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Sample records for oz fleets phospho

  1. The definition of kernel Oz

    OpenAIRE

    Smolka, Gert

    1994-01-01

    Oz is a concurrent language providing for functional, object-oriented, and constraint programming. This paper defines Kernel Oz, a semantically complete sublanguage of Oz. It was an important design requirement that Oz be definable by reduction to a lean kernel language. The definition of Kernel Oz introduces three essential abstractions: the Oz universe, the Oz calculus, and the actor model. The Oz universe is a first-order structure defining the values and constraints Oz computes with. The ...

  2. SN 2006oz

    DEFF Research Database (Denmark)

    Leloudas, Georgios; Chatzopoulos, E.; Dilday, B.

    2012-01-01

    to contribute to a better understanding of these objects by studying SN 2006oz, a newly-recognized member of this class. Methods. We present multi-color light curves of SN 2006oz from the SDSS-II SN Survey that cover its rise time, as well as an optical spectrum that shows that the explosion occurred at z ~ 0.......376. We fitted black-body functions to estimate the temperature and radius evolution of the photosphere and used the parametrized code SYNOW to model the spectrum. We constructed a bolometric light curve and compared it with explosion models. In addition, we conducted a deep search for the host galaxy...... to a recombination wave in a circumstellar medium (CSM) and discuss whether this is a common property of all similar explosions. The subsequent rise can be equally well described by input from a magnetar or by ejecta-CSM interaction, but the models are not well constrained owing to the lack of post...

  3. PhosphoSiteAnalyzer

    DEFF Research Database (Denmark)

    Bennetzen, Martin V; Cox, Jürgen; Mann, Matthias

    2012-01-01

    an algorithm to retrieve kinase predictions from the public NetworKIN webpage in a semiautomated way and applies hereafter advanced statistics to facilitate a user-tailored in-depth analysis of the phosphoproteomic data sets. The interface of the software provides a high degree of analytical flexibility......Phosphoproteomic experiments are routinely conducted in laboratories worldwide, and because of the fast development of mass spectrometric techniques and efficient phosphopeptide enrichment methods, researchers frequently end up having lists with tens of thousands of phosphorylation sites...... and is designed to be intuitive for most users. PhosphoSiteAnalyzer is a freeware program available at http://phosphosite.sourceforge.net ....

  4. OZ: An Innovative Primary Flight Display, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — The proposed SBIR project will develop OZ, an innovative primary flight display for aircraft. The OZ display, designed from "first principles" of vision science,...

  5. Improving fleet performance

    International Nuclear Information System (INIS)

    Ramjist, S.

    2015-01-01

    Use Fleet Initiatives to Improve Overall Fleet Performance . Tightly Integrated with Business Planning (Cause & Effect) . Leverage Strength of Broader Organization - Converge on Standard Business Practices . Ancillary Benefit of Improved Agility.

  6. Stalin's Big Fleet Program

    National Research Council Canada - National Science Library

    Mauner, Milan

    2002-01-01

    Although Dr. Milan Hauner's study 'Stalin's Big Fleet program' has focused primarily on the formation of Big Fleets during the Tsarist and Soviet periods of Russia's naval history, there are important lessons...

  7. Improving fleet performance

    Energy Technology Data Exchange (ETDEWEB)

    Ramjist, S. [Ontario Power Generation, Toronto, ON (Canada)

    2015-07-01

    Use Fleet Initiatives to Improve Overall Fleet Performance . Tightly Integrated with Business Planning (Cause & Effect) . Leverage Strength of Broader Organization - Converge on Standard Business Practices . Ancillary Benefit of Improved Agility.

  8. FleetDASH

    Energy Technology Data Exchange (ETDEWEB)

    Singer, Mark R [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

    2017-09-06

    FleetDASH helps federal fleet managers maximize their use of alternative fuel. This presentation explains how the dashboard works and demonstrates the newest capabilities added to the tool. It also reviews complementary online tools available to fleet managers on the Alternative Fuel Data Center.

  9. Liner Shipping Fleet Repositioning

    DEFF Research Database (Denmark)

    Tierney, Kevin; Jensen, Rune Møller

    2011-01-01

    Liner shipping fleet repositioning consists of moving vessels between services in a liner ship- ping network in order to better orient the overall network to the world economy, and to ensure the proper maintenance of vessels. Thus, fleet repositioning involves sailing and loading activities subject...

  10. Maximizing your fleet

    Energy Technology Data Exchange (ETDEWEB)

    Lytle, J. [GE Capital Rail Services, Calgary, AB (Canada)

    2001-07-01

    A series of viewgraphs were presented to illustrate this discussion which focused on the economics of the railroad environment in 2001 and beyond. Fleet productivity and customer relations were described. The viewgraphs were entitled: new car builds; fleet demographics for North American LPG/AA fleet; and, issues and trends of North American LPG/AA fleet. It was noted that there is a continued demand for North American LPG/AA fleet as cars are phased out. GE Capital Rail Services is addressing the future by focusing on the following four initiatives: service, globalization, building a six sigma company, and transforming into an e-business. The company's six sigma approach is based on customer orientation, safety and compliance, quality and reliability, productivity and planning, cost, and people and culture. The actions taken thus far have been a planned maintenance program, the relocation of safety valves, and the elimination of early failures. figs.

  11. Fleet Feedback and Fleet Efficiency Metrics

    Energy Technology Data Exchange (ETDEWEB)

    Singer, Mark R [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

    2017-09-05

    The Marine Corps have 10 years of experience implementing a telematics program and several lessons to share with partner agencies. This presentation details results of a Marine Corps survey as well as methods of using telematics to promote fleet efficiency and optimize the vehicle acquisition process.

  12. Federal Fleet Report

    Data.gov (United States)

    General Services Administration — Annual report of Federal agencies' motor vehicle fleet data collected in the Federal Automotive Statistical Tool (FAST), a web-based reporting tool cosponsored by...

  13. Fleet management performance monitoring.

    Science.gov (United States)

    2013-05-01

    The principle goal of this project was to enhance and expand the analytical modeling methodology previously developed as part of the Fleet Management Criteria: Disposal Points and Utilization Rates project completed in 2010. The enhanced and ex...

  14. Fishing fleet profiling methodology

    National Research Council Canada - National Science Library

    Ferraris, Jocelyne

    2002-01-01

    A fishing fleet profile aims tho assist in understanding the complexity and structure of fisheries from a technical and socio-economic point of view, or from the point of view of fishing strategies...

  15. Fleet DNA (Presentation)

    Energy Technology Data Exchange (ETDEWEB)

    Walkokwicz, K.; Duran, A.

    2014-06-01

    The Fleet DNA project objectives include capturing and quantifying drive cycle and technology variation for the multitude of medium- and heavy-duty vocations; providing a common data storage warehouse for medium- and heavy-duty vehicle fleet data across DOE activities and laboratories; and integrating existing DOE tools, models, and analyses to provide data-driven decision making capabilities. Fleet DNA advantages include: for Government - providing in-use data for standard drive cycle development, R&D, tech targets, and rule making; for OEMs - real-world usage datasets provide concrete examples of customer use profiles; for fleets - vocational datasets help illustrate how to maximize return on technology investments; for Funding Agencies - ways are revealed to optimize the impact of financial incentive offers; and for researchers -a data source is provided for modeling and simulation.

  16. The Effects of Dr. Oz on Health Behaviors and Attitudes

    Science.gov (United States)

    Crouch, Elizabeth; Dickes, Lori A.; Davis, Amanda; Zarandy, Joy

    2016-01-01

    Background: Consumption of social media has quickly evolved into a primary source of health information for many consumers. This seems to be particularly true for individuals seeking to modify chronic health conditions like weight loss, obesity, and obesity-related diseases. Purpose: This study explores whether watching Dr. Oz weight loss episodes…

  17. FLEET ASSIGNMENT MODELLING

    Directory of Open Access Journals (Sweden)

    2016-01-01

    Full Text Available The article is devoted to the airline scheduling process and methods of its modeling. This article describes the main stages of airline scheduling process (scheduling, fleet assignment, revenue management, operations, their features and interactions. The main part of scheduling process is fleet assignment. The optimal solution of the fleet assignment problem enables airlines to increase their incomes up to 3 % due to quality improving of connections and execution of the planned number of flights operated by less number of aircraft than usual or planned earlier. Fleet assignment of scheduling process is examined and Conventional Leg-Based Fleet Assignment Model is analyzed. Finally strong and weak aspects of the model (SWOT are released and applied. The article gives a critical analysis of FAM model, with the purpose of identi- fying possible options and constraints of its use (for example, in cases of short-term and long-term planning, changing the schedule or replacing the aircraft, as well as possible ways to improve the model.

  18. Fleet DNA Brings Fleet Data to Life, Informs R&D | News | NREL

    Science.gov (United States)

    Fleet DNA Brings Fleet Data to Life, Informs R&D Fleet DNA Brings Fleet Data to Life, Informs R De La Rosa, NREL 34672 The Fleet DNA clearinghouse of commercial vehicle operations data features Odyne-have tapped into Fleet DNA." The data-driven insight and decision-making capabilities

  19. OzBot and haptics: remote surveillance to physical presence

    Science.gov (United States)

    Mullins, James; Fielding, Mick; Nahavandi, Saeid

    2009-05-01

    This paper reports on robotic and haptic technologies and capabilities developed for the law enforcement and defence community within Australia by the Centre for Intelligent Systems Research (CISR). The OzBot series of small and medium surveillance robots have been designed in Australia and evaluated by law enforcement and defence personnel to determine suitability and ruggedness in a variety of environments. Using custom developed digital electronics and featuring expandable data busses including RS485, I2C, RS232, video and Ethernet, the robots can be directly connected to many off the shelf payloads such as gas sensors, x-ray sources and camera systems including thermal and night vision. Differentiating the OzBot platform from its peers is its ability to be integrated directly with haptic technology or the 'haptic bubble' developed by CISR. Haptic interfaces allow an operator to physically 'feel' remote environments through position-force control and experience realistic force feedback. By adding the capability to remotely grasp an object, feel its weight, texture and other physical properties in real-time from the remote ground control unit, an operator's situational awareness is greatly improved through Haptic augmentation in an environment where remote-system feedback is often limited.

  20. PhosphoBase: a database of phosphorylation sites

    DEFF Research Database (Denmark)

    Blom, Nikolaj; Kreegipuu, Andres; Brunak, Søren

    1998-01-01

    PhosphoBase is a database of experimentally verified phosphorylation sites. Version 1.0 contains 156 entries and 398 experimentally determined phosphorylation sites. Entries are compiled and revised from the literature and from major protein sequence databases such as SwissProt and PIR. The entries...... provide information about the phosphoprotein and the exact position of its phosphorylation sites. Furthermore, part of the entries contain information about kinetic data obtained from enzyme assays on specific peptides. To illustrate the use of data extracted from PhosphoBase we present a sequence logo...... displaying the overall conservation of positions around serines phosphorylated by protein kinase A (PKA). PhosphoBase is available on the WWW at http://www.cbs.dtu.dk/databases/PhosphoBase/....

  1. Data of evolutionary structure change: 2OZ2A-4PADA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 2OZ2A-4PADA 2OZ2 4PAD A A APAAVDWRARGAVTAVKDQGQCGSCWAFSAIGNVECQWF.../entryChain> 4PAD A 4PADA...n> 4PAD A 4PADA VVLQ...4PAD A 4PADA RIKRGTGNSYGVCG A 4PADA LVAQY---GIHYR

  2. OzFlux data: network integration from collection to curation

    Directory of Open Access Journals (Sweden)

    P. Isaac

    2017-06-01

    Full Text Available Measurement of the exchange of energy and mass between the surface and the atmospheric boundary-layer by the eddy covariance technique has undergone great change in the last 2 decades. Early studies of these exchanges were confined to brief field campaigns in carefully controlled conditions followed by months of data analysis. Current practice is to run tower-based eddy covariance systems continuously over several years due to the need for continuous monitoring as part of a global effort to develop local-, regional-, continental- and global-scale budgets of carbon, water and energy. Efficient methods of processing the increased quantities of data are needed to maximise the time available for analysis and interpretation. Standardised methods are needed to remove differences in data processing as possible contributors to observed spatial variability. Furthermore, public availability of these data sets assists with undertaking global research efforts. The OzFlux data path has been developed (i to provide a standard set of quality control and post-processing tools across the network, thereby facilitating inter-site integration and spatial comparisons; (ii to increase the time available to researchers for analysis and interpretation by reducing the time spent collecting and processing data; (iii to propagate both data and metadata to the final product; and (iv to facilitate the use of the OzFlux data by adopting a standard file format and making the data available from web-based portals. Discovery of the OzFlux data set is facilitated through incorporation in FLUXNET data syntheses and the publication of collection metadata via the RIF-CS format. This paper serves two purposes. The first is to describe the data sets, along with their quality control and post-processing, for the other papers of this Special Issue. The second is to provide an example of one solution to the data collection and curation challenges that are encountered by similar flux

  3. Cask fleet operations study

    International Nuclear Information System (INIS)

    1988-01-01

    The Nuclear Waste Policy Act of 1982 assigned to the Department of Energy's (DOE) Office of Civilian Waste Management the responsibility for disposing of high-level waste and spent fuel. A significant part of that responsibility involves transporting nuclear waste materials within the federal waste management system; that is, from the waste generator to the repository. The lead responsibility for transportation operations has been assigned to Oak Ridge Operations, with Oak Ridge National Laboratory (ORNL) providing technical support through the Transportation Operations Support Task Group. One of the ORNL support activities involves assessing what facilities, equipment and services are required to assure that an acceptable, cost-effective and safe transportation operations system can be designed, operated and maintained. This study reviews, surveys and assesses the experience of Nuclear Assurance Corporation (NAC) in operating a fleet of spent-fuel shipping casks to aid in developing the spent-fuel transportation system

  4. Fleet retrofit report

    Science.gov (United States)

    1973-01-01

    Flight tests are evaluated of an avionics system which aids the pilot in making two-segment approaches for noise abatement. The implications are discussed of equipping United's fleet of Boeing 727-200 aircraft with two-segment avionics for use down to Category 2 weather operating minima. The experience is reported of incorporating two-segment approach avionics systems on two different aircraft. The cost of installing dual two-segment approach systems is estimated to be $37,015 per aircraft, including parts, labor, and spares. This is based on the assumption that incremental out-of-service and training costs could be minimized by incorporating the system at airframe overhaul cycle and including training in regular recurrent training. Accelerating the modification schedule could add up to 50 percent to the modification costs. Recurring costs of maintenance of the installation are estimated to be of about the same magnitude as the potential recurrent financial benefits due to fuel savings.

  5. Fleet DNA Brings Fleet Data to Life, Informs R&D | NREL

    Science.gov (United States)

    Fleet DNA Brings Fleet Data to Life, Informs R&D Science and Technology Highlights Highlights in Research & Development Fleet DNA Brings Fleet Data to Life, Informs R&D Key Research Results Achievement Built on over 11.5 million miles of vehicle operations data, Fleet DNA helps users

  6. PhosProtect - a novel and superior compound to tag and protect phospho-groups during mass spectrometry based phospho-proteomics

    DEFF Research Database (Denmark)

    2012-01-01

    Value Proposition The PhosProtect compound alone (developed and tested, IP secured)* • protects phospho-groups during tandem mass spectrometry, thus reducing problematic neutral loss of phosphate. • provides unique phospho-tag by causing isotopic distribution patterns in MS and MS/MS data. The PhosProtect...... compound covalently bound to column material (in progress , IP secured)** • Combines enrichment, protection and tagging of phospho-peptides and phospho-lipids in one easy workflow....

  7. Fleet management for autonomous vehicles

    OpenAIRE

    Bsaybes, Sahar; Quilliot, Alain; Wagler, Annegret K.

    2016-01-01

    The VIPAFLEET project consists in developing models and algorithms for man- aging a fleet of Individual Public Autonomous Vehicles (VIPA). Hereby, we consider a fleet of cars distributed at specified stations in an industrial area to supply internal transportation, where the cars can be used in different modes of circulation (tram mode, elevator mode, taxi mode). One goal is to develop and implement suitable algorithms for each mode in order to satisfy all the requests under an economic point...

  8. Comparative Analysis of Conventional Electronic and OZ Concept Displays for Aircraft Energy Management

    Science.gov (United States)

    Baker, Erik Reese

    A repeated-measures, within-subjects design was conducted on 58 participant pilots to assess mean differences on energy management situation awareness response time and response accuracy between a conventional electronic aircraft display, a primary flight display (PFD), and an ecological interface design aircraft display, the OZ concept display. Participants were associated with a small Midwestern aviation university, including student pilots, flight instructors, and faculty with piloting experience. Testing consisted of observing 15 static screenshots of each cockpit display type and then selecting applicable responses from 27 standardized responses for each screen. A paired samples t-test was computed comparing accuracy and response time for the two displays. There was no significant difference in means between PFD Response Time and OZ Response Time. On average, mean PFD Accuracy was significantly higher than mean OZ Accuracy (MDiff = 13.17, SDDiff = 20.96), t(57) = 4.78, p performance differences were not operationally remarkable. There was no significant correlation between PFD Response Time and PFD Accuracy, but there was a significant correlation between OZ Response Time and OZ Accuracy, r (58) = .353, p performing as well as experienced professional pilots on dynamic flight tasks with the OZ display. A demographic questionnaire and a feedback survey were included in the trial. An equivalent three-quarters majority of participants rated the PFD as "easy" and the OZ as "confusing", yet performance accuracy and response times between the two displays were not operationally different.

  9. A distinctive patchy osteomalacia characterises Phospho1-deficient mice.

    Science.gov (United States)

    Boyde, Alan; Staines, Katherine A; Javaheri, Behzad; Millan, Jose Luis; Pitsillides, Andrew A; Farquharson, Colin

    2017-08-01

    The phosphatase PHOSPHO1 is involved in the initiation of biomineralisation. Bones in Phospho1 knockout (KO) mice show histological osteomalacia with frequent bowing of long bones and spontaneous fractures: they contain less mineral, with smaller mineral crystals. However, the consequences of Phospho1 ablation on the microscale structure of bone are not yet fully elucidated. Tibias and femurs obtained from wild-type and Phospho1 null (KO) mice (25-32 weeks old) were embedded in PMMA, cut and polished to produce near longitudinal sections. Block surfaces were studied using 20 kV backscattered-electron (BSE) imaging, and again after iodine staining to reveal non-mineralised matrix and cellular components. For 3D characterisation, we used X-ray micro-tomography. Bones opened with carbide milling tools to expose endosteal surfaces were macerated using an alkaline bacterial pronase enzyme detergent, 5% hydrogen peroxide and 7% sodium hypochlorite solutions to produce 3D surfaces for study with 3D BSE scanning electron microscopy (SEM). Extensive regions of both compact cortical and trabecular bone matrix in Phospho1 KO mice contained no significant mineral and/or showed arrested mineralisation fronts, characterised by a failure in the fusion of the calcospherite-like, separately mineralising, individual micro-volumes within bone. Osteoclastic resorption of the uncalcified matrix in Phospho1 KO mice was attenuated compared with surrounding normally mineralised bone. The extent and position of this aberrant biomineralisation varied considerably between animals, contralateral limbs and anatomical sites. The most frequent manifestation lay, however, in the nearly complete failure of mineralisation in the bone surrounding the numerous transverse blood vessel canals in the cortices. In conclusion, SEM disclosed defective mineralising fronts and extensive patchy osteomalacia, which has previously not been recognised. These data further confirm the role of this phosphatase

  10. H/OZ: PFD and Collaborative Flight Control System, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Researchers at the Institute for Human and Machine Cognition invented OZ, a primary flight display that provides a single, unified graphic display of critical flight...

  11. Strategic vehicle fleet management - the composition problem

    Directory of Open Access Journals (Sweden)

    Adam Redmer

    2015-03-01

    Full Text Available Background: Fleets constitute the most important production means in transportation. Their appropriate management is crucial for all companies having transportation duties. The paper is the second one of a series of three papers that the author dedicates to the strategic vehicle fleet management topic. Material and methods: The paper discusses ways of building companies' fleets of vehicles. It means deciding on the number of vehicles in a fleet (the fleet sizing problem - FS and types of vehicles in a fleet (the fleet composition problem - FC. The essence of both problems lies in balancing transportation supply and demand taking into account different demand types to be fulfilled and different vehicle types that can be put into a fleet. Vehicles, which can substitute each other while fulfilling different demand types. In the paper an original mathematical model (an optimization method allowing for the FS/FC analysis is proposed. Results: An application of the proposed optimization method in a real-life decision situation (the case study within the Polish environment and the obtained solution are presented. The solution shows that there exist some best fitted (optimal fleet size / composition matching company's transportation requirements. An optimal fleet size / composition allows for a significantly higher fleet utilization (10-15% higher than any other, including random fleet structure. Moreover, any changes in the optimal fleet size / composition, even small ones, result in a lower utilization of vehicles (lower by a few percent. Conclusions: The presented in this paper analysis, on the one hand, is consistent with a widespread opinion that the number of vehicle types in a fleet should be limited. In the other words it means that the versatility / interchangeability of vehicles is very important. On the other hand, the analysis proves that even small changes in a fleet size / fleet composition can result in an important changes of the fleet

  12. Anomaly Detection in a Fleet of Systems

    Data.gov (United States)

    National Aeronautics and Space Administration — A fleet is a group of systems (e.g., cars, aircraft) that are designed and manufactured the same way and are intended to be used the same way. For example, a fleet...

  13. National Clean Fleets Partnership (Fact Sheet)

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2014-01-01

    Clean Cities' National Clean Fleets Partnership establishes strategic alliances with large fleets to help them explore and adopt alternative fuels and fuel economy measures to cut petroleum use. The initiative leverages the strength of nearly 100 Clean Cities coalitions, nearly 18,000 stakeholders, and more than 20 years of experience. It provides fleets with top-level support, technical assistance, robust tools and resources, and public acknowledgement to help meet and celebrate fleets' petroleum-use reductions.

  14. Time to retire : Indicators for aircraft fleets

    NARCIS (Netherlands)

    Newcamp, Jeffrey; Verhagen, W.J.C.; Curran, R.

    2017-01-01

    It is well known that aircraft fleets are aging alongside rising operations and support costs. Logisticians and fleet managers who better understand the milestones and timeline of an aging fleet can recognise potential savings. This paper outlines generalised milestones germane to military aircraft

  15. Nuclear fleet: Today and tomorrow

    International Nuclear Information System (INIS)

    Levin, B.M.; Kovalenko, V.K.; Sinyaev, A.K.

    1993-01-01

    Many years of operational experience have shown advantages of nuclear liner icebreakers over those burning fossil fuels primarily in ensuring reliable and stable shipping along the North Sea Route with an extended navigation season being realized. The advantages of the nuclear fleet are described

  16. National Clean Fleets Partnership (Fact Sheet)

    Energy Technology Data Exchange (ETDEWEB)

    2012-01-01

    Provides an overview of Clean Cities National Clean Fleets Partnership (NCFP). The NCFP is open to large private-sector companies that have fleet operations in multiple states. Companies that join the partnership receive customized assistance to reduce petroleum use through increased efficiency and use of alternative fuels. This initiative provides fleets with specialized resources, expertise, and support to successfully incorporate alternative fuels and fuel-saving measures into their operations. The National Clean Fleets Partnership builds on the established success of DOE's Clean Cities program, which reduces petroleum consumption at the community level through a nationwide network of coalitions that work with local stakeholders. Developed with input from fleet managers, industry representatives, and Clean Cities coordinators, the National Clean Fleets Partnership goes one step further by working with large private-sector fleets.

  17. The phosphoCTD-interacting domain of Topoisomerase I

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Jianhong; Phatnani, Hemali P.; Hsieh, Tao-Shih [Department of Biochemistry, Duke University Medical Center, Durham, NC 27710 (United States); Greenleaf, Arno L., E-mail: arno.greenleaf@duke.edu [Department of Biochemistry, Duke University Medical Center, Durham, NC 27710 (United States)

    2010-06-18

    The N-terminal domain (NTD) of Drosophila melanogaster (Dm) Topoisomerase I has been shown to bind to RNA polymerase II, but the domain of RNAPII with which it interacts is not known. Using bacterially-expressed fusion proteins carrying all or half of the NTDs of Dm and human (Homo sapiens, Hs) Topo I, we demonstrate that the N-terminal half of each NTD binds directly to the hyperphosphorylated C-terminal repeat domain (phosphoCTD) of the largest RNAPII subunit, Rpb1. Thus, the amino terminal segment of metazoan Topo I (1-157 for Dm and 1-114 for Hs) contains a novel phosphoCTD-interacting domain that we designate the Topo I-Rpb1 interacting (TRI) domain. The long-known in vivo association of Topo I with active genes presumably can be attributed, wholly or in part, to the TRI domain-mediated binding of Topo I to the phosphoCTD of transcribing RNAPII.

  18. The phosphoCTD-interacting domain of Topoisomerase I

    International Nuclear Information System (INIS)

    Wu, Jianhong; Phatnani, Hemali P.; Hsieh, Tao-Shih; Greenleaf, Arno L.

    2010-01-01

    The N-terminal domain (NTD) of Drosophila melanogaster (Dm) Topoisomerase I has been shown to bind to RNA polymerase II, but the domain of RNAPII with which it interacts is not known. Using bacterially-expressed fusion proteins carrying all or half of the NTDs of Dm and human (Homo sapiens, Hs) Topo I, we demonstrate that the N-terminal half of each NTD binds directly to the hyperphosphorylated C-terminal repeat domain (phosphoCTD) of the largest RNAPII subunit, Rpb1. Thus, the amino terminal segment of metazoan Topo I (1-157 for Dm and 1-114 for Hs) contains a novel phosphoCTD-interacting domain that we designate the Topo I-Rpb1 interacting (TRI) domain. The long-known in vivo association of Topo I with active genes presumably can be attributed, wholly or in part, to the TRI domain-mediated binding of Topo I to the phosphoCTD of transcribing RNAPII.

  19. SimPhospho: a software tool enabling confident phosphosite assignment.

    Science.gov (United States)

    Suni, Veronika; Suomi, Tomi; Tsubosaka, Tomoya; Imanishi, Susumu Y; Elo, Laura L; Corthals, Garry L

    2018-03-27

    Mass spectrometry combined with enrichment strategies for phosphorylated peptides has been successfully employed for two decades to identify sites of phosphorylation. However, unambiguous phosphosite assignment is considered challenging. Given that site-specific phosphorylation events function as different molecular switches, validation of phosphorylation sites is of utmost importance. In our earlier study we developed a method based on simulated phosphopeptide spectral libraries, which enables highly sensitive and accurate phosphosite assignments. To promote more widespread use of this method, we here introduce a software implementation with improved usability and performance. We present SimPhospho, a fast and user-friendly tool for accurate simulation of phosphopeptide tandem mass spectra. Simulated phosphopeptide spectral libraries are used to validate and supplement database search results, with a goal to improve reliable phosphoproteome identification and reporting. The presented program can be easily used together with the Trans-Proteomic Pipeline and integrated in a phosphoproteomics data analysis workflow. SimPhospho is available for Windows, Linux and Mac operating systems at https://sourceforge.net/projects/simphospho/. It is open source and implemented in C ++. A user's manual with detailed description of data analysis using SimPhospho as well as test data can be found as supplementary material of this article. Supplementary data are available at https://www.btk.fi/research/ computational-biomedicine/software/.

  20. A cask fleet operations study

    International Nuclear Information System (INIS)

    1988-03-01

    This document describes the cask fleet currently available to transport spent nuclear fuels. The report describes the proposed operational procedures for these casks and the vehicles intended to transport them. Included are techniques for loading the cask, lifting it onto the transport vehicle, preparing the invoices, and unloading the cask at the destination. The document concludes with a discussion on the maintenance and repair of the casks. (tem) 29 figs

  1. OzDES multifibre spectroscopy for the Dark Energy Survey: first-year operation and results

    Energy Technology Data Exchange (ETDEWEB)

    Yuan, Fang; Lidman, C.; Davis, T. M.; Childress, M.; Abdalla, F. B.; Banerji, M.; Buckley-Geer, E.; Carnero Rosell, A.; Carollo, D.; Castander, F. J.; D' Andrea, C. B.; Diehl, H. T.; Cunha, C. E.; Foley, R. J.; Frieman, J.; Glazebrook, K.; Gschwend, J.; Hinton, S.; Jouvel, S.; Kessler, R.; Kim, A. G.; King, A. L.; Kuehn, K.; Kuhlmann, S.; Lewis, G. F.; Lin, H.; Martini, P.; McMahon, R. G.; Mould, J.; Nichol, R. C.; Norris, R. P.; O' Neill, C. R.; Ostrovski, F.; Papadopoulos, A.; Parkinson, D.; Reed, S.; Romer, A. K.; Rooney, P. J.; Rozo, E.; Rykoff, E. S.; Sako, M.; Scalzo, R.; Schmidt, B. P.; Scolnic, D.; Seymour, N.; Sharp, R.; Sobreira, F.; Sullivan, M.; Thomas, R. C.; Tucker, D.; Uddin, S. A.; Wechsler, R. H.; Wester, W.; Wilcox, H.; Zhang, B.; Abbott, T.; Allam, S.; Bauer, A. H.; Benoit-L?vy, A.; Bertin, E.; Brooks, D.; Burke, D. L.; Carrasco Kind, M.; Covarrubias, R.; Crocce, M.; da Costa, L. N.; DePoy, D. L.; Desai, S.; Doel, P.; Eifler, T. F.; Evrard, A. E.; Fausti Neto, A.; Flaugher, B.; Fosalba, P.; Gaztanaga, E.; Gerdes, D.; Gruen, D.; Gruendl, R. A.; Honscheid, K.; James, D.; Kuropatkin, N.; Lahav, O.; Li, T. S.; Maia, M. A. G.; Makler, M.; Marshall, J.; Miller, C. J.; Miquel, R.; Ogando, R.; Plazas, A. A.; Roodman, A.; Sanchez, E.; Scarpine, V.; Schubnell, M.; Sevilla-Noarbe, I.; Smith, R. C.; Soares-Santos, M.; Suchyta, E.; Swanson, M. E. C.; Tarle, G.; Thaler, J.; Walker, A. R.

    2015-07-29

    We present results for the first three years of OzDES, a six-year program to obtain redshifts for objects in the Dark Energy Survey (DES) supernova fields using the 2dF fibre positioner and AAOmega spectrograph on the Anglo-Australian Telescope. OzDES is a multi-object spectroscopic survey targeting multiple types of targets at multiple epochs over a multi-year baseline, and is one of the first multi-object spectroscopic surveys to dynamically include transients into the target list soon after their discovery. At the end of three years, OzDES has spectroscopically confirmed almost 100 supernovae, and has measured redshifts for 17,000 objects, including the redshifts of 2,566 supernova hosts. We examine how our ability to measure redshifts for targets of various types depends on signal-to-noise, magnitude, and exposure time, finding that our redshift success rate increases significantly at a signal-to-noise of 2 to 3 per 1-A° ngstrom bin. We also find that the change in signal-to-noise with exposure time closely matches the Poisson limit for stacked exposures as long as 10 hours.We use these results to predict the redshift yield of the full OzDES survey, as well as the potential yields of future surveys on other facilities such as 4MOST, PFS, and MSE. This work marks the first OzDES data release, comprising 15,327 redshifts. OzDES is on target to obtain over 30,000 redshifts over the six-year duration of the survey, including a yield of approximately 5,700 supernova host-galaxy redshifts.

  2. Strategic vehicle fleet management - the replacement problem

    Directory of Open Access Journals (Sweden)

    Adam Redmer

    2016-03-01

    Full Text Available Background: Fleets constitute the most important production means in transportation. Their appropriate management is crucial for all companies having transportation duties. The paper is the third one of a series of three papers that the author dedicates to the strategic vehicle fleet management topic. Material and methods: The paper discusses ways of building replacement strategies for companies' fleets of vehicles. It means deciding for how long to exploit particular vehicles in a fleet (the fleet replacement problem - FR. The essence of this problem lies in the minimization of vehicle / fleet exploitation costs by balancing ownership and utilization costs and taking into account budget limitations. In the paper an original mathematical model (an optimization method allowing for the FR analysis is proposed. Results: An application of the proposed optimization method in a real-life decision situation (the case study within the Polish environment and the obtained solution are presented. The solution shows that there exist optimal exploitation periods of particular vehicles in a fleet. However, combination of them gives a replacement plan for an entire fleet violating budget constraints. But it is possible to adjust individual age to replacement of particular vehicles to fulfill budget constraints without losing economical optimality of a developed replacement plan for an entire fleet. Conclusions: The paper is the last one of a series of three papers that the author dedicated to the strategic vehicle fleet management topic including the following managerial decision problems: MAKE-or-BUY, sizing / composition and replacement.

  3. Commercial vehicle fleet management and information systems. Technical memorandum 3 : ITS fleet management technology resource guide

    Science.gov (United States)

    1997-05-01

    In todays increasingly competitive economic environment, effective management of commercial vehicle fleets is important for all types of carriers and for the trucking industry as a whole. To meet fleet management needs, carriers increasingly are t...

  4. Fleet Aviation Maintenance Organic Support (FAMOS) Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — Purpose:The Fleet Aviation Maintenance Organic Support (FAMOS) Laboratory at the Naval Air Warfare Center Aircraft Division, Lakehurst, NJ provides rapid engineering...

  5. Elaboration and Characterization of Nano-Sized AlxMoyOz/Al Thermites

    National Research Council Canada - National Science Library

    Comet, M; Spitzer, D

    2006-01-01

    ...) has been developed at the Institut franco-allemand de recherches de Saint Louis (ISL). This process consists of a new sol-gel method nano-sized mixed AlxMoyOz phases whose structure is correlated to the chemical composition...

  6. Judas, Verrader of de trouwste leerling? : Theologische kanttekeningen bij een roman van Amos Oz

    NARCIS (Netherlands)

    Koet, Bart

    2017-01-01

    Judas, one of Jesus’ disciples, has been increasingly rehabilitated over the last few years. His gospel has been rediscovered. Interesting books are written about his role in Scriptures and in history. Novelist Amos Oz has written his own unique contribution to the debate. This Israeli writer has

  7. Alternative fuelds in urban fleets

    Energy Technology Data Exchange (ETDEWEB)

    Lindsay, T.

    1994-12-31

    In this presentation the author addresses four main objectives. They are to: discuss programs that are driving the introduction of alternative fuels into fleet operations in urban areas around the country; define alternative fuels; quantify the present use and future projections on alternative fuel vehicles (AVFs) in the Chicago metropolitan statistical area; and discuss benefits of increased use of alternative fuels in urban areas. Factors which touch on these points include: present domestic dependence on petroleum for autos, with usage exceeding production; the large populations in urban areas which do not meet Clean Air Standards; recent legislative initiatives which give guidance and aid in the adoption of such strategies.

  8. Alternative fuelds in urban fleets

    International Nuclear Information System (INIS)

    Lindsay, T.

    1994-01-01

    In this presentation the author addresses four main objectives. They are to: discuss programs that are driving the introduction of alternative fuels into fleet operations in urban areas around the country; define alternative fuels; quantify the present use and future projections on alternative fuel vehicles (AVFs) in the Chicago metropolitan statistical area; and discuss benefits of increased use of alternative fuels in urban areas. Factors which touch on these points include: present domestic dependence on petroleum for autos, with usage exceeding production; the large populations in urban areas which do not meet Clean Air Standards; recent legislative initiatives which give guidance and aid in the adoption of such strategies

  9. An introduction to the Australian and New Zealand flux tower network - OzFlux

    Science.gov (United States)

    Beringer, Jason; Hutley, Lindsay B.; McHugh, Ian; Arndt, Stefan K.; Campbell, David; Cleugh, Helen A.; Cleverly, James; Resco de Dios, Víctor; Eamus, Derek; Evans, Bradley; Ewenz, Cacilia; Grace, Peter; Griebel, Anne; Haverd, Vanessa; Hinko-Najera, Nina; Huete, Alfredo; Isaac, Peter; Kanniah, Kasturi; Leuning, Ray; Liddell, Michael J.; Macfarlane, Craig; Meyer, Wayne; Moore, Caitlin; Pendall, Elise; Phillips, Alison; Phillips, Rebecca L.; Prober, Suzanne M.; Restrepo-Coupe, Natalia; Rutledge, Susanna; Schroder, Ivan; Silberstein, Richard; Southall, Patricia; Yee, Mei Sun; Tapper, Nigel J.; van Gorsel, Eva; Vote, Camilla; Walker, Jeff; Wardlaw, Tim

    2016-10-01

    OzFlux is the regional Australian and New Zealand flux tower network that aims to provide a continental-scale national research facility to monitor and assess trends, and improve predictions, of Australia's terrestrial biosphere and climate. This paper describes the evolution, design, and current status of OzFlux as well as provides an overview of data processing. We analyse measurements from all sites within the Australian portion of the OzFlux network and two sites from New Zealand. The response of the Australian biomes to climate was largely consistent with global studies except that Australian systems had a lower ecosystem water-use efficiency. Australian semi-arid/arid ecosystems are important because of their huge extent (70 %) and they have evolved with common moisture limitations. We also found that Australian ecosystems had a similar radiation-use efficiency per unit leaf area compared to global values that indicates a convergence toward a similar biochemical efficiency. The two New Zealand sites represented extremes in productivity for a moist temperate climate zone, with the grazed dairy farm site having the highest GPP of any OzFlux site (2620 gC m-2 yr-1) and the natural raised peat bog site having a very low GPP (820 gC m-2 yr-1). The paper discusses the utility of the flux data and the synergies between flux, remote sensing, and modelling. Lastly, the paper looks ahead at the future direction of the network and concludes that there has been a substantial contribution by OzFlux, and considerable opportunities remain to further advance our understanding of ecosystem response to disturbances, including drought, fire, land-use and land-cover change, land management, and climate change, which are relevant both nationally and internationally. It is suggested that a synergistic approach is required to address all of the spatial, ecological, human, and cultural challenges of managing the delicately balanced ecosystems in Australasia.

  10. The BRCT domain is a phospho-protein binding domain.

    Science.gov (United States)

    Yu, Xiaochun; Chini, Claudia Christiano Silva; He, Miao; Mer, Georges; Chen, Junjie

    2003-10-24

    The carboxyl-terminal domain (BRCT) of the Breast Cancer Gene 1 (BRCA1) protein is an evolutionarily conserved module that exists in a large number of proteins from prokaryotes to eukaryotes. Although most BRCT domain-containing proteins participate in DNA-damage checkpoint or DNA-repair pathways, or both, the function of the BRCT domain is not fully understood. We show that the BRCA1 BRCT domain directly interacts with phosphorylated BRCA1-Associated Carboxyl-terminal Helicase (BACH1). This specific interaction between BRCA1 and phosphorylated BACH1 is cell cycle regulated and is required for DNA damage-induced checkpoint control during the transition from G2 to M phase of the cell cycle. Further, we show that two other BRCT domains interact with their respective physiological partners in a phosphorylation-dependent manner. Thirteen additional BRCT domains also preferentially bind phospho-peptides rather than nonphosphorylated control peptides. These data imply that the BRCT domain is a phospho-protein binding domain involved in cell cycle control.

  11. State and Alternative Fuel Provider Fleets - Fleet Compliance Annual Report: Model Year 2015, Fiscal Year 2016

    Energy Technology Data Exchange (ETDEWEB)

    2016-12-01

    The U.S. Department of Energy (DOE) regulates covered state government and alternative fuel provider fleets, pursuant to the Energy Policy Act of 1992 (EPAct), as amended. Covered fleets may meet their EPAct requirements through one of two compliance methods: Standard Compliance or Alternative Compliance. For model year (MY) 2015, the compliance rate with this program for the more than 3011 reporting fleets was 100%. More than 294 fleets used Standard Compliance and exceeded their aggregate MY 2015 acquisition requirements by 8% through acquisitions alone. The seven covered fleets that used Alternative Compliance exceeded their aggregate MY 2015 petroleum use reduction requirements by 46%.

  12. NATIONAL FLEET DEVELOPMENT IN THE INNOVATIVE ECONOMY. CASE STUDY OF THE GREEK FLEET

    Directory of Open Access Journals (Sweden)

    Marek Grzybowski

    2016-06-01

    Full Text Available In the article the development of the Greek fleet in the period of last ten years was discussed. The Greek fleet is an example of accommodating itself to the requirements of the global and innovative economy. Greek shipowners are developing their fleets through the consolidation and replacing older ships with new generation vessels. They are invest-ing into ships adapted for new markets, including LNG maritime transport market. As a result of it Greece became a market leader in maritime transport sector and their fleet a biggest and youngest fleet of world in 2016.

  13. BioFleet case studies

    International Nuclear Information System (INIS)

    2007-01-01

    These six case studies examined the use of different biodiesel blends as fuel supply sources for businesses in British Columbia (BC). In the first case study, 6 municipalities participated in a pilot program designed to compare the performance of biodiesel and diesel fuels. Each municipality operated 2 base vehicles running on conventional diesel along with 2 similar vehicles which used biodiesel. Real time emissions tests and analyses of the vehicles using biodiesel were also conducted by 2 of the participating municipalities. All municipalities participating in the study agreed to purchase significant volumes of biodiesel. The second case study described a pilot study conducted by the City of Vancouver's equipment services branch in 2004. As a result of the study, the city now has over 530 types of equipment that use biodiesel. The third case study described a program designed by TSI Terminals in Vancouver to assess the emission reduction impact of using biodiesel at its port facility. Six different pieces of equipment were used to confirm that biodiesel could be used throughout the terminal. Test results confirmed that biodiesel blends could be used to reduce emissions. Overall emissions were reduced by 30 per cent. The fourth case study described a waste renderer that used a fleet of 36 trucks to deliver raw products to its plants. The company made the decision to use only biodiesel for its entire fleet of trucks. Since July 2005, the company has logged over 1.7 million km using biodiesel blends. The fifth case study described a salmon hatchery that switched from diesel to biodiesel in order to reduce emissions. The biodiesel blends are used to fuel the hatchery's 2 diesel generators. The hatchery has reduced emissions of greenhouse gases (GHGs) by an estimated 1800 tonnes annually. The sixth case study described how the Township of Langley has started using biodiesel for its entire fleet of of approximately 250 pieces of equipment. The township has not

  14. Usurping the Apocryphal: Antonio Muñoz Molina's Cosmopolitan Memory of Max Aub's Rhetoric of Testimony

    OpenAIRE

    Aguirre Oteiza, Daniel

    2017-01-01

    This paper focuses on a recent gesture by the Spanish novelist and public intellectual Antonio Muñoz Molina to publicly recuperate Aub’s testimony of uprootedness in France, Algeria, and Mexico, a gesture that relies on the concept of cosmopolitan memory as it has been developed in Holocaust studies. I critique Muñoz Molina’s use of the Holocaust as a template for a transnational cultural history based on a supposedly shared Jewish past. Muñoz Molina generalizes on two levels: he turns Aub in...

  15. Guanylate kinase domains of the MAGUK family scaffold proteins as specific phospho-protein-binding modules

    OpenAIRE

    Zhu, Jinwei; Shang, Yuan; Xia, Caihao; Wang, Wenning; Wen, Wenyu; Zhang, Mingjie

    2011-01-01

    Membrane-associated guanylate kinases (MAGUK) family proteins contain an inactive guanylate kinase (GK) domain, whose function has been elusive. Here, this domain is revealed as a new type of phospho-peptide-binding module, in which the GMP-binding site has evolved to accommodate phospho-serines or -threonines.

  16. RATADA DE ROSABETTY MUÑOZ: METÁFORAS DE UN TIEMPO CRUEL

    Directory of Open Access Journals (Sweden)

    Iván Carrasco M

    2006-11-01

    Full Text Available Este trabajo interpreta el libro Ratada, de la poeta Rosabetty Muñoz, como metáforas (alegoría de la sociedad chilena durante la dictadura militar. Leída de este modo, Ratada es una escritura terapéutica, pues provoca en la conciencia del lector un efecto de dolor equivalente al de la limpieza de las heridas, necesaria para que el cuerpo saneThis paper interprets the book Ratada, written by the poet Rosabetty Muñoz, as metaphors (allegory for the chilean society during the military dictatorship. In this reading, Ratada becomes therapeutic writing, as it provokes in the reader's mind an effect of pain equivalent to the cleansing of wounds, necessary for the healing of the body

  17. La imagen dentro de sí. Sobre tres obras de Oscar Muñoz

    Directory of Open Access Journals (Sweden)

    Paola Cortes Rocca

    2015-12-01

    Full Text Available Oscar Muñoz's work revolves around the question of the specificity of photography. By doing so, it redirects a current trend of visual studies that are fascinated with the non-specificity of the objects and the itinerancy of languages. Muñoz wonders what lies at the heart of photography. Is it the mechanical reproduction or the ability to chemically fix the image? Is it the power to isolate the instant or to perpetuate anything captured by the machine? The transformation that introduces photography has less to do with the work itself and more with the experience of image. That experience is, first of all, an experience of time since the image produces an intermediate time between proliferation and what is left as a remain.

  18. Amós Oz: de lealdades e traições

    Directory of Open Access Journals (Sweden)

    Saul Kirschbaum

    2015-11-01

    Full Text Available Este artigo analisará Judas, o mais recente romance (2014 do escritor israelense Amós Oz. Nessa obra de ficção, Oz utiliza o contexto da suposta traição do apóstolo Judas, narrada nos Evangelhos, e o delicado relacionamento afetivo entre um jovem universitário e uma viúva de guerra de meia idade para abordar as intensas controvérsias no interior do movimento sionista nos anos que antecederam a criação do Estado de Israel, com veementes acusações de traição contra os militantes que se opuseram à linha hegemônica da Organização Sionista e da Agência Judaica – propunham a criação de um estado binacional – e tentativas de deslegitimá-los.

  19. The Case of Dr. Oz: Ethics, Evidence, and Does Professional Self-Regulation Work?

    Science.gov (United States)

    Tilburt, Jon C; Allyse, Megan; Hafferty, Frederic W

    2017-02-01

    Dr. Mehmet Oz is widely known not just as a successful media personality donning the title "America's Doctor ® ," but, we suggest, also as a physician visibly out of step with his profession. A recent, unsuccessful attempt to censure Dr. Oz raises the issue of whether the medical profession can effectively self-regulate at all. It also raises concern that the medical profession's self-regulation might be selectively activated, perhaps only when the subject of professional censure has achieved a level of public visibility. We argue here that the medical profession must look at itself with a healthy dose of self-doubt about whether it has sufficient knowledge of or handle on the less visible Dr. "Ozes" quietly operating under the profession's presumptive endorsement. © 2017 American Medical Association. All Rights Reserved.

  20. Los realismos en Beatus Ille de Antonio Muñoz Molina.

    Directory of Open Access Journals (Sweden)

    Papa Mamour Diop

    2007-07-01

    Full Text Available ENGLISH: This work deals with the current debate about reality and its literary and artistic expression: realism. In Beatus Ille, Antonio Muñoz Molina’s first novel, the fictional universe reveals three kinds of realism: the epic and elegiac realism, the historic realismand the magic realism. SPANISH: En este trabajo, abordamos el actual debate sobre la realidad y su expresión literaria y artística: el realismo. En Beatus Ille, primera novela de Antonio Muñoz Molina, eluniverso novelesco permite percibir tres tipos de realismos: el realismo épico elegiaco, el realismo histórico o contrafactual y el realismo mágico.

  1. MARS-OZ - A Design for a Simulated Mars Base in the Australian Outback

    Science.gov (United States)

    Willson, D.; Clarke, J. D. A.; Murphy, G.

    Mars Society Australia has developed the design of a simulated Mars base, MARS-OZ, for deployment in outback Australia. MARS-OZ will provide a platform for a diverse range of Mars analogue research in Australia. The simulated base consists of two mobile modules whose dimensions and shape approximate those of horizontally landed bent biconic spacecraft described in an earlier paper. The modules are designed to support field engineering, robotics, architectural, geological, biological and human factors research at varying levels of simulation fidelity. Non-Mars related research can also be accommodated, for example general field geology and biology, and engineering research associated with sustainable, low impact architecture. Crews of up to eight can be accommodated. In addition to its research function, the base also will serve as a centre of space education and outreach activities. The prime site for the MARS-OZ simulated base is located in the northern Flinders Ranges near Arkaroola in South Australia. This region contains many features that provide useful scientific analogues to known or possible past and present conditions on Mars from both a geological and biological perspective. The features will provide a wealth of study opportunities for crews. The very diverse terrain and regolith materials will provide ideal opportunities to field trial a range of equipment, sensors and exploration strategies. If needed, the prime site can be secured from casual visitors, allowing research into human interaction in isolation. Despite its relative isolation, the site is readily accessible by road and air from major Australian centres. This paper provides description of the configuration, design and construction of the proposed facility, its interior layout, equipment and systems fitouts, a detailed cost estimate, and its deployment. We estimate that the deployment of MARS-OZ could occur within nine months of securing funding.

  2. Moderno/Posmoderno/Neomoderno: El jinete polaco, de Antonio Muñoz Molina

    Directory of Open Access Journals (Sweden)

    Marta Beatriz Ferrari

    2014-06-01

    Full Text Available Antonio Muñoz Molina (Úbeda, 1956 es ya, sin lugar a dudas, uno de los más destacados novelistas españoles contemporáneos. boga en la década que va de 1960 a 1970 al “realismo depurado” y al compromiso ético. La narrativa de Muñoz Molina apela constantemente a los cruces de códigos y al hacerlo va adoptando singulares modulaciones; itinerario que en su recorrido irá dando cuenta de un posicionamiento ideológico frente a los fenómenos derivados de la industria cultural –fenómenos camp y kitsch–, los géneros menores, la intertextualidad y lo intermedial. En esta curva, El jinete polaco significaría un punto de inflexión dentro de la producción narrativa del autor. En ella Muñoz Molina escribe una novela inspirada todavía en un motivo pictórico, pero abandona la astucia narrativa del final sorprendente y combina múltiples peripecias, historias cruzadas, diversos puntos de vista que apuntan a construir algo semejante a lo que Mario Vargas Llosa intuyó en Cien años de soledad de García Márquez, una “novela total”.

  3. Pressurized HxCyOz Cells at ca. 250 °C: Potential and Challenges

    DEFF Research Database (Denmark)

    Mogensen, Mogens Bjerg; Chatzichristodoulou, Christodoulos; Allebrod, Frank

    2013-01-01

    focus on cells that may have a potential of forming or using HxCyOz in electrolysis or fuel cell mode, respectively. Examples of HxCyOz are hydrogen with (x,y,z) = (2,0,0), carbon monoxide with (x,y,z) = (0,1,1), methane with (x,y,z) = (4,1,0), gasoline with approximately (x,y,z) = (18,8,0), methanol......The increasing need for easy and affordable storage of intermittent renewable energy has encouraged us to explore the possibilities of pressurized electrolysis and fuel cells operating in the temperature range of 200 – 300 °C and pressure from a few bar up to 50 bar and above. Most electrochemical...... rate limiting processes are strongly thermal activated. Also, increased pressure may increase the electrode reaction rates. High pressure means increase energy density in gaseous products. Furthermore, as hydrocarbons, alcohols or ethers - in common denoted HxCyOz - are very convenient fuels, we have...

  4. Fleet Assistance and Support Team (FAST) Lab

    Data.gov (United States)

    Federal Laboratory Consortium — The FAST team was established by PMA-264 for introduction of multistatic ASW systems into the Fleet.FAST provides Air ASW mission planning, tactics/tactical sensor...

  5. Fleet replacement modeling : final report, July 2009.

    Science.gov (United States)

    2009-07-01

    This project focused on two interrelated areas in equipment replacement modeling for fleets. The first area was research-oriented and addressed a fundamental assumption in engineering economic replacement modeling that all assets providing a similar ...

  6. Temporal Optimization Planning for Fleet Repositioning

    DEFF Research Database (Denmark)

    Tierney, Kevin; Jensen, Rune Møller

    2011-01-01

    Fleet repositioning problems pose a high financial bur- den on shipping firms, but have received little attention in the literature, despite their high importance to the shipping industry. Fleet repositioning problems are characterized by chains of interacting activities, but state-of-the-art pla......Fleet repositioning problems pose a high financial bur- den on shipping firms, but have received little attention in the literature, despite their high importance to the shipping industry. Fleet repositioning problems are characterized by chains of interacting activities, but state......-of-the-art planning and scheduling techniques do not offer cost models that are rich enough to represent essential objectives of these problems. To this end, we introduce a novel framework called Temporal Optimization Planning (TOP). TOP uses partial order planning to build optimization models associated...

  7. Future Fleet Project. What Can We Afford

    Science.gov (United States)

    2016-12-21

    available to maintain the readiness of the fleet. The reduction in readiness spending coupled with continuing demands from COCOMs for assets eroded... coupled with continuing demands from COCOMs for assets eroded the number of ships in ready‑to‑deploy status. CBO’s estimate of $20.2 billion per...less-expensive aircraft carriers, and additional SSNs. Our intent in designing this fleet is to use the money saved in buying less-expensive

  8. CleanFleet. Final report: Executive summary

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-12-01

    CleanFleet, formally known as the South Coast Alternative Fuels Demonstration, was a comprehensive demonstration of alternative fuel vehicles (AFVs) in daily commercial service. Between April 1992 and September 1994, five alternative fuels were tested in 84 panel vans: compressed natural gas (CNG), propane gas, methanol as M-85, California Phase 2 reformulated gasoline (RFG), and electricity. The AFVs were used in normal FedEx package delivery service in the Los Angeles basin alongside 27 {open_quotes}control{close_quotes} vans operating on regular gasoline. The liquid and gaseous fuel vans were model year 1992 vans from Ford, Chevrolet, and Dodge. The two electric vehicles (EVs) were on loan to FedEx from Southern California Edison. The AFVs represented a snapshot in time of 1992 technologies that (1) could be used reliably in daily FedEx operations and (2) were supported by the original equipment manufacturers (OEMs). A typical van is shown in Figure 2. The objective of the project was to demonstrate and document the operational, emissions, and economic status of alternative fuel, commercial fleet delivery vans in the early 1990s for meeting air quality regulations in the mid to late 1990s. During the two-year demonstration, CleanFleet`s 111 vehicles travelled more than three million miles and provided comprehensive data on three major topics: fleet operations, emissions, and fleet economics. Fleet operations were examined in detail to uncover and resolve problems with the use of the fuels and vehicles in daily delivery service. Exhaust and evaporative emissions were measured on a subset of vans as they accumulated mileage. The California Air Resources Board (ARB) measured emissions to document the environmental benefits of these AFVs. At the same time, CleanFleet experience was used to estimate the costs to a fleet operator using AFVs to achieve the environmental benefits of reduced emissions.

  9. What is behind fleet evolution: a framework for flow analysis and application to the French Atlantic fleet

    OpenAIRE

    Quillérou, Emmanuelle; Guyader, Olivier

    2012-01-01

    The study of fishery dynamics considers national-level fleet evolution. It has, however, failed to consider the flows behind fleet evolution as well as the impact of the dynamics of owners of invested capital on fleet evolution. This paper establishes a general conceptual framework which identifies different vessel and owner flows behind fleet evolution and some relationships between these flows. This descriptive conceptual framework aims to change the current focus on drivers of fleet evolut...

  10. Fleet management tools for the utility industry

    Energy Technology Data Exchange (ETDEWEB)

    Caywood, J. [Terex Telelect, Watertown, SD (United States)

    2002-08-01

    In order to enable fleet managers to make the appropriate decisions concerning buying, selling, renting, leasing or repairing vehicles, information about ownership and operating cost and utilization are important. Fleet management tools are now available in the heavy construction industry. The author describes one such fleet management services customized for the utility industry: FleetEdge. This system utilizes current and future technologies to enable the managers to take proactive or predictive approaches to fleet management. An example of a company that has opted for this system was reviewed: Reliant Energy-Houston Metro. The data collection process was explained. A mini-computer processor is fitted on the equipment, and the requested monitoring parameters in turn determine the variety of sensors and alarms to be selected. Location can also be monitored through the use of Global Positioning System (GPS) equipment. The parameters normally include low engine oil pressure, high engine operating temperature, high transmission operating temperature, high hydraulic oil temperature, engine hours. Wireless data transfer is effected via cellular or satellite networks. The data is then collected and checked for errors and format at a hub before going to the computer server through the Internet. Information is transmitted from the accounting system to fleet management service through a secure interface. The data is then configured to meet the client's needs. It results in up to date, understandable information to the client. Equipment utilization, downtime and cost by machine or category is easily accessible and printed if required. Fleet equipment status is provided, as well as hourly cost analysis. Other reports are also available. 2 tabs., 2 figs.

  11. Articulismo en democracia. Las columnas de Antonio Muñoz Molina

    Directory of Open Access Journals (Sweden)

    Natalia Corbellini

    2008-11-01

    Full Text Available The writer's column format related to Muñoz Molina's last novels (Sefarad. Una novela de novelas; Ventanas de Manhattan; Días de diario , where the concept of genre is not clear, they alternate narrative and argumentation, tales and essay. I will analyze the writing of published columns as "Ida y vuelta" in the suplement Babelia of El país, as a format that allows experimentation with the language and the narrator; and simultaneously constructs an ego with the will of style, and for providing a rewatching form of knowledge and thought

  12. Genome-wide association study identifies novel loci associated with circulating phospho- and sphingolipid concentrations

    NARCIS (Netherlands)

    A. Demirkan (Ayşe); C.M. van Duijn (Cornelia); P. Ugocsai (Peter); A.J. Isaacs (Aaron); P.P. Pramstaller (Peter Paul); G. Liebisch (Gerhard); J.F. Wilson (James); A. Johansson (Åsa); I. Rudan (Igor); Y.S. Aulchenko (Yurii); A.V. Kirichenko (Anatoly); A.C.J.W. Janssens (Cécile); R.C. Jansen (Ritsert); C. Gnewuch (Carsten); I. Domingues (Inês); C. Pattaro (Cristian); S.H. Wild (Sarah); I. Jonasson (Inger); O. Polasek (Ozren); I.V. Zorkoltseva (Irina); A. Hofman (Albert); L.C. Karssen (Lennart); M.V. Struchalin (Maksim); J. Floyd (Jamie); W. Igl (Wilmar); Z. Biloglav (Zrinka); L. Broer (Linda); A. Pfeufer (Arne); I. Pichler (Irene); S. Campbell (Susan); G. Zaboli (Ghazal); I. Kolcic (Ivana); F. Rivadeneira Ramirez (Fernando); J.E. Huffman (Jennifer); N. Hastie (Nick); A.G. Uitterlinden (André); L. Franke (Lude); C.S. Franklin (Christopher); V. Vitart (Veronique); C.P. Nelson (Christopher P.); M. Preuss (Michael); J.C. Bis (Joshua); C.J. O'Donnell (Christopher); N. Franceschini (Nora); J.C.M. Witteman (Jacqueline); T.I. Axenovich (Tatiana); B.A. Oostra (Ben); T. Meitinger (Thomas); A.A. Hicks (Andrew); C. Hayward (Caroline); A.F. Wright (Alan); U. Gyllensten (Ulf); H. Campbell (Harry); G. Schmitz (Gerd)

    2012-01-01

    textabstractPhospho- and sphingolipids are crucial cellular and intracellular compounds. These lipids are required for active transport, a number of enzymatic processes, membrane formation, and cell signalling. Disruption of their metabolism leads to several diseases, with diverse neurological,

  13. Phospho.ELM: a database of phosphorylation sites--update 2011

    DEFF Research Database (Denmark)

    Dinkel, Holger; Chica, Claudia; Via, Allegra

    2011-01-01

    The Phospho.ELM resource (http://phospho.elm.eu.org) is a relational database designed to store in vivo and in vitro phosphorylation data extracted from the scientific literature and phosphoproteomic analyses. The resource has been actively developed for more than 7 years and currently comprises ...... sequence alignment used for the score calculation. Finally, special emphasis has been put on linking to external resources such as interaction networks and other databases.......The Phospho.ELM resource (http://phospho.elm.eu.org) is a relational database designed to store in vivo and in vitro phosphorylation data extracted from the scientific literature and phosphoproteomic analyses. The resource has been actively developed for more than 7 years and currently comprises 42...

  14. Influence of a Phospho-Potassic fertilizer solution on yield and quality of Wheat Crops

    Energy Technology Data Exchange (ETDEWEB)

    Ordonez, C.; Tejada, M.; Gonzalez, J. L.; Benitez, C.

    2009-07-01

    There is currently interest in the use of industrial by-products to reduce the use of synthetic fertilizers. For this reason, in this paper the influence of a phospho-potassic fertilizer solution obtained from a aminoacid production process on wheat crops is studied. The positive influence on leaf potassium contents was most significant when the dosage of phospho-potassic fertilizer solution was applied to bread wheat. (Author)

  15. Fleet Tools; NREL (National Renewable Energy Laboratory)

    Energy Technology Data Exchange (ETDEWEB)

    None

    2015-04-01

    From beverage distributors to shipping companies and federal agencies, industry leaders turn to the National Renewable Energy Laboratory (NREL) to help green their fleet operations. Cost, efficiency, and reliability are top priorities for fleets, and NREL partners know the lab’s portfolio of tools can pinpoint fuel efficiency and emissions-reduction strategies that also support operational the bottom line. NREL is one of the nation’s foremost leaders in medium- and heavy-duty vehicle research and development (R&D) and the go-to source for credible, validated transportation data. NREL developers have drawn on this expertise to create tools grounded in the real-world experiences of commercial and government fleets. Operators can use this comprehensive set of technology- and fuel-neutral tools to explore and analyze equipment and practices, energy-saving strategies, and other operational variables to ensure meaningful performance, financial, and environmental benefits.

  16. Structure-Activity Relationship of the Antimalarial Ozonide Artefenomel (OZ439).

    Science.gov (United States)

    Dong, Yuxiang; Wang, Xiaofang; Kamaraj, Sriraghavan; Bulbule, Vivek J; Chiu, Francis C K; Chollet, Jacques; Dhanasekaran, Manickam; Hein, Christopher D; Papastogiannidis, Petros; Morizzi, Julia; Shackleford, David M; Barker, Helena; Ryan, Eileen; Scheurer, Christian; Tang, Yuanqing; Zhao, Qingjie; Zhou, Lin; White, Karen L; Urwyler, Heinrich; Charman, William N; Matile, Hugues; Wittlin, Sergio; Charman, Susan A; Vennerstrom, Jonathan L

    2017-04-13

    Building on insights gained from the discovery of the antimalarial ozonide arterolane (OZ277), we now describe the structure-activity relationship (SAR) of the antimalarial ozonide artefenomel (OZ439). Primary and secondary amino ozonides had higher metabolic stabilities than tertiary amino ozonides, consistent with their higher pK a and lower log D 7.4 values. For primary amino ozonides, addition of polar functional groups decreased in vivo antimalarial efficacy. For secondary amino ozonides, additional functional groups had variable effects on metabolic stability and efficacy, but the most effective members of this series also had the highest log D 7.4 values. For tertiary amino ozonides, addition of polar functional groups with H-bond donors increased metabolic stability but decreased in vivo antimalarial efficacy. Primary and tertiary amino ozonides with cycloalkyl and heterocycle substructures were superior to their acyclic counterparts. The high curative efficacy of these ozonides was most often associated with high and prolonged plasma exposure, but exposure on its own did not explain the presence or absence of either curative efficacy or in vivo toxicity.

  17. Fleet wide motor asset management program

    International Nuclear Information System (INIS)

    Rosemeier, R.G.; Moxie, J.J.; Mendez, W.E.

    2011-01-01

    The Institute of Nuclear Power Operations (INPO) performed several studies concerning the effects of motor failures on US power production. Problems with aging and the lack of preventative maintenance were noted as major contributors to this production loss. Westinghouse has developed, and successfully utilized, a computer program to assist plants in the decision making process for maximizing the operational availability of their motor fleet. The program considers motors of a fleet as a single, critical to production asset, and aids the operator in decisions regarding the following: 1.) Purchase of spares. 2.) Rewind and refurbishment prioritization. 3.) Long-term budget forecasting. (author)

  18. Clean Cities Case Study: Barwood Cab Fleet Study Summary

    International Nuclear Information System (INIS)

    Whalen, P.

    1999-01-01

    Barwood Cab Fleet Study Summary is the second in a new series called''Alternative Fuel Information Case Studies,'' designed to present real-world experiences with alternative fuels to fleet managers and other industry stakeholders

  19. Fleet equipment performance measurement preventive maintenance model : final report.

    Science.gov (United States)

    2014-04-01

    The concept of preventive maintenance is very important in the effective management and deployment of : vehicle fleets. The Texas Department of Transportation (TxDOT) operates a large fleet of on-road and offroad : equipment. Newer engines and vehicl...

  20. Intravesicular Phosphatase PHOSPHO1 Function in Enamel Mineralization and Prism Formation

    Directory of Open Access Journals (Sweden)

    Mirali Pandya

    2017-10-01

    Full Text Available The transport of mineral ions from the enamel organ-associated blood vessels to the developing enamel crystals involves complex cargo packaging and carriage mechanisms across several cell layers, including the ameloblast layer and the stratum intermedium. Previous studies have established PHOSPHO1 as a matrix vesicle membrane-associated phosphatase that interacts with matrix vesicles molecules phosphoethanolamine and phosphocholine to initiate apatite crystal formation inside of matrix vesicles in bone. In the present study, we sought to determine the function of Phospho1 during amelogenesis. PHOSPHO1 protein localization during amelogenesis was verified using immunohistochemistry, with positive signals in the enamel layer, ameloblast Tomes' processes, and in the walls of ameloblast secretory vesicles. These ameloblast secretory vesicle walls were also labeled for amelogenin and the exosomal protein marker HSP70 using immunohistochemistry. Furthermore, PHOSPHO1 presence in the enamel organ was confirmed by Western blot. Phospho1−/− mice lacked sharp incisal tips, featured a significant 25% increase in total enamel volume, and demonstrated a significant 2-fold reduction in silver grain density of von Kossa stained ground sections indicative of reduced mineralization in the enamel layer when compared to wild-type mice (p < 0.001. Scanning electron micrographs of Phospho1−/− mouse enamel revealed a loss of the prominent enamel prism “picket fence” structure, a loss of parallel crystal organization within prisms, and a 1.56-fold increase in enamel prism width (p < 0.0001. Finally, EDS elemental analysis demonstrated a significant decrease in phosphate incorporation in the enamel layer when compared to controls (p < 0.05. Together, these data establish that the matrix vesicle membrane-associated phosphatase PHOSPHO1 is essential for physiological enamel mineralization. Our findings also suggest that intracellular ameloblast secretory

  1. 48 CFR 970.2307-1 - Motor vehicle fleet operations.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Motor vehicle fleet..., Renewable Energy Technologies, Occupational Safety and Drug-Free Work Place 970.2307-1 Motor vehicle fleet... that the Federal motor vehicle fleet will serve as an example and provide a leadership role in the...

  2. Transportation capabilities of the existing cask fleet

    International Nuclear Information System (INIS)

    Johnson, P.E.; Joy, D.S.; Wankerl, M.W.

    1991-01-01

    This paper describes a number of scenarios estimating the amount of spent nuclear fuel that could be transported to a Monitored Retrievable Storage (MRS) Facility by various combinations of existing cask fleets. To develop the scenarios, the data provided by the Transportation System Data Base (TSDB) were modified to reflect the additional time for cask turnaround resulting from various startup and transportation issues. With these more realistic speed and cask-handling assumptions, the annual transportation capability of a fleet consisting of all of the existing casks is approximately 46 metric tons of uranium (MTU). The most likely fleet of existing casks that would be made available to the Department of Energy (DOE) consists of two rail, three overweight truck, and six legal weight truck casks. Under the same transportation assumptions, this cask fleet is capable of approximately transporting 270 MTU/year. These ranges of capability is a result of the assumptions pertaining to the number of casks assumed to be available. It should be noted that this assessment assumes additional casks based on existing certifications are not fabricated. 5 refs., 4 tabs

  3. Strategic fleet planning for city logistics

    NARCIS (Netherlands)

    Franceschetti, Anna; Honhon, Dorothee; Laporte, G.; van Woensel, T.; Fransoo, J.C.

    2017-01-01

    We study the strategic problem of a logistics service provider managing a (possibly heterogeneous) fleet of vehicles to serve a city in the presence of access restrictions. We model the problem as an area partitioning problem in which a rectangular service area has to be divided into sectors, each

  4. Transportation capabilities of the existing cask fleet

    International Nuclear Information System (INIS)

    Johnson, P.E.; Wankerl, M.W.; Joy, D.S.

    1991-01-01

    This paper describes a number of scenarios estimating the amount of spent nuclear fuel that could be transported to a Monitored Retrievable Storage (MRS) Facility by various combinations of existing cask fleets. To develop the scenarios, the data provided by the Transportation System Data Base (TSDB) were modified to reflect the additional time for cask turnaround resulting from various startup and transportation issues. With these more realistic speed and cask-handling assumptions, the annual transportation capability of a fleet consisting of all of the existing casks is approximately 465 metric tons of uranium (MTU). The most likely fleet of existing casks that would be made available to the DOE consists of two rail, three overweight truck, and six legal weight truck casks. Under the same transportation assumptions, this cask fleet is capable of approximately transporting 270 MTU/year. These ranges of capability is a result of the assumptions pertaining to the number of casks assumed to be available. It should be noted that this assessment assumes additional casks based on existing certifications are not fabricated

  5. Formation of optimal construction fleet composition

    Science.gov (United States)

    Tuskaeva, Zalina

    2017-10-01

    Machinery supply and its rational use in construction processes considerably determine the final product of construction organizations. Therefore, the problem of defining the type size composition of the construction fleet as one of the lowest material-intensive productions, is of a particular importance.

  6. Implementing Automotive Telematics for Fleet Insurance

    Directory of Open Access Journals (Sweden)

    Marika Azzopardi

    2013-12-01

    Full Text Available The advantages of Usage-Based Insurance for automotive covers over conventional rating methods have been discussed in literature for over four decades. Notwithstanding their adoption in insurance markets has been slow. This paper seeks to establish the viability of introducing fleet Telematics-Based Insurance by investigating the perceptions of insurance operators, tracking service providers and corporate fleet owners. At its core, the study involves a SWOT-analysis to appraise Telematics-Based Insurance against conventional premium rating systems. Twenty five key stakeholders in Malta, a country with an insurance industry that represents others in microcosm, were interviewed to develop our analysis. We assert that local insurers have interests in such insurance schemes as enhanced fleet management and monitoring translate into an improved insurance risk. The findings presented here have implications for all stakeholders as we argue that telematics enhance fleet management, TBI improves risk management for insurers and adoption of this technology is dependent on telematics providers increasing the perceived control by insurers over managing this technology.

  7. TEMAS: fleet-based bio-economic simulation software to evaluate management strategies accounting for fleet behaviour

    DEFF Research Database (Denmark)

    Ulrich, Clara; Andersen, Bo Sølgaard; Sparre, Per Johan

    2007-01-01

    TEMAS (technical management measures) is a fleet-based bio-economic software for evaluating management strategies accounting for technical measures and fleet behaviour. It focuses on mixed fisheries in which several fleets can choose among several fishing activities to target different stocks...

  8. PhosphoRice: a meta-predictor of rice-specific phosphorylation sites

    Directory of Open Access Journals (Sweden)

    Que Shufu

    2012-02-01

    Full Text Available Abstract Background As a result of the growing body of protein phosphorylation sites data, the number of phosphoprotein databases is constantly increasing, and dozens of tools are available for predicting protein phosphorylation sites to achieve fast automatic results. However, none of the existing tools has been developed to predict protein phosphorylation sites in rice. Results In this paper, the phosphorylation site predictors, NetPhos 2.0, NetPhosK, Kinasephos, Scansite, Disphos and Predphosphos, were integrated to construct meta-predictors of rice-specific phosphorylation sites using several methods, including unweighted voting, unreduced weighted voting, reduced unweighted voting and weighted voting strategies. PhosphoRice, the meta-predictor produced by using weighted voting strategy with parameters selected by restricted grid search and conditional random search, performed the best at predicting phosphorylation sites in rice. Its Matthew's Correlation Coefficient (MCC and Accuracy (ACC reached to 0.474 and 73.8%, respectively. Compared to the best individual element predictor (Disphos_default, PhosphoRice archieved a significant increase in MCC of 0.071 (P Conclusions PhosphoRice is a powerful tool for predicting unidentified phosphorylation sites in rice. Compared to the existing methods, we found that our tool showed greater robustness in ACC and MCC. PhosphoRice is available to the public at http://bioinformatics.fafu.edu.cn/PhosphoRice.

  9. Application of Strategic Planning Process with Fleet Level Analysis Methods

    Science.gov (United States)

    Mavris, Dimitri N.; Pfaender, Holger; Jimenez, Hernando; Garcia, Elena; Feron, Eric; Bernardo, Jose

    2016-01-01

    The goal of this work is to quantify and characterize the potential system-wide reduction of fuel consumption and corresponding CO2 emissions, resulting from the introduction of N+2 aircraft technologies and concepts into the fleet. Although NASA goals for this timeframe are referenced against a large twin aisle aircraft we consider their application across all vehicle classes of the commercial aircraft fleet, from regional jets to very large aircraft. In this work the authors describe and discuss the formulation and implementation of the fleet assessment by addressing the main analytical components: forecasting, operations allocation, fleet retirement, fleet replacement, and environmental performance modeling.

  10. Synthesis and electrical properties of (Pb,Co)Sr2(Y,Ca)Cu2Oz

    Science.gov (United States)

    Tashiro, T.; Maeda, T.; Abe, R.; Takechi, S.; Takahashi, T.; Haruta, M.; Horii, S.

    One of related materials to high-temperature superconductors (HTSC's) with nominal compositions of (Pb0.5Co0.5)Sr2(Y1xCax)Cu2Oz (x=0∼0.6) is synthesized and characterized. All samples are nearly single-phase, and its crystal structure is likely to be so-called "1-2-1-2" type which is one of typical structures of HTSC's. Electrical resistivity is decreased as x increases. While superconductivity is not observed at temperatures between room-temperature and 20 K for all samples, temperature dependence of the resistivity exhibits metallic behavior down to 150 K for x=0.5. Phase formation and transport behavior are discussed focusing on mixed valence-state of Co2+ and Co3+.

  11. The Idea of Science of Brazilian Physiologist Miguel Ozório de Almeida (1890-1953

    Directory of Open Access Journals (Sweden)

    Letícia Pumar

    2017-12-01

    Full Text Available Epistemological considerations of philosophers and scientists from the late nineteenth century to the mid-twentieth century guided Brazilian physiologist Miguel Ozório de Almeida (1890-1953 in formulating his researches and participating in national and international scientific debates. With his siblings, Álvaro Ozório de Almeida and Branca de Almeida Fialho, he participated in debates on Brazilian educational and scientific system’s reform and in international organizations. The family’s residence in Rio de Janeiro housed a laboratory that became a reference in experimental physiology researches in Brazil. This article aims to present Miguel Ozório de Almeida’s conception of science, constructed mainly within the private laboratory’s sociability, providing new aspects of scientific work production in Brazil in the early twentieth century. I argue that Ozório de Almeida’s stand as an internationalist physiologist in national and international contexts was related to his reading of texts by Ernest Mach, Pierre Duhem, Henri Poincaré and William James.

  12. BeerOz, a set of Matlab routines for the quantitative interpretation of spectrophotometric measurements of metal speciation in solution

    Science.gov (United States)

    Brugger, Joël

    2007-02-01

    The modelling of the speciation and mobility of metals under surface and hydrothermal conditions relies on the availability of accurate thermodynamic properties for all relevant minerals, aqueous species, gases and surface species. Spectroscopic techniques obeying the Beer-Lambert law can be used to obtain thermodynamic properties for reactions among aqueous species (e.g., ligand substitution; protonation). BeerOz is a set of Matlab routines designed to perform both qualitative and quantitative analysis of spectroscopic data following the Beer-Lambert law. BeerOz is modular and can be customised for particular experimental strategies or for simultaneous refinement of several datasets obtained using different techniques. Distribution of species calculations are performed using an implementation of the EQBRM code, which allows for customised activity coefficient calculations. BeerOz also contains routines to study the n-dimensional solution space, in order to provide realistic estimates of errors and test for the existence of multiple local minima and correlation between the different refined variables. The paper reviews the physical principles underlying the qualitative and quantitative analysis of spectroscopic data collected on aqueous speciation, in particular for studying successive ligand replacement reactions, and presents the non-linear least-squares algorithm implemented in BeerOz. The discussion is illustrated using UV-Vis spectra collected on acidic Fe(III) solutions containing varying LiCl concentrations, and showing the change from the hexaaquo Fe(H 2O) 63+ complex to the tetrahedral FeCl 4- complex.

  13. Fleet operator risks for using fleets for V2G regulation

    International Nuclear Information System (INIS)

    Hill, Davion M.; Agarwal, Arun S.; Ayello, Francois

    2012-01-01

    Future fleets of vehicles may include electric vehicles (EVs) or hybrid electric vehicles (HEVs) because of potential fuel savings. Recent demonstration of diesel parallel hybrids in a delivery fleet led to fuel economy improvements, and hybrid bus demonstrations exhibited twice the fuel economy of the conventional bus. Fleet ownership may include management of a fleet of vehicles as small as 10 units and as large as hundreds or thousands. In addition to fuel savings, the newer extended range electric vehicles (EREVs) and pure EVs permit vehicle to grid (V2G) opportunities. These V2G opportunities may present additional revenue for fleets by providing ancillary services to local grid independent system operators (ISOs), provided that the burden of driving and V2G services do not accelerate the degradation of the battery systems in these vehicles. The subject of this study is to determine the financial risks associated with accelerated battery degradation in a V2G-enabled EREV fleet expected to perform ancillary service duty while charging in addition to the normal loads of drive cycle duty. We determine that battery cycle life during V2G duty is a critical parameter, which can determine whether or not the business model is viable. - Highlights: ► V2G regulation cycle life of EREV batteries must be >50,000 cycles to be profitable. ► Present knowledge and test data about the impact of V2G cycles on battery life are limited. ► Replacement of batteries and energy throughput are major factors in the cost–benefit analysis. ► V2G fleet is not viable with present data but can be viable with some technical advancement.

  14. Policies towards a more efficient car fleet

    International Nuclear Information System (INIS)

    Mandell, Svante

    2009-01-01

    Transportation within the EU, as in most of the industrialized world, shows an increasing trend in CO 2 emissions. This calls for measures to decrease the amount of transportation but also to increase the efficiency in the vehicle fleet. To achieve this, numerous policy measures are available, all of which targets the agents in the economy in various ways. Policy makers thus face a highly complex task. The present paper aims at providing a simple and transparent analytical model that illustrates how different policy measures address different parts of an interlinked system, which determines the composition of the future car fleet. Apart from being simple, and thereby providing an intuitive framework, the model provides important lessons for policy design, e.g., through highlighting the difference between initial responses to policies and the outcome in equilibrium both in the short and the long run.

  15. Advanced technology mobile robotics vehicle fleet

    International Nuclear Information System (INIS)

    McGovern, D.E.

    1987-03-01

    A fleet of vehicles is being developed and maintained by Sandia National Laboratories for studies in remote control and autonomous operation. The vehicles range from modified commercial vehicles to specially constructed mobile platforms and are utilized as testbeds for developing concepts in the areas of remote control (teleoperation) and computer control (autonomy). Actuators control the vehicle speed, brakes, and steering via manual input from a remote driving station or through some level of digital computer control. On-board processing may include simple vehicle control functions or may allow for unmanned, autonomous operation. Communication links are provided for digital communication between control computers, television transmission for vehicle vision, and voice for local control. SNL can develop, test, and evaluate sensors, processing requirements, various methods of actuator implementation, operator controlled feedback requirements, and vehicle operations. A description of the major features and uses for each of the vehicles in the fleet is provided

  16. DESIGNING OF AN AUTOMOBILE FLEET NETWORK

    Directory of Open Access Journals (Sweden)

    R. B. Ivut

    2016-01-01

    Full Text Available Investment volume is considered as an important factor in regional development under current conditions. Logistical infrastructure which ensures a complex transport, distributive, information and other services exerts a significant influence on regional investment attractiveness. Lack of clear vision on development and execution of development strategy for logistics infrastructure from the side of regional authorities results in unwillingness of large federal and transnational companies to provide investments in infrastructure projects. Network of automotive transport terminals is one of the main elements in logistics infrastructure. The network allows to optimize a flow of material goods from the point of their origin to the point of their consumption with the lowest possible costs and the required level of service. Automobile transport is one of the main objects of transport infrastructure and it is characterized by rather high flexibility in comparison with other types of transport facilities that preconditions its widespread application. Network of automobile fleets (terminals has been formed for redistribution of goods traffic within the concerned regions. The purpose of the present research is to develop a mathematical model for formation of transport infrastructure on the territory of regions. The paper proposes an approach for formation of automobile fleet (terminal network on the territory of a large region with due account of the established network of distribution and sorting-out warehouse facilities. A model has been developed for solving the problem pertaining to minimization of aggregate costs related to maintenance of automobile fleets, delivery of goods to and from distribution and sorting-out warehouse facilities to consumers, ferry of empty trucks and goods handling. The model makes it possible to determine optimal number and location area of automobile fleets (terminals while accounting for their possible locations, capacity

  17. Mario Muñoz, el médico del Moncada

    Directory of Open Access Journals (Sweden)

    Natalia I. Osorio Curbelo

    2015-11-01

    Full Text Available Nació en Colón, Matanzas, el 26 de julio de 1912, hijo de Marcelino Muñoz Zurra, natural de Roque, Colón, de profesión fotógrafo y de Catalina Monroy Artiles, natural de Colon. Estudió la primera enseñanza y la secundaria en el Instituto de Colón. Obtuvo el Título de Bachiller en Ciencias y Letras a los 21 años, en el Instituto de Segunda Enseñanza de La Habana, el 28 de agosto de 1934. Ingresó en la Universidad de La Habana, en el curso académico de 1934-1935, donde matriculó la carrera de Medicina. En ese momento existía una gran crisis política, económica y social en Cuba, pues el estudiantado se encontraba en franca rebeldía contra el tirano Machado, y Mario Muñoz Monroy ingresó en el directorio estudiantil universitario. Fue uno de los líderes de la histórica huelga de marzo de 1935. El 5 de febrero 1942 logró por fin habiendo regresado a las aulas, hacer su ejercicio de grado, obteniendo nota de sobresaliente, graduándose el 16 de marzo. Vestía bata blanca y trataba con humildad a sus semejantes. Algunos le criticaban, porque cuando el paciente le necesitaba no le preguntaba si tenía dinero para pagar, le atendía sin miramientos... En la década de los años 50 del siglo XX, Mario Muñoz Monroy, en la ciudad de Colón, provincia de Matanzas, estaba al tanto de las noticias, sabía que el futuro de la Patria no podría ser el de golpes de estado, represión, tiranía e inconstitucionalidad. Antes combatió a Gerardo Machado, porque amaba la justicia, era hombre cabal, por eso no dudó en enrolarse en el movimiento que pasó a la historia como la Generación del Centenario. De su casa de la calle Diago, hoy Museo Casa de los Mártires del Moncada, en Colón, partió en su auto hacia Santiago de Cuba para encontrarse con la historia. (1 Cumplía 41 años de edad, y es conocido su diálogo con Fidel Castro, líder del grupo de jóvenes martianos y revolucionarios que asaltaron la madrugada en aquel julio caluroso y

  18. Cesium-137 uptake studies on ammonium phospho molybdate irradiated with electrons

    International Nuclear Information System (INIS)

    Rao, K.L.N.; Balasubramanian, K.R.; Shukla, J.P.

    1992-01-01

    Ammonium phospho molybdate is an important inorganic ion exchanger having high selectivity for cesium. This paper discusses the effects of electron irradiation to a dose of 1 mGy on this exchanger with special reference to its ion exchange performance using cesium-137 as a tracer. An explanation is attempted for the slight increase in the distribution coefficients. (author). 5 refs., 1 tab

  19. Focused microwave irradiation-assisted immunohistochemistry to study effects of ketamine on phospho-ERK expression in the mouse brain.

    Science.gov (United States)

    Fernandes, Alda; Li, Yu-Wen

    2017-09-01

    Ketamine produces rapid and long-lasting antidepressant effects in depressive patients. Preclinical studies demonstrate that ketamine stimulates AMPA receptor transmission and activates BDNF/TrkB-Akt/ERK-mTOR signaling cascades, leading to a sustained increase in synaptic protein synthesis and strengthening of synaptic plasticity, a potential mechanism underlying the antidepressant effects. The purpose of this study was to develop an immunohistochemistry (IHC) assay to map the distribution of extracellular signal-regulated kinase (ERK) phosphorylation in the mouse brain in response to systemic ketamine treatment. We established a focused microwave irradiation-assisted IHC assay to detect phosphorylated (phospho) proteins including phospho-ERK, phospho- cAMP-response- element-binding protein (CREB), phospho- glutamate receptor 1 (GluR1) and phospho- calcium/calmodulin-dependent protein kinase II (CaMKII) with greater sensitivity and reproducibility in comparison to conventional IHC methods. A single dose of ketamine produced a robust, dose- and time-dependent increase in phospho-ERK immunoreactive (phospho-ERK-ir) neurons in the medial prefrontal cortex (mPFC) and the central nucleus of the amygdala. Phospho-ERK-ir neurons in the mPFC were primarily located in the prelimbic and anterior cingulate subregions with the morphology resembling pyramidal neurons. An increase in phospho-ERK-ir was also observed in the brainstem dorsal raphe nucleus and locus coeruleus. The NMDA GluN2B subtype receptor antagonist Ro 25-6981 increased phospho-ERK expression in the brain in a similar pattern as ketamine. In summary, we have established a sensitive and reliable focused microwave irradiation-assisted IHC assay, and defined the activation pattern of ERK, in response to systemic ketamine and Ro 25-6981 treatment, in brain regions that are potentially responsible for mediating the antidepressant effects. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Excellence in fleet combat replacement squadrons: predicting carrier qualification success

    OpenAIRE

    Smith, Martin P.

    1988-01-01

    This thesis presents a two-part analysis of excellence criteria for fleet combat replacement squadrons. Part one focuses on the qualitative issues and management techniques identified in outstanding fleet combat replacement squadrons. Part two develops and presents a regression model for predicting a fleet replacement squadron pilot's carrier qualification grade. The model was derived using standard linear regression techniques and the SPSSx software package of the Naval Postgraduate School. ...

  1. Size and transportation capabilities of the existing US cask fleet

    International Nuclear Information System (INIS)

    Danese, F.L.; Johnson, P.E.; Joy, D.S.

    1990-01-01

    This study investigates the current spent nuclear fuel cask fleet capability in the United States. In addition, it assesses the degree to which the current fleet would be available, as a contingency, until proposed Office of Civilian Radioactive Waste Management casks become operational. A limited fleet of ten spent fuel transportation casks is found to be readily available for use in Federal waste management efforts over the next decade

  2. 41 CFR 102-34.330 - What is the Federal Fleet Report?

    Science.gov (United States)

    2010-07-01

    ... Fleet Report? 102-34.330 Section 102-34.330 Public Contracts and Property Management Federal Property... MANAGEMENT Federal Fleet Report § 102-34.330 What is the Federal Fleet Report? The Federal Fleet Report (FFR..., in evaluating the effectiveness of the operation and management of individual fleets to determine...

  3. Radioactive Legacy of the Russian Pacific Fleet Operations. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Compton, K. L.; Novikov, V.M.; Parker, F.L.; Sivintsev, Y.U.

    2003-03-25

    There have been extensive studies of the current and potential environmental impact of Russian Northern fleet activities. However, despite the fact that the total number of ships in both fleets are comparable, there have been very few studies published in the open literature of the impact of the Pacific fleet. This study of the Pacific fleet's impact on neighboring countries was undertaken to partially remedy this lack of analysis. This study is focused on an evaluation of the inventory of major sources of radioactive material associated with the decommissioning of nuclear submarines, and an evaluation of releases to the atmosphere and their long-range (>100km) transboundary transport.

  4. Research on Operational Aspects of Large Autonomous Underwater Glider Fleets

    National Research Council Canada - National Science Library

    Fratantoni, David M

    2007-01-01

    This program supported research on the operational and management issues stemming from application of large fleets of autonomous underwater gliders to oceanographic research and rapid environmental...

  5. Large Fleets Lead in Petroleum Reduction (Fact Sheet)

    Energy Technology Data Exchange (ETDEWEB)

    Proc, H.

    2011-03-01

    Fact sheet describes Clean Cities' National Petroleum Reduction Partnership, an initiative through which large private fleets can receive support from Clean Cities to reduce petroleum consumption.

  6. High temperature dielectric properties of (BxNyOz thin films deposited using ion source assisted physical vapor deposition

    Directory of Open Access Journals (Sweden)

    N. Badi

    2015-12-01

    Full Text Available The dielectric integrity has been one of the major obstacle in bringing out capacitor devices with suitable performance characteristics at high temperatures. In this paper, BxNyOz dielectric films for high temperature capacitors solutions are investigated. The films were grown on silicon substrate by using ion source assisted physical vapor deposition technique. The as-grown films were characterized by SEM, XRD, and XPS. The capacitor structures were fabricated using BxNyOz as a dielectric and titanium as metal electrodes. The elaborated devices were subjected to electrical and thermal characterization. They exhibited low electrical loss and very good stability when subjected to high temperature for a prolonged period of time.

  7. " Judas ," Amos Oz : A Thinking of Law Review and Show Thought

    Directory of Open Access Journals (Sweden)

    Jesualdo Eduardo de Almeida Junior

    2016-05-01

    Full Text Available Este documento analiza el libro “Judas”, el escritor judío Amos Oz y su relación con el derecho a pensar y a manifestar según sus convicciones, aunque refujam a nivel tolera- ble para los conceptos previamente impuesta por la sociedad.  No hay ninguna manera de limitar el derecho a pensar, dado que este tallos desde la propia intimidad y no está sujeto a restriçõees de cualquier naturaleza, sobre todo porque no hay manera, en la práctica, limitan. La libertad de expresión de pensamiento, cuando se ejerce en su plenitud, a me- nudo sorprendente teniendo en cuenta que se enfrenta con pétreos normas impuestas por la masa. Sin embargo, todavía libertad no puede reducirse al azar por el mero hecho de una expresión impopular. Las limitaciones se presentan, sin embargo, cuando incitadoras de odio.

  8. Thermodynamics of protein folding using a modified Wako-Saitô-Muñoz-Eaton model.

    Science.gov (United States)

    Tsai, Min-Yeh; Yuan, Jian-Min; Teranishi, Yoshiaki; Lin, Sheng Hsien

    2012-09-01

    Herein, we propose a modified version of the Wako-Saitô-Muñoz-Eaton (WSME) model. The proposed model introduces an empirical temperature parameter for the hypothetical structural units (i.e., foldons) in proteins to include site-dependent thermodynamic behavior. The thermodynamics for both our proposed model and the original WSME model were investigated. For a system with beta-hairpin topology, a mathematical treatment (contact-pair treatment) to facilitate the calculation of its partition function was developed. The results show that the proposed model provides better insight into the site-dependent thermodynamic behavior of the system, compared with the original WSME model. From this site-dependent point of view, the relationship between probe-dependent experimental results and model's thermodynamic predictions can be explained. The model allows for suggesting a general principle to identify foldon behavior. We also find that the backbone hydrogen bonds may play a role of structural constraints in modulating the cooperative system. Thus, our study may contribute to the understanding of the fundamental principles for the thermodynamics of protein folding.

  9. Syntactically Silent Subjects: Luis Muñoz and the Poetry of Ellipsis

    Directory of Open Access Journals (Sweden)

    Judith Nantell

    2012-06-01

    Full Text Available Luis Muñoz (1966 is one of contemporary Spain’s most salient poets. His work has been described as demonstrating a discourse of ellipsis; yet no study has examined in detail his masterful use of syntactic and figurative omission. In fact, even though Muñoz’s published collections to date span two centuries, no single study has been devoted to his decidedly innovative expressivity. His work has been commented on in various panoramic essays considering contemporary poetry published in Spain at this temporal intersection and a number of his poems have been gathered into noteworthy anthologies of this same era. His poetry has been nominated for prestigious awards in Spain and in 2001 Correspondencias ‘Correspondences’ received the fourth Premio Internacional de Poesía del 27 as well as the Premio de Ojo Crítico de Poesía . The present study examines Muñoz’s most recent Querido silencio (2006 ‘Dear Silence’ where ellipsis figures prominently as the poet maximizes and exploits conceptual and linguistic efficiency. In one exemplary poem, “Dejar la poesía” ‘Leave Poetry,’ two syntactically silent subjects engage in an innovative conversation concerning the nature of poetry. At the same time this intriguing dialogue also offers a unique response to the essential question framing this poem and the collection as a whole: Why is poetry an addiction?

  10. Memorial and biographical history of Spain: El jinete polaco by Antonio Muñoz Molina

    Directory of Open Access Journals (Sweden)

    Felipe Oliver Fuentes Kraffczyk

    2012-12-01

    Full Text Available The novel El jinete polaco by Antonio Muñoz Molina is a highly complex work in which the narrator mixes multiple plotlines in an effort to recreate or remember part of his family past. In the process, the collective memory of the town and people of Mágina (an allegoric space that represents the peasant life of the olive growers of southern Spain appears as a rich and contradictory montage of dreams and failures. And in a third level of discourse, the recent past of Spain also emerges through the main historic al events: the civil war, the protracted military government, and the economic development and current consolidation of democracy. In order to achieve this complex and ambitious narrative project, in El jinete polaco time is not represented as uninterrupted historical timeline but as the rich mixture of the simultaneous experiences of different life courses in terms of both values and cultural patterns in order to understand how the rapid social change affects relations between the various age groups. In other words, time is constructed as a synthesis of the biographical dimension within collective memory and major historical events. This paper seeks to point out the similarities and differences between biographical discourse, collective memory and history.

  11. Retrofitting bus fleet for natural gas operation

    International Nuclear Information System (INIS)

    Stella, E.; Foresti, P.

    1992-01-01

    Buses, operating within a Florence (Italy) municipal transportation system, and equipped with Otto cycle engines, were selected for retrofitting taking into account the suitability of each vehicle's specific routing and service requirements. Cost benefit analyses indicated that it wouldn't be economically feasible to retrofit buses equipped with diesel engines. A computerized refuelling system was set up at the fleet's central service station which was hooked up to the natural gas utility's supply line. This paper tables the cost benefit analysis data comparing gasoline and methane operation and reflecting the cost savings which are expected to be accrued through this methanization program over a span of 14 years

  12. Natural gas: Fuel for urban fleets

    International Nuclear Information System (INIS)

    Mariani, F.

    1992-01-01

    The search for new ecological solutions for public transport has given an important role to natural gas for vehicles in the national context. Under current prices of fuel and costs of plants, the management of a bus fleet running on natural gas allows consistent savings, besides reducing the atmospheric pollution of urban centres. Within this context, solutions offered by current technology available on the market are examined. Low polluting emissions are taken into consideration and a complete analysis of costs and savings is reported. Reference is made to the Thermie European programme which calls for fuel diversification, energy conservation and air pollution abatement

  13. Expression and prognostic relevance of MET and phospho-BAD in non-small cell lung cancer.

    Science.gov (United States)

    Sun, Wenze; Ai, Ting; Gao, Ying; Zhang, Yingbing; Cui, Jie; Song, Liping

    2013-01-01

    MET is involved in the progression of several types of human cancers, while phospho-BAD(Ser-136) is a key molecule in apoptosis and might be regulated by MET. The aim of this study was to investigate the correlation between altered expression of MET and phospho-BAD in non-small cell lung cancer (NSCLC) and their association with clinicopathologic parameters and overall survival. MET and phospho-BAD(Ser-136) proteins were evaluated by immunohistochemical analysis in 183 paraffin-embedded specimens and were also assessed by Western blotting analysis in 12 frozen tumor tissue samples, which were representative examples of immunohistochemical staining. Positive expression of MET and phospho-BAD(Ser-136) occurred in 67.2% and 49.2% of the 183 cases of NSCLC, respectively. However, neither MET expression nor phospho-BAD(Ser-136) expression was associated with any clinicopathologic parameter. A significant correlation was found between MET and phospho-BAD(Ser-136) expression levels evaluated by immunohistochemistry (r = 0.268, P BAD(Ser-136)+ phenotype had a poorer prognosis than others (P BAD(Ser-136) expression, and may be an adverse predictor for NSCLC. Activation of the MET/phospho-BAD(Ser-136) signaling pathway might play a role in the development and progression of NSCLC.

  14. 78 FR 23935 - Federal Acquisition Regulation; Information Collection; Contractor Use of Interagency Fleet...

    Science.gov (United States)

    2013-04-23

    ...; Information Collection; Contractor Use of Interagency Fleet Management System Vehicles AGENCY: Department of... previously approved information collection requirement concerning contractor use of interagency fleet... Collection 9000- 0032, Contractor Use of Interagency Fleet Management System Vehicles, by any of the...

  15. 78 FR 64499 - Federal Acquisition Regulation; Submission for OMB Review; Contractor Use of Interagency Fleet...

    Science.gov (United States)

    2013-10-29

    ...; Submission for OMB Review; Contractor Use of Interagency Fleet Management System Vehicles AGENCY: Department... previously approved information collection requirement concerning contractor use of interagency fleet... comments identified by Information Collection 9000- 0032, Contractor Use of Interagency Fleet Management...

  16. Economics of symbiotic nuclear fleets at equilibrium

    International Nuclear Information System (INIS)

    Bidaud, Adrien; Guillemin, P.; Lecarpentier, David

    2008-01-01

    Many decades of industrial experience have proven that thermal reactors are able to provide a safe, reliable and competitive source of electricity. The higher construction costs of fast reactors compared to thermal reactors could be compensated by their better use of fissile material during the probable fast development of nuclear energy in the first half of the century. Thus, despite the over-cost of their cores, on the longer term, fast reactors are expected to take the lead in the nuclear reactor race. In the mean term, multi-strata symbiotic parks, using high conversion-rate thermal reactors, may delay fast reactor start up. We compare projected fuel cycle costs and cost of electricity of various symbiotic nuclear fleets, on the basis of a simple economic model and elementary costs estimated on publicly available data. These parameters and their evolution over reactor-life time scale can hardly be estimated. That is why we look at the sensitivities of our results to large modifications of the input parameters. The aim of our simple economic model is to understand which reactor characteristics should be optimized to enhance their economic performance when working as a single symbiotic fleet. (authors)

  17. Condition Monitoring of the SSE Generation Fleet

    Science.gov (United States)

    Twiddle, J.; Muthuraman, S.; Connolly, N.

    2012-05-01

    SSE (previously known as Scottish and Southern Energy) operates a diverse portfolio of generation plant, including coal, gas and renewable plant with a total generation capacity of 11,375MW (Sept 2011). In recent years a group of specialists dedicated to providing condition monitoring services has been established at the Equipment Performance Centre (EPC) based at Knottingley, West Yorkshire. We aim to illustrate the role of the EPC and the methods used for monitoring the generation fleet with the objective of maintaining asset integrity, reducing risk of plant failure and unplanned outages and describe the challenges which have been overcome in establishing the EPC. This paper describes methods including vibration and process data analysis, model-based techniques and on-site testing used for monitoring of generation plant, including gas turbines, steam turbines, generators and steam raising plant. These condition monitoring processes utilise available data, adding value to the business, by bringing services in-house and capturing knowledge of plant operation for the benefit of the whole fleet.

  18. Expression of Phospho-ELK1 and Its Prognostic Significance in Urothelial Carcinoma of the Upper Urinary Tract

    Directory of Open Access Journals (Sweden)

    Satoshi Inoue

    2018-03-01

    Full Text Available Using preclinical models, we have recently found that ELK1, a transcriptional factor that activates downstream targets, including c-fos proto-oncogene, induces bladder cancer outgrowth. Here, we immunohistochemically determined the expression status of phospho-ELK1, an activated form of ELK1, in upper urinary tract urothelial carcinoma (UUTUC. Overall, phospho-ELK1 was positive in 47 (47.5%; 37 weak (1+ and 10 moderate (2+ of 99 UUTUCs, which was significantly (P = 0.002 higher than in benign urothelium (21 (25.3% of 83; 17 1+ and 4 2+ and was also associated with androgen receptor expression (P = 0.001. Thirteen (35.1% of 37 non-muscle-invasive versus 34 (54.8% of 62 muscle-invasive UUTUCs (P = 0.065 were immunoreactive for phospho-ELK1. Lymphovascular invasion was significantly (P = 0.014 more often seen in phospho-ELK1(2+ tumors (80.0% than in phospho-ELK1(0/1+ tumors (36.0%. There were no statistically significant associations between phospho-ELK1 expression and tumor grade, presence of concurrent carcinoma in situ or hydronephrosis, or pN status. Kaplan-Meier and log-rank tests revealed that patients with phospho-ELK1(2+ tumor had marginally and significantly higher risks of disease progression (P = 0.055 and cancer-specific mortality (P = 0.008, respectively, compared to those with phospho-ELK1(0/1+ tumor. The current results thus support our previous observations in bladder cancer and further suggest that phospho-ELK1 overexpression serves as a predictor of poor prognosis in patients with UUTUC.

  19. Hydraulic Hybrid Fleet Vehicle Testing | Transportation Research | NREL

    Science.gov (United States)

    Hydraulic Hybrid Fleet Vehicle Evaluations Hydraulic Hybrid Fleet Vehicle Evaluations How Hydraulic Hybrid Vehicles Work Hydraulic hybrid systems can capture up to 70% of the kinetic energy that would -pressure reservoir to a high-pressure accumulator. When the vehicle accelerates, fluid in the high-pressure

  20. Uncertainty in Fleet Renewal: A Case from Maritime Transportation

    DEFF Research Database (Denmark)

    Pantuso, Giovanni; Fagerholt, Kjetil; Wallace, Stein W.

    2016-01-01

    This paper addresses the fleet renewal problem and particularly the treatment of uncertainty in the maritime case. A stochastic programming model for the maritime fleet renewal problem is presented. The main contribution is that of assessing whether or not better decisions can be achieved by using...

  1. Application of a greedy algorithm to military aircraft fleet retirements

    NARCIS (Netherlands)

    Newcamp, J.M.; Verhagen, W.J.C.; Udluft, H.; Curran, Ricky

    2017-01-01

    This article presents a retirement analysis model for aircraft fleets. By employing a greedy algorithm, the presented solution is capable of identifying individually weak assets in a fleet of aircraft with inhomogeneous historical utilization. The model forecasts future retirement scenarios

  2. A road safety performance indicator for vehicle fleet compatibility.

    NARCIS (Netherlands)

    Christoph, M. Vis, M.A. Rackliff, L. & Stipdonk, H.

    2013-01-01

    This paper discusses the development and the application of a safety performance indicator which measures the intrinsic safety of a country's vehicle fleet related to fleet composition. The indicator takes into account both the ‘relative severity’ of individual collisions between different vehicle

  3. Strategic vehicle fleet management - the make or buy problem

    Directory of Open Access Journals (Sweden)

    Adam Redmer

    2014-06-01

    Full Text Available Background: Fleets constitute the most important production means in transportation. Their appropriate management is crucial for all companies having transportation duties. The paper is the first one of a series of three papers that the author dedicates to the strategic vehicle fleet management topic. Methods: The paper discusses ways of fulfilling company's transportation needs (MAKE-or-BUY problem. It means the choice between using company's own and outside fleet (buying transportation services in a market. The essence of the MAKE-or-BUY problem lies in a time dependency, a seasonal nature of transportation needs. It leads to the MAKE-and-BUY solutions including utilization of both in-house and outside fleets. In the paper an original mathematical model (an optimization method allowing for the MAKE-and-BUY analysis is proposed. Results: An application of the proposed optimization method in a real-life decision situation (the case study within the Polish environment and the obtained solution are presented. The solution shows a low economic justification for using the MAKE option in practice. Especially when a fleet composed of brand new vehicles is considered. Conclusions: The paper will be continued in two further papers dedicated to strategic vehicle fleet management problems including fleet sizing / composition and fleet replacement.

  4. Plug-In Electric Vehicle Handbook for Fleet Managers (Brochure)

    Energy Technology Data Exchange (ETDEWEB)

    2012-04-01

    Plug-in electric vehicles (PEVs) are entering the automobile market and are viable alternatives to conventional vehicles. This guide for fleet managers describes the basics of PEV technology, PEV benefits for fleets, how to select the right PEV, charging a PEV, and PEV maintenance.

  5. The liquefied natural gas infrastructure and tanker fleet sizing problem

    DEFF Research Database (Denmark)

    Koza, David Franz; Røpke, Stefan; Molas, Anna Boleda

    2017-01-01

    We consider a strategic infrastructure and tanker fleet sizing problem in the liquefied natural gas business. The goal is to minimize long-term on-shore infrastructure and tanker investment cost combined with interrelated expected cost for operating the tanker fleet. A non-linear arc-based model...

  6. The Fleet Numerical Meteorology and Oceanography Center (FNMOC) - Naval

    Science.gov (United States)

    Meteorology Oceanography Ice You are here: Home › FNMOC FNMOC Logo FNMOC Navigation Meteorology Products Oceanography Products Tropical Applications Climatology and Archived Data Info The Fleet Numerical Meteorology and Oceanography Center (FNMOC) The Fleet Numerical Meteorology and Oceanography Center (FNMOC

  7. Avaliação da sensibilidade da goiabeira 'Pedro Sato' ao ozônio

    Directory of Open Access Journals (Sweden)

    Regina Maria de Moraes

    2011-09-01

    Full Text Available O objetivo deste trabalho foi avaliar a sensibilidade da goiabeira 'Pedro Sato' ao ozônio (O3, em comparação à cultivar Paluma, descrita como sensível. Oito plantas de cada cultivar foram submetidas separadamente a ar filtrado e a ar filtrado + O3, cinco horas por dia, durante quatro dias. Foram avaliadas fotossíntese (Asat, injúrias foliares visíveis e concentração de antocianinas e taninos. Plantas das duas cultivares também foram expostas, durante três meses, a condições ambientais em local contaminado por O3, tendo-se avaliado fotossíntese, injúrias foliares e crescimento. Plantas fumigadas com O3 apresentaram redução de Asat e da atividade fotoquímica, além de manifestação de injúrias foliares. 'Paluma' apresentou maior redução de Asat, injúrias foliares mais severas e redução de taninos. Quando exposta ao ambiente contaminado, 'Paluma' apresentou trocas gasosas mais altas, mas apresentou redução de Asat 30 dias antes que 'Pedro Sato'. O crescimento não foi afetado em 'Pedro Sato', cujas injúrias foliares tiveram menor incidência, severidade e precocidade do que em 'Paluma'. A menor taxa de crescimento de 'Paluma' indica maior sensibilidade ao O3. Apesar de responder com menor intensidade ao estresse induzido pelo O3, a cultivar Pedro Sato não pode ser considerada tolerante, pois apresenta danos fotoquímicos, injúrias foliares e alterações nos conteúdos de metabólitos secundários quando exposta a esse poluente.

  8. Phospho-Ser/Thr-binding domains: navigating the cell cycle and DNA damage response.

    Science.gov (United States)

    Reinhardt, H Christian; Yaffe, Michael B

    2013-09-01

    Coordinated progression through the cell cycle is a complex challenge for eukaryotic cells. Following genotoxic stress, diverse molecular signals must be integrated to establish checkpoints specific for each cell cycle stage, allowing time for various types of DNA repair. Phospho-Ser/Thr-binding domains have emerged as crucial regulators of cell cycle progression and DNA damage signalling. Such domains include 14-3-3 proteins, WW domains, Polo-box domains (in PLK1), WD40 repeats (including those in the E3 ligase SCF(βTrCP)), BRCT domains (including those in BRCA1) and FHA domains (such as in CHK2 and MDC1). Progress has been made in our understanding of the motif (or motifs) that these phospho-Ser/Thr-binding domains connect with on their targets and how these interactions influence the cell cycle and DNA damage response.

  9. Effects of phospho- and calciotropic hormones on electrolyte transport in the proximal tubule

    DEFF Research Database (Denmark)

    Lee, Justin J; Plain, Allein; Beggs, Megan R

    2017-01-01

    ), active vitamin D 3, and fibroblast growth factor 23 (FGF23). The organs central to this are the kidneys, intestine, and bone. In the kidney, the proximal tubule reabsorbs the majority of filtered calcium and phosphate, which amounts to more than 60% and 90%, respectively. The basic molecular mechanisms......Calcium and phosphate are critical for a myriad of physiological and cellular processes within the organism. Consequently, plasma levels of calcium and phosphate are tightly regulated. This occurs through the combined effects of the phospho- and calciotropic hormones, parathyroid hormone (PTH...... as their regulation of active vitamin D 3 synthesis in this nephron segment. The integrative effects of both phospho- and calciotropic hormones on proximal tubular solute transport and subsequently whole body calcium-phosphate balance thus have been further complicated. Here, we first review the molecular mechanisms...

  10. Guanylate kinase domains of the MAGUK family scaffold proteins as specific phospho-protein-binding modules.

    Science.gov (United States)

    Zhu, Jinwei; Shang, Yuan; Xia, Caihao; Wang, Wenning; Wen, Wenyu; Zhang, Mingjie

    2011-11-25

    Membrane-associated guanylate kinases (MAGUKs) are a large family of scaffold proteins that play essential roles in tissue developments, cell-cell communications, cell polarity control, and cellular signal transductions. Despite extensive studies over the past two decades, the functions of the signature guanylate kinase domain (GK) of MAGUKs are poorly understood. Here we show that the GK domain of DLG1/SAP97 binds to asymmetric cell division regulatory protein LGN in a phosphorylation-dependent manner. The structure of the DLG1 SH3-GK tandem in complex with a phospho-LGN peptide reveals that the GMP-binding site of GK has evolved into a specific pSer/pThr-binding pocket. Residues both N- and C-terminal to the pSer are also critical for the specific binding of the phospho-LGN peptide to GK. We further demonstrate that the previously reported GK domain-mediated interactions of DLGs with other targets, such as GKAP/DLGAP1/SAPAP1 and SPAR, are also phosphorylation dependent. Finally, we provide evidence that other MAGUK GKs also function as phospho-peptide-binding modules. The discovery of the phosphorylation-dependent MAGUK GK/target interactions indicates that MAGUK scaffold-mediated signalling complex organizations are dynamically regulated.

  11. Phospho-Pon Binding-Mediated Fine-Tuning of Plk1 Activity.

    Science.gov (United States)

    Zhu, Kang; Shan, Zelin; Zhang, Lu; Wen, Wenyu

    2016-07-06

    In Drosophila neuroblasts (NBs), the asymmetrical localization and segregation of the cell-fate determinant Numb are regulated by its adaptor Partner of Numb (Pon) and the cell-cycle kinase Polo. Polo phosphorylates the Pon localization domain, thus leading to its basal distribution together with Numb, albeit through an unclear mechanism. Here, we find that Cdk1 phosphorylates Pon at Thr63, thus creating a docking site for the Polo-box domain (PBD) of Polo-like kinase 1 (Plk1). The crystal structure of the Plk1 PBD/phospho-Pon complex reveals that two phospho-Pon bound PBDs associate to form a dimer of dimers. We provide evidence that phospho-Pon binding-induced PBD dimerization relieves the autoinhibition of Plk1. Moreover, we demonstrate that the priming Cdk1 phosphorylation of Pon is important for sequential Plk1 phosphorylation. Our results not only provide structural insight into how phosphoprotein binding activates Plk1 but also suggest that binding to different phosphoproteins might mediate the fine-tuning of Plk1 activity. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. Rapid phospho-turnover by receptor tyrosine kinases impacts downstream signaling and drug binding.

    Science.gov (United States)

    Kleiman, Laura B; Maiwald, Thomas; Conzelmann, Holger; Lauffenburger, Douglas A; Sorger, Peter K

    2011-09-02

    Epidermal growth factor receptors (ErbB1-4) are oncogenic receptor tyrosine kinases (RTKs) that regulate diverse cellular processes. In this study, we combine measurement and mathematical modeling to quantify phospho-turnover at ErbB receptors in human cells and to determine the consequences for signaling and drug binding. We find that phosphotyrosine residues on ErbB1 have half-lives of a few seconds and therefore turn over 100-1000 times in the course of a typical immediate-early response to ligand. Rapid phospho-turnover is also observed for EGF-activated ErbB2 and ErbB3, unrelated RTKs, and multiple intracellular adaptor proteins and signaling kinases. Thus, the complexes formed on the cytoplasmic tail of active receptors and the downstream signaling kinases they control are highly dynamic and antagonized by potent phosphatases. We develop a kinetic scheme for binding of anti-ErbB1 drugs to receptors and show that rapid phospho-turnover significantly impacts their mechanisms of action. Copyright © 2011 Elsevier Inc. All rights reserved.

  13. Detection and Cellular Localization of Phospho-STAT2 in the Central Nervous System by Immunohistochemical Staining

    DEFF Research Database (Denmark)

    Khorooshi, Reza; Owens, Trevor

    2013-01-01

    Phosphorylation of signal transducers and activators of transcription (STATs) indicates their involvement in active signaling. Here we describe immunohistochemical staining procedures for detection and identification of the cellular localization of phospho-STAT2 in the central nervous system (CNS...

  14. Saildrone fleet could help replace aging buoys

    Science.gov (United States)

    Voosen, Paul

    2018-03-01

    In April, two semiautonomous drones, developed by Saildrone, a marine tech startup based in Alameda, California, in close collaboration with the National Oceanic and Atmospheric Administration in Washington, D.C., are set to return from an 8-month tour of the Pacific Ocean. This the first scientific test for the drones, which are powered only by the wind and sun, in the Pacific Ocean. The voyage is an important step in showing that such drones, carrying 15 different sensors, could help replace an aging and expensive array of buoys that are the main way scientists sniff out signs of climate-disrupting El Niño events. If successful, scientists envision fleets of similar drones spreading across the ocean, inviting thoughts of what it could be like to do oceanography without a ship.

  15. How Packaging Fleet Renewal Fits French CEA Programs

    International Nuclear Information System (INIS)

    Dumesnil, J.; Malvache, P.; Hugon, F.C.; Sollacaro, M.

    2006-01-01

    CEA's (French Atomic Energy Agency) packaging fleet is dedicated to transportation of test irradiated fuels, of research reactors fuels, of navy propulsion fuels, and of waste coming from and to nuclear plants or facilities. This fleet encompasses more than 30 types of casks ranging from 5 to 30 tons, with either recent designs or other dating back to the seventies. A study has been launched in order to perform a global analysis of the life expectancy of the existing CEA and COGEMA Logistics cask fleets with respect to a 2015 target, in order to anticipate its renewal, while limiting the number of type of cask. Key elements like periodical evolutions of design and transport regulations, lessons learnt of existing casks (design, approval and extensions, operational feedback, maintenance and dismantling) are taken into account in order to ensure compliance and availability of the fleet. Moreover, from design to cask delivery, including regulatory tests, safety analysis report/ CoC, and manufacturing, 3 to 5 years is needed. Therefore cask development should be taken into account earlier of invest and research's programs. The paper will address the current life expectancy study of CEA and COGEMA Logistics packaging fleet, based on lessons learnt and regulation evolution and on general R and D plans by user facilities. It will show how a comprehensive optimized fleet is made available to CEA and other customers. Such a fleet combines optimized investment and uses, thus entailing synergies for well-mastered costs of transports. (authors)

  16. Municipal green fleet management in Ontario: best practices manual 2008

    Energy Technology Data Exchange (ETDEWEB)

    Felder, M.

    2008-07-01

    When households, institutional and commercial buildings, industry, and especially auto transportation use fossil fuel based energy, they generate carbon dioxide emissions. Considering the environmental concerns and the soaring fuel prices, this could be the opportunity for the municipal fleet operator to assume a leadership position regarding environmental issues and look for new cost efficiencies. To begin, a meticulous examination of the fuel expenditures, a good management approach of fleet operations and a clearly defined system or guidance for lowering fleet fuel costs would be helpful for municipal fleet managers. This document is involved in a pilot program aimed at helping Ontario municipalities understand and promote fleet efficiencies and obtain related environmental benefits. Existing and cost-effective automotive fleet management policies and practices that can mitigate pollution causing global warming are given in this guide. These policies and practices also allow money savings and contribute to a better workplace health and livability of the community. This document is based on the experience of fleet management experts and local governments in Ontario. 54 refs.

  17. CEP action area studies : City of Yellowknife fleet review

    International Nuclear Information System (INIS)

    2006-01-01

    The City of Yellowknife maintains a fleet of vehicles and equipment used for public service. Yellowknife's Community Energy Plan (CEP) has a goal to reduce Yellowknife's fleet fuel consumption and greenhouse gas (GHG) emissions by 20 per cent within 10 years. This report examined current fuel consumption for the City of Yellowknife's fleet based on available data and examined options to reduce fleet fuel consumption. It also quantified the reduction in fuel consumption, GHG emissions, as well as the financial impacts of various options. An overview of the City of Yellowknife's fleet of vehicles and equipment was first provided, as well as calculated annual values for fuel consumption, GHG emissions, and energy consumption for the City of Yellowknife's fleet. Two fleet management case studies were presented. Fuel-efficient vehicles on the market that could provide an opportunity to replace some of the City of Yellowknife's fleet of light vehicles, and bylaw vehicles with fuel-efficient vehicles were discussed. These vehicles include hybrid vehicles, smart cars, compressed natural gas vehicles, and compressed-air vehicles. Equipment maintenance for tires, preventive, synthetic oils, and driver training programs were discussed. Anti-idling campaigns and technologies were also examined. The report concluded with a discussion of renewable fuels such as biodiesel and ethanol blended gasoline. Fleet fuel consumption, GHG and financial impacts were provided for each chapter heading. It was concluded that if all of the measures identified in the report were implemented, an overall decrease in GHG emissions of approximately 15 per cent would be achieved. 15 refs., 8 tabs., 8 figs

  18. L’impossibile fuga. Soggetto migrante e dinamiche identitarie in The Peruvian notebooks di Braulio Muñoz

    Directory of Open Access Journals (Sweden)

    Rodja Bernardoni

    2011-06-01

    Full Text Available The article aims to examine the novel The peruvian notebooks, by the peruvian writer Braulio Muñoz. We will proceed by analyzing it through the ideas of migrant subject and migrant discourse developed by the critic Antonio Cornejo Polar. Starting from the instability and the fragmentation of these symbolic and discursive realities, we will try to point out how the text intends to investigate and to represent the alienating dynamics that, in the migration experience, mark the process of social and identitarian redefinition.

  19. Una aproximación a la biblioteca del ilustrado valenciano Juan Bautista Muñoz (1745-1799)

    OpenAIRE

    Bas Martín, Nicolás

    1998-01-01

    El presente estudio sobre la biblioteca del ilustrado valenciana Juan Bautista Muñoz (1745-1799) muestra las preocupaciones intelectuales que marcaron su trayectoria intelectual. Impulsor de la filosofía moderna en la Universidad de Valencia, fundador del Archivo General de Indias y autor de la Historia del Nuevo Mundo (1793), poseía una selecta y voluminosa biblioteca que contenía los libros más vanguardistas en las diversas disciplinas (filosofía, historia de América, religión, literatura, ...

  20. Preliminary assessment of fleets covered by the Energy Policy Act

    Energy Technology Data Exchange (ETDEWEB)

    Hu, P.S.; Davis, S.C.; Wang, M.Q. [and others

    1994-12-31

    To facilitate the goal of decreasing oil imports by 10 percent by the year 2000 and 30 percent by 2010, two sections of the Energy Policy Act encourage and mandate alternative fuel vehicles in the acquisition of fleet vehicles. The first step in estimating the contribution of these mandates toward meeting the aforementioned goal entails identifying affected fleets. This paper presents a preliminary assessment of potential vehicle fleet coverage. Only a limited number of companies in the methanol, ethanol, and hydrogen industries are likely to quality for this mandate. Whereas, many of the oil producers, petroleum refiners, and electricity companies are likely to be regulated.

  1. 41 CFR 101-39.104-1 - Consolidations into a fleet management system.

    Science.gov (United States)

    2010-07-01

    ... fleet management system. 101-39.104-1 Section 101-39.104-1 Public Contracts and Property Management..., TRANSPORTATION, AND MOTOR VEHICLES 39-INTERAGENCY FLEET MANAGEMENT SYSTEMS 39.1-Establishment, Modification, and Discontinuance of Interagency Fleet Management Systems § 101-39.104-1 Consolidations into a fleet management...

  2. 48 CFR 51.204 - Use of interagency fleet management system (IFMS) vehicles and related services.

    Science.gov (United States)

    2010-10-01

    ... Contractor Use of Interagency Fleet Management System (IFMS) 51.204 Use of interagency fleet management system (IFMS) vehicles and related services. Contractors authorized to use interagency fleet management... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Use of interagency fleet...

  3. 41 CFR 101-39.104 - Notice of establishment of a fleet management system.

    Science.gov (United States)

    2010-07-01

    ... of a fleet management system. 101-39.104 Section 101-39.104 Public Contracts and Property Management..., TRANSPORTATION, AND MOTOR VEHICLES 39-INTERAGENCY FLEET MANAGEMENT SYSTEMS 39.1-Establishment, Modification, and Discontinuance of Interagency Fleet Management Systems § 101-39.104 Notice of establishment of a fleet management...

  4. Wireless GPS fleet tracking system at the University of Albany.

    Science.gov (United States)

    2014-07-01

    This report provides an overview of the project undertaken at the University at Albany to make alternative transportation a more : viable option by implementing a GPS Tracking System on the University bus fleet and broadcasting the bus locations to c...

  5. Fleet Compliance Annual Report: Model Year 2015, Fiscal Year 2016

    Energy Technology Data Exchange (ETDEWEB)

    None

    2016-12-01

    The U.S. Department of Energy (DOE) regulates covered state government and alternative fuel provider fleets, pursuant to the Energy Policy Act of 1992 (EPAct), as amended. This report details compliance for model year 2015, fiscal year 2016.

  6. Risk Management of an Aging KC-130 Fleet

    National Research Council Canada - National Science Library

    Hoffman, Paul

    1999-01-01

    .... For the aging USMC KC-130 F/R fleet, a damage-tolerant approach ensured that safety would be maintained but it presented a problem in forecasting readiness, aircraft remaining in the inventory...

  7. Sweeney Todd : Fleet Streeti deemonlik habemeajaja / Kaisa Karu

    Index Scriptorium Estoniae

    Karu, Kaisa

    2008-01-01

    Lühiarvustus õudusmuusikalile "Sweeney Todd : Fleet Streeti deemonlik habemeajaja" (Stephen Sondheimi muusikali alusel), režissöör Tim Burton : peaosades Johnny Depp, Helena Bonham Carter : Ameerika Ühendriigid - Suurbritannia 2007

  8. DVD. Sweeney Todd : Fleet Streeti deemonlik habemeajaja / Egle Loo

    Index Scriptorium Estoniae

    Loo, Egle

    2008-01-01

    Lühiarvustus õudusmuusikalist "Sweeney Todd : The Demon Barber of Fleet Street" Stephen Sondheimi muusikali alusel : režissöör Tim Burton : peaosades Johnny Depp, Helena Bonham Carter : Ameerika Ühendriigid - Suurbritannia 2007

  9. Northeast Cooperative Research Study Fleet (SF) Program Biological Sampling Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Northeast Cooperative Research Study Fleet (SF) Program partners with a subset of commercial fishermen to collect high quality, high resolution, haul by haul...

  10. Fleet Protection Using a Small UAV Based IR Sensor

    National Research Council Canada - National Science Library

    Buss, James R; Ax, Jr, George R

    2005-01-01

    A study was performed to define candidate electro-optical and infrared (EO/IR) sensor configurations and assess their potential utility as small UAV-based sensors surveilling a perimeter around surface fleet assets...

  11. US Tune Purse Seine Fleet History & Activity Database

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NMFS SWR has collected historical vessel information on the U.S. tuna cannery baitboat and purse seine fleets for many years. The database's first record of a...

  12. Clinical application for the preservation of phospho-proteins through in-situ tissue stabilization

    Directory of Open Access Journals (Sweden)

    Ding Wei

    2010-11-01

    Full Text Available Abstract Background Protein biomarkers will play a pivotal role in the future of personalized medicine for both diagnosis and treatment decision-making. While the results of several pre-clinical and small-scale clinical studies have demonstrated the value of protein biomarkers, there have been significant challenges to translating these findings into routine clinical care. Challenges to the use of protein biomarkers include inter-sample variability introduced by differences in post-collection handling and ex vivo degradation of proteins and protein modifications. Results In this report, we re-create laboratory and clinical scenarios for sample collection and test the utility of a new tissue stabilization technique in preserving proteins and protein modifications. In the laboratory setting, tissue stabilization with the Denator Stabilizor T1 resulted in a significantly higher yield of phospho-protein when compared to standard snap freeze preservation. Furthermore, in a clinical scenario, tissue stabilization at collection resulted in a higher yield of total phospho-protein, total phospho-tyrosine, pErkT202/Y204 and pAktS473 when compared to standard methods. Tissue stabilization did not have a significant effect on other post-translational modifications such as acetylation and glycosylation, which are more stable ex-vivo. Tissue stabilization did decrease total RNA quantity and quality. Conclusion Stabilization at the time of collection offers the potential to better preserve tissue protein and protein modification levels, as well as reduce the variability related to tissue processing delays that are often associated with clinical samples.

  13. Business fleet refueling assessment. Final report, March 1992-November 1992

    International Nuclear Information System (INIS)

    Chaudier, A.

    1993-01-01

    The report investigates refueling characteristics and capabilities for the light-duty market sector, particularly business automobile fleets. It develops a profile of the characteristics of light-duty and automobile business fleets in 22 designated non-attainment areas, as well as Pittsburgh and Salt Lake City. Reliable data on these subjects will enable better analysis of the market potential for natural gas in the surveyed areas

  14. TQL in the Fleet: From Theory to Practice

    Science.gov (United States)

    1993-10-01

    distribution is unlimited. BEST-AVAILABLE Copy 94-04214 Total Quality Leadership , TQL, Total Quality Kanaginent, 191, i lo of pilo quality management ...703-602-8942). LINDA M. DOHERTY Director Department of the Navy Total Quality Leadership Office TOL In the Fleet: From Theory to Practice...the Fleet: From Theory to Practice ix OVERVIEW ) ver the past year, the Department of the Navy Total Quality Leadership Office (DON TQL Office) has

  15. Rapid Phospho-Turnover by Receptor Tyrosine Kinases Impacts Downstream Signaling and Drug Binding

    OpenAIRE

    Kleiman, Laura B.; Maiwald, Thomas; Conzelmann, Holger; Lauffenburger, Douglas A.; Sorger, Peter K.

    2011-01-01

    Epidermal growth factor receptors (ErbB1–4) are oncogenic receptor tyrosine kinases (RTKs) that regulate diverse cellular processes. In this study, we combine measurement and mathematical modeling to quantify phospho-turnover at ErbB receptors in human cells and to determine the consequences for signaling and drug binding. We find that phosphotyrosine residues on ErbB1 have half-lives of a few seconds and therefore turn over 100–1000 times in the course of a typical immediate-early response t...

  16. Detecting and categorizing fleeting emotions in faces.

    Science.gov (United States)

    Sweeny, Timothy D; Suzuki, Satoru; Grabowecky, Marcia; Paller, Ken A

    2013-02-01

    Expressions of emotion are often brief, providing only fleeting images from which to base important social judgments. We sought to characterize the sensitivity and mechanisms of emotion detection and expression categorization when exposure to faces is very brief, and to determine whether these processes dissociate. Observers viewed 2 backward-masked facial expressions in quick succession, 1 neutral and the other emotional (happy, fearful, or angry), in a 2-interval forced-choice task. On each trial, observers attempted to detect the emotional expression (emotion detection) and to classify the expression (expression categorization). Above-chance emotion detection was possible with extremely brief exposures of 10 ms and was most accurate for happy expressions. We compared categorization among expressions using a d' analysis, and found that categorization was usually above chance for angry versus happy and fearful versus happy, but consistently poor for fearful versus angry expressions. Fearful versus angry categorization was poor even when only negative emotions (fearful, angry, or disgusted) were used, suggesting that this categorization is poor independent of decision context. Inverting faces impaired angry versus happy categorization, but not emotion detection, suggesting that information from facial features is used differently for emotion detection and expression categorizations. Emotion detection often occurred without expression categorization, and expression categorization sometimes occurred without emotion detection. These results are consistent with the notion that emotion detection and expression categorization involve separate mechanisms. PsycINFO Database Record (c) 2013 APA, all rights reserved.

  17. Detecting and Categorizing Fleeting Emotions in Faces

    Science.gov (United States)

    Sweeny, Timothy D.; Suzuki, Satoru; Grabowecky, Marcia; Paller, Ken A.

    2013-01-01

    Expressions of emotion are often brief, providing only fleeting images from which to base important social judgments. We sought to characterize the sensitivity and mechanisms of emotion detection and expression categorization when exposure to faces is very brief, and to determine whether these processes dissociate. Observers viewed 2 backward-masked facial expressions in quick succession, 1 neutral and the other emotional (happy, fearful, or angry), in a 2-interval forced-choice task. On each trial, observers attempted to detect the emotional expression (emotion detection) and to classify the expression (expression categorization). Above-chance emotion detection was possible with extremely brief exposures of 10 ms and was most accurate for happy expressions. We compared categorization among expressions using a d′ analysis, and found that categorization was usually above chance for angry versus happy and fearful versus happy, but consistently poor for fearful versus angry expressions. Fearful versus angry categorization was poor even when only negative emotions (fearful, angry, or disgusted) were used, suggesting that this categorization is poor independent of decision context. Inverting faces impaired angry versus happy categorization, but not emotion detection, suggesting that information from facial features is used differently for emotion detection and expression categorizations. Emotion detection often occurred without expression categorization, and expression categorization sometimes occurred without emotion detection. These results are consistent with the notion that emotion detection and expression categorization involve separate mechanisms. PMID:22866885

  18. FLEET Velocimetry Measurements on a Transonic Airfoil

    Science.gov (United States)

    Burns, Ross A.; Danehy, Paul M.

    2017-01-01

    Femtosecond laser electronic excitation tagging (FLEET) velocimetry was used to study the flowfield around a symmetric, transonic airfoil in the NASA Langley 0.3-m TCT facility. A nominal Mach number of 0.85 was investigated with a total pressure of 125 kPa and total temperature of 280 K. Two-components of velocity were measured along vertical profiles at different locations above, below, and aft of the airfoil at angles of attack of 0 deg, 3.5 deg, and 7deg. Measurements were assessed for their accuracy, precision, dynamic range, spatial resolution, and overall measurement uncertainty in the context of the applied flowfield. Measurement precisions as low as 1 m/s were observed, while overall uncertainties ranged from 4 to 5 percent. Velocity profiles within the wake showed sufficient accuracy, precision, and sensitivity to resolve both the mean and fluctuating velocities and general flow physics such as shear layer growth. Evidence of flow separation is found at high angles of attack.

  19. Estimates of the radiation dose from phospho-gypsum plaster-board if used in domestic buildings

    International Nuclear Information System (INIS)

    O'Brien, R.S.; Peggie, J.R.; Leith, I.S.

    1991-02-01

    This report presents the results of a study carried out to estimate the annual effective dose equivalent contribution from phospho-gypsum plaster-board if it were used as an internal lining in buildings. The study considered four sources of radiation exposure that would arise in such use, such as inhalation of 222 Rn and its daughters, inhalation of phospho-gypsum dust and exposure to beta and gamma radiation. Measurements of the 22 6Ra content and 222 Rn exhalation rate were made for a number of samples of phospho-gypsum plaster-board, and the behaviour of 222 Rn and its daughters in a typical building was modelled. The results of the study suggest that, for building ventilation rates greater than approximately 0.5 air changes per hour, the contribution to the total annual effective dose equivalent from inhalation of radon ( 222 Rn) and its daughters ( 218 Po, 214 Pb, 214 Po) exhaled from the phospho-gypsum plaster-board should be well below the recommended limit of 1 milli-Sievert for members of the public. The total annual effective dose equivalent from all these sources should be less than 0.6 milli-Sieverts, provided reasonable work practices are observed during installation of the phospho-gypsum plaster-board and the ventilation rate is kept above approximately 0.5 air changes per hour. 31 refs., 12 tabs., 5 figs

  20. Académico Juan Jacobo Muñoz Delgado

    Directory of Open Access Journals (Sweden)

    Zoilo Cuellar Montoya

    1997-06-01

    Full Text Available

    En la hermosa tierra de Pubén, madre y nodriza de uno de los más encumbrados pueblos de Colombia; en Popayán, ciudad procera de la patria, vino a la vida el doctor Juan Jacobo Muñoz Delgado, el 9 de mayo de 1923.

    Aprendió sus primeras letras en la villa natal y luego viajó a la capital, donde se hizo Médico en la Facultad correspondiente de la Universidad Nacional de Bogotá. En 1947 recibió su grado, con honores, con un trabajo de tesis titulado, “Psicodiagnóstico de Rorschach. Aplicaciones Clínicas”.

    Entre 1947 y 1952 se especializó en cirugía oncológica en Nueva York y en Inglaterra y, a su regreso al país, en 1952, ingresó al Instituto Nacional de Cancerología, aún bajo la sabia Dirección del doctor César Augusto Pantoja, en el cual entró a formar parte del Departamento de Cirugía. En los años 1958 y 1959 ocupó la Jefatura del Departamento de Cirugía General del Hospital de La Samaritana y desde 1960, y por largo ‘tiempo, fue Jefe de Cirugía del Instituto Nacional de Cancerología. Ocupa también puestos directivos en diferentes instituciones asistenciales.

    Con una inclinación nata a la docencia, fue profesor de su especialidad en la Facultad de Medicina de la Universidad Nacional y en la de la Universidad Javeriana. Más tarde, entre 1965 y 1966, Ministro de Salud Pública y, posteriormente, entre 1971 y 1974, ocupó la cartera de Educación Nacional.

    Ingresó luego al campo diplomático y, en los años 1974 a 1977, fue Embajador de Colombia ante la UNESCO. Ya en Bogotá, se encargó de la Rectoría de la Universidad de los Andes entre 1978 y 1979, año en el cual, y en cumplimiento del Parágrafo 3 del Artículo 3 de la Ley 02 del 19 de enero de 1979, la Academia Nacional de Medicina 10 recibió como miembro Honorario, en su condición de ex Ministro de Salud.

    Entre 1979 y 19~1 ocupó la Dirección General del Instituto Colombiano de Bienestar Familiar, en la década de los

  1. Cyclones, bi-cycles, and psychoanalysis: the witch-of-us? complex and The Wizard of Oz.

    Science.gov (United States)

    Stern, Jeffrey

    2003-01-01

    Works of applied psychoanalysis normally use psychoanalytic theory to reveal the secret meanings of works of art. An attempt is made to reverse the directionality of such analyses and see whether a work of art, The Wizard of Oz, has something to teach psychoanalysis about adolescent female psychosexual development. The author argues that the popularity and importance of the film is an effect of its symbolic representation of a girl's entry into menarche, and the meaning of this milestone for herself and for her mother. He addresses ideas about feminine castration fears (or what more recently have been called fears of genital injury), issues about menopause, and fantasies--both surprising and violent--around the meanings of menarche for both mother and daughter.

  2. Interfacing the existing cask fleet with the MRS

    International Nuclear Information System (INIS)

    Doman, J.W.; Hahn, R.E.

    1992-01-01

    This paper reports that the Department of Energy (DOE) is considering the possibility of using the existing fleet of casks to achieve spent fuel receipt at the Monitored Retrievable Storage (MRS) facility. The existing cask fleet includes the NLI-1/2, the NAC-LWT, the TN-8 (and TN-8L), the TN-9, and the IF-300 casks. Other casks may be available, but their status is not certain. Use of the existing cask fleet at the MRS places additional design requirements on the system, and specifically affects the cask-to-MRS interface. The decision to use the existing cask fleet also places additional demands on training needs and operator certification, and the configuration management system. Some existing cask designs may not be able to mate with a bottom opening hot cell MRS. Use of the existing cask fleet also greatly increases the number of shipments that must be received, to the point that a facility larger than originally envisioned may be required

  3. Unraveling of the polymorphic C lambda 2-C lambda 3 amplification and the Ke+Oz- polymorphism in the human Ig lambda locus

    NARCIS (Netherlands)

    M. van der Burg (Mirjam); B.H. Barendregt (Barbara); E.J. van Gastel-Mol (Ellen); T. Tümkaya (Talip); A.W. Langerak (Anton); J.J.M. van Dongen (Jacques)

    2002-01-01

    textabstractTwo polymorphisms of the human Ig(lambda) (IGL) locus have been described. The first polymorphism concerns a single, 2- or 3-fold amplification of 5.4 kb of DNA in the C(lambda)2-C(lambda)3 region. The second polymorphism is the Mcg(-)Ke(+)Oz(-) isotype, which has

  4. Phospho-specific flow cytometry identifies aberrant signaling in indolent B-cell lymphoma

    Directory of Open Access Journals (Sweden)

    Blix Egil S

    2012-10-01

    Full Text Available Abstract Background Knowledge about signaling pathways in malignant cells may provide prognostic and diagnostic information in addition to identify potential molecular targets for therapy. B-cell receptor (BCR and co-receptor CD40 signaling is essential for normal B cells, and there is increasing evidence that signaling via BCR and CD40 plays an important role in the pathogenesis of B-cell lymphoma. The aim of this study was to investigate basal and induced signaling in lymphoma B cells and infiltrating T cells in single-cell suspensions of biopsies from small cell lymphocytic lymphoma/chronic lymphocytic leukemia (SLL/CLL and marginal zone lymphoma (MZL patients. Methods Samples from untreated SLL/CLL and MZL patients were examined for basal and activation induced signaling by phospho-specific flow cytometry. A panel of 9 stimulation conditions targeting B and T cells, including crosslinking of the B cell receptor (BCR, CD40 ligand and interleukins in combination with 12 matching phospho-protein readouts was used to study signaling. Results Malignant B cells from SLL/CLL patients had higher basal levels of phosphorylated (p-SFKs, p-PLCγ, p-ERK, p-p38, p-p65 (NF-κB, p-STAT5 and p-STAT6, compared to healthy donor B cells. In contrast, anti-BCR induced signaling was highly impaired in SLL/CLL and MZL B cells as determined by low p-SFK, p-SYK and p-PLCγ levels. Impaired anti-BCR-induced p-PLCγ was associated with reduced surface expression of IgM and CD79b. Similarly, CD40L-induced p-ERK and p-p38 were also significantly reduced in lymphoma B cells, whereas p-p65 (NF-κB was equal to that of normal B cells. In contrast, IL-2, IL-7 and IL-15 induced p-STAT5 in tumor-infiltrating T cells were not different from normal T cells. Conclusions BCR signaling and CD40L-induced p-p38 was suppressed in malignant B cells from SLL/CLL and MZL patients. Single-cell phospho-specific flow cytometry for detection of basal as well as activation

  5. Role of subsidies in EU fleet capacity management

    DEFF Research Database (Denmark)

    Lindebo, Erik

    2005-01-01

    Fisheries in the European Union (EU) continue to be overexploited by an overcapitalised fishing fleet, despite the best intentions of two decades of capacity adjustment programmes. This paper considers the progress of fishing capacity under the Multi-annual Guidance Programme and examines the imp...... of vessel decommissioning. The Danish fishing fleet case serves as an empirical example in this regard. Comments on the future capacity management regime and the role of subsidies in EU fisheries are offered.......Fisheries in the European Union (EU) continue to be overexploited by an overcapitalised fishing fleet, despite the best intentions of two decades of capacity adjustment programmes. This paper considers the progress of fishing capacity under the Multi-annual Guidance Programme and examines...

  6. Fleet Sizing of Automated Material Handling Using Simulation Approach

    Science.gov (United States)

    Wibisono, Radinal; Ai, The Jin; Ratna Yuniartha, Deny

    2018-03-01

    Automated material handling tends to be chosen rather than using human power in material handling activity for production floor in manufacturing company. One critical issue in implementing automated material handling is designing phase to ensure that material handling activity more efficient in term of cost spending. Fleet sizing become one of the topic in designing phase. In this research, simulation approach is being used to solve fleet sizing problem in flow shop production to ensure optimum situation. Optimum situation in this research means minimum flow time and maximum capacity in production floor. Simulation approach is being used because flow shop can be modelled into queuing network and inter-arrival time is not following exponential distribution. Therefore, contribution of this research is solving fleet sizing problem with multi objectives in flow shop production using simulation approach with ARENA Software

  7. Slow binding inhibition of phospho-N-acetylmuramyl-pentapeptide-translocase (Escherichia coli) by mureidomycin A.

    Science.gov (United States)

    Brandish, P E; Burnham, M K; Lonsdale, J T; Southgate, R; Inukai, M; Bugg, T D

    1996-03-29

    Enzymes of the membrane cycle of reactions in bacterial peptidoglycan biosynthesis remain as unexploited potential targets for antibacterial agents. The first of these enzymes, phospho-N-acetylmuramyl-pentapeptide-translocase (EC 2.7.8.13), has been overexpresed in Escherichia coli and solubilized from particulate fractions. The work of W.A. Weppner and F.C. Neuhaus ((1977) J. Biol. Chem. 252, 2296-303) has been extended to establish a usable routine fluorescence-based continuous assay for solubilized preparations. This assay has been used in the characterization of the natural product, mureidomycin A as a potent slow binding inhibitor of the enzyme with Ki and Ki* of 36 nM and 2 nM, respectively.

  8. FHA domains as phospho-threonine binding modules in cell signaling.

    Science.gov (United States)

    Hammet, Andrew; Pike, Brietta L; McNees, Carolyn J; Conlan, Lindus A; Tenis, Nora; Heierhorst, Jörg

    2003-01-01

    Forkhead-associated (FHA) domains are present in >200 diverse proteins in all phyla from bacteria to mammals and seem to be particularly prevalent in proteins with cell cycle control functions. Recent work from several laboratories has considerably improved our understanding of the structure and function of these domains that were virtually unknown a few years ago, and the first disease associations of FHA domains have now emerged. FHA domains form 11-stranded beta-sandwiches that contain some 100-180 amino acid residues with a high degree of sequence diversity. FHA domains act as phosphorylation-dependent protein-protein interaction modules that preferentially bind to phospho-threonine residues in their targets. Interestingly, point mutations in the human CHK2 gene that lead to single-residue amino acid substitutions in the FHA domain of this cell cycle checkpoint kinase have been found to cause a subset of cases of the Li-Fraumeni multi-cancer syndrome.

  9. Serum profiling of healthy aging identifies phospho- and sphingolipid species as markers of human longevity.

    Science.gov (United States)

    Montoliu, Ivan; Scherer, Max; Beguelin, Fiona; DaSilva, Laeticia; Mari, Daniela; Salvioli, Stefano; Martin, Francois-Pierre J; Capri, Miriam; Bucci, Laura; Ostan, Rita; Garagnani, Paolo; Monti, Daniela; Biagi, Elena; Brigidi, Patrizia; Kussmann, Martin; Rezzi, Serge; Franceschi, Claudio; Collino, Sebastiano

    2014-01-01

    As centenarians well represent the model of healthy aging, there are many important implications in revealing the underlying molecular mechanisms behind such successful aging. By combining NMR metabonomics and shot-gun lipidomics in serum we analyzed metabolome and lipidome composition of a group of centenarians with respect to elderly individuals. Specifically, NMR metabonomics profiling of serum revealed that centenarians are characterized by a metabolic phenotype distinct from that of elderly subjects, in particular regarding amino acids and lipid species. Shot- gun lipidomics approach displays unique changes in lipids biosynthesis in centenarians, with 41 differently abundant lipid species with respect to elderly subjects. These findings reveal phospho/sphingolipids as putative markers and biological modulators of healthy aging, in humans. Considering the particular actions of these metabolites, these data are suggestive of a better counteractive antioxidant capacity and a well-developed membrane lipid remodelling process in the healthy aging phenotype.

  10. Phospho-Caveolin-1 Mediates Integrin-Regulated Membrane Domain Internalisation

    Science.gov (United States)

    del Pozo, Miguel A.; Alderson, Nazilla B.; Grande-García, Araceli; Balasubramanian, Nagaraj; Schwartz, Martin A.; Kiosses, William B.; Anderson, Richard G.W.

    2005-01-01

    Growth of normal cells is anchorage-dependent because signalling through multiple pathways including Erk, PI 3-kinase and Rac requires integrin-mediated cell adhesion 1. Components of these pathways localize to low density, cholesterol-rich domains in the plasma membrane named “lipid rafts” 2,3 or “cholesterol enriched membrane microdomains” (CEMM) 4. We previously reported that integrin-mediated adhesion regulates CEMM trafficking such that cell detachment from the extracellular matrix (ECM) triggers CEMM internalisation and clearance from the plasma membrane 5. We now report that this internalisation is mediated by dynamin-2 and caveolin-1. Internalisation requires phosphorylation of caveolin-1 on tyrosine 14. A shift in localisation of phospho-caveolin-1 from focal adhesions to caveolae induces CEMM internalisation upon cell detachment, which mediates inhibition of Erk, PI 3-kinase and Rac. These data define a novel molecular mechanism for growth and tumour suppression by caveolin-1. PMID:16113676

  11. Kinomic and phospho-proteomic analysis of breast cancer stem-like cells

    DEFF Research Database (Denmark)

    Leth-Larsen, Rikke; Christensen, Anne Geske Lindhard; Ehmsen, Sidse

    Kinomic and phospho-proteomic analysis of breast cancer stem-like cells Rikke Leth-Larsen1, Anne G Christensen1, Sidse Ehmsen1, Mark Møller1, Giuseppe Palmisano2, Martin R Larsen2, Henrik J Ditzel1,3 1Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark 2Institute...... of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark 3Dept. of Oncology, Odense University Hospital, Odense, Denmark Cancer stem cells are thought to be responsible for tumorigenic potential and to possess resistance mechanisms against chemotherapy- and radiation-induced cancer...... cell death, while the bulk of a tumor lacks these capacities. The resistance mechanisms may cause these cells to survive and become the source of later tumor recurrence, highlighting the need for therapeutic strategies that specifically target pathways central to these cancer stem cells. The CD44hi...

  12. Phospho-Bcl-xL(Ser62) influences spindle assembly and chromosome segregation during mitosis.

    Science.gov (United States)

    Wang, Jianfang; Beauchemin, Myriam; Bertrand, Richard

    2014-01-01

    Functional analysis of a series of phosphorylation mutants reveals that Bcl-xL(Ser62Ala) influences cell entry into anaphase and mitotic exit in taxol-exposed cells compared with cells expressing wild-type Bcl-xL or a series of other phosphorylation mutants, an effect that appears to be independent of its anti-apoptotic activity. During normal mitosis progression, Bcl-xL(Ser62) is strongly phosphorylated by PLK1 and MAPK14/SAPKp38α at the prometaphase, metaphase, and the anaphase boundaries, while it is de-phosphorylated at telophase and cytokinesis. Phospho-Bcl-xL(Ser62) localizes in centrosomes with γ-tubulin and in the mitotic cytosol with some spindle-assembly checkpoint signaling components, including PLK1, BubR1, and Mad2. In taxol- and nocodazole-exposed cells, phospho-Bcl-xL(Ser62) also binds to Cdc20- Mad2-, BubR1-, and Bub3-bound complexes, while Bcl-xL(Ser62Ala) does not. Silencing Bcl-xL expression and expressing the phosphorylation mutant Bcl-xL(Ser62Ala) lead to an increased number of cells harboring mitotic spindle defects including multipolar spindle, chromosome lagging and bridging, aneuploidy with micro-, bi-, or multi-nucleated cells, and cells that fail to resolve undergo mitosis within 6 h. Together, the data indicate that during mitosis, Bcl-xL(Ser62) phosphorylation impacts on spindle assembly and chromosome segregation, influencing chromosome stability. Observations of mitotic cells harboring aneuploidy with micro-, bi-, or multi-nucleated cells, and cells that fail to resolve undergo mitosis within 6 h were also made with cells expressing the phosphorylation mutant Bcl-xL(Ser49Ala) and dual mutant Bcl-xL(Ser49/62Ala).

  13. Evaluation of Gear Condition Indicator Performance on Rotorcraft Fleet

    Science.gov (United States)

    Antolick, Lance J.; Branning, Jeremy S.; Wade, Daniel R.; Dempsey, Paula J.

    2010-01-01

    The U.S. Army is currently expanding its fleet of Health Usage Monitoring Systems (HUMS) equipped aircraft at significant rates, to now include over 1,000 rotorcraft. Two different on-board HUMS, the Honeywell Modern Signal Processing Unit (MSPU) and the Goodrich Integrated Vehicle Health Management System (IVHMS), are collecting vibration health data on aircraft that include the Apache, Blackhawk, Chinook, and Kiowa Warrior. The objective of this paper is to recommend the most effective gear condition indicators for fleet use based on both a theoretical foundation and field data. Gear diagnostics with better performance will be recommended based on both a theoretical foundation and results of in-fleet use. In order to evaluate the gear condition indicator performance on rotorcraft fleets, results of more than five years of health monitoring for gear faults in the entire HUMS equipped Army helicopter fleet will be presented. More than ten examples of gear faults indicated by the gear CI have been compiled and each reviewed for accuracy. False alarms indications will also be discussed. Performance data from test rigs and seeded fault tests will also be presented. The results of the fleet analysis will be discussed, and a performance metric assigned to each of the competing algorithms. Gear fault diagnostic algorithms that are compliant with ADS-79A will be recommended for future use and development. The performance of gear algorithms used in the commercial units and the effectiveness of the gear CI as a fault identifier will be assessed using the criteria outlined in the standards in ADS-79A-HDBK, an Army handbook that outlines the conversion from Reliability Centered Maintenance to the On-Condition status of Condition Based Maintenance.

  14. A road safety performance indicator for vehicle fleet compatibility.

    Science.gov (United States)

    Christoph, Michiel; Vis, Martijn Alexander; Rackliff, Lucy; Stipdonk, Henk

    2013-11-01

    This paper discusses the development and the application of a safety performance indicator which measures the intrinsic safety of a country's vehicle fleet related to fleet composition. The indicator takes into account both the 'relative severity' of individual collisions between different vehicle types, and the share of those vehicle types within a country's fleet. The relative severity is a measure for the personal damage that can be expected from a collision between two vehicles of any type, relative to that of a collision between passenger cars. It is shown how this number can be calculated using vehicle mass only. A sensitivity analysis is performed to study the dependence of the indicator on parameter values and basic assumptions made. The indicator is easy to apply and satisfies the requirements for appropriate safety performance indicators. It was developed in such a way that it specifically scores the intrinsic safety of a fleet due to its composition, without being influenced by other factors, like helmet wearing. For the sake of simplicity, and since the required data is available throughout Europe, the indicator was applied to the relative share of three of the main vehicle types: passenger cars, heavy goods vehicles and motorcycles. Using the vehicle fleet data from 13EU Member States and Norway, the indicator was used to rank the countries' safety performance. The UK was found to perform best in terms of its fleet composition (value is 1.07), while Greece has the worst performance with the highest indicator value (1.41). Copyright © 2013 Elsevier Ltd. All rights reserved.

  15. Fuel options for public bus fleets in Sweden

    OpenAIRE

    Xylia, Maria; Silveira, Semida

    2015-01-01

    The Swedish public transport sector has defined two major targets, i.e., to run 90% of the total vehicle kilometers of the fleet on non-fossil fuels and double the volume of travel via public transport by 2020, increasing the share of public transport in relation to the total personal transport in the country . The f3 report Fuel options for public bus fleets in Sweden highlights the challenges and solutions encountered, particularly when it comes to the adoption of renewable fuels in the reg...

  16. Staining Against Phospho-H2AX (gamma-H2AX) as a Marker for DNA Damage and Genomic Instability in Cancer Tissues and Cells

    NARCIS (Netherlands)

    Nagelkerke, A.P.; Span, P.N.

    2016-01-01

    Phospho-H2AX or gamma-H2AX- is a marker of DNA double-stranded breaks and can therefore be used to monitor DNA repair after, for example, irradiation. In addition, positive staining for phospho-H2AX may indicate genomic instability and telomere dysfunction in tumour cells and tissues. Here, we

  17. Effects of alkali metal cations on phospho-enzyme levels and [3H] ouabain binding to (Na+ + K+)-ATPase.

    Science.gov (United States)

    Han, C S; Tobin, T; Akera, T; Brody, T M

    1976-05-13

    The effects of several alkali metal cations on the relationship between steady state phospho-enzyme levels and initial velocity and equilibrium levels of [3H]-ouabain binding to (Na+ + K+)-ATPase (ATP phosphohydrolase EC 3.6.1.3.) were examined. Only Na+ increased both phospho-enzyme and [3H] ouabain binding levels above those observed in the presence of Mg2+ alone. While Na+ stimulated phosphorylation with an apparent Km of about 1 mM, its stimulation of [3H] ouabain binding was biphasic, the lower Km for stimulation corresponding to the Km for formation of phospho-enzyme. Among the other alkali metal cations, potassium, rubidium and lithium were at least eight times more effect in reducing phospho-enzyme levels than in reducing [3H] ouabain binding. This discrepancy is not due to the stability of the enzyme-ouabain complex, nor to any action on the rates of formation or dissociation of the enzyme-ouabain complex. The data thus suggest that [3H] ouabain interacts with the K+, Rb+ or Li+ -enzyme complexes. For Li+, this hypothesis is further supported by the observation that Li+ can cirectly increase the equilibrium level of [3H] ouabain binding to this enzyme under certain conditions.

  18. The Potential Impact of Collaborative and Three-Dimensional Imaging Technology on SHIPMAIN Fleet Modernization Plan

    National Research Council Canada - National Science Library

    Seaman, Nathan L; Housel, Thomas; Mun, Jonathan

    2008-01-01

    Maintenance and modernization of the US Navy fleet is big business. The Navy has invested substantial fiscal and human resources to standardize the processes used to accomplish maintenance modernization and repair for its fleet of ships...

  19. A Qualitative Examination of the Administrative Process of Fleet Enlisted Personnel in Various Medical Categories

    National Research Council Canada - National Science Library

    Weatherford, Lenora

    2003-01-01

    The purpose of this research is to examine the medical management process of placing and monitoring active duty fleet enlisted personnel in a temporary medical duty status and its impact on fleet readiness...

  20. Risk Assessment and Analysis of the M109 Family of Vehicles Fleet Management Pilot Program

    National Research Council Canada - National Science Library

    Hitz, Stephen

    1997-01-01

    ...) Fleet Management Pilot Program. The objective of this program is to reengineer the fleet's logistical support system by outsourcing those functions which make sense and that can be performed more efficiently by private industry...

  1. The Fleet Support Community: Meeting Its Mission in the 21st Century

    National Research Council Canada - National Science Library

    Murdy, Deanna

    1999-01-01

    This thesis evaluates the effectiveness of the Fleet Support community's management practices in meeting the dynamic changes in the complex fleet support arena, while increasing its value to the Navy in the future...

  2. The Retrofit Puzzle Extended: Optimal Fleet Owner Behavior over Multiple Time Periods

    Science.gov (United States)

    2009-08-04

    In "The Retrofit Puzzle: Optimal Fleet Owner Behavior in the Context of Diesel Retrofit Incentive Programs" (1) an integer program was developed to model profit-maximizing diesel fleet owner behavior when selecting pollution reduction retrofits. Flee...

  3. Commercial vehicle fleet management and information systems. Technical memorandum 2 : summary of case study interviews

    Science.gov (United States)

    1997-10-01

    The FHWA has commissioned the Commercial Vehicle Fleet Management and Information Systems study to determine if there are fleet management needs that the public sector can address through the development of ITS for commercial vehicle operations. As p...

  4. The Fleet-Management as an Element of the Modern Development in the Ukrainian Leasing Market

    OpenAIRE

    Garafonova Olga I.; Svynarchuk Anastasiia V.

    2017-01-01

    The article is aimed at researching practical aspects of the fleet-management development in Ukraine; relationships that arise in this process; elements that interact in the market. The preconditions of the companies’ transition to the use of this concept in the management of fleet, as well as the opportunities that arise in the enterprises using the principles of fleet-management were considered. The main advantages and disadvantages of the implementation of fleet-management in companies wit...

  5. Recruitment to the Norwegian fishing fleet: storylines, paradoxes, and pragmatism in Norwegian fisheries and recruitment policy

    OpenAIRE

    Sønvisen, Signe Annie

    2013-01-01

    The majority of actors in the Norwegian fisheries consider recruitment of fishers to be the main future challenge for the Norwegian fishing fleet. As fleet recruitment is a highly politicized field, the problem of how to mitigate the recruitment problem is a subject of heavy debate. Some argue that recruitment problems are caused by low fleet profitability, while others argue that recruitment problems are caused by fleet restructuring polices. This article aims to explore th...

  6. 75 FR 20778 - Security Zone; Portland Rose Festival Fleet Week, Willamette River, Portland, OR

    Science.gov (United States)

    2010-04-21

    ...-AA87 Security Zone; Portland Rose Festival Fleet Week, Willamette River, Portland, OR AGENCY: Coast... during the Portland Rose Festival Fleet Week from June 2, 2010, through June 7, 2010. The security zone... is a need to provide a security zone for the 2010 Portland Rose Festival Fleet Week, and there is...

  7. 41 CFR 101-39.105-1 - Transfers from discontinued or curtailed fleet management systems.

    Science.gov (United States)

    2010-07-01

    ... discontinued or curtailed fleet management systems. 101-39.105-1 Section 101-39.105-1 Public Contracts and... AVIATION, TRANSPORTATION, AND MOTOR VEHICLES 39-INTERAGENCY FLEET MANAGEMENT SYSTEMS 39.1-Establishment, Modification, and Discontinuance of Interagency Fleet Management Systems § 101-39.105-1 Transfers from...

  8. 48 CFR 970.5223-5 - DOE motor vehicle fleet fuel efficiency.

    Science.gov (United States)

    2010-10-01

    ... and Contract Clauses for Management and Operating Contracts 970.5223-5 DOE motor vehicle fleet fuel..., insert the following clause in contracts providing for Contractor management of the motor vehicle fleet... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false DOE motor vehicle fleet...

  9. 48 CFR 52.251-2 - Interagency Fleet Management System Vehicles and Related Services.

    Science.gov (United States)

    2010-10-01

    ... CLAUSES Text of Provisions and Clauses 52.251-2 Interagency Fleet Management System Vehicles and Related Services. As prescribed in 51.205, insert the following clause: Interagency Fleet Management System... to obtain interagency fleet management system vehicles and related services for use in the...

  10. 76 FR 1521 - Security Zone: Fleet Industrial Supply Center Pier, San Diego, CA

    Science.gov (United States)

    2011-01-11

    ...-AA87 Security Zone: Fleet Industrial Supply Center Pier, San Diego, CA AGENCY: Coast Guard, DHS. ACTION... Diego, CA. The existing security zone is around the former Fleet Industrial Supply Center Pier. The security zone encompasses all navigable waters within 100 feet of the former Fleet Industrial Supply Center...

  11. 41 CFR 102-34.340 - Do we need a fleet management information system?

    Science.gov (United States)

    2010-07-01

    ... management information system? 102-34.340 Section 102-34.340 Public Contracts and Property Management Federal... VEHICLE MANAGEMENT Federal Fleet Report § 102-34.340 Do we need a fleet management information system? Yes, you must have a fleet management information system at the department or agency level that — (a...

  12. Evolution of the deep-sea fleet that supports Canada's international trade

    Science.gov (United States)

    2002-01-01

    This study identifies the flag-related trends of fleets used in Canada's international sea-borne trade relative to the world fleet during the 15-year period from 1985 to 1999. The goal is to determine if there is any indication that fleets that serve...

  13. 75 FR 8563 - Safety Zone; Fleet Week Maritime Festival, Pier 66, Elliott Bay, Seattle, WA

    Science.gov (United States)

    2010-02-25

    ...-AA00 Safety Zone; Fleet Week Maritime Festival, Pier 66, Elliott Bay, Seattle, WA AGENCY: Coast Guard... Fleet Week Maritime Festival. This safety zone is necessary as these events have historically resulted... the safety of life and property on navigable waters during the annual Fleet Week Maritime Festival...

  14. Alternative Fuels Data Center: New Hampshire Fleet Revs up With Natural Gas

    Science.gov (United States)

    New Hampshire Fleet Revs up With Natural Gas to someone by E-mail Share Alternative Fuels Data Center: New Hampshire Fleet Revs up With Natural Gas on Facebook Tweet about Alternative Fuels Data Center: New Hampshire Fleet Revs up With Natural Gas on Twitter Bookmark Alternative Fuels Data Center

  15. Guidance for Federal Agencies on Executive Order 13693 - Federal Fleet Management

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2017-01-02

    Document contains guidance on the federal fleet requirements of Executive Order 13693: Planning for Federal Sustainability in the Next Decade and helps federal agencies subject to the executive order develop an overall approach for reducing total fleet greenhouse gas (GHG) emissions and fleet-wide per-mile GHG emissions.

  16. Waste Management's LNG Truck Fleet: Final Results

    Energy Technology Data Exchange (ETDEWEB)

    Chandler, K. [Battelle (US); Norton, P. [National Renewable Energy Laboratory (US); Clark, N. [West Virginia University (US)

    2001-01-25

    Waste Management, Inc., began operating a fleet of heavy-duty LNG refuse trucks at its Washington, Pennsylvania, facility. The objective of the project was to provide transportation professionals with quantitative, unbiased information on the cost, maintenance, operational, and emissions characteristics of LNG as one alternative to conventional diesel for heavy-duty trucking applications.

  17. Electric Vehicle Fleet Integration in the Danish EDISON Project

    DEFF Research Database (Denmark)

    Andersen, Peter Bach; Træholt, Chresten; Marra, Francesco

    2010-01-01

    The Danish EDISON project has been launched to investigate how a large fleet of electric vehicles (EVs) can be integrated in a way that supports the electric grid while benefitting both the individual car owners and society as a whole through reductions in CO2 emissions. The consortium partners...

  18. Scenario tree airline fleet planning for demand uncertainty

    NARCIS (Netherlands)

    Repko, M.G.J.; Lopes dos Santos, Bruno F.

    2017-01-01

    This paper proposes an innovative multi-period modeling approach to solve the airline fleet planning problem under demand uncertainty. The problem is modeled using a scenario tree approach. The tree is composed of nodes, which represent points of decision in multiple time stages of the planning

  19. Routing and scheduling and fleet management for liner shipping

    DEFF Research Database (Denmark)

    Kjeldsen, Karina Hjortshøj

    2009-01-01

    The problem of routing, scheduling and fleet management in global liner shipping is presented. The developed model incorporates the ships' speed as a decision variable. Furthermore, the model must be able to handle problems of the size and complexity experienced by the global liner shipping...

  20. Fleet Management Decision Making With Individual Aircraft Tracking Data

    NARCIS (Netherlands)

    Newcamp, Jeffrey; Verhagen, W.J.C.; Curran, R.

    2017-01-01

    Individual aircraft tracking data can be used by aircraft fleet managers to detect patterns in historical usage as a means to aid aging aircraft decision-making. This work tackles two aspects of applying these tracking data: investigating retirement patterns and assessing how base assignment can

  1. Report: EPA’s Fleet Management Program Needs Improvement

    Science.gov (United States)

    Report #15-P-0001, October 6, 2014. If oversight of the EPA’s fleet is not improved, the $6 million-per-year program could be ineffective and inefficient in supporting the agency’s mission and reporting data to the federal system.

  2. Solving the Liner Shipping Fleet Repositioning Problem with Cargo Flows

    DEFF Research Database (Denmark)

    Tierney, Kevin; Askelsdottir, Björg; Jensen, Rune Møller

    2015-01-01

    We solve a central problem in the liner shipping industry called the liner shipping fleet repositioning problem (LSFRP). The LSFRP poses a large financial burden on liner shipping firms. During repositioning, vessels are moved between routes in a liner shipping network. Liner carriers wish...

  3. Monitoring cod catches of the Dutch demersal fleet in 2016

    NARCIS (Netherlands)

    Hal, van R.; Machiels, M.A.M.

    2017-01-01

    This report presents the results of the cod monitoring program 2016. The research was commissioned by the Dutch Ministry of Economic Affairs within the EZ-program Beleidsondersteunend Onderzoek. Cod catches of the vessels in the fleet segments BT2 (beam trawl and pulse trawl) and TR (otter trawls

  4. Optimal charging schedule of an electric vehicle fleet

    DEFF Research Database (Denmark)

    Hu, Junjie; You, Shi; Østergaard, Jacob

    2011-01-01

    In this paper, we propose an approach to optimize the charging schedule of an Electric Vehicle (EV) fleet both taking into account spot price and individual EV driving requirement with the goal of minimizing charging costs. A flexible and suitable mathematic model is introduced to characterize...

  5. Applying Column Generation to the Discrete Fleet Planning Problem

    NARCIS (Netherlands)

    Bosman, M.G.C.; Bakker, Vincent; Molderink, Albert; Hurink, Johann L.; Smit, Gerardus Johannes Maria

    2010-01-01

    The paper discusses an Integer Linear Programming (ILP) formulation that describes the problem of planning the use of domestic distributed generators, under individual as well as fleet constraints. The planning problem comprises the assignment of time intervals during which the local generator must

  6. Using fleets of electric-drive vehicles for grid support

    International Nuclear Information System (INIS)

    Tomic, Jasna; Kempton, Willett

    2007-01-01

    Electric-drive vehicles can provide power to the electric grid when they are parked (vehicle-to-grid power). We evaluated the economic potential of two utility-owned fleets of battery-electric vehicles to provide power for a specific electricity market, regulation, in four US regional regulation services markets. The two battery-electric fleet cases are: (a) 100 Th.nk City vehicle and (b) 252 Toyota RAV4. Important variables are: (a) the market value of regulation services, (b) the power capacity (kW) of the electrical connections and wiring, and (c) the energy capacity (kWh) of the vehicle's battery. With a few exceptions when the annual market value of regulation was low, we find that vehicle-to-grid power for regulation services is profitable across all four markets analyzed. Assuming now more than current Level 2 charging infrastructure (6.6 kW) the annual net profit for the Th.nk City fleet is from US$ 7000 to 70,000 providing regulation down only. For the RAV4 fleet the annual net profit ranges from US$ 24,000 to 260,000 providing regulation down and up. Vehicle-to-grid power could provide a significant revenue stream that would improve the economics of grid-connected electric-drive vehicles and further encourage their adoption. It would also improve the stability of the electrical grid. (author)

  7. Optimal fleet conversion policy from a life cycle perspective

    International Nuclear Information System (INIS)

    Hyung Chul Kim; Ross, M.H.; Keoleian, G.A.

    2004-01-01

    Vehicles typically deteriorate with accumulating mileage and emit more tailpipe air pollutants per mile. Although incentive programs for scrapping old, high-emitting vehicles have been implemented to reduce urban air pollutants and greenhouse gases, these policies may create additional sales of new vehicles as well. From a life cycle perspective, the emissions from both the additional vehicle production and scrapping need to be addressed when evaluating the benefits of scrapping older vehicles. This study explores an optimal fleet conversion policy based on mid-sized internal combustion engine vehicles in the US, defined as one that minimizes total life cycle emissions from the entire fleet of new and used vehicles. To describe vehicles' lifetime emission profiles as functions of accumulated mileage, a series of life cycle inventories characterizing environmental performance for vehicle production, use, and retirement was developed for each model year between 1981 and 2020. A simulation program is developed to investigate ideal and practical fleet conversion policies separately for three regulated pollutants (CO, NMHC, and NO x ) and for CO 2 . According to the simulation results, accelerated scrapping policies are generally recommended to reduce regulated emissions, but they may increase greenhouse gases. Multi- objective analysis based on economic valuation methods was used to investigate trade-offs among emissions of different pollutants for optimal fleet conversion policies. (author)

  8. JSC Case Study: Fleet Experience with E-85 Fuel

    Science.gov (United States)

    Hummel, Kirck

    2009-01-01

    JSC has used E-85 as part of an overall strategy to comply with Presidential Executive Order 13423 and the Energy Policy Act. As a Federal fleet, we are required to reduce our petroleum consumption by 2 percent per year, and increase the use of alternative fuels in our vehicles. With the opening of our onsite dispenser in October 2004, JSC became the second federal fleet in Texas and the fifth NASA center to add E-85 fueling capability. JSC has a relatively small number of GSA Flex Fuel fleet vehicles at the present time (we don't include personal vehicles, or other contractor's non-GSA fleet), and there were no reasonably available retail E-85 fuel stations within a 15-minute drive or within five miles (one way). So we decided to install a small 1000 gallon onsite tank and dispenser. It was difficult to obtain a supplier due to our low monthly fuel consumption, and our fuel supplier contract has changed three times in less than five years. We experiences a couple of fuel contamination and quality control issues. JSC obtained good information on E-85 from the National Ethanol Vehicle Coalition (NEVC). We also spoke with Defense Energy Support Center, (DESC), Lawrence Berkeley Laboratory, and US Army Fort Leonard Wood. E-85 is a liquid fuel that is dispensed into our Flexible Fuel Vehicles identically to regular gasoline, so it was easy for our vehicle drivers to make the transition.

  9. Planning and forecasting demand for aircraft engines airline fleet

    Directory of Open Access Journals (Sweden)

    А.Г. Кучер

    2007-03-01

    Full Text Available  The questions of air-engines supply system processes analysis on the basis of order planning and air-engine demand forecasting of airline’s air fleet with the use of imitating simulation methods are considered.

  10. Alternative Fuels Data Center: Fleet Application for School Transportation

    Science.gov (United States)

    Propane Buses Jan. 26, 2016 Video thumbnail for Biodiesel Offers an Easy Alternative for Fleets Biodiesel thumbnail for Biodiesel Fuels Education in Alabama Biodiesel Fuels Education in Alabama May 1, 2012 Video School Transportation Videos on YouTube Video thumbnail for New Hampshire Cleans up with Biodiesel Buses

  11. The Liner Shipping Fleet Repositioning Problem with Cargo Flows

    DEFF Research Database (Denmark)

    Tierney, Kevin; Jensen, Rune Møller

    2012-01-01

    We solve an important problem for the liner shipping industry called the Liner Shipping Fleet Repositioning Problem (LSFRP). The LSFRP poses a large financial burden on liner shipping firms. During repositioning, vessels are moved between services in a liner shipping network. Shippers wish...

  12. PROBLEMS OF FORMATION OF RUSSIAN INNOVATIVE AGRICULTURAL TRACTORS FLEET

    Directory of Open Access Journals (Sweden)

    V. M. Kryazhkov

    2015-01-01

    Full Text Available Formation of tractors park and its perspective development are carried out on the basis of realization and improvement of the scientifically based types range constructed on a traction classification sign which conforms to requirements of agricultural production when performing two main conditions: full meeting the requirements for various standard sizes of tractors applicable to conditions of the corresponding zones of Russia and continuous reproduction and development of tractor fleet, both by quantity of efficient machinery and its structure. The developed types range practically runs the gamut of organizational and economic and climatic conditions, and also all range of forms of the organizations using machinery, beginning from individual consumers, small and large rent and contract collectives and finishing large-scale enterprises and their associations. Federal Service of State Statistics (Rosstat information about a steady tendency of fleet reduction was used for studying of regularities of development of tractor fleet. This information was added by analytical synthesis of data about the annual brand purchases in the Russian market of agricultural tractors. The developed database shows a share of the tractors in fleets of agricultural organizations, which are used over depreciation term, and also minimum necessary number of new machines introduction for formation of volume of tractors with limit age and the total quantity of tractors necessary for minimization of set of expenses. It was established that it is possible to estimate visually the developed quantitative and age structure of tractors fleet and to predict its development on prospect by worked out database representing set of annual tractors brand lines purchasing.

  13. Path Planning Algorithms for the Adaptive Sensor Fleet

    Science.gov (United States)

    Stoneking, Eric; Hosler, Jeff

    2005-01-01

    The Adaptive Sensor Fleet (ASF) is a general purpose fleet management and planning system being developed by NASA in coordination with NOAA. The current mission of ASF is to provide the capability for autonomous cooperative survey and sampling of dynamic oceanographic phenomena such as current systems and algae blooms. Each ASF vessel is a software model that represents a real world platform that carries a variety of sensors. The OASIS platform will provide the first physical vessel, outfitted with the systems and payloads necessary to execute the oceanographic observations described in this paper. The ASF architecture is being designed for extensibility to accommodate heterogenous fleet elements, and is not limited to using the OASIS platform to acquire data. This paper describes the path planning algorithms developed for the acquisition phase of a typical ASF task. Given a polygonal target region to be surveyed, the region is subdivided according to the number of vessels in the fleet. The subdivision algorithm seeks a solution in which all subregions have equal area and minimum mean radius. Once the subregions are defined, a dynamic programming method is used to find a minimum-time path for each vessel from its initial position to its assigned region. This path plan includes the effects of water currents as well as avoidance of known obstacles. A fleet-level planning algorithm then shuffles the individual vessel assignments to find the overall solution which puts all vessels in their assigned regions in the minimum time. This shuffle algorithm may be described as a process of elimination on the sorted list of permutations of a cost matrix. All these path planning algorithms are facilitated by discretizing the region of interest onto a hexagonal tiling.

  14. Genome-wide association study identifies novel loci associated with circulating phospho- and sphingolipid concentrations.

    Directory of Open Access Journals (Sweden)

    Ayşe Demirkan

    Full Text Available Phospho- and sphingolipids are crucial cellular and intracellular compounds. These lipids are required for active transport, a number of enzymatic processes, membrane formation, and cell signalling. Disruption of their metabolism leads to several diseases, with diverse neurological, psychiatric, and metabolic consequences. A large number of phospholipid and sphingolipid species can be detected and measured in human plasma. We conducted a meta-analysis of five European family-based genome-wide association studies (N = 4034 on plasma levels of 24 sphingomyelins (SPM, 9 ceramides (CER, 57 phosphatidylcholines (PC, 20 lysophosphatidylcholines (LPC, 27 phosphatidylethanolamines (PE, and 16 PE-based plasmalogens (PLPE, as well as their proportions in each major class. This effort yielded 25 genome-wide significant loci for phospholipids (smallest P-value = 9.88×10(-204 and 10 loci for sphingolipids (smallest P-value = 3.10×10(-57. After a correction for multiple comparisons (P-value<2.2×10(-9, we observed four novel loci significantly associated with phospholipids (PAQR9, AGPAT1, PKD2L1, PDXDC1 and two with sphingolipids (PLD2 and APOE explaining up to 3.1% of the variance. Further analysis of the top findings with respect to within class molar proportions uncovered three additional loci for phospholipids (PNLIPRP2, PCDH20, and ABDH3 suggesting their involvement in either fatty acid elongation/saturation processes or fatty acid specific turnover mechanisms. Among those, 14 loci (KCNH7, AGPAT1, PNLIPRP2, SYT9, FADS1-2-3, DLG2, APOA1, ELOVL2, CDK17, LIPC, PDXDC1, PLD2, LASS4, and APOE mapped into the glycerophospholipid and 12 loci (ILKAP, ITGA9, AGPAT1, FADS1-2-3, APOA1, PCDH20, LIPC, PDXDC1, SGPP1, APOE, LASS4, and PLD2 to the sphingolipid pathways. In large meta-analyses, associations between FADS1-2-3 and carotid intima media thickness, AGPAT1 and type 2 diabetes, and APOA1 and coronary artery disease were observed. In conclusion, our

  15. Historia, memoria y biografía en El jinete polaco de Anotnio Muñoz Molina

    Directory of Open Access Journals (Sweden)

    Felipe Oliver Fuentes Kraffczyk

    2012-12-01

    Full Text Available Los cruces y correspondencias del tiempo histórico y el tiempo biográfico es el sello característico de la ambiciosa novela de Antonio Muñoz Molina, El jinete polaco. Por intermediación del recuerdo el narrador fusiona su memoria individual en la memoria colectiva de Mágina, mítica población que sintetiza y condensa la crisis de una España atrapada en la encrucijada entre la tradición y la modernidad. De esta manera, el tiempo no avanza como un relato histórico convencional, como un conjunto de información lo suficientemente documentada y desplegada en una secuencia lineal, sino como un testimonio caótico que remueve nuevos lugares escondidos con celo dentro del yo. Dicho con otras palabras, a través de la subjetividad de un individuo que analiza en retrospectiva su experiencia personal, irrumpe en paralelo un mosaico fragmentario del pasado y presente de España. Este trabajo tiene por objeto revisar cómo por intermediación del lenguaje la novela logra entretejer una gran variedad de «emplazamientos»; entendiendo por emplazamiento la síntesis de «plaza» (lugar, y plazo (tiempo, y cómo estos binomios espacio-temporales re-construyen la personalidad de un yo.

  16. Alternative electrode placement in (automatic) sleep scoring (Fpz-Cz/Pz-Oz versus C4-A1).

    Science.gov (United States)

    van Sweden, B; Kemp, B; Kamphuisen, H A; Van der Velde, E A

    1990-06-01

    The purpose of this study is to investigate whether the international standard electrode placement (C4-A1) can be replaced by an alternative placement (Fpz-Cz/Pz-Oz) in an automatic sleep monitoring system without losing Rechtschaffen and Kales (R-K) balances. Single night-sleep polygraphic recordings of 10 patients, screened in a clinical sleep disorder setting, were recorded simultaneously with both placements, and visual sleep classification was performed separately by two independent observers. Interobserver and interplacement agreement were evaluated by way of average (dis)agreement matrices and kappa values computed for overall and individual stage scoring. Interobserver agreement for both the test and the standard electrode placements and interplacement agreement for both observers were assessed as fair to good or excellent. Scoring differences were evaluated by the rank sign test applied to clinical and theoretical difference scores. It appears that the interplacement differences are about equal to the interobserver differences, except for a slight tendency for sleep to be scored in a deeper stage with the proposed alternative placement. The data are presented and discussed in relation to current literature concepts.

  17. WozARd: A Wizard of Oz Method for Wearable Augmented Reality Interaction—A Pilot Study

    Directory of Open Access Journals (Sweden)

    Günter Alce

    2015-01-01

    Full Text Available Head-mounted displays and other wearable devices open up for innovative types of interaction for wearable augmented reality (AR. However, to design and evaluate these new types of AR user interfaces, it is essential to quickly simulate undeveloped components of the system and collect feedback from potential users early in the design process. One way of doing this is the wizard of Oz (WOZ method. The basic idea behind WOZ is to create the illusion of a working system by having a human operator, performing some or all of the system’s functions. WozARd is a WOZ method developed for wearable AR interaction. The presented pilot study was an initial investigation of the capability of the WozARd method to simulate an AR city tour. Qualitative and quantitative data were collected from 21 participants performing a simulated AR city tour. The data analysis focused on seven categories that can have an impact on how the WozARd method is perceived by participants: precision, relevance, responsiveness, technical stability, visual fidelity, general user-experience, and human-operator performance. Overall, the results indicate that the participants perceived the simulated AR city tour as a relatively realistic experience despite a certain degree of technical instability and human-operator mistakes.

  18. Tribological properties of BixTiyOz films grown via RF sputtering on 316L steel substrates

    Directory of Open Access Journals (Sweden)

    Johanna Parra

    2015-01-01

    Full Text Available En este trabajo se presentan los resultados obtenidos en el análisis químico superficial, la caracterización morfológica y evaluación de las propiedades tribológicas de recubrimientos de titanato de bismuto amorfo (BixTiyOz depositados sobre sustratos de acero inoxidable 316L utilizando la técnica de pulverización catódica rf. El análisis químico elemental se realizó por medio de espectroscopia de electrones Auger (EEA, la morfología de los recubrimientos se determinó mediante microscopia de fuerza atómica (MFA. Las medidas del coeficiente de fricción y la tasa de desgaste fueron obtenidas mediante pruebas de bola sobre disco. Los análisis de EEA permitieron establecer que los primeros 10 nm de los recubrimientos están formados probablemente por óxidos de Bi4Ti3O12 y Ti2O3, las medidas de AFM indican que los recubrimientos tienen una rugosidad promedio de 22.28nm y un tamaño de grano de 50nm. Finalmente, las pruebas tribológicas establecieron que el coeficiente de fricción y la tasa de desgaste del acero recubierto tiene valores similares al acero desnudo.

  19. The Spectrophotometric Sulfo-Phospho-Vanillin Assessment of Total Lipids in Human Meibomian Gland Secretions

    Science.gov (United States)

    McMahon, Anne; Lu, Hua

    2013-01-01

    Human meibomian gland secretions (meibum) are the major lipid component of the human preocular tear film. The predominant lipid classes found in meibum include waxes (WE), cholesteryl esters (CE), and varying amounts of cholesterol (Chl). The classical sulfo-phospho-vanillin assay (SPVA), adapted for a microplate reader, was used to quantitate lipids in meibum. To account for varying reactivities of different lipids in SPVA, a model meibomian lipid mixture (MMx) that approximated the WE/CE/Chl composition of meibum was developed and used to quantitate meibomian lipids. The overall SPV responses of MMx and meibum were found to be close, with similar intermediate and final reaction products for both. Saturated WE that had not been expected to be reactive were found to be SPV-positive. A reaction mechanism for these compounds in SPVA which involves the formation of alkenyl ethers is proposed and discussed. Tested proteins were non-reactive in SPVA. Thus, by comparing the results of gravimetric analyses of meibum samples with the results of a properly calibrated SPVA, it was estimated that the SPV-reactive lipid content of dry meibum in tested samples was about 78 % (w/w). The SPV method can also be adopted for analyzing other types of complex lipids secretions, such as sebum, as well as whole lipid extracts from other lipid-enriched organs and tissues, if proper standards are chosen. PMID:23345137

  20. redox reactions of uranium in the presence of potassium 2-phospho-17-tungstate

    International Nuclear Information System (INIS)

    Maslov, L.P.; Rykov, A.G.; Sirotinkina, L.V.

    1986-01-01

    The redox reactions of uranium in the presence of potassium 2-phospho-17-tungstate (17W2P) - K 10 P 2 W 17 O 61 - were studied by the methods of spectrophotometry and potentiometry. It was established that in the presence of 17W2P the UO /SUP 2/2/ + ion is reduced by iron(II) to uranium(IV) as a result of the binding of U(IV) into a strong complex with composition (U(p 2 W 17 O 61 ) 2 ) 16- . The peculiarities of the coordination of uranium(IV) by voluminous 17W2P ligands are the cause of the different nature of its interaction with various types of oxidizing agents. It was established that under the action of oxidizing agents (K 2 Cr 2 O 7 , KMnO 4 ), the reduced form of which is a complex with 17W2P, there is a conversion of the complex of uranium(IV) to the corresponding complex of uranium(V), evidently with conservation of a structure analogous to that for uranium(IV). It was also shown that uranium(IV) in complex with 17W2P is practically not oxidized in the cold by persulfate ions which react according to a radical mechanism, whereas weaker oxidizing agents, for example, H 2 O 2 , oxidize it to the UO /SUP 2/2/ + ion. Hypotheses are advanced on the possible mechanisms of the occurrence of the corresponding reactions

  1. Phospho switch triggers Brd4 chromatin binding and activator recruitment for gene-specific targeting.

    Science.gov (United States)

    Wu, Shwu-Yuan; Lee, A-Young; Lai, Hsien-Tsung; Zhang, Hong; Chiang, Cheng-Ming

    2013-03-07

    Bromodomain-containing protein 4 (Brd4) is an epigenetic reader and transcriptional regulator recently identified as a cancer therapeutic target for acute myeloid leukemia, multiple myeloma, and Burkitt's lymphoma. Although chromatin targeting is a crucial function of Brd4, there is little understanding of how bromodomains that bind acetylated histones are regulated, nor how the gene-specific activity of Brd4 is determined. Via interaction screen and domain mapping, we identified p53 as a functional partner of Brd4. Interestingly, Brd4 association with p53 is modulated by casein kinase II (CK2)-mediated phosphorylation of a conserved acidic region in Brd4 that selectively contacts either a juxtaposed bromodomain or an adjacent basic region to dictate the ability of Brd4 binding to chromatin and also the recruitment of p53 to regulated promoters. The unmasking of bromodomains and activator recruitment, concurrently triggered by the CK2 phospho switch, provide an intriguing mechanism for gene-specific targeting by a universal epigenetic reader. Copyright © 2013 Elsevier Inc. All rights reserved.

  2. Preparation and quantification of 3'-phosphoadenosine 5'-phospho[35S]sulfate with high specific activity

    International Nuclear Information System (INIS)

    Vargas, F.

    1988-01-01

    The synthesis and quantitation of the sulfate donor 3'-phosphoadenosine 5'-phospho[35S]sulfate (PAP35S), prepared from inorganic [35S]sulfate and ATP, were studied. An enzymatic transfer method based upon the quantitative transfer of [35S]sulfate from PAP35S to 2-naphthol and 4-methylumbelliferone by the action of phenolsulfotransferase activity from rat brain cytosol was also developed. The 2-naphthyl[35S]sulfate or 35S-methylumbelliferone sulfate formed was isolated by polystyrene bead chromatography. This method allows the detection of between 0.1 pmol and 1 nmol/ml of PAP35S. PAP35S of high specific activity (75 Ci/mmol) was prepared by incubating ATP and carrier-free Na2 35SO4 with a 100,000g supernatant fraction from rat spleen. The product was purified by ion-exchange chromatography. The specific activity and purity of PAP35S were estimated by examining the ratios of Km values for PAP35S of the tyrosyl protein sulfotransferase present in microsomes from rat cerebral cortex. The advantage and applications of these methods for the detection of femtomole amounts, and the synthesis of large scale quantities of PAP35S with high specific activity are discussed

  3. Medicago PhosphoProtein Database: a repository for Medicago truncatula phosphoprotein data

    Directory of Open Access Journals (Sweden)

    Christopher M. Rose

    2012-06-01

    Full Text Available The ability of legume crops to fix atmospheric nitrogen via a symbiotic association with soil rhizobia makes them an essential component of many agricultural systems. Initiation of this symbiosis requires protein phosphorylation-mediated signaling in response to rhizobial signals named Nod factors. Medicago truncatula (Medicago is the model system for studying legume biology, making the study of its phosphoproteome essential. Here, we describe the Medicago Phosphoprotein Database (http://phospho.medicago.wisc.edu, a repository built to house phosphoprotein, phosphopeptide, and phosphosite data specific to Medicago. Currently, the Medicago Phosphoprotein Database holds 3,457 unique phosphopeptides that contain 3,404 non-redundant sites of phosphorylation on 829 proteins. Through the web-based interface, users are allowed to browse identified proteins or search for proteins of interest. Furthermore, we allow users to conduct BLAST searches of the database using both peptide sequences and phosphorylation motifs as queries. The data contained within the database are available for download to be investigated at the user’s discretion. The Medicago Phosphoprotein Database will be updated continually with novel phosphoprotein and phosphopeptide identifications, with the intent of constructing an unparalleled compendium of large-scale Medicago phosphorylation data.

  4. An approach to the drone fleet survivability assessment based on a stochastic continues-time model

    Science.gov (United States)

    Kharchenko, Vyacheslav; Fesenko, Herman; Doukas, Nikos

    2017-09-01

    An approach and the algorithm to the drone fleet survivability assessment based on a stochastic continues-time model are proposed. The input data are the number of the drones, the drone fleet redundancy coefficient, the drone stability and restoration rate, the limit deviation from the norms of the drone fleet recovery, the drone fleet operational availability coefficient, the probability of the drone failure-free operation, time needed for performing the required tasks by the drone fleet. The ways for improving the recoverable drone fleet survivability taking into account amazing factors of system accident are suggested. Dependencies of the drone fleet survivability rate both on the drone stability and the number of the drones are analysed.

  5. Procesos de escritura en Beatus Ille (1986: primer estudio del “Archivo Personal de Antonio Muñoz Molina” (Arch. AMM/5/1.

    Directory of Open Access Journals (Sweden)

    Pablo Valdivia

    2015-11-01

    Full Text Available Beatus Ille (1986 fue la primera novela publicada de Antonio Muñoz Molina. En este artículo estudiamos, de manera pormenorizada, el contenido de la carpeta Arch. AMM/5/1 que se encuentra dentro del “Archivo Personal de Antonio Muñoz Molina” en la Biblioteca Nacional de España. Mediante el análisis de una importante cantidad de material inédito hemos reconstruido en este artículo los procesos de escritura sobre los que se levanta esta novela. En las siguientes páginas, explicamos el origen y el desarrollo de todo un conjunto de personajes, espacios, escenas y elementos fundamentales para la arquitectura narrativa de Beatus Ille. Gracias a este trabajo el lector especializado podrá comprender mejor algunas claves de lectura del conjunto de la producción de Muñoz Molina y, más concretamente, de la construcción ficcional de Beatus Ille.

  6. Utilização do Ozônio no Tratamento Pós-Colheita do Tomate (Lycopersicon esculentum Mill

    Directory of Open Access Journals (Sweden)

    Rogério Simão

    2011-06-01

    Full Text Available O armazenamento de hortaliças no período pós-colheita é fator preocupante na logística de produtos perecíveis no ramo do agronegócio. Este artigo tem como objetivo mostrar o emprego do gás Ozônio (O3 para o aumento de vida de prateleira de frutos de tomate a fim de mantê-los adequados por mais tempo para a comercialização. Exemplares da cultivar Débora foram tratados com 1 ppm (vol/vol de Ozônio durante 24 horas a 25°C e 65% de umidade relativa. Qualidades físicas como perda de massa, estádio de maturação, injúria e senescência foram avaliadas após 15 dias de armazenamento. Comparados com uma amostra de controle, os frutos tratados com Ozônio apresentaram menos perda de massa, menor porcentagem de injúrias causada por fungos e maior retardamento no apodrecimento, mas pouca diferença quanto ao nível de maturação.

  7. Phospho-BAD BH3 Mimicry Protects β Cells and Restores Functional β Cell Mass in Diabetes

    Directory of Open Access Journals (Sweden)

    Sanda Ljubicic

    2015-02-01

    Full Text Available Strategies that simultaneously enhance the survival and glucose responsiveness of insulin-producing β cells will greatly augment β cell replacement therapies in type 1 diabetes (T1D. We show that genetic and pharmacologic mimetics of the phosphorylated BCL-2 homology 3 (BH3 domain of BAD impart β-cell-autonomous protective effects in the face of stress stimuli relevant to β cell demise in T1D. Importantly, these benefits translate into improved engraftment of donor islets in transplanted diabetic mice, increased β cell viability in islet grafts, restoration of insulin release, and diabetes reversal. Survival of β cells in this setting is not merely due to the inability of phospho-BAD to suppress prosurvival BCL-2 proteins but requires its activation of the glucose-metabolizing enzyme glucokinase. Thus, BAD phospho-BH3 mimetics may prove useful in the restoration of functional β cell mass in diabetes.

  8. Specific membrane binding of factor VIII is mediated by O-phospho-L-serine, a moiety of phosphatidylserine.

    Science.gov (United States)

    Gilbert, G E; Drinkwater, D

    1993-09-21

    Phosphatidylserine, a negatively charged lipid, is exposed on the platelet membrane following cell stimulation, correlating with the expression of factor VIII receptors. We have explored the importance of the negative electrostatic potential of phosphatidylserine vs chemical moieties of phosphatidylserine for specific membrane binding of factor VIII. Fluorescein-labeled factor VIII bound to membranes containing 15% phosphatidic acid, a negatively charged phospholipid, with low affinity compared to phosphatidylserine-containing membranes. Binding was not specific as it was inhibited by other proteins in plasma. Factor VIII bound to membranes containing 10% phosphatidylserine in spite of a varying net charge provided by 0-15% stearylamine, a positively charged lipid. The soluble phosphatidylserine moiety, O-phospho-L-serine, inhibited factor VIII binding to phosphatidylserine-containing membranes with a Ki of 20 mM, but the stereoisomer, O-phospho-D-serine, was 5-fold less effective. Furthermore, binding of factor VIII to membranes containing synthetic phosphatidyl-D-serine was 5-fold less than binding to membranes containing phosphatidyl-L-serine. Membranes containing synthetic phosphatidyl-L-homoserine, differing from phosphatidylserine by a single methylene, supported high-affinity binding, but it was not specific as factor VIII was displaced by other plasma proteins. O-Phospho-L-serine also inhibited the binding of factor VIII to platelet-derived microparticles with a Ki of 20 mM, and the stereoisomer was 4-fold less effective. These results indicate that membrane binding of factor VIII is mediated by a stereoselective recognition O-phospho-L-serine of phosphatidylserine and that negative electrostatic potential is of lesser importance.

  9. 4-Acetamido-N-(λ5-triphenyl­phospho­ranyl­idene)benzene­sulfonamide

    Science.gov (United States)

    Prugovečki, Biserka; Marinković, Marina; Vinković, Mladen; Dumić, Miljenko

    2010-01-01

    There are two independent mol­ecules per asymmetric unit of the title compound, C26H23N2O3PS. Their superposition shows that they differ in the conformation of the CH3CO– group and the benzene rings from the triphenyl­phospho­rane group. In the crystal structure, independent mol­ecules are inter­conected by strong N—H⋯O hydrogen bonds, forming infinite chains along the a axis. PMID:21579151

  10. Aircraft Anomaly Detection Using Performance Models Trained on Fleet Data

    Science.gov (United States)

    Gorinevsky, Dimitry; Matthews, Bryan L.; Martin, Rodney

    2012-01-01

    This paper describes an application of data mining technology called Distributed Fleet Monitoring (DFM) to Flight Operational Quality Assurance (FOQA) data collected from a fleet of commercial aircraft. DFM transforms the data into aircraft performance models, flight-to-flight trends, and individual flight anomalies by fitting a multi-level regression model to the data. The model represents aircraft flight performance and takes into account fixed effects: flight-to-flight and vehicle-to-vehicle variability. The regression parameters include aerodynamic coefficients and other aircraft performance parameters that are usually identified by aircraft manufacturers in flight tests. Using DFM, the multi-terabyte FOQA data set with half-million flights was processed in a few hours. The anomalies found include wrong values of competed variables, (e.g., aircraft weight), sensor failures and baises, failures, biases, and trends in flight actuators. These anomalies were missed by the existing airline monitoring of FOQA data exceedances.

  11. CleanFleet. Final report: Volume 5, employee attitude assessment

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-12-01

    The experiences of couriers, operations managers, vehicle handlers (refuelers), and mechanics who drove and/or worked with alternative fuel vehicles, and the attitudes and perceptions of people with these experiences, are examined. Five alternative fuels studied in the CleanFleet project are considers& compressed natural gas, propane gas, California Phase 2 reformulated gasoline, M-85, and electricity. The three major areas of interest include comparative analysis of issues such as health, safety and vehicle performance, business issues encompassing several facets of station operations, and personal commentary and opinions about the CleanFleet project and the alterative fuels. Results of the employee attitude assessment are presented as both statistical and qualitative analysis.

  12. An SH2 domain model of STAT5 in complex with phospho-peptides define ``STAT5 Binding Signatures''

    Science.gov (United States)

    Gianti, Eleonora; Zauhar, Randy J.

    2015-05-01

    The signal transducer and activator of transcription 5 (STAT5) is a member of the STAT family of proteins, implicated in cell growth and differentiation. STAT activation is regulated by phosphorylation of protein monomers at conserved tyrosine residues, followed by binding to phospho-peptide pockets and subsequent dimerization. STAT5 is implicated in the development of severe pathological conditions, including many cancer forms. However, nowadays a few STAT5 inhibitors are known, and only one crystal structure of the inactive STAT5 dimer is publicly available. With a view to enabling structure-based drug design, we have: (1) analyzed phospho-peptide binding pockets on SH2 domains of STAT5, STAT1 and STAT3; (2) generated a model of STAT5 bound to phospho-peptides; (3) assessed our model by docking against a class of known STAT5 inhibitors (Müller et al. in ChemBioChem 9:723-727, 2008); (4) used molecular dynamics simulations to optimize the molecular determinants responsible for binding and (5) proposed unique "Binding Signatures" of STAT5. Our results put in place the foundations to address STAT5 as a target for rational drug design, from sequence, structural and functional perspectives.

  13. An SH2 domain model of STAT5 in complex with phospho-peptides define "STAT5 Binding Signatures".

    Science.gov (United States)

    Gianti, Eleonora; Zauhar, Randy J

    2015-05-01

    The signal transducer and activator of transcription 5 (STAT5) is a member of the STAT family of proteins, implicated in cell growth and differentiation. STAT activation is regulated by phosphorylation of protein monomers at conserved tyrosine residues, followed by binding to phospho-peptide pockets and subsequent dimerization. STAT5 is implicated in the development of severe pathological conditions, including many cancer forms. However, nowadays a few STAT5 inhibitors are known, and only one crystal structure of the inactive STAT5 dimer is publicly available. With a view to enabling structure-based drug design, we have: (1) analyzed phospho-peptide binding pockets on SH2 domains of STAT5, STAT1 and STAT3; (2) generated a model of STAT5 bound to phospho-peptides; (3) assessed our model by docking against a class of known STAT5 inhibitors (Müller et al. in ChemBioChem 9:723-727, 2008); (4) used molecular dynamics simulations to optimize the molecular determinants responsible for binding and (5) proposed unique "Binding Signatures" of STAT5. Our results put in place the foundations to address STAT5 as a target for rational drug design, from sequence, structural and functional perspectives.

  14. A strategy for interaction site prediction between phospho-binding modules and their partners identified from proteomic data.

    Science.gov (United States)

    Aucher, Willy; Becker, Emmanuelle; Ma, Emilie; Miron, Simona; Martel, Arnaud; Ochsenbein, Françoise; Marsolier-Kergoat, Marie-Claude; Guerois, Raphaël

    2010-12-01

    Small and large scale proteomic technologies are providing a wealth of potential interactions between proteins bearing phospho-recognition modules and their substrates. Resulting interaction maps reveal such a dense network of interactions that the functional dissection and understanding of these networks often require to break specific interactions while keeping the rest intact. Here, we developed a computational strategy, called STRIP, to predict the precise interaction site involved in an interaction with a phospho-recognition module. The method was validated by a two-hybrid screen carried out using the ForkHead Associated (FHA)1 domain of Rad53, a key protein of Saccharomyces cerevisiae DNA checkpoint, as a bait. In this screen we detected 11 partners, including Cdc7 and Cdc45, essential components of the DNA replication machinery. FHA domains are phospho-threonine binding modules and the threonines involved in both interactions could be predicted using the STRIP strategy. The threonines T484 and T189 in Cdc7 and Cdc45, respectively, were mutated and loss of binding could be monitored experimentally with the full-length proteins. The method was further tested for the analysis of 63 known Rad53 binding partners and provided several key insights regarding the threonines likely involved in these interactions. The STRIP method relies on a combination of conservation, phosphorylation likelihood, and binding specificity criteria and can be accessed via a web interface at http://biodev.extra.cea.fr/strip/.

  15. A Strategy for Interaction Site Prediction between Phospho-binding Modules and their Partners Identified from Proteomic Data*

    Science.gov (United States)

    Aucher, Willy; Becker, Emmanuelle; Ma, Emilie; Miron, Simona; Martel, Arnaud; Ochsenbein, Françoise; Marsolier-Kergoat, Marie-Claude; Guerois, Raphaël

    2010-01-01

    Small and large scale proteomic technologies are providing a wealth of potential interactions between proteins bearing phospho-recognition modules and their substrates. Resulting interaction maps reveal such a dense network of interactions that the functional dissection and understanding of these networks often require to break specific interactions while keeping the rest intact. Here, we developed a computational strategy, called STRIP, to predict the precise interaction site involved in an interaction with a phospho-recognition module. The method was validated by a two-hybrid screen carried out using the ForkHead Associated (FHA)1 domain of Rad53, a key protein of Saccharomyces cerevisiae DNA checkpoint, as a bait. In this screen we detected 11 partners, including Cdc7 and Cdc45, essential components of the DNA replication machinery. FHA domains are phospho-threonine binding modules and the threonines involved in both interactions could be predicted using the STRIP strategy. The threonines T484 and T189 in Cdc7 and Cdc45, respectively, were mutated and loss of binding could be monitored experimentally with the full-length proteins. The method was further tested for the analysis of 63 known Rad53 binding partners and provided several key insights regarding the threonines likely involved in these interactions. The STRIP method relies on a combination of conservation, phosphorylation likelihood, and binding specificity criteria and can be accessed via a web interface at http://biodev.extra.cea.fr/strip/. PMID:20733106

  16. Monitoring UV-induced signalling pathways in an ex vivo skin organ culture model using phospho-antibody array.

    Science.gov (United States)

    Lenain, Christelle; Gamboa, Bastien; Perrin, Agnes; Séraïdaris, Alexia; Bertino, Béatrice; Rival, Yves; Bernardi, Mathieu; Piwnica, David; Méhul, Bruno

    2018-05-01

    We investigated UV-induced signalling in an ex vivo skin organ culture model using phospho-antibody array. Phosphorylation modulations were analysed in time-course experiments following exposure to solar-simulated UV and validated by Western blot analyses. We found that UV induced P-p38 and its substrates, P-ERK1/2 and P-AKT, which were previously shown to be upregulated by UV in cultured keratinocytes and in vivo human skin. This indicates that phospho-antibody array applied to ex vivo skin organ culture is a relevant experimental system to investigate signalling events following perturbations. As the identified proteins are components of pathways implicated in skin tumorigenesis, UV-exposed skin organ culture model could be used to investigate the effect on these pathways of NMSC cancer drug candidates. In addition, we found that phospho-HCK is induced upon UV exposure, producing a new candidate for future studies investigating its role in the skin response to UV and UV-induced carcinogenesis. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  17. Effects of phospho- and calciotropic hormones on electrolyte transport in the proximal tubule [version 1; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Justin J. Lee

    2017-10-01

    Full Text Available Calcium and phosphate are critical for a myriad of physiological and cellular processes within the organism. Consequently, plasma levels of calcium and phosphate are tightly regulated. This occurs through the combined effects of the phospho- and calciotropic hormones, parathyroid hormone (PTH, active vitamin D3, and fibroblast growth factor 23 (FGF23. The organs central to this are the kidneys, intestine, and bone. In the kidney, the proximal tubule reabsorbs the majority of filtered calcium and phosphate, which amounts to more than 60% and 90%, respectively. The basic molecular mechanisms responsible for phosphate reclamation are well described, and emerging work is delineating the molecular identity of the paracellular shunt wherein calcium permeates the proximal tubular epithelium. Significant experimental work has delineated the molecular effects of PTH and FGF23 on these processes as well as their regulation of active vitamin D3 synthesis in this nephron segment. The integrative effects of both phospho- and calciotropic hormones on proximal tubular solute transport and subsequently whole body calcium-phosphate balance thus have been further complicated. Here, we first review the molecular mechanisms of calcium and phosphate reabsorption from the proximal tubule and how they are influenced by the phospho- and calciotropic hormones acting on this segment and then consider the implications on both renal calcium and phosphate handling as well as whole body mineral balance.

  18. Renewal of the French fleet of nuclear reactors

    International Nuclear Information System (INIS)

    Nifenecker, Herve

    2012-01-01

    While supposing the lifetime of all present PWR reactors would be extended to sixty years, the author compares two scenarios regarding the renewal of the French fleet of nuclear reactors: the first one over 40 years and the second one over 20 years. This renewal is based on the construction of EPR reactors at different rhythms. The author compares the associated production costs and assesses the exploitation costs. A renewal scenario over 40 years seems to give better results

  19. The ethanol heavy-duty truck fleet demonstration project

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-06-01

    This project was designed to test and demonstrate the use of a high- percentage ethanol-blended fuel in a fleet of heavy-duty, over-the- road trucks, paying particular attention to emissions, performance, and repair and maintenance costs. This project also represents the first public demonstration of the use of ethanol fuels as a viable alternative to conventional diesel fuel in heavy-duty engines.

  20. Optimal Routing for Heterogeneous Fixed Fleets of Multicompartment Vehicles

    OpenAIRE

    Wang, Qian; Ji, Qingkai; Chiu, Chun-Hung

    2014-01-01

    We present a metaheuristic called the reactive guided tabu search (RGTS) to solve the heterogeneous fleet multicompartment vehicle routing problem (MCVRP), where a single vehicle is required for cotransporting multiple customer orders. MCVRP is commonly found in delivery of fashion apparel, petroleum distribution, food distribution, and waste collection. In searching the optimum solution of MCVRP, we need to handle a large amount of local optima in the solution spaces. To overcome this proble...

  1. Controlled Hydrogen Fleet and Infrastructure Demonstration and Validation Project

    Energy Technology Data Exchange (ETDEWEB)

    Stottler, Gary

    2012-02-08

    General Motors, LLC and energy partner Shell Hydrogen, LLC, deployed a system of hydrogen fuel cell electric vehicles integrated with a hydrogen fueling station infrastructure to operate under real world conditions as part of the U.S. Department of Energy's Controlled Hydrogen Fleet and Infrastructure Validation and Demonstration Project. This technical report documents the performance and describes the learnings from progressive generations of vehicle fuel cell system technology and multiple approaches to hydrogen generation and delivery for vehicle fueling.

  2. Executive Order 13514: Federal Leadership in Environmental, Energy, and Economic Performance; Comprehensive Federal Fleet Management Handbook (Book)

    Energy Technology Data Exchange (ETDEWEB)

    Daley, R. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Ahdieh, N. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Bentley, J. [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2014-01-01

    A comprehensive Federal Fleet Management Handbook that builds upon the "Guidance for Federal Agencies on E.O. 13514 Section 12-Federal Fleet Management" and provides information to help fleet managers select optimal greenhouse gas and petroleum reduction strategies for each location, meeting or exceeding related fleet requirements, acquiring vehicles to support these strategies while minimizing fleet size and vehicle miles traveled, and refining strategies based on agency performance.

  3. The Fleet Application for Scheduling and Tracking (FAST) Management Website

    Science.gov (United States)

    Marrero-Perez, Radames J.

    2014-01-01

    The FAST application was designed to replace the paper and pen method of checking out and checking in GSA Vehicles at KSC. By innovating from a paper and pen based checkout system to a fully digital one, not only the resources wasted by printing the checkout forms have been reduced, but it also reduces significantly the time that users and fleet managers need to interact with the system as well as improving the record accuracy for each vehicle. The vehicle information is pulled from a centralized database server in the SPSDL. In an attempt to add a new feature to the FAST application, the author of this report (alongside the FAST developers) has been designing and developing the FAST Management Website. The GSA fleet managers had to rely on the FAST developers in order to add new vehicles, edit vehicles and previous transactions, or for generating vehicles reports. By providing an easy-to-use FAST Management Website portal, the GSA fleet managers are now able to easily move vehicles, edit records, and print reports.

  4. Field Operations Program Neighborhood Electric Vehicles - Fleet Survey

    Energy Technology Data Exchange (ETDEWEB)

    Francfort, James Edward; Carroll, M.

    2001-07-01

    This report summarizes a study of 15 automotive fleets that operate neighborhood electric vehicles(NEVs) in the United States. The information was obtained to help Field Operations Program personnel understand how NEVs are being used, how many miles they are being driven, and if they are being used to replace other types of fleet vehicles or as additions to fleets. (The Field Operations Program is a U.S. Department of Energy Program within the DOE Office of Energy Efficiency and Renewable Energy, Transportation Technologies). The NEVs contribution to petroleum avoidance and cleaner air can be estimated based on the miles driven and by assuming gasoline use and air emissions values for the vehicles being replaced. Gasoline and emissions data for a Honda Civic are used as the Civic has the best fuel use for a gasoline-powered vehicle and very clean emissions. Based on these conservation assumptions, the 348 NEVs are being driven a total of about 1.2 million miles per year. This equates to an average of 3,409 miles per NEV annually or 9 miles per day. It is estimated that 29,195 gallons of petroleum use is avoided annually by the 348 NEVs. This equates to 87 gallons of petroleum use avoided per NEV, per year. Using the 348 NEVs avoids the generation of at least 775 pounds of smog- forming emissions annually.

  5. Field Operations Program - Neighborhood Electric Vehicle Fleet Use

    Energy Technology Data Exchange (ETDEWEB)

    Francfort, J. E.; Carroll, M. R.

    2001-07-02

    This report summarizes a study of 15 automotive fleets that operate neighborhood electric vehicles (NEVs) in the United States. The information was obtained to help Field Operations Program personnel understand how NEVs are being used, how many miles they are being driven, and if they are being used to replace other types of fleet vehicles or as additions to fleets. (The Field Operations Program is a U.S. Department of Energy Program within the DOE Office of Energy Efficiency and Renewable Energy, Transportation Technologies). The NEVs contribution to petroleum avoidance and cleaner air can be estimated based on the miles driven and by assuming gasoline use and air emissions values for the vehicles being replaced. Gasoline and emissions data for a Honda Civic are used as the Civic has the best fuel use for a gasoline-powered vehicle and very clean emissions. Based on these conservation assumptions, the 348 NEVs are being driven a total of about 1.2 million miles per year. This equates to an average of 3,409 miles per NEV annually or 9 miles per day. It is estimated that 29,195 gallons of petroleum use is avoided annually by the 348 NEVs. This equates to 87 gallons of petroleum use avoided per NEV, per year. Using the 348 NEVs avoids the generation of at least 775 pounds of smog-forming emissions annually.

  6. Fleet renewal: An approach to achieve sustainable road transport

    Directory of Open Access Journals (Sweden)

    Manojlović Aleksandar V.

    2011-01-01

    Full Text Available With more stringent requirements for efficient utilization of energy resources within the transport industry a need for implementation of sustainable development principles has appeared. Such action will be one of competitive advantages in the future. This is especially confirmed within the road transport sector. A methodology implemented in public procurement procedures for fleet renewal regarding the calculation of road vehicles’ operational lifecycle costs has been analyzed in detail in this paper. Afore mentioned calculation comprises the costs for: vehicle ownership, energy, carbon dioxide and pollutants emissions. Implementation of this methodology allows making the choice of energy efficient vehicles and vehicles with notable positive environmental effects. The objective of the research is to assess the influence of specific parameters of vehicle operational lifecycle costs, especially energy costs and estimated vehicle energy consumption, on vehicle choice in the procurement procedure. The case of urban bus fleet in Serbia was analyzed. Their operational lifecycle costs were calculated and differently powered vehicles were assessed. Energy consumption input values were defined. It was proved that defined fleet renewal scenarios could influence unquestionable decrease in energy consumption.

  7. Phospho-kinase profile of triple negative breast cancer and androgen receptor signaling

    International Nuclear Information System (INIS)

    Cuenca-López, María D; Montero, Juan C; Morales, Jorge C; Prat, Aleix; Pandiella, Atanasio; Ocana, Alberto

    2014-01-01

    The androgen receptor (AR) plays a central role in the oncogenesis of different tumors, as is the case in prostate cancer. In triple negative breast cancer (TNBC) a gene expression classification has described different subgroups including a luminal androgen subtype. The AR can be controlled by several mechanisms like the activation of membrane tyrosine kinases and downstream signaling pathways. However little is known in TNBC about how the AR is modulated by these mechanisms and the potential therapeutic strategists to inhibit its expression. We used human samples to evaluate the expression of AR by western-blot and phospho-proteomic kinase arrays that recognize membrane tyrosine kinase receptors and downstream mediators. Western-blots in human cell lines were carried out to analyze the expression and activation of individual proteins. Drugs against these kinases in different conditions were used to measure the expression of the androgen receptor. PCR experiments were performed to assess changes in the AR gene after therapeutic modulation of these pathways. AR is present in a subset of TNBC and its expression correlates with activated membrane receptor kinases-EGFR and PDGFRβ in human samples and cell lines. Inhibition of the PI3K/mTOR pathway in TNBC cell lines decreased notably the expression of the AR. Concomitant administration of the anti-androgen bicalutamide with the EGFR, PDGFRβ and Erk1/2 inhibitors, decreased the amount of AR compared to each agent given alone, and had an additive anti-proliferative effect. Administration of dihydrotestosterone augmented the expression of AR that was not modified by the inhibition of the PI3K/mTOR or Erk1/2 pathways. AR expression was posttranscriptionally regulated by PI3K or Erk1/2 inhibition. Our results describe the expression of the AR in TNBC as a druggable target and further suggest the combination of bicalutamide with inhibitors of EGFR, PDGFRβ or Erk1/2 for future development

  8. A novel NSAID derivative, phospho-ibuprofen, prevents AOM-induced colon cancer in rats

    Science.gov (United States)

    OUYANG, NENGTAI; JI, PING; WILLIAMS, JENNIE L.

    2013-01-01

    The cancer chemopreventive properties and gastrointestinal toxicity of ibuprofen are well documented. Modification of existing NSAIDs has improved on the chemopreventive efficacy of this agent and reduced its toxicity. In this study, ibuprofen and a modified derivative (phospho-modified ibuprofen or p-ibuprofen) were used in a chemically induced model of colon cancer. Fisher 344 rats were injected with azoxymethane then treated with either ibuprofen (500 ppm) or p-ibuprofen (900 ppm) for 20 weeks to observe aberrant crypt foci (ACF) or 40 weeks to evaluate tumor incidence and multiplicity. β-catenin and p65 were measured in colonic tissues by immunofluorescence staining. Equal molar doses of ibuprofen (75 and 670 mg/kg) and p-ibuprofen (135 and 1,215 mg/kg) were administered to rats for 7 days to assess acute toxicity. The in vitro effect of p-ibuprofen on COX-2 and PGE2 synthesis, β-catenin expression and NF-κB activity were examined in RAW 264.7 macrophage and HCT 116 colon cancer cells. At week 20, p-ibuprofen and ibuprofen significantly reduced the multiplicity of ACF compared with control (pibuprofen and ibuprofen reduced the multiplicity of colon tumors compared with control (pibuprofen (670 mg/kg) and p-ibuprofen (1,215 mg/kg) resulted in stomach ulceration in 85.7% (6 out of 7) and 14.3% (1 out of 7) of rats, respectively, with pibuprofen and p-ibuprofen suppressed β-catenin nuclear translocation in colon cancer cells. In addition, p-ibuprofen but not ibuprofen inhibited NF-κB activation in colon cancer cells. Collectively, these results suggest that p-ibuprofen is a potential effective novel drug for long-term use in colon cancer prevention. PMID:23291777

  9. Desinfecção de efluente anaeróbio com o uso de ozônio/cloro

    Directory of Open Access Journals (Sweden)

    Gustavo Henrique Ribeiro da Silva

    2015-06-01

    Full Text Available O principal objetivo desta pesquisa foi estudar o processo de oxidação, a eficiência da inativação de coliformes e a formação de subprodutos da ozonização, associado com a desinfecção de efluente sanitário anaeróbio proveniente de um reator UASB. Tal evento foi realizado por meio da aplicação das dosagens de ozônio de 5,0 mg.O3.L-1+ 10 mg.Cl2.L-1 (ensaio 1; 8,0 mg.O3.L-1+ 20 mg.Cl2.L-1 (ensaio 2 e 10,0 mg.O3.L-1+ 30 mg.Cl2.L-1 (ensaio 3. Neste trabalho, utilizou-se efluente da Estação de Tratamento de Esgoto da Universidade de São Paulo, no Brasil. As reduções médias totais da demanda química de oxigênio para os efluentes ozonizados/clorados foram de 9, 32 e 37% para os ensaios 1, 2 e 3, respectivamente. A inativação dos coliformes totais variou de >5,7 até 6,4 log10 e >5,8 até >6,0 log10para Escherichia coli. A formação de aldeídos não sofreu mudanças significativas (p=0,05 para as dosagens e os tempos de contato aplicados após os ensaios de ozonização/cloração.

  10. Phospho-eNOS Ser-1176 is associated with the nucleoli and the Golgi complex in C6 rat glioma cells.

    Science.gov (United States)

    Klinz, Franz-Josef; Herberg, Natalie; Arnhold, Stefan; Addicks, Klaus; Bloch, Wilhelm

    2007-06-29

    Enzymatic activity of endothelial nitric oxide synthase (eNOS) is controlled by posttranslational modifications, protein-protein interactions, and subcellular localization. For example, N-terminal fatty acid modifications target eNOS to the Golgi complex where it becomes phosphorylated. We show here by immunofluorescence analysis that phospho-eNOS Ser-1176 is enriched in the perinuclear region of interphase C6 rat glioma cells. Confocal double immunofluorescence microscopy with the Golgi marker protein 58K revealed that phospho-eNOS Ser-1176 is associated with the Golgi complex. Surprisingly, we observed several spots in the nucleus of C6 cells that were positive for phospho-eNOS Ser-1176. Confocal double immunofluorescence analysis with the nucleolus marker protein fibrillarin revealed that within the nucleus phospho-eNOS Ser-1176 is exclusively associated with the nucleoli. It is known that in mitotic cells nucleoli are lost during prophase and rebuild during telophase. In agreement with this, we find no nucleoli-like distribution of phospho-eNOS Ser-1176 in metaphase and anaphase C6 glioma cells. Our finding that phospho-eNOS Ser-1176 is selectively associated with the nucleoli points to a so far unknown role for eNOS in interphase glioma cells.

  11. Optimization Model and Algorithm Design for Airline Fleet Planning in a Multiairline Competitive Environment

    Directory of Open Access Journals (Sweden)

    Yu Wang

    2015-01-01

    Full Text Available This paper presents a multiobjective mathematical programming model to optimize airline fleet size and structure with consideration of several critical factors severely affecting the fleet planning process. The main purpose of this paper is to reveal how multiairline competitive behaviors impact airline fleet size and structure by enhancing the existing route-based fleet planning model with consideration of the interaction between market share and flight frequency and also by applying the concept of equilibrium optimum to design heuristic algorithm for solving the model. Through case study and comparison, the heuristic algorithm is proved to be effective. By using the algorithm presented in this paper, the fleet operational profit is significantly increased compared with the use of the existing route-based model. Sensitivity analysis suggests that the fleet size and structure are more sensitive to the increase of fare price than to the increase of passenger demand.

  12. Size and transportation capabilities of the existing U.S. cask fleet

    International Nuclear Information System (INIS)

    Danese, F.L.; Johnson, P.E.; Joy, D.S.

    1990-01-01

    This paper investigates the current spent nuclear fuel cask fleet capability in the United States. It assesses the degree to which the current fleet would be available, as a contingency, until proposed Office of Civilian Radioactive Waste Management casks become operational. A limited fleet of ten spent fuel transportation casks is found to be readily available for use in Federal waste management efforts over the next decade

  13. Initial development of a practical safety audit tool to assess fleet safety management practices.

    Science.gov (United States)

    Mitchell, Rebecca; Friswell, Rena; Mooren, Lori

    2012-07-01

    Work-related vehicle crashes are a common cause of occupational injury. Yet, there are few studies that investigate management practices used for light vehicle fleets (i.e. vehicles less than 4.5 tonnes). One of the impediments to obtaining and sharing information on effective fleet safety management is the lack of an evidence-based, standardised measurement tool. This article describes the initial development of an audit tool to assess fleet safety management practices in light vehicle fleets. The audit tool was developed by triangulating information from a review of the literature on fleet safety management practices and from semi-structured interviews with 15 fleet managers and 21 fleet drivers. A preliminary useability assessment was conducted with 5 organisations. The audit tool assesses the management of fleet safety against five core categories: (1) management, systems and processes; (2) monitoring and assessment; (3) employee recruitment, training and education; (4) vehicle technology, selection and maintenance; and (5) vehicle journeys. Each of these core categories has between 1 and 3 sub-categories. Organisations are rated at one of 4 levels on each sub-category. The fleet safety management audit tool is designed to identify the extent to which fleet safety is managed in an organisation against best practice. It is intended that the audit tool be used to conduct audits within an organisation to provide an indicator of progress in managing fleet safety and to consistently benchmark performance against other organisations. Application of the tool by fleet safety researchers is now needed to inform its further development and refinement and to permit psychometric evaluation. Copyright © 2012 Elsevier Ltd. All rights reserved.

  14. A concise guide to effective management of the public works equipment fleet

    OpenAIRE

    De Armas, Vicente.

    1996-01-01

    CIVINS (Civilian Institutions) Thesis document Approved for public release ; distribution is unlimited The purpose of this report is to present in a concise form the principles and fundamentals of managing the public works equipment fleet, providing a basis from which to develop an effective fleet management program. The report begins with a review of today's trends in fleet management, including, organization, privatization, effects of environmental regulations and a discussion on info...

  15. Telematics Framework for Federal Agencies: Lessons from the Marine Corps Fleet

    Energy Technology Data Exchange (ETDEWEB)

    Hodge, Cabell [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Singer, Mark R. [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

    2017-10-24

    Executive Order 13693 requires federal agencies to acquire telematics for their light- and medium-duty vehicles as appropriate. This report is intended to help agencies that are deploying telematics systems and seeking to integrate them into their fleet management process. It provides an overview of telematics capabilities, lessons learned from the deployment of telematics in the Marine Corps fleet, and recommendations for federal fleet managers to maximize value from telematics.

  16. A multi-agent based intelligent configuration method for aircraft fleet maintenance personnel

    OpenAIRE

    Feng, Qiang; Li, Songjie; Sun, Bo

    2014-01-01

    A multi-agent based fleet maintenance personnel configuration method is proposed to solve the mission oriented aircraft fleet maintenance personnel configuration problem. The maintenance process of an aircraft fleet is analyzed first. In the process each aircraft contains multiple parts, and different parts are repaired by personnel with different majors and levels. The factors and their relationship involved in the process of maintenance are analyzed and discussed. Then the whole maintenance...

  17. Fleet Purchase Behavior: Decision Processes and Implications for New Vehicle Technologies and Fuels

    OpenAIRE

    Nesbitt, Kevin; Sperling, Daniel

    2001-01-01

    Vehicle fleets are a poorly understood part of the economy. They are important, though, in that they purchase a large share of light-duty vehicles and are often targeted by governments as agents of change. We investigate fleet purchase behavior, using focus groups, interviews, and mail and telephone surveys. We categorize fleets into four different decision-making structures (autocratic, bureaucratic, hierarchic, and democratic), determine what share of the market sector each represents, d...

  18. A Framework for Creating Value from Fleet Data at Ecosystem Level

    Science.gov (United States)

    Kinnunen, Sini-Kaisu; Hanski, Jyri; Marttonen-Arola, Salla; Kärri, Timo

    2017-09-01

    As companies have recently gotten more interested in utilizing the increasingly gathered data and realizing the potential of data analysis, the ability to upgrade data into value for business has been recognized as an advantage. Companies gain competitive advantage if they are able to benefit from the fleet data that is produced both in and outside the boundaries of the company. Benefits of fleet management are based on the possibility to have access to the massive amounts of asset data that can then be utilized e.g. to gain cost savings and to develop products and services. The ambition of the companies is to create value from fleet data but this requires that different actors in ecosystem are working together for a common goal - to get the most value out of fleet data for the ecosystem. In order that this could be possible, we need a framework to meet the requirements of the fleet life-cycle data utilization. This means that the different actors in the ecosystem need to understand their role in the fleet data refining process in order to promote the value creation from fleet data. The objective of this paper is to develop a framework for knowledge management in order to create value from fleet data in ecosystems. As a result, we present a conceptual framework which helps companies to develop their asset management practices related to the fleet of assets.

  19. Vehicular fleet operation on natural gas and propane: An overview. Final research report

    International Nuclear Information System (INIS)

    Taylor, D.B.; Mahmassani, H.; Euritt, M.A.

    1992-11-01

    The report attempts to contribute to the timely area of alternative vehicular fuels. It addresses the analysis of fleet operation on alternative fuels, specifically compressed natural gas (CNG) and propane, in terms of both fleet economics and societal impacts. Comprehensive information on engine technology, fueling infrastructure design, and societal impacts are presented. An evaluation framework useful for decisions between any vehicular fuels is developed. The comprehensive fleet cost-effectiveness analysis framework used in previous Project 983 reports is discussed in great detail. This framework/model is flexible enough to allow substantial sensitivity and scenario analysis. The model is used to perform sample analyses of both fleet economic and societal impacts

  20. A FRAMEWORK FOR CREATING VALUE FROM FLEET DATA AT ECOSYSTEM LEVEL

    Directory of Open Access Journals (Sweden)

    Sini-Kaisu KINNUNEN

    2017-07-01

    Full Text Available As companies have recently gotten more interested in utilizing the increasingly gathered data and realizing the potential of data analysis, the ability to upgrade data into value for business has been recognized as an advantage. Companies gain competitive advantage if they are able to benefit from the fleet data that is produced both in and outside the boundaries of the company. Benefits of fleet management are based on the possibility to have access to the massive amounts of asset data that can then be utilized e.g. to gain cost savings and to develop products and services. The ambi-tion of the companies is to create value from fleet data but this requires that different actors in ecosystem are working together for a common goal – to get the most value out of fleet data for the ecosystem. In order that this could be possi-ble, we need a framework to meet the requirements of the fleet life-cycle data utilization. This means that the different actors in the ecosystem need to understand their role in the fleet data refining process in order to promote the value creation from fleet data. The objective of this paper is to develop a framework for knowledge management in order to create value from fleet data in ecosystems. As a result, we present a conceptual framework which helps companies to develop their asset management practices related to the fleet of assets.

  1. Analysis of Fleet Readiness Center Southwest Concept Integration: New-Employee Orientation and Communication Processes

    National Research Council Canada - National Science Library

    Clemmons, Francini R; Falconieri, Holly M

    2007-01-01

    Fleet Readiness Center Southwest has embraced integration of personnel and processes from Aircraft Intermediate Maintenance Departments and Naval Aviation Depots supporting Naval Aviation Maintenance...

  2. Fleets of enduring drones to probe atmospheric phenomena with clouds

    Science.gov (United States)

    Lacroix, Simon; Roberts, Greg; Benard, Emmanuel; Bronz, Murat; Burnet, Frédéric; Bouhoubeiny, Elkhedim; Condomines, Jean-Philippe; Doll, Carsten; Hattenberger, Gautier; Lamraoui, Fayçal; Renzaglia, Alessandro; Reymann, Christophe

    2016-04-01

    A full spatio-temporal four-dimensional characterization of the microphysics and dynamics of cloud formation including the onset of precipitation has never been reached. Such a characterization would yield a better understanding of clouds, e.g. to assess the dominant mixing mechanism and the main source of cloudy updraft dilution. It is the sampling strategy that matters: fully characterizing the evolution over time of the various parameters (P, T, 3D wind, liquid water content, aerosols...) within a cloud volume requires dense spatial sampling for durations of the order of one hour. A fleet of autonomous lightweight UAVs that coordinate themselves in real-time as an intelligent network can fulfill this purpose. The SkyScanner project targets the development of a fleet of autonomous UAVs to adaptively sample cumuli, so as to provide relevant data to address long standing questions in atmospheric science. It mixes basic researches and experimental developments, and gathers scientists in UAV conception, in optimal flight control, in intelligent cooperative behaviors, and of course atmospheric scientists. Two directions of researches are explored: optimal UAV conception and control, and optimal control of a fleet of UAVs. The design of UAVs for atmospheric science involves the satisfaction of trade-offs between payload, endurance, ease of deployment... A rational conception scheme that integrates the constraints to optimize a series of criteria, in particular energy consumption, would yield the definition of efficient UAVs. This requires a fine modeling of each involved sub-system and phenomenon, from the motor/propeller efficiency to the aerodynamics at small scale, including the flight control algorithms. The definition of mission profiles is also essential, considering the aerodynamics of clouds, to allow energy harvesting schemes that exploit thermals or gusts. The conception also integrates specific sensors, in particular wind sensor, for which classic

  3. Phase equilibria and crystal chemistry of the CaO-½Gd2O3-CoOz system at 885 °C in air

    Science.gov (United States)

    Wong-Ng, W.; Laws, W.; Lapidus, S. H.; Ribaud, L.; Kaduk, J. A.

    2017-10-01

    The CaO-½Gd2O3-CoOz system prepared at 885 °C in air consists of two thermoelectric calcium cobaltate compounds, namely, the 2D thermoelectric oxide solid solution, (Ca3-xGdx)Co4O9-z (0 ≤ x ≤ 0.42) which has a misfit layered structure, and the 1D Ca3Co2O6 compound which consists of chains of alternating CoO6 trigonal prisms and CoO6 octahedra. Ca3Co2O6 was found to be a point compound. In the peripheral binary systems, Gd was not present in the Ca site of CaO, while a small solid solution region was identified for (Gd1-xCax)O(3-z)/2 (0 ≤ x ≤ 0.075). A solid solution region of distorted perovskite, (Gd1-xCax)CoO3-z (0 ≤ x ≤ 0.24, space group Pnma) was established. The structure of a member of the solid solution, (Gd0.92Ca0.08)CoO3-z, was determined using high resolution synchrotron radiation. A ternary oxide compound CaGdCoO4-z which has an orthorhombic structure (Bmab) was found to be stable at this temperature. Five solid solution tie-line regions and six three-phase regions were determined in the CaO-½Gd2O3-CoOz system. A comparison of the phase diagrams of the CaO-½R2O3-CoOz (R = La, Sm and Gd) systems is provided.

  4. Multiobjective optimization for nuclear fleet evolution scenarios using COSI

    Directory of Open Access Journals (Sweden)

    Freynet David

    2016-01-01

    Full Text Available The consequences of various fleet evolution options on material inventories and flux in fuel cycle and waste can be analysed by means of transition scenario studies. The COSI code is currently simulating chronologically scenarios whose parameters are fully defined by the user and is coupled with the CESAR depletion code. As the interactions among reactors and fuel cycle facilities can be complex, and the ways in which they may be configured are many, the development of optimization methodology could improve scenario studies. The optimization problem definition needs to list: (i criteria (e.g. saving natural resources and minimizing waste production; (ii variables (scenario parameters related to reprocessing, reactor operation, installed power distribution, etc.; (iii constraints making scenarios industrially feasible. The large number of scenario calculations needed to solve an optimization problem can be time-consuming and hardly achievable; therefore, it requires the shortening of the COSI computation time. Given that CESAR depletion calculations represent about 95% of this computation time, CESAR surrogate models have been developed and coupled with COSI. Different regression models are compared to estimate CESAR outputs: first- and second-order polynomial regressions, Gaussian process and artificial neural network. This paper is about a first optimization study of a transition scenario from the current French nuclear fleet to a Sodium Fast Reactors fleet as defined in the frame of the 2006 French Act for waste management. The present article deals with obtaining the optimal scenarios and validating the methodology implemented, i.e. the coupling between the simulation software COSI, depletion surrogate models and a genetic algorithm optimization method.

  5. Potential use of ozone in the food processingPotencialidades do uso do ozônio no processamento de alimentos

    Directory of Open Access Journals (Sweden)

    Mariana Curtinovi Geyer

    2011-07-01

    Full Text Available Ozone is a colorless and unstable gas with pungent smell and partially soluble in water, which is characterized by its high oxidant power. It is a strong agent disinfectant with high power germicidal properties acting in the inactivation of a wide variety of pathogenic organisms, including bacteria, virus and protozoa, presenting a germicidal efficiency that exceeds chlorine. Ozonization has become well-known in recent decades due to implementation of standards increasingly restrictive concerning the by-products of chlorination. Unlike chlorine, ozone do not form halogenated compounds with organic matter. However, it may form several other organic and inorganic. In the food industry, ozone can be use in sanitization processes of equipments and surfaces, as well as in the direct use on foodstuff in order to inactivate microorganisms and to extend shelf-life of food products.O ozônio é um gás incolor de odor pungente, instável e parcialmente solúvel em água, que se destaca por seu elevado poder oxidante. É um forte agente desinfetante com ação sobre uma grande variedade de organismos patogênicos, incluindo bactérias, vírus e protozoários, apresentado uma eficiência germicida que excede ao cloro. O uso do ozônio tornou-se notório nas últimas décadas em função da implementação de padrões cada vez mais restritos em relação aos subprodutos da cloração. Ao contrário do cloro, o ozônio não forma subprodutos halogenados com a matéria orgânica, mas pode induzir a formação de outros subprodutos orgânicos e inorgânicos. Na indústria de alimentos, o ozônio pode ser utilizado em processos de sanitização de superfícies e equipamentos, bem como no uso direto sobre as matérias-primas com o objetivo de inativar microrganismos e aumentar a vida-de-prateleira dos produtos alimentícios.

  6. Quality assurance in a cask fleet parts control system

    International Nuclear Information System (INIS)

    Fernandez, C.; Shappert, L.B.

    1992-01-01

    This paper discusses applicable portions of the eighteen Quality Assurance criteria of Subpart H, 10 CFR 71 which are incorporated into a relational data base system which has been designed to manage the spare parts control system for a fleet of spent nuclear fuel casks. The system includes not only parts in warehouse storage but parts in use in the field plus casks, ancillary equipment, test equipment, support devices, and even personnel. It provides a high degree of assurance that any device for which a condition for certification has expired will be flagged for recertification testing or removal from service well before the critical date

  7. Quality assurance in a cask fleet parts control system

    International Nuclear Information System (INIS)

    Fernandez, C.; McCreery, P.N.; Shappert, L.B.

    1991-01-01

    Applicable portions of the eighteen Quality Assurance criteria of Subpart H, 10 CFR 71 are incorporated into a relational data base system which has been designed to manage the spare parts control system for a fleet of spent nuclear fuel casks. The system includes not only parts in warehouse storage but parts in use in the field plus casks, ancillary equipment, test equipment, support devices, and even personnel. It provides a high degree of assurance that any device for which a condition for certification has expired will be flagged for recertification testing or removal from service well before the critical date

  8. Simultaneous Fleet Deployment and Network Design of Liner Shipping

    DEFF Research Database (Denmark)

    Gelareh, Shahin; Pisinger, David

    A mixed integer linear programming formulation is proposed for the simultaneous design of network and fleet deployment of a liner service providers for deep-sea shipping. The underlying network design problem is based on a 4-index (5-index by considering capacity type) formulation of the hub...... location problem which are known for their tightness. The demand is considered to be elastic in the sense that the service provider can accept any fraction of the origin-destination demand. We then propose a primal decomposition method to solve instances of the problem to optimality. Numerical results...... confirm superiority of our approach in comparison with a general-purpose mixed integer programming solver....

  9. Phospho-Rb mediating cell cycle reentry induces early apoptosis following oxygen-glucose deprivation in rat cortical neurons.

    Science.gov (United States)

    Yu, Ying; Ren, Qing-Guo; Zhang, Zhao-Hui; Zhou, Ke; Yu, Zhi-Yuan; Luo, Xiang; Wang, Wei

    2012-03-01

    The aim of this study was to investigate the relationship between cell cycle reentry and apoptosis in cultured cortical neurons following oxygen-glucose deprivation (OGD). We found that the percentage of neurons with BrdU uptake, TUNEL staining, and colocalized BrdU uptake and TUNEL staining was increased relative to control 6, 12 and 24 h after 1 h of OGD. The number of neurons with colocalized BrdU and TUNEL staining was decreased relative to the number of TUNEL-positive neurons at 24 h. The expression of phosphorylated retinoblastoma protein (phospho-Rb) was significantly increased 6, 12 and 24 h after OGD, parallel with the changes in BrdU uptake. Phospho-Rb and TUNEL staining were colocalized in neurons 6 and 12 h after OGD. This colocalization was strikingly decreased 24 h after OGD. Treatment with the cyclin-dependent kinase inhibitor roscovitine (100 μM) decreased the expression of phospho-Rb and reduced neuronal apoptosis in vitro. These results demonstrated that attempted cell cycle reentry with phosphorylation of Rb induce early apoptosis in neurons after OGD and there must be other mechanisms involved in the later stages of neuronal apoptosis besides cell cycle reentry. Phosphoralated Rb may be an important factor which closely associates aberrant cell cycle reentry with the early stages of neuronal apoptosis following ischemia/hypoxia in vitro, and pharmacological interventions for neuroprotection may be useful directed at this keypoint.

  10. Catalysis of the Oligomerization of O-Phospho-Serine, Aspartic Acid, or Glutamic Acid by Cationic Micelles

    Science.gov (United States)

    Bohler, Christof; Hill, Aubrey R., Jr.; Orgel, Leslie E.

    1996-01-01

    Treatment of relatively concentrated aqueous solutions of 0-phospho-serine (50 mM), aspartic acid (100 mM) or glutamic acid (100 mM) with carbonyldiimidazole leads to the formation of an activated intermediate that oligomerizes efficiently. When the concentration of amino acid is reduced tenfold, few long oligomers can be detected. Positively-charged cetyltrimethyl ammonium bromide micelles concentrate the negatively-charged activated intermediates of the amino acids at their surfaces and catalyze efficient oligomerization even from dilute solutions.

  11. Efeito do conteúdo e da natureza da lignina residual na eficiência e na seletividade do branqueamento com ozônio Effect of residual lignin content and nature on the efficiency and selectivity of ozone bleaching

    Directory of Open Access Journals (Sweden)

    Elenice Pereira Maia

    2003-04-01

    Full Text Available Neste estudo foram avaliados os efeitos do conteúdo e da natureza da lignina residual na eficiência e na seletividade do branqueamento com ozônio de polpa kraft convencional (kraft e pré-deslignificada com oxigênio (kraft-O. Constatou-se que a eficiência do branqueamento com ozônio se eleva com o aumento do conteúdo de lignina residual da polpa. O tratamento com ozônio é mais seletivo para polpas kraft-O, mas para um mesmo tipo de polpa a seletividade de branqueamento com ozônio se eleva com o aumento de lignina residual. A eficiência do branqueamento com ozônio aumenta com o teor de lignina fenólica na polpa, entretanto a seletividade é negativamente afetada pela presença destas estruturas.This study aimed to evaluate the effect of residual lignin content and nature on the efficiency and selectivity of ozone bleaching of conventional (kraft and oxygen delignified (kraft-O pulps. Ozone bleaching efficiency was found to be enhanced by increasing pulp residual lignin content. Ozone treatment is more selective for kraft-O pulps, but for a given type of pulp (kraft or kraft-O, ozone bleaching selectivity increases with increasing pulp lignin content. Ozone bleaching efficiency increases with increasing pulp lignin phenolic hydroxyl content whereas selectivity is negatively affected by these structures.

  12. Cooperative aquatic sensing using the telesupervised adaptive ocean sensor fleet

    Science.gov (United States)

    Dolan, John M.; Podnar, Gregg W.; Stancliff, Stephen; Low, Kian Hsiang; Elfes, Alberto; Higinbotham, John; Hosler, Jeffrey; Moisan, Tiffany; Moisan, John

    2009-09-01

    Earth science research must bridge the gap between the atmosphere and the ocean to foster understanding of Earth's climate and ecology. Typical ocean sensing is done with satellites or in situ buoys and research ships which are slow to reposition. Cloud cover inhibits study of localized transient phenomena such as Harmful Algal Blooms (HAB). A fleet of extended-deployment surface autonomous vehicles will enable in situ study of characteristics of HAB, coastal pollutants, and related phenomena. We have developed a multiplatform telesupervision architecture that supports adaptive reconfiguration based on environmental sensor inputs. Our system allows the autonomous repositioning of smart sensors for HAB study by networking a fleet of NOAA OASIS (Ocean Atmosphere Sensor Integration System) surface autonomous vehicles. In situ measurements intelligently modify the search for areas of high concentration. Inference Grid and complementary information-theoretic techniques support sensor fusion and analysis. Telesupervision supports sliding autonomy from high-level mission tasking, through vehicle and data monitoring, to teleoperation when direct human interaction is appropriate. This paper reports on experimental results from multi-platform tests conducted in the Chesapeake Bay and in Pittsburgh, Pennsylvania waters using OASIS platforms, autonomous kayaks, and multiple simulated platforms to conduct cooperative sensing of chlorophyll-a and water quality.

  13. The Russian Northern Fleet. Sources of radioactive contamination

    Energy Technology Data Exchange (ETDEWEB)

    Nilsen, T [Bellona Foundation, Oslo (Norway); Kudrik, I [Bellona Foundation Branch Office, Murmansk (Russian Federation); Nikitin, A [Scientific Production Association ` ` Typhoon` ` , Obninsk (Russian Federation)

    1996-08-01

    The report describes the problems that the Russian Northern Fleet is experiencing with its nuclear powered vessels and with the storage of spent fuel and other nuclear wastes that the operation of these vessels generates. One of the most serious problems is the lack of regional storage and treatment facilities for radioactive waste. This waste is now deposited haphazardly throughout the various navy yards and bases. The establishment of a regional storage facility for spent fuel, radioactive reactor components, and liquid and solid nuclear waste is a necessary precondition for carrying out the decommissioning of nuclear submarines in an environmentally viable manner. A recurrent theme in the report is the lack of civilian control over the different Northern Fleet nuclear facilities. This leads to a disregard of international recommendations with regard to the handling of nuclear waste. Considerable effort has been made to provide comprehensive references in the report, making it clear that the authors sources of information have been open. By presenting this information the authors hope to contribute to increased insight and consequently to help realize necessary national and international measures. 93 refs.

  14. IMPROVING LOGISTICS SERVICES THROUGH THE TECHNOLOGY USED IN FLEET MANAGEMENT

    Directory of Open Access Journals (Sweden)

    Mauro Vivaldini

    2012-12-01

    Full Text Available Outsourcing logistics has established itself in the area of the LSP (Logistics Service Provider, which offers a range of services to its customers. In this line, transportation is characterized as one of the most important services, and therefore efficient fleet management is essential for establishing a high level of customer service. With advances in technology and vehicle tracking systems, this approach of management has gained new possibilities for the improvement of logistics services. By studying the specific case of an LSP, this paper investigates the use of these technologies in the management of their business and services. The results indicate that the LSP seeks to increase its services and to streamline information in order to respond to customer needs in real time. It is also evident in this case under study that the combination of the technology available together with the fleet management system has become a distinguishing feature for this LSP, one which increases their skills and important information for both customers and business.

  15. The Russian Northern Fleet. Sources of radioactive contamination

    International Nuclear Information System (INIS)

    Nilsen, T.; Kudrik, I.; Nikitin, A.

    1996-08-01

    The report describes the problems that the Russian Northern Fleet is experiencing with its nuclear powered vessels and with the storage of spent fuel and other nuclear wastes that the operation of these vessels generates. One of the most serious problems is the lack of regional storage and treatment facilities for radioactive waste. This waste is now deposited haphazardly throughout the various navy yards and bases. The establishment of a regional storage facility for spent fuel, radioactive reactor components, and liquid and solid nuclear waste is a necessary precondition for carrying out the decommissioning of nuclear submarines in an environmentally viable manner. A recurrent theme in the report is the lack of civilian control over the different Northern Fleet nuclear facilities. This leads to a disregard of international recommendations with regard to the handling of nuclear waste. Considerable effort has been made to provide comprehensive references in the report, making it clear that the authors sources of information have been open. By presenting this information the authors hope to contribute to increased insight and consequently to help realize necessary national and international measures. 93 refs

  16. Process Approach for Modeling of Machine and Tractor Fleet Structure

    Science.gov (United States)

    Dokin, B. D.; Aletdinova, A. A.; Kravchenko, M. S.; Tsybina, Y. S.

    2018-05-01

    The existing software complexes on modelling of the machine and tractor fleet structure are mostly aimed at solving the task of optimization. However, the creators, choosing only one optimization criterion and incorporating it in their software, provide grounds on why it is the best without giving a decision maker the opportunity to choose it for their enterprise. To analyze “bottlenecks” of machine and tractor fleet modelling, the authors of this article created a process model, in which they included adjustment to the plan of using machinery based on searching through alternative technologies. As a result, the following recommendations for software complex development have been worked out: the introduction of a database of alternative technologies; the possibility for a user to change the timing of the operations even beyond the allowable limits and in that case the calculation of the incurred loss; the possibility to rule out the solution of an optimization task, and if there is a necessity in it - the possibility to choose an optimization criterion; introducing graphical display of an annual complex of works, which could be enough for the development and adjustment of a business strategy.

  17. Antonio Muñoz (1884-1960 and the history of Byzantine illumination: a new field of research in Italy under the aegis of Adolfo Venturi

    Directory of Open Access Journals (Sweden)

    Giovanni Gasbarri

    2015-12-01

    Full Text Available Antonio Muñoz (1884-1960 is usually remembered for his successful career in the administration and conservation of the monumental heritage in Rome during the Fascist era. His brief yet relevant activity as a Byzantinist is often underestimated in the secondary literature, despite the fact that, before the 1914-18 war, he was unanimously considered as the most promising Italian specialist in Byzantine art. From 1903 onwards, after completing his studies with Adolfo Venturi, Muñoz developed an interest in Byzantine illumination, which became his primary area of expertise. At this early stage, his most important work was without doubt the new edition of the Rossano Gospels (1907, which was internationally praised as a significant step forward in the history of Byzantine illumination. With the support of previously unpublished or little-known documents, this article aims to provide a new critical analysis of Muñoz’s research on Byzantine illumination, as well as a more balanced evaluation of its impact on the scholarly community at the turn of the twentieth century.

  18. Electric and Plug-In Hybrid Electric Fleet Vehicle Testing | Transportation

    Science.gov (United States)

    Research | NREL Electric and Plug-In Hybrid Electric Fleet Vehicle Evaluations Electric and Plug-In Hybrid Electric Fleet Vehicle Evaluations How Electric and Plug-In Hybrid Electric Vehicles plugging the vehicle into an electric power source. PHEVs are powered by an internal combustion engine that

  19. The Fleet-Management as an Element of the Modern Development in the Ukrainian Leasing Market

    Directory of Open Access Journals (Sweden)

    Garafonova Olga I.

    2017-11-01

    Full Text Available The article is aimed at researching practical aspects of the fleet-management development in Ukraine; relationships that arise in this process; elements that interact in the market. The preconditions of the companies’ transition to the use of this concept in the management of fleet, as well as the opportunities that arise in the enterprises using the principles of fleet-management were considered. The main advantages and disadvantages of the implementation of fleet-management in companies with the available fleet of various sizes were researched. The main features, current tendencies of the fleet-management development have been indicated, ways of attraction of automobile enterprises to the use of leasing have been suggested. Prospects for further researches in this direction will be consideration of possibility of attraction of more enterprises to the use of principles of fleet-management in their management routine. Further development of fleet management will provide enterprises with significant opportunities and the reserve funds that can be used for development.

  20. New heuristics for the fleet size and mix vehicle routing problem with time windows

    NARCIS (Netherlands)

    Dullaert, W.; Janssens, Gerrit K.; Sirensen, K.; Vernimmen, Bert

    2002-01-01

    In the Fleet Size and Mix Vehicle Routing Problem with Time Windows (FSMVRPTW) customers need to be serviced in their time windows at minimal costs by a heterogeneous fleet. In this paper new heuristics for the FSMVRPTW are developed. The performance of the heuristics is shown to be significantly

  1. 41 CFR 102-34.55 - Are there fleet average fuel economy standards we must meet?

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Are there fleet average... Management Federal Property Management Regulations System (Continued) FEDERAL MANAGEMENT REGULATION PERSONAL PROPERTY 34-MOTOR VEHICLE MANAGEMENT Obtaining Fuel Efficient Motor Vehicles § 102-34.55 Are there fleet...

  2. 33 CFR 165.1121 - Security Zone: Fleet Supply Center Industrial Pier, San Diego, CA.

    Science.gov (United States)

    2010-07-01

    ... Guard District § 165.1121 Security Zone: Fleet Supply Center Industrial Pier, San Diego, CA. (a... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Security Zone: Fleet Supply Center Industrial Pier, San Diego, CA. 165.1121 Section 165.1121 Navigation and Navigable Waters COAST...

  3. Clean Cities Plug-In Electric Vehicle Handbook for Fleet Managers

    Energy Technology Data Exchange (ETDEWEB)

    None

    2012-04-01

    Plug-in electric vehicles (PEVs) are entering the automobile market and are viable alternatives to conventional vehicles. This guide for fleet managers describes the basics of PEV technology, PEV benefits for fleets, how to select the right PEV, charging a PEV, and PEV maintenance.

  4. 77 FR 35862 - Safety Zone; Fleet Week Maritime Festival, Pier 66 Elliott Bay, Seattle, WA

    Science.gov (United States)

    2012-06-15

    ... DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 165 [Docket No. USCG-2010-0062] Safety Zone; Fleet Week Maritime Festival, Pier 66 Elliott Bay, Seattle, WA AGENCY: Coast Guard, DHS. ACTION: Notice of enforcement of regulation. SUMMARY: The Coast Guard will enforce the Fleet Week Maritime...

  5. 77 FR 18718 - Petroleum Reduction and Alternative Fuel Consumption Requirements for Federal Fleets

    Science.gov (United States)

    2012-03-28

    ... Petroleum Reduction and Alternative Fuel Consumption Requirements for Federal Fleets AGENCY: Office of...-required reduction in petroleum consumption and increase in alternative fuel consumption for Federal fleets... regulations for a statutorily-required reduction in petroleum consumption and increase in alternative fuel...

  6. Modelling the spatial behaviour of a tropical tuna purse seine fleet.

    Directory of Open Access Journals (Sweden)

    Tim K Davies

    Full Text Available Industrial tuna fisheries operate in the Indian, Atlantic and Pacific Oceans, but concerns over sustainability and environmental impacts of these fisheries have resulted in increased scrutiny of how they are managed. An important but often overlooked factor in the success or failure of tuna fisheries management is the behaviour of fishers and fishing fleets. Uncertainty in how a fishing fleet will respond to management or other influences can be reduced by anticipating fleet behaviour, although to date there has been little research directed at understanding and anticipating the human dimension of tuna fisheries. The aim of this study was to address gaps in knowledge of the behaviour of tuna fleets, using the Indian Ocean tropical tuna purse seine fishery as a case study. We use statistical modelling to examine the factors that influence the spatial behaviour of the purse seine fleet at broad spatiotemporal scales. This analysis reveals very high consistency between years in the use of seasonal fishing grounds by the fleet, as well as a forcing influence of biophysical ocean conditions on the distribution of fishing effort. These findings suggest strong inertia in the spatial behaviour of the fleet, which has important implications for predicting the response of the fleet to natural events or management measures (e.g., spatial closures.

  7. mRNA Display Selection of a High-Affinity, Modification-Specific Phospho-IκBα-Binding Fibronectin

    Science.gov (United States)

    Olson, C. Anders; Liao, Hsiang-I; Sun, Ren; Roberts, Richard W.

    2009-01-01

    The complexity of the human proteome is greatly expanded by post-translational modifications. New tools capable of recognizing these modifications in a sequence-specific fashion provide a route to purify these modified proteins, to alter protein trafficking, and to visualize signal transduction in real time. Here, we have evolved novel, modification-specific ligands that target phosphorylated IκBα. To do this, we employed mRNA display-based in vitro selection using a 30-trillion-member protein library based on the fibronectin type III domain. The selection yielded one fibronectin molecule, 10C17C25, that binds a phospho-IκBα peptide with Kd = 18 nM and is over 1000-fold specific compared to the nonphosphorylated peptide. 10C17C25 specifically recognizes endogenous phosphorylated IκBα from mammalian cell extract and stabilizes phospho-IκBα in vivo. We also incorporated 10C17C25 into a FRET indicator that detects IκB kinase (IKK) activity in vitro, demonstrating the utility of selecting designed adaptors for kinase activity sensors. PMID:18590330

  8. mRNA display selection of a high-affinity, modification-specific phospho-IkappaBalpha-binding fibronectin.

    Science.gov (United States)

    Olson, C Anders; Liao, Hsiang-I; Sun, Ren; Roberts, Richard W

    2008-08-15

    The complexity of the human proteome is greatly expanded by post-translational modifications. New tools capable of recognizing these modifications in a sequence-specific fashion provide a route to purify these modified proteins, to alter protein trafficking, and to visualize signal transduction in real time. Here, we have evolved novel, modification-specific ligands that target phosphorylated IkappaBalpha. To do this, we employed mRNA display-based in vitro selection using a 30-trillion-member protein library based on the fibronectin type III domain. The selection yielded one fibronectin molecule, 10C17C25, that binds a phospho-IkappaBalpha peptide with K d = 18 nM and is over 1000-fold specific compared to the nonphosphorylated peptide. 10C17C25 specifically recognizes endogenous phosphorylated IkappaBalpha from mammalian cell extract and stabilizes phospho-IkappaBalpha in vivo. We also incorporated 10C17C25 into a FRET indicator that detects IkappaB kinase (IKK) activity in vitro, demonstrating the utility of selecting designed adaptors for kinase activity sensors.

  9. The influence of phospho-tau on dendritic spines of cortical pyramidal neurons in patients with Alzheimer’s disease

    Science.gov (United States)

    Merino-Serrais, Paula; Benavides-Piccione, Ruth; Blazquez-Llorca, Lidia; Kastanauskaite, Asta; Rábano, Alberto; Avila, Jesús

    2013-01-01

    The dendritic spines on pyramidal cells represent the main postsynaptic elements of cortical excitatory synapses and they are fundamental structures in memory, learning and cognition. In the present study, we used intracellular injections of Lucifer yellow in fixed tissue to analyse over 19 500 dendritic spines that were completely reconstructed in three dimensions along the length of the basal dendrites of pyramidal neurons in the parahippocampal cortex and CA1 of patients with Alzheimer’s disease. Following intracellular injection, sections were immunostained for anti-Lucifer yellow and with tau monoclonal antibodies AT8 and PHF-1, which recognize tau phosphorylated at Ser202/Thr205 and at Ser396/404, respectively. We observed that the diffuse accumulation of phospho-tau in a putative pre-tangle state did not induce changes in the dendrites of pyramidal neurons, whereas the presence of tau aggregates forming intraneuronal neurofibrillary tangles was associated with progressive alteration of dendritic spines (loss of dendritic spines and changes in their morphology) and dendrite atrophy, depending on the degree of tangle development. Thus, the presence of phospho-tau in neurons does not necessarily mean that they suffer severe and irreversible effects as thought previously but rather, the characteristic cognitive impairment in Alzheimer’s disease is likely to depend on the relative number of neurons that have well developed tangles. PMID:23715095

  10. A multi-agent based intelligent configuration method for aircraft fleet maintenance personnel

    Directory of Open Access Journals (Sweden)

    Feng Qiang

    2014-04-01

    Full Text Available A multi-agent based fleet maintenance personnel configuration method is proposed to solve the mission oriented aircraft fleet maintenance personnel configuration problem. The maintenance process of an aircraft fleet is analyzed first. In the process each aircraft contains multiple parts, and different parts are repaired by personnel with different majors and levels. The factors and their relationship involved in the process of maintenance are analyzed and discussed. Then the whole maintenance process is described as a 3-layer multi-agent system (MAS model. A communication and reasoning strategy among the agents is put forward. A fleet maintenance personnel configuration algorithm is proposed based on contract net protocol (CNP. Finally, a fleet of 10 aircraft is studied for verification purposes. A mission type with 3 waves of continuous dispatch is imaged. Compared with the traditional methods that can just provide configuration results, the proposed method can provide optimal maintenance strategies as well.

  11. Închisoarea federală Oz - Un model de reprezentare mediatică a comunicării în grupurile din instituțiile închise

    Directory of Open Access Journals (Sweden)

    George ANGHELCEV

    2002-06-01

    Full Text Available The article seeks to explore the particular features of group communication networks and processes in the media-created environment of Oz, The Federal Prison. Specific characteristics of the OZ groups, their members, internal and external group pressures, distribution of status and group formation are analyzed by retorting to group communication theories and the pioneering work of American sociologist Ervin Goffman on prisons as total institutions. The analysis applies theorethical concepts to individual characters and actual contexts depicted throughout the series.

  12. 48 CFR 252.251-7001 - Use of Interagency Fleet Management System (IFMS) vehicles and related services.

    Science.gov (United States)

    2010-10-01

    ... Fleet Management System (IFMS) vehicles and related services. As prescribed in 251.205, use the following clause: Use of Interagency Fleet Management System (IFMS) Vehicles and Related Services (DEC 1991... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Use of Interagency Fleet...

  13. Live Cell Imaging Reveals Differential Modifications to Cytoplasmic Dynein Properties by Phospho- and Dephospho-mimic Mutations of the Intermediate Chain 2C S84

    Science.gov (United States)

    Blasier, Kiev R.; Humsi, Michael K.; Ha, Junghoon; Ross, Mitchell W.; Smiley, W. Russell; Inamdar, Nirja A.; Mitchell, David J.; Lo, Kevin W.-H.; Pfister, K. Kevin

    2014-01-01

    Cytoplasmic dynein is a multi-subunit motor protein responsible for intracellular cargo transport toward microtubule minus ends. There are multiple isoforms of the dynein intermediate chain (DYNC1I, IC) which is encoded by two genes. One way to regulate cytoplasmic dynein is by IC phosphorylation. The IC-2C isoform is expressed in all cells and the functional significance of phosphorylation on IC-2C serine 84 was investigated using live cell imaging of fluorescent protein-tagged wild type IC-2C (WT) and phospho- and dephospho-mimic mutant isoforms in axonal transport model systems. Both mutations modulated dynein functional properties. The dephospho-mimic mutant IC-2C S84A had greater co-localization with mitochondria than IC-2C wild-type (WT) or the phospho-mimic mutant IC-2C S84D. The dephospho-mimic mutant IC-2C S84A was also more likely to be motile than the phospho-mimic mutant IC-2C S84D or IC-2C WT. In contrast, the phospho-mimic mutant IC-2C S84D mutant was more likely to move in the retrograde direction than was the IC-2C S84A mutant. The phospho-mimic IC-2C S84D was also as likely as IC-2C WT to co-localize with mitochondria. Both the S84D phospho- and S84A, dephospho-mimic mutants were found to be capable of microtubule minus end directed (retrograde) movement in axons. They were also observed to be passively transported in the anterograde direction. These data suggest that the IC-2C S84 has a role in modulating dynein properties. PMID:24798412

  14. Optimal Routing for Heterogeneous Fixed Fleets of Multicompartment Vehicles

    Directory of Open Access Journals (Sweden)

    Qian Wang

    2014-01-01

    Full Text Available We present a metaheuristic called the reactive guided tabu search (RGTS to solve the heterogeneous fleet multicompartment vehicle routing problem (MCVRP, where a single vehicle is required for cotransporting multiple customer orders. MCVRP is commonly found in delivery of fashion apparel, petroleum distribution, food distribution, and waste collection. In searching the optimum solution of MCVRP, we need to handle a large amount of local optima in the solution spaces. To overcome this problem, we design three guiding mechanisms in which the search history is used to guide the search. The three mechanisms are experimentally demonstrated to be more efficient than the ones which only apply the known distance information. Armed with the guiding mechanisms and the well-known reactive mechanism, the RGTS can produce remarkable solutions in a reasonable computation time.

  15. First Battleships of the Coast Defense of Russian Emperor Fleet

    Directory of Open Access Journals (Sweden)

    Yuri F. Katorin

    2015-12-01

    Full Text Available In this article it is told about design and building in Russia of the first tower armored ships, are given their characteristics, are described design features, and also basic stages of service in the composition of Baltic fleet, the advantages and disadvantages in the projects are analyzed. The authors come to the conclusion that the first navigation of tower armored vehicles revealed many design flaws. Low-built monitors and two-turret armored boat had very poor seakeeping. However, for advertising purposes the ten monitors in 1865 have been sent to the coast of Sweden. During the operation it was determined the necessity of combining the reliable protection with sufficient seaworthiness. The solution to this problem is reflected in the subsequent types of armored ships – the tower frigates.

  16. A dynamic management of a public transportation fleet

    Directory of Open Access Journals (Sweden)

    Ireneusz Celiński

    2013-09-01

    Full Text Available Background: The present paper deals with the problems of a public transportation fleet management in public transportation operators. A management concept is proposed based on a real-time acquisition of parameters of public transportation passenger exchange. Methods: The relevant research utilised video materials documenting the processes of passenger exchange in public transportation. The proposed methodology is based on a dynamic real-time measurement of passenger streams. A characteristic feature of the measurement methodology applied is that the data is collected outside the vehicles, with a CCTV camera used per access point. Demand for the public transportation service are calculated using the image processing. Results: The derived demand characteristics allow not only an estimation of the magnitude of traffic streams in public transportation but also their qualitative description. Such an approach permits a flexible design of the transportation offer to adapt to the demand. This allows matching the timetables to the density functions describing the demand for public transportation within the space of transportation networks. In addition, based on the results of this type of research, a public transportation operator may despatch the vehicle base in a flexible way. For each run of a bus or tram fleet, basing on the registered passenger traffic streams, it is possible to rationally despatch the vehicles with suitable capacity. Conclusions: A system of this type is capable of determining the quality of work of the public transportation. With the ITS systems being introduced still more widely, the proposed methodology allows the design and implementation of dynamic timetables.

  17. Demonstration of fleet trucks fueled with PV hydrogen

    International Nuclear Information System (INIS)

    Provenzano, J.; Scott, P.B.; Zweig, R.

    1998-01-01

    The Clean Air Now (CAN) Solar Hydrogen Project has been installed at the Xerox Corporation, El Segundo, California site. Three Ford Ranger trucks have been converted to use hydrogen fuel. The ''stand- alone'' electrolyzer and hydrogen dispensing system is powered by a photovoltaic array with no connection to the power grid. A variable frequency DC/AC converter steps up the voltage to drive the 15 hp motor for the hydrogen compressor. Up to 400 standard cubic meters (SCM) of solar hydrogen is stored, and storage of up to 2300 SCM of commercial hydrogen is collocated. As the hydrogen storage is within 5km of Los Angeles International Airport, pilot operation of a hydrogen fuel cell bus for airport shuttle service has been demonstrated with fueling at the CAN facility. The truck engine conversions are bored to 2.91 displacement, use a Roots type supercharger and CVI (constant volume injection) fuel induction to allow performance similar to that of the gasoline powered truck. Truck fuel storage is done with carbon composite tanks at pressures up to 24.8 MPa (3600 psi). Two tanks are located just behind the driver's cab, and take up nearly half of the truck bed space. The truck highway range is approximately 140 miles. The engine operates in lean burn mode, with nil emissions of CO and HC. NO x emissions vary with load and rpm in the range from 10 to 100 ppm, yielding total emissions at a small fraction of the ULEV standard. Two Xerox fleet trucks have been converted, and one for the City of West Hollywood. The Clean Air Now Program demonstrates that hydrogen powered fleet development is an appropriate safe, and effective strategy for improvement of urban air quality. It further demonstrates that continued technological development and cost reduction will make such implementation competitive. (Author)

  18. Expanding the Telepresence Paradigm to the UNOLS Fleet

    Science.gov (United States)

    Coleman, D.; Scowcroft, G.

    2014-12-01

    The Inner Space Center (ISC) at the University of Rhode Island Graduate School of Oceanography (URI-GSO) has been at the forefront of developing the tools, techniques, and protocols for telepresence-enabled ocean science exploration and education programs. Working primarily with the Ocean Exploration Trust's E/V Nautilus and the NOAA Ship Okeanos Explorer, the ISC facility and staff have supported dozens of research cruises with significant shore-based support, while delivering related educational programming across the globe. Through a partnership with the University National Oceanographic Laboratory System (UNOLS), the ISC is broadening its reach and capabilities to serve vessels in the U.S. academic research fleet, managed by UNOLS. The ISC has developed and used a portable shipboard "mobile telepresence unit" (MTU) on several UNOLS ships to support projects led by ocean scientists that employed the telepresence paradigm as part of their research and outreach programs. Utilizing the ISC facilities provides opportunities for effective, successful broader impact activities and shore-based remote science connectivity. With new UNOLS ships coming online, including the R/V Sikuliaq, the R/V Neil Armstrong, and the R/V Sally Ride, in addition to future Regional Class Research Vessels (RCRVs), telepresence capability has become a technical requirement for a variety of reasons. Older vessels are being retrofit with this forward-looking technology, and URI's research vessel, the R/V Endeavor, has been recently configured with technology to support telepresence operations. This presentation will provide an overview of the future of telepresence technology, its use in ocean science research and education, and advantages for using this capability to support broader impact activities. In addition, ISC successes, challenges, and lessons learned in employing telepresence technologies and methodologies onboard the academic research fleet will be discussed.

  19. CCS Retrofit: Analysis of the Global Installed Power Plant Fleet

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2012-07-01

    Electricity generation from coal is still growing rapidly and energy scenarios from the IEA expect a possible increase from today’s 1 600 GW of coal-fired power plants to over 2 600 GW until 2035. This trend will increase the lock-in of carbon intensive electricity sources, while IEA assessments show that two-thirds of total abatement from all sectors should come from the power sector alone to support a least-cost abatement strategy. Since coal-fired power plants have a fairly long lifetime, and in order to meet climate constraints, there is a need either to apply CCS retrofit to some of today’s installed coal-fired power plants once the technology becomes available. Another option would be to retire some plants before the end of their lifetime. This working paper discusses criteria relevant to differentiating between the technical, cost-effective and realistic potential for CCS retrofit. The paper then discusses today’s coal-fired power plant fleet from a statistical perspective, by looking at age, size and the expected performance of today’s plant across several countries. The working paper also highlights the growing demand for applying CCS retrofitting to the coal-fired power plant fleet of the future. In doing so this paper aims at emphasising the need for policy makers, innovators and power plant operators to quickly complete the development of the CCS technology and to identify key countries where retrofit applications will have the biggest extent and impact.

  20. Binding of 5-phospho-D-arabinonohydroxamate and 5-phospho-D-arabinonate inhibitors to zinc phosphomannose isomerase from Candida albicans studied by polarizable molecular mechanics and quantum mechanics.

    Science.gov (United States)

    Roux, Celine; Gresh, Nohad; Perera, Lalith E; Piquemal, Jean-Philip; Salmon, Laurent

    2007-04-15

    Type I phosphomannose isomerase (PMI) is a Zn-dependent metalloenzyme involved in the isomerization of D-fructose 6-phosphate to D-mannose 6-phosphate. One of our laboratories has recently designed and synthesized 5-phospho-D-arabinonohydroxamate (5PAH), an inhibitor endowed with a nanomolar affinity for PMI (Roux et al., Biochemistry 2004, 43, 2926). By contrast, the 5-phospho-D-arabinonate (5PAA), in which the hydroxamate moiety is replaced by a carboxylate one, is devoid of inhibitory potency. Subsequent biochemical studies showed that in its PMI complex, 5PAH binds Zn(II) through its hydroxamate moiety rather than through its phosphate. These results have stimulated the present theoretical investigation in which we resort to the SIBFA polarizable molecular mechanics procedure to unravel the structural and energetical aspects of 5PAH and 5PAA binding to a 164-residue model of PMI. Consistent with the experimental results, our theoretical studies indicate that the complexation of PMI by 5PAH is much more favorable than by 5PAA, and that in the 5PAH complex, Zn(II) ligation by hydroxamate is much more favorable than by phosphate. Validations by parallel quantum-chemical computations on model of the recognition site extracted from the PMI-inhibitor complexes, and totaling up to 140 atoms, showed the values of the SIBFA intermolecular interaction energies in such models to be able to reproduce the quantum-chemistry ones with relative errors < 3%. On the basis of the PMI-5PAH SIBFA energy-minimized structure, we report the first hypothesis of a detailed view of the active site of the zinc PMI complexed to the high-energy intermediate analogue inhibitor, which allows us to identify active site residues likely involved in the proton transfer between the two adjacent carbons of the substrates. (c) 2007 Wiley Periodicals, Inc.

  1. Ozônio como agente fitossanitário na conservação pós-colheita da batata-baroa

    Directory of Open Access Journals (Sweden)

    Patrícia Helena Ribeiro

    2017-07-01

    Full Text Available Resumo Raízes de batata-baroa (Arracacia xanthorrhiza Bancroft foram imersas em água borbulhada com ozônio, para avaliar o controle de bactérias do gênero Erwinia. Além de análise visual das raízes, avaliaram-se a atividade das enzimas pectinolíticas pectinametilesterase e poligalacturonase, e as características físico-químicas (perda de massa, variação de cor, teores de açúcares e amido, durante o armazenamento. As raízes foram imersas em água ozonizada (1,52 mg L-1, por períodos de até 30 min. Em seguida, foram acondicionadas em câmara climática e avaliadas durante 10 dias. Não houve diferença visual aparente entre os tratamentos. Entretanto, raízes tratadas com ozônio por 30 min apresentaram menor atividade específica das enzimas pectinametilesterase. Houve efeito linear para açúcares solúveis totais e perda de massa, e efeito quadrático para açúcares redutores e não redutores, significativos apenas para o período de armazenamento. A imersão de raízes de batata-baroa em água borbulhada com ozônio por até 30 min não foi suficiente para o controle de bactérias do gênero Erwinia. Nas condições adotadas no trabalho, a ozonização não provoca alteração na perda de massa, no teor de amido, açúcares solúveis totais, açúcares redutores e açúcares não redutores, e na variação de cor das raízes de batata-baroa.

  2. An Optimization Method for Condition Based Maintenance of Aircraft Fleet Considering Prognostics Uncertainty

    Directory of Open Access Journals (Sweden)

    Qiang Feng

    2014-01-01

    Full Text Available An optimization method for condition based maintenance (CBM of aircraft fleet considering prognostics uncertainty is proposed. The CBM and dispatch process of aircraft fleet is analyzed first, and the alternative strategy sets for single aircraft are given. Then, the optimization problem of fleet CBM with lower maintenance cost and dispatch risk is translated to the combinatorial optimization problem of single aircraft strategy. Remain useful life (RUL distribution of the key line replaceable Module (LRM has been transformed into the failure probability of the aircraft and the fleet health status matrix is established. And the calculation method of the costs and risks for mission based on health status matrix and maintenance matrix is given. Further, an optimization method for fleet dispatch and CBM under acceptable risk is proposed based on an improved genetic algorithm. Finally, a fleet of 10 aircrafts is studied to verify the proposed method. The results shows that it could realize optimization and control of the aircraft fleet oriented to mission success.

  3. The molecular basis of FHA domain:phosphopeptide binding specificity and implications for phospho-dependent signaling mechanisms.

    Science.gov (United States)

    Durocher, D; Taylor, I A; Sarbassova, D; Haire, L F; Westcott, S L; Jackson, S P; Smerdon, S J; Yaffe, M B

    2000-11-01

    Forkhead-associated (FHA) domains are a class of ubiquitous signaling modules that appear to function through interactions with phosphorylated target molecules. We have used oriented peptide library screening to determine the optimal phosphopeptide binding motifs recognized by several FHA domains, including those within a number of DNA damage checkpoint kinases, and determined the X-ray structure of Rad53p-FHA1, in complex with a phospho-threonine peptide, at 1.6 A resolution. The structure reveals a striking similarity to the MH2 domains of Smad tumor suppressor proteins and reveals a mode of peptide binding that differs from SH2, 14-3-3, or PTB domain complexes. These results have important implications for DNA damage signaling and CHK2-dependent tumor suppression, and they indicate that FHA domains play important and unsuspected roles in S/T kinase signaling mechanisms in prokaryotes and eukaryotes.

  4. Preparation and optimization of ammonium phospho-molybdate reagent (FMA) (NH4)3[PMo12O40

    International Nuclear Information System (INIS)

    Cruz C, W.; Mallaupoma G, M.; Rodriguez C, G.

    1996-01-01

    The characteristics of low and medium level liquid radioactive waste produced in the Nuclear Research Center RACSO was identified, taking into account the philosophy of radiological safety. In liquid wastes, Cs-137 radionuclide could be present, which is important for radio-sanitary considerations. Its half life is 30 years. In the radioactive waste management, it is possible to separate Cs-137 by using a chemical treatment. One of the used chemical reagents is ammonium phospho-molybdate (FMA). The preparation method and the production optimization of FMA in the laboratory scale for its use as an economical reagent in the separation of Cs-137 radionuclide is shown in this paper. The objective is to get the higher decontamination factor and to reduce the volume containing the higher activity of the Cs-137 radionuclide. (authors). 4 refs., 2 tabs

  5. Novela Los dorados, de Sergio Muñoz: Jerarquización y exclusión en la sociedad costarricense

    Directory of Open Access Journals (Sweden)

    María Eugenia Rojas Rodríguez

    2012-11-01

    Full Text Available El texto de Sergio Muñoz devela una problemática socioeconómica del pueblo costarricense, la cual es palpable y visible. El aporte de su relato es que descarna a los personajes contando su historia de manera que supera la realidad con el diálogo que mantiene con el lector. La vida cotidiana de la novela transcurre en las coordenadas de la vida urbana marginal de los seres humanos excluidos. Es un relato de los de abajo que narra las miserias internas y externas de diversos personajes: drogadictos, alcohólicos, prostitutas, madres solteras, privados de libertad que, en algunos casos, ya han cumplido su condena; además, presenta a vendedores ambulantes, invasores de tierra, niños (as, hombres y mujeres que tratan de sobrevivir en este mundo tan desigual.

  6. UNC-ANALISTA: HACIA LA CAPTURA DE UN CORPUS DE REQUISITOS A PARTIR DE LA APLICACIÓN DEL EXPERIMENTO MAGO DE OZ

    Directory of Open Access Journals (Sweden)

    Carlos Mario Zapata

    2007-06-01

    Full Text Available La obtención de requisitos es una de las etapas más importantes en el proceso de desarrollo del software. Una buena comprensión de los requisitos puede conducir a mejores productos de software que satisfagan las necesidades de los interesados. Sin embargo, el proceso de captura de requisitos se torna difícil para el analista debido, en gran parte, al carácter presencial que tienen las reuniones que se realizan con tal fin y a la dificultad que presentan algunas personas para expresar sus ideas de forma clara. En este artículo se presenta UNC-Analista, una propuesta de experimento Mago de Oz enfocado al diseño de un sistema de diálogo controlado que posibilite la labor del analista durante el proceso de obtención de requisitos. Con este sistema será posible capturar un corpus de requisitos, que servirá como base para la construcción futura de un sistema automático para la obtención de requisitos.Requirements elicitation is one of the most important phases in software development process. A good requirements understanding can lead to better software products, achieving satisfaction of stakeholder needs. However, requirements-capture process is sometimes difficult for analysts, because of the face-to-face character of the meetings required for it and because of difficulties of people for expressing clearly their ideas. In this paper we present UNC-Analista, a proposal for Wizard-of-Oz experiment focused on the design of a dialogue-controlled system for helping the analyst labor in the requirements elicitation process. With this system will be possible to capture a requirements corpus, for leading a future development of an automatic requirements elicitation system.

  7. Unbiased Selective Isolation of Protein N-Terminal Peptides from Complex Proteome Samples Using Phospho Tagging PTAG) and TiO2-based Depletion

    NARCIS (Netherlands)

    Mommen, G.P.M.; Waterbeemd, van de B.; Meiring, H.D.; Kersten, G.; Heck, A.J.R.; Jong, de A.P.J.M.

    2012-01-01

    A positional proteomics strategy for global N-proteome analysis is presented based on phospho tagging (PTAG) of internal peptides followed by depletion by titanium dioxide (TiO2) affinity chromatography. Therefore, N-terminal and lysine amino groups are initially completely dimethylated with

  8. Interaction between the thyroid hormone receptor and co-factors on the promoter of the gene encoding phospho enol pyruvate carboxykinase

    NARCIS (Netherlands)

    Schmidt, E. D.; van Beeren, M.; Glass, C. K.; Wiersinga, W. M.; Lamers, W. H.

    1993-01-01

    Using transient transfection studies we localized a thyroid hormone-responsive element on the promoter of the rat phospho-enol pyruvate carboxykinase gene between 355 and 174 bp upstream of the transcription start site. DNAse 1 footprinting analysis within this region showed that a 28 bp fragment at

  9. Use of long-term stable CsPbBr3 perovskite quantum dots in phospho-silicate glass for highly efficient white LEDs.

    Science.gov (United States)

    Di, Xiaoxuan; Hu, Zemin; Jiang, Jutao; He, Meiling; Zhou, Lei; Xiang, Weidong; Liang, Xiaojuan

    2017-10-05

    We report the synthesis of CsPbBr 3 QDs with great stability and high quantum yield in phospho-silicate glass, which was fabricated by using a heat-treatment approach, for white light emitting devices. QD glasses exhibited excellent photo- and thermal stability, and significantly prolonged the lifetime of light emitters under ambient air conditions.

  10. Decomposition kinetics of gaseous ozone in peanuts Cinética da decomposição do gás ozônio em amendoim

    Directory of Open Access Journals (Sweden)

    Ernandes R. de Alencar

    2011-10-01

    Full Text Available This study was conducted to evaluate the decomposition kinetics of gaseous ozone in peanut grains. This evaluation was made with 1-kg peanut samples, moisture contents being 7.1 and 10.5% wet basis (w.b., placed in 3-liter glass containers. The peanut grains were ozonated at the concentration of 450 µg L-1, at 25 and 35 ºC, with gas flow rates of 1.0 and 3.0 L min-1. Time of saturation was determined by quantifying the residual concentration of ozone after the gas passed through the grains to constant mass. The decomposition kinetics of ozone was evaluated after the grain mass was ozone-saturated. For the peanut grains whose moisture content was 7.1% (w.b., at 25 and 35ºC and with flow rates of 1.0 and 3.0 L min-1, the values obtained for time of saturation of gaseous ozone ranged between 173 and 192 min; the concentration of saturation was approximately 260 µg L-1. For the grains whose moisture content was 10.5% (w.b., a higher residual concentration of gaseous ozone was obtained at 25 ºC, that of 190 µg L-1. As regards the half-life of ozone, the highest value obtained was equivalent to 7.7 min for grains ozonated at 25 ºC, while for those with moisture content of 10.5% at 35 ºC, half-life was 3.2 min. In the process of ozone decomposition in peanut grains, temperature was concluded to be the key factor. An increase of 10 ºC in the temperature of the grains results in a decrease of at least 43% in the half-life of ozone.Este trabalho foi desenvolvido com o objetivo de avaliar a cinética de decomposição do ozônio em grãos de amendoim. Para avaliar a cinética de decomposição do gás, utilizaram-se amostras de 1 kg de amendoim, com teores de água de 7,1 e 10,5% base úmida (b.u., acondicionadas em recipientes de vidro com capacidade de 3 L. Os grãos de amendoim foram ozonizados na concentração de 450 µg L-1, nas temperaturas de 25 e 35 ºC, e vazões do gás de 1,0 e 3,0 L min-1. Determinou-se o tempo de satura

  11. Mission Engineering of a Rapid Cycle Spacecraft Logistics Fleet

    Science.gov (United States)

    Holladay, Jon; McClendon, Randy (Technical Monitor)

    2002-01-01

    The requirement for logistics re-supply of the International Space Station has provided a unique opportunity for engineering the implementation of NASA's first dedicated pressurized logistics carrier fleet. The NASA fleet is comprised of three Multi-Purpose Logistics Modules (MPLM) provided to NASA by the Italian Space Agency in return for operations time aboard the International Space Station. Marshall Space Flight Center was responsible for oversight of the hardware development from preliminary design through acceptance of the third flight unit, and currently manages the flight hardware sustaining engineering and mission engineering activities. The actual MPLM Mission began prior to NASA acceptance of the first flight unit in 1999 and will continue until the de-commission of the International Space Station that is planned for 20xx. Mission engineering of the MPLM program requires a broad focus on three distinct yet inter-related operations processes: pre-flight, flight operations, and post-flight turn-around. Within each primary area exist several complex subsets of distinct and inter-related activities. Pre-flight processing includes the evaluation of carrier hardware readiness for space flight. This includes integration of payload into the carrier, integration of the carrier into the launch vehicle, and integration of the carrier onto the orbital platform. Flight operations include the actual carrier operations during flight and any required real-time ground support. Post-flight processing includes de-integration of the carrier hardware from the launch vehicle, de-integration of the payload, and preparation for returning the carrier to pre-flight staging. Typical space operations are engineered around the requirements and objectives of a dedicated mission on a dedicated operational platform (i.e. Launch or Orbiting Vehicle). The MPLM, however, has expanded this envelope by requiring operations with both vehicles during flight as well as pre-launch and post

  12. Real and alleged hazard of radioactive contamination of seas caused by activities of Russian nuclear fleets

    International Nuclear Information System (INIS)

    Lavkovsky, S.A.

    1999-01-01

    The current paper addresses the assessment results of the degree of danger caused by radioactive contamination of seas by wastes from activities of nuclear fleets compared to the results obtained in other works, specifically, in the IASAP program

  13. Maritime Security Fleet: Factors to Consider before Deciding to Select Participants Competitively

    National Research Council Canada - National Science Library

    1997-01-01

    The Maritime Security Act established the Maritime Security Fleet (MSF) program, authorizing MARAD to enter into agreements with maritime shipping companies to provide DOD access to commercial vessels operating under U.S...

  14. Vehicle attributes constraining present electric car applicability in the fleet market

    Energy Technology Data Exchange (ETDEWEB)

    Wagner, J R

    1979-12-01

    One strategy for reducing petroleum imports is to use electric cars in place of conventional vehicles. This paper examines obstacles which electric cars are likely to encounter in attempting to penetrate a key segment of the passenger car market, namely, the fleet market. A fleet is here defined as a group of cars operated by a corporation or a government agency. The primary data source is a questionnaire that was distributed to fleet operators by the Bobit Publishing Company in the summer of 1977. Six sectors of the fleet market were sampled: police, state and local government, utilities, taxi, rental, and business. The questionnaire was specifically designed to uncover factors limiting market penetration of unconventional vehicles, although no attempt was made to determine price elasticities. Emphasis is on vehicle attributes that are readily quantifiable and relatively projectable, including seating capacity, range, battery recharging characteristics, availability of power options, and ability to use interstate highways.

  15. Fleet-Car Market PENetration Simulator: CPEN user's guide

    Energy Technology Data Exchange (ETDEWEB)

    Weil, R.

    1980-08-01

    The purpose of this manual is to assist prospective users in the understanding and execution of Fleet-Car Market PENetration Simulator (CPEN). CPEN is an interactive FORTRAN program whose purpose is to produce estimates of fleet-market-penetration rates of alternative passenger cars that can be described in terms of specific physical and economic attributes. The data were derived from questionnaires distributed to fleet operators affiliated with National Association of Fleet Administrators (NAFA). Besides the NAFA data, CPEN uses 48 variables that are interactively inserted. Complete data-input descriptions are included in the manual along with algorithm and application flowcharts. Examples of complete successful simulator runs are included for alternative program paths. A listing of the computer program and a glossary for CPEN are included.

  16. AVTA Federal Fleet PEV Readiness Data Logging and Characterization Study: Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Schey, Stephen [Idaho National Laboratory (INL), Idaho Falls, ID (United States); Francfort, Jim [Idaho National Laboratory (INL), Idaho Falls, ID (United States)

    2015-06-01

    Collect and evaluate data on federal fleet operations as part of the Advanced Vehicle Testing Activity’s Federal Fleet Vehicle Data Logging and Characterization Study. The Advanced Vehicle Testing Activity study seeks to collect and evaluate data to validate the utilization of advanced plug-in electric vehicle (PEV) transportation. This report summarizes the fleets studied to identify daily operational characteristics of select vehicles and report findings on vehicle and mission characterizations to support the successful introduction of PEVs into the agencies’ fleets. Individual observations of these selected vehicles provide the basis for recommendations related to electric vehicle adoption and whether a battery electric vehicle or plug-in hybrid electric vehicle (collectively referred to as PEVs) can fulfill the mission requirements.

  17. Optimal Scheduling and Operating Target (OPTAR) Cost Model for Aircraft Carriers in the Fleet Response Plan

    National Research Council Canada - National Science Library

    York, Michael A

    2008-01-01

    .... This capability is developed through the Fleet Readiness Training Plan (FRTP) where the Navy's carriers are scheduled in staggered 32-month cycles consisting of four phases of progressive readiness levels...

  18. A Study of Fleet Surgical Teams Readiness Posture in Amphibious Readiness Groups

    National Research Council Canada - National Science Library

    Tennyson, Ruby

    2000-01-01

    This thesis describes and evaluates Fleet Surgical Teams (FSTs). It examines how Navy Medicine adapted FSTs to changing support requirements associated with the Total Health Care Support Readiness Requirement (THCSRR...

  19. Satisfaction of the Automotive Fleet Fuel Demand and Its Impact on the Oil Refining Industry

    Science.gov (United States)

    1980-12-01

    Because virtually all transportation fuels are based on petroleum, it is essential to include petroleum refining in any assessment of potential changes in the transportation system. A number of changes in the automotive fleet have been proposed to im...

  20. PROBLEMS OF ROUTE NETWORK AND AIRCRAFT FLEET OPTIMIZATION AS A SPECIFIC TASK OF AIRLINE STRATEGIC PLANNING

    Directory of Open Access Journals (Sweden)

    M. A. Karakuts

    2015-01-01

    Full Text Available The basic problems of route network and aircraft fleet optimization and its role in airline strategic planning are considered. Measures to improve the methods of its implementation are proposed.

  1. The design of a comprehensive microsimulator of household vehicle fleet composition, utilization, and evolution.

    Science.gov (United States)

    2012-01-01

    The report describes a comprehensive vehicle fleet composition, utilization, and evolution : simulator that can be used to forecast household vehicle ownership and mileage by type of : vehicle over time. The components of the simulator are developed ...

  2. Idle reduction assessment for the New York State Department of Transportation region 4 fleet.

    Science.gov (United States)

    2015-03-01

    Energetics Incorporated conducted a study to evaluate the operational, economic, and environmental impacts of advanced technologies to reduce idling in : the New York State Department of Transportation (NYSDOT) Region 4 fleet without compromising fun...

  3. Decision support with respect to facility location and fleet composition for FoodBank Cape Town

    CSIR Research Space (South Africa)

    Lanz, EJ

    2011-09-01

    Full Text Available Fleet Composition Current & Future Work FoodBank Cape Town First in South Africa Launched on 2 March 2009 Warehouse located in Philippi East [1] Majority of food sourced from DCs and retail sector Distributing to approximately 200 agencies... & Future Work Figure: Sourcing & distributing ow diagram EJ Lanz 40th Annual ORSSA Conference 11 of 36 Background Project Focus Demand & Candidate Sites Facility Location Problems Vehicle Fleet Composition Current & Future Work Data Demand Site...

  4. A New Formulation for the Combined Maritime Fleet Deployment and Inventory Management Problem

    OpenAIRE

    Dong, Bo; Bektas, Tolga; Chandra, Saurabh; Christiansen, Marielle; Fagerholt, Kjetil

    2017-01-01

    This paper addresses the fleet deployment problem and in particular the treatment of inventory in the maritime case. A new model based on time-continuous formulation for the combined maritime fleet deployment and inventory management problem in Roll-on Roll-off shipping is presented. Tests based on realistic data from the Ro-Ro business show that the model yields good solutions to the combined problem within reasonable time.

  5. An evaluation and implementation study of the Zenith 120 microcomputer in West Coast Fleet Commands

    OpenAIRE

    Barnett, George A.

    1985-01-01

    Approved for public release; distribution is unlimited Austere funding and the increased administrative burdens being placed on fleet units will ultimately take their toll on performance, morale and combat readiness. Applying low cost microcomputer technology presents one method to alleviate many of these burdens. However, the current implementation process being used is not sufficient to meet the needs of the Fleet. Empirical evidence indicates that expenditures on computers are not ac...

  6. An Intelligent Fleet Condition-Based Maintenance Decision Making Method Based on Multi-Agent

    OpenAIRE

    Bo Sun; Qiang Feng; Songjie Li

    2012-01-01

    According to the demand for condition-based maintenance online decision making among a mission oriented fleet, an intelligent maintenance decision making method based on Multi-agent and heuristic rules is proposed. The process of condition-based maintenance within an aircraft fleet (each containing one or more Line Replaceable Modules) based on multiple maintenance thresholds is analyzed. Then the process is abstracted into a Multi-Agent Model, a 2-layer model structure containing host negoti...

  7. Work environment and safety climate in the Swedish merchant fleet.

    Science.gov (United States)

    Forsell, Karl; Eriksson, Helena; Järvholm, Bengt; Lundh, Monica; Andersson, Eva; Nilsson, Ralph

    2017-02-01

    To get knowledge of the work environment for seafarers sailing under the Swedish flag, in terms of safety climate, ergonomical, chemical and psychosocial exposures, and the seafarers self-rated health and work ability. A Web-based questionnaire was sent to all seafarers with a personal e-mail address in the Swedish Maritime Registry (N = 5608). Comparisons were made mainly within the study population, using Student's t test, prevalence odds ratios and logistic regressions with 95% confidence intervals. The response rate was 35% (N = 1972; 10% women, 90% men), with 61% of the respondents working on deck, 31% in the engine room and 7% in the catering/service department (1% not classifiable). Strain on neck, arm or back and heavy lifting were associated with female gender (p = 0.0001) and younger age (below or above 30 years of age, p harassment or bullying during last year of service. Noise, risk of accidents, hand/arm and whole-body vibrations and psychosocial factors such as harassment were commonly reported work environment problems among seafarers within the Swedish merchant fleet.

  8. Managing fleet capacity effectively under second-hand market redistribution.

    Science.gov (United States)

    Quillérou, Emmanuelle; Roudaut, Nolwenn; Guyader, Olivier

    2013-09-01

    Fishing capacity management policies have been traditionally implemented at national level with national targets for capacity reduction. More recently, capacity management policies have increasingly targeted specific fisheries. French fisheries spatially vary along the French coastline and are associated to specific regions. Capacity management policies, however, ignore the capital mobility associated with second-hand vessel trade between regions. This is not an issue for national policies but could limit the effectiveness of regional capacity management policies. A gravity model and a random-effect Poisson regression model are used to analyze the determinants and spatial extent of the second-hand market in France. This study is based on panel data from the French Atlantic Ocean between 1992 and 2009. The trade flows between trading partners is found to increase with their sizes and to be spatially concentrated. Despite the low trade flows between regions, a net impact analysis shows that fishing capacity is redistributed by the second-hand market to regions on the Channel and Aquitaine from central regions. National capacity management policies (constructions/destructions) have induced a net decrease in regional fleet capacity with varying magnitude across regions. Unless there is a change of policy instruments or their scale of implementation, the operation of the second-hand market decreases the effectiveness of regional capacity management policies in regions on the Channel and Aquitaine.

  9. Liner Shipping Fleet Deployment with Sustainable Collaborative Transportation

    Directory of Open Access Journals (Sweden)

    Gang Du

    2016-02-01

    Full Text Available Facing sharp competition in the market for shipping companies, it is necessary to make reasonable and efficient decisions to optimize the container shipping line network so as to improve the shipping efficiency and reduce the transportation cost, as well as to realize the transportation sustainability. Therefore, the liner ship fleet deployment problem with collaborative transportation is proposed in this paper. This problem is formulated as a mixed-integer linear programming model that takes collaborative transportation into consideration. The model includes fixed cost, variable cost, berth cost, transport cost, penalty, compensation cost, and so on. To achieve the sustainable development of collaborative transportation, the shipping companies could make a selection between the internal routes and the external routes to serve each task by comparing the distance between the above routes. A real Asia-Europe-Oceania numerical experiment shows that the proposed sustainable collaborative transportation model can be efficiently solved by C++ calling ILOG CPLEX. Results demonstrate that the optimized shipping line network with sustainable collaborative transportation can improve the service efficiency, as well as the service level of shipping companies.

  10. Cooperation is fleeting in the world of transposable elements.

    Directory of Open Access Journals (Sweden)

    Andreas Wagner

    2006-12-01

    Full Text Available Composite transposons are key vehicles for the worldwide spreading of genes that allow bacteria to survive toxic compounds. Composite transposons consist of two smaller transposable elements called insertion sequences (ISs, which flank the genes that permit such survival. Each IS in a composite transposon can either transpose alone, selfishly, or it can transpose cooperatively, jointly with the other IS. Cooperative transposition can enhance an IS's chance of survival, but it also carries the risk of transposon destruction. I use game theory to show that the conditions under which cooperative transposition is an evolutionarily stable strategy (ESS are not biologically realistic. I then analyze the distribution of thousands of ISs in more than 200 bacterial genomes to test the following prediction of the game-theoretical model: if cooperative transposition was an ESS, then the closely spaced ISs that characterize composite transposons should be more abundant in genomes than expected by chance. The data show that this is not the case. Cooperativity can only be maintained in a transitional, far-from-equilibrium state shortly after a selection pressure first arises. This is the case in the spreading of antibiotic resistance, where we are witnessing a fleeting moment in evolution, a moment in which cooperation among selfish DNA molecules has provided a means of survival. Because such cooperation does not pay in the long run, the vehicles of such survival will eventually disappear again. My analysis demonstrates that game theory can help explain behavioral strategies even for mobile DNA.

  11. More fleet adjustments seen needed for drilling industry

    International Nuclear Information System (INIS)

    Lundy, K.

    1992-01-01

    The Canadian oil and gas industry has been in a mode of restructuring since the late 1980s as it became increasingly apparent that the natural gas boom which was to replace activity previously generated by high oil process was not going to materialize. This paper reports that the production companies operating in Canada are concentrating on rationalization of land holdings and corporate downsizing and realignment rather than exploration and development of petroleum resources. Not surprisingly, this has resulted in a substantial downturn of the contract drilling and well servicing sectors in both western Canada, where the majority (95%) of the industry activity takes place, and in the frontier and East Coast offshore arenas. The persistently low levels of utilization throughout this period and prior to the Organization of Petroleum Exporting Countries price shocks in the '70s illustrate that the high levels achieved in the late '70s and early '80s were an aberration. The rig fleet, which was expanded to meet this abnormally high demand, must continue to rationalize if profitability is to return on an on an industrywide basis

  12. Implications of energy use for fishing fleet-Taiwan example

    International Nuclear Information System (INIS)

    Hua Jian; Wu Yihusan

    2011-01-01

    Commercial fisheries rely heavily on fossil fuel combustion and contribute heavily to the emission of atmospheric pollutants and greenhouse gases. Propulsion output of fishing vessels has continually increased from 30 kW in 1959 to nearly 320 kW in 2000, indicating that the Taiwanese fishing fleet tended to voyage farther and faster, and to adjust for the heavier loads demanded by more powerful fishing gear. Daily emissions from Taiwanese fishing vessels were estimated using output method. The marine fishery is unlikely to grow in the future as the government is implementing measures to ensure the development of sustainable fishing practices. There has been a rising trend in pollution to production ratios during the study period between 1959 and 2008. The ratio increased by 47% in the first decade, followed by fluctuations within the range of 50%-58% for the remainder of the statistical period. There is a need to investigate the possibility of reductions in all categories of fishing with regard to energy use and emissions through the subsidization of fishing vessels to encourage operators to switch to more energy efficient equipment and cleaner fuels. - Highlights: → Daily emissions from Taiwanese fishing vessels were estimated using output method. → We reported a steady increase in pollution to production ratio in Taiwan fishing industry. → We urged government and industry work together to ensure a transition to low carbon fishing.

  13. The Insufficient Issues In Development Of Vietnamese Ship Fleet

    Directory of Open Access Journals (Sweden)

    Xuan Nam Chu

    2017-08-01

    Full Text Available According to the Vietnam Maritime Administration in 2015 the total volume of transport carried was about 118.7 million tons an increase of 9.5 compared to 2014 - the biggest increase in recent years. However the fleet of Vietnam has taken only 27.8 of total cargo through the port more than 70 of the market share of foreign shipping. By the end of April 2017 the Seal of Memorandum of Understanding PSC of the Asia-Pacific Region Tokyo-MOU comprising 20 members Australia Canada Chile China Fiji Hong Kong China Indonesia Japan South Korea Malaysia Marshall Islands New Zealand Papua New Guinea Peru Philippines Russian Federation Singapore Thailand Vanuatu and Vietnam presented the results of this organization in 2016. Accordingly in 2016 members of Tokyo-MOU conducted 31678 PSC inspections of 17503 flag-nautical vessels of 101 countries to regional ports. In 31678 PSC inspections 18943 defect detection ships affected safety security maritime labor conditions environmental pollution prevention and 1090 of flagged vessels from 69 countries. Kept due to serious defects the average retention of the entire region was 3.44 down from 3.67 in 2015 and 3.96 in 2014.

  14. B612 plans asteroid hunt with fleet of small satellites

    Science.gov (United States)

    Mann, Adam

    2018-05-01

    Last week, an asteroid the size of Egypt's Great Pyramid of Giza whizzed by Earth, missing it by half the distance to the moon. The concern that we may one day not be so lucky has long preoccupied the B612 Foundation, a private organization in Mill Valley, California, dedicated to finding asteroids that cross Earth's orbit and could devastate humanity. B612 itself had a near-death experience 3 years ago, when its bold plans for an asteroid-hunting space telescope fell apart. But now, its ambitions are rising again with a new technique for finding menacing objects. On 10 May, B612 announced a partnership with York Space Systems to investigate building a fleet of small asteroid hunters. For many years, B612 aimed to build and launch a much larger craft, Sentinel, a $450 million space telescope with a 50-centimeter mirror. But fundraising stalled and, in 2015, NASA ended an agreement to help B612 because it wasn't meeting mileposts, essentially killing the telescope. Now, B612 has developed a new technique, called synthetic tracking, that can produce similar results at a far lower cost with small space telescopes. Ed Lu, B612 co-founder and CEO, expects the first telescope to cost about $10 million and believes a full constellation "would be a factor of many, many cheaper" than Sentinel.

  15. Measures to reduce car-fleet consumption - Estimation of effects

    International Nuclear Information System (INIS)

    Iten, R.; Hammer, S.; Keller, M.; Schmidt, N.; Sammer, K.; Wuestenhagen, R.

    2005-09-01

    This comprehensive report for the Swiss Federal Office of Energy (SFOE) takes a look at the results of a study that estimated the effects of measures that were to be taken in order to reduce the fuel consumption of fleets of vehicles as part of the SwissEnergy programme. The research reported on aimed to estimate the effects of the Energy Label on energy consumption and research concerning the results to be expected from the introduction of a bonus-malus system. Questions reviewed include the effect of fuel consumption data on making decisions concerning which vehicle to purchase, the effects of the Energy Label on consumption, the awareness of other appropriate information sources, the possible effects of a bonus-malus system and how the effectiveness of the Energy Label could be improved. The answers and results obtained are reviewed and commented on. Finally, an overall appraisal of the situation is presented and recommendations for increasing the effectiveness of the Energy Label are made

  16. Web Map Apps using NASA's Earth Observing Fleet

    Science.gov (United States)

    Boller, R.; Baynes, K.; Pressley, N.; Thompson, C.; Cechini, M.; Schmaltz, J.; Alarcon, C.; De Cesare, C.; Gunnoe, T.; Wong, M.; hide

    2016-01-01

    Through the miracle of open web mapping services for satellite imagery, a garden of new applications has sprouted to monitor the planet across a variety of domains. The Global Imagery Browse Services (GIBS) provide free and open access to full resolution imagery captured by NASAs Earth observing fleet. Spanning 15+ years and running through as recently as a few hours ago, GIBS aims to provide a general-purpose window into NASA's vast archive of the planet. While the vast nature of this archive can be daunting, many domain-specific applications have been built to meet the needs of their respective communities. This presentation will demonstrate a diverse set of these new applications which can take planetarium visitors into (virtual) orbit, guide fire resource managers to hotspots, help anglers find their next catch, illustrate global air quality patterns to local regulators, and even spur a friendly competition to find clouds which are shaped the most like cats. We hope this garden will continue to grow and will illustrate upcoming upgrades to GIBS which may open new pathways for development. data visualization, web services, open access

  17. ENVIRONMENTAL IMPACT, SUSTAINABILITY AND GROWTH DISORDER FLEET OF MOTOR VEHICLES OF THE STATE OF CEARÁ

    Directory of Open Access Journals (Sweden)

    Marcus Vinicius de Oliveira Brasil

    2014-05-01

    Full Text Available Traffic jams, parking difficulties, noise horns, especially stress and the increment of air pollution by greenhouse gas emissions by the growing fleet of motor vehicles in Brazilian capitals, then, the question is: what are the possible impacts that the growing fleet of motor vehicles of the State of Ceará may cause to the environment? With the general aim of this study: to analyze the growing fleet of vehicles in the State of Ceará and its possible environmental impacts. And yet with the following specific objectives: to analyze the determinants of growth in vehicle fleet of the state of Ceará, by applying the statistical technique of Multiple Regression; discuss the relationship between economic development and environmental mitigation measures related to the growth fleet of automotive vehicles. This is a literature review, using secondary data that was applied multiple regression analysis. It was made a data analysis about the period between 1980 to 2009. Thiswork serves asawarningas theuncontrolled growthof the fleet ofvehiclesleads to anincrease in pollutionby the emission oftoxic gases, whosedirect consequence isthe destructionof the ozone layerthat protectsthe earth’s atmosphere from the exposure of UV irradiation.

  18. A systems approach to improving fleet policy compliance within the US Federal Government

    Energy Technology Data Exchange (ETDEWEB)

    Deason, Kristin S. [The George Washington University, 1776 G St. NW, Washington, DC 20052 (United States); Jefferson, Theresa [Virginia Polytechnic Institute and State University, 1101 King St, Suite 610 Alexandria, VA 22314 (United States)

    2010-06-15

    To reduce dependence on foreign sources of energy, address climate change, and improve environmental quality, the US government has established a goal of reducing petroleum fuel use in its federal agencies. To this end, the government requires its agencies to purchase alternative fuel vehicles, use alternative fuel, and adopt other strategies to reduce petroleum consumption. Compliance with these requirements, while important, creates challenges for federal fleet managers who oversee large, geographically dispersed fleets. In this study, a group of 25 experienced federal fleet managers participated in a pilot study using a structured methodology for developing strategies to comply with fleet requirements while using agency resources as efficiently as possible. Multi-criteria decision making (MCDM) methods were used to identify and quantify agency priorities in combination with a linear programming model to optimize the purchase of fleet vehicles. The method was successful in quantifying tradeoffs and decreasing the amount of time required to develop fleet management strategies. As such, it is recommended to federal agencies as a standard tool for the development of these strategies in the future. (author)

  19. A systems approach to improving fleet policy compliance within the US Federal Government

    International Nuclear Information System (INIS)

    Deason, Kristin S.; Jefferson, Theresa

    2010-01-01

    To reduce dependence on foreign sources of energy, address climate change, and improve environmental quality, the US government has established a goal of reducing petroleum fuel use in its federal agencies. To this end, the government requires its agencies to purchase alternative fuel vehicles, use alternative fuel, and adopt other strategies to reduce petroleum consumption. Compliance with these requirements, while important, creates challenges for federal fleet managers who oversee large, geographically dispersed fleets. In this study, a group of 25 experienced federal fleet managers participated in a pilot study using a structured methodology for developing strategies to comply with fleet requirements while using agency resources as efficiently as possible. Multi-criteria decision making (MCDM) methods were used to identify and quantify agency priorities in combination with a linear programming model to optimize the purchase of fleet vehicles. The method was successful in quantifying tradeoffs and decreasing the amount of time required to develop fleet management strategies. As such, it is recommended to federal agencies as a standard tool for the development of these strategies in the future. (author)

  20. Software Application for Remote Monitoring of Fleets Based on Geographic Information Systems Using Open Source Technologies.

    Directory of Open Access Journals (Sweden)

    Jesse Daniel Cano

    2012-11-01

    Full Text Available Controlling a fleet usually implies to establish means of control of vehicles, to collect the data associated with the routes taken by these vehicles, to interpret and evaluate the meaning of the collected data and to make the appropriate decisions to improve the efficiency in the use of vehicles in an organization. The implementation of this process of fleet management is mainly performed manually and the solutions available on the market are costly because of the payments for licenses, it is also necessary that the people monitoring the fleets are geographically close to them. This paper aims to answer the following questions: How to reduce errors in the management of information resulting from the fleet management process? How to reduce the cost of remote fleet monitoring? To obtain the solution, we propose the use of GPS devices in each vehicle, the GPS device’s information is captured and consistently stored in a data base, then the information is consulted, analyzed and represented on a map. The result is a software application that allows users have fast and reliable information that will enable them to take the necessary decisions in the vehicle fleet they are trying to control at a low cost.

  1. Evaluation and comparison of alternative fleet-level selective maintenance models

    International Nuclear Information System (INIS)

    Schneider, Kellie; Richard Cassady, C.

    2015-01-01

    Fleet-level selective maintenance refers to the process of identifying the subset of maintenance actions to perform on a fleet of repairable systems when the maintenance resources allocated to the fleet are insufficient for performing all desirable maintenance actions. The original fleet-level selective maintenance model is designed to maximize the probability that all missions in a future set are completed successfully. We extend this model in several ways. First, we consider a cost-based optimization model and show that a special case of this model maximizes the expected value of the number of successful missions in the future set. We also consider the situation in which one or more of the future missions may be canceled. These models and the original fleet-level selective maintenance optimization models are nonlinear. Therefore, we also consider an alternative model in which the objective function can be linearized. We show that the alternative model is a good approximation to the other models. - Highlights: • Investigate nonlinear fleet-level selective maintenance optimization models. • A cost based model is used to maximize the expected number of successful missions. • Another model is allowed to cancel missions if reliability is sufficiently low. • An alternative model has an objective function that can be linearized. • We show that the alternative model is a good approximation to the other models

  2. Layup practices at EDF PWR fleet and future developments

    International Nuclear Information System (INIS)

    Ramos, Olga Alos; Pablo, Sergio De Maria; Wintergerst, Matthieu

    2014-01-01

    A proper lay-up can only be achieved by plant specific lay-up procedures. The layup control practices are necessary to prevent corrosion damage during shutdown and are an important part of the life time extension of a nuclear power plant. EDF operational experience in this area confirms that damage to plant equipment from improper layup procedures is a cause of reduced plant reliability and availability and can increase the corrosion products ingress into the SG during start-up. The plant preservation strategy applied across the EDF fleet is based on the following principles: Anticipated time frame of the required layup: short-term outages can become long-term outages, and can necessitate the use of unplanned preservation techniques. Dry layup for a long-term outage of the condensate/feedwater system: condenser vacuum assisted draining at high temperature, estimation of air requirements (flow rate, humidity...) to guarantee the optimum conditions inside the system. Wet lay up for a short-term outage of the condensate/feedwater system: system filled with operating medium, avoiding air ingress into the system, injection of chemical agents if necessary. Dry lay-up or wet lay up for the steam generators depending on maintenance work or radiation protection needed. This article presents the EDF layup program and the improvements in layup practices. It provides information on the layup procedures, best practices and recommendations, operating experience, considerations for different durations of outages; the implementation of a preservation working group, the selection of the systems that will be included in the lay-up program. Finally, future developments will be presented, such as the development of a 'convective model' to determine air requirements (humidity, flow rate...) and the drying rate to dry a heat exchanger. (author)

  3. HVAC System Replacements for the Spanish Nuclear Fleet

    Energy Technology Data Exchange (ETDEWEB)

    Izquierdo, J.; Gensollen, T.; Pérez, C.

    2015-07-01

    The European Union and its Member States have established regulations to phase out ozone-depleting chlorofluorocarbons (CFCs). The chiller systems installed at the Spanish nuclear fleet contained zone depleting refrigerants (such as R-11, R-12, and R-22), which are being phased out of service. Due to the different material and thermodynamic properties of the replacement refrigerant (e.g. R-134A), a complete chiller system replacement is needed to comply with the EU regulations for CFCs. Delivering state of the art HVAC and Chiller systems that comply with the Nuclear Plant design basis, licensing basis, system and component specifications as well as European Union (EU) and Spanish codes and standards can be challenging for products purchased from US based manufacturers. Procurement specifications and Request for Quotes (RFQs) issued today for the procurement of original Plant components and systems will contain references to numerous codes and standards that were not in effect at the time the original components were specified and procured. The reference to EU and Spanish codes and standards that are unfamiliar to the HVAC suppliers can lead to uncertainty and concern related to specification compliance. The unnecessary burden of ambiguous codes and standards complicates the proposal process and introduces pricing uncertainty and contract risk. A review of the EU and Spanish national codes and standards that are often referenced in HVAC system related RFQs need to be performed to determine what codes and standards are applicable to HVAC systems designed, manufactured and tested in the US for export to Spain for installation in Spanish NPPs. Lessons learned and best practices should be applied to help both the Supplier (HVAC OEM) and the Purchaser Plant Operator) to optimize the procurement process and improve the quality of offerings to comply with applicable codes and standards. (Author)

  4. Averaged emission factors for the Hungarian car fleet

    Energy Technology Data Exchange (ETDEWEB)

    Haszpra, L. [Inst. for Atmospheric Physics, Budapest (Hungary); Szilagyi, I. [Central Research Inst. for Chemistry, Budapest (Hungary)

    1995-12-31

    The vehicular emission of non-methane hydrocarbon (NMHC) is one of the largest anthropogenic sources of NMHC in Hungary and in most of the industrialized countries. Non-methane hydrocarbon plays key role in the formation of photo-chemical air pollution, usually characterized by the ozone concentration, which seriously endangers the environment and human health. The ozone forming potential of the different NMHCs differs from each other significantly, while the NMHC composition of the car exhaust is influenced by the fuel and engine type, technical condition of the vehicle, vehicle speed and several other factors. In Hungary the majority of the cars are still of Eastern European origin. They represent the technological standard of the 70`s, although there are changes recently. Due to the long-term economical decline in Hungary the average age of the cars was about 9 years in 1990 and reached 10 years by 1993. The condition of the majority of the cars is poor. In addition, almost one third (31.2 %) of the cars are equipped with two-stroke engines which emit less NO{sub x} but much more hydrocarbon. The number of cars equipped with catalytic converter was negligible in 1990 and is slowly increasing only recently. As a consequence of these facts the traffic emission in Hungary may differ from that measured in or estimated for the Western European countries and the differences should be taken into account in the air pollution models. For the estimation of the average emission of the Hungarian car fleet a one-day roadway tunnel experiment was performed in the downtown of Budapest in summer, 1991. (orig.)

  5. Zika Virus Disrupts Phospho-TBK1 Localization and Mitosis in Human Neuroepithelial Stem Cells and Radial Glia

    Directory of Open Access Journals (Sweden)

    Marco Onorati

    2016-09-01

    Full Text Available The mechanisms underlying Zika virus (ZIKV-related microcephaly and other neurodevelopment defects remain poorly understood. Here, we describe the derivation and characterization, including single-cell RNA-seq, of neocortical and spinal cord neuroepithelial stem (NES cells to model early human neurodevelopment and ZIKV-related neuropathogenesis. By analyzing human NES cells, organotypic fetal brain slices, and a ZIKV-infected micrencephalic brain, we show that ZIKV infects both neocortical and spinal NES cells as well as their fetal homolog, radial glial cells (RGCs, causing disrupted mitoses, supernumerary centrosomes, structural disorganization, and cell death. ZIKV infection of NES cells and RGCs causes centrosomal depletion and mitochondrial sequestration of phospho-TBK1 during mitosis. We also found that nucleoside analogs inhibit ZIKV replication in NES cells, protecting them from ZIKV-induced pTBK1 relocalization and cell death. We established a model system of human neural stem cells to reveal cellular and molecular mechanisms underlying neurodevelopmental defects associated with ZIKV infection and its potential treatment.

  6. pH-tuneable binding of 2′-phospho-ADP-ribose to ketopantoate reductase: a structural and calorimetric study

    International Nuclear Information System (INIS)

    Ciulli, Alessio; Lobley, Carina M. C.; Tuck, Kellie L.; Smith, Alison G.; Blundell, Tom L.; Abell, Chris

    2007-01-01

    A combined crystallographic, calorimetric and mutagenic study has been used to show how changes in pH give rise to two distinct binding modes of 2′-phospho-ADP-ribose to ketopantoate reductase. The crystal structure of Escherichia coli ketopantoate reductase in complex with 2′-monophosphoadenosine 5′-diphosphoribose, a fragment of NADP + that lacks the nicotinamide ring, is reported. The ligand is bound at the enzyme active site in the opposite orientation to that observed for NADP + , with the adenine ring occupying the lipophilic nicotinamide pocket. Isothermal titration calorimetry with R31A and N98A mutants of the enzyme is used to show that the unusual ‘reversed binding mode’ observed in the crystal is triggered by changes in the protonation of binding groups at low pH. This research has important implications for fragment-based approaches to drug design, namely that the crystallization conditions and the chemical modification of ligands can have unexpected effects on the binding modes

  7. Recruitment of TREX to the transcription machinery by its direct binding to the phospho-CTD of RNA polymerase II.

    Directory of Open Access Journals (Sweden)

    Dominik M Meinel

    2013-11-01

    Full Text Available Messenger RNA (mRNA synthesis and export are tightly linked, but the molecular mechanisms of this coupling are largely unknown. In Saccharomyces cerevisiae, the conserved TREX complex couples transcription to mRNA export and mediates mRNP formation. Here, we show that TREX is recruited to the transcription machinery by direct interaction of its subcomplex THO with the serine 2-serine 5 (S2/S5 diphosphorylated CTD of RNA polymerase II. S2 and/or tyrosine 1 (Y1 phosphorylation of the CTD is required for TREX occupancy in vivo, establishing a second interaction platform necessary for TREX recruitment in addition to RNA. Genome-wide analyses show that the occupancy of THO and the TREX components Sub2 and Yra1 increases from the 5' to the 3' end of the gene in accordance with the CTD S2 phosphorylation pattern. Importantly, in a mutant strain, in which TREX is recruited to genes but does not increase towards the 3' end, the expression of long transcripts is specifically impaired. Thus, we show for the first time that a 5'-3' increase of a protein complex is essential for correct expression of the genome. In summary, we provide insight into how the phospho-code of the CTD directs mRNP formation and export through TREX recruitment.

  8. pH-tuneable binding of 2′-phospho-ADP-ribose to ketopantoate reductase: a structural and calorimetric study

    Energy Technology Data Exchange (ETDEWEB)

    Ciulli, Alessio [University Chemical Laboratory, Lensfield Road, Cambridge CB2 1EW (United Kingdom); Lobley, Carina M. C. [Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA (United Kingdom); Tuck, Kellie L. [University Chemical Laboratory, Lensfield Road, Cambridge CB2 1EW (United Kingdom); Smith, Alison G. [Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA (United Kingdom); Blundell, Tom L. [Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA (United Kingdom); Abell, Chris, E-mail: ca26@cam.ac.uk [University Chemical Laboratory, Lensfield Road, Cambridge CB2 1EW (United Kingdom)

    2007-02-01

    A combined crystallographic, calorimetric and mutagenic study has been used to show how changes in pH give rise to two distinct binding modes of 2′-phospho-ADP-ribose to ketopantoate reductase. The crystal structure of Escherichia coli ketopantoate reductase in complex with 2′-monophosphoadenosine 5′-diphosphoribose, a fragment of NADP{sup +} that lacks the nicotinamide ring, is reported. The ligand is bound at the enzyme active site in the opposite orientation to that observed for NADP{sup +}, with the adenine ring occupying the lipophilic nicotinamide pocket. Isothermal titration calorimetry with R31A and N98A mutants of the enzyme is used to show that the unusual ‘reversed binding mode’ observed in the crystal is triggered by changes in the protonation of binding groups at low pH. This research has important implications for fragment-based approaches to drug design, namely that the crystallization conditions and the chemical modification of ligands can have unexpected effects on the binding modes.

  9. Recruitment of TREX to the transcription machinery by its direct binding to the phospho-CTD of RNA polymerase II.

    Science.gov (United States)

    Meinel, Dominik M; Burkert-Kautzsch, Cornelia; Kieser, Anja; O'Duibhir, Eoghan; Siebert, Matthias; Mayer, Andreas; Cramer, Patrick; Söding, Johannes; Holstege, Frank C P; Sträßer, Katja

    2013-11-01

    Messenger RNA (mRNA) synthesis and export are tightly linked, but the molecular mechanisms of this coupling are largely unknown. In Saccharomyces cerevisiae, the conserved TREX complex couples transcription to mRNA export and mediates mRNP formation. Here, we show that TREX is recruited to the transcription machinery by direct interaction of its subcomplex THO with the serine 2-serine 5 (S2/S5) diphosphorylated CTD of RNA polymerase II. S2 and/or tyrosine 1 (Y1) phosphorylation of the CTD is required for TREX occupancy in vivo, establishing a second interaction platform necessary for TREX recruitment in addition to RNA. Genome-wide analyses show that the occupancy of THO and the TREX components Sub2 and Yra1 increases from the 5' to the 3' end of the gene in accordance with the CTD S2 phosphorylation pattern. Importantly, in a mutant strain, in which TREX is recruited to genes but does not increase towards the 3' end, the expression of long transcripts is specifically impaired. Thus, we show for the first time that a 5'-3' increase of a protein complex is essential for correct expression of the genome. In summary, we provide insight into how the phospho-code of the CTD directs mRNP formation and export through TREX recruitment.

  10. A heterodimer of human 3'-phospho-adenosine-5'-phosphosulphate (PAPS) synthases is a new sulphate activating complex

    International Nuclear Information System (INIS)

    Grum, Daniel; Boom, Johannes van den; Neumann, Daniel; Matena, Anja; Link, Nina M.; Mueller, Jonathan W.

    2010-01-01

    3'-Phospho-adenosine-5'-phosphosulphate (PAPS) synthases are fundamental to mammalian sulphate metabolism. These enzymes have recently been linked to a rising number of human diseases. Despite many studies, it is not yet understood how the mammalian PAPS synthases 1 and 2 interact with each other. We provide first evidence for heterodimerisation of these two enzymes by pull-down assays and Foerster resonance energy transfer (FRET) measurements. Kinetics of dimer dissociation/association indicates that these heterodimers form as soon as PAPSS1 and -S2 encounter each other in solution. Affinity of the homo- and heterodimers were found to be in the low nanomolar range using anisotropy measurements employing proteins labelled with the fluorescent dye IAEDANS that - in spite of its low quantum yield - is well suited for anisotropy due to its large Stokes shift. Within its kinase domain, the PAPS synthase heterodimer displays similar substrate inhibition by adenosine-5'-phosphosulphate (APS) as the homodimers. Due to divergent catalytic efficacies of PAPSS1 and -S2, the heterodimer might be a way of regulating PAPS synthase function within mammalian cells.

  11. Heuristic hybrid game approach for fleet condition-based maintenance planning

    International Nuclear Information System (INIS)

    Feng, Qiang; Bi, Xiong; Zhao, Xiujie; Chen, Yiran; Sun, Bo

    2017-01-01

    The condition-based maintenance (CBM) method is commonly used to select appropriate maintenance opportunities according to equipment status over a period of time. The CBM of aircraft fleets is a fleet maintenance planning problem. In this problem, mission requirements, resource constraints, and aircraft statuses are considered to find an optimal strategy set. Given that the maintenance strategies for each aircraft are finite, fleet CBM can be treated as a combinatorial optimization problem. In this study, the process of making a decision on the CBM of military fleets is analyzed. The fleet CBM problem is treated as a two-stage dynamic decision-making problem. Aircraft are divided into dispatch and standby sets; thus, the problem scale is significantly reduced. A heuristic hybrid game (HHG) approach comprising a competition game and a cooperative game is proposed on the basis of heuristic rule. In the dispatch set, a competition game approach is proposed to search for a local optimal strategy matrix. A cooperative game method for the two sets is also proposed to ensure global optimization. Finally, a case study regarding a fleet comprising 20 aircraft is conducted, with the results proving that the approach efficiently generates outcomes that meet the mission risk-oriented schedule requirement. - Highlights: • A new heuristic hybrid game method for fleet condition-based maintenance is proposed. • The problem is simplified by hierarchical solving based on dispatch and standby set. • The local optimal solution is got by competition game algorithm for dispatch set. • The global optimal solution is got by cooperative game algorithm between two sets.

  12. Addressing the minimum fleet problem in on-demand urban mobility.

    Science.gov (United States)

    Vazifeh, M M; Santi, P; Resta, G; Strogatz, S H; Ratti, C

    2018-05-01

    Information and communication technologies have opened the way to new solutions for urban mobility that provide better ways to match individuals with on-demand vehicles. However, a fundamental unsolved problem is how best to size and operate a fleet of vehicles, given a certain demand for personal mobility. Previous studies 1-5 either do not provide a scalable solution or require changes in human attitudes towards mobility. Here we provide a network-based solution to the following 'minimum fleet problem', given a collection of trips (specified by origin, destination and start time), of how to determine the minimum number of vehicles needed to serve all the trips without incurring any delay to the passengers. By introducing the notion of a 'vehicle-sharing network', we present an optimal computationally efficient solution to the problem, as well as a nearly optimal solution amenable to real-time implementation. We test both solutions on a dataset of 150 million taxi trips taken in the city of New York over one year 6 . The real-time implementation of the method with near-optimal service levels allows a 30 per cent reduction in fleet size compared to current taxi operation. Although constraints on driver availability and the existence of abnormal trip demands may lead to a relatively larger optimal value for the fleet size than that predicted here, the fleet size remains robust for a wide range of variations in historical trip demand. These predicted reductions in fleet size follow directly from a reorganization of taxi dispatching that could be implemented with a simple urban app; they do not assume ride sharing 7-9 , nor require changes to regulations, business models, or human attitudes towards mobility to become effective. Our results could become even more relevant in the years ahead as fleets of networked, self-driving cars become commonplace 10-14 .

  13. Remaining useful life estimation in heterogeneous fleets working under variable operating conditions

    International Nuclear Information System (INIS)

    Al-Dahidi, Sameer; Di Maio, Francesco; Baraldi, Piero; Zio, Enrico

    2016-01-01

    The availability of condition monitoring data for large fleets of similar equipment motivates the development of data-driven prognostic approaches that capitalize on the information contained in such data to estimate equipment Remaining Useful Life (RUL). A main difficulty is that the fleet of equipment typically experiences different operating conditions, which influence both the condition monitoring data and the degradation processes that physically determine the RUL. We propose an approach for RUL estimation from heterogeneous fleet data based on three phases: firstly, the degradation levels (states) of an homogeneous discrete-time finite-state semi-markov model are identified by resorting to an unsupervised ensemble clustering approach. Then, the parameters of the discrete Weibull distributions describing the transitions among the states and their uncertainties are inferred by resorting to the Maximum Likelihood Estimation (MLE) method and to the Fisher Information Matrix (FIM), respectively. Finally, the inferred degradation model is used to estimate the RUL of fleet equipment by direct Monte Carlo (MC) simulation. The proposed approach is applied to two case studies regarding heterogeneous fleets of aluminium electrolytic capacitors and turbofan engines. Results show the effectiveness of the proposed approach in predicting the RUL and its superiority compared to a fuzzy similarity-based approach of literature. - Highlights: • The prediction of the remaining useful life for heterogeneous fleets is addressed. • A data-driven prognostics approach based on a Markov model is proposed. • The proposed approach is applied to two different heterogeneous fleets. • The results are compared with those obtained by a fuzzy similarity-based approach.

  14. State and Alternative Fuel Provider Fleets Alternative Compliance; U.S. Department of Energy (DOE), Energy Efficiency & Renewable Energy (EERE)

    Energy Technology Data Exchange (ETDEWEB)

    None

    2015-08-01

    The final rule of the Energy Policy Act of 2005 and its associated regulations enable covered state and alternative fuel provider fleets to obtain waivers from the alternative fuel vehicle (AFV)-acquisition requirements of Standard Compliance. Under Alternative Compliance, covered fleets instead meet a petroleum-use reduction requirement. This guidance document is designed to help fleets better understand the Alternative Compliance option and successfully complete the waiver application process.

  15. The Radioactive Legacy of the Russian Pacific Fleet Operations and its Potential Impact on Neighboring Countries. Final Report

    International Nuclear Information System (INIS)

    Compton, K. L.; Novikov, V.M.; Parker, F.L.; Sivintsev, Y.U.

    2003-01-01

    There have been extensive studies of the current and potential environmental impact of Russian Northern fleet activities. However, despite the fact that the total number of ships in both fleets are comparable, there have been very few studies published in the open literature of the impact of the Pacific fleet. This study of the Pacific fleet's impact on neighboring countries was undertaken to partially remedy this lack of analysis. This study is focused on an evaluation of the inventory of major sources of radioactive material associated with the decommissioning of nuclear submarines, and an evaluation of releases to the atmosphere and their long-range (>100km) transboundary transport

  16. Adrián Muñoz. La piel de tigre y la serpiente: la identidad de los nāth-yoguis a través de sus leyendas

    Directory of Open Access Journals (Sweden)

    Luis Óscar Gómez Rodríguez

    2013-05-01

    Full Text Available El doctor Adrián Muñoz nos ofrece una obra erudita que demuestra un conocimiento amplio y detallado de una literatura que puede parecer muy especializada, incluso muy limitante en su particularidad y su superabundancia de textos y variantes textuales. Se trata de la literatura legendaria de un linaje hagiográfico complejo y heterogéneo, el nātha-sampradāya. Este linaje se asocia principalmente con la tradición hinduista, aunque también se entrelaza con la tradición de los 84 siddhas de la corriente budista. Muñoz se concentra en las narrativas legendarias y en el papel que desempeñan estas en la creación de la identidad religiosa de los seguidores del nāth-panth.

  17. Cost, Energy, and Environmental Impact of Automated Electric Taxi Fleets in Manhattan.

    Science.gov (United States)

    Bauer, Gordon S; Greenblatt, Jeffery B; Gerke, Brian F

    2018-04-17

    Shared automated electric vehicles (SAEVs) hold great promise for improving transportation access in urban centers while drastically reducing transportation-related energy consumption and air pollution. Using taxi-trip data from New York City, we develop an agent-based model to predict the battery range and charging infrastructure requirements of a fleet of SAEVs operating on Manhattan Island. We also develop a model to estimate the cost and environmental impact of providing service and perform extensive sensitivity analysis to test the robustness of our predictions. We estimate that costs will be lowest with a battery range of 50-90 mi, with either 66 chargers per square mile, rated at 11 kW or 44 chargers per square mile, rated at 22 kW. We estimate that the cost of service provided by such an SAEV fleet will be $0.29-$0.61 per revenue mile, an order of magnitude lower than the cost of service of present-day Manhattan taxis and $0.05-$0.08/mi lower than that of an automated fleet composed of any currently available hybrid or internal combustion engine vehicle (ICEV). We estimate that such an SAEV fleet drawing power from the current NYC power grid would reduce GHG emissions by 73% and energy consumption by 58% compared to an automated fleet of ICEVs.

  18. Fleet Planning Decision-Making: Two-Stage Optimization with Slot Purchase

    Directory of Open Access Journals (Sweden)

    Lay Eng Teoh

    2016-01-01

    Full Text Available Essentially, strategic fleet planning is vital for airlines to yield a higher profit margin while providing a desired service frequency to meet stochastic demand. In contrast to most studies that did not consider slot purchase which would affect the service frequency determination of airlines, this paper proposes a novel approach to solve the fleet planning problem subject to various operational constraints. A two-stage fleet planning model is formulated in which the first stage selects the individual operating route that requires slot purchase for network expansions while the second stage, in the form of probabilistic dynamic programming model, determines the quantity and type of aircraft (with the corresponding service frequency to meet the demand profitably. By analyzing an illustrative case study (with 38 international routes, the results show that the incorporation of slot purchase in fleet planning is beneficial to airlines in achieving economic and social sustainability. The developed model is practically viable for airlines not only to provide a better service quality (via a higher service frequency to meet more demand but also to obtain a higher revenue and profit margin, by making an optimal slot purchase and fleet planning decision throughout the long-term planning horizon.

  19. An Intelligent Fleet Condition-Based Maintenance Decision Making Method Based on Multi-Agent

    Directory of Open Access Journals (Sweden)

    Bo Sun

    2012-01-01

    Full Text Available According to the demand for condition-based maintenance online decision making among a mission oriented fleet, an intelligent maintenance decision making method based on Multi-agent and heuristic rules is proposed. The process of condition-based maintenance within an aircraft fleet (each containing one or more Line Replaceable Modules based on multiple maintenance thresholds is analyzed. Then the process is abstracted into a Multi-Agent Model, a 2-layer model structure containing host negotiation and independent negotiation is established, and the heuristic rules applied to global and local maintenance decision making is proposed. Based on Contract Net Protocol and the heuristic rules, the maintenance decision making algorithm is put forward. Finally, a fleet consisting of 10 aircrafts on a 3-wave continuous mission is illustrated to verify this method. Simulation results indicate that this method can improve the availability of the fleet, meet mission demands, rationalize the utilization of support resources and provide support for online maintenance decision making among a mission oriented fleet.

  20. Territorial organization in Spain and in Colombia: “Report on Spain. Rethinking the State or destroying it”. Santiago Muñoz Machado

    Directory of Open Access Journals (Sweden)

    Catalina Ana Larach Del Castillo

    2013-12-01

    Full Text Available Two recently published books, one from Spain (Muñoz Machado, 2012 and the other from Colombia (Mendoza Martelo, 2013, evince concerns with respect to the territorial organization in both countries. What is the appeal of the territorial model? With common constitutional errors occurring in the territorial area, and a crisis in both function and coordination in various levels of the Government –as well as in the different regions and autonomous communities–, is legal reform preferable to constitutional change? These are some of the issues raised and evaluated in current debates. In the work in question, the main problems in Spain are analyzed and the author proposes ways of solving or diminishing these problems, such as a return to centralism, the partial conservation of autonomous communities, the replacement of the current model with a federal State, as well as the possibilities of self-determination and separatism. The proposed study will serve to revise certain aspects of Colombia’s territorial system, in order to determine whether or not reformation is necessary.

  1. Phospho-carboxyl-terminal domain binding and the role of a prolyl isomerase in pre-mRNA 3'-End formation.

    Science.gov (United States)

    Morris, D P; Phatnani, H P; Greenleaf, A L

    1999-10-29

    A phospho-carboxyl-terminal domain (CTD) affinity column created with yeast CTD kinase I and the CTD of RNA polymerase II was used to identify Ess1/Pin1 as a phospho-CTD-binding protein. Ess1/Pin1 is a peptidyl prolyl isomerase involved in both mitotic regulation and pre-mRNA 3'-end formation. Like native Ess1, a GSTEss1 fusion protein associates specifically with the phosphorylated but not with the unphosphorylated CTD. Further, hyperphosphorylated RNA polymerase II appears to be the dominant Ess1 binding protein in total yeast extracts. We demonstrate that phospho-CTD binding is mediated by the small WW domain of Ess1 rather than the isomerase domain. These findings suggest a mechanism in which the WW domain binds the phosphorylated CTD of elongating RNA polymerase II and the isomerase domain reconfigures the CTD though isomerization of proline residues perhaps by a processive mechanism. This process may be linked to a variety of pre-mRNA maturation events that use the phosphorylated CTD, including the coupled processes of pre-mRNA 3'-end formation and transcription termination.

  2. Y-box Binding Protein-1 Enhances Oncogenic Transforming Growth Factor β Signaling in Breast Cancer Cells via Triggering Phospho-Activation of Smad2.

    Science.gov (United States)

    Stope, Matthias B; Weiss, Martin; Koensgen, Dominique; Popp, Simone L; Joffroy, Christian; Mustea, Alexander; Buck, Miriam B; Knabbe, Cornelius

    2017-12-01

    Transforming growth factor β (TGFβ) plays a role in diverse oncogenic pathways including cell proliferation and cell motility and is regulated by the pleiotropic factor Y-box binding protein-1 (YB-1). In breast cancer, Sma/Mad related protein 2 (Smad2) represents the most common downstream transducer in TGFβ signaling. Here, YB-1's impact on Smad2 phospho-activation was characterized by incubation of the breast cancer cell line MCF-7 with or without TGFβ1 in the absence or presence of overexpressed YB-1 protein. The phospho-status of Smad2 was assessed via western blotting. Analysis of MCF-7 cells revealed no induction of total Smad2 neither in the presence of TGFβ1, nor during YB-1 overexpression. In contrast, incubation with TGFβ1 led to an increase of phosphorylated Smad2 forms which was significantly amplified by simultaneously overexpressed YB-1 (2.8±0.2-fold). Oncogenic YB-1 indirectly enhances TGFβ signaling cascades via Smad2 phospho-activation and may represent a promising factor for future diagnosis and therapy of breast cancer. Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

  3. Uranium in phosphate rocks and future nuclear power fleets

    International Nuclear Information System (INIS)

    Gabriel, S.; Baschwitz, A.; Mathonniere, G.

    2014-01-01

    phosphoric acid. Uranium recovery as a by-product of phosphate rocks could be competitive for the moment, but limited at the most to 10 kt U per year, i.e. less than 20% of current world demand. The only way to lift the constraint of capacity production is to produce uranium as a primary product of phosphates. Unfortunately, this solution is very unlikely due to its high unit cost. In line with these considerations, the correspondence between the estimated resources and the forecast energy scenarios is examined, first with the current type of light water reactors which burn uranium, and secondly with a mixed fleet with both light water reactors and fast reactors which use plutonium. (author)

  4. A multiscale forecasting method for power plant fleet management

    Science.gov (United States)

    Chen, Hongmei

    In recent years the electric power industry has been challenged by a high level of uncertainty and volatility brought on by deregulation and globalization. A power producer must minimize the life cycle cost while meeting stringent safety and regulatory requirements and fulfilling customer demand for high reliability. Therefore, to achieve true system excellence, a more sophisticated system-level decision-making process with a more accurate forecasting support system to manage diverse and often widely dispersed generation units as a single, easily scaled and deployed fleet system in order to fully utilize the critical assets of a power producer has been created as a response. The process takes into account the time horizon for each of the major decision actions taken in a power plant and develops methods for information sharing between them. These decisions are highly interrelated and no optimal operation can be achieved without sharing information in the overall process. The process includes a forecasting system to provide information for planning for uncertainty. A new forecasting method is proposed, which utilizes a synergy of several modeling techniques properly combined at different time-scales of the forecasting objects. It can not only take advantages of the abundant historical data but also take into account the impact of pertinent driving forces from the external business environment to achieve more accurate forecasting results. Then block bootstrap is utilized to measure the bias in the estimate of the expected life cycle cost which will actually be needed to drive the business for a power plant in the long run. Finally, scenario analysis is used to provide a composite picture of future developments for decision making or strategic planning. The decision-making process is applied to a typical power producer chosen to represent challenging customer demand during high-demand periods. The process enhances system excellence by providing more accurate market

  5. Anthranilate phosphoribosyltransferase: Binding determinants for 5'-phospho-alpha-d-ribosyl-1'-pyrophosphate (PRPP) and the implications for inhibitor design.

    Science.gov (United States)

    Evans, Genevieve L; Furkert, Daniel P; Abermil, Nacim; Kundu, Preeti; de Lange, Katrina M; Parker, Emily J; Brimble, Margaret A; Baker, Edward N; Lott, J Shaun

    2018-02-01

    Phosphoribosyltransferases (PRTs) bind 5'-phospho-α-d-ribosyl-1'-pyrophosphate (PRPP) and transfer its phosphoribosyl group (PRib) to specific nucleophiles. Anthranilate PRT (AnPRT) is a promiscuous PRT that can phosphoribosylate both anthranilate and alternative substrates, and is the only example of a type III PRT. Comparison of the PRPP binding mode in type I, II and III PRTs indicates that AnPRT does not bind PRPP, or nearby metals, in the same conformation as other PRTs. A structure with a stereoisomer of PRPP bound to AnPRT from Mycobacterium tuberculosis (Mtb) suggests a catalytic or post-catalytic state that links PRib movement to metal movement. Crystal structures of Mtb-AnPRT in complex with PRPP and with varying occupancies of the two metal binding sites, complemented by activity assay data, indicate that this type III PRT binds a single metal-coordinated species of PRPP, while an adjacent second metal site can be occupied due to a separate binding event. A series of compounds were synthesized that included a phosphonate group to probe PRPP binding site. Compounds containing a "bianthranilate"-like moiety are inhibitors with IC 50 values of 10-60μM, and K i values of 1.3-15μM. Structures of Mtb-AnPRT in complex with these compounds indicate that their phosphonate moieties are unable to mimic the binding modes of the PRib or pyrophosphate moieties of PRPP. The AnPRT structures presented herein indicated that PRPP binds a surface cleft and becomes enclosed due to re-positioning of two mobile loops. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. Localization of the 5-phospho-alpha-D-ribosyl-1-pyrophosphate binding site of human hypoxanthine-guanine phosphoribosyltransferase.

    Science.gov (United States)

    Keough, D T; Emmerson, B T; de Jersey, J

    1991-02-22

    Human erythrocyte hypoxanthine-guanine phosphoribosyltransferase (HPRT) is inactivated by iodoacetate in the absence, but not in the presence, of the substrate, 5-phospho-alpha-D-ribosyl-1-pyrophosphate (PRib-PP). Treatment of HPRT with [14C]iodoacetate followed by tryptic digestion, peptide separation and sequencing has shown that Cys-22 reacts with iodoacetate only in the absence of PRib-PP; this strongly suggests that Cys-22 is in or near the PRib-PP binding site. In contrast, Cys-105 reacts with [14C]iodoacetate both in the presence and absence of PRib-PP. Carboxymethylation of Cys-22 resulted in an increase in the Km for PRib-PP, but no change in Vmax. Storage of HPRT also resulted in an increase in the Km for PRib-PP and a decrease in its susceptibility to inactivation by iodoacetate. Dialysis of stored enzyme against 1 mM dithiothreitol resulted in a marked decrease in Km for PRib-PP. The stoichiometry of the reaction of [14C]iodoacetate with Cys-22 in HPRT leading to inactivation (approx. 1 residue modified per tetramer) showed that, in this preparation of HPRT purified from erythrocytes, only about 25% of the Cys-22 side chains were present as free and accessible thiols. Titration of thiol groups [with 5,5'-dithiobis(2-nitrobenzoic acid)] and the effect of dithiothreitol on Km for PRib-PP indicate that oxidation of thiol groups occurs on storage of HPRT, even in the presence of 1 mM beta-mercaptoethanol.

  7. Passive immunization with phospho-tau antibodies reduces tau pathology and functional deficits in two distinct mouse tauopathy models.

    Directory of Open Access Journals (Sweden)

    Sethu Sankaranarayanan

    Full Text Available In Alzheimer's disease (AD, an extensive accumulation of extracellular amyloid plaques and intraneuronal tau tangles, along with neuronal loss, is evident in distinct brain regions. Staging of tau pathology by postmortem analysis of AD subjects suggests a sequence of initiation and subsequent spread of neurofibrillary tau tangles along defined brain anatomical pathways. Further, the severity of cognitive deficits correlates with the degree and extent of tau pathology. In this study, we demonstrate that phospho-tau (p-tau antibodies, PHF6 and PHF13, can prevent the induction of tau pathology in primary neuron cultures. The impact of passive immunotherapy on the formation and spread of tau pathology, as well as functional deficits, was subsequently evaluated with these antibodies in two distinct transgenic mouse tauopathy models. The rTg4510 transgenic mouse is characterized by inducible over-expression of P301L mutant tau, and exhibits robust age-dependent brain tau pathology. Systemic treatment with PHF6 and PHF13 from 3 to 6 months of age led to a significant decline in brain and CSF p-tau levels. In a second model, injection of preformed tau fibrils (PFFs comprised of recombinant tau protein encompassing the microtubule-repeat domains into the cortex and hippocampus of young P301S mutant tau over-expressing mice (PS19 led to robust tau pathology on the ipsilateral side with evidence of spread to distant sites, including the contralateral hippocampus and bilateral entorhinal cortex 4 weeks post-injection. Systemic treatment with PHF13 led to a significant decline in the spread of tau pathology in this model. The reduction in tau species after p-tau antibody treatment was associated with an improvement in novel-object recognition memory test in both models. These studies provide evidence supporting the use of tau immunotherapy as a potential treatment option for AD and other tauopathies.

  8. Neighboring phosphoSer-Pro motifs in the undefined domain of IRAK1 impart bivalent advantage for Pin1 binding.

    Science.gov (United States)

    Rogals, Monique J; Greenwood, Alexander I; Kwon, Jeahoo; Lu, Kun Ping; Nicholson, Linda K

    2016-12-01

    The peptidyl prolyl isomerase Pin1 has two domains that are considered to be its binding (WW) and catalytic (PPIase) domains, both of which interact with phosphorylated Ser/Thr-Pro motifs. This shared specificity might influence substrate selection, as many known Pin1 substrates have multiple sequentially close phosphoSer/Thr-Pro motifs, including the protein interleukin-1 receptor-associated kinase-1 (IRAK1). The IRAK1 undefined domain (UD) contains two sets of such neighboring motifs (Ser131/Ser144 and Ser163/Ser173), suggesting possible bivalent interactions with Pin1. Using a series of NMR titrations with 15N-labeled full-length Pin1 (Pin1-FL), PPIase, or WW domain and phosphopeptides representing the Ser131/Ser144 and Ser163/Ser173 regions of IRAK1-UD, bivalent interactions were investigated. Binding studies using singly phosphorylated peptides showed that individual motifs displayed weak affinities (> 100 μm) for Pin1-FL and each isolated domain. Analysis of dually phosphorylated peptides binding to Pin1-FL showed that inclusion of bivalent states was necessary to fit the data. The resulting complex model and fitted parameters were applied to predict the impact of bivalent states at low micromolar concentrations, demonstrating significant affinity enhancement for both dually phosphorylated peptides (3.5 and 24 μm for peptides based on the Ser131/Ser144 and Ser163/Ser173 regions, respectively). The complementary technique biolayer interferometry confirmed the predicted affinity enhancement for a representative set of singly and dually phosphorylated Ser131/Ser144 peptides at low micromolar concentrations, validating model predictions. These studies provide novel insights regarding the complexity of interactions between Pin1 and activated IRAK1, and more broadly suggest that phosphorylation of neighboring Ser/Thr-Pro motifs in proteins might provide competitive advantage at cellular concentrations for engaging with Pin1. © 2016 Federation of European

  9. Simultaneous analysis of proteome, phospho- and glycoproteome of rat kidney tissue with electrostatic repulsion hydrophilic interaction chromatography.

    Directory of Open Access Journals (Sweden)

    Piliang Hao

    Full Text Available Protein post-translational modifications (PTMs are regulated separately from protein expression levels. Thus, simultaneous characterization of the proteome and its PTMs is pivotal to an understanding of protein regulation, function and activity. However, concurrent analysis of the proteome and its PTMs by mass spectrometry is a challenging task because the peptides bearing PTMs are present in sub-stoichiometric amounts and their ionization is often suppressed by unmodified peptides of high abundance. We describe here a method for concurrent analysis of phosphopeptides, glycopeptides and unmodified peptides in a tryptic digest of rat kidney tissue with a sequence of ERLIC and RP-LC-MS/MS in a single experimental run, thereby avoiding inter-experimental variation. Optimization of loading solvents and elution gradients permitted ERLIC to be performed with totally volatile solvents. Two SCX and four ERLIC gradients were compared in details, and one ERLIC gradient was found to perform the best, which identified 2929 proteins, 583 phosphorylation sites in 338 phosphoproteins and 722 N-glycosylation sites in 387 glycoproteins from rat kidney tissue. Two hundred low-abundance proteins with important functions were identified only from the glyco- or phospho-subproteomes, reflecting the importance of the enrichment and separation of modified peptides by ERLIC. In addition, this strategy enables identification of unmodified and corresponding modified peptides (partial phosphorylation and N-glycosylation from the same protein. Interestingly, partially modified proteins tend to occur on proteins involved in transport. Moreover, some membrane or extracellular proteins, such as versican core protein and fibronectin, were found to have both phosphorylation and N-glycosylation, which may permit an assessment of the potential for cross talk between these two vital PTMs and their roles in regulation.

  10. Phospho-Ibuprofen (MDC-917) Is a Novel Agent against Colon Cancer: Efficacy, Metabolism, and Pharmacokinetics in Mouse Models

    Science.gov (United States)

    Xie, Gang; Sun, Yu; Nie, Ting; Mackenzie, Gerardo G.; Huang, Liqun; Kopelovich, Levy; Komninou, Despina

    2011-01-01

    We have developed a novel chemical modification of conventional nonsteroidal anti-inflammatory drugs to reduce their toxicity and enhance their efficacy. Phospho-ibuprofen [(PI) 2-(4-isobutyl-phenyl)-propionic acid-4-(diethoxy-phosphoryloxy)-butyl ester (MDC-917)], a novel derivative of ibuprofen, strongly inhibited the growth of human colon cancer cells in vitro and SW480 human colon cancer xenografts in nude mice. PI was metabolized minimally by cultured cells, but extensively by liver microsomes and mice, undergoing regioselective oxidation to produce 1-OH-PI and carboxyl-PI, which can be hydrolyzed to 1-OH-ibuprofen and carboxyl-ibuprofen, respectively. PI also can be hydrolyzed to release ibuprofen, which can generate 2-OH-ibuprofen, carboxyl-ibuprofen, and ibuprofen glucuronide. After a single oral administration (400 mg/kg) of PI, ibuprofen and ibuprofen glucuronide are the main plasma metabolites of PI; they have, respectively, Cmax of 530 and 215 μM, Tmax of 1 and 2 h, elimination t1/2 of 7.7 and 5.3 h, and area under the concentration-time curve (0–24 h) of 1816 and 832 μM × h. Intact PI was detected in several tissues but not in plasma; at a higher PI dose (1200 mg/kg), PI plasma levels were 12.4 μM. PI generated the same metabolites in mouse plasma as conventional ibuprofen, but with much lower levels, perhaps accounting for the enhanced safety of PI. The antitumor effect of PI was significantly associated with plasma ibuprofen levels (p = 0.016) but not with xenograft ibuprofen levels (p = 0.08), suggesting a complex anticancer effect. These results provide a pharmacological basis to explain, at least in part, the anticancer efficacy and safety of this promising compound and indicate that PI merits further evaluation as an anticancer agent. PMID:21422165

  11. New logistical issues in using electric vehicle fleets with battery exchange infrastructure

    DEFF Research Database (Denmark)

    Mirchandani, Pitu; Adler, Jonathan; Madsen, Oli B.G.

    2014-01-01

    There is much reason to believe that fleets of service vehicles of many organizations will transform their vehicles that utilize alternative fuels that are more sustainable. The electric vehicle (EV) is a good candidate for this transformation, especially which "refuels" by exchanging its spent...... batteries with charged ones. This paper discusses some new logistical issues that must be addressed by such EV fleets, principally the issues related to the limited driving range of each EV's set of charged batteries and the possible detouring for battery exchanges. In particular, the paper addresses (1......) the routing and scheduling of the fleet, (2) the locations of battery-exchange stations, and (3) the sizing of each facility. An overview of the literature on the topic is provided and some initial results are presented. (C) 2013 The Authors. Published by Elsevier Ltd....

  12. Increased sharing of renewable energies in the electricity production system: what impact on the reactor fleet?

    International Nuclear Information System (INIS)

    Cany, C.; Devezeaux de Lavergne, J.G.; Mansilla, C.; Mathonniere, G.

    2017-01-01

    This article presents the flexibility of an individual reactor and of the complete fleet of reactors as a means to cope with the variability of renewable energies like solar or wind energies. Flexibility means the ability for load following and this ability is limited by both safety rules and limits on the release of radionuclides in the environment. The flexibility of the fleet depends on individual reactor flexibility but also on organisational and economic constraints. The participation of a reactor to load following depends on: its availability (not in maintenance or testing phase), its position in the cycle, the positioning of its scheduled shutdowns and the minimization of the volume of effluents. The study presents the future need of flexibility for the reactor fleet as the shares of wind and solar energies increase in the French energy mix. (A.C.)

  13. Fleet Evaluation and Factory Installation of Aerodynamic Heavy Duty Truck Trailers

    Energy Technology Data Exchange (ETDEWEB)

    Beck, Jason; Salari, Kambiz; Ortega, Jason; Brown, Andrea

    2013-09-30

    The purpose of DE-EE0001552 was to develop and deploy a combination of trailer aerodynamic devices and low rolling resistance tires that reduce fuel consumption of a class 8 heavy duty tractor-trailer combination vehicle by 15%. There were 3 phases of the project: Phase 1 – Perform SAE Typed 2 track tests with multiple device combinations. Phase 2 – Conduct a fleet evaluation with selected device combination. Phase 3 – Develop the devices required to manufacture the aerodynamic trailer. All 3 phases have been completed. There is an abundance of available trailer devices on the market, and fleets and owner operators have awareness of them and are purchasing them. The products developed in conjunction with this project are at least in their second round of refinement. The fleet test undertaken showed an improvement of 5.5 – 7.8% fuel economy with the devices (This does not include tire contribution).

  14. A multi-species multi-fleet bioeconomic simulation model for the English Channel artisanal fisheries

    DEFF Research Database (Denmark)

    Ulrich, Clara; Le Gallic, B.; Dunn, M.R.

    2002-01-01

    Considering the large number of technical interactions between various fishing activities, the English Channel (ICES divisions VIId and VIIe) fisheries may be regarded as one large and diverse multi-country, multi-gear and multi-species artisanal fishery, although rarely studied as such. A whole...... of the model is to study the long-term consequences of various management alternatives on the economic situation of the English and French fleets fishing in the area and on exploited resources. The model describes this feature through the links between three entities on the one hand (stocks, fleets...... and "metiers", i.e. gear x target species x fishing area), and three modules on the other hand (activity, biological production and economics). The model is described and some simulation results are presented. An example simulating a decrease of one fleet segment effort illustrates these technical interactions...

  15. Assessment of Fleet Inventory for Naval Air Station Whidbey Island. Task 1

    Energy Technology Data Exchange (ETDEWEB)

    Schey, Stephen [Idaho National Lab. (INL), Idaho Falls, ID (United States); Francfort, Jim [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-06-01

    Task 1includes a survey of the inventory of non-tactical fleet vehicles at Naval Air Station Whidbey Island (NASWI) to characterize the fleet. This information and characterization are used to select vehicles for monitoring that takes place during Task 2. This monitoring involves data logging of vehicle operation in order to identify the vehicle’s mission and travel requirements. Individual observations of these selected vehicles provide the basis for recommendations related to PEV adoption. It also identifies whether a battery electric vehicle or plug-in hybrid electric vehicle (collectively referred to as PEVs) can fulfill the mission requirements and provide observations related to placement of PEV charging infrastructure. This report provides the results of the assessments and observations of the current non-tactical fleet, fulfilling the Task 1 requirements.

  16. Fleet Numerical Meteorology and Oceanography Center support for GODAE

    Science.gov (United States)

    Dimitriou, D.; Sharfstein, P.; Ignaszewski, M.; Clancy, M.

    2003-04-01

    The U.S. Navy's Fleet Numerical Meteorology and Oceanography Center (FNMOC; see http://www.fnmoc.navy.mil/), located in Monterey, CA, is the lead activity within the U.S. Department of Defense (DoD) for numerical weather prediction and coupled air-sea modeling. FNMOC fulfills this role through means of a suite of sophisticated global and regional meteorological and oceanographic (METOC) models, extending from the top of the atmosphere to the bottom of the ocean, which is supported by one of the world's most complete real-time METOC databases. Fleet Numerical operates around-the-clock, 365 days per year and distributes METOC products to military and civilian users around the world, both ashore and afloat, through a variety of means, including a rapidly growing and innovative use of Web technology. FNMOC's customers include all branches of the Department of Defense (DoD), other government organizations such as the National Weather Service, private companies such as the Weather Channel, a number of colleges and universities, and the general public. FNMOC acquires and processes over 6 million METOC observations per day—creating one of the world's most comprehensive real-time databases of meteorological and oceanographic observations for assimilation into its models. FNMOC employs three primary models, the Navy Operational Global Atmospheric Prediction System (NOGAPS), the Coupled Ocean/Atmosphere Mesoscale Prediction System (COAMPS), and the WaveWatch III model (WW3), along with a number of specialized models and related applications. NOGAPS is a global weather model, driving nearly all other FNMOC models and applications in some fashion. COAMPS is a high-resolution regional model that has proved to be particularly valuable for forecasting weather and ocean conditions in highly complex coastal areas. WW3 is a state-of-the-art ocean wave model that is employed both globally and regionally in support of a wide variety of naval operations. Specialized models support and

  17. Greenhouse gas implications of fleet electrification based on big data-informed individual travel patterns.

    Science.gov (United States)

    Cai, Hua; Xu, Ming

    2013-08-20

    Environmental implications of fleet electrification highly depend on the adoption and utilization of electric vehicles at the individual level. Past research has been constrained by using aggregated data to assume all vehicles with the same travel pattern as the aggregated average. This neglects the inherent heterogeneity of individual travel behaviors and may lead to unrealistic estimation of environmental impacts of fleet electrification. Using "big data" mining techniques, this research examines real-time vehicle trajectory data for 10,375 taxis in Beijing in one week to characterize the travel patterns of individual taxis. We then evaluate the impact of adopting plug-in hybrid electric vehicles (PHEV) in the taxi fleet on life cycle greenhouse gas emissions based on the characterized individual travel patterns. The results indicate that 1) the largest gasoline displacement (1.1 million gallons per year) can be achieved by adopting PHEVs with modest electric range (approximately 80 miles) with current battery cost, limited public charging infrastructure, and no government subsidy; 2) reducing battery cost has the largest impact on increasing the electrification rate of vehicle mileage traveled (VMT), thus increasing gasoline displacement, followed by diversified charging opportunities; 3) government subsidies can be more effective to increase the VMT electrification rate and gasoline displacement if targeted to PHEVs with modest electric ranges (80 to 120 miles); and 4) while taxi fleet electrification can increase greenhouse gas emissions by up to 115 kiloton CO2-eq per year with the current grid in Beijing, emission reduction of up to 36.5 kiloton CO2-eq per year can be achieved if the fuel cycle emission factor of electricity can be reduced to 168.7 g/km. Although the results are based on a specific public fleet, this study demonstrates the benefit of using large-scale individual-based trajectory data (big data) to better understand environmental implications

  18. Application of GPS data for benefits of air quality assessment and fleet management

    Science.gov (United States)

    Hao, Song; Fat Lam, Yun; Cheong Ying, Chi; Chan, Ka Lok

    2017-04-01

    In the modern digitizedsociety, traffic data can be easily collected for use in roadway development, urban planning and vehicle emission. These data are then further parameterized to support traffic simulation and roadside emission calculations. With the commercialization of AGPS/GPS technology, GPS data are widely utilized to study habit and travelling behaviors. GPS on franchised buses can provide not only positioning information for fleet management but also raw data to analyze traffic situations. In HK, franchised buses account for 6% of RSP and 20% of NOx emissions among the whole vehicle fleet. Being the most heavily means of public transport, the setting up of bus travelling trajectories and service frequency always raise concern from citizens. On this basis, there is an increasing interest and as well as to design and realize an effective cost benefit fleet management strategy. In this study, data collection analysis is carried out on all bus routes (i.e. 112) in Shatin district, one of the 18 districts in Hong Kong. The GPS/AGPS data through Esri ArcGIS investigate the potential benefit of GPS data in different emission scenarios (such as engine type over whole bus fleet). Building on the emission factors from EMFC-HK model, we accounted for factors like travelling distance, idling time, occupancy rate, service frequency, tire and break emissions. Through the simple emission developed model we demonstrate how GPS are data are utilized to assess bus fleet emissions. Further amelioration on the results involve tuning the model with field measurement so as to assess district level emission change after fleet optimization.

  19. INTEGRAL ASSESSMENT OF EFFICIENCY OF A FLEET OF AGRICULTURAL MACHINERY AND TRACTORS

    Directory of Open Access Journals (Sweden)

    V. M. Korotchenya

    2015-01-01

    Full Text Available An indicator for an integral assessment of efficiency of a fleet of agricultural machinery and tractors is proposed. Its calculating consists in multiplying partial efficiencies together: technical and price efficiency of agricultural production, and eco-efficiency of a fleet of agricultural machinery and tractors. Using an axiomatic method, the concept of efficiency of fleet was studied within broader category of productivity and in wider scales - within agriculture. The most general and obvious statements according to which efficiency of machine and tractor fleet is considered as indirectly settlement size counted proceeding from efficiency of agricultural production, were accepted as axioms. In general, efficiency is a ratio between productivity of an object in question and that of the theoretical ideal or an object with maximum productivity efficiency of which is accepted equal to unity or 100%. Technical efficiency of agricultural production characterizes the ability of an agricultural sector to produce the technically maximum output of agricultural produce obtained from the available resources (land, labor, machines, etc.. Price efficiency evaluates the ability of an agricultural sector to produce output of agricultural produce with regard to employing the optimal mix of resources given their prices. Because the theoretical ideal does not exist, efficiency of an agricultural sector, e.g. in Russia and of its fleet of agricultural machinery and tractors, is measured against agriculture of another country with maximum productivity efficiency of which is accepted as equal to unity or 100 percent. The calculation can be made on the basis of the Data Envelopment Analysis method. It is possible to calculate price and economic efficiency of Russian agriculture (fleet of agricultural machinery and tractors due to use its domestic prices.

  20. CEMENT TRANSPORTATION LIMITED-FLEET MODELING AND ASSIGNING TO RATED DEMANDS

    Directory of Open Access Journals (Sweden)

    Narjes MASHHADI BANDANI

    2017-04-01

    Full Text Available Transportation is an inseparable part of the supply chain, with a key role in product distribution. This role is highlighted when ratio of “the cost of transportation” to “the value of goods” such as cement is significant. Iran has recently become one of the main centers of cement production in the world. However, transportation is the most important challenge in cement distribution because of weak structure of the transportation fleet and its independent action. Independence of and lack of commitment on the part of transportation fleets to cement companies as well as lack of timely delivery due to shortage of transportation in some routes and seasons lead to customers` dissatisfaction and even market loss or lack of market development. One of the significant differences between the transportation system in Iran and that in developed countries is lack of complete productivity of the transportation fleet. It means that trucks are driver-based in Iran. This paper introduces a model considering some issues such as driver-based trucks, size of the transportation fleet based on the number of active trucks, and demand priorities in the cement company. Taking the relation between the number of active trucks and the cement company into account, this model assigns weekly demands to the transportation fleet. It also tries to minimize the delay to respond to demands and increases the efficiency of the transportation fleet. Finally, this current condition-based model is compared with two other models including “no constraints on different routes of trucks” as well as single-route model for trucks.

  1. Correlating Dynamometer Testing to In-Use Fleet Results of Plug-In Hybrid Electric Vehicles

    Energy Technology Data Exchange (ETDEWEB)

    John G. Smart; Sera White; Michael Duoba

    2009-05-01

    Standard dynamometer test procedures are currently being developed to determine fuel and electrical energy consumption of plug-in hybrid vehicles (PHEV). To define a repeatable test procedure, assumptions were made about how PHEVs will be driven and charged. This study evaluates these assumptions by comparing results of PHEV dynamometer testing following proposed procedures to actual performance of PHEVs operating in the US Department of Energy’s (DOE) North American PHEV Demonstration fleet. Results show PHEVs in the fleet exhibit a wide range of energy consumption, which is not demonstrated in dynamometer testing. Sources of variation in performance are identified and examined.

  2. Fleet view of electrified transportation reveals smaller potential to reduce GHG emissions

    International Nuclear Information System (INIS)

    Meinrenken, Christoph J.; Lackner, Klaus S.

    2015-01-01

    Highlights: • Novel framework compares GHG of plugins vs. hybrids for any vehicle type/performance. • Fleet GHG can be compared without forecasting market penetrations of vehicle sizes. • GHG/km for pure electrics must account for limited range using novel, modified Utility Factor. • Applied to the US, this points to smaller GHG reduction at fleet level than traditional fleet analyses. - Abstract: Plugin and hybrid vehicles have been shown to offer possible reductions in greenhouse gas (GHG) emissions, depending on grid-carbon-intensity, range and thus life-cycle battery emissions and vehicle weight, and on trip patterns. We present a framework that enables GHG comparisons (well-to-wheel plus storage manufacturing) for three drivetrains (pure-electric, gasoline-hybrid, and plugin-hybrid), both for individual vehicles and for fleets. The framework captures effects of grid- versus vehicle-based electricity generation, grid transmission and charging losses, and manufacturing and carrying batteries. In contrast to previous work, GHG comparisons can be obtained for heterogeneous fleets of varying vehicle sizes (cars, vans, buses, trucks) and performances, without requiring forecasting of such vehicle specs and their respective market penetrations. Further, we show how a novel adaptation of the Utility Factor concept from plug-in-hybrids to mixed fleets of battery-only and gasoline-hybrids is crucial to quantifying battery-only-vehicles’ impact on fleet-wide GHG. To account for regional variations and possible future technology improvements, we show scenarios over a wide spectrum of grid-carbon-intensities (50–1200 g CO 2 e/kW h at wall), vehicle range (∼5–500 km), battery energy densities, and battery life-cycle GHG. Model uncertainties are quantified via sensitivity tests. Applying the framework to trip patterns of US passenger transportation, we find that owing to the interplay of GHG/km, battery size, all-electric range, and trip patterns, GHG

  3. MPC and A enhancements for the Murmansk shipping company icebreaker fleet

    International Nuclear Information System (INIS)

    Bartoch, O.; Bondarev, N.; Caskey, D.; Forehand, M.; Lambert, D.; Maltsev, V.; O'Brien, M.; Gardner, B.; Tittemore, G.

    1999-01-01

    The United States and the Russian Federation entered into a cooperative agreement in 1994 that resulted in a nuclear weapons non-proliferation program within the United States (US) Department of Energy (DOE) currently known as the Russia/Newly Independent States (NIS) Nuclear Material Security Task Force. In 1996, a project was initiated with the Murmansk Shipping Company to enhance material protection, control, and accounting of highly enriched nuclear fuel assemblies used for the Icebreaker Fleet. The commissioning ceremony for this project is scheduled for August 1999. This paper describes the physical protection, material control, and accounting measures implemented for the Icebreaker Fleet

  4. Lessons from cross-fleet/cross-airline observations - Evaluating the impact of CRM/LOFT training

    Science.gov (United States)

    Butler, Roy E.

    1991-01-01

    A review is presented of the crew resource management/line oriented flight training (CRM/LOFT) program to help determine the level of standardization across fleets and airlines in the critical area of evaluating crew behavior and performance. One of the goals of the project is to verify that check airmen and LOFT instructors within organizations are evaluating CRM issues consistently and that differences observed between fleets are not a function of idiosyncracies on the part of observers. Attention is given to the research tools for crew evaluation.

  5. What Fleets Need to Know About Alternative Fuel Vehicle Conversions, Retrofits, and Repowers

    Energy Technology Data Exchange (ETDEWEB)

    Kelly, K.; Gonzales, J.

    2017-10-02

    Many fleet managers have opted to incorporate alternative fuels and advanced vehicles into their lineup. Original equipment manufacturers (OEMs) offer a variety of choices, and there are additional options offered by aftermarket companies. There are also a myriad of ways that existing vehicles can be modified to utilize alternative fuels and other advanced technologies. Vehicle conversions and retrofit packages, along with engine repower options, can offer an ideal way to lower vehicle operating costs. This can result in long term return on investment, in addition to helping fleet managers achieve emissions and environmental goals. This report summarizes the various factors to consider when pursuing a conversion, retrofit, or repower option.

  6. What Fleets Need to Know About Alternative Fuel Vehicle Conversions, Retrofits, and Repowers

    Energy Technology Data Exchange (ETDEWEB)

    Kelly, Kay L. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Gonzales, John [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2017-10-17

    Many fleet managers have opted to incorporate alternative fuels and advanced vehicles into their lineup. Original equipment manufacturers (OEMs) offer a variety of choices, and there are additional options offered by aftermarket companies. There are also a myriad of ways that existing vehicles can be modified to utilize alternative fuels and other advanced technologies. Vehicle conversions and retrofit packages, along with engine repower options, can offer an ideal way to lower vehicle operating costs. This can result in long term return on investment, in addition to helping fleet managers achieve emissions and environmental goals. This report summarizes the various factors to consider when pursuing a conversion, retrofit, or repower option.

  7. AVTA Federal Fleet PEV Readiness Data Logging and Characterization Study for NASA White Sands Test Facility

    Energy Technology Data Exchange (ETDEWEB)

    Schey, Stephen [Idaho National Lab. (INL), Idaho Falls, ID (United States); Francfort, Jim [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2014-10-01

    This report focuses on the NASA White Sands Test Facility (WSTF) fleet to identify daily operational characteristics of select vehicles and report findings on vehicle and mission characterizations to support the successful introduction of plug-in electric vehicles (PEVs) into the agencies’ fleets. Individual observations of these selected vehicles provide the basis for recommendations related to electric vehicle adoption and whether a battery electric vehicle (BEV) or plug-in hybrid electric vehicle (PHEV) (collectively plug-in electric vehicles, or PEVs) can fulfill the mission requirements.

  8. AVTA Federal Fleet PEV Readiness Data Logging and Characterization Study for National Institute of Health

    Energy Technology Data Exchange (ETDEWEB)

    Schey, Stephen [Idaho National Lab. (INL), Idaho Falls, ID (United States); Francfort, Jim [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2014-11-01

    This report focuses on the National Institute of Health (NIH) fleet to identify daily operational characteristics of select vehicles and report findings on vehicle and mission characterizations to support the successful introduction of plug-in electric vehicles (PEVs) into the agencies’ fleets. Individual observations of these selected vehicles provide the basis for recommendations related to electric vehicle adoption and whether a battery electric vehicle (BEV) or plug-in hybrid electric vehicle (PHEV) (collectively plug-in electric vehicles, or PEVs) can fulfill the mission requirements.

  9. AVTA Federal Fleet PEV Readiness Data Logging and Characterization Study for NASA Glenn Research Center

    Energy Technology Data Exchange (ETDEWEB)

    Schey, Stephen [Idaho National Lab. (INL), Idaho Falls, ID (United States); Francfort, Jim [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2014-10-01

    The Advanced Vehicle Testing Activity’s study seeks to collect and evaluate data to validate the utilization of advanced plug-in electric vehicle (PEV) transportation. This report focuses on the NASA Glenn Research Center (GRC) fleet to identify daily operational characteristics of select vehicles and report findings on vehicle and mission characterizations to support the successful introduction of PEVs into the agencies’ fleets. Individual observations of these selected vehicles provide the basis for recommendations related to electric vehicle adoption and whether a battery electric vehicle or plug-in hybrid electric vehicle (collectively referred to as PEVs) can fulfill the mission requirements.

  10. On the road : non-fossil fuel deployment for the public bus fleet of Sweden

    OpenAIRE

    Xylia, Maria; Silveira, Semida

    2015-01-01

    The public transport sector in Sweden has set a target to run 90% of its total vehicle-kilometers on renewable fuels by 2020, and double its market share in the long term. The focus of this paper is the adoption of renewable fuels in public bus fleets. Data for all 21 Swedish counties were gathered and analyzed, mapping the bus fleets’ condition in relation to renewable fuel deployment, CO2 emissions and energy efficiency. The main factors affecting fuel choices in the bus fleets were investi...

  11. Taking an Alternative Route: A guide for fleet operators and individual owners using alternative fuels in cars and trucks

    International Nuclear Information System (INIS)

    LaRocque, T.

    2001-01-01

    Taking an Alternative Route is a 30-page guide for fleet managers and individual owners on using alternative fuels in cars and trucks. Discussed in detail are all fuels authorized for federal credits under the Energy Policy Act of 1992 (EPAct). The publication informs federal and state fleet managers about how to comply with EPAct, and provides information about the Clean Air Act Amendments

  12. CoalFleet for tomorrow. An industry initiative to accelerate the deployment of advanced coal-based generation plants

    Energy Technology Data Exchange (ETDEWEB)

    Parkes, J.; Holt, N.; Phillips, J. [Electric Power Research Institute (United States)

    2006-07-01

    The industry initiative 'CoalFleet for tomorrow' was launched in November 2004 to accelerate the deployment and commercialization of clean, efficient, advanced coal power systems. This paper discusses the structure of CoalFleet and its strategy for reducing the cost, leadtime and risk of deploying advanced coal technologies such as combined-cycle power plants. 6 figs.

  13. EVALUACIÓN DE LAS COMPETENCIAS LABORALES Y SU INFLUENCIA EN LA CALIDAD DEL SERVICIO DEL HOSPITAL “DR. MARIO MUÑOZ MONROY”

    Directory of Open Access Journals (Sweden)

    Lázaro Quintana Tápanes

    2009-02-01

    Full Text Available

    En el artículo se identifican, evalúan las competencias laborales a nivel organizacional, de procesos clínicos – quirúrgicos y se determina su influencia en la calidad del servicio del Hospital “Dr. Mario Muñoz Monroy”. Se elaboran los perfiles de competencias correspondientes y se calcula el indicador desviación competencial, que permite conocer el comportamiento de las competencias en la comparación entre el estado deseado y actual, en los niveles jerárquicos antes mencionados. Se emplean diferentes métodos y técnicas como: análisis documental, entrevista, cuestionario, pruebas de conocimientos, observación, dinámica de grupo, método delphi, análisis-síntesis, inducción-deducción y el paquete estadístico SPSS versión 12.0. La muestra seleccionada está compuesta por clientes internos (médicos, enfermeros y por clientes externos (pacientes-familiares.

    Se identifican las competencias menos desarrolladas y se proponen estrategias a implementar por la entidad que permitan mejorar la calidad a través de las competencias laborales.

  14. 5'-Phospho-RNA Acceptor Specificity of GDP Polyribonucleotidyltransferase of Vesicular Stomatitis Virus in mRNA Capping.

    Science.gov (United States)

    Ogino, Minako; Ogino, Tomoaki

    2017-03-15

    The GDP polyribonucleotidyltransferase (PRNTase) domain of the multifunctional L protein of rhabdoviruses, such as vesicular stomatitis virus (VSV) and rabies virus, catalyzes the transfer of 5'-phospho-RNA (pRNA) from 5'-triphospho-RNA (pppRNA) to GDP via a covalent enzyme-pRNA intermediate to generate a 5'-cap structure (GpppA). Here, using an improved oligo-RNA capping assay with the VSV L protein, we showed that the Michaelis constants for GDP and pppAACAG (VSV mRNA-start sequence) are 0.03 and 0.4 μM, respectively. A competition assay between GDP and GDP analogues in the GpppA formation and pRNA transfer assay using GDP analogues as pRNA acceptors indicated that the PRNTase domain recognizes the C-2-amino group, but not the C-6-oxo group, N-1-hydrogen, or N-7-nitrogen, of GDP for the cap formation. 2,6-Diaminopurine-riboside (DAP), 7-deazaguanosine (7-deaza-G), and 7-methylguanosine (m 7 G) diphosphates efficiently accepted pRNA, resulting in the formation of DAPpppA, 7-deaza-GpppA, and m 7 GpppA (cap 0), respectively. Furthermore, either the 2'- or 3'-hydroxyl group of GDP was found to be required for efficient pRNA transfer. A 5'-diphosphate form of antiviral ribavirin weakly inhibited the GpppA formation but did not act as a pRNA acceptor. These results indicate that the PRNTase domain has a unique guanosine-binding mode different from that of eukaryotic mRNA capping enzyme, guanylyltransferase. IMPORTANCE mRNAs of nonsegmented negative-strand (NNS) RNA viruses, such as VSV, possess a fully methylated cap structure, which is required for mRNA stability, efficient translation, and evasion of antiviral innate immunity in host cells. GDP polyribonucleotidyltransferase (PRNTase) is an unconventional mRNA capping enzyme of NNS RNA viruses that is distinct from the eukaryotic mRNA capping enzyme, guanylyltransferase. In this study, we studied the pRNA acceptor specificity of VSV PRNTase using various GDP analogues and identified chemical groups of GDP as

  15. 5′-Phospho-RNA Acceptor Specificity of GDP Polyribonucleotidyltransferase of Vesicular Stomatitis Virus in mRNA Capping

    Science.gov (United States)

    Ogino, Minako

    2017-01-01

    ABSTRACT The GDP polyribonucleotidyltransferase (PRNTase) domain of the multifunctional L protein of rhabdoviruses, such as vesicular stomatitis virus (VSV) and rabies virus, catalyzes the transfer of 5′-phospho-RNA (pRNA) from 5′-triphospho-RNA (pppRNA) to GDP via a covalent enzyme-pRNA intermediate to generate a 5′-cap structure (GpppA). Here, using an improved oligo-RNA capping assay with the VSV L protein, we showed that the Michaelis constants for GDP and pppAACAG (VSV mRNA-start sequence) are 0.03 and 0.4 μM, respectively. A competition assay between GDP and GDP analogues in the GpppA formation and pRNA transfer assay using GDP analogues as pRNA acceptors indicated that the PRNTase domain recognizes the C-2-amino group, but not the C-6-oxo group, N-1-hydrogen, or N-7-nitrogen, of GDP for the cap formation. 2,6-Diaminopurine-riboside (DAP), 7-deazaguanosine (7-deaza-G), and 7-methylguanosine (m7G) diphosphates efficiently accepted pRNA, resulting in the formation of DAPpppA, 7-deaza-GpppA, and m7GpppA (cap 0), respectively. Furthermore, either the 2′- or 3′-hydroxyl group of GDP was found to be required for efficient pRNA transfer. A 5′-diphosphate form of antiviral ribavirin weakly inhibited the GpppA formation but did not act as a pRNA acceptor. These results indicate that the PRNTase domain has a unique guanosine-binding mode different from that of eukaryotic mRNA capping enzyme, guanylyltransferase. IMPORTANCE mRNAs of nonsegmented negative-strand (NNS) RNA viruses, such as VSV, possess a fully methylated cap structure, which is required for mRNA stability, efficient translation, and evasion of antiviral innate immunity in host cells. GDP polyribonucleotidyltransferase (PRNTase) is an unconventional mRNA capping enzyme of NNS RNA viruses that is distinct from the eukaryotic mRNA capping enzyme, guanylyltransferase. In this study, we studied the pRNA acceptor specificity of VSV PRNTase using various GDP analogues and identified chemical groups

  16. Using Discrete Event Simulation To Analyze Personnel Requirements For The Malaysian Armys New Utility Helicopter Fleet

    Science.gov (United States)

    2016-06-01

    HELICOPTER FLEET Hasnan bin Mohamad Rais Major, Malaysian Army B.S., University Technology of Malaysia , 2000 Submitted in partial...HELICOPTER MAINTENANCE POLICY B. The objective of MAA helicopter maintenance activities is to preserve helicopter safety and mission reliability to

  17. INL Fleet Vehicle Characterization Study for the U.S. Department of Navy

    Energy Technology Data Exchange (ETDEWEB)

    Bennett, Brion Dale [Idaho National Lab. (INL), Idaho Falls, ID (United States); Francfort, James Edward [Idaho National Lab. (INL), Idaho Falls, ID (United States); Smart, John Galloway [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-09-01

    Battelle Energy Alliance, LLC, managing and operating contractor for the U.S. Department of Energy’s Idaho National Laboratory, is the lead laboratory for U.S. Department of Energy Advanced Vehicle Testing. Battelle Energy Alliance, LLC collected and evaluated data on federal fleet operations as part of the Advanced Vehicle Testing Activity’s Federal Fleet Vehicle Data Logging and Characterization Study. The Advanced Vehicle Testing Activity’s study seeks to collect and evaluate data to validate use of advanced plug-in electric vehicle (PEV) transportation. This report focuses on US Department of Navy's fleet to identify daily operational characteristics of select vehicles and report findings on vehicle and mission characterizations to support the successful introduction of PEVs into the agency’s fleets. Individual observations of these selected vehicles provide the basis for recommendations related to electric vehicle adoption and whether a battery electric vehicle or plug-in hybrid electric vehicle (collectively referred to as PEVs) can fulfill the mission requirements.

  18. 77 FR 14482 - Petroleum Reduction and Alternative Fuel Consumption Requirements for Federal Fleets

    Science.gov (United States)

    2012-03-12

    ...-road or non-road vehicle. \\2\\ The definition of the term ``low-speed electric vehicle,'' as used... alternative fuel used in Federal fleet motor vehicles. This report also would include the alternative fuel...-- (1) a motor vehicle that operates solely on alternative fuel; or (2) a low-speed electric vehicle. (g...

  19. New Trends in Robotics for Agriculture: Integration and Assessment of a Real Fleet of Robots

    Science.gov (United States)

    Gonzalez-de-Soto, Mariano; Pajares, Gonzalo

    2014-01-01

    Computer-based sensors and actuators such as global positioning systems, machine vision, and laser-based sensors have progressively been incorporated into mobile robots with the aim of configuring autonomous systems capable of shifting operator activities in agricultural tasks. However, the incorporation of many electronic systems into a robot impairs its reliability and increases its cost. Hardware minimization, as well as software minimization and ease of integration, is essential to obtain feasible robotic systems. A step forward in the application of automatic equipment in agriculture is the use of fleets of robots, in which a number of specialized robots collaborate to accomplish one or several agricultural tasks. This paper strives to develop a system architecture for both individual robots and robots working in fleets to improve reliability, decrease complexity and costs, and permit the integration of software from different developers. Several solutions are studied, from a fully distributed to a whole integrated architecture in which a central computer runs all processes. This work also studies diverse topologies for controlling fleets of robots and advances other prospective topologies. The architecture presented in this paper is being successfully applied in the RHEA fleet, which comprises three ground mobile units based on a commercial tractor chassis. PMID:25143976

  20. Something To Rely On : The Influence of Stable and Fleeting Drivers on Moral Behavior

    NARCIS (Netherlands)

    G.G. van Houwelingen (Gijs)

    2015-01-01

    markdownabstract__Abstract__ In virtually any situation we are bound to encounter short-lived influences that lure us to act in a certain way. The influence of such ‘fleeting drivers’ may or may not be in line our long-term goals and commitments (‘stable drivers’). Moral behaviour in particular

  1. Guidelines for the Establishment of a Model Neighborhood Electric Vehicle (NEV) Fleet

    Energy Technology Data Exchange (ETDEWEB)

    Roberta Brayer; Donald Karner; Kevin Morrow; James Francfort

    2006-06-01

    The U.S. Department of Energy’s Advanced Vehicle Testing Activity tests neighborhood electric vehicles (NEVs) in both track and fleet testing environments. NEVs, which are also known as low speed vehicles, are light-duty vehicles with top speeds of between 20 and 25 mph, and total gross vehicle weights of approximately 2,000 pounds or less. NEVs have been found to be very viable alternatives to internal combustion engine vehicles based on their low operating costs. However, special charging infrastructure is usually necessary for successful NEV fleet deployment. Maintenance requirements are also unique to NEVs, especially if flooded lead acid batteries are used as they have watering requirements that require training, personnel protection equipment, and adherence to maintenance schedules. This report provides guidelines for fleet managers to follow in order to successfully introduce and operate NEVs in fleet environments. This report is based on the NEV testing and operational experience of personnel from the Advanced Vehicle Testing Activity, Electric Transportation Applications, and the Idaho National Laboratory.

  2. Aging Military Aircraft Landscape : A Case for End-of-Life Fleet Optimization

    NARCIS (Netherlands)

    Newcamp, J.M.; Verhagen, W.J.C.; Curran, Ricky

    2016-01-01

    Military aircraft fleets are continuing to age despite increased structural integrity concerns and rising maintenance costs. Aircraft are not being replaced or retired in large numbers but are instead having their lives extended beyond their original design service lives. Because aging aircraft cost

  3. Towards the implementation of an integrated ecosystem fleet-based management of European fisheries

    NARCIS (Netherlands)

    Gascuel, D.; Merino, G.; Döring, R.D.; Druon, D.N.; Goti, L.; Guénette, S.; Macher, C.; Soma, K.; Travers-Trolet, M.; Mackinson, S.

    2012-01-01

    Using the Celtic Sea and the North Sea as case studies, the fleet-based approach is shown to be the pathway to implement an effective ecosystem approach to fisheries management (EAFM) in European seas. First, a diagnostic on the health of each ecosystem is proposed based on the reconstruction of

  4. 41 CFR 102-34.300 - How do we dispose of a domestic fleet motor vehicle?

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false How do we dispose of a domestic fleet motor vehicle? 102-34.300 Section 102-34.300 Public Contracts and Property Management Federal Property Management Regulations System (Continued) FEDERAL MANAGEMENT REGULATION PERSONAL PROPERTY...

  5. 75 FR 45055 - Security Zone; 2010 Seattle Seafair Fleet Week Moving Vessels, Puget Sound, WA

    Science.gov (United States)

    2010-08-02

    ...-AA87 Security Zone; 2010 Seattle Seafair Fleet Week Moving Vessels, Puget Sound, WA AGENCY: Coast Guard... (NCSM 710) which include all waters within 100 yards from the vessels while underway in the Puget Sound... authorized by the COTP, Puget Sound or Designated Representative. DATES: This rule is effective from 8 a.m...

  6. 77 FR 44475 - Security Zones; Seattle's Seafair Fleet Week Moving Vessels, Puget Sound, WA

    Science.gov (United States)

    2012-07-30

    ...-AA87 Security Zones; Seattle's Seafair Fleet Week Moving Vessels, Puget Sound, WA AGENCY: Coast Guard... temporary rule, call or email Lieutenant Junior Grade Anthony P. LaBoy, Sector Puget Sound, Waterways Management Division, U.S. Coast Guard; telephone 206-217-6323, email SectorPugetSound[email protected] . If you...

  7. Modeling, simulation & optimization of the landing craft air cushion fleet readiness.

    Energy Technology Data Exchange (ETDEWEB)

    Engi, Dennis

    2006-10-01

    The Landing Craft Air Cushion is a high-speed, over-the-beach, fully amphibious landing craft capable of carrying a 60-75 ton payload. The LCAC fleet can serve to transport weapons systems, equipment, cargo and personnel from ship to shore and across the beach. This transport system is an integral part of our military arsenal and, as such, its readiness is an important consideration for our national security. Further, the best way to expend financial resources that have been allocated to maintain this fleet is a critical Issue. There is a clear coupling between the measure of Fleet Readiness as defined by the customer for this project and the information that is provided by Sandia's ProOpta methodology. Further, there is a richness in the data that provides even more value to the analyst. This report provides an analytic framework for understanding the connection between Fleet Readiness and the output provided by Sandia's ProOpta software. Further, this report highlights valuable information that can also be made available using the ProOpta output and concepts from basic probability theory. Finally, enabling assumptions along with areas that warrant consideration for further study are identified.

  8. Multi-objective decision-making model based on CBM for an aircraft fleet

    Science.gov (United States)

    Luo, Bin; Lin, Lin

    2018-04-01

    Modern production management patterns, in which multi-unit (e.g., a fleet of aircrafts) are managed in a holistic manner, have brought new challenges for multi-unit maintenance decision making. To schedule a good maintenance plan, not only does the individual machine maintenance have to be considered, but also the maintenance of the other individuals have to be taken into account. Since most condition-based maintenance researches for aircraft focused on solely reducing maintenance cost or maximizing the availability of single aircraft, as well as considering that seldom researches concentrated on both the two objectives: minimizing cost and maximizing the availability of a fleet (total number of available aircraft in fleet), a multi-objective decision-making model based on condition-based maintenance concentrated both on the above two objectives is established. Furthermore, in consideration of the decision maker may prefer providing the final optimal result in the form of discrete intervals instead of a set of points (non-dominated solutions) in real decision-making problem, a novel multi-objective optimization method based on support vector regression is proposed to solve the above multi-objective decision-making model. Finally, a case study regarding a fleet is conducted, with the results proving that the approach efficiently generates outcomes that meet the schedule requirements.

  9. 77 FR 21448 - Security Zone; 2012 Fleet Week, Port Everglades, Fort Lauderdale, FL

    Science.gov (United States)

    2012-04-10

    ... Environmental Health Risks and Safety Risks. This rule is not an economically significant rule and does not create an environmental risk to health or risk to safety that may disproportionately affect children...-AA87 Security Zone; 2012 Fleet Week, Port Everglades, Fort Lauderdale, FL AGENCY: Coast Guard, DHS...

  10. New Trends in Robotics for Agriculture: Integration and Assessment of a Real Fleet of Robots

    Directory of Open Access Journals (Sweden)

    Luis Emmi

    2014-01-01

    Full Text Available Computer-based sensors and actuators such as global positioning systems, machine vision, and laser-based sensors have progressively been incorporated into mobile robots with the aim of configuring autonomous systems capable of shifting operator activities in agricultural tasks. However, the incorporation of many electronic systems into a robot impairs its reliability and increases its cost. Hardware minimization, as well as software minimization and ease of integration, is essential to obtain feasible robotic systems. A step forward in the application of automatic equipment in agriculture is the use of fleets of robots, in which a number of specialized robots collaborate to accomplish one or several agricultural tasks. This paper strives to develop a system architecture for both individual robots and robots working in fleets to improve reliability, decrease complexity and costs, and permit the integration of software from different developers. Several solutions are studied, from a fully distributed to a whole integrated architecture in which a central computer runs all processes. This work also studies diverse topologies for controlling fleets of robots and advances other prospective topologies. The architecture presented in this paper is being successfully applied in the RHEA fleet, which comprises three ground mobile units based on a commercial tractor chassis.

  11. Alternative Fuels and Advanced Vehicles: Resources for Fleet Managers (Clean Cities) (Presentation)

    Energy Technology Data Exchange (ETDEWEB)

    Brennan, A.

    2011-04-01

    A discussion of the tools and resources on the Clean Cities, Alternative Fuels and Advanced Vehicles Data Center, and the FuelEconomy.gov Web sites that can help vehicle fleet managers make informed decisions about implementing strategies to reduce gasoline and diesel fuel use.

  12. Fleet Conversion in Local Government: Determinants of Driver Fuel Choice for Bi-Fuel Vehicles

    Science.gov (United States)

    Johns, Kimberly D.; Khovanova, Kseniya M.; Welch, Eric W.

    2009-01-01

    This study evaluates the conversion of one local government's fleet from gasoline to bi-fuel E-85, compressed natural gas, and liquid propane gas powered vehicles at the midpoint of a 10-year conversion plan. This study employs a behavioral model based on the theory of reasoned action to explore factors that influence an individual's perceived and…

  13. Fleet DNA Phase 1 Refinement & Phase 2 Implementation; NREL (National Renewable Energy Laboratory)

    Energy Technology Data Exchange (ETDEWEB)

    Kelly, Kenneth; Duran, Adam

    2015-06-11

    Fleet DNA acts as a secure data warehouse for medium- and heavy-duty vehicle data. It demonstrates that vehicle drive cycle data can be collected and stored for large-scale analysis and modeling applications. The data serve as a real-world data source for model development and validation. Storage of the results of past/present/future data collection efforts improves analysis efficiency through pooling of shared data and provides the opportunity for 'big data' type analyses. Fleet DNA shows it is possible to develop a common database structure that can store/analyze/report on data sourced from multiple parties, each with unique data formats/types. Data filtration and normalization algorithms developed for the project allow for a wide range of data types and inputs, expanding the project’s potential. Fleet DNA demonstrates the power of integrating Big Data with existing and future tools and analyses: it provides an enhanced understanding and education of users, users can explore greenhouse gases and economic opportunities via AFLEET and ADOPT modeling, drive cycles can be characterized and visualized using DRIVE, high-level vehicle modeling can be performed using real-world drive cycles via FASTSim, and data reporting through Fleet DNA Phase 1 and 2 websites provides external users access to analysis results and gives the opportunity to explore on their own.

  14. New trends in robotics for agriculture: integration and assessment of a real fleet of robots.

    Science.gov (United States)

    Emmi, Luis; Gonzalez-de-Soto, Mariano; Pajares, Gonzalo; Gonzalez-de-Santos, Pablo

    2014-01-01

    Computer-based sensors and actuators such as global positioning systems, machine vision, and laser-based sensors have progressively been incorporated into mobile robots with the aim of configuring autonomous systems capable of shifting operator activities in agricultural tasks. However, the incorporation of many electronic systems into a robot impairs its reliability and increases its cost. Hardware minimization, as well as software minimization and ease of integration, is essential to obtain feasible robotic systems. A step forward in the application of automatic equipment in agriculture is the use of fleets of robots, in which a number of specialized robots collaborate to accomplish one or several agricultural tasks. This paper strives to develop a system architecture for both individual robots and robots working in fleets to improve reliability, decrease complexity and costs, and permit the integration of software from different developers. Several solutions are studied, from a fully distributed to a whole integrated architecture in which a central computer runs all processes. This work also studies diverse topologies for controlling fleets of robots and advances other prospective topologies. The architecture presented in this paper is being successfully applied in the RHEA fleet, which comprises three ground mobile units based on a commercial tractor chassis.

  15. 40 CFR 86.1865-12 - How to comply with the fleet average CO2 standards.

    Science.gov (United States)

    2010-07-01

    ... efficiency credits earned according to the provisions of § 86.1866-12(c); (iii) Off-cycle technology credits... change Selective Enforcement Auditing or in-use testing failures from a failure to a non-failure. The.... (l) Maintenance of records and submittal of information relevant to compliance with fleet average CO...

  16. The choice of leasing companies for automobile fleet updating on the basis of hierarchies analysis method

    OpenAIRE

    Dorohov, А.

    2007-01-01

    The basic criteria of leasing companies choice by the transport enterprises for automobile fleet updating such as terms of financing, size of advance, assortment time of existence at the market, have been determined. The determination of the best leasing company according to these parameters on the basis of hierarchies analysis method has been offered.

  17. 76 FR 40617 - Security Zone; 2011 Seattle Seafair Fleet Week Moving Vessels, Puget Sound, Washington

    Science.gov (United States)

    2011-07-11

    ... better evaluate its effects on them and participate in the rulemaking process. Small businesses may send... DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 165 [Docket No. USCG-2011-0505] RIN 1625-AA87 Security Zone; 2011 Seattle Seafair Fleet Week Moving Vessels, Puget Sound, Washington AGENCY...

  18. Status of the French photovoltaic fleet on the 30 September 2011

    International Nuclear Information System (INIS)

    2012-01-01

    This publication first present some key data regarding photovoltaic energy production in France (connected and to be connected), and then presents the photovoltaic fleet (geographical distribution, installed power in metropolitan France, Corsica and ultramarine territories). It gives an overview of the evolution of connection request and queuing management in France and at the regional level

  19. Alternative fuels for vehicles fleet demonstration program. Final report, volume 2: Appendices

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-06-01

    The Alternative Fuels for Vehicles Fleet Demonstration Program (AFV-FDP) was a multiyear effort to collect technical data for use in determining the costs and benefits of alternative-fuel vehicles (AFVs) in typical applications in New York State. This report, Volume 2, includes 13 appendices to Volume 1 that expand upon issues raised therein. Volume 1 provides: (1) Information about the purpose and scope of the AFV-FDP; (2) A summary of AFV-FDP findings organized on the basis of vehicle type and fuel type; (3) A short review of the status of AFV technology development, including examples of companies in the State that are active in developing AFVs and AFV components; and (4) A brief overview of the status of AFV deployment in the State. Volume 3 provides expanded reporting of AFV-FDP technical details, including the complete texts of the brochure Garage Guidelines for Alternative Fuels and the technical report Fleet Experience Survey Report, plus an extensive glossary of AFV terminology. The appendices cover a wide range of issues including: emissions regulations in New York State; production and health effects of ozone; vehicle emissions and control systems; emissions from heavy-duty engines; reformulated gasoline; greenhouse gases; production and characteristics of alternative fuels; the Energy Policy Act of 1992; the Clean Fuel Fleet Program; garage design guidelines for alternative fuels; surveys of fleet managers using alternative fuels; taxes on conventional and alternative fuels; and zero-emission vehicle technology.

  20. Off-wing fleet maintenance study of a CFM56-3B turbofan engine ...

    African Journals Online (AJOL)

    An off wing fleet maintenance study of the CFM56-3B Turbofan engine that propels the Boeing 737-300 aircraft is presented. The engine performance and deteriorating behavior was modeled with a view to estimate the creep life consumption and operating severity. The predicted severity factor of each degradation was ...

  1. Fleet deployment, network design and hub location of liner shipping companies

    DEFF Research Database (Denmark)

    Gelareh, Shahin; Pisinger, David

    2011-01-01

    A mixed integer linear programming formulation is proposed for the simultaneous design of network and fleet deployment of a deep-sea liner service provider. The underlying network design problem is based on a 4-index (5-index by considering capacity type) formulation of the hub location problem...

  2. Impact of environmental constraints and aircraft technology on airline fleet composition

    Science.gov (United States)

    Moolchandani, Kushal A.

    This thesis models an airline's decisions about fleet evolution in order to maintain economic and regulatory viability. The aim is to analyze the fleet evolution under different scenarios of environmental policy and technology availability in order to suggest an optimal fleet under each case. An understanding of the effect of aircraft technologies, fleet size and age distribution, and operational procedures on airline performance may improve the quality of policies to achieve environmental goals. Additionally, the effect of decisions about fleet evolution on air travel is assessed as the change in market demand and profits of an abstracted, benevolent monopolist airline. Attention to the environmental impact of aviation has grown, and this has prompted several organizations such as ICAO (and, in response, NASA) to establish emissions reduction targets to reduce aviation's global climate impact. The introduction of new technology, change in operational procedures, etc. are some of the proposed means to achieve these targets. Of these, this thesis studies the efficacy of implementation of environmental policies in form of emissions constraints as a means to achieve these goals and assesses their impact on an airline's fleet evolution and technology use (along with resulting effects on air travel demand). All studies in this thesis are conducted using the Fleet-level Environmental Evaluation Tool (FLEET), a NASA sponsored simulation tool developed at Purdue University. This tool models airline operational decisions via a resource allocation problem and uses a system dynamics type approach to mimic airline economics, their decisions regarding retirement and acquisition of aircraft and evolution of market demand in response to the economic conditions. The development of an aircraft acquisition model for FLEET is a significant contribution of the author. Further, the author conducted a study of various environmental policies using FLEET. Studies introduce constraints on

  3. Caffeine decreases phospho-Chk1 (Ser317) and increases mitotic cells with cyclin B1 and caspase 3 in tumors from UVB-treated mice.

    Science.gov (United States)

    Lu, Yao-Ping; Lou, You-Rong; Peng, Qing-Yun; Nghiem, Paul; Conney, Allan H

    2011-07-01

    Oral administration of caffeine to mice inhibits UVB-induced carcinogenesis, and these results are paralleled by epidemiology studies indicating that caffeinated coffee and tea intake (but not decaffeinated beverage intake) is associated with decreased incidence of nonmelanoma skin cancer. Topical applications of caffeine to the skin of SKH-1 mice that had previously been treated with UVB inhibited subsequent skin tumor development and stimulated apoptosis in tumors but not in nontumor areas of the epidermis. This study sought to determine the basis of these differential effects on tumor versus nontumor sites that can be induced by caffeine, long after all UVB treatment has ceased. The activation status of the ATR/Chk1 pathway in UVB-induced tumors and uninvolved skin was determined by quantitating phospho-Chk1 (Ser317) and induction of lethal mitosis in vivo in the presence and absence of topical caffeine treatment. In the absence of caffeine, we found that UVB-induced tumors often had islands of phospho-Chk1 (Ser317) staining cells that were not present in nontumor areas of the epidermis. Treatment of mice with topical caffeine significantly diminished phospho-Chk1 (Ser317) staining and increased the number of mitotic cells that expressed cyclin B1 and caspase 3 in tumors, consistent with caffeine-induced lethal mitosis selectively in tumors. We hypothesize that compared with adjacent uninvolved skin, UVB-induced skin tumors have elevated activation of, and dependence on, the ATR/Chk1 pathway long after UVB exposure has ceased and that caffeine can induce apoptosis selectively in tumors by inhibiting this pathway and promoting lethal mitosis.

  4. Deletion mutants of the Escherichia coli K-12 mannitol permease: dissection of transport-phosphorylation, phospho-exchange, and mannitol-binding activities.

    Science.gov (United States)

    Grisafi, P L; Scholle, A; Sugiyama, J; Briggs, C; Jacobson, G R; Lengeler, J W

    1989-05-01

    We have constructed a series of deletion mutations of the cloned Escherichia coli K-12 mtlA gene, which encodes the mannitol-specific enzyme II of the phosphoenolpyruvate (PEP)-dependent carbohydrate phosphotransferase system. This membrane-bound permease consists of 637 amino acid residues and is responsible for the concomitant transport and phosphorylation of D-mannitol in E. coli. Deletions into the 3' end of mtlA were constructed by exonuclease III digestion. Restriction mapping of the resultant plasmids identified several classes of deletions that lacked approximately 5% to more than 75% of the gene. Immunoblotting experiments revealed that many of these plasmids expressed proteins within the size range predicted by the restriction analyses, and all of these proteins were membrane localized, which demonstrated that none of the C-terminal half of the permease is required for membrane insertion. Functional analyses of the deletion proteins, expressed in an E. coli strain deleted for the chromosomal copy of mtlA, showed that all but one of the strains containing confirmed deletions were inactive in transport and PEP-dependent phosphorylation of mannitol, but deletions removing up to at least 117 amino acid residues from the C terminus of the permease were still active in catalyzing phospho exchange between mannitol 1-phosphate and mannitol. A deletion protein that lacked 240 residues from the C terminus of the permease was inactive in phospho exchange but still bound mannitol with high affinity. These experiments localize sites important for transport and PEP-dependent phosphorylation to the extreme C terminus of the mannitol permease, sites important for phospho exchange to between residues 377 and 519, and sites necessary for mannitol binding to the N-terminal 60% of the molecule. The results are discussed with respect to the fact that the mannitol permease consists of structurally independent N- and C-terminal domains.

  5. Optimization of transit bus fleet's life cycle assessment impacts with alternative fuel options

    International Nuclear Information System (INIS)

    Ercan, Tolga; Zhao, Yang; Tatari, Omer; Pazour, Jennifer A.

    2015-01-01

    Public transportation is one of the most promising transportation modes to reduce the environmental emissions of the transportation sector in the U.S. In order to mitigate the environmental impacts brought by the transit bus system, new energy buses are introduced into the vehicle market. The goal of this study is to find an optimal bus fleet combination for different driving conditions to minimize life cycle cost, greenhouse gas emissions, and conventional air pollutant emission impacts. For this purpose, a Multi-Objective Linear Programming approach is used to select the optimum bus fleet combinations. Given different weight scenarios, this method could effectively provide solutions for decision makers with various budget constraints or emission reduction requirements. The results indicate that in heavily congested driving cycles such as the Manhattan area, the battery electric bus is the dominant vehicle type, while the hybrid bus has more balanced performances in most scenarios because of its lower initial investment comparing to battery electric buses. Petroleum powered buses have seldom been selected by the model. The trade-off analysis shows that the overall greenhouse gas impact performance is sensitive to the life cycle cost after certain points, which could provide valuable information for the bus fleet combination planning. - Highlights: • Hybrid-Life Cycle Assessment analysis approach for transit bus operations. • Optimizing the economic and sustainability impacts of transit bus fleet operation. • CO 2 emissions and other air pollutants related health and environmental damage cost. • Trade-offs between CO 2 emissions and cost of transit bus fleet operation.

  6. Conversion of Syngas-Derived C2+ Mixed Oxygenates to C3-C5 Olefins over ZnxZryOz Mixed Oxides Catalysts

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Colin D.; Lebarbier, Vanessa M.; Flake, Matthew D.; Ramasamy, Karthikeyan K.; Kovarik, Libor; Bowden, Mark E.; Onfroy, Thomas; Dagle, Robert A.

    2016-04-01

    In this study we report on a ZnxZryOz mixed oxide type catalyst capable of converting a syngas-derived C2+ mixed oxygenate feedstock to isobutene-rich olefins. Aqueous model feed comprising of ethanol, acetaldehyde, acetic acid, ethyl acetate, methanol, and propanol was used as representative liquid product derived from a Rh-based mixed oxygenate synthesis catalyst. Greater than 50% carbon yield to C3-C5 mixed olefins was demonstrated when operating at 400-450oC and 1 atm. In order to rationalize formation of the products observed feed components were individually evaluated. Major constituents of the feed mixture (ethanol, acetaldehyde, acetic acid, and ethyl acetate) were found to produce isobutene-rich olefins. C-C coupling was also demonstrated for propanol feedstock - a minor constituent of the mixed oxygenate feed - producing branched C6 olefins, revealing scalability to alcohols higher than ethanol following an analogous reaction pathway. Using ethanol and propanol feed mixtures, cross-coupling reactions produced mixtures of C4, C5, and C6 branched olefins. The presence of H2 in the feed was found to facilitate hydrogenation of the ketone intermediates, thus producing straight chain olefins as byproducts. While activity loss from coking is observed complete catalyst regeneration is achieved by employing mild oxidation. For conversion of the mixed oxygenate feed a Zr/Zn ratio of 2.5 and a reaction temperature of 450oC provides the best balance of stability, activity, and selectivity. X-ray diffraction and scanning transmission electron microscopy analysis reveals the presence of primarily cubic phase ZrO2 and a minor amount of the monoclinic phase, with ZnO being highly dispersed in the lattice. The presence of ZnO appears to stabilize the cubic phase resulting in less monoclinic phase as the ZnO concentration increases. Infrared spectroscopy shows the mixed oxide acid sites are characterized as primarily Lewis type acidity. The direct relationship between

  7. Texto literario y texto fílmico : análisis comparativo-textual de "El invierno en Lisboa, Beltenebros y Plenilunio" de Antonio Muñoz Molina

    OpenAIRE

    Vázquez Naveira, Marta Maria

    2016-01-01

    En la tesis Texto literario y texto jilmico. A nálisis comparativo-textual de El invierno en Lisboa, Beltenebros y Plenilunio de Antonio Muñoz Molina analizamos, desde una perspectiva narratológica y filmica, las tres novelas del escritor y sus adaptaciones cinematográficas. El alcance de las relaciones entre cine y literatura es muy amplio y opera en ambas direcciones. En este trabajo se lleva a cabo un estudio comparativo de los textos con el fin de comprobar si mantienen ...

  8. Blast exposure causes early and persistent aberrant phospho- and cleaved-tau expression in a murine model of mild blast-induced traumatic brain injury.

    Science.gov (United States)

    Huber, Bertrand R; Meabon, James S; Martin, Tobin J; Mourad, Pierre D; Bennett, Raymond; Kraemer, Brian C; Cernak, Ibolja; Petrie, Eric C; Emery, Michael J; Swenson, Erik R; Mayer, Cynthia; Mehic, Edin; Peskind, Elaine R; Cook, David G

    2013-01-01

    Mild traumatic brain injury (mTBI) is considered the 'signature injury' of combat veterans that have served during the wars in Iraq and Afghanistan. This prevalence of mTBI is due in part to the common exposure to high explosive blasts in combat zones. In addition to the threats of blunt impact trauma caused by flying objects and the head itself being propelled against objects, the primary blast overpressure (BOP) generated by high explosives is capable of injuring the brain. Compared to other means of causing TBI, the pathophysiology of mild-to-moderate BOP is less well understood. To study the consequences of BOP exposure in mice, we employed a well-established approach using a compressed gas-driven shock tube that recapitulates battlefield-relevant open-field BOP. We found that 24 hours post-blast a single mild BOP provoked elevation of multiple phospho- and cleaved-tau species in neurons, as well as elevating manganese superoxide-dismutase (MnSOD or SOD2) levels, a cellular response to oxidative stress. In hippocampus, aberrant tau species persisted for at least 30 days post-exposure, while SOD2 levels returned to sham control levels. These findings suggest that elevated phospho- and cleaved-tau species may be among the initiating pathologic processes induced by mild blast exposure. These findings may have important implications for efforts to prevent blast-induced insults to the brain from progressing into long-term neurodegenerative disease processes.

  9. The phospho-occupancy of an atypical SLIMB-binding site on PERIOD that is phosphorylated by DOUBLETIME controls the pace of the clock.

    Science.gov (United States)

    Chiu, Joanna C; Vanselow, Jens T; Kramer, Achim; Edery, Isaac

    2008-07-01

    A common feature of animal circadian clocks is the progressive phosphorylation of PERIOD (PER) proteins, which is highly dependent on casein kinase Idelta/epsilon (CKIdelta/epsilon; termed DOUBLETIME [DBT] in Drosophila) and ultimately leads to the rapid degradation of hyperphosphorylated isoforms via a mechanism involving the F-box protein, beta-TrCP (SLIMB in Drosophila). Here we use the Drosophila melanogaster model system, and show that a key step in controlling the speed of the clock is phosphorylation of an N-terminal Ser (S47) by DBT, which collaborates with other nearby phosphorylated residues to generate a high-affinity atypical SLIMB-binding site on PER. DBT-dependent increases in the phospho-occupancy of S47 are temporally gated, dependent on the centrally located DBT docking site on PER and partially counterbalanced by protein phosphatase activity. We propose that the gradual DBT-mediated phosphorylation of a nonconsensus SLIMB-binding site establishes a temporal threshold for when in a daily cycle the majority of PER proteins are tagged for rapid degradation. Surprisingly, most of the hyperphosphorylation is unrelated to direct effects on PER stability. We also use mass spectrometry to map phosphorylation sites on PER, leading to the identification of a number of "phospho-clusters" that explain several of the classic per mutants.

  10. Crystal structure of Arabidopsis thaliana Dawdle forkhead-associated domain reveals a conserved phospho-threonine recognition cleft for dicer-like 1 binding.

    Science.gov (United States)

    Machida, Satoru; Yuan, Y Adam

    2013-07-01

    Dawdle (DDL) is a microRNA processing protein essential for the development of Arabidopsis. DDL contains a putative nuclear localization signal at its amino-terminus and forkhead-associated (FHA) domain at the carboxyl-terminus. Here, we report the crystal structure of the FHA domain of Arabidopsis Dawdle, determined by multiple-wavelength anomalous dispersion method at 1.7-Å resolution. DDL FHA structure displays a seven-stranded β-sandwich architecture that contains a unique structural motif comprising two long anti-parallel strands. Strikingly, crystal packing of the DDL FHA domain reveals that a glutamate residue from the symmetry-related DDL FHA domain, a structural mimic of the phospho-threonine, is specifically recognized by the structurally conserved phospho-threonine binding cleft. Consistently with the structural observations, co-immuno-precipitation experiments performed in Nicotiana benthamiana show that the DDL FHA domain co-immuno-precipitates with DCL1 fragments containing the predicted pThr+3(Ile/Val/Leu/Asp) motif. Taken together, we count the recognition of the target residue by the canonical binding cleft of the DDL FHA domain as the key molecular event to instate FHA domain-mediated protein-protein interaction in plant miRNA processing.

  11. Food-grade host/vector expression system for Lactobacillus casei based on complementation of plasmid-associated phospho-beta-galactosidase gene lacG.

    Science.gov (United States)

    Takala, T M; Saris, P E J; Tynkkynen, S S H

    2003-01-01

    A new food-grade host/vector system for Lactobacillus casei based on lactose selection was constructed. The wild-type non-starter host Lb. casei strain E utilizes lactose via a plasmid-encoded phosphotransferase system. For food-grade cloning, a stable lactose-deficient mutant was constructed by deleting a 141-bp fragment from the phospho-beta-galactosidase gene lacG via gene replacement. The deletion resulted in an inactive phospho-beta-galactosidase enzyme with an internal in-frame deletion of 47 amino acids. A complementation plasmid was constructed containing a replicon from Lactococcus lactis, the lacG gene from Lb. casei, and the constitutive promoter of pepR for lacG expression from Lb. rhamnosus. The expression of the lacG gene from the resulting food-grade plasmid pLEB600 restored the ability of the lactose-negative mutant strain to grow on lactose to the wild-type level. The vector pLEB600 was used for expression of the proline iminopeptidase gene pepI from Lb. helveticus in Lb. casei. The results show that the food-grade expression system reported in this paper can be used for expression of foreign genes in Lb. casei.

  12. Comparative Analysis of Passenger Traffic Fleets in Asian Cities: Technology, Driving Activities, and Emission

    Science.gov (United States)

    KIM Oanh, N. T.; Huynh, H. V.; Saikawa, E.

    2015-12-01

    The road transport sector is the major emission source of toxic air pollutants and greenhouse gases (GHGs) in large Asian cities. This paper comparatively analyzed on-road passenger traffic fleets (cars, buses, taxis, motorcycles), using local data collected in cities of Bangkok (BKK), Kathmandu, Hanoi, Ho Chi Minh City (HCMC), and Yangon. Surveys were done in 2010-2014 to obtain information on vehicle technology, driving activities (speed, distance, number, and types of starts), traffic density, and fuel characteristics. Large shares of pre-Euro vehicles were still observed, especially for public buses. The most advanced technology was Euro4, which was observed in small shares (<5%) of the personal car fleets in BKK, HCMC, and Yangon. Euro3 was generally the most advanced technology found in other fleets in these cities. Motorcycles (MC) was the most dominant fleet in all cities, except in Yangon, where they were not allowed. Low vehicle speeds, mainly below 25 km/h, were observed for all vehicle types, indicating traffic jams. Natural gas and LPG had considerable shares in BKK and Yangon while for other cities diesel and gasoline were still the two major fuels used in transportation. Running emission factors (EF) of buses and taxis in Kathmandu were considerably higher than other cities due to its hilly topography, low speeds, high mileage, and less advanced vehicle technologies. The number of passenger vehicles per 1000 people were 400-500 in HCMC and Hanoi (mainly by MC) and in BKK (also by cars), moderate in Kathmandu (200) and the lowest in Yangon (40) because of the MC ban. Annual emissions of the passenger fleets were calculated for each city using the International Vehicle Emission (IVE) for 14 species. BC and OC emissions were estimated using their fractions of PM10 emission. Annual emission per capita of toxic air pollutants and GHGs was analyzed. For example, the emission in kg/year/person for CO, VOC, NOx and PM10 in these cities was 24-150 for CO, 0

  13. 77 FR 6080 - Taking and Importing Marine Mammals; U.S. Navy's Atlantic Fleet Active Sonar Training

    Science.gov (United States)

    2012-02-07

    ... research, development, testing, and evaluation (RDT&E) activities to be conducted within the Atlantic Fleet... research activities conducted within the AFAST Study Area under regulations issued on January 22, 2009 (74... [[Page 6082

  14. Causes and consequences of fleet diversity in fisheries: The case of the Norwegian Barents Sea cod fishery

    Directory of Open Access Journals (Sweden)

    Arne Eide

    2016-05-01

    Full Text Available Abstract Fisheries operate under fluctuating environmental conditions, targeting fish stocks that appear in varying densities in different areas, often with abrupt and unexpected local changes. Physical conditions, markets and management regulations constrain vessels in different and varying ways. These factors all contribute to forming the fleet diversity we find in most fisheries. Here, a simulation model of the Northeast Arctic cod fishery is used in order to investigate how this diversity is formed and maintained, assuming rational economic behaviour under varying combined constraints. The study also focuses on how the ability of vessels to find fish influences fleet diversity, profitability, stock development and seasonal profiles of the fishery. Results indicate that an increased ability to target the most profitable fishing grounds may influence fleet diversity positively or negatively, depending on overall exploitation level. High exploitation rates also increase the temporal fluctuations in fleet diversity and profits, which are amplified as the fish-finding ability increases.

  15. Reducing the fuel use and greenhouse gas emissions of the US vehicle fleet

    International Nuclear Information System (INIS)

    Bandivadekar, Anup; Cheah, Lynette; Evans, Christopher; Groode, Tiffany; Heywood, John; Kasseris, Emmanuel; Kromer, Matthew; Weiss, Malcolm

    2008-01-01

    The unrelenting increase in the consumption of oil in the US light-duty vehicle fleet (cars and light trucks) presents an extremely challenging energy and environmental problem. A variety of propulsion technologies and fuels have the promise to reduce petroleum use and greenhouse gas emissions from motor vehicles. Even so, achieving a noticeable reduction on both fronts in the near term will require rapid penetration of these technologies into the vehicle fleet, and not all alternatives can meet both objectives simultaneously. Placing a much greater emphasis on reducing fuel consumption rather than improving vehicle performance can greatly reduce the required market penetration rates. Addressing the vehicle performance-size-fuel consumption trade-off should be the priority for policymakers rather than promoting specific vehicle technologies and fuels

  16. The Electric Fleet Size and Mix Vehicle Routing Problem with Time Windows and Recharging Stations

    DEFF Research Database (Denmark)

    Hiermann, Gerhard; Puchinger, Jakob; Røpke, Stefan

    2016-01-01

    Due to new regulations and further technological progress in the field of electric vehicles, the research community faces the new challenge of incorporating the electric energy based restrictions into vehicle routing problems. One of these restrictions is the limited battery capacity which makes...... detours to recharging stations necessary, thus requiring efficient tour planning mechanisms in order to sustain the competitiveness of electric vehicles compared to conventional vehicles. We introduce the Electric Fleet Size and Mix Vehicle Routing Problem with Time Windows and Recharging Stations (E......-FSMFTW) to model decisions to be made with regards to fleet composition and the actual vehicle routes including the choice of recharging times and locations. The available vehicle types differ in their transport capacity, battery size and acquisition cost. Furthermore, we consider time windows at customer...

  17. Scheduling and location issues in transforming service fleet vehicles to electric vehicles

    DEFF Research Database (Denmark)

    Mirchandani, Pitu; Madsen, Oli B.G.; Adler, Jonathan

    There is much reason to believe that fleets of service vehicles of many organizations will transform their vehicles that utilize alternative fuels which are more sustainable. The electric vehicle is a good candidate for this transformation, especially which “refuels” by exchanging its spent...... batteries with charged ones. This paper discusses the issues that must be addressed if a transit service were to use electric vehicles, principally the issues related to the limited driving range of each electric vehicle’s set of charged batteries and the possible detouring for battery exchanges....... In particular, the paper addresses the optimization and analysis of infrastructure design alternatives dealing with (1) the number of battery-exchange stations, (2) their locations, (3) the recharging capacity and inventory management of batteries at each facility, and (4) routing and scheduling of the fleet...

  18. Electric bus fleet size and mix problem with optimization of charging infrastructure

    DEFF Research Database (Denmark)

    Rogge, Matthias; van der Hurk, Evelien; Larsen, Allan

    2018-01-01

    Battery electric buses are seen as a well-suited technology for the electrification of road-based public transport. However, the transition process from conventional diesel to electric buses faces major hurdles caused by range limitations and required charging times of battery buses. This work...... addresses these constraints and provides a methodology for the cost-optimized planning of depot charging battery bus fleets and their corresponding charging infrastructure. The defined problem covers the scheduling of battery buses, the fleet composition, and the optimization of charging infrastructure...... in a joint process. Vehicle schedule adjustments are monetized and evaluated together with the investment and operational costs of the bus system. The resulting total cost of ownership enables a comparison of technical alternatives on a system level, which makes this approach especially promising...

  19. ENERGY EFFICIENCY AS A CRITERION IN THE VEHICLE FLEET MANAGEMENT PROCESS

    Directory of Open Access Journals (Sweden)

    Davor Vujanović

    2010-01-01

    Full Text Available Transport represents an industry sector with intense energy consumption, the road transport sector within is the dominant subsector. The objective of the research presented in this paper is in defining the activities which applied within road freight transport companies contribute to enhancing vehicles' energy efficiency. Vehicle fleet operation management process effects on fuel consumption decrease have been looked into. Operation parameters that influence vehicle fuel consumption were analysed. In this sense, a survey has been realised in order to evaluate the vehicle load factor impact on the specific fuel consumption. Measures for enhancing vehicle's logistics efficiency have been defined. As a tool for those measures' implementation an algorithm for vehicle fleet operation management was developed which represented a basis for a dedicated software package development for vehicle dispatching process decision support. A set of measures has been recommended and their effects in fuel savings were evaluated.

  20. Waste minimization, recycling and reuse in operations support services fleet maintenance

    International Nuclear Information System (INIS)

    Trego, A.L.

    1994-01-01

    Government regulations and smart business practices demand that organizations dramatically reduce both the type and volume of waste generated by their operations. This article describes successful waste minimization and recycling programs created by the Fleet Maintenance, Operations Support Services Division, Westinghouse Hanford Company. These comprehensive programs have greatly reduced waste formerly produced in maintaining 3,528 government-owned vehicles and nearly 200 emergency power generators at the Hanford Site. The actions are integral to preventing future contamination of the Site as well as to cleaning up the complexity of wastes from almost 50 years of defense production. The results of the Fleet Maintenance programs are impressive, recording cost savings of $290,000 in fiscal year 1993 and $965,000 since 1988

  1. Electric vehicle fleet management in smart grids: A review of services, optimization and control aspects

    DEFF Research Database (Denmark)

    Hu, Junjie; Morais, Hugo; Sousa, Tiago

    2016-01-01

    Electric vehicles can become integral parts of a smart grid, since they are capable of providing valuable services to power systems other than just consuming power. On the transmission system level, electric vehicles are regarded as an important means of balancing the intermittent renewable energy...... resources such as wind power. This is because electric vehicles can be used to absorb the energy during the period of high electricity penetration and feed the electricity back into the grid when the demand is high or in situations of insufficient electricity generation. However, on the distribution system...... and industries. This paper presents a review and classification of methods for smart charging (including power to vehicle and vehicle-to-grid) of electric vehicles for fleet operators. The study firstly presents service relationships between fleet operators and other four actors in smart grids; then, modeling...

  2. Electric Vehicle Preparedness: Task 1, Assessment of Fleet Inventory for Marine Corps Base Camp Lejeune

    Energy Technology Data Exchange (ETDEWEB)

    Schey, Stephen [Idaho National Lab. (INL), Idaho Falls, ID (United States); Francfort, Jim [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-01-01

    Several U.S. Department of Defense-based studies were conducted to identify potential U.S. Department of Defense transportation systems that are strong candidates for introduction or expansion of plug-in electric vehicles (PEVs). Task 1 included a survey of the inventory of non-tactical fleet vehicles at the Marine Corps Base Camp Lejeune (MCBCL) to characterize the fleet. This information and characterization will be used to select vehicles for monitoring that takes place during Task 2. This monitoring involves data logging of vehicle operation in order to identify the vehicle’s mission and travel requirements. Individual observations of these selected vehicles provide the basis for recommendations related to PEV adoption. It also identifies whether a battery electric vehicle or plug-in hybrid electric vehicle (collectively referred to as PEVs) can fulfill the mission requirements and provides observations related to placement of PEV charging infrastructure.

  3. Application of FLEET Velocimetry in the NASA Langley 0.3-meter Transonic Cryogenic Tunnel

    Science.gov (United States)

    Burns, Ross A.; Danehy, Paul M.; Halls, Benjamin R.; Jiang, Naibo

    2015-01-01

    Femtosecond laser electronic excitation and tagging (FLEET) velocimetry is demonstrated in a large-scale transonic cryogenic wind tunnel. Test conditions include total pressures, total temperatures, and Mach numbers ranging from 15 to 58 psia, 200 to 295 K, and 0.2 to 0.75, respectively. Freestream velocity measurements exhibit accuracies within 1 percent and precisions better than 1 m/s. The measured velocities adhere closely to isentropic flow theory over the domain of temperatures and pressures that were tested. Additional velocity measurements are made within the tunnel boundary layer; virtual trajectories traced out by the FLEET signal are indicative of the characteristic turbulent behavior in this region of the flow, where the unsteadiness increases demonstrably as the wall is approached. Mean velocities taken within the boundary layer are in agreement with theoretical velocity profiles, though the fluctuating velocities exhibit a greater deviation from theoretical predictions.

  4. Environmental benefits and economic rationale of expanding the Italian natural gas private car fleet

    International Nuclear Information System (INIS)

    Ballardin, Giorgio

    2005-01-01

    There are several concerns which bring to consider natural gas as a viable alternative to liquid fuels in transport. First, natural gas allows the curbing of global pollution in this steadily growing industry. Indeed, decoupling greenhouse gas emissions from transport growth has become a major issue in tackling climate change. Second, a more extensive use of natural gas would relieve city air quality, which is presently at levels harmful of human health. Nonetheless, this is just one side of the coin. The other side entails building a refuelling station network, and this carries financial requirements. The financing fraction holds a pivotal role in deciding whether natural gas for automotive purposes is an efficient solution. The final aim of this work is, therefore, to compare the natural gas advantages, stemming from avoided global and local emission, with the economic rationale of engaging in supplementary model network investments. A system dynamics model underlies this study's economic reasoning and is taken as reference for quantitative assertions. The model, named CH 4 CleanerAir, contains data referring to two scenarios: a Business As Usual Scenario and an Expansion Scenario. Simulation runs in Expansion Scenario serve to understand how gaseous pollution diminishes and network investments rise when the natural gas-fuelled fleet, as share of the total fleet, increases. Are new infrastructures needs compatible with existing refuelling facilities? What is the extent by which the natural gas-fuelled fleet can actually reduce global and local emission? The scenarios' gap analysis lead to the work's final considerations. The sounder reductions of gaseous pollutants in the last years of the considered time lag make the overall assessment lean towards a positive evaluation of natural gas employment in this industry. The beneficial effects of increasing the natural gas-fuel-led fleet take some time to unfold, but they eventually prevail. This study shows how natural

  5. Assessment of methane-related fuels for automotive fleet vehicles: technical, supply, and economic assessments

    Energy Technology Data Exchange (ETDEWEB)

    1982-02-01

    The use of methane-related fuels, derived from a variety of sources, in highway vehicles is assessed. Methane, as used here, includes natural gas (NG) as well as synthetic natural gas (SNG). Methanol is included because it can be produced from NG or the same resources as SNG, and because it is a liquid fuel at normal ambient conditions. Technological, operational, efficiency, petroleum displacement, supply, safety, and economic issues are analyzed. In principle, both NG and methanol allow more efficient engine operation than gasoline. In practice, engines are at present rarely optimized for NG and methanol. On the basis of energy expended from resource extraction to end use, only optimized LNG vehicles are more efficient than their gasoline counterparts. By 1985, up to 16% of total petroleum-based highway vehicle fuel could be displaced by large fleets with central NG fueling depots. Excluding diesel vehicles, which need technology advances to use NG, savings of 8% are projected. Methanol use by large fleets could displace up to 8% of petroleum-based highway vehicle fuel from spark-ignition vehicles and another 9% from diesel vehicles with technology advances. The US NG supply appears adequate to accommodate fleet use. Supply projections, future price differential versus gasoline, and user economics are uncertain. In many cases, attractive paybacks can occur. Compressed NG now costs on average about $0.65 less than gasoline, per energy-equivalent gallon. Methanol supply projections, future prices, and user economics are even more uncertain. Current and projected near-term methanol supplies are far from adequate to support fleet use. Methanol presently costs more than gasoline on an equal-energy basis, but is projected to cost less if produced from coal instead of NG or petroleum.

  6. Sodium fast reactor: an asset for a PWR UOX/MOX fleet - 5327

    International Nuclear Information System (INIS)

    Tiphine, M.; Coquelet-Pascal, C.; Girieud, R.; Eschbach, R.; Chabert, C.; Grosman, R.

    2015-01-01

    Due to its low fissile content, Pu from spent MOX fuels is sometimes regarded as not recyclable in LWR. Based on the existing French nuclear infrastructure (La Hague reprocessing plant and MELOX MOX manufacturing plant), AREVA and CEA have evaluated the conditions of Pu multi recycling in a 100% LWR fleet. As France is currently supporting a Fast Reactor prototype project, scenario studies have also been conducted to evaluate the contribution of a 600 MWe SFR in the LWR fleet. These scenario studies consider a nuclear fleet composed of 8 PWR 900 MWe, with or without the contribution of a SFR, and aim at evaluating the following points: -) the feasibility of Pu multi-recycling in PWR; -) the impact on the spent fuels storage; -) the reduction of the stored separated Pu; -) the impact on waste management and final disposal. The studies have been conducted with the COSI6 code, developed by CEA Nuclear Energy Direction since 1985, that simulates the evolution over time of a nuclear power plants fleet and of its associated fuel cycle facilities and provides material flux and isotopic compositions at each point of the scenario. To multi-recycle Pu into LWR MOX and to ensure flexibility, different reprocessing strategies were evaluated by adjusting the reprocessing order, the choice of used fuel assemblies according to their burn-up and the UOX/MOX proportions. The improvement of the Pu fissile quality and the kinetic of Pu multi-recycling in SFR depending on the initial Pu quality were also evaluated and led to a reintroduction of Pu in PWR MOX after a single irradiation in SFR, still in dilution with Pu from UOX to maintain a sufficient fissile quality. (authors)

  7. Fleet Readiness Center - Southeast Technology Development Program (Cadmium & Hexavalent Chromium Reduction)

    Science.gov (United States)

    2014-11-01

    Fleet Readiness Center - Southeast TECHNOLOGY DEVELOPMENT PROGRAM (Cadmium & Hexavalent Chromium Reduction) Jack Benfer Senior Materials...Development Program (Cadmium & Hexavalent Chromium Reduction) 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT...Rinse Black Oxide Rinse CRES Passivation Chrome Plating Cadmium Plating Cadmium Brush Plating Class N (TRL 9) Class N (TRL 7) Class N (TRL 6

  8. Fuel conservation and GHG (Greenhouse gas) emissions mitigation scenarios for China’s passenger vehicle fleet

    International Nuclear Information System (INIS)

    Hao, Han; Wang, Hewu; Ouyang, Minggao

    2011-01-01

    Passenger vehicles are the main consumers of gasoline in China. We established a bottom-up model which focuses on the simulation of energy consumptions and greenhouse gas (GHG) emissions growth by China’s passenger vehicle fleet. The fuel conservation and GHG emissions mitigation effects of five measures including constraining vehicle registration, reducing vehicle travel, strengthening fuel consumption rate (FCR) limits, vehicle downsizing and promoting electric vehicle (EV) penetration were evaluated. Based on the combination of these measures, the fuel conservation and GHG emissions mitigation scenarios for China’s passenger vehicle fleet were analyzed. Under reference scenario with no measures implemented, the fuel consumptions and life cycle GHG emissions will reach 520 million tons of oil equivalent (Mtoe) and 2.15 billion tons in 2050, about 8.1 times the level in 2010. However, substantial fuel conservation can be achieved by implementing the measures. By implementing all five measures together, the fuel consumption will reach 138 Mtoe in 2030 and decrease to 126 Mtoe in 2050, which is only 37.1% and 24.3% of the consumption under reference scenario. Similar potential lies in GHG mitigation. The results and scenarios provided references for the Chinese government’s policy-making. -- Highlights: ► We established a bottom-up model to simulate the fuel consumptions and GHG (Greenhouse gas) emissions growth by China’s passenger vehicle fleet. ► Five measures including constraining vehicle registration, reducing vehicle travel, improving fuel efficiency, vehicle downsizing and promoting EV penetration were evaluated. ► The fuel conservation and GHG emissions mitigation scenarios for China’s passenger vehicle fleet were provided as references for policy-making.

  9. Wave theory analysis of the Hungarian vehi-cle fleet especially focusing on emission categories

    Directory of Open Access Journals (Sweden)

    Gabor SZENDRO

    2015-09-01

    Full Text Available Toyotarity means the improvement of quality, decrease of production costs and production time, increase of safety and morale. With the help of tools of toyotarity strategic factors can be determined, production or service problems can be revealed. In this paper tools of toyotarity are obtained to analyse the driving factors of Hungarian vehicle fleet and car renewal. Product analysis was conducted on the basis of wavetheory.

  10. A comparative analysis of performance and cost metrics associated with a diesel to biodiesel fleet transition

    International Nuclear Information System (INIS)

    Shrake, Scott O.; Landis, Amy E.; Bilec, Melissa M.; Collinge, William O.; Xue Xiaobo

    2010-01-01

    With energy security, economic stabilization, and environmental sustainability being at the forefront of US policy making, the development of biodiesel production and use within the United States has been growing at an astonishing rate. According to the latest DOE energy report, biodiesel production and consumption in the US has decreased since its peak in 2008, but still remains an important factor in the US energy mix. However, despite recent studies showing that B5 has similar performance qualities to that of the currently used ultra-low-sulfur petroleum diesel (ULSD) fleet managers and corporations still remain hesitant regarding a switch to B5. This research examined the major areas of concern that arise with transitioning fleets from ULSD to B5 with the goal of alleviating those concerns with quantitative results from an actual fleet implementation and transition. In conjunction with the Pennsylvania Department of Transportation (PennDOT) a comparison of cost, cold weather fuel properties, engine performance, fuel economy, and maintenance and repairs was conducted using data obtained over 3 years from a pilot study. The results found that B5 performed as well or better than ULSD in all performance metrics. - Research Highlights: →Conducted multi-year study of a DOT fleet transition from ULSD to biodiesel (B5)→No significant difference in cold weather performance, engine power output, or torque→No significant difference in fuel economy→No resulting increase in maintenance and repair costs or frequency→Life-cycle costing revealed no hidden costs as a result of B5 implementation

  11. Trends in the development and updating of the fishing fleet in the Northern Basin

    Directory of Open Access Journals (Sweden)

    Kuranov Yu. F.

    2017-12-01

    Full Text Available The practice and need to update and form the production and technical base of fishery enterprises have an increasing impact on performance of the fisheries complex and become decisive when considering the prospects for its development on an innovative basis. The paper examines the current state and development trends of the Northern Basin fishing fleet, its production capabilities in developing available bioresources, the need and prospects for renewal. During the research the influence of the main factors determining the formation of the quantitative and structural composition of the fishing fleet has been shown. The most stable and long-term impact is the state of commercial stocks of aquatic biological resources, their variability under the influence of natural factors and anthropogenic load. The second important factor is institutional changes. Initially, since the beginning of the 1990s, this took place due to the transformation of economic relations, and later – to institutional changes in the legislatively approved principles of granting fishing enterprises the right to access water biological resources. The following data have been presented: adaptation of the fleet's production capacities for specialization of fishing activities, ways of modernization and re-equipment, construction of new vessels, acquisition of vessels being in operation in the countries with developed fisheries. These changes had reduced the fleet's production potential raising its qualitative indicators (productivity, depth of processing of raw materials at sea. At the same time, the noted positive trends have been accompanied by some increase in the aging indicators for all groups and types of vessels. The institutional and economic instruments for supporting and stimulating the construction of new fishing vessels have been justified. Nowadays, in the Northern Basin the priority areas of support should be the construction of ships for pelagic fishing and coastal

  12. Tail swing performance of the South African car-carrier fleet

    CSIR Research Space (South Africa)

    De Saxe, C

    2012-09-01

    Full Text Available PERFORMANCE OF THE SOUTH AFRICAN CAR-CARRIER FLEET Obtained BSc and currently completing MSc at the University of the Witwatersrand. Researcher at the Council for Scientific and Industrial Research (CSIR) through the CSIR?s Studentship programme.... Obtained BSc and MSc from the University of the Witwatersrand and PhD from the University of Cambridge. Senior Lecturer in the School of Mechanical, Industrial and Aeronautical Engineering at the University of the Witwatersrand. C. DE SAXE...

  13. Real-driving emissions of circulating Spanish car fleet in 2015 using RSD Technology.

    Science.gov (United States)

    Pujadas, M; Domínguez-Sáez, A; De la Fuente, J

    2017-01-15

    In this paper we present the results corresponding to on-road traffic emissions measurements obtained during two field campaigns developed in the Madrid region (Spain) during 2014 and 2015 in the framework of the CORETRA project. The experimental strategy was based on the use of a RSD 4600 remote sensor in interurban roads. These measurements have produced a global database of >190,000 vehicles with their associated emission data (NO/CO 2 , HC/CO 2 and CO/CO 2 ), which can be considered representative of the current Spanish circulating fleet. The results of M1 vehicles were analysed according to their distribution by Euro Standard and engine model. One of the relevant findings is that, despite the progressive introduction of increasingly stringent standards, no NO emission reduction is observed for diesel vehicles with time, although this behavior shows significative differences among brands and engine models. We have also investigated the presence of "high emitter" (HE) vehicles in the Spanish M1 circulating fleet and most of the HE detected corresponded to diesel vehicles with very high NO/CO 2 values. With these results at hand, we strongly propose the future incorporation of the "high emitter vehicle" definition into the European environmental legislation, as well as the establishment of specific strategies in each country/region in order to identify these anomalous vehicles. Identification and repair of HE vehicles within the European circulating fleets, although are not easy tasks, should be considered very important for the improvement of air quality in the EU. The use of non-intrusive optical technologies (i.e. RSD) is an excellent option to provide instantaneous real emission data of each individual vehicle without disturbing traffic and for on-road fleet monitoring. In summary, it is a good strategy to obtain valuable information about the long term surveillance of real vehicle emission trends, specially after the introduction of new standard. Copyright

  14. Potential Cost Savings with 3D Printing Combined With 3D Imaging and CPLM for Fleet Maintenance and Revitalization

    Science.gov (United States)

    2014-05-01

    1 Potential Cost Savings with 3D Printing Combined With 3D Imaging and CPLM for Fleet Maintenance and Revitalization David N. Ford...2014 4. TITLE AND SUBTITLE Potential Cost Savings with 3D Printing Combined With 3D Imaging and CPLM for Fleet Maintenance and Revitalization 5a...Manufacturing ( 3D printing ) 2 Research Context Problem: Learning curve savings forecasted in SHIPMAIN maintenance initiative have not materialized

  15. AVTA federal fleet PEV readiness data logging and characterization study for the National Park Service: Grand Canyon National Park

    Energy Technology Data Exchange (ETDEWEB)

    Schey, Stephen [Intertek Testing Services, Phoenix, AZ (United States); Francfort, Jim [Idaho National Lab. (INL), Idaho Falls, ID (United States); Nienhueser, Ian [Intertek Testing Services, Phoenix, AZ (United States)

    2014-08-01

    This report focuses on the Grand Canyon National Park (GCNP) fleet to identify daily operational characteristics of select vehicles and report findings on vehicle and mission characterizations to support the successful introduction of PEVs into the agencies’ fleets. Individual observations of these selected vehicles provide the basis for recommendations related to electric vehicle adoption and whether a battery electric vehicle or plug-in hybrid electric vehicle (collectively PEVs) can fulfill the mission requirements.

  16. Zinc injection on the EDF fleet monitoring the injection on 12 units

    International Nuclear Information System (INIS)

    Le-Meur, Gaelle Harmand; Anne-Marie; Stutzmann, Agnes; Taunier, Stephane; Benfarah, Moez; Bretelle, Jean-Luc; Alain, Rocher; Claeys, Myriam; Bonne, Sebastien

    2012-09-01

    After a first implementation of zinc injection at Bugey 2 and Bugey 4, EDF decided to extend the program to other units of its fleet. 14 more reactors from the French fleet of 58 were chosen in order to - Reduce the radiation sources for curative or preventive (after SGR) reasons - Mitigate stress corrosion cracking on nickel alloys and reduce the rate of generalized corrosion - Prevent the risk of CIPS, mainly after a fuel management change. Zinc injection started on 9 new units in 2011, 1 unit in 2012 and will be extended to 4 other units before the end of 2013. To monitor the injection, EDF has defined a complete program concerning chemistry, radiation protection (dose rate and deposited activities measurements), materials (statistical analysis of SG tube cracks), fuel (oxide measurements) and waste (radiochemical characterization of filters). Reference units were chosen for each field because of the size of the fleet. This paper will detail the different monitoring programs on the EDF plants injecting zinc. (authors)

  17. Transport fleet sizing by using make and buy decision-making

    Directory of Open Access Journals (Sweden)

    Stojanović Đurđica

    2011-01-01

    Full Text Available Cost reduction is among the main quoted reasons for logistics outsourcing, while transport capacities and operations are among the most outsourced logistics areas. However, according to transaction costs theory, there is often room for transport insourcing. Furthermore, nowadays many authors stress that mixed solutions can give better results than “make” or “buy” alternatives. “Make or buy” decision-making normative models, methods, and procedures in transport planning are not much explored. Instead, research is rather focused on carrier selection techniques, whereby it is supposed that outsourcing transport capacities is the most suitable solution. The purpose of this paper is to contribute to the body of literature on classical “makeor-buy” decision-making in transport fleet sizing in a non-logistics enterprise. It goes beyond the basic “make or buy” decisionmaking, and intermediate solutions (i.e., “make and buy” are explored. Practical directions are given according to theoretical principles, and a case study is used to exemplify the main deductions. The research also has practical implications. Many enterprises in transition economies have faced the question of what to do with their in-house transport fleets. The obtained results are of interest to merchants and manufacturing enterprises that already have a private transport fleet and are considering how to rationalize it.

  18. Multi-objective optimization for nuclear fleet evolution scenarios using COSI - 5187

    International Nuclear Information System (INIS)

    Freynet, D.; Coquelet-Pascal, C.; Eschbach, R.; Krivtchik, G.; Merle-Lucotte, E.

    2015-01-01

    The consequences of strategic choices on material inventories and flux in the fuel cycle can be analysed with the COSI code. Indeed COSI enables to compare various fleet evolution options (e.g. new reactor systems deployment) and different nuclear materials management (e.g. plutonium multi-recycling). COSI is coupled with the CESAR depletion code. In this paper a methodology for the nuclear fleet evolution scenarios optimization using COSI is introduced. A large number of scenario calculations is needed to solve an optimization problem, which makes infeasible an optimization calculation using the COSI/CESAR version. Given that CESAR calculations represent about 95% of the COSI computation time, CESAR irradiation surrogate models carrying out with ANN regression method and cooling analytic models have been coupled with COSI. An example of optimization study is presented involving 2 discrete variables related to the number of deployed SFR to renew the French PWR fleet and 2 criteria: minimizing the natural uranium consumption and the number of produced HLW vitrified packages

  19. Refueling Behavior of Flexible Fuel Vehicle Drivers in the Federal Fleet

    Energy Technology Data Exchange (ETDEWEB)

    Daley, R.; Nangle, J.; Boeckman, G.; Miller, M.

    2014-05-01

    Federal fleets are a frequent subject of legislative and executive efforts to lead a national transition to alternative fuels and advanced vehicle technologies. Section 701 of the Energy Policy Act of 2005 requires that all dual-fueled alternative fuel vehicles in the federal fleet be operated on alternative fuel 100% of the time when they have access to it. However, in Fiscal Year (FY) 2012, drivers of federal flex fuel vehicles (FFV) leased through the General Services Administration refueled with E85 24% of the time when it was available--falling well short of the mandate. The U.S. Department of Energy's National Renewable Energy Laboratory completed a 2-year Laboratory Directed Research and Development project to identify the factors that influence the refueling behavior of federal FFV drivers. The project began with two primary hypotheses. First, information scarcity increases the tendency to miss opportunities to purchase E85. Second, even with perfect information, there are limits to how far drivers will go out of their way to purchase E85. This paper discusses the results of the project, which included a June 2012 survey of federal fleet drivers and an empirical analysis of actual refueling behavior from FY 2009 to 2012. This research will aid in the design and implementation of intervention programs aimed at increasing alternative fuel use and reducing petroleum consumption.

  20. Challenges and Approaches for Developing Ultrafine Particle Emission Inventories for Motor Vehicle and Bus Fleets

    Directory of Open Access Journals (Sweden)

    Diane U. Keogh

    2011-03-01

    Full Text Available Motor vehicles in urban areas are the main source of ultrafine particles (diameters < 0.1 µm. Ultrafine particles are generally measured in terms of particle number because they have little mass and are prolific in terms of their numbers. These sized particles are of particular interest because of their ability to enter deep into the human respiratory system and contribute to negative health effects. Currently ultrafine particles are neither regularly monitored nor regulated by ambient air quality standards. Motor vehicle and bus fleet inventories, epidemiological studies and studies of the chemical composition of ultrafine particles are urgently needed to inform scientific debate and guide development of air quality standards and regulation to control this important pollution source. This article discusses some of the many challenges associated with modelling and quantifying ultrafine particle concentrations and emission rates for developing inventories and microscale modelling of motor vehicles and buses, including the challenge of understanding and quantifying secondary particle formation. Recommendations are made concerning the application of particle emission factors in developing ultrafine particle inventories for motor vehicle fleets. The article presents a précis of the first published inventory of ultrafine particles (particle number developed for the urban South-East Queensland motor vehicle and bus fleet in Australia, and comments on the applicability of the comprehensive set of average particle emission factors used in this inventory, for developing ultrafine particle (particle number and particle mass inventories in other developed countries.

  1. Modelling energy demand for a fleet of hydrogen-electric vehicles interacting with a clean energy hub

    International Nuclear Information System (INIS)

    Syed, F.; Fowler, M.; Wan, D.; Maniyali, Y.

    2009-01-01

    This paper details the development of an energy demand model for a hydrogen-electric vehicle fleet and the modelling of the fleet interactions with a clean energy hub. The approach taken is to model the architecture and daily operation of every individual vehicle in the fleet. A generic architecture was developed based on understanding gained from existing detailed models used in vehicle powertrain design, with daily operation divided into two periods: charging and travelling. During the charging period, the vehicle charges its Electricity Storage System (ESS) and refills its Hydrogen Storage System (HSS), and during the travelling period, the vehicle depletes the ESS and HSS based on distance travelled. Daily travel distance is generated by a stochastic model and is considered an input to the fleet model. The modelling of a clean energy hub is also presented. The clean energy hub functions as an interface between electricity supply and the energy demand (i.e. hydrogen and electricity) of the vehicle fleet. Finally, a sample case is presented to demonstrate the use of the fleet model and its implications on clean energy hub sizing. (author)

  2. Opposite effect of photocorrosion on photocatalytic performance among various AgxMyOz/TiO2 (M = C, P) photocatalysts: A novel effective method for preparing Ag/TiO2 composite

    Science.gov (United States)

    Feng, Caixia; Pang, Yuhua; Wang, Yan; Sun, Mingming; Zhang, Chenyan; Zhang, Ling; Zhou, Yanmei; Li, Deliang

    2016-07-01

    Three kinds of hybrids, Ag2CO3/TiO2, Ag2C2O4/TiO2 and Ag3PO4/TiO2 comprising of P25-TiO2 and silver-containing photocatalyst, (together coded as AgxMyOz/TiO2 (M = C, P)) were prepared via a facile precipitation method. The photocatalytic activity and stability of the as-prepared AgxMyOz/TiO2 was compared by monitoring the oxidation of propylene under visible light irradiation. Results showed that both Ag2CO3/TiO2 and Ag2C2O4/TiO2 exhibit perfect performance with a high propylene degradation removal rate of 88% and 78%, respectively, during four successive experimental runs. On the contrary, for Ag3PO4/TiO2, the photocatalytic activity gradually declines to 8% from 32% under the same conditions. In order to explore the reason for the above remarkable difference in activity and stability over AgxMyOz/TiO2, X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and UV-vis diffuse reflectance spectroscopy (DRS) were used to investigate the change of AgxMyOz/TiO2 before and after irradiation. It was found that three silver-containings, Ag2CO3, Ag2C2O4 and Ag3PO4 on the surface of TiO2, all experienced photo-corrosion to various extents during irradiation process. Surprisingly, the effect of photo-corrosion on visible light activity and stability among various AgxMyOz/TiO2 is very different. For both Ag2CO3 and Ag2C2O4, they are easily decomposed into metallic Ag and CO2, and gaseous CO2 escaped from catalyst leaving silver nanoparticles on the surface of TiO2 resulted in the formation of plasmonic photocatalyst Ag/TiO2. The synergetic effect between surface plasma resonance of silver and interfacial electron transfer over the obtained Ag/TiO2 heterojunctions is in favor of the superior photocatalytic performance under visible light. While for Ag3PO4/TiO2, Ag3PO4 on the surface of TiO2 is partially photo-decomposed into Ag and phosphorus oxide and the phosphorus oxide covering on the surface of undecomposed Ag3PO4/TiO2 deactivates its photocatalytic performance

  3. Experimental autoimmune encephalomyelitis: Association with mutual regulation of RelA (p65)/NF-κB and phospho-IκB in the CNS

    International Nuclear Information System (INIS)

    Hwang, Insun; Ha, Danbee; Ahn, Ginnae; Park, Eunjin; Joo, Haejin; Jee, Youngheun

    2011-01-01

    Highlights: → The phosphorylation of RelA's inhibitory factor IκB and subsequent RelA activation are important to the disease process of EAE. → The expression of RelA and phospho-IκB was markedly increased in the initiation and during the progression of EAE. → TPCK-treated EAE mice showed lower incidence of EAE with less severe symptoms and quicker recovery than vehicle-treated EAE mice. → TPCK significantly suppressed the MOG 35-55 -specific T cell proliferation by reducing the production of IFN-γ and IL-17 cytokines in EAE. → The NF-κB cascade's activity increased gradually with the development of symptoms and brain pathology of EAE. -- Abstract: Recently emerging evidence that the NF-κB family plays an important role in autoimmune disease has produced very broad and sometimes paradoxical conclusions. In the present study, we elucidated that the activation of RelA (p65) of NF-κB and IκB dissociation assumes a distinct role in experimental autoimmune encephalomyelitis (EAE) progression by altering IκB phosphorylation and/or degradation. In the present study of factors that govern EAE, the presence and immunoreactivity of nuclear RelA and phospho-IκB were recorded at the initiation and peak stage, and degradation of IκBα progressed rapidly at an early stage then stabilized during recovery. The immunoreactivity to RelA and phospho-IκB occurred mainly in inflammatory cells and microglial cells but only slightly in astrocytes. Subsequently, the blockade of IκB dissociation from NF-κB reduced the severity of disease by decreasing antigen-specific T cell response and production of IL-17 in EAE. Thus, blocking the dissociation of IκB from NF-κB can be utilized as a strategy to inhibit the NF-κB signal pathway thereby to reduce the initiation, progression, and severity of EAE.

  4. Antimalarial activity of artefenomel (OZ439), a novel synthetic antimalarial endoperoxide, in patients with Plasmodium falciparum and Plasmodium vivax malaria: an open-label phase 2 trial.

    Science.gov (United States)

    Phyo, Aung Pyae; Jittamala, Podjanee; Nosten, François H; Pukrittayakamee, Sasithon; Imwong, Mallika; White, Nicholas J; Duparc, Stephan; Macintyre, Fiona; Baker, Mark; Möhrle, Jörg J

    2016-01-01

    Artefenomel (OZ439) is a novel synthetic trioxolane with improved pharmacokinetic properties compared with other antimalarial drugs with the artemisinin pharmacophore. Artefenomel has been generally well tolerated in volunteers at doses up to 1600 mg and is being developed as a partner drug in an antimalarial combination treatment. We investigated the efficacy, tolerability, and pharmacokinetics of artefenomel at different doses in patients with Plasmodium falciparum or Plasmodium vivax malaria. This phase 2a exploratory, open-label trial was done at the Hospital for Tropical Diseases, Bangkok, and the Shoklo Malaria Research Unit in Thailand. Adult patients with acute, uncomplicated P falciparum or P vivax malaria received artefenomel in a single oral dose (200 mg, 400 mg, 800 mg, or 1200 mg). The first cohort received 800 mg. Testing of a new dose of artefenomel in a patient cohort was decided on after safety and efficacy assessment of the preceding cohort. The primary endpoint was the natural log parasite reduction per 24 h. Definitive oral treatment was given at 36 h. This trial is registered with ClinicalTrials.gov, number NCT01213966. Between Oct 24, 2010, and May 25, 2012, 82 patients were enrolled (20 in each of the 200 mg, 400 mg, and 800 mg cohorts, and 21 in the 1200 mg cohort). One patient withdrew consent (before the administration of artefenomel) but there were no further dropouts. The parasite reduction rates per 24 h ranged from 0·90 to 1·88 for P falciparum, and 2·09 to 2·53 for P vivax. All doses were equally effective in both P falciparum and P vivax malaria, with median parasite clearance half-lives of 4·1 h (range 1·3-6·7) to 5·6 h (2·0-8·5) for P falciparum and 2·3 h (1·2-3·9) to 3·2 h (0·9-15·0) for P vivax. Maximum plasma concentrations, dose-proportional to 800 mg, occurred at 4 h (median). The estimated elimination half-life was 46-62 h. No serious drug-related adverse effects were reported; other adverse effects were

  5. Multi-Year On-Road Emission Factor Trends of Two Heavy-Duty California Fleets

    Science.gov (United States)

    Haugen, M.; Bishop, G.

    2017-12-01

    New heavy-duty vehicle emission regulations have resulted in the development of advanced exhaust after-treatment systems that specifically target particulate matter (PM) and nitrogen oxides (NOx = NO + NO2). This has resulted in significant decreases in the emissions of these species. The University of Denver has collected three data sets of on-road gaseous (CO, HC, NO and NOx) and PM (particle mass, black carbon and particle number) emission measurements from heavy-duty vehicles (HDVs) in the spring of 2013, 2015 and 2017 at two different locations in California. One site is located at the Port of Los Angeles, CA (1,150 HDVs measured in 2017) and the other site is located at a weigh station in Northern California near Cottonwood, CA (780 HDVs measured in 2017). The On-Road Heavy-Duty Measurement Setup measures individual HDV's fuel specific emissions (DOI: 10.1021/acs.est.6b06172). Vehicles drive under a tent-like structure that encapsulates vehicle exhaust and 15 seconds of data collection is integrated to give fuel specific information. The measurements obtained from these campaigns contain real-world emissions affected by different driving modes, after-treatment systems and location. The Port of Los Angeles contributes a fleet that is fully equipped with diesel particulate filters (DPFs) as a result of the San Pedro Ports Clean Air Action Plan enforced since 2010 that allows only vehicles model year 2007 or newer on the premises. This fleet, although comprised with relatively new HDVs with lower PM emissions, has increased PM emissions as it has aged. Cottonwood's fleet contains vehicles with and without after-treatment systems, a result of a gradual turnover rate, and fleet PM has decreased at a slower rate than at the Port of Los Angeles. The decrease in PM emissions is a result of more HDVs being newer model years as well as older model years being retrofit with DPFs. The complimentary fleets, studied over multiple years, have given the University of Denver

  6. Step on the gas : Calgary-based firm increases efficiency of compressor fleets

    Energy Technology Data Exchange (ETDEWEB)

    Cope, G.

    2005-11-01

    Many operators are reluctant to run their compressors close to optimum efficiency due to concerns that a frozen well or other problems will push units over their limit, and gas limited areas also cause the machines to be run at partial capacity. This article suggested that the key to compressor optimization lies in the correct identification of deficiencies. Petroleum companies gather thousands of compressor data points every day, ranging from temperature and pressure to control valve position and compressor volume flow, but there is no easy way of analyzing the information. A software prototype called Production Equipment Performance Reporting (PEPR) is capable of disseminating log data and model compressor performance, as well as reporting results for an entire fleet. Raw operating data is gathered for the entire fleet and compressor utilization is ranked to show which compressors are being used efficiently and which ones are lagging. Data is processed from selected points per unit and then divided into key performance indicators (KPI), such as power utilization. To experienced operators, KPIs offer concise information and a range of changes they can make to improve the efficiency of their fleets. It was noted that Devon ran PEPR at one of its major gas fields, and was so satisfied with the results that it has now applied the system to the company's entire fleet. PEPR Inc. was formed in August 2004 to commercially market the production optimization software. In addition to optimizing compressor utilization, the software also handles other important functions, such as asset management, as well as generating downtime reports. While it only takes a day to install the software and a day to train, documenting basic data for each compressor can take several months for a large fleet. It was concluded that PEPR plans to configure its software to other production-related equipment. The system is capable of using operating parameters to calculate emissions instead of

  7. Instruments to reduce pollutant emissions of the existing inland vessel fleet. Position paper for international workshop 'Emissions from the Legacy Fleet'

    Energy Technology Data Exchange (ETDEWEB)

    Den Boer, E.

    2011-11-15

    Inland waterway transport (IWT) has a key performance on the GHG emissions per tonne kilometre shipped. Due to its potential to limit climate change, the recent EU Transport White Paper has set high goals for the non-road modes. The Ports of Rotterdam (NL), Antwerp (BE) and others strive to increase the use of IWT in their hinterland transport. The Port of Rotterdam authority has imposed a modal split on the newly built container terminals, thus increasing the use of rail and IWT. The growth is estimated to result in a quadrupling of inland barge container traffic on the Rhine corridor in the timeframe 2010-2035. Local air quality is another environmental issue, however, that plays a key role. Due to reasons of long ship engine lifetimes and progress made in road transport emissions, IWT needs to improve its air pollution profile. To turn the potential of IWT into real growth, it is important to: improve the air pollutant profile of inland shipping; take responsibility to maintain the air quality levels along inland waterway corridors over Europe, especially in urban areas where road transport, industry and IWT contribute to levels that will need to be in accordance with the EU air quality directive 2008/51. A new set of standards for new engines will shortly be proposed by the European Commission to be introduced in 2016. However, these will probably not be as tight as the Euro-VI standards for road transport. In addition, the long lifetime of inland barge engines (30,000 to over 200,000 hours, depending on the engine type) will result in a slow uptake of the phase-IV engines in the fleet. The German and Dutch authorities have the opinion that not only the air pollutant emissions of new engines need to be curbed, but deliberate over the development of instruments that will reduce the pollutant emissions of the existing fleet (legacy fleet), in addition to the limitedly effective subsidy schemes applied in recent years. This paper demonstrates the need for measures

  8. Assessment of early changes in 3H-fluorothymidine uptake after treatment with gefitinib in human tumor xenograft in comparison with Ki-67 and phospho-EGFR expression

    International Nuclear Information System (INIS)

    Zhao, Songji; Kuge, Yuji; Zhao, Yan; Takeuchi, Satoshi; Hirata, Kenji; Takei, Toshiki; Shiga, Tohru; Dosaka-Akita, Hirotoshi; Tamaki, Nagara

    2013-01-01

    The purpose of this study was to evaluate whether early changes in 3′-deoxy-3′- 3 H-fluorothymidine ( 3 H-FLT) uptake can reflect the antiproliferative effect of gefitinib in a human tumor xenograft, in comparison with the histopathological markers, Ki-67 and phosphorylated EGFR (phospho-EGFR). An EGFR-dependent human tumor xenograft model (A431) was established in female BALB/c athymic mice, which were divided into three groups: one control group and two treatment groups. Mice in the treatment groups were orally administered a partial regression dose (100 mg/kg/day) or the maximum tolerated dose of gefitinib (200 mg/kg/day), once daily for 2 days. Mice in the control group were administered the vehicle (0.1% Tween 80). Tumor size was measured before and 3 days after the start of treatment. Biodistribution of 3 H-FLT and 18 F-FDG (%ID/g/kg) was examined 3 days after the start of the treatment. Tumor cell proliferative activity with Ki-67 was determined. Immunohistochemical staining of EGFR and measurement of phospho-EGFR were also performed. High expression levels of EGFR and Ki-67 were observed in the A431 tumor. After the treatment with 100 and 200 mg/kg gefitinib, the uptake levels of 3 H-FLT in the tumor were significantly reduced to 67% and 61% of the control value, respectively (0.39 ± 0.09, 0.36 ± 0.06, 0.59 ± 0.11%ID/g/kg for 100 mg/kg, 200 mg/kg, and control groups, respectively; p < 0.01 vs. control), but those of 18 F-FDG were not. After the treatment with 100 and 200 mg/kg gefitinib, the expression levels of Ki-67 in the tumor were markedly decreased (4.6 ± 2.4%, 6.2 ± 1.8%, and 10.4 ± 5.7% for 100 mg/kg, 200 mg/kg, and control groups, respectively, p < 0.01 vs. control). The expression levels of the phospho-EGFR protein also significantly decreased (29% and 21% of the control value for 100, and 200 mg/kg, respectively p < 0.01 vs. control). There was no statistically significant difference in tumor size between pre- and post-treatments in

  9. A phospho-sugar binding domain homologous to NagB enzymes regulates the activity of the central glycolytic genes repressor.

    Science.gov (United States)

    Doan, Thierry; Martin, Laetitia; Zorrilla, Silvia; Chaix, Denis; Aymerich, Stéphane; Labesse, Gilles; Declerck, Nathalie

    2008-06-01

    CggR belongs to the SorC family of bacterial transcriptional regulators which control the expression of genes and operons involved in carbohydrate catabolism. CggR was first identified in Bacillus subtilis where it represses the gapA operon encoding the five enzymes that catalyze the central part of glycolysis. Here we present a structure/function study demonstrating that the C-terminal region of CggR regulates the DNA binding activity of this repressor in response to binding of a phosphorylated sugar. Molecular modeling of CggR revealed a winged-helix DNA-binding motif followed by a C-terminal domain presenting weak but significant homology with glucosamine-6-phosphate deaminases from the NagB family. In silico ligand screening suggested that the CggR C-terminal domain would bind preferentially bi-phosphorylated compounds, in agreement with previous studies that proposed fructuose-1,6-biphosphate (FBP) as the inducer metabolite. In vitro, FBP was the only sugar compound capable of interfering with CggR cooperative binding to DNA. FBP was also found to protect CggR against trypsin degradation at two arginine residues predicted to reside in a mobile loop forming the active site lid of the NagB enzymes. Replacement of residues predicted to interact with FBP led to mutant CggR with altered repressor activity in vivo but retaining their structural integrity and DNA binding activity in vitro. Interestingly, some of the mutant repressors responded with different specificity towards mono- and di-phospho-fructosides. Based on these results, we propose that the activity of the CggR-like repressors is controlled by a phospho-sugar binding (PSB) domain presenting structural and functional homology with NagB enzymes. (c) 2008 Wiley-Liss, Inc.

  10. Comparison of HER2 and phospho-HER2 expression between biopsy and resected breast cancer specimens using a quantitative assessment method.

    Directory of Open Access Journals (Sweden)

    Yalai Bai

    Full Text Available BACKGROUND: HER2/Neu (ErbB-2 overexpression, which occurs in 15-20% of breast cancer cases, is associated with better response to treatment with the drug trastuzumab. PhosphoHER2 (pHER2 has been evaluated for prediction of response to trastuzumab. Both markers are heterogeneously detected and are potentially subject to loss as a consequence of delayed time to fixation. Here, we quantitatively assess both markers in core needle biopsies (CNBs and matched tumor resections to assess concordance between the core and the resection and between HER2 and pHER2. METHODS: A selected retrospective collection of archival breast cancer cases yielded 67 cases with both core and resection specimens. Both HER2 and pTyr(1248HER2 were analyzed by the AQUA® method of quantitative immunofluorescence on each specimen pair. RESULTS: Both HER2 immunoreactivity (P<0.0001 and pTyr(1248HER2 immunoreactivity (P<0.0001 were lower in resections relative to CNB specimens. However, clinical implications of this change may not be evident since no case changed from 3+ (CNB to negative (resection. Assessment of pTyr(1248HER2 showed no direct correlation with HER2 in either CNB or resection specimens. CONCLUSIONS: The data suggest that measurement of both HER2 and phospho- Tyr(1248HER2, in formalin-fixed tissue by immunological methods is significantly affected by pre-analytic variables. The current study warrants the adequate handling of resected specimens for the reproducible evaluation of HER2 and pHER2. The level of pTyr(1248HER2, was not correlated to total HER2 protein. Further studies are required to determine the significance of these observations with respect to response to HER2 directed therapies.

  11. Assessment of pathways to reduce CO2 emissions from passenger car fleets: Case study in Ireland

    International Nuclear Information System (INIS)

    Alam, Md. Saniul; Hyde, Bernard; Duffy, Paul; McNabola, Aonghus

    2017-01-01

    Highlights: • Integration of models provides a robust estimation of tailpipe CO 2 emissions. • Taxation impact of vehicle fleet dieselisation was modelled. • A scenario development approach was proposed for policy analysis. • EV provided the largest cost saving option than that of the other fuel technologies. - Abstract: This study modelled the Passenger (PC) fleet and other categories of road transport in Ireland from 2015 to 2035 to assess the impact of current and potential greenhouse gas mitigation policies on CO 2 emissions. Scenarios included the shift of purchasing towards diesel PCs over gasoline PCs. Scrappage rates were also calculated and applied to the fleet to predict future sales of PCs. Seven future policy scenarios were examined using different penetrations of PC sales for different vehicle technologies under current and alternative bio-fuel obligations. Tank to Wheel (T2W) tailpipe and Well to Wheel (W2W) CO 2 emissions, and energy demand were modelled using COPERT 4v11.3 and a recently published W2W CO 2 emissions model. A percentage reduction of conventional diesel and petrol vehicles, in different scenarios compared to a baseline scenario in the W2W model was applied to estimate the likely changes in T2W emissions at the tailpipe up to 2035. The results revealed that the biofuel policy scenario was insufficient in achieving a significant reduction of CO 2 emissions. However, without a fixed reduction target for CO 2 from the road transport sector, the success of policy scenarios in the long run is difficult to compare. The current Electric vehicle (EV) policy in Ireland is required to be implemented to reduce CO 2 emissions by a significant level. Results also show that a similar achievement of CO 2 emission reduction could be possible by using alternative vehicle technologies with higher abatement cost. However, as EV based policies have not been successful so far, Ireland may need to search for alternative pathways.

  12. 2005 nonroad engine fleet characterization in the Canadian Lower Fraser Valley : final report

    International Nuclear Information System (INIS)

    Mak, J.; Chan, N.; Campbell, K.; Preston, K.; Bolechowsky, K.

    2007-12-01

    Metro Vancouver conducts an emission inventory for the Lower Fraser Valley on a five year basis. This report presented an estimate of the nonroad engine fleet population and emissions in the Canadian portion of the Lower Fraser Valley (CLFV). The nonroad engine fleet includes internal combustion engines of different fuel types used in mobile equipment such as on-road vehicles, aircraft, locomotives and ocean-going marine vessels. Some examples of nonroad equipment that were estimated included agricultural tractors; airport ground equipment; forklifts; excavators; generator sets; lawn mowers; railroad maintenance equipment; pleasure boats; and off-road motorcycles. The purpose of the study was to assist Metro Vancouver and other levels of government in determining what progress has been made in improving air quality, as well as the effect of policies and regulations on the environment in terms of nonroad vehicles. The report presented the objectives of the nonroad engine fleet characterization project which were to review the current data on nonroad engine populations and associated information, and confirm or improve the data through appropriate means; prepare estimates of 2005 emissions in the CLFV based on the revised engine counts using the United States Environmental Protection Agency's nonroad 2005 model; and prepare backcasts and forecasts of the 2005 nonroad engine emission estimates for 1990 to 2030 in five-year increments. Results were presented and analysed into the following 9 equipment type categories: agricultural, airport ground support, commercial, construction, industrial, lawn and garden, railway maintenance, recreational marine and recreational off-road vehicle. Four fuel types were considered for each type of equipment, notably gasoline, diesel, liquefied petroleum gases and compressed natural gas. The report described the methodologies and sources and presented the equipment population data. Emission results for carbon monoxide, nitrogen oxides

  13. The design and implementation of a garbage truck fleet management system

    Directory of Open Access Journals (Sweden)

    Chen, C. H.

    2016-05-01

    Full Text Available In recent years, the improvement of cloud computing and mobile computing techniques has led to the availability of a variety of mobile applications (‘apps’ in the app store. For instance, a garbage truck app that can provide the immediate location of a garbage truck, the location of collection points, and forecasted arrival times of garbage trucks would be useful for mobile users. Since the power consumption of apps on mobile devices if of concern to mobile users, an optimised power-saving mechanism for updating messages, which is based on location information, for a proposed garbage truck fleet management system (GTFMS is proposed and implemented in this paper. The GTFMS is a three- component system that includes the on-board units on garbage trucks, a fleet management system, and a garbage truck app. In this study, an arrival time forecasting method is designed and implemented in the fleet management system, so that the garbage truck app can retrieve the forecasted arrival time via web services. A message updating event is then triggered that reports the location of garbage truck and the forecasted arrival time. In experiments conducted on case studies, the results showed that the mean accuracy of predicted arrival time by the proposed method is about 81.45 per cent. As for power consumption, the cost of traditional mobile apps is 2,880 times that of the mechanism proposed in this study. Consequently, the GTFMS can provide the precise forecasted arrival time of garbage trucks to mobile users, while consuming less power.

  14. Mapping sub-surface geostrophic currents from altimetry and a fleet of gliders

    Science.gov (United States)

    Alvarez, A.; Chiggiato, J.; Schroeder, K.

    2013-04-01

    Integrating the observations gathered by different platforms into a unique physical picture of the environment is a fundamental aspect of networked ocean observing systems. These are constituted by a spatially distributed set of sensors and platforms that simultaneously monitor a given ocean region. Remote sensing from satellites is an integral part of present ocean observing systems. Due to their autonomy, mobility and controllability, underwater gliders are envisioned to play a significant role in the development of networked ocean observatories. Exploiting synergism between remote sensing and underwater gliders is expected to result on a better characterization of the marine environment than using these observational sources individually. This study investigates a methodology to estimate the three dimensional distribution of geostrophic currents resulting from merging satellite altimetry and in situ samples gathered by a fleet of Slocum gliders. Specifically, the approach computes the volumetric or three dimensional distribution of absolute dynamic height (ADH) that minimizes the total energy of the system while being close to in situ observations and matching the absolute dynamic topography (ADT) observed from satellite at the sea surface. A three dimensional finite element technique is employed to solve the minimization problem. The methodology is validated making use of the dataset collected during the field experiment called Rapid Environmental Picture-2010 (REP-10) carried out by the NATO Undersea Research Center-NURC during August 2010. A marine region off-shore La Spezia (northwest coast of Italy) was sampled by a fleet of three coastal Slocum gliders. Results indicate that the geostrophic current field estimated from gliders and altimetry significantly improves the estimates obtained using only the data gathered by the glider fleet.

  15. On the road to fossil-free public transport: The case of Swedish bus fleets

    International Nuclear Information System (INIS)

    Xylia, Maria; Silveira, Semida

    2017-01-01

    Public transport is important for Sweden to acquire a fossil-fuel independent vehicle fleet by 2030. The aim of this paper is to assess the status of Swedish public bus fleets towards decarbonization, and explore factors affecting regional performance variations and fuel choices. Environmental performance indicators such as renewable fuel shares, CO 2 emissions, and energy efficiency are analyzed nationally and regionally. Fuel preferences and best practices are investigated through a survey and interviews with experts working with strategic planning at Public Transport Authorities. Almost 60% of the bus transport volume ran on renewables in 2014 compared to 8% in 2007, but regional variations are significant, partly due to factors such as driving conditions, bus and fuel types, typical trip lengths, and climatic conditions. However, there is no strong correlation between population densities or bus transport volume and the share of renewables achieved. This places political will, strategic planning and policies to promote public transport as key factors affecting renewable fuel deployment. Environmental factors are a priority when choosing fuels, while barriers to renewable fuels are mainly economic and political. Meanwhile, despite the overall progress, achievements in energy efficiency improvement are falling short in comparison to emissions reduction and adoption of renewable fuels, thus requiring further attention. - Highlights: • The penetration of renewables in Swedish public bus fleets reached ca. 60% in 2014. • Public bus emissions per vehicle-km have decreased by 43% between 2007 and 2014. • Efforts are needed to improve energy efficiency in bus transport. • Electricity is likely to receive increased attention according to expert survey.

  16. Assessing the Future Vehicle Fleet Electrification: The Impacts on Regional and Urban Air Quality.

    Science.gov (United States)

    Ke, Wenwei; Zhang, Shaojun; Wu, Ye; Zhao, Bin; Wang, Shuxiao; Hao, Jiming

    2017-01-17

    There have been significant advancements in electric vehicles (EVs) in recent years. However, the different changing patterns in emissions at upstream and on-road stages and complex atmospheric chemistry of pollutants lead to uncertainty in the air quality benefits from fleet electrification. This study considers the Yangtze River Delta (YRD) region in China to investigate whether EVs can improve future air quality. The Community Multiscale Air Quality model enhanced by the two-dimensional volatility basis set module is applied to simulate the temporally, spatially, and chemically resolved changes in PM 2.5 concentrations and the changes of other pollutants from fleet electrification. A probable scenario (Scenario EV1) with 20% of private light-duty passenger vehicles and 80% of commercial passenger vehicles (e.g., taxis and buses) electrified can reduce average PM 2.5 concentrations by 0.4 to 1.1 μg m -3 during four representative months for all urban areas of YRD in 2030. The seasonal distinctions of the air quality impacts with respect to concentration reductions in key aerosol components are also identified. For example, the PM 2.5 reduction in January is mainly attributed to the nitrate reduction, whereas the secondary organic aerosol reduction is another essential contributor in August. EVs can also effectively assist in mitigating NO 2 concentrations, which would gain greater reductions for traffic-dense urban areas (e.g., Shanghai). This paper reveals that the fleet electrification in the YRD region could generally play a positive role in improving regional and urban air quality.

  17. Baseline knowledge on vehicle safety and head restraints among Fleet Managers in British Columbia Canada: a pilot study.

    Science.gov (United States)

    Desapriya, Ediriweera; Hewapathirane, D Sesath; Peiris, Dinithi; Romilly, Doug; White, Marc

    2011-09-01

    Whiplash is the most common injury type arising from motor vehicle collisions, often leading to long-term suffering and disability. Prevention of such injuries is possible through the use of appropriate, correctly positioned, vehicular head restraints. To survey the awareness and knowledge level of vehicle fleet managers in the province of British Columbia, Canada, on the topics of vehicle safety, whiplash injury, and prevention; and to better understand whether these factors influence vehicle purchase/lease decisions. A survey was administered to municipal vehicle fleet managers at a professional meeting (n = 27). Although many respondents understood the effectiveness of vehicle head restraints in the prevention of whiplash injury, the majority rarely adjusted their own headrests. Fleet managers lacked knowledge about the seriousness of whiplash injuries, their associated costs for Canada's healthcare system, and appropriate head restraint positions to mitigate such injuries. The majority of respondents indicated that fleet vehicle purchase/lease decisions within their organization did not factor whiplash prevention as an explicit safety priority. There is relatively little awareness and enforcement of whiplash prevention strategies among municipal vehicle fleet managers.

  18. Japanese and Taiwanese pelagic longline fleet dynamics and the impacts of climate change in the southern Indian Ocean

    Science.gov (United States)

    Michael, P. E.; Wilcox, C.; Tuck, G. N.; Hobday, A. J.; Strutton, P. G.

    2017-06-01

    Climate change is projected to continue shifting the distribution of marine species, leading to changes in local assemblages and different interactions with human activities. With regard to fisheries, understanding the relationship between fishing fleets, target species catch per unit effort (CPUE), and the environment enhances our ability to anticipate fisher response and is an essential step towards proactive management. Here, we explore the potential impact of climate change in the southern Indian Ocean by modelling Japanese and Taiwanese pelagic longline fleet dynamics. We quantify the mean and variability of target species CPUE and the relative value and cost of fishing in different areas. Using linear mixed models, we identify fleet-specific effort allocation strategies most related to observed effort and predict the future distribution of effort and tuna catch under climate change for 2063-2068. The Japanese fleet's strategy targets high-value species and minimizes the variability in CPUE of the primary target species. Conversely, the Taiwanese strategy indicated flexible targeting of a broad range of species, fishing in areas of high and low variability in catch, and minimizing costs. The projected future mean and variability in CPUE across species suggest a slight increase in CPUE in currently high CPUE areas for most species. The corresponding effort projections suggest a slight increase in Japanese effort in the western and eastern study area, and Taiwanese effort increasing east of Madagascar. This approach provides a useful method for managers to explore the impacts of different fishing and fleet management strategies for the future.

  19. Information Technology Management System: an Analysis on Computational Model Failures for Fleet Management

    Directory of Open Access Journals (Sweden)

    Jayr Figueiredo de Oliveira

    2013-10-01

    Full Text Available This article proposes an information technology model to evaluate fleet management failure. Qualitative research done by a case study within an Interstate Transport company in a São Paulo State proposed to establish a relationship between computer tools and valid trustworthy information needs, and within an acceptable timeframe, for decision making, reliability, availability and system management. Additionally, the study aimed to provide relevant and precise information, in order to minimize and mitigate failure actions that may occur, compromising all operational organization base functioning.

  20. Optimization and control method for smart charging of EVs facilitated by Fleet operator

    DEFF Research Database (Denmark)

    Hu, Junjie; You, Shi; Si, Chengyong

    2013-01-01

    the energy during the period of high electricity penetration and feed the electricity back into the grid when the demand is high or in situations of insufficient electricity generation. However, the extra loads created by increasing EVs may have adverse impacts on grid. These factors will bring new......-tion and the facilitator EV fleet operator are introduced. Secondly, control architec-tures and their integrations in term of electricity market and distribution grid are discussed. Then, data analysis of EVs including a battery model and driving pattern is presented. Further discussion is given on mathematical modelling...

  1. Transition of R&D into Operations at Fleet Numerical Meteorology and Oceanography Center

    Science.gov (United States)

    Clancy, R. M.

    2006-12-01

    The U.S. Navy's Fleet Numerical Meteorology and Oceanography Center (FNMOC) plays a significant role in the National capability for operational weather and ocean prediction through its operation of sophisticated global and regional meteorological and oceanographic models, extending from the top of the atmosphere to the bottom of the ocean. FNMOC uniquely satisfies the military's requirement for a global operational weather prediction capability based on software certified to DoD Information Assurance standards and operated in a secure classified computer environment protected from outside intrusion by DoD certified firewalls. FNMOC operates around-the-clock, 365 days per year and distributes products to military and civilian users around the world, both ashore and afloat, through a variety of means. FNMOC's customers include all branches of the Department of Defense, other government organizations such as the National Weather Service, private companies, a number of colleges and universities, and the general public. FNMOC employs three primary models, the Navy Operational Global Atmospheric Prediction System (NOGAPS), the Coupled Ocean/Atmosphere Mesoscale Prediction System (COAMPS), and the WaveWatch III model (WW3), along with a number of specialized models and related applications. NOGAPS is a global weather model, driving nearly all other FNMOC models and applications in some fashion. COAMPS is a high- resolution regional model that has proved to be particularly valuable for forecasting weather and ocean conditions in highly complex coastal areas. WW3 is a state-of-the-art ocean wave model that is employed both globally and regionally in support of a wide variety of naval operations. Other models support and supplement the main models with predictions of ocean thermal structure, ocean currents, sea-ice characteristics, and other data. Fleet Numerical operates at the leading edge of science and technology, and benefits greatly from collocation with its supporting

  2. Sustainable Federal Fleets: Deploying Electric Vehicles and Electric Vehicle Supply Equipment

    Energy Technology Data Exchange (ETDEWEB)

    2017-01-01

    The U.S. Department of Energy (DOE) Federal Energy Management Program (FEMP) helps federal agencies reduce petroleum consumption and increase alternative fuel use through its resources for Sustainable Federal Fleets. To assist agencies with the transition to plug-in electric vehicles (PEVs), including battery electric vehicles (BEVs) and plug-in hybrid electric vehicles (PHEVs), FEMP offers technical guidance on electric vehicle supply equipment (EVSE) installations and site-specific planning through partnerships with the National Renewable Energy Laboratory's (NREL's) EVSE Tiger Teams.

  3. Efeito terapêutico da aplicação intra-ocular de ozônio em modelo experimental de endoftalmite por Staphylococcus epidermidis em coelhos Therapeutic effect of intraocular application of ozone in experimental Staphylococcus epidermidis endophthalmitis

    Directory of Open Access Journals (Sweden)

    Jonathan Clive Lake

    2004-08-01

    Full Text Available OBJETIVO: Avaliar a ação antiinflamatória e antimicrobiana da injeção intravítrea de ozônio diluído em solução salina balanceada (BSS em modelo experimental de endoftalmite por Staphylococcus epidermidis. MÉTODOS: Dezessete coelhos da raça Nova Zelândia receberam inoculação no olho direito de 0,1 ml de uma solução de 10(5 bactérias (S. epidermidis por ml para indução de endoftalmite. Sete coelhos receberam injeção intravítrea de 0,1ml de BSS como controle e os outros dez coelhos receberam injeção intravítrea de 0,1ml de BSS com ozônio diluído a 2 ppm. Os dados foram quantificados de acordo com parâmetros clínicos e histopatológicos e computados numericamente para cada coelho após 24 horas de evolução. As médias e os desvios-padrão (DP foram calculados e comparados por meio de teste t com duas amostras. RESULTADOS: A média da quantificação clínica dos olhos dos coelhos do grupo controle foi de 11,14 com DP de 1,04. A média clínica dos olhos tratados com ozônio foi de 4,90 com DP de 1,29. A média dos olhos tratados com ozônio foi significativamente menor do que os não tratados (PPURPOSE: To evaluate the anti-inflammatory and antimicrobial effect of intraocular ozone diluted in BSS on experimental Staphylococcus epidermidis endophthalmitis. METHODS: We injected 0.1ml of S. epidermidis colonies (10(5 colonies/ml to produce experimental endophthalmitis in the right eyes of 17 New Zealand rabbits. Seven rabbits were assigned to a control group and received an intravitreal injection of 0.1ml BSS. Ten rabbits were treated with intravitreal injection of 0.1ml BSS-diluted ozone (2 ppm. Data were distributed according to clinical and histological findings after 24 hours, each rabbit receiving a score. Mean scores and standard deviations were compared using the two-sample t test. RESULTS: Mean clinical score for the control group was 11.14 ± 1.04. Mean clinical score for the treated group was 4.90 ± 1

  4. Análise comparativa dos parâmetros redox em diferentes cultivares de Phaseolus vulgaris L. que apresentam susceptibilidade diferenciada à exposição ao ozônio

    OpenAIRE

    Fernanda Freitas Caregnato

    2013-01-01

    O ozônio (O3) troposférico pode ser considerado um dos poluentes atmosféricos mais tóxicos da atualidade. Nas espécies vegetais o O3 penetra através dos estômatos, interferindo nos processos fisiológicos, metabólicos e celulares das plantas. Os efeitos negativos do O3 são desencadeados devido ao fato deste ser um potente agente oxidante capaz de gerar espécies ativas de oxigênio (EAO) no meio intracelular. No entanto, as investigações sobre os impactos da exposição ao O3 na fisiologia e produ...

  5. Mathematical optimization techniques for managing selective catalytic reduction for a fleet of coal-fired power plants

    Science.gov (United States)

    Alanis Pena, Antonio Alejandro

    Major commercial electricity generation is done by burning fossil fuels out of which coal-fired power plants produce a substantial quantity of electricity worldwide. The United States has large reserves of coal, and it is cheaply available, making it a good choice for the generation of electricity on a large scale. However, one major problem associated with using coal for combustion is that it produces a group of pollutants known as nitrogen oxides (NO x). NOx are strong oxidizers and contribute to ozone formation and respiratory illness. The Environmental Protection Agency (EPA) regulates the quantity of NOx emitted to the atmosphere in the United States. One technique coal-fired power plants use to reduce NOx emissions is Selective Catalytic Reduction (SCR). SCR uses layers of catalyst that need to be added or changed to maintain the required performance. Power plants do add or change catalyst layers during temporary shutdowns, but it is expensive. However, many companies do not have only one power plant, but instead they can have a fleet of coal-fired power plants. A fleet of power plants can use EPA cap and trade programs to have an outlet NOx emission below the allowances for the fleet. For that reason, the main aim of this research is to develop an SCR management mathematical optimization methods that, with a given set of scheduled outages for a fleet of power plants, minimizes the total cost of the entire fleet of power plants and also maintain outlet NO x below the desired target for the entire fleet. We use a multi commodity network flow problem (MCFP) that creates edges that represent all the SCR catalyst layers for each plant. This MCFP is relaxed because it does not consider average daily NOx constraint, and it is solved by a binary integer program. After that, we add the average daily NOx constraint to the model with a schedule elimination constraint (MCFPwSEC). The MCFPwSEC eliminates, one by one, the solutions that do not satisfy the average daily

  6. CERN’s automobile fleet turns a brighter shade of green

    CERN Document Server

    Anaïs Vernède

    2011-01-01

    CERN is partnering with Services Industriels de Genève (SIG) in an environmentally friendly initiative to acquire cars running on natural gas. The first forty vehicles will arrive at CERN around mid-February, and enter service in March.   On 3 February 2011 CERN, represented by Steve Myers at the FEDRE Forum, was awarded the mobility prize. CERN currently has approximately 800 vehicles in use at its different sites. Véronique Marchal, head of Site Services in the GS Department, explains: “Our fleet includes a great variety of vehicles, from small Category A cars for staff to utility/transport vehicles. The decision to acquire cars running on natural gas is part of our strategy of diversifying CERN's automobile fleet so as to make it more environmentally friendly.” The new cars are actually bi-fuel, which means that they will have two tanks: one for regular fuel and one for compressed natural gas (CNG). “This kind of car is a little more exp...

  7. Flight Dynamics Operations Management of the Large and Heterogeneous Eutelsat Fleet of Commercial Satellites

    Science.gov (United States)

    Bellido, E.

    The EUTELSAT FDU (Flight Dynamics Unit) manages the resources to perform the typical activities of the large satellite operators and faces the usual difficulties raising from a vast and heterogeneous fleet. At present 20 satellites from 9 different platforms/sub-platforms are controlled from our Satellite Control Centre. The FDU was created in 2002 with the aim to respond to the operational needs of a growing fleet in terms of number of satellites and activities. It is at present composed of 6 engineering staff with the objective to provide operations service covering the whole lifecycle of the satellites from the procurement phase till the decommissioning. The most demanding activity is the daily operations, which must ensure maximum safety and continuity of service with the highest efficiency. Solutions have been applied from different areas: management, structure, operations organisation, processes, facilities, quality standards, etc. In addition to this, EUTELSAT is a growing communications operator and the FDU needs to contribute to the global objectives of the company. This paper covers our approach.

  8. Mobile source CO2 mitigation through smart growth development and vehicle fleet hybridization.

    Science.gov (United States)

    Stone, Brian; Mednick, Adam C; Holloway, Tracey; Spak, Scott N

    2009-03-15

    This paper presents the results of a study on the effectiveness of smart growth development patterns and vehicle fleet hybridization in reducing mobile source emissions of carbon dioxide (CO2) across 11 major metropolitan regions of the Midwestern U.S. over a 50-year period. Through the integration of a vehicle travel activity modeling framework developed by researchers atthe Oak Ridge National Laboratory with small area population projections, we model mobile source emissions of CO2 associated with alternative land development and technology change scenarios between 2000 and 2050. Our findings suggest that under an aggressive smart growth scenario, growth in emissions expected to occur under a business as usual scenario is reduced by 34%, while the full dissemination of hybrid-electric vehicles throughout the light vehicle fleet is found to offset the expected growth in emissions by 97%. Our results further suggest that high levels of urban densification could achieve reductions in 2050 CO2 emissions equivalent to those attainable through the full dissemination of hybrid-electric vehicle technologies.

  9. Transition to a hydrogen fuel cell transit bus fleet for Canadian urban transit system

    International Nuclear Information System (INIS)

    Ducharme, P.

    2004-01-01

    'Full text:' The Canadian Transportation Fuel Cell Alliance (CTFCA), created by the Canadian Government as part of its 2000 Climate Change Action Plan, has commissioned MARCON-DDM's Hydrogen Intervention Team (HIT) to provide a roadmap for urban transit systems that wish to move to hydrogen fuel cell-powered bus fleets. HIT is currently in the process of gathering information from hydrogen technology providers, bus manufacturers, fuelling system providers and urban transit systems in Canada, the US and Europe. In September, HIT will be in a position to provide a preview of its report to the CTFCA, due for October 2004. The planned table of contents includes: TOMORROW'S FUEL CELL (FC) URBAN TRANSIT BUS - Powertrain, on-board fuel technologies - FC engine system manufacturers - Bus technical specifications, performances, operating characteristics - FC bus manufacturers TOMORROW'S FC TRANSIT PROPERTY - Added maintenance, facilities and fuelling infrastructure requirements - Supply chain implications - Environmental and safety issues - Alternative operational concepts PATHWAYS TO THE FUTURE - Choosing the future operational concept - 'Gap' assessment - how long from here to there? - Facilities and fleet adjustments, including fuelling infrastructure - Risk mitigation, code compliance measures TRANSITIONAL CONSIDERATIONS - Cost implications - Transition schedule (author)

  10. A Column Generation for the Heterogeneous Fixed Fleet Open Vehicle Routing Problem

    Directory of Open Access Journals (Sweden)

    Majid Yousefikhoshbakht

    2017-07-01

    Full Text Available This paper addressed the heterogeneous fixed fleet open vehicle routing problem (HFFOVRP, in which the vehicles are not required to return to the depot after completing a service. In this new problem, the demands of customers are fulfilled by a heterogeneous fixed fleet of vehicles having various capacities, fixed costs and variable costs. This problem is an important variant of the open vehicle routing problem (OVRP and can cover more practical situations in transportation and logistics. Since this problem belongs to NP-hard Problems, An approach based on column generation (CG is applied to solve the HFFOVRP. A tight integer programming model is presented and the linear programming relaxation of which is solved by the CG technique. Since there have been no existing benchmarks, this study generated 19 test problems and the results of the proposed CG algorithm is compared to the results of exact algorithm. Computational experience confirms that the proposed algorithm can provide better solutions within a comparatively shorter period of time.

  11. Transport control of forest fuels by fleet manager, mobile terminals and GPS

    International Nuclear Information System (INIS)

    Sikanen, Lauri; Asikainen, Antti; Lehikoinen, Mikko

    2005-01-01

    Chip transportation is undertaken by private transportation enterprises that are contracted by a larger wood fuel trader. Compared with industrial roundwood supply the volumes and value of wood fuel supply are markedly lower. As a result, the possibilities to invest in information systems for transport management are limited. New portable, wireless communication techniques and internet-based systems for fleet management enable more cost-efficient control systems with low investment costs in hard and software and also low operating costs. Mobile handsets with GPS, digital road map display and global positioning systems (GPS) or general purpose radio service data transfer protocol can be used as mobile terminals in chippers and trucks. In addition, transport management personnel can use them to locate in-forest wood fuel storage piles into the map database as well as point suitable routing to the storage piles. In a pilot study, conducted in Central Finland, an internet based management tool, Arbonaut Fleet ManagerTM, was tailored for forest fuel supply chain management and trailed for three months. It was found that use of mobile handsets with GPS and map display assisted especially in exact location of in-forest wood fuel storage piles by managers. They assisted also trucks and chippers in navigation to storages and landings, but the screen was too small for reading during driving. The management system was found helpful in stock accounting and GPS-based vehicle tracking gave transportation distances directly for invoicing

  12. GeoTrac -- Producing fleet communications, asset control solutions and logistic tools

    Energy Technology Data Exchange (ETDEWEB)

    Anon

    2005-04-01

    GEOTrac, a Calgary-based company, that offers innovative solutions utilizing GPS, satellite and geomatic data technology in the areas of asset and fleet management, logistic tools and integrated solutions, is reviewed. The two-year-old company with a development staff of 12, has contracts with major energy companies in the Middle East, North America, Africa, China and Mexico. By integrating 30 low-earth orbit satellites, operated by Orbcomm, with GPS, leading -edge mapping and geomatic data technologies, GEOTrac provides world class communications and control solutions for assets in both urban and rural locations. The system is capable of providing up-to-the minute location data about every remote asset or vehicle in a fleet, including status reports, dispatch instructions and driving directions. Logistic tools and integrated solutions available from GEOTrac enable the simple management and visibility of equipment, vessels, containers and cargo within and into remote countries, as well as making possible remote diagnostic and storage tank level readings. In another development, advanced digital map solutions provide a suite of maps and geographic technologies for on-road and off-road driving directions, for identifying the location of services and destinations, and for creating strategic advantages by keeping up-to-date with specific company or other oilfield locations and activities.

  13. Preliminary investigation Area 12 fleet operations steam cleaning discharge area Nevada Test Site

    International Nuclear Information System (INIS)

    1996-07-01

    This report documents the characterization activities and findings of a former steam cleaning discharge area at the Nevada Test Site. The former steam cleaning site is located in Area 12 east of Fleet Operations Building 12-16. The characterization project was completed as a required condition of the ''Temporary Water Pollution Control Permit for the Discharge From Fleet Operations Steam Cleaning Facility'' issued by the Nevada Division of Environmental Protection. The project objective was to collect shallow soil samples in eight locations in the former surface discharge area. Based upon field observations, twelve locations were sampled on September 6, 1995 to better define the area of potential impact. Samples were collected from the surface to a depth of approximately 0.3 meters (one foot) below land surface. Discoloration of the surface soil was observed in the area of the discharge pipe and in localized areas in the natural drainage channel. The discoloration appeared to be consistent with the topographically low areas of the site. Hydrocarbon odors were noted in the areas of discoloration only. Samples collected were analyzed for bulk asbestos, Toxicity Characteristic Leaching Procedure (TCLP) metals, total petroleum hydrocarbons (TPHs), volatile organic compounds (VOCs), semi-volatile organic compounds (Semi-VOCs), and gamma scan

  14. Persistent Identifiers for Field Expeditions: A Next Step for the US Oceanographic Research Fleet

    Science.gov (United States)

    Arko, Robert; Carbotte, Suzanne; Chandler, Cynthia; Smith, Shawn; Stocks, Karen

    2016-04-01

    Oceanographic research cruises are complex affairs, typically requiring an extensive effort to secure the funding, plan the experiment, and mobilize the field party. Yet cruises are not typically published online as first-class digital objects with persistent, citable identifiers linked to the scientific literature. The Rolling Deck to Repository (R2R; info@rvdata.us) program maintains a master catalog of oceanographic cruises for the United States research fleet, currently documenting over 6,000 expeditions on 37 active and retired vessels. In 2015, R2R started routinely publishing a Digital Object Identifier (DOI) for each completed cruise. Cruise DOIs, in turn, are linked to related persistent identifiers where available including the Open Researcher and Contributor ID (ORCID) for members of the science party, the International Geo Sample Number (IGSN) for physical specimens collected during the cruise, the Open Funder Registry (FundRef) codes that supported the experiment, and additional DOIs for datasets, journal articles, and other products resulting from the cruise. Publishing a persistent identifier for each field expedition will facilitate interoperability between the many different repositories that hold research products from cruises; will provide credit to the investigators who secured the funding and carried out the experiment; and will facilitate the gathering of fleet-wide altmetrics that demonstrate the broad impact of oceanographic research.

  15. Actual car fleet emissions estimated from urban air quality measurements and street pollution models

    International Nuclear Information System (INIS)

    Palmgren, F.; Berkowicz, R.; Hertel, O.; Ziv, A.

    1999-01-01

    A method to determine emissions from the actual car fleet under realistic driving conditions has been developed. The method is based on air quality measurements, traffic counts and inverse application of street air quality models. Many pollutants are of importance for assessing the adverse impact of the air pollution, e.g. NO 2 , CO, lead, VOCs and particulate matter. Aromatic VOCs are of special great concern due to their adverse health effects. Measurements of benzene, toluene and xylenes were carried out in central Copenhagen since 1994. Significant correlation was observed between VOCs and CO concentrations, indicating that the petrol engine vehicles are the major sources of VOC air pollution in central Copenhagen. Hourly mean concentrations of benzene were observed to reach values of up to 20 ppb, what is critically high according to the WHOs recommendations. Based on inverse model calculation of dispersion of pollutants in street canyons, an average emission factor of benzene for the fleet of petrol fuelled vehicles was estimated to be 0.38 g/km in 1994 and 0.11 in 1997. This decrease was caused by the reduction of benzene content in Danish petrol since summer 1995 and increasing percentage of cars equipped with three-way catalysts. The emission factors for benzene for diesel-fuelled vehicles were low

  16. Tools for improving safety management in the Norwegian Fishing Fleet occupational accidents analysis period of 1998-2006.

    Science.gov (United States)

    Aasjord, Halvard L

    2006-01-01

    Reporting of human accidents in the Norwegian Fishing Fleet has always been very difficult because there has been no tradition in making reports on all types of working accidents among fishermen, if the accident does not seem to be very serious or there is no economical incentive to report. Therefore reports are only written when the accidents are serious or if the fisherman is reported sick. Reports about an accident are sent to the insurance company, but another report should also be sent to the Norwegian Maritime Directorate (NMD). Comparing of data from one former insurance company and NMD shows that the real numbers of injuries or serious accidents among Norwegian fishermen could be up to two times more than the numbers reported to NMD. Special analyses of 1690 accidents from the so called PUS-database (NMD) for the period 1998-2002, show that the calculated risk was 23.6 accidents per 1000 man-years. This is quite a high risk level, and most of the accidents in the fishing fleet were rather serious. The calculated risks are highest for fishermen on board the deep sea fleet of trawlers (28.6 accidents per 1000 man-years) and also on the deep sea fleet of purse seiners (28.9 accidents per 1000 man-years). Fatal accidents over a longer period of 51.5 years from 1955 to 2006 are also roughly analysed. These data from SINTEF's own database show that the numbers of fatal accidents have been decreasing over this long period, except for the two periods 1980-84 and 1990-94 where we had some casualties with total losses of larger vessels with the loss of most of the crew, but also many others typical work accidents on smaller vessels. The total numbers of registered Norwegian fishermen and also the numbers of man-years have been drastically reduced over the 51.5 years from 1955 to 2006. The risks of fatal accidents have been very steady over time at a high level, although there has been a marked risk reduction since 1990-94. For the last 8.5-year period of January 1998

  17. Influence of fleet renewal and trawl development on landings per unit effort of the Danish northern shrimp (Pandalus borealis) fishery

    DEFF Research Database (Denmark)

    Eigaard, Ole Ritzau; Munch-Petersen, Sten

    2011-01-01

    Recent stock assessments of the Pandalus stock in the Skagerrak (ICES Division IIIa) and the Norwegian Deep (Division IVa east) have relied largely on a time-series of landings per unit effort (lpue) calculated from Danish logbook data. Because of fleet renewal and trawl-size changes, the relatio......Recent stock assessments of the Pandalus stock in the Skagerrak (ICES Division IIIa) and the Norwegian Deep (Division IVa east) have relied largely on a time-series of landings per unit effort (lpue) calculated from Danish logbook data. Because of fleet renewal and trawl-size changes...

  18. AVTA Federal Fleet PEV Readiness Data Logging and Characterization Study for the National Park Service: Sleeping Bear Dunes National Lakeshore

    Energy Technology Data Exchange (ETDEWEB)

    Schey, Stephen [Idaho National Lab. (INL), Idaho Falls, ID (United States); Francfort, Jim [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2014-11-01

    This report focuses on the Sleeping Bear Dunes National Lakeshore (SLBE) fleet to identify daily operational characteristics of select vehicles and report findings on vehicle and mission characterizations to support the successful introduction of plug-in electric vehicles (PEVs) into the agencies’ fleets. Individual observations of these selected vehicles provide the basis for recommendations related to electric vehicle adoption and whether a battery electric vehicle (BEV) or plug-in hybrid electric vehicle (PHEV) (collectively plug-in electric vehicles, or PEVs) can fulfill the mission requirements.

  19. AVTA Federal Fleet PEV Readiness Data Logging and Characterization Study for Department of Veterans Affairs – VA Manhattan Campus

    Energy Technology Data Exchange (ETDEWEB)

    Stephen Schey; Jim Francfort

    2014-10-01

    This report focuses on the Department of Veterans Affairs, VA Manhattan Campus (VA- Manhattan) fleet to identify the daily operational characteristics of select vehicles and report findings on vehicle and mission characterizations to support successful introduction of plug-in electric vehicles (PEVs) into the agency’s fleet. Individual observations of these selected vehicles provide the basis for recommendations related to electric vehicle adoption and whether a battery electric vehicle or plug-in hybrid electric vehicle (collectively called PEVs) can fulfill the mission requirements.

  20. AVTA Federal Fleet PEV Readiness Data Logging and Characterization Study for Department of Health and Human Services – ASPR

    Energy Technology Data Exchange (ETDEWEB)

    Schey, Steve [Idaho National Lab. (INL), Idaho Falls, ID (United States); Francfort, Jim [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-06-01

    This report focuses on the Department of Health and Human Services, Assistant Secretary for Preparedness and Response fleet to identify daily operational characteristics of select vehicles and report findings on vehicle and mission characterizations to support the successful introduction of PEVs into the agency’s fleet. Individual observations of these selected vehicles provide the basis for recommendations related to electric vehicle adoption and whether a battery electric vehicle or plug-in hybrid electric vehicle (collectively referred to as PEVs) can fulfill the mission requirements.