Quark (diquark) fragmentation in soft π-p interactions at P=40 GeV/c

International Nuclear Information System (INIS)

Didenko, L.A.; Grishin, V.G.; Kuznetsov, V.A.

1984-01-01

The quark and diquark fragmentation into π +- -, K 0 -mesons and Λ-hyperons in soft π - p-interactions at 40 GeV/c is studied. Fragmentation Dsup(πsup(+-)) (Xsub(F)) and invariant Fsup(πsup(+-)) (Xsub(F)) functions are compared with analogous data on ν(anti ν)p - interactions. It is shown that a good agreement exists in the region Xsub(F) > or approximately 0.15 for these different processes. The Xsub(E)-dependence of the quark and diquark fragmentation function for neutral kaons is similar to that in e + e - annihilation. The pickup probability of strange s(anti s) quark (lambda sub(s)) and diquark (lambda sub(qq)) relative to u(anti u) and d(anti d) quarks from the sea has been found to be equal to lambda sub(s)=0.17 and lambda sub(qq)=0.14+-0.03

Highly conserved serine residue 40 in HIV-1 p6 regulates capsid processing and virus core assembly

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Solbak Sara MØ

2011-02-01

Full Text Available Abstract Background The HIV-1 p6 Gag protein regulates the final abscission step of nascent virions from the cell membrane by the action of two late assembly (L- domains. Although p6 is located within one of the most polymorphic regions of the HIV-1 gag gene, the 52 amino acid peptide binds at least to two cellular budding factors (Tsg101 and ALIX, is a substrate for phosphorylation, ubiquitination, and sumoylation, and mediates the incorporation of the HIV-1 accessory protein Vpr into viral particles. As expected, known functional domains mostly overlap with several conserved residues in p6. In this study, we investigated the importance of the highly conserved serine residue at position 40, which until now has not been assigned to any known function of p6. Results Consistently with previous data, we found that mutation of Ser-40 has no effect on ALIX mediated rescue of HIV-1 L-domain mutants. However, the only feasible S40F mutation that preserves the overlapping pol open reading frame (ORF reduces virus replication in T-cell lines and in human lymphocyte tissue cultivated ex vivo. Most intriguingly, L-domain mediated virus release is not dependent on the integrity of Ser-40. However, the S40F mutation significantly reduces the specific infectivity of released virions. Further, it was observed that mutation of Ser-40 selectively interferes with the cleavage between capsid (CA and the spacer peptide SP1 in Gag, without affecting cleavage of other Gag products. This deficiency in processing of CA, in consequence, led to an irregular morphology of the virus core and the formation of an electron dense extra core structure. Moreover, the defects induced by the S40F mutation in p6 can be rescued by the A1V mutation in SP1 that generally enhances processing of the CA-SP1 cleavage site. Conclusions Overall, these data support a so far unrecognized function of p6 mediated by Ser-40 that occurs independently of the L-domain function, but selectively

Electronic structure of Pd42.5Ni7.5Cu30P20, an excellent bulk metallic glass former: Comparison to the Pd40Ni40P20 reference glass

International Nuclear Information System (INIS)

Hosokawa, S.; Sato, H.; Happo, N.; Mimura, K.; Tezuka, Y.; Ichitsubo, T.; Matsubara, E.; Nishiyama, N.

2007-01-01

In-house photoemission and inverse-photoemission spectra (PES and IPES) were measured on Pd 42.5 Ni 7.5 Cu 30 P 20 and Pd 40 Ni 40 P 20 bulk metallic glasses in order to clarify the origin of excellent glass-forming ability from the viewpoint of electronic structure. Minima are observed for both the metallic glasses at a slightly higher energy than the Fermi level. Incident photon-energy dependent PES spectra were obtained using synchrotron radiation and the Pd 4d partial density of states (DOS) was estimated from the PES data. Soft X-ray emission spectra were also measured near the Ni and Cu 2p 3/2 absorption edges to evaluate, respectively, the Ni and Cu 3d partial DOS in the valence band. The Pd 4d and the Ni and Cu 3d partials in the conduction band were obtained from X-ray absorption spectra around the Pd 3p 3/2 and Ni and Cu 2p 3/2 absorption edges, respectively. It was found that the Pd 4d partial DOS near the Fermi energy largely decreases and becomes localized by replacing the Ni atoms with the Cu atoms, which may be closely related to the excellent glass-forming ability of the Pd 42.5 Ni 7.5 Cu 30 P 20 bulk metallic glass due to a selective formation of Pd-P covalent bonds

Crystallization of Pd40CU30Ni10P20 bulk metallic glass with and without pressure

DEFF Research Database (Denmark)

Yang, B.; Jiang, Jianzhong; Zhuang, Yanxin

2007-01-01

The glass-transition behavior of Pd40Cu30Ni10P20 bulk metallic glass was investigated by differential scanning calorimetry (DSC) and X-ray powder diffraction (XRD). The effect of pressure on the crystallization behavior of Pd40Cu30Ni10P20 bulk glass was studied by in situ high-pressure and high...

The secreted form of the p40 subunit of interleukin (IL)-12 inhibits IL-23 functions and abrogates IL-23-mediated antitumour effects

Science.gov (United States)

Shimozato, Osamu; Ugai, Shin-ichi; Chiyo, Masako; Takenobu, Hisanori; Nagakawa, Hiroyasu; Wada, Akihiko; Kawamura, Kiyoko; Yamamoto, Hiroshi; Tagawa, Masatoshi

2006-01-01

Interleukin (IL)-23 is a heterodimeric cytokine consisting of a novel p19 molecule and the p40 subunit of IL-12. Since secreted p40 can act as an antagonist for IL-12, we investigated whether p40 also inhibited IL-23-mediated immunological functions. p40 did not induce interferon (IFN)-γ or IL-17 production from splenocytes but impaired IL-23-induced cytokine production by competitive binding to the IL-23 receptors. Furthermore, a mixed population of murine colon carcinoma Colon 26 cells transduced with the p40 gene and those transduced with the IL-23 gene developed tumours in syngenic mice, whereas the IL-23-expressing Colon 26 cells were completely rejected. p40 also suppressed IFN-γ production of antigen-stimulated splenocytes and IL-23-mediated cytotoxic T-lymphocyte activities in the mice that rejected Colon 26 cells expressing IL-23. p40 can thereby antagonize IL-23 and is a possible therapeutic agent for suppression of IL-23 functions. PMID:16423037

High throughput testing of the SV40 Large T antigen binding to cellular p53 identifies putative drugs for the treatment of SV40-related cancers

International Nuclear Information System (INIS)

Carbone, Michele; Rudzinski, Jennifer; Bocchetta, Maurizio

2003-01-01

SV40 has been linked to some human malignancies, and the evidence that this virus plays a causative role in mesothelioma and brain tumors is mounting. The major SV40 oncoprotein is the Large tumor antigen (Tag). A key Tag transforming activity is connected to its capability to bind and inactivate cellular p53. In this study we developed an effective, high throughput, ELISA-based method to study Tag-p53 interaction in vitro. This assay allowed us to screen a chemical library and to identify a chemical inhibitor of the Tag binding to p53. We propose that our in vitro assay is a useful method to identify molecules that may be used as therapeutic agents for the treatment of SV40-related human cancers

Decreased D2-40 and increased p16INK4A immunoreactivities correlate with higher grade of cervical intraepithelial neoplasia

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Lu Zhouping

2011-07-01

Full Text Available Abstract Background D2-40 has been shown a selective marker for lymphatic endothelium, but also shown in the benign cervical basal cells. However, the application of D2-40 immunoreactivity in the cervical basal cells for identifying the grade of cervical intraepithelial neoplasia (CIN has not been evaluated. Methods In this study, the immunoreactive patterns of D2-40, compared with p16INK4A, which is currently considered as the useful marker for cervical cancers and their precancerous diseases, were examined in total 125 cervical specimens including 32 of CIN1, 37 of CIN2, 35 of CIN3, and 21 of normal cervical tissue. D2-40 and p16INK4A immunoreactivities were scored semiquantitatively according to the intensity and/or extent of the staining. Results Diffuse D2-40 expression with moderate-to-strong intensity was seen in all the normal cervical epithelia (21/21, 100% and similar pattern of D2-40 immunoreactivity with weak-to-strong intensity was observed in CIN1 (31/32, 97.2%. However, negative and/or focal D2-40 expression was found in CIN2 (negative: 20/37, 54.1%; focal: 16/37, 43.2% and CIN3 (negative: 22/35, 62.8%; focal: 12/35, 34.3%. On the other hand, diffuse immunostaining for p16INK4A was shown in 37.5% of CIN1, 64.9% of CIN2, and 80.0% of CIN3. However, the immunoreactive pattern of D2-40 was not associated with the p16INK4A immunoreactivity. Conclusions Immunohistochemical analysis of D2-40 combined with p16INK4A may have a significant implication in clinical practice for better identifying the grade of cervical intraepithelial neoplasia, especially for distinguishing CIN1 from CIN2/3.

Ultrasound-induced crystallization around the glass transition temperature for Pd40Ni40P20 metallic glass

International Nuclear Information System (INIS)

Ichitsubo, Tetsu; Matsubara, Eiichiro; Kai, Satoshi; Hirao, Masahiko

2004-01-01

We have found that crystallization of a Pd 40 Ni 40 P 20 bulk metallic glass is accelerated in the vicinity of the glass transition temperature T g when it is subjected to sub/low-MHz frequency ultrasonic vibration. Resonance frequencies and internal frictions have been measured with the electromagnetic acoustic resonance (EMAR) technique. In the initial heating process of an as-cast glassy sample, the resonance frequencies jump up just above T g under ultrasonic excitation, which is attributed to nano-crystallization that is confirmed by the X-ray diffraction profile. However, such a notable change is not observed without ultrasonic vibration. The irregular Λ-shaped internal-friction peaks are also observed prior to the abrupt crystallization. This rapid crystallization is considered to be caused by a stochastic resonance, in which the jump frequency of atoms matches the frequency of the interatomic-potential change by the ultrasonic vibration

An InGaAs/InP 40 GHz CML static frequency divider

International Nuclear Information System (INIS)

Su Yongbo; Jin Zhi; Cheng Wei; Ge Ji; Wang Xiantai; Chen Gaopeng; Liu Xinyu; Xu Anhuai; Qi Ming

2011-01-01

Static frequency dividers are widely used as a circuit performance benchmark or figure-of-merit indicator to gauge a particular device technology's ability to implement high speed digital and integrated high performance mixed-signal circuits. We report a 2 : 1 static frequency divider in InGaAs/InP heterojunction bipolar transistor technology. This is the first InP based digital integrated circuit ever reported on the mainland of China. The divider is implemented in differential current mode logic (CML) with 30 transistors. The circuit operated at a peak clock frequency of 40 GHz and dissipated 650 mW from a single -5 V supply. (semiconductor integrated circuits)

Measurement of negatively charged pion spectra in inelastic p+p interactions at $p_{lab}$ = 20, 31, 40, 80 and 158 GeV/c

CERN Document Server

Abgrall, N; Ali, Y; Anticic, T; Antoniou, N; Baatar, B; Bay, F; Blondel, A; Blumer, J; Bogomilov, M; Bravar, A; Brzychczyk, J; Bunyatov, S A; Busygina, O; Christakoglou, P; Czopowicz, T; Davis, N; Debieux, S; Dembinski, H; Diakonos, F; Di Luise, S; Dominik, W; Drozhzhova, T; Dumarchez, J; Dynowski, K; Engel, R; Ereditato, A; Feofilov, G A; Fodor, Z; Fulop, A; Gazdzicki, M; Golubeva, M; Grebieszkow, K; Grzeszczuk, A; Guber, F; Haesler, A; Hasegawa, T; Hierholzer, M; Idczak, R; Igolkin, S; Ivashkin, A; Jokovic, D; Kadija, K; Kapoyannis, A; Katrynska, N; Kaptur, E; Kielczewska, D; Kirejczyk, M; Kisiel, J; Kiss, T; Kleinfelder, S; Kobayashi, T; Kolesnikov, V I; Kolev, D; Kondratiev, V P; Korzenev, A; Kovesarki, P; Kowalski, S; Krasnoperov, A; Kurepin, A; Larsen, D; Laszlo, A; Lyubushkin, V V; Mackowiak-Pawlowska, M; Majka, Z; Maksiak, B; Malakhov, A I; Manic, D; Marcinek, A; Marin, V; Marton, K; Mathes, H J; Matulewicz, T; Matveev, V; Melkumov, G.L; Mrowczynski, St; Murphy, S; Nakadaira, T; Nirkko, M; Nishikawa, K; Palczewski, T; Palla, G; Panagiotou, A D; Paul, T; Pistillo, C; Peryt, W; Petukhov, O; Planeta, R; Pluta, J; Popov, B A; Posiadala, M; Pulawski, S; Puzovic, J; Rauch, W; Ravonel, M; Redij, A; Renfordt, R; Robert, A; Rohrich, D; Rondio, E; Roth, M; Rubbia, A; Rustamov, A; Rybczynski, M; Sadovsky, A; Sakashita, K; Savic, M; Schmidt, K; Sekiguchi, T; Seyboth, P; Sgalaberna, D; Shibata, M; Sipos, R; Skrzypczak, E; Slodkowski, M; Staszel, P; Stefanek, G; Stepaniak, J; Strobele, H; Susa, T; Szuba, M; Tada, M; Tereshchenko, V; Tolyhi, T; Tsenov, R; Turko, L; Ulrich, R; Unger, M; Vassiliou, M; Veberic, D; Vechernin, V V; Vesztergombi, G; Vinogradov, L; Wilczek, A; Wlodarczyk, Z; Wojtaszek-Szwarc, A; Wyszynski, O; Zambelli, L; Zipper, W

2014-01-01

We present experimental results on inclusive spectra and mean multiplicities of negatively charged pions produced in inelastic p+p interactions at incident projectile momenta of 20, 31, 40, 80 and 158GeV/c ($\\sqrt{s}$ = 6.3, 7.7, 8.8, 12.3 and 17.3GeV, respectively). The measurements were performed using the large acceptance NA61/SHINE hadron spectrometer at the CERN super proton synchrotron. Two-dimensional spectra are determined in terms of rapidity and transverse momentum. Their properties such as the width of rapidity distributions and the inverse slope parameter of transverse mass spectra are extracted and their collision energy dependences are presented. The results on inelastic p+p interactions are compared with the corresponding data on central Pb+Pb collisions measured by the NA49 experiment at the CERN SPS. The results presented in this paper are part of the NA61/SHINE ion program devoted to the study of the properties of the onset of deconfinement and search for the critical point of strongly inter...

Preliminary results of sup(40)Ca(e,e'c) reaction analysis c p,α, based on statistical model

International Nuclear Information System (INIS)

Herdade, S.B.; Emrich, H.J.

1990-01-01

Statistical model calculations relative to the reactions sup(40)Ca (e,e'p) sup(39)K and sup(40)Ca(e,e'P sub(o)) sup(39)K sup(gs), using a modified version of the program STAPRE are compared with experimental results obtained from coincidence experiments carried out at the Mainz microtron MAMI A. Preliminary results indicate that the statistical decay of a 1 sup(-) level in the sup(40)Ca compound nucleus, at an excitation energy + 20 MeV, to the ground state of the sup(39)K residual nucleus is only about 15% of the total decay, indicating that direct and/or semi-direct mechanisms contribute to the major part of the decay. (author)

Structural stability of Pd40Cu30Ni10P20 metallic glass in supercooled liquid region

International Nuclear Information System (INIS)

Jiang, J.Z.; Saksl, K.

2004-01-01

Phase separation of bulk and ribbon Pd 40 Cu 30 Ni 10 P 20 glasses, annealed in the supercooled liquid region at ambient pressure and high pressures, has been studied by means of differential scanning calorimetry (DSC) and X-ray diffraction techniques. DSC measurements show only one glass transition event in all annealed samples, indicating that no phase separation occurs in the alloy annealed in the supercooled liquid region. Phase analyses reveal at least six crystalline phases in the crystallized sample: monoclinic, tetragonal Cu 3 Pd-like, rhombohedral, fcc-Ni 2 Pd 2 P, fcc-(Ni, Pd) solid solution, and body-centered tetragonal (bct) Ni 3 P-like phases. Annealing treatments under external pressures in the vicinity of the glass transition temperature neither induce phase separation nor alter the glass transition temperature of the Pd 40 Cu 30 Ni 10 P 20 bulk glass

Nfkb1 inhibits LPS-induced IFN-β and IL-12 p40 production in macrophages by distinct mechanisms.

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Xixing Zhao

Full Text Available Nfkb1-deficient murine macrophages express higher levels of IFN-β and IL-12 p40 following LPS stimulation than control macrophages, but the molecular basis for this phenomenon has not been completely defined. Nfkb1 encodes several gene products including the NF-κB subunit p50 and its precursor p105. p50 is derived from the N-terminal of 105, and p50 homodimers can exhibit suppressive activity when overexpressed. The C-terminal region of p105 is necessary for LPS-induced ERK activation and it has been suggested that ERK activity inhibits both IFN-β and IL-12 p40 following LPS stimulation. However, the contributions of p50 and the C-terminal domain of p105 in regulating endogenous IFN-β(Ifnb and IL-12 p40 (Il12b gene expression in macrophages following LPS stimulation have not been directly compared.We have used recombinant retroviruses to express p105, p50, and the C-terminal domain of p105 (p105ΔN in Nfkb1-deficient murine bone marrow-derived macrophages at near endogenous levels. We found that both p50 and p105ΔN inhibited expression of Ifnb, and that inhibition of Ifnb by p105ΔN depended on ERK activation, because a mutant of p105ΔN (p105ΔNS930A that lacks a key serine necessary to support ERK activation failed to inhibit. In contrast, only p105ΔN but not p50 inhibited Il12b expression. Surprisingly, p105ΔNS930A retained inhibitory activity for Il12b, indicating that ERK activation was not necessary for inhibition. The differential effects of p105ΔNS930A on Ifnb and Il12b expression inversely correlated with the function of one of its binding partners, c-Rel. This raised the possibility that p105ΔNS930A influences gene expression by interfering with the function of c-Rel.These results demonstrate that Nfkb1 exhibits multiple gene-specific inhibitory functions following TLR stimulation of murine macrophages.

Expression of p40 (∆Np63) protein in meningiomas, an unexpected finding: immunohistochemical study and evaluation of its possible prognostic role.

Science.gov (United States)

Guadagno, Elia; Del Basso De Caro, Marialaura; Pignatiello, Sara; Sciammarella, Concetta; Solari, Domenico; Cappabianca, Paolo; Maiuri, Francesco; Dones, Flavia

2016-09-01

According to the 2007 WHO (World Health Organization) Classification, meningiomas are divided into three grades of malignancy, with different recurrence rate, based exclusively on histopathological parameters. Loss/reduction of PgR (Progesterone Receptor) expression and increased Ki67 L.I. (Labeling Index) have been proven as possible prognostic factors able to predict the relapse of the disease. However, they sometimes result unreliable, especially when discordant. p40 is the short form of the p53 homologue gene p63, also named ∆Np63, and its antibody has recently been introduced as a highly specific diagnostic marker of the squamous cell carcinoma of the lung. Nevertheless its expression has been found in many other unconventional sites (e.g. placenta, urotheluim, etc). Herein we assessed the immuno-expression of p40 protein in a series of 72 meningiomas (35 grade I and 37 grade II) and analyzed its correlation with clinicopathological parameters, overall survival and recurrence free interval. We found that a high p40 score correlated with high histological grade, presence of recurrence, increased Ki67 L.I. and loss/reduction of PgR signal. Moreover, a higher expression of p40 was shown to be a significant prognostic factor for the development of recurrences and resulted a prognostic independent variable in multivariate analysis. Overall, for the first time, we investigated the expression of p40 protein in meningiomas and explored its usefulness as prognostic marker in addition to PgR and Ki67 L.I.

A lactobacillus rhamnosus GG-derived soluble protein, p40, stimulates ligand release from intestinal epithelial cells to transactivate epidermal growth factor receptor

Science.gov (United States)

Protein p40, a Lactobacillus rhamnosus GG (LGG)-derived soluble protein, ameliorates intestinal injury and colitis, reduces apoptosis and preserves barrier function by activation of EGF receptor (EGFR) in intestinal epithelial cells. The aim of this study was to determine the mechanisms by which p40...

Measurement of negatively charged pion spectra in inelastic p+p interactions at p{sub lab} = 20, 31, 40, 80 and 158 GeV/c

Energy Technology Data Exchange (ETDEWEB)

Abgrall, N.; Blondel, A.; Bravar, A.; Debieux, S.; Haesler, A.; Korzenev, A.; Murphy, S.; Ravonel, M. [University of Geneva, Geneva (Switzerland); Aduszkiewicz, A.; Dominik, W.; Kielczewska, D.; Kirejczyk, M.; Matulewicz, T.; Posiadala, M.; Skrzypczak, E. [University of Warsaw, Faculty of Physics, Warsaw (Poland); Ali, Y.; Brzychczyk, J.; Majka, Z.; Marcinek, A.; Planeta, R.; Staszel, P.; Wyszynski, O. [Jagiellonian University, Cracow (Poland); Anticic, T.; Kadija, K.; Susa, T. [Rudjer Boskovic Institute, Zagreb (Croatia); Antoniou, N.; Christakoglou, P.; Davis, N.; Diakonos, F.; Kapoyannis, A.; Panagiotou, A.D.; Vassiliou, M. [University of Athens, Athens (Greece); Baatar, B.; Bunyatov, S.A.; Kolesnikov, V.I.; Krasnoperov, A.; Lyubushkin, V.V.; Malakhov, A.I.; Melkumov, G.L.; Tereshchenko, V. [Joint Institute for Nuclear Research, Dubna (Russian Federation); Bay, F.; Luise, S.Di; Rubbia, A.; Sgalaberna, D. [ETH, Zurich (Switzerland); Blumer, J.; Dembinski, H.; Engel, R.; Mathes, H.J.; Roth, M.; Szuba, M.; Ulrich, R.; Unger, M. [Karlsruhe Institute of Technology, Karlsruhe (Germany); Bogomilov, M.; Kolev, D.; Tsenov, R. [University of Sofia, Faculty of Physics, Sofia (Bulgaria); Busygina, O.; Golubeva, M.; Guber, F.; Ivashkin, A.; Kurepin, A.; Marin, V.; Petukhov, O.; Sadovsky, A. [Institute for Nuclear Research, Moscow (Russian Federation); Czopowicz, T.; Dynowski, K.; Grebieszkow, K.; Maksiak, B.; Peryt, W.; Pluta, J.; Slodkowski, M. [Warsaw University of Technology, Warsaw (Poland); Drozhzhova, T.; Feofilov, G.A.; Igolkin, S.; Kondratiev, V.P.; Vechernin, V.V.; Vinogradov, L. [St. Petersburg State University, St. Petersburg (Russian Federation); Dumarchez, J.; Robert, A.; Zambelli, L. [LPNHE, University of Paris VI and VII, Paris (France); Ereditato, A.; Hierholzer, M.; Nirkko, M.; Pistillo, C.; Redij, A. [University of Bern, Bern (Switzerland); Fodor, Z.; Fulop, A.; Kiss, T.; Laszlo, A.; Marton, K.; Palla, G.; Sipos, R.; Tolyhi, T.; Vesztergombi, G. [KFKI Research Institute for Particle and Nuclear Physics, Budapest (Hungary); Gazdzicki, M. [Jan Kochanowski University in Kielce, Kielce (Poland); University of Frankfurt, Frankfurt (Germany); Grzeszczuk, A.; Kaptur, E.; Kisiel, J.; Kowalski, S.; Larsen, D.; Pulawski, S.; Schmidt, K.; Wilczek, A.; Zipper, W. [University of Silesia, Katowice (Poland); Hasegawa, T.; Kobayashi, T.; Nakadaira, T.; Nishikawa, K.; Sakashita, K.; Sekiguchi, T.; Shibata, M.; Tada, M. [High Energy Accelerator Research Organization (KEK), Tsukuba, Ibaraki (Japan); Idczak, R.; Kovesarki, P.; Turko, L. [University of Wroclaw, Wroclaw (Poland); Jokovic, D.; Manic, D.; Puzovic, J.; Savic, M. [University of Belgrade, Belgrade (Serbia); Kleinfelder, S. [University of California, Irvine (United States); Mackowiak-Pawlowska, M.; Renfordt, R.; Rustamov, A.; Stroebele, H. [University of Frankfurt, Frankfurt (Germany); Matveev, V. [Joint Institute for Nuclear Research, Dubna (Russian Federation); Institute for Nuclear Research, Moscow (Russian Federation); Mrowczynski, S.; Rybczynski, M.; Seyboth, P.; Stefanek, G.; Wlodarczyk, Z.; Wojtaszek-Szwarc, A. [Jan Kochanowski University in Kielce, Kielce (Poland); Palczewski, T.; Rondio, E.; Stepaniak, J. [National Centre for Nuclear Research, Warsaw (Poland); Paul, T.; Veberic, D. [University Nova Gorica, Laboratory of Astroparticle Physics, Nova Gorica (Slovenia); Popov, B.A. [Joint Institute for Nuclear Research, Dubna (Russian Federation); LPNHE, University of Paris VI and VII, Paris (France); Rauch, W. [Fachhochschule Frankfurt, Frankfurt (Germany); Roehrich, D. [University of Bergen, Bergen (Norway); Collaboration: NA61/SHINE Collaboration

2014-03-15

We present experimental results on inclusive spectra and mean multiplicities of negatively charged pions produced in inelastic p+p interactions at incident projectile momenta of 20, 31, 40, 80 and 158 GeV/c (√(s) = 6.3, 7.7, 8.8, 12.3 and 17.3 GeV, respectively). The measurements were performed using the large acceptance NA61/SHINE hadron spectrometer at the CERN super proton synchrotron. Two-dimensional spectra are determined in terms of rapidity and transverse momentum. Their properties such as the width of rapidity distributions and the inverse slope parameter of transverse mass spectra are extracted and their collision energy dependences are presented. The results on inelastic p+p interactions are compared with the corresponding data on central Pb+Pb collisions measured by the NA49 experiment at the CERN SPS. The results presented in this paper are part of the NA61/SHINE ion program devoted to the study of the properties of the onset of deconfinement and search for the critical point of strongly interacting matter. They are required for interpretation of results on nucleus-nucleus and proton-nucleus collisions. (orig.)

Polarization measurements for P-12C elastic scattering between 40-75 MeV

International Nuclear Information System (INIS)

Kato, S.; Okada, K.; Kondo, M.; Shimizu, A.; Hosono, K.; Saito, T.; Matsuoka, N.; Nagamachi, S.; Nisimura, K.; Tamura, N.

1980-01-01

Absolute values of the polarization in p- 12 C elastic scattering have been measured at 60.0 and 64.5 MeV at 47.5 0 in the laboratory system using a double scattering method and a method to measure an asymmetry with a polarized beam. The results are P(60.0 MeV, 47.5 0 ) = 0.965 +- 0.011 and P(64.5 MeV, 47.5 0 ) = 0.975 +- 0.011. Based on these values, the polarization measurements have been extended to the energy range from 40-75 MeV at several angles around 47.5 0 using the polarized proton beam and the energy degrader. Differential cross sections and polarizations from 15-115 0 are also presented at 65 MeV. These data will be valuable for the monitoring of beam polarization in this energy region. (orig.)

pKa values of hyodeoxycholic and cholic acids in the binary mixed micelles sodium-hyodeoxycholate-Tween 40 and sodium-cholate-Tween 40: Thermodynamic stability of the micelle and the cooperative hydrogen bond formation with the steroid skeleton.

Science.gov (United States)

Poša, Mihalj; Pilipović, Ana; Bećarević, Mirjana; Farkaš, Zita

2017-01-01

Due to a relatively small size of bile acid salts, their mixed micelles with nonionic surfactants are analysed. Of the special interests are real binary mixed micelles that are thermodynamically more stable than ideal mixed micelles. Thermodynamic stability is expressed with an excess Gibbs energy (G E ) or over an interaction parameter (β ij ). In this paper sodium salts of cholic (C) and hyodeoxycholic acid (HD) in their mixed micelles with Tween 40 (T40) are analysed by potentiometric titration and their pKa values are determined. Examined bile acids in mixed micelles with T40 have higher pKa values than free bile acids. The increase of ΔpKa acid constant of micellary bound C and HD is in a correlation with absolute values of an interaction parameter. According to an interaction parameter and an excess Gibbs energy, mixed micelle HD-T40 are thermodynamically more stable than mixed micelles C-T40. ΔpKa values are higher for mixed micelles with Tween 40 whose second building unit is HD, related to the building unit C. In both micellar systems, ΔpKa increases with the rise of a molar fraction of Tween 40 in binary mixtures of surfactants with sodium salts of bile acids. This suggests that, ΔpKa can be a measure of a thermodynamic stabilization of analysed binary mixed micelles as well as an interaction parameter. ΔpKa values are confirmed by determination of a distribution coefficient of HD and C in systems: water phase with Tween 40 in a micellar concentration and 1-octanol, with a change of a pH value of a water phase. Conformational analyses suggests that synergistic interactions between building units of analysed binary micelles originates from formation of hydrogen bonds between steroid OH groups and polyoxyethylene groups of the T40. Relative similarity and spatial orientation of C 3 and C 6 OH group allows cooperative formation of hydrogen bonds between T40 and HD - excess entropy in formation of mixed micelle. If a water solution of analysed binary

Secondary particle multiplicity depending on the collisions of π--mesons with 12C nucleons at p=40 GeV/c

International Nuclear Information System (INIS)

Angelov, N.S.; Grishin, V.G.; Nad', Yu.; Kanarek, T.; Batskovich, S.

1978-01-01

The multiplicities of secondary charged particles in π -12 C-interactions with ν-protons of carbon nucleus at p=40 GeV/c (ν=1-5) is presented. It is shown that the dependence of the average values of particle multiplicities on ν approximately equals the dependence on initial pion energy in π - p-interactions in the range from 40 to 200 GeV. KNO scaling and the Wroblewski empirical law for interactions with several nucleons of 12 C nucleus are observed

Response of metallic glasses Fe40Ni40P14B6 and Fe80B20 to irradiation with 800-MeV protons

International Nuclear Information System (INIS)

Cost, J.R.; Sommer, W.F.

1981-01-01

Metallic glasses with compositions of Fe 40 Ni 40 P 14 B 6 and Fe 80 B 20 were irradiated in the 800 MeV proton beam at the Los Alamos Meson Physics Facility while the electrical resistance and length changes were monitored. The resistance and the length of the first alloy were both found to increase and saturate with dose to ΔR/R approx. = 5 x 10 -3 and ΔL/L approx. = 2 x 10 -3 . For the second alloy the total dose of 1.1 x 10 19 p/cm 2 , which was calculated to give roughly 0.12 dpa, was slightly less than that required for saturation. No annealing of these increases was observed for anneals from room temperature to 250 0 C. These results are interpreted in terms of a model in which collision cascades create small regions of increased atomic disorder which fully overlap each other at saturation

40 CFR 704.33 - P-tert-butylbenzoic acid (P-TBBA), p-tert-butyltoluene (P-TBT) and p-tert-butylbenzaldehyde (P-TBB).

Science.gov (United States)

2010-07-01

...-tert-butyltoluene (P-TBT) and p-tert-butylbenzaldehyde (P-TBB). 704.33 Section 704.33 Protection of... (P-TBBA), p-tert-butyltoluene (P-TBT) and p-tert-butylbenzaldehyde (P-TBB). (a) Definitions. (1) P..., CAS No. 98-73-7. (2) P-TBT means the substance p-tert-butyltoluene, also identified as 1-(1,1...

Isotope shift of 40,42,44,48Ca in the 4s 2S1/2 → 4p 2P3/2 transition

Science.gov (United States)

Gorges, C.; Blaum, K.; Frömmgen, N.; Geppert, Ch; Hammen, M.; Kaufmann, S.; Krämer, J.; Krieger, A.; Neugart, R.; Sánchez, R.; Nörtershäuser, W.

2015-12-01

We report on improved isotope shift measurements of the isotopes {}{40,42,{44,48}}Ca in the 4{{s}}{ }2{{{S}}}1/2\\to 4{{p}}{ }2{{{P}}}3/2 (D2) transition using collinear laser spectroscopy. Accurately known isotope shifts in the 4{{s}}{ }2{{{S}}}1/2\\to 4{{p}}{ }2{{{P}}}1/2(D1) transition were used to calibrate the ion beam energy with an uncertainty of {{Δ }}U≈ +/- 0.25 {{V}}. The accuracy in the D2 transition was improved by a factor of 5-10. A King-plot analysis of the two transitions revealed that the field shift factor in the D2 line is about 1.8(13)% larger than in the D1 transition which is ascribed to relativistic contributions of the 4{{{p}}}1/2 wave function.

Seventeen copies of the human 37 kDa laminin receptor precursor/p40 ribosome-associated protein gene are processed pseudogenes arisen from retropositional events

DEFF Research Database (Denmark)

Jackers, P; Clausse, N; Fernandez, M

1996-01-01

A cDNA coding for a 37 kDa polypeptide has been identified in several species as both the potential precursor of the 67 kDa laminin receptor (37LRP) and a putative ribosome-associated protein (p40). Interestingly, increased expression of this polypeptide (37LRP/p40) is consistently observed...

Interplay between PTB and miR-1285 at the p53 3'UTR modulates the levels of p53 and its isoform Δ40p53α.

Science.gov (United States)

Katoch, Aanchal; George, Biju; Iyyappan, Amrutha; Khan, Debjit; Das, Saumitra

2017-09-29

p53 and its translational isoform Δ40p53 are involved in many important cellular functions like cell cycle, cell proliferation, differentiation and metabolism. Expression of both the isoforms can be regulated at different steps. In this study, we explored the role of 3'UTR in regulating the expression of these two translational isoforms. We report that the trans acting factor, Polypyrimidine Tract Binding protein (PTB), also interacts specifically with 3'UTR of p53 mRNA and positively regulates expression of p53 isoforms. Our results suggest that there is interplay between miRNAs and PTB at the 3'UTR under normal and stress conditions like DNA damage. Interestingly, PTB showed some overlapping binding regions in the p53 3'UTR with miR-1285. In fact, knockdown of miR-1285 as well as expression of p53 3'UTR with mutated miR-1285 binding sites resulted in enhanced association of PTB with the 3'UTR, which provides mechanistic insights of this interplay. Taken together, the results provide a plausible molecular basis of how the interplay between miRNAs and the PTB protein at the 3'UTR can play pivotal role in fine tuning the expression of the two p53 isoforms. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

The proton spectral function of 40Ca and 48Ca studied with the (e,e'p) reaction

International Nuclear Information System (INIS)

Kramer, G.J.

1990-01-01

This thesis presents the results of an experimental study into the occupation of the orbitals around the Fermi level for 40 Ca and 48 Ca with quasi-elastic proton knock-out (e,e'p). Experiments have been carried out with the 500 MeV electron beam of the linear accelerator MEA at NIKHEF, Amsterdam. For 40 Ca the mechanism of the (e,e'p) reaction has been studied by comparing the measured momentum distributions of some strong transitions to discrete states in 39K , with various theoretical calculations. From this it has been concluded that uncertainties caused by deviations of the impulse approximation can be minimized if the measurements are carried out under parallel kinematical conditions. The spectroscopic strengths of the shell-model orbitals in states just below the Fermi level, for 40 Ca the 1d 3/2 , 1d 5/2 and 2s 1/2 orbitals, turned out to amount 50 to 70% of the IPSM limit. A small part of the missing strength has been found in the 1f 7/2 and 2p 3/2 orbitals which are just above the Fermi level (resp. 11 and 2% of the 2j+1 limit), which is an indication for ground state correlations. The spectroscopic strengths for the 1d 3/2 , 2s 1/2 and 1d 3/2 orbitals of 48 Ca turned out to be the same as for 40C a within the actual measuring accuracy. Above the Fermi level only strength in the 1f 7/2 orbital has been found (1% of the 2j+1 limit). The spectroscopic strengths determined with (e,e'p) experiments are about a factor two smaller than those obtained from (d, 3 He) experiments. This discrepancy has been studied by reviewing the model dependency of the DWBA analysis for the (d, 3 He) reaction with special emphasis on the sensitivities of the spectroscopic factors to the various approximations made in this theory. It is also investigated which part of the bound state wave function is probed by the (e,e'p) and the (d, 3 He) reactions in order to understand the model sensitivities arising from the exact shape of the bound state wave function. (H.W.).97 refs.; 48

Electron impact excitation of helium: A ploarization correlation study of the 31P state at 40 eV incident energy

International Nuclear Information System (INIS)

Harris, C.L.; Dorio, L.A.; Neill, P.A.

1996-01-01

Recently the Convergent Close-Coupling calculations, (CCC), of Fursa and Bray have been very successful predicting the behavior of the electron impact coherence parameters (EICP) for electron impact excitation of helium. In the present experimental study the linear Stokes parameters P 1 and P 2 have been measured for He(3 1 P) excitation using the polarization correlation technique. Data will be presented for electron impact energies of 40eV and 50eV. At present no other experimental data is available at 40eV. At 50eV angular correlation data measured using the VUV 3 1 P-1 1 S photons are available only out to a maximum electron scattering angle of 85 degrees. Due to the disadvantageous differential cross section and 40:1 branching ratio in favor of the VUV decay, the uncertainties in the present data are large. However, at selected electron scattering angles they are sufficient to distinguish the lack of convergence of the CCC predictions for the 69 state calculations (CCC69) in comparison with the 75 state model (CCC75). In particular at 50 eV incident electron energy and 120 degrees scattering angle the charge cloud alignment angles predicted by the two calculations differ by 90 degrees

Identification of p53 unbound to T-antigen in human cells transformed by simian virus 40 T-antigen.

Science.gov (United States)

O'Neill, F J; Hu, Y; Chen, T; Carney, H

1997-02-27

In several clones of SV40-transformed human cells, we investigated the relative amounts of large T-Antigen (T-Ag) and p53 proteins, both unbound and associated within complexes, with the goal of identifying changes associated with transformation and immortalization. Cells were transformed by wild type (wt) T-Ag, a functionally temperature sensitive T-Ag (tsA58) and other T-Ag variants. Western analysis showed that while most of the T-Ag was ultimately bound by p53, most of the p53 remained unbound to T-Ag. Unbound p53 remained in the supernatant after a T-Ag immunoprecipitation and p53 was present in two to fourfold excess of T-Ag. In one transformant there was five to tenfold more p53 than T-Ag. p53 was present in transformants in amounts at least 200-fold greater than in untransformed human cells. In wt and variant T-Ag transformants, including those generated with tsA58 T-Ag, large amounts of unbound p53 were present in both pre-crisis and immortal cells and when the cells were grown at permissive or non-permissive temperatures. We also found that in transformants produced by tsA58, an SV40/JCV chimeric T-Ag and other variants, T-Ag appeared to form a complex with p53 slowly perhaps because one or both proteins matured slowly. The presence in transformed human cells of large amounts of unbound p53 and in excess of T-Ag suggests that sequestration of p53 by T-Ag, resulting from complex formation, is required neither for morphological transformation nor immortalization of human cells. Rather, these results support the proposal that high levels of p53, the T-Ag/p53 complexes, or other biochemical event(s), lead to transformation and immortalization of human cells by T-Ag.

Nanostructure analysis of friction welded Pd-Ni-P/Pd-Cu-Ni-P metallic glass interface

International Nuclear Information System (INIS)

Ohkubo, T.; Shoji, S.; Kawamura, Y.; Hono, K.

2005-01-01

Friction welded Pd 40 Ni 40 P 20 /Pd 40 Cu 30 Ni 10 P 20 metallic glass interface has been characterized by energy filtering transmission electron microscopy. The interface is fully amorphous with a gradual compositional change of Cu and Ni in the range of 30 nm. By annealing above T g , the interdiffusion of Cu and Ni progressed in the supercooled liquid region, and the crystallization occurred from the Pd 40 Ni 40 P 20 glass

Interplay between PTB and miR-1285 at the p53 3′UTR modulates the levels of p53 and its isoform Δ40p53α

Science.gov (United States)

Katoch, Aanchal; George, Biju; Iyyappan, Amrutha; Khan, Debjit

2017-01-01

Abstract p53 and its translational isoform Δ40p53 are involved in many important cellular functions like cell cycle, cell proliferation, differentiation and metabolism. Expression of both the isoforms can be regulated at different steps. In this study, we explored the role of 3′UTR in regulating the expression of these two translational isoforms. We report that the trans acting factor, Polypyrimidine Tract Binding protein (PTB), also interacts specifically with 3′UTR of p53 mRNA and positively regulates expression of p53 isoforms. Our results suggest that there is interplay between miRNAs and PTB at the 3′UTR under normal and stress conditions like DNA damage. Interestingly, PTB showed some overlapping binding regions in the p53 3′UTR with miR-1285. In fact, knockdown of miR-1285 as well as expression of p53 3′UTR with mutated miR-1285 binding sites resulted in enhanced association of PTB with the 3′UTR, which provides mechanistic insights of this interplay. Taken together, the results provide a plausible molecular basis of how the interplay between miRNAs and the PTB protein at the 3′UTR can play pivotal role in fine tuning the expression of the two p53 isoforms. PMID:28973454

The Glycogen Synthase Kinase 3α and β Isoforms Differentially Regulates Interleukin-12p40 Expression in Endothelial Cells Stimulated with Peptidoglycan from Staphylococcus aureus.

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Ricarda Cortés-Vieyra

Full Text Available Glycogen synthase kinase 3 (GSK3 is a constitutively active regulatory enzyme that is important in cancer, diabetes, and cardiovascular, neurodegenerative, and psychiatric diseases. While GSK3α is usually important in neurodegenerative and psychiatric diseases GSK3β is fundamental in the inflammatory response caused by bacterial components. Peptidoglycan (PGN, one of the most abundant cell-wall structures of Gram-positive bacteria, is an important inducer of inflammation. To evaluate whether inhibition of GSK3α and GSK3β activity in bovine endothelial cells (BEC regulates the expression of the pro-inflammatory cytokine IL-12p40, we treated BEC with SDS-purified PGN from Staphylococcus aureus. We found that PGN triggered a TLR2/PI3K/Akt-dependent phosphorylation of GSK3α at Ser21, GSK3β at Ser9, and NF-κB p65 subunit (p65 at Ser536, and the phosphorylation of GSK3α was consistently higher than that of GSK3β. The expression of IL-12p40 was inhibited in BEC stimulated with PGN and pre-treated with a specific neutralizing anti-TLR2 antibody that targets the extracellular domain of TLR2 or by the addition of Akt-i IV (an Akt inhibitor. Inhibition of GSK3α and GSK3β with LiCl or SB216763 induced an increase in IL-12p40 mRNA and protein. The effect of each isoform on IL-12p40 expression was evaluated by siRNA-gene expression silencing of GSK3α and GSK3β. GSK3α gene silencing resulted in a marked increase in IL-12p40 mRNA and protein while GSK3β gene silencing had the opposite effect on IL-12p40 expression. These results indicate that the TLR2/PI3K/Akt-dependent inhibition of GSK3α activity also plays an important role in the inflammatory response caused by stimulation of BEC with PGN from S. aureus.

Momentum and angular characteristics of diffractional and nondiffractional π-p interactions at 40 GeV/c

International Nuclear Information System (INIS)

Grishin, V.G.; Kerachev, P.P.; Tsivtsivadze, Eh.T.

1976-01-01

The πsup(+-)-meson and γ-quantum distribution on the longitudinal rapidities and transversal momenta in diffractional and nondiffractional interactions at p=40 GeV/c are presented. The topological cross sections and the dependence of the average multiplicity of π 0 -mesons on the number of secondary charged particles in the diffraction interactions have been determined

Identification and Characterization of CYP9A40 from the Tobacco Cutworm Moth (Spodoptera litura), a Cytochrome P450 Gene Induced by Plant Allelochemicals and Insecticides

Science.gov (United States)

Wang, Rui-Long; Staehelin, Christian; Xia, Qing-Qing; Su, Yi-Juan; Zeng, Ren-Sen

2015-01-01

Cytochrome P450 monooxygenases (P450s) of insects play crucial roles in the metabolism of endogenous and dietary compounds. Tobacco cutworm moth (Spodoptera litura), an important agricultural pest, causes severe yield losses in many crops. In this study, we identified CYP9A40, a novel P450 gene of S. litura, and investigated its expression profile and potential role in detoxification of plant allelochemicals and insecticides. The cDNA contains an open reading frame encoding 529 amino acid residues. CYP9A40 transcripts were found to be accumulated during various development stages of S. litura and were highest in fifth and sixth instar larvae. CYP9A40 was mainly expressed in the midgut and fat body. Larval consumption of xenobiotics, namely plant allelochemicals (quercetin and cinnamic acid) and insecticides (deltamethrin and methoxyfenozide) induced accumulation of CYP9A40 transcripts in the midgut and fat body. Injection of dsCYP9A40 (silencing of CYP9A40 by RNA interference) significantly increased the susceptibility of S. litura larvae to the tested plant allelochemicals and insecticides. These results indicate that CYP9A40 expression in S. litura is related to consumption of xenobiotics and suggest that CYP9A40 is involved in detoxification of these compounds. PMID:26393579

Reaction π-p → eta'n in the 15-40 GeV/c momentum range

International Nuclear Information System (INIS)

Apel, W.D.; Augenstein, K.H.; Krueger, M.; Mueller, H.; Schinzel, D.; Schneider, H.; Sigurdsson, G.; Bertolucci, E.; Mannelli, I.; Pierazzini, G.M.; Quaglia, M.; Scribano, A.; Sergiampietri, F.; Vincelli, M.L.; Donskov, S.V.; Inyakin, A.V.; Johnson, R.; Kachanov, V.A.; Krasnokutsky, R.N.; Lednev, A.A.; Mikhailov, Yu.V.; Prokoshkin, Yu.D.; Shuvalov, R.S.; Kittenberger, V.; Leder, G.; Steuer, M.

1979-01-01

Measurements were made of the cross section of the reactions π - p → eta'(958)n, eta' → 2γ at momenta of 15, 20, 25, 30, and 40 GeV/c. The experiment was carried out on the IHEP 70 GeV accelerator using the 648 channel hodoscope spectrometer NICE for γ-ray detection. A total of 6000 eta' mesons were recorded. A sharp drop is seen in the differential cross section for t → 0. The dependences of the differential cross sections π - p → eta'n and π-p → etan on t are identical. On the basis of the ratio of the cross sections for these reactions at t = 0, i.e. R(eta'/n)sub(t=0) = 0.55 +- 0.06, the singlet-octet mixing angle for pseudoscalar mesons was determined to be β = -(18.2 +- 1.4) 0 . (Auth.)

Vector analyzing power and cross section for the reactions 40Ar(d(pol),p)41Ar and 40Ar(d(pol),t)39Ar

International Nuclear Information System (INIS)

Sen, S.; Darden, S.E.; Yoh, W.A.; Berners, E.D.

1975-01-01

Angular distributions of vector analyzing power and cross section for the reactions 40 Ar(d,p) 41 Ar and 40 Ar(d,t) 39 Ar have been measured at an incident deuteron energy of 14.83 MeV. The bound-state data in the (d,p) reaction and the (d,t) data are compared to DWBA calculations. The data for the neutron-unbound states are analyzed in the framework of the DWBA using (i) a form factor for weakly bound neutron and (ii) a resonance form factor, following the approach of Vincent and Fortune. The j-dependence of the (d,p) vector analyzing power permits definite spin-parity assignments to be made for 19 neutron-bound and 4 neutron unbound states in 41 Ar with excitation energy up to 6.57 MeV. Tentative Jsup(π) assignments have been made for 3 states. The l-value for the 5.62 MeV states has been determined. Data for the observed unbound states are found to be equally well reproduced by the type (i) calculations as by the type (ii) calculations. The (d,t) vector cnalyzing power data show definite J-dependence although not as strongly as in the (d,p) reaction. For relatively weakly excited states a pre-knowledge of the l-value of the transition is desirable for an unambiguous spin assignment. In general, the J-dependence in the (d,t) vector analyzing power can be utilized for definitive Jsup(π) assignments. On this basis, Jsub(π) values have been assigned for seven states in 39 Ar with excitation energies up to 4.47MeV excitation. Possible spin values for three other states are suggested. Spectroscopic factors fo the states in 41 Ar and 39 Ar have been extracted and are in fair agreement with those obtained by other workers. (Auth.)

Mössbauer spectroscopy study of 60P2O5-40Fe2O3 glass crystallization

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Stoch Paweł

2015-03-01

Full Text Available 60P2O5-40Fe2O3 glass was synthesized and 57Fe Mössbauer spectroscopy study was presented. The main goal of the research was to investigate structural changes of local environment of iron ions during gradual crystallization of the glass. It was observed that some changes were evidenced at temperature of heat treatment higher than 400°C, above which content of tetrahedrally coordinated Fe3+ was increased in cost of octahedral sites. This led to formation of areas of nucleation of α-FePO4. Crystallization of α-Fe3(P2O72 and Fe2P2O7 was also observed.

p53 levels, cell cycle kinetics and radiosensitivity in two SV40 transformed Wi38VA13 fibroblast strains

International Nuclear Information System (INIS)

Werner, F.; Zoelzer, F.; Streffer, C.

2001-01-01

Background: The tumor suppressor protein p53 which can mediate an ionizing radiation-induced G 1 arrest in mammalian cells, forms complexes with SV40 large T antigen (l-T-Ag). We have analyzed the p53 levels, the capability to undergo a G 1 arrest and the radiosensitivity of two SV40 transformed fibroblast strains differing in their large T antigen expression. Material and Methods: One of the two strains (VA13F) is the commercially available form of Wi38VA13, the other (VA13E) arose spontaneously from the original one in our laboratory. Their p53 levels were measured by means of flow cytometry (FCM) and Western blot (WB) with two p53 antibodies (Ab-3, clone PAb240; Ab-6, clone DO-1; both Oncogene Science). Cell cycle distributions were determined flow cytometrically after BrdU labeling at regular time intervals after exposure to 250 kV X-rays. Radiosensitivity was assessed in a clonogenicity assay. Results: The p53 levels of the two strains corresponded to their large T antigen expression, presumably due to complex formation between the two proteins. The strain with a high p53 level did not show a G 1 arrest and had a relatively high radiosensitivity, whereas the strain with a low p53 level showed a significant G 1 arrest and a lower radiosensitivity. Conclusion: These results suggest that 1. complex formation between the large T antigen and p53 reduces the latter's functionality; 2. in these two strains the G 1 arrest is one of the factors determining radiosensitivity. (orig.) [de

p53-stabilizing Agent CP-31398 Prevents Growth and Invasion of Urothelial Cancer of the Bladder in Transgenic UPII-SV40T Mice

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Venkateshwar Madka

2013-08-01

Full Text Available The high prevalence of bladder cancer and its recurrence make it an important target for chemoprevention. About half of invasive urothelial tumors have mutations in p53. We determined the chemopreventive efficacy of a p53-stabilizing agent, CP-31398, in a transgenic UPII-SV40T mouse model of bladder transitional cell carcinoma (TCC that strongly resembles human TCC. After genotyping, six-week-old UPII-SV40T mice (n = 30/group were fed control (AIN-76A or experimental diets containing 150 or 300 ppm of CP-31398 for 34 weeks. Progression of bladder cancer growth was monitored by magnetic resonance imaging. At 40 weeks of age, all mice were killed; urinary bladders were collected to determine weights, tumor incidence, and histopathology. There was a significant increase in bladder weights of transgenic versus wild-type mice (male: 140.2 mg vs 27.3 mg, P < .0001; female: 34.2 mg vs 14.8 mg, P < .0001. A significant decrease in the bladder tumor weights (by 68.6–80.2%, P < .0001 in males and by 36.9–55.3%, P < .0001 in females was observed in CP-31398-treated mice. Invasive papillary TCC incidence was 100% in transgenic mice fed control diet. Both male and female mice exposed to CP-31398 showed inhibition of invasive TCC. CP-31398 (300 ppm completely blocked invasion in female mice. Molecular analysis of the bladder tumors showed an increase in apoptosis markers (p53, p21, Bax, and Annexin V with a decrease in vascular endothelial growth factor in transgenic mice fed CP-31398. These results suggest that p53-modulating agents can serve as potential chemopreventive agents for bladder TCC.

YKL-40-Induced Inhibition of miR-590-3p Promotes Interleukin-18 Expression and Angiogenesis of Endothelial Progenitor Cells

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Te-Mao Li

2017-04-01

Full Text Available YKL-40, also known as human cartilage glycoprotein-39 or chitinase-3-like-1, is a pro-inflammatory protein that is highly expressed in rheumatoid arthritis (RA patients. Angiogenesis is a critical step in the pathogenesis of RA, promoting the infiltration of inflammatory cells into joints and providing oxygen and nutrients to RA pannus. In this study, we examined the effects of YKL-40 in the production of the pro-inflammatory cytokine interleukin-18 (IL-18, and the stimulation of angiogenesis and accumulation of osteoblasts. We observed that YKL-40 induces IL-18 production in osteoblasts and thereby stimulates angiogenesis of endothelial progenitor cells (EPCs. We found that this process occurs through the suppression of miR-590-3p via the focal adhesion kinase (FAK/PI3K/Akt signaling pathway. YKL-40 inhibition reduced angiogenesis in in vivo models of angiogenesis: the chick embryo chorioallantoic membrane (CAM and Matrigel plug models. We report that YKL-40 stimulates IL-18 expression in osteoblasts and facilitates EPC angiogenesis.

CD40 in Retinal Müller Cells Induces P2X7-Dependent Cytokine Expression in Macrophages/Microglia in Diabetic Mice and Development of Early Experimental Diabetic Retinopathy.

Science.gov (United States)

Portillo, Jose-Andres C; Lopez Corcino, Yalitza; Miao, Yanling; Tang, Jie; Sheibani, Nader; Kern, Timothy S; Dubyak, George R; Subauste, Carlos S

2017-02-01

Müller cells and macrophages/microglia are likely important for the development of diabetic retinopathy; however, the interplay between these cells in this disease is not well understood. An inflammatory process is linked to the onset of experimental diabetic retinopathy. CD40 deficiency impairs this process and prevents diabetic retinopathy. Using mice with CD40 expression restricted to Müller cells, we identified a mechanism by which Müller cells trigger proinflammatory cytokine expression in myeloid cells. During diabetes, mice with CD40 expressed in Müller cells upregulated retinal tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β), intracellular adhesion molecule 1 (ICAM-1), and nitric oxide synthase (NOS2), developed leukostasis and capillary degeneration. However, CD40 did not cause TNF-α or IL-1β secretion in Müller cells. TNF-α was not detected in Müller cells from diabetic mice with CD40 + Müller cells. Rather, TNF-α was upregulated in macrophages/microglia. CD40 ligation in Müller cells triggered phospholipase C-dependent ATP release that caused P2X 7 -dependent production of TNF-α and IL-1β by macrophages. P2X 7 -/- mice and mice treated with a P2X 7 inhibitor were protected from diabetes-induced TNF-α, IL-1β, ICAM-1, and NOS2 upregulation. Our studies indicate that CD40 in Müller cells is sufficient to upregulate retinal inflammatory markers and appears to promote experimental diabetic retinopathy and that Müller cells orchestrate inflammatory responses in myeloid cells through a CD40-ATP-P2X 7 pathway. © 2017 by the American Diabetes Association.

ANALYSIS OF SPIN-ORBIT ALIGNMENT IN THE WASP-32, WASP-38, AND HAT-P-27/WASP-40 SYSTEMS

Energy Technology Data Exchange (ETDEWEB)

Brown, D. J. A.; Collier Cameron, A.; Enoch, B.; Miller, G. R. M. [SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, Fife KY16 9SS (United Kingdom); Diaz, R. F. [LAM (Laboratoire d' Astrophysique de Marseille), Aix Marseille Universite, CNRS, UMR 7326, F-13388 Marseille (France); Doyle, A. P.; Smalley, B.; Anderson, D. R.; Hellier, C.; Maxted, P. F. L. [Astrophysics Group, School of Physical and Geographical Sciences, Lennard-Jones Building, Keele University, Staffordshire ST5 5BG (United Kingdom); Gillon, M. [Institut d' Astrophysique et de Geophysique, Universite de Liege, Allee du 6 Aout, 17 (Bat. B5C) Sart Tilman, B-4000 Liege (Belgium); Lendl, M.; Triaud, A. H. M. J.; Queloz, D. [Observatoire Astronomique de l' Universite de Geneve, 51 Chemin des Maillettes, CH-1290 Sauverny (Switzerland); Pollacco, D. [Astrophysics Research Centre, School of Mathematics and Physics, Queen' s University, University Road, Belfast BT7 1NN (United Kingdom); Boisse, I. [Centro de Astrofisica, Universidade do Porto, Rua das Estrelas, 4150-762 Porto (Portugal); Hebrard, G., E-mail: djab@st-andrews.ac.uk [Institut dAstrophysique de Paris, UMR7095 CNRS, Universite Pierre and Marie Curie, 98bis boulevard Arago, F-75014 Paris (France)

2012-12-01

We present measurements of the spin-orbit alignment angle, {lambda}, for the hot Jupiter systems WASP-32, WASP-38, and HAT-P-27/WASP-40, based on data obtained using the HARPS spectrograph. We analyze the Rossiter-McLaughlin effect for all three systems and also carry out Doppler tomography for WASP-32 and WASP-38. We find that WASP-32 (T {sub eff} = 6140{sup +90} {sub -100} K) is aligned, with an alignment angle of {lambda} = 10.{sup 0}5{sup +6.4} {sub -6.5} obtained through tomography, and that WASP-38 (T {sub eff} = 6180{sup +40} {sub -60} K) is also aligned, with tomographic analysis yielding {lambda} = 7.{sup 0}5{sup +4.7} {sub -6.1}. The latter result provides an order-of-magnitude improvement in the uncertainty in {lambda} compared to the previous analysis of Simpson et al. We are only able to loosely constrain the angle for HAT-P-27/WASP-40 (T{sub eff} = 5190{sup +160} {sub -170} K) to {lambda} = 24.{sup 0}2{sup +76.0}{sub -44.5}, owing to the poor signal-to-noise ratio of our data. We consider this result a non-detection under a slightly updated version of the alignment test of Brown et al. We place our results in the context of the full sample of spin-orbit alignment measurements, finding that they provide further support for previously established trends.

The study of p-Be collisions at 40 GeV/c z experiment NA57 at SPS accelerator at CERN

International Nuclear Information System (INIS)

Bombara, M.

2005-01-01

In this work the p-Be collisions at 40 GeV/c in NA57 experiment at CERN SPS were analysed. These collisions are expected to constitute a reference sample for a study of the strangeness production enhancement in Pb-Pb collisions. We concentrated on the analysis of strange particles Κ 0 , Λ and Λ'bar'. The particle signals were extracted by reconstructing their weak decays into nal states containing only charged particles: K 0 S → π + π - , Λ → pπ - and Λ'-' → p'-'π + using geometric and kinematic constraints. The data were corrected for geometrical acceptance and detector and reconstruction efficiencies on an event-by-event basis. For each particle species we defined the acceptance window using a Monte Carlo simulation of the apparatus. The double-differential (y,m T ) distribution for each particle species has been parametrized assuming the rapidity distribution to be at and the transverse momenta distribution to be exponential within our acceptance region. We extrapolated the yield measured in the selected acceptance window to a common phase space window covering full p T and one unit of rapidity centred at mid-rapidity. By comparison of yields in p-Be collisions and yields in Pb-Pb collisions we obtained the enhancements of strange production. The investigation of rapidity spectra shows non at behaviour for K 0 . A significant enhancement of strangeness production in Pb-Pb collisions with respect to p-Be collisions is observed in the 40 GeV/c data. (author)

Cerebrospinal fluid IL-12p40, CXCL13 and IL-8 as a combinatorial biomarker of active intrathecal inflammation.

Directory of Open Access Journals (Sweden)

Bibiana Bielekova

Full Text Available Diagnosis and management of the neuroinflammatory diseases of the central nervous system (CNS are hindered by the lack of reliable biomarkers of active intrathecal inflammation. We hypothesized that measuring several putative inflammatory biomarkers simultaneously will augment specificity and sensitivity of the biomarker to the clinically useful range. Based on our pilot experiment in which we measured 18 inflammatory biomarkers in 10-fold concentrated cerebrospinal fluid (CSF derived from 16 untreated patients with highly active multiple sclerosis (MS we selected a combination of three CSF biomarkers, IL-12p40, CXCL13 and IL-8, for further validation.Concentrations of IL-12p40, CXCL13 and IL-8 were determined in a blinded fashion in CSF samples from an initial cohort (n = 72 and a confirmatory cohort (n = 167 of prospectively collected, untreated subjects presenting for a diagnostic work-up of possible neuroimmunological disorder. Diagnostic conclusion was based on a thorough clinical workup, which included laboratory assessment of the blood and CSF, neuroimaging and longitudinal follow-up. Receiver operating characteristic (ROC curve analysis in conjunction with principal component analysis (PCA, which was used to combine information from all three biomarkers, assessed the diagnostic value of measured biomarkers.Each of the three biomarkers was significantly increased in MS and other inflammatory neurological disease (OIND in comparison to non-inflammatory neurological disorder patients (NIND at least in one cohort. However, considering all three biomarkers together improved accuracy of predicting the presence of intrathecal inflammation to the consistently good to excellent range (area under the ROC curve = 0.868-0.924.Future clinical studies will determine if a combinatorial biomarker consisting of CSF IL-12p40, CXCL13 and IL-8 provides utility in determining the presence of active intrathecal inflammation in diagnostically

Structural behavior of Pd40Cu30Ni10P20 bulk metallic glass below and above the glass transition

DEFF Research Database (Denmark)

Mattern, N.; Hermann, H.; Roth, S.

2003-01-01

The thermal behavior of the structure of Pd40Cu30Ni10P20 bulk metallic glass has been investigated in situ through the glass transition by means of high-temperature x-ray synchrotron diffraction. The dependence of the x-ray structure factor S(q) of the Pd40Cu30Ni10P20 glass on temperature follows...... the Debye theory up to the glass transition with a Debye temperature theta=296 K. Above the glass transition temperature T-g, the temperature dependence of S(q) is altered, pointing to a continuous development of structural changes in the liquid with temperature. The atomic pair correlation functions g......(r) indicate changes in short-range-order parameters of the first and the second neighborhood with temperature. The temperature dependence of structural parameters is different in glass and in supercooled liquid, with a continuous behavior through the glass transition. The nearest-neighbor distance decreases...

Nuclear Data Sheets for A=40

Energy Technology Data Exchange (ETDEWEB)

Chen, Jun

2017-02-15

The experimental nuclear structure data and decay data are evaluated for the known nuclides of mass 40 (Mg, Al, Si, P, S, Cl, Ar, K, Ca, Sc, Ti). Detailed evaluated nuclear structure information is presented with the best values recommended for level energies, half-lives, γ-ray energies and intensities, decay properties (energies, intensities and placement of radiations), and other spectroscopic data. The {sup 40}Ca and {sup 40}K nuclides remain as the most extensively studied from many different reactions and decays; no excited states are known in {sup 40}Mg, {sup 40}Al, {sup 40}P and {sup 40}Ti. This work supersedes the earlier full evaluation of A=40 by J. Cameron and B. Singh (2004Ca38).

Caracterización molecular de las proteínas p47phox y p40phox del sistema NADPH oxidasa mediante mutagénesis sitio dirigida

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Juan Alvaro López

2000-02-01

Full Text Available <p class="MsoNormal">Los neutrófilos responden a diferentes estímulos con un fenómeno conocido como “explosión respiratoria” la cual depende del sistema NADPH oxidasa. La importancia de este sistema para la actividad microbicida de las células fagocíticas es evidente en pacientes con Enfermedad Granulomatosa Crónica (EGC, los cuales sufren un incremento en la susceptibilidad a infecciones por hongos y bacterias debido a una deficiencia en la producción de agentes oxidantes por los fagocitos. El sistema NADPH oxidasa está formado por seis componentes esenciales: p40phox, p47phox, p67phox, p22phox y gp91phox. Una sexta proteína implicada en la activación del sistema es la proteína p21rac, de la familia de las GTPasas relacionadas con Ras. El centro funcional de este sistema se encuentra en el flavocitocromo b558, compuesto por las subunidades gp91phox y p22phox ubicadas en la membrana de las vesículas secretorias y gránulos específicos, las cuales aceptan electrones del NADPH citosólico y lo donan al oxígeno molecular que se encuentra en la vacuola fagocítica. p> <p class

Coincidence (e,e'p) Scattering on 40Ar and 48Ti to Aid Precision Neutrino Oscillation Experiments

Science.gov (United States)

Abrams, Dan; E12-14-012 Collaboration

2017-09-01

Neutrino oscillations are an active area of research, with experiments such as DUNE (Deep Underground Neutrino Experiment). DUNE will make use of large liquid argon detectors to perform a precision measurement of the CP violating phase. Hence, an understanding of the argon nuclear ground state and its response to (anti-)neutrino interactions is of paramount importance. Information about the nuclear ground state is encapsulated in the spectral function, S (k , E) , the joint probability of removing a nucleon of momentum k = |k | from the ground state leaving the residual (A-1) system with excitation energy E. E12-14-012 at Jefferson Lab ran in early 2017 and has measured the argon spectral function through coincidence (e ,e' p) scattering on 40Ar and 48Ti. The results of E12-14-012 are important to both the neutrino and nuclear physics communities. A direct measurement of the coincidence (e ,e' p) cross section from 40Ar and 48Ti will provide valuable information about the argon nucleus, as well as the experimental input necessary to constrain theoretical models used to calculate S (k , E) , paving the way for reliable estimates of the neutrino cross sections. Data from E12-14-012 is currently being analyzed at UVA and Va. Tech. Supported in part by the Department of Energy Grant No: DE-FG02-96ER40950.

Mycobacterium tuberculosis PPE44 (Rv2770c) is involved in response to multiple stresses and promotes the macrophage expression of IL-12 p40 and IL-6 via the p38, ERK, and NF-κB signaling axis.

Science.gov (United States)

Yu, Zhaoxiao; Zhang, Chenhui; Zhou, Mingliang; Li, Qiming; Li, Hui; Duan, Wei; Li, Xue; Feng, Yonghong; Xie, Jianping

2017-09-01

Tuberculosis (TB), caused by Mycobacterium tuberculosis, remains a formidable threat to global public health. The successful intracellular persistence of M. tuberculosis significantly contributes to the intractability of tuberculosis. Proline-glutamic acid (PE) and proline-proline-glutamic acid (PPE) are mycobacterial exclusive protein families that widely reported to be involved in the bacterial virulence, physiology and interaction with host. Rv2770c (PPE44), a predicted virulence factor, was up-regulated upon the infected guinea pig lungs. To investigate the role of Rv2770c, we heterologously expressed the PPE44 in the nonpathogenic fast-growing M. smegmatis strain. Subcellular location analysis demonstrated that Rv2770c is a cell wall associated protein, suggestive of a potential candidate involved in host-pathogen interaction. The Rv2770c can enhance M. smegmatis survival within macrophages and under stresses such as H 2 O 2 , SDS, diamide exposure, and low pH condition. M. smegmatis expressing Rv2770c is more virulent as testified by the increased death of macrophages and the increased expression of interlukin-6 (IL-6) and interlukin-12p40 (IL-12p40). Moreover, Rv2770c altered the secretion of IL-6 and IL-12p40 of macrophages via NF-κB, ERK1/2 and p38 MAPK axis. Taken together, this study implicated that Rv2770c was a virulent factor actively engaged in the interaction with host macrophage. Copyright © 2017. Published by Elsevier B.V.

Increased production of IL-4 and IL-12p40 from bronchoalveolar lavage cells are biomarkers of Mycobacterium tuberculosis in the sputum.

Directory of Open Access Journals (Sweden)

Anna Nolan

Full Text Available Tuberculosis (TB causes 1.45 million deaths annually world wide, the majority of which occur in the developing world. Active TB disease represents immune failure to control latent infection from airborne spread. Acid-fast bacillus (AFB seen on sputum smear is a biomarker for contagiousness.We enrolled 73 tuberculosis patients with extensive infiltrates into a research study using bronchoalveolar lavage (BAL to sample lung immune cells and assay BAL cell cytokine production. All patients had sputum culture demonstrating Mycobacterium tuberculosis and 59/73 (81% had AFB identified by microscopy of the sputum. Compared with smear negative patients, smear positive patients at presentation had a higher proportion with smoking history, a higher proportion with temperature >38.5(0 C, higher BAL cells/ml, lower percent lymphocytes in BAL, higher IL-4 and IL-12p40 in BAL cell supernatants. There was no correlation between AFB smear and other BAL or serum cytokines. Increasing IL-4 was associated with BAL PMN and negatively associated with BAL lymphocytes. Each 10-fold increase in BAL IL-4 and IL-12p40 increased the odds of AFB smear positivity by 7.4 and 2.2-fold, respectively, in a multi-variable logistic model.Increasing IL-4 and IL-12p40 production by BAL cells are biomarkers for AFB in sputum of patients who present with radiographically advanced TB. They likely reflect less effective immune control of pathways for controlling TB, leading to patients with increased infectiousness.

Two neutron transfer form factor for the reaction 42Ca(p,t)40Ca

International Nuclear Information System (INIS)

Meyer, R.H.

1978-01-01

In an attempt to better interpret experimental data concerning the two-neutron pickup process 42 Ca(p,t) 40 Ca, a detailed study of the form factors associated with the reaction is carried out. A set of coupled integro-differential equations describing these form factors is derived, starting from a microscopic, model-independent Hamiltonian. These equations allow contributions to the form factors from hole terms as well as from the particle and so-called ''continuum'' states, which were previously studied. An approximate solution of the form factor equations is obtained by neglecting the coupling terms and expressing the form factor in terms of a set of Sturmian states. Form factors for the transition to the 40 Ca ground state (O 1 + ) are calculated using various sets of Sturmian states. The inclusion of hole states is found to have a major effect upon both the shape of the form factor and the size of the related cross section. Finally, a comparison is made between the O 1 + form factors calculated using Sturmian states and a O 1 + form factor obtained using Sturmian states and a O 1 + form factor obtained using the coexistence model. It is found that a form factor based on Sturmian particle and hole states is very similar to the form factor obtained from the coexistence model calculation

Structures and electron affinity of XO30,-, XOF40,- and XO2F20,- (X = P, As, Sb, Bi): a theoretical study of novel superhalogen formulae and exceptions of superhalogen formulae

Science.gov (United States)

Yang, Yi-Fan; Cui, Zhong-Hua; Ding, Yi-Hong

2015-03-01

Most superhalogen species are in the form of oxides or halides. To enrich the family of superhalogen species, herein, we investigated the structures and electron affinity (EA) values of higher group 15 elements (X = P, As, Sb, Bi) oxyfluoride species XO30,-, XOF40,- and XO2F20,-, at the CCSD(T)/aug-cc-pVTZ-pp & aug-cc-pVTZ //B3LYP/aug-cc-pVTZ-pp & aug-cc-pVTZ levels (aug-cc-pVTZ-pp for X = Sb and Bi). Some oxyfluoride species, i.e., PO2F20,-, AsO2F20,-, SbO2F20,-, POF40,-, AsOF40,-, SbOF40,- and BiOF40,-, were found to possess higher EA (VDE: 5.0-6.2 eV; ADE: 4.5-5.5 eV) than halogens (F: 3.4 eV; Cl: 3.6 eV). Thus, we recommended that the oxyfluorides in the form of XO2F20,- and XOF40,- should be considered as potential superhalogens, which have not been considered previously. Surprisingly, we showed that BiO3 and BiO2F2, in superhalogen formulae, possess a high vertical detachment energy (VDE) yet a low adiabatic detachment energy (ADE). This is in marked contrast to the previously reported superhalogens, which generally contain both the high VDE and high ADE values. It is the first report about exceptions of superhalogen formulae. These findings revealed that for the analogous main-group compounds with the same structural formula, the difference in the metallic property of the core element could lead to the significant difference in the ground structures of either the anionic or neutral structures, which would result in the much differed superhalogen features.

Measurements of π±, K±, p and p spectra in proton-proton interactions at 20, 31, 40, 80 and 158 GeV/c with the NA61/SHINE spectrometer at the CERN SPS

International Nuclear Information System (INIS)

Aduszkiewicz, A.; Dominik, W.; Kuich, M.; Matulewicz, T.; Podlaski, P.; Posiadala, M.; Walewski, M.; Ali, Y.; Brzychczyk, J.; Larsen, D.; Planeta, R.; Richter-Was, E.; Staszel, P.; Wyszynski, O.; Andronov, E.; Feofilov, G.A.; Igolkin, S.; Kondratiev, V.P.; Kovalenko, V.; Merzlaya, A.; Seryakov, A.; Vechernin, V.V.; Vinogradov, L.; Anticic, T.; Kadija, K.; Susa, T.; Baatar, B.; Bunyatov, S.A.; Kireyeu, V.A.; Kolesnikov, V.I.; Krasnoperov, A.; Lyubushkin, V.V.; Malakhov, A.I.; Matveev, V.; Melkumov, G.L.; Tereshchenko, V.; Baszczyk, M.; Dorosz, P.; Kucewicz, W.; Mik, L.; Bhosale, S.; Davis, N.; Kielbowicz, M.; Marcinek, A.; Ozvenchuk, V.; Rybicki, A.; Blondel, A.; Bravar, A.; Damyanova, A.; Haesler, A.; Korzenev, A.; Ravonel, M.; Bogomilov, M.; Kolev, D.; Tsenov, R.; Brandin, A.; Selyuzhenkov, I.; Strikhanov, M.; Taranenko, A.; Busygina, O.; Golubeva, M.; Guber, F.; Ivashkin, A.; Kurepin, A.; Sadovsky, A.; Cherif, H.; Deveaux, M.; Klochkov, V.; Koziel, M.; Renfordt, R.; Snoch, A.; Stroebele, H.; Toia, A.; Cirkovic, M.; Knezevic, N.; Manic, D.; Puzovic, J.; Czopowicz, T.; Grebieszkow, K.; Mackowiak-Pawlowska, M.; Maksiak, B.; Slodkowski, M.; Tefelska, A.; Tefelski, D.; Dembinski, H.; Engel, R.; Herve, A.E.; Mathes, H.J.; Roth, M.; Szuba, M.; Ulrich, R.; Unger, M.; Veberic, D.; Dumarchez, J.; Ereditato, A.; Francois, C.; Pistillo, C.; Wilkinson, C.; Fodor, Z.; Garibov, A.; Gazdzicki, M.; Hylen, J.; Lundberg, B.; Marchionni, A.; Rameika, R.; Zwaska, R.; Johnson, S.R.; Marino, A.D.; Nagai, Y.; Rumberger, B.T.; Zimmerman, E.D.; Kaptur, E.; Kowalski, S.; Lysakowski, B.; Pulawski, S.; Schmidt, K.; Kowalik, K.; Rondio, E.; Stepaniak, J.; Laszlo, A.; Marton, K.; Vesztergombi, G.; Lewicki, M.; Naskret, M.; Turko, L.; Messerly, B.; Paolone, V.; Wickremasinghe, A.; Mills, G.B.; Morozov, S.; Petukhov, O.; Mrowczynski, S.; Rybczynski, M.; Seyboth, P.; Stefanek, G.; Wlodarczyk, Z.; Wojtaszek-Szwarc, A.; Pavin, M.; Popov, B.A.; Rauch, W.; Roehrich, D.; Rustamov, A.; Zambelli, L.

2017-01-01

Measurements of inclusive spectra and mean multiplicities of π ± , K ± , p and p produced in inelastic p + p interactions at incident projectile momenta of 20, 31, 40, 80 and 158 GeV/c (√(s) = 6.3, 7.7, 8.8, 12.3 and 17.3 GeV, respectively) were performed at the CERN Super Proton Synchrotron using the large acceptance NA61/SHINE hadron spectrometer. Spectra are presented as function of rapidity and transverse momentum and are compared to predictions of current models. The measurements serve as the baseline in the NA61/SHINE study of the properties of the onset of deconfinement and search for the critical point of strongly interacting matter. (orig.)

Elastic properties of Pd40Cu30Ni10P20 bulk glass in supercooled liquid region

DEFF Research Database (Denmark)

Nishiyama, N.; Inoue, A.; Jiang, Jianzhong

2001-01-01

In situ ultrasonic measurements for the Pd40Cu30Ni10P20 bulk glass in three states: Glassy solid, supercooled liquid, and crystalline, have been performed. It is found that velocities of both longitudinal and transverse waves and elastic moduli (shear modulus, bulk modulus, Young's modulus......, and Lame parameter), together with Debye temperature, gradually decrease with increasing temperature through the glass transition temperature as the Poisson's ratio increases. The behavior of the velocity of transverse wave vs. temperature in the supercooled liquid region could be explained by viscosity...

Eh-pH diagrams for elements from Z = 40 to Z = 52: application to the Oklo natural reactor, Gabon

Energy Technology Data Exchange (ETDEWEB)

Brookins, D G [New Mexico Univ., Albuquerque (USA). Dept. of Geology

1978-11-01

Eh-pH diagrams for elements from Z = 40 (Zr) to Z = 52 (Te) have been constructed in order to comment on migration/retention of these elements at Oklo. Although data for fissiogenic amounts of some of these elements are lacking, where such data are available to agreement between predicted migration/retention based on the Eh-pH diagrams and actual measurement is excellent. Based on Eh-pH diagrams, migration (to what degree is uncertain) of Mo and Cd is predicted whereas retention of Y, Zr, Tc, Ru, Rh, Pd, Ag and Te is also predicted. An earlier report of Frejacques et al. of Tc migration is in disagreement with Eh-pH prediction, and recent (unpublished) data argue for Tc retention. In view of the agreement between prediction and observation, the possible migration of Sb and retention of In and Sn is proposed. These data again demonstrate the usefulness of Eh-pH diagrams for the Oklo fossil nuclear reactor but, more important, allow constraints to be placed on repositories for nuclear waste now under consideration.

On the use of polarization modulation in combined interferometry and polarimetry. Corrigendum. 1998 Plasma Phys. Control. Fusion, v. 40 p. 153-161

International Nuclear Information System (INIS)

Segre, S.E.

1998-01-01

Errors in the main text, the appendix and two curves are corrected in this corrigendum to the paper entitled ''On the use of polarization modulation in combined interferometry and polarimetry'', written by S.E. Segre and published in 1998 Plasma Phys. Control. Fusion, v. 40 p. 153-161

Frequency-dependent complex modulus at the glass transition in Pd40Ni10Cu30P20 bulk amorphous alloys

International Nuclear Information System (INIS)

Lee, M.L.; Li, Y.; Feng, Y.P.; Carter, W.C.

2003-01-01

We report frequency-dependent measurements of the dynamic elastic modulus of a Pd 40 Cu 30 Ni 10 P 20 bulk amorphous phase near its glass transition temperature. The storage and loss moduli exhibit a structural relaxation similar to those observed by other characterization techniques. Parameters obtained by fitting to the Vogel-Fulcher-Tamman equation and the Kohlrausch-Williams-Watts model exhibit similar behaviors to those other methods

S P Panda

Indian Academy of Sciences (India)

Home; Journals; Sadhana. S P Panda. Articles written in Sadhana. Volume 40 Issue 1 February 2015 pp 89-106 Electrical and Computer Sciences. Experimental validation of waveform relaxation technique for power system controller testing · S P Panda K A Salunkhe A M Kulkarni · More Details Abstract Fulltext PDF.

YKL-40 tissue expression and plasma levels in patients with ovarian cancer

International Nuclear Information System (INIS)

Høgdall, Estrid VS; Christensen, Lise H; Ringsholt, Merete; Høgdall, Claus K; Christensen, Ib Jarle; Johansen, Julia S; Kjaer, Susanne K; Blaakaer, Jan; Ostenfeld-Møller, Lene; Price, Paul A

2009-01-01

YKL-40 (chitinase-3-like-1) is a member of 'mammalian chitinase-like proteins'. The protein is expressed in many types of cancer cells and the highest plasma YKL-40 levels have been found in patients with metastatic disease, short recurrence/progression-free intervals, and short overall survival. The aim of the study was to determine the expression of YKL-40 in tumor tissue and plasma in patients with borderline ovarian tumor or epithelial ovarian cancer (OC), and investigate prognostic value of this marker. YKL-40 protein expression was determined by immunohistochemistry in tissue arrays from 181 borderline tumors and 473 OC. Plasma YKL-40 was determined by ELISA in preoperative samples from 19 patients with borderline tumor and 76 OC patients. YKL-40 protein expression was found in cancer cells, tumor associated macrophages, neutrophils and mast cells. The tumor cell expression was higher in OC than in borderline tumors (p = 0.001), and associated with FIGO stage (p < 0.0001) and histological subtype (p = 0.0009). Positive YKL-40 expression (≥ 5% staining) was not associated with reduced survival. Plasma YKL-40 was also higher in patients with OC than in patients with borderline tumors (p < 0.0001), and it was positively correlated to serum CA-125 (p < 0.0001) and FIGO stage (p = 0.0001). Univariate Cox analysis of plasma YKL-40 showed association with overall survival (p < 0.0001). Multivariate Cox analysis, including plasma YKL-40, serum CA125, FIGO stage, age and radicality after primary surgery as variables, showed that elevated plasma YKL-40 was associated with a shorter survival (HR = 2.13, 95% CI: 1.40–3.25, p = 0.0004). YKL-40 in OC tissue and plasma are related to stage and histology, but only plasma YKL-40 is a prognostic biomarker in patients with OC

Variation in a surface-exposed region of the Mycoplasma pneumoniae P40 protein as a consequence of homologous DNA recombination between RepMP5 elements.

Science.gov (United States)

Spuesens, Emiel B M; van de Kreeke, Nick; Estevão, Silvia; Hoogenboezem, Theo; Sluijter, Marcel; Hartwig, Nico G; van Rossum, Annemarie M C; Vink, Cornelis

2011-02-01

Mycoplasma pneumoniae is a human pathogen that causes a range of respiratory tract infections. The first step in infection is adherence of the bacteria to the respiratory epithelium. This step is mediated by a specialized organelle, which contains several proteins (cytadhesins) that have an important function in adherence. Two of these cytadhesins, P40 and P90, represent the proteolytic products from a single 130 kDa protein precursor, which is encoded by the MPN142 gene. Interestingly, MPN142 contains a repetitive DNA element, termed RepMP5, of which homologues are found at seven other loci within the M. pneumoniae genome. It has been hypothesized that these RepMP5 elements, which are similar but not identical in sequence, recombine with their counterpart within MPN142 and thereby provide a source of sequence variation for this gene. As this variation may give rise to amino acid changes within P40 and P90, the recombination between RepMP5 elements may constitute the basis of antigenic variation and, possibly, immune evasion by M. pneumoniae. To investigate the sequence variation of MPN142 in relation to inter-RepMP5 recombination, we determined the sequences of all RepMP5 elements in a collection of 25 strains. The results indicate that: (i) inter-RepMP5 recombination events have occurred in seven of the strains, and (ii) putative RepMP5 recombination events involving MPN142 have induced amino acid changes in a surface-exposed part of the P40 protein in two of the strains. We conclude that recombination between RepMP5 elements is a common phenomenon that may lead to sequence variation of MPN142-encoded proteins.

Muscle strength reduction is related to muscle loss in women over the age of 40. DOI: 10.5007/1980-0037.2011v13n1p36

Directory of Open Access Journals (Sweden)

Fábio Lera Orsatti

2011-12-01

Full Text Available The objective of this study was to compare the influence of age on muscle mass (MM and muscle strength (MS of the upper and lower limbs in women older than 40 years. Fifty-two volunteers were divided into three age groups: 40-49 years (G40, n = 16, 50-59 years (G50, n = 18, and 60-70 years (G60, n = 18. MM was evaluated by bioelectrical impedance and MS by the 1RM test. One-way analysis of variance, Duncan’s test and Pearson’s correlation coefficient were used for statistical analysis. There was a significant difference in MM (p < 0.05 of 10.1% between G40 and G50 and of 7.5% between G50 and G60, corresponding to 16.8% in the two decades (G40/G60. Lower limb MS was 16.4% lower between G40 and G50 and 12.5% between G50 and G60, corresponding to 25.3% in the two decades (p < 0.05. In the upper limbs, there was no significant difference in MS between G40 and G50 (3.3% and 2.5% bench curl or between G50 and G60 (17.5% and 9.6%, corresponding to 20.2% and 12.2% in the two decades, respectively. The sum of MS was corrected by MM (MS/MM and no significant differences were observed between groups. Comparison of MM and MS showed a closer correlation in the lower limbs (r = 0.57 when compared to the upper limbs (r = 0.42 and r = 0.41, respectively. In conclusion, women presented lower MM and MS in the lower limbs as early as in the fifth decade of life. MS/MM does not change in women between 40 and 60 years when compared according to age group. Reduced MM seems to be an important for the lower MS observed in women between 40 and 60 years of age.

Muscle strength reduction is related to muscle loss in women over the age of 40 DOI: 10.5007/1980-0037.2011v13n1p36

Directory of Open Access Journals (Sweden)

Fábio Lera Orsatti

2010-12-01

Full Text Available The objective of this study was to compare the influence of age on muscle mass (MM and muscle strength (MS of the upper and lower limbs in women older than 40 years. Fifty-two volunteers were divided into three age groups: 40-49 years (G40, n = 16, 50-59 years (G50, n = 18, and 60-70 years (G60, n = 18. MM was evaluated by bioelectrical impedance and MS by the 1RM test. One-way analysis of variance, Duncan’s test and Pearson’s correlation coefficient were used for statistical analysis. There was a significant difference in MM (p < 0.05 of 10.1% between G40 and G50 and of 7.5% between G50 and G60, corresponding to 16.8% in the two decades (G40/G60. Lower limb MS was 16.4% lower between G40 and G50 and 12.5% between G50 and G60, corresponding to 25.3% in the two decades (p < 0.05. In the upper limbs, there was no significant difference in MS between G40 and G50 (3.3% and 2.5% bench curl or between G50 and G60 (17.5% and 9.6%, corresponding to 20.2% and 12.2% in the two decades, respectively. The sum of MS was corrected by MM (MS/MM and no significant differences were observed between groups. Comparison of MM and MS showed a closer correlation in the lower limbs (r = 0.57 when compared to the upper limbs (r = 0.42 and r = 0.41, respectively. In conclusion, women presented lower MM and MS in the lower limbs as early as in the fifth decade of life. MS/MM does not change in women between 40 and 60 years when compared according to age group. Reduced MM seems to be an important for the lower MS observed in women between 40 and 60 years of age.

(p,2p) experiments at the University of Maryland cyclotron

International Nuclear Information System (INIS)

Roos, P.G.

1976-11-01

Some of the (p,2p) work which has been carried out at the Maryland Cyclotron is discussed. A brief introduction to the (p,2p) reaction is presented, and the types of experimental techniques utilized in (p,2p) studies are discussed. A brief introduction is given to the various theoretical treatments presently available to analyze (p,2p) reaction data. Secondly, experimental and theoretical studies of (p,2p) on d, 3 He, and 4 He carried out by the Maryland group are presented. Thirdly, (p,2p) results are discussed for 6 Li, 7 Li, and 12 C at 100 MeV. Fourthly, the effects of distortion on the experimental data are considered by presenting theoretical calculations for 12 C and 40 Ca at various bombarding energies

Measurements of π{sup ±}, K{sup ±}, p and p spectra in proton-proton interactions at 20, 31, 40, 80 and 158 GeV/c with the NA61/SHINE spectrometer at the CERN SPS

Energy Technology Data Exchange (ETDEWEB)

Aduszkiewicz, A.; Dominik, W.; Kuich, M.; Matulewicz, T.; Podlaski, P.; Posiadala, M.; Walewski, M. [University of Warsaw, Warsaw (Poland); Ali, Y.; Brzychczyk, J.; Larsen, D.; Planeta, R.; Richter-Was, E.; Staszel, P.; Wyszynski, O. [Jagiellonian University, Krakow (Poland); Andronov, E.; Feofilov, G.A.; Igolkin, S.; Kondratiev, V.P.; Kovalenko, V.; Merzlaya, A.; Seryakov, A.; Vechernin, V.V.; Vinogradov, L. [St. Petersburg State University, St. Petersburg (Russian Federation); Anticic, T.; Kadija, K.; Susa, T. [Ruder Boskovic Institute, Zagreb (Croatia); Baatar, B.; Bunyatov, S.A.; Kireyeu, V.A.; Kolesnikov, V.I.; Krasnoperov, A.; Lyubushkin, V.V.; Malakhov, A.I.; Matveev, V.; Melkumov, G.L.; Tereshchenko, V. [Joint Institute for Nuclear Research, Dubna (Russian Federation); Baszczyk, M.; Dorosz, P.; Kucewicz, W.; Mik, L. [University of Science and Technology, Krakow (Poland); Bhosale, S.; Davis, N.; Kielbowicz, M.; Marcinek, A.; Ozvenchuk, V.; Rybicki, A. [H. Niewodniczanski Institute of Nuclear Physics of the Polish Academy of Sciences, Krakow (Poland); Blondel, A.; Bravar, A.; Damyanova, A.; Haesler, A.; Korzenev, A.; Ravonel, M. [University of Geneva, Geneva (Switzerland); Bogomilov, M.; Kolev, D.; Tsenov, R. [University of Sofia, Faculty of Physics, Sofia (Bulgaria); Brandin, A.; Selyuzhenkov, I.; Strikhanov, M.; Taranenko, A. [National Research Nuclear University (Moscow Engineering Physics Institute), Moscow (Russian Federation); Busygina, O.; Golubeva, M.; Guber, F.; Ivashkin, A.; Kurepin, A.; Sadovsky, A. [Institute for Nuclear Research, Moscow (Russian Federation); Cherif, H.; Deveaux, M.; Klochkov, V.; Koziel, M.; Renfordt, R.; Snoch, A.; Stroebele, H.; Toia, A. [University of Frankfurt, Frankfurt (Germany); Cirkovic, M.; Knezevic, N.; Manic, D.; Puzovic, J. [University of Belgrade, Belgrade (Serbia); Czopowicz, T.; Grebieszkow, K.; Mackowiak-Pawlowska, M.; Maksiak, B.; Slodkowski, M.; Tefelska, A.; Tefelski, D. [Warsaw University of Technology, Warsaw (Poland); Dembinski, H.; Engel, R.; Herve, A.E.; Mathes, H.J.; Roth, M.; Szuba, M.; Ulrich, R.; Unger, M.; Veberic, D. [Karlsruhe Institute of Technology, Karlsruhe (Germany); Dumarchez, J. [University of Paris VI and VII, LPNHE, Paris (France); Ereditato, A.; Francois, C.; Pistillo, C.; Wilkinson, C. [University of Bern, Bern (Switzerland); Fodor, Z. [Wigner Research Centre for Physics of the Hungarian Academy of Sciences, Budapest (Hungary); University of Wroclaw, Wroclaw (Poland); Garibov, A. [National Nuclear Research Center, Baku (Azerbaijan); Gazdzicki, M. [University of Frankfurt, Frankfurt (Germany); Jan Kochanowski University in Kielce, Kielce (Poland); Hylen, J.; Lundberg, B.; Marchionni, A.; Rameika, R.; Zwaska, R. [Fermilab, Batavia (United States); Johnson, S.R.; Marino, A.D.; Nagai, Y.; Rumberger, B.T.; Zimmerman, E.D. [University of Colorado, Boulder (United States); Kaptur, E.; Kowalski, S.; Lysakowski, B.; Pulawski, S.; Schmidt, K. [University of Silesia, Katowice (Poland); Kowalik, K.; Rondio, E.; Stepaniak, J. [National Centre for Nuclear Research, Warsaw (Poland); Laszlo, A.; Marton, K.; Vesztergombi, G. [Wigner Research Centre for Physics of the Hungarian Academy of Sciences, Budapest (Hungary); Lewicki, M.; Naskret, M.; Turko, L. [University of Wroclaw, Wroclaw (Poland); Messerly, B.; Paolone, V.; Wickremasinghe, A. [University of Pittsburgh, Pittsburgh (United States); Mills, G.B. [Los Alamos National Laboratory, Los Alamos (United States); Morozov, S.; Petukhov, O. [Institute for Nuclear Research, Moscow (Russian Federation); National Research Nuclear University (Moscow Engineering Physics Institute), Moscow (Russian Federation); Mrowczynski, S.; Rybczynski, M.; Seyboth, P.; Stefanek, G.; Wlodarczyk, Z.; Wojtaszek-Szwarc, A. [Jan Kochanowski University in Kielce, Kielce (Poland); Pavin, M. [Ruder Boskovic Institute, Zagreb (Croatia); University of Paris VI and VII, LPNHE, Paris (France); Popov, B.A. [University of Paris VI and VII, LPNHE, Paris (France); Joint Institute for Nuclear Research, Dubna (Russian Federation); Rauch, W. [Fachhochschule Frankfurt, Frankfurt (Germany); Roehrich, D. [University of Bergen, Bergen (Norway); Rustamov, A. [National Nuclear Research Center, Baku (Azerbaijan); University of Frankfurt, Frankfurt (Germany); Zambelli, L. [National Nuclear Research Center, Baku (Azerbaijan); University of Paris VI and VII, LPNHE, Paris (France); Collaboration: The NA61/SHINE Collaboration

2017-10-15

Measurements of inclusive spectra and mean multiplicities of π{sup ±}, K{sup ±}, p and p produced in inelastic p + p interactions at incident projectile momenta of 20, 31, 40, 80 and 158 GeV/c (√(s) = 6.3, 7.7, 8.8, 12.3 and 17.3 GeV, respectively) were performed at the CERN Super Proton Synchrotron using the large acceptance NA61/SHINE hadron spectrometer. Spectra are presented as function of rapidity and transverse momentum and are compared to predictions of current models. The measurements serve as the baseline in the NA61/SHINE study of the properties of the onset of deconfinement and search for the critical point of strongly interacting matter. (orig.)

Revealing the Dimeric Crystal and Solution Structure of β-Lactoglobulin at pH 4 and Its pH and Salt Dependent Monomer–Dimer Equilibrium

DEFF Research Database (Denmark)

Khan, Sanaullah; Ipsen, Richard; Almdal, Kristoffer

2018-01-01

The dimeric structure of bovine β-lactoglobulin A (BLGA) at pH 4.0 was solved to 2.0 Å resolution. Fitting the BLGA pH 4.0 structure to SAXS data at low ionic strength (goodness of fit R-factor = 3.6%) verified the dimeric state in solution. Analysis of the monomer–dimer equilibrium at varying pH...... and ionic strength by SAXS and scattering modeling showed that BLGA is dimeric at pH 3.0 and 4.0, shifting toward a monomer at pH 2.2, 2.6, and 7.0 yielding monomer/dimer ratios of 80/20%, 50/50%, and 25/75%, respectively. BLGA remained a dimer at pH 3.0 and 4.0 in 50–150 mM NaCl, whereas the electrostatic...... shielding raised the dimer content at pH 2.2, 2.6, and 7.0, i.e., below and above the pI. Overall, the findings provide new insights into the molecular characteristics of BLGA relevant for dairy product formulations and for various biotechnological and pharmaceutical applications....

Microbial Regulation of p53 Tumor Suppressor.

Directory of Open Access Journals (Sweden)

Alexander I Zaika

2015-09-01

Full Text Available p53 tumor suppressor has been identified as a protein interacting with the large T antigen produced by simian vacuolating virus 40 (SV40. Subsequent research on p53 inhibition by SV40 and other tumor viruses has not only helped to gain a better understanding of viral biology, but also shaped our knowledge of human tumorigenesis. Recent studies have found, however, that inhibition of p53 is not strictly in the realm of viruses. Some bacterial pathogens also actively inhibit p53 protein and induce its degradation, resulting in alteration of cellular stress responses. This phenomenon was initially characterized in gastric epithelial cells infected with Helicobacter pylori, a bacterial pathogen that commonly infects the human stomach and is strongly linked to gastric cancer. Besides H. pylori, a number of other bacterial species were recently discovered to inhibit p53. These findings provide novel insights into host-bacteria interactions and tumorigenesis associated with bacterial infections.

Study of giant multipole resonances in 40Ca

International Nuclear Information System (INIS)

Rost, H.

1979-01-01

In the present thesis giant resonance states in 40 Ca were studied by scattering of 104 MeV a particles on 40 Ca and by the reactions 39 K(p vector,p') 39 K and 39 K(p,α) 36 Ar. The scattered α-particles were measured at extreme forward angles (THETAsub(L) = 4 0 -16 0 C), because at forward angles the cross sections for the excitation of states with spin 0 and 1 strongly differ from those with higher spin. The aim of this experiment was first of all the study of the giant resonance region in 40 Ca on the contribution to 0 + or 1 - states. Beside the known electric giant quadrupole resonances at Esub(x) approx. equal to 18.5 MeV (25% EWSR) contributions of EO-strength at Esub(x) approx. equal to 21 MeV (6% EWSR) and indications to a (isoscalar) E1-strength at Esub(x) approx. equal to 14 MeV and Esub(x) approx. equal to 16 MeV were found. At the reactions 39 K(p vector,p') 39 K and 39 K(p,α) 36 Ar in the channels (p,p 0 ),(p,p 4 ), (p,αsub(o)), and (p,α 1 ) at incident energies at about 10 MeV (Esub(x)( 40 Ca) approx. equal to 18 MeV) resonant structures were observed. A scattering phase analysis performed for the elastic proton scattering didn't however yield quantitative results about the resonance parameter. An expansion of the cross sections by Legendre polynomials for the remaining reaction channel didn't allow a conclusion about the dominance of a certain L-value. The only indication to the connection of the observed resonant structures with the giant quadrupole resonance in 40 Ca is therefore the energetic position at about Esub(x) approx. equal to 18 MeV. Altogether the observed structures however were not very pronounced, so it can be concluded, that the excitation of the giant quadrupole resonance in 40 Ca by protons via the ground state of 39 K occurs not very strongly. (orig./HSI) [de

[Construction and identification of Nogo extra cellular peptide residues 1-40 gene lentiviral vector].

Science.gov (United States)

Yuan, Haifeng; Song, Yueming; Liu, Hao; Zhou, Chunguang; Kong, Qingquan; Liu, Liming; Gong, Quan

2012-02-01

To construct a lentiviral expression vector carrying Nogo extra cellular peptide residues 1-40 (NEP1-40) and to obtain NEP1-40 efficient and stable expression in mammalian cells. The DNA fragment of NEP1-40 coding sequence was amplified by PCR with designed primer from the cDNA library including NEP1-40 gene, and then subcloned into pGC-FU vector with in-fusion technique to generate the lentiviral expression vector, pGC-FU-NEP1-40. The positive clones were screened by PCR and the correct NEP1-40 was confirmed by sequencing. Recombinant lentiviruses were produced in 293T cells after the cotransfection of pGC-FU-NEP1-40, and packaging plasmids of pHelper 1.0 and pHelper 2.0. Green fluorescent protein (GFP) expression of infected 293T cells was observed to evaluate gene delivery efficiency. NEP1-40 protein expression in 293T cells was detected by Western blot. The lentiviral expression vector carrying NEP1-40 was successfully constructed by GFP observation, and NEP1-40 protein expression was detected in 293T cells by Western blot. The recombinant lentivirus pGC-FU-NEP1-40 is successfully constructed and it lays a foundation for further molecular function study of NEP 1-40.

Unitary Pole Approximation For 16O S12state And 40ca P32state When Coulomb Interaction Is Included

Directory of Open Access Journals (Sweden)

A. Acharya

2015-08-01

Full Text Available Abstract The form factor of a separable interaction between a pair of particles is an important input in a three body calculation for a transfer reaction. The three body equations of Alt Grassberger and Sandhas have been solved for a system of three particles viz.p n and 16Oand p n and 40Ca when coulomb interaction is included between the particle pairs. The input in this calculation i.e. the two body t-matrices representing the interaction between the pairs of particles is taken to be of a separable form conforming to the bound state of the pair. The form factors of the total interaction between the particle pairs are constructed using the prescription of Ueta and Bund.

Dimerization of A-[alpha]-[SiNb3W9O40]7- by pH-controlled formation of individual Nb−µ-O−Nb linkages

Science.gov (United States)

Gyu-Shik Kim; Huadong Zeng; Wade A. Neiwert; Jennifer J. Cowan; Donald VanDerveer; Craig L. Hill; Ira A. Weinstock

2003-01-01

The reversible, stepwise formation of individual Nb−µ-O−Nb linkages during acid condensation of 2 equiv of A-[alpha]-[SiNb3W9O40]7- (1) to the tri-µ-oxo-bridged structure A-[alpha]-[Si2Nb6W18O77]8- (4) is demonstrated by a combination of X-ray crystallography and variable-pD solution 183W and 29Si NMR spectroscopy. Addition of DCl to a pD 8.4...

Purinergní P2X rodina a specifické vlastnosti P2X7 podtypu

Czech Academy of Sciences Publication Activity Database

Jindřichová, Marie; Zemková, Hana

2013-01-01

Roč. 62, č. 2 (2013), s. 40-46 ISSN 1210-6313 R&D Projects: GA ČR(CZ) GPP304/12/P371 Institutional support: RVO:67985823 Keywords : extracellular ATP * purinergic P2X family * P2X7 receptor * cell proliferation and apoptosis Subject RIV: ED - Physiology

Serum YKL-40 in risk assessment for colorectal cancer

DEFF Research Database (Denmark)

Johansen, Julia Sidenius; Christensen, Ib Jarle; Jørgensen, Lars Nannestad

2015-01-01

The aim of the present study was to test the hypothesis that high serum YKL-40 associates with colorectal cancer in subjects at risk of colorectal cancer. We measured serum YKL-40 in a prospective study of 4,496 Danish subjects [2,064 men, 2,432 women, median age 61 years (range, 18-97)] referred.......05-1.40; P = 0.012), whereas this was not the case for those with comorbidity (OR, 0.98; 95% CI, 0.84-1.14; P = 0.80). In conclusion, high serum YKL-40 in subjects suspected of colorectal cancer and without comorbidity associates with colorectal cancer. Determination of serum YKL-40 may be useful...

The clinical investigation of OX40/OX40L from lymphocytes of patients with Graves' disease after 131I therapy

International Nuclear Information System (INIS)

Shi Bimin; Du Xuan; Wang Qin

2013-01-01

Objective To investigate the clinical value and the expression of OX40/OX40L from lymphocytes of patients with Graves' disease (GD) before and after 131 I therapy.Methods The expression of OX40/OX40L on T,B lymphocytes and the percentage of lymphocyte subsets were analyzed using immunofluorescence staining and flow cytometry in 32 patients with GD before and after 131 I therapy.Twenty-five healthy subjects were considered as the control group.The data between GD patients and controls were compared with two-sample t test.The correlation between the expression of OX40/OX40L and the function of thyroid (FT3,FT4 and TRAb level) was performed with linear correlation analysis.Results Compared with the healthy subjects,the percentage of CD4+ T cells and ratio of CD4+/CD8+ were decreased ((34.36 ±6.73)% vs (45.60-±5.72)%,t=2.634; 1.24±0.26 vs 1.84±0.37,t=2.125,both P<0.05).Whereas the percentage of CD19+ B cells in patients with GD were significantly higher than that in healthy subjects ((21.42 ±3.92)% vs (15.35 ± 3.15)%,t =2.653,P<0.05).The expression of OX40 increased in CD4+ T lymphocytes ((12.92 ± 2.26) %) and OX40L expression was up-regulated in CD19+ B lymphocytes ((15.33 ± 3.75)%) of patients with GD,compared with the healthy subjects ((4.19 ±1.45) % and (8.64 ± 1.73) %,respectively,t =2.112 and 2.467,both P<0.05).The expression of OX40 in CD4+ T lymphocytes ((7.65 ± 1.64) %) and OX40L in CD19+ B lymphocytes ((11.50 ±2.72)%) were increased after 131 I therapy(t =2.795,2.393,both P<0.05).The thyroid functions of 32 GD patients were improved.The levels of FT3,FT4 and TRAb decreased significantly after 131 I treatment (from 20.79 ±6.05 to 4.53 ± 2.54,from 49.65 ± 10.12 to 13.69 ± 4.35,and from 24.78 ± 8.46 to 11.74 ±6.19,respectively; t =2.219,2.441 and 2.293,all P<0.05).The levels of FT3 and FT4 were positively correlated with the percentage of OX40 expression in CD4+ T lymphocytes (r =0.65 and 0.73,both P<0.05).TRAb was positively correlated

Optimum pH and pH Stability of Crude Polyphenol Oxidase (PPO ...

African Journals Online (AJOL)

The effect of pH on the activity and stability of crude polyphenol oxidase (PPO) extracted from garden egg (Solanum aethiopicum), pawpaw (Carica papaya), pumpkin ... Optimum pH values were found to be 6.0,6.5,6.0, 4.5 and 4.0/or 8.0 for the enzyme extracted from Solanum aethiopicum, Carica papaya, Cucurbita pepo, ...

Involvement of nuclear factor κB in platelet CD40 signaling

International Nuclear Information System (INIS)

Hachem, Ahmed; Yacoub, Daniel; Zaid, Younes; Mourad, Walid; Merhi, Yahye

2012-01-01

Highlights: ► sCD40L induces TRAF2 association to CD40 and NF-κB activation in platelets. ► IκBα phosphorylation downstream of CD40L/CD40 signaling is independent of p38 MAPK phosphorylation. ► IκBα is required for sCD40L-induced platelet activation and potentiation of aggregation. -- Abstract: CD40 ligand (CD40L) is a thrombo-inflammatory molecule that predicts cardiovascular events. Platelets constitute the major source of soluble CD40L (sCD40L), which has been shown to potentiate platelet activation and aggregation, in a CD40-dependent manner, via p38 mitogen activated protein kinase (MAPK) and Rac1 signaling. In many cells, the CD40L/CD40 dyad also induces activation of nuclear factor kappa B (NF-κB). Given that platelets contain NF-κB, we hypothesized that it may be involved in platelet CD40 signaling and function. In human platelets, sCD40L induces association of CD40 with its adaptor protein the tumor necrosis factor receptor associated factor 2 and triggers phosphorylation of IκBα, which are abolished by CD40L blockade. Inhibition of IκBα phosphorylation reverses sCD40L-induced IκBα phosphorylation without affecting p38 MAPK phosphorylation. On the other hand, inhibition of p38 MAPK phosphorylation has no effect on IκBα phosphorylation, indicating a divergence in the signaling pathway originating from CD40 upon its ligation. In functional studies, inhibition of IκBα phosphorylation reverses sCD40L-induced platelet activation and potentiation of platelet aggregation in response to a sub-threshold concentration of collagen. This study demonstrates that the sCD40L/CD40 axis triggers NF-κB activation in platelets. This signaling pathway plays a critical role in platelet activation and aggregation upon sCD40L stimulation and may represent an important target against thrombo-inflammatory disorders.

Characterization of a recurrent t(1;2)(p36;p24) in human uterine leiomyoma.

NARCIS (Netherlands)

Rijk, A. van; Sweers, M.A.; Huys, E.; Kersten, M.; Merkx, G.F.M.; Geurts van Kessel, A.H.M.; Debiec-Rychter, M.; Schoenmakers, E.F.P.M.

2009-01-01

Uterine leiomyomas are the most common neoplasms in women of reproductive age. Approximately 40% of these neoplasms show recurring structural cytogenetic anomalies, including del(7)(q22), t(12;14)(q15;q24), t(1;2)(p36;p24), and anomalies affecting 6p21 or 10q22. Using positional cloning strategies,

Measurements of $$\\pi ^\\pm $$ π ± , K $$^\\pm $$ ± , p and $${\\bar{\\text {p}}}$$ p ¯ spectra in proton-proton interactions at 20, 31, 40, 80 and 158  $$\\text{ GeV }/c$$ GeV / c with the NA61/SHINE spectrometer at the CERN SPS: The NA61/SHINE Collaboration

Energy Technology Data Exchange (ETDEWEB)

Aduszkiewicz, A.; Ali, Y.; Andronov, E.; Antićić, T.; Baatar, B.; Baszczyk, M.; Bhosale, S.; Blondel, A.; Bogomilov, M.; Brandin, A.; Bravar, A.; Brzychczyk, J.; Bunyatov, S. A.; Busygina, O.; Cherif, H.; Ćirković, M.; Czopowicz, T.; Damyanova, A.; Davis, N.; Dembinski, H.; Deveaux, M.; Dominik, W.; Dorosz, P.; Dumarchez, J.; Engel, R.; Ereditato, A.; Feofilov, G. A.; Fodor, Z.; Francois, C.; Garibov, A.; Gaździcki, M.; Golubeva, M.; Grebieszkow, K.; Guber, F.; Haesler, A.; Hervé, A. E.; Hylen, J.; Igolkin, S.; Ivashkin, A.; Johnson, S. R.; Kadija, K.; Kaptur, E.; Kiełbowicz, M.; Kireyeu, V. A.; Klochkov, V.; Knezević, N.; Kolesnikov, V. I.; Kolev, D.; Kondratiev, V. P.; Korzenev, A.; Kovalenko, V.; Kowalik, K.; Kowalski, S.; Koziel, M.; Krasnoperov, A.; Kucewicz, W.; Kuich, M.; Kurepin, A.; Larsen, D.; László, A.; Lewicki, M.; Lundberg, B.; Lyubushkin, V. V.; Łysakowski, B.; Maćkowiak-Pawłowska, M.; Maksiak, B.; Malakhov, A. I.; Manić, D.; Marchionni, A.; Marcinek, A.; Marino, A. D.; Marton, K.; Mathes, H. -J.; Matulewicz, T.; Matveev, V.; Melkumov, G. L.; Merzlaya, A.; Messerly, B.; Mik, Ł.; Mills, G. B.; Morozov, S.; Mrówczyński, S.; Nagai, Y.; Naskręt, M.; Ozvenchuk, V.; Paolone, V.; Pavin, M.; Petukhov, O.; Pistillo, C.; Płaneta, R.; Podlaski, P.; Popov, B. A.; Posiadała, M.; Puławski, S.; Puzović, J.; Rameika, R.; Rauch, W.; Ravonel, M.; Renfordt, R.; Richter-Wąs, E.; Röhrich, D.; Rondio, E.; Roth, M.; Rumberger, B. T.; Rustamov, A.; Rybczynski, M.; Rybicki, A.; Sadovsky, A.; Schmidt, K.; Selyuzhenkov, I.; Seryakov, A.; Seyboth, P.; Słodkowski, M.; Snoch, A.; Staszel, P.; Stefanek, G.; Stepaniak, J.; Strikhanov, M.; Ströbele, H.; Šuša, T.; Szuba, M.; Taranenko, A.; Tefelska, A.; Tefelski, D.; Tereshchenko, V.; Toia, A.; Tsenov, R.; Turko, L.; Ulrich, R.; Unger, M.; Veberič, D.; Vechernin, V. V.; Vesztergombi, G.; Vinogradov, L.; Walewski, M.; Wickremasinghe, A.; Wilkinson, C.; Włodarczyk, Z.; Wojtaszek-Szwarc, A.; Wyszyński, O.; Zambelli, L.; Zimmerman, E. D.; Zwaska, R.

2017-10-01

Measurements of inclusive spectra and mean multiplicities of $\\pi^\\pm$, K$^\\pm$, p and $\\bar{\\textrm{p}}$ produced in inelastic p+p interactions at incident projectile momenta of 20, 31, 40, 80 and 158 GeV/c ($\\sqrt{s} = $ 6.3, 7.7, 8.8, 12.3 and 17.3 GeV, respectively) were performed at the CERN Super Proton Synchrotron using the large acceptance NA61/SHINE hadron spectrometer. Spectra are presented as function of rapidity and transverse momentum and are compared to predictions of current models. The measurements serve as the baseline in the NA61/SHINE study of the properties of the onset of deconfinement and search for the critical point of strongly interacting matter.

Differential protein expression, DNA binding and interaction with SV40 large tumour antigen implicate the p63-family of proteins in replicative senescence.

Science.gov (United States)

Djelloul, Siham; Tarunina, Marina; Barnouin, Karin; Mackay, Alan; Jat, Parmjit S

2002-02-07

P53 activity plays a key role in mammalian cells when they undergo replicative senescence at their Hayflick limit. To determine whether p63 proteins, members of the family of p53-related genes, are also involved in this process, we examined their expression in serially passaged rat embryo fibroblasts. Upon senescence, two truncated DeltaNp63 proteins decreased in abundance whereas two TAp63 isoforms accumulated. 2-D gel analysis showed that the DeltaNp63 proteins underwent post-translational modifications in both proliferating and senescent cells. Direct binding of DeltaNp63 proteins to a p53 consensus motif was greater in proliferating cells than senescent cells. In contrast p63alpha isoforms bound to DNA in a p53 dependent manner and this was higher in senescent cells than proliferating cells. An interaction of p63alpha proteins with SV40 large tumour antigen was also detected and ectopic expression of DeltaNp63alpha can extend the lifespan of rat embryo fibroblasts. Taken together the results indicate that p63 proteins may play a role in replicative senescence either by competition for p53 DNA binding sites or by direct interaction with p53 protein bound to DNA.

Effective nucleon-nucleon t matrix in the (p,2p) reaction

International Nuclear Information System (INIS)

Kudo, Y.; Kanayama, N.; Wakasugi, T.

1989-01-01

The cross sections and the analyzing powers for the /sup 40/Ca(p-arrow-right,2p) reactions at E/sub p/ = 76.1, 101.3, and 200 MeV are calculated in the distorted-wave impulse approximation using the Love-Franey effective nucleon-nucleon interaction. It is shown that the calculated individual contributions of the central, spin-orbit, and tensor parts in the Love-Franey interaction to the cross sections and the analyzing powers strongly depend on the incident proton energies. The spectroscopic factors extracted are consistent with the other reaction studies

Involvement of nuclear factor {kappa}B in platelet CD40 signaling

Energy Technology Data Exchange (ETDEWEB)

Hachem, Ahmed [Laboratory of Thrombosis and Hemostasis, Montreal Heart Institute, 5000 Belanger, Montreal, Quebec, Canada H1T 1C8 (Canada); Yacoub, Daniel [Laboratory of Thrombosis and Hemostasis, Montreal Heart Institute, 5000 Belanger, Montreal, Quebec, Canada H1T 1C8 (Canada); Centre Hospitalier Universite de Montreal, 264 boul. Rene-Levesque est, Montreal, Quebec, Canada H2X 1P1 (Canada); Zaid, Younes [Laboratory of Thrombosis and Hemostasis, Montreal Heart Institute, 5000 Belanger, Montreal, Quebec, Canada H1T 1C8 (Canada); Mourad, Walid [Universite de Montreal, Department of Medicine, 2900 boul. Edouard-Montpetit, Montreal, Quebec, Canada H3T 1J4 (Canada); Centre Hospitalier Universite de Montreal, 264 boul. Rene-Levesque est, Montreal, Quebec, Canada H2X 1P1 (Canada); Merhi, Yahye, E-mail: yahye.merhi@icm-mhi.org [Laboratory of Thrombosis and Hemostasis, Montreal Heart Institute, 5000 Belanger, Montreal, Quebec, Canada H1T 1C8 (Canada); Universite de Montreal, Department of Medicine, 2900 boul. Edouard-Montpetit, Montreal, Quebec, Canada H3T 1J4 (Canada)

2012-08-17

Highlights: Black-Right-Pointing-Pointer sCD40L induces TRAF2 association to CD40 and NF-{kappa}B activation in platelets. Black-Right-Pointing-Pointer I{kappa}B{alpha} phosphorylation downstream of CD40L/CD40 signaling is independent of p38 MAPK phosphorylation. Black-Right-Pointing-Pointer I{kappa}B{alpha} is required for sCD40L-induced platelet activation and potentiation of aggregation. -- Abstract: CD40 ligand (CD40L) is a thrombo-inflammatory molecule that predicts cardiovascular events. Platelets constitute the major source of soluble CD40L (sCD40L), which has been shown to potentiate platelet activation and aggregation, in a CD40-dependent manner, via p38 mitogen activated protein kinase (MAPK) and Rac1 signaling. In many cells, the CD40L/CD40 dyad also induces activation of nuclear factor kappa B (NF-{kappa}B). Given that platelets contain NF-{kappa}B, we hypothesized that it may be involved in platelet CD40 signaling and function. In human platelets, sCD40L induces association of CD40 with its adaptor protein the tumor necrosis factor receptor associated factor 2 and triggers phosphorylation of I{kappa}B{alpha}, which are abolished by CD40L blockade. Inhibition of I{kappa}B{alpha} phosphorylation reverses sCD40L-induced I{kappa}B{alpha} phosphorylation without affecting p38 MAPK phosphorylation. On the other hand, inhibition of p38 MAPK phosphorylation has no effect on I{kappa}B{alpha} phosphorylation, indicating a divergence in the signaling pathway originating from CD40 upon its ligation. In functional studies, inhibition of I{kappa}B{alpha} phosphorylation reverses sCD40L-induced platelet activation and potentiation of platelet aggregation in response to a sub-threshold concentration of collagen. This study demonstrates that the sCD40L/CD40 axis triggers NF-{kappa}B activation in platelets. This signaling pathway plays a critical role in platelet activation and aggregation upon sCD40L stimulation and may represent an important target against thrombo

Comparative genomics of the pIPO2/pSB102 family of environmental plasmids : sequence, evolution, and ecology of pTer331 isolated from Collimonas fungivorans Ter331

NARCIS (Netherlands)

Mela, Francesca; Fritsche, Kathrin; Boersma, Hidde; van Elsas, Jan D.; Bartels, Daniela; Meyer, Folker; de Boer, Wietse; van Veen, Johannes A.; Leveau, Johan H. J.

2008-01-01

Plasmid pTer331 from the bacterium Collimonas fungivorans Ter331 is a new member of the pIPO2/pSB102 family of environmental plasmids. The 40 457-bp sequence of pTer331 codes for 44 putative ORFs, most of which represent genes involved in replication, partitioning and transfer of the plasmid. We

Separation of the transverse and longitudinal structure functions for the (e,e'p) coincidence reaction on the 40Ca nucleus

International Nuclear Information System (INIS)

Reffay-Pikeroen, Dominique

1987-01-01

The bound-nucleon current has been investigated in the region of the quasi-elastic peak, with (e,e'p) coincidence measurements where by the one-nucleon knock-out process is selected. This study is refined by the measurement of the separate transverse and longitudinal structure functions. The (e,e'p) reaction has been performed on the 40 Ca nucleus in a momentum transfer range from 330 to 825 MeV/c. We have chosen a range of proton momenta in the nucleus from 40 to 140 MeV and a missing energy range from 8 to 60 MeV which corresponds to the shells 1d3/2, 1d5/2, 2s1/2, 1s1/2, 1p3/2, 1p1/2 of 39 K as predicted by the shell model. The first aim of these measurements was to verify, on an exclusive process, the results of the inclusive measurements on this nucleus with transverse/longitudinal separation, since the inclusive results are presently difficult to interpret. More generally, the aim of this kind of measurements is to test the validity of the traditional theoretical approach to the quasi-elastic scattering: Schroedinger equation, impulse approximation, choice of a prescription for the off shell effect on the nucleonic current keeping the free nucleon electromagnetic form factors. The answer to the first question is the observation of a quenching of the longitudinal/transverse ratio, consistent with the effect observed in the inclusive experiments. Regarding the most general aspect of the study of the electromagnetic structure of the bound nucleus, this experiment has brought some entirely new results about the momentum transfer dependence of its electric and magnetic form factors. These results do not suggest any important deformation of the nucleon in the nucleus. We were able to derive an upper limit of 4 pc for a possible increase of the magnetic radius of the bound proton. Regarding the electric radius, the data still remain too inaccurate to draw final conclusions. (author) [fr

p53-Induced Apoptosis Occurs in the Absence of p14ARF in Malignant Pleural Mesothelioma

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Sally Hopkins-Donaldson

2006-07-01

Full Text Available Malignant pleural mesotheliomas (MPMs are usually wild type for the p53 gene but contain homozygous deletions in the INK4A locus that encodes p14ARF, an inhibitor of p53-MDM2 interaction. Previous findings suggest that lack of p14ARF expression and the presence of SV40 large T antigen (L-Tag result in p53 inactivation in MPM. We did not detect SV40 L-Tag mRNA in either MPM cell lines or primary cultures, treatment of p14ARF-deficient cells with cisplatin (CDDP increased both total and phosphorylated p53 and enhanced p53 DNA-binding activity. On incubation with CDDP, levels of positively regulated p53 transcriptional targets p21WAF, PIG3, MDM2, Bax, PUMA increased in p14ARF-deficient cells, whereas negatively regulated survivin decreased. Significantly, p53-induced apoptosis was activated by CDDP in p14ARF-deficient cells, treatment with p53-specific siRNA rendered them more CDDP-resistant. p53 was also activated by: 1 inhibition of MDM2 (using nutlin-3; 2 transient overexpression of p14ARF; and 3 targeting of survivin using antisense oligonucleotides. However, it is noteworthy that only survivin downregulation sensitized cells to CDDP-induced apoptosis. These results suggest that p53 is functional in the absence of p14ARF in MPM and that targeting of the downstream apoptosis inhibitor survivin can sensitize to CDDP-induced apoptosis.

Elastic scattering analysis of p + 40Ca at incident energies from threshold to 48.4 MeV and nuclear matter radius

International Nuclear Information System (INIS)

Ismail, Atef; Lee, Yen Cheong; Tammam, M.

2015-01-01

Proton elastic scattering at various incident energies is one method to study nuclear density distributions and nuclear radii. Single folding potential describing the p-scattering on 40 Ca over a broad energy range 9–48.4 MeV is constructed. The resulting potential does not need any renormalization to fit the measured elastic scattering angular distributions and total reaction cross-sections. Furthermore, correlation between volume integral and proton incident energy is discussed. Theoretical calculations are in a good agreement with existing experimental data. (author)

SERUM YKL-40 IS ASSOCIATED WITH BONE DISEASE IN MULTIPLE MYELOMA

DEFF Research Database (Denmark)

Mylin, Anne Kjærsgaard; Abildgaard, Niels; Johansen, Julia S.

2007-01-01

 Introduction. The secreted glycoprotein YKL-40 (CHI3L1, HC gp-39) is a potential player in the tumor-host interactions affecting several aspects of multiple myeloma (MM) including bone destruction. Previous studies support a role for YKL-40 in remodelling of the extracellular matrix, in angiogen...... Introduction. The secreted glycoprotein YKL-40 (CHI3L1, HC gp-39) is a potential player in the tumor-host interactions affecting several aspects of multiple myeloma (MM) including bone destruction. Previous studies support a role for YKL-40 in remodelling of the extracellular matrix...... and followed for up to 30 months. Skeletal related events (SRE) were registered and subdivided in vertebral fractures and osteolytic events including non-vertebral fractures. Results. 57% of the patients had a S-YKL-40 elevated above the upper limit in an age specific 90 per cent reference range for healthy...... adults. Patients with elevated S-YKL-40 had a higher total X-ray score (p=0.005) and higher levels of S-CTX-MMP (p=0.003), U-PYD (p=0.004) and U-DPD (p=0.002), while U-NTX-1 and the markers of bone formation did not differ from the levels seen in patients with normal S-YKL-40. During follow-up 21...

Anodic photodissolution of n-InP, under electroless conditions

International Nuclear Information System (INIS)

Debiemme-Chouvy, Catherine; Quennoy, Anne

2004-01-01

In the presence of α-SiMo 12 O 40 4- ions dissolved in acidic solution and under laser irradiation, the electroless photoetching of n-type InP is achieved. At the laser impact, the semiconductor is oxidized while SiMo 12 O 40 4- species are reduced. The shape of the pit formed, due to the photoanodic dissolution of the material, depends on the experimental conditions, notably on the presence or not of Cl - ions in the medium. It can have either a Gaussian shape or a flat bottom. To specify the charge transfer which occurs at the n-InP/solution illuminated interface, some electrochemical studies were performed on n- and p-type InP electrodes. In fact, the reduction of SiMo 12 O 40 4- ions occurs by capture of electrons from the InP conduction band. Considering the energetic situation at the InP/electrolyte interface and some electrochemical results, it is concluded that the electron transfer from InP to SiMo 12 is mediated by surface states. The influence of Cl - ions on the n-InP photodissolution process is also discussed

Structural Analysis and Aggregation Propensity of Pyroglutamate Aβ(3-40 in Aqueous Trifluoroethanol.

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Christina Dammers

Full Text Available A hallmark of Alzheimer's disease (AD is the accumulation of extracellular amyloid-β (Aβ plaques in the brains of patients. N-terminally truncated pyroglutamate-modified Aβ (pEAβ has been described as a major compound of Aβ species in senile plaques. pEAβ is more resistant to degradation, shows higher toxicity and has increased aggregation propensity and β-sheet stabilization compared to non-modified Aβ. Here we characterized recombinant pEAβ(3-40 in aqueous trifluoroethanol (TFE solution regarding its aggregation propensity and structural changes in comparison to its non-pyroglutamate-modified variant Aβ(1-40. Secondary structure analysis by circular dichroism spectroscopy suggests that pEAβ(3-40 shows an increased tendency to form β-sheet-rich structures in 20% TFE containing solutions where Aβ(1-40 forms α-helices. Aggregation kinetics of pEAβ(3-40 in the presence of 20% TFE monitored by thioflavin-T (ThT assay showed a typical sigmoidal aggregation in contrast to Aβ(1-40, which lacks ThT positive structures under the same conditions. Transmission electron microscopy confirms that pEAβ(3-40 aggregated to large fibrils and high molecular weight aggregates in spite of the presence of the helix stabilizing co-solvent TFE. High resolution NMR spectroscopy of recombinantly produced and uniformly isotope labeled [U-15N]-pEAβ(3-40 in TFE containing solutions indicates that the pyroglutamate formation affects significantly the N-terminal region, which in turn leads to decreased monomer stability and increased aggregation propensity.

Reduction of mercury (II) by humic substances-influence of pH, salinity of aquatic system

Digital Repository Service at National Institute of Oceanography (India)

Chakraborty, P.; Vudamala, K.; Coulibaly, M.; Ramteke, D.; Chennuri, K.; Lean, D.

60 80 pH 4.0 pH 7.0 pH 8.0 pH 9.0 Time (h-1) 0 20 40 60 80 100 120 140 160 0 10 20 30 40 pH 4.0 pH 5.0 pH 6.0 pH 7.0 pH 8.0 pH 9.0 Figure 5 a b c Pe rc en ta ge o f t he to ta l d is so lv ed H g re du ce d b y H S an d tr ap ed in o xi di... humic substances (wt %) 0.42 0.44 0.46 0.48 0.50 0.52 0.54 0.56 Fr ac tio n (p er ce nt ag e) o f t ot al H g re du ce d by H S 20 30 40 50 60 70 Salinity = 5 PSU Salinity = 10 PSU Salinity = 15 PSU Salinity = 20PSU Salinity = 30 PSU 0.42 0...

Reaction π-p → π0n in the 15-40 GeV/c momentum range

International Nuclear Information System (INIS)

Apel, W.D.; Augenstein, K.H.; Krueger, M.; Mueller, H.; Schinzel, D.; Schneider, H.; Sigurdsson, G.; Bertolucci, E.; Mannelli, I.; Pierazzini, G.M.; Quaglia, M.; Scribano, A.; Sergiampietri, F.; Vincelli, M.L.; Donskov, S.V.; Inyakin, A.V.; Johnson, R.; Kachanov, V.A.; Krasnokutsky, R.N.; Lednev, A.A.; Mikhailov, Yu.V.; Prokoshkin, Yu. D.; Shuvalov, R.S.; Toropin, A.N.; Leder, G.; Steuer, M.

1979-01-01

A high statistics measurement of the reaction π - p → π 0 n has been performed at the Serpukhov accelerator for 15, 20, 25, 30 and 40 GeV/c incident pion momentum using the NICE set-up with its associated 648-channel hodoscope spectrometer for γ-ray detection. More than 3 million charge-exchange events have been recorded in total. It is found that the spin-flip and non-spin-flip amplitudes can be parametrized, for small mod(t) as exponentials with the same slopes to within a few per cent. Also the behaviour of the differential cross section for small and medium mod(t) agrees with the prediction of a geometrical s-channel model which describes binary reactions in terms of a complex pole b 0 (s). The imaginary part of this universal pole, Im b 0 (s), has been determined and found to be growing logarithmically with s. (Auth.)

Spin dipole and quadrupole resonances in 40Ca

International Nuclear Information System (INIS)

Baker, F.T.; Love, W.G.; Bimbot, L.; Fergerson, R.W.; Glashausser, C.; Green, A.; Jones, K.; Nanda, S.

1989-01-01

Angular distributions of the double differential cross section d 2 σ/dΩ dE(σ) and the spin-flip probability S nn have been measured for inclusive proton inelastic scattering from 40 Ca at 319 MeV. Excitation energies (ω) up to about 40 MeV have been investigated over the angular range from 3.5 degree to 12 degree in the laboratory (0.3 to 0.9 fm -1 ). Here, multipole decompositions of angular distributions of σS nn for the 40 Ca(rvec p,rvec p ') reaction at 319 MeV have been performed in order to compare ΔS=1 strength observed with sum rules. In contrast to the well-known quenching of Gamow-Teller and M1 resonances, the spin-dipole resonance has a total measured strength which is larger than that predicted by the energy-weighted sum rule. The spin-dipole strength distribution supports asymmetric widths predicted by calculations including 2p-2h mixing. The spin-quadrupole resonance is observed near ω=35 MeV and its total strength for ω<40 MeV estimated

Labour 4.0: Kompetencer til fremtidens industri

DEFF Research Database (Denmark)

Wæhrens, Brian Vejrum; Lassen, Astrid Heidemann; Nørgaard, Bente

2018-01-01

I Danmark fremhæves Region Nordjylland ofte som et område, der har et stort, men også et uudnyttet potentiale i forhold til mulighederne i det nye industriparadigme Industri 4.0. I regionen er der kompetencer og viden fra både den traditionelle fremstillingsindustri og fra IT-klyngen, der...... blomstrede omkring mobilindustrien i 1990´erne og siden har dannet grobund for mange nye virksomheder indenfor trådløs-/kommunikationsteknologi i mange andre industrier. Industri 4.0 bygger netop på at integrere digitalisering, automatisering og robotteknologi med traditionelle fremstillingsindustrier, og på...... den måde globalt skabe konkurrencedygtige og fleksible virksomheder, der i mange tilfælde med fordel igen vil kunne hjemtage produktionsopgaver fra lavtlønslande til Danmark og Nordjylland. Evnen til at omsætte Industri 4.0 potentialerne har på den måde vidtrækkende konsekvenser for Danmarks fremtid...

Thermal stability of amorphous structure and magnetic properties Fe{sub 80-x}Co{sub x}P{sub 14}B {sub 6} (x=20-40) ribbons

Energy Technology Data Exchange (ETDEWEB)

Kostyrya, S.A.; Idzikowski, B. [Institute of Molecular Physics, Polish Academy of Sciences, M. Smoluchowskiego 17, 60-179 Poznan (Poland); Tkatch, V.I.; Popov, V.V.; Rassolov, S.G. [Donetsk Physics and Engineering Institute of National Academy of Sciences of Ukraine, R. Luxemburg 72, 83114 Donetsk (Ukraine)

2006-01-01

Amorphous structure and its thermal stability as well as magnetic properties of Fe{sub 80-x}Co{sub x}P{sub 14}B{sub 6} (20{<=}x{<=}40) glasses have been studied by X-ray diffraction, non-isothermal differential scanning calorimetry (DSC) and thermomagnetic (TMG) measurements. The Curie temperature increases with Co content, while the glass transition and crystallization onset temperatures decrease. As a result, for alloys x=30, 32 and 35 at%, the transition into paramagnetic state take place close to glass transition temperature. However, for x=40 at% the TMG curve, in the vicinity of magnetic transition temperature, is overlapped by ferromagnetic crystallization products. (copyright 2006 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

Serum YKL-40 independently predicts outcome after transcatheter arterial chemoembolization of hepatocellular carcinoma.

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Cheng-Bao Zhu

Full Text Available Transcatheter arterial chemoembolization (TACE is the most widely used treatment option for unresectable hepatocellular carcinoma (HCC. Elevated serum YKL-40 level has been shown to predict poor prognosis in HCC patients undergoing resection. This study was designed to validate the prognostic significance of serum YKL-40 in patients with HCC undergoing TACE treatment.Serum YKL-40 level was determined by enzyme-linked immunosorbent assay. Overall survival (OS was evaluated with the Kaplan-Meier method and compared by the log-rank test. Multivariate study with Cox proportional hazard model was used to evaluate independent prognostic variables of OS.The median pretreatment serum YKL-40 in HCC patients with was significantly higher than that in healthy controls (P<0.001. The YKL-40 could predict survival precisely either in a dichotomized or continuous fashion (P<0.001 and P = 0.001, respectively. Multivariate Cox regression analysis indicated that serum YKL-40 was an independent prognostic factor for OS in HCC patients (P = 0.001. In further stratified analyses, YKL-40 could discriminate the outcomes of patients with low and high alpha-fetoprotein (AFP level (P = 0.006 and 0.016, respectively. Furthermore, the combination of serum YKL-40 and AFP had more capacity to predict patients' outcomes.Serum YKL-40 was demonstrated to be an independent prognostic biomarker in HCC patients treated with TACE. Our results need confirmation in an independent study.

Plasma YKL-40 in Inuit and Danes.

Science.gov (United States)

Nøjgaard, Camilla; Johansen, Julia S; Bjerregaard, Peter; Bojesen, Stig E; Becker, Ulrik

2015-01-01

The aim of the present study was to investigate whether there are differences in plasma levels of YKL-40 between Inuit in Greenland and in Denmark and in Danes, as well as to study the relationship between alcohol intake, plasma YKL-40 and other factors in Inuit. Plasma YKL-40 levels were measured on 1645 people from The Greenland Population Study (a cross-sectional population study of Inuit from Denmark and West Greenland) and were compared with the plasma YKL-40 levels of 8899 people from The Copenhagen City Heart Study (a population-based, prospective study of the Danish general population). The plasma concentrations of YKL-40 were significantly (P = 0.001) lower in Inuit living in Greenland (median 46 µg/l, range 10-2164, n = 1164) compared with the plasma YKL-40 levels of Inuit living in Denmark (median 63 µg/l, range 20-2827, n = 481) and of Danes living in Denmark (median 55 µg/l, range 10-2909, n = 8899). In Inuit, increased alcohol intake was significantly associated with increased plasma YKL-40 levels (P Inuit living in Greenland than Inuit and Danes living in Denmark. A number of factors, including different alcohol intake patterns, nutrition and genes may play a role in these findings. © The Author 2014. Medical Council on Alcohol and Oxford University Press. All rights reserved.

Impact of the Ki-67 labeling index and p53 expression status on disease-free survival in pT1 urothelial carcinoma of the bladder.

Science.gov (United States)

Vetterlein, Malte W; Roschinski, Julia; Gild, Philipp; Marks, Phillip; Soave, Armin; Doh, Ousman; Isbarn, Hendrik; Höppner, Wolfgang; Wagner, Walter; Shariat, Shahrokh F; Brausi, Maurizio; Büscheck, Franziska; Sauter, Guido; Fisch, Margit; Rink, Michael

2017-12-01

The identification of protein biomarkers to guide treatment decisions regarding adjuvant therapies for high-risk non-muscle-invasive bladder cancer (NMIBC) has been of increasing interest. Evidence of the impact of tumor suppressor gene product p53 and cell proliferation marker Ki-67 on oncologic outcomes in bladder cancer patients at highest risk of recurrence and progression is partially contradictory. We sought to mirror contemporary expression patterns of p53 and Ki-67 in a select cohort of patients with pT1 bladder cancer. Patients from four Northern German institutions with a primary diagnosis of pT1 bladder cancer between 2009 and 2016 and complete data regarding p53 or Ki-67 expression status were included for final analyses. Baseline patient characteristics (age, gender, age-adjusted Charlson comorbidity index) and tumor characteristics [diagnostic sequence, tumor focality, concomitant carcinoma in situ, 1973 World Health Organization (WHO) grading, lymphovascular invasion, adjuvant instillation therapy] were abstracted by retrospective chart review. Immunohistochemistry for detection of p53 and Ki-67 expression was performed according to standardized protocols. Microscopic analyses were performed by central pathologic review. First, we compared patients with positive vs. negative p53 expression and Ki-67 labeling index [>40% vs. ≤40%; cutoffs based on best discriminative ability in univariable Cox regression analysis with disease-free survival (DFS) as endpoint] with regard to baseline and tumor characteristics. Second, we evaluated the effect of biomarker positivity on DFS by plotting univariable Kaplan-Meier curves and performing uni- and multivariable Cox regression analyses. Of 102 patients with complete information on p53 status, 44 (43.1%) were p53 positive, and they more often harbored concomitant carcinoma in situ (50.0% vs. 27.6%; P=0.032) and 1973 WHO grade 3 (97.7% vs. 69.0%; P=0.001) compared to their p53 negative counterparts. Of 79

Bloodstream Amyloid-beta (1-40) Peptide, Cognition, and Outcomes in Heart Failure.

Science.gov (United States)

Bayes-Genis, Antoni; Barallat, Jaume; de Antonio, Marta; Domingo, Mar; Zamora, Elisabet; Vila, Joan; Subirana, Isaac; Gastelurrutia, Paloma; Pastor, M Cruz; Januzzi, James L; Lupón, Josep

2017-11-01

In the brain, amyloid-beta generation participates in the pathophysiology of cognitive disorders; in the bloodstream, the role of amyloid-beta is uncertain but may be linked to sterile inflammation and senescence. We explored the relationship between blood levels of amyloid-beta 1-40 peptide (Aβ40), cognition, and mortality (all-cause, cardiovascular, and heart failure [HF]-related) in ambulatory patients with HF. Bloodstream Aβ40 was measured in 939 consecutive patients with HF. Cognition was evaluated with the Pfeiffer questionnaire (adjusted for educational level) at baseline and during follow-up. Multivariate Cox regression analyses and measurements of performance (discrimination, calibration, and reclassification) were used, with competing risk for specific causes of death. Over 5.1 ± 2.9 years, 471 patients died (all-cause): 250 from cardiovascular causes and 131 HF-related. The median Aβ40 concentration was 519.1 pg/mL [Q1-Q3: 361.8-749.9 pg/mL]. The Aβ40 concentration correlated with age, body mass index, renal dysfunction, and New York Heart Association functional class (all P < .001). There were no differences in Aβ40 in patients with and without cognitive impairment at baseline (P = .97) or during follow-up (P = .20). In multivariable analysis, including relevant clinical predictors and N-terminal pro-B-type natriuretic peptide, Aβ40 remained significantly associated with all-cause death (HR, 1.22; 95%CI, 1.10-1.35; P < .001) and cardiovascular death (HR, 1.18; 95%CI, 1.03-1.36; P = .02), but not with HF-related death (HR, 1.13; 95%CI, 0.93-1.37; P = .22). Circulating Aβ40 improved calibration and patient reclassification. Blood levels of Aβ40 are not associated with cognitive decline in HF. Circulating Aβ40 was predictive of mortality and may indicate systemic aging. Copyright © 2017 Sociedad Española de Cardiología. Published by Elsevier España, S.L.U. All rights reserved.

Mechanisms of Nifedipine-Downregulated CD40L/sCD40L Signaling in Collagen Stimulated Human Platelets.

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Tso-Hsiao Chen

Full Text Available The platelet-derived soluble CD40L (sCD40L release plays a critical role in the development of atherosclerosis. Nifedipine, a dihydropyridine-based L-type calcium channel blocker (CCB, has been reported to have an anti-atherosclerotic effect beyond its blood pressure-lowering effect, but the molecular mechanisms remain unclear. The present study was designed to investigate whether nifedipine affects sCD40L release from collagen-stimulated human platelets and to determine the potential role of peroxisome proliferator-activated receptor-β/-γ (PPAR-β/-γ. We found that treatment with nifedipine significantly inhibited the platelet surface CD40L expression and sCD40L release in response to collagen, while the inhibition was markedly reversed by blocking PPAR-β/-γ activity with specific antagonist such as GSK0660 and GW9662. Meanwhile, nifedipine also enhanced nitric oxide (NO and cyclic GMP formation in a PPAR-β/-γ-dependent manner. When the NO/cyclic GMP pathway was suppressed, nifedipine-mediated inhibition of sCD40L release was abolished significantly. Collagen-induced phosphorylation of p38MAPK, ERK1/2 and HSP27, matrix metalloproteinase-2 (MMP-2 expression/activity and reactive oxygen species (ROS formation were significantly inhibited by nifedipine, whereas these alterations were all attenuated by co-treatment with PPAR-β/-γ antagonists. Collectively, these results demonstrate that PPAR-β/-γ-dependent pathways contribute to nifedipine-mediated downregulation of CD40L/sCD40L signaling in activated platelets through regulation of NO/ p38MAPK/ERK1/2/HSP27/MMP-2 signalings and provide a novel mechanism regarding the anti-atherosclerotic effect of nifedipine.

Expression of MDM2 mRNA, MDM2, P53 and P16 Proteins in Urothelial Lesions in the View of the WHO 4th Edition Guidelines as A Molecular Insight towards Personalized Medicine

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Olfat Hammam

2017-08-01

Full Text Available AIM: Here we imposed a multimarker molecular panel composed of P53, MDM2 protein & mRNA & P16 with the identification of sensitive and specific cut offs among the Egyptian urothelial carcinomas bilharzial or not emphasize the pathological and molecular classifications, pathways and prognosis as a privilege for adjuvant therapy. METHODS: Three hundred and ten urothelial lesions were pathologically evaluated and grouped as follows: 50 chronic cystitis as benign, 240 urothelial carcinomas and 20 normal bladder tissue as a control. Immunohistochemistry for MDM Protein, P16 & p53 and In Situ Hybridization for MDM2mRNA were done. RESULTS: MDM2mRNA overexpression correlated with low grade low stage non invasive tumors, while P53 > 40% & p16 40% & P16 10% from high grade, high stage invasive urothelial carcinomas (with p53 > 40, p16 40 & p16 < 10%, together with the histopathological features can distinguish in situ urothelial lesions from dysplastic and atypical lesions.

p53 expression in biopsies from children with Langerhans cell histiocytosis

DEFF Research Database (Denmark)

Bank, Micha I; Lundegaard, Pia Rengtved; Carstensen, Henrik

2002-01-01

based on CD1a positivity. The slides were stained with p53 antibody and semiquantitatively evaluated using a grading system from 1 to 5 as an estimate for 0% to 20%, 20% to 40%, 40% to 60%, 60% to 80%, and 80% to 100% p53-positive for pathologic Langerhans cells (pLC), respectively. RESULTS: The p53...... protein was expressed in various degrees in pLC in all lesions. The degree of p53 expression could not be correlated to either clinical manifestation or outcome. CONCLUSIONS: An increased expression of p53 in pLC indicates an altered DNA repair control with or without abnormal control of apoptosis....

Plasma YKL-40 levels are elevated in patients with chronic heart failure

DEFF Research Database (Denmark)

Rathcke, C.N.; Kistorp, C.; Raymond, I.

2010-01-01

. Median YKL-40 levels were approximately 77% higher in patients with CHF (106 (IQR, 66-184) ng/ml vs. 60 (IQR, 42-97) ng/ml, p creatinine ratio (UACR) (beta = 0.12, p = 0.08). YKL-40 levels were not predictive...

Assessing operational, pricing, and intelligent transportation system strategies for the I-40 corridor using DYNASMART-P.

Science.gov (United States)

2011-09-21

The Raleigh Durham area in North Carolina experienced tremendous growth in both population (50%) and travel (56%) in the : decade spanning 1995-2005. The I-40 corridor, which encompasses I-40, I-85, I-440, I-540, NC147, and US-70 is under great : str...

Air pollution by c-PAHs and plasma levels of p53 and p21WAF1 proteins

Czech Academy of Sciences Publication Activity Database

Rössner ml., Pavel; Binková, Blanka; Milcová, Alena; Solanský, I.; Židzik, J.; Lyubomirova, K.; Farmer, P. B.; Šrám, Radim

2007-01-01

Roč. 620, - (2007), s. 34-40 ISSN 0027-5107 R&D Projects: GA MŽP SI/340/2/00; GA MŽP SL/740/5/03 Grant - others:EU(GB) 2000 -00091 Institutional research plan: CEZ:AV0Z50390512 Source of funding: R - rámcový projekt EK Keywords : air pollution * p53 and p21WAF1 plasma levels Subject RIV: DN - Health Impact of the Environment Quality Impact factor: 4.159, year: 2007

Survival of Lactobacillus plantarumU40 on the in vitro rumen fermentation quantified with real-time PCR

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W.D. Astuti

2018-05-01

Full Text Available The objective of this study was to evaluate the survival of L. plantarumU40 quantified with real-time PCR during in vitro rumen fermentation. The experiment was arranged in a randomized block design with 3 treatments and 4 replications. Treatments were control, rumen fermentation inoculated with L. plantarumU40and L. plantarumU40 + glucose solution. Population of L. plantarum U40 was higher at inoculation treatment. After 8 hours incubation, glucose addition tended to decrease L. plantarum U40 population. Control treatment showed lowest population of L. plantarum U40 along in vitro fermentation compared with other treatment. Inoculation of L. plantarumU40 significantly (p<0.05 increased population of LAB until 12 hours incubation compared with control. Control treatment had highest pH at all incubation time. Glucose addition significantly (P<0.05 decreased final rumen pH (24 hours (6.30, compared with control treatment (6.85. Inoculation of L. plantarum U40 with glucose addition significantly (P<0.05increased propionic acid, decreased acetic acid and A/P ratio compared with other treatments. Lactobacillus plantarum U40 without glucose addition did not affect propionic acid production significantly. As conclusion, Lactobacillus plantarum U40 can survive in rumen fluid and changes rumen fermentation when glucose is added as carbon source.

Ajaleht Pravda ja 40 keisri hullu : "40 kiri" sündis siis, kui teisiti enam ei saanud / Lauri Vahtre

Index Scriptorium Estoniae

Vahtre, Lauri, 1960-

2005-01-01

Eesti kultuuritegelaste kirjutatud "40 kirjast" 1980. aastal, kus tunti muret Eesti ühiskonna ja kultuuri tuleviku pärast. Artikkel sisaldab kirjale allakirjutanute nimesid. Ilmunud ka rmt.: Vahtre, Lauri. Ajaloo pööripäevad. Tallinn, 2006, lk. 190-194

Cyclosporine-resistant, Rab27a-independent Mobilization of Intracellular Preformed CD40L Mediates Antigen-specific T Cell Help In Vitro

Science.gov (United States)

Koguchi, Yoshinobu; Gardell, Jennifer L.; Thauland, Timothy J.; Parker, David C.

2011-01-01

CD40L is critically important for the initiation and maintenance of adaptive immune responses. It is generally thought that CD40L expression in CD4+ T cells is regulated transcriptionally and made from new mRNA following antigen recognition. However, recent studies with two-photon microscopy revealed that the majority of cognate interactions between effector CD4+ T cells and APCs are too short for de novo synthesis of CD40L. Given that effector and memory CD4+ T cells store preformed CD40L (pCD40L) in lysosomal compartments and that pCD40L comes to the cell surface within minutes of antigenic stimulation, we and others have proposed that pCD40L might mediate T cell-dependent activation of cognate APCs during brief encounters in vivo. However, it has not been shown that this relatively small amount of pCD40L is sufficient to activate APCs, owing to the difficulty of separating the effects of pCD40L from those of de novo CD40L and other cytokines in vitro. Here we show that pCD40L surface mobilization is resistant to cyclosporine or FK506 treatment, while de novo CD40L and cytokine expression are completely inhibited. These drugs thus provide a tool to dissect the role of pCD40L in APC activation. We find that pCD40L mediates selective activation of cognate but not bystander APCs in vitro and that mobilization of pCD40L does not depend on Rab27a, which is required for mobilization of lytic granules. Therefore, effector CD4+ T cells deliver pCD40L specifically to APCs on the same time scale as the lethal hit of CTLs but with distinct molecular machinery. PMID:21677130

Sphingosine 1-phosphate (S1P) suppresses the collagen-induced activation of human platelets via S1P4 receptor.

Science.gov (United States)

Onuma, Takashi; Tanabe, Kumiko; Kito, Yuko; Tsujimoto, Masanori; Uematsu, Kodai; Enomoto, Yukiko; Matsushima-Nishiwaki, Rie; Doi, Tomoaki; Nagase, Kiyoshi; Akamatsu, Shigeru; Tokuda, Haruhiko; Ogura, Shinji; Iwama, Toru; Kozawa, Osamu; Iida, Hiroki

2017-08-01

Sphingosine 1-phosphate (S1P) is as an extracellular factor that acts as a potent lipid mediator by binding to specific receptors, S1P receptors (S1PRs). However, the precise role of S1P in human platelets that express S1PRs has not yet been fully clarified. We previously reported that heat shock protein 27 (HSP27) is released from human platelets accompanied by its phosphorylation stimulated by collagen. In the present study, we investigated the effect of S1P on the collagen-induced platelet activation. S1P pretreatment markedly attenuated the collagen-induced aggregation. Co-stimulation with S1P and collagen suppressed collagen-induced platelet activation, but the effect was weaker than that of S1P-pretreatment. The collagen-stimulated secretion of platelet-derived growth factor (PDGF)-AB and the soluble CD40 ligand (sCD40L) release were significantly reduced by S1P. In addition, S1P suppressed the collagen-induced release of HSP27 as well as the phosphorylation of HSP27. S1P significantly suppressed the collagen-induced phosphorylation of p38 mitogen-activated protein kinase. S1P increased the levels of GTP-bound Gαi and GTP-bound Gα13 coupled to S1PPR1 and/or S1PR4. CYM50260, a selective S1PR4 agonist, but not SEW2871, a selective S1PR1 agonist, suppressed the collagen-stimulated platelet aggregation, PDGF-AB secretion and sCD40L release. In addition, CYM50260 reduced the release of phosphorylated-HSP27 by collagen as well as the phosphorylation of HSP27. The selective S1PR4 antagonist CYM50358, which failed to affect collagen-induced HSP27 phosphorylation, reversed the S1P-induced attenuation of HSP27 phosphorylation by collagen. These results strongly suggest that S1P inhibits the collagen-induced human platelet activation through S1PR4 but not S1PR1. Copyright © 2017 Elsevier Ltd. All rights reserved.

A Cytochrome P450 3A4 Biosensor Based on Generation 4.0 PAMAM Dendrimers for the Detection of Caffeine

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Michael Müller

2016-08-01

Full Text Available Cytochromes P450 (CYP, P450 are a large family of heme-active-site proteins involved in many catalytic processes, including steroidogenesis. In humans, four primary enzymes are involved in the metabolism of almost all xenobiotics. Among these enzymes, CYP3A4 is responsible for the inactivation of the majority of used drugs which makes this enzyme an interesting target for many fields of research, especially pharmaceutical research. Since the late 1970s, attempts have been made to construct and develop electrochemical sensors for the determination of substrates. This paper is concerned with the establishment of such a CYP3A4-containing biosensor. The sensor was constructed by adsorption of alternating layers of sub-nanometer gold particle-modified PAMAM (poly-amido-amine dendrimers of generation 4.0, along with the enzyme by a layer-by-layer assembly technique. Atomic force microscopy (AFM, quartz crystal microbalance (QCM, and Fourier-transformed infrared spectroscopy (FTIR were employed to elucidate the sensor assembly. Additionally, the biosensor was tested by cyclic voltammetry using caffeine as a substrate.

40 CFR 426.55 - Standards of performance for new sources.

Science.gov (United States)

2010-07-01

... establish the quantity or quality of pollutants or pollutant properties, controlled by this section, which...— Metric units (g/kkg of product) TSS 0.70 0.70 Oil 1.40 1 .40 Phosphorus 0.05 .05 pH (1) (1) English units (lb/ton of product) TSS 0.0014 0.0014 Oil 0.0028 .0028 Phosphorus 0.0001 .0001 pH (1) (1) 1 Within the...

(p,p') studies of spin excitations in nuclei and of J = 4 nuclear states: Technical progress report

International Nuclear Information System (INIS)

Baker, T.

1989-01-01

During 1988 three experiments were performed. These were: 1 H, 12 C(/rvec n/,p), E/sub n/ = 860 MeV, at SATURNE, 40 Ca(/rvec p/,/rvec p/'), E/sub p/ = 581 MeV, at LAMPF, and 208 Pb(/rvec p/,/rvec p/'), E/sub p/ = 200 MeV at TRIUMF. The purpose of the (n,p) experiment, performed in June and July, was to attempt to corroborate the previously observed shifts in the ( 3 He,t) quasielastic and Δ peaks. The data are being analyzed, and since there was virtually no on-line readout during this experiment, the results are as yet unknown. 4 refs., 4 figs

(p,t) strengths in the Ga isotopes

International Nuclear Information System (INIS)

Rotbard, G.; Vergnes, M.; Berrier-Ronsin, G.; Vernotte, J.; Tamisier, R.

1984-01-01

The (p,t) reaction on the two stable Ga isotopes has been performed at 25 MeV, with 11 keV energy resolution. Levels up to 3.5 MeV in 67 Ga, 4.3 MeV in 69 Ga were measured. Differences observed in comparing the distribution of the L=2 (p,t) strength with the distribution of the B(E2) strength may be explained by a mixed character of the 7/2 2 - level wave function. The distribution of the L=0 strength indicates that the striking change in ground-state structure shown between N=40 and 42 already begins, although weakly, between N=38 and 40

Identification and expression analysis of two interleukin-23α (p19) isoforms, in rainbow trout Oncorhynchus mykiss and Atlantic salmon Salmo salar.

Science.gov (United States)

Jiang, Yousheng; Husain, Mansourah; Qi, Zhitao; Bird, Steve; Wang, Tiehui

2015-08-01

Interleukin (IL)-23 is a heterodimeric IL-12 family cytokine composed of a p19 α-chain, linked to a p40 β-chain that is shared with IL-12. IL-23 is distinguished functionally from IL-12 by its ability to induce the production of IL-17, and differentiation of Th17 cells in mammals. Three isoforms of p40 (p40a, p40b and p40c) have been found in some 3R teleosts. Salmonids also possess three p40 isoforms (p40b1, p40b2 and p40c) although p40a is missing, and two copies (paralogues) of p40b are present that have presumably been retained following the 4R duplication in this fish lineage. Teleost p19 has been discovered recently in zebrafish, but to date there is limited information on expression and modulation of this molecule. In this report we have cloned two p19 paralogues (p19a and p19b) in salmonids, suggesting that a salmonid can possess six potential IL-23 isoforms. Whilst Atlantic salmon has two active p19 genes, the rainbow trout p19b gene may have been pseudogenized. The salmonid p19 translations share moderate identities (22.8-29.9%) to zebrafish and mammalian p19 molecules, but their identity was supported by structural features, a conserved 4 exon/3 intron gene organisation, and phylogenetic tree analysis. The active salmonid p19 genes are highly expressed in blood and gonad. Bacterial (Yersinia ruckeri) and viral infection in rainbow trout induces the expression of p19a, suggesting pathogen-specific induction of IL-23 isoforms. Trout p19a expression was also induced by PAMPs (poly IC and peptidoglycan) and the proinflammatory cytokine IL-1β in primary head kidney macrophages. These data may indicate diverse functional roles of trout IL-23 isoforms in regulating the immune response in fish. Copyright © 2015 Elsevier Ltd. All rights reserved.

Properties of longitudinal and tpansvepse momentum characteristics of charged particles relative to the event principal axis in π+(π-)p interactions at 8 and 40 GeV/c

International Nuclear Information System (INIS)

Sartke, E.; Kanarek, T.; Koval'skj, M.; Solomin, A.N.; Kvatadze, R.A.; Shcheglova, L.M.

1983-01-01

Alterations in longitudinal and transverse momentum characteristics of charged secondaries produced in π + (π - )p interactions at 8 and 40 GeV/c are analyzed when the thrust direction of event impulse configuration is taken as the longitudinal aXis of the center-of-mass system. The usefullness of such frame is discussed

Design and OAM&P aspects of a DWDM system equipped with a 40Gb/s PM-QPSK alien wavelength and adjacent 10Gb/s channels

DEFF Research Database (Denmark)

Nuijts, Roeland; Bjørn, Lars Lange; Petersen, Martin Nordal

2011-01-01

We present theoretical and experimental investigations of the interaction, in terms of BER performance, between a 40Gb/s PM-QPSK alien wavelength and adjacent (50GHz spacing) 10Gb/s NRZ-OOK channels. Experiments were conducted on the Hamburg-Copenhagen section of the Amsterdam-Copenhagen CBF (Cross...... Border Fiber) connection between SURFnet and NORDUnet. Furthermore, we investigated the OAM&P (Operation, Administration, Maintenance and Provisioning) of an alien wavelength in CBF transmission systems....

Usefulness of Plasma YKL-40 in Management of Community-Acquired Pneumonia Severity in Patients

Directory of Open Access Journals (Sweden)

Hsiang-Ling Wang

2013-11-01

Full Text Available Plasma YKL-40 level has been reported as playing a significant role in community-acquired pneumonia (CAP. However, the correlation between plasma level of YKL-40 and the severity of CAP has not been reported. This study identifies the relationship between plasma level changes of the YKL-40 gene in adult patients hospitalized with CAP. The ELISA was used to measure the plasma YKL-40 level from 61 adult CAP patients before and after antibiotic treatment and from 60 healthy controls. The plasma YKL-40 levels were significantly increased in patients with CAP compared to normal controls. Moreover, the plasma concentration of YKL-40 correlated with the severity of CAP based on the pneumonia severity index (PSI score (r = 0.630, p < 0.001, the CURB-65 (confusion, uremia, respiratory rate, BP, age 65 years score (r = 0.640, p < 0.001, the Acute Physiology And Chronic Health Evaluation II (APACHE II score (r = 0.539, p < 0.001 and length of hospital stay (r = 0.321, p = 0.011, respectively. In conclusion, plasma YKL-40 may play a role in the diagnosis and clinical assessment of CAP severity, which could potentially guide the development of treatment strategies.

High YKL-40 serum concentration is correlated with prognosis of Chinese patients with breast cancer.

Directory of Open Access Journals (Sweden)

Dong Wang

Full Text Available This study aimed to investigate the association between serum YKL-40 and prognosis of breast cancer in a Chinese population. Expression of YKL-40 of 120 Chinese patients with breast cancer and 30 controls (benign breast lesions was measured in tumor tissue by immunohistochemistry and in serum by ELISA. Differences in YKL-40 positivity grouped by specific patients' characteristics were compared using Pearson Chi-square test for rates of intratumoral staining, one-way ANOVA with a Bonferroni post-hoc comparison, or two-sample t-test for mean YKL-40 serum concentrations. Factors associated with overall survival were identified by univariate and multivariate cox-regression analyses. YKL-40 was elevated in approximately 75% of Chinese patients with breast cancer. A significantly higher percentage of patients with YKL-40 positive tumors had larger tumor size, higher TNM stage, and/or lymph node metastasis. Significantly higher mean YKL-40 serum concentrations were observed in patient subgroups with invasive lobular carcinoma (P<0.0167, higher TNM stage (P<0.001, and positive lymph node metastasis (P<0.001. The estimated mean survival time of patients with YKL-40 positive tumors was significantly shorter than for patients with YKL-40 negative tumors (55.13 months vs 65.78 months, P = 0.017. Multivariable Cox-regression analysis identified a significant association of overall survival time with YKL-40 serum concentration. Patients with YKL-40 positive tumors had significantly shorter disease free survival times than those with YKL-40 negative tumors. We propose that the potential utility of YKL-40 intratumoral staining or serum concentration as a biomarker for breast cancer is greatest within 5 years of diagnosis.

Plasma YKL-40 in Inuit and Danes

DEFF Research Database (Denmark)

Nøjgaard, Camilla; Johansen, Julia S; Bjerregaard, Peter

2015-01-01

AIMS: The aim of the present study was to investigate whether there are differences in plasma levels of YKL-40 between Inuit in Greenland and in Denmark and in Danes, as well as to study the relationship between alcohol intake, plasma YKL-40 and other factors in Inuit. METHODS: Plasma YKL-40 levels...... were measured on 1645 people from The Greenland Population Study (a cross-sectional population study of Inuit from Denmark and West Greenland) and were compared with the plasma YKL-40 levels of 8899 people from The Copenhagen City Heart Study (a population-based, prospective study of the Danish general...... population). RESULTS: The plasma concentrations of YKL-40 were significantly (P = 0.001) lower in Inuit living in Greenland (median 46 µg/l, range 10-2164, n = 1164) compared with the plasma YKL-40 levels of Inuit living in Denmark (median 63 µg/l, range 20-2827, n = 481) and of Danes living in Denmark...

High serum levels of YKL-40 in patients with squamous cell carcinoma of the head and neck are associated with short survival

DEFF Research Database (Denmark)

Roslind, A.; Johansen, J.S.; Christensen, L.J.

2008-01-01

by immunohistochemistry in 50 patients. Pretreatment serum YKL-40 was elevated in 53%. Patients with high serum YKL-40 had shorter survival than patients with normal serum YKL-40 (33 vs. 84 months; p = 0.008). Multivariate Cox analysis including pretreatment serum YKL-40, age, sex, primary tumor site, TNM classification...... and treatment demonstrated that TNM classification (HR = 2.61, p = 0.02) and serum YKL-40 (log-transformed continuous variable: HR = 1.55, p classification (HR = 5.77, p = 0.001) and serum...... YKL-40 (dichotomous variable: HR = 2.75, p = 0.01) were independent predictors of recurrence-free survival. During follow-up after radiotherapy, a high serum YKL-40 (log-transformed continuous variable) in patients with TNM Stage III and TV disease predicted poorer OS within 6 months (HR = 1.95, p

GaAsP solar cells on GaP/Si with low threading dislocation density

International Nuclear Information System (INIS)

Yaung, Kevin Nay; Vaisman, Michelle; Lang, Jordan; Lee, Minjoo Larry

2016-01-01

GaAsP on Si tandem cells represent a promising path towards achieving high efficiency while leveraging the Si solar knowledge base and low-cost infrastructure. However, dislocation densities exceeding 10"8 cm"−"2 in GaAsP cells on Si have historically hampered the efficiency of such approaches. Here, we report the achievement of low threading dislocation density values of 4.0–4.6 × 10"6 cm"−"2 in GaAsP solar cells on GaP/Si, comparable with more established metamorphic solar cells on GaAs. Our GaAsP solar cells on GaP/Si exhibit high open-circuit voltage and quantum efficiency, allowing them to significantly surpass the power conversion efficiency of previous devices. The results in this work show a realistic path towards dual-junction GaAsP on Si cells with efficiencies exceeding 30%.

Lifetime and spin measurements in 40Ar

International Nuclear Information System (INIS)

Southon, J.

1976-01-01

Lifetimes of levels in 40 Ar populated by the 40 Ar(p,p') reaction have been measured using the Doppler shift attenuation method with a p-γ coincidence technique. A solid argon target was used. The lifetimes determined were (in psec.): 1461 keV level, 1.95 +- 0.15; 2121 keV, >25; 2524 keV, 0.53 +- 0.06; 2893 keV, 4.4 [+2.6,-1.3]; 3208 keV, 0.27. A comprehensive set of branching ratios was also derived and the spins and parities of the 3208 and 4481 keV states were determined to be 2 + and 1 +- respectively. Some of these results suggest that 2 particle -2 hole and 4 particle - 4 hole components are strongly mixed in the low-lying positive parity states in a manner similar to the 2 particle and 4 particle - 2 hole mixing that occurs in 42 Ca. An additional lifetime measurement for the recently discovered high spin state at 3464 keV was carried out using direct electronic timing. The level was excited by the 37 Cl(α,p) reaction and was found to have a lifetime of 1.00 +- 0.03 nsec, which taken together with other evidence indicates that its spin and parity are 6 + . The E2 transition strengths of the 40 Ar 6 + - 4 + - 2 + - 0 + cascade can be simply interpreted in terms of a weak coupling model. (author)

Resilience and recovery of Dehalococcoides mccartyi following low pH exposure.

Science.gov (United States)

Yang, Yi; Cápiro, Natalie L; Yan, Jun; Marcet, Tyler F; Pennell, Kurt D; Löffler, Frank E

2017-12-01

Bioremediation treatment (e.g. biostimulation) can decrease groundwater pH with consequences for Dehalococcoides mccartyi (Dhc) reductive dechlorination activity. To explore the pH resilience of Dhc, the Dhc-containing consortium BDI was exposed to pH 5.5 for up to 40 days. Following 8- and 16-day exposure periods to pH 5.5, dechlorination activity and growth recovered when returned to pH 7.2; however, the ability of the culture to dechlorinate vinyl chloride (VC) to ethene was impaired (i.e. decreased rate of VC transformation). Dhc cells exposed to pH 5.5 for 40 days did not recover the ethene-producing phenotype upon transfer to pH 7.2 even after 200 days of incubation. When returned to pH 7.2 conditions after an 8-, a 16- and a 40-day low pH exposure, tceA and vcrA genes showed distinct fold increases, suggesting Dhc strain-specific responses to low pH exposure. Furthermore, a survey of Dhc biomarker genes in groundwater samples revealed the average abundances of Dhc 16S rRNA, tceA and vcrA genes in pH 4.5-6 groundwater were significantly lower (P-value pH 6-8.3 groundwater. Overall, the results of the laboratory study and the assessment of field data demonstrate that sustained Dhc activity should not be expected in low pH groundwater, and the duration of low pH exposure affects the ability of Dhc to recover activity at circumneutral pH. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Photonucleon reactions in 40Ca at intermediate energies

International Nuclear Information System (INIS)

Adler, J.-O.; Bulow, B.; Jonsson, G.G.; Lindgren, K.

1976-01-01

The yields of the reactions 40 Ca(γ,n) 39 Ca* and 40 Ca(γ,p) 39 K* to the first three excited states have been measured for bremsstrahlung with end-point energies in the region 100-750 MeV. The C 2 S values for the first excited state were deduced from the pion photoproduction contribution to the measured yields. (Auth.)

Coupled-channel analysis of nucleon scattering from 40Ca

International Nuclear Information System (INIS)

Delaroche, J.P.; Honore, G.M.; Tornow, W.; Walter, R.L.

1985-05-01

Differential cross sections and analyzing powers for neutron scattering from 40 Ca have been measured at TUNL in the 10-17 MeV energy range. These measurements and other σ(theta) and σsub(T) measurements, as well as available 40 Ca+p data, have been combined together in a coupled-channel analysis in order to trace the properties (energy dependencies, spin-orbit potentials, deformation parameters) of the nucleon + 40 Ca potential up to 80 MeV

Coupled-channel analysis of nucleon scattering from 40Ca

International Nuclear Information System (INIS)

Delaroche, J.-P.; Honore, G.M.; Tornow, W.; Walter, R.L.

1986-01-01

Differential cross sections and analyzing powers for neutron scattering from 40 Ca have been measured at TUNL in the 10-17 MeV energy range. These measurements and other σ(theta) and σsub(T) measurements, as well as available 40 Ca+p data, have been combined together in a coupled-channel analysis in order to trace the properties (energy dependencies, spin-orbit potentials, deformation parameters) of the nucleon + 40 Ca potential up to 80 MeV. (author)

p-Cymene reduces orofacial nociceptive response in mice

Directory of Open Access Journals (Sweden)

Michele F. Santana

2011-12-01

Full Text Available This study investigated the possible antinociceptive effect of p-cymene in different tests of orofacial nociception. The animals (mice were pretreated (i.p. with p-cymene (25, 50, 100 mg/kg, morphine (5 mg/kg, or vehicle (0.2% Tween 80+saline, and were then subsequently administered, subcutaneously into their upper lip: formalin, capsaicin, and glutamate. The nociceptive behavior response was characterized by the time in s that the mice remained rubbing the orofacial region, for a period of 40 min in the formalin test (first phase, 0-6 min; and second phase, 21-40 min, and for 42 and 15 min in the capsaicin and glutamate tests, respectively. To verify the possible opioid involvement in the antinociceptive effects, naloxone (i.p. was administered into the mice 15 min prior to the pretreatment with p-cymene (100 mg/kg. Finally, whether or not the p-cymene evoked any change in motor performance in the Rota-rod test was evaluated. The results showed that the treatment with p-cymene, at all doses, reduced (p<0.001 the nociceptive behavior in all nociception tests. The antinociceptive effect of p-cymene was antagonized by naloxone (1.5 mg/kg. Additionally, mice treated with p-cymene did not show any change in motor performance. In conclusion, p-cymene attenuated orofacial nociception, suggesting an involvement of the opioid system in this effect. Thus, p-cymene might represent an important biomolecule for management and/or treatment of orofacial pain.

Plasma YKL-40 and recovery of left ventricular function after acute myocardial infarction

DEFF Research Database (Denmark)

Hedegaard, A.; Ripa, Maria Sejersten; Johansen, J.S.

2010-01-01

Background: Plasma YKL-40 is increased early in patients with ST-elevation myocardial infarction (STEMI). It is not known whether plasma YKL-40 is related to infarct size and recovery of ventricular function after primary percutaneous coronary intervention (PCI) of STEMI and whether granulocyte...... colony-stimulating factor (G-CSF) therapy influence plasma YKL-40 concentration. Materials and methods: A total of 72 patients (age: 56 +/- 9 years (mean +/- SD), 56 men and 16 women) with STEMI treated with PCI were included in a double-blind, randomized, placebo-controlled trial with subcutaneous G...... to LVEF recovery (p = 0.04) but not infarct size. G-CSF injections increased YKL-40 compared to placebo (p recovery. Conclusion: Plasma YKL-40 was significantly increased in STEMI patients at admission and G-CSF treatment caused a further increase...

Thermally Induced Structural Transformations of Fe40Ni40P14B6 Amorphous Alloy

Czech Academy of Sciences Publication Activity Database

Vasić, M.; Roupcová, Pavla; Pizúrová, Naděžda; Stevanović, S.; Blagojević, V. A.; Žák, Tomáš; Minić, Dragica M.

47A, č. 1 (2016), s. 260-267 ISSN 1073-5623 R&D Projects: GA MŠk 1M0512 Institutional support: RVO:68081723 Keywords : Fe-Ni-P * soft-magnetic properties * metallic glasses * corrosion resistance * supercooled liquid * crystallization * phase * temperature * Mossbauer Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 1.874, year: 2016

The 4p-5d, 6d and 4p-6s, 7s transitions of Mo IX

International Nuclear Information System (INIS)

Khatoon, S.; Chaghtai, M.S.Z.; Rahimullah, K.

1979-01-01

The transitions 4p-5d, 6d and 4p-6s, 7s have been studied for the first time in Mo IX. The authors have identified 42 4p-5d, 36 4p-6d, 22 4p-6s and 22 4p-7s transitions, establishing 16 4p 3 5d, 14 4p 3 6d and all the ten 4p 3 6s, 7s levels of the spectrum concerned. The ionization energy is estimated to be (1 323 700 +- 700)cm -1 or (164.11 +- 0.09)eV. The spectrum was recorded in sliding and open spark discharges with a 5 m grazing incidence spectrograph of Lund University (Sweden) from about 40 A to 440 A. (Auth.)

High serum levels of YKL-40 in patients with squamous cell carcinoma of the head and neck are associated with short survival

DEFF Research Database (Denmark)

Roslind, Anne; Johansen, Julia S; Christensen, Ib J

2008-01-01

by immunohistochemistry in 50 patients. Pretreatment serum YKL-40 was elevated in 53%. Patients with high serum YKL-40 had shorter survival than patients with normal serum YKL-40 (33 vs. 84 months; p = 0.008). Multivariate Cox analysis including pretreatment serum YKL-40, age, sex, primary tumor site, TNM classification...... and treatment demonstrated that TNM classification (HR = 2.61, p = 0.02) and serum YKL-40 (log-transformed continuous variable: HR = 1.55, p...

Meniscus suture repair: minimum 10-year outcomes in patients younger than 40 years compared with patients 40 and older.

Science.gov (United States)

Steadman, J Richard; Matheny, Lauren M; Singleton, Steven B; Johnson, Nicholas S; Rodkey, William G; Crespo, Bernardo; Briggs, Karen K

2015-09-01

Few studies have compared outcomes after meniscus suture repair in patients younger than 40 years versus patients 40 years and older. To document failure rates and long-term outcomes after meniscus suture repair by a single surgeon, using the inside-out technique, at a minimum 10-year follow-up in patients younger than 40 years versus those 40 years and older. Cohort study; Level of evidence, 3. This study included all patients 18 years or older who underwent meniscus suture repair with the inside-out technique by a single surgeon between January 1992 and December 2003. Patients were divided into 2 cohorts according to age: meniscus as repaired in the index surgery. Patients completed a subjective questionnaire at minimum of 10 years after arthroscopy. Outcomes measures included Lysholm, Tegner, and patient satisfaction with outcome. All data were collected prospectively. The surgeon performed 339 meniscus repairs between 1992 and 2003. The study included 181 knees in 178 patients, who had a mean age of 33 years (range, 18-70 years). Cohort 1 contained 136 knees; 16 patients (12%) were lost to follow-up and 47 (35%) underwent a subsequent knee arthroscopy. Cohort 2 contained 45 knees; 2 patients (4.4%) were lost to follow-up, 3 patients had a total knee arthroplasty, and 12 patients (28%) underwent a subsequent knee arthroscopy. In cohort 1, the meniscus repair failure rate was 5.5% (6/110), and in cohort 2 it was 5.3% (2/38) (P = .927). There was no significant difference in failure rate based on which meniscus was repaired (P = .257), concomitant anterior cruciate ligament (ACL) reconstruction (P = .092), or microfracture (P = .674). Average follow-up time for cohort 1 was 16.1 years (range, 10.0-21.9 years), with 82% follow-up (n = 73/89); average follow-up time for cohort 2 was 16.2 years (range, 10.1-21.0 years), with 93% follow-up (n = 28/30). There were no significant differences in outcomes scores after meniscus suture repair based on age cohort or meniscus

Radioactivity monitoring of the P.P

Energy Technology Data Exchange (ETDEWEB)

Joksic, J.; Todorovic, D.; Radenkovic, M. [VINCA Institute of Nuclear Sciences, Radiation and Enviro nmental Protecti on Lab., Belgrade (Serbia and Montenegro)

2006-07-01

The radioactivity monitoring in the 'Nikola Tesla' thermo-electric power plant environment comprised the analysis of soil, waters, flying ash, slag and plants. Soil, plant and water samples were taken in the environment of the 'Tent A', 'Tent B', 'Kolubara' and 'Morava' power plants during the two years period 2003-2004 and analyzed b y standard radiometric methods. Naturally occurring radionuclides 226 Ra, 232 Th, 40 K, 235 U, 238 U, 210 Pb are determined by gamma spectrometry using H.P. Ge detector, relative efficiency of 23% and energy resolution of 1.8 keV (1332.5 keV). Ceied reference materials: soil matrix with 55 Na, 57 Co, 60 Co, 89 Y, 133 Ba and 137 Cs (MIX-OMHSZ, National Office of Measures) and grass with 134 Cs, 137 Cs, 40 K and 90 Sr (IAEA-373) were used for calibration. The gross alpha and gross beta activity measurements are carried out using low-level background alpha-beta proportional gas counter. Radiochemical analyzes of soil samples have shown the naturally occurring radionuclides: 226 Ra 35-60 Bq/kg, 232 Th 35-49 Bq/kg, 40 K 513-711 Bq/kg, 210 Pb 14-69 Bq/kg, 238 U 25-80 Bq/kg and 235 U 1.1-4.5 Bq/kg. The 137 Cs was found in concentration 10-57 Bq/kg. Radiochemical analyzes of plant samples have shown the naturally occurring radionuclides: 226 Ra 0.7-6.1 Bq/kg, 232 Th 1-4.8 Bq/kg, 40 K 392-1132 Bq/kg, 210 Pb 5.6-97 Bq/kg, 238 U 2.3-7.4 Bq/kg and 235 U 0.2-0.7 Bq/kg. The 137 Cs was found in concentration 0.13-2.8 Bq/kg. The water samples were within the range of recommended values for drinking waters in Serbia and Montenegro. Total alpha activity was below the lowest limit of detection and total beta activities were up to 0.35 Bq/l. The results of measured radionuclides in soil, plant and water samples do not show any significant radioactive environmental pollution. (authors)

Radioactivity monitoring of the P.P

International Nuclear Information System (INIS)

Joksic, J.; Todorovic, D.; Radenkovic, M.

2006-01-01

The radioactivity monitoring in the 'Nikola Tesla' thermo-electric power plant environment comprised the analysis of soil, waters, flying ash, slag and plants. Soil, plant and water samples were taken in the environment of the 'Tent A', 'Tent B', 'Kolubara' and 'Morava' power plants during the two years period 2003-2004 and analyzed b y standard radiometric methods. Naturally occurring radionuclides 226 Ra, 232 Th, 40 K, 235 U, 238 U, 210 Pb are determined by gamma spectrometry using H.P. Ge detector, relative efficiency of 23% and energy resolution of 1.8 keV (1332.5 keV). Certified reference materials: soil matrix with 55 Na, 57 Co, 60 Co, 89 Y, 133 Ba and 137 Cs (MIX-OMHSZ, National Office of Measures) and grass with 134 Cs, 137 Cs, 40 K and 90 Sr (IAEA-373) were used for calibration. The gross alpha and gross beta activity measurements are carried out using low-level background alpha-beta proportional gas counter. Radiochemical analyzes of soil samples have shown the naturally occurring radionuclides: 226 Ra 35-60 Bq/kg, 232 Th 35-49 Bq/kg, 40 K 513-711 Bq/kg, 210 Pb 14-69 Bq/kg, 238 U 25-80 Bq/kg and 235 U 1.1-4.5 Bq/kg. The 137 Cs was found in concentration 10-57 Bq/kg. Radiochemical analyzes of plant samples have shown the naturally occurring radionuclides: 226 Ra 0.7-6.1 Bq/kg, 232 Th 1-4.8 Bq/kg, 40 K 392-1132 Bq/kg, 210 Pb 5.6-97 Bq/kg, 238 U 2.3-7.4 Bq/kg and 235 U 0.2-0.7 Bq/kg. The 137 Cs was found in concentration 0.13-2.8 Bq/kg. The water samples were within the range of recommended values for drinking waters in Serbia and Montenegro. Total alpha activity was below the lowest limit of detection and total beta activities were up to 0.35 Bq/l. The results of measured radionuclides in soil, plant and water samples do not show any significant radioactive environmental pollution. (authors)

Temperature and pressure effects on 40Ar-39Ar systematics

International Nuclear Information System (INIS)

Ozima, M.; Kaneoka, I.; Yanagisawa, M.

1979-01-01

The effects of thermal and compressional treatment on 40 Ar- 39 Ar systematics have been investigated on three artificially heated biotite samples (heated for 1 hour at 700 0 C and 860 0 C in air and 700 0 C in vacuum respectively) and uniaxially compressed granite (p=1400 bar) and basalt samples (p=1660 bar). The 40 Ar- 39 Ar results for the disturbed samples are compared with those for undisturbed samples. Except for the vacuum-heating case, the effects of the disturbances may be interpreted as the combined effect of a partial loss of radiogenic 40 Ar from the sample and an incorporation of air Ar into the sample. Common diagnostic effects are (1) reduction of the total fusion age, (2) distortion of the age spectrum and, if the degree of the partial Ar loss is small (3) approximate preservation of the isochron age, and (4) reduction of the intercept value ( 40 Ar/ 36 Ar) in the isochron plot. The features observed in the age spectra of artificially disturbed samples are rather common in geologically disturbed samples, suggesting that the artificial disturbances simulate the effects of geological disturbances on 40 Ar- 39 Ar systematics. (Auth.)

Distinct pattern of p53 mutations in bladder cancer

DEFF Research Database (Denmark)

Spruck, C H; Rideout, W M; Olumi, A F

1993-01-01

A distinct mutational spectrum for the p53 tumor suppressor gene in bladder carcinomas was established in patients with known exposures to cigarette smoke. Single-strand conformational polymorphism analysis of exons 5 through 8 of the p53 gene showed inactivating mutations in 16 of 40 (40%) bladder...... tumors from smokers and 13 of 40 (33%) tumors from lifetime nonsmokers. Overall, 13 of the 50 (26%) total point mutations discovered in this and previous work were G:C-->C:G transversions, a relatively rare mutational type in human tumors. In six tumors, identical AGA (Arg)-->ACA (Thr) point mutations...... double mutations, four of which were tandem mutations on the same allele. No double mutations were found in tumors from nonsmoking patients. None of the mutations in smokers were G:C-->T:A transversions, which would be anticipated for exposure to the suspected cigarette smoke carcinogen 4-aminobiphenyl...

40Ar/39Ar and K-Ar dating of altered glassy volcanic rocks: the Dabi Volcanics, P.N.G

International Nuclear Information System (INIS)

Walker, D.A.; McDougall, I.

1982-01-01

K-Ar and 40 Ar/ 39 Ar ages have been determined for altered submarine tholeiitic and boninite (high-Mg andesite) lavas from the Dabi Volcanics, Cape Vogel Peninsula, Papua New Guinea. 40 Ar/ 39 Ar whole rock total fusion and plateau ages identify a Late Paleocene age for the tholeiitic lavas (58.9 +- 1.1 Ma), and also for the boninitic lavas (58.8 +- 0.8 Ma). Apparent K-Ar ages for the same samples range from 27.2 +- 0.7 to 63.9 +- 4.5 Ma, and young K-Ar ages for glassy boninites are probably due to variable radiogenic 40 Ar( 40 Ar*) loss. These new ages effectively reconcile previously ambiguous age data for the Dabi Volcanics, and indicate contemporaneous tholeiitic and boninitic volcanism occurring in southeast PNG during the Late Paleocene. Smectites, developed as alteration products after glass in oceanic lavas commonly do not retain 39 Ar during or subsequent to irradiation, but in some cases may contain 40 Ar*. The results are discussed. (author)

Evaluation of Macerating Pectinase Enzyme Activity under Various Temperature, pH and Ethanol Regimes

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Andrew G. Reynolds

2018-02-01

Full Text Available The polygalacturonase (PGU, hemicellulase (mannanase and protease enzyme activities in commercial macerating, pectinase-enzyme preparations commonly used by wineries in Ontario (Scottzyme Color X and Color Pro were measured under various simulated process conditions (temperature, pH, and ethanol concentration. Treatments included three temperatures (15, 20 and 30 °C; pH = 3.0, 3.5, 4.0 and 5.0; ethanol = 0%, four pH levels (3.0, 3.5, 4.0 and 5.0; temperature = 15, 20, 30 and 50 °C; ethanol = 0%, and four ethanol concentrations ((2.5, 5, 7.5 and 10%; temperature = 20 °C and pH = 3.5. Polygalacturonase enzyme activity in Color X increased linearly with temperature at all pH levels, and increased with pH at all temperature regimes. Polygalacturonase activity decreased with increasing ethanol. Color X mannanase activity increased with temperatures between 15 and 40 °C, and decreased with increased pH between 3.0 and 5.0. Response of mannanase to ethanol was cubic with a sharp decrease between 8 and 10% ethanol. Protease activity increased linearly with temperatures between 20 and 40 °C. These data suggest that the PGU, mannanase and protease components in these enzyme products provide sufficient activities within the ranges of pH, temperature, and ethanol common during the initial stages of red wine fermentations, although low must temperatures (<20 °C and presence of ethanol would likely lead to sub-optimal enzyme activities.

Impact of 9p deletion and p16, Cyclin D1, and Myc hyperexpression on the outcome of anaplastic oligodendrogliomas.

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Karine Michaud

Full Text Available To study the presence of 9p deletion and p16, cyclin D1 and Myc expression and their respective diagnostic and prognostic interest in oligodendrogliomas.We analyzed a retrospective series of 40 consecutive anaplastic oligodendrogliomas (OIII from a single institution and compared them to a control series of 10 low grade oligodendrogliomas (OII. Automated FISH analysis of chromosome 9p status and immunohistochemistry for p16, cyclin D1 and Myc was performed for all cases and correlated with clinical and histological data, event free survival (EFS and overall survival (OS.Chromosome 9p deletion was observed in 55% of OIII (22/40 but not in OII. Deletion was highly correlated to EFS (median = 29 versus 53 months, p<0.0001 and OS (median = 48 versus 83 months, p<0.0001 in both the total cohort and the OIII population. In 9p non-deleted oligodendrogliomas, p16 hyperexpression correlated with a shorter OS (p = 0.02 in OII and p = 0.0001 in OIII whereas lack of p16 expression was correlated to a shorter EFS and OS in 9p deleted OIII (p = 0.001 and p = 0.0002 respectively. Expression of Cyclin D1 was significantly higher in OIII (median expression 45% versus 14% for OII, p = 0.0006 and was correlated with MIB-1 expression (p<0.0001, vascular proliferation (p = 0.002, tumor necrosis (p = 0.04 and a shorter EFS in the total cohort (p = 0.05. Hyperexpression of Myc was correlated to grade (median expression 27% in OII versus 35% in OIII, p = 0.03, and to a shorter EFS in 9p non-deleted OIII (p = 0.01.Chromosome 9p deletion identifies a subset of OIII with significantly worse prognosis. The combination of 9p status and p16 expression level identifies two distinct OIII populations with divergent prognosis. Hyperexpression of Bcl1 and Myc appears highly linked to anaplasia but the prognostic value is unclear and should be investigated further.

Chromosome number in Brazilian germplasm accessions of Paspalum hydrophilum, P. modestum and P. palustre (Gramineae; Paniceae

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Pozzobon Marisa T.

2003-01-01

Full Text Available This paper compiles results of chromosome counts of Paspalum hydrophilum, P. modestum and P. palustre. Four Brazilian accessions of P. modestum have shown 2n = 2x = 20 chromosomes, a number already found in one accession from Argentina and in two from Brazil. Three other Brazilian accessions showed tetraploid level (2n = 4x = 40, which was previously unknown in this species. In P. hydrophilum, only one of the accessions analyzed presented tetraploid level, initially established for the species from plants collected in Argentina. Five additional accessions from Brazil showed the diploid number, previously detected in a single Brazilian population. A tetraploid cytotype was found in P. palustre, previously known as a diploid species. In addition to confirming the occurrence of distinct ploidy levels for all three species, the results establish the predominance of the diploid level in P. hydrophilum and P. modestum accessions collected in Brazil.

Photon asymmetry from radiative muon capture on 40Ca

International Nuclear Information System (INIS)

Larabee, A.J.; Burnham, R.A.; Gorringe, T.P.; Hasinoff, M.D.; Horvath, D.; Noble, A.J.; Pouladdej, A.; Virtue, C.J.; Azuelos, G.; Robertson, B.C.; Wright, D.H.

1989-01-01

The photon asymmetry (α γ) for 40 Ca has been measured at TRIUMF using the muon spin-rotation technique. From the asymmetry measurement, the value of the pseudoscalar coupling constant, g p , can be obtained. A total of 5500 clean photon events were collected. The preliminary value found for the photon asymmetry of 40 Ca is 1.00 ± 0.23

Coupled-channel analysis of nucleon scattering from /sup 40/Ca

Energy Technology Data Exchange (ETDEWEB)

Delaroche, J.-P.; Honore, G.M.; Tornow, W.; Walter, R.L.

1986-01-01

Differential cross sections and analyzing powers for neutron scattering from /sup 40/Ca have been measured at TUNL in the 10-17 MeV energy range. These measurements and other sigma(theta) and sigmasub(T) measurements, as well as available /sup 40/Ca+p data, have been combined together in a coupled-channel analysis in order to trace the properties (energy dependencies, spin-orbit potentials, deformation parameters) of the nucleon + /sup 40/Ca potential up to 80 MeV.

pSv3neo transfection and radiosensitivity of human cancer cell lines

International Nuclear Information System (INIS)

Parris, C.N.; Masters, J.R.W.; Green, M.H.L.

1990-01-01

Immortalisation of human fibroblasts by transfection with a plasmid, pSV3neo, results in an increase in their radioresistance. The change in radiosensitivity may either be a consequence of transformation or due to expression of the SV40 T-antigen in pSV3neo. To investigate these two possibilities, we transfected pSV3neo into cells already transformed and immortalised. The radiosensitivies of three human bladder cancer cell lines were unaltered in clones expressing T-antigen, indicating that the changes observed in fibroblasts probably are a consequence of transformation, and not the presence of SV40 T-antigen. (author)

SERUM YKL-40 CORRELATES WITH SERUM INTERLEUKIN-6 IN MULTIPLE MYELOMA

DEFF Research Database (Denmark)

Mylin, Anne Kjærsgaard; Andersen, Niels Frost; Johansen, Julia S.

2007-01-01

Introduction. The secreted glycoprotein YKL-40 (CHI3L1, HC gp-39) is a potential player in the tumor-host interactions affecting several aspects of multiple myeloma (MM) including angiogenesis. YKL-40 expression is seeen in vascular smooth muscle cells, and the protein is suggested to function......, but S-YKL-40 show a strong positive correlation with S-IL-6 consistent with a possible IL-6 mediated regulation of YKL-40 secretion demonstrated in previous studies. Table 1     Normal S-YKL-40 N   Median (range)     Elevated S-YKL-40 N   Median (range)     p-value   S-IL-6, ng/L             PB 23 3 (1...

Corrosion studies of thermally sensitised AGR fuel element brace in pH7 and pH9.2 borate solutions

International Nuclear Information System (INIS)

Tyfield, S.P.; Smith, C.A.

1987-04-01

Brace and cladding of AGR fuel elements sensitised in reactor are susceptible to intergranular and crevice corrosion, which may initiate in the pH7 borate pond storage environment of CEGB/SSEB stations. This report considers the benefit in corrosion control that is provided by raising the pond solution pH to 9.2, whilst maintaining the boron level at 1250 gm -3 . The greater corrosion protection provided by pH9.2 solution compared to the pH7 borate solution is demonstrated by a series of tests with non-active laboratory sensitised brace samples exposed to solutions dosed with chloride or sulphate in order to promote localised corrosion. The corrosion tests undertaken consisted of 5000 hour immersions at 32 0 C and shorter term electrochemically monitored experiments (rest potential, impedance, anodic current) generally conducted at 22 0 C. The pH9.2 solution effectively inhibited the initiation of crevice and intergranular corrosion in the presence of low levels of chloride and sulphate, whereas the pH7 solution did not always do so. However, the pH9.2 solution, dosed with 40 gm -3 chloride, failed to suppress fully crevice corrosion initiated in unborated 40 gm -3 chloride solution at 22 0 C. Fluoride is not deleterious at low levels ∼ 10 gm -3 in the borate solutions. The significant improvement in corrosion control demonstrated for the change from pH7 to pH9.2 borate solution on laboratory sensitised brace samples should ideally be confirmed using complete irradiated AGR fuel elements. (U.K.)

Serum YKL-40 is increased in patients with hepatic fibrosis

DEFF Research Database (Denmark)

Johansen, J S; Christoffersen, P; Møller, S

2000-01-01

BACKGROUND/AIMS: YKL-40, a mammalian member of the chitinase family, is a lectin that binds heparin and chitin. The function of YKL-40 is unknown, but it may function in tissue remodelling. The aims of this study were to assess the level of circulating YKL-40 in patients with various kinds...... with the blood sample. RESULTS: The median serum YKL-40 was highest in patients with alcoholic cirrhosis (532 microg/l), in particular in patients with additional alcoholic hepatitis (740 microg/l). Patients with alcoholic cirrhosis, post-hepatitic cirrhosis (425 microg/l) and non-cirrhotic fibrosis (330 microg/l......) had significantly higher serum YKL-40 than normal subjects (102 microg/l), patients with fatty liver (195 microg/l) or patients with viral hepatitis without fibrosis (174 microg/l). Serum YKL-40 was significantly (p

40 CFR 721.4420 - Substituted hydroxylamine.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Substituted hydroxylamine. 721.4420... Substances § 721.4420 Substituted hydroxylamine. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified generically as substituted hydroxylamine (PMN P-84-492) is...

40 CFR 721.8095 - Silylated polyurethane.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Silylated polyurethane. 721.8095... Substances § 721.8095 Silylated polyurethane. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified generically as a silylated polyurethane (PMN P-95-1356) is...

Comparison of the effects of esomeprazole 40 mg, rabeprazole 20 mg, lansoprazole 30 mg, and pantoprazole 40 mg on intragastrıc pH in extensive metabolizer patients with gastroesophageal reflux disease.

Science.gov (United States)

Çelebi, Altay; Aydın, Dinçer; Kocaman, Orhan; Konduk, Buğra Tolga; Şentürk, Ömer; Hülagü, Sadetin

2016-09-01

Studies on the therapeutic efficacy of proton pump inhibitors (PPIs) in patients with gastroesophageal reflux disease (GERD) have been recently published. In most of these studies, comparison of only two PPIs have been made. There are few studies on the comparison of four or more PPIs. We aimed to compare the acid inhibitory effects of esomeprazole 40 mg, rabeprazole 20 mg, lansoprazole 30 mg, and pantoprazole 40 mg on days 1 and 5 of treatment in patients with GERD, who were extensive metabolizers in regard to the CYP2C19 genotype. Helicobacter pylori-negative with typical symptoms of GERD patients were randomly divided into four treatment groups. Efficacy analysis on days 1 and 5 were performed on the four groups which comprised 10 (esomeprazole), 11 (rabeprazole), 10 (lansoprazole), and 10 (pantoprazole) patients. On day 1 of PPI treatment, the mean percentage of time with intragastric Ph>4 were 54%, 58%, 60%, and 35% for the groups, respectively, and on day 5, these values were 67%, 60%, 68%, and 59%, respectively. Esomeprazole, rabeprazole, and lansoprazole were found to be superior to pantoprazole on the first day of treatment. Pantoprazole is a less potent proton pump inhibitor than the other PPIs tested on the first day of treatment. When the time needed to raise the intragatric pH to over 4 was evaluated, esomeprazole was found to have the most rapid action, followed by lansoprazole and rabeprazole.

40 CFR 131.40 - Puerto Rico

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 21 2010-07-01 2010-07-01 false Puerto Rico 131.40 Section 131.40... Federally Promulgated Water Quality Standards § 131.40 Puerto Rico (a) Use designations for marine waters. In addition to the Commonwealth's adopted use designations, the following waterbodies in Puerto Rico...

Glycoprotein YKL-40 Levels in Plasma Are Associated with Fibrotic Changes on HRCT in Asbestos-Exposed Subjects

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Tuija Väänänen

2017-01-01

Full Text Available YKL-40 is a chitinase-like glycoprotein produced by alternatively activated macrophages that are associated with wound healing and fibrosis. Asbestosis is a chronic asbestos-induced lung disease, in which injury of epithelial cells and activation of alveolar macrophages lead to enhanced collagen production and fibrosis. We studied if YKL-40 is related to inflammation, fibrosis, and/or lung function in subjects exposed to asbestosis. Venous blood samples were collected from 85 men with moderate or heavy occupational asbestos exposure and from 28 healthy, age-matched controls. Levels of plasma YKL-40, CRP, IL-6, adipsin, and MMP-9 were measured with enzyme-linked immunosorbent assay (ELISA. Plasma YKL-40 levels were significantly higher in subjects with asbestosis (n=19 than in those with no fibrotic findings in HRCT following asbestos exposure (n=66 or in unexposed healthy controls. In asbestos-exposed subjects, plasma YKL-40 correlated negatively with lung function capacity parameters FVC (Pearson’s r −0.259, p=0.018 and FEV1 (Pearson’s r −0.240, p=0.028 and positively with CRP (Spearman’s rho 0.371, p<0.001, IL-6 (Spearman’s rho 0.314, p=0.003, adipsin (Spearman’s rho 0.459, p<0.001, and MMP-9 (Spearman’s rho 0.243, p=0.025. The present finding suggests YKL-40 as a biomarker associated with fibrosis and inflammation in asbestos-exposed subjects.

Characterization of bacteriophage KVP40 and T4 RNA ligase 2

International Nuclear Information System (INIS)

Yin Shenmin; Kiong Ho, C.; Miller, Eric S.; Shuman, Stewart

2004-01-01

Bacteriophage T4 RNA ligase 2 (Rnl2) exemplifies a subfamily of RNA strand-joining enzymes that includes the trypanosome RNA editing ligases. A homolog of T4 Rnl2 is encoded in the 244-kbp DNA genome of vibriophage KVP40. We show that the 335-amino acid KVP40 Rnl2 is a monomeric protein that catalyzes RNA end-joining through ligase-adenylate and RNA-adenylate (AppRNA) intermediates. In the absence of ATP, pre-adenylated KVP40 Rnl2 reacts with an 18-mer 5'-PO 4 single-strand RNA (pRNA) to form an 18-mer RNA circle. In the presence of ATP, Rnl2 generates predominantly AppRNA. Isolated AppRNA can be circularized by KVP40 Rnl2 in the absence of ATP. The reactivity of phage Rnl2 and the distribution of the products are affected by the length of the pRNA substrate. Whereas 18-mer and 15-mer pRNAs undergo intramolecular sealing by T4 Rnl2 to form monomer circles, a 12-mer pRNA is ligated intermolecularly to form dimers, and a 9-mer pRNA is unreactive. In the presence of ATP, the 15-mer and 12-mer pRNAs are converted to AppRNAs, but the 9-mer pRNA is not. A single 5' deoxynucleotide substitution of an 18-mer pRNA substrate has no apparent effect on the 5' adenylation or circularization reactions of T4 Rnl2. In contrast, a single deoxyribonucleoside at the 3' terminus strongly and selectively suppresses the sealing step, thereby resulting in accumulation of high levels of AppRNA in the absence of ATP. The ATP-dependent 'capping' of RNA with AMP by Rnl2 is reminiscent of the capping of eukaryotic mRNA with GMP by GTP:RNA guanylyltransferase and suggests an evolutionary connection between bacteriophage Rnl2 and eukaryotic RNA capping enzymes

40 CFR 40.115-1 - Construction.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Construction. 40.115-1 Section 40.115-1... DEMONSTRATION GRANTS § 40.115-1 Construction. May include the preliminary planning to determine the economic and... action necessary to the construction of a facility, the erection, acquisition, alteration, remodeling...

Coupled-channel analysis of nucleon scattering from /sup 40/Ca

International Nuclear Information System (INIS)

Delaroche, J.P.; Honore, G.M.; Tornow, W.; Walter, R.L.

1985-01-01

Differential cross sections and analyzing powers for neutron scattering from /sup 40/Ca have been measured at TUNL in the 10-17 MeV energy range. These measurements and other σ(θ) and σ/sub T/ measurements, as well as available /sup 40/Ca+p data, have been combined together in a coupled-channel analysis in order to trace the properties (energy dependencies, spin-orbit potentials, deformation parameters) of the nucleon + /sup 40/Ca potential up to 80 MeV

VLS growth of alternating InAsP/InP heterostructure nanowires for multiple-quantum-dot structures.

Science.gov (United States)

Tateno, Kouta; Zhang, Guoqiang; Gotoh, Hideki; Sogawa, Tetsuomi

2012-06-13

We investigated the Au-assisted growth of alternating InAsP/InP heterostructures in wurtzite InP nanowires on InP(111)B substrates for constructing multiple-quantum-dot structures. Vertical InP nanowires without stacking faults were obtained at a high PH(3)/TMIn mole flow ratio of 300-1000. We found that the growth rate changed largely when approximately 40 min passed. Ten InAsP layers were inserted in the InP nanowire, and it was found that both the InP growth rate and the background As level increased after the As supply. We also grew the same structure using TBAs/TBP and could reduce the As level in the InP segments. A simulation using a finite-difference time-domain method suggests that the nanowire growth was dominated by the diffusion of the reaction species with long residence time on the surface. For TBAs/TBP, when the source gases were changed, the formed surface species showed a short diffusion length so as to reduce the As background after the InAsP growth.

40 CFR 40.115-6 - State.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 1 2010-07-01 2010-07-01 false State. 40.115-6 Section 40.115-6... DEMONSTRATION GRANTS § 40.115-6 State. (a) Under the Federal Water Pollution Control Act, a State, the District... Territory of the Pacific Islands. (b) Under the Resource Conservation and Recovery Act, a State, the...

40 CFR 721.1820 - Bisphenol derivative.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Bisphenol derivative. 721.1820 Section... Substances § 721.1820 Bisphenol derivative. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified generically as bisphenol derivative (PMN No. P-92-509) is...

40 CFR 721.405 - Polyether acrylate.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Polyether acrylate. 721.405 Section... § 721.405 Polyether acrylate. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified generically as a polyether acrylate (PMN P-95-666) is subject to...

40 CFR 721.8090 - Polyurethane polymer.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Polyurethane polymer. 721.8090 Section... Substances § 721.8090 Polyurethane polymer. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified generically as a polyurethane polymer (P-94-47) is subject...

Kinetics of the Reactions of O((sup 3)P) and Cl((sup 2)P) with HBr and Br2

Science.gov (United States)

Nicovich, J. M.; Wine, P. H.

1997-01-01

A laser flash photolysis-resonance fluorescence technique has been employed to study the kinetics of reactions (1)-(4) as a function of temperature. (1) O((sup 3)P) + Br2 yields BrO + Br((sup 2)P(sub 3/2)) at 255-350 K; (2) Cl((sup 2)P) + Br2 yields BrCl + Br((sup 2)P(sub 3/2)) at 298-401 K; (3) O((sup 3)P) + HBr yields OH + Br((sup 2)P(sub J)) at 250-402 K; (4) Cl((sup 2)P) + HBr yields HCl + Br((sup 2)P(sub J)) at 257-404 K. In all cases, the concentration of the excess reagent, i.e, HBr or Br2, was measured in situ in the slow flow system by UV-visible photometry. Heterogeneous dark reactions between XBr (X equals H or Br) and the photolytic precursors for Cl((sup 2)P) and O((sup 3)P) (Cl2 and O3, respectively) were avoided by injecting minimal amounts of precursor into the reaction mixture immediately upstream from the reaction zone. The following Arrhenius expressions summarize our results (errors are 2 sigma and represent precision only, units are cu cm/(molecule.s): k(sub 1) = (1.76 +/- 0.80) x 10(exp -11 exp[(40 +/- 100)/T]; k(sub 2) = (2.40 +/- 1.25) x 12(exp -10) exp[-(144 +/- 176)/T]; k(sub 3) = (5.11 +/- 2.82) x 10(exp -12) exp[-(1450 +/- 160)/T]; k(sub 4) = (2.25 +/- 0.56) x 10(exp -11) exp[-(400 +/- 80)/T]. The consistency (or lack thereof) of our results with those reported in previous kinetics and dynamics studies of reactions (1)-(4) is discussed.

Fibril Formation by pH and Temperature Responsive Silk-Elastin Block Copolymers

NARCIS (Netherlands)

Golinska, M.D.; Pham, T.T.H.; Werten, M.W.T.; Wolf, de F.A.; Cohen Stuart, M.A.; Gucht, van der J.

2013-01-01

In this report, we study the self-assembly of two silk-elastin-like proteins: one is a diblock S24E40 composed of 24 silk-like (S) repeats and 40 elastin-like (E) repeats; the other is a triblock S12C4E40, in which the S and E blocks are separated by a random coil block (C4). Upon lowering the pH,

Serum YKL-40 and gestational diabetes - an observational cohort study

DEFF Research Database (Denmark)

Gybel-Brask, Dorte; Johansen, Julia S; Christiansen, Ib J

2016-01-01

To examine serum YKL-40 in women developing gestational diabetes mellitus (GDM). In the present large observational cohort study of 1179 pregnant women, we determined serum YKL-40 four times during pregnancy (at gestational age 12, 20, 25, and 32 weeks). Pregnancy outcome was obtained from medical...... records. Sixty-eight women (5.8%) developed GDM. Serum YKL-40 increased from gestational age (GA) 12 weeks and the following weeks in the women who developed GDM and was independent of BMI, parity, and maternal age (OR = 2.69, 95% CI: 1.45-5.00, p = 0.002). No association was found between serum YKL-40...

Zγ production in p bar p collisions at √ (s) =1.8TeV and limits on anomalous ZZγ and Zγγ couplings

International Nuclear Information System (INIS)

Gomez, B.; Hoeneisen, B.; Mooney, P.; Negret, J.P.; Davis, K.; Fein, D.; Forden, G.E.; Guida, J.A.; James, E.; Johns, K.; Nang, F.; Narayanan, A.; Rutherfoord, J.; Butler, J.M.; Fatyga, M.; Gibbard, B.; Gordon, H.; Graf, N.; Kahn, S.; Kotcher, J.; Protopopescu, S.; Rajagopalan, S.; Bantly, J.; Cullen-Vidal, D.; Cutts, D.; Guida, J.M.; Hoftun, J.S.; Partridge, R.; Grinstein, S.; Piegaia, R.; Bloom, P.; Fisyak, Y.; Glenn, S.; Grim, G.; Klopfenstein, C.; Lander, R.; Mani, S.; Fahland, T.; Hall, R.E.; Boswell, C.; Choudhary, B.C.; Cochran, J.; Ellison, J.; Gartung, P.; Gounder, K.; Heinson, A.P.; Huehn, T.; Alves, G.A.; Carvalho, W.; Maciel, A.K.; Motta, H.; Santoro, A.; Castilla-Valdez, H.; Gonzalez Solis, J.L.; Hernandez-Montoya, R.; Magana-Mendoza, L.; Sanchez-Hernandez, A.; Adam, I.; Kotwal, A.V.; Bhattacharjee, M.; Ahn, S.; Baldin, B.; Bartlett, J.F.; Bhat, P.C.; Boehnlein, A.; Borcherding, F.; Brandt, A.; Bross, A.; Christenson, J.H.; Cooper, W.E.; Demarteau, M.; Denisov, D.; Diehl, H.T.; Diesburg, M.; Feher, S.; Fisk, H.E.; Flattum, E.; Fuess, S.; Genser, K.; Gerber, C.E.; Green, D.R.; Greenlee, H.; Grossman, N.; Haggerty, H.; Hansen, S.; Heintz, U.; Hobbs, J.D.; Ito, A.S.

1998-01-01

We present a study of Zγ+X production in p bar p collisions at √ (s) =1.8TeV from 97 (87)pb -1 of data collected in the eeγ (μmuγ) decay channel with the D0 detector at Fermilab. The event yield and kinematic characteristics are consistent with the standard model predictions. We obtain limits on anomalous ZZγ and Zγγ couplings for form factor scales Λ=500GeV and Λ=750GeV. Combining this analysis with our previous results yields 95% C.L. limits [h 30 Z ] 40 Z ] 30 γ ] 40 γ ]<0.05 for a form factor scale Λ=750GeV. copyright 1998 The American Physical Society

Tumores malignos de pálpebra Malignant eyelid tumors

Directory of Open Access Journals (Sweden)

Luis Henrique Schneider Soares

2001-08-01

Full Text Available Objetivos: Estudar a incidência de tumores malignos de pálpebra no Hospital Banco de Olhos de Porto Alegre. Métodos: Estudo retrospectivo dos casos de tumores malignos de pálpebra no período de 1985 a 1997, que tiveram diagnóstico confirmado por exame anátomopatológico. Resultados: Foram encontradas 54 neoplasias malignas, sendo 75,92% carcinoma basocelular, 12,96% carcinoma espinocelular, 7,40% melanoma e 1,85% lentigo maligna. A maioria dos pacientes apresentava mais de 40 anos e não houve prevalência de sexo. Conclusões: O tumor de pálpebra mais freqüente em nosso meio foi o carcinoma basocelular, seguido do carcinoma espinocelular. O melanoma foi o terceiro em freqüência mais encontrado em nossa pesquisa.Purposes: To study the incidence of eyelid malignant tumors in the Banco de Olhos Hospital of Porto Alegre from 1985 to 1997. Methods: We retrospectivelly analyzed clinical archives and in this study all cases of malignant eyelid tumors with histopathologic examination were included. Results: We found 54 eyelid tumors: 75.92% basal cell, 12.96% squamous cell, 7.40% melanoma, 1.85% undifferentiated carcinoma and 1.85% lentigo maligna. The majority of the patients was over 40 years old, 50% were male and 50% female. The diagnosis was confirmed in all cases through histopathologic examination. Conclusions: Basal cell carcinoma was the most frequent eyelid malignancy followed by squamous cell carcinoma. Melanoma was the third most frequently found tumor in our study.

40 CFR 133.102 - Secondary treatment.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 21 2010-07-01 2010-07-01 false Secondary treatment. 133.102 Section... TREATMENT REGULATION § 133.102 Secondary treatment. The following paragraphs describe the minimum level of effluent quality attainable by secondary treatment in terms of the parameters—BOD5, SS and pH. All...

40 CFR 721.9892 - Alkylated urea.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Alkylated urea. 721.9892 Section 721... Alkylated urea. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified generically as an alkylated urea (PMN P-93-1649) is subject to reporting under this...

40 CFR 721.9928 - Urea, tetraethyl-.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Urea, tetraethyl-. 721.9928 Section... Substances § 721.9928 Urea, tetraethyl-. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified as urea, tetraethyl- (PMN P-94-1017; CAS No. 1187-03-7) is...

IL-23 p19 knockout mice exhibit minimal defects in responses to primary and secondary infection with Francisella tularensis LVS.

Directory of Open Access Journals (Sweden)

Sherry L Kurtz

Full Text Available Our laboratory's investigations into mechanisms of protective immunity against Francisella tularensis Live Vaccine Strain (LVS have uncovered mediators important in host defense against primary infection, as well as those correlated with successful vaccination. One such potential correlate was IL-12p40, a pleiotropic cytokine that promotes Th1 T cell function as part of IL-12p70. LVS-infected IL-12p40 deficient knockout (KO mice maintain a chronic infection, but IL-12p35 KO mice clear LVS infection; thus the role that IL-12p40 plays in immunity to LVS is independent of the IL-12p70 heterodimer. IL-12p40 can also partner with IL-23p19 to create the heterodimeric cytokine IL-23. Here, we directly tested the role of IL-23 in LVS resistance, and found IL-23 to be largely dispensable for immunity to LVS following intradermal or intranasal infection. IL-23p19 KO splenocytes were fully competent in controlling intramacrophage LVS replication in an in vitro overlay assay. Further, antibody responses in IL-23p19 KO mice were similar to those of normal wild type mice after LVS infection. IL-23p19 KO mice or normal wild type mice that survived primary LVS infection survived maximal doses of LVS secondary challenge. Thus p40 has a novel role in clearance of LVS infection that is unrelated to either IL-12 or IL-23.

Limits on the anomalous ZZγ and Zγγ couplings in p anti p collisions at √s = 1.8 TeV

International Nuclear Information System (INIS)

Abachi, S.

1995-07-01

The authors performed a direct search for the anomalous ZZγ and Zγγ couplings by studying p anti p → ell ell γ + X, (ell = e, μ) events at √s = 1.8 TeV with the D0 detector at the Fermilab Tevatron Collider. A fit to the transverse energy spectrum of the photon in the signal events, based on the data set corresponding to an integrated luminosity of 14.3 pb -1 (13.7 pb -1 ) for the electron (muon) channel, yields the following 95% confidence level limits on the anomalous CP-conserving ZZγ couplings: |h 30 Z | 40 Z = 0), and |h 40 Z | 30 Z = 0), for a form-factor scale Λ = 500 GeV. Limits for the Zγγ couplings and CP-violating couplings are also discussed

Project installation the large equipment in line system in Brazil. Gas export line valve P-40 FPSO-MLS. Field Marlim Sul, Campos Basin, Brazil; Operacao de instalacao do maior equipamento no sistema in line ja realizado no Brasil. Valvula do gasoduto P-40 X FPSO-MLS. Campo de Marlim Sul, Bacia de Campos, Brasil

Energy Technology Data Exchange (ETDEWEB)

Marques, Marcos Antonio Rodrigues; Fernandes, Paulo Tavares [PETROBRAS, Campos dos Goytacases, RJ (Brazil). Exploracao e Producao

2005-07-01

This work will approach the current level of development of the installation of connected underwater equipment to flexible lines in the underwater engineering operations in Campos' Basin. The project will show studies, analysis and simulations (through software developed by PETROBRAS) about the installation of the largest equipment laid in the 'in-line' system (connected to flexible lines) in Brazil - and one of the largest of the world: the ESDV (Emergency Shut Down Valve) of the gas pipeline P-40 x FPSO-MLS, in the South Marlim field, in Campos' Basin. This ESDV, of about 18.000 kg, 4 m height and 6,5 m length, has the purpose of assuring the safety conditions on the facilities, interrupting the gas flow exported for P-40 in case of emergency situations. Its installation opened a new alternative in releasing underwater equipment, using the ships that install the flexible lines. This operation occurred in June, 2004, and required the use of a second vessel for support and monitoring of the ESDV laying. The ESDV was installed at 400 m from FPSO-MLS, in a water depth of 1.137 m. This method shall be used broadly by the company in the implantation of the new units of Campos' Basin, and the upcoming studies must consider the gradual increase of the water depth in the new projects. This work will focus the technological development in this area, and one of its purposes is to foresee the future difficulties that can appear in the implantation of the production systems in deep and ultra-deep waters. (author)

Avaliação de refluxo ultra-distal com pHmetria de múltiplos canais Ultra-distal reflux evaluation with multiple channel pHmonitoring

Directory of Open Access Journals (Sweden)

Francisco Carlos Bernal da Costa Seguro

2011-03-01

, intragastric. The DeMeester score was assessed on channel A. The number of episodes of acid reflux, the number of episodes of prolonged reflux and fraction of time pH<4.0 were compared on channels A and B. The fraction of time pH<4.0 was compared on channels B and C. The fraction of time with pH<4.0 above 50% on channel D was used as parameter of no proximal migration of the catheter. RESULTS: Significant increase in the number of reflux episodes and fraction of time pH<4.0 in channel B in relation to channel A. Reduced fraction of time pH<4.0 in channel B compared to channel C was seen. Two cases of esophageal adenocarcinoma were diagnosed in the group. CONCLUSIONS: The zone 1 cm above the top edge of the LES is more exposed to acidity than the one 5 cm above, although at reduced levels. The region 1 cm above the top edge of the LES is less exposed to acidity than the intrasphincteric zone, demonstrating efficacy of fundoplication.

Spin-dynamics in a p(bar p) ring

International Nuclear Information System (INIS)

Pisent, A.

1990-01-01

In this paper after a short introduction on the main concepts of spin dynamics, like the conservation of the polarization as a stability condition, the depolarizing resonances and their care by the Siberian Snake schema, two particular applications are discussed. In the European hadron Facility, and in the other future hadron machines in the same range of energies (30--40 Gev), the polarization in the main ring can be maintained using a Siberian Snake. We shall discuss the design of such a device. As a second example is considered the Spin Splitter, a proposed experiment with the aim of polarizing bar p. Also in this case the spin stability is realized by the Siberian Snake schema

Physiological growth and yield evaluation in p-mineralized sunflower (helianthus annus l.) under sudano-sahelian agro-ecology, nigeria

International Nuclear Information System (INIS)

Wabekwa, J.W.; Sodagni, I.A.; Mohammad, F.K

2013-01-01

The study was carried out at the Research and Demonstration Farm, Department of Crop Production, University of Maiduguri (11 degree 47 'N, 13 degree 13 'E) during 2010 and 2011 rainy seasons, to investigate the response of sunflower to phosphorus fertilizer inorganically applied as P/sub 2/O/sub 5/ at the rate 0, 20, 40, 60 and 80 kg ha . The experimental design was randomized complete block and replicated thrice. It was observed that leaf area per plant and plant dry weight increased with increasing P/sub 2/ O/sub 5/ rates (40-60 kg ha ) at 6, 8 and 10 weeks after sowing (WAS) . Crop growth rates similarly increased with increasing P/sub 2/O/sub 5/ rates (40-80 kg ha/sup -1/) at 6 WAS in both years and the combined means for the two years, and at 8 WAS in 2011 and the combined analysis. Days to 50% flowering and head dry weight were not significantly influenced by P/sub 2/O/sub 5/ rates and 1000- grain weights indicated that increased P/sub 2/O/sub 5/P/sub 2/O/sub 5/ application (40-80 kg ha ) enhanced grain filling efficiency and this influenced weight per 1000 - grains during both years. The application of 40 kg P O ha increased grain yield statistically in 2010 rainy season (2289.10 kg ha ) and the combined analysis (1565.20 kg ha). During 2011 rainy season, 80 kg P/sub 2/ O/sub 5/ ha/sup -1/recorded statistically higher grain yield (1153.60 kg ha). Similarly, the application of 80 kg P O ha statistically influenced higher fodder yield (4112.60 kg ha ) in 2010 rainy season; while 60 kg P/sub 2/O/sub 5/ kg ha yielded statistically higher fodder values during 2011 (1873.00 kg ha/sup 1/ ) and combined analysis (2393.30 kg ha ). Application of 60 kg P/sub 2/O/sub 5/ ha also increased the biological yield statistically in 2010 (3709.70 kg ha/sup -1) and lower P/sub 2/ O/sub 5/ rate of 40 kg ha statistically yielded 3980.80 kg ha in the combined analysis while biological yield was not statistically significant during 2011 rainy season. Considering 40 kg P/sub 2/O

40 CFR 721.3720 - Fatty amide.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Fatty amide. 721.3720 Section 721.3720... Fatty amide. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified generically as a fatty amide (PMN P-91-87) is subject to reporting under this section...

40 CFR 721.2120 - Cyclic amide.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Cyclic amide. 721.2120 Section 721... Cyclic amide. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified as a cyclic amide (PMN P-92-131) is subject to reporting under this section for the...

Mid-IR optical properties of silicon doped InP

DEFF Research Database (Denmark)

Panah, Mohammad Esmail Aryaee; Han, Li; Norrman, Kion

2017-01-01

of growth conditions on the optical and electrical properties of silicon doped InP (InP:Si) in the wavelength range from 3 to 40 μm was studied. The carrier concentration of up to 3.9 × 1019 cm-3 is achieved by optimizing the growth conditions. The dielectric function, effective mass of electrons and plasma...

40 CFR 40.135-1 - Preapplication coordination.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Preapplication coordination. 40.135-1 Section 40.135-1 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE RESEARCH AND DEMONSTRATION GRANTS § 40.135-1 Preapplication coordination. (a) All applicants. (1...

Concurrent disruption of p16INK4a and the ARF-p53 pathway predicts poor prognosis in aggressive non-Hodgkin's lymphoma

DEFF Research Database (Denmark)

Grønbaek, K; de Nully Brown, P; Møller, Michael Boe

2000-01-01

. By using a panel of PCR-based methods, we have examined the status of the p16INK4a, ARF and p53 genes in 123 cases of non-Hodgkin's lymphoma (NHL) at diagnosis. Alterations of one or more of these genes were detected in seven of 36 (19%) cases with low- to intermediate-grade histology, and in 35 of 87 (40...

40 CFR 721.9973 - Zirconium dichlorides (generic).

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Zirconium dichlorides (generic). 721... Substances § 721.9973 Zirconium dichlorides (generic). (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substances identified generically as zirconium dichlorides (PMNs P...

All-Optical Regenerative OTDM Add/Drop Multiplexing at 40 Gbit/s using Monolithic InP Mach-Zehnder Interferometer

DEFF Research Database (Denmark)

Buxens, Alvaro A.; Clausen, Anders; Poulsen, Henrik Nørskov

2000-01-01

We report a novel method for simultaneous add-drop multiplexing in OTDM systems which includes regenerative capabilities and perfect clearing of the drop channel time slot. The principle has been demonstrated at 40 Gb/s showing an excellent performance.......We report a novel method for simultaneous add-drop multiplexing in OTDM systems which includes regenerative capabilities and perfect clearing of the drop channel time slot. The principle has been demonstrated at 40 Gb/s showing an excellent performance....

Mesothelioma Cells Escape Heat Stress by Upregulating Hsp40/Hsp70 Expression via Mitogen-Activated Protein Kinases

Directory of Open Access Journals (Sweden)

Michael Roth

2009-01-01

Full Text Available Therapy with hyperthermal chemotherapy in pleural diffuse malignant mesothelioma had limited benefits for patients. Here we investigated the effect of heat stress on heat shock proteins (HSP, which rescue tumour cells from apoptosis. In human mesothelioma and mesothelial cells heat stress (39–42°C induced the phosphorylation of two mitogen activated kinases (MAPK Erk1/2 and p38, and increased Hsp40, and Hsp70 expression. Mesothelioma cells expressed more Hsp40 and were less sensitive to heat stress compared to mesothelial cells. Inhibition of Erk1/2 MAPK by PD98059 or by Erk1 siRNA down-regulated heat stress-induced Hsp40 and Hsp70 expression and reduced mesothelioma cell survival. Inhibition of p38MAPK by SB203580 or siRNA reduced Hsp40, but not Hsp70, expression and also increased mesothelioma cell death. Thus hyperthermia combined with suppression of p38 MAPK or Hsp40 may represent a novel approach to improve mesothelioma therapy.

Increased concentrations of soluble CD40 ligand platelet in patients with primary antiphospholipidic syndrome.

Science.gov (United States)

Galicia López, Aida; Olguín Ortega, Lourdes; Saavedra, Miguel A; Méndez Cruz, René; Jimenez Flores, Rafael; García de la Peña, Maximiliano

2013-01-01

To determine the concentrations of sCD40L in patients with PAPS, and establish its association with the number of thrombosis. We included patients with PAPS and healthy controls of the same age and sex. For analysis, patients with PAPS were divided into 2 groups: 1) patients with 1 thrombosis, and 2) patients with >1 thrombosis. Soluble CD40L concentrations were determined by ELISA method. sCD40L concentrations were significantly higher in patients with PAPS compared with the controls (9.72 ng ± 11.23 ng/ml vs. 4.69 ± 4.04 ng/ml) (P=.04) There was no association between serum levels of sCD40L and the number of thrombosis (1 thrombosis: 9.81 ± 9.87 ng/ml vs 9.63 ± 12.75 ng/ml in ≥ 1thrombosis (P=.13). In women with pregnancy and abortions, (13 patients) concentrations of sCD40L were higher than in those patients without a history of abortion (26 patients) but without statically significant difference (12.11 ± 16.46 ng/ml vs. 8.80 ± 8.61 ng/ml) (P=.33). There was no correlation between levels of sCD40L and the total number of thrombosis. Patients with PAPS have higher concentrations of sCD40L compared with healthy subjects, although this is not associated with a greater number of thrombosis. Among patients with PAPS, there is a tendency to higher concentrations of sCD40L in women with pregnancy and history of abortion. Since the platelet is the main cellular source of sCD40L, is possible that this pathway plays a pathogenic role in patients with PAPS. Copyright © 2012 Elsevier España, S.L. All rights reserved.

Two-particle correlations in azimuthal angle and pseudorapidity in inelastic p + p interactions at the CERN Super Proton Synchrotron

International Nuclear Information System (INIS)

Aduszkiewicz, A.; Dominik, W.; Kuich, M.; Matulewicz, T.; Posiadala, M.; Walewski, M.; Ali, Y.; Andronov, E.; Feofilov, G.A.; Igolkin, S.; Kondratiev, V.P.; Kovalenko, V.; Merzlaya, A.; Seryakov, A.; Vechernin, V.V.; Vinogradov, L.; Anticic, T.; Kadija, K.; Susa, T.; Antoniou, N.; Christakoglou, P.; Diakonos, F.; Kapoyannis, A.; Panagiotou, A.D.; Vassiliou, M.; Baatar, B.; Brandin, A.; Selyuzhenkov, I.; Taranenko, A.; Bay, F.; Di Luise, S.; Rubbia, A.; Sgalaberna, D.; Blondel, A.; Bravar, A.; Damyanova, A.; Haesler, A.; Korzenev, A.; Ravonel, M.; Bogomilov, M.; Kolev, D.; Tsenov, R.; Brzychczyk, J.; Larsen, D.; Planeta, R.; Richter-Was, E.; Staszel, P.; Wyszynski, O.; Bunyatov, S.A.; Kolesnikov, V.I.; Krasnoperov, A.; Lyubushkin, V.V.; Malakhov, A.I.; Matveev, V.; Melkumov, G.L.; Tereshchenko, V.; Busygina, O.; Golubeva, M.; Guber, F.; Ivashkin, A.; Kurepin, A.; Sadovsky, A.; Cirkovic, M.; Knezevic, N.; Manic, D.; Puzovic, J.; Czopowicz, T.; Grebieszkow, K.; Mackowiak-Pawlowska, M.; Maksiak, B.; Sarnecki, R.; Slodkowski, M.; Tefelska, A.; Tefelski, D.; Davis, N.; Kielbowicz, M.; Ozvenchuk, V.; Rybicki, A.; Dembinski, H.; Engel, R.; Herve, A.E.; Mathes, H.J.; Roth, M.; Szuba, M.; Ulrich, R.; Unger, M.; Veberic, D.; Deveaux, M.; Koziel, M.; Renfordt, R.; Stroebele, H.; Dumarchez, J.; Robert, A.; Ereditato, A.; Hierholzer, M.; Nirkko, M.; Pistillo, C.; Redij, A.; Fodor, Z.; Garibov, A.; Gazdzicki, M.; Grzeszczuk, A.; Kaptur, E.; Kisiel, J.; Kowalski, S.; Pulawski, S.; Schmidt, K.; Wilczek, A.; Hasegawa, T.; Kobayashi, T.; Nakadaira, T.; Nishikawa, K.; Sakashita, K.; Sekiguchi, T.; Shibata, M.; Tada, M.; Hylen, J.; Lundberg, B.; Marchionni, A.; Rameika, R.; Zwaska, R.; Johnson, S.R.; Marino, A.D.; Nagai, Y.; Rumberger, B.T.; Zimmerman, E.D.; Kowalik, K.; Rondio, E.; Stepaniak, J.; Laszlo, A.; Marton, K.; Vesztergombi, G.; Lewicki, M.; Naskret, M.; Turko, L.; Marcinek, A.; Messerly, B.; Paolone, V.; Wickremasinghe, A.; Mills, G.B.; Morozov, S.; Petukhov, O.; Mrowczynski, S.; Rybczynski, M.; Seyboth, P.; Stefanek, G.; Wlodarczyk, Z.; Wojtaszek-Szwarc, A.; Pavin, M.; Popov, B.A.; Rauch, W.; Roehrich, D.; Rustamov, A.; Zambelli, L.

2017-01-01

Results on two-particle ΔηΔφ correlations in inelastic p + p interactions at 20, 31, 40, 80, and 158 GeV/c are presented. The measurements were performed using the large acceptance NA61/SHINE hadron spectrometer at the CERN Super Proton Synchrotron. The data show structures which can be attributed mainly to effects of resonance decays, momentum conservation, and quantum statistics. The results are compared with the Epos and UrQMD models. (orig.)

Two-particle correlations in azimuthal angle and pseudorapidity in inelastic p + p interactions at the CERN Super Proton Synchrotron

Energy Technology Data Exchange (ETDEWEB)

Aduszkiewicz, A.; Dominik, W.; Kuich, M.; Matulewicz, T.; Posiadala, M.; Walewski, M. [University of Warsaw, Warsaw (Poland); Ali, Y. [Jagiellonian University, Cracow (Poland); COMSATS Institute of Information Technology, Islamabad (Pakistan); Andronov, E.; Feofilov, G.A.; Igolkin, S.; Kondratiev, V.P.; Kovalenko, V.; Merzlaya, A.; Seryakov, A.; Vechernin, V.V.; Vinogradov, L. [St. Petersburg State University, St. Petersburg (Russian Federation); Anticic, T.; Kadija, K.; Susa, T. [Ruder Boskovic Institute, Zagreb (Croatia); Antoniou, N.; Christakoglou, P.; Diakonos, F.; Kapoyannis, A.; Panagiotou, A.D.; Vassiliou, M. [University of Athens, Athens (Greece); Baatar, B.; Brandin, A.; Selyuzhenkov, I.; Taranenko, A. [National Research Nuclear University (Moscow Engineering Physics Institute), Moscow (Russian Federation); Bay, F.; Di Luise, S.; Rubbia, A.; Sgalaberna, D. [ETH Zuerich, Zuerich (Switzerland); Blondel, A.; Bravar, A.; Damyanova, A.; Haesler, A.; Korzenev, A.; Ravonel, M. [University of Geneva, Geneva (Switzerland); Bogomilov, M.; Kolev, D.; Tsenov, R. [University of Sofia, Faculty of Physics, Sofia (Bulgaria); Brzychczyk, J.; Larsen, D.; Planeta, R.; Richter-Was, E.; Staszel, P.; Wyszynski, O. [Jagiellonian University, Cracow (Poland); Bunyatov, S.A.; Kolesnikov, V.I.; Krasnoperov, A.; Lyubushkin, V.V.; Malakhov, A.I.; Matveev, V.; Melkumov, G.L.; Tereshchenko, V. [Joint Institute for Nuclear Research, Dubna (Russian Federation); Busygina, O.; Golubeva, M.; Guber, F.; Ivashkin, A.; Kurepin, A.; Sadovsky, A. [Institute for Nuclear Research, Moscow (Russian Federation); Cirkovic, M.; Knezevic, N.; Manic, D.; Puzovic, J. [University of Belgrade, Belgrade (Serbia); Czopowicz, T.; Grebieszkow, K.; Mackowiak-Pawlowska, M.; Maksiak, B.; Sarnecki, R.; Slodkowski, M.; Tefelska, A.; Tefelski, D. [Warsaw University of Technology, Warsaw (Poland); Davis, N.; Kielbowicz, M.; Ozvenchuk, V.; Rybicki, A. [H. Niewodniczanski Institute of Nuclear Physics of the Polish Academy of Sciences, Krakow (Poland); Dembinski, H.; Engel, R.; Herve, A.E.; Mathes, H.J.; Roth, M.; Szuba, M.; Ulrich, R.; Unger, M.; Veberic, D. [Karlsruhe Institute of Technology, Karlsruhe (Germany); Deveaux, M.; Koziel, M.; Renfordt, R.; Stroebele, H. [University of Frankfurt, Frankfurt (Germany); Dumarchez, J.; Robert, A. [LPNHE, University of Paris VI and VII, Paris (France); Ereditato, A.; Hierholzer, M.; Nirkko, M.; Pistillo, C.; Redij, A. [University of Bern, Bern (Switzerland); Fodor, Z. [Wigner Research Centre for Physics of the Hungarian Academy of Sciences, Budapest (Hungary); University of Wroclaw, Wroclaw (Poland); Garibov, A. [National Nuclear Research Center, Baku (Azerbaijan); Gazdzicki, M. [University of Frankfurt, Frankfurt (Germany); Jan Kochanowski University in Kielce, Kielce (Poland); Grzeszczuk, A.; Kaptur, E.; Kisiel, J.; Kowalski, S.; Pulawski, S.; Schmidt, K.; Wilczek, A. [University of Silesia, Katowice (Poland); Hasegawa, T.; Kobayashi, T.; Nakadaira, T.; Nishikawa, K.; Sakashita, K.; Sekiguchi, T.; Shibata, M.; Tada, M. [Institute for Particle and Nuclear Studies, Tsukuba (Japan); Hylen, J.; Lundberg, B.; Marchionni, A.; Rameika, R.; Zwaska, R. [Fermilab, Batavia (United States); Johnson, S.R.; Marino, A.D.; Nagai, Y.; Rumberger, B.T.; Zimmerman, E.D. [University of Colorado, Boulder (United States); Kowalik, K.; Rondio, E.; Stepaniak, J. [National Centre for Nuclear Research, Warsaw (Poland); Laszlo, A.; Marton, K.; Vesztergombi, G. [Wigner Research Centre for Physics of the Hungarian Academy of Sciences, Budapest (Hungary); Lewicki, M.; Naskret, M.; Turko, L. [University of Wroclaw, Wroclaw (Poland); Marcinek, A. [Jagiellonian University, Cracow (Poland); University of Wroclaw, Wroclaw (Poland); Messerly, B.; Paolone, V.; Wickremasinghe, A. [University of Pittsburgh, Pittsburgh (United States); Mills, G.B. [Los Alamos National Laboratory, Los Alamos (United States); Morozov, S.; Petukhov, O. [Institute for Nuclear Research, Moscow (Russian Federation); National Research Nuclear University (Moscow Engineering Physics Institute), Moscow (Russian Federation); Mrowczynski, S.; Rybczynski, M.; Seyboth, P.; Stefanek, G.; Wlodarczyk, Z.; Wojtaszek-Szwarc, A. [Jan Kochanowski University in Kielce, Kielce (Poland); Pavin, M. [Ruder Boskovic Institute, Zagreb (Croatia); LPNHE, University of Paris VI and VII, Paris (France); Popov, B.A. [LPNHE, University of Paris VI and VII, Paris (France); Joint Institute for Nuclear Research, Dubna (Russian Federation); Rauch, W. [Fachhochschule Frankfurt, Frankfurt (Germany); Roehrich, D. [University of Bergen, Bergen (Norway); Rustamov, A. [National Nuclear Research Center, Baku (Azerbaijan); University of Frankfurt, Frankfurt (Germany); Zambelli, L. [LPNHE, University of Paris VI and VII, Paris (France); Institute for Particle and Nuclear Studies, Tsukuba (Japan)

2017-02-15

Results on two-particle ΔηΔφ correlations in inelastic p + p interactions at 20, 31, 40, 80, and 158 GeV/c are presented. The measurements were performed using the large acceptance NA61/SHINE hadron spectrometer at the CERN Super Proton Synchrotron. The data show structures which can be attributed mainly to effects of resonance decays, momentum conservation, and quantum statistics. The results are compared with the Epos and UrQMD models. (orig.)

The pH of beverages in the United States.

Science.gov (United States)

Reddy, Avanija; Norris, Don F; Momeni, Stephanie S; Waldo, Belinda; Ruby, John D

2016-04-01

Dental erosion is the chemical dissolution of tooth structure in the absence of bacteria when the environment is acidic (pH beverage's erosive potential. In addition, citrate chelation of calcium ions may contribute to erosion at higher pH. The authors of this study determined the erosive potential measured by the pH of commercially available beverages in the United States. The authors purchased 379 beverages from stores in Birmingham, Alabama, and categorized them (for example, juices, sodas, flavored waters, teas, and energy drinks) and assessed their pH. They used a pH meter to measure the pH of each beverage in triplicate immediately after it was opened at a temperature of 25°C. The authors recorded the pH data as mean (standard deviation). Most (93%, 354 of 379) beverages had a pH of less than 4.0, and 7% (25 of 379) had a pH of 4.0 or more. Relative beverage erosivity zones based on studies of apatite solubility in acid indicated that 39% (149 of 379) of the beverages tested in this study were considered extremely erosive (pH beverages in the United States found that most are potentially erosive to the dentition. This study's findings provide dental clinicians and auxiliaries with information regarding the erosive potential of commercially available beverages. Specific dietary recommendations for the prevention of dental erosion may now be developed based on the patient's history of beverage consumption. Copyright © 2016 American Dental Association. Published by Elsevier Inc. All rights reserved.

Generation of particles with large transverse momenta in π--p- and π-12C-interactions at 40 GeV/c

International Nuclear Information System (INIS)

Jordanova, Yu.; Lyubimov, V.; Mitova, S.; Penev, V.N.; Shklovskaya, A.

1981-01-01

The generation of a particle with transverse momenta Psub(transverse)>0.8 GeV/c in π - -p- and π - - 12 C-interactions with a π - -meson beam at a momentum Psub(π)=40 GeV/c in a two-meter propan chamber is investigated. Analyses of the secondary particle correlations produced in the interactions, in which the emitted hadron or group of hadrons have large transverse momenta, are carried out. In the investigated interactions the secondary particle momenta are measured with an accuracy not less than 30%. In the experimental data treatment of the π - - 12 C-collisions the interactions of π - -mesons with the quasifree nucleons of 12 C-nucleus are taken into account on the basis of the multiperipherical model. The experimental data analyses indicate that: 1) In the events with one or more secondary particles having large momenta Psub(transverse)>0.8 GeV/c the asymmetric correlations increase for both π - -p- and π - - 12 C-interactions; 2) Most correlated pairs of secondary particles having Psub(transverse)>0.8 GeV/c are produced with an almost equal rapidity and transverse momenta; 3) The presence of the secondary particles with large transverse momenta does not influence the resonance formation (in particular rho 0 -meson); 4) The effective mass distribution of the two secondary particles with Psub(transverse)>0.8 GeV/c has a broad peak at about 2 GeV/c

Characterization of components released by alkali disruption of simian virus 40

DEFF Research Database (Denmark)

Christiansen, Gunna; Landers, T; Griffith, J

1977-01-01

Treatment of simian virus 40 (SV40) particles at pH 9.8 in the presence of 1 mM dithiothreitol for 5 min at 37 degrees C disrupted the virions into a 60S DNA-protein complex and DNA-free 7S protein particles. The DNA-protein complex contained approximately equal amounts of DNA and protein, and ap...

Two-particle correlations in p+p and Pb+Pb collisions at SPS energies

CERN Document Server

AUTHOR|(CDS)2081214

In this thesis two-particle correlations in pseudorapidity and azimuthal angle in p+p collisions at beam momenta: 20, 31, 40, 80, and 158 GeV/c are presented. Data were recorded in the NA61/SHINE experiment at the CERN Super Proton Synchrotron (SPS). The results are compared to the EPOS and the UrQMD models as well as to the results from various experiments at the Relativistic Heavy-Ion Collider (RHIC) and the Large Hadron Collider (LHC). Dedicated comparison analysis was done also on NA49 data of Pb+Pb collisions. The inclusive results in p+p show correlation structures connected with resonance decays, Bose-Einstein statistics, momentum conservation, and strings fragmentation. No structures connected with hard processes were observed even at 158 GeV/c beam momentum. The EPOS model reproduces data fine except of Bose-Einstein enhancement; the UrQMD model shows many disagreements with data. The results provide an insight into forgotten realm of soft physics where jet peaks do not cast shadows onto two-particle...

40 CFR 721.9959 - Polyurethane polymer (generic).

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Polyurethane polymer (generic). 721... Substances § 721.9959 Polyurethane polymer (generic). (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified generically as a polyurethane polymer (PMN P-01...

A simple enzymic method for the synthesis of [32P]phosphoenolpyruvate

International Nuclear Information System (INIS)

Parra, F.

1982-01-01

A rapid and simple enzymic method is described for the synthesis of [ 32 P]phosphoenolpyruvate from [ 32 P]Psub(i), with a reproducible yield of 74%. The final product was shown to be a good substrate for pyruvate kinase (EC 2.7.1.40). (author)

Intraband light absorption by holes in InGaAsP/InP quantum wells

Science.gov (United States)

Pavlov, N. V.; Zegrya, G. G.

2018-03-01

A microscopic analysis of the mechanism of intraband radiation absorption by holes with their transition to a spin-split band for quantum wells based on InGaAsP/InP solid solutions is performed within the framework of the four-band Kane model. The calculation is made for two polarizations of the incident radiation: along the crystal growth axis and in the plane of the quantum well. It is shown that this process can be the main mechanism of internal radiation losses for quantum well lasers. It is also shown that the dependence of the absorption coefficient on the width of the quantum well has a maximum at a well width from 40 to 60 A.

Acidic pH facilitates peripheral αβmeATP-mediated nociception in rats: differential roles of P2X, P2Y, ASIC and TRPV1 receptors in ATP-induced mechanical allodynia and thermal hyperalgesia.

Science.gov (United States)

Seo, Hyoung-Sig; Roh, Dae-Hyun; Kwon, Soon-Gu; Yoon, Seo-Yeon; Kang, Suk-Yun; Moon, Ji-Young; Choi, Sheu-Ran; Beitz, Alvin J; Lee, Jang-Hern

2011-03-01

Peripheral ischemia is commonly associated with an increase in tissue ATP concentration and a decrease in tissue pH. Although in vitro data suggest that low tissue pH can affect ATP-binding affinities to P2 receptors, the mechanistic relationship between ATP and low pH on peripheral nociception has not been fully examined. This study was designed to investigate the potential role of an acidified environment on intraplantar αβmeATP-induced peripheral pain responses in rats. The mechanical allodynia (MA) produced by injection of αβmeATP was significantly increased in animals that received the drug diluted in pH 4.0 saline compared to those that received the drug diluted in pH 7.0 saline. Moreover, animals injected with αβmeATP (100 nmol) in pH 4.0 saline developed thermal hyperalgesia (TH), which did not occur in animals treated with αβmeATP diluted in pH 7.0 saline. To elucidate which receptors were involved in this pH-related facilitation of αβmeATP-induced MA and TH, rats were pretreated with PPADS (P2 antagonist), TNP-ATP (P2X antagonist), MRS2179 (P2Y1 antagonist), AMG9810 (TRPV1 antagonist) or amiloride (ASIC blocker). Both PPADS and TNP-ATP dose-dependently blocked pH-facilitated MA, while TH was significantly reduced by pre-treatment with MRS2179 or AMG9810. Moreover, amiloride injection significantly reduced low pH-induced facilitation of αβmeATP-mediated MA, but not TH. These results demonstrate that low tissue pH facilitates ATP-mediated MA via the activation of P2X receptors and ASICs, whereas TH induced by ATP under low pH conditions is mediated by the P2Y1 receptor and TRPV1, but not ASIC. Thus distinct mechanisms are responsible for the development of MA and TH under conditions of tissue acidosis and increased ATP. Copyright © 2010 Elsevier Ltd. All rights reserved.

Bibliography on Cold Regions Science and Technology. Volume 40, Part 2, 1986

Science.gov (United States)

1986-12-01

petroleum wells. Tsylor, A., et al, [1985, p.95-99, eng] 40-130« Winter temperaturea of a palsa bog in Finnish Lapland Sepplll, M., (1983, p.20-24. fta ...IMl, p.l4-9S, na] 40401 Tmnralun fMd of rockt in Ibc upper VUyuy River viiUy Bclkkov. L.P., et •!, [ 1911, p.9S-101, nii] 40403 Swemp IOU

Overexpression of chitinase like protein YKL-40 in leukemia patients

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Anil K. Hurmale

2013-01-01

Full Text Available YKL-40 is a member of mammalian chitanase (CHI3L1, expressed and secreted by several types of solid tumor cells, inflammatory cells and stem cells. The precise physiological role of YKL-40 in cancer is still not clear and it is suggested that it play a role in cancer cell proliferation, differentiation, metastatic potential, cell attachment and migration, reorganization and tissue remodeling.The aim of the study was to check the appearance of YKL-40 in leukemic cells and over-expression of YKL-40 in the plasma of leukemia patients in comparison to healthy controls, and find whether YKL-40 could serve as a peripheral biomarker for leukemia. The study was conducted between July 2012 and March 2013 and included 67 volunteers, 55 having leukemia at the stage of diagnosis ofthe disease and 12 normal healthy volunteers. YKL-40 levels were determined in all plasma samples using the YKL-40 enzyme-linked immunosorbent assay (ELISA kit and expression of YKL-40 was observed by using immunocytochemical (ICC analysis. YKL-40 plasma levels differed significantly between patients with leukemia and the normal healthy volunteers (P=<0.001 and YKL-40 was positively expressed in all four types of leukemia (AML, ALL, CLL and CML specimens.

YKL-40 tissue expression and plasma levels in patients with ovarian cancer

DEFF Research Database (Denmark)

Høgdall, Estrid V S; Ringsholt, Merete; Høgdall, Claus K

2009-01-01

survival. The aim of the study was to determine the expression of YKL-40 in tumor tissue and plasma in patients with borderline ovarian tumor or epithelial ovarian cancer (OC), and investigate prognostic value of this marker. METHODS: YKL-40 protein expression was determined by immunohistochemistry...... in tissue arrays from 181 borderline tumors and 473 OC. Plasma YKL-40 was determined by ELISA in preoperative samples from 19 patients with borderline tumor and 76 OC patients. RESULTS: YKL-40 protein expression was found in cancer cells, tumor associated macrophages, neutrophils and mast cells. The tumor...... stage, age and radicality after primary surgery as variables, showed that elevated plasma YKL-40 was associated with a shorter survival (HR = 2.13, 95% CI: 1.40-3.25, p = 0.0004). CONCLUSION: YKL-40 in OC tissue and plasma are related to stage and histology, but only plasma YKL-40 is a prognostic...

40 CFR 750.40 - Cross-examination.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Cross-examination. 750.40 Section 750... Processing and Distribution in Commerce Exemptions § 750.40 Cross-examination. (a) After the close of the... cross-examination. The request must be received by EPA within one week after a full transcript of the...

40 CFR 721.9635 - Terpene residue distillates.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Terpene residue distillates. 721.9635... Substances § 721.9635 Terpene residue distillates. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified generically as terpene residue distillates (PMN P-96-897...

Investigation of physicochemical factors affecting the stability of a pH-modulated solid dispersion and a tablet during storage.

Science.gov (United States)

Tran, Phuong Ha-Lien; Tran, Thao Truong-Dinh; Park, Jun-Bom; Min, Dong Hun; Choi, Han-Gon; Han, Hyo-Kyung; Rhee, Yun-Seok; Lee, Beom-Jin

2011-07-29

The stability of solid dispersions (SD) during storage is of concern. We prepared the pH-modulated SD (pSD) and compressed tablets consisting of polyethylene glycol (PEG) 6000 as a carrier, drug and MgO (alkalizer). Telmisartan (TEL), an ionizable poorly water-soluble drug, was chosen as a model drug. The changes in physicochemical factors such as the dissolution rate, drug crystallinity, microenvironmental pH (pH(M)) and intermolecular interactions of the pSD and the tablets were investigated over 3 months under different temperature and relative humidity (RH) conditions: refrigerator (5-8 °C), 25 °C/32% RH, 25 °C/55% RH, 25 °C/75% RH, 40°C/32% RH, 40 °C/55% RH, and 40 °C/75% RH. Differential scanning calorimetry (DSC) analysis of all samples revealed no distinct changes in the drug melting point. In contrast, powder X-ray diffraction (PXRD) diffractograms revealed that samples stored at 40 °C/75% RH for 1 month, 25 °C/75% RH for 3 months and 40 °C at all humidity conditions for 3 months showed gradual recrystallization of the drug. Fourier transform infrared (FTIR) spectra indicated a reduced intensity of intermolecular interactions between TEL and MgO in the pSD and tablet. The pH(M) also gradually decreased. These altered physicochemical factors under the stressed conditions resulted in decreased dissolution profiles in intestinal fluid (pH 6.8). In contrast, the dissolution rate in gastric fluid (pH 1.2) was almost unchanged because of the high intrinsic solubility of TEL at this pH. Copyright © 2011 Elsevier B.V. All rights reserved.

Tampão peridural com dextran 40 na profilaxia da cefaléia pós-punção acidental da duramáter em paciente HIV positivo: relato de caso Tampón peridural con dextran 40 en la profilaxia de la cefalea pós-punción accidental de la duramáter en paciente SIDA positivo: relato de caso Epidural patch with dextran 40 to prevent postdural puncture headache in an HIV patient: case report

Directory of Open Access Journals (Sweden)

Marcos Guilherme Cunha Cruvinel

2002-11-01

Full Text Available JUSTIFICATIVA E OBJETIVOS: A cefaléia pós-punção de duramáter é uma complicação bem conhecida das anestesias subaracnóideas e peridurais, sendo o tampão sangüíneo considerado o tratamento mais eficaz, até o momento. Este é um procedimento invasivo e sujeito a complicações graves. Seu uso em certos pacientes, como portadores de HIV ou leucemias, é motivo de debate. Várias alternativas têm sido relatadas. O objetivo deste artigo é apresentar um caso do uso do tampão peridural com dextran 40 na profilaxia da cefaléia pós-punção de duramáter em paciente portador do vírus da imunodeficiência humana (HIV, com história de cefaléia em anestesia subaracnóidea anterior. RELATO DE CASO: Paciente masculino, 31 anos, 70 kg, estado físico ASA II, portador de HIV, para tratamento de condilomatose anal recidivada, com relato de cefaléia intensa e limitante durante duas semanas após anestesia subaracnóidea (agulha Quincke 25G. Durante tentativa de anestesia peridural com agulha de Tuohy 18G em L3-L4, houve perfuração acidental da duramáter. Foram injetados, por duas vezes, 20 ml de dextran 40 a 10% por cateter peridural; a primeira, 150 minutos após a administração dos anestésicos e a segunda na manhã seguinte à cirurgia. O paciente evoluiu assintomático e recebeu alta no dia seguinte à sua internação. CONCLUSÕES: O uso do tampão com soluções colóides como o dextran 40 não está bem estabelecido, porém existem alguns relatos do seu uso com sucesso e entendemos que seu potencial deva ser melhor explorado.JUSTIFICATIVA Y OBJETIVOS: La cefalea pós-punción de duramáter es una complicación bien conocida de las anestesias subaracnóideas y peridurales, siendo el tampón sanguíneo considerado el tratamiento más eficaz hasta el momento. Este es un procedimiento invasivo y sujeto a complicaciones graves. Su uso en ciertos pacientes, como portadores de SIDA o leucemias, es motivo de debate. Varias alternativas

Hubungan Faktor Risiko, Status Instabilitas Mikrosatelit, dan Ekspresi P53 dengan Karsinogenesis Adenokarsinoma Kolorektal pada Orang Indonesia

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Kiki Lukman

2012-12-01

Full Text Available Different from developed countries, molecular characteristic of colorectal adenocarcinoma (CRC among Indonesians in the age group of ≤40 years old is mostly similar compared to the CRC of the age group of >40 years old, and both are sporadic cancers. To know the association of molecular characteristic with the risk factors of CRC, a cross sectional study was conducted to analyze the association of risk factors, microsatellite instability (MSI and P53 expression (chromosomal Instability=CIN status in 39 consecutive CRC patients who were eligible for this study at Dr Hasan Sadikin Hospital, from March 2009 until March 2010. They consisted of 21 male and 18 female patients. Of them, there were 17 and 22 patients with>40 and≤40 years of age, respectively. The immuno-histochemistry examinations for the expression of mutated MLH1, MSH2 and p53 genes were conducted to determine the CIN and MSI status. The results showed that the CRC ≤40 years of age had 4 MSI high, 1 MSI low, and 17 MSI negative, associated with 10 P53 positives and 12 p53 negatives. By contrast, in the CRC >40 years of age the MSI was low and negative in 4 and 13 cases, respectively. They were associated with 11 and 6 of p53 positive and negative status, respectively. Between the two groups, there were no significant differences with regards to the association pattern between the risk factors and their molecular characteristics (p>0.05. Conclusions, majority of CRC patients among Indonesian show a molecular classification of high CIN and low MSI, and is associated with risk factors of high fat and protein, low fiber dietary intake, overweight, smoking, and low physical activitis.

Neutral meson production in $p$-Be and $p$-Au collisions at 450 GeV beam energy

CERN Document Server

Agakichiev, G; Averbeck, R; Ballester, F; Baur, R; Brenschede, A; Díaz, J; Drees, A; Faschingbauer, U; Ferrero, J L; Fischer, P; Fraenkel, Zeev; Franke, M; Fuchs, C; Gatti, E; Glässel, P; Günzel, T F; Pérez de los Heros, C; Hess, F; Holzmann, R; Irmscher, D; Jacob, C; Kühn, W; Lenkeit, B C; Löhner, H; Marin, A; Marqués, F M; Martínez, G; Metag, V; Notheisen, M; Novotny, R; Olsen, L H; Ostendorf, R W; Panebratsev, Yu A; Pfeiffer, A; Ravinovich, I; Rehak, P; Sampietro, M; Schön, A; Schükraft, Jürgen; Schutz, Y; Shimansky, S S; Shor, A; Simon, R S; Specht, H J; Steiner, V; Tapprogge, Stefan; Tel-Zur, G; Tserruya, Itzhak; Ullrich, T S; Wilschut, H W; Wurm, J P; Yurevich, V I

1998-01-01

In a joint experiment the TAPS and CERES collaborations have studied the production of the neutral mesons $\\pi^{0}$, $\\eta$ and $\\omega$ in 450 GeV $p$-Be and $p$-Au collisions at the CERN SPS. The mesons were identified by their $\\pi^{0} \\rightarrow \\gamma\\gamma, \\eta \\rightarrow \\gamma\\gamma$, and $\\omega \\rightarrow \\pi^{0}\\gamma$ decay modes. The cross sections were measured down to very low $p_{T}$ ($\\geq$ 20 MeV/c) near mid-rapidity $(3.04.0)$. The $\\eta$ and $\\omega$ cross sections are smaller than expected from extrapolations of earlier data to low $p_{T}$. Integrated over all $p_{T}$, the ratios $\\eta/\\pi^{0}$ and $\\omega/\\pi^{0}$ are lower by about 25% compared to previously published values. The reduction may be attributed to the large number of low $p_{T}$ pions from decays of heavier mesons. In addition, we have studied the dependence of neutral meson production on the mass of the nuclear target and find it in good agreement with previous measurements of charged mesons.

Estudo experimental comparando o uso do 2-octil cianoAcrilato ao nylon 4-0 na sutura da pele

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Ruffo de Freitas-Júnior

Full Text Available OBJETIVO: Comparar o 2-octil cianoacrilato com o fio de "nylon" no fechamento da pele em ratos. MÉTODOS: Vinte e cinco ratos da linhagem Wistar, foram submetidos à incisão de 3 cm de cada lado do abdome. A síntese foi feita utilizando em um dos lados o "nylon" 4.0, pontos intradérmicos, e do outro o 2-octil cianoacrilato. Após sete dias, o fio foi removido e as incisões, analisadas quanto às complicações. Após 40 dias, o resultado da cicatriz foi avaliado. Os ratos foram sacrificados, as cicatrizes foram ressecadas, fixadas e enviadas ao patologista, sem informação sobre qual o método utilizado. RESULTADOS: Houve dois óbitos durante a anestesia e um tardio. O tempo de operação foi de 136 segundos com a cola e 176 segundos, com o "nylon" (P=0,003. Dentre as 50 operações realizadas, as complicações foram: um hematoma com cada método (P=0,80, quinze deiscências da cola contra 11 do "nylon" (P=0,20, sete cicatrizes de aspecto ruim ou razoável da cola contra quatro do "nylon" (P=0,30, três infecções na cola contra duas (P=0,40. Ao exame patológico, a mediana da largura da cicatriz foi de 1.119 micra com a cola e 1.800 com o "nylon" (P=0,40. A espessura foi de 1.795 contra 1.705 micra (P=0,40. CONCLUSÃO: O 2-octil cianoacrilato apresentou o mesmo aspecto cicatricial, a mesma resistência e as mesmas complicações que a sutura com o "nylon" 4.0, porém permitindo redução no tempo cirúrgico.

/sup 40/Ar//sup 39/Ar and K-Ar dating of altered glassy volcanic rocks: the Dabi Volcanics, P. N. G

Energy Technology Data Exchange (ETDEWEB)

Walker, D.A. (Australian National Univ., Canberra. Dept. of Geology); McDougall, I. (Australian National Univ., Canberra. Research School of Earth Sciences)

1982-11-01

K-Ar and /sup 40/Ar//sup 39/Ar ages have been determined for altered submarine tholeiitic and boninite (high-Mg andesite) lavas from the Dabi Volcanics, Cape Vogel Peninsula, Papua New Guinea. /sup 40/Ar//sup 39/Ar whole rock total fusion and plateau ages identify a Late Paleocene age for the tholeiitic lavas (58.9 +- 1.1 Ma), and also for the boninitic lavas (58.8 +- 0.8 Ma). Apparent K-Ar ages for the same samples range from 27.2 +- 0.7 to 63.9 +- 4.5 Ma, and young K-Ar ages for glassy boninites are probably due to variable radiogenic /sup 40/Ar(/sup 40/Ar*) loss. These new ages effectively reconcile previously ambiguous age data for the Dabi Volcanics, and indicate contemporaneous tholeiitic and boninitic volcanism occurring in southeast PNG during the Late Paleocene. Smectites, developed as alteration products after glass in oceanic lavas commonly do not retain /sup 39/Ar during or subsequent to irradiation, but in some cases may contain /sup 40/Ar*. The results are discussed.

Electron excitation cross sections for the 2s(2)2p(3)4S(O) -- 2s(2)2p(3)2D(O) (forbidden) and 4S(O) -- 2s2p(4) 4P (resonance) transitions in O II

Science.gov (United States)

Zuo, M.; Smith, Steven J.; Chutjian, A.; Williams, I. D.; Tayal, S. S.; Mclaughlin, Brendan M.

1995-01-01

Experimental and theoretical excitation cross sections are reported for the first forbidden transition 4S(O) -- 2S(2)2p(3) 2D(O) (lambda-lambda 3726, 3729) and the first allowed (resonance) transition 4S(O) -- 2s2p(4) 4P(lambda-833) in O II. Use is made of electron energy loss and merged-beams methods. The electron energy range covered is 3.33 (threshold) to 15 eV for the S -- D transition, and 14.9 (threshold) to 40 eV for the S -- P transition. Care was taken to assess and minimize the metastable fraction of the O II beam. An electron mirror was designed and tested to reflect inelastically backscattered electrons into the forward direction to account for the full range of polar scattering angles. Comparisons are made between present experiments and 11-state R-matrix calculations. Calculations are also presented for the 4S(O) -- 2s(2)2p(3)2P(O) (lambda-2470) transition.

The supplementation of low-P diets with microbial 6-phytase expressed in Aspergillus oryzae improves P digestibility in sows.

Science.gov (United States)

Torrallardona, D; Llauradó, L; Broz, J

2012-12-01

Two trials were conducted to evaluate a novel microbial 6-phytase expressed in Aspergillus oryzae (Ronozyme HiPhos; DSM Nutritional Products, Basel, Switzerland) in gestating and lactating sows. In the first trial, 24 sows (Duroc × Landrace; 223 kg BW) were offered, at 16 d of gestation, a low-P control diet (formulated to provide 4.0 g total P/kg; 1.5 g digestible P/kg) supplemented with 0, 500, or 1000 phytase activity (FYT)/kg of phytase. Two weeks later, fresh feces were sampled from all sows and the apparent total tract digestibility of P was measured using TiO(2) as indigestible marker. Phytase supplementation did not (P > 0.10) affect the total tract digestibility of P but reduced (P phytase. After 2 wk, fresh feces were sampled from all sows and the apparent total tract digestibility of P was measured using TiO(2) as indigestible marker. Phytase supplementation improved (P phytase tested increased the apparent total tract digestibility of P in sows and reduced P excretion in feces.

Biodistribution of the GATA-3-specific DNAzyme hgd40 after inhalative exposure in mice, rats and dogs

International Nuclear Information System (INIS)

Turowska, Agnieszka; Librizzi, Damiano; Baumgartl, Nadja; Kuhlmann, Jens; Dicke, Tanja; Merkel, Olivia; Homburg, Ursula; Höffken, Helmut; Renz, Harald; Garn, Holger

2013-01-01

The DNAzyme hgd40 was shown to effectively reduce expression of the transcription factor GATA-3 RNA which plays an important role in the regulation of Th2-mediated immune mechanisms such as in allergic bronchial asthma. However, uptake, biodistribution and pharmacokinetics of hgd40 have not been investigated yet. We examined local and systemic distribution of hgd40 in naive mice and mice suffering from experimental asthma. Furthermore, we evaluated the pharmacokinetics as a function of dose following single and repeated administration in rats and dogs. Using intranasal administration of fluorescently labeled hgd40 we demonstrated that the DNAzyme was evenly distributed in inflamed asthmatic mouse lungs within minutes after single dose application. Systemic distribution was investigated in mice using radioactive labeled hgd40. After intratracheal application, highest amounts of hgd40 were detected in the lungs. High amounts were also detected in the bladder indicating urinary excretion as a major elimination pathway. In serum, low systemic hgd40 levels were detected already at 5 min post application (p.a.), subsequently decreasing over time to non-detectable levels at 2 h p.a. As revealed by Single Photon Emission Computed Tomography, trace amounts of hgd40 were detectable in lungs up to 7 days p.a. Also in the toxicologically relevant rats and dogs, hgd40 was detectable in blood only shortly after inhalative application. The plasma pharmacokinetic profile was dose and time dependent. Repeated administration did not lead to drug accumulation in plasma of dogs and rats. These pharmacokinetic of hgd40 provide guidance for clinical development, and support an infrequent and convenient dose administration regimen. - Highlights: • Local and systemic distribution of GATA-3-specific DNAzyme hgd40 was investigated. • Pharmacokinetics of hgd40 was tested in rats and dogs. • hgd40 dissolved in PBS was easily taken up into the lungs after local application. • No

Biodistribution of the GATA-3-specific DNAzyme hgd40 after inhalative exposure in mice, rats and dogs

Energy Technology Data Exchange (ETDEWEB)

Turowska, Agnieszka [sterna biologicals GmbH and Co. KG, Marburg (Germany); Librizzi, Damiano [Department of Nuclear Medicine, University Hospital Giessen and Marburg GmbH, Baldingerstrasse, 35043 Marburg (Germany); Baumgartl, Nadja [Institute of Laboratory Medicine and Pathobiochemistry-Molecular Diagnostics, Philipps University of Marburg (Germany); Kuhlmann, Jens; Dicke, Tanja [sterna biologicals GmbH and Co. KG, Marburg (Germany); Merkel, Olivia [Department of Pharmaceutical Sciences, Wayne State University, Detroit (United States); Homburg, Ursula [sterna biologicals GmbH and Co. KG, Marburg (Germany); Höffken, Helmut [Department of Nuclear Medicine, University Hospital Giessen and Marburg GmbH, Baldingerstrasse, 35043 Marburg (Germany); Renz, Harald [Institute of Laboratory Medicine and Pathobiochemistry-Molecular Diagnostics, Philipps University of Marburg (Germany); Garn, Holger, E-mail: garn@staff.uni-marburg.de [Institute of Laboratory Medicine and Pathobiochemistry-Molecular Diagnostics, Philipps University of Marburg (Germany)

2013-10-15

The DNAzyme hgd40 was shown to effectively reduce expression of the transcription factor GATA-3 RNA which plays an important role in the regulation of Th2-mediated immune mechanisms such as in allergic bronchial asthma. However, uptake, biodistribution and pharmacokinetics of hgd40 have not been investigated yet. We examined local and systemic distribution of hgd40 in naive mice and mice suffering from experimental asthma. Furthermore, we evaluated the pharmacokinetics as a function of dose following single and repeated administration in rats and dogs. Using intranasal administration of fluorescently labeled hgd40 we demonstrated that the DNAzyme was evenly distributed in inflamed asthmatic mouse lungs within minutes after single dose application. Systemic distribution was investigated in mice using radioactive labeled hgd40. After intratracheal application, highest amounts of hgd40 were detected in the lungs. High amounts were also detected in the bladder indicating urinary excretion as a major elimination pathway. In serum, low systemic hgd40 levels were detected already at 5 min post application (p.a.), subsequently decreasing over time to non-detectable levels at 2 h p.a. As revealed by Single Photon Emission Computed Tomography, trace amounts of hgd40 were detectable in lungs up to 7 days p.a. Also in the toxicologically relevant rats and dogs, hgd40 was detectable in blood only shortly after inhalative application. The plasma pharmacokinetic profile was dose and time dependent. Repeated administration did not lead to drug accumulation in plasma of dogs and rats. These pharmacokinetic of hgd40 provide guidance for clinical development, and support an infrequent and convenient dose administration regimen. - Highlights: • Local and systemic distribution of GATA-3-specific DNAzyme hgd40 was investigated. • Pharmacokinetics of hgd40 was tested in rats and dogs. • hgd40 dissolved in PBS was easily taken up into the lungs after local application. • No

Inclusive and semi-inclusive distributions of neutral strange particles produced in π-p interactions at 4.9; 7.5 and 40 GeV/c

International Nuclear Information System (INIS)

Dzhmukhadze, S.V.; Kladnitskaya, E.N.; Popova, V. M.; Toneeva, G.P.

1978-01-01

Results on the investigation of inclusive and semi-inclusive distributions of Λ and K 0 particles, produced in π - p interactions at 4.9, 7.5 and 40 GeV/c are presen--ted. It is shown that the inclusive cross section for production of Ksup(0) mesons increases with the energy mainly in the central region, while the inclusive production of Λ hyperons increases in the fragmentation region of the target proton. The normalized structure fucntion PHIsub(x) for K 0 mesons does not achieve the scaling in the considered energy range, while the function PHIsub(x) for Λ hyperons has the scaling behaviour in a wide range of x, -0.5 < x (<=) 0.1 starting from 4.9 GeV/c

Quality of recovery after anaesthesia measured with QoR-40: a prospective observational study

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Luís Guimarães-Pereira

Full Text Available Abstract Background: QoR-40, a 40-item questionnaire on quality of recovery from anaesthesia, has been shown to measure health status after surgery. Our aim was to evaluate the incidence of poor quality of recovery in our Post Anaesthesia Care Unit and to compare their QoR-40 scores before surgery and 3 months later. Methods: A prospective observational study was conducted in adult patients consecutively admitted from 18 June to 12 July 2012. The follow-up period was 3 months. We exclude patients submitted to cardiac surgery, neurosurgery, obstetric surgery and with a mini-mental state examination test score lower than 25. The primary endpoint was quality of recovery measured with the validated Portuguese for Portugal version of the QoR-40 before surgery (T0, 24 h after surgery (T1 and 3 months after (T2. Results: A total of 114 patients completed the study. Mean QoR-40 score was 169 and patients with poor quality of recovery were identified if their QoR-40 score was lesser than 142. This occurred in 26 patients (24%. Global median scores for patients with poor quality of recovery were lower at T0 (121 vs. 184, p < 0.001, at T1 (120 vs. 177, p < 0.001 and at T2 (119 vs. 189, p < 0.001. Conclusion: Patients with poor quality of recovery had lower quality of life. This fact may allow earlier and more effective interventions, in order to improve quality of life after surgery. Beside its utility after surgery, QoR-40 may be important prior to surgery to identify patients who will develop a poor quality of recovery.

Accumulation of weathered p,p'-DDTs in hybridized Cucurbita pepo cultivars.

Science.gov (United States)

Isleyen, Mehmet; Sevim, Pinar; White, Jason C

2012-08-01

Cucurbita pepo spp pepo (zucchini) is known as an exceptional weathered dichlorodiphenyldichloroethylene (DDE) accumulator, whereas Cucurbita pepo ssp ovifera (squash) is termed a nonaccumulator. Experiments were conducted with hybridized zucchini and squash to assess the inheritance pattern of DDX (the sum of p,p'-dichlorodiphenyltrichloroethane [p,p'-DDT], p,p'-dichlorodiphenyldichloroethane [p,p'-DDD], and p,p'-dichlorodiphenyldichloroethylene [p,p'-DDE]) accumulation potential in xylem sap and tissues of parental, F1 hybrids, and F1 backcross (BC) generations of plants. Plants were grown in pots containing soil with weathered DDX at 732 to 1,130 ng/g soil or under field conditions in soil with 322 to 2,700 ng/g. The DDX stem bioconcentration factors and xylem sap values showed differences between parental and hybridized plants of squash and zucchini. For squash grown in greenhouse conditions, the DDX flow rate in the xylem sap was 17.3, 121, and 40.8 ng/h in parental, F1 hybrids, and F1 BC plants, respectively. Similarly, the stem DDX content of parental, F1, and F1 BC squash was 11, 253, and 96 ng/g (dry wt), respectively. A similar inheritance pattern for squash was observed when the plants were grown under field conditions. The DDX flow rates in the xylem sap of pot-grown parental, F1, and F1 BC zucchini cultivars were 100, 8.5, and 26 ng/hr, respectively, and the stem DDX content was 191, 102, and 142 ng/g, respectively. Again, similar trends in accumulation potential were observed for hybridized zucchini grown under field conditions. The DDX concentrations in parental plants matched the expected pattern, with hybrids midway between the two species, and the backcross being more like the parent again for both species. This inheritance pattern of contaminant accumulation and translocation ability follows classical Mendelian segregation and suggests single-gene or single-locus control. Copyright © 2012 SETAC.

40 CFR 40.125-1 - Limitations on duration.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Limitations on duration. 40.125-1 Section 40.125-1 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE RESEARCH AND DEMONSTRATION GRANTS § 40.125-1 Limitations on duration. (a) [Reserved] (b) No...

40 CFR 40.160-2 - Financial status report.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Financial status report. 40.160-2 Section 40.160-2 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE RESEARCH AND DEMONSTRATION GRANTS § 40.160-2 Financial status report. A financial status report...

High resolution emission Fourier transform infrared spectra of the 4p-5s and 5p-6s bands of ArH.

Science.gov (United States)

Baskakov, O I; Civis, S; Kawaguchi, K

2005-03-15

In the 2500-8500 cm(-1) region several strong emission bands of (40)ArH were observed by Fourier transform spectroscopy through a dc glow discharge in a mixture of argon and hydrogen. Rotational-electronic transitions of the two previously unstudied 4p-5s and 5p-6s,v = 0-0, bands of (40)ArH were measured and assigned in the 6060 and 3770 cm(-1) regions, respectively. A simultaneous fit of the emission transitions of the 4p-5s and 5p-6s bands and an extended set of transitions of the 6s-4p band observed by Dabrowski, Tokaryk, and Watson [J. Mol. Spectrosc. 189, 95 (1998)] and remeasured in the present work yielded consistent values of the spectroscopic parameters of the electronic states under investigation. In the branch of the 4p-5s band with transitions of type (Q)Q(f(3)e) we observed a narrowing in the linewidths with increasing rotational quantum number N. The rotational dependence of the linewidth is caused by predissociation of the 5s state by the repulsive ground 4s state through homogeneous coupling and changes in overlap integrals of the vibrational wave functions with the rotational level. Analysis was based on the Fermi's golden rule approximation model. In the 4p-5s band region a vibrational sequence ofv(')-v(")=1-1, 2-2, and 3-3 were recorded and a number of transitions belonging to the strongest (Q)Q(f(3)e) form branch of the 1-1 band were analyzed.

Low grade inflammation as measured by levels of YKL-40

DEFF Research Database (Denmark)

Rathcke, Camilla Noelle; Raymond, Ilan; Kistorp, Caroline

2010-01-01

, increasing YKL-40 levels independently predicted overall and CV mortality rate with hazard ratios of 1.58 (95% confidence interval (CI), 1.12-2.23, p=0.009) and 1.57 (95% CI, 1.00-2.46, p=0.049) after adjustment for age, sex, smoking, total cholesterol, hsCRP, NT-proBNP and UACR. In combined Kaplan...

Study of ln s Physics in $\\bar{p}p$ Interactions at the Split Field Magnet

CERN Multimedia

2002-01-01

This experiment uses the Split Field Magnet detector to investigate the low p^t, ``ln~s'', type of interactions that dominate the @*p cross-section. Systematic comparisons will be made to pp interactions. \\\\ \\\\ Specific areas to be studied include elastic scattering in the regions 0.05 $<$ !t! $<$ 0.8 GeV|2 and 0.8 $<$ !t! $<$ 4.0 GeV|2, and the use of a minimum bias trigger to study topological cross-sections, inclusive spectra, and two-body correlations. Some specialized triggers, run simultaneously with the high t elastic scattering trigger, are being studied. Examples are a trigger requiring Cerenkov identification in a limited region of phase space, and a trigger to select diffractively produced events.

Properties of the simian virus 40 (SV40) large T antigens encoded by SV40 mutants with deletions in gene A.

Science.gov (United States)

Cole, C N; Tornow, J; Clark, R; Tjian, R

1986-01-01

The biochemical properties of the large T antigens encoded by simian virus 40 (SV40) mutants with deletions at DdeI sites in the SV40 A gene were determined. Mutant large T antigens containing only the first 138 to 140 amino acids were unable to bind to the SV40 origin of DNA replication as were large T antigens containing at their COOH termini 96 or 97 amino acids encoded by the long open reading frame located between 0.22 and 0.165 map units (m.u.). All other mutant large T antigens were able to bind to the SV40 origin of replication. Mutants with in-phase deletions at 0.288 and 0.243 m.u. lacked ATPase activity, but ATPase activity was normal in mutants lacking origin-binding activity. The 627-amino acid large T antigen encoded by dlA2465, with a deletion at 0.219 m.u., was the smallest large T antigen displaying ATPase activity. Mutant large T antigens with the alternate 96- or 97-amino acid COOH terminus also lacked ATPase activity. All mutant large T antigens were found in the nuclei of infected cells; a small amount of large T with the alternate COOH terminus was also located in the cytoplasm. Mutant dlA2465 belonged to the same class of mutants as dlA2459. It was unable to form plaques on CV-1p cells at 37 or 32 degrees C but could form plaques on BSC-1 monolayers at 37 degrees C but not at 32 degrees C. It was positive for viral DNA replication and showed intracistronic complementation with any group A mutant whose large T antigen contained a normal carboxyl terminus. These findings and those of others suggest that both DNA binding and ATPase activity are required for the viral DNA replication function of large T antigen, that these two activities must be located on the same T antigen monomer, and that these two activities are performed by distinct domains of the polypeptide. These domains are distinct and separable from the domain affected by the mutation of dlA2465 and indicate that SV40 large T antigen is made up of at least three separate functional

Structure effects in polarization and cross sections for A(p, p’)X inelastic reactions on {sup 40}Ca and {sup 12}C nuclei at 1 GeV

Energy Technology Data Exchange (ETDEWEB)

Miklukho, O. V., E-mail: miklukho-ov@pnpi.rncki.ru; Kisselev, A. Yu., E-mail: kisselev@mail.desy.de; Amalsky, G. M.; Andreev, V. A.; Gavrilov, G. E.; Izotov, A. A.; Kozlenko, N. G.; Kravchenko, P. V.; Levchenko, M. P.; Novinskiy, D. V.; Prokofiev, A. N., E-mail: prokofiev-an@pnpi.rncki.ru; Shvedchikov, A. V.; Trush, S. I.; Zhdanov, A. A. [National Research Centre Kurchatov Institute, Petersburg Nuclear Physics Institute (Russian Federation)

2017-03-15

The polarization of secondary protons in the (p, p’) inelastic reactions on {sup 40}Ca and {sup 12}C nuclei at the initial proton energy of 1 GeV was measured over a wide range of scattered-proton momenta at a laboratory angle of Θ = 21°. The reaction cross sections were also measured. Scattered protons were detected by means of magnetic spectrometer equipped with a polarimeter based on multiwire-proportional chambers. A structure in the polarization and cross-section data, which is probably related to scattering off nucleon correlations in the nuclei involved, was observed.

Serum YKL-40 and uterine artery Doppler – a prospective cohort study, with focus on preeclampsia and small-for-gestational-age

DEFF Research Database (Denmark)

Gybel-Brask, Dorte; Høgdall, Estrid; Johansen, Julia

2014-01-01

OBJECTIVE: To test if serum YKL-40 is increased in women developing preeclampsia or small-for-gestational age fetuses. We also assessed the association between uterine artery pulsatility index, notching and serum YKL-40 levels. DESIGN: Prospective cohort study. SETTING: A primary referral unit......-week fetal growth examinations. Uterine artery Doppler was assessed and outcome was registered from medical records. MAIN OUTCOME MEASURES: Preeclampsia, hypertension, small-for-gestational age. RESULTS: Serum YKL-40 was associated with increasing maternal age (p ....0002), primiparity (p = 0.0003), and hypertension (p = 0.015). Serum YKL-40 increased from 12 to 20 weeks and decreased from 20-25 and 25-32 weeks of gestation. No association was found between preeclampsia and serum YKL-40. Small-for-gestational-age at birth was significantly associated with a 5.4% increase...

Establishment and Identification of Chinese Hamster Ovary Cell Lines with Stable Expression of Soluble CD40 Ligands

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JIANG Hua-wei

2014-09-01

Full Text Available Objective: To establish the Chinese Hamster Ovary (CHO cell lines with stable expression of soluble CD40 ligands (sCD40L. Methods: Recombinant plasmid pIRES2-EGFP-sCD40L, enzyme digestion and sequencing identification were obtained by cloning sCD40L coding sequences into eukaryotic expression vector pIRES2-EGFP from carrier pDC316-sCD40 containing sCD40L. CHO cells were transfected by electroporation, followed by screening of resistant clones with G418, after which monoclones were obtained by limited dilution assay and multiply cultured. Flow cytometer and reverted fluorescence microscope were applied to observe the expression of green fluorescent protein, while sCD40L expression was detected by polymerase chain reaction (PCR, reverse transcription-polymerase chain reaction (RT-PCR and enzyme-linked immunosorbent assay (ELISA from aspects of deoxyribose nucleic acid (DNA, messenger ribonucleic acid (mRNA and protein, respectively. CHO-sCD40L was cultured together with MDA-MB-231 cells to compare the expression changes of surface molecule fatty acid synthase (Fas by flow cytometer and observe the apoptosis of MDA-MB-231 cells after Fas activated antibodies (CH-11 were added 24 h later. Results: Plasmid pIRES2-EGFP-sCD40L was successfully established, and cell lines with stable expression of sCD40L were obtained with cloned culture after CHO cell transfection, which was named as B11. Flow cytometer and reverted fluorescence microscope showed ＞90% expression of green fluorescent protein, while PCR, RT-PCR and ELISA suggested integration of sCD40L genes into cell genome DNA, transcription of sCD40L mRNA and sCD40L protein expression being (4.5±2.1 ng/mL in the supernatant of cell culture, respectively. After co-culture of B11 and MDA-MB-231 cells, the surface Fas expression of MDA-MB-231 cells was increased from (3±1.02 % to (34.8±8.75%, while the apoptosis rate 24 h after addition of CH11 from (5.4±1.32% to (20.7±5.24%, and the differences

Cosmogenic excess of 40K and the flux of fast neutrons in meteorites

International Nuclear Information System (INIS)

Stegmann, W.; Begemann, F.

1975-01-01

Results are reported of a mass spectrometric investigation of the content and isotopic composition of potassium from the mesosiderite Emery (silicate phase) and the chondrite Elenovka (bulk). Normalized to the Nier value of 39 K/ 41 K = 13.47, the 40 K in Emery (K-content 220 +- 25 ppm) was found to be enriched by (4.03 +- 0.30)%, the potassium from Elenovka (760 +- 50 ppm) to be indistinguishable from terrestrial potassium. Evidence is presented that the excess 40 K in the silicates from Emery (Ca-content 6.06 weight %) has been produced essentially by secondary cosmic ray neutrons via the 40 Ca(n,p)-reaction. The total excess of (2.57 +- 0.39) x 10 14 40 K-atoms/gCa together with the excitation function of the 40 Ca(n,p)-reaction and the neutron flux spectrum of Arnold, Honda and Lal yields a dose of fast neutrons (2 MeV 16 neutrons/cm 2 and an average flux during the cosmic ray exposure age T = (134 +- 12) Myrs of PHI = (17.4 +- 3.1) neutrons/cm 2 sec. (orig./BJ) [de

Significance of measurement of serum fibrosis markers (HA, LN, P III P, IV C) in patients with pulmonary tuberculosis

International Nuclear Information System (INIS)

Zhou Mingxian

2006-01-01

Objective: To study the clinical significance of determination of serum fibrosis markers in patients with pulmonary tuberculosis. Methods: Serum hyaluronic acid (HA), laminin (LN), procollagen III peptide (P III P) and Type IV collagen (IV C) contents were determined with RIA in 128 patients with pulmonary tuberculosis both before and after treatment as well as in 40 controls. Results: Before treatment, the serum contents of the four markers in the patients were significantly higher than those in the controls (P 0.05). Decreases of the levels in treatment failures were not significant and the levels remained significantly higher than those in controls (P<0.01 or P<0.05). Conclusion: Determination of serum fibrosis markers levels in patients with pulmonary tuberculosis is valuable for early diagnosis as well as for evaluation of the effect of chemotherapy. (authors)

Effects of ionizing radiation on expression of P21 protein in Jurkat cell line and p21 gene in thymocytes and splenocytes of mice

International Nuclear Information System (INIS)

Ni Guanying; Wu Ning; Guo Haizhuo; Jin Shunzi

2011-01-01

Objective: To investigate the effects of ionizing radiation on the expression of P21 protein in Jurkat cell line and p21 gene in thymocytes and splenocytes of mice. Methods: Flow cytometry (FCM) was used to analyze the expression of P21 protein in Jurkat cells at 12 and 24 h after irradiation to 0, 0.5, 1.0, 2.0, 4.0, and 6.0 Gy. Real-time PCR was used to detect the expression of p21 gene in thymocytes and splenocytes of mice at 4 and 24 h after irradiation to 0, 0.5, 1.0, 2.0, 4.0, and 6.0 Gy. Multi-staining was used to analyze the micronucleus rates of Rct in bone marrow. Results: The expressions of P21 protein were increased in a dose-dependent manner during 0.5-4.0 Gy (t=-24.23 - -3.96, P<0.05), but decreased at 6.0 Gy at 12 and 24 h post-irradiation (t=-11.19, -14.50, P<0.05). The expressions of p21 gene in both thymocytes and splenocytes of mice were increased in dose-dependent manner in the range of 0-6.0 Gy (including 6.0 Gy) (t=-29.96-8.80, P<0.05), and reached to the peak at 6.0 Gy at 4 and 24 h post-irradiation (t=-11.84 - -3.42, P<0.05), except thymocytes at 4 h and 1.0 Gy post-irradiation (t=-3.42, P>0.05). Conclusions: The expressions of P21 protein and p21 gene could be increased by X-ray irradiation, which shows good dose-dependent manners in certain range of dose. (authors)

Effect of temperature of 32P uptake and translocation in varieties and a cross of japonica and indica rice

International Nuclear Information System (INIS)

Reddy, P.R.; Madhusudhan Rao, L.

1976-01-01

Three genotypes of rice, one in each of japonica, indica, and a japonica-indica cross were grown in half-Hoagland culture solution. 32 P (40 μCi/I) was added to each of these culture jars and placed at 10,20,30 and 40degC in growth chamber for a period of 1,5 and 10 h at each temperature. The actual uptake of 32 P in plant was very low at 10 and 20degC; moderately low at 40degC and very low at 30degC in japonica and the cross. The uptake was higher at 30 and 40degC in indica. This indicates their ability in uptake of fertilizer at these temperatures. The percentage of absorbed ions decreased at 10 and 20degC and increased at 30 and 40degC with increased duration in all the genotypes. The actual uptake of ions was in proportion to the absorbed ions at higher temperatures and inversely proportional at lower temperatures. The percentage of translocation of 32 P to shoot increased with time between 20 and 50degC in indica, whereas it was high only at 30 and 40degC in japonica and the cross. The translocation was low at 10degC in all the genotypes. The uptake of 32 P at different concentrations under different diurnal temperatures (20/10, 30/20 and 40/30degC) was also studied. (author)

GaAsP/InGaP HBTs grown epitaxially on Si substrates: Effect of dislocation density on DC current gain

Science.gov (United States)

Heidelberger, Christopher; Fitzgerald, Eugene A.

2018-04-01

Heterojunction bipolar transistors (HBTs) with GaAs0.825P0.175 bases and collectors and In0.40Ga0.60P emitters were integrated monolithically onto Si substrates. The HBT structures were grown epitaxially on Si via metalorganic chemical vapor deposition, using SiGe compositionally graded buffers to accommodate the lattice mismatch while maintaining threading dislocation density at an acceptable level (˜3 × 106 cm-2). GaAs0.825P0.175 is used as an active material instead of GaAs because of its higher bandgap (increased breakdown voltage) and closer lattice constant to Si. Misfit dislocation density in the active device layers, measured by electron-beam-induced current, was reduced by making iterative changes to the epitaxial structure. This optimized process culminated in a GaAs0.825P0.175/In0.40Ga0.60P HBT grown on Si with a DC current gain of 156. By considering the various GaAsP/InGaP HBTs grown on Si substrates alongside several control devices grown on GaAs substrates, a wide range of threading dislocation densities and misfit dislocation densities in the active layers could be correlated with HBT current gain. The effect of threading dislocations on current gain was moderated by the reduction in minority carrier lifetime in the base region, in agreement with existing models for GaAs light-emitting diodes and photovoltaic cells. Current gain was shown to be extremely sensitive to misfit dislocations in the active layers of the HBT—much more sensitive than to threading dislocations. We develop a model for this relationship where increased base current is mediated by Fermi level pinning near misfit dislocations.

P(o-chlorophenol–co-o-hydroxyphenol: kinetic formation studies and pH-sensor application

Directory of Open Access Journals (Sweden)

Said M. Sayyah

2017-03-01

Full Text Available Electrochemical copolymerization of o-chlorophenol (o-ClPh with o-hydroxyphenol (o-HOPh was conducted in aqueous H2SO4 using cyclic voltammetry technique at the Pt electrode. The reaction rate was found to be of the second order in the monomer concentration and first order in the acid concentration. The activation energy, enthalpy, and entropy for the copolymerization were found to be 20.20 kJ mol-1, 19.24 kJ mol-1 and -281.47 J K-1 mol-1, respectively. The obtained copolymer films show smooth feature with amorphous nature. Copolymer films adhere Pt electrode very well and show less reactivity in the H2SO4 medium. The pH sensitivity of the poly(oClPh-co-HOPh-modified electrode has been investigated potentiometrically using different polymer thicknesses. The potentiometric responses to pH change of the poly(oClPh-co-HOPh-modified electrode appeared reversible and linear in the range from pH 2-11 with a maximum sub-Nernstian potentiometric response slope of 40.7 mV/pH (30 °C. The slope became close to 56.2 mV/pH in the range from pH 4 to 9 at (30 °C. The poly(oClPh-co-HOPh-modified electrode readily responded to pH change but was not stable with time.

Measurement of pH, alkalinity and acidity in ultra-soft waters

African Journals Online (AJOL)

drinie

2001-10-04

Oct 4, 2001 ... A blend composed of the raw water, sodium chloride (to increase conductivity), and standard bicarbonate (to increase buffering capacity) was titrated with standard strong acid in two pH regions: 6.3 pH < 4.0. In both methods, total alkalinity was determined using the latter set of points, ...

Changes in the F0F1-ATPase activity of irradiated Lactobacillus acidophilus in the presence of ceftazidime at low pH

International Nuclear Information System (INIS)

Kalantaryan, V.P.; Trchounian, A.H.; Soghomonyan, D.R.

2014-01-01

The aim of this study was the investigation of the effects of low intensity electromagnetic irradiation (EMI) at the frequencies of 51.8 and 53 GHz and of antibiotic ceftazidime on the N,N'-dicyclohexylcarbodiimide (DCCD) inhibited ATPase activity of membrane vesicles of lactic acid bacteria Lactobacillus acidophilus grown at low pH (pH 4.0 or 6.5) and assayed at the same pH. It was shown that both frequencies EMI stimulated ATPase activity of L. acidophilus grown at pH 4.0, but EMI combined with ceftazidime and DCCD decreased ATPase activity at pH 4.0 and pH 6.5. It was suggested that the F 0 F 1 -ATPase might be a target for EMI even at low pH

40 CFR 721.5350 - Substituted nitrile (generic name).

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Substituted nitrile (generic name... Substances § 721.5350 Substituted nitrile (generic name). (a) Chemical substances and significant new uses subject to reporting. (1) The chemical substance identified generically as a substituted nitrile (PMN P-83...

Rho0 production and possible modification in Au+Au and p+p collisions at square root [sNN] = 200 GeV.

Science.gov (United States)

Adams, J; Adler, C; Aggarwal, M M; Ahammed, Z; Amonett, J; Anderson, B D; Arkhipkin, D; Averichev, G S; Badyal, S K; Balewski, J; Barannikova, O; Barnby, L S; Baudot, J; Bekele, S; Belaga, V V; Bellwied, R; Berger, J; Bezverkhny, B I; Bhardwaj, S; Bhati, A K; Bichsel, H; Billmeier, A; Bland, L C; Blyth, C O; Bonner, B E; Botje, M; Boucham, A; Brandin, A; Bravar, A; Cadman, R V; Cai, X Z; Caines, H; Calderón de la Barca Sánchez, M; Carroll, J; Castillo, J; Cebra, D; Chaloupka, P; Chattopadhyay, S; Chen, H F; Chen, Y; Chernenko, S P; Cherney, M; Chikanian, A; Christie, W; Coffin, J P; Cormier, T M; Cramer, J G; Crawford, H J; Das, D; Das, S; Derevschikov, A A; Didenko, L; Dietel, T; Dong, W J; Dong, X; Draper, J E; Du, F; Dubey, A K; Dunin, V B; Dunlop, J C; Dutta Majumdar, M R; Eckardt, V; Efimov, L G; Emelianov, V; Engelage, J; Eppley, G; Erazmus, B; Estienne, M; Fachini, P; Faine, V; Faivre, J; Fatemi, R; Filimonov, K; Filip, P; Finch, E; Fisyak, Y; Flierl, D; Foley, K J; Fu, J; Gagliardi, C A; Gagunashvili, N; Gans, J; Ganti, M S; Gaudichet, L; Geurts, F; Ghazikhanian, V; Ghosh, P; Gonzalez, J E; Grachov, O; Grebenyuk, O; Gronstal, S; Grosnick, D; Guertin, S M; Gupta, A; Gutierrez, T D; Hallman, T J; Hamed, A; Hardtke, D; Harris, J W; Heinz, M; Henry, T W; Heppelmann, S; Hippolyte, B; Hirsch, A; Hjort, E; Hoffmann, G W; Horsley, M; Huang, H Z; Huang, S L; Hughes, E; Humanic, T J; Igo, G; Ishihara, A; Jacobs, P; Jacobs, W W; Janik, M; Jiang, H; Johnson, I; Jones, P G; Judd, E G; Kabana, S; Kaplan, M; Keane, D; Khodyrev, V Yu; Kiryluk, J; Kisiel, A; Klay, J; Klein, S R; Klyachko, A; Koetke, D D; Kollegger, T; Kopytine, M; Kotchenda, L; Kovalenko, A D; Kramer, M; Kravtsov, P; Kravtsov, V I; Krueger, K; Kuhn, C; Kulikov, A I; Kumar, A; Kunde, G J; Kunz, C L; Kutuev, R Kh; Kuznetsov, A A; Lamont, M A C; Landgraf, J M; Lange, S; Lasiuk, B; Laue, F; Lauret, J; Lebedev, A; Lednický, R; LeVine, M J; Li, C; Li, Q; Lindenbaum, S J; Lisa, M A; Liu, F; Liu, L; Liu, Z; Liu, Q J; Ljubicic, T; Llope, W J; Long, H; Longacre, R S; Lopez-Noriega, M; Love, W A; Ludlam, T; Lynn, D; Ma, J; Ma, Y G; Magestro, D; Mahajan, S; Mangotra, L K; Mahapatra, D P; Majka, R; Manweiler, R; Margetis, S; Markert, C; Martin, L; Marx, J; Matis, H S; Matulenko, Yu A; McClain, C J; McShane, T S; Meissner, F; Melnick, Yu; Meschanin, A; Miller, M L; Milosevich, Z; Minaev, N G; Mironov, C; Mischke, A; Mishra, D; Mitchell, J; Mohanty, B; Molnar, L; Moore, C F; Mora-Corral, M J; Morozov, D A; Morozov, V; De Moura, M M; Munhoz, M G; Nandi, B K; Nayak, S K; Nayak, T K; Nelson, J M; Netrakanti, P K; Nikitin, V A; Nogach, L V; Norman, B; Nurushev, S B; Odyniec, G; Ogawa, A; Okorokov, V; Oldenburg, M; Olson, D; Paic, G; Pal, S K; Panebratsev, Y; Panitkin, S Y; Pavlinov, A I; Pawlak, T; Peitzmann, T; Perevoztchikov, V; Perkins, C; Peryt, W; Petrov, V A; Phatak, S C; Picha, R; Planinic, M; Pluta, J; Porile, N; Porter, J; Poskanzer, A M; Potekhin, M; Potrebenikova, E; Potukuchi, B V K S; Prindle, D; Pruneau, C; Putschke, J; Rai, G; Rakness, G; Raniwala, R; Raniwala, S; Ravel, O; Ray, R L; Razin, S V; Reichhold, D; Reid, J G; Renault, G; Retiere, F; Ridiger, A; Ritter, H G; Roberts, J B; Rogachevski, O V; Romero, J L; Rose, A; Roy, C; Ruan, L J; Sahoo, R; Sakrejda, I; Salur, S; Sandweiss, J; Savin, I; Schambach, J; Scharenberg, R P; Schmitz, N; Schroeder, L S; Schweda, K; Seger, J; Seyboth, P; Shahaliev, E; Shao, M; Shao, W; Sharma, M; Shestermanov, K E; Shimanskii, S S; Singaraju, R N; Simon, F; Skoro, G; Smirnov, N; Snellings, R; Sood, G; Sorensen, P; Sowinski, J; Speltz, J; Spinka, H M; Srivastava, B; Stanislaus, T D S; Stock, R; Stolpovsky, A; Strikhanov, M; Stringfellow, B; Struck, C; Suaide, A A P; Sugarbaker, E; Suire, C; Sumbera, M; Surrow, B; Symons, T J M; Szanto de Toledo, A; Szarwas, P; Tai, A; Takahashi, J; Tang, A H; Thein, D; Thomas, J H; Timoshenko, S; Tokarev, M; Tonjes, M B; Trainor, T A; Trentalange, S; Tribble, R E; Tsai, O; Ullrich, T; Underwood, D G; Van Buren, G; VanderMolen, A M; Varma, R; Vasilevski, I; Vasiliev, A N; Vernet, R; Vigdor, S E; Viyogi, Y P; Voloshin, S A; Vznuzdaev, M; Waggoner, W; Wang, F; Wang, G; Wang, G; Wang, X L; Wang, Y; Wang, Z M; Ward, H; Watson, J W; Webb, J C; Wells, R; Westfall, G D; Whitten, C; Wieman, H; Willson, R; Wissink, S W; Witt, R; Wood, J; Wu, J; Xu, N; Xu, Z; Xu, Z Z; Yamamoto, E; Yepes, P; Yurevich, V I; Yuting, B; Zanevski, Y V; Zhang, H; Zhang, W M; Zhang, Z P; Zhaomin, Z P; Zizong, Z P; Zołnierczuk, P A; Zoulkarneev, R; Zoulkarneeva, J; Zubarev, A N

2004-03-05

We report results on rho(770)(0)-->pi(+)pi(-) production at midrapidity in p+p and peripheral Au+Au collisions at sqrt[s(NN)]=200 GeV. This is the first direct measurement of rho(770)(0)-->pi(+)pi(-) in heavy-ion collisions. The measured rho(0) peak in the invariant mass distribution is shifted by approximately 40 MeV/c(2) in minimum bias p+p interactions and approximately 70 MeV/c(2) in peripheral Au+Au collisions. The rho(0) mass shift is dependent on transverse momentum and multiplicity. The modification of the rho(0) meson mass, width, and shape due to phase space and dynamical effects are discussed.

Plasma YKL-40: a new potential marker of fibrosis in patients with alcoholic cirrhosis?

DEFF Research Database (Denmark)

Johansen, J S; Møller, S; Price, P A

1997-01-01

YKL-40 is released or extracted in the hepatosplanchnic system and to localize YKL-40 in liver tissue. METHODS: Plasma YKL-40 was determined by radioimmunoassay in 25 patients with liver diseases (alcoholic cirrhosis (n = 20), chronic active hepatitis (n = 2), cirrhosis of unknown aetiology (n = 2...... with alcoholic liver disease. RESULTS: Plasma YKL-40 was significantly increased in patients with alcoholic cirrhosis (median, 523 micrograms/l; P ... with a moderate or severe degree of liver fibrosis, and immunohistochemical studies showed positive staining for YKL-40 antigen in areas of the liver biopsy with fibrosis. CONCLUSIONS: The increased plasma YKL-40 in patients with alcoholic cirrhosis may reflect the remodelling of liver fibrosis....

YKL-40 is elevated in cerebrospinal fluid from patients with purulent meningitis

DEFF Research Database (Denmark)

Ostergaard, C; Johansen, JS; Benfield, Thomas

2002-01-01

-Whitney test, P = 0.018). YKL-40 was most likely locally produced, since patients with infections of the central nervous system had CSF YKL-40 levels that were at least 10-fold higher than the corresponding levels in serum (2,033 microg/liter [470 to 11,600] versus 80 microg/liter [19 to 195]). The CSF...... neopterin level was the biochemical parameter in CSF and blood that correlated best with CSF YKL-40 levels, indicating that YKL-40 may be produced by activated macrophages within the central nervous system. In conclusion, high levels of YKL-40 in CSF are found in patients with purulent meningitis.......YKL-40, a member of the family 18 glycosyl hydrolases, is secreted by activated neutrophils and macrophages. It is a growth factor for connective tissue cells and a potent migration factor for endothelial cells and may function in inflammation and tissue remodeling. YKL-40 was determined in 134...

Quality of recovery after anaesthesia measured with QoR-40: a prospective observational study.

Science.gov (United States)

Guimarães-Pereira, Luís; Costa, Maria; Sousa, Gabriela; Abelha, Fernando

2016-01-01

QoR-40, a 40-item questionnaire on quality of recovery from anaesthesia, has been shown to measure health status after surgery. Our aim was to evaluate the incidence of poor quality of recovery in our Post Anaesthesia Care Unit and to compare their QoR-40 scores before surgery and 3 months later. A prospective observational study was conducted in adult patients consecutively admitted from 18 June to 12 July 2012. The follow-up period was 3 months. We exclude patients submitted to cardiac surgery, neurosurgery, obstetric surgery and with a mini-mental state examination test score lower than 25. The primary endpoint was quality of recovery measured with the validated Portuguese for Portugal version of the QoR-40 before surgery (T0), 24h after surgery (T1) and 3 months after (T2). A total of 114 patients completed the study. Mean QoR-40 score was 169 and patients with poor quality of recovery were identified if their QoR-40 score was lesser than 142. This occurred in 26 patients (24%). Global median scores for patients with poor quality of recovery were lower at T0 (121 vs. 184, p<0.001), at T1 (120 vs. 177, p<0.001) and at T2 (119 vs. 189, p<0.001). Patients with poor quality of recovery had lower quality of life. This fact may allow earlier and more effective interventions, in order to improve quality of life after surgery. Beside its utility after surgery, QoR-40 may be important prior to surgery to identify patients who will develop a poor quality of recovery. Copyright © 2015 Sociedade Brasileira de Anestesiologia. Published by Elsevier Editora Ltda. All rights reserved.

[Quality of recovery after anaesthesia measured with QoR-40: a prospective observational study].

Science.gov (United States)

Guimarães-Pereira, Luís; Costa, Maria; Sousa, Gabriela; Abelha, Fernando

2016-01-01

QoR-40, a 40-item questionnaire on quality of recovery from anaesthesia, has been shown to measure health status after surgery. Our aim was to evaluate the incidence of poor quality of recovery in our Post Anaesthesia Care Unit and to compare their QoR-40 scores before surgery and 3 months later. A prospective observational study was conducted in adult patients consecutively admitted from 18 June to 12 July 2012. The follow-up period was 3 months. We exclude patients submitted to cardiac surgery, neurosurgery, obstetric surgery and with a mini-mental state examination test score lower than 25. The primary endpoint was quality of recovery measured with the validated Portuguese for Portugal version of the QoR-40 before surgery (T0), 24h after surgery (T1) and 3 months after (T2). A total of 114 patients completed the study. Mean QoR-40 score was 169 and patients with poor quality of recovery were identified if their QoR-40 score was lesser than 142. This occurred in 26 patients (24%). Global median scores for patients with poor quality of recovery were lower at T0 (121 vs. 184, p<0.001), at T1 (120 vs. 177, p<0.001) and at T2 (119 vs. 189, p<0.001). Patients with poor quality of recovery had lower quality of life. This fact may allow earlier and more effective interventions, in order to improve quality of life after surgery. Beside its utility after surgery, QoR-40 may be important prior to surgery to identify patients who will develop a poor quality of recovery. Copyright © 2015 Sociedade Brasileira de Anestesiologia. Publicado por Elsevier Editora Ltda. All rights reserved.

Correlation between intravoxel incoherent motion magnetic resonance imaging derived metrics and serum soluble CD40 ligand level in an embolic canine stroke model

Energy Technology Data Exchange (ETDEWEB)

Xu, Xiao Quan; Wu, Chen Jiang; Lu, Shan Shan; Gao, Qian Qian; Zu, Qing Quan; Liu, Xing Long; Shi, Hai Bin; Liu, Sheng [Dept. of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing (China)

2017-09-15

To determine the relationship between intravoxel incoherent motion (IVIM) imaging derived quantitative metrics and serum soluble CD40 ligand (sCD40L) level in an embolic canine stroke model. A middle cerebral artery occlusion model was established in 24 beagle dogs. Experimental dogs were divided into low- and high-sCD40L group according to serum sCD40L level at 4.5 hours after establishing the model. IVIM imaging was scanned at 4.5 hours after model establishment using 10 b values ranging from 0 to 900 s/mm{sup 2}. Quantitative metrics diffusion coefficient (D), pseudodiffusion coefficient (D{sup *}), and perfusion fraction (f) of ischemic lesions were calculated. Quantitative metrics of ischemic lesions were normalized by contralateral hemisphere using the following formula: normalized D = D{sub stroke} / D{sub contralateral}. Differences in IVIM metrics between the low- and high-sCD40L groups were compared using t test. Pearson's correlation analyses were performed to determine the relationship between IVIM metrics and serum sCD40L level. The high-sCD40L group showed significantly lower f and normalized f values than the low-sCD40L group (f, p < 0.001; normalized f, p < 0.001). There was no significant difference in D{sup *}, normalized D{sup *}, D, or normalized D value between the two groups (All p > 0.05). Both f and normalized f values were negatively correlated with serum sCD40L level (f, r = −0.789, p < 0.001; normalized f, r = −0.823, p < 0.001). However, serum sCD40L level had no significant correlation with D{sup *}, normalized D{sup *}, D, or normalized D (All p > 0.05). The f value derived from IVIM imaging was negatively correlated with serum sCD40L level. f value might serve as a potential imaging biomarker to assess the formation of microvascular thrombosis in hyperacute period of ischemic stroke.

A rec(4) dup 4p inherited from a maternal inv(4)(p15q35): case report and review.

Science.gov (United States)

Garcia-Heras, Jaime; Martin, Judith

2002-05-01

A rec(4) dup 4p inherited from a maternal inv(4)(p15q35) was detected in a four-year-old girl with malformations, developmental delay, and behavioral problems that resemble those for trisomy 4p. A review of eight other liveborns with rec(4) dup 4p shows that about 40% of them also have manifestations in common with trisomy 4p, but the rest have a variable spectrum of malformations. Overall, the rec(4) dup 4p phenotype is not specific, and a diagnosis would not have been feasible without cytogenetic studies. This lack of a clinically recognizable phenotype could reflect the effects of the variable sizes of deletions of 4q, molecular differences in the break points, or the known variable expression of trisomy 4p. The fact that 79% of the recombinants in the offspring of inv(4)(p13-p15q35) carriers are rec(4) dup 4p suggests that meiotic recombination favors its generation or that rec(4) dup 4q are more lethal in utero. Copyright 2002 Wiley-Liss, Inc.

PLACENTAL INSUFFICIENCY IN PREGNANCY AFTER 40th WEEK OF GESTATION

Directory of Open Access Journals (Sweden)

Vladimir Antic

2007-12-01

Full Text Available Pregnancy after the 40th week of gestation is often a great dilemma for obstetrician in diagnostic, therapeutic and in psychological terms as well. The aim of this study was to confirm the phenomenon of placental insufficiency in pregnancy after the 40th gestation week, the modality of delivery and perinatal outcome.The study comprised 3405 deliveries in a period of one year, 391 of which were terminated after the end of the 40th gestation week, including healthy pregnant women with singleton pregnancies. Control group included healthy pregnant women delivered between the 37th and 40th gestation week.The incidence of deliveries after the 40th week of gestation is 11.48%. Non-stress test was reactive in 99.65% of women in the study group. At the same time, CST (constriction– stress test was assessed as negative in 78.67% of cases. The pathological CST was found in only 1.33% of cases. Doppler ultrasound measurements showed the increased resistance in umbilical artery flow in 3% of cases. Vacuum extraction was used for 16.62%of deliveries in the study group, and 8.73% of deliveries in the control group (χ2=23.24;p<0.001. In the study group, Caesarean section was performed in 14.58% of cases, and in control group in 9.07% (χ2=11.09; p<0.001.Placental insufficiency induced by duration of pregnancy is a rear phenomenon in uncompromised pregnancy. There was no significant difference in the morbidity and mortality rates between the study and control group.

40 CFR 261.21 - Characteristic of ignitability.

Science.gov (United States)

2010-07-01

... 130 °F; or any liquid flammable material having a vapor pressure exceeding 40 p.s.i. absolute at 100... flammable mixture or the flammable range with air is wider than 12 percent regardless of the lower limit.... (4) It is an oxidizer. An oxidizer for the purpose of this subchapter is a substance such as a...

Regulation of YKL-40 expression during genotoxic or microenvironmental stress in human glioblastoma cells

DEFF Research Database (Denmark)

Junker, Nanna; Johansen, Julia S; Hansen, Lasse T

2005-01-01

YKL-40 is a 40 kDa secreted glycoprotein belonging to the family of 'mammalian chitinase-like proteins', but without chitinase activity. YKL-40 has a proliferative effect on fibroblasts, chondrocytes and synoviocytes, and chemotactic effect on endothelium and vascular smooth muscle cells. Elevated...... material from glioblastomas patients. We investigated the expression of YKL-40 in three human malignant glioma cell lines exposed to different types of stress. Whereas a polymerase chain reaction transcript was detectable in all three cell lines, only U87 produced measurable amounts of YKL-40 protein. In U...... is attenuated by p53. In contrast, both basic fibroblast growth factor and tumor necrosing factor-alpha repressed YKL-40. These are the first data on regulation of YKL-40 in cancer cells. Diverse types of stress resulted in YKL-40 elevation, which strongly supports an involvement of YKL-40 in the malignant...

Molecular and clinical description of a girl with a 46,X,t(Y;4)(q11.2;p16)/45,X,der(4)t(Y;4)(q11.2;p16) karyotype and a small cryptic 4p subtelomeric deletion.

Science.gov (United States)

Zahed, Laila; Sismani, Carolina; Ioannides, M; Saleh, Monzer; Koumbaris, G; Kenj, Mazen; Abdallah, Amal; Ayyache, Maya; Patsalis, Philippos

2008-04-01

We report on a 13-year-old female with short stature, minimal axillary and pubic hair, no breast development, absence of uterus and ovaries, with the following karyotype on lymphocyte cultures: 46,X,t(Y;4)(q11.2;p16)[40]/45,X,der(4)t(Y;4)(q11.2;p16)[10]. Loss of the small derivative Y chromosome in 20% of the cells was also confirmed in skin fibroblast cultures. FISH analyses using Y centromere, SRY, subtelomere XpYp/XqYq, Y and 4 painting probes, confirmed the cytogenetic findings. High-resolution STS analyses using 40 markers covering the Y chromosome did not identify any deletion on the Y. However, de novo absence of the 4p subtelomeric region was noted by FISH, although this deletion was not revealed by Array-CGH at 1 Mb resolution, the last array clone being 0.35 or 1 Mb distal to the 4p FISH probe. The female phenotype of this patient must be due to the loss of the derivative Y chromosomes in some of her cells, especially the gonads, while the 4p subtelomeric deletion does not seem to contribute to her phenotype. Copyright 2008 Wiley-Liss, Inc.

YKL-40 is elevated in cerebrospinal fluid from patients with purulent meningitis

DEFF Research Database (Denmark)

Ostergaard, C; Johansen, JS; Benfield, Thomas

2002-01-01

, 663 microg/liter [range, 20 to 8,960]) and encephalitis (5,430 microg/liter [620 to 11,600]) than in patients with lymphocytic meningitis (137 microg/liter [41 to 1,865]) or patients without meningitis (167 microg/liter [24 to 630]) (Kruskal-Wallis and Dunn multiple comparison tests, P ...-Whitney test, P = 0.018). YKL-40 was most likely locally produced, since patients with infections of the central nervous system had CSF YKL-40 levels that were at least 10-fold higher than the corresponding levels in serum (2,033 microg/liter [470 to 11,600] versus 80 microg/liter [19 to 195]). The CSF...

40 CFR 721.555 - Alkyl amino nitriles (generic).

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Alkyl amino nitriles (generic). 721... Substances § 721.555 Alkyl amino nitriles (generic). (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substances identified generically as alkyl amino nitriles (PMNs P-96...

40 CFR 721.10036 - Acetaldehyde based polymer (generic).

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Acetaldehyde based polymer (generic... Specific Chemical Substances § 721.10036 Acetaldehyde based polymer (generic). (a) Chemical substance and... based polymer (PMN P-02-406) is subject to reporting under this section for the significant new uses...

40 CFR 721.10141 - Oils, ginger, zingiber purpureum.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Oils, ginger, zingiber purpureum. 721... Substances § 721.10141 Oils, ginger, zingiber purpureum. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified as oils, ginger, zingiber purpureum (PMN P-06...

40 CFR 97.40 - Trading program budget.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Trading program budget. 97.40 Section...) FEDERAL NOX BUDGET TRADING PROGRAM AND CAIR NOX AND SO2 TRADING PROGRAMS NOX Allowance Allocations § 97.40 Trading program budget. In accordance with §§ 97.41 and 97.42, the Administrator will allocate to the NOX...

Analysis of IL-12 p40 subunit gene and IFN-γ G5644A polymorphisms in Idiopathic Pulmonary Fibrosis

Directory of Open Access Journals (Sweden)

Welsh Kenneth I

2003-06-01

Full Text Available Abstract Background Genes encoding cytokine mediators are prime candidates for genetic analysis in conditions with T-helper (Th cell disease driven imbalance. Idiopathic Pulmonary Fibrosis (IPF is a predominantly Th2 mediated disease associated with a paucity of interferon-gamma (IFN-γ. The paucity of IFN-γ may favor the development of progressive fibrosis in IPF. Interleukin-12 (IL-12 plays a key role in inducing IFN-γ production. The aim of the current study was to assess whether the 1188 (A/C 3'UTR single nucleotide polymorphism (SNP in the IL-12 p40 subunit gene which was recently found to be functional and the 5644 (G/A 3' UTR SNP of the IFN-γ gene were associated with susceptibility to IPF. Methods We investigated the allelic distribution in these loci in UK white Caucasoid subjects comprising 73 patients with IPF and 157 healthy controls. The SNPs were determined using the polymerase chain reaction in association with sequence-specific primers incorporating mismatches at the 3'-end. Results Our results showed that these polymorphisms were distributed similarly in the IPF and control groups Conclusion We conclude that these two potentially important candidate gene single nucleotide polymorphisms are not associated with susceptibility to IPF.

Effect of boron oxide addition on fibre drawing, mechanical properties and dissolution behaviour of phosphate-based glass fibres with fixed 40, 45 and 50 mol% P2O5.

Science.gov (United States)

Sharmin, Nusrat; Parsons, Andrew J; Rudd, Chris D; Ahmed, Ifty

2014-11-01

Previous studies investigating manufacture of phosphate-based glass fibres from glasses fixed with P2O5 content less than 50 mol% showed that continuous manufacture without breakage was very difficult. In this study, nine phosphate-based glass formulations from the system P2O5-CaO-Na2O-MgO-B2O3 were prepared with P2O5 contents fixed at 40, 45 and 50 mol%, where Na2O was replaced by 5 and 10 mol% B2O3 and MgO and CaO were fixed to 24 and 16 mol%, respectively. The effect of B2O3 addition on the fibre drawing, fibre mechanical properties and dissolution behaviour was investigated. It was found that addition of 5 and 10 mol% B2O3 enabled successful drawing of continuous fibres from glasses with phosphate (P2O5) contents fixed at 40, 45 and 50 mol%. The mechanical properties of the fibres were found to significantly increase with increasing B2O3 content. The highest tensile strength (1200 ± 130 MPa) was recorded for 45P2O5-16CaO-5Na2O-24MgO-10B2O3 glass fibres. The fibres were annealed, and a comparison of the mechanical properties and mode of degradation of annealed and non-annealed fibres were investigated. A decrease in tensile strength and an increase in tensile modulus were observed for the annealed fibres. An assessment of the change in mechanical properties of both the annealed and non-annealed fibres was performed in phosphate-buffered saline (PBS) at 37℃ for 28 and 60 days, respectively. Initial loss of mechanical properties due to annealing was found to be recovered with degradation. The B2O3-containing glass fibres were found to degrade at a much slower rate as compared to the non-B2O3-containing fibres. Both annealed and non-annealed fibres exhibited a peeling effect of the fibre's outer layer during degradation. © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

Being 40 or younger is an independent risk factor for relapse in operable breast cancer patients: The Saudi Arabia experience

International Nuclear Information System (INIS)

Elkum, Naser; Al-Tweigeri, Taher; Dermime, Said; Ajarim, Dahish; Al-Zahrani, Ali; Alsayed, Adher; Tulbah, Asma; Al Malik, Osama; Alshabanah, Mohamed; Ezzat, Adnan

2007-01-01

Breast cancer in young Saudi women is a crucial problem. According to the 2002 annual report of Saudi National Cancer Registry, breast cancers that developed before the age of 40 comprise 26.4% of all female breast cancers comparing to 6.5% in the USA. Breast cancer in young patients is often associated with a poorer prognosis, but there has been a scarcity of published data in the Middle East population. Total of 867 breast cancer patients seen at King Faisal Specialist Hospital & Research Centre (KFSH&RC) between 1986 and 2002 were reviewed. Patients were divided in two age groups: ≤ 40 years and above 40 years. The clinicopathological characteristics and treatment outcomes were compared between younger and older age groups. Median age at presentation was 45 years. A total of 288 (33.2%) patients were aged ≤ 40 years. Hormone receptors were positive in 69% of patients 40 and 78.2% of patients above 40 (p = 0.009). There was a significantly higher incidence of grade III tumor in younger patients compared to older patients (p = 0.0006). Stage, tumor size, lymphatic/vascular invasion, number of nodes and axillary lymph node status, did not differ significantly between the two age groups. Younger patients had a greater probability of recurrence at all time periods (p = 0.035). Young age had a negative impact on survival of patients with positive axillary lymph nodes (p = 0.030) but not on survival of patients with negative lymph nodes (p = 0.695). Stage, tumor size, nodal status and hormonal receptors had negative impact on survival. Adjuvant chemotherapy was administered to 87.9% of younger and 65.6% of older patients (p < 0.0001). In terms of hormone therapy, the proportion of tamoxifen treated patients was significantly lower in young age group (p < 0.0001). No significant difference in radiation therapy between the two groups. Young age (≤ 40) is an independent risk factor for relapse in operable Saudi breast cancer patients. The fundamental biology of

Experimental results on J/psi production by πsup(+-), Ksup(+-), p and anti p incident on hydrogen at 39.5 GeV/c

International Nuclear Information System (INIS)

Corden, M.J.; Dowell, J.D.; Garvey, J.; Homer, R.J.; Jobes, M.; Kenyon, I.R.; McMahon, T.; Owen, R.C.; Sumorok, K.C.T.; Vallance, R.J.

1981-01-01

J/psi production at 40 GeV/c by πsup(+-), Ksup(+-), p and anti p incident on hydrogen has been studied and results compared with those obtained on tungsten in the same experiment. On hydrogen, J/psi cross-section ratios relative to π - have been measured to be (for xsub(F) > 0) sigma(π - ) : sigma(π + ) : sigma(anti p) : sigma(p) = 1 : (0.78 +- 0.09) : (0.83 +- 0.35) : (0.07 +- 0.04). The suppression of the proton induced cross sections shows the importance of calence quark-antiquark fusion in J/psi production at this energy (i.e., M 2 sub(J)sub(/)sub(psi)/s = 0.13). (orig.)

40 CFR 721.6200 - Fatty acid polyamine condensate, phosphoric acid ester salts.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Fatty acid polyamine condensate... New Uses for Specific Chemical Substances § 721.6200 Fatty acid polyamine condensate, phosphoric acid... substances identified as fatty acid polyamine condensate, phosphate ester salts (PMNs P-90-1984 and P-90-1985...

Identification of an N-terminal 27 kDa fragment of Mycoplasma pneumoniae P116 protein as specific immunogen in M. pneumoniae infections

Directory of Open Access Journals (Sweden)

Chourasia Bishwanath

2010-12-01

Full Text Available Abstract Background Mycoplasma pneumoniae is an important cause of respiratory tract infection and is increasingly being associated with other diseases such as asthma and extra-pulmonary complications. Considerable cross-reactivity is known to exist between the whole cell antigens used in the commercial serological testing assays. Identification of specific antigens is important to eliminate the risk of cross-reactions among different related organisms. Adherence of M. pneumoniae to human epithelial cells is mediated through a well defined apical organelle to which a number of proteins such as P1, P30, P116 and HMW1-3 have been localized, and are being investigated for adhesion, gliding and immunodiagnostic purposes. Methods A 609 bp fragment P116(N-27, corresponding to the N-terminal region of M. pneumoniae P116 gene was cloned and expressed. A C-terminal fragment P1(C-40, of P1 protein of M. pneumoniae was also expressed. Three IgM ELISA assays based on P116(N-27, P1(C-40 and (P116 (N-27 + P1(C-40 proteins were optimized and a detailed analysis comparing the reactivity of these proteins with a commercial kit was carried out. Comparative statistical analysis of these assays was performed with the SPSS version 15.0. Results The expressed P116(N-27 protein was well recognized by the patient sera and was immunogenic in rabbit. P1(C-40 of M. pneumoniae was also immunogenic in rabbit. In comparison to the reference kit, which is reported to be 100% sensitive and 75% specific, ELISA assay based on purified P116(N-27, P1(C-40 and (P116(N-27 + P1(C-40 proteins showed 90.3%, 87.1% and 96.8% sensitivity and 87.0%, 87.1% and 90.3% specificity respectively. The p value for all the three assays was found to be Conclusion This study shows that an N-terminal fragment of P116 protein holds a promise for serodiagnosis of M. pneumoniae infection. The IgM ELISA assays based on the recombinant proteins seem to be suitable for the use in serodiagnosis of acute M

TOMM40 rs2075650 May Represent a New Candidate Gene for Vulnerability to Major Depressive Disorder

Science.gov (United States)

McFarquhar, Martyn; Elliott, Rebecca; McKie, Shane; Thomas, Emma; Downey, Darragh; Mekli, Krisztina; Toth, Zoltan G; Anderson, Ian M; Deakin, JF William; Juhasz, Gabriella

2014-01-01

Evidence suggests that depression is a risk factor for dementia; however, the relationship between the two conditions is not fully understood. A novel gene (TOMM40) has been consistently associated with Alzheimer's disease (AD), but has received no attention in depression. We conducted a three-level cross-sectional study to investigate the association of the TOMM40 rs2075650 SNP with depression. We recruited a community sample of 1220 participants (571 controls, 649 lifetime depression) to complete a psychiatric background questionnaire, the Brief Symptom Inventory, and Big Five Inventory at Level-1, 243 (102 controls, 97 remitted, 44 currently depressed) to complete a face-to-face clinical interview and neuropsychological testing at Level-2 and 58 (33 controls, 25 remitted) to complete an emotional face-processing task during fMRI at Level-3. Our results indicated that the TOMM40 rs2075650 G allele was a significant risk factor for lifetime depression (p=0.00006) and, in depressed subjects, was a significant predictor of low extraversion (p=0.009). Currently depressed risk allele carriers showed subtle executive dysfunction (p=0.004) and decreased positive memory bias (p=0.021) together with reduced activity in the posterior (p(FWE)=0.045) and anterior (p(FWE)=0.041) cingulate during sad face emotion processing. Our results suggest that TOMM40 rs2075650 may be a risk factor for the development of depression characterized by reduced extraversion, impaired executive function, and decreased positive emotional recall, and reduced top-down cortical control during sad emotion processing. PMID:24549102

Transformation and radiosensitivity of human diploid skin fibroblasts transfected with SV40 T-antigen mutants defective in RB and P53 binding domains

International Nuclear Information System (INIS)

LingNah Su; Little, J.B.

1992-01-01

A series of human diploid fibroblast cell clones were developed by DNA transfection with either wild-type SV40 T-antigen (SV40T) or T-antigen mutants defective in its various functional domains. Cell clones expressing the wild-type SV40 T were significantly radioresistant as compared with clones transfected with the neo gene only (D o 192 ± 13 vs 127 ± 19). This radioresistance persisted in post-crisis, immortalized cell lines. A series of mutants with point or deletion mutations within each functionally active domain of SV40 T were also examined for their ability to alter radiosensitivity and induce morphological transformation. Cell clones transfected with T-antigen mutants defective in nuclear localization or origin binding showed increased radioresistance similar to clones transfected with wild-type T-antigen, and expressed morphological changes characteristic of SV40 T-transfected cells. (author)

Characterisation and classification of RISØ P2546 cup anemometer

DEFF Research Database (Denmark)

Friis Pedersen, Troels

2003-01-01

The characteristics of the RISØ P2546 cup anemometer were investigated in detail by wind tunnel and laboratory tests. The characteristics include accredited calibration, tilt response measurements for tilt angles between -40° to 40°, gust responsemeasurements at 8m/s and turbulence intensities...... of 10%, 16% and 23%, step response measurements at step wind speeds 3,7, 8, 11,9 and 15,2m/s, measurement of torque characteristics at 8m/s, rotor inertia measurements and measurements of friction ofbearings at temperatures -20°C to 40°C. Characteristics were fitted to a time domain cup anemometer model....... The characteristics were transformed into the CLASSCUP classification scheme, and were related to the cup anemometer requirements in the Danishcertification system and in the IEC 61400-121 Committee Draft....

Characterisation and classification of RISØ P2546 cup anemometer

DEFF Research Database (Denmark)

Friis Pedersen, Troels

2004-01-01

The characteristics of the RISØ P2546 cup anemometer were investigated in detail by wind tunnel and laboratory tests. The characteristics include accredited calibration, tilt response measurements for tilt angles between -40° to 40°, gust responsemeasurements at 8m/s and turbulence intensities...... of 10%, 16% and 23%, step response measurements at step wind speeds 3,7, 8, 11,9 and 15,2m/s, measurement of torque characteristics at 8m/s, rotor inertia measurements and measurements of friction ofbearings at temperatures -20°C to 40°C. Characteristics were fitted to a time domain cup anemometer model....... The characteristics were transformed into the CLASSCUP classification scheme, and were related to the cup anemometer requirements in the Danishcertification system and in the IEC 61400-121 Committee Draft....

40 CFR 721.10094 - Decene, branched and linear.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Decene, branched and linear. 721.10094... Substances § 721.10094 Decene, branched and linear. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified as decene, branched and linear (PMN P-03-272; CAS...

The in vitro and in vivo anti-cancer activities of a standardized quassinoids composition from Eurycoma longifolia on LNCaP human prostate cancer cells.

Directory of Open Access Journals (Sweden)

Kind Leng Tong

Full Text Available Quassinoids are a group of diterpenoids found in plants from the Simaroubaceae family. They are also the major bioactive compounds found in Eurycoma longifolia which is commonly used as traditional medicine in South East Asia to treat various ailments including sexual dysfunction and infertility. These uses are attributed to its ability to improve testosterone level in men. Chronic consumption of E. longifolia extracts has been reported to increase testosterone level in men and animal model but its effect on prostate growth remains unknown. Therefore, the present study investigates the effects of a standardized total quassinoids composition (SQ40 containing 40% of the total quassinoids found in E. longifolia on LNCaP human prostate cancer cell line. SQ40 inhibited LNCaP cell growth at IC50 value of 5.97 μg/mL while the IC50 on RWPE-1 human prostate normal cells was 59.26 μg/mL. SQ40 also inhibited 5α-dihydrotestosterone-stimulated growth in LNCaP cells dose-dependently. The inhibitory effect of SQ40 in anchorage-independent growth of LNCaP cells was also demonstrated using soft agar assay. SQ40 suppressed LNCaP cell growth via G0/G1 phase arrest which was accompanied by the down-regulation of CDK4, CDK2, Cyclin D1 and Cyclin D3 and up-regulation of p21Waf1/Cip1 protein levels. SQ40 at higher concentrations or longer treatment duration can cause G2M growth arrest leading to apoptotic cell death as demonstrated by the detection of poly(ADP-ribose polymerase cleavage in LNCaP cells. Moreover, SQ40 also inhibited androgen receptor translocation to nucleus which is important for the transactivation of its target gene, prostate-specific antigen (PSA and resulted in a significant reduction of PSA secretion after the treatment. In addition, intraperitoneal injection of 5 and 10 mg/kg of SQ40 also significantly suppressed the LNCaP tumor growth on mouse xenograft model. Results from the present study suggest that the standardized total quassinoids

Harju Elekter 40 / Andres Allikmäe ; interv. Rein Aro

Index Scriptorium Estoniae

Allikmäe, Andres, 1957-

2008-01-01

AS Harju Elekter juhatuse esimees oma ettevõtte arengust 40 aasta jooksul, üleminekust käsumajandusest turumajandusse, iseseisva väliskaubanduslitsentsi saamisest 1988. a., põhitoodetest ja tegevusvaldkondadest, ekspordist, hakkamasaamisest Eesti praeguses raskes majanduslikus olukorras

Athermal metal optics made of nickel plated AlSi40

Science.gov (United States)

Gebhardt, Andreas; Kinast, Jan; Rohloff, Ralf-Rainer; Seifert, Walter; Beier, Matthias; Scheiding, Sebastian; Peschel, Thomas

2017-11-01

Metal optics is an inherent part of space instrumentation for years. Diamond turned aluminum (Al6061) mirrors are widely used for application in the mid- and near-infrared (mid-IR and NIR, respectively) spectral range. Aluminum mirrors plated with electroless nickel (NiP) expand the field of application towards multispectral operating instruments down to the ultraviolet wavelengths. Due to the significant mismatch in the coefficient of thermal expansion (CTE) between aluminum and NiP, however, this advantage occurs at the cost of bimetallic bending. Challenging requirements can be met by using bare beryllium or aluminum beryllium composites (AlBeMet) as a CTE tailored substrate material and amorphous NiP as polishable layer. For health reasons, the use of beryllium causes complications in the process chain. Thus, the beryllium approach is subjected to specific applications only. Metal optics has proven to be advantageous in respect of using conventional CNC and ultra-precision fabrication methods to realize complex and light-weighted instrument structures. Moreover, the mirror designs can be effectively optimized for a deterministic system assembly and optimization. Limitations in terms of dimensional stability over temperature and time are mainly given by the inherent material properties (figures of merit) of the substrate material in interaction with the polishing layer. To find an optimal compromise, a thermal matched aluminum-silicon alloy (silicon contents ≍ 40 wt%) plated with NiP (AlSi40/NiP ) was investigated in a joined project of the Max Planck Institute for Astronomy MPIA and the Fraunhofer Institute for Applied Optics and Precision Engineering IOF. The main tasks of the project were the minimization of the bimetallic bending, the development of reliable stabilizing and aging procedures, and the establishment of a proven fabrication method. This paper describes fundamental results regarding the optimization of the athermal material combination

Prognostic value of p53 mutations in patients with locally advanced esophageal carcinoma treated with definitive chemoradiotherapy

Energy Technology Data Exchange (ETDEWEB)

Ito, Tomohiro; Kaneko, Kazuhiro; Makino, Reiko; Ito, Hiroaki; Konishi, Kazuo; Kurahashi, Toshinori; Kitahara, Tadashi; Mitamura, Keiji [Showa Univ., Tokyo (Japan). School of Medicine

2001-05-01

A significant correlation has been found between p53 mutation and response to chemotherapy or radiotherapy. To determine the prognostic value of p53 mutation in patients with locally advanced esophageal carcinoma treated with definitive chemoradiotherapy, p53 mutation was analyzed using the biopsied specimens taken for diagnosis. Concurrent chemoradiotherapy was performed for 40 patients with severe dysphagia caused by esophageal squamous cell carcinoma associated with T3 or T4 disease. Chemotherapy consisted of protracted infusion of 5-fluorouracil, combined with an infusion of cisplatinum. Radiation treatment of the mediastinum was administered concomitantly with chemotherapy. The p53 gene mutation was detected by fluorescence-based polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) methods. DNA sequences were determined for DNA fragments with shifted peaks by SSCP methods. Of the 40 patients, 15 had T3 disease and 25 had T4 disease; 11 patients had M1 lymph node (LYM) disease. Of the 40 patients, 13 (33%) achieved a complete response. The median survival time was 14 months, and the 2-year survival rate was 20%. Among the 40 tumor samples, p53 mutation was detected in 24 tumors (60%). The survival rate in the 24 patients with p53 mutation did not differ significantly from that in the 16 patients without p53 mutation. In contrast, the 15 patients with T3 disease survived longer than the 25 patients with T4 disease (P=0.016); however, the survival rate in the 11 patients with M1 LYM disease did not differ significantly from that in the 29 patients without M1 LYM disease. Concurrent chemoradiotherapy is potentially curative for locally advanced esophageal carcinoma, but p53 genetic abnormality has no impact on prognosis. (author)

Prognostic value of p53 mutations in patients with locally advanced esophageal carcinoma treated with definitive chemoradiotherapy

International Nuclear Information System (INIS)

Ito, Tomohiro; Kaneko, Kazuhiro; Makino, Reiko; Ito, Hiroaki; Konishi, Kazuo; Kurahashi, Toshinori; Kitahara, Tadashi; Mitamura, Keiji

2001-01-01

A significant correlation has been found between p53 mutation and response to chemotherapy or radiotherapy. To determine the prognostic value of p53 mutation in patients with locally advanced esophageal carcinoma treated with definitive chemoradiotherapy, p53 mutation was analyzed using the biopsied specimens taken for diagnosis. Concurrent chemoradiotherapy was performed for 40 patients with severe dysphagia caused by esophageal squamous cell carcinoma associated with T3 or T4 disease. Chemotherapy consisted of protracted infusion of 5-fluorouracil, combined with an infusion of cisplatinum. Radiation treatment of the mediastinum was administered concomitantly with chemotherapy. The p53 gene mutation was detected by fluorescence-based polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) methods. DNA sequences were determined for DNA fragments with shifted peaks by SSCP methods. Of the 40 patients, 15 had T3 disease and 25 had T4 disease; 11 patients had M1 lymph node (LYM) disease. Of the 40 patients, 13 (33%) achieved a complete response. The median survival time was 14 months, and the 2-year survival rate was 20%. Among the 40 tumor samples, p53 mutation was detected in 24 tumors (60%). The survival rate in the 24 patients with p53 mutation did not differ significantly from that in the 16 patients without p53 mutation. In contrast, the 15 patients with T3 disease survived longer than the 25 patients with T4 disease (P=0.016); however, the survival rate in the 11 patients with M1 LYM disease did not differ significantly from that in the 29 patients without M1 LYM disease. Concurrent chemoradiotherapy is potentially curative for locally advanced esophageal carcinoma, but p53 genetic abnormality has no impact on prognosis. (author)

pH-sensitive pHluorins as a molecular sensor for in situ monitoring of enzyme-catalyzed prodrug activation.

Science.gov (United States)

Liu, Hui; Cao, Xiaodan; Wang, Ping; Ma, Xingyuan

2017-07-01

This work examines the feasibility of using a pH-sensitive fluorescent protein as a molecular reporter for enzyme-catalyzed prodrug activation reaction. Specifically, a ratiometric pHluorins was examined for detection of the activity of horseradish peroxidase (HRP) for the activation of indole-3-acetic acid. The pHluorins and HRP were conjugated chemically, forming a biocatalyst with a self-reporting function. Results showed that the characteristic fluorescence intensity ratio of the conjugate shifted from 1.47 to 1.40 corresponding to the progress of the prodrug activation reaction. The effectiveness of applying the conjugate for inhibition of the growth of Bcap-37 cells was also demonstrated simultaneously with reaction monitoring. The results reveal a very promising approach to realizing in situ monitoring of enzyme activities based on pH shifting for enzyme-based prodrug therapy applications. © 2016 International Union of Biochemistry and Molecular Biology, Inc.

Soluble OX40L is associated with presence of autoantibodies in early rheumatoid arthritis

DEFF Research Database (Denmark)

Laustsen, Julie K; Rasmussen, Tue K; Stengaard-Pedersen, Kristian

2014-01-01

) and with methotrexate alone (n = 37). Adalimumab was discontinued after the first year, and patients were followed for additional 12 months. For comparison, sOX40 and sOX40L were measured in patients with chronic RA (cRA, n = 15) and healthy volunteers (HV, n = 34). Membrane-bound OX40 and OX40L expression on T cells......, B cells and monocytes were quantified. RESULTS: Soluble OX40 plasma levels of eRA patients were not different at the time of treatment initiation, but were significantly higher after 12 months of treatment, compared with HV or cRA patients. Soluble OX40L was significantly elevated throughout...... the first 12 months of treatment compared with HVs and patients with cRA. Adalimumab treatment did not influence sOX40 or sOX40L plasma levels. At baseline, sOX40L levels were strongly associated with the presence of anti-citrullinated protein antibodies (ACPA) (P

Long-term effect of tobacco on unstimulated salivary pH

OpenAIRE

Neeraj Grover; Jyoti Sharma; Shamindra Sengupta; Sanjeet Singh; Nishant Singh; Harjeet Kaur

2016-01-01

Aims and Objectives: The aim of this study was to analyze and compare the effects of tobacco on salivary pH between tobacco chewers, smokers and controls. Materials and Methods: A total of 60 subjects (males and females) aged 25-40 years, were divided equally into three groups: Tobacco smokers (Group A), chewers (Group B) and controls (Group C). Saliva of each subject was collected under resting condition. Salivary pH was determined using the specific salivary pH meter. Results: The mean (±st...

Cell wall pH and auxin transport velocity

Science.gov (United States)

Hasenstein, K. H.; Rayle, D.

1984-01-01

According to the chemiosmotic polar diffusion hypothesis, auxin pulse velocity and basal secretion should increase with decreasing cell wall pH. Experiments were designed to test this prediction. Avena coleoptile sections were preincubated in either fusicoccin (FC), cycloheximide, pH 4.0, or pH 8.0 buffer and subsequently their polar transport capacities were determined. Relative to controls, FC enhanced auxin (IAA) uptake while CHI and pH 8.0 buffer reduced IAA uptake. Nevertheless, FC reduced IAA pulse velocity while cycloheximide increased velocity. Additional experiments showed that delivery of auxin to receivers is enhanced by increased receiver pH. This phenomenon was overcome by a pretreatment of the tissue with IAA. Our data suggest that while acidic wall pH values facilitate cellular IAA uptake, they do not enhance pulse velocity or basal secretion. These findings are inconsistent with the chemiosmotic hypothesis for auxin transport.

Intracellular pH in human brain measured by P-31 MR spectroscopy during changes in arterial CO/sub 2/ tension

International Nuclear Information System (INIS)

Jensen, K.E.; Thomsen, C.; Henriksen, O.

1987-01-01

Six healthy volunteers were examined. A 1.5-T Siemens whole-body scanner was used. A three-turn solenoid surface coil was used. Sixty-four acquisitions with a repetition time of 6 seconds were recorded. The subjects hyperventilated and inhaled air with 5% and 7% CO/sub 2/. The breathing of air with 5% CO/sub 2/ resulted in an arterial blood tension of 40 mm Hg, and no changes in pH could be detected. Breathing of air with 7% CO/sub 2/ resulted in arterial tension of 55 mm Hg and gave a decrease of 0.1 pH unit. The spectra after 15 minutes of hyperventilation showed an increase of 0.1 pH unit

Uptake of 40K and 137Cs in native plants of the Marshall Islands.

Science.gov (United States)

Simon, S L; Graham, J C; Terp, S D

2002-01-01

Uptake of 137Cs and 40K was studied in seven native plant species of the Marshall Islands. Plant and soil samples were obtained across a broad range of soil 137Cs concentrations (0.08-3900 Bq/kg) and a narrower range of 40K soil concentrations (2.3-55 Bq/kg), but with no systematic variation of 40K relative to 137Cs. Potassium-40 concentrations in plants varied little within the range of 40K soil concentrations observed. Unlike the case for 40K, 137Cs concentrations increased in plants with increasing 137Cs soil concentrations though not precisely in a proportionate manner. The best-fit relationship between soil and plant concentrations was P = aSb where a and b are regression coefficients and P and S are plant and soil concentrations, respectively. The exponent b for 40K was zero, implying plant concentrations were a single value, while b for 137Cs varied between 0.51 and 0.82, depending on the species. For both 40K and 137Cs, we observed a decreasing concentration ratio (where concentration ratio=plant concentration/soil concentration) with increasing soil concentrations. For the CR values, the best-fit relationship was of the form CR = aSb/S = aSb(-1). For the 40K CR functions, the exponent b - 1 was close to - 1 for all species. For the 137Cs CR functions, the exponent b - 1 varied from -0.19 to -0.48. The findings presented here, aswell as those by other investigators, collectively argue against the usefulness of simplistic ratio models to accurately predict uptake of either 40K or 137Cs in plants over wide ranges of soil concentration.

Evaluation of P-Listed Pharmaceutical Residues in Empty Pharmaceutical Containers

Science.gov (United States)

Under the Resource Conservation and Recovery Act (RCRA), some pharmaceuticals are considered acute hazardous wastes because their sole active pharmaceutical ingredients are P-listed commercial chemical products (40 CFR 261.33). Hospitals and other healthcare facilities have stru...

40 CFR 721.4596 - Diazo substituted carbomonocyclic metal complex.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Diazo substituted carbomonocyclic... Specific Chemical Substances § 721.4596 Diazo substituted carbomonocyclic metal complex. (a) Chemical... as a diazo substituted carbomonocyclic metal complex (PMN P-94-1039) is subject to reporting under...

Optimization of screen-printed ruthenium dioxide electrodes for pH measurements

International Nuclear Information System (INIS)

Wyzkiewicz, I.

2002-01-01

Optimization of disposable, screen-printed pH-sensors based on ruthenium dioxide is described in this paper. The electrodes were prepared with the use of thick-film technology. The pH-sensitive layers were deposited onto polyester foil. Polymer graphite paste containing ruthenium dioxide from 0% to 90% has been investigated. The dependence of the pH-sensitive layers related to ruthenium dioxide content is presented. The investigation proved that the electrodes containing 40-60% ruthenium dioxide exhibit linear high sensitivity (∼ 50 mV/pH) in the wide range of pH (2 - 11) as well as very good reproducibility. (author)

PRAS40 is an integral regulatory component of erythropoietin mTOR signaling and cytoprotection.

Directory of Open Access Journals (Sweden)

Zhao Zhong Chong

Full Text Available Emerging strategies that center upon the mammalian target of rapamycin (mTOR signaling for neurodegenerative disorders may bring effective treatment for a number of difficult disease entities. Here we show that erythropoietin (EPO, a novel agent for nervous system disorders, prevents apoptotic SH-SY5Y cell injury in an oxidative stress model of oxygen-glucose deprivation through phosphatidylinositol-3-kinase (PI 3-K/protein kinase B (Akt dependent activation of mTOR signaling and phosphorylation of the downstream pathways of p70 ribosomal S6 kinase (p70S6K, eukaryotic initiation factor 4E-binding protein 1 (4EBP1, and proline rich Akt substrate 40 kDa (PRAS40. PRAS40 is an important regulatory component either alone or in conjunction with EPO signal transduction that can determine cell survival through apoptotic caspase 3 activation. EPO and the PI 3-K/Akt pathways control cell survival and mTOR activity through the inhibitory post-translational phosphorylation of PRAS40 that leads to subcellular binding of PRAS40 to the cytoplasmic docking protein 14-3-3. However, modulation and phosphorylation of PRAS40 is independent of other protective pathways of EPO that involve extracellular signal related kinase (ERK 1/2 and signal transducer and activator of transcription (STAT5. Our studies highlight EPO and PRAS40 signaling in the mTOR pathway as potential therapeutic strategies for development against degenerative disorders that lead to cell demise.

Population of the 3P2,1,0 fine-structure states in the 3s and 3p photoionization of atomic chlorine

International Nuclear Information System (INIS)

Krause, M.O.; Caldwell, C.D.; Whitfield, S.B.; de Lange, C.A.; van der Meulen, P.

1993-01-01

In a high-resolution photoelectron-spectrometry study of the photoionization of chlorine atoms in both the 3s and 3p subshells, we were able to resolve contributions from ionic states with specific J values and measure the relative populations of these fine-structure components. Our photoelectron spectra, recorded at hν=29.2 eV, give ratios of 3 P 2 : 3 P 1 : 3 P 0 =100:40.59.5 for 3p photoionization and 3 P 2 : 3 P 1 =100:31 for 3s photoionization. While the results for 3p ionization are in accord with predictions based on a simple geometric analysis, the contribution of the 3 P 1 state in 3s photoionization is larger than that predicted by this simple model. The geometric predictions are also compared with results from a similar measurement of the population of the 4p -1 ( 3 P J ) states produced in the 4p ionization of Br and with earlier work on the production of 3 D 2,1,0 states in d-shell photoionization of Cu and Ag

P-N semiconductor junctions used as X-ray detectors

International Nuclear Information System (INIS)

Pela, C.A.; Bruco, J.L.; Navas, E.A.; Paula, E. de; Guilardi Neto, T.

1987-01-01

The current response of some comercial P-N semiconductor junctions in function of X-ray incidency, in 40 to 140 KVp band used in diagnosis was characterized. Some junctions were also exposed to radiation of 80 to 250 KVp used in therapy. (C.G.C.) [pt

The Top Quark in Muon + Jet Events from 1.8-TeV $p\\bar{p}$ Collisions

Energy Technology Data Exchange (ETDEWEB)

Yoshikawa, Cary Yuji [Hawaii U.

1996-01-01

An optimized search for the top (or truth) quark is performed in $t\\bar{t} \\to \\mu$ + jet events produced by $p\\bar{p}$ collisions with $\\sqrt{s}$ = 1.8 TeV at the Fermilab Tevatron and observed with the D0 detector. The data set analyzed is identical to that used by the D0 Collaboration in its reported discovery of the top quark which engaged other decay channels as well.1 Four events are observed with an expected background of 1.40 $\\pm$ 0.71 events. The probability for an upward fluctuation of the background to produce the observed number of events is 0.081 (equivalent to 1.7 standard deviations). An analysis for the top quark mass is carried out based on the amount of jet activity transverse to the $p\\bar{p}$ beam direction. The resultant top quark mass value is measured to be $185^{+16}_ {-26}$(stat.)$^{+6}_ {-8}$(syst.) GeV/$c^2$ with a corresponding production cross section of 5.3 $\\pm$ 4.4 pb.

40 CFR 96.40 - State trading program budget.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 20 2010-07-01 2010-07-01 false State trading program budget. 96.40... (CONTINUED) NOX BUDGET TRADING PROGRAM AND CAIR NOX AND SO2 TRADING PROGRAMS FOR STATE IMPLEMENTATION PLANS NOX Allowance Allocations § 96.40 State trading program budget. The State trading program budget...

Standardization of 40Ar-39Ar dating facility at KDMIPE, ONGC, Dehradun

International Nuclear Information System (INIS)

Rathore, S.S.; Singh, M.P.; Vijan, A.R.; Bansal, M.; Prabhu, B.N.; Misra, K.N.

2003-01-01

In the pursuit of acquiring state of the art technology, efforts were being made for last more than two years to establish 40 Ar- 39 Ar dating facility at KDMIPE. The 40 Ar- 39 Ar dating technique is an analytical conversion of the conventional K-Ar dating method. In this method, the sample to be dated is first irradiated in a nuclear reactor to transform a portion of 39 K to 39 Ar by the fast neutron reaction i.e. 39 K(n, p) 39 Ar. After irradiation, the sample is placed in an ultra-high vacuum system and the argon extracted from it by fusion is purified and analyzed isotopically in a mass spectrometer. The relative abundances of 40 Ar, 39 Ar, 37 Ar and 36 Ar are measured. The 40 Ar/ 39 Ar K is determined, where 40 Ar is the radiogenic argon, and 39 Ar K is the 39 Ar produced from 39 K during the irradiation. The 40 Ar/ 39 Ar K ratio is proportional to the 40 Ar/ 40 K ratio in the sample and, therefore, is proportional to age

40 CFR 40.160-3 - Reporting of inventions.

Science.gov (United States)

2010-07-01

... application. (b) A final invention report is required within 90 days after completion of the project period... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Reporting of inventions. 40.160-3... ASSISTANCE RESEARCH AND DEMONSTRATION GRANTS § 40.160-3 Reporting of inventions. As provided in appendix B of...

40 CFR 721.3710 - Polyether modified fatty acids (generic).

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Polyether modified fatty acids... Specific Chemical Substances § 721.3710 Polyether modified fatty acids (generic). (a) Chemical substance... Polyether modified fatty acids (PMN P-99-0435) is subject to reporting under this section for the...

40 CFR 721.5762 - Aromatic aldehyde phenolic resin (generic).

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Aromatic aldehyde phenolic resin... Specific Chemical Substances § 721.5762 Aromatic aldehyde phenolic resin (generic). (a) Chemical substance... aromatic aldehyde phenolic resin (PMN P-01-573) is subject to reporting under this section for the...

40 CFR 721.9513 - Modified magnesium silicate polymer (generic).

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Modified magnesium silicate polymer... Specific Chemical Substances § 721.9513 Modified magnesium silicate polymer (generic). (a) Chemical... as modified magnesium silicate polymer (PMN P-98-604) is subject to reporting under this section for...

40 CFR 721.10043 - Dineopentyl-4-substituted phthalate (generic).

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Dineopentyl-4-substituted phthalate... Specific Chemical Substances § 721.10043 Dineopentyl-4-substituted phthalate (generic). (a) Chemical... as dineopentyl-4-substituted phthalate (PMN P-02-697) is subject to reporting under this section for...

40 CFR 721.1878 - Alkali metal alkyl borohydride (generic).

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Alkali metal alkyl borohydride... Specific Chemical Substances § 721.1878 Alkali metal alkyl borohydride (generic). (a) Chemical substance... alkali metal alkyl borohydride (PMN P-00-1089) is subject to reporting under this section for the...

Characterisation and classification of RISOe P2546 cup anemometer

Energy Technology Data Exchange (ETDEWEB)

Friis Pedersen, T.

2003-04-01

The characteristics of the RISOe P2546 cup anemometer were investigated in detail by wind tunnel and laboratory tests. The characteristics include accredited calibration, tilt response measurements for tilt angles between -40 degC to 40 degC, gust response measurements at 8m/s and turbulence intensities of 10%, 16% and 23%, step response measurements at step wind speeds 3,7, 8, 11,9 and 15,2m/s, measurement of torque characteristics at 8m/s, rotor inertia measurements and measurements of friction of bearings at temperatures -20 degC to 40 degC. Characteristics were fitted to a time domain cup anemometer model. The characteristics were transformed into the CLASSCUP classification scheme, and were related to the cup anemometer requirements in the Danish certification system and in the IEC 61400-121 Committee Draft. (au)

Biological effects of brachytherapy using a 32P-patch on the skin of Sencar mice

International Nuclear Information System (INIS)

Salgueiro, M.J.; Collia, N.; Duran, H.; Palmieri, M.; Medina, V.; Ughetti, R.; Nicolini, J.; Zubillaga, M.

2009-01-01

In recent years, specially designed patches containing beta emitters have been developed for contact brachytherapy of skin lesions. The aim of the present work was to evaluate the biological effects of the 32 P-patch on the skin of Sencar mice as a result of a brachytherapy treatment. For this purpose, a 32 P-patch was prepared with Chromic 32 P-phosphate and silicone and the classical model of two-stage skin carcinogenesis was reproduced in Sencar mice. Animals were divided in six groups. Four groups received the contact brachytherapy treatments using a scheme of a single session of 40 and 60 Gy (SD40 and SD60) and a scheme of two sessions of 40 and 60 Gy each (FD40 and FD60). The other two groups were used as controls of the single (CSD) and the fractionated (CFD) treatments. Radiation doses were estimated with equations derived from the MIRD DOSE scheme, and biologically effective doses (BED) were calculated according to equations derived from the linear-quadratic model. The endpoint to evaluate the treatments effects was tumor size after a follow-up period of 44 days. Finally, animals were sacrificed in order to get samples of all tumors for histological analysis and PCNA staining. Erythema, dermatitis and skin ulceration developed in almost all treated animals, but they gradually healed with regeneration of tissue during the follow-up period. Radiation effects on the skin of SD40, SD60, FD40 and FD60 showed a significant reduction of the tumor size with regard to controls, independently of the scheme and the radiation dose considered. PCNA staining scores of control groups were higher than for treated groups, independently of the scheme and the radiation dose considered. This radioactive 32 P-silicone-patch which is easy to prepare and use in the treatment of skin diseases, seems promising as a radioactive device for clinical use.

Dose-dependent suppression by ethanol of transient auditory 40-Hz response.

Science.gov (United States)

Jääskeläinen, I P; Hirvonen, J; Saher, M; Pekkonen, E; Sillanaukee, P; Näätänen, R; Tiitinen, H

2000-02-01

Acute alcohol (ethanol) challenge is known to induce various cognitive disturbances, yet the neural basis of the effect is poorly known. The auditory transient evoked gamma-band (40-Hz) oscillatory responses have been suggested to be associated with various perceptual and cognitive functions in humans; however, alcohol effects on auditory 40-Hz responses have not been investigated to date. The objective of the study was to test the dose-related impact of alcohol on auditory transient evoked 40-Hz responses during a selective-attention task. Ten healthy social drinkers ingested, in four separate sessions, 0.00, 0. 25, 0.50, or 0.75 g/kg of 10% (v/v) alcohol solution. The order of the sessions was randomized and a double-blind procedure was employed. During a selective attention task, 300-Hz standard and 330-Hz deviant tones were presented to the left ear, and 1000-Hz standards and 1100-Hz deviants to the right ear of the subjects (P=0. 425 for each standard, P=0.075 for each deviant). The subjects attended to a designated ear, and were to detect the deviants therein while ignoring tones to the other ear. The auditory transient evoked 40-Hz responses elicited by both the attended and unattended standard tones were significantly suppressed by the 0.50 and 0.75 g/kg alcohol doses. Alcohol suppresses auditory transient evoked 40-Hz oscillations already with moderate blood alcohol concentrations. Given the putative role of gamma-band oscillations in cognition, this finding could be associated with certain alcohol-induced cognitive deficits.

A Cross-Strait Comparison of Innovation Policy under Industry 4.0 and Sustainability Development Transition

OpenAIRE

Kuan Chung Lin; Joseph Z. Shyu; Kun Ding

2017-01-01

While the advent of Industry 4.0 is emblematic of national strategy for industrial revitalization, the proliferation of technology has drastically changed the landscape of many major sectors of global industries. Industry 4.0 encompasses multi-dimensional concepts—including computerization, digitization, and intelligentization—of business operations based on cyber-physical-systems (CPS) and the Internet-of-Things (IoTs). The vision of Industry 4.0 will bring about improvements in industrial p...

Frede P. Jensen og den danske indkredsning af Vasatidens Sverige 40 år efter

DEFF Research Database (Denmark)

Andersen, Dan H.

2016-01-01

Artiklen er en analyse af receptionen af Frede P. Jensens kritik af den svenske historiker Arthur Stilles indflydelsesrige teori fra 1918 om årsagerne den svenske stormagts opståen. Stilles teori så den svenske politik som et grundlæggende defensivt og eksistentielt svar på en dansk indkredsning af...

Optimization of irinotecan chronotherapy with P-glycoprotein inhibition

Energy Technology Data Exchange (ETDEWEB)

Filipski, Elisabeth; Berland, Elodie [INSERM, U776 “Rythmes biologiques et cancers”, CAMPUS CNRS, 7 rue Guy Môquet, F-94801 Villejuif (France); Univ Paris-Sud, UMR-S0776, Orsay F-91405 (France); Ozturk, Narin [INSERM, U776 “Rythmes biologiques et cancers”, CAMPUS CNRS, 7 rue Guy Môquet, F-94801 Villejuif (France); Univ Paris-Sud, UMR-S0776, Orsay F-91405 (France); Istanbul University Faculty of Pharmacy, Department of Pharmacology, Beyazit TR-34116, Istanbul (Turkey); Guettier, Catherine [Assistance Publique-Hôpitaux de Paris, Unité de Chronothérapie, Département de Cancérologie, Hôpital Paul Brousse, Villejuif F-94807 (France); Horst, Gijsbertus T.J. van der [Department of Genetics, Erasmus University Medical Center, 3000 CA Rotterdam (Netherlands); Lévi, Francis [INSERM, U776 “Rythmes biologiques et cancers”, CAMPUS CNRS, 7 rue Guy Môquet, F-94801 Villejuif (France); Univ Paris-Sud, UMR-S0776, Orsay F-91405 (France); Assistance Publique-Hôpitaux de Paris, Unité de Chronothérapie, Département de Cancérologie, Hôpital Paul Brousse, Villejuif F-94807 (France); and others

2014-02-01

The relevance of P-glycoprotein (P-gp) for irinotecan chronopharmacology was investigated in female B6D2F{sub 1} mice. A three-fold 24 h change in the mRNA expression of Abcb1b was demonstrated in ileum mucosa, with a maximum at Zeitgeber Time (ZT) 15 (p < 0.001). No rhythm was found for abcb1a in ileum mucosa, or for Abcb1a/b in Glasgow osteosarcoma (GOS), a mouse tumor cell line moderately sensitive to irinotecan. Non-tumor-bearing mice received irinotecan (50 mg/kg/day i.v. × 4 days) as a single agent or combined with P-gp inhibitor PSC833 (6.25 mg/kg/day i.p. × 4 days) at ZT3 or ZT15, respectively corresponding to the worst or the best irinotecan tolerability. Endpoints involved survival, body weight change and hematologic toxicity. Antitumor efficacy was studied in GOS-bearing mice receiving irinotecan (25, 30 or 40 mg/kg/day × 4 days) and +/− PSC833 at ZT3 or ZT15, with survival, body weight change, and tumor growth inhibition as endpoints. Non-tumor bearing mice lost an average of 17% or 9% of their body weight according to irinotecan administration at ZT3 or ZT15 respectively (p < 0.001). Dosing at ZT15 rather than ZT3 reduced mean leucopenia (9% vs 53%; p < 0.001). PSC833 aggravated irinotecan lethal toxicity from 4 to ∼ 60%. In tumor-bearing mice, body weight loss was ∼ halved in the mice on irinotecan or irinotecan–PSC833 combination at ZT15 as compared to ZT3 (p < 0.001). PSC833–irinotecan at ZT15 increased tumor inhibition by ∼ 40% as compared to irinotecan only at ZT15. In conclusion, P-gp was an important determinant of the circadian balance between toxicity and efficacy of irinotecan. - Highlights: • Irinotecan chronotolerance and chronoefficacy change as drug was applied with PSC833. • P-glycoprotein is an important player of the toxicity and efficacy of irinotecan. • Timing should be considered if chemotherapy is performed with a MDR1 inhibitor.

Intracellular pH homeostasis in Leishmania donovani amastigotes and promastigotes

International Nuclear Information System (INIS)

Glaser, T.A.; Baatz, J.E.; Kreishman, G.P.; Mukkada, A.J.

1988-01-01

Intracellular pH and pH gradients of Leishmania donovani amastigotes and promastigotes were determined over a broad range of extracellular pH values. Intracellular pH was determined by 31 P NMR and by equilibrium distribution studies with 5,5-dimethyloxazolidine-2,4-dione or methylamine. Promastigotes maintain intracellular pH values close to neutral between extracellular pH values of 5.0 and 7.4. Amastigote intracellular pH is maintained close to neutral at external pH values as low as 4.0. Both life stages maintain a positive pH gradient to an extracellular pH of 7.4, which is important for active transport of substrates. Treatment with ionophores, such as nigericin and carbonyl cyanide m-chlorophenylhydrazone and the ATPase inhibitor dicyclohexylcarbodiimide, reduced pH gradients in both stages. Maintenance of intracellular pH in the physiologic range is especially relevant for the survival of the amastigote in its acidic in vivo environment

(p,γ) resonance strengths in the s-d shell

International Nuclear Information System (INIS)

Paine, B.M.; Sargood, D.G.

1979-01-01

The strengths of selected resonances in the energy range 0.5-2.0 MeV in the (p,γ) reactions on 26 Mg, 30 Si, 34 S, 37 C1, 39 K and 40 Ca have been found relative to the 632 and 992 keV resonances in 27 A1(p,γ) 28 Si by relative yield measurements. Absolute measurements were conducted on the selected resonances in 27 A1(p,γ) 28 Si and 30 Si(p,γ) 31 p by semi-thick target and thin target techniques with the target thickness, needed for the latter technique, found by Rutherford backscattering of protons. Absolute strengths for all of the resonances treated, together with one from each of 23 Na, 31 p and 35 C1, reported in a previous paper, were deduced by normalizing to the absolute measurements on the 27 A1(p,γ) 28 Si resonances

Measurements of (p,γ) resonance strengths in the s-d shell

NARCIS (Netherlands)

Engelbertink, G.A.P.; Endt, P.M.

1966-01-01

Resonace strengths of selected resonances in the Ep=0.3−2.1 MeV region in the (p, γ) reactions on 23Na, 24–24Mg, 27Al, 28–30Si, 31P, 32, 34S, 35, 37Cl, 39, 41K and 40Ca are compared through relative yield measurements, using targets of many different chemical compounds, each containing at least two

Asymptotic theorems and π-p → π0n polarization

International Nuclear Information System (INIS)

Gauron, P.

1984-09-01

The surprising results of the recent 40 GeV/c Serpukhov measurement of the polarization in π - p → π 0 n are shown to support the conjecture that hadronic amplitudes may grow as fast as they are permitted to by general principles

Effect of Ni and noble metals (Ru, Pd and Pt) on performance of bifunctional MoP/SiO2 for hydroconversion of methyl laurate

Science.gov (United States)

Nie, Ziyang; Zhang, Zhena; Chen, Jixiang

2017-10-01

SiO2 supported bifunctional MoP catalysts modified with different metal promoters (Ni, Ru, Pd, Pt), where Mo/Ni and Mo/M(M = Ru, Pd and Pt) atomic ratios was respectively 10 and 40, were prepared by TPR method from the phosphate precursors. It was found that the introduction of metal promoters facilitated the reduction of phosphate precursor and enhanced the dispersion of MoP. However, the MoP catalyst acidity was scarcely influenced by the small amount of metal promoters. In the hydroconversion of methyl laurate, the promoters enhanced the MoP catalyst activity for conversion of methyl laurate and hydrogenation of alkenes (intermediate), but reduced isomerization ability. Among the promoters, Ru was an optimum to decrease selectivity to alkenes while maintain high selectivity to iso-alkanes, and Mo40RuP showed better stability than MoP. At 380 °C and 3.0 MPa, the conversion of methyl laurate, the total selectivity to C11 and C12 hydrocarbons and the selectivity to iso-alkanes maintained at 100%, ∼94% and ∼30% on Mo40RuP during 102 h, respectively. The good stability of Mo40RuP is ascribed to that the presence of Ru prevented the sintering of MoP particles and suppressed carbon deposition.

The HTLV-I tax protein transcriptionally modulates OX40 antigen expression.

Science.gov (United States)

Pankow, R; Dürkop, H; Latza, U; Krause, H; Kunzendorf, U; Pohl, T; Bulfone-Paus, S

2000-07-01

OX40 is a member of the TNF receptor family, expressed on activated T cells. It is the only costimulatory T cell molecule known to be specifically up-regulated in human T cell leukemia virus type-I (HTLV-I)-producing cells. In a T cell line, OX40 surface expression was shown to be induced by HTLV-I Tax alone. To understand molecular mechanisms of OX40 gene regulation and modulation by HTLV-I Tax, we have cloned the human OX40 gene and analyzed its 5'-flanking region. By reporter gene analysis with progressive 5' deletions from nucleotides -1259 to -64, we have defined a 157-bp DNA fragment as a minimal promoter for constitutive expression. In addition, we show that in the OX40+ cell line, Co, Tax is able to further increase OX40 surface expression. Up-regulation of OX40 promoter activity by Tax requires two upstream NF-kappaB sites, which are not active in the constitutive OX40 expression. Their deletion abrogates Tax responsiveness in reporter gene analysis. The site-directed mutagenesis of each NF-kappaB site demonstrates that cooperative NF-kappaB binding is a prerequisite for Tax-directed activity as neither site alone is sufficient for a full Tax responsiveness of the OX40 promoter. Upon Tax expression, both sites bind p65 and c-Rel. These data provide new insight into the direct regulation of OX40 by Tax and add to our understanding of the possible role of the OX40/OX40 ligand system in the proliferation of HTLV-I+ T cells.

Detection of polyomavirus simian virus 40 tumor antigen DNA in AIDS-related systemic non-Hodgkin lymphoma

Science.gov (United States)

Vilchez, Regis A.; Lednicky, John A.; Halvorson, Steven J.; White, Zoe S.; Kozinetz, Claudia A.; Butel, Janet S.

2002-01-01

Systemic non-Hodgkin lymphoma (S-NHL) is a common malignancy during HIV infection, and it is hypothesized that infectious agents may be involved in the etiology. Epstein-Barr virus DNA is found in <40% of patients with AIDS-related S-NHL, suggesting that other oncogenic viruses, such as polyomaviruses, may play a role in pathogenesis. We analyzed AIDS-related S-NHL samples, NHL samples from HIV-negative patients, peripheral blood leukocytes from HIV-infected and -uninfected patients without NHL, and lymph nodes without tumors from HIV-infected patients. Specimens were examined by polymerase chain reaction analysis with use of primers specific for an N-terminal region of the oncoprotein large tumor antigen ( T-ag ) gene conserved among all three polyomaviruses (simian virus 40 [SV40], JC virus, and BK virus). Polyomavirus T-ag DNA sequences, proven to be SV40-specific, were detected more frequently in AIDS-related S-NHL samples (6 of 26) than in peripheral blood leukocytes from HIV-infected patients (6 of 26 vs. 0 of 69; p =.0001), NHL samples from HIV-negative patients (6 of 26 vs. 0 of 10; p =.09), or lymph nodes (6 of 26 vs. 0 of 7; p =.16). Sequences of C-terminal T-ag DNA from SV40 were amplified from two AIDS-related S-NHL samples. Epstein-Barr virus DNA sequences were detected in 38% (10 of 26) AIDS-related S-NHL samples, 50% (5 of 10) HIV-negative S-NHL samples, and 57% (4 of 7) lymph nodes. None of the S-NHL samples were positive for both Epstein-Barr virus DNA and SV40 DNA. Further studies of the possible role of SV40 in the pathogenesis of S-NHL are warranted.

The influence of house dust mite sublingual immunotherapy on the TSLP-OX40L signaling pathway in patients with allergic rhinitis.

Science.gov (United States)

Meng, Qingxiang; Liu, Xiaolong; Li, Peng; He, Long; Xie, Jinghua; Gao, Xionghui; Wu, Xiaozhong; Su, Fang; Liang, Yong

2016-08-01

This study aimed to investigate the clinical efficacy of sublingual immunotherapy (SLIT) with house dust mite (HDM) extract and to examine T helper 2 (Th2)-type immune responses mediated by the thymic stromal lymphopoietin (TSLP-OX40L) signaling pathway in patients with moderate to severe allergic rhinitis (AR) after 12-month HDM SLIT. Forty-six cases of HDM-sensitized patients with persistent AR in southern China were enrolled in this study. Clinical efficacy of SLIT was assessed by determining the individual nasal symptom score (INSS) and total nasal symptom score (TNSS) after 12-month HDM SLIT. Moreover, the TSLP-OX40L signaling pathway was investigated through measurements of TSLP by enzyme-labeled immunosorbent assay (ELISA) and OX40L by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and flow cytometry. After 12 months of HDM SLIT, TNSS and INSS were significantly decreased overall compared with baseline values (p < 0.001). By the end of the 12-month HDM SLIT, TNSS had declined by ∼50% compared with baseline, and the corresponding level of TSLP in nasal lavage decreased significantly (p < 0.05). The level of OX40L messenger RNA (mRNA) in blood was markedly decreased significantly after 12-month HDM SLIT compared with baseline (t = 12.300, p < 0.05). Furthermore, significant decreases in OX40L expression on the surface of peripheral blood mononuclear cells (PBMCs) (t = 13.100, p < 0.05) and OX40L expression on the surface of CD11c+CD86+ cells in PBMCs (t = 9.946, p < 0.05) after 12-month HDM SLIT were observed. HDM SLIT downregulated Th2-type immune responses mediated by the TSLP-OX40L signaling pathway in patients with persistent moderate to severe AR. © 2016 ARS-AAOA, LLC.

Effects of p-chlorophenoxyisobutyric acid, arabinogalactan, and activated charcoal on microspore embryogenesis in kale.

Science.gov (United States)

Niu, R Q; Zhang, Y; Tong, Y; Liu, Z Y; Wang, Y H; Feng, H

2015-04-27

To improve embryogenesis in microspore cultures of kale (Brassica oleracea L. var. acephala DC.), 6-benzylaminopurine (6-BA), naphthaleneacetic acid (NAA), arabinogalactan (AG), p-chlorophenoxyisobutyric acid (PCIB), and activated charcoal (AC) were added to the medium using four varieties of kale. The results showed that the addition of AG (0.1-0.2 g/L), AC (0.1-0.2 g/L) or a combination of 6-BA (0.1-0.2 mg/L) and NAA (0.1-0.2 mg/L) promoted embryo-genesis. Adding 40 μM PCIB or a combination of 40 μM PCIB and 0.2 g/L AC to NLN-13 medium at pH 5.8 effectively enhanced embryogenesis. Treatment with a combination of 40 μM PCIB and 10 mg/L AG gave the highest rate of embryonic induction, especially in genotype "Y007," which showed a twelve-fold increase in yield.

DRD2 A1 allele and P300 abnormalities in obesity

Energy Technology Data Exchange (ETDEWEB)

Blum, K. [Univ. of Texas Health Science Center, San Antonio, TX (United States)]|[PATH Foundation, Princeton, NJ (United States); Wood, R.; Sheridan, L.P.J. [Univ. of Texas Health Science Center, San Antonio, TX (United States)] [and others

1994-09-01

Obesity is a heterogeneous and prevalent disorder having both inheritable and environmental components. The role of the dopamine system in P300 has been implicated. We genotyped 193 neuropsychiatrically ill patients with and without comorbid drug and alcohol/abuse/dependence and obesity for the prevalence of the A1 allele of the DRD2 gene. We found a significant linear trend ({chi}{sup 2} = 40.4, df=1, p<0.00001) where the percent prevalence of the A1 increased with increasing polysubstance abuse. Where the A1 allele was found in 44% of 40 obese subjects, the A1 allele prevalence was found in as much as 91% of 11 obese subjects with comorbid polysubstance abuse. 53 obese subjects having a mean body weight (BMI) of 34.6{+-}8.2 were mapped for brain electrical activity and compared with 15 controls with a BMI of 22.3{+-}3.0 (P<.001). The P3 amplitude was significantly different (two tailed; t=3.24, df=16.2, P = 0.005), whereas P3 latency was not significant. Preliminarily, we found a significant decreased P3 amplitude correlated with parental polysubstance abuse (p=0.4) with prolongation of P3 latency correlated with the three risk factors of parental substance abuse, chemical dependency and carbohydrate bingeing (P<0.02). Finally, in a small sample, the A1 allele was present in 25% of probands having 0 risk compared to 66% in those obese subjects with any risk. This work represents the first electrophysiological data to implicate P3 abnormalities in a subset of obesity and further confirms an association of the DRD2 gene and a electrophysiological marker previously indicated to have predictive value in vulnerability to addictive behaviors.

Spetsialistid soovitavad kesklinnas piirata sõidukite kiirust 40 km/h-ni / Askur Alas

Index Scriptorium Estoniae

Alas, Askur, 1973-

2004-01-01

Liiklusspetsialistid soovitavad inimvigastustega õnnetuste vähendamiseks piirata Tallinna kesklinnas kiirust 40 km/h-ni. Linna liiklusteenistus ei pea seda põhjendatuks. Lisa: Helsingi positiivne kogemus

File list: InP.Emb.50.AllAg.Mitotic_cycle_14 [Chip-atlas[Archive

Lifescience Database Archive (English)

Full Text Available InP.Emb.50.AllAg.Mitotic_cycle_14 dm3 Input control Embryo Mitotic cycle 14 SRX6451...40,SRX750075,SRX645139 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/InP.Emb.50.AllAg.Mitotic_cycle_14.bed ...

The radiosensitizing effect of Ku70/80 knockdown in MCF10A cells irradiated with X-rays and p(66)+Be(40) neutrons.

Science.gov (United States)

Vandersickel, Veerle; Mancini, Monica; Slabbert, Jacobus; Marras, Emanuela; Thierens, Hubert; Perletti, Gianpaolo; Vral, Anne

2010-04-27

A better understanding of the underlying mechanisms of DNA repair after low- and high-LET radiations represents a research priority aimed at improving the outcome of clinical radiotherapy. To date however, our knowledge regarding the importance of DNA DSB repair proteins and mechanisms in the response of human cells to high-LET radiation, is far from being complete. We investigated the radiosensitizing effect after interfering with the DNA repair capacity in a human mammary epithelial cell line (MCF10A) by lentiviral-mediated RNA interference (RNAi) of the Ku70 protein, a key-element of the nonhomologous end-joining (NHEJ) pathway. Following irradiation of control and Ku-deficient cell lines with either 6 MV X-rays or p(66)+Be(40) neutrons, cellular radiosensitivity testing was performed using a crystal violet cell proliferation assay. Chromosomal radiosensitivity was evaluated using the micronucleus (MN) assay. RNAi of Ku70 caused downregulation of both the Ku70 and the Ku80 proteins. This downregulation sensitized cells to both X-rays and neutrons. Comparable dose modifying factors (DMFs) for X-rays and neutrons of 1.62 and 1.52 respectively were obtained with the cell proliferation assay, which points to the similar involvement of the Ku heterodimer in the cellular response to both types of radiation beams. After using the MN assay to evaluate chromosomal radiosensitivity, the obtained DMFs for X-ray doses of 2 and 4 Gy were 2.95 and 2.66 respectively. After neutron irradiation, the DMFs for doses of 1 and 2 Gy were 3.36 and 2.82 respectively. The fact that DMFs are in the same range for X-rays and neutrons confirms a similar importance of the NHEJ pathway and the Ku heterodimer for repairing DNA damage induced by both X-rays and p(66)+Be(40) neutrons. Interfering with the NHEJ pathway enhanced the radiosensitivity of human MCF10A cells to low-LET X-rays and high-LET neutrons, pointing to the importance of the Ku heterodimer for repairing damage induced by both

Measurement of isovector giant quadrupole resonance in 40Ca

International Nuclear Information System (INIS)

Sims, D.A.; Thompson, M.N.; Rassool, R.; Adler, J.O.; Andersson, B.E.; Hansen, K.; Issaksson, L.; Nilsson, B.; Ruijter, H.; Schroeder, B.; Annand, J.R.M.; McGeorge, J.C.; Crawford, G.I.; Miller, G.J.

1997-01-01

The 40 Ca(γ,n) reaction was measured using tagged photons in the energy range 25-50 MeV. Neutrons were detected using two 9-element, liquid scintillator, neutron detectors placed at angles of 55 deg and 125 deg at flight path of 3.2 m. The absolute cross section was determined relative to that for D (γ,n)p, which was measured using a heavy water target. The forward/backward asymmetry in the 40 Ca (γ, n) cross section, resulting from E1/E2 interference has been used to locate and parametrize the isovector giant quadrupole resonance (IVQR). 6 refs., 2 figs

23P excitation of helium by electron impact

International Nuclear Information System (INIS)

Roeder, J.; Ehrhardt, H.; Bray, I.; Fursa, D.

1996-03-01

We present highly accurate relative measurements of 23P excitation of helium by electron impact at 30, 40 and 50 eV. These, when normalised to the convergent close-coupling (CCC) theory at one angle, yield excellent agreement at the remaining angles. This resolves some of the outstanding discrepancies between the CCC theory and earlier measurements. (authors). 14 refs., 3 figs

Bio-recovery of N and P from an anaerobic digester effluent: The ...

African Journals Online (AJOL)

The results obtained indicated that duckweed was capable of growing on the anaerobic digester effluent provided its TAN content did not exceed 42 mg N l-1. Nitrogen uptake by the duckweed from the effluent ranged between 53 and 115.7 mg l-1 whereas P uptake varied from 1.40 to 8.4 mg P l-1. The relative growth rate ...

Effect of temperature and pH on ethanol production by a putative ...

African Journals Online (AJOL)

Ojes PC2

2013-02-06

Feb 6, 2013 ... 2Department of Food Science and Technology, Rivers State University of Science and Technology,. Nkpolu, P.M.B 5080, Port Harcourt, Rivers ... As the world advances more and more in science and .... The amounts of ethanol produced at various temperature levels. pH. 5.0. 4.5. 4.0. 3.5. 3.0. 2.5. 2.0. 1.5.

40 CFR 721.3810 - Formaldehyde, polymers with substituted phenols (generic).

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Formaldehyde, polymers with... New Uses for Specific Chemical Substances § 721.3810 Formaldehyde, polymers with substituted phenols... identified generically as Formaldehyde, polymers with substituted phenols (PMN P-99-0558) is subject to...

Reflux parameters as modified by laparoscopic fundoplication in 40 patients with heartburn/regurgitation persisting despite PPI therapy: a study using impedance-pH monitoring.

Science.gov (United States)

Frazzoni, Marzio; Conigliaro, Rita; Melotti, Gianluigi

2011-04-01

Patients with typical reflux symptoms (heartburn/regurgitation) persisting despite proton pump inhibitor (PPI) therapy are not uncommon. Impedance-pH monitoring detects gastroesophageal reflux at all pH levels and may establish if ongoing symptoms on PPI therapy are associated with acid/nonacid reflux. Laparoscopic fundoplication is a therapeutic option in such patients but reflux parameters on PPI therapy and after intervention and their relationship with symptom persistence/remission have been scarcely studied. The aim of this study was to assess reflux parameters and their relationship with symptoms before and after laparoscopic fundoplication, on and off PPI therapy, respectively, in patients with PPI-unresponsive heartburn/regurgitation and with a positive symptom-reflux association and/or abnormal reflux parameters detected on PPI therapy. Impedance-pH monitoring was performed on high-dose PPI therapy and 3 months after laparoscopic fundoplication, off PPI therapy, in 40 patients with PPI-unresponsive heartburn/regurgitation. Symptoms were scored by a validated questionnaire. Esophageal acid exposure time as well as the number of total and proximal reflux events and of acid and weakly acidic refluxes decreased significantly after surgery: normal values were found in 100, 77, 95, 92 and 65% of cases, respectively. Weakly alkaline refluxes increased significantly postoperatively but neither before nor after intervention were associated with symptoms. All patients reported total/subtotal remission of heartburn/regurgitation 3 months after surgery. Laparoscopic fundoplication improves acid and weakly acidic reflux parameters when compared with PPI therapy. This improvement justifies the very high post-surgical symptom remission rate that we observed. Prolonged follow-up is warranted but our findings strongly support the surgical option in PPI failures.

The activity of pomegranate extract standardized 40% ellagic acid during the healing process of incision wounds in albino rats (Rattus norvegicus

Directory of Open Access Journals (Sweden)

Wiwik Misaco Yuniarti

2018-03-01

Full Text Available Aim: This research aimed to evaluate the effects of pomegranate extract standardized to 40% ellagic acid on the incised wound in albino rats. Materials and Methods: Fifty albino rats were divided into 10 treatment groups. The five groups were sacrificed on the 8th day, while the others were sacrificed on the 15th day. Two groups of albino rats with incised wound were not treated at all (P0, the other two groups of albino rats with incised wound were treated with Betadine® (P1 ointment, and the rest of the groups were treated with pomegranate extract standardized to 40% ellagic acid with a concentration of 2.5% (P2, 5% (P3, and 7.5% (P4. The treatments were carried out twice a day with an interval of 12 h for 7 and 14 days. At the end of the research, the skin tissue of those albino rats had been taken for histopathologic preparations before H and E staining was performed. Results: Collagen deposition, polymorphonuclear neutrophils (PMN infiltration, angiogenesis, and fibrosis degree in Group P4 treated with 7.5% pomegranate extract standardized to 40% ellagic acid for 14 days were significantly different from those in Groups P0, P1, P2, and P3, especially in the case of PMN inflammation (p<0.05. Conclusion: The administration of 7.5% pomegranate extract standardized to 40% ellagic acid for 14 days on incised wounds of those albino rats can accelerate the wound healing process characterized by collagen deposition improvement, PMN infiltration in the wound area, angiogenesis, and fibrosis degree.

Biological effects of brachytherapy using a {sup 32}P-patch on the skin of Sencar mice

Energy Technology Data Exchange (ETDEWEB)

Salgueiro, M.J. [Radioisotope Laboratory, School of Pharmacy and Biochemistry, University of Buenos Aires, Junin 956 Piso Bajo, 1113-Buenos Aires (Argentina)], E-mail: jsalgueiro@ffyb.uba.ar; Collia, N. [Radioisotope Laboratory, School of Pharmacy and Biochemistry, University of Buenos Aires, Junin 956 Piso Bajo, 1113-Buenos Aires (Argentina); Duran, H. [CONICET, San Martin (Argentina); School of Science and Technology, University of San Martin, San Martin (Argentina); Radiobiology Department, National Atomic Energy Commission (CNEA), Buenos Aires (Argentina); Palmieri, M. [CONICET, San Martin (Argentina); Biodiversity and Experimental Biology Department, School of Exact and Natural Sciences, University of Buenos Aires, Buenos Aires (Argentina); School of Science and Technology, University of San Martin, San Martin (Argentina); Medina, V. [Radioisotope Laboratory, School of Pharmacy and Biochemistry, University of Buenos Aires, Junin 956 Piso Bajo, 1113-Buenos Aires (Argentina); Ughetti, R.; Nicolini, J. [Research and Development Department, Laboratorios Bacon SAIC, Buenos Aires (Argentina); Zubillaga, M. [Radioisotope Laboratory, School of Pharmacy and Biochemistry, University of Buenos Aires, Junin 956 Piso Bajo, 1113-Buenos Aires (Argentina)

2009-10-15

In recent years, specially designed patches containing beta emitters have been developed for contact brachytherapy of skin lesions. The aim of the present work was to evaluate the biological effects of the {sup 32}P-patch on the skin of Sencar mice as a result of a brachytherapy treatment. For this purpose, a {sup 32}P-patch was prepared with Chromic {sup 32}P-phosphate and silicone and the classical model of two-stage skin carcinogenesis was reproduced in Sencar mice. Animals were divided in six groups. Four groups received the contact brachytherapy treatments using a scheme of a single session of 40 and 60 Gy (SD40 and SD60) and a scheme of two sessions of 40 and 60 Gy each (FD40 and FD60). The other two groups were used as controls of the single (CSD) and the fractionated (CFD) treatments. Radiation doses were estimated with equations derived from the MIRD DOSE scheme, and biologically effective doses (BED) were calculated according to equations derived from the linear-quadratic model. The endpoint to evaluate the treatments effects was tumor size after a follow-up period of 44 days. Finally, animals were sacrificed in order to get samples of all tumors for histological analysis and PCNA staining. Erythema, dermatitis and skin ulceration developed in almost all treated animals, but they gradually healed with regeneration of tissue during the follow-up period. Radiation effects on the skin of SD40, SD60, FD40 and FD60 showed a significant reduction of the tumor size with regard to controls, independently of the scheme and the radiation dose considered. PCNA staining scores of control groups were higher than for treated groups, independently of the scheme and the radiation dose considered. This radioactive {sup 32}P-silicone-patch which is easy to prepare and use in the treatment of skin diseases, seems promising as a radioactive device for clinical use.

Calcium phosphate coatings modified with zinc- or copper- incorporation on Ti-40Nb alloy

Science.gov (United States)

Komarova, E. G.; Sedelnikova, M. B.; Sharkeev, Yu P.; Kazakbaeva, A. A.; Glukhov, I. A.; Khimich, M. A.

2017-05-01

The influence of the microarc oxidation parameters and electrolyte composition on the structure, properties and composition of CaP coatings modified with Zn- or Cu- incorporation on the Ti-40mas.%Nb (Ti-40Nb) alloy was investigated. The linear growth of thickness, roughness, and size of structural elements with process voltage increasing has been revealed. It was shown that the CaP coatings have the low contact angles with liquids and, consequently, high free surface energy. This indicates a high hydrophilicity of the coatings. X-ray diffraction analysis showed that the coatings have X-ray amorphous structure. The increase of the process voltage leads to the formation of such crystalline phases as CaHPO4 and β-Ca2P2O7 in the coatings. The maximum Ca/P atomic ratio was equal to 0.4, and Zn or Cu contents was equal to 0.3 or 0.2 at.%, respectively.

Lethal modifications of DNA via the transmutation of 32P and 33P incorporated in the genome of the S13 bacteriophage

International Nuclear Information System (INIS)

Cols, P.; Apelgot, S.; Guille, E.

1988-01-01

When circular single-standard DNA of phage S13 is labelled with 32 P or 33 P, the transmutations very efficiently bring about a loss of phage infectiousness (efficiency = 1 for 32 P and 0.73 for 33 P). For both radionuclides, the lethal efficiencies as well as the lethal events are different. In the case of 32 P, the lethal event is the loss of the circular integrity of the DNA molecule, occurring as a consequence of a systematic single strand-break caused by each 32 P decay (100%). Conversely, in the case of 33 P, the lethal events are either a single strand-break (40%) or a local stereochemical modification (33%). The same primary event, the substitution at each 33 P decay of a phosphate by a sulfate molecule, leads to one of these lethal events in relation to the decay site. Moreover, neither the phage adsorption nor its genome injection into bacteria depends on the physical state of the genome, and thus lethality is revealed at only the genetic level. (orig.)

Hiina põlevkivikeemikud uurisid VKG tootmist / Peeter Lilleväli

Index Scriptorium Estoniae

Lilleväli, Peeter

2004-01-01

Ilmunud ka: Severnoje Poberezhje, 26. aug. 2004, lk. 1. Fushun Mining Group Co. Ltd juht Yin Liangi sõnul on nende firma tegelenud põlevkivist õli tootmisega üle 40 aasta, kuid kuna Eesti kogemus on sellel alal kaks korda pikem, huvituvad nad siin kasutatavast tehnoloogiast

Prognostic value of PINP, bone alkaline phosphatase, CTX-I, and YKL-40 in patients with metastatic prostate carcinoma

DEFF Research Database (Denmark)

Brasso, Klaus; Christensen, Ib Jarle; Johansen, Julia S.

2006-01-01

%, BAP (55%), CTX-I (33%), and YKL-40 (43%). Univariate analysis showed an association to survival: PINP (HR = 1.6, P 4, P 4, P = 0.004). In multivariate Cox analysis performance status, WHO grade, Soloway score, PINP...

Effect of low pH on the survival and emergence of aquatic insects

Energy Technology Data Exchange (ETDEWEB)

Bell, H L

1971-01-01

Mature larvae and nymphs of 9 species of aquatic insects (dragonflies, stoneflies, caddisflies, and mayfly) were tested in the laboratory at pH values from 1.0 to 7.0. The tl/sub 50/ values (pH at which 50 per cent of the organisms died) at 30 days ranged from pH 2.45 (Brachycentrus americanus) to pH 5.38 (Ephemeralla subvaria). The range at which 50 per cent of the insects emerged was pH 4.0 -5.9. The 9 species tested were all more sensitive to low pH during the period of emergence.

Combinatorial Dansyl Library and its Applications to pH-Responsive Probes.

Science.gov (United States)

Hong, Seong Cheol; Murale, Dhiraj P; Lee, Jun-Seok

2016-01-01

Herein, we report the first 48-membered, dansyl-based, combinatorial fluorescent library. From the electronic and structural properties of the probes, we analyzed their optical properties and chemical yields, with an average of 49 %. The molecules were examined for their pH responses, and DS-2 and DS-45 showed blue-shifts, whereas DS-7 and DS-40 showed red-shifts in wavelength with increasing pH. Finally, cell permeability was investigated by treating SNU-2292 cells. Our results demonstrate the potential application of this library in biosensors, bio-imaging and pH indicators.

The YKL-40 protein is a potential biomarker for COPD: a meta-analysis and systematic review

Directory of Open Access Journals (Sweden)

Tong X

2018-01-01

Full Text Available Xiang Tong,1 Dongguang Wang,1 Sitong Liu,1 Yao Ma,1,2 Zhenzhen Li,3 Panwen Tian,1,4 Hong Fan1 1Department of Respiratory and Critical Care Medicine, West China Hospital/West China School of Medicine, Sichuan University, Chengdu, People’s Republic of China; 2The Center of Gerontology and Geriatrics, West China Hospital/West China School of Medicine, Sichuan University, Chengdu, People’s Republic of China; 3Health Management Center, West China Hospital/West China School of Medicine, Sichuan University, Chengdu, People’s Republic of China; 4Lung Cancer Center, West China Hospital/West China School of Medicine, Sichuan University, Chengdu, People’s Republic of China Background: Many studies have found that YKL-40 may play an important pathogenic role in COPD. However, the results of these studies were inconsistent. Therefore, we performed a systematic review and meta-analysis to investigate the role of YKL-40 in COPD. Methods: We performed a systematic literature search in many database and commercial internet search engines to identify studies involving the role of YKL-40 in patients with COPD. The standardized mean difference (SMD and Fisher’s Z-value with its 95% confidence interval (CI were used to investigate the effect sizes. Results: A total of 15 eligible articles including 16 case–control/cohort groups were included in the meta-analysis. The results indicated that the serum YKL-40 levels in patients with COPD were significantly higher than those in healthy controls (SMD =1.58, 95% CI =0.68–2.49, P=0.001, and it was correlated with lung function (pooled r=-0.32; Z=-0.33; P<0.001. The results of subgroup analysis found that the serum YKL-40 levels were statistically different between the exacerbation group and the stable group in patients with COPD (SMD =1.55, 95% CI =0.81–2.30, P<0.001. Moreover, the results indicated that the sputum YKL-40 levels in patients with COPD were also significantly higher than those in healthy

Cadmium triggers Elodea canadensis to change the surrounding water pH and thereby Cd uptake.

Science.gov (United States)

Javed, M Tariq; Greger, Maria

2011-01-01

This study was aimed to investigate the influence of Elodea canadensis shoots on surrounding water pH in the presence of cadmium and the effect of plant-induced pH on cadmium uptake. The pH change in the surrounding nutrient solution and Cd uptake by Elodea shoots were investigated after cultivation of various plant densities (1, 3, 6 plants per 500 ml) in hydroponics at a starting pH of 4.0 and in the presence of different concentrations of cadmium (0, 0.1, 0.5 microM). Cadmium uptake was also investigated at different constant pH (4.0, 4.5, 5.5 and 6.5). To investigate if the pH change arose from photosynthetic activities, plants were grown under light, darkness or in the presence of a photosynthetic inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), and 0.5 microM cadmium in the solution. Elodea had an ability to increase the surrounding water pH, when the initial pH was low, which resulted in increased accumulation of Cd. The higher the plant density, the more pronounced was the pH change. The pH increase was not due to the photosynthetic activity since the pH rise was more pronounced under darkness and in the presence of DCMU. The pH increase by Elodea was triggered by cadmium.

All-Optical Regenerative OTDM Add-Drop Multiplexing at 40 Gb/s using Monolithic InP Mach-Zehnder Interferometer

DEFF Research Database (Denmark)

Fischer, St.; Dülk, M.; Gamper, E.

2000-01-01

We present a novel method for all-optical add-drop multiplexing having regenerative capability for 40-Gb/s optical time-division multiplexed (OTDM) data using a semiconductor optical amplifier (SOA) based, monolithic Mach-Zehnder interferometer (MZI). Simultaneous dropping of one 10-Gb/s channel ...

Effects of sulfite and pH an abscisic acid (ABA) dependent transpiration and on stomatal opening

Energy Technology Data Exchange (ETDEWEB)

Kondo, N.; Maruta, I.; Sugahara, K.

1980-01-01

In rice, alday, wheat and tobacco (Nicotiana tabacum l. samsun and samsun nn) plants which contained large amounts of ABA, the transpiration rate decreased rapidly with 2 ppM SO/sub 2/ fumigation and reached 20 to 65% of the initial level after 5- to 30-min exposure depending on their ABAj contents. In the cases of broad bean and tobacco (n. Gutinosa l.) with low ABA contents, the rate slightly increased for 20 and 40 min, respectively, after the start of the fumigation and then decreased gradually. The transpiration rates of corn and sorghum, in spite of their extremely low ABA contents, pronouncedly decreased with SO/sub 2/ fumigation and reached 65 and 50%, respectively, of the initial levels after 40-min exposure. Foliar application of 0.04 N HCL to N. tacum l. samsun nn leaves remarkably depressed the transpiration rate, while the application of 0.04 m NA/sub 2/SO/sub 3/ decreased the rate only to the same level as water treatment. Foliar application of either HCL of Na/sub 2/SO/sub 3/ to N. glutinosa l. leaves exerted little change in the transpiration rate. When 10-4 m ABA was applied to broad bean leaves prior to HCl and Na/sub 2/SO/sub 3/ treatment, their transpiration rate was decreased by HCl, but not by Na/sub 2/SO/sub 3/ application. In sonicated epidermal strips peeled from broad bean leaves, Na/sub 2/SO/sub 3/ produced a slight increase in the stomatal aperture size in the absence of ABA, but showed no effect in the presence of ABA. The aperture size was identical in the pH range of 3.0 to 7.0 in the incubation medium. In the presence of ABA in the medium, the aperture size was small in the acidic region of pH with a minimal value at pH 4.0. ABA decreased the aperture size at concentrations above 10-9 m at pH 4.0 and 10-6 m at pH 7.0 in the medium. ABA uptake by epidermal strips was large in the acidic region, especially at pH 4.0.

40 CFR 721.2098 - Aliphatic polycarboxylic acid metal salt (generic).

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Aliphatic polycarboxylic acid metal... Specific Chemical Substances § 721.2098 Aliphatic polycarboxylic acid metal salt (generic). (a) Chemical... as an aliphatic polycarboxylic acid metal salt (PMN P-01-7) is subject to reporting under this...

40 CFR 721.10089 - Modified salicylic acid, zirconium complex (generic).

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Modified salicylic acid, zirconium... Specific Chemical Substances § 721.10089 Modified salicylic acid, zirconium complex (generic). (a) Chemical... as modified salicylic acid, zirconium complex (PMN P-00-552) is subject to reporting under this...

40 CFR 721.720 - Alkoxylated fatty acid amide, alkylsulfate salt.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Alkoxylated fatty acid amide... Specific Chemical Substances § 721.720 Alkoxylated fatty acid amide, alkylsulfate salt. (a) Chemical... as an alkoxylated fatty acid amide, alkylsulfate salt (PMN P-97-136) is subject to reporting under...

Highly luminescent InP/GaP/ZnS QDs emitting in the entire color range via a heating up process.

Science.gov (United States)

Park, Joong Pill; Lee, Jae-Joon; Kim, Sang-Wook

2016-07-20

InP-based quantum dots (QDs) have attracted much attention for use in optical applications, and several types of QDs such as InP/ZnS, InP/ZnSeS, and InP/GaP/ZnS have been developed. However, early synthetic methods that involved rapid injection at high temperatures have not been able to reproducibly produce the required optical properties. They were also not able to support commercialization efforts successfully. Herein, we introduce a simple synthetic method for InP/GaP/ZnS core/shell/shell QDs via a heating process. The reaction was completed within 0.5 h and a full color range from blue to red was achieved. For emitting blue color, t-DDT was applied to prevent particle growth. From green to orange, color variation was achieved by adjusting the quantity of myristic acid. Utilizing large quantities of gallium chloride led to red color. With this method, we produced high-quality InP/GaP/ZnS QDs (blue QY: ~40%, FWHM: 50 nm; green QY: ~85%, FWHM: 41 nm; red QY: ~60%, FWHM: 65 nm). We utilized t-DDT as a new sulfur source. Compared with n-DDT, t-DDT was more reactive, which allowed for the formation of a thicker shell.

Highly luminescent InP/GaP/ZnS QDs emitting in the entire color range via a heating up process

Science.gov (United States)

Park, Joong Pill; Lee, Jae-Joon; Kim, Sang-Wook

2016-07-01

InP-based quantum dots (QDs) have attracted much attention for use in optical applications, and several types of QDs such as InP/ZnS, InP/ZnSeS, and InP/GaP/ZnS have been developed. However, early synthetic methods that involved rapid injection at high temperatures have not been able to reproducibly produce the required optical properties. They were also not able to support commercialization efforts successfully. Herein, we introduce a simple synthetic method for InP/GaP/ZnS core/shell/shell QDs via a heating process. The reaction was completed within 0.5 h and a full color range from blue to red was achieved. For emitting blue color, t-DDT was applied to prevent particle growth. From green to orange, color variation was achieved by adjusting the quantity of myristic acid. Utilizing large quantities of gallium chloride led to red color. With this method, we produced high-quality InP/GaP/ZnS QDs (blue QY: ~40%, FWHM: 50 nm green QY: ~85%, FWHM: 41 nm red QY: ~60%, FWHM: 65 nm). We utilized t-DDT as a new sulfur source. Compared with n-DDT, t-DDT was more reactive, which allowed for the formation of a thicker shell.

40 CFR 721.10097 - Disubstituted benzenesulfonic acid, alkali metal salt (generic).

Science.gov (United States)

2010-07-01

... chemical substance identified generically as disubstituted benzenesulfonic acid, alkali metal salt (PMN P... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Disubstituted benzenesulfonic acid, alkali metal salt (generic). 721.10097 Section 721.10097 Protection of Environment ENVIRONMENTAL...

Expression of Caspase-3, P53 in EL-4 cells induced by ionizing radiation and its biological implications

International Nuclear Information System (INIS)

Ju Guizhi; Shen Bo; Sun Shilong; Yan Fengqin; Fu Shibo; Li Pengwu

2006-01-01

Objective: To investigate the effect of ionizing radiation on the expressions of Caspase-3 and P53 proteins in EL-4 cells and its implications in the induction of apoptosis and polyploid cells. Methods: EL- 4 cells were irradiated with 4.0 Gy X-rays (180 kV, 15 mA, 0.287 Gy/min). Fluorescent staining and flow cytometry analysis were used to measure protein expression, apoptosis and polyploid cells. Results: It was found that the expression of Caspase-3 protein was increased significantly at 8 h and 12 h after the irradiation compared with sham-irradiated control (P<0.05), and the expression of P53 protein was also increased significantly at 2,4,8,12 and 24 h after the irradiation compared with sham-irradiated control (P<0.05 or P<0.01). The results showed that apoptosis of EL-4 cells was increased significantly at 2,4,8,12,24,48, and 72 h after 4.0 Gy irradiation compared with sham-irradiated control (P<0.05 or P<0.01 or P<0.001). However, no significant change in the number of polyploidy cells was found during the period from 2 to 48 h after the irradiation with 4.0 Gy X-rays. Conclusions: It is indicated that the expressions of Caspase-3 and P53 protein in EL-4 cells can be induced by ionizing radiation, and play an important role in the induction of apoptosis; the molecular pathway for polyploid formation might be P53-independent. (authors)

Elevated serum level of YKL-40 is an independent prognostic factor for poor survival in patients with metastatic melanoma

DEFF Research Database (Denmark)

Schmidt, Henrik; Johansen, Julia Sidenius; Gehl, Julie

2006-01-01

subjects. RESULTS:       Patients had higher serum YKL-40 values than healthy subjects (P poor performance status (P       = 0.002). Multivariate Cox analysis showed......BACKGROUND: YKL-40 is a growth factor for connective tissue cells and       stimulates migration of endothelial cells. Cancer cells, macrophages, and       neutrophils secrete YKL-40. Its function in cancer is unknown. High serum       YKL-40 levels have been associated with a poor prognosis...... was an independent prognostic factor for poor       survival in patients with metastatic melanoma. When combining serum YKL-40       and LDH, patients could be separated into three prognostic groups based on       the number of elevated biomarkers. The findings should be validated in an       independent study...

40 CFR 230.40 - Sanctuaries and refuges.

Science.gov (United States)

2010-07-01

... Section 230.40 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) OCEAN DUMPING SECTION... Impacts on Special Aquatic Sites § 230.40 Sanctuaries and refuges. (a) Sanctuaries and refuges consist of...; (2) Create unplanned, easy and incompatible human access to remote aquatic areas; (3) Create the need...

Relative Humidity of 40% Inhibiting the Increase of Pulse Rate, Body Temperature, and Blood Lactic Acid During Exercise

Directory of Open Access Journals (Sweden)

Nengah Sandi

2016-05-01

Full Text Available Background: Excessive sweating of the body is a reaction to decrease the heat caused by prolonged exercise at high relative humidity (RH. This situation may cause an increase in pulse rate (PR, body temperature (BT, and blood lactic acid (BLA workout. Objective: This study aimed to prove that a RH of 40% better than a RH of 50% and 60% RH in inhibiting the increase of PR, BT, and BLA during exercise. Methods: The study was conducted on 54 samples randomly selected from the IKIP PGRI Bali students. The samples were divided into three groups, and each group was given cycling exercise with a load of 80 Watt for 2 x 30 minutes with rest between sets for five minutes. Group-1 of cycling at 40% of RH, Group-2 at a RH of 50%, and the Group-3 at a RH of 60%. Data PR, BT, and BLA taken before and during exercise. The mean difference between groups before and during exercise were analyzed by One-way Anova and a further test used Least Significant Difference (LSD. Significance used was α = 0.05. Results: The mean of PR during exercise was significantly different between groups with p = 0.045, the mean of BT during exercises was significantly different between groups with p = 0.006, and the mean of BLA during exercises was significantly different between groups with p = 0.005 (p <0.05. Also found that PR, BT, and BLA during exercise at 40% RH was lower than 50% RH and 60% RH (p <0.05. Conclusion: Thus, the RH of 40% was better than RH of 50% and 60 % in inhibiting the increase of PR, BT, and BLA during exercise. Therefore, when practiced in a closed room is expected at 40% relative humidity.

Uptake of {sup 40}K and {sup 137}Cs in native plants of the Marshall Islands

Energy Technology Data Exchange (ETDEWEB)

Simon, S.L.; Graham, J.C.; Terp, S.D

2002-07-01

Uptake of {sup 137}Cs and {sup 40}K was studied in seven native plant species of the Marshall Islands. Plant and soil samples were obtained across a broad range of soil {sup 137}Cs concentrations (0.08-3900 Bq/kg) and a narrower range of {sup 40}K soil concentrations (2.3-55 Bq/kg), but with no systematic variation of {sup 40}K relative to {sup 137}Cs. Potassium-40 concentrations in plants varied little within the range of {sup 40}K soil concentrations observed. Unlike the case for {sup 40}K, {sup 137}Cs concentrations increased in plants with increasing {sup 137}Cs soil concentrations though not precisely in a proportionate manner. The best-fit relationship between soil and plant concentrations was P=aS{sup b} where a and b are regression coefficients and P and S are plant and soil concentrations, respectively. The exponent b for {sup 40}K was zero, implying plant concentrations were a single value, while b for {sup 137}Cs varied between 0.51 and 0.82, depending on the species. For both {sup 40}K and {sup 137}Cs, we observed a decreasing concentration ratio (where concentration ratio=plant concentration/soil concentration) with increasing soil concentrations. For the CR values, the best-fit relationship was of the form CR=aS{sup b}/S=aS{sup b-1}. For the {sup 40}K CR functions, the exponent b-1 was close to -1 for all species. For the {sup 137}Cs CR functions, the exponent b-1 varied from -0.19 to -0.48. The findings presented here, as well as those by other investigators, collectively argue against the usefulness of simplistic ratio models to accurately predict uptake of either {sup 40}K or {sup 137}Cs in plants over wide ranges of soil concentration.

A GPU-Based Genetic Algorithm for the P-Median Problem

OpenAIRE

AlBdaiwi, Bader F.; AboElFotoh, Hosam M. F.

2016-01-01

The p-median problem is a well-known NP-hard problem. Many heuristics have been proposed in the literature for this problem. In this paper, we exploit a GPGPU parallel computing platform to present a new genetic algorithm implemented in Cuda and based on a Pseudo Boolean formulation of the p-median problem. We have tested the effectiveness of our algorithm using a Tesla K40 (2880 Cuda cores) on 290 different benchmark instances obtained from OR-Library, discrete location problems benchmark li...

40 CFR 63.606 - Performance tests and compliance provisions.

Science.gov (United States)

2010-07-01

..., No. 3 Phosphorus-P2O5 or Ca3(PO4)2, Method B-Gravimetric Quimociac Method. (D) Section IX, Methods of..., Method B-Gravimetric Quimociac Method. (G) Section XI, Methods of Analysis For Phosphoric Acid... operator of an affected source shall use as reference methods and procedures the test methods in 40 CFR...

Characterisation and classification of RISOe P2546 cup anemometer

Energy Technology Data Exchange (ETDEWEB)

Friis Pedersen, T.

2004-03-01

The characteristics of the RISOe P2546 cup anemometer were investigated in detail, and all data presented in figures and tables. The characteristics include: wind tunnel calibrations, including an accredited calibration; tilt response meas-urements for tilt angles from -40 deg. C to 40 deg. C; gust response measurements at 8m/s, 10,5m/s and 13m/s and turbulence intensities of 10%, 16% and 23%; step response measurements at step wind speeds 4, 8, 12 and 15m/s; measurement of torque characteristics at 8m/s; rotor inertia measurements and measurements of friction of bearings at temperatures -20 deg. C to 40 deg. C. The characteristics are fitted to a time domain cup anemometer model, and the cup anemometer is put into the CLASSCUP classification scheme. The characteristics are also compared to the requirements to cup anemometers in the Danish wind turbine certification system and the CD and CDV of the revision of the standard IEC 61400-12. (au)

40 CFR 27.40 - Stay ordered by the Department of Justice.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Stay ordered by the Department of Justice. 27.40 Section 27.40 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL PROGRAM FRAUD CIVIL REMEDIES § 27.40 Stay ordered by the Department of Justice. If at any time the Attorney...

2,3,7,8-Tetrachlorodibenzo-p-dioxin-mediated disruption of the CD40 ligand-induced activation of primary human B cells

International Nuclear Information System (INIS)

Lu Haitian; Crawford, Robert B.; Kaplan, Barbara L.F.; Kaminski, Norbert E.

2011-01-01

Suppression of the primary antibody response is particularly sensitive to suppression by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in mice; however, surprisingly little is known concerning the effects of TCDD on humoral immunity or B cell function in humans. Results from a limited number of previous studies, primarily employing in vitro activation models, suggested that human B cell effector function is suppressed by TCDD. The present study sought to extend these findings by investigating, in primary human B cells, the effects of TCDD on several critical stages leading to antibody secretion including activation and plasmacytic differentiation using an in vitro CD40 ligand activation model. These studies revealed important differences in the response of human and mouse B cells to TCDD, the most striking being altered expression of plasmacytic differentiation regulators, B lymphocyte-induced maturation protein 1 and paired box protein 5, in mouse but not human B cells. The activation of human B cells was profoundly impaired by TCDD, as evidenced by decreased expression of activation markers CD80, CD86, and CD69. The impaired activation correlated with decreased cell viability, which prevented the progression of human B cells toward plasmacytic differentiation. TCDD treatment also attenuated the early activation of mitogen-activated protein kinases (MAPK) and Akt signaling in human B cells. Collectively, the present study provided experimental evidence for novel mechanisms by which TCDD impairs the effector function of primary human B cells. - Highlights: → In this study primary human and mouse B cell toxicity to TCDD was compared. → TCDD altered the expression of Blimp-1 and Pax5 in mouse but not human B cells. → TCDD markedly suppressed human B cell activation as characterized by CD80, CD86 and CD69 expression. → TCDD inhibited ERK, p38, and Akt phosphorylation in human B cells.

Identification of candidates for postmastectomy radiotherapy in patients with pT3N0M0 breast cancer

International Nuclear Information System (INIS)

Hamamoto, Yasushi; Ohsumi, Shozo; Aogi, Kenjiro; Takashima, Shigemitsu; Shinohara, Shuichi; Nakajima, Naomi; Kataoka, Masaaki

2013-01-01

There is still controversy concerning the indication of postmastectomy radiotherapy (PMRT) for pT3N0M0 breast cancer. To identify the candidates for PMRT in this subset, we investigated failure patterns, and searched for risk factors for isolated locoregional failure in pT3N0M0 breast cancer after mastectomy without PMRT. Among 1,176 patients who received mastectomy without PMRT for untreated unilateral breast cancer between 1990 and 2002, 64 patients (5%) had pT3N0M0 breast cancer (age 30-81 years; median 52.5 years). Isolated locoregional failure as the initial failure occurred in three patients. For all 64 patients, the 8-year failure-free survival rate, the isolated locoregional failure-free rate, and the distant failure-free rate were 76, 93, and 82%, respectively. Incidence of isolated locoregional failure as the initial failure was 18% (2/11) for patients 40 years or younger and 2% (1/53) for patients older than 40 years. The 8-year isolated locoregional failure-free rates were 73% for patients 40 years or younger and 98% for patients older than 40 years (p=0.0135). Concerning pT3N0M0 breast cancer, incidence of isolated locoregional failure was comparatively low after mastectomy without PMRT. Routine use of PMRT for all pT3N0M0 patients seemed to be unacceptable. PMRT may be useful for younger patients because of the comparatively high incidence of isolated locoregional failure. Because of the small number of cases in our series, further studies are necessary to determine the usefulness of PMRT for younger patients with pT3N0M0 breast cancer. (author)

40 CFR 467.40 - Applicability; description of the forging subcategory.

Science.gov (United States)

2010-07-01

... forging subcategory. 467.40 Section 467.40 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS ALUMINUM FORMING POINT SOURCE CATEGORY Forging Subcategory § 467.40 Applicability; description of the forging subcategory. This subpart applies to discharges of...

Microstructure of the Ni–Fe–Cu–P melt-spun ribbons produced from the single-chamber and from the double-chamber crucibles

Energy Technology Data Exchange (ETDEWEB)

Ziewiec, Krzysztof, E-mail: kziewiec@up.krakow.pl [Institute of Technology, Faculty of Mathematics, Physics and Technical Science, Pedagogical University of Cracow, ul. Podchorążych 2, PL-30-084 Kraków (Poland); Błachowski, Artur; Ruebenbauer, Krzysztof [Mössbauer Spectroscopy Division, Institute of Physics, Pedagogical University, ul. Podchorążych 2, PL-30-084 Kraków (Poland); Ziewiec, Aneta [AGH University of Science and Technology, Faculty of Metals Engineering and Industrial Computer Science, Al. A. Mickiewicza 30, 30-059 Kraków (Poland); Prusik, Krystian [Faculty of Computer Science and Materials Science, University of Silesia, ul. Bankowa 12, PL-40-007 Katowice (Poland); Latuch, Jerzy [Warsaw University of Technology, Faculty of Materials Science and Engineering, ul. Wołoska 141, PL-02-507 Warszawa (Poland); Zięba, Marcin; Bryła, Krzysztof [Institute of Technology, Faculty of Mathematics, Physics and Technical Science, Pedagogical University of Cracow, ul. Podchorążych 2, PL-30-084 Kraków (Poland)

2014-12-05

Highlights: • A new method for production of metallic amorphous/amorphous composite is proposed. • The unique microstructure was obtained by rapid cooling of the two unmixed liquids. • The composite TCMS Ni–Fe–Cu–P amorphous alloy forms ductile fracture. - Abstract: The aim of the work was to investigate the influence of the processing on the final microstructure and properties of the melt-spun Ni–Fe–Cu–P, Ni–Fe–P and Ni–Cu–P alloys ejected in two ways. In the first case, the alloy was molten in a simple single-chamber crucible, then ejected as uniform liquid. In the second case the double-chamber crucible was used, and the flux composed of the two Ni–Fe–P and Ni–Cu–P liquids was cooled on a copper roller before forming a uniform mixture. The two component melt spinning (TCMS) was performed starting from the Ni{sub 40}Fe{sub 40}P{sub 20} and Ni{sub 70}Cu{sub 10}P{sub 20} alloys. Three of the alloys i.e. Ni{sub 55}Fe{sub 20}Cu{sub 4}P{sub 20}, Ni{sub 40}Fe{sub 40}P{sub 20} and Ni{sub 70}Cu{sub 10}P{sub 20} were melt-spun from the traditional single-chamber crucible. The methods applied in this study for microstructural investigations include scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD) and Mössbauer spectroscopy. Thermal stability of the melt-spun alloys was tested using differential scanning calorimetry (DSC). The results of the investigations are described and discussed in terms of the unique features of the TCMS amorphous microstructure. It is shown that this complex phase composition of the amorphous alloy favors formation of the ductile fracture and the multiple shear band formation.

The "3"2P applicator combined with propranolol in the treatment of large area skin capillary hemangiomain children

International Nuclear Information System (INIS)

Duan Yongqiang; Wu Zhenfu; Wu Min; Qiu Xuan; Fei Shinuan

2016-01-01

Objective: To investigate the clinical efficacy of "3"2P applicator plus propranolol in children with a large area of skin capillary hemangioma. Methods: Forty five cases of large hemangioma were divided into two groups. Control group of 20 patients recieved the conventional "3"2P application therapy. Of 20 cases in control group, there were 10 cases ≤3 years old children with hemangiomas, and 10 cases > 3 years old children with Port Wine Stain(PWS). Observation group of 25 cases recieved "3"2P applicator plus propranolol. Of 25 cases in observation group 10 cases werer ≤3 years old children with hemangiomas, 15 cases of children with PWS. Comparison was made between two different types of vascular tumors, treatment in ≤3 years old children and > 3 years old children, and adverse reactions. Data statistical analysis used χ"2 test and zero reaction test. P 0.05). The treatment efficacy in ≤3 years old children with infant hemangioma was 90.0% in control group and 100% in observation group (P > 0.05 ). The treatment efficacy for PWS in > 3 years old children was 40.0% in control group and 80% in observation group. Here the difference is statistically significant(P 3 years old children in control group,the treatment efficacy was 90.0% and 40.0%, respectively P 3 years old children in observation group, the treatment efficacy was 100.0% and 80.0% with no significant difference(P > 0.05). Complication of moist dermatitis was 25.0% in control group and 40.0% in observation group. Occurrence of depigmentation was 75.0% and 84.0% in control and observation group with no significant difference(P > 0.05). Changing in heart rate occurs in ≤3 years old children and > 3 years old children, 50.0% and 40.0%, respectively, with no significant difference(P > 0.05). Conclusion: "3"2P applicator plus propranolol treatment of refractory large hemangioma is simple, safe, and effective. But close attention needs to be paid to both "3"2P and propranolol on adverse reactions

Binding of the cyclic AMP receptor protein of Escherichia coli and DNA bending at the P4 promoter of pBR322.

Science.gov (United States)

Brierley, I; Hoggett, J G

1992-07-01

The binding of the Escherichia coli cyclic AMP receptor protein (CRP) to its specific site on the P4 promoter of pBR322 has been studied by gel electrophoresis. Binding to the P4 site was about 40-50-fold weaker than to the principal CRP site on the lactose promoter at both low (0.01 M) and high (0.1 M) ionic strengths. CRP-induced bending at the P4 site was investigated from the mobilities of CRP bound to circularly permuted P4 fragments. The estimated bending angle, based on comparison with Zinkel & Crothers [(1990) Biopolymers 29, 29-38] A-tract bending standards, was found to be approximately 96 degrees, similar to that found for binding to the lac site. These observations suggest that there is not a simple relationship between strength of CRP binding and the extent of induced bending for different CRP sites. The apparent centre of bending in P4 is displaced about 6-8 bp away from the conserved TGTGA sequence and the P4 transcription start site.

High-resolution measurements of sup(58,60,62)Ni(p,p) scattering cross section in the proton energy range of 3.0 to 4.0 MeV

International Nuclear Information System (INIS)

Arai, Eiichi; Ogawa, Masao

1976-01-01

Differential cross sections were measured at four angles for proton scattering on sup(58,60,62)Ni at energies from 3.0 to 4.0 MeV by using a high-resolution beam from the Tokyo Institute of Technology 4 MV Van de Graaff. An overall resolution of 400 eV (FWHM) was realized using thin solid targets. (author)

40 CFR 721.4663 - Fluorinated carboxylic acid alkali metal salts.

Science.gov (United States)

2010-07-01

... Specific Chemical Substances § 721.4663 Fluorinated carboxylic acid alkali metal salts. (a) Chemical... fluorinated carboxylic acid alkali metal salts (PMNs P-95-979/980/981) are subject to reporting under this... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Fluorinated carboxylic acid alkali...

COMPARATIVE ANALYSIS OF SOLUBLE OF CD40 LIGAND LEVELS IN HEART RECIPIENTS TREATED WITH CYCLOSPORINE A AND TACROLIMUS

Directory of Open Access Journals (Sweden)

O. P. Shevchenko

2012-01-01

Full Text Available Soluble form of CD40L is platelet activating factor, which is a marker of inflammation and thrombosis. Elevated levels of sCD40L before the heart transplantation are associated with the risk of early development of cardiova- scular complications. The study included 54 patients who had received heart transplants. All recipients received a triple heart immu- nosuppressive therapy, including methylprednisolone, mycophenolate mofetil and cyclosporine A (20 recipients or methylprednisolone, mycophenolate mofetil and tacrolimus (34 recipients. Patients were not differed by age, gender, etiology of heart failure before heart transplantation (p > 0,05. In the first group of transplant recipients, the relative risk of cardiovascular events with high sCD40L levels before transplantation was 3 2 (95% CI 1,4; 12,0. In the second group of recipients, respectively, 2.69 (95% CI 1,1; 8,5. SCD40L level after heart transplan- tation was significantly higher for patients receiving cyclosporine (P < 0.05. Increasing concentrations of sCD40L are associated with a higher incidence of cardiovascular complications. 

Characterization of a Broadly Reactive Anti-CD40 Agonistic Monoclonal Antibody for Potential Use as an Adjuvant.

Directory of Open Access Journals (Sweden)

Cameron Martin

Full Text Available Lack of safe and effective adjuvants is a major hindrance to the development of efficacious vaccines. Signaling via CD40 pathway leads to enhanced antigen processing and presentation, nitric oxide expression, pro-inflammatory cytokine expression by antigen presenting cells, and stimulation of B-cells to undergo somatic hypermutation, immunoglobulin class switching, and proliferation. Agonistic anti-CD40 antibodies have shown promising adjuvant qualities in human and mouse vaccine studies. An anti-CD40 monoclonal antibody (mAb, designated 2E4E4, was identified and shown to have strong agonistic effects on primary cells from multiple livestock species. The mAb recognize swine, bovine, caprine, and ovine CD40, and evoked 25-fold or greater proliferation of peripheral blood mononuclear cells (PBMCs from these species relative to cells incubated with an isotype control (p<0.001. In addition, the mAb induced significant nitric oxide (p<0.0001 release by bovine macrophages. Furthermore, the mAb upregulated the expression of MHC-II by PBMCs, and stimulated significant (p<0.0001 IL-1α, IL6, IL-8, and TNF-α expression by PBMCs. These results suggest that the mAb 2E4E4 can target and stimulate cells from multiple livestock species and thus, it is a potential candidate for adjuvant development. This is the first study to report an anti-swine CD40 agonistic mAb that is also broadly reactive against multiple species.

Formulation and comparative in vitro evaluation of various dexamethasone-loaded pH-sensitive polymeric nanoparticles intended for dermal applications.

Science.gov (United States)

Sahle, Fitsum Feleke; Gerecke, Christian; Kleuser, Burkhard; Bodmeier, Roland

2017-01-10

pH-sensitive nanoparticles have a great potential for dermal and transfollicular drug delivery. In this study, pH-sensitive, dexamethasone-loaded Eudragit ® L 100, Eudragit ® L 100-55, Eudragit ® S 100, HPMCP-50, HPMCP-55 and cellulose acetate phthalate nanoparticles were prepared by nanoprecipitation and characterized. The pH-dependent swelling, erosion, dissolution and drug release kinetics were investigated in vitro using dynamic light scattering and Franz diffusion cells, respectively. Their toxicity potential was assessed by the ROS and MTT assays. 100-700nm nanoparticles with high drug loading and entrapment efficiency were obtained. The nanoparticles bear no toxicity potential. Cellulose phthalates nanoparticles were more sensitive to pH than acrylates nanoparticles. They dissolved in 10mM pH 7.5 buffer and released>80% of the drug within 7h. The acrylate nanoparticles dissolved in 40mM pH 7.5 buffer and released 65-70% of the drug within 7h. The nanoparticles remained intact in 10 and 40mM pH 6.0 buffers (HPMCP nanoparticles dissolved in 40mM pH 6.0 buffer) and released slowly. The nanoparticles properties could be modulated by blending the different polymers. In conclusion, various pH-sensitive nanoparticles that could release differently on the skin surface and dissolve and release in the hair follicles were obtained. Copyright © 2016 Elsevier B.V. All rights reserved.

Evaluation of pH at the Bacteria–Dental Cement Interface

Science.gov (United States)

Mayanagi, G.; Igarashi, K.; Washio, J.; Nakajo, K.; Domon-Tawaraya, H.; Takahashi, N.

2011-01-01

Physiochemical assessment of the parasite-biomaterial interface is essential in the development of new biomaterials. The purpose of this study was to develop a method to evaluate pH at the bacteria-dental cement interface and to demonstrate physiochemical interaction at the interface. The experimental apparatus with a well (4.0 mm in diameter and 2.0 mm deep) was made of polymethyl methacrylate with dental cement or polymethyl methacrylate (control) at the bottom. Three representative dental cements (glass-ionomer, zinc phosphate, and zinc oxide-eugenol cements) were used. Each specimen was immersed in 2 mM potassium phosphate buffer for 10 min, 24 hrs, 1 wk, or 4 wks. The well was packed with Streptococcus mutans NCTC 10449, and a miniature pH electrode was placed at the interface between bacterial cells and dental cement. The pH was monitored after the addition of 1% glucose, and the fluoride contained in the cells was quantified. Glass-ionomer cement inhibited the bacteria-induced pH fall significantly compared with polymethyl methacrylate (control) at the interface (10 min, 5.16 ± 0.19 vs. 4.50 ± 0.07; 24 hrs, 5.20 ± 0.07 vs. 4.59 ± 0.11; 1 wk, 5.34 ± 0.14 vs. 4.57 ± 0.11; and 4 wks, 4.95 ± 0.27 vs. 4.40 ± 0.14), probably due to the fluoride released from the cement. This method could be useful for the assessment of pH at the parasite-biomaterial interface. PMID:21933936

Levels of 137Cs and 40K in wood ash-amended soils

International Nuclear Information System (INIS)

Ohno, Tsutomu; Hess, C.T.

1994-01-01

Wood ash is a residual material produced at an annual rate of 1.5-3.0 million tons by wood burning power plants in the USA. Up to 80% of the wood ash generated in northeastern USA is landspread on agricultural soils. Recently, concern has arisen regarding the 137 Cs content of wood ash and levels of 137 Cs of wood ash-amended soils. The 137 Cs originated primarily from above ground nuclear weapons testing in the 1950s and 1960s. This study examined the total and pH 3, NH 4 OAc extractable levels of 137 Cs and 40 K in three soils incubated in the laboratory with 0, 3 and 9 g of wood ash on a calcium carbonate equivalence basis kg -1 soil. The wood ash contained 137 Cs and 40 K at 3920 and 21'700 pCi kg -1 , respectively. At the regulated wood ash application rate limit, 3 g wood ash (calcium carbonate equivalent basis) kg -1 of soil, there was no statistical difference from the control treatment in both total and soluble 137 Cs and 40 K levels. For one soil, there was an increase in the 137 Cs level when wood ash was amended at 9 g wood ash (calcium carbonate equivalent basis) kg -1 soil. The 137 Cs was strongly bound to the cation exchange sites of the soils with the average fraction soluble in pH 3, NH 4 OAc solution at 4.8% in the mineral soils and 0.9% in the organic soil. Considering the current limits on permitted wood ash application rates to soils, there was no statistically significant effect on the levels of 137 Cs or 40 K found in wood ash-amended soils

Is bark pH more important than tree species in determining the composition of nitrophytic or acidophytic lichen floras?

Energy Technology Data Exchange (ETDEWEB)

Spier, L. [Alterra, Landscape Center, POB 47, 6700 AA Wageningen (Netherlands); Dobben, H. van, E-mail: han.vandobben@wur.n [Alterra, Landscape Center, POB 47, 6700 AA Wageningen (Netherlands); Dort, K. van [Alterra, Landscape Center, POB 47, 6700 AA Wageningen (Netherlands)

2010-12-15

To study the pH preference of epiphytic lichens, the bark pH of Fraxinus, Tilia, Quercus and Ulmus trees in an urban environment was measured using a flat surface electrode. The total number of trees was 253. A survey was made of the lichens in a 40 x 40 cm quadrat surrounding the pH measurement point. Our data analysis using multivariate and univariate statistical techniques indicates that the tree species is the most important factor influencing lichen colonisation, and that bark pH alone is of less importance. We hypothesize that the changed pollution climate, with strong decreases in both sulphur dioxide and ammonia concentrations over the past two decades and a concomitant general increase in bark pH, has made epiphytes less sensitive to pH. - Tree species, rather than bark pH determines the occurrence of acidophytes and nitrophytes on trees.

Structure of s - p bonded metal clusters with 8, 20 and 40 valence electrons

International Nuclear Information System (INIS)

Kumar, V.

1992-10-01

From studies on some clusters of metals and semiconductors, there appear some similarities in the structure of clusters with a given number of atoms and having the number of valence electrons corresponding to a shell closing. Here we present results of the atomic and electronic structure of a few other clusters with 20 and 40 valence electrons, namely Sb 4 , Sn 5 and Sb 8 using the density functional molecular dynamics method. We suggest that the similarities in the structure and deviation from them may help to understand bonding characteristics in clusters and its evolution to bulk behaviour. Our results on Sb 8 cluster are preliminary but indicate that above room temperature its structure is two weakly interacting tetrahedra which is in general agreement with the observation of predominently antimony tetramers at T > 300 K. (author). 16 refs, 2 figs

Evidence for the reaction πp → etaetan at 40 GeV/c

International Nuclear Information System (INIS)

Apel, W.D.; Augenstein, K.; Bertolucci, E.

1975-01-01

An experiment investigating the reaction π - +p → mγ + n has been performed at the 70 GeV IHEP-accelerator. The final state gammas are measured in a hodoscope spectrometer running on-line with a computer. In a preliminary study of data events of the reaction π - p → etaetan (eta → 2γ) are observed. At invariant masses of the etaeta-system above 1600 MeV up to 2800 MeV a clean sample free of background from π 0 π 0 -events is obtained. The ratio of production cross sections of etaeta and π 0 π 0 final states of the order 1:5 which has been found is in agreement with predictions of the quark model. Etaeta angular distributions in the Gottfried-Jackson frame for masses above 1600 MeV are not isotopic

Radiotherapy for tongue cancer in young adults less than 40 years

International Nuclear Information System (INIS)

Yoshida, Ken; Koizumi, Masahiko; Inoue, Toshihiko; Inoue, Takehiro; Yamazaki, Hideya; Kagawa, Kazufumi; Teshima, Teruki; Shimizutani, Kimishige; Furukawa, Souhei; Murayama, Shigeyuki; Nose, Takayuki; Tanaka, Eiichi

1996-01-01

Purpose/Objective: Radiotherapy for tongue cancer in young adults less than 40 years is studied, retrospectively. Some reports say young tongue cancer patient has worse prognosis. We investigate treatment results of them treated in our department, compared with elder patients. Materials and Methods: Our department registered 1289 patients with tongue cancer during 26 years between Jan. 1967 and Dec. 1992. One hundred and eighty-eight patients were under 40 years old. One hundred and forty-eight patients were primarily treated by radiotherapy. Follow-up terms are among 2 and 25 years (median; 15 years). They consisted of 99 males and 49 females. They were divided into 3 age groups, 3 teenagers, 39 twenties, and 106 thirties. The youngest was 18 years old. T and N categories were T1: 33, T2: 77, T3: 34, T4: 4, N0: 111, N1: 25, N2: 4, and N3: 8 patients, respectively. One hundred and twenty patients were treated only with radiotherapy. Twenty-four and 4 patients were treated with chemotherapy and surgery adding to radiotherapy respectively. Radiotherapy consisted of 29 external, 60 brachytherapy, and 59 both. One hundred and nineteen brachytherapies consisted of Ir: 66, Ra: 50, Ir+Ra: 1, and Au: 2. Eighty-one patients with T1 or2N0M0 treated only by radiotherapy were compared with the similar 454 patients more and equal to 40 years old. Results: The 5-and 10-year survival rates of 148 patients were 69% and 65%, respectively. The 5-and 10-year local control rates were 77% and 76%, respectively. The 5-year survival rates of T1, T2, T3, and T4 patients were 90%, 70%, 50%, and 43%, respectively. The corresponding 10-year survival rates were 81%, 66%, 50%, and 43%, respectively. The 5-year local control rates were 87%, 79%, 71%, and 25%, respectively. With respect to T1 or 2N0M0 patients, the 5-and 10-year local control rates were 80% and 75% under 40 years old, 77% and 73% over 40 years old patients. (p=0.99) The 5-and 10-year cause-specific survival rates were 79% and 72

Antiproton-proton elastic scattering at 3.0 and 4.0 GeV/C; Difusion elastica antiproton-proton a 3,0 y 4,0 GeV/C

Energy Technology Data Exchange (ETDEWEB)

Unamuno, S

1965-07-01

This paper presents the results-obtained in studying the two-prong interactions observed in the Saclay 81 cm hydrogen bubble chamber exposed to the 3.0 and 4.0 GeV/c antiproton beams from CERN Proton-Synchroton. Total elastic cross-sections corresponding to both energies are given. The results are given. The results are compared with those of p-p scaterring at different energies and with those of p-p scattering. Several optical-models, from the simples one (the black disk model) to a rather elaborated, four-parameters model have been applied. These models can explain some of the experimental results but fail in predicting the angular distribution of large angle scattering. (Author)

Elevated YKL40 is associated with advanced prostate cancer (PCa) and positively regulates invasion and migration of PCa cells.

Science.gov (United States)

Jeet, Varinder; Tevz, Gregor; Lehman, Melanie; Hollier, Brett; Nelson, Colleen

2014-10-01

Chitinase 3-like 1 (CHI3L1 or YKL40) is a secreted glycoprotein highly expressed in tumours from patients with advanced stage cancers, including prostate cancer (PCa). The exact function of YKL40 is poorly understood, but it has been shown to play an important role in promoting tumour angiogenesis and metastasis. The therapeutic value and biological function of YKL40 are unknown in PCa. The objective of this study was to examine the expression and function of YKL40 in PCa. Gene expression analysis demonstrated that YKL40 was highly expressed in metastatic PCa cells when compared with less invasive and normal prostate epithelial cell lines. In addition, the expression was primarily limited to androgen receptor-positive cell lines. Evaluation of YKL40 tissue expression in PCa patients showed a progressive increase in patients with aggressive disease when compared with those with less aggressive cancers and normal controls. Treatment of LNCaP and C4-2B cells with androgens increased YKL40 expression, whereas treatment with an anti-androgen agent decreased the gene expression of YKL40 in androgen-sensitive LNCaP cells. Furthermore, knockdown of YKL40 significantly decreased invasion and migration of PCa cells, whereas overexpression rendered them more invasive and migratory, which was commensurate with an enhancement in the anchorage-independent growth of cells. To our knowledge, this study characterises the role of YKL40 for the first time in PCa. Together, these results suggest that YKL40 plays an important role in PCa progression and thus inhibition of YKL40 may be a potential therapeutic strategy for the treatment of PCa. © 2014 The authors.

Influence of dietary calcium (Ca) and phosphorus (P) on phosphorus retention in chickens

International Nuclear Information System (INIS)

Scheideler, S.E.; Sell, J.L.

1986-01-01

Experiment 1 was a 3 x 3 factorial arrangement with 3 levels of dietary Ca (3.0, 3.5, and 4.0%) and 3 P feeding programs fed to laying hens for 336 days. The P programs tested were: .64% P fed continuously for 48 weeks; .64, .54, and .44% P; and .56, .49, and .39% P fed when hens were 24 to 36, 36 to 52, and 52 to 72 weeks of age, respectively. In addition to production traits, measurements of P retention were made at 34, 42, 50, 62, and 72 weeks of age. None of the production traits measured showed any dietary effects. Dietary Ca affected P retention at 50, 62, and 72 weeks of age. None of the production traits measured showed any dietary effects. Dietary Ca affected P retention at 50, 62, and 72 weeks of age. Average P retention for the experiment was 104, 148, and 178 mg/day in hens fed 3.0, 3.5, and 4.0% Ca. Dietary P affected P retention at 42 and 72 weeks, with P retention being greater in hens fed .64% P. Ca x P interactions were measured at 42, 62, and 72 weeks of age. Experiment 2 involved 2 levels of dietary Ca (.8 and 1.2%) fed to one-week-old broiler chicks for 8 days during which time 50 uCi 33 P/chick were administered. Serum, femurs, and excreta were collected 48 hours following 33 P dosing. Serum inorganic P and excreted 33 P activity were greater in chicks fed .8% Ca; whereas femur 33 P activity was greater when chicks received the 1.2% Ca diet. These data indicate increased P retention and greater bone deposition of P in chicks fed 1.2% dietary Ca. These results support those of Experiment 1 in which P retention of hens increased as dietary Ca increased

Internal magnetic probe data from ZT-40

International Nuclear Information System (INIS)

Burkhardt, L.C.; Phillips, J.A.

1981-05-01

Measurements of magnetic field distribution as a function of time and radius were made in ZT-40 with its ceramic vacuum vessel. Data were obtained with a 10-station, 20-coil magnetic probe, measuring the B/sub p/ and B/sub epsilon/ orthogonal field components in deuterium plasma discharges. Sheath formation and diffusion, magnetic axis location and motion, the effect of the probe on the plasma, and the consistency of flux measurements with external probes are examined

Limits on the anomalous ZZγ and Zγγ couplings in p bar p collisions at √s=1.8 TeV

International Nuclear Information System (INIS)

Abachi, S.; Abbott, B.; Abolins, M.; Acharya, B.S.; Adam, I.; Adams, D.L.; Adams, M.; Ahn, S.; Aihara, H.; Alitti, J.; Alvarez, G.; Alves, G.A.; Amidi, E.; Amos, N.; Anderson, E.W.; Aronson, S.H.; Astur, R.; Avery, R.E.; Baden, A.; Balamurali, V.; Balderston, J.; Baldin, B.; Bantly, J.; Bartlett, J.F.; Bazizi, K.; Bendich, J.; Beri, S.B.; Bertram, I.; Bezzubov, V.A.; Bhat, P.C.; Bhatnagar, V.; Bhattacharjee, M.; Bischoff, A.; Biswas, N.; Blazey, G.; Blessing, S.; Boehnlein, A.; Bojko, N.I.; Borcherding, F.; Borders, J.; Boswell, C.; Brandt, A.; Brock, R.; Bross, A.; Buchholz, D.; Burtovoi, V.S.; Butler, J.M.; Casey, D.; Castilla-Valdez, H.; Chakraborty, D.; Chang, S.; Chekulaev, S.V.; Chen, L.; Chen, W.; Chevalier, L.; Chopra, S.; Choudhary, B.C.; Christenson, J.H.; Chung, M.; Claes, D.; Clark, A.R.; Cobau, W.G.; Cochran, J.; Cooper, W.E.; Cretsinger, C.; Cullen-Vidal, D.; Cummings, M.; Cutts, D.; Dahl, O.I.; De, K.; Demarteau, M.; Demina, R.; Denisenko, K.; Denisenko, N.; Denisov, D.; Denisov, S.P.; Dharmaratna, W.; Diehl, H.T.; Diesburg, M.; Di Loreto, G.; Dixon, R.; Draper, P.; Drinkard, J.; Ducros, Y.; Dugad, S.R.; Durston-Johnson, S.; Edmunds, D.; Efimov, A.O.; Ellison, J.; Elvira, V.D.; Engelmann, R.; Eno, S.; Eppley, G.; Ermolov, P.; Eroshin, O.V.; Evdokimov, V.N.; Fahey, S.; Fahland, T.; Fatyga, M.; Fatyga, M.K.; Featherly, J.; Feher, S.; Fein, D.; Ferbel, T.; Finocchiaro, G.; Fisk, H.E.; Fisyak, Y.; Flattum, E.; Forden, G.E.; Fortner, M.; Frame, K.C.; Franzini, P.; Fredriksen, S.; Fuess, S.; Galjaev, A.N.; Gallas, E.; Gao, C.S.; Gao, S.; Geld, T.L.; Genik, R.J. II; Genser, K.; Gerber, C.E.; Gibbard, B.; Glebov, V.; Glenn, S.; Gobbi, B.; Goforth, M.; Goldschmidt, A.; Gomez, B.; Goncharov, P.I.; Gordon, H.; Goss, L.T.; Graf, N.; Grannis, P.D.; Green, D.R.; Green, J.; Greenlee, H.; Griffin, G.; Grossman, N.; Grudberg, P.; Gruenendahl, S.; Guida, J.A.; Guida, J.M.; Guryn, W.; Gurzhiev, S.N.; Gutnikov, Y.E.; Hadley, N.J.; Haggerty, H.; Hagopian, S.

1995-01-01

We performed a direct search for the anomalous ZZγ and Zγγ couplings by studying p bar p→llγ+X (l=e,μ) events at √s=1.8 TeV with the D0 detector at the Fermilab Tevatron Collider. A fit to the transverse energy spectrum of the photon in the signal events, based on the data set corresponding to an integrated luminosity of 14.3pb -1 (13.7pb -1 ) for the electron (muon) channel, yields the following 95% confidence level limits on the anomalous CP-conserving ZZγ couplings: |h 30 Z | 40 Z =0) and |h 40 Z | 30 Z =0), for a form-factor scale Λ=500 GeV. Limits for the Zγγ couplings and CP-violating couplings are also discussed

Search for associated production of charmed particles in π-p collisions at 40 GeV/c

International Nuclear Information System (INIS)

Apel', V.D.; Bertoluchchi, E.; Viktorov, V.A.

1976-01-01

At the 70 GeV IHEP accelerator a search for the associated production of charmed D mesons produced in the reaction π - p→D 0 anti D 0 n at 40 GeV/c incident pion momentum was performed. For D 0 →K 0 π 0 decay identification a system of multiwire spark and proportional chambers and hodoscopes were used, detecting Ksub(s)sup(0)→πsup(+)πsup(-) decay, and 648-channel hodoscope spectrometer with the on-line computer was used to detect γ's from π 0 decay

Altas concentrações de FSH-p na maturação in vitro de oócitos Bos indicus High concentrations of FSH-p on the in vitro maturation of Bos indicus oocytes

Directory of Open Access Journals (Sweden)

Joana D'Arc Rocha Alves

2001-08-01

Full Text Available O objetivo deste trabalho foi avaliar a eficiência de diferentes concentrações de um FSH-p comercial sobre a maturação nuclear de oócitos Bos indicus, clivagem e desenvolvimento in vitro de embriões até estádios de blastocisto. Após seleção e transferência para o meio TCM 199/HEPES suplementado com diferentes concentrações de FSH-p (T1 = 10mg/m ; T2 = 20mg/m ; T3 = 40mg/m, os oócitos foram incubados, durante 24 horas, a 39ºC em atmosfera úmida contendo 5% de CO2. Parte dos oócitos foram retirados para análise da maturação nuclear e os demais foram transferidos para o meio de fecundação (mDM. Após 18 horas de incubação nas mesmas condições atmosféricas mencionadas para os oócitos, os presumíveis zigotos foram distribuídos no meio de desenvolvimento embrionário (KSOM contendo monocamada de células da granulosa. As porcentagens de metáfase II, de clivagem e de blastocisto foram, respectivamente, de 81,8/62,5/17,6% (T1; 55,6/64,0/19,5% (T2 e 50,0/65,0/16,3% (T3. A análise estatística revelou que uma menor porcentagem (P £ 0,05 de oócitos tratados com 20mg/m e 40mg/m de FSH-p alcançou o estádio de metáfase II e que as taxas de clivagem e blastocisto não diferiram (P ³ 0,05 entre os tratamentos. Os resultados permitem concluir que a adição de 20mg/m e 40mg/m de FSH-p ao meio de cultura interfere no processo de maturação nuclear, mas todas as concentrações testadas podem ser utilizadas sem prejuízo aparente para a clivagem e o posterior desenvolvimento embrionário.The aim of this work was to evaluate the efficiency of different concentrations of a commercial FSH-p on the nuclear maturation of Bos indicus oocytes, cleavage and in vitro development of embryos until blastocyst stages. The oocytes were selected and transferred to the maturation medium (TCM 199/25 mM HEPES supplemented with different concentrations of FSH-p (T1 = 10mg/m ; T2 - 20mg/m ; T3 - 40mg/m and after 24 hours of incubation, at 39º

Ξ-P Scattering and STOPPED-Ξ-12C Reaction

Science.gov (United States)

Ahn, J. K.; Aoki, S.; Chung, K. S.; Chung, M. S.; En'yo, H.; Fukuda, T.; Funahashi, H.; Goto, Y.; Higashi, A.; Ieiri, M.; Iijima, T.; Iinuma, M.; Imai, K.; Itow, Y.; Lee, J. M.; Makino, S.; Masaike, A.; Matsuda, Y.; Matsuyama, Y.; Mihara, S.; Nagoshi, C.; Nomura, I.; Park, I. S.; Saito, N.; Sekimoto, M.; Shin, Y. M.; Sim, K. S.; Susukita, R.; Takashima, R.; Takeutchi, F.; Tlustý, P.; Weibe, S.; Yokkaichi, S.; Yoshida, K.; Yoshida, M.; Yoshida, T.; Yamashita, S.

2000-09-01

We report upper limits on the cross sections for the Ξ-p elastic and conversion processes based on the observation of one Ξ-p elastic scattering events with an invisible Λ decay. The cross section for the Ξ-p elastic scattering is, for simplicity, assumming an isotropic angular distribution, found to be 40 mb at 90% confidence level, whereas that for the Ξ-p → ΛΛ reaction is 11 mb at 90% confidence level. While the results on the elastic cross section give no stringent constraint on theoretical estimates, the upper limit on the conversion process suggests that the estimate of the RGM-F model prediction could be ruled out. We also report some preliminary results on the obervation of the stopped-Ξ- hyperon-nucleus interaction with respect to hypernuclear production and existence of doubly-strange H-dibaryon.

Geological Dating by 40 Ar - 39 Ar method

International Nuclear Information System (INIS)

Vollbert Romero, M.E.

1992-01-01

The isotope 40 K is radioactive, it decays to 40 Ar stable. The number of 40 Ar atoms produced from 40 K, permits to calculate the date of rocks and minerals. This dating technique is named 'Conventional K-Ar Dating Method'. The 40 Ar - 39 Ar dating method permits to calculate the age of rocks and minerals eliminating the limitation of the K-Ar method by calculating potassium and argon concentrations in a single measurement of the ratio of argon isotopes. In this work, the irradiation of the sample with fast neutrons in the nuclear reactor was established. 39 Ar is obtained from the induced reaction 39 K (n,p) 39 Ar. Thus the ration of 40 Ar - 39 Ar allows to obtain the date of rocks and minerals. This ratio was measured in a mass spectrometer. If the measurement of argon concentration in the sample is carried out at different increasing temperature values, it is possible to get information of paleotemperatures. The number of atoms 39 Ar is a function of the number 39 K atoms, irradiation time, neutrons flux, its energy E and the capture cross section σ of 39 K. These parameters are calculate indirectly by obtaining the so called 'J value ' by using a standard mineral with known age (HD-BI y Biot-133), this mineral is irradiated together with the unknown age sample. The values of 'J' obtained are in the interval of 2.85 a 3.03 (x 10 - 3)J/h. Rocks from 'Tres Virgenes' were dated by the method described in this work, showing an agreement with previous values of different authors. The age of this rocks are from Cenozoico era, mainly in the miocene period. (Author)

40 CFR 40.145-2 - Federal Water Pollution Control Act.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Federal Water Pollution Control Act. 40... FEDERAL ASSISTANCE RESEARCH AND DEMONSTRATION GRANTS § 40.145-2 Federal Water Pollution Control Act. (a... or control of acid or other mine water pollution; and (2) That the State shall provide legal and...

40 CFR 40.140-3 - Federal Water Pollution Control Act.

Science.gov (United States)

2010-07-01

... such safe water and such elimination or control of water pollution for all native villages in the State... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Federal Water Pollution Control Act. 40... FEDERAL ASSISTANCE RESEARCH AND DEMONSTRATION GRANTS § 40.140-3 Federal Water Pollution Control Act. (a...

Energy dependence of identified hadron spectra and event-by-event fluctuations in p+p interactions from NA61/SHINE at the CERN SPS

CERN Document Server

Rybczynski, Maciej; Aduszkiewicz, A.; Ali, Y.; Anticic, T.; Antoniou, N.; Argyriades, J.; Baatar, B.; Blondel, A.; Blumer, J.; Bogomilov, M.; Bravar, A.; Brooks, W.; Brzychczyk, J.; Bubak, A.; Bunyatov, S.A.; Busygina, O.; Christakoglou, P.; Czopowicz, T.; Davis, N.; Debieux, S.; Dembinski, H.; Diakonos, F.; Di Luise, S.; Dominik, W.; Drozhzhova, T.; Dumarchez, J.; Dynowski, K.; Engel, R.; Ereditato, A.; Esposito, L.S.; Feofilov, G.A.; Fodor, Z.; Ferrero, A.; Fulop, A.; Gazdzicki, M.; Golubeva, M.; Grabez, B.; Grebieszkow, K.; Grzeszczuk, A.; Guber, F.; Hakobyan, H.; Hasegawa, T.; Hierholzer, M.; Idczak, R.; Igolkin, S.; Ivanov, Y.; Ivashkin, A.; Jakovic, D.; Kadija, K.; Kapoyannis, A.; Katrynska, N.; Kaptur, E.; Kielczewska, D.; Kikola, D.; Kirejczyk, M.; Kisiel, J.; Kiss, T.; Kleinfelder, S.; Kobayashi, T.; Kolesnikov, V.I.; Kolev, D.; Kondratiev, V.P.; Korzenev, A.; Kowalski, S.; Krasnoperov, A.; Kuleshov, S.; Kurepin, A.; Larsen, D.; Laszlo, A.; Lyubushkin, V.V.; Mackowiak-Pawlowska, M.; Majka, Z.; Maksiak, B.; Malakhov, A.I.; Maletic, D.; Marchionni, A.; Marcinek, A.; Marin, V.; Marton, K.; Mathes, H.J.; Matulewicz, T.; Matveev, V.; Melkumov, G.L.; Mrowczynski, St.; Murphy, S.; Nakadaira, T.; Nirkko, M.; Nishikawa, K.; Palczewski, T.; Palla, G.; Panagiotou, A.D.; Paul, T.; Pistillo, C.; Redij, A.; Peryt, W.; Petukhov, O.; Planeta, R.; Pluta, J.; Popov, B.A.; Posiadala, M.; Pulawski, S.; Puzovic, J.; Rauch, W.; Ravonel, M.; Renfordt, R.; Robert, A.; Röhrich, D.; Rondio, E.; Roth, M.; Rubbia, A.; Rustamov, A.; Rybczynski, M.; Sadovsky, A.; Sakashita, K.; Savic, M.; Sekiguchi, T.; Seyboth, P.; Shibata, M.; Sipos, M.; Skrzypczak, E.; Slodkowski, M.; Staszel, P.; Stefanek, G.; Stepaniak, J.; Stroebele, H.; Susa, T.; Szuba, M.; Tada, M.; Tereshchenko, V.; Tolyhi, T.; Tsenov, R.; Turko, L.; Ulrich, R.; Unger, M.; Vassiliou, M.; Veberic, D.; Vechernin, V.V.; Vesztergombi, G.; Vinogradov, L.; Wilczek, A.; Wlodarczyk, Z.; Wojtaszek, A.; Wyszynski, O.; Zambelli, L.; Zipper, W.

2013-01-01

NA61/SHINE at the CERN SPS is a fixed-target experiment pursuing a rich physics program including measurements for heavy ion, neutrino and cosmic ray physics. The main goal of the ion program is to explore the most interesting $T, mu_{B}$ region of the phase diagram of strongly interacting matter. We plan to study the properties of the onset of deconfinement and to search for the signatures of the critical point. The search is performed by varying collision energy (13A-158A GeV/c) and system size (p+p, Be+Be, Ar+Ca, Xe+La). Thanks to its large acceptance and excellent particle identification capability NA61/SHINE is well suited for performing high-precision particle production measurements as well as for studying event-by-event fluctuations in p+p, p+nucleus and nucleus+nucleus collisions. Preliminary results on p+p interactions at 20, 31, 40, 80 and 158 GeV/c are presented. They include inclusive spectra of pi+, pi-, K- and protons as a function of transverse momentum/mass and rapidity as well as event-by-ev...

Effects of cell condition, pH, and temperature on lead, zinc, and copper sorption to Acidithiobacillus caldus strain BC13

International Nuclear Information System (INIS)

Aston, John E.; Apel, William A.; Lee, Brady D.; Peyton, Brent M.

2010-01-01

Research highlights: →At. caldus sorbs lead, zinc, and copper across a range of pH and temperature. →At. caldus shows a relatively high sorption capacity for zinc and copper at low pH. → Lead, zinc, and copper sorption decreases in tertiary mixtures. → Copper appears to sorb via a different mechanism(s) than lead or zinc. - Abstract: This study describes the effects of cell condition, pH, and temperature on lead, zinc, and copper sorption to Acidithiobacillus caldus strain BC13 with a Langmuir model. Copper exhibited the highest loading capacity, 4.76 ± 0.28 mmol g -1 , to viable cells at pH 5.5. The highest k L (binding-site affinity) observed was 61.2 ± 3.0 L mmol -1 to dehydrated cells at pH 4.0. The pHs that maximized loading capacities and binding-site affinities were generally between 4.0 and 5.5, where the sum of free-proton and complexed-metal concentrations was near a minimum. Of additional importance, lead, zinc, and copper sorbed to viable cells at pH values as low as 1.5. Previous studies with other acidithiobacilli did not measure viable-cell sorption below pH 4.0. In separate experiments, desorption studies showed that far less copper was recovered from viable cells than any other metal or cell condition, suggesting that uptake may play an important role in copper sorption by At. caldus strain BC13. To reflect an applied system, the sorption of metal mixtures was also studied. In these experiments, lead, zinc, and copper sorption from a tertiary mixture were 40.2 ± 4.3%, 28.7 ± 3.8%, and 91.3 ± 3.0%, respectively, of that sorbed in single-metal systems.

anti-p p --> pi0 pi0 from 600-MeV/c to 1940-MeV/c

CERN Document Server

Bugg, D V

1999-01-01

Crystal Barrel data on pbarp -> pi sup 0 pi sup 0 are presented. Shapes of angular distributions are similar to data of Dulude et al, but show some systematic disagreements. Absolute normalisations disagree seriously. An amplitude analysis finds two alternative solutions. Both contain a J sup P sup C 2 sup + sup + resonance with M = 2020 +- 40 MeV and GAMMA = 235 +- 70 MeV, tentatively identified as the qbarq sup 3 F sub 2 state. Both solutions also require f sub 4 (2050) and f sub 4 (2300).

Response of Syntrophic Propionate Degradation to pH Decrease and Microbial Community Shifts in an UASB Reactor.

Science.gov (United States)

Zhang, Liguo; Ban, Qiaoying; Li, Jianzheng; Jha, Ajay Kumar

2016-08-28

The effect of pH on propionate degradation in an upflow anaerobic sludge blanket (UASB) reactor containing propionate as a sole carbon source was studied. Under influent propionate of 2,000 mg/l and 35ºC, propionate removal at pH 7.5-6.8 was above 93.6%. Propionate conversion was significantly inhibited with stepwise pH decrease from pH 6.8 to 6.5, 6.0, 5.5, 5.0, 4.5, and then to 4.0. After long-term operation, the propionate removal at pH 6.5-4.5 maintained an efficiency of 88.5%-70.1%, whereas propionate was hardly decomposed at pH 4.0. Microbial composition analysis showed that propionate-oxidizing bacteria from the genera Pelotomaculum and Smithella likely existed in this system. They were significantly reduced at pH ≤5.5. The methanogens in this UASB reactor belonged to four genera: Methanobacterium, Methanospirillum, Methanofollis, and Methanosaeta. Most detectable hydrogenotrophic methanogens were able to grow at low pH conditions (pH 6.0-4.0), but the acetotrophic methanogens were reduced as pH decreased. These results indicated that propionate-oxidizing bacteria and acetotrophic methanogens were more sensitive to low pH (5.5-4.0) than hydrogenotrophic methanogens.

40 CFR 721.3821 - L-Glutamic acid, N-(1-oxododecyl)-.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false L-Glutamic acid, N-(1-oxododecyl... Substances § 721.3821 L-Glutamic acid, N-(1-oxododecyl)-. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified as L-Glutamic acid, N-(1-oxododecyl)- (PMN P...

Identification of a Novel Methylated Gene in Nasopharyngeal Carcinoma: TTC40

Directory of Open Access Journals (Sweden)

Wajdi Ayadi

2014-01-01

Full Text Available To further explore the epigenetic changes in nasopharyngeal carcinoma (NPC, methylation-sensitive arbitrarily primed PCR was performed on NPC biopsies and nontumor nasopharyngeal samples. We have shown mainly two DNA fragments that appeared to be differentially methylated in NPCs versus nontumors. The first, defined as hypermethylated, corresponds to a CpG island at the 5′-end of the tetratricopeptide repeat domain 40 (TTC40 gene, whereas the second, defined as hypo-methylated, is located on repetitive sequences at chromosomes 16p11.1 and 13.1. Thereafter, the epigenetic alteration on the 5′-TTC40 gene was confirmed by methylation-specific PCR, showing a significant aberrant methylation in NPCs, compared to nontumors. In addition, the bisulfite sequencing analysis has shown a very high density of methylated cytosines in C15, C17, and X666 NPC xenografts. To assess whether TTC40 gene is silenced by aberrant methylation, we examined the gene expression by reverse transcription-PCR. Our analysis showed that the mRNA expression was significantly lower in tumors than in nontumors, which is associated with 5′-TTC40 gene hypermethylation. In conclusion, we found that the 5′-TTC40 gene is frequently methylated and is associated with the loss of mRNA expression in NPCs. Hypermethylation of 5′-TTC40 gene might play a role in NPC development; nevertheless, other studies are needed.

Micro Electrochemical pH Sensor Applicable for Real-Time Ratiometric Monitoring of pH Values in Rat Brains.

Science.gov (United States)

Zhou, Jie; Zhang, Limin; Tian, Yang

2016-02-16

To develop in vivo monitoring meter for pH measurements is still the bottleneck for understanding the role of pH plays in the brain diseases. In this work, a selective and sensitive electrochemical pH meter was developed for real-time ratiometric monitoring of pH in different regions of rat brains upon ischemia. First, 1,2-naphthoquinone (1,2-NQ) was employed and optimized as a selective pH recognition element to establish a 2H(+)/2e(-) approach over a wide range of pH from 5.8 to 8.0. The pH meter demonstrated remarkable selectivity toward pH detection against metal ions, amino acids, reactive oxygen species, and other biological species in the brain. Meanwhile, an inner reference, 6-(ferrocenyl)hexanethiol (FcHT), was selected as a built-in correction to avoid the environmental effect through coimmobilization with 1,2-NQ. In addition, three-dimensional gold nanoleaves were electrodeposited onto the electrode surface to amplify the signal by ∼4.0-fold and the measurement was achieved down to 0.07 pH. Finally, combined with the microelectrode technique, the microelectrochemical pH meter was directly implanted into brain regions including the striatum, hippocampus, and cortex and successfully applied in real-time monitoring of pH values in these regions of brain followed by global cerebral ischemia. The results demonstrated that pH values were estimated to 7.21 ± 0.05, 7.13 ± 0.09, and 7.27 ± 0.06 in the striatum, hippocampus, and cortex in the rat brains, respectively, in normal conditions. However, pH decreased to 6.75 ± 0.07 and 6.52 ± 0.03 in the striatum and hippocampus, upon global cerebral ischemia, while a negligible pH change was obtained in the cortex.

Sulfate Reduction at pH 4.0 for Treatment of Process and Wastewaters

NARCIS (Netherlands)

Bijmans, M.F.M.; Vries, de E.; Yang, C.H.; Buisman, C.J.N.; Lens, P.N.L.; Dopson, M.

2010-01-01

Acidic industrial process and wastewaters often contain high sulfate and metal concentrations and their direct biological treatment is thus far not possible as biological processes at pH <5 have been neglected. Sulfate-reducing bacteria convert sulfate to sulfide that can subsequently be used to

pH-sensitive liposomes: characterization and application

International Nuclear Information System (INIS)

Connor, J.

1986-01-01

It has been demonstrated that liposomes composed of dioleoylphosphatidylethanolamine (DOPE) and palmitoylhomocysteine (PHC) have the ability to fuse with adjacent membranes upon exposure to mildly acid pH. The ability of liposomes to fuse is absolutely dependent on the presence of DOPE and a weakly acidic amphiphile. The acid induced fusion event is a leaky process, but the leakage can be reduced by 50%, with only a small loss of fusion ability, by the inclusion of 40 mole percent cholesterol. Using an established monoclonal antibody targeting system. pH-sensitive immunoliposomes were prepared which successfully delivered entrapped calcein to the cytoplasm of target cells. The addition of chloroquine, which raises the internal pH of cellular vacuoles, blocks the cytoplasmic delivery of the pH-sensitive immunoliposomes. pH-insensitive immunoliposomes delivered calcein only to the endosome/lysosome system and not the cytoplasm. 31 P-NMR and light scattering of DOPE:OA liposomes under acidic conditions demonstrate that the effect of the protons and the divalent cations is to force the DOPE to revert to the hexagonal II configuration. In vivo experiments with DOPE:OA immunoliposomes indicate that the liposomes rapidly aggregate and release their contents upon exposure to plasma. These results indicate that pH-sensitive immunoliposomes are an effective tool for in vitro cytoplasmic delivery but are ineffective for in vivo delivery at this point in development

Polarization transfer in (p,n) reactions at 495 MeV

International Nuclear Information System (INIS)

Taddeucci, T.N.

1991-01-01

Polarization transfer observables have been measured with the NTOF facility at LAMPF for (p,n) reactions at 495 MeV. Measurements of the longitudinal polarization transfer parameter D LL for transitions to discrete states at 0 degrees show convincing evidence for tensor interaction effects. Complete sets of polarization transfer observables have been measured for quasifree (p,n) reactions on 2 H, 12 C, 40 Ca at a scattering angle of 18 degrees. These measurements show no evidence for an enhancement in the isovector spin longitudinal response. 19 refs., 10 figs

40 CFR 721.2097 - Azo chromium complex dyestuff preparation (generic name).

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Azo chromium complex dyestuff... New Uses for Specific Chemical Substances § 721.2097 Azo chromium complex dyestuff preparation... substance identified generically as an azo chromium complex dyestuff preparation (PMN P-95-240) is subject...

40 CFR 721.10098 - Disubstituted benzoic acid, alkali metal salt (generic).

Science.gov (United States)

2010-07-01

... New Uses for Specific Chemical Substances § 721.10098 Disubstituted benzoic acid, alkali metal salt... identified generically as disubstituted benzoic acid, alkali metal salt (PMN P-03-643) is subject to... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Disubstituted benzoic acid, alkali...

40 CFR 721.3620 - Fatty acid amine condensate, polycarboxylic acid salts.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Fatty acid amine condensate... Specific Chemical Substances § 721.3620 Fatty acid amine condensate, polycarboxylic acid salts. (a... a fatty acid amine condensate, polycarboxylic acid salts. (PMN P-92-445) is subject to reporting...

PROTEKSI MINYAK IKAN LEMURU, MINYAK KELAPA SAWIT, DAN BUNGKIL SAWIT TERHADAP pH DAN NH3DALAM RUMEN SAPI PERANAKAN ONGOLE

Directory of Open Access Journals (Sweden)

Catur Suci Purwati

2016-03-01

Full Text Available The aimed of this research is to know the influence of protection lemuru oil, palm oil, and palm oil cake to pH and NH3in the rumen of the Ongole. The material were used in this study werecomulated rumenfemale cows with average body weight of 289.33 ± 28.34 kg as many as 3 heads. Latin square experiment design was applied on 3 treatments. Fermented rice straw (FRS, basal concentrate (BC, and protected materials of Indian sardine oil (ISO, palm oil (PO, and palmkernel cake (PKC were used as a feed ingredient.  Treatments were: P1 = FRS 40% + BC 60% (BC 95% + PO 5%; P2 = FRS 40% + BC 60% (BC 95% + ISO 5% ; P3 = FRS 40% + BC 60% (BC 90% + PKC 10%. Parameters measured were pH and NH3. Latin square experiment design was applied on 3 treatments.Conclusions of this study are pH and NH3remain stable, meaning lemuru addition of fish oil, palm oil, and palm oil cake is protected not disturb the digestive process in the cow rumen fistulated onggole breedparticular. (Key word: Indian sardine oil, NH3, Palm kernel cake, Palm oil, pH, Protection

Biological effects of brachytherapy using a 32P-patch on the skin of Sencar mice

International Nuclear Information System (INIS)

Salgueiro, Maria J.; Medina, Vanina; Zubillaga, Marcela

2008-01-01

In recent years, specially designed patches containing beta emitters have been developed for contact brachytherapy of skin lesions. The aim of the present work is to evaluate the biological effects of the 32 P-patch on the skin of Sencar mice as a result of a brachytherapy treatment. For this purpose, a 32 P-patch was prepared with Chromic 32 P-phosphate and silicone and the classical model of two-stage skin carcinogenesis was reproduced in Sencar mice. Animals were divided in two main groups in order to perform the contact brachytherapy treatment using schemes of single (SD40 and SD60) and fractionated (FD40 and FD60) doses, with their respective control groups (CSD and CFD). Additionally, a control group without carcinogenic treatment was included in order to apply the 32 P-patch in normal skin. The endpoint to evaluate treatment effects was tumor size after a follow-up period of 44 days and finally, animals were sacrificed in order to get samples of all tumors for histological analysis. Additionally, PCNA staining was evaluated in all groups and the biologically effective dose (BED) of each scheme was calculated taken into account the linear-quadratic model. Erythema, dermatitis and skin ulceration developed in almost all treated animals, but they gradually healed with regeneration of tissue during the follow-up period. Radiation effects on the skin of SD40, SD60, FD40 and FD60 showed a significant reduction of the tumor size with regard to controls, independently of the scheme and the radiation dose considered. PCNA staining scores of groups in the single dose scheme resulted higher for control than for treated tumors, and the same pattern was observed for groups of the fractionated dose scheme. This radioactive 32 P-silicone-patch which, is easy to prepare and use in the treatment of skin diseases and seems promissory as a radioactive device for clinical use. (author)

Atmospheric Retrievals of HAT-P-16b and WASP-11b/HAT-P-10b

Science.gov (United States)

McIntyre, Kathleen; Harrington, Joseph; Challener, Ryan; Lenius, Maria; Hartman, Joel D.; Bakos, Gaspar A.; Blecic, Jasmina; Cubillos, Patricio E.; Cameron, Andrew

2018-01-01

We report Bayesian atmospheric retrievals performed on the exoplanets HAT-P-16b and WASP-11b/HAT-P-10b. HAT-P-16b is a hot (equilibrium temperature 1626 ± 40 K, assuming zero Bond albedo and efficient energy redistribution), 4.19 ± 0.09 Jupiter-mass exoplanet orbiting an F8 star every 2.775960 ± 0.000003 days (Buchhave et al 2010). WASP-11b/HAT-P-10b is a cooler (1020 ± 17 K), 0.487 ± 0.018 Jupiter-mass exoplanet orbiting a K3 star every 3.7224747 ± 0.0000065 days (Bakos et al. 2009, co-discovered by West et al. 2008). We observed secondary eclipses of both planets using the 3.6 μm and 4.5 μm channels of the Spitzer Space Telescope's Infrared Array Camera (program ID 60003). We applied our Photometry for Orbits, Eclipses, and Transits (POET) code to produce normalized eclipse light curves, and our Bayesian Atmospheric Radiative Transfer (BART) code to constrain the temperature-pressure profiles and atmospheric molecular abundances of the two planets. Spitzer is operated by the Jet Propulsion Laboratory, California Institute of Technology, under a contract with NASA. This work was supported by NASA Planetary Atmospheres grant NNX12AI69G and NASA Astrophysics Data Analysis Program grant NNX13AF38G.

Optimization of a pH-shift control strategy for producing monoclonal antibodies in Chinese hamster ovary cell cultures using a pH-dependent dynamic model.

Science.gov (United States)

Hogiri, Tomoharu; Tamashima, Hiroshi; Nishizawa, Akitoshi; Okamoto, Masahiro

2018-02-01

To optimize monoclonal antibody (mAb) production in Chinese hamster ovary cell cultures, culture pH should be temporally controlled with high resolution. In this study, we propose a new pH-dependent dynamic model represented by simultaneous differential equations including a minimum of six system component, depending on pH value. All kinetic parameters in the dynamic model were estimated using an evolutionary numerical optimization (real-coded genetic algorithm) method based on experimental time-course data obtained at different pH values ranging from 6.6 to 7.2. We determined an optimal pH-shift schedule theoretically. We validated this optimal pH-shift schedule experimentally and mAb production increased by approximately 40% with this schedule. Throughout this study, it was suggested that the culture pH-shift optimization strategy using a pH-dependent dynamic model is suitable to optimize any pH-shift schedule for CHO cell lines used in mAb production projects. Copyright © 2017 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Characterization of GmENOD40, a gene showing novel patterns of cell-specific expression during soybean nodule development.

NARCIS (Netherlands)

Yang, W.C.; Katinakis, P.; Hendriks, P.; Smolders, A.; Vries, de F.; Spee, J.; Kammen, van A.; Bisseling, T.; Franssen, H.

1993-01-01

In this paper, the soybean 'early nodulin' clone pGmENOD40 is characterized. The GmENOD40 encoded protein does not contain methionine and does not show homology to proteins identified so far. In situ hybridizations showed that this gene has a complex expression pattern during development of

Detection of D2-40 monoclonal antibody-labeled lymphatic vessel invasion in esophageal squamous cell carcinoma and its clinicopathologic significance

International Nuclear Information System (INIS)

Bai, Bing; Ma, Wei; Wang, Kai; Ha, Sita; Wang, Jian-Bo; Tan, Bing-Xu; Wang, Na-Na; Yang, Sheng-Si; Jia, Yi-Bin; Cheng, Yu-Feng

2013-01-01

This study aims to investigate the clinicopathologic significance of lymphatic vessel invasion (LVI) labeled by D2-40 monoclonal antibody in esophageal squamous cell carcinoma (ESCC). Immunohistochemical assay was used to detect the expression of D2-40 and LVI in 107 ESCC patients. Then, the correlation between the clinicopathologic feature and the overall survival time of the patients was analyzed. The lymph node metastasis rates were 70% and 21% in the LVI-positive and LVI-negative groups, respectively. The nodal metastasis rate was higher in the LVI-positive group than in the LVI-negative group. Multivariate regression analysis showed that LVI was related to nodal metastasis (P<0.001). The median survival time of the patients was 26 and 43 months in the LVI-positive and LVI-negative groups, respectively. Although univariate regression analysis showed significant difference between the two groups (P=0.014), multivariate regression analysis revealed that LVI was not an independent prognostic factor for overall survival in the ESCC patients (P=0.062). Lymphatic node metastasis (P=0.031), clinical stage (P=0.019), and residual tumor (P=0.026) were the independent prognostic factors. LVI labeled by D2-40 monoclonal antibody is a risk factor predictive of lymph node metastasis in ESCC patients

40 CFR 464.40 - Applicability; description of the zinc casting subcategory.

Science.gov (United States)

2010-07-01

... casting subcategory. 464.40 Section 464.40 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS METAL MOLDING AND CASTING POINT SOURCE CATEGORY Zinc Casting Subcategory § 464.40 Applicability; description of the zinc casting subcategory. The provisions of this...

p,p'-Dichlorodiphenyltrichloroethane (p,p'-DDT) and p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) repress prostate specific antigen levels in human prostate cancer cell lines.

Science.gov (United States)

Wong, Lilian I L; Labrecque, Mark P; Ibuki, Naokazu; Cox, Michael E; Elliott, John E; Beischlag, Timothy V

2015-03-25

Despite stringent restrictions on their use by many countries since the 1970s, the endocrine disrupting chemicals, DDT and DDE are still ubiquitous in the environment. However, little attention has been directed to p,p'-DDT and the anti-androgen, p,p'-DDE on androgen receptor (AR) target gene transcription in human cells. Inhibitors of androgenic activity may have a deleterious clinical outcome in prostate cancer screens and progression, therefore we determined whether environmentally relevant concentrations of p,p'-DDT and p,p'-DDE negatively impact AR-regulated expression of prostate-specific antigen (PSA), and other AR target genes in human LNCaP and VCaP prostate cancer cells. Quantitative real-time PCR and immuno-blotting techniques were used to measure intracellular PSA, PSMA and AR mRNA and protein levels. We have shown for the first time that p,p'-DDT and p,p'-DDE repressed R1881-inducible PSA mRNA and protein levels in a dose-dependent manner. Additionally, we used the fully automated COBAS PSA detection system to determine that extracellular PSA levels were also significantly repressed. These chemicals achieve this by blocking the recruitment of AR to the PSA promoter region at 10 μM, as demonstrated by the chromatin immunoprecipitation (ChIP) in LNCaP cells. Both p,p'-DDT and p,p'-DDE repressed R1881-inducible AR protein accumulation at 10 μM. Thus, we conclude that men who have been exposed to either DDT or DDE may produce a false-negative PSA test when screening for prostate cancer, resulting in an inaccurate clinical diagnosis. More importantly, prolonged exposure to these anti-androgens may mimic androgen ablation therapy in individuals with prostate cancer, thus exacerbating the condition by inadvertently forcing adaptation to this stress early in the disease. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

TEOLOGIA DA LIBERTAÇÃO: 40 ANOS

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. Editorial

2011-01-01

Full Text Available Algo inédito! Em tão pouco tempo, a Teologia da Libertação (TdL passou por vicissitudes que as outras o fizeram ao longo de séculos. Medimos as teologias por longa duração. E a TdL tem apenas 40 anos que, em termos de história, significa brevíssimo lapso de tempo. E que balanço fazer desse périplo tão breve?

An Investigation of Lower Urinary Tract Symptoms in Women Aged 40 and Over.

Science.gov (United States)

Sever, Neziha; Oskay, Umran

2017-01-01

The objective of this study was to determine lower urinary tract symptoms (LUTS) and associated risk factors in women aged 40 years and over. The study was carried out with a total of 312 women. The data were collected between 1 January 2009 and 30 July 2010. As research instruments, an interview form of 19 questions that questioned personal characteristics and was developed by researchers, and the Bristol Female Lower Urinary Tract Symptoms (BFLUTS) Questionnaire evaluating lower urinary tract symptoms were used. Linear regression analysis was used to detect associated risk factors. The rates of urgency, urinary incontinence, nocturia, and frequency symptoms were 61.5, 52.2, 18.9, and 25%, respectively. BFLUTS total scores increased with age, but the present study has detected no statistically significant differences (P > 0.05). BFLUTS scores of the women demonstrated statistically significant differences according to several risk factors including menopause status (P = 0.03), presence of chronic illness (P = 0.000), medicine use (P = 0.000), recurrent urinary tract infections (P = 0.000), body mass index (BMI) (P = 0.004), delivery number (P = 0.005) and chronic constipation (P = 0.002). Multiple linear regression analysis determined that frequent urinary tract infections, presence of chronic illness, chronic constipation, BMI and number of deliveries were significantly related to LUTS development. The most common LUTS was urgency in women aged 40 years and older. Recurrent urinary tract infection was determined as the most significant risk factor for LUTS, followed by chronic illness, chronic constipation, higher BMI and parity. © 2015 Wiley Publishing Asia Pty Ltd.