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Sample records for onion maggot delia

  1. Effects of fall and spring seeding date and other agronomic factors on infestations of root maggots, Delia spp. (Diptera: Anthomyiidae), in canola.

    Science.gov (United States)

    Dosdall, L M; Clayton, G W; Harker, K N; O'Donovan, J T; Stevenson, F C

    2006-10-01

    Several agronomic benefits can result from fall seeding of canola (Brassica spp.), but extensive research data are lacking on the potential impact of this practice on infestations of root maggots (Delia spp.) (Diptera: Anthomyiidae), which are major pests of the crop in western Canada. Field experiments making up 13 location by year combinations were conducted in central Alberta, Canada, from 1998 to 2001 to determine the effect of fall versus spring seeding of canola on root maggot damage. Depending on the experiment, interactions with seeding rate, seed treatment, timing of weed removal, and canola species (cultivar) also were investigated. Root maggot damage declined with an increase in seeding rate for plots seeded in May but not in fall or April. Susceptibility to infestation was greater for plants of Brassica rapa L. than Brassica napus L., but seed treatment had no effect on damage by these pests. Combined analysis using data from all experiment by location by year combinations indicated that seeding date had no significant effect on root maggot damage. The extended emergence of Delia spp. adults, which spans the appearance of crop stages vulnerable to oviposition regardless of seeding date, prevented reduced root maggot attack. Covariance analysis demonstrated the importance of increasing seeding rate for reducing root maggot infestations, a practice that can be especially beneficial for May-seeded canola when growing conditions limit the ability of plants to compensate for root maggot damage. Results determined with the small plot studies described here should be validated in larger plots or on a commercial field scale, but both the combined and covariance analyses indicate that seeding canola in fall does not predispose plants to greater damage by larval root maggots than seeding in spring.

  2. Laboratory studies of radiation-induced sterility on the onion maggot, delia antiqua (diptera: anthomyiidae)

    International Nuclear Information System (INIS)

    McEwen, F.L.; Ritchey, G.; Liu, H.J.

    1984-01-01

    A dose of 4 krad was the most appropriate for sterilizing pupae of Delia antiqua (Meigen) for field release: adult emergence was not reduced, emerging female flies were sterile, and the eggs produced by normal females which had mated with males irradiated as pupae had a lower hatch than those resulting from matings with normal males. Males irradiated as 6- to 8-day-old pupae were fully competitive under laboratory conditions. Continuous scrutiny of laboratory cultures to detect reduction in adult emergence or reduced competitiveness of males is stressed

  3. The de novo transcriptome and its analysis in the worldwide vegetable pest, Delia antiqua (Diptera: Anthomyiidae).

    Science.gov (United States)

    Zhang, Yu-Juan; Hao, Youjin; Si, Fengling; Ren, Shuang; Hu, Ganyu; Shen, Li; Chen, Bin

    2014-03-10

    The onion maggot Delia antiqua is a major insect pest of cultivated vegetables, especially the onion, and a good model to investigate the molecular mechanisms of diapause. To better understand the biology and diapause mechanism of the insect pest species, D. antiqua, the transcriptome was sequenced using Illumina paired-end sequencing technology. Approximately 54 million reads were obtained, trimmed, and assembled into 29,659 unigenes, with an average length of 607 bp and an N50 of 818 bp. Among these unigenes, 21,605 (72.8%) were annotated in the public databases. All unigenes were then compared against Drosophila melanogaster and Anopheles gambiae. Codon usage bias was analyzed and 332 simple sequence repeats (SSRs) were detected in this organism. These data represent the most comprehensive transcriptomic resource currently available for D. antiqua and will facilitate the study of genetics, genomics, diapause, and further pest control of D. antiqua. Copyright © 2014 Zhang et al.

  4. Improving the biocontrol potential of Steinernema feltiae against Delia radicum through dosage, application technique and timing.

    Science.gov (United States)

    Beck, Bert; Spanoghe, Pieter; Moens, Maurice; Brusselman, Eva; Temmerman, Femke; Pollet, Sabien; Nuyttens, David

    2014-05-01

    The potential of the entomopathogenic nematode (EPN) Steinernema feltiae Filipjev as a biocontrol agent against the cabbage maggot Delia radicum (L.), was assessed in three field tests, focusing on EPN dosage, application technique and timing. Spraying cabbage plant trays with different doses of infective juveniles (IJs) (50,000, 100,000 and 200,000 per plant) generated a similar reduction of plant mortality. Spraying plant trays with 200,000 IJs of Steinernema feltiae per plant temporarily reduced the number of maggots around the plants' roots, while neither spraying a lower dose (50,000 IJs/plant) nor soil drenching with 200,000 or 50,000 IJs/plant) reduced maggot numbers. When applied as a plant tray spray, IJs of S. feltiae took 1-2 weeks to spread through the soil surrounding the roots. The pathogenicity of the EPNs, as evaluated by a Galleria mellonella bait test, was highest (up to 100% mortality) until up to five weeks after application, and declined to control levels after 4-7 weeks. Follow-up drench applications with EPNs, applied one and/or two weeks after the first EPN application, did not influence control of Delia radicum. Plant tray spraying provides better placement of Steinernema feltiae than soil drench treatments for control of Delia radicum. Plant mortality was not dose-dependent in the presented trials, unlike the reduction of maggot numbers. Further research into timing and application technique of follow-up treatments with S. feltiae is required to increase efficacy to commercial standards. © 2013 Society of Chemical Industry.

  5. Repressed Beauveria bassiana Infections in Delia antiqua due to Associated Microbiota.

    Science.gov (United States)

    Zhou, Fangyuan; Wu, Xiaoqing; Xu, Letian; Guo, Shuhai; Chen, Guanhong; Zhang, Xinjian

    2018-05-23

    Insects form both mutualistic and antagonistic relationships with microbes, and some antagonistic microbes have been used as biocontrol agents (BCAs) in pest management. Contextually, BCAs may be inhibited by beneficial insect symbionts, which can become potential barriers for entomopathogen-dependent pest biocontrol. Thus, by using the symbioses formed by one devastating dipteran pest, Delia antiqua, and its associated microbes as a model system, we sought to determine whether the antagonistic interaction between BCAs and microbial symbionts could affect the outcome of entomopathogen-dependent pest biocontrol. The result showed that in contrast to non-axenic D. antiqua larvae, i.e., onion maggots, axenic larvae lost resistance to the entomopathogenic Beauveria bassiana, and the re-inoculation of microbiota increased the resistance of axenic larvae to B. bassiana. Furthermore, bacteria, including Citrobacter freundii, Enterobacter ludwigii, Pseudomonas protegens, Serratia plymuthica, Sphingobacterium faecium, and Stenotrophomonas maltophilia, frequently isolated from larvae suppressed B. bassiana conidia germination and hyphal growth, and the re-inoculation of specific individual bacteria enhanced the resistance of axenic larvae to B. bassiana. Bacteria associated with larvae, including C. freundii, E. ludwigii, P. protegens, S. plymuthica, S. faecium, and S. maltophilia, can inhibit B. bassiana infection. Removing the microbiota can suppress larval resistance to fungal infection. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  6. The onion fly

    International Nuclear Information System (INIS)

    Loosjes, M.

    1990-01-01

    This paper describes the origin, practical application, problems in application and prospects of control of the onion fly, Delia antiqua (Diptera: Anthomyiidae), in the Netherlands by the Sterile Insect Technique (SIT). The larva of the onion fly is a severe pest in onions in temperate regions. Development of resistance of the onion fly against insecticides caused research on the SIT to be started by the Dutch Government in 1965. This research was on mass-rearing, long-term storage of pupae, sterilization, and release and ratio assessment techniques. By 1979 sufficient information had been turned over to any interested private company. In the case of the onion fly the SIT can be applied like a control treatment instead of chemical control to individual onion fields. This is due to the limited dispersal activity of the flies and the scattered distribution of onion fields in the Netherlands, with 5-10% of the onion growing areas planted with onions

  7. Bioassay and Scanning Electron Microscopic Observations Reveal High Virulence of Entomopathogenic Fungus, Beauveria bassiana, on the Onion Maggot (Diptera: Anthomyiidae) Adults.

    Science.gov (United States)

    Zhang, Hui; Wu, Shengyong; Xing, Zhenlong; Wang, Xiaoqing; Lei, Zhongren

    2016-12-01

    When flies were dipped in 1 × 10 8 conidia/ml conidia suspensions and then kept in the incubator (22 ± 1 °C, 70 ± 5% RH), scanning electron microscope observations revealed that, at 2 h, the majority of adhering Beauveria bassiana conidia were attached to either the wing surface or the interstitial area between the macrochaetae on the thorax and abdomen of the onion maggot adults. Germ tubes were being produced and had oriented toward the cuticle by 18 h. Penetration of the insect cuticle had occurred by 36 h, and by 48 h, germ tubes had completely penetrated the cuticle. Fungal mycelia had emerged from the insect body and were proliferating after 72 h. The superficial area and structure of the wings and macrochaetae may facilitate the attachment of conidia and enable effective penetration. The susceptibility of adults to 12 isolates, at a concentration of 1 × 10 7 conidia/ml, was tested in laboratory experiments. Eight of the more potent strains caused in excess of 85% adult mortality 8 d post inoculation, while the median lethal time (LT 50 ) of these strains was bassiana strains are highly virulent to onion maggot adults and should be considered as potential biocontrol agents against the adult flies. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  8. Maggot Therapy: The Science and Implication for CAM Part II—Maggots Combat Infection

    Directory of Open Access Journals (Sweden)

    Yamni Nigam

    2006-01-01

    Full Text Available Maggot therapy employs the use of freshly emerged, sterile larvae of the common green-bottle fly, Phaenicia (Lucilia sericata, and is a form of artificially induced myiasis in a controlled clinical situation. Maggot therapy has the following three core beneficial effects on a wound: debridement, disinfection and enhanced healing. In part II of this review article, we discuss clinical infections and the evidence supporting the potent antibacterial action of maggot secretions. Enhancement of wound healing by maggots is discussed along with the future of this highly successful, often controversial, alternative treatment.

  9. Growth and Survival of Bagged Lucilia sericata Maggots in Wounds of Patients Undergoing Maggot Debridement Therapy

    Directory of Open Access Journals (Sweden)

    Helena Čičková

    2013-01-01

    Full Text Available Maggot debridement therapy (MDT is an established method of debridement of nonhealing wounds. Despite intense clinical research about its efficacy and effects of substances produced by the larvae, growth and development of maggots in the wounds remain largely unexplored. In the present study, the bags with larvae (n=52, which had been used to debride traumatic, ischemic, diabetic and venous ulcers, were collected and examined. Survival, length, width and larval instar of the maggots within each bag were recorded and analyzed with respect to the wound type and duration of the treatment. Survival of maggots after a 48-h cycle of MDT ranged between 63.6 and 82.7%. Maggots in venous ulcers had on average 9–19% higher mortality than maggots within traumatic, ischemic, and diabetic ulcers. Length of larvae after 48 h cycle of MDT reached on average 7.09–9.68 mm, and average width varied between 1.77 and 2.26 mm. Larvae in venous ulcers were significantly smaller after 48 h, but not after 72 h treatment compared to the other wound types. Further studies should be aimed to identify other patient-associated factors which might influence growth and survival of the larvae during maggot debridement therapy.

  10. Growth and Survival of Bagged Lucilia sericata Maggots in Wounds of Patients Undergoing Maggot Debridement Therapy.

    Science.gov (United States)

    Cičková, Helena; Cambal, Marek; Kozánek, Milan; Takáč, Peter

    2013-01-01

    Maggot debridement therapy (MDT) is an established method of debridement of nonhealing wounds. Despite intense clinical research about its efficacy and effects of substances produced by the larvae, growth and development of maggots in the wounds remain largely unexplored. In the present study, the bags with larvae (n = 52), which had been used to debride traumatic, ischemic, diabetic and venous ulcers, were collected and examined. Survival, length, width and larval instar of the maggots within each bag were recorded and analyzed with respect to the wound type and duration of the treatment. Survival of maggots after a 48-h cycle of MDT ranged between 63.6 and 82.7%. Maggots in venous ulcers had on average 9-19% higher mortality than maggots within traumatic, ischemic, and diabetic ulcers. Length of larvae after 48 h cycle of MDT reached on average 7.09-9.68 mm, and average width varied between 1.77 and 2.26 mm. Larvae in venous ulcers were significantly smaller after 48 h, but not after 72 h treatment compared to the other wound types. Further studies should be aimed to identify other patient-associated factors which might influence growth and survival of the larvae during maggot debridement therapy.

  11. DELIA DOMÍNGUEZ: EXPERIENCIA Y CANTO

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    Ana María Cuneo M.

    2004-04-01

    Full Text Available La presente reflexión tiene por objeto configurar la "poética" inscrita en los textos poéticos de Delia Domínguez para develar el modo como la autora concibe su propio quehacer. La observación se ha realizado respetando el orden diacrónico para hacer visible y registrar el desarrollo escrituralThe present reflexion proposes to configurate the "poetics" inscribed in the poetical wor5k of Delia Domínguez, in order to reveal the way the author conceives her own craft. This observation is made in accord to the diachronic order which makes visible and permits to follow the writing process

  12. POTENSI MAGGOT UNTUK PENINGKATAN PERTUMBUHAN DAN STATUS KESEHATAN IKAN

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    Melta Rini Fahmi

    2016-11-01

    Full Text Available Penggunaan maggot sebagai pakan alternatif ikan telah dikaji di Loka Riset Budidaya Ikan Hias Air Tawar (LRBIHAT, Depok. Maggot merupakan larva serangga black soldier (Hermetia illusence yang dapat mengkonversi material organik menjadi biomassanya. Salah satu keunggulan maggot adalah dapat diproduksi dalam berbagai ukuran, sesuai dengan kebutuhan. Penyimpanan maggot pada suhu rendah dapat menghambat pertumbuhan dan mempertahankan kehidupannya. Produksi maggot pada ukuran kecil dimulai dari penyediaan telur, penetasan, dan pembesaran dalam media PKM (Palm Kernel Meal atau bungkil kelapa sawit, pemanenan dan penyimpanan dalam suhu rendah. Nilai nutrisi maggot pada umur 6-7 hari adalah protein: 60,2%; lemak: 13,3%; abu: 7,7%; karbohidrat: 18,8%. Percobaan pemanfaatan maggot sebagai suplemen pakan diujikan terhadap ikan Balashark (Balantiocheilus melanopterus Bleeker ukuran 2,0 ± 0,2 g. Hasil yang didapatkan menunjukkan bahwa pemberian maggot memberikan pertumbuhan dan sintasan yang lebih baik. Dampak penambahan maggot pada ikan terlihat signifikan terhadap gambaran darah ikan yang menunjukkan daya tahan tubuh ikan yang lebih baik. Maggot utilization as fish feed alternative has been studied at Loka Riset Budidaya Ikan Hias Air Tawar (LRBIHAT, Depok. Maggot is an insect larvae of black soldier (Hermetia illusence that can convert organic material to its body biomass. One of the advantages in maggot culture is that it can be produced in different sizes according to fish requirement. Keeping maggot at low temperature can delay its growth while keeping it alive. Production of small size maggot starts from eggs preparation, hatching, and rearing in media of PKM (Palm Kernel Meal or coconut oil cake of palm, cropping and then keeping it in low temperature. Nutritional value of maggot at the age of 6-7 days is as follows: protein, 60.2%, fat; 13.3%, ash; 7.7%, carbohydrate; 18.8%. Trial feeding using maggot as feed supplement was done on Balashark

  13. Influence of Interval Between Postharvest Lettuce Residue Management and Subsequent Seeding of Broccoli on Cabbage Maggot (Diptera: Anthomyiidae) Infestation on Broccoli.

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    Joseph, Shimat V; Godfrey, Larry D; Bettiga, Christopher

    2017-10-01

    Larval stages of cabbage maggot, Delia radicum (L.) (Diptera: Anthomyiidae), attack the roots of Brassica crops and cause severe economic damage. In the Salinas Valley of California, Brassica crops are often planted after successive lettuce (Lactuca sativa L.) crops. The interval between postharvest soil incorporation of lettuce residue and the subsequent Brassica crop can be as short as 7 d, which could influence D. radicum infestation on broccoli (Brassica oleracea var. italica Plenck). In 2014 and 2015, the effect of intervals between crops (IBC) on D. radicum infestation was evaluated. The treatments were 7, 20, 33, and 48 d IBC, and NL (no lettuce), 7, 21, 36, and 49 d IBC in 2014 and 2015, respectively. Insect counts and feeding damage on broccoli was assessed during 3-6 wk after planting. Adult Delia fly captures were significantly greater at 7 d than 36-49 d IBC in both years. In both years, D. radicum eggs collected were significantly greater at 7 d than at 33 d or 36 d IBC plots. Larvae collected were significantly greater at 7 d IBC than all other treatments in 2014, but not in 2015. Similarly, severity of feeding injury was significantly greater in 7 d than 33 d or 48 d IBC in 2014, but not in 2015. In 2015, broccoli with no prior lettuce had significantly lower Delia flies and D. radicum egg densities than 7 d or 21 d IBC. The implication of these results as a cultural control tactic for D. radicum infestation is discussed. © The Author 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  14. Growth and Survival of Bagged Lucilia sericata Maggots in Wounds of Patients Undergoing Maggot Debridement Therapy

    OpenAIRE

    Čičková, Helena; Čambal, Marek; Kozánek, Milan; Takáč, Peter

    2013-01-01

    Maggot debridement therapy (MDT) is an established method of debridement of nonhealing wounds. Despite intense clinical research about its efficacy and effects of substances produced by the larvae, growth and development of maggots in the wounds remain largely unexplored. In the present study, the bags with larvae (n = 52), which had been used to debride traumatic, ischemic, diabetic and venous ulcers, were collected and examined. Survival, length, width and larval instar of the maggots withi...

  15. Tracing heavy metals in 'swine manure - maggot - chicken' production chain.

    Science.gov (United States)

    Wang, Wanqiang; Zhang, Wenjuan; Wang, Xiaoping; Lei, Chaoliang; Tang, Rui; Zhang, Feng; Yang, Qizhi; Zhu, Fen

    2017-08-21

    With the development of large-scale livestock farming, manure pollution has drawn much attention. Conversion by insects is a rapid and cost-effective new method for manure management. Swine manure conversion with maggots (Musca domestica larvae) has developed, and the harvested maggots are often used as animal feed. However, the flow of heavy metals from manure to downstream processes cannot be ignored, and therefore, heavy metal content was measured in untreated raw manure, maggot-treated manure, harvested maggots and maggot-eating chickens (chest muscle and liver) to evaluate potential heavy metal risks. The levels of zinc, copper, chromium, selenium, cadmium and lead had significant differences between untreated raw manure and maggot-treated manure. The concentrations of all detected heavy metals, except for cadmium and selenium, in maggots met the limits established by the feed or feed additive standards of many countries. The bioaccumulation factor (BAF) of heavy metals decreased with the increase of the maggot instar, indicating that heavy metals were discharged from the bodies of maggots with the growth of maggots. Also, the contents of overall heavy metals in chickens fed harvested maggots met the standards for food. In conclusion, regarding heavy metals, it is eco-safe to use maggots in manure management.

  16. Maggot therapy´s modes of action : effect of maggot secretions on microbiological, haematological and immunological processes

    NARCIS (Netherlands)

    Plas, Maartje Jeriena Adriana van der

    2009-01-01

    The work described in this thesis focussed on the modes of action of maggot therapy in chronic wounds, especially related to the inflammatory phase of wound healing. For this purpose, the effect of maggot excretions and/or secretions on microbiological, haematological and immunological processes was

  17. Maggot debridement

    DEFF Research Database (Denmark)

    Gottrup, Finn; Jørgensen, Bo

    2011-01-01

    Debridement is an essential component to promote healing in a problem wound. Several techniques are available including maggot debridement therapy (MDT). Objective: To describe the efficacy of MDT for treating problem wound especially diabetic foot ulcers. Methods: The topic is elucidated from...... different points of view: the mode of action, when to use, use in a practice, clinical results, and discussing the problem of creating evidence for the clinical effect. Results: Literature and own results demonstrate that MDT is a safe method with few side effects. Maggot debridement therapy is as good...... as or better than conventional often surgical debridement, is more selective than surgical debridement, decreases time to healing and stay of patients in the ward, and may decrease the risk of major amputations. However, the evidence of these effects of MDT on the highest level is presently lacking. A detailed...

  18. Housefly maggot-treated composting as sustainable option for pig manure management.

    Science.gov (United States)

    Zhu, Feng-Xiang; Yao, Yan-Lai; Wang, Su-Juan; Du, Rong-Guang; Wang, Wei-Ping; Chen, Xiao-Yang; Hong, Chun-Lai; Qi, Bing; Xue, Zhi-Yong; Yang, Hong-Quan

    2015-01-01

    In traditional composting, large amounts of bulking agents must be added to reduce the moisture of pig manure, which increases the cost of composting and dilutes the N, P and K content in organic fertilizers. In this study, maggot treatment was used in composting instead of bulking agents. In experiment of selecting an optimal inoculum level for composting, the treatment of 0.5% maggot inoculum resulted in the maximum yield of late instar maggots, 11.6% (maggots weight/manure weight). The manure residue became noticeably granular by day 6 and its moisture content was below 60%, which was suitable for further composting without bulking agents. Moreover, in composting experiment with a natural compost without maggot inoculum and maggot-treated compost at 0.5% inoculum level, there were no significant differences in nutrient content between the two organic fertilizers from the two treatments (paired Student's t15=1.0032, P=0.3317). Therefore, maggot culturing did not affect the characteristics of the organic fertilizer. The content of TNPK (total nitrogen+total phosphorus+total potassium) in organic fertilizer from maggot treatment was 10.72% (dry weight), which was far more than that of organic fertilizer made by conventional composting with bulking agents (about 8.0%). Dried maggots as feed meet the national standard (GB/T19164-2003) for commercial fish meal in China, which contained 55.32 ± 1.09% protein; 1.34 ± 0.02% methionine; 4.15 ± 0.10% lysine. This study highlights housefly maggot-treated composting can be considered sustainable alternatives for pig manure management to achieve high-quality organic fertilizer and maggots as feed without bulking agents. Copyright © 2014 Elsevier Ltd. All rights reserved.

  19. Yellow and the Novel Aposematic Signal, Red, Protect Delias Butterflies from Predators

    OpenAIRE

    Wee, Jocelyn Liang Qi; Monteiro, Ant?nia

    2017-01-01

    Butterflies of the South Asian and Australian genus Delias possess striking colours on the ventral wings that are presumed to serve as warning signals to predators. However, this has not been shown empirically. Here we experimentally tested whether the colours of one member of this diverse genus, Delias hyparete, function as aposematic signals. We constructed artificial paper models with either a faithful colour representation of D. hyparete, or with all of its colours converted to grey scale...

  20. [Maggot therapy for gangrene and osteomyelitis].

    Science.gov (United States)

    Mumcuoglu, K Y; Lipo, M; Ioffe-Uspensky, I; Miller, J; Galun, R

    1997-03-02

    5 patients with diabetic-foot were treated by maggot therapy. The most serious case was in a 75-year-old man who had gangrene and osteomyelitis of the right foot. Proteus mirabilis, Enterococcus sp., Providencia stuartii and Staphylococcus spec. (coagulase positive) were isolated from lesions which did not respond to antibiotic therapy. The patient had twice refused amputation but agreed to maggot therapy. Larvae of the sheep blowfly Phoenicia (Lucilia) sericata were used for twice-weekly treatment over a period of 7 months. Sterile larvae were applied to the wound and replaced every 3-4 days. After 4 months of treatment, the necrotic tissue around the toes and on the sole of the foot detached from the healthy tissue. During the last 3 months of treatment the larvae removed the remaining infected tissue. As therapy progressed, new layers of healthy tissue covered the wound. The offensive odor associated with the necrotic tissue and the intense pain in the foot decreased significantly. At the end of therapy, during which there were no complaints of discomfort, he was able to walk. In the 4 other patients who had relatively superficial gangrene, the maggots debrided the wounds within 2-4 weeks. Thereafter treatment was continued with antibiotics. Maggot therapy can be recommended in cases of intractable gangrene and osteomyelitis, when treatment with antibiotics and surgical debridement have failed.

  1. Maggot excretions inhibit biofilm formation on biomaterials.

    Science.gov (United States)

    Cazander, Gwendolyn; van de Veerdonk, Mariëlle C; Vandenbroucke-Grauls, Christina M J E; Schreurs, Marco W J; Jukema, Gerrolt N

    2010-10-01

    Biofilm-associated infections in trauma surgery are difficult to treat with conventional therapies. Therefore, it is important to develop new treatment modalities. Maggots in captured bags, which are permeable for larval excretions/secretions, aid in healing severe, infected wounds, suspect for biofilm formation. Therefore we presumed maggot excretions/secretions would reduce biofilm formation. We studied biofilm formation of Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella oxytoca, Enterococcus faecalis, and Enterobacter cloacae on polyethylene, titanium, and stainless steel. We compared the quantities of biofilm formation between the bacterial species on the various biomaterials and the quantity of biofilm formation after various incubation times. Maggot excretions/secretions were added to existing biofilms to examine their effect. Comb-like models of the biomaterials, made to fit in a 96-well microtiter plate, were incubated with bacterial suspension. The formed biofilms were stained in crystal violet, which was eluted in ethanol. The optical density (at 595 nm) of the eluate was determined to quantify biofilm formation. Maggot excretions/secretions were pipetted in different concentrations to (nonstained) 7-day-old biofilms, incubated 24 hours, and finally measured. The strongest biofilms were formed by S. aureus and S. epidermidis on polyethylene and the weakest on titanium. The highest quantity of biofilm formation was reached within 7 days for both bacteria. The presence of excretions/secretions reduced biofilm formation on all biomaterials. A maximum of 92% of biofilm reduction was measured. Our observations suggest maggot excretions/secretions decrease biofilm formation and could provide a new treatment for biofilm formation on infected biomaterials.

  2. A novel serine protease secreted by medicinal maggots enhances plasminogen activator-induced fibrinolysis

    DEFF Research Database (Denmark)

    van der Plas, Mariena J A; Andersen, Anders S; Nazir, Sheresma

    2014-01-01

    Maggots of the blowfly Lucilia sericata are used for the treatment of chronic wounds. As haemostatic processes play an important role in wound healing, this study focused on the effects of maggot secretions on coagulation and fibrinolysis. The results showed that maggot secretions enhance plasmin...

  3. Clinical management of maggot wounds in Bengal Tigers (Panthera tigris tigris

    Directory of Open Access Journals (Sweden)

    Anup Kumar Talukder

    2017-03-01

    Full Text Available Objecitve: Maggot wound is common in domestic and pet animals but report on maggot wound treatment in wildlife species is scanty. The study reported here the surgical and conservative management of maggot wounds including anesthetic protocol and postoperative care in two Bengal tigers (Panthera tigris tigris. Materials and methods: One female and one male tiger were presented with maggot wounds for treatment at the Bangabandhu Sheikh Mujib Safari Park, Gazipur, Bangladesh. Tigers were anesthetized with combined injection of xylazine (dosed at 1.0 mg/kg bwt, IM and ketamine hydrochloride (dosed at 3.5 mg/kg bwt, IM. Superficial maggots were removed from wounds using sterile tissue forceps. Gauze soaked in oil of turpentine was allowed to remain in each wound pocket for 5 min for the removal of deep-seated maggots. Finally, wounds were dressed with tincture iodine to clean out the dead tissue debris and to induce inflammation for rapid healing. A single subcutaneous injection of ivermectin (dosed at 200 µg/kg bwt, IM was given in each tiger. In addition, long acting oxytetracycline (dosed at 10 mg/kg bwt, IM on 48 h interval for six days, chlorpheniramine maleate (dosed at 1 mg/kg bwt, IM once daily for three days, and ascorbic acid (dosed at 250 mg/tiger, IM once daily for seven days were administered in both tigers. Results: The tigers were recovered successfully without any complications in two weeks following treatment. Conclusion: Surgical management using oil of turpentine and tincture iodine along with parenteral administration of ivermectin, long acting oxytetracycline and chlorpheniramine maleate are effective for successful management of maggot wounds in Bengal tigers. [J Adv Vet Anim Res 2017; 4(1.000: 104-109

  4. Quorum-sensing-regulated virulence factors in Pseudomonas aeruginosa are toxic to Lucilia sericata maggots

    DEFF Research Database (Denmark)

    Andersen, A S; Joergensen, B; Bjarnsholt, T

    2010-01-01

    Maggot debridement therapy (MDT) is widely used for debridement of chronic infected wounds; however, for wounds harbouring specific bacteria limited effect or failure of the treatment has been described. Here we studied the survival of Lucilia sericata maggots encountering Pseudomonas aeruginosa...... PAO1 in a simple assay with emphasis on the quorum-sensing (QS)-regulated virulence. The maggots were challenged with GFP-tagged P. aeruginosa wild-type (WT) PAO1 and a GFP-tagged P. aeruginosa DeltalasR rhlR (DeltaRR) QS-deficient mutant in different concentrations. Maggots were killed...

  5. Yellow and the Novel Aposematic Signal, Red, Protect Delias Butterflies from Predators.

    Directory of Open Access Journals (Sweden)

    Jocelyn Liang Qi Wee

    Full Text Available Butterflies of the South Asian and Australian genus Delias possess striking colours on the ventral wings that are presumed to serve as warning signals to predators. However, this has not been shown empirically. Here we experimentally tested whether the colours of one member of this diverse genus, Delias hyparete, function as aposematic signals. We constructed artificial paper models with either a faithful colour representation of D. hyparete, or with all of its colours converted to grey scale. We also produced models where single colours were left intact, while others were converted to grey-scale or removed entirely. We placed all model types simultaneously in the field, attached to a live mealworm, and measured relative attack rates at three separate field sites. Faithful models of D. hyparete, suffered the least amount of attacks, followed by grey-scale models with unaltered red patches, and by grey-scale models with unaltered yellow patches. We conclude that red and yellow colours function as warning signals. By mapping dorsal and ventral colouration onto a phylogeny of Delias, we observed that yellow and red colours appear almost exclusively on the ventral wing surfaces, and that basal lineages have mostly yellow, white, and black wings, whereas derived lineages contain red colour in addition to the other colours. Red appears to be, thus, a novel adaptive trait in this lineage of butterflies.

  6. Yellow and the Novel Aposematic Signal, Red, Protect Delias Butterflies from Predators.

    Science.gov (United States)

    Wee, Jocelyn Liang Qi; Monteiro, Antónia

    2017-01-01

    Butterflies of the South Asian and Australian genus Delias possess striking colours on the ventral wings that are presumed to serve as warning signals to predators. However, this has not been shown empirically. Here we experimentally tested whether the colours of one member of this diverse genus, Delias hyparete, function as aposematic signals. We constructed artificial paper models with either a faithful colour representation of D. hyparete, or with all of its colours converted to grey scale. We also produced models where single colours were left intact, while others were converted to grey-scale or removed entirely. We placed all model types simultaneously in the field, attached to a live mealworm, and measured relative attack rates at three separate field sites. Faithful models of D. hyparete, suffered the least amount of attacks, followed by grey-scale models with unaltered red patches, and by grey-scale models with unaltered yellow patches. We conclude that red and yellow colours function as warning signals. By mapping dorsal and ventral colouration onto a phylogeny of Delias, we observed that yellow and red colours appear almost exclusively on the ventral wing surfaces, and that basal lineages have mostly yellow, white, and black wings, whereas derived lineages contain red colour in addition to the other colours. Red appears to be, thus, a novel adaptive trait in this lineage of butterflies.

  7. Economics of production of broiler chickens fed maggot meal as ...

    African Journals Online (AJOL)

    The economics of production of broiler chickens considered for 0-35 days, 35-56 days, and 0-56 days was compared for fishmeal (FM) and maggot meal (MM) diets. The maggot meal diet had a replacement of the fish meal at 75%> on protein basis. Replacing FM at 75% with MM resulted in reduced cost of feed as well as ...

  8. Maggot Debridement Therapy in Disaster Medicine.

    Science.gov (United States)

    Stadler, Frank; Shaban, Ramon Z; Tatham, Peter

    2016-02-01

    When disaster strikes, the number of patients requiring treatment can be overwhelming. In low-income countries, resources to assist the injured in a timely fashion may be limited. As a consequence, necrosis and wound infection in disaster patients is common and frequently leads to adverse health outcomes such as amputations, chronic wounds, and loss of life. In such compromised health care environments, low-tech and cheap wound care options are required that are in ready supply, easy to use, and have multiple therapeutic benefits. Maggot debridement therapy (MDT) is one such wound care option and may prove to be an invaluable tool in the treatment of wounds post-disaster. This report provides an overview of the wound burden experienced in various types of disaster, followed by a discussion of current treatment approaches, and the role MDT may play in the treatment of complex wounds in challenging health care conditions. Maggot debridement therapy removes necrotic and devitalized tissue, controls wound infection, and stimulates wound healing. These properties suggest that medicinal maggots could assist health care professionals in the debridement of disaster wounds, to control or prevent infection, and to prepare the wound bed for reconstructive surgery. Maggot debridement therapy-assisted wound care would be led by health care workers rather than physicians, which would allow the latter to focus on reconstructive and other surgical interventions. Moreover, MDT could provide a larger window for time-critical interventions, such as fasciotomies to treat compartment syndrome and amputations in case of life-threatening wound infection. There are social, medical, and logistic hurdles to overcome before MDT can become widely available in disaster medical aid. Thus, research is needed to further demonstrate the utility of MDT in Disaster Medicine. There is also a need for reliable MDT logistics and supply chain networks. Integration with other disaster management

  9. Maggot Therapy: The Science and Implication for CAM Part I—History and Bacterial Resistance

    Directory of Open Access Journals (Sweden)

    Yamni Nigam

    2006-01-01

    Full Text Available It is now a universally acknowledged fact that maggot therapy can be used successfully to treat chronic, long-standing, infected wounds, which have previously failed to respond to conventional treatment. Such wounds are typically characterized by the presence of necrotic tissue, underlying infection and poor healing. Maggot therapy employs the use of freshly emerged, sterile larvae of the common green-bottle fly, Phaenicia (Lucilia sericata, and is a form of artificially induced myiasis in a controlled clinical situation. In this review article, we will discuss the role of maggots and their preparation for clinical use. Maggot therapy has the following three core beneficial effects on a wound: debridement, disinfection and enhanced healing. In part I we explore our current understanding of the mechanisms underlying these effects.

  10. Mechanisms of Maggot-Induced Wound Healing: What Do We Know, and Where Do We Go from Here?

    Directory of Open Access Journals (Sweden)

    Ronald A. Sherman

    2014-01-01

    Full Text Available Medicinal maggots are believed to have three major mechanisms of action on wounds, brought about chemically and through physical contact: debridement (cleaning of debris, disinfection, and hastened wound healing. Until recently, most of the evidence for these claims was anecdotal; but the past 25 years have seen an increase in the use and study of maggot therapy. Controlled clinical studies are now available, along with laboratory investigations that examine the interaction of maggot and host on a cellular and molecular level. This review was undertaken to extract the salient data, make sense, where possible, of seemingly conflicting evidence, and reexamine our paradigm for maggot-induced wound healing. Clinical and laboratory data strongly support claims of effective and efficient debridement. Clinical evidence for hastened wound healing is meager, but laboratory studies and some small, replicated clinical studies strongly suggest that maggots do promote tissue growth and wound healing, though it is likely only during and shortly after the period when they are present on the wound. The best way to evaluate—and indeed realize—maggot-induced wound healing may be to use medicinal maggots as a “maintenance debridement” modality, applying them beyond the point of gross debridement.

  11. Maggot meal as a substitute for fish meal in laying chicken diet ...

    African Journals Online (AJOL)

    A 56-day experiment was conducted to determine thereplacement value of maggot meal for fish meal in diet of laying chicken. Fish meal was included at 30 g kg-1 of the control diet. Maggot meal incrementally replaced fish meal at 250, 500, 750, and 1000 g kg-1 on crude protein basis. The five diets were fed to a total of ...

  12. Maggot excretions/secretions are differentially effective against biofilms of Staphylococcus aureus and Pseudomonas aeruginosa

    DEFF Research Database (Denmark)

    van der Plas, Mariena J A; Jukema, Gerrolt N; Wai, Sin-Wen

    2008-01-01

    OBJECTIVES: Lucilia sericata maggots are successfully used for treating chronic wounds. As the healing process in these wounds is complicated by bacteria, particularly when residing in biofilms that protect them from antibiotics and the immune system, we assessed the effects of maggot excretions...

  13. STR analysis of human DNA from maggots fed on decomposing bodies: Assessment of the time period for successful analysis

    Directory of Open Access Journals (Sweden)

    Daniel Gachuiri Njau

    2016-09-01

    Full Text Available Frequently, forensic entomology is applied in the use of insect maggots for the identification of specimens or remains of humans. Maggot crop analysis could be valuable in criminal investigations when maggots are found at a crime scene and a corpse is absent. Human short tandem repeat (STR has previously been used to support the association of maggots to a specific corpse but not in the period at which the body has been decomposing. The aim of this research was to assess the time period for successful STR analyses of human DNA from third instar maggots (Protophormia terraenovae obtained from decomposing human corpses as well as to investigate the human DNA turnover and degradation in the maggot crop after they are removed from food and/or are fed on a beef (a new/different food source. Results showed that the amount of human DNA recovered from maggots decreased with time in all cases. For maggots fed on beef, the human DNA could only be recovered up to day two and up to day four for the starved maggots. STR analyses of human DNA from maggots’ crop content using 16 loci generated profiles that matched those of reference samples although some of the alleles were not amplifiable therefore generating partial profiles for the samples starved for 4 days and those fed on beef. This may be due to nuclease activity present in the gut of larvae that may have caused degradation of DNA and consequently reduction in DNA yield. It was possible to identify the decomposing body using STRs as markers.

  14. Onion seedlings versus onion sets in organic onion production

    OpenAIRE

    Kivijärvi, Pirjo; Hannukkala, Asko; Haapalainen, Minna; Iivonen, Sari

    2017-01-01

    The objective of this study is to find onion varieties that are suitable for seedling cultivation and high-yielding in our relatively cool and short growing season. In addition, the yield quantity, quality and long-term storage durability of onions produced from either seedlings or sets are compared. Rot incidence and composition of pathogen populations in diseased onions is monitored during the growing season and long-term storage. The field experiments and on-farm trials will be carried out...

  15. Rapid production of maggots as feed supplement and organic fertilizer by the two-stage composting of pig manure.

    Science.gov (United States)

    Zhu, Feng-Xiang; Wang, Wei-Ping; Hong, Chun-Lai; Feng, Ming-Guang; Xue, Zhi-Yong; Chen, Xiao-Yang; Yao, Yan-Lai; Yu, Man

    2012-07-01

    A two-stage composting experiment was performed to utilize pig manure for producing maggots as feed supplement and organic fertilizer. Seven-day composting of 1.8 ton fresh manure inoculated with 9 kg mixture of housefly neonates and wheat bran produced 193 kg aging maggots, followed by 12 week composting to maturity. Reaching the thermophilic phase and final maturity faster was characteristic of the maggot-treated compost compared with the same-size natural compost. Upon the transit of the maggot-treated compost to the second stage, the composting temperature maintained around 55 °C for 9 days and the moisture decreased to ~40%. Moreover, higher pH, faster detoxification and different activity patterns for some microbial enzymes were observed. There was a strong material loss (35% water-soluble carbon and 16% total nitrogen) caused by the maggot culture in the first stage. Our results highlight a higher economic value of pig manure achieved through the two-stage composting without bulking agents. Copyright © 2012 Elsevier Ltd. All rights reserved.

  16. Maggot debridement therapy promotes diabetic foot wound healing by up-regulating endothelial cell activity.

    Science.gov (United States)

    Sun, Xinjuan; Chen, Jin'an; Zhang, Jie; Wang, Wei; Sun, Jinshan; Wang, Aiping

    2016-03-01

    To determine the role of maggot debridement therapy (MDT) on diabetic foot wound healing, we compared growth related factors in wounds before and after treatment. Furthermore, we utilized human umbilical vein endothelial cells (HUVECs) to explore responses to maggot excretions/secretions on markers of angiogenesis and proliferation. The results showed that there was neo-granulation and angiogenesis in diabetic foot wounds after MDT. Moreover, significant elevation in CD34 and CD68 levels was also observed in treated wounds. In vitro, ES increased HUVEC proliferation, improved tube formation, and increased expression of vascular endothelial growth factor receptor 2 in a dose dependent manner. These results demonstrate that MDT and maggot ES can promote diabetic foot wound healing by up-regulating endothelial cell activity. Copyright © 2016. Published by Elsevier Inc.

  17. Examination of forensic entomology evidence using computed tomography scanning: case studies and refinement of techniques for estimating maggot mass volumes in bodies.

    Science.gov (United States)

    Johnson, Aidan; Archer, Melanie; Leigh-Shaw, Lyndie; Pais, Mike; O'Donnell, Chris; Wallman, James

    2012-09-01

    A new technique has recently been developed for estimating the volume of maggot masses on deceased persons using post-mortem CT scans. This allows volume to be measured non-invasively and factored into maggot mass temperature calculations for both casework and research. Examination of admission scans also allows exploration of entomological evidence in anatomical areas not usually exposed by autopsy (e.g. nasal cavities and facial sinuses), and before autopsy disrupts the maggot distribution on a body. This paper expands on work already completed by providing the x-ray attenuation coefficient by way of Hounsfield unit (HU) values for various maggot species, maggot masses and human tissue adjacent to masses. Specifically, this study looked at the HU values for four forensically important blowfly larvae: Lucilia cuprina, L. sericata, Calliphora stygia and C. vicina. The Calliphora species had significantly lower HU values than the Lucilia species. This might be explained by histological analysis, which revealed a non-significant trend, suggesting that Calliphora maggots have a higher fat content than the Lucilia maggots. It is apparent that the variation in the x-ray attenuation coefficient usually precludes its use as a tool for delineating the maggot mass from human tissue and that morphology is the dominant method for delineating a mass. This paper also includes three case studies, which reveal different applications for interpreting entomological evidence using post-mortem CT scans.

  18. Biological parameters of onion thrips, Thrips tabaci Lindeman on onion cultivars

    Directory of Open Access Journals (Sweden)

    Moraiet Maher Ahmed

    2017-06-01

    Full Text Available Biological parameters of the onion thrips, Thrips tabaci Lindeman were studied on the following onion (Allium cepa L. cultivars: Nasik Red Plus N-53, Onion Dr-301 (Krishna, Onion White, and Nasik Red, at 25±1°C and 65±5% RH. Significant (p < 0.05 differences were found in the life stages and fertility life tables on different cultivars except in the pupal stages. More information about the biological parameters of T. tabaci on onion cultivars can help in designing Integrated Pest Management programs for onion thrips.

  19. On mysterious occurrence of butterflies in the genus Delias at Jinmen Islands, with clarification on the nomenclature of D. pasithoe (Linnaeus, 1767) and D. acalis (Godart, 1819) in southern China.

    Science.gov (United States)

    Hsu, Yu-Feng; Huang, Hang-Chi; Lu, Chen-Chih

    2017-06-11

    In recent years, a considerable number of pierid butterflies of the genus Delias have been found from Jinmen Islands, where no available hostplants grow, rendering a proof of cross sea water migration of these butterflies. It is suggested here that these butterflies come from nearby continent of eastern China, visiting the islands for nectar acquisition in seasons when nectar is in short supply because they mostly show up in autumn months. Samples obtained during the survey contained two species, namely Delias pasithoe (Linnaeus, 1767) and Delias acalis (Godart, 1819). Subspecific names applied to both species in eastern China appear to involve nomenclatural problems, with erroneous usage in recent literature.

  20. Maggot debridement: an alternative method for debridement.

    Science.gov (United States)

    Gottrup, Finn; Jørgensen, Bo

    2011-01-01

    Debridement is an essential component to promote healing in a problem wound. Several techniques are available including maggot debridement therapy (MDT). To describe the efficacy of MDT for treating problem wound especially diabetic foot ulcers. The topic is elucidated from different points of view: the mode of action, when to use, use in a practice, clinical results, and discussing the problem of creating evidence for the clinical effect. Literature and own results demonstrate that MDT is a safe method with few side effects. Maggot debridement therapy is as good as or better than conventional often surgical debridement, is more selective than surgical debridement, decreases time to healing and stay of patients in the ward, and may decrease the risk of major amputations. However, the evidence of these effects of MDT on the highest level is presently lacking. A detailed description of how to use MDT in practice is provided including a visual demonstration in a video. In spite of lacking clinical evidence, MDT clinical experience strongly suggests that this technique is effective and safe. It can be used for most types of problem wounds, but our indication is primarily diabetic foot ulcers, because of its selectivity for debriding necrotic dead tissue. It may be a valuable alternative surgical/sharp debridement.

  1. Use of maggot therapy for treating a diabetic foot ulcer colonized by multidrug resistant bacteria in Brazil

    Directory of Open Access Journals (Sweden)

    Marilia A.R.Q. Pinheiro

    2015-01-01

    Full Text Available This study reports the efficacy of maggot therapy in the treatment of diabetic foot ulcer infected with multidrug resistant microorganisms. A 74 year old female patient with diabetes for over 30 years, was treated with maggot therapy using larvae of Chrysomya megacephala. The microbiological samples were collected to evaluate aetiology of the infection. The therapy done for 43 days resulted in a reduction of necrosis and the ulcer′s retraction of 0.7 cm [2] in area. Analysis of the bacteriological swabs revealed the presence of Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa. Further studies need to be done to confirm the role of maggot therapy in wound healing using a large sample and a proper study design.

  2. Maggot Debridement: An Alternative Method for Debridement

    OpenAIRE

    Gottrup, Finn; Jørgensen, Bo

    2011-01-01

    Debridement is an essential component to promote healing in a problem wound. Several techniques are available including maggot debridement therapy (MDT). Objective: To describe the efficacy of MDT for treating problem wound especially diabetic foot ulcers. Methods: The topic is elucidated from different points of view: the mode of action, when to use, use in a practice, clinical results, and discussing the problem of creating evidence for the clinical effect. Results: Literature and own resul...

  3. Maggot secretions skew monocyte-macrophage differentiation away from a pro-inflammatory to a pro-angiogenic type

    DEFF Research Database (Denmark)

    van der Plas, Mariena J A; van Dissel, Jaap T; Nibbering, Peter H

    2009-01-01

    BACKGROUND: Maggots of the blowfly Lucilia sericata are used for the treatment of chronic wounds. Earlier we reported maggot secretions to inhibit pro-inflammatory responses of human monocytes. The aim of this study was to investigate the effect of maggot secretions on the differentiation...... for 18 h. The expression of cell surface molecules and the levels of cytokines, chemokines and growth factors in supernatants were measured. Our results showed secretions to affect monocyte-macrophage differentiation leading to MØ-1 with a partial MØ-2-like morphology but lacking CD163, which...... is characteristic for MØ-2. In response to LPS or LTA, secretions-differentiated MØ-1 produced less pro-inflammatory cytokines (TNF-alpha, IL-12p40 and MIF) than control cells. Similar results were observed for MØ-2 when stimulated with low concentrations of LPS. Furthermore, secretions dose-dependently led to MØ-1...

  4. Hairy maggot of Chrysomya villeneuvi (Diptera: Calliphoridae), a fly species of forensic importance.

    Science.gov (United States)

    Sukontason, Kom; Sukontason, Kabkaew L; Piangjai, Somsak; Chaiwong, Tarinee; Boonchu, Noppawan; Kurahashi, Hiromu

    2003-11-01

    We reported on the hairy maggot of Chrysomya villeneuvi Patton, collected from a human corpse in Thailand. Although the general morphology of the third instar closely resembled the more common hairy maggot blow fly, Chrysomya rufifacies (Macquart), the spines along the tubercles of each body segment could be used as a feature to distinguish between these species, with those of C. villeneuri bearing sharp-ended spines that encircle the entire tubercle. Not only has the resemblance of a hairy appearance between C. villeneuvi and C. rufifacies larvae been documented but also their similarity in predacious feeding behavior. The differentiation between these two species is necessary for post-mortem interval estimation in the corpse.

  5. Maggot Instructor: Semi-Automated Analysis of Learning and Memory in Drosophila Larvae

    Directory of Open Access Journals (Sweden)

    Urte Tomasiunaite

    2018-06-01

    Full Text Available For several decades, Drosophila has been widely used as a suitable model organism to study the fundamental processes of associative olfactory learning and memory. More recently, this condition also became true for the Drosophila larva, which has become a focus for learning and memory studies based on a number of technical advances in the field of anatomical, molecular, and neuronal analyses. The ongoing efforts should be mentioned to reconstruct the complete connectome of the larval brain featuring a total of about 10,000 neurons and the development of neurogenic tools that allow individual manipulation of each neuron. By contrast, standardized behavioral assays that are commonly used to analyze learning and memory in Drosophila larvae exhibit no such technical development. Most commonly, a simple assay with Petri dishes and odor containers is used; in this method, the animals must be manually transferred in several steps. The behavioral approach is therefore labor-intensive and limits the capacity to conduct large-scale genetic screenings in small laboratories. To circumvent these limitations, we introduce a training device called the Maggot Instructor. This device allows automatic training up to 10 groups of larvae in parallel. To achieve such goal, we used fully automated, computer-controlled optogenetic activation of single olfactory neurons in combination with the application of electric shocks. We showed that Drosophila larvae trained with the Maggot Instructor establish an odor-specific memory, which is independent of handling and non-associative effects. The Maggot Instructor will allow to investigate the large collections of genetically modified larvae in a short period and with minimal human resources. Therefore, the Maggot Instructor should be able to help extensive behavioral experiments in Drosophila larvae to keep up with the current technical advancements. In the longer term, this condition will lead to a better understanding of

  6. Earthworm and maggots meals as potential fishmeal replacement

    OpenAIRE

    Yaqub, H.B.

    1997-01-01

    Three meals were formulated from the earthworm (Endrilus eugineae) and maggot (Musca domestica) and fish (Engraulis encrosicolus). These meals were evaluated as a potential replacement for fishmeal. This is because fishmeal could be very expensive at times. The three meals were used in feeding the catfish (Heterobranchus isopterus) fry for 30 days. The study was conducted in 1991 at the Institute of Renewable Natural Resources Farm, University of Science and Technology. Two replicate...

  7. Maggots in the Brain: Sequelae of Ignored Scalp Wound.

    Science.gov (United States)

    Aggarwal, Ashish; Maskara, Prasant

    2018-01-01

    A 26-year-old male had suffered a burn injury to his scalp in childhood and ignored it. He presented with a complaint of something crawling on his head. Inspection of his scalp revealed multiple maggots on the brain surface with erosion of overlying bone and scalp. He was successfully managed by surgical debridement and regular dressing. Copyright © 2017 Elsevier Inc. All rights reserved.

  8. Direct plantlet inoculation with soil or insect-associated fungi may control cabbage root fly maggots.

    Science.gov (United States)

    Razinger, Jaka; Lutz, Matthias; Schroers, Hans-Josef; Palmisano, Marilena; Wohler, Christian; Urek, Gregor; Grunder, Jürg

    2014-07-01

    A potential Delia radicum biological control strategy involving cauliflower plantlet inoculation with various fungi was investigated in a series of laboratory and glasshouse experiments. In addition to entomopathogenic fungi, fungi with a high rhizosphere competence and fungi with the ability to survive as saprotrophs in soil were tested. The following fungal species were evaluated in the experiments: Trichoderma atroviride, T. koningiopsis, T. gamsii, Beauveria bassiana, Metharhizium anisopliae, M. brunneum and Clonostachys solani. A commercial carbosulfan-based insecticide was used as a positive control. Additionally, two commercial products, one based on B. bassiana (Naturalis) and one on Bacillus thuringiensis (Delfin) were used as reference biocontrol agents. The aims were (i) to assess the pathogenicity of the selected fungal isolates to Delia radicum, (ii) to evaluate the fungal isolates' rhizosphere competence, with the emphasis on the persistence of the original inoculum on the growing roots, (iii) to assess possible endophytic plant tissue colonization, and (iv) to evaluate potential plant growth stimulating effects of the added inoculi. Significant pathogenicity of tested fungi against Delia radicum was confirmed in in vitro and glasshouse experiments. All tested fungi persisted on cauliflower rhizoplane. More importantly, the added fungi were found on thoroughly washed roots outside the original point of inoculation. This provided us with evidence that our tested fungi could be transferred via or grow with the elongating roots. In addition to colonizing the rhizoplane, some fungi were found inside the plant root or stem tissue, thus exhibiting endophytic characteristics. The importance of fungal ecology as a criterion in appropriate biological control agent selection is discussed. Copyright © 2014 Elsevier Inc. All rights reserved.

  9. Maggot therapy in treatment of a complex hand injury complicated by mycotic infection.

    Science.gov (United States)

    Bohac, M; Cambal, M; Zamborsky, R; Takac, P; Fedeles, J

    2015-01-01

    Complex injuries of the hand remain a therapeutic challenge for surgeons. We present the case of a male who suffered a devastating injury of the hand caused by a conveyor belt. The patient developed a progressive Absidia corymbifera infection of the affected soft tissues. Initial treatments with serial surgical debridement and topical and intravenous itraconazole were unsuccessful in eliminating the infection. We decided to use maggot debridement therapy in a new special design to debride all necrotic, devitalized tissue and preserve only healthy tissue and functioning structures. This maneuverer followed by negative pressure therapy allowed progressive healing. In such complex hand injuries, maggot debridement combined with negative pressure therapy could be considered to achieve effective and considerable results, although future functional morbidity may occur (Fig. 4, Ref. 18).

  10. Evaluation of Anti-adherent Activity of Excretions of Irradiated Lucilia sericata Maggot and Certain Essential Oils against Some Pathogenic Bacterial Strains

    International Nuclear Information System (INIS)

    Eltablawy, S.Y.; Amin, M.M.

    2011-01-01

    Essential Oils are widely used for their medicinal properties. They block adhesion and colonization of pathogenic microbes to epithelial cells which associated with bacterial resistance to antibiotics. So, this study investigates the effect of Lu cilia sacarato (flesh fly-an ectoparasitic) excretions of non-irradiated and irradiated maggot and some essential oils on biofilm formation by tube method, antimicrobial susceptibility by agar disc diffusion method as well as on their anti-adherent activity by spectrophotometric method. The results showed that excretions and secretions (E/S) of non-irradiated and irradiated maggots (at 20 Gy), as well as (clove and cinnamon oils) did not have antibacterial activity against the tested bacterial strains Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (St. aureus) and Staphylococcus epidermidis (St. epidermidis) except marjoram oil which has low antimicrobial activity against all the tested strains. The results also showed that the most potent oil was clove which decrease biofilm of P. aeruginosa by 83%, followed by marjoram (69%), then E/S of non-irradiated maggots (66%). Whiles, biofilm was less affected by cinnamon oil and E/S of irradiated maggots by 50 % and 36%, respectively. In addition, clove oil and E/S of non-irradiated maggots affect the pre-adhered biofilm of P. aeruginosa by 57 and 45 %, respectively. Conclusion: Clove oil flowed by marjoram had anti-adherent effect on P. aeruginosa. Greater inhibition of adhesion was observed by excretions of non-irradiated lucilia sericata.

  11. Impact of straw mulch on populations of onion thrips (Thysanoptera: Thripidae) in onion.

    Science.gov (United States)

    Larentzaki, E; Plate, J; Nault, B A; Shelton, A M

    2008-08-01

    Development of insecticide resistance in onion thrips, Thrips tabaci Lindeman (Thysanoptera: Thripidae), populations in onion (Allium spp.) fields and the incidence of the T. tabaci transmitted Iris yellow spot virus have stimulated interest in evaluating alternative management tactics. Effects of straw mulch applied in commercial onion fields in muck areas of western New York were assessed in 2006 and 2007 as a possible onion thrips management strategy. In trials in which no insecticides were applied for thrips control, straw mulch-treated plots supported significantly lower T. tabaci populations compared with control plots. In both years, the action thresholds of one or three larvae per leaf were reached in straw mulch treatments between 7 and 14 d later than in the control. Ground predatory fauna, as evaluated by pitfall trapping, was not increased by straw mulch in 2006; however, populations of the common predatory thrips Aeolothrips fasciatus (L.) (Thysanoptera: Aeolothripidae) were significantly lower in straw mulch plots in both years. Interference of straw mulch in the pupation and emergence of T. tabaci was investigated in the lab and their emergence was reduced by 54% compared with bare soil. In the field the overall yield of onions was not affected by the straw mulch treatment; however, the presence of jumbo grade onions (>77 mm) was increased in 2006, but not in 2007. These results indicate that populations of T. tabaci adults and larvae can be significantly reduced by the use of straw mulch without compromising overall onion yield. The use of this cultural practice in an onion integrated pest management program seems promising.

  12. PENGARUH TINGKAT SUBTITUSI TEPUNG IKAN DENGAN TEPUNG MAGGOT TERHADAP KOMPOSISI KIMIA PAKAN DAN TUBUH IKAN BANDENG (Chanos chanos Forsskal)

    OpenAIRE

    Putri, Dwi Septiani; Haryati; Zainuddin

    2011-01-01

    This study aims to determine the extent of substitution of fish meal with maggot meal as a protein source that can produce the chemical composition of feed and a good body for milkfish (Chanos chanos Forsskal). This study used a complete randomized design (CRD) with five treatments and three replications. Treatment to be tested is the substitution of fish meal with maggot meal as much as 0%, 25%, 50%, 75%, and 100%. Fish that used to have an average weight individual 0.84-0....

  13. Effect of replacing fish meal with maggot meal on growth nutrient ...

    African Journals Online (AJOL)

    One hundred and twenty (120) 4-week old finisher broilers of Ross breed were used to study the effect of feeding maggot meal replacing fish meal on growth performance, nutrient digestibility, carcass and organ characteristics. The birds were divided into 5 treatment groups identified as T1, T2, T3, T4 and T5 with 24 birds in ...

  14. Weed and Onion Response to multiple Applications of Goal Tender beginning at the 1-Leaf Stage of Onion

    Science.gov (United States)

    Broadleaf weed control in onion is difficult in part due to a lack of postemergence herbicide options at an early growth stage of onions. Onion tolerance to sequential applications of oxyfluorfen (Goal-Tender) alone and with bromoxynil (Buctril) beginning at the 1-lf stage of onions was evaluated n...

  15. Onion irradiation - a case study

    International Nuclear Information System (INIS)

    Huebner, G.

    1988-01-01

    The irradiation of onions (Allium cepa L.) serves to prevent sprouting associated with long-term storage or transport and storage of onions in climatic conditions which stimulate sprouting. JECFI the Joint Expert Committee for Food Irradiation of FAO/IAEA/WHO, recommended the application of an irradiation dose of up to 150 Gy for sprout inhibition with onions. (author)

  16. 'The Maggot Within': The state security apparatus in Ngũgĩ's Wizard ...

    African Journals Online (AJOL)

    'The Maggot Within': The state security apparatus in Ngũgĩ's Wizard of the Crow. ... the state security apparatus in Wizard of the Crow promote shady business deals and as well systematize corruption. ... AJOL African Journals Online. HOW TO ...

  17. Lucifensins, the Insect Defensins of Biomedical Importance: The Story behind Maggot Therapy

    Czech Academy of Sciences Publication Activity Database

    Čeřovský, Václav; Bém, R.

    2014-01-01

    Roč. 7, č. 3 (2014), s. 251-264 ISSN 1424-8247 R&D Projects: GA ČR GA203/08/0536 Institutional support: RVO:61388963 Keywords : antimicrobial peptide * insect defensin * lucifensin * maggot therapy * Lucilia sericata * Lucilia cuprina * peptide isolation * peptide identification Subject RIV: CC - Organic Chemistry http://www.mdpi.com/1424-8247/7/3/251

  18. Onions--a global benefit to health.

    Science.gov (United States)

    Griffiths, Gareth; Trueman, Laurence; Crowther, Timothy; Thomas, Brian; Smith, Brian

    2002-11-01

    Onion (Allium cepa L.) is botanically included in the Liliaceae and species are found across a wide range of latitudes and altitudes in Europe, Asia, N. America and Africa. World onion production has increased by at least 25% over the past 10 years with current production being around 44 million tonnes making it the second most important horticultural crop after tomatoes. Because of their storage characteristics and durability for shipping, onions have always been traded more widely than most vegetables. Onions are versatile and are often used as an ingredient in many dishes and are accepted by almost all traditions and cultures. Onion consumption is increasing significantly, particularly in the USA and this is partly because of heavy promotion that links flavour and health. Onions are rich in two chemical groups that have perceived benefits to human health. These are the flavonoids and the alk(en)yl cysteine sulphoxides (ACSOs). Two flavonoid subgroups are found in onion, the anthocyanins, which impart a red/purple colour to some varieties and flavanols such as quercetin and its derivatives responsible for the yellow and brown skins of many other varieties. The ACSOs are the flavour precursors, which, when cleaved by the enzyme alliinase, generate the characteristic odour and taste of onion. The downstream products are a complex mixture of compounds which include thiosulphinates, thiosulphonates, mono-, di- and tri-sulphides. Compounds from onion have been reported to have a range of health benefits which include anticarcinogenic properties, antiplatelet activity, antithrombotic activity, antiasthmatic and antibiotic effects. Here we review the agronomy of the onion crop, the biochemistry of the health compounds and report on recent clinical data obtained using extracts from this species. Where appropriate we have compared the data with that obtained from garlic (Allium sativum L.) for which more information is widely available. Copyright 2002 John Wiley & Sons

  19. Next Generation Sequencing Identifies Five Major Classes of Potentially Therapeutic Enzymes Secreted by Lucilia sericata Medical Maggots.

    Science.gov (United States)

    Franta, Zdeněk; Vogel, Heiko; Lehmann, Rüdiger; Rupp, Oliver; Goesmann, Alexander; Vilcinskas, Andreas

    2016-01-01

    Lucilia sericata larvae are used as an alternative treatment for recalcitrant and chronic wounds. Their excretions/secretions contain molecules that facilitate tissue debridement, disinfect, or accelerate wound healing and have therefore been recognized as a potential source of novel therapeutic compounds. Among the substances present in excretions/secretions various peptidase activities promoting the wound healing processes have been detected but the peptidases responsible for these activities remain mostly unidentified. To explore these enzymes we applied next generation sequencing to analyze the transcriptomes of different maggot tissues (salivary glands, gut, and crop) associated with the production of excretions/secretions and/or with digestion as well as the rest of the larval body. As a result we obtained more than 123.8 million paired-end reads, which were assembled de novo using Trinity and Oases assemblers, yielding 41,421 contigs with an N50 contig length of 2.22 kb and a total length of 67.79 Mb. BLASTp analysis against the MEROPS database identified 1729 contigs in 577 clusters encoding five peptidase classes (serine, cysteine, aspartic, threonine, and metallopeptidases), which were assigned to 26 clans, 48 families, and 185 peptidase species. The individual enzymes were differentially expressed among maggot tissues and included peptidase activities related to the therapeutic effects of maggot excretions/secretions.

  20. Genetic and Pathogenic Variability of Fusarium oxysporum f. sp. cepae Isolated from Onion and Welsh Onion in Japan.

    Science.gov (United States)

    Sasaki, Kazunori; Nakahara, Katsuya; Tanaka, Shuhei; Shigyo, Masayoshi; Ito, Shin-ichi

    2015-04-01

    Fusarium oxysporum f. sp. cepae causes Fusarium basal rot in onion (common onion) and Fusarium wilt in Welsh onion. Although these diseases have been detected in various areas in Japan, knowledge about the genetic and pathogenic variability of F. oxysporum f. sp. cepae is very limited. In this study, F. oxysporum f. sp. cepae was isolated from onion and Welsh onion grown in 12 locations in Japan, and a total of 55 F. oxysporum f. sp. cepae isolates (27 from onion and 28 from Welsh onion) were characterized based on their rDNA intergenic spacer (IGS) and translation elongation factor-1α (EF-1α) nucleotide sequences, vegetative compatibility groups (VCGs), and the presence of the SIX (secreted in xylem) homologs. Phylogenetic analysis of IGS sequences showed that these isolates were grouped into eight clades (A to H), and 20 onion isolates belonging to clade H were monophyletic and assigned to the same VCG. All the IGS-clade H isolates possessed homologs of SIX3, SIX5, and SIX7. The SIX3 homolog was located on a 4 Mb-sized chromosome in the IGS-clade H isolates. Pathogenicity tests using onion seedlings showed that all the isolates with high virulence were in the IGS-clade H. These results suggest that F. oxysporum f. sp. cepae isolates belonging to the IGS-clade H are genetically and pathogenically different from those belonging to the other IGS clades.

  1. Natural regulation of Delia radicum in organic cabbage production

    DEFF Research Database (Denmark)

    Meyling, Nicolai Vitt; Navntoft, Søren; Philipsen, Holger Frederik

    2013-01-01

    In a field experiment, we evaluated effects of three different organic white cabbage-cropping systems (O1, O2, O3) on the cabbage root fly, Delia radicum, and its egg predators and pupal parasitoids over 3 years. The three systems all complied with regulations for organic production, but varied...... in external nutrient input and N-recycling, and were compared to a conventionally farmed control. One organic system (O3) included an intercropped strip of green manure between crop rows. Oviposition by D. radicum was generally not reduced in organic cropping systems. However, higher pupae/egg ratios were...... observed in the conventional compared to all organic systems, indicating that immature survival from oviposition to pupation was reduced under all the three organic farming practices. In organic system O2 most small coleopteran predators were recorded, but predation on fly eggs was not significantly higher...

  2. Radiation treatment of onions

    International Nuclear Information System (INIS)

    Huebner, G.

    1988-06-01

    The comprehensive report comprises 15 papers closely related to the leading article. The irradiation of onions (Allium cepa L.) serves to prevent sprouting associated with long-term storage or transport and storage of onions in climatic conditions which stimulate sprouting. The papers contain results of physiological investigations on the optimum conditions for onion irradiation, and technical and economic parameters of the process under national economy and business management aspects. For two different types of irradiation plants, a bulk-type plant and a multi-purpose large-scale irradiation plant, technical specifications and economic results in relation to product throughput, loading and price of 60 Co are presented. (author)

  3. Irradiation of onions with the commercial potato irradiator

    International Nuclear Information System (INIS)

    Aoki, Shohei; Kawashima, Koji; Hayasi, Toru

    1983-01-01

    Three varieties of onion harvested in Hokkaido were irradiated with the Shohoro Potato Irradiator on 29th September, 1981. One ton of each of the varieties, Kitamiki (KI), Ohohtsuku (OH) and Furanui (FU), was used in this investigation. Onions had longer dormancy period in the order of FU>OH>KI. Higher sprouting percentage was obtained in the unirradiated onions, while they were stored at a higher temperature or stored for a longer period. Generally, unirradiated onions sprouted before they were deteriorated. Thus the number of deteriorated bulbs in the unirradiated onions was superficially less than that in the irradiated ones. When the onions which were taken from warehouses on 26th March, 1982 were stored at room temperature, the percentage of wholesome bulbs was higher in KI and OH than FU. Small buds in some of the irradiated onions turned dark after a long storage time. Quantitative estimation of this phenomena is left to be resolved. There was little relationship between the weight loss and the number of wholesome onions. (author)

  4. Irradiation of onions on a large scale

    International Nuclear Information System (INIS)

    Kawashima, Koji; Hayashi, Toru; Uozumi, J.; Sugimoto, Toshio; Aoki, Shohei

    1984-01-01

    A large number of onions of var. Kitamiki and Ohotsuku were irradiated in September followed by storage at 0 deg C or 5 deg C. The onions were shifted from cold-storage facilities to room temperature in mid-March or in mid-April in the following year. Their sprouting, rooting, spoilage characteristics and sugar content were observed during storage at room temperature. Most of the unirradiated onions sprouted either outside or inside bulbs during storage at room temperature, and almost all of the irradiated ones showed small buds with browning inside the bulb in mid-April irrespective of the storage temperature. Rooting and/or expansion of bottom were observed in the unirradiated samples. Although the irradiated materials did not have root, they showed expansion of bottom to some extent. Both the irradiated and unirradiated onions spoiled slightly unless they sprouted, and sprouted onions got easily spoiled. There was no difference in the glucose content between the unirradiated and irradiated onions, but the irradiated ones yielded higher sucrose content when stored at room temperature. Irradiation treatment did not have an obvious effect on the quality of freeze-dried onion slices. (author)

  5. Idaho–Eastern Oregon Onion Industry Analysis

    OpenAIRE

    Bolotova, Yuliya; Jemmett, Brian

    2010-01-01

    The Idaho–Eastern Oregon onion industry operates in a market environment characterized by a high level of onion price and supply volatility. Years of relatively high onion prices are often followed by years of very low prices which do not allow onion growers to recover their onion production costs. This feature of the industry adversely affects the profi tability of onion growers and the economic performance of their industry. This study conducts an analysis of alternative market scenarios ...

  6. Sprout inhibition of onions by irradiation

    International Nuclear Information System (INIS)

    Siddiqui, A.K.; Hossain, M.A.; Choudhury, M.S.U.; Matin, M.A.; Amin, M.R.; Hossain, M.M.

    1979-01-01

    Onions of a set grown local variety were gamma irradiated in the dose range of 2-8 Krad. The irradiated onions were then stored in 10 lb. bags and in single layer at an average ambient temperature of 83 0 F and 79% relative humidity. Radiation doses of 4 Krad and above were completely inhibitory to sprouting of onions. Weight loss and rot incidence were markedly reduced in irradiated samples up to 10 months in both jute bag and single layer storage. An analysis of the cost benefit revealed that commercial irradiation of onions is economically feasible in Bangladesh if post-irradiation of storage extends for a period of over 6 months. (author)

  7. Anti Leishmania activity of Lucilia sericata and Calliphora vicina maggots in laboratory models.

    Science.gov (United States)

    Sanei-Dehkordi, Alireza; Khamesipour, Ali; Akbarzadeh, Kamran; Akhavan, Amir Ahmad; Mir Amin Mohammadi, Akram; Mohammadi, Younes; Rassi, Yavar; Oshaghi, Mohammad Ali; Alebrahim, Zahra; Eskandari, Seyed Ebrahim; Rafinejad, Javad

    2016-11-01

    Use of sterile fly larvae (maggots) of blow flies for the treatment of many different types of skin and soft tissue wounds is called Maggot debridement therapy (MDT). The larvae of blow flies secrete a broad spectrum of compounds with diverse mechanisms of action in the gut and salivary glands called excretion/secretion (ES) products which showed to have antimicrobial activities against Gram negative and positive bacteria. Cutaneous leishmaniasis (CL) which is the common form of leishmaniasis is difficult to treat. In this study, the effect of ES from 2 nd and 3 rd stages of L. sericata and C. vicina larvae on in vitro Leishmania major amastigote growth in macrophage was evaluated. The effect of ES on Leishmania growth was estimated by assessing the rate of macrophage infection and the number of amastigotes per infected macrophages. In addition, the anti Leishmania activities of larval and ES of L. sericata and C. vicina on the skin lesion induced by L. major infection was evaluated in susceptible BALB/c mice. The results showed that ES of both flies reduced the number of infected macrophages; 2.6 and 1.5-fold using L. sericata ES and C. vicina ES, respectively, and inhibited amastigotes growth in macrophages; 2.03 and 1.36-fold by L. sericata ES and C. vicina ES, respectively as compared to the control group. The results showed that L. sericata ES was significantly more effective than C. vicina ES to inhibit in vitro L. major amastigotes growth, The size of lesion was significantly smaller in BALB/c mice treated with L. sericata ES than treated with C. vicina ES. The results of in vivo experiments suggested that pre-treatment with ES derived from L. sericata may have some protective effects on the development of L. major lesion. Therefore, it seems that maggot ES might be considered as a possible candidate for the treatment of cutaneous leishmaniasis. Copyright © 2016 Elsevier Inc. All rights reserved.

  8. Activitie of polyphenoloxidase in yellow and purple onion

    Directory of Open Access Journals (Sweden)

    Joeliton Alves Calado

    2018-01-01

    Full Text Available The high perishability of the onion may limit the shelf life of the bulbs, mainly by the biochemical transformations. The objective of this work is to determine and compare polyphenoloxidase activity (PPO in yellow and purple onions and their functional body. The raw material was purchased in the local market , where they were transported to the Food Analysis Laboratory of the, Center for Agro-Food Science and Technology, Federal University of Campina Grande, Campus Pombal, being selected, cleaned, sorted, peeled processed and carried out chemical and physico- chemical analysis and determination of the enzymatic activity of polyphenol oxidase (PPO. The statistical design was completely randomized with two treatments (purple and yellow onion with six repetitions. The purple onion presented levels of soluble solids, flavonoids and anthocyanins, more significant than the white onion. Both onions showed enzymatic activity, without significant differences.

  9. Detection of radiation processing in onions

    International Nuclear Information System (INIS)

    Duchacek, V.

    1985-01-01

    Two breeds of onions were used for irradiation. Both breeds were divided into two parts - the first was irradiated with a dose of 80 Gy and the second served as a control. The two parts were stored under the same conditions. Conductometry, liquid chromatography and spectrophotometry were used for detecting the radiation processing of the onions. Only from the spectrophotometric determination of 2-desoxysaccharides it was possible to safely distinguish irradiated onions from non-irradiated controls throughout storage time. (E.S.)

  10. Simultaneous Detection of Mixed Infection of Onion yellow dwarf virus and an Allexivirus in RT-PCR for Ensuring Virus Free Onion Bulbs.

    Science.gov (United States)

    Kumar, Sandeep; Baranwal, V K; Joshi, Subodh; Arya, Meenakshi; Majumder, S

    2010-06-01

    Reduced seed production in onion is associated with Onion yellow dwarf virus (OYDV), a filamentous Potyvirus. Onion is also infected with other filamentous virus particles suspected to be Allexivirus. RT-PCR was used to detect mixed infection of both the viruses in leaves and bulbs. A duplex RT-PCR was developed, which simultaneously detected the presence of these two viruses in winter (Rabi) onion bulb. In summer (Kharif) onion bulbs only Allexivirus was detected. The absence of OYDV in summer crop is discussed. The sequencing of RT-PCR amplified products confirmed the identity of OYDV and Allexivirus, the latter showing closer identity to Garlic virus C (GVC)/Garlic mite-borne mosaic virus. This makes the first detection of an Allexivirus in onion crop in India. The duplex RT-PCR to detect these viruses (OYDV and Allexivirus) would be an improvement for indexing of viruses in onion bulbs for seed production.

  11. Combinations of maggot excretions/secretions and antibiotics are effective against Staphylococcus aureus biofilms and the bacteria derived therefrom

    DEFF Research Database (Denmark)

    van der Plas, Mariena J A; Dambrot, Cheryl; Dogterom-Ballering, Heleen C M

    2010-01-01

    OBJECTIVES: Maggots of the blowfly Lucilia sericata are used for the treatment of chronic wounds. Previously we reported that maggot excretions/secretions (ES) break down Staphylococcus aureus biofilms but do not kill the bacteria. As many antibiotics are not effective against biofilms we assessed...... the effect of combinations of ES and antibiotics on S. aureus biofilms and on the survival of the bacteria released from the biofilms. METHODS: Effects of ES, antibiotics (vancomycin, daptomycin or clindamycin) and combinations thereof on S. aureus ATCC 29 213 biofilms and bacterial viability were determined...... using microtitre plates and in vitro killing assays. RESULTS: Vancomycin and daptomycin dose-dependently enhanced biofilm formation, whereas clindamycin reduced S. aureus biofilm size. Adding ES to antibiotic incubations caused a complete biofilm breakdown. After a lag time the bacteria derived from...

  12. Profitability and Efficiency of Red Onion Farming

    Directory of Open Access Journals (Sweden)

    Imron Rosyadi

    2014-12-01

    Full Text Available The purpose of this research is to determine and analyze the profitability and performance of onion farming marketing margins; analyze and know the parts of prices received by farmers and analyze the efficiency of onion farming in the district of Brebes. Samples taken in this study is 30 onion farmers in the district of Brebes, who settled in six villages, each village was taken 5 farmers as the research sample. These results indicate that the location of onion farming research does not provide benefits significantly to the household economy of farmers. Higher selling prices at the retail level and supermarkets do not have a significant impact on the level of profits of farming in the study area. Farming is done by farmers in the study area is inefficient. Onion marketing chain in the study area is relatively long, which consists of 4 lines of marketing.

  13. Pilot-scale studies on the irradiation of onions in Hungary

    International Nuclear Information System (INIS)

    Kalman, B.; Kiss, I.; Farkas, J.

    1978-01-01

    Various lots of onions from 120 to 10,000kg were irradiated with a 5krad dose 2 to 7 weeks after harvest in 1973 to 1976 and stored until March-April the following year. During the final period of storage the percentage amounts of sprouted and/or decayed onions were measured, the yield of peeled onions acceptable for dehydration processing was established and the quality of the dehydrated onion flakes was investigated. On average an 8-10% reduction in the total storage losses could be achieved by irradiation. The increased storability of irradiated onions could allow an extension of about 3 weeks in the dehydration season for the processing industry. When irradiated onions were processed in February or March the yield of onions peeled for dehydration was about 10% higher on average than that of untreated onions. The quality of the dried product made from irradiated onions was better than that of the control because 30-70% less bud parts occurred than in the untreated product and they were less discoloured. Irradiated onions showed excellent storage qualities in household tests performed in April and May 1976. The marketing trials in 1976 and 1977 in two different areas demonstrated that the Hungarian consumers had no objection to purchasing irradiated onions. (author)

  14. A less energy intensive process for dehydrating onion

    OpenAIRE

    Grewal, Manpreet Kaur; Jha, S. N.; Patil, R. T.; Dhatt, A. S.; Kaur, Amandeep; Jaiswal, P.

    2013-01-01

    Onion powder has an extensive demand and wide application worldwide as flavour additive in convenience foods and medicinal products. Conventionally onion powder is prepared by hot air drying of onion slices followed by grinding. Convective air drying when used alone demands longer drying time and thus has a high expense of energy. As bulk of onion is water (82–87 %), removal of moisture prior to drying can reduce moisture loading on dryer and hence the energy consumption. Keeping this in view...

  15. 7 CFR 457.135 - Onion crop insurance provisions.

    Science.gov (United States)

    2010-01-01

    ... Basic Provisions with (1) controlling (2) etc. 1. Definitions Damaged onion production. Storage type... method and production practice. Harvest. Removal of the onions from the field after topping and lifting... production. Onions of recoverable size and condition, with excess dirt and foliage material removed and that...

  16. 76 FR 43606 - Common Crop Insurance Regulations; Onion Crop Insurance Provisions

    Science.gov (United States)

    2011-07-21

    ... higher first stage production guarantee for most onion producing areas. Also, a contracted onion crop... for the damaged onion acreage, then any later appraised unharvested production or harvested production...'', ``Onion production'', ``Production guarantee (per acre)'', ``Storage onions'', ``Topping'', ``Transplanted...

  17. Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina.

    Science.gov (United States)

    Rinland, María Emilia; Gómez, Marisa Anahí

    2015-03-01

    Onion production in Argentina generates a significant amount of waste. Finding an effective method to recycle it is a matter of environmental concern. Among organic waste reuse techniques, anaerobic digestion could be a valuable alternative to current practices. Substrate inoculation with appropriate bacterial strains enhances the rate-limiting step (hydrolysis) of anaerobic digestion of biomass wastes. Selection of indigenous bacteria with the ability to degrade onion waste could be a good approach to find a suitable bioaugmentation or pretreatment agent. We isolated bacterial strains from onion waste in different degradation stages and from different localities. In order to characterize and select the best candidates, we analyzed the growth patterns of the isolates in a medium prepared with onion juice as the main source of nutrients and we evaluated carbon source utilization. Nine strains were selected to test their ability to grow using onion tissue and the five most remarkable ones were identified by 16S rRNA gene sequencing. Strains belonged to the genera Pseudoxanthomonas, Bacillus, Micrococcus and Pseudomonas. Two strains, Bacillus subtilis subsp. subtillis MB2-62 and Pseudomonas poae VE-74 have characteristics that make them promising candidates for bioaugmentation or pretreatment purposes.

  18. Field-based Evaluation of a Novel SPME-GC-MS Method for Investigation of Below-ground Interaction between Brassica Roots and Larvae of Cabbage Root Fly, Delia radicum L.

    Science.gov (United States)

    Deasy, William; Shepherd, Tom; Alexander, Colin J; Birch, A Nicholas E; Evans, K Andrew

    2016-11-01

    Collection of volatiles from plant roots poses technical challenges due to difficulties accessing the soil environment without damaging the roots. To validate a new non-invasive method for passive sampling of root volatiles in situ, from plants grown under field conditions, using solid phase micro-extraction (SPME). SPME fibres were inserted into perforated polytetrafluoroethene (PTFE) tubes positioned in the soil next to broccoli plants for collection of root volatiles pre- and post-infestation with Delia radicum larvae. After sample analysis by gas chromatography-mass spectrometry (GC-MS), principal component analysis (PCA) was applied to determine differences in the profiles of volatiles between samples. GC-MS analysis revealed that this method can detect temporal changes in root volatiles emitted before and after Delia radicum damage. PCA showed that samples collected pre- and post-infestation were compositionally different due to the presence of root volatiles induced by D. radicum feeding. Sulphur containing compounds, in particular, accounted for the differences observed. Root volatiles emission patterns post-infestation are thought to follow the feeding and developmental progress of larvae. This study shows that volatiles released by broccoli roots can be collected in situ using SPME fibres within perforated PTFE tubes under field conditions. Plants damaged by Delia radicum larvae could be distinguished from plants sampled pre-infestation and soil controls on the basis of larval feeding-induced sulphur-containing volatiles. These results show that this new method is a powerful tool for non-invasive sampling of root volatiles below-ground. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  19. Storage tests with irradiated and non-irradiated onions

    International Nuclear Information System (INIS)

    Gruenewald, T.; Rumpf, G.; Troemel, I.; Bundesforschungsanstalt fuer Ernaehrung, Karlsruhe

    1978-07-01

    The results of several test series on the storage of irradiated and non-irradiated German grown onion are reported. Investigated was the influence of the irradiation conditions such as time and dose and of the storage conditions on sprouting, spoilage, browning of the vegetation centres, composition of the onions, strength and sensorial properties of seven different onion varieties. If the onions were irradiated during the dormancy period following harvest, a dose of 50 Gy (krad) was sufficient to prevent sprouting. Regarding the irradiated onions, it was not possible by variation of the storage conditions within the limits set by practical requirements to extend the dormancy period or to prevent browning of the vegetation centres, however. (orig.) 891 MG 892 RSW [de

  20. Comparative profitability of onions harvested as green and dry (mature in Botswana

    Directory of Open Access Journals (Sweden)

    S.P. Baliyan

    2013-06-01

    Full Text Available This study was an attempt to calculate and compare the profitability of onions harvested as green and dry (mature in Botswana. Half of the planted onions were harvested and sold as green and half were harvested and sold as dry onions. The cost of production of green onions was 32.78% higher than the cost of production of dry onions. The irrigation and marketing expenses contributed the highest difference in the cost of production of green and dry onions. The major cost item contributing to the cost of green onions production was marketing cost (32.86% followed by irrigation cost (23.77% and harvesting cost (18.53% whereas the highest cost of production for dry onions was contributed by irrigation (38.58% followed by marketing (19.45% and planting (11.96%. The marketing cost for green onions was almost double (35.6% as compare to the dry onions (18.2%. The total return from green onions was 50.90% higher than the returns from dry onions. Gross margin of onions harvested as green was 63% higher than the gross margin from dry onions, which indicated that the production of green onions is more profitable as compare to production of dry onions. The farmers preferred onion harvested as green because it generates regular and higher returns than onions harvested as mature. Government should support farmers through some policies such as Minimum Support Price (MSP for dry onions, distribution of Mini Ferti–Seed Kit (Seeds of improved varieties and fertilizer package, construction of storages and formation of cooperatives.

  1. 7 CFR 956.5 - Walla Walla Sweet Onions.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Walla Walla Sweet Onions. 956.5 Section 956.5... Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE SWEET ONIONS GROWN IN THE WALLA WALLA VALLEY OF SOUTHEAST WASHINGTON AND NORTHEAST OREGON Definitions § 956.5 Walla Walla Sweet Onions...

  2. History of the sterile insect technique

    International Nuclear Information System (INIS)

    Klassen, W.; Curtis, C.F.

    2005-01-01

    During the 1930s and 1940s the idea of releasing insects of pest species to introduce sterility (sterile insect technique or SIT) into wild populations, and thus control them, was independently conceived in three extremely diverse intellectual environments. The key researchers were A. S. Serebrovskii at Moscow State University, F. L. Vanderplank at a tsetse field research station in rural Tanganyika (now Tanzania), and E. F. Knipling of the United States Department of Agriculture. Serebrovskii's work on chromosomal translocations for pest population suppression could not succeed in the catastrophic conditions in the USSR during World War II, after which he died. Vanderplank used hybrid sterility to suppress a tsetse population in a large field experiment, but lacked the resources to develop this method further. Knipling and his team exploited H. J. Muller's discovery that ionizing radiation can induce dominant lethal mutations, and after World War II this approach was applied on an area-wide basis to eradicate the New World screwworm Cochliomyia hominivorax (Coquerel) in the USA, Mexico, and Central America. Since then very effective programmes integrating the SIT have been mounted against tropical fruit flies, some species of tsetse flies Glossina spp., the pink bollworm Pectinophora gossypiella (Saunders), and the codling moth Cydia pomonella (L.). In non-isolated onion fields in the Netherlands, the onion maggot Delia antiqua (Meigen) has since 1981 been suppressed by the SIT. In the 1970s there was much research conducted on mosquito SIT, which then went into 'eclipse', but now appears to be reviving. Development of the SIT for use against the boll weevil Anthonomus grandis grandis Boheman and the gypsy moth Lymantria dispar (L.) has ended, but it is in progress for two sweetpotato weevil species, Cylas formicarius (F.) and Euscepes postfasciatus (Fairmaire), the false codling moth Cryptophlebia leucotreta (Meyrick), the carob moth Ectomyelois ceratoniae

  3. Using Sphinx to Improve Onion Routing Circuit Construction

    Science.gov (United States)

    Kate, Aniket; Goldberg, Ian

    This paper presents compact message formats for onion routing circuit construction using the Sphinx methodology developed for mixes. We significantly compress the circuit construction messages for three onion routing protocols that have emerged as enhancements to the Tor anonymizing network; namely, Tor with predistributed Diffie-Hellman values, pairing-based onion routing, and certificateless onion routing. Our new circuit constructions are also secure in the universal composability framework, a property that was missing from the original constructions. Further, we compare the performance of our schemes with their older counterparts as well as with each other.

  4. 77 FR 13961 - Common Crop Insurance Regulations; Onion Crop Insurance Provisions

    Science.gov (United States)

    2012-03-08

    ... regarding the definition of ``onion production.'' The commenters stated FCIC proposed removing the phrase... it will mean that onions that are too small to recover could be considered ``onion production.'' The definition of ``damaged onion production'' includes onions that do not meet certain grade standards. The...

  5. The Prevalence of Sheep Traumatic Myiasis in Three Counties from the West Side of Romania and Bacteria Isolated from the Insects Maggots

    Directory of Open Access Journals (Sweden)

    Daniela Marina Mot

    2013-10-01

    Full Text Available Myiasis represents an infestation of animals and humans caused by the maggots of certain fly species of Diptera order, Insecta class, which feed on the hosts' living or dead tissues or body fluids. In sheep, myiasis is a major animal welfare issue developing serious pain, suffering and in untreated cases may result in tissue injuries, reproduction and productivity losses and even death. There are two most important fly species which cause traumatic cutaneous myiasis of sheep in Europe: Wohlfahrtia magnifica (Sarcophagidae implicated in etiology of wound myiasis in southern and eastern Europe and Lucilia sericata (Calliphoridae, implicated in etiology of sheep strike, mainly in the middle latitudes of Europe continent. A few farmers from Timiş, Arad and Caraş-Severin counties were been asked to response to a questionnaire on the prevalence of traumatic myiasis which evolved in their sheep flock in April-September period of year 2012. From a total number of 2206 sheep taken into study were been discovered 1658 healthy sheep (75.16% and 548 sheep with myiasis (24.84%. From identified lesions with myiasis were been collected insects maggots from all three stages of development and were been prepared in Microbiology laboratory in the view to obtain data on the culturable bacteria isolated under aerobic conditions. Bacteria detected from maggots samples were: Staphylococcus aureus, Bacillus subtilis, Proteus vulgaris, Micrococcus luteus and Escherichia coli. The myasis insects maggots in sheep infestation can acquire many bacteria from their host or from their surroundings, all these can, together another bacteria, complicate the lesions and without treatment may lead to animals death.

  6. Exogenous ethylene inhibits sprout growth in onion bulbs.

    Science.gov (United States)

    Bufler, Gebhard

    2009-01-01

    Exogenous ethylene has recently gained commercial interest as a sprouting inhibitor of onion bulbs. The role of ethylene in dormancy and sprouting of onions, however, is not known. A cultivar (Allium cepa 'Copra') with a true period of dormancy was used. Dormant and sprouting states of onion bulbs were treated with supposedly saturating doses of ethylene or with the ethylene-action inhibitor 1-methylcyclopropene (1-MCP). Initial sprouting was determined during storage at 18 degrees C by monitoring leaf blade elongation in a specific size class of leaf sheaths. Changes in ATP content and sucrose synthase activity in the sprout leaves, indicators of the sprouting state, were determined. CO(2) and ethylene production of onion bulbs during storage were recorded. Exogenous ethylene suppressed sprout growth of both dormant and already sprouting onion bulbs by inhibiting leaf blade elongation. In contrast to this growth-inhibiting effect, ethylene stimulated CO(2) production by the bulbs about 2-fold. The duration of dormancy was not significantly affected by exogenous ethylene. However, treatment of dormant bulbs with 1-MCP caused premature sprouting. Exogenous ethylene proved to be a powerful inhibitor of sprout growth in onion bulbs. The dormancy breaking effect of 1-MCP indicates a regulatory role of endogenous ethylene in onion bulb dormancy.

  7. Steam-cooking rapidly destroys and reverses onion-induced antiplatelet activity

    Directory of Open Access Journals (Sweden)

    Hansen Emilie A

    2012-09-01

    Full Text Available Abstract Background Foods in the diet that can aid in the prevention of diseases are of major interest. Onions are key ingredients in many cuisines around the world and moreover, onion demand has trended higher over the past three decades. An important pharmacological aspect of onion is the ability to inhibit platelet aggregation. Raw onions inhibit platelet aggregation; however, when onions are boiled or heated, antiplatelet activity may be abolished. Methods Onion quarters were steamed for 0, 1, 3, 6, 10, and 15 min. The in vitro antiplatelet activity of a yellow hybrid storage onion was examined at these times on the blood of 12 human subjects using in vitro whole blood aggregometry. Results Contrary to findings reported for boiling, antiplatelet activity was destroyed between 3 and 6 min of steaming, and at 10 min of steaming, cooked onions stimulated platelet activity. Extracts from cooked onion had the potential to reverse the inhibitory effect on blood platelets by 25%. Responses were consistent across all donors. Total polyphenolic concentration and soluble solids were not affected by steaming time. Conclusions The potential value of cooked onion preparations may result in destruction or reversal of antiplatelet activity, without affecting the polyphenolic concentration.

  8. HPLC-DAD determination of imidacloprid in onion

    OpenAIRE

    Mandić Aljoša; Lazić Sanja; Inđić Dušanka

    2003-01-01

    Imidacloprid is an insecticide most commonly used on vegetables, potato sugar beet, fruit, cereal, maize and rice. Imidacloprid residue has been determined in spiked onion and in onion samples. Sample preparation consisted of dichlormethane extraction of imidacloprid from onion, followed by purification of the obtained extract on a LC-Florisil disposable cartridge. The HPLC-DAD method bas been developed on reversed-phase for separation of imidacloprid with a mixture of 0.01 M phosphate buffer...

  9. 78 FR 45898 - Vidalia Onions Grown in Georgia; Continuance Referendum

    Science.gov (United States)

    2013-07-30

    ... Vidalia onions produced in the production area. DATES: The referendum will be conducted from September 9... through September 27, 2013, among Vidalia onion producers in the production area. Only Vidalia onion producers that were engaged in the production of Vidalia onions in Georgia during the period of January 1...

  10. Effect of arbuscular mycorrhizal fungi on onion growth and onion stunting caused by Rhizoctonia solani, 2013.

    Science.gov (United States)

    A preliminary study was conducted in a greenhouse (15 ± 1oC, with supplemental lights for 12 h/day) to determine the role of AMF on onion growth and for reducing the severity of onion stunting, using a commercial AMF inoculant, BioTerra Plus, that contains 104 propagules/g (ppg) of Glomus intraradic...

  11. The role of onion-associated fungi in bulb mite infestation and damage to onion seedlings.

    Science.gov (United States)

    Ofek, Tal; Gal, Shira; Inbar, Moshe; Lebiush-Mordechai, Sara; Tsror, Leah; Palevsky, Eric

    2014-04-01

    In Israel Rhizoglyphus robini is considered to be a pest in its own right, even though the mite is usually found in association with fungal pathogens. Plant protection recommendations are therefore to treat germinating onions seedlings, clearly a crucial phase in crop production, when mites are discovered. The aim of this study was to determine the role of fungi in bulb mite infestation and damage to germinating onion seedlings. Accordingly we (1) evaluated the effect of the mite on onion seedling germination and survival without fungi, (2) compared the attraction of the mite to species and isolates of various fungi, (3) assessed the effect of a relatively non-pathogenic isolate of Fusarium oxysporum on mite fecundity, and (4) determined the effects of the mite and of F. oxysporum separately and together, on onion seedling germination and sprout development. A significant reduction of seedling survival was recorded only in the 1,000 mites/pot treatment, after 4 weeks. Mites were attracted to 6 out of 7 collected fungi isolates. Mite fecundity on onion sprouts infested with F. oxysporum was higher than on non-infested sprouts. Survival of seedlings was affected by mites, fungi, and their combination. Sprouts on Petri dishes after 5 days were significantly longer in the control and mite treatments than both fungi treatments. During the 5-day experiment more mites were always found on the fungi-infected sprouts than on the non-infected sprouts. Future research using suppressive soils to suppress soil pathogens and subsequent mite damage is proposed.

  12. Insecticides are not always the answer for combatting pests in onion fields

    OpenAIRE

    Hutchinson, Harlie

    2016-01-01

    Onion thrips are the insect vector of a severe virus in onion, Iris yellow spot virus. The thrips and virus are primary threats to the economic stability of onion production worldwide. Overuse of insecticides to suppress onion thrips has resulted in the development of resistance, reduced performance of insecticides, and reduced onion yields. There is a compelling desire to find alternatives to better manage these pests. In this study, we assessed onion thrips populations on onions with low...

  13. Production and characterization of tearless and non-pungent onion.

    Science.gov (United States)

    Kato, Masahiro; Masamura, Noriya; Shono, Jinji; Okamoto, Daisaku; Abe, Tomoko; Imai, Shinsuke

    2016-04-06

    The onion lachrymatory factor (LF) is produced from trans-S-1-propenyl-L-cysteine sulfoxide (PRENCSO) through successive reactions catalyzed by alliinase (EC 4.4.1.4) and lachrymatory factor synthase (LFS), and is responsible for the tear inducing-property and the pungency of fresh onions. We developed tearless, non-pungent onions non-transgenically by irradiating seeds with neon-ion at 20 Gy. The bulbs obtained from the irradiated seeds and their offspring bulbs produced by selfing were screened by organoleptic assessment of tear-inducing property or HPLC analysis of LF production. After repeated screening and seed production by selfing, two tearless, non-pungent bulbs were identified in the third generation (M3) bulbs. Twenty M4 bulbs obtained from each of them showed no tear-inducing property or pungency when evaluated by 20 sensory panelists. The LF production levels in these bulbs were approximately 7.5-fold lower than those of the normal onion. The low LF production levels were due to reduction in alliinase activity, which was a result of low alliinase mRNA expression (less than 1% of that in the normal onion) and consequent low amounts of the alliinase protein. These tearless, non-pungent onions should be welcomed by all who tear while chopping onions and those who work in facilities where fresh onions are processed.

  14. Commercial storage and marketing trials of irradiated Onions

    International Nuclear Information System (INIS)

    Nouchpramool, K.; Charoen, S.; Prachasitthisak, Y.

    1997-06-01

    Pilot scale storage tests were carried out in co-operation with commercial onions traders in the years 1986 and 1987 to evaluate the efficacy of irradiation for sprout inhibition of onions under actual commercial stored in commercial cold storage at 1-10 degrees C and 70-90% relative humidity. The results show that irradiation reduce sprouting in onions by 11 and 40 per cent and weight loss by 3 and 5 per cent after 5 and 6 months of storage, respectively. Storage losses are minimum when radiation is applied within two weeks of harvest. A maximum storage life of six months as against five months for controls is attained. Post cold storage life at ambient temperature for irradiated onions after withdrawal from cold storage is one week longer than that of non-irradiated controls. The radio inhibition process is technically feasible and economically justified as a profit can be made during the extended storage period. Marketing trials of irradiated onions conducted during and after termination of storage revealed no adverse comments from consumers and retailers/wholesalers. Wholesalers.retailers and consumers preferred irradiated onions because of their better physical quality and longer marketable life

  15. Persistence and bioaccumulation of oxyfluorfen residues in onion.

    Science.gov (United States)

    Sondhia, Shobha

    2010-03-01

    A field study was conducted to determine persistence and bioaccumulation of oxyflorfen residues in onion crop at two growth stages. Oxyfluorfen (23.5% EC) was sprayed at 250 and 500 g ai/ha on the crop (variety, N53). Mature onion and soil samples were collected at harvest. Green onion were collected at 55 days from each treated and control plot and analyzed for oxyfluorfen residues by a validated high-performance liquid chromatography method with an accepted recovery of 78-92% at the minimum detectable concentration of 0.003 microg g(-1). Analysis showed 0.015 and 0.005 microg g(-1) residues of oxyfluorfen at 250 g a.i. ha(-1) rate in green and mature onion samples, respectively; however, at 500 g a.i.ha(-1) rates, 0.025 and 0.011 microg g(-1) of oxyfluorfen residues were detected in green and mature onion samples, respectively. Soil samples collected at harvest showed 0.003 and 0.003 microg g(-1) of oxyfluorfen residues at the doses 250 and 500 g a.i. ha(-1), respectively. From the study, a pre-harvest interval of 118 days for onion crop after the herbicide application is suggested.

  16. An Onion Byproduct Affects Plasma Lipids in Healthy Rats

    DEFF Research Database (Denmark)

    Roldan-Marin, E.; Jensen, R. I.; Krath, Britta

    2010-01-01

    Onion may contribute to the health effects associated with high fruit and vegetable consumption. A considerable amount of onion production ends up as waste that might find use in foods. Onion byproduct has not yet been explored for potential health benefits. The aim of this study is to elucidate ...

  17. Pilot-scale studies on the irradiation of Bangladesh onions

    International Nuclear Information System (INIS)

    Matin, M.

    1986-01-01

    Two major varieties of onions were irradiated (50-80 Gy) and stored either at ambient temperature (20-37 deg. C) and humidity (70-90% RH) and/or under refrigeration (13-15 deg. C) for nine months. Raw and cooked irradiated onions had higher organoleptic ratings. Consumers found irradiated onions were of superior quality. There were no adverse comments on the sale and quality of irradiated onions purchased at competitive market prices. There were no adverse consumer comments nor negative reaction to purchase at competitive market prices

  18. Carbon Onions: Synthesis and Electrochemical Applications

    Energy Technology Data Exchange (ETDEWEB)

    McDonough, John K. [Drexel Univ., Philadelphia, PA (United States). Dept. of Materials Science and Engineering; Gogotsi, Y. [Drexel Univ., Philadelphia, PA (United States). Dept. of Materials Science and Engineering

    2013-01-01

    Onion-like carbon structures have been synthesized in many ways and large scale production is currently under study. The annealing method can satisfy the need for large scale production, though the ideal spherical shape is unachievable, and the temperature attainable in this method is not sufficient for treating the entire particle. The arc-discharge method provides an alternate pathway toward large scale synthesis. Due to its structure and electrochemical properties, carbon onions can be used as materials for electrochemical double layer capacitors (EDLC) and can be used to store energy across a much wider temperature range, which gives these materials advantages over conventional EDLCs. This and other aspects of carbon onions are discussed in this article.

  19. Monitoring of substrate and product concentrations in acetic fermentation processes for onion vinegar production by NIR spectroscopy: value addition to worthless onions.

    Science.gov (United States)

    González-Sáiz, J M; Esteban-Díez, I; Sánchez-Gallardo, C; Pizarro, C

    2008-08-01

    Wastes and by-products of the onion-processing industry pose an increasing disposal and environmental problem and represent a loss of valuable sources of nutrients. The present study focused on the production of vinegar from worthless onions as a potential valorisation route which could provide a viable solution to multiple disposal and environmental problems, simultaneously offering the possibility of converting waste materials into a useful food-grade product and of exploiting the unique properties and health benefits of onions. This study deals specifically with the second and definitive step of the onion vinegar production process: the efficient production of vinegar from onion waste by transforming onion ethanol, previously produced by alcoholic fermentation, into acetic acid via acetic fermentation. Near-infrared spectroscopy (NIRS), coupled with multivariate calibration methods, has been used to monitor the concentrations of both substrates and products in acetic fermentation. Separate partial least squares (PLS) regression models, correlating NIR spectral data of fermentation samples with each kinetic parameter studied, were developed. Wavelength selection was also performed applying the iterative predictor weighting-PLS (IPW-PLS) method in order to only consider significant spectral features in each model development to improve the quality of the final models constructed. Biomass, substrate (ethanol) and product (acetic acid) concentration were predicted in the acetic fermentation of onion alcohol with high accuracy using IPW-PLS models with a root-mean-square error of the residuals in external prediction (RMSEP) lower than 2.5% for both ethanol and acetic acid, and an RMSEP of 6.1% for total biomass concentration (a very satisfactory result considering the relatively low precision and accuracy associated with the reference method used for determining the latter). Thus, the simple and reliable calibration models proposed in this study suggest that they

  20. Regional and temporal variation in susceptibility to lambda-cyhalothrin in onion thrips, Thrips tabaci (Thysanoptera: Thripidae), in onion fields in New York.

    Science.gov (United States)

    Shelton, A M; Nault, B A; Plate, J; Zhao, J Z

    2003-12-01

    Populations of onion thrips, Thrips tabaci Lindeman, from commercial onion fields in New York were evaluated for their susceptibility to the commonly used pyrethroid, lambda-cyhalothrin (Warrior T), using a novel system called the Thrips Insecticide Bioassay System (TIBS). To use TIBS, thrips are collected directly from the plant into an insecticide-treated 0.5-ml microcentrifuge tube that has a flexible plastic cap with a small well into which 0.08 ml of a 10% sugar-water solution with food colorant is deposited. The solution is sealed into the well with a small piece of stretched parafilm through which the thrips can feed on the solution. Thrips mortality is assessed after 24 h with the help of a dissecting stereoscope. In 2001, onion thrips populations were collected from 16 different sites and resistance ratios were >1,000 in five populations. Percent mortality at 100 ppm, a recommended field rate, varied from 9 to 100%, indicating high levels of variation in susceptibility. Particular instances of resistance appeared to be the result of practices within an individual field rather than a regional phenomenon. In 2002, we also observed large differences in onion thrips susceptibility, not only between individual fields but also between thrips collected in a single field at mid season and late season, again suggesting that insecticide-use practices within an individual field caused differences in susceptibility. Additional tests indicated no differences in susceptibility between adult and larval onion thrips populations and only relatively minor differences between populations collected from different parts of the same field. Using TIBS, several populations of onion thrips with different susceptibilities to lambda-cyhalothrin were identified and then subjected to lambda-cyhalothrin-treated onion plants. There was a highly significant positive relationship between percent mortality of thrips from TIBS and percent mortality from the treated onion plants, indicating

  1. Pilot plant experiments for the sprout inhibition of onions by ionizing radiation

    International Nuclear Information System (INIS)

    Kalman, B.; Kiss, I.; Farkas, J.

    1978-01-01

    Experiments have been carried out with varieties grown from seed and sown onions, the former playing a decisive role in the onion production in Hungary. The results of pilot plant experiments proved the favourable and at the same time loss-decreasing effect of irradiation. During drying of onions treated and stored on large scale the yield-increasing effect of irradiation has been proved. In case of varieties grown from sown onions the saving of raw materials was almost 24 and 7%, respectively. In case of varieties grown from seed onions the yield-increase due to irradiation could not be observed each year. The decisive advantage of radiation treatment is direct yield-increase. However, the investigations of dried onions proved that the characteristics of the still not visible sprouts were affected favourably by irradiation, too. The consumer response to the irradiated onions has been favourable for several years. Though the consumers had a free choice, they distinctly insisted on buying irradiated onions on the basis of their favourable experience in using such onions. In order to utilize the results, an equipment has been designed for the economic operation of the onion-irradiating plants. (P.J.)

  2. Effect of Different Planting Methods of Onion (Allium cepa L. and Nitrogen Rate on Onion Growth Pattern under Interference with Purple Nutsedge (Cyperus rotundus

    Directory of Open Access Journals (Sweden)

    N Karimi Arpnahy

    2016-07-01

    Full Text Available Introduction Human always has looked for improving food production through increasing crops yield. In this path, weeds through competition with crop for environmental factors and inputs have reduced the quantity and quality of crop products. Competition for nitrogen absorption not only is the most common form of intra-specific competition amongst crop plants, but also is the most popular form of inter-specific competition in the system of weed-crop interference. Therefore, understanding the method of nitrogen absorption and its allocation in competing plants, will be a key tool to improve weed management strategies. Materials and Methods In order to study the effect of sowing method and nitrogen rate on the growth pattern of onion under interference with purple nutsedge, a factorial experiment based on a randomized complete block design was conducted with three replications at the Research Greenhouse of University of Birjand in 2013. The first factor included three sowing methods of onion (seed sowing, onion set and transplanting and the second factor consisted of three levels of nitrogen (50, 100 and 150 kg N ha-1, equivalent of 25, 50 and 75 mg N kg-1 soil that urea fertilizer with a purity of 46% was used for this purpose. Results and Discussion The results of the analysis of variance showed that nitrogen levels had significant effects on plant height, leaf area index as well as aboveground and bulb dry weights. Furthermore, sowing methods revealed significant effects on plant height, leaf number, leaf area index as well as aboveground and bulb dry weights. Moreover, the interaction between sowing methods and levels of nitrogen had a significant effect on plant height, leaf area index and aboveground dry weight, while it had no significant effect on leaf number and bulb dry weight. The results of the comparisons of the means of onion planting methods and nitrogen levels interactions confirmed that the superiority of the influence of onion set

  3. Weed infestation of onion in soil reduced cultivation system

    Directory of Open Access Journals (Sweden)

    Marzena Błażej-Woźniak

    2013-12-01

    Full Text Available Field experiment was conducted in the years 1998-2000 in GD Felin. The influence of no-tillage cultivation and conventional tillage with spring ploughing on weed infestation of onion was compared. In experiment four cover crop mulches (Sinapis alba L., Vicia sativa L., Phacelia tanacetifolia B., Avena sativa L. were applied. From annual weeds in weed infestation of onion in great number Matricaria chamomilla L., and Senecio vulgaris L. stepped out. and from perennial - Agropyron repens (L.P.B. Reduced soil cultivation system (no-tillage caused the significant growth of primary weed infestation of onion in comparison with conventional tillage. In all years of investigations the executed pre-sowing ploughing limited significantly the annual weeds' number in primary weed infestation. The applied mulches from cover plants limited in considerable degree the number of primary weed infestation. In all years of investigations the most weeds stepped out on control object. Among investigated cover crop mulches Vicia sativa L. and Avena sativa L. had a profitable effect on decrease of onion`s primary weed infestation. Soil cultivation system and cover crop mulches had no signi ficant residual influence on the secondary weed infestation of onion.

  4. Flame synthesis of carbon nano onions using liquefied petroleum gas without catalyst

    International Nuclear Information System (INIS)

    Dhand, Vivek; Prasad, J. Sarada; Rao, M. Venkateswara; Bharadwaj, S.; Anjaneyulu, Y.; Jain, Pawan Kumar

    2013-01-01

    Densely agglomerated, high specific surface area carbon nano onions with diameter of 30–40 nm have been synthesized. Liquefied petroleum gas and air mixtures produced carbon nano onions in diffusion flames without catalyst. The optimized oxidant to fuel ratio which produces carbon nano onions has been found to be 0.1 slpm/slpm. The experiment yielded 70% pure carbon nano onions with a rate of 5 g/h. X-ray diffraction, high-resolution electron microscopy and Raman spectrum reveal the densely packed sp 2 hybridized carbon with (002) semi-crystalline hexagonal graphite reflection. The carbon nano onions are thermally stable up to 600 °C. - Highlights: ►Flame synthesized carbon nano onions with 30–40 nm diameters. ►LPG/air, diffusion type flame used in absence of catalyst to produce nano onions. ►Carbon nano onion production rate is 5 g/hr and with 70% purity.

  5. Evaluating the suitability of maggot meal as a partial substitute of soya bean on the productive traits, digestibility indices and organoleptic properties of broiler meat.

    Science.gov (United States)

    Khan, S; Khan, R U; Sultan, A; Khan, M; Hayat, S U; Shahid, M S

    2016-08-01

    This study was conducted to replace soya bean meal with maggot meal at difference percentages. A total of 120 one-day-old broiler chicks were divided into four groups. Four diets were formulated with maggot meal to replace soya bean meal at the rate of 0%, 10%, 20% and 30% designated as A, B, C and D respectively. Feed and water were offered ad libitum during the study which lasted for 28 days. Feed intake decreased significantly in treated groups while body weight, Feed conversion ratio and dressing percentage increased significantly in group D. Apparent metabolizable energy increased significantly in the group fed 30% maggot meal; however, no significant difference was found in dry matter, crude protein, crude fibre, ether extract, ash and nitrogen free extract between the control and treated groups. The result of sensory evaluation revealed that tenderness and juiciness increased significantly in group C and B respectively. The colour and flavour were most accepted in group B and C. The results indicate that replacement of soya bean meal at the rate of 30% in broiler feed produced most favourable results. Journal of Animal Physiology and Animal Nutrition © 2016 Blackwell Verlag GmbH.

  6. Flame synthesis of carbon nano onions using liquefied petroleum gas without catalyst

    Energy Technology Data Exchange (ETDEWEB)

    Dhand, Vivek, E-mail: vivekdhand2012@gmail.com [Centre for Knowledge Management of Nanoscience and Technology, 12-5-32/8, Vijayapuri Colony, Tarnaka, Secunderabad-500 017, A.P (India); Prasad, J. Sarada; Rao, M. Venkateswara [Centre for Environment, Institute of Science and Technology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad 500 085 (India); Bharadwaj, S. [Department of Physics, CVR College of Engineering and Osmania University, Hyderabad 501510, A.P (India); Anjaneyulu, Y. [TLGVRC, Jackson State University, JSU Box 18739, Jackson, MS 39217-0939 (United States); Jain, Pawan Kumar [International Advanced Research Centre for Powder Metallurgy and New Materials (ARCI), Balapur PO, Hyderabad 500005, Andhra Pradesh (India)

    2013-03-01

    Densely agglomerated, high specific surface area carbon nano onions with diameter of 30-40 nm have been synthesized. Liquefied petroleum gas and air mixtures produced carbon nano onions in diffusion flames without catalyst. The optimized oxidant to fuel ratio which produces carbon nano onions has been found to be 0.1 slpm/slpm. The experiment yielded 70% pure carbon nano onions with a rate of 5 g/h. X-ray diffraction, high-resolution electron microscopy and Raman spectrum reveal the densely packed sp{sup 2} hybridized carbon with (002) semi-crystalline hexagonal graphite reflection. The carbon nano onions are thermally stable up to 600 Degree-Sign C. - Highlights: Black-Right-Pointing-Pointer Flame synthesized carbon nano onions with 30-40 nm diameters. Black-Right-Pointing-Pointer LPG/air, diffusion type flame used in absence of catalyst to produce nano onions. Black-Right-Pointing-Pointer Carbon nano onion production rate is 5 g/hr and with 70% purity.

  7. Detection of Argentine onions treated with 60 Co irradiation

    International Nuclear Information System (INIS)

    Romanelli, Maria Fernanda; Sebastiao, Katia I.; Leite, Quilma R.; Koseki, Paula M.; Hamasaki, Koji; Villavicencio, Anna Lucia H.; Croci, Clara

    2000-01-01

    Brazil has been the most important MERCOSUL's purchaser of fresh onions from Argentina. The increased claim for this fresh product has forced a consensus between the members nations, as regards to phytosanitary restrictions. The radio inhibition is described on National Food Codes in Brazil and Argentina. Methods of food irradiation detection must be performed, since they increase the consumer confidence. Quick and simple screening tests indicate whether a food product has been irradiated or not. This present study verified the DNA fragments of argentine fresh onions, produced during radiation process and 6 months of storage period. The DNA fragments are analyzed for detection of irradiated foods. The irradiated onions presented extensive DNA migrations, as comets, when submitted to agarose gel electrophoresis. They also showed more shelf life compared to the unirradiated onions. The unirradiated samples exhibited only limited DNA migration. This initial screen method showed to be effective for detection of irradiated onions. (author)

  8. An onion byproduct affects plasma lipids in healthy rats.

    Science.gov (United States)

    Roldán-Marín, Eduvigis; Jensen, Runa I; Krath, Britta N; Kristensen, Mette; Poulsen, Morten; Cano, M Pilar; Sánchez-Moreno, Concepción; Dragsted, Lars O

    2010-05-12

    Onion may contribute to the health effects associated with high fruit and vegetable consumption. A considerable amount of onion production ends up as waste that might find use in foods. Onion byproduct has not yet been explored for potential health benefits. The aim of this study is to elucidate the safety and potential role of onion byproducts in affecting risk markers of cardiovascular disease (CVD). For that purpose, the effects of an onion byproduct, Allium cepa L. cepa 'Recas' (OBP), and its two derived fractions, an ethanolic extract (OE) and a residue (OR), on the distribution of plasma lipids and on factors affecting cholesterol metabolism in healthy rats have been investigated. The OBP or its fractions did not significantly reduce cholesterol or down-regulate hepatic 3-hydroxy-3-methylglutaryl-coenzyme A reductase (Hmgcr) gene expression. The OR even had the effect of increasing plasma triacylglycerides (TAG) and cholesterol in the very low density lipoprotein (VLDL-C) fraction. Neither total bile acids nor total primary or secondary bile acids were significantly affected by feeding rats the OBP or its fractions. Principal component analysis combining all markers revealed that the controls could be completely separated from OBP, OE, and OR groups in the scores plot and also that OE and OR groups were separated. Plasma lipids and bile acid excretion were the discriminating loading factors for separating OE and OR but also contributed to the separation of onion-fed animals and controls. It was concluded that the onion byproduct did not present significant beneficial effects on individual markers related to plasma lipid transport in this healthy rat model but that onion byproduct contains factors with the ability to modulate plasma lipids and lipoprotein levels.

  9. Relationship Between Consumer Acceptability and Pungency-Related Flavor Compounds of Vidalia Onions.

    Science.gov (United States)

    Kim, Ha-Yeon; Jackson, Daniel; Adhikari, Koushik; Riner, Cliff; Sanchez-Brambila, Gabriela

    2017-10-01

    A consumer study was conducted to evaluate preferences in Vidalia onions, and define consumer acceptability thresholds for commonly analyzed flavor compounds associated with pungency. Two varieties of Vidalia onions (Plethora and Sapelo Sweet) were grown at 3 fertilizer application rates (37.5 and 0; 134.5 and 59.4; and 190 and 118.8 kg/ha of nitrogen and sulfur, respectively), creating 6 treatments with various flavor attributes to use in the study. Bulb soluble solids, sugars, pyruvic acid, lachrymatory factor (LF; propanethial S-oxide), and methyl thiosulfinate (MT) content were determined and compared to sensory responses for overall liking, intensity of the sharp/pungent/burning sensation (SPB), and intent to buy provided by 142 consumers. Onion pyruvate, LF, MT, and sugar content increased as fertilization rate increased, regardless of onion variety. Consumer responses showed participants preferred onions with low SPB, which correlated positively to lower pyruvate, LF and MT concentrations, but showed no relationship to total sugars in the onion bulb. Regression analyses revealed that the majority of consumers (≥55%) found the flavor of Vidalia onions acceptable when the concentrations of LF, pyruvic acid, and MT within the bulbs were below 2.21, 4.83, and 0.43 nmol/mL, respectively. These values will support future studies aimed at identifying the optimal cultivation practices for production of sweet Vidalia onions, and can serve as an industry benchmark for quality control, thus ensuring the flavor of Vidalia onions will be acceptable to the majority of consumers. This study identified the relationship between consumer preferences and commonly analyzed flavor compounds in Vidalia onions, and established thresholds for these compounds at concentrations which the majority of consumers will find desirable. These relationships and thresholds will support future research investigating how cultural practices impact onion quality, and can be used to assist

  10. Gamma irradiation of onions and garlic

    International Nuclear Information System (INIS)

    Baraldi, D.

    1975-01-01

    Technological and economic feasibility of gamma irradiation of onions and garlic on an industrial scale are studied. Statistical data on production, consumption, exportation and losses during storage are analyzed. Traditional methods of food preservation are reviewed and gamma irradiation techniques are presented as an alternative to sprout inhibition. Requirements for the irradiation of onions and garlic on a commercial scale including a cost benefit analysis are discussed. Some conclusions are formulated on licensing and prospects

  11. 2-undecanone and 2-tridecanone in field-grown onion.

    Science.gov (United States)

    Antonious, George F

    2013-01-01

    A field study was conducted to investigate the impact of soil amendments on concentrations of two volatile organic compounds, 2-undecanone and 2-tridecanone, in onion bulbs. The soil in five plots was mixed with sewage sludge, five plots were mixed with yard waste compost, five plots were mixed with laying hen manure each at 15 t acre(-1), and five unamended plots that never received soil amendments were used for comparison purposes. Plots (n = 20) were planted with onion, Allium cepa L. var. Super Star-F1 bulbs. Gas chromatographic/mass spetrometric (GC/MS) analyses of mature onion bulbs crude extracts revealed the presence of two major fragment ions that correspond to 2-undecanone and 2-tridecanone. Soil amended with yard waste compost enhanced 2-undecanone and 2-tridecanone production by 31 and 59%, respectively. Soil amended with chicken manure enhanced 2-undecanone and 2-tridecanone production by 28 and 43%, respectively. Concentrations of 2-undecanone and 2-tridecanone were lowest in onion bulbs of plants grown in sewage sludge and unamended soil, respectively. The increased concentrations of 2-undecanone and 2-tridecanone in onion bulbs may provide a protective character against insect and spider mite attack in field grown onions.

  12. Weeds of onion fields and effects of some herbicides on weeds in ...

    African Journals Online (AJOL)

    Weeds are one of the most important problems in onion (Allium cepa L.) production areas, since onion plants are poor competitors. This study was conducted in order to identify the weed species in onion fields in Cukurova Region, establish the effects of some herbicides on weeds and the yield of onion in reducing the ...

  13. Structural analyses and immunomodulatory properties of fructo-oligosaccharides from onion (Allium cepa).

    Science.gov (United States)

    Kumar, V Prasanna; Prashanth, K V Harish; Venkatesh, Y P

    2015-03-06

    Onion (Allium cepa) is an immune-boosting food rich in fructans. The major aim of this study is to characterize and investigate the immunomodulatory properties of onion fructo-oligosaccharides (FOS). FOS was isolated from onion bulbs by hot 80% ethanol extraction (yield: ∼4.5 g/100 g fw) followed by gel permeation chromatography. NMR of onion FOS revealed unusual β-D-Glc terminal residue at the non-reducing end. TLC and ESI-MS analyses showed that onion FOS ranged from trisaccharides to hexasaccharides. Onion FOS (50 μg/mL) significantly increased (∼3-fold) the proliferation of mouse splenocytes/thymocytes vs. control. Further, onion FOS enhanced (∼2.5-fold) the production of nitric oxide by peritoneal exudates cells (PECs) from Wistar rats; intracellular free radicals production and phagocytic activity of isolated murine PECs were also augmented. Our structural and in vitro results indicate that onion FOS comprising of tri- to hexasaccharide units belongs to inulin-type fructans, and possess immunostimulatory activities towards murine lymphocytes and macrophages. Copyright © 2014 Elsevier Ltd. All rights reserved.

  14. Sanitation of onion powder by irradiation and heating

    Energy Technology Data Exchange (ETDEWEB)

    Delkinova, S M [Bulgarplod, Plovdiv (Bulgaria); Dupuy, P [Institut National de Recherches Agronomiques (INRA), 21 - Dijon (France). Station de Recherches sur les Aliments de l' Homme

    1973-01-01

    Onion powder is frequently contaminated by a mesophile bacterial flora containing some spore-forming strains. Heating is not an admissible method of sterilization in this case, because of the volatile aromatic substances formed by the enzyme system present. After irradiation with 0.8 Mrad the surviving microbial flora contained Clostridium, Bacillus, Enterococcus and Micrococcus strains. These Micrococcus strains are less resistant than M.radiodurans and do not develop as spoilage organisms in foods. During the months following irradiation no growth was observed, on the contrary the number of cells surviving diminished in the same way both in the irradiated and the untreated samples. In soup powders with short cooking time, if the irradiated or untreated onion powder is added after boiling, a chance is caused by the growth of Lactobacillus or Bacillus strains. A radiation treatment with 0.4 Mrad gives a sterile soup, when boiled for 5 minutes. In meat pastes the most dangerous microbial flora is that consisting mainly of spora-forming anaerobic bacteria. It was shown that if the pasta was aromatized with onion powder treated with 0.2 Mrad before cooking, no growth occurred. The control sample containing untreated onion powder was spoiled by a microbial flora dominated by spore-forming aerobic bacteria. These two experiments proved that onion powder can be decontaminated by treatment with 0.2 or 0.4 Mrad. It is known that this dose has no detrimental effect on the organoleptic quality of the onion powder and the cost of irradiation is compatible with the price of the product treated. (F.J.)

  15. Growth and yield of onion as influenced by organic and inorganic ...

    African Journals Online (AJOL)

    Growth and yield of onion as influenced by organic and inorganic fertilizer in ... the effect of NPK 15:15:15 and poultry manure on the growth and yield of onion plant (Allium cepa L.). ... Keywords: onion, manure, harvest index, crop growth rate, ...

  16. Inhibition of platelet activation by lachrymatory factor synthase (LFS)-silenced (tearless) onion juice.

    Science.gov (United States)

    Thomson, Susan J; Rippon, Paula; Butts, Chrissie; Olsen, Sarah; Shaw, Martin; Joyce, Nigel I; Eady, Colin C

    2013-11-06

    Onion and garlic are renowned for their roles as functional foods. The health benefits of garlic are attributed to di-2-propenyl thiosulfinate (allicin), a sulfur compound found in disrupted garlic but not found in disrupted onion. Recently, onions have been grown with repressed lachrymatory factor synthase (LFS) activity, which causes these onions to produce increased amounts of di-1-propenyl thiosulfinate, an isomer of allicin. This investigation into the key health attributes of LFS-silenced (tearless) onions demonstrates that they have some attributes more similar to garlic and that this is likely due to the production of novel thiosulfinate or metabolites. The key finding was that collagen-induced in vitro platelet aggregation was significantly reduced by tearless onion extract over normal onion extract. Thiosulfinate or derived compounds were shown not to be responsible for the observed changes in the inflammatory response of AGS (stomach adenocarcinoma) cells to tumor necrosis factor alpha (TNFα) when pretreated with model onion juices. A preliminary rat feeding trial indicated that the tearless onions may also play a key role in reducing weight gain.

  17. Onion root tip cell system for biodosimetry?

    International Nuclear Information System (INIS)

    Paradiz, J; Druskovic, B.; Lovka, M.; Skrk, J.

    1996-01-01

    Methodology for radiation dose assessment based on chromosomal damage to plant cells has no yet been established, although root meristems have been the pioneer cytogenetic materials and profound analyses of irradiated meristematic cells of horse bean (Viciafaba L.) had been performed. Onion (Allium cepa L.) root tips frequently used for radiation cytogenetic studies, are recently considered to be one of the most promising plant test system for the detection of genotoxic environmental pollutants. We studied the possibility of using cytogenetic analyses of irradiated onion cells to determine the effective biological dose of ionizing radiation. The dose-effect relationships for chromosomal damages to onion meristematic cells were established after plants had been irradiated and subsequently grown in both laboratory and field conditions

  18. Low hybrid onion seed yields relate to honey bee visits and insecticide use

    Directory of Open Access Journals (Sweden)

    Rachael Long

    2011-07-01

    Full Text Available Onion thrips, previously considered of minor importance to hybrid onion seed production in California, vector the newly introduced iris yellow spot virus, a serious pathogen of onions that can cause significant yield losses. Insecticide use to control onion thrips has increased in onion seed fields, coincident with a steep decrease in yields, especially in Colusa County. We examined a number of possible contributing factors and found a strong positive correlation between honey bee activity and onion seed set, indicating that a lack of pollination may be contributing to the reduced yields. In addition, honey bee visits to onion flowers were negatively correlated with the number of insecticides applied per field and field size. Reduced onion seed yields in recent years could be associated with the increase in insecticide use, which may be repelling or killing honey bees, important pollinators of this crop.

  19. Anti-Obesity Effects of Onion Extract in Zucker Diabetic Fatty Rats

    Directory of Open Access Journals (Sweden)

    Kiharu Igarashi

    2012-10-01

    Full Text Available Anti-obesity effects of onion extract were determined in obesity and diabetes-prone Zucker diabetic fatty rats by measuring the efficacy of markers concerned with diabetes and obesity. Body and adipose tissue weights in 5% of onion extract-fed group were found to be significantly lower than the control group without onion extract. Fasting blood glucose and HOMA-IR levels were also improved, although the serum insulin and leptin levels did not show any remarkable difference. Serum triglyceride and free fatty acid levels in both the 3% and 5%-fed group were found to be reduced compared to the control group. Additionally the feeding of the onion extract increased the glucose tolerance. These results suggest that dietary onion extract is beneficial for improving diabetes by decreasing lipid levels. We also examined differentiation ability of rat white preadipocyte cells using the onion extract and its sulfur-containing components. Cycloalliin, S-methyl-l-cysteine, S-propyl-l-cysteine sulfoxide, dimethyl trisulfide, especially S-methyl-l-cysteine sulfoxide were reported to be effective in inhibiting formation of oil drop in the cells, suggesting that these compounds may be involved in the anti-obesity effect of the onion extract.

  20. 78 FR 23671 - Onions Grown in South Texas; Increased Assessment Rate

    Science.gov (United States)

    2013-04-22

    ... behalf. There are approximately 85 producers of onions in the production area and approximately 30... FR] Onions Grown in South Texas; Increased Assessment Rate AGENCY: Agricultural Marketing Service... Texas Onion Committee (Committee) for the 2012-13 and subsequent fiscal periods from $0.025 to $0.03 per...

  1. Low hybrid onion seed yields relate to honey bee visits and insecticide use

    OpenAIRE

    Long, Rachael Freeman; Morandin, Lora

    2011-01-01

    Onion thrips, previously considered of minor importance to hybrid onion seed production in California, vector the newly introduced iris yellow spot virus, a serious pathogen of onions that can cause significant yield losses. Insecticide use to control onion thrips has increased in onion seed fields, coincident with a steep decrease in yields, especially in Colusa County. We examined a number of possible contributing factors and found a strong positive correlation between honey bee activity an...

  2. Semi-Continuous Fermentation of Onion Vinegar and Its Functional Properties.

    Science.gov (United States)

    Lee, Sulhee; Lee, Jin-A; Park, Gwi-Gun; Jang, Jae-Kweon; Park, Young-Seo

    2017-08-08

    For the fermentation of vinegar using onion, acetic acid bacteria and yeast strains with high fermentation ability were screened. Among them, Saccharomyces cerevisiae 1026 was selected as a starter for ethanol production and Acetobacter orientalis MAK88 was selected as a vinegar producer. When the two-stage fermentation of onion vinegar was performed at 28 °C, the titratable acidity reached 4.80% at 24 h of fermentation. When semi-continuous fermentation proceeded to charge-discharge consisting of three cycles, the acetic acid content reached 4.35% at 48 h of fermentation. At this stage, the fermentation efficiency, acetic acid productivity, and specific product formation rate were 76.71%, 17.73 g/(L·d), and 20.58 g/(g·h), respectively. The process in this study significantly reduced the fermentation time and simplified the vinegar production process. The content of total flavonoids and total polyphenols in onion vinegar were 104.36 and 455.41 μg/mL, respectively. The antioxidant activities of onion vinegar in terms of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic) acid (ABTS⁺) radical scavenging activity, and reducing power were 75.33%, 98.88%, and 1.28, respectively. The nitrite scavenging abilities of onion vinegar were 95.38 at pH 1.2. The onion vinegar produced in this study showed higher organoleptic acceptability than commercial onion vinegar.

  3. Facilitating trade in irradiated onions in the Philippines

    International Nuclear Information System (INIS)

    Lustre, A.O.; Vito, F.R.; Manalastas, Z.I.

    2001-01-01

    The irradiation of large volumes of three varieties of onions (56 tons of red creole, 20 tons of yellow granex and 14 tons of shallots) at the Philippine Nuclear Research Institute, was carried out by the private sector (National Onion Growers Marketing Cooperative) with the technical assistance of government. The data confirmed increased profits from sale of irradiated onions due to the presence of significantly higher percentages of good bulbs after storage (26%, 36% and 69% more for the yellow granex, red creole and shallot varieties respectively) due to inhibition of sprouting. Industry's acceptance of the process was indicated by the large volume of onions irradiated during the period (96 tons) and the application of the treatment to shallots for the first time. In spite of the significant benefits, the high capital cost of the plant did not justify its operation solely for onions. The opening of markets for other applications was considered essential. The percentage of consumers willing to purchase irradiated food was low but this increased from 15% to 79% when appropriate information on the technology was provided to them. It was concluded that the provision of information is the key to consumer acceptance. However, the opening of profitable markets in order to achieve economic feasibility is the key to the commercialization of the technology. (author)

  4. Bio-ethanol Production from Green Onion by Yeast in Repeated Batch.

    Science.gov (United States)

    Robati, Reza

    2013-09-01

    Considered to be the cleanest liquid fuel, bio-ethanol can be a reliable alternative to fossil fuels. It is produced by fermentation of sugar components of plant materials. The common onions are considered to be a favorable source of fermentation products as they have high sugar contents as well as contain various nutrients. This study focused on the effective production of ethanol from Green onion (Allium fistulosum L.) by the yeast "Saccharomyces cerevisiae" in repeated batch. The results showed that the total sugar concentration of onion juice was 68.4 g/l. The maximum rate of productivity, ethanol yield and final bio-ethanol percentage was 7 g/l/h (g ethanol per liter of onion juice per hour), 35 g/l (g ethanol per liter of onion juice) and 90 %, respectively.

  5. Strain distributions in nano-onions with uniform and non-uniform compositions

    International Nuclear Information System (INIS)

    Duan, H L; Karihaloo, B L; Wang, J; Yi, X

    2006-01-01

    Nano-onions are ellipsoidal or spherical particles consisting of a core surrounded by concentric shells of nanometre size. Nano-onions produced by self-assembly and colloidal techniques have different structures and compositions, and thus differ in the state of strains. The mismatch of the thermal expansion coefficients and lattice constants between neighbouring shells induces stress/strain fields in the core and shells, which in turn affect their physical/mechanical properties and/or the properties of the composites containing them. In this paper, the strains in embedded and free-standing nano-onions with uniform and non-uniform compositions are studied in detail. It is found that the strains in the nano-onions can be modified by adjusting their compositions and structures. The results are useful for the band structure engineering of semiconductor nano-onions

  6. Vinegar as a broadcast herbicide for spring-transplanted onions

    Science.gov (United States)

    The weed control challenges for onion production are formidable; however, these challenges are even greater for those considering organic crop production. Organic onion producers need additional organic herbicides that can effectively provide post-emergent weed control. Field research was conducted...

  7. 78 FR 28118 - Vidalia Onions Grown in Georgia; Change in Reporting and Assessment Requirements

    Science.gov (United States)

    2013-05-14

    ... onion producers in the designated production area. Small agricultural service firms, which include...; FV13-955-1 IR] Vidalia Onions Grown in Georgia; Change in Reporting and Assessment Requirements AGENCY... Vidalia onions grown in Georgia (order). The order regulates the handling of Vidalia onions grown in...

  8. Control of Aspergillus niger with garlic, onion and leek extracts

    African Journals Online (AJOL)

    Administrator

    2007-02-19

    Feb 19, 2007 ... Key words: Aspergillus niger, garlic, onion, leek, antifungal activity. ..... Antimicrobial activity of essential oil extracts of various onions (Allium cepa) ... activity of oregano and thyme essential oils applied as fumigants against.

  9. Effects of fistular onion stalk extract on the level of NO and ...

    African Journals Online (AJOL)

    user

    2011-03-28

    Mar 28, 2011 ... In this study, we investigated the effects of fistular onion stalk extracts on nitric oxide ... and flavonoids in onion (Knekt et al., 1996). ... cells lie between blood flow and smooth muscle cells. ... strated by a reduction in the blood vessel dilation factors .... raw onion inhibits platelet-mediated thrombosis in dogs.

  10. Corrosion Inhibitor of Carbon Steel from Onion Peel Extract

    Directory of Open Access Journals (Sweden)

    Muhammad Samsudin Asep

    2018-01-01

    Full Text Available Carbon steels composed by two main elements, they are iron (Fe and carbon (C elements which widely used in industrial because of its resistance and more affordable than stainless steel, but their weakness is they have low corrosion resistance. One way to modify carbon steel is by coating them with antioxidant compounds that can delay, slow down, and prevent lipid oxidation process, which obtained from onion peel extract. Several studies on corrosion inhibitors have been performed. However, the efficiency was not reach the optimum. This study aims to examine the effect of onion peel extract concentration on the efficiency of corrosion inhibitor and characterization of the green corrosion inhibitor from onion peel extract. This research method begins by extracting onion peel to 200 ml solvent which we use aquadest and methanol and mixed with 5 grams of crushed onion peel, then let them be extracted for 60 minutes with room temperature. Once it was filtered and the solution obtained, followed by evaporating process with rotary evaporator to decrease the content of solvent. The product is ready to be used as a green corrosion inhibitor of carbon steel in 1 mol/L HCl. While the analysis used is HPLC qualitative analysis, and electroplatting process. The impedance is measured at a frequency of 100 kHz to 4 mHz with an AC current of 10mV. Inhibitor concentrations are vary between 2 ml and 4 ml of onion peel extract. Electroplatting is done within 30 minutes with 10 minutes each checking time. Furthermore, quantitative analysis was done for the analysis of corrosion rate and weight loss. Based on HPLC analysis, it is known that the extract of onion peel contains 1mg/L of quercetin, which is belong to flavonoid group as green inhibitor. While electroplatting process, aquadest solvent having average efficiency of 99,57% for 2 ml of extract, and 99,60% for 4 ml of extract. Methanol solvent having average efficiency of 99,52% for 2 ml of extract and 99

  11. Gamma radiation effects on different sorts of onions

    International Nuclear Information System (INIS)

    Burov, B.A.

    1977-01-01

    Gamma radiation effects on different sorts of onions were studied to improve ways of obtaining agricultural vegetation mutations and to find out the genotype role in induced mutagenesis. It is established that rhizome onion seeds are more radiosensitive than bulbous ones, ephemeroide form seeds are most stable among bulbous plants. Table data on dependence of seed germination and plant survival on radiation dose are presented

  12. MEDEIA, LADY MACBETH, DELIA E TRAMELL: APROXIMAÇÕES INTERARTES SOB A FIGURA DA BRUXA

    Directory of Open Access Journals (Sweden)

    Márcio Henrique Muraca

    2014-10-01

    Full Text Available O artigo pretende aproximar a figura popular da bruxa ao mito da mulher funesta, símbolo da ameaça da ordem política pela perturbação da consciência moral de uma época. Além da crença da bruxaria e dos mitos em torno da mulher, é possível chegar ao embate entre público e privado, político e psicológico. Quatro personagens femininas consagradas ilustram a tese que aqui se pretende: Medeia (Eurípedes – teatro grego, Lady Macbeth (Shakespeare – teatro moderno, Delia (Cortázar – literatura latino-americana, séc. XX e Catherine Tramell (Osborne – obra fílmica blockbuster e livro baseado no script. O princípio teórico norteador deste estudo é o das relações de poder, de Michel Foucault, sobretudo por meio da obra Microfísica do Poder. Quanto à questão da bruxa/bruxaria, o artigo vale-se de Pensando em Demônios, de Stuart Clark.

  13. Profitability And Resource Use Efficiency In Dry Season Onion ...

    African Journals Online (AJOL)

    The study evaluated profitability and resource use efficiency in dry season onion production in Sokoto and Kebbi States. Eight local government areas (L. G. As), four from each State, were purposively selected for the study. From each L.G.A., four villages were selected and from each village, five dry season onion farmers ...

  14. Facile synthesis of water-soluble carbon nano-onions under alkaline conditions

    Directory of Open Access Journals (Sweden)

    Gaber Hashem Gaber Ahmed

    2016-05-01

    Full Text Available Carbonization of tomatoes at 240 °C using 30% (w/v NaOH as catalyst produced carbon onions (C-onions, while solely carbon dots (C-dots were obtained at the same temperature in the absence of the catalyst. Other natural materials, such as carrots and tree leaves (acer saccharum, under the same temperature and alkaline conditions did not produce carbon onions. XRD, FTIR, HRTEM, UV–vis spectroscopy, and photoluminescence analyses were performed to characterize the as-synthesized carbon nanomaterials. Preliminary tests demonstrate a capability of the versatile materials for chemical sensing of metal ions. The high content of lycopene in tomatoes may explain the formation of C-onions in alkaline media and a possible formation mechanism for such structures was outlined.

  15. Effects of an onion by-product on bioactivity and safety markers in healthy rats.

    Science.gov (United States)

    Roldán-Marín, Eduvigis; Krath, Britta N; Poulsen, Morten; Binderup, Mona-Lise; Nielsen, Tom H; Hansen, Max; Barri, Thaer; Langkilde, Søren; Cano, M Pilar; Sánchez-Moreno, Concepción; Dragsted, Lars O

    2009-12-01

    Onions are excellent sources of bioactive compounds including fructo-oligosaccharides (FOS) and polyphenols. An onion by-product was characterised in order to be developed as a potentially bioactive food ingredient. Our main aim was to investigate whether the potential health and safety effects of this onion by-product were shared by either of two derived fractions, an extract containing the onion FOS and polyphenols and a residue fraction containing mainly cell wall materials. We report here on the effects of feeding these products on markers of potential toxicity, protective enzymes and gut environment in healthy rats. Rats were fed during 4 weeks with a diet containing the products or a control feed balanced in carbohydrate. The onion by-product and the extract caused anaemia as expected in rodents for Allium products. No other toxicity was observed, including genotoxicity. Glutathione reductase (GR) and glutathione peroxidase (GPx1) activities in erythrocytes increased when rats were fed with the onion extract. Hepatic gene expression of Gr, Gpx1, catalase, 5-aminolevulinate synthase and NAD(P)H:quinone oxidoreductase was not altered in any group of the onion fed rats. By contrast, gamma-glutamate cysteine ligase catalytic subunit gene expression was upregulated but only in rats given the onion residue. The onion by-products as well as the soluble and insoluble fractions had prebiotic effects as evidenced by decreased pH, increased butyrate production and altered gut microbiota enzyme activities. In conclusion, the onion by-products have no in vivo genotoxicity, may support in vivo antioxidative defence and alter the functionality of the rat gut microbiota.

  16. Effect of different exposed lights on quercetin and quercetin glucoside content in onion (Allium cepa L.).

    Science.gov (United States)

    Ko, Eun Young; Nile, Shivraj Hariram; Sharma, Kavita; Li, Guan Hao; Park, Se Won

    2015-07-01

    Quercetin and quercetin glucosides are the major flavonols present in onion (Allium cepa L.) and are predominantly present as quercetin, quercetin-3,4'-diglucoside and quercetin-4'-glucoside. Effect of different light wavelengths on onion after harvest and storage, with fluorescent, blue, red and ultra violet light influenced the quercetin and quercetin glucosides profile. In a peeled onion, all the light treatments elevated quercetin content in bulb. Among them, particularly fluorescent light effect was more eminent which stimulates the maximum synthesis of quercetin in onion. In case of whole onion bulb, skin and pulp showed different responses to light treatment, respectively. The pulp had the highest quercetin glucosides under blue light, whereas the lowest under fluorescent light. Onion skin showed nearly opposite pattern as compared to the pulp. In particular, light treatment proved to be a better way to increase the level of quercetin content in onions which might be utilized for industrial production of bioactive compounds from onion and onion waste products.

  17. SE-ENRICHMENT OF CARROT AND ONION VIA FOLIAR APPLICATION

    OpenAIRE

    Kapolna, Emese; Laursen, Kristian H.; Hillestrøm, Peter; Husted, Søren; Larsen, Erik H.

    2008-01-01

    The aim of this work was to study the selenium accumulation in carrot and onion plants using foliar application by sodium selenite and sodium selenate. Furthermore, we aimed at identifying the Se species biosynthesised by onion and carrot plants. The results were used to prepare for production of 77Se enriched plants for an ongoing human absorption study.

  18. POST-HARVEST QUALITY OF ONION 'ATACAMA' PRODUCED WITH DIFFERENT LEVELS OF NITROGEN

    Directory of Open Access Journals (Sweden)

    Acácio Figueiredo Neto

    2014-07-01

    Full Text Available The onion is an economically important crop for Brazil and its production is increasing in several regions of the country. The Northeast region uses onion seeds of lower quality than other regions of Brazil, and this causes lower production. The region of the São Francisco Valley is considered of great agricultural potential and many companies are investing in agriculture. So, researchers develop varieties of onions to suit the conditions of the semi-arid northeast. This study aimed to evaluate the physical and chemical conditions of the onion híbrida ‘Atacama’, produced at the experimental station of EMBRAPA in Mandacaru, Juazeiro-BA. The experiments were performed on the storage of agricultural products of the College of Agricultural and Environmental Engineering UNIVASF, where it was observed that the bulbs stored at refrigeration temperature have lower mass loss laboratory than fruits stored at room temperature. Onion color after 30 days storage darkens and the steadiness decreases as the fruit is stored. There were no variations in the quality of post- harvest onion by varying the dosage of 120 kg/ha and 70 kg/ha of nitrogen.

  19. Interactions involving ozone, Botrytis cinerea, and B. squamosa on onion leaves

    Energy Technology Data Exchange (ETDEWEB)

    Rist, D.L.

    1983-01-01

    Interactions involving Botrytis cinerea Pers., B. squamosa Walker, and ozone on onion (alium cepae L.) were investigated. Initially, threshold dosages of ozone required to predispose onion leaves to greater infection by B. cinerea and B. squamosa were determined under controlled conditions in an ozone-exposure chamber. Subsequent experiments supported the hypothesis that nutrients leaking out of ozone-injured cells stimulated lesion production by B. cinerea. The electrical conductivity of, and carbohydrate concentration in, dew collected from leaves of onion plants which had been exposed to ozone were greater than the electrical conductivity of, and carbohydrate concentration in, dew collected from leaves of other, non-exposed onion plants. When conidia of B. cinerea were suspended in dew collected from leaves of plants which had been exposed to ozone and the resulting suspension atomized onto leaves of non-exposed plants, more lesions were induced than on leaves of other non-exposed plants inoculated with conidia suspended in dew collected from plants which had not been exposed to ozone. EDU protected onion leaves from ozone-induced predisposition to these fungi under controlled conditions. Experiments designed to detect interaction between B. cinerea and B. squamosa in onion leaf blighting indicated that such interaction did not occur. Leaves were blighted rapidly when inoculated with B. squamosa whether B. cinerea was present or absent.

  20. Effect of onion flavonoids on colorectal cancer with hyperlipidemia: an in vivo study

    Directory of Open Access Journals (Sweden)

    He Y

    2014-01-01

    Full Text Available Yongshan He,1,* Heiying Jin,1,* Wei Gong,2,* Chunxia Zhang,1 Acheng Zhou1 1National Center of Colorectal Surgery, Third Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing, People's Republic of China; 2Department of Surgery, Jiangyin Hospital of Traditional Chinese Medicine, Jiangyin, People's Republic of China *These authors contributed equally to this work Objectives: This study aims to find the effect of onion's extraction on the colorectal cancer with hyperlipidemia. Method: We established a hyperlipidemia-subcutaneously heterotopic colorectal cancer orthotopic transplant model and fed mice a high fat diet and performing transplantation. Animal models were treated with capecitabine and/or simvastatin and low-, middle-, high- dose of onion's extraction and both tumor growth rate and blood lipid levels were monitored. Results: We found that colorectal cancer in onion's extraction groups was significantly inhibited, and the effect of high dose of onion's extraction was equivalent to capecitabine. Onion's extraction effectively decreased levels of apoB and TC. Conclusion: Our study established a hyperlipidemia colon tumor model involving subcutaneous colon translocation and orthotopic transplantation, this model was an ideal research model for mutual influence of hyperlipidemia and colorectal cancer. Onion's extraction could inhibit the proliferation of colorectal cancer; the function of the high-dose of onion's extraction was fairly to capecitabine, which provided a new direction in protecting and treating colorectal cancer. Keywords: colorectal cancer, hyperlipidemia, onion flavonoids, capecitabine, simvastatin

  1. Onion and weed response to mustard (Sinapis alba) seed meal

    Science.gov (United States)

    Weed control in organic onion production is often difficult and expensive, requiring numerous cultivations and extensive hand-weeding. Onion safety and weed control with mustard seed meal (MSM) derived from Sinapis alba was evaluated in greenhouse and field trials. MSM applied at 110, 220, and 440 g...

  2. Plant growth promotion rhizobacteria in onion production.

    Science.gov (United States)

    Colo, Josip; Hajnal-Jafari, Timea I; Durić, Simonida; Stamenov, Dragana; Hamidović, Saud

    2014-01-01

    The aim of the research was to examine the effect of rhizospheric bacteria Azotobacter chroococcum, Pseudomonas fluorescens (strains 1 and 2) and Bacillus subtilis on the growth and yield of onion and on the microorganisms in the rhizosphere of onion. The ability of microorganisms to produce indole-acetic acid (IAA), siderophores and to solubilize tricalcium phosphate (TCP) was also assessed. The experiment was conducted in field conditions, in chernozem type of soil. Bacillus subtilis was the best producer of IAA, whereas Pseudomonas fluorescens strains were better at producing siderophores and solubilizing phosphates. The longest seedling was observed with the application of Azotobacter chroococcum. The height of the plants sixty days after sowing was greater in all the inoculated variants than in the control. The highest onion yield was observed in Bacillus subtilis and Azotobacter chroococcum variants. The total number of bacteria and the number of Azotobacter chroococcum were larger in all the inoculated variants then in the control. The number of fungi decreased in most of the inoculated variants, whereas the number of actinomycetes decreased or remained the same.

  3. Onion-like microspheres with tricomponent from gelable triblock copolymers.

    Science.gov (United States)

    Zhang, Ke; Gao, Lei; Chen, Yongming; Yang, Zhenzhong

    2010-06-01

    Onion-like functional microspheres with three alternate layers were obtained by aerosol-assisted self-assembly of a functional block copolymer, poly(3-(triethoxysilyl)propyl methacrylate)-block-polystyrene-block-poly(2-vinylpyridine) (PTEPM-b-PS-b-P2VP). Through self-gelation reaction occurred in the PTEPM layers, organic/inorganic hybrid functional spheres with highly ordered concentric curved lamellar structure were prepared. Using these hybrid onion-like microspheres as templates, gold ions were entrapped into the P2VP layers and then gold nanoparticles located in each P2VP layers were formed by a reduction. By dispersing in acidic water, the onion-like polymeric spheres were broken and, as a result, sandwich-like nanoplates with curved morphology were obtained. Copyright © 2010 Elsevier Inc. All rights reserved.

  4. AIB-OR: improving onion routing circuit construction using anonymous identity-based cryptosystems.

    Science.gov (United States)

    Wang, Changji; Shi, Dongyuan; Xu, Xilei

    2015-01-01

    The rapid growth of Internet applications has made communication anonymity an increasingly important or even indispensable security requirement. Onion routing has been employed as an infrastructure for anonymous communication over a public network, which provides anonymous connections that are strongly resistant to both eavesdropping and traffic analysis. However, existing onion routing protocols usually exhibit poor performance due to repeated encryption operations. In this paper, we first present an improved anonymous multi-receiver identity-based encryption (AMRIBE) scheme, and an improved identity-based one-way anonymous key agreement (IBOWAKE) protocol. We then propose an efficient onion routing protocol named AIB-OR that provides provable security and strong anonymity. Our main approach is to use our improved AMRIBE scheme and improved IBOWAKE protocol in onion routing circuit construction. Compared with other onion routing protocols, AIB-OR provides high efficiency, scalability, strong anonymity and fault tolerance. Performance measurements from a prototype implementation show that our proposed AIB-OR can achieve high bandwidths and low latencies when deployed over the Internet.

  5. Adsorption and manipulation of carbon onions on highly oriented pyrolytic graphite studied with atomic force microscopy

    International Nuclear Information System (INIS)

    Zhou Jianfeng; Shen Ziyong; Hou Shimin; Zhao Xingyu; Xue Zengquan; Shi Zujin; Gu Zhennan

    2007-01-01

    Carbon onions produced by DC arc discharge method were deposited on highly oriented pyrolytic graphite (HOPG) surface and their adsorption and manipulation was studied using an atomic force microscopy (AFM). Well-dispersed adsorption of carbon onions on HOPG surface was obtained and aggregations of onions were not observed. The van der Waals interaction between the onion and HOPG surface and that between two onions, were calculated and discussed using Hamaker's theory. The manipulation of adsorbed onions on HOPG surface was realized using the AFM in both the raster mode and the vector mode. The controllability and precision of two manipulation modes were compared and the vector mode manipulation was found superior, and is a useful technique for the construction of nano-scale devices based on carbon onions

  6. Planting time and mulching effect on onion development and seed ...

    African Journals Online (AJOL)

    A field experiment was conducted to evaluate effects of planting time and mulches on bulb growth and seed production of onion (Allium cepa L.) cv. Taherpuri. Planting time and mulches had significant influence on almost all parameters studied. Onion planted on 21 November had better agronomic traits contributing ...

  7. Determination of quercetins in onion (Allium cepa) using infrared spectroscopy.

    Science.gov (United States)

    Lu, Xiaonan; Ross, Carolyn F; Powers, Joseph R; Rasco, Barbara A

    2011-06-22

    The rapid quantification of flavonoid compounds in onions by attenuated total reflectance (ATR) Fourier transform infrared (FT-IR) spectroscopy combined with multivariate analysis was evaluated as a possible alternative to high-performance liquid chromatography (HPLC) analysis. Quercetin content in onion varieties (yellow, red, and sweet) was quantified using ATR FT-IR (4000 to 400 cm⁻¹) spectroscopy and HPLC methods. Quercetin-3,4'-O-diglucoside (3,4'-Qdg) and quercetin-4'-O-glucoside (4'-Qmg) comprised >80% of the total flavonol content detected in the studied varieties. The quercetin compounds (3,4'-Qdg and 4'-Qmg) and total flavonol conjugates were quantified by HPLC, and results correlated closely with ATR-IR values (R > 0.95). Cross-validated (leave-one-out) partial least-squares regression (PLSR) models successfully predicted concentrations of these quercetins. The standard errors of cross-validation (SECV) of 3,4'-Qdg and 4'-Qmg, total quercetin, and total flavonol contents of onions were 20.43, 21.18, and 21.02 mg/kg fresh weight, respectively. In addition, supervised and unsupervised segregation analyses (principal component analysis, discriminant function analysis, and soft independent modeling of class analogue) were performed to classify onion varieties on the basis of unique infrared spectral features. There was a high degree of segregation (interclass distances > 3.0) for the different types of onion. This study indicated that the IR technique could predict 3,4'-Qdg, 4'-Qmg, total quercetin, and total flavonol contents and has advantages over the traditional HPLC method in providing a valid, efficient, and cost-effective method requiring less sample preparation for the quantification of quercetins in onion.

  8. Change of quality characteristics of onion during merchandising in retail trade

    Directory of Open Access Journals (Sweden)

    V. V. Andreev

    2018-01-01

    Full Text Available When selling fruits and vegetables in retail stores, the question arises of the preservation of their consumer properties. Therefore, the task was set to follow the changes in the quality of onions in a trading hall for seven days, which corresponds to the time of sale of the goods. Onions, unlike other vegetables, less interact with the air. Therefore, the loss of moisture and organic substances is insignificant. The paper presents data on the change in the qualitative characteristics of onions in the conditions of a trading hall and refrigerated storages for 7 days. Obtaining and processing of experimental data on the changes in sugar onion, ascorbic acid and moisture content was carried out in the laboratories of commodity science and food products expertise of the Department of Trade and Goods Science of the St. Petersburg State University of Economics. The partial increase in acidity during prolonged storage could be a consequence of the breakdown of sugars during fermentation. As a result of the conducted researches it is established that the biochemical processes taking place in onions practically do not depend on the temperature decrease in the room. It is recommended to pre-dry the onions for better preservation. It is necessary to carry out quality control of the product by organoleptic and physicochemical parameters during refrigerated storage and storage in the trading floor. It is established that the content of vitamin C does not decrease in the process of realization of onion if the humidity of air does not exceed 65%. The experiments showed that the loss of sugars during storage does not occur. Essential loss of moisture, and consequently, a decrease in the mass of the product, was not observed.

  9. Consumer opinions in Argentina on food irradiation: irradiated onions

    International Nuclear Information System (INIS)

    Curzio, O.A.; Croci, C.A.

    1998-01-01

    Two surveys were carried out in Buenos Aires of consumer attitudes towards irradiated onions [no data given]. The first investigated the general level of consumer knowledge concerning food irradiation, whilst the second (which covered consumers who had actually bought irradiated onions) examined reasons for purchase and consumer satisfaction. Results reveal that more than 90% of consumers surveyed had a very limited knowledge of food irradiation

  10. 78 FR 56816 - Vidalia Onions Grown in Georgia; Change in Reporting and Assessment Requirements

    Science.gov (United States)

    2013-09-16

    ... to regulation under the order and approximately 80 onion producers in the designated production area...; FV13-955-1 FIR] Vidalia Onions Grown in Georgia; Change in Reporting and Assessment Requirements AGENCY... the reporting and assessment requirements prescribed under the marketing order for Vidalia onions...

  11. Changes in endogenous growth inhibitors in onion bulbs (Allium cepa L. cv. Sochaczewska during storage

    Directory of Open Access Journals (Sweden)

    Elżbieta Kielak

    2013-12-01

    Full Text Available Changes in inhibitor activity in the onion bulbs (Allium cepa L. cv. Sochaczewska during storage were investigated. Onions were dried under an umbrella roof until October 15th or November 15th and thereafter stored in a cold-room at 0-1°C until May 15th. The activity of inhibitors fluctuated markedly during the storage period. At least two peaks and two decreases of inhibitor activity were observed. The weather conditions seemed to strongly influence the level and the date of appearance of inhibitors in onions. Higher inhibitor activity is usually connected with better storage and less sprouting of onions during storage. Prolonged drying under an umbrella roof enhanced onion quality after storage only in these cases when it actually improved the drying of onions.

  12. Application of forwardchaining method to diagnosis of onion plant diseases

    Science.gov (United States)

    Sitanggang, Delima; Siregar, Saut D.; Situmeang, Suryani M. F.; Indra, Evta; Sagala, Ayu R.; Sihombing, Oloan; Nababan, Marlince; Pasaribu, Hendra; Damanik, Rudolf R.; Turnip, Mardi; Saragih, Rijois I. E.

    2018-04-01

    Red Onion is a tuber plant that is widely used by the people of Indonesia, both as herbs and herbal medicines. Onion farmers have limitations in identifying diseases that attack their crops.This disease can cause crop failure against the onion.This design begins with the creation of a knowledge base up to input-output design with forward chaining method. The results of this design can assist farmers in identifying their plant diseases. Based on diagnostic results of several methods that have been done testing can diagnose diseases contained in onion plants. With symptoms data that has been determined by the expert with the value of each symptom is different. As for the symptoms that have been determined that the leaves contain patches with a value of 0.3, White leaf spots value 0.4, Leaf spots form a purple zone if it is severe 0.5, Leaf tip of 0.2, Tubers rot 0.4. Based on the above diagnostic results then get the value of diagnosis 67% forward chaining with trotol disease type, Purple spotting.

  13. Strip Tillage and Early-Season Broadleaf Weed Control in Seeded Onion (Allium cepa

    Directory of Open Access Journals (Sweden)

    Sarah Gegner-Kazmierczak

    2016-03-01

    Full Text Available Field experiments were conducted in 2007 and 2008 near Oakes, North Dakota (ND, USA, to evaluate if strip tillage could be incorporated into a production system of seeded onion (Allium cepa to eliminate the standard use of a barley (Hordeum vulgare companion crop with conventional, full width tillage, yet support common early-season weed control programs. A split-factor design was used with tillage (conventional and strip tillage as the main plot and herbicide treatments (bromoxynil, DCPA, oxyfluorfen, and pendimethalin as sub-plots. Neither tillage nor herbicide treatments affected onion stand counts. Common lambsquarters (Chenopodium album densities were lower in strip tillage compared to conventional tillage up to three weeks after the post-emergence applied herbicides. In general, micro-rate post-emergence herbicide treatments provided greater early-season broadleaf weed control than pre-emergence herbicide treatments. Onion yield and grade did not differ among herbicide treatments because the mid-season herbicide application provided sufficient control/suppression of the early-season weed escapes that these initial weed escapes did not impact onion yield or bulb diameter. In 2007, onion in the strip tillage treatment were larger in diameter resulting in greater total and marketable yields compared to conventional tillage. Marketable onion yield was 82.1 Mg ha−1 in strip tillage and 64.9 Mg ha−1 in conventional tillage. Results indicate that strip tillage use in direct-seeded onion production was beneficial, especially when growing conditions were conducive to higher yields and that the use of strip tillage in onion may provide an alternative to using a companion crop as it did not interfere with either early-season weed management system.

  14. Valorization of onion waste and by-products: MCR-ALS applied to reveal the compositional profiles of alcoholic fermentations of onion juice monitored by near-infrared spectroscopy.

    Science.gov (United States)

    González-Sáiz, José-María; Esteban-Díez, Isabel; Rodríguez-Tecedor, Sofía; Pizarro, Consuelo

    2008-11-01

    The overall purpose of the project, of which this study is a part, was to examine the feasibility of onion waste as a support-substrate for the profitable production of food-grade products. This study focused on the efficient production of ethanol from worthless onions by transforming the onion juice into onion liquor via alcoholic fermentation with the yeast Saccharomyces cerevisiae. The onion bioethanol produced could be later used as a favorable substrate for acetic fermentation to finally obtain onion vinegar. Near-infrared spectroscopy (NIRS), coupled with the multivariate curve resolution-alternating least squares (MCR-ALS) method, has been used to reveal the compositional and spectral profiles for both substrates and products of alcoholic fermentation runs, that is, total sugars, ethanol, and biomass concentration. The ambiguity associated with the ALS calculation was resolved by applying suitable inequality and equality constraints. The quality of the results provided by the NIR-based MCR-ALS methodology adopted was evaluated by several performance indicators, including the variance explained by the model, the lack of fit and the agreement between the MCR-ALS achieved solution and the results computed by applying previously validated PLS reference models. An additional fermentation run was employed to test the actual predictive ability of the ALS model developed. For all the components resolved in the fermentation system studied (i.e., total sugars, ethanol, and biomass), the final model obtained showed a high predictive ability and suitable accuracy and precision, both in calibration and external validation, confirmed by the very good agreement between the ALS responses and the reference values (the coefficient of determination was, in all cases, very close to 1, and the statistics confirmed that no significant difference was found between PLS reference models and the MCR-ALS methodology applied). Thus, the proven reliability of the MCR-ALS model presented

  15. Method to obtain carbon nano-onions by pyrolisys of propane

    Science.gov (United States)

    Garcia-Martin, Tomas; Rincon-Arevalo, Pedro; Campos-Martin, Gemma

    2013-11-01

    We present a new and simple method for carbon nano-onions (CNOs) production which is based on the pyrolysis of Propane. CNOs are originated in a laminar premixed Propane/Oxygen flame of approximately 1.8 of stoichiometric coefficient. The stream of gasses resulting from the combustion drives the carbon particles towards the aluminium surface on which nano-onions are deposited and collected. The structure and size of the deposited carbon onion on the metal wall are characterized by High Resolution Transmission Electron Microscopy technique (HRTEM). The experimental images show the presence of two different types of CNOs. The first particles have diameters in the range of 18-25 nm and the second ones around 10 nm.

  16. Fresh onion juice enhanced copulatory behavior in male rats with and without paroxetine-induced sexual dysfunction.

    Science.gov (United States)

    Allouh, Mohammed Z; Daradka, Haytham M; Al Barbarawi, Mohammed M; Mustafa, Ayman G

    2014-02-01

    Onion (Allium cepa) is one of the most commonly cultivated species of the family Liliaceae, and has long been used in dietary and therapeutic applications. Treatment with fresh onion juice has been reported to promote testosterone production in male rats. Testosterone is the male sex hormone responsible for enhancing sexual libido and potency. This study aimed to investigate the effects of onion juice on copulatory behavior of sexually potent male rats and in male rats with paroxetine-induced sexual dysfunction. Sexually experienced male rats were divided into seven groups: a control group, three onion juice-treated groups, a paroxetine-treated group, and two groups treated with paroxetine plus different doses of onion juice. At the end of the treatments, sexual behavior parameters and testosterone levels were measured and compared among the groups. Administration of onion juice significantly reduced mount frequency and latency and increased the copulatory efficacy of potent male rats. In addition, administration of onion juice attenuated the prolonged ejaculatory latency period induced by paroxetine and increased the percentage of ejaculating rats. Serum testosterone levels increased significantly by onion juice administration. However, a significant reduction in testosterone because of paroxetine therapy was observed. This reduction was restored to normal levels by administration of onion juice. This study conclusively demonstrates that fresh onion juice improves copulatory behavior in sexually potent male rats and in those with paroxetine-induced sexual dysfunction by increasing serum testosterone levels.

  17. Effect of medium and post-irradiation storage on rooting of irradiated onions

    International Nuclear Information System (INIS)

    Singh, Rita

    2000-01-01

    Rooting test for detection of irradiation in onion bulbs was studied. Onions were exposed to different dose levels of 30, 60, 90, 120 and 150 Gy. The effects of irradiation dose, cultivar difference, rooting medium and post-irradiation storage on the rooting were investigated. The number and the length of the roots formed in onions were found to decrease on irradiation. The effect was more at higher doses. The effect of irradiation on rooting was also evident after 120 days of storage. (author)

  18. Anaerobic digestion of onion residuals using a mesophilic Anaerobic Phased Solids Digester

    International Nuclear Information System (INIS)

    Romano, Rowena T.; Zhang, Ruihong

    2011-01-01

    The anaerobic digestion of onion residual from an onion processing plant was studied under batch-fed and continuously-fed mesophilic (35 ± 2 o C) conditions in an Anaerobic Phased Solids (APS) Digester. The batch digestion tests were performed at an initial loading of 2.8 gVS L -1 and retention time of 14 days. The biogas and methane yields, and volatile solids reduction from the onion residual were determined to be 0.69 ± 0.06 L gVS -1 , 0.38 ± 0.05 L CH 4 gVS -1 , and 64 ± 17%, respectively. Continuous digestion tests were carried out at organic loading rates (OLRs) of 0.5-2.0 gVS L -1 d -1 . Hydrated lime (Ca(OH) 2 ) was added to the APS-Digester along with the onion residual at 16 mg Ca(OH) 2 gVS -1 to control the pH of the biogasification reactor above 7.0. At steady state the average biogas yields were 0.51, 0.56, and 0.62 L gVS -1 for the OLRs of 0.5, 1.0, and 2.0 gVS L -1 d -1 respectively. The methane yields at steady state were 0.29, 0.32, and 0.31 L CH 4 gVS -1 for the OLRs of 0.5, 1.0, and 2.0 gVS L -1 d -1 respectively. The study shows that the digestion of onion residual required proper alkalinity and pH control, which was possible through the use of caustic chemicals. However, such chemicals will begin to have an inhibitory effect on the microbial population at high loading rates, and therefore alternative operational parameters are needed. -- Highlights: → An APS-Digester was used to study biogas production from onion solid residues. → Biogas and methane yields from onion solids were determined. → Study showed substantial findings for treating onion solid residues.

  19. Companion cropping with potato onion enhances the disease resistance of tomato against Verticillium dahliae

    Directory of Open Access Journals (Sweden)

    Xuepeng eFu

    2015-09-01

    Full Text Available Intercropping could alleviate soil-borne diseases, however, few studies focused on the immunity of the host plant induced by the interspecific interactions. To test whether or not intercropping could enhance the disease resistance of host plant, we investigated the effect of companion cropping with potato onion on tomato Verticillium wilt caused by Verticillium dahliae (V. dahliae. To investigate the mechanisms, the root exudates were collected from tomato and potato onion which were grown together or separately, and were used to examine the antifungal activities against V. dahliae in vitro, respectively. Furthermore, RNA-seq was used to examine the expression pattern of genes related to disease resistance in tomato companied with potato onion compared to that in tomato grown alone, under the condition of infection with V. dahliae. The results showed that companion cropping with potato onion could alleviate the incidence and severity of tomato Verticillium wilt. The further studies revealed that the root exudates from tomato companied with potato onion significantly inhibited the mycelia growth and spore germination of V. dahliae. However, there were no significant effects on these two measurements for the root exudates from potato onion grown alone or from potato onion grown with tomato. RNA-seq data analysis showed the disease defense genes associated with pathogenesis-related proteins, biosynthesis of lignin, hormone metabolism and signal transduction were expressed much higher in the tomato companied with potato onion than those in the tomato grown alone, which indicated that these defense genes play important roles in tomato against V. dahliae infection, and meant that the disease resistance of tomato against V. dahliae was enhanced in the companion copping with potato onion. We proposed that companion cropping with potato onion could enhance the disease resistance of tomato against V. dahliae by regulating the expression of genes related

  20. Association of Pectolytic Fluorescent PSeudomonas with Postharvest Rots of Onion

    Directory of Open Access Journals (Sweden)

    H.H. El-Hendawy

    2004-12-01

    Full Text Available Five isolates of pectolytic fluorescent pseudomonads were obtained from a rotted onion bulb and identified as Pseudomonas marginalis. At both 4 and 25oC, all isolates caused soft rot to detached plant parts of onion and to carrot, celery, cucumber, pepper, spinach, tomato and turnip (but not garlic. They did not however cause any symptoms in living plants of these same species. These results suggest that the onion isolates are a postharvest pathogen which is not destructive in the field but becomes a threat to fresh vegetables stored at low-temperature. Analysis of cellulosolytic and pectic enzymes revealed that pectic lyases, but not polygalacturonases, pectin methyl esterases and cellulases were produced in culture by each isolate.

  1. 76 FR 31575 - United States Standards for Grades of Frozen Onions

    Science.gov (United States)

    2011-06-01

    ... processors of frozen onions in the United States. The petition provided information on style, sample size... change in the style designations for minced style, and a correction to the text. The members agreed with the proposed section concerning requirements for Styles, Type I, Whole onions and Type II, Pearl...

  2. 78 FR 8047 - Onions Grown in South Texas; Increased Assessment Rate

    Science.gov (United States)

    2013-02-05

    ... Executive Order 12988, Civil Justice Reform. Under the marketing order now in effect, South Texas onion... DEPARTMENT OF AGRICULTURE Agricultural Marketing Service 7 CFR Part 959 [Doc. No. AMS-FV-12-0039; FV12-959-1 PR] Onions Grown in South Texas; Increased Assessment Rate AGENCY: Agricultural Marketing...

  3. Increase of onion yield through low dose of gamma irradiation of its seeds

    International Nuclear Information System (INIS)

    Wiendl, F.M.; Wiendl, F.W.; Wiendl, J.A.; Vedovatto, A.; Arthur, V.

    1995-01-01

    The increase of onions' yield could be achieved by the common farmer through the use of nuclear techniques. This report describes the results obtained with the irradiation of onion seeds, with low doses of gamma radiations (Cobalt-60), at doses of 0 (control), 150, 400 and 700 Gy. Beyond the proper onion's variety als use of low dose rates of 13.1, 39.2 and 52.3 Gy per hour were of the great importance during irradiation. The results showed to be promising both in laboratory studies and in the field, resulting in an increase of onions production: A greater number of seedlings, bulbs and a higher yield in weight per hectar were planted. In the field the most promising dose and dose rate to the variety ''Super-X'' were respectively 150 Gy and 13.1 Gy per hour, yielding an 24.9 percent heavier weight of onions than the control. The other tested variety was ''Granex-33'', which did not respond so favorable to irradiation. However, also with this variety we harvested a 2.1 percent heavier weight than its control, if the onion seeds were irradiated with the dose of 700 Gy at a dose rate of 13.1 Gy per hour. (Author)

  4. Effects of an onion by-product on bioactivity and safety markers in healthy rats

    DEFF Research Database (Denmark)

    Roldan-Marin, Eduvigis; Krath, Britta; Poulsen, Morten

    2009-01-01

    Onions are excellent sources of bioactive compounds including fructo-oligosaccharides (FOS) and polyphenols. An onion by-product was characterised in order to be developed as a potentially bioactive food ingredient. Our main aim was to investigate whether the potential health and safety effects...... of this onion by-product were shared by either of two derived fractions, an extract containing the onion FOS and polyphenols and a residue fraction containing mainly cell wall materials. We report here on the effects of feeding these products on markers of potential toxicity, protective enzymes and gut...... environment in healthy rats. Rats were fed during 4 weeks with a diet containing the products or a control feed balanced in carbohydrate. The onion by-product and the extract caused anaemia as expected in rodents for Allium products. No other toxicity was observed, including genotoxicity. Glutathione...

  5. Variation of quercetin glycoside derivatives in three onion (Allium cepa L. varieties

    Directory of Open Access Journals (Sweden)

    Jung-Ho Kwak

    2017-09-01

    Full Text Available The aim of this study was to quantify the contents of individual quercetin glycosides in red, yellow and chartreuse onion by High Performance Liquid Chromatography (HPLC analysis. Acid hydrolysis of individual quercetin glycosides using 6 M hydrochloric acid guided to identify and separate quercetin 7,4′-diglucoside, quercetin 3-glucoside, quercetin 4′-glucoside, and quercetin. The contents of total quercetin glycosides varied extensively among three varieties (ranged from 16.10 to 103.93 mg/g DW. Quercetin was the predominant compound that accounted mean 32.21 mg/g DW in red onion (43.6% of the total and 127.92 mg/g DW in chartreuse onion (78.3% of the total followed by quercetin 3-glucoside (28.83 and 24.16 mg/g DW respectively. Quercetin 3-glucoside levels were much higher in yellow onion (43.85 mg/g DW followed by quercetin 30.08 mg/g DW. Quercetin 4′-glucoside documented the lowest amount that documented mean 2.4% of the total glycosides. The varied contents of glycosides present in the different onion varieties were significant.

  6. STRUCTURE OF THE FRESH ONION MARKET IN THE SPRING SEASON: A FOCUS ON TEXAS AND ITS COMPETITION

    OpenAIRE

    Fuller, Stephen W.; Capps, Oral, Jr.; Bello, Haruna; Shafer, Carl E.

    1991-01-01

    A structural model of the spring onion economy is developed to analyze forces affecting the onion-producing sector in Texas. Spring onion prices in Texas are influenced by own shipments, shipments from storage stocks, and variety. Texas's decline in market share is largely the result of expanded late summer (storage) onion production. A decline in the U.S. real tariff and a weakening of the real exchange rate (pesos/$) encouraged onion imports from Mexico during the study period. However, thi...

  7. Effect of Planting Methods on Maturity and Yield of Onion (Allium ...

    African Journals Online (AJOL)

    Key words: onion, planting method, sets, transplantings, direct seeding, bulb yield. .... The central four rows of each plot were harvested and ... Means with lower case letters represent values for planting methods effects and means with upper .... Acta Hort. 84: 27-32. Brewster, J.L. 2002. Onions and other vegetable Alliums.

  8. Effect of Plant Growth Regulator on Red Onion Cultivation from True Seed Shallot (TSS

    Directory of Open Access Journals (Sweden)

    Tri Sudaryono

    2018-01-01

    Full Text Available Red onion is one of the strategic horticultural commodities, considering this commodity is very high consumption as a daily spice and fluctuating price. Therefore is not surprising that these commodities are contributing to inflation. Efforts to meet increasing consumption needs, it is necessary to find the right strategy to increase domestic red onion production. One of the strategies considered to increase domestic red onion production is the use of botanical seed (TSS as a source of seed on shallot cultivation. There are 2 main weaknesses of red onion cultivation with TSS as a source of seeds. The two weaknesses are TSS low growing power, which is naturally only in the 50-60 % range and the number of tubers produced is less than 3 cloves per plant. In order to solve the problem, research has been done to know the effect of plant growth regulator on the growth and red onion production from TSS and also get the description of red onion farming from TSS and tuber as seed source. The research was conducted from June to November 2017 at BPP Pare, Kediri Regency, East Java. The results showed that the use of young coconut water on TSS obtained red onion plants are able to produce the number of tubers per plant more than 3 cloves. In detail as much as 22.22 % produces the number of tubers range 4-5 per plant; 56.56 % yields 5-6 bulb range; and as much as 22.22 % produces tubers > 6. As well, wet weight of tubers when harvested weighing more than 99 g per plant. If converted per hectare, TSS red onion plants treated with young coconut water can produce a range of 30 -35 tons of wet bulb. This production is doubled compared to the production of shallots grown from tubers. Based on the analysis of the farm, red onion from TSS treated with young coconut water gives a profit of Rp 224,860,000 per hectare with B/C ratio of 3.397. This profit is more than 1.75 times compared to the profit of red onion tuber farming which is only Rp 93.787.000, - with B

  9. Effect of planting methods on maturity and yield of onion ( Allium ...

    African Journals Online (AJOL)

    Onion dry bulb are commonly established in the field either by direct sowing of seeds to the field, or by transplanting seedling from seedbed or from sets depending on the growing conditions of the specific region. However, the potential of the different methods have not been tested particularly in the potential onion ...

  10. Gamma, electron beam and ultraviolet radiation on control of storage rots and quality of Walla Walla onions

    International Nuclear Information System (INIS)

    Lu, J.Y.; Stevens, C.; Yakubu, P.; Loretan, P.A.; Eakin, D.

    1988-01-01

    Walla Walla onions were irradiated with doses of 0.1, 0.3, 1.0, 2.0 and 3.0 kGy of gamma rays; 0.1, 1.0, 2.0, 3.0 and 5.0 kGy of electron beams; or 0.44 × 10 4 1.32 × 10 4 , 3.52 × 10 4 , 7.33 × 10 4 and 19.1 × 10 4 erg/mm± of UV. The onions were stored up to four weeks at 20–25 C. UV irradiated onions exhibited the greatest percentage of marketable onions and reduction in postharvest rots. Sprouting was obsened with control, UV and electron beam irradiated onions but not with gamma irradiated onions. Effect of gamma, electron beams and UV on pH, moisture, ascorbic acid and color were not significant. Onions became soft with the high dose of gamma radiation (3.0 kGy). Total sugar content was not affected by UV and electron beam but tended to be greatest at the 1.0 kGy gamma radiation. The effect of the radiation on the onion sensory scores was not clearly indicated except that 3.0 kGy gamma ray irradiated onions had the lowest firmness score

  11. Enhancing shelf life of minimally processed multiplier onion using silicone membrane.

    Science.gov (United States)

    Naik, Ravindra; Ambrose, Dawn C P; Raghavan, G S Vijaya; Annamalai, S J K

    2014-12-01

    The aim of storage of minimal processed product is to increase the shelf life and thereby extend the period of availability of minimally processed produce. The silicone membrane makes use of the ability of polymer to permit selective passage of gases at different rates according to their physical and chemical properties. Here, the product stored maintains its own atmosphere by the combined effects of respiration process of the commodity and the diffusion rate through the membrane. A study was undertaken to enhance the shelf life of minimally processed multiplier onion with silicone membrane. The respiration activity was recorded at a temperature of 30 ± 2 °C (RH = 60 %) and 5 ± 1 °C (RH = 90 %). The respiration was found to be 23.4, 15.6, 10 mg CO2kg(-1)h(-1) at 5 ± 1 °C and 140, 110, 60 mg CO2kg(-1) h(-1) at 30 ± 2° for the peeled, sliced and diced multiplier onion, respectively. The respiration rate for the fresh multiplier onion was recorded to be 5, 10 mg CO2kg(-1) h(-1) at 5 ± 1 °C and 30 ± 1 ° C, respectively. Based on the shelf life studies and on the sensory evaluation, it was found that only the peeled multiplier onion could be stored. The sliced and diced multiplier onion did not have the required shelf life. The shelf life of the multiplier onion in the peel form could be increased from 4-5 days to 14 days by using the combined effect of silicone membrane (6 cm(2)/kg) and low temperature (5 ± 1 °C).

  12. Multi-scale structure and topological anomaly detection via a new network statistic: The onion decomposition.

    Science.gov (United States)

    Hébert-Dufresne, Laurent; Grochow, Joshua A; Allard, Antoine

    2016-08-18

    We introduce a network statistic that measures structural properties at the micro-, meso-, and macroscopic scales, while still being easy to compute and interpretable at a glance. Our statistic, the onion spectrum, is based on the onion decomposition, which refines the k-core decomposition, a standard network fingerprinting method. The onion spectrum is exactly as easy to compute as the k-cores: It is based on the stages at which each vertex gets removed from a graph in the standard algorithm for computing the k-cores. Yet, the onion spectrum reveals much more information about a network, and at multiple scales; for example, it can be used to quantify node heterogeneity, degree correlations, centrality, and tree- or lattice-likeness. Furthermore, unlike the k-core decomposition, the combined degree-onion spectrum immediately gives a clear local picture of the network around each node which allows the detection of interesting subgraphs whose topological structure differs from the global network organization. This local description can also be leveraged to easily generate samples from the ensemble of networks with a given joint degree-onion distribution. We demonstrate the utility of the onion spectrum for understanding both static and dynamic properties on several standard graph models and on many real-world networks.

  13. Economical feasibility of irradiation method for the preservation of onions and potatoes in Libya

    International Nuclear Information System (INIS)

    Sharif, M.M.

    1990-01-01

    Gamma irradiation of onions and potatoes in order to increase their storage life, offers an alternative method to the conventional cold-storage (refrigeration) method. In this paper we discuss economical feasibility of this gamma irradiation method for commercial use. Gamma radiation emitted from source Co-60 (wet type 651) was used to irradiate onions and potatoes. Simultaneously, a cold-storage study was conducted using refrigeration (Fiocchetti Luzzary, CBE, Italy). The efficiency of the two methods, in the preservation of onions and potatoes for a storage period of 8 months, was compared by estimating the cost incurred for irradiation and refrigeration. The preservation cost by the irradiation method is estimated at 0.550 and 0.520 Libyan Dinars (1 L.D. = 3.30 US $) per Kg material for potatoes and onions respectively. In comparison with these costs, the refrigeration method works out at a higher cost of 0.730 and 0.960 Libyan Dinars per Kg material for potatoes and onions respectively. (author)

  14. Correlations between colonization of onion thrips and leaf reflectance measures across six cabbage varieties.

    Directory of Open Access Journals (Sweden)

    János Bálint

    Full Text Available The main purpose of this study was to reveal if the UV-A, and visible light reflection of leaves of white cabbage varieties is correlated to resistance against onion thrips. The antixenotic resistance (AR against onion thrips and thrips damage differed between varieties Balashi, Bloktor, Riana - considered resistant - and Green Gem, Hurricane, Quisor - considered susceptible. The solar UV-A (340-400 nm and visible (401-650 nm light reflection of white cabbage leaves were recorded. Correlation between AR against onion thrips and reflection of leaves in UV-A and visible range of the studied white cabbage varieties were computed. According to the AR evaluation onion thrips density was always higher on susceptible than on resistant varieties. The UV-A light reflection of head forming leaves and the contrast between head and exterior leaves (H/E was negatively correlated with onion thrips host preference at an early stage of cabbage head formation. The visible light reflection of both head forming and exterior leaves was also negatively correlated with onion thrips host preference. Susceptible varieties had greater damage ratings at harvest than resistant ones and positive correlations were observed between AR and damage. AR against onion thrips may be affected by differences in reflection of cabbage leaves at an early growth stage. It is suggested that more intensive reflection of leaves and/or higher contrast values between the reflectance intensity of head versus outer leaves made the resistant varieties less attractive to onion thrips. Our results reported here provide the first evidence of negative correlation between UV-A and visible reflection of leaves and AR of white cabbage against a dangerous insect pest, opening new perspectives for understanding the role of reflection by plant leaves in pest management.

  15. Effectiveness of Onion Extract Gel on Surgical Scars in Asians

    Directory of Open Access Journals (Sweden)

    Kumutnart Chanprapaph

    2012-01-01

    Full Text Available Background. Onion extracts have been shown in vitro to accelerate wound healing. Results from clinical studies on surgical scars in Caucasians were disappointing. The aim of this study is to evaluate the effectiveness of onion extract gel in improving the cosmetic and symptoms of surgical scars in Asians. Patients/Methods. Twenty Asians who had new Pfannenstiel’s cesarean section scars were recruited in this prospective double-blinded, split-scar study. Each side was randomly assigned treatment with onion extract gel or placebo at 7 days after surgery. The product was applied three times daily for 12 weeks. Subjects were evaluated at baseline and 4th and 12th weeks. Scar redness was assessed by calorimeter, scar height and pliability were assessed by blinded investigators, and scar symptoms and overall cosmetic improvement were assessed by subjects. Results. Sixteen subjects completed the study. A statistically significant difference between two sides of scar in terms of scar height and scar symptoms was found. There was no statistically significant difference in scar redness, scar pliability, and overall cosmetic appearance between two sides. Conclusions. The early use of topical 12% onion extract gel on Pfannenstiel’s cesarean section scar in Asians resulted in the improvement of scar height and scar symptoms.

  16. Ozone and Botrytis interactions in onion-leaf dieback: open-top chamber studies

    Energy Technology Data Exchange (ETDEWEB)

    Wukasch, R.T.; Hofstra, G.

    1977-09-01

    Paired open-top chambers were used to study interactions between Botrytis spp. and ozone in field-grown onions. Charcoal filters removed 35 to 65% of the ambient ozone, resulting in six-fold reduction of onion leaf dieback and a 28% increase in onion yield compared with unfiltered chambers. Symptoms of leaf injury appeared soon after ozone levels exceeded 294 ..mu..g/m/sup 3/ (0.15 ppm) for 4 hr. Lesions caused by Botrytis were few because no dew formed in the chambers. However, when leaves were wetted with foggers, inoculation with mycelial suspensions of B. sauamosa in late August produced significantly more lesions and leaf dieback in the unfiltered chamber. Botrytis squamosa, B. cinerea, B. allii, and several genera of secondary fungi were isolated from these lesions. Botrytis squamosa was recovered from lesions only, whereas B. cinerea and B. allii were associated more generally with onion leaf tissue regardless of lesions. 25 references, 1 figure, 2 tables.

  17. Growth limit of carbon onions – A continuum mechanical study

    DEFF Research Database (Denmark)

    Todt, Melanie; Bitsche, Robert; Hartmann, Markus A.

    2014-01-01

    of carbon onions and, thus, can be a reason for the limited size of such particles. The loss of stability is mainly evoked by van der Waals interactions between misfitting neighboring layers leading to self-equilibrating stress states in the layers due to mutual accommodation. The influence of the curvature......The growth of carbon onions is simulated using continuum mechanical shell models. With this models it is shown that, if a carbon onion has grown to a critical size, the formation of an additional layer leads to the occurrence of a structural instability. This instability inhibits further growth...... induced surface energy and its consequential stress state is investigated and found to be rather negligible. Furthermore, it is shown that the nonlinear character of the van der Waals interactions has to be considered to obtain maximum layer numbers comparable to experimental observations. The proposed...

  18. "Peeling an onion"

    DEFF Research Database (Denmark)

    Kristensen, Liv Kondrup

    2018-01-01

    This article proposes a methodological framework for analyzing video by adopting an embodied perspective. In order to deal with researching the complexity of human interaction that has been captured on video, structured ways for analysis are needed. In this article, the metaphor of an onion is used...... through this methodological approach, a worked example of video observations featuring classroom interaction is presented. While analysis of video through the step-by-step process in four layers is laborious, it is forcing the researcher to break with the habit of privileging talk as the base...

  19. Radioinhibition process in Argentinian garlic and onion bulbs

    Science.gov (United States)

    Curzio, O. A.; Croci, C. A.

    Technological aspects of garlic and onion bulbs subjected to the radioinhibition process and extended storage under warehouse conditions were studied. Garlic and onion of the "Colorado" and "Valenciana sintética 14" varieties respectively, were irradiated in dormancy period with an average dose of 50.0 Gy of 60Co gamma rays and kept in storage up to ten months post-harvest. Throughout the control period (180-300 days post-harvest) obvious benefits were attained as to reducing the weight loss and increasing the percentage of marketable bulbs. In general, the irradiated bulbs were superior to the non-irradiated ones with regard to the external aspect, firmness and internal aspect, while the odor of the bulbs was not affected by the process. The radioinhibition process does not seem to affect adversely the levels of dry matter, carbohydrates and ascorbic acid as well as the acidity in onion bulbs. In two marketing trials a very favourable reception was perceived in the consumer public regarding the quality of the products. These studies have promoted the construction of a multipurpose irradiation facility in the Universidad Nacional del Sur for the development of the radiation processing technology.

  20. Onions in the farming systems of the Swat Valley, Northern Pakistan : Implications for research and extension

    Directory of Open Access Journals (Sweden)

    Defoer, T.

    1993-01-01

    Full Text Available Onion cultivation in Swat Valley, Northern Pakistan, is increasingly becoming important, replacing wheat in the Rabi (winter season. The area increased from 1000 ha in 1986 to 3000 ha in 1991. Due to its relatively recent importance as a cash crop, information on : (1 management practices, (2 factors determining yields, and (3 the economics of onion cultivation, was hardly available. A diagnostic study organized in 1991 addresses these issues. Its results indicate that onion yields and the economics of onion cultivation are significantly affected by : (1 high seed rates in nurseries, (2 use of poorly drained basin type nurseries, (3 late transplanting, (4 high weed infestation in onion fields, and (5 early harvesting. Despite the 67 % higher input costs, induced by seed rates 20 times higher as usually recommended, onion cultivation in Swat Valley is attractive. Net benefits amount to approximately Rs. 45000 (1800 US$ per ha or 2 times as high as the net benefits per ha of wheat cultivation.

  1. Field Evidence Supporting Conventional Onion Curing Practices as a Strategy To Mitigate Escherichia coli Contamination from Irrigation Water.

    Science.gov (United States)

    Wright, Daniel; Feibert, Erik; Reitz, Stuart; Shock, Clint; Waite-Cusic, Joy

    2018-03-01

    The Produce Safety Rule of the U.S. Food Safety Modernization Act includes restrictions on the use of agricultural water of poor microbiological quality. Mitigation options for poor water quality include the application of an irrigation-to-harvest interval of onion production includes an extended irrigation-to-harvest interval (onion fields (randomized block design; n = 5) via drip tape on the final day of irrigation. Onions remained undisturbed for 7 days and were then lifted to the surface to cure for an additional 21 days before harvest. Water, onions, and soil were tested for presence of rifampin-resistant E. coli. One day after irrigation, 13.3% of onions (20 of 150) receiving the poorest quality water (3 log CFU/mL) tested positive for E. coli; this prevalence was reduced to 4% (6 of 150 onions) after 7 days. Regardless of inoculum level, E. coli was not detected on any onions beyond 15 days postirrigation. These results support conventional dry bulb onion curing practices as an effective strategy to mitigate microbiological concerns associated with poor quality irrigation water.

  2. Understanding youth motivation for water onion (Crinum thaianum J. Schulze conservation in Thailand

    Directory of Open Access Journals (Sweden)

    Nuttasun Athihirunwong

    2018-01-01

    Full Text Available Water Onion is an aquatic plant endemic to the coastal plains of southern Thailand. The species is listed as endangered on the IUCN Red List. Despite rapidly declining stocks, the species is not protected under any Thai legislation nor under the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES. At the local level, Water Onion is protected and conserved by young people and adults for various socio-economic reasons. The study explored the participation and underlying conservation motivations of 312 youths in Kapoe district, Ranong province. Using principal component analysis, the youth's motivation for Water Onion conservation was classified into four categories: pro-social, pro-nature, social image, and extrinsic. The results from a logit regression indicated that pro-nature is one of the key motivational factors enhancing actual youth participation in the protection and conservation of Water Onion. It is important for policy makers to understand the effects of various types of motivation on different policy mechanisms in order to craft more effective policies that can further enhance youth participation in conservation initiatives. Keywords: biodiversity conservation, endemic species, pro-nature motivation, water onion, youth

  3. Consequences of co-applying insecticides and fungicides for managing Thrips tabaci (Thysanoptera: Thripidae) on onion.

    Science.gov (United States)

    Nault, Brian A; Hsu, Cynthia L; Hoepting, Christine A

    2013-07-01

    Insecticides and fungicides are commonly co-applied in a tank mix to protect onions from onion thrips, Thrips tabaci Lindeman, and foliar pathogens. Co-applications reduce production costs, but past research shows that an insecticide's performance can be reduced when co-applied with a fungicide. An evaluation was made of the effects of co-applying spinetoram, abamectin and spirotetramat with commonly used fungicides, with and without the addition of a penetrating surfactant, on onion thrips control in onion fields. Co-applications of insecticides with chlorothalonil fungicides reduced thrips control by 25-48% compared with control levels provided by the insecticides alone in three of five trials. Inclusion of a penetrating surfactant at recommended rates with the insecticide and chlorothalonil fungicide did not consistently overcome this problem. Co-applications of insecticides with other fungicides did not interfere with thrips control. Co-applications of pesticides targeting multiple organisms should be examined closely to ensure that control of each organism is not compromised. To manage onion thrips in onion most effectively, insecticides should be applied with a penetrating surfactant, and should be applied separately from chlorothalonil fungicides. © 2012 Society of Chemical Industry.

  4. THE USE OF AZADIRACHTA INDICA EXTRACT TO DECREASE OF ALTERNARIA PORRI DISEASE ON ONION

    OpenAIRE

    Loso Winarto; Novia Chairuman

    2013-01-01

    Purple spot caused by Alternaria porri a major disease in the onion crop in the world. The disease is also widespread in the onion crop in Indonesia. This research aim was to know the effect of mimba leaf (Azadirachta indica) extract concentration to decrease of Alternaria porri on onion (Allium ascalonicum L).This research conducted at Research Garden of the Center of Agricultural Technology Assessment of North Sumatra Province, from October 2010 until January 2011.The researc...

  5. Quality of fresh and dry onion (Аllium cepa L. products for different

    Directory of Open Access Journals (Sweden)

    Л. Ф. Скалецька

    2013-08-01

    Full Text Available The article features results of studying suitability to processing for various varieties of onion as grown in the conditions of Ukrainian Forest-Steppe. A complex assessment has been completed for fresh products of onion for 9 different varieties by the content of basic biochemical, biological, economic, organoleptic characteristics with the view of determining the most suitable for drying. Varieties are singled out , which bulbs accumulate the biggest dry matter, sugar, C vitamin content and display the highest yield of consumable products. Assessment of dry products against the set of organoleptic and technological indicators has been completed. This revealed loss of basic biochemical components of onions during drying and long-term storage of dried products, as well as established increasing of the content of dry matters and acids and decreasing of that of sugars and vitamin C while storing the dried onion. The set of examined parameters

  6. Effects of Intercropping with Potato Onion on the Growth of Tomato and Rhizosphere Alkaline Phosphatase Genes Diversity.

    Science.gov (United States)

    Wu, Xia; Wu, Fengzhi; Zhou, Xingang; Fu, Xuepeng; Tao, Yue; Xu, Weihui; Pan, Kai; Liu, Shouwei

    2016-01-01

    In China, excessive fertilization has resulted in phosphorus (P) accumulation in most greenhouse soils. Intercropping can improve the efficiency of nutrient utilization in crop production. In this study, pot experiments were performed to investigate the effects of intercropping with potato onion (Allium cepa L. var. aggregatum G. Don) on tomato (Solanum lycopersicum L.) seedlings growth and P uptake, the diversity of rhizosphere phosphobacteria and alkaline phosphatase (ALP) genes in phosphorus-rich soil. The experiment included three treatments, namely tomato monoculture (TM), potato onion monoculture (OM), and tomato/potato onion intercropping (TI-tomato intercropping and OI-potato onion intercropping). The growth and P uptake of tomato and potato onion seedlings were evaluated. The dilution plating method was used to determine the population of phosphate-solubilizing bacteria (PSB) and phosphate-mineralizing bacteria (PMB). The genomic DNAs of PSB and PMB in the rhizosphere of tomato and potato onions were extracted and purified, and then, with the primer set of 338f /518r, the PCR amplification of partial bacterial 16S rDNA sequence was performed and sequenced to determine the diversities of PSB and PMB. After extracting the total genomic DNAs from the rhizosphere, the copy numbers and diversities of ALP genes were investigated using real-time PCR and PCR-DGGE, respectively. Intercropping with potato onion promoted the growth and P uptake of tomato seedlings, but inhibited those of potato onion. After 37 days of transplanting, compared to the rhizosphere of TM, the soil pH increased, while the electrolytic conductivity and Olsen P content decreased (p onion promoted the growth and P uptake of tomato in phosphorus-rich soil and affected the community structure and function of phosphobacteria in tomato rhizosphere. Intercropping with potato onion also improved soil quality by lowering levels of soil acidification and salinization.

  7. Effective hepatitis A virus inactivation during low-heat dehydration of contaminated green onions.

    Science.gov (United States)

    Laird, David T; Sun, Yan; Reineke, Karl F; Shieh, Y Carol

    2011-08-01

    Preserving fruits and vegetables by dehydration is common; however, information is limited concerning viral survival on the produce during the process. This work demonstrated the effects of low heat dehydration on inactivating hepatitis A virus (HAV) on contaminated green onions. Inoculated and uninoculated onion samples were dehydrated at target temperatures of 45-65 °C for 20 h. HAV from artificially contaminated onions (fresh or dehydrated) was eluted by shaking at 145 rpm at 20 °C for 20 min with 3% beef extract, pH 8, and followed by 0.2 μM-membrane filtration before plaque assay and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis. Dilutions of the filtrates were made for obtaining countable plaques on FRhK-4 cell monolayers in 6-well plates, and also for eliminating inhibitors in qRT-PCR. Average water activity of the onions after 20 h-dehydration was 0.227, regardless of temperature used (47.9 °C or 65.1 °C). Eight dehydration trials resulted in a linear relationship between HAV inactivation and dehydration temperature, with HAV log reduction = 0.1372x(°C) - 5.5572, r(2) = 0.88. Therefore, the 20 h-heating at 47.8, 55.1, and 62.4 °C reduced infectious HAV in onions by 1, 2, and 3 logs respectively, the Z value being 7.3 °C. It was concluded that low heat dehydration using 62.5 °C or above could effectively inactivate HAV on contaminated onions by >3 logs. Published by Elsevier Ltd.

  8. 7 CFR 980.501 - Safeguard procedures for potatoes, onions, and tomatoes exempt from grade, size, quality, and...

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Safeguard procedures for potatoes, onions, and....501 Safeguard procedures for potatoes, onions, and tomatoes exempt from grade, size, quality, and... imports: (1) Potatoes, onions or tomatoes for consumption by charitable institutions or distribution by...

  9. Experiences of an international trade action with irradiated onions between GDR and Hungary

    International Nuclear Information System (INIS)

    Zachariev, G.; Kiss, I.; Luther, T.; Huebner, G.; Doellstaedt, R.

    1988-01-01

    Extensive work has been carried out in the field of food irradiation in the GDR and Hungary in recent years. The irradiation of onions for sprout inhibition has reached a commercial stage in the GDR of more than 5,000 tons in 1986. The export of onions is the first example of international trade in irradiated food between socialist countries. Experiences of this trade is presented in the paper. Results of quality control of the bulbs (losses in weight an quality) after an intermediate storage period are discussed. Hungarian consumer reactions to irradiated onions is also evaluated. (author)

  10. Impact of agricultural practices on onion Banao system.

    Directory of Open Access Journals (Sweden)

    Wilfredo Valdivia Pérez

    2013-07-01

    Full Text Available In the present article was carried out a descriptive statistical study in which different tools like non structured interviews, revision of files and the use of the SIG are integrated. The investigation was developed in 2010 - 2011. The population of Banao showed a high sense of ownership to the area and 70% is in labour age highlighting the range of 25 - 39 years, out of them 24,1% of the producers have been devoted to the agriculture for 10 and 20 years. 45,8% presents problems with the supply source, 71,8% should drive the water to more than 200 m to water. When analyzing the degradation of the soil it is appreciated that 97% of the soil that is dedicated to the cultivation of the onion is eroded, existing areas where the cultivation should not be fomented, for the excessive exploitation of its soils. Among the interviewed producers 59.7% knows that there is degradation of the soils in its property, however 51.7% doesn't apply any alternative of protection of the soil. The current production of onion compromises the stability of the ecosystem considerably. This study constitutes an indispensable premise to advance toward a sustainable model of the local onion system.

  11. Drying of Agricultural Products Using Long Wave Infrared Radiation(Part 2). Drying of Welsh Onion

    International Nuclear Information System (INIS)

    Itoh, K.; Han, C.S.

    1995-01-01

    The investigation was carried out to clarify the intermittent drying characteristics for welsh onion use of long-wave infrared radiation. When compared with two other methods: use of air and vacuum freezing, this method showed significantly high rate of drying. The experiments were carried out analyzing the influence of different lengths of the welsh onion, different rate of radiation and different temperature of the airflow. The obtained results were as follows: 1. The rate of drying increases as the length of welsh onion decrease and the rate of radiation increase. 2. The airflow, temperature does not influence to the rate of drying. 3. The increasing of the drying time considerably aggravate the quality the dried welsh onion

  12. Synthesis of SrCrO4 nanostructures by onion inner-coat template ...

    Indian Academy of Sciences (India)

    Wintec

    This paper describes a bio-template method of the squama inner coat of onion for preparing the ... able to make SrCrO4 nanoparticles with average diameters in the range of 90–170 ... Up to now, considerable efforts have been focussed on ... An onion is a traditional vegetable and is available in the ... 2.3 Characterization.

  13. Impact of Pretreatment and Drying Methods on Quality Attributes of Onion Shreds

    Directory of Open Access Journals (Sweden)

    Nihar R. Sahoo

    2015-01-01

    Full Text Available Experiments were conducted on dry untreated onion shreds (2 mm thickness or treated with salt (5 % solution and potassium metabisulphite (0.5 % solution in convective drier at 50 °C ((46±4 % relative humidity (RH, 55 °C ((35±4 % RH, 60 °C ((28±4 % RH and 65 °C ((20±4 % RH, heat pump-assisted convective drier at 35 °C ((32±2 % RH, 40 °C ((26±2 % RH, 45 °C ((19±2 % RH and 50 °C ((15±2 % RH and microwave-assisted convective drier at four microwave power levels, i.e. 120, 240, 360 and 480 W. The quality parameters of the dried onion shreds, namely rehydration ratio, colour diff erence, pyruvic and ascorbic acid contents and sensory scores were evaluated. The quality of dehydrated onion shreds was observed to be comparatively better when treated in heat pump drier at 50 °C, followed by that in microwave-assisted convective drier at 240 W and 50 °C, and last in convective drier at 60 °C. The onion shreds pretreated with potassium metabisulphite retained better colour of the dried product irrespective of drying methods. Therefore, heat pump drying may be recommended as one of the best drying methods for onion shreds, because it maintains the fi nal product quality, which has practical importance for the food industry.

  14. Agronomic Evaluation of Bunching Onion in the Colombian Cundiboyacense High Plateau

    Directory of Open Access Journals (Sweden)

    Carlos H. Galeano Mendoza

    2018-01-01

    Full Text Available Bunching onion (Allium fistulosum L. is a strategic crop for Colombia due to its economic relevance within fresh and processed food markets, and therefore, there is a demand for high yielding genotypes adapted to specific regions. For this reason, after carrying out a clonal selection process including 62 genotypes, ten of these, including a regional control, were evaluated for six different traits in Boyacá (Colombia during 2012 and 2013. These traits were grouped into agronomic, yield, and processing categories. In general, these showed significant differences (p≤0.01 for genotypes, location, and genotypes × location interaction. Compared with the regional control and based on the multienvironmental analysis the genotypes Clone 30 and Clone 38 were the most promising new cultivars identified in this study. These two clones showed comparative advantages on earliness and yield, and they moreover showed some level of resistance to downy mildew and root rot, the most limiting diseases for Boyacá’s bunch onion farmers. Therefore, Clone 30 and Clone 38 were registered as new bunching onion cultivars for the Cundiboyacense High Plateau region under the names Corpoica Aquitania-1 and Corpoica Tota-1, respectively. Finally, further approaches and initiatives on bunching onion breeding are discussed.

  15. Free-radical scavenging capacity and antioxidant properties of some selected onions (Allium cepa L. and garlic (Allium sativum L. extracts

    Directory of Open Access Journals (Sweden)

    Noureddine Benkeblia

    2005-09-01

    Full Text Available The radical scavenging activity (RAS, chain-breaking activity, H2O2-scavenging, reducing capacity and total phenolics of four types of onions (Green onion, Yellow, Red and Purple and garlic were investigated. Total phenolics varied from 30 mg (green onion to 49 mg.100 g-1 fresh weight (garlic. Garlic extract showed the highest RAS, while green onion showed the lowest one. The chain-breaking activity of green onion extract was higher (0.48 than garlic extract (0.029. Chain-breaking activity of yellow, red and purple onion extracts was 0.19, 0.048 and 0.032 respectively. However, heating treatment (90 ºC, 3h caused an increase in this activity. Low ability of green onion extract to scavenge hydrogen peroxide was noted (35%, whereas high ability was noted in other onion and garlic extracts and ranged from 60 to 90%. The lowest reducing capacity was noted in green onion extract (18%, whereas the highest in garlic extract (196%. Statistically, high significant correlations were observed between total phenolics content and reducing power, scavenging of hydrogen peroxide and chain-breaking activity of extract

  16. Differential responses of onion and garlic against plant growth regulators

    International Nuclear Information System (INIS)

    Oozunidou, G.; Asif, M.; Giannakuola, A.; Iliass, A.

    2011-01-01

    The effects of Gibberellic acid-GA3, Prohexadione-Calcium, and Ethephon pre-harvest application on yield, biomass production, photosynthetic function, lipid peroxidation and quality characteristics of onion (Allium cepa L.) and garlic (Allium sativum L.) plants were investigated. Shoot length and biomass of onion and garlic, expressed either in fresh or dry weight, increased significantly under GA3, while a progressive decrease under Prohex-Ca and Ethephon occurred. Higher MDA (lipid peroxidation) values were recorded after Prohex-Ca and Ethephon supply on onion and garlic plants; it seems that GA3 treatment prevents lipid peroxidation as measured with the help of the TBARS method. Plants treated with Prohex-Ca and Ethephon revealed higher peroxidase activity compared to control and GA3 treated plants. Considering the results of MDA content and peroxidase activities it can be assumed that GA3 treated plants are slightly protected from the natural course of oxidative stress, which occurs during ageing as observed for control samples. The fluctuations of chlorophyll fluorescence parameters represent a general decline in chloroplasts function after plant growth regulators exposure, whereas in combination to the suppressed chlorophyll content, structural malformations of photo systems may also occur. The production of ascorbic acid, glucose and fructose content seems to be enhanced under GA3 in both species, while their values were depressed under Prohex-Ca and Ethephon. Overall, only GA3 supply leads to a vigorous onion and garlic growth and yield. (author)

  17. Oriented Onion Sowing by a Forked-Roller Sowing Unit

    Directory of Open Access Journals (Sweden)

    Aleksandr G.

    2018-03-01

    Full Text Available Introduction: The existing sowing machines do not provide a single feeding of the bulbs with a planting (sowing unit that leads to a violation of the agrotechnical requirements of planting bulbs. It is necessary to search new solutions to preserve the position of the bulbs in the furrow with the bottom down and their regularly spaced distribution. Materials and Methods: The article presents the design for a prototype for a planting machine equipped with a forked-roller sowing unit for orienting the onion-sowing into a furrow. Testing the forked-roller sowing unit were carried out on a flat area where the physical and mechanical properties of the soil were determined on the days of sowing, and the indices of the quality of the onion-sowing were determined. The study of the effect of the sowing machine speed on the quality of the onion-seed bulb landing was determined by the change in the translational speed of the sowing unit in the range of 0.8 m/s to 1.2 m/s with a variation interval of 0.1 m/s. The indicators of the quality of the planting of the bulbs were determined by the opening of the closed furrow. The results of laboratory-field studies of the planting machine prototype are presented. Results: The results of laboratory-field studies of a planting machine equipped with a forked-roller sowing unit for planting onion bulbs are presented. The optimal technological parameters are determined experimentally. It was determined the number of bulbs that are for up is 51 % and the regularity of planting by the forked-roller sowing unit – 79 %. These figures are provided at the forward speed of the planting machine VM = 0.9–1.0 m/s, the height of the fall of the bulb HA = 0.12 m, and the rotation frequency of the landing drum nБ = 0.47 c-1. Discussion and Conclusions: The use of a forked-roller sowing unit makes it possible to increase the proportion of onions planted by bottom down by 200 %, and the uniformity of planting bulbs by 19 %, in

  18. 75 FR 34345 - Sweet Onions Grown in the Walla Walla Valley of Southeast Washington and Northeast Oregon...

    Science.gov (United States)

    2010-06-17

    ... advancements in Walla Walla sweet onion production and storage techniques have extended the regular season for... of Walla Walla sweet onions, with an average production of 699 50-pound equivalents per acre. With an...; FV10-956-1 FR] Sweet Onions Grown in the Walla Walla Valley of Southeast Washington and Northeast...

  19. Impact of over-the-top broadcast applications of Racer® on onion weed control

    Science.gov (United States)

    The weed control challenges for onion production are formidable; however, these challenges are even greater for those considering organic crop production. Organic onion producers need organic herbicides that can effectively provide post-emergent weed control. Racer (registered trademark) is a poten...

  20. Colonization of Onions by Endophytic Fungi and Their Impacts on the Biology of Thrips tabaci

    Science.gov (United States)

    Muvea, Alexander M.; Meyhöfer, Rainer; Subramanian, Sevgan; Poehling, Hans-Michael; Ekesi, Sunday; Maniania, Nguya K.

    2014-01-01

    Endophytic fungi, which live within host plant tissues without causing any visible symptom of infection, are important mutualists that mediate plant–herbivore interactions. Thrips tabaci (Lindeman) is one of the key pests of onion, Allium cepa L., an economically important agricultural crop cultivated worldwide. However, information on endophyte colonization of onions, and their impacts on the biology of thrips feeding on them, is lacking. We tested the colonization of onion plants by selected fungal endophyte isolates using two inoculation methods. The effects of inoculated endophytes on T. tabaci infesting onion were also examined. Seven fungal endophytes used in our study were able to colonize onion plants either by the seed or seedling inoculation methods. Seed inoculation resulted in 1.47 times higher mean percentage post-inoculation recovery of all the endophytes tested as compared to seedling inoculation. Fewer thrips were observed on plants inoculated with Clonostachys rosea ICIPE 707, Trichoderma asperellum M2RT4, Trichoderma atroviride ICIPE 710, Trichoderma harzianum 709, Hypocrea lixii F3ST1 and Fusarium sp. ICIPE 712 isolates as compared to those inoculated with Fusarium sp. ICIPE 717 and the control treatments. Onion plants colonized by C. rosea ICIPE 707, T. asperellum M2RT4, T. atroviride ICIPE 710 and H. lixii F3ST1 had significantly lower feeding punctures as compared to the other treatments. Among the isolates tested, the lowest numbers of eggs were laid by T. tabaci on H. lixii F3ST1 and C. rosea ICIPE 707 inoculated plants. These results extend the knowledge on colonization of onions by fungal endophytes and their effects on Thrips tabaci. PMID:25254657

  1. 75 FR 18428 - Sweet Onions Grown in the Walla Walla Valley of Southeast Washington and Northeast Oregon...

    Science.gov (United States)

    2010-04-12

    ... indicated to the Committee that advancements in Walla Walla sweet onion production and storage techniques... harvested an average of 24 acres of Walla Walla sweet onions, with an average production of 699 50-pound... Service 7 CFR Part 956 [Doc. No. AMS-FV-10-0020; FV10-956-1 PR] Sweet Onions Grown in the Walla Walla...

  2. Non-destructive flavour evaluation of red onion (Allium cepa L.) ecotypes: an electronic-nose-based approach.

    Science.gov (United States)

    Russo, Mariateresa; di Sanzo, Rosa; Cefaly, Vittoria; Carabetta, Sonia; Serra, Demetrio; Fuda, Salvatore

    2013-11-15

    This work reports preliminary results on the potential of a metal oxide sensor (MOS)-based electronic nose, as a non-destructive method to discriminate three "Tropea Red Onion" PGI ecotypes (TrT, TrMC and TrA) from each other and the common red onion (RO), which is usually used to counterfeit. The signals from the sensor array were processed using a canonical discriminant function analysis (DFA) pattern recognition technique. The DFA on onion samples showed a clear separation among the four onion groups with an overall correct classification rate (CR) of 97.5%. Onion flavour is closely linked to pungency and thus to the pyruvic acid content. The e-nose analysis results are in good agreement with pyruvic acid analysis. This work demonstrated that artificial olfactory systems have potential for use as an innovative, rapid and specific non-destructive technique, and may provide a method to protect food products against counterfeiting. Copyright © 2013 Elsevier Ltd. All rights reserved.

  3. Effect of gamma radiation on post harvest storage life of onion bulb under ordinary room condition

    International Nuclear Information System (INIS)

    Jabeen, N.; Badshah, N.; Mahboob, F.; Ayub, G.

    2003-01-01

    Post harvest storage life of radiated onion bulbs harvested at different stages of maturity and stored at ordinary room temperature (30-39 degree C) was studied at the Department of Horticulture, NWFP Agricultural University, Peshawar, during August 2002. Onion bulbs were harvested at two stages i.e. drooping and complete dried leaf. Four radiation doses of 2, 4, 6 and 8 kilo rad (Kr) were applied with normal (control). The data of experiment was collected from weight loss, rot attack, sprouting, firmness and taste pungency. The results revealed that various radiation doses significantly affected weight loss, rot attack, sprouting, firmness and taste pungency. Onion bulbs radiated with 8 Kr showed minimum percent weight loss (28.59%), decrease in firmness and taste pungency. Minimum percent rot attack (6.3%) was observed in 6 and 8 Kr. Radiation doses of 4, 6 and 8 Kr showed no sprouting at all. Maximum weight loss (43.20%), rot attack (16.2%), sprouting (40.78%), decrease in firmness and taste pungency were recorded for control. The results showed that harvesting stages are statistically non-significant. The interaction between radiation doses and harvesting stages are also non-significant. Harvesting stages significantly affected weight loss, rot attack, sprouting and taste pungency. Onion bulbs of complete dried leaf stage recorded maximum percent weight loss (35.42%), percent sprouting (11.7%) and taste pungency while minimum percent weight loss (31.62%), percent sprouting (5.01%) and taste pungency was observed in onion bulbs of drooping stage. Maximum percent rot attack (10.9%) was noted in onion bulbs of drooping stage while minimum (7.3%) in onion bulbs of complete dried leaf stage. Radiation dose of 8 Kr is recommended to reduce the post-harvest losses in onion bulbs. Drooping stage comparatively prolonged the shelf life of bulb

  4. Screening and incorporation of rust resistance from Allium cepa into bunching onion (Allium fistulosum) via alien chromosome addition.

    Science.gov (United States)

    Wako, Tadayuki; Yamashita, Ken-ichiro; Tsukazaki, Hikaru; Ohara, Takayoshi; Kojima, Akio; Yaguchi, Shigenori; Shimazaki, Satoshi; Midorikawa, Naoko; Sakai, Takako; Yamauchi, Naoki; Shigyo, Masayoshi

    2015-04-01

    Bunching onion (Allium fistulosum L.; 2n = 16), bulb onion (Allium cepa L. Common onion group), and shallot (Allium cepa L. Aggregatum group) cultivars were inoculated with rust fungus, Puccinia allii, isolated from bunching onion. Bulb onions and shallots are highly resistant to rust, suggesting they would serve as useful resources for breeding rust resistant bunching onions. To identify the A. cepa chromosome(s) related to rust resistance, a complete set of eight A. fistulosum - shallot monosomic alien addition lines (MAALs) were inoculated with P. allii. At the seedling stage, FF+1A showed a high level of resistance in controlled-environment experiments, suggesting that the genes related to rust resistance could be located on shallot chromosome 1A. While MAAL, multi-chromosome addition line, and hypoallotriploid adult plants did not exhibit strong resistance to rust. In contrast to the high resistance of shallot, the addition line FF+1A+5A showed reproducibly high levels of rust resistance.

  5. Local Community's Knowledge on Onion Production, Pests and ...

    African Journals Online (AJOL)

    vegetables listed by FAO shows that onion ranks second after ... who sell their produce in local, regional and international markets. ..... Tanzania Official Seed Certification Institute. (TOSCI), a seed .... through integrated and organic horticulture.

  6. Large-scale synthesis of onion-like carbon nanoparticles by carbonization of phenolic resin

    International Nuclear Information System (INIS)

    Zhao Mu; Song Huaihe; Chen Xiaohong; Lian Wentao

    2007-01-01

    Onion-like carbon nanoparticles have been synthesized on a large scale by carbonization of phenolic-formaldehyde resin at 1000 o C with the aid of ferric nitrate (FN). The effects of FN loading content on the yield, morphology and structure of carbonized products were investigated using transmission electron microscopy (TEM), high-resolution TEM and X-ray diffraction. It was found that the onion-like carbon nanoparticles, which had a narrow size distribution ranging from 30 to 50 nm, were composed mainly of quasi-spherically concentric shells of well-aligned graphene layers with interlayer spacing of 0.336 nm. Based on the results of the investigation, the formation mechanism of onion-like carbon nanoparticles was also discussed

  7. Effect of different exposed lights on quercetin and quercetin glucoside content in onion (Allium cepa L.)

    OpenAIRE

    Ko, Eun Young; Nile, Shivraj Hariram; Sharma, Kavita; Li, Guan Hao; Park, Se Won

    2014-01-01

    Quercetin and quercetin glucosides are the major flavonols present in onion (Allium cepa L.) and are predominantly present as quercetin, quercetin-3,4′-diglucoside and quercetin-4′-glucoside. Effect of different light wavelengths on onion after harvest and storage, with fluorescent, blue, red and ultra violet light influenced the quercetin and quercetin glucosides profile. In a peeled onion, all the light treatments elevated quercetin content in bulb. Among them, particularly fluorescent ligh...

  8. Free sugar contents in onion bulbs on different cultivars and different production areas, and their changes by storage and gamma-irradiation. Free sugars in onion bulbs, 1

    Energy Technology Data Exchange (ETDEWEB)

    Nishibori, Sukie [Tokaigakuen Women' s Coll., Nagoya (Japan); Namiki, Kazuko

    1982-05-01

    Changes in the contents of free sugar in onion bulbs of different cultivars and different production areas were investigated during storage and processing by using gas-chromatography. No significant difference was observed in the total free sugar contents among the onions of different cultivars and different production areas except early season's cultivars which showed a slightly lower content of sugar. The free sugar was mainly composed with glucose (1.7 - 3.2%), fructose (1.1 - 2.5%) and sucrose (0.7 - 2.6%), and the composition was somewhat different among the onions of different cultivars and different production areas. During the storage at 4 - 5/sup 0/C, the total free suger contents gradually decreased during the first few months but more markedly to about 30% after sprouting. The middle part of onion bulbs contained more total free sugar than the other parts (middle part, 5.6 ..-->.. 3.5%, inner part, 5.2 ..-->.. 3.2%, outer part, 4.8 ..-->.. 1.5%), and the total free sugar contents in the outer part decreased markedly during the storage. The changes of the sugar contents after the freeze-drying were almost negligible, but showed significant decrease after the hot-air drying, especially for sucrose. The gamma irradiation with 5 - 50 krad gave no appreciable effects on the total free sugar contents though it seemed to make a slight increase in the sucrose content.

  9. THE USE OF AZADIRACHTA INDICA EXTRACT TO DECREASE OF ALTERNARIA PORRI DISEASE ON ONION

    Directory of Open Access Journals (Sweden)

    Loso Winarto

    2013-01-01

    Full Text Available Purple spot caused by Alternaria porri a major disease in the onion crop in the world. The disease is also widespread in the onion crop in Indonesia. This research aim was to know the effect of mimba leaf (Azadirachta indica extract concentration to decrease of Alternaria porri on onion (Allium ascalonicum L.This research conducted at Research Garden of the Center of Agricultural Technology Assessment of North Sumatra Province, from October 2010 until January 2011.The research used non factorial Block Randomized Design (BRD and four replications.The treatment consisted of six levels of Azadirachta indica which is dissolved in one litre of water each treatment. The number of replication are four each treatment. Treatment applied were 0 g plot-1 (control; 100 g plot-1; ; 150 g plot-1 ; 200 g plot-1 ; 250 g plot-1 ; 300 g plot-1 . The results showed that mimba leaf extract significantly affect the intensity of the purple spot disease and onion production.

  10. Studies on sprout inhibition of onions by irradiation in the Federal Republic of Germany

    International Nuclear Information System (INIS)

    Gruenewald, T.

    1978-01-01

    The Federal Republic of Germany imports about 97% of the onions for human consumption, especially from the Netherlands and from 7 other countries. The onions are distributed for sale immediately without long storage in storehouses. Therefore the objective of this study was not primarily to clear the irradiation and storage processing but to obtain better information about the quality of imported irradiated onions and about the parameters influencing the quality. Seven varieties cultivated in Germany with different physical and sensory properties were irradiated with 10MeV electrons at doses of 5 or 10krad and stored at temperatures of +4, +9, +10 or +20 0 C. The time between harvest and irradiation and the storage conditions for this time were also varied. In all periods physical, chemical and sensory properties of fresh and cooked bulbs were tested and statistically analysed during the storage periods of 8 to 10 months after harvest. It could be demonstrated that a dose of 5krad of 10MeV electrons was high enough to inhibit sprouting in all varieties, independent of the storage conditions, if the irradiation was applied within 4 weeks after harvest. If more time elapsed between harvest and irradiation, reduced sprout suppression was obtained. The sensory quality of the onion bulbs, irradiated and stored for 8 months, was evaluated as only 1 or 2 points lower than at the beginning of storage, when tested as cooked vegetables. The influence of the variety and storage conditions on the properties was more evident than the influence of irradiation, apart from sprouting. However, the browning in the growth centre of the irradiated onion bulbs could not be ignored. Varying the time between harvest and irradiation had no effect, and different storage conditions demonstrated only that lower storage temperatures delayed the browning but did not prevent it. This will probably not influence the marketability of the onions and would be compensated by lower prices for the

  11. Nitrogen Transfer from Cover Crop Residues to Onion Grown under Minimum Tillage in Southern Brazil

    Directory of Open Access Journals (Sweden)

    Leoncio de Paula Koucher

    2017-08-01

    Full Text Available ABSTRACT Nitrogen derived from cover crop residues may contribute to the nutrition of onion grown under minimum tillage (MT and cultivated in rotation. The aim of this study was to evaluate the N transferred from different cover crop residues to the onion crop cultivated under MT in southern Brazil. In June 2014, oilseed radish, black oat, and oilseed radish + black oat residues labeled with 15N were deposited on the soil surface before transplanting onions. During the growth season and at harvest, young expanded onion leaves, complete plants, and samples from different soil layers were collected and analyzed for recovery of 15N-labeled residue. Oilseed radish decomposed faster than other residues and 4 % of residue N was recovered in leaves and bulbs at harvest, but in general, N in plant organs was derived from sources other than the cover crop residues. In addition, leaf N was in the proper range for all treatments and was adequately mobilized to the bases for bulbing. The N derived from decomposing residues contributed little to onion development and the use of these plants should be chosen based on their advantages for physical and biological soil quality.

  12. Garlic and onions: Their cancer prevention properties

    Science.gov (United States)

    Nicastro, Holly L.; Ross, Sharon A.; Milner, John A.

    2015-01-01

    The Allium genus includes garlic, onions, shallots, leeks, and chives. These vegetables are popular in cuisines worldwide and are valued for their potential medicinal properties. Epidemiological studies, while limited in their abilities to assess Allium consumption, indicate some associations of Allium vegetable consumption with decreased risk of cancer, particularly cancers of the gastrointestinal tract. Limited intervention studies have been conducted to support these associations. The majority of supportive evidence on Allium vegetables cancer preventive effects comes from mechanistic studies. These studies highlight potential mechanisms of individual sulfur-containing compounds and of various preparations and extracts of these vegetables, including decreased bioactivation of carcinogens, antimicrobial activities, and redox modification. Allium vegetables and their components have effects at each stage of carcinogenesis and affect many biological processes that modify cancer risk. This review discusses the cancer preventive effects of Allium vegetables, particularly garlic and onions, and their bioactive sulfur compounds and highlights research gaps. PMID:25586902

  13. Comparative assessment of onion and garlic extracts on endogenous hepatic and renal antioxidant status in rat.

    Science.gov (United States)

    Suru, Stephen M; Ugwu, Chidiebere E

    2015-07-01

    Despite growing claims of functional health benefits in folkloric medicine, the safety of chronic/elevated intakes of onion and garlic cannot be assumed. Therefore, this study assesses oral administration of varied doses of onion and garlic on some biomarkers of hepatic and renal functions in rats. Animals were divided into five groups: control group received vehicle and extract-treated groups received varied doses of onion or garlic extract (0.5 mL and 1.0 mL/100 g bwt/day) for 6 weeks. Both doses of onion caused marked (pgarlic elicited similar trend except in hepatic CAT, renal SOD and GST levels. A high dose of garlic only caused marked (pgarlic significantly (pgarlic, while high dose of garlic elicited pro-oxidant conditions.

  14. HS-SPME-GC-MS Analysis of onion (Allium cepa L. and shallot (Allium ascalonicum L.

    Directory of Open Access Journals (Sweden)

    D’Auria, M.

    2017-06-01

    Full Text Available The volatile organic compounds of onion and shallot were determined via HS-SPME-GC-MS. The main components were dipropyldisulphide and allylpropyldisulphide. Thiopropanal S-oxide were detected only in onion volatiles. In shallot is interesting the presence of 2-methyl-2-pentenal, a compound with an intense fruity aroma, that can characterize the different aroma between onion and shallot. The SPME-GC-MS analysis of shallot after absorption on the SPME fiber at 50°C showed the presence of new compounds, whose structures have been discussed.

  15. Effects of Onion Extracts on Growth Performance, Carcass Characteristics and Blood Profiles of White Mini Broilers

    Directory of Open Access Journals (Sweden)

    B. K. An

    2015-02-01

    Full Text Available This experiment was carried out to investigate effects of onion extract on growth performance, meat quality and blood profiles of White mini broilers. Total of 600 one-d-old male White mini broiler chicks were divided into four groups and fed control diets (non-medicated commercial diet or antibiotics medicated or experimental diets (non-medicated diets containing 0.3% or 0.5% onion extract for 5 wks. The final body weight (BW and weight gain of the group fed non-medicated control diet were lower than those of medicated control group (p<0.01. The chicks fed diet with 0.3% or 0.5% onion extract showed a similar BW to that of medicated control group. The relative weight of various organs, such as liver, spleen, bursa of Fabricius, abdominal fat, and the activities of serum enzymes were not affected by dietary treatments. There were no significant differences in meat color among groups. Whereas, groups fed diets containing onion extract had slightly lower cooking loss and higher shear force value, but not significantly. The concentrations of serum free cholesterol and triacylglycerol in groups fed diet containing onion extract were significantly decreased compared with those of controls (p<0.01. In conclusion, the onion extracts exerted a growth-promoting effect when added in White mini broiler diets, reflecting potential alternative substances to replace antibiotics.

  16. Pantoea ananatis Genetic Diversity Analysis Reveals Limited Genomic Diversity as Well as Accessory Genes Correlated with Onion Pathogenicity

    Directory of Open Access Journals (Sweden)

    Shaun P. Stice

    2018-02-01

    Full Text Available Pantoea ananatis is a member of the family Enterobacteriaceae and an enigmatic plant pathogen with a broad host range. Although P. ananatis strains can be aggressive on onion causing foliar necrosis and onion center rot, previous genomic analysis has shown that P. ananatis lacks the primary virulence secretion systems associated with other plant pathogens. We assessed a collection of fifty P. ananatis strains collected from Georgia over three decades to determine genetic factors that correlated with onion pathogenic potential. Previous genetic analysis studies have compared strains isolated from different hosts with varying diseases potential and isolation sources. Strains varied greatly in their pathogenic potential and aggressiveness on different cultivated Allium species like onion, leek, shallot, and chive. Using multi-locus sequence analysis (MLSA and repetitive extragenic palindrome repeat (rep-PCR techniques, we did not observe any correlation between onion pathogenic potential and genetic diversity among strains. Whole genome sequencing and pan-genomic analysis of a sub-set of 10 strains aided in the identification of a novel series of genetic regions, likely plasmid borne, and correlating with onion pathogenicity observed on single contigs of the genetic assemblies. We named these loci Onion Virulence Regions (OVR A-D. The OVR loci contain genes involved in redox regulation as well as pectate lyase and rhamnogalacturonase genes. Previous studies have not identified distinct genetic loci or plasmids correlating with onion foliar pathogenicity or pathogenicity on a single host pathosystem. The lack of focus on a single host system for this phytopathgenic disease necessitates the pan-genomic analysis performed in this study.

  17. Proteomic Characterization of Differential Abundant Proteins Accumulated between Lower and Upper Epidermises of Fleshy Scales in Onion (Allium cepa L. Bulbs.

    Directory of Open Access Journals (Sweden)

    Si Wu

    Full Text Available The onion (Allium cepa L. is widely planted worldwide as a valuable vegetable crop. The scales of an onion bulb are a modified type of leaf. The one-layer-cell epidermis of onion scales is commonly used as a model experimental material in botany and molecular biology. The lower epidermis (LE and upper epidermis (UE of onion scales display obvious differences in microscopic structure, cell differentiation and pigment synthesis; however, associated proteomic differences are unclear. LE and UE can be easily sampled as single-layer-cell tissues for comparative proteomic analysis. In this study, a proteomic approach based on 2-DE and mass spectrometry (MS was applied to compare LE and UE of fleshy scales from yellow and red onions. We identified 47 differential abundant protein spots (representing 31 unique proteins between LE and UE in red and yellow onions. These proteins are mainly involved in pigment synthesis, stress response, and cell division. Particularly, the differentially accumulated chalcone-flavanone isomerase and flavone O-methyltransferase 1-like in LE may result in the differences in the onion scale color between red and yellow onions. Moreover, stress-related proteins abundantly accumulated in both LE and UE. In addition, the differential accumulation of UDP-arabinopyranose mutase 1-like protein and β-1,3-glucanase in the LE may be related to the different cell sizes between LE and UE of the two types of onion. The data derived from this study provides new insight into the differences in differentiation and developmental processes between onion epidermises. This study may also make a contribution to onion breeding, such as improving resistances and changing colors.

  18. Calorie Restriction Effect of Heat-Processed Onion Extract (ONI Using In Vitro and In Vivo Animal Models

    Directory of Open Access Journals (Sweden)

    Yu-Ri Kang

    2018-03-01

    Full Text Available Onion (Allium cepa L. is widely consumed as food or medicinal plant due to its well-defined health benefits. The antioxidant and antihyperlipidemic effects of onion and its extracts have been reported well. However, very limited information on anti-hyperglycemic effect is available in processed onion extracts. In our previous study, we reported that Amadori rearrangement compounds (ARCs produced by heat-processing in Korean ginseng can reduce carbohydrate absorption by inhibiting intestinal carbohydrate hydrolyzing enzymes in both in vitro and in vivo animal models. To prove the enhancement of anti-hyperglycemic effect and ARCs content by heat-processing in onion extract, a correlation between the anti-hyperglycemic activity and the total content of ARCs of heat-processed onion extract (ONI was investigated. ONI has a high content of ARCs and had high rat small intestinal sucrase inhibitory activity (0.34 ± 0.03 mg/mL, IC50 relevant for the potential management of postprandial hyperglycemia. The effect of ONI on the postprandial blood glucose increase was investigated in Sprague Dawley (SD rats fed on sucrose or starch meals. The maximum blood glucose levels (Cmax of heat-processed onion extract were significantly decreased by about 8.7% (from 188.60 ± 5.37 to 172.27 ± 3.96, p < 0.001 and 14.2% (from 204.04 ± 8.73 to 175.13 ± 14.09, p < 0.01 in sucrose and starch loading tests, respectively. These results indicate that ARCs in onion extract produced by heat-processing have anti-diabetic effect by suppressing carbohydrate absorption via inhibition of intestinal sucrase, thereby reducing the postprandial increase of blood glucose. Therefore, enhancement of ARCs in onion by heat-processing might be a good strategy for the development of the new product on the management of hyperglycemia.

  19. Temporal dynamics of iris yellow spot virus and its vector, Thrips tabaci (Thysanoptera: Thripidae), in seeded and transplanted onion fields.

    Science.gov (United States)

    Hsu, Cynthia L; Hoepting, Christine A; Fuchs, Marc; Shelton, Anthony M; Nault, Brian A

    2010-04-01

    Onion thrips, Thrips tabaci (Lindeman) (Thysanoptera: Thripidae), can reduce onion bulb yield and transmit iris yellow spot virus (IYSV) (Bunyaviridae: Tospovirus), which can cause additional yield losses. In New York, onions are planted using seeds and imported transplants. IYSV is not seed transmitted, but infected transplants have been found in other U.S. states. Transplants are also larger than seeded onions early in the season, and thrips, some of which may be viruliferous, may preferentially colonize larger plants. Limited information is available on the temporal dynamics of IYSV and its vector in onion fields. In 2007 and 2008, T. tabaci and IYSV levels were monitored in six seeded and six transplanted fields. We found significantly more thrips in transplanted fields early in the season, but by the end of the season seeded fields had higher levels of IYSV. The percentage of sample sites with IYSV-infected plants remained low (fields. The densities of adult and larval thrips in August and September were better predictors of final IYSV levels than early season thrips densities. For 2007 and 2008, the time onions were harvested may have been more important in determining IYSV levels than whether the onions were seeded or transplanted. Viruliferous thrips emigrating from harvested onion fields into nonharvested ones may be increasing the primary spread of IYSV in late-harvested onions. Managing T. tabaci populations before harvest, and manipulating the spatial arrangement of fields based on harvest date could mitigate the spread of IYSV.

  20. An onion farmer with a case of hypersensitivity pneumonitis caused by Aspergillus niger.

    Science.gov (United States)

    Sakamoto, Tomohiro; Yamasaki, Akira; Funaki, Yoshihiro; Harada, Tomoya; Okazaki, Ryota; Hasegawa, Yasuyuki; Sueda, Yuriko; Fukushima, Takehito; Morita, Masahito; Yamamoto, Akihiro; Kodani, Masahiro; Shimizu, Eiji

    2018-01-01

    A 62-year old man was admitted to our hospital with a 2-week complaint of a dry cough, general fatigue, and dyspnea on effort. He has been an onion farmer for several years and developed these symptoms after cleaning up onion peels with air compressors. A chest roentgenogram and computed tomography showed a ground glass shadow in the bilateral upper lung field. Cellular analysis of the bronchoalveolar lavage fluid showed elevated total cell numbers and lymphocytes. Transbronchial lung biopsies revealed a non-caseating granuloma with both epithelioid cells and Langhans giant cells. After the admission, these symptoms and radiological findings gradually improved without any treatment. Then, a returning-home provocation test was positive only when he worked cleaning up onion peels with air compressors. A. niger was cultured from his workplace and black mold from the onion peels. The precipitation antibody and the antigen were both positive for Aspergillus . Therefore, we diagnosed this case as hypersensitivity pneumonitis caused by inhalation of A. niger . Although hypersensitivity pneumonia caused by A. niger is rare, physicians should aware the possibility of this condition in farmers because A niger is ubiquitously present in several vegetables and fruits.

  1. Conservation of onions using gamma radiation

    International Nuclear Information System (INIS)

    Wiendl, Frederico M.; Arthur, Valter

    1999-01-01

    An increase of shelf life of onions (Var. Valencia Sintetica-14) irradiated with doses from 20 and 80 Gy was observed and this increase varied from 30 per cent to 50 per cent of the irradiated bulbs in relation to the controls. The smallest diminishment on daily weight was achieved with doses of 46.46 Gy and 67.30 Gy calculated through regression analysis on the first and second experiments respectively. In relation to the discarded onions, two parameters were taken into account: external visible sprouting and rotting. The sprouting decreased from 58.3 per cent in the controls down to 0 per cent at the doses of 80 and 120 Gy. The rotting was 41.7 per cent in the controls and increased to 100 per cent at the doses of 80 and 120 Gy respectively after 382 and 262 days by an increase of shelf life of 75.2 per cent and 29.1 per cent on both the experiments. The best dose for prolonging shelf life was considered to be 40 Gy reaching a 90.4 per cent longer life. (author)

  2. Evaluation of the quality of hot air dehydrated onion coming from gamma radiated bulbs

    International Nuclear Information System (INIS)

    Elman, L.; Pezzutti, A.; Croci, C.A.

    2003-01-01

    The purpose of this work was to evaluate the quality of hot air dehydrated onion, as regards physical and chemical characteristics, coming from the regional product that was gamma irradiated for sprout inhibition. We worked with the onion variety Valenciana Sintetica 14. Radio inhibition was made 30 days post harvest with gamma radiation from a 60 Co source at the Centro Atomico Ezeiza-CNEA, using a dose of 60 Gy. The skin of the bulbs was manually removed and the bulbs were cut in pieces 3 mm thick and between 1 and 3 cm long. The material was dehydrated in a rotating dryer with forced air circulation at 60 C degrees, between 0.8 and 1.7 m/s air speed and at ambient relative humidity. Dehydration was made 80 days after post-irradiation. The quality of the dehydrated onion was evaluated by the following physical- chemical analysis: total solids content, pungency (indirectly measured by pyruvic acid content assessment), color, pH, carbon hydrates and sensorial analysis. All analytical determinations were made in triplicate. The results obtained showed there are no significant changes between the averages of the physical-chemical properties of the control dehydrated samples and those coming from the radio-inhibited raw matter. According to the sensorial analysis, only the color of dehydrated onion was affected by the radio inhibition process. However, and according to the panel members comment, the greatest browning degree observed in ionizing radiation treated onion seemed to result more attractive to them. It may be concluded that radio inhibited regional onion can be useful as raw matter for hot air dehydrated product. It must be remarked that its use would extend the product use by dehydration plants, thus implying an increase of their processing capacity with the corresponding financial benefit. (author)

  3. Estimation of optical constants of a bio-thin layer (onion epidermis), using SPR spectroscopy

    International Nuclear Information System (INIS)

    Rehman, Saif-ur-; Hayashi, Shinji; Sekkat, Zouheir; Mumtaz, Huma; Shaukat, S F

    2014-01-01

    We estimate the optical constants of a biological thin layer (Allium cepa) by surface plasmon resonance (SPR) spectroscopy. For this study, the fresh inner thin epidermis of an onion bulb was used and stacked directly on gold (Au) and silver (Ag) film surfaces in order to identify the shift in SPR mode of each metal film at an operating wavelength of 632.8 nm. The thickness and dielectric constants of the biological thin layer were determined by matching the experimental SPR curves to theoretical ones. The thickness and roughness of bare Au and Ag thin films were also measured by atomic force microscopy (AFM); the results of which are in good agreement with those obtained through experiment. Due to the high surface roughness of the natural onion epidermis layer, AFM could not measure the exact thickness of an onion epidermis. It is estimated that the value of the real part of the dielectric constant of an onion epidermis is between the dielectric constants of water and air. (paper)

  4. Effects of gamma radiation on the storage of domestic onions under natural conditions

    International Nuclear Information System (INIS)

    Ayyoubi, Zouhair; Sharabi, N.E.

    1993-02-01

    Local variety (red and white onions) were stored under natural conditions using gamma radiation. 30, 60, 90 and 120 Gy of doses were used in three replicates with the control. Onions were irradiated after fifteen days of harvesting. Weight reduction, internal and external sprout were recorded monthly during the storage which lasted for eight months. The results proved a positive effect of radiation in all the doses used in sprout inhibition and accepted effect statistically in limiting the loss of weight. Decrease in the occurrence and intensity of disease and insect infection have been noticed in the treatments in comparison with the control. Therefore, doses of 60 and 90 Gy have been considered to be the best in storing onions under the conditions of the experiments. (author). 5 refs., 2 figs

  5. Application of indigenously prepared graphene oxide on onion in combination with gamma irradiation

    International Nuclear Information System (INIS)

    Khade, H.D.; Gadly, T.; Jain, M.P.; Ghosh, S.K.

    2017-01-01

    Onion (Allium cepa), a commonly used vegetable, it ranks third in the global production of major vegetables. Due to its delicious taste, flavour and pungency used in many culinary preparations and it has several medicinal properties also. The preservation of onion is a major thrust area since a long ago. In this study farm fresh onions of kharif season were harvested from farms nearby town of Dist. Satara, Maharashtra and were used for study. Variety named Lonand (Agri Dark Red (ADR)) were selected for study. Graphene oxide was sprayed and dried at ambient temperature for half an hour. Suitably packed in nylon mesh bag having capacity 3 Kg. Gamma radiation dose 70 Gy were given at Food package irradiator, FIPLY (dose rate 3.5 Gy/Min) at the centre

  6. Towards onions and shallots (Allium cepa L.) resistant to beet armyworm (Spodoptera exigua Hübner) by transgenesis and conventional breeding

    NARCIS (Netherlands)

    Zheng, S.J.

    2000-01-01

    Onion ( Allium cepa L. group Common Onion) and shallot ( A. cepa L. group Aggregatum) are two subspecies of A. cepa . Both onion and shallot together with other Allium species like garlic ( A.

  7. Combined Effect of Kimchi Powder and Onion Peel Extract on Quality Characteristics of Emulsion Sausages Prepared with Irradiated Pork

    Science.gov (United States)

    Choi, Yun-Sang; Lee, Ju-Woon; Lee, Si-Kyung

    2015-01-01

    This study was conducted to investigate the effects of kimchi powder and onion peel extract on the quality characteristics of emulsion sausage manufactured with irradiated pork. The emulsion sausages were formulated with 2% kimchi powder and/or 0.05% onion peel extract. The changes in pH value of all treatments were similar, depending on storage periods. The addition of kimchi powder increased the redness and yellowness of the emulsion sausage. The addition of onion peel extract decreased the thiobarbituric acid reactive substances value of the emulsion sausages prepared with irradiated pork. The volatile basic nitrogen value of the emulsion sausage prepared with kimchi powder was the highest, whereas that of the emulsion sausage prepared with onion peel extract was the lowest. The treatment without kimchi powder or onion peel extract and the treatments prepared with onion peel extract showed lower microbial populations than the other treatment. Sensory evaluations indicated that a higher acceptability was attained when kimchi powder was added to the emulsion sausages manufactured with irradiated pork. In conclusion, our results suggest that combined use of kimchi powder and onion peel extract could improve quality characteristics and shelf stability of the emulsion sausage formulated with irradiated pork during chilled storage. PMID:26761840

  8. A Rapid, Highly Efficient and Economical Method of Agrobacterium-Mediated In planta Transient Transformation in Living Onion Epidermis

    Science.gov (United States)

    Xu, Kedong; Huang, Xiaohui; Wu, Manman; Wang, Yan; Chang, Yunxia; Liu, Kun; Zhang, Ju; Zhang, Yi; Zhang, Fuli; Yi, Liming; Li, Tingting; Wang, Ruiyue; Tan, Guangxuan; Li, Chengwei

    2014-01-01

    Transient transformation is simpler, more efficient and economical in analyzing protein subcellular localization than stable transformation. Fluorescent fusion proteins were often used in transient transformation to follow the in vivo behavior of proteins. Onion epidermis, which has large, living and transparent cells in a monolayer, is suitable to visualize fluorescent fusion proteins. The often used transient transformation methods included particle bombardment, protoplast transfection and Agrobacterium-mediated transformation. Particle bombardment in onion epidermis was successfully established, however, it was expensive, biolistic equipment dependent and with low transformation efficiency. We developed a highly efficient in planta transient transformation method in onion epidermis by using a special agroinfiltration method, which could be fulfilled within 5 days from the pretreatment of onion bulb to the best time-point for analyzing gene expression. The transformation conditions were optimized to achieve 43.87% transformation efficiency in living onion epidermis. The developed method has advantages in cost, time-consuming, equipment dependency and transformation efficiency in contrast with those methods of particle bombardment in onion epidermal cells, protoplast transfection and Agrobacterium-mediated transient transformation in leaf epidermal cells of other plants. It will facilitate the analysis of protein subcellular localization on a large scale. PMID:24416168

  9. Study on the Extraction and Application of Natural Dyestuff from Onion Skin

    International Nuclear Information System (INIS)

    Nwe Yee Win; Moe Moe Swe

    2010-12-01

    The revival of the use of natural dyes on textiles is due to the increased environmental pollution today. Because of their non-toxic, biodegradable properties and health hazards associated with the synthetic dyes, there is a global interest in natural dyed textiles. This research mainly focuses on the extraction and application of natural dyestuff from onion skin which can be easily collected in Myanmar. The objectives of this study are to extract natural dyestuff from onion skin by studying the effect of dye extraction mediums, to study the optimum dye extraction condition, and to study the fastness properties on dyed sample material. Preliminary phytochemical tests are performed to determine the compounds present in the onion skin. The best dye extraction method is determined by using four types of extraction mediums.Then the determination of dye extraction conditions is investigated by studying the effect of extraction time and solvent ratio on dye yield percent. After that the dye powder extract is applied on cotton fabrics by using different types of fixing agents. Among them, the most suitable type of mordant with onion skin dyestuff is selected for further study. Fastness properties on dyed cotton fabrics such as light, rubbing and washing are carried out. And then the range of colour developed on dyed materials is studied by CIE L*a* b* colour coordinates

  10. Production of Quorum Sensing Inhibitors in Growing Onion Bulbs Infected with Pseudomonas aeruginosa E (HQ324110).

    Science.gov (United States)

    Abd-Alla, Mohamed H; Bashandy, Shymaa R

    2012-01-01

    Eighteen organic compounds were present in growing onion bulbs cultivar Giza 6 infected with P. aeruginosa, but only fourteen of them are present in dry infected onion bulbs; however, four compounds were missing in dry onion. The missing compounds in dry infected onion bulbs are pantolactone, 4,5-dihydro-4,5-dimethylfuran-2(3H)-one, myristic acid, and linoleic acid. All of them were detected in growing onion (living cell) during Pseudomonas aeruginosa infection, and it is hypothesized that it may be produced by plants and act as defence system. Pantolactone and myristic acid were selected to explore their effects on growth and virulence factors of Pseudomonas aeruginosa. Exogenous application of pantolactone and myristic acid significantly inhibited pyocyanin production, protease, and lipase and polygalacturonase activity but did not have any significant effects on bacterial growth. The inhibition of virulence factors without reduction in bacterial growth may be providing strong support that these chemical molecules are general quorum sensing inhibitors than an antibacterial effect. Disruption of quorum sensing of pathogen indicates that this new approach has potential in fighting bacterial infections in human and plants.

  11. Organic cultivation of onion under castor cake fertilization and irrigation depths

    Directory of Open Access Journals (Sweden)

    Gabriel Alves Botelho de Mello

    2017-11-01

    Full Text Available Two experiments were carried out to evaluate the effects that different doses of castor bean (0, 200, and 300 g m-2 and irrigation levels (204, 224, 278, and 321 mm in 2014, and 278, 302, 397, and 444 mm in 2015 have on both the productivity and quality of onion bulbs. In the experiments, the experimental design was randomized blocks (4 x 3 factorial scheme, with five replicates. Irrigation management was performed using a Simplified Irrigation Device (SID in response to soil water tension in the treatment of highest irrigation depth. The following variables were evaluated: plant dry biomass (PDB, bulb dry biomass (BDB, total yield (TY, mean bulb fresh weight (MBFW, mean bulb dry weight (MBDW, mean bulb diameter (MBD and water use efficiency (WUE. The highest irrigation depths positively influenced the mean production of onion bulbs, regardless of the applied dose of castor cake. The doses of castor cake positively influenced the production of onion bulbs when higher irrigation depths were applied.

  12. Effect of harvest time on storage loss and sprouting in onion

    Directory of Open Access Journals (Sweden)

    T. SUOJALA

    2008-12-01

    Full Text Available Storability of onion is affected by timing of harvest. However, the optimal time for maximum yield and maximum storability do not necessarily coincide. This study aimed to determine the most suitable harvest time for obtaining a high bulb yield with high quality and storability. Storage experiments were conducted on onions produced in field experiments at a research field and on farms in four years. Results indicate that harvesting could be delayed to 100% maturity, or even longer, without a marked increase in storage loss. In rainy years, late harvest is likely to impair the quality. The incidence of sprouting in shelf life tests varied considerably between years. An early harvest before 50% maturity and a delayed harvest increased the risk of sprouting. It may be concluded that the harvesting of onions for long-term storage can be timed to take place between 50% maturity and even some weeks after complete maturity without a loss in storage quality. Therefore, it is possible to combine high yield and good storage quality.

  13. Micropropagation of onion (Allium cepa L.) from immature inflorescences.

    Science.gov (United States)

    Marinangeli, Pablo

    2013-01-01

    In vitro plant production by direct organogenesis from immature flower heads is an ideal approach for clonal propagation of onions (Allium cepa L.). This technique ensures genetic stability, high propagation rate, and maintains donor plant of explants with an advantage over other means of in vitro regeneration. Onion micropropagation is usually applied in breeding programs, maintenance, and multiplication of cytoplasmic-male sterile lines for hybrid production, germplasm conservation, and as a tool for the application of other biotechnologies. For in vitro culture, mature onion bulbs are induced to reproductive phase by vernalization and forced to inflorescence initiation. Immature umbels are dissected from bulbs or cut directly when they appear from the pseudostem among the leaves. Disinfected inflorescences are cultivated in BDS basal medium supplemented with 30 g/L sucrose, 0.1 mg/L naphthalene acetic acid, 1 mg/L N (6)-benzyladenine, and 8 g/L agar, pH 5.5, under 16 h photoperiod white fluorescent light (PPD: 50-70 μmol/m(2)s) for 35 days. The regenerated shoot clumps are divided and subculture under the same conditions. For bulbification phase, the individual shoots are cultured in BDS basal medium containing 90 g/L sucrose, without plant growth regulators, pH 5.5, under 16 h photoperiod. Microbulbs can be directly cultivated ex vitro without acclimation.

  14. Protective Effect of Onion Extract on Bleomycin-Induced Cytotoxicity and Genotoxicity in Human Lymphocytes

    Directory of Open Access Journals (Sweden)

    Yoon Hee Cho

    2016-02-01

    Full Text Available Following one of the world’s largest nuclear accidents, occured at Fukushima, Japan in 2011, a significant scientific effort has focused on minimizing the potential adverse health effects due to radiation exposure. The use of natural dietary antioxidants to reduce the risk of radiation-induced oxidative DNA damage is a simple strategy for minimizing radiation-related cancer rates and improving overall health. The onion is among the richest sources of dietary flavonoids and is an important food for increasing their overall intake. Therefore, we examined the effect of an onion extract on cyto- and geno-toxicity in human lymphocytes treated with bleomycin (BLM, a radiomimetic agent. In addition, we measured the frequency of micronuclei (MN and DNA damage following treatment with BLM using a cytokinesis-blocked micronucleus assay and a single cell gel electrophoresis assay. We observed a significant increase in cell viability in lymphocytes treated with onion extract then exposed to BLM compared to cells treated with BLM alone. The frequency of BLM induced MN and DNA damage increased in a dose-dependent manner; however, when lymphocytes were pretreated with onion extract (10 and 20 μL/mL, the frequency of BLM-induced MN was decreased at all doses of BLM and DNA damage was decreased at 3 μg/mL of BLM. These results suggest that onion extract may have protective effects against BLM-induced cyto- and genotoxicity in human lymphocytes.

  15. The L locus, one of complementary genes required for anthocyanin production in onions (Allium cepa), encodes anthocyanidin synthase.

    Science.gov (United States)

    Kim, Sunggil; Jones, Rick; Yoo, Kil-Sun; Pike, Leonard M

    2005-06-01

    Bulb color in onions (Allium cepa) is an important trait, but its complex, unclear mechanism of inheritance has been a limiting factor in onion cultivar improvement. The identity of the L locus, which is involved in the color difference between Brazilian yellow and red onions, is revealed in this study. A cross was made between a US-type yellow breeding line and a Brazilian yellow cultivar. The segregation ratio of nine red to seven yellow onions in the F(2) population supports the involvement of two complementary genes in anthocyanin production in the F(1) hybrids. The high-performance liquid chromatography (HPLC) and reverse-transcriptase (RT)-PCR analysis of the Brazilian yellow onions indicated that the genes are involved late in the anthocyanin synthesis pathway. The genomic sequence of the anthocyanidin synthase (ANS) gene in Brazilian yellow onions showed a point mutation, which results in an amino acid change of a glycine to an arginine at residue 229. Because this residue is located adjacent to a highly conserved iron-binding active site, this mutation is likely responsible for the inactivation of the ANS gene in Brazilian yellow onions. Following the isolation of the promoter sequence of the mutant allele, a PCR-based marker for allelic selection of the ANS gene was designed. This assay is based on an insertion (larger than 3 kb) mutation. The marker perfectly co-segregated with the color phenotypes in the F(2) populations, thereby indicating that the L locus encodes ANS.

  16. Sequencing and annotation of the chloroplast DNAs and identification of polymorphisms distinguishing normal male-fertile and male-sterile cytoplasms of onion.

    Science.gov (United States)

    von Kohn, Christopher; Kiełkowska, Agnieszka; Havey, Michael J

    2013-12-01

    Male-sterile (S) cytoplasm of onion is an alien cytoplasm introgressed into onion in antiquity and is widely used for hybrid seed production. Owing to the biennial generation time of onion, classical crossing takes at least 4 years to classify cytoplasms as S or normal (N) male-fertile. Molecular markers in the organellar DNAs that distinguish N and S cytoplasms are useful to reduce the time required to classify onion cytoplasms. In this research, we completed next-generation sequencing of the chloroplast DNAs of N- and S-cytoplasmic onions; we assembled and annotated the genomes in addition to identifying polymorphisms that distinguish these cytoplasms. The sizes (153 538 and 153 355 base pairs) and GC contents (36.8%) were very similar for the chloroplast DNAs of N and S cytoplasms, respectively, as expected given their close phylogenetic relationship. The size difference was primarily due to small indels in intergenic regions and a deletion in the accD gene of N-cytoplasmic onion. The structures of the onion chloroplast DNAs were similar to those of most land plants with large and small single copy regions separated by inverted repeats. Twenty-eight single nucleotide polymorphisms, two polymorphic restriction-enzyme sites, and one indel distributed across 20 chloroplast genes in the large and small single copy regions were selected and validated using diverse onion populations previously classified as N or S cytoplasmic using restriction fragment length polymorphisms. Although cytoplasmic male sterility is likely associated with the mitochondrial DNA, maternal transmission of the mitochondrial and chloroplast DNAs allows for polymorphisms in either genome to be useful for classifying onion cytoplasms to aid the development of hybrid onion cultivars.

  17. Effect of γ-irradiation in the keeping quality of Nigerian onion cultivars

    International Nuclear Information System (INIS)

    Agbaji, A.S.; Ogbadu, G.H.; Amstel, H. van

    1981-01-01

    Onion of variety Ds x Ws'78 and Ds'79 harvested 14 days prior to the experiment were treated with 5, 8 and 15 krad doses of γ-irradiation from a 60 Co γ-irradiator and stored. Onions were weekly investigated for sprouting and rotting. Chemical analyses were carried out monthly for dry matter, flavour, ascorbic acid, reducing sugar and protein. In comparison, a local method of storage was carried out. Irradiation dose of 5-15 krad completely inhibited sprouting throughout the whole storage period. Irradiated bulbs however, showed a higher tendency to rot. In comparison with the control, irradiation did not have any significant effect on dry matter, flavour component and ascorbic acid but the protein content was significantly lower in the irradiated bulbs. The compositional differences found between irradiated and untreated onions seem to be of no nutritional or organoleptic significance. Almost 100% loss was recorded for local storage trial. (author)

  18. Cover Crops Effects on Soil Chemical Properties and Onion Yield

    Directory of Open Access Journals (Sweden)

    Rodolfo Assis de Oliveira

    2016-01-01

    Full Text Available ABSTRACT Cover crops contribute to nutrient cycling and may improve soil chemical properties and, consequently, increase crop yield. The aim of this study was to evaluate cover crop residue decomposition and nutrient release, and the effects of these plants on soil chemical properties and on onion (Allium cepa L. yield in a no-tillage system. The experiment was carried out in an Inceptisol in southern Brazil, where cover crops were sown in April 2012 and 2013. In July 2013, shoots of weeds (WD, black oats (BO, rye (RY, oilseed radish (RD, oilseed radish + black oats (RD + BO, and oilseed radish + rye (RD + RY were cut at ground level and part of these material from each treatment was placed in litter bags. The litter bags were distributed on the soil surface and were collected at 0, 30, 45, 60, 75, and 90 days after distribution (DAD. The residues in the litter bags were dried, weighed, and ground, and then analyzed to quantify lignin, cellulose, non-structural biomass, total organic carbon (TOC, N, P, K, Ca, and Mg. In November 2012 and 2013, onion crops were harvested to quantify yield, and bulbs were classified according to diameter, and the number of rotted and flowering bulbs was determined. Soil in the 0.00-0.10 m layer was collected for chemical analysis before transplanting and after harvesting onion in December 2012 and 2013. The rye plant residues presented the highest half-life and they released less nutrients until 90 DAD. The great permanence of rye residue was considered a protection to soil surface, the opposite was observed with spontaneous vegetation. The cultivation and addition of dry residue of cover crops increased the onion yield at 2.5 Mg ha-1.

  19. Effects of fertilisation and irrigation practices on yield, maturity and storability of onions

    Directory of Open Access Journals (Sweden)

    T. SUOJALA

    2008-12-01

    Full Text Available The study aimed to establish whether a high onion yield and good storage performance could be obtained with low fertilisation rates if irrigation was applied when necessary. Two-year experiments investigated the effects of three NPK fertiliser levels (N 50, 100, 125/150 kg/ha, with and without irrigation, on yield, advancement of maturity, storage losses and shelf life. High fertilisation advanced maturity but irrigation had no effect. High fertilisation increased yield only in 1996 (5B7%, but irrigation increased the yield noticeably: by 33.5% in 1995 and 8.5% in 1996. There was no interaction between fertilisation and irrigation. The low fertilisation optimum is attributed to the mineralisation of soil nitrogen, as the soil was rich in organic matter. At the low fertilisation level, plants took up twice as much nitrogen as present in the fertiliser, and with increased fertilisation the nitrogen uptake increased markedly. The foliage nitrogen content was low, evidently as a result of late harvesting. Treatments had only a minor effect on the storage performance and shelf life of onions. The results suggest that fertilisation rates could be reduced in onion production. Irrigation during warm and dry periods is essential to achieve the maximum yield potential and does not impair the storage quality of onions.;

  20. Inoculation method, temperature and relative humidity affects leaf and neck anthracnose, a new onion disease.

    Science.gov (United States)

    Leaf and neck anthracnose caused by Colletotrichum coccodes is a new disease of onion in Michigan. To test the effect of inoculation method, ‘Prince’ onion seedlings were grown in the greenhouse and inoculated with either a conidial suspension of C. coccodes (alone or with an abrasive agent) or infe...

  1. Biochemical studies on some components of onion and garlic treated with gamma radiation

    International Nuclear Information System (INIS)

    Aly, A.N.E.

    2007-01-01

    the present work was carried out in order to evaluate the biological activities of garlic and onion essential oils as chemo preventive agents on male albino rats treated with AFB 1 -CCL 4 . the results showed that the main constituent of onion and garlic essential oil were dipropyl disulfide (DPDS) and diallyl trisulfide (DATS), respectively. the determination of different biological parameters showed a significant high levels of ALT,AST, caspase-3, GSH, total bilirubin, creatinine and urea in rat serum treated with AFB 1 -CCL 4 . on the other hand , a noticeable decrease in total protein, GST activity were detected, onion or garlic essential oils application have been found to regulate the biological parameters activities to the normal levels, besides, enhancing the accumulation of protein and their fractions. allicin compounds have been found to possess a lowering action of blood sugar

  2. Effects of pine pollen supplementation in an onion diet on Frankliniella fusca reproduction.

    Science.gov (United States)

    Angelella, G M; Riley, D G

    2010-04-01

    A micro-cage bioassay was developed to test the effect of slash pine pollen (Pinus elliottii Engelm.) supplementation to a whole onion plant (Allium cepa L. variety Pegasus) diet on thrips (Thysanoptera: Thripidae) reproductive parameters. Frankliniella fusca (Hinds) females were placed on two to three-leaf stage onion seedling under a treatment of either slash pine (Pinus elliottii Engelm.) pollen dusting (a pollen supplement) or no pollen treatment. Adult survival, net oviposition, and offspring produced over a series of ten 2-d intervals were recorded. From these values, l(x), l(x)m(x), and R(0) values were constructed. A trimodal distribution of oviposition was observed with the pollen supplement. Increased oviposition rates led to higher female offspring production per female and to a four-fold increase in F. fusca net reproduction on pollen-treated onions.

  3. Protective effect of onion extract against experimental ...

    African Journals Online (AJOL)

    Background: The wrong use of drugs results in disturbances in the immunity that affect human health. These drugs have side effects that may lead to death because of lake of immunity. Human beings need to use natural products to strength the immune system and avoid such side effects. Of these products is the onion that ...

  4. THE MAIN OUTCOME OF THE RUSSIAN\\BELARUSIAN COOPERATION IN BREEDING OF LEGUMES AND ONION CROPS

    Directory of Open Access Journals (Sweden)

    L. P. Shimansky

    2014-01-01

    Full Text Available The Russian-Belarusian cooperation in breeding of legumes and onion crops has resulted in development of new cultivars of pea (Samorodok, bean (Phaeton and Mignon, onion (Palesskaya znahodka, Patrida and Vermeles, winter garlic (Dubkovsky Asilak, which were included in 2014 in the State Register of the Republic of Belarus.

  5. POTASSIUM AND ITS EFFECT ON THE CONTENT OF POLYPHENOLS IN ONION (ALLIUM CEPA L.

    Directory of Open Access Journals (Sweden)

    Petra Kavalcová

    2015-02-01

    Full Text Available Onion (Allium cepa L. is rich of chemoprotective compounds as polyphenols, flavonoids, anthocyanins, vitamins, sulphur compounds which have potential beneficial properties for human health. Potassium as important mineral abundant plays many vital roles in plant nutrition (reduces respiration, activates enzyme. In generally, potassium increases crop yield and improves quality of onion bulbs. The objectives of this work were to compare and evaluate the impact of potassium on the content of total polyphenols and antioxidant activity of onion (Allium cepa L.. The content of the total polyphenols was determined by using the Folin-Ciocalteu reagent (FCR. The absorbance was measured at 765 nm of wave length against blank. Antioxidant activity was measured using a compound DPPH˙ (2.2-diphenyl-1-picrylhydrazyl at 515.6 nm in the spectrophotometer. The content of total polyphenols in samples of onion during vegatation period moved in the range from 505.6 mg GAE/kg ±25.18 to 621.49 mgGAE/kg ±13.41. In this work was watched also the influence of potassium on antioxidant activity, where values were in interval from 32.20 %± 0.58 to 44.67 % ±0.68.

  6. Influence of hydrogel on germination of lettuce and onion seed at different moisture levels

    Directory of Open Access Journals (Sweden)

    Kateřina Pazderů

    2013-01-01

    Full Text Available The influence of Agrisorb (water solution 1, 3, 5 g/l on lettuce and onion seed germination was tested in different moisture conditions (30 ml and 15 ml of water in germination box. Variants with reduced water level germinated much more slowly (MGT parameter than standard variants, though differences in total germination at the end of the test were insignificant. Treated variants of lettuce seeds showed a statistically significant increase in germination energy (GE on the first day (GE1, both water levels, but a significant decrease on the second day (columns GE2, 15 ml. Higher doses of Agrisorb slowed lettuce seed germination (GE2, 30 ml, dose 5 g significantly, similarly see GE2 (15 ml, doses 1, 3, 5 g. This slowdown was apparent for GE3 (both water amount as well. A similar but insignificant effect was evident for onions. There was an influence of cultivar and seed vigour on sensitivity to water stress. The hydrogel application influenced germination of lettuce and onion seeds. Treated lettuce seeds germinated faster than non-treated control in the beginning of germination process. This effect was not recorded in case of slowly germinated onion seed lots. Although influence of Agrisorb was positive in the beginning, higher doses of hydrogel reduced germination energy of treated seed lots (for example GE2, GE4 of both crops in comparison with non-treated control. Higher doses of hydrogel caused longer MGT of lettuce and onion as well.

  7. Molecular characterization of black Aspergillus species from onion and their potential for ochratoxin A and fumonisin B2 production.

    Science.gov (United States)

    Gherbawy, Youssuf; Elhariry, Hesham; Kocsubé, Sándor; Bahobial, Abdulaziz; Deeb, Bahig El; Altalhi, Abdulla; Varga, János; Vágvölgyi, Csaba

    2015-05-01

    Onion bulbs can become contaminated with various molds during the storage period, the most important causal agents being black aspergilli (Aspergillus section Nigri). Taxonomic studies have revealed that this group of Aspergillus contains many species that cannot be reliably identified using standard morphological methods. Therefore, it is necessary to define the fungus causing this problem in the onion exactly, especially since some species assigned to section Nigri are well known as ochratoxin and/or fumonisin producers. Sixty fungal isolates belonging to 10 fungal genera were isolated from 40 onion samples originated from the Taif region in Saudi Arabia. Black aspergilli were detected in 37 onion samples. Using primer pairs (awaspec and Cmd6) designed based on partial calmodulin gene sequence data, 37 isolates were identified as A. welwitschiae. The ochratoxin A and fumonisin B2 contents of the onion samples were examined. No ochratoxins were detected in the collected samples, while fumonisin B2 was detected in 37.5% of the onion samples. Eighteen of 37 isolates of Aspergillus welwitschiae were recognized as potential producers for fumonisin B2. Multiplex polymerase chain reactions designed to detect biosynthetic genes of fumonisins confirmed these results.

  8. Inoculum density of Glomus mosseae and growth of onion plants in unsterilized bituminous coal spoil

    Energy Technology Data Exchange (ETDEWEB)

    Khan, A.G.

    1988-01-01

    The effect of inoculum, density (number of vesicular-arbuscular mycorrhizal (VAM) propagules g/sup -1/ of inoculum) on the growth of onions (Allium cepa L.) infected by Glomus mosseae (Nicol and Gerd). Gerdemann and Trappe, Comb nov in unsterilized coal spoil containing indigenous VAM fungi, including G. mosseae, was investigated. The amount of onion roots converted to mycorrhizas by inoculant fungus, estimated by a gridline intersect method, increased with inoculum density (..gamma..0.62, P0.05) until a plateau was reached. Onion growth responses also increased significantly (P<0.05) with the amount of VAM inoculum present in the coal spoil. The initial linear relationship between inoculum propagules (MPN estimates), percent colonization of onion roots and onion shoot dry weight became quadratic as the number of infection propagules increased. VAM infection had no significant effect on root:shoot ratios. Similarly there was no significant interaction (P>0.05) between the inoculum density, VAM-colonized root mass and the onion root:shoot fresh weight ratios. The amount of the windswept bituminous coal spoil bound to VAM (presumably because of VAM external hyphae) also increased as inoculum density increased (..gamma..0.63, P<0.05). There was a stronger correlation (ga0.85, P<0.05) between the amount of spoil adhered per plant and the root fresh wt plant/sup -1/ indicating that root effects were primarily responsible for increasing spoil adherence. There were negative correlations (P<0.05) between root fresh wt plant/sup -1/ (..gamma..-0.68), inoculum density (..gamma..-0.589), percent root elngth infected (..gamma..-0.73) and the amount of spoil adhered g/sup -1/ root fresh wt. The possible exploitation of VAM in revegetation of bituminous coal spoil is discussed. 25 refs., 2 tabs.

  9. Economic feasibility studies on radiation preservation of dried and cured fishery products, onions and potatoes

    International Nuclear Information System (INIS)

    Ahmed, M.; Karim, A.; Quaiyum, M.A.; Bhuiya, A.D.; Matin, M.A.; Siddiqui, A.K.; Hossain, M.M.

    1989-01-01

    Dried and cured fishery products, onions and potatoes face enormous storage losses in Bangladesh due to insect infestation and sprouting. Research and development work was carried out to assess the suitability of introducing irradiation processing of these products in the country. Experiments showed that a dose of 0.04-0.68 kGy could inhibit sprouting in onions. Sprouting in potatoes could be inhibited at 0.10 kGy. Dried and cured fishery products could be disinfested of insects at a dose of 0.30 kGy. Infrastructure such as transportation, storage, marketing and existing systems of post-harvest handling were analysed. Post-harvest storage losses of onion and dried fish were more than 50% after 6 months of storage. Potatoes could not be kept at ambient conditions for over 3 months after the harvesting season. Irradiation of onions and dried fish, if they were stored in suitable conditions after proper packaging, could save significant storage losses. Irradiated potatoes could be stored at 14 deg. C instead of 2-4 deg. C as practised normally. On the basis of the data collected on dried and cured fishery products, onions and potatoes, economic feasibility studies were conducted. Assumptions for calculation of cost of the irradiation facility were: (i) strength of the irradiator source: 7.40 PBq of 60 Co; (ii) construction period: 2 years; (iii) operating time: 7200 hours per year; (iv) economic life: 20 years; (v) capacity utilization: 80-90%. In addition to dried and cured fish, potatoes and onions, this facility would also treat fresh fish and medical products in order to maximize its use. It would have an investment cost of US $1.9 million. The payback period was found to be less than 4 years. If additional warehouses could be built along with the facility, such a venture would be more profitable. 23 refs, 8 figs, 28 tabs

  10. Larger-scale irradiation and marketing studies on onions. Part of a coordinated programme on pre-commercial scale radiation treatment of food

    International Nuclear Information System (INIS)

    Kalman, B.

    1982-08-01

    Seed grown onions of ''Alsogodi'' variety were harvested manually, partially defoliated, irradiated with a dose of 50 Gy at a throughput of 6 tons per hour in a pilot-irradiator specially designed for onions, and then stored at ambient conditions of temperature and humidity, together with non-irradiated onions. Texture, density, growth of inner bud, colour, storage losses and quality were determined. Results clearly demonstrated that irradiation for sprout inhibition has a favourable effect on maintaining good texture of onions during storage. By inhibiting the sprouting, no internal discolouration of the bud occurred during storage. This is important for the quality of fresh onions as well as for that of the final dried product, processed from fresh onions after various periods of storage. Due to sub-optimal initial quality of the onions and unsatisfactory control of storage conditions, total storage losses were too high, compared to the results of previous experiments. Yet, the beneficial effect of irradiation was demonstrated at the end of the considered storage period, since 55% of the irradiated and only 10% of the non-irradiated commodities were of acceptable marketing quality. Cost calculation based on 80% utilization of the pilot irradiator used in this study resulted in a treatment cost of 0.25 forint per kg, which is approximately 8% of the average purchasing price of onions in Hungary

  11. Silencing onion lachrymatory factor synthase causes a significant change in the sulfur secondary metabolite profile.

    Science.gov (United States)

    Eady, Colin C; Kamoi, Takahiro; Kato, Masahiro; Porter, Noel G; Davis, Sheree; Shaw, Martin; Kamoi, Akiko; Imai, Shinsuke

    2008-08-01

    Through a single genetic transformation in onion (Allium cepa), a crop recalcitrant to genetic transformation, we suppressed the lachrymatory factor synthase gene using RNA interference silencing in six plants. This reduced lachrymatory synthase activity by up to 1,544-fold, so that when wounded the onions produced significantly reduced levels of tear-inducing lachrymatory factor. We then confirmed, through a novel colorimetric assay, that this silencing had shifted the trans-S-1-propenyl-l-cysteine sulfoxide breakdown pathway so that more 1-propenyl sulfenic acid was converted into di-1-propenyl thiosulfinate. A consequence of this raised thiosulfinate level was a marked increase in the downstream production of a nonenzymatically produced zwiebelane isomer and other volatile sulfur compounds, di-1-propenyl disulfide and 2-mercapto-3,4-dimethyl-2,3-dihydrothiophene, which had previously been reported in trace amounts or had not been detected in onion. The consequences of this dramatic simultaneous down- and up-regulation of secondary sulfur products on the health and flavor attributes of the onion are discussed.

  12. Comparative Analysis of Two Flavonol Synthases from Different-Colored Onions Provides Insight into Flavonoid Biosynthesis.

    Science.gov (United States)

    Park, Sangkyu; Kim, Da-Hye; Lee, Jong-Yeol; Ha, Sun-Hwa; Lim, Sun-Hyung

    2017-07-05

    We isolated cDNAs encoding flavonol synthase (FLS) from the red onion "H6" (AcFLS-H6) and the yellow onion "Hwangryongball" (AcFLS-HRB). We found three amino acid variations between the two sequences. Kinetic analysis with recombinant proteins revealed that AcFLS-HRB exhibited approximately 2-fold higher catalytic efficiencies than AcFLS-H6 for dihydroflavonol substrates and that both proteins preferred dihydroquercetin to dihydrokaempferol. The expression patterns of flavonoid biosynthesis genes corresponded to the accumulation patterns of flavonoid aglycones in both onions. Whereas the other flavonoid biosynthesis genes were weakly expressed in the HRB sheath compared to that of H6, the expression of FLS was similar in both onions. This relatively enhanced FLS expression, along with the higher activity of AcFLS-HRB, could increase the quercetin production in the HRB sheath. The quercetin content was approximately 12-fold higher than the cyanidin content in the H6 sheath, suggesting that FLS has priority in the competition between FLS and dihydroflavonol 4-reductase (DFR) for their substrate dihydroquercetin.

  13. Garlic and onion sensitization among Saudi patients screened for ...

    African Journals Online (AJOL)

    EB

    2013-09-03

    Sep 3, 2013 ... consumed foods materials such as garlic and onion have rarely been investigated for ... among the members of Liliaceae family 6 but also .... correlate well with the severity of clinical signs and ... This being a ... adolescents.

  14. Effects of Digested Onion Extracts on Intestinal Gene Expression: An Interspecies Comparison Using Different Intestine Models.

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    Nicole J W de Wit

    Full Text Available Human intestinal tissue samples are barely accessible to study potential health benefits of nutritional compounds. Numbers of animals used in animal trials, however, need to be minimalized. Therefore, we explored the applicability of in vitro (human Caco-2 cells and ex vivo intestine models (rat precision cut intestine slices and the pig in-situ small intestinal segment perfusion (SISP technique to study the effect of food compounds. In vitro digested yellow (YOd and white onion extracts (WOd were used as model food compounds and transcriptomics was applied to obtain more insight into which extent mode of actions depend on the model. The three intestine models shared 9,140 genes which were used to compare the responses to digested onions between the models. Unsupervised clustering analysis showed that genes up- or down-regulated by WOd in human Caco-2 cells and rat intestine slices were similarly regulated by YOd, indicating comparable modes of action for the two onion species. Highly variable responses to onion were found in the pig SISP model. By focussing only on genes with significant differential expression, in combination with a fold change > 1.5, 15 genes showed similar onion-induced expression in human Caco-2 cells and rat intestine slices and 2 overlapping genes were found between the human Caco-2 and pig SISP model. Pathway analyses revealed that mainly processes related to oxidative stress, and especially the Keap1-Nrf2 pathway, were affected by onions in all three models. Our data fit with previous in vivo studies showing that the beneficial effects of onions are mostly linked to their antioxidant properties. Taken together, our data indicate that each of the in vitro and ex vivo intestine models used in this study, taking into account their limitations, can be used to determine modes of action of nutritional compounds and can thereby reduce the number of animals used in conventional nutritional intervention studies.

  15. Energy Efficiency Evaluation and Economical Analysis of Onion(Allium Cepa L. Production in Khorasan Razavi Province of Iran

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    F. Hassanzadeh Aval

    2013-07-01

    Full Text Available Current conventional agricultural systems using intensive energy have to be revitalized through new integrated approaches relying on renewable energy resources, which can allow farmers to stop dependence on fossil resources. The aim of this study was determining the amount of input–output energy used in onion (Allium cepa L. production in Khorasan Razavi province. For this purpose, the data was collected from 55 onion farm workers in Khorasan Razavi. Inquiries were conducted in face-to-face interviews in April-May 2011. Farm workers were selected based on random sampling method. The results indicated that total energy input was 98479 MJ.ha-1. The share of electricity and nitrogen fertilizer was 50.9 and 14.7%, respectively out of total energy input. The average onion yield under normal conditions was 73227 kg.ha-1 on irrigated farms. The net energy and energy productivity values were estimated to be 18684 MJ.ha-1 and 0.74 kg.MJ-1, respectively, and the ratio of energy output to energy input was found to be 1.19. This indicated an intensive use of input in onion production, which is not parallel to the increase in the final yield. Approximately 77.3% of the total energy input used in onion production was non-renewable (machinery, diesel, fertilizers, chemicals and electricity and only 22.7% was renewable energy form (human labor, manure, water for irrigation, seeds. Cost analysis revealed that total cost of onion production for one hectare was 79262908 Rials. Benefit–cost ratio was calculated as 1.65. Although energy efficiency of onion production in Khorasan Razavi province is low, it is economically justified.

  16. Marketable yield of onion under different irrigation depths, with and without mulch

    Directory of Open Access Journals (Sweden)

    Daniel F. de Carvalho

    Full Text Available ABSTRACT The objectives of this study were to obtain the onion crop coefficients and evaluate the influence of different irrigation depths (0, 22, 45, 75 and 100% of crop evapotranspiration on marketable yield and quality of onion bulbs cultivated with and without mulch of elephant grass. The experiment was carried out in Seropédica, RJ, Brazil, from May to September 2012, in a Red Yellow Argisol. The experimental design was in randomized blocks in split plots, with 10 treatments and seven replicates. Irrigation management was performed through soil water balance using the Time Domain Reflectometry technique, with probes installed horizontally at 7.5 and 22.5 cm depths. The use of mulch allowed the application of smaller irrigation depths, leading to lower crop coefficient (18% in stage II and 3% in stage III in comparison to the crop without mulch. Irrigation depths associated with the use of mulch influenced the evaluated production variables, proving to be an alternative to increase marketable yield and quality of onion bulbs, with lower irrigation depth.

  17. Phenotypic diversity of basic characteristics of genotypes from the Serbia onion collection

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    Gvozdanović-Varga Jelica

    2013-01-01

    Full Text Available The onion is a widely distributed vegetable crop, which takes an important place in the vegetable production in Serbia. The traditional planting method is the one from sets. Old cultivars and populations and, in recent years, foreign cultivars are grown. The large variety of genotypes, including both domestic populations and cultivars, comprises the significant gene pool of this region. The onion collection of the Institute of Field and Vegetable Crops in Novi Sad is based on the populations and cultivars from the territory of the former Yugoslavia. This paper reviews 30 onion genotypes on the basic IPGRI descriptors (ANNEX I. Variability of the reviewed characteristics was determined by PC analysis. High variability values have been established for bulb skin color, bulb flesh color, bulb hearting and bulb skin thickness. The genotypes varied in bulb skin color as well as in bulb flesh color from white to dark violet. These two characteristics had the largest impact on clustering, with a single genotype being heterogeneous exactly for these two characteristics. [Projekat Ministarstva nauke Republike Srbije, br. TR 31030

  18. Changes in endogenous gibberellin-like substances in onion bulbs (Allium cepa L. cv. Sochaczewska during storage

    Directory of Open Access Journals (Sweden)

    Elżbieta Kielak

    2013-12-01

    Full Text Available Onions cv. Sochaczewska were dried up under an umbrella roof till October 15th or till November 15th and thereafter stored in a cold room at 0-1°C until May 15th. During 4-year of experiment gibberellin activity was determined each month during storage. Three periods of high activity of gibberellins were found in most cases: in December, in February-March and in late spring. In general, onions dried longer (till November 15th, showed lower gibberellin activity than onions dried shorter. Gibberellin-like substances under investigation are localized in almost all Rf zones of chromatogram. Sprouting and rooting was related to gibberellin activity.

  19. Semen quality characteristics of Koekoek breeder cocks influenced by supplemental inclusion levels of onion and garlic mixture at 35-41 weeks of age

    Directory of Open Access Journals (Sweden)

    Victor Mela Obinna Okoro

    Full Text Available ABSTRACT An experiment was conducted to determine the effect of varying dietary supplemental inclusion levels of onion and garlic mixture on semen quality characteristics of Koekoek breeder cocks aged 35-41 weeks. The experimental diets were isocaloric and isonitrogenous but with different supplemental inclusion levels of onion and garlic. A complete randomized design was used for the experiment. The three dietary supplemental levels based on garlic and onion supplemental inclusion levels were Q0 (0 g onion and 0 g garlic per 600 g DM feed, Q1 (2.5 g onion and 2.5 g garlic per 600 g DM feed, and Q2 (5 g onion and 5 g garlic per 600 g DM feed with each treatment having three replicates. A quadratic type equation was used to determine the onion and garlic levels for optimum semen quality characteristics. Daily supplementation with 5 g onion and 5 g garlic per 600 g DM feed increased progressive motile cells (%, actual life sperm count (×106/nl, and live sperm (% by 221.20%, 301.51%, and 352.43%, while 2.5 g onion and 2.5 g garlic per 600 g DM feed reduced them by 28.67%, 12.69%, and 19.00%, respectively. However, daily supplementation with 2.5 g onion and 2.5 g garlic per 600 g DM feed increased sperm count (×106/nl by 12.82%, whereas daily supplementation with 5 g onion and 5 g garlic per 600 g DM feed reduced it by 10.26% in Koekoek breeder cocks. Dietary onion and garlic supplemental inclusion levels of 3.009, 3.191, 4.621, 6.601, 6.719, 2.327, 2.385, and 2.247 g per 600 g DM feed supported optimum progressive motile cells (%, immotile sperm cells (%, actual dead sperm count (×106/nl, actual live sperm count (×106/nl, live sperm (%, acrosome morphology defects (%, acrosome detachment (%, and acrosome swelling (%, with probability values ranging from 0.003 to 0.783, whereas minimum progressive motile cells (% increase was achieved at an optimum onion and garlic supplementation levels of 3.009 g per 600 g DM feed. These findings have a lot of

  20. Fusarium rot of onion and possible use of bioproduct

    Directory of Open Access Journals (Sweden)

    Klokočar-Šmit Zlata

    2008-01-01

    Full Text Available Several species of Fusarium are causal agents of onion rot in field and storage. Most prevalent are F. oxysporum f. sp. cepae and F. solani, and recently F. proliferatum, a toxigenic species. Most frequently isolated fungi in our field experiments were F. solani and F. proliferatum with different pathogenicity. Certain differences in antagonistic activity of Trichoderma asperellum on different isolates of F. proliferatum and F. solani have been found in in vitro study in dual culture, expressed as a slower inhibition of growth of the former, and faster of the latter pathogen. Antagonistic abilities of species from genus Trichoderma (T. asperellum are important, and have already been exploited in formulated biocontrol products in organic and conventional production, in order to prevent soil borne pathogens inducing fusarium wilt and rot. The importance of preventing onion infection by Fusarium spp., possible mycotoxin producers, has been underlined.

  1. Storage properties of irradiated potatoes and onions. Part of a coordinated programme on the Asian Regional Cooperative Project on Food Irradiation

    International Nuclear Information System (INIS)

    Hayashi, T.

    1984-12-01

    Quality changes with regard to browning of inner buds and sprouting of irradiated onions variety Kitamiki and Ohotsuku were investigated. One ton of each variety was irradiated with either 38.1 or 65.2 Gy at the Commercial Potato Irradiator in Shihoro and stored either at 0 deg. C or 5 deg. C. After 6 months of storage, they were taken out to be stored at room temperature. It was observed that green inner buds developed in most unirradiated onions which showed no external sprouting irrespective of storage temperature. Irradiated onions show no external or internal sprouting but exhibited browning of small inner buds. Unirradiated onions rooted slightly during storage at low temperature. None of the irradiated samples showed rooting. Neither of the samples spoiled significantly during storage. It was concluded that storage at room temperature accelerated sprouting and development of green inner buds of unirradiated onions and browning of small inner buds in irradiated ones. During off-seasons, only irradiated onions stored at low temperatures have qualities of commercial significance

  2. Field dissipation of oxyfluorfen in onion and its dynamics in soil under Indian tropical conditions.

    Science.gov (United States)

    Janaki, P; Sathya Priya, R; Chinnusamy, C

    2013-01-01

    Oxyfluorfen, a diphenyl-ether herbicide is being used to control annual and perennial broad-leaved weeds and sedges in a variety of field crops including onion. The present study was aimed to investigate the dynamics and field persistence of oxyfluorfen in onion plant, bulb and soil under Indian tropical conditions. Application of four rates of oxyfluorfen viz., 200, 250, 300 and 400 g AI ha(-1) as pre-emergence gave good weed control in field experiment with onion. The oxyfluorfen residue dissipated faster in plant than in soil respectively, with a mean half-life of 6.1 and 11.2 days. Dissipation followed first-order kinetics. In laboratory column leaching experiments, 17 percent of the applied oxyfluorfen was recovered from the soil and indicates its solubility in water and mobility in sandy clay loam soil was low. A sorption study revealed that the adsorption of oxyfluorfen to the soil was highly influenced by the soil organic carbon with the Koc value of 5450. The study concludes that the dissipation of oxyfluorfen in soil and onion was dependent on the physico-chemical properties of the soil and environmental conditions.

  3. Co-fermentation of onion and whey: A promising synbiotic combination

    DEFF Research Database (Denmark)

    Tinello, Federica; Vendramin, Veronica; Barros Divino, Vitoria

    2017-01-01

    Juice from three different onion varieties was mixed with sweet whey and used as growth substrate for four lactic acid bacteria strains, isolated from agri-food by-products, to evaluate the possibility to exploit such substrates, known to be reach in bioactive molecules, as fermented drinks...... for human consumption. Results show good growth performance for Lactobacillus fabifermentans, L. plantarum and Streptococcus macedonicus. On the contrary S. thermophilus did not grow in the mixture while S. macedonicus did not develop in pure onion juice. After 48 h the overall sugar content decreased...... significantly. In particular, glucose was not utilized while inulin was completely preserved. Moreover, MS/MS analysis revealed the presence of the rare trisaccharide lactosucrose. In the light of these considerations, the formulation obtained may be considered a potential synbiotic product with pleasant taste...

  4. concentration levels of major and trace metals in onion

    African Journals Online (AJOL)

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    like stews, soups, tomato sauces, cooked vegetables and meat by slicing, chopping, ... including metal concentrations in soils, soil pH, cation exchange capacity, ... determination was done by flame atomic absorption spectrophotometer (FAAS) (Buck ...... Margen, S. Onions, The Wellness Encyclopedia of Food and Nutrition, ...

  5. Dissection of Trichoderma longibrachiatum-induced defense in onion (Allium cepa L.) against Fusarium oxysporum f. sp. cepa by target metabolite profiling.

    Science.gov (United States)

    Abdelrahman, Mostafa; Abdel-Motaal, Fatma; El-Sayed, Magdi; Jogaiah, Sudisha; Shigyo, Masayoshi; Ito, Shin-Ichi; Tran, Lam-Son Phan

    2016-05-01

    Trichoderma spp. are versatile opportunistic plant symbionts that can cause substantial changes in the metabolism of host plants, thereby increasing plant growth and activating plant defense to various diseases. Target metabolite profiling approach was selected to demonstrate that Trichoderma longibrachiatum isolated from desert soil can confer beneficial agronomic traits to onion and induce defense mechanism against Fusarium oxysporum f. sp. cepa (FOC), through triggering a number of primary and secondary metabolite pathways. Onion seeds primed with Trichoderma T1 strain displayed early seedling emergence and enhanced growth compared with Trichoderma T2-treatment and untreated control. Therefore, T1 was selected for further investigations under greenhouse conditions, which revealed remarkable improvement in the onion bulb growth parameters and resistance against FOC. The metabolite platform of T1-primed onion (T1) and T1-primed onion challenged with FOC (T1+FOC) displayed significant accumulation of 25 abiotic and biotic stress-responsive metabolites, representing carbohydrate, phenylpropanoid and sulfur assimilation metabolic pathways. In addition, T1- and T1+FOC-treated onion plants showed discrete antioxidant capacity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) compared with control. Our findings demonstrated the contribution of T. longibrachiatum to the accumulation of key metabolites, which subsequently leads to the improvement of onion growth, as well as its resistance to oxidative stress and FOC. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  6. Development of Biocomposites with Antioxidant Activity Based on Red Onion Extract and Acetate Cellulose

    Directory of Open Access Journals (Sweden)

    Carol López de Dicastillo

    2015-08-01

    Full Text Available Antioxidant biocomposites have been successfully developed from cellulose acetate, eco-friendly triethyl citrate plasticizer and onion extract as a source of natural antioxidants. First, an onion extraction process was optimized to obtain the extract with highest antioxidant power. Extracts under absolute ethanol and ethanol 85% were the extracts with the highest antioxidant activity, which were the characterized through different methods, DPPH (2,2-diphenyl-1-picrylhydrazyl and ABTS (2,2ʹ-azinobis(3-ethylbenzothiazoline-6-sulphonate, that measure radical scavenger activity, and polyphenolic and flavonoid content. Afterwards, the extract was incorporated in cellulose acetate as polymer matrix owing to develop an active material intended to oxidative sensitive food products packaging. Different concentrations of onion extract and plasticizer were statistically studied by using response surface methodology in order to analyze the influence of both factors on the release of active compounds and therefore the antioxidant activity of these materials.

  7. Investigations on the possible genotoxic effect of irradiated onion powder by means of prophage induction (inductest)

    Energy Technology Data Exchange (ETDEWEB)

    Farkas, J; Andrassy, E [Koezponti Elelmiszeripari Kutato Intezet, Budapest (Hungary)

    1982-01-01

    Aqueous extracts and enzymic digests of onion powder, untreated, irradiated with 5.0 and 10.0 kGy gamma radiation doses under aerobic conditions were tested with lysogenic strains GY 5022 and GY 5027 of Escherichia coli K12, for their prophage lambda inducing effect in the course of the genotoxicologic study carried out as a part of the wholesomeness testing of irradiated spices and condiments in the frame of the International Project in the Field of Food Irradiation (IFIP). The tests were carried out on onion powder stored for 3 months subsequent to radiation treatment. One ..mu..g of aflatoxin B/sub 1/ or 1 ..mu..g of streptozotocin per test was used as a positive control. The amounts of extracts and enzymic digests exposed to prophage induction test corresponded to about 55 mg and 22 mg, resp., of onion powder. While both aflatoxin and streptozotocin, known for their carcinogenic and mutagenic effect, exerted prophage induction, no statistically significant difference was observed between the frequency of spontaneous phage induction and that occurring in the presence of either untreated or irradiated onion powder. In tests carried out with thermally inducible lambdac 1857 test organisms (Escherichia coli K12 GY 5024 and GY 5029) onion powder, used in amounts as indicated above, did not damage either the prophage or the host organism in a way to affect the induced prophage propagating capacity of the cells.

  8. Investigations on the possible genotoxic effect of irradiated onion powder by means of prophage induction (inductest)

    International Nuclear Information System (INIS)

    Farkas, J.; Andrassy, E.

    1982-01-01

    Aqueous extracts and enzymic digests of onion powder, untreated, irradiated with 5.0 and 10.0 kGy gamma radiation doses under aerobic conditions were tested with lysogenic strains GY 5022 and GY 5027 of Escherichia coli K12, for their prophage lambda inducing effect in the course of the genotoxicologic study carried out as a part of the wholesomeness testing of irradiated spices and condiments in the frame of the International Project in the Field of Food Irradiation (IFIP). The tests were carried out on onion powder stored for 3 months subsequent to radiation treatment. One μg of aflatoxin B 1 or 1 μg of streptozotocin per test was used as a positive control. The amounts of extracts and enzymic digests exposed to prophage induction test corresponded to about 55 mg and 22 mg, resp., of onion powder. While both aflatoxin and streptozotocin, known for their carcinogenic and mutagenic effect, exerted prophage induction, no statistically significant difference was observed between the frequency of spontaneous phage induction and that occurring in the presence of either untreated or irradiated onion powder. In tests carried out with thermally inducible lambdac 1857 test organisms (Escherichia coli K12 GY 5024 and GY 5029) onion powder, used in amounts as indicated above, did not damage either the prophage or the host organism in a way to affect the induced prophage propagating capacity of the cells. (author)

  9. Proteomic Analysis of Male-Fertility Restoration in CMS Onion

    Science.gov (United States)

    The production of hybrid-onion seed is dependent on cytoplasmic-genic male sterility (CMS) systems. For the most commonly used CMS, male-sterile (S) cytoplasm interacts with a dominant allele at one nuclear male-fertility restoration locus (Ms) to condition male fertility. We are using proteomics ...

  10. Protective role of onion and garlic on physicochemical alterations ...

    African Journals Online (AJOL)

    SERVER

    2007-09-19

    Sep 19, 2007 ... (Alexander, 1977, 1978; Miller and Long, 1990; Sanders,. 1989). ... ley Market, Freetown, Sierra Leone. They were cut into ... was fed with heated oil + 0.2 g onion/ml, and D was fed with heated oil + 0.2 g .... 231: 43-54. Cao G ...

  11. In vitro antibacterial activity of onion (allium cepa) against clinical isolates of vibrio cholera

    International Nuclear Information System (INIS)

    Hannan, A.; Humayun, T.; Hussain, M.B.; Yasir, M.; Sikandar, S.

    2010-01-01

    Background: Cholera is a major public health problem in developing countries of the world. Bacterial resistance, lack of surveillance data and proper microbiological facilities are major problems regarding diagnosis of cholera. The spread of microbial drug resistance is a global public health challenge that results in increased illness and death rate. Newer antimicrobials or agents are urgently required to overcome this problem. This work was therefore done to investigate the antimicrobial potential of onion against thirty-three clinical isolates of Vibrio cholera. Methods: The extract was prepared by reflux extraction method. Antibacterial screening of clinical isolates of V. cholerae was done by agar well diffusion method. Agar dilution method was used to assess the Minimum Inhibitory Concentration (MIC). Results: All tested strains of V. cholerae were sensitive to onion (Allium cepa) extracts of two types (purple and yellow). Purple type of extract had MIC range of 19.2-21.6 mg/ml. The extract of yellow type onion had an MIC range of 66-68.4 mg/ml. Conclusion: The results indicated that onion (Allium cepa) has an inhibitory effect on V. cholerae. Keeping in view the anti-bacterial activity of this compound can be exploited as a therapeutic agent in an animal model. This finding is a positive point for further investigation of this herb of traditional medicine. (author)

  12. Preliminary evaluation of maggot (Diptera: Calliphoridae therapy as a potential treatment for leishmaniasis ulcers Evaluación preliminar en un modelo animal de la terapia con larvas de Lucilia sericata para el tratamiento de la leishmaniasis cutánea

    Directory of Open Access Journals (Sweden)

    Jazzmin Arrivillaga

    2008-06-01

    Full Text Available Introduction. Maggot debridement therapy has been widely used for treating a variety of scarred-over soft-tissue wounds. Published accounts record several illnesses in which treatment with larval therapy has promoted injury healing in conjunction with infection by bacterial pathogens resistant to conventional antibiotics.
    Objective. An initial test of the maggot therapy was developed for cutaneous injuries produced by Leishmania amazonensis.
    Materials and methods. An experimental design based on an animal model with three replicates in Mesocricetus aureatus (Rodentia: Muridae was used to evaluate size variation lesion before and after after larval therapy with Lucilia sericata maggots. The criteria used for therapy evaluation were lesion size, maggot application time, and presence or absence of edema and secretions.
    Results.  Effective scarring and wound healing was observed after therapy with L. sericata larvae, i.e. 80% to 100% lesion area reduction after 12 hours.
    Conclusion. The preliminary results suggest that fly maggots of L. sericata have a potential use as natural medical and veterinary alternative therapy for the cutaneous leishmaniasis.

    Introducción. La terapia con larvas ha sido ampliamente utilizada para el tratamiento de lesiones ulcerativas de la piel; existen registros de enfermedades, como podopatía diabética, osteomielitis y úlceras varicosas, en las cuales el uso de la terapia con larvas ha promovido la cicatrización de la lesión en presencia de patógenos bacterianos resistentes a los antibióticos convencionales.
    Objetivo. Realizar una prueba piloto de terapia con larvas de Lucilia sericata sobre lesiones cutáneas producidas por Leishmania amazonensis.
    Materiales y métodos. En el presente trabajo se empleó un diseño experimental en animales ( Mesocricetus aureatus, tres réplicas con la finalidad de analizar las variaciones del tamaño de la lesión por leishmaniasis antes y

  13. Microbiological Aspects Considering the Production of Nutraceutical Curd Containing Onion

    Directory of Open Access Journals (Sweden)

    Anca Dumuţa

    2016-10-01

    Full Text Available The curd is a traditional Romanian dairy product highly appreciated by the consumers. The purpose of this study was to produce and analyze from the microbiological point of view a nutraceutical curd containing onion, as a prebiotic source. The synbiotics generate among the organoleptic benefits also health improvement due to the different actions taken by probiotics and prebiotics, working together. The curd was produced by the traditional method using buffalo milk with reduced cholesterol content due to the treatment with crosslinked β-ciclodextrin with adipic acid.The curd prepared by rennet adding and coagulation at 30°C during 30-45 minutes was subjected to the maturation at a temperature of 12-16°C. Considering the microbiological tests, the curd recorded a pozitive evolution, being characterized by a lower microbial load compared with a control curd, prepared without onion.

  14. Preservation of potatoes and onions by irradiation and chemical treatments. A status report

    International Nuclear Information System (INIS)

    Sekhavat, A.; Zare, Z.; Kudva, M.G.; Chopra, K.L.; Shariat Panahi, J.M.

    1978-01-01

    Preservation of potatoes and onions is an urgent need in Iran, and an attempt has been made to gain experience in this field by applying ionizing radiation and chemicals. 60 Co gamma radiation doses of 5, 10, 12 and 15krad for three local varieties of potatoes and of 3, 6 and 9krad for one variety of onions, salicylic acid concentrations of 500, 1500 and 2500ppm, and combinations of different doses of radiation and salicylic acid were tried. Upon irradiation with doses of 12-15krad a significant reduction in the losses due to sprouting, rotting and dehydration was observed for all three varieties of potatoes after a storage period of nine months. Chemical treatment alone proved ineffective. Some doses of a combined treatment with salicylic acid plus irradiation yielded good results, but they need confirmation by further investigation. Organoleptic tests were carried out on two varieties of potatoes. Due to a delay in starting the treatment of onions after harvest, a high percentage had already begun to sprout and so the results obtained are only approximate values. (author)

  15. Preservation of Potatoes and Onions by Irradiation and Chemical Treatments. A Status Report

    Energy Technology Data Exchange (ETDEWEB)

    Sekhavat, A.; Zare, Z.; Kudva, M. G.; Chopra, K. L.; Shariat Panahi, J. M. [Center for Research and for Application Of Radioisotopes, Atomic Energy Organization of Iran, Tehran (Iran, Islamic Republic of)

    1978-04-15

    Preservation of potatoes and onions is an urgent need in Iran, and an attempt has been made to gain experience in this field by applying ionizing radiation and chemicals. {sup 60}Co gamma radiation doses of 5, 10, 12 and 15 krad for three local varieties of potatoes and of 3, 6 and 9 krad for one variety of onions, salicylic acid concentrations of 500, 1500 and 2500 ppm, and combinations of different doses of radiation and salicylic acid were tried. Upon irradiation with doses of 12-15 krad a significant reduction in the losses due to sprouting, rotting and dehydration was observed for all three varieties of potatoes after a storage period of nine months. Chemical treatment alone proved ineffective. Some doses of a combined treatment with salicylic acid plus irradiation yielded good results, but they need confirmation by further investigation. Organoleptic tests were carried out on two varieties of potatoes. Due to a delay in starting the treatment of onions after harvest, a high percentage had already begun to sprout and so the results obtained are only approximate values. (author)

  16. Chemical Composition and Antioxidant Properties of Five White Onion (Allium cepa L. Landraces

    Directory of Open Access Journals (Sweden)

    Loredana Liguori

    2017-01-01

    Full Text Available Five onion landraces belonging to Bianca di Pompei cv., cultivated in Campania region (Italy, were characterized for their main quality parameters. The onion landraces were harvested at the end of the growth cycle corresponding to the ripening time and harvest month, respectively: February, March, April, May, and June. The total content of volatile compounds as well as the sulfur-containing compounds in Aprilatica was significantly (p≤0.05 higher than the other landraces investigated. The nutraceutical feature investigated through the total phenols, phenols profile, and antioxidant activity showed higher values for the samples harvested in spring months. High pungency values ranging from 9 to 14 μmol/g FW were found in all onion landraces investigated as enzymatically (alliinase produced pyruvate (EPY. The organic acids profile (malic, citric, succinic, pyruvic, oxalic, ascorbic, and tartaric acids highlighted malic and citric acids in higher amounts in all landraces. Fructose, glucose, and sucrose were found as soluble sugars and fructose was the most abundant. Generally, the results highlighted the growth temperature influence on the investigated quality parameters.

  17. Multielement fingerprinting as a tool in origin authentication of PGI food products: Tropea red onion.

    Science.gov (United States)

    Furia, Emilia; Naccarato, Attilio; Sindona, Giovanni; Stabile, Gaetano; Tagarelli, Antonio

    2011-08-10

    Tropea red onion ( Allium cepa L. var. Tropea) is among the most highly appreciated Italian products. It is cultivated in specific areas of Calabria and, due to its characteristics, was recently awarded with the protected geographical indications (PGI) certification from the European Union. A reliable classification of onion samples in groups corresponding to "Tropea" and "non-Tropea" categories is now available to the producers. This important goal has been achieved through the evaluation of three supervised chemometric approaches. Onion samples with PGI brand (120) and onion samples not cultivated following the production regulations (80) were digested by a closed-vessel microwave oven system. ICP-MS equipped with a dynamic reaction cell was used to determine the concentrations of 25 elements (Al, Ba, Ca, Cd, Ce, Cr, Dy, Eu, Fe, Ga, Gd, Ho, La, Mg, Mn, Na, Nd, Ni, Pr, Rb, Sm, Sr, Tl, Y, and Zn). The multielement fingerprint was processed using linear discriminant analysis (LDA) (standard and stepwise), soft independent modeling of class analogy (SIMCA), and back-propagation artificial neural network (BP-ANN). The cross-validation procedure has shown good results in terms of the prediction ability for all of the chemometric models: standard LDA, 94.0%; stepwise LDA, 94.5%; SIMCA, 95.5%; and BP-ANN, 91.5%.

  18. Onion and garlic extracts as potential antidotes for cadmium-induced biochemical alterations in prostate glands of rats.

    Science.gov (United States)

    Ola-Mudathir, F K; Suru, S M

    2015-11-01

    Cadmium (Cd) has been implicated in increased prostate gland malignancy risk in both wildlife and humans. This study examines the chemoprotective roles of onion and garlic extracts on Cd-induced biochemical alterations in the prostate glands of rats. Adult male Wistar rats were randomly divided into nine groups: control group received double distilled water; Cd group received Cd alone (1.5 mg/100 g bwt per day); extract-treated groups were pre-treated with varied doses of onion and/or garlic extract (0.5 ml and 1.0 ml/100 g bwt per day) for 1 week and then co-treated with Cd (1.5 mg/100 g bwt per day) for additional 3 weeks. Oxidant/antioxidant status and acid phosphatase (ACPtotal and ACPprostatic ) activity were examined in prostate glands. Cd intoxication caused a marked (P garlic extract significantly minimised these alterations. The onion extract offered a dose-dependent protection. Our findings suggest a chemoprotective capability for onion and garlic extracts against Cd-induced biochemical alteration in the prostate glands. © 2014 Blackwell Verlag GmbH.

  19. [Effects of intercropping Chinese onion cultivars of different allelopathic potential on cucumber growth and soil micro-environment].

    Science.gov (United States)

    Yang, Yang; Wu, Feng-zhi

    2011-10-01

    A pot experiment was conducted to study the effects of intercropping various Chinese onion cultivars of different allelopathic potential on the cucumber growth and rhizospheric soil environment. When intercropped with high allelopathic Chinese onion cultivars, the EC value and peroxidase activity of cucumber rhizospheric soil decreased, while the pH value, invertase and catalase activities, and bacterial community diversity increased. The cloning and sequencing results indicated that most DGGE bands amplified from cucumber rhizospheric soil samples showed a high homology to uncultured bacterial species. The common bands were affiliated with Actinobacteria and Proteobacteria, and the differential bacteria bands were affiliated with Proteobacteria and Anaerolineaceae. Rhodospirillales and Acidobacteria were only found in the cucumber rhizospheric soil intercropped with low allelopathic Chinese onion cultivars. Correlation analysis showed that there were significant positive correlations between rhizospheric soil urease activity and cucumber seedlings height, total dry biomass, leaf area, and DGGE band number. It was suggested that intercropping high allelopathic Chinese onion cultivars could establish a good rhizospheric soil micro-environment for cucumber growth, and promote the growth of cucumber seedlings markedly.

  20. Evaluation of polyphenolic content and antioxidant activity in two onion varieties grown under organic and conventional production systems.

    Science.gov (United States)

    Ren, Feiyue; Reilly, Kim; Gaffney, Michael; Kerry, Joseph P; Hossain, Mohammad; Rai, Dilip K

    2017-07-01

    Onions contain a number of bioactive compounds, in particular polyphenols. They are rich sources of such compounds in the human diet and offer significant health benefits to the consumer. Demand for organic crops is steadily increasing partly based on the expected health benefits of organic food consumption. The current study examines the influence of organic and conventional crop management practices on bioactive polyphenolic content of onion. We examined the effect of conventional, organic, and mixed cultivation practices on the content of total phenolics, total flavonoids and antioxidant activity in two varieties of onion grown over 4 years in a split-plot factorial systems comparison trial. Levels of total phenolics and total flavonoids showed a significant year-on-year variation and were significantly different between organic and conventional production systems. The levels of total phenolics, total flavonoids and antioxidant activity in general were significantly higher (P onion. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  1. Irradiation effect on onion keeping-quality after sea-shipment from Argentina to the Netherlands

    International Nuclear Information System (INIS)

    Wolters, T.C.; Langerak, D.I.; Curzio, O.A.; Croci, C.A.

    1990-01-01

    Onion losses occur due to sprouting and rot. Onion cultivar Valenciana was irradiated with 60 Gy, shipped from Argentina to the Netherlands, and stored 6 months at 4 degrees C. The rot in unirradiated samples increased after 4 months while rot in irradiated samples remained almost constant. Irradiation almost completely prevented sprouting. Storage losses were 50% for unirradiated and 20% for irradiated samples. Irradiated samples showed an improvement in general appearance. Irradiation reduced weight loss and damaged bulbs were more susceptible to storage rot

  2. Economic feasibility study of onion and potato irradiation in the Syrian Arab Republic

    International Nuclear Information System (INIS)

    Al-Bachir, M.; El-Den-Sharabi, N.; Midani, M.Ayman

    1995-01-01

    The purpose of this study was to examine the economic and financial feasibility of a food irradiation project proposed by the Syrian Atomic Energy Commission. The prime purpose of the project was to irradiate two important agricultural products, namely, onion and potato. The irradiation process has the effect of reducing sprouting significantly. In Syria onions are usually stored in well ventilated warehouses at ambient temperature. Our investigation showed that sprouting started after 1.5 months of storage. It is estimated that the sprouting rate reaches 100% after five months of storage. This implies a waste factor of 50%. (author). 7 refs., 4 tabs

  3. Effect of Nano Iron and Solupotasse Fertilizers on Accumulation of Nutrient Elements and Quality of Two Onion (Allium cepa Cultivars

    Directory of Open Access Journals (Sweden)

    Ali Joghatay

    2015-11-01

    Full Text Available To study the effect of nano iron and solupotass on agronomic and physiological traits of two onion cultivars, a factorial experiment was conducted in complete randomized block design with 32 treatments and three replications in Joghatai of Khorasan-e- Razavi province, Iran. Treatments consisted of two onion cultivars (red, yellow and four levels (0, 1, 2, 3 kg per hectare of nano iron chelat and four levels of solupotass (0, 5, 10, 15 kg per hectare. Results showed that the effect of nano iron and solupotasse on fresh weight, dry weight, pyrovic acid and macro element (N, P, S contents were significant at %1 levels. Application nano iron, solupotasse to red onion cultivar increased dry weight significantly at the %5 level. Highest onion weight was obtained by using 2 kg nano iron and 15 kg solupotasse (17.3 g. Use of nano iron and solupotasse highly increased the pyruvic acid percentage (1.07 mM. Highest rate of pyruvic acid obtained by application of 3 and 15 kg nano iron and solupotasse respectively. Application of nano iron on the sulfur and nitrogen contents of onion were significant. Use of 2 kg/ha of nano iron exhibited highest increase in these elements. Thus, soil application of 10 kg/ha solupotasse, 3 kg/ha nano iron would highly increase red onion traits mentioned above.

  4. DEVELOPMENT AND COMPREHENSIVE ASSESSMENT OF BULBOUS FORMS OF INTERSPECIFIC HYBRIDS OF ONION ALLIUM CEPA x A. FISTULOSUM

    Directory of Open Access Journals (Sweden)

    V. S. Romanov

    2016-01-01

    Full Text Available One of the way of increasing of genetic variability of onion (Allium cepa L. is the interspecific hybridization. Development of onion interspecific hybrids consists of the study of initial breeding forms, its heterogeneity, ways of crossing and pollination, overcoming of outbreeding problem, sterility and weak fertility of the hybrids of first and next generations, specifics of hybrid’s seeds development, identification and selection of recombinant forms with breeding valuable traits. The stages of development of the bulbous forms of interspecific hybrids of onion are presented in the article. The study was conducted in the “All-Russian Scientific Research Institute of vegetable breeding and seed production” of the Moscow region. The plants of inbreed progenies I1-5 from BC1-2F5 of bulb forms of interspecific hybrids A. cepa х A. Fistulosum as well as the parental forms were analyzed. The breeding and phytopathological assessment of recombinant forms of onion interspecific hybrids was done for qualitative and quantitative traits and for resistance to downy mildew. Using the individual selection for quality and quantity traits, it was found that the forms, whose traits were not undergo the inbreeding depression because of the higher homozygosity can be used for development of linear initial material for breeding for heterosis. The forms with the inbreeding depression have to be used for crossbreeding. Along with increasing of homozygosity, the new modified genotypes appear because of potential variability and genes recombination. It allows to make the purposive selection of recombinant forms for valuable traits. The selected onion forms from inbreed progenies of I1-5 from BC1-2F5 which have bulbs of flat and well-rounded-flat shape are characterized by high resistance to downy mildew and bulbs with good storage ability. The selection process of the recombinant forms from progenies of onion interspecific hybrids obtained based on repeated

  5. Enhancement of phytochemical content and drying efficiency of onions (Allium cepa L.) through blanching.

    Science.gov (United States)

    Ren, Feiyue; Perussello, Camila A; Zhang, Zhihang; Gaffney, Michael T; Kerry, Joseph P; Tiwari, Brijesh K

    2018-03-01

    This study investigated the effect of blanching (60, 70 and 80 °C for 1, 3, 5 and 10 min) combined with oven drying at 60 °C on the phenolic compounds, antioxidant activity, colour and drying characteristics (drying time, drying rate constant, effective moisture diffusivity and activation energy) of onion slices. Blanching of onion slices at 60 °C for 3 min and at 70 °C for 1 min prior to drying increased their bioactive compounds and antioxidant activity compared to the control samples and other treatments. Eighteen drying models were evaluated. The Modified Page and two-term exponential models best represented the drying data. The effective diffusivity ranged from 3.32 × 10 -11 m 2 s -1 (control) to 5.27 × 10 -11 m 2 s -1 , 5.01 × 10 -11 m 2 s -1 , and 4.74 × 10 -11 m 2 s -1 for onions blanched at 60 °C, 70 °C and 80 °C, respectively. The higher activation energy was observed for the control (unblanched) sample and slightly lower values were found for 1 min- and 3 min-blanched samples, confirming the higher drying efficiency as a result of the blanching pre-treatment. The use of blanching as a pre-treatment before drying of onions resulted in enhanced phytochemical content and drying efficiency. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  6. Interactions between sanitizers and packaging gas compositions and their effects on the safety and quality of fresh-cut onions (Allium cepa L.).

    Science.gov (United States)

    Page, Natalie; González-Buesa, Jaime; Ryser, Elliot T; Harte, Janice; Almenar, Eva

    2016-02-02

    Onions are one of the most widely utilized vegetables worldwide, with demand for fresh-cut onions steadily increasing. Due to heightened safety concerns and consumer demand, the implications of sanitizing and packaging on fresh-cut onion safety and quality need to be better understood. The objective of this study was to investigate the effect of produce sanitizers, in-package atmospheres, and their interactions on the growth of Salmonella Typhimurium, mesophilic aerobic bacteria, yeast and mold, and the physico-chemical quality of diced onions to determine the best sanitizer and in-package atmosphere combination for both safety and quality. Diced onions were inoculated or not with S. Typhimurium, sanitized in sodium hypochlorite, peroxyacetic acid, or liquid chlorine dioxide, and then packaged in either polylactic acid bags containing superatmospheric O2, elevated CO2/reduced O2, or air, or in polyethylene terephthalate snap-fit containers. Throughout 14 days of storage at 7 °C, packaged diced onions were assessed for their safety (S. Typhimurium), and quality (mesophilic aerobic bacteria, yeasts and molds, physico-chemical analyses, and descriptive and consumer acceptance sensory panels). While sanitizer affected (Ppackage atmosphere had a significant (Pinteractions between sanitizer and atmosphere that affected S. Typhimurium and pH were identified whereas 3-way interactions (sanitizer, atmosphere and time) were only observed for headspace CO2. Sodium hypochlorite and elevated CO2/reduced O2 was the best sanitizer and in-package atmosphere combination for enhancing the safety and quality of packaged diced onions. In addition, this combination led to diced onions acceptable for purchase after 2 weeks of storage by trained and consumer panels. Copyright © 2015 Elsevier B.V. All rights reserved.

  7. Enzymatic production of pectic oligosaccharides from onion skins.

    Science.gov (United States)

    Babbar, Neha; Baldassarre, Stefania; Maesen, Miranda; Prandi, Barbara; Dejonghe, Winnie; Sforza, Stefano; Elst, Kathy

    2016-08-01

    Onion skins are evaluated as a new raw material for the enzymatic production of pectic oligosaccharides (POS) with a targeted degree of polymerization (DP). The process is based on a two-stage process consisting of a chelator-based crude pectin extraction followed by a controlled enzymatic hydrolysis. Treatment of the extracted crude onion skin's pectin with various enzymes (EPG-M2, Viscozyme and Pectinase) shows that EPG-M2 is the most appropriate enzyme for tailored POS production. The experiments reveal that the highest amount of DP2 and DP3 is obtained at a time scale of 75-90min with an EPG-M2 concentration of 26IU/mL. At these conditions the production amounts 2.5-3.0% (w/w) d.m for DP2 and 5.5-5.6% (w/w) d.m for DP3 respectively. In contrast, maximum DP4 production of 5.2-5.5% (w/w) d.m. is obtained with 5.2IU/mL at a time scale of 15-30min. Detailed LC-MS analysis reveals the presence of more methylated oligomers compared to acetylated forms in the digests. Copyright © 2016 Elsevier Ltd. All rights reserved.

  8. A Customized Metal Oxide Semiconductor-Based Gas Sensor Array for Onion Quality Evaluation: System Development and Characterization

    Directory of Open Access Journals (Sweden)

    Tharun Konduru

    2015-01-01

    Full Text Available A gas sensor array, consisting of seven Metal Oxide Semiconductor (MOS sensors that are sensitive to a wide range of organic volatile compounds was developed to detect rotten onions during storage. These MOS sensors were enclosed in a specially designed Teflon chamber equipped with a gas delivery system to pump volatiles from the onion samples into the chamber. The electronic circuit mainly comprised a microcontroller, non-volatile memory chip, and trickle-charge real time clock chip, serial communication chip, and parallel LCD panel. User preferences are communicated with the on-board microcontroller through a graphical user interface developed using LabVIEW. The developed gas sensor array was characterized and the discrimination potential was tested by exposing it to three different concentrations of acetone (ketone, acetonitrile (nitrile, ethyl acetate (ester, and ethanol (alcohol. The gas sensor array could differentiate the four chemicals of same concentrations and different concentrations within the chemical with significant difference. Experiment results also showed that the system was able to discriminate two concentrations (196 and 1964 ppm of methlypropyl sulfide and two concentrations (145 and 1452 ppm of 2-nonanone, two key volatile compounds emitted by rotten onions. As a proof of concept, the gas sensor array was able to achieve 89% correct classification of sour skin infected onions. The customized low-cost gas sensor array could be a useful tool to detect onion postharvest diseases in storage.

  9. Garlic and onion sensitization among Saudi patients screened for ...

    African Journals Online (AJOL)

    EB

    2013-09-03

    Sep 3, 2013 ... screened for garlic and onion specific IgE antibodies along with other food allergens were analyzed retrospectively at King. Khalid University Hosptial between January 2008 and April 2009. This group of patients included 73 males and 35 females with mean age 27+13.2 years. Estimation of garlic and ...

  10. Pressurised liquid extraction of flavonoids in onions. Method development and validation

    DEFF Research Database (Denmark)

    Søltoft, Malene; Christensen, J.H.; Nielsen, J.

    2009-01-01

    A rapid and reliable analytical method for quantification of flavonoids in onions was developed and validated. Five extraction methods were tested on freeze-dried onions and subsequently high performance liquid chromatography (HPLC) with UV detection was used for quantification of seven flavonoids...... extraction methods. However. PLE was the preferred extraction method because the method can be highly automated, use only small amounts of solvents, provide the cleanest extracts, and allow the extraction of light and oxygen-sensitive flavonoids to be carried out in an inert atmosphere protected from light......-step PLE method showed good selectivity, precision (RSDs = 3.1-11%) and recovery of the extractable flavonoids (98-99%). The method also appeared to be a multi-method, i.e. generally applicable to, e.g. phenolic acids in potatoes and carrots....

  11. Fertilizer nitrogen recovery by onion (Allium cepa) as influenced by N and S application in a sulphur deficient soil

    International Nuclear Information System (INIS)

    Sachdev, M.S.; Sachdev, P.; Mittal, R.B.; Luthra, V.K.

    1991-01-01

    A field experiment was conducted in a sulphur deficient sandy loam soil to evaluate the fertilizer N-utilization by onion (Allium cepa) using 15 N-labelled urea. The fresh bulb yield of onion significantly increased with the application of both nitrogen and sulphur. The recovery of fertilizer N by onion was 23.4 to 34.4 per cent at 60 kg N/ha and between 17.5 and 19.3 per cent at 120 kg/ha level. Application of nitrogen with sulphur at 20 kg S/ha through gypsum resulted in significant increase in per cent utilization of urea nitrogen. Nearly 57 to 68 per cent of the residual fertilizer N could be traced in soil after the harvest of onion crop, whereas, 9 to 14 per cent of the applied fertilizer N could not be accounted for. (author). 8 refs., 3 tabs., 2 figs

  12. Morphological Performance of Onion under Exogenous Treatments of GA3

    Directory of Open Access Journals (Sweden)

    Md.Dulal SARKAR

    2018-03-01

    Full Text Available The present study was conducted to assess the morphological response of onion plants to different GA3 levels (0, 20, 40 and 60 ppm. The factor levels of GA3 were applied during transplanting by root soaking and foliar spray at 30 and 60 days after transplanting. The gibberellic acid had a great effect on increasing plant height (46.50 cm, shoot biomass (641.67 g m-2, bulb biomass (1125.00 g m-2 and also dry matter accumulation in onion plants under the effect of 60 ppm compared to control. Plants grown up without GA3 application were shorter than those grown with GA3 spray where the lowest plant height (34.67 cm was remarked. The leaf number (11.43 was considerably increased when 60 ppm GA3 was used as the growth promoter factor in comparison to control. The plants attain minimum fresh biomass at harvesting time in the shoot (441.67 g m-2 and bulb (641.67 g m-2 grown in control plot. Considerably (41.63% more dry shoot biomass accumulation was recorded in 60 ppm GA3 treated plants in comparison with the control at harvesting stages. Insignificant effect by all concentration of GA3 was found in bulb length, fresh root biomass and dry root biomass. Thus, the use of 60 ppm GA3 can be recommend for onion production due to the significantly increased of the fresh bulb biomass with about 42.96% over control.

  13. Young, intact and nested retrotransposons are abundant in the onion and asparagus genomes.

    Science.gov (United States)

    Vitte, C; Estep, M C; Leebens-Mack, J; Bennetzen, J L

    2013-09-01

    Although monocotyledonous plants comprise one of the two major groups of angiosperms and include >65 000 species, comprehensive genome analysis has been focused mainly on the Poaceae (grass) family. Due to this bias, most of the conclusions that have been drawn for monocot genome evolution are based on grasses. It is not known whether these conclusions apply to many other monocots. To extend our understanding of genome evolution in the monocots, Asparagales genomic sequence data were acquired and the structural properties of asparagus and onion genomes were analysed. Specifically, several available onion and asparagus bacterial artificial chromosomes (BACs) with contig sizes >35 kb were annotated and analysed, with a particular focus on the characterization of long terminal repeat (LTR) retrotransposons. The results reveal that LTR retrotransposons are the major components of the onion and garden asparagus genomes. These elements are mostly intact (i.e. with two LTRs), have mainly inserted within the past 6 million years and are piled up into nested structures. Analysis of shotgun genomic sequence data and the observation of two copies for some transposable elements (TEs) in annotated BACs indicates that some families have become particularly abundant, as high as 4-5 % (asparagus) or 3-4 % (onion) of the genome for the most abundant families, as also seen in large grass genomes such as wheat and maize. Although previous annotations of contiguous genomic sequences have suggested that LTR retrotransposons were highly fragmented in these two Asparagales genomes, the results presented here show that this was largely due to the methodology used. In contrast, this current work indicates an ensemble of genomic features similar to those observed in the Poaceae.

  14. Study of Nutrient Content Variation in Bulb And Stalk of Onions ...

    African Journals Online (AJOL)

    acer

    Keywords: Onion bulbs and stalks, proximate, nutrient elements, vitamins A and C; oxalate. ... application of fertilizers, manure, compost and ..... iron products, the use of natural source of Fe such .... in Fish and Sediment from Kubanni River,.

  15. A Case of Profound Soft Tissue Infection of Lower Limb Contained Maggots after IV Abuse of Iranian

    Directory of Open Access Journals (Sweden)

    Majid Heidary

    2016-07-01

    Full Text Available Background: Infections include soft tissue infections are notable reason for hospital admission among IDUs, owing to unsterile injection techniques and equipment, contamination of drugs with organisms, and poor hygiene. In present case report a patient with profound limb infection is introduced. Case: A 32 years old man was transferred to the emergency department. He was IDU with Iranian for 3 years. Gangrenous deformity of left lower extremity below the knee was seen. Live maggots were moving around the limb freely. The patient underwent before knee amputation (BKA to remove the infected tissues of the limb. Conclusion: In order to evaluate and treat the serious infectious disease problems, drug abuse treatment programs will need to develop appropriate procedures. It is important that physicians, nurses, and other health care providers become better educated about drug abuse. Workers in drug abuse treatment should be well informed about infectious diseases and other complications of drug abuse.

  16. Investigation of Volatiles Emitted from Freshly Cut Onions (Allium cepa L. by Real Time Proton-Transfer Reaction-Mass Spectrometry (PTR-MS

    Directory of Open Access Journals (Sweden)

    Mette Marie Løkke

    2012-11-01

    Full Text Available Volatile organic compounds (VOCs in cut onions (Allium cepa L. were continuously measured by PTR-MS during the first 120 min after cutting. The headspace composition changed rapidly due to the very reactive volatile sulfurous compounds emitted from onion tissue after cell disruption. Mass spectral signals corresponding to propanethial S-oxide (the lachrymatory factor and breakdown products of this compound dominated 0–10 min after cutting. Subsequently, propanethiol and dipropyl disulfide predominantly appeared, together with traces of thiosulfinates. The concentrations of these compounds reached a maximum at 60 min after cutting. Propanethiol was present in highest concentrations and had an odor activity value 20 times higher than dipropyl disulfide. Thus, propanethiol is suggested to be the main source of the characteristic onion odor. Monitoring the rapid changes of VOCs in the headspace of cut onion necessitates a high time resolution, and PTR-MS is demonstrated to be a very suitable method for monitoring the headspace of freshly cut onions directly after cutting without extraction or pre-concentration.

  17. Pathogenicity for onion and genetic diversity of isolates of the pathogenic fungus Colletotrichum gloeosporioides (Phyllachoraceae) from the State of Pernambuco, Brazil.

    Science.gov (United States)

    Nova, M X Vila; Borges, L R; de Sousa, A C B; Brasileiro, B T R V; Lima, E A L A; da Costa, A F; de Oliveira, N T

    2011-02-22

    Onion anthracnose, caused by Colletotrichum gloeosporioides, is one of the main diseases of onions in the State of Pernambuco. We examined the pathogenicity of 15 C. gloeosporioides strains and analyzed their genetic variability using RAPDs and internal transcribed spacers (ITS) of the rDNA region. Ten of the strains were obtained from substrates and hosts other than onion, including chayote (Sechium edule), guava (Psidium guajava), pomegranate (Punica granatum), water from the Capibaribe River, maracock (Passiflora sp), coconut (Cocus nucifera), surinam cherry (Eugenia uniflora), and marine soil; five isolates came from onions collected from four different regions of the State of Pernambuco and one region of the State of Amazonas. Pathogenicity tests were carried out using onion leaves and bulbs. All strains were capable of causing disease in leaves, causing a variable degree of lesions on the leaves; four strains caused the most severe damage. In the onion bulb tests, only three of the above strains caused lesions. Seven primers of arbitrary sequences were used in the RAPD analysis, generating polymorphic bands that allowed the separation of the strains into three distinct groups. The amplification products generated with the primers ITS1 and ITS4 also showed polymorphism when digested with three restriction enzymes, DraI, HaeIII and MspI. Only the latter two demonstrated genetic variations among the strains. These two types of molecular markers were able to differentiate the strain from the State of Amazonas from those of the State of Pernambuco. However, there was no relationship between groups of strains, based on molecular markers, and degree of pathogenicity for onion leaves and bulbs.

  18. Raman spectroscopy study of the nanodiamond-to-carbon onion transformation

    International Nuclear Information System (INIS)

    Cebik, Jonathan; Peerally, Filipe; Medrano, Rene; Osswald, Sebastian; McDonough, John K; Neitzel, Ioannis; Gogotsi, Yury

    2013-01-01

    Here, we present a comprehensive study analyzing early stages of the transformation of detonation nanodiamond (ND) powder to graphitic carbon onions via thermal annealing in argon atmosphere. Raman spectroscopy was employed to monitor this transformation, starting with the sp 3 -to-sp 2 conversion of the ND surface at the onset of the graphitization process. Additionally, transmission electron microscopy, x-ray diffraction, and thermogravimetric analysis were used to supplement the structural information obtained from Raman spectroscopy and allow for an accurate interpretation of the obtained Raman data. The effect of the annealing time on the transformation process was also studied to determine the kinetics of the conversion at low temperatures. The results presented in this study complement previous work on ND annealing and provide deeper insight into the nanodiamond-to-carbon onion conversion mechanism, in particular the time and size dependence. We present further evidence for the existence of a disordered sp 2 phase as an intermediate step in the transformation process. (paper)

  19. Onion Hybrid Seed Production: Relation with Nectar Composition and Flower Traits.

    Science.gov (United States)

    Soto, Veronica C; Caselles, Cristian A; Silva, Maria F; Galmarini, Claudio R

    2018-05-28

    Onion (Allium cepa L.) is one of the main vegetable crops. Pollinators are required for onion seed production, being honeybees the most used. Around the world, two types of onion varieties are grown: open pollinated (OP) and hybrids. Hybrids offer numerous advantages to growers, but usually have lower seed yields than OP cultivars, which in many cases compromise the success of new hybrids. As pollination is critical for seed set, understanding the role of floral rewards and attractants to pollinator species is the key to improve crop seed yield. In this study, the correlation of nectar-analyzed compounds, floral traits, and seed yield under open field conditions in two experimental sites was determined. Nectar composition was described through the analysis of sugars, phenol, and alkaloid compounds. Length and width of the style and tepals of the flowers were measured to describe floral traits. Floral and nectar traits showed differences among the studied lines. For nectar traits, we found a significant influence of the environment where plants were cultivated. Nonetheless, flower traits were not influenced by the experimental sites. The OP and the male-sterile lines (MSLs) showed differences in nectar chemical composition and floral traits. In addition, there were differences between and within MSLs, some of which were correlated with seed yield, bringing the opportunity to select the most productive MSL, using simple determinations of morphological characters like the length of the style or tepals size.

  20. Feasibility of radiation preservation of potatoes, onions and garlic in Pakistan

    International Nuclear Information System (INIS)

    Khan, I.; Wahid, M.

    1978-01-01

    The paper describes a study conducted to explore the possibility of extending the storage life of potatoes, onions and garlic, which pose a serious storage problem in the hot summer months. Potatoes of the Ultimus and Desiree varieties, stored at ambient room temperature (25-40 0 C), R.H. 40-50% and irrespective of the treatment, started rotting only two weeks after storage began and all the lots were completely destroyed by rot attack after a storage period of 12 weeks. The irradiated tubers (10krad) of both Ultimus and Desiree, stored at low temperature (14-16 0 C) and R.H. 60-70%, showed complete inhibition of sprouting. Sprouting was completely inhibited in irradiated onions (10krad) stored at ambient and low temperature during the whole storage period. The extent of loss due to rot attack after 150 days of storage at room temperature was 35 and 36% in control and irradiated bulbs, whereas at low temperature this loss was 22 and 15% respectively. Irradiated garlic (10krad), stored at room and low temperatures, showed complete inhibition of sprouting up to 6 months. However, the rot attack in control and irradiated bulbs stored at ambient room temperature was 46 and 54% respectively. It is concluded that radiation followed by storage at 14-16 0 C is the optimum condition for storing potatoes, onions and garlic in good condition for more than 5 months in tropical countries like Pakistan. (author)

  1. Identification of candidate amino acids involved in the formation of pink-red pigments in onion (Allium cepa L.) juice and separation by HPLC.

    Science.gov (United States)

    Lee, Eun Jin; Yoo, Kil Sun; Patil, Bhimanagouda S

    2010-10-01

    The formation of pink-red pigments ("pinking") by various amino acids was investigated by reacting amino acids with compounds present in onion juice. The unknown pink-red pigments were generated and separated using high-performance liquid chromatography (HPLC) and a diode array detector (DAD) in the range of 200 to 700 nm. To generate pink-red pigments, we developed several reaction systems using garlic alliinase, purified 1-propenyl-L-cysteine sulfoxide (1-PeCSO), onion thiosulfinate, natural onion juice, and 21 free amino acids. The compound 1-PeCSO was a key compound associated with pinking in the presence of both the alliinase and amino acids. Numerous naturally occurring pink-red pigments were detected and separated from pink onion juice using the HPLC-DAD system at 515 nm. Most free amino acids, with the exceptions of histidine, serine, and cysteine, formed various pink-red pigments when reacted with onion thiosulfinate. This observation indicated that onion pinking is caused not by a single pigment, but by many. Furthermore, more than one color compound could be produced from a single amino acid; this explains, in part, why there were many pink-red compound peaks in the chromatogram of discolored natural onion juice. We presumed that the complexity of the pink-red pigments was due to the involvement of more than 21 natural amino acids as well as several derivatives of the color products produced from each amino acid. We observed that the pinking process in onion juice is very similar to that of the greening process in crushed garlic, emphasizing that both thiosulfinate from flavor precursors and free amino acids are absolutely required for the discoloration.

  2. Response of Onion ( Allium cepa L.) to Irrigation Intervals and Plant ...

    African Journals Online (AJOL)

    08 at the Teaching and Research Farm of the College of Agriculture, Zuru, Kebbi State, Nigeria. The objective was to investigate the response of onion to irrigation interval and plant population density. The treatments consisted of factorial ...

  3. Effect of Aqueous Allium Cepa Extract from Red Onion on Aluminum ...

    African Journals Online (AJOL)

    ADOWIE PERE

    2017-12-14

    Dec 14, 2017 ... ABSTRACT: This study examined the protective effect of Allium cepa extract from red onion on aluminum – ... permits unrestricted use, distribution, and reproduction in any .... water, air dried, and 200 g was then blended. The.

  4. Identification of cadmium-excluding Welsh onion (Allium fistulosum L.) cultivars and their mechanisms of low cadmium accumulation.

    Science.gov (United States)

    Li, Xuhui; Zhou, Qixing; Wei, Shuhe; Ren, Wenjie

    2012-06-01

    Screening out cadmium (Cd) excluding cultivars of a crop in agricultural production is an effective way to prohibit Cd entering into food chain. A judging criterion for Cd-excluding cultivars based on food safety was suggested and used in the identification of Cd-excluding welsh onion (Allium fistulosum L.) cultivars. A pot culture experiment was carried out to screen out Cd-excluding cultivars, of which the results were confirmed by plot experiments. The relevant factors of Cd accumulation in the pseudostem were analyzed and used in the correlation analysis aiming to study the low Cd accumulation mechanisms. The concentration of Cd in the pseudostem of welsh onions was 0.08-0.20, 0.18-0.41, and 0.26-0.61 mg/kg fresh weight (FW) under three treatments (1.0, 2.5, and 5.0 mg/kg), respectively. The significant (p onion cultivars, but Cd contamination in soil had little influence on biomass and the contents of soluble sugar, NO(3)(-)-N, and eight other elements in the tested welsh onion cultivars. Two cultivars were identified as Cd-excluding cultivars, mainly because the accumulation of Cd in their pseudostem was only 0.041 ± 0.003 and 0.046 ± 0.002 mg/kg FW, and 0.054 ± 0.001 and 0.066 ± 0.011 mg/kg FW, when growing in plots with Cd concentration of 0.49 and 0.99 mg/kg, respectively. Ribentiegancongwang and Wuyeqi could be identified as Cd-excluding cultivars. Low bioaccumulation factor of the roots was the main mechanism of Cd-excluding welsh onion cultivars.

  5. Survival or growth of inoculated Escherichia coli O157:H7 and Salmonella on yellow onions (Allium cepa) under conditions simulating food service and consumer handling and storage.

    Science.gov (United States)

    Lieberman, Vanessa M; Zhao, Irene Y; Schaffner, Donald W; Danyluk, Michelle D; Harris, Linda J

    2015-01-01

    Whole and diced yellow onions (Allium cepa) were inoculated with five-strain cocktails of rifampin-resistant Escherichia coli O157:H7 or Salmonella and stored under conditions to simulate food service or consumer handling. The inoculum was grown in broth (for both whole and diced onion experiments) or on agar plates (for whole onion experiments). Marked circles (3.3 cm in diameter) on the outer papery skin of whole onions were spot inoculated (10 μl in 10 drops) at 7 log CFU per circle, and onions were stored at 4°C, 30 to 50 % relative humidity, or at ambient conditions (23°C, 30 to 50 % relative humidity). Diced onions were inoculated at 3 log CFU/g and then stored in open or closed containers at 4°C or ambient conditions. Previously inoculated and ambient-stored diced onions were also mixed 1:9 (wt/wt) with refrigerated uninoculated freshly diced onions and stored in closed containers at ambient conditions. Inoculated pathogens were recovered in 0.1 % peptone and plated onto selective and nonselective media supplemented with 50 μg/ml rifampin. Both E. coli O157:H7 and Salmonella populations declined more rapidly on onion skins when the inoculum was prepared in broth rather than on agar. Agar-prepared E. coli O157:H7 and Salmonella declined by 0.4 and 0.3 log CFU per sample per day, respectively, at ambient conditions; at 4°C the rates of reduction were 0.08 and 0.06 log CFU per sample per day for E. coli O157:H7 and Salmonella, respectively. Populations of E. coli O157:H7 and Salmonella did not change over 6 days of storage at 4°C in diced onions. Lag times of 6 to 9 h were observed with freshly inoculated onion at ambient conditions; no lag was observed when previously inoculated and uninoculated onions were mixed. Growth rates at ambient conditions were 0.2 to 0.3 log CFU/g/h for E. coli O157:H7 and Salmonella in freshly inoculated onion and 0.2 log CFU/g/h in mixed product. Diced onions support pathogen growth and should be kept refrigerated.

  6. A Model of Onion Routing With Provable Anonymity

    Science.gov (United States)

    2006-08-30

    Lysyanskaya. “A Formal Treatment of Onion Routing.” CRYPTO 2005, pp. 169.187, 2005. [4] David Chaum . “The dining cryptographers problem...1988. [5] David Chaum . “Untraceable Electronic Mail, Return Addresses, and Digital Pseudonyms.” Communi- cations of the ACM, 24(2), pp. 84-88, 1981...network layer.” ACM Conference on Computer and Communications Security, pp. 193-206, 2002. [11] David Goldschlag, Michael Reed, and Paul Syverson

  7. Investigations of radiation stimulation of tomatoes, onions and radishes in climatic chamber and field conditions

    International Nuclear Information System (INIS)

    Shimon, J.; Pal, I.

    1974-01-01

    The results of three years' experiments with presowing irradiation of tomato, radish, and onion seeds are reported. The main criteria used, were the following: plant height, change in flowering time, number of natured fruits per plant, total carbohydrate content in ripe fruits and of vitamin C level in natured fruits for tomatoes; tuber weight, weight of leaves, total weight, dry matter formation, and contents of carbohydrates and vitamin C for radish; and appearance of flower ''arrows'', yield, and dry matter content for onions. (E.T.)

  8. Radiation preservation of foods of plant origin. Part 2. Onions and other bulb crops

    International Nuclear Information System (INIS)

    Thomas, P.

    1984-01-01

    The various factors contributing to post harvest losses in onions and other bulb crops are briefly outlined in terms of the current storage methods. The present status of research on sprout inhibition by irradiation is reviewed in detail with respect to dose requirements, effect of time interval between harvest and irradiation, and the influence of environment on sprouting during storage. Biochemical mechanisms of sprout inhibition, metabolic and compositional changes (particularly sugars, anthocyanins, flavor and lachrymatory principles), and the culinary and processing qualities of irradiated onions are discussed. The future prospects for the commercial irradiation for sprout inhibition of bulb crops are considered

  9. Storage rot fungi and seed-borne pathogens of onion | Adongo ...

    African Journals Online (AJOL)

    Rotten onion bulbs obtained from the four markets (Abinchi, Anloga, Kwadaso and Central markets) were infected by five fungal species: Aspergillus niger, A. flavus, Penicillium sp., Rhizopus stolonifer and Fusarium oxysporum. Of these, A. niger and Penicillium sp. were the most frequently isolated fungi. A. flavus was the ...

  10. THE PROTECTIVE ROLE OF ONION OIL (ALLIUM CEPA LINN) AGAINST RADIATION-INDUCED HAZARDS IN MALE ALBINO RATS

    International Nuclear Information System (INIS)

    HAMZA, R.G.

    2008-01-01

    Radiation poses a major currently irresolvable risk for human. Onion is a major source of dietary flavonoids. The present investigation was carried out to study the protective effects of treating rats with onion oil (150 mg/kg body weight) for consecutive 3 weeks against damages induced by whole body gamma irradiation (7 Gy). Exposure of rats to gamma irradiation caused a significant increase in levels of serum glucose, cholesterol, triglycerides as well as activities of AST, ALT, alkaline phosphatase, creatinine, uric acid and lipid peroxides. Exposure to gamma rays resulted in an increase in the mentioned parameters accompanied by a decrease in urea, total protein, albumin, glutathione content, superoxide dismutase and catalase activities. It could be concluded that onion oil capable of reducing the biological hazards induced by gamma irradiation

  11. Consumption of Fresh Yellow Onion Ameliorates Hyperglycemia and Insulin Resistance in Breast Cancer Patients During Doxorubicin-Based Chemotherapy: A Randomized Controlled Clinical Trial.

    Science.gov (United States)

    Jafarpour-Sadegh, Farnaz; Montazeri, Vahid; Adili, Ali; Esfehani, Ali; Rashidi, Mohammad-Reza; Pirouzpanah, Saeed

    2017-09-01

    Doxorubicin has been found to be associated with insulin resistance in animal models. Onion, a so-called functional food, is noted to affect the insulin signaling pathway of diabetes in vitro. To our knowledge, this is the first study to investigate the effects of consuming fresh yellow onions on insulin-related indices compared with a low-onion-containing diet among breast cancer (BC) patients treated with doxorubicin. This parallel-design, randomized, triple-blind, controlled clinical trial was conducted on 56 eligible BC patients (aged 30-63 years), diagnosed with invasive ductal carcinoma. Following their second cycle of chemotherapy, subjects were assigned in a stratified-random allocation to receive body mass index-dependent 100 to 160 g/d of onion as high onion group (HO; n = 28) or 30 to 40 g/d small onions in low onion group (LO; n = 28) for 8 weeks intervention. Participants, care givers, and those who assessed laboratory analyses were blinded to the assignments (IRCT Registry No.: IRCT2012103111335N1). The compliance level of participants in the analysis was as high as 87.85%. A total of 23 available cases was analyzed in each group. The daily use of HO resulted in a significant decrease in serum fasting blood glucose and insulin levels in comparison with LO, over the period of study ( P insulin resistance relative to changes in the LO group ( P insulin sensitivity check index ( P insulin resistance in BC during doxorubicin-based chemotherapy.

  12. Detection of irradiated onion by means of the comet assay

    International Nuclear Information System (INIS)

    Moreno Alvarez, Damaris L.; Prieto Miranda, Enrique Fco.; Carro Palacio, Sandra; Iglesia Enriquez, Isora

    2007-01-01

    The ionizing radiations are used as a harmless alternative treatment that it substitutes the employment of chemical treatments, which after their application in the food products can remain residuals not desired that they come to be carcinogenic. With the food irradiation is eliminated microorganisms and the storage time is prolonged, which produces benefits for the Food Industry and the consumers. In many countries the search of sensitive detecting methods of irradiated foods is promoted by the necessity of the assurance of the consumption of foods with nutritional quality and to test directly the radiation processing, for which several techniques have been developed, these are based on the changes that induce the ionizing radiations in the food products. A recommended method is the Comet Assay of DNA, it is approved by the European Committee of Standardization (EN 13784). The DNA molecule is very sensitive to gamma radiations even at low radiation dose, where the modifications produced in the molecule can be monitored for this analytical technique well-known as Comet Assay of DNA or Single Cell Gel Electrophoresis. The objective of the present paper was to evaluate the modifications of the DNA molecule of irradiated onions with the Comet Assay for several dose values, the onions were conserved at environment and refrigeration temperatures. The samples were irradiated in a self-shielding irradiator with 60 Co source, dose rate of 20.45 Gy/min and absorbed dose values of 0.5; 0.6; 0.8 and 1.0 kGy. This detection method demonstrates to be one sensitive and quick technique for the qualitative detection of irradiated onions. (author)

  13. Light Intensity and Carbon Dioxide Availability Impact Antioxidant Activity in Green Onions (Allium fistulosumm L)

    Science.gov (United States)

    Levine, Lanfang; Bisbee, Patricia; Pare, Paul

    The prospect of long-duration manned space missions poses many challenges, including the development of a sustainable life support system and effective methods of space-radiation protection. To mitigate the risk of increased space-radiation, functional foods rich in antioxidant properties such as green onions are of particular interest. However it has yet to be established whether antioxidant properties can be preserved or enhanced in space environment where carbon dioxide, lighting intensity, gravity and pressure differ from which plants have acclimated to on earth. In this study, green onions (Allium fistulosumm L. cultivar Kinka) rich in antioxidant flavonoids are used as a model system to investigate variations in antioxidant capacity with plants grown under varying light intensities and CO2 concentrations. The antioxidant potential is determined using both radical cation scavenging and oxygen radical absorbance assays. For all light intensities assayed, antioxidant potential in water extract of green onions per gram biomass declined with CO2 increases up to 1200 ppm, and then leveled off with further CO2 increase to 4000 ppm. This inverse carbon dioxide versus antioxidant activity correlation suggests lower accumulation rates for water soluble antioxidant compounds compared to total biomass under increasing CO2 concentrations. The effect of increasing atmospheric CO2 concentration on antioxidant activity of ethanol extracts were light intensity dependent. The implications of these findings are discussed in the context of traditional plant antioxidants including vitamin C and the major onion flavonoid quercetin.

  14. Effect of gamma irradiation on the storage and vitamin C concentration of allium cepa onion samples

    International Nuclear Information System (INIS)

    Saleh, M. A. M.

    2013-07-01

    This study was done to investigate the effect of gamma irradiation on storage, germination and vitamin C concentration of local onion (Allium cepa). 5 onion samples were irradiated with 5 different radiation doses (0.1, 0.2, 0.3, 0.4, and 0.5 k Gray) using cobalt-60 irradiator (Nor din) compared to non irradiated samples as controls. The irradiated and control onions were stored at room temperature for three months. The results of this study showed that the non irradiated samples were either deteriorated or grown while all the irradiated samples were not. Regarding the concentration of vitamin C it was clear that it decreased with the dose increase from 30.53 to 14.44 mg/100 g. This study concluded that the irradiation is very effective in prevention of spoilage, elongation of germination period and decrease of vitamin C concentration.(Author)

  15. Insecticide use in hybrid onion seed production affects pre- and postpollination processes.

    Science.gov (United States)

    Gillespie, Sandra; Long, Rachael; Seitz, Nicola; Williams, Neal

    2014-02-01

    Research on threats to pollination service in agro-ecosystems has focused primarily on the negative impacts of land use change and agricultural practices such as insecticide use on pollinator populations. Insecticide use could also affect the pollination process, through nonlethal impacts on pollinator attraction and postpollination processes such as pollen viability or pollen tube growth. Hybrid onion seed (Allium cepa L., Alliaceae) is an important pollinator-dependent crop that has suffered yield declines in California, concurrent with increased insecticide use. Field studies suggest that insecticide use reduces pollination service in this system. We conducted a field experiment manipulating insecticide use to examine the impacts of insecticides on 1) pollinator attraction, 2) pollen/stigma interactions, and 3) seed set and seed quality. Select insecticides had negative impacts on pollinator attraction and pollen/stigma interactions, with certain products dramatically reducing pollen germination and pollen tube growth. Decreased pollen germination was not associated with reduced seed set; however, reduced pollinator attraction was associated with lower seed set and seed quality, for one of the two female lines examined. Our results highlight the importance of pesticide effects on the pollination process. Overuse may lead to yield reductions through impacts on pollinator behavior and postpollination processes. Overall, in hybrid onion seed production, moderation in insecticide use is advised when controlling onion thrips, Thrips tabaci, on commercial fields.

  16. The Onion (Allium cepa L.) R2R3-MYB Gene MYB1 Regulates Anthocyanin Biosynthesis

    Science.gov (United States)

    Schwinn, Kathy E.; Ngo, Hanh; Kenel, Fernand; Brummell, David A.; Albert, Nick W.; McCallum, John A.; Pither-Joyce, Meeghan; Crowhurst, Ross N.; Eady, Colin; Davies, Kevin M.

    2016-01-01

    Bulb color is an important consumer trait for onion (Allium cepa L., Allioideae, Asparagales). The bulbs accumulate a range of flavonoid compounds, including anthocyanins (red), flavonols (pale yellow), and chalcones (bright yellow). Flavonoid regulation is poorly characterized in onion and in other plants belonging to the Asparagales, despite being a major plant order containing many important crop and ornamental species. R2R3-MYB transcription factors associated with the regulation of distinct branches of the flavonoid pathway were isolated from onion. These belonged to sub-groups (SGs) that commonly activate anthocyanin (SG6, MYB1) or flavonol (SG7, MYB29) production, or repress phenylpropanoid/flavonoid synthesis (SG4, MYB4, MYB5). MYB1 was demonstrated to be a positive regulator of anthocyanin biosynthesis by the induction of anthocyanin production in onion tissue when transiently overexpressed and by reduction of pigmentation when transiently repressed via RNAi. Furthermore, ectopic red pigmentation was observed in garlic (Allium sativum L.) plants stably transformed with a construct for co-overexpression of MYB1 and a bHLH partner. MYB1 also was able to complement the acyanic petal phenotype of a defined R2R3-MYB anthocyanin mutant in Antirrhinum majus of the asterid clade of eudicots. The availability of sequence information for flavonoid-related MYBs from onion enabled phylogenetic groupings to be determined across monocotyledonous and dicotyledonous species, including the identification of characteristic amino acid motifs. This analysis suggests that divergent evolution of the R2R3-MYB family has occurred between Poaceae/Orchidaceae and Allioideae species. The DNA sequences identified will be valuable for future analysis of classical flavonoid genetic loci in Allium crops and will assist the breeding of these important crop species. PMID:28018399

  17. The onion (Allium cepa L. R2R3-MYB gene MYB1 regulates anthocyanin biosynthesis

    Directory of Open Access Journals (Sweden)

    Kathy Schwinn

    2016-12-01

    Full Text Available Bulb colour is an important consumer trait for onion (Allium cepa L., Allioideae, Asparagales. The bulbs accumulate a range of flavonoid compounds, including anthocyanins (red, flavonols (pale yellow and chalcones (bright yellow. Flavonoid regulation is poorly characterised in onion and in other plants belonging to the Asparagales, despite being a major plant order containing many important crop and ornamental species. R2R3-MYB transcription factors associated with the regulation of distinct branches of the flavonoid pathway were isolated from onion. These belonged to sub-groups (SGs that commonly activate anthocyanin (SG6, MYB1 or flavonol (SG7, MYB29 production, or repress phenylpropanoid/flavonoid synthesis (SG4, MYB4, MYB5. MYB1 was demonstrated to be a positive regulator of anthocyanin biosynthesis by the induction of anthocyanin production in onion tissue when transiently overexpressd and by reduction of pigmentation when transiently repressed via RNAi. Furthermore, ectopic red pigmentation was observed in garlic (A. sativum L. plants stably transformed with a construct for co-overexpression of MYB1 and a bHLH partner. MYB1 also was able to complement the acyanic petal phenotype of a defined R2R3-MYB anthocyanin mutant in Antirrhinum majus of the asterid clade of eudicots. The availability of sequence information for flavonoid-related MYBs from onion enabled phylogenetic groupings to be determined across monocotyledonous and dicotyledonous species, including the identification of characteristic amino acid motifs. This analysis suggests that divergent evolution of the R2R3-MYB family has occurred between Poaceae/Orchidaceae and Allioideae species. The DNA sequences identified will be valuable for future analysis of classical flavonoid genetic loci in Allium crops and will assist the breeding of these important crop species.

  18. The Onion (Allium cepa L.) R2R3-MYB Gene MYB1 Regulates Anthocyanin Biosynthesis.

    Science.gov (United States)

    Schwinn, Kathy E; Ngo, Hanh; Kenel, Fernand; Brummell, David A; Albert, Nick W; McCallum, John A; Pither-Joyce, Meeghan; Crowhurst, Ross N; Eady, Colin; Davies, Kevin M

    2016-01-01

    Bulb color is an important consumer trait for onion ( Allium cepa L., Allioideae, Asparagales). The bulbs accumulate a range of flavonoid compounds, including anthocyanins (red), flavonols (pale yellow), and chalcones (bright yellow). Flavonoid regulation is poorly characterized in onion and in other plants belonging to the Asparagales, despite being a major plant order containing many important crop and ornamental species. R2R3-MYB transcription factors associated with the regulation of distinct branches of the flavonoid pathway were isolated from onion. These belonged to sub-groups (SGs) that commonly activate anthocyanin (SG6, MYB1) or flavonol (SG7, MYB29) production, or repress phenylpropanoid/flavonoid synthesis (SG4, MYB4, MYB5). MYB1 was demonstrated to be a positive regulator of anthocyanin biosynthesis by the induction of anthocyanin production in onion tissue when transiently overexpressed and by reduction of pigmentation when transiently repressed via RNAi. Furthermore, ectopic red pigmentation was observed in garlic ( Allium sativum L.) plants stably transformed with a construct for co-overexpression of MYB1 and a bHLH partner. MYB1 also was able to complement the acyanic petal phenotype of a defined R2R3-MYB anthocyanin mutant in Antirrhinum maju s of the asterid clade of eudicots. The availability of sequence information for flavonoid-related MYBs from onion enabled phylogenetic groupings to be determined across monocotyledonous and dicotyledonous species, including the identification of characteristic amino acid motifs. This analysis suggests that divergent evolution of the R2R3-MYB family has occurred between Poaceae/Orchidaceae and Allioideae species. The DNA sequences identified will be valuable for future analysis of classical flavonoid genetic loci in Allium crops and will assist the breeding of these important crop species.

  19. The production of reactive oxygen species and the mitochondrial membrane potential are modulated during onion oil-induced cell cycle arrest and apoptosis in A549 cells.

    Science.gov (United States)

    Wu, Xin-jiang; Stahl, Thorsten; Hu, Ying; Kassie, Fekadu; Mersch-Sundermann, Volker

    2006-03-01

    Protective effects of Allium vegetables against cancers have been shown extensively in experimental animals and epidemiologic studies. We investigated cell proliferation and the induction of apoptosis by onion oil extracted from Allium cepa, a widely consumed Allium vegetable, in human lung cancer A549 cells. GC/MS analysis suggested that propyl sulfides but not allyl sulfides are major sulfur-containing constituents of onion oil. Onion oil at 12.5 mg/L significantly induced apoptosis (13% increase of apoptotic cells) as indicated by sub-G1 DNA content. It also caused cell cycle arrest at the G2/M phase; 25 mg/L onion oil increased the percentage of G2/M cells almost 6-fold compared with the dimethyl sulfoxide control. The action of onion oil may occur via a reactive oxygen species-dependent pathway because cell cycle arrest and apoptosis were blocked by the antioxidants N-acetylcysteine and exogenous glutathione. Marked collapse of the mitochondrial membrane potential suggested that dysfunction of the mitochondria may be involved in the oxidative burst and apoptosis induced by onion oil. Expression of phospho-cdc2 and phospho-cyclin B1 were downregulated by onion oil, perhaps accounting for the G2/M arrest. Overall, these results suggest that onion oil may exert chemopreventive action by inducing cell cycle arrest and apoptosis in tumor cells.

  20. Irradiation processing of potatoes, onions and garlic

    International Nuclear Information System (INIS)

    Jan, M.; Starr, A.; Wahid, M.; Ahmad, A.; Khan, I.

    1992-07-01

    In this report, studies have been conducted on irradiation preservation of potatoes, onions and garlic involving different varieties, harvesting season and storage life. As a result of these investigations, optimum conditions were established for each crop. The effect of irradiation and storage on sprouting, rottage and weight loss of potatoes is presented. The sprouting initiated in the unirradiated samples after 2 months reaching 51, 80 and 100% on 4, 5 and 6 months storage respectively. Weight losses were observed in the irradiated and unirradiated samples. (A.B.)

  1. 78 FR 52131 - United States Standards for Grades of Creole Onions

    Science.gov (United States)

    2013-08-22

    ... DEPARTMENT OF AGRICULTURE Agricultural Marketing Service [Doc. Number AMS-FV-13-0018] United States Standards for Grades of Creole Onions AGENCY: Agricultural Marketing Service, USDA. ACTION: Notice..., which were issued under the Agricultural Marketing Act of 1946. The Agricultural Marketing Service (AMS...

  2. COMPARATIVE STUDY CONCERNING THE INFLUENCE OF DIFFERENT HERBICIDE TREATMENT IN ONION CULTURE

    Directory of Open Access Journals (Sweden)

    Ioan OROIAN

    2009-06-01

    Full Text Available A comparative study was performed concerning the action of three herbicides (Pantera 40 CE, Fusilade Super and Agil 100 EC on onion culture. The Amstrong onion hybrid was used on clay - aluviovertic chernosem, with NPK fertilization (N80P80K80 during the preparation of the germinative bed. The unfavorable climatic conditions infl uence the effi cacy of the post-emergent applied herbicides, but signifi cant differences were recorded between variants treated with different products. When Pantera 40 CE was used, phytotoxicity phenomena materialized by temporary discoloration of the plants were not recorded, compared to the results obtained when the other herbicides were used. The use of Pantera 40 CE led to the most important production gain, with 9.8% compared to Fusilade super and 4.8% with Agil 100 EC.

  3. Effect of dietary supplementation with onion (Allium cepa L. on performance, carcass traits and intestinal microflora composition in broiler chickens

    Directory of Open Access Journals (Sweden)

    Majid Goodarzi

    2014-02-01

    Full Text Available Objective: To examine the effect of onion (Allium cepa L. as an antibiotic growth promoter substitute on growth performance, carcass traits, and microflora composition in broiler chickens. Methods: A total of 192 one-day old mixed sex broiler chicks (Ross 308 were weighed and randomly allocated to four treatment groups, each with 4 replicate pens of 12 chicks. The dietary treatments consisted of the basal diet (control, antibiotic (15 mg virginiamycin/kg, and control+10 or 30 g fresh onions bulb/kg diet. Body weights of broilers were determined at Day 1, 21, and 42, feed intake was determined at the same periods, and feed conversion ratio was calculated accordingly. At Day 42, two birds per replicate were slaughtered for determination of carcass and organ weights. The populations of Lactobacilli spp. and Escherichia coli were enumerated in ileum by conventional microbiological techniques using selective agar media. Results: Dietary supplementation of 30 g/kg onion increased final body weight of broilers at 42 d of age compared to that of the other treatments (P<0.05. Birds fed 30 g onion/kg in the diet had the highest feed intake than other treatments at different growth periods (P<0.05. Feed conversion ratio, carcass yield and internal organ weights were not affected by the dietary treatments at Day 42. The Lactobacilli spp. population in birds supplemented with onion at the level of 30 g/ kg significantly was higher than other groups at 42 d of age (P<0.05. The lowest Escherichia coli loads were detected in broilers fed diets containing 15 mg virginiamycin/kg. The Escherichia coli loads significantly decreased in broilers fed diets containing 10 or 30 g onion/kg (P<0.05. Conclusions: In conclusion, the results of the current study indicated that supplementing broiler diet with 30 g onion/kg could induce favorable influences on performance and ileum microflora composition.

  4. Association of Enterobacter cloacae and other bacteria with onion bulb rot in the Columbia Basin of Washington and Oregon, USA

    Science.gov (United States)

    Approximately 1.6 million metric tons of onion bulbs are produced annually in the Pacific Northwest USA. Bulb decay can be a major problem and is caused by a variety of plant pathogens. Onion bulbs exhibiting symptoms of bacterial rot were sampled to determine the causal agents. Enterobacter cloacae...

  5. Tracking movement and temperature selection of larvae of two forensically important blow fly species within a "maggot mass".

    Science.gov (United States)

    Johnson, Aidan P; Wighton, Samuel J; Wallman, James F

    2014-11-01

    The current study responds to the lack of understanding about the temperatures experienced by individual blow fly larvae within "maggot masses." The temperature selection of both aggregating (in a mass) and nonaggregating larvae was compared and their pattern of movement assessed. Infrared imaging determined the temperatures within a mass and in the vicinity of the constituent individual larvae, whose movements were tracked by dyeing their tissues red. Individual Chrysomya rufifacies larvae selected temperatures above 27°C, significantly higher than the temperature selected by Calliphora vicina larvae (24.5°C). However, this same difference was not seen within a mass, with both species selecting temperatures around 28°C. Larval movement in a mass was nonrandom, indicating that larvae actively select their position in a mass. Furthermore, larvae have a strong tendency to select the hottest part of a mass; therefore, maximum mass temperatures might provide a reliable proxy for the actual temperatures experienced by larvae. © 2014 American Academy of Forensic Sciences.

  6. Agronomic performance of onion hybrids in Baraúna, in the semi-arid region of Brazil

    Directory of Open Access Journals (Sweden)

    Renato Leandro Costa Nunes

    Full Text Available In the Brazilian state of Rio Grande do Norte, the significant planting of onions is relatively recent, with producers seeking alternative ways to reduce losses. As a result, it was aimed to evaluate the agronomic performance of onion hybrids in the town of Baraúna in the semi-arid region of Rio Grande do Norte. The experimental design was of randomised blocks with six replications. Treatments consisted of eight hybrids (Cronus F1, Predilata F1, Serena F1, Fortaleza F1, Mata Hari, Luana, Lambada, Don Victor and a control cultivar (IPA 11. The following were evaluated: average plant height; average number of leaves; diameter of the pseudostem; productivity; average bulb weight; rate of survival; leaf-waxiness; cultivar cycle; commercial classification of the bulbs. The Serena F1 and Mata Hari cultivars are recommended for the northeast semi-arid region due to high productivity, early cycle and better bulb classification. The use of these cultivars may help, in the short term, to change cultivation of the onion in the state of Rio Grande do Norte.

  7. Radurization of onions for the improvement of storage life

    International Nuclear Information System (INIS)

    Heins, H.G.

    1974-09-01

    Test results for radiation effects in onions are reported. Low radiation doses of gamma rays may be used and irradiation may take place up to four weeks after harvesting for nearly complete sprout inhibition. Mechanical harvesting has no unfavourable influence. Organoleptic properties remain good. Therefore, radurization on a commercial scale is feasible. A survey is given of the equipment, cost, investment, licensing and admission procedure

  8. Genetic analyses of bolting in bulb onion (Allium cepa L.).

    Science.gov (United States)

    Baldwin, Samantha; Revanna, Roopashree; Pither-Joyce, Meeghan; Shaw, Martin; Wright, Kathryn; Thomson, Susan; Moya, Leire; Lee, Robyn; Macknight, Richard; McCallum, John

    2014-03-01

    We present the first evidence for a QTL conditioning an adaptive trait in bulb onion, and the first linkage and population genetics analyses of candidate genes involved in photoperiod and vernalization physiology. Economic production of bulb onion (Allium cepa L.) requires adaptation to photoperiod and temperature such that a bulb is formed in the first year and a flowering umbel in the second. 'Bolting', or premature flowering before bulb maturation, is an undesirable trait strongly selected against by breeders during adaptation of germplasm. To identify genome regions associated with adaptive traits we conducted linkage mapping and population genetic analyses of candidate genes, and QTL analysis of bolting using a low-density linkage map. We performed tagged amplicon sequencing of ten candidate genes, including the FT-like gene family, in eight diverse populations to identify polymorphisms and seek evidence of differentiation. Low nucleotide diversity and negative estimates of Tajima's D were observed for most genes, consistent with purifying selection. Significant population differentiation was observed only in AcFT2 and AcSOC1. Selective genotyping in a large 'Nasik Red × CUDH2150' F2 family revealed genome regions on chromosomes 1, 3 and 6 associated (LOD > 3) with bolting. Validation genotyping of two F2 families grown in two environments confirmed that a QTL on chromosome 1, which we designate AcBlt1, consistently conditions bolting susceptibility in this cross. The chromosome 3 region, which coincides with a functionally characterised acid invertase, was not associated with bolting in other environments, but showed significant association with bulb sucrose content in this and other mapping pedigrees. These putative QTL and candidate genes were placed on the onion map, enabling future comparative studies of adaptive traits.

  9. Mechanical grading in PGI Tropea red onion post harvest operations

    Directory of Open Access Journals (Sweden)

    Bruno Bernardi

    2013-09-01

    Full Text Available The growing interest expressed by consumers toward food products quality as well as toward their linkage to the territory, has led producers to fit to the continuous rising demand for “typical products”, and to look for new and more efficient production and marketing strategies. An emblematic case is represented by Tropea red onion that, as a typical product, plays an important role in economical and rural development of the territory to which it is linked. The organoleptic features offered by “Tropea Red Onion”, PGI certified (Calabria, have to be associated as well to the quality of services that accompanies its processing. Technology application in post-harvest operations, has certainly contributed to make faster and less tiring all processing tasks. The main problem related to the mechanization of Tropea red onion post-harvest operations lies in the removal of the various layers of the external tunic, making it impossible for optical or electronic grader to achieve this task in a satisfactory way since the sensors are not able yet to separate the “bulb” from its involucre. In this context, the current study aims to assess the productivity of three different machines used for round Tropea red onion grading, and determine their work efficiency. The carried out analysis highlighted the ability of the studied machines to ensure a high work capacity, while maintaining a high level of precision during calibration process. Such precision allows to decrease laborer employment and increase processing chain speed, rising as well the annual use of the machines, allowing consequently processing cost savings. For a more profitable employment of such graders, it is, however, necessary from one hand, to properly form the technicians responsible of processing plants management, and from the other hand, to be able to take advantage of a technical assistance network, able to serve users in a short time.

  10. RESPONSE OF ONION (Allium cepa L.) BULB YIELD TO DAY ...

    African Journals Online (AJOL)

    PROF EKWUEME

    2012-06-17

    Jun 17, 2012 ... to the significance of onion bulbs in Nigeria, research efforts at the National Horticultural ... of Jos, Plateau State, Nigeria. O. A. T. Namo, Department of Plant Science and Technology, University of Jos, Plateau State, Nigeria ... plants to a source of light (two mercury solar light fluorescence bulbs of 150 watts ...

  11. A non-destructive method for estimating onion leaf area

    Directory of Open Access Journals (Sweden)

    Córcoles J.I.

    2015-06-01

    Full Text Available Leaf area is one of the most important parameters for characterizing crop growth and development, and its measurement is useful for examining the effects of agronomic management on crop production. It is related to interception of radiation, photosynthesis, biomass accumulation, transpiration and gas exchange in crop canopies. Several direct and indirect methods have been developed for determining leaf area. The aim of this study is to develop an indirect method, based on the use of a mathematical model, to compute leaf area in an onion crop using non-destructive measurements with the condition that the model must be practical and useful as a Decision Support System tool to improve crop management. A field experiment was conducted in a 4.75 ha commercial onion plot irrigated with a centre pivot system in Aguas Nuevas (Albacete, Spain, during the 2010 irrigation season. To determine onion crop leaf area in the laboratory, the crop was sampled on four occasions between 15 June and 15 September. At each sampling event, eight experimental plots of 1 m2 were used and the leaf area for individual leaves was computed using two indirect methods, one based on the use of an automated infrared imaging system, LI-COR-3100C, and the other using a digital scanner EPSON GT-8000, obtaining several images that were processed using Image J v 1.43 software. A total of 1146 leaves were used. Before measuring the leaf area, 25 parameters related to leaf length and width were determined for each leaf. The combined application of principal components analysis and cluster analysis for grouping leaf parameters was used to reduce the number of variables from 25 to 12. The parameter derived from the product of the total leaf length (L and the leaf diameter at a distance of 25% of the total leaf length (A25 gave the best results for estimating leaf area using a simple linear regression model. The model obtained was useful for computing leaf area using a non

  12. Site specific atomic polarizabilities in endohedral fullerenes and carbon onions

    International Nuclear Information System (INIS)

    Zope, Rajendra R.; Baruah, Tunna; Bhusal, Shusil; Basurto, Luis; Jackson, Koblar

    2015-01-01

    We investigate the polarizability of trimetallic nitride endohedral fullerenes by partitioning the total polarizability into site specific components. This analysis indicates that the polarizability of the endohedral fullerene is essentially due to the outer fullerene cage and has insignificant contribution from the encapsulated unit. Thus, the outer fullerene cages effectively shield the encapsulated clusters and behave like Faraday cages. The polarizability of endohedral fullerenes is slightly smaller than the polarizability of the corresponding bare carbon fullerenes. The application of the site specific polarizabilities to C 60 @C 240 and C 60 @C 180 onions shows that, compared to the polarizability of isolated C 60 fullerene, the encapsulation of the C 60 in C 240 and C 180 fullerenes reduces its polarizability by 75% and 83%, respectively. The differences in the polarizability of C 60 in the two onions is a result of differences in the bonding (intershell electron transfer), fullerene shell relaxations, and intershell separations. The site specific analysis further shows that the outer atoms in a fullerene shell contribute most to the fullerene polarizability

  13. Site specific atomic polarizabilities in endohedral fullerenes and carbon onions

    Science.gov (United States)

    Zope, Rajendra R.; Bhusal, Shusil; Basurto, Luis; Baruah, Tunna; Jackson, Koblar

    2015-08-01

    We investigate the polarizability of trimetallic nitride endohedral fullerenes by partitioning the total polarizability into site specific components. This analysis indicates that the polarizability of the endohedral fullerene is essentially due to the outer fullerene cage and has insignificant contribution from the encapsulated unit. Thus, the outer fullerene cages effectively shield the encapsulated clusters and behave like Faraday cages. The polarizability of endohedral fullerenes is slightly smaller than the polarizability of the corresponding bare carbon fullerenes. The application of the site specific polarizabilities to C60@C240 and C60@C180 onions shows that, compared to the polarizability of isolated C60 fullerene, the encapsulation of the C60 in C240 and C180 fullerenes reduces its polarizability by 75% and 83%, respectively. The differences in the polarizability of C60 in the two onions is a result of differences in the bonding (intershell electron transfer), fullerene shell relaxations, and intershell separations. The site specific analysis further shows that the outer atoms in a fullerene shell contribute most to the fullerene polarizability.

  14. Comparative study of the sensitivities of onion and broad bean root ...

    African Journals Online (AJOL)

    Chemicals which cause chromosomal aberration (CA) in plant cells frequently produce identical CA in cultured animal cells. Plant species however, differ in sensitivities. Onion and broad bean (BB) root meristem cells were compared for sensitivity to chlorpyrifos (CPF), mercury chloride (HgCl2), ethyl methanesulphonate ...

  15. Development of Food Functions and Production Process for Onion Vinegar Using a Two-Stage Continuous-Tank Reactor

    OpenAIRE

    小林, 秀彰; 山口, 文; 富田, 弘毅; 中井, 義昭; 管野, 亨; 小林, 正義; KOBAYASHI, Hideaki; YAMAGUCHI, Kazaru; TOMITA, Koki; NAKAI, Yoshiaki; KANNO, Tohru; KOBAYASHI, Masayoshi

    1998-01-01

    A two-stage continuous-tank reactor was developed to optimize the production of onion vinegar, and the onion vinegar produced was studied to determine its benefits for human health. The ”Silan ring” porous ceramics support was available to immobilize microorganisms, maintain higher mechanical strength and provide a stable rate of alcohol production even at higher dilution rates than 1.2 hr^, without wash-out. The forced cyclic operation of reaction temperature yielded an increase of 25% for ...

  16. Effect of Anoxia on Respiration Rate (Fermentative Index and Ethanol Production of Onion Bulbs (Allium cepa L.

    Directory of Open Access Journals (Sweden)

    N. Benkeblia

    2003-01-01

    Full Text Available The physiological behavior, including carbon dioxide production, fermentative index (FI and ethanolic production of onion bulbs kept under total anoxia (l00% N2 was investigated. During the first 24 hours, carbon dioxide production increased from 0.01 to 1.56 kPa Co2, and the average rate of the increase in CO2; production between 0 and 24 hours was 0.09 kPa/h. The Q10, of the fermentative index was l.9. Ethanol produced by onion bulbs kept under anoxia during 6 hours was temperature dependent, and was 0.563 and 0.760 pmol kg-1h-1 at 10 and 20°C respectively, while at 4°C the quantity produced was not detected. It is concluded that onion seems to be less tolerant to anoxia than other vegetables such as artichoke, cauliflower, tomato, potato and asparagus.

  17. Peeling the Onion: How to Promote Pharmaceutical Innovation and Access to Medicine

    DEFF Research Database (Denmark)

    Minssen, Timo

    2015-01-01

    As mentioned in my earlier blog post, I decided to conclude this year by publishing a introductory speech that I gave on April 14th, 2015 at the 2015 Broad Institute Innovation & Intellectual Property Symposium. The speech was part of the session “Bringing Therapies to the Patients” and introduced...... in pharmaceutical innovation, sometimes feels like – to lend the words of the late German Nobel Prize winner Günter Grass – “peeling an onion:” First you are amazed by the number of overlapping layers of interacting laws and regulations and the many aspects of the debate. However, once you peeled long enough you...... to be found outside the world of patent law. [.....] Continued at: http://blogs.harvard.edu/billofhealth/2015/12/31/peeling-the-onion-how-to-promote-pharmaceutical-innovation-and-access-to-medicine-part-i/...

  18. Determining the potential link between irrigation water quality and the microbiological quality of onions by phenotypic and genotypic characterization of Escherichia coli isolates.

    Science.gov (United States)

    du Plessis, Erika M; Duvenage, Francois; Korsten, Lise

    2015-04-01

    The potential transfer of human pathogenic bacteria present in irrigation water onto fresh produce was investigated, because surface water sources used for irrigation purposes in South Africa have increasingly been reported to be contaminated with enteric bacterial pathogens. A microbiological analysis was performed of a selected river in Limpopo Province, South Africa, that is often contaminated with raw sewage from municipal sewage works and overhead irrigated onions produced on a commercial farm. Counts of Escherichia coli, coliforms, aerobic bacteria, fungi, and yeasts and the prevalence of E. coli O157:H7, Salmonella, and Listeria monocytogenes were determined. Identities of bacterial isolates from irrigation water and onions were confirmed using matrix-assisted laser desorption ionization-time of flight mass spectrometry, PCR, and biochemical tests. To establish a potential link between the microbiological quality of the irrigation source and the onions, the E. coli isolates from both were subjected to antibiotic resistance, virulence gene, and enterobacterial repetitive intergenic consensus PCR analyses. River water E. coli counts exceeded South African Department of Water Affairs and World Health Organization irrigation water guidelines. Counts of aerobic bacteria, coliforms, fungi, and yeasts of onions from the market were acceptable according to Department of Health Directorate, Food Control, South Africa, microbiological guidelines for ready-to-eat fresh fruits and vegetables. E. coli O157:H7, Salmonella, and L. monocytogenes were not detected in onions, whereas only Salmonella was detected in 22% of water samples. Matrix-assisted laser desorption ionization-time of flight mass spectrometry and PCR identification of E. coli isolates from water and onions correlated. Of the 45 E. coli isolates from water and onions, 42.2% were resistant to multiple antibiotics. Virulence genes eae, stx1, and stx2 were detected in 2.2, 6.6, and 2.2% of the E. coli isolates

  19. Food irradiation in the control of storage and handling losses in the Philippine onions and garlic

    International Nuclear Information System (INIS)

    Lustre, A.O.; Dianco, A.M.; Vito, F.R.; Manalastas, Z.L.

    1996-01-01

    Information will be provided on the nature and magnitude of the control of postharvest losses in onions and garlic from the application of irradiation. Control of losses is measured in storage and during post storage marketing of the commodities. Information will also be presented on market tests and survey of consumer reaction to irradiated onions. The benefits of irradiation will be discussed in relation to the need to reduce postharvest losses in the food supply and to address consumer concerns over the safety of food due to the use of chemicals. (author)

  20. Synergic effect of Citric Acid and Red Onion skin extract on the ...

    African Journals Online (AJOL)

    Michael Horsfall

    In the food industry, various synthetic antioxidants are often used to ... among beverages. (Nicoletta et al, 2003). ... was obtained from the Choba market. The onion skin ... and shaken to mix the extract and vegetable oil. The treated and control ...

  1. Molecular diversity of arbuscular mycorrhizal fungi in onion roots from organic and conventional farming systems in the Netherlands.

    Science.gov (United States)

    Galván, Guillermo A; Parádi, István; Burger, Karin; Baar, Jacqueline; Kuyper, Thomas W; Scholten, Olga E; Kik, Chris

    2009-06-01

    Diversity and colonization levels of naturally occurring arbuscular mycorrhizal fungi (AMF) in onion roots were studied to compare organic and conventional farming systems in the Netherlands. In 2004, 20 onion fields were sampled in a balanced survey between farming systems and between two regions, namely, Zeeland and Flevoland. In 2005, nine conventional and ten organic fields were additionally surveyed in Flevoland. AMF phylotypes were identified by rDNA sequencing. All plants were colonized, with 60% for arbuscular colonization and 84% for hyphal colonization as grand means. In Zeeland, onion roots from organic fields had higher fractional colonization levels than those from conventional fields. Onion yields in conventional farming were positively correlated with colonization level. Overall, 14 AMF phylotypes were identified. The number of phylotypes per field ranged from one to six. Two phylotypes associated with the Glomus mosseae-coronatum and the G. caledonium-geosporum species complexes were the most abundant, whereas other phylotypes were infrequently found. Organic and conventional farming systems had similar number of phylotypes per field and Shannon diversity indices. A few organic and conventional fields had larger number of phylotypes, including phylotypes associated with the genera Glomus-B, Archaeospora, and Paraglomus. This suggests that farming systems as such did not influence AMF diversity, but rather specific environmental conditions or agricultural practices.

  2. Influence of Cultivars and Seed Thermal Treatment on the Development of Fungal Pathogens in Carrot and Onion Plants

    Directory of Open Access Journals (Sweden)

    Martin Koudela

    2016-01-01

    Full Text Available Carrot and onion are vegetables representing an important segment of fresh market. They suffer from serious fungal diseases that can inflict great damage on crops, i.e. alternaria leaf blight, peronospora downy mildew, and botrytis neck rot. The resistance of selected carrot and onion cultivars important for the production of vegetables in the Czech Republic was tested by exposure to targeted infection by the above fungal pathogens. The exposure of eleven carrot cultivars to spores of Alternaria dauci showed that the most resistant and sensitive cultivars were Katrin, Cortina F1, Afalon F1 and Favorit, Tinga, Berlika F1, respectively. A targeted infection of onion cultivars with Botrytis aclada clustered them into three groups: Amfora F1, Bolero, Tosca, Triumf F1 (strong resistance, Avalon, Grenada (medium resistance, Alice, Karmen, Všetana (low resistance. Similar groups were distinguished also after the infection with Peronospora destructor: Avalon, Bolero, Tosca (strong resistance, Alice, Amfora F1, Grenada, Karmen, Triumf F1 (medium resistance,Všetana (low resistance. Hot water treatment of carrot seeds applied after the inoculation with A. dauci decreased the development of the infection 1.3-2.3-fold, whereas the protective effect observed with onion seeds against the infection by P. destructor and B. aclada was lower.

  3. An Assessment of Urea-Formaldehyde Fertilizer on the Diversity of Bacterial Communities in Onion and Sugar Beet

    OpenAIRE

    Ikeda, Seishi; Suzuki, Keijiro; Kawahara, Makoto; Noshiro, Masao; Takahashi, Naokazu

    2014-01-01

    The impact of a urea-formaldehyde (UF) fertilizer on bacterial diversity in onion bulbs and main roots of sugar beet were examined using a 16S rRNA gene clone library. The UF fertilizer markedly increased bacterial diversity in both plants. The results of principal coordinates analysis (PCoA) revealed that nearly 30% of the variance observed in bacterial diversity in both the onion and sugar beet was attributed to the fertilization conditions and also that the community structures in both pla...

  4. Drying kinetics and quality aspects during heat pump drying of onion (Allium cepa L.

    Directory of Open Access Journals (Sweden)

    Nihar Ranjan Sahoo

    2012-10-01

    Full Text Available Normal 0 false false false MicrosoftInternetExplorer4 A prototype heat pump dryer has been developed for drying of fruits and vegetables at low temperature and relative humidity to maintain the quality of dried product. Onions, of Nasik red variety were peeled, trimmed and sliced to 2 mm thickness. The onion slices were dried in the heat pump dryer at 35ºC (32 % R.H., 40ºC (26 % R.H., 45ºC (19 % R.H. and 50ºC (15 % R.H.. Samples were also dried in a hot air dryer at 50ºC (52 % R.H. for comparison. The drying rate increased with increase in drying air temperature, associated with reduced R.H., in the heat pump dryer. Drying took place mainly under the falling rate period. The Page equation, resulting in a higher coefficient of determination and lower root mean square error, better described the thin-layer drying of onion slices than the Henderson and Pabis equation. Heat pump drying took less drying time of 360 min and yielded better quality dried product, with higher retention of ascorbic acid and pyruvic acid and lower colour change, as compared to a hot air dryer at the same drying air temperature of 50ºC.

  5. Effect of gamma radiation on the viable cell count and some other quality characteristics of dried onions

    International Nuclear Information System (INIS)

    Farkas, J.; El-Nawawy, A.S.

    1973-01-01

    The results of experiments carried out with onion powder and flakes of Egyptian origin show a radiation treatment of 0.1-0.2 Mrad sufficient to reduce the microbial contamination of dried onions by one order of magnitude. To achieve a 99% reduction, satisfactory from the practical point of view, treatment with 0.5-0.6 Mrad seems necessary. With increasing radiation doses the characteristic smell of dried onions diminished and a caramel-like off-odour appeared and the colour of the dried products darkened. These changes become significant only above treatment with 0.8 Mrad. The differences between radiation treated and untreated samples both packed in Cellothene pouches were either eliminated (odour) or diminished (colour) during 6 weeks storage following irradiation. The results obtained by the chemical oxygen demand method (COD) based on bichromate oxidation of water soluble volatile reducing substances, were not in accord with the organoleptically observable changes of odour. (F.J.)

  6. Pelargonic acid for weed control in organic Vidalia sweet onion production

    Science.gov (United States)

    Cultivation using a tine weeder is a proven means to manage weeds in organic Vidalia® sweet onion production. If the initial cultivation is delayed, emerged weeds are not controlled by the tine weeder. In these cases, herbicides derived from natural products could be used to control the emerged we...

  7. Feasibility of radiation preservation of potatoes and onions in Pakistan

    International Nuclear Information System (INIS)

    Khan, Ismail; Muhammad, Amir.

    1975-01-01

    The storage problem of potatoes and onions in Pakistan has been explained. The feasibility of preserving these vegetables by radiation and the cost and economics of the technology is examined. It has been concluded that radiation with subsequent storage at 15 0 -20 0 C will not only reduce the spoilage losses considerably but also give great benefit to the producer

  8. A new acetonitrile-free mobile phase method for LC-ELSD quantification of fructooligosaccharides in onion (Allium cepa L.).

    Science.gov (United States)

    Downes, Katherine; Terry, Leon A

    2010-06-30

    Onion soluble non-structural carbohydrates consist of fructose, glucose and sucrose plus fructooligosaccharides (FOS) with degrees of polymerisation (DP) in the range of 3-19. In onion, sugars and FOS are typically separated using liquid chromatography (LC) with acetonitrile (ACN) as a mobile phase. In recent times, however, the production of ACN has diminished due, in part, to the current worldwide economic recession. A study was therefore undertaken, to find an alternative LC method to quantify sugars and FOS from onion without the need for ACN. Two mobile phases were compared; the first taken from a paper by Vågen and Slimestad (2008) using ACN mobile phase, the second, a newly reported method using ethanol (EtOH). The EtOH mobile phase eluted similar concentrations of all FOS compared to the ACN mobile phase. In addition, limit of detection, limit of quantification and relative standard deviation values were sufficiently and consistently lower for all FOS using the EtOH mobile phase. The drawback of the EtOH mobile phase was mainly the inability to separate all individual sugar peaks, yet FOS could be successfully separated. However, using the same onion extract, a previously established LC method based on an isocratic water mobile phase could be used in a second run to separate sugars. Although the ACN mobile phase method is more convenient, in the current economic climate a method based on inexpensive and plentiful ethanol is a valid alternative and could potentially be applied to other fresh produce types. In addition to the mobile phase solvent, the effect of extraction solvents on sugar and FOS concentration was also investigated. EtOH is still widely used to extract sugars from onion although previous literature has concluded that MeOH is a superior solvent. For this reason, an EtOH-based extraction method was compared with a MeOH-based method to extract both sugars and FOS. The MeOH-based extraction method was more efficacious at extracting sugars and

  9. Integrated systems of weed management in organic transplated vidalia sweet onion production

    Science.gov (United States)

    Field experiments were conducted from 2008 through 2010 near Lyons, GA to develop integrated weed management systems for organic Vidalia® sweet onion production. Treatments were a factorial arrangement of summer solarization, cultivation with a tine weeder, and a clove oil herbicide. Plots were so...

  10. Effective onion leaf fleck management and variability of storage pathogens

    Directory of Open Access Journals (Sweden)

    Rasiukevičiūtė Neringa

    2016-01-01

    Full Text Available Botrytis spp. cause several diseases in Allium crops and depending on meteorological conditions economic losses can exceed 50%. Forecasting models improve plant protection and sometimes reduce consumption of fungicides, because applications are made precisely during the favourable periods for disease development. Our aim was to evaluate the iMETOS®sm B. cinerea forecasting model as an effective onion leaf fleck management system and estimate the variability of onion bulb pathogens during storage. Assessment of forecasting model data showed that favourable conditions for leaf fleck development arise in July, but greatly depend on that year’s meteorological conditions. During an experimental year the first sprayings with fungicides were applied as forecasted from the model, which resulted in application 19, 6 and 23 days earlier than conventional treatment application times. In 2012-2014 iMETOS®sm treatment yield increased by 3.51 t ha-1, 3.87 t ha-1 and3.40 t ha-1 relative to the control. During storage most frequent injuries were fungal (44% and bacterial (41%, followed by insects (7% and physiological (9%. The highest prevalence of injuries was detected after 2 months of storage.

  11. Site specific atomic polarizabilities in endohedral fullerenes and carbon onions

    Energy Technology Data Exchange (ETDEWEB)

    Zope, Rajendra R., E-mail: rzope@utep.edu; Baruah, Tunna [Department of Physics, The University of Texas at El Paso, El Paso, Texas 79958 (United States); Computational Science Program, The University of Texas at El Paso, El Paso, Texas 79958 (United States); Bhusal, Shusil; Basurto, Luis [Department of Physics, The University of Texas at El Paso, El Paso, Texas 79958 (United States); Jackson, Koblar [Physics Department and Science of Advanced Materials Ph.D. Program, Central Michigan University, Mt. Pleasant, Michigan 48859 (United States)

    2015-08-28

    We investigate the polarizability of trimetallic nitride endohedral fullerenes by partitioning the total polarizability into site specific components. This analysis indicates that the polarizability of the endohedral fullerene is essentially due to the outer fullerene cage and has insignificant contribution from the encapsulated unit. Thus, the outer fullerene cages effectively shield the encapsulated clusters and behave like Faraday cages. The polarizability of endohedral fullerenes is slightly smaller than the polarizability of the corresponding bare carbon fullerenes. The application of the site specific polarizabilities to C{sub 60}@C{sub 240} and C{sub 60}@C{sub 180} onions shows that, compared to the polarizability of isolated C{sub 60} fullerene, the encapsulation of the C{sub 60} in C{sub 240} and C{sub 180} fullerenes reduces its polarizability by 75% and 83%, respectively. The differences in the polarizability of C{sub 60} in the two onions is a result of differences in the bonding (intershell electron transfer), fullerene shell relaxations, and intershell separations. The site specific analysis further shows that the outer atoms in a fullerene shell contribute most to the fullerene polarizability.

  12. The influence of irradiation and packaging on the keeping quality of prepacked cut endive, chicory and onions

    International Nuclear Information System (INIS)

    Langerak, D.Is.

    1975-01-01

    The experiments with endive and chicory showed that by washing immediatedly after cutting, pink-discolouration decreased. During storage, however, this discolouration increased again. Discolouration was partially prevented by packaging in nonperforated polythene foil of 0.02 mm thereby decreasing the O 2 and increasing the CO 2 content in the bags. A difficulty in using non-perforated bags was that in non-irradiated products, fermentation took place caused by a high microbial count /10 8 g -1 /. In irradiated samples no fermentation occurred probably due to the decreased microbial count. Cut onions had to be packed in perforated polythene bags because strong gas /CO 2 / development took place in non-perforated bags at temperatures above 10 0 C. The initial total viable count of endive, chicory and onions varied from 10 3 to 10 7 g -1 . Irradiation with doses above 50 krad gave a reduction of this count of 3-6 log cycles through which the keeping time was lengthened by about 100% at 10-20 0 C. The Enterobacteriaceae were mainly eliminated by irradiation doses higher than 100 krad. The optimal dose for chicory, onions and endive was found to be 50, 75, and 100 krad, respectively. Higher doses intensified discolouration and influenced unfavourably the flavour. Cooking experiments with cut onions packed in perforated bags showed that the non-irradiated product discoloured quickly into brown after cooking. By irradiation with 75-100 krad this discolouration was prevented. (F.J.)

  13. ESR and microbiological studies on gamma radiation processed dehydrated onion powder

    International Nuclear Information System (INIS)

    Khedkar, K.C.; Kumbhare, M.N.; Sharma, G.; Lavale, D.S.; Kadam, R.M.; Bhide, M.K.; Sastry, M.D.

    2001-01-01

    Paper describes the potential of ESR technique for detection of free radicals produced in the irradiated onion powder. Experiments were carried out to evaluate the effect of light, moisture and time on the stability of free radicals produced by gamma radiation. Some microbiological studies were also carried out on unprocessed and radiation-processed product. (author)

  14. Protoplasmic Swelling as a Symptom of Freezing Injury in Onion Bulb Cells 1

    Science.gov (United States)

    Arora, Rajeev; Palta, Jiwan P.

    1986-01-01

    Freezing injury, in onion bulb tissue, is known to cause enhanced K+ efflux accompanied by a small but significant loss of Ca2+ following incipient freezing injury and swelling of protoplasm during the postthaw secondary injury. The protoplasmic swelling of the cell is thought to be caused by the passive influx of extracellular K+ into the cell followed by water uptake. Using outer epidermal layer of unfrozen onion bulb scales (Allium cepa L. cv Big Red), we were able to stimulate the irreversible freezing injury symptoms, by bathing epidermal cells in 50 millimolar KCl. These symptoms were prevented by adding 20 millimolar CaCl2 to the extracellular KCl solution. Our results provide evidence that loss of cellular Ca2+ plays an important role in the initiation and the progression of freezing injury. Images Fig. 1 PMID:16665083

  15. Broadcast application of scythe for broadleaf weed control in spring-transplanted onions

    Science.gov (United States)

    Producers using organic methods for onion production need organic herbicides that will effectively provide post-emergent weed control. In 2008, a second year of field research was conducted to determine the effect of broadcast over-the-top application of Scythe (57% pelargonic acid) on weed control...

  16. Effects of onion extract on endogenous vascular H2S and adrenomedulin in rat atherosclerosis.

    Science.gov (United States)

    Li, Wei; Tang, Chaoshu; Jin, Hongfang; Du, Junbao

    2011-09-01

    This study aimed to explore the effect of onion extract on endogenous hydrogen sulfide (H2S) and adrenomedulin (ADM) and on atherosclerotic progression in rats with atherosclerosis (AS). Male Sprague-Dawley rats were randomly divided into control, AS and AS+onion groups. Ultrastructure of aorta and atherosclerotic lesions both in aorta and in coronary artery were detected. Plasma and aortic H2S were detected by using a sulfide- sensitive electrode. Plasma and aortic ADM was determined with radioimmunoassay. Cystathionine-γ-lyase (CSE), calcitonin receptor-like receptor (CRLR), receptor activity-modifying protein (RAMP1, RAMP2 and RAMP3) mRNA expressions were analysed. Glutathione peroxidase (GSH-PX), superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide (NO) and NO synthase (NOS) contents in plasma, SOD1, SOD2 and ICAM-1 expressions in aorta were detected. Rats in the AS group showed marked atherosclerotic lesions both in aorta and in coronary artery but decreased aortic H2S production. Decreased plasma and aortic ADM content, but increased levels of aortic CRLR, RAMP2 and RAMP3 mRNAs were observed. Plasma GSH-PX and SOD were reduced but MDA elevated. Plasma ICAM-1 and NO contents and iNOS activity were increased. Onion extract, however, lessened atherosclerotic lesions and increased endogenous aortic H2S production, but decreased plasma ADM content, aortic ADM content and aortic CRLR, RAMP2 and RAMP3 mRNAs. In addition, it increased plasma GSH-PX level and SOD activities but reduced MDA; it decreased inflammatory response but increased plasma eNOS activity and NO content. Onion extract exerted a marked antiatherogenic effect in association with the up-regulation of the endogenous CSE/H2S pathway but down-regulation of the ADM/CRLR family in atherosclerotic rats.

  17. Dual Inoculation with Mycorrhizal and Saprotrophic Fungi Applicable in Sustainable Cultivation Improves the Yield and Nutritive Value of Onion

    OpenAIRE

    Albrechtova, Jana; Latr, Ales; Nedorost, Ludovit; Pokluda, Robert; Posta, Katalin; Vosatka, Miroslav

    2012-01-01

    The aim of this paper was to test the use of dual microbial inoculation with mycorrhizal and saprotrophic fungi in onion cultivation to enhance yield while maintaining or improving the nutritional quality of onion bulbs. Treatments were two-factorial: (1) arbuscular mycorrhizal fungi (AMF): the mix corresponding to fungal part of commercial product Symbivit (Glomus etunicatum, G. microaggregatum, G. intraradices, G. claroideum, G. mosseae, and G. geosporum) (M1) or the single-fungus inoculum ...

  18. Intoxicação experimental por cebola, Allium cepa (Liliaceae, em gatos Experimental onion Allium cepa (Liliaceae poisoning in cats

    Directory of Open Access Journals (Sweden)

    Rafael A. Fighera

    2002-04-01

    Full Text Available A intoxicação por cebola é relatada em várias espécies animais em muitas partes do mundo. O princípio tóxico (n-propil dissulfito presente na cebola causa a transformação da hemoglobina em metemoglobina. Para estudar os achados laboratoriais, de necropsia e histopatológicos da intoxicação por cebola em gatos, cinco gatos de quatro meses de idade receberam cada um uma dose única de 10g/kg de cebola desidratada por via oral. Um outro gato de mesma idade não recebeu a refeição com cebola e serviu como controle. Todos os cinco gatos desenvolveram sinais clínicos da toxicose; um deles morreu dentro de 24 horas após a ingestão da cebola. Os sinais clínicos incluíram apatia, taquicardia, taquipnéia e cianose. Os achados laboratoriais se caracterizavam por anemia hemolítica associada a corpúsculos de Heinz e metemoglobinemia. Os principais achados de necropsia foram esplenomegalia e sangue de cor marrom. Os achados histopatológicos foram hemossiderose e hematopoese extramedular no baço e fígado.Onion poisoning is reported worlwide in several animal species. The toxic principle (n-propyl dissulfide present in onions causes the transformation of hemoglobin in methemoglobin. In order to study the laboratory, gross and histopathological findings in onion poisoning in cats, five 4-month-old cats were fed a single dose of 10g/kg of dried-onion each. Another cat of the same age did not receive the onion meal and served as control. All five cats developed clinical signs of the toxicosis; one of them died within 24 hours of the ingestion of the onion meal. Clinical signs included apathy, tachycardia, tachypnea, and cyanosis. Laboratory findings included hemolytic anemia associated with Heinz bodies and methemoglobinemia. Main necropsy findings were splenomegaly and brown discoloration of blood. Histopathological findings included splenic and hepatic hemosiderosis and multifocal extramedullary hematopoiesis.

  19. Effects of onion (Allium cepa L.) and garlic (Allium sativum L.) essential oils on the Aspergillus versicolor growth and sterigmatocystin production.

    Science.gov (United States)

    Kocić-Tanackov, Sunčica; Dimić, Gordana; Lević, Jelena; Tanackov, Ilija; Tepić, Aleksandra; Vujičić, Biserka; Gvozdanović-Varga, Jelica

    2012-05-01

    In the present study the effects of individual and combined essential oils (EOs) extracted from onion (Allium cepa L.) bulb and garlic (Allium sativum L.) clove on the growth of Aspergillus versicolor and sterigmatocystin (STC) production were investigated. The EOs obtained by hydrodistillation were analyzed by GC/MS. Twenty one compounds were identified in onion EO. The major components were: dimethyl-trisulfide (16.64%), methyl-propyl-trisulfide (14.21%), dietil-1,2,4-tritiolan (3R,5S-, 3S,5S- and 3R,5R- isomers) (13.71%), methyl-(1-propenyl)-disulfide (13.14%), and methyl-(1-propenyl)-trisulfide (13.02%). The major components of garlic EO were diallyl-trisulfide (33.55%), and diallyl-disulfide (28.05%). The mycelial growth and the STC production were recorded after 7, 14, and 21 d of the A. versicolor growth in Yeast extract sucrose (YES) broth containing different EOs concentrations. Compared to the garlic EO, the onion EO showed a stronger inhibitory effect on the A. versicolor mycelial growth and STC production. After a 21-d incubation of fungi 0.05 and 0.11 μg/mL of onion EO and 0.11 μg/mL of garlic EO completely inhibited the A. versicolor mycelial growth and mycotoxins biosynthesis. The combination of EOs of onion (75%) and garlic (25%) had a synergistic effect on growth inhibition of A. versicolor and STC production. © 2012 Institute of Food Technologists®

  20. Effect of leek and onion on processing and quality characteristics of Greek traditional sausages.

    Science.gov (United States)

    Fista, G A; Bloukas, J G; Siomos, A S

    2004-10-01

    The objective of this study was to investigate the effect of leek and onion on processing and quality characteristics of sausages and select the most appropriate, to determine the optimum level of selected vegetable and to improve its effectiveness on quality characteristics of sausages, in comparison to the addition of nitrites (100 ppm), by using a starter culture of Staphylococcus carnosus and ascorbate. The nitrate content of leek ranged from 213 to 255 ppm and that of onion was 79 ppm. Sausages produced with leek had higher (pnitrite content (1.3-2.1 ppm) and a (∗)(+) values and higher scores for sensory attributes than sausages with onion. Sausages made with the total leek plant had the highest score for overall acceptability. The higher the leek level the higher the nitrate and nitrite content of sausages and the lower the redness, a (∗)(+). Sausages with 240 g of leek/kg had the highest (pnitrite content, higher weight losses and lower pH values after the 3rd day of storage, compared to sausages produced with the addition of sodium nitrite. The addition of starter culture and ascorbic acid improved the redness (a (∗)) of sausages and reduced the 2-thiobarbituric acid value. Sausages with leek, starter culture and ascorbic acid had the highest score for odour and taste and overall acceptability.

  1. Arsenic and trace elements in soil, water, grapevine and onion in Jáchal, Argentina.

    Science.gov (United States)

    Funes Pinter, Iván; Salomon, M Victoria; Gil, Raúl; Mastrantonio, Leandro; Bottini, Rubén; Piccoli, Patricia

    2018-02-15

    Contamination by trace elements (TE) is an increasing concern worldwide. In some areas, crop production could be limited by the presence of metals and metalloids, so it is important to determine their concentrations and mobility. The region of Jáchal, province of San Juan, Argentina, has good growing conditions for onion and grapevine production, but their quality and yield are affected by high TE concentration in soils and water. Soils, water, grapevine and onion were sampled and TE content determined. In soils elevated As, B, Cr, Hg, and Tl concentrations were detected (506±46, 149±3, 2714±217, 16±7, and 12±3μgg -1 , respectively, for maximum values measured), and physicochemical properties of the soil promotes these elements mobility. Water samples had high As, B, Cr, and Fe concentrations (1438±400, 10,871±471, 11,516±2363, and 3071±257μgL -1 , respectively, for maximum values measured) while in onion bulbs and grapevine berries, As, Cr, Cu, and Fe (92±7 and 171±20, 1412±18 and 2965±32, 17±3 and 126±88, and 418±204 and 377±213μgg -1 , respectively, for maximum values measured) exceeded the limits for food consumption established by Argentinian law. Correlation analyses indicated that: i) there is a common source of TE in this area, ii) each elements concentration in plants is associated with different soil variables and different soils depths, and iii) the lack of correlation between soil and water indicates that concentration in water is not constant over the time and/or there exists a differential accumulation of elements in soils depending on their own properties. Data obtained demonstrate very high concentration of TE in soil, grapevines, and onion plants in Jáchal region, and different remediation techniques are necessary to stabilize and minimize the bioavailability of these elements. Copyright © 2017 Elsevier B.V. All rights reserved.

  2. The efficacy and safety of a proprietary onion-pumpkin extract (OPtain120 on blood pressure: an open-label study

    Directory of Open Access Journals (Sweden)

    Orie Yoshinari

    2015-06-01

    Full Text Available Background: Nutraceuticals and functional foods are increasingly being used to help manage hypertension. Treatment with either pumpkin or onion can significantly lower systolic and diastolic blood pressure in animal studies. Traditionally, pumpkin has been used to support healthy blood pressure, glucose tolerance and lipid levels. Onion contains high levels of flavonoids, including quercetin, which decreases blood pressure and promotes restoration of healthy endothelial function. However, human trials on these food sources are limited, and the combined effects of pumpkin and onion have not been examined yet. Objective: We performed an open-label clinical study to evaluate the effects of a proprietary onion-pumpkin extract (OPtain120 on systolic and diastolic blood pressure. Methods: Healthy adults with systolic blood pressure (SBP and diastolic blood pressure (DBP in the elevated range of 140-159 and 80-90 mmHg, respectively, were enrolled in this study. Subjects consumed one capsule of onion-pumpkin extract twice daily for 12 weeks. Daily Home Blood Pressure Measurement (HBPM was taken upon waking and before bed. Office Blood Pressure Measurement (OBPM was taken in-clinic at Week 0, 6, and 12. Results: 52 subjects were screened and 12 were enrolled in the study, with a total of 10 subjects completing the study. Systolic HBPM taken before bed demonstrated a statistically significant reduction from baseline (147.23 mmHg to Week 12 (138.14 mmHg, representing a reduction of 9.09 mmHg (6.17%, p=0.021. Diastolic HBPM taken before bed demonstrated a decrease of 4.06 mmHg (4.46%, p=0.085, a significant reduction from baseline (91.07 mmHg at Week 12 (87.02 mmHg. Non-statistically significant reductions were seen in the early morning Systolic (3.14% and Diastolic (2.57% HBPM and in the Systolic (1.36% OBPM. Conclusion: OPtain120 was safely consumed over a 12-week period. OPtain120 appears to be effective in lowering Systolic Blood Pressure at bedtime in

  3. Determination of total As in onion plants growing in contaminated substrates by total reflection X-ray fluorescence

    International Nuclear Information System (INIS)

    Lue-Meru Marco Parra

    2011-01-01

    The onion (Allium cepa L.) is one of the most important cultivars in the world and its production level occupies the second place in Venezuela. It becomes important to develop analytical procedures for arsenic determination and to study the effect of this element on the cultures, as well the absorption, transport and translocation processes. A TXRF method for As determination in onions was developed. Two treatments were applied to the onion plants, As contaminated and control. The contaminant was added to the plants to an amount of 100 μg, in a single time 3 weeks after the transplant of plantlets. The green leaves bulbs, and roots together with the stems were separated 45 days after transplant and analyzed by TXRF and HG-AAS for total Arsenic determination. A good agreement was found between these two techniques, demonstrating the accuracy of the TXRF procedure. It was found that the highest concentration corresponded to the root and stems (37 ± 31 μg g -1 ), followed by the bulbs (11 ± 7 μg g -1 ), being the smallest level found in the green leaves (4 ± 3 μg g -1 ). At low As contamination levels of 0.25 μg g -1 , a risk for translocation of the toxic element to the edible parts of the onion plants exists. At this level the normal development of the plant is not affected, being the only exception the root length, which is significantly higher in the contaminated treatment. (author)

  4. Dual inoculation with mycorrhizal and saprotrophic fungi applicable in sustainable cultivation improves the yield and nutritive value of onion.

    Science.gov (United States)

    Albrechtova, Jana; Latr, Ales; Nedorost, Ludovit; Pokluda, Robert; Posta, Katalin; Vosatka, Miroslav

    2012-01-01

    The aim of this paper was to test the use of dual microbial inoculation with mycorrhizal and saprotrophic fungi in onion cultivation to enhance yield while maintaining or improving the nutritional quality of onion bulbs. Treatments were two-factorial: (1) arbuscular mycorrhizal fungi (AMF): the mix corresponding to fungal part of commercial product Symbivit (Glomus etunicatum, G. microaggregatum, G. intraradices, G. claroideum, G. mosseae, and G. geosporum) (M1) or the single-fungus inoculum of G. intraradices BEG140 (M2) and (2) bark chips preinoculated with saprotrophic fungi (mix of Gymnopilus sp., Agrocybe praecox, and Marasmius androsaceus) (S). The growth response of onion was the highest for the M1 mix treatment, reaching nearly 100% increase in bulb fresh weight. The effectiveness of dual inoculation was proved by more than 50% increase. We observed a strong correlation (r = 0.83) between the growth response of onion bulbs and AM colonization. All inoculation treatments but the single-fungus one enhanced significantly the total antioxidant capacity of bulb biomass, was the highest values being found for M1, S + M1, and S + M2. We observed some induced enhancement of the contents of mineral elements in bulb tissue (Mg and K contents for the M2 and M2, S, and S + M2 treatments, resp.).

  5. Dual Inoculation with Mycorrhizal and Saprotrophic Fungi Applicable in Sustainable Cultivation Improves the Yield and Nutritive Value of Onion

    Directory of Open Access Journals (Sweden)

    Jana Albrechtova

    2012-01-01

    Full Text Available The aim of this paper was to test the use of dual microbial inoculation with mycorrhizal and saprotrophic fungi in onion cultivation to enhance yield while maintaining or improving the nutritional quality of onion bulbs. Treatments were two-factorial: (1 arbuscular mycorrhizal fungi (AMF: the mix corresponding to fungal part of commercial product Symbivit (Glomus etunicatum, G. microaggregatum, G. intraradices, G. claroideum, G. mosseae, and G. geosporum (M1 or the single-fungus inoculum of G. intraradices BEG140 (M2 and (2 bark chips preinoculated with saprotrophic fungi (mix of Gymnopilus sp., Agrocybe praecox, and Marasmius androsaceus (S. The growth response of onion was the highest for the M1 mix treatment, reaching nearly 100% increase in bulb fresh weight. The effectiveness of dual inoculation was proved by more than 50% increase. We observed a strong correlation (r=0.83 between the growth response of onion bulbs and AM colonization. All inoculation treatments but the single-fungus one enhanced significantly the total antioxidant capacity of bulb biomass, was the highest values being found for M1, S + M1, and S + M2. We observed some induced enhancement of the contents of mineral elements in bulb tissue (Mg and K contents for the M2 and M2, S, and S+M2 treatments, resp..

  6. Effect of endomycorrhizae on the bioavailability of bound 14C residues to onion plants from an organic soil treated with [14C]fonofos

    International Nuclear Information System (INIS)

    Nelson, S.D.; Khan, S.U.

    1990-01-01

    Uptake of bound 14 C residues from an organic soil treated with radiolabeled fonofos (O-ethyl S-phenyl ethylphosphonodithioate) by selected Glomus endomycorrhiza and onion roots was studied. The hyphae of endomycorrhizal associations were capable of removing 14 C residues from the soil and transporting them to onion plants. Bioavailability of soil-bound 14 C residues, as measured by 14 C residue content in onion, was increased 32 and 40% over that of nonmycorrhizal plants by hyphae of Glomus intraradices and Glomus vesiculiferium, respectively. The data suggest that under field conditions endomycorrhizal infection may greatly increase the bioavailability of soil-bound pesticide residues to plants

  7. Effect of diets fortified with tomatoes or onions with variable quercetin-glycoside content on azoxymethane-induced aberrant crypt foci in the colon of rats

    NARCIS (Netherlands)

    Femia, A.P.; Caderni, G.; Ianni, M.; Salvadori, M.; Schijlen, E.G.W.M.; Collins, G.; Bovy, A.G.; Dolara, P.

    2003-01-01

    Background: Onion and tomato are vegetables widely consumed by humans and epidemiological studies show an inverse association between vegetable consumption and colon cancer risk; however, the effect on colon cancer of diets containing high levels of vegetables like onion and tomato are not clear.

  8. Production of excitons in grazing collisions of protons with LiF surfaces: An onion model

    Energy Technology Data Exchange (ETDEWEB)

    Miraglia, J. E.; Gravielle, M. S. [Instituto de Astronomia y Fisica del Espacio, Consejo Nacional de Investigaciones Cientificas y Tecnicas and Departamento de Fisica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Casilla de Correo 67, Sucursal 28, (C1428EGA) Buenos Aires (Argentina)

    2011-12-15

    In this work we evaluate the production of excitons of a lithium fluoride crystal induced by proton impact in the intermediate and high energy regime (from 100 keV to 1 MeV). A simple model is proposed to account for the influence of the Coulomb grid of the target by dressing crystal ions to transform them in what we call onions. The excited states of these onions can be interpreted as excitons. Within this model, total cross section and stopping power are calculated by using the first Born and the continuum distorted-wave (CDW) eikonal initial-state (EIS) approximations. We found that between 7 and 30 excitons per incident proton are produced in grazing collisions with LiF surfaces, becoming a relevant mechanism of inelastic transitions.

  9. 78 FR 52099 - Onions Other Than Bermuda-Granex-Grano/Creole; Bermuda-Granex-Grano

    Science.gov (United States)

    2013-08-22

    ... Washington State, which is outside of marketing order 958, has packed mixed colors of larger Walla Walla type... permitting mixed colors when designated as a specialty or mixed pack will facilitate the marketing of onions..., August 22, 2013 / Proposed Rules#0;#0; [[Page 52099

  10. Optimal control of indoor climate in agricultural storage facilities for potatoes and onions

    NARCIS (Netherlands)

    Lukasse, L.J.S.; Maldegem, van J.; Dierkes, E.; Voort, van der A.J.; Kramer-Cuppen, de J.E.; Kolk, van der G.

    2009-01-01

    This paper presents the use of receding horizon optimal control (RHOC) for optimal climate control in storage facilities for potatoes and onions. RHOC is used on a supervisory level above the classical feedback climate controller. Some theoretical issues on RHOC are discussed, amongst which a

  11. Haemato-protective influence of dietary fenugreek (Trigonella foenum-graecum L.) seeds is potentiated by onion (Allium cepa L.) in streptozotocin-induced diabetic rats.

    Science.gov (United States)

    Pradeep, Seetur R; Srinivasan, Krishnapura

    2018-02-01

    We have recently reported the beneficial modulation of metabolic abnormalities and oxidative stress in diabetic rats by dietary fenugreek seeds and onion. This investigation evaluated the protective influence of dietary fenugreek seeds (100 g kg -1 ) and onion (30 g kg -1 ) on erythrocytes of streptozotocin-induced diabetic rats, through modulation of reduced haematological indices and antisickling potency. This study also evaluated the altered erythrocyte membrane lipid profile and beneficial countering of increased lipid peroxidation, osmotic fragility, along with reduced membrane fluidity and deformability, nitric oxide production and echinocyte formation. Dietary fenugreek seeds and onion appeared to counter the deformity and fragility of erythrocytes partially in diabetic rats by their antioxidant potential and hypocholesterolemic property. The antisickling potency of these spices was accomplished by a substantial decrease in echinocyte population and AGEs in diabetic rats. Further insight into the factors that might have reduced the fluidity of erythrocytes in diabetic rats revealed changes in the cholesterol: phospholipid ratio, fatty acid profile, and activities of membrane-bound enzymes. Dietary fenugreek seeds and onion offered a beneficial protective effect to the red blood cells, the effect being higher with fenugreek + onion. This is the first report on the hemato-protective influence of a nutraceutical food component in diabetic situation. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  12. Desafios e oportunidades para o agronegócio da cebola no Brasil The challenges and the oportunities for the onion agribusiness

    Directory of Open Access Journals (Sweden)

    Nirlene J. Vilela

    2005-12-01

    Full Text Available Este trabalho teve por objetivo descrever alguns aspectos socioeconômicos relacionados à cebola no Brasil e detectar os principais desafios e oportunidades que o produto apresenta para o agronegócio. A cultura, de caráter tipicamente "familiar" (88%, gera cerca de 250 mil empregos somente no setor de produção. O consumo é estável em 85 mil t por mês. A consolidação do Mercosul e a conseqüente importação de cebola da Argentina somada à produção nacional tem causado excesso de oferta em alguns meses, gerando perdas e conseqüentes prejuízos para os produtores. O setor produtivo brasileiro necessita elevar o nível tecnológico para alcançar maior eficiência técnica e econômica. No entanto, é necessário que as regiões produtoras do Brasil e da Argentina tenham estabilidade de produção e que a quantidade ofertada seja suficiente para atender às necessidades de abastecimento em determinados períodos do ano, sem causar desequilíbio no mercado.The main objective of this work was to describe some socieconomic aspects of the onion production in Brazil and to detect the principal challenges and opportunities for the onion agribusiness. The onion cultivation in Brazil is a family activity (88%, generating about 250 thousand jobs directly involved in the production. The onion consumption in Brazil is nearly 85 thousand t per month. With the consolidation of the MERCOSUL, the onion import from Argentina and the national production has caused excess of supply in some months, generating losses and consequent damages for the producers. The Brazilian productive sector needs to improve the technological level of the onion production to reach greater technical and economic efficiency. However, it is necessary that Brazilian and Argentinian producers look for a better production forecast to avoid excess of supply during some periods of the year.

  13. Onions, myths, beliefs, fashion and reality in asthma.

    Science.gov (United States)

    Martínez-Gimeno, A

    2009-01-01

    Some myths and unsupported beliefs about asthma are very popular and enjoy general public acceptance and fairly strong support on the Internet. Onions for cough; dairy products avoidance for asthma; and some other popular myths are reviewed, along with some other medical and mixed (popular and medical) myths comparing their popular and scientific support. Classifying medical statements as realities or unsupported beliefs is a hard and serious work nowadays addressed by Evidence Based Medicine methods, which are not devoid of the influence of medical fashion: the medical community is more prone to accept fashionable statements compared to non-fashionable or old-fashioned statements.

  14. A prospective cohort study on the relationship between onion and leek consumption, garlic supplement use and the risk of colorectal carcinoma in the Netherlands

    NARCIS (Netherlands)

    Dorant, E.; Brandt, P.A. van den; Goldbohm, R.A.

    1996-01-01

    The association between onion and leek consumption, garlic supplement use and colon and rectum carcinoma among men and women was evaluated in the Netherlands Cohort Study, a large-scale prospective cohort study on diet and cancer. Onions, leeks, and garlic belong to the Allium genus and contain

  15. Fullerenes, nanotubes, onions and related carbon structures

    Energy Technology Data Exchange (ETDEWEB)

    Rao, C N.R.; Seshadri, Ram; Govindaraj, A; Sen, Rahul [Solid State and Structural Chemistry Unit, CSIR Centre of Excellence in Chemistry and Materials Research Centre, Indian Institute of Science, Bangalore (India)

    1995-12-01

    Fullerenes, containing five- and six-membered carbon rings, of which C{sub 6}0 and C{sub 7}0 are the prominent members, exhibit phase transitions associated with orientational ordering. When C{sub 6}0 is suitably doped with electrons, it shows novel superconducting and magnetic properties. We review these and other properties of fullerenes in bulk or in film form along with the preparative and structural aspects. Carbon nanotubes and onions (hyperfullerenes) are the other forms of carbon whose material properties have aroused considerable interest. Besides discussing these new forms of carbon, we briefly introduce other possible forms, such as those involving five-, six- and seven-membered rings and hybrids between diamond and graphite

  16. Microstructure characterization of onion (A.cepa) peels and thin films for dye sensitized solar cells

    Science.gov (United States)

    Abodunrin, T.; Boyo, A.; Usikalu, M.; Obafemi, L.; Oladapo, O.; Kotsedi, L.; Yenus, Z.; Maaza, M.

    2017-03-01

    A.cepa peels are obtained from mature onion bulbs. Because of the continuous need for energy, alternative avenues for producing energy are gaining importance. The motivation for this work is based on an urgent need to source energy from readily available waste materials like domestic onion peels. Dye sensitized solar cells (DSSCs) fabricated via doctor blade method and high temperature sintering from waste (onion peels) are investigated for their ability to convert solar to electrical energy. The charge carriers were revealed under phytochemical screening. Functional groups of compounds present in A.cepa peel were analyzed with Fourier transform in infrared (FTIR). The influence of different electrolyte sensitizer is observed on the DSSCs under standard air mass conditions of 1.5 AM. The microstructure properties of these A.cepa DSSCs were explored using scanning electron microscope with energy dispersive spectroscopy (SEM/EDS), x-ray diffraction and Fluorecence spectroscopy (XRF). The interfacial boundary between A.cepa dye, TiO2 framework of TiO2 and indium doped tin oxide (ITO) reveals several prominent anatase and rutile peaks. Photoelectric results, revealed dye-sensitized solar cells with a maximum power output of 126 W and incident photon to conversion energy (IPCE) of 0.13%.This work has established that A.cepa peels can be used as a source of micro-energy generation.

  17. Effects of plant density and cultivar on yield responses in onions (Allium cepa L. grown from seeds

    Directory of Open Access Journals (Sweden)

    Jan Rumpel

    2013-12-01

    Full Text Available Two field experiments were conducted to study the effect of plant density on yield, size grading and maturity of onion bulbs grown from seeds. In the first experiment carried out during 1991-1993, three onion cultivars (Hysam F1 , Mercato F1 and Sochaczewska were sown for intended densities of 20, 40, 60, 80,100 and 140 plants m-2, whereas in the second one, in 1996, six onion cultivars (Spirit F1, Summit F1, Hyduro F1, Armstrong F1, Renate F1 and Robusta were sown for intended densities of 40, 60 and 80 plants m-2. The onions were grown on beds, 1,35 m wide, in 4 rows per bed (27+27+27+54 cm. Marketable yield increased with plant density, and depending on year was highest at 80 or 100 plants m-2. The average marketable yield of the 1991-1993 experiment increased from 20.5 t·ha-1 at 20 plants m-2 to 32.8 t·ha-1 at 80 plants m-2, whereas that of the 1996 experiment increased from 48,9 t-ha-1 at 40 plants m-2 to 59.0 t·ha-1 at 80 plants m-2, respectively. Yield of large bulbs decreased with density and was highest at 20-40 plants m-2, oposite to the yield of small bulbs, which was highest at the highest density of 140 plants·m-2. The medium bulb yield increased with density, at the some way as compared the total marketable yield. No greater effect of cultivar on bulb size grades was found and the existing differences were proportional to the total marketable yield . Plant density hastened maturity of onions, and at density of 140 plants m-2 the leaf fall-over occurred 9-10 days earlier as compared at density of 20 plants m-2. The cultiwars used. can be placed in the following order of decreasing productivity: 1 . Mercato F1, 2. Hysam F1 and 3. Sochaczewska, - in the first expeiiment (1991-93 and 1. Annstrong F1, 2. Spirit F1, 3. Robusta, 4. Renate F1, 5. Hyduro F1 and 6. Summit F1 - in the second experiment (1996, respectively.

  18. Productive and qualitative evaluation of onion cultivars under agro-climatic conditions of faisalabad

    International Nuclear Information System (INIS)

    Mushtaq, S.; Amjad, M.; Ziaf, K.; Cheema, K.L.

    2013-01-01

    Onion varieties are very specific in their photoperiod and vernalization requirements and therefore vary for yield, yield related traits and bolting in a specific agro-climate. Therefore, performance of nineteen onion varieties for these traits was evaluated at Vegetable Research Institute, Faisalabad. The maximum bolting percentage was recorded in Desi Red (46.67%) that indicates less vernalization requirement of this variety while it was the minimum in Faisal Red and VRIO-6 (13.33%). The cultivar Phulkara produced larger size bulbs (73.22 mm diameter) as well as highest yield (21.90 t ha/sup -1/) and bulb to neck diameter ratio (6.75). Similarly, minimum weight loss during curing was observed in Desi Red (4.64%), Pusa Red (4.76%) and Phulkara (4.83%), indicating higher dry matter contents while maximum weight loss (6%) was recorded in VRIO-6. Overall results revealed that both Phulkara and Desi Red are excellent for processing while Dark Red for cooking purpose under agro-climatic conditions of Faisalabad. (author)

  19. Changes in flavonoids of sliced and fried yellow onions (allium cepa L. var. zittauer) during storage at different atmospheric, temperature and light conditions

    DEFF Research Database (Denmark)

    Islek, Merve; Nilufer-Erdil, Dilara; Knuthsen, Pia

    2015-01-01

    Flavonoid changes in sliced and fried onions which were packed and stored at different atmospheric conditions (air, nitrogen and vacuum), temperatures (ambient, +5 and -18C) and light (dark or light) were investigated. Flavonoids were extracted using accelerated solvent extraction and analyzed us......, or -18C, vacuum or nitrogen atmosphere, under dark, preserved flavonoids for 21 days, whereas for fried onions, 7 days of storage at +5C, vacuum atmosphere under dark resulted in highest flavonoid content. © 2014 Wiley Periodicals, Inc.......Flavonoid changes in sliced and fried onions which were packed and stored at different atmospheric conditions (air, nitrogen and vacuum), temperatures (ambient, +5 and -18C) and light (dark or light) were investigated. Flavonoids were extracted using accelerated solvent extraction and analyzed...... using ultra performance liquid chromatography coupled with photodiode array detector. Total flavonoid content, quercetin-3,4'-O-diglucoside and quercetin-4'-O-monoglucoside contents in sliced reference onion samples were found as 1,570±176, 926±105 and 564±64μg q.e./g d.w., respectively. Frying did...

  20. Aktivitas Penyembuhan Luka Sediaan Topikal Ekstrak Bawang Merah (Allium cepa terhadap Luka Sayat Kulit Mencit (Mus Musculus (THE ACTIVITY OF TOPICAL EXTRACT OF ONIONS (ALLIUM CEPA ON WOUND HEALING PROCESS IN MICE (MUS MUSCULUS

    Directory of Open Access Journals (Sweden)

    Visa Yunanda

    2017-01-01

    Full Text Available Wound healing is a physiological response of the body to restore continuity, structure and function ofthe injured tissue. Onion is one of the plants that are empirically used by the community to heal wounds.The purpose of this study was to observe the effect of topical extract of onions (Allium cepa on woundhealing process in mice (Mus musculus strain BalbC. This study used the posttest-only control groupdesign with completely randomized design (CRD. The samples were 12 male mice with weight ranged of22-32 g divided into four treatments; i.e.: Vaseline, topical extract of onion 5%, topical extract of onion30% and topical extract of onion 55% with 3 replications. Wound healing evaluated macroscopically toobserve hyperemia, wound contraction, granulation, crusting and pus production; and microscopically bycounting the number of fibroblasts on day 10. Hyperemia, granulation, crusting and pus production wereanalyzed descriptively. Wound contraction and the number of fibroblasts were analyzed using ANOVA(p<0.05 and followed with Duncan’s test (p<0,05. Descriptive observations obtained hyperemia lasteduntil day 3, granulation in wounds used topical extract of onion 30% and 55% were faster than the other,crusting lasted until day 6 and the production of pus was not found in any treatment. Statistical testresults showed that topical extract of onion 55% significantly affected wound contraction and topicalextract of onion 30% and 55% significantly affected maturation, which characterized by the decreasednumber of fibroblasts. The results of this study showed that topical extract of onion had significant effect(p<0.05 on wound healing and the effect was in line with the increased concentration of the onion extract.

  1. Genecology and seed zones for tapertip onion in the US Great Basin

    Science.gov (United States)

    R. C. Johnson; Barbara C. Hellier; Ken W. Vance-Borland

    2013-01-01

    The choice of germplasm is critical for sustainable restoration, yet seed transfer guidelines are lacking for all but a few herbaceous species. Seed transfer zones based on genetic variability and climate were developed using tapertip onion (Allium acuminatum Hook.) collected in the Great Basin and surrounding areas in the United States. Bulbs from 53 locations were...

  2. Integration of gamma radiation and plant powders to protect Peanut and Onion from infestation with the Almond Moth Ephestia Cautella (WALKER

    International Nuclear Information System (INIS)

    RIZK, S.A.; BOSHRA, S.A.; MIKHAIEL, A.A.

    2006-01-01

    The use of different plant powders of Ambrosia Maritiama, Origanum Vulgare, Glycyrrhiza Glabra, Matricaria Chamomilla and Nigella sativa for protection of peanuts (shelled or unshelled) and onion from infestation by the almond moth, Ephestia Cautella, showed that G. Glabra caused 100% larval mortality in case of shelled peanut at 0.25 g which leads to zero infestation while 100% larval mortality was occurred also when G.Glabra was used at concentration 1.0 gram in unshelled peanut. In onion, 100% mortality was occurred when powders of G.Glabra and M.Chamomilla were used at concentration 0.5 gram and A.Maritiama at concentration 1.0 gram. The use of different plant powders leads to reduction in percent pupation and percent emergence comparing with the control. Also, the number of eggs laid per female was decreased in females treated with plant powders compared to the control and the concentration 1 gram prevent egg hatching for all plant powders used in shelled or unshelled peanut and onion. The use of LC 3 0 of plant powders beside the doses 100 or 150 Gy of gamma radiation leads to increase in percent mortality and decrease in percent infestation as the dose increase. The use of G.Glabra beside 100 or 150 Gy caused 100% mortality and prevent peanut inside shells from infestation, but the plant powders of A.Maritiama, N.Sativa and M.Chamomilla leads to the same result but with the dose 150 Gy. The use of LC 3 0 of plant powders beside 150 Gy leads to decrease in number of eggs and prevent egg hatching in peanut and onion except when N.sativa and M.Chamomilla used with onion

  3. Survival and transfer of murine norovirus 1, a surrogate for human noroviruses, during the production process of deep-frozen onions and spinach.

    Science.gov (United States)

    Baert, Leen; Uyttendaele, Mieke; Vermeersch, Mattias; Van Coillie, Els; Debevere, Johan

    2008-08-01

    The reduction of murine norovirus 1 (MNV-1) on onions and spinach by washing was investigated as was the risk of contamination during the washing procedure. To decontaminate wash water, the industrial sanitizer peracetic acid (PAA) was added to the water, and the survival of MNV-1 was determined. In contrast to onions, spinach undergoes a heat treatment before freezing. Therefore, the resistance of MNV-1 to blanching of spinach was examined. MNV-1 genomic copies were detected with a real-time reverse transcription PCR assay in PAA-treated water and blanched spinach, and PFUs (representing infectious MNV-1 units) were determined with a plaque assay. A onion bulbs and spinach leaves. More than 3 log PFU of MNV-1 was transmitted to onion bulbs and spinach leaves when these vegetables were washed in water containing approximately 5 log PFU/ml. No decline of MNV-1 occurred in used industrial spinach wash water after 6 days at room temperature. A concentration of 20 ppm of PAA in demineralized water (pH 4.13) and in potable water (pH 7.70) resulted in reductions of 2.88 +/- 0.25 and 2.41 +/- 0.18 log PFU, respectively, after 5 min of exposure, but no decrease in number of genomic copies was observed. No reduction of MNV-1 PFUs was observed on frozen onions or spinach during storage for 6 months. Blanching spinach (80 degrees C for 1 min) resulted in at least 2.44-log reductions of infectious MNV-1, but many genomic copies were still present.

  4. Effect of low doses gamma irradiation on seed, bulblets and bulbs of onion

    International Nuclear Information System (INIS)

    Al-Oudat, Mohammad

    1991-10-01

    Presowing seed irradiation has been reported as a useful application of radiation in agriculture to stimulate growth and increase the yield of certain crops. To the best of our knowledge the feasibility of this treatment has not yet been tested on onion in Syria. The effect of low doses gamma irradiation on onion seeds, bulblets and bulbs of two local varieties, red and white, was studied during three consecutive seasons (1986 - 1988). Air dried seeds were irradiated by gamma rays from 137 Cs source. Five, 10, 15, 20 and 30 GY, were applied at dose rate of 9.8 Gy/min. The irradiation of onion bulblets and bulbs were carried out with gamma-rays from 60 Co source at a dose rate of 0.5 Gy/min. using 1, 2, 3, 4 and 5 Gy. Within 7 - 10 days after irradiation, both controlled and irradiated seeds, bulblets and bulbs were sown in the field in complete randomized block design with 4 replicates. Irradiation of seeds with doses of 5, 10 and 15 Gy led to highly significant increases in bulblets yield in the three seasons. The increases ranged from 14.5 to 22.1 for red variety and from 16.2 to 22.3 for white variety. The irradiation of bulblets with 1 and 2 Gy increase significantly the yield of bulbs by 21.6 - 26.0% for red variety and 21.6 - 24.4% for white variety. A considerable increase in seed yield was obtained after irradiation of bulbs with 1 and 2 Gy doses. The average increment was about 21.0% for both varieties. Large scale application were performed in 1989 and 1990 using doses of 10 Gy for seeds and 1 Gy for bulblets and bulbs. A considerable increase in the yield was obtained. The average percentage increment was 16.9% and 23.3% for seeds, 18.6 and 20.9% for bulblets, 24.8 and 27.3% for bulbs, for red and white varieties respectively. Therefore, presowing irradiation of seeds, bulblets and bulbs of onion with low doses of gamma-rays (5 - 15 Gy for seeds and 1 - 2 Gy for bulblets and bulbs) can be of practical application resulting in improvement of yield of

  5. Field assessment of the susceptibility of onion cultivars to thrips attack – preliminary results

    Directory of Open Access Journals (Sweden)

    Pobożniak Maria

    2016-03-01

    Full Text Available Field experiments on the susceptibility of onion to thrips infestation were carried out in 2012 and 2013. The investigation used 18 cultivars and 5 breeding lines of Allium cepa, and the species A. fistulosum and A. roylei. In 2012 A. fistulosum was one of the most infested plants, whereas in 2013 infestation was no more than average. The most infested cultivars of A. cepa were ‘Efekt’, ‘Alonso F1’, ‘Alibaba’ and ‘Wama’ and the ‘NOE5’ breeding line. In 2012 the extent of damage ranged from 12 to 55%, in 2013 from 20 to 30%. The ‘NOE2’, ‘NOE3’ and ‘Wama’ cultivars were the most seriously damaged by thrips. Leaf damage to A. fistulosum was minimal. No damage was caused to the wild species A. roylei by feeding onion thrips.

  6. Search for compounds contributing to onion-like off-flavor in beer and investigation of the cause of the flavor.

    Science.gov (United States)

    Noba, Shigekuni; Yako, Nana; Kobayashi, Minoru; Masuda, Susumu; Watanabe, Tetsuya

    2017-10-01

    Onion-like off-flavor is a highly undesirable property in beer. Although several compounds that impart onion-like odors have been identified, the individual contribution of these compounds to the onion-like off-flavor in beer is not clear. In the present study, we searched for compounds that impart an onion-like odor by gas chromatography (GC)-olfactometry. The analysis of several types of beer revealed that 2-mercapto-3-methyl-1-butanol (2M3MB) and 3-mercapto-3-methyl-1-butanol (3M3MB) were possible causative compounds. Based on the difference threshold values in beer (0.13 ng/mL for 2M3MB and 17.5 ng/mL for 3M3MB) and the quantification values of these compounds in beer samples, only 2M3MB was considered to contribute to the onion-like off-flavor in beer. A further formation factor analysis of 2M3MB revealed that 2M3MB was formed in hopped wort after fermentation, and that the concentration of 2M3MB increased following the hot aeration treatment of wort. These results suggest that preventing the hot aeration of wort is a key factor for reducing 2M3MB levels in beer. In a previous report, 3-methyl-2-buten-1-ol (3MBol) was speculated to be the precursor of 2M3MB and 3M3MB; however, the results of the present quantification analysis and wort addition tests indicate that 3MBol did not contribute to the formation of 2M3MB in the brewing process and that unknown precursors of 2M3MB originated in wort. Identifying the precursor of 2M3MB may facilitate elucidation of the mechanism of 2M3MB formation. Copyright © 2017 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  7. Weed control using ammonium nonanoate and cultivation in organic Vidalia sweet onion production

    Science.gov (United States)

    Ammonium nonanoate is registered for weed control in certified organic crop production and may be useful to control cool-season weeds in organic Vidalia® sweet onion. Cultivation with a tine weeder has been identified as a cost-effective means of weed control, but delays in cultivation cause some w...

  8. Sensory quality of irradiated onion and garlic bulbs

    International Nuclear Information System (INIS)

    Curzio, O.A.; Urioste, A.M.

    1994-01-01

    The radioinhibition process has shown to prolong shelf-life of ''Valenciana sintetica 14'' onion variety and ''Colorado'' garlic variety. Sensory attributes of the irradiated bulbs were tested monthly by trained judges during extended storage in warehouse conditions (6-32C, R.H. 40-50%). The sensory properties observed were external and internal appearance, firmness and odor. The irradiated bulbs were judged to be superior in quality with respect to internal and external appearance (p 0.01) and firmness (p 0.01), after 180 days postharvest. The irradiated bulbs showed no difference in odor (p 0.05), when compared to unirradiated ones, through the storage period

  9. Pantoea allii sp. nov., isolated from onion plants and seed.

    Science.gov (United States)

    Brady, Carrie L; Goszczynska, Teresa; Venter, Stephanus N; Cleenwerck, Ilse; De Vos, Paul; Gitaitis, Ronald D; Coutinho, Teresa A

    2011-04-01

    Eight yellow-pigmented, Gram-negative, rod-shaped, oxidase-negative, motile, facultatively anaerobic bacteria were isolated from onion seed in South Africa and from an onion plant exhibiting centre rot symptoms in the USA. The isolates were assigned to the genus Pantoea on the basis of phenotypic and biochemical tests. 16S rRNA gene sequence analysis and multilocus sequence analysis (MLSA), based on gyrB, rpoB, infB and atpD sequences, confirmed the allocation of the isolates to the genus Pantoea. MLSA further indicated that the isolates represented a novel species, which was phylogenetically most closely related to Pantoea ananatis and Pantoea stewartii. Amplified fragment length polymorphism analysis also placed the isolates into a cluster separate from P. ananatis and P. stewartii. Compared with type strains of species of the genus Pantoea that showed >97 % 16S rRNA gene sequence similarity with strain BD 390(T), the isolates exhibited 11-55 % whole-genome DNA-DNA relatedness, which confirmed the classification of the isolates in a novel species. The most useful phenotypic characteristics for the differentiation of the isolates from their closest phylogenetic neighbours are production of acid from amygdalin and utilization of adonitol and sorbitol. A novel species, Pantoea allii sp. nov., is proposed, with type strain BD 390(T) ( = LMG 24248(T)).

  10. Comparison of the urinary excretion of quercetin glycosides from red onion and aglycone from dietary supplements in healthy subjects: a randomized, single-blinded, cross-over study.

    Science.gov (United States)

    Shi, Yuanlu; Williamson, Gary

    2015-05-01

    Some intervention studies have shown that quercetin supplementation can regulate certain biomarkers, but it is not clear how the doses given relate to dietary quercetin (e.g. from onion). We conducted a two-period, two-sequence crossover study to compare the bioavailability of quercetin when administered in the form of a fresh red onion meal (naturally glycosylated quercetin) or dietary supplement (aglycone quercetin) under fasting conditions. Six healthy, non-smoking, adult males with BMI 22.7 ± 4.0 kg m(-2) and age 35.3 ± 12.3 y were grouped to take the two study meals in random order. In each of the 2 study periods, one serving of onion soup (made from 100 g fresh red onion, providing 156.3 ± 3.4 μmol (47 mg) quercetin) or a single dose of a quercetin dihydrate tablet (1800 ± 150 μmol (544 mg) of quercetin) were administered following 3 d washout. Urine samples were collected up to 24 h, and after enzyme deconjugation, quercetin was quantified by LC-MS. The 24 h urinary excretion of quercetin (1.69 ± 0.79 μmol) from red onion in soup was not significantly different to that (1.17 ± 0.44 μmol) for the quercetin supplement tablet (P = 0.065, paired t-test). This means that, in practice, 166 mg of quercetin supplement would be comparable to about 10 mg of quercetin aglycone equivalents from onion. These data allow intervention studies on quercetin giving either food or supplements to be more effectively compared.

  11. Gamma-irradiated onions as a biological indicator of radiation dose

    International Nuclear Information System (INIS)

    Vaijapurkar, S.G.; Agarwal, Deepshikha; Chaudhuri, S.K.; Ram Senwar, Kana; Bhatnagar, P.K.

    2001-01-01

    Post-irradiation identification and dose estimation are required to assess the radiation-induced effects on living things in any nuclear emergency. In this study, radiation-induced morphological/cytological changes i.e., number of root formation and its length, shooting length, reduction in mitotic index, micronuclei formation and chromosomal aberrations in the root tip cells of gamma-irradiated onions at lower doses (50-2000 cGy) are reported. The capabilities of this biological species to store the radiation-induced information are also studied

  12. Staining human lymphocytes and onion root cell nuclei with madder root.

    Science.gov (United States)

    Cücer, N; Guler, N; Demirtas, H; Imamoğlu, N

    2005-01-01

    We performed staining experiments on cells using natural dyes and different mordants using techniques that are used for wool and silk dyeing. The natural dye sources were madder root, daisy, corn cockle and yellow weed. Ferrous sulfate, copper sulfate, potassium tartrate, urea, potassium aluminum sulfate and potassium dichromate were used as mordants. Distilled water, distilled water plus ethanol, heptane, and distilled water plus methanol were used as solvents. All dye-mordant-solvent combinations were studied at pH 2.4, 3.2 and 4.2. The generic staining procedure was to boil 5-10 onion roots or stimulated human lymphocyte (SHL) preparations in a dye bath on a hot plate. Cells were examined at every half hour. For multicolor staining, madder-dyed lymphocytes were decolorized, then stained with Giemsa. The AgNOR technique was performed following the decolorization of Giemsa stained lymphocytes. Good results were obtained for both onion root cells and lymphocytes that were boiled for 3 h in a dye bath that included 4 g madder root, 4 g ferrous sulfate as mordant in 50 ml of 1:1 (v/v) methanol:distilled water. The pH was adjusted to 4.2 with 6 ml acetic acid. We conclude that madder root has potential as an alternative dye for staining biological materials.

  13. Bioinspired onion epithelium-like structure promotes the maturation of cardiomyocytes derived from human pluripotent stem cells.

    Science.gov (United States)

    Xu, Cong; Wang, Li; Yu, Yue; Yin, Fangchao; Zhang, Xiaoqing; Jiang, Lei; Qin, Jianhua

    2017-08-22

    Organized cardiomyocyte alignment is critical to maintain the mechanical properties of the heart. In this study, we present a new and simple strategy to fabricate a biomimetic microchip designed with an onion epithelium-like structure and investigate the guided behavior of human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) on the substrate. The hiPSC-CMs were observed to be confined by the three dimensional surficial features morphologically, analogous to the in vivo microenvironment, and exhibited an organized anisotropic alignment on the onion epithelium-like structure with good beating function. The calcium imaging of hiPSC-CMs demonstrated a more mature Ca 2+ spark pattern as well. Furthermore, the expression of sarcomere genes (TNNI3, MYH6 and MYH7), potassium channel genes (KCNE1 and KCNH2), and calcium channel genes (RYR2) was significantly up-regulated on the substrate with an onion epithelium-like structure instead of the surface without the structure, indicating a more matured status of cardiomyocytes induced by this structure. It appears that the biomimetic micropatterned structure, analogous to in vivo cellular organization, is an important factor that might promote the maturation of hiPSC-CMs, providing new biological insights to guide hiPSC-CM maturation by biophysical factors. The established approach may offer an effective in vitro model for investigating cardiomyocyte differentiation, maturation and tissue engineering applications.

  14. Aspects of gametogenesis and radiation pathology in the onion fly, Hylemya antiqua (Meigen) : 1. Gametogenesis

    NARCIS (Netherlands)

    Theunissen, J.A.B.M.

    1976-01-01

    In the scope of a genetic control research project gametogenesis of the onion fly, Hylemya antiqua (Meigen), is studied as a base for investigations on radiation histopathology of the gonads.

    Various cytological, histological, electronmicroscopical and

  15. Determination of the Effects of Nutrient sources on Enhancement of Crop Tolerance to Bean Root Rot and Bean Stem Maggot in Western Kenya

    International Nuclear Information System (INIS)

    Otsyula, R.M.; Nderitu, J.H.

    1999-01-01

    Field bean phaseolus vulgaris tolerance to root rot (BRR) and bean stem maggot (BSM) is enhanced by improvement of soil nutrients. Organic and inorganic sources of soil nutrients were evaluated in this study to determine their effects on crop tolerance to BRR and BSM. Three variety of GLP 585 susceptible to BRR and BSM; GLP X92 tolerant to BRR and BSM; and KK-8 resistant to BRR and BSM were used. The study was conducted in farmer's field with high level of BRR and BSM over three seasons in a split plot design. Nutrient sources were laid down in main plots while varieties were in subplots. KK-8 gave the highest plant survival and yield over the seasons. GLP 585 had the lowest mean yield and plant survival. Crop tolerance was greatly improved by application of DAP as applied as nutrient sources and varieties for crop tolerance were identified

  16. Matran (50% clove oil) broadcast application for broadleaf weed control in spring-transplanted onions

    Science.gov (United States)

    Although corn gluten meal has shown promise as an early-season pre-emergent organic herbicide in sweet onion production, uncontrolled weeds can inflict serious yield reductions by the end of the growing season. Research was conducted in southeast Oklahoma to determine the effectiveness of Matran EC...

  17. Effect of γ-irradiation on volatile compounds of dried Welsh onion (Allium fistulosum L.)

    International Nuclear Information System (INIS)

    Gyawali, Rajendra; Seo, Hye-Young; Lee, Hyun-Ju; Song, Hyun-Pa; Kim, Dong-Ho; Byun, Myung-Woo; Kim, Kyong-Su

    2006-01-01

    The volatile compounds of γ-irradiated dried Welsh onion were isolated by simultaneous distillation-extraction (SDE) technique and then analyzed by gas chromatography-mass spectrometry (GC-MS) along with their non-irradiated counterparts. A total of 35 volatile compounds were identified in non-irradiated and 1 kGy irradiated samples and 36 volatile compounds were identified in 3, 5, 10 and 20 kGy irradiated samples so far belong to chemical classes of acid, alcohol, aldehyde, ester, furan, ketone and S-containing compound. S-containing compounds were detected as major volatile compounds of all experimental samples. Though the content of several compounds was increased after irradiation, content of major S-containing compounds was found to decreased in the process. Application of high-dose irradiation if required for microbial decontamination of dried Welsh onion is feasible as it enhanced the total concentration of volatile compounds by 31.60% and 24.85% at 10 and 20 kGy, respectively

  18. Onion skin waste as a valorization resource for the by-products quercetin and biosugar.

    Science.gov (United States)

    Choi, In Seong; Cho, Eun Jin; Moon, Jae-Hak; Bae, Hyeun-Jong

    2015-12-01

    Onion skin waste (OSW), which is produced from processed onions, is a major industrial waste. We evaluated the use of OSW for biosugar and quercetin production. The carbohydrate content of OSW was analyzed, and the optimal conversion conditions were evaluated by varying enzyme mixtures and loading volumes for biosugar production and quercetin extraction. The enzymatic conversion rate of OSW to biosugar was 98.5% at 0.72 mg of cellulase, 0.16 mg of pectinase, and 1.0mg of xylanase per gram of dry OSW. Quercetin extraction also increased by 1.61-fold after complete enzymatic hydrolysis. In addition, the newly developed nano-matrix (terpyridine-immobilized silica-coated magnetic nanoparticles-zinc (TSMNP-Zn matrix) was utilized to separate quercetin from OSW extracts. The nano-matrix facilitated easy separation and purification of quercetin. Using the TSMNP-Zn matrix the quercetin was approximately 90% absorbed. In addition, the recovery yield of quercetin was approximately 75% after treatment with ethylenediaminetetraacetic acid. Copyright © 2015 Elsevier Ltd. All rights reserved.

  19. Incidence of bacterial diseases associated with irrigation methods on onions (Allium cepa).

    Science.gov (United States)

    Chorolque, A; Pozzo Ardizzi, C; Pellejero, G; Aschkar, G; García Navarro, F J; Jiménez Ballesta, R

    2018-04-24

    In the last decade, diseases of bacterial origin in onions have increased and this has led to significant losses in production. These diseases are currently observed in both the Old and New Worlds. The aim of the experimental work reported here was to evaluate whether the irrigation method influences the incidence of diseases of bacterial origin. In cases where the inoculum was natural, the initial incidence of Soft Bacterial Rot was not manifested in any treatment in the first year, whereas at the end of the conservation period all treatments had increased incidences of infection. Sprinkler irrigation (8%) was statistically differentiated from the other treatments, for which the final incidence was similar (4.5%). For all irrigation treatments, the final incidence of Bacterial Soft Rot decreased or remained stable towards the end of the cycle, with the exception of sprinkler irrigation in 2015, which increased. It can be inferred from the results that the irrigation method does have an influence on the incidence of diseases of bacterial origin in the post-harvest stage for onions. This article is protected by copyright. All rights reserved.

  20. Dosimetry for commissioning and quality control in the irradiation of onions

    International Nuclear Information System (INIS)

    Biramontri, S.; Thongmitr, W.; Wanitsuksombut, W.

    1989-01-01

    Onions are a highly marketed produce in Thailand, and because of the climate, shelf-life is fairly limited during storage in markets and households. An alternative to systemic chemical treatment (e.g. maleic hydrazide) for sprouting inhibition is the application of absorbed doses of ionizing radiation of about 100 Gy (Matsuyama and Umeda (1983) Preservation of Food by Ionizing Radiation, Vol. III, Chap. 6, p. 159, CRC Press, Boca Raton, Fla, U.S.A.). (author)

  1. Model system for plant cell biology: GFP imaging in living onion epidermal cells

    Science.gov (United States)

    Scott, A.; Wyatt, S.; Tsou, P. L.; Robertson, D.; Allen, N. S.

    1999-01-01

    The ability to visualize organelle localization and dynamics is very useful in studying cellular physiological events. Until recently, this has been accomplished using a variety of staining methods. However, staining can give inaccurate information due to nonspecific staining, diffusion of the stain or through toxic effects. The ability to target green fluorescent protein (GFP) to various organelles allows for specific labeling of organelles in vivo. The disadvantages of GFP thus far have been the time and money involved in developing stable transformants or maintaining cell cultures for transient expression. In this paper, we present a rapid transient expression system using onion epidermal peels. We have localized GFP to various cellular compartments (including the cell wall) to illustrate the utility of this method and to visualize dynamics of these compartments. The onion epidermis has large, living, transparent cells in a monolayer, making them ideal for visualizing GFP. This method is easy and inexpensive, and it allows for testing of new GFP fusion proteins in a living tissue to determine deleterious effects and the ability to express before stable transformants are attempted.

  2. Production of D-tagatose and bioethanol from onion waste by an intergrating bioprocess.

    Science.gov (United States)

    Kim, Ho Myeong; Song, Younho; Wi, Seung Gon; Bae, Hyeun-Jong

    2017-10-20

    The rapid increase of agricultural waste is becoming a burgeoning problem and considerable efforts are being made by numerous researchers to convert it into a high-value resource material. Onion waste is one of the biggest issues in a world of dwindling resource. In this study, the potential of onion juice residue (OJR) for producing valuable rare sugar or bioethanol was evaluated. Purified Paenibacillus polymyxaL-arabinose isomerase (PPAI) has a molecular weight of approximately 53kDa, and exhibits maximal activity at 30°C and pH 7.5 in the presence of 0.8mM Mn 2+ . PPAI can produce 0.99g D-tagatose from 10g OJR. In order to present another application for OJR, we produced 1.56g bioethanol from 10g OJR through a bioconversion and fermentation process. These results indicate that PPAI can be used for producing rare sugars in an industrial setting, and OJR can be converted to D-tagatose and bioethanol. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Quality evaluation of Argentina onion irradiated, transported and stored

    International Nuclear Information System (INIS)

    Walder, J.M.M.; Domarco, R.E.; Spoto, M.H.F.; Blumer, L.; Curzio, O.A.; Croci, C.A.

    1995-01-01

    Onion bulbs of the Valenciana Sintetica 14 variety from Argentina were irradiated with a dose of 60 Gy gamma rays at 30-40 days after harvest. Treated and control lots (40 bags of 20 Kg each) were send by road to Piracicaba, SP, Brazil, and stored up to 180 days in a warehouse at 20-28 0 C and relative humidity ranging from 50 to 100%. Monthly quality control was done by physical and sensorial analysis. Radiation treatment reduced the weight loss of the bulbs and increased the percentage of marketable bulbs without affecting its sensorial properties. (author). 4 refs, 8 tabs

  4. Identification of bacteria pathogenic to or associated with onion (Allium cepa) based on sequence differences in a portion of the conserved gyrase B gene.

    Science.gov (United States)

    Bonasera, Jean M; Asselin, Jo Ann E; Beer, Steven V

    2014-08-01

    We have developed a method for the identification of Gram-negative bacteria, particularly members of the Enterobacteriaceae, based on sequence variation in a portion of the gyrB gene. Thus, we identified, in most cases to species level, over 1000 isolates from onion bulbs and leaves and soil in which onions were grown. Copyright © 2014 Elsevier B.V. All rights reserved.

  5. INTERNATIONAL TRADE WITH ONIONS

    Directory of Open Access Journals (Sweden)

    Radu Lucian PÂNZARU

    2013-01-01

    Full Text Available The paper refers to the global trade situation with onion, 2008-2010. Besides presenting the existing situation of the five units continents (Africa, America, Asia, Europe and Oceania study shows worldwide level of imports and exports, as well as its contribution to the performing acts of international exchange. Quantitative aspects of trade are filled with values data, that allowed the preparation of trade exchanges for this product. The main importer (in quantitative terms is represented by Asia, followed by Europe and at greater distance by the America, while the value of imports was dominated by operations performed in Europe, Asia and America - weights of 39.7 , 38.1 and 18.6% respective (of the world total. Concerning the quantity, the major players on the market exports are represented by Asia and Europe. They have dominated the world market, achieving 45.6 and respective 30.8% of world exports. America ranks third with a share of 17.4%. In terms of value, world exports were dominated by Asia, Europe and America (33.5, 32.7 and respective 26.7%, with a downward trend in the share of Asia and increasing European weights and American (compared to the specific situation of the quantities exported

  6. Nanometric onion-like hollow spheres in laser synthesized boron nitride ultrafine powder

    Energy Technology Data Exchange (ETDEWEB)

    Boulanger, L; Willaime, F [CEA Centre d` Etudes de Saclay, 91 - Gif-sur-Yvette (France). Direction des Technologies Avancees; Cauchetier, M [CEA Centre d` Etudes de Saclay, 91 - Gif-sur-Yvette (France). Dept. de Recherche sur l` Etat Condense, les Atomes et les Molecules

    1994-12-31

    TEM observations of ultra-fine B Cl{sub 3}-N H{sub 3} powders elaborated by an innovative method, laser pyrolysis, are presented. The resulting microstructures in the as received state and after thermal treatments show small nanometric scale configurations close to the ones met with carbon, such as an hollowed configuration of onions but at a much smaller scale than with carbon. 3 figs., 3 refs.

  7. A comparison study on the anti-leech effects of onion (Allium cepa L and ginger (Zingiber officinale with levamisole and triclabendazole

    Directory of Open Access Journals (Sweden)

    Bahmani Mahmoud

    2013-01-01

    Full Text Available Introduction: Leech may indwell in mucosa of the pharynx, tonsil, esophagus, nose, nasopharyngeal and rarely in larynx of hosts, however, the effective drugs against this parasite is scarce. This study was aimed to evaluate and compare the anti-leech effect of methanolic extract of onion (Allium cepa L and ginger (Zingiber officinale with levamisole and triclabendazole. Materials and Methods: In this study, 60 leeches (Limnatis nilotica were collected from south of Ilam. The anti-leech effect of methanolic extract of onion and ginger in comparison with levamisole and triclabendazole drugs (positive controls were evaluated. Distilled water was used as negative control. Paralysis and death of leeches were recorded in 720 minutes. Results: Lethal effect of methanolic extract of ginger against Limnatis nilotica was equal to levamisole and more than triclabendazole and methanolic extract of onion. Conclusion: Ginger equal to levamisole has anti-leech activity and its methanolic extract might be used against Limnatis nilotica.

  8. Incorporation of thymidine into onion root meristematic cell nuclei in presence of hydroxyurea and its role in recovery of mitotic activity

    International Nuclear Information System (INIS)

    Habdas, H.

    1977-01-01

    Hydroxyurea treatment of onion roots induced mitotic block which was released by transfer of bulbs to water, and also to some extent by addition of cold or 3 H-thymidine to hydroxyurea solutions. In presence of hydroxyurea there was noted very intense incorporation of 3 H-thymidine into cell nuclei, giving labelling index of 40-70%. However, all the mitotic figures appearing in presence of hydroxyurea and 3 H-thymidine were unlabelled. On the other hand, labelled mitotic figures were obtained when roots incubated with 3 H-thymidine in presence of hydroxyurea had been transferred to water. Incorporation of 3 H-uridine was unaffected by hydroxyurea. The results show that hydroxyurea arrests onion root meristematic cells, either in the S phase and the G 2 phase. Enhanced incorporation of 3 H-thymidine in the presence of hydroxyurea, and release by added thymidine of the mitotic block indicate that hydroxyurea induces in onion root meristematic cells a particular shortage of thymidylate. (author)

  9. Production of inulinase by Xanthomonas campestris pv phaseoli using onion (Allium cepa) and garlic (Allium sativum) peels in solid state cultivation.

    Science.gov (United States)

    Ayyachamy, M; Khelawan, K; Pillay, D; Permaul, K; Singh, S

    2007-10-01

    To access inulinase production by Xanthomonas campestris pv phaseoli using the submerged and solid state cultivation (SSC) methods. Various carbon sources, inulin-rich solid substrates and pure synthetic inulin were tested for their efficiency in inulinase induction. The highest inulinase production (17.42 IU ml(-1)) in submerged cultures of X. campestris was observed with inulin as a carbon source with an initial pH, temperature and agitation of 7.0, 37 degrees C and 150 rev min(-1) respectively. Among the various substrates, garlic peels (117 IU gds(-1)) and onion peels (101 IU gds(-1)) were found to be the best for inulinase production. The inulinase production level of X. campestris was 6.7-fold higher in garlic and 5.8-fold in onion, under optimized SSC conditions compared with the submerged culture. This is the first report on inulinase production from garlic and onion peels by X. campestris using SSC. SSC is an efficient method for inulinase production by X. campestris for commercial applications.

  10. Functionalization of multilayer fullerenes (carbon nano-onions) using diazonium compounds and "click" chemistry.

    Science.gov (United States)

    Flavin, Kevin; Chaur, Manuel N; Echegoyen, Luis; Giordani, Silvia

    2010-02-19

    A novel versatile approach for the functionalization of multilayer fullerenes (carbon nano-onions) has been developed, which involves the facile introduction of a variety of simple functionalities onto their surface by treatment with in situ generated diazonium compounds. This approach is complemented by use of "click" chemistry which was used for the covalent introduction of more complex porphyrin molecules.

  11. Response to increasing levels of NK in the production of onion (Allium cepa L. var. “Roja Arequipeña”

    Directory of Open Access Journals (Sweden)

    Julio Estuardo Amaya Robles

    2013-03-01

    Full Text Available With the objective to determine the influence of increasing levels of nitrogen and potassium in the growth and yield of onion bulbs, an experiment was carried in the Experimental Institute for Agricultural Research and Technology located in Pampas de San Juan, Laredo, La Libertad (Peru. The experimental design was randomized complete block with 3x4 factorial arrangement with three replicates and twelve treatments resulting from combinations 60, 120, 180 and 240 kg N ha-1 , and 40, 80 and 160 kg K2O has -1 , using 80 kg as a single dose of P2O5.ha-1 . We used certified seed onion var. "Roja Arequipeña”. Linear responses were obtained for the weight of bulbs with yields of 1.010 and 1.006 g -1 average of 10 bulbs, with combinations of N120xK80 x K80 and N60, and quadratic response to N dose of 120 kg.ha-1 with which promoted the increased production of onion bulbs.

  12. Onion Park Research Natural Area: Botanical and ecological resources inventory, mapping and analysis with recommendations towards the development of a long-term monitoring and research program

    Science.gov (United States)

    Earle F. Layser

    1992-01-01

    Onion Park is a floristically rich naturally occurring mountain meadow and wetland complex which is surrounded by subalpine forest. The grass- and wetlands comprising the Park contribute biological diversity to an otherwise predominantly lodgepole pine-forested, subalpine setting. Onion Park is located at 7400' elevation in the Little Belt Mountains, five miles...

  13. A systematic review of maggot debridement therapy for chronically infected wounds and ulcers.

    Science.gov (United States)

    Sun, Xinjuan; Jiang, Kechun; Chen, Jingan; Wu, Liang; Lu, Hui; Wang, Aiping; Wang, Jianming

    2014-08-01

    This study aimed to systematically evaluate maggot debridement therapy (MDT) in the treatment of chronically infected wounds and ulcers. We performed a meta-analysis referring to the PRISMA statement (Preferred Reporting Items for Systematic Reviews and Meta-Analyses). We searched for published articles in the following databases: PubMed, Web of Science, Embase, Wanfang (Chinese), and the China National Knowledge Infrastructure (CNKI). The latest search was updated on March 14, 2014. For dichotomous outcomes, the effects of MDT were expressed as the relative risk (RR) and 95% confidence interval (CI). For continuous outcomes with different measurement scales, we calculated the standardized mean difference (SMD). The pooled effects were estimated using a fixed effect model or random effect model based on the heterogeneity test. Subgroup analyses were performed according to the types of wounds or ulcers. MDT had a significantly increased positive effect on wound healing compared with conventional therapies, with a pooled RR of 1.80 (95% CI 1.24-2.60). The subgroup analysis revealed that the combined RRs were 1.79 (95% CI 0.95-3.38) for patients with diabetic foot ulcers (DFU) and 1.70 (95% CI 1.28-2.27) for patients with other types of ulcers. The time to healing of the ulcers was significantly shorter among patients treated with MDT, with a pooled SMD of -0.95 (95% CI -1.24, -0.65). For patients with DFU, the SMD was -0.79 (95% CI -1.18, -0.41), and for patients with other types of ulcers, the SMD was -1.16 (95% CI -1.63, -0.69). MDT not only shortened the healing time but also improved the healing rate of chronic ulcers. Therefore, MDT may be a feasible alternative in the treatment of chronic ulcers. Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.

  14. EFFECT OF CONCENTRATION AND FREQUENCY CROCOBER PLUS AS ORGANIC LIQUID FERTILIZER GIVING ON THE ONION CROPS (Allium ascalonicum L.

    Directory of Open Access Journals (Sweden)

    Jamilah Munir Munir

    2016-10-01

    Full Text Available Research on the effect of liquid organic fertilizer (POC Crocober plus and frequency of application to the crop of onion (Allium ascalonicum L. had been conducted in Sub Kajai, District lemur, Solok regency for 3 months starting from May to July 2015. The goal was to get the POC concentration and Crocober plus frequency to increase growth and yield of onion. Experiments using a randomized block design in a factorial form that consists of 2 factors. Factor 1 was 5 degree of concentration POC was 0% (P0, 2.5% (P1, 5% (P3, 7.5% (P4 and 10% (P5 while Factor 2, was the frequency of POC consists of two levels ie ; The data obtained and analyzed variance. If the F count larger than F table 5% followed by a test of Duncan Multiple Range Test (DMRT. The experimental results indicated that application of 5% POC Crocober plus concentration given weekly was the exact interaction to improve the growth and yield of onion with the result reached 13.83 tons/ha.Doi: 10.22216/jit.2014.v8i2.340

  15. Onion thrips (Thrips tabaci (Thysanoptera: Thripidae)) in cabbage on Prince Edward Island: observations on planting date and variety choice.

    Science.gov (United States)

    Blatt, Suzanne; Ryan, Andrew; Adams, Shelley; Driscoll, Joanne

    2015-01-01

    Onion thrips (Thrips tabaci Lindeman (Thysanoptera: Thripidae)) can be a pest in organic onion production on Prince Edward Island. This study was to examine the effect of planting time and variety on infestation levels and damage by onion thrips on cabbage (Brassicae oleracea capitala (L.)). A field site was planted with 2 main and 8 lesser varieties of cabbage over 4 planting dates. Some varieties were short season and harvested on July 31 with longer season varieties harvested on September 2. Blue sticky traps were used to capture thrips migrating into the field site from July 22-September 2. Traps were counted weekly and cabbage heads within the field site were visually surveyed for thrips. At harvest, heads were weighed and measured, thrips damage was assessed then the head was dissected and thrips counted on the first four layers of the head. Thrips exhibited a preference for Lennox over Bronco throughout the season although thrips populations were not high enough to effect economic damage in 2014. Planting date influenced cabbage head weight and size with later plantings yielding the largest heads. Use of planting date and variety to avoid thrips populations is discussed.

  16. Efficacy of molecular markers jnurf13 and AcPms1 for prediction of genotypes at the nuclear Ms locus in North American open-pollinated populations of onion

    Science.gov (United States)

    Seed of hybrid onion (Allium cepa L.) is produced using cytoplasmic male sterility (CMS) and the for most widely used source of onion CMS, male sterility is conditioned by the interaction of male-sterile (S) cytoplasm and the homozygous recessive genotype at the nuclear male-fertility locus Ms. Due ...

  17. Comparative studies of bioactive organosulphur compounds and antioxidant activities in garlic (Allium sativum L.), elephant garlic (Allium ampeloprasum L.) and onion (Allium cepa L.).

    Science.gov (United States)

    Kim, Sunyoung; Kim, Dan-Bi; Jin, Wenjie; Park, Junghyuck; Yoon, Wonjin; Lee, Yunyeol; Kim, Soyoung; Lee, Sanghee; Kim, Sungsoo; Lee, Ok-Hwan; Shin, Dongbin; Yoo, Miyoung

    2018-05-01

    We evaluated organosulphur compounds in Allium vegetables, including garlic, elephant garlic and onion, using high-performance liquid chromatography. Among organosulphur compounds, elephant garlic had considerable γ-glutamyl peptides, and garlic had the highest alliin content. Onion had low level of organosulphur compounds than did elephant garlic and garlic. In addition, antioxidant capacities were evaluated by oxygen radical absorbance capacity (ORAC) values and 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay. The results showed that garlic had the highest antioxidant capacity, followed by elephant garlic and onion. Furthermore, a positive correlation was observed between antioxidant activities and organosulphur compounds (R > 0.77). Therefore, our results indicate that there was a close relationship between antioxidant capacity and organosulphur compounds in Allium vegetables.

  18. Role of temperature and free moisture in onion flower blight. [Botrytis squamosa; Botrytis cinerea; and Botrytis allii

    Energy Technology Data Exchange (ETDEWEB)

    Ramsey, G.R.; Lorbeer, J.W.

    1986-06-01

    The cardinal temperatures at which onion umbels were blighted (after inoculation when two-thirds of the florets were open) with Botrytis squamosa, B. cinerea, and B. allii (isolated from blighted onion florets) were near 9, 21, and 27 C for B. squamosa, near 12, 21, and 30 C for B. cinerea, and near 9, 24, and 30 C for B. allii. The cardinal temperatures for mycelial growth (potato-dextrose agar) of B. squamosa, B. cinerea, and B. allii were near 5, 22, and 30 C for each fungus. The cardinal temperatures for conidial germination (on purified water agar) were near 6, 15, and 30 C for B. squamosa; 3, 18, and 33 C for B. cinerea; and 6, 24, and 33 C for B. allii. When the duration of free moisture on umbels after inoculation with the three pathogens was increased from 0 to 96 hr. the percentages of unopened florets, open florets, and immature seed capsules blighted at 21 C were increased significantly. Free moisture durations of 12-24, 6-12, and 6-12 hr were necessary for blighting of unopen florets, open florets, and immature seed capsules, respectively, by each pathogen at 21 C. A positive correlation between the amount of July rainfall and the natural incidence of onion flower blight was observed in Orange County, New York, from 1976 to 1981. 10 references, 2 figures, 1 table.

  19. Differential Response to Heat Stress in Outer and Inner Onion Bulb Scales.

    Science.gov (United States)

    Galsurker, Ortal; Doron-Faigenboim, Adi; Teper-Bamnolker, Paula; Daus, Avinoam; Lers, Amnon; Eshel, Dani

    2018-05-18

    Brown protective skin formation in onion bulbs can be induced by rapid postharvest heat treatment. Onions that were peeled to different depths and were exposed to heat stress showed that only the outer scale formed dry brown skin, whereas the inner scales maintained high water content and did not change color. Our results reveal that browning of the outer scale during heat treatment is due to an enzymatic process that is associated with high levels of oxidation components, such as peroxidase and quercetin glucoside. De-novo transcriptome analysis revealed differential molecular responses of the outer and inner scales to the heat stress. Genes involved in lipid metabolism, oxidation pathways and cell-wall modification were highly expressed in the outer scale during heating. Defense-response-related genes such as those encoding heat-shock proteins, antioxidative stress defense or production of osmoprotectant metabolites were mostly induced in the inner scale in response to the heat exposure. These transcriptomic data led to a conceptual model that suggests sequential processes for browning development and desiccation of the outer scales versus processes associated with defense response and heat tolerance in the inner scale. Thus, the observed physiological differences between the outer and inner scales is supported by the identified molecular differences.

  20. Natural antimicrobial peptide complexes in the fighting of antibiotic resistant biofilms: Calliphora vicina medicinal maggots.

    Directory of Open Access Journals (Sweden)

    Natalia Gordya

    Full Text Available Biofilms, sedimented microbial communities embedded in a biopolymer matrix cause vast majority of human bacterial infections and many severe complications such as chronic inflammatory diseases and cancer. Biofilms' resistance to the host immunity and antibiotics makes this kind of infection particularly intractable. Antimicrobial peptides (AMPs are a ubiquitous facet of innate immunity in animals. However, AMPs activity was studied mainly on planktonic bacteria and little is known about their effects on biofilms. We studied structure and anti-biofilm activity of AMP complex produced by the maggots of blowfly Calliphora vicina living in environments extremely contaminated by biofilm-forming germs. The complex exhibits strong cell killing and matrix destroying activity against human pathogenic antibiotic resistant Escherichia coli, Staphylococcus aureus and Acinetobacter baumannii biofilms as well as non-toxicity to human immune cells. The complex was found to contain AMPs from defensin, cecropin, diptericin and proline-rich peptide families simultaneously expressed in response to bacterial infection and encoded by hundreds mRNA isoforms. All the families combine cell killing and matrix destruction mechanisms, but the ratio of these effects and antibacterial activity spectrum are specific to each family. These molecules dramatically extend the list of known anti-biofilm AMPs. However, pharmacological development of the complex as a whole can provide significant advantages compared with a conventional one-component approach. In particular, a similar level of activity against biofilm and planktonic bacteria (MBEC/MIC ratio provides the complex advantage over conventional antibiotics. Available methods of the complex in situ and in vitro biosynthesis make this idea practicable.

  1. Natural antimicrobial peptide complexes in the fighting of antibiotic resistant biofilms: Calliphora vicina medicinal maggots.

    Science.gov (United States)

    Gordya, Natalia; Yakovlev, Andrey; Kruglikova, Anastasia; Tulin, Dmitry; Potolitsina, Evdokia; Suborova, Tatyana; Bordo, Domenico; Rosano, Camillo; Chernysh, Sergey

    2017-01-01

    Biofilms, sedimented microbial communities embedded in a biopolymer matrix cause vast majority of human bacterial infections and many severe complications such as chronic inflammatory diseases and cancer. Biofilms' resistance to the host immunity and antibiotics makes this kind of infection particularly intractable. Antimicrobial peptides (AMPs) are a ubiquitous facet of innate immunity in animals. However, AMPs activity was studied mainly on planktonic bacteria and little is known about their effects on biofilms. We studied structure and anti-biofilm activity of AMP complex produced by the maggots of blowfly Calliphora vicina living in environments extremely contaminated by biofilm-forming germs. The complex exhibits strong cell killing and matrix destroying activity against human pathogenic antibiotic resistant Escherichia coli, Staphylococcus aureus and Acinetobacter baumannii biofilms as well as non-toxicity to human immune cells. The complex was found to contain AMPs from defensin, cecropin, diptericin and proline-rich peptide families simultaneously expressed in response to bacterial infection and encoded by hundreds mRNA isoforms. All the families combine cell killing and matrix destruction mechanisms, but the ratio of these effects and antibacterial activity spectrum are specific to each family. These molecules dramatically extend the list of known anti-biofilm AMPs. However, pharmacological development of the complex as a whole can provide significant advantages compared with a conventional one-component approach. In particular, a similar level of activity against biofilm and planktonic bacteria (MBEC/MIC ratio) provides the complex advantage over conventional antibiotics. Available methods of the complex in situ and in vitro biosynthesis make this idea practicable.

  2. Flight movement and spatial distribution of immunomarked thrips in onion, potato, and tomato

    Directory of Open Access Journals (Sweden)

    Flávio Lemes Fernandes

    2015-05-01

    Full Text Available The objective of this work was to evaluate the spatial distribution of thrips in different crops, and the correlation between meterological parameters and the flight movements of this pest, using immunomarking. The experiment was conducted in cultivated areas, with tomato (Solanum lycopersicum, potato (Solanum tuberosum, and onion (Allium cepa; and non-cultivated areas, with weedy plants. The areas with tomato (100 days, potato (20 days, and weeds were sprayed with casein, albumin, and soy milk, respectively, to mark adult thrips; however, the areas with onion (50 days and tomato (10 days were not sprayed. Thrips were captured with georeferenced blue sticky traps, transferred into tubes, and identified by treatment area with the Elisa test. The dependence between the samples and the capture distance was determined using geostatistics. Meteorlogical parameters were correlated with thrips density in each area. The three protein types used for immunomarking were detected in different proportions in the thrips. There was a correlation between casein-marked thrips and wind speed. The thrips flew a maximum distance of 3.5 km and dispersed from the older (tomato to the younger crops (potato. The immunomarking method is efficient to mark large quantities of thrips.

  3. Onion seedling production in styrofoam trays under controlled environment, as summer-planted onions Plantas de cebola produzidas em bandejas sob cultivo protegido, no verão

    Directory of Open Access Journals (Sweden)

    Maria Cristina Veiga De Vincenzo

    2003-02-01

    Full Text Available Summer-planted onion (Allium cepa L. can increase grower profits, but the production of seedlings for transplant is difficult. This experiment was carried out in Piracicaba, SP, Brazil, with the objective of studying the behavior of the short-day onion hybrid Mercedes, in its initial stage, under controlled greenhouse environment, as summer-planted onions. Four formulated substrates, two different numbers of plants per cell were tested, and 288-cell styrofoam trays used. Trials were set up in split-plot design (n=3. Substrate and number of plants per cell represented main plots, and N application (2 and 3 times a week the subplots. The number of three plants per cell decrease the earlier onset of bulbing and increase plant height; substrates did not show differences; and the N application 3 times a week increased plant height and leaf dry weight, but did not result in earlier onset of bulbing.Uma das dificuldades do cultivo do verão da cebola (Allium cepa L., que permite maior lucro pois esta é a época de entressafra, é a produção de plantas para o transplante. Com o objetivo de verificar o comportamento na fase de muda do híbrido Mercedes, sob cultivo protegido como cultura de verão, o experimento foi instalado em estufa, em Piracicaba, SP com bandejas de isopor de 288 células. Foram testados quatro substratos. e dois números de plantas por células (3 e 4 plantas/célula, sendo sub-plot a suplementação mineral com nitrogênio 2 e 3 vezes por semana com nitrato de cálcio + nitrato de potássio. O delineamento experimental foi inteiramente casualizado, com fatorial 4 x 2 em parcelas subdivididas, e três repetições. A densidade de três plantas por célula apresentou menor taxa de bulbificação precoce e maior altura da planta; não houve diferença quanto aos substratos e que a aplicação de nitrogênio três vezes por semana apresentou os maiores valores de altura da planta e peso da matéria seca da parte aérea, n

  4. Agrobacterium tumefaciens mediated transformation of Allium cepa L.: the production of transgenic onions and shallots

    NARCIS (Netherlands)

    Zheng, S.J.; Khrustaleva, L.I.; Henken, G.; Sofiari, E.; Jacobsen, E.; Kik, C.; Krens, F.A.

    2001-01-01

    This paper describes the development of a reliable transformation protocol for onion and shallot (Allium cepa L.) which can be used year-round. It is based on Agrobacterium tumefaciens as a vector, with three-week old callus, induced from mature zygotic embryos, as target tissue. For the development

  5. Characterization of onion lectin (Allium cepa agglutinin) as an immunomodulatory protein inducing Th1-type immune response in vitro.

    Science.gov (United States)

    Prasanna, Vaddi K; Venkatesh, Yeldur P

    2015-06-01

    Onion (Allium cepa), a bulb crop of economic importance, is known to have many health benefits. The major objective of the present study is to address the immunomodulatory properties of onion lectin (A. cepa agglutinin; ACA). ACA was purified from onion extract by D-mannose-agarose chromatography (yield: ~1 mg/kg). ACA is non-glycosylated and showed a molecular mass of ~12 kDa under reducing/non-reducing SDS-PAGE; glutaraldehyde cross-linking indicated that ACA is a non-covalent tetramer of ~12 kDa subunits. Its N-terminal sequence (RNVLLNNEGL; UniProt KB Accn. C0HJM8) showed 70-90% homology to mannose-specific Allium agglutinins. ACA showed specific hemagglutination activity of 8200 units/mg and is stable in the pH range 6-10 and up to 45° C. The immunomodulatory activity of ACA was assessed using the macrophage cell line, RAW264.7 and rat peritoneal macrophages; at 0.1 μg/well, it showed a significant increase (6-8-fold vs. control) in the production of nitric oxide at 24h, and significantly stimulated (2-4-fold vs. control) the production of pro-inflammatory cytokines (TNF-α and IL-12) at 24h. ACA (0.1 μg/well) enhanced the proliferation of murine thymocytes by ~4 fold (vs. control) at 24h; however, ACA does not proliferate B cell-enriched rat splenocytes. Further, it significantly elevated the expression levels of cytokines (IFN-γ and IL-2) over the control in murine thymocytes. Taken together, purified ACA induces a Th1-type immune response in vitro. Though present in low amounts, ACA may contribute to the immune-boosting potential of the popular spice onion since considerable amounts are consumed on a daily basis universally. Copyright © 2015 Elsevier B.V. All rights reserved.

  6. Determination of the geographic origin of onions between three main production areas in Japan and other countries by mineral composition.

    Science.gov (United States)

    Ariyama, Kaoru; Aoyama, Yoshinori; Mochizuki, Akashi; Homura, Yuji; Kadokura, Masashi; Yasui, Akemi

    2007-01-24

    Onions (Allium cepa L.) are produced in many countries and are one of the most popular vegetables in the world, thus leading to an enormous amount of international trade. It is currently important that a scientific technique be developed for determining geographic origin as a means to detect fraudulent labeling. We have therefore developed a technique based on mineral analysis and linear discriminant analysis (LDA). The onion samples used in this study were from Hokkaido, Hyogo, and Saga, which are the primary onion-growing areas in Japan, and those from countries that export onions to Japan (China, the United States, New Zealand, Thailand, Australia, and Chile). Of 309 samples, 108 were from Hokkaido, 52 were from Saga, 77 were from Hyogo, and 72 were from abroad. Fourteen elements (Na, Mg, P, Mn, Co, Ni, Cu, Zn, Rb, Sr, Mo, Cd, Cs, and Ba) in the samples were determined by frame atomic adsorption spectrometry, inductively coupled plasma optical emission spectrometry, and inductively coupled plasma mass spectrometry. The models established by LDA were used to discriminate the geographic origin between Hokkaido and abroad, Hyogo and abroad, and Saga and abroad. Ten-fold cross-validations were conducted using these models. The discrimination accuracies obtained by cross-validation between Hokkaido and abroad were 100 and 86%, respectively. Those between Hyogo and abroad were 100 and 90%, respectively. Those between Saga and abroad were 98 and 90%, respectively. In addition, it was demonstrated that the fingerprint of an element pattern from a specific production area, which a crop receives, did not easily change by the variations of fertilization, crop year, variety, soil type, and production year if appropriate elements were chosen.

  7. Analysis of Factors Affecting the Success of Onions Development Program in Kampar Regency

    Science.gov (United States)

    Amalia; Putri, Asgami

    2017-12-01

    The purpose of this study is to analyze the factors influencing the success of the onion plant development program in Kampar regency. The research method used was the applied survey method using interview technique and observation or direct supervision on the location of the object. The briefing of the interviews as well as the accuracy of collecting the required data was guided by the structured questionnaires. Determination technique of location / region sampling was done purposively based on the potency and capacity of commodity development. While the respondents were taken by cluster purvosive sampling method in order to classify the samples in accordance with the purpose of the study, determined by as many as 100 people taken from members of the farmer group. Analytical technique used is by using Logic Regression Analysis to determine the factors that influence the success of the program seen from the characteristics of farmers. From the results of this study it can be concluded that the factors influencing the success of onion development program in Kampar regency were a age (X1), education (X2), income (X3), ethnicity (X4), jobs (X5) And family responsibility (X6) could be made as follows: Log Y (P/1-p) = -1.778 +X10.021 + X20.028 - X30.213 + X41.986 + X52.930 - X60.455 From the above equation, it can be explained that the attributes that are positively related are X1 (age), X2 (education), X4 (ethnicity) and X5 (jobs) while the negative correlates are X3 (income) and X6 (family responsibility). From the logical regression result it can be seen that the significant value influenced the dependent variable, so that when viewed from the table in the equation it was found that factors affecting the success rate of red onion development program in Kampar regency were X2 (education), X4 (ethnicity), X5 (jobs), and X6 (family responsibility).

  8. An effective method of increasing production rate of onion-like fullerenes

    Energy Technology Data Exchange (ETDEWEB)

    Liu Wen; Meng Qingsen [College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024 (China)], E-mail: lw915136@sina.com.cn

    2009-09-01

    Onion-like fullerenes (OLFs) were synthesized by arc discharge in benzene using graphite as electrode and ferrocene as catalyst. The effect of ferrocene on the morphologies and structures of the OLFs was investigated by HRTEM and XRD. Results show that ferrocene directly influenced the morphology and yield of OLFs: The degree of graphitization is better. The diameters of the OLFs can be controlled in the range between 10 and 30 nm The method described here suggests a novel and promising route to synthesize OLFs in large scales.

  9. Improving N-use efficiency in onion plants grown in desert soils using 15N through fertigation technique

    International Nuclear Information System (INIS)

    Thabet, E.M.A.; Abdallah, A.A.G.; Battah, N.S.

    2005-01-01

    Two field experiments were performed at the Experimental Farm of Nuclear Research Center, Atomic Energy Authority, Inshas, Egypt, during the two successive growing seasons of 2003 and 2004, in a clay loam textured soil. Seedlings of onion (Allium cepa L.) cultivar Giza-20 were transplanted in the field prepared with drip irrigation system for studying onion production under fertigation technique. Three nitrogen rates (50, 100 and 150 kg N/fed) as ammonium sulphate contain 21.2% N were used. These experiments aim to apply nitrogen along an extended time in order to maximize its use efficiency. Each rate was subjected to four splitting, i.e. one, two, three and four times of application, at 45 days from transplanting. Nitrogen was added every 15 days started 45 days after transplanting through a fertigation technique. In the first experiment (2003), labelled nitrogen (N-15) as ammonium sulphate contain 3.33% N-15 atom excess was applied for to estimate nitrogen use efficiency. One vegetative sample was harvested at 105 days from transplanting (15 days after the fourth application of nitrogen) to determine dry matter characters and N-15 analysis. At harvest, yield and yield components were determined for unit area. Results indicated that onion plants did not use more than about 20% of the applied nitrogen. Also yield did not respond to increasing rate up to splitting of nitrogen. The second growing season (2004) was conducted to measure bulbs yield under the same previous treatments

  10. Dyeing of γ-irradiated cotton with natural flavonoid dye extracted from irradiated onion shells (Allium cepa) powder

    International Nuclear Information System (INIS)

    Rehman, Fazal-ur; Adeel, Shahid; Shahid, Muhammad; Bhatti, Ijaz Ahmad; Nasir, Faiza; Akhtar, Nasim; Ahmad, Zulfiqar

    2013-01-01

    Powder of Onion shells as a source of natural flavonoid dye (Quercetin) and cotton fabrics were exposed to absorbed doses of 2, 4, 6, 8 and 10 kGy using Cs-137 gamma irradiator. Irradiated and un-irradiated dye powder was used for extraction of quercetin as well as antibacterial, hemolytic and antioxidant activities were also determined to observe the effect of radiation. Furthermore, color strength and colourfastness of irradiated fabrics were improved by using pre and post-mordants such as alum and iron. It is found that 4 kGy is the optimal absorbed dose for extraction of natural quercetin extracted from onion shells while maximum color strength and acceptable fastness properties are obtained on dyeing of irradiated fabric at 60 °C keeping M:L of 1:30 using 10% alum as pre-mordant and 6% alum as post-mordant. Gamma irradiation has not only improved the color strength of the dye using irradiated cotton but also that of colourfastness properties. - Highlights: • The optimal absorbed dose obtained for surface modification of cotton (RC) is 4 kGy. • The optimal absorbed dose for extraction of colorant from onion shell powder (RP) is 4 kGy. • Optimum dying conditions: 60 °C, 1 30 as M:L. • Optimum pre-mordant 10% alum and 6% alum as post-mordant. • At these optimum conditions: color strength and fastness properties are improved

  11. Capture of Nontarget Flies (Diptera: Lauxaniidae, Chloropidae, and Anthomyiidae on Traps Baited with Volatile Chemicals in Field-Crop Habitats

    Directory of Open Access Journals (Sweden)

    Louis S. Hesler

    2016-01-01

    Full Text Available Volatile chemicals increased trap catch of flies from the families Lauxaniidae [Homoneura bispina (Loew and Camptoprosopella borealis Shewell], Chloropidae (Olcella sp., and Anthomyiidae (Delia spp. in field crops. With lauxaniids, baiting with 2-phenylethanol on cotton-roll dispensers increased catch of H. bispina in two corn plot tests, and methyl salicylate increased trap catch in one test. Traps baited with methyl salicylate increased the catch of C. borealis. When using plastic-sachet dispensers, traps baited with methyl salicylate caught more H. bispina than ones baited with 2-phenylethanol, whereas traps baited with 2-phenylethanol caught more C. borealis than those with methyl salicylate. For chloropids, traps baited with 2-isopropyl-3-methoxypyrazine greatly increased catch of Olcella flies in corn and soybean. With anthomyiids, catch of male Delia flies in wheat increased with 2-phenylethanol on cotton rolls and with either 2-phenylethanol or methyl salicylate using plastic dispensers. In soybean, 2-phenylethanol formulated on cotton rolls or in plastic dispensers increased catch of male Delia flies, but methyl salicylate did not affect trap catch. Trap catch of female Delia flies did not vary among chemicals. In another test in soybean, trap catch of both male and female Delia flies was greater with 2-phenylethanol than with other volatile chemicals.

  12. Effect of low dose gamma irradiation on onion yield: Large scale application

    International Nuclear Information System (INIS)

    Al-Oudat, M.

    1993-01-01

    Large scale application of presowing gamma-irradiation of seeds, bulblets and bulbs of onion, performed in 1989, using the doses of 10 Gy for seeds and 1 Gy for bulblets and bulbs. The doses were chosen on the basis of previous experiments. Reliable increases in yield of seeds (19.3%), bulblets (18.9) and bulbs (31.4%) for red variety. and of 22.3% and 23.4% for seeds and bulbs of white variety were obtained. (author). 2 tabs

  13. Shielding evaluation of moving bed onion irradiator by radiometry

    International Nuclear Information System (INIS)

    Venkataramani, R.; Sangurdekar, P.R.; Sarangapani, R.; Raipurkar, D.R.; Mehta, S.K.; Shastri, S.P.; Patil, K.B.; Bongirwar, D.R.

    1994-01-01

    A moving bed onion irradiator made from m.s. cladded lead slab shields designed to hold 20 kCi of 60 Co source was evaluated by radiometry with an 8 Ci 60 Co source from CRC-2 radiography camera. Some shielding losses in the irradiator noted by radiometry could be visualized by a thermocole model of the complex shielding assembly. These were rectified by appropriate lead filling. Significant shielding losses noted at cladding layer positions of slabs were attributed to lack of interlocking features in the slabs. These had to be rectified by provision of 3 TVL of additional all round shielding supplemented by local shielding at some positions. (author). 1 fig., 1 tab

  14. Chromate adsorption mechanism on nanodiamond-derived onion-like carbon

    Energy Technology Data Exchange (ETDEWEB)

    Ko, Young-Jin [Center for Electronic Materials, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea (Korea, Republic of); Choi, Keunsu [Computational Science Research Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea (Korea, Republic of); Lee, Soonjae [Department of Earth and Environmental Sciences, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea (Korea, Republic of); Cho, Jung-Min [Center for Electronic Materials, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea (Korea, Republic of); Department of Materials Science and Engineering, Yonsei University, 262 Seongsanno, Seodaemun-Gu, Seoul 120-749, Republic of Korea (Korea, Republic of); Choi, Heon-Jin [Department of Materials Science and Engineering, Yonsei University, 262 Seongsanno, Seodaemun-Gu, Seoul 120-749, Republic of Korea (Korea, Republic of); Hong, Seok Won [Center for Water Resource Cycle Research, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea (Korea, Republic of); Choi, Jae-Woo, E-mail: plead36@kist.re.kr [Center for Water Resource Cycle Research, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea (Korea, Republic of); Department of Energy and Environmental Engineering, University of Science and Technology (UST), Daejeon 305-350, Republic of Korea (Korea, Republic of); Mizuseki, Hiroshi, E-mail: mizuseki@kist.re.kr [Computational Science Research Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea (Korea, Republic of); Lee, Wook-Seong, E-mail: wsleemk@gmail.com [Center for Electronic Materials, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea (Korea, Republic of)

    2016-12-15

    The onion-like carbon (OLC) was prepared as adsorbent and tested for the removal of chromate ions from aqueous solutions. The OLC was thermally derived from nanodiamond by vacuum annealing at 1000-2000 °C. An investigation was conducted the chromate adsorption mechanism of OLC, by analysing the temperature-dependent evolution of the various oxygen-carbon bonds and the chemisorbed water by X-ray photo electron spectroscopy, as well as by the first principle calculation of the bond energies for relevant bond configurations. The present work demonstrated the importance of the carbon-oxygen bond type and carbon dangling bonds for chromate adsorption, as well as for other anionic heavy metals adsorbed from wastewater and sewage.

  15. De Novo Assembly and Transcriptome Analysis of Bulb Onion (Allium cepa L.) during Cold Acclimation Using Contrasting Genotypes.

    Science.gov (United States)

    Han, Jeongsukhyeon; Thamilarasan, Senthil Kumar; Natarajan, Sathishkumar; Park, Jong-In; Chung, Mi-Young; Nou, Ill-Sup

    2016-01-01

    Bulb onion (Allium cepa) is the second most widely cultivated and consumed vegetable crop in the world. During winter, cold injury can limit the production of bulb onion. Genomic resources available for bulb onion are still very limited. To date, no studies on heritably durable cold and freezing tolerance have been carried out in bulb onion genotypes. We applied high-throughput sequencing technology to cold (2°C), freezing (-5 and -15°C), and control (25°C)-treated samples of cold tolerant (CT) and cold susceptible (CS) genotypes of A. cepa lines. A total of 452 million paired-end reads were de novo assembled into 54,047 genes with an average length of 1,331 bp. Based on similarity searches, these genes were aligned with entries in the public non-redundant (nr) database, as well as KEGG and COG database. Differentially expressed genes (DEGs) were identified using log10 values with the FPKM method. Among 5,167DEGs, 491 genes were differentially expressed at freezing temperature compared to the control temperature in both CT and CS libraries. The DEG results were validated with qRT-PCR. We performed GO and KEGG pathway enrichment analyses of all DEGs and iPath interactive analysis found 31 pathways including those related to metabolism of carbohydrate, nucleotide, energy, cofactors and vitamins, other amino acids and xenobiotics biodegradation. Furthermore, a large number of molecular markers were identified from the assembled genes, including simple sequence repeats (SSRs) 4,437 and SNP substitutions of transition and transversion types of CT and CS. Our study is the first to provide a transcriptome sequence resource for Allium spp. with regard to cold and freezing stress. We identified a large set of genes and determined their DEG profiles under cold and freezing conditions using two different genotypes. These data represent a valuable resource for genetic and genomic studies of Allium spp.

  16. De Novo Assembly and Transcriptome Analysis of Bulb Onion (Allium cepa L. during Cold Acclimation Using Contrasting Genotypes.

    Directory of Open Access Journals (Sweden)

    Jeongsukhyeon Han

    Full Text Available Bulb onion (Allium cepa is the second most widely cultivated and consumed vegetable crop in the world. During winter, cold injury can limit the production of bulb onion. Genomic resources available for bulb onion are still very limited. To date, no studies on heritably durable cold and freezing tolerance have been carried out in bulb onion genotypes. We applied high-throughput sequencing technology to cold (2°C, freezing (-5 and -15°C, and control (25°C-treated samples of cold tolerant (CT and cold susceptible (CS genotypes of A. cepa lines. A total of 452 million paired-end reads were de novo assembled into 54,047 genes with an average length of 1,331 bp. Based on similarity searches, these genes were aligned with entries in the public non-redundant (nr database, as well as KEGG and COG database. Differentially expressed genes (DEGs were identified using log10 values with the FPKM method. Among 5,167DEGs, 491 genes were differentially expressed at freezing temperature compared to the control temperature in both CT and CS libraries. The DEG results were validated with qRT-PCR. We performed GO and KEGG pathway enrichment analyses of all DEGs and iPath interactive analysis found 31 pathways including those related to metabolism of carbohydrate, nucleotide, energy, cofactors and vitamins, other amino acids and xenobiotics biodegradation. Furthermore, a large number of molecular markers were identified from the assembled genes, including simple sequence repeats (SSRs 4,437 and SNP substitutions of transition and transversion types of CT and CS. Our study is the first to provide a transcriptome sequence resource for Allium spp. with regard to cold and freezing stress. We identified a large set of genes and determined their DEG profiles under cold and freezing conditions using two different genotypes. These data represent a valuable resource for genetic and genomic studies of Allium spp.

  17. Plant odours with potential for a push-pull strategy to control the onion thrips (Thrips tabaci)

    NARCIS (Netherlands)

    Tol, van R.W.H.M.; James, D.E.; Kogel, de W.J.; Teulon, D.A.J.

    2007-01-01

    We compared the efficacy of four plant essential oils to repel onion thrips, Thrips tabaci (Lindeman) (Thysanoptera: Thripidae), in the presence of an attractive odour, ethyl iso-nicotinate in a pasture field. Four horizontal white sticky plates were placed adjacent to (directions: N, S, E, W) a

  18. Evaluating the Effect of Onion (Allium cepa L. Sowing Methods on Growth Characteristics of Purple Nutsedge (Cyperus rotundus L. under Different Levels of Nitrogen Fertilizer

    Directory of Open Access Journals (Sweden)

    Nosratollah Karimi Arpnahy

    2017-03-01

    Full Text Available Introduction: Increasing crop competitive ability is an important part of integrated weed management (IWM. In this regard, identifying weeds reaction to soil fertility status for developing fertilizing methods, as components of IWM programs, is a necessity. Materials and Methods: In order to evaluate the growth characteristics of purple nutsedge under the conditions of interference with onion, a factorial experiment based on randomized complete block design was conducted with three replications at the Research Greenhouse of University of Birjand in 2013. The first factor included three sowing methods of onion (seed sowing, onion set and transplanting and the second factor included three levels of nitrogen (50, 100 and 150 kg N ha-1, equivalent to 25, 50 and 75 mg N kg-1 soil, urea fertilizer with a purity of 46% was used for this purpose. Results and Discussion: The results of analysis of variance showed that sowing method and nitrogen levels had significant effects on plant height, leaf area, aboveground dry weight as well as tuber number and underground dry weights. Moreover, the interaction between sowing methods and nitrogen levels had a significant effect on plant height, leaf area, aboveground dry weight as well as tuber number and underground dry weights. The results of the comparisons of the mean for the interaction of planting methods and different levels of nitrogen indicated an increased weeds growth in direct seeding method and high levels of nitrogen in weed height trait, so that the greatest nutsedge height during the growing season (92.33 cm was obtained at direct seeding method and high nitrogen levels . Moreover, the lowest weed height during the growing season was obtained at onion set planting method and 50 kg ha-1 nitrogen. The highest leaf area of purple nutsedge at ten weeks after planting (446.65 cm2, was obtained in direct seeding method and high levels of nitrogen, and the lowest value for this trait (203.38 cm2, was

  19. INFLUENCE OF CULTIVARS AND SEED THERMAL TREATMENT ON THE DEVELOPMENT OF FUNGAL PATHOGENS IN CARROT AND ONION PLANTS

    Czech Academy of Sciences Publication Activity Database

    Koudela, M.; Novotný, Čeněk

    2016-01-01

    Roč. 64, č. 4 (2016), s. 1181-1189 ISSN 1211-8516 R&D Projects: GA MZe QJ1210165 Institutional support: RVO:61388971 Keywords : carrot * onion * fungal pathogens * plants infection Subject RIV: EE - Microbiology, Virology

  20. A safe potential juice clarifying pectinase from Trichoderma viride EF-8 utilizing Egyptian onion skins

    Directory of Open Access Journals (Sweden)

    Abdel-Mohsen S. Ismail

    2016-06-01

    Full Text Available The production of a notable, safe and highly active pectinase by the local fungal strain Trichoderma viride EF-8 utilizing the abundant pigmented Egyptian onion (Allium cepa L. skins (6.5%, w/v was achieved in 4 days submerged fermentation (SMF cultures, at temperature and pH of 30 °C and 4.0, respectively. The indigenously produced pectinase was partially purified by 50% batch ethanol precipitation and its general properties were studied following the standard procedures. The lyophilized enzyme preparation was free of any ochra or aflatoxins. The optimum conditions for the partially purified enzyme form were 2 mg/mL and 1% (w/v enzyme protein and substrate (citrus pectin concentrations, reaction pH and temperature of 7.0 and 40 °C, respectively. The results presented the low cost onion skins waste as the major substrate for the fungal pectinase production and its subsequent use in perfect fruit (apple, lemon and orange juices clarification with remarkable stability during and after this process, which certainly enhance fruit juices processing in the tropics.

  1. Accelerated aging and controlled deterioration for the determination of the physiological potential of onion seeds

    Directory of Open Access Journals (Sweden)

    Rodo Angelica Brod

    2003-01-01

    Full Text Available International research on vegetable seed vigor is not at the same level attained for grain crops species. This study was conducted to identify reliable procedures for the accelerated aging and controlled deterioration tests to rank onion (Allium cepa L. seed lots according to their physiological potential. Six seed lots of the cultivars Aurora and Petroline were evaluated in the laboratory for germination, first count, seedling vigor classification, traditional and saturated salt accelerated aging (41masculineC / 48 and 72 h, controlled deterioration (24% of water / 45masculineC / 24 h and seedling emergence tests. Seed moisture content after the saturated salt accelerated aging test was lower and uniform, which is considered an important advantage in comparison to the traditional procedure. The saturated salt accelerated aging (41masculineC / 48 and 72 h and controlled deterioration (moisture content adjusted to 24% / 45masculineC / 24 h tests were the best procedures to assess the physiological potential of onion seeds, and are indicated for use in quality control programs.

  2. Morphological, nutritional and chemical description of "Vatikiotiko", an onion local landrace from Greece.

    Science.gov (United States)

    Petropoulos, Spyridon A; Fernandes, Ângela; Barros, Lillian; Ferreira, Isabel C F R; Ntatsi, Georgia

    2015-09-01

    "Vatikiotiko" is an onion local landrace from Greece with special quality features, such as strong and pungent taste and storability. In this study, we tried for the first time to describe this landrace and record its morphological traits and nutritional value in comparison with commercially cultivated genotypes ("Creamgold", "Red Cross F1" and "Sivan F1"). Nutritional value of "Vatikiotiko" was higher than commercial genotypes, whereas total sugars, fructose and glucose content were lower in "Vatikiotiko" and "Creamgold" onions. Fatty acids composed mostly from polyunsaturated fatty acids (linoleic and α-linolenic acid), whereas for "Vatikiotiko" saturated and monounsaturated fatty acids were detected in equal amounts (29.79% and 30.60%, respectively). "Vatikiotiko" and "Creamgold" had low antioxidant activity (DPPH radical scavenging activity), especially when comparing with "Sivan F1" and "Red Cross F1", whereas no flavonoids were detected in "Vatikiotiko". The overview of "Vatikiotiko" landrace supported its special character regarding its nutritional value (sugar content, mineral composition and fatty acids profile) and the necessity to revalorize and protect its traditional culture. The potential of introducing it as Protected Designation of Origin (PDO) or Protected Geographical Indication (PGI) product has also been discussed. Copyright © 2015 Elsevier Ltd. All rights reserved.

  3. Timing of glyphosate applications to wheat cover crops to reduce onion stunting caused by Rhizoctonia solani

    Science.gov (United States)

    Stunting caused by Rhizoctonia spp. is economically important in irrigated onion bulb crops in the semi-arid Columbia Basin of Oregon and Washington, where cereal winter cover crops commonly are planted the previous fall to prevent wind erosion of soil. The cover crop is killed with herbicide applic...

  4. White rot of garlic and onion (Causal agent, Sclerotium cepivorum): A status report from the Pacific Northwest

    Science.gov (United States)

    There is evidence from literature, state department of agriculture documents, and recent diagnoses that Sclerotium cepivorum, causal agent of white rot of garlic and onion, is spreading and/or becoming more established in the Pacific Northwest. Previously documented distributions are summarized, an...

  5. The root systems of onion and Allium fistulosum in the context of organic farming: a breeding approach

    NARCIS (Netherlands)

    Melo, de P.

    2003-01-01

    Keywords: Allium cepa , Allium roylei , Japanese bunching onion, introgression breeding, organic agriculture, linkage map, AFLP, QTL analysis, Arbuscular Mycorrhizal Fungi, in vitro multiplication

    Towards an improved variety assortment for the Dutch organic sector : case studies on onion and spring wheat

    NARCIS (Netherlands)

    Osman, A.M.

    2014-01-01

    Key words:

    organic farming; principles of organic agriculture; food production chain;

    plant breeding; genetic correlation; plant traits; farmers’ preferences;

    variety testing; Value for Cultivation and Use; EU seed legislation;

    onion; Allium cepa; spring wheat;

  6. Upregulation of BAG3 with apoptotic and autophagic activities in maggot extract‑promoted rat skin wound healing.

    Science.gov (United States)

    Dong, Jian-Li; Dong, Hai-Cao; Yang, Liang; Qiu, Zhe-Wen; Liu, Jia; Li, Hong; Zhong, Li-Xia; Song, Xue; Zhang, Peng; Li, Pei-Nan; Zheng, Lian-Jie

    2018-03-01

    Maggot extract (ME) accelerates rat skin wound healing, however its effect on cell maintenance in wound tissues remains unclear. B‑cell lymphoma (Bcl) 2‑associated athanogene (BAG)3 inhibits apoptosis and promotes autophagy by associating with Bcl‑2 or Beclin 1. Bcl‑2, the downstream effector of signal transducer and activator of transcription 3 signaling, is enhanced in ME‑treated wound tissues, which may reinforce the Bcl‑2 anti‑apoptotic activity and/or cooperate with Beclin 1 to regulate autophagy during wound healing. The present study investigated expression levels of BAG3, Bcl‑2, Beclin 1 and light chain (LC)3 levels in rat skin wound tissues in the presence and absence of ME treatment. The results revealed frequent TUNEL‑negative cell death in the wound tissues in the early three days following injury, irrespective to ME treatment. TUNEL‑positive cells appeared in the wound tissues following 4 days of injury and 150 µg/ml ME efficiently reduced apoptotic rate and enhanced BAG3 and Bcl‑2 expression. Elevated Beclin 1 and LC3 levels and an increased LC3 II ratio were revealed in the ME‑treated tissues during the wound healing. The results of the present study demonstrate the anti‑apoptotic effects of BAG3 and Bcl‑2 in ME‑promoted wound healing. Beclin 1/LC3 mediated autophagy may be favorable in maintaining cell survival in the damaged tissues and ME‑upregulated BAG3 may enhance its activity.

  7. Seasonal dynamics of thrips (Thrips tabaci) (Thysanoptera: Thripidae) transmitters of iris yellow spot virus: a serious viral pathogen of onion bulb and seed crops.

    Science.gov (United States)

    Bag, Sudeep; Rondon, Silvia I; Druffel, Keri L; Riley, David G; Pappu, Hanu R

    2014-02-01

    Thrips-transmitted Iris yellow spot virus (IYSV) is an important economic constraint to the production of bulb and seed onion crops in the United States and many other parts of the world. Because the virus is exclusively spread by thrips, the ability to rapidly detect the virus in thrips vectors would facilitate studies on the role of thrips in virus epidemiology, and thus formulation of better vector management strategies. Using a polyclonal antiserum produced against the recombinant, Escherichia coli-expressed nonstructural protein coded by the small (S) RNA of IYSV, an enzyme linked immunosorbent assay was developed for detecting IYSV in individual as well as groups of adult thrips. The approach enabled estimating the proportion of potential thrips transmitters in a large number of field-collected thrips collected from field-grown onion plants. Availability of a practical and inexpensive test to identify viruliferous thrips would be useful in epidemiological studies to better understand the role of thrips vectors in outbreaks of this economically important virus of onion.

  8. Marigold (Tagetes erecta L. as an attractive crop to natural enemies in onion fields Cravo-de-defunto (Tagetes erecta L. como cultura atrativa para inimigos naturais em cultivo de cebola

    Directory of Open Access Journals (Sweden)

    Luís Cláudio Paterno Silveira

    2009-12-01

    Full Text Available Onion is the third most grown vegetable crop in São Paulo state, Brazil. Organic onion farming is expected to increase in the state due to the increasing demand. Pest management in organic onion farming is based on plant extracts with insecticide effects. However, the efficacy of such plant extracts has not been proved yet, and it was observed that they do negatively affect natural enemies. Plants surrounding onion fields, and that are attractive to natural enemies, may be a good option to farmers, since they may lead to increased diversity of arthropod species and, consequently, the natural control of pest populations. This study deals with the effect of marigold plants as a resource plant to natural enemies in onion fields. The experiment was set in a certified organic farm using marigold rows at a center of an onion field. Samples were taken from marigold and the onion plants 5 m (near and 30 m (far from the flowering strips. Higher numbers of arthropod pests were observed in onion plants 30 m from the marigold strip, while higher numbers of predators and parasitoids were found at 5 m distance. Species richness and Shannon's diversity index were higher at 5 m from marigold. Therefore, marigold rows next to onion fields resulted in higher number of entomophagous species, potentially enhancing the natural control of onion pests. In the study field, marigold strips may be an alternative to crop sprays for organic control of onion pests.A cebola é a terceira hortaliça mais cultivada em São Paulo, cujo cultivo orgânico tende a crescer devido ao grande mercado consumidor existente. O manejo das pragas na cebolicultura orgânica baseia-se nos extratos de plantas inseticidas que, além de terem eficiência não comprovada, podem afetar negativamente os inimigos naturais. A utilização de plantas atrativas a inimigos naturais no entorno dos cultivos de cebola pode ser uma boa opção para os produtores, pois potencialmente aumentam a diversidade

  9. Development and validation of a real-time quantitative PCR assay to detect Xanthomonas axonopodis pv. allii from onion seed.

    Science.gov (United States)

    Robène, Isabelle; Perret, Marion; Jouen, Emmanuel; Escalon, Aline; Maillot, Marie-Véronique; Chabirand, Aude; Moreau, Aurélie; Laurent, Annie; Chiroleu, Frédéric; Pruvost, Olivier

    2015-07-01

    Bacterial blight of onion is an emerging disease threatening world onion production. The causal agent Xanthomonas axonopodis pv. allii is seed transmitted and a reliable and sensitive tool is needed to monitor seed exchanges. A triplex quantitative real-time PCR assay was developed targeting two X. axonopodis pv. allii-specific markers and an internal control chosen in 5.8S rRNA gene from Alliaceae. Amplification of at least one marker indicates the presence of the bacterium in seed extracts. This real-time PCR assay detected all the 79 X. axonopodis pv. allii strains tested and excluded 85.2% of the 135 non-target strains and particularly all 39 saprophytic and pathogenic bacteria associated with onion. Cross-reactions were mainly obtained for strains assigned to nine phylogenetically related X. axonopodis pathovars. The cycle cut-off was estimated statistically at 36.3 considering a risk of false positive of 1%. The limit of detection obtained in at least 95% of the time (LOD 95%) was 5×10(3) CFU/g (colony forming unit/g). The sensitivity threshold was found to be 1 infected seed in 32,790 seeds. This real-time PCR assay should be useful for preventing the long-distance spread of X. axonopodis pv. allii via contaminated seed lots and determining the epidemiology of the bacterium. Copyright © 2015 Elsevier B.V. All rights reserved.

  10. Suppressing immature house and stable flies in outdoor calf hutches with sand, gravel, and sawdust bedding.

    Science.gov (United States)

    Schmidtmann, E T

    1991-11-01

    Sand, gravel, sawdust, and pine shavings were used as bedding in outdoor calf hutches and compared with straw relative to the density of immature (maggot) house flies, Musca domestica, and stable flies, Stomoxys calcitrans. In 6-wk field trials, average densities of house and stable fly maggots in concrete mix sand ranged from only .3 to 1.6 and 0 to .1 maggots/L, respectively; pea size gravel bedding also strongly suppressed densities from less than .1 to .3 and less than .1 to .1 maggots/L, respectively. These densities represent reductions of 76 to greater than 99% relative to straw bedding, but both sand and gravel compacted and became soiled with calf feces, which resulted in unacceptable bedding sanitation and foul odors. Densities of house and stable fly maggots in pine shavings did not differ from those in straw bedding. Nevertheless, in sawdust bedding, maggot density was limited to averages of 1.4 to 8.3 house and 9.8 to 11.8 stable fly maggots/L; this represented reductions of 45 to 91% relative to straw. In a follow-up trial, house and stable fly maggot densities in sawdust averaged 11.3 and 43.9 maggots/L, respectively, reductions of 77 and 46%. These findings suggest that bedding calf hutches with sawdust during warm weather can be useful as an ecologically sound approach to controlling muscoid fly populations on dairy farms.

  11. The Raman spectrum of nano-structured onion-like fullerenes

    International Nuclear Information System (INIS)

    Wang Xiaomin; Xu Bingshe; Liu Xuguang; Jia Husheng; Hideki, Ichinose

    2005-01-01

    Onion-like fullerenes (OLFs) have been studied by scanning electron microscopy (SEM), high resolution transmission electron microscopy (HRTEM) and Raman spectroscopy. With a precise control of current, high-quality OLFs with few defects are found on the redeposit rod on the cathode. The size of OLFs is found to be in the range of 15-40 nm. Metal particles are useful support crystal to realize the synthesis of high-quality OLFs. Raman spectra of OLFs show high degree of graphitization. Compared with that of highly oriented prolific graphite (HOPG), the strain of graphene planes due to curvature and uneven distribution of the diameter of OLFs has been estimated analytically and is used to account for the downward shift of the G peak

  12. The effect of cultivar, sowing date and transplant location in field on bolting of Welsh onion (Allium fistulosum L.).

    Science.gov (United States)

    Dong, Yinxin; Cheng, Zhihui; Meng, Huanwen; Liu, Hanqiang; Wu, Cuinan; Khan, Abdul Rehman

    2013-10-07

    Bolting reduces the quality and commercial yield of Welsh onion (Allium fistulosum L.) in production. However, seed production is directly dependent on flower induction and bolting. The Welsh onion belongs to the green plant vernalisation type, specific seedling characteristics and sufficient accumulated time at low temperature are indispensible for the completion of its vernalisation process. Only if these conditions for vernalisation are fulfilled, the plants will bolt in the following year. The present investigation evaluated the effects of cultivar, sowing date and transplant location in field on the bolting of Welsh onion at the Horticultural Farm of the College of Horticulture, Northwest A&F University, Yangling, Shannxi Province, China in two succeeding production years: 2010-2011 and 2011-2012. A strip split plot layout within a randomised complete block design with three replications was used. The results revealed that all three factors (cultivar, sowing date and transplant location) and their interaction had significant effects on the initiation and final rate of bolting observed by 30 April. The earliest bolting date (14 February, 2011 and 15 February, 2012) and the highest bolting rate (100% in 2011 and 62% in 2012) occurred when the JinGuan cultivar was sown on 20 August and transplanted in a plastic tunnel, whereas the latest date and lowest rate (no bolting observed until 30 April) of bolting occurred when the XiaHei cultivar was sown on 29 September and transplanted in an open field. These results suggest that we can control bolting in Welsh onion production by choosing an appropriate cultivar, sowing date and transplant location. Choosing a late bolting cultivar, such as cultivar XiaHei, sowing around October, and transplanting in the open field can significantly delay bolting, while a sowing date in late August should be selected for seed production, and the seedlings should be transplanted in a plastic tunnel to accelerate development of the

  13. Efeito de preparados caseiros no controle da queima-acinzentada, na cultura da cebola Alternative formulations for control of Botrytis squamosa on onion

    Directory of Open Access Journals (Sweden)

    Pedro Boff

    1999-07-01

    Full Text Available Agricultores do Alto Vale do Itajaí (SC, vem utilizando preparados caseiros no tratamento fitossanitário da cebola, cuja principal doença, durante a fase de muda, é a queima-acinzentada, causada pelo fungo Botrytis squamosa. Neste trabalho, realizado nos anos de 1993, 94 e 95, no período de muda da cebola, durante a fase de canteiro, avaliou-se o efeito de extratos alcóolicos de Matricaria chamomilla, Mentha glacilis, alho e própolis; cinza vegetal e cal; soluções de B+Zn, KMnO4, NaClO, Sílica-gasil, creolina, formalina; calda sulfocálcica, calda bordalesa, cânfora, enxofre, enxofre+silicato+propólis e o fungo antagonista, Gliocladium roseum, sobre o desenvolvimento da queima-acinzentada. A aplicação dos produtos foi semanal e as concentrações foram ajustadas em ensaio preliminar. O estudo de eficiência mostrou que Gliocladium roseum (108 esporos/ml igualou-se ao fungicida vinclozolin (0,075%, no controle da queima-acinzentada em 1994/95. No ciclo de 1995/96, maior sobrevivência de plantas e/ou maior número de plantas aptas ao transplante foram obtidos nos tratamentos com cinza vegetal, calda bordalesa (0,5% e a mistura de enxofre + silicato + extrato de própolis, comparados com o tratamento padrão, com fungicida. As soluções de KMnO4, NaClO, cânfora, enxofre e sílica-gasil não mostraram diferença com a testemunha não tratada. A adubação orgânica de base, em 1995, proporcionou mudas com menor intensidade de B. squamosa e maior índice de sobrevivência das mesmas do que adubação mineral, independentemente da formulação.Santa Catarina State farmers in the "Alto Vale do Itajaí" region are using several home-made products to control onion pests, where leaf blight of onion (Botrytis squamosa is the main nursery disease. This research was carried out during the nursery period in 1993, 94 and 95, in order to evaluate traditional formulations against leaf blight of onion. The formulations studied were alcoholic

  14. Genotypic variation in the sulfur assimilation and metabolism of onion (Allium cepa L.) I. Plant composition and transcript accumulation

    KAUST Repository

    McCallum, John A.; Thomas, Ludivine; Shaw, Martin L.; Pither-Joyce, Meeghan D.; Leung, Susanna; Cumming, Mathew; McManus, Michael T.

    2011-01-01

    Organosulfur compounds are major sinks for assimilated sulfate in onion (Allium cepa L.) and accumulation varies widely due to plant genotype and sulfur nutrition. In order to better characterise sulfur metabolism phenotypes and identify potential

  15. Comparison of the Main Bioactive Compounds and Antioxidant Activities in Garlic and White and Red Onions after Treatment Protocols

    Czech Academy of Sciences Publication Activity Database

    Gorinstein, S.; Leontowich, H.; Leontowicz, M.; Namiesnik, J.; Najman, K.; Drzewiecki, J.; Cvikrová, Milena; Martincová, Olga; Katrich, E.; Trakhtenberg, S.

    2008-01-01

    Roč. 56, č. 12 (2008), s. 4418-4426 ISSN 0021-8561 Institutional research plan: CEZ:AV0Z50380511 Keywords : Garlic * onions * bioactive compounds Subject RIV: EF - Botanics Impact factor: 2.562, year: 2008

  16. Strip Tillage and Early-Season Broadleaf Weed Control in Seeded Onion (Allium cepa)

    OpenAIRE

    Sarah Gegner-Kazmierczak; Harlene Hatterman-Valenti

    2016-01-01

    Field experiments were conducted in 2007 and 2008 near Oakes, North Dakota (ND), USA, to evaluate if strip tillage could be incorporated into a production system of seeded onion (Allium cepa) to eliminate the standard use of a barley (Hordeum vulgare) companion crop with conventional, full width tillage, yet support common early-season weed control programs. A split-factor design was used with tillage (conventional and strip tillage) as the main plot and herbicide treatments (bromoxynil, DCPA...

  17. Effects of Different Mordants on Silk Fabric Dyed with Onion Outer Skin Extracts

    OpenAIRE

    Mohammad Gias Uddin

    2014-01-01

    At present, a higher demand is put towards the use of natural dyes due to increased awareness of the environmental and health hazards associated with the synthesis and use of synthetic dyes. This research was conducted using onion outer skins as a potential source of natural plant dyes. In this study, extraction of dye was carried out in aqueous boiling method. Premordanting technique was followed using different mordants, namely alum, ferrous sulphate, tin, tannic acid, tartaric acid, and th...

  18. Dyeing of γ-irradiated cotton with natural flavonoid dye extracted from irradiated onion shells (Allium cepa) powder

    Science.gov (United States)

    Rehman, Fazal-ur; Adeel, Shahid; Shahid, Muhammad; Bhatti, Ijaz Ahmad; Nasir, Faiza; Akhtar, Nasim; Ahmad, Zulfiqar

    2013-11-01

    Powder of Onion shells as a source of natural flavonoid dye (Quercetin) and cotton fabrics were exposed to absorbed doses of 2, 4, 6, 8 and 10 kGy using Cs-137 gamma irradiator. Irradiated and un-irradiated dye powder was used for extraction of quercetin as well as antibacterial, hemolytic and antioxidant activities were also determined to observe the effect of radiation. Furthermore, color strength and colourfastness of irradiated fabrics were improved by using pre and post-mordants such as alum and iron. It is found that 4 kGy is the optimal absorbed dose for extraction of natural quercetin extracted from onion shells while maximum color strength and acceptable fastness properties are obtained on dyeing of irradiated fabric at 60 °C keeping M:L of 1:30 using 10% alum as pre-mordant and 6% alum as post-mordant. Gamma irradiation has not only improved the color strength of the dye using irradiated cotton but also that of colourfastness properties.

  19. The Commanding Officer’s Perspective on Protecting Shipboard IT Networks

    Science.gov (United States)

    2014-09-01

    38 John Markus and Paul J. DeLia, “Jamming,” AccessScience , accessed March 22, 2014, http://accessscience.com/content/Jamming/358300. 39 Sean M...www.infoworld.com/d/security-central/fbi-worried-DOD-sold-counterfeit- cisco-gear-266. Markus, John and Paul J. DeLia. “Jamming.” AccessScience

  1. Low levels of H2S may replace sulfate as sulfur source in sulfate-deprived onion

    NARCIS (Netherlands)

    Durenkamp, Mark; De Kok, LJ

    2005-01-01

    Onion (Allium cepa L.) was exposed to low levels of H2S in order to investigate to what extent H2S could be used as a sulfur source for growth under sulfate-deprived conditions. Sulfate deprivation for a two-week period resulted in a decreased biomass production of the shoot, a subsequently

  2. The importance of using the irradiation technology in the post-harvest Preservation of onions and garlic

    International Nuclear Information System (INIS)

    Iglesias Enriquez, Isora

    1999-01-01

    In Cuba post-harvest preservation of onions and garlic for different uses have been performed by irradiation bulbs with a minimal dose range of 80 to 90 Gy of Gamma radiation (Co60 ) at commercial level in the Food Irradiation Plant (PIA) Producto 1 which in 1986 held a nominal activity of 110 000 ci. Results showed that the irradiated products could be preserved up to 8 and 11 months, respectively, resulting un total losses lower than 30 %. Products were stored in a warehouse with forced air distribution system of 22 0C to 32 0C and 70 to 100 % RH, resulting in 30 air changes /hour. An important economic benefit was obtained from this method as compared to other traditional storage methods using controlled temperature chambers ( 1 0C to 3 0C ) to preserve un-irradiated onions an garlic's. It is concluded that the irradiated products could be stored at atmospheric temperature and forced air distribution system resulting in lower losses and energy savings and non-imported product, which to reached more of the 5 dollars millions

  3. Enhanced rate capability and cycling stability of core/shell structured CoFe{sub 2}O{sub 4}/onion-like C nanocapsules for lithium-ion battery anodes

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Xianguo, E-mail: liuxianguohugh@gmail.com [School of Materials Science and Engineering, Anhui University of Technology, Maanshan 243002 (China); Wu, Niandu; Cui, Caiyun; Zhou, Pingping [School of Materials Science and Engineering, Anhui University of Technology, Maanshan 243002 (China); Sun, Yuping [Center for Engineering Practice and Innovation Education, Anhui University of Technology, Maanshan 243032 (China)

    2015-09-25

    Highlights: • Core/shell-structured CoFe{sub 2}O{sub 4}/onion-like carbon nanocapsules have been prepared. • CoFe{sub 2}O{sub 4}/C nanocapsules possess good reversibility even when the current density is up to 4C. • CoFe{sub 2}O{sub 4}/C nanocapsules obtain a discharge capacity of 914.2 mA h g{sup −1} after 500 cycles at 0.1C. - Abstract: In this work, core/shell structured CoFe{sub 2}O{sub 4}/onion-like C nanocapsules have been successfully fabricated by the arc discharge method and air-annealing process and confirmed by X-ray diffraction and high-resolution transmission electron microscopy. The core/shell structure effectively withstands the volume change of CoFe{sub 2}O{sub 4} nanoparticles during the cycling process. Moreover, the onion-like C shells reduce the charge transfer resistance and facilitate electron and ion transport throughout the electrode. As a result, CoFe{sub 2}O{sub 4}/onion-like C nanocapsules exhibit excellent performance as a potential anode material for lithium ion batteries and deliver a reversible capacity of 914.2 mA h g{sup −1} at 0.1C, even after 500 cycles and recover its original capacity when the rate returns from 4C to the initial 0.1C after 120 cycles.

  4. The Burkholderia cepacia rpoE gene is not involved in exopolysaccharide production and onion pathogenicity.

    Science.gov (United States)

    Devescovi, Giulia; Venturi, Vittorio

    2006-03-01

    Burkholderia cepacia was originally described as the causative agent of bacterial rot of onions, and it has now emerged as an important opportunistic pathogen causing severe chronic lung infections in patients having cystic fibrosis. Burkholderia cepacia is now classified into nine very closely related species (previously designated as genomovars), all of which have been isolated from both environmental and clinical sources and are collectively known as the B. cepacia complex. The alternative extracytoplasmic function sigma factor, sigmaE, has been determined in several bacterial species as making substantial contributions to bacterial survival under stress conditions. Here, we report the identification and characterization of the rpoE gene, encoding sigmaE, of B. cepacia. It is highly similar to sigmaE of other bacteria, including Escherichia coli and Pseudomonas aeruginosa. Studies using an rpoE knockout mutant of B. cepacia revealed that many stress adaptations, including osmotic, oxidative, desiccation, carbon, and nitrogen stress, were independent of sigmaE. Similarly, biofilm formation; production of exopolysaccharides, N-acyl homoserine lactones, and several exoenzymes; and onion pathogenicity were not affected by the absence of sigmaE. In contrast, sigmaE contributed to the adaptation to heat stress and phosphate starvation.

  5. Cool-weather activity of the forensically important hairy maggot blow fly Chrysomya rufifacies (Macquart) (Diptera: Calliphoridae) on carrion in Upstate South Carolina, United States.

    Science.gov (United States)

    Cammack, Jonathan A; Nelder, Mark P

    2010-02-25

    The hairy maggot blow fly Chrysomya rufifacies (Macquart) (Diptera: Calliphoridae) has expanded its range in the United States since its introduction into Texas (ca. 1980) and has been collected in 15 states. We investigated the bionomics of immature and adult C. rufifacies collected from carcasses of a raccoon Procyon lotor (Linnaeus) and white-tailed deer Odocoileus virginianus Zimmerman in Upstate South Carolina during November 2007, and used these insects to estimate the minimum period of insect activity. Puparia of C. rufifacies were collected from deer carrion; 28% were parasitized by Nasonia vitripennis (Walker) (Hymenoptera: Pteromalidae). The mean daily ambient temperature during this study was 11.4+/-1.02 degrees C, representing the lowest recorded mean temperature for adult activity of C. rufifacies; adults of C. rufifacies were observed flying among the carcasses at 9.0 degrees C. Although C. rufifacies is considered a warm-weather blow fly, researchers should be aware of its activity at suboptimal conditions, behavior that might aid its expansion into more northern areas. 2009 Elsevier Ireland Ltd. All rights reserved.

  6. Evaluation of the quality of hot air dehydrated onion coming from gamma radiated bulbs; Evaluacion de la calidad de cebolla deshidratada por aire caliente proveniente de bulbos tratados con radiacion gamma

    Energy Technology Data Exchange (ETDEWEB)

    Elman, L; Pezzutti, A; Croci, C A [Universidad Nacional del Sur, Bahia Blanca (Argentina). Laboratorio de Radioisotopos

    2003-07-01

    The purpose of this work was to evaluate the quality of hot air dehydrated onion, as regards physical and chemical characteristics, coming from the regional product that was gamma irradiated for sprout inhibition. We worked with the onion variety Valenciana Sintetica 14. Radio inhibition was made 30 days post harvest with gamma radiation from a {sup 60}Co source at the Centro Atomico Ezeiza-CNEA, using a dose of 60 Gy. The skin of the bulbs was manually removed and the bulbs were cut in pieces 3 mm thick and between 1 and 3 cm long. The material was dehydrated in a rotating dryer with forced air circulation at 60 C degrees, between 0.8 and 1.7 m/s air speed and at ambient relative humidity. Dehydration was made 80 days after post-irradiation. The quality of the dehydrated onion was evaluated by the following physical- chemical analysis: total solids content, pungency (indirectly measured by pyruvic acid content assessment), color, pH, carbon hydrates and sensorial analysis. All analytical determinations were made in triplicate. The results obtained showed there are no significant changes between the averages of the physical-chemical properties of the control dehydrated samples and those coming from the radio-inhibited raw matter. According to the sensorial analysis, only the color of dehydrated onion was affected by the radio inhibition process. However, and according to the panel members comment, the greatest browning degree observed in ionizing radiation treated onion seemed to result more attractive to them. It may be concluded that radio inhibited regional onion can be useful as raw matter for hot air dehydrated product. It must be remarked that its use would extend the product use by dehydration plants, thus implying an increase of their processing capacity with the corresponding financial benefit. (author)

  7. Order of 21 June 1984 on trade in garlic, onions and shallots treated by ionizing radiation

    International Nuclear Information System (INIS)

    1984-01-01

    This Order sets the licensing conditions for possession with a view to the sale and putting on sale of onion, garlic and shallot bulbs whose germination has been inhibited by exposure to cobalt 60 or caesium 137 gamma radiation or to accelerated electron beams with an energy below or equal to 10 million electron-volts. (NEA) [fr

  8. Addition of a combination of onion (Allium cepa) and coconut (Cocos nucifera) to food of sheep stops gastrointestinal helminthic infections.

    Science.gov (United States)

    Mehlhorn, Heinz; Al-Quraishy, Saleh; Al-Rasheid, Khaled A S; Jatzlau, Antje; Abdel-Ghaffar, Fathy

    2011-04-01

    Sheep with gastrointestinal nematodes and cestodes were fed on three farms with a combination of specially prepared extracts of onion (Allium cepa) and coconut (Cocos nucifera) for 8 days containing each 60 g coconut and onion extract, combined with milk powder and/or polyethylene glycol (PEG) propylencarbonate (PC). In all cases, the worm stages disappeared from the feces and were also not found 9 and 20 days after the end of the feeding with this plant combination. Since all treated animals increased their body weight considerably (when compared to untreated animals), worm reduction was apparently as effective as it was shown in previous laboratory trials with rats and mice (Klimpel et al., Parasitol Res, in press, 2010; Abdel-Ghaffar et al., Parasitol Res, in press, 2010; in this volume).

  9. Carbon nano-onions as fluorescent on/off modulated nanoprobes for diagnostics

    Directory of Open Access Journals (Sweden)

    Stefania Lettieri

    2017-09-01

    Full Text Available Multishell fullerenes, known as carbon nano-onions (CNOs, have emerged as a platform for bioimaging because of their cell-penetration properties and minimal systemic toxicity. Here, we describe the covalent functionalization of CNOs with a π-extended distyryl-substituted boron dipyrromethene (BODIPY dye with on/off modulated fluorescence emission activated by an acidic environment. The switching properties are linked to the photoinduced electron transfer (PET characteristics of the dimethylamino functionalities attached to the BODIPY core. The on/off emission of the fluorescent CNOs is fast and reversible both in solution and in vitro, making this nanomaterial suitable as pH-dependent probes for diagnostic applications.

  10. Study of optimal operation for producing onion vinegar using two continuously stirred tank reactors

    OpenAIRE

    小林, 秀彰; 山口, 文; 富田, 弘毅; 管野, 亨; 小林, 正義; KOBAYASHI, Hideaki; YAMAGUCHI, Kazaru; TOMITA, Koki; KANNO, Tohru; KOBAYASHI, Masayoshi

    1997-01-01

     Onion vinegar was produced using a 2-stage continuously stirred tank reactor. Regarding the alcohol fermentation and the acetic acid fermentation examined in this study, the immobilized cells on porous ceramics offered stable production of alcohol and acetic acid for long periods of 300 and 700 days, respectively. Compared with the steady-state operation method, the temperature-change forced-cyclic operation method increased ethanol yield of alcohol fermentation by a maximum of 15%. Acetic a...

  11. Studies on sprout inhibition of potatoes and onions and shelf-life extensions of dates in Iraq

    International Nuclear Information System (INIS)

    Auda, H.; Khalaf, Z.

    1979-01-01

    Small scale experiments were carried out with onions and potatoes to examine the effect of gamma irradiation on sprout inhibition under different storage conditions. Doses of 15 to 20 krad caused some physical damage to potatoes stored in an incubator at 4 to 10 0 C and 40 to 70% relative humidity. This damage was also observed when potatoes have been stored at room temperature. Sprouting appeared after 30 to 60 days storage. The best results were obtained when a dose of 10 to 12 krad was used. Studies on onions irradiated and stored at room temperature for ten months showed that 6 to 9 krad gave the best results, extending the storage for 10 months. No sprouting could be detected throughout the storage period. Shelf-life and organoleptic study was done on four varieties of Iraqi dates at different stages of development. The results showed that the eating quality of fresh dates was not affected significantly by irradiation up to 270 krad and the duration of softening process (after-ripening) of dates was prolonged by low doses of 10 to 90 krad in the majority of the experimental batches. (author)

  12. Macroscopic and microscopic effects of gamma radiation on the shallot onions, Allium cepa var. aggregatum

    International Nuclear Information System (INIS)

    Medina, V.F.O.

    1995-01-01

    The document is a study on the relationship between irradiation dose and the macroscopic and microscopic parameters and chromosomal aberrations in the onions. The data were analyzed using analysis of variance or F-test to determine significant differences among treatments as affected by does of radiation followed by Duncan's Multiple Range Test (DMRT). LSD test was also used in comparing means when the F-ratio was significant. 23 refs.; 19 figs.; tabs

  13. Oral Myiasis Caused by Cochliomyia hominivorax in a Disabled Person

    Directory of Open Access Journals (Sweden)

    José Pereira Novo-Neto

    2015-01-01

    Full Text Available Myiasis is a parasitic disease caused by developing maggots of fly species, which can infect humans. Patients with special needs, especially those with severe neuropsychomotor limitations, may have oral manifestations of this disease. Here, we present a clinical case in which a disabled person was affected by oral myiasis caused by Cochliomyia hominivorax. Maggots were found in two ulcerated lesions, a 2 cm diameter lesion in the maxilla and a 6 cm diameter lesion in the mandible. Forty-five maggots were removed during inspection, whereas 75 maggots were surgically removed under general anesthesia with nasotracheal intubation. Dipyrone, ivermectin, and clindamycin were prescribed, and the patient remained hospitalized for 3 days. Seven days after surgical intervention, no maggots were observed. Our study emphasizes that dentists must recognize the symptoms and behaviors of parasitic diseases that affect the oral cavity.

  14. Oral Myiasis Caused by Cochliomyia hominivorax in a Disabled Person.

    Science.gov (United States)

    Novo-Neto, José Pereira; Dos Santos, Fabiano de Sant'Ana; Pontes, Ana Emília Farias; Ribeiro, Fernando Salimon; Scannavino, Fábio Luiz Ferreira; Martins, Alex Tadeu

    2015-01-01

    Myiasis is a parasitic disease caused by developing maggots of fly species, which can infect humans. Patients with special needs, especially those with severe neuropsychomotor limitations, may have oral manifestations of this disease. Here, we present a clinical case in which a disabled person was affected by oral myiasis caused by Cochliomyia hominivorax. Maggots were found in two ulcerated lesions, a 2 cm diameter lesion in the maxilla and a 6 cm diameter lesion in the mandible. Forty-five maggots were removed during inspection, whereas 75 maggots were surgically removed under general anesthesia with nasotracheal intubation. Dipyrone, ivermectin, and clindamycin were prescribed, and the patient remained hospitalized for 3 days. Seven days after surgical intervention, no maggots were observed. Our study emphasizes that dentists must recognize the symptoms and behaviors of parasitic diseases that affect the oral cavity.

  15. Data of cost-optimal solutions and retrofit design methods for school renovation in a warm climate.

    Science.gov (United States)

    Zacà, Ilaria; Tornese, Giuliano; Baglivo, Cristina; Congedo, Paolo Maria; D'Agostino, Delia

    2016-12-01

    "Efficient Solutions and Cost-Optimal Analysis for Existing School Buildings" (Paolo Maria Congedo, Delia D'Agostino, Cristina Baglivo, Giuliano Tornese, Ilaria Zacà) [1] is the paper that refers to this article. It reports the data related to the establishment of several variants of energy efficient retrofit measures selected for two existing school buildings located in the Mediterranean area. In compliance with the cost-optimal analysis described in the Energy Performance of Buildings Directive and its guidelines (EU, Directive, EU 244,) [2], [3], these data are useful for the integration of renewable energy sources and high performance technical systems for school renovation. The data of cost-efficient high performance solutions are provided in tables that are explained within the following sections. The data focus on the describe school refurbishment sector to which European policies and investments are directed. A methodological approach already used in previous studies about new buildings is followed (Baglivo Cristina, Congedo Paolo Maria, D׳Agostino Delia, Zacà Ilaria, 2015; IlariaZacà, Delia D'Agostino, Paolo Maria Congedo, Cristina Baglivo; Baglivo Cristina, Congedo Paolo Maria, D'Agostino Delia, Zacà Ilaria, 2015; Ilaria Zacà, Delia D'Agostino, Paolo Maria Congedo, Cristina Baglivo, 2015; Paolo Maria Congedo, Cristina Baglivo, IlariaZacà, Delia D'Agostino,2015) [4], [5], [6], [7], [8]. The files give the cost-optimal solutions for a kindergarten (REF1) and a nursery (REF2) school located in Sanarica and Squinzano (province of Lecce Southern Italy). The two reference buildings differ for construction period, materials and systems. The eleven tables provided contain data about the localization of the buildings, geometrical features and thermal properties of the envelope, as well as the energy efficiency measures related to walls, windows, heating, cooling, dhw and renewables. Output values of energy consumption, gas emission and costs are given for a

  16. Minor crops for export: a case study of boscalid, pyraclostrobin, lufenuron and lambda-cyhalothrin residue levels on green beans and spring onions in Egypt.

    Science.gov (United States)

    Hanafi, Ahmad; Garau, Vincenzo L; Caboni, Pierluigi; Sarais, Giorgia; Cabras, Paolo

    2010-08-01

    Dissipation rates of boscalid [2-chloro-N-(4' -chlorobiphenyl-2-yl)nicotinamide], pyraclostrobin [methyl 2-[1-(4-chlorophenyl) pyrazol-3-yloxymethyl]-N-methoxycarbanilate], lufenuron [(RS)-1-[2,5-dichloro-4-(1,1,2,3,3,3-hexafluoropropoxy)phenyl]-3-(2,6-difluorobenzoyl)urea] and lambda-cyhalothrin [(R)-cyano(3-phenoxyphenyl)methyl (1S,3S)-rel-3-[(1Z)-2-chloro-3,3,3-trifluoro-1-propenyl]-2,2-dimethylcyclopropanecarboxylate] in green beans and spring onions under Egyptian field conditions were studied. Field trials were carried out in 2008 in a Blue Nile farm, located at 70 kilometer (km) from Cairo (Egypt). The pesticides were sprayed at the recommended rate and samples were collected at pre-determined intervals. After treatment (T(0)) the pesticide residues in green beans were 7 times lower than in spring onions. This is due to a different structure of vegetable plant in the two crops. In spring onions, half-life (t(1/2)) of pyraclostrobin and lufenuron was 3.1 days and 9.8 days respectively. At day 14th (T(14)) after treatment boscalid residues were below the Maximum Residue Limit (MRL) (0.34 versus 0.5 mg/kg), pyraclostrobin and lambda -cyhalothrin residues were not detectable (ND), while lufenuron residues were above the MRL (0.06 versus 0.02 mg/kg). In green beans, at T(0), levels of boscalid, lufenuron and lambda -cyhalothrin were below the MRL (0.28 versus 2 mg/kg; ND versus 0.02 mg/kg; 0.06 versus 0.2 mg/kg, respectively) while, after 7 days treatment (T(7)) pyraclostrobin residues were above the MRL (0.03 versus 0.02 mg/kg). However, after 14 days the residue level could go below the MRL (0.02 mg/kg), as observed in spring onions.

  17. Potentiality of low cost environmentally friendly chamber for storage of irradiated potato and onion

    International Nuclear Information System (INIS)

    Roy, S.K.; Chakrabarti, A.K.; Pal, R.K.

    1994-01-01

    Potatoes and onions are two important horticultural commodities in India. Refrigeration is an expensive system and it is beyond the means of the poor, small and marginal farmers. Based on the principle of direct evaporative cooling, the scientists of the Indian Agricultural Research Institute developed a zero energy cool chamber. The zero energy cool chamber was tried at different locations in the country and was found to be very effective in increasing the shelf life of the fruits and vegetables and decreasing the physiological loss in weight

  18. Ultrasonic and dielectric studies of polymer PDMS composites with ZnO and onion-like carbons nanoinclusions

    OpenAIRE

    Samulionis, Vytautas; Macutkevič, Jan; Banys, Jūras; Shenderova, Olga

    2015-01-01

    The ultrasonic and dielectric temperature investigations were performed in polydi-methylsiloxane (PDMS) with zinc oxide (ZnO) and onion-like carbon (OLC) nanocomposites. In the glass transition region, the ultrasonic velocity dispersion and large ultrasonic attenuation maxima were observed. The positions of ultrasonic attenuation peaks were slightly shifted to higher temperatures after doping PDMS with OLC and ZnO nanoparticles. The ultrasonic relaxation was compared to that of dielectric and...

  19. Garlic, onions and cardiovascular risk factors. A review of the evidence from human experiments with emphasis on commercially available preparations

    NARCIS (Netherlands)

    Kleijnen, J.; Knipschild, P.; ter Riet, G.

    1989-01-01

    1. Claims for beneficial effects on cholesterol levels, fibrinolytic activity, and platelet aggregation are attributed both to fresh garlic and onions (or their extracts) and to commercially available preparations. 2. Regarding fresh garlic, the claims have been confirmed, but so far only at very

  20. Dynamics of Phenanthrenequinone on Carbon Nano-Onion Surfaces Probed by Quasielastic Neutron Scattering

    International Nuclear Information System (INIS)

    Mamontov, Eugene; Brown, Gilbert M.; Overbury, Steven H.; Mavila Chathoth, Suresh

    2012-01-01

    We used quasielastic neutron scattering (QENS) to study the dynamics of phenanthrenequinone (PQ) on the surface of onion-like carbon (OLC), or so called carbon onions, as a function of surface coverage and temperature. For both the high- and low-coverage samples, we observed two diffusion processes; a faster process and nearly an order of magnitude slower process. On the high-coverage surface, the slow diffusion process is of long-range translational character, whereas the fast diffusion process is spatially localized on the length scale of ∼ 4.7. On the low-coverage surface, both diffusion processes are spatially localized; on the same length scale of ∼ 4.7 for the fast diffusion and a somewhat larger length scale for the slow diffusion. Arrhenius temperature dependence is observed except for the long-range diffusion on the high-coverage surface. We attribute the fast diffusion process to the generic localized in-cage dynamics of PQ molecules, and the slow diffusion process to the long-range translational dynamics of PQ molecules, which, depending on the coverage, may be either spatially restricted, or long-range. On the low-coverage surface, uniform surface coverage is not attained, and the PQ molecules experience the effect of spatial constraints on their long-range translational dynamics. Unexpectedly, the dynamics of PQ molecules on OLC as a function of temperature and surface coverage bears qualitative resemblance to the dynamics of water molecules on oxide surfaces, including practically temperature-independent residence times for the low-coverage surface. The dynamics features that we observed may be universal across different classes of surface adsorbates.

  1. Effects of Onion (Allium cepa L. Extract Administration on Intestinal α-Glucosidases Activities and Spikes in Postprandial Blood Glucose Levels in SD Rats Model

    Directory of Open Access Journals (Sweden)

    Sun-Ho Kim

    2011-06-01

    Full Text Available Diets high in calories and sweetened foods with disaccharides frequently lead to exaggerated postprandial spikes in blood glucose. This state induces immediate oxidant stress and free radicals which trigger oxidative stress-linked diabetic complications. One of the therapeutic approaches for decreasing postprandial hyperglycemia is to retard absorption of glucose by the inhibition of carbohydrate hydrolyzing enzymes,α-amylase and α-glucosidases, in the digestive organs. Therefore, the inhibitory activity of Korean onion (Allium cepa L. extract against rat intestinal α-glucosidases, such as sucrase, maltase, and porcine pancreatic α-amylase were investigated in vitro and in vivo. The content of quercetin in ethyl alcohol extract of onion skin (EOS was 6.04 g/100 g dried weight of onion skin. The in vitro half-maximal inhibitory concentrations (IC50 of EOS and quercetin, a major phenolic in onion, on rat intestinal sucrase were 0.40 and 0.11 mg/mL, respectively. The postprandial blood glucose lowering effects of EOS and quercetin were compared to a known type 2 diabetes drug (Acarbose, a strong α-glucosidase inhibitor in the Sprague-Dawley (SD rat model. In rats fed on sucrose, EOS significantly reduced the blood glucose spike after sucrose loading. The area under the blood glucose-time curve (AUClast in EOS-treated SD rats (0.5 g-EOS/kg was significantly lower than in untreated SD rats (259.6 ± 5.1 vs. 283.1 ± 19.2 h·mg/dL. The AUClast in quercetin-treated SD rats (0.5 g-quercetin/kg was similar to in EOS-treated group (256.1 ± 3.2 vs. 259.6 ± 5.1 h·mg/dL. Results from this study indicates that although quercetin does have blood glucose lowering potential via α-glucosidase inhibition, there are other bioactive compounds present in onion skin. Furthermore, the effects of two weeks administration of EOS in a high carbohydrate-dietary mixture (Pico 5053 on sucrase and maltase activities in intestine were evaluated in SD rat model

  2. Comparison of carbon onions and carbon blacks as conductive additives for carbon supercapacitors in organic electrolytes

    Science.gov (United States)

    Jäckel, N.; Weingarth, D.; Zeiger, M.; Aslan, M.; Grobelsek, I.; Presser, V.

    2014-12-01

    This study investigates carbon onions (∼400 m2 g-1) as a conductive additive for supercapacitor electrodes of activated carbon and compares their performance with carbon black with high or low internal surface area. We provide a study of the electrical conductivity and electrochemical behavior between 2.5 and 20 mass% addition of each of these three additives to activated carbon. Structural characterization shows that the density of the resulting film electrodes depends on the degree of agglomeration and the amount of additive. Addition of low surface area carbon black (∼80 m2 g-1) enhances the power handling of carbon electrodes but significantly lowers the specific capacitance even when adding small amounts of carbon black. A much lower decrease in specific capacitance is observed for carbon onions and the best values are seen for carbon black with a high surface area (∼1390 m2 g-1). The overall performance benefits from the addition of any of the studied additives only at either high scan rates and/or electrolytes with high ion mobility. Normalization to the volume shows a severe decrease in volumetric capacitance and only at high current densities nearing 10 A g-1 we can see an improvement of the electrode capacitance.

  3. Antioxidative Activity of Onion Peel Extract in Obese Women: A Randomized, Double-blind, Placebo Controlled Study.

    Science.gov (United States)

    Kim, Kyung-Ah; Yim, Jung-Eun

    2015-09-01

    Quercetin, found abundantly in onion peel, has been known to have anticholesterol, antithrombotic and insulin-sensitizing properties. Here, we investigated the effect of quercetin-rich onion peel extract (OPE) on reactive oxygen species (ROS) production and antioxidative defense in obese woman. This study was randomized, double-blind, placebo controlled study. Thirty-seven healthy obese participants were randomly assigned that eighteen subjects received red soft capsuled OPE (100 mg/d, 50 mg bis in die), while the other nineteen subjects received same capsuled placebo for 12 weeks. ROS production and superoxide dismutase (SOD) activity in plasma were determined by using ROS and SOD assay kits, respectively. Baseline characteristics of anthropometric indicators and blood metabolic profiles were not significantly different between the two groups. Compared with baseline values, OPE consumption significantly reduced waist and hip circumference. Plasma ROS level and SOD activity were decreased in both placebo and OPE groups compared with baseline values. However, plasma ROS level in OPE group was significantly lower than in placebo group while plasma SOD activity in OPE group was significantly higher than in placebo group after 12 weeks of consumption. These findings indicate that OPE consumption may exert antioxidative effect by preventing the decrease of SOD activity as well as the production of ROS in obese women.

  4. The organic fertilization in the onion cultivation

    Directory of Open Access Journals (Sweden)

    Roberto Llorente Villa

    2016-02-01

    Full Text Available The present work was developed in the UBPC “The Coast”, belonging to the Miscellaneous Crops Enterprise of Campechuela municipality, Granma province, during the period from November to March of the year 2010 – 2011. It was carried out in a reddish brown fersialitic soil, according with the new genetic classification of the soils of Cuba MINAGRI, (2000, with the objective of studying and evaluating the influence of different organic fertilizers in the growth, development and yield in the cultivation of onion, variety Texas Early Grain. For this reason it was necessary to work on blocks with 4 treatments and 4 replies. The treatments applied were: treatment 1 (vermicompost, treatment 2 (cow dung, treatment 3 (cachaça and treatment 4 (control, those were applied in a solid way. The growth variables that were evaluated were number of leaves, height of the plant, equatorial diameter, polar diameter and fresh weight of the bulb. The yield was also evaluated in t.ha-1 per treatment. High yields are achieved with the application of organic fertilizer, among 27 and 30 t.ha-1, this is an alternative for the sustainable production of this product which is highly demanded by the population.

  5. Onion peel extracts ameliorate hyperglycemia and insulin resistance in high fat diet/streptozotocin-induced diabetic rats

    Directory of Open Access Journals (Sweden)

    Kim Ji Yeon

    2011-03-01

    Full Text Available Abstract Background Quercetin derivatives in onions have been regarded as the most important flavonoids to improve diabetic status in cells and animal models. The present study was aimed to examine the hypoglycemic and insulin-sensitizing capacity of onion peel extract (OPE containing high quercetin in high fat diet/streptozotocin-induced diabetic rats and to elucidate the mechanism of its insulin-sensitizing effect. Methods Male Sprague-Dawley rats were fed the AIN-93G diet modified to contain 41.2% fat and intraperitoneally injected with a single dose of streptozotocin (40 mg/kg body weight. One week after injection, the rats with fasting blood glucose levels above 126 mg/dL were randomly divided into 4 groups to treat with high fat diet containing 0 (diabetic control, 0.5, or 1% of OPE or 0.1% quercetin (quercetin equivalent to 1% of OPE for 8 weeks. To investigate the mechanism for the effects of OPE, we examined biochemical parameters (insulin sensitivity and oxidative stresses and protein and gene expressions (pro-inflammatory cytokines and receptors. Results Compared to the diabetic control, hypoglycemic and insulin-sensitizing capability of 1% OPE were demonstrated by significant improvement of glucose tolerance as expressed in incremental area under the curve (P = 0.0148. The insulin-sensitizing effect of OPE was further supported by increased glycogen levels in liver and skeletal muscle (P P = 0.0089, respectively. Quantitative RT-PCR analysis showed increased expression of insulin receptor (P = 0.0408 and GLUT4 (P = 0.0346 in muscle tissues. The oxidative stress, as assessed by superoxide dismutase activity and malondialdehyde formation, plasma free fatty acids, and hepatic protein expressions of IL-6 were significantly reduced by 1% OPE administration (P = 0.0393, 0.0237, 0.0148 and 0.0025, respectively. Conclusion OPE might improve glucose response and insulin resistance associated with type 2 diabetes by alleviating metabolic

  6. Sprout inhibition in garlic (Allium sativum) and onion (Allium cepa L.) by gamma irradiation. Part of a coordinated programme on pre-commercial scale radiation treatment of food

    International Nuclear Information System (INIS)

    Curzio, O.A.

    1982-04-01

    With the aim of verifying the possibilities and circumstances of sprout inhibition and storage life extension of onion and garlic by gamma irradiation, onion bulbs of variety Valenciana Sintetica 14 and garlic bulbs of a coloured locally grown variety were subjected to irradiation with 3 Krad of 60 Co gamma rays. The dose rate was 2440 rad/min; the irradiation conditions warranted a Dsub(max)/Dsub(min) ratio of 1.25. The irradiated bulbs and control samples of non-irradiated bulbs were investigated for a period of 270 to 330 days. Weight loss, external and internal sprouting, signs of decay, and the percentage of commercial bulbs were observed with the following results. Weight loss was found to be less in irradiated bulbs than in controls - 22% against 40% for onion and 33% against 65% for garlic. The dose of gamma radiation employed was proved to be sufficient for sprout inhibition in both species and for partial inhibition of decay and softening. The aroma of garlic was not impaired by irradiation. For both products, gamma irradiation was found to prolong the period of commercial utilizability

  7. Soft Robotic Manipulation of Onions and Artichokes in the Food Industry

    Directory of Open Access Journals (Sweden)

    R. Morales

    2014-04-01

    Full Text Available This paper presents the development of a robotic solution for a problem of fast manipulation and handling of onions or artichokes in the food industry. The complete solution consists of a parallel robotic manipulatior, a specially designed end-effector based on a customized vacuum suction cup, and a computer vision software developed for pick and place operations. First, the selection and design process of the proposed robotic solution to fit with the initial requeriments is presented, including the customized vacuum suction cup. Then, the kinematic analysis of the parallel manipulator needed to develop the robot control system is reviewed. Moreover, computer vision application is presented inthe paper. Hardware details of the implementation of the building prototype are also shown. Finally, conclusions and future work show the current status of the project.

  8. Indirect Effects of Field Management on Pollination Service and Seed Set in Hybrid Onion Seed Production.

    Science.gov (United States)

    Gillespie, Sandra; Long, Rachael; Williams, Neal

    2015-12-01

    Pollination in crops, as in native ecosystems, is a stepwise process that can be disrupted at any stage. Healthy pollinator populations are critical for adequate visitation, but pollination still might fail if crop management interferes with the attraction and retention of pollinators. Farmers must balance the direct benefits of applying insecticide and managing irrigation rates against their potential to indirectly interfere with the pollination process. We investigated these issues in hybrid onion seed production, where previous research has shown that high insecticide use reduces pollinator attraction. We conducted field surveys of soil moisture, nectar production, pollinator visitation, pollen-stigma interactions, and seed set at multiple commercial fields across 2 yr. We then examined how management actions, such as irrigation rate (approximated by soil moisture), or insecticide use could affect the pollination process. Onions produced maximum nectar at intermediate soil moisture, and high nectar production attracted more pollinators. Insecticide use weakly affected pollinator visitation, but when applied close to bloom reduced pollen germination and pollen tube growth. Ultimately, neither soil moisture nor insecticide use directly affected seed set, but the high correlation between pollinator visitation and seed set suggests that crop management will ultimately affect yields via indirect effects on the pollination process. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  9. Bio-fortification and isotopic labelling of Se metabolites in onions and carrots following foliar application of Se and 77Se

    DEFF Research Database (Denmark)

    Kápolna, Emese; Laursen, Kristian H.; Husted, Søren

    2012-01-01

    Se. The 77Se- labelled metabolites in onions were predominantly γ-glutamyl-77Se-selenomethyl-selenocysteine (γ-glu-Me77SeCys), 77Se-methylselenocysteine (Me77SeCys) and 77Se-selenomethionine (77SeMet). Furthermore, we report here for the first time the finding in carrots of the bioactive Me77SeCys...

  10. Characterization of onion genotypes by use of RAPD markers

    Directory of Open Access Journals (Sweden)

    Pavlović Nenad

    2012-01-01

    Full Text Available In order to estimate, at the molecular level, the divergence of parental lines that were used in diallel crossbreeding for production of superior offspring (F1 generation hybrids at the Institute for Vegetable Crops, the molecular analysis using five RAPD markers for five pairs of parents has been performed. It gives an insight into their genetic polymorphism and the possibility of their further use in breeding programs. Information from this research has pioneered the application of molecular markers of onion in Serbia. Analyses were performed using the RAPD primers, which in previous studies established a high degree of polymorphism. In all five cases there was a corresponding amplification of DNA segments. From totally 50 bands analyzed, the length of fragments ranged from 500 to 3000 bp. Number of polymorphic band per example was 8 to 13. In our research at the level of the analyzed primers, a high degree of polymorphism between analyzed genotypes has been found. Based on UPGMA dendogram, analyzed genotypes were divided into two main clusters and two subclusters.

  11. Studies on sprout inhibition of onions by gamma irradiation

    International Nuclear Information System (INIS)

    Thomas, P.; Srirangarajan, A.N.; Limaye, S.P.

    1975-01-01

    Sprout inhibition of onions by gamma irradiation was found to be influenced by the physiological state of the bulbs at the time of irradiation, radiation dose and the storage temperature after irradiation. Sprouting was minimal during storage at ambient temperature (26 to 32 0 C) while exposure to low temperature (4 to 20 0 C) or widely fluctuating diernal temperature (20 0 C min to 30 0 C max) accelerated it. Six to nine Krad inhibited sprouting during storage under the above conditions only if the bulbs were irradiated within a fortnight of harvest when they were in the dormant state. Twenty-five Krad caused a transient stimulation of sprouting regardless of the time of irradiation while 6 to 12 Krad only stimulated sprouting when the time interval between harvest and irradiation exceeded three weeks. Irradiation at all dose levels cause discoloration of the growth centre, the extent of which was minimal in bulbs exposed to low doses soon after harvest and in those stored at ambient temperature. (author)

  12. Verification of alternatives for sustainable onion production (Allium cepa L. in Cucaita, Boyaca

    Directory of Open Access Journals (Sweden)

    Viteri R. Silvio Edgar

    2012-04-01

    Full Text Available

    In Cucaita, onion production represents the basis of the economy and is concentrated in the valley but as yield declines due to deterioration of the soil, cultivation is shifting to the slopes, where unsustainability is more prominent. The aim of this study was to verify the potential of three alternatives for sustainable farming, using a split plot design on two farms. The alternatives were: 1 bocashi chicken manure, super broth four, fertilizer (BCmSB4F, 2 bocashi cow manure, rhizosphere broth, super broth four, fertilizer (BCmRBSB4F, 3 green manure, rhizosphere broth, super broth four, fertilizer (Gm- RBSB4F and 4 regional control (RC. This study determined disease incidence, plant height, stem and bulb diameter, healthy and diseased bulb weight and yield. The results indicated that regardless of the alternative, the agro-ecological conditions of the farm are critical to plant health and consequently to crop production. In El Arenal, where disease incidence was lower, the highest values for healthy bulb weight, bulb diameter and yield were revealed. The alternatives only differed in disease incidence at 30 and 45 days after transplant, with the lowest percentages corresponding to the green manure alternative. For revenue, the three alternatives were favorable, especially the BCmRBSB4F alternative which exceeded the RC. The results show that the alternatives GmRBSB4F and BCmRBSB4F have the most potential and are the most viable for sustainable onion production in Cucaita, Boyaca.

  13. Detonation nanodiamond and onion-like-carbon-embedded polyaniline for supercapacitors

    Energy Technology Data Exchange (ETDEWEB)

    Kovalenko, Igor; Bucknall, David G.; Yushin, Gleb [School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332 (United States)

    2010-11-23

    The detonation nanodiamond is a versatile low-cost nanomaterial with tunable properties and surface chemistry. In this work, it is shown how the application of nanodiamond (ND) can greatly increase the performance of electrochemically active polymers, such as polyaniline (PANI). Symmetric supercapacitors containing PANI-ND nanocomposite electrodes with 3-28 wt% ND show dramatically improved cycle stability and higher capacitance retention at fast sweep rate than pure PANI electrodes. Contrary to other PANI-carbon nanocomposites, specific capacitance of the selected PANI electrodes with embedded ND increases after 10 000 galvanostatic cycles and reaches 640 F g{sup -1}, when measured in a symmetric two-electrode configuration with 1 M H{sub 2}SO{sub 4} electrolyte. The demonstrated specific capacitance is 3-4 times higher than that of the activated carbons and more than 15 times higher than that of ND and onion-like carbon (OLC). (Copyright copyright 2010 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  14. Freezing Injury in Onion Bulb Cells

    Science.gov (United States)

    Palta, Jiwan P.; Levitt, Jacob; Stadelmann, Eduard J.

    1977-01-01

    Onion (Allium cepa L.) bulbs were frozen to −4 and −11 C and kept frozen for up to 12 days. After slow thawing, a 2.5-cm square from a bulb scale was transferred to 25 ml deionized H2O. After shaking for standard times, measurements were made on the effusate and on the effused cells. The results obtained were as follows. Even when the scale tissue was completely infiltrated, and when up to 85% of the ions had diffused out, all of the cells were still alive, as revealed by cytoplasmic streaming and ability to plasmolyze. The osmotic concentration of the cell sap, as measured plasmolytically, decreased in parallel to the rise in conductivity of the effusate. The K+ content of the effusate, plus its assumed counterion, accounted for only 20% of the total solutes, but for 100% of the conductivity. A large part of the nonelectrolytes in the remaining 80% of the solutes was sugars. The increased cell injury and infiltration in the −11 C treatment, relative to the −4 C and control (unfrozen) treatments, were paralleled by increases in conductivity, K+ content, sugar content, and pH of the effusate. In spite of the 100% infiltration of the tissue and the large increase in conductivity of the effusate following freezing, no increase in permeability of the cells to water could be detected. The above observations may indicate that freezing or thawing involves a disruption of the active transport system before the cells reveal any injury microscopically. PMID:16660100

  15. Transcriptome Analysis of Sucrose Metabolism during Bulb Swelling and Development in Onion (Allium cepa L.

    Directory of Open Access Journals (Sweden)

    Yi Liang

    2016-09-01

    Full Text Available Allium cepa L. is a widely cultivated and economically significant vegetable crop worldwide, with beneficial dietary and health-related properties, but its sucrose metabolism is still poorly understood. To analyze sucrose metabolism during bulb swelling, and the development of sweet taste in onion, a global transcriptome profile of onion bulbs was undertaken at three different developmental stages, using RNA-seq. A total of 79,376 unigenes, with a mean length of 678 bp, was obtained. In total, 7% of annotated Clusters of Orthologous Groups (COG were involved in carbohydrate transport and metabolism. In the Kyoto Encyclopedia of Genes and Genomes (KEGG database, starch and sucrose metabolism (147, 2.40% constituted the primary metabolism pathway in the integrated library. The expression of sucrose transporter genes was greatest during the early-swelling stage, suggesting that sucrose transporters participated in sucrose metabolism mainly at an early stage of bulb development. A gene-expression analysis of the key enzymes of sucrose metabolism suggested that sucrose synthase, cell wall invertase and invertase were all likely to participate in the hydrolysis of sucrose, generating glucose and fructose. In addition, trehalose was hydrolyzed to two molecules of glucose by trehalase. From 15 to 40 days after swelling (DAS, both the glucose and fructose contents of bulbs increased, whereas the sucrose content decreased. The growth rate between 15 and 30 DAS was slower than that between 30 and 40 DAS, suggesting that the latter was a period of rapid expansion. The dataset generated by our transcriptome profiling will provide valuable information for further research.

  16. Onion-shell model for cosmic ray electrons and radio synchrotron emission in supernova remnants

    International Nuclear Information System (INIS)

    Beck, R.; Drury, L.O.; Voelk, H.J.; Bogdan, T.J.

    1985-01-01

    The spectrum of cosmic ray electrons, accelerated in the shock front of a supernova remnant (SNR), is calculated in the test-particle approximation using an onion-shell model. Particle diffusion within the evolving remnant is explicity taken into account. The particle spectrum becomes steeper with increasing radius as well as SNR age. Simple models of the magnetic field distribution allow a prediction of the intensity and spectrum of radio synchrotron emission and their radial variation. The agreement with existing observations is satisfactory in several SNR's but fails in other cases. Radiative cooling may be an important effect, especially in SNR's exploding in a dense interstellar medium

  17. Onion-shell model for cosmic ray electrons and radio synchrotron emission in supernova remnants

    Science.gov (United States)

    Beck, R.; Drury, L. O.; Voelk, H. J.; Bogdan, T. J.

    1985-01-01

    The spectrum of cosmic ray electrons, accelerated in the shock front of a supernova remnant (SNR), is calculated in the test-particle approximation using an onion-shell model. Particle diffusion within the evolving remnant is explicity taken into account. The particle spectrum becomes steeper with increasing radius as well as SNR age. Simple models of the magnetic field distribution allow a prediction of the intensity and spectrum of radio synchrotron emission and their radial variation. The agreement with existing observations is satisfactory in several SNR's but fails in other cases. Radiative cooling may be an important effect, especially in SNR's exploding in a dense interstellar medium.

  18. Sulphur management in onion (Allium cepa) cultivation in hills of Himachal Pradesh.

    Science.gov (United States)

    Jaggi, R C; Sharma, R K

    2010-05-01

    Field experiment were conducted at CSK HPKV Research Farm, Palampur during Rabi seasons of 2000-01 and 2001-02, to study the response of onion (Allium cepa var Patna red) at four sulphur levels (0, 15, 30 and 60 kg ha(-1)) applied through Gypsum and S95. The analysis was done to allocate the limited availability of sulphur for maximizing net profit over fertilizer cost. The results show that the dose of sulphur under its full availability is 43.02 kg ha(-1). But under its scarce availability the maximum benefit would occur when it is applied up to 32.11 kg ha(-1) followed by even distribution of fertilizer i.e. 20 kg ha(-1). The returns following sulphur application at these rates, would be Rs 69340, 73092 and 68700 ha(-1) respectively.

  19. Enhanced Thermal Properties of Novel Latent Heat Thermal Storage Material Through Confinement of Stearic Acid in Meso-Structured Onion-Like Silica

    Science.gov (United States)

    Gao, Junkai; Lv, Mengjiao; Lu, Jinshu; Chen, Yan; Zhang, Zijun; Zhang, Xiongjie; Zhu, Yingying

    2017-12-01

    Meso-structured onion-like silica (MOS), which had a highly ordered, onion-like multilayer; large surface area and pore volume; and highly curved mesopores, were synthesized as a support for stearic acid (SA) to develop a novel shape-stabilized phase change material (SA/MOS). The characterizations of SA/MOS were studied by the analysis technique of scanning electron microscope, infrared spectroscopy, x-ray diffraction, differential scanning calorimeter (DSC), and thermal gravimetry analysis (TGA). The results showed that the interaction between the SA and the MOS was physical adsorption and that the MOS had no effect on the crystal structure of the SA. The DSC results suggested that the melting and solidifying temperature of the SA/MOS were 72.7°C and 63.9°C with a melting latent heat of 108.0 J/g and a solidifying latent heat of 126.0 J/g, respectively, and the TGA results indicated that the SA/MOS had a good thermal stability. All of the results demonstrated that the SA/MOS was a promising thermal energy storage material candidate for practical applications.

  20. Pesticide Residues in Commercial Lettuce, Onion, and Potato Samples From Bolivia—A Threat to Public Health?

    DEFF Research Database (Denmark)

    Skovgaard, Marlene; Renjel, Susana; Jensen, Olaf Chresten

    2017-01-01

    lettuce samples from La Paz were sampled on August 15, 2015 at a local market and screened for 283 pesticides. Residues of cypermethrin, chlorpyrifos, difenoconazol, or/and λ-cyhalothrin were detected in 50% of the lettuce samples, whereas no pesticides were found in potatoes and onions. In 20......% of the lettuce samples, the measurements were above the maximum residue limits, and 2 or 3 pesticides were identified simultaneously. Washing almost halved the pesticide levels, but still 20% of the samples showed measurements above the limits. No samples contained concentrations of pesticides which alone...