Full Text Available Background and Aim: Due to the high prevalence and worldwide distribution of Enterobius vermicularis and its readily transmission among children, parents' knowledge and health education have an important role in restriction and infection control. This study was performed to evaluate the girl students' knowledge about transmission, symptoms, and prevention of oxyuriasis in 2012–2013 in Kashan, Central Iran. Materials and Methods: In this cross-sectional study, a total of 500 students in three levels of middle schools were studied. To evaluate the students' knowledge level of oxyuriasis, the standardized questionnaire was distributed and completed. Data were analyzed using statistical software SPSS. Results: Among three levels of students of middle schools, 67.6% of students had average awareness (information, 17.4% had weak information, and 15% had high information. In the assessment of the level of awareness in the first-level students, 23.41% of the students had weak information, 66.4% had average information, and 10.12% had high information. In the second-level students, 12.57% had weak information, 73.14% had average information, and 14.28% had high information. In the third-level middle-school students, 16.76% of students had weak knowledge, 62.87% had average knowledge, and 20.35% had high knowledge. Conclusion: The result of this study shows that knowledge of middle-school students about this infection is good but not sufficient, and it is necessary for teachers and health officers in schools to increase hygienic knowledge of the students and to train about this infection to decrease the damage of personal and social problems.
Full Text Available O encontro de parasitos em material arqueológico tem permitido o acompanhamento da dispersão de agentes infecciosos e seus hospedeiros humanos no passado. Através desses achados, pôde-se retomar teorias propostas no início do século sobre contatos transpacíficos de populações asiáticas com a América do Sul. Assim foi feito, por exemplo, no caso do encontro de ovos de ancilostomídeos em material arqueológico datado de até sete mil anos antes do presente. O aumento da produção científica na área torna necessária, agora, a realização de trabalhos de síntese que avaliem o estado da arte e proponham modelos paleoepidemiológicos compreensíveis para a dispersão pré-histórica das parasitoses humanas. Nesse sentido, foi feito o presente trabalho, a partir dos recentes achados de ovos de Enterobius vermicularis em material arqueológico nas Américas. Diferentemente da infecção por ancilostomídeos, a oxiuríase não necessita de passagem pelo solo para a transmissão de um hospedeiro a outro, logo, sua persistência em uma dada população humana independe das condições climáticas. Poderia, portanto, ter sido trazida do velho para o novo continente, inclusive pelas migrações humanas que atravessaram o estreito de Bering. Tal fato pode explicar a maior dispersão geográfica dos achados e sua disseminação na América do Norte, desde dez mil anos até os tempos atuais. Na América do Sul, por outro lado, os achados arqueológicos só confirmaram sua existência ao longo da região andina, com achados no Chile e no Norte da Argentina. No Brasil, apesar do grande número de amostras examinadas, não foram encontrados ovos de Enterobius vermicularis em coprólitos. No presente trabalho, discutem-se modelos explicativos para a distribuição conhecida dessa parasitose em populações pré-históricas.Parasite findings in archeological material have made it possible to trace the dispersion of infectious agents and their human hosts in ancient times. These findings allow us to re-examine theories proposed at the beginning of the century concerning transpacific contacts that Asian populations may have had with South America. This has been the case, for example, with hookworm eggs found in archeological material dating up to 7,000 years before present. Because of the increase in scientific production in this area, it has now become necessary to undertake syntheses that assess the state of the art and propose workable paleoepidemological models of the prehistoric dispersion of human parasitoses. Based on findings of Enterobius vermicularis eggs in archeological material in the Americas, the present study is an effort in this direction. Unlike the hookworm, the pinworm does not require a soil cycle in order to be transmitted from one host to another, thereby meaning that its persistence in a given human population does not depend on climatic conditions. Thus, it could have been brought from the old to the new continent, possibly by human migrations across the Bering Strait. This may explain the greater geographical dispersion and dissemination of these findings in North America from 10,000 yrs B.P. till today. In South America, on the other hand, archeological findings have only confirmed existence of Enterobius vermicularis eggs within the Andean region, with findings located specifically in Chile and northern Argentina. Although a large number of samples have been examined, no such eggs have been found in coprolites in Brazil. The paper discusses models that account for the known distribution of this parasitosis in prehistoric populations.
Jun 13, 2017 ... Oxyuriasis's symptoms are extremely diverse in children, ranging from nausea, diarrhea, insomnia, irritability, recurrent cellulitis, loss of appetite, nightmares and endometritis. Here we report a curious case of oxyuriasis in the settings of a refugee camp in Greece. The patient was a. 10-year old Syrian ...
Oxyuriasis test; Enterobiasis test; Tape test ... diagnose this infection is to do a tape test. The best time to do this is in ... lay their eggs at night. Steps for the test are: Firmly press the sticky side of a ...
Smith, H.V.; Quinn, R.; Bruce, R.G.; Girdwood, R.W.A.
A paper radioimmunosorbent test (PRIST) was shown to be sensitive and reproducible when used with excretory/secretory antigen of Toxocara canis second stage larvae. Whatman No. 50 filter paper (5 mm discs) gave the most consistent and clear results with antigen at a concentration of 100 μg/ml, and could be stored for up to 3 weeks in vacuo at -70 0 C. Antigen coated discs were incubated with test sera at 1 : 10 dilution for 3 h at room temperature (21 0 C), reacted with [ 125 I]anti-human IgG for 1 h and counts determined in a gamma counter. Sera from patients with fascioliasis, taeniasis, schistosomiasis, oxyuriasis, trichinellosis and ancyclostomiasis gave counts similar to cord serum controls. Sera from patients with ascariasis gave counts of up to twice as great as controls, but sera from patients with toxicariasis produced counts of 7,000-13,000, a 4-6 fold increase. (Auth.)
Full Text Available Both the clinical and the histopathological diagnostic difficulties of oxyuriasis in unusual sites and their importance from a clinical point of view are pointed out. The authors report two ectoptic cases of enterobiasis observed in Northern Italy, one located in a fallopian tube of a 57-year-old woman and the other in a perianal subcutaneous tissue of a 59-year-old man, mimicking tumor-like lesions. The authors take advantage of the occasion to focus the attention of the medical world on this subject, lamenting the scarce importance given to this parasitosis in university courses of medical schools and in medical textbooks as it is incorrectly considered “out-of-fashion.”
In this paper the literature on helminthiasis in Iran is reviewed and the distribution, prevalence and intensity of infections caused by helminths are discussed. A comparison of prevalence and intensity of infections was accomplished in the recent 10 years by the author. Hookworm infections are found mostly in the north along the Caspian sea, but its prevalence is declining. Trichostrongylus are found in Iran with a high prevalence in central country. Schistosoma haematobium is found in one Province in the south, but its prevalence is declining because of large scale control activities. The Guinea worm rarely occurs in Iran. The prevalence of Fasciola hepatica is high among livestock and is occasionally found in man. Taenia saginata is found in most parts of Iran. Taenia solium is seldom found in animals with a prevalence of 0.03%. Trichinella spiralis is infrequently found in animals. Dioctophyma renale is found in straying dogs in the north or Iran, the percentage is 13-35%. Oxyuriasis is widespread among children.
Full Text Available Research conducted on the parasitological pollution in some digestive helminthosis in horses, (parascaridiosis,strongillidosis and oxyuriasis, made in January 2011 in the village of Crasna, Salaj county on a herd of 72 horsesfrom households (40 adults and 32 young horses, using diagnostic methods ovohelmintoscopics with enrichment offaeces taken (method Willis and the scaling technique of curettage in anal pit area, revealed a different incidence ofthe main digestive helmintoze studied in correlation with age category and area of origin of the samples. Thus, meanparascaridiosis values are of 12.5% in adults and of 34.0% in young horses and riding in strongillidosis to an averageof 82.5% in adult horses and, respectively, to 34.5% in young horses. In exchange for pinworm infection extensivityof parasitism in adult horses is 18.0% and respectively, 15.0% in young horses. Linked to the intensity of parasitismin helmintosis taken in study, the data obtained reveals different levels of pollution coproparasitologic Eggtester, incorrelation with the area of origin and age. Thus, adult and youth horse parascaridiosis, dominates the low andmedium infestations (100% and in adult horses strongilidosis dominate the medium infestations (84.0% and lowinfestations (75% in young horses.
Duan, Qiwen; Liu, Yanfeng; Booth, Carmen J; Rockwell, Sara
Pinworm infection (oxyuriasis) is a common problem in rodent colonies. Facility-wide prophylactic treatment of all mice with a diet containing therapeutic levels of fenbendazole for several weeks is often used to control pinworm outbreaks. We examined the effect of feeding a therapeutic diet containing 150 ppm fenbendazole on the growth of EMT6 mouse mammary tumors implanted into BALB/c Rw mice. Mice were randomized to receive either a fenbendazole-containing or control diet for 1 wk before tumor cells were injected intradermally in the flanks and throughout tumor growth. Tumor growth was monitored by serial measurements of tumor diameters from the time tumors became palpable until they reached 1000 mm3. The medicated diet did not alter tumor growth, invasion, or metastasis. When tumors reached volumes of approximately 100 mm3, some were irradiated locally with 10 Gy of X-rays. Irradiation significantly delayed tumor growth; fenbendazole did not alter the radiation-induced growth delay. However, cell culture studies showed that fenbendazole concentrations not far above those expected in the tissues of mice on this diet altered the growth of the tumor cells in culture. Recent data from other laboratories also have demonstrated effects of fenbendazole that could complicate experiments. Care should therefore be exercised in deciding whether chow containing fenbendazole should be administered to mouse colonies being used in cancer research.
CARACTERÍSTICAS CLÍNICAS Y EPIDEMIOLÓGICAS DE ENTEROBIASIS EN NIÑOS ESCOLARIZADOS DE UNA ZONA RURAL DEL ESTADO FALCÓN, VENEZUELA | CLINICAL AND EPIDEMIOLOGICAL CHARACTERISTICS OF ENTEROBIASIS IN RURAL SCHOLAR CHILDREN FROM FALCON STATE, VENEZUELA
Full Text Available The enteric helminth Enterobius vermicularis is the causal agent of enterobiasis or oxyuriasis, which is most prevalent in children. A study was designed to determine the prevalence and clinical and epidemiological profiles of enterobiasis in preschool and elementary school children of El Paso Acurigua, Falcon state, Venezuela. The descriptive, prospective and cross-sectional survey was performed from November 2006 to April 2007, in a total of 82 children. Probable risk factors for enterobiasis were identified by using epidemiological questionnaires. Symptoms associated to enterobiasis were determined by means of clinical examination. The diagnosis of pinworm infection was made by adhesive cellulose tape. The overall prevalence of enterobiasis was 40.24% (33/82, with significantly higher percentages of infection in boys (p = 0.001. Anal pruritus (p = 0.001, restlessness (p = 0.002, bruxism (p < 0.000 and abdominal pain (p = 0.02 were the symptoms significantly associated with enterobiasis. Multiple logistic regression analysis allowed the determination of independent potential risk factors for transmission of this enterohelminthiasis: anal pruritus [Odds Ratio (OR = 3.6], onycophagy (OR = 2.8, dirty fingernails (OR = 6.3, pet playing (OR = 2.0, defecation on septic tank (OR = 2.3 and overcrowding (≥ 6 persons/household: OR = 19.8; ≥ 3 persons/room: OR = 1.8. Enterobiasis remains highly prevalent among school children from “El Paso Acurigua”. Thus, improving standards of living and personal-community hygiene as well as education campaigns, are advised.
Mário Henrique M Barros
Full Text Available Macrophage polarization is increasingly recognised as an important pathogenetic factor in inflammatory and neoplastic diseases. Proinflammatory M1 macrophages promote T helper (Th 1 responses and show tumoricidal activity. M2 macrophages contribute to tissue repair and promote Th2 responses. CD68 and CD163 are used to identify macrophages in tissue sections. However, characterisation of polarised macrophages in situ has remained difficult. Macrophage polarisation is regulated by transcription factors, pSTAT1 and RBP-J for M1, and CMAF for M2. We reasoned that double-labelling immunohistochemistry for the detection of macrophage markers together with transcription factors may be suitable to characterise macrophage polarisation in situ. To test this hypothesis, we have studied conditions associated with Th1- and Th2-predominant immune responses: infectious mononucleosis and Crohn's disease for Th1 and allergic nasal polyps, oxyuriasis, wound healing and foreign body granulomas for predominant Th2 response. In all situations, CD163+ cells usually outnumbered CD68+ cells. Moreover, CD163+ cells, usually considered as M2 macrophages, co-expressing pSTAT1 and RBP-J were found in all conditions examined. The numbers of putative M1 macrophages were higher in Th1- than in Th2-associated diseases, while more M2 macrophages were seen in Th2- than in Th1 related disorders. In most Th1-related diseases, the balance of M1 over M2 cells was shifted towards M1 cells, while the reverse was observed for Th2-related conditions. Hierarchical cluster analysis revealed two distinct clusters: cluster I included Th1 diseases together with cases with high numbers of CD163+pSTAT1+, CD68+pSTAT1+, CD163+RBP-J+ and CD68+RBP-J+ macrophages; cluster II comprised Th2 conditions together with cases displaying high numbers of CD163+CMAF+ and CD68+CMAF+ macrophages. These results suggest that the detection of pSTAT1, RBP-J, and CMAF in the context of CD68 or CD163 expression is a
Barros, Mário Henrique M; Hauck, Franziska; Dreyer, Johannes H; Kempkes, Bettina; Niedobitek, Gerald
Macrophage polarization is increasingly recognised as an important pathogenetic factor in inflammatory and neoplastic diseases. Proinflammatory M1 macrophages promote T helper (Th) 1 responses and show tumoricidal activity. M2 macrophages contribute to tissue repair and promote Th2 responses. CD68 and CD163 are used to identify macrophages in tissue sections. However, characterisation of polarised macrophages in situ has remained difficult. Macrophage polarisation is regulated by transcription factors, pSTAT1 and RBP-J for M1, and CMAF for M2. We reasoned that double-labelling immunohistochemistry for the detection of macrophage markers together with transcription factors may be suitable to characterise macrophage polarisation in situ. To test this hypothesis, we have studied conditions associated with Th1- and Th2-predominant immune responses: infectious mononucleosis and Crohn's disease for Th1 and allergic nasal polyps, oxyuriasis, wound healing and foreign body granulomas for predominant Th2 response. In all situations, CD163+ cells usually outnumbered CD68+ cells. Moreover, CD163+ cells, usually considered as M2 macrophages, co-expressing pSTAT1 and RBP-J were found in all conditions examined. The numbers of putative M1 macrophages were higher in Th1- than in Th2-associated diseases, while more M2 macrophages were seen in Th2- than in Th1 related disorders. In most Th1-related diseases, the balance of M1 over M2 cells was shifted towards M1 cells, while the reverse was observed for Th2-related conditions. Hierarchical cluster analysis revealed two distinct clusters: cluster I included Th1 diseases together with cases with high numbers of CD163+pSTAT1+, CD68+pSTAT1+, CD163+RBP-J+ and CD68+RBP-J+ macrophages; cluster II comprised Th2 conditions together with cases displaying high numbers of CD163+CMAF+ and CD68+CMAF+ macrophages. These results suggest that the detection of pSTAT1, RBP-J, and CMAF in the context of CD68 or CD163 expression is a suitable tool for