact time (tc were detected and the impulse values (I that are expressed as independent variable in the area under the curve were calculated in the measurements performed using the technique of a low-mass lateral impactor multiplicated with peach. Using the theory of elasticity, the independent variables were determined as Fmax (maximum impact force, contact time (tmax, Fmax/tmax, 1/tmax, 1/tmax2,5, Fmax/tmax 1.25 and Fmax2.5 parameters. The correlation coefficient values showing the relationship between these parameters and the dependent variable Magness-Taylor force (MT were calculated and were combined with meta-analysis by using the Hunter-Schmid and Fisher’s Z methods. The Cohen’s classification criterion was used in evaluating the resulting mean effect size (combined correlation value and in determining its direction. As a result of the meta-analysis, the mean effect size according to Hunter-Schmid method was found 0.436 (0.371-0.497 positively directed in 95% confidence interval, while it was found 0.468 (0.390-0.545 according to Fisher’s Z method. The effect sizes in both methods were determined “mid-level” according to the Cohen’s classification. When the significance level of the studies was analyzed with the Z test, all of the ones that taken into the meta analysis has been found statistically significant. As a result of the meta analysis in this study evaluating the relationship of peach types with the fruit hardness, the mean effect size has been found to reach “strong level”. Consequently, “maximum shock acceleration” was found to be a more effective factor comparing to the other factors in determining the the fruit hardness according to the results of meta analysis applied in both methods.

Summary of Preliminary Criticality Analysis for Peach Bottom Fuel in the DOE Standardized Spent Nuclear Fuel Canister

International Nuclear Information System (INIS)

Henrikson, D.J.

1999-01-01

The Department of Energy's (DOE's) National Spent Nuclear Fuel Program is developing a standardized set of canisters for DOE spent nuclear fuel (SNF). These canisters will be used for DOE SNF handling, interim storage, transportation, and disposal in the national repository. Several fuels are being examined in conjunction with the DOE SNF canisters. This report summarizes the preliminary criticality safety analysis that addresses general fissile loading limits for Peach Bottom graphite fuel in the DOE SNF canister. The canister is considered both alone and inside the 5-HLW/DOE Long Spent Fuel Co-disposal Waste Package, and in intact and degraded conditions. Results are appropriate for a single DOE SNF canister. Specific facilities, equipment, canister internal structures, and scenarios for handling, storage, and transportation have not yet been defined and are not evaluated in this analysis. The analysis assumes that the DOE SNF canister is designed so that it maintains reasonable geometric integrity. Parameters important to the results are the canister outer diameter, inner diameter, and wall thickness. These parameters are assumed to have nominal dimensions of 45.7-cm (18.0-in.), 43.815-cm (17.25-in), and 0.953-cm (0.375-in.), respectively. Based on the analysis results, the recommended fissile loading for the DOE SNF canister is 13 Peach Bottom fuel elements if no internal steel is present, and 15 Peach Bottom fuel elements if credit is taken for internal steel

Development of a web-based tool for projecting costs of managing emerald ash borer in municipal forests

Science.gov (United States)

Clifford S. Sadof

2009-01-01

City managers faced with the invasion of emerald ash borer into their urban forests need to plan for the invasion in order to obtain the resources they need to protect the public from harm caused by dying ash trees. Currently, city...

Monitoring ash (Fraxinus spp.) decline and emerald ash borer (Agrilus planipennis) symptoms in infested areas

Science.gov (United States)

Kathleen S. Knight; Britton P. Flash; Rachel H. Kappler; Joel A. Throckmorton; Bernadette Grafton; Charles E. Flower

2014-01-01

Emerald ash borer (A. planipennis) (EAB) has had a devastating effect on ash (Fraxinus) species since its introduction to North America and has resulted in altered ecological processes across the area of infestation. Monitoring is an important tool for understanding and managing the impact of this threat, and the use of common...

The Hawaii protocol for scientific monitoring of coffee berry borer: a model for coffee agroecosystems worldwide

Science.gov (United States)

Coffee Berry Borer (CBB) is the most devastating insect pest for coffee crops worldwide. We developed a scientific monitoring protocol aimed at capturing and quantifying the dynamics and impact of this invasive insect pest as well as the development of its host plant across a heterogeneous landscape...

Attraction of the emerald ash borer to ash trees stressed by girdling, herbicide treatment, or wounding

Science.gov (United States)

Deborah McCullough; Therese Poland; David. Cappaert

2009-01-01

New infestations of emerald ash borer, Agrilus planipennis Fairmaire, an invasive pest native to Asia, are difficult to detect until densities build and symptoms appear on affected ash (Fraxinus spp). We compared the attraction of A. planipennis to ash trees stressed by girdling (bark and phloem removed...

Disinfestation of litchi stem-end borer Conopomorpha sinensis Bradley with irradiation for export of litchi fruits

International Nuclear Information System (INIS)

Hu Meiying; Liu Xiuqiong; Hou Renhuan; Li Xiaodong; Yao Zhenwei; Lou Xuemei; Weng Qunfang

1999-01-01

Larvae of the litchi stem-end borer, Conopomorpha sinensis Bradley, in litchi fruits were exposed to 60 Co gamma irradiation doses ranging from 0-400 Gy as a quarantine treatment. Criterion of effectiveness of the irradiation dose was based on preventing adults emerging from treated fruits infested with the larvae. Probit analysis showed that the irradiation dose that caused 99.5% mortality of larvae was 254 Gy (220-289 Gy) and 99.9968% mortality of the 3rd instars was 267 Gy (184-351 Gy) with 95% fiducial limits. The mortality of litchi stem-end borer increased when the irradiation treatment was coupled with extended cool storage (76 deg. C). Hatching of eggs was decreased with increasing dosages to the eggs of 250-600 Gy. Prepupae treated at 300-600 Gy were more radiosensitive than 4-5 days old pupae. The results of visual quality observation indicated that the rotten fruit rate of litchi fruits was reduced by 250-350 Gy treatment. (author)

Fungi and minerals occurring in heartwood discolorations in Ouercus robur trees

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Krystyna Przybył

2011-01-01

Full Text Available The studied Quercus robur trees were oaks protected as monuments of nature (200-350 years old and oaks growing in forest stands (200-300 years old. The most visible symptoms occurring in aboveground organs included defoliation (25-75%, dying of twigs and branches. Discoloured heartwood, obtained with an 80-cm Pressler borer, differed in colour intensity from normal wood and was divided into two groups: stained brown and dark brown. The brown-stained heartwood was a more active site, considering the number of fungi and their ability to produce enzymes (phenoloxidase, pectinase and cellulase in comparison with the dark-brown-stained heartwood. This brown-stained heartwood contained also statistically more Ca, Mg and Mn.

Oak mortality associated with crown dieback and oak borer attack in the Ozark Highlands

Science.gov (United States)

Zhaofei Fan; John M. Kabrick; Martin A. Spetich; Stephen R. Shifley; Randy G. Jensen

2008-01-01

Oak decline and related mortality have periodically plagued upland oak–hickory forests, particularly oak species in the red oak group, across the Ozark Highlands of Missouri, Arkansas and Oklahoma since the late 1970s. Advanced tree age and periodic drought, as well as Armillaria root fungi and oak borer attack are believed to contribute to oak decline and mortality....

The parasitoids of the African white rice borer, Maliarpha separatella Ragonot (Lepidoptera: Pyralidae)

OpenAIRE

Polaszek, A.; Fitton, M.G.; Bianchi, G.; Huddleston, T.

2017-01-01

A key is provided for the recognition of the hymenopterous parasitoids of the African white rice borer, Maliarpha separatella Ragonot, a pest of rice in Africa and Madagascar. Five species are described as new: Braconidae: Chelonus maudae Huddleston, Rhacanotus carinafus Polaszek; Ichneumonidae: Prisfomerus bullis Fitton, Prisfomerus caris Fitton, Venturia jordanae Fitton. The following synonyms are proposed: Goniozus indicus Muesebeck, G. natalensis Gordh and G. procerae Risbec are synonymiz...

Management of stem borer (Chilo partellus Swinhoe in maize using conventional pesticides in Chitwan, Nepal

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Saraswati Neupane

2016-12-01

Full Text Available The stem borer (Chilo partellus Swinhoe is one of the most destructive pests of maize crop. Research experimentations were carried out on maize to control stem borer using conventional pesticides under field condition during summer season of two consecutive years from 2015 to 2016 at Rampur, Chitwan. All used pesticides had significant effect (P≤0.05 on percent damage and crop yield over control. In 2015, the lower percent damage (5.3% with higher crop yield (4.52 t ha-1 and lowest insect score (1.00 was observed in plot sprayed with spinosad 45% EC at 0.5 ml L-1 of water followed by plot treated with chloropyriphos 50% EC+cypermethrin 5%EC @1.5ml L-1 of water with percent damage of 6.60%, crop yield (4.23 t ha-1 and insect score of 1.60. Almost similar trend of insect incidence along with damage percentage and yield data were observed in 2016. The higher percent damage control (79.06% was observed at the plot sprayed after spinosad 45% EC at 0.5 ml L-1 of water with higher crop yield (4.58 t ha-1 and lowest insect score (1.00 followed by the plot treated with imidacloprid 17.8% @ 0.5 ml L-1 of water with percent damage control of 73.10 %, crop yield (3.38 t/ha and insect sore 1.50. The highest percent damage (20.63% was observed in the control plot with lower yield (0.95 t ha-1 and highest insect score (6.00. Over the years, spinosad 45% EC at 0.5 ml L-1 of water was effective bio-pesticide to control maize stem borer damage and also increase the yield.

Reproductive and developmental biology of the emerald ash borer parasitoid Spathius galinae (Hymenoptera: Braconidae) as affected by temperature

Science.gov (United States)

Emerald ash borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) is an invasive pest of serious concern in North America. To complement ongoing biological control efforts, Spathius galinae Belokobylskij and Strazenac (Hymenoptera: Braconidae), a recently-described specialist parasitoid of ...

Biological control of emerald ash borer in North America: current progress and potential for success

Science.gov (United States)

Jian J. Duan; Leah S. Bauer; Juli R. Gould; Jonathan P. Lelito

2012-01-01

The emerald ash borer (EAB) (Agrilus planipennis), a buprestid native to north-east Asia, was first discovered in North America near Detroit in 2002. EAB has since spread to at least 15 U.S. States and two Canadian provinces, threatening the existence of native ash trees (Fraxinus spp.). A classical biocontrol program was initiated...

Effects of modified atmosphere packaging on ripening of 'Douradão' peach related to pectolytic enzymes activities and chilling injury symptoms

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Ligia Regina Radomille de Santana

2011-12-01

Full Text Available The present study evaluated the effects of modified atmosphere packaging on inhibition of the development of chilling injury symptoms in 'Douradão' peach after cold storage and the possible involvement of cell wall enzymes. Fruits were harvested at the middle stadium of ripening, packed in polypropylene trays and placed inside low density polyethylene (LDPE bags (30, 50, 60 and 75 µm of thickness with active modified atmosphere (10 kPa CO2 + 1.5 kPa O2, balance N2. The following treatments were tested: Control: peaches held in nonwrapped trays; MA30: LDPE film - 30 µm; MA50: LDPE film - 50 µm; MA60: LDPE film - 60 µm and MA75: LDPE film - 75 µm. Fruits were kept at 1±1ºC and 90±5% relative humidity (RH for 28 days. After 14, 21 and 28 days, samples were withdrawn from MAP and kept in air at 25±1ºC and 90±5% RH for ripening. On the day of removal and after 4 days, peaches were evaluated for woolliness incidence, pectolytic enzymes activities. The respiratory rate and ethylene synthesis were monitored during 6 days of ripening. The results showed that MA50 and MA60 treatments had positive effect on the inhibition of the development of woolly texture and reduced pectin methylesterase activity on the ripe fruits, keeping good quality of 'Douradão' peach during 28 days of cold storage. The treatments Control, MA30 and MA75 showed higher woolliness incidence and did not present marketable conditions after 14 days of cold storage.

Origin and Dispersal of Domesticated Peach Palm

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Charles R. Clement

2017-11-01

Full Text Available Peach palm (Bactris gasipaes Kunth is a Neotropical palm domesticated by Native Americans. Its domestication resulted in a set of landraces (var. gasipaes, some with very starchy fruit used for fermentation, others with an equilibrium of starch and oil used as snacks. Which of the three wild types (var. chichagui was involved and where the domestication process began are unclear, with three hypotheses under discussion: an origin in southwestern Amazonia; or in northwestern South America; or multiple origins. We reevaluate one of the wild types, defining it as the incipient domesticate, and then evaluate these hypotheses using the Brazilian peach palm Core Collection and selected herbaria samples to: (1 model the potential distributions of wild and domesticated populations; (2 identify the probable origin of domestication with a phylogeographic analysis of chloroplast DNA sequences; and (3 determine the dispersal routes after domestication using spatial analysis of genetic diversity based on 17 nuclear microsatellite loci. The two very small-fruited wild types have distinct distributions in the northern Andes region and across southern Amazonia, both under moderately humid climates, while the incipient domesticate, partly sympatric with the southern wild type, is also found along the Equatorial Andes, in a more humid climatic envelope, more similar to that of the domesticated landraces. Two distribution models for Last Glacial Maximum conditions (CCSM4, MIROC also suggest distinct distributions for the two wild populations. The chloroplast DNA phylogeographic network confirms the area of sympatry of the incipient domesticate and the southern wild type in southwestern Amazonia as the origin of domestication. The spatial patterns of genetic diversity confirm the proposal of two dispersals, one along the Ucayali River, into western Amazonia, northwestern South America and finally Central America; the other along the Madeira River into central and

Indirect effects of emerald ash borer-induced ash mortality and canopy gap formation on epigaeic beetles.

Science.gov (United States)

Gandhi, Kamal J K; Smith, Annemarie; Hartzler, Diane M; Herms, Daniel A

2014-06-01

Exotic herbivorous insects have drastically and irreversibly altered forest structure and composition of North American forests. For example, emerald ash borer (Agrilus planipennis Fairmaire) from Asia has caused wide-scale mortality of ash trees (Fraxinus spp.) in eastern United States and Canada. We studied the effects of forest changes resulting from emerald ash borer invasion on epigaeic or ground beetles (Coleoptera: Carabidae) along a gradient of ash dieback and gap sizes in southeastern Michigan. Ground beetles were sampled in hydric, mesic, and xeric habitats in which black (Fraxinus nigra Marshall), green (Fraxinus pennsylvanica Marshall), and white (Fraxinus americana L.) ash were the most common species, respectively. During 2006-2007, we trapped 2,545 adult ground beetles comprising 52 species. There was a negative correlation between percent ash tree mortality in 2006 and catches of all beetles. Catches of Agonum melanarium Dejean (in 2006) and Pterostichus mutus (Say) (in 2006-2007) were negatively correlated with tree mortality and gap size, respectively. However, catches of Pterostichus corvinus Dejean were positively correlated with gap size in 2006. As ash mortality and average gap size increased from 2006 to 2007, catches of all beetles as well as P. mutus and Pterostichus stygicus (Say) increased (1.3-3.9 times), while species diversity decreased, especially in mesic and xeric stands. Cluster analysis revealed that beetle assemblages in hydric and mesic stand diverged (25 and 40%, respectively) in their composition from 2006 to 2007, and that hydric stands had the most unique beetle assemblages. Overall, epigaeic beetle assemblages were altered in ash stands impacted by emerald ash borer; however, these impacts may dissipate as canopy gaps close.

Phytohormone Signaling of the Resistance to Plum pox virus (PPV, Sharka Disease) Induced by Almond (Prunus dulcis (Miller) Webb) Grafting to Peach (P. persica L. Batsch).

Science.gov (United States)

Dehkordi, Azam Nikbakht; Rubio, Manuel; Babaeian, Nadali; Albacete, Alfonso; Martínez-Gómez, Pedro

2018-05-03

Plum pox virus (PPV, sharka) is a limiting factor for peach production, and no natural sources of resistance have been described. Recent studies, however, have demonstrated that grafting the almond cultivar "Garrigues" onto the "GF305" peach infected with Dideron-type (PPV-D) isolates progressively reduces disease symptoms and virus accumulation. Furthermore, grafting "Garrigues" onto "GF305" prior to PPV-D inoculation has been found to completely prevent virus infection, showing that resistance is constitutive and not induced by the virus. To unravel the phytohormone signaling of this mechanism, we analyzed the following phytohormones belonging to the principal hormone classes: the growth-related phytohormones cytokinin trans-zeatin (tZ) and the gibberellins GA₃ and GA₄; and the stress-related phytohormones ethylene acid precursor 1-aminocyclopropane-1-carboxylic acid (ACC), abscisic acid (ABA), salicylic acid (SA), and jasmonic acid (JA). PPV inoculation produced a significant increase in GA₃ and ABA in peach, and these imbalances were related to the presence of chlorosis symptoms. However, grafting "Garrigues" almond onto the PPV-inoculated "GF305" peach produced the opposite effect, reducing GA₃ and ABA contents in parallel to the elimination of symptoms. Our results showed the significant implication of SA in this induced resistance in peach with an additional effect on tZ and JA concentrations. This SA-induced resistance based in the decrease in symptoms seems to be different from Systemic Acquired Resistance (SAR) and Induced Systemic Resistance (ISR), which are based in other reactions producing necrosis. Further studies are necessary, however, to validate these results against PPV-D isolates in the more aggressive Marcus-type (PPV-M) isolates.

Mercury adsorption of modified mulberry twig chars in a simulated flue gas.

Science.gov (United States)

Shu, Tong; Lu, Ping; He, Nan

2013-05-01

Mulberry twig chars were prepared by pyrolysis, steam activation and impregnation with H2O2, ZnCl2 and NaCl. Textural characteristics and surface functional groups were performed using nitrogen adsorption and FTIR, respectively. Mercury adsorption of different modified MT chars was investigated in a quartz fixed-bed absorber. The results indicated that steam activation and H2O2-impregnation can improve pore structure significantly and H2O2-impregnation and chloride-impregnation promote surface functional groups. However, chloride-impregnation has adverse effect on pore structure. Mercury adsorption capacities of impregnated MT chars with 10% or 30% H2O2 are 2.02 and 1.77 times of steam activated MT char, respectively. Mercury adsorption capacity of ZnCl2-impregnated MT char increase with increasing ZnCl2 content and is better than that of NaCl-impregnated MT char at the same chloride content. The modified MT char (MT873-A-Z5) prepared by steam activation following impregnation with 5% ZnCl2 exhibits a higher mercury adsorption capacity (29.55 μg g(-1)) than any other MT chars. Copyright © 2013 Elsevier Ltd. All rights reserved.

Assessment of Trichogramma japonicum and T. chilonis as Potential Biological Control Agents of Yellow Stem Borer in Rice

Science.gov (United States)

Tang, Rui; Babendreier, Dirk; Zhang, Feng; Kang, Min; Song, Kai; Hou, Mao-Lin

2017-01-01

Two species of Trichogramma wasps were assessed for their effectiveness against yellow stem borer Scirpophaga incertulas. A laboratory cage test with T. japonicum and T. chilonis showed that both species parasitized yellow stem borer egg masses at 60.0% ± 9.13% and 40.7% ± 7.11%, respectively, with egg parasitism rates of 15.8% ± 22.2% for T. japonicum and 2.8% ± 5.0% for T. chilonis. Once the host eggs were parasitized, emergence rates were high for both species (95.7% ± 0.12% for T. japonicum and 100% for T. chilonis). In paddy field trials, the two Trichogramma species were released at three densities (50,000/ha, 100,000/ha and 200,000/ha) in Southwestern China. Egg mass parasitism was 9% ± 7.7% for T. japonicum and 15% ± 14.1% for T. chilonis, and again only a relatively small fraction of eggs was successfully parasitized. No clear conclusion could be drawn on the most efficient release rate as no significant differences were found among the three release rates. A comparison of field-collected T. japonicum with T. japonicum and T. chilonis mass reared on Corcyra cephalonica showed significantly larger body size and ovipositor length in field-collected wasps, suggesting potentially higher effectiveness on yellow stem borer eggs after at least one generation on the target host. Factors contributing to the low field parasitism rates are discussed. PMID:28208706

Internal papillomatosis with intrahepatic cholangiocarcinoma and gastrointestinal adenocarcinoma in a peach-fronted conure (Aratinga aurea).

Science.gov (United States)

Gibbons, Paul M; Busch, Martin D; Tell, Lisa A; Graham, Jennifer E; Lowenstine, Linda J

2002-01-01

A 17-yr-old pet female peach-fronted conure (Aratinga aurea) was presented with the chief complaints of mild lethargy and weight loss with increased appetite. Antemortem diagnostics included complete blood count, plasma biochemistry, and radiography. Abnormal findings included elevated inflammatory parameters (hyperfibrinogenemia) and a space-occupying mass in the region of the liver. Histologic examination of a liver biopsy sample indicated bile duct hyperplasia leading to a presumptive diagnosis of hepatoxicosis. The bird initially showed moderate improvement with supportive care, but its condition declined 9 days after the liver biopsy. Supportive care was attempted a second time, but the bird did not improve and euthanasia was elected. Abnormal gross necropsy findings were confined to the liver, which contained multiple tan nodules that exuded yellowish fluid on cut section. Histopathologic examination revealed multicentric bile duct hyperplasia and cholangiocarcinoma as well as segmental papillary hyperplasia and adenocarcinoma in the proventriculus, ventriculus, and throughout the intestinal tract. This is the first report of concurrent internal papillomatosis, gastrointestinal adenocarcinoma, and cholangiocarcinoma in a peach-fronted conure.

RETRAN-3D MOD003 Peach Bottom Turbine Trip 2 Multidimensional Kinetics Analysis Models and Results

International Nuclear Information System (INIS)

Mori, Michitsugu; Ogura, Katsunori; Gose, Garry C.; Wu, J.-Y.

2003-01-01

An analysis of the Peach Bottom Unit 2 Turbine Trip Test 2 (PB2/TT2) has been performed using RETRAN-3D MOD003. The purpose of the analysis was to investigate the PB2/TT2 overpressurization transient using the RETRAN-3D multidimensional kinetics model

Towards the development of an autocontamination trap system to manage populations of emerald ash borer (Coleoptera: Buprestidae) with the native entomopathogenic fungus, Beauveria bassiana.

Science.gov (United States)

Lyons, D Barry; Iavallée, Robert; Kyei-Poku, George; Van Frankenhuyzen, Kees; Johny, Shajahan; Guertin, Claude; Francese, Joseph A; Jones, Gene C; Blais, Martine

2012-12-01

Emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) is an invasive species from Asia that was discovered in North America Canada, in 2002. Herein, we describe studies to develop an autocontamination trapping system to disseminate Beauveria bassiana to control beetle populations. The standard trap for emerald ash borer in Canada is a light green prism trap covered in an insect adhesive and baited with (Z)-3-hexenol. We compared of green multifunnel traps, green intercept panel traps (both with and without fluon coating) and green prism traps for capturing emerald ash borer in a green ash plantation. The coated green multifunnel traps captured significantly more males and more females than any other trap design. We examined the efficacy of two native B. bassiana isolates, INRS-CFL and L49-1AA. In a field experiment the INRS-CFL isolate attached to multifunnel traps in autocontamination chambers retained its pathogenicity to emerald ash borer adults for up to 43 d of outdoor exposure. Conidia germination of the INRS-CFL isolate was >69% after outdoor exposure in the traps for up to 57 d. The L49-1AA isolate was not pathogenic in simulated trap exposures and the germination rate was extremely low (<5.3%). Mean (+/- SEM) conidia loads on ash borer adults after being autocontaminated in the laboratory using pouches that had been exposed in traps out of doors for 29 d were 579,200 (+/- 86,181) and 2,400 (+/- 681) for the INRS-CFL and the LA9-1AA isolates, respectively. We also examined the fungal dissemination process under field conditions using the L49-1AA isolate in a green ash plantation. Beetles were lured to baited green multifunnel traps with attached autocontamination chambers. Beetles acquired fungal conidia from cultures growing on pouches in the chambers and were recaptured on Pestick-coated traps. In total, 2,532 beetles were captured of which 165 (6.5%) had fungal growth that resembled B. bassiana. Of these 25 beetles were positive for

Risk-based selection of SSCs at Peach Bottom

International Nuclear Information System (INIS)

Krueger, G.A.; Marie, A.J.

1993-01-01

The purpose of identifying risk significant systems, structures, and components (SSCS) that are within the scope of the maintenance rule is to bring a higher level of attention to a subset of those SSCS. These risk-significant SSCs will have specific performance criteria established for them, and failure to meet this performance criteria will result in establishing goals to ensure the necessary improvement in performance. The Peach Bottom individual plant examination (IPE) results were used to provide insights for the verification of proposed probabilistic risk assessment (PRA) methods set forth in the Industry Maintenance Guidelines for Implementation of the Maintenance Rule. The objective of reviewing the methods for selection of SSCs that are considered risk significant was to ensure the methods used are logical, reproducible, and can be consistently applied

Karnyothrips flavipes, a previously unreported predatory thrips of the coffee berry borer: DNA-based gut content analysis

Science.gov (United States)

A new predator of the coffee berry borer, Hypothenemus hampei, was found in the coffee growing area of Kisii in Western Kenya. Field observations, laboratory trials and gut content analysis using molecular tools have confirmed the role of the predatory thrips Karnyothrips flavipes Jones (Phlaeothrip...

Dendrochronological reconstruction of the epicenter and early spread of emerald ash borer in North America

Science.gov (United States)

Nathan W. Siegert; Deborah G. McCullough; Andrew M. Liebhold; Frank W. Telewski

2014-01-01

Emerald ash borer Agrilus planipennis was identified in 2002 as the cause of extensive ash (Fraxinus spp.) decline and mortality in Detroit, Michigan, and has since killed millions of ash trees in the US and Canada. When discovered, it was not clear how long it had been present or at what location the invading colony started....

Role of emerald ash borer (Coleoptera: Buprestidae) larval vibrations in host-quality assessments by Tetrastichus planipennisi (Hymenoptera: Eulophidae)

Science.gov (United States)

Michael D. Ulyshen; Richard W. Mankin; Yigen Chen; Jian J. Duan; Therese M. Poland; Leah S. Bauer

2011-01-01

The biological control agent Tetrastichus planipennisi Yang (Hymenoptera: Eulophidae) is a gregarious larval endoparasitoid of the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), an invasive cambium-feeding species responsible for recent, widespread mortality of ash (Fraxinus spp.) in...

Goldspotted oak borer in California: Invasion history, biology, impact, management, and implications for Mediterranean forests worldwide

Science.gov (United States)

Tom Coleman; Steven Seybold

2016-01-01

In 2008, the goldspotted oak borer, Agrilus auroguttatus Schaeffer (Coleoptera: Buprestidae), was fi rst linked to elevated levels of oak mortality in southern California (CA), but it appears to have impacted oak woodlands and mixed conifer forests across all land ownerships in this region for nearly two decades. This unexpectedly damaging...

In vitro establishment of the hybrid rootstock ‘Garfi x Nemared’ (Garnem for peach

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Limberg Guevara Salguero

2016-04-01

Full Text Available The interspecific hybrid between almond and peach, ‘Garfield x Nemared’ (Prunus dulcis (Mill D.A.Webb x Prunus persica (L. Batsch. has become very important as rootstocks for peach in Bolivia, but propagation by traditional methods of this hybrid has been very difficult. In the present study the aim was to in vitro establishment of this hybrid. As initial explants, nodal segments from mother plants, growing under controlled culture conditions, were used. For disinfection two concentrations of sodium hypochlorite (0.5 and 0.75% and time (10 and 12 min were tested. The greatest percentage of establishment was achieved using 0.75% NaClO for 12 min in an MS culture medium free of growth regulators. A 100% control of the phenols oxidation was achieved with the combination of mother plants growing under 50% shade, young buds, use of 150 mg l-1 citric acid at the end of the disinfection process and into the culture medium and then place the test tubes with the nodal segments one week in the dark.   Keywords: interspecific hybrid, Prunus, tissue culture

Kaempferol glycosides from the twigs of Cinnamomum osmophloeum and their nitric oxide production inhibitory activities.

Science.gov (United States)

Lin, Huan-You; Chang, Shang-Tzen

2012-12-15

In the present study, ethanolic extract of twigs from Cinnamomum osmophloeum led to isolate nine kaempferol glycosides including two new kaempferol triglycosides that were characterized as kaempferol 3-O-β-D-xylopyranosyl-(1→2)-α-L-arabinofuranosyl-7-O-α-L-rhamnopyranoside (1) and kaempferol 3-O-β-D-xylopyranosyl-(1→2)-α-L-rhamnopyranosyl-7-O-α-L-rhamnopyranoside (2). The structures of these compounds were assigned by the application of 1D and 2D NMR spectroscopy and other techniques. Among these nine compounds, kaempferol 7-O-α-L-rhamnopyranoside (9) revealed inhibitory effect against LPS-induced production of nitric oxide in RAW 264.7 macrophages with an IC(50) value of 41.2 μM. It also slightly reduced PGE(2) accumulation by 26% at the concentration of 50 μM. Copyright © 2012 Elsevier Ltd. All rights reserved.

Differential transcript abundance and genotypic variation of four putative allergen-encoding gene families in melting peach

NARCIS (Netherlands)

Yang, Z.; Ma, Y.; Chen, L.; Xie, R.; Zhang, X.; Zhang, B.; Lu, M.; Wu, S.; Gilissen, L.J.W.J.; Ree, van R.; Gao, Z.

2011-01-01

We analysed the temporal and spatial transcript expression of the panel of 18 putative isoallergens from four gene families (Pru p 1–4) in the peach fruit, anther and leaf of two melting cultivars, to gain insight into their expression profiles and to identify the key family members. Genotypic

Exploratory survey for the emerald ash borer, Agrilus planipennis (Coleoptera: Buprestidae), and its natural enemies in China.

Science.gov (United States)

Houping Liu; Leah S. Bauer; Ruitong Gao; Tonghai Zhao; Toby R. Petrice; Robert A. Haack

2003-01-01

An exploratory survey for the emerald ash borer, Agrilus planipennis, and its natural enemies was conducted in China during October and November 2003. We examined 29 field plots in six provinces. We visually inspected living Fraxinus chinensis, F. mandshurica, F. pennsylvanica, F. rhynchophylla, and F. velutina...

Peach Bottom Turbine Trip Simulations with RETRAN Using INER/TPC BWR Transient Analysis Method

International Nuclear Information System (INIS)

Kao Lainsu; Chiang, Show-Chyuan

2005-01-01

The work described in this paper is benchmark calculations of pressurization transient turbine trip tests performed at the Peach Bottom boiling water reactor (BWR). It is part of an overall effort in providing qualification basis for the INER/TPC BWR transient analysis method developed for the Kuosheng and Chinshan plants. The method primarily utilizes an advanced system thermal hydraulics code, RETRAN02/MOD5, for transient safety analyses. Since pressurization transients would result in a strong coupling effect between core neutronic and system thermal hydraulics responses, the INER/TPC method employs the one-dimensional kinetic model in RETRAN with a cross-section data library generated by the Studsvik-CMS code package for the transient calculations. The Peach Bottom Turbine Trip (PBTT) tests, including TT1, TT2, and TT3, have been successfully performed in the plant and assigned as standards commonly for licensing method qualifications for years. It is an essential requirement for licensing purposes to verify integral capabilities and accuracies of the codes and models of the INER/TPC method in simulating such pressurization transients. Specific Peach Bottom plant models, including both neutronics and thermal hydraulics, are developed using modeling approaches and experiences generally adopted in the INER/TPC method. Important model assumptions in RETRAN for the PBTT test simulations are described in this paper. Simulation calculations are performed with best-estimated initial and boundary conditions obtained from plant test measurements. The calculation results presented in this paper demonstrate that the INER/TPC method is capable of calculating accurately the core and system transient behaviors of the tests. Excellent agreement, both in trends and magnitudes between the RETRAN calculation results and the PBTT measurements, shows reliable qualifications of the codes/users/models involved in the method. The RETRAN calculated peak neutron fluxes of the PBTT

Phytohormone Signaling of the Resistance to Plum pox virus (PPV, Sharka Disease) Induced by Almond (Prunus dulcis (Miller) Webb) Grafting to Peach (P. persica L. Batsch)

OpenAIRE

Azam Nikbakht Dehkordi; Manuel Rubio; Nadali Babaeian; Alfonso Albacete; Pedro Martínez-Gómez

2018-01-01

Plum pox virus (PPV, sharka) is a limiting factor for peach production, and no natural sources of resistance have been described. Recent studies, however, have demonstrated that grafting the almond cultivar “Garrigues” onto the “GF305” peach infected with Dideron-type (PPV-D) isolates progressively reduces disease symptoms and virus accumulation. Furthermore, grafting “Garrigues” onto “GF305” prior to PPV-D inoculation has been found to completely prevent virus infection, showing that resista...

Phytohormone Signaling of the Resistance to Plum pox virus (PPV, Sharka Disease Induced by Almond (Prunus dulcis (Miller Webb Grafting to Peach (P. persica L. Batsch

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Azam Nikbakht Dehkordi

2018-05-01

Full Text Available Plum pox virus (PPV, sharka is a limiting factor for peach production, and no natural sources of resistance have been described. Recent studies, however, have demonstrated that grafting the almond cultivar “Garrigues” onto the “GF305” peach infected with Dideron-type (PPV-D isolates progressively reduces disease symptoms and virus accumulation. Furthermore, grafting “Garrigues” onto “GF305” prior to PPV-D inoculation has been found to completely prevent virus infection, showing that resistance is constitutive and not induced by the virus. To unravel the phytohormone signaling of this mechanism, we analyzed the following phytohormones belonging to the principal hormone classes: the growth-related phytohormones cytokinin trans-zeatin (tZ and the gibberellins GA3 and GA4; and the stress-related phytohormones ethylene acid precursor 1-aminocyclopropane-1-carboxylic acid (ACC, abscisic acid (ABA, salicylic acid (SA, and jasmonic acid (JA. PPV inoculation produced a significant increase in GA3 and ABA in peach, and these imbalances were related to the presence of chlorosis symptoms. However, grafting “Garrigues” almond onto the PPV-inoculated “GF305” peach produced the opposite effect, reducing GA3 and ABA contents in parallel to the elimination of symptoms. Our results showed the significant implication of SA in this induced resistance in peach with an additional effect on tZ and JA concentrations. This SA-induced resistance based in the decrease in symptoms seems to be different from Systemic Acquired Resistance (SAR and Induced Systemic Resistance (ISR, which are based in other reactions producing necrosis. Further studies are necessary, however, to validate these results against PPV-D isolates in the more aggressive Marcus-type (PPV-M isolates.

The spatial genetic differentiation of the legume pod borer, Maruca vitrata F. (Lepidoptera: Crambidae) populations in West Africa

KAUST Repository

Agunbiade, Tolulope A.; Coates, Brad Steven; Kim, Kyungseok; Forgacs, D.; Margam, Venu; Murdock, Larry L.; Ba, Malick N.; Binso-Dabiré , Clé mentine L.; Baoua, Ibrahim B.; Ishiyaku, Mohammad F.; Tamò , Manuele; Pittendrigh, Barry Robert

2012-01-01

The legume pod borer, Maruca vitrata, is an endemic insect pest that causes significant yield loss to the cowpea crop in West Africa. The application of population genetic tools is important in the management of insect pests but such data on M

ADVANCES IN PEACH, NECTARINE AND PLUM PROPAGATION

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NEWTON ALEX MAYER

2017-12-01

Full Text Available ABSTRACT Nursery trees of stone fruits (Prunus spp. are traditionally produced by union of two distinct genotypes - the rootstock and the scion - which, by grafting, form a composite plant that will be maintained throughout of all plant life. In Brazil, the rootstocks are predominantly seed propagated and therefore usually results in heterogeneous trees for vigor and edaphic adaptation. However, with advances in rootstock breeding programs that released cultivars and certification in several countries (notably in Europe, the system will come gradually evolving for vegetative propagation (cuttings and tissue culture and use of seeds of selected rootstocks with specific characteristics and potted nursery trees production. For scion cultivar propagation, the budding system (with its many variations has predominantly been adopted in major producing countries. This review had as objective to comment main propagation methods adopted for rootstocks and scion in peach, nectarine and plum, and recent technical progress obtained as well as the needs of improvement for nursery tree production.

76 FR 31888 - Nectarines and Fresh Peaches Grown in California; Termination of Marketing Order 916 and the...

Science.gov (United States)

2011-06-02

... orders also authorize production research and marketing research and development projects, as well as the... DEPARTMENT OF AGRICULTURE Agricultural Marketing Service 7 CFR Parts 916 and 917 [Doc. No. AMS-FV... Marketing Order 916 and the Peach Provisions of Marketing Order 917 AGENCY: Agricultural Marketing Service...

Ecology of the cocoa pod borer, Conopomorpha cramerella (Lepidoptera: Gracillariidae), a major pest for the cocoa industry

Science.gov (United States)

Conopomorpha cramerella, the cocoa pod borer (CPB), has been known to damage cocoa pods for more than 100 years, but information on the ecology of this species is scant in the scientific literature. That which does exist is scattered in obscure local journals, not readily accessible, and often unve...

Emerald ash borer biocontrol in ash saplings: the potential for early stage recovery of North American ash

Science.gov (United States)

In many parts of North America, ash stands have been reduced by the emerald ash borer (Agrilus planipennis) invasion to a few surviving mature trees and young basal sprouts, saplings, and seedlings. Without a seed bank, ash tree recovery will require survival and maturation of these younger cohorts...

Progress and challenges of protecting North American ash trees from the emerald ash borer using biological control

Science.gov (United States)

Jian Duan; Leah Bauer; Roy van Driesche; Juli. Gould

2018-01-01

After emerald ash borer (EAB), Agrilus planipennis Fairmaire, was discovered in the United States, a classical biological control program was initiated against this destructive pest of ash trees (Fraxinus spp.). This biocontrol program began in 2007 after federal regulatory agencies and the state of Michigan approved release of...

Factors affecting the survival of ash (Fraxinus spp.) trees infested by emerald ash borer (Agrilus planipennis)

Science.gov (United States)

Kathleen S. Knight; John P. Brown; Robert P. Long

2013-01-01

Emerald ash borer (Agrilus planipennis) (EAB), an Asian woodboring beetle accidentally introduced in North America, has killed millions of ash (Fraxinus spp.) trees and is spreading rapidly. This study examined the effects of tree- and site-level factors on the mortality of ash trees in stands infested by EAB in OH, USA. Our data...

Use of unwounded ash trees for the detection of emerald ash borer adults: EAB landing behavior

Science.gov (United States)

Jordan M. Marshall; Melissa J. Porter; Andrew J. Storer

2011-01-01

Incorporation of multiple trapping techniques and sites within a survey program is essential to adequately identify the range of emerald ash borer (EAB) (Agrilus planipennis Fairmaire) infestation. Within natural forests, EAB lands on stick band traps wrapped around girdled ash trees at a rate similar to that on unwounded ash trees. The objective of...

Phytosanitary irradiation of peach fruit moth (Lepidoptera: Carposinidae) in apple fruits

Science.gov (United States)

Zhan, Guoping; Li, Baishu; Gao, Meixu; Liu, Bo; Wang, Yuejin; Liu, Tao; Ren, Lili

2014-10-01

Peach fruit moth, Carposina sasakii Matsumura, is a serious pest of many pome and stone fruits and presents a quarantine problem in some export markets. It is widely distributed in pome fruit production areas in China, Japan, Korea, North Korea and the Far Eastern Federal District of Russia. In this investigation, gamma radiation dose-response tests were conducted with late eggs (5-d-old) and various larval stages, followed by large-scale confirmatory tests on the most tolerant stage in fruit, the fifth instar. The dose-response tests, with the target radiation dose of 20 (late eggs), 40, 60, 80, 100, 120, 140, and 160 Gy (late fifth instars in vitro) respectively applied to all stages, showed that the tolerance to radiation increased with increasing age and developmental stage. The fifth instar (most advanced instar in fruits) was determined to be the most tolerant stage requiring an estimated minimum absorbed dose of 208.6 Gy (95% CI: 195.0, 226.5 Gy) to prevent adult emergence at 99.9968% efficacy (95% confidence level). In the confirmatory tests, irradiation was applied to 30,850 late fifth instars in apple fruits with a target dose of 200 Gy (171.6-227.8 Gy measured), but only 4 deformed adults emerged that died 2 d afterwards without laying eggs. A dose of 228 Gy may be recommended as a phytosanitary irradiation treatment under ambient atmosphere for the control of peach fruit moth on all commodities with an efficacy of 99.9902% at 95% confidence level.

Alpha-amylase inhibitor-1 gene from Phaseolus vulgaris expressed in Coffea arabica plants inhibits alpha-amylases from the coffee berry borer pest.

Science.gov (United States)

Barbosa, Aulus E A D; Albuquerque, Erika V S; Silva, Maria C M; Souza, Djair S L; Oliveira-Neto, Osmundo B; Valencia, Arnubio; Rocha, Thales L; Grossi-de-Sa, Maria F

2010-06-17

Coffee is an important crop and is crucial to the economy of many developing countries, generating around US$70 billion per year. There are 115 species in the Coffea genus, but only two, C. arabica and C. canephora, are commercially cultivated. Coffee plants are attacked by many pathogens and insect-pests, which affect not only the production of coffee but also its grain quality, reducing the commercial value of the product. The main insect-pest, the coffee berry borer (Hypotheneumus hampei), is responsible for worldwide annual losses of around US$500 million. The coffee berry borer exclusively damages the coffee berries, and it is mainly controlled by organochlorine insecticides that are both toxic and carcinogenic. Unfortunately, natural resistance in the genus Coffea to H. hampei has not been documented. To overcome these problems, biotechnological strategies can be used to introduce an alpha-amylase inhibitor gene (alpha-AI1), which confers resistance against the coffee berry borer insect-pest, into C. arabica plants. We transformed C. arabica with the alpha-amylase inhibitor-1 gene (alpha-AI1) from the common bean, Phaseolus vulgaris, under control of the seed-specific phytohemagglutinin promoter (PHA-L). The presence of the alpha-AI1 gene in six regenerated transgenic T1 coffee plants was identified by PCR and Southern blotting. Immunoblotting and ELISA experiments using antibodies against alpha-AI1 inhibitor showed a maximum alpha-AI1 concentration of 0.29% in crude seed extracts. Inhibitory in vitro assays of the alpha-AI1 protein against H. hampei alpha-amylases in transgenic seed extracts showed up to 88% inhibition of enzyme activity. This is the first report showing the production of transgenic coffee plants with the biotechnological potential to control the coffee berry borer, the most important insect-pest of crop coffee.

α-Amylase inhibitor-1 gene from Phaseolus vulgaris expressed in Coffea arabica plants inhibits α-amylases from the coffee berry borer pest

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Oliveira-Neto Osmundo B

2010-06-01

Full Text Available Abstract Background Coffee is an important crop and is crucial to the economy of many developing countries, generating around US$70 billion per year. There are 115 species in the Coffea genus, but only two, C. arabica and C. canephora, are commercially cultivated. Coffee plants are attacked by many pathogens and insect-pests, which affect not only the production of coffee but also its grain quality, reducing the commercial value of the product. The main insect-pest, the coffee berry borer (Hypotheneumus hampei, is responsible for worldwide annual losses of around US$500 million. The coffee berry borer exclusively damages the coffee berries, and it is mainly controlled by organochlorine insecticides that are both toxic and carcinogenic. Unfortunately, natural resistance in the genus Coffea to H. hampei has not been documented. To overcome these problems, biotechnological strategies can be used to introduce an α-amylase inhibitor gene (α-AI1, which confers resistance against the coffee berry borer insect-pest, into C. arabica plants. Results We transformed C. arabica with the α-amylase inhibitor-1 gene (α-AI1 from the common bean, Phaseolus vulgaris, under control of the seed-specific phytohemagglutinin promoter (PHA-L. The presence of the α-AI1 gene in six regenerated transgenic T1 coffee plants was identified by PCR and Southern blotting. Immunoblotting and ELISA experiments using antibodies against α-AI1 inhibitor showed a maximum α-AI1 concentration of 0.29% in crude seed extracts. Inhibitory in vitro assays of the α-AI1 protein against H. hampei α-amylases in transgenic seed extracts showed up to 88% inhibition of enzyme activity. Conclusions This is the first report showing the production of transgenic coffee plants with the biotechnological potential to control the coffee berry borer, the most important insect-pest of crop coffee.

Predation by Flat Bark Beetles (Coleoptera: Silvanidae and Laemophloeidae) on Coffee Berry Borer (Coleoptera: Curculionidae) in Hawaii coffee

Science.gov (United States)

Coffee berry borer(CBB), Hypothenemus hampei, is a serious pest of coffee worldwide and a new invasive pest in Hawaii. Adult flat bark beetles, mainly Leptophloeus sp.(75%) and Cathartus quadricollis(21%) (Coleoptera: Laemophloeidae and Silvanidae, respectively), were found feeding in CBB-infested c...

Effects of the emerald ash borer invasion on the community composition of arthropods associated with ash tree boles

Science.gov (United States)

Emerald ash borer (EAB), Agrilus planipennis Fairmaire is an invasive non-native wood-boring beetle that has killed hundreds of millions of ash trees (Fraxinus spp.) in North America, and threatens to extirpate the ecological services provided by the genus. Identifying the arthropod community assoc...

Development of a sprayable slow-release formulation for the sex pheromone of the Mediterranean Corn Borer, Sesamia nonagroides

NARCIS (Netherlands)

Vlieger, J.J. de

2008-01-01

In the FAIR project "Pheromaize", CT96-1302, the main objective is to provide European growers with a reliable, cost effective and environmentally friendly technology based on pest mating disruption. The project is mainly focused on Mediterranean Corn Borer (MCB), Sesamia nonagroides, the key pest

Characterization of Amazon fibers of the peach palm, balsa, and babassu by XDR, TGA and NMR; Caracterizacao das fibras amazonicas de pupunha, babacu e balsa atraves de DRX, TG e RMN

Energy Technology Data Exchange (ETDEWEB)

Martins, Maria A.; Marconcini, Jose M., E-mail: mariaalice@cnpdia.embrapa.br [Embrapa Instrumentacao, Sao Carlos-SP (Brazil); Morelli, Carolina L.; Marinelli, Alessandra L.; Bretas, Rosario E.S. [Universidade Federal de Sao Carlos - UFSCar, Sao Carlos, SP (Brazil)

2011-07-01

The aim of this work was to present the results by testing X-ray diffraction (XRD), thermogravimetric analysis (TG), nuclear magnetic resonance (NMR) and determining the moisture content of the peach palm, balsa and babassu fibers for assessing the feasibility of composite materials. The fibers of peach palm, balsa and babassu showed characteristic chemical structure of lignocellulosic material, and good thermal stability up to 220 deg C. The fiber with the highest crystallinity index (Ic) is the peach palm (72%) and the less crystalline is the babassu (37%), while the balsa fibers have Ic equal to 64%. The results have shown that these fibers can be used in the manufacture of composite materials. (author)

Mining microsatellites in the peach genome: development of new long-core SSR markers for genetic analyses in five Prunus species.

Science.gov (United States)

Dettori, Maria Teresa; Micali, Sabrina; Giovinazzi, Jessica; Scalabrin, Simone; Verde, Ignazio; Cipriani, Guido

2015-01-01

A wide inventory of molecular markers is nowadays available for individual fingerprinting. Microsatellites, or simple sequence repeats (SSRs), play a relevant role due to their relatively ease of use, their abundance in the plant genomes, and their co-dominant nature, together with the availability of primer sequences in many important agricultural crops. Microsatellites with long-core motifs are more easily scored and were adopted long ago in human genetics but they were developed only in few crops, and Prunus species are not among them. In the present work the peach whole-genome sequence was used to select 216 SSRs containing long-core motifs with tri-, tetra- and penta-nucleotide repeats. Microsatellite primer pairs were designed and tested for polymorphism in the five diploid Prunus species of economic relevance (almond, apricot, Japanese plum, peach and sweet cherry). A set of 26 microsatellite markers covering all the eight chromosomes, was also selected and used in the molecular characterization, population genetics and structure analyses of a representative sample of the five diploid Prunus species, assessing their transportability and effectiveness. The combined probability of identity between two random individuals for the whole set of 26 SSRs was quite low, ranging from 2.30 × 10(-7) in peach to 9.48 × 10(-10) in almond, confirming the usefulness of the proposed set for fingerprinting analyses in Prunus species.

Comparative RNA-seq analysis of early-infected peach leaves by the invasive phytopathogen Xanthomonas arboricola pv. pruni.

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Didier Socquet-Juglard

Full Text Available Xanthomonas arboricola pv. pruni is a quarantine bacterial pathogen that threatens peach production by causing necrotic spots on leaves and fruits, thus with the potential of severely reducing yields. The current understanding of the host plant defense responses to the pathogen is very limited. Using whole transcriptome sequencing, differential gene expression was analyzed at two time points, 2 h and 12 h post inoculation (hpi, by comparing the inoculated samples to their respective controls. On the total of 19,781 known peach genes that were expressed in all time points and conditions, 34 and 263 were differentially expressed at 2 and 12 hpi, respectively. Of those, 82% and 40% were up-regulated, respectively; and 18% and 60% were down-regulated, respectively. The functional annotation based on gene ontology (GO analysis highlighted that genes involved in metabolic process and response to stress were particularly represented at 2 hpi whereas at 12 hpi cellular and metabolic processes were the categories with the highest number of genes differentially expressed. Of particular interest among the differentially expressed genes identified were several pathogen-associated molecular pattern (PAMP receptors, disease resistance genes including several RPM1-like and pathogenesis related thaumatin encoding genes. Other genes involved in photosynthesis, in cell wall reorganization, in hormone signaling pathways or encoding cytochrome were also differentially expressed. In addition, novel transcripts were identified, providing another basis for further characterization of plant defense-related genes. Overall, this study gives a first insight of the peach defense mechanisms during the very early stages of infection with a bacterial disease in the case of a compatible interaction.

The Cloning and Functional Characterization of Peach CONSTANS and FLOWERING LOCUS T Homologous Genes PpCO and PpFT.

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Xiang Zhang

Full Text Available Flowering is an essential stage of plant growth and development. The successful transition to flowering not only ensures the completion of plant life cycles, it also serves as the basis for the production of economically important seeds and fruits. CONSTANS (CO and FLOWERING LOCUS T (FT are two genes playing critical roles in flowering time control in Arabidopsis. Through homology-based cloning and rapid-amplifications of cDNA ends (RACE, we obtained full-lengths cDNA sequences of Prunus persica CO (PpCO and Prunus persica FT (PpFT from peach (Prunus persica (L. Batsch and investigated their functions in flowering time regulation. PpCO and PpFT showed high homologies to Arabidopsis CO and FT at DNA, mRNA and protein levels. We showed that PpCO and PpFT were nucleus-localized and both showed transcriptional activation activities in yeast cells, consistent with their potential roles as transcription activators. Moreover, we established that the over-expression of PpCO could restore the late flowering phenotype of the Arabidopsis co-2 mutant, and the late flowering defect of the Arabidopsis ft-1 mutant can be rescued by the over-expression of PpFT, suggesting functional conservations of CO and FT genes in peach and Arabidopsis. Our results suggest that PpCO and PpFT are homologous genes of CO and FT in peach and they may function in regulating plant flowering time.

Evaluation of different doses of gamma radiation on physicochemical characteristics of peach Prunus persica (cv. Chimarrita) minimally processed

International Nuclear Information System (INIS)

Oliveira, Ana Claudia S.; Silva, Lucia C.A.S.; Perecin, Thalita Neme; Arthur, Valter; Harder, Marcia N.C.; Mansi, Debora N.; Canniatti-Brazaca, Solange G.

2009-01-01

The objective was to evaluate the effect of different doses of gamma radiation on the physico-chemical characteristics of peach Prunus persica (cv. Chimarrita) minimally processed, to increase the useful life of the fruit. The peaches were purchased at Ceasa of Campinas/SP and taken to the Laboratory of Radiobiology and Environment of CENA/USP (Piracicaba/SP), which were washed in tap water, peeled and cut into four pieces. The pieces of peach were dipped in sodium hypochlorite solution of 15 mL/L for 4 minutes and dry in a plastic support. Then it were placed in plastic containers (polypropylene). Subsequently, they were irradiated in a Cobalt-60 source, type Gammacell-220 (dose rate of 0,543 kGy/hour) with doses of: 0 (control), 1.0 and 2.0 kGy and stored at a temperature of 8 deg C. The experimental was developed entirely at random with 3 replicates for each treatment. For the statistic analysis was using the Tuckey test at 5% level of probability. Subsequently, analysis was carried out: color factors (l, a, b), pH, soluble solids (deg Brix), acidity and vitamin C. The tests were performed at 1, 3 and 6 days after irradiation. According to the results concluded that the analysis of color and acidity there was no significant difference between treatments, however, for the soluble solids (deg Brix), vitamin C and texture significant difference showing a decrease proportional to increasing doses of radiation and storage time. But the pH increased in relation to dose and during the analysis. (author)

Effect of gamma irradiation on refrigerator storage of peach fruits

International Nuclear Information System (INIS)

Kalinov, V.

1985-01-01

Peach fruits of cvs. Halle and Elberta were gamma irradiated by 0, 2.0, 2.5 and 3.0 kGy and stored in the usual way (20-25 grad C) or in refrigerator (5 and 0 grad C). The rate of mould decay, reduction in market quality and complex evaluation of the fruits at the time of storage were investigated. Linear regression correlations between the percentage of decay and the time of storage were estimated by probit-logarithmic transformation of the percentage of the decayed fruits. On that basis were assess the probable terms to reach 5% decay (permissible period of storage) and 10% decay. The criteria of quality and taste evaluation of the fruits for these periods were determined. The irradiation of peach fruits with 2.5 and 3.0 kGy prolonged 3 to 5 times the period free of decay (up to 5%) in the case of normal storage conditions as compared to the control (no irradiation). The sharp reduction in quality and the sensorial evaluation for these periods did not justify irradiation in case of usual storage conditions. Irradiation with the same rates in case of refrigeration storage proved a useful supplementary factor prolonging the periods before appearance of decay up to 17 days (i.e. 42% as compared to the control) and the permissible period of storage up to 25 days (25% more than the control). These results were obtained at a dose of 2.5 kGy for cv. Halle fruits, at dose 3.0 kGy for cv. Elberta fruits and at storage temperature of 0 grad C. For the periods up to 5% decay (25 to 26 days) the criteria of quality and the complex evaluation remained above the permissible level. Longer storage was not advisable because physiological damage of the fruit was evident

Developing rearing methods for Tetrastichus planipennisi (Hymenoptera: Eulophidae), a larval endoparasitoid of the emerald ash borer (Coleoptera: Buprestidae)

Science.gov (United States)

Philip Taylor; Jian J. Duan; Roger. Fuester

2011-01-01

Classical biological control efforts against emerald ash borer (EAB) (Agrilus planipennis Fairmaire) in North America primarily have focused on introduction and releases of exotic parasitoid species collected from northern parts of China. Recently, field surveys in Michigan, Pennsylvania, Ohio, and Ontario also indicate that some existing parasitoids...

Biological control of coffee berry borer: the role of DNA-based gut-content analysis in assessment of predation

Science.gov (United States)

The coffee berry borer, Hypothenemus hampei, is the most important pest of coffee worldwide, causing an estimated $500 million in damage annually. Infestation rates from 50-90% have been reported, significantly impacting coffee yields. Adult female H. hampei bore into the berry and lay eggs whose la...

Developing rearing methods for Tetrastichus planipennisi (Hymenoptera: Eulophidae), a larval endoparasitoid of the emerald ash borer (Coleoptera: Buprestidae)

Science.gov (United States)

Jian J. Duan; Mike Ulyshen; Leah Bauer; Ivich. Fraser

2011-01-01

Tetrastichus planipennisi Yong, a gregarious koinobiont endoparasitoid, is one of three hymenopteran parasitoids being released in the U.S. for biological control of the emerald ash borer (Agrilus planipennis Fairmair, EAB), an invasive beetle from Asia causing mortality of the ash trees (Fraxinus spp.) in North...

Survival of Lactobacillus rhamnosus probiotic strains in peach jam during storage at different temperatures

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Cinzia Lucia Randazzo

2013-12-01

Full Text Available The survival of six probiotic wild strains of Lactobacillus rhamnosus was compared with that of a type strain during 78 days of storage at 25 and 5 ºC in peach synthetic medium (PSM and commercial peach jam (PJ. Changes in viable cell counts, pH values, sugar content, and colour parameters were monitored. All strains exhibited better performances in PJ than in PSM, showing count values higher than 7 Log cfu g-1 up to 78 days of storage at 5 ºC. Almost all wild strains remained above the critical value of 6 Log cfu g-1 in samples stored at 25 ºC up to 45 days, while the Lb. rhamnosus GG type strain, used as control, was not able to survive later than 15 days. In the synthetic medium used, the strains showed better survival in the samples incubated at 25 ºC, remaining viable above the critical level up to 45 days of storage, except for the strain H12. The probiotic cultures added to jam did not significantly change the colour parameters of the product; however the metabolism of lactobacilli did cause changes in the pH and in the composition of sugars.

Can ash communities and their dependent species be partially protected through biological control of emerald ash borer

Science.gov (United States)

Ash trees were once relatively free of serious, major diseases and insect pests in North America until the arrival of the emerald ash borer (EAB), Agrilus planipennis Fairmaire, which was first detected in North America in Michigan in 2002 and has been detected in 32 U.S. states and two Canadian pro...

Assessment of maize stem borer damage on hybrid maize varieties in Chitwan, Nepal

OpenAIRE

Buddhi Bahadur Achhami; Santa Bahadur BK; Ghana Shyam Bhandari

2015-01-01

Maize is the second most important cereal crop in Nepal. However, national figure of grain production still remains below than the world's average grain production per unit area. Thus, this experiment was designed to determine the suitable time of maize planting, and to assess the peak period of one of the major insects, maize stem borer, in Chitwan condition. The results showed that plant damage percentage as per the maize planting month varies significantly, and the average plant damage per...

Evaluation of winter temperatures on apple budbreak using grafted twigs

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Fernando José Hawerroth

2013-09-01

Full Text Available Temperature is the main climate factor related to induction, maintenance and dormancy release in apple (Malus domestica Borkh.. The inadequate chilling exposure in apples causes budbreak problems, resulting in decrease in yield potential. Thus, the knowledge of physiological principles and environmental factors determining the dormancy phenomenon, especially winter temperature effects, it is necessary for the efficient selection of cultivars in a productive region. In addition, it is indispensable to adapt the orchard management aiming to decrease the problems caused by lack chilling during winter. The objective of this study was to evaluate the influence of different thermal conditions during the dormancy period on budbreak of apple cultivars. One-year-old twigs of 'Castel Gala' and 'Royal Gala' cultivars, grafted on M7 rootstock, were submitted to temperatures of 5, 10 and 15ºC for different exposure periods (168; 336; 672; 1,008 and 1,344 hours. After treatments execution, the plants were kept in a greenhouse at 25ºC. Budbreak was quantified when accumulated 3,444; 6,888; 10,332; 13,776; 17,220 and 20,664 GDHºC after temperature treatments. The cultivars responded differently to temperature effect during the winter period. The temperature of 15ºC during winter shows a greater effectiveness on 'Castel Gala' apple budbreak while in the 'Royal Gala' apples the temperatures of 5 and 10ºC show better performance. 'Castel Gala' cultivar (low chilling requirement may supply its physiological necessities, may be capable to budburst, even when subjected to higher temperatures in relation to 'Royal Gala' apples (high chilling requirement.

Mapping of quantitative trait loci for resistance to fall armyworm and southwestern corn borer leaf-feeding damage in maize.

Science.gov (United States)

Fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith), and southwestern corn borer (SWCB), Diatraea grandiosella Dyar are damaging insect pests of maize resulting in significant yield and economic losses. A previous study identified quantitative trait loci (QTL) that contribute to reduced leaf-fe...

Ionizing radiation as a phytosanitary treatment against European corn borer (Lepidoptera: Crambidae) in ambient, low oxygen, and cold conditions

Science.gov (United States)

The European corn borer, Ostrinia nubilalis (Hubner), (Lepidoptera: Crambidae), is a quarantine pest for several fresh commodities, including corn-on-the-cob, bell peppers, and green beans. Methyl bromide fumigation is the usual phytosanitary treatment, but the chemical is under increasing regulat...

PRODUCT NEEM AZAL T/S - BROAD-SPECTRUM PHYPOPESTICIDE FOR CONTROL OF PESTS ON VEGETABLE CROPS

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Vinelina Yankova

2016-09-01

Full Text Available Experiments for determination of the effectiveness of product Neem Azal T/S (a. i. azadirachtin were conducted at a concentration of 0,3% against some major pests in vegetable crops grown in greenhouses at the Maritsa Vegetable Crops research Institute, Plovdiv during the period 2010-2016. It was established very good insecticidal and acaricidal action of phytopesticide against: cotton aphid (Aphis gossypii Glov.; green peach aphid (Myzus persicae Sulz.; western flower trips (Frankliniella occidentalis Perg.; cotton bollworm (Helicoverpa armigera Hubn.; tomato borer (Tuta absoluta Meyrick and two-spotted spider mite (Tetranichus urticae Koch.. This product is a successful alternative to using chemical insecticides and acaricides.

Coast live oak, Quercus agrifolia, susceptibility and response to goldspotted oak borer, Agrilus auroguttatus, injury in southern California

Science.gov (United States)

Tom W. Coleman; Nancy E. Grulke; Miles Daly; Cesar Godinez; Susan L. Schilling; Philip J. Riggan; Steven J. Seybold

2011-01-01

Oak mortality is often associated with a complex of decline factors. We describe the morphological and physiological responses of coast live oak, Quercus agrifolia Née, in California to an invasive insect, the goldspotted oak borer (GSOB), Agrilus auroguttatus Schaeffer (Coleoptera: Buprestidae), and evaluate drought as a...

Modeling emerald ash borer dispersal using percolation theory: estimating the rate of range expansion in a fragmented landscape

Science.gov (United States)

Robin A. J. Taylor; Daniel A. Herms; Louis R. Iverson

2008-01-01

The dispersal of organisms is rarely random, although diffusion processes can be useful models for movement in approximately homogeneous environments. However, the environments through which all organisms disperse are far from uniform at all scales. The emerald ash borer (EAB), Agrilus planipennis, is obligate on ash (Fraxinus spp...

Microsatellite population genetics of the emerald ash borer (Agrilus planipennis Fairmaire): comparisons between Asian and North American populations

Science.gov (United States)

Carson C. Keever; Christal Nieman; Larissa Ramsay; Carol E. Ritland; Leah S. Bauer; D. Barry Lyons; Jenny S. Cory

2013-01-01

The emerald ash borer (EAB) (Agrilus planipennis Fairmaire) (Coleoptera; Buprestidae), is an invasive wood-boring beetle native to northeast Asia. This species was first detected in Michigan USA in 2002, and is a significant threat to native and ornamental ash tree species (Fraxinus spp.) throughout North America. We...

Pre-harvest application of calcium chloride and chitosan on fruit quality and storability of ‘Early Swelling’ peach during cold storage

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Ahmed Abdel Nabi Abdel Gayed

Full Text Available ABSTRACT Studies related with the storage of peach fruits have received great relevance in Egypt. In this study, the effect of pre-harvest sprays of calcium chloride and chitosan, separately and in combination, on quality attributes and storability of peach fruits stored at 0±1 °C was studied. ‘Early Swelling’ peach trees were sprayed twice with 1% or 2% calcium chloride. The first spraying was at pea stage, while the second one was performed at 10 days before harvesting. Chitosan sprays were performed at 0.5 or 1%, alone or in combination with 1 and 2% calcium chloride, at 10 days before harvesting. Untreated trees served as control. Fruits were harvested at maturity stage, then packaged and stored at 0±1 °C and 85-90% of relative humidity. Fruit physical and chemical properties were evaluated at 7-day intervals. Results showed that pre-harvest application with 2% CaCl2+1% chitosan was most effective in minimizing weight loss (% and decay (%, as well as in maintaining maximum firmness and lengthening shelf life. Fruit color was not affected by any of the treatments, while untreated fruits and calcium chloride treatment alone, at both applied concentrations, maintained higher total soluble solids (TSS, %, total phenolic content, and lower titratable acidity percentage.

Micro-CT unveils the secret life of the coffee berry borer (Hypothenemus hampei; Coleoptera, Curculionidae: Scolytinae) inside coffee berries

Science.gov (United States)

The coffee berry borer (Hypothenemus hampei (Ferrari); Coleoptera: Curculionidae: Scolytinae) is the most important insect pest of coffee worldwide, and due to the cryptic life habit of the insect inside coffee berries, effective pest management strategies have been difficult to develop. In this pap...

Assessment of engineering plant analyzer with Peach Bottom 2 stability tests

International Nuclear Information System (INIS)

Rohatgi, U.S.; Mallen, A.N.; Cheng, H.S.; Wulff, W.

1992-01-01

Engineering Plant Analyzer (EPA) has been developed to simulate plant transients for Boiling Water Reactor (BWR). Recently, this code has been used to simulate LaSalle-2 instability event which was initiated by a failure in the feed water heater. The simulation was performed for the scram conditions and for the postulated failure in the scram. In order to assess the capability of the EPA to simulate oscillatory flows as observed in the LaSalle event, EPA has been benchmarked with the available data from the Peach Bottom 2 (PB2) Instability tests PT1, PT2, and PT4. This document provides a description of these tests

Planning for and implementing an emerald ash borer-induced forest restoration program in municipal woodlands in Oakville, Ontario

Science.gov (United States)

Peter A. Williams; Candace. Karandiuk

2017-01-01

Oakville is an urban municipality with 846 ha of woodland. Management priorities are to maintain forest health, environmental health, and safety; wood production is a minor objective. The town developed a comprehensive strategy to plan for emerald ash borer (EAB; Agrilus planipennis) induced ash mortality and forest restoration. Oakville has begun...

Mites fluctuation population on peach tree (Prunus persica (L. Batsch and in associated plants

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Carla Rosana Eichelberger

2011-09-01

Full Text Available Despite the importance of peach (Prunus persica (L. Batsch in Rio Grande do Sul, little is known about mites fluctuation population considered important to this crop. The objective of this study was to know the population diversity and fluctuation of mite species associated with Premier and Eldorado varieties in Roca Sales and Venâncio Aires counties, Rio Grande do Sul. The study was conducted from July 2008 to June 2009 when 15 plants were randomly chosen in each area. The plants were divided in quadrants and from each one a branch was chosen from which three leaves were removed: one collected in the apical region, another in the medium and the other in the basal region, totalizing 180 leaves/area. Five of the most abundant associated plants were collected monthly in enough amounts for the screening under the stereoscopic microscope during an hour. A total of 1,124 mites were found belonging to 14 families and 28 species. Tetranychus ludeni Zacher, 1913, Panonychus ulmi (Koch, 1836 and Mononychellus planki (McGregor, 1950 were the most abundant phytophagous mites, whereas Typhlodromalus aripo Deleon, 1967 and Phytoseiulus macropilis (Banks, 1904 the most common predatory mites. The period of one hour under stereoscopic microscope was enough to get a representative sample. In both places evaluated the ecologic indices were low, but little higherin Premier (H' 0.56; EqJ: 0.43 when compared to Eldorado (H' 0.53; EqJ 0.40. In Premier constant species were not observed and accessory only Brevipalpus phoenicis (Geijskes, 1939, T. ludeni and T. aripo. Higher abundance was observed in December and January and bigger amount in April. Already in Eldorado, T. ludeni and P. ulmi were constants. Greater abundance was observed in November and December, whereas grater richness in December and January. In both orchards were not found mites in buds. Tetranychus ludeni is the most abundant phytophagous mites with outbreak population in November, December and

Disruption of Ethylene Responses by Turnip mosaic virus Mediates Suppression of Plant Defense against the Green Peach Aphid Vector.

Science.gov (United States)

Casteel, Clare L; De Alwis, Manori; Bak, Aurélie; Dong, Haili; Whitham, Steven A; Jander, Georg

2015-09-01

Plants employ diverse responses mediated by phytohormones to defend themselves against pathogens and herbivores. Adapted pathogens and herbivores often manipulate these responses to their benefit. Previously, we demonstrated that Turnip mosaic virus (TuMV) infection suppresses callose deposition, an important plant defense induced in response to feeding by its aphid vector, the green peach aphid (Myzus persicae), and increases aphid fecundity compared with uninfected control plants. Further, we determined that production of a single TuMV protein, Nuclear Inclusion a-Protease (NIa-Pro) domain, was responsible for changes in host plant physiology and increased green peach aphid reproduction. To characterize the underlying molecular mechanisms of this phenomenon, we examined the role of three phytohormone signaling pathways, jasmonic acid, salicylic acid, and ethylene (ET), in TuMV-infected Arabidopsis (Arabidopsis thaliana), with or without aphid herbivory. Experiments with Arabidopsis mutants ethylene insensitive2 and ethylene response1, and chemical inhibitors of ET synthesis and perception (aminoethoxyvinyl-glycine and 1-methylcyclopropene, respectively), show that the ET signaling pathway is required for TuMV-mediated suppression of Arabidopsis resistance to the green peach aphid. Additionally, transgenic expression of NIa-Pro in Arabidopsis alters ET responses and suppresses aphid-induced callose formation in an ET-dependent manner. Thus, disruption of ET responses in plants is an additional function of NIa-Pro, a highly conserved potyvirus protein. Virus-induced changes in ET responses may mediate vector-plant interactions more broadly and thus represent a conserved mechanism for increasing transmission by insect vectors across generations. © 2015 American Society of Plant Biologists. All Rights Reserved.

Short-Term Responses of Ground Beetles to Forest Changes Caused by Early Stages of Emerald Ash Borer (Coleoptera: Buprestidae)-Induced Ash Mortality.

Science.gov (United States)

Perry, Kayla I; Herms, Daniel A

2016-04-22

Emerald ash borer (Agrilus planipennis Fairmaire), an invasive wood-boring beetle native to Asia, has killed hundreds of millions of ash trees since its accidental introduction into North America, resulting in widespread formation of canopy gaps and accumulations of coarse woody debris (CWD) in forests. The objective was to quantify effects of canopy gaps and CWD caused by early stages of emerald ash borer-induced ash mortality, and their interaction on ground beetle assemblages. The impact of canopy gaps and CWD varied, as gaps affected beetle assemblages in 2011, while effects of CWD were only observed in 2012. Gaps decreased beetle activity-abundance, and marginally decreased richness, driving changes in species composition, but evenness and diversity were unaffected. Effects of the CWD treatment alone were minimal, but CWD interacted with the canopy treatment, resulting in an increase in activity-abundance of ground beetles in canopy gaps without CWD, and a marginal increase in species richness in canopy gaps with CWD. Although there were some initial changes in species composition, these were ephemeral, suggesting that ground beetle assemblages may be resilient to disturbance caused by emerald ash borer. This study contributes to our understanding of the cascading ecological impacts of biological invasions on forest ecosystems. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Microbial control of emerald ash borer, Agrilus planipennis (Coleoptera: Buprestidae) with Beauveria bassiana strain GHA: Greenhouse and field trials

Science.gov (United States)

Houping Liu; Leah S. Bauer

2008-01-01

In 2003-2004, the lethal and sublethal effects of Beauveria bassiana strain GHA on emerald ash borer, Agrilus planipennis (Coleoptera: Buprestidae) adults and larvae were evaluated using topical spray and fungal band treatments in the greenhouse and field. B. bassiana strain GHA was moderately effective against...

Distribution of trunk-injected 14C-imidacloprid in ash trees and effects on emerald ash borer (Coleoptera: Buprestidae) adults

Science.gov (United States)

David Mota-Sánchez; Bert M. Cregg; Deborah G. McCullough; Therese M. Poland; Robert M. Hollingworth

2009-01-01

The emerald ash borer (EAB), Agrilus planipennis (Coleoptera: Buprestidae) is a destructive exotic pest of North American ash (Fraxinus sp.) trees. Trunk injection of imidacloprid is commonly used to protect landscape ash trees from A. planipennis damage. Efficacy can vary and little is known about the...

Interaction between N-fertilizer and water availability on borer-rot complex in sugarcane

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Luiz Eduardo da Rocha Pannuti

2015-03-01

Full Text Available This study aimed to evaluate the effects of nitrogen availability in fertigation and rainfed management, as well as their interactions with the incidence of and damage caused by D. saccharalis and red rot in sugarcane. The experiment consisted of four treatments (0 and 150 kg ha–1 of N-fertilizer with irrigation; 0 and 150 kg ha–1 of N-fertilizer in rainfed management in a randomized complete block design with four replications. The evaluated parameters were the number of holes and internodes with red rot per meter of cultivation, stalk yield and sugar content. In the laboratory (T = 25 ± 2 °C; R.H. = 70 ± 10%: 12:12-L:D, we evaluated the attractiveness and consumption of fragments of stalks from the different treatments for fourth instar larvae through choice and no-choice tests in a randomized complete block design with ten replications. Nitrogen fertilization via irrigation has favorable effects on borer-rot complex and leads to higher gains in stalk and sugar yields when compared to rainfed management. The increments of stalk and sugar yields due to nitrogen fertilization compensates for the increase in borer-rot complex infestation. In laboratory tests, D. saccharalis larvae were similarly attracted to all treatments regardless of the doses of N-fertilizer or the water regimes evaluated. However, fragments of sugarcane stalks produced with nitrogen fertilization were consumed more by D. saccharalis in both water regimes.

A new species of genus Oobius (Hymenoptera: Encyrtidae) from the Russian Far East that parasitizes eggs of Emerald Ash Borer

Science.gov (United States)

A new egg parasitoid of the emerald ash borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) is described from the Vladivostok, Russia, Oobius primorskyensis Yao & Duan n. sp. Both morphological characters and analysis of DNA sequence divergence suggest that this species is different from t...

The parasites of cereal stem borers (Lepidoptera: Cossidae, Crambidae, Noctuidae, Pyralidae) in Africa, belonging to the family Braconidae (Hymenoptera: Ichneumonoidea)

NARCIS (Netherlands)

Achterberg, van C.; Polaszek, A.

1996-01-01

A review is given of the parasites (parasitoids) of the African cereal stem borers (including introduced species) belonging to the family Braconidae (Hymenoptera); 38 species belonging to 19 genera are keyed and treated. Three new species are described: Macrocentrus sesamivorus spec. nov. from

Effects of trap type, placement and ash distribution on emerald ash borer captures in a low density site

Science.gov (United States)

Deborah G. McCullough; Nathan W. Siegert; Therese M. Poland; Steven J. Pierce; Su Zie. Ahn

2011-01-01

Effective methods for early detection of newly established, low density emerald ash borer (Agrilus planipennis Fairmaire) infestations are critically needed in North America. We assessed adult A. planipennis captures on four types of traps in a 16-ha site in central Michigan. The site was divided into 16 blocks, each comprised of...

Evaluating the economic costs and benefits of slowing the spread of emerald ash borer in Ohio and Michigan

Science.gov (United States)

Jonathan Bossenbroek; Audra Croskey; David Finnoff; Louis Iverson; Shana M. McDermott; Anantha Prasad; Charles Sims; Davis. Sydnor

2015-01-01

The emerald ash borer (Agrilus planipennis; EAB) is poised to wipe out native ashes (Fraxinus spp.) in North America with expected catastrophic losses to ash tree forestry (MacFarlane and Meyer 2005). EAB was first discovered in Detroit in 2002. Most scientists hypothesize that it entered the United States through solid wood...

Can Emerald ash borer, Agrilus planipennis (Coleoptera: Buprestidae), emerge from logs two summers after infested trees are cut?

Science.gov (United States)

Toby R. Petrice; Robert A. Haack

2007-01-01

Emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is a serious invasive pest of ash trees (Fraxinus spp.) in North America. Much of EAB's range expansion has been attributed to human-assisted movement of infested items such as ash logs and firewood. It is unclear the amount of time that logs cut...

Micropropagation of ornamental Prunus spp. and GF305 peach, a Prunus viral indicator.

Science.gov (United States)

Kalinina, Anna; Brown, Daniel C W

2007-07-01

A micropropagation approach was developed for nine ornamental Prunus species, P. americana, P. cistena, P. glandulosa, P. serrulata 'Kwanzan', P. laurocerasus, P. sargentii, P. tomentosa, P. triloba, P. virginiana 'Schubert', commercially important in North America, and GF305 peach, commonly used for Prunus virus indexing. The micropropagation cycle based on proliferation of vegetative tissues includes establishment of tissue culture through introduction of shoot meristems in vitro, shoot proliferation, root induction and plant acclimatization steps and can be completed in 5 months. A meristem sterilization protocol minimized bacterial and fungal contamination. Multiple shoot formation in ornamental Prunus was obtained through the use of 1 mg l(-1) 6-benzyladenine. For GF305 peach, alteration in the sugar composition, fructose instead of sucrose, and addition of 1 mg l(-1 )ferulic acid had a significant impact on the shoot proliferation rate and maintenance of long-term in vitro culture. Rooting and plant acclimatization conditions were improved using a two-step protocol with a 4-day root induction in indole-3-butiric acid (IBA)-containing media with consequent 3-week root elongation in IBA-free media. One-month incubation of rooted shoots in a vermiculite-based medium resulted in additional shoot and root growth and provided better acclimatization and plant recovery. The micropropagation approach can be used for maintenance of the clonal properties for Prunus spp. as well as a protocol to support meristem therapy against viral infection.

Comparison of the genetic determinism of two key phenological traits, flowering and maturity dates, in three Prunus species: peach, apricot and sweet cherry.

Science.gov (United States)

Dirlewanger, E; Quero-García, J; Le Dantec, L; Lambert, P; Ruiz, D; Dondini, L; Illa, E; Quilot-Turion, B; Audergon, J-M; Tartarini, S; Letourmy, P; Arús, P

2012-11-01

The present study investigates the genetic determinism of flowering and maturity dates, two traits highly affected by global climate change. Flowering and maturity dates were evaluated on five progenies from three Prunus species, peach, apricot and sweet cherry, during 3-8 years. Quantitative trait locus (QTL) detection was performed separately for each year and also by integrating data from all years together. High heritability estimates were obtained for flowering and maturity dates. Several QTLs for flowering and maturity dates were highly stable, detected each year of evaluation, suggesting that they were not affected by climatic variations. For flowering date, major QTLs were detected on linkage groups (LG) 4 for apricot and sweet cherry and on LG6 for peach. QTLs were identified on LG2, LG3, LG4 and LG7 for the three species. For maturity date, a major QTL was detected on LG4 in the three species. Using the peach genome sequence data, candidate genes underlying the major QTLs on LG4 and LG6 were investigated and key genes were identified. Our results provide a basis for the identification of genes involved in flowering and maturity dates that could be used to develop cultivar ideotypes adapted to future climatic conditions.

Outlook for ash in your forest: results of emerald ash borer research and implications for management

Science.gov (United States)

Kathleen S. Knight

2014-01-01

Since its accidental introduction near Detroit, Michigan, in the mid-1990s, emerald ash borer (EAB) has rapidly spread through much of the U.S. and adjacent Canada, leaving millions of dead ash trees in Midwestern states (4,11). Unfortunately, EAB attacks trees as small as an inch in stem diameter and it attacks all five ash species native to the region - white, green...

Analysis of Peach Bottom station blackout with MELCOR

International Nuclear Information System (INIS)

Dingman, S.E.; Cole, R.K.; Haskin, F.E.; Summers, R.M.; Webb, S.W.

1987-01-01

A demonstration analysis of station blackout at Peach Bottom has been performed using MELCOR and the results have been compared with those from MARCON 2.1B and the Source Term Code Package (STCP). MELCOR predicts greater in-vessel hydrogen production, earlier melting and core collapse, but later debris discharge than MARCON 2.1B. The drywell fails at vessel breach in MELCOR, but failure is delayed about an hour in MARCON 2.1B. These differences are mainly due to the MELCOR models for candling during melting, in-core axial conduction, and continued oxidation and heat transfer from core debris following lower head dryout. Three sensitivity calculations have been performed with MELCOR to address uncertainties regarding modeling of the core-concrete interactions. The timing of events and the gas and radionuclide release rates are somewhat different in the base case and the three sensitivity cases, but the final conditions and total releases are similar

Trade-Off Between Fitness Gain and Cost Determines Profitability of a Peach Aphid Parasitoid.

Science.gov (United States)

Khatri, Diwas; He, Xiong Z; Wang, Qiao

2016-08-01

Aphidius colemani (Viereck) (Hymenoptera: Aphidiidae) is commercially produced and utilized for biological control of peach aphid Myzus persicae (Sulzer) (Hemiptera: Aphididae) on greenhouse crops in many countries. To provide knowledge for the evaluation of parasitoid-host interactions and development of effective mass rearing programs, we investigated how and why host age or size affected fitness gain in A. colemani We show that the parasitoid was significantly more likely to encounter larger hosts and that an encounter almost always triggered an attack attempt. However, the attack attempt did not proportionally translate into oviposition because larger aphids had greater ability to defend themselves and the parasitoid spent more time in handling larger aphids. The host age at parasitization had no effect on emergence rates and sex ratio of parasitoid progeny, suggesting that pupae and larvae have similar survival rate in hosts of different ages and/or the parasitoid females do not adjust sex allocation based on host size. When parasitizing mid-aged hosts, the parasitoid gained maximum fitness for their progeny in developmental period, body size, and parasitism. Taking all findings together, we suggest that parasitizing mid-aged green peach aphid nymphs is most profitable for A. colemani. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Freezing as a treatment to prevent the spread of coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae) in coffee

Science.gov (United States)

Coffee berry borer, Hypothenemus hampei (Ferrari) is the most serious insect pest of coffee around the world. While it is already present in most of the world’s major coffee growing regions, it is important to delay further spread and to prevent re-introductions which might include hyperparasites or...

The influence of satellite populations of emerald ash borer on projected economic damage in U.S. communities, 2010-2020

Science.gov (United States)

Kent F. Kovacs; Rodrigo J. Mercader; Robert G. Haight; Nathan W. Siegert; Deborah G. McCullough; Andrew M. Liebhold

2011-01-01

The invasion spread of the emerald ash borer (Agrilus planipennis Fairmaire) (Coleoptera: Buprestidae) is characterized by the formation of satellite populations that expand and coalesce with the continuously invading population front. As of January 2010, satellite infestations have been detected in 13 states and two Canadian provinces. Understanding...

Monitoring the establishment and flight phenology of parasitoids of emerald ash borer (Coleoptera: Buprestidae) in Michigan by using sentinel eggs and larvae

Science.gov (United States)

Kristopher J. Abell; Leah S. Bauer; Deborah L. Miller; Jian J. Duan; Roy G. Van Driesche

2016-01-01

The emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is an important invasive pest of ash (Fraxinus) trees in North America. Two larval parasitoid species, Tetrastichus planipennisi Yang (Hymenoptera: Eulophidae) and Spathius agrili Yang (Hymenoptera:...

Asian corn borer (ACB) and non-ACB pests in GM corn (Zea mays L.) in the Philippines.

Science.gov (United States)

Afidchao, Miladis M; Musters, C J M; de Snoo, Geert R

2013-07-01

The Asian corn borer (ACB), Ostrinia furnacalis (Guenée), has become the most damaging pest in corn in south-east Asia. Corn farmers in the Philippines have incurred great yield losses in the past decades because of ACB infestation. Bacillus thuringiensis (Bt) and Bt herbicide-tolerant (BtHT) corns have been developed to reduce borer attacks worldwide. This study assessed the extent of ACB and non-ACB pest infestations in both GM and non-GM corn in Isabela Province, the Philippines. Specific aims were to reinvestigate the efficacy of Bt corn in controlling ACB, to evaluate what parts of Bt corn plants are susceptible to ACB, to monitor the potential development of ACB resistance and to evaluate whether secondary pests dominate in an ACB-free Bt corn environment. The study involved preparatory interviews with farmers, site selection, field scouting and visual inspection of 200 plants along 200 m transect lines through 198 cornfields. Bt corn can efficiently reduce the ACB pest problem and reduce borer damage by 44%, to damage levels in Bt and BtHT corn of 6.8 and 7% respectively. The leaves of Bt corn were more susceptible, while cobs of Bt corn were less affected by ACB. Non-ACB pests were common in Bt toxin-free cornfields and reduced in non-GM cornfields where ACB was abundant. No secondary pest outbreaks were found in ACB-free Bt cornfields. Bt and BtHT corn hybrids containing the Cry1Ab protein performed well in Isabela Province. Reduced cob damage by ACB on Bt fields could mean smaller economic losses even with ACB infestation. The occurrence of ACB in Bt and BtHT cornfields, although at a moderate and insignificant level, could imply the potential development of resistance to Bt toxin. © 2012 Society of Chemical Industry.

Sanitation options for managing oak wood infested with the invasive goldspotted oak borer (Coleoptera: Buprestidae) in Southern California

Science.gov (United States)

Michael I. Jones; Tom W. Coleman; Andrew D. Graves; Mary Louise. Flint; Steven J. Seybold

2013-01-01

Movement of invasive wood-boring insects in wood products presents a threat to forest health and a management challenge for public and private land managers. The goldspotted oak borer, Agrilus auroguttatus Schaeffer (Coleoptera: Buprestidae), is a new pest in San Diego and Riverside Cos., CA, believed to have been introduced on firewood. This beetle...

Comparison of emerald ash borer preference for ash of different species, sun exposure, age, and stress treatments in relation to foliar volatiles and nutrition

Science.gov (United States)

Therese M. Poland; Deepa S. Pureswaran; Yigen Chen

2009-01-01

We investigated the host selection behavior and feeding preference of the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) on six different species of ash including Manchurian ash (F...

Multivariate analysis applied to the study of the relationship between soil and plant properties in a peach orchard

Directory of Open Access Journals (Sweden)

Viviane Santos Silva Terra

2014-06-01

Full Text Available In the State of Rio Grande do Sul, the municipality of Pelotas is responsible for 90 % of peach production due to its suitable climate and soil conditions. However, there is the need for new studies that aim at improved fruit quality and increased yield. The aim of this study was to evaluate the relationship that exists between soil physical properties and properties in the peach plant in the years 2010 and 2011 by the technique of multivariate canonical correlation. The experiment was conducted in a peach orchard located in the municipality of Morro Redondo, RS, Brazil, where an experimental grid of 101 plants was established. In a trench dug beside each one of the 101 plants, soil samples were collected to determine silt, clay, and sand contents, soil density, total porosity, macroporosity, microporosity, and volumetric water content in the 0.00-0.10 and 0.10-0.20 m layers, as well as the depth of the A horizon. In each plant and in each year, the following properties were assessed: trunk diameter, fruit size and number of fruits per plant, average weight of the fruit per plant, fruit pulp firmness, Brix content, and yield from the orchard. Exploratory analysis of the data was undertaken by descriptive statistics, and the relationships between the physical properties of the soil and of the plant were assessed by canonical correlation analysis. The results showed that the clay and microporosity variables were those that exhibited the highest coefficients of canonical cross-loading with the plant properties in the soil layers assessed, and that the variable of mean weight of the fruit per plant was that which had the highest coefficients of canonical loading within the plant group for the two years assessed.

Review of ecosystem level impacts of emerald ash borer on black ash wetlands: What does the future hold?

Science.gov (United States)

Randall K. Kolka; Anthony W. D' Amato; Joseph W. Wagenbrenner; Robert A. Slesak; Thomas G. Pypker; Melissa B. Youngquist; Alexis R. Grinde; Brian J. Palik

2018-01-01

The emerald ash borer (EAB) is rapidly spreading throughout eastern North America and devastating ecosystems where ash is a component tree. This rapid and sustained loss of ash trees has already resulted in ecological impacts on both terrestrial and aquatic ecosystems and is projected to be even more severe as EAB invades black ash-dominated wetlands of the western...

Water table response to harvesting and simulated emerald ash borer mortality in black ash wetlands in Minnesota, USA

Science.gov (United States)

Robert A. Slesak; Christian F. Lenhart; Kenneth N. Brooks; Anthony W. D' Amato; Brian J. Palik

2014-01-01

Black ash wetlands are seriously threatened because of the invasive emerald ash borer (EAB). Wetland hydrology is likely to be modified following ash mortality, but the magnitude of hydrological impact following loss via EAB and alternative mitigation harvests is not clear. Our objective was to assess the water table response to simulated EAB and harvesting to...

Infestation by Coffee White Stem Borer, Xylotrechus quadripes, in Relation to Soil and Plant Nutrient Content and Associated Quality Aspects

NARCIS (Netherlands)

Thapa, Sushil; Lantinga, Egbert A.

2016-01-01

Infestation by coffee white stem borer, Xylotrechus quadripes Chevrolat (Coleoptera: Cerambycidae) is becoming severe in parts of Asia and Africa. In recent years, the pest has also been found in North and South America. This study in Gulmi District, Nepal, aimed to determine the severity of

Draft genome of the most devastating insect pest of coffee worldwide: the coffee berry borer, Hypothenemus hampei

KAUST Repository

Vega, Fernando E.

2015-07-31

The coffee berry borer, Hypothenemus hampei, is the most economically important insect pest of coffee worldwide. We present an analysis of the draft genome of the coffee berry borer, the third genome for a Coleopteran species. The genome size is ca. 163 Mb with 19,222 predicted protein-coding genes. Analysis was focused on genes involved in primary digestion as well as gene families involved in detoxification of plant defense molecules and insecticides, such as carboxylesterases, cytochrome P450, gluthathione S-transferases, ATP-binding cassette transporters, and a gene that confers resistance to the insecticide dieldrin. A broad range of enzymes capable of degrading complex polysaccharides were identified. We also evaluated the pathogen defense system and found homologs to antimicrobial genes reported in the Drosophila genome. Ten cases of horizontal gene transfer were identified with evidence for expression, integration into the H. hampei genome, and phylogenetic evidence that the sequences are more closely related to bacterial rather than eukaryotic genes. The draft genome analysis broadly expands our knowledge on the biology of a devastating tropical insect pest and suggests new pest management strategies.

Draft genome of the most devastating insect pest of coffee worldwide: the coffee berry borer, Hypothenemus hampei

KAUST Repository

Vega, Fernando E.; Brown, Stuart M.; Chen, Hao; Shen, Eric; Nair, Mridul B.; Ceja-Navarro, Javier A.; Brodie, Eoin L.; Infante, Francisco; Dowd, Patrick F.; Pain, Arnab

2015-01-01

The coffee berry borer, Hypothenemus hampei, is the most economically important insect pest of coffee worldwide. We present an analysis of the draft genome of the coffee berry borer, the third genome for a Coleopteran species. The genome size is ca. 163 Mb with 19,222 predicted protein-coding genes. Analysis was focused on genes involved in primary digestion as well as gene families involved in detoxification of plant defense molecules and insecticides, such as carboxylesterases, cytochrome P450, gluthathione S-transferases, ATP-binding cassette transporters, and a gene that confers resistance to the insecticide dieldrin. A broad range of enzymes capable of degrading complex polysaccharides were identified. We also evaluated the pathogen defense system and found homologs to antimicrobial genes reported in the Drosophila genome. Ten cases of horizontal gene transfer were identified with evidence for expression, integration into the H. hampei genome, and phylogenetic evidence that the sequences are more closely related to bacterial rather than eukaryotic genes. The draft genome analysis broadly expands our knowledge on the biology of a devastating tropical insect pest and suggests new pest management strategies.

Application of indoxacarb for managing shoot and fruit borer of eggplant (Solanum melongena L.) and its decontamination.

Science.gov (United States)

Saimandir, Jayakrishnan; Gopal, Madhuban

2009-03-01

Indoxacarb was applied at 75 and 150 g a.i. ha(-1) for two years to an eggplant (Solanum melongena L.) crop grown in the field plots in order to evaluate its efficacy for management of the lepidopteron pest, shoot and fruit borer. The residues of the insecticide were quantified by high-pressure liquid chromatography (HPLC). The mean initial deposits of indoxacarb on eggplant fruits were found to be 2.60-2.634 mg kg(-1) and 3.64-3.68 mg kg(-1) from the two rates of applications, respectively. They declined with time and reached to non-detectable (indoxacarb was found to be by washing with a mixture of alkali and potassium permanganate (KMnO(4)) thereby resulting in the removal of 67.5% and 59.2 % residues for 5 and 10 microg g(-1) spiking doses, respectively. Major products formed on reaction of indoxacarb with alkali were identified by electron spray ionization chromatography/mass spectrometry (ESI/MS). The per cent reduction on the weight and number basis of treated eggplant plots were compared to those observed in control plots to demonstrate the effectiveness of indoxacarb treatment on shoot and fruit borer population.

Field-cage evaluation of the parasitoid Phymastichus coffea LaSalle (Hymenoptera: Eulophidae) as a natural enemy of the coffee berry borer

Science.gov (United States)

Phymastichus coffea (Hymenoptera: Eulophidae) is an African parasitoid that has been imported to Mexico and other Latin American countries for the biological control of the coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae). As a part of the evaluation of this ...

Sugarcane giant borer transcriptome analysis and identification of genes related to digestion.

Science.gov (United States)

Fonseca, Fernando Campos de Assis; Firmino, Alexandre Augusto Pereira; de Macedo, Leonardo Lima Pepino; Coelho, Roberta Ramos; de Souza Júnior, José Dijair Antonino; de Sousa Júnior, José Dijair Antonino; Silva-Junior, Orzenil Bonfim; Togawa, Roberto Coiti; Pappas, Georgios Joannis; de Góis, Luiz Avelar Brandão; da Silva, Maria Cristina Mattar; Grossi-de-Sá, Maria Fátima

2015-01-01

Sugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the GenBank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a number of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST database for the species. Transcriptome analysis will certainly be useful to identify novel developmental genes, to better understand the insect's biology and to guide the development of new strategies for insect-pest control.

Sugarcane giant borer transcriptome analysis and identification of genes related to digestion.

Directory of Open Access Journals (Sweden)

Fernando Campos de Assis Fonseca

Full Text Available Sugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus, a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the GenBank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB transcriptome, a number of aminopeptidase N (APN cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST database for the species. Transcriptome analysis will certainly be useful to identify novel developmental genes, to better understand the insect's biology and to guide the development of new strategies for insect-pest control.