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Sample records for papaya chilena vasconcellea

  1. Papaya Lethal Yellowing Virus (PLYV) Infects Vasconcellea cauliflora

    NARCIS (Netherlands)

    Amaral, P.P.R.; Resende, de R.O.; Souza, M.T.

    2006-01-01

    Papaya lethal yellowing virus (PLYV) é um dos três vírus descritos infectando mamoeiros (Carica papaya L.) no Brasil. Vasconcellea cauliflora (Jacq.) A. DC., antes denominada de Carica cauliflora (Jacq.), é uma reconhecida fonte de resistência natural ao Papaya ringspot virus (PRSV), causador da "Ma

  2. Distribution, diversity and environmental adaptation of highland papaya (Vasconcellea spp.) in tropical and subtropical America

    NARCIS (Netherlands)

    Scheldeman, X.; Willemen, L.; Coppens D'eeckenbrugge, G.; Romeijn-Peeters, E.; Restrepo, M.T.; Romero Motoche, J.; Jimenez, D.; Lobo, M.; Medina, C.I.; Reyes, C.; Rodriguez, D.; Ocampo, J.A.; Damme, van P.; Goetghebeur, P.

    2007-01-01

    Vasconcellea species, often referred to as highland papayas, consist of a group of fruit species that are closely related to the common papaya (Carica papaya). The genus deserves special attention as a number of species show potential as raw material in the tropical fruit industry, fresh or in proce

  3. Development of plants resistant to Papaya leaf distortion mosaic virus by intergeneric hybridization between Carica papaya and Vasconcellea cundinamarcensis.

    Science.gov (United States)

    Tarora, Kazuhiko; Shudo, Ayano; Kawano, Shinji; Yasuda, Keiji; Ueno, Hiroki; Matsumura, Hideo; Urasaki, Naoya

    2016-12-01

    In this study, we confirmed that Vasconcellea cundinamarcensis resists Papaya leaf distortion mosaic virus (PLDMV), and used it to produce intergeneric hybrids with Carica papaya. From the cross between C. papaya and V. cundinamarcensis, we obtained 147 seeds with embryos. Though C. papaya is a monoembryonic plant, multiple embryos were observed in all 147 seeds. We produced 218 plants from 28 seeds by means of embryo-rescue culture. All plants had pubescence on their petioles and stems characteristic of V. cundinamarcensis. Flow cytometry and PCR of 28 plants confirmed they were intergeneric hybrids. To evaluate virus resistance, mechanical inoculation of PLDMV was carried out. The test showed that 41 of 134 intergeneric hybrid plants showed no symptoms and were resistant. The remaining 93 hybrids showed necrotic lesions on the younger leaves than the inoculated leaves. In most of the 93 hybrids, the necrotic lesions enclosed the virus and prevented further spread. These results suggest that the intergeneric hybrids will be valuable material for PLDMV-resistant papaya breeding.

  4. Deshidratación osmótica de la papaya chilena (Vasconcellea pubescens e influencia de la temperatura y concentración de la solución sobre la cinética de transferencia de materia Desidratação osmótica do mamão chileno (Vasconcellea pubescens e influência da temperatura e concentração da solução sobre a cinética de transferência de matéria

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    Antonio Vega-Gálvez

    2007-09-01

    Full Text Available El objetivo de este trabajo fue estudiar la cinética de deshidratación osmótica de la papaya chilena (Vasconcellea pubescens, utilizando dos variables experimentales: la temperatura (30, 40, 50 °C y concentración (40, 50 y 60% de la solución osmótica. Se determinó la variación de masa total, masa de agua y masa de sólidos solubles y se modeló con las ecuaciones difusionales, además en esta investigación se consideró y utilizó una modificación de la ecuación propuesta por Biswal-Bozorgmehr. Estos modelos fueron evaluados por medio del coeficiente de regresión lineal, suma de errores cuadrados, raíz media de los errores cuadrados y Chi-cuadrado. Al comparar los valores experimentales con los calculados, se demostró que el modelo Biswal-Bozorgmehr modificado obtuvo mejor ajuste sobre la variación de masa de agua y sólidos solubles. Mediante la ecuación de Arrhenius se analizó el efecto de la temperatura sobre los parámetros cinéticos (Kw y Kss y difusividad efectiva de agua (Dwe y sólidos solubles (Dsse. De acuerdo al diseño factorial, se observó que la temperatura no influyó sobre la difusividad efectiva de agua y de sólidos, pero sí la concentración de la solución osmótica. La mejor condición de salida de agua y ganancia de sólidos solubles durante la deshidratación osmótica de la papaya chilena fue a 30 °C con una concentración del 60%.O objetivo deste trabalho foi estudar a cinética de desidratação osmótica do mamão chileno (Vasconcellea pubescens utilizando duas variáveis experimentais: temperatura (30, 40 e 50 °C e concentração (40, 50 e 60% da solução osmótica. Para este estudo, determinou-se a variação de massa total, de massa de água, de massa de sólidos solúveis, modeladas com as equações difusionais. Nesta investigação, também se considera e se usa uma modificação da equação proposta por Biswal-Bozorgmehr. Estes modelos foram avaliados através do coeficiente de regress

  5. Extraction Techniques for Bioactive Compounds and Antioxidant Capacity Determination of Chilean Papaya (Vasconcellea pubescens Fruit

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    Elsa Uribe

    2015-01-01

    Full Text Available The aim of this work was to assess and compare different extraction methods by using high hydrostatic pressure (HHPE, ultrasound (UE, agitation (AE, and their combinations for the extraction of bioactive compounds of Chilean papaya. Extract antioxidant capacity was evaluated by three methods (i.e., DPPH, FRAP, and Voltammetry and phenolic compounds and vitamin C were determined by HPLC. Papaya sample extraction was performed by HHPE at 500 MPa for 10 min and UE and AE for 30 min, respectively. The combined-extractions: HHPE-UE and HHPE-AE, were carried out for 5 min and 15 min, respectively. The highest values found were total phenolic 129.1 mg GAE/100 g FW, antioxidant capacity by DPPH 20.6 mM TE/100 g FW, and voltammetry 141.0 mM TE/100 g FW for HHPE-UE method in free compound extraction. Regarding vitamin C content, its highest value was found by HHPE-UE (74 mg/100 g FW a combined extraction method. The phenolic compounds rutin and p-coumaric acid were found in all the extracts, both in free and bound forms, respectively. Besides, the combined techniques improved the extraction of bioactive compounds.

  6. Germinación, microinjertación y cultivo de callos in vitro de Vasconcellea stipulata V.M. Badillo y Vasconcellea pubescens A.DC

    OpenAIRE

    2016-01-01

    En varias poblaciones rurales de regiones andinas y centroamericanas se utilizan con fines etnomédicos las hojas, el látex y el fruto de las plantas de la familia Caricaceae cuyo miembro más representativo es la papaya (Carica papaya L.), la cual produce la fuente comercial más importante de la enzima proteolítica papaína. El presente trabajo estudia las especies Vasconcellea stipulata V.M. Badillo y Vasconcellea pubescens A.DC que pertenecen al género Vasconcellea, miembros de la familia Car...

  7. Isolation and Characterisation of PRSV-P Resistance Genes in Carica and Vasconcellea

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    M. R. Razean Haireen

    2014-01-01

    Full Text Available Papaya (Carica papaya L. is one of the major tropical fruit crops worldwide, but it is limited throughout its range by papaya ringspot virus type P (PRSV-P. Previous genetic studies identified a functional PRSV-P resistance marker in a mapping population of F2 plants of Vasconcellea pubescens (resistant to PRSV-P × Vasconcellea parviflora (susceptible to PRSV-P and showed that the marker exhibited homology to a serine threonine protein kinase (STK gene. Full length cDNAs of putative PRSV-P resistance genes designated CP_STK from C. papaya and VP_STK1 and VP_STK2 from V. pubescens were cloned by rapid amplification of cDNA ends (RACE. Due to a frame-shift mutation, the two homologous sequences are transcribed and edited differently such that the gene product in V. pubescens is two separate transcripts, whereas in C. papaya they are fused into a single message. A peroxisomal targeting signal (PTS2 present in VP_STK2 but absent in the other transcripts may be the functional source of PRSV resistance in V. pubescens. The STK gene from V. pubescens may have been derived from an alternative splicing to confer resistance. The putative resistance gene, VP_STK2, that was identified in this study is a potential new source of PRSV-P resistance for papaya genotypes.

  8. Palynology of Carica and Vasconcellea (Caricaceae Palinología de Carica y Vasconcellea (Caricaceae

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    Lagos Túlio César

    2006-09-01

    Full Text Available

    Palynology of Carica and Vasconcellea (Caricaceae. The pollen of C. papaya and agreements of Vasconcellea cauliflora, V. cundinamarcensis, V. crassipetala, V. goudotiana, V. x heilbornii var. chrysopetala, V. longiflora and V. sphaerocarpa collected in the Colombian Coffee Growing Zone, using the technique of acetolisis for optic microscopy and the fixation procedure with glutaraldehide, dehydration and ionization with gold-palade, for scanning electronic microscopy was described. The pollen grains were characterized using descriptors, which include the characters of taxonomic value for pollen identification, genetically determined. The most important are the number, position and character of the aperture (NPC and exine ornamentation and stratification. The pollen is of medium size for both genera, tricolporate, zonoaperturate, prolate-spheroid to subprolate, isopolar radial symmetry, tectate, dug, foveolate, with columelas. These characters have demonstrated a great contribution to the taxonomy of Caricaceae because the cluster analysis allowed distinguish very well the two genera.

    Se describe el polen de C. papaya y accesiones de Vasconcellea cauliflora, V. cundinamarcensis, V. crassipetala, V. goudotiana, V. x heilbornii var. chrysopetala, V. longiflora y V. sphaerocarpa recolectadas en la Zona Cafetera de Colombia, empleando la técnica de acetólisis para microscopía óptica y el procedimiento de fijación con glutaraldehído, deshidratación e ionización con oro paladio, para microscopía electrónica de barrido (MEB. Se caracterizaron los granos de polen por medio de una lista de descriptores que incluyeron los caracteres de valor taxonómico para identi

  9. Citogenética de especies de Vasconcellea (Caricaceae

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    Creucí Maria Caetano

    2008-12-01

    Full Text Available Conocer aspectos básicos de la citogenética de las papayas de montaña o papayuelas, especies del género Vasconcellea (Caricaceae originarias de Ecuador y Colombia, fue el objetivo del trabajo. Botones florales se fijaron en etanol-acético 3:1 por 24h, luego se transfirieron a nueva solución y se almacenaron en frío. Los microsporocitos y los granos de polen se tiñieron con acetocarmín (1%. Se evaluaron las fases de meiosis y la viabilidad de polen para describir el comportamiento cromosómico. Se encontró que: 1 todas las seis especies estudiadas fueron diploides (2n=2x=18; 2 se describió el número cromosómico para V. sphaerocarpa, V. longiflora y V. palandensis (2n=18; 3 el grado de asimetría de los complementos cromosómicos indicó un proceso de evolución; 4 la presencia de numerosos NOR en V. sphaerocarpa está relacionada con los micronucleolos y corroboró el posible origen híbrido; y 5 V. cauliflora (42.98% y V. cundinamarcensis (47.93% presentaron la más baja viabilidad polínica.

  10. Intestinal obstruction due to Vasconcellea seeds: Report of three cases and literature review

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    Montoya-González, Juliana

    2015-07-01

    Full Text Available Vasconcellea spp., is a species of Andean papaya commonly cultivated in rural communities close to Medellín, Colombia. Due to the pleasant and sweet flavor of its fruits, children frequently ingest its seeds accidentally. After ingestion, the seeds are engaged in the colonic lumen and block the exit of stools, causing pain and bloating, and promoting bacterial translocation. Diagnosis is based on clinical history and rectal examination. Treatment depends on the degree of local and systemic involvement and includes disimpaction of rectal contents under general anesthesia and colonic washes with 0.9% saline solution. In severe cases derivative colostomy has been required. In the literature there are no reports of intestinal obstruction due to Vasconcellea seeds, possibly because it has been mistaken for seeds of the genus Carica. In this article, three cases treated at pediatric services in Medellín, Colombia, in 2012 and 2013 are described. We note that this is a rarely suspected disease, leading to late diagnosis and potential catastrophic consequences. It is important to educate people to prevent the ingestion of the seeds.

  11. Citogenética de especies de Vasconcellea (Caricaceae

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    Caetano Creucí María

    2008-12-01

    Full Text Available Conocer aspectos básicos de la citogenética de las papayas de montaña o papayuelas, especies del género Vasconcellea (Caricaceae originarias de Ecuador y Colombia, fue el objetivo del trabajo. Botones florales se fijaron en etanol-acético 3:1 por 24h, luego se transfirieron a nueva solución y se almacenaron en frío. Los microsporocitos y los granos de polen se tiñieron con acetocarmín (1%. Se evaluaron las fases de meiosis y la viabilidad de polen para describir el comportamiento cromosómico. Se encontró que: 1 todas las seis especies estudiadas fueron diploides (2n=2x=18; 2 se describió el número cromosómico para V. sphaerocarpa, V. longiflora y V. palandensis (2n=18; 3 el grado de asimetría de los complementos cromosómicos indicó un proceso de evolución; 4 la presencia de numerosos NOR en V. sphaerocarpa está relacionada con los micronucleolos y corroboró el posible origen híbrido; y 5 V. cauliflora (42.98% y V. cundinamarcensis (47.93% presentaron la más baja viabilidad polínica.

  12. Mejoramiento de la germinación, control de la hiperhidricidad y formación de brotes en Vasconcellea stipulata Badillo

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    Rosa Armijos González

    2015-11-01

    Full Text Available Vasconcellea stipulata has great commercial importance because of its enzymatic activity and as a source for genetic improvement of papaya since it is resistant to the papaya ringspot virus. However, due to its low regeneration by seeds and limited knowledge of its genetic and pharmaceutical properties, this species is not widely cultivated. For propagation, in vitro culture of seeds has been used to address this problem, but hyperhydricity, a physiological disorder, mainly expressed in the developing embryonic axis and specifically associated with this species, is a significant constraint. In order to obtain elite material for culture of V. stipulata, the aim of this work was to increase germination, to control hyperhydricity in embryos and to evaluate the potential to induce morphogenic responses, i.e., shoot formation. Our results showed that it is possible to increase germination up to 53% under in vitro conditions within a short period in the presence of hydrogen peroxide. In addition, hyperhydricity was significantly reduced (50% in vitro when gibberellic acid concentrations were included on a 1/2 Nitsch and Nitsch nutrient medium, resulting in approximately 80% recovery of viable seedlings. Finally, other plant growth regulators were evaluated and found to trigger shoot formation in axillary buds as well as induce the formation of callus in leaf sections derived of seedlings.

  13. A dated phylogeny of the papaya family (Caricaceae) reveals the crop's closest relatives and the family's biogeographic history.

    Science.gov (United States)

    Carvalho, Fernanda Antunes; Renner, Susanne S

    2012-10-01

    Papaya (Carica papaya) is a crop of great economic importance, and the species was among the first plants to have its genome sequenced. However, there has never been a complete species-level phylogeny for the Caricaceae, and the crop's closest relatives are therefore unknown. We investigated the evolution of the Caricaceae based on sequences from all species and genera, the monospecific Carica, African Cylicomorpha with two species, South American Jacaratia and Vasconcellea with together c. 28 species, and Mexican/Guatemalan Jarilla and Horovitzia with four species. Most Caricaceae are trees or shrubs; the species of Jarilla, however, are herbaceous. We generated a matrix of 4711 nuclear and plastid DNA characters and used maximum likelihood (ML) and Bayesian analysis to infer species relationships, rooting trees on the Moringaceae. Divergence times were estimated under relaxed and strict molecular clocks, using different subsets of the data. Ancestral area reconstruction relied on a ML approach. The deepest split in the Caricaceae occurred during the Late Eocene, when the ancestor of the Neotropical clade arrived from Africa. In South America, major diversification events coincide with the Miocene northern Andean uplift and the initial phase of the tectonic collision between South America and Panama resulting in the Panamanian land bridge. Carica papaya is sister to Jarilla/Horovitzia, and all three diverged from South American Caricaceae in the Oligocene, 27 (22-33) Ma ago, coincident with the early stages of the formation of the Panamanian Isthmus. The discovery that C. papaya is closest to a clade of herbaceous or thin-stemmed species has implications for plant breeders who have so far tried to cross papaya only with woody highland papayas (Vasconcellea).

  14. en una comuna chilena

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    Mirtha Lischetti

    2006-01-01

    Full Text Available Los procesos globalizadores encuentran en el Estado chileno un campo propicio para desarrollarse después de que la brutal represión de la dictadura militar eliminara la posibilidad de un gobierno socialista llegado al poder por vía electoral. Los contenidos ideológicos de la globalización, así como las políticas económicas concretas que se llevan a la práctica en su nombre, transforman las subjetividades de los sujetos. La clase trabajadora chilena de ideología y tradición mayoritariamente de izquierda queda marginada de la vida política dentro de su propia realidad. Nuestro análisis indaga acerca de los alcances y límites de la supresión de la vida política dentro de dicha clase y la posible construcción de una contrahegemonía que ésta pueda realizar.

  15. IN VITRO RHIZOGENESIS IN PAPAYA (CARICA PAPAYA L.)

    OpenAIRE

    Jaime A. TEIXEIRA DA SILVA

    2013-01-01

    The seeds of two papaya (Carica papaya L.) cultivars ('Rainbow' and 'Sunrise Solo') were germinated on Murashige and Skoog (MS) medium with 3% sucrose, and free of plant growth regulators. Papaya contains some important secondary metabolites such as papain, and there would be interest in the in vitro mass production of papaya tissue of uniform origin. The most obvious form would be through the induction of somatic embryos, but rhizogenesis, an unexplored method, could provide as-yet unknown a...

  16. Post-irradiation identification of papaya ( Carica papaya L.) fruit

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    Chatterjee, Suchandra; Variyar, Prasad S.; Sharma, Arun

    2012-03-01

    Impact of radiation processing on the volatile essential oil profile of papaya ( Carica papaya) was investigated. Gamma-radiation processing resulted in the appearance of a new peak in the GLC profile that was identified as phenol. The observed dose dependent increase in phenol content suggested possible use of this compound as a marker for radiation processed papaya.

  17. Anther culture of papaya (Carica papaya L.).

    Science.gov (United States)

    Tsay, H S; Su, C Y

    1985-02-01

    Papaya (Carica papaya L.) anther containing microspores in tetrad to early-binucleate stages were successfully cultured on 1/2 strength MS salts and vitamins with full strength Na-Fe-EDTA supplemented with 2 mg/l NAA, 1 mg/l BA and 6% sucrose for callus initiation and formation. Highest frequencies of callus induction were obtained when anthers at the uninucleate stage were cultured in the dark. Haploid plantlets and pollen-derived embryoids were obtained from anthers cultured at the uninucleate stage on solidified MS medium containing 3% sucrose without any growth regulators under a low light intensity (1,500 lux). Large quantities of embryoids were obtained when the original embryoids were transferred to MS medium with 3% sucrose and no growth regulators. Cytology of root tips of embryoid-derived plants confirmed the haploid chromosome number of 9 indicating that the embryoids originated from pollen.

  18. Babaco (Vasconcellea heilbornii var. pentagona Badillo. Major plant pathogens and control strategies

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    Angel Rolando Robles-Carrión

    2016-04-01

    Full Text Available This work was carried out with the aim of documenting the importance of Babaco for the Andean region, its main plant pathogens, and possible control strategies and integrated management, emphasizing the MVB. Was performed a systematic review of scientific papers published in prestigious journals and thesis diplomas, masters and doctorates from various universities. As a criterion for inclusion-exclusion studies were taken into account in the genus Vasconcellea. Ecuador has 15 of the 21 described species, of which more than half are located in the province of Loja. Vascular Wilt Babaco (MVB is caused by a complex of Fusarium fungi that interact with the plant. There are no integrated management strategies for Vascular Wilt Babaco scientifically proven.

  19. Características Físico-Químicas del Látex de Papayuelo (Vasconcellea cundinamarcensis Badillo, Caricaceae Physico-Chemical Characteristics of Papayuelo Latex (Vasconcellea Cundinamarcensis Badillo, Caricaceae

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    Leslie V Vidal

    2009-01-01

    Full Text Available Se determinó el rendimiento y la actividad enzimática de la papaína presente en el látex de papayuelo (Vasconcellea cundinamarcensis, cultivado en Cobquecura, VIII región del Bío-Bío, Chile, durante un año. Entre enero y diciembre, las plantas, frutos y látex, obtenidos de frutos inmaduros, fueron caracterizados con el propósito de conocer el comportamiento del cultivo a través de las estaciones del año, y comprender las posibles relaciones entre los parámetros estudiados. El diseño experimental fue completamente aleatorio y los datos se procesaron mediante un ANDEVA y prueba de rangos múltiples de Duncan. Se encontró diferencia estadística significativa entre estaciones en cuanto al desarrollo de la planta y sus características. Los mayores rendimientos de látex se obtuvieron en la estación de primavera, mientras que la actividad enzimática se mantuvo constante durante el año de estudio.A study to determine the yield and enzymatic activity of papain present in the latex obtained from papayuelo fruit (Vasconcellea cundinamarcensis cultivated in Cobquecura, VIII Bío-Bío Region in Chile, during one year was done. Between January and December the papayuelo plants, fruits and latex obtained from unripe fruits were characterized. The objective of the study was to know the species behavior through the seasons and the relation between the parameters studied. The experimental design was completely random, and the data were processed through ANOVA and Duncan’s multiple range test. A significant statistical difference in plant growth and plan characteristics between seasons was found. The higher latex yields were obtained in spring, whereas the enzymatic activity was constant through the year.

  20. IN VITRO RHIZOGENESIS IN PAPAYA (CARICA PAPAYA L.

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    Jaime A. TEIXEIRA DA SILVA

    2013-12-01

    Full Text Available The seeds of two papaya (Carica papaya L. cultivars ('Rainbow' and 'Sunrise Solo' were germinated on Murashige and Skoog (MS medium with 3% sucrose, and free of plant growth regulators. Papaya contains some important secondary metabolites such as papain, and there would be interest in the in vitro mass production of papaya tissue of uniform origin. The most obvious form would be through the induction of somatic embryos, but rhizogenesis, an unexplored method, could provide as-yet unknown advantages. In this study, with the objective of artificaially inducing rhizogenesis in vitro, young leaves of both cultivars were placed on MS basal medium exposed to 5 concentrations (0, 1, 2, 4 or 8 mg/l of auxins (2,4,5-trichlorophenoxyacetic acid, 2,4,5-T; indole-3-acetic acid, IAA; indole-3-butyric acid, IBA; α-naphthaleneacetic acid, NAA; β-naphthoxyacetic acid, BNOA or phloroglucinol. All auxins could induce adventitious roots. Most roots (23/explant formed with 2 mg/l NAA. The ability to induce only roots without any other intermediary organs such as callus or shoots provides an exclusive system for possible root-specific secondary metabolite production without the need for transgenic technologies such as Agrobacterium rhizogenes, or could provide a model protocol for more in-depth developmental studies on root development in papaya, an unexplored topic for this tropical plant.

  1. Las franjas electorales en la experiencia chilena

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    Juan Ignacio García Rodríguez

    2013-07-01

    Full Text Available Expone la experiencia chilena sobre el establecimiento y regulación de las franjas electorales, para lo cual analiza la constitucionalidad de la franja, los principios involucrados, las disposiciones regulatorias, el papel del Consejo Nacional de Televisión, la jurisprudencia que sobre el tema ha sido emitida por el Tribunal Calificador de Elecciones y los retos que el sistema de franjas electorales enfrenta a futuro como lo es su aplicación a las elecciones primarias de los partidos políticos.

  2. La guerra en la dramaturgia chilena

    OpenAIRE

    Faúndez Carreño, Tania

    2014-01-01

    La guerra se ha constituido un tema constante en la dramaturgia chilena, la cual a su vez se ha vinculado estrechamente con la identidad nacional. La pulsión de la guerra, con sus mecanismos discursivos institucionales (histórico, político, cultural, militar, nacional), sus ondulaciones y su dificultad misma de la representación sobre la escena han resonado en la dramaturgia patria desde la consolidación de la República en el siglo XIX (1817) hasta la apoteósica celebración del Bicentenario n...

  3. Managing papaya ringspot virus: Impact of grass barriers on alate aphid immigration into papaya orchards

    Science.gov (United States)

    Papaya ringspot virus, transmitted by alate aphids, is the most limiting factor of papaya production in the Caribbean region. Although there are transgenic papaya varieties that provide protection from this virus, these varieties are effective only in certain regions against certain strains of the v...

  4. Protection and coexistence of conventional papaya productions with PRSV resistant transgenic papaya

    Science.gov (United States)

    Papaya ringspot virus (PRSV) is a devastating disease that has a detrimental impact on both commercial papaya production and Caricaceae germplasm conservation. Transgenic line 55-1 and derived progeny ‘SunUp’ and ‘Rainbow’ are resistant to PRSV and have saved the papaya industry in Hawaii. In small...

  5. Physical-chemical analyses of irradiated papayas (Carica papaya L.)

    Energy Technology Data Exchange (ETDEWEB)

    Camargo, R.J. [Radiation Technology Center, IPEN-CNEN/SP, Av. Lineu Prestes, 2242, 05508-900, Sao Paulo, SP (Brazil); Tadini, C.C. [Chemical Engineering Department, Escola Politecnica, Sao Paulo University, P.O. Box 61548, 05424-970, SP (Brazil); Sabato, S.F. [Radiation Technology Center, IPEN-CNEN/SP, Av. Lineu Prestes, 2242, 05508-900, Sao Paulo, SP (Brazil)], E-mail: sfsabato@ipen.br

    2007-11-15

    Papaya is cultivated in Espirito Santo State/Brazil and as it stands up to irradiation, it is important to validate this technology, since it is already being applied in some countries. Penetration energy, ratio (relation between soluble solids and titrable acidity) and skin color were evaluated to verify the influence of four different doses of irradiation (0.0, 0.5, 0.75 and 1.00 kGy) on papayas, during 21 days. As a result for the skin color and the penetration energy, it was found that in the first days after irradiation, these variables increased with increase in radiation dose; however, after a time lapse, the tendency inverted and the irradiated fruits had a slower ripening process. For the ratio, a very important variable that it is responsible for the fruit taste, no difference was found between irradiated and the control fruit. Color and texture measurements are dependent on the storage temperature.

  6. Physical-chemical analyses of irradiated papayas ( Carica papaya L.)

    Science.gov (United States)

    Camargo, R. J.; Tadini, C. C.; Sabato, S. F.

    2007-11-01

    Papaya is cultivated in Espírito Santo State/Brazil and as it stands up to irradiation, it is important to validate this technology, since it is already being applied in some countries. Penetration energy, ratio (relation between soluble solids and titrable acidity) and skin color were evaluated to verify the influence of four different doses of irradiation (0.0, 0.5, 0.75 and 1.00 kGy) on papayas, during 21 days. As a result for the skin color and the penetration energy, it was found that in the first days after irradiation, these variables increased with increase in radiation dose; however, after a time lapse, the tendency inverted and the irradiated fruits had a slower ripening process. For the ratio, a very important variable that it is responsible for the fruit taste, no difference was found between irradiated and the control fruit. Color and texture measurements are dependent on the storage temperature.

  7. Ambient temperature perception in papaya for papaya ringspot virus interaction.

    Science.gov (United States)

    Mangrauthia, Satendra K; Singh Shakya, Viplendra P; Jain, R K; Praveen, Shelly

    2009-06-01

    Temperature dramatically affects the host-virus interaction. Outbreaks of viral diseases are frequently associated with the ambient temperature required for host development. Using papaya as a host and Papaya ringspot virus (PRSV) as a pathogen, we studied the effect of temperature on the intensity of disease symptoms and virus accumulation. The phenotypic expression of symptoms and viral accumulation were found to be maximum at ambient temperature (26-31 degrees C) of papaya cultivation. However, there was a drastic difference, 10 degrees C above and below the ambient temperature. The underlying mechanism of these well-known observations are not yet understood completely; however, these observations might help find answers in RNA silencing mechanism of plants. Since viral-derived silencing suppressor proteins play a significant role in RNA silencing mechanism, here we show that PRSV-derived Helper component proteinase (HC-Pro) protein has an affinity for small RNAs in a temperature-dependent manner. This suggested the probable role of HC-Pro in the temperature-regulated host-virus relationship.

  8. Potential threat of a new pathotype of Papaya leaf distortion mosaic virus infecting transgenic papaya resistant to Papaya ringspot virus.

    Science.gov (United States)

    Bau, H-J; Kung, Y-J; Raja, J A J; Chan, S-J; Chen, K-C; Chen, Y-K; Wu, H-W; Yeh, S-D

    2008-07-01

    A virus identified as a new pathotype of Papaya leaf distortion mosaic virus (PLDMV, P-TW-WF) was isolated from diseased papaya in an isolated test-field in central Taiwan, where transgenic papaya lines resistant to Papaya ringspot virus (PRSV) were evaluated. The infected plants displayed severe mosaic, distortion and shoe-stringing on leaves; stunting in apex; and water-soaking on petioles and stems. This virus, which did not react in enzyme-linked immunosorbent assay with the antiserum to the PRSV coat protein, infected only papaya, but not the other 18 plant species tested. Virions studied under electron microscope exhibited morphology and dimensions of potyvirus particles. Reverse transcription-polymerase chain reaction conducted using potyvirus-specific primers generated a 1,927-nucleotide product corresponding to the 3' region of a potyvirus, showing high sequence identity to the CP gene and 3' noncoding region of PLDMV. Search for similar isolates with the antiserum against CP of P-TW-WF revealed scattered occurrence of PLDMV in Taiwan. Phylogenetic analysis of PLDMV isolates of Taiwan and Japan indicated that the Taiwan isolates belong to a separate genetic cluster. Since all the Taiwan isolates infected only papaya, unlike the cucurbit-infecting Japanese P type isolates, the Taiwan isolates are considered a new pathotype of PLDMV. Susceptibility of all our PRSV-resistant transgenic papaya lines to PLDMV indicates that the virus is an emerging threat for the application of PRSV-resistant transgenic papaya in Taiwan and elsewhere.

  9. Anticancer activity of Carica papaya: a review.

    Science.gov (United States)

    Nguyen, Thao T T; Shaw, Paul N; Parat, Marie-Odile; Hewavitharana, Amitha K

    2013-01-01

    Carica papaya is widely cultivated in tropical and subtropical countries and is used as food as well as traditional medicine to treat a range of diseases. Increasing anecdotal reports of its effects in cancer treatment and prevention, with many successful cases, have warranted that these pharmacological properties be scientifically validated. A bibliographic search was conducted using the key words "papaya", "anticancer", and "antitumor" along with cross-referencing. No clinical or animal cancer studies were identified and only seven in vitro cell-culture-based studies were reported; these indicate that C. papaya extracts may alter the growth of several types of cancer cell lines. However, many studies focused on specific compounds in papaya and reported bioactivity including anticancer effects. This review summarizes the results of extract-based or specific compound-based investigations and emphasizes the aspects that warrant future research to explore the bioactives in C. papaya for their anticancer activities.

  10. Characterization of chromoplasts and carotenoids of red- and yellow-fleshed papaya (Carica papaya L.).

    Science.gov (United States)

    Schweiggert, Ralf M; Steingass, Christof B; Heller, Annerose; Esquivel, Patricia; Carle, Reinhold

    2011-11-01

    Chromoplast morphology and ultrastructure of red- and yellow-fleshed papaya (Carica papaya L.) were investigated by light and transmission electron microscopy. Carotenoid analyses by LC-MS revealed striking similarity of nutritionally relevant carotenoid profiles in both the red and yellow varieties. However, while yellow fruits contained only trace amounts of lycopene, the latter was found to be predominant in red papaya (51% of total carotenoids). Comparison of the pigment-loaded chromoplast ultrastructures disclosed tubular plastids to be abundant in yellow papaya, whereas larger crystalloid substructures characterized most frequent red papaya chromoplasts. Exclusively existent in red papaya, such crystalloid structures were associated with lycopene accumulation. Non-globular carotenoid deposition was derived from simple solubility calculations based on carotenoid and lipid contents of the differently colored fruit pulps. Since the physical state of carotenoid deposition may be decisive regarding their bioavailability, chromoplasts from lycopene-rich tomato fruit (Lycopersicon esculentum L.) were also assessed and compared to red papaya. Besides interesting analogies, various distinctions were ascertained resulting in the prediction of enhanced lycopene bioavailability from red papaya. In addition, the developmental pathway of red papaya chromoplasts was investigated during fruit ripening and carotenogenesis. In the early maturation stage of white-fleshed papaya, undifferentiated proplastids and globular plastids were predominant, corresponding to incipient carotenoid biosynthesis. Since intermediate plastids, e.g., amyloplasts or chloroplasts, were absent, chromoplasts are likely to emerge directly from proplastids.

  11. La izquierda chilena contemparánea

    Directory of Open Access Journals (Sweden)

    Manuel Antonio Garretón

    2006-01-01

    Full Text Available En este artículo, el autor examina las particularidades de la izquierda chilena para entender su papel en los gobiernos democráticos desde los años 70. El autor parte de la premisa de la existencia, durante los años 90, de dos izquierdas: una, el socialismo dentro de la coalición; la otra, el comunismo fuera de ella. En el trabajo se sostiene que la izquierda socialista ha sido el principal motor de la coalición y su papel específico se ha concentrado en los temas socio-económicos ligados a la igualdad, a la justicia, a los derechos humanos y a las propuestas culturales de tipo progresista. Asimismo, se analizan las particularidades esenciales de los gobiernos democráticos chilenos y se examinan los temas y las tareas pendientes. El artículo concluye con un balance del papel de la izquierda y de los retos que aún tiene por delante.

  12. Short Communication: In vitro response of papaya (Carica papaya to plant growth regulators

    Directory of Open Access Journals (Sweden)

    JAIME A. TEIXEIRA DA SILVA

    2016-01-01

    Full Text Available Abstract. Teixeira da Silva JA. 2016. In vitro response of papaya (Carica papaya to multiple plant growth regulators. Nusantara Bioscience 8: 77-82. The use of plant growth regulators (PGRs in papaya (Carica papaya L. tissue culture is essential for tissue and organ culture in vitro. In this study, in a bid to expand the information available on the response to PGRs, a wide range of PGRs, roughly divided into four groups (auxins, cytokinins, alternative PGRs, growth inhibitors and retardants was tested. Among them, the auxins 2,4-D, dicamba and picloram formed most callus (hard and soft. Callus inductions by chitosan and coconut water are novel results for papaya. Shoots only formed in response to BA and TDZ, but TDZ-induced shoots were fasciated and/or hyperhydric. These results provide novel perspectives for papaya researchers who may have recalcitrant genotypes or tissues that are unresponsive in vitro.

  13. Ocorrência de Ovulariopsis papayae Bijl em folhas de mamoeiro Occurrence of Ovulariopsis papayae Bijl in papaya leaves

    Directory of Open Access Journals (Sweden)

    Ivan José Antunes Ribeiro

    1988-01-01

    Full Text Available É citada pela primeira vez, em nossas condições, a ocorrência de oídio causado por Ovulariopsis papayae Bijl em folhas de mamoeiro (Cacica papaya L.. São descritas a sintomatologia, as características morfológicas do patógeno e o teste de patogenicidade.The occurrence causing powdery mildew in papaya leaves was observed for the first time in the State of São Paulo, Brazil. The symptomatology and the morphological characteristics of the pathogen were described and the pathogenicity was comproved.

  14. Characterization symptomatology and molecular from papaya ringspot (PRSV) that infects Carica papaya L. in northern Peru

    OpenAIRE

    Valderrama, Shirley; Universidad Nacional de Trujillo; Cedano, Carolina; Tenorio, Jorge; Romero, Javier; Carbajal, Segundo

    2016-01-01

    The objective of present study was symptomatic and molecular characterization of the virus that infects Carica papaya L. in areas of northern Peru. To do this, of different fields were collected leaves of C. papaya with mosaic symptoms, chlorosis and distortion the leaf. Sap of these leaves was inoculated mechanically onto virus-free plants of C. papaya, Chenopodium murale, Ch. amaranticolor, Ch. quinoa, Cucumis melo, C. sativus and Cucurbita pepo; which they were kept at room temperature for...

  15. Generation of transgenic papaya with double resistance to Papaya ringspot virus and Papaya leaf-distortion mosaic virus.

    Science.gov (United States)

    Kung, Yi-Jung; Bau, Huey-Jiunn; Wu, Yi-Ling; Huang, Chiung-Huei; Chen, Tsui-Miao; Yeh, Shyi-Dong

    2009-11-01

    During the field tests of coat protein (CP)-transgenic papaya lines resistant to Papaya ringspot virus (PRSV), another Potyvirus sp., Papaya leaf-distortion mosaic virus (PLDMV), appeared as an emerging threat to the transgenic papaya. In this investigation, an untranslatable chimeric construct containing the truncated CP coding region of the PLDMV P-TW-WF isolate and the truncated CP coding region with the complete 3' untranslated region of PRSV YK isolate was transferred into papaya (Carica papaya cv. Thailand) via Agrobacterium-mediated transformation to generate transgenic plants with resistance to PLDMV and PRSV. Seventy-five transgenic lines were obtained and challenged with PRSV YK or PLDMV P-TW-WF by mechanical inoculation under greenhouse conditions. Thirty-eight transgenic lines showing no symptoms 1 month after inoculation were regarded as highly resistant lines. Southern and Northern analyses revealed that four weakly resistant lines have one or two inserts of the construct and accumulate detectable amounts of transgene transcript, whereas nine resistant lines contain two or three inserts without significant accumulation of transgene transcript. The results indicated that double virus resistance in transgenic lines resulted from double or more copies of the insert through the mechanism of RNA-mediated posttranscriptional gene silencing. Furthermore, three of nine resistant lines showed high levels of resistance to heterologous PRSV strains originating from Hawaii, Thailand, and Mexico. Our transgenic lines have great potential for controlling a number of PRSV strains and PLDMV in Taiwan and elsewhere.

  16. Improving Tenderness of Spent Layer Hens Meat Using Papaya Leaves (Carica papaya

    Directory of Open Access Journals (Sweden)

    H. O. Abdalla, N. N. A. Ali, F. S. Siddig and S. A. M. Ali*

    2013-01-01

    Full Text Available Two experiments were performed to study the use of papaya leaves as a meat tenderizer. The first experiment was to evaluate the effect of papaya dry leaves added to hen’s diet before slaughter. Spent hens (n=48 were used, half of them were fed a concentrate ration containing10% dried papaya leaves powder (DPLP while others received layer ration (Control, for 10 days. The second experiment involved a comparison between papaya leaves juice (PLJ, fresh papaya leaves (FPL and vinegar solution (VS as marinades applied to meat for one or two hours before cooking. Spent layer hens (n=42 were used for tenderness evaluation method. After slaughtering and preparing the chickens two methods of cooking were used (oven and moist cooking. The cooked parts (breast, thigh and drumstick were subjected to a panel test evaluation according to a designed questionnaire. Addition of dried papaya leaves powder to spent layer hens ration significantly (P≤0.05 increased the level of meat tenderness. Moist cooking had significantly (P≤0.05 improved meat tenderization compared to oven cooking. Meat treated with fresh papaya leaves had significantly (P≤0.05 improved tenderness. It was concluded that wrapping the tough meat of spent layer hens with fresh papaya leaves for one hour and moist cooking improve tenderness of meat.

  17. Toxicity assessment of transgenic papaya ringspot virus of 823-2210 line papaya fruits.

    Science.gov (United States)

    Lin, Hsin-Tang; Yen, Gow-Chin; Huang, Ting-Tzu; Chan, Lit-Fu; Cheng, Ying-Huey; Wu, Jhaol-Huei; Yeh, Shyi-Dong; Wang, Sheng-Yang; Liao, Jiunn-Wang

    2013-02-20

    The transgenic papaya is a valuable strategy for creating plants resistant to papaya ringspot virus (PRSV) infection and increasing production. This study was further performed to evaluate the comparative toxicity effects of the newly developed transgenic line of the fruits of two backcross transgenic papaya lines (2210 and 823) and one hybrid line (823-2210) and compare to their parent non-transgenic (TN-2) counterparts. The stability analysis of coat protein (CP) of PRSV was investigated using the digestion stability assays in simulated gastric fluid (SGF), simulated intestinal fluid (SIF), and bile salts to detect the CP fragments. Results revealed that the CP fragments were rapidly hydrolyzed in SGF and were undetectable in organs and gastrointestinal contents in rats. For the genotoxicity, three in vitro assays were conducted and exhibited that non-transgenic and backcross transgenic papaya fruits were negative. Moreover, a repeated animal feeding study was conducted by feeding 2 g/kg of body weight (bw) of non-transgenic and backcross transgenic papaya fruits for 28 days in rats. There were no biological or toxicological significances between non-transgenic and backcross transgenic papaya fruits in rats. The results demonstrated that the backcross transgenic papaya fruit can be recognized as an equivalent substitution for traditional papaya in food safety.

  18. Molecular detection of Papaya meleira virus in the latex of Carica papaya by RT-PCR

    NARCIS (Netherlands)

    Araujo, de M.M.M.; Tavares, E.T.; Silva, da F.R.; Marinho, V.L.D.; Souza, M.T.

    2007-01-01

    A RT-PCR assay was developed for early and accurate detection of Papaya meleira virus (PMeV) in the latex from infected papayas. The meleira disease is characterized by an excessive exudation of more fluidic latex from fruits, leaves and stems. This latex oxidises and gives the fruit a ¿sticky¿ text

  19. Papaya (Carica papaya) lysozyme is a member of the family 19 (Basic, class II) chitinases

    NARCIS (Netherlands)

    Subroto, T; Sufiati, S; Beintema, JJ

    1999-01-01

    The most comprehensive studies on a plant lysozyme (EC 3.2.1.17) are those on the enzyme from papaya (Carica papaya) latex, published in 1967 and 1969. However, the N-terminal amino acid sequence of five amino acid sequence of this enzyme, determined by manual Edman degradation, did not allow assign

  20. Erwinia mallotivora sp., a new pathogen of papaya (Carica papaya) in Peninsular Malaysia.

    Science.gov (United States)

    Amin, Noriha Mat; Bunawan, Hamidun; Redzuan, Rohaiza Ahmad; Jaganath, Indu Bala S

    2010-12-24

    Erwinia mallotivora was isolated from papaya infected with dieback disease showing the typical symptoms of greasy, water-soaked lesions and spots on leaves. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain belonged to the genus Erwinia and was united in a monophyletic group with E. mallotivora DSM 4565 (AJ233414). Earlier studies had indicated that the causal agent for this disease was E. papayae. However, our current studies, through Koch's postulate, have confirmed that papaya dieback disease is caused by E. mallotivora. To our knowledge, this is the first new discovery of E. mallotivora as a causal agent of papaya dieback disease in Peninsular Malaysia. Previous reports have suggested that E. mallotivora causes leaf spot in Mallotus japonicus. However, this research confirms it also to be pathogenic to Carica papaya.

  1. Erwinia mallotivora sp., a New Pathogen of Papaya (Carica papaya in Peninsular Malaysia

    Directory of Open Access Journals (Sweden)

    Noriha Mat Amin

    2010-12-01

    Full Text Available Erwinia mallotivora was isolated from papaya infected with dieback disease showing the typical symptoms of greasy, water-soaked lesions and spots on leaves. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain belonged to the genus Erwinia and was united in a monophyletic group with E. mallotivora DSM 4565 (AJ233414. Earlier studies had indicated that the causal agent for this disease was E. papayae. However, our current studies, through Koch’s postulate, have confirmed that papaya dieback disease is caused by E. mallotivora. To our knowledge, this is the first new discovery of E. mallotivora as a causal agent of papaya dieback disease in Peninsular Malaysia. Previous reports have suggested that E. mallotivora causes leaf spot in Mallotus japonicus. However, this research confirms it also to be pathogenic to Carica papaya.

  2. Diversity of Papaya ringspot virus isolates in Puerto Rico

    Science.gov (United States)

    Papaya ringspot virus (PRSV) devastates papaya production worldwide. In Puerto Rico, papaya fields can be completely infected with PRSV within a year of planting. Information about the diversity of the Puerto Rican PRSV population is relevant in order to establish a control strategy in the island. T...

  3. 78 FR 48628 - Importation of Papayas From Peru

    Science.gov (United States)

    2013-08-09

    ... Ecuador (Sec. 319.56-25). The risk management document for papayas from Peru evaluated the effectiveness... from the export program of production sites with confirmed pest problems, and the papaya orchards would... used to treat papayas imported from Central America, Brazil, Colombia, and Ecuador for fruit...

  4. Papaya

    Science.gov (United States)

    ... used for preventing and treating gastrointestinal tract disorders, intestinal parasite infections, and as a sedative and diuretic. It ... a reduced risk of HPV infection. Stomach and intestine problems. Parasite infections. Other conditions. More evidence is needed to ...

  5. Correlation between ethylene emission and skin colour changes during papaya (Carica papaya L.) fruit ripening

    Science.gov (United States)

    da Silva, M. G.; Oliveira, J. G.; Vitoria, A. P.; Corrêa, S. F.; Pereira, M. G.; Campostrini, E.; Santos, E. O.; Cavalli, A.; Vargas, H.

    2005-06-01

    The skin colour changes and ethylene emission rates were monitored during papaya (C. papaya L.) fruit ripening. Two groups of papaya (‘Formosa’ and ‘Solo’) were applied in this study. The total colour difference was used as measured parameter and the corresponding half time of its saturation was used as correlation parameter. A high correlation factor between the saturation half time and corresponding climacteric peak time was found. It was concluded that high ethylene emission rate in ‘Solo’ fruit promotes a quick change of the total colour difference.

  6. Purification and characterization of peroxidase from papaya (Carica papaya) fruit.

    Science.gov (United States)

    Pandey, Veda P; Singh, Swati; Singh, Rupinder; Dwivedi, Upendra N

    2012-05-01

    Ripening of papaya fruit was found to be characterized with a decrease in peroxidase activity and its transcript. This peroxidase was purified to homogeneity through successive steps of ammonium sulfate fractionation, ion exchange and molecular exclusion chromatography. The peroxidase was purified 30.22-folds with overall recovery of 44.37% and specific activity of 68.59. Purified peroxidase was found to be a heterotrimer of ~240 kDa, containing two subunits each of 85 and one of 70 kDa. Purified enzyme exhibited pH and temperature optima of 7.0 and 40 °C, respectively. K(m) values for substrates o-dianicidin, guaiacol and ascorbic acid were found to be 0.125, 0.8 and 5.2 mM, respectively. K(m) for H(2)O(2) was found to be 0.25 mM. Salicylic acid was found to activate peroxidase up to 50 μM concentration, beyond which it acted as inhibitor. Ca(2+) and Mg(2+) activated peroxidase while sodium azide, SDS, and Triton X-100 were found to inhibit peroxidase.

  7. Quantification of the antiplasmodial alkaloid carpaine in papaya (Carica papaya) leaves.

    Science.gov (United States)

    Julianti, Tasqiah; Oufir, Mouhssin; Hamburger, Matthias

    2014-08-01

    Daily consumption of papaya (Carica papaya) leaves as greens and an herbal infusion is common in some parts of Indonesia as a means for preventing malaria. Antiplasmodial activity of the leaf extracts and of the main alkaloid carpaine were recently confirmed. A quantitative assay for determination of carpaine in papaya leaves was developed and validated. The assay involved pressurized liquid extraction and quantification with the aid of ultrahigh-performance liquid chromatography-tandem mass spectroscopy. Extraction conditions were optimized with respect to solvent, temperature, and number of extraction cycles. The ultrahigh-performance liquid chromatography-tandem mass spectroscopy assay was validated over a range of 20-5000 ng/mL (R(2) of 0.9908). A total of 29 papaya leaf samples were analyzed, and carpaine concentration in dry leaves was found to range from 0.02 to 0.31%. No obvious dependence on geographic origin and leaf maturity was observed.

  8. Heterosis in papaya: inter and intragroup analysis

    Directory of Open Access Journals (Sweden)

    Deisy Lúcia Cardoso

    2014-09-01

    Full Text Available Papaya (Carica papaya L. is a typical crop of tropical areas, and Brazil is one of the leading world producers. In recent decades, papaya culture has expanded to different regions of the country, but the number of cultivars available is still limited. In the present study, a complete diallel cross was carried out using eight accessions of papaya from the UENF/Caliman germplasm bank. Four genotypes belong to the Formosa heterotic group and four, to the Solo group. This study aimed to evaluate the occurrence and viability of exploring heterosis in heterotic intragroup hybrids. Fifty-six hybrid progenies were generated and evaluated. Among the Formosa intragroup hybrids, two hybrid combinations (MR x J4 and MR x SK showed heterosis for all traits, as well as good average total fruit production. Among the Solo intragroup hybrids, three hybrid combinations (WM x GG, WM x SS and WM x SM stand out for fruit production and high content of soluble solids. In Formosa x Solo hybrids, all hybrid combinations with the parent JS (JS x WM, JS x GG, JS x SS and JS x SM showed high fruit quality and good average for fruit production. The heterotic profile of the hybrids tested allowed the identification of promising hybrids within Formosa and Solo heterotic groups. The analysis of the canonical variables also allowed the visualization of distinct groups of hybrids, depending on the provenance of the parents.

  9. Origin and domestication of papaya Yh chromosome

    Science.gov (United States)

    Sex in papaya is controlled by a pair of nascent sex chromosomes. Females are XX, and two slightly different Y chromosomes distinguish males (XY) and hermaphrodites (XYh). The hermaphrodite-specific region of the Yh chromosome (HSY) and its X chromosome counterpart were sequenced and analyzed previo...

  10. Papaya seed represents a rich source of biologically active isothiocyanate.

    Science.gov (United States)

    Nakamura, Yoshimasa; Yoshimoto, Motoko; Murata, Yoshiyuki; Shimoishi, Yasuaki; Asai, Yumi; Park, Eun Young; Sato, Kenji; Nakamura, Yasushi

    2007-05-30

    In the present study, papaya (Carica papaya) seed and edible pulp were carefully separated and then the contents of benzyl isothiocyanate and the corresponding glucosinolate (benzyl glucosinolate, glucotropaeolin) quantified in each part. The papaya seed with myrosinase inactivation contained >1 mmol of benzyl glucosinolate in 100 g of fresh seed. This content is equivalent to that of Karami daikon (the hottest Japanese white radish) or that of cress. The papaya seed extract also showed a very high activity of myrosinase and, without myrosinase inactivation, produced 460 micromol of benzyl isothiocyanate in 100 g of seed. In contrast, papaya pulp contained an undetectable amount of benzyl glucosinolate and showed no significant myrosinase activity. The n-hexane extract of the papaya seed homogenate was highly effective in inhibiting superoxide generation and apoptosis induction in HL-60 cells, the activities of which are comparable to those of authentic benzyl isothiocyanate.

  11. Anti-inflammatory and immunomodulatory properties of Carica papaya.

    Science.gov (United States)

    Pandey, Saurabh; Cabot, Peter J; Shaw, P Nicholas; Hewavitharana, Amitha K

    2016-07-01

    Chronic inflammation is linked with the generation and progression of various diseases such as cancer, diabetes and atherosclerosis, and anti-inflammatory drugs therefore have the potential to assist in the treatment of these conditions. Carica papaya is a tropical plant that is traditionally used in the treatment of various ailments including inflammatory conditions. A literature search was conducted by using the keywords "papaya", "anti-inflammatory and inflammation" and "immunomodulation and immune" along with cross-referencing. Both in vitro and in vivo investigation studies were included. This is a review of all studies published since 2000 on the anti-inflammatory activity of papaya extracts and their effects on various immune-inflammatory mediators. Studies on the anti-inflammatory activities of recognized phytochemicals present in papaya are also included. Although in vitro and in vivo studies have shown that papaya extracts and papaya-associated phytochemicals possess anti-inflammatory and immunomodulatory properties, clinical studies are lacking.

  12. Host susceptibility of the papaya mosaic virus in Sri Lanka.

    Science.gov (United States)

    Rajapakse, R H; Herath, H M

    1981-01-01

    75 plant species from 11 families were tested in Sri Lanka for their susceptibility to transferring the papaya mosaic virus. After inoculation with this virus, six species, Cucurbita pepo, Cucumis sativus, Nicotiana tabacum, Chenopodium amaranticolor, Gomphrena globosa and Lycopersicum esculentum, developed such symptoms, and after re-isolation from the host plant the virus again infected papaya plants. Thus these species are possible alternate hosts of papaya mosaic virus in Sri Lanka.

  13. Physical Characteristics of the Leaves and Latex of Papaya Plants Infected with the Papaya meleira Virus

    Science.gov (United States)

    Magaña-Álvarez, Anuar; Vencioneck Dutra, Jean Carlos; Carneiro, Tarcio; Pérez-Brito, Daisy; Tapia-Tussell, Raúl; Ventura, Jose Aires; Higuera-Ciapara, Inocencio; Fernandes, Patricia Machado Bueno; Fernandes, Antonio Alberto Ribeiro

    2016-01-01

    Sticky disease, which is caused by Papaya meleira virus (PMeV), is a significant papaya disease in Brazil and Mexico, where it has caused severe economic losses, and it seems to have spread to Central and South America. Studies assessing the pathogen-host interaction at the nano-histological level are needed to better understand the mechanisms that underlie natural resistance. In this study, the topography and mechanical properties of the leaf midribs and latex of healthy and PMeV-infected papaya plants were observed by atomic force microscopy and scanning electron microscopy. Healthy plants displayed a smooth surface with practically no roughness of the leaf midribs and the latex and a higher adhesion force than infected plants. PMeV promotes changes in the leaf midribs and latex, making them more fragile and susceptible to breakage. These changes, which are associated with increased water uptake and internal pressure in laticifers, causes cell disruption that leads to spontaneous exudation of the latex and facilitates the spread of PMeV to other laticifers. These results provide new insights into the papaya-PMeV interaction that could be helpful for controlling papaya sticky disease. PMID:27092495

  14. The draft genome of the transgenic tropical fruit tree papaya (Carica papaya Linnaeus).

    Science.gov (United States)

    Ming, Ray; Hou, Shaobin; Feng, Yun; Yu, Qingyi; Dionne-Laporte, Alexandre; Saw, Jimmy H; Senin, Pavel; Wang, Wei; Ly, Benjamin V; Lewis, Kanako L T; Salzberg, Steven L; Feng, Lu; Jones, Meghan R; Skelton, Rachel L; Murray, Jan E; Chen, Cuixia; Qian, Wubin; Shen, Junguo; Du, Peng; Eustice, Moriah; Tong, Eric; Tang, Haibao; Lyons, Eric; Paull, Robert E; Michael, Todd P; Wall, Kerr; Rice, Danny W; Albert, Henrik; Wang, Ming-Li; Zhu, Yun J; Schatz, Michael; Nagarajan, Niranjan; Acob, Ricelle A; Guan, Peizhu; Blas, Andrea; Wai, Ching Man; Ackerman, Christine M; Ren, Yan; Liu, Chao; Wang, Jianmei; Wang, Jianping; Na, Jong-Kuk; Shakirov, Eugene V; Haas, Brian; Thimmapuram, Jyothi; Nelson, David; Wang, Xiyin; Bowers, John E; Gschwend, Andrea R; Delcher, Arthur L; Singh, Ratnesh; Suzuki, Jon Y; Tripathi, Savarni; Neupane, Kabi; Wei, Hairong; Irikura, Beth; Paidi, Maya; Jiang, Ning; Zhang, Wenli; Presting, Gernot; Windsor, Aaron; Navajas-Pérez, Rafael; Torres, Manuel J; Feltus, F Alex; Porter, Brad; Li, Yingjun; Burroughs, A Max; Luo, Ming-Cheng; Liu, Lei; Christopher, David A; Mount, Stephen M; Moore, Paul H; Sugimura, Tak; Jiang, Jiming; Schuler, Mary A; Friedman, Vikki; Mitchell-Olds, Thomas; Shippen, Dorothy E; dePamphilis, Claude W; Palmer, Jeffrey D; Freeling, Michael; Paterson, Andrew H; Gonsalves, Dennis; Wang, Lei; Alam, Maqsudul

    2008-04-24

    Papaya, a fruit crop cultivated in tropical and subtropical regions, is known for its nutritional benefits and medicinal applications. Here we report a 3x draft genome sequence of 'SunUp' papaya, the first commercial virus-resistant transgenic fruit tree to be sequenced. The papaya genome is three times the size of the Arabidopsis genome, but contains fewer genes, including significantly fewer disease-resistance gene analogues. Comparison of the five sequenced genomes suggests a minimal angiosperm gene set of 13,311. A lack of recent genome duplication, atypical of other angiosperm genomes sequenced so far, may account for the smaller papaya gene number in most functional groups. Nonetheless, striking amplifications in gene number within particular functional groups suggest roles in the evolution of tree-like habit, deposition and remobilization of starch reserves, attraction of seed dispersal agents, and adaptation to tropical daylengths. Transgenesis at three locations is closely associated with chloroplast insertions into the nuclear genome, and with topoisomerase I recognition sites. Papaya offers numerous advantages as a system for fruit-tree functional genomics, and this draft genome sequence provides the foundation for revealing the basis of Carica's distinguishing morpho-physiological, medicinal and nutritional properties.

  15. Physical Characteristics of the Leaves and Latex of Papaya Plants Infected with the Papaya meleira Virus.

    Science.gov (United States)

    Magaña-Álvarez, Anuar; Vencioneck Dutra, Jean Carlos; Carneiro, Tarcio; Pérez-Brito, Daisy; Tapia-Tussell, Raúl; Ventura, Jose Aires; Higuera-Ciapara, Inocencio; Fernandes, Patricia Machado Bueno; Fernandes, Antonio Alberto Ribeiro

    2016-04-15

    Sticky disease, which is caused by Papaya meleira virus (PMeV), is a significant papaya disease in Brazil and Mexico, where it has caused severe economic losses, and it seems to have spread to Central and South America. Studies assessing the pathogen-host interaction at the nano-histological level are needed to better understand the mechanisms that underlie natural resistance. In this study, the topography and mechanical properties of the leaf midribs and latex of healthy and PMeV-infected papaya plants were observed by atomic force microscopy and scanning electron microscopy. Healthy plants displayed a smooth surface with practically no roughness of the leaf midribs and the latex and a higher adhesion force than infected plants. PMeV promotes changes in the leaf midribs and latex, making them more fragile and susceptible to breakage. These changes, which are associated with increased water uptake and internal pressure in laticifers, causes cell disruption that leads to spontaneous exudation of the latex and facilitates the spread of PMeV to other laticifers. These results provide new insights into the papaya-PMeV interaction that could be helpful for controlling papaya sticky disease.

  16. COMPARATIVE STUDIES ON ANTHELMINTIC POTENTIAL OF CUCURBITA MAXIMA (PUMPKIN SEEDS AND CARICA PAPAYA (PAPAYA SEEDS

    Directory of Open Access Journals (Sweden)

    Sengupta Rupa

    2013-08-01

    Full Text Available The crude extract of Carica papaya (papaya seeds (CP and Cucurbita maxima (Pumpkin seeds (CM were assayed against adult earthworms (Pheretima posthuma for the evaluation of anthelmintic activity. Various concentrations of both extracts were tested and results were expressed in terms of time for paralysis (P and time for death (D of worms. Albendazole was used as a reference standard. The result showed that in both of the extracts (i.e. CP and CM dose of 60 mg / ml possesses more wormicidal activity. The time of paralysis was 1.88 ± 0.52 minute and 1.93 ± 0.57 minute whereas the time of death was 3.45 ± 0.17 minute and 4.90 ± 0.18 minute in the case of Carica papaya and Cucurbita maxima respectively. In conclusion, the use of seeds of Carica papaya (CP and Cucurbita maxima (CM for anthelmintic activity have been confirmed and further studies are suggested to isolate the active principles responsible for the activity. Both the extracts showed significant anthelmintic activity, but the comparative study showed that out of these two, Carica papaya proves to be a better anthelmintic remedy.

  17. Physical Characteristics of the Leaves and Latex of Papaya Plants Infected with the Papaya meleira Virus

    Directory of Open Access Journals (Sweden)

    Anuar Magaña-Álvarez

    2016-04-01

    Full Text Available Sticky disease, which is caused by Papaya meleira virus (PMeV, is a significant papaya disease in Brazil and Mexico, where it has caused severe economic losses, and it seems to have spread to Central and South America. Studies assessing the pathogen-host interaction at the nano-histological level are needed to better understand the mechanisms that underlie natural resistance. In this study, the topography and mechanical properties of the leaf midribs and latex of healthy and PMeV-infected papaya plants were observed by atomic force microscopy and scanning electron microscopy. Healthy plants displayed a smooth surface with practically no roughness of the leaf midribs and the latex and a higher adhesion force than infected plants. PMeV promotes changes in the leaf midribs and latex, making them more fragile and susceptible to breakage. These changes, which are associated with increased water uptake and internal pressure in laticifers, causes cell disruption that leads to spontaneous exudation of the latex and facilitates the spread of PMeV to other laticifers. These results provide new insights into the papaya-PMeV interaction that could be helpful for controlling papaya sticky disease.

  18. Proteomic analysis of papaya (Carica papaya L.) displaying typical sticky disease symptoms.

    Science.gov (United States)

    Rodrigues, Silas P; Ventura, José A; Aguilar, Clemente; Nakayasu, Ernesto S; Almeida, Igor C; Fernandes, Patricia M B; Zingali, Russolina B

    2011-07-01

    Papaya (Carica papaya L.) hosts the only described laticifer-infecting virus (Papaya meleira virus, PMeV), which is the causal agent of papaya sticky disease. To understand the systemic effects of PMeV in papaya, we conducted a comprehensive proteomic analysis of leaf samples from healthy and diseased plants grown under field conditions. First, a reference 2-DE map was established for proteins from healthy samples. A total of 486 reproducible spots were identified, and MALDI-TOF-MS/MS data identified 275 proteins accounting for 159 distinct proteins from 231 spots that were annotated. Second, the differential expression of proteins from healthy and diseased leaves was determined through parallel experiments, using 2-DE and DIGE followed by MALDI-TOF-MS/MS and LC-IonTrap-MS/MS, respectively. Conventional 2-DE analysis revealed 75 differentially expressed proteins. Of those, 48 proteins were identified, with 26 being upregulated (U) and 22 downregulated (D). In general, metabolism-related proteins were downregulated, and stress-responsive proteins were upregulated. This expression pattern was corroborated by the results of the DIGE analysis, which identified 79 differentially expressed proteins, with 23 identified (17 U and 6 D). Calreticulin and the proteasome subunits 20S and RPT5a were shown to be upregulated during infection by both 2-DE and DIGE analyses. These data may help shed light on plant responses against stresses and viral infections.

  19. Digital transcriptome analysis of putative sex-determination genes in papaya (Carica papaya.

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    Naoya Urasaki

    Full Text Available Papaya (Carica papaya is a trioecious plant species that has male, female and hermaphrodite flowers on different plants. The primitive sex chromosomes genetically determine the sex of the papaya. Although draft sequences of the papaya genome are already available, the genes for sex determination have not been identified, likely due to the complicated structure of its sex-chromosome sequences. To identify the candidate genes for sex determination, we conducted a transcriptome analysis of flower samples from male, female and hermaphrodite plants using high-throughput SuperSAGE for digital gene expression analysis. Among the short sequence tags obtained from the transcripts, 312 unique tags were specifically mapped to the primitive sex chromosome (X or Y(h sequences. An annotation analysis revealed that retroelements are the most abundant sequences observed in the genes corresponding to these tags. The majority of tags on the sex chromosomes were located on the X chromosome, and only 30 tags were commonly mapped to both the X and Y(h chromosome, implying a loss of many genes on the Y(h chromosome. Nevertheless, candidate Y(h chromosome-specific female determination genes, including a MADS-box gene, were identified. Information on these sex chromosome-specific expressed genes will help elucidating sex determination in the papaya.

  20. Digital transcriptome analysis of putative sex-determination genes in papaya (Carica papaya).

    Science.gov (United States)

    Urasaki, Naoya; Tarora, Kazuhiko; Shudo, Ayano; Ueno, Hiroki; Tamaki, Moritoshi; Miyagi, Norimichi; Adaniya, Shinichi; Matsumura, Hideo

    2012-01-01

    Papaya (Carica papaya) is a trioecious plant species that has male, female and hermaphrodite flowers on different plants. The primitive sex chromosomes genetically determine the sex of the papaya. Although draft sequences of the papaya genome are already available, the genes for sex determination have not been identified, likely due to the complicated structure of its sex-chromosome sequences. To identify the candidate genes for sex determination, we conducted a transcriptome analysis of flower samples from male, female and hermaphrodite plants using high-throughput SuperSAGE for digital gene expression analysis. Among the short sequence tags obtained from the transcripts, 312 unique tags were specifically mapped to the primitive sex chromosome (X or Y(h)) sequences. An annotation analysis revealed that retroelements are the most abundant sequences observed in the genes corresponding to these tags. The majority of tags on the sex chromosomes were located on the X chromosome, and only 30 tags were commonly mapped to both the X and Y(h) chromosome, implying a loss of many genes on the Y(h) chromosome. Nevertheless, candidate Y(h) chromosome-specific female determination genes, including a MADS-box gene, were identified. Information on these sex chromosome-specific expressed genes will help elucidating sex determination in the papaya.

  1. Influence of ripening stages on antioxidant properties of papaya fruit (Carica papaya L.)

    Science.gov (United States)

    Addai, Zuhair Radhi; Abdullah, Aminah; Mutalib, Sahilah Abd.

    2013-11-01

    Papaya (Carica papaya L. cv Eksotika) is one of the most commonly consumed tropical fruits by humans, especially Malaysians. The objective of this study was to determine the phenolic compounds and antioxidants activity in different ripening stages of papaya fruit. The fruits were harvested at five different, stages RS1, RS2, RS3, RS4, and RS5 corresponding to 12, 14, 16, 18, and 20 weeks after anthesis, respectively. Papayas fruit at five different stage of ripening were obtained from farms at Pusat Flora Cheras, JabatanPertanian and Hulu Langat Semenyih, Selangor, Malaysia. The antioxidants activity were analyzed using the total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant Power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). The analyses were conducted in triplicate and the data were subjected to statistical analysis using SPSS. The results showed significant differences (P< 0.05) were found at different stages of ripening. The total phenol content TPC, TFC, FRAP and DPPH values increased significantly (P<0.05) with the ripening process. The results showed the important role of the ripening stage in increasing the antioxidant content of papaya fruits.

  2. ANALISIS PERMEASI AIR PADA DEHIDRASI OSMOSIS PEPAYA (Carica papaya) Water Permeation Analysis on Osmotic Dehydration of Papaya (Carica papaya)

    OpenAIRE

    Sang Kompiang Wirawan; Natalia Anasta

    2013-01-01

    Fruit preservation using low temperature drying is commonly initiated by osmotic dehydration process. The osmotic time is strongly influenced by the water permeation from the fruit to the osmotic solution. This research aimed to study the osmotic dehydration process of papaya by finding out the permeation rate of water to the osmotic solution across a semi permeable membrane. The effect of temperature and osmotic solution concentration on the permeation rate were also observed. The osmotic te...

  3. PROCESOS DE CANONIZACIÓN DE LA LITERATURA CHILENA

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    Iván Carrasco M

    2008-11-01

    Full Text Available Este trabajo estudia los procesos de canonización en la literatura chilena y sus relaciones con las identidades socioculturales, desde la generación romántica de 1852 (ó 1842 hasta la última década del siglo XX, delimitando sus autores, textos y metatextos canónicos. La hipótesis que organiza el trabajo es que la literatura chilena se inició en torno a un canon único, que ha sido reemplazado a través del tiempo por cánones plurales, paralelos y contrapuestos.This paper studies the literary canonization processes in Chilean literature andtheir relations with social and cultural identities. Its purpose is to establish the boundaries of the texts and metatexts written by Chilean canonical authors, starting with the romantic generation of 1852 (or 1842 and ending up with the works published in the last decade of 'the XX century. The hypothesis of this essay is that Chilean literature can be said to have its origins focused on a single canon, which is later replaced by a series of plural, parallel and even opposite canons.

  4. Virus Resistant Transgenic Papaya: Commercial Development and Regulatory and Environmental Issues

    Science.gov (United States)

    In Hawaii, transgenic papaya resistant to Papaya ringspot virus (PRSV) was developed starting in the 1980s and released commercially in 1998 to combat the wide spread destruction of Hawaii’s papaya industry. This review describes the proactive development of the transgenic papaya and its impact on ...

  5. 7 CFR 319.56-25 - Papayas from Central America and Brazil.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Papayas from Central America and Brazil. 319.56-25... § 319.56-25 Papayas from Central America and Brazil. The Solo type of papaya may be imported into the... section and all other applicable provisions of this subpart. (a) The papayas were grown and packed...

  6. Diffraction studies of papaya mosaic virus.

    Science.gov (United States)

    Tollin, P; Bancroft, J B; Richardson, J F; Payne, N C; Beveridge, T J

    1979-10-15

    X-ray and optical diffraction studies of the flexuous papaya mosaic virus are described. The virus is constructed so that there are 35 coat protein subunits in 4 turns of the helix. The virus contains about 1410 protein subunits and 6800 nucleotides and has a molecular weight of about 33 x 10(6). The structure of tubes assembled in vitro from coat protein both in the presence and absence of nucleic acid resembles that of the native virus.

  7. Carica papaya microRNAs are responsive to Papaya meleira virus infection.

    Science.gov (United States)

    Abreu, Paolla M V; Gaspar, Clicia G; Buss, David S; Ventura, José A; Ferreira, Paulo C G; Fernandes, Patricia M B

    2014-01-01

    MicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV) is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for this miRNAs had their expression modulated in a dependent manner. This study represents a comprehensive identification of conserved miRNAs inpapaya. The data presented here might help to complement the available molecular and genomic tools for the study of papaya. The differential expression of some miRNAs and identifying their target genes will be helpful for understanding the regulation and interaction of PMeV and papaya.

  8. Gamma radiation in papaya harvested at three stages of maturation

    Energy Technology Data Exchange (ETDEWEB)

    Pimentel, Rodrigo Meirelles de Azevedo; Walder, Julio Marcos Melges [Centro de Energia Nuclear na Agricultura, Piracicaba, SP (Brazil). Lab. de Irradiacao de Alimentos]. E-mail: jmwalder@cena.usp.br

    2004-04-01

    Papaya is a fragile, perishable fruit, highly accepted worldwide. To keep the quality of papaya from harvest to the consumers, conservation techniques are often used; among them is the application of gamma irradiation. The objective of this work was to evaluate gamma irradiation in papayas harvested at three degrees of maturation, in order to increase shelf life. Papayas were harvested in perfect quality conditions and selected by skin coloration into three distinct degrees of maturation: maturation 0, or beginning of yellow coloration; maturation 1, yellow stripes more developed, and maturation 2, one third yellow. Half of them were irradiated with 0.75 kGy, while the other half became control treatment. They were analyzed in four periods of conservation, which were 1 DAI (days after irradiation refrigerated at 11 {+-} 1 deg C), 14 DAI, 14 DAI + 3 DRT (room temperature at 24 deg C {+-} 2 deg C) and 14 DAI + 6 DRT. The papaya maturation degree at harvest did not influence the radiation effect. Irradiation maintained firmness of papaya and, therefore, delayed ripening; modified the green color of papaya to a lighter, more intense tone, which determined more homogeneity in the development of the skin's yellow color (greater values of L{sup *} and chroma). There was no effect of irradiation in papaya weight loss, occurrence of diseases, chroma of flesh color, p H and total soluble solids contents. (author)

  9. 75 FR 22207 - Importation of Papayas From Colombia and Ecuador

    Science.gov (United States)

    2010-04-28

    ... Colombia and Ecuador AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION: Final rule. SUMMARY... importation of commercial shipments of fresh papayas from Colombia and Ecuador into the continental United States. The conditions for the importation of papayas from Colombia and Ecuador include requirements...

  10. Effects of chloroformic extracts from washed and unwashed papaya seeds (Carica papaya) on the sperm concentration of dogs.

    Science.gov (United States)

    Ortega-Pacheco, A; Jiménez-Coello, M; Gutiérrez-Blanco, E; Acosta-Viana, K Y; Guzmán-Marín, E; Zavala-Sánchez, M A; Montalvo-Beltrán, N E; Pérez-Gutiérrez, M S

    2010-12-01

    Papaya seeds (Carica papaya Linn) have been found to have a significant effect on sperm characteristics in some mammals, including humans, but no studies have investigated the effects on dogs. In the present study, a significant decrease in sperm concentration was observed in a group of dogs treated with extracts from washed papaya seeds, but no decrease was observed in the group of dogs treated with non-washed seeds. An important effect of extract components from washed seeds such as fatty acids is probably involved in the reduction of sperm production because of Sertoli cell damage, as has been suggested for langur monkeys. Dilution of the active components in the non-washed papaya seeds or interference with some of the components may reduce the expected effect on spermatogenesis. This first report on the effects of a chloroformic extract of papaya seeds in dogs suggests that an increased dose is necessary to achieve azoospermia.

  11. Agronomic, economic and ecological aspects of the papaya (Carica papaya) production in Tabasco, Mexico

    NARCIS (Netherlands)

    Guzmán-Ramón, E.; Gómez-Alvarez, R.; Pat-Fernández, J.M.; Pohlan, H.A.J.; Álvarez-Rivero, J.C.; Geissen, V.

    2010-01-01

    The cultivation of papaya is important in the tropic because it provides source of income to the farmer within a short time. Statistical data were obtained from farmers located in the Chontalpa, Rios and Centro-Sierra regions; the size of the survey was 67 farmers. The study shows the results of the

  12. Ranking de universidades chilenas: un análisis multivariado

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    Firinguetti Limone, Luis

    2015-06-01

    Full Text Available In this work a ranking of Chilean universities on the basis of publicly available information is developed. This ranking takes into account the multivariate character of these institutions. Also, it is noted that the results are consistent with those of a well-known international ranking that uses a different set of data, as well as with several multivariate analyses of the data considered in this study.En este trabajo se elabora un ranking de las universidades chilenas en base a información pública disponible. Dicho ranking toma en cuenta el carácter multivariado de estas instituciones. Además, se ha comprobado que los resultados del ranking son consistentes con un conocido ranking internacional construido a partir de un conjunto diferente de datos y con varios análisis multivariados realizados de la información tratada en este estudio.

  13. Feminicidios en la frontera chilena: el caso de Alto Hospicio

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    Ainhoa Vásquez Mejías

    2016-01-01

    Full Text Available Entre 1999 y el 2001, varias adolescentes desaparecieron en el norte de Chile y, posteriormente, fueron encontradas muertas. La novela Alto Hospicio (2008, de Rodrigo Ramos Bañados, recrea estos feminicidios desde la visión del cómplice del único inculpado por estos crímenes. En el presente artículo se plantea que el escritor utiliza la noción de frontera como aparato crítico, con el fin de desestabilizar barreras espaciales, simbólicas y textuales que se agrupan en cuatro ejes: Bolivia-Chile, santas-putas, racionalidad-locura, ficción-realidad. Así, la novela cuestiona y deconstruye los límites para hablarnos de estos feminicidios desde un punto indeterminado, que funciona como espejo de la incertidumbre e irresolución de este caso en la justicia chilena.

  14. Effect of Biopreservatives on Storage Life of Papaya (Carica papaya L.

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    Fatema H. Brishti

    2013-04-01

    Full Text Available Normal 0 false false false EN-US X-NONE X-NONE In this experiment the effect on post-harvest preservation of papaya (Carica papaya L. fruit coated with either Aloe gel (AG; 100% or papaya leaf extract with Aloe gel (PLEAG; 1:1 was studied. To evaluate the role of coating on ripening behavior and quality of papaya the uncoated and coated fruits were stored and ripened at room temperature (25 °C-29 °C and 82-84% relative humidity. Physico-chemical properties were analyzed at 4 day intervals during the storage period. The incidence of disease attack was also visually observed. The overall results showed the superiority of AG and PLEAG coating in lengthening the shelf-life of papaya fruit compared to controls which showed significant decay from 6th day onward and complete decay within 12 days of storage. The AG and PLEAG coated fruits maintained their shelf life for 12 days and decayed at 16th day. The coated fruits also maintained their color, flavor and firmness up to 12 days of storage. An increase in ascorbic acid content (120.2 mg/100 g was also found in coated fruits in contrast to the control (59 mg/100 g. Only 27% disease incidence was observed in AG and 13% in PLEAG coated fruits as compared to control (100% during the storage period. The results of this study show that both AG and PLEAG coatings have excellent potential to be used on fresh produce to maintain quality and extend shelf-life.

  15. Apertura y Eficiencia Productiva: La Industria Chilena, 1975-1988 Apertura y Eficiencia Productiva: La Industria Chilena, 1975-1988

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    Enrique Román

    1993-03-01

    Full Text Available Apertura y Eficiencia Productiva: La Industria Chilena, 1975-1988 The results of Chile's profound economic liberalizations remain the subject of ongoing debate. Using the Total Factor Productivity approach (TFP; we review the industrial sector performance during the 1975-1988 period. Our analysis shows that greater competitiveness dictated by economic liberalization was not accomplished through technical change but, rather, through intensive factor use (1976-81, or extensive factor use (1984-88. After exhaustion of the "easy phase", new levels of productive efficiency necessitated technical innovation, that is, investment in equipment, training. and reorganization of productive processes. However, after the 1982-83 crisis, firms did not follow this path: previous financial stress and low wage costs determined a growth path based on extensive use of labor, thereby delaying technological modernization of the industrial sector.

  16. Development of Transgenic Papaya through Agrobacterium-Mediated Transformation

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    Md. Abul Kalam Azad

    2013-01-01

    Full Text Available Transgenic papaya plants were regenerated from hypocotyls and immature zygotic embryo after cocultivation with Agrobacterium tumefaciens LBA-4404 carrying a binary plasmid vector system containing neomycin phosphotransferase (nptII gene as the selectable marker and β-glucuronidase (GUS as the reporter gene. The explants were co-cultivated with Agrobacterium tumefaciens on regeneration medium containing 500 mg/L carbenicillin + 200 mg/L cefotaxime for one week. The cocultivated explants were transferred into the final selection medium containing 500 mg/L carbenicillin + 200 mg/L cefotaxime + 50 mg/L kanamycin for callus induction as well as plant regeneration. The callus derived from the hypocotyls of Carica papaya cv. Shahi showed the highest positive GUS activities compared to Carica papaya cv. Ranchi. The transformed callus grew vigorously and formed embryos followed by transgenic plantlets successfully. The result of this study showed that the hypocotyls of C. papaya cv. Shahi and C. papaya cv. Ranchi are better explants for genetic transformation compared to immature embryos. The transformed C. papaya cv. Shahi also showed the maximum number of plant regeneration compared to that of C. papaya cv. Ranchi.

  17. Whole genome sequence analysis of unidentified genetically modified papaya for development of a specific detection method.

    Science.gov (United States)

    Nakamura, Kosuke; Kondo, Kazunari; Akiyama, Hiroshi; Ishigaki, Takumi; Noguchi, Akio; Katsumata, Hiroshi; Takasaki, Kazuto; Futo, Satoshi; Sakata, Kozue; Fukuda, Nozomi; Mano, Junichi; Kitta, Kazumi; Tanaka, Hidenori; Akashi, Ryo; Nishimaki-Mogami, Tomoko

    2016-08-15

    Identification of transgenic sequences in an unknown genetically modified (GM) papaya (Carica papaya L.) by whole genome sequence analysis was demonstrated. Whole genome sequence data were generated for a GM-positive fresh papaya fruit commodity detected in monitoring using real-time polymerase chain reaction (PCR). The sequences obtained were mapped against an open database for papaya genome sequence. Transgenic construct- and event-specific sequences were identified as a GM papaya developed to resist infection from a Papaya ringspot virus. Based on the transgenic sequences, a specific real-time PCR detection method for GM papaya applicable to various food commodities was developed. Whole genome sequence analysis enabled identifying unknown transgenic construct- and event-specific sequences in GM papaya and development of a reliable method for detecting them in papaya food commodities.

  18. Subassembly aggregates of papaya mosaic virus protein.

    Science.gov (United States)

    Erickson, J W; Hallett, F R; Bancroft, J B

    1983-08-01

    An examination of the number of subunits in small aggregates of papaya mosaic virus (PMV) coat protein is presented based on a model system which gives results consistent with the experimental observation that the 14 S subassembly species is a double disc, composed of two rows of nine subunits each. The estimated hydration of the disc, about 0.85 g 1H20/9 protein, is unusually large and indicates a cavitated structure for the disc. Comparison with other rod-shaped viruses suggests that the flexuous nature of PMV is a consequence of sparse axial inter-subunit contacts at high radius.

  19. Plant lipases: partial purification of Carica papaya lipase.

    Science.gov (United States)

    Rivera, Ivanna; Mateos-Díaz, Juan Carlos; Sandoval, Georgina

    2012-01-01

    Lipases from plants have very interesting features for application in different fields. This chapter provides an overview on some of the most important aspects of plant lipases, such as sources, applications, physiological functions, and specificities. Lipases from laticifers and particularly Carica papaya lipase (CPL) have emerged as a versatile autoimmobilized biocatalyst. However, to get a better understanding of CPL biocatalytic properties, the isolation and purification of individual C. papaya lipolytic enzymes become necessary. In this chapter, a practical protocol for partial purification of the latex-associated lipolytic activity from C. papaya is given.

  20. Isolation and functional characterization of a lycopene beta-cyclase gene that controls fruit colour of papaya (Carica papaya L.).

    Science.gov (United States)

    Devitt, Luke C; Fanning, Kent; Dietzgen, Ralf G; Holton, Timothy A

    2010-01-01

    The colour of papaya fruit flesh is determined largely by the presence of carotenoid pigments. Red-fleshed papaya fruit contain lycopene, whilst this pigment is absent from yellow-fleshed fruit. The conversion of lycopene (red) to beta-carotene (yellow) is catalysed by lycopene beta-cyclase. This present study describes the cloning and functional characterization of two different genes encoding lycopene beta-cyclases (lcy-beta1 and lcy-beta2) from red (Tainung) and yellow (Hybrid 1B) papaya cultivars. A mutation in the lcy-beta2 gene, which inactivates enzyme activity, controls lycopene production in fruit and is responsible for the difference in carotenoid production between red and yellow-fleshed papaya fruit. The expression level of both lcy-beta1 and lcy-beta2 genes is similar and low in leaves, but lcy-beta2 expression increases markedly in ripe fruit. Isolation of the lcy-beta2 gene from papaya, that is preferentially expressed in fruit and is correlated with fruit colour, will facilitate marker-assisted breeding for fruit colour in papaya and should create possibilities for metabolic engineering of carotenoid production in papaya fruit to alter both colour and nutritional properties.

  1. Mabel Condemarín: una educadora diferencial chilena

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    Jaime Caiceo Escudero

    2015-10-01

    Full Text Available Mabel Condemarín Grimberg (3/11/1931-30/03/2004 fue una importante educadora chilena a quien, por su labor, el Estado de Chile le otorgó el Premio Nacional de Ciencias de la Educación en el 2003. Fue una educadora transgresora de su tiempo porque, a pesar de su origen humilde y estar lejana a los centros de poder, logró superarse académicamente y obtener los títulos que ella deseaba, tanto en Chile como en el extranjero. Condemarín dedicó su vida a un tema que no otorga grandes beneficios: preocuparse porque los nifios mas pobres y con trastornos de aprendizaje aprendieran a leer y escribir comprensiva y correctamente. Al mismo tiempo, estaba orgullosa de su condición de género, pero ello no era problema para trabajar tanto con hombres como con mujeres. En este artículo, se busca reconstituir la vida, la obra y el pensamiento de esta educadora chilena, cuyas obras escritas se conocen en los diversos países latinoamericanos y han tenido varias ediciones, así como traducciones al portugués para Brasil. AbstractMabel Condemarin Grimberg (1931-2004 was an important  Chilean  educator who was awarded the National Prize in Learning Sciences in 2003 by the Chilean government. She was a struggler of her time because despite her humble origins and no involvement in elite groups, she achieved all her academic goals and obtained all the titles she wished not only in Chile, but also abroad. She devoted her life to a project that does not have great benefits such as caring and working for those poorest children with learning disorders, to make them read and write comprehensively and accurately. At the same time, she was proud of being a woman, and this fact was not a problem to work with both, men and women. This paper aims to reconstruct Mabel Condemarin's life, works and thoughts, whose written works are well-known and reedited in different Latin American countries; as well as some editions which have been translated into Portuguese for

  2. Evaluation of fruit leather made from two cultivars of papaya

    Directory of Open Access Journals (Sweden)

    ZUHAIR RADHI ADDAI

    2016-03-01

    Full Text Available Two papaya cultivars were used to manufacture fruit leather. The objective of this study was to formulate papaya leather from locally grown papaya using natural ingredients like pectin, honey and citric acid. The fresh fruits were pureed and mix with natural ingredients, and dried in an oven at 60°C for 12 hours. The physicochemical properties and antioxidant activity were determined.The results showed that fruit leather made from Hongkong cultivar is significantly (P<0.05 higher in sensory parameters as well as physicochemical properties and antioxidant activity. The phenolics content and antioxidant activity increased by process of drying the fruit leather compared to fresh fruits in both papaya cultivars. Therefore, the consumer requirements for healthy and safe food products were respected.

  3. ESR detection procedure of irradiated papaya containing high water content

    Science.gov (United States)

    Kikuchi, Masahiro; Shimoyama, Yuhei; Ukai, Mitsuko; Kobayashi, Yasuhiko

    2011-05-01

    ESR signals were recorded from irradiated papaya at liquid nitrogen temperature (77 K), and freeze-dried irradiated papaya at room temperature (295 K). Two side peaks from the flesh at the liquid nitrogen temperature indicated a linear dose response for 3-14 days after the γ-irradiation. The line shapes recorded from the freeze-dried specimens were sharper than those at liquid nitrogen temperature.

  4. ESR detection procedure of irradiated papaya containing high water content

    Energy Technology Data Exchange (ETDEWEB)

    Kikuchi, Masahiro, E-mail: kikuchi.masahiro@jaea.go.j [Quantum Beam Science Directorate, Japan Atomic Energy Agency, 1233 Watanuki-machi, Takasaki, Gunma 370-1292 (Japan); Shimoyama, Yuhei [Quantum Beam Science Directorate, Japan Atomic Energy Agency, 1233 Watanuki-machi, Takasaki, Gunma 370-1292 (Japan); Ukai, Mitsuko [Hokkaido University of Education, 1-2 Hachiman-cho, Hakodate, Hokkaido 040-8567 (Japan); Kobayashi, Yasuhiko [Quantum Beam Science Directorate, Japan Atomic Energy Agency, 1233 Watanuki-machi, Takasaki, Gunma 370-1292 (Japan)

    2011-05-15

    ESR signals were recorded from irradiated papaya at liquid nitrogen temperature (77 K), and freeze-dried irradiated papaya at room temperature (295 K). Two side peaks from the flesh at the liquid nitrogen temperature indicated a linear dose response for 3-14 days after the {gamma}-irradiation. The line shapes recorded from the freeze-dried specimens were sharper than those at liquid nitrogen temperature.

  5. Genetic-molecular characterization of backcross generations for sexual conversion in papaya (Carica papaya L.).

    Science.gov (United States)

    Ramos, H C C; Pereira, M G; Pereira, T N S; Barros, G B A; Ferreguetti, G A

    2014-12-04

    The low number of improved cultivars limits the expansion of the papaya crop, particularly because of the time required for the development of new varieties using classical procedures. Molecular techniques associated with conventional procedures accelerate this process and allow targeted improvements. Thus, we used microsatellite markers to perform genetic-molecular characterization of papaya genotypes obtained from 3 backcross generations to monitor the inbreeding level and parental genome proportion in the evaluated genotypes. Based on the analysis of 20 microsatellite loci, 77 genotypes were evaluated, 25 of each generation of the backcross program as well as the parental genotypes. The markers analyzed were identified in 11 of the 12 linkage groups established for papaya, ranging from 1 to 4 per linkage group. The average values for the inbreeding coefficient were 0.88 (BC1S4), 0.47 (BC2S3), and 0.63 (BC3S2). Genomic analysis revealed average values of the recurrent parent genome of 82.7% in BC3S2, 64.4% in BC1S4, and 63.9% in BC2S3. Neither the inbreeding level nor the genomic proportions completely followed the expected average values. This demonstrates the significance of molecular analysis when examining different genotype values, given the importance of such information for selection processes in breeding programs.

  6. Multivariate analysis to determine the genetic distance among backcross papaya (Carica papaya) progenies.

    Science.gov (United States)

    Ramos, H C C; Pereira, M G; Gonçalves, L S A; Berilli, A P C G; Pinto, F O; Ribeiro, E H

    2012-05-14

    Morpho-agronomic and molecular (RAPD and ISSR markers) data were used to evaluate genetic distances between papaya backcross progenies in order to help identify agronomically superior genotypes. Thirty-two papaya progenies were evaluated based on 15 morpho-agronomic characteristics, 20 ISSR and 19 RAPD primers. Manhattan, Jaccard and Gower distances were used to estimate differences based on continuous and binary data and combined analyses, respectively. Except for production, there were significant differences in the continuous variables among the genotypes. The molecular analysis revealed 193 dominant markers (ISSR and RAPD), being 53 polymorphic loci. Among the various clusters that were generated, the one based on a combined analysis of morpho-agronomic and molecular data gave the highest cophenetic correlation (0.72) compared to individual analysis, consistently allocating the progenies into six groups. We found that the Gower algorithm was more coherent in the discrimination of the genotypes, demonstrating that a combination of molecular and agronomic data is valuable for studies of genetic dissimilarity in papaya.

  7. Complete genome sequence of an isolate of papaya leaf distortion mosaic virus from commercialized PRSV-resistant transgenic papaya in China.

    Science.gov (United States)

    Tuo, D; Shen, W; Yan, P; Li, Ch; Gao, L; Li, X; Li, H; Zhou, P

    2013-01-01

    Papaya leaf distortion mosaic virus is highly destructive to commercial papaya production. Here, the complete genome sequence was determined for an isolate of papaya leaf distortion mosaic virus, designated PLDMV-DF, infecting the commercialized papaya ringspot virus (PRSV)-resistant transgenic papaya from China. Excluding the 3'-poly (A) tail, the sequence shares high sequence identity to several PLDMV isolates from Taiwan and Japan and is phylogenetically most closely related to the isolate from Japan. Infection of PLDMV-DF in transgenic PRSV-resistant papaya may indicate emergence of this disease in genetically engineered plants. The reported sequence for this isolate may help generate bi-transgenic papaya resistant to PRSV and PLDMV.

  8. Sex determination in flowering plants: papaya as a model system.

    Science.gov (United States)

    Aryal, Rishi; Ming, Ray

    2014-03-01

    Unisexuality in flowering plants evolved from a hermaphrodite ancestor. Transition from hermaphrodite to unisexual flowers has occurred multiple times across the different lineages of the angiosperms. Sexuality in plants is regulated by genetic, epigenetic and physiological mechanisms. The most specialized mechanism of sex determination is sex chromosomes. The sex chromosomes ensure the stable segregation of sexual phenotypes by preventing the recombination of sex determining genes. Despite continuous efforts, sex determining genes of dioecious plants have not yet been cloned. Concerted efforts with various model systems are necessary to understand the complex mechanism of sex determination in plants. Papaya (Carica papaya L.) is a tropical fruit tree with three sex forms, male, hermaphrodite, and female. Sexuality in papaya is determined by an XY chromosome system that is in an early evolutionary stage. The male and hermaphrodite of papaya are controlled by two different types of Y chromosomes: Y and Y(h). Large amounts of information in the area of genetics, genomics, and epigenetics of papaya have been accumulated over the last few decades. Relatively short lifecycle, small genome size, and readily available genetic and genomic resources render papaya an excellent model system to study sex determination and sex chromosomes in flowering plants.

  9. The Extraction and Properties of Carica papaya Seed Oil

    Directory of Open Access Journals (Sweden)

    Y.M. Li

    2015-04-01

    Full Text Available The main objective of the present study was to evaluate the suitability of Ultrasound-Microwave synergistic Extraction (UMAE for the recovery of papaya seed oil as compared to Ultrasound-Assisted Extraction (UAE. The efficiency of these two methods was assessed by comparing the physicochemical properties and oxidative stability of papaya seed oil. The analytic tests were color, unsaponifiable matters, iodine value, acid value and peroxide value. The fatty acid components were analyzed by GC. Results indicated that the Ultrasound-Microwave synergistic Extraction (UMAE considerably impact on the physicochemical properties of the extracted papaya seed oil, UMAE provided papaya seed oil with obviously lighter color, lower unsaponifiable matters (2.53% and higher oxidative stability (PV, 0.98 m mol/kg than that of UAE. Analysis of fatty acid composition revealed that 13 kinds of components are identified and the total amount of fatty acids accounted for 93.13% of papaya seed oil, the predominant fatty acids in papaya seed oil were oleic (18:1, 72.60%, palmitic (16:0, 18.00%, linoleic acid (18:2, 5.80% and stearic (18:0, 3.60%.

  10. Promover la salud mental con mujeres chilenas en desventaja social

    Directory of Open Access Journals (Sweden)

    M Soledad Rivera

    2004-01-01

    Full Text Available Se descrive una parte de la experiencia del proyecto Chileno/Canadiense: “Promover la salud mental de las mujeres chilenas en desventaja social: Los Secretos de Maruja”. Se describen los objetivos, las etapas del proceso, de intervención y los resultados de las evaluaciones preliminares. Con la participación de las mujeres usuárias de los centros de salud, profesionales y autoridades de la comuna de La Pintana, se construyó, aplicó y evaluó un proyecto de intervención para apoyar a las mujeres en la toma de decisiones que favorezcan su salud mental personal, familiar y social. La intervención contempló el diseño, producción, distribución y evaluación de 12 folletos educativos tipo calendarios, cada uno respecto a tres temas de salud mental identificados por las mismas mujeres: Identidad y autoestima, Vínculo y Comunicación y Autocuidado. Además se involucró a las enfermeras que trabajan en los Centros de Salud, quienes incorporaron esta estrategia a la atención de salud habitual y evaluaron su impacto en la identidad, autonomía profesional y en la mejoría de la calidad del cuidado

  11. Cyanogenesis in glucosinolate-producing plants: Carica papaya and Carica quercifolia

    DEFF Research Database (Denmark)

    Olafsdottir, E.S.; Jørgensen, Lise Bolt; Jaroszewski, Jerzy W.

    2002-01-01

    Carica papaya, Carica quercifolia, Carica hastata, Caricaceae, Passifloraceae, Biosynthesis, Glucosinolates, Cyanohydrin glycosides, Cyanogenic glycosides, Prunasin, Tetraphyllin B, Cyclopentenylglycine......Carica papaya, Carica quercifolia, Carica hastata, Caricaceae, Passifloraceae, Biosynthesis, Glucosinolates, Cyanohydrin glycosides, Cyanogenic glycosides, Prunasin, Tetraphyllin B, Cyclopentenylglycine...

  12. Efficacy of four plant extracts on nematodes associated with papaya in Sindh, Pakistan

    Science.gov (United States)

    This investigation examines the effect of ethanol extracts of four plant species--Azadirachta indica (neem), Withania somnifera (ashwagandha), Tagetes erecta (marigold) and Eucalyptus citriodora (eucalyptus)--against nematodes associated with papaya (Carica papaya), and it assesses their influence o...

  13. COMUNEROS ANDINOS EN LA ADMINISTRACIÓN CHILENA (ARICA, 1880-1929

    Directory of Open Access Journals (Sweden)

    ALBERTO DÍAZ ARAYA

    2011-05-01

    Full Text Available Resumen Se evalúa el impacto generado en los sectores rurales andinos de Arica por la implementación de la estructura y burocracia administrativa chilena post Guerra del Pacífico. A través de registros documentales se hace explícita la participación de comuneros indígenas en la trama estatal chilena en un período de conflicto político y de identidades nacionales. Palabras claves: Comunidad andina – administración chilena – Arica y Tacna. Abstract Assesses the impact generated in the rural Andes Arica for implementing the administrative structure and bureaucracy in Chile after the Pacific War. Through documentary record makes explicit the participation of indigenous community members in the plot Chilean state over a period of political and national identities. Keywords : Andean Community - Chilean government - Arica and Tacna.

  14. Rapid sex identification of papaya (Carica papaya) using multiplex loop-mediated isothermal amplification (mLAMP).

    Science.gov (United States)

    Hsu, Te-Hua; Gwo, Jin-Chywan; Lin, Kuan-Hung

    2012-10-01

    Papaya (Carica papaya L.) is established as a cash crop throughout the tropical and subtropical regions due to its easy adaptation to diverse agricultural conditions, high yields, and prompt returns. The sex types of papaya plants are hermaphrodite, male, and female. Among them, hermaphroditic plants are the major type in papaya production, because the fruit has commercial advantages over that of the other sexes. Sex inheritance in papaya is determined by the M and M(h) dominant alleles in males and hermaphrodites, respectively, and a recessive m allele in females. Currently, all hermaphrodite seeds are not available due to the lethality of dominant homozygosity. Therefore, in this study, six male-hermaphrodite-specific markers were developed for a rapid sex identification using multiplex loop-mediated isothermal amplification (mLAMP) to efficiently and precisely select hermaphroditic individuals in the seedling or early growth stage. The LM1-LAMP assay consisted of two sex-LAMP reactions for amplifying two male-specific markers (T12 and Cpsm90) in one reaction, and showed several advantages in terms of a rapid reaction time (papaya production.

  15. Screening of papaya accessions resistant to Papaya lethal yellowing virus and capacity of Tetranychus urticae to transmit the virus

    Directory of Open Access Journals (Sweden)

    Marcos Fernando Basso

    2015-02-01

    Full Text Available The objective of this work was to produce a polyclonal antiserum against the coat protein (CP of Papaya lethal yellowing virus (PLYV and to determine its specificity and sensibility in the diagnosis of the virus, as well as to evaluate the genetic resistance to PLYV in papaya (Carica papaya accessions and to investigate the capacity of the two-spotted spider mite Tetranychus urticae to acquire and transmit PLYV to the plants. Sixty-five papaya accessions were evaluated. For each accession, ten plants were mechanically inoculated using PLYV-infected plant extracts, and three plants were mock inoculated with phosphate buffer alone and used as negative controls. Ninety days after inoculation, newly-emerging systemic leaves were collected from the inoculated plants, and viral infection was diagnosed by indirect Elisa, using polyclonal antiserum sensible to the in vitro-expressed PLYV CP. Viral transmission by T. urticae was evaluated in greenhouse. The experiments were repeated twice. Polyclonal antiserum recognized the recombinant PLYV CP specifically and discriminated PLYV infection from infections caused by other plant viruses. Out of the 65 papaya accessions evaluated, 15 were considered resistant, 18 moderately resistant, and 32 susceptible. The two-spotted spider mite T. urticae was capable of acquiring PLYV, but not of transmitting it to papaya.

  16. Nucleotide sequence of papaya mosaic virus RNA.

    Science.gov (United States)

    Sit, T L; Abouhaidar, M G; Holy, S

    1989-09-01

    The RNA genome of papaya mosaic virus is 6656 nucleotides long [excluding the poly(A) tail] with six open reading frames (ORFs) more than 200 nucleotides long. The four nearest the 5' end each overlap with adjacent ORFs and could code for proteins with Mr 176307, 26248, 11949 and 7224 (ORFs 1 to 4). The fifth ORF produces the capsid protein of Mr 23043 and the sixth ORF, located completely within ORF1, could code for a protein with Mr 14113. The translation products of ORFs 1 to 3 show strong similarity with those of other potexviruses but the ORF 4 protein has only limited similarity with the other potexvirus ORF 4 proteins of 7K to 11K.

  17. Origin and domestication of papaya Yh chromosome.

    Science.gov (United States)

    VanBuren, Robert; Zeng, Fanchang; Chen, Cuixia; Zhang, Jisen; Wai, Ching Man; Han, Jennifer; Aryal, Rishi; Gschwend, Andrea R; Wang, Jianping; Na, Jong-Kuk; Huang, Lixian; Zhang, Lingmao; Miao, Wenjing; Gou, Jiqing; Arro, Jie; Guyot, Romain; Moore, Richard C; Wang, Ming-Li; Zee, Francis; Charlesworth, Deborah; Moore, Paul H; Yu, Qingyi; Ming, Ray

    2015-04-01

    Sex in papaya is controlled by a pair of nascent sex chromosomes. Females are XX, and two slightly different Y chromosomes distinguish males (XY) and hermaphrodites (XY(h)). The hermaphrodite-specific region of the Y(h) chromosome (HSY) and its X chromosome counterpart were sequenced and analyzed previously. We now report the sequence of the entire male-specific region of the Y (MSY). We used a BAC-by-BAC approach to sequence the MSY and resequence the Y regions of 24 wild males and the Y(h) regions of 12 cultivated hermaphrodites. The MSY and HSY regions have highly similar gene content and structure, and only 0.4% sequence divergence. The MSY sequences from wild males include three distinct haplotypes, associated with the populations' geographic locations, but gene flow is detected for other genomic regions. The Y(h) sequence is highly similar to one Y haplotype (MSY3) found only in wild dioecious populations from the north Pacific region of Costa Rica. The low MSY3-Y(h) divergence supports the hypothesis that hermaphrodite papaya is a product of human domestication. We estimate that Y(h) arose only ∼ 4000 yr ago, well after crop plant domestication in Mesoamerica >6200 yr ago but coinciding with the rise of the Maya civilization. The Y(h) chromosome has lower nucleotide diversity than the Y, or the genome regions that are not fully sex-linked, consistent with a domestication bottleneck. The identification of the ancestral MSY3 haplotype will expedite investigation of the mutation leading to the domestication of the hermaphrodite Y(h) chromosome. In turn, this mutation should identify the gene that was affected by the carpel-suppressing mutation that was involved in the evolution of males.

  18. The dsRNA Virus Papaya Meleira Virus and an ssRNA Virus Are Associated with Papaya Sticky Disease.

    Directory of Open Access Journals (Sweden)

    Tathiana Ferreira Sá Antunes

    Full Text Available Papaya sticky disease, or "meleira", is one of the major diseases of papaya in Brazil and Mexico, capable of causing complete crop loss. The causal agent of sticky disease was identified as an isometric virus with a double stranded RNA (dsRNA genome, named papaya meleira virus (PMeV. In the present study, PMeV dsRNA and a second RNA band of approximately 4.5 kb, both isolated from latex of papaya plants with severe symptoms of sticky disease, were deep-sequenced. The nearly complete sequence obtained for PMeV dsRNA is 8,814 nucleotides long and contains two putative ORFs; the predicted ORF1 and ORF2 display similarity to capsid proteins and RdRp's, respectively, from mycoviruses tentatively classified in the family Totiviridae. The sequence obtained for the second RNA is 4,515 nucleotides long and contains two putative ORFs. The predicted ORFs 1 and 2 display 48% and 73% sequence identity, respectively, with the corresponding proteins of papaya virus Q, an umbravirus recently described infecting papaya in Ecuador. Viral purification in a sucrose gradient allowed separation of particles containing each RNA. Mass spectrometry analysis indicated that both PMeV and the second RNA virus (named papaya meleira virus 2, PMeV2 were encapsidated in particles formed by the protein encoded by PMeV ORF1. The presence of both PMeV and PMeV2 was confirmed in field plants showing typical symptoms of sticky disease. Interestingly, PMeV was detected alone in asymptomatic plants. Together, our results indicate that sticky disease is associated with double infection by PMeV and PMeV2.

  19. Development and Validation of a Multiplex Reverse Transcription PCR Assay for Simultaneous Detection of Three Papaya Viruses

    OpenAIRE

    Tuo, Decai; Shen, Wentao; Yang, Yong; Yan, Pu; Li, Xiaoying; Zhou, Peng

    2014-01-01

    Papaya ringspot virus (PRSV), Papaya leaf distortion mosaic virus (PLDMV), and Papaya mosaic virus (PapMV) produce similar symptoms in papaya. Each threatens commercial production of papaya on Hainan Island, China. In this study, a multiplex reverse transcription PCR assay was developed to detect simultaneously these three viruses by screening combinations of mixed primer pairs and optimizing the multiplex RT-PCR reaction conditions. A mixture of three specific primer pairs was used to amplif...

  20. Analysis of ripening-related gene expression in papaya using an Arabidopsis-based microarray

    Directory of Open Access Journals (Sweden)

    Fabi João Paulo

    2012-12-01

    Full Text Available Abstract Background Papaya (Carica papaya L. is a commercially important crop that produces climacteric fruits with a soft and sweet pulp that contain a wide range of health promoting phytochemicals. Despite its importance, little is known about transcriptional modifications during papaya fruit ripening and their control. In this study we report the analysis of ripe papaya transcriptome by using a cross-species (XSpecies microarray technique based on the phylogenetic proximity between papaya and Arabidopsis thaliana. Results Papaya transcriptome analyses resulted in the identification of 414 ripening-related genes with some having their expression validated by qPCR. The transcription profile was compared with that from ripening tomato and grape. There were many similarities between papaya and tomato especially with respect to the expression of genes encoding proteins involved in primary metabolism, regulation of transcription, biotic and abiotic stress and cell wall metabolism. XSpecies microarray data indicated that transcription factors (TFs of the MADS-box, NAC and AP2/ERF gene families were involved in the control of papaya ripening and revealed that cell wall-related gene expression in papaya had similarities to the expression profiles seen in Arabidopsis during hypocotyl development. Conclusion The cross-species array experiment identified a ripening-related set of genes in papaya allowing the comparison of transcription control between papaya and other fruit bearing taxa during the ripening process.

  1. Physico-chemical characteristics of papaya (Carica papaya L.) seed oil of the Hong Kong/Sekaki variety.

    Science.gov (United States)

    Yanty, Noorzianna Abdul Manaf; Marikkar, Jalaldeen Mohammed Nazrim; Nusantoro, Bangun Prajanto; Long, Kamariah; Ghazali, Hasanah Mohd

    2014-01-01

    A study was carried out to determine the physicochemical characteristics of the oil derived from papaya seeds of the Hong Kong/Sekaki variety. Proximate analysis showed that seeds of the Hong Kong/Sekaki variety contained considerable amount of oil (27.0%). The iodine value, saponification value, unsaponifiable matter and free fatty acid contents of freshly extracted papaya seed oil were 76.9 g I2/100g oil, 193.5 mg KOH/g oil, 1.52% and 0.91%, respectively. The oil had a Lovibond color index of 15.2Y + 5.2B. Papaya seed oil contained ten detectable fatty acids, of which 78.33% were unsaturated. Oleic (73.5%) acid was the dominant fatty acids followed by palmitic acid (15.8%). Based on the high performance liquid chromatography (HPLC) analysis, seven species of triacylglycerols (TAGs) were detected. The predominant TAGs of papaya seed oil were OOO (40.4%), POO (29.1%) and SOO (9.9%) where O, P, and S denote oleic, palmitic and stearic acids, respectively. Thermal analysis by differential scanning calorimetry (DSC) showed that papaya seed oil had its major melting and crystallization transitions at 12.4°C and -48.2°C, respectively. Analysis of the sample by Z-nose (electronic nose) instrument showed that the sample had a high level of volatile compounds.

  2. Simultaneous detection of papaya ringspot virus, papaya leaf distortion mosaic virus, and papaya mosaic virus by multiplex real-time reverse transcription PCR.

    Science.gov (United States)

    Huo, P; Shen, W T; Yan, P; Tuo, D C; Li, X Y; Zhou, P

    2015-12-01

    Both the single infection of papaya ringspot virus (PRSV), papaya leaf distortion mosaic virus (PLDMV) or papaya mosaic virus (PapMV) and double infection of PRSV and PLDMV or PapMV which cause indistinguishable symptoms, threaten the papaya industry in Hainan Island, China. In this study, a multiplex real-time reverse transcription PCR (RT-PCR) was developed to detect simultaneously the three viruses based on their distinctive melting temperatures (Tms): 81.0±0.8°C for PRSV, 84.7±0.6°C for PLDMV, and 88.7±0.4°C for PapMV. The multiplex real-time RT-PCR method was specific and sensitive in detecting the three viruses, with a detection limit of 1.0×10(1), 1.0×10(2), and 1.0×10(2) copies for PRSV, PLDMV, and PapMV, respectively. Indeed, the reaction was 100 times more sensitive than the multiplex RT-PCR for PRSV, and 10 times more sensitive than multiplex RT-PCR for PLDMV. Field application of the multiplex real-time RT-PCR demonstrated that some non-symptomatic samples were positive for PLDMV by multiplex real-time RT-PCR but negative by multiplex RT-PCR, whereas some samples were positive for both PRSV and PLDMV by multiplex real-time RT-PCR assay but only positive for PLDMV by multiplex RT-PCR. Therefore, this multiplex real-time RT-PCR assay provides a more rapid, sensitive and reliable method for simultaneous detection of PRSV, PLDMV, PapMV and their mixed infections in papaya.

  3. Effect of papaya seed extract on microenvironment of cauda epididymis

    Institute of Scientific and Technical Information of China (English)

    R.J. Verma; N.J. Chinoy

    2001-01-01

    Aim: To evaluate the effect of aqueous Carica papaya seed extract on microenvironment of cauda epididymis.Methods: Adult male albino rats were intrauscularly administered with 0 (control) or 0.5 mg papaya seed ex tract/kg body weight for 7 days. Cauda epididymal tubular content was collected by micropuncture technique; epididy real luminal fluid and sperm pellets were separately analyzed. Results: The results revealed that the extract treat ment caused significant reduction, as compared with control, in total protein and sialic acid contents in both epididymal fluid and sperm pellet. As compared with control, significantly lowered acid phosphatase activity was recorded in spermpellet but was higher in epididymal fluid after the treatment. The extract treatment also caused significant reduction in level of inorganic phosphorus in the ePididymal fluid. Conclusion: It is concluded that the aqueous papaya seed ex tract alters cauda epididymal microenvironment.

  4. Removal of hazardous pharmaceutical dyes by adsorption onto papaya seeds.

    Science.gov (United States)

    Weber, Caroline Trevisan; Collazzo, Gabriela Carvalho; Mazutti, Marcio Antonio; Foletto, Edson Luiz; Dotto, Guilherme Luiz

    2014-01-01

    Papaya (Carica papaya L.) seeds were used as adsorbent to remove toxic pharmaceutical dyes (tartrazine and amaranth) from aqueous solutions, in order to extend application range. The effects of pH, initial dye concentration, contact time and temperature were investigated. The kinetic data were evaluated by the pseudo first-order, pseudo second-order and Elovich models. The equilibrium was evaluated by the Langmuir, Freundlich and Temkin isotherm models. It was found that adsorption favored a pH of 2.5, temperature of 298 K and equilibrium was attained at 180-200 min. The adsorption kinetics followed the pseudo second-order model, and the equilibrium was well represented by the Langmuir model. The maximum adsorption capacities were 51.0 and 37.4 mg g(-1) for tartrazine and amaranth, respectively. These results revealed that papaya seeds can be used as an alternative adsorbent to remove pharmaceutical dyes from aqueous solutions.

  5. Field released transgenic papaya effect on soil microbial communities and enzyme activities

    Institute of Scientific and Technical Information of China (English)

    WEI Xiang-dong; ZOU Hui-ling; CHU Lee-min; LIAO Bin; YE Chang-min; LAN Chong-yu

    2006-01-01

    Soil properties, microbial communities and enzyme activities were studied in soil amended with replicase (RP)-transgenic or non-transgenic papaya under field conditions. Compared with non-transgenic papaya, significant differences (P<0.05) were observed in total nitrogen in soils grown with transgenic papaya. There were also significant differences (P<0.05) in the total number of colony forming units (CFUs) of bacteria, actinomycetes and fungi between soils amended with RP-transgenic plants and non-transgenic plants. Compared with non-transgenic papaya, the total CFUs of bacteria, actinomycetes and fungi in soil with transgenic papaya increased by 0.43-1.1, 0.21-0.80 and 0.46-0.73 times respectively. Significantly higher (P<0.05) CFUs of bacteria, actinomycetes and fungi resistant to kanamycin (Km) were obtained in soils with RP-transgenic papaya than those with non-transgenic papaya in all concentrations of Km. Higher resistance quotients for Kmr (kanam ycin resistant) bacteria, actinomycetes and fungi were found in soil planted with RP-transgenic papaya, and the resistance quotients for KTr bacteria, actinomycetes and fungi in soils with transgenic papaya increased 1.6-4.46, 0.63-2.5 and 0.75-2.30 times. RP-transgenic papaya and non-transgenic papaya produced significantly different enzyme activities in arylsulfatase (5.4-5.9x), polyphenol oxidase (0.7-1.4x), invertase (0.5-0.79x), cellulase (0.23-0.35x) and phosphodiesterase (0.16-0.2x). The former three soil enzymes appeared to be more sensitive to the transgenic papaya than the others, and could be useful parameters in assessing the effects of transgenic papaya.Transgenic papaya could alter soil chemical properties, enzyme activities and microbial communities.

  6. Folate levels and polyglutamylation profiles of papaya (Carica papaya cv. Maradol) during fruit development and ripening.

    Science.gov (United States)

    Ramos-Parra, Perla A; García-Salinas, Carolina; Hernández-Brenes, Carmen; de la Garza, Rocío I Díaz

    2013-04-24

    Folates are essential micronutrients for humans, and their deficiency causes several detrimental effects on human health. Papaya fruit is an important natural source of some micronutrients. This paper presents a first complete characterization of folate derivatives accumulated in cv. Maradol papaya during fruit development and ripening processes. During postharvest ripening, the fruit accumulated up to 24.5% of the daily folate recommended dietary allowance (RDA) for an adult in a 1 cup (145 g) portion. Tetrahydrofolate (THF) and 5-methyl-THF were the predominant folate classes observed. Surprisingly, an unusually long polyglutamylation profile of tentatively up to 17 glutamates linked to 5-methyl-THF was detected; to the authors' knowledge, this very long polyglutamyl tail has not been reported for any organism, and it is probably characteristic of this plant species. This polyglutamylation degree changed throughout fruit development and ripening, showing the largest differences at the onset of ripening. This work raises questions about the functional role of folate derivatives in fruit development.

  7. Purification of a thermostable alkaline laccase from papaya (Carica papaya) using affinity chromatography.

    Science.gov (United States)

    Jaiswal, Nivedita; Pandey, Veda P; Dwivedi, Upendra N

    2015-01-01

    A laccase from papaya leaves was purified to homogeneity by a two step procedure namely, heat treatment (at 70 °C) and Con-A affinity chromatography. The procedure resulted in 1386.7-fold purification of laccase with a specific activity of 41.3 units mg(-1) and an overall yield of 61.5%. The native purified laccase was found to be a hexameric protein of ∼ 260 kDa. The purified enzyme exhibited acidic and alkaline pH optima of 6.0 and 8.0 with the non-phenolic substrate (ABTS) and phenolic substrate (catechol), respectively. The purified laccase was found to be thermostable up to 70 °C such that it retained ∼ 80% activity upon 30 min incubation at 70 °C. The Arrhenius energy of activation for purified laccase was found to be 7.7 kJ mol(-1). The enzyme oxidized various phenolic and non-phenolic substrates having catalytic efficiency (K(cat)/K(m)) in the order of 7.25>0.67>0.27 mM(-1) min(-1) for ABTS, catechol and hydroquinone, respectively. The purified laccase was found to be activated by Mn(2+), Cd(2+), Ca(2+), Na(+), Fe(2+), Co(2+) and Cu(2+) while weakly inhibited by Hg(2+). The properties such as thermostability, alkaline pH optima and metal tolerance exhibited by the papaya laccase make it a promising candidate enzyme for industrial exploitation.

  8. Papaya (Carica papaya) lipase with some distinct acyl and alkyl specificities as compared with microbial lipases.

    Science.gov (United States)

    Gandhi, N N; Mukherjee, K D

    2000-12-01

    Lipase from papaya (Carica papaya) latex (CPL), Candida antarctica lipase B (Novozym 435, NOV) and Rhizomucor miehei lipase (Lipozyme IM 20, LIP) were used as biocatalysts for the esterification of caprylic acid with straight-chain saturated C(4)-C(18) alcohols and unsaturated C(18) alcohols, such as cis-9-octadecenyl (oleyl, C(18:1), n-9), cis-6-octadecenyl (petroselinyl, C(18:1), n-12), cis-9,cis-12-octadecadienyl (linoleyl, C(18:2), n-6), all-cis-9,12,15-octadecatrienyl (alpha-linolenyl, C(18:3), n-3) and all-cis-6,9,12-octadecatrienyl (gamma-linolenyl, C(18:3), n-6) alcohols. With CPL, highest activity was found in the esterification of octanol and decanol, whereas both NOV and LIP showed a broad chain-length-specificity for the alcohols. CPL, as opposed to the microbial lipases, strongly discriminated against all the saturated long-chain ( > C(12)) and unsaturated C(18) alcohols.

  9. INFLUENCE OF THICKNESS ON THE DRYING OF PAPAYA PUREE (Carica papaya L. THROUGH REFRACTANCE WINDOWTM TECHNOLOGY

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    MARÍA U. OCORÓ-ZAMORA

    2013-01-01

    Full Text Available El objetivo de este trabajo fue evaluar la influencia del espesor de las muestras (2, 3 y 4 mm sobre las cinéticas de secado, actividad de agua (aw y color (∆E de rodajas de puré de papaya procesadas por tecnología de ventana de refractanciaTM (RWTM. Adicionalmente se evaluó la difusividad del agua (Deff teniendo en cuenta el encogimiento. Los datos de humedad se ajustaron mediante los modelos de Newton y Midilli. Los resultados mostraron que a menor espesor el secado fue más rápido, los valores de aw fueron menores y los ∆E superiores. Las muestras alcanzaron 0.0652, 0.1132 y 0.2624 g agua/ g sólido seco en 60 min para rodajas de 2, 3 y 4 mm, respectivamente. El Modelo de Midilli fue el más apropiado para predecir las curvas de secado de papaya por RWTM. Deff disminuyó a menor espesor y su orden de magnitud fue de 10-10 m2/s.

  10. Imobilisasi Enzim Papain Dari Getah Papaya Dengan Alginant

    OpenAIRE

    Firman Sebayang

    2009-01-01

    Telah dilakukan isolasi enzim papain dari getah papaya (Carica papaya). Isolasi papain dalam penelitian ini menggunakan aseton 85% sebagai pengendap enzim. Setelah melalui tahap penyaringan dan pengeringan beku diperoleh papain 1,25 gram untuk setiap 50 ml getah. Imobilisasi isolat enzim papain dengan metoda penjebakan(entrapment) menggunakan alginat sebagai matrik polimer. pH optimum enzim imobil diperoleh 7,0 dan temperatur optimum 55oC dengan aktivitas enzim 55,60 unit/ml, sedangkan pH opt...

  11. A coating of chitosan and propolis extract for the postharvest treatment of papaya (Carica papaya L. cv. Hawaiiana

    Directory of Open Access Journals (Sweden)

    Elizabeth Barrera

    2015-06-01

    Full Text Available Propolis is a natural antimicrobial that can be used asa bioadditive in coatings to control fruit quality losses. The effectof two coatings was evaluated, a control (chitosan, 1% and atreatment (chitosan, 1%; containing propolisethanolic extract, 5%,on the microbiological and physicochemical properties of papayafruits. The chemical profile of the propolis revealed the presenceof fatty acids and their esters, carbohydrates, diterpenic acids,and pentacyclic triterpenes. The fruits covered with the treatmentdemonstrated a reduced deterioration index and infection diameterof the fungus Colletotrichum gloeosporioides, as compared tothe control papayas, postponing the appearance of damage bytwo days. Additionally, the treatment did not significantly affectthe physicochemical properties of the papaya, as compared tothe control. In conclusion, the coating formulated with propolisexhibited an in situ fungicidal and bactericidal effect withoutaltering the physiological changes of the papaya fruit duringstorage.

  12. Papaya fruit quality management during the postharvest supply chain

    Science.gov (United States)

    Papayas are popular in tropical and subtropical regions and are being exported in large volumes to Europe, the U.S. and Japan. The fruit has excellent taste, exotic flavor and nutritional properties, being rich in vitamins A, C, and antioxidants. However, due to its highly perishable nature it has n...

  13. An ultrasonic system for determining papaya physiological properties

    Science.gov (United States)

    Ibrahim, Sallehuddin; Ramli, Azlin; Yunus, Mohd Amri Md

    2015-05-01

    There is an increasing need for high quality fruit. As such it is important to have a fast, accurate and reliable method for measuring and monitoring the quality of fruit from the field to the consumer. This paper presents an investigation on the use of a non-destructive ultrasonic system which can be used to measure the quality of papaya.

  14. Genetic diversity of papaya ring spot virus in Puerto Rico

    Science.gov (United States)

    The Food and Agriculture Organization estimates that 20-40% of crop yield is lost due to pests and diseases. Viruses are agents that cause diseases which contribute greatly to the global yield loss. Because of this, food production is negatively affected, especially in the tropics. Carica papaya, co...

  15. Estimation of papaya leaf area using the central vein length

    Directory of Open Access Journals (Sweden)

    Campostrini Eliemar

    2001-01-01

    Full Text Available Four genotypes of papaya (Carica papaya L. two from the 'Solo' group (Sunrise Solo and Improved Sunrise Solo line 72/12 and two from the 'Formosa' group (Tainung 02 and Known-You 01, grown in Macaé, RJ, Brazil (lat. 22(0 24' S, long. 41(0 42' W, were used in this study. Twenty-five mature leaves from each genotype were sampled four and five months after seedling transplant to the field to determine the length of the leaf central vein (LLCV and the leaf area (LA. According to covariance analyses there were no significant differences in the slope and intercept of the mathematical models calculated for each genotype. Thus, a single mathematical model (Log LA = 0.315 + 1.85 Log LLCV, R²=0.898 was adjusted to estimate the LA using the length of LLCV for the four genotypes. An unique model can be applied to estimate the LA for the four papaya genotypes using LLCV in the range from 0.25 to 0.60 m, and for papaya trees 150 to 180 days after transplanting.

  16. Administration Dependent Antioxidant Effect of Carica papaya Seeds Water Extract

    Directory of Open Access Journals (Sweden)

    Elisa Panzarini

    2014-01-01

    Full Text Available Carica papaya is widely used in folk medicine as herbal remedy to prevent, protect against, and cure several diseases. These curative properties are based on the presence in different parts of the plant of phytochemical nutrients with antioxidant effect. Seeds are the less exploited part; thus this study is aimed at assessing the antioxidant activities of the C. papaya seeds water extract against hydrogen peroxide (H2O2 oxidative stress in human skin Detroit 550 fibroblasts. C. papaya seeds water extract is not toxic and acts as a potent free radical scavenger, providing protection to Detroit 550 fibroblasts that underwent H2O2 oxidative stress. Data show that (i the maximum protective effect is achieved by the simultaneous administration of the extract with 1 mM H2O2; (ii the extract in presence of an oxidative stress does not increase catalase activity and prevents the release of cytochrome C and the inner mitochondrial transmembrane potential (Δψm loss; (iii the extract is more efficient than vitamin C to hamper the oxidative damage; (iv the purified subfractions of the seeds water extract exert the same antioxidant effect of whole extract. In conclusion, C. papaya seeds water extract is potentially useful for protection against oxidative stress.

  17. Factibilidad técnica de la utilización de cobertura vegetal en papaya (Carica papaya L. mediante la aplicación localizada de herbicidas

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    Antonio Bogantes

    2004-01-01

    Full Text Available Factibilidad técnica de la utilización de cobertura vegetal en papaya (Carica papaya L. mediante la aplicación localizada de herbicidas. Se estudió la factibilidad de utilizar cobertura vegetal en papaya (Carica papaya L. bajo un sistema de siembra sobre lomillos. El objetivo fue establecer un área libre de malezas sobre el lomillo para permitir la colocación del fertilizante cerca del sistema radical de la papaya. Se evaluó el control de malezas de cuatro herbicidas (gifosato, oxifluorfen, pendimetalina y acetaclor sobre especies de hoja ancha, poáceas y ciperáceas, así como el posible daño de los herbicidas al cultivo y su efecto sobre el crecimiento y rendimiento de las plantas de papaya. El herbicida glifosato fue eficiente en el control de poáceas pero no contra hojas anchas. Se obtuvo muy buen control general de malezas hasta los 90 después de la aplicación con los tratamientos preemergentes oxifluorfen+pendimetalina y acetaclor. Los dos primeros herbicidas afectaron levemente las plantas de papaya cuando se aplicaron a los 10 días después de la siembra. El acetaclor no causó daño en las plantas de papaya en ninguna de las aplicaciones. No hubo diferencias entre los tratamientos con respecto a las variables de crecimiento y rendimiento del cultivo.

  18. Development of a gene-centered ssr atlas as a resource for papaya (Carica papaya marker-assisted selection and population genetic studies.

    Directory of Open Access Journals (Sweden)

    Newton Medeiros Vidal

    Full Text Available Carica papaya (papaya is an economically important tropical fruit. Molecular marker-assisted selection is an inexpensive and reliable tool that has been widely used to improve fruit quality traits and resistance against diseases. In the present study we report the development and validation of an atlas of papaya simple sequence repeat (SSR markers. We integrated gene predictions and functional annotations to provide a gene-centered perspective for marker-assisted selection studies. Our atlas comprises 160,318 SSRs, from which 21,231 were located in genic regions (i.e. inside exons, exon-intron junctions or introns. A total of 116,453 (72.6% of all identified repeats were successfully mapped to one of the nine papaya linkage groups. Primer pairs were designed for markers from 9,594 genes (34.5% of the papaya gene complement. Using papaya-tomato orthology assessments, we assembled a list of 300 genes (comprising 785 SSRs potentially involved in fruit ripening. We validated our atlas by screening 73 SSR markers (including 25 fruit ripening genes, achieving 100% amplification rate and uncovering 26% polymorphism rate between the parental genotypes (Sekati and JS12. The SSR atlas presented here is the first comprehensive gene-centered collection of annotated and genome positioned papaya SSRs. These features combined with thousands of high-quality primer pairs make the atlas an important resource for the papaya research community.

  19. Development of a gene-centered ssr atlas as a resource for papaya (Carica papaya) marker-assisted selection and population genetic studies.

    Science.gov (United States)

    Vidal, Newton Medeiros; Grazziotin, Ana Laura; Ramos, Helaine Christine Cancela; Pereira, Messias Gonzaga; Venancio, Thiago Motta

    2014-01-01

    Carica papaya (papaya) is an economically important tropical fruit. Molecular marker-assisted selection is an inexpensive and reliable tool that has been widely used to improve fruit quality traits and resistance against diseases. In the present study we report the development and validation of an atlas of papaya simple sequence repeat (SSR) markers. We integrated gene predictions and functional annotations to provide a gene-centered perspective for marker-assisted selection studies. Our atlas comprises 160,318 SSRs, from which 21,231 were located in genic regions (i.e. inside exons, exon-intron junctions or introns). A total of 116,453 (72.6%) of all identified repeats were successfully mapped to one of the nine papaya linkage groups. Primer pairs were designed for markers from 9,594 genes (34.5% of the papaya gene complement). Using papaya-tomato orthology assessments, we assembled a list of 300 genes (comprising 785 SSRs) potentially involved in fruit ripening. We validated our atlas by screening 73 SSR markers (including 25 fruit ripening genes), achieving 100% amplification rate and uncovering 26% polymorphism rate between the parental genotypes (Sekati and JS12). The SSR atlas presented here is the first comprehensive gene-centered collection of annotated and genome positioned papaya SSRs. These features combined with thousands of high-quality primer pairs make the atlas an important resource for the papaya research community.

  20. Callus induction in papaya (Carica papaya L. and synseed production for low temperature storage and cryopreservation

    Directory of Open Access Journals (Sweden)

    Teixeira da Silva Jaime A.

    2014-12-01

    Full Text Available The mid- to long-term preservation of papaya (Carica papaya L. would allow for the safeguarding of important germplasm. In this study, soft friable callus (SFC and hard callus (HC were induced from the first two true leaves of 10-day-old seedlings containing a midrib derived from the germinated seed of two cultivars (‘Rainbow’ and ‘Sunrise Solo’. Following germination on a Murashige and Skoog (MS medium that contained 3% sucrose and was free of plant growth regulators (PGRs, sections of the first true leaves from 10-day-old seedlings were exposed to seven published callus or somatic embryogenesis protocols for zygotic embryos, leaves or hypocotyls. Optimal SFC and HC induction was carried out on a half-strength MS medium following the Fitch (1993 or the Ascêncio-Cabral et al. (2008 protocol, respectively. SFC formed shoots that could then convert to plants when transferred to a full-strength MS medium devoid of PGRs. Plantlets 10-cm tall were acclimatised in two steps: first by in vitro acclimatisation in aerated vessels, the Vitron, under CO2-enriched (3000 ppm CO2, then by the transfer of individually rooted plantlets in Rockwool® blocks to a substrate of soil: pine bark : perlite (1:1:1, v/v/v. SFC and HC were then encapsulated in alginate beads, which were exposed to low temperature storage (LTS at 10°C and 15°C, and also cryopreserved for 30 days. All encapsulated alginate beads that contained SFC, HC or leaf tissue that had been stored under LTS or cryopreserved were able to regenerate callus when placed on an optimal callus induction medium. Plants derived from the control, LTS and cryopreservation protocols, either from SFC or HC, were successfully acclimatised.

  1. DESHIDRATACIÓN OSMÓTICA DE FRUTOS DE PAPAYA HAWAIANA (Carica papaya L. EN CUATRO AGENTES EDULCORANTES OSMOTIC DEHYDRATION OF HAWAIIAN PAPAYA FRUITS (Carica papaya L. USING FOUR SWEETENER AGENTS

    Directory of Open Access Journals (Sweden)

    Margarita Maria Ríos Pérez

    2005-12-01

    Full Text Available Trozos de papaya hawaiiana (Carica papaya L. fueron sometidos a un proceso de osmo-deshidratación usando cuatro agentes edulcorantes: miel de abejas, miel de caña, crema de miel de abejas y sacarosa en medio acuoso a 79 grados Brix, temperatura de 20 ºC y 23 horas de inmersión. Los resultados estadísticos mostraron que el agente de mayor capacidad deshidratante fue la miel de abejas y el menor la sacarosa. Además, los análisis cinéticos indicaron que la máxima transferencia de masa ocurre en las primeras cuatro horas del proceso y la máxima pérdida de masa del producto que puede ser alcanzada fue de 32 % con un contenido de humedad final en los frutos de papaya osmodeshidratada de 41,3 % b.h.Pieces of Hawaiian papaya (Carica papaya L. were subjected to osmotic dehydration using four sweetener agents: honey, molasses, honey cream and sucrose in aqueous solution to 79 degrees Brix, 20 ºC temperature and 23 hours of immersion. The statistical results showed that honey was the sweetener agent with highest osmotic capacity while sucrose had the lowest. The kinetic analysis also showed that the maximum mass transfer occurs during the first four hours of the process and the maximum mass loss of the product that can be attained was 32 % with a final moisture content of 41,3 % w.b.

  2. Antagonism or synergism between papaya ringspot virus and papaya mosaic virus in Carica papaya is determined by their order of infection.

    Science.gov (United States)

    Chávez-Calvillo, Gabriela; Contreras-Paredes, Carlos A; Mora-Macias, Javier; Noa-Carrazana, Juan C; Serrano-Rubio, Angélica A; Dinkova, Tzvetanka D; Carrillo-Tripp, Mauricio; Silva-Rosales, Laura

    2016-02-01

    Antagonism between unrelated plant viruses has not been thoroughly described. Our studies show that two unrelated viruses, papaya ringspot virus (PRSV) and papaya mosaic virus (PapMV) produce different symptomatic outcomes during mixed infection depending on the inoculation order. Synergism occurs in plants infected first with PRSV or in plants infected simultaneously with PRSV and PapMV, and antagonism occurs in plants infected first with PapMV and later inoculated with PRSV. During antagonism, elevated pathogenesis-related (PR-1) gene expression and increased reactive oxygen species production indicated the establishment of a host defense resulting in the reduction in PRSV titers. Polyribosomal fractioning showed that PRSV affects translation of cellular eEF1α, PR-1, β-tubulin, and PapMV RNAs in planta, suggesting that its infection could be related to an imbalance in the translation machinery. Our data suggest that primary PapMV infection activates a defense response against PRSV and establishes a protective relationship with the papaya host.

  3. Purification and characterization of a papaya (Carica papaya L.) pectin methylesterase isolated from a commercial papain preparation

    Science.gov (United States)

    We purified a single stable pectin methylesterase (CpL-PME; EC 3.1.1.11) from a commercial papain preparation, which is isolated from Carica papaya (L.) fruit latex. This CpL-PME was separated from the abundant cysteine endopeptidases activities using sequential hydrophobic interaction and cation-ex...

  4. Portable chlorophyll meter (PCM-502) values are related to total chlorophyll concentration and photosynthetic capacity in papaya (Carica papaya L.)

    Science.gov (United States)

    This study was carried out to verify the practical use of the portable chlorophyll meter-PCM502 (PCM) in two papaya cultivars with contrasting green coloring of the leaf blade (‘Golden’: yellowish-green; ‘Solo’: dark green). The relationship was studied between the photosynthetic process and leaf n...

  5. ANALISIS PERMEASI AIR PADA DEHIDRASI OSMOSIS PEPAYA (Carica papaya Water Permeation Analysis on Osmotic Dehydration of Papaya (Carica papaya

    Directory of Open Access Journals (Sweden)

    Sang Kompiang Wirawan

    2013-11-01

    Full Text Available Fruit preservation using low temperature drying is commonly initiated by osmotic dehydration process. The osmotic time is strongly influenced by the water permeation from the fruit to the osmotic solution. This research aimed to study the osmotic dehydration process of papaya by finding out the permeation rate of water to the osmotic solution across a semi permeable membrane. The effect of temperature and osmotic solution concentration on the permeation rate were also observed. The osmotic temperatures were 30, 40, 50, and 60oC, and the osmotic solution concentration were 55, 60, 65, and 70%. In order to study the water permeation through the semi permeable membrane, a simple mass transfer mathematical models based on Fick’s and Van’t Hoff laws were applied. Generally, higher temperature and osmotic solution concentration, higher water permeation rate was achieved. The results also showed that osmotic dehydration has the same identity or characteristic as drying process at high temperature. The water removal from product slices into the osmotic solution increased by the increase of osmotic period. The mass transfer coefficient and effective diffusivity of water increased when temperature and osmotic solution concentration were increased. However, the membrane permeability has a special characteristic at the ranges conditions studied. Keywords: Osmotic, permeation, dehydration, permeable, membrane ABSTRAK Pengawetan buah dengan pengeringan suhu rendah pada umumnya diawali dengan proses dehidrasi osmosis. Lama waktu osmosis sangat dipengaruhi oleh permeasi air dari bahan ke larutan osmosis. Penelitian ini bertujuan untuk mempelajari proses dehidrasi osmosis dan kecepatan permeasi air dari pepaya ke larutan gula sebagai larutan osmosis melalui membran semipermeabel. Pengaruh suhu dan konsentrasi larutan osmosis terhadap kecepatan permeasi air juga diamati. Suhu osmosis adalah 30, 40, 50, dan 60oC, sedangkan konsentrasi larutan osmosis 55, 60

  6. Reverse transcription loop-mediated isothermal amplification assay for rapid detection of Papaya ringspot virus.

    Science.gov (United States)

    Shen, Wentao; Tuo, Decai; Yan, Pu; Yang, Yong; Li, Xiaoying; Zhou, Peng

    2014-08-01

    Papaya ringspot virus (PRSV) and Papaya leaf distortion mosaic virus (PLDMV), which causes disease symptoms similar to PRSV, threaten commercial production of both non-transgenic-papaya and PRSV-resistant transgenic papaya in China. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to detect PLDMV was developed previously. In this study, the development of another RT-LAMP assay to distinguish among transgenic, PRSV-infected and PLDMV-infected papaya by detection of PRSV is reported. A set of four RT-LAMP primers was designed based on the highly conserved region of the P3 gene of PRSV. The RT-LAMP method was specific and sensitive in detecting PRSV, with a detection limit of 1.15×10(-6)μg of total RNA per reaction. Indeed, the reaction was 10 times more sensitive than one-step RT-PCR. Field application of the RT-LAMP assay demonstrated that samples positive for PRSV were detected only in non-transgenic papaya, whereas samples positive for PLDMV were detected only in commercialized PRSV-resistant transgenic papaya. This suggests that PRSV remains the major limiting factor for non-transgenic-papaya production, and the emergence of PLDMV threatens the commercial transgenic cultivar in China. However, this study, combined with the earlier development of an RT-LAMP assay for PLDMV, will provide a rapid, sensitive and cost-effective diagnostic power to distinguish virus infections in papaya.

  7. Effect of gamma irradiation in papaya (Carica papaya L.) harvested in three degrees of maturation.; Efeito da irradiacao gama em mamao papaia (Carica papaya L.) colhido em tres pontos de maturacao

    Energy Technology Data Exchange (ETDEWEB)

    Pimentel, Rodrigo Meirelles de Azevedo

    2001-07-01

    The papaya is a fragile tropical fruit with thin skin, susceptible to post harvest diseases and mechanical injuries. Furthermore, it is sensible to low temperatures and at normal conditions it ripens rapidly, increasing the difficulties with storage. The objective of this work was to evaluate the influence of gamma irradiation in papayas harvested in three degrees of maturation, in order to increase shelf life. To accomplish that, papayas were harvested in perfect quality conditions, washed, submitted to carnauba wax and selected by skin coloration into three distinct degrees of maturation: Mat 0, or beginning of yellow coloration; Mat 1, yellow stripes more developed, and Mat 2, one third yellow. Half of them were submitted to irradiation with 0, 75 kGy, while the others became control treatment. They were analyzed in four different periods of conservation, which were 1 day after irradiation (DAI) refrigerated (11 +- 1 deg C), 14 DAI refrigerated, 14 DAI refrigerated + 3 at room temperature (RT = 24 deg +- 2 deg C) and 14 DAI refrigerated + 6 at RT. The effect of irradiation was not influenced by the maturation degree at harvest. Irradiation promoted firmness maintenance of papaya and, therefore, delayed ripening; modified the green color of papaya to a lighter tone, which determined more homogeneity in the skin yellow color development (greater values of L* and b*), and turned the papaya flesh color lighter (rower values of b*). There was no effect of irradiation in papaya weight loss, in the occurrence of diseases, in the development of surface yellow color, in the parameter a* of papaya skin color, in the parameters L*, a* and chroma of flesh color, p H and total soluble solids content. Visual and organoleptic sensorial tests were accomplished with papayas from a new delivery in the conservation period of 14 DAI refrigerated + 3 at RT. In the visual test was evaluated the appearance of papaya in the following treatments: Mat O control, Mat O irradiated, Mat 1

  8. 76 FR 49725 - Notice of Decision To Authorize the Importation of Fresh Papaya Fruit From Malaysia into the...

    Science.gov (United States)

    2011-08-11

    ... Importation of Fresh Papaya Fruit From Malaysia into the Continental United States AGENCY: Animal and Plant... authorize the importation into the continental United States of fresh papaya fruit from Malaysia. Based on... weeds via the importation of fresh papaya fruit from Malaysia. DATES: Effective Date: August 11,...

  9. 77 FR 31294 - Notice of Request for Extension of Approval of an Information Collection; Importation of Papaya...

    Science.gov (United States)

    2012-05-25

    ... Collection; Importation of Papaya From Colombia and Ecuador AGENCY: Animal and Plant Health Inspection... with regulations for the importation of commercial shipments of fresh papaya from Colombia and Ecuador... importation of commercial shipments of fresh papaya from Colombia and Ecuador, contact Ms. Dorothy...

  10. 40 CFR 174.515 - Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Coat Protein of Papaya Ringspot Virus...-INCORPORATED PROTECTANTS Tolerances and Tolerance Exemptions § 174.515 Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance. Residues of Coat Protein of Papaya Ringspot Virus are...

  11. Development of transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus (PRSV) = Desenvolvimento de mamoeiros transgênicos resistentes a vírus expressando o gene da capa protéica de um isolado brasileiro de Papaya ringspot virus

    NARCIS (Netherlands)

    Souza, M.T.; Níckel, O.; Gonsalves, D.

    2005-01-01

    Translatable and nontranslatable versions of the coat protein (cp) gene of a Papaya ringspot virus (PRSV) isolate collected in the state of Bahia, Brazil, were engineered for expression in Sunrise and Sunset Solo varieties of papaya (Carica papaya). The biolistic system was used to transform seconda

  12. Dengue fever treatment with Carica papaya leaves extracts.

    Science.gov (United States)

    Ahmad, Nisar; Fazal, Hina; Ayaz, Muhammad; Abbasi, Bilal Haider; Mohammad, Ijaz; Fazal, Lubna

    2011-08-01

    The main objective of the current study is to investigate the potential of Carica papaya leaves extracts against Dengue fever in 45 year old patient bitten by carrier mosquitoes. For the treatment of Dengue fever the extract was prepared in water. 25 mL of aqueous extract of C. papaya leaves was administered to patient infected with Dengue fever twice daily i.e. morning and evening for five consecutive days. Before the extract administration the blood samples from patient were analyzed. Platelets count (PLT), White Blood Cells (WBC) and Neutrophils (NEUT) decreased from 176×10(3)/µL, 8.10×10(3)/µL, 84.0% to 55×10(3)/µL, 3.7×10(3)/µL and 46.0%. Subsequently, the blood samples were rechecked after the administration of leaves extract. It was observed that the PLT count increased from 55×10(3)/µL to 168×10(3)/µL, WBC from 3.7×10(3)/µL to 7.7×10(3)/µL and NEUT from 46.0% to 78.3%. From the patient feelings and blood reports it showed that Carica papaya leaves aqueous extract exhibited potential activity against Dengue fever. Furthermore, the different parts of this valuable specie can be further used as a strong natural candidate against viral diseases.

  13. A polymorphic pseudoautosomal boundary in the Carica papaya sex chromosomes.

    Science.gov (United States)

    Lappin, Fiona M; Medert, Charles M; Hawkins, Kevin K; Mardonovich, Sandra; Wu, Meng; Moore, Richard C

    2015-08-01

    Sex chromosomes are defined by a non-recombining sex-determining region (SDR) flanked by one or two pseudoautosomal regions (PARs). The genetic composition and evolutionary dynamics of the PAR is also influenced by its linkage to the differentiated non-recombining SDR; however, understanding the effects of this linkage requires a precise definition of the PAR boundary. Here, we took a molecular population genetic approach to further refine the location of the PAR boundary of the evolutionary young sex chromosomes of the tropical plant, Carica papaya. We were able to map the position of the papaya PAR boundary A to a 100-kb region between two genetic loci approximately 2 Mb upstream of the previously genetically identified PAR boundary. Furthermore, this boundary is polymorphic within natural populations of papaya, with an approximately 100-130 kb expansion of the non-recombining SDR found in 16 % of individuals surveyed. The expansion of the PAR boundary in one Y haplotype includes at least one additional gene. Homologs of this gene are involved in male gametophyte and pollen development in other plant species.

  14. Identification of a new phospholipase D in Carica papaya latex.

    Science.gov (United States)

    Abdelkafi, Slim; Abousalham, Abdelkarim; Fendri, Imen; Ogata, Hiroyuki; Barouh, Nathalie; Fouquet, Benjamin; Scheirlinckx, Frantz; Villeneuve, Pierre; Carrière, Frédéric

    2012-05-15

    Phospholipase D (PLD) is a lipolytic enzyme involved in signal transduction, vesicle trafficking and membrane metabolism. It catalyzes the hydrolysis and transphosphatidylation of glycerophospholipids at the terminal phosphodiester bond. The presence of a PLD in the latex of Carica papaya (CpPLD1) was demonstrated by transphosphatidylation of phosphatidylcholine (PtdCho) in the presence of 2% ethanol. Although the protein could not be purified to homogeneity due to its presence in high molecular mass aggregates, a protein band was separated by SDS-PAGE after SDS/chloroform-methanol/TCA-acetone extraction of the latex insoluble fraction. This material was digested with trypsin and the amino acid sequences of the tryptic peptides were determined by micro-LC/ESI/MS/MS. These sequences were used to identify a partial cDNA (723 bp) from expressed sequence tags (ESTs) of C. papaya. Based upon EST sequences, a full-length gene was identified in the genome of C. papaya, with an open reading frame of 2424 bp encoding a protein of 808 amino acid residues, with a theoretical molecular mass of 92.05 kDa. From sequence analysis, CpPLD1 was identified as a PLD belonging to the plant phosphatidylcholine phosphatidohydrolase family.

  15. The potential of papaya leaf extract in controlling Ganoderma boninense

    Science.gov (United States)

    Tay, Z. H.; Chong, K. P.

    2016-06-01

    Basal Stem Rot (BSR) disease causes significant losses to the oil palm industry. Numerous controls have been applied in managing the disease but no conclusive result was reported. This study investigated the antifungal potential of papaya leaf extracts against Ganoderma boninense, the causal pathogen of BSR. Among the five different solvents tested in extraction of compounds from papaya leaf, methanol and acetone gave the highest yield. In vitro antifungal activity of the methanol and acetone extracts were evaluated against G. boninense using agar dilution at four concentrations: 5 mg mL-1, 15 mg mL-1, 30 mg mL-1and 45 mg mL-1. The results indicated a positive correlation between the concentration of leaf extracts and the inhibition of G. boninense. ED50 of methanol and acetone crude extracts were determined to be 32.016 mg mL-1and 65.268 mg mL-1, respectively. The extracts were later semi-purified using solid phase extraction (SPE) and the nine bioactive compounds were identified: decanoic acid, 2-methyl-, Z,Z-10-12-Hexadecadien-1-ol acetate, dinonanoin monocaprylin, 2-chloroethyl oleate, phenol,4-(1-phenylethyl)-, phenol,2,4-bis(1-phenylethyl)-, phenol-2-(1-phenylethyl)-, ethyl iso-allocholate and 1- monolinoleoylglycerol trimethylsilyl ether. The findings suggest that papaya leaf extracts have the ability to inhibit the growth of G. boninense, where a higher concentration of the extract exhibits better inhibition effects.

  16. Efecto de la madurez, geometría y presión sobre la cinética de transferencia de masa en la deshidratación osmótica de papaya (Carica papaya L., var. Maradol)

    OpenAIRE

    Lina Marcela Chavarro-Castrillón; Claudia Isabel Ochoa-Martínez; Alfredo Ayala-Aponte

    2006-01-01

    Las papayas obtenidas en el raleo (papayas menos desarrolladas entresacadas de los árboles para beneficiar el crecimiento de las otras) generalmente se descartan. Alternativamente, estas papayas pueden secarse por un proceso de deshidratación osmótica y secado térmico convencional para usarlas como snacks o como ingrediente para otros productos. Se comparó la cinética de transferencia de masa en la deshidratación osmótica de papayas de raleo frente a papayas desarrolladas con diferente grado ...

  17. Bio-safety of transgenic papaya resistant against Papaya ring spot virus%抗病毒转基因番木瓜及其安全性问题

    Institute of Scientific and Technical Information of China (English)

    李世访

    2011-01-01

    本文对番木瓜生产上存在的病害问题、转基因番木瓜的研发、转基因番木瓜国际竞争的动态与趋势、转基因番木瓜的安全性、转基因木瓜的标识等几个方面进行综述.%This paper was focused on the following issues: 1) Diseases of papaya; 2) Research and development of transgenic papaya; 3) Trends of international competition on transgenic papaya; 4) Safety of transgenic papaya; 5) Identification of transgenic papaya.

  18. Reguladores vegetais na quebra da dominância apical de mamoeiro (Carica papaya L. Plant growth regulators on breaking apical dominance in papaya plants (Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Elizabeth Orika Ono

    2004-08-01

    Full Text Available O trabalho avaliou os efeitos de reguladores vegetais sobre a quebra da dominância apical de mamoeiro (Carica papaya L. cv. Improved Sunrise Solo. A aplicação dos reguladores vegetais foi iniciada quando as plantas tinham seis meses de idade, totalizando três aplicações, a intervalos de sete dias, constando dos seguintes tratamentos: T1- água (testemunha; T2- GA3 250 mg L-1; T3- GA3 500 mg L-1; T4- benziladenina (BA 250 mg L-1; T5- BA 500 mg L-1; T6- GA3 125 mg L-1 + BA 125 mg L-1; T7- GA3 250 mg L-1 + BA 250 mg L-1. Esses tratamentos foram acompanhados da remoção ou não da gema apical. Os resultados mostraram que plantas tratadas com GA3 + BA a 125 e 250 mg L-1, com e sem a remoção da gema apical, apresentaram maior número de brotações que a testemunha, a qual não apresentou nenhuma brotação das gemas laterais.The objective of this work was to study the effects of gibberelin and cytokinin on breaking apical dominance and axillary buds growth of the Carica papaya L. Papaya plants sprayed three times within a period of 7 days, with the following treatments: T1- water (control, T2- GA3 250 mg L-1, T3- GA3 500 mg L-1, T4- benzyladenine (BA 250 mg L-1, T5- BA 500 mg L-1, T6- GA3 125 mg L-1 + BA 125 mg L-1, T7- GA3 250 mg L-1 + BA 250 mg L-1. The treatments were carried out with or without removal of the apical buds. After 36 days of the last plant growth regulators application was observed lateral bud numbers, lateral buds length and lateral buds diameter. The results showed that there were a higher number of shoots in plants treated with GA3 at 125 mg L-1 + BA at 125 mg L-1 and GA3 at 250mg L-1 + BA at 250 mg L-1. The removal of the apical bud helped the sprouting of the papaya plants.

  19. Hypoglycemic effect of Carica papaya leaves in streptozotocin-induced diabetic rats

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    Juárez-Rojop Isela Esther

    2012-11-01

    Full Text Available Abstract Background Traditional plant treatment for diabetes has shown a surging interest in the last few decades. Therefore, the purpose of this study was to assess the hypoglycemic effect of the aqueous extract of C. papaya leaves in diabetic rats. Several studies have reported that some parts of the C. papaya plant exert hypoglycemic effects in both animals and humans. Methods Diabetes was induced in rats by intraperitoneal administration of 60 mg/kg of streptozotocin (STZ. The aqueous extract of C. papaya was administered in three different doses (0.75, 1.5 and 3 g/100 mL as drinking water to both diabetic and non-diabetic animals during 4 weeks. Results The aqueous extract of Carica papaya (0.75 g and 1.5 g/100 mL significantly decreased blood glucose levels (pC. papaya could help islet regeneration manifested as preservation of cell size. In the liver of diabetic treated rats, C. papaya prevented hepatocyte disruption, as well as accumulation of glycogen and lipids. Finally, an antioxidant effect of C. papaya extract was also detected in diabetic rats. Conclusions This study showed that the aqueous extract of C. papaya exerted a hypoglycemic and antioxidant effect; it also improved the lipid profile in diabetic rats. In addition, the leaf extract positively affected integrity and function of both liver and pancreas.

  20. A Current Overview of the Papaya meleira virus, an Unusual Plant Virus

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    Paolla M. V. Abreu

    2015-04-01

    Full Text Available Papaya meleira virus (PMeV is the causal agent of papaya sticky disease, which is characterized by a spontaneous exudation of fluid and aqueous latex from the papaya fruit and leaves. The latex oxidizes after atmospheric exposure, resulting in a sticky feature on the fruit from which the name of the disease originates. PMeV is an isometric virus particle with a double-stranded RNA (dsRNA genome of approximately 12 Kb. Unusual for a plant virus, PMeV particles are localized on and linked to the polymers present in the latex. The ability of the PMeV to inhabit such a hostile environment demonstrates an intriguing interaction of the virus with the papaya. A hypersensitivity response is triggered against PMeV infection, and there is a reduction in the proteolytic activity of papaya latex during sticky disease. In papaya leaf tissues, stress responsive proteins, mostly calreticulin and proteasome-related proteins, are up regulated and proteins related to metabolism are down-regulated. Additionally, PMeV modifies the transcription of several miRNAs involved in the modulation of genes related to the ubiquitin-proteasome system. Until now, no PMeV resistant papaya genotype has been identified and roguing is the only viral control strategy available. However, a single inoculation of papaya plants with PMeV dsRNA delayed the progress of viral infection.

  1. Detection of Papaya leaf distortion mosaic virus by reverse-transcription loop-mediated isothermal amplification.

    Science.gov (United States)

    Shen, Wentao; Tuo, Decai; Yan, Pu; Li, Xiaoying; Zhou, Peng

    2014-01-01

    Papaya leaf distortion mosaic virus (PLDMV) can infect transgenic papaya resistant to a related pathogen, Papaya ringspot virus (PRSV), posing a substantial threat to papaya production in China. Current detection methods, however, are unable to be used for rapid detection in the field. Here, a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of PLDMV, using a set of four RT-LAMP primers designed based on the conserved sequence of PLDMV CP. The RT-LAMP method detected specifically PLDMV and was highly sensitive, with a detection limit of 1.32×10(-6) μg of total RNA per reaction. Indeed, the reaction was 10 times more sensitive than one-step RT-PCR, while also requiring significantly less time and equipment. The effectiveness of RT-LAMP and one-step RT-PCR in detecting the virus were compared using 90 field samples of non-transgenic papaya and 90 field samples of commercialized PRSV-resistant transgenic papaya from Hainan Island. None of the non-transgenic papaya tested positive for PLDMV using either method. In contrast, 19 of the commercialized PRSV-resistant transgenic papaya samples tested positive by RT-LAMP assay, and 6 of those tested negative by RT-PCR. Therefore, the PLDMV-specific RT-LAMP is a simple, rapid, sensitive, and cost-effective tool in the field diagnosis and control of PLDMV.

  2. Selection and testing of epiphytic yeasts to control anthacnose in post-harvest of papaya fruit

    NARCIS (Netherlands)

    Capdeville, G.; Souza, M.T.; Santos, J.R.P.; Miranda, S.P.; Caetano, A.R.; Torres, F.A.G.

    2007-01-01

    Anthracnose, caused by Colletotrichum gloeosporioides, is a major post-harvest disease in papaya fruit. The major objectives of the present work were to isolate, select and test the in vitro and in vivo ability of epiphytic microorganisms, isolated from papaya fruit and leaf surfaces, in controlling

  3. Release and establishment of Encarsia diaspidicola (Hymenoptera: Aphelididae) against white peach scale in papaya

    Science.gov (United States)

    White peach scale, Pseudaulacaspis pentagona (Hemiptera:Diaspididae) is a serious economic pest of papaya, Carica papaya L. The parasitic wasp Encarsia diaspidicola (Hymenoptera: Aphelinidae) was brought from Samoa into a quarantine containment facility in Hawaii for evaluation and potential release...

  4. Direct LAMP Assay without Prior DNA Purification for Sex Determination of Papaya.

    Science.gov (United States)

    Tsai, Chi-Chu; Shih, Huei-Chuan; Ko, Ya-Zhu; Wang, Ren-Huang; Li, Shu-Ju; Chiang, Yu-Chung

    2016-09-24

    Papaya (Carica papaya L.) is an economically important tropical fruit tree with hermaphrodite, male and female sex types. Hermaphroditic plants are the major type used for papaya production because their fruits have more commercial advantages than those of female plants. Sex determination of the seedlings, or during the early growth stages, is very important for the papaya seedling industry. Thus far, the only method for determining the sex type of a papaya at the seedling stage has been DNA analysis. In this study, a molecular technique-based on DNA analysis-was developed for detecting male-hermaphrodite-specific markers to examine the papaya's sex type. This method is based on the loop-mediated isothermal amplification (LAMP) and does not require prior DNA purification. The results show that the method is an easy, efficient, and inexpensive way to determine a papaya's sex. This is the first report on the LAMP assay, using intact plant materials-without DNA purification-as samples for the analysis of sex determination of papaya. We found that using high-efficiency DNA polymerase was essential for successful DNA amplification, using trace intact plant material as a template DNA source.

  5. An umbra-like virus of papaya discovered in Ecuador: detection, occurrence and phylogenetic relatedness

    Science.gov (United States)

    Double-stranded RNA (dsRNA) extractions from papaya leaves infected with Papaya ringspot virus (PRSV) revealed the presence of an unusual 4kb band, in addition to the presumed PRSV-associated 10kb band. Partial sequence of RT-PCR products from the 4kb dsRNA revealed homology to genomes of several me...

  6. Data on screening and identification of genetically modified papaya in food supplements

    NARCIS (Netherlands)

    Prins, Theo W.; Scholtens-Toma, Ingrid; Bak, Arno W.; Dijk, Van Jeroen P.; Voorhuijzen, Marleen M.; Laurensse, Emile J.; Kok, Esther J.

    2016-01-01

    This article contains data related to the research article entitled “A case study to determine the geographical origin of unknown GM papaya in routine food sample analysis, followed by identification of papaya events 16-0-1 and 18-2-4” (Prins et al., 2016) [1]. Quantitative real-time PCR (qPCR) with

  7. Effect of heat treatment on ethylene and CO2 emissions rates during papaya (Carica papaya L.) fruit ripening

    Science.gov (United States)

    da Silva, M. G.; Santos, E. O.; Sthel, M. S.; Cardoso, S. L.; Cavalli, A.; Monteiro, A. R.; de Oliveira, J. G.; Pereira, M. G.; Vargas, H.

    2003-01-01

    Ripening studies of nontreated and treated papaya (papaya L) are accomplished by monitoring the ethylene and CO2 emission rates of that climacteric fruit, to evaluate its shelf life. The treatments simulate the commercial Phitosanitarian process used to avoid the fly infestation. Ethylene emission was measured using a commercial CO2 laser driven photoacoustic setup and CO2, using a commercial gas analysis also based on the photothermal effect. The results show a marked change in ethylene and CO2 emission rate pattern for treated fruits when compared to the ones obtained for nontreated fruits and a displacement of the climacteric pick shown that the treatment causes a decrease of shelf life of fruit.

  8. Molecular characterization and pathogenicity of Erwinia spp. associated with pineapple [Ananas comosus (L. Merr.] and papaya (Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Ramachandran Kogeethavani

    2015-12-01

    Full Text Available The Erwinia species are well-known pathogens of economic importance in Malaysia causing serious damage to high-value fruit crops that include pineapple [Ananas comosus (L. Merr.] and papaya (Carica papaya L..The 16S rRNA sequence using eubacteria fD1 and rP2 primers, identified two bacteria species; Dickeya zeae from pineapple heart rot, and Erwinia mallotivora from papaya dieback. Phylogenetic analysis based on the neighbor-joining method indicated that all the bacterial isolates clustered in their own taxa and formed monophyletic clades. From the pathogenicity test, all isolates of D. zeae and E. mallotivora showed pathogenic reactions on their respective host plants. Genetic variability of these isolates was assessed using repetitive sequence-based PCR (rep-PCR fingerprinting. The results indicated interspecies, and intraspecies variation in both species’ isolates. There were more polymorphic bands shown by rep-PCR fingerprints than enterobacterial repetitive intergenic consensus (ERIC and BOX- PCRs, however both species’ isolates produced distinguishable banding patterns. Unweighted pair-group method with arithmetic averages (UPGMA cluster analysis indicated that all Dickeya and Erwinia isolates from the same species were grouped in the same main cluster. Similarity among the isolates ranged from 77 to 99%. Sequencing of 16S rRNA using eubacteria fD1 and rP2 primers, and rep-PCR fingerprinting revealed diversity among Dickeya and Erwinia isolates. But this method appears to be reliable for discriminating isolates from pineapple heart rot and papaya dieback.

  9. Histopathological changes in Wistar albino rats exposed to aqueous extract of unripe Carica papaya

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    Taofeeq Oduola

    2010-05-01

    Full Text Available Background: Exposure of animals to xenobiotics may or may not trigger adverse response at cellular levels. Aqueous extract of unripe Carica papaya is consumed by sickle cell patients as antisickling agent in Western Nigeria. Aim: This study was undertaken to investigate the effects of Carica papaya on certain organs in Wister albino rats exposed to aqueous extract of unripe Carica papaya. Materials and Methods: Different doses of aqueous extract of unripe Carica papaya were administered orally daily for 42 days to six groups of rats. At the end of exposure, the animals were sacrificed and tissue sections were prepared from livers, kidneys, hearts and small intestines using standard techniques. Results: Histopathological results showed that no pathological changes were observed in tissue sections of experimental animals when compared with tissue sections of the same organs in control animals. Conclusion: No pathological changes were elicited in the organs of rats exposed to aqueous extract of unripe Carica papaya.

  10. Creencias sobre Papanicolaou y cáncer cérvicouterino en un grupo de mujeres chilenas

    OpenAIRE

    Urrutia S,María Teresa

    2012-01-01

    Antecedentes: Son variadas las descripciones en relación a las creencias y como estas se relacionan con las conductas preventivas en cáncer cérvicouterino. Objetivo: Describir las creencias que tienen un grupo de mujeres chilenas pertenecientes al sistema público de atención acerca del Papanicolaou y cáncer cérvicouterino. Método: Estudio analítico de corte transversal realizado en 333 mujeres chilenas. Se estudiaron las creencias sobre el Papanicolaou y el cáncer cérvicouterino con un instru...

  11. Genome sequence comparison reveals a candidate gene involved in male-hermaphrodite differentiation in papaya (Carica papaya) trees.

    Science.gov (United States)

    Ueno, Hiroki; Urasaki, Naoya; Natsume, Satoshi; Yoshida, Kentaro; Tarora, Kazuhiko; Shudo, Ayano; Terauchi, Ryohei; Matsumura, Hideo

    2015-04-01

    The sex type of papaya (Carica papaya) is determined by the pair of sex chromosomes (XX, female; XY, male; and XY(h), hermaphrodite), in which there is a non-recombining genomic region in the Y and Y(h) chromosomes. This region is presumed to be involved in determination of males and hermaphrodites; it is designated as the male-specific region in the Y chromosome (MSY) and the hermaphrodite-specific region in the Y(h) chromosome (HSY). Here, we identified the genes determining male and hermaphrodite sex types by comparing MSY and HSY genomic sequences. In the MSY and HSY genomic regions, we identified 14,528 nucleotide substitutions and 965 short indels with a large gap and two highly diverged regions. In the predicted genes expressed in flower buds, we found no nucleotide differences leading to amino acid changes between the MSY and HSY. However, we found an HSY-specific transposon insertion in a gene (SVP like) showing a similarity to the Short Vegetative Phase (SVP) gene. Study of SVP-like transcripts revealed that the MSY allele encoded an intact protein, while the HSY allele encoded a truncated protein. Our findings demonstrated that the SVP-like gene is a candidate gene for male-hermaphrodite determination in papaya.

  12. Heat shock and gamma radiation in the quarantine process of Papaya CV. Solo(Carica papaya L.)

    Energy Technology Data Exchange (ETDEWEB)

    Pimentel, Rodrigo M.A.; Walder, Julio M.M. [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil)]. E-mails: rmapiment@cena.usp.br; jmwalder@cena.usp.br; Marcondes, Yvens E.M. [Universidade Metodista de Piracicaba (UNIMEP), SP (Brazil)]. E-mail: yvensmar@unimep.br

    2007-07-01

    Full text: To export, it is not only necessary to achieve quality standards, but also, quarantine standards. The main function of irradiation is quarantine control of fruit flies, therefore it is necessary to combine with treatments which focus at keeping fruit quality. The objective of this work was the evaluation of the quality of papaya submitted to heat treatment followed by irradiation at quarantine doses. Papayas were selected green and separated in treatments: a) control; irradiation at doses; b) 250, c) 500 Gy; d) hot water at 60 deg C for 30 seconds; and combined to doses e) 250, f) 500 Gy. The papayas were stored at 21 ({+-} 1 deg C) with relative humidity of 85 - 90%, and then after 8 days, they were evaluated by their soluble solid content, pH, titratable acidity, firmness, decay index and internal color. The analysis of mass loss and external color were executed at 0, 2, 4 and 7 days after irradiation. It was observed that in fruits submitted to hot water (d, e, f) there were less decay and in combined treatments (e, f) it was verified higher firmness. In the second day, irradiated treatments (b, c, e, f) were more yellow than the others. As to the other parameters evaluated, there were no differences between treatments (author)

  13. Implementación del enfoque de derechos humanos: la reforma sanitaria chilena

    OpenAIRE

    Nuria Cunill-Grau; Margarita Fernández

    2014-01-01

    EI propósito de este trabajo es analizar la incidencia de la arquitectura institucional publica en la implementación de las políticas públicas con enfoque de derechos humanos. Se adopta como caso de estudio la reforma sanitaria chilena a partir del año 2005, que reconoce garantías exigibles de acceso, oportunidad, calidad y protección financiera para un conjunto de problemas de salud a la ciudadanía, independientemente de su adscripción al sistema público 0 privado. La metodología integró un ...

  14. FACTORES DETERMINANTES DEL ENDEUDAMIENTO BANCARIO EN LA EMPRESA NO FINANCIERA CHILENA

    OpenAIRE

    Mauricio Jara Bertin; Sebastián Sánchez Chavarría

    2012-01-01

    El objetivo de este artículo es constatar si la elección de recurrir a la banca como fuente de financiación viene determinada por las oportunidades de crecimiento, ta-maño de la empresa, calidad de los proyectos, la calidad de las garantías prendarias y el riesgo de la empresa. Hemos utilizado una muestra de 168 empresas chilenas cotizadas en la Bolsa durante el periodo 1997-2008, que nos ha servido para exami-nar la relación entre factores específicos de las empresas y el endeudamiento ban-c...

  15. Referentes internacionales para el giro reformista de la izquierda chilena (1975-1990

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    Marcelo Mella Polanco

    2011-01-01

    Full Text Available El presente artículo analiza el giro reformista de la izquierda chilena durante el régimen autoritario de Pinochet. Para lograrlo, se expone cómo la penetración de ideas provenientes del marxismo analítico homogeneizó un importante sector de intelectuales y expertos opositores de Pinochet, constituyendo, de esta forma, un nuevo mainstream político-académico capaz de renovar los marcos epistémicos de las ciencias sociales y diseñar la transición a la democracia.

  16. AUTORIAS FEMENINAS FUNDACIONALES: ESCRITORAS CHILENAS Y BRASILEÑAS DEL SIGLO XIX (1840-1890)

    OpenAIRE

    ARCOS HERRERA, CAROL

    2013-01-01

    La siguiente investigación tiene por objetivo general estudiar el problemático ingreso de las escritoras chilenas y brasileñas en la comunicación e institucionalidad literaria de sus respectivos países entre 1840 y 1890. Su hipótesis de lectura dice relación con entender la emergencia de estas escritoras desde el punto de vista de su autoría, de su construcción como sujetos modernos de escritura, a partir del soporte impreso más utilizado en la época como lo es la prensa. Así, entonces, se...

  17. La iglesia chilena a fines del siglo XIX: un paradigma pastoral

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    Camus Ibacache, Misael

    2002-06-01

    Full Text Available Study of the training and consolidation of the eclesiastic structure Chilean during the XlXth century and the two first decades of the XXth century through the documents of the visits ad liminina, the pastoral visits and the resolutions of the Episcopal Meetings.

    Estudio de la formación y consolidación de la estructura eclesiástica chilena durante el siglo XIX y las dos primeras décadas del XX a través de los documentos de las visitas ad liminina, las visitas pastorales y las Actas de las Sesiones Episcopales.

  18. Contrasting patterns of X/Y polymorphism distinguish Carica papaya from other sex chromosome systems.

    Science.gov (United States)

    Weingartner, Laura A; Moore, Richard C

    2012-12-01

    The sex chromosomes of the tropical crop papaya (Carica papaya) are evolutionarily young and consequently allow for the examination of evolutionary mechanisms that drive early sex chromosome divergence. We conducted a molecular population genetic analysis of four X/Y gene pairs from a collection of 45 wild papaya accessions. These population genetic analyses reveal striking differences in the patterns of polymorphism between the X and Y chromosomes that distinguish them from other sex chromosome systems. In most sex chromosome systems, the Y chromosome displays significantly reduced polymorphism levels, whereas the X chromosome maintains a level of polymorphism that is comparable to autosomal loci. However, the four papaya sex-linked loci that we examined display diversity patterns that are opposite this trend: the papaya X alleles exhibit significantly reduced polymorphism levels, whereas the papaya Y alleles maintain greater than expected levels of diversity. Our analyses suggest that selective sweeps in the regions of the X have contributed to this pattern while also revealing geographically restricted haplogroups on the Y. We discuss the possible role sexual selection and/or genomic conflict have played in shaping the contrasting patterns of polymorphism found for the papaya X and Y chromosomes.

  19. Wound-healing potential of an ethanol extract of Carica papaya (Caricaceae) seeds.

    Science.gov (United States)

    Nayak, Bijoor Shivananda; Ramdeen, Ria; Adogwa, Andrew; Ramsubhag, Adash; Marshall, Julien Rhodney

    2012-12-01

    Carica papaya L. (Linn) (Caricaceae) is traditionally used to treat various skin disorders, including wounds. It is widely used in developing countries as an effective and readily available treatment for various wounds, particularly burns. This study evaluated the wound-healing and antimicrobial activity of C. papaya seed extract. Ethanol extract of C. papaya seed (50 mg/kg/day) was evaluated for its wound-healing activity in Sprague-Dawley rats using excision wound model. Animals were randomly divided into four groups of six each (group 1 served as control, group 2 treated with papaya seed extract, group 3 treated with a standard drug mupirocin and papaya seed extract (1:1 ratio) and group 4 treated with a mupirocin ointment. Rate of wound contraction and hydroxyproline content were determined to assess the wound-healing activity of the seed extract. The group 2 animals showed a significant decrease in wound area of 89% over 13 days when compared with groups 1 (82%), 3 (86%) and 4 (84%) respectively. The hydroxyproline content was significantly higher with the granulation tissue obtained from group 2 animals which were treated with C. papaya seed extract. Histological analysis of granulation tissue of the group 2 animals showed the deposition of well-organized collagen. The extract exhibited antimicrobial activity against Salmonella choleraesuis and Staphylococcus aureus. Our results suggest that C. papaya promotes significant wound healing in rats and further evaluation for this activity in humans is suggested.

  20. The Evolutionary Tempo of Sex Chromosome Degradation in Carica papaya.

    Science.gov (United States)

    Wu, Meng; Moore, Richard C

    2015-06-01

    Genes on non-recombining heterogametic sex chromosomes may degrade over time through the irreversible accumulation of deleterious mutations. In papaya, the non-recombining male-specific region of the Y (MSY) consists of two evolutionary strata corresponding to chromosomal inversions occurring approximately 7.0 and 1.9 MYA. The step-wise recombination suppression between the papaya X and Y allows for a temporal examination of the degeneration progress of the young Y chromosome. Comparative evolutionary analyses of 55 X/Y gene pairs showed that Y-linked genes have more unfavorable substitutions than X-linked genes. However, this asymmetric evolutionary pattern is confined to the oldest stratum, and is only observed when recently evolved pseudogenes are included in the analysis, indicating a slow degeneration tempo of the papaya Y chromosome. Population genetic analyses of coding sequence variation of six Y-linked focal loci in the oldest evolutionary stratum detected an excess of nonsynonymous polymorphism and reduced codon bias relative to autosomal loci. However, this pattern was also observed for corresponding X-linked loci. Both the MSY and its corresponding X-specific region are pericentromeric where recombination has been shown to be greatly reduced. Like the MSY region, overall selective efficacy on the X-specific region may be reduced due to the interference of selective forces between highly linked loci, or the Hill-Robertson effect, that is accentuated in regions of low or suppressed recombination. Thus, a pattern of gene decay on the X-specific region may be explained by relaxed purifying selection and widespread genetic hitchhiking due to its pericentromeric location.

  1. Papaya pulp gelling: is it premature ripening or problems of water accumulation in the apoplast?

    Directory of Open Access Journals (Sweden)

    Jurandi Gonçalves de Oliveira

    Full Text Available Gelled aspect in papaya fruit is typically confused with premature ripening. This research reports the characterization of this physiological disorder in the pulp of papaya fruit by measuring electrolyte leakage, Pi content, lipid peroxidation, pulp firmness, mineral contents (Ca, Mg and K - in pulp and seed tissues, and histological analysis of pulp tissue. The results showed that the gelled aspect of the papaya fruit pulp is not associated with tissue premature ripening. Data indicate a reduction of the vacuole water intake as the principal cause of the loss of cellular turgor; while the waterlogged aspect of the tissue may be due to water accumulation in the apoplast.

  2. Estudio de una mutación en papaya (Carica papaya L. que produce letalidad de plantas femeninas

    Directory of Open Access Journals (Sweden)

    Eric Mora Newcomer

    2005-01-01

    Full Text Available Estudio de una mutación en papaya (Carica papayaL. que produce letalidad de plantas femeninas. Durante elperíodo 1995-2003 se descubrió y estudió una nueva mutaciónen una línea genética de papaya. En esta especie, las plantashermafroditas son heterocigotos obligadas debido a la letalidad de la condición homocigota para el alelo del hermafroditismo, el cual es a su vez dominante sobre el alelo femenino.Por este motivo, la segregación de una planta hermafrodita sigue una relación 2:1 (hermafroditas:femeninas. En este trabajo, se reporta la aparición de una línea mutante en la cual elporcentaje de plantas hermafroditas y femeninas es de 95% y5% respectivamente, lo que sugiere la aparición una mutaciónasociado al alelo femenino que también provoca la letalidadde este genotipo en condición homocigótica. La hibridaciónentre una planta femenina con una planta hermafrodita de esta línea produce una descendencia que sigue una relación de2:1 (hermafroditas:femeninas en lugar del esperado 1:1. Esteresultado sugiere que las pocas plantas femeninas de esta líneason el resultado de la reversión de esta letalidad en uno de susalelos, lo que les permite recobrar su viabilidad. Esta líneapresenta también un porcentaje bajo de plantas hermafroditascon diversas anomalías en sus flores. Se sugiere que todas lasanomalías de esta línea son de origen epigenético, posiblemente causadas por transposones.

  3. Sales de calcio mejoran vida de anaquel y aceptabilidad general de papaya (Carica papaya L. var. Maradol fresca cortada

    Directory of Open Access Journals (Sweden)

    Nayely Leyva López

    2011-01-01

    Full Text Available Las tendencias actuales de los consumidores por alimentos sanos y de conveniencia promueven un mayor consumo de frutas y hortalizas, donde destacan productos frescos cortados. Sin embargo, por su naturaleza estos productos pueden ser muy susceptibles a diferentes alteraciones que afectan su calidad. El uso de aditivos como sales de calcio representa una alternativa tecnológica para mantener, o incluso mejorar, la calidad durante su vida de anaquel. Por lo anterior el objetivo de esta investigación fue evaluar el efecto de sales de calcio en papaya fresca cortada sobre la calidad física, química, microbiológica, nutracéutica y sensorial. Cubos de papaya, variedad Maradol (2 cm de arista fueron tratados con lactato y cloruro de calcio al 1,0 y 3,0 %, bajo condiciones de inmersión por 2 min a 40 °C. Las muestras tratadas fueron colocadas en vasos de polietileno tereftalato (PET de 500 mL con tapa, y almacenadas por 8 días a 5 °C. En comparación con los frutos testigo, los tratamientos a base de calcio demostraron mejorar la firmeza (~ 8 N, principalmente los frutos tratados con cloruro de calcio al 3,0 %. Por otra parte, la calidad nutracéutica (fenólicos totales no se vio afectada por el uso de sales de calcio, mientras que las evaluaciones microbiológicas demuestran condiciones de inocuidad conforme a normatividad. Se sugiere el uso de cloruro de calcio para fines de comercializar papaya fresca cortada.

  4. Sales de calcio mejoran vida de anaquel y aceptabilidad general de papaya (Carica papaya L. var. Maradol fresca cortada

    Directory of Open Access Journals (Sweden)

    Nayely Leyva López

    2011-06-01

    Full Text Available Las tendencias actuales de los consumidores por alimentos sanos y de conveniencia promueven un mayor consumo de frutas y hortalizas, donde destacan productos frescos cortados. Sin embargo, por su naturaleza estos productos pueden ser muy susceptibles a diferentes alteraciones que afectan su calidad. El uso de aditivos como sales de calcio representa una alternativa tecnológica para mantener, o incluso mejorar, la calidad durante su vida de anaquel. Por lo anterior el objetivo de esta investigación fue evaluar el efecto de sales de calcio en papaya fresca cortada sobre la calidad física, química, microbiológica, nutracéutica y sensorial. Cubos de papaya, variedad Maradol (2 cm de arista fueron tratados con lactato y cloruro de calcio al 1,0 y 3,0 %, bajo condiciones de inmersión por 2 min a 40 °C. Las muestras tratadas fueron colocadas en vasos de polietileno tereftalato (PET de 500 mL tapa, y almacenadas por 8 días a 5 °C. En comparación con los frutos testigo, los tratamientos a base de calcio demostraron mejorar la firmeza (~ 8 N, principalmente los frutos tratados con cloruro de calcio al 3,0 %. Por otra parte, la calidad nutracéutica (fenólicos totales no se vio afectada por el uso de sales de calcio, mientras que las evaluaciones microbiológicas demuestran condiciones de inocuidad conforme a normatividad. Se sugiere el uso de cloruro de calcio para fines de comercializar papaya fresca cortada.

  5. Dengue fever treatment with Carica papaya leaves extracts

    Institute of Scientific and Technical Information of China (English)

    Nisar Ahmad; Hina Fazal; Muhammad Ayaz; Bilal Haider Abbasi; Ijaz Mohammad; Lubna Fazal

    2011-01-01

    The main objective of the current study is to investigate the potential of Carica papaya leaves extracts against Dengue fever in 45 year old patient bitten by carrier mosquitoes. For the treatment of Dengue fever the extract was prepared in water. 25 mL of aqueous extract of C. papaya leaves was administered to patient infected with Dengue fever twice daily i.e. morning and evening for five consecutive days. Before the extract administration the blood samples from patient were analyzed. Platelets count (PLT), White Blood Cells (WBC) and Neutrophils (NEUT) decreased from 176×103/μL, 8.10×10 3/μL, 84.0% to 55×10 3/μL, 3.7×10 3/μL and 46.0%. Subsequently, the blood samples were rechecked after the administration of leaves extract. It was observed that the PLT count increased from 55×103/μL to 168×10 3/μL, WBC from 3.7×10 3/μL to 7.7×10 3/μL and NEUT from 46.0% to 78.3%. From the patient feelings and blood reports it showed that Caricapapaya leaves aqueous extract exhibited potential activity against Dengue fever. Furthermore, the different parts of this valuable specie can be further used as a strong natural candidate against viral diseases.

  6. Methanolysis of Carica papaya Seed Oil for Production of Biodiesel

    Directory of Open Access Journals (Sweden)

    Foluso O. Agunbiade

    2014-01-01

    Full Text Available The future of fossil fuel sources of energy has necessitated the need to search for renewable alternatives. Thus, Carica papaya seed oil (CPSO was employed as feedstock for the production of biodiesel by methanolysis. The seed was obtained locally, dried, and extracted with n-hexane. The CPSO was analyzed for specific gravity, viscosity, iodine value, and saponification value, among others using standard methods. The oil was transesterified by two-stage catalysis with oil to methanol mole ratio of 1 : 9. The biodiesel produced was subjected to standard fuel tests. The seed has an oil yield of 31.2% which is commercially viable. The kinematic viscosity of the oil at 313 K was 27.4 mm2s−1 while that of Carica papaya oil methylester (CPOME was reduced to 3.57 mm2s−1 and the specific gravity was 0.84 comparable with other seed-oil biodiesels and number 2 diesel. Other oil properties were compared favourably with seed oils already documented for biodiesel synthesis. CPOME’s cloud and pour points were 275 K and 274 K, respectively, and relatively higher than other biodiesels and number 2 diesel. CPOME exhibits moderate corrosion of copper strip. The methanolysis improved the fuel properties of the CPOME similar to other biodiesels. CPSO therefore exhibits a potential for biodiesel production.

  7. Immobilization of mycoplana sp. MVMB2 isolated from petroleum contaminated soil onto papaya stem (carica papaya l.) and its application on degradation of phenanthrene

    Energy Technology Data Exchange (ETDEWEB)

    Brinda Lakshmi, Mahalingam; Muthukumar, Karuppan; Velan, Manickam [Environmental Biotechnology Laboratory, Department of Chemical Engineering, A.C. College of Technology, Anna University, Chennai (India)

    2012-08-15

    This study presents the degradation of phenanthrene using immobilized Mycoplana sp. MVMB2 isolated from contaminated soil. Papaya stem pretreated by two stage processes, treating with acid or alkali and drying, was used for the immobilization of Mycoplana sp. Alkali pretreated papaya stem was found to be most effective in cell uptake compared to acid treated one. The maximum immobilization capacity at various physiochemical conditions for the alkali pretreated papaya stem was found to be at 320 min time, pH 6.5, 30 C temperature, and 18.6 x 10{sup 6} cells/mL initial concentrations. The adsorption mechanism of Mycoplana sp. MVMB2 on pretreated papaya stem was assessed using various kinetic and isotherm models. The immobilization of Mycoplana sp. MVMB2 on to pretreated papaya stem was corroborated by scanning electron microscopy and Fourier transformed IR spectroscopy analysis. The performance of immobilized cells in batch reactor showed more than 95% phenanthrene degradation within 72 h, whereas, free cells were found to require 120 h. The immobilized cells also showed better degradation performance in the packed column study. (Copyright copyright 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  8. Analysis of Papaya Cell Wall-Related Genes during Fruit Ripening Indicates a Central Role of Polygalacturonases during Pulp Softening

    Science.gov (United States)

    Fabi, João Paulo; Broetto, Sabrina Garcia; da Silva, Sarah Lígia Garcia Leme; Zhong, Silin; Lajolo, Franco Maria; do Nascimento, João Roberto Oliveira

    2014-01-01

    Papaya (Carica papaya L.) is a climacteric fleshy fruit that undergoes dramatic changes during ripening, most noticeably a severe pulp softening. However, little is known regarding the genetics of the cell wall metabolism in papayas. The present work describes the identification and characterization of genes related to pulp softening. We used gene expression profiling to analyze the correlations and co-expression networks of cell wall-related genes, and the results suggest that papaya pulp softening is accomplished by the interactions of multiple glycoside hydrolases. The polygalacturonase cpPG1 appeared to play a central role in the network and was further studied. The transient expression of cpPG1 in papaya results in pulp softening and leaf necrosis in the absence of ethylene action and confirms its role in papaya fruit ripening. PMID:25162506

  9. Complete nucleotide sequence of a monopartite Begomovirus and associated satellites infecting Carica papaya in Nepal.

    Science.gov (United States)

    Shahid, M S; Yoshida, S; Khatri-Chhetri, G B; Briddon, R W; Natsuaki, K T

    2013-06-01

    Carica papaya (papaya) is a fruit crop that is cultivated mostly in kitchen gardens throughout Nepal. Leaf samples of C. papaya plants with leaf curling, vein darkening, vein thickening, and a reduction in leaf size were collected from a garden in Darai village, Rampur, Nepal in 2010. Full-length clones of a monopartite Begomovirus, a betasatellite and an alphasatellite were isolated. The complete nucleotide sequence of the Begomovirus showed the arrangement of genes typical of Old World begomoviruses with the highest nucleotide sequence identity (>99 %) to an isolate of Ageratum yellow vein virus (AYVV), confirming it as an isolate of AYVV. The complete nucleotide sequence of betasatellite showed greater than 89 % nucleotide sequence identity to an isolate of Tomato leaf curl Java betasatellite originating from Indonesian. The sequence of the alphasatellite displayed 92 % nucleotide sequence identity to Sida yellow vein China alphasatellite. This is the first identification of these components in Nepal and the first time they have been identified in papaya.

  10. Assessment of the anti-protozoal activity of crude Carica papaya seed extract against Trypanosoma cruzi.

    Science.gov (United States)

    Jiménez-Coello, Matilde; Guzman-Marín, Eugenia; Ortega-Pacheco, Antonio; Perez-Gutiérrez, Salud; Acosta-Viana, Karla Y

    2013-10-11

    In order to determine the in vivo activity against the protozoan Trypanosoma cruzi, two doses (50 and 75 mg/kg) of a chloroform extract of Carica papaya seeds were evaluated compared with a control group of allopurinol. The activity of a mixture of the three main compounds (oleic, palmitic and stearic acids in a proportion of 45.9% of oleic acid, 24.1% of palmitic and 8.52% of stearic acid previously identified in the crude extract of C. papaya was evaluated at doses of 100, 200 and 300 mg/kg. Both doses of the extracts were orally administered for 28 days. A significant reduction (p papaya extract in comparison with the positive control group (allopurinol 8.5 mg/kg). Parasitemia in animals treated with the fatty acids mixture was also significantly reduced (p papaya (from ripe fruit) are able to reduce the number of parasites from both parasite stages, blood trypomastigote and amastigote (intracellular stage).

  11. Analysis on virus resistance and fruit quality for T4 generation of transgenic papaya

    Institute of Scientific and Technical Information of China (English)

    WEI Xiangdong; LAN Congyu; LU Zhijing; YE Changming

    2007-01-01

    Molecular biological characterization,fruit characters,and nutrients were analyzed for T4 generation of transgenic papaya.All transgenic papaya plants with the mutated replicase (RP) gene from papaya ringspot virus (PRSV) showed high resistance or immunity against PRSV in the field.The RP transgene can be steadily inherited to,and expressed at RNA level,the progenies.The growth characteristics of transgenic papaya were much better than nontransgenic papaya in the field.The non-transgenic papaya seedlings began to show typical symptoms caused by PRSV after being inoculated with PRSV.They died quickly and never grew to produce fruit.The adult trees developed yellow leaves and produced smaller fruits and were doomed to a slow death after some time,while most oftransgenic papaya plants (about 91.8%) did not show any symptoms caused by PRSV,and produced more,bigger,and high quality fruits.Compared with non-transgenic plants,the fresh fruit length of T4 generation of transgenic papaya increased 2.6%-5%,and the diameter decreased 0.6%-1.5%.The flesh thickness of fresh fruit increased 12%-15%,which made it fitter for eating.Although the fresh fruit quality changed,there was no significant difference between transgenic and non-transgenic papaya.The quality characteristics of dry fruit including the contents of water,lipid,N,protein,reduced sugar,vitamin A,vitamin C,and carotene in the T4 generation of transgenic papaya were all the same as their non-transgenic parents.This means that transgenic plants and non-transgenic plants are substantially equivalent,and the transgene has no effect on dry fruit quality.In this study,we found that vitamin A and vitamin C in red-fleshed papaya were 1.4-1.8 and 1.78-2.07 times more than the yellow-fleshed ones,respectively,while N and protein were only 84.2%-92.1% and 82.1%-98.9% of the yellow-fleshed ones.

  12. Factors affecting the thermal activation of Neosartorya fischeri in pineapple and papaya nectars

    OpenAIRE

    Slongo, Adriana Paula; Aragão,Gláucia Maria Falcão de

    2006-01-01

    In this research factors that influence the activation of Neosartorya fischeri ascospores (heating medium, temperature of production and age of the ascospores) were studied. The heating medium used was pineapple and papaya nectars at different ratios (ºBrix/acidity). Lower activation times were observed for the N. fischeri ascospores in pineapple and papaya nectars under the conditions of temperature and production age of 25ºC for 1 month and using ratios of the heating medium of 10 for pinea...

  13. Turismo sostenible y proyectos hidroeléctricos: contradicciones en la patagonia chilena

    Directory of Open Access Journals (Sweden)

    Gabriel Inostroza

    2014-01-01

    Full Text Available El artículo analiza las perspectivas del turismo sostenible en la Patagonia chilena en relación a la probable implantación de centrales hidroeléctricas en su territorio. Se estudia el impacto de un gran proyecto hidroeléctrico en la región de Aysén (Chile y su repercusión en el turismo. Se concluye que la construcción y operación de grandes embalses y presas con sus correspondientes líneas de trasmisión eléctrica atentarían contra la principal ventaja competitiva del sector turístico de la Patagonia: una naturaleza que aún se conserva y que se ha proyectado en el imaginario social.

  14. Tendencias en la historiografía eclesiástica Chilena

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    Camus Ibacache, Misael

    2001-12-01

    Full Text Available The article studies the lines of Chilean ecclesiastical history in XIX and XX. During both centuries the studies are centered in: Independence, Church-State relations, missions, editions of sinodal and pastoral texts. The A. values the influence of the liberal historiography in ecclesiastical history and emphasizes the existence of a new current that denominates pastoral historiography and describes the new sources that are used.

    Estudia las corrientes de la historia eclesiástica chilena en los siglos XIX y XX. Durante ambos siglos los estudios se centran en la Independencia, relaciones Iglesia-Estado, misiones, ediciones de textos conciliares y ediciones de textos pastorales. El A. valora la influencia de la historiografía liberal en la historia eclesiástica y destaca la existencia de una nueva corriente que denomina historiografía pastoral y describe sus fuentes.

  15. Creencias y prácticas de literacidad en familias chilenas con distintos niveles educativos

    Directory of Open Access Journals (Sweden)

    Lissi María Rosa

    2007-01-01

    Full Text Available El estudio describe las creencias y prácticas de literacidad de familias con distinta educación en Chile. Participaron 188 niños y niñas de kindergarten y sus familias. Los padres contestaronun cuestionario acerca de su nivel educacional, ciertas prácticas de literacidcd familiar, y sus creencias acerca del desarrollo de ésta. Los resultados muestran diferencias y similitudes con resultados de países desarrollados. Las principales diferencias se dan en la frecuencia de ciertas prácticas, las que ocurren con menor frecuencia en las familias chilenas, independientemente del nivel educacional. Las principales similitudes con los hallazgos de países desarrollados se dan en las asociaciones entre el nivel educacional de la familia y las variables de literacidad familiar

  16. Implementación del enfoque de derechos humanos: la reforma sanitaria chilena

    Directory of Open Access Journals (Sweden)

    Nuria Cunill-Grau

    2014-01-01

    Full Text Available EI propósito de este trabajo es analizar la incidencia de la arquitectura institucional publica en la implementación de las políticas públicas con enfoque de derechos humanos. Se adopta como caso de estudio la reforma sanitaria chilena a partir del año 2005, que reconoce garantías exigibles de acceso, oportunidad, calidad y protección financiera para un conjunto de problemas de salud a la ciudadanía, independientemente de su adscripción al sistema público 0 privado. La metodología integró un análisis cualitativo de normas legales, instrumentos de gestión y entrevistas en profundidad principalmente a directivos de salud. Los resultados muestran que, no obstante los evidentes logros de la reforma chilena en pos de la equidad en salud, resultan limitados sus esfuerzos de dotar de gobernanza sistémica, accountability y espacio público a su arquitectura institucional, comprometiendo con ello su intención de instaurar un enfoque de derechos humanos. Se concluye que el patrón hegemónico de diseño institucional, organización y gestión de los servicios sociales requiere ser enfrentado para construirviabilidad a la implementación de este tipo de política y se sugiere la importancia de adoptar estrategias diferenciadas de accountabitily que consideren las asimetrías sociales en la apropiación y exigibilidad de los derechos.

  17. Symbiotic interactions between arbuscular mycorrhizal (AM) fungi and male papaya plants: its status, role and implications.

    Science.gov (United States)

    Khade, Sharda W; Rodrigues, Bernard F; Sharma, Prabhat K

    2010-01-01

    Experiments were conducted to study the arbuscular mycorrhizal (AM) status and its role in P-uptake through assay of root phosphatases activities in four varieties of male Carica papaya L. viz. CO-1, CO-2, Honey Dew and Washington during flowering stages. In the present study, mean total root colonization of AM fungi recorded peak increase in flowering stage-II while mean root phosphatase (acid and alkaline) activities recorded peak increase in flowering stage-I. Unlike root colonization and root phosphatase activities, spore density did not exhibit any definite patterns and recorded a narrow range of fluctuation during different flowering stages of male C. papaya. The study brought out the fact that root colonization and spore density of AM fungi along with root phosphatase activities varied significantly within the four varieties of male C. papaya plants during each flowering stage. The study also recorded consistently higher acid root phosphatase activity than alkaline root phosphatase activity under P-deficient, acidic soil conditions during all flowering stages of male C. papaya plants. Studies revealed that the root colonization of AM fungi influenced root phosphatase activities (acid and alkaline) positively and significantly during all flowering stages of male C. papaya plants. A total of twelve species of AM fungi belonging to five genera viz. Acaulospora, Dentiscutata, Gigaspora, Glomus, and Racocetra were recovered from the rhizosphere of male C. papaya plants.

  18. Variation in the Coat Protein Gene of Papaya ringspot Virus Isolates from Multiple Locations of China

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The potyvirus Papaya ringspot virus (PRSV) is an important pathogen of papaya that causes severe losses in economic crops for papaya production globally. The coat protein (CP) genes of five PRSV isolates originating from different locations in China were cloned and sequenced. The CP-coding region varied in size from 864-873 nucleotides, encoding proteins of 288-291 amino acids. The five Chinese isolates of PRSV have been characterized as papaya-infecting (PRSV-P). The CP sequences of the Chinese isolates were compared with those of previously published PRSV isolates originating from different countries at amino acid levels. A number of KE repeat boxes in the N terminus of the PRSV-CP were found in all Chinese isolates. The phylogenetic branching pattern revealed that there was certain extended grouping between geographic locations, and the Asian type probably represents the oldest population of PRSV. The information of CP genes will be useful in designing and developing durable virus resistant-PRSV transgenic papaya in China. Meanwhile broad-spectrum-virus resistant, strongly resistant-PRSV and good safe papaya lines are required.

  19. Oral dosing with papaya latex is an effective anthelmintic treatment for sheep infected with Haemonchus contortus

    Directory of Open Access Journals (Sweden)

    Donnan Alison A

    2011-03-01

    Full Text Available Abstract Background The cysteine proteinases in papaya latex have been shown to have potent anthelmintic properties in monogastric hosts such as rodents, pigs and humans, but this has not been demonstrated in ruminants. Methods In two experiments, sheep were infected concurrently with 5,000 infective larvae of Haemonchus contortus and 10,000 infective larvae of Trichostrongylus colubriformis and were then treated with the supernatant from a suspension of papaya latex from day 28 to day 32 post-infection. Faecal egg counts were monitored from a week before treatment until the end of the experiment and worm burdens were assessed on day 35 post-infection. Results We found that the soluble fraction of papaya latex had a potent in vivo effect on the abomasal nematode H. contortus, but not on the small intestinal nematode T. colubriformis. This effect was dose-dependent and at tolerated levels of gavage with papaya latex (117 μmol of active papaya latex supernatant for 4 days, the H. contortus worm burdens were reduced by 98%. Repeated treatment, daily for 4 days, was more effective than a single dose, but efficacy was not enhanced by concurrent treatment with the antacid cimetidine. Conclusions Our results provide support for the idea that cysteine proteinases derived from papaya latex may be developed into novel anthelmintics for the treatment of lumenal stages of gastro-intestinal nematode infections in sheep, particularly those parasitizing the abomasum.

  20. Direct LAMP Assay without Prior DNA Purification for Sex Determination of Papaya

    Directory of Open Access Journals (Sweden)

    Chi-Chu Tsai

    2016-09-01

    Full Text Available Papaya (Carica papaya L. is an economically important tropical fruit tree with hermaphrodite, male and female sex types. Hermaphroditic plants are the major type used for papaya production because their fruits have more commercial advantages than those of female plants. Sex determination of the seedlings, or during the early growth stages, is very important for the papaya seedling industry. Thus far, the only method for determining the sex type of a papaya at the seedling stage has been DNA analysis. In this study, a molecular technique—based on DNA analysis—was developed for detecting male-hermaphrodite-specific markers to examine the papaya’s sex type. This method is based on the loop-mediated isothermal amplification (LAMP and does not require prior DNA purification. The results show that the method is an easy, efficient, and inexpensive way to determine a papaya’s sex. This is the first report on the LAMP assay, using intact plant materials-without DNA purification-as samples for the analysis of sex determination of papaya. We found that using high-efficiency DNA polymerase was essential for successful DNA amplification, using trace intact plant material as a template DNA source.

  1. Empleo del método de secado convectivo combinado para la deshidratación de papaya (Carica papaya L.), variedad Maradol roja

    OpenAIRE

    Sahylin Muñiz Becerá; Antihus Hernández Gómez; Annia García Pereira; Lilia Méndez Lagunas

    2013-01-01

    La presente investigación tiene como objetivo evaluar el método de secado convectivo de papaya (Carica papaya L.) variedad Maradol roja, combinado con la aplicación de pretratamientos de osmosis (DOSC) y escaldado simple (ESSC), mediante el efecto de los factores tecnológi- cos del secador: temperatura (40 y 60oC) y velocidad del flujo de aire (2,5 y 1,5 m/s) sobre el comportamiento de las propiedades de calidad de la fruta deshidratada y la cinética del proceso. El pretratamiento de escaldad...

  2. EXPERIENCIA CHILENA CON EL USO DE UN ANTICONCEPTIVO ORAL COMBINADO DE ACETATO DE CLORMADINONA/ETINILESTRADIOL: ESTUDIO NO INTERVENCIONAL

    OpenAIRE

    2008-01-01

    Objetivos: Evaluar en mujeres chilenas, la eficacia, tolerabilidad y seguridad del anticonceptivo Belara® (Acetato de Clormadinona 2 mg y Etinilestradiol 0,03 mg) (ACM/EE). Métodos: Estudio no intervencional, multicéntrico, prospectivo, de 6 ciclos de seguimiento, evaluando efectividad anticonceptiva, estabilidad de ciclo, tolerabilidad, seguridad y acción sobre peso, piel y cabello. Resultados: ACM/EE fue tomado por 593 mujeres durante 3426 ciclos. Hubo 2 embarazos durante el seguimiento, un...

  3. Estética, política y vida cotidiana. El caso de la pintura callejera chilena

    Directory of Open Access Journals (Sweden)

    Patricio Rodriguez-Plaza

    2005-06-01

    Full Text Available Este artículo trata de compartir una reflexión académica con respecto al fenómeno de la pintura callejera chilena, entendiéndola como un hecho estético total, que lo es menos por sus producciones y resultados que por los usos y percepciones que lo entretejen, conforman y desparraman por la ciudad.

  4. The assembly of papaya mosaic virus coat protein with DNA.

    Science.gov (United States)

    Erickson, J W; Bancroft, J B

    1980-01-01

    Products of specific (pH 8.0-8.5) and nonspecific (pH 6.0) assembly reactions of papaya mosaic virus (PMV) coat protein with DNA are described. The strandedness, topology, and sugar moiety of the nucleic acid are important parameters for assembly in nonspecific conditions. The linear, single-stranded form of lambda DNA, but not the double-stranded form, reacted with PMV protein to form multiply initiated particles whose helical segments apparently annealed to produce continuous tubular particles. With the circular, single-stranded DNA of phi X174, partially tubular, partially extended particles were made. Poly(dA), unlike poly(A) [Erickson JW, AbouHaidar M, Bancroft JB: Virology 90:60, 1978], was not encapsidated by PMV protein under specific assembly conditions. With all DNAs tested, extended particles were the only products formed in specific conditions at pH 8.5.

  5. Identification of gamma-irradiated papaya, melon and watermelon

    Energy Technology Data Exchange (ETDEWEB)

    Marin-Huachaca, N.S.; Mancini-Filho, Jorge E-mail: jmancini@usp.br; Delincee, Henry E-mail: henry.delincee@bfe.uni-karlsruhe.de; Villavicencio, A.L.C.H. E-mail: villavic@net.ipen.br

    2004-10-01

    Ionizing radiation can be used to control spoilage microorganisms and to increase the shelf life of fresh fruits and vegetables in replacement for the treatment with chemical fumigants. In order to enforce labelling regulations, methods for detecting the irradiation treatment directly in the produce are required. Recently, a number of detection methods for irradiated food have been adopted by the Codex Comission. A rapid screening method for qualitative detection of irradiation is the DNA Comet Assay. The applicability of the DNA Comet Assay for distinguishing irradiated papaya, melon, and watermelon was evaluated. The samples were treated in a {sup 60}Co facility at dose levels of 0.0, 0.5, 0.75, and 1.0 kGy. The irradiated samples showed typical DNA fragmentation whereas cells from non-irradiated ones appeared intact. In addition to the DNA Comet Assay also the half-embryo test was applied in melon and watermelon to detect the irradiation treatment.

  6. Identification of gamma-irradiated papaya, melon and watermelon

    Science.gov (United States)

    Marín-Huachaca, Nélida S.; Mancini-Filho, Jorge; Delincée, Henry; Villavicencio, Anna Lúcia C. H.

    2004-09-01

    Ionizing radiation can be used to control spoilage microorganisms and to increase the shelf life of fresh fruits and vegetables in replacement for the treatment with chemical fumigants. In order to enforce labelling regulations, methods for detecting the irradiation treatment directly in the produce are required. Recently, a number of detection methods for irradiated food have been adopted by the Codex Comission. A rapid screening method for qualitative detection of irradiation is the DNA Comet Assay. The applicability of the DNA Comet Assay for distinguishing irradiated papaya, melon, and watermelon was evaluated. The samples were treated in a 60Co facility at dose levels of 0.0, 0.5, 0.75, and 1.0kGy. The irradiated samples showed typical DNA fragmentation whereas cells from non-irradiated ones appeared intact. In addition to the DNA Comet Assay also the half-embryo test was applied in melon and watermelon to detect the irradiation treatment.

  7. Antiulcerogenic activity of Carica papaya seed in rats.

    Science.gov (United States)

    Pinto, Lorraine Aparecida; Cordeiro, Kátia Wolff; Carrasco, Viviane; Carollo, Carlos Alexandre; Cardoso, Cláudia Andréa Lima; Argadoña, Eliana Janet Sanjinez; Freitas, Karine de Cássia

    2015-03-01

    The purpose of the present study was to evaluate the gastroprotective and healing effects of the methanolic extract of the seed of the papaya Carica papaya L. (MECP) in rats. Models of acute gastric ulcer induction by ethanol and indomethacin and of chronic ulcer by acetic acid were used. The gastric juice and mucus parameters were evaluated using the pylorus ligation model, and the involvement of sulfhydryl compounds (GSH) and nitric oxide in the gastroprotective effect was analyzed using the ethanol model. The toxicity was assessed through toxicity tests. No signs of toxicity were observed when the rats received a single dose of 2000 mg/kg of extract. The MECP in doses of 125, 250, and 500 mg/kg significantly reduced the gastric lesion with 56, 76, and 82 % inhibition, respectively, and a dose of 30 mg/kg lansoprazole showed 79 % inhibition in the ethanol model. MECP (125, 250, 500 mg/kg) and cimetidine (200 mg/kg) reduced the gastric lesion in the indomethacin model, with 62, 67, 81, and 85 % inhibition, respectively. The MECP (500 mg/kg) and cimetidine (200 mg/kg) treatments showed a reduction in ulcerative symptoms induced by acetic acid by 84 and 73 %, respectively. The antiulcerogenic activity seems to involve GSH because the inhibition dropped from 72 to 13 % in the presence of a GSH inhibitor. Moreover, the MECP showed systemic action, increasing the mucus production and decreasing gastric acidity. Treatments with MECP induce gastroprotection without signs of toxicity. This effect seems to involve sulfhydryl compounds, increased mucus, and reduced gastric acidity.

  8. Partial rootzone drying (PRD) and regulated deficit irrigation (RDI) effects on stomal conductance, growth, photosynthetic capacity, and water-use efficiency of papaya

    Science.gov (United States)

    Papaya (Carica papaya, L.) is an important economic crop in tropical and subtropical countries in addition to its human health benefits. Papaya is a giant herbaceous species and maintaining adequate tissue turgidity and water availability is necessary to maintain the rigidity of the stem as well as...

  9. El crecimiento de las ciudades chilenas (1865-1982: primacía y dialéctica socioespacial

    Directory of Open Access Journals (Sweden)

    Hilario Hernández Gurruchaga

    2015-04-01

    Full Text Available Este trabajo se inserta en el amplio propósito de investigar la dinámica tempo-espacial del crecimiento de las ciudades chilenas (1865-1982 a la luz de algunos supuestos teóricos y metodológicos actuales, con la intención de describir los procesos de crecimiento; vale decir, las secuencias de cambios estructurales ligados a algún mecanismo explicativo. Específicamente, en una primera aproximación, se describirá el crecimiento de las ciudades chilenas (1865-1982 utilizando como cuerpo heurístico la "norma-rango-tamaño" (Zipf G.K. 1941-1949 y la "ley de efectos proporcionales", (Gibrat para luego buscar una más cabal explicación a través de la historia de la suscesión de modelos político-económicos adoptados por la sociedad chilena, centrando el análisis histórico en los últimos cincuenta años de evolución (1930-82; período central para una real comprensión de los procesos que han conducido a la actual concentración demográfica y económica que caracteriza el país.

  10. Cloning and expression analysis of phytoene desaturase and ζ-carotene desaturase genes in Carica papaya.

    Science.gov (United States)

    Yan, P; Gao, X Z; Shen, W T; Zhou, P

    2011-02-01

    The fruit flesh color of papaya is an important nutritional quality trait and is due to the accumulation of carotenoid. To elucidate the carotenoid biosynthesis pathway in Carica papaya, the phytoene desaturase (PDS) and the ζ-carotene desaturase (ZDS) genes were isolated from papaya (named CpPDS and CpZDS) using the rapid amplification of cDNA ends (RACE) approach, and their expression levels were investigated in red- and yellow-fleshed papaya varieties. CpPDS contains a 1749 bp open reading frame coding for 583 amino acids, while CpZDS contains a 1716 bp open reading frame coding for 572 amino acids. The deduced CpPDS and CpZDS proteins contain a conserved dinucleotide-binding site at the N-terminus and a carotenoid-binding domain at the C-terminus. Papaya genome sequence analysis revealed that CpPDS and CpZDS are single copy; the CpPDS was mapped to papaya chromosome LG6, and the CpZDS was mapped to chromosome LG3. Quantitative PCR showed that both CpPDS and CpZDS were expressed in all tissues examined with the highest expression in maturing fruits, and that the expression of CpPDS and CpZDS were higher in red-fleshed fruits than in yellow-fleshed fruits. These results indicated that the differential accumulation of carotenoids in red- and yellow-fleshed papaya varieties might be partly explained by the transcriptional level of CpPDS and CpZDS.

  11. Antihypertensive effect of Carica papaya via a reduction in ACE activity and improved baroreflex.

    Science.gov (United States)

    Brasil, Girlandia Alexandre; Ronchi, Silas Nascimento; do Nascimento, Andrews Marques; de Lima, Ewelyne Miranda; Romão, Wanderson; da Costa, Helber Barcellos; Scherer, Rodrigo; Ventura, José Aires; Lenz, Dominik; Bissoli, Nazaré Souza; Endringer, Denise Coutinho; de Andrade, Tadeu Uggere

    2014-11-01

    The aims of this study were to evaluate the antihypertensive effects of the standardised methanolic extract of Carica papaya, its angiotensin converting enzyme inhibitory effects in vivo, its effect on the baroreflex and serum angiotensin converting enzyme activity, and its chemical composition. The chemical composition of the methanolic extract of C. papaya was evaluated by liquid chromatography-mass/mass and mass/mass spectrometry. The angiotensin converting enzyme inhibitory effect was evaluated in vivo by Ang I administration. The antihypertensive assay was performed in spontaneously hypertensive rats and Wistar rats that were treated with enalapril (10 mg/kg), the methanolic extract of C. papaya (100 mg/kg; twice a day), or vehicle for 30 days. The baroreflex was evaluated through the use of sodium nitroprusside and phenylephrine. Angiotensin converting enzyme activity was measured by ELISA, and cardiac hypertrophy was evaluated by morphometric analysis. The methanolic extract of C. papaya was standardised in ferulic acid (203.41 ± 0.02 µg/g), caffeic acid (172.60 ± 0.02 µg/g), gallic acid (145.70 ± 0.02 µg/g), and quercetin (47.11 ± 0.03 µg/g). The flavonoids quercetin, rutin, nicotiflorin, clitorin, and manghaslin were identified in a fraction of the extract. The methanolic extract of C. papaya elicited angiotensin converting enzyme inhibitory activity. The antihypertensive effects elicited by the methanolic extract of C. papaya were similar to those of enalapril, and the baroreflex sensitivity was normalised in treated spontaneously hypertensive rats. Plasma angiotensin converting enzyme activity and cardiac hypertrophy were also reduced to levels comparable to the enalapril-treated group. These results may be associated with the chemical composition of the methanolic extract of C. papaya, and are the first step into the development of a new phytotherapic product which could be used in the treatment of hypertension.

  12. A case study to determine the geographical origin of unknown GM papaya in routine food sample analysis, followed by identification of papaya events 16-0-1 and 18-2-4

    NARCIS (Netherlands)

    Prins, Theo W.; Scholtens-Toma, Ingrid; Bak, Arno W.; Dijk, Van Jeroen P.; Voorhuijzen, Marleen M.; Laurensse, Emile J.; Kok, Esther J.

    2016-01-01

    During routine monitoring for GMOs in food in the Netherlands, papaya-containing food supplements were found positive for the genetically modified (GM) elements P-35S and T-nos. The goal of this study was to identify the unknown and EU unauthorised GM papaya event(s). A screening strategy was applie

  13. Use of microsatellite markers in molecular analysis of segregating populations of papaya (Carica papaya L.) derived from backcrossing.

    Science.gov (United States)

    Pinto, F O; Pereira, M G; Luz, L N; Cardozo, D L; Ramos, H C C; Macedo, C M P

    2013-07-08

    Brazil is the world leader in papaya production. However, only a small number of cultivars are registered for commercial planting, mainly owing to delays in obtaining cultivars and the high costs of the field phase of breeding programs. These costs can be reduced when molecular tools are combined with conventional breeding methods. In the present study, we conducted a molecular analysis of a self-fertilized population of a first backcrossing generation of BC1S1 papaya plants via microsatellite markers both to monitor the level of homozygosity and the gene/allele transfer that confers the Golden trait (fruit color) and to assess the parental genomic proportion in the genotypes studied. Based on the analysis of 20 polymorphic microsatellite loci, 19 genotypes with the Golden trait belonging to BC1S1 were evaluated in addition to the parental genotypes. Genetic distance was estimated through weighted index. The genotypes were then grouped using the hierarchical nearest neighbor method, and the analysis of principal coordinates was used to measure the proportion of parental genomes in the segregating genotypes. The mean value of the inbreeding coefficient was 0.36. The analysis of the principal coordinates revealed that on average, 64% of the recurrent parent genome was present in the population. Together, the analyses allowed the selection of 3 individuals for the next backcross cycle (33BC1S1-18, 34BC1S1-16, and 37BC1S1-10). These individuals had a higher proportion of the recurrent parent and were grouped close to the recurrent parent in the cluster analysis.

  14. Label-free quantitative proteomic analysis of pre-flowering PMeV-infected Carica papaya L.

    Science.gov (United States)

    Soares, Eduardo de A; Werth, Emily G; Madroñero, Leidy J; Ventura, José A; Rodrigues, Silas P; Hicks, Leslie M; Fernandes, Patricia M B

    2017-01-16

    Papaya meleira virus (PMeV) infects papaya (Carica papaya L.) and leads to Papaya Sticky Disease (PSD) or "Meleira", characterized by a spontaneous exudation of latex from fruits and leaves only in the post-flowering developmental stage. The latex oxidizes in contact with air and accumulates as a sticky substance on the plant organs, impairing papaya fruit's marketing and exportation. To understand pre-flowering C. papaya resistance to PMeV, an LC-MS/MS-based label-free proteomics approach was used to assess the differential proteome of PMeV-infected pre-flowering C. papaya vs. uninfected (control) plants. In this study, 1333 proteins were identified, of which 111 proteins showed a significant abundance change (57 increased and 54 decreased) and supports the hypothesis of increased photosynthesis and reduction of 26S-proteassoma activity and cell-wall remodeling. All of these results suggest that increased photosynthetic activity has a positive effect on the induction of plant immunity, whereas the reduction of caspase-like activity and the observed changes in the cell-wall associated proteins impairs the full activation of defense response based on hypersensitive response and viral movement obstruction in pre-flowering C. papaya plants.

  15. Influence of coat protein transgene copy number on resistance in transgenic line 63-1 against Papaya ringspot virus isolates

    NARCIS (Netherlands)

    Souza, M.T.; Níckel, O.; Gonsalves, D.

    2005-01-01

    Line 63-1 is a 'Sunset'-derived transgenic papaya expressing the coat protein (CP) gene from a mild mutant of a Hawaiian isolate of Papaya ringspot virus (PRSV). Previous work showed that line 63-1 R, plants exhibited a range of resistance to severe PRSV isolates from Hawaii (HA), Jamaica (JA), Thai

  16. Identification, Expression and IAA-Amide Synthetase Activity Analysis of Gretchen Hagen 3 in Papaya Fruit (Carica papaya L.) during Postharvest Process

    Science.gov (United States)

    Liu, Kaidong; Wang, Jinxiang; Li, Haili; Zhong, Jundi; Feng, Shaoxian; Pan, Yaoliang; Yuan, Changchun

    2016-01-01

    Auxin plays essential roles in plant development. Gretchen Hagen 3 (GH3) genes belong to a major auxin response gene family and GH3 proteins conjugate a range of acylsubstrates to alter the levels of hormones. Currently, the role of GH3 genes in postharvest physiological regulation of ripening and softening processes in papaya fruit is unclear. In this study, we identified seven CpGH3 genes in a papaya genome database. The CpGH3.1a, CpGH3.1b, CpGH3.5, CpGH3.6, and CpGH3.9 proteins were identified as indole-3-acetic acid (IAA)-specific amido synthetases. We analyzed the changes in IAA-amido synthetase activity using aspartate as a substrate for conjugation and found a large increase (over 5-fold) during the postharvest stages. Ascorbic acid (AsA) application can extend the shelf life of papaya fruit. Our data showed that AsA treatment regulates postharvest fruit maturation processes by promoting endogenous IAA levels. Our findings demonstrate the important role of GH3 genes in the regulation of auxin-associated postharvest physiology in papaya.

  17. NIa-Pro of Papaya ringspot virus interacts with Carica papaya eukaryotic translation initiation factor 3 subunit G (CpeIF3G).

    Science.gov (United States)

    Gao, Le; Tuo, Decai; Shen, Wentao; Yan, Pu; Li, Xiaoying; Zhou, Peng

    2015-02-01

    The interaction of papaya eukaryotic translation initiation factor 3 subunit G (CpeIF3G) with Papaya ringspot virus (PRSV) NIa-Pro was validated using a bimolecular fluorescence complementation assay in papaya protoplasts based on the previous yeast two-hybrid assay results. The C-terminal (residues 133-239) fragment of PRSV NIa-Pro and the central domain (residues 59-167) of CpeIF3G were required for effective interaction between NIa-Pro and CpeIF3G as shown by a Sos recruitment yeast two-hybrid system with several deletion mutants of NIa-Pro and CpeIF3G. The central domain of CpeIF3G, which contains a C2HC-type zinc finger motif, is required to bind to other eIFs of the translational machinery. In addition, quantitative real-time reverse transcription PCR assay confirmed that PRSV infection leads to a 2- to 4.5-fold up-regulation of CpeIF3G mRNA in papaya. Plant eIF3G is involved in various stress response by enhancing the translation of resistance-related proteins. It is proposed that the NIa-Pro-CpeIF3G interaction may impair translation preinitiation complex assembly of defense proteins and interfere with host defense.

  18. Molecular diagnosis of Papaya meleira virus (PMeV) from leaf samples of Carica papaya L. using conventional and real-time RT-PCR.

    Science.gov (United States)

    Abreu, Paolla M V; Piccin, João G; Rodrigues, Silas P; Buss, David S; Ventura, José A; Fernandes, Patricia M B

    2012-03-01

    Papaya meleira virus (PMeV) is the causal agent of papaya sticky disease. This study describes two methods for molecular diagnosis of PMeV using conventional and real-time PCR. These methods were shown to be more efficient than current methods of viral detection using extraction of PMeV dsRNA and observation of symptoms in the field. The methods described here were used to evaluate the effect of inoculation of papaya plants with purified PMeV dsRNA on the progress of PMeV infection. A single inoculation with PMeV dsRNA was observed to delay the progress of the virus infection by several weeks. The possibility of vertical transmission of PMeV was also investigated. No evidence was found for PMeV transmission through seeds collected from diseased fruit. The implications of these results for the epidemiology of PMeV and the management of papaya sticky disease are discussed.

  19. Relationships between SAP-flow measurements, whole-canopy transpiration and reference evapotranspiration in field-grown papaya (Carica papaya L.)

    Science.gov (United States)

    Whole-canopy gas exchange measurement in papaya can provide a scientific basis to optimize irrigation, and fruit yield and quality. The objectives of this study were to: 1) verify the relationship between xylem sap flow measured by the heat coefficient method and whole canopy transpiration in ‘Gra...

  20. KURSUS SINGKAT DAN PELATIHAN PEMANFAATAN PEPAYA (CARISA PAPAYA L. MENJADI MANISAN KERING BUAH PEPAYA DI DESA TIMUHUN, KECAMATAN BANJARANGKAN, KABUPATEN KLUNGKUNG

    Directory of Open Access Journals (Sweden)

    I Made Nada

    2012-09-01

    Full Text Available ABSTRACT In Desa Timuhun, eventhough papaya is not primary fruit product, but usually papaya is planted among the other plants and easily to grow. Papaya has good potency to be processed to become food product. Matured papaya fruit is usually fresh served for desk fruit. On the other hand, for papaya which is not mature yet, usually used for vegetable. Papaya has high water content, so it makes papaya fruit easy to experience damage. With papaya processing, papaya which is originally assumed improper to be sold or to be consumed (mechanical damage, diseased, acid, physical damage and will only to be thrown, finally can be used. The making of papaya dry candy is one of effort to preserve and to vary form of papaya serving. It also improves the economic value of papaya. The making of papaya can be conducted in big or small scale. The activity objectives are to introduce and give additional knowledge to society about papaya processing technology, to increase economic added value of papaya and the using of papaya which is improper to be sold or to be consumed becoming valuable food product .Realization of the objectives above are by performing an information sharing and discussion with the society about process of the making dry candy from papaya, training of papaya dry candy making, introduce simple technology of dry candy papaya making. This activity was performed at the head of Desa Timuhun office, on Friday, September 14, 2007 and was attended by 26 persons, including association of woman farmer and the staff in that office. The result of this activity shows that there was positive response of people who attend this activity, because by processing papaya fruit into dry candy, have a lot of advantage compare to sale it freshly. Beside that, this dry candy is healthy snack, without synthetic preservation, enriched with vitamins which is useful for health, also this dry candy is still good up to 1 month and of course it could to improve economic

  1. Carbon disulfide formation in papaya under conditions of dithiocarbamate residue analysis.

    Science.gov (United States)

    Abakerli, R B; Sparrapan, R; Sawaya, A C H F; Eberlin, M N; Jara, J L P; Rodrigues, N R; Fay, E F; Luiz, A J B; Galvão, T D L; Martins, D dos S; Yamanishi, O K; Toledo, H H B

    2015-12-01

    Golden, Sunrise Solo and Tainung cultivars of papaya were found to release CS2 when submitted to experimental conditions of dithiocarbamate residue analysis. Three common analytical methods were used to quantitate CS2; one spectrophotometric method and two chromatographic methods. All three methods gave positive CS2 results for all three papaya varieties. Other endogenous compounds present in isooctane extracts of papaya fractions detected via gas chromatography (GC/ITD) using electron ionization (EI) were: carbonyl sulfide, dimethyl sulfide, carbon disulfide, 2-methylthiophene, 3-methylthiophene, 2-ethylthiophene, 3-ethylthiophene, benzylisothiocyanate, benzylthiocyanate and benzonitrile. Control samples were obtained from papaya plantations cultivated in experimental areas, in which no treatment with fungicides of the dithiocarbamate group was applied. Endogenous CS2 levels were compared with true dithiocarbamate residues measured in papaya samples from the field trials following applications of the mancozeb fungicide. Three days after application, true dithiocarbamate residues, measured by the procedure with isooctane partitioning and GC-ITD, were at the average level of 2 mg kg(-1).

  2. Study on shelf life extension of papayas irradiated by /sup 60/Co gamma-rays

    Energy Technology Data Exchange (ETDEWEB)

    Chang, M.S.; Chen, M.D.; Lin, C.T.; Fu, Y.K.

    1984-11-01

    Papayas are one of the main fresh fruits in Taiwan area. Papaya fruits were treated with hot water to pasteurize peels followed by /sup 60/Co gamma-ray irradiation to extend the ripening time. The purpose of synergetic methods is to extend the shelf life of papaya fruits. This experiment was carried out by seven treatments, which were: (1) control group, (2) hot water treatment only, (3) hot water treatment with a 25 krad ..gamma..-irradiation, (4) hot water treatment with a 50 krad ..gamma..-irradiation, (5) hot water treatment with a 75 krad ..gamma..-irradiation, (6) 75 krad ..gamma..-irradiation only, and (7) hot water treatment with a 100 krad ..gamma..-irradiation. The items of observation were: surface yellowing, surface decaying, quality of texture, and length of period lasted after irradiation for 50% marketable papayas. The results of this study showed that a shelf-life extension of six days could be obtained for papayas subjected to hot water (50 to 55/sup 0/C) treatment and a 100 krad irradiation. 3 refs., 2 figs., 2 tabs.

  3. Data on screening and identification of genetically modified papaya in food supplements.

    Science.gov (United States)

    Prins, Theo W; Scholtens, Ingrid M J; Bak, Arno W; van Dijk, Jeroen P; Voorhuijzen, Marleen M; Laurensse, Emile J; Kok, Esther J

    2016-12-01

    This article contains data related to the research article entitled "A case study to determine the geographical origin of unknown GM papaya in routine food sample analysis, followed by identification of papaya events 16-0-1 and 18-2-4" (Prins et al., 2016) [1]. Quantitative real-time PCR (qPCR) with targets that are putatively present in genetically modified (GM) papaya was used as a first screening to narrow down the vast array of candidates. The combination of elements P-nos and nptII was further confirmed by amplification and subsequent sequencing of the P-nos/nptII construct. Next, presence of the candidate GM papayas 16-0-1 and 18-2-4 were investigated by amplification and sequencing of event-spanning regions on the left and right border. This data article reports the Cq values for GM elements, the nucleotide sequence of the P-nos/nptII construct and the presence of GM papaya events 18-2-4 and/or 16-0-1 in five samples that were randomly sampled to be analysed in the framework of the official Dutch GMO monitoring program for food.

  4. Cloning and characterization of a FLORICAULA/LEAFY ortholog, PFL, in polygamous papaya

    Institute of Scientific and Technical Information of China (English)

    Qingyi YU; Paul H. MOORE; Henrik H. ALBERT; Adrienne H.K. ROADER; Ray MING

    2005-01-01

    The homologous genes FLORICAULA (FLO) in Antirrhinum and LEAFY (LFY) in Arabidopsis are known to regulate the initiation of flowering in these two distantly related plant species. These genes are necessary also for the expression of downstream genes that control floral organ identity. We used Arabidopsis LFY cDNA as a probe to clone and sequence a papaya ortholog of LFY, PFL. It encodes a protein that shares 61% identity with the Arabidopsis LFY gene and 71% identity with the LFYhomologs of the two woody tree species: California sycamore (Platanus racemosa) and black cottonwood (Populus trichocarpa). Despite the high sequence similarity within two conserved regions, the N-terminal proline-rich motif in papaya PFL differs from other members in the family. This difference may not affect the gene function of papaya PFL, since an equally divergent but a functional LFY ortholog NEEDLY of Pinus radiata has been reported. Genomic and BAC Southern analyses indicated that there is only one copy of PFL in the papaya genome. In situ hybridization experiments demonstrated that PFL is expressed at a relatively low level in leaf primordia,but it is expressed at a high level in the floral meristem. Quantitative PCR analyses revealed that PFL was expressed in flower buds of all three sex types - male, female, and hermaphrodite with marginal difference between hermaphrodite and unisexual flowers. These data suggest that PFL may play a similar role as LFY in flower development and has limited effect on sex differentiation in papaya.

  5. Verbo de concordancia en la lengua de señas chilena

    Directory of Open Access Journals (Sweden)

    Dora Adamo Quintela

    1999-09-01

    Full Text Available El presente trabajo trata sobre una clase verbo de la lengua de señas chilena, el verbo de concordancia. A través de su descripción se da cuenta de los diversos mecanismos utilizados por el sistema de la lengua de señas para establecer la concordancia entre el sujeto y el objeto, que funcionan en la oración mediante el proceso de inflexión gramatical. Una característica relevante de la LSCh consiste en la utilización del espacio para producir modificaciones gramaticales regulares, entendidas como procesos de inflexión, a través de la co-ocurrencia de parámetros gestual-visuales, que permiten una comunicación eficiente entre sus usuarios. (This article focuses on a particular type of verb used in Chilean Sign Language: the verb of agreement. Through its description, different mechanisms used to show subject-object accord operating through inflection are presented. A relevant feature of Chilean Sign Language (LSCh, is to use space to mark regular grammatical modifications, understood as processes of inflection through the co-occurrence of visual-kinetic parameters that allow its user an efficient communication.

  6. Equilibrio trabajo-familia: corresponsabilidad familiar y autoeficacia parental en trabajadores de una empresa chilena

    Directory of Open Access Journals (Sweden)

    Alejandra Ortega Farías

    2013-01-01

    Full Text Available En el artículo se analizan las diferencias entre equilibrio trabajo-familia, corresponsabilidad y autoeficacia parental en trabajadores de una empresa chilena. A 224 trabajadores les fueron aplicados tres instrumentos: el cuestionario de interacción trabajofamilia, el cuestionario de participación en el trabajo familiar y la escala de autoeficacia parental. Se observa que no existe diferencia en el nivel de equilibrio trabajo-familia entre hombres y mujeres (t [222] = -0,83, p > 0,05. En corresponsabilidad se evidencian diferencias estadísticamente significativas (t [220,28] = -7,27, p < 0,05, indicando que las mujeres realizan más tareas en el hogar, a pesar de su jornada laboral. En relación con la autoeficacia parental existen diferencias entre hombres y mujeres (t [215] = -8,76, p <0,05, indicando que las mujeres se perciben a sí mismas como mejores madres.

  7. Política educativa chilena 1965-2009. ¿Qué oculta esa trama?

    Directory of Open Access Journals (Sweden)

    María Angélica Oliva

    2010-01-01

    Full Text Available Se aborda la política educativa chilena del período 1965-2009, inquiriéndose, ¿Qué oculta la trama? La conjetura es, la política educativa, a excepción del gobierno de Allende, muestra la institución del orden neoliberal. Se revisan algunas antinomias, mecanismos de ese orden, que se introduce en1965 con la pedagogía por objetivos y la teoría del capital humano. Esta pedagogía, es profundizada en dictadura, junto a la idea de subsidiariedad, que impulsa el cambio del rol del Estado, regulado en la Constitución de 1980, aún vigente, y presente en la Ley General de Educación. La municipalización, la transformación en el financiamiento y la pérdida del estatus de funcionario público del profesorado, son un legado de la dictadura. Ello, desperfila el afán democrático de la actual política educativa, transformándola en cómplice de un sistema que genera desigualdad y fragmentación social.

  8. LOS DERECHOS SOCIALES DE PRESTACIÓN EN LA JURISPRUDENCIA CHILENA

    Directory of Open Access Journals (Sweden)

    José Ignacio Martínez Estay

    2010-01-01

    Full Text Available Parte importante de los derechos sociales consisten en prestaciones. Desde el punto de vista jurídico ello parece ser una limitante para su garantía y satisfacción, pues dependen de los recursos económicos de que disponga el Estado. Por eso, lo usual es que si se constitucionalizan no llevan aparejada una garantía jurisdiccional. Así ocurre en la Constitución chilena. Dicha limitación se ve ratificada por la jurisprudencia de la Corte Suprema, que reiteradamente ha negado lugar a recursos de protección en que se intentaba la tutela de derechos sociales consistentes en prestaciones, vinculándolos a algún derecho protegido jurisdiccionalmente. Pero la reciente jurisprudencia del Tribunal Constitucional respecto del derecho a la protección de la salud y del derecho a la seguridad social contradice lo anterior, atribuyéndole a los derechos sociales consistentes en prestaciones el mismo valor que el de los demás derechos y libertades.

  9. FACTORES DETERMINANTES DEL ENDEUDAMIENTO BANCARIO EN LA EMPRESA NO FINANCIERA CHILENA

    Directory of Open Access Journals (Sweden)

    Mauricio Jara Bertin

    2012-01-01

    Full Text Available El objetivo de este artículo es constatar si la elección de recurrir a la banca como fuente de financiación viene determinada por las oportunidades de crecimiento, ta-maño de la empresa, calidad de los proyectos, la calidad de las garantías prendarias y el riesgo de la empresa. Hemos utilizado una muestra de 168 empresas chilenas cotizadas en la Bolsa durante el periodo 1997-2008, que nos ha servido para exami-nar la relación entre factores específicos de las empresas y el endeudamiento ban-cario. Nuestros resultados evidencian que las oportunidades de crecimiento tienen una influencia positiva en el endeudamiento bancario, que se torna negativa ante la existencia de empresas de mayor tamaño con oportunidades de inversión. Ade-más, el tamaño de la empresa y la calidad de los proyectos en curso se relacionan negativamente, mientras que la calidad de las garantías prendarias y el riesgo de la empresa tienen una incidencia positiva en el endeudamiento bancario.

  10. Nuestro espejo de cartón: educación y posdictadura chilena

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    Pamela Tamara Salazar Vicencio

    2011-05-01

    Full Text Available Nos interesa definir cómo se constituye la propuesta educacional en torno a la problemática de la memoria y los derechos humanos en Chile, a partir del análisis del discurso articulado desde el Museo de la Memoria y los Derechos Humanos, obra cúlmine del último gobierno de la Concertación y versión oficial en torno a la memoria del pasado reciente en el país. Para estos efectos, revisaremos ciertos aspectos del espacio físico en donde se edifica el museo, leyendo los lugares a la luz de nociones teóricas en torno a la museificación y estetización de la memoria; sin embargo, el eje de la investigación consiste en la lectura crítica del material que se ofrece como alternativa a los docentes que preparan a sus alumnos para la visita guiada.La hipótesis tentativa es que existe una visión estatal que victimiza a los sujetos afectados directamente, limitando el espectro de impacto a un grupo minoritario de la población chilena; que igualmente apunta a despolitizar el conflicto mediante la instalación de una memoria parcial, estática y lineal en torno al pasado y que cristaliza parte de sus objetivos en una propuesta educacional ideologizante, que aborda consecuencias sin analizar procesos ni actores sociales.

  11. Stability and efficiency of dye-sensitized solar cells based on papaya-leaf dye

    Science.gov (United States)

    Suyitno, Suyitno; Saputra, Trisma Jaya; Supriyanto, Agus; Arifin, Zainal

    2015-09-01

    The present article reports on the enhancement of the performance and stability of natural dye-based dye-sensitized solar cells (DSSCs). Natural dyes extracted from papaya leaves (PL) were investigated as sensitizers in TiO2-based DSSCs and evaluated in comparison with N719 dye. The acidity of the papaya-leaf extract dyes was tuned by adding benzoic acid. The TiO2 film-coated fluorine-doped tin oxide glass substrates were prepared using the doctor-blade method, followed by sintering at 450 °C. The counter electrode was coated by chemically deposited catalytic platinum. The working electrodes were immersed in N719 dye and papaya dye solutions with concentrations of 8 g/100 mL. The absorbance spectra of the dyes were obtained by ultra-violet-visible spectroscopy. The energy levels of the dyes were measured by the method of cyclic voltammetry. In addition, Fourier transform infrared spectroscopy was used to determine the characteristic functionalities of the dye molecules. The DSSC based on the N719 dye displayed a highest efficiency of 0.87% whereas those based on papaya-leaf dye achieved 0.28% at pH 3.5. The observed improved efficiency of the latter was attributed to the increased current density value. Furthermore, the DSSCs based on papaya-leaf dye with pH 3.5-4 exhibited better stability than those based on N719 dye. However, further studies are required to improve the current density and stability of natural dye-based DSSCs, including the investigation of alternative dye extraction routes, such as isolating the pure chlorophyll from papaya leaves and stabilizing it.

  12. Habitat fragmentation threatens wild populations of Carica papaya (Caricaceae) in a lowland rainforest.

    Science.gov (United States)

    Chávez-Pesqueira, Mariana; Suárez-Montes, Pilar; Castillo, Guillermo; Núñez-Farfán, Juan

    2014-07-11

    • Premise of the study: Wild populations of domesticated species constitute a genetic reservoir and are fundamental to the evolutionary potential of species. Wild papaya (Carica papaya) is a rare, short-lived, gap-colonizing, dioecious tree that persists in the forest by continuous dispersal. Theoretically, these life-history characteristics render wild papaya highly susceptible to habitat fragmentation, with anticipated negative effects on its gene pool. Further, species dioecy may cause founder effects to generate local biases in sex ratio, decreasing effective population size.• Methods: We contrasted the genetic diversity and structure of C. papaya between wild populations from rainforest fragments and continuous forest at Los Tuxtlas, Mexico. We evaluated recent migration rates among populations as well as landscape resistance to gene flow. Finally, we calculated the sex ratio of the populations in both habitats.• Key results: Populations of wild papaya in rainforest fragments showed lower genetic diversity and higher population differentiation than populations in continuous rainforest. Estimates of recent migration rates showed a higher percentage of migrants moving from the continuous forest to the forest fragments than in the opposite direction. Agricultural land and cattle pasture were found to be the most resistant matrices to gene flow. Finally, biased sex ratios were seen to affect the effective population size in both habitats.• Conclusions: The mating system, rarity, and short life cycle of C. papaya are exacerbating the effects of rainforest fragmentation on its genetic diversity, threatening the persistence of its natural populations in the proposed place of origin as well as its genetic reservoir.

  13. Scarabaeidae family species in the Carica papaya L. in Ciego de Ávila

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    Maria Luisa Sisne Luis

    2016-03-01

    Full Text Available A white light trap was placed in Carica papaya L. plantations, as Sisne, 2009 and MINAG, 1985 establishes, in the Citric Enterprise of Ciego de Ávila during the period between º May and July of 2010 with the objective of determining the composition of genus and species of the order Coleoptera family Scarabaeidae associated to the agroecosystem. The species. Cyclocephala cubana Chapin, Anomala calceata Chev. y Phyllophaga crenaticollis Blanch are associated to Carica papaya L. crops in these areas.

  14. Bioactivity of Carica papaya (Caricaceae against Spodoptera frugiperda (Lepidoptera: Noctuidae

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    Norma Cecilia Cárdenas-Ortega

    2011-09-01

    Full Text Available The composition of a chloroform seed extract of C. papaya was determined by GC-MS. Nineteen compounds were identified, with oleic (45.97%, palmitic (24.1% and stearic (8.52% acids being the main components. The insecticidal and insectistatic activities of the extract and the three main constituents were tested. Larval duration increased by 3.4 d and 2.5 d when the extract was used at 16,000 and 9,600 ppm, respectively, whereas the pupal period increased by 2.2 d and 1.1 d at the same concentrations. Larval viability values were 0%, 29.2%, and 50% when the extract was applied at 24,000, 16,000, and 9,600 ppm, respectively; pupal viability was 42.9% and 66.7% at 16,000 and 9,600 ppm; and pupal weight decreased by 25.4% and 11.5% at 16,000 and 9,600 ppm. The larval viability of the main compounds was 33.3%, 48.5%, and 62.5% when exposed to 1,600 ppm of palmitic acid, oleic acid, or stearic acid, respectively.

  15. La Excepción Chilena y las Percepciones de Género en la Participación Laboral Femenina

    OpenAIRE

    Dante Contreras; Agustin Hurtado; M. Francisca Sara

    2012-01-01

    Las mujeres chilenas exhiben una de las tasas de participación laboral más bajas de Latinoamérica, a pesar del crecimiento económico de las últimas décadas y de tener el mayor nivel de escolaridad de la región después de las mujeres cubanas. Este fenómeno es conocido como la Excepción Chilena, y a pesar de su impacto directo en el crecimiento de largo plazo y en los índices de pobreza y desigualdad, existen pocos trabajos que la aborden directamente. En general, la literatura para Chile ha us...

  16. Label-free quantitative proteomics reveals differentially regulated proteins in the latex of sticky diseased Carica papaya L. plants.

    Science.gov (United States)

    Rodrigues, Silas P; Ventura, José A; Aguilar, Clemente; Nakayasu, Ernesto S; Choi, HyungWon; Sobreira, Tiago J P; Nohara, Lilian L; Wermelinger, Luciana S; Almeida, Igor C; Zingali, Russolina B; Fernandes, Patricia M B

    2012-06-18

    Papaya meleira virus (PMeV) is so far the only described laticifer-infecting virus, the causal agent of papaya (Carica papaya L.) sticky disease. The effects of PMeV on the laticifers' regulatory network were addressed here through the proteomic analysis of papaya latex. Using both 1-DE- and 1D-LC-ESI-MS/MS, 160 unique papaya latex proteins were identified, representing 122 new proteins in the latex of this plant. Quantitative analysis by normalized spectral counting revealed 10 down-regulated proteins in the latex of diseased plants, 9 cysteine proteases (chymopapain) and 1 latex serine proteinase inhibitor. A repression of papaya latex proteolytic activity during PMeV infection was hypothesized. This was further confirmed by enzymatic assays that showed a reduction of cysteine-protease-associated proteolytic activity in the diseased papaya latex. These findings are discussed in the context of plant responses against pathogens and may greatly contribute to understand the roles of laticifers in plant stress responses.

  17. BARRERAS DE APRENDIZAJE PARA ESTUDIANTES CON DISCAPACIDAD EN UNA UNIVERSIDAD CHILENA. DEMANDAS ESTUDIANTILES – DESAFÍOS INSTITUCIONALES

    OpenAIRE

    Angélica Corrales Huenul; Valentina Soto Hernández; Gabriela Villafañe Hormazábal

    2016-01-01

    El presente artículo expone los resultados de una investigación sobre estudiantes con discapacidad de una universidad chilena. El objetivo del estudio es determinar las barreras para el aprendizaje y la participación que enfrentan estos estudiantes y, de esta manera, determinar los apoyos educativos requeridos para que estos puedan realizar sus estudios de manera equitativa en relación con la población estudiantil universitaria. En el plano metodológico, la investigación fue definida como des...

  18. Barreras de aprendizaje para estudiantes con discapacidad en una universidad chilena. Demandas estudiantiles y desafíos institucionales

    OpenAIRE

    Corrales Huenul, Angélica; Soto Hernández, Valentina; Villafañe Hormazábal, Gabriela

    2016-01-01

    El presente artículo expone los resultados de una investigación sobre estudiantes con discapacidad de una universidad chilena. El objetivo del estudio es determinar las barreras para el aprendizaje y la participación que enfrentan estos estudiantes y, de esta manera, determinar los apoyos educativos requeridos para que estos puedan realizar sus estudios de manera equitativa en relación con la población estudiantil universitaria. En el plano metodológico, la investigación fue definida como des...

  19. Interculturalidad y ciencias de la educación en lengua de señas chilena

    OpenAIRE

    Carolina-Alejandra-de-Lourdes Becerra-Sepúlveda

    2013-01-01

    Los paradigmas de intervención orales para la cultura sorda afectan la percepción sobre la lengua de señas Chilena . Esto provoca debates emanados de una subvaloración, dadas sus particularidades visuales: iconicidad e isomorfismo, subvaloración nacida de una incomprensión en la lectura de investigaciones internacionales (por escasez de estudios nacionales), especialmente acerca del lenguaje corporeizado. Esto se contradice con nuevas investigaciones en psicolingüística cognitiva acer-ca de e...

  20. DISCURSO ACADÉMICO DE LANZAMIENTO DE LA REVISTA CHILENA DE DERECHO Y CIENCIA POLÍTICA

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    Patricio - Ignacio Carvajal

    2012-07-01

    Full Text Available Me complace enormemente la invitación que se me ha cursado para dirigir algunaspalabras en el lanzamiento de esta Revista Chilena de Derecho y Ciencia Política.Me honra el hecho de que me hayan tenido Uds. en consideración para este actoacadémico y, por supuesto, también que me hayan incorporado al elenco de especialistasde su Comité Científi co Externo. Por ello, comienzo por expresar mi sinceroagradecimiento.

  1. De Indios “Bárbaros” a Vasallos en la Frontera Meridional Chilena Durante el Reformismo Borbónico.

    OpenAIRE

    Chauca García, Jorge

    2017-01-01

    Con la presente Tesis Doctoral abordamos críticamente la visión ilustrada del indígena de la frontera meridional chilena como marco geográfico y la centuria de la Ilustración como coordenada temporal, especialmente su segunda mitad. Un análisis de sus tres familias básicas que no contempla por razones cronológicas a los picunches o gentes del norte, pero sí a los mapuches –gente de la tierra–, huilliches –gente...

  2. La construcción discursiva de los imaginarios sociales: el caso de la medicina popular chilena

    OpenAIRE

    Gerardo Álvarez; Andrea Álvarez; Marisol Facuse

    2002-01-01

    En este artículo se da cuenta del estado de avance de una investigación interdisciplinaria (lingüística, psicología, sociología) sobre las representaciones de cuerpo (salud/enfermedad) en la medicina popular chilena (Proyecto FONDECYT 1000376). No se trata de una investigación sobre la medicina como tal. El objetivo es rastrear el imaginario sobre el cuerpo que subyace a las prácticas curativas y que se revela a través del discurso de las sanadoras populares (mapuches y no mapuches), y luego ...

  3. IDENTIFICACIÓN MEDIANTE PCR DEL SEXO DE LA PAPAYA (Carica papaya L., HÍBRIDO "POCOCÍ"

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    Ericka Saalau-Rojas

    2009-01-01

    con dos metodologías de extracción, CTAB y lisis alcalina (NaOH. La amplificación por PCR del ADN extraído de muestras foliares de papaya híbrido "Pococí", con ambos métodos de extracción, produjo los fragmentos del tamaño esperado. La determinación del sexo de 1.500 plántulas en almácigo mostró un 46 % de plántulas hermafroditas y un 54 % de plantas femeninas. La proporción observada de plantas femeninas: hemafroditas no varió de la esperada (1:1 según la prueba de chi-cuadrado (p= 0,4237. Las plantas hermafroditas fueron llevadas al campo y al momento de la floración se determinó su sexo. La correspondencia entre el sexado por PCR y la expresión sexual en campo fue de un 98 %.

  4. Purification and characterization of a papaya (Carica papaya L.) pectin methylesterase isolated from a commercial papain preparation.

    Science.gov (United States)

    Vasu, Prasanna; Savary, Brett J; Cameron, Randall G

    2012-07-15

    We purified a Carica papaya pectin methylesterase (CpL-PME; EC 3.1.1.11) from a commercial papain preparation. This CpL-PME was separated from the abundant cysteine endopeptidases activities using sequential hydrophobic interaction and cation-exchange chromatographies and then purified by affinity chromatography using Sepharose-immobilized kiwi PME inhibitor protein to obtain a single electrophoretically homogeneous protein. The enzyme was purified 92-fold with 38% yield, providing a specific activity of 1200 U/mg. The molecular weight was determined to be 35,135 by MALDI-TOF-MS in linear mode. MALDI-TOF-MS peptide mass fingerprinting following trypsin digestion indicated CpL-PME represents a novel Carica PME isoform. The CpL-PME required salt for activity, and it showed a broad activity range (pH 6-9) and moderate thermostability (optimum ca. 70°C). A calcium-insensitive methylated lime pectin treated with CpL-PME to reduce degree of methylesterification by 6% converted the substrate to high calcium sensitivity, indicating a processive mode of action. These properties support further research to apply CpL-PME to tailor pectin nanostructure.

  5. Anglicismos y aculturación en la sociedad chilena (English loanwords: acculturation in chilean society

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    Constanza Gerding Salas

    2012-06-01

    Full Text Available La implementación del modelo económico neoliberal estadounidense enChile hace cuatro décadas y la participación del país en la globalización han provocado cambios fundamentales en la sociedad. De hecho, los efectos de la adopción de dicho paradigma se reflejan en diversos ámbitos, incluido el del uso de la lengua. Por esta razón, resulta de interés para lingüistas y traductores indagar acerca de la influencia del idioma inglés y de la cultura estadounidense en la sociedad chilena. De allí que los objetivos de este estudio fueron constatar la presencia de préstamos del inglés en la prensa escrita, para describirlos, determinar su frecuencia de uso e inferir las causas de su adopción. Para lograr estos objetivos se clasificaron de forma manual y semiautomática préstamos del inglés extraídos de periódicos entre enero de 2003 y diciembre de 2009. Se analizó las unidades recopiladas según sus características formales, frecuencia de aparición y áreas del saber a las que pertenecían. Se determinó que los préstamos del inglés se adoptan más que adaptan y que tienen una fuerte presencia en la redacción periodística actual, específicamente en economía, computación, deportes y cultura. Además, se pudo establecer que su uso estaría determinado por cuatro factores: vacío denominativo, prestigio social, economía lingüística y preferencia de los usuarios. Finalmente, se concluyó que la lengua inglesa y la cultura estadounidense tienen una presencia significativa en la prensa chilena. (The implementation of the US-inspired neoliberal economic model in Chile four decades ago and the country’s participation in the globalization process have led to fundamental societal changes. In fact, the effect of the adoption of this paradigm is reflected in several areas, including that of the use of the language. For this reason, it is interesting for linguists and translators to find out the influence of the English language and

  6. Antihyperglycaemic effects of ethanol extracts of Carica papaya and Pandanus amaryfollius leaf in streptozotocin-induced diabetic mice.

    Science.gov (United States)

    Sasidharan, Sreenivasan; Sumathi, Vello; Jegathambigai, Naidu Rameshwar; Latha, Lachimanan Yoga

    2011-12-01

    Diabetes mellitus is a global disease that is increasing in an alarming rate. The present study was undertaken to study the antidiabetic effect of the ethanol extracts of Carica papaya and Pandanus amaryfollius on streptozotocin-induced diabetic mice. The results of the present study indicated that there was no significant difference in the body weight of the treated groups when compared to diabetic control. Whereas, there was significant (P papaya and P. amaryfollius. The antidiabetic effect of C. papaya and P. amaryfollius observed in the present study may be due to the presence of these phytochemicals.

  7. INCORPORACIÓN DEL PAISAJE EN LA GESTIÓN DEL TERRITORIO DE LA PATAGONIA CHILENA

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    MARÍA DOLORES MUÑOZ

    2014-01-01

    Full Text Available Este artículo presenta una metodología para el análisis y gestión del paisaje en la Patagonia chilena, un territorio que se destaca por la belleza y diversidad de los paisajes del agua generados por la presencia de grandes lagos, ríos, fiordos, humedales y campos de hielo. El primer caso de estudio es una investigación para evaluar las potencialidades turísticas y territoriales de los paisajes del agua en la cuenca del río Baker. Se utiliza una metodología interdisciplinaria y técnicas de medición, cualitativa y cuantitativa, que permitieran analizar los valores espaciales, ambientales, territoriales y culturales asociados al paisaje. Un aspecto esencial fue incorporar la valoración social del paisaje. El segundo caso, ubicado en el estrecho de Magallanes, corresponde a una metodología de análisis territorial como base para diseñar un Plan de Manejo Turístico en el Área Marina Protegida Francisco Coloane (que incluye al Parque Marino Francisco Coloane. Comprende el análisis de las condiciones ambientales, las características del paisaje y potencialidades del turismo de intereses especiales asociado al paisaje para respaldar la propuesta de gestión. En ambos casos, la metodología considera al paisaje como principal recurso territorial y plantea una forma de gestión respaldada por principios de sustentabilidad ambiental y social.

  8. Interacciones tempranas y género infantil en familias monoparentales chilenas

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    Marcia Olhaberry Huber

    2012-01-01

    Full Text Available Se realizó un estudio transversal de las interacciones madre-hijo(a en familias monoparentales chilenas de bajo ingresos, considerando las diferencias según el género infantil. Participan 80 díadas, con 39 niñas y 41 niños entre los 4 y 15 meses y se evalúa la calidad de las interacciones con el instrumento CARE-Index. Se observan altos puntajes en depresión y estrés materno, así como un promedio en la calidad de la interacción que indica necesidad de intervención, mostrando lo anterior vulnerabilidad en las díadas del estudio. Las díadas con infantes de género masculino muestran interacciones significativamente más difíciles con sus madres que las de género femenino, quienes a su vez muestran interacciones significativamente más pasivas que los infantes de género masculino. Los tamaños de efecto observados en las diferencias son medianos en ambos casos. Se observa también complementariedad en las conductas de madres e hijos(as, obteniéndose correlaciones positivas y significativas entre sensibilidad materna y cooperatividad infantil, control materno y las conductas difíciles de los infantes, así como entre la conducta no responsiva de las madres y la actitud pasiva en los niños(as. Se discuten los hallazgos a partir de las teorías existentes en relación a género infantil e interacciones tempranas, en el contexto de familias monoparentales a cargo de la madre.

  9. Valorization of agroindustrial wastes: Identification by LC-MS and NMR of benzylglucosinolate from papaya (Carica papaya L.) seeds, a protective agent against lipid oxidation in edible oils.

    Science.gov (United States)

    Castro-Vargas, Henry I; Baumann, Wolfram; Parada-Alfonso, Fabián

    2016-07-01

    In the present study we report the characterization of benzylglucosinolate (BG) isolated from papaya (Carica papaya L.) seeds. A methanolic extract was fractionated and further purified by solid phase extraction (SPE). It was analyzed by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS), and nuclear magnetic resonance spectroscopy ((1) H and (13) C-NMR) as well, and the target compound BG was identified by these two techniques. The effect of BG on lipid oxidation in edible vegetable oil (EO) was shown by observing some lipid oxidation products (linoleic acid hydroperoxides, LHP; hexanal, HEX; nonanal, NON; thiobarbituric acid reactives species, TBARS). BG reduced lipid oxidation production in EO by over 80%, as compared to a control sample and in this way has proved to be a useful antioxidant, even more effective than some antioxidants used by food industry.

  10. Caracterización de accesiones de papaya (Carica papaya L. a través de marcadores AFLP en Cuba

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    Maruchi Alonso Esquivel

    2010-01-01

    Full Text Available Los marcadores moleculares son herramientas valiosas en los estudios genéticos en plantas, y están siendo empleados exitosamente en programas de mejoramiento principalmente en la elección de progenitores y en la selección. El polimorfismo observado mediante la técnica molecular AFLP (Amplified Fragment Length Polymorphism ha sido de utilidad para estudios de diversidad genética en frutales. En el presente trabajo se realizó la caracterización molecular de 12 accesiones de papaya (Carica papaya L. del banco de germoplasma del Instituto de Investigaciones en Fruticultura Tropical (IIFT, empleando la técnica AFLP. Se evaluaron seis combinaciones de iniciadores para la amplificación selectiva, las cuales amplificaron un total de 431 bandas con 73,3% de polimorfismo. El número total de patrones de bandas identificados fue igual en todas las combinaciones utilizadas, con un porcentaje de identificación alto, lo que sugiere que dichas combinaciones pudieran ser empleadas para estudios de variabilidad genética en papaya. En general, los resultados presentados demuestran que existe diversidad genética entre las accesiones evaluadas, lo cual constituye un reflejo del origen que presentan los genotipos analizados a partir de la introducción de materiales foráneos y la polinización abierta de un grupo de materiales selectos. Por tanto, se recomienda retomar la prospección y selección de accesiones locales, así como la introducción de nuevos genotipos foráneos, como dos vías fundamentales para aumentar la diversidad genética presente en el banco de germoplasma de papaya de Cuba. Palabras clave: marcadores moleculares, polimorfismo, diversidad genética.

  11. Potencial antifúngico de extractos de cuatro especies vegetales sobre el crecimiento de Colletotrichum gloeosporioides en papaya (Carica papaya en poscosecha

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    Nadia Landero Valenzuela

    2013-01-01

    Full Text Available El manejo de antracnosis por Colletotrichum gloeosporioides es el problema más importante en poscosecha de frutos tropicales. La actividad antifúngica de diferentes extractos vegetales fue evaluada in vitro e in vivo para controlar la antracnosis poscosecha en papaya. Extractos de ajo (Allium sativum (10 y 15 % y canela (Cinnamomum zeylanicum (0,0050; 0,0100; 0,0150 % mostraron efecto fungicida en contra de C. gloeosporioides para supresión de crecimiento micelial (100 %, inhibición de germinación (100 % y esporulación del hongo (100 %, considerándose estos extractos como los más promisorios para inhibir el desarrollo del hongo in vitro. Estudios in vivo también revelaron que los extracto de ajo (11,74 % y canela a dosis de 0,0054 % aplicada antes y al mismo tiempo de la inoculación con C. gloeosporioides, fueron las dosis óptimas para el control (severidad de la antracnosis en frutos de papaya artificialmente inoculados, mientras que a mayores dosis la severidad aumentó. La variable fitotoxicidad arrojó resultados consistentes con lo anterior, comprobándose que al aumentar las dosis de extracto de canela se presentó cambio de color en los frutos. Los resultados sugieren la posibilidad de usar estos extractos a dosis adecuadas como biofungicidas para controlar antracnosis en papaya en poscosecha.

  12. Resistance of non-transgenic papaya plants to papaya ringspot virus (PRSV) mediated by intron-containing hairpin dsRNAs expressed in bacteria.

    Science.gov (United States)

    Shen, W; Yang, G; Chen, Y; Yan, P; Tuo, D; Li, X; Zhou, P

    2014-01-01

    RNA-mediated virus resistance based on natural antiviral RNA silencing has been exploited as a powerful tool for engineering virus resistance in plants. In this study, a conserved 3'-region (positions 9839-10117, 279 nt) of the capsid protein (CP) gene of papaya ringspot virus (PRSV), designated CP279, was used to generate an intron-containing hairpin RNA (ihpRNA) construct by one-step, zero-background ligation-independent cloning (OZ-LIC). The RNaseIII-deficient Escherichia coli strain M-JM109lacY was identified as the best choice for producing large quantities of specific ihpRNA-CP279. Resistance analyses and ELISA data verified that most papaya plants mechanically co-inoculated with TRIzol-extracted ihpRNA-CP279 and PRSV were resistant to PRSV, and resistance was maintained throughout the test period (>2 months post-inoculation). In contrast, a 1-2 day interval between sequential inoculation of PRSV and ihpRNA-CP279 did not result in complete protection against PRSV infection, but delayed the appearance of viral symptoms by 3 to 4 days. These findings indicate that direct mechanical inoculation of papaya plants with bacterially-expressed ihpRNA-CP279 targeting the PRSV CP gene can interfere with virus infection. This work lays a foundation for developing a non-transgenic approach to control PRSV by directly spraying plants with ihpRNA or crude bacterial extract preparations.

  13. Low-dose gamma irradiation following hot water immersion of papaya (Carica papaya linn.) fruits provides additional control of postharvest fungal infection to extend shelf life

    Science.gov (United States)

    Rashid, M. H. A.; Grout, B. W. W.; Continella, A.; Mahmud, T. M. M.

    2015-05-01

    Low-dose gamma irradiation (0.08 kGy over 10 min), a level significantly below that required to satisfy the majority of international quarantine regulations, has been employed to provide a significant reduction in visible fungal infection on papaya fruit surfaces. This is appropriate for local and national markets in producer countries where levels of commercial acceptability can be retained despite surface lesions due to fungal infection. Irradiation alone and in combination with hot-water immersion (50 °C for 10 min) has been applied to papaya (Carica papaya L.) fruits at both the mature green and 1/3 yellow stages of maturity. The incidence and severity of surface fungal infections, including anthracnose, were significantly reduced by the combined treatment compared to irradiation or hot water treatment alone, extending storage at 11 °C by 13 days and retaining commercial acceptability. The combined treatment had no significant, negative impact on ripening, with quality characteristics such as surface and internal colour change, firmness, soluble solids, acidity and vitamin C maintained at acceptable levels.

  14. Chemical and morphological characterization of Costa Rican papaya (Carica papaya L.) hybrids and lines with particular focus on their genuine carotenoid profiles.

    Science.gov (United States)

    Schweiggert, Ralf M; Steingass, Christof B; Esquivel, Patricia; Carle, Reinhold

    2012-03-14

    Papaya (Carica papaya L.) F1 hybrids and inbred lines grown in Costa Rica were screened for morphological and nutritionally relevant fruit traits. The qualitative composition of carotenoids showed great similarity, being mostly composed of free and esterified β-cryptoxanthins accompanied by β-carotene, lycopene, and biosynthetic precursors. High levels of (all-E)-lycopene and its isomers were distinctive for red-fleshed hybrids, whereas yellow-fleshed fruits were virtually devoid of lycopenes. Because carotenoid levels among the investigated hybrids and lines differed significantly, this study supports the hypothesis of an exploitable genetic variability, and a potential heterotic effect regarding carotenoid expression may be instrumental in papaya-breeding programs. Due to significantly higher levels of provitamin A carotenoids and coinciding high levels of total lycopene, particularly red-fleshed hybrids might represent prospective sources of these compounds. Furthermore, the nutritional value of some genotypes was boosted by substantial amounts of ascorbic acid (up to 73 mg/100 g of fresh weight), which correlated to total soluble solids (R(2) = 0.86).

  15. Estudio de la conservación de la papaya (Carica papaya L. asociado a la aplicación de películas comestibles

    Directory of Open Access Journals (Sweden)

    Alessandra Almeida Castro

    2011-06-01

    Full Text Available Brasil es un importante productor de papaya, pero sólo una pequeña parte se exporta. Esta fruta es altamente perecedera, por lo que el control de la maduración es esencial para aumentar la vida útil, tanto para el mercado interno como para la exportación. Una alternativa que ha cobrado impulso en el mercado es el uso de películas comestibles biodegradables, entre ellas la película producida a partir de almidón de yuca. En este estudio se evaluó el efecto de una película de almidón de yuca (2 % sobre la papaya almacenadas a temperatura ambiental (25 ± 2 ºC y a 8 °C, y 82 % de humedad relativa durante 6 días de almacenamiento. Se realizaron análisis de pérdida de peso, color, pH, acidez, carotenoides totales, vitamina C y sólidos solubles totales, cada tres días de almacenamiento. La película comestible de almidón de yuca resultó ser una buena alternativa para la preservación de la papaya durante seis días de almacenamiento, en relación a los parámetros pH, grados Brix, acidez y carotenoides.

  16. Estudio de la conservación de la papaya (Carica papaya L. asociado a la aplicación de películas comestibles

    Directory of Open Access Journals (Sweden)

    Alessandra Almeida Castro

    2011-01-01

    Full Text Available Brasil es un importante productor de papaya, pero sólo una pequeña parte se exporta. Esta fruta es altamente perecedera, por lo que el control de la maduración es esencial para aumentar la vida útil, tanto para el mercado interno como para la exportación. Una alternativa que ha cobrado impulso en el mercado es el uso de películas comestibles biodegradables, entre ellas la película producida a partir de almidón de yuca. En este estudio se evaluó el efecto de una película de almidón de yuca (2 % sobre la papaya almacenadas a temperatura ambiental (25 ± 2 ºC y a 8 °C, y 82 % de humedad relativa durante 6 días de almacenamiento. Se realizaron análisis de pérdida de peso, color, pH, acidez, carotenoides totales, vitamina C y sólidos solubles totales, cada tres días de almacenamiento. La película comestible de almidón de yuca resultó ser una buena alternativa para la preservación de la papaya durante seis días de almacenamiento, en relación a los parámetros pH, grados Brix, acidez y carotenoides.

  17. Potencial antifúngico de extractos de cuatro especies vegetales sobre el crecimiento de Colletotrichum gloeosporioides en papaya (Carica papaya en poscosecha

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    Nadia Landero Valenzuela

    2013-06-01

    Full Text Available El manejo de antracnosis por Colletotrichum gloeosporioides es el problema más importante en poscosecha de frutos tropicales. La actividad antifúngica de diferentes extractos vegetales fue evaluada in vitro e in vivo para controlar la antracnosis poscosecha en papaya. Extractos de ajo (Allium sativum (10 y 15 % y canela (Cinnamomum zeylanicum (0,0050; 0,0100; 0,0150 % mostraron efecto fungicida en contra de C. gloeosporioides para supresión de crecimiento micelial (100 %, inhibición de germinación (100 % y esporulación del hongo (100 %, considerándose estos extractos como los más promisorios para inhibir el desarrollo del hongo in vitro. Estudios in vivo también revelaron que los extracto de ajo (11,74 % y canela a dosis de 0,0054 % aplicada antes y al mismo tiempo de la inoculación con C. gloeosporioides, fueron las dosis óptimas para el control (severidad de la antracnosis en frutos de papaya artificialmente inoculados, mientras que a mayores dosis la severidad aumentó. La variable fitotoxicidad arrojó resultados consistentes con lo anterior, comprobándose que al aumentar las dosis de extracto de canela se presentó cambio de color en los frutos. Los resultados sugieren la posibilidad de usar estos extractos a dosis adecuadas como biofungicidas para controlar antracnosis en papaya en poscosecha.

  18. Chemical Characterization and in Vitro Cytotoxicity on Squamous Cell Carcinoma Cells of Carica Papaya Leaf Extracts

    Directory of Open Access Journals (Sweden)

    Thao T. Nguyen

    2015-12-01

    Full Text Available In traditional medicine, Carica papaya leaf has been used for a wide range of therapeutic applications including skin diseases and cancer. In this study, we investigated the in vitro cytotoxicity of aqueous and ethanolic extracts of Carica papaya leaves on the human oral squamous cell carcinoma SCC25 cell line in parallel with non-cancerous human keratinocyte HaCaT cells. Two out of four extracts showed a significantly selective effect towards the cancer cells and were found to contain high levels of phenolic and flavonoid compounds. The chromatographic and mass spectrometric profiles of the extracts obtained with Ultra High Performance Liquid Chromatography-Quadrupole Time of Flight-Mass Spectrometry were used to tentatively identify the bioactive compounds using comparative analysis. The principal compounds identified were flavonoids or flavonoid glycosides, particularly compounds from the kaempferol and quercetin families, of which several have previously been reported to possess anticancer activities. These results confirm that papaya leaf is a potential source of anticancer compounds and warrant further scientific investigation to validate the traditional use of papaya leaf to treat cancer.

  19. Biodiesel Production by Enzymatic Transesterification of Papaya Seed Oil and Rambutan Seed Oil

    Directory of Open Access Journals (Sweden)

    C. S. Wong

    2014-12-01

    Full Text Available Biodiesel production from vegetable oil has gained attention as an alternative fuel to minimize the usage of fossil fuels and reduce greenhouse gases pollution. In Malaysia, oils from local fruit seeds of papaya and rambutan are potential feedstock for biodiesel production due to their high lipid contents and easily available. In the present study, papaya and rambutan seed oils were extracted via soxhlet apparatus using n-hexane and the oil yields were in between 34–40%. The extracted oils were subjected to enzymatic transesterification by the immobilized Candida rugosa lipase as a catalyst under room temperature with varies molar ratios of methanol to oil. The highest biodiesel yield for papaya seed oil and rambutan seed oil was found to be 96% and 89% at methanol-to-oil ratios of 6:1 and 8:1, respectively. Results also showed a higher biodiesel yield using lipase immobilized on the magnetic particles as the heterogeneous catalyst compared to the yield obtained using free enzyme as the homogeneous catalyst. The properties of biodiesel such as density, acid value, iodine value and cetane number were analyzed and found to meet the European Standard of Biodiesel. The study shows that papaya and rambutan seed oils have the potential to be used as alternative feedstock for biodiesel production than the full dependence on palm oil in Malaysia.

  20. Assessment of the Anti-Protozoal Activity of Crude Carica papaya Seed Extract against Trypanosoma cruzi

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    Karla Y. Acosta-Viana

    2013-10-01

    Full Text Available In order to determine the in vivo activity against the protozoan Trypanosoma cruzi, two doses (50 and 75 mg/kg of a chloroform extract of Carica papaya seeds were evaluated compared with a control group of allopurinol. The activity of a mixture of the three main compounds (oleic, palmitic and stearic acids in a proportion of 45.9% of oleic acid, 24.1% of palmitic and 8.52% of stearic acid previously identified in the crude extract of C. papaya was evaluated at doses of 100, 200 and 300 mg/kg. Both doses of the extracts were orally administered for 28 days. A significant reduction (p < 0.05 in the number of blood trypomastigotes was observed in animals treated with the evaluated doses of the C. papaya extract in comparison with the positive control group (allopurinol 8.5 mg/kg. Parasitemia in animals treated with the fatty acids mixture was also significantly reduced (p < 0.05, compared to negative control animals. These results demonstrate that the fatty acids identified in the seed extracts of C. papaya (from ripe fruit are able to reduce the number of parasites from both parasite stages, blood trypomastigote and amastigote (intracellular stage.

  1. PHYSICO-CHEMICAL AND PHYTOCHEMICAL EVALUATION OF CARICA PAPAYA LINN. UNRIPE FRUITS

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    Anjum Varisha

    2013-08-01

    Full Text Available Present study was performed for the development of quality standards of Carica papaya Linn. commonly known as Papita belonging to family Caricaceae is well known for its exceptional nutritional and medicinal properties. The study comprises of physico-chemical and phytochemical evaluation to confirm purity and authenticity of Carica papaya L. unripe fruit using WHO guidelines. Microscopy of the fruit showed presence thick cuticle, parenchyma, epicarp, mesocarp endocarp, calcium oxalate crystals, laticifers, etc. Successive extractive value and hot extractive value was found highest in alcoholic extract 48.34 % and 44.90 % respectively (on dry weight basis. Mean ash values (% are 8.63 (total, 0.79 (acid insoluble ash, and 5.30 (water soluble ash and moisture content was found to be 9.41 % and the phytochemical analysis revealed the presence of carbohydrates, terpenoids, flavonoids, phenolic compounds in different extracts of Carica papaya L. fruit. TLC fingerprinting profile of different extracts were also developed and quantification of β-carotene was also done by using nhexane:acetone (8.5:1.5 as a mobile phase at 415 nm and found to be higher in pet ether Carica papaya L. fruit extract 1.55 % w/w.

  2. Molecular and Clinical Effects of Green Tea and Fermented Papaya Preparation on Diabetes and Cardiovascular Diseases

    Science.gov (United States)

    2014-11-14

    Assess the Effect of Green Tea on Diabetes; Assess the Effect of Fermented Papaya Pretration on Diabetes; Effects of Green Tea and FPP on C-reactive Proteins; Effects of Green Tea and FPP of Lipid Profiles in Diabetes; Effect of Green Tea and FPP on Atheroma Formation

  3. Crystallization and preliminary X-ray diffraction studies of the glutaminyl cyclase from Carica papaya latex

    Energy Technology Data Exchange (ETDEWEB)

    Azarkan, Mohamed [Laboratoire de Chimie Générale I, Faculté de Médecine-ULB CP609, 808 Route de Lennik, B-1070 Brussels (Belgium); Clantin, Bernard; Bompard, Coralie [CNRS-UMR 8525, Institut de Biologie de Lille, BP 477, 1 Rue du Professeur Calmette, F-59021 Lille (France); Belrhali, Hassan [EMBL Grenoble Outstation, 6 Rue Jules Horowitz, BP 181, F-38042 Grenoble CEDEX 9 (France); Baeyens-Volant, Danielle [Laboratoire de Chimie Générale I, Faculté de Médecine-ULB CP609, 808 Route de Lennik, B-1070 Brussels (Belgium); Looze, Yvan [Laboratoire de Chimie Générale, Institut de Pharmacie-ULB CP206/04, Boulevard du Triomphe, B-1050 Brussels (Belgium); Villeret, Vincent, E-mail: vincent.villeret@ibl.fr [CNRS-UMR 8525, Institut de Biologie de Lille, BP 477, 1 Rue du Professeur Calmette, F-59021 Lille (France); Wintjens, René, E-mail: vincent.villeret@ibl.fr [Laboratoire de Chimie Générale, Institut de Pharmacie-ULB CP206/04, Boulevard du Triomphe, B-1050 Brussels (Belgium); Laboratoire de Chimie Générale I, Faculté de Médecine-ULB CP609, 808 Route de Lennik, B-1070 Brussels (Belgium)

    2005-01-01

    The glutaminyl cyclase isolated from C. papaya latex has been crystallized using the hanging-drop method. Diffraction data have been collected at ESRF beamline BM14 and processed to 1.7 Å resolution. In living systems, the intramolecular cyclization of N-terminal glutamine residues is accomplished by glutaminyl cyclase enzymes (EC 2.3.2.5). While in mammals these enzymes are involved in the synthesis of hormonal and neurotransmitter peptides, the physiological role played by the corresponding plant enzymes still remains to be unravelled. Papaya glutaminyl cyclase (PQC), a 33 kDa enzyme found in the latex of the tropical tree Carica papaya, displays an exceptional resistance to chemical and thermal denaturation as well as to proteolysis. In order to elucidate its enzymatic mechanism and to gain insights into the structural determinants underlying its remarkable stability, PQC was isolated from papaya latex, purified and crystallized by the hanging-drop vapour-diffusion method. The crystals belong to the orthorhombic space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 62.82, b = 81.23, c = 108.17 Å and two molecules per asymmetric unit. Diffraction data have been collected at ESRF beamline BM14 and processed to a resolution of 1.7 Å.

  4. Chemical Characterization and in Vitro Cytotoxicity on Squamous Cell Carcinoma Cells of Carica papaya Leaf Extracts.

    Science.gov (United States)

    Nguyen, Thao T; Parat, Marie-Odile; Hodson, Mark P; Pan, Jenny; Shaw, Paul N; Hewavitharana, Amitha K

    2015-12-24

    In traditional medicine, Carica papaya leaf has been used for a wide range of therapeutic applications including skin diseases and cancer. In this study, we investigated the in vitro cytotoxicity of aqueous and ethanolic extracts of Carica papaya leaves on the human oral squamous cell carcinoma SCC25 cell line in parallel with non-cancerous human keratinocyte HaCaT cells. Two out of four extracts showed a significantly selective effect towards the cancer cells and were found to contain high levels of phenolic and flavonoid compounds. The chromatographic and mass spectrometric profiles of the extracts obtained with Ultra High Performance Liquid Chromatography-Quadrupole Time of Flight-Mass Spectrometry were used to tentatively identify the bioactive compounds using comparative analysis. The principal compounds identified were flavonoids or flavonoid glycosides, particularly compounds from the kaempferol and quercetin families, of which several have previously been reported to possess anticancer activities. These results confirm that papaya leaf is a potential source of anticancer compounds and warrant further scientific investigation to validate the traditional use of papaya leaf to treat cancer.

  5. Line 63-1: A New Virus-resistant Transgenic Papaya

    NARCIS (Netherlands)

    Tennant, P.; Souza, M.T.; Fitch, M.M.; Manshardt, R.; Slightom, J.L.; Gonsalves, D.

    2005-01-01

    The disease resistance of a transgenic line expressing the coat protein (CP) gene of the mild strain of the papaya ringspot virus (PRSV) from Hawaii was further analyzed against PRSV isolates from Hawaii and other geographical regions. Line 63-1 originated from the same transformation experiment tha

  6. Chemical treatment of papaya seeds aiming at long-term storage and control of damping off

    Directory of Open Access Journals (Sweden)

    Silvia de Carvalho Campos Botelho

    2014-06-01

    Full Text Available Damping off is a nursery disease of great economic importance in papaya and seed treatment may be an effective measure to control. The aim of this work was to evaluate the quality of papaya seeds treated with fungicides and stored under two environmental and packaging conditions. Additionally, the efficiency of fungicide treatments in the control of damping-off caused by Rhizoctonia solani was evaluated. Papaya seeds were treated with the fungicides Captan, Tolylfluanid and the mixture Tolylfluanid + Captan (all commercial wettable powder formulations. Seeds of the control group were not treated. The seeds were stored for nine months in two conditions: packed in aluminum coated paper and kept at 7 ± 1ºC and in permeable kraft paper and kept in non-controlled environment. At the beginning of the storage and every three months the seed quality (germination and vigor tests, emergence rate index, height, dry mass and damping of plants in pre and post-emergence (in contaminated substrate and mycelia-free substrate were analyzed. Both storage conditions as well as the fungicide treatments preserved the germination and seed vigor. In the infested substrate, seedling emergence was favored by fungicides, but in post-emergence, fungicides alone did not control the damping off caused by R. solani. Symptoms of damping off were not observed in the clean substrate. The results showed that the fungicide treatments may be used to pretreat papaya seed for long-term storage and commercialization.

  7. Purification of papain from fresh latex of Carica papaya

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    Rubens Monti

    2000-01-01

    Full Text Available In the present study we wish to report a method of crystallizing papain from fresh papaya latex which gave higher yields than previously reported. This method does not involve the use of sulphydryl reagents. The papain thus obtained is practically pure and shows a single band when submitted to electrophoresis on polyacrylamide gel, and is identical to the papain obtained by other methods. In routine enzymatic assays, specific activity was measured using Z-gly-pNP and BAEE as substrates. Papain crystallized by this method, without the use of high concentrations of salts or thiol-containing substances such as cysteine and dithiothreitol, is obtained in the form of a complex with natural inhibitors existent in latex which can be removed by dialysis.No presente trabalho apresenta-se um método de cristalização da papaína oriunda do látex fresco de mamão, o qual apresenta uma alta produtividade em relação aos métodos previamente descritos. A metodologia aqui descrita não envolve o uso de reagentes sulfidrílicos, a papaína foi obtida de forma praticamente pura, apresentando uma simples banda quando submetida a eletroforese, e com propriedades idênticas àquelas obtidas por outros métodos. A atividade específica foi determinada utilizando Z-gly-pNP e BAEE como substrato. A papaína obtida por essa metodologia, sem uso de substâncias tais como cisteína e ditiotreitol, apresenta-se na forma de um complexo com inibidores naturais, os quais podem ser removidos por diálise.

  8. Asociación entre envejecimiento exitoso y participación social en personas mayores chilenas

    Directory of Open Access Journals (Sweden)

    Lorena Gallardo-Peralta

    Full Text Available Objetivos: Analizar la asociación entre la participación social y el envejecimiento exitoso en una muestra representativa de personas mayores chilenas. Método: Se dispone de una muestra de 777 personas mayores chilenas. Se utilizó el Inventario de envejecimiento exitoso (SAI y todos los sujetos de la muestra firmaron una carta de consentimiento informado. Se realizaron análisis bivariados (prueba t de Student para muestras independientes y prueba de χ² a través de los programas SPSS (versión 23. Resultados: Se confirma la asociación entre participación social y envejecimiento exitoso; asimismo, los resultados muestran diferencias estadísticamente significativas entre las personas mayores que participan y los que no participan en el proceso de envejecer con éxito. Quienes participan en grupos sociales envejecen con éxito. Conclusiones: La participación social es un recurso psicosocial relevante en la intervención social gerontológica, dada su asociación positiva con el bienestar general de las personas mayores.

  9. A STUDY OF PAPAYA EXTRACT IN THE TREATMENT OF LOW PLATELET COUNT

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    Sakuntala Putrevu

    2016-09-01

    Full Text Available BACKGROUND Thrombocytopenia is defined as platelet count less than one and half lakh per mm cube of blood. Thrombocytopenia has been documented in patients with infectious mononucleosis, cytomegalovirus, and varicella zoster infections. Hepatitis C, tuberculosis and human immunodeficiency virus also have been reported in the causes list. Thrombocytopenia is a well-known complication of chronic lymphocytic leukaemia, although it may not be encountered as easily as seen in autoimmune haemolytic anaemias in these patients. It has also been reported in patients with other lymphoproliferative disorders including Hodgkin’s disease. Thrombocytopenia in patients with a variety of solid tumours has also been thought to most likely be immune mediated. Thrombocytopenia may accompany Graves’ disease and Hashimoto’s thyroiditis, but it is not certain that it is immunologically mediated or not. In our country, Dengue and malaria remain the predominant cause for Thrombocytopenia. Chymopapain and papain are the two important compound that are present in papaya. Lipase, a hydrolase, which is tightly bonded to the waterinsoluble fraction of crude papain is also seen in papaya. The papaya seeds and fruits have excellent antibiotic properties. The consumption of unripe and semi-ripe papaya fruits could be dangerous during pregnancy as it contains papain which may cause natural uterine contraction and may lead to abortions. It is reported that the plant extracts of papaya have maximum activity against dengue virus. It also has been reported that the methyl gallate of plant origin interacts with herpes simplex virus and causes its destruction. Majority of the papaya plant parts is known to have antimicrobial property. This study puts in a sincere effort to check the effects of papaya extract in the treatment of low platelet count. This study is intended to help the practising physicians to understand the benefits of the use of papaya extract when using in the

  10. The Complete Nucleotide Sequence and Biotype Variability of Papaya leaf distortion mosaic virus.

    Science.gov (United States)

    Maoka, Tetsuo; Hataya, Tatsuji

    2005-02-01

    ABSTRACT The complete nucleotide sequence of the genome of Papaya leaf distortion mosaic virus (PLDMV) was determined. The viral RNA genome of strain LDM (leaf distortion mosaic) comprised 10,153 nucleotides, excluding the poly(A) tail, and contained one long open reading frame encoding a polyprotein of 3,269 amino acids (molecular weight 373,347). The polyprotein contained nine putative proteolytic cleavage sites and some motifs conserved in other potyviral polyproteins with 44 to 50% identities, indicating that PLDMV is a distinct species in the genus Potyvirus. Like the W biotype of Papaya ringspot virus (PRSV), the non-papaya-infecting biotype of PLDMV (PLDMV-C) was found in plants of the family Cucurbitaceae. The coat protein (CP) sequence of PLDMV-C in naturally infected-Trichosanthes bracteata was compared with those of three strains of the P biotype (PLDMV-P), LDM and two additional strains M (mosaic) and YM (yellow mosaic), which are biologically different from each other. The CP sequences of three strains of PLDMV-P share high identities of 95 to 97%, while they share lower identities of 88 to 89% with that of PLDMV-C. Significant changes in hydrophobicity and a deletion of two amino acids at the N-terminal region of the CP of PLDMV-C were observed. The finding of two biotypes of PLDMV implies the possibility that the papaya-infecting biotype evolved from the cucurbitaceae-infecting potyvirus, as has been previously suggested for PRSV. In addition, a similar evolutionary event acquiring infectivity to papaya may arise frequently in viruses in the family Cucurbitaceae.

  11. Detection of Papaya ringspot virus type W infecting the cucurbit weed Cucumis melo var. dudaim in Florida

    Science.gov (United States)

    This is the first report of Papaya ringspot virus type W infecting Cucumis melo var. dudaim, a cucurbit weed, in Florida. It provides an overview of this virus reservoir for growers, extension workers, crop consultants and research and regulatory scientists....

  12. The use of sodium alginate-based coating and cellulose acetate in papaya post-harvest preservation

    Directory of Open Access Journals (Sweden)

    Denise Andrade Silva

    2014-02-01

    Full Text Available This study aimed to evaluate the ripening of papaya fruit (Carica papaya L. at room temperature (±25°C and10°C with 80% relative humidity, coated with edible film based on sodium alginate (1% and cellulose acetate film (3% by dipping the fruit in the suspensions for 1 min. On the application of the treatment and every three days during 12 days of storage, fruit were evaluated for weight loss, firmness, total carotenoid content, lycopene content and vitamin C content of the pulp. The cellulose acetate film extended the shelf-life of papayas, without affecting their quality. This treatment delayed fruit ripening, whose changes in all the parameters analyzed were significantly slower than fruit treated with sodium alginate-based coating. The coating with cellulose acetate at 3% was more effective in the preservation of papaya stored for 12 days under both temperatures.

  13. In vitro study of antiamoebic effect of methanol extract of mature seeds of Carica papaya on trophozoites of Entamoeba histolytica

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    Sujit Kumar Sarker

    2010-03-01

    Full Text Available Antiamoebic activity of methanol extract of mature seeds of Carica papaya was tested in vitro on axenic culture of Entamoeba histolytica using metronidazole as a reference amoebicidal agent. The MIC of seed extract was > 62.5 µg/mL as compared to < 0.8 µg/mL for metronidazole. The present study suggests that the mature seeds of C. papaya have antiamoebic effect but less pronounced than metronidazole.

  14. Interlaboratory validation data on real-time polymerase chain reaction detection for unauthorized genetically modified papaya line PRSV-YK

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    Kosuke Nakamura

    2016-06-01

    Real-time polymerase chain reaction (PCR detection method for unauthorized genetically modified (GM papaya (Carica papaya L. line PRSV-YK (PRSV-YK detection method was developed using whole genome sequence data (DDBJ Sequenced Read Archive under accession No. PRJDB3976. Interlaboratory validation datasets for PRSV-YK detection method were provided. Data indicating homogeneity of samples prepared for interlaboratory validation were included. Specificity and sensitivity test data for PRSV-YK detection method were also provided.

  15. Apatia e alheamento político numa sociedade paralisada: os limites da nova democracia chilena

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    Edward C. Epstein

    2001-01-01

    Full Text Available A noção de "sociedade paralisada" usada aqui para descrever a democracia chilena contemporânea pretende sugerir um determinado sistema político em que a probabilidade de mudança significativa resultante de eleições e negociações foi reduzida a um mínimo. Tendo em vista os dispositivos políticos restritivos impostos pelo regime militar de Pinochet à nova democracia, tais como a figura dos senadores nomeados e a lei eleitoral "binomial" que aumenta de modo muito desproporcional a representação do maior grupo minoritário pode-se afirmar que o poder político estava destinado a ser dividido quase igualmente entre as forças de centro e centro-esquerda agrupadas na coalizão da Concertación e seus oponentes da direita, num aparente desrespeito à vontade dos eleitores. A questão central deste artigo se relaciona com os efeitos prováveis que este imobilismo político pode ter sobre os chilenos à medida que eles se conscientizem de que as forças políticas que representam uma maioria popular têm capacidade limitada para realizar as mudanças desejadas. O tipo de efeito sugerido aqui se concentra na apatia e no alheamento político existentes.The notion of a "stalemate society" used here to describe contemporary Chilean democracy is meant to suggest a particular political system where the likelihood of significant change resulting through elections and bargaining has been reduced to a minimum. Given restrictive political provisions imposed by the Pinochet military regime upon the new democracy such as the constitutionally-sanctioned appointed Institutional Senators and the "binomial" election law which dramatically over-represents the largest minority group one can argue that political power was meant to be almost equally divided between those center and center-left forces grouped in the Concertación coalition and those opposing them on the political right, in seeming disregard of what the voters wanted. What is raised as the

  16. Caracterización del aceite de coquito de palma chilena (Jubaea chilensis

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    Torija, M. Esperanza

    2008-03-01

    Full Text Available The characteristics of the seeds from Chilean palm and the detailed composition of its fat are studied starting from 4 lots of seeds from the two regions being the major producers of Chilean palm seeds. From the proximate composition of the seeds a high content of fats is deduced with a mean value of 67.3%. The contents of proteins, carbohydrates and fibre were in the range of 7-11%. A detailed analysis of the fat indicates a high content of saturated fatty acids being capric, caprilic, lauric and miristic acids the major fatty acids. For this reason the presence of a high percentage of medium-chain triglycerides are detected in the triglyceride analysis. As for tocopherols, the fat contains low amounts of α-, γ- y δ-tocopherol with a total average of 84 mg/kg. Concerning phytosterols, the total content was around 1000 mg/kg being β-sitosterol and Δ7-estigmastenol the two major sterolsSe analizan las características generales del coquito de palma chilena y se estudia con detalle la composición de su aceite. Con este objetivo, se analizan 4 lotes de coquitos procedentes de las dos regiones de Chile donde existe mayor producción. La composición proximal del coquito indica un contenido muy mayoritario de grasa, con un promedio de 67,3% mientras los contenidos de proteínas, hidratos de carbono y fibra se encuentran entre el 7 y el 11%. Un análisis detallado de la grasa muestra un contenido elevado de ácidos grasos saturados (alrededor del 85% siendo los ácidos cáprico, caprílico, láurico y mirístico los que se encuentran en mayor concentración, lo que origina un elevado porcentaje de triglicéridos de cadena media. Respecto a los tocoferoles, el aceite contiene cantidades limitadas de α-, γ- y δ-tocoferol con un valor promedio total de 84 mg/kg, no detectándose la presencia de tocotrienoles. Respecto a los fitoesteroles, el contenido total es del orden de 1000 mg/kg siendo los esteroles mayoritarios el

  17. ‘Buscamos una voz que nos reciba’. Narrativa chilena reciente: lecturas cómplices

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    Daza D., Paulina

    2014-10-01

    Full Text Available This research explores certain suggested ways of reading recent Chilean narratives, focusing particularly on a corpus of works by four authors: Alvaro Bisama, Alejandra Costamagna, María José Viera-Gallo and Alejandro Zambra. It is proposed that from reception it is possible to specify certain ways of reading that manifest themselves in the degree of complicity achieved between the reader and the works, where this is defined by the reader’s cooperation or participation in the act of reading. The reader is drawn into the work by following the signs given in it, shifting from a critical to emotional reading by updating the stories through a close link between writer, the work and the reader. Thus the works are always read in a complicit way, but from different viewpoints and on different levels. Taking these nuances into account we aim to revise three ways of complicit reading: a through appropriation of the past, b politically and c dynamically.Esta investigación centra su estudio en algunas propuestas de lectura para la narrativa chilena reciente, especialmente un corpus compuesto por un conjunto de obras de cuatro autores: Álvaro Bisama, Alejandra Costamagna, María José Viera-Gallo y Alejandro Zambra. Se propone que desde la recepción es posible precisar algunas formas de lectura que se concretan pensando en el grado de complicidad que logra el lector con estas obras, determinada por su relación de colaboración o participación en el acto de leer. El lector se acercará más o menos a la obra siguiendo las señas propuestas en ella, así las formas de leer irán desde lo crítico hasta lo afectivo, actualizando los relatos a partir de un estrecho vínculo entre escritor, obra y lector. De esta forma la lectura siempre será cómplice, pero desde diferentes aspectos y en distintos grados. Teniendo en cuenta estos matices revisaremos tres formas de lectura cómplice: a de apropiación del pasado, b política y c dinámica.

  18. Diferencias entre la composición sectorial y ocupacional de las principales ciudades chilenas

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    Marcelo Lufín Varas

    2010-08-01

    Full Text Available La planifcación regional y urbana ha tendido a fjarse exclusivamente en la composición sectorial de las economías locales, asumiendo en forma implícita que la estructura de ocupaciones por actividad es similar a lo largo de un país. Si bien este supuesto parece ser cierto para el conjunto del tejido productivo, se encuentran diferencias signifcativas cuando se analizan ocupaciones específcas. Entre ellas destacan aquellas intensivas en conocimiento, las cuales tienden a estar sobrerrepresen-tadas en las principales ciudades. Se propone una metodología para el análisis de la relación existente entre la estructura de ocupaciones y la estructura sectorial y se aplica al estudio de las principales ciudades chilenas en 2002 a partir de la información del Censo de población, con el objetivo de estimar en qué medida estas estructuras divergen y si existen concentraciones relativas de ocupaciones, observándose que aquellas relacionadas con el conocimiento y la toma de decisiones empresariales tienen una presencia mayor que la esperada en el área metropolitana de Santiago.Regional and urban planning have tended to be exclusively focused on the industrial mix of local economies, implicitly assuming that the occupational mix of economic activities is similar throughout a country. Tis assumption seems to be right fom the perspective of the productive system as a whole, however signifcant diferences arise when analyzing certain occupations. Among them, knowledge-intensive occupations stand out and tend to be overrepresented in the main urban areas. A methodolog y is proposed to analyze the relationship between the occupational and industrial structures. Tis methodology is applied to the case of the principal Chilean cities in 2002 using data fom the population Census in order to estimate the extent to which these structures diverge and whether or not there are relative concentrations of occupations. Results show that the presence of managerial

  19. Rapid Construction of Stable Infectious Full-Length cDNA Clone of Papaya Leaf Distortion Mosaic Virus Using In-Fusion Cloning

    OpenAIRE

    Decai Tuo; Wentao Shen; Pu Yan; Xiaoying Li; Peng Zhou

    2015-01-01

    Papaya leaf distortion mosaic virus (PLDMV) is becoming a threat to papaya and transgenic papaya resistant to the related pathogen, papaya ringspot virus (PRSV). The generation of infectious viral clones is an essential step for reverse-genetics studies of viral gene function and cross-protection. In this study, a sequence- and ligation-independent cloning system, the In-Fusion® Cloning Kit (Clontech, Mountain View, CA, USA), was used to construct intron-less or intron-containing full-length ...

  20. Anatomia foliar de plantas transgênicas e não transgênicas de Carica papaya L. (Caricaceae Leaf anatomy of genetically modified and wild-type Carica papaya L. (Caricaceae

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    Marcos Vinicius Leal-Costa

    2010-06-01

    Full Text Available O mamoeiro, Carica papaya L. (Caricaceae é uma espécie americana, cujos frutos são largamente consumidos em todo mundo. Devido às perdas de produção provocadas por viroses e a dificuldade em controlá-las por métodos convencionais, a espécie tem sido alvo de pesquisas de melhoramento genético envolvendo transgenia para resistência a vírus. O presente trabalho descreve a anatomia foliar de plantas de mamoeiro convencional e transgênico resistente ao vírus da mancha anelar-Papaya ringspot virus (PRSV com inserção da capa protéica viral. As duas cultivares apresentam pecíolo com endoderme e fibras pericíclicas. As folhas são hipoestomáticas e dorsiventrais, com laticíferos acompanhando os feixes vasculares e grande concentração de idioblastos com drusas de oxalato de cálcio. A epiderme é glabra, possuindo estômatos anomocíticos e anisocíticos, com células de paredes anticlinais retas na face adaxial e levemente sinuosas na face abaxial. O presente trabalho concluiu que o processo de transformação genética não alterou as características anatômicas das folhas de C. papaya, servindo de subsídio para avaliação da conformidade anatômica da cultivar transgênica.Papaya, Carica papaya L. (Caricaceae, is an American species, consumed worldwide. A major limitation to papaya production is attack by viruses, like the papaya ringspot virus (PRSV. Papaya has been genetically modified to increase its resistance to PRSV. The aim of this research was to compare the leaf anatomy of wild-type and genetically modified (GM C. papaya plants to evaluate the influence of genetic modification on leaf anatomy. Wild-type and GM plants showed petiole with endodermis and pericycle fibers. The leaves are hypostomatic and dorsiventral, with laticifers along vascular system and abundant druses of calcium oxalate. The epidermis was glabrous and presented anomocytic and anisocytic stomata, straight anticlinal walls on the adaxial face and

  1. Cytogenetics of Chilean angiosperms: Advances and prospects Citogenética de angiospermas chilenas: Avances y proyecciones

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    PEDRO JARA-SEGUEL

    2012-03-01

    Full Text Available Cytogenetic data on Chilean angiosperms have been reported since at least eight decades ago; however, much of this information is disperse in diverse sources and is not readily available as a comprehensive document that allows having a general vision on advances and gaps in this matter. The goal of this paper is to summarize the advances and prospets on cytogenetic studies of the Chilean angiosperms based on compiled publications from 1929 to 2010. We found 78 publications supplied by four groups of Chilean researchers and some foreign specialists. Cytogenetic data have been reported for 139 Chilean angiosperm species (2.8 % of the total, which belong to 58 genera and 34 families. During 2001-2010 there was an increase in the number of publications, being available 40 reports including 95 additional species. Based on these data, we hope that such a trend can be maintained in the next decade if the current research groups and young specialists continue to be interested in the study of native plants.Los datos citogenéticos sobre angiospermas chilenas han sido reportados desde al menos ocho décadas atrás; sin embargo, mucha de esta información está dispersa en diversas fuentes y no está disponible como un documento completo que permita tener una visión general sobre los avances y vacíos en esta materia. El objetivo de este trabajo es resumir los avances y proyecciones sobre los estudios citogenéticos disponibles para angiospermas chilenas, basado en publicaciones recopiladas desde 1929 hasta el 2010. Nosotros encontramos 78 publicaciones aportadas por cuatro grupos de investigadores chilenos y por algunos especialistas extranjeros. Datos citogenéticos han sido reportados para 139 especies de angiospermas chilenas (2.8 % del total, las cuales pertenecen a 58 géneros y 34 familias. Durante los años 2001-2010, existió un incremento en el número de publicaciones estando disponibles 40 reportes que incluyen 95 especies adicionales. Basados

  2. Control de dos especies de Colletotrichum causantes de antracnosis en frutos de papaya Maradol Control of two species of Colletotrichum causing anthracnose in Maradol papaya fruits

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    Felipe Santamaría Basulto

    2011-10-01

    Full Text Available La antracnosis es una limitante de la vida útil de los frutos de papaya, es causada principalmente por Colletotrichum gloeosporioides, aunque es posible encontrar otras especies de Colletotrichum causando enfermedades en un mismo cultivo. Para el control de la antracnosis en Yucatán se recomiendan varios fungicidas, pero no todos están permitidos por la agencia de protección al ambiente (EPA de los Estados Unidos de América, principal país al cual se exporta la papaya yucateca. El presente estudio tuvo como objetivos identificar el agente causal de la antracnosis de papaya en Yucatán, evaluar el efecto de fungicidas que cuentan con registro de EPA sobre las especies que causan antracnosis y explorar el efecto de inductores a resistencia en el control de esta enfermedad. Durante marzo y mayo de 2006 se obtuvieron nueve aislamientos de Colletotrichum en frutos de tres regiones productoras de Yucatán, los cuales se identificaron mediante claves taxonómicas. La evaluación de los productos se hizo mediante bioensayos de sensibilidad in vitro y la efectividad en frutos inoculados, posteriormente en agosto de 2007, noviembre de 2007 y febrero de 2008, se evaluó el efecto de la aplicación poscosecha de los fungicidas e inductores de resistencia sobre la antracnosis en frutos infectados naturalmente desde campo. En las tres localidades se encontraron dos especies que causan antracnosis identificadas como C. gloeosporioides y C. dematium. En condiciones in vitro el desarrollo de C. gloeosporioides fue inhibido por prochloraz, ferbam, azoxystrobin, tryfloxystrobin y clorotalonil; el desarrollo de C. dematium fue inhibido sólo por prochloraz, ferbam y clorotalonil. En frutos inoculados prochloraz tuvo 100% de efectividad para las dos especies, mientras que azoxystrobin tuvo 87.5% de efectividad para C. gloeosporioides y 3.3% para C. dematium.Anthracnose is a limitation of papaya fruits' life, mainly caused by Colletotrichum gloeosporioides

  3. Fantasmas de rojo y azul. Los saqueos de las tropas chilenas en la guerra del Pacífico

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    Patricio Rivera Olguín

    2016-01-01

    Full Text Available La guerra del pacifico (1879-1884 se destaca como uno de los conflictos bélicos más feroces de América Latina, el cual dejó un triste y amargo recuerdo en quienes fueron vencidos (Perú y Bolivia y una aureola de triunfo y gloria en quienes vencieron (Chile. Sin embargo, existen numerosos hechos y pasajes omitidos por la historia tradicional del conflicto y que se refieren a los numerosos saqueos cometidos por las tropas chilenas, principalmente en ciudades y pueblos del Perú. Esta investigación presenta datos que verifican la existencia de estos hechos y se aproxima a la explicación de ellos, a través de la revisión de los registros dejados por los protagonistas del conflicto, en un intento de reconstruir la historia de la guerra, desde una perspectiva social.

  4. La familia Hemiaulaceae (Bacillariophyceae de las aguas marinas chilenas The family Hemiaulaceae (Bacillariophyceae from marine Chilean waters

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    PATRICIO RIVERA

    2003-12-01

    Full Text Available La familia Hemiaulaceae comprende cuatro géneros, señalados previamente para las aguas chilenas con las siguientes especies: Cerataulina pelagica (Cleve Hendey, Hemiaulus sinensis Greville, H. membranaceus Cleve, Climacodium biconcavum Cleve, Eucampia antarctica (Castracane Mangin, E. cornuta (Cleve Grunow y E. zodiacus Ehrenberg. Sin embargo, el análisis con microscopía fotónica y electrónica de muestras recolectadas en aguas marinas chilenas que contenían representantes de esta familia (incluyendo la mayoría de aquellas estudiadas anteriormente por otros investigadores nacionales reveló que (1 el género Cerataulina está representado por C. pelagica, distribuida a lo largo de la costa chilena entre Arica por el norte y el Estrecho de Magallanes por el sur, (2 el género Eucampia está representado por cuatro taxa: E. zodiacus f. cylindrocornis Syvertsen (señalada en trabajos anteriores como E. zodiacus f. zodiacus, E. zodiacus f. recta Rivera. Avaria & Cruces f. nov. (descrita aquí, E. cornuta y E. antarctica. Los primeros tres taxa se distribuyen en la zona central y norte de Chile, mientras que E. antarctica es propia de las aguas antárticas desde el Estrecho de Magallanes al sur, (3 las citas anteriores de Hemiaulus sinensis, H. membranaceus y Climacodium biconcavum para las aguas chilenas corresponden a determinaciones erróneas de Eucampia zodiacus f. recta, f. nov. Se entregan descripciones de los taxa encontrados y fotografías obtenidas con los microscopios fotónico y electrónicos que ilustran sus principales características morfológicasThe Family Hemiaulaceae comprises four genera, all of which have been previously reported for coastal waters off Chile, and represented by the following taxa: Cerataulina pelagica (Cleve Hendey, Hemiaulus sinensis Greville, H. membranaceus Cleve, Climacodium biconcavum Cleve, Eucampia antarctica (Castracane Mangin, E. cornuta (Cleve Grunow and E. zodiacus Ehrenberg. However, examination

  5. Análisis de la política de pago de dividendos en empresas chilenas

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    MAURICIO GUTIÉRREZ URZÚA

    2012-01-01

    Full Text Available Este artículo analiza la tasa de pago de dividendos de las empresas chilenas, considerando aspectos como el pago de dividendos obligatorios, la fuerte concentración de la propiedad, las oportunidades de crecimiento y la presencia de inversionistas institucionales. La investigación se realizó para el período 2001 a 2007, a través de regresiones lineales múltiples, con selección de variables mediante el criterio de stepwise y estimación de parámetros a través de máxima verosimilitud, en donde se encontró evidencia de la utilización de la tasa de pago de dividendos para entregar información sobre los proyectos futuros de la empresa y el tratamiento justo de los accionistas. También se destaca el control ejercido por los inversionistas institucionales.

  6. Interculturalidad y ciencias de la educación en lengua de señas chilena

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    Carolina-Alejandra-de-Lourdes Becerra-Sepúlveda

    2013-08-01

    Full Text Available Los paradigmas de intervención orales para la cultura sorda afectan la percepción sobre la lengua de señas Chilena . Esto provoca debates emanados de una subvaloración, dadas sus particularidades visuales: iconicidad e isomorfismo, subvaloración nacida de una incomprensión en la lectura de investigaciones internacionales (por escasez de estudios nacionales, especialmente acerca del lenguaje corporeizado. Esto se contradice con nuevas investigaciones en psicolingüística cognitiva acer-ca de embodied cognition . El abordaje de la LSCh a partir de nuevas teorías atraviesa la interculturalidad y educación a fin de proponerse la metáfora de “la cultura en el cuerpo” como modalidad de estudio contemporáneo.

  7. Confesiones subalternas: subjetivación, relato y culpa en tres novelas argentinas y chilenas de la posdictadura

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    Macarena Areco

    2014-12-01

    Full Text Available En este trabajo intento elaborar una reflexión acerca del régimen de repartición de culpas y de exención de responsabilidad en la posdictadura, a partir de la pregunta acerca de quiénes son los sujetos que se confiesan en algunas obras narrativas recientes. Para ello analizo tres novelas argentinas y chilenas - Ciencias morales (2007 de Martín Kohan, La vida doble (2010 de Arturo Fontaine y Carne de perra (2009 de Fátima Sime- protagonizadas por mujeres, que, de manera más o menos obligada o más o menos voluntaria, asumieron labores represivas e incluso criminales, en los setenta y ochenta en sus países.

  8. “Masonología chilena. O la porfiada memoria institucional de una élite decimonónica”

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    Felipe Santiago del Solar Guajardo

    2011-01-01

    Full Text Available El presente artículo busca analizar la estructura argumental de la historiografía masónica chilena. Para ello, se analizan los dos trabajos más importantes que se han escrito al respecto. Como hipótesis, planteamos que la historia de la francmasonería en Chile surge de diversas tradiciones, de donde destacan el proceso conflictual con la Iglesia católica, que la provee de un lenguaje para autodefinirse, y su doble naturaleza (latina y británica, que la obliga a través de la historia a legitimar su particularidad institucional y su participación pública.

  9. Characterization of the exogenous insert and development of event-specific PCR detection methods for genetically modified Huanong No. 1 papaya.

    Science.gov (United States)

    Guo, Jinchao; Yang, Litao; Liu, Xin; Guan, Xiaoyan; Jiang, Lingxi; Zhang, Dabing

    2009-08-26

    Genetically modified (GM) papaya (Carica papaya L.), Huanong No. 1, was approved for commercialization in Guangdong province, China in 2006, and the development of the Huanong No. 1 papaya detection method is necessary for implementing genetically modified organism (GMO) labeling regulations. In this study, we reported the characterization of the exogenous integration of GM Huanong No. 1 papaya by means of conventional polymerase chain reaction (PCR) and thermal asymmetric interlaced (TAIL)-PCR strategies. The results suggested that one intact copy of the initial construction was integrated in the papaya genome and which probably resulted in one deletion (38 bp in size) of the host genomic DNA. Also, one unintended insertion of a 92 bp truncated NptII fragment was observed at the 5' end of the exogenous insert. Furthermore, we revealed its 5' and 3' flanking sequences between the insert DNA and the papaya genomic DNA, and developed the event-specific qualitative and quantitative PCR assays for GM Huanong No. 1 papaya based on the 5' integration flanking sequence. The relative limit of detection (LOD) of the qualitative PCR assay was about 0.01% in 100 ng of total papaya genomic DNA, corresponding to about 25 copies of papaya haploid genome. In the quantitative PCR, the limits of detection and quantification (LOD and LOQ) were as low as 12.5 and 25 copies of papaya haploid genome, respectively. In practical sample quantification, the quantified biases between the test and true values of three samples ranged from 0.44% to 4.41%. Collectively, we proposed that all of these results are useful for the identification and quantification of Huanong No. 1 papaya and its derivates.

  10. Metáforas en Lengua de Señas Chilena Metaphors in Chilean Sign Language

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    Carolina Becerra

    2008-05-01

    Full Text Available Este estudio describe las características del lenguaje metafórico de personas sordas chilenas y su impacto en la comprensión lingüística. La relevancia de esta pregunta radica en la escasez de investigaciones realizadas, particularmente a nivel nacional. Se desarrolló un estudio cualitativo en base a análisis de videos de sujetos sordos en habla espontánea. Se confeccionó una lista de metáforas conceptuales y no conceptuales en Lengua de Señas Chilena. Posteriormente se evaluó su comprensión en un grupo de sujetos sordos, educados con modalidad comunicativa de lengua de señas. Los resultados obtenidos permiten observar la existencia de metáforas propias de la cultura sorda. Ellas serían coherentes con las particulares experiencias de los sujetos sordos y no necesariamente concuerdan con el lenguaje oral.The present study examined the characteristics of Chilean deaf people's metaphoric language and its relevance in linguistic comprehension. This key question is based in the scarcity of studies conducted in Chile. A qualitative study was developed, on the basis of analysis of videos of Chilean deaf people spontaneous sign language. A list of conceptual and no conceptual metaphors in Chilean sign language was developed. The comprehension of these metaphors was evaluated in a group of deaf subjets, educated using sign language communication. The results identify the existence of metaphors of the deaf culture. These methaphors would be coherent with the particular experiences of deaf subjets and do not necessarily agree with spoken language.

  11. DEL MALTRATO AL UXORICIDIO. LA VIOLENCIA «PUERTAS ADENTRO» EN LA ALDEA CHILENA TRADICIONAL (Siglo XIX

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    René Salinas Meza

    2011-10-01

    Full Text Available Este artículo pone de manifiesto el contexto en el cual las mujeres chilenas de las aldeas del siglo XIX son víctimas de diversos tipos de agresiones físicas, directas e indirectas, y psicológicas. Las conductas violentas de los agresores son explicadas en el marco de una comunidad que las reconoce como un componente habitual de la vida cotidiana. Así, se delimitan espacios específicos en los que se desenvuelven las relaciones de la gente, tales como el mundo urbano de la aldea y el mundo doméstico de la casa. En ambos, las personas construyen redes colectivas de solidaridad y fraternidad; pero, al mismo tiempo, desarrollan relaciones de competencia que son generadoras de roces y agresiones que ilustran la sociabilidad y el parentesco. Por otra parte, las mujeres agredidas confundieron a menudo la agresión con la aceptación de la autoridad masculina que permitía suponer la existencia de un matrimonio socialmente aceptado y cotidianamente bien llevado. La actitud de rebeldía femenina chocaba con el rechazo social y el ejercicio de un poder marital que se expresaba en conductas agresivas. Así, las relaciones en la pareja no fueron entre iguales, sino jerárquicas, imponiendo a las mujeres un rol subordinado y dependiente frente a los hombres, los que, a su vez, ostentaban la autoridad suficiente para controlarlas y castigarlas. Con la ayuda documental de un abundante número de expedientes judiciales se intenta comprender y explicar las conductas que asumen hombres y mujeres en la relación familiar desarrollada en las aldeas chilenas del siglo XIX.

  12. Características fenotípicas y agronómicas de seis genotipos de papaya (Carica papaya L.) de Tuxpan, Guerrero, México

    OpenAIRE

    José Ángel Alcántara Jiménez; Elías Hernández Castro; Sergio Ayvar Serna; Agustín Damián Nava; Tomás Brito Guadarrama

    2010-01-01

    En México la producción de papayo (Carica papaya L.) es afectada por factores genéticos, ambientales y bióticos. Destacan los daños provocados por el virus de la mancha anular, el cual reduce considerablemente la cantidad y calidad de la fruta; por eso es muy importante, conocer la productividad de los genotipos en un ambiente determinado. La presente investigación se realizó con el objetivo de evaluar el desarrollo y las características agronómicas de seis genotipos de papayo. El estudio se...

  13. Características fenotípicas y agronómicas de seis genotipos de papaya (Carica papaya L.) de Tuxpan, Guerrero, México

    OpenAIRE

    José Ángel Alcántara Jiménez; Elías Hernández Castro; Sergio Ayvar Serna; Agustín Damián Nava; Tomás Brito Guadarrama

    2010-01-01

    En México la producción de papayo (Carica papaya L.) es afectada por factores genéticos, ambientales y bióticos. Destacan los daños provocados por el virus de la mancha anular, el cual reduce considerablemente la cantidad y calidad de la fruta; por eso es muy importante, conocer la productividad de los genotipos en un ambiente determinado. La presente investigación se realizó con el objetivo de evaluar el desarrollo y las características agronómicas de seis genotipos de papayo. El estudio se ...

  14. Empleo de un Recubrimiento Formulado con Propóleos para el Manejo Poscosecha de Frutos de Papaya (Carica papaya L. cv. Hawaiana Use of a Coating Formulated with Propolis for Postharvest Handling of Papaya (Carica papaya L. cv. Hawaiian Fruits

    Directory of Open Access Journals (Sweden)

    Elizabeth Barrera Bello

    2012-06-01

    Full Text Available Resumen. El fruto de papaya es reconocido por las propiedades nutricionales y sensoriales; no obstante, su tiempo de vida útil en poscosecha es muy corto. En los últimos años, para incrementar la vida de almacenamiento del fruto se ha implementado el uso de recubrimientos como vehículos de agentes antimicrobianos y antioxidantes, entre otros. En este trabajo se comparó el efecto de dos recubrimientos; cera comercial (control y cera comercial conteniendo un extracto etanólico de propóleos (5% p/v, sobre la vida en poscosecha de frutos de papaya (Carica papaya L. cv. Hawaiana almacenados a temperatura ambiente (28 ± 2 ºC y humedad relativa entre 65 y 70%. El efecto de los recubrimientos se determinó mediante el índice de deterioro de los frutos y el recuento microbiano (mesófilos aerobios, mohos y levaduras. Adicionalmente, se evaluaron durante 12 días las propiedades fisicoquímicas de los frutos (cambio de color, textura, pH, acidez total titulable, pérdida de peso y sólidos solubles. Los resultados mostraron que las papayas tratadas con el recubrimiento formulado con el extracto de propóleos, presentó un menor deterioro en cuanto a su apariencia y mayor inhibición del crecimiento de microorganismos durante los primeros 6 días de evaluación en comparación con los frutos control; además, no se observaron diferencias, producto de los recubrimientos, en relación a las características fisicoquímicas de los frutos.Abstract. Papaya fruit is known for the nutritional and sensory properties; however, its postharvest shelf life is very short. In recent years, to extend the storage life of this fruit, the use of coatings as a carrier of antimicrobial agents and antioxidants, has been implemented. In this work, the effect of two coatings, commercial wax (control and commercial wax containing an ethanolic extract of propolis (5% w/v on the postharvest of papaya (Carica papaya L. cv. Hawaiian was compared. The fruits were stored at

  15. Beverages of lemon juice and exotic noni and papaya with potential for anticholinergic effects.

    Science.gov (United States)

    Gironés-Vilaplana, Amadeo; Valentão, Patrícia; Andrade, Paula B; Ferreres, Federico; Moreno, Diego A; García-Viguera, Cristina

    2015-03-01

    Lemon (Citrus limon (L.) Burm. f.) juice beverages enriched either with noni (Morinda citrifolia L.) (LN) or papaya (Carica papaya L.) (LP), were characterized by HPLC-DAD-ESI/MS(n), the antioxidant capacity was evaluated by (DPPH·), superoxide (O2(·-)), hydroxyl radicals (·OH) and hypochlorous acid (HOCl) assays, and their potential as acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitors was also assessed. The fruits are rich in a wide range of bioactive phenolics. Regarding DPPH·, ·OH and HOCl assays, the LP displayed strong activity, and LN was the most active against O2(·-). Concerning cholinesterases, LP was the most active, mainly due to lemon juice contribution. The effect on the cholinesterases was not as strong as in previous reports on purified extracts, but the bioactive-rich beverages offer the possibility of dietary coadjutants for daily consumption of health-promoting substances by adults with aging-related cognitive or physical disorders.

  16. AMELIORATION OF GENOTOXICITY BY PAPAYA EXTRACT INDUCED BY ARSENIC CONTAMINATED DRINKING WATER

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    DHARMSHILA KUMARI

    2013-01-01

    Full Text Available Arsenic is an established genotoxic carcinogen in human. Arsenic trioxide (0.04mg/animal when administeredorally daily to albino swiss mice for 15 continuous days, increased the incidence of abnormalities to 30.6% inmeiotic metaphase chromosome, 5.88% in the gross morphology of sperm head and decreased the sperm countper unit volume of caput epididymal suspension to 64.16 in comparison to the control. The concurrent treatmentof papaya fruit extract and arsenic trioxide significantly decreased the abnormalities to19.6% in meioticchromosome, 4.16% in the sperm head morphology and increased the mean count of sperm to 82.28. Theincrease in abnormality of meiotic chromosome as well as abnormality in gross morphology of the sperm headand decrease in sperm count, as observed upon arsenic trioxide treatment, were ameliorated by the aqueouspapaya fruit extract. Therefore, it is suggested that the papaya fruit extract may reduce the risk of arsenic-inducedgenotoxicity.

  17. Effect of the potassium permanganate during papaya fruit ripening: Ethylene production

    Science.gov (United States)

    Corrêa, S. F.; Filho, M. B.; da Silva, M. G.; Oliveira, J. G.; Aroucha, E. M. M.; Silva, R. F.; Pereira, M. G.; Vargas, H.

    2005-06-01

    The effect of potassium permanganate (KMnO4) on the ripening process of papaya fruits by monitoring the ethylene emission rates is reported. The ethylene emission was monitored by a photoacoustic spectrometer. Two experimental conditions were applied, being one of them just putting the fruit alone inside the sampling chamber and the second, modifying the atmosphere by the presence of KMnO4. The use of the ethylene absorber reduces the autocatalytic process of ethylene during papaya fruit ripening. For 20 g of KMnO4 the maximal intensity of the ethylene emission decreases by a factor two. Using the same amount of KMnO4, a reduction of about 2.2% in the concentration of ethylene for a mixture of 1ppmv of ethylene in synthetic air was observed.

  18. Uso de esterco associado à adubação mineral na produção de mudas de mamoeiro (Carica papaya L. The use of manure associated with mineral fertilization in papaya (Carica papaya L. seedling production

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    Regina Célia Faria Simão Canesin

    2006-12-01

    Full Text Available Na composição do substrato para produção de mudas de mamoeiro, recomenda-se o uso de adubação orgânica, que traz como vantagens a melhoria das características físicas, químicas e biológicas do solo, criando um ambiente favorável ao desenvolvimento inicial das mudas, bem como boa resposta do mamoeiro. Os materiais orgânicos desempenham ainda um importante papel na nutrição das plantas como fornecedores de nutrientes. Contudo, observa-se que existe uma grande variação nas recomendações e quase sempre a adubação orgânica está associada à adubação mineral. Neste contexto, o objetivo deste trabalho foi o de verificar os efeitos do uso de esterco de curral associado ou não à adubação mineral no substrato sobre a formação de mudas de mamoeiro. O Experimento foi conduzido na FEP/UNESP/Câmpus de Ilha Solteira-SP. O delineamento utilizado foi em blocos, em esquema de parcelas subdivididas, sendo parcelas as cultivares e subparcelas os substratos. Cada parcela com 5 repetições e 10 plantas úteis por subparcela. Para a comparação das médias, utilizou-se o teste de Tukey. Com base nos resultados obtidos, pode-se concluir que: a Na formação das mudas de mamoeiro, o esterco de curral pode ser utilizado sem a necessidade de adubação mineral com o superfosfato simples e o cloreto de potássio; b O esterco de curral foi capaz de fornecer às mudas de mamoeiro os nutrientes N, P, K, Ca, Mg e Cu, necessários para seu desenvolvimento até o transplantio para o campo.The use of organic fertilization is recommended to be added to the substrate for papaya seedling production, as it enhances the physical, chemical and biological characteristics of the soil, creating a favorable environment for the initial development of the plants, which results in better plant growth. The organic matter has also the important role of providing nutrients to the plants. However, there is great variation in the recommendations, and in most cases

  19. Development and validation of a multiplex reverse transcription PCR assay for simultaneous detection of three papaya viruses.

    Science.gov (United States)

    Tuo, Decai; Shen, Wentao; Yang, Yong; Yan, Pu; Li, Xiaoying; Zhou, Peng

    2014-10-21

    Papaya ringspot virus (PRSV), Papaya leaf distortion mosaic virus (PLDMV), and Papaya mosaic virus (PapMV) produce similar symptoms in papaya. Each threatens commercial production of papaya on Hainan Island, China. In this study, a multiplex reverse transcription PCR assay was developed to detect simultaneously these three viruses by screening combinations of mixed primer pairs and optimizing the multiplex RT-PCR reaction conditions. A mixture of three specific primer pairs was used to amplify three distinct fragments of 613 bp from the P3 gene of PRSV, 355 bp from the CP gene of PLDMV, and 205 bp from the CP gene of PapMV, demonstrating the assay's specificity. The sensitivity of the multiplex RT-PCR was evaluated by showing plasmids containing each of the viral target genes with 1.44 × 103, 1.79 × 103, and 1.91 × 102 copies for the three viruses could be detected successfully. The multiplex RT-PCR was applied successfully for detection of three viruses from 341 field samples collected from 18 counties of Hainan Island, China. Rates of single infections were 186/341 (54.5%), 93/341 (27.3%), and 3/341 (0.9%), for PRSV, PLDMV, and PapMV, respectively; 59/341 (17.3%) of the samples were co-infected with PRSV and PLDMV, which is the first time being reported in Hainan Island. This multiplex RT-PCR assay is a simple, rapid, sensitive, and cost-effective method for detecting multiple viruses in papaya and can be used for routine molecular diagnosis and epidemiological studies in papaya.

  20. Development and Validation of a Multiplex Reverse Transcription PCR Assay for Simultaneous Detection of Three Papaya Viruses

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    Decai Tuo

    2014-10-01

    Full Text Available Papaya ringspot virus (PRSV, Papaya leaf distortion mosaic virus (PLDMV, and Papaya mosaic virus (PapMV produce similar symptoms in papaya. Each threatens commercial production of papaya on Hainan Island, China. In this study, a multiplex reverse transcription PCR assay was developed to detect simultaneously these three viruses by screening combinations of mixed primer pairs and optimizing the multiplex RT-PCR reaction conditions. A mixture of three specific primer pairs was used to amplify three distinct fragments of 613 bp from the P3 gene of PRSV, 355 bp from the CP gene of PLDMV, and 205 bp from the CP gene of PapMV, demonstrating the assay’s specificity. The sensitivity of the multiplex RT-PCR was evaluated by showing plasmids containing each of the viral target genes with 1.44 × 103, 1.79 × 103, and 1.91 × 102 copies for the three viruses could be detected successfully. The multiplex RT-PCR was applied successfully for detection of three viruses from 341 field samples collected from 18 counties of Hainan Island, China. Rates of single infections were 186/341 (54.5%, 93/341 (27.3%, and 3/341 (0.9%, for PRSV, PLDMV, and PapMV, respectively; 59/341 (17.3% of the samples were co-infected with PRSV and PLDMV, which is the first time being reported in Hainan Island. This multiplex RT-PCR assay is a simple, rapid, sensitive, and cost-effective method for detecting multiple viruses in papaya and can be used for routine molecular diagnosis and epidemiological studies in papaya.

  1. PROCESO DE INFECCIÓN DE ANTRACNOSIS POR Colletotrichum truncatum EN PAPAYA MARADOL

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    INDIRA ROJO-BÁEZ

    Full Text Available RESUMEN Colletotrichum truncatum es un hongo patógeno causante de antracnosis en fruto de papaya (Carica papaya L. y responsable de pérdidas postcosecha. El objetivo de este trabajo fue inducir el proceso de infección de C. truncatum en frutos de papaya var. Maradol bajo condiciones controladas y decribir el progreso de la enfermedad mediante herramientas histopatológicas y microscopía óptica para elucidar el ciclo de vida del patógeno. La penetración directa de la cutícula por medio de apresorios ocurrió alrededor de las 30 h después de inoculación (hdi; las hifas intramurales crecieron en las paredes celulares de células epidérmicas a las 30-48 hdi; la colonización necrotrófica inició a las 72 hdi; hifas intracelulares crecieron dentro de celulas de parénquima causando una extensiva degradación celular lo cual se asocia a la producción de lesiones típicas de antracnosis. El rompimiendo de la cutícula del hospedero ocurrió entre las 96-148 hdi. El ciclo de vida del patógeno se completó a las 96 hdi con la formación de acérvulos. Las observaciones microscópicas en frutos de papaya revelaron que C. truncatum es un patógeno intramural subcuticular.

  2. Reasoned opinion on the modification of MRLs for spirodiclofen in strawberries bananas, avocado, mango and papaya

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    European Food Safety Authority

    2012-07-01

    Full Text Available

    In accordance with Article 6 of Regulation (EC No 396/2005, the Netherlands, herewith referred as the evaluating Member State (EMS, received an application from Bayer S.A.S-Bayer CropScience to modify the existing MRLs for spirodiclofen in strawberries and bananas and set import tolerances for papaya, avocado and mango. The Netherlands proposed to decrease the existing MRL for strawberries from 2 mg/kg to 0.02 mg/kg and to increase the MRL for banana from the limit of quantification 0.02* mg/kg to 0.3mg/kg. In order to accommodate the import of produce, the Netherlands proposed to set the MRL for papaya, mangos and avocados at 1.0 mg/kg. The Netherlands drafted an evaluation report according to Article 8 of Regulation (EC No 396/2005, which was submitted to the European Commission and forwarded to EFSA. According to EFSA the data are sufficient to derive MRL proposals of 0.3 mg/kg for the proposed use in banana and 1.5 mg/kg to accommodate the reported use in the USA on avocado, noting that the MRL in the country of origin is set at the level of 1 mg/kg. EFSA has some reservations regarding the proposal that the residue trial results for avocado might be extrapolated to propose MRLs on papaya and mango. The intended use on strawberries is not adequately supported by residue data. Based on the risk assessment results, EFSA concludes that the proposed use of spirodiclofen on strawberry, banana, avocado, mango and papaya will not result in consumer exposure exceeding the toxicological reference value and therefore is unlikely to pose a consumer health risk.

  3. Sensory acceptance of mixed nectar of papaya, passion fruit and acerola

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    Matsuura Fernando César Akira Urbano

    2004-01-01

    Full Text Available Nectars are beverages formulated with the juice or pulp of one or more fruits, plus water and sugar in concentrations resulting in a "ready-to-drink" product. Recently, the market for such products has greatly expanded. Fruit mixtures present a series of advantages, such as the combination of different aromas and flavors and the sum of their nutritional components. The objective of this work was to develop a nectar based on papaya pulp and passion fruit juice, enriched with the vitamin C present in acerola pulp, optimizing the formulation using sensory consumer tests and a response surface statistical methodology. Eleven formulations were prepared using different concentrations of papaya pulp and passion fruit juice and sucrose, and maintaining the concentration of acerola pulp constant. The sensory tests were carried out with 22 non-trained panelists using a structured 9-point hedonic scale to evaluate overall acceptance. The acceptance means were submitted to regression analysis, by first calculating a polynomial quadratic equation. A predictive model was adjusted considering only those parameters where P < 0.05, and a response surface was generated. The overall acceptance of nectars of different formulations varied from 5 ("neither liked nor disliked" to more than 7 ("liked moderately", showing that some products can be considered adequate to consumers, like the nectar produced with 37.5% papaya pulp, 7.5% passion fruit juice, and 5.0% acerola pulp, added of 15% sucrose. A quadratic predictive overall acceptance model, with a regression coefficient of 0.97 was obtained. The sensory acceptance of nectars was positively affected by increases in the concentrations of papaya pulp and of sucrose. Thus, some products presented good sensory acceptance suggesting commercial potential.

  4. Phenol induced by irradiation does not impair sensory quality of fenugreek and papaya

    Science.gov (United States)

    Chatterjee, Suchandra; Variyar, Prasad S.; Sharma, Arun

    2013-11-01

    The effect of radiation processing on the sensory quality of fenugreek and papaya exposed to doses in the range of 2.5-10 kGy and 100 Gy-2.5 kGy respectively was investigated. Despite an increase in the content of phenol in the volatile oil of these food products overall sensory quality of the irradiated and control samples was not significantly affected by radiation processing.

  5. Selecting black-spot resistant papaya genotypes derived from backcrossing and hybrids.

    Science.gov (United States)

    Poltronieri, T P S; Silveira, S F; Vivas, M; Santa Catarina, R; Cortes, D F M; Azevedo, A O N; Pereira, M G

    2017-02-23

    Papaya crop is important to Brazilian agribusiness. However, the expansion of papaya cultivation in the country is affected by the absence of commercial cultivars presenting good disease resistance. The black-spot caused by the fungus Asperisporium caricae is the most damaging foliar disease affecting Brazilian papaya crops. The use of genetically resistant cultivars is a promising strategy to reduce the dependence of papaya crops on fungicides. A field split-plot experiment was carried out in the municipality of Linhares, Espírito Santo State, and included 20 hybrids derived from the cross between 14 superior lines and four elite genotypes ('SS72/12', 'SEKATI', 'JS/12' and '41/7'), two commercial cultivars ('Golden' and 'Tainung 01'), and the superior line '36/7', which were evaluated for resistance to black-spot in the fruits and leaves. The treatments were arranged in a randomized block design with six repetitions of three plants per plot. The incidence and severity of black spot in the fruits and leaves were evaluated at three different times in the 2015-2016 crop season. Lines 4, 9, 21, and the parent SEKATI were notable for their capacity to reduce disease severity in the leaves and fruits. Lines 1, 2, 9, 16, and 19, and the parents 'SEKATI' and 'SS-72/12' had reduced disease incidence in their fruits. The most resistant hybrids 'SS-72/12 X 4', 'SS-72/12 X 6', 'SEKATI X 1', 'SEKATI X 2', 'SEKATI X 6', 'SEKATI X 9', and 'SEKATI X 20' presented negative heterosis values for improved black-spot resistance. The current study allowed the selection of black-spot resistant genotypes and hybrids, which presented a significantly reduced disease index in the field.

  6. GROWTH OF MACROBRACHIUM ROSENBERGII FED WITH MANGO SEED KERNEL, BANANA PEEL AND PAPAYA PEEL INCORPORATED FEEDS

    OpenAIRE

    P. Aarumugam; P. Saravana Bhavan; T. Muralisankar; N. Manickam; V. Srinevasan; S. Radhakrishnan

    2013-01-01

    The growth promoting potential of fruits wastes, mango seed kernel, banana peel and papaya peel on the freshwater prawn, Macrobrachium rosenbergii post larvae (PL) was evaluated. Basal diet equated to 35% protein was prepared by using soybean meal, groundnut oilcake, horse gram and wheat flour. Each fruit waste powder was separately incorporated with basal diet at a proportion of 10%. Sunflower oil was used as lipid source. Egg albumin and tapioca flour were used as binding agents. Vitamin B-...

  7. Bioseparation of papain from Carica papaya latex by precipitation of papain-poly (vinyl sulfonate) complexes.

    Science.gov (United States)

    Braia, Mauricio; Ferrero, Maximiliano; Rocha, María Victoria; Loureiro, Dana; Tubio, Gisela; Romanini, Diana

    2013-09-01

    The formation of insoluble complexes between enzymes and polyelectrolytes is a suitable technique for isolating these biomolecules from natural sources, because it is a simple and rapid technique that allows the concentration of the protein. This technique can be used in most purification protocols at the beginning of the downstream process. The aim of this investigation is to isolate papain from Carica papaya latex by precipitation of insoluble complexes between this enzyme and poly (vinyl sulfonate). The papain-poly (vinyl sulfonate) complex was insoluble at pH lower than 6, with a PVS/PAP stoichiometric ratio of 1:279. Ionic strength affected the complex formation. The presence of the polymer increased the enzymatic activity and protected the enzyme from autodegradation. The optimal conditions for the formation of insoluble papain-polyelectrolyte complex formation were applied to C. papaya latex and a high recovery was obtained (around 86%) and a purification factor around 2. This method can be applied as an isolation method of papain from C. papaya latex or as a first step in a larger purification strategy.

  8. Human sperm immobilization effect of Carica papaya seed extracts: an in vitro study

    Institute of Scientific and Technical Information of China (English)

    NirmalKLohiya; LalitKKothari; BManivannan; PradyumnaKMishra; NeelamPathak

    2000-01-01

    Aim: To examine if the seed extracts of Carica papaya, which showed antispermatogenic/sperm immobilization properties in animal models, could cause human sperm immobilization in vitro. Methods: Chloroform extract, benzene chromatographic fraction of the chloroform extract, its methanol and ethyl acetate sub-fractions and the isolated compounds from the sub-fractions i.e., ECP 1 & 2 and MCP 1 & 2, of the seeds of Cadca papaya were used at concentrations of 0.1%, 0.5%, 1% and 2%. Sperm motility was assessed immediately after addition of extracts and every 5 minutes thereafter for 30 minutes. Results: There were dose-dependent spermicidal effects showing an instant fall in the sperm motility to less than 20 % at 2 % concentration. Isolated compounds ECP 1 & 2 were more effective inducing a motility of less than 10%. Many of the spermatozoa became vibratory on the spot. Total inhibition of motility was observed within 20 - 25 min at all concentrations of all products. Scanning and transmission electron microscopy revealed deleterious changes in the plasma membrane of the head and mid-piece of spermatozoa. Sperm viability test and the number of abnormal spermatozoa after completion of incubation suggested that the spermatozoa were infertile. The effects were spermicidal but not spermiostatic as revealed by the sperm revival test. Conclusion: The results reveal spermicidal activity in vitro of the seed extracts of Carica papaya.

  9. Sequencing papaya X and Yh chromosomes reveals molecular basis of incipient sex chromosome evolution.

    Science.gov (United States)

    Wang, Jianping; Na, Jong-Kuk; Yu, Qingyi; Gschwend, Andrea R; Han, Jennifer; Zeng, Fanchang; Aryal, Rishi; VanBuren, Robert; Murray, Jan E; Zhang, Wenli; Navajas-Pérez, Rafael; Feltus, F Alex; Lemke, Cornelia; Tong, Eric J; Chen, Cuixia; Wai, Ching Man; Singh, Ratnesh; Wang, Ming-Li; Min, Xiang Jia; Alam, Maqsudul; Charlesworth, Deborah; Moore, Paul H; Jiang, Jiming; Paterson, Andrew H; Ming, Ray

    2012-08-21

    Sex determination in papaya is controlled by a recently evolved XY chromosome pair, with two slightly different Y chromosomes controlling the development of males (Y) and hermaphrodites (Y(h)). To study the events of early sex chromosome evolution, we sequenced the hermaphrodite-specific region of the Y(h) chromosome (HSY) and its X counterpart, yielding an 8.1-megabase (Mb) HSY pseudomolecule, and a 3.5-Mb sequence for the corresponding X region. The HSY is larger than the X region, mostly due to retrotransposon insertions. The papaya HSY differs from the X region by two large-scale inversions, the first of which likely caused the recombination suppression between the X and Y(h) chromosomes, followed by numerous additional chromosomal rearrangements. Altogether, including the X and/or HSY regions, 124 transcription units were annotated, including 50 functional pairs present in both the X and HSY. Ten HSY genes had functional homologs elsewhere in the papaya autosomal regions, suggesting movement of genes onto the HSY, whereas the X region had none. Sequence divergence between 70 transcripts shared by the X and HSY revealed two evolutionary strata in the X chromosome, corresponding to the two inversions on the HSY, the older of which evolved about 7.0 million years ago. Gene content differences between the HSY and X are greatest in the older stratum, whereas the gene content and order of the collinear regions are identical. Our findings support theoretical models of early sex chromosome evolution.

  10. Reduction of hydrogen peroxide-induced erythrocyte damage by Carica papaya leaf extract

    Institute of Scientific and Technical Information of China (English)

    Tebekeme Okoko; Diepreye Ere

    2012-01-01

    Objective: To investigate the in vitro antioxidant potential of Carica papaya (C. papaya) leaf extract and its effect on hydrogen peroxide-induced erythrocyte damage assessed by haemolysis and lipid peroxidation. Methods: Hydroxyl radical scavenging activities, hydrogen ion scavenging activity, metal chelating activity, and the ferrous ion reducing ability were assessed as antioxidant indices. In the other experiment, human erythrocytes were treated with hydrogen peroxide to induce erythrocyte damage. The extract (at various concentrations) was subsequently incubated with the erythrocytes and later analysed for haemolysis and lipid peroxidation as indices for erythrocyte damage. Results:Preliminary investigation of the extract showed that the leaf possessed significant antioxidant and free radical scavenging abilities using in vitro models in a concentration dependent manner (P<0.05). The extract also reduced hydrogen peroxide induced erythrocyte haemolysis and lipid peroxidation significantly when compared with ascorbic acid (P<0.05). The IC50 values were 7.33 mg/mL and 1.58 mg/mL for inhibition of haemolysis and lipid peroxidation, respectively. In all cases, ascorbic acid (the reference antioxidant) possessed higher activity than the extract. Conclusions:The findings show that C. papaya leaves possess significant bioactive potential which is attributed to the phytochemicals which act in synergy. Thus, the leaves can be exploited for pharmaceutical and nutritional purposes.

  11. Spatial and temporal diversity of begomoviral complexes in papayas with leaf curl disease.

    Science.gov (United States)

    Singh-Pant, P; Pant, P; Mukherjee, S K; Mazumdar-Leighton, S

    2012-07-01

    Old World, monopartite begomoviruses associated with satellite DNA β were observed in papaya showing symptoms of leaf curl disease sampled randomly over five years from within a radius of 250 km in north-central India. Three groups of DNA A sequences were evident. One group resembled chili leaf curl virus infecting tomatoes (ChiLCuV). Another group resembled tomato leaf curl New Delhi virus (ToLCuNDV). The third group was novel (tentatively named papaya leaf crumple virus, PaLCrV), with less than 89% identity to known begomovirus sequences in the GenBank database. At least seven DNA A sequences were putative recombinants. The AC4-encoding regions exhibited highest numbers of non-synonymous substitutions. Most DNA β sequences resembled tomato leaf curl virus-associated DNA βs. A few DNA β sequences were similar to that of croton yellow vein mosaic virus-associated DNA β (CroYVMVβ). One DNA β sequence was novel and showed papayas grown in plantations, kitchen gardens and feral patches in the region are vulnerable to disease outbreak. No geographic or temporal patterns were discernable in the distribution of these viruses.

  12. Antiinflammatory activity of the methanolic extract of the seeds ofCarica papaya in experimental animals

    Institute of Scientific and Technical Information of China (English)

    Amazu LU; Azikiwe CCA; Njoku CJ; Osuala FN; Nwosu PJC; Ajugwo AO; Enye JC

    2010-01-01

    Objective:To scientifically verify the claims of our traditional healers on the anti-inflammatory activity ofCarica papaya (C. papaya) and possibly deduce its activities.Methods:0.1 mL of fresh egg albumin was injected into the right hind-paw of adult white Wistar rats to induce inflammation an hour post intraperitoneal (IP) administration of50-200 mg/kg doses of the extract to3groups of5 rats per group. The 4th group of5 rats was used as negative control and received2 mL/kg(IP) of physiological saline, while the 5th group of5rats was used as positive-comparative control and received (IP) 150 mg/kg of aspirin. Increases in diameter of the paw were measured with the aid of Veneer Calipers before extract administration and at interval of30minutes post administration for further 2 hours. Percentage inhibition of oedema was calculated for each dose group and results were subjected to statistical analysis using studentt-test and analysis of variance(ANOVA).Results: All doses of extract showed a dose and time dependent inhibition effects of oedema(P<0.05).Conclusions:This work is at present though limited to animals, the anti-inflammatory activity of the seeds ofC. papaya is perhaps proven.

  13. Quality Characteristics, Nutraceutical Profile, and Storage Stability of Aloe Gel-Papaya Functional Beverage Blend

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    Pushkala Ramachandran

    2014-01-01

    Full Text Available Aloe vera gel, well known for its nutraceutical potential, is being explored as a functional ingredient in a wide array of health foods and drinks. Processing of exotic fruits and herbal botanicals into functional beverage is an emerging sector in food industry. The present study was undertaken to develop a spiced functional RTS beverage blend using Aloe gel (AG and papaya. Aloe gel (30%, papaya pulp (15%, spice extract (5%, and citric acid (0.1% were mixed in given proportion to prepare the blend with TSS of 15 °Brix. The product was bottled, pasteurized, and stored at room temperature. The quality characteristics and storage stability of the spiced beverage blend (SAGPB were compared with spiced papaya RTS beverage (SPB. Periodic analysis was carried out up to five months for various physicochemical parameters, sugar profile, bioactive compounds, microbial quality, instrumental color, and sensory acceptability. The SAGPB exhibited superior quality characteristics compared to SPB both in fresh and in stored samples. The SPB was acceptable up to four months and SAGPB for five months. The results indicate that nutraceutical rich AG could be successfully utilized to develop functional fruit beverages with improved quality and shelf life.

  14. LEAF GAS EXCHANGE CHARACTERISTICS OF FOUR PAPAYA GENOTYPES DURING DIFFERENT STAGES OF DEVELOPMENT

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    CAMPOSTRINI ELIEMAR

    2001-01-01

    Full Text Available In this research, was used four papaya (Carica papaya L. genotypes: three from the 'Solo ( Sunrise Solo TJ, Sunrise Solo 72/12 and Baixinho de Santa Amália group and one from the 'Formosa' group (Know-You 01. They were grown in plastic pots containing a sandy-clay-loam soil subjected to pH correction and fertilization, under greenhouse conditions. Throughout the experimental period plants were subjected to periodic irrigation to maintain the soil humitidy around field capacity. The experiment was conducted 73 days after sowing. In all genotypes, leaf gas exchange characteristics were determined. The net photosynthetic rate (A, mumol m-2 s-1 , stomatal conductance (g s mol m-2 s-1, leaf temperature (T I, 0C and intercellular carbon dioxide concentration (ci, muL L-1 on the 4th, 5th, 6th, 7th, 8th and 9th leaves from the plant apex were determined. No significant differences were observed for A, g s, c i, or Tl either among the leaves sampled from any of the genotypes. A was positively correlated with g s and in the other hand T I and g s were negatively correlated. The results suggest that, for 73 DAP, all the sampled papaya leaves functioned as sources of organs.

  15. Gastroprotective and Anti-ulcer activity of Aloe vera juice, Papaya fruit juice and Aloe vera and Papaya fruit combined juice in Ethanol induced Ulcerated Rats

    Directory of Open Access Journals (Sweden)

    S. Gopinathan

    2013-12-01

    Full Text Available Peptic ulcer is the most prevalent gastrointestinal disease. Even though a wide range of drugs are available for the treatment of peptic ulcer, but many of these do not fulfill all the requirements and have side effects. These factors have attracted researchers to investigate the natural products which have more efficacy, less side effects and less expensive for the treatment of peptic ulcer disease. In the present study the anti ulcer activity of (1 Aloe vera juice, (2 papaya fruit juice (3 Aloe vera and papaya fruit combined juice were investigated in the ethanol induced ulcerated rats. The administration of plant juices decreased the offensive factors like ulcer index and acid secretion and also reduced the amount of protein and carbohydrates in the stomach fluid. Further, plant juices increased the defensive factors like activity of oxidative enzymes such as superoxide dismutase and reduced glutathione. Activities of alkaline phosphatase and lipid peroxide were higher in the diseased condition and same were reduced after the treatment with plant juices. Content of haemoglobin and RBC and WBC counts were brought back to normalcy after the treatment with plant juices. The efficacy of plant juices was comparable with the reference drug- Ranitidine. The results of the present study reveal that the plant juices are having efficiency in the gastroprotective activity. It is recommended that the above said plant juices can be further studied for their anti ulcer efficacy in human subjects.

  16. Consideraciones sobre la utilización de diferentes densidades en el cultivo de papaya (Carica papaya, L. "Baixinho de Santa Amalia" en islas canarias

    Directory of Open Access Journals (Sweden)

    Pastor Mª Cristina Rodriguez

    2002-01-01

    Full Text Available El cultivo de papaya en Islas Canarias se ha extendido en los últimos años bajo condiciones de invernadero. La utilización de cultivares de papaya tipo "Baixinho de Santa Amalia" (mutante natural del cultivar "Sunrise" de porte bajo, emisión de la flor a corta altura y precocidad en la floración, resultan de gran interés sobre todo en esta clase de medios. Estas características fenológicas hacen posible el manejo del cultivo a mayores densidades que las empleadas con otros cultivares. Por lo tanto, se ha planteado este trabajo cuyo objetivo principal es determinar cual es el marco de plantación óptimo, que permita obtener mayores rendimientos sin depreciar la calidad del fruto. Para ello, se ha evaluado la producción tanto de las plantas hermafroditas como de las plantas femeninas durante dos ciclos de cultivo, así como las características organolépticas, grado de carpeloidía y deformación de los frutos. Los resultados indican que la densidad mayor, proporciona mejor comportamiento de las plantas así como mayor producción de frutos y menor porcentaje de fruta desechable.

  17. Ultrasound-assisted extraction (UAE) and solvent extraction of papaya seed oil: yield, fatty acid composition and triacylglycerol profile.

    Science.gov (United States)

    Samaram, Shadi; Mirhosseini, Hamed; Tan, Chin Ping; Ghazali, Hasanah Mohd

    2013-10-10

    The main objective of the current work was to evaluate the suitability of ultrasound-assisted extraction (UAE) for the recovery of oil from papaya seed as compared to conventional extraction techniques (i.e., Soxhlet extraction (SXE) and solvent extraction (SE)). In the present study, the recovery yield, fatty acid composition and triacylglycerol profile of papaya seed oil obtained from different extraction methods and conditions were compared. Results indicated that both solvent extraction (SE, 12 h/25 °C) and ultrasound-assisted extraction (UAE) methods recovered relatively high yields (79.1% and 76.1% of total oil content, respectively). Analysis of fatty acid composition revealed that the predominant fatty acids in papaya seed oil were oleic (18:1, 70.5%-74.7%), palmitic (16:0, 14.9%-17.9%), stearic (18:0, 4.50%-5.25%), and linoleic acid (18:2, 3.63%-4.6%). Moreover, the most abundant triacylglycerols of papaya seed oil were triolein (OOO), palmitoyl diolein (POO) and stearoyl oleoyl linolein (SOL). In this study, ultrasound-assisted extraction (UAE) significantly (p composition of papaya seed oil extracted by different extraction methods (SXE, SE and UAE) and conditions.

  18. Repeated dose 28-days oral toxicity study of Carica papaya L. leaf extract in Sprague Dawley rats.

    Science.gov (United States)

    Afzan, Adlin; Abdullah, Noor Rain; Halim, Siti Zaleha; Rashid, Badrul Amini; Semail, Raja Hazlini Raja; Abdullah, Noordini; Jantan, Ibrahim; Muhammad, Hussin; Ismail, Zakiah

    2012-04-10

    Carica papaya L. leaves have been used in ethnomedicine for the treatment of fevers and cancers. Despite its benefits, very few studies on their potential toxicity have been described. The aim of the present study was to characterize the chemical composition of the leaf extract from 'Sekaki' C. papaya cultivar by UPLC-TripleTOF-ESI-MS and to investigate the sub-acute oral toxicity in Sprague Dawley rats at doses of 0.01, 0.14 and 2 g/kg by examining the general behavior, clinical signs, hematological parameters, serum biochemistry and histopathology changes. A total of twelve compounds consisting of one piperidine alkaloid, two organic acids, six malic acid derivatives, and four flavonol glycosides were characterized or tentatively identified in the C. papaya leaf extract. In the sub-acute study, the C. papaya extract did not cause mortality nor were treatment-related changes in body weight, food intake, water level, and hematological parameters observed between treatment and control groups. Some biochemical parameters such as the total protein, HDL-cholesterol, AST, ALT and ALP were elevated in a non-dose dependent manner. Histopathological examination of all organs including liver did not reveal morphological alteration. Other parameters showed non-significant differences between treatment and control groups. The present results suggest that C. papaya leaf extract at a dose up to fourteen times the levels employed in practical use in traditional medicine in Malaysia could be considered safe as a medicinal agent.

  19. Effect of cassava starch-based edible coating incorporated with lemongrass essential oil on the quality of papaya MJ9

    Science.gov (United States)

    Praseptiangga, D.; Utami, R.; Khasanah, L. U.; Evirananda, I. P.; Kawiji

    2017-02-01

    Edible films and coatings have emerged as an alternative packaging in food applications and have received much attention due to their advantages. The incorporation of essential oils in film matrices to give antimicrobial properties had been observed recently, and could be used as promising preservation technology. In this study, cassava starch-based edible coating incorporated with lemongrass essential oil (1%) was applied by spraying and dipping methods to preserve papaya MJ9 during storage at room temperature. The quality of papaya MJ9 was analyzed based on its physicochemical and microbiological properties. The addition of lemongrass essential oil (1%) significantly inhibited the microbial growth on papaya MJ9 by reducing the value of total yeast and mold as compared to the control. This study also showed that for parameters of weight loss, total soluble solid, vitamin C, and total titratable acid, papaya MJ9 with cassava starch-based edible coating incorporated with lemongrass essential oil (1%) had the lower values than control, however, they had the higher value than control on firmness parameter. These results indicate that cassava starch-based edible coating incorporated with lemongrass essential oil (1%) can be used as an alternative preservation for papaya MJ9.

  20. Study about papaya (Carica papaya L.) conservation submitted to a combined treatment of irradiation and an edible film of quitosan; Estudo do tratamento combinado de radiacao ionizante e cobertura de quitosana em mamao papaia (Carica papaya L.)

    Energy Technology Data Exchange (ETDEWEB)

    Camargo, Rita Junqueira de

    2004-07-01

    In this research, on a first stage the effect of two treatments were studied: thermal, and a chitosan edible coating, combined with four different irradiation doses: 0,00 kGy, 0,50 kGy, 0,75 kGy and 1,00 kGy for effects in the ripening of papaya (Carica Papaya) cv. Golden. To evaluate the influence of these two factors in the physical-chemical characteristics of the fruit during ripening, analysis on color, both skin and pulp, firmness, soluble solids, pH and titrable acidity were performed. The incidence of fungi in fruit skin was also analyzed. The fruits were first maintained at 10 deg C chamber for fifteen days to simulate maritime transportation to the importer country and then transferred to a 25 deg C chamber aiming simulation of the commercialization conditions. From this first step, the conclusion was that chitosan in the concentration of 2% was not able to contain the fungi development and that it also influenced in a significant form the variable pH. A more expressive result was obtained for irradiation doses of 0,75 kGy and 1,00 kGy, as it was observed in the beginning the acceleration in ripening through the variables skin color and penetration energy of the fruit pulp. When storage temperature was changed to 25 deg C this situation inverted for these same variables and a two day delay was observed on the development of yellow color on the fruit skin and loss of pulp firmness. The variables titrable acidity, soluble solids and pulp color were not influenced by treatment or by irradiation. On a second stage, the fruits, in the same condition of the previous stage, were analyzed in relation of their mass and ripening stage using a ripening scale provided by the producer of the fruits for this experiment, as to evaluate the gradual development of fruit ripening according to the various treatments (chitosan 3%, thermal or control) and irradiation dose (0,00 kGy and 0,75 kGy). Confirming the results obtained in the first stage, the irradiation was able to

  1. Detection of platypus-type L/D-peptide isomerase activity in aqueous extracts of papaya fruit.

    Science.gov (United States)

    Arakawa, Kensuke; Koh, Jennifer M S; Crossett, Ben; Torres, Allan M; Kuchel, Philip W

    2012-09-01

    Peptide isomerase catalyses the post-translational isomerisation of the L: - to the D: -form of an amino acid residue around the N/C-termini of substrate peptides. To date, some peptide isomerases have been found in a limited number of animal secretions and cells. We show here that papaya extracts have weak peptide isomerase activity. The activity was detected in each 30-100 kDa fraction of the flesh and the seed extracts of unripe and ripe papaya fruit. The definitive activity was confirmed in the ripe papaya extracts, but even then it was much less active than that of the other peptide isomerases previously reported. The activity was markedly inhibited by methanol, and partly so by amastatin and diethyl pyrocarbonate. This is the first report of peptide isomerase activity in a plant and suggests that perhaps every living organism may have some peptide isomerase activity.

  2. Pathogenicity of three entomopathogenic fungi, Metarhizium anisopliae, Beauveria bassiana, and Paecilomyces lilacinus, to Tetranychus kanzawai infesting papaya seedlings

    Directory of Open Access Journals (Sweden)

    Yayan Sanjaya

    2016-09-01

    Full Text Available Tetranychus kanzawai is an economically important polyphagous mite species in East and South Asia. In the Philippines, it commonly infests cassava and papaya plants. The mites attack and severely damage the older leaves of papaya trees as well as seedlings. Its serious damage causes the leaves to dry up, thus, reducing the photosynthetic activity of the plant. Three entomopathogenic fungal isolates were tested on mites under greenhouse conditions using treated papaya seedlings following a completely randomized design. The mites tested were examined under a dissecting scope to determine the causal agent and to confirm mortality. The LT50 of Metarhizium anisopliae, Beauveria bassiana and Paecilomyces lilacinus on T. kanzawai were estimated. Our results indicate that among these entomopathogenic fungi, the Metarhizium anisopliae Ma6 isolate (LT50= 3.00 days has potentiality for the control of T. kanzawai.

  3. La solidez de la economía chilena a la luz de las reformas estructurales: 1970-1997

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    Ángeles SÁNCHEZ DÍEZ

    2009-11-01

    Full Text Available RESUMEN: En el presente artículo se recogen las principales reformas estructurales que han tenido lugar en Chile desde 1970 y que constituyen la base de la solidez actual de dicha economía latinoamericana. Se propone una división en períodos en la que se distingue la época del gobierno de la Unidad Popular, el gobierno de la Junta Militar y la transición a la democracia para evaluar los resultados económicos que se registraron a la luz de las distintas estrategias de desarrollo. Las principales reformas que se dieron en el periodo 1970-1973 bajo la "vía chilena hacia el socialismo" fueron la creación del Área de Propiedad social, la profundización de la Reforma Agraria y la nacionalización de la Gran Minería Durante el monetarismo ortodoxo (1974-1982 destacan como principales reformas la liberalización y desregulación de los mercados, la l a ronda de privatizaciones, la apertura comercial, la liberalización financiera y las "siete modernizaciones", todas ellas respaldadas por la ortodoxia de la denominada Escuela de Chicago. Tras la profunda crisis de 1982 que afectó a todo el continente latinoamericano fue necesario replantear el modelo y se introdujeron distintas medidas de carácter neoliberal que consiguieron que fuera posible aprovechar los beneficios de las reformas realizadas con anterioridad. La transición a la democracia constituye un reto para la sociedad chilena, en tanto que contando con las bases de una economía sólida deben incorporarse la visión social y política para que el crecimiento se convierta en desarrollo. En este sentido toma importancia la última estrategia de la CEPAL de "Transformación productiva con equidad".ABSTRACT: In this paper, the main structural reforms are searched, which happened between 1970 and 1995. These are the base of the actual economic framework. The different considered periods are: the "Unidad Popular" government, the military government and the democratic transition. Finally

  4. CARACTERIZACIÓN DE COMPOST A BASE DE ESPINILLO EN RELACIÓN A LA NORMA CHILENA No2880

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    Ricardo Tighe-Neira

    2014-01-01

    Full Text Available Caracterización de compost a base de espinillo en relación a la Norma Chilena No2880. El objetivo de este estudio fue caracterizar el compost a base de espinillo (Ulex europaeus L. y contrastar sus características físico-químicas con las de la Norma Chilena de compost (NCh 2880. El ensayo se montó en el Centro Experimental Pillanlelbún, Temuco, Chile, entre diciembre 2010 y abril de 2011. Se empleó un diseño factorial de 2×2, los factores fueron: mezcla y acelerador de compostaje (AC, ambos en dos niveles. Cuatro tratamientos: T1 U. europaeus (100% sin AC, T2 U. europaeus (78% + estiércol de bovino (22% sin AC, T3 U. europaeus (100% con AC y T4 U. europaeus (78% + estiércol de bovino (22% con AC. Se evaluó el contenido de materia orgánica (MO%, C orgánico total (%, relación C:N, N total (%, humedad (Ho%, tamaño partículas (TP%, toxicidad (% germinación de Raphanus sativus L., pH, densidad aparente (DAP kg/m3, conductividad eléctrica (CE dS/m y germinación de malezas (No de propágulos. Los tratamientos U. europaeus (100% sin AC y con AC presentaron mayor %MO, %N total y relación C:N que T2 y T4. A la vez, U. europaeus (100% sin AC, presentó un menor %C y T2 menor pH en comparación al resto de los tratamientos. Las demás variables evaluadas no presentaron diferencias significativas. Todas cumplieron con la NCh 2880, excepto toxicidad en el tratamiento con solo U. europaeus, y la CE en todos los tratamientos. Se debe reducir la CE para poder optar a su certificación por la NCh 2880.

  5. New Cultivars Derived from Crosses between Commercial Cultivar and a Wild Population of Papaya Rescued at Its Center of Origin

    OpenAIRE

    Mariela Vázquez Calderón; Manuel Jesús Zavala León; Fernando Amilcar Contreras Martín; Francisco Espadas y Gil; Abelardo Navarrete Yabur; Lorenzo Felipe Sánchez Teyer; Jorge M. Santamaría

    2014-01-01

    In order to generate new varieties, this study focused on the rescue and use of landraces and wild Carica papaya L. populations located at southern Yucatan, Mexico, to cross them with a commercial papaya cultivar (Maradol). In the cross L7 × M22, The native parent line L7 was used as the receiver parent while the commercial Maradol (M22) was used as the donor parent, seeking to generate genotypes with improved productivity and reduced plant height. Cluster analysis and principal components an...

  6. Effect of irradiation on the biochemical and organoleptic changes during the ripening of papaya and mango fruits

    Energy Technology Data Exchange (ETDEWEB)

    Lacroix, M.; Bernard, L.; Jobin, M.; Milot, S.; Gagnon, M. (Centre d' Irradiation du Canada, Laval, Quebec (Canada))

    1990-01-01

    Papaya and mango rot caused by fungi is a major problem during storage and marketing. Gamma irradiation treatment was used to determine its effect on the quality of papayas and mangoes irradiated at 0,5 to 0,95 kGy. The level of respiration, soluble solids, texture, vitamin C and the sensorial evaluation were effectuated. The results indicate that irradiation treatment reduces significantly (p{le}0,001) weakens the texture of mangoes. The content of soluble solids and vitamin C are not significantly affected by the irradiation. The sensory evaluation indicates that up to 0,95 kGy the sensorial quality is not changed. (author).

  7. AKTIVITAS ANTIBAKTERI GETAH PEPAYA KERING TERHADAP Staphylococcus aureus PADA DANGKE [Antibacterial Activity of Dried Papaya Latex toward Staphylococcus aureus in Dangke

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    Rifah Hestyani Arum

    2014-06-01

    Full Text Available Dangke is a traditional milk curd product, made by coagulation of milk using fresh papaya latex. This product is usually kept at room temperature (27-30ºC until consumption. Dried papaya latex was used in this study to produce dangke, and its effect to S. aureus was determined by direct contact in TSB and dangke. Fresh papaya latex was dried using vacuum oven at 50-55ºC for 22 hours. Dried papaya latex at a concentration of 2.7x10-3 g/100 mL could reduce S. aureus approximately 1 log CFU/mL in TSB after 24 hours. Dried papaya latex and papain could maintain the S. aureus number in dangke within 24 hours storage at room temperature. The antibacterial activity of non-proteolytic compound of papaya latex, i.e ethanolic extract of papaya latex was determined by macrodilution method, resulted an the MIC90 of 8 mg/mL. The cell membrane leakage after exposure was detected by measuring the optical density of bacterial supernatant at 260 nm. The result showed that exposure to increasing antibacterial concentration resulted in increasing of optical density of S. aureus supernatant, indicating that the antibacterial caused the S. aureus membrane leakage. Fluorescence microscopy imaging showed that S. aureus exposure to antibacterial caused membrane leakage thus gave Propidium Iodide (PI chance to penetrate into the cell, as indicated by changing of fluorescence color from green to red.

  8. Morphological study of bone marrow to assess the effects of lead acetate on haemopoiesis and aplasia and the ameliorating role of Carica papaya extract.

    Science.gov (United States)

    Tham, Ching S; Chakravarthi, Srikumar; Haleagrahara, Nagaraja; DE Alwis, Ranjit

    2013-02-01

    Lead causes damage to the body by inducing oxidative stress. The sites of damage include the bone marrow, where marrow hypoplasia and osteosclerosis may be observed. Leaves of Carica papaya, which have antioxidant and haemopoietic properties, were tested against the effect of lead acetate in experimental rats. The rats were divided into 8 groups; control, lead acetate only, Carica papaya (50 mg and 200 mg), post-treatment with Carica papaya (50 mg and 200 mg) following lead acetate administration and pre-treatment with Carica papaya (50 mg and 200 mg) followed by lead acetate administration. The substances were administered for 14 days. The effects were evaluated by measuring protein carbonyl content (PCC) and glutathione content (GC) in the bone marrow. Histological changes in the bone marrow were also observed. The results showed that Carica papaya induced a significant reduction in the PCC activity and significantly increased the GC in the bone marrow. Carica papaya also improved the histology of the bone marrow compared with that of the lead acetate-treated group. In summary, Carica papaya was effective against the oxidative damage caused by lead acetate in the bone marrow and had a stimulatory effect on haemopoiesis.

  9. Papaya seedlings growth using a low-cost, automatic watering controller

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    Gustavo Cardoso de Oliveira Dias

    2013-06-01

    Full Text Available The study assessed growth and physiological parameters of 'Sunrise Golden' and 'Tainung 01' papaya seedlings grown in 280mL plastic tubes and watered using a low-cost automatic irrigation system adjusted to operate at substrate water tension for starting irrigation (STI of 3.0, 6.0 or 9.0 kPa. The water depths applied by the dripping system and drainage were monitored during germination and seedling growth. Germination, emergence velocity index (EVI, leaf area, plant height, shoot and root dry weight, stomatal conductance, relative water content (RWC and relative chlorophyll content (RCC were evaluated. Soil nutrient levels were determined by electrical conductivity (EC. Water use efficiency (WUE corresponded to the ratio of plant dry mass to depth of water applied. STI settings did not affect papaya germination or EVI. System configuration to 3.0 and 6.0 kPa STI exhibited the highest drainage and lowest EC and RCC, indicating soil nutrient loss and plant nutrient deficiency. Drainage was greater in tubes planted with the 'Tainung 01' variety, which developed smaller root systems and lower stomatal conductance than 'Sunrise Golden' seedlings. The highest values for shoot dry weight and WEU were obtained at 6.0 kPa STI for 'Sunrise Golden' (0.62 g and 0.69 g L-1 and at 9.0 kPa in 'Tainung 01' (0.35 g and 0.82 g L-1. RWC at 9.0 kPa STI was lower than at 3.0 kPa in both varieties. The results indicate that the low-cost technology developed for irrigation automation is promising. Even so, new studies are needed to evaluate low-flow irrigation systems as well as the nutrient and water needs of different papaya varieties.

  10. Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditions.

    Science.gov (United States)

    Zhu, Xiaoyang; Li, Xueping; Chen, Weixin; Chen, Jianye; Lu, Wangjin; Chen, Lei; Fu, Danwen

    2012-01-01

    Real-time reverse transcription PCR (RT-qPCR) is a preferred method for rapid and accurate quantification of gene expression studies. Appropriate application of RT-qPCR requires accurate normalization though the use of reference genes. As no single reference gene is universally suitable for all experiments, thus reference gene(s) validation under different experimental conditions is crucial for RT-qPCR analysis. To date, only a few studies on reference genes have been done in other plants but none in papaya. In the present work, we selected 21 candidate reference genes, and evaluated their expression stability in 246 papaya fruit samples using three algorithms, geNorm, NormFinder and RefFinder. The samples consisted of 13 sets collected under different experimental conditions, including various tissues, different storage temperatures, different cultivars, developmental stages, postharvest ripening, modified atmosphere packaging, 1-methylcyclopropene (1-MCP) treatment, hot water treatment, biotic stress and hormone treatment. Our results demonstrated that expression stability varied greatly between reference genes and that different suitable reference gene(s) or combination of reference genes for normalization should be validated according to the experimental conditions. In general, the internal reference genes EIF (Eukaryotic initiation factor 4A), TBP1 (TATA binding protein 1) and TBP2 (TATA binding protein 2) genes had a good performance under most experimental conditions, whereas the most widely present used reference genes, ACTIN (Actin 2), 18S rRNA (18S ribosomal RNA) and GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) were not suitable in many experimental conditions. In addition, two commonly used programs, geNorm and Normfinder, were proved sufficient for the validation. This work provides the first systematic analysis for the selection of superior reference genes for accurate transcript normalization in papaya under different experimental conditions.

  11. Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditions.

    Directory of Open Access Journals (Sweden)

    Xiaoyang Zhu

    Full Text Available Real-time reverse transcription PCR (RT-qPCR is a preferred method for rapid and accurate quantification of gene expression studies. Appropriate application of RT-qPCR requires accurate normalization though the use of reference genes. As no single reference gene is universally suitable for all experiments, thus reference gene(s validation under different experimental conditions is crucial for RT-qPCR analysis. To date, only a few studies on reference genes have been done in other plants but none in papaya. In the present work, we selected 21 candidate reference genes, and evaluated their expression stability in 246 papaya fruit samples using three algorithms, geNorm, NormFinder and RefFinder. The samples consisted of 13 sets collected under different experimental conditions, including various tissues, different storage temperatures, different cultivars, developmental stages, postharvest ripening, modified atmosphere packaging, 1-methylcyclopropene (1-MCP treatment, hot water treatment, biotic stress and hormone treatment. Our results demonstrated that expression stability varied greatly between reference genes and that different suitable reference gene(s or combination of reference genes for normalization should be validated according to the experimental conditions. In general, the internal reference genes EIF (Eukaryotic initiation factor 4A, TBP1 (TATA binding protein 1 and TBP2 (TATA binding protein 2 genes had a good performance under most experimental conditions, whereas the most widely present used reference genes, ACTIN (Actin 2, 18S rRNA (18S ribosomal RNA and GAPDH (Glyceraldehyde-3-phosphate dehydrogenase were not suitable in many experimental conditions. In addition, two commonly used programs, geNorm and Normfinder, were proved sufficient for the validation. This work provides the first systematic analysis for the selection of superior reference genes for accurate transcript normalization in papaya under different experimental

  12. Informativeness of minisatellite and microsatellite markers for genetic analysis in papaya.

    Science.gov (United States)

    Oliveira, G A F; Dantas, J L L; Oliveira, E J

    2015-10-01

    The objective of this study was to evaluate information on minisatellite and microsatellite markers in papaya (Carica papaya L.). Forty minisatellites and 91 microsatellites were used for genotyping 24 papaya accessions. Estimates of genetic diversity, genetic linkage and analyses of population structure were compared. A lower average number of alleles per locus was observed in minisatellites (3.10) compared with microsatellites (3.57), although the minisatellites showed rarer alleles (18.54 %) compared with microsatellite (13.85 %). Greater expected (He = 0.52) and observed (Ho = 0.16) heterozygosity was observed in the microsatellites compared with minisatellites (He = 0.42 and Ho = 0.11), possibly due to the high number of hermaphroditic accessions, resulting in high rates of self-fertilization. The polymorphic information content and Shannon-Wiener diversity were also higher for microsatellites (from 0.47 to 1.10, respectively) compared with minisatellite (0.38 and 0.85, respectively). The probability of paternity exclusion was high for both markers (>0.999), and the combined probability of identity was from 1.65(-13) to 4.33(-38) for mini- and micro-satellites, respectively, which indicates that both types of markers are ideal for genetic analysis. The Bayesian analysis indicated the formation of two groups (K = 2) for both markers, although the minisatellites indicated a substructure (K = 4). A greater number of accessions with a low probability of assignment to specific groups were observed for microsatellites. Collectively, the results indicated higher informativeness of microsatellites. However, the lower informative power of minisatellites may be offset by the use of larger number of loci. Furthermore, minisatellites are subject to less error in genotyping because there is greater power to detect genotyping systems when larger motifs are used.

  13. Identification and biochemical characterization of a GDSL-motif carboxylester hydrolase from Carica papaya latex.

    Science.gov (United States)

    Abdelkafi, Slim; Ogata, Hiroyuki; Barouh, Nathalie; Fouquet, Benjamin; Lebrun, Régine; Pina, Michel; Scheirlinckx, Frantz; Villeneuve, Pierre; Carrière, Frédéric

    2009-11-01

    An esterase (CpEst) showing high specific activities on tributyrin and short chain vinyl esters was obtained from Carica papaya latex after an extraction step with zwitterionic detergent and sonication, followed by gel filtration chromatography. Although the protein could not be purified to complete homogeneity due to its presence in high molecular mass aggregates, a major protein band with an apparent molecular mass of 41 kDa was obtained by SDS-PAGE. This material was digested with trypsin and the amino acid sequences of the tryptic peptides were determined by LC/ESI/MS/MS. These sequences were used to identify a partial cDNA (679 bp) from expressed sequence tags (ESTs) of C. papaya. Based upon EST sequences, a full-length gene was identified in the genome of C. papaya, with an open reading frame of 1029 bp encoding a protein of 343 amino acid residues, with a theoretical molecular mass of 38 kDa. From sequence analysis, CpEst was identified as a GDSL-motif carboxylester hydrolase belonging to the SGNH protein family and four potential N-glycosylation sites were identified. The putative catalytic triad was localised (Ser(35)-Asp(307)-His(310)) with the nucleophile serine being part of the GDSL-motif. A 3D-model of CpEst was built from known X-ray structures and sequence alignments and the catalytic triad was found to be exposed at the surface of the molecule, thus confirming the results of CpEst inhibition by tetrahydrolipstatin suggesting a direct accessibility of the inhibitor to the active site.

  14. ANTICANCER EFFECTS OF CARICA PAPAYA IN EXPERIMENTAL INDUCED MAMMARY TUMORS IN RATS

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    Gurudatta M, Deshmukh YA, Naikwadi A A

    2015-07-01

    Full Text Available Objective: To evaluate the anticancer effect of Carica papaya in DMBA induced mammary tumors in rats. Methods: Wistar rats were divided in to five groups (n=6, Group-I (Normal control administered vehicle olive oil, Group-II, Group-III ,Group-IV and V induced mammary tumors by administering single dose of DMBA (7,12 Dimethyl benz(Aanthracene orally 65 mg/kg. Group-III was administered aqueous leaf extract of Carica papaya (ALQECP in a dose of 200 mg/kg body wt for a period of 3 months, group-IV has given ALQECP 200 mg/kg body wt for a period of 21 days post 3 months of tumor induction, group-V rats were administered a small dose of Carica papaya extract intra tumor locally in the region of tumor. Results: Values of CA15-3 were increased in group-II rats (tumor control significantly, whereas in group-III (prevention group the levels of CA15-3 were found to be reduced substantially and the P value < 0.001. Similarly, CA-15-3 levels were reduced significantly in group-IV (treatment groupand P<0.005. The levels of LDH were seen to be increased in group-II, where as in group-III LDH levels were decreased and P<0.001.similarly group-IV LDH levels also reduced significantly but not to the level of group-III. Conclusion: Among the various markers for the detection of cancer antigen-15(CA15-3 and lactate dehydrogenase (LDH are important biochemical parameters that give a clear understanding of the progression and proliferation of cancer cells. In this study it was found that there is increase in the levels of markers such as CA15-3 and LDH and also the tumor volume in tumor control, these marker levels were decreased by the administration of aqueous leaf extract of Carica papaya in a dose of 200 mg/kg body wt. ALQECP not only prevented the progression of cancer growth but also has significant effect in reducing the both CA15-3 and LDH levels in treatment group.

  15. Evaluation of the composition of Carica papaya L. seed oil extracted with supercritical CO2.

    Science.gov (United States)

    Barroso, Pedro T W; de Carvalho, Pedro P; Rocha, Thiago B; Pessoa, Fernando L P; Azevedo, Debora A; Mendes, Marisa F

    2016-09-01

    Among the most important tropical fruit grown in the world today and in Brazil, papaya occupies a prominent place. Native to tropical America, papaya has spread to several regions of the world, and Brazil accounts for 12.74% of the world production, followed by Mexico, Nigeria and India. The culture reached a harvested area of 441,042 ha and production of 12,420,585 t worldwide. The largest interest in this fruit relies on its main constituent compounds, like vitamins A, B and C, alkaloids (carpaine and pseudocarpaine), proteolytic enzymes (papain and quimiopapain) and benzyl isothiocyanate, more known as BITC, which has anthelmintic activity. Because of that, the present work has as objective the evaluation of the efficiency and composition of the oil extracted from Carica papaya L. seeds with supercritical carbon dioxide. The experiments were performed in a unit containing mainly a high-pressure pump and a stainless steel extractor with 42 mL of volume. The sampling was performed at each 20 min until the saturation of the process. About 6.5 g of sample were fed for each experiment done at 40, 60 and 80 °C under the pressures of 100, 150 and 200 bar. Samples of the Carica papaya L. fruit were acquired in a popular market and free for personal use intended for the study. After collection, the seeds were crushed with the help of a pestle, and dried at 60 °C for 60 min. For each operational condition, the extraction curves were constructed relating cumulative mass of oil extracted in function of the operational time. The better efficiencies were found at 40 °C and 200 bar (1.33%) followed by 80 °C and 200 bar (2.56%). Gas chromatography and NMR analysis could identify an insecticide component (BITC) that enables new applications of this residue in pharmaceutical and chemical industries.

  16. Effects of the methanolic seeds extract of Carica Papaya on plasmodium Berghei infected mice

    Institute of Scientific and Technical Information of China (English)

    Amazu LU; Ebong OO; Azikiwe CCA; Unekwe PC; Siminialayi MI; Nwosu PJC; Ezeani MC; Obidiya OS; Ajugwo AO

    2009-01-01

    Objective:The leaves extract of Carica Papaya(C.Papaya)papaya has been shown to possess antimalarial ac-tivity,thus this work aims at finding out if the plants antimalarial activity is present in or extended to the seeds.Methods:The seeds of C.papaya were collected from its fruit,air dried for 5 days and ground into fine powder.80.65 g of the powder was then soaked for 48hours in 300 mL of methanol.Filtration was carried out using Whatman No.1 filter paper.The filtrate was evaporated to dryness by a three-day continuous heating on a hot plate of 30℃.The dry extract yield was scraped out of the Petri dish weighed and refrigerated until re-quired.The percentage extract yield was calculated out from the initial powder weight.A preliminary phyto-chemical study was done by re-dissolving the appropriate amount of the dry extract in distilled water and appro-priate test reagent added.The LD50 of the seeds of C.papaya was carried out using arithmetic method.Swiss albino Mice of both sexes and of average weight of 1 8-25 g were used as animals for antimalarial activity.They were housed in standard animal house,fed on Rats/Mice pellets and had non restricted excess to both feed and water throughout the 60day study period.While the non pregnant female Mice were used as test animals,the male animals were used as malaria parasite donors.Precautions were taken to ensure that all animals in the study groups were free from infection with Eperythrozoon coocoides.The female animals were then divided into three main groups (A-C)of 25 animals per group.Group A was used for malaria suppressive study (early in-fection-day 0-3)and was further subdivided to 5subgroups (a-e)of 5animals per group.Group B was used for malaria curative study (established malaria infection-day 3-7)and was further subdivided to 5subgroups (a-e) of 5animals per group.Group C was used for malaria prophylactic study (repository-4days treatment prior to malaria parasite infection)and was also further subdivided

  17. Imaging of Bacterial and Fungal Cells Using Fluorescent Carbon Dots Prepared from Carica papaya Juice.

    Science.gov (United States)

    Kasibabu, Betha Saineelima B; D'souza, Stephanie L; Jha, Sanjay; Kailasa, Suresh Kumar

    2015-07-01

    In this paper, we have described a simple hydrothermal method for preparation of fluorescent carbon dots (C-dots) using Carica papaya juice as a precursor. The synthesized C-dots show emission peak at 461 nm with a quantum yield of 7.0 %. The biocompatible nature of C-dots was confirmed by a cytotoxicity assay on E. coli. The C-dots were used as fluorescent probes for imaging of bacterial (Bacillus subtilis) and fungal (Aspergillus aculeatus) cells and emitted green and red colors under different excitation wavelengths, which indicates that the C-dots can be used as a promising material for cell imaging.

  18. Crystallization and preliminary X-ray analysis of papaya mosaic virus coat protein.

    Science.gov (United States)

    Zhang, H; Todderud, E; Stubbs, G

    1993-12-05

    Papaya mosaic virus coat protein has been treated with trypsin and a large fragment of the intact protein has been crystallized in space group P3(1)21 or P3(2)21 (unit cell dimensions: a = b = 110 A, c = 237 A). The crystals diffract to 3.5 A resolution. Crystals of the untreated protein have also been grown. The untreated protein crystals diffract to 4 A resolution, but have a large mosaic spread. They have the same space group as the trypsin-treated protein crystals, but a much smaller unit cell (a = b = 72 A, c = 240 A).

  19. The primary structure of papaya mosaic virus coat protein: a revision.

    Science.gov (United States)

    Verde, C; Malorni, A; Parente, A

    1989-12-01

    The presence of an acetyl blocking group at the N-terminus of the coat protein of papaya mosaic virus has been identified by FAB mass spectrometry. Furthermore, we have found that the N-terminal sequence of the protein is four amino-acid residues (AC-Ser-Lys-Ser-Ser-) longer than that previously reported, while Glu instead of Gln is the C-terminal residue. The present paper shows that PMV-protein is made up of 215 amino acid residues, with a molecular mass of 22,960 Da.

  20. Effect of Carica papaya bark extract on oxidative stress parameters in testes of male albino rats

    Directory of Open Access Journals (Sweden)

    T O Kusemiju

    2012-01-01

    Full Text Available Summary: Carica papaya bark has contraceptive benefits when given at a high dose in male rats the mechanism at which it achieves this is yet to be fully understood. Forty male Wistar rats were used for the study. They were randomized into 8 groups Ia, IIa, IIIa, Ib, IIb, IIIb, IV and V respectively (n=5. Groups Ia-IIIa and Ib-IIIb were treated for 4 and 8 weeks with Carica papaya bark extract at doses 50 and 100 mg ml-1day-1 comparing to 2-5 ml distilled water baseline control. Group IV rats were observed for reversibility treated with alternating bark extract and distilled water for 16 weeks (8 weeks each. They were compared to Group V treated with distilled water alone for similar duration. The rats were sacrificed under chloroform anesthesia. The estimated parameters were testes volumes biochemical activities of testicular antioxidant enzymes and non-enzymes [superoxide dismutase (SOD, catalase (CAT, reduced glutathione (GSH and malondialdehyde (MDA] as well as total protein (TP. The extract treated groups (Groups Ia, IIa, Ib & IIb showed a decrease in TP and testicular volume. Also in these groups were significant increases in testicular MDA levels compared to control (Groups IIIa & IIIb. At both durations for the two doses, the extract resulted in a significant decrease in antioxidant enzymes. Animals in the reversibility study group showed values similar to baseline control. In conclusion, Carica papaya leaf extract essentially perturbed the testicular oxidative system this may be responsible for the contraceptive effect seen. Industrial relevance: The contraceptive benefits of aqueous extract of Carica papaya bark in orally treated male rats had been confirmed. This present study was tailored at reviewing variations in the gonadal proteins and stressors index in male rats administered established contraceptive doses of the extract. This would be helpful to industries intending to develop this herbal preparation as a contraceptive adjunct

  1. Evaluation of the composition of Carica papaya L. seed oil extracted with supercritical CO2

    Directory of Open Access Journals (Sweden)

    Pedro T.W. Barroso

    2016-09-01

    Full Text Available Among the most important tropical fruit grown in the world today and in Brazil, papaya occupies a prominent place. Native to tropical America, papaya has spread to several regions of the world, and Brazil accounts for 12.74% of the world production, followed by Mexico, Nigeria and India. The culture reached a harvested area of 441,042 ha and production of 12,420,585 t worldwide. The largest interest in this fruit relies on its main constituent compounds, like vitamins A, B and C, alkaloids (carpaine and pseudocarpaine, proteolytic enzymes (papain and quimiopapain and benzyl isothiocyanate, more known as BITC, which has anthelmintic activity. Because of that, the present work has as objective the evaluation of the efficiency and composition of the oil extracted from Carica papaya L. seeds with supercritical carbon dioxide. The experiments were performed in a unit containing mainly a high-pressure pump and a stainless steel extractor with 42 mL of volume. The sampling was performed at each 20 min until the saturation of the process. About 6.5 g of sample were fed for each experiment done at 40, 60 and 80 °C under the pressures of 100, 150 and 200 bar. Samples of the Carica papaya L. fruit were acquired in a popular market and free for personal use intended for the study. After collection, the seeds were crushed with the help of a pestle, and dried at 60 °C for 60 min. For each operational condition, the extraction curves were constructed relating cumulative mass of oil extracted in function of the operational time. The better efficiencies were found at 40 °C and 200 bar (1.33% followed by 80 °C and 200 bar (2.56%. Gas chromatography and NMR analysis could identify an insecticide component (BITC that enables new applications of this residue in pharmaceutical and chemical industries.

  2. Relative humidity requirements and incubation periods for the Colletotrichum gloeosporioides conidia infection in papaya fruit.

    OpenAIRE

    Durán, Alfredo; Ramírez, Lenín; Mora, Dennis

    2016-01-01

    The research was carried out at the University of Costa Rica's phytopathology laboratory between October 1995 and March 1996. In an initial phase, some creole papaya fruits from Gu.cimo (Limón province) were used. In order to eliminate latent infections, they were washed and immersed in hot water (49° C for 20 minutes). The fruits were then randomly placed in groups of 20, which conformed each treatment. Finally, they were inoculated by spraying with 5.5 ml of a 10.000 Colletotrichum gloeospo...

  3. Isolation And Purification Of Flavonoids From The Leaves Of Locally Produced Carica Papaya

    Directory of Open Access Journals (Sweden)

    Yahaya Mobmi Musa

    2015-08-01

    Full Text Available ABSTRACT The leaves of Carica papaya 150g was defatted with N-Hexane and extracted with Methanol. The N-Hexane exract showed the presence of Flavonoid Saponin Tannin Glycoside Anthraquinone Resin and Steroid while Methanolic extract showed the presence of Flavonoid Saponin and Resins. 6g of the Methanolic extract was chromatographed using Column chromatography over Silica gel of column 200g60-200 mesh and eluted with the solvent mixture of CH2Cl2CH3OH H2O in the ratio of 70301. The yield of the isolated Flavonoid was 0.23.

  4. BARRERAS DE APRENDIZAJE PARA ESTUDIANTES CON DISCAPACIDAD EN UNA UNIVERSIDAD CHILENA. DEMANDAS ESTUDIANTILES – DESAFÍOS INSTITUCIONALES

    Directory of Open Access Journals (Sweden)

    Angélica Corrales Huenul

    2016-01-01

    Full Text Available El presente artículo expone los resultados de una investigación sobre estudiantes con discapacidad de una universidad chilena. El objetivo del estudio es determinar las barreras para el aprendizaje y la participación que enfrentan estos estudiantes y, de esta manera, determinar los apoyos educativos requeridos para que estos puedan realizar sus estudios de manera equitativa en relación con la población estudiantil universitaria. En el plano metodológico, la investigación fue definida como descriptiva y transversal y la principal técnica de recolección de datos fue el cuestionario. Los resultados indican que las barreras de aprendizaje se desarrollan en torno a tres categorías: infraestructura, proceso de enseñanza-aprendizaje y gestión institucional. Los estudiantes según su situación de discapacidad presentan demandas diferenciadas, por lo que este factor se constituye en un aspecto central para examinar los apoyos educativos solicitados. A partir de los resultados, se analizan los desafíos y se proponen estrategias con el objeto de mejorar las políticas de accesibilidad a la universidad.

  5. Estudiantes con discapacidad en una universidad chilena: desafíos de la inclusión

    Directory of Open Access Journals (Sweden)

    Gabriela Paulina VILLAFAÑE HORMAZÁBAL

    2016-01-01

    Full Text Available El propósito de este artículo es dar cuenta de una investigación acerca de las condiciones de inclusión para los estudiantes con discapacidad en una universidad chilena. La investigación tiene un diseño cuantitativo, descriptivo y transversal. Para la recolección de datos se elaboró una encuesta que fue aplicada a 38 estudiantes con discapacidad. Los principales resultados revelan un alto nivel de retención de los estudiantes, quienes presentan una percepción positiva de su inclusión en la vida universitaria, así como una alta satisfacción con la mayoría de los servicios. Se destaca que siete de cada diez encuestados reconocen haber recibido algún tipo de atención educativa para realizar sus estudios. Sin embargo, existe una falta de articulación entre las iniciativas de la Universidad para favorecer el acceso y permanencia de personas con discapacidad; a esto se agrega la carencia de protocolos y de instancias de capacitación para profesores y administrativos. Se propone que la institución establezca un sistema de gestión que defina objetivos, estrategias y acciones para contribuir a la inclusión de personas con discapacidad.

  6. Relación entre el rendimiento y las estrategias de crecimiento en las sociedades anónimas chilenas

    Directory of Open Access Journals (Sweden)

    Alex Medina Giacomozzi

    2013-01-01

    Full Text Available La relación entre los resultados empresariales y la diversificación o concentración es un tema muy rele- vante, por lo que en este artículo se exponen las evidencias obtenidas al comparar el rendimiento obtenido por 82 sociedades anónimas chilenas, seleccionadas al azar, con su estrategia de concentración o diversi- ficación. La metodología seguida implicó la clasificación de las estrategias mediante una matriz con base en el Código Industrial Internacional Uniforme (CIIU de Chile y la aplicación del método Du Pont para la determinación del resultado. La principal evidencia es que la mayoría son empresas concentradas, pero las sociedades con diversificación relacionada obtienen un mayor rendimiento promedio que las empresas concentradas y que las compa ̃ nías con estrategia de diversificación no relacionada.

  7. Validación chilena del cuestionario de evaluación de apego en el adulto CAMIR

    Directory of Open Access Journals (Sweden)

    Lusmenia Garrido

    2009-01-01

    Full Text Available El objetivo de esta investigación fue examinar la confiabilidad y validez del instrumento de evaluación de apego en el adulto, .Cartes: Modèles Individuels de Relation (CAMIR., en la realidad sociocultural chilena. Este instrumento, basado en la teoría del apego, es un cuestionario de auto-reporte que evalúa los modelos operativos internos en adultos y otras escalas relacionadas al apego. Los participantes fueron 578 sujetos, 204 hombres y 374 mujeres, con edades entre 14 y 80 años. Los sujetos completaron el CAMIR y medidas de vínculo y sintomatología actual, a través del Parental Bonding Instrument (PBI and Outcome Questionnaire OQ-45.2. A través del análisis estadístico de los resultados se evaluaron las características psicométricas del test y se analiz ó la posibilidad de generar normas locales para la prueba. Los resultados confirmaron que el CAMIR es un instrumento confiable y válido en la evaluación de los modelos internos de relación en adultos.

  8. FERTILIZACIÓN ORGÁNICA, MINERAL Y FOLIAR SOBRE EL DESARROLLO Y LA PRODUCCIÓN DE PAPAYA CV. MARADOL

    OpenAIRE

    2003-01-01

    El cultivo de la papaya (Carica papaya L.) cv. Maradol es afectado por factores de origen biótico y abiótico, que repercuten en el vigor, la sanidad, el rendimiento y la apariencia del fruto. El objetivo de la presente investigación fue evaluar el efecto de la fertilización orgánica e inorgánica al suelo y foliar, sobre algunas variables fisiológicas de plantas de papaya, concentración nutrimental en hojas, rendimiento y características de los frutos producidos. Los resultados obtenidos revel...

  9. Low-dose gamma irradiation following hot Water immersion of Papaya (Carica Papaya linn.) fruits provides additional control of postharvest fungal infection to extend shelf life

    DEFF Research Database (Denmark)

    Rashid, M.H.A.; Grout, Brian William Wilson; Continella, A.

    2015-01-01

    Low-dose gamma irradiation (0.08 kGy over 10 min), a level significantly below that required to satisfy the majority of international quarantine regulations, has been employed to provide a significant reduction in visible fungal infection on papaya fruit surfaces. This is appropriate for local an...

  10. Effect of temperature on the development and survival of immature stages of the carambola fruit fly, Bactrocera carambolae, and the Asian papaya fruit fly, Bactrocera papayae, reared on guava diet.

    Science.gov (United States)

    Danjuma, Solomon; Thaochan, Narit; Permkam, Surakrai; Satasook, Chutamas

    2014-01-01

    Members of the Bactrocera dorsalis Hendel (Diptera: Tephritidae) complex constitute well-recognized destructive pests of fruits in peninsular Thailand. The development and survival of immature stages of the carambola fruit fly, Bactrocera carambolae Drew & Hancock, and the Asian papaya fruit fly, Bactrocera papayae Drew & Hancock, were compared at six constant temperatures of 15, 20, 25, 27, 30, and 35°C, 70 ± 5% relative humidity, and a photoperiod of 12:12 (L:D). The objectives were to determine the effect of temperature on the developmental stages for optimizing rearing and to understand the geographical pattern of occurrence of these fruit fly species. A strong and positive linear relationship was observed between temperature and developmental rate of immature stages of B. carambolae. Similarly, a strong and positive linear relationship was observed between temperature and developmental rate of B. papayae. A temperature summation model was used to estimate the lower threshold temperature and the thermal constant. Bactrocera papayae was significantly faster in development and higher in survival and appeared to be better adapted to low temperatures than B. carambolae, as it exhibited the lowest threshold temperatures at all immature stages. The observed differences in response to various temperatures revealed to some extent the impact of temperature on these species' distribution in peninsular Thailand and other parts of the world.

  11. Managing Oriental Fruit Fly, Bactrocera dorsalis (Diptera: Tephritidae), Using Spinosad-Based Protein Bait Sprays in Papaya Orchards in Hawaii

    Science.gov (United States)

    The efficacy of GF-120 Fruit Fly Bait was evaluated as a control of female oriental fruit fly, Bactrocera dorsalis (Hendel) in papaya orchards in Hawaii. Two important components of this study were field sanitation and mass trapping using the male-specific lure methyl eugenol. Three different spray ...

  12. New strains of chickpea chlorotic dwarf virus discovered on diseased papaya and tomato plants in Burkina Faso.

    Science.gov (United States)

    Ouattara, Alassane; Tiendrébéogo, Fidèle; Lefeuvre, Pierre; Hoareau, Murielle; Claverie, Sohini; Traoré, Edgar Valentin; Barro, Nicolas; Traoré, Oumar; Varsani, Arvind; Lett, Jean-Michel

    2017-02-22

    This is the first description of full genome sequences of chickpea chlorotic dwarf virus (CpCDV; genus Mastrevirus; family Geminiviridae) identified in papaya and tomato plants sampled in Burkina Faso. The CpCDV full genome sequences from papaya and tomato share the highest pairwise sequence identity (84% and 93.5%) with Sudanese isolates of the CpCDV-K and CpCDV-M strains, respectively. Based on the strain demarcation threshold (>94% identity) for mastreviruses, we propose two new strains, CpCDV-Q and CpCDV-R, identified in papaya and tomato, respectively. Phylogenetic analysis confirmed that the sequences belong to a distinct clade of the highly diverse population of CpCDVs. Evidence of inter-strain recombination provided more support for the important role of recombination in CpCDV evolution. The discovery of CpCDV on papaya, a previously unsuspected host, raises many questions about the natural and potential host range of this dicot-infecting mastrevirus species that is reported to be emerging worldwide.

  13. Effects of chitosan-based coatings containing peppermint essential oil on the quality of post-harvest papaya fruit

    Science.gov (United States)

    Edible coatings comprised of antimicrobial polymers based on chitosan are promising technologies to preserve post-harvest fruit quality. In this study, we investigated the potential utility of a coating made from chitosan modified by N-acylation with fatty acid to preserve post-harvest papaya qualit...

  14. Resistance to papaya ringspot virus-watermelon strain (PRSV-W) in the desert watermelon Citrullus colocynthis

    Science.gov (United States)

    The bitter desert watermelon (Citrullus colocynthis) is a valuable source for improving disease or pest resistance in watermelon cultivars. The objective of this study was to identify C. colocynthis accessions displaying resistance to the papaya ringspot virus-watermelon strain (PRSV-W) that could ...

  15. Long-term spatial memory and morphological changes in hippocampus of Wistar rats exposed to smoke from Carica papaya leaves

    Institute of Scientific and Technical Information of China (English)

    Aboyeji Lukuman Oyewole; Bamidele Victor Owoyele

    2014-01-01

    Objective:To investigate the effects of smoking of dried leaves of Carica papaya (pawpaw) based on ethnopharmacological information which indicated that smoking of papaya leaves could influence motor performance and learning. Methods:Twenty-four rats were used for the study, and were grouped into four groups. Groups 1 served as the control (not exposed to papaya leaves smoke), while Groups 2, 3 and 4 were exposed to smoke from 6.25 g, 12.50 g and 18.75 g of dry pawpaw leaves respectively in a smoking chamber twice daily for 21 d with each exposure lasting for 3 min. Lastly, hippocampus was harvested in each group for histological study. Results: The results showed that there were significant (P Conclusions: In conclusion, the findings from this study has demonstrated that smoking of papaya leaves has the ability to maintain an intact long-term spatial memory at all doses but retrieving such memory is faster with the low and medium dosages.

  16. Content determination of benzyl glucosinolate and anti-cancer activity of its hydrolysis product inCarica papaya L.

    Institute of Scientific and Technical Information of China (English)

    Ze-You Li; Yong Wang; Wen-Tao Shen; Peng Zhou

    2012-01-01

    Objective:To determine the content of benzyl glucosinolate(BG)in the pulp and the seed and investigate the anti-cancer activity of its hydrolysis product inCarica papaya L.Methods:Determination ofBG was performed on an HypersilBDS C18 column at the wavelength of214 nm with0.1% trifluoroacetic acid (TFA)aqueous solution (A) and 0.1%TFA acetonitrile (B)as the mobile phase. In vitro activity test was adopted with cultured human lung cancerH69 cellin vitro to investigate the inhibition rate of cell proliferation of benzyl isothiocyanate(BITC)againstH69 cell.Results: The pulp has more BG before the maturation of papaya and it nearly disappeared after papaya matured, while the seed containsBG at every stage. Activity test demonstrated that the a higher concentration ofBITC would have better inhibition rate of cell proliferation onH69 cell, and the IC50 was6.5 μmol/L.Conclusions:BG also can be produced in the pulp of papaya and it will be stored in the seed after the fruit has been matured. The hydrolysis product ofBG has certain cancer-prevention anti-cancer activities for human.

  17. How to become a tree without wood--biomechanical analysis of the stem of Carica papaya L.

    Science.gov (United States)

    Kempe, A; Lautenschläger, T; Lange, A; Neinhuis, C

    2014-01-01

    Carica papaya L. does not contain wood, according to the botanical definition of wood as lignified secondary xylem. Despite its parenchymatous secondary xylem, these plants are able to grow up to 10-m high. This is surprising, as wooden structural elements are the ubiquitous strategy for supporting height growth in plants. Proposed possible alternative principles to explain the compensation for lack of wood in C. papaya are turgor pressure of the parenchyma, lignified phloem fibres in the bark, or a combination of the two. Interestingly, lignified tissue comprises only 5-8% of the entire stem mass. Furthermore, the phloem fibres do not form a compact tube enclosing the xylem, but instead form a mesh tubular structure. To investigate the mechanism of papaya's unusually high mechanical strength, a set of mechanical measurements were undertaken on whole stems and tissue sections of secondary phloem and xylem. The structural Young's modulus of mature stems reached 2.5 GPa. Since this is low compared to woody plants, the flexural rigidity of papaya stem construction may mainly be based on a higher second moment of inertia. Additionally, stem turgor pressure was determined indirectly by immersing specimens in sucrose solutions of different osmolalities, followed by mechanical tests; turgor pressure was between 0.82 and 1.25 MPa, indicating that turgor is essential for flexural rigidity of the entire stem.

  18. Genetic differentiation of Colletotrichum gloeosporioides and C. truncatum associated with Anthracnose disease of papaya (Carica papaya L.) and bell pepper (Capsium annuum L.) based on ITS PCR-RFLP fingerprinting.

    Science.gov (United States)

    Maharaj, Ariana; Rampersad, Sephra N

    2012-03-01

    Members of the genus Colletotrichum include some of the most economically important fungal pathogens in the world. Accurate diagnosis is critical to devising disease management strategies. Two species, Colletotrichum gloeosporioides and C. truncatum, are responsible for anthracnose disease in papaya (Carica papaya L.) and bell pepper (Capsicum annuum L.) in Trinidad. The ITS1-5.8S-ITS2 region of 48 Colletotrichum isolates was sequenced, and the ITS PCR products were analyzed by PCR-RFLP analysis. Restriction site polymorphisms generated from 11 restriction enzymes enabled the identification of specific enzymes that were successful in distinguishing between C. gloeosporioides and C. truncatum isolates. Species-specific restriction fragment length polymorphisms generated by the enzymes AluI, HaeIII, PvuII, RsaI, and Sau3A were used to consistently resolve C. gloeosporioides and C. truncatum isolates from papaya. AluI, ApaI, PvuII, RsaI, and SmaI reliably separated isolates of C. gloeosporioides and C. truncatum from bell pepper. PvuII, RsaI, and Sau3A were also capable of distinguishing among the C. gloeosporioides isolates from papaya based on the different restriction patterns that were obtained as a result of intra-specific variation in restriction enzyme recognition sites in the ITS1-5.8S-ITS2 rDNA region. Of all the isolates tested, C. gloeosporioides from papaya also had the highest number of PCR-RFLP haplotypes. Cluster analysis of sequence and PCR-RFLP data demonstrated that all C. gloeosporioides and C. truncatum isolates clustered separately into species-specific clades regardless of host species. Phylograms also revealed consistent topologies which suggested that the genetic distances for PCR-RFLP-generated data were comparable to that of ITS sequence data. ITS PCR-RFLP fingerprinting is a rapid and reliable method to identify and differentiate between Colletotrichum species.

  19. Effect of Prior Heat Stress on the Early Growth of Carica papaya

    Directory of Open Access Journals (Sweden)

    Gideon Olarewaju OKUNLOLA

    2013-12-01

    Full Text Available The experiment was carried out to determine the effects of heat stress on some growth parameters like shoot height, leaf area, fresh weight, dry weight as well as the accumulation of chlorophylls in Carica papaya. Seedlings of C. papaya were exposed to prior heat stress at 40 °C. A group of plants was placed in a Gallenkamp oven for four hours; another group of plants was placed in the oven for eight hours while the third group of plants was placed in a dark cupboard for the period of eight hours. Sampling was carried out at weekly intervals starting from seven days after treatment. Plants were randomly picked from each of the three treatments. Three replicates were used for each parameter. The results obtained from the study showed that there was an increment in the shoot height, leaf area, fresh weight and dry weight from the beginning to the end of the experimental period. However, the accumulation of chlorophylls did not follow a particular pattern. The analysis of variance carried out on the data obtained showed that heat stress had a significant effect on the petiole length, shoot height, leaf length, leaf width, leaf area, fresh weight and dry weight. Heat stress, however, did not produce a significant effect on the accumulation of chlorophylls a and b and total chlorophyll.

  20. The Aqueous Seed Extract Of Carica papaya Linn. Prevents Carbon Tetrachloride Induced Hepatotoxicity In Rats

    Directory of Open Access Journals (Sweden)

    A A Adeneye

    2009-06-01

    Full Text Available Summary: Carica papaya Linn. is known to have a versatile application in African folkloric medicine. In the current study, the dose-dependent (100 – 400 mg/kg/day/oral route and time-course protective effects of the 400 mg/kg/oral route of the aqueous seed extract of unripe and mature Carica papaya fruit (CPE were investigated in carbon tetrachloride (CCl4 hepatotoxic rats for 72 hours. Results showed the extract to cause significant (p<0.05, p<0.001, dose related attenuation in the elevation of serum liver enzyme markers of acute hepatocellular injury (ALT, AST, serum lipids (TG, TC, HDL-c, LDL-c and VLDL-c and serum proteins (TP and ALB. Maximum hepatoprotection was offered at an oral dose of 400 mg/kg/day of the extract. The biochemical results obtained were corroborated by improvements in the CCl4-induced hepatic histological changes. In addition, maximum hepatoprotection was offered at the 400 mg/kg of CPE for up to 3 hours post-CCl4 induction. In conclusion, the results obtained in the current study justify the folkloric application of CPE in the treatment of drug-related hepatic injury. Industrial relevance: Results of the current study provide some scientific information to develop safe and effective products such as food supplements, dietary supplements, etc. that could be useful in the clinical management of patients with drug related hepatic disorders.

  1. Carica papaya lipase: a naturally immobilized enzyme with interesting biochemical properties.

    Science.gov (United States)

    Abdelkafi, Slim; Barouh, Nathalie; Fouquet, Benjamin; Fendri, Imen; Pina, Michel; Scheirlinckx, Frantz; Villeneuve, Pierre; Carrière, Frédéric

    2011-03-01

    Triacylglycerol (TAG) lipases have been thoroughly characterized in mammals and microorganisms, whereas very little is known about plant TAG lipases. The lipolytic activity occurring in all the laticies is known to be associated with sedimentable particles, and all attempts to solubilize the lipolytic activity of Carica papaya latex have been unsuccessful so far. However, some of the biochemical properties of the lipase from Carica papaya latex (CPL) were determined from the insoluble fraction of the latex. The activity was optimum at a temperature of 37°C and a pH of 9.0, and the specific activities of CPL were found to be 2,000 ± 185 and 256 ± 8 U/g when tributyrin and olive oil were used as substrates, respectively. CPL was found to be active in the absence of any detergent, whereas many lipases require detergent to prevent the occurrence of interfacial denaturation. CPL was inactive in the presence of micellar concentrations of Triton X-100, sodium dodecyl sulfate (SDS) and tetradecyl trimethylammonium bromide (TTAB), and still showed high levels of activity in the presence of sodium taurodeoxycholate (NaTDC) and the zwitterionic Chaps detergent. The effects of various proteases on the lipolytic activity of CPL were studied, and CPL was found to be resistant to treatment with various enzymes, except in the presence of trypsin. All these properties suggest that CPL may be a good candidate for various biotechnological applications.

  2. Control of papaya fruits anthracnose by essential oil of Ricinus communis

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    César Luis Siqueira Júnior

    2012-02-01

    Full Text Available The aim of this work was to investigate the potential of castor oil for the control of papaya diseases caused by the fungus Colletotrichum gloeosporioides and the bacterium Pseudomonas caricapapayae. The treatment with 1% castor oil did not significantly affect the fungal growth. The effectiveness of castor oil for the control of anthracnose was shown when 5% and 10% (v/v were used in the assays resulting in reduced mycelial growth. Fungal sporulation was strongly inhibited at 10% (v/v concentration of essential oil. The studies with the fresh fruits treated with 5% (v/v castor oil in aqueous emulsions resulted in effective reduction of pathogen spread in these fruits. No lesion was found in the fruits treated with oil, when compared to the control fruits. Castor oil showed no effect against the P. caricapapayae when tested in vitro. These results suggested the potential use of the castor bean essential oil and its fatty acids constituents for the control of anthracnose in papaya fruits.

  3. Prediction of genetic gain from selection indices for disease resistance in papaya hybrids

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    Marcelo Vivas

    2012-12-01

    Full Text Available In order to select superior hybrids for the concentration of favorable alleles for resistance to papaya black spot, powdery mildew and phoma spot, 67 hybrids were evaluated in two seasons, in 2007, in a randomized block design with two replications. Genetic gains were estimated from the selection indices of Smith & Hazel, Pesek & Baker, Williams, Mulamba & Mock, with selection intensity of 22.39%, corresponding to 15 hybrids. The index of Mulamba & Mock showed gains more suitable for the five traits assessed when it was used the criterion of economic weight tentatively assigned. Together, severity of black spot on leaves and on fruits, characteristics considered most relevant to the selection of resistant materials, expressed percentage gain of -44.15%. In addition, there were gains for other characteristics, with negative predicted selective percentage gain. The results showed that the index of Mulamba & Mock is the most efficient procedure for simultaneous selection of papaya hybrid resistant to black spot, powdery mildew and phoma spot.

  4. Oxidative Stress in Patients with Alzheimer's Disease: Effect of Extracts of Fermented Papaya Powder

    Science.gov (United States)

    Barbagallo, Mario; Marotta, Francesco; Dominguez, Ligia J.

    2015-01-01

    Brain tissue is particularly susceptible to oxidative stress (OS). Increased production of reactive oxygen species (ROS), reduced antioxidant systems, and decreased efficiency in repairing mechanisms have been linked to Alzheimer's disease (AD). Postmortem studies in AD patients' brains have shown oxidative damage markers (i.e., lipid peroxidation, protein oxidative damage, and glycoxidation). Fermented papaya (FPP, a product of Carica papaya Linn fermentation with yeast) is a nutraceutical supplement with favorable effects on immunological, hematological, inflammatory, and OS parameters in chronic/degenerative diseases. We studied 40 patients (age 78.2 ± 1.1 years), 28 AD patients, and 12 controls. Urinary 8-OHdG was measured to assess OS. Twenty AD patients were supplemented with FPP (Immunage, 4.5 grams/day) for 6 months, while controls did not receive any treatment. At baseline, 8-OHdG was significantly higher in patients with AD versus controls (13.7 ± 1.61 ng/mL versus 1.6 ± 0.12 ng/mL, P < 0.01). In AD patients FPP significantly decreased 8-OHdG (14.1 ± 1.7 ng/mL to 8.45 ± 1.1 ng/mL, P < 0.01), with no significant changes in controls. AD is associated with increased OS, and FPP may be helpful to counteract excessive ROS in AD patients. PMID:25944987

  5. On the evolution and molecular epidemiology of the potyvirus Papaya ringspot virus.

    Science.gov (United States)

    Bateson, Marion F; Lines, Rosemarie E; Revill, Peter; Chaleeprom, Worawan; Ha, Cuong V; Gibbs, Adrian J; Dale, James L

    2002-10-01

    The potyvirus Papaya ringspot virus (PRSV) is found throughout the tropics and subtropics. Its P biotype is a devastating pathogen of papaya crops and its W biotype of cucurbits. PRSV-P is thought to arise by mutation from PRSV-W. However, the relative impact of mutation and movement on the structure of PRSV populations is not well characterized. To investigate this, we have determined the coat protein sequences of isolates of both biotypes of PRSV from Vietnam (50), Thailand (13), India (1) and the Philippines (1), and analysed them together with 28 PRSV sequences already published, so that we can better understand the molecular epidemiology and evolution of PRSV. In Thailand, variation was greater among PRSV-W isolates (mean nucleotide divergence 7.6%) than PRSV-P isolates (mean 2.6%), but in Vietnamese populations the P and W biotypes were more but similarly diverse. Phylogenetic analyses of PRSV also involving its closest known relative, Moroccan watermelon mosaic virus, indicate that PRSV may have originated in Asia, particularly in the Indian subcontinent, as PRSV populations there are most diverse and hence have probably been present longest. Our analyses show that mutation, together with local and long-distance movement, contributes to population variation, and also confirms an earlier conclusion that populations of the PRSV-P biotype have evolved on several occasions from PRSV-W populations.

  6. Production of fatty acid butyl esters using the low cost naturally immobilized Carica papaya lipase.

    Science.gov (United States)

    Su, Erzheng; Wei, Dongzhi

    2014-07-01

    In this work, the low cost naturally immobilized Carica papaya lipase (CPL) was investigated for production of fatty acid butyl esters (FABE) to fulfill the aim of reducing the lipase cost in the enzymatic butyl-biodiesel process. The CPL showed specificities to different alcohol acyl acceptors. Alcohols with more than three carbon atoms did not have negative effects on the CPL activity. The CPL catalyzed butanolysis for FABE production was systematically investigated. The reaction solvent, alcohol/oil molar ratio, enzyme amount, reaction temperature, and water activity all affected the butanolysis process. Under the optimized conditions, the highest conversion of 96% could be attained in 24 h. These optimal conditions were further applied to CPL catalyzed butanolysis of other vegetable oils. All of them showed very high conversion. The CPL packed-bed reactor was further developed, and could be operated continuously for more than 150 h. All of these results showed that the low cost Carica papaya lipase can be used as a promising lipase for biodiesel production.

  7. Identification of miRNAs and miRNA-mediated regulatory pathways in Carica papaya.

    Science.gov (United States)

    Liang, Gang; Li, Yang; He, Hua; Wang, Fang; Yu, Diqiu

    2013-10-01

    Plant microRNAs (miRNAs) post-transcriptionally regulate target gene expression to modulate growth and development and biotic and abiotic stress responses. By analyzing small RNA deep sequencing data in combination with the genome sequence, we identified 75 conserved miRNAs and 11 novel miRNAs. Their target genes were also predicted. For most conserved miRNAs, the miRNA-target pairs were conserved across plant species. In addition to these conserved miRNA-target pairs, we also identified some papaya-specific miRNA-target regulatory pathways. Both miR168 and miR530 target the Argonaute 1 gene, indicating a second autoregulatory mechanism for miRNA regulation. A non-conserved miRNA was mapped within an intron of Dicer-like 1 (DCL1), suggesting a conserved homeostatic autoregulatory mechanism for DCL1 expression. A 21-nt miRNA triggers secondary siRNA production from its target genes, nucleotide-binding site leucine-rich repeat protein genes. Certain phased-miRNAs were processed from their conserved miRNA precursors, indicating a putative miRNA evolution mechanism. In addition, we identified a Carica papaya-specific miRNA that targets an ethylene receptor gene, implying its function in the ethylene signaling pathway. This work will also advance our understanding of miRNA functions and evolution in plants.

  8. Germination and biochemical changes in ‘Formosa’ papaya seeds treated with plant hormones

    Directory of Open Access Journals (Sweden)

    Rafael Fonsêca Zanotti

    2014-04-01

    Full Text Available This study aimed to investigate the effects of growth regulators on germination rates and biochemical compound concentrations in Carica papaya L. seeds (‘Formosa’ group. The seeds were harvested from fruits at maturation stages 3 and 5 (50 and 75% yellow fruit skin, respectively. The effects of 2-chloroethylphosphonic acid (CEPA, KNO3 and gibberellic acid (GA3on seed germination, germination index speed, soluble sugars, starch, lipids, soluble proteins and total proteins of the papaya seeds were evaluated. The seeds from stage 5 showed a higher rate of germination 30 days after sowing than did the seeds from stage 3. Treatment with CEPA decreased seed germination, apparently due to decreased starch mobilization; the opposite response was observed following KNO3 treatment. GA3, alone or in combination with KNO3, stimulated an increase in lipid mobilization. In general, with the exception of CEPA, all growth regulators tested were effective in overcoming seed dormancy, and KNO3 was the most effective. The seeds from stage 3 fruits treated with KNO3 or KNO3 + GA3 had higher rates of germination at 14 days.

  9. Nutritional status and fruit production of Carica papaya as a function of coated and conventional urea

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    Gabriel B. da Silva Júnior

    2016-04-01

    Full Text Available ABSTRACT As a strategy to minimize N losses in the soil, mineral N sources, such as polymer-coated urea, have been studied as possibility to increase the synchronization of N release by the fertilizer and its absorption by plants. Thus, this study aimed to evaluate the contents of macronutrients and the production of Formosa papaya as a function of sources and doses of N fertilizer applied as top-dressing in the region of Bom Jesus-PI, Brazil. The treatments were arranged in a 2 x 4 factorial scheme corresponding to N sources (coated urea and conventional urea and N doses (350, 440, 530 and 620 g plant-1 of N, with four replicates and four plants per plot. The contents of macronutrients in the leaf dry matter and fruit production were evaluated. The sources and doses of top-dressing N fertilization incremented the leaf contents of macronutrients and the production of Formosa papaya hybrid Caliman 01. Under the experimental conditions and based on the macronutrient contents considered as adequate for crop nutrition, associated with maximum fruit production (8.08 kg plant-1, the supply of 525 g of N plant-1 is recommended in the form of coated urea.

  10. Comparison of methods for classification of the coefficient of variation in papaya

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    Jeferson Pereira Ferreira

    2016-04-01

    Full Text Available ABSTRACT The objective of this work was to study the distribution of values of the coefficient of variation (CV in the experiments of papaya crop (Carica papaya L. by proposing ranges to guide researchers in their evaluation for different characters in the field. The data used in this study were obtained by bibliographical review in Brazilian journals, dissertations and thesis. This study considered the following characters: diameter of the stalk, insertion height of the first fruit, plant height, number of fruits per plant, fruit biomass, fruit length, equatorial diameter of the fruit, pulp thickness, fruit firmness, soluble solids and internal cavity diameter, from which, value ranges were obtained for the CV values for each character, based on the methodology proposed by Garcia, Costa and by the standard classification of Pimentel-Gomes. The results obtained in this study indicated that ranges of CV values were different among various characters, presenting a large variation, which justifies the necessity of using specific evaluation range for each character. In addition, the use of classification ranges obtained from methodology of Costa is recommended.

  11. Quality of life associated factors in Chileans hospitals nurses Factores asociados a calidad de vida de enfermeras hospitalarias chilenas Fatores associados a qualidade de vida de enfermeiras hospitalares chilenas

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    Lenka Andrades Barrientos

    2007-06-01

    Full Text Available This study is focused on knowing the Quality of Life of hospital nurses, and associated factors. People surveyed are composed of 100 female nurses from a hospital, eighth region, Chile. The measuring method is a questionnaire - oriented to know bio-social-demographic variables that influence nurses - and the WHOQOL-BREF quality of life measuring scale, validated in Chilean population. Results show that Domain Social Relationship (mean=77,38 is perceived as the best by female nurses, and Physical as the worst (mean=54,56. Global Quality of Life is seen as "Good" (mean=3,99 and Health Quality of Life is perceived as "Conforming" (mean=3,97. Variables predicted for Quality of Life are: age, couple situation and night shifts. Given the results, being a female nurse, and her threefold role; new studies for deeper research, especially on those variables that were not statistically relevant, are suggested.Estudio cuyo objetivo fue conocer la Calidad de Vida de enfermeras hospitalarias, y factores asociados. El universo, con el cual se trabajó, estuvo constituido por 100 enfermeras de un hospital, octava región, Chile. El instrumento constó de un cuestionario, cuya finalidad fue conocer variables biosociodemográficas influyentes, y, la escala de medición de Calidad de Vida WHOQOL-BREF, validada en población chilena. Los resultados indican que el Dominio Relaciones Sociales (media=77,38 es el mejor percibido por estas enfermeras y, el peor, el Físico (media=54,56. La Calidad de Vida Global, fue conceptuada como "Buena" (media=3,99, y, la Calidad de Vida en Salud, se percibe "Conforme" (media=3,97. Las variables que resultaron predictoras para la Calidad de Vida son: edad, situación de pareja y realizar turnos de noche. Dado los resultados, la condición de mujer de las enfermeras y su triple rol, se sugieren nuevos estudios que profundicen, especialmente, en aquellas variables que no resultaron estadísticamente significativas.O presente estudo

  12. Canto para una semilla: Luis Advis, Violeta Parra y la modernización de la música popular chilena

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    avier Osorio Fernández

    2006-06-01

    Full Text Available El objetivo del presente artículo es aportar algunas reflexiones sobre la obra Canto para una semilla del compositor chileno Luis Advis (1972, fundamentalmente a partir de sus dimensiones culturales e históricas. El significado de esta obra es observado en relación con los debates y cuestionamientos a los que se ve enfrentado el compositor en la sociedad chilena durante las décadas de 1960 y 1970, los cuales se constituyen como expresión de las transformaciones provocadas por la modernización en nuestro país. Esta experiencia concreta de la modernidad se ve reflejada en el contenido poético de las décimas de Violeta Parra, que constituyen el soporte dramático de la obra de Luis Advis, así como también en las vinculaciones entre música culta y música popular, que darán origen posteriormente a la Nueva Canción Chilena y, con ello, redefinirán también el lugar que la música popular posee en nuestra sociedadThis article is a study of the piece Canto para una semilla (Song for a Seed by the Chilean composer Luis Advis (1972, primarily in its cultural and historical dimensions. The meaning of the work is sought in its interrelations with the problems and debates within the Chilean society of the 1960s and 1970s, which were related to the transformations brought about by modernization in Chile. This is mirrored in the poetic content of the décimas written by Violeta Parra, which constitute the dramatic support for Luis Advis's work. This is further related to the connections between art music and popular music which will later originate the Nueva Canción Chilena (New Chilean Song this redefining the place of popular music in our society

  13. Fermented Papaya Preparation Restores Age-Related Reductions in Peripheral Blood Mononuclear Cell Cytolytic Activity in Tube-Fed Patients

    Science.gov (United States)

    Fujita, Yuhzo; Tsuno, Haruo; Nakayama, Jiro

    2017-01-01

    Tube-fed elderly patients are generally supplied with the same type of nutrition over long periods, resulting in an increased risk for micronutrient deficiencies. Dietary polyphenols promote immunity and have anti-inflammatory, anti-carcinogenic, and anti-oxidative properties. Carica papaya Linn. is rich in several polyphenols; however, these polyphenols are poorly absorbed from the digestive tract in their original polymerized form. Therefore, we determined the molecular components of a fermented Carica papaya Linn. preparation, as well as its effects on immunity and the composition of gut microbiota in tube-fed patients. Different doses of the fermented C. papaya L. preparation were administered to three groups of tube-fed patients for 30 days. Its effects on fecal microbiota composition and immunity were assessed by 16S rRNA gene sequencing and immune-marker analysis, respectively. The chemical composition of the fermented C. papaya L. preparation was analyzed by capillary electrophoresis- and liquid chromatography- time of flight mass spectrometry. The fermented C. papaya L. preparation restored peripheral blood mononuclear cell (PBMC) cytolytic activity; however, no other biomarkers of immunity were observed. Treatment with the preparation (9 g/day) significantly reduced the abundance of Firmicutes in the fecal microbiota. In particular, treatment reduced Clostridium scindens and Eggerthella lenta in most patients receiving 9 g/day. Chemical analysis identified low-molecular-weight phenolic acids as polyphenol metabolites; however, no polymerized, large-molecular-weight molecules were detected. Our study indicates that elderly patients who are tube-fed over the long-term have decreased PBMC cytolytic activity. In addition, low-molecular-weight polyphenol metabolites fermented from polymerized polyphenols restore PBMC cytolytic activity and modulate the composition of gut microbiota in tube-fed patients. PMID:28060858

  14. In silico cloning and characterization of the TGA (TGACG MOTIF-BINDING FACTOR) transcription factors subfamily in Carica papaya.

    Science.gov (United States)

    Idrovo Espín, Fabio Marcelo; Peraza-Echeverria, Santy; Fuentes, Gabriela; Santamaría, Jorge M

    2012-05-01

    The TGA transcription factors belong to the subfamily of bZIP group D that play a major role in disease resistance and development. Most of the TGA identified in Arabidopsis interact with the master regulator of SAR, NPR1 that controls the expression of PR genes. As a first approach to determine the possible involvement of these transcription factors in papaya defense, we characterized Arabidopsis TGA orthologs from the genome of Carica papaya cv. SunUp. Six orthologs CpTGA1 to CpTGA6, were identified. The predicted CpTGA proteins were highly similar to AtTGA sequences and probably share the same DNA binding properties and transcriptional regulation features. The protein sequences alignment evidenced the presence of conserved domains, characteristic of this group of transcription factors. The phylogeny showed that CpTGA evolved into three different subclades associated with defense and floral development. This is the first report of basal expression patterns assessed by RT-PCR, from the whole subfamily of CpTGA members in different tissues from papaya cv. Maradol mature plants. Overall, CpTGA1, CpTGA3 CpTGA6 and CpTGA4 showed a basal expression in all tissues tested; CpTGA2 expressed strongly in all tissues except in petioles while CpTGA5 expressed only in petals and to a lower extent in petioles. Although more detailed studies in anthers and other floral structures are required, we suggest that CpTGA5 might be tissue-specific, and it might be involved in papaya floral development. On the other hand, we report here for the first time, the expression of the whole family of CpTGA in response to salicylic acid (SA). The expression of CpTGA3, CpTGA4 and CpTGA6 increased in response to SA, what would suggest its involvement in the SAR response in papaya.

  15. Identification and expression of C2H2 transcription factor genes in Carica papaya under abiotic and biotic stresses.

    Science.gov (United States)

    Jiang, Ling; Pan, Lin-jie

    2012-06-01

    C2H2 proteins belong to a group of transcription factors (TFs) existing as a superfamily that plays important roles in defense responses and various other physiological processes in plants. The present study aimed to screen for and identify C2H2 proteins associated with defense responses to abiotic and biotic stresses in Carica papaya L. Data were collected for 47,483 papaya-expressed sequence tags (ESTs). The full-length cDNA nucleotide sequences of 87 C2H2 proteins were predicated by BioEdit. All 91 C2H2 proteins were aligned, and a phylogenetic tree was constructed using DNAman. The expression levels of 42 C2H2 were analyzed under conditions of salt stress by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Methyl jasmonate treatment rapidly upregulated ZF(23.4) and ZF(30,912.1) by 18.6- and 21.7-fold, respectively. ZF(1.3), ZF(138.44), ZF(94.49), ZF(29.160), and ZF(20.206) were found to be downregulated after low temperature treatment at very significant levels (p papaya ringspot virus pathogen. ZF(30,912.1) was subcellularly localized in the nucleus by a transgenic fusion of pBS-ZF(30,912.1)-GFP into the protoplast of papaya. The results of the present study showed that ZF(30,912.1) could be an important TF that mediates responses to abiotic and biotic stresses in papaya.

  16. PREVALENCIA DE PROCEDIMIENTOS INVASIVOS EN UNA POBLACIÓN CHILENA USUARIA DE MÉTODOS DE CRIBADO Y DIAGNÓSTICO PRENATAL

    OpenAIRE

    Parra C,Mauro; Quiroz V,Lorena; Pérez B,Sebastián; Rau M,Carlos; Terra A,Rodrigo; Pedraza S,Daniel; Muñoz S,Hernán; Valdés R.,Enrique

    2007-01-01

    Objetivo: Evaluar la prevalence del uso de procedimientos invasivos prenatales en una población chilena con cribado y diagnóstico de alteraciones morfológicas y/o cromosómicas. Método: Estudio descriptivo en 17.906 embarazadas entre junio de 2000 y octubre de 2006, a las cuales se les realizó cribado ecogrático de aneuploidia y malformaciones congénitas durante el primer y segundo trimestre. Las pacientes fueron informadas y aconsejadas acerca de las implicancias de dichos procedimientos. Los...

  17. AUMENTO DE LA COMPETITIVIDAD DE LA ACUICULTURA CHILENA CON UNA PRODUCCION MAS AMISTOSA CON EL MEDIO AMBIENTE, MANEJANDO LA MICROBIOTA AUTOCTONA

    OpenAIRE

    ZUNINO VENEGAS, HUGO

    2013-01-01

    Chile es uno de los principales exportadores mundiales de salmónidos. A pesar del presente éxito de la industria acuícola chilena, existen problemas no resueltos que le resta competitividad. Entre las amenazas más importantes están las infecciones por patógenos que causan muerte o enfermedades, que se traducen en retardo del crecimiento y pérdida de peso, provocando pérdidas financieras importantes a esta industria. Las infecciones ocasionan, además, problemas asociados de naturaleza am...

  18. Análisis de actividades sobre probabilidad en libros de texto para un curso de básica chilena

    OpenAIRE

    Díaz-Levicoy, Danilo; Roa, Rafael

    2014-01-01

    El presente artículo muestra los resultados de una investigación sobre las actividades propuestas en tres libros de texto, para el octavo año de educación básica chilena, en el tema de probabilidad. Estas actividades se han clasificado en ejercicios y problemas, estos últimos según la propuesta de Díaz y Poblete (1999). Se utilizó una metodología cualitativa, mediante el análisis de contenido. Dentro de los resultados de destacan: diferencia en la estructura de los libros de texto; predominio...

  19. DEL CHILE DE LOS TRIUNFOS MORALES AL PAIS GANADOR. uNA HISTORIA DE LA SELECCION CHILENA DE FUTBOL DURANTE LA DICTADURA MILITAR. 1973-1989

    OpenAIRE

    VILCHES PARRA, DIEGO

    2013-01-01

    La presente tesis es una historia de la selección chilena de fútbol durante la Dictadura Militar encabezada por el General Augusto Pinochet. En ésta se plantea que la concepción social del fútbol cambió radicalmente durante este decisivo período de la historia nacional. Específicamente, se produjo el paso desde una valoración del fútbol más cercana a un juego y, por lo tanto, en la que los triunfos morales eran apreciados; hacia una en la que se vuelven hegemónicos los valores competitivos de...

  20. La inclusión educativa desde la voz de madres de estudiantes con Trastornos del Espectro Autista en una muestra chilena

    OpenAIRE

    2014-01-01

    El objetivo del estudio es mostrar la perspectiva de madres chilenas sobre la inclusión educativa de sus hijos escolarizados en centros de educación primaria, quienes presentan un Trastorno del Espectro Autista. Se recogen evidencias, desde la voz de las familias, que pueden servir como punto de partida para reflexionar sobre cómo avanzar hacia una educación más inclusiva y cómo contribuir a mejorar los apoyos que se brindan a estudiantes con discapacidad. El estudio se enmarca en un paradigm...

  1. Regional journals in medicine and public health: a look to the future upon the indexing of the Revista Chilena de Infectología.

    Science.gov (United States)

    Vermund, Sten H; Acuña, Guillermo

    2005-03-01

    Revista Chilena de Infectología has recently been accepted for index listing in the Index Medicus via the MEDLINE data base. This breakthrough prompts reflection on the nature of regionally focused subspecialty journals and what such a listing means for better dissemination of scientific and medical discoveries reported in such journals. There is a conscientious global effort at present to better represent regional journals in indexing systems and to make access easier for persons in less wealthy nations to medical and public health information via the internet. These trends are reviewed and discussed in the context of Chile's national medical and public health literature.

  2. Cuando la memoria reconstruye la historia. El “género negro” en la literatura chilena contemporánea

    Directory of Open Access Journals (Sweden)

    Gilda Waldman M.

    2001-01-01

    Full Text Available Este artículo analiza el papel que ha jugado el “género negro” en la nueva narrativa chilena, como una forma alusiva y tangencial de desenmascarar lo sucedido durante los últimos años de la historia de ese país. El texto se detiene, en especial, en el enfoque crítico implícito en el relato de serie negra y en la reconstrucción, a partir de una temática criminológica, de los conflictos políticos y sociales del Chile dictatorial y postdictatorial.

  3. Conocimientos y autoeficacia asociados a la prevención del VIH y SIDA en mujeres chilenas

    Science.gov (United States)

    Villegas Rodríguez, Natalia; Ferrer Lagunas, Lilian Marcela; Cianelli Acosta, Rosina; Miner, Sarah; Lara Campos, Loreto; Peragallo, Nilda

    2014-01-01

    Resumen Objetivo Evaluar la relación existente entre conocimientos y autoeficacia asociados al VIH/SIDA en mujeres chilenas en desventaja social. Metodología Estudio correlacional, que utiliza la medición basal del estudio “Testeando una intervención en VIH y SIDA en mujeres chilenas”, realizada entre 2006 y 2008, que tiene una muestra de 496 mujeres entre 18 y 49 años residentes en dos comunas de Santiago de Chile. Las participantes respondieron un cuestionario estructurado aplicado por entrevistadoras entrenadas. Este cuestionario incluyó preguntas sobre datos sociodemográficos, escala de conocimientos de conductas de riesgo y autoeficacia, entre otros. Resultados Edad promedio de 32.3±9.1 años, 72.2% vive con su pareja y 42.7% poseen educación media completa. La puntuación media de los conocimientos de la infección por el VIH fue de 8.9±2.5, mientras que para las tres escalas empleadas para medir autoeficacia fueron: “Normas de los pares” =9.8±3.6, “Intención de reducir conductas de riesgo” =12.2±3.6 y “Self Efficacy Form”=20.2±4.7. Los conocimientos tuvieron una correlación positiva débil con la “intención de reducir conductas de riesgo” (r=0.19; psocial. PMID:25284914

  4. Feminización de la escuela básica chilena. Metáforas de la identidad docente

    Directory of Open Access Journals (Sweden)

    Claudia González Castro

    2015-10-01

    Full Text Available Este artículo invita a reflexionar sobre los cuestionamientos a la calidad de la educación básica en Chile, desde una perspectiva de género. Para ello, se analizó la praxis discursiva de un grupo de estudiantes de Pedagogía, a través de una entrevista grupal, identificando las metáforas que conforman su discurso y que, en su interpretación, contienen la caracterización de su propia identidad docente. Concluimos que, por encima de los atributos profesionales, la educación es una profesión altamente feminizada, sobre la cual recae el imaginario del apostolado maternal y otros principios propios del estereotipo de género. En esta feminización de la docencia puede estar entrampada la calidad de la escuela básica chilena, como institución imaginariamente femenina. AbstractThis article invites us to reflect on inquiry of the quality of basic education in Chile, from a gender perspective. To do this, we analyzed the discursive praxis of a group of students of pedagogy through a group interview, identifying the metaphors that make their speech, which shows the characterization of their own teacher identity. We conclude that, beyond the professional attributes, education in Chile is a profession exercised mostly by women, on which rests the maternal apostolate and other principles inherent in the female gender stereotype. This over-population of female teachers can be the reason for the quality of basic education in Chile to be stuck.

  5. La construcción discursiva de los imaginarios sociales: el caso de la medicina popular chilena

    Directory of Open Access Journals (Sweden)

    Gerardo Álvarez

    2002-09-01

    Full Text Available En este artículo se da cuenta del estado de avance de una investigación interdisciplinaria (lingüística, psicología, sociología sobre las representaciones de cuerpo (salud/enfermedad en la medicina popular chilena (Proyecto FONDECYT 1000376. No se trata de una investigación sobre la medicina como tal. El objetivo es rastrear el imaginario sobre el cuerpo que subyace a las prácticas curativas y que se revela a través del discurso de las sanadoras populares (mapuches y no mapuches, y luego examinar las relaciones que se establecen entre estos dos tipos de saberes: el saber médico científico "occidental" y los saberes tradicionales populares. El método de análisis deriva de la teoría de la enunciación, de la polifonía enunciativa y del análisis actancialde los relatos. (This article deals with the representation of the human body (in terms of health and sickness which underlies the curative practices of popular Chilean "curanderas" and "meicas" (Research project: FONDECYT 1000376. Through an interdisciplinary approach –linguistic, psychological and sociological– the authors examine the imagery of the human body that is revealed through discoursive practices, and the relationship between the scientific "western" medical representations and those of popular medicine women (mapuche and "hispanic". The analysis of interviews draws mainly upon the theories of enunciation (Benveniste, intertextuality (Bakhtin and the actantial analysis of tales (Greimas.

  6. 木瓜酸奶制作工艺的优化%Papaya yogurt production process optimization

    Institute of Scientific and Technical Information of China (English)

    孟君; 范秉琳; 白会丽

    2014-01-01

    [Objective]The processing conditions of papaya dairy products were optimized to provide a new way for developing new types of fruit and vegetable yogurt and fortified milk. [Method]Papaya and fresh milk were used as raw ma-terials. The finished products of yogurt were used as a leavening agent. Juice yogurt was prepared with added sugar and stabi-lizer after sterilization, cooling and fermentation. The processing conditions of papaya dairy products were optimized based on single factor test and orthogonal test. [Result]The best ratio of raw materials for papaya yogurt was as follows:milk, papaya juice:sugar:yeast leavening agent (Bright probiotics fermented milk):stabilizer (CMC∶gelatin=1∶1)=100∶30∶12∶60∶0.2. The production process of fermentation was 7 h fermentation at 40 ℃and 12 h refrigerator cooling. In the conditions of optimum process, the papaya yogurt acidity was 90.02 °T. Protein content was 3.09 g/100 g. Ca, Fe and Zn content was 121.75, 3.8575, and 5.0275 mg/L, respectively.[Conclusion]The papaya yogurt was prepared using papaya and fresh milk as raw materials and commercially available yogurt as the leavening agent. It was easy to obtain raw materials. The method was simple and feasible. The fermentation time was short, hence it was particularly suitable for home-made papaya yogurt.%【目的】优化木瓜酸奶的制作工艺条件,为研制新型的果蔬酸奶及营养强化乳提供新途径。【方法】以木瓜和鲜牛奶为原料,以成品酸奶为发酵剂,加入蔗糖、稳定剂,经杀菌、冷却后发酵制备果汁型酸奶,并通过单因素试验和正交试验优化木瓜酸奶制品的工艺条件。【结果】木瓜酸奶的最佳原料配比为鲜牛奶∶木瓜汁∶蔗糖∶发酵剂(光明益生菌发酵乳)∶稳定剂(CMC∶明胶=1∶1)=100∶30∶12∶60∶0.2,生产工艺为40℃发酵7 h后放入4℃冰箱冷藏12 h。在最佳工艺条件下制备获得的木瓜酸奶酸度为90.02

  7. La inclusión educativa desde la voz de madres de estudiantes con Trastornos del Espectro Autista en una muestra chilena

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    Marcela Villegas Otárola

    2014-12-01

    Full Text Available El objetivo del estudio es mostrar la perspectiva de madres chilenas sobre la inclusión educativa de sus hijos escolarizados en centros de educación primaria, quienes presentan un Trastorno del Espectro Autista. Se recogen evidencias, desde la voz de las familias, que pueden servir como punto de partida para reflexionar sobre cómo avanzar hacia una educación más inclusiva y cómo contribuir a mejorar los apoyos que se brindan a estudiantes con discapacidad. El estudio se enmarca en un paradigma cualitativo de investigación, utilizando entrevistas no estructuradas para acceder a la información. Se ha contado con la participación de ocho madres chilenas de la Región de Valparaíso. Los resultados muestran que la valoración de la escolarización está estrechamente relacionada con el bienestar emocional, ámbito al que se le otorga mayor relevancia que al de los resultados académicos. Se destaca la importancia de los apoyos adaptados a las necesidades del sistema familiar.

  8. PENGGUNAAN GETAH PEPAYA DALAM SINTESIS ESTER XILITOL ASAM LEMAK (EXAL [The Use of Papaya Exudates for Fatty Acid Xilitol Synthesis

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    Suhardi

    2003-12-01

    Full Text Available Fatty acid xylitol-ester (FAXILE are xylitol esters of fatty acids with one to five DE (degree of esterification value. FAXILE with DE value of 3 or higher can be used as low calorie fat replacer since they have low digestive property; while the xylitol ester with DE of less than 3 can be used as emulsifier. The FAXILE synthesis experiments were carried out by esterification of xylitol with palm oil¡¦s fatty acid (POFA using papaya exudates as a lipase source. The objective of this experiment was to evaluate the potential of papaya exuadates for the synthesis of FAXILE. In this first experiment, test were carried out to determine hydrolytic activity of lipases obtained from different part of papaya plant. The test, were performed with the presence of CaO and under different pH value. The papaya exudates were used for FAXILE synthesis under an optimum condition obtained from the first experiment. Samples were drawn during incubation at 40„aC for 1,2,3, and 4 days and hydroxyl number was analyzed to confirm the ester formation. The results showed that the most active exudate was from papaya leaves, followed by exudates from fruit with hydrolytic activity of 653 and 296 ƒÝmol/g. minutes, respectively. The hydrolytic activity of the fruit exudate was optimum at pH 6.0, at 45-50„aC, with the addition of CaO 4% dry exudates. The FAXILE synthesis with acid of papaya exudates was optimum at pH 6.0, at 45„aC with molar ratio of xylitol: fatty acids was 1 to 6,3 days incubation. At this condition the conversion rate xylitol to FAXILE was 79%.

  9. RNA interference of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO1 and ACO2) genes expression prolongs the shelf life of Eksotika (Carica papaya L.) papaya fruit.

    Science.gov (United States)

    Sekeli, Rogayah; Abdullah, Janna Ong; Namasivayam, Parameswari; Muda, Pauziah; Abu Bakar, Umi Kalsom; Yeong, Wee Chien; Pillai, Vilasini

    2014-06-19

    The purpose of this study was to evaluate the effectiveness of using RNA interference in down regulating the expression of 1-aminocyclopropane-1-carboxylic acid oxidase gene in Eksotika papaya. One-month old embryogenic calli were separately transformed with Agrobacterium strain LBA 4404 harbouring the three different RNAi pOpOff2 constructs bearing the 1-aminocyclopropane-1-carboxylic acid oxidase gene. A total of 176 putative transformed lines were produced from 15,000 calli transformed, selected, then regenerated on medium supplemented with kanamycin. Integration and expression of the targeted gene in putatively transformed lines were verified by PCR and real-time RT-PCR. Confined field evaluation of a total of 31 putative transgenic lines planted showed a knockdown expression of the targeted ACO1 and ACO2 genes in 13 lines, which required more than 8 days to achieve the full yellow colour (Index 6). Fruits harvested from lines pRNAiACO2 L2-9 and pRNAiACO1 L2 exhibited about 20 and 14 days extended post-harvest shelf life to reach Index 6, respectively. The total soluble solids contents of the fruits ranged from 11 to 14° Brix, a range similar to fruits from non-transformed, wild type seed-derived plants.

  10. RNA Interference of 1-Aminocyclopropane-1-carboxylic Acid Oxidase (ACO1 and ACO2 Genes Expression Prolongs the Shelf Life of Eksotika (Carica papaya L. Papaya Fruit

    Directory of Open Access Journals (Sweden)

    Rogayah Sekeli

    2014-06-01

    Full Text Available The purpose of this study was to evaluate the effectiveness of using RNA interference in down regulating the expression of 1-aminocyclopropane-1-carboxylic acid oxidase gene in Eksotika papaya. One-month old embryogenic calli were separately transformed with Agrobacterium strain LBA 4404 harbouring the three different RNAi pOpOff2 constructs bearing the 1-aminocyclopropane-1-carboxylic acid oxidase gene. A total of 176 putative transformed lines were produced from 15,000 calli transformed, selected, then regenerated on medium supplemented with kanamycin. Integration and expression of the targeted gene in putatively transformed lines were verified by PCR and real-time RT-PCR. Confined field evaluation of a total of 31 putative transgenic lines planted showed a knockdown expression of the targeted ACO1 and ACO2 genes in 13 lines, which required more than 8 days to achieve the full yellow colour (Index 6. Fruits harvested from lines pRNAiACO2 L2-9 and pRNAiACO1 L2 exhibited about 20 and 14 days extended post-harvest shelf life to reach Index 6, respectively. The total soluble solids contents of the fruits ranged from 11 to 14° Brix, a range similar to fruits from non-transformed, wild type seed-derived plants.

  11. Effect of irradiation on color of minimally processed melon and papaya

    Energy Technology Data Exchange (ETDEWEB)

    Fabbri, Adriana D.T.; Sagretti, Juliana M.A.; Hirashima, Fabiana K.; Rogovschi, Vladimir D.; Nunes, Thaise C.F.; Sabato, Susy F., E-mail: adriana.fabbri@yahoo.com.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil). Lab. de Irradiacao de Alimentos

    2013-07-01

    The access to nutritious food is an essential dimension of food meal. High potential for fresh-cut industry exists and ready-to-eat fruit market has grown rapidly in recent years due to the health benefits associated. Although there is many concerns to food safety other parameters like texture, taste, color and sensory acceptance are fundamental principles of acceptance to any food. Actually the use of instrumental measurements has proven to be a major predictor of sensory responses. According to many authors, the addition of different techniques should always be considered to provide additional information of the sensory aspects. Therefore the objective of this study was to evaluate the influence of irradiation on color of minimally processed melon and papaya. The fruits were purchased in a market of Sao Paulo, at the same point of ripeness and sent to the IPEN/CNEN-SP. The fruits were sanitized and manually cut into cubes of approximately 2 x 2 cm with the aid of stainless steel knives and packed in polyethylene bags. Melons and papaya were irradiated in a Multipurpose Gamma Source (IPEN - Sao Paulo - Brazil) and were divided in six groups for color analysis: Control; 0.5 kGy; 1.0 kGy, 1.5kGy, 2.0 kGy and 3.0kGy. After the treatment, the MP fruits were kept in a refrigerator at 4 deg C ± 1 deg C until the end of the analysis. The color of the samples was determined using a Minolta colorimeter CR-400 Chromameter. The parameters L{sup *}, a{sup *} and b{sup *} were evaluated. The results were statistically analyzed by analysis of variance One-Dimensional Analysis of Variance (One-Way-ANOVA) followed by Tukey test. All statistical analysis was performed using the program Graph Pad Prism 5 and adopting a significance level of 5 % (p<0.05), expressed as the mean results ± standard deviation. Samples of papaya and melons showed no statistical difference for the L{sup *} parameter of any dose despite the tendency to darkening observed for the group of 3.0 kGy. This

  12. Enantioselective esterification of (R,S)-2-methylalkanoic acid with Carica papaya lipase in organic solvents.

    Science.gov (United States)

    Chang, Chun-Sheng; Ho, Ssu-Ching

    2011-11-01

    Isooctane was the best reaction medium for the enantioselective esterification of (R,S)-2-methylalkanoic acid with n-butanol using Carica papaya lipase as catalyst. Increasing linear alkyl-chain length of racemic 2-methylalkanoic acids from ethyl to hexyl increased the enantioselectivity (E) from 2.1 to 98.2 for the esterification of racemic 2-methylalkanoic acids with n-butanol at 35°C. Decreasing reaction temperature from 40 to 20°C increased the enantioselectivity (E) from 14 to 33 for the esterification of racemic 2-methylhexanoic acids with n-butanol. We obtained a maximum enantioselectivity, of E = 24.3, for the enantioselective esterification of racemic 2-methylhexanoic acids with n-butanol in isooctane at water activity 0.33, and at 35°C.

  13. Process variables influence on microwave assisted extraction of pectin from waste Carcia papaya L. peel.

    Science.gov (United States)

    Maran, J Prakash; Prakash, K Arun

    2015-02-01

    The objectives of the present study were to evaluate and optimize the influence of process variables such as microwave power, pH, time and solid-liquid ratio on the extraction of pectin from waste Carcia papaya L peel. The experiments were carried out based on a four factors three level Box-Behnken response surface design. A quadratic model was developed from the experimental data in order to predict the pectin yield. The optimal condition was found to be: microwave power of 512 w, pH of 1.8, time of 140 s and solid-liquid ratio of 1:15 g/ml with maximum pectin yield (25.41%).

  14. The minimal 5' sequence for in vitro initiation of papaya mosaic potexvirus assembly.

    Science.gov (United States)

    Sit, T L; Leclerc, D; AbouHaidar, M G

    1994-02-15

    The minimal 5' initiation of assembly sequence for papaya mosaic virus (PMV) has been determined by the generation of successive 3' deletion clones from a full-length cDNA copy of the PMV genome followed by in vitro assembly of RNA transcripts. Sequences closest to the 5' terminus were cloned into plasmid vectors with synthetic complementary oligonucleotides representing viral sequences. The in vitro initiation sequence has been narrowed down to 38-47 nucleotides at the 5' terminus which are capable of initiation alone or in conjunction with homologous or heterologous sequences. The minimum initiation sequence is rich in adenosine and cytidine and very poor in uridine nucleotides and lacks any discernible secondary structure.

  15. Antioxidant activities and phenolics profiling of different parts of Carica papaya by LCMS-MS.

    Science.gov (United States)

    Zunjar, V; Mammen, D; Trivedi, B M

    2015-01-01

    This article deals with the comparison of the antioxidant activity of aqueous extracts of various parts of Carica papaya L. The evaluation of total phenolic content and total flavonoid content revealed high antioxidant potential of the seeds and fruits. The free radical-scavenging potential of the aqueous extracts indicated the seeds to have better DPPH-scavenging activity than fruits. The results were augmented by the FRAP activity as well. The phenolics present in the extracts were separated and identified as 5-hydroxy feruloyl quinic acid, acetyl p-coumaryl quinic acid, quercetin-3-O-rhamnoside, syringic acid hexoside, 5-hydroxy caffeic quinic acid, peonidin-3-O-glucoside, sinapic acid-O-hexoside, cyaniding-3-O-glucose and methyl feruloyl glycoside by LCMS-MS technique.

  16. Radiation preservation of foods of plant origin. III. Tropical fruits: bananas, mangoes, and papayas

    Energy Technology Data Exchange (ETDEWEB)

    Thomas, P.

    1986-01-01

    The current status of research on the use of ionizing radiation for shelf life improvement and disinfestation of fresh tropical fruits like bananas, mangoes, and papayas are reviewed. The aspects covered are influence of maturity and physiological state of the fruits on delayed ripening and tolerance to radiation; varietal responses; changes in chemical constituents, volatiles, respiration, and ethylene evolution; biochemical mechanisms of delayed ripening and browning of irradiated fruits; and organoleptic quality. The efficacy of the combination of hot water dip and radiation treatments for control of postharvest fungal diseases are considered. The immediate potential of radiation as a quarantine treatment, in place of the currently used chemical fumigants, for disinfestation of fruit flies and mango seed weevil are discussed. Future prospects for irradiation of tropical fruits are discussed in the light of experience gained from studies conducted in different countries.146 references.

  17. GROWTH OF MACROBRACHIUM ROSENBERGII FED WITH MANGO SEED KERNEL, BANANA PEEL AND PAPAYA PEEL INCORPORATED FEEDS

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    P. Aarumugam

    2013-06-01

    Full Text Available The growth promoting potential of fruits wastes, mango seed kernel, banana peel and papaya peel on the freshwater prawn, Macrobrachium rosenbergii post larvae (PL was evaluated. Basal diet equated to 35% protein was prepared by using soybean meal, groundnut oilcake, horse gram and wheat flour. Each fruit waste powder was separately incorporated with basal diet at a proportion of 10%. Sunflower oil was used as lipid source. Egg albumin and tapioca flour were used as binding agents. Vitamin B-complex with Vitamin-C was also mixed. Feed without any fruit waste was served as control. M. rosenbergii PL (length: 1.2-1.4 cm; weight: 0.09- 0.13 g was fed with these feeds for a period of 90 days. Significant improvements in the nutritional indices (survival rate, weight gain, biomass index, specific growth rate and condition factor, concentrations of biochemical constituents (total protein, carbohydrate and lipid, levels of non-enzymatic antioxidants (vitamin-C and E, content of minerals (Na+ and K+, activities of digestive enzymes (protease, amylase and lipase, and profiles of essential amino acids and fatty acids were recorded in fruits wastes incorporated feeds fed PL when compared with control (P< 0.003 – 0.878. The overall results indicated the fact that mango seed kernel incorporated feed was produced the best performance, followed by better performance of banana peel and good performance of papaya peel. These fruits wastes incorporated feeds enhance digestive enzymes activities and act as appetizer, which in turn enhances food utilization and ultimately yielded better survival and growth of M. rosenbergii PL. Therefore, these fruits wastes have considerable potentials in sustainable development of Macrobrachium culture.

  18. Dormancia en semillas de papaya cv Maradol Roja durante el almacenamiento

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    Maruchi Alonso-Esquivel

    2011-01-01

    Full Text Available El objetivo del presente trabajo fue determinar el efecto de la humedad de la semilla cultivar Maradol Roja y la temperatura durante el almacenamiento, en la dormancia de la papaya. Este trabajo fue desarrollado en el Laboratorio Nacional de Semillas de la Empresa Productora y Comercializadora de Semillas, provincia La Habana, Cuba, en el período noviembre 2006 a enero 2008. Fueron extraídas las semillas de frutos de papaya cv Maradol Roja, y se secaron a tres contenidos de humedad (12%, 10,56% y 9,26%, y acondicionadas en bolsas de polietileno y almacenadas a 15ºC y 4ºC. El porcentaje de germinación, altura y peso fresco de la planta fueron realizadas al inicio y después de tres, seis, nueve y doce meses de almacenamiento. Hubo tendencia a la disminución de la germinación en los primeros seis mess del almacenamiento, alcanzándose los valores más bajos del porcentaje de germinación (55,6%. A partir de los nueve y doce meses fue detectado un aumento signifi cativo en la germinación, independiente del contenido de humedad de la semilla. El ambiente de 15ºC favoreció mayor germinación en períodos mayores de almacenamiento en relació a las mantenidas a 4ºC, que exhibieron mejores porcentajes de germinación en un período menor (tres meses de almacenamiento. La dormancia fi nalizó en los periodos de almacenamiento a los nueve y doce meses, independientemente del contenido de humedad.

  19. Lactogenic Activity of an Enzymatic Hydrolysate from Octopus vulgaris and Carica papaya in SD Rats.

    Science.gov (United States)

    Cai, Bingna; Chen, Hua; Sun, Han; Sun, Huili; Wan, Peng; Chen, Deke; Pan, Jianyu

    2015-11-01

    The traditional Chinese medicine theory believes that octopus papaya soup can stimulate milk production in lactating women. The objective of this study was to determine whether dietary supplementation with an enzymatic hydrolysate of Octopus vulgaris and Carica papaya (EHOC) could increase milk production and nutritional indexes in Sprague Dawley (SD) rats. Female SD rats (n = 24) were fed a control diet (n = 8), EHOC-supplemented diet, or a positive control diet (Shengruzhi) from day 10 of pregnancy to day 10 of lactation. Maternal serum, mammary gland (day 10 of lactation), milk, and pup weight (daily) were collected for analysis. Results showed that the EHOC diet obviously elevated daily milk yield and pup weight compared to the control group (P < .05). The EHOC diet was found to increase the concentration of prolactin (PRL), progesterone (P), estradiol (E2), and growth hormone (GH) significantly in the circulation and mammary gland. Mammary glands of EHOC-treated dams showed clear lobuloalveolar development and proliferation of myoepithelial cells, but no striking variations were observed among the groups. Furthermore, the nutrition content and immune globulin concentration in the milk of EHOC-supplemented dams were higher than those of the control group, especially the cholesterol, glucose, and IgG were higher by 44.98% (P < .001), 42.76% (P < .01), and 42.23% (P < .01), respectively. In conclusion, this article demonstrates that EHOC administration has beneficial effects on milk production in the dams and on performance of the dam and pup. These results indicate that EHOC could be explored as a potentially lactogenic nutriment for lactating women.

  20. Herencia de la concentración de los sólidos soluble entre líneas parentales de papaya (carica papaya l. y sus híbridos

    Directory of Open Access Journals (Sweden)

    Eric Mora

    2004-01-01

    Full Text Available Herencia de la concentración de los sólidos solubles entre líneas parentales de papaya (Carica papaya L. y sus híbridos. Se estableció un experimento con el objetivo de determinar el patrón de herencia de la concentración de sólidos solubles de frutas (medido como grados brix entre tres líneas de papaya y sus tres posibles híbridos. Se utilizaron como materiales parentales tres líneas genéticas con niveles de azúcares significativamente diferentes entre ellos. Se determinó que la característica de alto contenido de sólidos solubles se comportó de manera dominante sobre un bajo contenido de los mismos. Se concluyó que existe un gran potencial para explotar comercialmente este patrón dominante al posibilitar el uso de germoplasma de buenas características agronómicas pero deficientes en sus contenidos de azúcares en sus frutas. La posible naturaleza de los factores que intervienen en la característica estudiada se discuten.

  1. QUALITATIVE PHYTOCHEMICAL SCREENING AND ANTIFUNGAL ACTIVITY OF CARICA PAPAYA LEAF EXTRACT AGAINST HUMAN AND PLANT PATHOGENIC FUNGI

    Directory of Open Access Journals (Sweden)

    Sikandar Khan Sherwani

    2013-07-01

    Full Text Available Plants have been explored extensively all over the globe in quest of a novel bioactive compound that could a good therapeutic candidate treating infectious diseases especially against drug resistant microbes. Qualitative phytochemical analyses of Carica papaya leaf extract reveal that except steroids and tannins all the possible phytochemical constituents including carbohydrates, proteins, anthraquinones, flavonoids, saponins, cardiac glycosides and alkaloids were present. Two ways of Carica papaya leaf extract preparations i.e crushed and boiled were tested for their antifungal activity against 6 saprophytic fungi Penicillium sp, Aspergilus flavus, Aspergillus niger, Fusarium sp, Rhizopus and Helminthosporum, 5 dermatophytic fungi Microsporum canis, Microsporum gypseum, Trichophyton rubrum, Trichophyton mentagrophytes, Trichophyton tonsurans and 6 yeasts including Candida albicans, Candida albicans ATCC 0383, Saccharomyces cerevisiae, Candida galbrata, Candida tropicalis, Candida kruzei. The activity was found against majority of fungi but was much better in case of crushed leaf extract.

  2. Nucleotide sequence and intergeminiviral homologies of the DNA-A of papaya leaf curl geminivirus from India.

    Science.gov (United States)

    Saxena, S; Hallan, V; Singh, B P; Sane, P V

    1998-06-01

    Coat protein gene, rep protein gene and intergenic region of the genome of a whitefly transmitted geminivirus (WTG) causing severe leaf curl in papaya plants were PCR amplified, cloned and sequenced. Comparison of the amino acid sequence of the putative coat protein product of papaya leaf curl virus (PLCV) with some other mono and bipartite WTGs revealed a maximum of 89.8% homology with Indian cassava mosaic virus. The genomic organization of PLCV-India is similar to other WTGs with bipartite genomes. Comparison of the coat protein N-terminal 70 amino acid sequence (and other biological features) of PLCV with other geminiviruses shows that PLCV is a distinct geminivirus from India and is related to WTGs from the old world.

  3. 番木瓜果酒的酿制工艺%The Brewing Technology of Carica Papaya Fruit Wine

    Institute of Scientific and Technical Information of China (English)

    夏杏洲; 彭球生; 庞李生; 彭克东

    2001-01-01

    介绍了以成熟番木瓜为原料,经打浆、成分调整及采用高活性干酵母进行发酵,酿造出风味独特、品质上乘的番木瓜果酒。确定了最适工艺条件,制定了产品的质量标准。%The brewing technology of carica papaya fruit wine with special flavour and high quality by using ripe carica papaya fruit as raw material, through mashing ingredient adjusting and fermenting by using high dry yeasts is introduced in this paper, the optimized processing technology and the quality standards are determined.

  4. Effect of irradiation on the biochemical and organoleptic changes during the ripening of papaya and mango fruits

    Science.gov (United States)

    Lacroix, Monique; Bernard, Linda; Jobin, Michele; Milot, Sylvain; Gagnon, Marcel

    Papaya and mango rot caused by fungi is a major problem during storage and marketing. Gamma irradiation treatment was used to determine its effect on the quality of papayas and mangoes irradiated at 0,5 to 0,95 kGy. The level of respiration, soluble solids, texture, vitamin C and the sensorial evaluation were effectuated. The results indicate that irradiation treatment reduces significantly (p ⪕ 0,001) the level of respiration and significantly (p ⪕ 0,001) weakens the texture of mangoes. The content of soluble solids and vitamin C are not significantly affected by the irradiation. The sensory evaluation indicates that up to 0,95 kGy the sensorial quality is not changed.

  5. La escuela y la cuestión del mérito: reflexiones desde la experiencia chilena

    Directory of Open Access Journals (Sweden)

    Kathya Araujo

    2015-12-01

    Full Text Available Resumen Este artículo discute la importancia y centralidad que ha adquirido el mérito en la sociedad chilena hoy, enfocándose principalmente en sus efectos asociados con la experiencia educacional. El argumento es desarrollado en tres pasos. Luego de una presentación del contexto histórico y de las transformaciones sociales que dan cuenta de su expansión, se analizan sobre todo las maneras en que el reconocimiento del mérito es distintamente evaluado en las diferentes etapas de la trayectoria escolar por las clases medias y los sectores populares. En un tercer momento, se muestra de qué manera a través del extendido recurso a las redes sociales y a los contactos interpersonales, la expansión del mérito se acompaña del surgimiento de un nuevo horizonte de injusticia social. El artículo pone en evidencia, así, a la vez el fuerte consenso en torno al valor del mérito en Chile y los sentimientos de injusticia y frustración que ello engendra en los individuos. La discusión presentada se basa en los resultados de dos estudios cualitativos. La primera investigación se desarrolló en las ciudades de Santiago, Concepción y Valparaíso, entre 2007 y 2009, a través de alrededor de un centenar de entrevistas a hombres y mujeres de entre 30 y 55 años, pertenecientes la mitad de ellos a sectores populares urbanos y la otra mitad a las clases medias y medias-altas. La segunda investigación, en curso, aportó al análisis dieciséis entrevistas hechas en Santiago, a lo largo del 2014, a partir de las cuales se profundizó la problemática del mérito.

  6. DE ARMARIOS Y BIBLIOTECAS: MASCULINIDAD Y TRADICIÓN LITERARIA CHILENA EN LA NARRATIVA DE ALBERTO FUGUET

    Directory of Open Access Journals (Sweden)

    Cristian Opazo

    2009-04-01

    Full Text Available La tesis de este ensayo postula que la narrativa de Alberto Fuguet puede ser leída como un proyecto escriturario que propone una (nueva estrategia retórica por la cual representar los desvíos del género sexual. Según este modus operandi, en las narraciones de Fuguet, tanto el acto de reprimir como el de explicitar los desvíos de la masculinidad, se ejecutan a través de un juego de citas a la tradición literaria chilena. En los textos de José Donoso, Jorge Edwards y Alfredo Gómez Morel, entre otros, los narradores personajes de Fuguet encuentran un conjunto de figuras retóricas que les permite decir su desvío, pero de manera oblicua; es decir, cifrando la voluptuosidad de sus deseos en la lengua culturalmente prestigiosa del Canon de las Letras de Chile. Dentro de este contexto, este ensayo pesquisa las citas que los primeros trabajos de Fuguet (Sobredosis y Mala onda hacen de Casa de campo y El jardín de al lado, de José Donoso, y El río, de Alfredo Gómez Morel.This essay proposes that Alberto Fuguet s narrative may be read as a writing project with a (new rhetorical strategy by which various sexual deviances may be represented By this modus operandi, in Fuguet's stories, both manifestation and repression of male deviance are made explicit by way of a 'quotation game' in the field of Chilean literary tradition. The narrators in his texts find in the narrative of José Donoso, Jorge Edwards and Alfredo Gómez Morel, among others, a corpus of rhetorical figures which allow them to make their deviance explicit in an oblique way; that is, to condense their voluptous desire in the prestigious language of the Chilean Literary Canon. Within this context, this essay explores the quotations of Donoso 's Casa de Campo and El Jardin de al lado, and those quotations of Alfredo Garcia Morel's El río present in Alberto Fuguet's first works (Sobredosis y Mala Onda.

  7. PROYECTOS DE ELITE CHILENA DEL SIGLO XIX (I Chile’s 19th century elite’s projects

    Directory of Open Access Journals (Sweden)

    Jorge Pinto Rodríguez

    2008-07-01

    Full Text Available Examen de los proyectos con que la elite chilena del siglo XIX gobernó el país. La hipótesis es que hubo un proyecto original levantado por quienes se instalan en el poder después de la Independenciaque se sostuvo en el orden, la centralización del poder, la voluntad de desarrollarnos por nosotros mismos, de espaldas a cualquier proyecto sudamericano, y materialmente sostenido en las exportaciones de nuestras materias primas. Este proyecto fue cuestionado por una generación más liberal, formada en los años 40, que llega al poder en los años 60. Admiradores del positivismo, aquellos jóvenes vieron en Europa el modelo a seguir. Para ellos, el progreso, meta final que querían lograr, fue asociado a Francia, Inglaterra y, en algunos casos, a Estados Unidos, cuyas huellas pretenden seguir, sin considerar las particularidades de Chile. Con cierta insensibilidad social e incapaz de atender las demandas de los grupos subalternos, este proyecto arrastró al país a la crisis que lo sacudió a fines del siglo XIX y comienzos del XX.This article discusses projects with which the elite ruled in the 19th century. According to the author, there was an original project led by those who ruled the country after the ; it was based on order, power centralization, and Chilean people’s own ideas on self-improvement irrespective of any other South American project and materially supported by Chilean raw materials exports. This Project was questioned by a more liberal generation rising in the 1840s and taking over power in the 1860s. Being admirers of positivism, youngsters looked at as a model to be followed. As their ultimate goal, they associated progress with and in some cases, the They wanted to follow these countries’ footsteps regardless of ’s national characteristics. With little social sensitivity and unable to meet the demands of subaltern groups, this project led the country to the crisis at the turn of the century.

  8. White varieties of papaya production integration technology%中白品种番木瓜生产集成技术

    Institute of Scientific and Technical Information of China (English)

    陈永森

    2015-01-01

    番木瓜兼具蔬菜和水果两重属性,港澳、广东地区的居民常常用木瓜炖木瓜盅,具有去热、滋补胃脾效果,北方人喜好用木瓜片涮火锅,催熟的木瓜可以作为水果食用,具有养颜滋补、通便的效果.海南岛的生产木瓜鲜果在中秋节期间,第2年的1-4月份,北运的售价较高,近几年番木瓜全部是转基因技术的脱毒苗,花叶发病率低,许多种植户大批量生产,由于投入回报率好,岛内的许多地方发展形成大面积的产业化.%papaya vegetable and fruit twofold properties, the residents of the Hong Kong and Macao, guangdong region often use papaya pa-paya stew cup, have to heat, nourishing the stomach and spleen effect, northerners preferences with papaya rinse chaffy dish, ripened papaya can be used as a fruit to eat, have beauty nurture, aperient effect. Hainan papaya fruit during the Mid-Autumn festival, 1 to 2 years in April, the north's price is higher, virus-free papaya are all genetically modified (gm) technology in recent years, low incidence of Mosaic, many farmers in mass production, because of the good return on investment, many parts of the island's development to form a large area of industrialization.

  9. Staphylococcal enterotoxin A gene-carrying Staphylococcus aureus isolated from foods and its control by crude alkaloid from papaya leaves.

    Science.gov (United States)

    Handayani, Lita; Faridah, Didah Nur; Kusumaningrum, Harsi D

    2014-11-01

    Staphylococcus aureus is a known pathogen causing intoxication by producing enterotoxins in food. Staphylococcal enterotoxin A is one of the enterotoxins commonly implicated in staphylococcal food poisoning. The ability of crude alkaloid extract from papaya leaves to inhibit the growth of S. aureus and staphylococcal enterotoxin A synthesis was investigated. Staphylococcal enterotoxin A gene-carrying S. aureus was isolated from raw milk and ready-to-eat foods. Crude alkaloid was extracted from ground, dried papaya leaves using ultrasonic-assisted extraction, and a MIC of the alkaloid was determined by the broth macrodilution method. Furthermore, S. aureus isolate was exposed to the crude alkaloid extract at one- and twofold MIC, and the expression of sea was subsequently analyzed using a quantitative reverse transcription real-time PCR. Ten isolates of S. aureus were obtained, and nine of those isolates were sea carriers. The yield of crude alkaloid extract was 0.48 to 1.82% per dry weight of papaya leaves. A MIC of crude alkaloid to S. aureus was 0.25 mg/ml. After exposure to the alkaloid at 0.25 and 0.5 mg/ml for 2 h, a significant increase in cycle threshold values of sea was observed. The sea was expressed 29 and 41 times less when S. aureus was exposed to crude alkaloid at one- and twofold MIC, respectively. This study revealed that crude alkaloid of papaya leaves could control staphylococcal enterotoxin A gene-carrying S. aureus by suppressing the expression of sea, in addition to the ability to inhibit the growth of S. aureus. The expression of sea was successfully quantified.

  10. The amino acid sequences of eleven tryptic peptides of papaya mosaic virus protein by electron ionization mass spectrometry.

    Science.gov (United States)

    Parente, A; Short, M N; Self, R; Parsley, K R

    1982-04-01

    Eleven of the fourteen tryptic peptides of papaya mosaic virus protein have been sequenced by electron ionization mass spectrometry using chemical and enzymic hydrolyses and mixture analysis as required. Mid-chain cleavages of N-C bonds produced secondary ion series which allowed up to 16 residues to be sequenced without further hydrolysis. Mixture analysis on hydrolysis products enabled a 24 residue tryptic peptide to be sequenced from the data recorded in a single mass spectrum.

  11. The effect of praziquantel and Carica papaya seeds on Hymenolepis nana infection in mice using scanning electron microscope.

    Science.gov (United States)

    Abou Shady, Omayma M; Basyoni, Maha M A; Mahdy, Olfat A; Bocktor, Nardden Zakka

    2014-08-01

    Hymenolepis nana (H. nana) is the most common tapeworm infection worldwide. It is more prevalent in warm climates where sanitation is poor, particularly among children. The effect and mechanism of action of praziquantel (PZQ), given at a dose of 25-mg/kg BW, and Carica papaya dried seed crude aqueous extract (CAE), given at a dose of 1.2-g/kg BW, were assessed on H. nana worms in experimentally infected mice. Tegumental changes were studied using the scanning electron microscope (SEM) and different parasitological parameters were observed. Each group of infected mice was divided into two subgroups. The first subgroup received either treatment before the 4th day after infection to investigate their effects on the cysticercoid stage. The other subgroup received treatments after the development of the adult stage, confirmed by eggs detection in stool. Both PZQ and C. papaya dried seed CAE resulted in a significant reduction of worm burden, total egg output and viable egg count. Marked tegumental changes were evident in adult worms treated with either treatment including shrinkage of the scolex and neck region with rostellar edema and complete loss of its hooks. However, all previous effects were exerted more rapidly in the case of PZQ treatment. They both significantly reduced cysticercoid stage size. Nevertheless, C. papaya outstand PZQ in having a deforming effect on adults arising from treated cysticercoids. It was concluded that C. papaya has significant anti-cestodal properties that enable its seed extract to be a very effective alternative to PZQ against H. nana.

  12. Processing technology of new type papaya milk tablets%新型番木瓜奶片的研制

    Institute of Scientific and Technical Information of China (English)

    王菲; 黄苇; 郑妙慈

    2011-01-01

    The processing technique of compound papaya-milk tablets was studied.By investigating physical characteristics of the compound papaya-milk tablets via the single factor test and the L9(33)orthogonal experiment,the optimal seasoning recipe conditions were determined as follows:raw material ratio of milk powder and carica papaya powder 3∶ 1,the particle size of 20 ~ 40 mesh,maltose 30%,xylitol 20%,citric acid 0.3%,magnesium stearate dosage of 0.5%,raw material ratio of carica papaya essential oils and ethanol and water 1∶ 1∶ 1.The best quality milk tablets can be made with drying time of 6h and drying temperature of 70℃.%以番木瓜、奶粉为原料,研究了其复合奶片的制作工艺。以奶片的感官评定为指标进行单因素对比实验以及L9(33)正交优化实验,得出复合奶片最佳调味配方为:奶粉与番木瓜粉的配比为3∶1,颗粒的粒度20~40目,麦芽糖、木糖醇、柠檬酸、硬脂酸镁的添加量分别为30%、20%、0.3%、0.5%,番木瓜香精∶乙醇∶水为1∶1∶1;湿粒干燥时以70℃干燥6h,得到的奶片质量最佳,具有番木瓜香味和奶香味,口感细腻柔滑。

  13. Identification and expression of the WRKY transcription factors of Carica papaya in response to abiotic and biotic stresses.

    Science.gov (United States)

    Pan, Lin-Jie; Jiang, Ling

    2014-03-01

    The WRKY transcription factor (TF) plays a very important role in the response of plants to various abiotic and biotic stresses. A local papaya database was built according to the GenBank expressed sequence tag database using the BioEdit software. Fifty-two coding sequences of Carica papaya WRKY TFs were predicted using the tBLASTn tool. The phylogenetic tree of the WRKY proteins was classified. The expression profiles of 13 selected C. papaya WRKY TF genes under stress induction were constructed by quantitative real-time polymerase chain reaction. The expression levels of these WRKY genes in response to 3 abiotic and 2 biotic stresses were evaluated. TF807.3 and TF72.14 are upregulated by low temperature; TF807.3, TF43.76, TF12.199 and TF12.62 are involved in the response to drought stress; TF9.35, TF18.51, TF72.14 and TF12.199 is involved in response to wound; TF12.199, TF807.3, TF21.156 and TF18.51 was induced by PRSV pathogen; TF72.14 and TF43.76 are upregulated by SA. The regulated expression levels of above eight genes normalized against housekeeping gene actin were significant at probability of 0.01 levels. These WRKY TFs could be related to corresponding stress resistance and selected as the candidate genes, especially, the two genes TF807.3 and TF12.199, which were regulated notably by four stresses respectively. This study may provide useful information and candidate genes for the development of transgenic stress tolerant papaya varieties.

  14. 木瓜牛奶多肽饮料的研制%Development of papaya-milk polypeptides beverage

    Institute of Scientific and Technical Information of China (English)

    彭萍; 胡月英; 陈文学

    2012-01-01

    In this paper. A new kind of polypeptides beverage was produced with milk and papaya as main materials. We studied the effect of papaya-to-milk ratio, hydrolysis temperature, time and pH to the concentration of polypeptides by hydrolysis test and the effect of formula to the beverage quality by orthogonal experiment. The results of hydrolysis test showed that the optimum hydrolysis process was carried out at 65 ℃ and pH 6.0 for 7 h when the mass ratio of papaya to milk was 50%. The results obtained from the orthogonal experiment showed that the optimum formula was: fruit paste papaya 13.33%, milk powder 4.44%, citric acid 0.20%, β-cyclodextrin 1.25% and sucrose 15%.%以牛奶和木瓜为原料,利用木瓜自身所含的蛋白酶水解牛奶中的蛋白质.研究木瓜牛奶比例、温度、pH值、时间等因素对水解后溶液中多肽质量浓度的影响,以及配方对其风味的影响.通过水解工艺实验得到最佳水解条件为木瓜与牛奶的比例为50%,水解温度为65 ℃,水解pH值为6,水解时间为7h.通过调配实验得到木瓜牛奶多肽饮料的最佳配方为木瓜、奶粉、β-环糊精、柠檬酸、蔗糖添加量分别为13.33%,4.44%,1.25%,0.20%,15%.

  15. Study on the processing technology of Papaya Yogurt%木瓜酸奶生产工艺研究

    Institute of Scientific and Technical Information of China (English)

    刘文朵

    2015-01-01

    为优化木瓜酸奶的加工工艺,在单因素试验基础上,采用正交试验优化,其最佳生产工艺为:木瓜浆用量为10%,白砂糖用量为6%,发酵时间为4 h,接种量为4%.制得的木瓜酸奶组织均匀,口感细腻,酸甜适度.%This study mainly discussed the fermentation technology of the fermented milk with Papaya Yogurt. From the single factor experiments, the factors showed significant effects were selected in the furtherorthogonal experiments designed by Design-expert software. The optimum fermentation technology parameters were as following: the addition of papaya 10%, the addition of sugar 6%, the fermentation time 4h, inoculum, 4%. Papaya yogurt prepared under these conditions was homogeneous, with exquisite taste and moderate sour.

  16. Evaluation of the Host Status of Mature Green Papayas 'Maradol' for the Mexican Fruit Fly (Diptera: Tephritidae).

    Science.gov (United States)

    Arredondo, José; Ruiz, Lia; Díaz-Fleischer, Francisco

    2014-10-01

    The suitability of mature green 'Maradol' papaya as a host of Anastrepha ludens (Loew) was studied under field and laboratory conditions. Field tests were conducted on commercial-ripened and spot-ripened fruit in two orchards and during two seasons in the state of Chiapas. Fruits at exportation ripeness are in "commercial ripeness", while fruits that are harvested immediately preceding exportation ripeness are in "spot ripeness." The field tests consisted of forced infestation experiments that evaluated papayas at two ripeness stages: the commercial- or exportation-ripened fruit (green fruits with one or two yellow stripes) and fruit before exportation ripeness called "spot ripeness." These tests were conducted in two orchards and during two seasons in the state of Chiapas, Mexico. Laboratory trials were performed with commercial-ripened fruit only. Fruit from four different postharvest periods (3, 24, 48, and 72 h) were exposed to groups of gravid flies. No larvae emerged from the fruit that was collected in the field experiments. However, some larvae and several fertile flies were obtained from the commercial-ripened fruit 72 h postharvest but not 3, 24, and 48 h postharvest in the laboratory. The results of this study indicate that the commercially ripe fruits of papaya Maradol were resistant to or free from infestation of A. ludens flies under field conditions, though these fruits must be considered nonnatural, conditional host because they became infested in the laboratory.

  17. Effect of Fitomas-E on seedling production of papaya var. Maradol Roja in Cienfuegos,Cuba

    Directory of Open Access Journals (Sweden)

    Fernando G. Serbelló Guzmán

    2016-02-01

    Full Text Available An experiment in nursery in bag conditions was carried out at the Cienfuegos Horticultural Company, in order to evaluate the effect of different alternatives to stimulate the growth and the development of papaya seedlings (Carica papaya L. variety Maradol Red, in the period from June to September 2010. The treatments were: applying Fitomas-E, Trichoderma, EcoMic, the combination of Trichoderma + EcoMic and a control without application. We evaluated the height and diameter at four different positions, the number of leaves, green and dry mass of roots as well as for the external part of the plant at the transplantation time. The obtained data were subjected to the given statistical analyzes. Results indicate that the height and diameter of the papaya plants are increased with the application of Fitomas E and EcoMic, while a higher number of leaves is reached with the application of Fitomas E, and the combination of EcoMic with Trichoderma.

  18. Crystallization and preliminary X-ray analysis of a protease inhibitor from the latex of Carica papaya

    Energy Technology Data Exchange (ETDEWEB)

    Azarkan, Mohamed [Université Libre de Bruxelles, Faculty of Medicine, Protein Chemistry Unit, Campus Erasme (CP 609), 808 Route de Lennik, B-1070 Brussels (Belgium); Garcia-Pino, Abel [Department of Molecular and Cellular Interactions, Vlaams Interuniversitair Instituut voor Biotechnologie and Laboratorium voor Ultrastructuur, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel (Belgium); Dibiani, Rachid [Université Libre de Bruxelles, Faculty of Medicine, Protein Chemistry Unit, Campus Erasme (CP 609), 808 Route de Lennik, B-1070 Brussels (Belgium); Wyns, Lode; Loris, Remy, E-mail: reloris@vub.ac.be [Department of Molecular and Cellular Interactions, Vlaams Interuniversitair Instituut voor Biotechnologie and Laboratorium voor Ultrastructuur, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel (Belgium); Baeyens-Volant, Danielle [Université Libre de Bruxelles, Faculty of Medicine, Protein Chemistry Unit, Campus Erasme (CP 609), 808 Route de Lennik, B-1070 Brussels (Belgium)

    2006-12-01

    The Kunitz-type trypsin/chymotrypsin inhibitor isolated from C. papaya latex has been crystallized using the hanging-drop vapour-diffusion method. Two different crystal forms are observed, diffracting to 2.6 and 1.7 Å. A Kunitz-type protease inhibitor purified from the latex of green papaya (Carica papaya) fruits was crystallized in the presence and absence of divalent metal ions. Crystal form I, which is devoid of divalent cations, diffracts to a resolution of 2.6 Å and belongs to space group P3{sub 1} or P3{sub 2}. This crystal form is a merohedral twin with two molecules in the asymmetric unit and unit-cell parameters a = b = 74.70, c = 78.97 Å. Crystal form II, which was grown in the presence of Co{sup 2+}, diffracts to a resolution of 1.7 Å and belongs to space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 44.26, b = 81.99, c = 140.89 Å.

  19. 木瓜酸奶的加工工艺%Study on Process Technology of Papaya Yogurt

    Institute of Scientific and Technical Information of China (English)

    付红军

    2011-01-01

    The best craft for making papaya yogurt obtained through experiment was as follows, content of papaya slurry, 30%(m/m); white sugar consumption, 8%(m/m); Streptococcus thermophilus:Lactobacillus bulgaricus,1:1 (m':m); inoculum, 3%(m/m); fermentation temperature, 42℃; fermentation time, 6 h; compound stabilizer, CMC;PGA=0.30% :0.20%(m:m). Papaya yogurt prepared under these conditions was homogeneous, with exquisite taste and moderate sour.%通过对木瓜酸奶的加工工艺进行试验,得出其最优配方为木瓜浆质量分数30%,白砂糖用量(m/m,下同)8%,嗜热链球菌∶保加利亚乳杆菌(m∶m,下同)1.0∶1.0,接种量(m/m,下同)3%,发酵温度42℃,发酵时间6h,最佳复合稳定剂为羧甲基纤维素钠(CMC)(m/m,下同)∶海藻酸丙二醇酯(PGA)(m/m,下同)=0.30%∶0.20%,制得的木瓜酸奶组织均匀,口感细腻,酸甜适度.

  20. The component of Carica papaya seed toxic to A. aegypti and the identification of tegupain, the enzyme that generates it.

    Science.gov (United States)

    Nunes, Natalia N dos S; Santana, Lucimeire A; Sampaio, Misako U; Lemos, Francisco J A; Oliva, Maria Luiza

    2013-07-01

    As Aedes aegypti transmits the etiologic agents of both yellow and dengue fever; vector control is considered essential to minimise their incidence. The aim of this work was to identify the component of Carica papaya seed toxic to A. aegypti, and the identification of tegupain, the enzyme that generates it. Aqueous extracts (1%, w/v) of the seed tegument and cotyledon of C. papaya are not larvicidal isolately. However, a mixture of 17μgmL(-1) tegument extract and 27μgmL(-1) cotyledon extract caused 100% larval mortality in a bioassay. The mixture was no longer larvicidal after the tegument extract was pre-treated at 100°C for 10min. The enzyme tegupain efficiently hydrolysed the substrate Z-Phe-Arg-pNan (Km 58.8μM, Kcat 28020s(-1), Kcat/Km 5×10(8)M(-1) s(-1)), and its activity increased with 2mM dithiothreitol (DTT), at 37°C, pH 5.0. The chelating agent EDTA did not modify the enzyme activity. Inhibition of tegupain by cystatin (Kiapp 2.43nM), E64 (3.64nM, 83% inhibition), and the propeptide N-terminal sequence indicate that the toxic activity is due to a novel cysteine proteinase-like enzyme, rendered active upon the hydrolysis of a cotyledon component of C. papaya seeds.

  1. Current status of tropical fruit breeding and genetics for three tropical fruit species cultivated in Japan: pineapple, mango, and papaya

    Science.gov (United States)

    Ogata, Tatsushi; Yamanaka, Shinsuke; Shoda, Moriyuki; Urasaki, Naoya; Yamamoto, Toshiya

    2016-01-01

    Tropical fruit crops are predominantly produced in tropical and subtropical developing countries, but some are now grown in southern Japan. Pineapple (Ananas comosus), mango (Mangifera indica) and papaya (Carica papaya) are major tropical fruits cultivated in Japan. Modern, well-organized breeding systems have not yet been developed for most tropical fruit species. Most parts of Japan are in the temperate climate zone, but some southern areas such as the Ryukyu Islands, which stretch from Kyushu to Taiwan, are at the northern limits for tropical fruit production without artificial heating. In this review, we describe the current status of tropical fruit breeding, genetics, genomics, and biotechnology of three main tropical fruits (pineapple, mango, and papaya) that are cultivated and consumed in Japan. More than ten new elite cultivars of pineapple have been released with improved fruit quality and suitability for consumption as fresh fruit. New challenges and perspectives for obtaining high fruit quality are discussed in the context of breeding programs for pineapple. PMID:27069392

  2. Preparation and physicochemical evaluation of emulsified virgin coconut oil (VCO)-carica papaya extract concoction using Tween80

    Science.gov (United States)

    Omar, Hazreen; Zubairi, Saiful Irwan; Fadhilah, Mohd Faizulhelmi; Omar, Dzolkhifli; Asib, Norhayu

    2016-11-01

    Carica papaya is a member of the Caricaceae. Its leaves have been used in folk medicine for centuries. Recent studies have shown its beneficial effects as an anti-inflammatory agent (Owoyele et al 2008) and anti-tumour15 as well as antioxidant and wound healing properties7. The study has shown that the effect of carica papaya leaves juice intake also can accelerate the rate of increase in platelet count among the patients infected with dengue fever and dengue haemorrhagic fever18. With all the goodness of carica papaya leaves, a formulation with addition of virgin coconut oil (VCO) is produced to give an enhanced supplement beverage to market nowadays. Virgin coconut oil is well known as anti-oxidant4. The combination of these two substances gives a balance combination in healthy supplement. In recent years the application of emulsion is rapidly increasing in many fields such as cosmetics and paints. Emulsions are dispersions of droplets of one liquid in another, immiscible, liquid in which the droplets are of colloidal or near-colloidal sizes. The combination of water and oil (VCO) with addition of non-ionic surfactant Tween80 was constructed using ternary phase diagram. By considering the Hydrophilic-Lipophilic Balance (HLB) value of each substance will help in producing a stable emulsion.

  3. L-ascorbic acid, β-carotene and lycopene content in papaya fruits (Carica papaya with or without physiological skin freckles Conteúdo de ácido l-ascórbico, β-caroteno e licopeno em frutos de mamão (Carica papaya com e sem mancha fisiológica

    Directory of Open Access Journals (Sweden)

    Leandro Marelli de Souza

    2008-01-01

    Full Text Available The Skin Freckles is a papaya skin disorder that depreciates de fruit appearance and hampers its commercialization, although not lowering its nutritive value. Being the papaya a good source of ascorbic acid, β-carotene and licopene this research aimed at determining L-ascorbic acid, β-carotene and licopene content in papaya fruits, from 'Formosa' and 'Solo' group varieties, with and without apparent physiological skin disease (skin freckles. Fruits were harvested in the Southeast Region of Brazil. L-ascorbic acid content was determined by titration technique. β-carotene and licopene contents were determined by high performance liquid chromatography technique (HPLC. L-ascorbic acid content in papaya fruits ranged from (59.9 ± 3.4 mg 100 g-1 to (112.4 ± 12.6 mg 100 g-1 in fresh papaya pulp. β-carotene content ranged from (0.19 ± 0.07 mg 100 g-1 to (0.56 ± 0.09 mg 100 g-1 and that of licopene ranged from (1.44 ± 0.28 mg 100 g-1 to (3.39 ± 0.32 mg 100 g-1 in fresh papaya pulp. L-ascorbic acid contents of papaya fruits with skin disease averaged 7.0 mg 100 g-1 to 10.0 mg 100 g-1 higher than those of papaya fruits without skin freckles (P A Mancha Fisiológica do Mamão (MFM é uma desordem da casca do mamão, que deprecia a aparência do fruto e prejudica a sua comercialização, embora não prejudique o seu valor nutritivo. Considerando ser o mamão, uma boa fonte de ácido L-ascórbico, b-caroteno e licopeno, esta pesquisa visou determinar o índice destes componentes em frutas de mamão, das variedades do grupo 'Formosa' e 'Solo', com e sem Mancha Fisiológica do Mamão (MFM aparente na pele. As frutas foram colhidas na região do sudeste de Brasil. O teor de ácido L-ascórbico foi determinado pela técnica de titulação. Os índices do b-caroteno e do licopeno foram determinados pela técnica de cromatografia líquida de alta eficiência (HPLC. O teor de ácido L-ascórbico variou de (59,9 ± 3,4 mg 100 g-1 a (112,4 ± 12,6 mg 100 g-1

  4. Efecto de la madurez, geometría y presión sobre la cinética de transferencia de masa en la deshidratación osmótica de papaya (Carica papaya L., var. Maradol

    Directory of Open Access Journals (Sweden)

    Lina Marcela Chavarro-Castrillón

    2006-09-01

    Full Text Available Las papayas obtenidas en el raleo (papayas menos desarrolladas entresacadas de los árboles para beneficiar el crecimiento de las otras generalmente se descartan. Alternativamente, estas papayas pueden secarse por un proceso de deshidratación osmótica y secado térmico convencional para usarlas como snacks o como ingrediente para otros productos. Se comparó la cinética de transferencia de masa en la deshidratación osmótica de papayas de raleo frente a papayas desarrolladas con diferente grado de madurez, considerando el efecto de la geometría de la muestra y de la presión del proceso. La deshidratación osmótica se efectuó en una solución agitada de sacarosa a 50 °Brix, a 25 °C. Se consideraron tres niveles de madurez: raleo, verde y pintona, tres geometrías: lámina, cilindro y anillo y dos niveles de presión: atmosférica y vacío. Se estudiaron como variables cinéticas la variación de peso (WR, pérdida de agua (WL y ganancia de sólidos (SG entre 10 y 180 min. La madurez tuvo efecto significativo sobre las tres respuestas de la cinética a 30 min y sobre SG a 180 min; la geometría tuvo efecto sobre WR y SG a 30 min y sobre los tres parámetros cinéticos a 180 min y la presión solamente tuvo efecto sobre WL y SG a 30 min. La mayor pérdida de agua (65% se obtuvo a 30 min para la combinación raleo/cilindro/vacío; mientras que la mayor ganancia de sólidos fue 31% para el tratamiento verde/lámina/vacío a 180 min.

  5. Características fenotípicas y agronómicas de seis genotipos de papaya (Carica papaya L. de Tuxpan, Guerrero, México

    Directory of Open Access Journals (Sweden)

    José Ángel Alcántara Jiménez

    2010-07-01

    Full Text Available En México la producción de papayo (Carica papaya L. es afectada por factores genéticos, ambientales y bióticos. Destacan los daños provocados por el virus de la mancha anular, el cual reduce considerablemente la cantidad y calidad de la fruta; por eso es muy importante, conocer la productividad de los genotipos en un ambiente determinado. La presente investigación se realizó con el objetivo de evaluar el desarrollo y las características agronómicas de seis genotipos de papayo. El estudio se llevó a cabo en la localidad de Tuxpan, Guerrero, donde se cultivaron los genotipos: R4M3, Maradol, Criolla, Red Lady, Zapote y R5M2. Como diseño experimental se utilizó el de bloques completos al azar, con cuatro repeticiones. Las variables evaluadas fueron: altura al primer fruto, diámetro del cuello, altura de la planta, número de días a la cosecha, rendimiento de frutos (t/ha, número, diámetro y longitud de frutos, concentración de azúcares (grados Brix, textura, color de la pulpa y epicarpio de frutos maduros. Los datos de las variables se sometieron a análisis de varianza y prueba de comparación de medias de Tukey. En relación a la adaptación de los genotipos: todos los genotipos se desarrollaron y produjeron frutos en las condiciones de trópico seco. La variedad Maradol fue la más sobresaliente, presentando un porte menor, mayor precocidad y productividad, textura dura de pulpa, alta concentración de azúcares, buen tamaño y color.

  6. Características fenotípicas y agronómicas de seis genotipos de papaya (Carica papaya L. de Tuxpan, Guerrero, México

    Directory of Open Access Journals (Sweden)

    José Ángel Alcántara Jiménez

    2010-06-01

    Full Text Available En México la producción de papayo (Carica papaya L. es afectada por factores genéticos, ambientales y bióticos. Destacan los daños provocados por el virus de la mancha anular, el cual reduce considerablemente la cantidad y calidad de la fruta; por eso es muy importante, conocer la productividad de los genotipos en un ambiente determinado. La presente investigación se realizó con el objetivo de evaluar el desarrollo y las características agronómicas de seis genotipos de papayo. El estudio se llevó a cabo en la localidad de Tuxpan, Guerrero, donde se cultivaron los genotipos: R4M3, Maradol, Criolla, Red Lady, Zapote y R5M2. Como diseño experimental se utilizó el de bloques completos al azar, con cuatro repeticiones. Las variables evaluadas fueron: altura al primer fruto, diámetro del cuello, altura de la planta, número de días a la cosecha, rendimiento de frutos (t/ha, número, diámetro y longitud de frutos, concentración de azúcares (grados Brix, textura, color de la pulpa y epicarpio de frutos maduros. Los datos de las variables se sometieron a análisis de varianza y prueba de comparación de medias de Tukey. En relación a la adaptación de los genotipos: todos los genotipos se desarrollaron y produjeron frutos en las condiciones de trópico seco. La variedad Maradol fue la más sobresaliente, presentando un porte menor, mayor precocidad y productividad, textura dura de pulpa, alta concentración de azúcares, buen tamaño y color.

  7. 木瓜挤压破损力学特性研究%The study on mechanical properties of extruded damaged papaya

    Institute of Scientific and Technical Information of China (English)

    王涛; 艾星; 张喜瑞; 李粤; 梁栋

    2013-01-01

    为了解决木瓜运输过程中容易被压坏产生的损伤问题,利用电子万能试验机对木瓜进行压缩性能的试验研究。采用正交试验法研究木瓜受到破坏损伤的影响因素。选取了木瓜产生损伤的破坏力作为主要的试验指标,选取加载速度、加载方向、木瓜的成熟度为试验影响主要因素。结果表明:压缩速度为1.0mm/min、加载位置为宽的方向、成熟度为半成熟时,木瓜破碎所需加载力为最大。木瓜力学特性的试验研究不仅可以为木瓜的采摘机械化设计提供理论依据,也能为木瓜的运输、贮藏和加工提供基础。%Papaya transport process in order to solve easily crush injury problems, the use of electronic universal testing machine to test compression performance of papaya. By orthogonal test study papaya impact factors by the destruction of injury. Select a papaya produce in-jury destructive as the main indicators of the test, the loading speed is selected, the loading direction and papaya maturity test affect the main factors. The test results show that: the maturity is the papaya destructive influence of the most important factors. Compression speed is 1.0mm/min, the direction of the load position is wide, maturity of semi-mature, crushing load force required or maximum. The study of the papaya the mechanical characteristics of the test not only to provide a theoretical basis for papaya picking mechanization design, can provide the basis for the transportation, storage and processing off papaya.

  8. Metabolomic profiling of the phytomedicinal constituents of Carica papaya L. leaves and seeds by 1H NMR spectroscopy and multivariate statistical analysis.

    Science.gov (United States)

    Gogna, Navdeep; Hamid, Neda; Dorai, Kavita

    2015-11-10

    Extracts from the Carica papaya L. plant are widely reported to contain metabolites with antibacterial, antioxidant and anticancer activity. This study aims to analyze the metabolic profiles of papaya leaves and seeds in order to gain insights into their phytomedicinal constituents. We performed metabolite fingerprinting using 1D and 2D 1H NMR experiments and used multivariate statistical analysis to identify those plant parts that contain the most concentrations of metabolites of phytomedicinal value. Secondary metabolites such as phenyl propanoids, including flavonoids, were found in greater concentrations in the leaves as compared to the seeds. UPLC-ESI-MS verified the presence of significant metabolites in the papaya extracts suggested by the NMR analysis. Interestingly, the concentration of eleven secondary metabolites namely caffeic, cinnamic, chlorogenic, quinic, coumaric, vanillic, and protocatechuic acids, naringenin, hesperidin, rutin, and kaempferol, were higher in young as compared to old papaya leaves. The results of the NMR analysis were corroborated by estimating the total phenolic and flavonoid content of the extracts. Estimation of antioxidant activity in leaves and seed extracts by DPPH and ABTS in-vitro assays and antioxidant capacity in C2C12 cell line also showed that papaya extracts exhibit high antioxidant activity.

  9. Risk analysis of Papaya dieback disease introduction to China%番木瓜顶梢枯死病传入中国的风险分析

    Institute of Scientific and Technical Information of China (English)

    喻群芳; 张贺; 漆艳香; 陆英; 张欣; 张辉强; 谢艺贤; 蒲金基

    2014-01-01

    番木瓜顶梢枯死病(Papaya dieback disease)是番木瓜生产上最严重的病害之一。本文根据 FAO 国际植物检疫措施标准--检疫性有害生物风险分析( ISPM No.11),从番木瓜顶梢枯死病分布情况、进入可能性、定殖和扩散可能性、后果评估等方面对番木瓜顶梢枯死病传入中国开展风险分析。风险评估结果表明,番木瓜顶梢枯死病传入中国风险高,并提出了风险管理措施。%Papaya dieback disease is one of the most serious diseases of papaya production. According to the FAO’s“International standards for phytosanitary measures--Pest risk analysis for quarantine pests”(ISPMNO. 11),The risk of Papaya dieback disease introduction to China was assessed from geographical distribution, possibility of entry ,possibility of colonization and spreading ,consequence assessment. The result demonstrated that the risk of Papaya dieback disease introduction risk would be very high. Following the risk analysis, the methods of controlling the disease introducing to China were suggested.

  10. Does Carica papaya leaf-extract increase the platelet count? An experimental study in a murine model

    Institute of Scientific and Technical Information of China (English)

    Susiji Wickramasinghe; Roshitha Nilmini Waduge

    2013-01-01

    Objective:To investigate the potential role of fresh Carica papaya (C. papaya) leaf extract on haematological and biochemical parameters and toxicological changes in a murine model. Methods: In total 36 mice were used for the trial. Fresh C. papaya leaf extract [0.2 mL (2 g)/mouse] was given only to the test group (18 mice). General behavior, clinical signs and feeding patterns were recorded. Blood and tissue samples were collected at intervals. Haematological parameters including platelet, red blood cell (RBC), white blood cell (WBC), packed cell volume (PCV), serum biochemistry including serum creatinine, serum glutamic-oxaloacetic transaminase (SGOT) and serum glutamic-pyruvic transaminase (SGPT) were determined. Organs for possible histopathological changes were examined. Results: Neither group exhibited alteration of behavior or reduction in food and water intake. Similarly, no significant changes in SGOT, SGPT and serum creatinine levels were detected in the test group. Histopathological organ changes were not observed in either group of mice except in three liver samples of the test group which had a mild focal necrosis. The platelet count (11.33±0.35)í105/µL (P=0.000 04) and the RBC count (7.97±0.61)í106/µL (P=0.000 03) were significantly increased in the test group compared to that of the controls. However, WBC count and PCV (%) values were not changed significantly in the test group. The platelet count in the test group started to increase significantly from Day 3 (3.4±0.18í105/µL), reaching almost a fourfold higher at Day 21 (11.3í105/µL), while it was 3.8í105/µL and 5.5í105/µL at Day 3 and Day 21 respectively in the control. Likewise, the RBC count in the test group increased from 6í106/µL to 9í106/ µL at Day 21 while it remained near constant in the control group (6í106/µL). Conclusions: Fresh C. papaya leaf extract significantly increased the platelet and RBC counts in the test group as compared to controls. Therefore, it is very

  11. Orientación emprendedora y orientación de pequeña empresa en Chile y su impacto en el rendimiento de las PYMES chilenas

    Directory of Open Access Journals (Sweden)

    Ismael Oliva-Becerra

    2012-01-01

    Full Text Available Centrados en la Pequeña y Mediana Empresa chilena, se revisan los determinantes más importantes de la Orientación Emprendedora (OE: innovación, proclividad al riesgo y proactividad y la Orientación de Pequeña Empresa (OPE: involucramiento emocional y propósitos personales, como formulaciones distintas e identificables. Los resultados comprueban su independencia y su impacto positivo en el rendimiento organizacional, especialmente entre aquellos con un mayor grado de oportunismo. Sin embargo, en su conjunto, se demuestra que la Orientación de Pequeña Empresa (OPE, mejora el rendimiento en comparación con la Orientación Emprendedora (OE, lo cual se valida por la calificación de expertos, debido a factores culturales de los chilenos.

  12. ¿CUÁL ES EL CRECIMIENTO DE LARGO PLAZO DE LA ECONOMÍA CHILENA? UNA RESPUESTA FORMAL PARA UNA ANTIGUA PREGUNTA

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    Byron Idrovo

    2010-12-01

    Full Text Available El objetivo del presente estudio es estimar la tasa de crecimiento de la economía en un contexto de plena utilización de los recursos productivos. Se emplean para el análisis modelos univariados de series de tiempo, incluyendo una tendencia determinística (con quiebres de medias, y un modelo de estado-espacio que considera una tendencia estocástica y un componente cíclico, estimado por Filtro de Kalman. El componente cíclico es calculado con y sin cambios de régimen (Markov-switching models, de acuerdo con la idea de Friedman (1993 -plucking model. Los resultados indican que la tasa de crecimiento potencial de la economía chilena sería superior al 4% anual, acompañada de ciclos económicos asimétricos.

  13. ¿Cuál es el crecimiento de largo plazo de la economía chilena? Una respuesta formal para una antigua pregunta

    Directory of Open Access Journals (Sweden)

    Idrovo A. Byron

    2010-12-01

    Full Text Available El objetivo del presente estudio es estimar la tasa de crecimiento de la economía en un contexto de plena utilización de los recursos productivos. Se emplean para el análisis modelos univariados de series de tiempo, incluyendo una tendencia determinística (con quiebres de medias, y un modelo de estado-espacio que considera una tendencia estocástica y un componente cíclico, estimado por Filtro de Kalman. El componente cíclico es calculado con y sin cambios de régimen (Markov-switching models, de acuerdo con la idea de Friedman (1993 –plucking model. Los resultados indican que la tasa de crecimiento potencial de la economía chilena sería superior al 4% anual, acompañada de ciclos económicos asimétricos.

  14. Análisis de la implementación de programas de gestión del conocimiento en las empresas chilenas

    Directory of Open Access Journals (Sweden)

    Dario Liberona

    2013-01-01

    Full Text Available La gestión del conocimiento es una nueva disciplina que facilita la creación, el almacenamiento, la transfe- rencia y la aplicación de conocimientos en las organizaciones. En el caso de Chile no existe ningún estudio que aborde el estado de implementación de la gestión del conocimiento en las empresas y las dificulta- des para desarrollar estos programas. Este trabajo busca explorar el estado general de la implantación de estos programas en Chile y cuáles son las principales dificultades para desarrollarlos. Se identificaron 6 problemas principales para la adopción e implementación de programas de gestión del conocimiento, y se determina que si bien la gestión del conocimiento es conocida y valorada en las empresas chilenas, su adopción todavía es muy baja.

  15. El binomio identidad y cultura presentes en la historieta chilena Condorito. Aproximaciones conceptuales y análisis histórico social en la realidad nacional.

    Directory of Open Access Journals (Sweden)

    Alejandra Loreto González Hermosilla

    2013-12-01

    Full Text Available El siguiente artículo tiene el objetivo general de identificar los elementos del binomio identidad y cultura presentes en la historieta chilena de René Ríos “Condorito”. Se abordarán los conceptos claves para luego generar una aproximación en el contexto histórico social al que pertenecen. De este modo se podrá observar cómo la definición de  ́lo propio ́ se encuentra representada en la historieta, además de la visión de  ́lo chileno ́ en la propuesta de Condorito.

  16. Segregación residencial en las principales ciudades chilenas: Tendencias de las tres últimas décadas y posibles cursos de acción

    Directory of Open Access Journals (Sweden)

    Francisco Sabatini

    2001-12-01

    Full Text Available El patrón de segregación residencial de las ciudades chilenas se está transformando en dos sentidos principales: está cambiando su escala geográfica y está aumentando su malignidad. Estos cambios parecen estar afectando a la mayoría de las ciudades latinoamericanas. Están vinculados con las políticas de liberalización de los mercados de suelo y con los procesos de globalización económica y cultural de las últimas décadas. En el artículo se entrega información empírica sobre los efectos de estos cambios en tres ciudades chilenas (Santiago, Valparaíso, Concepción; y se discute teóricamente la relación entre desigualdad social y segregación espacial, rechazando el enfoque usual que ve a la segunda como un mero reflejo en el espacio de la primeraThe pattern of residential segregation is undergoing two crucial changes in Chilean cities: its geographical scale is shifting, and segregation’s malignancy is increasing. Seemingly, these changes are affecting most of Latin American cities. They are linked to the land markets’ liberalization policies and to the processes of economic and cultural globalization of the past decades. This paper presents empirical data showing the effects of these changes for three Chilean cities (Santiago, Valparaíso, Concepción; and theoretically discusses the relationship between social inequality and spatial segregation, rejecting the usual approach that considers the latter as a mere spatial reflection of the first

  17. RELACIÓN ENTRE LOS INSUMOS DEL PLAN ESTRATÉGICO Y LOS RESULTADOS PARA LA ACREDITACIÓN. CASO APLICADO A UNIVERSIDADES CHILENAS

    Directory of Open Access Journals (Sweden)

    Ana María Barra Salazar

    2014-01-01

    Full Text Available Es importante conocer el proceso de planificación estratégica que formulan las universidades, ya que les permite analizar los impactos en los resultados que obtienen, el logro de ciertos sellos diferenciadores y ventajas competitivas en relación a sus competidores, entre otros aspectos. Hoy en día los directivos universitarios deben reflexionar y cuestionarse cómo enfrentar este proceso y cuáles son las ventajas para una organización que lo posee. Es por esto que el objetivo de este artículo es analizar el grado de avance de los distintos insumos utilizados en la planificación estratégica y su relación con los resultados de las universidades en cuanto a acreditación, que les permitirán contar con una certificación de calidad en los procesos internos, y de este modo diferenciarse de otras instituciones de educación superior. Se utiliza como metodología la revisión bibliográfica de los términos relevantes del estudio, así como información secundaria de datos obtenidos de todas las universidades chilenas pertenecientes al Consorcio de Universidades Estatales Chilenas (CUECH. Luego, se definen criterios para cuantificar el grado de avance que presentan las universidades en relación a los in sumos involucrados en la formulación del plan estratégico, se analiza su relación con los resultados y se concluye que los planes estratégicos de las universidades están compuestos de distintos insumos y que el grado de avance en cuanto a su elaboración, n o tiene impacto en los resultados de las universidades.

  18. Índices de estacionalidad de los precios medios recibidos por los productores de manzanas chilenas Seasonal indices for mean prices received by Chilean apple farmers

    Directory of Open Access Journals (Sweden)

    Germán Lobos Andrade

    2005-11-01

    Full Text Available El objetivo de este trabajo fue analizar el comportamiento de los precios medios FOB (franco a bordo de manzanas frescas chilenas. A partir de este análisis, se estimaron los precios medios recibidos por los productores. Se estimaron patrones de estacionalidad ajustada de los precios recibidos por los productores de manzanas chilenas, a través del método del promedio geométrico móvil. Para las estimaciones se consideraron los precios FOB promedio mensuales desde enero de 1990 a septiembre de 2004. Los resultados mostraron: una baja estacionalidad de precios; una estabilidad de precios para junio y desde septiembre a diciembre; un valor máximo en julio. Los precios más bajos ocurrieron en abril y mayo. La principal conclusión, desde el punto de vista económico, sugiere que la rentabilidad de los huertos de manzanos podría ser incrementada, a través de un mejoramiento en el proceso de planificación de la producción.The purpose of this paper is to analyze the behavior of FOB (free on board average prices for the Chilean fresh apples. Based on this analysis, the average prices received by farmers were estimated. The patterns of seasonally adjusted price fluctuations, received by Chilean apple farmers, were estimated through average mobile geometric method. Estimates considered monthly average FOB prices from January 1990 up to September 2004. Results showed: a low seasonal price pattern; price stability for June and from September to December; the peak value in July. The lowest prices occurred in April and May. From an economic point of view, the main conclusion suggests that apple orchard profitability could be increased by an improved yield planning.

  19. Enclaves de la transición y democracia chilena Enclaves of the Transition and Chilean Democracy

    Directory of Open Access Journals (Sweden)

    PETER M SIAVELIS

    2009-01-01

    Full Text Available Algunas de las dificultades del gobierno de Michelle Bachelet y la crisis más generalizada del gobierno de la Concertación han estado cada vez más vinculadas a variables de liderazgo, al agotamiento de la coalición con respecto a sus ideales básicos, o a la necesidad de diversos tipos de reformas institucionales. Este trabajo contempla la posibilidad de que el modelo mismo de transición, que ha sido considerado como tan exitoso, podría ser el origen de las dificultades que hoy invaden tanto la Concertación como la democracia chilena en general. Además, argumenta que la interacción del sistema electoral y la estructura de la competencia postautoritaria han obligado a una política elitista que ayuda a explicar las dificultades actuales que sufre la Concertación, y ha forzado una crisis generalizada de representación. Para elaborar este argumento el autor se basa en el trabajo de Manuel Antonio Garretón, sobre los enclaves autoritarios, señalando que hay ciertos enclaves de la transición que dificultan el desarrollo de una democracia representativa de calidad. Estos enclaves de transición incluyen: el cuoteo, control de la élite en la selección de candidatos y la política electoral, dominación de los partidos en la política, formulación de políticas elitista y extrainstitucional, y la intocabilidad del modelo económico heredado del gobierno de Pinochet.Some of the difficulties of Michelle Bachelet Government and the more generalized crisis of Chile's governing Concertacián coalition have increasingly been tied to leadership variables, the exhaustion of the coalition with respect to its core ideals, or the need for various types of institutional reform. This paper considers the possibility that the very model of transition which has been lauded as so successful might actually be at the root of the difficulties that today plague both the Concertación and Chilean democracy more generally. This paper argues that the

  20. Construction of physical maps for the sex-specific regions of papaya sex chromosomes

    Directory of Open Access Journals (Sweden)

    Na Jong-Kuk

    2012-05-01

    Full Text Available Abstract Background Papaya is a major fruit crop in tropical and subtropical regions worldwide. It is trioecious with three sex forms: male, female, and hermaphrodite. Sex determination is controlled by a pair of nascent sex chromosomes with two slightly different Y chromosomes, Y for male and Yh for hermaphrodite. The sex chromosome genotypes are XY (male, XYh (hermaphrodite, and XX (female. The papaya hermaphrodite-specific Yh chromosome region (HSY is pericentromeric and heterochromatic. Physical mapping of HSY and its X counterpart is essential for sequencing these regions and uncovering the early events of sex chromosome evolution and to identify the sex determination genes for crop improvement. Results A reiterate chromosome walking strategy was applied to construct the two physical maps with three bacterial artificial chromosome (BAC libraries. The HSY physical map consists of 68 overlapped BACs on the minimum tiling path, and covers all four HSY-specific Knobs. One gap remained in the region of Knob 1, the only knob structure shared between HSY and X, due to the lack of HSY-specific sequences. This gap was filled on the physical map of the HSY corresponding region in the X chromosome. The X physical map consists of 44 BACs on the minimum tiling path with one gap remaining in the middle, due to the nature of highly repetitive sequences. This gap was filled on the HSY physical map. The borders of the non-recombining HSY were defined genetically by fine mapping using 1460 F2 individuals. The genetically defined HSY spanned approximately 8.5 Mb, whereas its X counterpart extended about 5.4 Mb including a 900 Kb region containing the Knob 1 shared by the HSY and X. The 8.5 Mb HSY corresponds to 4.5 Mb of its X counterpart, showing 4 Mb (89% DNA sequence expansion. Conclusion The 89% increase of DNA sequence in HSY indicates rapid expansion of the Yh chromosome after genetic recombination was suppressed 2–3 million years ago. The

  1. Effects of ozone exposure on `Golden' papaya fruit by photoacoustic phase-resolved method: Physiological changes associated with carbon dioxide and ethylene emission rates during ripening

    Science.gov (United States)

    Corrêa, Savio Figueira; Mota, Leonardo; Paiva, Luisa Brito; Couto, Flávio Mota do; Silva, Marcelo Gomes da; Oliveira, Jurandi Gonçalves de; Sthel, Marcelo Silva; Vargas, Helion; Miklós, András

    2011-06-01

    This work addresses the effects of ozone activity on the physiology of `Golden' papaya fruit. Depth profile analysis of double-layer biological samples was accomplished using the phase-resolved photoacoustic spectroscopy. The feasibility of the method was demonstrated by singling out the spectra of the cuticle and the pigment layers of papaya fruit. The same approach was used to monitor changes occurring on the fruit during ripening when exposed to ozone. In addition, one has performed real time studies of fluorescence parameters and the emission rates of carbon dioxide and ethylene. Finally, the amount of pigments and the changes in waxy cuticle have been monitored. Results indicate that a fruit deliberately subjected to ozone at a level of 6 ppmv underwent ripening sooner (at least 24-48 h) than a fruit stored at ambient conditions. Moreover, ozone caused a reduction in the maximum quantum yield of photosynthetic apparatus located within the skin of papaya fruit.

  2. La empresa educativa Chilena

    Directory of Open Access Journals (Sweden)

    Jenny Assaél Budnik

    2011-06-01

    Full Text Available Este artículo describe y analiza las características del llamado "experimento educativo chileno", tanto en sus aspectos estructurales, como de implementación, así como sus esenciales consecuencias. Finalmente se describen las principales tendencias generadas desde los movimientos sociales, que han implicado procesos de resistencia a las medidas neoliberales, así como una incipiente capacidad de propuesta alternativa. Para ello se revisaron un conjunto de fuentes primarias y secundarias de investigación sobre los alcances de este modelo. El artículo muestra que la aplicación sistemática de políticas educativas neoliberales es algo inédito en América Latina y que ha generado fenómenos y procesos sociales muy particulares, tanto de reproducción del modelo, como de resistencia social al mismo.

  3. Novel concepts on functional foods and nutrigenomics in healthy aging and chronic diseases: a review of fermented papaya preparation research progress

    Directory of Open Access Journals (Sweden)

    Francesco Marotta

    2012-05-01

    Full Text Available AbstractFunctional foods are an emerging research field corresponding with genomical, epidemiological and clinical studies integrated with the food industry in accordance with the consumer demands. Consequently, the features of the functional foods are being discussed by various researchers and related institutions, and a common view has been pointed out about the availability and the nature of the components of functional foods. Recently, the outcomes of functional foods are being assessed by the help of all the available scientific tools. Genomic medicine is one of the most promising areas of research to reveal the benefits of functional foods and the bioactive ingredients. Nutrigenomics aims at studying the genetic and epigenetic interactions with a nutrient or the functional component in order to lead to a phenotype change and therefore to the cell metabolism, differentiation or even apoptosis. Papaya and its fermentation product arespecific products derived from the technologically advanced and controlled environmentalfriendly bio-fermentation process. Functional Foods in Health and Disease 2012, 2(5:120-136 It has been well known for a long time that the natural anti-oxidant properties of papaya, mainly depending on vitamins A and C in addition to certain amino acids, were consistent both in the fruit and derived from the papain enzyme which is no longer present in the fermented product. In this article, functional foods in genomic medicine are discussed in review of the fermented papaya preparation research progress. Clinical evidences about fermented papaya as a functional food are reported as supported by various research protocols and experimental models. The benefits of fermented papaya preparation are also discussed in nutrigenomic basis and it is reported to have an important antioxidant and transcriptomic potential which deserves further investigation. As a conclusion, fermented papaya preparation represents a Functional Food

  4. Generation of hermaphrodite transgenic papaya lines with virus resistance via transformation of somatic embryos derived from adventitious roots of in vitro shoots.

    Science.gov (United States)

    Kung, Yi-Jung; Yu, Tsong-Ann; Huang, Chiung-Huei; Wang, Hui-Chin; Wang, Shin-Lan; Yeh, Shyi-Dong

    2010-08-01

    Papaya production is seriously limited by Papaya ringspot virus (PRSV) worldwide and Papaya leaf-distortion mosaic virus (PLDMV) in Eastern Asia. An efficient transformation method for developing papaya lines with transgenic resistance to these viruses and commercially desirable traits, such as hermaphroditism, is crucial to shorten the breeding program for this fruit crop. In this investigation, an untranslatable chimeric construct pYP08 containing truncated PRSV coat protein (CP) and PLDMV CP genes coupled with the 3' untranslational region of PLDMV, was generated. Root segments from different portions of adventitious roots of in vitro multiple shoots of hermaphroditic plants of papaya cultivars 'Tainung No. 2', 'Sunrise', and 'Thailand' were cultured on induction medium for regeneration into somatic embryos. The highest frequency of somatic embryogenesis was from the root-tip segments of adventitious roots developed 2-4 weeks after rooting in perlite medium. After proliferation, embryogenic tissues derived from somatic embryos were wounded in liquid-phase by carborundum and transformed by Agrobacterium carrying pYP08. Similarly, another construct pBG-PLDMVstop containing untranslatable CP gene of PLDMV was also transferred to 'Sunrise' and 'Thailand', the parental cultivars of 'Tainung No. 2'. Among 107 transgenic lines regenerated from 349 root-tip segments, nine lines of Tainung No. 2 carrying YP08 were highly resistant to PRSV and PLDMV, and 9 lines (8 'Sunrise' and 1 'Thailand') carrying PLDMV CP highly resistant to PLDMV, by a mechanism of post-transcriptional gene silencing. The hermaphroditic characteristics of the transgenic lines were confirmed by PCR with sex-linked primers and phenotypes of flower and fruit. Our approach has generated transgenic resistance to both PRSV and PLDMV with commercially desirable characters and can significantly shorten the time-consuming breeding programs for the generation of elite cultivars of papaya hybrids.

  5. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats

    Institute of Scientific and Technical Information of China (English)

    Hussein O B Oloyede; Matthew C Adaja; Taofeek O Ajiboye; Musa O Salawu

    2015-01-01

    OBJECTIVE:Carica papaya is an important fruit with its seeds used in the treatment of ulcer in Nigeria. This study investigated the anti-ulcerogenic and antioxidant activities of aqueous extract of Carica papaya seed against indomethacin-induced peptic ulcer in male rats. METHODS:Thirty male rats were separated into 6 groups (A–F) of ifve rats each. For 14 d before ulcer induction with indomethacin, groups received once daily oral doses of vehicle (distil ed water), cimetidine 200 mg/kg body weight (BW), or aqueous extract of C. papaya seed at doses of 100, 150 or 200 mg/kg BW (groups A, B, C, D, E and F, respectively). Twenty-four hours after the last treatment, groups B, C, D, E and F were treated with 100 mg/kg BW of indomethacin to induce ulcer formation. RESULTS:Carica papaya seed extract signiifcantly (P<0.05) increased gastric pH and percentage of ulcer inhibition relative to indomethacin-induced ulcer rats. The extract signiifcantly (P<0.05) decreased gastric acidity, gastric acid output, gastric pepsin secretion, ulcer index and gastric secretion volume relative to group B. These results were similar to that achieved by pretreatment with cimetidine. Speciifc activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase in the extract-treated groups (D, E and F) were increased signiifcantly over the group B (P<0.05). Pretreatment with the seed extract protected rats from the indomethacin-mediated decrease in enzyme function experienced by the group B. Similarly, indomethacin-mediated decrease in reduced glutathione level and indomethacin-mediated increase in malondialdehyde were reversed by Carica papaya extract. CONCLUSION:In this study, pretreatment with aqueous extract of Carica papaya seed exhibited anti-ulcerogenic and antioxidant effects, which may be due to the enhanced antioxidant enzymes.

  6. The use of natural product-papaya pulp powder as a disinte-grant in tablet formulation and their invitro evaluation

    Institute of Scientific and Technical Information of China (English)

    Dilip Chandrasekhar; Saraswathy R; Krishnan.N.V

    2009-01-01

    Objective:An attempt was made to study the use of papaya pulp powder as a disintegrant in tablet formula-tions.The objective of the present work is to identify a natural binding and disintegrating agent for formulating tablets and to study the effect of disintegrating agents and binding agents on the dissolution of the formulation containing paracetamol.Methods:Papaya pulp powder is obtained from unripe papaya fruit.The fruit was screened for its physical and chemical characteristics and used in tablet formulations.In order to find out the percentage that could be used to formulate a product containing good disintegrating and dissolution characteris-tics,several formulations (Paracetamol)with different concentrations of 8%,10%,12%,15%,20%,25% &30% were prepared.As a comparison,an already established disintegrant,sodium starchgylcolate was select-ed and several formulations containing similar concentrations,were also prepared.The invitro evaluation of the formulations were undertaken,and the results compared.In the present study preformulation studies on the pu-rity,development of calibration curve of the drug and the compatibility between the drug and excepients were carried out.The fruits were cut into small pieces,grated,dried and powdered,passed through different sieves and made into fine powder.Fine powder of papaya was mixed with required amount of drug and sodium starch-gylcolate individually in different concentrations along with other additives &binding agents.The dried gran-ules were compressed into tablets and all the formulated dosage forms of paracetamol tablets were subjected to quality control tests like hardness disintegration and dissolution.Results:From the results it was observed that formulations S1 and P7 containing 8% of sodium starchgylcolate and 30% of papaya pulp powder showed good disintegration and dissolution characteristics.Conclusion:Since the tablet formulation P7 containing 30% of papaya pulp powder shows good disintegration and

  7. Photoacoustic study of the influence of the cooling temperature on the CO2 emission rate by Carica papaya L. in modified atmosphere

    Science.gov (United States)

    Schramm, D. U.; Sthel, M. S.; da Silva, M. G.; Carneiro, L. O.; Silva, H. R. F.; Martins, M. L. L.; Resende, E. D.; Vitorazi, L.; Vargas, H.

    2005-06-01

    The monitoring of trace gas emitted by papaya fruits and assessments of its mass loss can contribute to improve the conditions for their storage and transport. The C02 emission rate by the papaya fruits, monitored by a commercial infrared-based gas analyzer, was influenced by the temperature and storage time. The fruits stored at temperature of 13 °C accumulated more CO2 inside the PEBD bags than those fruits stored at 6 °C. The loss of mass of the fruits progressively increased with storage time for both temperatures until the saturation of the moisture inside the PEBD bag, been more pronounced at 13 ºC.

  8. Glycyl endopeptidase from papaya latex: partial purification and use for production of fish gelatin hydrolysate.

    Science.gov (United States)

    Karnjanapratum, Supatra; Benjakul, Soottawat

    2014-12-15

    An aqueous two-phase system (ATPS) in combination with ammonium sulphate ((NH4)2SO4) precipitation was applied to fractionate glycyl endopeptidase from the papaya latex of Red Lady and Khack Dum cultivars. ATPS containing polyethylene glycol (PEG 2000 and 6000) and salts ((NH4)2SO4 and MgSO4) at different concentrations were used. Glycyl endopeptidase with high purification fold (PF) and yield was found in the salt-rich bottom phase of ATPS with 10%PEG 6000-10% (NH4)2SO4. When ATPS fraction from Red Lady cultivar was further precipitated with 40-60% saturation of (NH4)2SO4, PF of 2.1-fold with 80.23% yield was obtained. Almost all offensive odorous compounds, particularly benzyl isothiocyanate, were removed from partially purified glycyl endopeptidase (PPGE). The fish gelatin hydrolysates prepared using PPGE showed higher ABTS radical scavenging activity and less odour, compared with those of crude extract (CE). Thus antioxidative gelatin hydrolysate with negligible undesirable odour could be prepared with the aid of PPGE.

  9. Effect of wine inhibitors on the proteolytic activity of papain from Carica papaya L. latex.

    Science.gov (United States)

    Benucci, Ilaria; Esti, Marco; Liburdi, Katia

    2015-01-01

    The influence of potential inhibitors naturally present in wine on the proteolytic activity of papain from Carica papaya latex was investigated to evaluate its applicability in white wine protein haze stabilization. Enzymatic activity was tested against a synthetic tripeptide chromogenic substrate in wine-like acidic medium that consisted of tartaric buffer (pH 3.2) supplemented with ethanol, free sulfur dioxide (SO2 ), grape skin and seed tannins within the average ranges of concentrations that are typical in wine. The diagnosis of inhibition type, performed with the graphical method, demonstrated that all of tested wine constituents were reversible inhibitors of papain. The strongest inhibition was exerted by free SO2 , which acted as a mixed-type inhibitor, similar to grape skin and seed tannins. Finally, when tested in table white wines, the catalytic activity of papain, even when if it was ascribable to the hyperbolic behavior of Michaelis-Menten equation, was determined to be strongly affected by free SO2 and total phenol level.

  10. Characterization and occurence of eairly softening disorder in 'golden' papaya fruits

    Directory of Open Access Journals (Sweden)

    Angelo Pedro Jacomino

    2010-12-01

    Full Text Available The occurrence of green skin and soft pulp in 'Golden' papaya fruit during certain seasons has been reported by farmers in the northern of the state of Espirito Santo, Brazil. The objective of this study was to characterize and determine the occurrence of this disorder, which was referred as "early softening disorder". Fruits were harvested weekly for 11 months (from September to July. The fruits were stored at 10°C, and then fruit flesh firmness and skin color were analyzed. The results of the firmness test were submitted to regression analysis assuming a linear trendline. The slope of the curve was called the 'softening index' (SI. Fruits with early softening are characterized by a loss of firmness in less than 10 days, even when stored under refrigeration. Although softened, the skin of the fruit remains partially green. Fruits with the disorder occurred more frequently from mid-summer to mid-autumn (February to May. It is not possible to distinguish early softening disorder fruits from those without the disorder by skin color and flesh firmness analysis at the time of the harvest.

  11. Mapping the surface-exposed regions of papaya mosaic virus nanoparticles.

    Science.gov (United States)

    Rioux, Gervais; Majeau, Nathalie; Leclerc, Denis

    2012-06-01

    In general, the structure of the papaya mosaic virus (PapMV) and other members of the potexviruses is poorly understood. Production of PapMV coat proteins in a bacterial expression system and their self-assembly in vitro into nanoparticles is a very useful tool to study the structure of this virus. Using recombinant PapMV nanoparticles that are similar in shape and appearance to the plant virus, we evaluated surface-exposed regions by two different methods, immunoblot assay and chemical modification with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide or diethyl-pyrocarbonate followed by mass spectrometry. Three regions were targeted by the two techniques. The N- and C-termini were shown to be surfaced exposed as expected. However, the region 125-136 was revealed for the first time as the major surface-exposed region of the nanoparticles. The presence of linear peptides at the surface was finally confirmed using antibodies directed to those peptides. It is likely that region 125-136 plays a key role in the lifecycle of PapMV and other members of the potexvirus group.

  12. Characterisation of a virus from Australia that is closely related to papaya mosaic potexvirus.

    Science.gov (United States)

    Geering, A D; Thomas, J E

    1999-01-01

    We have isolated a previously undescribed potexvirus from Alternanthera pungens (Amaranthaceae) in southern Queensland, Australia. This virus was shown to have a moderately wide experimental host range, infecting plants in nine of the twelve families tested. Using specific antibodies, a plate trapped antigen ELISA was developed, allowing detection of virions down to 0.8 microgram/ml of leaf extract. Virions averaged 554 nm long and had a capsid protein with a M(r) of 23.1 x 10(3). A portion of the genome containing the capsid protein ORF and 3' untranslated region was cloned and sequenced. From both serological and amino acid sequence comparisons, the virus was shown to be closely related to papaya mosaic potexvirus (PMV). To determine the taxonomic status of the virus, we assessed variation in the amino acid sequence of capsid proteins of distinct species within the potexvirus genus, as well as variation between strains of the same virus. When the core region of the capsid proteins were compared, distinct species had a maximum of 62.2% sequence identity, whereas strains had a minimum of 88.8% identity. By comparison, the core region of the capsid proteins of the Alternanthera virus and PMV had 79.8% identity. We have concluded that the Alternanthera virus is a different species from PMV, and its relationship with PMV resembles that of potyvirus subgroup members.

  13. Crystal structure of the coat protein of the flexible filamentous papaya mosaic virus.

    Science.gov (United States)

    Yang, Shaoqing; Wang, Tao; Bohon, Jen; Gagné, Marie-Ève Laliberté; Bolduc, Marilène; Leclerc, Denis; Li, Huilin

    2012-09-14

    Papaya mosaic virus (PapMV) is a filamentous plant virus that belongs to the Alphaflexiviridae family. Flexible filamentous viruses have defied more than two decades of effort in fiber diffraction, and no high-resolution structure is available for any member of the Alphaflexiviridae family. Here, we report our structural characterization of PapMV by X-ray crystallography and cryo-electron microscopy three-dimensional reconstruction. We found that PapMV is 135Å in diameter with a helical symmetry of ~10 subunits per turn. Crystal structure of the C-terminal truncated PapMV coat protein (CP) reveals a novel all-helix fold with seven α-helices. Thus, the PapMVCP structure is different from the four-helix-bundle fold of tobacco mosaic virus in which helix bundling dominates the subunit interface in tobacco mosaic virus and conveys rigidity to the rod virus. PapMV CP was crystallized as an asymmetrical dimer in which one protein lassoes the other by the N-terminal peptide. Mutation of residues critical to the inter-subunit lasso interaction abolishes CP polymerization. The crystal structure suggests that PapMV may polymerize via the consecutive N-terminal loop lassoing mechanism. The structure of PapMV will be useful for rational design and engineering of the PapMV nanoparticles into innovative vaccines.

  14. Zucchini shoestring virus: a distinct potyvirus in the papaya ringspot virus cluster.

    Science.gov (United States)

    Ibaba, J D; Laing, M D; Gubba, A

    2016-08-01

    Zucchini shoestring virus (ZSSV) has been proposed to be a putative potyvirus in the papaya ringspot virus (PRSV) cluster, based on the sequence similarity of its coat protein to those of related potyviruses. ZSSV has been associated with the outbreak of a damaging disease of baby marrow (Cucurbita pepo L.) that had been observed throughout the province of KwaZulu-Natal, in the Republic of South Africa (RSA). We report the genome sequence of ZSSV, determined by next-generation sequencing of total RNA extracted from an infected baby marrow (Cucurbita pepo L.). The ZSSV genome is 10,295 nucleotides long excluding the poly(A) tail and displays a typical potyvirus organization. Algerian watermelon mosaic virus (AWMV; EU410442.1) was identified as the closest relative of ZSSV, sharing the highest nucleotide sequence identity of 65.68%. The nucleotide and amino acid sequence identity values for each protein support the differentiation of ZSSV as a member of a distinct species in the genus Potyvirus. This taxonomic position was also confirmed using the Pairwise Sequence Comparison online tool from the National Center for Biotechnology Information. Phylogenetic analysis of the polyprotein coding sequence of ZSSV grouped ZSSV together with AWMV and Moroccan watermelon mosaic virus, but in different clusters. ZSSV is the second cucurbit-infecting virus in the PRSV cluster present in RSA.

  15. Respiration rate of Golden papaya stored under refrigeration and with different controlled atmospheres

    Directory of Open Access Journals (Sweden)

    Derliane Ribeiro Martins

    2014-10-01

    Full Text Available Knowledge of the respiration rate during the storage is important in the evaluation of the post-harvest tools that preserve fruit quality, and also for the provision of information for the development of new packages. This work aimed to evaluate the respiration rate of 'Golden' Carica papaya stored under refrigeration and controlled atmosphere conditions. The fruit was kept at 13 °C in controlled atmospheres comprising three levels of O2 (20.8 %, 6 %, 3 % with a minimum level of CO2 (0.1 %; and three levels of CO2 (0.1 %, 6 %, 12 % with the lowest level of O2 (3 %. Measurements were taken at intervals of 5 days during the 30 days of storage. The mass loss and the peel color of the fruits were identified at the end of the storage period. The fruit maintained under 'normal' atmosphere conditions (20.8 % O2 and 0.1 % CO2 increased its respiration rate after the 10th day, reaching after 30 days 4.3 times the initial value. After 30 days in 3 % O2, the respiration rate was 2.9 times less than in the normal atmosphere. The decrease in respiration rate minimized the mass loss in fruit stored at 3 % O2, but it was unaffected by increasing levels of CO2.

  16. Block of Viral Movement in Papaya Mutant Plants Resistant to Papaya Ringspot Virus%番木瓜抗病突变体阻碍环斑病毒体内运转

    Institute of Scientific and Technical Information of China (English)

    周国辉; 李华平; 等

    2001-01-01

    应用RT-PCR一步法检测了PRSV Ys株系在感病番木瓜及其抗病突 变体植株体内的运转 动态,结果表明:在感病植株中,接种后48hr接种叶的未接种部位可检出病毒,第4天部分 接种叶柄可检出病毒,第6天植株各部位均能检出病毒;而在抗病植株中,接种后可以而且 仅能 在接种部位检出病毒;因而认为抗病突变体能够阻碍病毒从接种部位运出及(或)向未接种 部位运入。%One-step RT-PCR was used to detect papaya ringspot virus (PRSV ) moveme nt in susceptible and resistant (mutant) papaya (Carica papaya L.) plants. The t ime of post-inoculation at which PRSV was first detected in susceptible plants w as 48 hours in the other area of inoculated leaves out of inoculated sites , 4 d ays in the petioles of inoculated leaf and 6 days in all parts of plant. Compara tively, in resistant plants, the inoculated sites were the only area at which PR SV can be detected any time. Therefore, it was proposed that the mutated resista nce to PRSV protects the virus from moving out of inoculated sites or/and into non-inoculated areas.

  17. La enseñanza básica en Chile desde 1970 hasta 2009. Continuidades, cambiantes paradigmas e influencia extranjera en la escuela básica chilena

    DEFF Research Database (Denmark)

    Cancino, Rita

    La enseñanza básica en Chile desde 1970 hasta 2009: Continuidades, cambiantes paradigmas e influencia extranjera en la escuela básica chilena. Palabras clave: Filosofía educacional, enseñanza básica pública, paradigmas, pedagogía Resumen: En esta ponencia nos proponemos analizar la enseñanza básica...

  18. Biotic stress induced demolition of thylakoid structure and loss in photoelectron transport of chloroplasts in papaya leaves.

    Science.gov (United States)

    Nanda, Rashmi Madhumita; Biswal, Basanti

    2008-04-01

    Papaya mosaic virus (PMV) causes severe mosaic symptoms in the papaya (Carica papaya L.) leaves. The PMV-induced alterations in photosystem II (PS II) structure and photochemical functions were probed. An increase in chlorophyll a (Chl a) fluorescence polarization suggests pathogen-induced transformation of thylakoid membrane to a gel phase. This transformation in physical state of thylakoid membrane may result in alteration in topology of pigments on pigment-binding proteins as reflected in pathogen-induced loss in the efficiency of energy transfer from carotenoids to chlorophylls. The fast Chl a fluorescence induction kinetics of healthy and PMV-infected plants by F(O)-F(J)-F(I)-F(P) transients revealed pathogen-induced perturbation on PS II acceptor side electron transfer equilibrium between Q(A) and Q(B) and in the pool size of electron transport acceptors. Pathogen-induced loss in photosynthetic pigments, changes in thylakoid structure and decrease in the ratio of F(V)/F(M) (photochemical potential of PS II) further correlate with the loss in photoelectron transport of PS II as probed by 2,6-dichlorophenol indophenol (DCPIP)-Hill reaction. Restoration of the loss by 1,5-diphenyl carbazide (DPC), an exogenous electron donor, that donates electron directly to reaction centre II bypassing the oxygen evolving system (OES), leads towards the conclusion that OES is one of the major targets of biotic stress. Further, the data suggest that chlorophyll fluorescence could be used as a non-invasive handy tool to assess the loss in photosynthetic efficiency and symptom severity in infected green tissues vis-a-vis the healthy ones.

  19. Traditional Aboriginal Preparation Alters the Chemical Profile of Carica papaya Leaves and Impacts on Cytotoxicity towards Human Squamous Cell Carcinoma.

    Science.gov (United States)

    Nguyen, Thao T; Parat, Marie-Odile; Shaw, Paul N; Hewavitharana, Amitha K; Hodson, Mark P

    2016-01-01

    Carica papaya leaf decoction, an Australian Aboriginal remedy, has been used widely for its healing capabilities against cancer, with numerous anecdotal reports. In this study we investigated its in vitro cytotoxicity on human squamous cell carcinoma cells followed by metabolomic profiling of Carica papaya leaf decoction and leaf juice/brewed leaf juice to determine the effects imparted by the long heating process typical of the Aboriginal remedy preparation. MTT assay results showed that in comparison with the decoction, the leaf juice not only exhibited a stronger cytotoxic effect on SCC25 cancer cells, but also produced a significant cancer-selective effect as shown by tests on non-cancerous human keratinocyte HaCaT cells. Furthermore, evidence from testing brewed leaf juice on these two cell lines suggested that the brewing process markedly reduced the selective effect of Carica papaya leaf on SCC25 cancer cells. To tentatively identify the compounds that contribute to the distinct selective anticancer activity of leaf juice, an untargeted metabolomic approach employing Ultra High Performance Liquid Chromatography-Quadrupole Time of Flight-Mass Spectrometry followed by multivariate data analysis was applied. Some 90 and 104 peaks in positive and negative mode respectively were selected as discriminatory features from the chemical profile of leaf juice and >1500 putative compound IDs were obtained via database searching. Direct comparison of chromatographic and tandem mass spectral data to available reference compounds confirmed one feature as a match with its proposed authentic standard, namely pheophorbide A. However, despite pheophorbide A exhibiting cytotoxic activity on SCC25 cancer cells, it did not prove to be the compound contributing principally to the selective activity of leaf juice. With promising results suggesting stronger and more selective anticancer effects when compared to the Aboriginal remedy, Carica papaya leaf juice warrants further study

  20. Participación chilena en reuniones IADR mundiales, 2001-2012 Chilean participation in IADR general sessions, 2001-2012

    Directory of Open Access Journals (Sweden)

    R Cartes-Velásquez

    2013-04-01

    Full Text Available Introducción: Las reuniones mundiales de la International Association for Dental Research (IADR representan una de las instancias más importantes para la divulgación de las investigaciones odontológicas a nivel de congresos. El objetivo del presente trabajo es caracterizar la participación de investigadores de instituciones chilenas en reuniones IADR mundiales durante el período 2001-2012. Metodología: Estudio bibliométrico, la búsqueda se realizó en la plataforma web Conference Exchange™ para IADR, para las reuniones mundiales de 2001 a 2012 y el término libre "Chile", incluyendo todas las presentaciones con al menos un autor afiliado a una institución chilena; se consideraron las variables: Reunión, autor, institución, cooperación internacional y área temática. Resultados: Se hallaron 158 presentaciones, promedio de 13.2 (15.3 anuales, con participación de 1 a 6 autores en el 87.3%; la mayor participación corresponde a la Universidad de Chile (43%, seguida por las Universidades de Talca (15.8%, Finis Terrae (11.4% y de los Andes (10.8%; según área temática destaca Periodoncia (13.9%, Cariología (12.7% y Epidemiología (12.7%; 26% de las presentaciones tenía participación de instituciones extranjeras. Discusión: Los presentes resultados van en línea con reportes bibliométricos recientes en el área, aunque destaca la aparición de 2 universidades privadas en los primeros lugares. La valoración de la participación en las reuniones IADR mundiales representa un indicador valioso de la producción científica de la odontología chilena, que complementa las evaluaciones bibliométricas de publicaciones ISI, permitiendo una mejor comprensión de la generación de conocimiento en el área.Introduction: International Association for Dental Research (IADR general session is one of the most important annual congress in the dental and craniofacial field. The aim of this research is to characterize the participation of

  1. Efectos de la fertilización con nitrógeno en la producción de papaya (Carica papaya L. y en la incidencia de virosis.

    Directory of Open Access Journals (Sweden)

    Vallejo G. Gladys

    1999-06-01

    Full Text Available En un suelo Vertic Aplustoll arcilloso sobre franco esmetítico, isohipertérmico, del Centro de Producción Agropecuaria ACotové@, ubicado en el municipio de Santafé de Antioquia, en la zona de vida bosque seco Tropical (bs-T, se realizó un trabajo para determinar la respuesta de la variedad de papaya AU.N. Cotové@ (Carica papaya L. a la fertilización con N en dosis de 0, 183 y 366 kg/ha, equivalentes a dosis individuales de 5, 5, 10, 15, 25, 25, 25 y 10,10, 20, 30, 50, 50, 50 g de N/planta, respectivamente, fertilizando cada mes durante los primeros 4 meses después del transplante y cada 2 meses posteriormente, durante un período de 9 meses. Se suministraron además dosis constantes de P, K, S, Fe, Mn, Zn, y B. Se utilizó un diseño de bloques al azar con cuatro repeticiones y 10 plantas por unidad experimental, con distancias entre plantas de 2 m y entre surcos de 3m.

  2. A ¹H NMR Investigation of the Interaction between Phenolic Acids Found in Mango (Manguifera indica cv Ataulfo and Papaya (Carica papaya cv Maradol and 1,1-diphenyl-2-picrylhydrazyl (DPPH Free Radicals.

    Directory of Open Access Journals (Sweden)

    Luis M López-Martínez

    Full Text Available The benefits of phenolic acids on human health are very often ascribed to their potential to counteract free radicals to provide antioxidant protection. This potential has been attributed to their acidic chemical structure, which possesses hydroxyl groups in different positions. Phenolic acids can interact between themselves and exhibit an additive, antagonistic or synergistic effect. In this paper, we used 1H NMR to analyze the interactions and mechanisms that are present in major phenolic acids found in mango (gallic, protocatechuic, chlorogenic and vanillic acids and papaya (caffeic, ferulic and p-coumaric acids, and the DPPH radical was used to evaluate the effect of the antioxidant mixtures. The interactions were found to occur via hydrogen bonds between the -OH and -COOH groups. Moreover, the phenolic acids exhibit two types of mechanisms for the neutralization of the DPPH radical. According to the results, these two mechanisms are Hydrogen Atom Transfer (HAT and Single Electron Transfer (SET. The ability of the phenolic acid to neutralize the DPPH radical decreases in the following order in mango: gallic > chlorogenic > protocatechuic > vanillic. Moreover, within the acids found in papaya, the order was as follows: caffeic > p-coumaric > ferulic.

  3. A ¹H NMR Investigation of the Interaction between Phenolic Acids Found in Mango (Manguifera indica cv Ataulfo) and Papaya (Carica papaya cv Maradol) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) Free Radicals.

    Science.gov (United States)

    López-Martínez, Luis M; Santacruz-Ortega, Hisila; Navarro, Rosa-Elena; Sotelo-Mundo, Rogerio R; González-Aguilar, Gustavo A

    2015-01-01

    The benefits of phenolic acids on human health are very often ascribed to their potential to counteract free radicals to provide antioxidant protection. This potential has been attributed to their acidic chemical structure, which possesses hydroxyl groups in different positions. Phenolic acids can interact between themselves and exhibit an additive, antagonistic or synergistic effect. In this paper, we used 1H NMR to analyze the interactions and mechanisms that are present in major phenolic acids found in mango (gallic, protocatechuic, chlorogenic and vanillic acids) and papaya (caffeic, ferulic and p-coumaric acids), and the DPPH radical was used to evaluate the effect of the antioxidant mixtures. The interactions were found to occur via hydrogen bonds between the -OH and -COOH groups. Moreover, the phenolic acids exhibit two types of mechanisms for the neutralization of the DPPH radical. According to the results, these two mechanisms are Hydrogen Atom Transfer (HAT) and Single Electron Transfer (SET). The ability of the phenolic acid to neutralize the DPPH radical decreases in the following order in mango: gallic > chlorogenic > protocatechuic > vanillic. Moreover, within the acids found in papaya, the order was as follows: caffeic > p-coumaric > ferulic.

  4. A 1H NMR Investigation of the Interaction between Phenolic Acids Found in Mango (Manguifera indica cv Ataulfo) and Papaya (Carica papaya cv Maradol) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) Free Radicals

    Science.gov (United States)

    López-Martínez, Luis M.; Santacruz-Ortega, Hisila; Navarro, Rosa-Elena; Sotelo-Mundo, Rogerio R.; González-Aguilar, Gustavo A.

    2015-01-01

    The benefits of phenolic acids on human health are very often ascribed to their potential to counteract free radicals to provide antioxidant protection. This potential has been attributed to their acidic chemical structure, which possesses hydroxyl groups in different positions. Phenolic acids can interact between themselves and exhibit an additive, antagonistic or synergistic effect. In this paper, we used 1H NMR to analyze the interactions and mechanisms that are present in major phenolic acids found in mango (gallic, protocatechuic, chlorogenic and vanillic acids) and papaya (caffeic, ferulic and p-coumaric acids), and the DPPH radical was used to evaluate the effect of the antioxidant mixtures. The interactions were found to occur via hydrogen bonds between the -OH and -COOH groups. Moreover, the phenolic acids exhibit two types of mechanisms for the neutralization of the DPPH radical. According to the results, these two mechanisms are Hydrogen Atom Transfer (HAT) and Single Electron Transfer (SET). The ability of the phenolic acid to neutralize the DPPH radical decreases in the following order in mango: gallic > chlorogenic > protocatechuic > vanillic. Moreover, within the acids found in papaya, the order was as follows: caffeic > p-coumaric > ferulic. PMID:26559189

  5. A microsatellite library for Carica papaya L. cv. Sunrise solo Marcadores microssatélites para Carica papaya L. cv. Sunrise solo

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    Silvia Correa Santos

    2003-08-01

    Full Text Available In experimental areas of the Education and Researches Ilha Solteira and Jaboticabal UNESP/Campus farms were selected and tagged 20 hermaphrodite plants and 20 feminine of cultivar Sunrise Solo, Improved Sunrise Solo cv.72/12 and Baixinho of Santa Amália.The seeds origined of the selected fruits were cropped to be analysed the self-pollination efficiency and frequency of the sex in the progenies. After that, samples of the young leaf of the matrix plants were colected for the extration of the DNA. It was built five library enriched of microsatellite sequencies, using probes (TCA10, (TC13, (GATA4, (CAC10 e (TGAG8.It was possible the development of the primers only in the library that has utilized the probe (TCA10. This probe allowed the design of 32 primer pairs. From these, 31 presented pattern of unique band in agarose Metaphor and in acrilamide. For primer S36 were observed 2 bands, but with no polymorphism to differentiation in the sexual form at papaya tree culture. However, these primers can be tested, in the futures, in the investigation of the others features in segregated populations of this specie and the related species, germoplasm analysis, cultivars identification, parent evolution and molecular markers for the assisted plant breeding programs.Em áreas experimentais das Fazendas de Ensino e Pesquisa da UNESP/Campus de Ilha Solteira e Jaboticabal foram selecionadas e marcadas 20 plantas hermafroditas e 20 femininas dos cultivares Sunrise Solo, Improved Sunrise Solo cv.72/12 e Baixinho de Santa Amália. As sementes provenientes dos frutos selecionados foram plantadas para analisar-se a eficiência da autofecundação e a freqüência dos sexos nas progênies. Posteriormente, amostras de tecido foliar jovem das plantas matrizes foram coletadas para a extração de DNA. Foram construídas cinco bibliotecas enriquecidas de seqüências microsatélites, utilizando-se as sondas (TCA10, (TC13, (GATA4, (CAC10 e (TGAG8. Foi possível o

  6. Effectiveness of hydrothermal-calcium chloride treatment and chitosan on quality retention and microbial growth during storage of fresh-cut papaya.

    Science.gov (United States)

    Ayón-Reyna, Lidia E; Tamayo-Limón, Ransés; Cárdenas-Torres, Feliznando; López-López, Martha E; López-Angulo, Gabriela; López-Moreno, Héctor S; López-Cervántes, Jaime; López-Valenzuela, José A; Vega-García, Misael O

    2015-03-01

    Rapid degradation of fresh-cut papaya limits its marketability. Hydrothermal treatments in combination with a calcium dip, applied to whole fruit before slicing, and also the application of chitosan as a coating film, have been found to have very good results in maintaining the quality of fresh-cut fruits. Based on these considerations, the aim of this study was to evaluate the effect of hydrothermal treatment (HT; 49 °C, 25 min) containing calcium chloride (Ca; 1%, w/v) followed by dipping in chitosan (Chit; 1%, w/v, 3 min) on the physical, chemical, and microbial qualities of papaya slices stored at 5 °C for 10 d. Pulp color, firmness, ascorbic acid, total phenolics, β-carotene, and lycopene were evaluated every 2 d while the microbial quality (mesophilics, psychrophilics, molds, and yeasts) was evaluated every 5 d. Fruit treated with HT-Ca and HT-Ca + Chit showed better color and firmness retention than Control and Chit. Papaya slices treated with HT-Ca + Chit had higher nutritional content and lower microbial growth at the end of storage. The application of the HT-Ca + Chit could be used to reduce deterioration processes, maintaining physical, chemical, and microbial qualities and increasing the shelf life of fresh-cut papaya stored at 5 °C.

  7. Effect of dietary fiber on the bioaccessibility of phenolic compounds of mango, papaya and pineapple fruits by an in vitro digestion model

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    Gustavo VELDERRAIN-RODRÍGUEZ

    2016-01-01

    Full Text Available The presence of dietary fiber (DF in the food matrix of some tropical fruits plays an important role in the release and absorption of its bioactive compounds, such as phenolic compounds (PCs. The aim of this study was to evaluate the effect of the DF fractions in mango cv. ‘Ataulfo’, papaya cv. ‘Maradol’ and pineapple cv. ‘Esmeralda’, on the bioaccessibility of their PCs and antioxidant capacity (AOXC under an in vitro digestion model. The highest PCs content and AOXC was found in mango (274.30 mg GAE/100 g FW, followed by papaya (212 mg GAE//100 g FW, and pineapple (107.63 mg GAE/100 g FW, respectively. About 50% of the total PCs in all fruits was released at gastric phase, increasing closer to 60% at intestinal phase in mango and pineapple. However, the highest content of PCs associated to DF was found in mango (2.48 mg GAE/100 g FW compared with papaya DF fractions (0.96 GAE/100 g FW and pineapple (0.52 GAE/100 g FW. The presence of DF in mango, papaya and pineapple did not represent a major limitation on the bioaccessibility of its PCs according to the in vitro digestion model used in this study.

  8. Processamento mínimo de mamão 'formosa' Fresh cut of 'formosa' papaya

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    Gustavo Henrique de Almeida TEIXEIRA

    2001-01-01

    Full Text Available Este trabalho foi conduzido para verificar o efeito do tamanho do corte, 2,5 x 2,5cm (corte 1 e 2,5 x 5,0cm (corte 2, e da temperatura de armazenamento (3, 6 e 9ºC, na velocidade da modificação da atmosfera ambiente e nas características químicas de mamões do grupo `Formosa' minimamente processados e embalados em copos plásticos (500ml. A concentração de CO2 no interior destes copos aumentou 2 a 3 vezes, durante as primeiras 6 horas após o corte, para depois diminuir e se estabilizar. Esta concentração aumentou com o aumento da temperatura de armazenamento. A umidade dos pedaços diminuiu consideravelmente nos dois primeiros dias, e a temperatura que melhor conservou a umidade foi a de 6ºC. A acidez total titulável foi menor no corte 2, com as maiores reduções a 6 e 9oC. Os teores de sólidos solúveis totais não variaram entre os tratamentos, e os cuidados higiênicos adotados durante o processamento permitiram a obtenção de produtos com baixa contagem microbiana, 10³UFC.g-1 nos pedaços armazenados a 9ºC após sete dias, e com boa manutenção da qualidade sensorial das mesmas. Estes resultados permitem indicar o mamão `Formosa' para a produção de produtos minimamente processados, na forma de pedaços, com conservação refrigerada (3 e 6ºC por um período de 7 dias.This work was undertaken to verify the effect of cutting size, 2.5 x 2.5cm (chunk 1, 2.5 x 5.0cm (chunk 2, and storage temperatures (3, 6 and 9ºC, on atmosphere modification rate and chemical characteristics of `Formosa' fresh-cut papaya packed in plastic cups (500 ml. The CO2 concentration into theses cups increased fron two to three fold during first 6 hours after cutting, then it decreased and stabilized. This concentration increased with the storage temperature increasing. The chunks's moisture remarkably decreased in the first 2 days, and the temperature which best sustained the moisture was 6ºC. The titratable total acidity was lower in chunk 2

  9. "Matar al padre": Análisis discursivo de dos textos de la sociología chilena en período de dictadura

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    Miguel Alvarado

    2003-01-01

    Full Text Available Este artículo presenta una visión panorámica de las transformaciones de la sociología chilena durante el período de dictadura militar. Específicamente, desde una perspectiva lingüística se comenta el discurso escrito de la sociología chilena durante el período 1975-1989, tiempo durante el cual la producción escrita de esta disciplina se reconstituye bajo la influencia de las transformaciones del contexto sociocultural nacional, latinoamericano y mundial. Nuestra hipótesis de trabajo consiste en que dicha redefinición significó para estos discursos el paso desde un canon centrado en el concepto de la estructura social, hacia otro que enfatiza el concepto de cultura, asumida ésta como un sistema de valores. Lo anterior se demuestra por medio del análisis de dos textos fundamentales del período: Cultura y modernización en América Latina, de Pedro Morandé y El espejo trizado de José Joaquín Brünner.This article presents a panoramic view of the metamorphosis of Chilean sociology during the period of military dictatorship (1973-1989. The production of texts in Chilean sociology for the purpose of this investigation will be approached from a linguistic perspective. The written production of this discipline in this particular moment in history 1975-1989, demonstrates a reconstruction under the influence of the national, Latin American and international sociocultural context. Our work hypothesis is that the reconstruction of discipline oriented discourse meant that there was a movement from the general rule of viewing the social structure as the object of study towards one that emphasized culture, and accepting it as a system of values. The prior statement is demonstrated through the analysis of two texts of the period: Culture and Modernization by Pedro Morandé and The Broken Mirror by José Joaquín Brünner

  10. EL INGRESO DE LA MUJER CHILENA A LA UNIVERSIDAD Y LOS CAMBIOS EN LA COSTUMBRE POR MEDIO DE LA LEY 1872-1877

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    KARIN SÁNCHEZ MANRÍQUEZ

    2006-12-01

    Full Text Available El artículo reconstruye el proceso que llevó a la firma del llamado "Decreto Amunátegui", que permitió el ingreso de la mujer chilena a la universidad, y estudia la polémica que causó esta medida en la opinión pública. Para ello, se da cuenta de los intentos de dos directoras de colegios secundarios femeninos, Antonia Tarragó e Isabel Le Brun (en 1872 y 1876, respectivamente, porque sus alumnas dieran exámenes válidos para entrar a la Universidad de Chile. Los comentarios de sus infructuosos intentos en la prensa crearon el ambiente necesario para que el Ministro de Instrucción Pública, Miguel Luis Amunátegui, firmara, en febrero de 1877, el decreto que facilitaba a las mujeres el ingreso a la educación superior. Se estudia el significado de esta medida en el contexto de la secularización que experimentaba la sociedad chilena, lo cual se aprecia en la polémica desatada entre la prensa católica y la prensa liberal luego de la firma del mencionado decreto. Se concluye que esta medida refleja la tensión modernidad / tradición que caracteriza a la segunda mitad del siglo XIX chilenoThis article reconstructs the process that culminated in the "Decreto Amunátegui", which allowed Chilean women the application to university studies. It analyzes the polemics which took place in the country's public opinion, starting with the initiative by Antonia Tarragó and Isabel Le Brun (1872 and 1876 respectively for the recognition of high school exams as valid to apply to the Universidad de Chile. Press commentaries on their unsuccessful efforts contributed to building up the political environment for the signature, in February, 1877, by Miguel Luis Amunátegui, Minister of Education, of the decree facilitating the admittance of women to higher education. This article reflects on the significance of this decree in the context of the underlying secularization process, by paying special attention to the conflict expressed in the positions

  11. HERANÇA DA RESISTÊNCIA AO Papaya ringspot virus EM MELANCIA

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    LINDOMAR MARIA DA SILVEIRA

    2015-01-01

    Full Text Available Aiming to study the genetic control of Papaya ringspot virus, type watermelon (PRSV-W in watermelon, the cultivar Crimson Sweet (P1 – susceptible and L26 derived from PI 244019 (P2 – resistant, as well as the resulting populations F1, F2, RC11 and RC21 of the cross of both lines were evaluated. The trials were carried out in a greenhouse, and the evaluations were done using artificial inoculations with PRSV-W isolates. The seedling symptoms were recorded using a graded scale, and the serological evaluation was done with specific antiserum using indirect ELISA. The estimated variances of the populations were used to obtain the genetic (σ 2 G, the environmental (σ 2 E, phenotypic (σ 2 F2, additive (σ 2 A and dominance (σ 2 D variances as well as the broad (h2 a and narrow sense (h2 r heritabilities. The hypothesis of monogenic inheritance was tested under different presumed average degrees of dominance as well as using the maximum likelihood. The distribution of resistant plants in the segregating populations was different from a distribution based on monogenic inheritance for all presumed average degrees of dominance, therefore, the hypothesis of monogenic inheritance was rejected indicating that this character in the line L26 is controlled by more than one major gene with the presence of modifiers. The additive-dominant model was adequate to explain the type of gene action involved, and the epistatic effects were not important in the expression of the resistance. The estimated average degree of dominance indicated complete dominance. The broad sense heritabilities for the two variables analyzed were intermediate.

  12. Purification and biochemical characterization of a monomeric form of papaya mosaic potexvirus coat protein.

    Science.gov (United States)

    Lecours, Katia; Tremblay, Marie-Hélène; Gagné, Marie-Eve Laliberté; Gagné, Stéphane M; Leclerc, Denis

    2006-05-01

    Papaya mosaic virus (PapMV) is a flexuous rod shape virus made of 1400 subunits that assemble around a plus sense genomic RNA. The structure determination of PapMV and of flexuous viruses in general is a major challenge for both NMR and X-ray crystallography. In this report, we present the characterization of a truncated version of the PapMV coat protein (CP) that is suitable for NMR study. The deletion of the N-terminal 26 amino acids of the PapMV CP (CP27-215) generates a monomer that can be expressed to high level and easily purified for production of an adequate NMR sample. The RNA gel shift assay showed that CP27-215 lost its ability to bind RNA in vitro, suggesting that the multimerization of the subunit is important for this function. The fusion of a 6x His tag at the C-terminus improved the solubility of the monomer and allowed its concentration to 0.2 mM. The CD spectra of the truncated and the wild-type proteins were similar, suggesting that both proteins are well ordered and have a similar secondary structure. CP27-215 was 15N labeled for NMR studies and a 2D 1H-15N-HSQC spectrum confirmed the presence of a well-ordered structure and the monomeric form of the protein. These results show that CP27-215 is amenable to a complete and exhaustive NMR study that should lead to the first three-dimensional structure determination of a flexuous rod shape virus.

  13. Generation of transgenic watermelon resistant to Zucchini yellow mosaic virus and Papaya ringspot virus type W.

    Science.gov (United States)

    Yu, Tsong-Ann; Chiang, Chu-Hui; Wu, Hui-Wen; Li, Chin-Mei; Yang, Ching-Fu; Chen, Jun-Han; Chen, Yu-Wen; Yeh, Shyi-Dong

    2011-03-01

    Zucchini yellow mosaic virus (ZYMV) and Papaya ringspot virus type W (PRSV W) are major limiting factors for production of watermelon worldwide. For the effective control of these two viruses by transgenic resistance, an untranslatable chimeric construct containing truncated ZYMV coat protein (CP) and PRSV W CP genes was transferred to commercial watermelon cultivars by Agrobacterium-mediated transformation. Using our protocol, a total of 27 putative transgenic lines were obtained from three cultivars of 'Feeling' (23 lines), 'China baby' (3 lines), and 'Quality' (1 line). PCR and Southern blot analyses confirmed that the chimeric construct was incorporated into the genomic DNA of the transformants. Greenhouse evaluation of the selected ten transgenic lines of 'Feeling' cultivar revealed that two immune lines conferred complete resistance to ZYMV and PRSV W, from which virus accumulation were not detected by Western blotting 4 weeks after inoculation. The transgenic transcript was not detected, but small interfering RNA (siRNA) was readily detected from the two immune lines and T(1) progeny of line ZW 10 before inoculation, indicating that RNA-mediated post-transcriptional gene silencing (PTGS) is the underlying mechanism for the double-virus resistance. The segregation ratio of T(1) progeny of the immune line ZW10 indicated that the single inserted transgene is nuclearly inherited and associated with the phenotype of double-virus resistance as a dominant trait. The transgenic lines derived from the commercial watermelon cultivars have great potential for control of the two important viruses and can be implemented directly without further breeding.

  14. 提取因素对木瓜籽油提取率的影响%Effect of Extracting Factors on Extraction Rate of Papaya Seed Oil

    Institute of Scientific and Technical Information of China (English)

    张海涛; 张海容; 徐撒撒

    2012-01-01

    [Objective] The objective was to study the effects of different factors on the extraction rate of papaya seed oil, and confirm the optimum the technology conditions for extracting the papaya seed oil. [Method] With the papaya seed in the market-sold shine skin papaya as the tested material, the papaya seed oil was extracted by Soxhlet extraction method. The effects of the different extraction time, liquid-material ratio and extraction temperature on the extraction rate of the papaya seed oil were discussed and the optimum the conditions for extracting the papaya seed oil were confirmed through the single factor test and the orthogonal test. [ Result ] The single factor test showed that the optimum the extraction time for extracting the papaya seed oil was 9 h, the optimum liquid-material ratio was 8:1 and the optimum extraction temperature was 45 ℃. The orthogonal experiments based on the single factor test indicated that, the optimum the conditions for extracting the papaya seed oil were as follows; the extraction time of 9 h, liquid-material ratio of 7:1 and the extraction temperature of 48 ℃, under which, the oil yield of the papaya seed was 28.77%. [Conclusion] The study provided the reference basis for the comprehensive utilization and the development of the new products.%[目的]研究不同因素对木瓜籽油提取率的影响,确定木瓜籽油提取的最佳工艺条件.[方法]以市售光皮木瓜中的木瓜籽为试材,采用索式提取法提取木瓜籽油,通过单因素和正交试验,探讨不同的提取时间、液料比和提取温度对木瓜籽油提取率的影响,确定木瓜籽油提取的最佳条件.[结果]单因素试验表明,木瓜籽油提取的最佳提取时间为9h,最佳液料比为8:1,最佳提取温度为45℃.在此基础进行的正交试验表明,木瓜籽油提取的最佳提取条件为:提取时间9h、液料比7:1、提取温度为48℃,在此条件下,木瓜籽的出油率为28.77%.[结论]该研究为木瓜

  15. Safety Evaluation of Oral Toxicity of Carica papaya Linn. Leaves: A Subchronic Toxicity Study in Sprague Dawley Rats

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    Zakiah Ismail

    2014-01-01

    Full Text Available The subchronic toxicity effect of the leaf extract of Carica papaya Linn. in Sprague Dawley (SD rats was investigated in this study. The extract was prepared by dissolving the freeze dried extract of the leaves in distilled water and was administered orally to SD rats (consisted of 10 rats/sex/group at 0 (control, 0.01, 0.14, and 2 g/kg body weight (BW for 13 weeks. General observation, mortality, and food and water intake were monitored throughout the experimental period. Hematological and biochemical parameters, relative organ weights, and histopathological changes were evaluated. The study showed that leaf extract when administered for 13 weeks did not cause any mortality and abnormalities of behavior or changes in body weight as well as food and water intake. There were no significant differences observed in hematology parameters between treatment and control groups; however significant differences were seen in biochemistry values, for example, LDH, creatinine, total protein, and albumin. However, these changes were not associated with histopathological changes. In conclusion, the results suggested that daily oral administration of rats with C. papaya leaf extract for 13 weeks at a dose up to fourteen times the levels employed in traditional medicine practice did not cause any significant toxic effect.

  16. New Cultivars Derived from Crosses between Commercial Cultivar and a Wild Population of Papaya Rescued at Its Center of Origin

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    Mariela Vázquez Calderón

    2014-01-01

    Full Text Available In order to generate new varieties, this study focused on the rescue and use of landraces and wild Carica papaya L. populations located at southern Yucatan, Mexico, to cross them with a commercial papaya cultivar (Maradol. In the cross L7 × M22, The native parent line L7 was used as the receiver parent while the commercial Maradol (M22 was used as the donor parent, seeking to generate genotypes with improved productivity and reduced plant height. Cluster analysis and principal components analysis grouped the genotypes firstly into those individuals with few fruits and those with many fruits and secondly into individuals with high and low plant height. Selected genotypes H13B, H17B, H19B, H68B, and H71B meet the desirable characteristics, such as reduced plant height (PH and intermediate number of fruits per plant (NFP. These materials can be used now to produce new crosses to continue with the ongoing breeding program at CICY, seeking new varieties with higher productivity and adequate plant height, and also these genotypes will be preserved and integrated in the germplasm bank in situ and in vitro for further genetic work and possible exchange with other germplasm collections worldwide.

  17. Single- and double-stranded viral RNAs in plants infected with the potexviruses papaya mosaic virus and foxtail mosaic virus.

    Science.gov (United States)

    Mackie, G A; Johnston, R; Bancroft, J B

    1988-01-01

    Three classes of viral RNA were recovered from polyribosomes purified from papaya leaves infected with papaya mosaic virus (PapMV) and from barley leaves infected with foxtail mosaic virus (FoMV): full-length viral RNAs [6.8 and 6.2 kilobases (kb), respectively]; less abundant intermediate subgenomic RNAs (2.2 and 1.9 kb), and abundant, small subgenomic RNAs (1 and 0.9 kb). Small amounts of the PapMV-specified 1.0-kb subgenomic RNA were encapsidated, whereas no encapsidated subgenomic RNAs could be found in preparations of FoMV. Immunoprecipitation of the products of in vitro translation of the small subgenomic RNA of both viruses showed that it codes for the corresponding viral coat protein. FoMV genomic RNA isolated from polyribosomes also directed the efficient synthesis of a 37- to 38-kilodalton protein which was immunoprecipitated by an antiserum raised against the coat protein. We presume this product to be a readthrough protein initiated to the 5' side of and in the same reading frame as the coat protein-coding sequences in FoMV RNA. The predominant double-stranded viral-specified RNAs in tissues infected with PapMV, FoMV, and clover yellow mosaic virus were genome sized (6.8, 6.2, and 7.0 kb pairs, respectively). If double-stranded RNAs corresponding to coat protein subgenomic RNAs exist, they must be present in much lower relative abundances.

  18. Zucchini tigré mosaic virus is a distinct potyvirus in the papaya ringspot virus cluster: molecular and biological insights.

    Science.gov (United States)

    Romay, G; Lecoq, H; Desbiez, C

    2014-02-01

    In recent years, three new potyviruses have been described in the papaya ringspot virus (PRSV) cluster. In addition, two types of PRSV are recognized, type W, infecting cucurbit plants, and type P, infecting papaya and also cucurbits. A third type, PRSV-T, was also partially described in Guadeloupe. Complete genome sequencing of four PRSV-T isolates showed that this virus is a related virus that is distinct from PRSV, and the name zucchini tigré mosaic virus (ZTMV) is proposed, in reference to the typical symptoms observed in zucchini squash. Eleven other viral isolates from different geographic origins were confirmed as ZTMV isolates using the complete sequence of the cylindrical inclusion (CI) coding region, whereas pairwise sequence similarities in the coat protein (CP) coding region did not unambiguously distinguish ZTMV isolates from PRSV isolates. The use of the CI coding region for species demarcation appears more suitable than the CP coding region for closely related viruses. Principal coordinates analysis based on the biological behavior of the viral isolates studied clustered PRSV-P, PRSV-W and ZTMV isolates into three different groups. Therefore, ZTMV is different from PRSV in its molecular and biological properties.

  19. Preliminary in vitro antisickilng properties of crude juice extracts of Persia Americana, Citrus sinensis, Carica papaya and Ciklavit®.

    Science.gov (United States)

    Iweala, E E J; Uhegbu, F O; Ogu, G N

    2009-12-30

    The antisickling properties of crude juice extracts of the edible portions of three commonly consumed tropical fruits namely Persia americana, Citrus sinensis, and Carica papaya were investigated in vitro alongside a new drug preparation called Ciklavit® that has antisickling activity. Four different solvent extracts of the crude juice of each fruit including aqueous, acidic, alkaline and alcoholic extracts were prepared and their antisickling effects on sickle cell trait (HbAS) and sickle cell disease (HbSS) blood samples checked alongside Ciklavit®. Blood samples were stabilized using normal saline and the antisickling effects were checked by counting the number of sickle cells remaining after incubation of the blood samples with the crude fruit extracts and Ciklavit® for twenty-four hours. The results showed that Ciklavit® produced a sustained reduction in the number of sickle cells in both HbAS and HbSS blood samples. Also the alkaline and alcoholic extracts of P. americana and C. papaya produced significant reduction in the number of sickle cells.

  20. Papaya Seeds as A Low-Cost Sorbent for Removing Cr(VI) from The Aqueous Solution

    Science.gov (United States)

    Rahmawati, Atik; Marwoto, Putut; Karunia Z, Anita

    2016-08-01

    The presence of chromium (VI) contaminants and their toxicity in aqueous streams important environmental problems. Adsorption is one of the effective techniques that can be used for removing metal from wastewater. This research was initiated by preparing sorbent from papaya seeds and determining its functional group contents by using FT-IR. The adsorption process was carried out in a batch method. The study of adsorption aspects involved the pH, initial Cr (VI) concentration and contact time between Cr (VI) and sorbent. FT IR analysis results showed that the main functional groups are carbonyl, hydroxyl, and carboxylic. It was also found that the effective pH for Cr (VI) uptake is 2.0 and increasing contact time would increase the Cr (VI) uptake. In addition, the equilibrium was reached after 40 minutes interaction and the increase of initial chromium (VI) concentration would increase the sorbent uptake percentage. All these results indicated that papaya seed is a potential sorbent for removing Cr (VI) from aqueous solutions.

  1. Crystallization and preliminary X-ray analysis of a family 19 glycosyl hydrolase from Carica papaya latex

    Energy Technology Data Exchange (ETDEWEB)

    Huet, Joëlle, E-mail: jhuet@ulb.ac.be [Laboratoire de Chimie Générale (CP 206/4), Institut de Pharmacie, Université Libre de Bruxelles (ULB), Campus de la Plaine, Boulevard du Triomphe, B-1050 Bruxelles (Belgium); Azarkan, Mohamed [Laboratoire de Chimie Générale (CP 609), Faculté de Médecine, Université Libre de Bruxelles (ULB), Campus Erasme, 808 Route de Lennik, B-1070 Bruxelles (Belgium); Looze, Yvan [Laboratoire de Chimie Générale (CP 206/4), Institut de Pharmacie, Université Libre de Bruxelles (ULB), Campus de la Plaine, Boulevard du Triomphe, B-1050 Bruxelles (Belgium); Villeret, Vincent [CNRS-UMR 8161, Institut de Biologie de Lille, Université de Lille 1-Université de Lille 2-Institut Pasteur de Lille, IFR142, 1 Rue du Professeur Calmette, F-59021 Lille (France); Wintjens, René, E-mail: jhuet@ulb.ac.be [Laboratoire de Chimie Générale (CP 206/4), Institut de Pharmacie, Université Libre de Bruxelles (ULB), Campus de la Plaine, Boulevard du Triomphe, B-1050 Bruxelles (Belgium)

    2008-05-01

    A chitinase isolated from the latex of the tropical species Carica papaya has been crystallized. The addition of N-acetyl-d-glucosamine to the crystallization solution has improved the diffraction quality resolution of the crystal to 1.8 Å resolution. A chitinase isolated from the latex of the tropical species Carica papaya has been purified to homogeneity and crystallized. This enzyme belongs to glycosyl hydrolase family 19 and exhibits exceptional resistance to proteolysis. The initially observed crystals, which diffracted to a resolution of 2.0 Å, were improved through modification of the crystallization protocol. Well ordered crystals were subsequently obtained using N-acetyl-d-glucosamine, the monomer resulting from the hydrolysis of chitin, as an additive to the crystallization solution. Here, the characterization of a chitinase crystal that belongs to the monoclinic space group P2{sub 1}, with unit-cell parameters a = 69.08, b = 44.79, c = 76.73 Å, β = 95.33° and two molecules per asymmetric unit, is reported. Diffraction data were collected to a resolution of 1.8 Å. Structure refinement is currently in progress.

  2. Steroidogenic enzyme histochemistry in the testis of Sprague Dawley rats following the administration the water extracts from Carica papaya seed.

    Science.gov (United States)

    Uche-Nwachi, E O; Mitchell, C V; McEwen, C

    2011-01-01

    Water extracts from pawpaw seed have been reported to reversibly decrease the testicular weight and to suppress spermatogenesis, and fertility of Wistar rats. The reversible changes become evident, 30 - 45 days after the withdrawal of the extract. The possible effect of this extract on the activities of steroidogenic enzymes of the testis has not been investigated. Water extract of papaya seeds was administered to male Sprague Dawley rats ad libitum for 84 days. Following the discontinuation of the extracts, ten rats each were sacrificed on days 0, 10, 20 and 30 after the withdrawal. Their testes were quickly dissected out and frozen. Cryostat sections, 10µm thick were cut. These sections were used for immunohistochemical stains for side chain cleavage enzyme and aromatase, and for histochemical stains for 17-β Hydroxysteroid dehydrogenase, 3-β Hydroxysteroid dehydrogenase. We conclude that the water extract of papaya seed suppresses the activities of steroidogenic enzymes in the testis of Sprague Dawley rats, and that this may contribute to reversible suppression of spermatogenesis, a property that gives a possible male contraceptive potential.

  3. Modelos de demanda por manzanas frescas chilenas en países de la Unión Europea Equações de demanda para maçãs frescas, chilenas, em países da União Européia

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    Germán Lobos Andrade

    2005-04-01

    Full Text Available Se usó la técnica de regresión de mínimos cuadrados ordinarios para estimar la demanda por importaciones de manzanas frescas exportadas por Chile en cinco países de la Unión Europea. Para las estimaciones se utilizaron observaciones anuales del periodo 1984 a 2003. Los resultados demuestran que (1 la demanda por importaciones de manzanas frescas chilenas depende de las tradicionales variables ingreso o gasto en consumo per cápita, (2 los precios relativos y la variación del tipo de cambio real juegan un papel destacado en la explicación de la demanda por importaciones, y (3 la demanda responde de manera diferente frente a los cambios en las variables explicativas en cada mercado.Foi usada a técnica de regressão de mínimos quadrados ordinários para estimar a demanda das importações de maçãs frescas exportadas pelo cinco países da União Européia. Para fazer as estimações foram utilizadas observações anuais do período 1984 a 2003. Os resultados demostram que (1 a demanda por importações de maçãs frescas chilenas depende das tradicionais variáveis ingresso ou gasto em consumo per cápita, (2 os preços relativos e a variação do tipo de câmbio real tem una importante participação na explicação da demanda por importações, e (3 a demanda responde de forma diferente com relação às mudanças nas variáveis explicativas em cada mercado.

  4. Inhibition of the host proteasome facilitates papaya ringspot virus accumulation and proteosomal catalytic activity is modulated by viral factor HcPro.

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    Nandita Sahana

    Full Text Available The ubiquitin/26S proteasome system plays an essential role not only in maintaining protein turnover, but also in regulating many other plant responses, including plant-pathogen interactions. Previous studies highlighted different roles of the 20S proteasome in plant defense during virus infection, either indirectly through viral suppressor-mediated degradation of Argonaute proteins, affecting the RNA interference pathway, or directly through modulation of the proteolytic and RNase activity of the 20S proteasome, a component of the 20S proteasome, by viral proteins, affecting the levels of viral proteins and RNAs. Here we show that MG132, a cell permeable proteasomal inhibitor, caused an increase in papaya ringspot virus (PRSV accumulation in its natural host papaya (Carica papaya. We also show that the PRSV HcPro interacts with the papaya homologue of the Arabidopsis PAA (α1 subunit of the 20S proteasome, but not with the papaya homologue of Arabidopsis PAE (α5 subunit of the 20S proteasome, associated with the RNase activity, although the two 20S proteasome subunits interacted with each other. Mutated forms of PRSV HcPro showed that the conserved KITC54 motif in the N-terminal domain of HcPro was necessary for its binding to PAA. Co-agroinfiltration assays demonstrated that HcPro expression mimicked the action of MG132, and facilitated the accumulation of bothtotal ubiquitinated proteins and viral/non-viral exogenous RNA in Nicotiana benthamiana leaves. These effects were not observed by using an HcPro mutant (KITS54, which impaired the HcPro - PAA interaction. Thus, the PRSV HcPro interacts with a proteasomal subunit, inhibiting the action of the 20S proteasome, suggesting that HcPro might be crucial for modulating its catalytic activities in support of virus accumulation.

  5. Rapid Construction of Stable Infectious Full-Length cDNA Clone of Papaya Leaf Distortion Mosaic Virus Using In-Fusion Cloning.

    Science.gov (United States)

    Tuo, Decai; Shen, Wentao; Yan, Pu; Li, Xiaoying; Zhou, Peng

    2015-12-01

    Papaya leaf distortion mosaic virus (PLDMV) is becoming a threat to papaya and transgenic papaya resistant to the related pathogen, papaya ringspot virus (PRSV). The generation of infectious viral clones is an essential step for reverse-genetics studies of viral gene function and cross-protection. In this study, a sequence- and ligation-independent cloning system, the In-Fusion(®) Cloning Kit (Clontech, Mountain View, CA, USA), was used to construct intron-less or intron-containing full-length cDNA clones of the isolate PLDMV-DF, with the simultaneous scarless assembly of multiple viral and intron fragments into a plasmid vector in a single reaction. The intron-containing full-length cDNA clone of PLDMV-DF was stably propagated in Escherichia coli. In vitro intron-containing transcripts were processed and spliced into biologically active intron-less transcripts following mechanical inoculation and then initiated systemic infections in Carica papaya L. seedlings, which developed similar symptoms to those caused by the wild-type virus. However, no infectivity was detected when the plants were inoculated with RNA transcripts from the intron-less construct because the instability of the viral cDNA clone in bacterial cells caused a non-sense or deletion mutation of the genomic sequence of PLDMV-DF. To our knowledge, this is the first report of the construction of an infectious full-length cDNA clone of PLDMV and the splicing of intron-containing transcripts following mechanical inoculation. In-Fusion cloning shortens the construction time from months to days. Therefore, it is a faster, more flexible, and more efficient method than the traditional multistep restriction enzyme-mediated subcloning procedure.

  6. Rapid Construction of Stable Infectious Full-Length cDNA Clone of Papaya Leaf Distortion Mosaic Virus Using In-Fusion Cloning

    Directory of Open Access Journals (Sweden)

    Decai Tuo

    2015-12-01

    Full Text Available Papaya leaf distortion mosaic virus (PLDMV is becoming a threat to papaya and transgenic papaya resistant to the related pathogen, papaya ringspot virus (PRSV. The generation of infectious viral clones is an essential step for reverse-genetics studies of viral gene function and cross-protection. In this study, a sequence- and ligation-independent cloning system, the In-Fusion® Cloning Kit (Clontech, Mountain View, CA, USA, was used to construct intron-less or intron-containing full-length cDNA clones of the isolate PLDMV-DF, with the simultaneous scarless assembly of multiple viral and intron fragments into a plasmid vector in a single reaction. The intron-containing full-length cDNA clone of PLDMV-DF was stably propagated in Escherichia coli. In vitro intron-containing transcripts were processed and spliced into biologically active intron-less transcripts following mechanical inoculation and then initiated systemic infections in Carica papaya L. seedlings, which developed similar symptoms to those caused by the wild-type virus. However, no infectivity was detected when the plants were inoculated with RNA transcripts from the intron-less construct because the instability of the viral cDNA clone in bacterial cells caused a non-sense or deletion mutation of the genomic sequence of PLDMV-DF. To our knowledge, this is the first report of the construction of an infectious full-length cDNA clone of PLDMV and the splicing of intron-containing transcripts following mechanical inoculation. In-Fusion cloning shortens the construction time from months to days. Therefore, it is a faster, more flexible, and more efficient method than the traditional multistep restriction enzyme-mediated subcloning procedure.

  7. Effects of intelligent modified atmosphere packaging on the quality of Carica papaya%活性气调包装对番木瓜品质的影响

    Institute of Scientific and Technical Information of China (English)

    侯美玲; 段华伟; 胡长鹰

    2011-01-01

    研究了内含CO:吸附剂的活性气调包装时番木瓜综合品质的影响.与未加吸附剂的普通气调相比,活性气调包装能够降低番木瓜的氧气消耗率,推迟番木瓜果肉内多糖和还原糖的达峰时间,减少番木瓜的失水率,提高气调包装对番木瓜的保鲜效果,但对维生素C影响不显著.研究结果表明活性气调包装在番木瓜保鲜贮藏中具有一定的推广应用价值.%The effects of active modified atmosphere packaging (MAP) with in-pack CO2 adsorbent upon papaya' s attributes were examined. Compared to routine MAP, the active MAP was able to depress the rate of oxygen consumption by papaya, delay the summit occurrence of sugar contents in pulp, reduce the weight loss of the fruit, and enhance the efficiency of MAP in the quality keeping of papaya. However,contents of vitamin C in papaya packaged by active MAP were observed changing in a similar way to that in MAP without CO2 adsorbent. These results indicated that the active MAP was of certain practical values in the postharvest storage of papaya fruit.

  8. Poder, Ideología y el Consenso de Washington: Desarrollo y Expansión de la Política Chilena de Vivienda.

    Directory of Open Access Journals (Sweden)

    Alan Gilbert

    2003-01-01

    Full Text Available Es bien sabido que a menudo la realidad supera la ficción. Esto ciertamente se aplica en el caso del modelo de subsidio habitacional en Chile. Desarrollado por los chilenos con ayuda de la ideología neo-liberal inventada en Chicago, las poderosas instituciones de Washington DC aparecen como actores menores en la escena chilena. Inicialmente, Chile necesitaba el apoyo y financiamiento del Banco Mundial pero una vez conseguidos, Chile siguió su propia agenda. El Banco de Desarrollo Interamericano y USAID no lograron mucho más. Chile era el amo y señor en su propia casa. ¿Fue ésta una victoria para la autonomía nacional por sobre el poder del financiamiento internacional?. Ciertamente no, porque el nuevo modelo que se estaba aplicando en Chile fue el precursor de mucho de lo que se estaba implementando desde Washington en los días de ajuste estructural. Chile “ganó” porque aceptó las reglas del nuevo juego establecido por instituciones muchísimo más poderosas que los bancos de desarrollo multilaterales. Washington del Desarrollo aprendió mucho de Chile y luego aplicó lo aprendido a los países más endeudados, más pequeños y menos sofisticados.

  9. Evolución de la industria chilena de fondos mutuos: análisis de su performance (2000-2005

    Directory of Open Access Journals (Sweden)

    Benito Umaña Hermosilla

    2013-01-01

    Full Text Available En el presente estudio, los autores desarrollan un análisis riguroso de la evolución de la Industria Chilena de Fondos Mutuos. Se aplican para ello las medidas tradicionales de performance, correspondientes a los autores Sharpe, Treynor y Jensen, poniendo para ello de manifiesto, que su funcionamiento correcto depende de una serie de circunstancias, que no siempre se cumplen en los mercados financieros. En estos casos se aplican medidas alternativas de medición de la performance, las cuales eliminan las incoherencias detectadas. Estas medidas fueron desarrolladas por los profesores Ferruz y Sarto (1997. El presente estudio comprende el periodo 2000- 2005. Se analizan rentabilidades mensuales correspondientes a 196 fondos mutuos de renta variable. Los índices alternativos no hicieron más que ratificar el resultado de los índices originales. Se concluye además que las gestoras con mayor patrimonio administrado no poseen los mejores índices de performance, similar situación ocurre con aquellas gestoras pertenecientes a la industria bancaria.

  10. Evolución de la industria chilena de fondos mutuos. Análisis de su performance (2000-2005

    Directory of Open Access Journals (Sweden)

    Benito Umaña Hermosilla

    2013-05-01

    Full Text Available En el presente estudio, los autores desarrollan un análisis riguroso de la evolución de la Industria Chilena de Fondos Mutuos. Se aplican para ello las medidas tradicionales de performance,  correspondientes a los autores Sharpe, Treynor y Jensen, poniendo para ello de manifiesto, que su funcionamiento correcto depende de una serie de circunstancias, que no siempre se cumplen en los mercados financieros. En estos casos se aplican  medidas alternativas de medición de la performance, las cuales eliminan las incoherencias detectadas. Estas medidas fueron desarrolladas por los profesores Ferruz y Sarto (1997. El presente estudio comprende el periodo 2000- 2005. Se analizan rentabilidades mensuales correspondientes a 196 fondos mutuos de renta variable. Los índices alternativos no hicieron más que ratificar el resultado de los índices originales. Se concluye además que las gestoras con mayor patrimonio administrado no poseen los mejores índices de performance, similar  situación ocurre con aquellas gestoras pertenecientes a la industria bancaria.

  11. Agua, Poder y Discursos: Conflictos Socio-territoriales por la construcción de centrales hidroeléctricas en la Patagonia Chilena

    Directory of Open Access Journals (Sweden)

    Romero Toledo, Hugo

    2009-12-01

    Full Text Available Chile is going to celebrate soon two hundred years of republican life and thirty five years of the application of a neoliberal economic model, in respect of which there are a lot of conflicts related with the land/capital dicotomy and water like a main resource. One of these conflicts is currently taking place in Chilean Patagonia, where, as a direct result of water privatization, a large hydropower project, supported by transnational companies collisions with traditional production and highly environmental quality landscapes.

    Chile está próximo a celebrar su Bicentenario de vida republicana y sus treinta y cinco años desde la aplicación de un estricto modelo económico neoliberal, donde han surgido numerosos conflictos que involucran a la dicotomía capital/territorio y que implican al agua como recurso principal. Uno de estos conflictos es el que actualmente ocurre en la Región de Aysén en la Patagonia Chilena donde, producto de la privatización del agua, un megaproyecto de producción hidroeléctrica de inversión transnacional colisiona con territorios de alta calidad ambiental y producción tradicional.

  12. CONOCIMIENTO DE LOS ESTUDIANTES DE MEDICINA DE LA UNIVERSIDAD DE CONCEPCIÓN SOBRE LA LEY CHILENA DE DERECHOS Y DEBERES DE LOS PACIENTES

    Directory of Open Access Journals (Sweden)

    Ricardo Varas Abarca

    2014-01-01

    Full Text Available Objetivo: Caracterizar el nivel de conocimiento de la ley en los estudiantes de Medicina de la Universidad de Concepción. Metodología: Estudio descriptivo de corte transversal, realizado a través de una encuesta por plataforma en internet a estudiantes voluntarios de la carrera de Medicina de la Universidad de Concepción. Se contó con la participación de 133 estudiantes, cuyos datos entregados fueron procesados por el programa Microsoft Excel. Resultados: De 103 encuestas válidas, 55,34% fueron aplicadas a hombres y el 80,58% de los estudiantes sabía de la existencia de la ley. De estos, el 80,72% dijo conocer algún derecho y el 67,47% calificó con un conocimiento adecuado. El 70,52% consideró que esta ley es útil y a un 88.35% le gustaría saber más de ella. Conclusiones: En conclusión, un 67,47% de los estudiantes de Medicina de la Universidad de Concepción tiene un conocimiento adecuado sobre la Ley de los Derechos y Deberes de los Pacientes, lo cual es un aliento para continuar trabajando en el desarrollo de la Bioética en las universidades chilenas. Palabras Clave: Bioética, pacientes, derechos del paciente, estudiantes de medicina.

  13. La antropología rural chilena en las dos últimas décadas: situación y perspectivas

    Directory of Open Access Journals (Sweden)

    Roberto Hernández

    2009-05-01

    Full Text Available El presente trabajo proporciona una revisión analítica y proyectiva del desarrollo de la antropología rural en Chile durante las dos últimas décadas. La investigación se ha basado en un análisis de los estudios de antropología rural chilena realizados durante los últimos veinte años, centrados especialmente sobre los cambios ocurridos en la sociedad rural. Se identificó un tratamiento particular y descriptivo de los fenómenos rurales, pero sólo algunos intentos de producir elementos teórico-conceptuales que permitan comprender e interpretar los procesos socioculturales que se han producido en el sector rural chileno. Como consecuencia de esta situación, los autores ofrecen algunas propuestas para la próxima década, con el fin de ofrecer un desarrollo disciplinario consecuente con los retos impuestos por la nueva ruralidad.

  14. Chistes par(ra reordenar el canon: Roberto Bolaño, Nicanor Parra y la poesía chilena

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    Benjamin Loy

    2014-12-01

    Full Text Available “Todo se lo debo a Parra” – con estas y semejantes palabras de elogio Roberto Bolaño le asignó un lugar central al antipoeta chileno dentro de su amplio canon personal. No obstante, la amplia crítica bolañiana hasta el momento no pareciera haber estudiado a fondo las múltiples dimensiones de esta relación entre los dos autores chilenos. El presente estudio pretende analizar, por lo tanto, de qué manera los postulados ético-estéticos de Parra y de su antipoesía le sirven a Bolaño como punto de referencia clave para reordenar el canon de la literatura chilena contemporánea. Además, se discutirá el papel clave que desempeña Bolaño en el renovado interés de la crítica en la obra de Nicanor Parra durante los últimos años.

  15. Contra-tradición: prácticas críticas y desestabilizadoras de escritoras chilenas de principios de siglo XX

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    Marina d. A. Alvarado Cornejo

    2009-01-01

    Full Text Available This article reviews the writing of critical speeches of the Chilean poets and storytellers Gabriela Mistral (1889-1957, Teresa Wilms Montt (1893-1921, Maria Monvel (1899-1936, Maria Luisa Bombal (1910-1980 and Maria Carolina Geel (1913-1996, who appropriated this genre as a strategy to reassert networks between women and, at the same time, be inserted inside the complex chilean and latinamerican cultural and literary field. Este artículo revisa la escritura de discursos críticos de las poetas y narradoras chilenas Gabriela Mistral (1889-1957, Teresa Wilms Montt (1893- 921, María Monvel (1899-1936, María Luisa Bombal (1910-1980 y María Carolina Geel (1913-1996, quienes se apropian de este género a modo de estrategia para reafirmar las redes entre mujeres y, a su vez, insertarse dentro del complejo campo cultural y literario chileno y latinoamericano.

  16. Respuesta del microfitoplancton a la adición de nitrato y ácido silícico en fiordos de la Patagonia chilena

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    Pamela Labbé-Ibáñez

    2015-03-01

    Full Text Available La Patagonia chilena está experimentando un aumento del uso del suelo y océano costero y el acelerado derretimiento de algunos glaciares, por lo que uno de los escenarios a futuro es que los fiordos adyacentes reciban cantidades variables de agua dulce y nutrientes inorgánicos. Se estudió la respuesta de ensambles de fitoplancton de los fiordos Reloncaví en invierno y Comau en primavera, a la adición de nitrato y ácido silícico en experimentos de microcosmos. Los ensambles fitoplanctónicos estudiados respondieron a la adición de nutrientes con incrementos en la abundancia celular biomasa autotrófica (clorofila-a y cambios en la composición específica, destacando el cambio de dominancia de especies de diatomeas pennadas a céntricas formadoras de cadenas. Estos resultados dan indicios acerca de los factores que afectan a las comunidades microalgales en un escenario de cambio climático e intervención antrópica de los fiordos de la Patagonia Norte.

  17. La oposición a la dictadura de Miguel Primo de Rivera a través de la prensa mercurial chilena (1923-1930

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    Mario Valdés Urrutia

    2011-06-01

    Full Text Available Este artículo examina algunos aspectos del accionar opositor al gobierno de Miguel Primo de Rivera desde una mirada sudamericana: el diario El Mercurio, influyente periódico en la sociedad chilena. Las reacciones frente al alzamiento primorriverista de 1923; la crítica política junto a algunos intentos de defenestrar la Dictadura;  y el  rechazo al proyecto de institucionalización autoritaria y antiliberal, son los temas abordados.Palabras claves: Primo de Rivera – Dictadura – Oposición – Visión de El Mercurio.___________________________Abstract:This article examines certain aspects of the opposition to the dictatorshipof Miguel Primo de Rivera from a South American point of view: that of El  Mercurio, an influential Chilean newspaper. The reaction to the coup d'etat of Primo de Rivera in 1923, political criticism together with certain attempts to unseat the dictator, and the rejection of the authoritarian and antiliberal political project are all considered. KeyWords: Primo de Rivera, Dictatorship, Opposition, Viewpoint of El Mercurio

  18. Un amor estratificado: narrativas, prácticas y la infraestructura del amor de tres mujeres chilenas de diferentes estratos sociales

    Directory of Open Access Journals (Sweden)

    Amanda Rutllant

    2013-08-01

    Full Text Available El presente artículo es un estudio exploratorio que busca incorporar la variable estratificación a la actual discusión académica en torno a lastransformaciones en la intimidad y lasformas de vivir el amor en la modernidad tardía producto de los proceso de individuación. Asimismo se hace hincapié en la conceptualización material del amor, considerando las prácticas y objetos de consumo amoroso como factores determinantes de la vivencia del amor. A través de un estudio exploratorio/comparativo basado en el análisis de historias de vida de tres mujeres chilenas de diferentes estratossocio-económicos se busca problematizar las categorías conceptuales que hoy se utilizan en el debate sociológico en torno al amor. Como se verá, el factor estratificación demuestra que el amor se vive de formas diferentes dependiendo del estrato social así como también de las materialidades.

  19. La política habitacional chilena: Alternativas de acceso a la vivienda para las familias más pobres.

    Directory of Open Access Journals (Sweden)

    Jaime Ravinet de la Fuente

    2004-01-01

    Full Text Available El presente artículo contiene las principales razones que llevaron al Ministerio de Vivienda y Urbanismo a introducir, en el año 2001, modificaciones a la Política Habitacional, y se exponen los objetivos y los programas de la Nueva Política Habitacional Chilena, que fueron producto de dichos cambios, generando una variedad de alternativas que en la actualidad permite el acceso a la vivienda propia a las diversas familias del país, en particular para aquellos grupos de menores recursos y con mayores dificultades para resolver su problema habitacional. Asimismo, se indican algunos logros de dicha Política en lo que va corrido desde su entrada en vigencia, particularmente en lo que dice relación con el Fondo Concursable para Proyectos Habitacionales Solidarios, como lo son la focalización, inversión, cobertura y beneficiados. Por último, se señalan los desafíos para el Ministerio para el año 2004.

  20. Psicopatía y delincuencia: comparaciones y diferencias entre ofensores sexuales y delincuentes comunes en una cárcel chilena

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    José Cabrera Sánchez

    2014-05-01

    Full Text Available En esta investigación se compararon ofensores sexuales y delincuentes comunes de una cárcel chilena en relación con la psicopatía. La muestra constó de 57 agresores sexuales y 82 delincuentes comunes, todos condenados y recluidos en el Centro Cumplimiento Penitenciario de la ciudad de Puerto Montt, Chile. El instrumento utilizado en esta investigación fue el Psychopathy Checklist-Revised (PCL-R, de Robert Hare. Los resultados demuestran que el nivel de psicopatía parece no influir de manera significativa en el hecho de estar condenado por algún tipo de delito sexual; por otra parte, se aprecian niveles moderados de psicopatía en personas condenadas por delitos no violentos. A medida que aumentan las características psicopáticas se aprecia una asociación positiva con la participación en delitos violentos y/o de sangre. Se concluye que los ofensores sexuales y delincuentes comunes reincidentes presentan niveles mayores de psicopatía.

  1. Adsorption of saponin compound in Carica papaya leaves extract using weakly basic ion exchanger resin

    Science.gov (United States)

    Abidin, Noraziani Zainal; Janam, Anathasia; Zubairi, Saiful Irwan

    2016-11-01

    Adsorption of saponin compound in papaya leaves juice extract using Amberlite® IRA-67 resin was not reported in previous studies. In this research, Amberlite® IRA-67 was used to determine the amount of saponin that can be adsorbed using different weights of dry resin (0.1 g and 0.5 g). Peleg model was used to determine the maximum yield of saponin (43.67 mg) and the exhaustive time (5.7 days) prior to a preliminary resin-saponin adsorption study. After adsorption process, there was no significant difference (p>0.05) in total saponin content (mg) for sample treated with 0.1 g (3.79 ± 0.55 mg) and sample treated with 0.5 g (3.43 ± 0.51 mg) dry weight resin. Long-term kinetic adsorption of resin-saponin method (>24 hours) should be conducted to obtain optimum freed saponin extract. Besides that, sample treated with 0.1 g dry weight resin had high free radical scavenging value of 50.33 ± 2.74% compared to sample treated with 0.5 g dry weight resin that had low free radical scavenging value of 24.54 ± 1.66% dry weights. Total saponin content (mg), total phenolic content (mg GAE) and free radical scavenging activity (%) was investigated to determine the interaction of those compounds with Amberlite® IRA-67. The RP-HPLC analysis using ursolic acid as standard at 203 nm showed no peak even though ursolic acid was one of the saponin components that was ubiquitous in plant kingdom. The absence of peak was due to weak solubility of ursolic acid in water and since it was only soluble in solvent with moderate polarity. The Pearson's correlation coefficient for total saponin content (mg) versus total phenolic content (mg GAE) and radical scavenging activity (%) were +0.959 and +0.807. Positive values showed that whenever there was an increase in saponin content (mg), the phenolic content (mg GAE) and radical scavenging activity (%) would also increase. However, as the resin-saponin adsorption was carried out, there was a significant decrease of radical scavenging activity

  2. Pseudomonas syringae pv. tabaci in papaya seedlings Pseudomonas syringae pv. tabaci em plântulas de mamoeiro

    Directory of Open Access Journals (Sweden)

    Luís Otávio S. Beriam

    2006-03-01

    Full Text Available The natural occurrence of Pseudomonas syringae pv. tabaci causing leaf spot symptoms in papaya seedlings is reported. The pathogen was identified through biochemical, physiological, serological, and molecular assays and artificial inoculations in papaya plants. It was also shown that the strains were pathogenic to bean and tobacco plants. The restriction patterns obtained with Afa I, Alu I, Dde I, Hae III, Hpa II, Hinf I, Sau 3A I and Taq I of the PCR-RFLP of 16S-23S DNAr were identical to the P. s. pv. tabaci patterns. Primers corresponding to hrpL gene of P. syringae were also tested and the results grouped the papaya strains with P s. pv. tabaci. Bacterial strains were deposited at Coleção de Culturas IBSBF, Instituto Biológico, Campinas, Brazil, under access numbers 1687 and 1822.É relatada a ocorrência natural de Pseudomonas syringae pv. tabaci causando sintomas de lesões foliares em plântulas de mamoeiro. O patógeno foi identificado por meio de testes bioquímicos, fisiológicos, serológicos e moleculares, além de ensaios de patogenicidade em plantas de mamoeiro, feijoeiro e fumo. Os padrões de restrição obtidos com as enzimas Afa I, Alu I, Dde I, Hae III, Hpa II, Hinf I, Sal 3A I e Taq I, utilizando-se a técnica de PCR-RLFP da região espaçadora 16S-23S do DNA ribossômico, foram idênticos àqueles apresentados para P. s. pv. tabaci. Primers correspondentes ao gene hrpL de P. syringae foram também testados e os resultados obtidos permitiram agrupar as linhagens isoladas de mamão com P. s. pv. tabaci. Linhagens bacterianas estão depositadas na coleção de culturas IBSBF, Instituto Biológico, Campinas, sob n. 1687 e 1822.

  3. Bionomics of the apefly, Spalgis epius (Lepidoptera: Lycaenidae, predatory on the papaya mealybug, Paracoccus marginatus (Hemiptera: Pseudococcidae, in Thailand

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    Intawat Burikam

    2012-02-01

    Full Text Available We investigated the life history, growth ratio and feeding potential of the hemipterophagous butterfly or the apefly,Spalgis epius (Westwood (Lepidoptera: Lycaenidae: Miletinae, a promising augmentative biological control agent of thepapaya mealybug, Paracoccus marginatus Williams & Granara de Willink (Hemiptera: Pseudococcidae, which is an invasivealien species detected in 2008 in Thailand. The investigation was carried out under laboratory conditions at 25-30ºC and 40-60% RH using papaya mealybug reared on Thai pumpkin as its prey. The mean duration of the egg, larval and pupal stageswere 3.54±0.50, 12.01±2.40, and 10.32±0.88 days, respectively, and the mean duration from egg to adult emergence was 25.87±3.78 days. The geometric growth ratio of successive larval instars using the head capsule width as a parameter was 1.65 andconformed to Dyar’s Law. As for the predatory potential of S. epius, the total number of papaya mealybug consumed duringthe larval stage were 4,115.75±553.28 eggs, 281.25±45.08 nymphs and 77.50±16.52 female adults. The instar-specific preyconsumption from the first to fifth larval instars in the order of eggs, nymphs and female adults were 170.00±26.32, 4.25±1.71and 2.00±0.82; 654.00±67.97, 35.35±12.48 and 10.25±4.29; 1,376.75±130.95, 93.25±11.70 and 23.50±3.94; 1,426.50±252.93,110.75±15.60 and 31.00±5.25; and 31.00±5.25, 37.75±3.59 and 10.75±2.22, respectively. These findings warrant furtherinvestigation into the amenability and suitability of the entomophagous apefly, S. epius, for as an integrated pest managementstrategy for the augmentative biological control of the papaya mealybug in Thailand.

  4. Métodos de extracción de semilla en papaya Golden y la relación con la longevidad Extraction methods in Golden type papaya seed and relationship with longevity

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    Julio César García Dean

    2011-04-01

    Full Text Available Es muy escasa la información disponible, relacionada al manejo de poscosecha de semilla de papaya (Carica papaya L. y su influencia en la calidad fisiológica de la semilla, sobre todo en germinación y vigor. El trabajo de investigación se realizó en el laboratorio de producción de semillas del Centro de Capacitación y Desarrollo de Tecnología de Semillas, en el invernadero número uno de la Universidad Autónoma Agraria "Antonio Narro" (UAAAN durante 2008. La finalidad del trabajo fue evaluar seis tratamientos de extracción de semilla: se utilizó ácido clorhídrico (HCl a 0.3 y 0.5% durante una hora de reposo, ácido sulfúrico (H2SO4 a 0.3% a 1.5 h de reposo, hipoclorito de sodio (NaClO al 5% con media hora de reposo y fermentación a 48 y 72 h. La semilla extraída fue almacenada durante 90, 180 y 240 días a 4 °C. Se evaluó la germinación y vigor de la semilla. Se utilizó un diseño completamente al azar con arreglo factorial. Los resultados indicaron que la calidad fisiológica de la semilla, fue afectada por los efectos de deterioro que causa el almacenamiento y por los métodos de extracción de semilla; sin embargo, la semilla conserva su calidad fisiológica hasta los 240 días de almacenamiento, en los tratamientos a base de HCl al 0.3% y fermentación por 24 h con 93.3 y 91.2% de germinación después de 240 días de almacenamiento.It is very scarce available information related to post-harvest handling of papaya seed (Carica papaya L. and their influence in seed physiologic quality, mainly in germination and vigor. The investigation work was carried out in seeds production laboratory of Center of Training and Development of Seeds Technology, in greenhouse number one of Universidad Autónoma Agraria "Antonio Narro" (UAAAN during 2008. Aim of work was to evaluate six treatments of seed extraction: hydrochloric acid was used (HCl at 0.3 and 0.5% during one hour of rest, sulfuric acid (H2SO4 at 0.3% with 1.5 h of rest

  5. Sitios web internacionales como herramientas de internacionalización: un análisis de las universidades chilenas y españolas/International web sites as an internationalization tools: an analysis of Chilean and Spanish Universities

    Directory of Open Access Journals (Sweden)

    José Luis Meza-Orellana

    2015-05-01

    Full Text Available El presente estudio tiene como objetivo identificar y describir las conductas de comunicación dirigidas a stakeholders internacionales, desarrolladas por las universidades chilenas y españolas en la web. Mediante el método de análisis de contenido, indagamos cómo, el 87% del total de universidades existentes en ambos países, 49 chilenas y 73 españolas, utilizan soportes web para su comunicación internacional. El trabajo midió variables como la presencia de sitios web específicos para la internacionalización, la oferta de contenido en otros idiomas, el acceso a redes sociales y los tipos de contenidos más frecuentemente publicados en los sitios específicos para stakeholders internacionales y comparó los desempeños entre ambos países. Los resultados muestran que existe una relación directa entre el número de estudiantes extranjeros matriculados en la universidad y el uso de sitios web específicos, las universidades en su mayoría usa el idioma inglés y muy pocas ofrecen acceso a redes sociales específicas para internacionalización. Las universidades españolas mostraron mejores desempeños que las chilenas. Los resultados podrían aportar luces respecto de la gestión de este tipo de herramientas para la internacionalización y particularmente para la atracción de estudiantes extranjeros.

  6. RAP2.4a Is Transported through the Phloem to Regulate Cold and Heat Tolerance in Papaya Tree (Carica papaya cv. Maradol): Implications for Protection Against Abiotic Stress

    Science.gov (United States)

    Arroyo-Herrera, Ana; Rodriguez-Corona, Ulises; Sanchez-Teyer, Felipe; Espadas-Alcocer, Jorge; Espadas-Gil, Francisco; Barredo-Pool, Felipe; Castaño, Enrique; Rodriguez-Zapata, Luis Carlos

    2016-01-01

    Plants respond to stress through metabolic and morphological changes that increase their ability to survive and grow. To this end, several transcription factor families are responsible for transmitting the signals that are required for these changes. Here, we studied the transcription factor superfamily AP2/ERF, particularly, RAP2.4 from Carica papaya cv. Maradol. We isolated four genes (CpRap2.4a, CpRAap2.4b, CpRap2.1 and CpRap2.10), and an in silico analysis showed that the four genes encode proteins that contain a conserved APETALA2 (AP2) domain located within group I and II transcription factors of the AP2/ERF superfamily. Semiquantitative PCR experiments indicated that each CpRap2 gene is differentially expressed under stress conditions, such as extreme temperatures. Moreover, genetic transformants of tobacco plants overexpressing CpRap2.4a and CpRap2.4b genes show a high level of tolerance to cold and heat stress compared to non-transformed plants. Confocal microscopy analysis of tobacco transgenic plants showed that CpRAP2.4a and CpRAP2.4b proteins were mainly localized to the nuclei of cells from the leaves and roots and also in the sieve elements. Moreover, the movement of CpRap2.4a RNA in tobacco grafting was analyzed. Our results indicate that CpRap2.4a and CpRap2.4b RNA in the papaya tree have a functional role in the response to stress conditions such as exposure to extreme temperatures via direct translation outside the parental RNA cell. PMID:27764197

  7. Modelagem empírica do processo de biodegradação de efluentes protéicos por enzimas de Carica papaya sp. Empirical modeling of biodegradation process of proteic effluents by enzymes of Carica papaya sp.

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    Joana P. M. Biazus

    2006-06-01

    Full Text Available O objetivo deste trabalho foi modelar empiricamente a biodegradação de efluentes protéicos por enzimas papaínas do látex de Carica papaya sp. num biorreator agitado, para facilitar a otimização do processo por metodologia de análise de superfície de resposta (RSM e aplicar em tratamento de efluentes provenientes de indústrias de processamento de carne. As análises das curvas de biodegradação mostraram que o tempo de residência para os biorreatores está entre 28 e 30 h. O modelo empírico que mais se ajustou aos dados de biodegradação sob influência dos fatores (pH e temperatura foi o de um hiperplano e a região de operação otimizada para a degradação de proteínas pelas papaínas foi aquela que apresentou os maiores valores de temperatura e menores valores de pH.The objective of this research was to model empirically the proteic effluent biodegradation by papains enzymes from Carica papaya sp. in an agitated bioreactor, to facilitate the optimization process by response surface methodology (RSM and to apply in treatment of effluents from meat processing industries. The analysis of biodegradation curves showed that bioreactors residence time is between 28 and 30 h. Hiper plane empirical model was the one that best fitted to biodegradation data under the influence factors (pH and temperature and the optimized operation zone for the protein biodegradation by papains was the one that presented the high temperature and low pH values.

  8. Estrés Materno y Configuración Familiar: Estudio comparativo en Familias Chilenas Monoparentales y Nucleares de bajos ingresos / Maternal Stress and Family Constitution: Comparative Study on Chilean, Single-Mother and Nuclear, Low-Income Families

    OpenAIRE

    Marcia Olhaberry; Chamarrita Farkas

    2012-01-01

    Los estudios sobre estrés materno durante la crianza infantil han considerado variables contextuales para explicarlo. El nivel socioeconómico, así como la configuración familiar han sido variables relevantes, asociándose monoparentalidad en familias de bajos ingresos a mayores niveles de estrés materno. Se estudian los niveles de estrés materno en familias chilenas nucleares y monoparentales de N.S.E. bajo, considerando el estrés en distintas dimensiones, asociado al rol materno, a la interac...

  9. Metabolismo de carboidratos durante o amadurecimento do mamão (Carica papaya L. Cv. Solo: influência da radiação gama Influence of gamma radiation on carbohydrates metabolism of ripening papaya (Carica papaya L. cv. Solo

    Directory of Open Access Journals (Sweden)

    M. L. P. A. GOMEZ

    1999-05-01

    Full Text Available A irradiação de alimentos é um tratamento pós-colheita bastante promissor, capaz de atuar tanto na desinfestação como no aumento da vida útil de frutos. O mamão é um fruto com curta vida pós-colheita, levando de 6 a 12 dias para atingir a senescência. Além disto, a infestação por larvas de mosca diminui sensivelmente o seu potencial de exportação. O objetivo deste trabalho foi verificar a ação de uma dose de 0,5 kGy de irradiação gama, normalmente utilizada para desinfestação e aumento de vida útil dos frutos, sobre o comportamento dos carboidratos solúveis durante o amadurecimento do mamão (Carica papaya L. cv. Solo, e sobre as enzimas relacionadas ao metabolismo da sacarose: sacarose sintase (SS, sacarose-fosfato sintase (SPS e invertases ácida e neutra. Os resultados demonstraram que a irradiação não exerce efeito sobre o teor de glicose e frutose, ou sobre a respiração e atividade da SS. Entretanto, a composição dos açúcares solúveis totais e da sacarose, e a atividade da SPS e das invertases foram afetados.Food irradiation is one of the most promising treatments that can be utilized for fruits desinfestation and extension of shelf life. The authors studied the influence of 0,5 kGy of Gamma irradiation on the soluble carbohydrates composition of papaya (Carica papaya L. cv. Solo fruit, and on sucrose metabolizing enzymes: sucrose synthase (SS, sucrose-phosphate synthase (SPS, acid and neutral invertases activities, during ripening. The results demonstred that ethylene production, total soluble sugars, sucrose content, and sucrose-phosphate synthase and invertases activities were affected by irradiation, but not respiration, glucose and fructose content, and SS activity.

  10. Efeito do cultivo do mamoeiro (Carica papaya L. em ambiente protegido sobre a ocorrência de ácaros fitófagos e moscas-brancas Effect of papaya (Carica papaya L. cultivated in a protected environment on the occurrence of phytophagous mites and whiteflies

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    Marineide Rosa Vieira

    2004-12-01

    Full Text Available O efeito do cultivo do mamoeiro em ambiente protegido foi estudado em três condições: sem cobertura e em dois telados construídos com tela de propileno branca, com malhas de 2 x 2 mm e 2 x 1 mm. Nessa área foram feitas avaliações na cultivar Baixinho de Santa Amália, contando-se o número de plantas com sintomas de ataque recente, para o ácaro branco Polyphagotarsonemus latus, com sintomas e presença de ácaros, para o ácaro rajado Tetranychus urticae e com presença de adultos ou ninfas nas folhas, no caso das moscas-brancas Trialeurodes sp., Bemisia tabaci biótipo B e uma terceira espécie não identificada. Para moscas-brancas, também foram realizadas contagens de ninfas e exúvias em laboratório. O cultivo em ambiente protegido favoreceu a sobrevivência e o desenvolvimento populacional das espécies estudadas, sendo que algumas possíveis causas são discutidas no texto. Considerando-se que o cultivo protegido pode ser uma boa alternativa para o controle de viroses, como o mosaico do mamoeiro, problema limitante para a cultura, estratégias de manejo de pragas nesses ambientes devem ser desenvolvidas, para viabilizar o seu uso.It was measured the effect of cultivating papaya cultivar "Baixinho de Santa Amália" in a protected environment and in three situations: without shade, and under screen shades of white polyethylene mesh sizes 2 x 2 mm or 2 x 1 mm. It was registered the number of plants with symptoms of recent attacks of the broad mite (Polyphagotarsonemus latus, symptoms and presence of the two-spotted spider mite (Tetranychus urticae and the presence on leaves of adults or nymphs of whiteflies (Trialeurodes sp., Bemisia tabaci biotype B and a third unidentified species. Whitefly nymph and exuviae numbers were also counted in the laboratory. Cultivation in a protected environment favored the survival and development of the species under study, and some possible reasons for these are discussed along the text. Since

  11. Mesophilic anaerobic co-digestion of poultry dropping and Carica papaya peels: Modelling and process parameter optimization study.

    Science.gov (United States)

    Dahunsi, S O; Oranusi, S; Owolabi, J B; Efeovbokhan, V E

    2016-09-01

    The study evaluated anaerobic co-digestion of poultry dropping and pawpaw peels and the optimization of important process parameters. The physic-chemical analyses of the substrates were done using standard methods after application of mechanical, thermal and chemical pre-treatments methods. Gas chromatography analysis revealed the gas composition to be within the range of 66-68% methane and 18-23% carbon dioxide. The study equally revealed that combination of the different pre-treatment methods enhanced enormous biogas yield from the digestion. Optimization of the generated biogas data were carried out using the Response Surface Methodology and the Artificial Neural Networks. The coefficient of determination (R(2)) for RSM (0.9181) was lower compare to that of ANN (0.9828). This shows that ANN model gives higher accuracy than RSM model for the current. Further usage of Carica papaya peels for biogas generation is advocated.

  12. Nucleotide sequence of the capsid protein gene and 3' non-coding region of papaya mosaic virus RNA.

    Science.gov (United States)

    Abouhaidar, M G

    1988-01-01

    The nucleotide sequences of cDNA clones corresponding to the 3' OH end of papaya mosaic virus RNA have been determined. The 3'-terminal sequence obtained was 900 nucleotides in length, excluding the poly(A) tail, and contained an open reading frame capable of giving rise to a protein of 214 amino acid residues with an Mr of 22930. This protein was identified as the viral capsid protein. The 3' non-coding region of PMV genome RNA was about 121 nucleotides long [excluding the poly(A) tail] and homologous to the complementary sequence of the non-coding region at the 5' end of PMV RNA. A long open reading frame was also found in the predicted 5' end region of the negative strand.

  13. Inheritance of resistance to papaya ringspot virus-watermelon strain (PRSV-W in ‘Whitaker’ summer squash line

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    Cícero B Menezes

    2015-12-01

    Full Text Available The objective of this work was to study the genetic control of the PRSV-W (Papaya Ringspot Virus – Watermelon Strain resistance in Cucurbita pepo ‘Whitaker’ line. Plants of parental lines Whitaker (resistant and Caserta (susceptible, and of the generations F1, F2, BC11 and BC12 were evaluated for their reactions to PRSV-W. Caserta plants showed severe mosaic symptoms, while Whitaker grew vigorously and remained almost totally symptom-free. Most of the F1, F2and backcross plants also presented severe mosaic symptoms. Data were used to test a hypothesis of monogenic inheritance under different presumed degrees of dominance, and genetic models were tested using maximum likelihood tests of genetic control. Broad-sense heritability was of 0.57 for the first evaluation. Resistance to PRSV-W in C. pepo ‘Whitaker’ is due to a major gene effect summed to polygenic effects.

  14. 番木瓜人工杂交制种技术%Artificial hybridization technique for papaya seed production

    Institute of Scientific and Technical Information of China (English)

    孙德权; 陆新华; 吕玲玲

    2007-01-01

    番木瓜(Carica papaya L.)为番木瓜科(Caricaceae)番木瓜属(Carica)多年生草本植物,属热带、南亚热带常绿性果树。番木瓜株性较复杂,存在雄株、雌株和两性株.传统上采用种子进行繁殖。种问杂交是选育高产、优质、抗病品种的重要途径。根据近几年的试验研究,现就人工隔离杂交制种技术作一介绍。

  15. Green synthesis of colloidal copper oxide nanoparticles using Carica papaya and its application in photocatalytic dye degradation

    Science.gov (United States)

    Sankar, Renu; Manikandan, Perumal; Malarvizhi, Viswanathan; Fathima, Tajudeennasrin; Shivashangari, Kanchi Subramanian; Ravikumar, Vilwanathan

    2014-03-01

    Copper oxide (CuO) nanoparticles were synthesized by treating 5 mM cupric sulphate with Carica papaya leaves extract. The kinetics of the reaction was studied using UV-visible spectrophotometry. An intense surface Plasmon resonance between 250-300 nm in the UV-vis spectrum clearly reveals the formation of copper oxide nanoparticles. The results of scanning electron microscopy (SEM) and dynamic light scattering (DLS) exhibited that the green synthesized copper oxide nanoparticles are rod in shape and having a mean particle size of 140 nm, further negative zeta potential disclose its stability at -28.9 mV. The Fourier-transform infrared (FTIR) spectroscopy results examined the occurrence of bioactive functional groups required for the reduction of copper ions. X-ray diffraction (XRD) spectra confirmed the copper oxide nanoparticles crystalline nature. Furthermore, colloidal copper oxide nanoparticles effectively degrade the Coomassie brilliant blue R-250 dye beneath the sunlight.

  16. Green synthesis of colloidal copper oxide nanoparticles using Carica papaya and its application in photocatalytic dye degradation.

    Science.gov (United States)

    Sankar, Renu; Manikandan, Perumal; Malarvizhi, Viswanathan; Fathima, Tajudeennasrin; Shivashangari, Kanchi Subramanian; Ravikumar, Vilwanathan

    2014-01-01

    Copper oxide (CuO) nanoparticles were synthesized by treating 5 mM cupric sulphate with Carica papaya leaves extract. The kinetics of the reaction was studied using UV-visible spectrophotometry. An intense surface Plasmon resonance between 250-300 nm in the UV-vis spectrum clearly reveals the formation of copper oxide nanoparticles. The results of scanning electron microscopy (SEM) and dynamic light scattering (DLS) exhibited that the green synthesized copper oxide nanoparticles are rod in shape and having a mean particle size of 140 nm, further negative zeta potential disclose its stability at -28.9 mV. The Fourier-transform infrared (FTIR) spectroscopy results examined the occurrence of bioactive functional groups required for the reduction of copper ions. X-ray diffraction (XRD) spectra confirmed the copper oxide nanoparticles crystalline nature. Furthermore, colloidal copper oxide nanoparticles effectively degrade the Coomassie brilliant blue R-250 dye beneath the sunlight.

  17. Dependência micorrízica de variedades comerciais de mamoeiro Mycorrhizal dependency of papaya commercial varieties

    Directory of Open Access Journals (Sweden)

    Aldo Vilar Trindade

    2001-12-01

    Full Text Available O genótipo da planta é fator preponderante na resposta à micorriza. Avaliou-se, neste trabalho, a resposta a fósforo e o grau de dependência micorrízica de variedades de mamoeiro (Carica papaya L.. O experimento foi realizado em casa de vegetação da Embrapa-Centro Nacional de Pesquisa de Mandioca e Fruticultura, com quatro variedades, testando-se seis doses de P: 0, 20, 40, 80, 140, 280 mg dm-3 e três tratamentos fúngicos (um controle não-micorrizado e a inoculação de Glomus clarum ou Gigaspora margarita. Foram estimados os seguintes parâmetros: dose de P para máxima eficiência micorrízica; valor T'; benefício da aplicação do P; benefício micorrízico; eficiência simbiótica, e dependência micorrízica. Plantas não-micorrizadas apresentaram resposta quadrática ao P aplicado e as inoculadas seguiram modelo raiz-quadrático, com grande incremento de matéria seca da parte aérea nas doses baixas; o benefício de P foi maior nas variedades Sunrise Solo e Improved Sunrise Solo - Line 72/12, enquanto as variedades Baixinho de Santa Amália e Tainung nº 1 apresentaram benefício micorrízico proporcionalmente maior, resultando em maior eficiência simbiótica. As variedades de mamoeiro apresentaram a seguinte classificação quanto à dependência micorrízica: Baixinho de Santa Amália = Tainung nº 1 > Sunrise Solo = Improved Sunrise Solo - Line 72/12. A maior ou menor dependência relacionou-se com a capacidade da variedade em produzir raízes.Plant genome is an important factor in plant response to arbuscular fungi. This work was designed to evaluate the degree of response to P application and mycorrhizal dependence of different varieties of papaya (Carica papaya L.. The experiment was performed under greenhouse conditions at Embrapa-Centro Nacional de Pesquisa de Mandioca e Fruticultura, Cruz das Almas, BA, Brazil, using seedlings of four varieties, six rates of P: 0, 20, 40, 80, 140 and 280 mg dm-3, and three fungi

  18. Artificial neural network and response surface methodology modeling in mass transfer parameters predictions during osmotic dehydration of Carica papaya L.

    Directory of Open Access Journals (Sweden)

    J. Prakash Maran

    2013-09-01

    Full Text Available In this study, a comparative approach was made between artificial neural network (ANN and response surface methodology (RSM to predict the mass transfer parameters of osmotic dehydration of papaya. The effects of process variables such as temperature, osmotic solution concentration and agitation speed on water loss, weight reduction, and solid gain during osmotic dehydration were investigated using a three-level three-factor Box-Behnken experimental design. Same design was utilized to train a feed-forward multilayered perceptron (MLP ANN with back-propagation algorithm. The predictive capabilities of the two methodologies were compared in terms of root mean square error (RMSE, mean absolute error (MAE, standard error of prediction (SEP, model predictive error (MPE, chi square statistic (χ2, and coefficient of determination (R2 based on the validation data set. The results showed that properly trained ANN model is found to be more accurate in prediction as compared to RSM model.

  19. GROWTH OF MACROBRACHIUM ROSENBERGII FED WITH MANGO SEED KERNEL,BANANA PEEL AND PAPAYA PEEL INCORPORATED FEEDS

    Directory of Open Access Journals (Sweden)

    P. Aarumugam

    2013-06-01

    Full Text Available The growth promoting potential of fruits wastes, mango seed kernel, banana peel and papaya peel on the freshwater prawn, Macrobrachium rosenbergii post larvae (PL was evaluated. Basal diet equated to 35% protein was prepared by using soybean meal, groundnut oilcake, horse gram and wheat flour. Each fruit waste powder was separately incorporated with basal diet at a proportion of 10%. Sunflower oil was used as lipid source. Egg albumin and tapioca flour were used as binding agents. Vitamin B-complex with Vitamin-C was also mixed. Feed without any fruit waste was served as control. M. rosenbergii PL (length: 1.2-1.4 cm; weight: 0.09- 0.13 g was fed with these feeds for a period of 90 days. Significant improvements in the nutritional indices (survival rate, weight gain, biomass index, specific growth rate and condition factor, concentrations of biochemical constituents (total protein, carbohydrate and lipid, levels of non-enzymatic antioxidants (vitamin-C and E, content of minerals (Na+ and K+, activities of digestive enzymes (protease, amylase and lipase, and profiles of essential amino acids and fatty acids were recorded in fruits wastes incorporated feeds fed PL when compared with control (P< 0.003 – 0.878. The overall results indicated the fact that mango seed kernel incorporated feed was produced the best performance, followed by better performance of banana peel and good performance of papaya peel. These fruits wastes incorporated feeds enhance digestive enzymes activities and act as appetizer, which in turn enhances food utilization and ultimately yielded better survival and growth of M. rosenbergii PL. Therefore, these fruits wastes have considerable potentials in sustainable development of Macrobrachium culture.

  20. Early diagnosis of a Mexican variant of Papaya meleira virus (PMeV-Mx) by RT-PCR.

    Science.gov (United States)

    Zamudio-Moreno, E; Ramirez-Prado, J H; Moreno-Valenzuela, O A; Lopez-Ochoa, L A

    2015-02-06

    Papaya meleira disease was identified in Brazil in the 1980s. The disease is caused by a double-stranded RNA virus known as Papaya meleira virus (PMeV), which has also been recently reported in Mexico. However, previously reported PMeV primers failed to diagnose the Mexican form of the disease. A genomic approach was used to identify sequences of the Mexican virus isolate, referred here to as PMeV-Mx, to develop a diagnostic method. A mini cDNA library was generated using total RNA from the latex of fruits; this RNA was also sequenced using the Illumina platform. Sequences corresponding to the previously reported 669-base pair sequence for PMeV from Brazil (PMeV-Br) were identified within the PMeV-Mx genome, exhibiting 79-92% identity with PMeV-Br. In addition, a new sequence of 1154-base pairs encoding a putative RNA-dependent RNA polymerase was identified in PMeV-Mx. Primers designed against this sequence detected both virus isolates, 2 amplicons of 173 and 491 base pairs from PMeV-Br and PMeV-Mx, and shared 100 and 98% identity, respectively. PMeV-Mx was found in the latex of fruits, in seedlings, and in the leaves, flowers, petioles, and seeds of mature plants. PMeV-Mx was more abundant in the latex of fruits than in the leaves. The limit of detection of the CB38/CB39 primer pair was 1 fg and 1 pg using total RNA extracted from the latex of fruits and from seedlings, respectively. A sensitive and early diagnosis protocol was developed; this method will enable the certification of seeds and seedlings prior to transplantation to the field.