WorldWideScience

Sample records for optimize biosurfactant production

  1. Response surface optimization of the medium components for the production of biosurfactants by probiotic bacteria

    NARCIS (Netherlands)

    Rodrigues, L; Teixeira, J; Oliveira, R; van der Mei, HC

    Optimization of the medium for biosurfactants production by probiotic bacteria (Lactococcus lactis 53 and Streptococcus thermophilus A) was carried out using response surface methodology. Both biosurfactants were proved to be growth-associated, thus the desired response selected for the optimization

  2. Optimization of cultural conditions for biosurfactant production by Pleurotus djamor in solid state fermentation.

    Science.gov (United States)

    Velioglu, Zulfiye; Ozturk Urek, Raziye

    2015-11-01

    Being eco-friendly, less toxic, more biodegradable and biocompatible, biological surfactants have higher activity and stability compared to synthetic ones. In spite of the fact that there are abundant benefits of biosurfactants over the synthetic congeners, the problem related with the economical and large scale production proceeds. The utilization of several industrial wastes in the production media as substrates reduces the production cost. This current study aims optimization of biosurfactant production conditions by Pleurotus djamor, grown on sunflower seed shell, grape wastes or potato peels as renewable cheap substrates in solid state fermentation. After determination of the best substrate for biosurfactant production, we indicate optimum size and amount of solid substrate, volume of medium, temperature, pH and Fe(2+) concentrations on biosurfactant production. In optimum conditions, by reducing water surface tension to 28.82 ± 0.3 mN/m and having oil displacement diameter of 3.9 ± 0.3 cm, 10.205 ± 0.5 g/l biosurfactant was produced. Moreover, chemical composition of biosurfactant produced in optimum condition was determined by FTIR. Lastly, laboratory's large-scale production was carried out in optimum conditions in a tray bioreactor designed by us and 8.9 ± 0.5 g/l biosurfactant was produced with a significant surface activity (37.74 ± 0.3 mN/m). With its economical suggestions and applicability of laboratory's large-scale production, this work indicates the possibility of using low cost agro-industrial wastes as renewable substrates for biosurfactant production. Therefore, using economically produced biosurfactant will reduce cost in several applications such as bioremediation, oil recovery and biodegradation of toxic chemicals. Copyright © 2015 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  3. Interactive optimization of biosurfactant production by Paenibacillus alvei ARN63 isolated from an Iranian oil well.

    Science.gov (United States)

    Najafi, A R; Rahimpour, M R; Jahanmiri, A H; Roostaazad, R; Arabian, D; Soleimani, M; Jamshidnejad, Z

    2011-01-01

    The potential of an indigenous bacterial strain isolated from an Iranian oil field for the production of biosurfactant was investigated in this study. After isolation, the bacterium was characterized to be Paenibacillus alvei by biochemical tests and 16S ribotyping. The biosurfactant, which was produced by this bacterium, was able to lower the surface tension of media to 35 mN/m. Accordingly, thin layer chromatography (TLC) and FT-IR has been carried out to determine compositional analysis of the produced biosurfactant. After all the tests related to characterization of the biosurfactant produced by the isolated bacterium, it was characterized as lipopeptide derivative. The combination of central composite rotatable design (CCRD) and response surface methodology (RSM) was exploited to optimize biosurfactant production. Therefore, variations of four impressive parameters, pH, temperature, glucose and salinity concentrations were selected for optimization of growth conditions. The empirical model developed through RSM in terms of effective operational factors mentioned above was found to be adequate to describe the biosurfactant production. A maximum reduction in surface tension was obtained under the optimal conditions of 13.03 g/l glucose concentration, 34.76 °C, 51.39 g/l total salt concentration and medium pH 6.89. Copyright © 2010 Elsevier B.V. All rights reserved.

  4. Optimization and characterization of biosurfactant production from marine Vibrio sp. strain 3B-2

    Science.gov (United States)

    Hu, Xiaoke; Wang, Caixia; Wang, Peng

    2015-01-01

    A biosurfactant-producing bacterium, designated 3B-2, was isolated from marine sediment and identified as Vibrio sp. by 16S rRNA gene sequencing. The culture medium composition was optimized to increase the capability of 3B-2 for producing biosurfactant. The produced biosurfactant was characterized in terms of protein concentration, surface tension, and oil-displacement efficiency. The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate. Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm. Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation. PMID:26441908

  5. Enhancement of Bacillus subtilis Lipopeptide Biosurfactants Production through Optimization of Medium Composition and Adequate Control of Aeration

    OpenAIRE

    Ghribi, Dhouha; Ellouze-Chaabouni, Semia

    2011-01-01

    Interest in biosurfactants has increased considerably in recent years, as they are potentially used in many commercial applications in petroleum, pharmaceuticals, biomedical, and food processing industries. Since improvement of their production was of great importance to reduce the final coast, cultural conditions were analyzed to optimize biosurfactants production from Bacillus subtilis SPB1 strain. A high yield of biosurfactants was obtained from a culture of B. subtilis using carbohydrate ...

  6. Optimizing Carbon/Nitrogen Ratio for Biosurfactant Production by a Bacillus subtilis Strain

    Science.gov (United States)

    Fonseca, R. R.; Silva, A. J. R.; de Franca, F. P.; Cardoso, V. L.; Sérvulo, E. F. C.

    A Bacillus subtilis strain isolated from contaminated soil from a refinery has been screened for biosurfactant production in crystal sugar (sucrose) with different nitrogen sources (NaNO3' (NH4)2SO4' urea, and residual brewery yeast). The highest reduction in surface tension was achieved with a 48-h fermentation of crystal sugar and ammonium nitrate. Optimization of carbon/nitrogen ratio (3,9, and 15) and agitation rate (50, 150, and 250 rpm) for biosurfactant production was carried out using complete factorial design and response surface analysis. The condition of C/N 3 and 250 rpm allowed the maximum increase in surface activity of biosurfactant. A suitable model has been developed, having presented great accordance experimental data. Preliminary characterization of the bioproduct suggested it to be a lipopeptide with some isomers differing from those of a commercial surfactin.

  7. Optimization of environmental factors for improved production of rhamnolipid biosurfactant by Pseudomonas aeruginosa RS29 on glycerol.

    Science.gov (United States)

    Saikia, Rashmi Rekha; Deka, Suresh; Deka, Manab; Sarma, Hemen

    2012-08-01

    A biosurfactant producing Pseudomonas aeruginosa RS29 (identified on the basis of 16S rDNA analysis) with good foaming and emulsification properties has been isolated from crude oil contaminated sites. Optimization of different environmental factors was carried out with an objective to achieve maximum production of biosurfactant. Production of biosurfactant was estimated in terms of surface tension reduction and emulsification (E24) index. It was recorded that the isolated strain produced highest biosurfactant after 48 h of incubation at 37.5 °C, with a pH range of 7-8 and at salinity biosurfactant (Surface tension, 26.3 and 26.4 mN/m and E24 index, 80 and 79% respectively). The CMC of the biosurfactant was 90 mg/l. Maximum biomass (6.30 g/l) and biosurfactant production (0.80 g/l) were recorded at an optimal C/N ratio of 12.5. Biochemical analysis and FTIR spectra confirmed that the biosurfactant was rhamnolipid in nature. GC-MS analysis revealed the presence of C(8) and C(10) fatty acid components in the purified biosurfactant. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  8. Optimization and characterization of biosurfactant production from kitchen waste oil using Pseudomonas aeruginosa.

    Science.gov (United States)

    Chen, Chunyan; Sun, Ni; Li, Dongsheng; Long, Sihua; Tang, Xiaoyu; Xiao, Guoqing; Wang, Linyuan

    2018-03-16

    Kitchen waste oil (KWO) from catering industries or households was used as a low-cost carbon source for producing biosurfactants by self-isolated Pseudomonas aeruginosa. Fermentation performance with KWO was superior to those with four other carbon sources, with higher optical density (OD 600 ) of 2.33 and lower interfacial tension of 0.57 mN/m. Culture conditions for biosurfactant production were optimized, with optimal pH of 8.0 and nitrogen source concentration of 2.0 g/L, respectively. The results of infrared spectroscopy and liquid chromatography-mass spectrometry (LC-MS) showed that the biosurfactant was a mixture of six rhamnolipid congeners, among which Rha-Rha-C 10 -C 10 and Rha-C 10 -C 10 were the main components, with mass fraction of approximately 34.20 and 50.86%, respectively. The critical micelle concentration (CMC) obtained was 55.87 mg/L. In addition, the rhamnolipids exhibited excellent tolerance to temperature (20-100 °C), pH (6.0-12.0), and salinity (2-20%; w/v) in a wide range, thereby showing good stability to extreme environmental conditions. The rhamnolipids positively affected oil removal from oil sludge and KWO-contaminated cotton cloth, with removal rate of 34.13 and of 30.92%, respectively. Our results demonstrated that biosurfactant production from KWO was promising, with advantages of good performance, low cost and environmental safety.

  9. Enhancement of Bacillus subtilis Lipopeptide Biosurfactants Production through Optimization of Medium Composition and Adequate Control of Aeration.

    Science.gov (United States)

    Ghribi, Dhouha; Ellouze-Chaabouni, Semia

    2011-01-01

    Interest in biosurfactants has increased considerably in recent years, as they are potentially used in many commercial applications in petroleum, pharmaceuticals, biomedical, and food processing industries. Since improvement of their production was of great importance to reduce the final coast, cultural conditions were analyzed to optimize biosurfactants production from Bacillus subtilis SPB1 strain. A high yield of biosurfactants was obtained from a culture of B. subtilis using carbohydrate substrate as a carbon source; among carbohydrates, glucose enhanced the best surfactin production. The optimum glucose concentration was 40 g/L. Higher amount of biosurfactants was obtained using 5 g/L of urea as organic nitrogen source and applying C/N ratio of 7 with ammonium chloride as inorganic nitrogen source. The highest amount of biosurfactants was recorded with the addition of 2% kerosene. Moreover, it was shown, using an automated full-controlled 2.6 L fermenter, that aeration of the medium, which affected strongly the growth regulated biosurfactants synthesis by the producing cell. So that, low or high aerations lead to a decrease of biosurfactants synthesis yields. It was found that when using dissolved oxygen saturation of the medium at 30%, biosurfactants production reached 4.92 g/L.

  10. Optimization of crude oil degradation by Dietzia cinnamea KA1, capable of biosurfactant production.

    Science.gov (United States)

    Kavynifard, Amirarsalan; Ebrahimipour, Gholamhossein; Ghasempour, Alireza

    2016-05-01

    The aim of this study was isolation and characterization of a crude oil degrader and biosurfactant-producing bacterium, along with optimization of conditions for crude oil degradation. Among 11 isolates, 5 were able to emulsify crude oil in Minimal Salt Medium (MSM) among which one isolate, named KA1, showed the highest potency for growth rate and biodegradation. The isolate was identified as Dietzia cinnamea KA1 using morphological and biochemical characteristics and 16S rRNA gene sequencing. The optimal conditions were 510 mM NaCl, pH 9.0, 35 °C, and minimal requirement of 46.5 mM NH4 Cl and 2.10 mM NaH2 PO4 . Gravimetric test and Gas chromatography-Mass spectroscopy technique (GC-MS) showed that Dietzia cinnamea KA1 was able to utilize and degrade 95.7% of the crude oil after 5 days, under the optimal conditions. The isolate was able to grow and produce biosurfactant when cultured in MSM supplemented with crude oil, glycerol or whey as the sole carbon sources, but bacterial growth was occurred using molasses with no biosurfactant production. This is the first report of biosurfactant production by D. cinnamea using crude oil, glycerol and whey and the first study to report a species of Dietzia degrading a wide range of hydrocarbons in a short time. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  11. Optimization of biosurfactant production by Bacillus brevis using response surface methodology

    Directory of Open Access Journals (Sweden)

    Foukia E. Mouafi

    2016-03-01

    Full Text Available The present study aims to evaluate and validate a statistical model for maximizing biosurfactant productivity by Bacillus brevis using response surface methodology. In this respect, twenty bacterial isolates were screened for biosurfactant production using hemolytic activity, oil spreading technique, and emulsification index (E24. The most potent biosurfactant-producing bacterium (B. brevis was used for construction of the statistical response surface model. The optimum conditions for biosurfactant production by B. brevis were: 33 °C incubation temperature at pH 8 for 10 days incubation period and 8.5 g/L glucose concentration as a sole carbon source. The produced biosurfactant (BS (73% exhibited foaming activity, thermal stability in the range 30–80 °C for 30 min., pH stability, from 4 to 9 and antimicrobial activity against (Escherichia coli. The BS gave a good potential application as an emulsifier.

  12. Investigation of antimicrobial activity and statistical optimization of Bacillus subtilis SPB1 biosurfactant production in solid-state fermentation.

    Science.gov (United States)

    Ghribi, Dhouha; Abdelkefi-Mesrati, Lobna; Mnif, Ines; Kammoun, Radhouan; Ayadi, Imen; Saadaoui, Imen; Maktouf, Sameh; Chaabouni-Ellouze, Semia

    2012-01-01

    During the last years, several applications of biosurfactants with medical purposes have been reported. Biosurfactants are considered relevant molecules for applications in combating many diseases. However, their use is currently extremely limited due to their high cost in relation to that of chemical surfactants. Use of inexpensive substrates can drastically decrease its production cost. Here, twelve solid substrates were screened for the production of Bacillus subtilis SPB1 biosurfactant and the maximum yield was found with millet. A Plackett-Burman design was then used to evaluate the effects of five variables (temperature, moisture, initial pH, inoculum age, and inoculum size). Statistical analyses showed that temperature, inoculum age, and moisture content had significantly positive effect on SPB1 biosurfactant production. Their values were further optimized using a central composite design and a response surface methodology. The optimal conditions of temperature, inoculum age, and moisture content obtained under the conditions of study were 37°C, 14 h, and 88%, respectively. The evaluation of the antimicrobial activity of this compound was carried out against 11 bacteria and 8 fungi. The results demonstrated that this biosurfactant exhibited an important antimicrobial activity against microorganisms with multidrug-resistant profiles. Its activity was very effective against Staphylococcus aureus, Staphylococcus xylosus, Enterococcus faecalis, Klebsiella pneumonia, and so forth.

  13. Investigation of Antimicrobial Activity and Statistical Optimization of Bacillus subtilis SPB1 Biosurfactant Production in Solid-State Fermentation

    Directory of Open Access Journals (Sweden)

    Dhouha Ghribi

    2012-01-01

    Full Text Available During the last years, several applications of biosurfactants with medical purposes have been reported. Biosurfactants are considered relevant molecules for applications in combating many diseases. However, their use is currently extremely limited due to their high cost in relation to that of chemical surfactants. Use of inexpensive substrates can drastically decrease its production cost. Here, twelve solid substrates were screened for the production of Bacillus subtilis SPB1 biosurfactant and the maximum yield was found with millet. A Plackett-Burman design was then used to evaluate the effects of five variables (temperature, moisture, initial pH, inoculum age, and inoculum size. Statistical analyses showed that temperature, inoculum age, and moisture content had significantly positive effect on SPB1 biosurfactant production. Their values were further optimized using a central composite design and a response surface methodology. The optimal conditions of temperature, inoculum age, and moisture content obtained under the conditions of study were 37°C, 14 h, and 88%, respectively. The evaluation of the antimicrobial activity of this compound was carried out against 11 bacteria and 8 fungi. The results demonstrated that this biosurfactant exhibited an important antimicrobial activity against microorganisms with multidrug-resistant profiles. Its activity was very effective against Staphylococcus aureus, Staphylococcus xylosus, Enterococcus faecalis, Klebsiella pneumonia, and so forth.

  14. Optimization of biosurfactant production from Vibrio sp. BSM-30 isolated in tropical waters

    Science.gov (United States)

    Su, Zengjian; Li, Min; Zhang, Yuxiu

    2017-01-01

    The strain BSM-30 (Vibrio sp.), isolated from Chinese tropical waters, could be a biosurfactant producing bacteria according with results obtained by the oil spreading method. The culture conditions for biosurfactant production were tested respectively such as inoculation (2%,6%,10%,14% as setting), shaking speed(120 r/min,150 r/min,180 r/min as setting), temperature (25°C,30°C,35°C as setting), pH (7,8,9 as setting), salinity (1.5%, 2.5%, 3.0%, 4.5%, 5.5% as setting), which results showed that the best culture conditions for BS production were 10% inoculation quantity, 180 r/min, 25°C, pH 8, and 3.5% salinity. The optimization of carbon sources (20g/ of glucose, 20g/L of starch, 20g/L of paraffin oil 20g/L of diesel, 20g/L of oil as setting) and nitrogen sources (6g/L of NaNO3,7.1g/L of KNO3,5.6g/L of NH4NO3,9.3g/L of (NH4)2SO4, 4.2g/L of CO(NH2)2 as setting) were also tested, which results showed that the best nitrogen source and carbon source were (NH4) 2SO4 and soluble starch.

  15. Optimization of biosurfactant production in soybean oil by rhodococcus rhodochrous and its utilization in remediation of cadmium-contaminated solution

    Science.gov (United States)

    Suryanti, Venty; Hastuti, Sri; Andriani, Dewi

    2016-02-01

    Biosurfactant production by Rhodococcus rhodochrous in soybean oil was developed, where the effect of medium composition and fermentation time were evaluated. The optimum condition for biosurfactant production was achieved when a medium containing 30 g/L TSB (tryptic soy broth) and 20% v/v soybean oil was used as media with 7 days of fermentation. Biosurfactant was identified as glycolipids type biosurfactant which had critical micelle concentration (CMC) value of 896 mg/L. The biosurfactant had oil in water emulsion type and was able to reduce the surface tension of palm oil about 52% which could stabilize the emulsion up to 12 days. The batch removal of cadmium metal ion by crude and partially purified biosurfactants have been examined from synthetic aqueous solution at pH 6. The results exhibited that the crude biosurfactant had a much better adsorption ability of Cd(II) than that of partially purified biosurfactant. However, it was found that there was no significant difference in the adsorption of Cd(II) with 5 and 10 minutes of contact time. The results indicated that the biosurfactant could be used in remediation of heavy metals from contaminated aqueous solution.

  16. Production and Biomedical Applications of Probiotic Biosurfactants.

    Science.gov (United States)

    Fariq, Anila; Saeed, Ayesha

    2016-04-01

    Biosurfactants have been widely used for environmental and industrial applications. However, their use in medical field is still limited. Probiotic biosurfactants possess an immense antimicrobial, anti-adhesive, antitumor, and antibiofilm potential. Moreover, they have an additional advantage over conventional microbial surfactants because probiotics are an integral part of normal human microflora and their biosurfactants are innocuous to human. So, they can be effectively exploited for medicinal use. Present review is aimed to discourse the production and biomedical applications of probiotic biosurfactants.

  17. Isolation of biosurfactant producers, optimization and properties of biosurfactant produced by Acinetobacter sp. from petroleum-contaminated soil.

    Science.gov (United States)

    Chen, J; Huang, P T; Zhang, K Y; Ding, F R

    2012-04-01

    To screen and identify biosurfactant producers from petroleum-contaminated soil; to use response surface methodology (RSM) for medium optimization to enhance biosurfactant production; and to study the properties of the newly obtained biosurfactant towards pH, temperature and salinity. We successfully isolated three biosurfactant producers from petroleum-contaminated soil and identified them through 16S rRNA sequence analysis, which exhibit the highest similarities to Acinetobacter beijerinckii (100%), Kocuria marina (99%) and Kineococcus marinus (99%), respectively. A quadratic response model was constructed through RSM designs, leading to a 57·5% increase of the growth-associated biosurfactant production by Acinetobacter sp. YC-X 2 with an optimized medium: beef extract 3·12 g l(-1) ; peptone 20·87 g l(-1) ; NaCl 1·04 g l(-1); and n-hexadecane 1·86 g l(-1). Biosurfactant produced by Acinetobacter sp. YC-X 2 retained its properties during exposure to a wide range of pH values (5-11), high temperatures (up to 121°C) and high salinities [up to 18% (w/v) Na(+) and Ca(2+) ], which was more sensitive to Ca(2+) than Na(+). Two novel biosurfactant producers were isolated from petroleum-contaminated soil. Biosurfactant from Acinetobacter sp. YC-X 2 has good properties to a wide range of pH, high temperature and high salinity, and its production was optimized successfully through RSM. The fact, an increasing demand of high-quality surfactants and the lack of cost-competitive bioprocesses of biosurfactants for commercial utilization, motivates researchers to develop cost-effective strategies for biosurfactant production through isolating new biosurfactant producers with special surface-active properties and optimizing their cultural conditions. Two novel biosurfactant producers in this study will widen our knowledge about this kind of micro-organism. This work is the first application of RSM designs for cultural optimization of biosurfactant produced by Acinetobacter

  18. Response surface optimization of biosurfactant produced by Pseudomonas aeruginosa MA01 isolated from spoiled apples.

    Science.gov (United States)

    Abbasi, Habib; Sharafi, Hakimeh; Alidost, Leila; Bodagh, Atefe; Zahiri, Hossein Shahbani; Noghabi, Kambiz Akbari

    2013-01-01

    A potent biosurfactant-producing bacterial strain isolated from spoiled apples was identified by 16S rRNA as Pseudomonas aeruginosa MA01. Compositional analysis revealed that the extracted biosurfactant was composed of high percentages of lipid (66%, w/w) and carbohydrate (32%, w/w). The surface tension of pure water decreased gradually with increasing biosurfactant concentration to 32.5 mN m(-1) with critical micelle concentration (CMC) value of 10.1 mg L(-1). The Fourier transform infrared spectrum of extracted biosurfactant confirmed the glycolipid nature of this natural product. Response surface methodology (RSM) was employed to optimize the biosynthesis medium for the production of MA01 biosurfactant. Nineteen carbon sources and 11 nitrogen sources were examined, with soybean oil and sodium nitrate being the most effective carbon and nitrogen sources on biosurfactant production, respectively. Among the organic nitrogen sources examined, yeast extract was necessary as a complementary nitrogen source for high production yield. Biosurfactant production at the optimum value of fermentation processing factor (15.68 g/L) was 29.5% higher than the biosurfactant concentration obtained before the RSM optimization (12.1 g/L). A central composite design algorithm was used to optimize the levels of key medium components, and it was concluded that two stages of optimization using RSM could increase biosurfactant production by 1.46 times, as compared to the values obtained before optimization.

  19. BIOSURFACTANTS PRODUCTION BY Pseudomonas aeruginosa USING SOYBEAN OIL AS SUBSTRATE

    Directory of Open Access Journals (Sweden)

    Venty Suryanti

    2010-06-01

    Full Text Available Optimization condition of the biosurfactants production by P. aeruginosa using soybean oil as substrate has been examined. The media containing 10% v/v of the soybean oil and 6 days of the fermentation time was the optimum condition for the biosurfactants production. The extraction technique using different solvent polarity (n-hexane, chloroform, ethyl acetate and buthanol, respectively was applied for the isolation of the biosurfactants. The biosurfactant was found in the extract chloroform of the crude biospasoy (biosurfactants obtained from soybean oil as substrate which then is called chlo-biospasoy. The chlo-biospasoy was identified as rhamnolipids which had oil in water (o/w emulsion type, had the CMC of 860 mg/L and could reduced the surface tension of the water from 72 mN/m to 52 mN/m. The chlo-biospasoy could be used as an emulsifier to form emulsion between water and hydrocarbon such as palm oil, benzene, premium or toluene with various stability. The results indicated that chlo-biospasoy could be used as an emulsifying and emulsion-stabilizing agent.     Keywords: Biosurfactants, P. aeruginosa, Soybean Oil, Emulsifier

  20. Detection of biosurfactants in Bacillus species: genes and products identification.

    Science.gov (United States)

    Płaza, G; Chojniak, J; Rudnicka, K; Paraszkiewicz, K; Bernat, P

    2015-10-01

    To screen environmental Bacillus strains for detection of genes encoding the enzymes involved in biosurfactant synthesis and to evaluate their products e.g. surfactin, iturin and fengycin. The taxonomic identification of isolated from the environment Bacillus strains was performed by Microgene ID Bacillus panel and GEN III Biolog system. The polymerase chain reaction (PCR) strategy for screening of genes in Bacillus strains was set up. Liquid chromatography-mass spectrometry (LC-MS/MS) method was used for the identification of lipopeptides (LPs). All studied strains exhibited the presence of srfAA gene and produced surfactin mostly as four homologues (C13 to C16). Moreover, in 2 strains (KP7, T'-1) simultaneous co-production of 3 biosurfactants: surfactin, iturin and fengycin was observed. Additionally, it was found out that isolate identified as Bacillus subtilis ssp. subtilis (KP7), beside LPs co-production, synthesizes surfactin with the efficiency much higher than other studied strains (40·2 mg l(-1) ) and with the yield ranging from 0·8 to 8·3 mg l(-1) . We showed that the combined methodology based on PCR and LC-MS/MS technique is an optimal tool for the detection of genes encoding enzymes involved in biosurfactant synthesis as well as their products, e.g. surfactin, iturin and fengycin. This approach improves the screening and the identification of environmental Bacillus co-producing biosurfactants-stimulating and facilitating the development of this area of science. The findings of this work will help to improve screening of biosurfactant producers. Discovery of novel biosurfactants and biosurfactants co-production ability has shed light on their new application fields and for the understanding of their interactions and properties. © 2015 The Society for Applied Microbiology.

  1. Biosurfactants production from cheese whey

    OpenAIRE

    Rodrigues, L. R.; Teixeira, J. A.

    2008-01-01

    Biosurfactants are molecules that exhibit pronounced surface and emulsifying activities, produced by a variety of microorganisms. A host of interesting features of biosurfactants, such as higher biodegradability, lower toxicity, and effectiveness at extremes of temperature, pH and salinity; have led to a wide range of potential applications in the fields of oil recovery, environmental bioremediation, food processing and medicine. In spite of the immense potential of...

  2. Production, Characterization, and Application of Bacillus licheniformis W16 Biosurfactant in Enhancing Oil Recovery.

    Science.gov (United States)

    Joshi, Sanket J; Al-Wahaibi, Yahya M; Al-Bahry, Saif N; Elshafie, Abdulkadir E; Al-Bemani, Ali S; Al-Bahri, Asma; Al-Mandhari, Musallam S

    2016-01-01

    The biosurfactant production by Bacillus licheniformis W16 and evaluation of biosurfactant based enhanced oil recovery (EOR) using core-flood under reservoir conditions were investigated. Previously reported nine different production media were screened for biosurfactant production, and two were further optimized with different carbon sources (glucose, sucrose, starch, cane molasses, or date molasses), as well as the strain was screened for biosurfactant production during the growth in different media. The biosurfactant reduced the surface tension and interfacial tension to 24.33 ± 0.57 mN m -1 and 2.47 ± 0.32 mN m -1 respectively within 72 h, at 40°C, and also altered the wettability of a hydrophobic surface by changing the contact angle from 55.67 ± 1.6 to 19.54°± 0.96°. The critical micelle dilution values of 4X were observed. The biosurfactants were characterized by different analytical techniques and identified as lipopeptide, similar to lichenysin-A. The biosurfactant was stable over wide range of extreme environmental conditions. The core flood experiments showed that the biosurfactant was able to enhance the oil recovery by 24-26% over residual oil saturation (S or ). The results highlight the potential application of lipopeptide biosurfactant in wettability alteration and microbial EOR processes.

  3. Production, Characterization and Application of Bacillus licheniformis W16 Biosurfactant in Enhancing Oil Recovery

    Directory of Open Access Journals (Sweden)

    Sanket J. Joshi

    2016-11-01

    Full Text Available The biosurfactant production by Bacillus licheniformis W16 and evaluation of biosurfactant based enhanced oil recovery using core-flood under reservoir conditions were investigated. Previously reported nine different production media were screened for biosurfactant production, and two were further optimized with different carbon sources (glucose, sucrose, starch, cane molasses or date molasses, as well as the strain was screened for biosurfactant production during the growth in different media. The biosurfactant reduced the surface tension and interfacial tension to 24.33+0.57mN m-1 and 2.47+0.32mN m-1 respectively within 72h, at 40 C, and also altered the wettability of a hydrophobic surface by changing the contact angle from 55.67°+1.6° to 19.54°+0.96°. The critical micelle dilution values of 4X were observed. The biosurfactants were characterized by different analytical techniques and identified as lipopeptide, similar to lichenysin-A. The biosurfactant was stable over wide range of extreme environmental conditions. The core flood experiments showed that the biosurfactant was able to enhance the oil recovery by 24-26% over residual oil saturation (Sor. The results highlight the potential application of lipopeptide biosurfactant in wettability alteration and microbial enhanced oil recovery processes.

  4. Optimization and characterization of biosurfactant from Streptomyces griseoplanus NRRL-ISP5009 (MS1).

    Science.gov (United States)

    Elkhawaga, M A

    2018-03-01

    This work aimed to study, isolate, characterize and stabilize the biosurfactant isolated from actinomycetes found in petroleum contaminated soil. Optimized production of the biosurfactant from Streptomyces griseoplanus NRRL-ISP5009, SM1 was obtained on day 6 at 30°C, pH 7, 150 rev min -1 , in glycerol yeast extract broth medium supplemented with cellulose, yeast extract and 1% NaCl. The stability of the biosurfactant produced was studied at different temperatures, pH and different concentrations of NaCl. The produced biosurfactant was extracted and purified. Streptomyces griseoplanus NRRL-ISP5009, SM1 isolated from oil contaminated soil produced a biosurfactant exhibiting emulsification activity. The produced biosurfactant is a mixture of carbohydrate, lipid and protein. It has promising characteristics, including a higher stability at alkaline pH than at acidic pH, a salinity of 1-3% and stable in the temperature range from 0 and 100°C. Also, the potential antimicrobial activity of the purified biosurfactant was recorded. The research was focused on the isolation of a novel source of biosurfactants that have great importance in the manufacture of food, detergent, pharmaceutical and cosmetics. © 2017 The Society for Applied Microbiology.

  5. Biosurfactant production by Bacillus subtilis B30 and its application in enhancing oil recovery.

    Science.gov (United States)

    Al-Wahaibi, Yahya; Joshi, Sanket; Al-Bahry, Saif; Elshafie, Abdulkadir; Al-Bemani, Ali; Shibulal, Biji

    2014-02-01

    The fermentative production of biosurfactants by Bacillus subtilis strain B30 and the evaluation of biosurfactant based enhanced oil recovery using core-flood were investigated. Different carbon sources (glucose, sucrose, starch, date molasses, cane molasses) were tested to determine the optimal biosurfactant production. The isolate B30 produced a biosurfactant that could reduce the surface tension and interfacial tension to 26.63±0.45 mN/m and 3.79±0.27 mN/m, respectively in less than 12h in both glucose or date molasses based media. A crude biosurfactant concentration of 0.3-0.5 g/l and critical micelle dilution (CMD) values of 1:8 were observed. The biosurfactants gave stable emulsions with wide range of hydrocarbons including light and heavy crude oil. The biosurfactants were partially purified and identified as a mixture of lipopeptides similar to surfactin, using high performance thin layer chromatography and Fourier transform infrared spectroscopy. The biosurfactants were stable over wide range of pH, salinity and temperatures. The crude biosurfactant preparation enhanced light oil recovery by 17-26% and heavy oil recovery by 31% in core-flood studies. The results are indicative of the potential of the strain for the development of ex situ microbial enhanced oil recovery processes using glucose or date molasses based minimal media. Copyright © 2013 Elsevier B.V. All rights reserved.

  6. BIOSURFACTANT PRODUCTION BY THERMOPHILIC DAIRY STREPTOCOCCI

    NARCIS (Netherlands)

    BUSSCHER, HJ; NEU, TR; VANDERMEI, HC

    Biosurfactant production of eight Streptococcus thermophilus strains, isolated from heat exchanger plates in the downstream side of the regenerator section of pasteurizers in the dairy industry has been measured using axisymmetric drop shape analysis by profile (ADSA-P). Strains were grown in M17

  7. Produção de biossurfactante por levedura Biosurfactants production by yeasts

    Directory of Open Access Journals (Sweden)

    Gizele Cardoso Fontes

    2008-01-01

    Full Text Available Biosurfactants are molecules extracellularly produced by bacteria, yeast and fungi that have significant interfacial activity properties. This review focuses on relevant parameters that influence biosurfactant production by yeasts. Many works have investigated the optimization of yeast biosurfactant production, mainly within the last decade, revealing that the potential of such microorganisms is not well explored in the industrial field. The main points to increase the process viability lays on the reduction of the production costs and enhancement of biosynthesis efficiency through optimization the culture conditions (carbon and nitrogen source, pH, aeration, speed agitation and the selection of inexpensive medium components.

  8. Parameters examination of a biosurfactant production at laboratory scale

    International Nuclear Information System (INIS)

    Rosero, Neira Gladys; Pimienta, Astrid Lorely; Dugarte, Fanny; Carvajal, Fredy Gonzalo

    2003-01-01

    This work presents the results obtained from the laboratory-scale experimentation for the optimization of production of rhamnolipid type biosurfactant in a batch process, through the calculation and analysis of yield parameters. Different carbon/nitrogen ratios were studied, for which the production rates of rhamnolipid under nitrogen limitation was defined. Bacterial growth yield parameters Y X/N and Y X/C , were also calculated

  9. Enhanced biosurfactant production through cloning of three genes and role of esterase in biosurfactant release

    Science.gov (United States)

    2011-01-01

    Background Biosurfactants have been reported to utilize a number of immiscible substrates and thereby facilitate the biodegradation of panoply of polyaromatic hydrocarbons. Olive oil is one such carbon source which has been explored by many researchers. However, studying the concomitant production of biosurfactant and esterase enzyme in the presence of olive oil in the Bacillus species and its recombinants is a relatively novel approach. Results Bacillus species isolated from endosulfan sprayed cashew plantation soil was cultivated on a number of hydrophobic substrates. Olive oil was found to be the best inducer of biosurfactant activity. The protein associated with the release of the biosurfactant was found to be an esterase. There was a twofold increase in the biosurfactant and esterase activities after the successful cloning of the biosurfactant genes from Bacillus subtilis SK320 into E.coli. Multiple sequence alignment showed regions of similarity and conserved sequences between biosurfactant and esterase genes, further confirming the symbiotic correlation between the two. Biosurfactants produced by Bacillus subtilis SK320 and recombinant strains BioS a, BioS b, BioS c were found to be effective emulsifiers, reducing the surface tension of water from 72 dynes/cm to as low as 30.7 dynes/cm. Conclusion The attributes of enhanced biosurfactant and esterase production by hyper-producing recombinant strains have many utilities from industrial viewpoint. This study for the first time has shown a possible association between biosurfactant production and esterase activity in any Bacillus species. Biosurfactant-esterase complex has been found to have powerful emulsification properties, which shows promising bioremediation, hydrocarbon biodegradation and pharmaceutical applications. PMID:21707984

  10. Biosurfactant production using mixed cultures under non-aseptic conditions

    International Nuclear Information System (INIS)

    Vipulanandan, C.; Ghurye, G.L.; Willson, R.C.

    1994-01-01

    The use of surfactants is of increasing interest for remediation of petroleum hydrocarbons in groundwater and soil. Surfactants increase the accessibility of adsorbed hydrocarbons and mobilize immiscible petroleum hydrocarbons for treatment. Biosurfactants have the advantage of biodegradability and non-toxicity over their synthetic counterparts, and can be produced from renewable sources. In this study the production of biosurfactant from molasses was investigated in continuously stirred batch reactors. The effects of substrate concentration, yeast extract and peptone on biomass accumulation and biosurfactant production were investigated. Biosurfactant production was quantified by surface tension reduction and critical micelle dilution (CMD). Biosurfactant production was directly correlated with biomass production, and was improved with the addition of yeast extract. Centrifugation of the whole broth reduced surface tension. The performance of the biosurfactant produced from molasses under non-aseptic condition is comparable to other published results

  11. Biosurfactants

    Science.gov (United States)

    Biosurfactants are surfactants whose common feature is biodegradability, which provides them with a major advantage over the majority of surfactants currently in the market. Biosurfactants are produced from a wide range of raw materials, and manufactured using chemical, enzymatic, microbial, and a c...

  12. Investigation of biosurfactant production by Bacillus pumilus 1529 and Bacillus subtilis WPI

    Directory of Open Access Journals (Sweden)

    shila khajavi shojaei

    2016-06-01

    Full Text Available Introduction: Biosurfactants are unique amphipathic molecules with extensive application in removing organic and metal contaminants. The purpose of this study was to investigate production of biosurfactant and determine optimal conditions to produce biosurfactant by Bacillus pumilus 1529 and Bacillus subtilis WPI. Materials and methods: In this study, effect of carbon source, temperature and incubation time on biosurfactant production was evaluated. Hemolytic activity, emulsification activity, oil spreading, drop collapse, cell hydrophobicity and measurement of surface tension were used to detect biosurfactant production. Then, according to the results, the optimal conditions for biosurfactant production by and Bacillus subtilis WPI was determined. Results: In this study, both bacteria were able to produce biosurfactant at an acceptable level. Glucose, kerosene, sugarcane molasses and phenanthrene used as a sole carbon source and energy for the mentioned bacteria. Bacillus subtilis WPI produced maximum biosurfactant in the medium containing kerosene and reduced surface tension of the medium to 33.1 mN/m after 156 hours of the cultivation at 37°C. Also, the highest surface tension reduction by Bacillus pumilus 1529 occurred in the medium containing sugarcane molasses and reduce the surface tension of culture medium after 156 hours at 37°C from 50.4 to 28.83 mN/m. Discussion and conclusion: Bacillus pumilus 1529 and Bacillus subtilis WPI had high potential in production of biosurfactant and degradation of petroleum hydrocarbons and Phenanthrene. Therefore, it could be said that these bacteria had a great potential for applications in bioremediation and other environmental process.

  13. Production of biosurfactants using substrates from renewable-resources

    Directory of Open Access Journals (Sweden)

    Suppasil Maneerat

    2005-05-01

    Full Text Available Surface-active compounds commonly used in industries are chemically synthesized. However, biosurfactants have been paid increasing attention to replace the synthetic surfactants owing to their advantages such as biodegradability and low toxicity. Nowadays, the use of biosurfactant has been limited due to the high production cost. Nevertheless, biosurfactants can be produced with high yield by some microorganisms, especially Pseudomonas sp. These microorganisms can use the various renewal resources, especially agroindustrial wastes, as the potential carbon sources. This leads to the greater possibility for economical biosurfactant production and reduced pollution caused by those wastes.

  14. Assessing Bacillus subtilis biosurfactant effects on the biodegradation of petroleum products.

    Science.gov (United States)

    Montagnolli, Renato Nallin; Lopes, Paulo Renato Matos; Bidoia, Ederio Dino

    2015-01-01

    Microbial pollutant removal capabilities can be determined and exploited to accomplish bioremediation of hydrocarbon-polluted environments. Thus, increasing knowledge on environmental behavior of different petroleum products can lead to better bioremediation strategies. Biodegradation can be enhanced by adding biosurfactants to hydrocarbon-degrading microorganism consortia. This work aimed to improve petroleum products biodegradation by using a biosurfactant produced by Bacillus subtilis. The produced biosurfactant was added to biodegradation assays containing crude oil, diesel, and kerosene. Biodegradation was monitored by a respirometric technique capable of evaluating CO₂ production in an aerobic simulated wastewater environment. The biosurfactant yielded optimal surface tension reduction (30.9 mN m(-1)) and emulsification results (46.90% with kerosene). Biodegradation successfully occurred and different profiles were observed for each substance. Precise mathematical modeling of biosurfactant effects on petroleum degradation profile was designed, hence allowing long-term kinetics prediction. Assays containing biosurfactant yielded a higher overall CO₂ output. Higher emulsification and an enhanced CO2 production dataset on assays containing biosurfactants was observed, especially in crude oil and kerosene.

  15. Production and Structural Characterization of Lactobacillus helveticus Derived Biosurfactant

    Science.gov (United States)

    Sharma, Deepansh; Saharan, Baljeet Singh; Chauhan, Nikhil; Bansal, Anshul; Procha, Suresh

    2014-01-01

    A probiotic strain of lactobacilli was isolated from traditional soft Churpi cheese of Yak milk and found positive for biosurfactant production. Lactobacilli reduced the surface tension of phosphate buffer saline (PBS) from 72.0 to 39.5 mNm−1 pH 7.2 and its critical micelle concentration (CMC) was found to be 2.5 mg mL−1. Low cost production of Lactobacilli derived biosurfactant was carried out at lab scale fermenter which yields 0.8 mg mL−1 biosurfactant. The biosurfactant was found least phytotoxic and cytotoxic as compared to the rhamnolipid and sodium dodecyl sulphate (SDS) at different concentration. Structural attributes of biosurfactant were determined by FTIR, NMR (1H and 13C), UPLC-MS, and fatty acid analysis by GCMS which confirmed the presence of glycolipid type of biosurfactant closely similar to xylolipids. Biosurfactant is mainly constituted by lipid and sugar fractions. The present study outcomes provide valuable information on structural characterization of the biosurfactant produced by L. helveticus MRTL91. These findings are encouraging for the application of Lactobacilli derived biosurfactant as nontoxic surface active agents in the emerging field of biomedical applications. PMID:25506070

  16. Production and characterization of biosurfactant from Pseudomonas ...

    African Journals Online (AJOL)

    Further characterization of biosurfactant using Fourier transform infrared spectroscopy (FTIR) revealed it as a rhamnolipid. Keywords: Mangrove ecosystems, Pseudomonas aeruginosa, biosurfactant, critical micelle concentration (CMC), FT-IR fourier transform infrared spectroscopy (FTIR). African Journal of Biotechnology, ...

  17. Dissolution Coupled Biodegradation of Pce by Inducing In-Situ Biosurfactant Production Under Anaerobic Conditions

    Science.gov (United States)

    Dominic, J.; Nambi, I. M.

    2013-12-01

    Biosurfactants have proven to enhance the bioavailability and thereby elevate the rate of degradation of Light Non Aqueous Phase Liquids (LNAPLs) such as crude oil and petroleum derivatives. In spite of their superior characteristics, use of these biomolecules for remediation of Dense Non Aqueous Phase Liquids (DNAPLs) such as chlorinated solvents is still not clearly understood. In this present study, we have investigated the fate of tetrachloroethylene (PCE) by inducing in-situ biosurfactants production, a sustainable option which hypothesizes increase in bioavailability of LNAPLs. In order to understand the effect of biosurfactants on dissolution and biodegradation under the inducement of in-situ biosurfactant production, batch experiments were conducted in pure liquid media. The individual influence of each process such as biosurfactant production, dissolution of PCE and biodegradation of PCE were studied separately for getting insights on the synergistic effect of each process on the fate of PCE. Finally the dissolution coupled biodegradation of non aqueous phase PCE was studied in conditions where biosurfactant production was induced by nitrate limitation. The effect of biosurfactants was differentiated by repeating the same experiments were the biosurfactant production was retarded. The overall effect of in-situ biosurfactant production process was evaluated by use of a mathematical model. The process of microbial growth, biosurfactant production, dissolution and biodegradation of PCE were translated as ordinary differential equations. The modelling exercise was mainly performed to get insight on the combined effects of various processes that determine the concentration of PCE in its aqueous and non-aqueous phases. Model simulated profiles of PCE with the kinetic coefficients evaluated earlier from individual experiments were compared with parameters fitted for observations in experiments with dissolution coupled biodegradation process using optimization

  18. Effects of Fe nanoparticles on bacterial growth and biosurfactant production

    Energy Technology Data Exchange (ETDEWEB)

    Liu Jia; Vipulanandan, Cumaraswamy, E-mail: cvipulanandan@uh.edu [University of Houston, Department of Civil and Environmental Engineering (United States); Cooper, Tim F. [University of Houston, Department of Biology and Biochemistry (United States); Vipulanandan, Geethanjali [University of Houston, Department of Biomedical Engineering (United States)

    2013-01-15

    Environmental conditions can have a major impact on bacterial growth and production of secondary products. In this study, the effect of different concentrations of Fe nanoparticles on the growth of Serratia sp. and on its production of a specific biosurfactant was investigated. The Fe nanoparticles were produced using the foam method, and the needle-shaped nanoparticles were about 30 nm in diameter. It was found that Fe nanoparticles can have either a positive or a negative impact on the bacterial growth and biosurfactant production, depending on their concentration. At 1 mg/L of Fe nanoparticle concentration the bacterial growth increased by 57 % and biosurfactant production increased by 63 %. When the Fe nanoparticle concentration was increased to 1 g/L, the bacterial growth decreased by 77 % and biosurfactant activity was undetectable. The biosurfactant itself was not directly affected by Fe nanoparticles over the range of concentrations studied, indicating that the observed changes in biosurfactant activity resulted indirectly from the effect of nanoparticles on the bacteria. These negative effects with nanoparticle exposures were temporary, demonstrated by the restoration of biosurfactant activity when the bacteria initially exposed to Fe nanoparticles were allowed to regrow in the absence of nanoparticles. Finally, the kinetics of bacterial growth and biosurfactant production were modeled. The model's predictions agreed with the experimental results.

  19. Effects of Fe nanoparticles on bacterial growth and biosurfactant production

    Science.gov (United States)

    Liu, Jia; Vipulanandan, Cumaraswamy; Cooper, Tim F.; Vipulanandan, Geethanjali

    2013-01-01

    Environmental conditions can have a major impact on bacterial growth and production of secondary products. In this study, the effect of different concentrations of Fe nanoparticles on the growth of Serratia sp. and on its production of a specific biosurfactant was investigated. The Fe nanoparticles were produced using the foam method, and the needle-shaped nanoparticles were about 30 nm in diameter. It was found that Fe nanoparticles can have either a positive or a negative impact on the bacterial growth and biosurfactant production, depending on their concentration. At 1 mg/L of Fe nanoparticle concentration the bacterial growth increased by 57 % and biosurfactant production increased by 63 %. When the Fe nanoparticle concentration was increased to 1 g/L, the bacterial growth decreased by 77 % and biosurfactant activity was undetectable. The biosurfactant itself was not directly affected by Fe nanoparticles over the range of concentrations studied, indicating that the observed changes in biosurfactant activity resulted indirectly from the effect of nanoparticles on the bacteria. These negative effects with nanoparticle exposures were temporary, demonstrated by the restoration of biosurfactant activity when the bacteria initially exposed to Fe nanoparticles were allowed to regrow in the absence of nanoparticles. Finally, the kinetics of bacterial growth and biosurfactant production were modeled. The model's predictions agreed with the experimental results.

  20. Effects of Fe nanoparticles on bacterial growth and biosurfactant production

    International Nuclear Information System (INIS)

    Liu Jia; Vipulanandan, Cumaraswamy; Cooper, Tim F.; Vipulanandan, Geethanjali

    2013-01-01

    Environmental conditions can have a major impact on bacterial growth and production of secondary products. In this study, the effect of different concentrations of Fe nanoparticles on the growth of Serratia sp. and on its production of a specific biosurfactant was investigated. The Fe nanoparticles were produced using the foam method, and the needle-shaped nanoparticles were about 30 nm in diameter. It was found that Fe nanoparticles can have either a positive or a negative impact on the bacterial growth and biosurfactant production, depending on their concentration. At 1 mg/L of Fe nanoparticle concentration the bacterial growth increased by 57 % and biosurfactant production increased by 63 %. When the Fe nanoparticle concentration was increased to 1 g/L, the bacterial growth decreased by 77 % and biosurfactant activity was undetectable. The biosurfactant itself was not directly affected by Fe nanoparticles over the range of concentrations studied, indicating that the observed changes in biosurfactant activity resulted indirectly from the effect of nanoparticles on the bacteria. These negative effects with nanoparticle exposures were temporary, demonstrated by the restoration of biosurfactant activity when the bacteria initially exposed to Fe nanoparticles were allowed to regrow in the absence of nanoparticles. Finally, the kinetics of bacterial growth and biosurfactant production were modeled. The model’s predictions agreed with the experimental results.

  1. Analytical modeling and numerical optimization of the biosurfactants production in solid-state fermentation by Aspergillus fumigatus - doi: 10.4025/actascitechnol.v36i1.17818

    Directory of Open Access Journals (Sweden)

    Gabriel Castiglioni

    2014-01-01

    Full Text Available This is an experimental, analytical and numerical study to optimize the biosurfactants production in solid-state fermentation of a medium containing rice straw and minced rice bran inoculated with Aspergillus fumigatus. The goal of this work was to analytically model the biosurfactants production in solid-state fermentation into a column fixed bed bioreactor. The Least-Squares Method was used to adjust the emulsification activity experimental values to a quadratic function semi-empirical model. Control variables were nutritional conditions, the fermentation time and the aeration. The mathematical model is validated against experimental results and then used to predict the maximum emulsification activity for different nutritional conditions and aerations. Based on the semi-empirical model the maximum emulsification activity with no additional hydrocarbon sources was 8.16 UE·g-1 for 112 hours. When diesel oil was used the predicted maximum emulsification activity was 8.10 UE·g-1 for 108 hours.

  2. Biosurfactant production by Pseudomonas strains isolated from floral nectar.

    Science.gov (United States)

    Ben Belgacem, Z; Bijttebier, S; Verreth, C; Voorspoels, S; Van de Voorde, I; Aerts, G; Willems, K A; Jacquemyn, H; Ruyters, S; Lievens, B

    2015-06-01

    To screen and identify biosurfactant-producing Pseudomonas strains isolated from floral nectar; to characterize the produced biosurfactants; and to investigate the effect of different carbon sources on biosurfactant production. Four of eight nectar Pseudomonas isolates were found to produce biosurfactants. Phylogenetic analysis based on three housekeeping genes (16S rRNA gene, rpoB and gyrB) classified the isolates into two groups, including one group closely related to Pseudomonas fluorescens and another group closely related to Pseudomonas fragi and Pseudomonas jessenii. Although our nectar pseudomonads were able to grow on a variety of water-soluble and water-immiscible carbon sources, surface active agents were only produced when using vegetable oil as sole carbon source, including olive oil, sunflower oil or waste frying sunflower oil. Structural characterization based on thin layer chromatography (TLC) and ultra high performance liquid chromatography-accurate mass mass spectrometry (UHPLC-amMS) revealed that biosurfactant activity was most probably due to the production of fatty acids (C16:0; C18:0; C18:1 and C18:2), and mono- and diglycerides thereof. Four biosurfactant-producing nectar pseudomonads were identified. The active compounds were identified as fatty acids (C16:0; C18:0; C18:1 and C18:2), and mono- and diglycerides thereof, produced by hydrolysis of triglycerides of the feedstock. Studies on biosurfactant-producing micro-organisms have mainly focused on microbes isolated from soils and aquatic environments. Here, for the first time, nectar environments were screened as a novel source for biosurfactant producers. As nectars represent harsh environments with high osmotic pressure and varying pH levels, further screening of nectar habitats for biosurfactant-producing microbes may lead to the discovery of novel biosurfactants with broad tolerance towards different environmental conditions. © 2015 The Society for Applied Microbiology.

  3. Production of biosurfactant by indigenous isolated bacteria in fermentation system

    Science.gov (United States)

    Fooladi, Tayebeh; Hamid, Aidil Bin Abd; Yusoff, Wan Mohtar Wan; Moazami, Nasrin; Shafiee, Zahra

    2013-11-01

    Bacillus pumilus 2IR is a soil isolate bacterium from an Iranian oil field that produces promising yield of biosurfactant in medium E. The production of biosurfactant by strain 2IR has been investigated using different carbon and nitrogen sources. The strain was able to grow and to produce surfactant, reducing the surface tension of the medium from 60mN/m to 31mN/m on glucose after 72 h of cultivation. The strain was able to produce the maximum amount of biosurfactant (0.72 g/l) when potassium nitrate and glucose used as a nitrogen and carbon sources respectively. Production of biosurfactant reaches to highest amount at a C/N ratio of 12.

  4. Candida lipolytica UCP0988 Biosurfactant: Potential as a Bioremediation Agent and in Formulating a Commercial Related Product

    Science.gov (United States)

    Santos, Danyelle K. F.; Resende, Ana H. M.; de Almeida, Darne G.; Soares da Silva, Rita de Cássia F.; Rufino, Raquel D.; Luna, Juliana M.; Banat, Ibrahim M.; Sarubbo, Leonie A.

    2017-01-01

    The aim of the present study was to investigate the potential application of the biosurfactant from Candida lipolytica grown in low-cost substrates, which has previously been produced and characterized under optimized conditions as an adjunct material to enhance the remediation processes of hydrophobic pollutants and heavy metals generated by the oil industry and propose the formulation of a safe and stable remediation agent. In tests carried out with seawater, the crude biosurfactant demonstrated 80% oil spreading efficiency. The dispersion rate was 50% for the biosurfactant at a concentration twice that of the CMC. The biosurfactant removed 70% of motor oil from contaminated cotton cloth in detergency tests. The crude biosurfactant also removed 30–40% of Cu and Pb from standard sand, while the isolated biosurfactant removed ~30% of the heavy metals. The conductivity of solutions containing Cd and Pb was sharply reduced after biosurfactants' addition. A product was prepared through adding 0.2% potassium sorbate as preservative and tested over 120 days. The formulated biosurfactant was analyzed for emulsification and surface tension under different pH values, temperatures, and salt concentrations and tested for toxicity against the fish Poecilia vivipara. The results showed that the formulation had no toxicity and did not cause significant changes in the tensoactive capacity of the biomolecule while maintaining activity demonstrating suitability for potential future commercial product formulation. PMID:28507538

  5. Candida lipolytica UCP0988 Biosurfactant: Potential as a Bioremediation Agent and in Formulating a Commercial Related Product

    Directory of Open Access Journals (Sweden)

    Leonie A. Sarubbo

    2017-05-01

    Full Text Available The aim of the present study was to investigate the potential application of the biosurfactant from Candida lipolytica grown in low-cost substrates, which has previously been produced and characterized under optimized conditions as an adjunct material to enhance the remediation processes of hydrophobic pollutants and heavy metals generated by the oil industry and propose the formulation of a safe and stable remediation agent. In tests carried out with seawater, the crude biosurfactant demonstrated 80% oil spreading efficiency. The dispersion rate was 50% for the biosurfactant at a concentration twice that of the CMC. The biosurfactant removed 70% of motor oil from contaminated cotton cloth in detergency tests. The crude biosurfactant also removed 30–40% of Cu and Pb from standard sand, while the isolated biosurfactant removed ~30% of the heavy metals. The conductivity of solutions containing Cd and Pb was sharply reduced after biosurfactants' addition. A product was prepared through adding 0.2% potassium sorbate as preservative and tested over 120 days. The formulated biosurfactant was analyzed for emulsification and surface tension under different pH values, temperatures, and salt concentrations and tested for toxicity against the fish Poecilia vivipara. The results showed that the formulation had no toxicity and did not cause significant changes in the tensoactive capacity of the biomolecule while maintaining activity demonstrating suitability for potential future commercial product formulation.

  6. Production of Biosurfactants by Actinomycetes Isolated from ...

    African Journals Online (AJOL)

    ASPIRE ONE D270

    biosurfactants are stable across temperature ranges and are not majorly affected by salt concentration; this property aids its potential ... for restoration of soil because the technology is ... technology is also dependent upon a microbial ability to ...

  7. Lactic Acid and Biosurfactants Production from Residual Cellulose Films.

    Science.gov (United States)

    Portilla Rivera, Oscar Manuel; Arzate Martínez, Guillermo; Jarquín Enríquez, Lorenzo; Vázquez Landaverde, Pedro Alberto; Domínguez González, José Manuel

    2015-11-01

    The increasing amounts of residual cellulose films generated as wastes all over the world represent a big scale problem for the meat industry regarding to environmental and economic issues. The use of residual cellulose films as a feedstock of glucose-containing solutions by acid hydrolysis and further fermentation into lactic acid and biosurfactants was evaluated as a method to diminish and revalorize these wastes. Under a treatment consisting in sulfuric acid 6% (v/v); reaction time 2 h; solid liquid ratio 9 g of film/100 mL of acid solution, and temperature 130 °C, 35 g/L of glucose and 49% of solubilized film was obtained. From five lactic acid strains, Lactobacillus plantarum was the most suitable for metabolizing the glucose generated. The process was scaled up under optimized conditions in a 2-L bioreactor, producing 3.4 g/L of biomass, 18 g/L of lactic acid, and 15 units of surface tension reduction of a buffer phosphate solution. Around 50% of the cellulose was degraded by the treatment applied, and the liqueurs generated were useful for an efficient production of lactic acid and biosurfactants using L. plantarum. Lactobacillus bacteria can efficiently utilize glucose from cellulose films hydrolysis without the need of clarification of the liqueurs.

  8. Kinetic study of biosurfactant production by Bacillus subtilis LAMI005 grown in clarified cashew apple juice.

    Science.gov (United States)

    de Oliveira, Darlane Wellen Freitas; França, Italo Waldimiro Lima; Félix, Anne Kamilly Nogueira; Martins, João Jeferson Lima; Giro, Maria Estela Aparecida; Melo, Vânia Maria M; Gonçalves, Luciana Rocha Barros

    2013-01-01

    In this work a low cost medium for the production of a biosurfactant by Bacillus subtilis LAMI005 and the kinetics of surfactin production considering the effect of initial substrate concentration were investigated. First, cashew apple juice supplementation for optimal production of biosurfactant by B. subtilis LAMI005 was studied. The medium formulated with clarified cashew apple juice and distilled water, supplemented with 1.0 g/L of (NH(4))(2)SO(4), proved to be the best among the nutrients evaluated. The crude biosurfactant had the ability to decrease the surface tension of water to 30 dyne/cm, with a critical micelle concentration (CMC) of 63.0 mg/L. Emulsification experiments indicated that this biosurfactant effectively emulsified kerosene (IE(24)=67%) and soybean oil (IE(24)=64%). Furthermore, the emulsion stability was always very high. It was shown by biochemical analysis, IR spectra, that there is no qualitative differences in the composition of the crude biosurfactant from a standard sample of surfactin from B. subtilis. Copyright © 2012 Elsevier B.V. All rights reserved.

  9. Production and applications of biosurfactant from Bacillus subtilis MUV4

    Directory of Open Access Journals (Sweden)

    Aran H-Kittikun

    2008-04-01

    Full Text Available Bacillus subtilis MUV4 produced biosurfactant in shake-flask culture (200 rpm at 30oC with modified Mckeen medium containing 1% glucose as a carbon source, 1% monosodium glutamate and 0.3% yeast extract as nitrogen sources. The supernatant of B. subtilis MUV4 reduced the surface tension of the medium from 53.50 mN/m to 33.50 mN/m after 48 h of cultivation. The yield of crude biosurfactant from B. subtilis MUV4 after precipitating the supernatant with 6N HCl was 0.652 g/L. Growth kinetics studies showed the specific growth rate (μ of 0.14 h-1, yield of biomass to substrate (Yx/s of 0.713, yield of product to substrate (Yp/s of 0.072 and yield of product to biomass (Yp/x of 0.101. Moreover, B. subtilis MUV4 produced 0.30 g/L crude biosurfactant after 96 h of cultivation in the fermentor with agitation rate of 200 rpm without aeration and uncontrolled pH condition. The crude biosurfactant was dissolved in methanol and dried by vacuum evaporator (crude methanol. The supernatant, the crude biosurfactant and the crude methanol retained the biosurfactant activity over the pH range of 1-6, 7-10 and 4-10, respectively and the emulsion stability at 24 h (E24 at pH 7 were 66.67%, 33.33% and 33.33%, respectively. The supernatant and the crude biosurfactant showed surface tension activity at 4oC, room temperature (30±2oC and 50oC after incubation for 5 h. However, only crude methanol still retained surface tension activity after 100oC for 5 h. The surface tension activity of the supernatant and the crude biosurfactant was stable in 3-10% (w/v NaCl while crude methanol showed stability in 3-20% (w/v NaCl. However, all samples lost emulsion stability when NaCl concentration was higher than 5% (w/v. With sand pack column technique, crude methanol enhanced the recovery of crude oil and kerosene oil by 41.85% and 75.00%, respectively. In hydrocarbon degradation application study, the crude biosurfactant was added to the culture medium containing 0.3% crude oil

  10. Utilization of oleo-chemical industry by-products for biosurfactant production

    Science.gov (United States)

    2013-01-01

    Biosurfactants are the surface active compounds produced by micro-organisms. The eco-friendly and biodegradable nature of biosurfactants makes their usage more advantageous over chemical surfactants. Biosurfactants encompass the properties of dropping surface tension, stabilizing emulsions, promoting foaming and are usually non- toxic and biodegradable. Biosurfactants offer advantages over their synthetic counterparts in many applications ranging from environmental, food, and biomedical, cosmetic and pharmaceutical industries. The important environmental applications of biosurfactants include bioremediation and dispersion of oil spills, enhanced oil recovery and transfer of crude oil. The emphasis of present review shall be with reference to the commercial production, current developments and future perspectives of a variety of approaches of biosurfactant production from the micro-organisms isolated from various oil- contaminated sites and from the by-products of oleo-chemical industry wastes/ by-products viz. used edible oil, industrial residues, acid oil, deodorizer distillate, soap-stock etc. PMID:24262384

  11. Production of lipopeptide biosurfactants by Bacillus atrophaeus 5-2a and their potential use in microbial enhanced oil recovery.

    Science.gov (United States)

    Zhang, Junhui; Xue, Quanhong; Gao, Hui; Lai, Hangxian; Wang, Ping

    2016-10-03

    Lipopeptides are known as promising microbial surfactants and have been successfully used in enhancing oil recovery in extreme environmental conditions. A biosurfactant-producing strain, Bacillus atrophaeus 5-2a, was recently isolated from an oil-contaminated soil in the Ansai oilfield, Northwest China. In this study, we evaluated the crude oil removal efficiency of lipopeptide biosurfactants produced by B. atrophaeus 5-2a and their feasibility for use in microbial enhanced oil recovery. The production of biosurfactants by B. atrophaeus 5-2a was tested in culture media containing eight carbon sources and nitrogen sources. The production of a crude biosurfactant was 0.77 g L -1 and its surface tension was 26.52 ± 0.057 mN m -1 in a basal medium containing brown sugar (carbon source) and urea (nitrogen source). The biosurfactants produced by the strain 5-2a demonstrated excellent oil spreading activity and created a stable emulsion with paraffin oil. The stability of the biosurfactants was assessed under a wide range of environmental conditions, including temperature (up to 120 °C), pH (2-13), and salinity (0-50 %, w/v). The biosurfactants were found to retain surface-active properties under the extreme conditions. Additionally, the biosurfactants were successful in a test to simulate microbial enhanced oil recovery, removing 90.0 and 93.9 % of crude oil adsorbed on sand and filter paper, respectively. Fourier transform infrared spectroscopy showed that the biosurfactants were a mixture of lipopeptides, which are powerful biosurfactants commonly produced by Bacillus species. The study highlights the usefulness of optimization of carbon and nitrogen sources and their effects on the biosurfactants production and further emphasizes on the potential of lipopeptide biosurfactants produced by B. atrophaeus 5-2a for crude oil removal. The favorable properties of the lipopeptide biosurfactants make them good candidates for application in the bioremediation of oil

  12. Production and antimicrobial property of glycolipid biosurfactants

    Science.gov (United States)

    Microbial glycolipids such as rhamnolipid (RL) and sophorolipid (SL) are an important class of biosurfactants with excellent surface tension-lowering activity. Besides their surfactant- and environment-friendly properties, however, additional value-added property such as bacteriocidal activity is n...

  13. Current status in biotechnological production and applications of glycolipid biosurfactants.

    Science.gov (United States)

    Paulino, Bruno Nicolau; Pessôa, Marina Gabriel; Mano, Mario Cezar Rodrigues; Molina, Gustavo; Neri-Numa, Iramaia Angélica; Pastore, Glaucia Maria

    2016-12-01

    Biosurfactants are natural compounds with surface activity and emulsifying properties produced by several types of microorganisms and have been considered an interesting alternative to synthetic surfactants. Glycolipids are promising biosurfactants, due to low toxicity, biodegradability, and chemical stability in different conditions and also because they have many biological activities, allowing wide applications in different fields. In this review, we addressed general information about families of glycolipids, rhamnolipids, sophorolipids, mannosylerythritol lipids, and trehalose lipids, describing their chemical and surface characteristics, recent studies using alternative substrates, and new strategies to improve of production, beyond their specificities. We focus in providing recent developments and trends in biotechnological process and medical and industrial applications.

  14. Isolation, Fermentation Optimization and Performance Studies of a Novel Biosurfactant Producing Strain Bacillus amyloliquefaciens

    OpenAIRE

    Zhang, W.; Zhang, X.; Cui, H.

    2015-01-01

    In this research, biosurfactant-producing bacteria were isolated from the outlet sludge of a canteen and one promising strain was identified through 16S rDNA sequence as Bacillus amyloliquefaciens. This strain can utilize water-soluble carbon source and the FT-IR analysis indicated the biosurfactant was probably glycolipids. Further factors (fermentation time, temperature, carbon source, nitrogen source, ion concentration) affecting the biosurfactant production were determined. The optimum fe...

  15. Production of microbial glycolipid biosurfactants and their antimicrobial activity

    Science.gov (United States)

    Microbial glycolipids produced by bacteria or yeast as secondary metabolites, such as sophorolipids (SLs), rhamnolipids (RLs) and mannosylerythritol lipids (MELs) are “green” biosurfactants desirable in a bioeconomy. High cost of production is a major hurdle toward widespread commercial use of bios...

  16. Diesel degradation and biosurfactant production by Gram-positive ...

    African Journals Online (AJOL)

    The ability of Gram-positive bacteria to degrade diesel increased in a comparable trend as its biosurfactant production increased. The E24 index was highest at 87.6% for isolate D9. Isolates D2, D9 and D10, were identified as Paenibacillus sp. whilst isolate DJLB was found to belong to Stenotrophomonas sp. This study ...

  17. Production of a rhamnolipid-type biosurfactant by Pseudomonas ...

    African Journals Online (AJOL)

    The work herewith investigated the effect of the culture medium composition on rhamnolipid production by Pseudomonas aeruginosa LBM10, previously isolated from an estuarine environment in Southern Brazil. Experimental design and surface response methodology were used in order to improve biosurfactant ...

  18. Kinetic study and modeling of biosurfactant production using Bacillus sp.

    Directory of Open Access Journals (Sweden)

    Hesty Heryani

    2017-05-01

    Conclusions: For further development and industrial applications, the modified Gompertz equation is proposed to predict the cell mass and biosurfactant production as a goodness of fit was obtained with this model. The modified Gompertz equation was also extended to enable the excellent prediction of the surface tension.

  19. Indigenous production of biosurfactant and degradation of crude oil

    Directory of Open Access Journals (Sweden)

    Hamid Rashedi

    2015-04-01

    Full Text Available The present study investigated the isolation and identification of biosurfactant producing bacteria from Iranian oil wells. The biosurfactant production of bacteria isolates was evaluated and confirmed using hemolysis and emulsification tests. The biodegradation of crude oil was studied using GC and HPLC analysis. A total of 45 strains have been isolated. These strains showed less than a 40 mN m-1 reduction in surface tension. The effects of different pH (4.2-9.2, salinity concentrations (1%-15%, and temperatures (25-50 in biosurfactant production of isolated strains were evaluated. One of the strains (Bacillus sp. NO.4 showed a high salt tolerance and a successful production of biosurfactant in a vast pH range. Its maximum biomass production (about 3.1 g L-1 dry weight was achieved after 60 hours of growth. The surface tension of the culture broth dropped rapidly after inoculation and reached its lowest value (36 mN m-1 during the exponential phase after about 36-48 hours of growth. The study of the GC graphs showed that higher aliphatic reduction occurred in fractions with C14 to C24 hydrocarbons. The depicted results of the HPLC graphs indicated a 100% degradation of chrysene and fluorine. In this study, we demonstrated the useful capacities of the isolates in removing oil pollutants and their application in MEOR in vitro.

  20. Identification of potential local isolated for biosurfactant production

    Science.gov (United States)

    Shafiei, Zahra; Yusoff, Wan Mohtar Wan; Hamid, Aidil Abdul; Moazami, Nasrin; Hamzah, Ainon; Fooladi, Taybeh

    2013-11-01

    Biosurfactant are amphiphilic molecule that have received increasing attention in recent years because of their role in the growth of microorganisms on water-insoluble hydrophobic materials such as hydrocarbons as well as their commercial potential in the cosmetics, food, oil recovery and agricultural industries. In this study a potential biosurfactant producing strain was isolated from several soil samples of Terengganu oil refinery, Malaysia and selected during preliminary screening using hemolytic activity, oil spreading and drop collapsed technique. Isolates with at least more than one positive response to these three methods were subjected to complementary screening by measuring surface tension reduction as well as emulsification capacity. The biosurfactant produced by isolated 5M was able to reduced surface tension of culture medium from 60 mN/m to30mN/m. The biochemical and morphological characterization, 16SrRNA gene sequencing showed that the isolated 5M belongs to bacillus groups. The maximum production of biosurfactant by Bacillus 5M was observed after 48 h of incubation.

  1. Optimization of liquid-liquid extraction of biosurfactants from corn steep liquor.

    Science.gov (United States)

    Vecino, X; Barbosa-Pereira, L; Devesa-Rey, R; Cruz, J M; Moldes, A B

    2015-09-01

    In this work, the optimization of the operational conditions for the chloroform-based extraction of surface-active compounds from corn steep liquor (CSL) was carried out and the nutritional properties of the remnant aqueous phase (CSL-less biosurfactant) was evaluated as microbial fermentation medium. The optimal conditions to obtain biosurfactants from CSL were as follows: chloroform/CSL ratio 2 (v/v), 56 °C at extraction times >30 min. At the optima conditions, 100 % of biosurfactant extract can be obtained from CSL, obtaining 12.0 ± 0.5 g of biosurfactant extract/Kg of CSL. The critical micelle concentration (CMC) of the biosurfactant extract was 399.4 mg L(-1). This value is similar to the CMC of cetrimonium bromide (CTAB), a cationic surfactant used in the formulation of nanoparticles. The extraction of biosurfactant can be also carried out at room temperature although in this case, the extraction yield decreased about 15 %. The extraction of surface-active compounds from agroindustrial streams can suppose important advances for the bio-based surfactants industry. Biosurfactants obtained in this work are not only more eco-friendly than chemical detergents but also can be cost competitive with its chemical counterparts. Furthermore, after the extraction of surface-active compounds, CSL-less biosurfactant was found to be suitable as nutritional supplement for lactic acid bacteria, maintaining its nutritional properties in comparison with regular CSL.

  2. Production and characterization of novel self-assembling biosurfactants from Aspergillus flavus.

    Science.gov (United States)

    Ishaq, U; Akram, M S; Iqbal, Z; Rafiq, M; Akrem, A; Nadeem, M; Shafi, F; Shafiq, Z; Mahmood, S; Baig, M A

    2015-10-01

    This work was conducted to produce, purify and characterize biosurfactants from Aspergillus flavus AF612 isolated from citrus fruit. Biosurfactant named 'Uzmaq' was isolated from A. flavus AF612. The chemical characterization of the biosurfactant was conducted. Biosurfactant Uzmaq produced by A. flavus, was composed of methoxy phenyl oxime glycosides. Two molecular forms of the biosurfactant, Uzmaq-A and Uzmaq-B were isolated. Biological properties (antifungal activity) were evaluated. The fractions of the biosurfactant were isolated and their surface properties were analysed. Uzmaq-A and Uzmaq-B had critical micelle concentration (CMC) around 170 and 80 mg l(-1) , and lowered surface tension of water up to 20 and 25 m Nm(-1) respectively. The biosurfactants were stable at pH 3-12 and temperature up to 80°C. Growth and biosurfactant production kinetics were also analysed. Novel biosurfactant Uzmaq was produced from A. flavus, which was composed of methoxy phenyl oxime glycosides. The surface activity of Uzmaq was better than the maximum values of synthetic chemical surfactants. The biosurfactant showed antifungal activity and self-assembling properties. Aspergillus flavus AF612 can be used for commercial production of Uzmaq that may be employed for controlled drug release applications and bioremediation. © 2015 The Society for Applied Microbiology.

  3. Utilization of palm oil decanter cake as a novel substrate for biosurfactant production from a new and promising strain of Ochrobactrum anthropi 2/3.

    Science.gov (United States)

    Noparat, Pongsak; Maneerat, Suppasil; Saimmai, Atipan

    2014-03-01

    A biosurfactant-producing bacterium, isolate 2/3, was isolated from mangrove sediment in the south of Thailand. It was evaluated as a potential biosurfactant producer. The highest biosurfactant production (4.52 g/l) was obtained when the cells were grown on a minimal salt medium containing 25 % (v/v) palm oil decanter cake and 1 % (w/v) commercial monosodium glutamate as carbon and nitrogen sources, respectively. After microbial cultivation at 30 °C in an optimized medium for 96 h, the biosurfactant produced was found to reduce the surface tension of pure water to 25.0 mN/m with critical micelle concentrations of 8.0 mg/l. The stability of the biosurfactant at different salinities, pH and temperature and also its emulsifying activity was investigated. It is an effective surfactant at very low concentrations over a wide range of temperatures, pH and salt concentrations. The biosurfactant obtained was confirmed as a glycolipid type biosurfactant by using a biochemical test, fourier-transform infrared spectroscopy, MNR and mass spectrometry. The crude biosurfactant showed a broad spectrum of antimicrobial activity and also had the ability to emulsify oil and enhance polyaromatic hydrocarbons solubility.

  4. Production and characterization of biosurfactant from marine Streptomyces species B3.

    Science.gov (United States)

    Khopade, Abhijit; Ren, Biao; Liu, Xiang-Yang; Mahadik, Kakasaheb; Zhang, Lixin; Kokare, Chandrakant

    2012-02-01

    The present study demonstrates the production and properties of a biosurfactant isolated from marine Streptomyces species B3. The production of the biosurfactant was found to be higher in medium containing sucrose and lower in the medium containing glycerol. Yeast extract was the best nitrogen source for the production of the biosurfactant. The isolated biosurfactant reduced the surface tension of water to 29 mN/m. The purified biosurfactant was shown critical micelle concentrations of 110 mg/l. The emulsifying activity and stability of the biosurfactant was investigated at different salinities, pH, and temperature. The biosurfactant was effective at very low concentrations over a wide range of temperature, pH, and salt concentration. The purified biosurfactant was shown strong antimicrobial activity. The biosurfactant was produced from the marine Streptomyces sp. using non-hydrocarbon substrates such as sucrose that was readily available and not required extensive purification procedure. Streptomyces species B3 can be used for microbially enhanced oil recovery process. Copyright © 2011 Elsevier Inc. All rights reserved.

  5. Production, characterization, and antifungal activity of a biosurfactant produced by Rhodotorula babjevae YS3.

    Science.gov (United States)

    Sen, Suparna; Borah, Siddhartha Narayan; Bora, Arijit; Deka, Suresh

    2017-05-30

    Sophorolipids are one of the most promising glycolipid biosurfactants and have been successfully employed in bioremediation and various other industrial sectors. They have also been described to exhibit antimicrobial activity against different bacterial species. Nevertheless, previous literature pertaining to the antifungal activity of sophorolipids are limited indicating the need for further research to explore novel strains with wide antimicrobial activity. A novel yeast strain, Rhodotorula babjevae YS3, was recently isolated from an agricultural field in Assam, Northeast India. This study was primarily emphasized at the characterization and subsequent evaluation of antifungal activity of the sophorolipid biosurfactant produced by R. babjevae YS3. The growth kinetics and biosurfactant production by R. babjevae YS3 was evaluated by cultivation in Bushnell-Haas medium containing glucose (10% w/v) as the sole carbon source. A reduction in the surface tension of the culture medium from 70 to 32.6 mN/m was observed after 24 h. The yield of crude biosurfactant was recorded to be 19.0 g/l which might further increase after optimization of the growth parameters. The biosurfactant was characterized to be a heterogeneous sophorolipid (SL) with both lactonic and acidic forms after TLC, FTIR and LC-MS analyses. The SL exhibited excellent oil spreading and emulsifying activity against crude oil at 38.46 mm 2 and 100% respectively. The CMC was observed to be 130 mg/l. The stability of the SL was evaluated over a wide range of pH (2-10), salinity (2-10% NaCl) and temperature (at 120 °C for time intervals of 30 up to 120 min). The SL was found to retain surface-active properties under the extreme conditions. Additionally, the SL exhibited promising antifungal activity against a considerably broad group of pathogenic fungi viz. Colletotrichum gloeosporioides, Fusarium verticilliodes, Fusarium oxysporum f. sp. pisi, Corynespora cassiicola, and Trichophyton rubrum. The

  6. SCREENING OF BIOSURFACTANT PRODUCTION BY BACILLUS SP ISOLATED FROM COASTAL REGION IN CUDDALORE TAMILNADU

    OpenAIRE

    Bhuvaneswari. M*and P. Sivagurunathan

    2016-01-01

    Marine microorganisms produce extracellular or membrane associated surface-active compounds (bio surfactants). Biosurfactant are organic compounds belonging to various classes including glycolipids, lipopeptides, fatty acids, phospholipids that reduce the interfacial tension between immiscible liquids.This study deals with production and characterization of biosurfactant from Bacillus sp. The efficiency of Bacillus spstrain isolated from a marine sediments soil sample from coastal region -Cud...

  7. Oxygen-controlled Biosurfactant Production in a Bench Scale Bioreactor

    Science.gov (United States)

    de Kronemberger, Frederico Araujo; Anna, Lidia Maria Melo Santa; Fernandes, Ana Carolina Loureiro Brito; de Menezes, Reginaldo Ramos; Borges, Cristiano Piacsek; Freire, Denise Maria Guimarães

    Rhamnolipids have been pointed out as promising biosurfactants. The most studied microorganisms for the aerobic production of these molecules are the bacteria of the genus Pseudomonas. The aim of this work was to produce a rhamnolipid-type biosurfactant in a bench-scale bioreactor by one strain of Pseudomonas aeruginosa isolated from oil environments. To study the microorganism growth and production dependency on oxygen, a nondispersive oxygenation device was developed, and a programmable logic controller (PLC) was used to set the dissolved oxygen (DO) concentration. Using the data stored in a computer and the predetermined characteristics of the oxygenation device, it was possible to evaluate the oxygen uptake rate (OUR) and the specific OUR (SOUR) of this microorganism. These rates, obtained for some different DO concentrations, were then compared to the bacterial growth, to the carbon source consumption, and to the rhamnolipid and other virulence factors production. The SOUR presented an initial value of about 60.0 mg02/gdw h. Then, when the exponential growth phase begins, there is a rise in this rate. After that, the SOUR reduces to about 20.0 mg02/gdw h. The carbon source consumption is linear during the whole process.

  8. Biosurfactant production by yeasts isolated from hydrocarbon polluted environments.

    Science.gov (United States)

    Kaur, Kamalpreet; Sangwan, Seema; Kaur, Harpreet

    2017-11-03

    Thirty-two yeast isolates were retrieved from four soil samples collected from hydrocarbon-polluted locations of Hisar, Haryana, using enrichment culture technique with 1% (v/v) diesel as carbon source. Total nine isolates showing blood agar haemolysis were screened further for biosurfactant production. Yeast isolate, YK32, gave highest 8.4-cm oil displacement which was found to be significantly higher as compared to positive control, 0.2% (w/v) SDS (6.6 cm), followed by 6.2 and 6.0 cm by isolates YK20 and YK21, respectively. Maximum emulsification index was obtained in case of isolates YK20 and YK21 measuring 53.8%, after 6 days of incubation utilizing glucose as carbon source, whereas isolate YK32 was found to be reducing surface tension up to 93 dynes/cm and presented 99.6% degree of hydrophobicity. Olive oil has supported maximum surface tension reduction in isolates YK32 and YK21 equivalent to 53 and 48 dynes/cm and gave 88.3 and 88.5% degree of hydrophobicity, respectively. Diesel was not preferred as carbon source by most of the isolates except YK28 which generated 5.5-cm oil displacement, 25% emulsification index, reduced surface tension to the level of 38 dynes/cm and presented 89% degree of hydrophobicity. Conclusively, isolates YK20, YK21, YK22 and YK32 were marked as promising biosurfactant producers and were subjected to identification. Based on microscopic examination and biochemical peculiarities, isolates YK21 and YK22 might be identified as Candida spp., whereas, isolates YK20 and YK32 might be identified as Saccharomycopsis spp. and Brettanomyces spp., respectively. Interestingly it is the first report indicating Saccharomycopsis spp. and Brettanomyces spp. as a potential biosurfactant producer.

  9. Biosurfactant Production by Pseudomonas aeruginosa and Burkholderia gladioli Isolated from Mangrove Sediments Using Alternative Substrates

    Directory of Open Access Journals (Sweden)

    Karla Maria Catter

    2016-10-01

    Full Text Available Biosurfactants are surface-active agents produced by a variety of microorganisms. To make biosurfactant production economically feasible, several alternative carbon sources have been proposed. This study describes biosurfactant production by strains of Pseudomonas aeruginosa and Burkholderia gladioli isolated from mangrove sediments in Northeastern Brazil and cultured in mineral media enriched with waste cooking oil. The biosurfactants were tested for drop collapse, emulsion formation and stability and surface tension. P. aeruginosa performed better both at lowering the surface tension (from 69 to 28 mN/m and at forming stable emulsions (approximately 80% at 48 hours of culture. The strains tested in this study were found to be efficient biosurfactant producers when cultured on substrates enriched with vegetable oil. DOI: http://dx.doi.org/10.17807/orbital.v8i5.771

  10. Biosurfactant production by Pseudomonas fluorescens growing on molasses and its application in phenol degradation

    Science.gov (United States)

    Suryantia, Venty; Marliyana, Soerya Dewi; Wulandari, Astri

    2015-12-01

    A molasses based medium for the biosurfactant production by Pseudomonas fluorescens was developed, where the effect of pre-treated of molasses and medium composition were evaluated. Biosurfactant production was followed by measuring optical density (OD), surface tension and emulsifying index (E24) over 12 days of fermentation. The optimum condition for the biosurfactant production was obtained when a medium containing of 8 g/L nutrient broth, 5 g/L NaCl, 1 g/L NH4NO3 and 5% v/v pre-treated molasses with centrifugation was used as media with 3 days of fermentation. The biosurfactant was identified as a rhamnolipid type biosurfactant which had critical micelle concentration (CMC) value of 801 mg/L and was able to reduce the surface tension of the water from 80 mN/m to 51 mN/m. The biosurfactants had water in oil (w/o) emulsion type. Biosurfactant was able to emulsify various hydrocarbons, which were able to decrase the interfacial tension about 50-75% when benzyl chloride, anisaldehyde and palm oil were used as immiscible compounds. The biosurfactant exhibited the E24 value of about 50% and the stable emulsion was reached up to 30 days when lubricant was used as an immiscible compound. Up to 68% of phenol was degraded in the presence of biosurfactant within 15 days, whereas only 56% of phenol was degraded in the absence of biosurfactant. Overall, the results exhibited that molasses are recommended for the rhamnolipids production which possessed good surface-active properties and had potential application in the enhancement of phenol degradation.

  11. Simultaneous production of detergent stable keratinolytic protease, amylase and biosurfactant by Bacillus subtilis PF1 using agro industrial waste.

    Science.gov (United States)

    Bhange, Khushboo; Chaturvedi, Venkatesh; Bhatt, Renu

    2016-06-01

    The present study is an attempt to optimize simultaneous production of keratinolytic protease, amylase and biosurfactant from feather meal, potato peel and rape seed cake in a single media by response surface methodology to evaluate their biochemical properties for detergent additive. The optimization was carried out using 20 run, 3 factor and 5-level of central composite design on design expert software which resulted in a 1.2, 0.84 and 2.28 fold increase in protease, amylase and biosurfactant production. The proteolytic activity was found to be optimum at pH 9.0 and 60 °C while optimum amylolytic activity was recorded at pH 6.0 and 70 °C respectively. Both enzymes were found to be stable in the presence of organic solvents, ionic and commercial detergent and oxidizing agents. The biosurfactant was extracted with chloroform and was found to be stable at varying pH and temperature; however a reduction in the activity was observed at temperature higher than 70 °C. The isolated enzymes and biosurfactants may find applications in the effective removal of stains.

  12. Utilization of sludge palm oil as a novel substrate for biosurfactant production.

    Science.gov (United States)

    Wan Nawawi, Wan Mohd Fazli; Jamal, Parveen; Alam, Md Zahangir

    2010-12-01

    This paper introduces sludge palm oil (SPO) as a novel substrate for biosurfactant production by liquid state fermentation. Potential strains of microorganism were isolated from various hydrocarbon-based sources at palm oil mill and screened for biosurfactant production with the help of drop collapse method and surface tension activity. Out of 22 isolates of microorganism, the strain S02 showed the highest bacterial growth with a surface tension of 36.2 mN/m and was therefore, selected as a potential biosurfactant producing microorganism. Plackett-Burman experimental design was employed to determine the important nutritional requirement for biosurfactant production by the selected strain under controlled conditions. Six out of 11 factors of the production medium were found to significantly affect the biosurfactant production. K(2)HPO(4) had a direct proportional correlation with the biosurfactant production while sucrose, glucose, FeSO(4), MgSO(4), and NaNO(3) showed inversely proportional relationship with biosurfactant production in the selected experimental range. 2010 Elsevier Ltd. All rights reserved.

  13. Production and characterization of biosurfactant produced by a novel Pseudomonas sp. 2B.

    Science.gov (United States)

    Aparna, A; Srinikethan, G; Smitha, H

    2012-06-15

    Biosurfactant-producing bacteria were isolated from terrestrial samples collected in areas contaminated with petroleum compounds. Isolates were screened for biosurfactant production using Cetyl Tri Ammonium Bromide (CTAB)-Methylene blue agar selection medium and the qualitative drop-collapse test. An efficient bacterial strain was selected based on rapid drop collapse activity and highest biosurfactant production. The biochemical characteristics and partial sequenced 16S rRNA gene of isolate, 2B, identified the bacterium as Pseudomonas sp. Five different low cost carbon substrates were evaluated for their effect on biosurfactant production. The maximum biosurfactant synthesis (4.97 g/L) occurred at 96 h when the cells were grown on modified PPGAS medium containing 1% (v/v) molasses at 30 °C and 150 rpm. The cell free broth containing the biosurfactant could reduce the surface tension to 30.14 mN/m. The surface active compound showed emulsifying activity against a variety of hydrocarbons and achieved a maximum emulsion index of 84% for sunflower oil. Compositional analysis of the biosurfactant reveals that the extracted biosurfactant was a glycolipid type, which was composed of high percentages of lipid (∼65%, w/w) and carbohydrate (∼32%, w/w). Fourier transform infrared (FT-IR) spectrum of extracted biosurfactant indicates the presence of carboxyl, hydroxyl and methoxyl functional groups. The mass spectra (MS) shows that dirhamnolipid (l-rhamnopyranosyl-l-rhamnopyranosyl-3-hydroxydecanoyl-3-hydroxydecanoate, Rha-Rha-C(10)-C(10)) was detected in abundance with the predominant congener monorhamnolipid (l-rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydecanoate, Rha-C(10)-C(10)). The crude oil recovery studies using the biosurfactant produced by Pseudomonas sp. 2B suggested its potential application in microbial enhanced oil recovery and bioremediation. Copyright © 2012 Elsevier B.V. All rights reserved.

  14. Improved Biosurfactant Production by Bacillus subtilis SPB1 Mutant Obtained by Random Mutagenesis and Its Application in Enhanced Oil Recovery in a Sand System.

    Science.gov (United States)

    Bouassida, Mouna; Ghazala, Imen; Ellouze-Chaabouni, Semia; Ghribi, Dhouha

    2018-01-28

    Biosurfactants or microbial surfactants are surface-active biomolecules that are produced by a variety of microorganisms. Biodegradability and low toxicity have led to the intensification of scientific studies on a wide range of industrial applications for biosurfactants in the field of environmental bioremediation as well as the petroleum industry and enhanced oil recovery. However, the major issues in biosurfactant production are high production cost and low yield. Improving the bioindustrial production processes relies on many strategies, such as the use of cheap raw materials, the optimization of medium-culture conditions, and selecting hyperproducing strains. The present work aims to obtain a mutant with higher biosurfactant production through applying mutagenesis on Bacillus subtilis SPB1 using a combination of UV irradiation and nitrous acid treatment. Following mutagenesis and screening on blood agar and subsequent formation of halos, the mutated strains were examined for emulsifying activity of their culture broth. A mutant designated B. subtilis M2 was selected as it produced biosurfactant at twice higher concentration than the parent strain. The potential of this biosurfactant for industrial uses was shown by studying its stability to environmental stresses such as pH and temperature and its applicability in the oil recovery process. It was practically stable at high temperature and at a wide range of pH, and it recovered above 90% of motor oil adsorbed to a sand sample.

  15. Biosurfactant production by Mucor circinelloides on waste frying oil and possible uses in crude oil remediation.

    Science.gov (United States)

    Hasanizadeh, Parvin; Moghimi, Hamid; Hamedi, Javad

    2017-10-01

    Biosurfactants are biocompatible surface active agents which many microorganisms produce. This study investigated the production of biosurfactants by Mucor circinelloides. The effects of different factors on biosurfactant production, including carbon sources and concentrations, nitrogen sources, and iron (II) concentration, were studied and the optimum condition determined. Finally, the strain's ability to remove the crude oil and its relationship with biosurfactant production was evaluated. The results showed that M. circinelloides could reduce the surface tension of the culture medium to 26.6 mN/m and create a clear zone of 12.9 cm diameter in an oil-spreading test. The maximum surface tension reduction was recorded 3 days after incubation. The optimum condition for biosurfactant production was achieved in the presence of 8% waste frying oil as a carbon source, 2 g/L yeast extract as a nitrogen source, and 0.01 mM FeSO 4 . M. circinelloides could consume 8% waste frying oil in 5 days of incubation, and 87.6% crude oil in 12 days of incubation. A direct correlation was observed between oil degradation and surface tension reduction in the first 3 days of fungal growth. The results showed that the waste frying oil could be recommended as an inexpensive oily waste substance for biosurfactant production, and M. circinelloides could have the potential to treat waste frying oil. According to the results, the produced crude biosurfactant or fungal strain could be directly used for the mycoremediation of crude oil contamination in oil fields.

  16. Advances in utilization of renewable substrates for biosurfactant production

    Science.gov (United States)

    2011-01-01

    Biosurfactants are amphiphilic molecules that have both hydrophilic and hydrophobic moieties which partition preferentially at the interfaces such as liquid/liquid, gas/liquid or solid/liquid interfaces. Such characteristics enable emulsifying, foaming, detergency and dispersing properties. Their low toxicity and environmental friendly nature and the wide range of potential industrial applications in bioremediation, health care, oil and food processing industries makes them a highly sought after group of chemical compounds. Interest in them has also been encouraged because of the potential advantages they offer over their synthetic counterparts in many fields spanning environmental, food, biomedical, petrochemical and other industrial applications. Their large scale production and application however are currently restricted by the high cost of production and by the limited understanding of their interactions with cells and with the abiotic environment. In this paper, we review the current knowledge and latest advances in the search for cost effective renewable agro industrial alternative substrates for their production. PMID:21906330

  17. Biosurfactant production from marine hydrocarbon-degrading consortia and pure bacterial strains using crude oil as carbon source

    Directory of Open Access Journals (Sweden)

    Eleftheria eAntoniou

    2015-04-01

    Full Text Available Biosurfactants (BS are green amphiphilic molecules produced by microorganisms during biodegradation, increasing the bioavailability of organic pollutants. In this work, the BS production yield of marine hydrocarbon degraders isolated from Elefsina bay in Eastern Mediterranean Sea has been investigated. The drop collapse test was used as a preliminary screening test to confirm biosurfactant producing strains or mixed consortia. The community structure of the best consortia based on the drop collapse test was determined by 16S-rDNA pyrotag screening. Subsequently, the effect of incubation time, temperature, substrate and supplementation with inorganic nutrients, on biosurfactant production, was examined. Two types of BS - lipid mixtures were extracted from the culture broth; the low molecular weight BS Rhamnolipids and Sophorolipids. Crude extracts were purified by silica gel column chromatography and then identified by thin layer chromatography (TLC and Fourier transform infrared spectroscopy (FT-IR. Results indicate that biosurfactant production yield remains constant and low while it is independent of the total culture biomass, carbon source, and temperature. A constant BS concentration in a culture broth with continuous degradation of crude oil implies that the BS producing microbes generate no more than the required amount of biosurfactants that enables biodegradation of the crude oil. Isolated pure strains were found to have higher specific production yields than the complex microbial marine community-consortia. The heavy oil fraction of crude oil has emerged as a promising substrate for BS production (by marine BS producers with fewer impurities in the final product. Furthermore, a particular strain isolated from sediments, Paracoccus marcusii, may be an optimal choice for bioremediation purposes as its biomass remains trapped in the hydrocarbon phase, not suffering from potential dilution effects by sea currents.

  18. In-Situ Anaerobic Biosurfactant Production Process For Remediation Of DNAPL Contamination In Subsurface Aquifers

    Science.gov (United States)

    Albino, J. D.; Nambi, I. M.

    2009-12-01

    Microbial Enhanced Oil Recovery (MEOR) and remediation of aquifers contaminated with hydrophobic contaminants require insitu production of biosurfactants for mobilization of entrapped hydrophobic liquids. Most of the biosurfactant producing microorganisms produce them under aerobic condition and hence surfactant production is limited in subsurface condition due to lack of oxygen. Currently bioremediation involves expensive air sparging or excavation followed by exsitu biodegradation. Use of microorganisms which can produce biosurfactants under anaerobic conditions can cost effectively expedite the process of insitu bioremediation or mobilization. In this work, the feasibility of anaerobic biosurfactant production in three mixed anaerobic cultures prepared from groundwater and soil contaminated with chlorinated compounds and municipal sewage sludge was investigated. The cultures were previously enriched under complete anaerobic conditions in the presence of Tetrachloroethylene (PCE) for more than a year before they were studied for biosurfactant production. Biosurfactant production under anaerobic conditions was simulated using two methods: i) induction of starvation in the microbial cultures and ii) addition of complex fermentable substrates. Positive result for biosurfactant production was not observed when the cultures were induced with starvation by adding PCE as blobs which served as the only terminal electron acceptor. However, slight reduction in interfacial tension was noticed which was caused by the adherence of microbes to water-PCE interface. Biosurfactant production was observed in all the three cultures when they were fed with complex fermentable substrates and surface tension of the liquid medium was lowered below 35 mN/m. Among the fermentable substrates tested, vegetable oil yielded highest amount of biosurfactant in all the cultures. Complete biodegradation of PCE to ethylene at a faster rate was also observed when vegetable oil was amended to the

  19. Biosurfactants during in situ bioremediation: factors that influence the production and challenges in evalution.

    Science.gov (United States)

    Decesaro, Andressa; Machado, Thaís Strieder; Cappellaro, Ângela Carolina; Reinehr, Christian Oliveira; Thomé, Antônio; Colla, Luciane Maria

    2017-09-01

    Research on the influence of biosurfactants on the efficiency of in situ bioremediation of contaminated soil is continuously growing. Despite the constant progress in understanding the mechanisms involved in the effects of biosurfactants, there are still many factors that are not sufficiently elucidated. There is a lack of research on autochthonous or exogenous microbial metabolism when biostimulation or bioaugmentation is carried out to produce biosurfactants at contaminated sites. In addition, studies on the application of techniques that measure the biosurfactants produced in situ are needed. This is important because, although the positive influence of biosurfactants is often reported, there are also studies where no effect or negative effects have been observed. This review aimed to examine some studies on factors that can improve the production of biosurfactants in soils during in situ bioremediation. Moreover, this work reviews the methodologies that can be used for measuring the production of these biocomposts. We reviewed studies on the potential of biosurfactants to improve the bioremediation of hydrocarbons, as well as the limitations of methods for the production of these biomolecules by microorganisms in soil.

  20. Biosurfactant Production by Rhodococcus Erythropolis and its Application to Oil Removal

    OpenAIRE

    Pacheco, Graziela Jardim; Ciapina, Elisa Mara Prioli; Gomes, Edelvio de Barros; Junior, Nei Pereira

    2010-01-01

    The influence of different nutrients on biosurfactant production by Rhodococcus erythropolis was investigated. Increasing the concentration of phosphate buffer from 30 up through 150 mmol/L stimulated an increase in biosurfactant production, which reached a maximum concentration of 285 mg/L in shaken flasks. Statistical analysis showed that glycerol, NaNO3, MgSO4 and yeast extract had significant effects on production. The results were confirmed in a batchwise bioreactor, and semi-growth-asso...

  1. Biosurfactants production potential of native strains of Bacillus cereus and their antimicrobial, cytotoxic and antioxidant activities.

    Science.gov (United States)

    Basit, Madiha; Rasool, Muhammad Hidayat; Naqvi, Syed Ali Raza; Waseem, Muhammad; Aslam, Bilal

    2018-01-01

    Present study was designed to evaluate the biosurfactant production potential by native strains of Bacillus cereus as well as determine their antimicrobial and antioxidant activities. The strains isolated from garden soil were characterized as B. cereus MMIC 1, MMIC 2 and MMIC 3. Biosurfactants were extracted as grey white precipitates. Optimum conditions for biosurfactant production were 37°C, the 7th day of incubation, 0.5% NaCl, pH 7.0. Moreover, corn steep liquor was the best carbon source. Biuret test, Thin Layer Chromatography (TLC), agar double diffusion and Fourier Transform Infrared Spectroscopy (FTIR) characterized the biosurfactants as cationic lipopeptides. Biosurfactants exhibited significant antibacterial and antifungal activity against S. aureus, E. coli, P. aeruginosa, K. pneumoniae, A. niger and C. albicans at 30 mg/ml. Moreover, they also possessed antiviral activity against NDV at 10 mg/ml. Cytotoxicity assay in BHK-21 cell lines revealed 63% cell survival at 10 mg/ml of biosurfactants and thus considered as safe. They also showed very good antioxidant activity by ferric-reducing activity and DPPH scavenging activity at 2 mg/ml. Consequently, the study offers an insight for the exploration of new bioactive molecules from the soil. It was concluded that lipopeptide biosurfactants produced from native strains of B. cereus may be recommended as safe antimicrobial, emulsifier and antioxidant agent.

  2. Utilization of banana peel as a novel substrate for biosurfactant production by Halobacteriaceae archaeon AS65.

    Science.gov (United States)

    Chooklin, Chanika Saenge; Maneerat, Suppasil; Saimmai, Atipan

    2014-05-01

    In this study, biosurfactant-producing bacteria was evaluated for biosurfactant production by using banana peel as a sole carbon source. From the 71 strains screened, Halobacteriaceae archaeon AS65 produced the highest biosurfactant activity. The highest biosurfactant production (5.30 g/l) was obtained when the cells were grown on a minimal salt medium containing 35 % (w/v) banana peel and 1 g/l commercial monosodium glutamate at 30 °C and 200 rpm after 54 h of cultivation. The biosurfactant obtained by extraction with ethyl acetate showed high surface tension reduction (25.5 mN/m), a small critical micelle concentration value (10 mg/l), thermal and pH stability with respect to surface tension reduction and emulsification activity, and a high level of salt tolerance. The biosurfactant obtained was confirmed as a lipopeptide by using a biochemical test FT-IR, NMR, and mass spectrometry. The crude biosurfactant showed a broad spectrum of antimicrobial activity and had the ability to emulsify oil, enhance PAHs solubility, and oil bioremediation.

  3. Biosurfactant production by Rhodococcus erythropolis and its application to oil removal

    Directory of Open Access Journals (Sweden)

    Graziela Jardim Pacheco

    2010-10-01

    Full Text Available The influence of different nutrients on biosurfactant production by Rhodococcus erythropolis was investigated. Increasing the concentration of phosphate buffer from 30 up through 150 mmol/L stimulated an increase in biosurfactant production, which reached a maximum concentration of 285 mg/L in shaken flasks. Statistical analysis showed that glycerol, NaNO3,MgSO4 and yeast extract had significant effects on production. The results were confirmed in a batchwise bioreactor, and semi-growth-associated production was detected. Reduction in the surface tension, which indicates the presence of biosurfactant, reached a value of 38 mN/m at the end of 35 hours. Use of the produced biosurfactant for washing crude oil-contaminated soil showed that 2 and 4 times the critical micellar concentration (CMC were able to remove 97 and 99% of the oil, respectively, after 1 month of impregnation.

  4. PRODUCTION AND CHARACTERIZATION OF BIOSURFACTANT BY Pseudomonas fluorescens USING CASSAVA FLOUR WASTEWATER AS MEDIA

    Directory of Open Access Journals (Sweden)

    Venty Suryanti

    2013-12-01

    Full Text Available Biosurfactant with efficient emulsification properties could be produced by Pseudomonas flourescens using cassava flour wastewater (manipueira as media. The ability of P. flourescens to produce biosurfactant could suggest potential use in industrial and environmental applications. Media containing a mixture of natural manipueira and nutrient broth with 48 h fermentation was the optimum condition for the biosurfactant production. Based on UV-Vis and FT-IR spectra, the biosurfactant was indicated as rhamnolipids containing hydroxyl, ester, carboxylic and aliphatic carbon chain functional groups. Biosurfactant exhibited critical micelle concentration (CMC value of 715 mg/L and reduced the surface tension of the water from 80 mN/m to 59 mN/m. The biosurfactant was able to decrease the interfacial tension about 51-70% when benzyl chloride, palm oil and kerosene were used as water-immiscible compounds. The biosurfactant was able to form stable emulsion until 30 days when paraffin, soybean oil, lubricant oil and kerosene were used as water-immiscible compounds.

  5. Boolean models of biosurfactants production in Pseudomonas fluorescens.

    Directory of Open Access Journals (Sweden)

    Adrien Richard

    Full Text Available Cyclolipopeptides (CLPs are biosurfactants produced by numerous Pseudomonas fluorescens strains. CLP production is known to be regulated at least by the GacA/GacS two-component pathway, but the full regulatory network is yet largely unknown. In the clinical strain MFN1032, CLP production is abolished by a mutation in the phospholipase C gene (plcC and not restored by plcC complementation. Their production is also subject to phenotypic variation. We used a modelling approach with Boolean networks, which takes into account all these observations concerning CLP production without any assumption on the topology of the considered network. Intensive computation yielded numerous models that satisfy these properties. All models minimizing the number of components point to a bistability in CLP production, which requires the presence of a yet unknown key self-inducible regulator. Furthermore, all suggest that a set of yet unexplained phenotypic variants might also be due to this epigenetic switch. The simplest of these Boolean networks was used to propose a biological regulatory network for CLP production. This modelling approach has allowed a possible regulation to be unravelled and an unusual behaviour of CLP production in P. fluorescens to be explained.

  6. Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey

    OpenAIRE

    KAYA, Tayfun; ASLIM, Belma; KARİPTAŞ, Ergin

    2014-01-01

    In this study, 26 Pseudomonas spp. were isolated from a stream polluted by factory waste and from petroleum-contaminated soil. The surface tension (ST) of the cultures was used as a criterion for the primary isolation of biosurfactant-producing bacteria. Biosurfactant production was quantified by ST reduction, critical micelle concentration (CMC), emulsification capacity (EC), and cell surface hydrophobicity (CSH). Two of the isolates, P. aeruginosa 78 and 99, produced rhamnolipid biosurfacta...

  7. Screening of cloud microorganisms isolated at the Puy de Dôme (France) station for the production of biosurfactants

    Science.gov (United States)

    Renard, Pascal; Canet, Isabelle; Sancelme, Martine; Wirgot, Nolwenn; Deguillaume, Laurent; Delort, Anne-Marie

    2016-09-01

    A total of 480 microorganisms collected from 39 clouds sampled at the Puy de Dôme station (alt. 1465 m; 45°46'19'' N, 2°57'52'' E; Massif Central, France) were isolated and identified. This unique collection was screened for biosurfactant (surfactants of microbial origin) production by measuring the surface tension (σ) of the crude extracts, comprising the supernatants of the pure cultures, using the pendant drop technique. The results showed that 41 % of the tested strains were active producers (σ biosurfactant producers (σ biosurfactant production (45biosurfactants. We observed some correlations between the chemical composition of cloud water and the presence of biosurfactant-producing microorganisms, suggesting the "biogeography" of this production. Moreover, the potential impact of the production of biosurfactants by cloud microorganisms on atmospheric processes is discussed.

  8. USE OF BUTTER MILK AND POULTRY-TRANSFORMING WASTES FOR ENHANCED PRODUCTION OF Bacillus subtilis SPB1 BIOSURFACTANT IN SUBMERGED FERMENTATION

    Directory of Open Access Journals (Sweden)

    Raida Zouari

    2015-04-01

    Full Text Available Biosurfactants are valuable microbial amphiphilic molecules with effective surface-active and biological properties applicable to several industries and processes. Microorganisms synthesize them, especially during growth on water-immiscible substrates, providing an alternative to chemically prepared conventional surfactants. Microbial surfactants are not yet a sustainable alternative to chemically synthesized surfactants seeing their potentially high production charges. This study highlights the use of low-cost agro-industrial raw material for fermentative production of biosurfactants. The Box–Behnken Design and response surface methodology were employed to optimize the concentrations of the ratio butter milk /distilled water, poultry-transforming wastes and inoculum size for lipopeptide biosurfactant production by B.subtilis SPB1 in submerged fermentation.The best production yield was about 12.61 ± 0.7 g/L of crude lipopeptide biosurfactant. It can be obtained when using a ratio butter milk /distilled water of 1.5, poultry-transforming wastes of 23g/L and an inoculum size of 0.12. In comparison to the highest biosurfactant production yield reported for Bacillus subtilis SPB1, three fold increases were obtained.

  9. Production of Biosurfactants by Pseudomonas Species for Application in the Petroleum Industry.

    Science.gov (United States)

    Silva, Maria Aparecida M; Silva, Aline F; Rufino, Raquel D; Luna, Juliana M; Santos, Valdemir A; Sarubbo, Leonie A

    2017-02-01

      The production of surfactants by microorganisms has become an attractive option in the treatment of oil-contaminated environments because biosurfactants are biodegradable and less toxic than synthetic surfactants, although production costs remain high. With the aim of reducing the cost of biosurfactant production, three strains of Pseudomonas (designated P1, P2, and P3) were cultivated in a low-cost medium containing molasses and corn steep liquor as substrates. Following the selection of the best producer (P3), a rotational central composite design (RCCD) was used to determine the influence of substrates concentration on surface tension and biosurfactant yield. The biosurfactant reduced the surface tension of water to 27.5 mN/m, and its CMC was determined to be 600 mg/L. The yield was 4.0 g/L. The biosurfactant demonstrated applicability under specific environmental conditions and was able to remove 80 to 90% of motor oil adsorbed to sand. The properties of the biosurfactant suggest its potential application in bioremediation of hydrophobic pollutants.

  10. Production and properties of biosurfactants from a newly isolated Pseudomonas fluorescens HW-6 growing on hexandecane

    Energy Technology Data Exchange (ETDEWEB)

    Vasileva-Tonkova, E.; Galabova, D. [Bulgarian Academy of Sciences, Dept. of Microbial Biochemistry, Sofia (Bulgaria); Stoimenova, E.; Lalchev, Z. [Dept. of Biochemistry, Sofia Univ. ' ' St. Kliment Ohridski' ' , Sofia (Bulgaria)

    2006-07-15

    The newly isolated from industrial wastewater Pseudomonas fluorescens strain HW-6 produced glycolipid biosurfactants at high concentrations (1.4-2.0 g 1{sup -1}) when grown on hexadecane as a sole carbon source. Biosurfactants decreased the surface tension of the air/water interface by 35 mN m{sup -1} and possessed a low critical micelle concentration value of 20 mg 1{sup -1}, which indicated high surface activity. They efficiently emulsified aromatic hydrocarbons, kerosene, n-paraffins and mineral oils. Biosurfactant production contributed to a significant increase in cell hydrophobicity correlated with an increased growth of the strain on hexadecane. The results suggested that the newly isolated strain of Ps. fluorescens and produced glycolipid biosurfactants with effective surface and emulsifying properties are very promising and could find application for bioremediation of hydrocarbon-polluted sites. (orig.)

  11. Effect of Different Carbon Sources on Biosurfactants' Production by Three Strains of Lactobacillus spp.

    Science.gov (United States)

    Mouafo, Tene Hippolyte; Mbawala, Augustin; Ndjouenkeu, Robert

    2018-01-01

    The potential of three indigenous bacterial strains ( Lactobacillus delbrueckii N2, Lactobacillus cellobiosus TM1, and Lactobacillus plantarum G88) for the production of biosurfactants using sugar cane molasses or glycerol as substrates was investigated through emulsifying, surface tension, and antimicrobial activities. The different biosurfactants produced with molasses as substrate exhibited high surface tension reduction from 72 mN/m to values ranged from 47.50 ± 1.78 to 41.90 ± 0.79 mN/m and high emulsification index ranging from 49.89 ± 5.28 to 81.00 ± 1.14%. Whatever the Lactobacillus strain or the substrate used, the biosurfactants produced showed antimicrobial activities against Candida albicans LV1, some pathogenic and/or spoilage Gram-positive and Gram-negative bacteria. The yields of biosurfactants with molasses (2.43 ± 0.09 to 3.03 ± 0.09 g/L) or glycerol (2.32 ± 0.19 to 2.82 ± 0.05 g/L) were significantly ( p biosurfactants reveals that they are mainly glycoproteins and glycolipids with molasses and glycerol as substrate, respectively. Therefore, sugar cane molasses or glycerol can effectively be used by Lactobacillus strains as low-cost substrates to increase their biosurfactants production.

  12. Utilization of agroindustrial waste for biosurfactant production by native bacteria from chiapas

    Directory of Open Access Journals (Sweden)

    Yañez-Ocampo Gustavo

    2017-02-01

    Full Text Available In this work, two agro-industrial wastes, namely Waste Cooking Oil (WCO and Coffee Wastewater (CW have been used as the carbon source for the production of biosurfactants, due to their low cost and high availability. Biosurfactant-producing bacterial isolates from the Mexican state of Chiapas were used. The selected biosurfactant-producer strains were evaluated in a liquid medium with 2% (v/v of WCO as the carbon source. The assay was conducted in an Erlenmeyer flask containing 300 mL aliquots of mineral salt media (MSM + residue and incubated at 100 rpm at room temperature for 96 hours. The biosurfactant produced in the samples reduced the surface tension from 50 to 30-29 mN/m. Strains A and 83 showed the maximum emulsification index at 58-59%. Strain A showed the highest biosurfactant yield with a production of 3.7 g/L in comparison with strains B, 83 and Pseudomonas aeruginosa ATCC27853. Our results suggest that the biosurfactant produced by strain A has great potential in the treatment of wastewater with a high content of fatty acids, and of soils contaminated by pesticides or oil hydrocarbons.

  13. Biosurfactant Production by Cultivation of Bacillus atrophaeus ATCC 9372 in Semidefined Glucose/Casein-Based Media

    Science.gov (United States)

    Das Neves, Luiz Carlos Martins; de Oliveira, Kátia Silva; Kobayashi, Márcio Junji; Vessoni Penna, Thereza Christina; Converti, Attilio

    Biosurfactants are proteins with detergent, emulsifier, and antimicrobial actions that have potential application in environmental applications such as the treatment of organic pollutants and oil recovery. Bacillus atrophaeus strains are nonpathogenic and are suitable source of biosurfactants, among which is surfactin. The aim of this work is to establish a culture medium composition able to stimulate biosurfactants production by B. atrophaeus ATCC 9372. Batch cultivations were carried out in a rotary shaker at 150 rpm and 35°C for 24 h on glucose- and/or casein-based semidefined culture media also containing sodium chloride, dibasic sodium phosphate, and soy flour. The addition of 14.0 g/L glucose in a culture medium containing 10.0 g/L of casein resulted in 17 times higher biosurfactant production (B max=635.0 mg/L). Besides, the simultaneous presence of digested casein (10.0 g/L), digested soy flour (3.0 g/L), and glucose (18.0 g/L) in the medium was responsible for a diauxic effect during cell growth. Once the diauxie started, the average biosurfactants concentration was 16.8% less than that observed before this phenomenon. The capability of B. atrophaeus strain to adapt its own metabolism to use several nutrients as energy sources and to preserve high levels of biosurfactants in the medium during the stationary phase is a promising feature for its possible application in biological treatments.

  14. Effects of Au/Fe and Fe nanoparticles on Serratia bacterial growth and production of biosurfactant

    International Nuclear Information System (INIS)

    Liu, Jia; Vipulanandan, Cumaraswamy

    2013-01-01

    The overall objective of this study was to compare the effects of Au/Fe and Fe nanoparticles on the growth and performance of Serratia Jl0300. The nanoparticle effect was quantified not only by the bacterial growth on agar plate after 1 hour interaction with the nanoparticles, but also by its production of a biosurfactant from used vegetable oil. The nanoparticles were prepared using the foam method. The concentrations of the nanoparticles used for the bacterial interaction study were varied from 1 mg/L to 1 g/L. The test results showed that the effect of nanoparticles on the bacterial growth and biosurfactant production varied with nanoparticle type, concentrations, and interaction time with the bacteria. Au/Fe nanoparticles didn't show toxicity to Serratia after short time (1 h) exposure, while during 8 days fermentation Au/Fe nanoparticles inhibited the growth of Serratia as well as the biosurfactant production when the concentration of the nanoparticles was higher than 10 mg/L. Fe nanoparticles showed inhibition effects to bacterial growth both after short time and long time interaction with Serratia, as well as to biosurfactant production when its concentration was higher than 100 mg/L. Based on the trends observed in this study, analytical models have been developed to predict the bacterial growth and biosurfactant production with varying concentrations of nanoparticles. - Highlights: • Modeled the effect of nanoparticles on the bacterial growth and biosurfactant production. • Effects of Au/Fe nonoparticles on Serratia Bacterial Growth and Production of Biosurfactant. • Scanning Electron Micrograph of bacteria-nanoparticles interaction

  15. Effects of Au/Fe and Fe nanoparticles on Serratia bacterial growth and production of biosurfactant

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Jia; Vipulanandan, Cumaraswamy, E-mail: cvipulanandan@uh.edu

    2013-10-15

    The overall objective of this study was to compare the effects of Au/Fe and Fe nanoparticles on the growth and performance of Serratia Jl0300. The nanoparticle effect was quantified not only by the bacterial growth on agar plate after 1 hour interaction with the nanoparticles, but also by its production of a biosurfactant from used vegetable oil. The nanoparticles were prepared using the foam method. The concentrations of the nanoparticles used for the bacterial interaction study were varied from 1 mg/L to 1 g/L. The test results showed that the effect of nanoparticles on the bacterial growth and biosurfactant production varied with nanoparticle type, concentrations, and interaction time with the bacteria. Au/Fe nanoparticles didn't show toxicity to Serratia after short time (1 h) exposure, while during 8 days fermentation Au/Fe nanoparticles inhibited the growth of Serratia as well as the biosurfactant production when the concentration of the nanoparticles was higher than 10 mg/L. Fe nanoparticles showed inhibition effects to bacterial growth both after short time and long time interaction with Serratia, as well as to biosurfactant production when its concentration was higher than 100 mg/L. Based on the trends observed in this study, analytical models have been developed to predict the bacterial growth and biosurfactant production with varying concentrations of nanoparticles. - Highlights: • Modeled the effect of nanoparticles on the bacterial growth and biosurfactant production. • Effects of Au/Fe nonoparticles on Serratia Bacterial Growth and Production of Biosurfactant. • Scanning Electron Micrograph of bacteria-nanoparticles interaction.

  16. Biosurfactants production by yeasts using soybean oil and glycerol as low cost substrate.

    Science.gov (United States)

    Accorsini, Fábio Raphael; Mutton, Márcia Justino Rossini; Lemos, Eliana Gertrudes Macedo; Benincasa, Maria

    2012-01-01

    Biosurfactants are bioactive agents that can be produced by many different microorganisms. Among those, special attention is given to yeasts, since they can produce many types of biosurfactants in large scale, using several kinds of substrates, justifying its use for industrial production of those products. For this production to be economically viable, the use of residual carbon sources is recommended. The present study isolated yeasts from soil contaminated with petroleum oil hydrocarbons and assessed their capacity for producing biosurfactants in low cost substrates. From a microbial consortium enriched, seven yeasts were isolated, all showing potential for producing biosurfactants in soybean oil. The isolate LBPF 3, characterized as Candida antarctica, obtained the highest levels of production - with a final production of 13.86 g/L. The isolate LBPF 9, using glycerol carbon source, obtained the highest reduction in surface tension in the growth medium: approximately 43% of reduction after 24 hours of incubation. The products obtained by the isolates presented surfactant activity, which reduced water surface tension to values that varied from 34 mN/m, obtained from the product of isolates LBPF 3 and 16 LBPF 7 (respectively characterized as Candida antarctica and Candida albicans) to 43 mN/m from the isolate LPPF 9, using glycerol as substrate. The assessed isolates all showed potential for the production of biosurfactants in conventional sources of carbon as well as in agroindustrial residue, especially in glycerol.

  17. Biosurfactants production by yeasts using soybean oil and glycerol as low cost substrate

    Directory of Open Access Journals (Sweden)

    Fábio Raphael Accorsini

    2012-03-01

    Full Text Available Biosurfactants are bioactive agents that can be produced by many different microorganisms. Among those, special attention is given to yeasts, since they can produce many types of biosurfactants in large scale, using several kinds of substrates, justifying its use for industrial production of those products. For this production to be economically viable, the use of residual carbon sources is recommended. The present study isolated yeasts from soil contaminated with petroleum oil hydrocarbons and assessed their capacity for producing biosurfactants in low cost substrates. From a microbial consortium enriched, seven yeasts were isolated, all showing potential for producing biosurfactants in soybean oil. The isolate LBPF 3, characterized as Candida antarctica, obtained the highest levels of production - with a final production of 13.86 g/L. The isolate LBPF 9, using glycerol carbon source, obtained the highest reduction in surface tension in the growth medium: approximately 43% of reduction after 24 hours of incubation. The products obtained by the isolates presented surfactant activity, which reduced water surface tension to values that varied from 34 mN/m, obtained from the product of isolates LBPF 3 and 16 LBPF 7 (respectively characterized as Candida antarctica and Candida albicans to 43 mN/m from the isolate LPPF 9, using glycerol as substrate. The assessed isolates all showed potential for the production of biosurfactants in conventional sources of carbon as well as in agroindustrial residue, especially in glycerol.

  18. Production and characterization of rhamnolipid biosurfactant from waste frying coconut oil using a novel Pseudomonas aeruginosa D.

    Science.gov (United States)

    George, S; Jayachandran, K

    2013-02-01

    To improve biosurfactant production economics by the utilization of potential low-cost materials. In an attempt to utilize cost-effective carbon sources in the fermentative production of biosurfactants, various pure and waste frying oils were screened by a standard biosurfactant producing strain. Considering the regional significance, easy availability and the economical advantages, waste frying coconut oil was selected as the substrate for further studies. On isolation of more competent strains that could use waste frying coconut oil efficiently as a carbon source, six bacterial strains were isolated on cetyltrimethyl ammonium bromide-methylene blue agar plate, from a soil sample collected from the premises of a coconut oil mill. Among these, Pseudomonas aeruginosa D was selected as the potential producer of rhamnolipid. Spectrophotometric method, TLC, methylene blue active substance assay, drop collapse technique, surface tension measurement by Du Nouy ring method and emulsifying test confirmed the rhamnolipid producing ability of the selected strain and various process parameters were optimized for the production of maximum amount of biosurfactant. Rhamnolipid components purified and separated by ethyl acetate extraction, preparative silica gel column chromatography, HPLC and TLC were characterized by fast atom bombardment mass spectrometry as a mixture of dirhamnolipids and monorhamnolipids. The rhamnolipid homologues detected were Rha-Rha-C(10) -C(10) , Rha-C(12) -C(10) and Rha-C(10) -C(8) /Rha-C(8) -C(10) . These results indicated the possibility of waste frying coconut oil to be used as a very effective alternate substrate for the economic production of rhamnolipid by a newly isolated Ps. aeruginosa D. Results of this study throws light on the alternate use of already used cooking oil as high-energy source for producing a high value product like rhamnolipid. This would provide options for the food industry other than the recycling and reuse of waste frying

  19. Crude glycerol from biodiesel industry as substrate for biosurfactant production by Bacillus subtilis ATCC 6633

    Directory of Open Access Journals (Sweden)

    Marylane de Sousa

    2014-04-01

    Full Text Available Glycerol, a co-product of the biodiesel industry, may be a suitable raw material for the production of high added-value compounds by the microorganisms. This study aimed to use the glycerol obtained from the biodiesel production process as the main carbon source for biosurfactant production by Bacillus subtilis ATCC 6633. Results indicated that the strain lowered the surface tension of the cell-free fermented broth to 31.5 ± 1.6 mN/m, indicating the production of biosurfactant. The critical micelle concentration (CMC = 33.6 mN/m obtained was similar to the previously reported for biossurfactants isolated from other Bacillus. The produced biosurfactant was able to emulsify n-hexadecane and soybean oil.

  20. Estudo da produção de biossurfactante em caldo de fermentação Study on the production of biosurfactant fermentation broth

    Directory of Open Access Journals (Sweden)

    Silvia Messias Bueno

    2010-01-01

    Full Text Available A bacterium isolated from soil contaminated by hydrocarbon was studied and, by biochemical tests and analysis of PCR, the presence of Bacillus pumilus was identified. The production of biosurfactant was optimized, test of oil degradation and antimicrobial activity determination. The results showed that pH 5.0 and 7.0, 72 h of fermentation, sucrose and sugar cane juice (2% had best yields. The bacterium is able to degrade crude oil and displays bacteriostatic and fungistatic activity. From the analysis of proximate composition of biosurfactant found the presence of biopolymer formed by a lipopolysaccharide-protein complex.

  1. Simultaneous production of lipases and biosurfactants by submerged and solid-state bioprocesses.

    Science.gov (United States)

    Colla, Luciane Maria; Rizzardi, Juliana; Pinto, Marta Heidtmann; Reinehr, Christian Oliveira; Bertolin, Telma Elita; Costa, Jorge Alberto Vieira

    2010-11-01

    Lipases and biosurfactants are compounds produced by microorganisms generally involved in the metabolization of oil substrates. However, the relationship between the production of lipases and biosurfactants has not been established yet. Therefore, this study aimed to evaluate the correlation between production of lipases and biosurfactants by submerged (SmgB) and solid-state bioprocess (SSB) using Aspergillus spp., which were isolated from a soil contaminated by diesel oil. SSB had the highest production of lipases, with lipolytic activities of 25.22U, while SmgB had 4.52U. The production of biosurfactants was not observed in the SSB. In the SmgB, correlation coefficients of 91% and 87% were obtained between lipolytic activity and oil in water and water in oil emulsifying activities, respectively. A correlation of 84% was obtained between lipolytic activity and reduction of surface tension in the culture medium. The surface tension decreased from 50 to 28mNm(-1) indicating that biosurfactants were produced in the culture medium. Copyright 2010 Elsevier Ltd. All rights reserved.

  2. Use of weathered diesel oil as a low-cost raw material for biosurfactant production

    Directory of Open Access Journals (Sweden)

    A. P. Mariano

    2008-06-01

    Full Text Available This work aimed to investigate the capability of biosurfactant production by Staphylococcus hominis, Kocuria palustris and Pseudomonas aeruginosa LBI, using weathered diesel oil from a long-standing spillage as raw material. The effect of the culture media (Robert or Bushnell-Haas and of the carbon source (spilled diesel oil or commercial diesel oil on biosurfactant production was evaluated. Erlenmeyer flasks (250 mL containing the cell broth were agitated (240 rpm for 144 h at 27±2ºC. Biosurfactant production was monitored according to the De Nöuy ring method using a Krüss K6 tensiometer. Considering the possibility of intracellular storage of biosurfactant in the cell wall of the cultures S. hominis and K. palustris, experiments were also done applying ultrasound as a way to rupture the cells. For the conditions studied, the cultures did not indicate production of biosurfactants. Results obtained with a hydrocarbon biodegradability test based on the redox indicator 2,6-dichlorophenol indophenol showed that only the commercial diesel was biodegraded by the cultures.

  3. Biosurfactants production and possible uses in microbial enhanced oil recovery and oil pollution remediation: a review

    International Nuclear Information System (INIS)

    Banat, I.M.

    1995-01-01

    Surfactants are widely used for various purposes in industry, but for many years were mainly chemically synthesized. It has only been in the past few decades that biological surface-active compounds (biosurfactants) have been described. Biosurfactants are gaining prominence and have already taken over for a number of important industrial uses, due to their advantages of biodegradability, production on renewable resources and functionality under extreme conditions; particularly those pertaining during tertiary crude-oil recovery. Conflicting reports exist concerning their efficacy and the economics of both their production and application. The limited successes and applications for biosurfactants production, recovery, use in oil pollution control, oil storage tank clean-up and enhanced oil-recovery are reviewed from the technical point of view. (author)

  4. Biosurfactant-biopolymer driven microbial enhanced oil recovery (MEOR) and its optimization by an ANN-GA hybrid technique.

    Science.gov (United States)

    Dhanarajan, Gunaseelan; Rangarajan, Vivek; Bandi, Chandrakanth; Dixit, Abhivyakti; Das, Susmita; Ale, Kranthikiran; Sen, Ramkrishna

    2017-08-20

    A lipopeptide biosurfactant produced by marine Bacillus megaterium and a biopolymer produced by thermophilic Bacillus licheniformis were tested for their application potential in the enhanced oil recovery. The crude biosurfactant obtained after acid precipitation effectively reduced the surface tension of deionized water from 70.5 to 28.25mN/m and the interfacial tension between lube oil and water from 18.6 to 1.5mN/m at a concentration of 250mgL -1 . The biosurfactant exhibited a maximum emulsification activity (E 24 ) of 81.66% against lube oil. The lipopeptide micelles were stabilized by addition of Ca 2+ ions to the biosurfactant solution. The oil recovery efficiency of Ca 2+ conditioned lipopeptide solution from a sand-packed column was optimized by using artificial neural network (ANN) modelling coupled with genetic algorithm (GA) optimization. Three important parameters namely lipopeptide concentration, Ca 2+ concentration and solution pH were considered for optimization studies. In order to further improve the recovery efficiency, a water soluble biopolymer produced by Bacillus licheniformis was used as a flooding agent after biosurfactant incubation. Upon ANN-GA optimization, 45% tertiary oil recovery was achieved, when biopolymer at a concentration of 3gL -1 was used as a flooding agent. Oil recovery was only 29% at optimal conditions predicted by ANN-GA, when only water was used as flooding solution. The important characteristics of biopolymers such as its viscosity, pore plugging capabilities and bio-cementing ability have also been tested. Thus, as a result of biosurfactant incubation and biopolymer flooding under the optimal process conditions, a maximum oil recovery of 45% was achieved. Therefore, this study is novel, timely and interesting for it showed the combined influence of biosurfactant and biopolymer on solubilisation and mobilization of oil from the soil. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. Microorganism selection and biosurfactant production in a continuously and periodically operated bioslurry reactor.

    Science.gov (United States)

    Cassidy, D P; Hudak, A J

    2001-06-29

    A continuous-flow reactor (CSTR) and a soil slurry-sequencing batch reactor (SS-SBR) were maintained in 8l vessels for 180 days to treat a soil contaminated with diesel fuel (DF). Concentrations of Candida tropicalis, Brevibacterium casei, Flavobacterium aquatile, Pseudomonas aeruginosa, and Pseudomonas fluorescens were determined using fatty acid methyl ester (FAME) analysis. DF removal (biological and volatile) and biosurfactant concentrations were measured. The SS-SBR encouraged the growth of biosurfactant-producing species relative to the CSTR. Counts of biosurfactant-producing species (C. tropicalis, P. aeruginosa, P. fluorescens) relative to total microbial counts were 88% in the SS-SBR and 23% in the CSTR. Biosurfactants were produced in the SS-SBR to levels of nearly 70 times the critical micelle concentration (CMC) early in the cycle, but were completely degraded by the end of each cycle. No biosurfactant production was observed in the CSTR. DF biodegradation rates were over 40% greater and DF stripping was over five times lower in the SS-SBR than the CSTR. However, considerable foaming occurred in the SS-SBR. Reversing the mode of operation in the reactors on day 80 caused a complete reversal in microbial consortia and reactor performance by day 120. These results show that bioslurry reactor operation can be manipulated to control overall reactor performance.

  6. Production and characterization of a biosurfactant produced by Streptomyces sp. DPUA 1559 isolated from lichens of the Amazon region.

    Science.gov (United States)

    Santos, A P P; Silva, M D S; Costa, E V L; Rufino, R D; Santos, V A; Ramos, C S; Sarubbo, L A; Porto, A L F

    2017-12-11

    Surfactants are amphipathic compounds containing both hydrophilic and hydrophobic groups, capable to lower the surface or interfacial tension. Considering the advantages of the use of biosurfactants produced by microorganisms, the aim of this paper was to develop and characterize a biosurfactant produced by Streptomyces sp. DPUA1559 isolated from lichens of the Amazon region. The microorganism was cultured in a mineral medium containing 1% residual frying soybean oil as the carbon source. The kinetics of biosurfactant production was accompanied by reducing the surface tension of the culture medium from 60 to values around 27.14 mN/m, and by the emulsification index, which showed the efficiency of the biosurfactant as an emulsifier of hydrophobic compounds. The yield of the isolated biosurfactant was 1.74 g/L, in addition to the excellent capability of reducing the surface tension (25.34 mN/m), as observed from the central composite rotational design when the biosurfactant was produced at pH 8.5 at 28°C. The critical micelle concentration of the biosurfactant was determined as 0.01 g/mL. The biosurfactant showed thermal and pH stability regarding the surface tension reduction, and tolerance under high salt concentrations. The isolated biosurfactant showed no toxicity to the micro-crustacean Artemia salina, and to the seeds of lettuce (Lactuca sativa L.) and cabbage (Brassica oleracea L.). The biochemistry characterization of the biosurfactant showed a single protein band, an acid character and a molecular weight around 14.3 kDa, suggesting its glycoproteic nature. The results are promising for the industrial application of this new biosurfactant.

  7. Production of microbial biosurfactants: Status quo of rhamnolipid and surfactin towards large-scale production.

    Science.gov (United States)

    Henkel, Marius; Geissler, Mareen; Weggenmann, Fabiola; Hausmann, Rudolf

    2017-07-01

    Surfactants are an important class of industrial chemicals. Nowadays oleochemical surfactants such as alkyl polyglycosides (APGs) become increasingly important. This trend towards the utilization of renewable resources continues and consumers increasingly demand for environmentally friendly products. Consequently, research in microbial surfactants has drastically increased in the last years. While for mannosylerythritol lipids and sophorolipids established industrial processes exist, an implementation of other microbially derived surfactants has not yet been achieved. Amongst these biosurfactants, rhamnolipids synthesized by Pseudomonas aeruginosa and surfactin produced by Bacillus subtilis are so far the most analyzed biosurfactants due to their exceptional properties and the concomitant possible applications. In this review, a general overview is given regarding the current status of biosurfactants and benefits attributed to these molecules. Furthermore, the most recent research approaches for both rhamnolipids and surfactin are presented with respect to possible methods for industrial processes and the occurring drawbacks and limitations researchers have to address and overcome. Copyright © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  8. Development of an In Situ Biosurfactant Production Technology for Enhanced Oil Recovery

    Energy Technology Data Exchange (ETDEWEB)

    M.J. McInerney; R.M. Knapp; Kathleen Duncan; D.R. Simpson; N. Youssef; N. Ravi; M.J. Folmsbee; T.Fincher; S. Maudgalya; Jim Davis; Sandra Weiland

    2007-09-30

    The long-term economic potential for enhanced oil recovery (EOR) is large with more than 300 billion barrels of oil remaining in domestic reservoirs after conventional technologies reach their economic limit. Actual EOR production in the United States has never been very large, less than 10% of the total U. S. production even though a number of economic incentives have been used to stimulate the development and application of EOR processes. The U.S. DOE Reservoir Data Base contains more than 600 reservoirs with over 12 billion barrels of unrecoverable oil that are potential targets for microbially enhanced oil recovery (MEOR). If MEOR could be successfully applied to reduce the residual oil saturation by 10% in a quarter of these reservoirs, more than 300 million barrels of oil could be added to the U.S. oil reserve. This would stimulate oil production from domestic reservoirs and reduce our nation's dependence on foreign imports. Laboratory studies have shown that detergent-like molecules called biosurfactants, which are produced by microorganisms, are very effective in mobilizing entrapped oil from model test systems. The biosurfactants are effective at very low concentrations. Given the promising laboratory results, it is important to determine the efficacy of using biosurfactants in actual field applications. The goal of this project is to move biosurfactant-mediated oil recovery from laboratory investigations to actual field applications. In order to meet this goal, several important questions must be answered. First, it is critical to know whether biosurfactant-producing microbes are present in oil formations. If they are present, then it will be important to know whether a nutrient regime can be devised to stimulate their growth and activity in the reservoir. If biosurfactant producers are not present, then a suitable strain must be obtained that can be injected into oil reservoirs. We were successful in answering all three questions. The specific

  9. Utilization of Paneer Whey Waste for Cost-Effective Production of Rhamnolipid Biosurfactant.

    Science.gov (United States)

    Patowary, Rupshikha; Patowary, Kaustuvmani; Kalita, Mohan Chandra; Deka, Suresh

    2016-10-01

    The present study aimed at isolating rhamnolipid biosurfactant-producing bacteria that could utilize paneer whey, an abundant waste source as sole medium for the production purpose. Pseudomonas aeruginosa strain, SR17, was isolated from hydrocarbon-contaminated soil that could efficiently utilize paneer whey for rhamnolipid production and reduce surface tension of the medium from 52 to 26.5 mN/m. The yield of biosurfactant obtained was 2.7 g/l, upgraded to 4.8 g/l when supplemented with 2 % glucose and mineral salts. Biochemical, FTIR, and LC-MS analysis revealed that extracted biosurfactant is a combination of both mono and di-rhamnolipid congeners. The critical micelle concentration (CMC) was measured to be 110 mg/l. Emulsification activity of the biosurfactant against n-hexadecane, olive oil, kerosene, diesel oil, engine oil, and crude oil were found to be 83, 88, 81, 92, 86, and 100 %, respectively. The rhamnolipid was detected to be non-toxic against mouse fibroblastic cell line L292.

  10. Petroleum Product as Substrate for the Production of Biosurfactive ...

    African Journals Online (AJOL)

    Bashir

    Full Length R esearch A rticle ... specific characteristics of some of these ... industries; large scale production remains economically challenging ... h = height of the liquid in the column (m) g = gravity .... soluble hydrocarbon may also influence the rate and quantity .... Liu, J.F., Mbadinga, S.M., Yang, S.Z., Gu, J.D. and Mu, B.Z. ...

  11. Biosurfactants in agriculture.

    Science.gov (United States)

    Sachdev, Dhara P; Cameotra, Swaranjit S

    2013-02-01

    Agricultural productivity to meet growing demands of human population is a matter of great concern for all countries. Use of green compounds to achieve the sustainable agriculture is the present necessity. This review highlights the enormous use of harsh surfactants in agricultural soil and agrochemical industries. Biosurfactants which are reported to be produced by bacteria, yeasts, and fungi can serve as green surfactants. Biosurfactants are considered to be less toxic and eco-friendly and thus several types of biosurfactants have the potential to be commercially produced for extensive applications in pharmaceutical, cosmetics, and food industries. The biosurfactants synthesized by environmental isolates also has promising role in the agricultural industry. Many rhizosphere and plant associated microbes produce biosurfactant; these biomolecules play vital role in motility, signaling, and biofilm formation, indicating that biosurfactant governs plant-microbe interaction. In agriculture, biosurfactants can be used for plant pathogen elimination and for increasing the bioavailability of nutrient for beneficial plant associated microbes. Biosurfactants can widely be applied for improving the agricultural soil quality by soil remediation. These biomolecules can replace the harsh surfactant presently being used in million dollar pesticide industries. Thus, exploring biosurfactants from environmental isolates for investigating their potential role in plant growth promotion and other related agricultural applications warrants details research. Conventional methods are followed for screening the microbial population for production of biosurfactant. However, molecular methods are fewer in reaching biosurfactants from diverse microbial population and there is need to explore novel biosurfactant from uncultured microbes in soil biosphere by using advanced methodologies like functional metagenomics.

  12. Utilization of palm oil mill effluent as a novel and promising substrate for biosurfactant production by Nevskia ramosa NA3

    Directory of Open Access Journals (Sweden)

    Benjamas Cheirsilp

    2013-04-01

    Full Text Available This paper introduces palm oil mill effluent as a promising substrate for biosurfactant production. Potential strains ofbacteria were isolated from various hydrocarbon-contaminated soils and screened for biosurfactant production with the helpof the drop collapse method and surface tension measurements. Out of 26 isolates of bacteria, the strain NA3 showed thehighest bacterial growth with the highest surface tension reduction of 27.2 mN/m. It was then identified as Nevskia ramosaNA3 by biochemical and 16S rRNA sequence analysis. The Plackett-Burman experimental design was employed to determinethe important nutritional requirements for biosurfactant production by N. ramosa NA3 under controlled conditions. Six outof 11 factors of the production medium were found to significantly affect the production of biosurfactant. FeCl2 and NaNO3had a direct proportional correlation with the biosurfactant production. Commercial sugar, glucose, K2HPO4 and MgCl2showed inversely proportional relationship with biosurfactant production in the selected experimental range.

  13. Production of Lipopeptide Biosurfactant by a Marine Nesterenkonia sp. and Its Application in Food Industry

    Science.gov (United States)

    Kiran, George S.; Priyadharsini, Sethu; Sajayan, Arya; Priyadharsini, Gopal B.; Poulose, Navya; Selvin, Joseph

    2017-01-01

    Biosurfactants are smart biomolecules which have wide spread application in medicines, processed foods, cosmetics as well as in bioremediation. In food industry, biosurfactants are used as emulsion stabilizing agents, antiadhesives, and antimicrobial/antibiofilm agents. Nowadays biosurfactant demands in industries has increased tremendously and therefore new bacterial strains are being explored for large scale production of biosurfactants. In this study, an actinobacterial strain MSA31 was isolated from a marine sponge Fasciospongia cavernosa which showed high activity in biosurfactant screening assays such as drop collapsing, oil displacement, lipase and emulsification. Lipopeptide produced by MSA31 was found to be thermostable which was evident in differential scanning calorimetry analysis. The spectral data obtained in the Fourier transform infrared spectroscopy showed the presence of aliphatic groups combined with peptide moiety which is a characteristic feature of lipopeptides. The stability index of lipopeptide MSA31 revealed “halo-alkali and thermal tolerant biosurfactant” which can be used in the food industry. Microtiter plate assay showed 125 μg/ml of lipopeptide was effective in reducing the biofilm formation activity of pathogenic multidrug resistant Staphylococcus aureus. The confocal laser scanning microscopic images provided further evidences that lipopeptide MSA31 was an effective antibiofilm agent. The antioxidant activity of lipopeptide MSA31 may be due to the presence of unsaturated fatty acid present in the molecule. The brine shrimp cytotoxicity assay showed lipopeptide MSA31 was non-toxic and can be used as food additives. Incorporation of lipopeptide MSA31 in muffin showed improved organoleptic qualities compared to positive and negative control. This study provides a valuable input for this lipopeptide to be used in food industry as an effective emulsifier, with good antioxidant activity and as a protective agent against S. aureus. PMID

  14. Production of Lipopeptide Biosurfactant by a Marine Nesterenkonia sp. and Its Application in Food Industry

    Directory of Open Access Journals (Sweden)

    George S. Kiran

    2017-06-01

    Full Text Available Biosurfactants are smart biomolecules which have wide spread application in medicines, processed foods, cosmetics as well as in bioremediation. In food industry, biosurfactants are used as emulsion stabilizing agents, antiadhesives, and antimicrobial/antibiofilm agents. Nowadays biosurfactant demands in industries has increased tremendously and therefore new bacterial strains are being explored for large scale production of biosurfactants. In this study, an actinobacterial strain MSA31 was isolated from a marine sponge Fasciospongia cavernosa which showed high activity in biosurfactant screening assays such as drop collapsing, oil displacement, lipase and emulsification. Lipopeptide produced by MSA31 was found to be thermostable which was evident in differential scanning calorimetry analysis. The spectral data obtained in the Fourier transform infrared spectroscopy showed the presence of aliphatic groups combined with peptide moiety which is a characteristic feature of lipopeptides. The stability index of lipopeptide MSA31 revealed “halo-alkali and thermal tolerant biosurfactant” which can be used in the food industry. Microtiter plate assay showed 125 μg/ml of lipopeptide was effective in reducing the biofilm formation activity of pathogenic multidrug resistant Staphylococcus aureus. The confocal laser scanning microscopic images provided further evidences that lipopeptide MSA31 was an effective antibiofilm agent. The antioxidant activity of lipopeptide MSA31 may be due to the presence of unsaturated fatty acid present in the molecule. The brine shrimp cytotoxicity assay showed lipopeptide MSA31 was non-toxic and can be used as food additives. Incorporation of lipopeptide MSA31 in muffin showed improved organoleptic qualities compared to positive and negative control. This study provides a valuable input for this lipopeptide to be used in food industry as an effective emulsifier, with good antioxidant activity and as a protective agent

  15. Biofilm lifestyle enhances diesel bioremediation and biosurfactant production in the Antarctic polyhydroxyalkanoate producer Pseudomonas extremaustralis.

    Science.gov (United States)

    Tribelli, Paula M; Di Martino, Carla; López, Nancy I; Raiger Iustman, Laura J

    2012-09-01

    Diesel is a widely distributed pollutant. Bioremediation of this kind of compounds requires the use of microorganisms able to survive and adapt to contaminated environments. Pseudomonas extremaustralis is an Antarctic bacterium with a remarkable survival capability associated to polyhydroxyalkanoates (PHAs) production. This strain was used to investigate the effect of cell growth conditions--in biofilm versus shaken flask cultures--as well as the inocula characteristics associated with PHAs accumulation, on diesel degradation. Biofilms showed increased cell growth, biosurfactant production and diesel degradation compared with that obtained in shaken flask cultures. PHA accumulation decreased biofilm cell attachment and enhanced biosurfactant production. Degradation of long-chain and branched alkanes was observed in biofilms, while in shaken flasks only medium-chain length alkanes were degraded. This work shows that the PHA accumulating bacterium P. extremaustralis can be a good candidate to be used as hydrocarbon bioremediation agent, especially in extreme environments.

  16. Biosurfactants' Production from Renewable Natural Resources: Example of Innovativeand Smart Technology in Circular Bioeconomy

    Science.gov (United States)

    Satpute, Surekha K.; Płaza, Grażyna A.; Banpurkar, Arun G.

    2017-03-01

    A strong developed bio-based industrial sector will significantly reduce dependency on fossil resources, help the countries meet climate change targets, and lead to greener and more environmental friendly growth. The key is to develop new technologies to sustainably transform renewable natural resources into bio-based products and biofuels. Biomass is a valuable resource and many parameters need to be taken in to account when assessing its use and the products made from its. The bioeconomy encompass the production of renewable biological resources and their conversion into food, feed and bio-based products (chemicals, materials and fuels) via innovative and efficient technologies provided by industrial biotechnology. The paper presents the smart and efficient way to use the agro-industrial, dairy and food processing wastes for biosurfactant's production. Clarification processes are mandatory to use the raw substrates for microbial growth as well as biosurfactant production for commercial purposes. At the same time it is very essential to retain the nutritional values of those cheap substrates. Broad industrial perspectives can be achieved when quality as well as the quantity of the biosurfactant is considered in great depth. Since substrates resulting from food processing, dairy, animal fat industries are not explored in great details; and hence are potential areas which can be explored thoroughly.

  17. Biosurfactant production by hydrocarbon-degrading Brevibacterium and Vibrio isolates from the sea pen Pteroeides spinosum (Ellis, 1764).

    Science.gov (United States)

    Graziano, Marco; Rizzo, Carmen; Michaud, Luigi; Porporato, Erika Maria Diletta; De Domenico, Emilio; Spanò, Nunziacarla; Lo Giudice, Angelina

    2016-09-01

    Among filter-feeders, pennatulids are the most complex and polymorphic members of the cnidarian class Anthozoa. They display a wide distribution throughout all the oceans, constituting a significant component of the sessile megafauna from intertidal to abyssal depths. In this study, a total of 118 bacterial isolates from enrichment cultures, carried out with homogenates of the sea pen Pteroeides spinosum (Ellis, 1764), were screened for hydrocarbon utilization by using the 2,6-dichlorophenol indophenol assay. Among them, 83 hydrocarbon-oxidizing isolates were analyzed for biosurfactant production by standard screening tests (i.e., emulsifying activity, E24 detection, surface tension measurement, microplate assay). The 16S rRNA gene sequencing revealed the affiliation of the most promising isolates to the genera Brevibacterium and Vibrio. Biosurfactant production resulted strongly affected by salinity and temperature conditions, and occurred in the presence of diesel oil and/or crude oil, whereas no production was observed when isolates were grown on tetradecane. The strains resulted able to create stable emulsions, thus suggesting the production of biosurfactants. Further analyses revealed a glycolipidic nature of the biosurfactant extracted from Vibrio sp. PBN295, a genus that has been only recently reported as biosurfactant producer. Results suggest that pennatulids could represent a novel source for the isolation of hydrocarbon-oxidizing bacteria with potential in biosurfactant production. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. Biosurfactants for microbubble preparation and application

    Science.gov (United States)

    Biosurfactants can be classified by their chemical composition and their origin. This review briefly describes the type of biosurfactants based on their origin. Some of the widely used biosurfactants are introduced. The current statues and future trends in the production of biosurfactants are discus...

  19. Biosurfactant production from marine hydrocarbon-degrading consortia and pure bacterial strains using crude oil as carbon source

    OpenAIRE

    Antoniou, Eleftheria; Fodelianakis, Stilianos; Korkakaki, Emmanouela; Kalogerakis, Nicolas

    2015-01-01

    Biosurfactants (BS) are green amphiphilic molecules produced by microorganisms during biodegradation, increasing the bioavailability of organic pollutants. In this work, the BS production yield of marine hydrocarbon degraders isolated from Elefsina bay in Eastern Mediterranean Sea has been investigated. The drop collapse test was used as a preliminary screening test to confirm biosurfactant producing strains or mixed consortia. The community structure of the best consortia based on the drop c...

  20. Isolation and characterization of a biosurfactant-producing Fusarium sp. BS-8 from oil contaminated soil.

    Science.gov (United States)

    Qazi, Muneer A; Kanwal, Tayyaba; Jadoon, Muniba; Ahmed, Safia; Fatima, Nighat

    2014-01-01

    This study reports characterization of a biosurfactant-producing fungal isolate from oil contaminated soil of Missa Keswal oil field, Pakistan. It was identified as Fusarium sp. BS-8 on the basis of macroscopic and microscopic morphology, and 18S rDNA gene sequence homology. The biosurfactant-producing capability of the fungal isolates was screened using oil displacement activity, emulsification index assay, and surface tension (SFT) measurement. The optimization of operational parameters and culture conditions resulted in maximum biosurfactant production using 9% (v/v) inoculum at 30°C, pH 7.0, using sucrose and yeast extract, as carbon and nitrogen sources, respectively. A C:N ratio of 0.9:0.1 (w/w) was found to be optimum for growth and biosurfactant production. At optimal conditions, it attained lowest SFT (i.e., 32 mN m(-1) ) with a critical micelle concentration of ≥ 1.2 mg mL(-1) . During 5 L shake flask fermentation experiments, the biosurfactant productivity was 1.21 g L(-1) pure biosurfactant having significant emulsifying index (E24 , 70%) and oil-displacing activity (16 mm). Thin layer chromatography and Fourier transform infrared spectrometric analyses indicated a lipopeptide type of the biosurfactant. The Fusarium sp. BS-8 has substantial potential of biosurfactant production, yet it needs to be fully characterized with possibility of relatively new class of biosurfactants. © 2014 American Institute of Chemical Engineers.

  1. Bioaugmentation of oil reservoir indigenous Pseudomonas aeruginosa to enhance oil recovery through in-situ biosurfactant production without air injection.

    Science.gov (United States)

    Zhao, Feng; Li, Ping; Guo, Chao; Shi, Rong-Jiu; Zhang, Ying

    2018-03-01

    Considering the anoxic conditions within oil reservoirs, a new microbial enhanced oil recovery (MEOR) technology through in-situ biosurfactant production without air injection was proposed. High-throughput sequencing data revealed that Pseudomonas was one of dominant genera in Daqing oil reservoirs. Pseudomonas aeruginosa DQ3 which can anaerobically produce biosurfactant at 42 °C was isolated. Strain DQ3 was bioaugmented in an anaerobic bioreactor to approximately simulate MEOR process. During bioaugmentation process, although a new bacterial community was gradually formed, Pseudomonas was still one of dominant genera. Culture-based data showed that hydrocarbon-degrading bacteria and biosurfactant-producing bacteria were activated, while sulfate reducing bacteria were controlled. Biosurfactant was produced at simulated reservoir conditions, decreasing surface tension to 33.8 mN/m and emulsifying crude oil with EI 24  = 58%. Core flooding tests revealed that extra 5.22% of oil was displaced by in-situ biosurfactant production. Bioaugmenting indigenous biosurfactant producer P. aeruginosa without air injection is promising for in-situ MEOR applications. Copyright © 2017 Elsevier Ltd. All rights reserved.

  2. Comparison of some indigenous bacterial strains of pseudomonas ssp. for production of biosurfactants

    International Nuclear Information System (INIS)

    Sahafeeq, M.; Kokub, D.; Khalid, Z.M.; Malik, K.A.

    1991-01-01

    Some indigenous pseudomonas spp. were found to have the ability of emulsification, lowering the surface and interfacial tensions, and formation of high reciprocal CMCs. Six strains of Pseudomonas spp were compared for biosurfactant production grown on hexadecane. Supernatant from whole culture broth of these strains could lower surface tension from 65 mN/m to 28-32 nM/m, interfacial tension from 40 nM/m to 1-3 mN/m and had high reciprocal CMCs. When compared for emulsification ability by the culture broth of these strains, the emulsification index (E24) was found to range between 60-65. Biosurfactant containing culture broth of some strains could retain the property up to 80 C, pH of 13 and sodium chloride concentration for 17% which indicates their possible role in some depleted oil well. (author)

  3. Biosurfactants research trends and applications

    CERN Document Server

    Mulligan, Catherine N; Sharma, Sanjay K

    2014-01-01

    Green chemistry and Biosurfactant ResearchCatherine N. Mulligan, Sanjay K. Sharma, Ackmez Mudhoo,and Komal MakhijaniAmphiphilic Molecules of Microbial Origin: Classification, Characteristics, Genetic Regulations, and Pathways for BiosynthesisGunaseelan Dhanarajan and Ramkrishna SenRhamnolipids: Characteristics, Production, Applications, and AnalysisFereshteh Arab and Catherine N. MulliganSophorolipids: Characteristics, Production, and ApplicationsVivek K. Morya and Eun-Ki KimBiosurfactants and Bioemulsifiers from Marine SourcesRengathavasi Thavasi and Ibrahim M. BanatCharacterization, Production, and Applications of LipopeptidesCatherine N. MulliganBiosurfactants in the Food IndustryMarcia Nitschke and Siddhartha G.V.A.O. CostaTrehalose BiosurfactantsNelly Christova and Ivanka StoinevaBiosurfactant-Mediated Nanoparticle Synthesis: A Green and SustainableApproachVivek Rangarajan, Snigdha Majumder, and Ramkrishna SenEnhancement of Remediation Technologies with BiosurfactantsCatherine N. MulliganBiosurfactant Co...

  4. Biosurfactant production by the crude oil degrading Stenotrophomonas sp. B-2: chemical characterization, biological activities and environmental applications.

    Science.gov (United States)

    Gargouri, Boutheina; Contreras, María Del Mar; Ammar, Sonda; Segura-Carretero, Antonio; Bouaziz, Mohamed

    2017-02-01

    In this work, biosurfactant-producing microorganisms were isolated from hydrocarbon-contaminated water collected from Tunisian oilfield. After enrichment and isolation, different bacterial strains were preliminary studied for their biosurfactant/bioemulsifier properties when using crude oil as the unique carbon source. In particular, the isolate strain B-2, a Gram-negative, rod-shaped bacterium, efficiently emulsified crude oil. The extracellular biosurfactant product from this strain presented an emulsification activity above 70% and a hydrophobicity of 71%. In addition, a diameter of 6 cm was observed in the oil displacement test. The characterization of B-2 strain using 16S rDNA sequencing enables us to find a high degree of similarity with various members of the genus Stenotrophomonas (with a percentage of similarity of 99%). The emulsification activity of Stenotrophomonas biosurfactant B-2 was maintained in a wide range of pH (2 to 6), temperature (4 to 55 °C), and salinity (0 to 50 g L -1 ) conditions. It also enhanced the solubility of phenanthrene in water and could be used in the re-mobilization of hydrocarbon-contaminated environment. In addition, this biosurfactant exhibited antimicrobial and antioxidant properties. Infrared spectroscopy suggested potential lipidic and peptidic moieties, and mass spectrometry-based analyses showed that the biosurfactant contains mainly cyclic peptidic structures belonging to the class of diketopiperazines. Therefore, the B-2 strain is a promising biosurfactant-producing microorganism and its derived biosurfactant presents a wide range of industrial applications.

  5. Simultaneous Production of Biosurfactants and Bacteriocins by Probiotic Lactobacillus casei MRTL3

    Science.gov (United States)

    Sharma, Deepansh; Singh Saharan, Baljeet

    2014-01-01

    Lactic acid bacteria (LAB) are ubiquitous and well-known commensal bacteria in the human and animal microflora. LAB are extensively studied and used in a variety of industrial and food fermentations. They are widely used for humans and animals as adjuvants, probiotic formulation, and dietary supplements and in other food fermentation applications. In the present investigation, LAB were isolated from raw milk samples collected from local dairy farms of Haryana, India. Further, the isolates were screened for simultaneous production of biosurfactants and bacteriocins. Biosurfactant produced was found to be a mixture of lipid and sugar similar to glycolipids. The bacteriocin obtained was found to be heat stable (5 min at 100°C). Further, DNA of the strain was extracted and amplified by the 16S rRNA sequencing using universal primers. The isolate Lactobacillus casei MRTL3 was found to be a potent biosurfactant and bacteriocin producer. It seems to have huge potential for food industry as a biopreservative and/or food ingredient. PMID:24669225

  6. Biosurfactant production by Pseudomonas aeruginosa MSIC02 in cashew apple juice using a 24 full factorial experimental design

    Directory of Open Access Journals (Sweden)

    Rocha Maria Valderez Ponte

    2014-01-01

    Full Text Available In this work, the production of biosurfactants from cashew apple juice by P. aeruginosa MSIC02 was investigate by carrying out a 24 full factorial experimental design, using temperature, glucose concentration from cashew apple juice, phosphorous concentration and cultivation time as variables. The response variable was the percentage of reduction in surface tension in the cell-free culture medium, since it indicates the surface-active agent production. Maximum biosurfactant production, equivalent to a 58% reduction in surface tension, was obtained at 37°C, with glucose concentration of 5.0 g/L and no phosphorous supplementation. Surface tension reduction was significant, since low values were observed in the cell-free medium (27.50 dyne/cm, indicating that biosurfactant was produced. The biosurfactant emulsified different hydrophobic sources and showed stability in the face of salinity, exposure to high temperatures and extreme pH conditions. These physiochemical properties demonstrate the potential for using biosurfactants produced by P. aeruginosa MSIC02 in various applications.

  7. Production of a new glycolipid biosurfactant from marine Nocardiopsis lucentensis MSA04 in solid-state cultivation.

    Science.gov (United States)

    Kiran, G Seghal; Thomas, T Anto; Selvin, Joseph

    2010-06-15

    Considering the need of potential biosurfactant producers and economic production processes using industrial waste, the present study aims to develop solid-state culture (SSC) of a marine actinobacterium for biosurfactant production. A potential biosurfactant producer Nocardiopsis lucentensis MSA04 was isolated from the marine sponge Dendrilla nigra. Among the substrates screened, wheat bran increased the production significantly (E(24) 25%) followed by oil seed cake and industrial waste such as tannery pretreated sludge, treated molasses (distillery waste) and pretreated molasses. Enhanced biosurfactant production was achieved under SSC conditions using kerosene as carbon source, beef extract as nitrogen source and wheat bran as substrate. The maximum production of biosurfactant by MSA04 occurred at a C/N ratio of 0.5 envisaging that a higher amount of nitrogen source is required by the strain compared to that of the carbon source. The kerosene and beef extract interactively increase the production and a stable production was attained with the influence of both factors independently. A significant interactive influence of secondary control factors such as copper sulfate and inoculum size was validated in response surface methods-based experiments. The surface active compound produced by MSA04 was characterized as glycolipid with a hydrophobic non-polar hydrocarbon chain (nonanoic acid methyl ester) and hydrophilic sugar, 3-acetyl 2,5 dimethyl furan. In conclusion, the strain N. lucentensis MSA04 was a potential source of glycolipid biosurfactant, could be used for the development of bioremediation processes in the marine environment. Copyright 2010 Elsevier B.V. All rights reserved.

  8. Biosurfactant production by Pseudomonas aeruginosain kefir and fish meal.

    Science.gov (United States)

    Kaskatepe, Banu; Yildiz, Sulhiye; Gumustas, Mehmet; Ozkan, Sibel A

    2015-01-01

    The aim of this study was to increase rhamnolipid production by formulating media using kefir and fish meal for Pseudomonas aeruginosa strains isolated from different environmental resources. The strains, named as H1, SY1, and ST1, capable of rhamnolipid production were isolated from soil contaminated with wastes originating from olive and fish oil factories. Additionally, P. aeruginosa ATCC 9027 strain, which is known as rhamnolipid producer, was included in the study. Initially, rhamnolipid production by the strains was determined in Mineral Salt Medium (MSM) and then in media prepared by using kefir and fish meal. The obtained rhamnolipids were purified and quantified according to Dubois et al. (1956). The quantity of rhamnolipids of ATCC, H1 and SY1 strains in kefir media were determined as 11.7 g/L, 10.8 g/L and 3.2 g/L, respectively, and in fish meal media as 12.3 g/L, 9.3 g/L and 10.3 g/L, respectively. In addition, effect of UV light exposure on rhamnolipid production was also investigated but contrary a decrease was observed. The results indicate that P. aeruginosa strains isolated from various environmental resources used in this study can be important due to their rhamnolipid yield, and fish meal, which is obtained from waste of fish, can be an alternative source in low cost rhamnolipid production.

  9. Biosurfactant production by Pseudomonas aeruginosain kefir and fish meal

    Directory of Open Access Journals (Sweden)

    Banu Kaskatepe

    2015-09-01

    Full Text Available The aim of this study was to increase rhamnolipid production by formulating media using kefir and fish meal for Pseudomonas aeruginosa strains isolated from different environmental resources. The strains, named as H1, SY1, and ST1, capable of rhamnolipid production were isolated from soil contaminated with wastes originating from olive and fish oil factories. Additionally, P. aeruginosa ATCC 9027 strain, which is known as rhamnolipid producer, was included in the study. Initially, rhamnolipid production by the strains was determined in Mineral Salt Medium (MSM and then in media prepared by using kefir and fish meal. The obtained rhamnolipids were purified and quantified according to Dubois et al. (1956. The quantity of rhamnolipids of ATCC, H1 and SY1 strains in kefir media were determined as 11.7 g/L, 10.8 g/L and 3.2 g/L, respectively, and in fish meal media as 12.3 g/L, 9.3 g/L and 10.3 g/L, respectively. In addition, effect of UV light exposure on rhamnolipid production was also investigated but contrary a decrease was observed. The results indicate that P. aeruginosa strains isolated from various environmental resources used in this study can be important due to their rhamnolipid yield, and fish meal, which is obtained from waste of fish, can be an alternative source in low cost rhamnolipid production.

  10. Production of biosurfactant on crude date syrup under saline conditions by entrapped cells of Natrialba sp. strain E21, an extremely halophilic bacterium isolated from a solar saltern (Ain Salah, Algeria).

    Science.gov (United States)

    Kebbouche-Gana, Salima; Gana, Mohamed Lamine; Ferrioune, Imen; Khemili, Souad; Lenchi, Nesrine; Akmouci-Toumi, Sihem; Bouanane-Darenfed, Nabila Amel; Djelali, Nacer-Eddine

    2013-11-01

    A bacterial strain E21 was isolated from a sample of water collected in the salt lake located close to Ain Salah, Algeria. The analysis of 16S rRNA gene sequence had indicated that the strain had 93 % sequence similarity with the genus Natrialba sp. strain E21 (GenBank, FR750525.1) and was considered extremely halophilic. Production of biosurfactant by the strain E21 with free and entrapped cells was investigated using soluble starch in the saline conditions. Biosurfactant synthesis was followed by measuring the surface tension and emulsifying index 9 days under optimal conditions (40 °C, pH 7). Some diffusional limitations in alginate and agar beads affected the kinetics of biosurfactant production when compared to that obtained with free cells culture. The minimum values of surface tension were 27 and 30 mN m(-1) achieved after 9 days with free and immobilized cells, respectively, while the corresponding maximum E24 values were 65.3 and 62.3 %, respectively. The re-use of bacterial cells along with the limited cell losses provided by the immobilized system might lead to significant reduction of the biosurfactant production cost.

  11. Biosurfactants for Microbubble Preparation and Application

    OpenAIRE

    Takeo Shiina; Zengshe Liu; Mitsutoshi Nakajima; Qingyi Xu

    2011-01-01

    Biosurfactants can be classified by their chemical composition and their origin. This review briefly describes various classes of biosurfactants based on their origin and introduces a few of the most widely used biosurfactants. The current status and future trends in biosurfactant production are discussed, with an emphasis on those derived from plants. Following a brief introduction of the properties of microbubbles, recent progress in the application of microbubble technology to molecular im...

  12. Avaliação cinética da produção de biossurfactantes bacterianos Bacteria biosurfactants production kinetic evaluation

    Directory of Open Access Journals (Sweden)

    Marta Heidtmann Pinto

    2009-01-01

    Full Text Available Biosurfactants present advantages in relation to the synthetic surfactants, as the biodegradability and low toxicity, and can be applied in the food industry, in pharmaceutical products, cosmetics and in the petroleum recovery. This paper aimed at selecting bacteria for biosurfactant production, evaluating the surface tension and the emulsifying activity and studying the fermentation process kinetics. The pure culture of Corynebacterium aquaticum showed capacity to promote emulsions formation and presented the smallest surface tension (28.8 mN m-1, and, in general, larger kinetic parameters, being selected as biosurfactant producer.

  13. Biofilm disruption potential of a glycolipid biosurfactant from marine Brevibacterium casei.

    Science.gov (United States)

    Kiran, George Seghal; Sabarathnam, Balu; Selvin, Joseph

    2010-08-01

    The antibiofilm activity of a glycolipid biosurfactant isolated from the marine actinobacterium Brevibacterium casei MSA19 was evaluated against pathogenic biofilms in vitro. The isolate B. casei MSA19 was a potential biosurfactant producer among the 57 stable strains isolated from the marine sponge Dendrilla nigra. The biosurfactant production was optimized under submerged fermentation. The purified glycolipid showed a broad spectrum of antimicrobial activity. Based on the minimum inhibitory concentration/minimum bactericidal concentration ratio, the glycolipid was determined as bacteriostatic. The glycolipid biosurfactant disrupted the biofilm formation under dynamic conditions. The disruption of the biofilm by the MSA19 glycolipid was consistent against mixed pathogenic biofilm bacteria. Therefore, the glycolipid biosurfactant can be used as a lead compound for the development of novel antibiofilm agents.

  14. Production of a Lipopeptide Biosurfactant by a Novel Bacillus sp. and Its Applicability to Enhanced Oil Recovery.

    Science.gov (United States)

    Varadavenkatesan, Thivaharan; Murty, Vytla Ramachandra

    2013-01-01

    Biosurfactants are surface-active compounds derived from varied microbial sources including bacteria and fungi. They are secreted extracellularly and have a wide range of exciting properties for bioremediation purposes. They also have vast applications in the food and medicine industry. With an objective of isolating microorganisms for enhanced oil recovery (EOR) operations, the study involved screening of organisms from an oil-contaminated site. Morphological, biochemical, and 16S rRNA analysis of the most promising candidate revealed it to be Bacillus siamensis, which has been associated with biosurfactant production, for the first time. Initial fermentation studies using mineral salt medium supplemented with crude oil resulted in a maximum biosurfactant yield of 0.64 g/L and reduction of surface tension to 36.1 mN/m at 96 h. Characterization studies were done using thin layer chromatography and Fourier transform infrared spectroscopy. FTIR spectra indicated the presence of carbonyl groups, alkyl bonds, and C-H and N-H stretching vibrations, typical of peptides. The extracted biosurfactant was stable at extreme temperatures, pH, and salinity. Its applicability to EOR was further verified by conducting sand pack column studies that yielded up to 60% oil recovery.

  15. Comparison of biosurfactant detection methods reveals hydrophobic surfactants and contact-regulated production

    Science.gov (United States)

    Biosurfactants are diverse molecules with numerous biological functions and industrial applications. A variety of environments were examined for biosurfactant-producing bacteria using a versatile new screening method. The utility of an atomized oil assay was assessed for a large number of bacteria...

  16. Biosurfactant production by Pseudomonas sp. and its role in aqueous phase partitioning and biodegradation of chlorpyrifos.

    Science.gov (United States)

    Singh, P B; Sharma, S; Saini, H S; Chadha, B S

    2009-09-01

    To study the effect of biosurfactant on aqueous phase solubility and biodegradation of chlorpyrifos. A Pseudomonas sp. (ChlD), isolated from agricultural soil by enrichment culture technique in the presence of chlorpyrifos, was capable of producing biosurfactant (rhamnolipids) and degrading chlorpyrifos (0.01 g l(-1)). The partially purified rhamnolipid biosurfactant preparation, having a CMC of 0.2 g l(-1), was evaluated for its ability to enhance aqueous phase partitioning and degradation of chlorpyrifos (0.01 g l(-1)) by ChlD strain. The best degradation efficiency was observed at 0.1 g l(-1) supplement of biosurfactant, as validated by GC and HPLC studies. The addition of biosurfactant at 0.1 g l(-1) resulted in more than 98% degradation of chlorpyrifos when compared to 84% in the absence of biosurfactant after 120-h incubation. This first report, to the best of our knowledge, on enhanced degradation of chlorpyrifos in the presence of biosurfactant(s), would help in developing bioremediation protocols to counter accumulation of organophosphates to toxic/carcinogenic levels in environment.

  17. Improved production, purification, and characterization of biosurfactants produced by Serratia marcescens SM3 and its isogenic SMRG-5 strain.

    Science.gov (United States)

    Rosas-Galván, Nashbly Sarela; Martínez-Morales, Fernando; Marquina-Bahena, Silvia; Tinoco-Valencia, Raunel; Serrano-Carreón, Leobardo; Bertrand, Brandt; León-Rodríguez, Renato; Guzmán-Aparicio, Josefina; Alvaréz-Berber, Laura; Trejo-Hernández, María R

    2018-02-19

    In this study, the biosurfactants (Bs) production of two Serratia marcescens strains (SM3 and its isogenic SMRG-5 strain) was improved and the tenso-active agents were purified and characterized. A 2 3 factorial design was used to evaluate the effect of nitrogen and carbon sources on the surface tension (ST) reduction and emulsion index (EI 24 ) of the produced Bs. Optimum Bs production by SM3 was achieved at high concentrations of carbon and nitrogen, reducing ST to 26.5 ± 0.28 dynes/cm, with an EI 24 of 79.9 ± 0.2%. Meanwhile, the best results for SMRG-5 were obtained at low concentrations, reducing the ST to 25.2 ± 0.2 dynes/cm, with an EI 24 of 89.7 ± 0.28%. The optimal conditions for Bs production were scaled up in a 2-L reactor, yielding 4.8 and 5.2 g/L for SM3 and SMRG-5, respectively. Gas Chromatography-Mass Spectrometry (GC-MS) analysis revealed the presence of two different lipopeptides (hidrofobic fractions: octadecanoic and hexadecanoic acid for SM3 and SMRG5, respectively). Both strains were capable of benzo [a] pyrene removal (59% after 72 H of culture). © 2018 International Union of Biochemistry and Molecular Biology, Inc.

  18. Isolation of basidiomycetous yeast Pseudozyma tsukubaensis and production of glycolipid biosurfactant, a diastereomer type of mannosylerythritol lipid-B.

    Science.gov (United States)

    Morita, Tomotake; Takashima, Masako; Fukuoka, Tokuma; Konishi, Masaaki; Imura, Tomohiro; Kitamoto, Dai

    2010-10-01

    The producers of glycolipid biosurfactant, mannosylerythritol lipid-B (MEL-B), were isolated from leaves of Perilla frutescens on Ibaraki in Japan. Four isolates, 1D9, 1D10, 1D11, and 1E5, were identified as basidiomycetous yeast Pseudozyma tsukubaensis by rDNA sequence and biochemical properties. The structure of MEL-B produced by these strains was analyzed by (1)H nuclear magnetic resonance and gas chromatography-mass spectrometry methods, and was determined to be the same as the diastereomer MEL-B produced by P. tsukubaensis NBRC 1940. Of these isolates, P. tsukubaensis 1E5 (JCM 16987) is capable of producing the largest amount of the diastereomer MEL-B from vegetable oils. In order to progress the diastereomer MEL-B production by strain 1E5, factors affecting the production, such as carbon and organic nutrient sources, were further examined. Olive oil and yeast extract were the best carbon and nutrient sources, respectively. Under the optimal conditions, a maximum yield, productivity, and yield coefficient of 73.1 g/L, 10.4 g L(-1) day(-1), and 43.5 g/g were achieved by feeding of olive oil in a 5-L jar-fermenter culture using strain 1E5.

  19. Auto-production of biosurfactants reverses the coffee ring effect in a bacterial system

    Science.gov (United States)

    Sempels, Wouter; de Dier, Raf; Mizuno, Hideaki; Hofkens, Johan; Vermant, Jan

    2013-04-01

    The deposition of material at the edge of evaporating droplets, known as the ‘coffee ring effect’, is caused by a radially outward capillary flow. This phenomenon is common to a wide array of systems including colloidal and bacterial systems. The role of surfactants in counteracting these coffee ring depositions is related to the occurrence of local vortices known as Marangoni eddies. Here we show that these swirling flows are universal, and not only lead to a uniform deposition of colloids but also occur in living bacterial systems. Experiments on Pseudomonas aeruginosa suggest that the auto-production of biosurfactants has an essential role in creating a homogeneous deposition of the bacteria upon drying. Moreover, at biologically relevant conditions, intricate time-dependent flows are observed in addition to the vortex regime, which are also effective in reversing the coffee ring effect at even lower surfactant concentrations.

  20. Utilization of response surface for optimization of the production of a biosurfactant with application in the removal of petroleum-derived; Utilizacao de superficie de resposta para a otimizacao da producao de um biossurfactante com aplicacao na remocao de derivado de petroleo

    Energy Technology Data Exchange (ETDEWEB)

    Sarubbo, Leonie A.; Rufino, Raquel D.; Luna, Juliana M.; Farias, Charles B.B.; Santos, Valdemir A. dos [Universidade Catolica de Pernambuco (UNICAP), Recife, PE (Brazil)

    2012-07-01

    The surfactants, amphipathic compounds capable of reducing the surface tension of aqueous media, find application in many industries, especially in the petroleum, cosmetic and food, such as dispersants, emulsifiers and surfactants. With the necessity of environmental preservation, surfactants of micro-organisms origin, in substitution of synthetic surfactants, have become very attractive, since they are biodegradable and less toxic although their production costs are still high because of the substrates used and of the purification processes involved. Considering the need to reduce costs associated with the production of microbial surfactants, a strain of bacteria of the genus Pseudomonas, isolated from the port area was cultured in medium containing low-cost agro-industrial wastes, molasses and corn steep liquor, as substrates according a central composite rotatable design (CCRD) in order to evaluate the influence of independent variables molasses and corn steep liquor concentrations on the response variable surface tension. The biosurfactant was able to reduce the water surface tension from 71 mN / m to values around 27.5 mN / m. The dispersion ability and the capacity of oil removal of the surfactant was demonstrated. The possibility of application of biosurfactants in the remediation of oil polluted environments motivates the advancement of research to develop this technology for effective use in treatment of contaminated soils and waters. (author)

  1. In situ biosurfactant production and hydrocarbon removal by Pseudomonas putida CB-100 in bioaugmented and biostimulated oil-contaminated soil

    Directory of Open Access Journals (Sweden)

    Martínez-Toledo Ángeles

    2013-01-01

    Full Text Available In situ biosurfactant (rhamnolipid production by Pseudomonas putida CB-100 was achieved during a bioaugmented and biostimulated treatment to remove hydrocarbons from aged contaminated soil from oil well drilling operations. Rhamnolipid production and contaminant removal were determined for several treatments of irradiated and non-irradiated soils: nutrient addition (nitrogen and phosphorus, P. putida addition, and addition of both (P. putida and nutrients. The results were compared against a control treatment that consisted of adding only sterilized water to the soils. In treatment with native microorganisms (non-irradiated soils supplemented with P. putida, the removal of total petroleum hydrocarbons (TPH was 40.6%, the rhamnolipid production was 1.54 mg/kg, and a surface tension of 64 mN/m was observed as well as a negative correlation (R = -0.54; p < 0.019 between TPH concentration (mg/kg and surface tension (mN/m, When both bacteria and nutrients were involved, TPH levels were lowered to 33.7%, and biosurfactant production and surface tension were 2.03 mg/kg and 67.3 mN/m, respectively. In irradiated soil treated with P. putida, TPH removal was 24.5% with rhamnolipid generation of 1.79 mg/kg and 65.6 mN/m of surface tension, and a correlation between bacterial growth and biosurfactant production (R = -0.64; p < 0.009 was observed. When the nutrients and P. putida were added, TPH removal was 61.1%, 1.85 mg/kg of biosurfactants were produced, and the surface tension was 55.6 mN/m. In summary, in irradiated and non-irradiated soils, in situ rhamnolipid production by P. putida enhanced TPH decontamination of the soil.

  2. Effective Biosurfactants Production by Pseudomonas aeruginosa and its Efficacy on Different Oils

    Directory of Open Access Journals (Sweden)

    Sarita Kumari

    2010-07-01

    Full Text Available A rhamnolipid producing bacterium, Pseudomonas aeruginosa was isolated from contaminated soil with oily wastes. The Pseudomonas aeruginosa grown with glucose and corn oil as a carbon source produced bio-surfactant. This biosurfactant was purified by procedures that included chloroform-ethanol extraction and 0.05M bicarbonate treatments. The active compound was identified as rhamnolipid by using thin layer chromatography. The emulsification activity of bio-surfactant, the coconut oil responded better than the olive oil, groundnut oil and sunflower oil and gave a maximum level of 1 cm.

  3. Isolation and Characterization of Biosurfactant Producing Bacteria for the Application in Enhanced Oil Recovery

    Science.gov (United States)

    Prasad, Niraj; Dasgupta, Sumita; Chakraborty, Mousumi; Gupta, Smita

    2017-07-01

    In the present study, a biosurfactant producing bacterial strain was isolated, screened and identified. Further, various fermentation conditions (such as pH (5-10), incubation period (24-96h) and incubation temperature (20-60 °C) were optimized for maximum production of biosurfactant. The produced biosurfactant was characterized by measuring emulsification index, foaming characteristics, rhamnolipid detection, interfacial tension between water and oil and stability against pH and temperature for its potential application in oil recovery process. The additional oil recovery for two different sand, sand1 and sand2, was found to be 49% and 38%, respectively.

  4. Biosurfactant production from marine hydrocarbon-degrading consortia and pure bacterial strains using crude oil as carbon source.

    Science.gov (United States)

    Antoniou, Eleftheria; Fodelianakis, Stilianos; Korkakaki, Emmanouela; Kalogerakis, Nicolas

    2015-01-01

    Biosurfactants (BSs) are "green" amphiphilic molecules produced by microorganisms during biodegradation, increasing the bioavailability of organic pollutants. In this work, the BS production yield of marine hydrocarbon degraders isolated from Elefsina bay in Eastern Mediterranean Sea has been investigated. The drop collapse test was used as a preliminary screening test to confirm BS producing strains or mixed consortia. The community structure of the best consortia based on the drop collapse test was determined by 16S-rDNA pyrotag screening. Subsequently, the effect of incubation time, temperature, substrate and supplementation with inorganic nutrients, on BS production, was examined. Two types of BS - lipid mixtures were extracted from the culture broth; the low molecular weight BS Rhamnolipids and Sophorolipids. Crude extracts were purified by silica gel column chromatography and then identified by thin layer chromatography and Fourier transform infrared spectroscopy. Results indicate that BS production yield remains constant and low while it is independent of the total culture biomass, carbon source, and temperature. A constant BS concentration in a culture broth with continuous degradation of crude oil (CO) implies that the BS producing microbes generate no more than the required amount of BSs that enables biodegradation of the CO. Isolated pure strains were found to have higher specific production yields than the complex microbial marine community-consortia. The heavy oil fraction of CO has emerged as a promising substrate for BS production (by marine BS producers) with fewer impurities in the final product. Furthermore, a particular strain isolated from sediments, Paracoccus marcusii, may be an optimal choice for bioremediation purposes as its biomass remains trapped in the hydrocarbon phase, not suffering from potential dilution effects by sea currents.

  5. Biosurfactant production from marine hydrocarbon-degrading consortia and pure bacterial strains using crude oil as carbon source

    Science.gov (United States)

    Antoniou, Eleftheria; Fodelianakis, Stilianos; Korkakaki, Emmanouela; Kalogerakis, Nicolas

    2015-01-01

    Biosurfactants (BSs) are “green” amphiphilic molecules produced by microorganisms during biodegradation, increasing the bioavailability of organic pollutants. In this work, the BS production yield of marine hydrocarbon degraders isolated from Elefsina bay in Eastern Mediterranean Sea has been investigated. The drop collapse test was used as a preliminary screening test to confirm BS producing strains or mixed consortia. The community structure of the best consortia based on the drop collapse test was determined by 16S-rDNA pyrotag screening. Subsequently, the effect of incubation time, temperature, substrate and supplementation with inorganic nutrients, on BS production, was examined. Two types of BS – lipid mixtures were extracted from the culture broth; the low molecular weight BS Rhamnolipids and Sophorolipids. Crude extracts were purified by silica gel column chromatography and then identified by thin layer chromatography and Fourier transform infrared spectroscopy. Results indicate that BS production yield remains constant and low while it is independent of the total culture biomass, carbon source, and temperature. A constant BS concentration in a culture broth with continuous degradation of crude oil (CO) implies that the BS producing microbes generate no more than the required amount of BSs that enables biodegradation of the CO. Isolated pure strains were found to have higher specific production yields than the complex microbial marine community-consortia. The heavy oil fraction of CO has emerged as a promising substrate for BS production (by marine BS producers) with fewer impurities in the final product. Furthermore, a particular strain isolated from sediments, Paracoccus marcusii, may be an optimal choice for bioremediation purposes as its biomass remains trapped in the hydrocarbon phase, not suffering from potential dilution effects by sea currents. PMID:25904907

  6. Isolation and characterization of biosurfactant production under extreme environmental conditions by alkali-halo-thermophilic bacteria from Saudi Arabia.

    Science.gov (United States)

    Elazzazy, Ahmed M; Abdelmoneim, T S; Almaghrabi, O A

    2015-07-01

    Twenty three morphologically distinct microbial colonies were isolated from soil and sea water samples, which were collected from Jeddah region, Saudi Arabia for screening of the most potent biosurfactant strains. The isolated bacteria were selected by using different methods as drop collapse test, oil displacement test, blue agar test, blood hemolysis test, emulsification activity and surface tension. The results showed that the ability of Virgibacillus salarius to grow and reduce surface tension under a wide range of pH, salinities and temperatures gives bacteria isolate an advantage in many applications such as pharmaceutical, cosmetics, food industries and bioremediation in marine environment. The biosurfactant production by V. salarius decreased surface tension and emulsifying activity (30 mN/m and 80%, respectively). In addition to reducing the production cost of biosurfactants by tested several plant-derived oils such as jatropha oil, castor oils, jojoba oil, canola oil and cottonseed oil. In this respect the feasibility to reusing old frying oil of sunflower for production rhamnolipids and sophorolipids, their use that lead to solve many ecological and industrial problems.

  7. Isolation and characterization of biosurfactant production under extreme environmental conditions by alkali-halo-thermophilic bacteria from Saudi Arabia

    Science.gov (United States)

    Elazzazy, Ahmed M.; Abdelmoneim, T.S.; Almaghrabi, O.A.

    2014-01-01

    Twenty three morphologically distinct microbial colonies were isolated from soil and sea water samples, which were collected from Jeddah region, Saudi Arabia for screening of the most potent biosurfactant strains. The isolated bacteria were selected by using different methods as drop collapse test, oil displacement test, blue agar test, blood hemolysis test, emulsification activity and surface tension. The results showed that the ability of Virgibacillus salarius to grow and reduce surface tension under a wide range of pH, salinities and temperatures gives bacteria isolate an advantage in many applications such as pharmaceutical, cosmetics, food industries and bioremediation in marine environment. The biosurfactant production by V. salarius decreased surface tension and emulsifying activity (30 mN/m and 80%, respectively). In addition to reducing the production cost of biosurfactants by tested several plant-derived oils such as jatropha oil, castor oils, jojoba oil, canola oil and cottonseed oil. In this respect the feasibility to reusing old frying oil of sunflower for production rhamnolipids and sophorolipids, their use that lead to solve many ecological and industrial problems. PMID:26150754

  8. Production of biosurfactants from Pseudomonas aeruginosa PA 1 isolated in oil environments

    Directory of Open Access Journals (Sweden)

    L.M. Santa Anna

    2002-04-01

    Full Text Available The potential production of rhamnolipid-type biosurfactants is assessed based on the development of a fermentative process with a strain of Pseudomonas aeruginosa PA1, which was isolated from oil production wastewater in the Northeast of Brazil. These production of molecules using different carbon (n-hexadecane, paraffinic oil, glycerol and babassu oil and nitrogen sources (NaNO3, (NH42SO4 and CH4N2O was studied. The best results were obtained when using glycerol as substrate. A C/N ratio of 60/1 and use of sodium nitrate as nitrogen source resulted in higher production of the rhamnolipid, expressed by rhamnose (3.16 g/L and by the yield in relation to biomass (Yp/x = 0.70 g/g. Additionally, physical-chemical characteristics of the spent broth with and without cells were studied, providing a low critical micelle concentration of 19 mg/L and toxicity values of 13 and 13.8 mg/L using two test organisms, the micro crustacean Daphnia similis and the bacterium Vibrio fisheri (Microtox, respectively.

  9. Effects of biosurfactant production by indigenous soil microorganisms on bioremediation of a co-contaminated soil in batch experiments

    Energy Technology Data Exchange (ETDEWEB)

    Jalali, F.; Mulligan, C.N. [Concordia Univ., Centre for Building Studies, Montreal, PQ (Canada). Dept. of Building, Civil and Environmental Engineering

    2007-07-01

    The challenge of remediating soils that are contaminated with both hydrocarbon compounds and metals was discussed, with particular reference to an in-situ bioremediation technique that was developed in the 1970s to deal with contaminated soils. The technique involves a two-stage process where water with added oxygen and nutrients is applied onto and injected into a contaminated area to stimulate the indigenous microbial populations in the soil. In addition to using organic pollutants as their carbon source, microorganisms can facilitate the removal of metals from the soil matrix and attenuate the toxicity of certain metals. Extraction wells placed downstream of the contaminated soils are used to remove and treat the water to eliminate any mobilized contaminants. This paper presented the results of batch experiments that evaluated the feasibility of biosurfactant production for the purpose of bioremediating a soil contaminated with aged petroleum hydrocarbons and heavy metals. The first phase of the study examined the growth of the native microbial population and the biodegradation of petroleum hydrocarbons, the production of biosurfactant and the mobilization of the total petroleum hydrocarbons (TPH) and metals into the aqueous phase. Biodegradation of petroleum hydrocarbons was observed in both soil and soil amended with nitrogen and phosphorous. However, the nutrient-amended soil had higher biodegradation of petroleum hydrocarbons, where 36 per cent of TPH was degraded by the end of the 50 day experiment, compared to 15 per cent for the non-amended soils. The concentration of biosurfactants in the same period increased 3 times their critical micelle concentration. It was concluded that biosurfactant production enhances the bioremediation of co-contaminated soils. 36 refs., 1 tab., 8 figs.

  10. Biosurfactant production from Pseudomonas taiwanensis L1011 and its application in accelerating the chemical and biological decolorization of azo dyes.

    Science.gov (United States)

    Liu, Cong; You, Yanting; Zhao, Ruofei; Sun, Di; Zhang, Peng; Jiang, Jihong; Zhu, Aihua; Liu, Weijie

    2017-11-01

    Dye dispersion and the interaction efficiency between azoreductases and dye molecules are rate-limiting steps for the decolorization of azo dyes. In this study, a biosurfactant-producing strain, Pseudomonas taiwanensis L1011, was isolated from crude oil. To increase the yield of the biosurfactant BS-L1011 from P. taiwanensis L1011, culture conditions were optimized including temperature, initial pH, carbon source, nitrogen source and C/N ratio. A maximum yield of 1.12g/L of BS-L1011 was obtained using D-mannitol as carbon source and yeast extract/urea as compound nitrogen source with C/N ratio of 10/4, pH 7.0 and 28°C. BS-L1011 exhibited a low critical micelle concentration (CMC) of 10.5mg/L and was able to reduce the surface tension of water to 25.8±0.1 mN/m. BS-L1011 was stable over a wide range of temperatures, pH values and salt concentrations. The biosurfactant is reported for the first time to accelerate chemical decolorization of Congo red by sodium hypochlorite, and biological decolorization of Amaranth by Bacillus circulans BWL1061, thus showing a potential in the treatment of dyeing wastewater. Copyright © 2017 Elsevier Inc. All rights reserved.

  11. Screening for biosurfactant production by 2,4,6-trinitrotoluene-transforming bacteria.

    Science.gov (United States)

    Avila-Arias, H; Avellaneda, H; Garzón, V; Rodríguez, G; Arbeli, Z; Garcia-Bonilla, E; Villegas-Plazas, M; Roldan, F

    2017-08-01

    To isolate and identify TNT-transforming cultures from explosive-contaminated soils with the ability to produce biosurfactants. Bacteria (pure and mixed cultures) were selected based on their ability to transform TNT in minimum media with TNT as the sole nitrogen source and an additional carbon source. TNT-transforming bacteria were identified by 16S rRNA gene sequencing. TNT transformation rates were significantly lower when no additional carbon or nitrogen sources were added. Surfactant production was enabled by the presence of TNT. Fourteen cultures were able to transform the explosive (>50%); of these, five showed a high transformation capacity (>90%), and six produced surfactants. All explosive-transforming cultures contained Proteobacteria of the genera Achromobacter, Stenotrophomonas, Pseudomonas, Sphingobium, Raoultella, Rhizobium and Methylopila. These cultures transformed TNT when an additional carbon source was added. Remarkably, Achromobacter spanius S17 and Pseudomonas veronii S94 have high TNT transformation rates and are surfactant producers. TNT is a highly toxic, mutagenic and carcinogenic nitroaromatic explosive; therefore, bioremediation to eliminate or mitigate its presence in the environment is essential. TNT-transforming cultures that produce surfactants are a promising method for remediation. To the best of our knowledge, this is the first report that links surfactant production and TNT transformation by bacteria. © 2017 The Society for Applied Microbiology.

  12. Isolation and identification of biosurfactant-producing strains from the genus Pseudomonas aeruginosa and antibacterial effects of biosurfactant production in vitro

    Directory of Open Access Journals (Sweden)

    Salman Ahmady-Asbchin

    2013-01-01

    Full Text Available Introduction: Biosurfactants are amphiphilic biological compounds produced extracellularly or as part of the cell membranes by a variety of microorganisms. Because of their use in various industries, they are of a particular importance. The aim of this study was to identify a strain of bacteria of the genus Pseudomonas aeruginosa biosurfactant producers. Materials and methods: In this study, different samples of oil, water and soil contaminated with oil were prepared. Hemolytic activity, emulsification activity and measurement of surface tension were used and selected strains were identified by biochemical tests. The nature and effect of antibacterial biosurfactant was evaluated for strain selection.Results: In this study, eighty eight bacterial strains were isolated. Twenty four strains were isolated from the isolated strains with hemolytic activity. Among which, 14 strains have emulsification activity more than 70% and at last four strains reached surface tension to be less than 40 mN/m. Selected strain based on biochemical tests was recognized as a Pseudomonas aeruginosa. The nature of biosurfactant was determined by TLC, and proved to be of glycolipid kind. Therefore, the produced biosurfactant of the selected strain had antibacterial activity against six bacterial infectious. Sensitive bacteria to the effects of biosurfactant extract of Pseudomonas aeruginosa83, was Staphylococcus aureus and the most resistant bacteria to these extract, was the Proteus mirabilis. The results of MIC, MBC showed that MIC of the extract in concentration of 63 and 125 mg/ml on Escherichia coli, Staphylococcus epidermidis and Staphylococcus aureus respectively. Also, the MBC were extract in concentration of 63 and 125mg/ml on Staphylococcus epidermidis and Staphylococcus aureus respectively.Discussion and conclusion: Pseudomonas aeruginosa had high potential in reducing the surface tension and biosurfactant extracted had high antibacterial effects. Therefore, it

  13. Production of biosurfactants for application in the removal of environmental contaminants generated in the petroleum industry; Producao de biossurfactantes para aplicacao na remocao de contaminantes ambientais gerados na industria do petroleo

    Energy Technology Data Exchange (ETDEWEB)

    Sarubbo, Leonie A.; Rufino, Raquel D.; Luna, Juliana M. de; Farias, Charles B.B.; Santos, Valdemir A. dos [Universidade Catolica de Pernambuco (UNICAP), Recife, PE (Brazil)

    2012-07-01

    This paper describes the application of microbial surfactants in removing crude oil and marine environment located in the proximity of the Pernambuco Thermoelectric. Two biosurfactants were produced by yeasts Candida sphaerica and C. lipolytica grown in industrial wastes during 72 and 144 hours, respectively. The surface tensions of the biomolecules (25 mN/m) were determined, the production yields were estimated (8 and 9 g/L) and the CMC determined (0.03%). The biosurfactants were applied to samples of sea water and rocks contaminated with petroleum and motor oil. The results demonstrated the oil dispersant action of the biosurfactant from C. sphaerica and the emulsifying ability of the biosurfactant from C. lipolytica. Percentages removals of 100% of oil and petroleum were obtained for both biosurfactants. The possibility of application of biosurfactants in the remediation of oil polluted environments motivates the advancement of research to develop this alternative technology for effective use in the Termope Thermoelectric treatment systems. (author)

  14. Biosurfactant production by halotolerant Rhodococcus fascians from Casey Station, Wilkes Land, Antarctica.

    Science.gov (United States)

    Gesheva, Victoria; Stackebrandt, Erko; Vasileva-Tonkova, Evgenia

    2010-08-01

    Isolate A-3 from Antarctic soil in Casey Station, Wilkes Land, was characterized for growth on hydrocarbons. Use of glucose or kerosene as a sole carbon source in the culture medium favoured biosynthesis of surfactant which, by thin-layer chromatography, indicated the formation of a rhamnose-containing glycolipid. This compound lowered the surface tension at the air/water interface to 27 mN/m as well as inhibited the growth of B. subtilis ATCC 6633 and exhibited hemolytic activity. A highly hydrophobic surface of the cells suggests that uptake occurs via a direct cell-hydrocarbon substrate contact. Strain A-3 is Gram-positive, halotolerant, catalase positive, urease negative and has rod-coccus shape. Its cell walls contained meso-diaminopimelic acid. Phylogenetic analysis based on comparative analysis of 16S rRNA gene sequences revealed that strain A-3 is closely related to Rhodococcus fascians with which it shares 100% sequence similarity. This is the first report on rhamnose-containing biosurfactant production by Rhodococcus fascians isolated from Antarctic soil.

  15. Sulfur source-mediated transcriptional regulation of the rhlABC genes involved in biosurfactants production by Pseudomonas sp. strain AK6U.

    Science.gov (United States)

    Ismail, Wael; El Nayal, Ashraf M; Ramadan, Ahmed R; Abotalib, Nasser

    2014-01-01

    Despite the nutritional significance of sulfur, its influence on biosurfactants production has not been sufficiently studied. We investigated the expression of key biosurfactants production genes, rhlABC, in cultures of Pseudomonas sp. AK6U grown with inorganic or organic sulfur sources. AK6U grew with either inorganic sulfate (MgSO4), dibenzothiophene (DBT), or DBT-sulfone as a sole sulfur source in the presence of glucose as a carbon source. The AK6U cultures produced variable amounts of biosurfactants depending on the utilized sulfur source. Biosurfactants production profile of the DBT cultures was significantly different from that of the DBT-sulfone and inorganic sulfate cultures. The last two cultures were very similar in terms of biosurfactants productivity. Biosurfactants yield in the DBT cultures (1.3 g/L) was higher than that produced by the DBT-sulfone (0.5 g/L) and the inorganic sulfate (0.44 g/L) cultures. Moreover, the surface tension reduction in the DBT cultures (33 mN/m) was much stronger than that measured in the DBT-sulfone (58 mN/m) or inorganic sulfate (54 mN/m) cultures. RT-qPCR revealed variations in the expression levels of the rhlABC genes depending on the sulfur source. The DBT cultures had higher expression levels for the three genes as compared to the DBT-sulfone and inorganic sulfate cultures. There was no significant difference in the expression profiles between the DBT-sulfone and the MgSO4 cultures. The increased expression of rhlC in the DBT cultures is indicative for production of higher amounts of dirhamnolipids compared to the DBT-sulfone and inorganic sulfate cultures. The gene expression results were in good agreement with the biosurfactants production yields and surface tension measurements. The sulfur source mediates a fine-tuned mechanism of transcriptional regulation of biosurfactants production genes. Our findings can have an impact on industrial production of biosurfactants and other biotechnological processes like

  16. Biosurfactants in plant-Pseudomonas interactions and their importance to biocontrol.

    Science.gov (United States)

    D'aes, Jolien; De Maeyer, Katrien; Pauwelyn, Ellen; Höfte, Monica

    2010-06-01

    Production of biosurfactants is a common feature in bacteria, and in particular in plant-associated species. These bacteria include many plant beneficial and plant pathogenic Pseudomonas spp., which produce primarily cyclic lipopeptide and rhamnolipid type biosurfactants. Pseudomonas-derived biosurfactants are involved in many important bacterial functions. By modifying surface properties, biosurfactants can influence common traits such as surface motility, biofilm formation and colonization. Biosurfactants can alter the bio-availability of exogenous compounds, such as nutrients, to promote their uptake, and of endogenous metabolites, including phenazine antibiotics, resulting in an enhanced biological activity. Antibiotic activity of biosurfactants towards microbes could play a role in intraspecific competition, self-defence and pathogenesis. In addition, bacterial surfactants can affect plants in different ways, either protecting them from disease, or acting as a toxin in a plant-pathogen interaction. Biosurfactants are involved in the biocontrol activity of an increasing number of Pseudomonas strains. Consequently, further insight into the roles and activities of surfactants produced by bacteria could provide means to optimize the use of biological control as an alternative crop protection strategy. © 2009 Society for Applied Microbiology and Blackwell Publishing Ltd.

  17. Pseudomonas sp. BUP6, a novel isolate from Malabari goat produces an efficient rhamnolipid type biosurfactant.

    Science.gov (United States)

    Priji, Prakasan; Sajith, Sreedharan; Unni, Kizhakkepowathial Nair; Anderson, Robin C; Benjamin, Sailas

    2017-01-01

    This study describes the characteristics of a biosurfactant produced by Pseudomonas sp. BUP6, a rumen bacterium, and optimization of parameters required for its production. Initial screening of five parameters (pH, temperature, agitation, incubation, and substrate concentration) was carried out employing Plackett-Burman design, which reduced the number of parameters to 3 (pH, temperature, and incubation) according to their significance on the yield of biosurfactant. A suitable statistical model for the production of biosurfactant by Pseudomonas sp. BUP6 was established according to Box-Behnken design, which resulted in 11% increase (at pH 7, 35 °C, incubation 75 h) in the yield (2070 mg L -1 ) of biosurfactant. The biosurfactant was found stable at a wide range of pH (3-9) with 48 mg L -1 critical micelle concentration; and maintained over 90% of its emulsification ability even after boiling and in presence of sodium chloride (0.5%). The highest cell hydrophobicity (37%) and emulsification (69%) indices were determined with groundnut oil and kerosene, respectively. The biosurfactant was found to inhibit the growth and adhesion of E. coli and S. aureus significantly. From the phytotoxicity studies, the biosurfactant did not show any adverse effect on the germinating seeds of rice and green gram. The structural characterization of biosurfactant employing orcinol method, thin layer chromatography and FT-IR indicated that it is a rhamnolipid (glycolipid). Thus, Pseudomonas sp. BUP6, a novel isolate from Malabari goat is demonstrated as a producer of an efficient rhamnolipid type biosurfactant suitable for application in various industries. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. Production, Structural Elucidation, and In Vitro Antitumor Activity of Trehalose Lipid Biosurfactant from Nocardia farcinica Strain.

    Science.gov (United States)

    Christova, Nelly; Lang, Siegmund; Wray, Victor; Kaloyanov, Kaloyan; Konstantinov, Spiro; Stoineva, Ivanka

    2015-04-01

    The objective of this study was to isolate and identify the chemical structure of a biosurfactant produced by Nocardia farcinica strain BN26 isolated from soil, and evaluate its in vitro antitumor activity on a panel of human cancer cell lines. Strain BN26 was found to produce glycolipid biosurfactant on n-hexadecane as the sole carbon source. The biosurfactant was purified using medium-pressure liquid chromatography and characterized as trehalose lipid tetraester (THL) by nuclear magnetic resonance spectroscopy and mass spectrometry. Subsequently, the cytotoxic effects of THL on cancer cell lines BV-173, KE-37 (SKW-3), HL-60, HL-60/DOX, and JMSU-1 were evaluated by MTT assay. It was shown that THL exerted concentration-dependent antiproliferative activity against the human tumor cell lines and mediated cell death by the induction of partial oligonucleosomal DNA fragmentation. These findings suggest that THL could be of potential to apply in biomedicine as a therapeutic agent.

  19. Isolation and characterization of a biosurfactant-producing heavy metal resistant Rahnella sp. RM isolated from chromium-contaminated soil

    OpenAIRE

    GOVARTHANAN, Muthusamy; MYTHILI, R.; SELVANKUMAR, Thangasamy; KAMALA-KANNAN, S.; CHOI, DuBok; CHANG, Young-Cheol

    2017-01-01

    Objective of the study was to isolate heavy metal resistant bacteria from chromium-contaminated subsurface soil and investigate biosurfactant production and heavy metal bioremediation. Based on 16S rRNA gene sequence and phylogenetic analysis, the isolate was identified as Rahnella sp. RM. The biosurfactant production by heavy metal resistant Rahnella sp. RM was optimized using Box- Behnken design (BBD). The maximum emulsification activity was obtained 66% at 6% soybean meal in pH 7.0 and 33....

  20. First report of a lipopeptide biosurfactant from thermophilic bacterium Aneurinibacillus thermoaerophilus MK01 newly isolated from municipal landfill site.

    Science.gov (United States)

    Sharafi, Hakimeh; Abdoli, Mahya; Hajfarajollah, Hamidreza; Samie, Nima; Alidoust, Leila; Abbasi, Habib; Fooladi, Jamshid; Zahiri, Hossein Shahbani; Noghabi, Kambiz Akbari

    2014-07-01

    A biosurfactant-producing thermophile was isolated from the Kahrizak landfill of Tehran and identified as a bacterium belonging to the genus Aneurinibacillus. A thermostable lipopeptide-type biosurfactant was purified from the culture medium of this bacterium and showed stability in the temperature range of 20-90 °C and pH range of 5-10. The produced biosurfactant could reduce the surface tension of water from 72 to 43 mN/m with a CMC of 1.21 mg/mL. The strain growing at a temperature of 45 °C produces a substantial amount of 5 g/L of biosurfactant in the medium supplemented with sunflower oil as the sole carbon source. Response surface methodology was employed to optimize the biosurfactant production using sunflower oil, sodium nitrate, and yeast extract as variables. The optimization resulted in 6.75 g/L biosurfactant production, i.e., 35% improved as compared to the unoptimized condition. Thin-layer chromatography, FTIR spectroscopy, 1H-NMR spectroscopy, and biochemical composition analysis confirmed the lipopeptide structure of the biosurfactant.

  1. In situ biosurfactant production and hydrocarbon removal by Pseudomonas putida CB-100 in bioaugmented and biostimulated oil-contaminated soil.

    Science.gov (United States)

    Ángeles, Martínez-Toledo; Refugio, Rodríguez-Vázquez

    2013-01-01

    In situ biosurfactant (rhamnolipid) production by Pseudomonas putida CB-100 was achieved during a bioaugmented and biostimulated treatment to remove hydrocarbons from aged contaminated soil from oil well drilling operations. Rhamnolipid production and contaminant removal were determined for several treatments of irradiated and non-irradiated soils: nutrient addition (nitrogen and phosphorus), P. putida addition, and addition of both (P. putida and nutrients). The results were compared against a control treatment that consisted of adding only sterilized water to the soils. In treatment with native microorganisms (non-irradiated soils) supplemented with P. putida, the removal of total petroleum hydrocarbons (TPH) was 40.6%, the rhamnolipid production was 1.54 mg/kg, and a surface tension of 64 mN/m was observed as well as a negative correlation (R = -0.54; p soil treated with P. putida, TPH removal was 24.5% with rhamnolipid generation of 1.79 mg/kg and 65.6 mN/m of surface tension, and a correlation between bacterial growth and biosurfactant production (R = -0.64; p soils, in situ rhamnolipid production by P. putida enhanced TPH decontamination of the soil.

  2. Production of biosurfactants for environment remediation contaminated with oil and derivatives; Producao de biossurfactantes para remediacao de ambiente contaminados com petroleo e derivados

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Adriano Henrique Soares de; Guimaraes, Juliana Aguilar; Hiluy Filho, Joao Jose; Lopes, Ada Amelia Sanders [Universidade Federal do Ceara, Fortaleza, CE (Brazil). Dept. de Engenharia Quimica]. E-mail: hiluy@ufc.br

    2003-07-01

    Biosurfactants are emulsifiers of hydrocarbons produced by bacteria, yeast and fungi. They are polymers that form micelles and stay at the interface between liquid of different polarities. This work aim to study the development of the biosurfactant production process and its application related to oil sludge. This research has been done in three different steps: the Acinetobacter iwoffii inoculation, its growth in a nutritive broth and the fermentation under specific conditions. It was used three different kind of substrates: ethanol, glycerol and kerosene for comparison. The surfactant potential was evaluated by the emulsification indexes with suspension biomass and efficiency tests with oil sludge. Preliminary results show that biosurfactant production by Acinetobacter iwoffii can be a feasible process due to the satisfactory emulsification indexes that have been obtained. (author)

  3. Exploiting the aerobic endospore-forming bacterial diversity in saline and hypersaline environments for biosurfactant production.

    Science.gov (United States)

    de Almeida Couto, Camila Rattes; Alvarez, Vanessa Marques; Marques, Joana Montezano; de Azevedo Jurelevicius, Diogo; Seldin, Lucy

    2015-10-28

    Biosurfactants are surface-active biomolecules with great applicability in the food, pharmaceutical and oil industries. Endospore-forming bacteria, which survive for long periods in harsh environments, are described as biosurfactant producers. Although the ubiquity of endospore-forming bacteria in saline and hypersaline environments is well known, studies on the diversity of the endospore-forming and biosurfactant-producing bacterial genera/species in these habitats are underrepresented. In this study, the structure of endospore-forming bacterial communities in sediment/mud samples from Vermelha Lagoon, Massambaba, Dois Rios and Abraão Beaches (saline environments), as well as the Praia Seca salterns (hypersaline environments) was determined via denaturing gradient gel electrophoresis. Bacterial strains were isolated from these environmental samples and further identified using 16S rRNA gene sequencing. Strains presenting emulsification values higher than 30 % were grouped via BOX-PCR, and the culture supernatants of representative strains were subjected to high temperatures and to the presence of up to 20 % NaCl to test their emulsifying activities in these extreme conditions. Mass spectrometry analysis was used to demonstrate the presence of surfactin. A diverse endospore-forming bacterial community was observed in all environments. The 110 bacterial strains isolated from these environmental samples were molecularly identified as belonging to the genera Bacillus, Thalassobacillus, Halobacillus, Paenibacillus, Fictibacillus and Paenisporosarcina. Fifty-two strains showed emulsification values of at least 30%, and they were grouped into 18 BOX groups. The stability of the emulsification values varied when the culture supernatants of representative strains were subjected to high temperatures and to the presence of up to 20% NaCl. The presence of surfactin was demonstrated in one of the most promising strains. The environments studied can harbor endospore

  4. Biosurfactant-enhanced hydrogen production from organic fraction of municipal solid waste using co-culture of E. coli and Enterobacter aerogenes.

    Science.gov (United States)

    Sharma, Preeti; Melkania, Uma

    2017-11-01

    The effect of biosurfactants (surfactin and saponin) on the hydrogen production from organic fraction of municipal solid waste (OFMSW) was investigated using co-culture of facultative anaerobes Enterobacter aerogenes and E. coli. The biosurfactants were applied in the concentration ranges of 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5 and 5.0% each. Cumulative hydrogen production (P), maximum hydrogen production rate (Rmax) and lag phases (λ) were analyzed using modified Gompertz model. Results revealed that both the biosurfactants were effective in hydrogen production enhancement. The maximum cumulative hydrogen production of 743.5±14.4ml and 675.6±12.1ml and volumetric hydrogen production of 2.12L H2 /L substrate and 1.93L H2 /L substrate was recorded at 3.5% surfactin and 3.0% saponin respectively. Corresponding highest hydrogen yields were 79.2mlH 2 /gCarbo initial and 72.0mlH 2 /gCarbo initial respectively. Lag phase decreased from 12.5±2.0h at control to a minimum of 9.0±2.8h and 9.5±2.1h at 3.5% surfactin and 3.0% saponin respectively. Volatile fatty acid generation was increased with biosurfactants addition. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Stimulation of diesel degradation and biosurfactant production by aminoglycosides in a novel oil-degrading bacterium Pseudomonas luteola PRO23

    Directory of Open Access Journals (Sweden)

    Atanasković Iva M.

    2016-01-01

    Full Text Available Bioremediation is promising technology for dealing with oil hydrocarbons contamination. In this research growth kinetics and oil biodegradation efficiency of Pseudomonas luteola PRO23, isolated from crude oil-contaminated soil samples, were investigated under different concentrations (5, 10 and 20 g/L of light and heavy crude oil. More efficient biodegradation and more rapid adaptation and cell growth were obtained in conditions with light oil. The 5 to 10 g/L upgrade of light oil concentration stimulated the microbial growth and the biodegradation efficiency. Further upgrade of light oil concentration and the upgrade of heavy oil concentration both inhibited the microbial growth, as well as biodegradation process. Aminoglycosides stimulated biosurfactant production in P. luteola in the range of sub-inhibitory concentrations (0.3125, 0.625 μg/mL. Aminoglycosides also induced biofilm formation. The production of biosurfactants was the most intense during lag phase and continues until stationary phase. Aminoglycosides also induced changes in P. luteola growth kinetics. In the presence of aminoglycosides this strain degraded 82% of diesel for 96 h. These results indicated that Pseudomonas luteola PRO23 potentially can be used in bioremediation of crude oil-contaminated environments and that aminoglycosides could stimulate this process. [Projekat Ministarstva nauke Republike Srbije, br. TR31080

  6. Production and characterization of microbial biosurfactants for potential use in oil-spill remediation.

    Science.gov (United States)

    Marti, M E; Colonna, W J; Patra, P; Zhang, H; Green, C; Reznik, G; Pynn, M; Jarrell, K; Nyman, J A; Somasundaran, P; Glatz, C E; Lamsal, B P

    2014-02-05

    Two biosurfactants, surfactin and fatty acyl-glutamate, were produced from genetically-modified strains of Bacillus subtilis on 2% glucose and mineral salts media in shake-flasks and bioreactors. Biosurfactant synthesis ceased when the main carbohydrate source was completely depleted. Surfactin titers were ∼30-fold higher than fatty acyl-glutamate in the same medium. When bacteria were grown in large aerated bioreactors, biosurfactants mostly partitioned to the foam fraction, which was recovered. Dispersion effectiveness of surfactin and fatty acyl-glutamate was evaluated by measuring the critical micelle concentration (CMC) and dispersant-to-oil ratio (DOR). The CMC values for surfactin and fatty acyl-glutamate in double deionized distilled water were 0.015 and 0.10 g/L, respectively. However, CMC values were higher, 0.02 and 0.4 g/L for surfactin and fatty acyl-glutamate, respectively, in 12 parts per thousand Instant Ocean®[corrected].sea salt, which has been partly attributed to saline-induced conformational changes in the solvated ionic species of the biosurfactants. The DORs for surfactin and fatty acyl-glutamate were 1:96 and 1:12, respectively, in water. In Instant Ocean® solutions containing 12 ppt sea salt, these decreased to 1:30 and 1:4, respectively, suggesting reduction in oil dispersing efficiency of both surfactants in saline. Surfactant toxicities were assessed using the Gulf killifish, Fundulus grandis, which is common in estuarine habitats of the Gulf of Mexico. Surfactin was 10-fold more toxic than fatty acyl-glutamate. A commercial surfactant, sodium laurel sulfate, had intermediate toxicity. Raising the salinity from 5 to 25 ppt increased the toxicity of all three surfactants; however, the increase was the lowest for fatty acyl-glutamate. Copyright © 2013 Elsevier Inc. All rights reserved.

  7. Optimizing the use of biosurfactants to remove diesel contamination in porous media

    International Nuclear Information System (INIS)

    Cote, A.L.; Tumeo, M.A.

    1995-01-01

    It has been demonstrated that biosurfactants can be used to successfully enhance the removal of hydrocarbon contamination from soils. The Environmental Technology Laboratory (ETL) at the University of Alaska Fairbanks is currently involved in a multiyear study of surfactant usage in oil spill remediation in cold climates. Funding for this work is provided by the National Science Foundation, Petroleum Environmental Services, Inc., the Alaska Department of Conservation, and the University of Alaska Fairbanks. In the Summer of 1993, researchers from ETL successfully used a biologically derived surfactant to remove weathered crude oil contamination remaining from the Exxon Valdez oil spill. This same technology may be applicable to other hydrocarbon-contaminated sites. Subsequent laboratory studies are being performed using soil columns to quantify the interaction between surfactant usage and soil characteristics. Specifically, the amount of surfactant applied, the method of application, the level of diesel contamination, and the type of soil matrix are being investigated. Diesel fuel has been chosen as a common type of hydrocarbon contamination. Adsorption of the surfactant on particle surfaces within the soil matrix can increase the cost of surfactant application and potentially diminish oil recovery. Four soil types are being used in these studies; a well-sorted, medium-grained sand; a moderately-sorted gravel, a volcanically-derived soil and a silt representative of tundra conditions. All of these soils are frequently encountered in oil spill remediation. This paper focuses on the relationships being identified between the level of contamination, soil matrix type, and the effectiveness of contaminant removal by biologically-derived surfactants

  8. Production and characterisation of glycolipid biosurfactant by Halomonas sp. MB-30 for potential application in enhanced oil recovery.

    Science.gov (United States)

    Dhasayan, Asha; Kiran, G Seghal; Selvin, Joseph

    2014-12-01

    Biosurfactant-producing Halomonas sp. MB-30 was isolated from a marine sponge Callyspongia diffusa, and its potency in crude oil recovery from sand pack column was investigated. The biosurfactant produced by the strain MB-30 reduced the surface tension to 30 mN m(-1) in both glucose and hydrocarbon-supplemented minimal media. The critical micelle concentration of biosurfactant obtained from glucose-based medium was at 0.25 mg ml(-1) at critical micelle dilution 1:10. The chemical structure of glycolipid biosurfactant was characterised by infrared spectroscopy and proton magnetic resonance spectroscopy. The emulsification activity of MB-30 biosurfactant was tested with different hydrocarbons, and 93.1 % emulsification activity was exhibited with crude oil followed by kerosene (86.6 %). The formed emulsion was stable for up to 1 month. To identify the effectiveness of biosurfactant for enhanced oil recovery in extreme environments, the interactive effect of pH, temperature and salinity on emulsion stability with crude oil and kerosene was evaluated. The stable emulsion was formed at and above pH 7, temperature >80 °C and NaCl concentration up to 10 % in response surface central composite orthogonal design model. The partially purified biosurfactant recovered 62 % of residual crude oil from sand pack column. Thus, the stable emulsifying biosurfactant produced by Halomonas sp. MB-30 could be used for in situ biosurfactant-mediated enhanced oil recovery process and hydrocarbon bioremediation in extreme environments.

  9. Optimization of rhamnolipid production from Pseudomonas aeruginosa PBS towards application for microbial enhanced oil recovery.

    Science.gov (United States)

    Sharma, Rajni; Singh, Jagdish; Verma, Neelam

    2018-01-01

    The present work reveals the potential of biosurfactant producing P. aeruginosa PBS for microbial enhanced oil recovery (MEOR). The biosurfactant production medium and culture conditions were optimized using response surface methodology. The optimization of media components and process parameters was consecutively executed in two sets of experimental runs designed by central composite rotatable design (CCRD). The maximum biosurfactant yield was attained with 2% fresh inoculum of P. aeruginosa PBS in minimal salt medium (pH 7), possessing 2.17% sodium citrate as C-source and 0.5% yeast extract as N-source, after 48 h upon incubation at 30 °C/150 rpm. Under optimum conditions, biosurfactant yield was increased more than threefold and turned out to be 2.65 g/L as compared to 0.82 g/L under previous conditions. The biosurfactant was characterized as a glycolipid comprising of four rhamnolipid homologs (RhaRhaC 10 C 10 , RhaRhaC 8 C 10 , RhaRhaC 12 C 10 /RhaRhaC 10 C 12 , RhaC 10 C 10 ) by thin layer chromatography, fourier transform infrared spectroscopy, nuclear magnetic resonance and mass spectrometry. The produced biosurfactant was highly efficient for oil recovery application showing extreme reduction in surface tension of medium (71.80 to 23.76 mN/m), immense hydrocarbons emulsification capacity (50-60%) and greater stability at wide range of temperature (4-100 °C) and pH (4-10) along with an excellent (56.18 ± 1.59%) additional oil recovery in sand-pack column lab test.

  10. Coculture induced improved production of biosurfactant by Staphylococcus lentus SZ2: Role in protecting Artemia salina against Vibrio harveyi.

    Science.gov (United States)

    Hamza, Faseela; Kumar, Ameeta Ravi; Zinjarde, Smita

    2018-07-01

    Coculturing microorganisms can lead to enhanced production of bioactive compounds as a result of cross-species or cross-genera interactions. In this study, we demonstrate improved production of the biosurfactant (BS-SLSZ2 with antibiofilm properties) by the marine epibiotic bacterium Staphylococcus lentus SZ2 after cross-genera interactions with an aquaculture pathogen Vibrio harveyi. In cocultures, growth of V. harveyi was completely inhibited and resultant biofilms were exclusively composed of S. lentus. The cell free supernatant (CFS) derived from cocultures displayed improved antibiofilm activity with enhanced contents of BS-SLSZ2 compared to monocultured S. lentus. During coculture experiments, after short periods of incubation (6 and 12 h), 2.3 fold increased production of BS-SLSZ2 was observed. Planktonic growth of V. harveyi was also inhibited after coculturing with S. lentus as evidenced from plate culture-based studies and microscopic observations. The CFS derived from monocultures and cocultures did not display bactericidal activity and the observed inhibition of V. harveyi could be of competitive nature. During in vivo challenge experiments, S. lentus protected the model aquaculture system Artemia salina from V. harveyi infections. Seven days post infection, survival of the group of larvae infected with V. harveyi was 5 ± 4.47%. Better survival rates (73.33 ± 5.16%, comparable with the unexposed group) were observed in the group of larvae incubated with S. lentus and V. harveyi. This study highlights increased biosurfactant production by cocultured S. lentus and the application of this bacterium as a protective probiotic strain for inclusion in aquaculture practices. Copyright © 2018 Elsevier Inc. All rights reserved.

  11. Biosurfactants from marine microorganisms

    Directory of Open Access Journals (Sweden)

    Suppasil Maneerat

    2005-11-01

    Full Text Available Biosurfactants are the surface-active molecules synthesized by microorganisms. With the advantage of environmental compatibility, the demand for biosurfactants has been steadily increasing and may eventually replace their chemically synthesized counterparts. Marine biosurfactants produced by some marine microorganisms have been paid more attention, particularly for the bioremediation of the sea polluted by crude oil. This review describes screening of biosurfactant-producing microorganisms, the determination of biosurfactant activity as well as the recovery of marine surfactant. The uses of marine biosurfactants for bioremediation are also discussed.

  12. Biosurfactants and their role in oil recovery

    Energy Technology Data Exchange (ETDEWEB)

    McInerney, Michael J. [University of Oklahoma (United States)

    2011-07-01

    This paper presents the role of biosurfactants in oil recovery. Types of biosurfactants include, among others, lipopeptides, rhamnolipids, sophorolipids. The process of oil recovery and the involvement of microbes are explained. The objective is to know if lipopeptide biosurfactants lower interfacial tension. Fatty acid composition is important for lipopeptide biosurfactant activity and microbial surfactants are hydrophilic and Interfacial Tension (IFT) values are high. Examples of biosurfactants with lower IFT values with mixtures are also given. An experiment was conducted to determine whether lipopeptides recovery entrapped oil or not. The procedure and experimental setup are shown. It is seen that with higher concentration of biosurfactants, the percentage of residual oil recovery is higher. Another experiment was conducted to see if biosurfactants greater than 40 mg/l can be produced in oil reservoirs. The experimental design and the analysis with the results are given. It was seen that more oil was produced. Conclusions from the study were, among other findings, that, in situ biosurfactant production and inoculation are possible.

  13. Effect of medium components on the production of a biosurfactant from Candida tropicalis applied to the removal of hydrophobic contaminants in soil.

    Science.gov (United States)

    Batista, Ranielly M; Rufino, Raquel D; Luna, Juliana M; de Souza, José Edson G; Sarubbo, Leonie A

    2010-05-01

    The influence of medium constituents on the production of biosurfactants by Candida tropicalis cultivated in waste frying oil was investigated according to a fractional factorial 2(5-1) design. The combined effect of the C/N(inorganic), C/Fe, C/Mg, and C/P ratios and yeast extract on surface tension reduction, biosurfactant yield, emulsification activity, and biomass were studied. The highest biosurfactant yield was reached when low C/Mg and low C/P ratio variables were combined, while the cell growth was favored by increasing the nitrogen concentration. The highest surface tension net decrease, on the other hand, was observed at low yeast extract concentration, low C/Fe, and high C/P ratios. Emulsification indices against lubrication and automobile waste oil of approximately 65 to 95% were observed. The crude biosurfactant produced in the medium--formulated with 2% waste frying oil, 0.067% NH4Cl, 0.025% MgSO4.7H2O, 0.067% KH2PO4, and 0.0026% FeCl3.6H2O--removed approximately 78 to 97% of the petroleum and motor oil adsorbed in sand samples.

  14. Effect of nutrients and fermentation conditions on the production of biosurfactants using rhizobacteria isolated from fique plants

    Directory of Open Access Journals (Sweden)

    Aura M. Pedroza-Rodríguez

    2010-12-01

    Full Text Available To isolate biosurfactant-producing microorganisms from the rhizosphere of fique and to select the best genus to evaluate theeffect of nutritional and fermentation conditions on the production of rhamnolipids. Materials and methods. Rhizospheric soil wassampled in three areas of Cauca. The best genus was selected for the experimental designs (Plackett Burman and 22 factorial and to find theproduction conditions for the growth kinetics at an Erlenmeyer flask scale. Results. Isolates from the rhizosphere of fique plants were fromgroups (or genera of Bacillus, Pseudomonas and Actinomycetes, being Pseudomonas the more responsive in preliminary testing foremulsification. From the results of the experimental designs and the kinetics of production, we found that rhamnose synthesis associatedwith rhamnolipids (3.2 g/l and emulsification (68% EC24 was significantly favored (p <0.0001 by cultivating an inoculum of 10% v/vof Pseudomonas fluorescens in a medium composed of: soybean oil 2% (v/v, K2HPO40.2% (w/v, yeast extract 0.4 g/l, NH4NO33.7 g/l, 1 ml trace elements (CoCl320 mg/l, H3BO330 mg/l, ZnSO410 mg/l, Cu2SO41 mg/l, Na2MoO43 mg/l, FeSO410 mg/l MnSO42,6 mg/l and pH 7.2. Conclusion. Of all the microbial genera isolated from the rhizosphere of fique, Pseudomonas fluorescens had the greatestpotential in the production of biosurfactants of the rhamnolipids family.

  15. Potential biosurfactant producing endophytic and epiphytic fungi ...

    African Journals Online (AJOL)

    João Marcelo Lima

    2016-06-15

    Jun 15, 2016 ... L., macrophytes collected from oil-contaminated waters, were studied to assess their potential for ... personal hygiene products and food processing, among ... Biosurfactant production was undertaken in 50 mL of culture.

  16. Antimicrobial biosurfactants from marine Bacillus circulans: extracellular synthesis and purification.

    Science.gov (United States)

    Mukherjee, S; Das, P; Sivapathasekaran, C; Sen, R

    2009-03-01

    To purify the biosurfactant produced by a marine Bacillus circulans strain and evaluate the improvement in surface and antimicrobial activities. The study of biosurfactant production by B. circulans was carried out in glucose mineral salts (GMS) medium using high performance thin layer chromatography (HPTLC) for quantitative estimation. The biosurfactant production by this strain was found to be growth-associated showing maximum biosurfactant accumulation at 26 h of fermentation. The crude biosurfactants were purified using gel filtration chromatography with Sephadex G-50 matrix. The purification attained by employing this technique was evident from UV-visible spectroscopy and TLC analysis of crude and purified biosurfactants. The purified biosurfactants showed an increase in surface activity and a decrease in critical micelle concentration values. The antimicrobial action of the biosurfactants was also enhanced after purification. The marine B. circulans used in this study produced biosurfactants in a growth-associated manner. High degree of purification could be obtained by using gel filtration chromatography. The purified biosurfactants showed enhanced surface and antimicrobial activities. The antimicrobial biosurfactant produced by B. circulans could be effectively purified using gel filtration and can serve as new potential drugs in antimicrobial chemotherapy.

  17. Alternative methodology for isolation of biosurfactant-producing bacteria

    OpenAIRE

    Krepsky, N.; Da Silva, FS.; Fontana, LF.; Crapez, MAC.

    2007-01-01

    Wide biosurfactant application on biorremediation is limited by its high production cost. The search for cheaper biossurfactant production alternatives has guided our study. The use of selective media containing sucrose (10 g.L-1) and Arabian Light oil (2 g.L-1) as carbon sources showed to be effective to screen and maintain biosurfactant-producing consortia isolated from mangrove hydrocarbon-contaminated sediment. The biosurfactant production was assayed by kerosene, gasoline and Arabian Lig...

  18. In-situ production of biosurfactants: An alternative method for dispersing and bioremediating marine oil spills

    International Nuclear Information System (INIS)

    Josefsen, K.; Sveum, P.; Ramstad, S.; Markussen, S.; Folkvord, K.; Krigsvoll, S.; Aune, R.; Storroe, I.

    1995-01-01

    A study of surfactant producing bacteria for the dispersion of oil spills was conducted. Isolation procedures, shoreline experiments, flume basin experiments, and simulated open sea meso-scale experiments and results were described. Bacteria strains were obtained from several locations world-wide, though more success was experienced with strains from colder regions. Two strains were used in the meso-scale experiment. A rapid reduction in the aliphatic fraction of the dispersed oil was observed. Most strains were found to be capable of dispersing crude oils with differing compositions. Efforts to emulsify oil-in-water on an artificial shoreline with biosurfactants producing bacteria showed only limited success in mobilizing the oil. 8 figs., 2 tabs., 14 refs

  19. Kinetics of hydrocarbon extraction from oil shale using biosurfactant producing bacteria

    Energy Technology Data Exchange (ETDEWEB)

    Haddadin, Malik S.Y.; Abou Arqoub, Ansam A.; Abu Reesh, Ibrahim [Faculty of Graduate Studies, Jordan University, Queen Rania Street, Amman, 11942 (Jordan); Haddadin, Jamal [Faculty of Agriculture, Mutah University, P.O. Box 59, Mutah 61710 (Jordan)

    2009-04-15

    This study was done to extract hydrocarbon compounds from El-Lajjun oil shale using biosurfactant produced from two strains Rhodococcus erythropolis and Rhodococcus ruber. The results have shown that, optimal biosurfactant production was found using naphthalene and diesel as a carbon source for R. erthropolis and R. ruber, respectively. Optimum nitrogen concentration was 9 g/l and 7 g/l for R. erthropolis and R. ruber, respectively. Optimum K{sub 2}HPO{sub 4} to KH{sub 2}PO{sub 4} ratio, temperature, pH, and agitation speeds were 2:1, 37 C, 7 and 200 rpm. Under optimal conditions R. erthropolis and R. ruber produced 5.67 and 6.9 g/l biosurfactant, respectively. Maximum recovery of oil achieved with hydrogen peroxide pre-treatment was 25% and 26% at biosurfactant concentration of 8 g/l and 4 g/l for R. erthropolis and R. ruber, respectively. The extent desorption of hydrocarbons from the pre-treated oil shale by biosurfactant were inversely related to the concentration of high molecular weight hydrocarbons, asphaltenes compounds. Pre-treatment of oil shale with hydrogen peroxide produced better improvement in aromatic compounds extraction in comparison with improvement which resulted from demineralization of the oil shale. (author)

  20. Kinetics of hydrocarbon extraction from oil shale using biosurfactant producing bacteria

    International Nuclear Information System (INIS)

    Haddadin, Malik S.Y.; Abou Arqoub, Ansam A.; Abu Reesh, Ibrahim; Haddadin, Jamal

    2009-01-01

    This study was done to extract hydrocarbon compounds from El-Lajjun oil shale using biosurfactant produced from two strains Rhodococcus erythropolis and Rhodococcus ruber. The results have shown that, optimal biosurfactant production was found using naphthalene and diesel as a carbon source for R. erthropolis and R. ruber, respectively. Optimum nitrogen concentration was 9 g/l and 7 g/l for R. erthropolis and R. ruber, respectively. Optimum K 2 HPO 4 to KH 2 PO 4 ratio, temperature, pH, and agitation speeds were 2:1, 37 deg. C, 7 and 200 rpm. Under optimal conditions R. erthropolis and R. ruber produced 5.67 and 6.9 g/l biosurfactant, respectively. Maximum recovery of oil achieved with hydrogen peroxide pre-treatment was 25% and 26% at biosurfactant concentration of 8 g/l and 4 g/l for R. erthropolis and R. ruber, respectively. The extent desorption of hydrocarbons from the pre-treated oil shale by biosurfactant were inversely related to the concentration of high molecular weight hydrocarbons, asphaltenes compounds. Pre- treatment of oil shale with hydrogen peroxide produced better improvement in aromatic compounds extraction in comparison with improvement which resulted from demineralization of the oil shale

  1. Biosurfactant and Degradative Enzymes Mediated Crude Oil Degradation by Bacterium Bacillus subtilis A1

    Science.gov (United States)

    Parthipan, Punniyakotti; Preetham, Elumalai; Machuca, Laura L.; Rahman, Pattanathu K. S. M.; Murugan, Kadarkarai; Rajasekar, Aruliah

    2017-01-01

    In this work, the biodegradation of the crude oil by the potential biosurfactant producing Bacillus subtilis A1 was investigated. The isolate had the ability to synthesize degradative enzymes such as alkane hydroxylase and alcohol dehydrogenase at the time of biodegradation of hydrocarbon. The biosurfactant producing conditions were optimized as pH 7.0, temperature 40°C, 2% sucrose and 3% of yeast extract as best carbon and nitrogen sources for maximum production of biosurfactant (4.85 g l-1). Specifically, the low molecular weight compounds, i.e., C10–C14 were completely degraded, while C15–C19 were degraded up to 97% from the total hydrocarbon pools. Overall crude oil degradation efficiency of the strain A1 was about 87% within a short period of time (7 days). The accumulated biosurfactant from the biodegradation medium was characterized to be lipopeptide in nature. The strain A1 was found to be more robust than other reported biosurfactant producing bacteria in degradation efficiency of crude oil due to their enzyme production capability and therefore can be used to remove the hydrocarbon pollutants from contaminated environment. PMID:28232826

  2. Production of biosurfactant from Bacillus licheniformis for microbial enhanced oil recovery and inhibition the growth of sulfate reducing bacteria

    Directory of Open Access Journals (Sweden)

    H.S. El-Sheshtawy

    2015-06-01

    Full Text Available In this study, the bacterium Bacillus licheniformis has been isolated from oil reservoir; the ability of this bacterium to produce a biosurfactant was detected. Surface properties of the produced biosurfactant were confirmed by determining the emulsification power as well as surface and interfacial tension. The crude biosurfactant has been extracted from supernatant culture growth, and the yield of crude biosurfactant was about 1 g/l. Also, chemical structure of the produced biosurfactant was confirmed using FTIR analysis. Results revealed that, the emulsification power has been increased up to 96% and the surface tension decreased from 72 of distilled water to 36 mN/m after 72 h of incubation. The potential application of this bacterial species in microbial-enhanced oil recovery (MEOR was investigated. The percent of oil recovery was 16.6% upon application in a sand pack column designed to stimulate an oil recovery. It also showed antimicrobial activity against the growth of different strains of SRB (sulfate reducing bacteria. Results revealed that a complete inhibition of SRB growth using 1.0% crude biosurfactant is achieved after 3 h.

  3. Statistical optimization for lipase production from solid waste of vegetable oil industry.

    Science.gov (United States)

    Sahoo, Rajesh Kumar; Kumar, Mohit; Mohanty, Swati; Sawyer, Matthew; Rahman, Pattanathu K S M; Sukla, Lala Behari; Subudhi, Enketeswara

    2018-04-21

    The production of biofuel using thermostable bacterial lipase from hot spring bacteria out of low-cost agricultural residue olive oil cake is reported in the present paper. Using a lipase enzyme from Bacillus licheniformis, a 66.5% yield of methyl esters was obtained. Optimum parameters were determined, with maximum production of lipase at a pH of 8.2, temperature 50.8°C, moisture content of 55.7%, and biosurfactant content of 1.693 mg. The contour plots and 3D surface responses depict the significant interaction of pH and moisture content with biosurfactant during lipase production. Chromatographic analysis of the lipase transesterification product was methyl esters, from kitchen waste oil under optimized conditions, generated methyl palmitate, methyl stearate, methyl oleate, and methyl linoleate.

  4. Correlation between Biosurfactants and Antifungal Activity of a Biocontrol Bacterium, Bacillus amyloliquefaciens LM11

    Directory of Open Access Journals (Sweden)

    Beom Ryong Kang

    2017-06-01

    Full Text Available Bacillus amyloliquefaciens LM11 was isolated from the feces of larvae of the rhino beetle and showed strong antifungal activities against various phytopathogenic fungi by producing biosurfactants. In this study, our overall goal was to determine relationship between biosurfactants produced from the LM11 strain and its role in growth inhibition of phytopathogenic fungi. Production and expression levels of B. amyloliquefaciens LM11 biosurfactants were significantly differed depending on growth phases. Transcriptional and biochemical analysis indicated that the biosurfactants of the LM11 strain were greatly enhanced in late log-phase to stationary phase. Inhibitions of phytopathogenic mycelial growth and spore germination were directly correlated (P<0.001, R=0.761 with concentrations of the LM11 cell-free culture filtrates. The minimum inhibitory surface tension of the culture filtrate of the B. amyloliquefaciens LM11 grown in stationary phase to inhibit mycelial growth of the phytopathogenic fungi was 38.5 mN/m (P<0.001, R=0.951–0.977. Our results indicated that the biosurfactants of B. amyloliquefaciens LM11 act as key antifungal metabolites in biocontrol of plant diseases, and measuring surface tension of the cell-free culture fluids can be used as an easy indicator for optimal usage of the biocontrol agents.

  5. Isolation of biosurfactant producing bacteria from petroleum contaminated sites and their characterization

    Directory of Open Access Journals (Sweden)

    Rida Batool

    2017-05-01

    Full Text Available Biosurfactants are microbial amphiphilic compounds which can reduce surface tension between aqueous and hydrocarbon mixtures. Bacterial strains isolated from petroleum contaminated soil of various motor workshops were characterized morphologically and biochemically. Biosurfactant producing ability of the strains was determined and their emulsification activity was screened against different oils. All the selected bacterial strains showed enhanced biosurfactants production with yeast extract as nitrogen source and glucose as carbon source at optimized conditions. These strains also exhibited multiple metal and antibiotics resistance. Isolated biosurfactants of three most promising strains SF-1, SF-4 and SM-1 were extracted by solvent extraction and subjected to TLC technique. The technique indicates the glycolipid nature of the compounds and presence of rhamnose sugar, which was further confirmed by FT-IR analysis. 16srRNA analysis revealed that SF-1 and SM-1 had close resemblance with Pseudomonas sp. while SF-4 showed homology with Enterobacter sp. Isolation and screening of biosurfactant producing strains from petroleum polluted places proved to be a quick and effective means to find bacterial strains with possible industrial uses.

  6. Metagenomics for the discovery of novel biosurfactants of environmental interest from marine ecosystems.

    Science.gov (United States)

    Jackson, Stephen A; Borchert, Erik; O'Gara, Fergal; Dobson, Alan D W

    2015-06-01

    Research focused on the search for new biosurfactants aims to replace chemical surfactants, which while being cost-effective are ecologically undesirable. Metagenomics can lead to discovery of novel biosurfactants, tackling issues of low production yields. Recent successes include the heterologous production of biosurfactants. The dearth of biosurfactants discovered to date through metagenomics is puzzling given that good screening systems and heterologous host systems are available. Copyright © 2015 Elsevier Ltd. All rights reserved.

  7. Isolation of Biosurfactant Producing Bacteria from Oil Reservoirs

    OpenAIRE

    A Tabatabaee, M Mazaheri Assadi, AA Noohi,VA Sajadian

    2005-01-01

    Biosurfactants or surface-active compounds are produced by microoaganisms. These molecules reduce surface tension both aqueous solutions and hydrocarbon mixtures. In this study, isolation and identification of biosurfactant producing bacteria were assessed. The potential application of these bacteria in petroleum industry was investigated. Samples (crude oil) were collected from oil wells and 45 strains were isolated. To confirm the ability of isolates in biosurfactant production, haemolysis ...

  8. Influence of toluene and salinity on biosurfactant production by Bacillus sp.: scale up from flasks to a bench-scale bioreactor

    Directory of Open Access Journals (Sweden)

    Ellen Cristina Souza

    Full Text Available ABSTRACT To select the best biosurfactant producer, Pseudomonas putida, Bacillus megatherium, Bacillus licheniformis and Bacillus subtilis were cultured in flasks on media with different salinity [low salinity (LS, Bushnell-Haas (BH and artificial sea water (SW media] supplemented or not with toluene as a model pollutant. Toluene inhibited the growth of all microorganisms and stimulated the biosurfactant production. B. subtilis exhibited the best performance, being able to lower the surface tension (ST in the LS medium to 65.5 mN/min in the absence of toluene, and to 46.5 mN/min in the BH medium in the presence of toluene, corresponding to ST reductions of 13.0 and 27.5 mN/m, respectively. Scaling up the process to a bench-scale fermentor, the best results were obtained in the LS medium, where B. subtilis was able to reduce the toluene concentration from 26.0 to 4.3 g/L within 12 h and ST by 17.2 mN/m within 18 h. The results of this study point out that B. subtilis is an interesting biosurfactant producer, which could be used in the bioremediation of toluene-contaminated water.

  9. Biosurfactant-enhanced bioremediation of hydrophobic pollutants

    Energy Technology Data Exchange (ETDEWEB)

    Cameotra, S.S.; Makkar, R.S. [Inst. of Microbial Technology, Chandigarh (India)

    2010-01-15

    Biosurfactants are surface-active compounds synthesized by a wide variety of microorganisms. They are molecules that have both hydrophobic and - philic domains and are capable of lowering the surface tension and the interfacial tension of the growth medium. Biosurfactants possess different chemical structures-lipopeptides, glycolipids, neutral lipids, and fatty acids. They are nontoxic biomolecules that are biodegradable. Biosurfactants also exhibit strong emulsification of hydrophobic compounds and form stable emulsions. Polycyclic aromatic hydrocarbons (PAHs), crude on sludge, and pesticides call be toxic, mutagenic, and carcinogenic compounds that pollute the environment. They are released into the environment as a result of oil spillage and by-products of coal treatment processes. The low water solubility of these compounds limits their availability to microorganisms, which is a potential problem for bioremediation of contaminated sites. Microbially produced surfactants enhance the bioavailability of these hydrophobic compounds for bioremediation. Therefore, biosurfactant-enhanced solubility of pollutants has potential hioremediation applications.

  10. Formulation of a Commercial Biosurfactant for Application as a Dispersant of Petroleum and By-products Spilled in Oceans

    Directory of Open Access Journals (Sweden)

    Bruno Galdino Freitas

    2016-10-01

    Full Text Available Oil spills in oceans cause irreparable damage to marine life and harm the coastal populations of affected areas. It is therefore fundamental to develop treatment strategies for such spills. Currently, chemical dispersants have been used during oil spills, although these agents have been increasingly restricted due to their toxic potential. Thus, the aim of the present study was to formulate a biodegradable commercial biosurfactant for application as a dispersant. Biosurfactants are scientifically known biomolecules produced by microorganisms capable of allowing water-oil interaction. Thus, a biosurfactant was produced by the yeast Candida bombicola URM 3718 cultivated in industrial waste and formulated with the addition of a potassium sorbate preservative for fractionated sterilisation (tyndallisation and the combination of fluent vaporisation with the preservative. After formulation, samples were stored for 120 days, followed by surface tension, emulsification and oil dispersant tests in sea water. The results were promising for the biosurfactant formulated with the preservative, which demonstrated stability and an absence of toxicity in experiments with a marine indicator. The commercial biosurfactant was tested at different pH values, temperatures and in the presence of salt, demonstrating potential industrial application at a cost compatible with the environmental field. The formulation process developed in this research was patented in the Brazilian National Intellectual Property Institute (patent number BR1020140179631.

  11. Coal induced production of a rhamnolipid biosurfactant by Pseudomonas stutzeri, isolated from the formation water of Jharia coalbed.

    Science.gov (United States)

    Singh, Durgesh Narain; Tripathi, Anil Kumar

    2013-01-01

    A strain of Pseudomonas stutzeri was isolated form an enrichment of perchlorate reducing bacteria from the formation water collected from an Indian coalbed which solubilized coal and produced copious amount of biosurfactant when coal was added to the medium. It produced maximum biosurfactant with lignite coal followed by olive oil and soybean oil which was able to emulsify several aromatic hydrocarbons including kerosene oil, diesel oil, hexane, toluene etc. Haemolytic test, growth inhibition of Bacillus subtilis and FTIR analysis showed rhamnolipid nature of the biosurfactant. The stability of the coal induced biosurfactant in pH range of 4-8 and up to 25% NaCl concentration and 100 °C temperature suggests that due to its ability to produce biosurfactant and solubilize coal P. stutzeri may be useful in the coalbed for in situ biotransformation of coal into methane and in the bioremediation of PAHs from oil contaminated sites including marine environments. Copyright © 2012 Elsevier Ltd. All rights reserved.

  12. Bio-surfactants production from low cost substrate and degradation of diesel oil by a Rhodococcus strain; Production de biosurfactants sur un substrat economique et degradation du gasoil par une souche du genre Rhodococcus

    Energy Technology Data Exchange (ETDEWEB)

    Sadouk, Z.; Tazerouti, A. [Universite des Sciences et de la Technologie H. Boumediene (USTHB), Lab. de Synthese Organique, Faculte de Chimie, Algiers (Algeria); Sadouk, Z.; Hacene, H. [Universite des Sciences et de la Technologie H. Boumediene (USTHB), Lab. de Microbiologie, Faculte des Sciences Biologiques, Algiers (Algeria)

    2008-07-01

    The ability of a Rhodococcus strain to produce surface-active agents from residual sunflower frying oil (RSFO) has been screened in batch cultures. During cultivation with RSFO at the concentration 3% (vol/vol), the strain has synthesized extra-cellular compounds which increase the E{sub 24} emulsion index of the culture medium up to 63%. In their crude form, these substances lower the surface tension of water until 31.9 mN m{sup -1}. The exponential growth with RSFO as the sole carbon source has developed at a specific growth rate {mu} = 0.55 d{sup -1}. The critical micelle concentration of the crude product reached the value 287 mg L{sup -1} ({gamma}CMC = 31.9 mN m{sup -1}). After methyl-esterification, the lipid fraction of bio-surfactants has been analyzed by GC-MS in EI, which reveals the presence of fatty acid methyl esters. The microorganism was also cultivated with the diesel oil as the sole carbon source at the concentration 1% (vol/vol): the active growth phase has developed at rate = 0.02 d{sup -1}, without production of emulsifying substance: the microorganism seems to develop different modes of substrate uptake, according to the nature of the carbon source. The potential use of surface-active agents synthesized on RSFO by Rhodococcus erythropolis 16 LM.USTHB is in the oil industry with minimum purity specification, so that crude preparation could be used, at low cost, in clean-up of hydrocarbons contaminated sites and for enhanced oil recovery. (authors)

  13. Optimal Product Placement.

    Science.gov (United States)

    Hsu, Chia-Ling; Matta, Rafael; Popov, Sergey V; Sogo, Takeharu

    2017-01-01

    We model a market, such as an online software market, in which an intermediary connects sellers and buyers by displaying sellers' products. With two vertically-differentiated products, an intermediary can place either: (1) one product, not necessarily the better one, on the first page, and the other hidden on the second page; or (2) both products on the first page. We show that it can be optimal for the intermediary to obfuscate a product-possibly the better one-since this weakens price competition and allows the sellers to extract a greater surplus from buyers; however, it is not socially optimal. The choice of which one to obfuscate depends on the distribution of search costs.

  14. Bio-surfactants production from low cost substrate and degradation of diesel oil by a Rhodococcus strain

    International Nuclear Information System (INIS)

    Sadouk, Z.; Tazerouti, A.; Sadouk, Z.; Hacene, H.

    2008-01-01

    The ability of a Rhodococcus strain to produce surface-active agents from residual sunflower frying oil (RSFO) has been screened in batch cultures. During cultivation with RSFO at the concentration 3% (vol/vol), the strain has synthesized extra-cellular compounds which increase the E 24 emulsion index of the culture medium up to 63%. In their crude form, these substances lower the surface tension of water until 31.9 mN m -1 . The exponential growth with RSFO as the sole carbon source has developed at a specific growth rate μ = 0.55 d -1 . The critical micelle concentration of the crude product reached the value 287 mg L -1 (γCMC = 31.9 mN m -1 ). After methyl-esterification, the lipid fraction of bio-surfactants has been analyzed by GC-MS in EI, which reveals the presence of fatty acid methyl esters. The microorganism was also cultivated with the diesel oil as the sole carbon source at the concentration 1% (vol/vol): the active growth phase has developed at rate = 0.02 d -1 , without production of emulsifying substance: the microorganism seems to develop different modes of substrate uptake, according to the nature of the carbon source. The potential use of surface-active agents synthesized on RSFO by Rhodococcus erythropolis 16 LM.USTHB is in the oil industry with minimum purity specification, so that crude preparation could be used, at low cost, in clean-up of hydrocarbons contaminated sites and for enhanced oil recovery. (authors)

  15. Biosurfactant-enhanced soil bioremediation

    Energy Technology Data Exchange (ETDEWEB)

    Kosaric, N.; Lu, G.; Velikonja, J. [Univ. of Western Ontario, London, Ontario (Canada)

    1995-12-01

    Bioremediation of soil contaminated with organic chemicals is a viable alternative method for clean-up and remedy of hazardous waste sites. The final objective in this approach is to convert the parent toxicant into a readily biodegradable product which is harmless to human health and/or the environment. Biodegradation of hydrocarbons in soil can also efficiently be enhanced by addition or in-situ production of biosufactants. It was generally observed that the degradation time was shortened and particularly the adaptation time for the microbes. More data from our laboratories showed that chlorinated aromatic compounds, such as 2,4-dichlorophenol, a herbicide Metolachlor, as well as naphthalene are degraded faster and more completely when selected biosurfactants are added to the soil. More recent data demonstrated an enhanced biodegradation of heavy hydrocarbons in petrochemical sludges, and in contaminated oil when biosurfactants were present or were added prior to the biodegradation process.

  16. Optimizing production under uncertainty

    DEFF Research Database (Denmark)

    Rasmussen, Svend

    This Working Paper derives criteria for optimal production under uncertainty based on the state-contingent approach (Chambers and Quiggin, 2000), and discusses po-tential problems involved in applying the state-contingent approach in a normative context. The analytical approach uses the concept...... of state-contingent production functions and a definition of inputs including both sort of input, activity and alloca-tion technology. It also analyses production decisions where production is combined with trading in state-contingent claims such as insurance contracts. The final part discusses...

  17. Environmental applications for biosurfactants

    Energy Technology Data Exchange (ETDEWEB)

    Mulligan, Catherine N. [Department Building, Civil and Environmental Engineering, Concordia University, 1455 de Maisonneuve Boulevard W., Montreal, Quebec, H3G 1M8 (Canada)]. E-mail: mulligan@civil.concordia.ca

    2005-01-01

    Biosurfactants are surfactants that are produced extracellularly or as part of the cell membrane by bacteria, yeasts and fungi. Examples include Pseudomonas aeruginosa which produces rhamnolipids, Candida (formerly Torulopsis) bombicola, one of the few yeasts to produce biosurfactants, which produces high yields of sophorolipids from vegetable oils and sugars and Bacillus subtilis which produces a lipopeptide called surfactin. This review includes environmental applications of these biosurfactants for soil and water treatment. Biosurfactant applications in the environmental industries are promising due to their biodegradability, low toxicity and effectiveness in enhancing biodegradation and solubilization of low solubility compounds. However, more information is needed to be able to predict and model their behaviour. Full scale tests will be required. The role of biosurfactants in natural attenuation processes has not been determined. Very little information is available concerning the influence of soil components on the remediation process with biosurfactants. As most of the research until now has been performed with rhamnolipids, other biosurfactants need to be investigated as they may have more promising properties. - More information is needed to be able to predict and model the behaviour of biosurfactants.

  18. Environmental applications for biosurfactants

    International Nuclear Information System (INIS)

    Mulligan, Catherine N.

    2005-01-01

    Biosurfactants are surfactants that are produced extracellularly or as part of the cell membrane by bacteria, yeasts and fungi. Examples include Pseudomonas aeruginosa which produces rhamnolipids, Candida (formerly Torulopsis) bombicola, one of the few yeasts to produce biosurfactants, which produces high yields of sophorolipids from vegetable oils and sugars and Bacillus subtilis which produces a lipopeptide called surfactin. This review includes environmental applications of these biosurfactants for soil and water treatment. Biosurfactant applications in the environmental industries are promising due to their biodegradability, low toxicity and effectiveness in enhancing biodegradation and solubilization of low solubility compounds. However, more information is needed to be able to predict and model their behaviour. Full scale tests will be required. The role of biosurfactants in natural attenuation processes has not been determined. Very little information is available concerning the influence of soil components on the remediation process with biosurfactants. As most of the research until now has been performed with rhamnolipids, other biosurfactants need to be investigated as they may have more promising properties. - More information is needed to be able to predict and model the behaviour of biosurfactants

  19. Yeast glycolipid biosurfactants.

    Science.gov (United States)

    Jezierska, Sylwia; Claus, Silke; Van Bogaert, Inge

    2017-10-25

    Various yeasts, both conventional and exotic ones, are known to produce compounds useful to mankind. Ethanol is the most known of these compounds, but more complex molecules such as amphiphilic biosurfactants can also be derived from eukaryotic microorganisms at an industrially and commercially relevant scale. Among them, glycolipids are the most promising, due to their attractive properties and high product titers. Many of these compounds can be considered as secondary metabolites with a specific function for the host. Hence, a dedicated biosynthetic process enables regulation and combines pathways delivering the lipidic moiety and the hydrophilic carbohydrate part of the glycolipid. In this Review, we will discuss the biosynthetic and regulatory aspects of the yeast-derived sophorolipids, mannosylerythritol lipids, and cellobiose lipids, with special emphasis on the relation between glycolipid synthesis and the general lipid metabolism. © 2017 Federation of European Biochemical Societies.

  20. Assessment of four different methods for selecting biosurfactant ...

    African Journals Online (AJOL)

    ... and ease of use to screen biosurfactant producing six extremely halophilic bacteria isolated from saline soil of Chott El Hodna-M'sila (Algeria), which is considered as a thalassohaline environment. Results from screening methods revealed that, CH2 and CH5 strains are potential candidates for biosurfactant production.

  1. Two schemes for production of biosurfactant from Pseudomonas aeruginosa MR01: Applying residues from soybean oil industry and silica sol-gel immobilized cells.

    Science.gov (United States)

    Bagheri Lotfabad, Tayebe; Ebadipour, Negisa; Roostaazad, Reza; Partovi, Maryam; Bahmaei, Manochehr

    2017-04-01

    Rhamnolipids are the most common biosurfactants and P. aeruginosa strains are the most frequently studied microorganisms for the production of rhamnolipids. Eco-friendly advantages and promising applications of rhamnolipids in various industries are the major reasons for pursuing the economic production of these biosurfactants. This study shows that cultivation of P. aeruginosa MR01 in medium contained inexpensive soybean oil refinery wastes which exhibited similar levels and homologues of rhamnolipids. Mass spectrometry indicated that the Rha-C10-C10 and Rha-Rha-C10-C10 constitute the main rhamnolipids in different cultures of MR01 including one of oil carbon source analogues. Moreover, rhamnolipid mixtures extracted from different cultures showed critical micelle concentrations (CMC) in the range of ≃24 to ≃36mg/l with capability to reduce the surface tension of aqueous solution from 72 to ≃27-32mN/m. However, the sol-gel technique using tetraethyl orthosilicate (TEOS) was used as a gentler method in order to entrap the P. aeruginosa MR01 cells in mold silica gels. Immobilized cells can be utilized several times in consecutive fermentation batches as well as in flow fermentation processes. In this way, reusability of the cells may lead to a more economical fermentation process. Approximately 90% of cell viability was retained during the silica sol-gel immobilization and ≃84% of viability of immobilized cells was preserved for 365days of immobilization and storage of the cells in phosphate buffer at 4°C and 25°C. Moreover, mold gels showed good mechanical stability during the seven successive fermentation batches and the entrapped cells were able to efficiently preserve their biosurfactant-producing potential. Copyright © 2017 Elsevier B.V. All rights reserved.

  2. Real time production optimization

    Energy Technology Data Exchange (ETDEWEB)

    Saputelli, Luigi; Otavio, Joao; Araujo, Turiassu; Escorcia, Alvaro [Halliburton, Houston, TX (United States). Landmark Division

    2004-07-01

    Production optimization encompasses various activities of measuring, analyzing, modeling, prioritizing and implementing actions to enhance productivity of a field. We present a state-of-the-art framework for optimizing production on a continuous basis as new sensor data is acquired in real time. Permanently acquired data is modeled and analyzed in order to create predictive models. A model based control strategy is used to regulate well and field instrumentation. The optimum field operating point, which changes with time, satisfies the maximum economic return. This work is a starting point for further development in automatic, intelligent reservoir technologies which get the most out of the abilities of permanent, instrumented wells and remotely activated downhole completions. The strategy, tested with history-matched data from a compartmentalised giant field, proved to reduce operating costs while increasing oil recovery by 27% in this field. (author)

  3. DEVELOPMENT OF IMPROVED ANAEROBIC GROWTH OF BACILLUS MOJAVENSIS STRAIN JF-2 FOR THE PURPOSE OF IMPROVED ANAEROBIC BIOSURFACTANT PRODUCTION FOR ENHANCED OIL RECOVERY

    Energy Technology Data Exchange (ETDEWEB)

    M.J. McInerney; M. Folmsbee; D. Nagle

    2004-05-31

    for anaerobic growth and biosurfactant production in DNA-supplemented Medium E. In addition to DNA or deoxyribonucleosides, nitrate, amino acids and vitamins were all required for anaerobic growth of JF-2. Bacillus mojavensisT (ABO21191), Bacillus mojavensis, strain ROB2 also required DNA or deoxyribonucleosides for anaerobic growth. The improved anaerobic growth of Bacillus mojavensis JF-2 was a prerequisite for studies that will lead to improved anaerobic biosurfactant production.

  4. Introduction of Fusarium sp. UTMC 5039 as a potent fungal strain for biosurfactant production and evaluation of its potential for crude oil bioremediation

    Directory of Open Access Journals (Sweden)

    Hamid Moghimi

    2017-09-01

    Full Text Available Introduction: Biosurfactants are biological surface active agents which are used in many applications such as oil bioremediation of contaminated soils. Materials and methods: In this study, first soil samples were collected from crude oil contaminated regions of Iran. Fungal isolates were enriched in MSM medium supplemented with crude oil and purified and then all isolates were screened for biosurfactant activity. Then, the capacity of crude oil degradation in the selected isolate was measured using Total Petroleum Hydrocarbon (TPH assay by spectrophotometry and FT-IR analysis. Finally, morphological and molecular identification was carried out by sequencing amplification of beta-tubuline beta-tubulin and ITS gene. Results: Among 40 purified fungal isolated, the isolate SH-02 was selected as the best strain according to the oil spreading and parafilm M test., This isolate was purified from petroleum contaminated soil of Arak refinery. Morphological and molecular identification revealed that this isolate has 99% similarity to Fusarium redolens in ITS geneand was deposited in the University of Tehran Microorganisms Collection under the accession number, UTMC 5039. Measurement of surface tension reduction by Du Nouy Ring method showed that Fusarium sp. UTMC 5039 can reduce surface tension to 26.6 mN/m and this reduction amount is significant compared with the previous reports. According to the obtained results from TPH and FTIR assays,  60 % of crude oil was degraded biodegradation was measured for by  Fusarium sp. UTMC 5039. Discussion and conclusion: The current study results indicate that Fusarium sp. UTMC 5039 has a high capacity in biosurfactant production and introduced as a potent fungal strain for crude oil bioremediation.

  5. Biogeographical distribution analysis of hydrocarbon degrading and biosurfactant producing genes suggests that near-equatorial biomes have higher abundance of genes with potential for bioremediation.

    Science.gov (United States)

    Oliveira, Jorge S; Araújo, Wydemberg J; Figueiredo, Ricardo M; Silva-Portela, Rita C B; de Brito Guerra, Alaine; da Silva Araújo, Sinara Carla; Minnicelli, Carolina; Carlos, Aline Cardoso; de Vasconcelos, Ana Tereza Ribeiro; Freitas, Ana Teresa; Agnez-Lima, Lucymara F

    2017-07-27

    Bacterial and Archaeal communities have a complex, symbiotic role in crude oil bioremediation. Their biosurfactants and degradation enzymes have been in the spotlight, mainly due to the awareness of ecosystem pollution caused by crude oil accidents and their use. Initially, the scientific community studied the role of individual microbial species by characterizing and optimizing their biosurfactant and oil degradation genes, studying their individual distribution. However, with the advances in genomics, in particular with the use of New-Generation-Sequencing and Metagenomics, it is now possible to have a macro view of the complex pathways related to the symbiotic degradation of hydrocarbons and surfactant production. It is now possible, although more challenging, to obtain the DNA information of an entire microbial community before automatically characterizing it. By characterizing and understanding the interconnected role of microorganisms and the role of degradation and biosurfactant genes in an ecosystem, it becomes possible to develop new biotechnological approaches for bioremediation use. This paper analyzes 46 different metagenome samples, spanning 20 biomes from different geographies obtained from different research projects. A metagenomics bioinformatics pipeline, focused on the biodegradation and biosurfactant-production pathways, genes and organisms, was applied. Our main results show that: (1) surfactation and degradation are correlated events, and therefore should be studied together; (2) terrestrial biomes present more degradation genes, especially cyclic compounds, and less surfactation genes, when compared to water biomes; and (3) latitude has a significant influence on the diversity of genes involved in biodegradation and biosurfactant production. This suggests that microbiomes found near the equator are richer in genes that have a role in these processes and thus have a higher biotechnological potential. In this work we have focused on the

  6. Biosurfactants: promising bioactive molecules for oral-related health applications.

    Science.gov (United States)

    Elshikh, Mohamed; Marchant, Roger; Banat, Ibrahim M

    2016-09-01

    Biosurfactants are naturally produced molecules that demonstrate potentially useful properties such as the ability to reduce surface tensions between different phases. Besides having similar properties to their artificial chemical counterparts, they are regarded as environmental friendly, biodegradable and less toxic, which make them desirable candidates for downstream applications. The structure-activity-related properties of the biosurfactants which are directly correlated with potency of the biosurfactants as antimicrobial agents, the ability of the biosurfactants to alter surface energies and their ability to increase bioavailability are particularly what attract researchers to exploit their potential use in the oral-related health applications. Current research into biosurfactant indicates significant future potential for use in cosmetic and therapeutic oral hygiene product formulations and related medical device treatments. © FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  7. Production of sophorolipids biosurfactants by multiple species of the Starmerella (Candida) bombicola yeast clade

    Science.gov (United States)

    Sophorolipid production was tested for 26 strains representing 19 species of the Starmerella yeast clade, including S. bombicola and Candida apicola, which were previously reported to produce sophorolipids. Five of the 19 species tested showed significant production of sophorolipids: S. bombicola, ...

  8. Stimulation of rhamnolipid biosurfactants production in Pseudomonas aeruginosa AK6U by organosulfur compounds provided as sulfur sources.

    Science.gov (United States)

    Ismail, Wael; Shammary, Sultanah Al; El-Sayed, Wael S; Obuekwe, Christian; El Nayal, Ashraf M; Abdul Raheem, Abdul Salam; Al-Humam, Abdulmohsen

    2015-09-01

    A Pseudomonas aeruginosa AK6U strain produced rhamnolipid biosurfactants to variable extents when grown on MgSO 4 or organosulfur compounds as sulfur sources and glucose as a carbon source. Organosulfur cultures produced much higher biosurfactants amounts compared to the MgSO 4 cultures. The surface tension of the growth medium was reduced from 72 mN/m to 54 and 30 mN/m in cultures containing MgSO 4 and 4,6-dimethyldibenzothiophene (4,6-DM-DBT), respectively. AK6U cultures produced different rhamnolipid congener profiles depending on the provided sulfur source. The dibenzothiophene (DBT) culture produced more diverse and a higher number of rhamnolipid congeners as compared to the DBT-sulfone and MgSO 4 cultures. The number of mono-rhamnolipid congeners in the DBT culture was also higher than that detected in the DBT-sulfone and MgSO 4 cultures. Di-rhamnolipids dominated the congener profiles in all the analyzed cultures. The sulfur source can have a profound impact on the quality and quantity of the produced biosurfactants.

  9. Stimulation of rhamnolipid biosurfactants production in Pseudomonas aeruginosa AK6U by organosulfur compounds provided as sulfur sources

    Directory of Open Access Journals (Sweden)

    Wael Ismail

    2015-09-01

    Full Text Available A Pseudomonas aeruginosa AK6U strain produced rhamnolipid biosurfactants to variable extents when grown on MgSO4 or organosulfur compounds as sulfur sources and glucose as a carbon source. Organosulfur cultures produced much higher biosurfactants amounts compared to the MgSO4 cultures. The surface tension of the growth medium was reduced from 72 mN/m to 54 and 30 mN/m in cultures containing MgSO4 and 4,6-dimethyldibenzothiophene (4,6-DM-DBT, respectively. AK6U cultures produced different rhamnolipid congener profiles depending on the provided sulfur source. The dibenzothiophene (DBT culture produced more diverse and a higher number of rhamnolipid congeners as compared to the DBT-sulfone and MgSO4 cultures. The number of mono-rhamnolipid congeners in the DBT culture was also higher than that detected in the DBT-sulfone and MgSO4 cultures. Di-rhamnolipids dominated the congener profiles in all the analyzed cultures. The sulfur source can have a profound impact on the quality and quantity of the produced biosurfactants.

  10. Exponential fed-batch strategy for enhancing biosurfactant production by Bacillus subtilis.

    Science.gov (United States)

    Amin, G A

    2014-01-01

    Surfactin produced by Bacillus subtilis BDCC-TUSA-3 from Maldex-15 was used as a growth-associated product in a conventional batch process. Maldex-15 is a cheap industrial by-product recovered during manufacturing of high fructose syrup from corn starch. Surfactin production was greatly improved in exponential fed-batch fermentation. Maldex-15 and other nutrients were exponentially fed into the culture based on the specific growth rate of the bacterium. In order to maximize surfactin yield and productivity, conversion of different quantities of Maldex-15 into surfactin was investigated in five different fermentation runs. In all runs, most of the Maldex-15 was consumed and converted into surfactin and cell biomass with appreciable efficiencies. The best results were obtained with the fermentation run supplied with 204 g Maldex-15. Up to 36.1 g l(-1) of surfactin and cell biomass of 31.8 g l(-1) were achieved in 12 h. Also, a marked substrate yield of 0.272 g g(-1) and volumetric reactor productivity of 2.58 g 1(-1) h(-1) were obtained, confirming the establishment of a cost-effective commercial surfactin production.

  11. Alternative methodology for isolation of biosurfactant-producing bacteria

    Directory of Open Access Journals (Sweden)

    N. Krepsky

    Full Text Available Wide biosurfactant application on biorremediation is limited by its high production cost. The search for cheaper biossurfactant production alternatives has guided our study. The use of selective media containing sucrose (10 g.L-1 and Arabian Light oil (2 g.L-1 as carbon sources showed to be effective to screen and maintain biosurfactant-producing consortia isolated from mangrove hydrocarbon-contaminated sediment. The biosurfactant production was assayed by kerosene, gasoline and Arabian Light Emulsification activity and the bacterial growth curve was determined by bacterial quantification. The parameters analyzed for biosurfactant production were the growth curve, salinity concentration, flask shape and oxygenation. All bacteria consortia screened were able to emulsify the petroleum derivatives tested. Biosurfactant production increased according to the incubation time; however the type of emulsification (non-aqueous phase or aqueous phase did not change with time but with the compound tested. The methodology was able to isolate biosurfactant-producing consortia from superficial mangrove sediment contaminated by petroleum hydrocarbons and was recommended for selection of biosurfactant producing bacteria in tropical countries with low financial resources.

  12. Alternative methodology for isolation of biosurfactant-producing bacteria.

    Science.gov (United States)

    Krepsky, N; Da Silva, F S; Fontana, L F; Crapez, M A C

    2007-02-01

    Wide biosurfactant application on biorremediation is limited by its high production cost. The search for cheaper biossurfactant production alternatives has guided our study. The use of selective media containing sucrose (10 g x L(-1)) and Arabian Light oil (2 g x L(-1)) as carbon sources showed to be effective to screen and maintain biosurfactant-producing consortia isolated from mangrove hydrocarbon-contaminated sediment. The biosurfactant production was assayed by kerosene, gasoline and Arabian Light Emulsification activity and the bacterial growth curve was determined by bacterial quantification. The parameters analyzed for biosurfactant production were the growth curve, salinity concentration, flask shape and oxygenation. All bacteria consortia screened were able to emulsify the petroleum derivatives tested. Biosurfactant production increased according to the incubation time; however the type of emulsification (non-aqueous phase or aqueous phase) did not change with time but with the compound tested. The methodology was able to isolate biosurfactant-producing consortia from superficial mangrove sediment contaminated by petroleum hydrocarbons and was recommended for selection of biosurfactant producing bacteria in tropical countries with low financial resources.

  13. Pseudomonas Lipopeptide Biosurfactants

    DEFF Research Database (Denmark)

    Bonnichsen, Lise

    Pseudomonas lipopetide biosurfactants are amphiphilic molecules with a broad range of natural functions. Due to their surface active properties, it has been suggested that Pseudomonas lipopetides potentially play a role in biodegradation of hydrophobic compounds and have essential functions...... lipopetide biosurfactants in pollutant biodegradation and natural roles in biofilm formation. The work presented is a combination of environmental microbiology and exploiting genetic manipulation of pure cultures to achieve insightinto the effects and mechanisms of lipopeptides on microbial processes...

  14. Optimizing kaon production

    International Nuclear Information System (INIS)

    Berley, D.

    1976-01-01

    Kaon production data is examined with the view toward producing high quality beams in the future. Emphasis is placed upon the low momentum region, below 1 GeV/c, where the behavior of the kaon momentum spectrum is shown to be dominated by strongly momentum dependent kinematic factors. No complete experimental survey of production cross sections at different incident proton energies exists from a single apparatus. Information on relative production yields for different proton energies is important in the choice of parameters for an accelerator constructed primarily as a source of kaons. Data from different accelerators do exist and have been compiled. The key element in understanding how to present the data from different laboratories in a consistent way is an analysis of the effects of target material, target size, and production angle. This analysis not only enables a unification of the data but also indicates simple, useful and powerful principles about optimizing target efficiency. A summary is presented of existing kaon production facilities in the United States. These are all at proton accelerators. The possibility of using electron accelerators as kaon sources is examined. It is found that secondary beam fluxes can be obtained at electron linacs which are comparable to those obtained at proton synchrotrons

  15. Production of glycolipidic bio surfactants by environment bacteria: diversity and physiological part; Production de biosurfactants glycolipidiques par les bacteries de l`environnement: diversite et role physiologique

    Energy Technology Data Exchange (ETDEWEB)

    Arino, S

    1996-10-09

    About a hundred bacterial strains, isolated from soils, polluted or not by hydrocarbons, were tested for their capacity to excrete glycosides. The biggest productions were obtained for a soluble carbon source (glycerol) in a culture medium limited in the nitrogen source. In these conditions, 18 g/l of rhamnose lipids were produced by train Pseudomonas aeruginosa GL1 in a 200 h culture. Pseudomonas aeruginosa GL1, Cellulomonas celulans SA43 and Rhodococcus erythropolis DSM 43060 were studied in detail. The bio-surfactants produced were identified respectively as rhamnose lipids, oligosaccharide lipids and trehalose lipids, using various original analytical methods. Sugars and fatty acids composing these glycolipids had been shown to be usual components of the outer part of the cell wall in these microbial species. Moreover, cell hydrophobicity of the producing bacteria varied in time during culture. These results showed that both the cell wall and the extracellular glycolipids take part in the process of hydrocarbon uptake in the polluted environments. As other bacteria of the same species from different origins present the same characteristics, it may be concluded that glycolipid excretion does not constitute a specific response for hydrocarbon assimilation. In fact, a more general physiological role of glycolipids, concerning modifications of hydrophobic interfaces between the producing bacteria and their surrounding environment, could explain the production of glycolipids, and could also be utilized in hydrocarbon uptake. (author)

  16. Development of More Effective Biosurfactants for Enhanced Oil Recovery

    Energy Technology Data Exchange (ETDEWEB)

    McInerney, J.J.; Han, S.O.; Maudgalya, S.; Mouttaki, H.; Folmsbee, M.; Knapp, R.; Nagle, D.; Jackson, B.E.; Stuadt, M.; Frey, W.

    2003-01-16

    The objectives of this were two fold. First, core displacement studies were done to determine whether microbial processes could recover residual oil at elevated pressures. Second, the importance of biosurfactant production for the recovery of residual oil was studies. In these studies, a biosurfactant-producing, microorganisms called Bacillus licheniformis strain JF-2 was used. This bacterium produces a cyclic peptide biosurfactant that significantly reduces the interfacial tension between oil and brine (7). The use of a mutant deficient in surfactant production and a mathematical MEOR simulator were used to determine the major mechanisms of oil recovery by these two strains.

  17. Staphylococcus haemolyticus as a potential producer of biosurfactants with antimicrobial, anti-adhesive and synergistic properties.

    Science.gov (United States)

    Rossi, C C; Santos-Gandelman, J F; Barros, E M; Alvarez, V M; Laport, M S; Giambiagi-deMarval, M

    2016-09-01

    Staphylococcus haemolyticus is an opportunistic human pathogen that usually gains entry into the host tissue in association with medical device contamination. Biofilm formation is a key factor for the establishment of this bacterium and its arrangement and dynamics can be influenced by the synthesis of biosurfactants. Biosurfactants are structurally diverse amphiphilic molecules with versatile biotechnological applications, but information on their production by staphylococci is still scarce. In this work, two Staph. haemolyticus strains, showing high potential for biosurfactant production - as observed by four complementary methods - were investigated. Biosurfactant extracts were produced and studied for their capacity to inhibit the growth and biofilm formation by other bacterial human pathogens. The biosurfactant produced by the one of the strains inhibited the growth of most bacteria tested and subinhibitory concentrations of the biosurfactant were able to decrease biofilm formation and showed synergistic effects with tetracycline. Because these results were also positive when the biosurfactants were tested against the producing strains, it is likely that biosurfactant production by Staph. haemolyticus may be an unexplored virulence factor, important for competition and biofilm formation by the bacterium, in addition to the biotechnological potential. This work is the first to show the production of biosurfactants by Staphylococcus haemolyticus strains. Extracts showed antimicrobial, anti-adhesive and synergistic properties against a variety of relevant human pathogens, including the producing strains. In addition to the biotechnological potential, biosurfactants produced by Staph. haemolyticus are potentially undescribed virulence determinants in their producing strains. © 2016 The Society for Applied Microbiology.

  18. DEVELOPMENT OF BIOSURFACTANT-MEDIATED OIL RECOVERY IN MODEL POROUS SYSTEMS AND COMPUTER SIMULATIONS OF BIOSURFACTANT-MEDIATED OIL RECOVERY

    Energy Technology Data Exchange (ETDEWEB)

    M.J. McInerney; S.K. Maudgalya; R. Knapp; M. Folmsbee

    2004-05-31

    Current technology recovers only one-third to one-half of the oil that is originally present in an oil reservoir. Entrapment of petroleum hydrocarbons by capillary forces is a major factor that limits oil recovery (1, 3, 4). Hydrocarbon displacement can occur if interfacial tension (IFT) between the hydrocarbon and aqueous phases is reduced by several orders of magnitude. Microbially-produced biosurfactants may be an economical method to recover residual hydrocarbons since they are effective at low concentrations. Previously, we showed that substantial mobilization of residual hydrocarbon from a model porous system occurs at biosurfactant concentrations made naturally by B. mojavensis strain JF-1 if a polymer and 2,3-butanediol were present (2). In this report, we include data on oil recovery from Berea sandstone experiments along with our previous data from sand pack columns in order to relate biosurfactant concentration to the fraction of oil recovered. We also investigate the effect that the JF-2 biosurfactant has on interfacial tension (IFT). The presence of a co-surfactant, 2,3-butanediol, was shown to improve oil recoveries possibly by changing the optimal salinity concentration of the formulation. The JF-2 biosurfactant lowered IFT by nearly 2 orders of magnitude compared to typical values of 28-29 mN/m. Increasing the salinity increased the IFT with or without 2,3-butanediol present. The lowest interfacial tension observed was 0.1 mN/m. Tertiary oil recovery experiments showed that biosurfactant solutions with concentrations ranging from 10 to 60 mg/l in the presence of 0.1 mM 2,3-butanediol and 1 g/l of partially hydrolyzed polyacrylamide (PHPA) recovered 10-40% of the residual oil present in Berea sandstone cores. When PHPA was used alone, about 10% of the residual oil was recovered. Thus, about 10% of the residual oil recovered in these experiments was due to the increase in viscosity of the displacing fluid. Little or no oil was recovered at

  19. Emulsification of Hydrocarbons by Biosurfactant: Exclusive Use of Agrowaste

    Directory of Open Access Journals (Sweden)

    Olusola Solomon Amodu

    2014-04-01

    Full Text Available Novel biosurfactant-producing strains were isolated from hydrocarbon-contaminated environments that exclusively utilize agro-waste as their primary carbon source for the expression of biosurfactants. These were quantified using various standardized methods. Among the agro-waste screened, Beta vulgaris (Beetroot proved to be the most suitable substrate, for which the biosurfactants produced by three bacterial isolates–B. licheniformis STK01, B. subtilis STK02, and P. aeruginosa STK03–lowered the surface tension of the culture media to 30.0, 32.98, and 30.37 mN/m, respectively. The biosurfactants achieved considerable emulsification activity, particularly for heavy hydrocarbons, with the highest emulsification indices being 65.5% and 95% for anthracene and lubricant oil, respectively. The emulsion formed with lubricant oil was thermally stable even up to 50 °C for 21 days. The results showed the proficiency of the novel bacterial isolates used, as well as the suitability of solid agro-waste for biosurfactant production, thus suggesting that exclusive utilization of solid agro-waste is a promising option for use in biosurfactant production for environmental remediation. The outstanding emulsification activity and thermal stability demonstrated by the biosurfactants produced showed their potential applications in enhancing bioavailability and bioremediation of recalcitrant and hydrophobic environmental contaminants.

  20. Application of biosurfactant in oil spill management

    International Nuclear Information System (INIS)

    Juwarkar, A.; Babu, P.S.; Mishra, K.; Deshpande, M.

    1993-01-01

    Surfactants are surface active agents which reduce surface tension and interfacial tension between two immiscible phases and help in emulsification. Toxicity, nonbiodegradability, and limited structural types of chemical surfactants have initiated the need for effective substitutes. Biosurfactants, which are synthesized by specific microbial cultures, have surface active properties comparable to chemical surfactants. They are compounds that can help in oil spill cleanup operations without presenting the problem posed by chemical surfactants. Two bacterial cultures were isolated from oil-contaminated soil and were used for biosurfactant production. The biosurfactants produced by Bacillus licheniformis, BS1, and Pseudomonas aeruginosa, BS2, in mineral media containing glucose as the carbon source belong to the class of lipoprotein and glycolipid, respectively. They were found to reduce the surface and interfacial tension of water and water-hexadecane system from 72 dynes/cm and 40 dynes/cm to 28 to 30 dynes/cm and 1 to 3 dynes/cm, respectively. These results were comparable with chemical surfactants with respect to surface tension reduction (Slic Gone 34 dynes/ cm and Castrol 30 dynes/cm). The low interfacial tension allows the formation of stable emulsion. The two cultures were grown on different substrates, namely, glucose, mannitol, glycerol, hexadecane, oily sludge, and crude oil. Emulsion formation of hexadecane in water was tested with the cell-free broth containing biosurfactant from the respective substrate broths. Emulsions of 56% stability to 100% stability were obtained from these biosurfactant-containing broths. Both biosurfactants were able to emulsify crude oil. A surfactant's ability to form a stable emulsion is the first step in oil spill cleanup. The emulsified oil can then be acted upon very easily by the microorganism under study

  1. Evaluation of substrates from renewable-resources in biosurfactants ...

    African Journals Online (AJOL)

    Evaluation of substrates from renewable-resources in biosurfactants production by Pseudomonas strains. Sidnei Cerqueira dos Santos, Luzimar Gonzaga Fernandez, Juan Carlos Rossi-Alva, Milton Ricardo de Abreu Roque ...

  2. Trehalolipid biosurfactants from nonpathogenic Rhodococcus actinobacteria with diverse immunomodulatory activities.

    Science.gov (United States)

    Kuyukina, Maria S; Ivshina, Irena B; Baeva, Tatiana A; Kochina, Olesia A; Gein, Sergey V; Chereshnev, Valery A

    2015-12-25

    Actinobacteria of the genus Rhodococcus produce trehalolipid biosurfactants with versatile biochemical properties and low toxicity. In recent years, these biosurfactants are increasingly studied as possible biomedical agents with expressed immunological activities. Applications of trehalolipids from Rhodococcus, predominantly cell-bound, in biomedicine are also attractive because their cost drawback could be less significant for high-value products. The review summarizes recent findings in immunomodulatory activities of trehalolipid biosurfactants from nonpathogenic Rhodococcus and related actinobacteria and compares their biomedical potential with well-known immunomodifying properties of trehalose dimycolates from Mycobacterium tuberculosis. Molecular mechanisms of trehalolipid interactions with immunocompetent cells are also discussed. Copyright © 2015. Published by Elsevier B.V.

  3. In situ production of bio-surfactants: An alternative method for dispersing and bioremediating marine oil spills

    International Nuclear Information System (INIS)

    Josefsen, K.D.; Sveum, P.; Ramstad, P.; Markussen, S.; Folkvord, K.; Krigsvoll, K.; Aune, R.; Storroe, I.

    1995-01-01

    Some oil degrading bacteria are able to produce surfactants. These biosurfactants enhance dispersion of oil droplets into the water column. A large number of surfactant producing bacterial strains have been isolated from seawater samples collected at different sites around the world. Strains isolated from seawater samples collected in cold regions generally had better properties than strains isolated from warm seawater. Many of the isolated strains were able to disperse crude oils with a large variation of composition, as well as the water-in-emulsion (chocolate mousse) formed during weathering of crude oil in the sea. The results show that in situ application of surfactant producing bacteria can be a viable tool in future oil spill contingency, and that dispersion of oil may increase the biodegradation rate. Work is in progress to examine the use of such bacteria in the bioremediation of oil contaminated shorelines. 10 refs., 3 figs., 2 tabs

  4. Biosurfactants: Promising Molecules for Petroleum Biotechnology Advances

    Directory of Open Access Journals (Sweden)

    DARNE GERMANO DE ALMEIDA

    2016-10-01

    Full Text Available The growing global demand for sustainable technologies that improves the efficiency of petrochemical processes in the oil industry has driven advances in petroleum biotechnology in recent years. Petroleum industry uses substantial amounts of petrochemical-based synthetic surfactants in its activities as mobilizing agents to increase the availability or recovery of hydrocarbons as well as many other applications related to extraction, treatment, cleaning and transportation. However, biosurfactants have several potential applications for use across the oil processing chain and in the formulations of petrochemical products such as emulsifying/demulsifying agents, anticorrosive, biocides for sulphate-reducing bacteria, fuel formulation, extraction of bitumen from tar sands and many other innovative applications. Due to their versatility and proven efficiency, biosurfactants are often presented as valuable versatile tools that can transform and modernise petroleum biotechnology in an attempt to provide a true picture of state of the art and directions or use in the oil industry. We believe that biosurfactants are going to have a significant role in many future applications in the oil industries and in this review therefore, we highlight recent important relevant applications, patents disclosures and potential future applications for biosurfactants in petroleum and related industries.

  5. Biosurfactants: Promising Molecules for Petroleum Biotechnology Advances.

    Science.gov (United States)

    De Almeida, Darne G; Soares Da Silva, Rita de Cássia F; Luna, Juliana M; Rufino, Raquel D; Santos, Valdemir A; Banat, Ibrahim M; Sarubbo, Leonie A

    2016-01-01

    The growing global demand for sustainable technologies that improves the efficiency of petrochemical processes in the oil industry has driven advances in petroleum biotechnology in recent years. Petroleum industry uses substantial amounts of petrochemical-based synthetic surfactants in its activities as mobilizing agents to increase the availability or recovery of hydrocarbons as well as many other applications related to extraction, treatment, cleaning, and transportation. However, biosurfactants have several potential applications for use across the oil processing chain and in the formulations of petrochemical products such as emulsifying/demulsifying agents, anticorrosive, biocides for sulfate-reducing bacteria, fuel formulation, extraction of bitumen from tar sands, and many other innovative applications. Due to their versatility and proven efficiency, biosurfactants are often presented as valuable versatile tools that can transform and modernize petroleum biotechnology in an attempt to provide a true picture of state of the art and directions or use in the oil industry. We believe that biosurfactants are going to have a significant role in many future applications in the oil industries and in this review therefore, we highlight recent important relevant applications, patents disclosures and potential future applications for biosurfactants in petroleum and related industries.

  6. Biosurfactants: Promising Molecules for Petroleum Biotechnology Advances

    Science.gov (United States)

    De Almeida, Darne G.; Soares Da Silva, Rita de Cássia F.; Luna, Juliana M.; Rufino, Raquel D.; Santos, Valdemir A.; Banat, Ibrahim M.; Sarubbo, Leonie A.

    2016-01-01

    The growing global demand for sustainable technologies that improves the efficiency of petrochemical processes in the oil industry has driven advances in petroleum biotechnology in recent years. Petroleum industry uses substantial amounts of petrochemical-based synthetic surfactants in its activities as mobilizing agents to increase the availability or recovery of hydrocarbons as well as many other applications related to extraction, treatment, cleaning, and transportation. However, biosurfactants have several potential applications for use across the oil processing chain and in the formulations of petrochemical products such as emulsifying/demulsifying agents, anticorrosive, biocides for sulfate-reducing bacteria, fuel formulation, extraction of bitumen from tar sands, and many other innovative applications. Due to their versatility and proven efficiency, biosurfactants are often presented as valuable versatile tools that can transform and modernize petroleum biotechnology in an attempt to provide a true picture of state of the art and directions or use in the oil industry. We believe that biosurfactants are going to have a significant role in many future applications in the oil industries and in this review therefore, we highlight recent important relevant applications, patents disclosures and potential future applications for biosurfactants in petroleum and related industries. PMID:27843439

  7. Isolation of Biosurfactant Producing Bacteria from Oil Reservoirs

    Directory of Open Access Journals (Sweden)

    A Tabatabaee, M Mazaheri Assadi, AA Noohi,VA Sajadian

    2005-01-01

    Full Text Available Biosurfactants or surface-active compounds are produced by microoaganisms. These molecules reduce surface tension both aqueous solutions and hydrocarbon mixtures. In this study, isolation and identification of biosurfactant producing bacteria were assessed. The potential application of these bacteria in petroleum industry was investigated. Samples (crude oil were collected from oil wells and 45 strains were isolated. To confirm the ability of isolates in biosurfactant production, haemolysis test, emulsification test and measurement of surface tension were conducted. We also evaluated the effect of different pH, salinity concentrations, and temperatures on biosurfactant production. Among importance features of the isolated strains, one of the strains (NO.4: Bacillus.sp showed high salt tolerance and their successful production of biosurfactant in a vast pH and temperature domain and reduced surface tension to value below 40 mN/m. This strain is potential candidate for microbial enhanced oil recovery. The strain4 biosurfactant component was mainly glycolipid in nature.

  8. Evaluation and functional characterization of a biosurfactant produced by Lactobacillus plantarum CFR 2194.

    Science.gov (United States)

    Madhu, Arenahalli Ningegowda; Prapulla, Siddalingaiya Gurudutt

    2014-02-01

    The study details the investigations on the ability of Lactobacillus plantarum CFR 2194, an isolate from kanjika, a rice-based ayurvedic fermented product, to produce biosurfactant. Surfactant production, as a function of fermentation time, indicates that the maximum production occurred at 72 h under stationary conditions. Isolation, partial purification, and characterization of the biosurfactant produced have been carried out, and Fourier transform infrared spectroscopy (FTIR) spectra demonstrated that biosurfactants were constituted by protein and polysaccharide fractions, i.e., possessed the structure typical of glycoprotein, which is affected by the medium composition and the phase of growth of the biosurfactant-synthesizing strain. Critical micelle concentration (cmc) of the biosurfactant was found to be 6 g l(-1). The emulsification index (EI), emulsification activity (EA), and emulsion stability (ES) values of the biosurfactant have confirmed its emulsification property. Aqueous fractions of the produced biosurfactant exhibited a significant antimicrobial activity against the food-borne pathogenic species: Escherichia coli ATCC 31705, E. coli MTCC 108, Salmonella typhi, Yersinia enterocolitica MTCC 859, and Staphylococcus aureus F 722. More importantly, the biosurfactant from L. plantarum showed antiadhesive property against above food-borne pathogens. The results thus indicate the potential for developing strategies to prevent microbial colonization of food contact surfaces and health-care prosthesis using these biosurfactants.

  9. The emulsifying effect of biosurfactants produced by food spoilage organisms in Nigeria

    Directory of Open Access Journals (Sweden)

    Christianah O. Ogunmola

    2016-04-01

    Full Text Available Food spoilage organisms were isolated using standard procedures on Nutrient Agar, Cetrimide Agar and Pseudomonas Agar Base (supplemented with CFC. The samples were categorized as animal products (raw fish, egg, raw chicken, corned beef, pasteurized milk and plant products (vegetable salad, water leaf (Talinium triangulare, boiled rice, tomatoes and pumpkin leaf (Teifairia occidentalis.They were characterised as Pseudomonas putida, Pseudomonas aeruginosa, Pseudomonas stutzeri, Burkholderia pseudomallei, Serratia rubidaea, Corynebacterium pilosum, Bacillus subtilis, Bacillus mycoides, Bacillus laterosporus, Bacillus laterosporus, Serratia marcescens, Bacillus cereus, Bacillus macerans, Alcaligenes faecalis and Alcaligenes eutrophus. Preliminary screening for biosurfactant production was done using red blood haemolysis test and confirmed by slide test, drop collapse and oil spreading assay. The biosurfactant produced was purified using acetone and the composition determined initially using Molisch’s test, thin layer chromatography and gas chromatography mass spectrometry. The components were found to be ethanol, amino acids, butoxyacetic acid, hexadecanoic acid, oleic acid, lauryl peroxide, octadecanoic acid and phthalic acid. The producing organisms grew readily on several hydrocarbons such as crude oil, diesel oil and aviation fuel when used as sole carbon sources.  The purified biosurfactants produced were able to cause emulsification of kerosene (19.71-27.14% as well as vegetable oil (16.91-28.12% based on the emulsification index. This result suggests that the isolates can be an asset and further work can exploit their optimal potential in industries.

  10. Characterization and emulsification properties of rhamnolipid and sophorolipid biosurfactants and their applications.

    Science.gov (United States)

    Nguyen, Thu T; Sabatini, David A

    2011-02-18

    Due to their non-toxic nature, biodegradability and production from renewable resources, research has shown an increasing interest in the use of biosurfactants in a wide variety of applications. This paper reviews the characterization of rhamnolipid and sophorolipid biosurfactants based on their hydrophilicity/hydrophobicity and their ability to form microemulsions with a range of oils without additives. The use of the biosurfactants in applications such as detergency and vegetable oil extraction for biodiesel application is also discussed. Rhamnolipid was found to be a hydrophilic surfactant while sophorolipid was found to be very hydrophobic. Therefore, rhamnolipid and sophorolipid biosurfactants in mixtures showed robust performance in these applications.

  11. Characterization and Emulsification Properties of Rhamnolipid and Sophorolipid Biosurfactants and Their Applications

    Directory of Open Access Journals (Sweden)

    Thu T. Nguyen

    2011-02-01

    Full Text Available Due to their non-toxic nature, biodegradability and production from renewable resources, research has shown an increasing interest in the use of biosurfactants in a wide variety of applications. This paper reviews the characterization of rhamnolipid and sophorolipid biosurfactants based on their hydrophilicity/hydrophobicity and their ability to form microemulsions with a range of oils without additives. The use of the biosurfactants in applications such as detergency and vegetable oil extraction for biodiesel application is also discussed. Rhamnolipid was found to be a hydrophilic surfactant while sophorolipid was found to be very hydrophobic. Therefore, rhamnolipid and sophorolipid biosurfactants in mixtures showed robust performance in these applications.

  12. Optimal taxation with household production

    DEFF Research Database (Denmark)

    Kleven, Henrik Jacobsen; Richter, Wolfram F.; Sørensen, Peter Birch

    2000-01-01

    on consumer services even if such services are complements to leisure. Second, we find that when services and other goods are equally substitutable for leisure, so that uniform commodity taxation would be optimal in the absence of home production, the optimal tax structure will certainly involve a relatively......This paper suggests that the optimal tax system should favour market-produced services which are close substitutes for home-produced services. First, we modify the classical Corlett-Hague rule for optimal commodity taxation by showing that it may be optimal to impose a relatively low tax rate...... low tax rate on consumer services...

  13. Unexplored Brazilian oceanic island host high salt tolerant biosurfactant-producing bacterial strains.

    Science.gov (United States)

    da Silva, Fábio Sérgio Paulino; Pylro, Victor Satler; Fernandes, Pericles Leonardo; Barcelos, Gisele Souza; Kalks, Karlos Henrique Martins; Schaefer, Carlos Ernesto Gonçalves Reynaud; Tótola, Marcos Rogério

    2015-05-01

    We aimed to isolate biosurfactant-producing bacteria in high salt conditions from uncontaminated soils on the Brazilian oceanic island, Trindade. Blood agar medium was used for the isolation of presumptive biosurfactant-producing bacteria. Confirmation and measurements of biosurfactant production were made using an oil-spreading method. The isolates were identified by fatty acid profiles and partial 16S rRNA gene sequence analysis. A total of 14 isolates obtained from the 12 soil samples were found to produce biosurfactants. Among them, two isolates stood out as being able to produce biosurfactant that is increasingly active in solutions containing up to 175 g L(-1) NaCl. These high salt tolerant biosurfactant producers are affiliated to different species of the genus Bacillus. Soil organic matter showed positive correlation with the number of biosurfactant-producing bacteria isolated from our different sampling sites. The applied approach successfully recovered and identified biosurfactant-producing bacteria from non-contaminated soils. Due to the elevated salt tolerance, as well as their capacity to produce biosurfactants, these isolates are promising for environmental biotechnological applications, especially in the oil production chain.

  14. On optimization of power production

    Energy Technology Data Exchange (ETDEWEB)

    Feltenmark, S.

    1997-01-01

    Short-term optimization of power production is treated. It concerns the problem of determining a production schedule for a power system, which minimizes the total cost of production, while satisfying various constraints. The thesis consists of an introductory chapter, four chapters that each concerns a specific problem area (economic dispatch, unit commitment, hydro power planning and cogeneration optimization), plus a chapter with relevant theory. The emphasis of the thesis is on the mathematical structures that arise in problems in this field, and how to exploit them algorithmically. A recurring theme is convexification, either implicit, by dualization, or explicit, as in our approach to hydro power optimization. 134 refs

  15. Microbial biosurfactants as additives for food industries.

    Science.gov (United States)

    Campos, Jenyffer Medeiros; Stamford, Tânia Lúcia Montenegro; Sarubbo, Leonie Asfora; de Luna, Juliana Moura; Rufino, Raquel Diniz; Banat, Ibrahim M

    2013-01-01

    Microbial biosurfactants with high ability to reduce surface and interfacial surface tension and conferring important properties such as emulsification, detergency, solubilization, lubrication and phase dispersion have a wide range of potential applications in many industries. Significant interest in these compounds has been demonstrated by environmental, bioremediation, oil, petroleum, food, beverage, cosmetic and pharmaceutical industries attracted by their low toxicity, biodegradability and sustainable production technologies. Despite having significant potentials associated with emulsion formation, stabilization, antiadhesive and antimicrobial activities, significantly less output and applications have been reported in food industry. This has been exacerbated by uneconomical or uncompetitive costing issues for their production when compared to plant or chemical counterparts. In this review, biosurfactants properties, present uses and potential future applications as food additives acting as thickening, emulsifying, dispersing or stabilising agents in addition to the use of sustainable economic processes utilising agro-industrial wastes as alternative substrates for their production are discussed. © 2013 American Institute of Chemical Engineers.

  16. Biosurfactants in cosmetic formulations: trends and challenges.

    Science.gov (United States)

    Vecino, X; Cruz, J M; Moldes, A B; Rodrigues, L R

    2017-11-01

    Cosmetic products play an essential role in everyone's life. People everyday use a large variety of cosmetic products such as soap, shampoo, toothpaste, deodorant, skin care, perfume, make-up, among others. The cosmetic industry encompasses several environmental, social and economic impacts that are being addressed through the search for more efficient manufacturing techniques, the reduction of waste and emissions and the promotion of personal hygiene, contributing to an improvement of public health and at the same time providing employment opportunities. The current trend among consumers is the pursuit for natural ingredients in cosmetic products, as many of these products exhibit equal, better or additional benefits in comparison with the chemical-based products. In this sense, biosurfactants are natural compounds with great potential in the formulation of cosmetic products given by their biodegradability and impact in health. Indeed, many of these biosurfactants could exhibit a "prebiotic" character. This review covers the current state-of-the-art of biosurfactant research for cosmetic purposes and further discusses the future challenges for cosmetic applications.

  17. Screening concepts for the isolation of biosurfactant producing microorganisms.

    Science.gov (United States)

    Walter, Vanessa; Syldatk, Christoph; Hausmann, Rudolf

    2010-01-01

    This chapter gives an overview of current methods for the isolation of biosurfactant producing microbes. The common screening methods for biosurfactants are presented. Sampling and isolation of bacteria are the basis for screening of biosurfactant producing microbes. Hydrocarbon-contaminated sites are the most promising for the isolation of biosurfactant producing microbes, but many strains have also been isolated from undisturbed sites. In subsequent steps the isolates have to be characterized in order to identify the strains which are interesting for a further investigation. Several techniques have been developed for identifying biosurfactant producing strains. Most of them are directly based on the surface or interfacial activity of the culture supernatant. Apart from that, some screening methods explore the hydrophobicity of the cell surface. This trait also gives an indication on biosurfactant production. In recent years automation and miniaturization have led to the development of high throughput methods for screening. High throughput screening (HTS) for analyzing large amounts of potential candidates or whole culture collections is reflected in the end. However, no new principals have been introduced by HTS methods.

  18. Optimizing kaon production

    International Nuclear Information System (INIS)

    Berley, D.

    1976-01-01

    Kaon production data are examined with the view toward producing high quality beams in the future. Emphasis is placed upon the low momentum region, below 1 GeV/c, where the behavior of the kaon momentum spectrum is shown to be dominated by strongly momentum dependent kinematic factors. No complete experimental survey of production cross sections at different incident proton energies exists from a single apparatus. Information on relative production yields for different proton energies is important in the choice of parameters for an accelerator constructed primarily as a source of kaons. A summary is presented of existing kaon production facilities in the United States. These are all at proton accelerators. The possibility of using electron accelerators as kaon sources is examined. It is found that secondary beam fluxes can be contained at electron linacs, which are comparable to those obtained at proton synchrotrons. All kaon beam transport systems presently in use employ quadrupole and dipole magnet elements. A superconducting solenoid transport system has the potential advantage of much larger solid angle acceptance than the familiar quadrupole system. The possibility of mass separation by the differential energy loss method appears adaptable to the solenoid system

  19. Optimal taxation with home production

    OpenAIRE

    Olovsson, Conny

    2014-01-01

    Optimal taxes for Europe and the U.S. are derived in a realistically calibrated model in which agents buy consumption goods and services and use home capital and labor to produce household services. The optimal tax rate on services is substantially lower than the tax rate on goods. Specifically, the planner cannot tax home production directly and instead lowers the tax rate on market services to increase the relative price of home production. The optimal tax rate on the return to home capital...

  20. Properties of a biosurfactant produced by Bacillus pumilus using vinasse and waste frying oil as alternative carbon sources

    Directory of Open Access Journals (Sweden)

    Juliana Guerra de Oliveira

    2013-02-01

    Full Text Available Biosurfactants are chemical molecules produced by the microorganisms with potential for application in various industrial and environmental sectors. The production parameters and the physicochemical properties of a biosurfactant synthesized by Bacillus pumilus using different concentrations of vinasse and waste frying oil as alternative carbon sources were analyzed. The microorganism was able to grow and produce a biosurfactant using both the residues. The surface tension was reduced up to 45 mN/m and the maximum production of crude biosurfactant was 27.7 and 5.7 g/l for vinasse and waste frying oil, respectively, in concentration of 5%. The critical micelle concentration (CMC results of 1.5 and 0.2 g/l showed the efficiency of the biosurfactant produced on both the substrates. The results showed that the alternative substrates could be used for the production of an efficient biosurfactant by B. pumilus. These properties have potential for industrial and environmental applications.

  1. Improved bioavailability and biodegradation of a model polyaromatic hydrocarbon by a biosurfactant producing bacterium of marine origin.

    Science.gov (United States)

    Das, Palashpriya; Mukherjee, Soumen; Sen, Ramkrishna

    2008-07-01

    Polyaromatic hydrocarbons (PAHs) are organic pollutants mostly derived from the processing and combustion of fossil fuels and cause human health hazards. In the present study a marine biosurfactant producing strain of Bacillus circulans was used to increase the bioavailability and consequent degradation of a model polyaromatic hydrocarbon, anthracene. Although the organism could not utilize anthracene as the sole carbon source, it showed better growth and biosurfactant production in an anthracene supplemented glycerol mineral salts medium (AGlyMSM) compared to a normal glycerol mineral salts medium (GlyMSM). The biosurfactant product showed high degree of emulsification of various hydrocarbons. Analysis by gas chromatography (GC), high performance thin layer chromatography (HPTLC) and Fourier transform infrared spectroscopy (FTIR) showed that the biosurfactant could effectively entrap and solubilize PAH. Thin layer chromatographic analysis showed that anthracene was utilized as a carbon substrate for the production of biosurfactant. Thus organic pollutant anthracene was metabolized and converted to biosurfactants facilitating its own bioremediation.

  2. Biosurfactants: Multifunctional Biomolecules of the 21st Century.

    Science.gov (United States)

    Santos, Danyelle Khadydja F; Rufino, Raquel D; Luna, Juliana M; Santos, Valdemir A; Sarubbo, Leonie A

    2016-03-18

    In the era of global industrialisation, the exploration of natural resources has served as a source of experimentation for science and advanced technologies, giving rise to the manufacturing of products with high aggregate value in the world market, such as biosurfactants. Biosurfactants are amphiphilic microbial molecules with hydrophilic and hydrophobic moieties that partition at liquid/liquid, liquid/gas or liquid/solid interfaces. Such characteristics allow these biomolecules to play a key role in emulsification, foam formation, detergency and dispersal, which are desirable qualities in different industries. Biosurfactant production is considered one of the key technologies for development in the 21st century. Besides exerting a strong positive impact on the main global problems, biosurfactant production has considerable importance to the implantation of sustainable industrial processes, such as the use of renewable resources and "green" products. Biodegradability and low toxicity have led to the intensification of scientific studies on a wide range of industrial applications for biosurfactants in the field of bioremediation as well as the petroleum, food processing, health, chemical, agricultural and cosmetic industries. In this paper, we offer an extensive review regarding knowledge accumulated over the years and advances achieved in the incorporation of biomolecules in different industries.

  3. Biosurfactants: Multifunctional Biomolecules of the 21st Century

    Directory of Open Access Journals (Sweden)

    Danyelle Khadydja F. Santos

    2016-03-01

    Full Text Available In the era of global industrialisation, the exploration of natural resources has served as a source of experimentation for science and advanced technologies, giving rise to the manufacturing of products with high aggregate value in the world market, such as biosurfactants. Biosurfactants are amphiphilic microbial molecules with hydrophilic and hydrophobic moieties that partition at liquid/liquid, liquid/gas or liquid/solid interfaces. Such characteristics allow these biomolecules to play a key role in emulsification, foam formation, detergency and dispersal, which are desirable qualities in different industries. Biosurfactant production is considered one of the key technologies for development in the 21st century. Besides exerting a strong positive impact on the main global problems, biosurfactant production has considerable importance to the implantation of sustainable industrial processes, such as the use of renewable resources and “green” products. Biodegradability and low toxicity have led to the intensification of scientific studies on a wide range of industrial applications for biosurfactants in the field of bioremediation as well as the petroleum, food processing, health, chemical, agricultural and cosmetic industries. In this paper, we offer an extensive review regarding knowledge accumulated over the years and advances achieved in the incorporation of biomolecules in different industries.

  4. Biosurfactant-producing bacterium, Pseudomonas aeruginosa MA01 isolated from spoiled apples: physicochemical and structural characteristics of isolated biosurfactant.

    Science.gov (United States)

    Abbasi, Habib; Hamedi, Mir Manochehr; Lotfabad, Tayebe Bagheri; Zahiri, Hossein Shahbani; Sharafi, Hakimeh; Masoomi, Fatemeh; Moosavi-Movahedi, Ali Akbar; Ortiz, Antonio; Amanlou, Massoud; Noghabi, Kambiz Akbari

    2012-02-01

    An extensive investigation was conducted to isolate indigenous bacterial strains with outstanding performance for biosurfactant production from different types of spoiled fruits, food-related products and food processing industries. An isolate was selected from 800 by the highest biosurfactant yield in soybean oil medium and it was identified by 16S rRNA and the two most relevant hypervariable regions of this gene; V3 and V6 as Pseudomonas aeruginosa MA01. The isolate was able to produce 12 g/l of a glycolipid-type biosurfactant and generally less efficient to emulsify vegetable oils compared to hydrocarbons and could emulsify corn and coconut oils more than 50%. However, emulsification index (E(24)) of different hydrocarbons including hexane, toluene, xylene, brake oil, kerosene and hexadecane was between 55.8% and 100%. The surface tension of pure water decreased gradually with increasing biosurfactant concentration to 32.5 mNm(-1) with critical micelle concentration (CMC) value of 10.1mg/l. Among all carbon substrates examined, vegetable oils were the most effective on biosurfactant production. Two glycolipid fractions were purified from the biosurfactant crude extracts, and FTIR and ES-MS were used to determine the structure of these compounds. The analysis indicated the presence of three major monorhamnolipid species: R(1)C(10)C(10), R(1)C(10)C(12:1), and R(1)C(10)C(12); as well as another three major dirhamnolipid species: R(2)C(10)C(10), R(2)C(10)C(12:1), and R(2)C(10)C(12). The strain sweep experiment for measuring the linear viscoelastic of biosurfactant showed that typical behavior characteristics of a weak viscoelastic gel, with storage modulus greater than loss modulus at all frequencies examined, both showing some frequency dependence. Copyright © 2011 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  5. Rhamnolipid biosurfactants: evolutionary implications, applications and future prospects from untapped marine resource.

    Science.gov (United States)

    Kiran, George Seghal; Ninawe, Arun Shivanth; Lipton, Anuj Nishanth; Pandian, Vijayalakshmi; Selvin, Joseph

    2016-01-01

    Rhamnolipid-biosurfactants are known to be produced by the genus Pseudomonas, however recent literature reported that rhamnolipids (RLs) are distributed among diverse microbial genera. To integrate the evolutionary implications of rhamnosyl transferase among various groups of microorganisms, a comprehensive comparative motif analysis was performed amongst bacterial producers. Findings on new RL-producing microorganism is helpful from a biotechnological perspective and to replace infective P. aeruginosa strains which ultimately ensure industrially safe production of RLs. Halotolerant biosurfactants are required for efficient bioremediation of marine oil spills. An insight on the exploitation of marine microbes as the potential source of RL biosurfactants is highlighted in the present review. An economic production process, solid-state fermentation using agro-industrial and industrial waste would increase the scope of biosurfactants commercialization. Potential and prospective applications of RL-biosurfactants including hydrocarbon bioremediation, heavy metal removal, antibiofilm activity/biofilm disruption and greener synthesis of nanoparticles are highlighted in this review.

  6. Individually and Synergistic Degradation of Hydrocarbons by Biosurfactant Producing Bacteria

    Directory of Open Access Journals (Sweden)

    Amirarsalan Kavyanifard

    2016-02-01

    Full Text Available Background: Increasing worldwide contamination with hydrocarbons has urged environmental remediation using biological agents such as bacteria. Our goal here was to study the phylogenetic relationship of two crude oil degrader bacteria and investigation of their ability to degrade hydrocarbons. Materials and Methods: Phylogenetic relationship of isolates was determined using morphological and biochemical characteristics and 16S rDNA gene sequencing. Optimum conditions of each isolate for crude oil degradation were investigated using one factor in time method. The rate of crude oil degradation by individual and consortium bacteria was assayed via Gas chromatography–mass spectrometry (GC-MS analysis. Biosurfactant production was measured by Du Noüy ring method using Krüss-K6 tensiometer. Results: The isolates were identified as Dietzia cinnamea KA1 and Dietzia cinnamea AP and clustered separately, while both are closely related to each other and with other isolates of Dietzia cinnamea. The optimal conditions for D. cinnamea KA1 were 35°C, pH9.0, 510 mM NaCl, and minimal requirement of 46.5 mM NH4Cl and 2.10 mM NaH2PO4. In the case of D. cinnamea AP, the values were 30°C, pH8.0, 170 mM NaCl, and minimal requirement of 55.8 mM NH4Cl and 2.10 mM NaH2PO4, respectively. Gas chromatography – Mass Spectroscopy (GC-MS analysis showed that both isolates were able to utilize various crude oil compounds, but D. cinnamea KA1 was more efficient individually and consortium of isolates was the most. The isolates were able to grow and produce biosurfactant when cultured in MSM supplemented with crude oil and optimization of MSM conditions lead to increase in biosurfactant production. Conclusion: To the best of our knowledge this is the first report of synergistic relationship between two strains of D. cinnamea in biodegradation of crude oil components, including poisonous and carcinogenic compound in a short time.

  7. Isolation and selection of new biosurfactant producing bacteria from degraded palm kernel cake under liquid state fermentation.

    Science.gov (United States)

    Jamal, Parveen; Mir, Shajrat; Alam, Md Zahangir; Wan Nawawi, Wan M Fazli

    2014-01-01

    Biosurfactants are surface-active compounds produced by different microorganisms. The aim of this study was to introduce palm kernel cake (PKC) as a novel substrate for biosurfactant production using a potent bacterial strain under liquid state fermentation. This study was primarily based on the isolation and identification of biosurfactant-producing bacteria that could utilize palm kernel cake as a new major substrate. Potential bacterial strains were isolated from degraded PKC and screened for biosurfactant production with the help of the drop collapse assay and by analyzing the surface tension activity. From the screened isolates, a new strain, SM03, showed the best and most consistent results, and was therefore selected as the most potent biosurfactant-producing bacterial strain. The new strain was identified as Providencia alcalifaciens SM03 using the Gen III MicroPlate Biolog Microbial Identification System. The yield of the produced biosurfactant was 8.3 g/L.

  8. Biosurfactant and enzyme mediated crude oil degradation by Pseudomonas stutzeri NA3 and Acinetobacter baumannii MN3.

    Science.gov (United States)

    Parthipan, Punniyakotti; Elumalai, Punniyakotti; Sathishkumar, Kuppusamy; Sabarinathan, Devaraj; Murugan, Kadarkarai; Benelli, Giovanni; Rajasekar, Aruliah

    2017-10-01

    The present study focuses on the optimization of biosurfactant (BS) production using two potential biosurfactant producer Pseudomonas stutzeri NA3 and Acinetobacter baumannii MN3 and role of enzymes in the biodegradation of crude oil. The optimal conditions for P. stutzeri NA3 and A. baumannii MN3 for biodegradation were pH of 8 and 7; temperature of 30 and 40 °C, respectively. P. stutzeri NA3 and A. baumannii MN3 produced 3.81 and 4.68 g/L of BS, respectively. Gas chromatography mass spectrometry confirmed that BS was mainly composed of fatty acids. Furthermore, the role of the degradative enzymes, alkane hydroxylase, alcohol dehydrogenase and laccase on biodegradation of crude oil are explained. Maximum biodegradation efficiency (BE) was recorded for mixed consortia (86%) followed by strain P. stutzeri NA3 (84%). Both bacterial strains were found to be vigorous biodegraders of crude oil than other biosurfactant-producing bacteria due to their enzyme production capabilities and our results suggests that the bacterial isolates can be used for effective degradation of crude oil within short time periods.

  9. Co-produção de lipase e biossurfactante em estado sólido para utilização em biorremediação de óleos vegetais e hidrocarbonetos Lipases and biosurfactant production by solid state fermentation for utilization in bioremediation of vegetable oils and hydrocarbons

    Directory of Open Access Journals (Sweden)

    Vilásia Guimarães Martins

    2008-01-01

    Full Text Available Recently lipases have been increasing in prominence due to its wide industrial application. The lipase production can be influenced by different variables such as the producing microorganism, carbon sources, aeration and agitation conditions, inductor type and the geometry of the reactor. Biosurfactants are composites of surface active produced by microbial cells which reduce superficial and interfacial tensions. The objective of this study was to verify the influence of different process variables in the lipase production during a fermentative process. The results showed that the concomitant production of lipases and biosurfactant was possible in different cultivation conditions.

  10. Ex situ treatment of hydrocarbon-contaminated soil using biosurfactants from Lactobacillus pentosus.

    Science.gov (United States)

    Moldes, Ana Belén; Paradelo, Remigio; Rubinos, David; Devesa-Rey, Rosa; Cruz, José Manuel; Barral, María Teresa

    2011-09-14

    The utilization of biosurfactants for the bioremediation of contaminated soil is not yet well established, because of the high production cost of biosurfactants. Consequently, it is interesting to look for new biosurfactants that can be produced at a large scale, and it can be employed for the bioremediation of contaminated sites. In this work, biosurfactants from Lactobacillus pentosus growing in hemicellulosic sugars solutions, with a similar composition of sugars found in trimming vine shoot hydrolysates, were employed in the bioremediation of soil contaminated with octane. It was observed that the presence of biosurfactant from L. pentosus accelerated the biodegradation of octane in soil. After 15 days of treatment, biosurfactants from L. pentosus reduced the concentration of octane in the soil to 58.6 and 62.8%, for soil charged with 700 and 70,000 mg/kg of hydrocarbon, respectively, whereas after 30 days of treatment, 76% of octane in soil was biodegraded in both cases. In the absence of biosurfactant and after 15 days of incubation, only 1.2 and 24% of octane was biodegraded in soil charged with 700 and 70,000 mg/kg of octane, respectively. Thus, the use of biosurfactants from L. pentosus, as part of a well-designed bioremediation process, can provide mechanisms to mobilize the target contaminants from the soil surface to make them more available to the microbial population.

  11. Synthesis of rhamnolipid biosurfactant and mode of hexadecane uptake by Pseudomonas species

    Directory of Open Access Journals (Sweden)

    Singh Pooja

    2009-03-01

    Full Text Available Abstract Background Microorganisms have devised ways by which they increase the bioavailability of many water immiscible substrates whose degradation rates are limited by their low water solubility. Hexadecane is one such water immiscible hydrocarbon substrate which forms an important constituent of oil. One major mechanism employed by hydrocarbon degrading organisms to utilize such substrates is the production of biosurfactants. However, much of the overall mechanism by which such organisms utilize hydrocarbon substrate still remains a mystery. Results With an aim to gain more insight into hydrocarbon uptake mechanism, an efficient biosurfactant producing and n-hexadecane utilizing Pseudomonas sp was isolated from oil contaminated soil which was found to produce rhamnolipid type of biosurfactant containing a total of 13 congeners. Biosurfactant action brought about the dispersion of hexadecane to droplets smaller than 0.22 μm increasing the availability of the hydrocarbon to the degrading organism. Involvement of biosurfactant was further confirmed by electron microscopic studies. Biosurfactant formed an emulsion with hexadecane thereby facilitating increased contact between hydrocarbon and the degrading bacteria. Interestingly, it was observed that "internalization" of "biosurfactant layered hydrocarbon droplet" was taking place suggesting a mechanism similar in appearance to active pinocytosis, a fact not earlier visually reported in bacterial systems for hydrocarbon uptake. Conclusion This study throws more light on the uptake mechanism of hydrocarbon by Pseudomonas aeruginosa. We report here a new and exciting line of research for hydrocarbon uptake involving internalization of biosurfactant covered hydrocarbon inside cell for subsequent breakdown.

  12. Study on mechanisms of biosurfactant-enhanced composting technology for waste management

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, B.Y.; Huang, G.H.; Chen, B.; Xi, B.D.; Maqsood, I. [Regina Univ., SK (Canada)

    2003-07-01

    Composting is increasingly being used for solid waste treatment. The efficiency of solid waste composting might be enhanced using biosurfactants produced by microbial activities. This study was conducted to characterize the effect of biosurfactant on solid waste biodegradation throughout the composting process. The method employed involves shredding solid waste, followed by a treatment in an 8-litre (L) batch reactor. Biosurfactant production was monitored daily along with characteristics and maturity degree. Surface tension and emulsification capacity were of particular concern. The measurement of indices such as humic acid carbon (CHA) and fulvic acid carbon (CFA) were used to evaluate the maturity degree. The results indicated that the highest level of biosurfactant concentration was achieved on the third day, and within two days, related emulsification capacity reached its peak. This study confirmed the presence of biosurfactants and their function during the composting process. 16 refs., 2 tabs., 4 figs.

  13. DEVELOPMENT OF MICROORGANISMS WITH IMPROVED TRANSPORT AND BIOSURFACTANT ACTIVITY FOR ENHANCED OIL RECOVERY

    Energy Technology Data Exchange (ETDEWEB)

    M.J. McInerney; N. Youssef; T. Fincher; S.K. Maudgalya; M.J. Folmsbee; R. Knapp; D. Nagle

    2004-05-31

    Diverse microorganisms were screened for biosurfactant production and anaerobic growth at elevated salt concentrations to obtain candidates most suitable for microbial oil recovery. Seventy percent of the 205 strains tested, mostly strains of Bacillus mojavensis, Bacillus subtilis, Bacillus licheniformis, and Bacillus sonorensis, produced biosurfactants aerobically and 41% of the strains had biosurfactant activity greater than Bacillus mojavensis JF-2, the current candidate for oil recovery. Biosurfactant activity varied with the percentage of the 3-hydroxy-tetradecanoate isomers in the fatty acid portion of the biosurfactant. Changing the medium composition by incorporation of different precursors of 3-hydroxy tetradecanoate increased the activity of biosurfactant. The surface tension and critical micelle concentration of 15 different, biosurfactant-producing Bacillus strains was determined individually and in combination with other biosurfactants. Some biosurfactant mixtures were found to have synergistic effect on surface tension (e.g. surface tension was lowered from 41 to 31 mN/m in some cases) while others had a synergistic effect on CMD-1 values. We compared the transport abilities of spores from three Bacillus strains using a model porous system to study spore recovery and transport. Sand-packed columns were used to select for spores or cells with the best transport abilities through brine-saturated sand. Spores of Bacillus mojavensis strains JF-2 and ROB-2 and a natural recombinant, strain C-9, transported through sand at very high efficiencies. The earliest cells/spores that emerged from the column were re-grown, allowed to sporulate, and applied to a second column. This procedure greatly enhanced the transport of strain C-9. Spores with enhanced transport abilities can be easily obtained and that the preparation of inocula for use in MEOR is feasible. Tertiary oil recovery experiments showed that 10 to 40 mg/l of JF-2 biosurfactant in the presence of 0

  14. Oil Reservoir Production Optimization using Optimal Control

    DEFF Research Database (Denmark)

    Völcker, Carsten; Jørgensen, John Bagterp; Stenby, Erling Halfdan

    2011-01-01

    Practical oil reservoir management involves solution of large-scale constrained optimal control problems. In this paper we present a numerical method for solution of large-scale constrained optimal control problems. The method is a single-shooting method that computes the gradients using the adjo...... reservoir using water ooding and smart well technology. Compared to the uncontrolled case, the optimal operation increases the Net Present Value of the oil field by 10%.......Practical oil reservoir management involves solution of large-scale constrained optimal control problems. In this paper we present a numerical method for solution of large-scale constrained optimal control problems. The method is a single-shooting method that computes the gradients using...

  15. Functional, genetic and chemical characterization of biosurfactants produced by plant growth-promoting Pseudomonas putida 267.

    Science.gov (United States)

    Kruijt, Marco; Tran, Ha; Raaijmakers, Jos M

    2009-08-01

    Plant growth-promoting Pseudomonas putida strain 267, originally isolated from the rhizosphere of black pepper, produces biosurfactants that cause lysis of zoospores of the oomycete pathogen Phytophthora capsici. The biosurfactants were characterized, the biosynthesis gene(s) partially identified, and their role in control of Phytophthora damping-off of cucumber evaluated. The biosurfactants were shown to lyse zoospores of Phy. capsici and inhibit growth of the fungal pathogens Botrytis cinerea and Rhizoctonia solani. In vitro assays further showed that the biosurfactants of strain 267 are essential in swarming motility and biofilm formation. In spite of the zoosporicidal activity, the biosurfactants did not play a significant role in control of Phytophthora damping-off of cucumber, since both wild type strain 267 and its biosurfactant-deficient mutant were equally effective, and addition of the biosurfactants did not provide control. Genetic characterization revealed that surfactant biosynthesis in strain 267 is governed by homologues of PsoA and PsoB, two nonribosomal peptide synthetases involved in production of the cyclic lipopeptides (CLPs) putisolvin I and II. The structural relatedness of the biosurfactants of strain 267 to putisolvins I and II was supported by LC-MS and MS-MS analyses. The biosurfactants produced by Ps. putida 267 were identified as putisolvin-like CLPs; they are essential in swarming motility and biofilm formation, and have zoosporicidal and antifungal activities. Strain 267 provides excellent biocontrol activity against Phytophthora damping-off of cucumber, but the lipopeptide surfactants are not involved in disease suppression. Pseudomonas putida 267 suppresses Phy. capsici damping-off of cucumber and provides a potential supplementary strategy to control this economically important oomycete pathogen. The putisolvin-like biosurfactants exhibit zoosporicidal and antifungal activities, yet they do not contribute to biocontrol of Phy

  16. Development of More Effective Biosurfactants for Enhanced Oil Recovery/Advanced Recovery Concepts Awards; SEMIANNUAL

    International Nuclear Information System (INIS)

    McInerney, M.J.; Marsh, T.L.; Zhang, X.; Knapp, R.M.; Nagle, Jr. D.P.; Sharma, P.K.; Jackson, B.E.

    2002-01-01

    The objectives of this were two fold. First, core displacement studies were done to determine whether microbial processes could recover residual oil at elevated pressures. Second, the importance of biosurfactant production for the recovery of residual oil was studies. In these studies, a biosurfactant-producing, microorganisms called Bacillus licheniformis strain JF-2 was used. This bacterium produces a cyclic peptide biosurfactant that significantly reduces the interfacial tension between oil and brine (7). The use of a mutant deficient in surfactant production and a mathematical MEOR simulator were used to determine the major mechanisms of oil recovery by these two strains

  17. Development of More Effective Biosurfactants for Enhanced Oil Recovery/Advanced Recovery Concepts Awards

    Energy Technology Data Exchange (ETDEWEB)

    McInerney, M.J.; Marsh, T.L.; Zhang, X.; Knapp, R.M.; Nagle, Jr., D.P.; Sharma, P.K.; Jackson, B.E.

    2002-05-28

    The objectives of this were two fold. First, core displacement studies were done to determine whether microbial processes could recover residual oil at elevated pressures. Second, the importance of biosurfactant production for the recovery of residual oil was studies. In these studies, a biosurfactant-producing, microorganisms called Bacillus licheniformis strain JF-2 was used. This bacterium produces a cyclic peptide biosurfactant that significantly reduces the interfacial tension between oil and brine (7). The use of a mutant deficient in surfactant production and a mathematical MEOR simulator were used to determine the major mechanisms of oil recovery by these two strains.

  18. Optimizing clinical drug product performance

    DEFF Research Database (Denmark)

    Dickinson, Paul A.; Kesisoglou, Filippos; Flanagan, Talia

    2016-01-01

    The aim of Biopharmaceutics Risk Assessment Roadmap (BioRAM) and the BioRAM Scoring Grid is to facilitate optimization of clinical performance of drug products. BioRAM strategy relies on therapy-driven drug delivery and follows an integrated systems approach for formulating and addressing critical...... questions and decision-making (J Pharm Sci. 2014,103(11): 3777-97). In BioRAM, risk is defined as not achieving the intended in vivo drug product performance, and success is assessed by time to decision-making and action. Emphasis on time to decision-making and time to action highlights the value of well....... Application of the BioRAM Scoring Grid is illustrated using published literature. Organizational considerations for implementing BioRAM strategy, including the interactions, function, and skillsets of the BioRAM group members, are also reviewed. As a creative and innovative systems approach, we believe...

  19. Screening of biosurfactant-producing Bacillus strains using glycerol from the biodiesel synthesis as main carbon source.

    Science.gov (United States)

    Sousa, M; Melo, V M M; Rodrigues, S; Sant'ana, H B; Gonçalves, L R B

    2012-08-01

    Glycerol, a co-product of biodiesel production, was evaluated as carbon source for biosurfactant production. For this reason, seven non-pathogenic biosurfactant-producing Bacillus strains, isolated from the tank of chlorination at the Wastewater Treatment Plant at Federal University of Ceara, were screened. The production of biosurfactant was verified by determining the surface tension value, as well as the emulsifying capacity of the free-cell broth against soy oil, kerosene and N-hexadecane. Best results were achieved when using LAMI005 and LAMI009 strains, whose biosurfactant reduced the surface tension of the broth to 28.8 ± 0.0 and 27.1 ± 0.1 mN m(-1), respectively. Additionally, at 72 h of cultivation, 441.06 and 267.56 mg L(-1) of surfactin were produced by LAMI005 and LAMI009, respectively. The biosurfactants were capable of forming stable emulsions with various hydrocarbons, such as soy oil and kerosene. Analyses carried out with high performance liquid chromatography (HPLC) showed that the biosurfactant produced by Bacillus subtilis LAMI009 and LAMI005 was compatible with the commercially available surfactin standard. The values of minimum surface tension and the CMC of the produced biosurfactant indicated that it is feasible to produce biosurfactants from a residual and renewable and low-cost carbon source, such as glycerol.

  20. Optimizing biodiesel production in India

    International Nuclear Information System (INIS)

    Leduc, Sylvain; Natarajan, Karthikeyan; McCallum, Ian; Obersteiner, Michael; Dotzauer, Erik

    2009-01-01

    India is expected to at least double its fuel consumption in the transportation sector by 2030. To contribute to the fuel supply, renewable energies such as jatropha appear to be an attractive resource for biodiesel production in India as it can be grown on waste land and does not need intensive water supply. In order to produce biodiesel at a competitive cost, the biodiesel supply chain - from biomass harvesting to biodiesel delivery to the consumers - is analyzed. A mixed integer linear programming model is used in order to determine the optimal number and geographic locations of biodiesel plants. The optimization is based on minimization of the costs of the supply chain with respect to the biomass, production and transportation costs. Three biodiesel blends are considered, B2, B5 and B10. For each blend, 13 scenarios are considered where yield, biomass cost, cake price, glycerol price, transport cost and investment costs are studied. A sensitivity analysis is carried out on both those parameters and the resulting locations of the plants. The emissions of the supply chain are also considered. The results state that the biomass cost has most influence on the biodiesel cost (an increase of feedstock cost increases the biodiesel cost by about 40%) and to a lower effect, the investment cost and the glycerol price. Moreover, choosing the right set of production plant locations highly depends on the scenarios that have the highest probability to occur, for which the production plant locations still produce a competitive biodiesel cost and emissions from the transportation are minimum. In this study, one set of plant locations happened to meet these two requirements. (author)

  1. Environmental applications of biosurfactants: recent advances.

    Science.gov (United States)

    Pacwa-Płociniczak, Magdalena; Płaza, Grażyna A; Piotrowska-Seget, Zofia; Cameotra, Swaranjit Singh

    2011-01-18

    Increasing public awareness of environmental pollution influences the search and development of technologies that help in clean up of organic and inorganic contaminants such as hydrocarbons and metals. An alternative and eco-friendly method of remediation technology of environments contaminated with these pollutants is the use of biosurfactants and biosurfactant-producing microorganisms. The diversity of biosurfactants makes them an attractive group of compounds for potential use in a wide variety of industrial and biotechnological applications. The purpose of this review is to provide a comprehensive overview of advances in the applications of biosurfactants and biosurfactant-producing microorganisms in hydrocarbon and metal remediation technologies.

  2. Environmental Applications of Biosurfactants: Recent Advances

    Directory of Open Access Journals (Sweden)

    Swaranjit Singh Cameotra

    2011-01-01

    Full Text Available Increasing public awareness of environmental pollution influences the search and development of technologies that help in clean up of organic and inorganic contaminants such as hydrocarbons and metals. An alternative and eco-friendly method of remediation technology of environments contaminated with these pollutants is the use of biosurfactants and biosurfactant-producing microorganisms. The diversity of biosurfactants makes them an attractive group of compounds for potential use in a wide variety of industrial and biotechnological applications. The purpose of this review is to provide a comprehensive overview of advances in the applications of biosurfactants and biosurfactant-producing microorganisms in hydrocarbon and metal remediation technologies.

  3. Biosurfactant Producing Microbes from Oil Contaminated Soil - Isolation, Screening and Characterization

    OpenAIRE

    , A Pandey; , D Nandi; , N Prasad; , S Arora

    2016-01-01

    Th1s paper bas1cally deals W1th 1solat10n, productıon and characterızatıon of biosurfactant producing microbes from oil contaminated soil sample. In this paper, we are comparing and discussing different methods to screen & characterize microbes from soil which can degrade oil due to their biosurfactant producing activity which helps in reduction of surface tension of oil. Oils used to check the biosurfactant activity of microbes, were engine oil and vegetable oil. Further isolation of...

  4. Potential therapeutic applications of biosurfactants.

    Science.gov (United States)

    Gudiña, Eduardo J; Rangarajan, Vivek; Sen, Ramkrishna; Rodrigues, Lígia R

    2013-12-01

    Biosurfactants have recently emerged as promising molecules for their structural novelty, versatility, and diverse properties that are potentially useful for many therapeutic applications. Mainly due to their surface activity, these molecules interact with cell membranes of several organisms and/or with the surrounding environments, and thus can be viewed as potential cancer therapeutics or as constituents of drug delivery systems. Some types of microbial surfactants, such as lipopeptides and glycolipids, have been shown to selectively inhibit the proliferation of cancer cells and to disrupt cell membranes causing their lysis through apoptosis pathways. Moreover, biosurfactants as drug delivery vehicles offer commercially attractive and scientifically novel applications. This review covers the current state-of-the-art in biosurfactant research for therapeutic purposes, providing new directions towards the discovery and development of molecules with novel structures and diverse functions for advanced applications. Copyright © 2013 Elsevier Ltd. All rights reserved.

  5. Sophorolipids Production by Candida bombicola ATCC 22214 and its Potential Application in Microbial Enhanced Oil Recovery.

    Science.gov (United States)

    Elshafie, Abdulkadir E; Joshi, Sanket J; Al-Wahaibi, Yahya M; Al-Bemani, Ali S; Al-Bahry, Saif N; Al-Maqbali, Dua'a; Banat, Ibrahim M

    2015-01-01

    Biosurfactant production using Candida bombicola ATCC 22214, its characterization and potential applications in enhancing oil recovery were studied at laboratory scale. The seed media and the production media were standardized for optimal growth and biosurfactant production. The production media were tested with different carbon sources: glucose (2%w/v) and corn oil (10%v/v) added separately or concurrently. The samples were collected at 24 h interval up to 120 h and checked for growth (OD660), and biosurfactant production [surface tension (ST) and interfacial tension (IFT)]. The medium with both glucose and corn oil gave better biosurfactant production and reduced both ST and IFT to 28.56 + 0.42mN/m and 2.13 + 0.09mN/m, respectively within 72 h. The produced biosurfactant was quite stable at 13-15% salinity, pH range of 2-12, and at temperature up to 100°C. It also produced stable emulsions (%E24) with different hydrocarbons (pentane, hexane, heptane, tridecane, tetradecane, hexadecane, 1-methylnaphthalene, 2,2,4,4,6,8-heptamethylnonane, light and heavy crude oil). The produced biosurfactant was extracted using ethyl acetate and characterized as a mixture of sophorolipids (SPLs). The potential of SPLs in enhancing oil recovery was tested using core-flooding experiments under reservoir conditions, where additional 27.27% of residual oil (Sor) was recovered. This confirmed the potential of SPLs for applications in microbial enhanced oil recovery.

  6. Development of Microorganisms with Improved Transport and Biosurfactant Activity for Enhanced Oil Recovery

    Energy Technology Data Exchange (ETDEWEB)

    M.J. McInerney; K.E. Duncan; N. Youssef; T. Fincher; S.K. Maudgalya; M.J. Folmsbee; R. Knapp; Randy R. Simpson; N.Ravi; D. Nagle

    2005-08-15

    The project had three objectives: (1) to develop microbial strains with improved biosurfactant properties that use cost-effective nutrients, (2) to obtain biosurfactant strains with improved transport properties through sandstones, and (3) to determine the empirical relationship between surfactant concentration and interfacial tension and whether in situ reactions kinetics and biosurfactant concentration meets appropriate engineering design criteria. Here, we show that a lipopeptide biosurfactant produced by Bacillus mojavensis strain JF-2 mobilized substantial amounts of residual hydrocarbon from sand-packed columns and Berea sandstone cores when a viscosifying agent and a low molecular weight alcohol were present. The amount of residual hydrocarbon mobilized depended on the biosurfactant concentration. Tertiary oil recovery experiments showed that 10 to 40 mg/l of JF-2 biosurfactant in the presence of 0.1 mM 2,3-butanediol and 1 g/l of partially hydrolyzed polyacrylamide (PHPA) recovered 10-40% of residual oil from Berea sandstone cores. Even low biosurfactant concentrations (16 mg/l) mobilized substantial amounts of residual hydrocarbon (29%). The bio-surfactant lowered IFT by nearly 2 orders of magnitude compared to typical IFT values of 28-29 mN/m. Increasing the salinity increased the IFT with or without 2,3-butanediol present. The lowest interfacial tension observed was 0.1 mN/m. A mathematical model that relates oil recovery to biosurfactant concentration was modified to include the stepwise changes in IFT as biosurfactant concentrations changes. This model adequately predicted the experimentally observed changes in IFT as a function of biosurfactant concentration. Theses data show that lipopeptide biosurfactant systems may be effective in removing hydrocarbon contamination sources in soils and aquifers and for the recovery of entrapped oil from low production oil reservoirs. Diverse microorganisms were screened for biosurfactant production and anaerobic

  7. DEVELOPMENT OF MICROORGANISMS WITH IMPROVED TRANSPORT AND BIOSURFACTANT ACTIVITY FOR ENHANCED OIL RECOVERY

    Energy Technology Data Exchange (ETDEWEB)

    M.J. McInerney; R.M. Knapp; D.P. Nagle, Jr.; Kathleen Duncan; N. Youssef; M.J. Folmsbee; S. Maudgakya

    2003-06-26

    Biosurfactants enhance hydrocarbon biodegradation by increasing apparent aqueous solubility or affecting the association of the cell with poorly soluble hydrocarbon. Here, we show that a lipopeptide biosurfactant produced by Bacillus mojavensis strain JF-2 mobilized substantial amounts of residual hydrocarbon from sand-packed columns when a viscosifying agent and a low molecular weight alcohol were present. The amount of residual hydrocarbon mobilized depended on the biosurfactant concentration. One pore volume of cell-free culture fluid with 900 mg/l of the biosurfactant, 10 mM 2,3-butanediol and 1000 mg/l of partially hydrolyzed polyacrylamide polymer mobilized 82% of the residual hydrocarbon. Consistent with the high residual oil recoveries, we found that the bio-surfactant lowered the interfacial tension (IFT) between oil and water by nearly 2 orders of magnitude compared to typical IFT values of 28-29 mN/m. Increasing the salinity increased the IFT with or without 2,3-butanediol present. The lowest interfacial tension observed was 0.1 mN/m. The lipopeptide biosurfactant system may be effective in removing hydrocarbon contamination sources in soils and aquifers and for the recovery of entrapped oil from low production oil reservoirs. Previously, we reported that Proteose peptone was necessary for anaerobic growth and biosurfactant production by B. mojavensis JF-2. The data gathered from crude purification of the growth-enhancing factor in Proteose peptone suggested that it consisted of nucleic acids; however, nucleic acid bases, nucleotides or nucleosides did not replace the requirement for Proteose Peptone. Further studies revealed that salmon sperm DNA, herring sperm DNA, Echerichia coli DNA and synthetic DNA replaced the requirement for Proteose peptone. In addition to DNA, amino acids and nitrate were required for anaerobic growth and vitamins further improved growth. We now have a defined medium that can be used to manipulate growth and biosurfactant

  8. A model for optimizing the production of pharmaceutical products

    Directory of Open Access Journals (Sweden)

    Nevena Gospodinova

    2017-05-01

    Full Text Available The problem associated with the optimal production planning is especially relevant in modern industrial enterprises. The most commonly used optimality criteria in this context are: maximizing the total profit; minimizing the cost per unit of production; maximizing the capacity utilization; minimizing the total production costs. This article aims to explore the possibility for optimizing the production of pharmaceutical products through the construction of a mathematical model that can be viewed in two ways – as a single-product model and a multi-product model. As an optimality criterion it is set the minimization of the cost per unit of production for a given planning period. The author proposes an analytical method for solving the nonlinear optimization problem. An optimal production plan of Tylosin tartrate is found using the single-product model.

  9. Algal and microbial exopolysaccharides: new insights as biosurfactants and bioemulsifiers.

    Science.gov (United States)

    Paniagua-Michel, José de Jesús; Olmos-Soto, Jorge; Morales-Guerrero, Eduardo Roberto

    2014-01-01

    Currently, efforts are being made to utilize more natural biological systems as alternatives as a way to replace fossil forms of carbon. There is a growing concern at global level to have nontoxic, nonhazardous surface-active agents; contrary to synthetic surfactants, their biological counterparts or biosurfactants play a primary function, facilitating microbial presence in environments dominated by hydrophilic-hydrophobic interfaces. Algal and microbial biosurfactants/bioemulsifiers from marine and deep-sea environments are attracting major interest due to their structural and functional diversity as molecules actives of surface and an alternative biomass to replace fossil forms of carbon. Algal and microbial surfactants are lipid in nature and classified as glycolipids, phospholipids, lipopeptides, natural lipids, fatty acids, and lipopolysaccharides. These metabolic bioactive products are applicable in a number of industries and processes, viz., food processing, pharmacology, and bioremediation of oil-polluted environments. This chapter presents an update of the progress and potentialities of the principal producers of exopolysaccharide (EPS)-type biosurfactants and bioemulsifiers, viz., macro- and microalgae (cyanobacteria and diatoms) and bacteria from marine and extreme environments. Particular interest is centered into new sources and applications, viz., marine and deep-sea environments and promissory uses of these EPSs as biosurfactants/emulsifiers and other polymeric roles. The enormous benefits of these molecules encourage their discovery, exploitation, and development of new microbial EPSs that could possess novel industrial importance and corresponding innovations. © 2014 Elsevier Inc. All rights reserved.

  10. Characterization of a novel biosurfactant produced by Staphylococcus sp. strain 1E with potential application on hydrocarbon bioremediation.

    Science.gov (United States)

    Eddouaouda, Kamel; Mnif, Sami; Badis, Abdelmalek; Younes, Sonia Ben; Cherif, Slim; Ferhat, Samira; Mhiri, Najla; Chamkha, Mohamed; Sayadi, Sami

    2012-08-01

    A biosurfactant-producing bacterium (Staphylococcus sp. strain 1E) was isolated from an Algerian crude oil contaminated soil. Biosurfactant production was tested with different carbon sources using the surface tension measurement and the oil displacement test. Olive oil produced the highest reduction in surface tension (25.9 dynes cm(-1)). Crude oil presented the best substrate for 1E biosurfactant emulsification activity. The biosurfactant produced by strain 1E reduced the growth medium surface tension below 30 dynes cm(-1). This reduction was also obtained in cell-free filtrates. Biosurfactant produced by strain 1E showed stability in a wide range of pH (from 2 to 12), temperature (from 4 to 55 °C) and salinity (from 0 to 300 g l(-1)) variations. The biosurfactant produced by strain 1E belonged to lipopeptide group and also constituted an antibacterial activity againt the pathogenic bacteria such as Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis. Phenanthrene solubility in water was enhanced by biosurfactant addition. Our results suggest that the 1E biosurfactant has interesting properties for its application in bioremediation of hydrocarbons contaminated sites. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  11. Alternatives for biosurfactants and bacteriocins extraction from Lactococcus lactis cultures produced under different pH conditions.

    Science.gov (United States)

    Rodríguez, N; Salgado, J M; Cortés, S; Domínguez, J M

    2010-08-01

    Study of the potential of Lactococcus lactis CECT-4434 as a biosurfactants and nisin (the only bacteriocin allowed to be used in the food industry) producer for industrial applications, exploiting the possibility of recovering separately both metabolites, taking into account that L. lactis is an interesting micro-organism with several applications in the food industry because it is recognized as GRAS. The results showed the ability of this strain to produce cell-bound biosurfactants, under controlled pH, and cell-bound biosurfactants and bacteriocins, when pH was not controlled. Three extraction procedures were designed to separately recover these substances. The strain L. lactis CECT-4434 showed to be a cell-bound biosurfactants and bacterocins producer when fermentations were carried out under uncontrolled pH. Both products can be recovered separately. Development of a convenient tool for the extraction of cell-bound biosurfactants and bacteriocins from the fermentation broth.

  12. STATEMENT OF THE OPTIMIZATION PROBLEM OF CARBON PRODUCTS PRODUCTION

    Directory of Open Access Journals (Sweden)

    O. A. Zhuchenko

    2016-08-01

    Full Text Available The paper formulated optimization problem formulation production of carbon products. The analysis of technical and economic parameters that can be used to optimize the production of carbonaceous products had been done by the author. To evaluate the efficiency of the energy-intensive production uses several technical and economic indicators. In particular, the specific cost, productivity, income and profitability of production. Based on a detailed analysis had been formulated optimality criterion that takes into account the technological components of profitability. The components in detail the criteria and the proposed method of calculating non-trivial, one of them - the production cost of each product. When solving the optimization problem of technological modes of production into account constraints on the variables are optimized. Thus, restrictions may be expressed on the number of each product produced. Have been formulated the method of calculating the cost per unit of product. Attention is paid to the quality indices of finished products as an additional constraint in the optimization problem. As a result have been formulated the general problem of optimizing the production of carbon products, which includes the optimality criterion and restrictions.

  13. Halomonas sp. BS4, A biosurfactant producing halophilic bacterium isolated from solar salt works in India and their biomedical importance

    OpenAIRE

    Donio, Mariathason Birdilla Selva; Ronica, Fernando Arul; Viji, Vijayaragavan Thanga; Velmurugan, Subramanian; Jenifer, John Selesteen Charles Adlin; Michaelbabu, Mariavincent; Dhar, Prasenjit; Citarasu, Thavasimuthu

    2013-01-01

    Halophilic bacteria were isolated from Thamaraikulam solar salt works in India. After routine biosurfactant screening by various methods, the biosurfactant producing bacteria, Halomonas sp BS4 was confirmed by 16?S rRNA sequencing. The growth optimization of Halomonas sp BS4 revealed their optimum growth at 8% NaCl and 6-8?pH in the growth medium. Further the partially purified biosurfactants were characterized by TLC, FTIR and GC-MS analysis. GC-MS results revealed that, the partial purified...

  14. Microbiological Production of Surfactant from Agricultural Residuals for IOR Application

    Energy Technology Data Exchange (ETDEWEB)

    Bala, Greg Alan; Bruhn, Debby Fox; Fox, Sandra Lynn; Noah, Karl Scott; Thompson, David Neal

    2002-04-01

    Utilization of surfactants for improved oil recovery (IOR) is an accepted technique with high potential. However, technology application is frequently limited by cost. Biosurfactants (surface-active molecules produced by microorganisms) are not widely utilized in the petroleum industry due to high production costs associated with use of expensive substrates and inefficient product recovery methods. The economics of biosurfactant production could be significantly impacted through use of media optimization and application of inexpensive carbon substrates such as agricultural process residuals. Utilization of biosurfactants produced from agricultural residuals may 1) result in an economic advantage for surfactant production and technology application, and 2) convert a substantial agricultural waste stream to a value-added product for IOR. A biosurfactant with high potential for use is surfactin, a lipopeptide biosurfactant, produced by Bacillus subtilis. Reported here is the production and potential IOR utilization of surfactin produced by Bacillus subtilis (American Type Culture Collection (ATCC) 21332) from starch-based media. Production of surfactants from microbiological growth media based on simple sugars, chemically pure starch medium, simulated liquid and solid potato-process effluent media, a commercially prepared potato starch in mineral salts, and process effluent from a potato processor is discussed. Additionally, the effect of chemical and physical pretreatments on starchy feedstocks is discussed.

  15. OPTIMAL PRICING OF A PERSONALIZED PRODUCT

    Institute of Scientific and Technical Information of China (English)

    Suresh P.SETHI

    2008-01-01

    This paper deals with optimal pricing of a personalized product such as a personal portrait or photo.A new model of the pricing structure inspired by two real-life cases is introduced to the literature and solved to obtain optimal photo sitting fees and the final product price.A sensitivity analysis with respect to the problem parameters is performed.

  16. Characterization of Biosurfactant Produced during Degradation of Hydrocarbons Using Crude Oil As Sole Source of Carbon

    Science.gov (United States)

    Patowary, Kaustuvmani; Patowary, Rupshikha; Kalita, Mohan C.; Deka, Suresh

    2017-01-01

    Production and spillage of petroleum hydrocarbons which is the most versatile energy resource causes disastrous environmental pollution. Elevated oil degrading performance from microorganisms is demanded for successful microbial remediation of those toxic pollutants. The employment of biosurfactant-producing and hydrocarbon-utilizing microbes enhances the effectiveness of bioremediation as biosurfactant plays a key role by making hydrocarbons bio-available for degradation. The present study aimed the isolation of a potent biosurfactant producing indigenous bacteria which can be employed for crude oil remediation, along with the characterization of the biosurfactant produced during crude oil biodegradation. A potent bacterial strain Pseudomonas aeruginosa PG1 (identified by 16s rDNA sequencing) was isolated from hydrocarbon contaminated soil that could efficiently produce biosurfactant by utilizing crude oil components as the carbon source, thereby leading to the enhanced degradation of the petroleum hydrocarbons. Strain PG1 could degrade 81.8% of total petroleum hydrocarbons (TPH) after 5 weeks of culture when grown in mineral salt media (MSM) supplemented with 2% (v/v) crude oil as the sole carbon source. GCMS analysis of the treated crude oil samples revealed that P. aeruginosa PG1 could potentially degrade various hydrocarbon contents including various PAHs present in the crude oil. Biosurfactant produced by strain PG1 in the course of crude oil degradation, promotes the reduction of surface tension (ST) of the culture medium from 51.8 to 29.6 mN m−1, with the critical micelle concentration (CMC) of 56 mg L−1. FTIR, LC-MS, and SEM-EDS studies revealed that the biosurfactant is a rhamnolipid comprising of both mono and di rhamnolipid congeners. The biosurfactant did not exhibit any cytotoxic effect to mouse L292 fibroblastic cell line, however, strong antibiotic activity against some pathogenic bacteria and fungus was observed. PMID:28275373

  17. Characterization of Biosurfactant Produced during Degradation of Hydrocarbons Using Crude Oil As Sole Source of Carbon.

    Science.gov (United States)

    Patowary, Kaustuvmani; Patowary, Rupshikha; Kalita, Mohan C; Deka, Suresh

    2017-01-01

    Production and spillage of petroleum hydrocarbons which is the most versatile energy resource causes disastrous environmental pollution. Elevated oil degrading performance from microorganisms is demanded for successful microbial remediation of those toxic pollutants. The employment of biosurfactant-producing and hydrocarbon-utilizing microbes enhances the effectiveness of bioremediation as biosurfactant plays a key role by making hydrocarbons bio-available for degradation. The present study aimed the isolation of a potent biosurfactant producing indigenous bacteria which can be employed for crude oil remediation, along with the characterization of the biosurfactant produced during crude oil biodegradation. A potent bacterial strain Pseudomonas aeruginosa PG1 (identified by 16s rDNA sequencing) was isolated from hydrocarbon contaminated soil that could efficiently produce biosurfactant by utilizing crude oil components as the carbon source, thereby leading to the enhanced degradation of the petroleum hydrocarbons. Strain PG1 could degrade 81.8% of total petroleum hydrocarbons (TPH) after 5 weeks of culture when grown in mineral salt media (MSM) supplemented with 2% (v/v) crude oil as the sole carbon source. GCMS analysis of the treated crude oil samples revealed that P. aeruginosa PG1 could potentially degrade various hydrocarbon contents including various PAHs present in the crude oil. Biosurfactant produced by strain PG1 in the course of crude oil degradation, promotes the reduction of surface tension (ST) of the culture medium from 51.8 to 29.6 mN m -1 , with the critical micelle concentration (CMC) of 56 mg L -1 . FTIR, LC-MS, and SEM-EDS studies revealed that the biosurfactant is a rhamnolipid comprising of both mono and di rhamnolipid congeners. The biosurfactant did not exhibit any cytotoxic effect to mouse L292 fibroblastic cell line, however, strong antibiotic activity against some pathogenic bacteria and fungus was observed.

  18. Biosurfactants, bioemulsifiers and exopolysaccharides from marine microorganisms.

    Science.gov (United States)

    Satpute, Surekha K; Banat, Ibrahim M; Dhakephalkar, Prashant K; Banpurkar, Arun G; Chopade, Balu A

    2010-01-01

    Marine biosphere offers wealthy flora and fauna, which represents a vast natural resource of imperative functional commercial grade products. Among the various bioactive compounds, biosurfactant (BS)/bioemulsifiers (BE) are attracting major interest and attention due to their structural and functional diversity. The versatile properties of surface active molecules find numerous applications in various industries. Marine microorganisms such as Acinetobacter, Arthrobacter, Pseudomonas, Halomonas, Myroides, Corynebacteria, Bacillus, Alteromonas sp. have been studied for production of BS/BE and exopolysaccharides (EPS). Due to the enormity of marine biosphere, most of the marine microbial world remains unexplored. The discovery of potent BS/BE producing marine microorganism would enhance the use of environmental biodegradable surface active molecule and hopefully reduce total dependence or number of new application oriented towards the chemical synthetic surfactant industry. Our present review gives comprehensive information on BS/BE which has been reported to be produced by marine microorganisms and their possible potential future applications.

  19. Yeasts and bacterial biosurfactants as demulsifiers for petroleum derivative in seawater emulsions.

    Science.gov (United States)

    Rocha E Silva, Fernanda Cristina P; Roque, Bruno Augusto C; Rocha E Silva, Nathalia Maria P; Rufino, Raquel D; Luna, Juliana M; Santos, Valdemir A; Banat, Ibrahim M; Sarubbo, Leonie A

    2017-11-15

    Oil sludge or waste generated in transport, storage or refining forms highly stable mixtures due to the presence and additives with surfactant properties and water forming complex emulsions. Thus, demulsification is necessary to separate this residual oil from the aqueous phase for oil processing and water treatment/disposal. Most used chemical demulsifiers, although effective, are environmental contaminants and do not meet the desired levels of biodegradation. We investigated the application of microbial biosurfactants as potential natural demulsifiers of petroleum derivatives in water emulsions. Biosurfactants crude extracts, produced by yeasts (Candida guilliermondii, Candida lipolytica and Candida sphaerica) and bacteria (Pseudomonas aeruginosa, Pseudomonas cepacia and Bacillus sp.) grown in industrial residues, were tested for demulsification capacity in their crude and pure forms. The best results obtained were for bacterial biosurfactants, which were able to recover about 65% of the seawater emulsified with motor oil compared to 35-40% only for yeasts products. Biosurfactants were also tested with oil-in-water (O/W) and water-in-oil (W/O) kerosene model emulsions. No relationship between interfacial tension, cell hydrophobicity and demulsification ratios was observed with all the biosurfactants tested. Microscopic illustrations of the emulsions in the presence of the biosurfactants showed the aspects of the emulsion and demulsification process. The results obtained demonstrate the potential of these agents as demulsifiers in marine environments.

  20. Isolation and characterization of halophilic Bacillus sp. BS3 able to produce pharmacologically important biosurfactants.

    Science.gov (United States)

    Donio, M B S; Ronica, S F A; Viji, V Thanga; Velmurugan, S; Jenifer, J Adlin; Michaelbabu, M; Citarasu, T

    2013-11-01

    To characterize the pharmacological importance of biosurfactants isolated from halophilic Bacillus sp BS3. Halophilic Bacillus sp. BS3 was isolated from solar salt works, identified by 16S rRNA sequencing and was used for screening their biosurfactant production. Characters of the biosurfactant and their anticancer activity were analyzed and performed in mammary epithelial carcinoma cell at different concentrations. The biosurfactant were characterized by TLC, FTIR and GC-MS analysis and identified as lipopeptide type. GC-MS analysis revealed that, the biosurfactant had various compounds including 13-Docosenamide, (Z); Mannosamine, 9- and N,N,N',N'-tetramethyl. Surprisingly the antiviral activity was found against shrimp white spot syndrome virus (WSSV) by suppressing the viral replication and significantly raised shrimp survival (Pbiosurfactants, among the various concentrations of biosurfactants such as 0.000 25, 0.002 5, 0.025, 0.25 and 2.5 μg, the 0.25 μg concentration suppressed the cells significantly (P<0.05) to 24.8%. Based on the findings, the present study concluded that, there is a possibility to develop eco-friendly antimicrobial and anticancer drugs from the extremophilic origin. Copyright © 2013 Hainan Medical College. Published by Elsevier B.V. All rights reserved.

  1. Multivariate optimization of production systems

    International Nuclear Information System (INIS)

    Carroll, J.A.; Horne, R.N.

    1992-01-01

    This paper reports that mathematically, optimization involves finding the extreme values of a function. Given a function of several variables, Z = ∫(rvec x 1 , rvec x 2 ,rvec x 3 ,→x n ), an optimization scheme will find the combination of these variables that produces an extreme value in the function, whether it is a minimum or a maximum value. Many examples of optimization exist. For instance, if a function gives and investor's expected return on the basis of different investments, numerical optimization of the function will determine the mix of investments that will yield the maximum expected return. This is the basis of modern portfolio theory. If a function gives the difference between a set of data and a model of the data, numerical optimization of the function will produce the best fit of the model to the data. This is the basis for nonlinear parameter estimation. Similar examples can be given for network analysis, queuing theory, decision analysis, etc

  2. Scale up and application of biosurfactant from Bacillus subtilis in Enhanced Oil recovery.

    Science.gov (United States)

    Amani, Hossein; Mehrnia, Mohammad Reza; Sarrafzadeh, Mohammad Hossein; Haghighi, Manouchehr; Soudi, Mohammad Reza

    2010-09-01

    There is a lack of fundamental knowledge about the scale up of biosurfactant production. In order to develop suitable technology of commercialization, carrying out tests in shake flasks and bioreactors was essential. A reactor with integrated foam collector was designed for biosurfactant production using Bacillus subtilis isolated from agricultural soil. The yield of biosurfactant on biomass (Y(p/x)), biosurfactant on sucrose (Y(p/s)), and the volumetric production rate (Y) for shake flask were obtained about 0.45 g g(-1), 0.18 g g(-1), and 0.03 g l(-1) h(-1), respectively. The best condition for bioreactor was 300 rpm and 1.5 vvm, giving Y(x/s), Y(p/x), Y(p/s), and Y of 0.42 g g(-1), 0.595 g g(-1), 0.25 g g(-1), and 0.057 g l(-1) h(-1), respectively. The biosurfactant maximum production, 2.5 g l(-1), was reached in 44 h of growth, which was 28% better than the shake flask. The obtained volumetric oxygen transfer coefficient (K(L)a) values at optimum conditions in the shake flask and the bioreactor were found to be around 0.01 and 0.0117 s(-1), respectively. Comparison of K(L)a values at optimum conditions shows that biosurfactant production scaling up from shake flask to bioreactor can be done with K(L) a as scale up criterion very accurately. Nearly 8% of original oil in place was recovered using this biosurfactant after water flooding in the sand pack.

  3. Optimal Control Inventory Stochastic With Production Deteriorating

    Science.gov (United States)

    Affandi, Pardi

    2018-01-01

    In this paper, we are using optimal control approach to determine the optimal rate in production. Most of the inventory production models deal with a single item. First build the mathematical models inventory stochastic, in this model we also assume that the items are in the same store. The mathematical model of the problem inventory can be deterministic and stochastic models. In this research will be discussed how to model the stochastic as well as how to solve the inventory model using optimal control techniques. The main tool in the study problems for the necessary optimality conditions in the form of the Pontryagin maximum principle involves the Hamilton function. So we can have the optimal production rate in a production inventory system where items are subject deterioration.

  4. Chemical characterization of carbohydrate-based biosurfactants

    Science.gov (United States)

    High-yield, glycolipid-based biosurfactants are of increasing interest for use in environmentally benign cleaning or emulsifying agents. We have developed a MALDI-TOF/MS screen for the rapid analysis of several types of biosurfactants, including various acylated rhamnolipids in Pseudomonas extracts...

  5. Potential biosurfactant producing endophytic and epiphytic fungi ...

    African Journals Online (AJOL)

    Potential biosurfactant producing endophytic and epiphytic fungi, isolated from macrophytes in the Negro River in Manaus, Amazonas, Brazil. ... Solms and Cyperus ligularis L., macrophytes collected from oil-contaminated waters, were studied to assess their potential for producing biosurfactants; the most promising ones ...

  6. Biosurfactant Mediated Biosynthesis of Selected Metallic Nanoparticles

    Science.gov (United States)

    Płaza, Grażyna A.; Chojniak, Joanna; Banat, Ibrahim M.

    2014-01-01

    Developing a reliable experimental protocol for the synthesis of nanomaterials is one of the challenging topics in current nanotechnology particularly in the context of the recent drive to promote green technologies in their synthesis. The increasing need to develop clean, nontoxic and environmentally safe production processes for nanoparticles to reduce environmental impact, minimize waste and increase energy efficiency has become essential in this field. Consequently, recent studies on the use of microorganisms in the synthesis of selected nanoparticles are gaining increased interest as they represent an exciting area of research with considerable development potential. Microorganisms are known to be capable of synthesizing inorganic molecules that are deposited either intra- or extracellularly. This review presents a brief overview of current research on the use of biosurfactants in the biosynthesis of selected metallic nanoparticles and their potential importance. PMID:25110864

  7. Biosurfactant Mediated Biosynthesis of Selected Metallic Nanoparticles

    Directory of Open Access Journals (Sweden)

    Grażyna A. Płaza

    2014-08-01

    Full Text Available Developing a reliable experimental protocol for the synthesis of nanomaterials is one of the challenging topics in current nanotechnology particularly in the context of the recent drive to promote green technologies in their synthesis. The increasing need to develop clean, nontoxic and environmentally safe production processes for nanoparticles to reduce environmental impact, minimize waste and increase energy efficiency has become essential in this field. Consequently, recent studies on the use of microorganisms in the synthesis of selected nanoparticles are gaining increased interest as they represent an exciting area of research with considerable development potential. Microorganisms are known to be capable of synthesizing inorganic molecules that are deposited either intra- or extracellularly. This review presents a brief overview of current research on the use of biosurfactants in the biosynthesis of selected metallic nanoparticles and their potential importance.

  8. The optimal sampling of outsourcing product

    International Nuclear Information System (INIS)

    Yang Chao; Pei Jiacheng

    2014-01-01

    In order to improve quality and cost, the sampling c = 0 has been introduced to the inspection of outsourcing product. According to the current quality level (p = 0.4%), we confirmed the optimal sampling that is: Ac = 0; if N ≤ 3000, n = 55; 3001 ≤ N ≤ 10000, n = 86; N ≥ 10001, n = 108. Through analyzing the OC curve, we came to the conclusion that when N ≤ 3000, the protective ability of optimal sampling for product quality is stronger than current sampling. Corresponding to the same 'consumer risk', the product quality of optimal sampling is superior to current sampling. (authors)

  9. Management of soybean oil refinery wastes through recycling them for producing biosurfactant using Pseudomonas aeruginosa MR01.

    Science.gov (United States)

    Partovi, Maryam; Lotfabad, Tayebe Bagheri; Roostaazad, Reza; Bahmaei, Manochehr; Tayyebi, Shokoufe

    2013-06-01

    Biosurfactant production through a fermentation process involving the biodegradation of soybean oil refining wastes was studied. Pseudomonas aeruginosa MR01 was able to produce extracellular biosurfactant when it was cultured in three soybean oil refinement wastes; acid oil, deodorizer distillate and soapstock, at different carbon to nitrogen ratios. Subsequent fermentation kinetics in the three types of waste culture were also investigated and compared with kinetic behavior in soybean oil medium. Biodegradation of wastes, biosurfactant production, biomass growth, nitrate consumption and the number of colony forming units were detected in four proposed media, at specified time intervals. Unexpectedly, wastes could stimulate the biodegradation activity of MR01 bacterial cells and thus biosurfactant synthesis beyond that of the refined soybean oil. This is evident from higher yields of biodegradation and production, as revealed in the waste cultures (Ydeg|(Soybean oil) = 53.9 % YP/S|(Soybean oil) = 0.31 g g(-1), respectively). Although production yields were approximately the same in the three waste cultures (YP/S|(wastes) =/~ 0.5 g g(-1)), microbial activity resulted in higher yields of biodegradation (96.5 ± 1.13 %), maximum specific growth rate (μ max = 0.26 ± 0.02 h(-1)), and biosurfactant purity (89.6 %) with a productivity of 14.55 ± 1.10 g l(-1), during the bioconversion of soapstock into biosurfactant. Consequently, applying soybean oil soapstock as a substrate for the production of biosurfactant with commercial value has the potential to provide a combination of economical production with environmental protection through the biosynthesis of an environmentally friendly (green) compound and reduction of waste load entering the environment. Moreover, this work inferred spectrophotometry as an easy method to detect rhamnolipids in the biosurfactant products.

  10. Biosurfactant production for application in soil remediation contaminated by oil: study of the virulence factors; Producao de biosurfactantes para aplicacao em remediacao de solos contaminados por petroleo: estudo dos fatores de virulencia

    Energy Technology Data Exchange (ETDEWEB)

    Fernandes, Ana Carolina Loureiro Brito; Pereira, Marcos Dias; Freire, Denise Maria Guimaraes [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Inst. de Quimica; Kronemberger, Frederico de Araujo [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil). Coordenacao dos Programas de Pos-Graduacao de Engenharia (COPPE)

    2008-07-01

    Biosurfactants are amphipatic molecules produced by microorganisms. The rhamnolipid (RML) is a group of those molecules, most produced by strains of Pseudomonas. In this present work, the effect of superficial aeration on the production of rhamnolipids and virulence factors, from proteins produced by Pseudomonas aeruginosa PA1, isolated from oil extraction wells, was evaluated. Cell growth, glycerol and nitrate consumption, rhamnolipid production, nonspecific proteases, total proteins and elastases where investigated on different flask volume/medium volume relations (Vf/Vm): 1:0,15, 1:0,30, 1:0,50 and 1:0,70. It was observed that better oxygenation leaded to better results on the rhamnolipid production. Two peaks of elastase activity were noted, showing possible presence of two types of elastases that could be dislocated on fermentation time, due to the oxygenation degree. The major production of rhamnolipid was found on the 1:0,50 condition and different non-specific proteases standards were found due to the oxygenation degrees. (author)

  11. Product portfolio optimization based on substitution

    DEFF Research Database (Denmark)

    Myrodia, Anna; Moseley, A.; Hvam, Lars

    2017-01-01

    The development of production capabilities has led to proliferation of the product variety offered to the customer. Yet this fact does not directly imply increase of manufacturers' profitability, nor customers' satisfaction. Consequently, recent research focuses on portfolio optimization through...... substitution and standardization techniques. However when re-defining the strategic market decisions are characterized by uncertainty due to several parameters. In this study, by using a GAMS optimization model we present a method for supporting strategic decisions on substitution, by quantifying the impact...

  12. Optimal Pricing Strategy for New Products

    OpenAIRE

    Trichy V. Krishnan; Frank M. Bass; Dipak C. Jain

    1999-01-01

    Robinson and Lakhani (1975) initiated a long research stream in marketing when they used the Bass model (1969) to develop optimal pricing path for a new product. A careful analysis of the extant literature reveals that the research predominantly suggests that the optimal price path should be largely based on the sales growth pattern. However, in the real world we rarely find new products that have such pricing pattern. We observe either a monotonically declining pricing pattern or an increase...

  13. Production Optimization of Oil Reservoirs

    DEFF Research Database (Denmark)

    Völcker, Carsten

    with emphasis on optimal control of water ooding with the use of smartwell technology. We have implemented immiscible ow of water and oil in isothermal reservoirs with isotropic heterogenous permeability elds. We use the method of lines for solution of the partial differential equation (PDE) system that governs...... the uid ow. We discretize the the two-phase ow model spatially using the nite volume method (FVM), and we use the two point ux approximation (TPFA) and the single-point upstream (SPU) scheme for computing the uxes. We propose a new formulation of the differential equation system that arise...... as a consequence of the spatial discretization of the two-phase ow model. Upon discretization in time, the proposed equation system ensures the mass conserving property of the two-phase ow model. For the solution of the spatially discretized two-phase ow model, we develop mass conserving explicit singly diagonally...

  14. Isolation and characterization of a biosurfactant producing strain, Brevibacilis brevis HOB1.

    Science.gov (United States)

    Haddad, Namir I A; Wang, Ji; Mu, Bozhong

    2008-12-01

    Biosurfactant-producing bacteria were isolated from the production water of an oil field. Isolates were screened for biosurfactant production using surface tension test. The highest reduction of surface tension was achieved with a bacterial strain which was identified by 16S rRNA gene sequencing as Brevibacilis brevis HOB1. It has been investigated using different carbon and nitrogen sources. It showed that the strain was able to grow and reduce the surface tension of the broth to 29 mN/m on commercial sugar and maltose, and to 32 mN/m on glucose after 72 h of growth. The maximum amount of biosurfactant was obtained when nitrate ions were supplied as nitrogen source. Biosurfactant produced by Brevibacilis brevis HOB1 was confirmed as a lipopeptide class of biosurfactant using TLC test and mass spectra. Lipopeptide isoforms were isolated from cell-free supernatants by acid-precipitation followed by one step of chromatographic separation on solid-phase ODS C18 column. The separation was confirmed by HPLC and ESI Q-TOF MS spectroscopy. Comparing the mass data obtained and the mass numbers reported for the lipopeptide complexes from other strains, it can be concluded that the major lipopeptide product of Brevibacilis brevis HOB1 is the surfactin isoform. This lipopeptide showed strong antibacterial and antifungal activity. It is a candidate for the biocontrol of pathogens in agriculture and other industries.

  15. OPTIMIZATION METHODS IN TRANSPORTATION OF FOREST PRODUCTS

    Directory of Open Access Journals (Sweden)

    Selçuk Gümüş

    2008-04-01

    Full Text Available Turkey has total of 21.2 million ha (27 % forest land. In this area, average 9 million m3 of logs and 5 million stere of fuel wood have been annually produced by the government forest enterprises. The total annual production is approximately 13million m3 Considering the fact that the costs of transporting forest products was about . 160 million TL in the year of 2006, the importance of optimizing the total costs in transportation can be better understood. Today, there is not common optimization method used at whole transportation problems. However, the decision makers select the most appropriate methods according to their aims.Comprehending of features and capacity of optimization methods is important for selecting of the most appropriate method. The evaluation of optimization methods that can be used at forest products transportation is aimed in this study.

  16. Bacillus amyloliquefaciens TSBSO 3.8, a biosurfactant-producing strain with biotechnological potential for microbial enhanced oil recovery.

    Science.gov (United States)

    Alvarez, Vanessa Marques; Jurelevicius, Diogo; Marques, Joana Montezano; de Souza, Pamella Macedo; de Araújo, Livia Vieira; Barros, Thalita Gonçalves; de Souza, Rodrigo Octavio Mendonça Alves; Freire, Denise Maria Guimarães; Seldin, Lucy

    2015-12-01

    A screening for biosurfactant-producing bacteria was conducted with 217 strains that were isolated from environmental samples contaminated with crude oil and/or petroleum derivatives. Although 19 promising biosurfactant producers were detected, strain TSBSO 3.8, which was identified by molecular methods as Bacillus amyloliquefaciens, drew attention for its production of a high-activity compound that presented an emulsification activity of 63% and considerably decreased surface (28.5 mN/m) and interfacial (11.4 mN/m) tensions in Trypticase Soy Broth culture medium. TSBSO 3.8 growth and biosurfactant production were tested under different physical and chemical conditions to evaluate its biotechnological potential. Biosurfactant production occurred between 0.5% and 7% NaCl, at pH values varying from 6 to 9 and temperatures ranging from 28 to 50 °C. Moreover, biosurfactant properties remained the same after autoclaving at 121 °C for 15 min. The biosurfactant was also successful in a test to simulate microbial enhanced oil recovery (MEOR). Mass spectrometry analysis showed that the surface active compound was a surfactin, known as a powerful biosurfactant that is commonly produced by Bacillus species. The production of a high-efficiency biosurfactant, under some physical and chemical conditions that resemble those experienced in an oil production reservoir, such as high salinities and temperatures, makes TSBSO 3.8 an excellent candidate and creates good expectations for its application in MEOR. Copyright © 2015 Elsevier B.V. All rights reserved.

  17. Biosurfactant produced from Actinomycetes nocardiopsis A17: Characterization and its biological evaluation.

    Science.gov (United States)

    Chakraborty, Samrat; Ghosh, Mandakini; Chakraborti, Srijita; Jana, Sougata; Sen, Kalyan Kumar; Kokare, Chandrakant; Zhang, Lixin

    2015-08-01

    This investigation aims to isolate an Actinomycetes strain producing a biosurfactant from the unexplored region of industrial and coal mine areas. Actinomycetes are selected for this study as their novel chemistry was not exhausted and they have tremendous potential to produce bioactive secondary metabolites. The biosurfactant was characterized and further needed to be utilized for pharmaceutical dosage form. Isolation, purification, screening, and characterization of the Actinomycetes A17 were done followed by its fermentation in optimized conditions. The cell-free supernatant was used for the extraction of the biosurfactant and precipitated by cold acetone. The dried precipitate was purified by TLC and the emulsification index, surface tension and CMC were determined. The isolated strain with preferred results was identified as Actinomycetes nocardiopsis A17 with high foam-forming properties. It gives lipase, amylase, gelatinase, and protease activity. The emulsification index was found to be 93±0.8 with surface tension 66.67 dyne/cm at the lowest concentration and cmc 0.6 μg/ml. These biosurfactants were characterized by Fourier transform infra red (FT-IR) spectroscopy and liquid chromatography-mass spectrometry (LC-MS). Therefore, it can be concluded that the biosurfactant produced by Actinomycetes nocardiopsis sp. strain A17 was found to have satisfactory results with high surface activity and emulsion-forming ability. Copyright © 2015 Elsevier B.V. All rights reserved.

  18. Applications of biosurfactants in the petroleum industry and the remediation of oil spills.

    Science.gov (United States)

    de Cássia F S Silva, Rita; Almeida, Darne G; Rufino, Raquel D; Luna, Juliana M; Santos, Valdemir A; Sarubbo, Leonie Asfora

    2014-07-15

    Petroleum hydrocarbons are important energy resources. However, petroleum is also a major pollutant of the environment. Contamination by oil and oil products has caused serious harm, and increasing attention has been paid to the development and implementation of innovative technologies for the removal of these contaminants. Biosurfactants have been extensively used in the remediation of water and soil, as well as in the main stages of the oil production chain, such as extraction, transportation, and storage. This diversity of applications is mainly due to advantages such as biodegradability, low toxicity and better functionality under extreme conditions in comparison to synthetic counterparts. Moreover, biosurfactants can be obtained with the use of agro-industrial waste as substrate, which helps reduce overall production costs. The present review describes the potential applications of biosurfactants in the oil industry and the remediation of environmental pollution caused by oil spills.

  19. Applications of Biosurfactants in the Petroleum Industry and the Remediation of Oil Spills

    Directory of Open Access Journals (Sweden)

    Rita de Cássia F. S. Silva

    2014-07-01

    Full Text Available Petroleum hydrocarbons are important energy resources. However, petroleum is also a major pollutant of the environment. Contamination by oil and oil products has caused serious harm, and increasing attention has been paid to the development and implementation of innovative technologies for the removal of these contaminants. Biosurfactants have been extensively used in the remediation of water and soil, as well as in the main stages of the oil production chain, such as extraction, transportation, and storage. This diversity of applications is mainly due to advantages such as biodegradability, low toxicity and better functionality under extreme conditions in comparison to synthetic counterparts. Moreover, biosurfactants can be obtained with the use of agro-industrial waste as substrate, which helps reduce overall production costs. The present review describes the potential applications of biosurfactants in the oil industry and the remediation of environmental pollution caused by oil spills.

  20. Lipopeptide biosurfactant from Bacillus thuringiensis pak2310: A potential antagonist against Fusarium oxysporum.

    Science.gov (United States)

    Deepak, R; Jayapradha, R

    2015-03-01

    The aims of the study were to evaluate the effects of a biosurfactant obtained from a novel Bacillus thuringiensis on Fusarium oxysporum to determine the morphological changes in the structure of the fungi and its biofilm in the presence of the biosurfactant and to evaluate the toxicity of the biosurfactant on HEp-2 human epithelial cell lines. The strain was screened and isolated from petroleum contaminated soil based on the E24 emulsification index. The biosurfactant was produced on glycerol, extracted using chloroform:methanol system and purified using HPLC. The purified fraction showing both surface activity (emulsification and oil-spread activity) and anti-fusarial activity (agar well diffusion method) was studied using FT-IR and MALDI-TOF MS, respectively. The minimum inhibitory concentration (MIC) and the biofilm inhibitory concentration (BIC) were determined using dilution method. The effect of biosurfactant on the morphology of Fusarium oxysporum was monitored using light microscopy and confocal laser scanning microscopy (for biofilm). The purified surfactant showed the presence of functional groups like that of surfactin in the FT-IR spectra and MALDI-TOF MS estimated the molecular weight as 700Da. The MIC and BIC were estimated to be 0.05 and 0.5mg/mL, respectively. The molecule was also non-toxic to HEp-2 cell lines at 10× MIC. A non-toxic and effective anti-Fusarium biosurfactant, that is both safe for human use and to the environment, has been characterized. The growth and metabolite production using glycerol (major byproduct of biodiesel and soap industries) also adds up to the efficiency and ecofriendly nature of this biosurfactant. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  1. Evaluation of dermal wound healing and in vitro antioxidant efficiency of Bacillus subtilis SPB1 biosurfactant.

    Science.gov (United States)

    Zouari, Raida; Moalla-Rekik, Dorsaf; Sahnoun, Zouheir; Rebai, Tarek; Ellouze-Chaabouni, Semia; Ghribi-Aydi, Dhouha

    2016-12-01

    Lipopeptide microbial surfactants are endowed with unique surface properties as well as antimicrobial, anti-wrinkle, moisturizing and free radical scavenging activities. They were introduced safely in dermatological products, as long as they present low cytotoxicity against human cells. The present study was undertaken to evaluate the in vitro antioxidant activities and the wound healing potential of Bacillus subtilis SPB1 lipopeptide biosurfactant on excision wounds induced in experimental rats. The scavenging effect of Bacillus subtilis SPB1 biosurfactant on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical at 1mg/mL was 70.4% (IC 50 =0.55mg/mL). The biosurfactant produced by Bacillus subtilis SPB1 also showed good reducing power and significant effects in terms of the β-carotene test (IC 50 =2.26mg/mL) when compared to BHA as a reference standard. Moreover, an interesting ferrous ion chelating activity (80.32%) was found for SPB1 biosurfactant at 1mg/mL. Furthermore, the topical application of Bacillus subtilis SPB1 biosurfactant based gel on the wound site in a rat model every two days, increased significantly the percentage of wound closure over a period of 13days, when compared to the untreated and CICAFLORA™-treated groups. Wound healing effect of SPB1 biosurfactant based gel was confirmed by histological study. Biopsies treated with SPB1 lipopeptides showed wholly re-epithelialized wound with a perfect epidermal regeneration. The present study provides justification for the use of Bacillus subtilis SPB1 lipopeptide biosurfactant based gel for the treatment of normal and complicated wounds as well as skin diseases. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  2. Optimal mode of operation for biomass production

    NARCIS (Netherlands)

    Betlem, Ben H.L.; Roffel, Brian; Mulder, P.

    2002-01-01

    The rate of biomass production is optimised for a predefined feed exhaustion using the residue ratio as a degree of freedom. Three modes of operation are considered: continuous, repeated batch, and repeated fed-batch operation. By means of the Production Curve, the transition points of the optimal

  3. Biosurfactants in cosmetics and biopharmaceuticals.

    Science.gov (United States)

    Varvaresou, A; Iakovou, K

    2015-09-01

    Biosurfactants are surface-active biomolecules that are produced by various micro-organisms. They show unique properties i.e. lower toxicity, higher biodegradability and environmental compatibility compared to their chemical counterparts. Glycolipids and lipopeptides have prompted application in biotechnology and cosmetics due to their multi-functional profile i.e. detergency, emulsifying, foaming and skin hydrating properties. Additionally, some of them can be served as antimicrobials. In this study the current status of research and development on rhamnolipids, sophorolipids, mannosyloerythritol lipids, trehalipids, xylolipids and lipopeptides particularly their commercial application in cosmetics and biopharmaceuticals, is described. © 2015 The Society for Applied Microbiology.

  4. Optimization of Microbial Elastase Production

    International Nuclear Information System (INIS)

    Abd EI-Aziz, A.B.; Hassan, A.A.

    2010-01-01

    The extra cellular proteases (caseinase, gelatinase and elastase) and hemolytic activities of the tested microorganisms on agar plates were detected, using different substrates (gelatin, casein, hemoglobin and elastin).The proteolytic activities were detected only from Pseudomonas aeruginosa, Prevotella bivius, Bacillus subtilis and Micrococcus luteus. The production of elastase by Bacillus subtilis (has low hemolysins activity) at various temperatures (30 degree C - 37 degree C) and at exposure to different doses of gamma irradiation (0.25-1.0 kGy) was investigated in shake flask. The results indicated that the incubation temperature 37 degree C was the optimum for cell growth at earlier stage; while maximum elastase activity was obtained when the cells were cultivated at 30 degree C and irradiation dose level of 0.75 kGy. The effects of temperature, substrate content, elastase concentration, ph and different metals ions on elastolysis were investigated as well the elastase amino acids composition was detected by using amino acids analyzer

  5. Isolation of thermotolerant, halotolerant, facultative biosurfactant-producing bacteria.

    Science.gov (United States)

    Ghojavand, H; Vahabzadeh, F; Mehranian, M; Radmehr, M; Shahraki, Kh A; Zolfagharian, F; Emadi, M A; Roayaei, E

    2008-10-01

    Several facultative bacterial strains tolerant to high temperature and salinity were isolated from the oil reservoir brines of an Iranian oil field (Masjed-I Soleyman). Some of these isolates were able to grow up to 60 degrees C and at high concentration of NaCl (15% w/v). One of the isolates grew at 40 degrees C, while it was able to grow at 15% w/v NaCl. Tolerances to NaCl levels decreased as the growth temperatures were increased. Surfactant production ability was detected in some of these isolates. The use of biosurfactant is considered as an effective mechanism in microbial-enhanced oil recovery processes detected in some of these isolates. The surfactant producers were able to grow at high temperatures and salinities to about 55 degrees C and 10% w/v, respectively. These isolates exhibited morphological and physiological characteristics of the Bacillus genus. The partial sequencing of the 16S ribosomal deoxyribonucleic acid gene of the selected isolates was assigned them to Bacillus subtilis group. The biosurfactant produced by these isolates caused a substantial decrease in the surface tension of the culture media to 26.7 mN/m. By the use of thin-layer chromatography technique, the presence of the three compounds was detected in the tested biosurfactant. Infrared spectroscopy and (1)H nuclear magnetic resonance analysis were used, and the partial structural characterization of the biosurfactant mixture of the three compounds was found to be lipopeptidic in nature. The possibility of use of the selected bacterial strains reported, in the present study, in different sectors of the petroleum industry has been addressed.

  6. Characterization of biosurfactants produced by Lactobacillus spp. and their activity against oral streptococci biofilm.

    Science.gov (United States)

    Ciandrini, Eleonora; Campana, Raffaella; Casettari, Luca; Perinelli, Diego R; Fagioli, Laura; Manti, Anita; Palmieri, Giovanni Filippo; Papa, Stefano; Baffone, Wally

    2016-08-01

    Lactic acid bacteria (LAB) can interfere with pathogens through different mechanisms; one is the production of biosurfactants, a group of surface-active molecules, which inhibit the growth of potential pathogens. In the present study, biosurfactants produced by Lactobacillus reuteri DSM 17938, Lactobacillus acidophilus DDS-1, Lactobacillus rhamnosus ATCC 53103, and Lactobacillus paracasei B21060 were dialyzed (1 and 6 kDa) and characterized in term of reduction of surface tension and emulsifying activity. Then, aliquots of the different dialyzed biosurfactants were added to Streptococcus mutans ATCC 25175 and Streptococcus oralis ATCC 9811 in the culture medium during the formation of biofilm on titanium surface and the efficacy was determined by agar plate count, biomass analyses, and flow cytometry. Dialyzed biosurfactants showed abilities to reduce surface tension and to emulsifying paraffin oil. Moreover, they significantly inhibited the adhesion and biofilm formation on titanium surface of S. mutans and S. oralis in a dose-dependent way, as demonstrated by the remarkable decrease of cfu/ml values and biomass production. The antimicrobial properties observed for dialyzed biosurfactants produced by the tested lactobacilli opens future prospects for their use against microorganisms responsible of oral diseases.

  7. QFood - Optimal design of food products

    DEFF Research Database (Denmark)

    Bech, Anne C.; Engelund, Erling; Juhl, Hans Jørn

    1994-01-01

    of Quality is described with special reference to the development of food products. 5. An MDS-based model for use in the evaluation of an optimal product is developed. The model is based on the profit function from classical micro-economic theory. The imputed price is defined as a function of a Customer...... Satisfaction Index which is inversely proportional to how ""close"" the product is to the consumer's ideal....

  8. Characterization and properties of biosurfactants produced by a newly isolated strain Bacillus methylotrophicus DCS1 and their applications in enhancing solubility of hydrocarbon.

    Science.gov (United States)

    Jemil, Nawel; Ben Ayed, Hanen; Hmidet, Noomen; Nasri, Moncef

    2016-11-01

    Six biosurfactant-producing bacteria were isolated from hydrocarbon contaminated soils in Sfax, Tunisia. Isolates were screened for biosurfactant production by different conventional methods including hemolytic activity, surface tension reduction, drop-collapsing and oil displacement tests. All these screening tests show that all the isolates behave differently. Among the isolated bacteria, DCS1 strain was selected for further studies based on its highest activities and it was identified as Bacillus methylotrophicus DCS1. This strain was found to be a potent producer of biosurfactant when cultivated in mineral-salts medium supplemented with diesel oil (2 %, v/v) as a sole carbon source. Physicochemical properties and stability of biosurfactants synthesized by B. methylotrophicus DCS1 were investigated. The produced biosurfactants DCS1, from Landy medium, possess high surface activity that could lower the surface tension of water to a value of 31 from 72 mN m(-1) and have a critical micelle concentration (CMC) of 100 mg L(-1). Compared with SDS and Tween 80, biosurfactants showed excellent emulsification activities against different hydrocarbon substrates and high solubilization efficiency towards diesel oil. Biosurfactants DCS1 showed good stability in a wide range of temperature, pH and salinity. These results suggested that biosurfactants produced by B. methylotrophicus DCS1 could be an alternative to chemically synthesized surfactants for use in bioremediation processes to enhance the solubility of hydrophobic compounds.

  9. Halomonas sp. BS4, A biosurfactant producing halophilic bacterium isolated from solar salt works in India and their biomedical importance.

    Science.gov (United States)

    Donio, Mariathason Birdilla Selva; Ronica, Fernando Arul; Viji, Vijayaragavan Thanga; Velmurugan, Subramanian; Jenifer, John Selesteen Charles Adlin; Michaelbabu, Mariavincent; Dhar, Prasenjit; Citarasu, Thavasimuthu

    2013-12-01

    Halophilic bacteria were isolated from Thamaraikulam solar salt works in India. After routine biosurfactant screening by various methods, the biosurfactant producing bacteria, Halomonas sp BS4 was confirmed by 16 S rRNA sequencing. The growth optimization of Halomonas sp BS4 revealed their optimum growth at 8% NaCl and 6-8 pH in the growth medium. Further the partially purified biosurfactants were characterized by TLC, FTIR and GC-MS analysis. GC-MS results revealed that, the partial purified biosurfactants contain 1, 2-Ethanediamine N, N, N', N'-tetra, 8-Methyl-6-nonenamide, (Z)-9-octadecenamide and a fatty acid derivative. Pharmacological screening of antibacterial, antifungal, antiviral and anticancer assays revealed that, the biosurfactant extracted from Halomonas sp BS4 effectively controlled the human pathogenic bacteria and fungi an aquaculturally important virus, WSSV. The biosurfactant also suppressed the proliferation of mammary epithelial carcinoma cell by 46.77% at 2.5 μg concentration. Based on these findings, the present study concluded that, there is a possibility to develop eco-friendly antimicrobial and anticancer drugs from the extremophilic origin.

  10. Degradation of Polycyclic Aromatic Hydrocarbon Pyrene by Biosurfactant-Producing Bacteria Gordonia cholesterolivorans AMP 10

    Directory of Open Access Journals (Sweden)

    Tri Handayani Kurniati

    2016-12-01

    Full Text Available Pyrene degradation and biosurfactant activity by a new strain identified as Gordonia cholesterolivorans AMP 10 were studied. The strain grew well and produced effective biosurfactants in the presence of glucose, sucrose, and crude oil. The biosurfactants production was detected by the decreased surface tension of the medium and emulsification activity.  Analysis of microbial growth parameters showed that AMP10 grew best at 50 µg mL-1 pyrene concentration, leading to 96 % degradation of pyrene within 7 days. The result of nested PCR analysis revealed that this isolate possessed the nahAc gene which encodes dioxygenase enzyme for initial degradation of Polycyclic Aromatic Hydrocarbon (PAH. Observation of both tensio-active and emulsifying activities indicated that biosurfactants which produced by AMP 10 when grown on glucose could lower the surface tension of medium from 71.3 mN/m to 24.7 mN/m and formed a stable emulsion in used lubricant oil with an emulsification index (E24 of 74%. According to the results, it is suggested that the bacterial isolates G. cholesterolivorans AMP10 are suitable candidates for bioremediation of PAH-contaminated environments.How to CiteKurniati, T. H.,  Rusmana, I. Suryani, A. & Mubarik, N. R. (2016. Degradation of Polycyclic Aromatic Hydrocarbon Pyrene by Biosurfactant-Producing Bacteria Gordonia cholesterolivorans AMP 10. Biosaintifika: Journal of Biology & Biology Education, 8(3, 336-343. 

  11. Biomedical and therapeutic applications of biosurfactants

    OpenAIRE

    Rodrigues, L. R.; Teixeira, J. A.

    2010-01-01

    During the last years, several applications of biosurfactants with medical purposes have been reported. Biosurfactants are considered relevant molecules for applications in combating many diseases and as therapeutic agents due to their antibacterial, antifungal and antiviral activities. Furthermore, their role as anti-adhesive agents against several pathogens illustrate their utility as suitable anti-adhesive coating agents for medical insertional materials leading to a reduction of a large n...

  12. Evidence for a role of biosurfactants produced by Pseudomonas fluorescens in the spoilage of fresh aerobically stored chicken meat.

    Science.gov (United States)

    Mellor, Glen E; Bentley, Jessica A; Dykes, Gary A

    2011-08-01

    Fresh chicken meat is a fat-rich environment and we therefore hypothesised that production of biosurfactants to increase bioavailability of fats may represent one way in which spoilage bacteria might enhance the availability of nutrients. Numbers of Pseudomonas were determined on a total of 20 fresh and 20 spoiled chicken thighs with skin. A total of 400 randomly isolated Pseudomonas colonies from fresh (200) and spoiled (200) chicken were screened for the presence of biosurfactant production. Biosurfactant producing strains represented 5% and 72% of the Pseudomonas spp. isolates from fresh (mean count 2.3 log(10) cfu g(-1)) and spoiled (mean count 7.4 log(10) cfu g(-1)) chicken skin, respectively. Partially-purified biosurfactants derived from a subgroup of four Pseudomonasfluorescens strains obtained through the screening process were subsequently used to investigate the role that the addition of these compounds plays in the spoilage of aerobically stored chicken. Emulsification potential of the four selected biosurfactants was measured against a range of hydrocarbons and oils. All four biosurfactants displayed a greater ability to emulsify rendered chicken fat than hydrocarbons (paraffin liquid, toluene and hexane) and oils (canola, olive, sunflower and vegetable). Storage trials (4 °C) of chicken meat treated with the four selected biosurfactants revealed a significantly greater (P increase in total aerobic count (1.3-1.7 log(10) cfu g(-1)) occurred following one day of incubation. These results indicate that biosurfactants produced by Pseudomonas spp. may play an important role in the spoilage of aerobically stored chicken meat by making nutrients more freely available and providing strains producing them with a competitive advantage. Copyright © 2011 Elsevier Ltd. All rights reserved.

  13. A novel biosurfactant produced by Aureobasidium pullulans L3-GPY from a tiger lily wild flower, Lilium lancifolium Thunb.

    Science.gov (United States)

    Kim, Jong Shik; Lee, In Kyoung; Yun, Bong Sik

    2015-01-01

    Yeast biosurfactants are important biotechnological products in the food industry, and they have medical and cosmeceutical applications owing to their specific modes of action, low toxicity, and applicability. Thus, we have isolated and examined biosurfactant-producing yeast for various industrial and medical applications. A rapid and simple method was developed to screen biosurfactant-producing yeasts for high production of eco-friendly biosurfactants. Using this method, several potential niches of biosurfactant-producing yeasts, such as wild flowers, were investigated. We successfully selected a yeast strain, L3-GPY, with potent surfactant activity from a tiger lily, Lilium lancifolium Thunb. Here, we report the first identification of strain L3-GPY as the black yeast Aureobasidium pullulans. In addition, we isolated a new low-surface-tension chemical, designated glycerol-liamocin, from the culture supernatant of strain L3-GPY through consecutive chromatography steps, involving an ODS column, solvent partition, silica gel, Sephadex LH-20, and an ODS Sep-Pak cartridge column. The chemical structure of glycerol-liamocin, determined by mass spectrometry and nuclear magnetic resonance spectroscopy, indicates that it is a novel compound with the molecular formula C33H62O12. Furthermore, glycerol-liamocin exhibited potent biosurfactant activity (31 mN/m). These results suggest that glycerol-liamocin is a potential novel biosurfactantfor use in various industrial applications.

  14. Screening of potential biosurfactant-producing bacteria isolated from ...

    African Journals Online (AJOL)

    Seawater represents a specific environment harboring complex bacterial community which is adapted to harsh conditions. Hence, biosurfactant produced by these bacteria under these conditions have interesting proprieties. The screening of biosurfactant producing strains isolated from seawater biofilm was investigated.

  15. Optimization of mutanase production by Trichoderma harzianum ...

    African Journals Online (AJOL)

    PROMOTING ACCESS TO AFRICAN RESEARCH ... The highest enzyme activity (0.747 U/mL) was reached in shaken flask cultures ... increase in the production of mutanase compared with the culture before optimization. ... Keywords: Mutanase, Trichoderma harzianum, response surface methodology (RSM), bioreactors, ...

  16. Optimization of Product Instantiation using Integer Programming

    NARCIS (Netherlands)

    van den Broek, P.M.; Botterweck, Goetz; Jarzabek, Stan; Kishi, Tomoji

    2010-01-01

    We show that Integer Programming (IP) can be used as an optimization technique for the instantiation of products of feature models. This is done by showing that the constraints of feature models can be written in linear form. As particular IP technique, we use Gomory cutting planes. We have applied

  17. Optimal Product Variety, Scale Effects and Growth

    NARCIS (Netherlands)

    de Groot, H.L.F.; Nahuis, R.

    1997-01-01

    We analyze the social optimality of growth and product variety in a model of endogenous growth. The model contains two sectors, one assembly sector producing a homogenous consumption good, and one intermediate goods sector producing a differentiated input used in the assembly sector. Growth results

  18. Going Green and Cold: Biosurfactants from Low-Temperature Environments to Biotechnology Applications.

    Science.gov (United States)

    Perfumo, Amedea; Banat, Ibrahim M; Marchant, Roger

    2018-03-01

    Approximately 80% of the Earth's biosphere is cold, at an average temperature of 5°C, and is populated by a diversity of microorganisms that are a precious source of molecules with high biotechnological potential. Biosurfactants from cold-adapted organisms can interact with multiple physical phases - water, ice, hydrophobic compounds, and gases - at low and freezing temperatures and be used in sustainable (green) and low-energy-impact (cold) products and processes. We review the biodiversity of microbial biosurfactants produced in cold habitats and provide a perspective on the most promising future applications in environmental and industrial technologies. Finally, we encourage exploring the cryosphere for novel types of biosurfactants via both culture screening and functional metagenomics. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Genomic and functional features of the biosurfactant producing Bacillus sp. AM13.

    Science.gov (United States)

    Shaligram, Shraddha; Kumbhare, Shreyas V; Dhotre, Dhiraj P; Muddeshwar, Manohar G; Kapley, Atya; Joseph, Neetha; Purohit, Hemant P; Shouche, Yogesh S; Pawar, Shrikant P

    2016-09-01

    Genomic studies provide deeper insights into secondary metabolites produced by diverse bacterial communities, residing in various environmental niches. This study aims to understand the potential of a biosurfactant producing Bacillus sp. AM13, isolated from soil. An integrated approach of genomic and chemical analysis was employed to characterize the antibacterial lipopeptide produced by the strain AM13. Genome analysis revealed that strain AM13 harbors a nonribosomal peptide synthetase (NRPS) cluster; highly similar with known biosynthetic gene clusters from surfactin family: lichenysin (85 %) and surfactin (78 %). These findings were substantiated with supplementary experiments of oil displacement assay and surface tension measurements, confirming the biosurfactant production. Further investigation using LCMS approach exhibited similarity of the biomolecule with biosurfactants of the surfactin family. Our consolidated effort of functional genomics provided chemical as well as genetic leads for understanding the biochemical characteristics of the bioactive compound.

  20. Removal of PAH using electrokinetic transport of biosurfactants in clayey soil

    Energy Technology Data Exchange (ETDEWEB)

    Maria, E.; Lin, J. [Dept. of Building Civil and Environmental Engineering, Concordia Univ., Montreal (Canada)

    2001-07-01

    The electrokinetic introduction of non-toxic, biodegradable surfactants (produced ex-situ) to remediate PAH-contaminated soil was investigated. The lab tests demonstrated the possibility of removal of organic contaminants from clayey soil without hazardous impact to the environment. The rhamnolipids (biosurfactants), produced by Pseudomonas aeruginosa to increase the solubility of PAHs into the aqueous phase, were used in the enhancement of electrokinetic remediation. This study determined the potential on-site production of biosurfactants that was directly introduced to soil by means of electrokinetics. The average removal of phenanthrene achieved 74% in the presence of biosurfactants above CMC. The remaining compounds are left for biodegradation. These results contribute to the development of a new remediation technology - bioelectrokinetics. (orig.)

  1. Investigation on ultrasonication mediated biosurfactant disintegration method in sludge flocs for enhancing hydrolytic enzymes activity and polyhydroxyalkanoates.

    Science.gov (United States)

    Sethupathy, A; Sivashanmugam, P

    2018-06-04

    In this study, a novel biosurfactant potential bacterial strain Pseudomonas pachastrellae RW43 was isolated from pulp and paper sludge and the biosurfactant namely rhamnolipid produced by Pseudomonas pachastrellae RW43 was investigated by varying pH and incubation time in batch liquid fermentation process. The maximal yield of rhamnolipid was found to be 12.1 g/L at an optimized condition of pH 7 and incubation time of 168 h. NMR analysis was performed for identification of molecular structure of produced rhamnolipid and its results concluded that the product was identified as di rhamnolipid. Then, statistically the global optimum conditions for hydrolytic enzymes extraction parameters (sonication power (100 W), extraction time (15 min) and rhamnolipid dosage (2% v/v)) were established. At 30,456 kJ/kg TS specific energy, ultrasonication with rhamnolipid disintegration method extracted maximal consortium activity of hydrolytic enzymes from mixed sludge (municipal and pulp & paper sludge) and the maximum observed were found to be 42.22, 51.75, 34.26, 24.21, 11.35 Units/g VSS respectively for protease, α-amylase, cellulase, lipase and α-glucosidase. Polyhydroxyalkanoates was recovered from enzymes extracted sludge using various solvents namely chloroform, sodium hypochlorite with chloroform and sodium lauryl sulfate with sodium hypochlorite. The maximum recovery was found to be 74 g/kg using sodium hypochlorite and chloroform extraction solvents.

  2. Antimicrobial and Anti-Swarming Effects of Bacteriocins and Biosurfactants from Probiotic Bacterial Strains against Proteus spp.

    Directory of Open Access Journals (Sweden)

    Laila Goudarzi

    2017-02-01

    Full Text Available Background:   Proteus spp. belongs to the family of Enterobacteriaceae. These bacteria are Gram-negative and motile microorganisms and known as the third most common causes of urinary tract infections. The aim of the current study was to investigate the effects of some secondary metabolites from probiotic strains of Lactobacillus spp. on swarming and growth of Proteus mirabilis and P. vulgaris. Methods:   After determination of optimal conditions for the growth and production of antimicrobials, bacteriocins and biosurfactants were partially purified from Lactobacillus culture supernatants. Then, effects of the purified compounds on growth and swarming migration of Proteus spp. were examined in the presence of various concentrations of semi-purified compounds. Results:  Results showed that the partially purified bacteriocins inhibited Proteus spp. swarming distance and had a significant reduction on the bacterial growth curves. Biosurfactants in a solvent form did not have any considerable effects on factors produced by Proteus spp. Conclusion:  According to the results, the secondary metabolites, especially bacteriocins or bacteriocin-like substances derived from Lactobacillus strains, can inhibit or reduce growth and swarming migration of Proteus spp. which are considered as the bacteria major virulence factors.

  3. Optimization of Bioethanol Production from Coffee Mucilage

    Directory of Open Access Journals (Sweden)

    Antonio De León-Rodríguez

    2015-05-01

    Full Text Available A response surface methodology with 2k full factorial design was applied to obtain optimum conditions for bioethanol production using coffee mucilage (CM as the substrate and Saccharomyces cerevisiae NRRL Y-2034 as the inoculum. CM is an agro-industrial residue mainly composed of simple sugars; the product yield and productivity process were analyzed with respect to the fermentation, pH, temperature, and the initial sugar concentration. Employing the following predicted optimum operational conditions attained the highest bioethanol production: pH 5.1, temperature 32 °C, and initial sugar concentration 61.8 g/L. The estimated bioethanol production was 15.02 g/L, and the experimental production was 16.29 g/L ± 0.39 g/L, with a bioethanol yield of 0.27 g/L and a productivity process of 0.34 g/Lh. Glycerol was the predominant byproduct of the fermentative metabolism of S. cerevisiae. The response surface methodology was successfully employed to optimize CM fermentation. In the fermentative processes with yeast, optimizing the conditions of the culture medium is needed to fully exploit the potential of the strains and maximize the production of bioethanol.

  4. Isolation and functional characterization of a biosurfactant produced by a new and promising strain of Oleomonas sagaranensis AT18.

    Science.gov (United States)

    Saimmai, Atipan; Rukadee, Onkamon; Onlamool, Theerawat; Sobhon, Vorasan; Maneerat, Suppasil

    2012-10-01

    Biosurfactant-producing bacteria were isolated from mangrove sediment in southern Thailand. Isolates were screened for biosurfactant production by using the surface tension test. The highest reduction of surface tension was achieved with a bacterial strain which was identified by 16S rRNA gene sequencing as Oleomonas sagaranensis AT18. It has also been investigated using different carbon and nitrogen sources. It showed that the strain was able to grow and reduce the surface tension of the culture supernatant to 25 mN/m. In all 5.30 g of biosurfactant yield was obtained after 54 h of cultivation by using molasses and NaNO₃ as carbon and nitrogen sources, respectively. The biosurfactant recovery by chloroform:methanol extraction showed a small critical micelle concentration value (8 mg/l), thermal and pH stability with respect to surface tension reduction. It also showed emulsification activity and a high level of salt concentration. The biosurfactant obtained was confirmed as a glycolipid by using a biochemical test, FT-IR and mass spectra. The crude biosurfactant showed a broad spectrum of antimicrobial activity and also had the ability to emulsify oil and enhance PAHs solubility.

  5. Effect of biosurfactant from two strains of Pseudomonas on ...

    African Journals Online (AJOL)

    Two Pseudomonas strains isolated from oil-contaminated soil which produce biosurfactant were studied. The biosurfactant containing broth formed stable emulsions with liquid light paraffin, cooking medium vegetable oil and toluene. The strains under study produce extra cellular biosurfactant in the culture media.

  6. High-Level Culturability of Epiphytic Bacteria and Frequency of Biosurfactant Producers on Leaves

    Science.gov (United States)

    Burch, Adrien Y.; Do, Paulina T.; Sbodio, Adrian; Suslow, Trevor V.

    2016-01-01

    ABSTRACT To better characterize the bacterial community members capable of biosurfactant production on leaves, we distinguished culturable biosurfactant-producing bacteria from nonproducers and used community sequencing to compare the composition of these distinct cultured populations with that from DNA directly recovered from leaves. Communities on spinach, romaine, and head lettuce leaves were compared with communities from adjacent samples of soil and irrigation source water. Soil communities were poorly described by culturing, with recovery of cultured representatives from only 21% of the prevalent operational taxonomic units (OTUs) (>0.2% reads) identified. The dominant biosurfactant producers cultured from soil included bacilli and pseudomonads. In contrast, the cultured communities from leaves are highly representative of the culture-independent communities, with over 85% of the prevalent OTUs recovered. The dominant taxa of surfactant producers from leaves were pseudomonads as well as members of the infrequently studied genus Chryseobacterium. The proportions of bacteria cultured from head lettuce and romaine leaves that produce biosurfactants were directly correlated with the culture-independent proportion of pseudomonads in a given sample, whereas spinach harbored a wider diversity of biosurfactant producers. A subset of the culturable bacteria in irrigation water also became enriched on romaine leaves that were irrigated overhead. Although our study was designed to identify surfactant producers on plants, we also provide evidence that most bacteria in some habitats, such as agronomic plant surfaces, are culturable, and these communities can be readily investigated and described by more classical culturing methods. IMPORTANCE The importance of biosurfactant production to the bacteria that live on waxy leaf surfaces as well as their ability to be accurately assessed using culture-based methodologies was determined by interrogating epiphytic populations by

  7. Structural Characterization and Antimicrobial Activity of a Biosurfactant Obtained From Bacillus pumilus DSVP18 Grown on Potato Peels

    Science.gov (United States)

    Sharma, Deepak; Ansari, Mohammad Javed; Gupta, Sonam; Al Ghamdi, Ahmad; Pruthi, Parul; Pruthi, Vikas

    2015-01-01

    Background: Biosurfactants constitute a structurally diverse group of surface-active compounds derived from microorganisms. They are widely used industrially in various industrial applications such as pharmaceutical and environmental sectors. Major limiting factor in biosurfactant production is their production cost. Objectives: The aim of this study was to investigate biosurfactant production under laboratory conditions with potato peels as the sole source of carbon source. Materials and Methods: A biosurfactant-producing bacterial strain (Bacillus pumilus DSVP18, NCBI GenBank accession no. GQ865643) was isolated from motor oil contaminated soil samples. Biochemical characteristics of the purified biosurfactant were determined and its chemical structure was analyzed. Stability studies were performed and biological activity of the biosurfactant was also evaluated. Results: The strain, when grown on modified minimal salt media supplemented with 2% potato peels as the sole carbon source, showed the ability to reduce Surface Tension (ST) value of the medium from 72 to 28.7 mN/m. The isolated biosurfactant (3.2 ± 0.32 g/L) was stable over a wide range of temperatures (20 - 120 ºC), pH (2-12) and salt concentrations (2 - 12%). When characterized using high-performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy, it was found to be a lipopeptide in nature, which was further confirmed by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (mass peak 1044.60) and nuclear magnetic resonance (NMR) studies. Data showed that the isolated biosurfactant at the concentration range of 30 - 35 µg/ml had strong antimicrobial activity when tested against standard strains of Bacillus cereus, Escherichia coli, Salmonella enteritidis, Staphylococcus aureus and Paenibacillus larvae. Conclusions: Potato peels were proved to be potentially useful substrates for biosurfactant production by B. pumilus DSVP18. The strain possessed a

  8. [Optimization of formulations for dietetic pastry products].

    Science.gov (United States)

    Villarroel, M; Uquiche, E; Brito, G; Cancino, M

    2000-03-01

    Optimized formulations of dietetic pastry products such as cake and sponge cake premixes were formulated using the surface response methodology. % Emulsifier agent and baking time were the selected independent variables for cake, as well as % emulsifier agent % chlorinated flour the variables selected for sponge cake. Three different level of each variable summing up thirteen experimental formulae of each product were assessed to optimize the variables that could have some influence in the sensory characteristics of these dietetic products. The total sensory quality was determined for both dietetic products using the composite scoring test and a panel of 18 trained judges. Looking at the contour graphic and considering economic aspects the best combination of variables for cake formulation was 2% emulsifier agent and 48 minutes for baking time, With respect to sponge cake, the best combination was 6% emulsifier agent and 48% chlorinated flour. Shelf life studies showed that both dietetic formulations remained stable during storage conditions of 75 days at 30 degrees C. During this period, significant differences in sensory characteristics were not found (p pastry products had good acceptability, and open up marketing opportunities for new products with potential health benefits to consumers.

  9. Biogeographical distribution analysis of hydrocarbon degrading and biosurfactant producing genes suggests that near-equatorial biomes have higher abundance of genes with potential for bioremediation

    OpenAIRE

    Oliveira, Jorge S.; Ara?jo, Wydemberg J.; Figueiredo, Ricardo M.; Silva-Portela, Rita C. B.; de Brito Guerra, Alaine; da Silva Ara?jo, Sinara Carla; Minnicelli, Carolina; Carlos, Aline Cardoso; de Vasconcelos, Ana Tereza Ribeiro; Freitas, Ana Teresa; Agnez-Lima, Lucymara F.

    2017-01-01

    Background Bacterial and Archaeal communities have a complex, symbiotic role in crude oil bioremediation. Their biosurfactants and degradation enzymes have been in the spotlight, mainly due to the awareness of ecosystem pollution caused by crude oil accidents and their use. Initially, the scientific community studied the role of individual microbial species by characterizing and optimizing their biosurfactant and oil degradation genes, studying their individual distribution. However, with the...

  10. An Optimization Model for Product Placement on Product Listing Pages

    Directory of Open Access Journals (Sweden)

    Yan-Kwang Chen

    2014-01-01

    Full Text Available The design of product listing pages is a key component of Website design because it has significant influence on the sales volume on a Website. This study focuses on product placement in designing product listing pages. Product placement concerns how venders of online stores place their products over the product listing pages for maximization of profit. This problem is very similar to the offline shelf management problem. Since product information sources on a Web page are typically communicated through the text and image, visual stimuli such as color, shape, size, and spatial arrangement often have an effect on the visual attention of online shoppers and, in turn, influence their eventual purchase decisions. In view of the above, this study synthesizes the visual attention literature and theory of shelf-space allocation to develop a mathematical programming model with genetic algorithms for finding optimal solutions to the focused issue. The validity of the model is illustrated with example problems.

  11. Synthesis, characterization, and oil recovery application of biosurfactant produced by indigenous pseudomonas aeruginosa WJ-1 using waste vegetable oils.

    Science.gov (United States)

    Xia, Wen-Jie; Luo, Zhi-Bin; Dong, Han-Ping; Yu, Li; Cui, Qing-Feng; Bi, Yong-Qiang

    2012-03-01

    A bacterial strain was isolated and cultured from the oil excavation areas in tropical zone in northern China. The biochemical characteristics and partial sequenced 16S rRNA gene of isolate, WJ-1, was identical to those of cultured representatives of the species Pseudomonas aeruginosa. This bacterium was able to produce a type of biosurfactant. Compositional analysis revealed that the extracted biosurfactant was composed of high percentage lipid (∼74%, w/w) and carbohydrate (∼20%, w/w) in addition to a minor fraction of protein (∼6%, w/w). The best production of 50.2 g/l was obtained when the cells were grown on minimal salt medium containing 6.0% (w/v) glucose and 0.75% (w/v) sodium nitrate supplemented with 0.1% (v/v) element solution at 37 °C and 180 rpm after 96 h. The optimum biosurfactant production pH value was found to be 6.0-8.0. The biosurfactant of WJ-1, with the critical micelle concentration of 0.014 g/L, could reduce surface tension to 24.5 mN/m and emulsified kerosene up to EI(24) ≈95. The results obtained from time course study indicated that the surface tension reduction and emulsification potential was increased in the same way to cell growth. However, maximum biosurfactant production occurred and established in the stationary growth phase (after 90 h). Thin layer chromatography, Fourier transform infrared spectrum, and mass spectrum analysis indicate the extracted biosurfactant was affiliated with rhamnolipid. The core holder flooding experiments demonstrated that the oil recovery efficiency of strain and its biosurfactant was 23.02% residual oil.

  12. Isolation and characterization of biosurfactant/bioemulsifier-producing bacteria from petroleum contaminated sites.

    Science.gov (United States)

    Batista, S B; Mounteer, A H; Amorim, F R; Tótola, M R

    2006-04-01

    Biosurfactant-producing bacteria were isolated from terrestrial and marine samples collected in areas contaminated with crude oil or its byproducts. Isolates were screened for biosurfactant/bioemulsifier production in different carbon sources (glucose, fructose, sucrose and kerosene) using the qualitative drop-collapse test. Glucose produced the highest number of positive results (17 of 185 isolates). All 17 isolates produced emulsions with kerosene and 12 exhibited high emulsion-stabilizing capacity, maintaining 50% of the original emulsion volume for 48 h. Eight of the 17 isolates reduced the growth medium surface tension below 40 mN m(-1) with 5 exhibiting this capacity in cell-free filtrates. Onset of biosurfactant production differed among the isolates, with some initiating synthesis during the exponential growth phase and others after the stationary phase was reached. Increasing temperature from 25 to 35 degrees C accelerated onset of biosurfactant production in only two isolates while pH (6.5-7.6) had no effect in any isolate tested. Isolation from petroleum contaminated sites using the screening protocol presented proved to be a rapid and effective manner to identify bacterial isolates with potential industrial applications.

  13. Microbial biofilms: biosurfactants as antibiofilm agents.

    Science.gov (United States)

    Banat, Ibrahim M; De Rienzo, Mayri A Díaz; Quinn, Gerry A

    2014-12-01

    Current microbial inhibition strategies based on planktonic bacterial physiology have been known to have limited efficacy on the growth of biofilm communities. This problem can be exacerbated by the emergence of increasingly resistant clinical strains. All aspects of biofilm measurement, monitoring, dispersal, control, and inhibition are becoming issues of increasing importance. Biosurfactants have merited renewed interest in both clinical and hygienic sectors due to their potential to disperse microbial biofilms in addition to many other advantages. The dispersal properties of biosurfactants have been shown to rival those of conventional inhibitory agents against bacterial and yeast biofilms. This makes them suitable candidates for use in new generations of microbial dispersal agents and for use as adjuvants for existing microbial suppression or eradication strategies. In this review, we explore aspects of biofilm characteristics and examine the contribution of biologically derived surface-active agents (biosurfactants) to the disruption or inhibition of microbial biofilms.

  14. An efficient thermotolerant and halophilic biosurfactant-producing bacterium isolated from Dagang oil field for MEOR application

    Science.gov (United States)

    Wu, Langping; Richnow, Hans; Yao, Jun; Jain, Anil

    2014-05-01

    Dagang Oil field (Petro China Company Limited) is one of the most productive oil fields in China. In this study, 34 biosurfactant-producing strains were isolated and cultured from petroleum reservoir of Dagang oil field, using haemolytic assay and the qualitative oil-displacement test. On the basis of 16S rDNA analysis, the isolates were closely related to the species in genus Pseudomonas, Staphylococcus and Bacillus. One of the isolates identified as Bacillus subtilis BS2 were selected for further study. This bacterium was able to produce a type of biosurfactant with excessive foam-forming properties at 37ºC as well as at higher temperature of 55ºC. The biosurfactant produced by the strain BS2 could reduce the surface tension of the culture broth from 70.87 mN/m to 28.97 mN/m after 8 days of incubation at 37ºC and to 36.15 mN/m after 20 days of incubation at 55ºC, respectively. The biosurfactant showed stability at high temperature (up to 120ºC), a wide range of pH (2 to 12) and salt concentrations (up to 12%) offering potential for biotechnology. Fourier transform infrared (FT-IR) spectrum of extracted biosurfactant tentatively characterized the produced biosurfactant as glycolipid derivative. Elemental analysis of the biosurfactant by energy dispersive X-ray spectroscopy (EDS) reveals that the biosurfactant was anionic in nature. 15 days of biodegradation of crude oil suggested a preferential usage of n-alkane upon microbial metabolism of BS2 as a carbon substrate and consequently also for the synthesis of biosurfactants. Core flood studies for oil release indicated 9.6% of additional oil recovery over water flooding at 37ºC and 7.2% of additional oil recovery at 55 ºC. Strain BS2 was characterized as an efficient biosurfactant-producing, thermotolerant and halophillic bacterium and has the potential for application for microbial enhanced oil recovery (MEOR) through water flooding in China's oil fields even in situ as adapted to reservoir chemistry and

  15. Asset management: integrated software optimizes production performance

    Energy Technology Data Exchange (ETDEWEB)

    Polczer, S.

    1998-06-01

    Two new multi-dimensional databases, which expand the `row and column` concept of spreadsheets into multiple categories of data called dimensions, are described. These integrated software packages provide the foundation for industry players such as Poco Petroleum Ltd and Numac Energy Inc to gain a competitive advantage, by overhauling their respective data collection and retrieval systems to allow for timely cost analysis and financial reporting. Energy Warehouse, an on-line analytical processing product marketed by SysGold Ltd, is one of the software products described. It gathers various sources of information, allows advanced searches and generates reports previously unavailable in other conventional financial accounting systems. The second product discussed - the Canadian Upstream Energy System (CUES) - is an on-line analytical processing system developed by Oracle Corporation and Calgary-based Applied Terravision Systems (ATS) Inc. CUES combines Oracle`s universal data server and software development tools with ATS`s upstream financial, land, geotechnical and production applications. The software also allows for optimization of facilities, analysis of production efficiencies and comparison of performance against industry standards.

  16. Methods for investigating biosurfactants and bioemulsifiers: a review.

    Science.gov (United States)

    Satpute, Surekha K; Banpurkar, Arun G; Dhakephalkar, Prashant K; Banat, Ibrahim M; Chopade, Balu A

    2010-06-01

    Microorganisms produce biosurfactant (BS)/bioemulsifier (BE) with wide structural and functional diversity which consequently results in the adoption of different techniques to investigate these diverse amphiphilic molecules. This review aims to compile information on different microbial screening methods, surface active products extraction procedures, and analytical terminologies used in this field. Different methods for screening microbial culture broth or cell biomass for surface active compounds production are also presented and their possible advantages and disadvantages highlighted. In addition, the most common methods for purification, detection, and structure determination for a wide range of BS and BE are introduced. Simple techniques such as precipitation using acetone, ammonium sulphate, solvent extraction, ultrafiltration, ion exchange, dialysis, ultrafiltration, lyophilization, isoelectric focusing (IEF), and thin layer chromatography (TLC) are described. Other more elaborate techniques including high pressure liquid chromatography (HPLC), infra red (IR), gas chromatography-mass spectroscopy (GC-MS), nuclear magnetic resonance (NMR), and fast atom bombardment mass spectroscopy (FAB-MS), protein digestion and amino acid sequencing are also elucidated. Various experimental strategies including static light scattering and hydrodynamic characterization for micelles have been discussed. A combination of various analytical methods are often essential in this area of research and a numbers of trials and errors to isolate, purify and characterize various surface active agents are required. This review introduces the various methodologies that are indispensable for studying biosurfactants and bioemulsifiers.

  17. Optimization of cellulase production by Penicillium sp.

    Science.gov (United States)

    Prasanna, H N; Ramanjaneyulu, G; Rajasekhar Reddy, B

    2016-12-01

    The production of cellulolytic enzymes (β-exoglucanase, β-endoglucanase and β-glucosidase) by Penicillium sp. on three different media in liquid shake culture conditions was compared. The organism exhibited relatively highest activity of endoglucanase among three enzymes measured at 7-day interval during the course of its growth on Czapek-Dox medium supplemented with 0.5 % (w/v) cellulose. Cellulose at 0.5 %, lactose at 0.5 %, sawdust at 0.5 %, yeast extract at 0.2 % as a nitrogen source, pH 5.0 and 30 °C temperature were found to be optimal for growth and cellulase production by Penicillium sp. Yields of Fpase, CMCase and β-glucosidase, attained on optimized medium with Penicillium sp. were 8.7, 25 and 9.52 U/ml, respectively with increment of 9.2, 5.9 and 43.8-folds over titers of the respective enzyme on unoptimised medium. Cellulase of the fungal culture with the ratio of β-glucosidase to Fpase greater than one will hold potential for biotechnological applications.

  18. PRODUCT OPTIMIZATION METHOD BASED ON ANALYSIS OF OPTIMAL VALUES OF THEIR CHARACTERISTICS

    Directory of Open Access Journals (Sweden)

    Constantin D. STANESCU

    2016-05-01

    Full Text Available The paper presents an original method of optimizing products based on the analysis of optimal values of their characteristics . Optimization method comprises statistical model and analytical model . With this original method can easily and quickly obtain optimal product or material .

  19. Bioproduction and anticancer activity of biosurfactant produced by the dematiaceous fungus Exophiala dermatitidis SK80.

    Science.gov (United States)

    Chiewpattanakul, Paramaporn; Phonnok, Sirinet; Durand, Alain; Marie, Emmanuelle; Thanomsub, Benjamas Wongsatayanon

    2010-12-01

    A new biosurfactant producer was isolated from palm-oilcontaminated soil and later identified through morphology and DNA sequencing as the yeast-like fungus Exophiala dermatitidis. Biosurfactant production was catalyzed by vegetable oil, supplemented with a basal medium. The culture conditions that provided the biosurfactant with the highest surface activity were found to be 5% palm oil with 0.08% NH4NO3, at a pH of 5.3, with shaking at 200 rpm, and a temperature of 30 degrees C for a 14-day period of incubation. The biosurfactant was purified, in accordance with surfactant properties, by solvent fractionation using silica gel column chromatography. The chemical structure of the strongest surface-active compound was elucidated through the use of NMR and mass spectroscopy, and noted to be monoolein, which then went on to demonstrate antiproliferative activity against cervical cancer (HeLa) and leukemia (U937) cell lines in a dose-dependent manner. Interestingly, no cytotoxicity was observed with normal cells even when high concentrations were used. Cell and DNA morphological changes, in both cancer cell lines, were observed to be cell shrinkage, membrane blebbling, and DNA fragmentation.

  20. Screening of novel microorganisms for biosurfactant and biocontrol activity against Phytophthora infestans.

    Science.gov (United States)

    Tomar, Sonica; Singh, B P; Lal, Mehi; Ma, Khan; Hussain, Touseef; Sharma, Sanjeev; Kaushik, S K; Kumar, Satish

    2014-09-01

    In the present study, 95 isolates of bacteria were tested for their biosurfactant as well as biocontrol activity against Phytophthora infestans. The results revealed that only 15.8% isolates showed biosurfactant activity. The emulsification index ranged from 0-68% and 24.2% isolates showed positive reaction for biosurfactant properties. In emulsification assay and oil spreading test, 18.95% and 5.26% isolates, respectively scored positive for biosurfactant production. Among all, only five isolates were found effective against P. infestans, for biocontrol properties. Pseudomonas aeruginosa-1 showed 62.22% inhibition zone after 72 hrs while P. aeruginosa-3 showed 46.42%. Forty-eight hrs old culture supernatants were highly effective in food-poisoning test, tuber slice test and detached leaf method against P. infestans. In whole potato plant test, bacterial cell based formulation, culture supernatant and bacterial cell suspension of P. aeruginosa-1 showed 10.42%, 9.94% and 17.96% diseases severity respectively, as against 53.96% in control. This isolate holds promise as biological control agent against P. infestans in field.

  1. Screening of biosurfactant producers from petroleum hydrocarbon contaminated sources in cold marine environments.

    Science.gov (United States)

    Cai, Qinhong; Zhang, Baiyu; Chen, Bing; Zhu, Zhiwen; Lin, Weiyun; Cao, Tong

    2014-09-15

    An overview of literature about isolating biosurfactant producers from marine sources indicated no such producers have been reported form North Atlantic Canada. Water and sediment samples were taken from petroleum hydrocarbon contaminated coastal and offshore areas in this region. Either n-hexadecane or diesel was used as the sole carbon source for the screening. A modified colony-based oil drop collapsing test was used to cover sessile biosurfactant producers. Fifty-five biosurfactant producers belong to genera of Alcanivorax, Exiguobacterium, Halomonas, Rhodococcus, Bacillus, Acinetobacter, Pseudomonas, and Streptomyces were isolated. The first three genera were established after 1980s with interesting characteristics and limited relevant publications. Some of the 55 isolated strains were found with properties such as greatly reducing surface tension, stabilizing emulsion and producing flocculant. Isolates P6-4P and P1-5P were selected to demonstrate the performance of biosurfactant production, and were found to reduce the surface tension of water to as low as 28 dynes/cm. Copyright © 2014 Elsevier Ltd. All rights reserved.

  2. Biosurfactants in Dwarf Mistletoe ( Arceuthobium ) | Vaudry | Plant ...

    African Journals Online (AJOL)

    0.01), reflects a drop in water surface tension from 72 mN/m to under 30 mN/m, a purification factor of 575-fold, and a significantly more active (p<0.05) purified sample relative to the crude. The biosurfactant has a critical micellar concentration ...

  3. Characterization of biosurfactant produced from submerged ...

    African Journals Online (AJOL)

    user

    interfacial tension. This work was designed to produce biosurfactants from the fermentation of .... The cashew apples were collected from. Ubogidi cashew ... and manually crushed to remove the juice using a pestle and mortar. The bagasse was ..... degradation by yeast species Trichosporon asahii isolated from petroleum ...

  4. Characterization of biosurfactants produced by novel strains of Ochrobactrum anthropi HM-1 and Citrobacter freundii HM-2 from used engine oil-contaminated soil

    Directory of Open Access Journals (Sweden)

    Haytham M.M. Ibrahim

    2018-03-01

    Full Text Available Microbial surfactants are widely used for industrial, agricultural, food, cosmetics, pharmaceutical, and medical applications. In this study, two bacterial strains namely, Ochrobactrum anthropi HM-1 and Citrobacter freundii HM-2, previously isolated from used engine oil contaminated soil, and capable of producing biosurfactants, were used. Their cell-free culture broth showed positive results toward five screening tests (hemolysis in blood agar, drop collapse, oil displacement, emulsification activity (E24, and surface tension (ST reduction. They reduced the ST of growth medium (70 ± 0.9 to 30.8 ± 0.6 and 32.5 ± 1.3 mN/m, respectively. The biosurfactants were classified as anionic biomolecules. Based on TLC pattern and FT-IR analysis, they were designated as glycolipids (rhamnolipid. Waste frying oil was feasibly used as a cheap and dominant carbon source for biosurfactants production; 4.9 and 4.1 g/l were obtained after 96 h of incubation, respectively. Compared with non-irradiated cells, gamma-irradiated cells (1.5 kGy revealed enhanced biosurfactant production by 56 and 49% for HM-1 and HM-2, respectively. The biosurfactants showed good stability after exposure to extreme conditions [temperatures (50–100 °C for 30 min, pH (2–12 and salinity (2–10% NaCl], they retained 83 and 79.3% of their E24, respectively, after incubation for a month, under extreme conditions. Biosurfactants effectively recovered up to 70 and 67% of the residual oil, respectively, from oil-saturated sand pack columns. These biosurfactants are an interesting biotechnological product for many environmental and industrial applications. Keywords: Ochrobactrum anthropi, Citrobacter freundii, Biosurfactant, Characterization, Stability

  5. Characterization of biosurfactants produced by the oil-degrading bacterium Rhodococcus erythropolis S67 at low temperature.

    Science.gov (United States)

    Luong, T M; Ponamoreva, O N; Nechaeva, I A; Petrikov, K V; Delegan, Ya A; Surin, A K; Linklater, D; Filonov, A E

    2018-01-04

    Production of trehalolipid biosurfactants by Rhodococcus erythropolis S67 depending on the growth temperature was studied. R. erythropolis S67 produced glycolipid biosurfactants such as 2,3,4-succinoyl-octanoyl-decanoyl-2'-decanoyl trehalose and 2,3,4-succinoyl-dioctanoyl-2'-decanoyl trehalose during the growth in n-hexadecane medium at 26 and 10 °C, despite the different aggregate state of the hydrophobic substrate at low temperature. The surface tension of culture medium was found being reduced from 72 to 27 and 45 mN m -1 , respectively. Production of trehalolipid biosurfactants by R. erythropolis S67 at low temperature could be useful for the biodegradation of petroleum hydrocarbons at low temperatures by enhancing the bioremediation performance in cold regions.

  6. Contributions of biosurfactants to natural or induced bioremediation.

    Science.gov (United States)

    Lawniczak, Lukasz; Marecik, Roman; Chrzanowski, Lukasz

    2013-03-01

    The number of studies dedicated to evaluating the influence of biosurfactants on bioremediation efficiency is constantly growing. Although significant progress regarding the explanation of mechanisms behind biosurfactant-induced effects could be observed, there are still many factors which are not sufficiently elucidated. This corresponds to the fact that although positive influence of biosurfactants is often reported, there are also numerous cases where no or negative effect was observed. This review summarizes the recent finding in the field of biosurfactant-amended bioremediation, focusing mainly on a critical approach towards potential limitations and causes of failure while investigating the effects of biosurfactants on the efficiency of biodegradation and phytoextraction processes. It also provides a summary of successive steps, which should be taken into consideration when designing biosurfactant-related treatment processes.

  7. Kanban simulation model for production process optimization

    Directory of Open Access Journals (Sweden)

    Golchev Riste

    2015-01-01

    Full Text Available A long time has passed since the KANBAN system has been established as an efficient method for coping with the excessive inventory. Still, the possibilities for its improvement through its integration with other different approaches should be investigated further. The basic research challenge of this paper is to present benefits of KANBAN implementation supported with Discrete Event Simulation (DES. In that direction, at the beginning, the basics of KANBAN system are presented with emphasis on the information and material flow, together with a methodology for implementation of KANBAN system. Certain analysis on combining the simulation with this methodology is presented. The paper is concluded with a practical example which shows that through understanding the philosophy of the implementation methodology of KANBAN system and the simulation methodology, a simulation model can be created which can serve as a basis for a variety of experiments that can be conducted within a short period of time, resulting with production process optimization.

  8. An efficient biosurfactant-producing bacterium Selenomonas ruminantium CT2, isolated from mangrove sediment in south of Thailand.

    Science.gov (United States)

    Saimmai, Atipan; Onlamool, Theerawat; Sobhon, Vorasan; Maneerat, Suppasil

    2013-01-01

    Biosurfactant-producing bacteria, isolate CT2, was isolated from mangrove sediment in the south of Thailand. The sequence of the 16S rRNA gene from isolate CT2 showed 100 % similarity with Selenomonas ruminantium. The highest biosurfactant production (5.02 g/l) was obtained when the cells were grown on minimal salt medium containing 15 g/l molasses and 1 g/l commercial monosodium glutamate supplemented with 1 g/l NaCl, 0.1 g/l leucine, 5 % (v/v) inoculum size at 30 °C and 150 rpm after 54 h of cultivation. The biosurfactant obtained by extraction with ethyl acetate showed high surface tension reduction (25.5 mN/m), a small CMC value (8 mg/l), thermal and pH stability with respect to surface tension reduction and emulsification activity and a high level of salt tolerance. The biosurfactant obtained was confirmed as a lipopeptide by using a biochemical test, FT-IR, MNR and mass spectrometry. The crude biosurfactant showed a broad spectrum of antimicrobial activity and also had the ability to emulsify oil and enhance PAHs solubility.

  9. Biosurfactants produced by Microbacterium sp., isolated from aquatic macrophytes in hydrocarbon-contaminated area in the Rio Negro, Manaus, Amazonas

    Directory of Open Access Journals (Sweden)

    João Marcelo Silva Lima

    2017-05-01

    Full Text Available Endophytic bacteria isolated from Eichhornia crassipes (Mart Solms., collected in oil contaminated wastewater of effluent generated by Petrobras refinery in Manaus were investigated to determine their potential for producing biosurfactants. Assay with 2.6-dichlorophenol indophenol (DCPIP indicator to verify hydrocarbon biodegradation activity; oil emulsification test; drop-collapse method; surface tension and growth curve of biosurfactant production. The M87 Microbacterium sp. strain chosen for this work was identified by the sequencing of the rDNA region and the chemical characterization was performed by FTIR, UFLC/MS and 1H RMN techniques. The selected bacterial isolate provided 3g L-1 of biosurfactant, using diesel oil as sole carbon source, being efficient in biodegrading oil as demonstrated by the DCPIP test. Fractions obtained by column chromatography were efficient in reducing water surface tension around 40 mN m-1, especially fraction 1, which reduced it to 34.17 mN m-1. The different techniques of chemical analysis used for the identification of the biosurfactant isolate indicated that this is probably a long - chain fatty acid lipid type, which may be used in the future as both biosurfactant in decontamination processes of hydrocarbon-polluted areas or as bioemulsifier in countless processes, since it exhibited no toxicity as determined by Alamar Blue assay.

  10. Principles of optimizing animal production from rangeland

    International Nuclear Information System (INIS)

    Stubbendieck, J.; Waller, S.S.

    1983-01-01

    Increasing world population is one of the dominant factors escalating demands for the world's natural resources. Range and forage resources, which are used primarily for food and fibre, could be more efficiently used if management techniques were improved. The principles of managing forage resources are directly associated with both the growth and development of plants and the actions and needs of the grazing animal. An understanding of the effects of environmental factors and herbage removal (frequency, intensity and season of defoliation) on growth and regrowth of plants is the first step towards optimizing animal productivity from rangelands. Most potential changes will fit into three categories: (1) increase the quantity of forage, (2) improve the quality of forage, and (3) improve use of forage. The principles of grazing management can be separated into four intricately related categories: (1) proper degree of grazing, (2) proper season of grazing, (3) proper kind of livestock, and (4) proper distribution of grazing. Grazing management is affected by the manner in which both improvements and manipulation of vegetation affect forage yield and quality. The adaptation and application of existing knowledge to individual locations will be one step towards optimizing animal production from rangeland. Some of the problems may be solved through better dissemination of present knowledge through existing educational programmes, while others will require expanded programmes of information dissemination. A third group of problems may also be solved with present technology, but the solutions are not currently economical. Some of the problems will be solved only through expanded research. These research efforts need to be directed towards grazing or browsing animals, plant resources and the interaction between plants and animals. Application of nuclear techniques will be an integral part of this research. (author)

  11. Interactions between biosurfactant-producing Pseudomonas and Phytophthora species

    OpenAIRE

    Tran, H.

    2007-01-01

    Fluorescent Pseudomonas bacteria produce a wide variety of antimicrobial metabolites, including soap-like compounds referred to as biosurfactants. The results of this thesis showed that biosurfactant-producing Pseudomonas bacteria are effective in controlling Phytophthora foot rot disease of black pepper in Vietnam and promote root and shoot development of the ‘King of Spices’. Biosurfactant-producing P. fluorescens strain SS101 was also effective in controlling tomato late blight caused by P...

  12. N-Acyl-L-homoserine lactone autoinducers control production of an extracellular lipopeptide biosurfactant required for swarming motility of Serratia liquefaciens MG1

    DEFF Research Database (Denmark)

    Lindum, Peter Wurtz; Anthoni, U; Christophersen, Carsten

    1998-01-01

    A nonswarming Serratia liquefaciens mutant deficient in serrawettin W2 production was constructed by transposon mutagenesis. Sequence homology indicated that insertion had occurred in gene swrA, which encodes a putative peptide synthetase. Expression of swrA is controlled by quorum sensing....

  13. Production optimization of remotely operated gas wells

    Energy Technology Data Exchange (ETDEWEB)

    Juell, Aleksander

    2012-07-01

    From the introduction: The Remote Operations in Oklahoma Intended for Education (ROOKIE) project is a remote field laboratory constructed as a part of this research project. ROOKIE was initiated to provide data in research on production optimization of low productivity gas wells. In addition to this, ROOKIE is used as a teaching tool. Much of the remote operations technology used in the ROOKIE project has been used by the industry for several decades. The first use of remote data acquisition in Oklahoma was in 1989, as described by Luppens [7]. Even though this, for the most part, is old technology, the ROOKIE project is the first remote operations project set up with research and teaching as the main focus. This chapter will discuss the process of establishing the remote field laboratory and the data storage facilities. Results from the project will also be discussed. All testing, instrumentation installation, and modifications to the wells discussed in this chapter was performed by the author. The communication system between the well and NTNU, and the storage database was installed and configured by the author.(Author)

  14. Optimization of medium for the production of subtilisin from Bacillus ...

    African Journals Online (AJOL)

    The effect of yeast extract, casein, peptone and sodium chloride on subtilisin production was studied and were optimized using Box Behnken Design. The optimal growth conditions for B. subtilis MTCC 441 were found to 37oC, and pH 7.5. The optimal media composition for subtilisin production was found to be yeast extract ...

  15. Machine Learning meets Mathematical Optimization to predict the optimal production of offshore wind parks

    DEFF Research Database (Denmark)

    Fischetti, Martina; Fraccaro, Marco

    2018-01-01

    In this paper we propose a combination of Mathematical Optimization and Machine Learning to estimate the value of optimized solutions. In particular, we investigate if a machine, trained on a large number of optimized solutions, could accurately estimate the value of the optimized solution for new...... in production between optimized/non optimized solutions, it is not trivial to understand the potential value of a new site without running a complete optimization. This could be too time consuming if a lot of sites need to be evaluated, therefore we propose to use Machine Learning to quickly estimate...... the potential of new sites (i.e., to estimate the optimized production of a site without explicitly running the optimization). To do so, we trained and tested different Machine Learning models on a dataset of 3000+ optimized layouts found by the optimizer. Thanks to the close collaboration with a leading...

  16. OPTIMIZATION OF PROMOTION EXPENSES USING A PRODUCTION FUNCTION

    Directory of Open Access Journals (Sweden)

    Gleb V. Kamenskiy

    2013-01-01

    Full Text Available This article describes an opportunity of application of the production function for optimization of promotion expenses. Second part of the article contains a data of a real enterprise to calculate an optimal advertising budget.

  17. Antibiotic and biosurfactant properties of cyclic lipopeptides produced by fluorescent Pseudomonas spp. from the sugar beet rhizosphere

    DEFF Research Database (Denmark)

    Nielsen, T H; Sørensen, D; Tobiasen, C

    2002-01-01

    Cyclic lipopeptides (CLPs) with antibiotic and biosurfactant properties are produced by a number of soil bacteria, including fluorescent Pseudomonas spp. To provide new and efficient strains for the biological control of root-pathogenic fungi in agricultural crops, we isolated approximately 600...... in the peptide moiety. Production of specific CLPs could be affiliated with Pseudomonas fluorescens strain groups belonging to biotype I, V, or VI. In vitro analysis using both purified CLPs and whole-cell P. fluorescens preparations demonstrated that all CLPs exhibited strong biosurfactant properties...

  18. Optimal control of raw timber production processes

    Science.gov (United States)

    Ivan Kolenka

    1978-01-01

    This paper demonstrates the possibility of optimal planning and control of timber harvesting activ-ities with mathematical optimization models. The separate phases of timber harvesting are represented by coordinated models which can be used to select the optimal decision for the execution of any given phase. The models form a system whose components are connected and...

  19. Medium optimization for protopectinase production by batch culture of

    African Journals Online (AJOL)

    Medium optimization for protopectinase production by batch culture of. C Fan, Z Liu, L Yao. Abstract. Optimization of medium compositions for protopectinase production by Aspergillus terreus in submerged culture was carried out. The medium components having significant effect on protopectinase production were reported ...

  20. Biosurfactants as green stabilizers for the biological synthesis of nanoparticles.

    Science.gov (United States)

    Kiran, G Seghal; Selvin, Joseph; Manilal, Aseer; Sujith, S

    2011-12-01

    Taking into consideration the needs of greener bioprocesses and novel enhancers for synthesis using microbial processes, biosurfactants, and/or biosurfactant producing microbes are emerging as an alternate source for the rapid synthesis of nanoparticles. A microemulsion technique using an oil-water-surfactant mixture was shown to be a promising approach for nanoparticle synthesis. Biosurfactants are natural surfactants derived from microbial origin composed mostly of sugar and fatty acid moieties, they have higher biodegradability, lower toxicity, and excellent biological activities. The biosurfactant mediated process and microbial synthesis of nanoparticles are now emerging as clean, nontoxic, and environmentally acceptable "green chemistry" procedures. The biosurfactant-mediated synthesis is superior to the methods of bacterial- or fungal-mediated nanoparticle synthesis, since biosurfactants reduce the formation of aggregates due to the electrostatic forces of attraction and facilitate a uniform morphology of the nanoparticles. In this review, we highlight the biosurfactant mediated synthesis of nanoparticles with relevant details including a greener bioprocess, sources of biosurfactants, and biological synthesized nanoparticles based on the available literature and laboratory findings.

  1. Carbohydrate-based renewable biosurfactants: Rhamnolipids, sophorolipids, and novel liamocins

    Science.gov (United States)

    High-yield, glycolipid-based biosurfactants are of increasing interest for use in environmentally benign cleaning or emulsifying agents. We have developed a MALDI-TOF/MS screen for the rapid analysis of several types of biosurfactants, including various acylated rhamnolipids in Pseudomonas extracts...

  2. Interactions between biosurfactant-producing Pseudomonas and Phytophthora species

    NARCIS (Netherlands)

    Tran, H.

    2007-01-01

    Fluorescent Pseudomonas bacteria produce a wide variety of antimicrobial metabolites, including soap-like compounds referred to as biosurfactants. The results of this thesis showed that biosurfactant-producing Pseudomonas bacteria are effective in controlling Phytophthora foot rot

  3. Optimization of enantioselective production of chiral epichlorohydrin ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-10-19

    Oct 19, 2009 ... Among the biocatalytic routes, kinetic resolution of racemic epo- xides via an ... Experimental design and optimization by response surface methodology ..... Optimization of acid hydrolysis from the hemicellulosic fraction of eucalyptus ... Design, synthesis and biological activity of anti-angiogenic hypoxic.

  4. Optimization of extracellular catalase production from Aspergillus ...

    African Journals Online (AJOL)

    aghomotsegin

    extracellular catalase produced by the strain in the optimized medium was about four times higher than ... celial and unicellular fungi in synthetic media (Kurakov et .... covering the appropriate range and the broad calibration kit ... This optimization allowed us to define new cultural con- ..... Ann. New York Academy Sci.

  5. Medium optimization for endochitinase production by recombinant ...

    African Journals Online (AJOL)

    The optimal concentrations of three factors were investigated by the response surface methodology using Box- Behnken design. The optimal medium components obtained for achieving the maximum activity of the endochitinase were as follows: Yeast extract 24.36 g/l, tryptone 20 g/l, YNB 5.0 g/l, potassium phosphate 100 ...

  6. Potential applications of biosurfactant rhamnolipids in agriculture and biomedicine.

    Science.gov (United States)

    Chen, Jianwei; Wu, Qihao; Hua, Yi; Chen, Jun; Zhang, Huawei; Wang, Hong

    2017-12-01

    Rhamnolipids have recently emerged as promising bioactive molecules due to their novel structures, diverse and versatile biological functions, lower toxicity, higher biodegradability, as well as production from renewable resources. The advantages of rhamnolipids make them attractive targets for research in a wide variety of applications. Especially rhamnolipids are likely to possess potential applications of the future in areas such as biomedicine, therapeutics, and agriculture. The purpose of this mini review is to provide a comprehensive prospective of biosurfactant rhamnolipids as potential antimicrobials, immune modulators, and virulence factors, and anticancer agents in the field of biomedicine and agriculture that may meet the ever-increasing future pharmacological treatment and food safety needs in human health.

  7. Synthesis of biosurfactants and their advantages to microorganisms and mankind.

    Science.gov (United States)

    Cameotra, Swaranjit Singh; Makkar, Randhir S; Kaur, Jasminder; Mehta, S K

    2010-01-01

    Biosurfactants are surface-active compounds synthesized by a wide variety of microorganisms. They are molecules that have both hydrophobic and hydrophilic domains and are capable of lowering the surface tension and the interfacial tension of the growth medium. Biosurfactants possess different chemical structures--lipopeptides, glycolipids, neutral lipids and fatty acids. They are nontoxic biomolecules that are biodegradable. Biosurfactants also exhibit strong emulsification of hydrophobic compounds and form stable emulsions. The low water solubility of these hydrophobic compounds limits their availability to microorganisms, which is a potential problem for bioremediation of contaminated sites. Microbially produced surfactants enhance the bioavailability of these hydrophobic compounds for bioremediation. Therefore, biosurfactant-enhanced solubility of pollutants has potential applications in bioremediation. Not only are the biosurfactants useful in a variety of industrial processes, they are also of vital importance to the microbes in adhesion, emulsification, bioavailability, desorption and defense strategy. These interesting facts are discussed in this chapter.

  8. Microbial biosurfactants: challenges and opportunities for future exploitation.

    Science.gov (United States)

    Marchant, Roger; Banat, Ibrahim M

    2012-11-01

    The drive for industrial sustainability has pushed biosurfactants to the top of the agenda of many companies. Biosurfactants offer the possibility of replacing chemical surfactants, produced from nonrenewable resources, with alternatives produced from cheap renewable feedstocks. Biosurfactants are also attractive because they are less damaging to the environment yet are robust enough for industrial use. The most promising biosurfactants at the present time are the glycolipids, sophorolipids produced by Candida yeasts, mannosylerythritol lipids (MELs) produced by Pseudozyma yeasts, and rhamnolipids produced by Pseudomonas. Despite the current enthusiasm for these compounds several residual problems remain. This review highlights remaining problems and indicates the prospects for imminent commercial exploitation of a new generation of microbial biosurfactants. Copyright © 2012 Elsevier Ltd. All rights reserved.

  9. Effect of rhamnolipid biosurfactant on solubilization of polycyclic aromatic hydrocarbons.

    Science.gov (United States)

    Li, Shudong; Pi, Yongrui; Bao, Mutai; Zhang, Cong; Zhao, Dongwei; Li, Yiming; Sun, Peiyan; Lu, Jinren

    2015-12-15

    Rhamnolipid biosurfactant-producing bacteria, Bacillus Lz-2, was isolated from oil polluted water collected from Dongying Shengli oilfield, China. The factors that influence PAH solubilization such as biosurfactant concentration, pH, ionic strength and temperature were discussed. The results showed that the solubilities of naphthalene, phenanthrene and pyrene increased linearly with the rise of rhamnolipid biosurfactant dose above the biosurfactant critical micelle concentration (CMC). Furthermore, the molar solubilization ratio (MSR) values decreased in the following order: naphthalene>phenanthrene>pyrene. However, the solubility percentage increased and followed the opposite order: pyrene>phenanthrene>naphthalene. The solubilities of PAHs in rhamnolipid biosurfactant solution increased with the rise of pH and ionic strength, and reached the maximum values under the conditions of pH11 and NaCl concentration 8 g · L(-1). The solubility of phenanthrene and pyrene increased with the rise of temperature. Copyright © 2015 Elsevier Ltd. All rights reserved.

  10. Production and optimization of ligninolytic enzymes by white rot ...

    African Journals Online (AJOL)

    Production and optimization of ligninolytic enzymes by white rot fungus Schizophyllum ... size and nutritional factors (carbon and nitrogen ratio, mediators and metal ions). ... scale production of these enzymes for diverse industrial applications.

  11. Optimization of alkaline protease production and its fibrinolytic ...

    African Journals Online (AJOL)

    Optimization of alkaline protease production and its fibrinolytic activity from the ... nitrogen sources and sodium chloride concentration for protease production by the ... exploited to assist in protein degradation in various industrial processes.

  12. Optimization of extracellular polysaccharide production in ...

    African Journals Online (AJOL)

    sunny

    2014-11-26

    Nov 26, 2014 ... textiles, detergents, adhesives, cosmetics, pharmaceu- ticals, food additives ... Isolation and identification of EPS producing halophilic bacteria ..... The present study lead to the optimization of key culture conditions with CCD ...

  13. Optimal production planning for PCB assembly

    CERN Document Server

    Ho, William

    2006-01-01

    Focuses on the optimization of the Printed circuit board (PCB) assembly lines' efficiency. This book integrates the component sequencing and the feeder arrangement problems together for the pick-and-place machine and the chip shooter machines.

  14. Optimization of soybean \\'dawadawa\\' production, using response ...

    African Journals Online (AJOL)

    dawadawa\\'. Response surface experimental design with 53 combinations in replicate was used to optimize the six processing variables. This was used to determine the optimum combinations of five processing variables that will produce the most ...

  15. Optimization of Citric Acid Production through Manipulation of ...

    African Journals Online (AJOL)

    An Aspergillus niger isolate was screened for citric acid production from glucose and the cultural conditions were manipulated for optimum citric acid production. Optimization studies improved citric acid yield by 13.34% from 12.81 g/l obtained during the screening test to 14.52 g/l obtained at the end of the optimization ...

  16. Biosurfactants produced by Scheffersomyces stipitis cultured in sugarcane bagasse hydrolysate as new green larvicides for the control of Aedes aegypti, a vector of neglected tropical diseases.

    Science.gov (United States)

    Franco Marcelino, Paulo Ricardo; da Silva, Vinícius Luiz; Rodrigues Philippini, Rafael; Von Zuben, Cláudio José; Contiero, Jonas; Dos Santos, Júlio César; da Silva, Silvio Silvério

    2017-01-01

    Biosurfactants are microbial metabolites with possible applications in various industrial sectors that are considered ecofriendly molecules. In recent years, some studies identified these compounds as alternatives for the elimination of vectors of tropical diseases, such as Aedes aegypti. The major bottlenecks of biosurfactant industrial production have been the use of conventional raw materials that increase production costs as well as opportunistic or pathogenic bacteria, which restrict the application of these biomolecules. The present study shows the potential of hemicellulosic sugarcane bagasse hydrolysate as a raw material for the production of a crystalline glycolipidic BS by Scheffersomyces stipitis NRRL Y-7124, which resulted in an emulsifying index (EI24) of 70 ± 3.4% and a superficial tension of 52 ± 2.9 mN.m-1. Additionally, a possible new application of these compounds as biolarvicides, mainly against A. aegypti, was evaluated. At a concentration of 800 mg.L-1, the produced biosurfactant caused destruction to the larval exoskeletons 12 h after application and presented an letal concentration (LC50) of 660 mg.L-1. Thus, a new alternative for biosurfactant production using vegetal biomass as raw material within the concept of biorefineries was proposed, and the potential of the crystalline glycolipidic biosurfactant in larvicidal formulations against neglected tropical disease vectors was demonstrated.

  17. Biosurfactants produced by Scheffersomyces stipitis cultured in sugarcane bagasse hydrolysate as new green larvicides for the control of Aedes aegypti, a vector of neglected tropical diseases.

    Directory of Open Access Journals (Sweden)

    Paulo Ricardo Franco Marcelino

    Full Text Available Biosurfactants are microbial metabolites with possible applications in various industrial sectors that are considered ecofriendly molecules. In recent years, some studies identified these compounds as alternatives for the elimination of vectors of tropical diseases, such as Aedes aegypti. The major bottlenecks of biosurfactant industrial production have been the use of conventional raw materials that increase production costs as well as opportunistic or pathogenic bacteria, which restrict the application of these biomolecules. The present study shows the potential of hemicellulosic sugarcane bagasse hydrolysate as a raw material for the production of a crystalline glycolipidic BS by Scheffersomyces stipitis NRRL Y-7124, which resulted in an emulsifying index (EI24 of 70 ± 3.4% and a superficial tension of 52 ± 2.9 mN.m-1. Additionally, a possible new application of these compounds as biolarvicides, mainly against A. aegypti, was evaluated. At a concentration of 800 mg.L-1, the produced biosurfactant caused destruction to the larval exoskeletons 12 h after application and presented an letal concentration (LC50 of 660 mg.L-1. Thus, a new alternative for biosurfactant production using vegetal biomass as raw material within the concept of biorefineries was proposed, and the potential of the crystalline glycolipidic biosurfactant in larvicidal formulations against neglected tropical disease vectors was demonstrated.

  18. Combination of bioleaching by gross bacterial biosurfactants and flocculation: A potential remediation for the heavy metal contaminated soils.

    Science.gov (United States)

    Yang, Zhihui; Shi, Wei; Yang, Weichun; Liang, Lifen; Yao, Wenbin; Chai, Liyuan; Gao, Shikang; Liao, Qi

    2018-09-01

    Combining bioleaching by the gross biosurfactants of Burkholderia sp. Z-90 and flocculation by poly aluminium chloride (PAC) was proposed to develop a potential environment-friendly and cost-effective technique to remediate the severely contaminated soils by heavy metals. The factors affecting soil bioleaching by the gross biosurfactants of Burkholderia sp. Z-90 were optimized. The results showed the optimal removing efficiencies of Zn, Pb, Mn, Cd, Cu, and As by the Burkholderia sp. Z-90 leachate were 44.0, 32.5, 52.2, 37.7, 24.1 and 31.6%, respectively at soil liquid ratio of 1:20 (w/v) for 5 d, which were more efficient than that by 0.1% of rhamnolipid. The amounts of the bioleached heavy metals by the Burkholderia sp. Z-90 leachate were higher than that by other biosurfactants in the previous studies, although the removal efficiencies of the metals by the leachate were relatively lower. It was suggested that more heavy metals caused more competitive to chelate with function groups of the gross biosurfactants and the metal removal efficiencies by biosurfactants in natural soils were lower than in the artificially contaminated soils. Moreover, the Burkholderia sp. Z-90 leachate facilitated the metals to be transformed to the easily migrating speciation fractions. Additional, the results showed that PAC was efficient in the following flocculation to remove heavy metals in the waste bio-leachates. Our study will provide support for developing a bioleaching technique model to remediate the soils extremely contaminated by heavy metals. Copyright © 2018 Elsevier Ltd. All rights reserved.

  19. Utilization of Crude Glycerol as a Substrate for the Production of Rhamnolipid by Pseudomonas aeruginosa

    OpenAIRE

    Eraqi, Walaa A.; Yassin, Aymen S.; Ali, Amal E.; Amin, Magdy A.

    2016-01-01

    Biosurfactants are produced by bacteria or yeast utilizing different substrates as sugars, glycerol, or oils. They have important applications in the detergent, oil, and pharmaceutical industries. Glycerol is the product of biodiesel industry and the existing glycerol market cannot accommodate the excess amounts generated; consequently, new markets for refined glycerol need to be developed. The aim of present work is to optimize the production of microbial rhamnolipid using waste glycerol. We...

  20. Trehalose lipid biosurfactants produced by the actinomycetes Tsukamurella spumae and T. pseudospumae.

    Science.gov (United States)

    Kügler, Johannes H; Muhle-Goll, Claudia; Kühl, Boris; Kraft, Axel; Heinzler, Raphael; Kirschhöfer, Frank; Henkel, Marius; Wray, Victor; Luy, Burkhard; Brenner-Weiss, Gerald; Lang, Siegmund; Syldatk, Christoph; Hausmann, Rudolf

    2014-11-01

    Actinomycetales are known to produce various secondary metabolites including products with surface-active and emulsifying properties known as biosurfactants. In this study, the nonpathogenic actinomycetes Tsukamurella spumae and Tsukamurella pseudospumae are described as producers of extracellular trehalose lipid biosurfactants when grown on sunflower oil or its main component glyceryltrioleate. Crude extracts of the trehalose lipids were purified using silica gel chromatography. The structure of the two trehalose lipid components (TL A and TL B) was elucidated using a combination of matrix-assisted laser desorption/ionization time-of-flight/time-of-flight/tandem mass spectroscopy (MALDI-ToF-ToF/MS/MS) and multidimensional NMR experiments. The biosurfactants were identified as 1-α-glucopyranosyl-1-α-glucopyranosid carrying two acyl chains varying of C4 to C6 and C16 to C18 at the 2' and 3' carbon atom of one sugar unit. The trehalose lipids produced demonstrate surface-active behavior and emulsifying capacity. Classified as risk group 1 organisms, T. spumae and T. pseudospumae hold potential for the production of environmentally friendly surfactants.

  1. Selective cultures for the isolation of biosurfactant producing bacteria: comparison of different combinations of environmental inocula and hydrophobic carbon sources.

    Science.gov (United States)

    Domingues, Patrícia M; Louvado, António; Oliveira, Vanessa; Coelho, Francisco J C R; Almeida, Adelaide; Gomes, Newton C M; Cunha, Angela

    2013-01-01

    The potential of estuarine microniches as reservoirs of biosurfactant-producing bacteria was evaluated by testing different combinations of inocula and hydrophobic carbon sources. Selective cultures using diesel, petroleum, or paraffin as hydrophobic carbon sources were prepared and inoculated with water from the surface microlayer, bulk sediments, and sediment of the rhizosphere of Halimione portulacoides. These inocula were compared regarding the frequency of biosurfactant-producing strains among selected isolates. The community structure of the selective cultures was profiled using denaturing gradient gel electrophoresis (DGGE) of the 16S rRNA gene fragments at the end of the incubation. The DGGE profiles corresponding to the communities established in selective cultures at the end of the incubation revealed that communities were different in terms of structural diversity. The highest diversity was observed in the selective cultures containing paraffin (H (') = 2.5). Isolates were obtained from the selective cultures (66) and tested for biosurfactant production by the atomized oil assay. Biosurfactant production was detected in 17 isolates identified as Microbacterium, Pseudomonas, Rhodococcus, and Serratia. The combination of estuarine surface microlayer (SML) water as inoculum and diesel as carbon source seems promising for the isolation of surfactant-producing bacteria. Supplemental materials are available for this article. Go to the publisher's online edition of Preparative Biochemistry and Biotechnology to view the supplemental file.

  2. Isolation and characterization of biosurfactant-producing Alcanivorax strains: hydrocarbon accession strategies and alkane hydroxylase gene analysis.

    Science.gov (United States)

    Olivera, Nelda L; Nievas, Marina L; Lozada, Mariana; Del Prado, Guillermo; Dionisi, Hebe M; Siñeriz, Faustino

    2009-01-01

    Biosurfactant-producing bacteria belonging to the genera Alcanivorax, Cobetia and Halomonas were isolated from marine sediments with a history of hydrocarbon exposure (Aristizábal and Gravina Peninsulas, Argentina). Two Alcanivorax isolates were found to form naturally occurring consortia with strains closely related to Pseudomonas putida and Microbacterium esteraromaticum. Alkane hydroxylase gene analysis in these two Alcanivorax strains resulted in the identification of two novel alkB genes, showing 86% and 60% deduced amino acid sequence identity with those of Alcanivorax sp. A-11-3 and Alcanivorax dieselolei P40, respectively. In addition, a gene homologous to alkB2 from Alcanivorax borkumensis was present in one of the strains. The consortium formed by this strain, Alcanivorax sp. PA2 (98.9% 16S rRNA gene sequence identity with A. borkumensis SK2(T)) and P. putida PA1 was characterized in detail. These strains form cell aggregates when growing as mixed culture, though only PA2 was responsible for biosurfactant activity. During exponential growth phase of PA2, cells showed high hydrophobicity and adherence to hydrocarbon droplets. Biosurfactant production was only detectable at late growth and stationary phases, suggesting that it is not involved in initiating oil degradation and that direct interfacial adhesion is the main hydrocarbon accession mode of PA2. This strain could be useful for biotechnological applications due to its biosurfactant production, catabolic and aggregation properties.

  3. Optimization of extracellular catalase production from Aspergillus ...

    African Journals Online (AJOL)

    The studies of the effect of each variable and the establishment of a correlation between the response of enzyme activity and variables revealed that the link is a multiple linear regression form. The optimization was carried out through a simplex algorithm. The amount of extracellular catalase produced by the strain in the ...

  4. Codon optimizing for increased membrane protein production

    DEFF Research Database (Denmark)

    Mirzadeh, K.; Toddo, S.; Nørholm, Morten

    2016-01-01

    . As demonstrated with two membrane-embedded transporters in Escherichia coli, the method was more effective than optimizing the entire coding sequence. The method we present is PCR based and requires three simple steps: (1) the design of two PCR primers, one of which is degenerate; (2) the amplification...

  5. NMPC for Oil Reservoir Production Optimization

    DEFF Research Database (Denmark)

    Völcker, Carsten; Jørgensen, John Bagterp; Thomsen, Per Grove

    2011-01-01

    this problem numerically using a single shooting sequential quadratic programming (SQP) based optimization method. Explicit singly diagonally implicit Runge-Kutta (ESDIRK) methods are used for integration of the stiff system of differential equations describing the two-phase flow, and the adjoint method...

  6. Screening of biosurfactants from cloud microorganisms

    Science.gov (United States)

    Sancelme, Martine; Canet, Isabelle; Traikia, Mounir; Uhliarikova, Yveta; Capek, Peter; Matulova, Maria; Delort, Anne-Marie; Amato, Pierre

    2015-04-01

    The formation of cloud droplets from aerosol particles in the atmosphere is still not well understood and a main source of uncertainties in the climate budget today. One of the principal parameters in these processes is the surface tension of atmospheric particles, which can be strongly affected by trace compounds called surfactants. Within a project devoted to bring information on atmospheric surfactants and their effects on cloud droplet formation, we focused on surfactants produced by microorganisms present in atmospheric waters. From our unique collection of microorganisms, isolated from cloud water collected at the Puy-de-Dôme (France),1 we undertook a screening of this bank for biosurfactant producers. After extraction of the supernatants of the pure cultures, surface tension of crude extracts was determined by the hanging drop technique. Results showed that a wide variety of microorganisms are able to produce biosurfactants, some of them exhibiting strong surfactant properties as the resulting tension surface decreases to values less then 35 mN.m-1. Preliminary analytical characterization of biosurfactants, obtained after isolation from overproducing cultures of Rhodococcus sp. and Pseudomonas sp., allowed us to identify them as belonging to two main classes, namely glycolipids and glycopeptides. 1. Vaïtilingom, M.; Attard, E.; Gaiani, N.; Sancelme, M.; Deguillaume, L.; Flossmann, A. I.; Amato, P.; Delort, A. M. Long-term features of cloud microbiology at the puy de Dôme (France). Atmos. Environ. 2012, 56, 88-100. Acknowledgements: This work is supported by the French-USA ANR SONATA program and the French-Slovakia programs Stefanik and CNRS exchange.

  7. Optimization of alkaline protease production from Pseudomonas ...

    African Journals Online (AJOL)

    PRECIOUS

    2009-12-15

    Dec 15, 2009 ... protease production was 37°C at pH 9, with 2% inoculum in the medium for 24 h. .... Positive. Catalase test. Positive ... The enzyme activity gradually decreases from ... Effect of temperature on protease production by Pseudomonas fluorescens. 0 .... between RNA polymerase and upstream promotes DNA.

  8. Asset management -- Integrated software optimizes production performance

    International Nuclear Information System (INIS)

    Polczer, S.

    1998-01-01

    Developments in data collection and retrieval systems to allow timely cost analysis, financial reporting and production management are discussed. One of the most important new OLAP (on-line analytical processing) products is Energy Warehouse which gathers field information from various sources, allows advanced searches, and generates reports previously unavailable in other conventional financial accounting systems. Another OLAP-based system, the Canadian Upstream Energy System (CUES), was developed by the Oracle Corporation and the Calgary-based Applied Terravision Systems (ATS) Inc. CUES combines Oracle's universal data server software development tools with ATS's upstream financial, land, geotechnical and production applications. ATS also developed a product called IDPMARS (Integrated Daily Production Management Accounting Reporting System). It interfaces with CUES to link working interests, government royalties, administration, facility charges, lifting costs, transportation tooling, and customers by integrating field data collection systems with financial accounting

  9. Asset management -- Integrated software optimizes production performance

    Energy Technology Data Exchange (ETDEWEB)

    Polczer, S.

    1998-10-01

    Developments in data collection and retrieval systems to allow timely cost analysis, financial reporting and production management are discussed. One of the most important new OLAP (on-line analytical processing) products is Energy Warehouse which gathers field information from various sources, allows advanced searches, and generates reports previously unavailable in other conventional financial accounting systems. Another OLAP-based system, the Canadian Upstream Energy System (CUES), was developed by the Oracle Corporation and the Calgary-based Applied Terravision Systems (ATS) Inc. CUES combines Oracle`s universal data server software development tools with ATS`s upstream financial, land, geotechnical and production applications. ATS also developed a product called IDPMARS (Integrated Daily Production Management Accounting Reporting System). It interfaces with CUES to link working interests, government royalties, administration, facility charges, lifting costs, transportation tooling, and customers by integrating field data collection systems with financial accounting.

  10. An Optimization Study on Syngas Production and Economic Evaluation

    Directory of Open Access Journals (Sweden)

    Qasim Faraz

    2016-01-01

    Full Text Available Syngas production in Gas-to-liquid (GTL process is focused in past by several researchers to increase the production with minimal capital and operating costs. In this study, syngas production process is simulated and optimized to increase its production and the economic analysis is studied for the proposed optimized process. Aspen HYSYS v8.4 is used for all process simulation work in this article. A new configuration is rigorously simulated while using auto-thermal reforming. Results exhibit a tremendous rise in production of syngas.

  11. Supporting data for identification of biosurfactant-producing bacteria isolated from agro-food industrial effluent

    Science.gov (United States)

    Fulazzaky, Mohamad Ali; Abdullah, Shakila; Salim, Mohd Razman

    2016-01-01

    The goal of this study was to identify the biosurfactant-producing bacteria isolated from agro-food industrial effluet. The identification of the potential bacterial strain using a polymerase chain reaction of the 16S rRNA gene analysis was closely related to Serratia marcescens with its recorded strain of SA30 “Fundamentals of mass transfer and kinetics for biosorption of oil and grease from agro-food industrial effluent by Serratia marcescens SA30” (Fulazzaky et al., 2015) [1]; however, many biochemical tests have not been published yet. The biochemical tests of biosurfactant production, haemolytic assay and cell surface hydrophobicity were performed to investigate the beneficial strain of biosurfactant-producing bacteria. Here we do share data collected from the biochemical tests to get a better understanding of the use of Serratia marcescens SA30 to degrade oil, which contributes the technical features of strengthening the biological treatment of oil-contaminated wastewater in tropical environments. PMID:27077083

  12. Supporting data for identification of biosurfactant-producing bacteria isolated from agro-food industrial effluent

    Directory of Open Access Journals (Sweden)

    Mohamad Ali Fulazzaky

    2016-06-01

    Full Text Available The goal of this study was to identify the biosurfactant-producing bacteria isolated from agro-food industrial effluet. The identification of the potential bacterial strain using a polymerase chain reaction of the 16S rRNA gene analysis was closely related to Serratia marcescens with its recorded strain of SA30 “Fundamentals of mass transfer and kinetics for biosorption of oil and grease from agro-food industrial effluent by Serratia marcescens SA30” (Fulazzaky et al., 2015 [1]; however, many biochemical tests have not been published yet. The biochemical tests of biosurfactant production, haemolytic assay and cell surface hydrophobicity were performed to investigate the beneficial strain of biosurfactant-producing bacteria. Here we do share data collected from the biochemical tests to get a better understanding of the use of Serratia marcescens SA30 to degrade oil, which contributes the technical features of strengthening the biological treatment of oil-contaminated wastewater in tropical environments.

  13. Optimizing the order processing of customized products using product configuration

    DEFF Research Database (Denmark)

    Hvam, Lars; Bonev, Martin; Denkena, B.

    2011-01-01

    . Product configuration based on integrated modular product structure and product family architecture has been recognized as an effective means for implementing mass customization. In order to evaluate the effects of product configuration on order processing, a study has been conducted by the Department...... and its benefits for the order processing have been evaluated....

  14. Evolutionary optimization of production materials workflow processes

    DEFF Research Database (Denmark)

    Herbert, Luke Thomas; Hansen, Zaza Nadja Lee; Jacobsen, Peter

    2014-01-01

    We present an evolutionary optimisation technique for stochastic production processes, which is able to find improved production materials workflow processes with respect to arbitrary combinations of numerical quantities associated with the production process. Working from a core fragment...... of the BPMN language, we employ an evolutionary algorithm where stochastic model checking is used as a fitness function to determine the degree of improvement of candidate processes derived from the original process through mutation and cross-over operations. We illustrate this technique using a case study...

  15. Isolation and characterization of a novel biosurfactant produced by hydrocarbon-degrading bacterium Alcanivorax dieselolei B-5.

    Science.gov (United States)

    Qiao, N; Shao, Z

    2010-04-01

    Our goal was to identify a novel biosurfactant produced by a marine oil-degrading bacterium. Biosurfactants were produced by Alcanivorax dieselolei strain B-5(T) growing with diesel oil as the sole carbon and energy source. Culture supernatant was first extracted with chloroform/methanol (1:1, v/v), then further purified step by step with a normal phase silica gel column, a Sephadex LH20 gel column and a preparative thin layer plate. The main component was determined to be a lipopeptide; it was chemically characterized with nuclear magnetic resonance, liquid chromatography-quadrupole ion-trap mass spectrometry, amino acid analysis and GC-MS and was found to be a mixture of proline lipids. The monomers of the proline lipids were composed of a proline residue and a fatty acid (C(14:0), C(16:0) or C(18:0)). The critical micelle concentration of the mixed proline lipids was determined to be 40 mg l(-1). Moreover, activity variations in ranges of pH, temperature and salinity were also detected and showed reasonable stability. Alcanivorax dieselolei B-5 produced a novel linear lipoamino biosurfactant, characterized as a proline lipid. A proline lipid was characterized for the first time as a bacterial biosurfactant. This product has potential in both environmental and industrial applications.

  16. Optimization of bioethanol production from simultaneous ...

    African Journals Online (AJOL)

    ADOWIE PERE

    fermentation of pineapple peels using Saccharomyces cerevisiae ... ABSTRACT: In this study, bioethanol production from the simultaneous ... in turn has resulted in the need to find a source of ... fruit in the world after Banana and Citrus and.

  17. Production, optimization, characterization and antifungal activity of ...

    African Journals Online (AJOL)

    Aspergillus terrus was found to be a good chitinase producer among the five fungi ... The high level of chitinase production was observed in the culture medium ... A. terrus chitinase was investigated against Apergillus niger, Aspergillus oryzae, ...

  18. Enhancing product robustness in reliability-based design optimization

    International Nuclear Information System (INIS)

    Zhuang, Xiaotian; Pan, Rong; Du, Xiaoping

    2015-01-01

    Different types of uncertainties need to be addressed in a product design optimization process. In this paper, the uncertainties in both product design variables and environmental noise variables are considered. The reliability-based design optimization (RBDO) is integrated with robust product design (RPD) to concurrently reduce the production cost and the long-term operation cost, including quality loss, in the process of product design. This problem leads to a multi-objective optimization with probabilistic constraints. In addition, the model uncertainties associated with a surrogate model that is derived from numerical computation methods, such as finite element analysis, is addressed. A hierarchical experimental design approach, augmented by a sequential sampling strategy, is proposed to construct the response surface of product performance function for finding optimal design solutions. The proposed method is demonstrated through an engineering example. - Highlights: • A unifying framework for integrating RBDO and RPD is proposed. • Implicit product performance function is considered. • The design problem is solved by sequential optimization and reliability assessment. • A sequential sampling technique is developed for improving design optimization. • The comparison with traditional RBDO is provided

  19. Constrained optimization via simulation models for new product innovation

    Science.gov (United States)

    Pujowidianto, Nugroho A.

    2017-11-01

    We consider the problem of constrained optimization where the decision makers aim to optimize the primary performance measure while constraining the secondary performance measures. This paper provides a brief overview of stochastically constrained optimization via discrete event simulation. Most review papers tend to be methodology-based. This review attempts to be problem-based as decision makers may have already decided on the problem formulation. We consider constrained optimization models as there are usually constraints on secondary performance measures as trade-off in new product development. It starts by laying out different possible methods and the reasons using constrained optimization via simulation models. It is then followed by the review of different simulation optimization approach to address constrained optimization depending on the number of decision variables, the type of constraints, and the risk preferences of the decision makers in handling uncertainties.

  20. Optimal design of a multi-product biorefinery system

    DEFF Research Database (Denmark)

    Zondervan, E.; Nawaz, Mehboob; de Haan, André B.

    2011-01-01

    In this paper we propose a biorefinery optimization model that can be used to find the optimal processing route for the production of ethanol, butanol, succinic acid and blends of these chemicals with fossil fuel based gasoline. The approach unites transshipment models with a superstructure...

  1. Optimization of fermentation conditions for red pigment production ...

    African Journals Online (AJOL)

    An extracellular pigment-producing ascomycetous filamentous fungi belonging to the genera Penicillium was obtained from soil and its optimal culture conditions investigated. The optimal culture conditions for pigment production were as follows; soluble starch 2% (670 units), peptone (880 units), pH 9.0 (900 units); ...

  2. Optimization of radiation protection of cell maintenance of radiopharmaceutical production

    International Nuclear Information System (INIS)

    Machado, Jessica S.; Gerulis, Eduardo; Todo, Alberto S.; Rodrigues Junior, Orlando

    2011-01-01

    This paper performs a study of maintenance tasks realized in production cells to suggest improvements related to the occupational doses based on the optimization principle of the radioprotection. A data survey has been realized of the doses received by the workers during the maintenance. The average values of effective doses are lower than the limits established in the regulations, however can be optimized

  3. Oil Reservoir Production Optimization using Single Shooting and ESDIRK Methods

    DEFF Research Database (Denmark)

    Capolei, Andrea; Völcker, Carsten; Frydendall, Jan

    2012-01-01

    the injections and oil production such that flow is uniform in a given geological structure. Even in the case of conventional water flooding, feedback based optimal control technologies may enable higher oil recovery than with conventional operational strategies. The optimal control problems that must be solved...

  4. Optimization of alkaline protease production by Streptomyces sp ...

    African Journals Online (AJOL)

    Hacene

    2016-06-29

    Jun 29, 2016 ... The enzyme production media are optimized according to statistical methods while using two plans of ..... Coagulation. -. Nitrate reduction. + .... (i)Fructose: is a simple sugar found naturally in fruits and honey but can be ...

  5. Optimization of fermentation conditions for trehalose production by a ...

    African Journals Online (AJOL)

    user

    2012-02-16

    Feb 16, 2012 ... The culture conditions for the production of trehalose by Rhodotorula sp. strain were optimized. The optimum ... INTRODUCTION. Trehalose is a ..... Water interplay in trehalose ... In: Prescott DM (Ed.), Methods in Cell Biology.

  6. Draft Genome Sequence of a Biosurfactant-Producing Bacillus subtilis UMX-103 Isolated from Hydrocarbon-Contaminated Soil in Terengganu, Malaysia.

    Science.gov (United States)

    Abdelhafiz, Yousri Abdelmutalab; Manaharan, Thamilvaani; BinMohamad, Saharuddin; Merican, Amir Feisal

    2017-07-01

    The draft genome here presents the sequence of Bacillus subtilis UMX-103. The bacterial strain was isolated from hydrocarbon-contaminated soil from Terengganu, Malaysia. The whole genome of the bacterium was sequenced using Illumina HiSeq 2000 sequencing platform. The genome was assembled using de novo approach. The genome size of UMX-103 is 4,234,627 bp with 4399 genes comprising 4301 protein-coding genes and 98 RNA genes. The analysis of assembled genes revealed the presence of 25 genes involved in biosurfactant production, where 14 of the genes are related to biosynthesis and 11 of the genes are in the regulation of biosurfactant productions. This draft genome will provide insights into the genetic bases of its biosurfactant-producing capabilities.

  7. Optimization of biogas production from manure

    DEFF Research Database (Denmark)

    Kaparaju, Prasad Laxmi-Narasimha; Boe, Kanokwan; Buendia, Inmaculada M.

    -scale studies showed that serial digestion with 77/23% volume distribution produced 1.9-6.1% more biogas compared to that obtained during one-step CSTR operation. However, temperature was found to have a strong influence on the methane production and process performance of the second reactor of a serial CSTR......The main objective of the project was to improve biogas production from manures. This objective was addressed by investigating 1) the effect of different reactor configurations, 2) operational procedures, aiming to selectively retain/return degradable material in the reactor and 3) different...... process at 90/10, 80/20, 70/30, 50/50 or 30/70% volume distribution could produce 11-17.8% more biogas compared to single CSTR process under similar operating conditions. The increased biogas production was mainly from the second reactor of the serial process, which accounted for 16-18% of the total...

  8. Gradient-based methods for production optimization of oil reservoirs

    Energy Technology Data Exchange (ETDEWEB)

    Suwartadi, Eka

    2012-07-01

    Production optimization for water flooding in the secondary phase of oil recovery is the main topic in this thesis. The emphasis has been on numerical optimization algorithms, tested on case examples using simple hypothetical oil reservoirs. Gradientbased optimization, which utilizes adjoint-based gradient computation, is used to solve the optimization problems. The first contribution of this thesis is to address output constraint problems. These kinds of constraints are natural in production optimization. Limiting total water production and water cut at producer wells are examples of such constraints. To maintain the feasibility of an optimization solution, a Lagrangian barrier method is proposed to handle the output constraints. This method incorporates the output constraints into the objective function, thus avoiding additional computations for the constraints gradient (Jacobian) which may be detrimental to the efficiency of the adjoint method. The second contribution is the study of the use of second-order adjoint-gradient information for production optimization. In order to speedup convergence rate in the optimization, one usually uses quasi-Newton approaches such as BFGS and SR1 methods. These methods compute an approximation of the inverse of the Hessian matrix given the first-order gradient from the adjoint method. The methods may not give significant speedup if the Hessian is ill-conditioned. We have developed and implemented the Hessian matrix computation using the adjoint method. Due to high computational cost of the Newton method itself, we instead compute the Hessian-timesvector product which is used in a conjugate gradient algorithm. Finally, the last contribution of this thesis is on surrogate optimization for water flooding in the presence of the output constraints. Two kinds of model order reduction techniques are applied to build surrogate models. These are proper orthogonal decomposition (POD) and the discrete empirical interpolation method (DEIM

  9. PROPOSAL OF SPATIAL OPTIMIZATION OF PRODUCTION PROCESS IN PROCESS DESIGNER

    Directory of Open Access Journals (Sweden)

    Peter Malega

    2015-03-01

    Full Text Available This contribution is focused on optimizing the use of space in the production process using software Process Designer. The aim of this contribution is to suggest possible improvements to the existing layout of the selected production process. Production process was analysed in terms of inputs, outputs and course of actions. Nowadays there are many software solutions aimed at optimizing the use of space. One of these software products is the Process Designer, which belongs to the product line Tecnomatix. This software is primarily aimed at production planning. With Process Designer is possible to design the layout of production and subsequently to analyse the production or to change according to the current needs of the company.

  10. Optimization benefits analysis in production process of fabrication components

    Science.gov (United States)

    Prasetyani, R.; Rafsanjani, A. Y.; Rimantho, D.

    2017-12-01

    The determination of an optimal number of product combinations is important. The main problem at part and service department in PT. United Tractors Pandu Engineering (shortened to PT.UTPE) Is the optimization of the combination of fabrication component products (known as Liner Plate) which influence to the profit that will be obtained by the company. Liner Plate is a fabrication component that serves as a protector of core structure for heavy duty attachment, such as HD Vessel, HD Bucket, HD Shovel, and HD Blade. The graph of liner plate sales from January to December 2016 has fluctuated and there is no direct conclusion about the optimization of production of such fabrication components. The optimal product combination can be achieved by calculating and plotting the amount of production output and input appropriately. The method that used in this study is linear programming methods with primal, dual, and sensitivity analysis using QM software for Windows to obtain optimal fabrication components. In the optimal combination of components, PT. UTPE provide the profit increase of Rp. 105,285,000.00 for a total of Rp. 3,046,525,000.00 per month and the production of a total combination of 71 units per unit variance per month.

  11. Optimal sulphuric acid production using Acidithiobacillus caldus ...

    African Journals Online (AJOL)

    AFRICAN JOURNALS ONLINE (AJOL) · Journals · Advanced Search · USING ... oxygen uptake rate of 1.35 g/L.day (OUR), 52% sulphur conversion at a rate of 0.83 ... achieving a sulphuric acid production rate of 2.76 g/L.day (dP/dt), while the ...

  12. Optimization of biomass and dihydroorotase (DHOase) production ...

    African Journals Online (AJOL)

    Growth conditions which maintains DHOase overproduction by Saccharomyces cerevisiae MNJ3 (pMNJ1) and allow sufficient biomass production to ensure DHoase's purification were investigated. We used as basal medium the Yeast Carbon Base (YCB; Difco), especially designed for studies of nitrogen metabolism in ...

  13. OPTIMAL MANAGEMENT FOR WATERS FOR THE PRODUCTION ...

    African Journals Online (AJOL)

    30 juin 2010 ... demande, car d'une part la production est proportionnelle au ... [2] Fosso O B., Belsnes M M. International conference on power system technology. ... [7] Ray W H., Soleman M A. Eleventh joint automatic conference of the ...

  14. Application of the biosurfactants produced by Bacillus spp. (SH 20 ...

    African Journals Online (AJOL)

    Application of the biosurfactants produced by Bacillus spp. (SH 20 and SH 26) and P. aeruginosa SH 29 isolated from the rhizosphere soil of an Egyptian salt marsh plant for the cleaning of oil - contaminataed vessels and enhancing the biodegradat.

  15. Biosurfactant-enhanced bioremediation of polycyclic aromatic hydrocarbons

    Energy Technology Data Exchange (ETDEWEB)

    Cameotra, S.S.; Bollag, J.M. [Penn State University, University Park, PA (USA). Soil Biochemical Lab.

    2003-07-01

    Biosurfactants are surface-active compounds synthesized by it wide variety of micro-organisms. They are molecules that have both hydrophobic and hydrophilic domains and are capable of lowering the surface tension and the interfacial tension of the growth medium. Biosurfactants possess different chemical structures - lipopeptides, glycolipids, neutral lipids, and fatty acids. They are nontoxic biomolecules that are biodegradable. Biosurfactants also exhibit strong emulsification of hydrophobic compounds and form stable emulsions. Polycyclic aromatic hydrocarbons (PAHs) can be toxic, mutagenic, and carcinogenic compounds that pollute the environment. They are released to the environment its a result of spillage of oil and byproducts of coal treatment processes. The low water solubility of PAHs limits their availability to microorganisms, which is a potential problem for bioremediation of PAH-contaminated sites. Microbially produced surfactants enhance the bioavailability of these hydrophobic compounds for bioremediation. Therefore, biosurfactant-enhanced solubility of PAHs has potential applications in bioremediation.

  16. Effects of rhamnolipid biosurfactants on removal of phenanthrene from soil

    NARCIS (Netherlands)

    Noordman, Wouter H.; Ji, Wei; Brusseau, Mark L.; Janssen, Dick B.

    1998-01-01

    Solubilizing agents may enhance remediation of-soils contaminated with hydrophobic organic contaminants by diminishing sorption of the contaminants or increasing desorption rates. The effectiveness of rhamnolipid biosurfactants to enhance the removal of sorbed contaminants from soil was determined

  17. Bioconversion of agro-industrial by-products in rhamnolipids toward applications in enhanced oil recovery and bioremediation.

    Science.gov (United States)

    Gudiña, Eduardo J; Rodrigues, Ana I; Alves, Eliana; Domingues, M Rosário; Teixeira, José A; Rodrigues, Lígia R

    2015-02-01

    In this work, biosurfactant production by a Pseudomonas aeruginosa strain was optimized using low-cost substrates. The highest biosurfactant production (3.2 g/l) was obtained using a culture medium containing corn steep liquor (10% (v/v)) and molasses (10% (w/v)). The biosurfactant reduced the surface tension of water up to 30 mN/m, and exhibited a high emulsifying activity (E24=60%), with a critical micelle concentration as low as 50 mg/l. The biosurfactant produced in this alternative medium was characterized as a mixture of eight different rhamnolipid congeners, being the most abundant the mono-rhamnolipid Rha-C10-C10. However, using LB medium, nine different rhamnolipid congeners were identified, being the most abundant the di-rhamnolipid Rha-Rha-C10-C10. The rhamnolipid mixture produced in the alternative medium exhibited a better performance in removing oil from contaminated sand when compared with two chemical surfactants, suggesting its potential use as an alternative to traditional chemical surfactants in enhanced oil recovery or bioremediation. Copyright © 2014 Elsevier Ltd. All rights reserved.

  18. Optimizing Greenhouse Rice Production: Summary of Recommendations

    OpenAIRE

    Eddy, Robert; Acosta, Kevin; Liu, Yisi; Russell, Michael

    2016-01-01

    This publication provides a single-page chart summarizing our protocols for growing Rice (japonica). Split into three production goals, recommendations are given for photoperiod, temperature, lighting, container, root medium, planting density, irrigation, fertilization, algae control and fungus gnat control. This version updates our fertilization frequency, pot size, root medium and algae control recommendations. This document summarizes a series of questions and answers originally posted ...

  19. Production and characterization of rhamnolipids produced by Serratia rubidaea SNAU02 under solid-state fermentation and its application as biocontrol agent.

    Science.gov (United States)

    Nalini, S; Parthasarathi, R

    2014-12-01

    The present study aimed at exploring mahua (Madhuca indica) oil cake as a novel substrate for the production of biosurfactant by Serratia rubidaea SNAU02 under solid-state fermentation (SSF). Response surface methodology showed followings as the optimal conditions for the production of biosurfactant: mahua oil cake 7.48 g, 2.5 ml inoculum size (1×10(8) cells/ml), and pH 7.22 and 31 °C temperature. The characterization of the biosurfactant by TLC, FT-IR and GC-MS revealed the presence of rhamnolipid. The presence of rhamnosyl transferase gene responsible for biosynthesis of rhamnolipid was identified. The strain SNAU02 exhibited antifungal activity and demonstrated no toxicity against the seeds of Brassica oleracea and Artemia salina employed as a bio-indicator. The present findings indicated the potential of mahua oil cake as suitable substrate for the production of rhamnolipids in SSF by S. rubidaea SNAU02 and application potential of the biosurfactant produced as biocontrol agent against plant pathogens. Copyright © 2014 Elsevier Ltd. All rights reserved.

  20. Optimization of levan production by Zymomonas mobilis

    Directory of Open Access Journals (Sweden)

    V. K Ananthalakshmy

    1999-01-01

    Full Text Available Effect of different fermentation conditions on levan production by Zymomonas mobilis B-4286 was studied. Levan production increased from 5.7-g/l to 12.6-g/l with an increase in initial sucrose concentration (50-150 g/l. Above 15% (20 and 25% sucrose concentration, there was no increase in the biomass. The sucrose hydrolysis and levan production occurred even in the absence of significant growth of cells. Maximum amount of levan was produced (14.5 g/l at pH 5 and 15 g /l at 25(0C temperature. At temperature between 35(0C and 40(0C, levan production was not detected. Presence of glucose in the medium considerably reduced levan production (2.8 g/l than fructose 6.7g/l.O efeito de diferentes condições de fermentação na produção de levan por Zymomonas mobilis B-4286 foi estudado. A produção de Levan aumentou de 5.7-g/l a 12.6-g/l com o aumento da concentração inicial de sacarose (50-150 g/l. Acima de 15%, 20 e 25% a concentração de sacarose, não propiciou nenhum acréscimo na formação de biomassa. A hidrólise da sacarose e produção de Levan ocorreram de forma normal na ausência de um crescimento celular significativo. A concentração máxima de levan produzida foi (14.5 g/l em pH 5, 15 g /l a 25(0 C. Na temperatura entre 35(0C e 40(0 C, não ocorreu a produção de levan. A presença de glicose no meio de cultivo reduziu consideravelmente a produção média de levan (2.8 g/l bem como a de frutose (6.7g/l..

  1. OPTIMIZING PRODUCTIVE LAND WAQF TOWARDS FARMERS PROSPERITY

    Directory of Open Access Journals (Sweden)

    Ajeng Wahyu Puspitasari

    2017-05-01

    Full Text Available Data of The Ministry of Religion of Indonesia show that the total number of land waqf is 4,359 billion M2 in Indonesia (data of March, 2016. Based on this data, land waqf has a huge potential economics to be improved in Indonesia as community economic development. Land waqf, generally, is used to built a mosque, orphanage, and other public facilities. On the other side, the development of agriculture getting decrease because being converted into residence, especially in Indonesia as the agraris country. The use of land waqf is not maximum yet, therefore we have a big chance to cultivate the land waqf by using an Islamic concept of agriculture as one of the solution.             Realizing the importance of land waqf management, this study aims ensuring that land waqf can be managed by the local government and to be used as farmland by involving farmers as workers investigating  by using a literature review. The concept of land waqf is muzara'ah, there is an agreement between the local governance (as the manager of land waqf and farmers (as the workers to cultivate the land, then at the end of this agreement, the total yield will taken by the local government in order to fulfill the needs of the farmers. Optimizing the potential of land waqf in Indonesia, in order to reach the maximum benefit of waqf that called as falah.

  2. Utilization of Crude Glycerol as a Substrate for the Production of Rhamnolipid by Pseudomonas aeruginosa.

    Science.gov (United States)

    Eraqi, Walaa A; Yassin, Aymen S; Ali, Amal E; Amin, Magdy A

    2016-01-01

    Biosurfactants are produced by bacteria or yeast utilizing different substrates as sugars, glycerol, or oils. They have important applications in the detergent, oil, and pharmaceutical industries. Glycerol is the product of biodiesel industry and the existing glycerol market cannot accommodate the excess amounts generated; consequently, new markets for refined glycerol need to be developed. The aim of present work is to optimize the production of microbial rhamnolipid using waste glycerol. We have developed a process for the production of rhamnolipid biosurfactants using glycerol as the sole carbon source by a local Pseudomonas aeruginosa isolate that was obtained from an extensive screening program. A factorial design was applied with the goal of optimizing the rhamnolipid production. The highest production yield was obtained after 2 days when cells were grown in minimal salt media at pH 6, containing 1% (v/v) glycerol and 2% (w/v) sodium nitrate as nitrogen source, at 37°C and at 180 rpm, and reached 2.164 g/L after 54 hours (0.04 g/L h). Analysis of the produced rhamnolipids by TLC, HPLC, and FTIR confirmed the nature of the biosurfactant as monorhamnolipid. Glycerol can serve as a source for the production of rhamnolipid from microbial isolates providing a cheap and reliable substrate.

  3. Bioremediation of Pyrene-Contaminated Soils Using Biosurfactant

    Directory of Open Access Journals (Sweden)

    Jorfi

    2014-10-01

    Full Text Available Background Polycyclic aromatic hydrocarbons (PAHs are persistence organic chemicals with proved carcinogenic and mutagenic hazards. These compounds are usually adsorbed in soils in vicinity of oil and gas industries. Bioremediation of PAHs contaminated soils is difficult due to hydrophobic nature of PAHs. Objectives The main purpose of the current study was to determine the pyrene removal efficiency in synthetically contaminated soil, using biosurfactant. Materials and Methods Four pure bacterial strains capable of pyrene degradation were isolated from contaminated soils via enrichment techniques. The soil samples were spiked with an initial pyrene concentration of 500 mg/kg and subjected to bioremediation using a mixed culture comprised of previously isolated strains, in addition to application of biosurfactant during 63 days. Results The pyrene removal efficiency in samples containing biosurfactant, without biosurfactant and controls, were 86.4%, 59.8% and 14%, respectively, after 63 days. The difference of pyrene removal efficiency between the biosurfactant-containing samples and the ones without it was significant (P < 0.05. Conclusions Application of rhamnolipid biosurfactant produced by Pseudomonas aeruginosa significantly improved pyrene removal in contaminated soils.

  4. Optimization of alcohol production from Jerusalem artichokes

    Energy Technology Data Exchange (ETDEWEB)

    Guiraud, J.P.; Caillaud, J.M.; Galzy, P.

    1982-01-01

    Fermentation of Jerusalem artichoke extracts by yeasts with inulinase activity is possible, without prior hydrolysis or sterilization, if carried out at pH 3.5. For semi-continuous production, a small amount of the yeast harvested at the end of the previous fermentation can be used as the subsequent inoculant. Up to 75 hl of alcohol per ha can be obtained by this process under favorable energetic conditions. A partial inhibition of the fermentation was detected in extracts obtained from tubers harvested too early; this inhibition seems unrelated to the extent of polymerization of sugars. (Refs. 9).

  5. Optimization of alcohol production from Jerusalem artichokes

    Energy Technology Data Exchange (ETDEWEB)

    Guiraud, J.P.; Caillaud, J.M.; Galzy, P.

    1982-01-01

    Fermentation of Jerusalem artichoke extracts by yeasts with inulinase activity is possible, without prior hydrolysis or sterilization, if carried out at pH 3.5. For semi-continuous production, a small amount of the yeast harvested at the end of the previous fermentation can be used as the subsequent inoculant. Up to 75 hl of alcohol per ha can be obtained by this process under favorable energetic conditions. A partial inhibition of the fermentation was detected in extracts obtained from tubers harvested too early; this inhibition seems unrelated to the extent of polymerization of sugars.

  6. Jatropha Oil Derived Sophorolipids: Production and Characterization as Laundry Detergent Additive

    OpenAIRE

    Joshi-Navare, Kasturi; Khanvilkar, Poonam; Prabhune, Asmita

    2013-01-01

    Sophorolipids (SLs) are glycolipidic biosurfactants suitable for various biological and physicochemical applications. The nonedible Jatropha oil has been checked as the alternative raw material for SL synthesis using C. bombicola (ATCC22214). This is useful towards lowering the SL production cost. Through optimization of fermentation parameters and use of resting cell method, the yield 15.25 g/L could be achieved for Jatropha oil derived SL (SLJO) with 1% v/v oil feeding. The synthesized SL d...

  7. Simulation Based Optimization for World Line Card Production System

    Directory of Open Access Journals (Sweden)

    Sinan APAK

    2012-07-01

    Full Text Available Simulation based decision support system is one of the commonly used tool to examine complex production systems. The simulation approach provides process modules which can be adjusted with certain parameters by using data relatively easily obtainable in production process. World Line Card production system simulation is developed to evaluate the optimality of existing production line via using discrete event simulation model with variaty of alternative proposals. The current production system is analysed by a simulation model emphasizing the bottlenecks and the poorly utilized production line. Our analysis identified some improvements and efficient solutions for the existing system.

  8. Optimization of Fermentation Conditions for the Production of Bacteriocin Fermentate

    Science.gov (United States)

    2015-03-30

    FERMENTATION CONDITIONS FOR THE PRODUCTION OF BACTERIOCIN “ FERMENTATE ” by Anthony Sikes Wayne Muller and Claire Lee March 2015...From - To) October 2010 – November 2013 4. TITLE AND SUBTITLE OPTIMIZATION OF FERMENTATION CONDITIONS FOR THE PRODUCTION OF BACTERIOCIN “ FERMENTATE ...nisin and pediocin. Whey + yeast extract was the best performing whey fermentation media. The nisin producer strain Lactococcus. lactis ssp. lactis was

  9. Simulation-based optimization for product and process design

    NARCIS (Netherlands)

    Driessen, L.

    2006-01-01

    The design of products and processes has gradually shifted from a purely physical process towards a process that heavily relies on computer simulations (virtual prototyping). To optimize this virtual design process in terms of speed and final product quality, statistical methods and mathematical

  10. Optimization of cholesterol oxidase production by Brevibacterium sp ...

    African Journals Online (AJOL)

    An ultrasound-assisted emulsification as a pretreatment for cholesterol oxidase production by submerge fermentation using Brevibacterium sp. in a batch system was studied. Medium improvement for the production employing response surface methodology (RSM) was optimized in this paper. The concentration of ...

  11. Marketing research: Pathway to optimal market oriented products ...

    African Journals Online (AJOL)

    This paper examines the role of marketing research as pathway to developing optimal market oriented products. The objectives of this paper among others are to (i) Establish whether the use of marketing research has effect on new product adoption process by consumers (ii) Find out if there is any relationship between the ...

  12. Isolation and partial characterization of a biosurfactant produced by Streptococcus thermophilus A

    NARCIS (Netherlands)

    Rodrigues, Ligia R.; Teixeira, Jose A.; van der Mei, Henny C.; Oliveira, Rosario

    2006-01-01

    Isolation and characterization of the surface active components from the crude biosurfactant produced by Streptococcus thermophilus A was studied. A fraction rich in glycolipids was obtained by the fractionation of crude biosurfactant using hydrophobic interaction chromatography. Molecular (by

  13. Functional, genetic and chemical characterization of biosurfactants produced by plant growth-promoting Pseudomonas putida 267

    NARCIS (Netherlands)

    Kruijt, M.; Tran, H.; Raaijmakers, J.M.

    2009-01-01

    Aims: Plant growth-promoting Pseudomonas putida strain 267, originally isolated from the rhizosphere of black pepper, produces biosurfactants that cause lysis of zoospores of the oomycete pathogen Phytophthora capsici. The biosurfactants were characterized, the biosynthesis gene(s) partially

  14. Superstructure optimization of biodiesel production from microalgal biomass

    DEFF Research Database (Denmark)

    Rizwan, Muhammad; Lee, Jay H.; Gani, Rafiqul

    2013-01-01

    In this study, we propose a mixed integer nonlinear programming (MINLP) model for superstructure based optimization of biodiesel production from microalgal biomass. The proposed superstructure includes a number of major processing steps for the production of biodiesel from microalgal biomass...... for the production of biodiesel from microalgae. The proposed methodology is tested by implementing on a specific case study. The MINLP model is implemented and solved in GAMS using a database built in Excel. The results from the optimization are analyzed and their significances are discussed....

  15. Optimization of biodiesel production from castor oil.

    Science.gov (United States)

    da Silva, Nivea de Lima; Maciel, Maria Regina Wolf; Batistella, César Benedito; Maciel Filho, Rubens

    2006-01-01

    The transesterification of castor oil with ethanol in the presence of sodium ethoxide as catalyst is an exceptional option for the Brazilian biodiesel production, because the castor nut is quite available in the country. Chemically, its oil contains about 90% of ricinoleic acid that gives to the oil some beneficial characteristics such as its alcohol solubility at 30 degrees C. The transesterification variables studied in this work were reaction temperature, catalyst concentration and alcohol oil molar ratio. Through a star configuration experimental design with central points, this study shows that it is possible to achieve the same conversion of esters carrying out the transesterification reaction with a smaller alcohol quantity, and a new methodology was developed to obtain high purity biodiesel.

  16. The Process of Optimizing Mechanical Sound Quality in Product Design

    DEFF Research Database (Denmark)

    Eriksen, Kaare; Holst, Thomas

    2011-01-01

    The research field concerning optimizing product sound quality is a relatively unexplored area, and may become difficult for designers to operate in. To some degree, sound is a highly subjective parameter, which is normally targeted sound specialists. This paper describes the theoretical...... and practical background for managing a process of optimizing the mechanical sound quality in a product design by using simple tools and workshops systematically. The procedure is illustrated by a case study of a computer navigation tool (computer mouse or mouse). The process is divided into 4 phases, which...... clarify the importance of product sound, defining perceptive demands identified by users, and, finally, how to suggest mechanical principles for modification of an existing sound design. The optimized mechanical sound design is followed by tests on users of the product in its use context. The result...

  17. Jointly Production and Correlated Maintenance Optimization for Parallel Leased Machines

    Directory of Open Access Journals (Sweden)

    Tarek ASKRI

    2017-04-01

    Full Text Available This paper deals with a preventive maintenance strategy optimization correlated to production for a manufacturing system made by several parallel machines under lease contract. In order to minimize the total cost of production and maintenance by reducing the production system interruptions due to maintenance activities, a correlated group preventive maintenance policy is developed using the gravity center approach (GCA. The aim of this study is to determine an economical production plan and an optimal group preventive maintenance interval Tn at which all machines are maintained simultaneously. An analytical correlation between failure rate of machines and production level is considered and the impact of the preventive maintenance policy on the production plan is studied. Finally, the proposed maintenance policy GPM is compared with an individual simple strategy approach IPM in order to illustrate its efficiency.

  18. [Optimization of the pertussis vaccine production process].

    Science.gov (United States)

    Germán Santiago, J; Zamora, N; de la Rosa, E; Alba Carrión, C; Padrón, P; Hernández, M; Betancourt, M; Moretti, N

    1995-01-01

    The production of Pertussis Vaccine was reevaluated at the Instituto Nacional de Higiene "Rafael Rangel" in order to optimise it in terms of vaccine yield, potency, specific toxicity and efficiency (cost per doses). Four different processes, using two culture media (Cohen-Wheeler and Fermentación Glutamato Prolina-1) and two types of bioreactors (25 L Fermentador Caracas and a 450 L industrial fermentor) were compared. Runs were started from freeze-dried strains (134 or 509) and continued until the obtention of the maximal yield. It was found that the combination Fermentación Glutamato Prolina-1/industrial fermentor, shortened the process to 40 hours while consistently yielding a vaccine of higher potency (7.91 +/- 2.56 IU/human dose) and lower specific toxicity in a mice bioassay. In addition, the physical aspect of the preparation was rather homogeneous and free of dark aggregates. Most importantly, the biomass yield more than doubled those of the Fermentador Caracas using the two different media and that in the industrial fermentor with the Cohen-Wheeler medium. Therefore, the cost per doses was substantially decreased.

  19. Produção de biotensoativos a partir de resíduos de óleos e gorduras Fats and oils wastes as substrates for biosurfactant production

    Directory of Open Access Journals (Sweden)

    Siddhartha Georges Valadares Almeida de Oliveira Costa

    2008-03-01

    Full Text Available O presente trabalho visou a seleção de microrganismos com capacidade de produzir biotensoativos a partir de resíduos de óleos e gorduras gerados em restaurantes e indústrias alimentícias. Borra de soja, gordura de frango, gordura vegetal hidrogenada e óleo de soja usado em frituras foram estudados como fonte de carbono. Os isolados LMI 6c e LMI 7a, ambos pertencentes ao gênero Pseudomonas, foram selecionados como potenciais produtores de biotensoativos. Dentre os resíduos propostos, a borra de soja foi considerada o melhor substrato, gerando 9,69 g.L-1 de ramnolipídios e uma tensão superficial de 31 mN/m.The purpose of this study was to select microorganisms that were able to produce biosurfactants from fats and oils wastes generated by the food industry and restaurants. Soybean soapstock, chicken fat, hydrogenated vegetable fat and soybean frying oil were evaluated as alternative substrates. Pseudomonas sp. isolates LMI 6c and LMI 7a showed a capacity to utilize the substrates and to produce rhamnolipid surfactants. Soybean soapstock was considered the best substrate, generating 9.69 g.L-1 of rhamnolipids and a surface tension of 31 mN/m.

  20. Economic optimization of waste treatment and energy production in Denmark

    DEFF Research Database (Denmark)

    Münster, Marie; Ravn, Hans; Hedegaard, Karsten

    2013-01-01

    This article presents an optimization model that incorporates LCA methodology and captures important characteristics of waste management systems. The most attractive waste management options are in the model identified as part the optimization. The model renders it possible to apply different...... optimization objectives such as minimizing costs or greenhouse gas emissions or to prioritise several objectives given different weights. An illustrative case is analyzed, covering alternative treatments of 1 tonne residual household waste: incineration of the full amount or sorting out organic waste...... for biogas production for either CHP generation or as fuel in vehicles. The case study illustrates, that what is the optimal solution depends on the objective and assumptions regarding the background system – here illustrated with different assumptions regarding displaced electricity production. The article...

  1. Biosurfactant gene clusters in eukaryotes: regulation and biotechnological potential.

    Science.gov (United States)

    Roelants, Sophie L K W; De Maeseneire, Sofie L; Ciesielska, Katarzyna; Van Bogaert, Inge N A; Soetaert, Wim

    2014-04-01

    Biosurfactants (BSs) are a class of secondary metabolites representing a wide variety of structures that can be produced from renewable feedstock by a wide variety of micro-organisms. They have (potential) applications in the medical world, personal care sector, mining processes, food industry, cosmetics, crop protection, pharmaceuticals, bio-remediation, household detergents, paper and pulp industry, textiles, paint industries, etc. Especially glycolipid BSs like sophorolipids (SLs), rhamnolipids (RLs), mannosylerythritol lipids (MELs) and cellobioselipids (CBLs) have been described to provide significant opportunities to (partially) replace chemical surfactants. The major two factors currently limiting the penetration of BSs into the market are firstly the limited structural variety and secondly the rather high production price linked with the productivity. One of the keys to resolve the above mentioned bottlenecks can be found in the genetic engineering of natural producers. This could not only result in more efficient (economical) recombinant producers, but also in a diversification of the spectrum of available BSs as such resolving both limiting factors at once. Unraveling the genetics behind the biosynthesis of these interesting biological compounds is indispensable for the tinkering, fine tuning and rearrangement of these biological pathways with the aim of obtaining higher yields and a more extensive structural variety. Therefore, this review focuses on recent developments in the investigation of the biosynthesis, genetics and regulation of some important members of the family of the eukaryotic glycolipid BSs (MELs, CBLs and SLs). Moreover, recent biotechnological achievements and the industrial potential of engineered strains are discussed.

  2. Numerical aspects of optimal control of penicillin production

    Czech Academy of Sciences Publication Activity Database

    Pčolka, M.; Čelikovský, Sergej

    2014-01-01

    Roč. 37, č. 1 (2014), s. 71-81 ISSN 1615-7591 R&D Projects: GA ČR(CZ) GA13-20433S Institutional support: RVO:67985556 Keywords : Optimal control * Nonlinear systems * Fermentation process * Gradient method optimization * Antibiotics production Subject RIV: BC - Control Systems Theory Impact factor: 1.997, year: 2014 http://library.utia.cas.cz/separaty/2014/TR/celikovsky-0424718.pdf

  3. Regularity of optimal transport maps on multiple products of spheres

    OpenAIRE

    Figalli, Alessio; Kim, Young-Heon; McCann, Robert J.

    2010-01-01

    This article addresses regularity of optimal transport maps for cost="squared distance" on Riemannian manifolds that are products of arbitrarily many round spheres with arbitrary sizes and dimensions. Such manifolds are known to be non-negatively cross-curved [KM2]. Under boundedness and non-vanishing assumptions on the transfered source and target densities we show that optimal maps stay away from the cut-locus (where the cost exhibits singularity), and obtain injectivity and continuity of o...

  4. Portfolio optimization with structured products under return constraint

    Directory of Open Access Journals (Sweden)

    Baweja Meena

    2015-01-01

    Full Text Available A new approach for optimizing risk in a portfolio of financial instruments involving structured products is presented. This paper deals with a portfolio selection model which uses optimization methodology to minimize conditional Value-at-Risk (CVaR under return constraint. It focuses on minimizing CVaR rather than on minimizing value-at-Risk VaR, as portfolios with low CVaR necessarily have low VaR as well. We consider a simple investment problem where besides stocks and bonds, the investor can also include structured products into the investment portfolio. Due to possible intermediate payments from structured product, we have to deal with a re-investment problem modeled as a linear optimization problem.

  5. Modular production line optimization: The exPLORE architecture

    Directory of Open Access Journals (Sweden)

    Spinellis Diomidis D.

    2000-01-01

    Full Text Available The general design problem in serial production lines concerns the allocation of resources such as the number of servers, their service rates, and buffers given production-specific constraints, associated costs, and revenue projections. We describe the design of exPLOre: a modular, object-oriented, production line optimization software architecture. An abstract optimization module can be instantiated using a variety of stochastic optimization methods such as simulated annealing and genetic algorithms. Its search space is constrained by a constraint checker while its search direction is guided by a cost analyser which combines the output of a throughput evaluator with the business model. The throughput evaluator can be instantiated using Markovian, generalised queueing network methods, a decomposition, or an expansion method algorithm.

  6. Optimization and Improvement of Test Processes on a Production Line

    Science.gov (United States)

    Sujová, Erika; Čierna, Helena

    2018-06-01

    The paper deals with increasing processes efficiency at a production line of cylinder heads of engines in a production company operating in the automotive industry. The goal is to achieve improvement and optimization of test processes on a production line. It analyzes options for improving capacity, availability and productivity of processes of an output test by using modern technology available on the market. We have focused on analysis of operation times before and after optimization of test processes at specific production sections. By analyzing measured results we have determined differences in time before and after improvement of the process. We have determined a coefficient of efficiency OEE and by comparing outputs we have confirmed real improvement of the process of the output test of cylinder heads.

  7. Optimal Maintenance of a Production System with Intermediate Buffers

    Directory of Open Access Journals (Sweden)

    Constantinos C. Karamatsoukis

    2012-01-01

    Full Text Available We consider a production-inventory system that consists of an input-generating installation, a production unit and L intermediate buffers. It is assumed that the installation transfers the raw material to buffer and the production unit pulls the raw material from buffer We consider the problem of the optimal preventive maintenance of the installation if the installation deteriorates stochastically with usage and the production unit is always in operative condition. We also consider the problem of the optimal preventive maintenance of the production unit if the production unit deteriorates stochastically with usage and the installation is always in operative condition. Under a suitable cost structure and for given contents of the buffers, it is proved that the average-cost optimal policy for the first (second problem initiates a preventive maintenance of the installation (production unit if and only if the degree of deterioration of the installation (production unit exceeds some critical level. Numerical results are presented for both problems.

  8. Robust Optimization Model for Production Planning Problem under Uncertainty

    Directory of Open Access Journals (Sweden)

    Pembe GÜÇLÜ

    2017-01-01

    Full Text Available Conditions of businesses change very quickly. To take into account the uncertainty engendered by changes has become almost a rule while planning. Robust optimization techniques that are methods of handling uncertainty ensure to produce less sensitive results to changing conditions. Production planning, is to decide from which product, when and how much will be produced, with a most basic definition. Modeling and solution of the Production planning problems changes depending on structure of the production processes, parameters and variables. In this paper, it is aimed to generate and apply scenario based robust optimization model for capacitated two-stage multi-product production planning problem under parameter and demand uncertainty. With this purpose, production planning problem of a textile company that operate in İzmir has been modeled and solved, then deterministic scenarios’ and robust method’s results have been compared. Robust method has provided a production plan that has higher cost but, will result close to feasible and optimal for most of the different scenarios in the future.

  9. Resource-based optimization of electric power production (in Iran)

    International Nuclear Information System (INIS)

    Sadeghzadeh, Mohammad

    1999-01-01

    This paper is about electric power production optimization and chiefly discusses on the types of resources available in Iran. The modeling has been based on the marginal cost of different energy resources and types of technologies used. the computed costs are the basic standards for optimization of the production system of energy. the costs associated with environmental pollution and also pollution control are considered. the present paper also studied gas fossil fuel, hydro, nuclear, renewable and co-generation of heat and power. The results are discussed and reported at the last of the paper

  10. Antibiotic and biosurfactant properties of cyclic lipopeptides produced by fluorescent Pseudomonas spp. from the sugar beet rhizosphere

    DEFF Research Database (Denmark)

    Nielsen, T.H.; Sørensen, D.; Tobiasen, C.

    2002-01-01

    Cyclic lipopeptides (CLPs) with antibiotic and biosurfactant properties are produced by a number of soil bacteria, including fluorescent Pseudomonas spp. To provide new and efficient strains for the biological control of root-pathogenic fungi in agricultural crops, we isolated approximately 600...... fluorescent Pseudomonas spp. from two different agricultural soils by using three different growth media. CLP production was observed in a large proportion of the strains (approximately 60%) inhabiting the sandy soil, compared to a low proportion (approximately 6%) in the loamy soil. Chemical structure...... in the peptide moiety. Production of specific CLPs could be affiliated with Pseudomonas fluorescens strain groups belonging to biotype I, V, or VI. In vitro analysis using both purified CLPs and whole-cell P. fluorescens preparations demonstrated that all CLPs exhibited strong biosurfactant properties...

  11. Removal of mercury by foam fractionation using surfactin, a biosurfactant.

    Science.gov (United States)

    Chen, Hau-Ren; Chen, Chien-Cheng; Reddy, A Satyanarayana; Chen, Chien-Yen; Li, Wun Rong; Tseng, Min-Jen; Liu, Hung-Tsan; Pan, Wei; Maity, Jyoti Prakash; Atla, Shashi B

    2011-01-01

    The separation of mercury ions from artificially contaminated water by the foam fractionation process using a biosurfactant (surfactin) and chemical surfactants (SDS and Tween-80) was investigated in this study. Parameters such as surfactant and mercury concentration, pH, foam volume, and digestion time were varied and their effects on the efficiency of mercury removal were investigated. The recovery efficiency of mercury ions was highly sensitive to the concentration of the surfactant. The highest mercury ion recovery by surfactin was obtained using a surfactin concentration of 10 × CMC, while recovery using SDS required 10 × CMC. However, the enrichment of mercury ions in the foam was superior with surfactin, the mercury enrichment value corresponding to the highest metal recovery (10.4%) by surfactin being 1.53. Dilute solutions (2-mg L(-1) Hg(2+)) resulted in better separation (36.4%), while concentrated solutions (100 mg L(-1)) enabled only a 2.3% recovery using surfactin. An increase in the digestion time of the metal solution with surfactin yielded better separation as compared with a freshly-prepared solution, and an increase in the airflow rate increased bubble production, resulting in higher metal recovery but low enrichment. Basic solutions yielded higher mercury separation as compared with acidic solutions due to the precipitation of surfactin under acidic conditions.

  12. Removal of Mercury by Foam Fractionation Using Surfactin, a Biosurfactant

    Directory of Open Access Journals (Sweden)

    Shashi B. Atla

    2011-11-01

    Full Text Available The separation of mercury ions from artificially contaminated water by the foam fractionation process using a biosurfactant (surfactin and chemical surfactants (SDS and Tween-80 was investigated in this study. Parameters such as surfactant and mercury concentration, pH, foam volume, and digestion time were varied and their effects on the efficiency of mercury removal were investigated. The recovery efficiency of mercury ions was highly sensitive to the concentration of the surfactant. The highest mercury ion recovery by surfactin was obtained using a surfactin concentration of 10 × CMC, while recovery using SDS required < 10 × CMC and Tween-80 >10 × CMC. However, the enrichment of mercury ions in the foam was superior with surfactin, the mercury enrichment value corresponding to the highest metal recovery (10.4% by surfactin being 1.53. Dilute solutions (2-mg L−1 Hg2+ resulted in better separation (36.4%, while concentrated solutions (100 mg L−1 enabled only a 2.3% recovery using surfactin. An increase in the digestion time of the metal solution with surfactin yielded better separation as compared with a freshly-prepared solution, and an increase in the airflow rate increased bubble production, resulting in higher metal recovery but low enrichment. Basic solutions yielded higher mercury separation as compared with acidic solutions due to the precipitation of surfactin under acidic conditions.

  13. The anionic biosurfactant rhamnolipid does not denature industrial enzymes

    Directory of Open Access Journals (Sweden)

    Jens Kvist Madsen

    2015-04-01

    Full Text Available Biosurfactants (BS are surface-active molecules produced by microorganisms. Their combination of useful properties and sustainable production make them promising industrial alternatives to petrochemical and oleochemical surfactants. Here we compare the impact of the anionic BS rhamnolipid (RL and the conventional/synthetic anionic surfactant sodium dodecyl sulfate (SDS on the structure and stability of three different commercially used enzymes, namely the cellulase Carezyme® (CZ, the phospholipase Lecitase Ultra® (LT and the α-amylase Stainzyme® (SZ. Our data reveal a fundamental difference in their mode of interaction. SDS shows great diversity of interaction towards the different enzymes. It efficiently unfolds both LT and CZ, but LT is unfolded by SDS through formation of SDS clusters on the protein well below the cmc, while CZ is only unfolded by bulk micelles and on average binds significantly less SDS than LT. SDS binds with even lower stoichiometry to SZ and leads to an increase in thermal stability. In contrast, RL does not affect the tertiary or secondary structure of any enzyme at room temperature, has little impact on thermal stability and only binds detectably (but at low stoichiometries to SZ. Furthermore all enzymes maintain activity at both monomeric and micellar concentrations of RL. We conclude that RL, despite its anionic charge, is a surfactant that does not compromise the structural integrity of industrially relevant proteins. This makes RL a promising alternative to current synthetic anionic surfactants in a wide range of commercial applications.

  14. Optimal Pricing and Advertising Policies for New Product Oligopoly Models

    OpenAIRE

    Gerald L. Thompson; Jinn-Tsair Teng

    1984-01-01

    In this paper our previous work on monopoly and oligopoly new product models is extended by the addition of pricing as well as advertising control variables. These models contain Bass's demand growth model, and the Vidale-Wolfe and Ozga advertising models, as well as the production learning curve model and an exponential demand function. The problem of characterizing an optimal pricing and advertising policy over time is an important question in the field of marketing as well as in the areas ...

  15. Innovations for production optimization in the petroleum industry: conference reports

    International Nuclear Information System (INIS)

    Anon.

    1997-01-01

    This Insight conference was held to review the methods by which the petroleum industry could optimize production. Presentations from nine guest speakers were included. The issues addressed included the use of computer software for integrated data systems such as SCADA and GIS, the use of remote sensing and real-time systems to monitor well production and reserves capability more effectively, and innovations to minimize finding and development costs and their effect on financial markets. figs

  16. Optimization for routing vehicles of seafood product transportation

    Science.gov (United States)

    Soenandi, I. A.; Juan, Y.; Budi, M.

    2017-12-01

    Recently, increasing usage of marine products is creating new challenges for businesses of marine products in terms of transportation that used to carry the marine products like seafood to the main warehouse. This can be a problem if the carrier fleet is limited, and there are time constraints in terms of the freshness of the marine product. There are many ways to solve this problem, including the optimization of routing vehicles. In this study, this strategy is to implement in the marine product business in Indonesia with such an expected arrangement of the company to optimize routing problem in transportation with time and capacity windows. Until now, the company has not used the scientific method to manage the routing of their vehicle from warehouse to the location of marine products source. This study will solve a stochastic Vehicle Routing Problems (VRP) with time and capacity windows by using the comparison of six methods and looking the best results for the optimization, in this situation the company could choose the best method, in accordance with the existing condition. In this research, we compared the optimization with another method such as branch and bound, dynamic programming and Ant Colony Optimization (ACO). Finally, we get the best result after running ACO algorithm with existing travel time data. With ACO algorithm was able to reduce vehicle travel time by 3189.65 minutes, which is about 23% less than existing and based on consideration of the constraints of time within 2 days (including rest time for the driver) using 28 tons capacity of truck and the companies need two units of vehicles for transportation.

  17. Joint optimization of production scheduling and machine group preventive maintenance

    International Nuclear Information System (INIS)

    Xiao, Lei; Song, Sanling; Chen, Xiaohui; Coit, David W.

    2016-01-01

    Joint optimization models were developed combining group preventive maintenance of a series system and production scheduling. In this paper, we propose a joint optimization model to minimize the total cost including production cost, preventive maintenance cost, minimal repair cost for unexpected failures and tardiness cost. The total cost depends on both the production process and the machine maintenance plan associated with reliability. For the problems addressed in this research, any machine unavailability leads to system downtime. Therefore, it is important to optimize the preventive maintenance of machines because their performance impacts the collective production processing associated with all machines. Too lengthy preventive maintenance intervals may be associated with low scheduled machine maintenance cost, but may incur expensive costs for unplanned failure due to low machine reliability. Alternatively, too frequent preventive maintenance activities may achieve the desired high reliability machines, but unacceptably high scheduled maintenance cost. Additionally, product scheduling plans affect tardiness and maintenance cost. Two results are obtained when solving the problem; the optimal group preventive maintenance interval for machines, and the assignment of each job, including the corresponding start time and completion time. To solve this non-deterministic polynomial-time problem, random keys genetic algorithms are used, and a numerical example is solved to illustrate the proposed model. - Highlights: • Group preventive maintenance (PM) planning and production scheduling are jointed. • Maintenance interval and assignment of jobs are decided by minimizing total cost. • Relationships among system age, PM, job processing time are quantified. • Random keys genetic algorithms (GA) are used to solve the NP-hard problem. • Random keys GA and Particle Swarm Optimization (PSO) are compared.

  18. Biosurfactants Produced by Marine Microorganisms with Therapeutic Applications.

    Science.gov (United States)

    Gudiña, Eduardo J; Teixeira, José A; Rodrigues, Lígia R

    2016-02-18

    Marine microorganisms possess unique metabolic and physiological features and are an important source of new biomolecules, such as biosurfactants. Some of these surface-active compounds synthesized by marine microorganisms exhibit antimicrobial, anti-adhesive and anti-biofilm activity against a broad spectrum of human pathogens (including multi-drug resistant pathogens), and could be used instead of existing drugs to treat infections caused by them. In other cases, these biosurfactants show anti-cancer activity, which could be envisaged as an alternative to conventional therapies. However, marine biosurfactants have not been widely explored, mainly due to the difficulties associated with the isolation and growth of their producing microorganisms. Culture-independent techniques (metagenomics) constitute a promising approach to study the genetic resources of otherwise inaccessible marine microorganisms without the requirement of culturing them, and can contribute to the discovery of novel biosurfactants with significant biological activities. This paper reviews the most relevant biosurfactants produced by marine microorganisms with potential therapeutic applications and discusses future perspectives and opportunities to discover novel molecules from marine environments.

  19. Biosurfactants Produced by Marine Microorganisms with Therapeutic Applications

    Directory of Open Access Journals (Sweden)

    Eduardo J. Gudiña

    2016-02-01

    Full Text Available Marine microorganisms possess unique metabolic and physiological features and are an important source of new biomolecules, such as biosurfactants. Some of these surface-active compounds synthesized by marine microorganisms exhibit antimicrobial, anti-adhesive and anti-biofilm activity against a broad spectrum of human pathogens (including multi-drug resistant pathogens, and could be used instead of existing drugs to treat infections caused by them. In other cases, these biosurfactants show anti-cancer activity, which could be envisaged as an alternative to conventional therapies. However, marine biosurfactants have not been widely explored, mainly due to the difficulties associated with the isolation and growth of their producing microorganisms. Culture-independent techniques (metagenomics constitute a promising approach to study the genetic resources of otherwise inaccessible marine microorganisms without the requirement of culturing them, and can contribute to the discovery of novel biosurfactants with significant biological activities. This paper reviews the most relevant biosurfactants produced by marine microorganisms with potential therapeutic applications and discusses future perspectives and opportunities to discover novel molecules from marine environments.

  20. Nonlinear adaptive optimization of biomass productivity in continuous bioreactors

    Energy Technology Data Exchange (ETDEWEB)

    Sauvaire, P; Mellichamp, D A; Agrawal, P [California Univ., Santa Barbara, CA (United States). Dept. of Chemical and Nuclear Engineering

    1991-11-01

    A novel on-line adaptive optimization algorithm is developed and applied to continuous biological reactors. The algorithm makes use of a simple nonlinear estimation model that relates either the cell-mass productivity or the cell-mass concentration to the dilution rate. On-line estimation is used to recursively identify the parameters in the nonlinear process model and to periodically calculate and steer the bioreactor to the dilution rate that yields optimum cell-mass productivity. Thus, the algorithm does not require an accurate process model, locates the optimum dilution rate online, and maintains the bioreactors at this optimum condition at all times. The features of the proposed new algorithm are compared with those of other adaptive optimization techniques presented in the literature. A detailed simulation study using three different microbial system models was conducted to illustrate the performance of the optimization algorithms. (orig.).

  1. Optimal number of tests to achieve and validate product reliability

    International Nuclear Information System (INIS)

    Ahmed, Hussam; Chateauneuf, Alaa

    2014-01-01

    The reliability validation of engineering products and systems is mandatory for choosing the best cost-effective design among a series of alternatives. Decisions at early design stages have a large effect on the overall life cycle performance and cost of products. In this paper, an optimization-based formulation is proposed by coupling the costs of product design and validation testing, in order to ensure the product reliability with the minimum number of tests. This formulation addresses the question about the number of tests to be specified through reliability demonstration necessary to validate the product under appropriate confidence level. The proposed formulation takes into account the product cost, the failure cost and the testing cost. The optimization problem can be considered as a decision making system according to the hierarchy of structural reliability measures. The numerical examples show the interest of coupling design and testing parameters. - Highlights: • Coupled formulation for design and testing costs, with lifetime degradation. • Cost-effective testing optimization to achieve reliability target. • Solution procedure for nested aleatoric and epistemic variable spaces

  2. Ensemble based multi-objective production optimization of smart wells

    NARCIS (Netherlands)

    Fonseca, R.M.; Leeuwenburgh, O.; Jansen, J.D.

    2012-01-01

    In a recent study two hierarchical multi-objective methods were suggested to include short-term targets in life-cycle production optimization. However this previous study has two limitations: 1) the adjoint formulation is used to obtain gradient information, requiring simulator source code access

  3. Statistical optimization of process parameters for the production of ...

    African Journals Online (AJOL)

    In this study, optimization of process parameters such as moisture content, incubation temperature and initial pH (fixed) for the improvement of citric acid production from oil palm empty fruit bunches through solid state bioconversion was carried out using traditional one-factor-at-a-time (OFAT) method and response surface ...

  4. Product quality driven design of bakery operations using dynamic optimization

    NARCIS (Netherlands)

    Hadiyanto, M.; Esveld, D.C.; Boom, R.M.; Straten, van G.; Boxtel, van A.J.B.

    2008-01-01

    Abstract Quality driven design uses specified product qualities as a starting point for process design. By backward reasoning the required process conditions and processing system were found. In this work dynamic optimization was used as a tool to generate processing solutions for baking processes

  5. Statistical optimization of xylanase production by Aspergillus niger ...

    African Journals Online (AJOL)

    SERVER

    2008-03-04

    Mar 4, 2008 ... 2Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, P.R. China. 3School of ... of the main factors determining the economic of a process. Reducing the costs of enzyme production by optimization ..... tation periods during the potential industry application by.

  6. Medium optimization for protopectinase production by batch culture ...

    African Journals Online (AJOL)

    ajl yemi

    2011-11-11

    Nov 11, 2011 ... Optimization of medium compositions for protopectinase production by ... food industry, pharmacy and cosmetic manufacture due to ... energy intensive and industrial wastes (Iglesias and ... group of enzymes, which produce the enzymatic ... development time and overall costs (Pan et al., 2008; Ren.

  7. Behaviour - The keystone in optimizing free-ranging ungulate production

    Science.gov (United States)

    Free-ranging animal behaviour is a keystone to optimizing free-ranging domestic animal production. This chapter focuses on several aspects that emanate from foraging including defining terms, concepts and the complexity that underlie managing animals and landscapes. Behaviour is investigated in li...

  8. Selection and optimization of extracellular lipase production using ...

    African Journals Online (AJOL)

    The aim of this study was to isolate and select lipase-producing microorganisms originated from different substrates, as well as to optimize the production of microbial lipase by submerged fermentation under different nutrient conditions. Of the 40 microorganisms isolated, 39 showed a halo around the colonies and 4 were ...

  9. Energy-Performance as a driver for optimal production planning

    International Nuclear Information System (INIS)

    Salahi, Niloofar; Jafari, Mohsen A.

    2016-01-01

    Highlights: • A 2-dimensional Energy-Performance measure is proposed for energy aware production. • This is a novel approach integrates energy efficiency with production requirements. • This approach simultaneously incorporates machine and process related specifications. • The problem is solved as stochastic MILP with constraints addressing risk averseness. • The optimization is illustrated for 2 cases of single and serial machining operation. • Impact of various electricity pricing schemes on proposed production plan is analyzed. - Abstract: In this paper, we present energy-aware production planning using a two-dimensional “Energy-Performance” measure. With this measure, the production plan explicitly takes into account machine-level requirements, process control strategies, product types and demand patterns. The “Energy-Performance” measure is developed based on an existing concept, namely, “Specific Energy” at machine level. It is further expanded to an “Energy-Performance” profile for a production line. A production planning problem is formulated as a stochastic MILP with risk-averse constraints to account for manufacturer’s risk averseness. The objective is to attain an optimal production plan that minimizes the total loss distribution subject to system throughput targets, probabilistic risk constraints and constraints imposed by the underlying “Energy-Performance” pattern. Electricity price and demand per unit time are assumed to be stochastic. Conditional Value at Risk (CVaR) of loss distributions is used as the manufacturer’s risk measure. Both single-machine and production lines are studied for different profiles and electricity pricing schemes. It is shown that the shape of “Energy-Performance” profile can change optimal plans.

  10. Evaluation of biological properties and fate in the environment of a new class of biosurfactants.

    Science.gov (United States)

    Liwarska-Bizukojc, Ewa; Olejnik, Dorota; Delbeke, Elisabeth I P; Van Geem, Kevin M; Stevens, Christian V

    2018-06-01

    Selected sophorolipid quaternary ammonium salts (SQAS), being a new class of modified biosurfactants, were studied in this work for the first time with regard to their biodegradability and fate in the environment. It was made to find whether environment-friendly bioproducts like biosurfactants are still safe to the environment after their chemical modification. The susceptibility of these SQAS for biodegradation was estimated together with the evaluation of their influence on activated sludge microorganisms. Additionally, the mechanisms of removal of the SQAS from wastewater and from the aquatic environment, were analysed. The evaluated SQAS were potentially biodegradable, although none of them could be classified as readily biodegradable. The biodegradation degrees after 28 days ranged from 4 to 42%, dependent on the SQAS tested, i.e. below the required OECD 301D Closed Bottle Test level of 60%. Simultaneously, the analysis of the mass spectra revealed the presence of the breakdown products of each SQAS studied. Biodegradation was preceded by sorption of the SQAS on sludge particles, which occurred to be a main mechanism of the removal of these newly synthesized biosurfactants from wastewater. The mean degree of sorption calculated on the basis of SQAS determination was from 75 to 96%, dependent on the studied SQAS. The presence of SQAS in wastewater did not deteriorate the operation of the activated sludge system, although the products of the SQAS biodegradation remained in the liquid phase and might contribute to the increase of COD of the effluent to be introduced to the environment. Copyright © 2018 Elsevier Ltd. All rights reserved.

  11. Natural emulsifiers - Biosurfactants, phospholipids, biopolymers, and colloidal particles: Molecular and physicochemical basis of functional performance.

    Science.gov (United States)

    McClements, David Julian; Gumus, Cansu Ekin

    2016-08-01

    There is increasing consumer pressure for commercial products that are more natural, sustainable, and environmentally friendly, including foods, cosmetics, detergents, and personal care products. Industry has responded by trying to identify natural alternatives to synthetic functional ingredients within these products. The focus of this review article is on the replacement of synthetic surfactants with natural emulsifiers, such as amphiphilic proteins, polysaccharides, biosurfactants, phospholipids, and bioparticles. In particular, the physicochemical basis of emulsion formation and stabilization by natural emulsifiers is discussed, and the benefits and limitations of different natural emulsifiers are compared. Surface-active polysaccharides typically have to be used at relatively high levels to produce small droplets, but the droplets formed are highly resistant to environmental changes. Conversely, surface-active proteins are typically utilized at low levels, but the droplets formed are highly sensitive to changes in pH, ionic strength, and temperature. Certain phospholipids are capable of producing small oil droplets during homogenization, but again the droplets formed are highly sensitive to changes in environmental conditions. Biosurfactants (saponins) can be utilized at low levels to form fine oil droplets that remain stable over a range of environmental conditions. Some nature-derived nanoparticles (e.g., cellulose, chitosan, and starch) are effective at stabilizing emulsions containing relatively large oil droplets. Future research is encouraged to identify, isolate, purify, and characterize new types of natural emulsifier, and to test their efficacy in food, cosmetic, detergent, personal care, and other products. Copyright © 2016 Elsevier B.V. All rights reserved.

  12. Diversity of biosurfactant producing microorganisms isolated from soils contaminated with diesel oil.

    Science.gov (United States)

    Menezes Bento, Fátima; de Oliveira Camargo, Flavio A; Okeke, Benedict C; Frankenberger, William T

    2005-01-01

    Biosurfactant production is a desirable property of hydrocarbon-degrading microorganisms (HDM). We characterized biosurfactant producing microbial populations from a Long Beach soil, California (USA) and a Hong Kong soil (China), contaminated with diesel oil. A total of 33 hydrocarbon-utilizing microorganisms were isolated from the soils. Twelve isolates and three defined consortia were tested for biosurfactant production and emulsification activity. The highest reduction of surface tension was achieved with a consortium of L1, L2 and L3 isolates from a Long Beach soil (41.4mN m(-1)). Isolate L1 (Acinetobacter junii) displayed the highest reduction of surface tension (46.5 mN m(-1)). The emulsifying capacity evaluated by the E24 emulsification index was highest in the culture of isolate L5 (74%). No substantial emulsification was achieved with the cell-free extracts, indicating that the emulsifying activity was not extracellular. Based on surface tension and the E24 index results, isolates F1, F2, F3, F4, L1, L2, L3 and L4 were identified by 16S rRNA gene sequencing as Bacillus cereus, Bacillus sphaericus, B. fusiformis, Acinetobacter junii, a non-cultured bacterium, Pseudomonas sp. and B. pumilus, respectively. Cluster analyses of 16S rRNA gene sequences of the bacterial isolates revealed 70% similarity amongst hydrocarbon-degrading bacterial community present in both soils. Five isolates (isolates F1, F2, F3, F4 and L4) belong to the Firmicutes order, two isolates (L1 and L3) belong to the Proteobacteria order and one isolate (L2) is an Actinomyces sp. Simpson's index (1 - D) and the Shannon-Weaver index (H) revealed more diversity of HDM in the Hong Kong soil, while evenness (E) and the equitability (J) data indicated that there was not a dominant population. Bacterial isolates displaying substantial potential for production of biosurfactants can be applied in the bioremediation of soils contaminated with petroleum hydrocarbons.

  13. Optimization of SAGD wellbore completions : short production tubing string sensitivities

    Energy Technology Data Exchange (ETDEWEB)

    Cokar, M.; Graham, J. [Society of Petroleum Engineers, Canadian Section, Calgary, AB (Canada)]|[Petro-Canada, Calgary, AB (Canada)

    2008-10-15

    This study investigated the effects of changing the landing position of short production tubing strings near the heel of steam assisted gravity drainage (SAGD) production wells. A homogenous discretized wellbore model was used to model the reservoir and wellbore simultaneously in order to study wellbore and reservoir interactions. The aim of the study was to develop a method of optimizing bitumen production and determining the most economical position for wellbore strings. Simulations were conducted to examine the effect of shortening the production tubing string and examine the impact of extending the tubing string beyond the heel of the well on bitumen bitumen production rates and the steam oil ratio (SOR). Results of the study showed that a shortened string decreased bitumen production rates, while the amounts of steam produced through the tubing string increased. When the tubing string was extended past the heel of the well, bitumen production rates remained the same, but steam injection rates and SOR decreased. A lower pressure differential between the injector and producer wells was also observed. The study showed that SAGD producers can re-position production tubing strings in order to determine ratios of liquid production. It was concluded that although placing the short production tubing string close to the heel increased oil production, a longer tubing string improved production rates while lowering operating costs. 3 refs., 3 tabs., 35 figs.

  14. Optimization of Substrate Feeding for Enzymatic Biodiesel Production

    DEFF Research Database (Denmark)

    Price, Jason Anthony; Huusom, Jakob Kjøbsted; Nordblad, Mathias

    2013-01-01

    to be effective in mitigating the effects of substrate inhibition. Using enzymatic biodiesel production as a case study, the volumetric productivity of the reactor is increased while minimizing inactivation of the enzyme due to the alcohol. This is done by using a simple optimization routine where the substrate...... (both the vegetable oil and alcohol) feed rate/concentration is manipulated simultaneously. The results of the simulation were tested in the laboratory and are sufficiently positive to suggest the implementation of a feeding strategy for large scale enzymatic biodiesel production...

  15. Protocol optimization for enhanced production of pigments in Spirulina.

    Science.gov (United States)

    Kumar, Devendra; Kumar, Neeraj; Pabbi, Sunil; Walia, Suresh; Dhar, Dolly Wattal

    2013-01-01

    Spirulina has attracted special attention due to its importance as human foodstuff and natural colours with specific functional properties. These functional properties have been attributed to phycobilins, carotenoids, phenolics and unsaturated fatty acids. Present study was conducted under controlled phytotron conditions to identify the efficient strains of Spirulina in terms of pigment synthesis and to optimize their enhanced production. Methodology for enhanced production was standardized by varying specific environmental parameters (light intensity, temperature, carbon dioxide concentration, pH and NaCl level). Different strains of Spirulina depicted variability and environmental parameters showed distinct influence on pigments. Growth and pigment production was recorded to be most efficient under optimized conditions of light intensity (70 μmol m -2  s -1 ), temperature (30 °C), CO 2 concentration (550 ppm and 750 ppm), pH (10.5) and NaCl level (2 g L -1 ).

  16. Optimizing growth and conidia production of Cercospora medicaginis

    Directory of Open Access Journals (Sweden)

    Naceur DJEBALI

    2010-09-01

    Full Text Available The fungus Cercospora medicaginis is pathogenic to annual and perennial Medicago species. It grows slowly and produces only few conidia. To test the pathogenicity or virulence of C. medicaginis and to breed resistant lines of Medicago truncatula we optimized in vitro the growth and the conidium production of four isolates of C. medicaginis derived from M. truncatula and M. polymorpha. Of the eight media tested, that with wheat bran juice (WBJ yielded optimal growth and conidium production with most strains. The optimum growth temperature on WBJ medium was 25–30°C. Growth and conidia production were better in conditions of alternating light and darkness than with constant darkness. The best growth in the liquid WBJ medium occurred at pH 6–7, but the greatest number of conidia in that medium was obtained at pH 8–9.

  17. Naphthalene degradation and biosurfactant activity by Bacillus cereus 28BN

    Energy Technology Data Exchange (ETDEWEB)

    Tuleva, B.; Christova, N. [Inst. of Microbiology, Bulgarian Academy of Sciences, Sofia (Bulgaria); Jordanov, B.; Nikolova-Damyanova, B. [Inst. of Organic Chemistry, Sofia (Bulgaria); Petrov, P. [National Center of Infectious and Parasitic Diseases, Sofia (Bulgaria)

    2005-08-01

    Biosurfactant activity and naphthalene degradation by a new strain identified as Bacillus cereus 28BN were studied. The strain grew well and produced effective biosurfactants in the presence of n-alkanes, naphthalene, crude oil and vegetable oils. The biosurfactants were detected by the surface tension lowering of the medium, thin layer chromatography and infrared spectra analysis. With (2%) naphthalene as the sole carbon source, high levels of rhamnolipids at a concentration of 2.3 g l{sup -1} were determined in the stationary growth. After 20 d of incubation 72 {+-} 4% of the initial naphthalene was degraded. This is the first report for a Bacillus cereus rhamnolipid producing strain that utilized naphthalene under aerobic conditions. The strain looks promising for application in environmental technologies. (orig.)

  18. Biosurfactant-producing yeasts widely inhabit various vegetables and fruits.

    Science.gov (United States)

    Konishi, Masaaki; Maruoka, Naruyuki; Furuta, Yoshifumi; Morita, Tomotake; Fukuoka, Tokuma; Imura, Tomohiro; Kitamoto, Dai

    2014-01-01

    The isolation of biosurfactant-producing yeasts from food materials was accomplished. By a combination of a new drop collapse method and thin-layer chromatography, 48 strains were selected as glycolipid biosurfactant producers from 347 strains, which were randomly isolated from various vegetables and fruits. Of the producers, 69% were obtained from vegetables of the Brassica family. Of the 48 producers, 15 strains gave relatively high yields of mannosylerythritol lipids (MELs), and were identified as Pseudozyma yeasts. These strains produced MELs from olive oil at yields ranging from 8.5 to 24.3 g/L. The best yield coefficient reached 0.49 g/g as to the carbon sources added. Accordingly, MEL producers were isolated at high efficiency from various vegetables and fruits, indicating that biosurfactant producers are widely present in foods. The present results should facilitate their application in the food and related industries.

  19. Optimization of refinery product blending by using linear programming

    International Nuclear Information System (INIS)

    Ristikj, Julija; Tripcheva-Trajkovska, Loreta; Rikaloski, Ice; Markovska, Liljana

    1999-01-01

    The product slate of a simple refinery consists mainly of liquefied petroleum gas, leaded and unleaded gasoline, jet fuel, diesel fuel, extra light heating oil and fuel oil. The quality of the oil products (fuels) for sale has to comply with the adopted standards for liquid fuels, and the produced quantities have to be comply with the market needs. The oil products are manufactured by blending two or more different fractions which quantities and physical-chemical properties depend on the crude oil type, the way and conditions of processing, and at the same time the fractions are used to blend one or more products. It is in producer's interest to do the blending in an optimal way, namely, to satisfy the requirements for the oil products quality and quantity with a maximal usage of the available fractions and, of course, with a maximal profit out of the sold products. This could be accomplished by applying linear programming, that is by using a linear model for oil products blending optimization. (Author)

  20. Isolation of biosurfactant-producing marine bacteria and characteristics of selected biosurfactant

    Directory of Open Access Journals (Sweden)

    Kulnaree Phetrong

    2007-05-01

    Full Text Available Biosurfactant-producing marine bacteria were isolated from oil-spilled seawater collected from harbors and docks in Songkhla Province, Thailand. Haemolytic activity, emulsification activity toward nhexadecane,emulsion of weathered crude oil, drop collapsing test as well as oil displacement test were used to determine biosurfactant producing activity of marine bacteria. Among two-hundred different strains, 40strains exhibited clear zone on blood agar plates. Only eight strains had haemolytic activity and were able to emulsify weathered crude oil in marine broth during cultivation. Eight strains named SM1-SM8 wereidentified by 16S rRNA as Myroides sp. (SM1; Vibrio paraheamolyticus (SM2; Bacillus subtilis (SM3; Micrococcus luteus (SM4; Acinetobacter anitratus (SM6; Vibrio paraheamolyticus (SM7 and Bacilluspumilus (SM8. However, SM5 could not be identified. Strain SM1 showed the highest emulsification activity against weathered crude oil, by which the oil was emulsified within 24 h of cultivation. In addition, strainSM1 exhibited the highest activity for oil displacement test and emulsification test toward n-hexadecane. The emulsification activity against n-hexadecane of crude extract of strain SM1 was stable over a broadrange of temperature (30-121oC, pH (5-12 and salt concentration (0-9% NaCl, whereas CaCl2 showed an adverse effect on emulsifying activity.

  1. Glycolipid biosurfactants: main properties and potential applications in agriculture and food industry.

    Science.gov (United States)

    Mnif, Inès; Ghribi, Dhouha

    2016-10-01

    Glycolipids, consisting of a carbohydrate moiety linked to fatty acids, are microbial surface active compounds produced by various microorganisms. They are characterized by high structural diversity and have the ability to decrease the surface and interfacial tension at the surface and interface, respectively. Rhamnolipids, trehalolipids, mannosylerythritol lipids and cellobiose lipids are among the most popular glycolipids. They have received much practical attention as biopesticides for controlling plant diseases and protecting stored products. As a result of their antifungal activity towards phytopathogenic fungi and larvicidal and mosquitocidal potencies, glycolipid biosurfactants permit the preservation of plants and plant crops from pest invasion. Also, as a result of their emulsifying and antibacterial activities, glycolipids have great potential as food additives and food preservatives. Furthermore, the valorization of food byproducts via the production of glycolipid biosurfactant has received much attention because it permits the bioconversion of byproducts on valuable compounds and decreases the cost of production. Generally, the use of glycolipids in many fields requires their retention from fermentation media. Accordingly, different strategies have been developed to extract and purify glycolipids. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  2. Simultaneous valorization and biocatalytic upgrading of heavy vacuum gas oil by the biosurfactant-producing Pseudomonas aeruginosa AK6U.

    Science.gov (United States)

    Ismail, Wael Ahmed; Mohamed, Magdy El-Said; Awadh, Maysoon N; Obuekwe, Christian; El Nayal, Ashraf M

    2017-11-01

    Heavy vacuum gas oil (HVGO) is a complex and viscous hydrocarbon stream that is produced as the bottom side product from the vacuum distillation units in petroleum refineries. HVGO is conventionally treated with thermochemical process, which is costly and environmentally polluting. Here, we investigate two petroleum biotechnology applications, namely valorization and bioupgrading, as green approaches for valorization and upgrading of HVGO. The Pseudomonas aeruginosa AK6U strain grew on 20% v/v of HVGO as a sole carbon and sulfur source. It produced rhamnolipid biosurfactants in a growth-associated mode with a maximum crude biosurfactants yield of 10.1 g l -1 , which reduced the surface tension of the cell-free culture supernatant to 30.6 mN m -1 within 1 week of incubation. The rarely occurring dirhamnolipid Rha-Rha-C 12 -C 12 dominated the congeners' profile of the biosurfactants produced from HVGO. Heavy vacuum gas oil was recovered from the cultures and abiotic controls and the maltene fraction was extracted for further analysis. Fractional distillation (SimDist) of the biotreated maltene fraction showed a relative decrease in the high-boiling heavy fuel fraction (BP 426-565 °C) concomitant with increase in the lighter distillate diesel fraction (BP 315-426 °C). Analysis of the maltene fraction revealed compositional changes. The number-average (Mn) and weight-average (Mw) molecular weights, as well as the absolute number of hydrocarbons and sulfur heterocycles were higher in the biotreated maltene fraction of HVGO. These findings suggest that HVGO can be potentially exploited as a carbon-rich substrate for production of the high-value biosurfactants by P. aeruginosa AK6U and to concomitantly improve/upgrade its chemical composition. © 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

  3. Production and Supply Optimization in PT. Goodyear Indonesia Tbk

    Science.gov (United States)

    Edi Nugroho Soebandrija, Khristian; Reynaldo, Stephanus

    2013-06-01

    The Production and Supply Optimization in PT. Goodyear Indonesia Tbk and its study in this paper provide the purposes to create a model of the Master Production Schedule (MPS). The mentioned MPS complies with company policy, production capacity and supply received. From view point of Industrial Engineering, there are needs for forecasting method effectiveness toward the company production capacity. Furthermore, from view point of Information Systems, there are needs to provide the MPS as compare to prior systems that was done manually resulting in the frequent error at the time of making MPS deductible. Several Methods are used and considered, such as: The method of time series, Mean Square Error (MSE), Mean Absolute Percentage Error (MAPE), the calculation of production capacity, MPS, and designing information systems. By having the combination of views from Industrial Engineering and Information Systems perspective the improvement can be conducted from both theoretical development and its managerial implications.

  4. Production and Supply Optimization in PT. Goodyear Indonesia Tbk

    International Nuclear Information System (INIS)

    Soebandrija, Khristian Edi Nugroho; Reynaldo, Stephanus

    2013-01-01

    The Production and Supply Optimization in PT. Goodyear Indonesia Tbk and its study in this paper provide the purposes to create a model of the Master Production Schedule (MPS). The mentioned MPS complies with company policy, production capacity and supply received. From view point of Industrial Engineering, there are needs for forecasting method effectiveness toward the company production capacity. Furthermore, from view point of Information Systems, there are needs to provide the MPS as compare to prior systems that was done manually resulting in the frequent error at the time of making MPS deductible. Several Methods are used and considered, such as: The method of time series, Mean Square Error (MSE), Mean Absolute Percentage Error (MAPE), the calculation of production capacity, MPS, and designing information systems. By having the combination of views from Industrial Engineering and Information Systems perspective the improvement can be conducted from both theoretical development and its managerial implications.

  5. Determination of optimal conditions of oxytetracyclin production from streptomyces rimosus

    International Nuclear Information System (INIS)

    Zouaghi, Atef

    2007-01-01

    Streptomyces rimosus is an oxytetracycline (OTC) antibiotic producing bacteria that exhibited activities against gram positive and negative bacteria. OTC is used widely not only in medicine but also in production industry. The antibiotic production of streptomyces covers a very wide range of condition. However, antibiotic producers are particularly fastidious cultivated by proper selection of media such as carbon source. In present study we have optimised conditions of OTC production (Composition of production media, p H, shaking and temperature). The results have been shown that bran barley is the optimal media for OTC production at 28C pH5.8 at 150rpm for 5 days. For antibiotic determination, OTC was extracted with different organic solvent. Thin-layer chromatography system was used for separation and identification of OTC antibiotic. High performance liquid chromatographic (HPLC) method with ultraviolet detection for the analysis of OTC is applied to the determination of OTC purification. (Author). 24 refs

  6. Effects of Lactobacillus reuteri-derived biosurfactant on the gene expression profile of essential adhesion genes (gtfB, gtfC and ftf of Streptococcus mutans

    Directory of Open Access Journals (Sweden)

    Rasoul Salehi

    2014-01-01

    Full Text Available Background: Streptococci are the main causative agents in plaque formation and mutans streptococci are the principle etiological agent of dental plaque and caries. The process of biofilm formation is a step-wise process, starting with adhesion of planktonic cells to the surfaces. It is now a well known fact that expression of glucosyltransferases (gtfs and fructosyltransferase (ftf genes play a critical role in the initial adhesion of Streptococcus mutans to the tooth surface, which results in the formation of dental plaques and consequently caries and other periodontal diseases. Materials and Methods: In the present study, we have determined the effect of biosurfactants purified from Lactobacillus reuteri (DSM20016 culture on gene expression profile of gftB/C and fft of S. mutans (ATCC35668 using quantitative real-time polymerase chain reaction. Results: The application of biosurfactant caused considerable down-regulation of the expression of all three genes under study. The reduction in gene expression was statistically very significant (P > 0.0001 for all three genes. Conclusions: Inhibition of these genes by the extracted L. reuteri biosurfactant shows the emergence of a powerful alternative to the presently practicing alternatives. In view of the importance of these gene products for S. mutans attachment to the tooth surface, which is the initial important step in biofilm production and dental caries, we believe that the biosurfactant prepared in this study could be considered as a step ahead in dental caries prevention.

  7. Physiological and Molecular Characterization of Biosurfactant Producing Endophytic Fungi Xylaria regalis from the Cones of Thuja plicata as a Potent Plant Growth Promoter with Its Potential Application

    Directory of Open Access Journals (Sweden)

    Mohd Adnan

    2018-01-01

    Full Text Available Currently, there is an absolute concern for all nations in agricultural productivity to meet growing demands of human population. In recent time, biosurfactants produced by diverse group of microorganisms are used to achieve such demands as it is known for its ecofriendly use in elimination of plant pathogens and for increasing the bioavailability of nutrients for plants. Endophytic fungi are the important source of secondary metabolites and novel bioactive compounds for different biological applications. In the present study, endophytic fungi Xylaria regalis (X. regalis recovered from the cones of Thuja plicata was evaluated for its biosurfactant producing ability and plant growth-promoting abilities through various screening methods and also via its antagonistic activity against phytopathogens like Fusarium oxysporum and Aspergillus niger. In addition, X. regalis was also tested in vivo for a various range of growth parameters in chilli under greenhouse conditions. Significant increase in shoot and root length, dry matter production of shoot and root, chlorophyll, nitrogen, and phosphorus contents of chilli seedlings was found, which reveals its ability to improve the growth of crop plants. Hence, this study suggests the possibility of biosurfactant producing endophytic fungi X. regalis as a source of novel green biosurfactant for sustainable agriculture to achieve growing demands.

  8. Improved Ant Colony Optimization for Seafood Product Delivery Routing Problem

    Directory of Open Access Journals (Sweden)

    Baozhen Yao

    2014-02-01

    Full Text Available This paper deals with a real-life vehicle delivery routing problem, which is a seafood product delivery routing problem. Considering the features of the seafood product delivery routing problem, this paper formulated this problem as a multi-depot open vehicle routing problem. Since the multi-depot open vehicle routing problem is a very complex problem, a method is used to reduce the complexity of the problem by changing the multi-depot open vehicle routing problem into an open vehicle routing problem with a dummy central depot in this paper. Then, ant colony optimization is used to solve the problem. To improve the performance of the algorithm, crossover operation and some adaptive strategies are used. Finally, the computational results for the benchmark problems of the multi-depot vehicle routing problem indicate that the proposed ant colony optimization is an effective method to solve the multi-depot vehicle routing problem. Furthermore, the computation results of the seafood product delivery problem from Dalian, China also suggest that the proposed ant colony optimization is feasible to solve the seafood product delivery routing problem.

  9. Linear Optimization Techniques for Product-Mix of Paints Production in Nigeria

    Directory of Open Access Journals (Sweden)

    Sulaimon Olanrewaju Adebiyi

    2014-02-01

    Full Text Available Many paint producers in Nigeria do not lend themselves to flexible production process which is important for them to manage the use of resources for effective optimal production. These goals can be achieved through the application of optimization models in their resources allocation and utilisation. This research focuses on linear optimization for achieving product- mix optimization in terms of the product identification and the right quantity in paint production in Nigeria for better profit and optimum firm performance. The computational experiments in this research contains data and information on the units item costs, unit contribution margin, maximum resources capacity, individual products absorption rate and other constraints that are particular to each of the five products produced in the company employed as case study. In data analysis, linear programming model was employed with the aid LINDO 11 software to analyse the data. The result has showed that only two out of the five products under consideration are profitable. It also revealed the rate to which the company needs to reduce cost incurred on the three other products before making them profitable for production.

  10. Validation of optimization strategies using the linear structured production chains

    Science.gov (United States)

    Kusiak, Jan; Morkisz, Paweł; Oprocha, Piotr; Pietrucha, Wojciech; Sztangret, Łukasz

    2017-06-01

    Different optimization strategies applied to sequence of several stages of production chains were validated in this paper. Two benchmark problems described by ordinary differential equations (ODEs) were considered. A water tank and a passive CR-RC filter were used as the exemplary objects described by the first and the second order differential equations, respectively. Considered in the work optimization problems serve as the validators of strategies elaborated by the Authors. However, the main goal of research is selection of the best strategy for optimization of two real metallurgical processes which will be investigated in an on-going projects. The first problem will be the oxidizing roasting process of zinc sulphide concentrate where the sulphur from the input concentrate should be eliminated and the minimal concentration of sulphide sulphur in the roasted products has to be achieved. Second problem will be the lead refining process consisting of three stages: roasting to the oxide, oxide reduction to metal and the oxidizing refining. Strategies, which appear the most effective in considered benchmark problems will be candidates for optimization of the mentioned above industrial processes.

  11. Optimal subsidy policy for accelerating the diffusion of green products

    Directory of Open Access Journals (Sweden)

    Hongguang Peng

    2013-06-01

    Full Text Available Purpose: We consider a dynamic duopoly market in which two firms respectively produce green products and conventional products. The two types of product can substitute each other in some degree. Their demand rates depend on not only prices but the consumers’ increasing environmental awareness. Too high initial cost relative to conventional products becomes one of the major obstacles that hinder the adoption of green products. The government employs subsidy policy to trigger the adoption of green products. The purpose of the paper is to explore the optimal subsidy strategy to fulfill the government’s objective. Design/methodology/approach: We suppose the players in the game employ open-loop strategies, which make sense since the government generally cannot alter his policy for political and economic purposes. We take a differential game approach and use backward induction to analyze the firms’ pricing strategy under Cournot competition, and then focus upon a Stackelberg equilibrium to find the optimal subsidy strategy of the government. Findings: The results show that the more remarkable the energy or environmental performance, or the bigger the initial cost of green products, the higher the subsidy level should be. Due to the increasing environmental awareness and the learning curve, the optimal subsidy level decreases over time. Research limitations/implications: In our model several simplifying assumptions are made to keep the analysis more tractable. In particular, we have assumed only one type of green product. In reality several types of product with different energy or environmental performances exist. Our research can be extended in future work to take into account product differentiation on energy or environmental performance and devise a discriminatory subsidy policy accordingly. Originality/value: In the paper we set the objective of the government as minimizing the total social cost induced by the energy consumption or

  12. Optimizing Growth Conditions for Digoxin Production in Digitalis lanata Ehrh

    Directory of Open Access Journals (Sweden)

    Herman A. van Wietmarschen

    2016-08-01

    Full Text Available Objective: Digoxin is a therapeutic cardenolide widely used to treat various heart conditions such as atrial flutter, atrial fibrillation and heart failure in both Western as well as Chinese medicine. Digoxin is extracted from cultivated Digitalis lanata Ehrh. plants, known as Mao Hua Yang Di Huang in Chinese medicine. This manuscript presents two studies that were conducted to optimize the cultivation conditions for digoxin production in the TCM Mao Hua Yan Di Huang in a greenhouse under GAP conditions.

  13. Profit and Risk Measures in Oil Production Optimization

    DEFF Research Database (Denmark)

    Capolei, Andrea; Foss, Bjarne; Jørgensen, John Bagterp

    2015-01-01

    In oil production optimization, we usually aim to maximize a deterministic scalar performance index such as the profit over the expected reservoir lifespan. However, when uncertainty in the parameters is considered, the profit results in a random variable that can assume a range of values dependi...... pro and cons for each of them. Finally, among the presented risk measures, we identify two of them as appropriate risk measures when minimizing the risk....

  14. Optimization of neutron flux distribution in Isotope Production Reactor

    International Nuclear Information System (INIS)

    Valladares, G.L.

    1988-01-01

    In order to optimize the thermal neutrons flux distribution in a Radioisotope Production and Research Reactor, the influence of two reactor parameters was studied, namely the Vmod / Vcomb ratio and the core volume. The reactor core is built with uranium oxide pellets (UO 2 ) mounted in rod clusters, with an enrichment level of ∼3 %, similar to LIGHT WATER POWER REATOR (LWR) fuel elements. (author) [pt

  15. Optimizing Greenhouse Rice Production: What Is the Best Pot Size?

    OpenAIRE

    Eddy, Robert; Acosta, Kevin; Liu, Yisi; Russell, Michael

    2016-01-01

    This publication describes our studies to determine the best pot size to optimize greenhouse rice production. We recommend 9-cm (4-inch) diameter square pot. Pots as small as 7-cm diameter yielded seed. This version is updated to include observations of larger pots with multiple plants. Photos of the plants growing under differing pot sizes are provided. This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & L...

  16. Optimization of Protein Hydrolysate Production Process from Jatropha curcas Cake

    OpenAIRE

    Waraporn Apiwatanapiwat; Pilanee Vaithanomsat; Phanu Somkliang; Taweesiri Malapant

    2009-01-01

    This was the first document revealing the investigation of protein hydrolysate production optimization from J. curcas cake. Proximate analysis of raw material showed 18.98% protein, 5.31% ash, 8.52% moisture and 12.18% lipid. The appropriate protein hydrolysate production process began with grinding the J. curcas cake into small pieces. Then it was suspended in 2.5% sodium hydroxide solution with ratio between solution/ J. curcas cake at 80:1 (v/w). The hydrolysis reactio...

  17. Cost Optimization of Product Families using Analytic Cost Models

    DEFF Research Database (Denmark)

    Brunø, Thomas Ditlev; Nielsen, Peter

    2012-01-01

    This paper presents a new method for analysing the cost structure of a mass customized product family. The method uses linear regression and backwards selection to reduce the complexity of a data set describing a number of historical product configurations and incurred costs. By reducing the data...... set, the configuration variables which best describe the variation in product costs are identified. The method is tested using data from a Danish manufacturing company and the results indicate that the method is able to identify the most critical configuration variables. The method can be applied...... in product family redesign projects focusing on cost reduction to identify which modules contribute the most to cost variation and should thus be optimized....

  18. Optimization of fermentation conditions for ethanol production from whey

    Energy Technology Data Exchange (ETDEWEB)

    Castillo, F J; Izaguirre, M F; Michelena, V; Moreno, B

    1982-01-01

    Optimal conditions for ethanol production in 7% whey solutions by the yeast Candida pseudotropicalis ATCC 8619 included an initial pH of 4.57 and 30 degrees. Complete fermentation of the available lactose took place without supplementary nutrients; additions of N and P salts, yeast extract, or corn steep liquor resulted in increased yeast production and lower ethanol yields. A possible correlation was observed between increases in yeast inocula and lactose utilization and ethanol production rates; 8.35 g ethanol/L was obtained within 22 hours by using a yeast inoculum of 13.9 g/L. No differences in fermentation rates or ethanol yields were observed when whole or deproteinized whey solutions were used. Concentrated whey permeates, obtained after removal of the valuable proteins from whey, can be effectively fermented for ethanol production.

  19. Probabilistic framework for product design optimization and risk management

    Science.gov (United States)

    Keski-Rahkonen, J. K.

    2018-05-01

    Probabilistic methods have gradually gained ground within engineering practices but currently it is still the industry standard to use deterministic safety margin approaches to dimensioning components and qualitative methods to manage product risks. These methods are suitable for baseline design work but quantitative risk management and product reliability optimization require more advanced predictive approaches. Ample research has been published on how to predict failure probabilities for mechanical components and furthermore to optimize reliability through life cycle cost analysis. This paper reviews the literature for existing methods and tries to harness their best features and simplify the process to be applicable in practical engineering work. Recommended process applies Monte Carlo method on top of load-resistance models to estimate failure probabilities. Furthermore, it adds on existing literature by introducing a practical framework to use probabilistic models in quantitative risk management and product life cycle costs optimization. The main focus is on mechanical failure modes due to the well-developed methods used to predict these types of failures. However, the same framework can be applied on any type of failure mode as long as predictive models can be developed.

  20. Biosurfactant-and-bioemulsifier produced by a promising Cunninghamella echinulata isolated from Caatinga soil in the northeast of Brazil.

    Science.gov (United States)

    Andrade Silva, Nadielly R; Luna, Marcos A C; Santiago, André L C M A; Franco, Luciana O; Silva, Grayce K B; de Souza, Patrícia M; Okada, Kaoru; Albuquerque, Clarissa D C; da Silva, Carlos A Alves; Campos-Takaki, Galba M

    2014-09-01

    A Mucoralean fungus was isolated from Caatinga soil of Pernambuco, Northeast of Brazil, and was identified as Cunninghamella echinulata by morphological, physiological, and biochemical tests. This strain was evaluated for biosurfactant/bioemulsifier production using soybean oil waste (SOW) and corn steep liquor (CSL) as substrates, added to basic saline solution, by measuring surface tension and emulsifier index and activity. The best results showed the surface water tension was reduced from 72 to 36 mN/m, and an emulsification index (E₂₄) of 80% was obtained using engine oil and burnt engine oil, respectively. A new molecule of biosurfactant showed an anionic charge and a polymeric chemical composition consisting of lipids (40.0% w/w), carbohydrates (35.2% w/w) and protein (20.3% w/w). In addition, the biosurfactant solution (1%) demonstrated its ability for an oil displacement area (ODA) of 37.36 cm², which is quite similar to that for Triton X-100 (38.46 cm²). The stability of the reduction in the surface water tension as well as of the emulsifier index proved to be stable over a wide range of temperatures, in pH, and in salt concentration (4%-6% w/v). The biosurfactant showed an ability to reduce and increase the viscosity of hydrophobic substrates and their molecules, suggesting that it is a suitable candidate for mediated enhanced oil recovery. At the same time, these studies indicate that renewable, relatively inexpensive and easily available resources can be used for important biotechnological processes.