Quantitative detection of the potato cyst nematode, Globodera pallida, and the beet cyst nematode, Heterodera schachtii, using Real-Time PCR with SYBR green I dye.

Science.gov (United States)

Madani, Mehrdad; Subbotin, Sergei A; Moens, Maurice

2005-04-01

The potato cyst nematode Globodera pallida and the beet cyst nematode Heterodera schachtii are major nematode pests in world agriculture. Precise identification and knowledge about the number of nematodes in field soil are necessary to develop effective integrated pest control. Here we report the results of the Real-Time PCR assay for the rapid detection and quantification of G. pallida and H. schachtii. Using species specific primers and SYBR green I dye, we were able to detect a single second stage juvenile of cyst forming nematodes in samples. The specificity of the reaction was confirmed by the lack of amplification of DNAs from other Heterodera or Globodera species. Validation tests showed a rather high correlation between real numbers of second stage juveniles in a sample and expected numbers detected by Real-Time PCR. Reasons for observed differences in sensitivity and reliability of quantification detection for two species as well as other problems of Real-Time PCR are discussed. The Real-Time PCR assay with SYBR green I dye targeting fragments of the ITS-rDNA provided a sensitive means for the rapid and simultaneous detection and quantification of juveniles of these pests.

An Undergraduate Laboratory Experiment for Upper-Level Forensic Science, Biochemistry, or Molecular Biology Courses: Human DNA Amplification Using STR Single Locus Primers by Real-Time PCR with SYBR Green Detection

Science.gov (United States)

Elkins, Kelly M.; Kadunc, Raelynn E.

2012-01-01

In this laboratory experiment, real-time polymerase chain reaction (real-time PCR) was conducted using published human TPOX single-locus DNA primers for validation and various student-designed short tandem repeat (STR) primers for Combined DNA Index System (CODIS) loci. SYBR Green was used to detect the amplification of the expected amplicons. The…

Detection and characterization of Leishmania (Leishmania and Leishmania (Viannia by SYBR green-based real-time PCR and high resolution melt analysis targeting kinetoplast minicircle DNA.

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Marcello Ceccarelli

Full Text Available Leishmaniasis is a neglected disease with a broad clinical spectrum which includes asymptomatic infection. A thorough diagnosis, able to distinguish and quantify Leishmania parasites in a clinical sample, constitutes a key step in choosing an appropriate therapy, making an accurate prognosis and performing epidemiological studies. Several molecular techniques have been shown to be effective in the diagnosis of leishmaniasis. In particular, a number of PCR methods have been developed on various target DNA sequences including kinetoplast minicircle constant regions. The first aim of this study was to develop a SYBR green-based qPCR assay for Leishmania (Leishmania infantum detection and quantification, using kinetoplast minicircle constant region as target. To this end, two assays were compared: the first used previously published primer pairs (qPCR1, whereas the second used a nested primer pairs generating a shorter PCR product (qPCR2. The second aim of this study was to evaluate the possibility to discriminate among subgenera Leishmania (Leishmania and Leishmania (Viannia using the qPCR2 assay followed by melting or High Resolution Melt (HRM analysis. Both assays used in this study showed good sensitivity and specificity, and a good correlation with standard IFAT methods in 62 canine clinical samples. However, the qPCR2 assay allowed to discriminate between Leishmania (Leishmania and Leishmania (Viannia subgenera through melting or HRM analysis. In addition to developing assays, we investigated the number and genetic variability of kinetoplast minicircles in the Leishmania (L. infantum WHO international reference strain (MHOM/TN/80/IPT1, highlighting the presence of minicircle subclasses and sequence heterogeneity. Specifically, the kinetoplast minicircle number per cell was estimated to be 26,566±1,192, while the subclass of minicircles amplifiable by qPCR2 was estimated to be 1,263±115. This heterogeneity, also observed in canine clinical

Upconversion luminescence resonance energy transfer-based aptasensor for the sensitive detection of oxytetracycline.

Science.gov (United States)

Zhang, Hui; Fang, Congcong; Wu, Shijia; Duan, Nuo; Wang, Zhouping

2015-11-15

In this work, a biosensor based on luminescence resonance energy transfer (LRET) from NaYF4:Yb,Tm upconversion nanoparticles (UCNPs) to SYBR Green I has been developed. The aptamers are covalently linked to UCNPs and hybridized with their complementary strands. The subsequent addition of SYBR Green allows SYBR Green I to insert into the formed double-stranded DNA (dsDNA) duplex and brings the energy donor and acceptor into close proximity, leading to the fluorescence of UCNPs transferred to SYBR Green I. When excited at 980 nm, the UCNPs emit luminescence at 477 nm, and this energy is transferred to SYBR Green I, which emits luminescence at 530 nm. In the presence of oxytetracycline (OTC), the aptamers prefer to bind to its corresponding analyte and dehybridize with the complementary DNA. This dehybridization leads to the liberation of SYBR Green I, which distances SYBR Green I from the UCNPs and recovers the UCNPs' luminescence. Under optimal conditions, a linear calibration is obtained between the ratio of I530 to I477 nm (I530/I477) and the OTC concentration, which ranges from 0.1 to 10 ng/ml with a limit of detection (LOD) of 0.054 ng/ml. Copyright © 2015 Elsevier Inc. All rights reserved.

An Evaluation of Quantitative PCR Assays (TaqMan® and SYBR Green for the Detection of Babesia bigemina and Babesia bovis, and a Novel Fluorescent-ITS1-PCR Capillary Electrophoresis Method for Genotyping B. bovis Isolates

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Bing Zhang

2016-09-01

Full Text Available Babesia spp. are tick-transmitted haemoparasites causing tick fever in cattle. In Australia, economic losses to the cattle industry from tick fever are estimated at AUD$26 Million per annum. If animals recover from these infections, they become immune carriers. Here we describe a novel multiplex TaqMan qPCR targeting cytochrome b genes for the identification of Babesia spp. The assay shows high sensitivity, specificity and reproducibility, and allows quantification of parasite DNA from Babesia bovis and B. bigemina compared to standard PCR assays. A previously published cytochrome b SYBR Green qPCR was also tested in this study, showing slightly higher sensitivity than the Taqman qPCRs but requires melting curve analysis post-PCR to confirm specificity. The SYBR Green assays were further evaluated using both diagnostic submissions and vaccinated cattle (at 7, 9, 11 and 14 days post-inoculation showed that B. bigemina can be detected more frequently than B. bovis. Due to fewer circulating parasites, B. bovis detection in carrier animals requires higher DNA input. Preliminary data for a novel fluorescent PCR genotyping based on the Internal Transcribed Spacer 1 region to detect vaccine and field alleles of B. bovis are described. This assay is capable of detecting vaccine and novel field isolate alleles in a single sample.

A Multiplex SYBR Green Real-Time PCR Assay for the Detection of Three Colistin Resistance Genes from Cultured Bacteria, Feces, and Environment Samples

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Jiyun Li

2017-10-01

Full Text Available The aim of the study was to develop a multiplex assay for rapid detection of mcr-1, mcr-2, and mcr-3, a group of genes of conferring resistance to colistin mediated by plasmid in Enterobacteriaceae. A SYBR Green based real-time PCR assay has been designed to detect the mcr genes, and applied to cultured bacteria, feces and soil samples. All three mcr genes could be detected with a lower limit of 102 cultured bacteria. This test was highly specific and sensitive, and generated no false-positive results. The assay was also conclusive when applied to feces and soil samples containing mcr-1-positive Escherichia coli, which could facilitate the screening of mcr genes not only in the bacteria, but also directly from the environment. This simple, rapid, sensitive, and specific multiplex assay will be useful for rapid screening of the colistin resistance in both clinical medicine and animal husbandry.

One-step green synthesis of bimetallic Fe/Pd nanoparticles used to degrade Orange II

Energy Technology Data Exchange (ETDEWEB)

Luo, Fang; Yang, Die; Chen, Zuliang, E-mail: zuliang.chen@newcastle.edu.au; Megharaj, Mallavarapu; Naidu, Ravendra

2016-02-13

Highlights: • Green synthesis of bimetallic Fe/Pd NPs was firstly reported using the one-step method. • 98.0% of Orange II was removed by Fe/Pd NPs, but only 16.0% by Fe NPs. • Fe/Pd NPs with a diameter ranging from 10 to 100 nm were observed. • Removing Orange II using Fe/Pd NPs involved both adsorption and catalytic degradation. - Abstract: To reduce cost and enhance reactivity, bimetallic Fe/Pd nanoparticles (NPs) were firstly synthesized using grape leaf aqueous extract to remove Orange II. Green synthesized bimetallic Fe/Pd NPs (98.0%) demonstrated a far higher ability to remove Orange II in 12 h compared to Fe NPs (16.0%). Meanwhile, all precursors, e.g., grape leaf extract, Fe{sup 2+} and Pd{sup 2+}, had no obvious effect on removing Orange II since less than 2.0% was removed. Kinetics study revealed that the removal rate fitted well to the pseudo-first-order reduction and pseudo-second-order adsorption model, meaning that removing Orange II via Fe/Pd NPs involved both adsorption and catalytic reduction. The remarkable stability of Fe/Pd NPs showed the potential application for removing azo dyes. Furthermore, Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR) confirmed the changes in Fe/Pd NPs before and after reaction with Orange II. High Performance Liquid Chromatography–Mass Spectrum (HPLC–MS) identified the degraded products in the removal of Orange II, and finally a removal mechanism was proposed. This one-step strategy using grape leaf aqueous extract to synthesize Fe/Pd NPs is simple, cost-effective and environmentally benign, making possible the large-scale production of Fe/Pd NPs for field remediation.

Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions

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Puisieux Alain

2003-10-01

Full Text Available Abstract Background Real-time PCR is increasingly being adopted for RNA quantification and genetic analysis. At present the most popular real-time PCR assay is based on the hybridisation of a dual-labelled probe to the PCR product, and the development of a signal by loss of fluorescence quenching as PCR degrades the probe. Though this so-called 'TaqMan' approach has proved easy to optimise in practice, the dual-labelled probes are relatively expensive. Results We have designed a new assay based on SYBR-Green I binding that is quick, reliable, easily optimised and compares well with the published assay. Here we demonstrate its general applicability by measuring copy number in three different genetic contexts; the quantification of a gene rearrangement (T-cell receptor excision circles (TREC in peripheral blood mononuclear cells; the detection and quantification of GLI, MYC-C and MYC-N gene amplification in cell lines and cancer biopsies; and detection of deletions in the OPA1 gene in dominant optic atrophy. Conclusion Our assay has important clinical applications, providing accurate diagnostic results in less time, from less biopsy material and at less cost than assays currently employed such as FISH or Southern blotting.

Real-time PCR Detection of Brucella Abortus: A Comparative Study of SYBR Green I, 5'-exonuclease, and Hybridization Probe Assays

Energy Technology Data Exchange (ETDEWEB)

Newby, Deborah Trishelle; Hadfield, Ted; Roberto, Francisco Figueroa

2003-08-01

Real-time PCR provides a means of detecting and quantifying DNA targets by monitoring PCR product accumulation during cycling as indicated by increased fluorescence. A number of different approaches can be used to generate the fluorescence signal. Three approaches—SYBR Green I (a double-stranded DNA intercalating dye), 5'-exonuclease (enzymatically released fluors), and hybridization probes (fluorescence resonance energy transfer)—were evaluated for use in a real-time PCR assay to detect Brucella abortus. The three assays utilized the same amplification primers to produce an identical amplicon. This amplicon spans a region of the B. abortus genome that includes portions of the alkB gene and the IS711 insertion element. All three assays were of comparable sensitivity, providing a linear assay over 7 orders of magnitude (from 7.5 ng down to 7.5 fg). However, the greatest specificity was achieved with the hybridization probe assay.

Development of loop-mediated isothermal amplification and SYBR green real-time PCR methods for the detection of Citrus yellow mosaic badnavirus in citrus species.

Science.gov (United States)

Anthony Johnson, A M; Dasgupta, I; Sai Gopal, D V R

2014-07-01

Citrus yellow mosaic badnavirus (CMBV) is an important pathogen in southern India spread by infected citrus propagules. One of the measures to arrest the spread of CMBV is to develop methods to screen and certify citrus propagules as CMBV-free. The methods loop-mediated isothermal amplification (LAMP) and SYBR green real-time PCR (SGRTPCR) have been developed for the efficient detection of CMBV in citrus propagules. This paper compares the sensitivities of LAMP and SGRTPCR with polymerase chain reaction (PCR) for the detection of CMBV. Whereas PCR and LAMP were able to detect CMBV from a minimum of 10 ng of total DNA of infected leaf samples, SGRTPCR could detect the same from 1 ng of total DNA. Using SGRTPCR, the viral titres were estimated to be the highest in rough lemon and lowest in Nagpur Mandarin of the five naturally infected citrus species tested. The results will help in designing suitable strategies for the sensitive detection of CMBV from citrus propagules. Copyright © 2014 Elsevier B.V. All rights reserved.

Detection and Differentiation of Leishmania spp. in Clinical Specimens by Use of a SYBR Green-Based Real-Time PCR Assay.

Science.gov (United States)

de Almeida, Marcos E; Koru, Ozgur; Steurer, Francis; Herwaldt, Barbara L; da Silva, Alexandre J

2017-01-01

Leishmaniasis in humans is caused by Leishmania spp. in the subgenera Leishmania and Viannia Species identification often has clinical relevance. Until recently, our laboratory relied on conventional PCR amplification of the internal transcribed spacer 2 (ITS2) region (ITS2-PCR) followed by sequencing analysis of the PCR product to differentiate Leishmania spp. Here we describe a novel real-time quantitative PCR (qPCR) approach based on the SYBR green technology (LSG-qPCR), which uses genus-specific primers that target the ITS1 region and amplify DNA from at least 10 Leishmania spp., followed by analysis of the melting temperature (T m ) of the amplicons on qPCR platforms (the Mx3000P qPCR system [Stratagene-Agilent] and the 7500 real-time PCR system [ABI Life Technologies]). We initially evaluated the assay by testing reference Leishmania isolates and comparing the results with those from the conventional ITS2-PCR approach. Then we compared the results from the real-time and conventional molecular approaches for clinical specimens from 1,051 patients submitted to the reference laboratory of the Centers for Disease Control and Prevention for Leishmania diagnostic testing. Specimens from 477 patients tested positive for Leishmania spp. with the LSG-qPCR assay, specimens from 465 of these 477 patients also tested positive with the conventional ITS2-PCR approach, and specimens from 10 of these 465 patients had positive results because of retesting prompted by LSG-qPCR positivity. On the basis of the T m values of the LSG-qPCR amplicons from reference and clinical specimens, we were able to differentiate four groups of Leishmania parasites: the Viannia subgenus in aggregate; the Leishmania (Leishmania) donovani complex in aggregate; the species L (L) tropica; and the species L (L) mexicana, L (L) amazonensis, L (L) major, and L (L) aethiopica in aggregate. Copyright © 2016 American Society for Microbiology.

One-step, simple, and green synthesis of tin dioxide/graphene nanocomposites and their application to lithium-ion battery anodes

International Nuclear Information System (INIS)

Jiang, Zaixing; Zhang, Dongjie; Li, Yue; Cheng, Hao; Wang, Mingqiang; Wang, Xueqin; Bai, Yongping; Lv, Haibao; Yao, Yongtao; Shao, Lu; Huang, Yudong

2014-01-01

Highlights: • A one-step, simple and green approach to synthesis SnO 2 /graphene nanocomposites was proposed using a supercritical CO 2 method. • The SnO 2 /graphene nanocomposites was used as an anode, which exhibit extreme high lithium storage capacity and well cycling performance. - Abstract: Graphene with extraordinary thermal, mechanical and electrical properties offers possibilities in a variety of applications. Recent advances in the synthesis of graphene composites using supercritical fluids are highlighted. Supercritical fluids exhibit unique features for the synthesis of composites due to its low viscosity, high diffusivity, near-zero surface tension, and tunability. Here, we report the preparation of tin dioxide (SnO 2 )/graphene nanocomposite through supercritical CO 2 method. It demonstrates that the SnO 2 nanoparticles are homogeneously dispersed on the surface of graphene sheets with a particle size of 2.3–2.6 nm. The SnO 2 /graphene nanocomposites exhibit higher lithium storage capacity and better cycling performance compared to that of the similar CNT nanocomposites. The reported synthetic procedure is straightforward, green and inexpensive. And it may be readily adopted to produce large quantities of graphene based nanocomposites

The Healthy Steps Study: A randomized controlled trial of a pedometer-based Green Prescription for older adults. Trial protocol

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Schluter Philip J

2009-11-01

Full Text Available Abstract Background Graded health benefits of physical activity have been demonstrated for the reduction of coronary heart disease, some cancers, and type-2 diabetes, and for injury reduction and improvements in mental health. Older adults are particularly at risk of physical inactivity, and would greatly benefit from successful targeted physical activity interventions. Methods/Design The Healthy Steps study is a 12-month randomized controlled trial comparing the efficacy of a pedometer-based Green Prescription with the conventional time-based Green Prescription in increasing and maintaining physical activity levels in low-active adults over 65 years of age. The Green Prescription interventions involve a primary care physical activity prescription with 3 follow-up telephone counselling sessions delivered by trained physical activity counsellors over 3 months. Those in the pedometer group received a pedometer and counselling based around increasing steps that can be monitored on the pedometer, while those in the standard Green Prescription group received counselling using time-based goals. Baseline, 3 month (end of intervention, and 12 month measures were assessed in face-to-face home visits with outcomes measures being physical activity (Auckland Heart Study Physical Activity Questionnaire, quality of life (SF-36 and EQ-5D, depressive symptoms (Geriatric Depression Scale, blood pressure, weight status, functional status (gait speed, chair stands, and tandem balance test and falls and adverse events (self-report. Utilisation of health services was assessed for the economic evaluation carried out alongside this trial. As well, a process evaluation of the interventions and an examination of barriers and motives for physical activity in the sample were conducted. The perceptions of primary care physicians in relation to delivering physical activity counselling were also assessed. Discussion The findings from the Healthy Steps trial are due in late

Step-by-Step Simulation of Radiation Chemistry Using Green Functions for Diffusion-Influenced Reactions

Science.gov (United States)

Plante, Ianik; Cucinotta, Francis A.

2011-01-01

Radiolytic species are formed approximately 1 ps after the passage of ionizing radiation through matter. After their formation, they diffuse and chemically react with other radiolytic species and neighboring biological molecules, leading to various oxidative damage. Therefore, the simulation of radiation chemistry is of considerable importance to understand how radiolytic species damage biological molecules [1]. The step-by-step simulation of chemical reactions is difficult, because the radiolytic species are distributed non-homogeneously in the medium. Consequently, computational approaches based on Green functions for diffusion-influenced reactions should be used [2]. Recently, Green functions for more complex type of reactions have been published [3-4]. We have developed exact random variate generators of these Green functions [5], which will allow us to use them in radiation chemistry codes. Moreover, simulating chemistry using the Green functions is which is computationally very demanding, because the probabilities of reactions between each pair of particles should be evaluated at each timestep [2]. This kind of problem is well adapted for General Purpose Graphic Processing Units (GPGPU), which can handle a large number of similar calculations simultaneously. These new developments will allow us to include more complex reactions in chemistry codes, and to improve the calculation time. This code should be of importance to link radiation track structure simulations and DNA damage models.

Synthesis of carbon nanoparticles using one step green approach and their application as mercuric ion sensor

Energy Technology Data Exchange (ETDEWEB)

Roshni, V.; Ottoor, Divya, E-mail: divya@chem.unipune.ac.in

2015-05-15

Carbon nanoparticles (CNPs) have been evolved as a promising candidate for the metal sensing applications due to their synthesis from naturally occurring and easily available non-toxic molecular precursors by green chemistry. A simple and one step procedure is reported here for the synthesis of CNPs from coconut milk by thermal pyrolysis at a temperature of 120–150 °C for 2–5 min without using any carbonizing or passivating agent. On pyrolysis the coconut oil is separated from the carbon rich residue and the residue when dissolved in water showed blue fluorescence under UV light. The CNPs produced are found to show an emission maximum at 440 nm when excited at 360 nm. Synthesis by green approach makes CNPs a promising substitute for the metal sensing applications. Series of metal ions which have a hazardous impact on the ecological system have been taken for the analysis and it is observed that the fluorescence of CNPs gets remarkably quenched by mercuric ions. Fluorescence quenching was studied using standard Stern–Volmer quenching model. Limit of detection was found to be 16.5 nM Hg{sup 2+} concentration. - Highlights: • Green and economical synthesis of carbon nanoparticles (CNPs) from naturally abundant material. • Coconut milk is used as molecular precursor, which on thermal pyrolysis at 120 °C yielded CNPs. • Highly fluorescent CNPs show an emission maxima of 440 nm when excited at 360 nm. • Application of CNPs for metal ion sensing using fluorescence quenching phenomena. • Hg{sup 2+} is most effectively sensed with a detection limit of 16.5 nM.

Synthesis of carbon nanoparticles using one step green approach and their application as mercuric ion sensor

International Nuclear Information System (INIS)

Roshni, V.; Ottoor, Divya

2015-01-01

Carbon nanoparticles (CNPs) have been evolved as a promising candidate for the metal sensing applications due to their synthesis from naturally occurring and easily available non-toxic molecular precursors by green chemistry. A simple and one step procedure is reported here for the synthesis of CNPs from coconut milk by thermal pyrolysis at a temperature of 120–150 °C for 2–5 min without using any carbonizing or passivating agent. On pyrolysis the coconut oil is separated from the carbon rich residue and the residue when dissolved in water showed blue fluorescence under UV light. The CNPs produced are found to show an emission maximum at 440 nm when excited at 360 nm. Synthesis by green approach makes CNPs a promising substitute for the metal sensing applications. Series of metal ions which have a hazardous impact on the ecological system have been taken for the analysis and it is observed that the fluorescence of CNPs gets remarkably quenched by mercuric ions. Fluorescence quenching was studied using standard Stern–Volmer quenching model. Limit of detection was found to be 16.5 nM Hg 2+ concentration. - Highlights: • Green and economical synthesis of carbon nanoparticles (CNPs) from naturally abundant material. • Coconut milk is used as molecular precursor, which on thermal pyrolysis at 120 °C yielded CNPs. • Highly fluorescent CNPs show an emission maxima of 440 nm when excited at 360 nm. • Application of CNPs for metal ion sensing using fluorescence quenching phenomena. • Hg 2+ is most effectively sensed with a detection limit of 16.5 nM

One Step Quantum Key Distribution Based on EPR Entanglement.

Science.gov (United States)

Li, Jian; Li, Na; Li, Lei-Lei; Wang, Tao

2016-06-30

A novel quantum key distribution protocol is presented, based on entanglement and dense coding and allowing asymptotically secure key distribution. Considering the storage time limit of quantum bits, a grouping quantum key distribution protocol is proposed, which overcomes the vulnerability of first protocol and improves the maneuverability. Moreover, a security analysis is given and a simple type of eavesdropper's attack would introduce at least an error rate of 46.875%. Compared with the "Ping-pong" protocol involving two steps, the proposed protocol does not need to store the qubit and only involves one step.

Modulation of the Singlet Oxygen Generation from the Double Strand DNA-SYBR Green I Complex Mediated by T-Melamine-T Mismatch for Visual Detection of Melamine.

Science.gov (United States)

Hu, Hao; Zhang, Jinyi; Ding, Yu; Zhang, Xinfeng; Xu, Kailai; Hou, Xiandeng; Wu, Peng

2017-05-02

Singlet oxygen ( 1 O 2 ), generated via photosensitization, has been proved to oxidize chromogenic substrates with neither H 2 O 2 oxidation nor enzyme (horseradish peroxidase, HRP) catalysis. Of the various methods for modulation of the 1 O 2 generation, DNA-controlled photosensitization received great attention. Therefore, integration of the formation/deformation DNA structures with DNA-controlled photosensitization will be extremely appealing in visual biosensor developments. Here, the stable melamine-thymine complex was explored in combination with DNA-controlled photosensitization for visual detection of melamine. A T-rich single stand DNA was utilized as the recognition unit. Upon the formation of the T-M-T complex, double stand DNA was formed, which was ready for the binding of SYBR Green I and activated the photosensitization. Subsequent oxidation of TMB allowed visual detection of melamine in dairy products, with spike-recoveries ranging from 94% to 106%.

SU-F-J-66: Anatomy Deformation Based Comparison Between One-Step and Two-Step Optimization for Online ART

International Nuclear Information System (INIS)

Feng, Z; Yu, G; Qin, S; Li, D; Ma, C; Zhu, J; Yin, Y

2016-01-01

Purpose: This study investigated that how the quality of adapted plan was affected by inter-fractional anatomy deformation by using one-step and two-step optimization for on line adaptive radiotherapy (ART) procedure. Methods: 10 lung carcinoma patients were chosen randomly to produce IMRT plan by one-step and two-step algorithms respectively, and the prescribed dose was set as 60 Gy on the planning target volume (PTV) for all patients. To simulate inter-fractional target deformation, four specific cases were created by systematic anatomy variation; including target superior shift 0.5 cm, 0.3cm contraction, 0.3 cm expansion and 45-degree rotation. Based on these four anatomy deformation, adapted plan, regenerated plan and non-adapted plan were created to evaluate quality of adaptation. Adapted plans were generated automatically by using one-step and two-step algorithms respectively to optimize original plans, and regenerated plans were manually created by experience physicists. Non-adapted plans were produced by recalculating the dose distribution based on corresponding original plans. The deviations among these three plans were statistically analyzed by paired T-test. Results: In PTV superior shift case, adapted plans had significantly better PTV coverage by using two-step algorithm compared with one-step one, and meanwhile there was a significant difference of V95 by comparison with adapted and non-adapted plans (p=0.0025). In target contraction deformation, with almost same PTV coverage, the total lung received lower dose using one-step algorithm than two-step algorithm (p=0.0143,0.0126 for V20, Dmean respectively). In other two deformation cases, there were no significant differences observed by both two optimized algorithms. Conclusion: In geometry deformation such as target contraction, with comparable PTV coverage, one-step algorithm gave better OAR sparing than two-step algorithm. Reversely, the adaptation by using two-step algorithm had higher efficiency

SU-F-J-66: Anatomy Deformation Based Comparison Between One-Step and Two-Step Optimization for Online ART

Energy Technology Data Exchange (ETDEWEB)

Feng, Z; Yu, G; Qin, S; Li, D [Shandong Normal University, Jinan, Shandong (China); Ma, C; Zhu, J; Yin, Y [Shandong Cancer Hospital and Institute, Jinan, Shandong (China)

2016-06-15

Purpose: This study investigated that how the quality of adapted plan was affected by inter-fractional anatomy deformation by using one-step and two-step optimization for on line adaptive radiotherapy (ART) procedure. Methods: 10 lung carcinoma patients were chosen randomly to produce IMRT plan by one-step and two-step algorithms respectively, and the prescribed dose was set as 60 Gy on the planning target volume (PTV) for all patients. To simulate inter-fractional target deformation, four specific cases were created by systematic anatomy variation; including target superior shift 0.5 cm, 0.3cm contraction, 0.3 cm expansion and 45-degree rotation. Based on these four anatomy deformation, adapted plan, regenerated plan and non-adapted plan were created to evaluate quality of adaptation. Adapted plans were generated automatically by using one-step and two-step algorithms respectively to optimize original plans, and regenerated plans were manually created by experience physicists. Non-adapted plans were produced by recalculating the dose distribution based on corresponding original plans. The deviations among these three plans were statistically analyzed by paired T-test. Results: In PTV superior shift case, adapted plans had significantly better PTV coverage by using two-step algorithm compared with one-step one, and meanwhile there was a significant difference of V95 by comparison with adapted and non-adapted plans (p=0.0025). In target contraction deformation, with almost same PTV coverage, the total lung received lower dose using one-step algorithm than two-step algorithm (p=0.0143,0.0126 for V20, Dmean respectively). In other two deformation cases, there were no significant differences observed by both two optimized algorithms. Conclusion: In geometry deformation such as target contraction, with comparable PTV coverage, one-step algorithm gave better OAR sparing than two-step algorithm. Reversely, the adaptation by using two-step algorithm had higher efficiency

SYBR safeTM efficiently replaces ethidium bromide in Aspergillus fumigatus gene disruption.

Science.gov (United States)

Canela, H M S; Takami, L A; Ferreira, M E S

2017-02-08

Invasive aspergillosis is a disease responsible for high mortality rates, caused mainly by Aspergillus fumigatus. The available drugs are limited and this disease continues to occur at an unacceptable frequency. Gene disruption is essential in the search for new drug targets. An efficient protocol for A. fumigatus gene disruption was described but it requires ethidium bromide, a genotoxic agent, for DNA staining. Therefore, the present study tested SYBR safe TM , a non-genotoxic DNA stain, in A. fumigatus gene disruption protocol. The chosen gene was cipC, which has already been disrupted successfully in our laboratory. A deletion cassette was constructed in Saccharomyces cerevisiae and used in A. fumigatus transformation. There was no statistical difference between the tested DNA stains. The success rate of S. cerevisiae transformation was 63.3% for ethidium bromide and 70% for SYBR safe TM . For A. fumigatus gene disruption, the success rate for ethidium bromide was 100 and 97% for SYBR safe TM . In conclusion, SYBR safe TM efficiently replaced ethidium bromide, making this dye a safe and efficient alternative for DNA staining in A. fumigatus gene disruption.

Further step beyond green - From distractive, to balance, towards restorative built environment

Directory of Open Access Journals (Sweden)

AbdelRahman AbdelNaeem AbdelLatif Mohamed

2017-12-01

Many new movements, like biophilic design, have recently gained much momentum within the building community, which provide further step beyond green to restore beneficial contacts between people and natural environment. While the green movement has often focused on the means, biophilic design tends toward emphasizing the end results, establishing natural-based habitats for humans to live and work. Biophilic Design incorporates green building ideas, but considers that true sustainability must include quality of life issues involving human physiological & psychological-health and well-being. So, Biophilic design incorporates elements derived from nature in order to maximize human functioning and health.

SYBR green-based real-time reverse transcription-PCR for typing and subtyping of all hemagglutinin and neuraminidase genes of avian influenza viruses and comparison to standard serological subtyping tests

Science.gov (United States)

Tsukamoto, K.; Javier, P.C.; Shishido, M.; Noguchi, D.; Pearce, J.; Kang, H.-M.; Jeong, O.M.; Lee, Y.-J.; Nakanishi, K.; Ashizawa, T.

2012-01-01

Continuing outbreaks of H5N1 highly pathogenic (HP) avian influenza virus (AIV) infections of wild birds and poultry worldwide emphasize the need for global surveillance of wild birds. To support the future surveillance activities, we developed a SYBR green-based, real-time reverse transcriptase PCR (rRT-PCR) for detecting nucleoprotein (NP) genes and subtyping 16 hemagglutinin (HA) and 9 neuraminidase (NA) genes simultaneously. Primers were improved by focusing on Eurasian or North American lineage genes; the number of mixed-base positions per primer was set to five or fewer, and the concentration of each primer set was optimized empirically. Also, 30 cycles of amplification of 1:10 dilutions of cDNAs from cultured viruses effectively reduced minor cross- or nonspecific reactions. Under these conditions, 346 HA and 345 NA genes of 349 AIVs were detected, with average sensitivities of NP, HA, and NA genes of 10 1.5, 10 2.3, and 10 3.1 50% egg infective doses, respectively. Utility of rRT-PCR for subtyping AIVs was compared with that of current standard serological tests by using 104 recent migratory duck virus isolates. As a result, all HA genes and 99% of the NA genes were genetically subtyped, while only 45% of HA genes and 74% of NA genes were serologically subtyped. Additionally, direct subtyping of AIVs in fecal samples was possible by 40 cycles of amplification: approximately 70% of HA and NA genes of NP gene-positive samples were successfully subtyped. This validation study indicates that rRT-PCR with optimized primers and reaction conditions is a powerful tool for subtyping varied AIVs in clinical and cultured samples. Copyright ?? 2012, American Society for Microbiology. All Rights Reserved.

Touch-down reverse transcriptase-PCR detection of IgV(H) rearrangement and Sybr-Green-based real-time RT-PCR quantitation of minimal residual disease in patients with chronic lymphocytic leukemia.

Science.gov (United States)

Peková, Sona; Marková, Jana; Pajer, Petr; Dvorák, Michal; Cetkovský, Petr; Schwarz, Jirí

2005-01-01

Patients with chronic lymphocytic leukemia (CLL) can relapse even after aggressive therapy and autografts. It is commonly assumed that to prevent relapse the level of minimal residual disease (MRD) should be as low as possible. To evaluate MRD, highly sensitive quantitative assays are needed. The aim of the study was to develop a robust and sensitive method for detection of the clonal immunoglobulin heavy-chain variable (IgV(H)) rearrangement in CLL and to introduce a highly sensitive and specific methodology for MRD monitoring in patients with CLL who undergo intensive treatment. As a prerequisite for MRD detection, touch-down reverse transcriptase (RT)-PCR using degenerate primers were used for the diagnostic identification of (H) gene rearrangement(s). For quantitative MRD detection in 18 patients, we employed a real-time RT-PCR assay (RQ-PCR) making use of patient-specific primers and the cost-saving Sybr-Green reporter dye (SG). For precise calibration of RQ-PCR, patient-specific IgV(H) sequences were cloned. Touch-down RT-PCR with degenerate primers allowed the successful detection of IgV(H) clonal rearrangement(s) in 252 of 257 (98.1%) diagnostic samples. Biallelic rearrangements were found in 27 of 252 (10.7%) cases. Degenerate primers used for the identification of clonal expansion at diagnosis were not sensitive enough for MRD detection. In contrast, our RQ-PCR assay using patient-specific primers and SG reached the sensitivity of 10(-)(6). We demonstrated MRD in each patient tested, including four of four patients in complete remission following autologous hematopoietic stem cell transplantation (HSCT) and three of three following allogeneic 'mini'-HSCT. Increments in MRD might herald relapse; aggressive chemotherapy could induce molecular remission. Our touch-down RT-PCR has higher efficiency to detect clonal IgV(H) rearrangements including the biallelic ones. MRD quantitation of IgV(H) expression using SG-based RQ-PCR represents a highly specific

Cross-platform comparison of SYBR® Green real-time PCR with TaqMan PCR, microarrays and other gene expression measurement technologies evaluated in the MicroArray Quality Control (MAQC study

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Dial Stacey L

2008-07-01

Full Text Available Abstract Background The MicroArray Quality Control (MAQC project evaluated the inter- and intra-platform reproducibility of seven microarray platforms and three quantitative gene expression assays in profiling the expression of two commercially available Reference RNA samples (Nat Biotechnol 24:1115-22, 2006. The tested microarrays were the platforms from Affymetrix, Agilent Technologies, Applied Biosystems, GE Healthcare, Illumina, Eppendorf and the National Cancer Institute, and quantitative gene expression assays included TaqMan® Gene Expression PCR Assay, Standardized (Sta RT-PCR™ and QuantiGene®. The data showed great consistency in gene expression measurements across different microarray platforms, different technologies and test sites. However, SYBR® Green real-time PCR, another common technique utilized by half of all real-time PCR users for gene expression measurement, was not addressed in the MAQC study. In the present study, we compared the performance of SYBR Green PCR with TaqMan PCR, microarrays and other quantitative technologies using the same two Reference RNA samples as the MAQC project. We assessed SYBR Green real-time PCR using commercially available RT2 Profiler™ PCR Arrays from SuperArray, containing primer pairs that have been experimentally validated to ensure gene-specificity and high amplification efficiency. Results The SYBR Green PCR Arrays exhibit good reproducibility among different users, PCR instruments and test sites. In addition, the SYBR Green PCR Arrays have the highest concordance with TaqMan PCR, and a high level of concordance with other quantitative methods and microarrays that were evaluated in this study in terms of fold-change correlation and overlap of lists of differentially expressed genes. Conclusion These data demonstrate that SYBR Green real-time PCR delivers highly comparable results in gene expression measurement with TaqMan PCR and other high-density microarrays.

Fluorometric determination of nucleic acids based on the use of polydopamine nanotubes and target-induced strand displacement amplification.

Science.gov (United States)

Ge, Jia; Bai, Dong-Mei; -Geng, Xin; Hu, Ya-Lei; Cai, Qi-Yong; Xing, Ke; Zhang, Lin; Li, Zhao-Hui

2018-01-10

The authors describe a fluorometric method for the quantitation of nucleic acids by combining (a) cycled strand displacement amplification, (b) the unique features of the DNA probe SYBR Green, and (c) polydopamine nanotubes. SYBR Green undergoes strong fluorescence enhancement upon intercalation into double-stranded DNA (dsDNA). The polydopamine nanotubes selectively adsorb single-stranded DNA (ssDNA) and molecular beacons. In the absence of target DNA, the molecular beacon, primer and SYBR Green are adsorbed on the surface of polydopamine nanotubes. This results in quenching of the fluorescence of SYBR Green, typically measured at excitation/emission wavelengths of 488/518 nm. Upon addition of analyte (target DNA) and polymerase, the stem of the molecular beacon is opened so that it can bind to the primer. This triggers target strand displacement polymerization, during which dsDNA is synthesized. The hybridized target is then displaced due to the strand displacement activity of the polymerase. The displaced target hybridizes with another molecular beacon. This triggers the next round of polymerization. Consequently, a large amount of dsDNA is formed which is detected by addition of SYBR Green. Thus, sensitive and selective fluorometric detection is realized. The fluorescent sensing strategy shows very good analytical performances towards DNA detection, such as a wide linear range from 0.05 to 25 nM with a low limit of detection of 20 pM. Graphical abstract Schematic of a fluorometric strategy for highly sensitive and selective determination of nucleic acids by combining strand displacement amplification and the unique features of SYBR Green I (SG) and polydopamine nanotubes.

One-step green route to narrowly dispersed copper nanocrystals

Energy Technology Data Exchange (ETDEWEB)

Wu Chunwei; Mosher, Brian P.; Zeng Taofang [North Carolina State University, Mechanical and Aerospace Engineering (United States)], E-mail: tzeng2@unity.ncsu.edu

2006-12-15

We report a total 'green' chemical method in aqueous solution for synthesizing stable narrowly distributed copper nanoparticles with average diameter less than 5 nm in the presence of Polyvinylpyrrolidone (PVP) as a stabilizer and without any inert gas protection. In our synthesis route, ascorbic acid, natural vitamin C (VC), an excellent oxygen scavenger, acts as both reducing agent and antioxidant, to reduce the metallic ion precursor, and to effectively prevent the common oxidation process of the newborn pure copper nanoclusters.

Quantification of ochratoxin A-producing molds in food products by SYBR Green and TaqMan real-time PCR methods

DEFF Research Database (Denmark)

Rodríguez, Alicia; Rodríguez, Mar; Luque, M. Isabel

2011-01-01

, usually reported in food products, were used as references. All strains were tested for OTA production by mycellar electrokinetic capillary electrophoresis (MECE) and high-pressure liquid chromatography-mass spectrometry (HPLC-MS). The ability of the optimized qPCR protocols to quantify OTA......Ochratoxin A (OTA) is a mycotoxin synthesized by a variety of different fungi, most of them from the genera Penicillium and Aspergillus. Early detection and quantification of OTA producing species is crucial to improve food safety. In the present work, two protocols of real-time qPCR based on SYBR......-producing molds was evaluated in different artificially inoculated foods. A good linear correlation was obtained over the range 1 x 104 to 10 conidia/g per reaction for all qPCR assays in the different food matrices (cooked and cured products and fruits). The detection limit in all inoculated foods ranged between...

One-step green synthesis of non-hazardous dicarboxyl cellulose flocculant and its flocculation activity evaluation

International Nuclear Information System (INIS)

Zhu, Hangcheng; Zhang, Yong; Yang, Xiaogang; Liu, Hongyi; Shao, Lan; Zhang, Xiumei; Yao, Juming

2015-01-01

The waste management of used flocculants is a thorny issue in the field of wastewater treatment. To natural cellulose based flocculants, utilization of hazardous cellulose solvent and simplification of synthetic procedure are the two urgent problems needing to be further improved. In this work, a series of natural dicarboxyl cellulose flocculants (DCCs) were one-step synthesized via Schiff-base route. The cellulose solvent (NaOH/Urea solution) was utilized during the synthesis process. The full-biodegradable flocculants avoid causing secondary pollution to environment. The chemical structure and solution property of the DCC products were characterized by FT-IR, 1 H NMR, 13 C NMR, TGA, FESEM, charge density and ζ-potential. Kaolin suspension and effluent from paper mill were selected to evaluate the flocculation activity of the DCCs. Their flocculation performance was compared with that of commercial cationic polyacrylamide and poly aluminium chloride flocculants. The positive results showed that the NaOH/Urea solvent effectively promoted the dialdehyde cellulose (DAC) conversion to DCC in the one-step synthesis reaction. The DCCs with the carboxylate content more than 1 mmol/g exhibited steady flocculation performance to kaolin suspension in the broad pH range from 4 to 10. Its flocculation capacity to the effluent from paper mill also showed excellent

Identification of Listeria monocytogenes on Green Mussels and Cockle Shell

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Winiati Puji Rahayu

2017-02-01

Full Text Available AbstractGreen mussel (Perna viridis and cockle shell (Anadara granosa are one of many sources of animal protein which is many cultivated in Indonesia because their price is relatively affordable. This study was conducted to identify the presence of Listeria monocytogenes in 27 samples of green mussels and 3 samples of cockle shells using real-time Polymerase Chain Reaction (real-time PCR and biochemical methods. The target gene for amplification in real-time PCR was an hlyA gene because this gene was a determinant of virulence genes that produce listeriolysin O. Primers used in this study were forward primer DG69 (GTG CCG GGT AAA AGA CCA TA and reverse primer DG74 (CGC CAC TGA GAT ACT AT and fluorescence signals indicator using SYBR Green I. The results of analysis using real-time PCR were negative Listeria monocytogenes in all samples, while using biochemical methods there was one of 30 samples contaminated by Listeria welshimeri.

One-step fabrication of multifunctional micromotors

Science.gov (United States)

Gao, Wenlong; Liu, Mei; Liu, Limei; Zhang, Hui; Dong, Bin; Li, Christopher Y.

2015-08-01

Although artificial micromotors have undergone tremendous progress in recent years, their fabrication normally requires complex steps or expensive equipment. In this paper, we report a facile one-step method based on an emulsion solvent evaporation process to fabricate multifunctional micromotors. By simultaneously incorporating various components into an oil-in-water droplet, upon emulsification and solidification, a sphere-shaped, asymmetric, and multifunctional micromotor is formed. Some of the attractive functions of this model micromotor include autonomous movement in high ionic strength solution, remote control, enzymatic disassembly and sustained release. This one-step, versatile fabrication method can be easily scaled up and therefore may have great potential in mass production of multifunctional micromotors for a wide range of practical applications.Although artificial micromotors have undergone tremendous progress in recent years, their fabrication normally requires complex steps or expensive equipment. In this paper, we report a facile one-step method based on an emulsion solvent evaporation process to fabricate multifunctional micromotors. By simultaneously incorporating various components into an oil-in-water droplet, upon emulsification and solidification, a sphere-shaped, asymmetric, and multifunctional micromotor is formed. Some of the attractive functions of this model micromotor include autonomous movement in high ionic strength solution, remote control, enzymatic disassembly and sustained release. This one-step, versatile fabrication method can be easily scaled up and therefore may have great potential in mass production of multifunctional micromotors for a wide range of practical applications. Electronic supplementary information (ESI) available: Videos S1-S4 and Fig. S1-S3. See DOI: 10.1039/c5nr03574k

Identification of Listeria monocytogenes on Green Mussels (Perna viridis and Cockle Shell (Anadara granosa

Directory of Open Access Journals (Sweden)

Winiati Puji Rahayu

2016-12-01

Full Text Available Green mussel (Perna viridis and cockle shell (Anadara granosa are one of many sources of animalprotein which is many cultivated in Indonesia because their price is relatively affordable. This study wasconducted to identify the presence of Listeria monocytogenes in 27 samples of green mussels and 3 samplesof cockle shells using real-time Polymerase Chain Reaction (real-time PCR and biochemical methods. Thetarget gene for amplification in real-time PCR was an hlyA gene because this gene was a determinant ofvirulence genes that produce listeriolysin O. Primers used in this study were forward primer DG69 (GTGCCG GGT AAA AGA CCA TA and reverse primer DG74 (CGC CAC TGA GAT ACT AT and fluorescencesignals indicator using SYBR Green I. The results of analysis using real-time PCR were negative Listeriamonocytogenes in all samples, while using biochemical methods there was one of 30 samples contaminatedby Listeria welshimeri.

One-step in situ synthesis of SnO2/graphene nanocomposites and its application as an anode material for Li-ion batteries.

Science.gov (United States)

Liang, Junfei; Wei, Wei; Zhong, Da; Yang, Qinglin; Li, Lidong; Guo, Lin

2012-01-01

A facile one-step solution-based process to in situ synthesize SnO(2)/graphene (SG) nanocomposites was developed, by using the mixture of dimethyl sulfoxide (DMSO) and H(2)O as both solvent and reactant. The reduction of graphene oxide (GO) and the in situ formation of SnO(2) nanoparticles were realized in one step. The electrochemical experiments showed the composites provided a better Li-storage performance. The method presented in this paper may provide an effective, economic, and green strategy for the preparation of metal-oxide/graphene nanocomposites. © 2011 American Chemical Society

One-step synthesis of lightly doped porous silicon nanowires in HF/AgNO3/H2O2 solution at room temperature

International Nuclear Information System (INIS)

Bai, Fan; Li, Meicheng; Song, Dandan; Yu, Hang; Jiang, Bing; Li, Yingfeng

2012-01-01

One-step synthesis of lightly doped porous silicon nanowire arrays was achieved by etching the silicon wafer in HF/AgNO 3 /H 2 O 2 solution at room temperature. The lightly doped porous silicon nanowires (pNWs) have circular nanopores on the sidewall, which can emit strong green fluorescence. The surface morphologies of these nanowires could be controlled by simply adjusting the concentration of H 2 O 2 , which influences the distribution of silver nanoparticles (Ag NPs) along the nanowire axis. A mechanism based on Ag NPs-induced lateral etching of nanowires was proposed to explain the formation of pNWs. The controllable and widely applicable synthesis of pNWs will open their potential application to nanoscale photoluminescence devices. - Graphical abstract: The one-step synthesis of porous silicon nanowire arrays is achieved by chemical etching of the lightly doped p-type Si (100) wafer at room temperature. These nanowires exhibit strong green photoluminescence. SEM, TEM, HRTEM and photoluminescence images of pNWs. The scale bars of SEM, TEM HRTEM and photoluminescence are 10 μm, 20 nm, 10 nm, and 1 μm, respectively. Highlights: ► Simple one-step synthesis of lightly doped porous silicon nanowire arrays is achieved at RT. ► Etching process and mechanism are illustrated with etching model from a novel standpoint. ► As-prepared porous silicon nanowire emits strong green fluorescence, proving unique property.

One-step solvothermal synthesis, a worm-shaped morphology and luminescence properties of green-emitting Y2O2S:Tb3+ nanophosphors

Science.gov (United States)

Liu, Fan; Lian, Jingbao; Wu, Nianchu; He, Jiao; Zhang, Xue; Liu, Feng

2018-02-01

The worm-shaped Y2O2S:Tb3+ nanophosphors have been successfully prepared via one-step solvothermal synthesis, using ethanediamine as the main solvent and sublimed sulfur powder as the source of sulfur without adding additional structure-directing reagents. XRD, FT-IR, FESEM, PL and CIE chromaticity diagram were used to characterize the obtained products. XRD results demonstrate that all diffraction peaks of the sample can be well indexed to pure hexagonal phase of Y2O2S with optimal condition, i.e. the molar ration of S/Y3+m = 10, solvothermal temperature T = 220 °C and synthetic time t = 24 h. When t varies from 2 h to 24 h, the morphology of Y2O2S transforms from cauliflower-like structure to worm-shaped nanoparticles with the length of ∼80 nm. The formation mechanism depending on t has also been proposed. Upon 250 nm ultraviolet (UV) light excitation, the worm-shaped Y2O2S:Tb3+ nanophosphors exhibit green emissions, corresponding to the 5D4 → 7FJ (J = 6, 5, 4, 3) transitions of Tb3+ions. The quenching concentration is 7% and its corresponding lifetime is 1216 μs. The CIE chromaticity coordinates show the tuneable emission shifting from yellow green to yellowish green with increasing concentration of Tb3+ions from 1% to 7%. Those results suggest that the worm-shaped Y2O2S:Tb3+ nanophosphors may have potential applications in X-ray intensifying screens, fluorescence and biomedical fields.

Fuzzy Axiomatic Design approach based green supplier selection

DEFF Research Database (Denmark)

Kannan, Devika; Govindan, Kannan; Rajendran, Sivakumar

2015-01-01

proposes a multi-criteria decision-making (MCDM) approach called Fuzzy Axiomatic Design (FAD) to select the best green supplier for Singapore-based plastic manufacturing company. At first, the environmental criteria was developed along with the traditional criteria based on the literature review......Abstract Green Supply Chain Management (GSCM) is a developing concept recently utilized by manufacturing firms of all sizes. All industries, small or large, seek improvements in the purchasing of raw materials, manufacturing, allocation, transportation efficiency, in curbing storage time, importing...... responsible in addition to being efficiently managed. A significant way to implement responsible GSCM is to reconsider, in innovative ways, the purchase and supply cycle, and a preliminary step would be to ensure that the supplier of goods successfully incorporates green criteria. Therefore, this paper...

Thread-like supercapacitors based on one-step spun nanocomposite yarns.

Science.gov (United States)

Meng, Qinghai; Wang, Kai; Guo, Wei; Fang, Jin; Wei, Zhixiang; She, Xilin

2014-08-13

Thread-like electronic devices have attracted great interest because of their potential applications in wearable electronics. To produce high-performance, thread-like supercapacitors, a mixture of stable dispersions of single-walled carbon nanotubes and conducting polyaniline nanowires are prepared. Then, the mixture is spun into flexible yarns with a polyvinyl alcohol outer sheath by a one-step spinning process. The composite yarns show excellent mechanical properties and high electrical conductivities after sufficient washing to remove surfactants. After applying a further coating layer of gel electrolyte, two flexible yarns are twisted together to form a thread-like supercapacitor. The supercapacitor based on these two yarns (SWCNTs and PAniNWs) possesses a much higher specific capacitance than that based only on pure SWCNTs yarns, making it an ideal energy-storage device for wearable electronics. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Green ICT in 8 steps; Groene ICT in 8 stappen

Energy Technology Data Exchange (ETDEWEB)

Bijlsma, J.

2011-02-15

ICT related energy use is an important cost factor. This white paper offers a plan for green ICT in 8 steps. [Dutch] ICT-gerelateerd energiegebruik is een belangrijke kostenfactor. In deze 'white paper' wordt in 8 stappen een plan voor groene ICT uit de doeken gedaan.

Enhanced capillary electrophoretic screening of Alzheimer based on direct apolipoprotein E genotyping and one-step multiplex PCR.

Science.gov (United States)

Woo, Nain; Kim, Su-Kang; Sun, Yucheng; Kang, Seong Ho

2018-01-01

Human apolipoprotein E (ApoE) is associated with high cholesterol levels, coronary artery disease, and especially Alzheimer's disease. In this study, we developed an ApoE genotyping and one-step multiplex polymerase chain reaction (PCR) based-capillary electrophoresis (CE) method for the enhanced diagnosis of Alzheimer's. The primer mixture of ApoE genes enabled the performance of direct one-step multiplex PCR from whole blood without DNA purification. The combination of direct ApoE genotyping and one-step multiplex PCR minimized the risk of DNA loss or contamination due to the process of DNA purification. All amplified PCR products with different DNA lengths (112-, 253-, 308-, 444-, and 514-bp DNA) of the ApoE genes were analyzed within 2min by an extended voltage programming (VP)-based CE under the optimal conditions. The extended VP-based CE method was at least 120-180 times faster than conventional slab gel electrophoresis methods In particular, all amplified DNA fragments were detected in less than 10 PCR cycles using a laser-induced fluorescence detector. The detection limits of the ApoE genes were 6.4-62.0pM, which were approximately 100-100,000 times more sensitive than previous Alzheimer's diagnosis methods In addition, the combined one-step multiplex PCR and extended VP-based CE method was also successfully applied to the analysis of ApoE genotypes in Alzheimer's patients and normal samples and confirmed the distribution probability of allele frequencies. This combination of direct one-step multiplex PCR and an extended VP-based CE method should increase the diagnostic reliability of Alzheimer's with high sensitivity and short analysis time even with direct use of whole blood. Copyright © 2017 Elsevier B.V. All rights reserved.

Exogenous ROS-induced cell sheet transfer based on hematoporphyrin-polyketone film via a one-step process.

Science.gov (United States)

Koo, Min-Ah; Lee, Mi Hee; Kwon, Byeong-Ju; Seon, Gyeung Mi; Kim, Min Sung; Kim, Dohyun; Nam, Ki Chang; Park, Jong-Chul

2018-04-01

To date, most of invasive cell sheet harvesting methods have used culture surface property variations, such as wettability, pH, electricity, and magnetism, to induce cell detachment. These methods that rely on surface property changes are effective when cell detachment prior to application is necessary, but of limited use when used for cell sheet transfer to target regions. The study reports a new reactive oxygen species (ROS)-induced strategy based on hematoporphyrin-incorporated polyketone film (Hp-PK film) to transfer cell sheets directly to target areas without an intermediate harvesting process. After green LED (510 nm) irradiation, production of exogenous ROS from the Hp-PK films induces cell sheet detachment and transfer. The study suggests that ROS-induced cell detachment property of the Hp-PK film is closely related to conformational changes of extracellular matrix (ECM) proteins. Also, this strategy with the Hp-PK film can be applied by regulating production rate of exogenous ROS in various types of cells, including fibroblasts, mesenchymal stem cells and keratinocytes. In conclusion, ROS-induced method using the Hp-PK film can be used for one-step cell sheet transplantation and has potential in biomedical applications. Copyright © 2018 Elsevier Ltd. All rights reserved.

Evaluation and optimization of SYBR Green real-time reverse transcription polymerase chain reaction as a tool for diagnosis of the Flavivirus genus in Brazil

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Marilia Farignoli Romeiro

2016-06-01

Full Text Available Abstract: INTRODUCTION: The genus Flavivirus includes several pathogenic species that cause severe illness in humans. Therefore, a rapid and accurate molecular method for diagnosis and surveillance of these viruses would be of great importance. Here, we evaluate and optimize a quantitative real-time reverse transcription polymerase chain reaction (RT-PCR method for the diagnosis of the Flavivirus genus. METHODS: We evaluated different commercial kits that use the SYBR Green system for real-time RT-PCR with a primer set that amplifies a fragment of the NS5 flavivirus gene. The specificity and sensitivity of the assay were tested using twelve flaviviruses and ribonucleic acid (RNA transcribed from the yellow fever virus. Additionally, this assay was evaluated using the sera of 410 patients from different regions of Brazil with acute febrile illness and a negative diagnosis for the dengue virus. RESULTS: The real-time RT-PCR amplified all flaviviruses tested at a melting temperature of 79.92 to 83.49°C. A detection limit of 100 copies per ml was determined for this assay. Surprisingly, we detected dengue virus in 4.1% (17/410 of samples from patients with febrile illness and a supposedly negative dengue infection diagnosis. The viral load in patients ranged from 2.1×107to 3.4×103copies per ml. CONCLUSIONS: The real-time RT-PCR method may be very useful for preliminary diagnoses in screenings, outbreaks, and other surveillance studies. Moreover, this assay can be easily applied to monitor viral activity and to measure viral load in pathogenesis studies.

Evaluation and Planning of Urban Green Space Distribution Based on Mobile Phone Data and Two-Step Floating Catchment Area Method

Directory of Open Access Journals (Sweden)

Hao Wu

2018-01-01

Full Text Available Urban green space is closely related to the quality of life of residents. However, the traditional approach to its planning often fails to address its actual service capacity and users’ demand. In this study, facilitated by mobile phone location data, more specific features of the spatial distribution of urban residents are identified. Further, population distribution in relation to traffic analysis zones is mapped. On this basis, the two-step floating catchment area method (2SFCA is adopted in combination with urban green space planning to evaluate the per capita area of green space and its accessibility in practice. Subsequently, classification of per capita area and spatial distribution of green spaces within the study area are obtained; thus, urban districts currently with low accessibility to green areas are identified and can be deemed as key areas for the planning of green areas in the future. The study concludes that mobile phone data can be used to more accurately map the spatial distribution of residents; while, the 2SFCA offers a more comprehensive quantitative measuring of the supply and demand of green spaces. The two combined can be used as an important basis for decision-making in the planning of urban green spaces. Since urban green space can be regarded as a kind of public facility, the methodology of the present study is also believed to be applicable in studies of other types of urban facilities.

A one-step colorimetric acid-base titration sensor using a complementary color changing coordination system.

Science.gov (United States)

Cho, Hui Hun; Kim, Si Hyun; Heo, Jun Hyuk; Moon, Young Eel; Choi, Young Hun; Lim, Dong Cheol; Han, Kwon-Hoon; Lee, Jung Heon

2016-06-21

We report the development of a colorimetric sensor that allows for the quantitative measurement of the acid content via acid-base titration in a single-step. In order to create the sensor, we used a cobalt coordination system (Co-complex sensor) that changes from greenish blue colored Co(H2O)4(OH)2 to pink colored Co(H2O)6(2+) after neutralization. Greenish blue and pink are two complementary colors with a strong contrast. As a certain amount of acid is introduced to the Co-complex sensor, a portion of greenish blue colored Co(H2O)4(OH)2 changes to pink colored Co(H2O)6(2+), producing a different color. As the ratio of greenish blue and pink in the Co-complex sensor is determined by the amount of neutralization reaction occurring between Co(H2O)4(OH)2 and an acid, the sensor produced a spectrum of green, yellow green, brown, orange, and pink colors depending on the acid content. In contrast, the color change appeared only beyond the end point for normal acid-base titration. When we mixed this Co-complex sensor with different concentrations of citric acid, tartaric acid, and malic acid, three representative organic acids in fruits, we observed distinct color changes for each sample. This color change could also be observed in real fruit juice. When we treated the Co-complex sensor with real tangerine juice, it generated diverse colors depending on the concentration of citric acid in each sample. These results provide a new angle on simple but quantitative measurements of analytes for on-site usage in various applications, such as in food, farms, and the drug industry.

Effect of One-Step and Multi-Steps Polishing System on Enamel Roughness

Directory of Open Access Journals (Sweden)

Cynthia Sumali

2013-07-01

Full Text Available Normal 0 false false false MicrosoftInternetExplorer4 The final procedures of orthodontic treatment are bracket debonding and cleaning the remaining adhesive. Multi-step polishing system is the most common method used. The disadvantage of that system is long working time, because of the stages that should be done. Therefore, dental material manufacturer make an improvement to the system, to reduce several stages into one stage only. This new system is known as one-step polishing system. Objective: To compare the effect of one-step and multi-step polishing system on enamel roughness after orthodontic bracket debonding. Methods: Randomized control trial was conducted included twenty-eight maxillary premolar randomized into two polishing system; one-step OptraPol (Ivoclar, Vivadent and multi-step AstroPol (Ivoclar, Vivadent. After bracket debonding, the remaining adhesive on each group was cleaned by subjective polishing system for ninety seconds using low speed handpiece. The enamel roughness was subjected to profilometer, registering two roughness parameters (Ra, Rz. Independent t-test was used to analyze the mean score of enamel roughness in each group. Results: There was no significant difference of enamel roughness between one-step and multi-step polishing system (p>0.005. Conclusion: One-step polishing system can produce a similar enamel roughness to multi-step polishing system after bracket debonding and adhesive cleaning.DOI: 10.14693/jdi.v19i3.136

ONE-STEP AND TWO-STEP CALIBRATION OF A PORTABLE PANORAMIC IMAGE MAPPING SYSTEM

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P.-C. Wang

2012-07-01

Full Text Available A Portable Panoramic Image Mapping System (PPIMS is proposed for rapid acquisition of three-dimensional spatial information. By considering the convenience of use, cost, weight of equipment, precision, and power supply, the designed PPIMS is equipped with 6 circularly arranged cameras to capture panoramic images and a GPS receiver for positioning. The motivation for this design is to develop a hand-held Mobile Mapping System (MMS for some difficult accessing areas by vehicle MMS, such as rugged terrains, forest areas, heavily damaged disaster areas, and crowed places etc. This PPIMS is in fact a GPS assisted close-range photogrammetric system. Compared with the traditional close-range photogrammetry, PPIMS can reduce the need of ground control points significantly. Under the condition of knowing the relative geometric relationships of the equipped sensors, the elements of exterior orientation of each captured image can be solved. However, the procedure of a system calibration should be done accurately to determine the relative geometric relationships of multi-cameras and the GPS antenna center, before the PPIMS can be applied for geo-referenced mapping. In this paper, both of one-step and two-step calibration procedures for PPIMS are performed to determine the lever-arm offsets and boresight angles among cameras and GPS. The performance of the one-step and two-step calibration is evaluated through the analysis of the experimental results. The comparison between these two calibration procedures was also conducted. The two-step calibration method outperforms the one-step calibration method in terms of calibration accuracy and operation convenience. We expect that the proposed two-step calibration procedure can also be applied to other platform-based MMSs.

48 CFR 14.503-1 - Step one.

Science.gov (United States)

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Step one. 14.503-1 Section... AND CONTRACT TYPES SEALED BIDDING Two-Step Sealed Bidding 14.503-1 Step one. (a) Requests for... use the two step method. (3) The requirements of the technical proposal. (4) The evaluation criteria...

Evaluation of one-step luminescent cyanoacrylate fuming.

Science.gov (United States)

Khuu, Alicia; Chadwick, Scott; Spindler, Xanthe; Lam, Rolanda; Moret, Sébastien; Roux, Claude

2016-06-01

One-step luminescent cyanoacrylates have recently been introduced as an alternative to the conventional cyanoacrylate fuming methods. These new techniques do not require the application of a luminescent post-treatment in order to enhance cyanoacrylate-developed fingermarks. In this study, three one-step polymer cyanoacrylates: CN Yellow Crystals (Aneval Inc.), PolyCyano UV (Foster+Freeman Ltd.) and PECA Multiband (BVDA), and one monomer cyanoacrylate: Lumikit™ (Crime Scene Technology), were evaluated against a conventional two-step cyanoacrylate fuming method (Cyanobloom (Foster+Freeman Ltd.) with rhodamine 6G stain). The manufacturers' recommended conditions or conditions compatible with the MVC™ 1000/D (Foster+Freeman Ltd.) were assessed with fingermarks aged for up to 8 weeks on non-porous and semi-porous substrates. Under white light, Cyanobloom generally gave better development than the one-step treatments across the substrates. Similarly when viewed under the respective luminescent conditions, Cyanobloom with rhodamine 6G stain resulted in improved contrast against the one-step treatments except on polystyrene, where PolyCyano UV and PECA Multiband gave better visualisation. Rhodamine 6G post-treatment of one-step samples did not significantly enhance the contrast of any of the one-step treatments against Cyanobloom/rhodamine 6G-treated samples. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

One-step synthesis of DNA functionalized cadmium-free quantum dots and its application in FRET-based protein sensing

Energy Technology Data Exchange (ETDEWEB)

Zhang, Cuiling, E-mail: clzhang@chem.ecnu.edu.cn [Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241 (China); Ding, Caiping [Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241 (China); Zhou, Guohua [School of Chemistry and Chemical Engineering, Lingnan Normal University, Zhanjiang, 524048 (China); Xue, Qin [Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241 (China); Xian, Yuezhong, E-mail: yzxian@chem.ecnu.edu.cn [Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241 (China)

2017-03-08

DNA functionalized quantum dots (QDs) are promising nanoprobes for the fluorescence resonance energy transfer (FRET)-based biosensing. Herein, cadmium-free DNA functionalized Mn-doped ZnS (DNA-ZnS:Mn{sup 2+}) QDs were successfully synthesized by one-step route. As-synthesized QDs show excellent photo-stability with the help of PAA and DNA. Then, we constructed a novel FRET model based on the QDs and WS{sub 2} nanosheets as the energy donor-acceptor pairs, which was successfully applied for the protein detection through the terminal protection of small molecule-linked DNA assay. This work not only explores the potential bioapplication of the DNA-ZnS:Mn{sup 2+} QDs, but also provides a platform for the investigation of small molecule-protein interaction. - Highlights: • The stable and cadmium-free DNA functionalized ZnS:Mn{sup 2+} QDs were successfully synthesized through a facile one-step route. • We constructed a novel FRET system based on one-step synthesized DNA-ZnS:Mn{sup 2+} QDs (donor) and WS{sub 2} nanosheets (acceptor). • The FRET-based strategy was applied for the detection of streptavidin and folate receptor by combining TPSMLD and Exo III.

A single-step method for rapid extraction of total lipids from green microalgae.

Directory of Open Access Journals (Sweden)

Martin Axelsson

Full Text Available Microalgae produce a wide range of lipid compounds of potential commercial interest. Total lipid extraction performed by conventional extraction methods, relying on the chloroform-methanol solvent system are too laborious and time consuming for screening large numbers of samples. In this study, three previous extraction methods devised by Folch et al. (1957, Bligh and Dyer (1959 and Selstam and Öquist (1985 were compared and a faster single-step procedure was developed for extraction of total lipids from green microalgae. In the single-step procedure, 8 ml of a 2∶1 chloroform-methanol (v/v mixture was added to fresh or frozen microalgal paste or pulverized dry algal biomass contained in a glass centrifuge tube. The biomass was manually suspended by vigorously shaking the tube for a few seconds and 2 ml of a 0.73% NaCl water solution was added. Phase separation was facilitated by 2 min of centrifugation at 350 g and the lower phase was recovered for analysis. An uncharacterized microalgal polyculture and the green microalgae Scenedesmus dimorphus, Selenastrum minutum, and Chlorella protothecoides were subjected to the different extraction methods and various techniques of biomass homogenization. The less labour intensive single-step procedure presented here allowed simultaneous recovery of total lipid extracts from multiple samples of green microalgae with quantitative yields and fatty acid profiles comparable to those of the previous methods. While the single-step procedure is highly correlated in lipid extractability (r² = 0.985 to the previous method of Folch et al. (1957, it allowed at least five times higher sample throughput.

One-step microwave synthesis of photoluminescent carbon nanoparticles from sodium dextran sulfate water solution

Science.gov (United States)

Kokorina, Alina A.; Goryacheva, Irina Y.; Sapelkin, Andrei V.; Sukhorukov, Gleb B.

2018-04-01

Photoluminescent (PL) carbon nanoparticles (CNPs) have been synthesized by one-step microwave irradiation from water solution of sodium dextran sulfate (DSS) as the sole carbon source. Microwave (MW) method is very simple and cheap and it provides fast synthesis of CNPs. We have varied synthesis time for obtaining high luminescent CNPs. The synthesized CNPs exhibit excitation-dependent photoluminescent. Final CNPs water solution has a blue- green luminescence. CNPs have low cytotoxicity, good photostability and can be potentially suitable candidates for bioimaging, analysis or analytical tests.

Step-Based Data Sharing and Exchange in One-of-a-Kind Product Collaborative Design for Cloud Manufacturing

Directory of Open Access Journals (Sweden)

B. M. Li

2013-01-01

Full Text Available With the trend for global collaboration, there is a need for collaborative design between geographically distributed teams and companies. In particular, this need is inevitable in the companies doing their business based on one-of-a-kind production (OKP. One important problem is the lack of interoperability and compatibility of data between different CAx systems. This problem is further highlighted in data exchange in cloud manufacturing. To the best of authors' knowledge, current studies have limitations in achieving the interoperability and compatibility of data. In this paper, a STEP-based data model is proposed to represent OKP product data/knowledge, which contains four categories of product knowledge (i.e., customer, product, manufacturing, and resource resp.. A STEP-based data modelling approach is proposed to describe each category of knowledge separately and then connect them to form the final integrated model. Compared with most current product models, this model includes the more complete product data/knowledge involved in OKP product development (OKPPD, and thus it can provide more adequate knowledge support for OKPPD activities. Based on the proposed STEP-based data model, a product data exchange and sharing (DES framework is proposed and developed to enable DES in collaborative OKPPD in the cloud manufacturing environment. Case studies were carried out to validate the proposed data model and DES framework.

ONE STEP GREEN SYNTHESIS OF CARBON QUANTUM DOTS AND ITS APPLICATION TOWARDS THE BIOELECTROANALYTICAL AND BIOLABELING STUDIES

International Nuclear Information System (INIS)

Shereema, Rayammarakkar M.; Sankar, Vandana; Raghu, K.G; Rao, Talasila P.; Shankar, S.Sharath

2015-01-01

Highlights: • A green method was adopted for the synthesis of carbon quantum dots. • Being green fluorescent it was used for the cell imaging. • The carbon paste based carbon quantum dot was fabricated. • This quantum dots/carbon paste electrode was found to be capable of detecting dopamine in the nano molar level. • Possible interference from ascorbic acid and uric acid was successfully eliminated by the fabricated electrode. - Abstract: A green luminescent carbon quantum dots were prepared from maltose by Microwave assisted method followed by passivation with NaOH (pH = 7.4). The TEM measurement confirmed the average size of prepared carbon quantum dots to be 2 nm. Surface characterization such as XPS, FTIR and Raman spectroscopy confirm that the functional groups (C=O, C-OH) were attached on the surface of sp 2 hybridized carbon. Electrochemical characterization studies on carbon paste electrode (CPE) revealed that the synthesized carbon quantum dots showed higher electrocatalytic property, conductivity and surface area. Therefore, herein we report the synthesized carbon quantum dots could find its applicability as an electrochemical sensor for the detection of neurotransmitter, dopamine. Cyclic voltammetry and differential pulse voltammetry (DPV) was employed for the detection of DA in presence of common interferences like UA, and AA with carbon quantum dots modified carbon paste electrode. The developed sensor was effectively applied for the real sample analysis with satisfactory results. Moreover, biological studies in He La cell lines proclaimed that the cell viability was unaffected (100% viability) on incubation with the carbon dots. Significant cellular uptake as revealed by fluorescence imaging makes them suitable for cell labeling studies.

Efficient One-Step Fusion PCR Based on Dual-Asymmetric Primers and Two-Step Annealing

DEFF Research Database (Denmark)

Liu, Yilan; Chen, Jinjin; Thygesen, Anders

2018-01-01

Gene splicing by fusion PCR is a versatile and widely used methodology, especially in synthetic biology. We here describe a rapid method for splicing two fragments by one-round fusion PCR with a dual-asymmetric primers and two-step annealing (ODT) method. During the process, the asymmetric...... intermediate fragments were generated in the early stage. Thereafter, they were hybridized in the subsequent cycles to serve as template for the target full-length product. The process parameters such as primer ratio, elongation temperature and cycle numbers were optimized. In addition, the fusion products...

Ensemble Kalman filtering with one-step-ahead smoothing

KAUST Repository

Raboudi, Naila F.

2018-01-11

The ensemble Kalman filter (EnKF) is widely used for sequential data assimilation. It operates as a succession of forecast and analysis steps. In realistic large-scale applications, EnKFs are implemented with small ensembles and poorly known model error statistics. This limits their representativeness of the background error covariances and, thus, their performance. This work explores the efficiency of the one-step-ahead (OSA) smoothing formulation of the Bayesian filtering problem to enhance the data assimilation performance of EnKFs. Filtering with OSA smoothing introduces an updated step with future observations, conditioning the ensemble sampling with more information. This should provide an improved background ensemble in the analysis step, which may help to mitigate the suboptimal character of EnKF-based methods. Here, the authors demonstrate the efficiency of a stochastic EnKF with OSA smoothing for state estimation. They then introduce a deterministic-like EnKF-OSA based on the singular evolutive interpolated ensemble Kalman (SEIK) filter. The authors show that the proposed SEIK-OSA outperforms both SEIK, as it efficiently exploits the data twice, and the stochastic EnKF-OSA, as it avoids observational error undersampling. They present extensive assimilation results from numerical experiments conducted with the Lorenz-96 model to demonstrate SEIK-OSA’s capabilities.

Rapid detection of Echinococcus species by a high-resolution melting (HRM) approach.

Science.gov (United States)

Santos, Guilherme Brzoskowski; Espínola, Sergio Martín; Ferreira, Henrique Bunselmeyer; Margis, Rogerio; Zaha, Arnaldo

2013-11-14

High-resolution melting (HRM) provides a low-cost, fast and sensitive scanning method that allows the detection of DNA sequence variations in a single step, which makes it appropriate for application in parasite identification and genotyping. The aim of this work was to implement an HRM-PCR assay targeting part of the mitochondrial cox1 gene to achieve an accurate and fast method for Echinococcus spp. differentiation. For melting analysis, a total of 107 samples from seven species were used in this study. The species analyzed included Echinococcus granulosus (n = 41) and Echinococcus ortleppi (n = 50) from bovine, Echinococcus vogeli (n = 2) from paca, Echinococcus oligarthra (n = 3) from agouti, Echinococcus multilocularis (n = 6) from monkey and Echinococcus canadensis (n = 2) and Taenia hydatigena (n = 3) from pig. DNA extraction was performed, and a 444-bp fragment of the cox1 gene was amplified. Two approaches were used, one based on HRM analysis, and a second using SYBR Green Tm-based. In the HRM analysis, a specific profile for each species was observed. Although some species exhibited almost the same melting temperature (Tm) value, the HRM profiles could be clearly discriminated. The SYBR Green Tm-based analysis showed differences between E. granulosus and E. ortleppi and between E. vogeli and E. oligarthra. In this work, we report the implementation of HRM analysis to differentiate species of the genus Echinococcus using part of the mitochondrial gene cox1. This method may be also potentially applied to identify other species belonging to the Taeniidae family.

Amphibious fluorescent carbon dots: one-step green synthesis and application for light-emitting polymer nanocomposites.

Science.gov (United States)

Zhou, Li; He, Benzhao; Huang, Jiachang

2013-09-21

A facile and green approach for the synthesis of amphibious fluorescent carbon dots (CDs) from natural polysaccharide is reported. Light-emitting polymer nanocomposites with excellent optical performance can be easily prepared by incorporation of the amphibious CDs into the polymer matrix.

Shining a light on LAMP assays--a comparison of LAMP visualization methods including the novel use of berberine.

Science.gov (United States)

Fischbach, Jens; Xander, Nina Carolin; Frohme, Marcus; Glökler, Jörn Felix

2015-04-01

The need for simple and effective assays for detecting nucleic acids by isothermal amplification reactions has led to a great variety of end point and real-time monitoring methods. Here we tested direct and indirect methods to visualize the amplification of potato spindle tuber viroid (PSTVd) by loop-mediated isothermal amplification (LAMP) and compared features important for one-pot in-field applications. We compared the performance of magnesium pyrophosphate, hydroxynaphthol blue (HNB), calcein, SYBR Green I, EvaGreen, and berberine. All assays could be used to distinguish between positive and negative samples in visible or UV light. Precipitation of magnesium-pyrophosphate resulted in a turbid reaction solution. The use of HNB resulted in a color change from violet to blue, whereas calcein induced a change from orange to yellow-green. We also investigated berberine as a nucleic acid-specific dye that emits a fluorescence signal under UV light after a positive LAMP reaction. It has a comparable sensitivity to SYBR Green I and EvaGreen. Based on our results, an optimal detection method can be chosen easily for isothermal real-time or end point screening applications.

One step paired electrochemical synthesis of iron and iron oxide nanoparticles

Directory of Open Access Journals (Sweden)

Ordoukhanian Juliet

2016-09-01

Full Text Available In this study, a new one step paired electrochemical method is developed for simultaneous synthesis of iron and iron oxide nanoparticles. iron and iron oxide are prepared as cathodic and anodic products from iron (ii sulfate aqueous solution in a membrane divided electrolytic cell by the pulsed current electrosynthesis. Because of organic solvent-free and electrochemical nature of the synthesis, the process could be considered as green and environmentally friendly. The reduction of energy consumption and low cost are the other significant advantages of this new method that would have a great application potential in the chemical industry. The nanostructure of prepared samples was characterized by Fourier transform infrared spectroscopy (FT-IR, X-ray diffraction (XRD, scanning electron microscopy (SEM and transmission electron microscopy (TEM. The magnetic properties were studied by vibrating sample magnetometer (VsM.

[Application of rapid PCR to authenticate medicinal snakes].

Science.gov (United States)

Chen, Kang; Jiang, Chao; Yuan, Yuan; Huang, Lu-Qi; Li, Man

2014-10-01

To obtained an accurate, rapid and efficient method for authenticate medicinal snakes listed in Chinese Pharmacopoeia (Zaocysd humnades, Bungarus multicinctus, Agkistrodon acutus), a rapid PCR method for authenticate snakes and its adulterants was established based on the classic molecular authentication methods. DNA was extracted by alkaline lysis and the specific primers were amplified by two-steps PCR amplification method. The denatured and annealing temperature and cycle numbers were optimized. When 100 x SYBR Green I was added in the PCR product, strong green fluorescence was visualized under 365 nm UV whereas adulterants without. The whole process can complete in 30-45 minutes. The established method provides the technical support for authentication of the snakes on field.

A Radiation Chemistry Code Based on the Greens Functions of the Diffusion Equation

Science.gov (United States)

Plante, Ianik; Wu, Honglu

2014-01-01

Ionizing radiation produces several radiolytic species such as.OH, e-aq, and H. when interacting with biological matter. Following their creation, radiolytic species diffuse and chemically react with biological molecules such as DNA. Despite years of research, many questions on the DNA damage by ionizing radiation remains, notably on the indirect effect, i.e. the damage resulting from the reactions of the radiolytic species with DNA. To simulate DNA damage by ionizing radiation, we are developing a step-by-step radiation chemistry code that is based on the Green's functions of the diffusion equation (GFDE), which is able to follow the trajectories of all particles and their reactions with time. In the recent years, simulations based on the GFDE have been used extensively in biochemistry, notably to simulate biochemical networks in time and space and are often used as the "gold standard" to validate diffusion-reaction theories. The exact GFDE for partially diffusion-controlled reactions is difficult to use because of its complex form. Therefore, the radial Green's function, which is much simpler, is often used. Hence, much effort has been devoted to the sampling of the radial Green's functions, for which we have developed a sampling algorithm This algorithm only yields the inter-particle distance vector length after a time step; the sampling of the deviation angle of the inter-particle vector is not taken into consideration. In this work, we show that the radial distribution is predicted by the exact radial Green's function. We also use a technique developed by Clifford et al. to generate the inter-particle vector deviation angles, knowing the inter-particle vector length before and after a time step. The results are compared with those predicted by the exact GFDE and by the analytical angular functions for free diffusion. This first step in the creation of the radiation chemistry code should help the understanding of the contribution of the indirect effect in the

Classified one-step high-radix signed-digit arithmetic units

Science.gov (United States)

Cherri, Abdallah K.

1998-08-01

High-radix number systems enable higher information storage density, less complexity, fewer system components, and fewer cascaded gates and operations. A simple one-step fully parallel high-radix signed-digit arithmetic is proposed for parallel optical computing based on new joint spatial encodings. This reduces hardware requirements and improves throughput by reducing the space-bandwidth produce needed. The high-radix signed-digit arithmetic operations are based on classifying the neighboring input digit pairs into various groups to reduce the computation rules. A new joint spatial encoding technique is developed to present both the operands and the computation rules. This technique increases the spatial bandwidth product of the spatial light modulators of the system. An optical implementation of the proposed high-radix signed-digit arithmetic operations is also presented. It is shown that our one-step trinary signed-digit and quaternary signed-digit arithmetic units are much simpler and better than all previously reported high-radix signed-digit techniques.

Safety assessment of green tea based beverages and dried green tea extracts as nutritional supplements.

Science.gov (United States)

Dekant, Wolfgang; Fujii, Kenkichi; Shibata, Eiichiro; Morita, Osamu; Shimotoyodome, Akira

2017-08-05

The safety of green tea infusions and green tea extract (GTE)-based products is reviewed regarding catechins. Epigallocatechin 3-gallate (EGCG), the major catechin present in green tea, is suspected of being responsible for liver toxicity reported in humans consuming food supplements. Intake of EGCG with green tea infusions and GTE-based beverages is up to about 450mg EGCG/person/day in Europe and higher in Asia. Consumption of green tea is not associated with liver damage in humans, and green tea infusion and GTE-based beverages are considered safe in the range of historical uses. In animal studies, EGCG's potency for liver effects is highly dependent on conditions of administration. Use of NOAELs from bolus administration to derive a tolerable upper intake level applying the margin of safety concept results in acceptable EGCG-doses lower than those from one cup of green tea. NOAELs from toxicity studies applying EGCG with diet/split of the daily dose are a better point of departure for risk characterization. In clinical intervention studies, liver effects were not observed after intakes below 600mg EGCG/person/day. Thus, a tolerable upper intake level of 300mg EGCG/person/day is proposed for food supplements; this gives a twofold safety margin to clinical studies that did not report liver effects and a margin of safety of 100 to the NOAELs in animal studies with dietary administration of green tea catechins. Copyright © 2017 Elsevier B.V. All rights reserved.

An Efficient, Eco-friendly and Sustainable One-Pot Synthesis of 3,4-Dihydropyrimidin-2(1H)-ones Directly from Alcohols Catalyzed by Heteropolyanion-Based Ionic Liquids.

Science.gov (United States)

Fu, Renzhong; Yang, Yang; Ma, Xudong; Sun, Yu; Li, Jin; Gao, Hang; Hu, Huaxing; Zeng, Xiaojun; Yi, Jun

2017-09-11

Efficient, eco-friendly and sustainable access to 3,4-dihydropyrimidin-2(1 H )-ones directly from alcohols under microwave and solvent-free conditions has been reported. The practical protocol involves heteropolyanion-based catalyzed oxidation of alcohols to aldehydes with NaNO₃ as the oxidant followed by cyclocondensation with dicarbonyl compounds and urea or thiourea in a two-step, one-pot manner. Compatibility with different functional groups, good to excellent yields and reusable catalysts are the main highlights. The utilization of alcohols instead of aldehydes is a valid and green alternative to the classical Biginelli reaction.

One-step lowrank wave extrapolation

KAUST Repository

Sindi, Ghada Atif

2014-01-01

Wavefield extrapolation is at the heart of modeling, imaging, and Full waveform inversion. Spectral methods gained well deserved attention due to their dispersion free solutions and their natural handling of anisotropic media. We propose a scheme a modified one-step lowrank wave extrapolation using Shanks transform in isotropic, and anisotropic media. Specifically, we utilize a velocity gradient term to add to the accuracy of the phase approximation function in the spectral implementation. With the higher accuracy, we can utilize larger time steps and make the extrapolation more efficient. Applications to models with strong inhomogeneity and considerable anisotropy demonstrates the utility of the approach.

AFLP fragment isolation technique as a method to produce random sequences for single nucleotide polymorphism discovery in the green turtle, Chelonia mydas.

Science.gov (United States)

Roden, Suzanne E; Dutton, Peter H; Morin, Phillip A

2009-01-01

The green sea turtle, Chelonia mydas, was used as a case study for single nucleotide polymorphism (SNP) discovery in a species that has little genetic sequence information available. As green turtles have a complex population structure, additional nuclear markers other than microsatellites could add to our understanding of their complex life history. Amplified fragment length polymorphism technique was used to generate sets of random fragments of genomic DNA, which were then electrophoretically separated with precast gels, stained with SYBR green, excised, and directly sequenced. It was possible to perform this method without the use of polyacrylamide gels, radioactive or fluorescent labeled primers, or hybridization methods, reducing the time, expense, and safety hazards of SNP discovery. Within 13 loci, 2547 base pairs were screened, resulting in the discovery of 35 SNPs. Using this method, it was possible to yield a sufficient number of loci to screen for SNP markers without the availability of prior sequence information.

One Step Hydrogen Generation Through Sorption Enhanced Reforming

Energy Technology Data Exchange (ETDEWEB)

Mays, Jeff [Gas Technology Inst., Des Plaines, IL (United States)

2017-08-03

One-step hydrogen generation, using Sorption Enhanced Reforming (SER) technology, is an innovative means of providing critical energy and environmental improvements to US manufacturing processes. The Gas Technology Institute (GTI) is developing a Compact Hydrogen Generator (CHG) process, based on SER technology, which successfully integrates previously independent process steps, achieves superior energy efficiency by lowering reaction temperatures, and provides pathways to doubling energy productivity with less environmental pollution. GTI’s prior CHG process development efforts have culminated in an operational pilot plant. During the initial pilot testing, GTI identified two operating risks- 1) catalyst coating with calcium aluminate compounds, 2) limited solids handling of the sorbent. Under this contract GTI evaluated alternative materials (one catalyst and two sorbents) to mitigate both risks. The alternate catalyst met performance targets and did not experience coating with calcium aluminate compounds of any kind. The alternate sorbent materials demonstrated viable operation, with one material enabling a three-fold increase in sorbent flow. The testing also demonstrated operation at 90% of its rated capacity. Lastly, a carbon dioxide co-production study was performed to assess the advantage of the solid phase separation of carbon dioxide- inherent in the CHG process. Approximately 70% lower capital cost is achievable compared to SMR-based hydrogen production with CO2 capture, as well as improved operating costs.

Comparison of the Screening Tests for Gestational Diabetes Mellitus between "One-Step" and "Two-Step" Methods among Thai Pregnant Women.

Science.gov (United States)

Luewan, Suchaya; Bootchaingam, Phenphan; Tongsong, Theera

2018-01-01

To compare the prevalence and pregnancy outcomes of GDM between those screened by the "one-step" (75 gm GTT) and "two-step" (100 gm GTT) methods. A prospective study was conducted on singleton pregnancies at low or average risk of GDM. All were screened between 24 and 28 weeks, using the one-step or two-step method based on patients' preference. The primary outcome was prevalence of GDM, and secondary outcomes included birthweight, gestational age, rates of preterm birth, small/large-for-gestational age, low Apgar scores, cesarean section, and pregnancy-induced hypertension. A total of 648 women were screened: 278 in the one-step group and 370 in the two-step group. The prevalence of GDM was significantly higher in the one-step group; 32.0% versus 10.3%. Baseline characteristics and pregnancy outcomes in both groups were comparable. However, mean birthweight was significantly higher among pregnancies with GDM diagnosed by the two-step approach (3204 ± 555 versus 3009 ± 666 g; p =0.022). Likewise, the rate of large-for-date tended to be higher in the two-step group, but was not significant. The one-step approach is associated with very high prevalence of GDM among Thai population, without clear evidence of better outcomes. Thus, this approach may not be appropriate for screening in a busy antenatal care clinic like our setting or other centers in developing countries.

Stability study in one step steam generators

International Nuclear Information System (INIS)

Abbate, P.

1990-01-01

The TWO program is presented developed for the behaviour limit calculation stable in one step steam generators for the case of Density Waves phenomenom. The program is based on a nodal model which, using Laplace transformation equations, allows to study the system's transfer functions and foresee the beginning of the unstable behaviour. This program has been satisfactorily validated against channels data uniformly heated in the range from 4.0 to 6.0 Mpa. Results on the CAREM reactor's steam generator analysis are presented. (Author) [es

The one-loop Green's functions of dimensionally reduced gauge theories

International Nuclear Information System (INIS)

Ketov, S.V.; Prager, Y.S.

1988-01-01

The dimensional regularization technique as well as that by dimensional reduction is applied to the calculation of the regularized one-loop Green's functions in dsub(o)-dimensional Yang-Mills theory with real massless scalars and spinors in arbitrary (real) representations of a gauge group G. As a particular example, the super-symmetrically regularized one-loop Green's functions of the N=4 supersymmetric Yang-Mills model are derived. (author). 17 refs

A novel one-step Helicobacter pylori saliva antigen test.

Science.gov (United States)

Yang, Bi-Ling; Yeh, Chun; Kwong, Wei-Gang; Lee, Shou-Dong

2015-02-01

A rapid, reliable, and sufficiently accurate test for diagnosing Helicobacter pylori infection is required for screening dyspeptic patients before a referral for endoscopy. The purpose of this article is two-fold: first, to evaluate the accuracy of a one-step H. pylori saliva antigen (HPS) test; and second, to compare noninvasive and invasive H. pylori tests in Taiwanese population. A total of 104 consecutive dyspeptic patients admitted for gastroenterology into the outpatient department underwent a one-step HPS test, rapid urease test, histology, and (13)C-urea breath test (13)C-UBT (proto C-13 urea kit). The accuracy of the HPS test was compared with a gold standard defined by at least two positive H. pylori test results from three H. pylori tests (histology, rapid urease test, and (13)C-UBT). The 104 patients eligible for analysis (mean age: 58 years, range 22-87 years), 21 (20%) were gold standard positive. Among them, the positive of the one-step H. pylori saliva Ag test, rapid urease test, (13)C-UBT, histology were (52; 50%), (17; 16%), (27; 25%) and (22; 21%) respectively. The sensitivity and specificity of the HPS tests, rapid urease test, (13)C-UBTs, and histology were 71.43% and 55.42%, 76.19% and 98.80%, 100% and 92.77%, and 85.71% and 95.18%, respectively, relative to the gold standard. The one-step HPS test exhibited a sensitivity of 71.43%, nearly equivalent to that of the rapid urea test. The one-step HPS test exhibited a high sensitivity and low specificity compared with the other tests, indicating that it is not sufficiently accurate for use in a clinical setting for diagnosing H. pylori infection. However, the test is simple to use (requiring only a saliva sample), inexpensive, and noninvasive in its application, and thus appealing for use in population-based prevalence surveys of the epidemiology of H. pylori infection. Copyright © 2014. Published by Elsevier Taiwan.

One-Step-Ahead Predictive Control for Hydroturbine Governor

Directory of Open Access Journals (Sweden)

Zhihuai Xiao

2015-01-01

Full Text Available The hydroturbine generator regulating system can be considered as one system synthetically integrating water, machine, and electricity. It is a complex and nonlinear system, and its configuration and parameters are time-dependent. A one-step-ahead predictive control based on on-line trained neural networks (NNs for hydroturbine governor with variation in gate position is described in this paper. The proposed control algorithm consists of a one-step-ahead neuropredictor that tracks the dynamic characteristics of the plant and predicts its output and a neurocontroller to generate the optimal control signal. The weights of two NNs, initially trained off-line, are updated on-line according to the scalar error. The proposed controller can thus track operating conditions in real-time and produce the optimal control signal over the wide operating range. Only the inputs and outputs of the generator are measured and there is no need to determine the other states of the generator. Simulations have been performed with varying operating conditions and different disturbances to compare the performance of the proposed controller with that of a conventional PID controller and validate the feasibility of the proposed approach.

Variable order one-step methods for initial value problems I ...

African Journals Online (AJOL)

A class of variable order one-step integrators is proposed for Initial Value Problems (IVPs) in Ordinary Differential Equations (ODEs). It is based on a rational interpolant. Journal of the Nigerian Association of Mathematical Physics Vol. 10 2006: pp. 91-96 ...

Aptamer-fluorescent silica nanoparticles bioconjugates based dual-color flow cytometry for specific detection of Staphylococcus aureus.

Science.gov (United States)

He, Xiaoxiao; Li, Yuhong; He, Dinggen; Wang, Kemin; Shangguan, Jingfang; Shi, Hui

2014-07-01

This paper describes a sensitive and specific determination strategy for Staphylococcus aureus (S. aureus) detection using aptamer recognition and fluorescent silica nanoparticles (FSiNPs) label based dual-color flow cytometry assay (Aptamer/FSiNPs-DCFCM). In the protocol, an aptamer, having high affinity to S. aureus, was first covalently immobilized onto chloropropyl functionalized FSiNPs through a click chemistry approach to generate aptamer-nanoparticles bioconjugates (Aptamer/FSiNPs). Next, S. aureus was incubated with Aptamer/FSiNPs, and then stained with SYBR Green I (a special staining material for the duplex DNA). Upon target binding and nucleic acid staining with SYBR Green I, the S. aureus was determined using two-color flow cytometry. The method took advantage of the specificity of aptamer, signal amplification of FSiNPs label and decreased false positives of two-color flow cytometry assay. It was demonstrated that these Aptamer/FSiNPs could efficiently recognize and fluorescently label target S. aureus. Through multiparameter determination with flow cytometry, this assay allowed for detection of as low as 1.5 x 10(2) and 7.6 x 10(2) cells mL(-1) S. aureus in buffer and spiked milk, respectively, with higher sensitivity than the Aptamer/FITC based flow cytometry.

Anti-idiotypic nanobody-alkaline phosphatase fusion proteins: Development of a one-step competitive enzyme immunoassay for fumonisin B_1 detection in cereal

International Nuclear Information System (INIS)

Shu, Mei; Xu, Yang; Liu, Xing; Li, Yanping; He, Qinghua; Tu, Zhui; Fu, Jinheng; Gee, Shirley J.; Hammock, Bruce D.

2016-01-01

A rapid and sensitive one-step competitive enzyme immunoassay for the detection of FB_1 was developed. The anti-idiotypic nanobody–alkaline phosphatase (Ab2β−Nb−AP) was validated by the AP enzyme activity and the properties of bounding to anti-FB1-mAb (3F11) through colorimetric and chemiluminescence analyses. The 50% inhibitory concentration and the detection limit (LOD) of colorimetric enzyme-linked immunosorbent assay (ELISA) for FB_1 were 2.69 and 0.35 ng mL"−"1, respectively, with a linear range of 0.93–7.73 ng mL"−"1. The LOD of the chemiluminescence ELISA (CLIA) was 0.12 ng mL"−"1, and the IC_5_0 was 0.89 ± 0.09 ng mL"−"1 with a linear range of 0.29–2.68 ng mL"−"1. Compared with LC-MS/MS, the results of this assay indicated the reliability of the Ab2β−Nb−AP fusion protein based one-step competitive immunoassay for monitoring FB_1 contamination in cereals. The Ab2β−Nb−AP fusion proteins have the potential to replace chemically-coupled probes in competitive enzyme immunoassay systems. - Highlights: • Ab2β−Nb−AP has the potential to replace chemically-coupled probes. • Ab2β−Nb−AP is homogeneous enzyme-labelled antigen can be prepared reproducibly. • We developed a green and rapid one-step competitive enzyme immunoassay. • The sensitivity of one-step CLIA was 9-folds higher than two-step ELISA.

Amperometric sensor for ethanol based on one-step electropolymerization of thionine-carbon nanofiber nanocomposite containing alcohol oxidase.

Science.gov (United States)

Wu, Lina; McIntosh, Mike; Zhang, Xueji; Ju, Huangxian

2007-12-15

Thionine had strong interaction with carbon nanofiber (CNF) and was used in the non-covalent functionalization of carbon nanofiber for the preparation of stable thionine-CNF nanocomposite with good dispersion. With a simple one-step electrochemical polymerization of thionine-CNF nanocomposite and alcohol oxidase (AOD), a stable poly(thionine)-CNF/AOD biocomposite film was formed on electrode surface. Based on the excellent catalytic activity of the biocomposite film toward reduction of dissolved oxygen, a sensitive ethanol biosensor was proposed. The ethanol biosensor could monitor ethanol ranging from 2.0 to 252 microM with a detection limit of 1.7 microM. It displayed a rapid response, an expanded linear response range as well as excellent reproducibility and stability. The combination of catalytic activity of CNF and the promising feature of the biocomposite with one-step non-manual technique favored the sensitive determination of ethanol with improved analytical capabilities.

Step one within stepped care trauma-focused cognitive behavioral therapy for young children: a pilot study.

Science.gov (United States)

Salloum, Alison; Robst, John; Scheeringa, Michael S; Cohen, Judith A; Wang, Wei; Murphy, Tanya K; Tolin, David F; Storch, Eric A

2014-02-01

This pilot study explored the preliminary efficacy, parent acceptability and economic cost of delivering Step One within Stepped Care Trauma-Focused Cognitive Behavioral Therapy (SC-TF-CBT). Nine young children ages 3-6 years and their parents participated in SC-TF-CBT. Eighty-three percent (5/6) of the children who completed Step One treatment and 55.6 % (5/9) of the intent-to-treat sample responded to Step One. One case relapsed at post-assessment. Treatment gains were maintained at 3-month follow-up. Generally, parents found Step One to be acceptable and were satisfied with treatment. At 3-month follow-up, the cost per unit improvement for posttraumatic stress symptoms and severity ranged from $27.65 to $131.33 for the responders and from $36.12 to $208.11 for the intent-to-treat sample. Further research on stepped care for young children is warranted to examine if this approach is more efficient, accessible and cost-effective than traditional therapy.

Synthesis and biological evaluation of some novel pyrido[1,2-a]pyrimidin-4-ones as antimalarial agents.

Science.gov (United States)

Mane, Uttam R; Mohanakrishnan, D; Sahal, Dinkar; Murumkar, Prashant R; Giridhar, Rajani; Yadav, Mange Ram

2014-05-22

Novel pyrido[1,2-a]pyrimidin-4-ones have been synthesized and evaluated for their antimalarial activity by SYBR Green I assay against erythrocytic stages of chloroquine (CQ) sensitive Pf 3D7 strain. The antimalarial screening of 42 different compounds revealed that 3-Fluorobenzyl(4-oxo-4H-pyrido [1,2-a]pyrimidin-3-yl)carbamate (21, IC50 value 33 μM) and 4-Oxo-N-[4-(trifluoromethyl)benzyl]-4H-pyrido[1,2-a]pyrimidine-3-carboxamide (37, IC50 value 37 μM) showed moderate antimalarial activity. Cytotoxicity study was performed against mammalian cell line (Huh-7) by using the MTT assay for the moderately active compounds. Structural activity relationship (SAR) studies displayed that B-ring unsubstituted pyrido[1,2-a]pyrimidine scaffold is responsible for the antimalarial activities of the evaluated derivatives. This SAR based antimalarial screening supported that pyrido[1,2-a]pyrimidin-4-one can be considered as a lead heterocyclic structure for further development of more potent derivatives for antimalarial activity. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

Marginal microleakage of class V resin-based composite restorations bonded with six one-step self-etch systems

Directory of Open Access Journals (Sweden)

Alfonso Sánchez-Ayala

2013-06-01

Full Text Available This study compared the microleakage of class V restorations bonded with various one-step self-etching adhesives. Seventy class V resin-based composite restorations were prepared on the buccal and lingual surfaces of 35 premolars, by using: Clearfil S 3 Bond, G-Bond, iBond, One Coat 7.0, OptiBond All-In-One, or Xeno IV. The Adper Single Bond etch-and-rinse two-step adhesive was employed as a control. Specimens were thermocycled for 500 cycles in separate water baths at 5°C and 55°C and loaded under 40 to 70 N for 50,000 cycles. Marginal microleakage was measured based on the penetration of a tracer agent. Although the control showed no microleakage at the enamel margins, there were no differences between groups (p = 0.06. None of the adhesives avoided microleakage at the dentin margins, and they displayed similar performances (p = 0.76. When both margins were compared, iBond® presented higher microleakage (p < 0.05 at the enamel margins (median, 1.00; Q3–Q1, 1.25–0.00 compared to the dentin margins (median, 0.00; Q3–Q1, 0.25–0.00. The study adhesives showed similar abilities to seal the margins of class V restorations, except for iBond®, which presented lower performance at the enamel margin.

Effects of computer-based graphic organizers to solve one-step word problems for middle school students with mild intellectual disability: A preliminary study.

Science.gov (United States)

Sheriff, Kelli A; Boon, Richard T

2014-08-01

The purpose of this study was to examine the effects of computer-based graphic organizers, using Kidspiration 3© software, to solve one-step word problems. Participants included three students with mild intellectual disability enrolled in a functional academic skills curriculum in a self-contained classroom. A multiple probe single-subject research design (Horner & Baer, 1978) was used to evaluate the effectiveness of computer-based graphic organizers to solving mathematical one-step word problems. During the baseline phase, the students completed a teacher-generated worksheet that consisted of nine functional word problems in a traditional format using a pencil, paper, and a calculator. In the intervention and maintenance phases, the students were instructed to complete the word problems using a computer-based graphic organizer. Results indicated that all three of the students improved in their ability to solve the one-step word problems using computer-based graphic organizers compared to traditional instructional practices. Limitations of the study and recommendations for future research directions are discussed. Copyright © 2014 Elsevier Ltd. All rights reserved.

Morphology-photovoltaic property correlation in perovskite solar cells: One-step versus two-step deposition of CH3NH3PbI3

Directory of Open Access Journals (Sweden)

Jeong-Hyeok Im

2014-08-01

Full Text Available Perovskite CH3NH3PbI3 light absorber is deposited on the mesoporous TiO2 layer via one-step and two-step coating methods and their photovoltaic performances are compared. One-step coating using a solution containing CH3NH3I and PbI2 shows average power conversion efficiency (PCE of 7.5%, while higher average PCE of 13.9% is obtained from two-step coating method, mainly due to higher voltage and fill factor. The coverage, pore-filling, and morphology of the deposited perovskite are found to be critical in photovoltaic performance of the mesoporous TiO2 based perovskite solar cells.

Biomass-based magnetic fluorescent nanoparticles: One-step scalable synthesis, application as drug carriers and mechanism study.

Science.gov (United States)

Li, Lei; Wang, Feijun; Shao, Ziqiang

2018-03-15

A biomass-based magnetic fluorescent nanoparticle (MFNPs) was successively in situ synthesized via a one-step high-gravity approach, which constructed by a magnetic core of Fe 3 O 4 nanoparticles, the fluorescent marker of carbon dots (CDs), and shells of chitosan (CS). The obtained MFNPs had a 10 nm average diameter and narrow particle size distribution, low cytotoxicity, superior fluorescent emission and superparamagnetic properties. The encapsulating and release 5-fluorouracil experiments confirmed that the introduction of CS/CDs effectively improved the drug loading capacity. Mechanism and kinetic studies proved that: (i) the monolayer adsorption was the main sorption mode under the studied conditions; (ii) the whole adsorption process was controlled by intra-liquid diffusion mass transfer and governed by chemisorption; and (iii) the release process was controlled by Fickian diffusion. These results demonstrated this method to one-step continuously produce MFNPs and the construction of non-toxic nanostructure possessed great superiority in currently Nano-delivery systems, which would show high application value in targeted drug delivery, magnetic fluid hyperthermia treatment, magnetic resonance imaging (MRI), in vitro testing and relative research. Copyright © 2017 Elsevier Ltd. All rights reserved.

Incidence, risk factors, and pregnancy outcomes of gestational diabetes mellitus using one-step versus two-step diagnostic approaches: A population-based cohort study in Isfahan, Iran.

Science.gov (United States)

Hosseini, Elham; Janghorbani, Mohsen; Aminorroaya, Ashraf

2018-06-01

To study the incidence, risk factors, and pregnancy outcomes associated with gestational diabetes mellitus (GDM) diagnosed with one-step and two-step screening approaches. 1000 pregnant women who were eligible and consented to participate underwent fasting plasma glucose testing at the first prenatal visit (6-14 weeks). The women free from GDM or overt diabetes were screened at 24-28 weeks using the 50-g glucose challenge test (GCT) followed by 100-g, 3-h oral glucose tolerance test (OGTT) (two-step method). Regardless of the GCT result, all women underwent a 75-g, 2-h OGTT within one-week interval (one-step method). GDM incidence using the one-step and two-step methods was 9.3% (95% CI: 7.4-11.2) and 4.2% (95% CI: 2.9-5.5). GDM significantly increased the risk of macrosomia, gestational hypertension, preeclampsia, and cesarean section and older age and family history of diabetes significantly increased the risk of developing GDM in both approaches. In two-step method, higher pre-pregnancy body mass index and lower physical activity during pregnancy along with higher earlier cesarean section also increased significantly the risk of developing GDM. Despite a higher incidence of GDM using the one-step approach, more risk factors for and a stronger effect of GDM on adverse pregnancy outcomes were found when using the two-step approach. Longer follow-up of women with and without GDM may change the results using both approaches. Copyright © 2018 Elsevier B.V. All rights reserved.

A novel one-step Helicobacter pylori saliva antigen test

Directory of Open Access Journals (Sweden)

Bi-Ling Yang

2015-02-01

Conclusion: The one-step HPS test exhibited a high sensitivity and low specificity compared with the other tests, indicating that it is not sufficiently accurate for use in a clinical setting for diagnosing H. pylori infection. However, the test is simple to use (requiring only a saliva sample, inexpensive, and noninvasive in its application, and thus appealing for use in population-based prevalence surveys of the epidemiology of H. pylori infection.

Evaluation of a real-time PCR assay based on the single-copy SAG1 gene for the detection of Toxoplasma gondii.

Science.gov (United States)

Yu, Haijie; Huang, Bin; Zhuo, Xunhui; Chen, Xueqiu; Du, Aifang

2013-11-08

Real-time PCR-based detection of Toxoplasma gondii is very sensitive and convenient for diagnosing toxoplasmosis. However, the performance of the PCR assays could be influenced by the target gene chosen. Here we evaluate a real-time PCR assay using double-stranded DNA dyes (SYBR(®) Green I assay) with a new set of primers targeting the SAG1 gene for the fast and specific detection of T. gondii. The assay showed higher sensitivity than conventional PCR protocols using T. gondii DNA as template. The detection limit of the developed real-time PCR assay was in the order of 1 tachyzoite. The assay was also assessed by experimentally infected mice and showed positive results for blood (25%), spleen (50%) and lung (50%) as early as 1 dpi. The specificity of the assay was confirmed by using DNA from Neospora caninum, Escherichia coli, Babesia bovis, Trypanosoma brucei, Cryptosporidium parvum, and Toxocara canis. Assay applicability was successfully tested in blood samples collected from slaughtered pigs. These results indicate that, based on SYBR(®) green I, the quantitative SAG1 assay may also be useful in the study of the pathogenicity, immunoprophylaxis, and treatment of T. gondii. Copyright © 2013 Elsevier B.V. All rights reserved.

One-step method for the production of nanofluids

Science.gov (United States)

Kostic, Milivoje [Chicago, IL; Golubovic, Mihajlo [Chicago, IL; Hull, John R [Downers Grove, IL; Choi, Stephen U. S. [Napersville, IL

2010-05-18

A one step method and system for producing nanofluids by a particle-source evaporation and deposition of the evaporant into a base fluid. The base fluid such (i.e. ethylene glycol) is placed in a rotating cylindrical drum having an adjustable heater-boat-evaporator and heat exchanger-cooler apparatus. As the drum rotates, a thin liquid layer is formed on the inside surface of the drum. A heater-boat-evaporator having an evaporant material (particle-source) placed within its boat evaporator is adjustably positioned near a portion of the rotating thin liquid layer, the evaporant material being heated thereby evaporating a portion of the evaporant material, the evaporated material absorbed by the liquid film to form nanofluid.

One step beyond: Different step-to-step transitions exist during continuous contact brachiation in siamangs

Directory of Open Access Journals (Sweden)

Fana Michilsens

2012-02-01

In brachiation, two main gaits are distinguished, ricochetal brachiation and continuous contact brachiation. During ricochetal brachiation, a flight phase exists and the body centre of mass (bCOM describes a parabolic trajectory. For continuous contact brachiation, where at least one hand is always in contact with the substrate, we showed in an earlier paper that four step-to-step transition types occur. We referred to these as a ‘point’, a ‘loop’, a ‘backward pendulum’ and a ‘parabolic’ transition. Only the first two transition types have previously been mentioned in the existing literature on gibbon brachiation. In the current study, we used three-dimensional video and force analysis to describe and characterize these four step-to-step transition types. Results show that, although individual preference occurs, the brachiation strides characterized by each transition type are mainly associated with speed. Yet, these four transitions seem to form a continuum rather than four distinct types. Energy recovery and collision fraction are used as estimators of mechanical efficiency of brachiation and, remarkably, these parameters do not differ between strides with different transition types. All strides show high energy recoveries (mean  = 70±11.4% and low collision fractions (mean  = 0.2±0.13, regardless of the step-to-step transition type used. We conclude that siamangs have efficient means of modifying locomotor speed during continuous contact brachiation by choosing particular step-to-step transition types, which all minimize collision fraction and enhance energy recovery.

Building HIA approaches into strategies for green space use: an example from Plymouth's (UK) Stepping Stones to Nature project.

Science.gov (United States)

Richardson, J; Goss, Z; Pratt, A; Sharman, J; Tighe, M

2013-12-01

The health and well-being benefits of access to green space are well documented. Research suggests positive findings regardless of social group, however barriers exist that limit access to green space, including proximity, geography and differing social conditions. Current public health policy aims to broaden the range of environmental public health interventions through effective partnership working, providing opportunities to work across agencies to promote the use of green space. Health Impact Assessment (HIA) is a combination of methods and procedures to assess the potential health and well-being impacts of policies, developments and projects. It provides a means by which negative impacts can be mitigated and positive impacts can be enhanced, and has potential application for assessing green space use. This paper describes the application of a HIA approach to a multi-agency project (Stepping Stones to Nature--SS2N) in the UK designed to improve local green spaces and facilitate green space use in areas classified as having high levels of deprivation. The findings suggest that the SS2N project had the potential to provide significant positive benefits in the areas of physical activity, mental and social well-being. Specific findings for one locality identified a range of actions that could be taken to enhance benefits, and mitigate negative factors such as anti-social behaviour. The HIA approach proved to be a valuable process through which impacts of a community development/public health project could be enhanced and negative impacts prevented at an early stage; it illustrates how a HIA approach could enhance multi-agency working to promote health and well-being in communities.

Dynamic cholesteric liquid crystal superstructures photoaligned by one-step polarization holography

Science.gov (United States)

Li, Sen-Sen; Shen, Yuan; Chang, Zhen-Ni; Li, Wen-Song; Xu, Yan-Chao; Fan, Xing-Yu; Chen, Lu-Jian

2017-12-01

A convenient approach to modulate the fingerprint textures of methyl red (MR) doped cholesteric liquid crystals by asymmetric photoalignment in the green-light waveband is presented, resulting in the generation of voltage-controllable helical superstructures. The interaction between the MR molecules and the incident light polarization determines the initial twisted planar geometry, providing a multivariant control over the stripe directions of fingerprint textures by applying a proper electric field. The key factors for precise manipulation of fingerprint stripes in a predictable and rewritable manner are analyzed theoretically and investigated experimentally, which involves the alignment asymmetry, the ratio of cell gap to natural pitch length, and the chirality of chiral dopant. Dynamic periodic fingerprint textures in shapes of dashed curve and dashed line are further demonstrated by utilizing a facile one-step polarization holography process using two beams with orthogonal circular and orthogonal linear polarizations, respectively. It is believed that the practical approach described in this study would enrich the research contents of self-assembled hierarchical superstructures using soft liquid crystal building blocks.

Relative sensitivity of conventional and real-time PCR assays for detection of SFG Rickettsia in blood and tissue samples from laboratory animals.

Science.gov (United States)

Zemtsova, Galina E; Montgomery, Merrill; Levin, Michael L

2015-01-01

Studies on the natural transmission cycles of zoonotic pathogens and the reservoir competence of vertebrate hosts require methods for reliable diagnosis of infection in wild and laboratory animals. Several PCR-based applications have been developed for detection of infections caused by Spotted Fever group Rickettsia spp. in a variety of animal tissues. These assays are being widely used by researchers, but they differ in their sensitivity and reliability. We compared the sensitivity of five previously published conventional PCR assays and one SYBR green-based real-time PCR assay for the detection of rickettsial DNA in blood and tissue samples from Rickettsia- infected laboratory animals (n = 87). The real-time PCR, which detected rickettsial DNA in 37.9% of samples, was the most sensitive. The next best were the semi-nested ompA assay and rpoB conventional PCR, which detected as positive 18.4% and 14.9% samples respectively. Conventional assays targeting ompB, gltA and hrtA genes have been the least sensitive. Therefore, we recommend the SYBR green-based real-time PCR as a tool for the detection of rickettsial DNA in animal samples due to its higher sensitivity when compared to more traditional assays.

A nanosilver-based spectrophotometric method for determination of malachite green in surface water samples.

Science.gov (United States)

Sahraei, R; Farmany, A; Mortazavi, S S; Noorizadeh, H

2013-07-01

A new spectrophotometric method is reported for the determination of nanomolar level of malachite green in surface water samples. The method is based on the catalytic effect of silver nanoparticles on the oxidation of malachite green by hexacyanoferrate (III) in acetate-acetic acid medium. The absorbance is measured at 610 nm with the fixed-time method. Under the optimum conditions, the linear range was 8.0 × 10(-9)-2.0 × 10(-7) mol L(-1) malachite green with a correlation coefficient of 0.996. The limit of detection (S/N = 3) was 2.0 × 10(-9) mol L(-1). Relative standard deviation for ten replicate determinations of 1.0 × 10(-8) mol L(-1) malachite green was 1.86%. The method is featured with good accuracy and reproducibility for malachite green determination in surface water samples without any pre-concentration and separation step.

Synthesis and characterization of copper nanofluid by a novel one-step method

International Nuclear Information System (INIS)

Kumar, S. Ananda; Meenakshi, K. Shree; Narashimhan, B.R.V.; Srikanth, S.; Arthanareeswaran, G.

2009-01-01

This paper presents a novel one-step method for the preparation of stable, non-agglomerated copper nanofluids by reducing copper sulphate pentahydrate with sodium hypophosphite as reducing agent in ethylene glycol as base fluid by means of conventional heating. This is an in situ, one-step method which gives high yield of product with less time consumption. The characterization of the nanofluid is done by particle size analyzer, X-ray diffraction topography, UV-vis analysis and Fourier transform infrared spectroscopy (FT-IR) followed by the study of thermal conductivity of nanofluid by the transient hot wire method

Opportunities for green growth; Vihreaen kasvun mahdollisuudet

Energy Technology Data Exchange (ETDEWEB)

Antikainen, R.; Mickwitz, P.; Seppaelae, J. [and others

2013-03-15

The report seeks an answer to the question as to the kind of policy decisions (steps) by which preconditions for green growth may be created in Finland. The proposed steps are based on a review of earlier research and studies relating to Finland's key consumption and production systems (food, housing, transport and energy) and to certain path finding countries in terms of the green economy (the Netherlands, Germany, Sweden, Brazil). In addition, the report examines various models by which systemic change towards a green economy may be supported. The report also highlights successful examples of green business activity and measures to promote green growth. Green Growth is defined as low-carbon, resource-efficient economic growth based on safeguarding the functional capacity of ecosystems while promoting wellbeing and social justice. Green growth is considered to have significant worldwide potential, which is currently evident particularly in the rapid growth of cleantech demand. Successful future actors will be more material- and energy-efficient than their competitors, and they will be able to provide services and products flexibly for a low-carbon society. There are opportunities for green growth in all sectors of society. Green growth may consist of an entirely new kind of business activity and create new companies, but there are also opportunities in our traditional energy- and resource-intensive industries. Companies have a key role in growth, but realising green growth also requires changes in consumption. Central, regional and local government will act as facilitators in creating the preconditions for green growth. The report presents a number of policy measures and processes by which Finland can support green growth. Proposals for steps towards green growth include: (A.) Creating preconditions for green growth through a joint vision and political commitment. (B.) Stimulating companies' green growth potential and boosting green demand. (C

Anti-idiotypic nanobody-alkaline phosphatase fusion proteins: Development of a one-step competitive enzyme immunoassay for fumonisin B{sub 1} detection in cereal

Energy Technology Data Exchange (ETDEWEB)

Shu, Mei [State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047 (China); Jiangxi-OAI Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047 (China); Xu, Yang, E-mail: xuyang@ncu.edu.cn [State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047 (China); Jiangxi-OAI Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047 (China); Liu, Xing [State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047 (China); College of Food Science and Technology, Hainan University, No. 58 Renmin Avenue, Haikou 570228 (China); Li, Yanping; He, Qinghua [Jiangxi-OAI Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047 (China); Tu, Zhui [State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047 (China); Fu, Jinheng [Jiangxi-OAI Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047 (China); Gee, Shirley J.; Hammock, Bruce D. [Department of Entomology and UCD Comprehensive Cancer Center, University of California, Davis, CA 95616 (United States)

2016-06-14

A rapid and sensitive one-step competitive enzyme immunoassay for the detection of FB{sub 1} was developed. The anti-idiotypic nanobody–alkaline phosphatase (Ab2β−Nb−AP) was validated by the AP enzyme activity and the properties of bounding to anti-FB1-mAb (3F11) through colorimetric and chemiluminescence analyses. The 50% inhibitory concentration and the detection limit (LOD) of colorimetric enzyme-linked immunosorbent assay (ELISA) for FB{sub 1} were 2.69 and 0.35 ng mL{sup −1}, respectively, with a linear range of 0.93–7.73 ng mL{sup −1}. The LOD of the chemiluminescence ELISA (CLIA) was 0.12 ng mL{sup −1}, and the IC{sub 50} was 0.89 ± 0.09 ng mL{sup −1} with a linear range of 0.29–2.68 ng mL{sup −1}. Compared with LC-MS/MS, the results of this assay indicated the reliability of the Ab2β−Nb−AP fusion protein based one-step competitive immunoassay for monitoring FB{sub 1} contamination in cereals. The Ab2β−Nb−AP fusion proteins have the potential to replace chemically-coupled probes in competitive enzyme immunoassay systems. - Highlights: • Ab2β−Nb−AP has the potential to replace chemically-coupled probes. • Ab2β−Nb−AP is homogeneous enzyme-labelled antigen can be prepared reproducibly. • We developed a green and rapid one-step competitive enzyme immunoassay. • The sensitivity of one-step CLIA was 9-folds higher than two-step ELISA.

Convenient one-step synthesis of 5-carboxy-seminaphthofluoresceins

DEFF Research Database (Denmark)

Hammershøj, Peter; Thyhaug, Erling; Harris, Pernille

2017-01-01

The one-step synthesis and characterization of a series of regioisomerically pure 5-carboxy-seminaphthofluoresceins (5-carboxy-SNAFLs) is reported. The optical properties were determined in aqueous buffer at around biological pH, and highly pH sensitive, large Stokes-shift fluorophores with emiss......The one-step synthesis and characterization of a series of regioisomerically pure 5-carboxy-seminaphthofluoresceins (5-carboxy-SNAFLs) is reported. The optical properties were determined in aqueous buffer at around biological pH, and highly pH sensitive, large Stokes-shift fluorophores...

Vygotsky's Principle "One Step in Learning - One Hundred Steps In Development": From Idea To Practice

Directory of Open Access Journals (Sweden)

Zaretsky V.K.,

2016-12-01

Full Text Available The article reviews Lev Vygotsky’s published works to trace the evolution of his understanding of child development. The authors believe that his assumption that one step in learning may mean one hundred steps in development, is as important as the two other key postulates of the cultural-historical theory: the principle that learning precedes development and the concept of zone of proximal development. The authors provide a rationale for utilization of these assumptions in the practice of development-facilitating psychological and educational assistance. A mechanism of this learning-development relationship is hypothesized. The article outlines a multidimensional model of the zone of proximal development illustrating the above mechanism. This model is one of the conceptual tools of the Reflection and Activity Approach helping children overcome learning difficulties and promoting their development. Having given the account of how they proceeded “from the idea to the problem” and “from the idea to the mechanism”, the authors provide case studies showing how this mechanism allows working with learning difficulties to trigger simultaneous improvement in multiple developmental dimensions. The article reports on the experience of running special Summer Schools for children with learning difficulties, implementing the “Chess for General Development” Project, and assisting orphaned children with severe somatic conditions. A case study of a female college student displaying signs of the learned helplessness syndrome is presented. The authors infer that Vygotsky’s idea of a specific relationship between learning and development may be of fundamental theoretical and practical value, especially for working with children with special needs.

One-Step Leapfrog LOD-BOR-FDTD Algorithm with CPML Implementation

Directory of Open Access Journals (Sweden)

Yi-Gang Wang

2016-01-01

Full Text Available An unconditionally stable one-step leapfrog locally one-dimensional finite-difference time-domain (LOD-FDTD algorithm towards body of revolution (BOR is presented. The equations of the proposed algorithm are obtained by the algebraic manipulation of those used in the conventional LOD-BOR-FDTD algorithm. The equations for z-direction electric and magnetic fields in the proposed algorithm should be treated specially. The new algorithm obtains a higher computational efficiency while preserving the properties of the conventional LOD-BOR-FDTD algorithm. Moreover, the convolutional perfectly matched layer (CPML is introduced into the one-step leapfrog LOD-BOR-FDTD algorithm. The equation of the one-step leapfrog CPML is concise. Numerical results show that its reflection error is small. It can be concluded that the similar CPML scheme can also be easily applied to the one-step leapfrog LOD-FDTD algorithm in the Cartesian coordinate system.

One-step synthesis of bird cage-like ZnO and other controlled morphologies: Structural, growth mechanism and photocatalytic properties

International Nuclear Information System (INIS)

Yang, Shuo; Wang, Jian; Li, Xiuyan; Zhai, Hongju; Han, Donglai; Wei, Bing; Wang, Dandan; Yang, Jinghai

2014-01-01

Highlights: • ZnO nanocage arrays were synthesized by a one-step etching route. • ZnO nanocage exhibit higher photocatalytic activity than other samples. • The different photocatalytic activities of different samples were analyzed. • The formation mechanism of ZnO nanocages was proposed. - Abstract: ZnO nanocages and other nanostructures have been synthesized via a simple one-pot hydrothermal method with different reaction times. It is worth mentioning that this is a completely green method which does not require any other chemicals except that Zn foil served as Zn source in the experiment. X-ray diffraction (XRD), Scanning electron microscopy (SEM), transmission electron microscopy (TEM), photoluminescence (PL) and UV–Vis diffuse reflection spectra were used to characterize the crystallinity, morphology and optical property of ZnO structures. Growth mechanisms of ZnO were proposed based on these results. Furthermore, ZnO films with different morphologies and crystal growth habits exhibited different activities to rhodamine B degradation. The influence of the reaction time on the morphology of ZnO films and the effect of the morphologies on the photocatalytic activity are discussed

One-step implementation of the Toffoli gate via quantum Zeno dynamics

International Nuclear Information System (INIS)

Shao Xiaoqiang; Wang Hongfu; Chen Li; Zhang Shou; Yeon, Kyu-Hwang

2009-01-01

Based on the quantum Zeno dynamics, we present a scheme for one-step implementation of a Toffoli gate via manipulating three rf superconducting quantum interference device (SQUID) qubits to resonantly interact with a superconducting cavity. The effects of decoherence such as spontaneous emission and the loss of cavity are also considered.

A one-step method to fabricate PLLA scaffolds with deposition of bioactive hydroxyapatite and collagen using ice-based microporogens

Science.gov (United States)

Li, Jiashen; Chen, Yun; Mak, Arthur F.T.; Tuan, Rocky S.; Li, Lin; Li, Yi

2010-01-01

Porous poly(L-lactic acid) (PLLA) scaffolds with bioactive coatings were prepared by a novel one-step method. In this process, ice-based microporogens containing bioactive molecules, such as hydroxyapatite (HA) and collagen, served as both porogens to form the porous structure and vehicles to transfer the bioactive molecules to the inside of PLLA scaffolds in a single step. Based on scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) analysis, the bioactive components were found to be transferred successfully from the porogens to PLLA scaffolds evenly. Osteoblast cells were used to evaluate the cellular behaviors of the composite scaffolds. After 8 days culturing, MTT assay and alkaline phosphatase (ALP) activity results suggested that HA/collagen could improve the interactions between osteoblast cells and the polymeric scaffold. PMID:20004261

Green's functions of one-dimensional quasicrystal bi-material with piezoelectric effect

Energy Technology Data Exchange (ETDEWEB)

Zhang, Liangliang [College of Engineering, China Agricultural University, Beijing 100083 (China); Sinomatech Wind Power Blade Co., Ltd, Beijing 100092 (China); Wu, Di [College of Engineering, China Agricultural University, Beijing 100083 (China); Xu, Wenshuai [College of Science, China Agricultural University, Beijing 100083 (China); Yang, Lianzhi [Civil and Environmental Engineering School, University of Science and Technology Beijing, Beijing 100083 (China); Ricoeur, Andreas; Wang, Zhibin [Institute of Mechanics, University of Kassel, 34125 Kassel (Germany); Gao, Yang, E-mail: gaoyangg@gmail.com [College of Science, China Agricultural University, Beijing 100083 (China)

2016-09-16

Based on the Stroh formalism of one-dimensional quasicrystals with piezoelectric effect, the problems of an infinite plane composed of two different quasicrystal half-planes are taken into account. The solutions of the internal and interfacial Green's functions of quasicrystal bi-material are obtained. Moreover, numerical examples are analyzed for a quasicrystal bi-material subjected to line forces or line dislocations, showing the contour maps of the coupled fields. The impacts of changing material constants on the coupled field components are investigated. - Highlights: • Green's functions of 1D piezoelectric quasicrystal bi-material are studied. • The coupled fields subjected to line forces or line dislocations are obtained. • Mechanical behavior under the effect of different material constants is researched.

One-step process of hydrothermal and alkaline treatment of wheat straw for improving the enzymatic saccharification.

Science.gov (United States)

Sun, Shaolong; Zhang, Lidan; Liu, Fang; Fan, Xiaolin; Sun, Run-Cang

2018-01-01

To increase the production of bioethanol, a two-step process based on hydrothermal and dilute alkaline treatment was applied to reduce the natural resistance of biomass. However, the process required a large amount of water and a long operation time due to the solid/liquid separation before the alkaline treatment, which led to decrease the pure economic profit for production of bioethanol. Therefore, four one-step processes based on order of hydrothermal and alkaline treatment have been developed to enhance concentration of glucose of wheat straw by enzymatic saccharification. The aim of the present study was to systematically evaluated effect for different one-step processes by analyzing the physicochemical properties (composition, structural change, crystallinity, surface morphology, and BET surface area) and enzymatic saccharification of the treated substrates. In this study, hemicelluloses and lignins were removed from wheat straw and the morphologic structures were destroyed to various extents during the four one-step processes, which were favorable for cellulase absorption on cellulose. A positive correlation was also observed between the crystallinity and enzymatic saccharification rate of the substrate under the conditions given. The surface area of the substrate was positively related to the concentration of glucose in this study. As compared to the control (3.0 g/L) and treated substrates (11.2-14.6 g/L) obtained by the other three one-step processes, the substrate treated by one-step process based on successively hydrothermal and alkaline treatment had a maximum glucose concentration of 18.6 g/L, which was due to the high cellulose concentration and surface area for the substrate, accompanying with removal of large amounts of lignins and hemicelluloses. The present study demonstrated that the order of hydrothermal and alkaline treatment had significant effects on the physicochemical properties and enzymatic saccharification of wheat straw. The one-step

The sensitivity and efficacy method of PIK3CA exon 9 E545A as a high diagnostic accuracy in breast cancer

Directory of Open Access Journals (Sweden)

Desriani

2018-06-01

Full Text Available The phosphatidylinositol 3-kinases (PIK3s are lipid kinases. Mutation in the exon 9 and exon 20 determined as a predictive factor in anti-HER-2 therapy. In some countries, such as Singapore, China, and Peru, PIK3CA exon 9 E545A was reported to produce the highest rate of mutation. In this research, we developed and optimized PIK3CA exon 9 E545A detection methods with intercalating dye SYBR Green I based on the Tm Shift approach by using prepared recombinant plasmid pGEMT-easy PIK3CA exon 9 and PIK3CA exon 9 E545A. Recombinant plasmid was used due to the limited number of samples. Methods: Recombinant plasmid was prepared based on manufactured procedures, and this process was then followed by Tm prediction with Poland software, Tm Shift SYBR Green I development, and its characterization (reproducibility, repeatability, sensitivity, qPCR efficiency, and qPCR amplification, respectively. Result: A method for PIK3CA E545A detection based on TM shift SYBR Green I has been successfully developed. The melting temperature for PIK3CA exon 9 was 78.1 ± 0.1 °C, while that for PIK3CA exon E545A was 80.20 °C. The Tm of mutant was the same as that predicted using Polland Software. The reproducibility of the methods was high, with the coefficient values for inter and intra assays were below 10% with a high sensitivity at 1%, while R2 0.99 and PCR efficiency was 97.75%. Conclusion: The results presented here demonstrate that the PIK3CA exon 9 E545A detection method has a good sensitivity and efficacy assay, which proves that the method has a high diagnostic accuracy in breast cancer. Keywords: SYBR Green I, PIK3CA E545A, Breast cancer, Real time PCR, Recombinant plasmid

Next steps for a Green Economy Working Group in Kazakhstan. Notes from the Astana Green Economy Dialogue, 24-26 November 2011

Energy Technology Data Exchange (ETDEWEB)

Ospanova, Saule; Wilson, Emma; Bass, Steve

2013-01-15

In the Republic of Kazakhstan, national concerns over today’s economic, social and environmental challenges have translated into sustainable development policy and initiatives such as the Astana Green Bridge Initiative. The Government of Kazakhstan has developed the Green Bridge Partnership Programme (GBPP), with the support of international organizations, for possible adoption at the Rio+20 World Sustainable Development Conference in June 2012. This programme offers opportunities for 'greening' the economy, with a focus on aspirations for regional and international technology cooperation and finance. it also offers potential for enhancing public participation in decision-making, and harmonising policies and practices across European, Asian and Pacific regions. A range of other initiatives are also ongoing within Kazakhstan, and it is important for those promoting these initiatives to join forces and engage in dialogue. The Astana Green Economy Dialogue, held from 24th to 26th November 2011, organised by IIED and the Kazakhstan Ministry for the Environment and supported by the UK Foreign and Commonwealth Office (FCO) and the Organisation for Security and Co-operation in Europe (OSCE), brought together a range of stakeholders from government, civil society and industry to discuss the notion of the green economy and how it can be applied and developed in Kazakhstan. The dialogue had a particular focus on the energy sector given its relevance. Oil-producing states face a global challenge to play their part in establishing economic systems that reduce climate change and other environmental burdens, and to produce higher societal value from limited natural resources. This short report summarises the key observations and ideas discussed at the workshop, with recommendations for next steps and follow up. It is meant to provide a record of the discussions that took place at this dialogue and provides a foundation for further work.

One step versus two step approach for gestational diabetes screening: systematic review and meta-analysis of the randomized trials.

Science.gov (United States)

Saccone, Gabriele; Caissutti, Claudia; Khalifeh, Adeeb; Meltzer, Sara; Scifres, Christina; Simhan, Hyagriv N; Kelekci, Sefa; Sevket, Osman; Berghella, Vincenzo

2017-12-03

To compare both the prevalence of gestational diabetes mellitus (GDM) as well as maternal and neonatal outcomes by either the one-step or the two-step approaches. Electronic databases were searched from their inception until June 2017. We included all randomized controlled trials (RCTs) comparing the one-step with the two-step approaches for the screening and diagnosis of GDM. The primary outcome was the incidence of GDM. Three RCTs (n = 2333 participants) were included in the meta-analysis. 910 were randomized to the one step approach (75 g, 2 hrs), and 1423 to the two step approach. No significant difference in the incidence of GDM was found comparing the one step versus the two step approaches (8.4 versus 4.3%; relative risk (RR) 1.64, 95%CI 0.77-3.48). Women screened with the one step approach had a significantly lower risk of preterm birth (PTB) (3.7 versus 7.6%; RR 0.49, 95%CI 0.27-0.88), cesarean delivery (16.3 versus 22.0%; RR 0.74, 95%CI 0.56-0.99), macrosomia (2.9 versus 6.9%; RR 0.43, 95%CI 0.22-0.82), neonatal hypoglycemia (1.7 versus 4.5%; RR 0.38, 95%CI 0.16-0.90), and admission to neonatal intensive care unit (NICU) (4.4 versus 9.0%; RR 0.49, 95%CI 0.29-0.84), compared to those randomized to screening with the two step approach. The one and the two step approaches were not associated with a significant difference in the incidence of GDM. However, the one step approach was associated with better maternal and perinatal outcomes.

One-step electrochemically expanded graphite foil for flexible all-solid supercapacitor with high rate performance

International Nuclear Information System (INIS)

Li, Han-Yu; Yu, Yao; Liu, Lang; Liu, Lin; Wu, Yue

2017-01-01

Flexible solid-state supercapacitors (SSCs) as a candidate for energy storage source, have been attracting intensive attention. Graphene-based materials for SSCs have been widely studied. However, most reported preparation methods for graphene-based materials are energy-consuming, time-consuming and environmentally hazardous, what’s more, the assembling of SSCs need additives, such as current collectors, flexible substrates. So, it is necessary to develop simpler and greener attempts to achieve high-performance, cost-effective, substrates/additives-free and flexible electrodes for SSC devices. Herein, we reported a green and facile one-step process of electrochemical oxidation and expansion in salt solution to activate graphite foil (GF) for fabricating expanded graphite foil (EGF). The EGF electrode with unique structure and high conductivity showed high supercapacitor performance of 65 mF cm −2 , remarkable rate-capability maintaining at a level of 80% even at a current density of 20 mA cm −2 and excellent cycling stability with ∼95% capacitance remaining after 10000 cycles at a current density of 20 mA cm −2 . Moreover, a symmetric flexible all-solid supercapacitor (SSC) device was integrated using EGFs without any current collectors and additives. The flexible EGF-based device showed a high capacitance capacity of 30.5 mF cm −2 , excellent rate performance and good cycle stability which make it holds promise for applications in flexible, portable and wearable electronic devices.

One-step synthesis of hybrid nanocrystals with rational tuning of the morphology.

Science.gov (United States)

Sang, Wei; Zheng, Tingting; Wang, Youcheng; Li, Xu; Zhao, Xu; Zeng, Jie; Hou, J G

2014-11-12

Metal-sulfide hybrid nanocrystals (HNCs) have been of great interest for their distinguished interfacial effect, which gives rise to unique catalytic properties. However, most of the reported metal-sulfide HNCs were synthesized via two-step approaches and few were fabricated based on the one-step strategies. Herein, we report a facile one-pot synthesis of CuPt-Cu2S, Pt-Cu2S HNCs, and CuPt nanocubes by simply changing the Pt precursor types. 1-Hexadecanethiol (HDT) was employed in this system to mediate the reduction of metal precursors and also as capping agent and sulfur source. Moreover, CuPd-Cu2S and Au-Cu2S HNCs were successfully prepared by using this one-step method. The catalytic properties of the obtained three nanocrystals were investigated in hydrogenation of cinnamaldehyde. Results show that CuPt-Cu2S HNCs exhibited the highest conversion rate and the highest selectivity toward hydrocinnamaldehyde while 3-phenyl-1-propanol was the only product over Pt-Cu2S HNCs.

One-step synthesis of gold-polyaniline core-shell particles

International Nuclear Information System (INIS)

Wang Zhijuan; Yuan Junhua; Han Dongxue; Niu Li; Ivaska, Ari

2007-01-01

A one-step method has been developed for synthesizing gold-polyaniline (Au-PANI) core-shell particles by using chlorauric acid (HAuCl 4 ) to oxidize aniline in the presence of acetic acid and Tween 40 at room temperature. SEM images indicated that the resulting core-shell particles were composed of submicrometre-scale Au particles and PANI shells with an average thickness of 25 nm. Furthermore, a possible mechanism concerning the growth of Au-PANI particles was also proposed based on the results of control experiments

Comparison of multiple DNA dyes for real-time PCR: effects of dye concentration and sequence composition on DNA amplification and melting temperature

DEFF Research Database (Denmark)

Guðnason, Haukur; Dufva, Hans Martin; Bang, Dang Duong

2007-01-01

investigate 15 different intercalating DNA dyes for their inhibitory effects on PCR, effects on DNA melting temperature and possible preferential binding to GC-rich sequences. Our results demonstrated that in contrast to the results of SYBR Green I, two intercalating dyes SYTO-13 and SYTO-82 do not inhibit......The importance of real-time polymerase chain reaction (PCR) has increased steadily in clinical applications over the last decade. Many applications utilize SYBR Green I dye to follow the accumulation of amplicons in real time. SYBR Green I has, however, a number of limitations that include...... the inhibition of PCR, preferential binding to GC-rich sequences and effects on melting curve analysis. Although a few alternative dyes without some of these limitations have been recently proposed, no large-scale investigation into the properties of intercalating dyes has been performed. In this study, we...

One step linear reconstruction method for continuous wave diffuse optical tomography

Science.gov (United States)

Ukhrowiyah, N.; Yasin, M.

2017-09-01

The method one step linear reconstruction method for continuous wave diffuse optical tomography is proposed and demonstrated for polyvinyl chloride based material and breast phantom. Approximation which used in this method is selecting regulation coefficient and evaluating the difference between two states that corresponding to the data acquired without and with a change in optical properties. This method is used to recovery of optical parameters from measured boundary data of light propagation in the object. The research is demonstrated by simulation and experimental data. Numerical object is used to produce simulation data. Chloride based material and breast phantom sample is used to produce experimental data. Comparisons of results between experiment and simulation data are conducted to validate the proposed method. The results of the reconstruction image which is produced by the one step linear reconstruction method show that the image reconstruction almost same as the original object. This approach provides a means of imaging that is sensitive to changes in optical properties, which may be particularly useful for functional imaging used continuous wave diffuse optical tomography of early diagnosis of breast cancer.

One step geometrical calibration method for optical coherence tomography

International Nuclear Information System (INIS)

Díaz, Jesús Díaz; Ortmaier, Tobias; Stritzel, Jenny; Rahlves, Maik; Reithmeier, Eduard; Roth, Bernhard; Majdani, Omid

2016-01-01

We present a novel one-step calibration methodology for geometrical distortion correction for optical coherence tomography (OCT). A calibration standard especially designed for OCT is introduced, which consists of an array of inverse pyramidal structures. The use of multiple landmarks situated on four different height levels on the pyramids allow performing a 3D geometrical calibration. The calibration procedure itself is based on a parametric model of the OCT beam propagation. It is validated by experimental results and enables the reduction of systematic errors by more than one order of magnitude. In future, our results can improve OCT image reconstruction and interpretation for medical applications such as real time monitoring of surgery. (paper)

A rapid, one step molecular identification of Trichoderma citrinoviride and Trichoderma reesei.

Science.gov (United States)

Saroj, Dina B; Dengeti, Shrinivas N; Aher, Supriya; Gupta, Anil K

2015-06-01

Trichoderma species are widely used as production hosts for industrial enzymes. Identification of Trichoderma species requires a complex molecular biology based identification involving amplification and sequencing of multiple genes. Industrial laboratories are required to run identification tests repeatedly in cell banking procedures and also to prove absence of production host in the product. Such demands can be fulfilled by a brief method which enables confirmation of strain identity. This communication describes one step identification method for two common Trichoderma species; T. citrinoviride and T. reesei, based on identification of polymorphic region in the nucleotide sequence of translation elongation factor 1 alpha. A unique forward primer and common reverse primer resulted in 153 and 139 bp amplicon for T. citrinoviride and T. reesei, respectively. Simplification was further introduced by using mycelium as template for PCR amplification. Method described in this communication allows rapid, one step identification of two Trichoderma species.

A proposal for a green supply chain strategy

Directory of Open Access Journals (Sweden)

Carola Pinto Taborga

2018-05-01

Findings: The paper identifies some specific steps for developing a Green Supply Chain Strategy. The case study developed, demonstrates the importance of following a proper methodology based on a set of steps, it also demonstrates that some alternatives focus on improving the supply chain, such as the facilities location, can also improve the key performance indicator related with carbon emission.  Originality/value: The study provides guidance for manufacturing companies in implementing their Green Supply Chain Strategy.

One-Step Dynamic Classifier Ensemble Model for Customer Value Segmentation with Missing Values

Directory of Open Access Journals (Sweden)

Jin Xiao

2014-01-01

Full Text Available Scientific customer value segmentation (CVS is the base of efficient customer relationship management, and customer credit scoring, fraud detection, and churn prediction all belong to CVS. In real CVS, the customer data usually include lots of missing values, which may affect the performance of CVS model greatly. This study proposes a one-step dynamic classifier ensemble model for missing values (ODCEM model. On the one hand, ODCEM integrates the preprocess of missing values and the classification modeling into one step; on the other hand, it utilizes multiple classifiers ensemble technology in constructing the classification models. The empirical results in credit scoring dataset “German” from UCI and the real customer churn prediction dataset “China churn” show that the ODCEM outperforms four commonly used “two-step” models and the ensemble based model LMF and can provide better decision support for market managers.

Multi-step wrought processing of TiAl-based alloys

International Nuclear Information System (INIS)

Fuchs, G.E.

1997-04-01

Wrought processing will likely be needed for fabrication of a variety of TiAl-based alloy structural components. Laboratory and development work has usually relied on one-step forging to produce test material. Attempts to scale-up TiAl-based alloy processing has indicated that multi-step wrought processing is necessary. The purpose of this study was to examine potential multi-step processing routes, such as two-step isothermal forging and extrusion + isothermal forging. The effects of processing (I/M versus P/M), intermediate recrystallization heat treatments and processing route on the tensile and creep properties of Ti-48Al-2Nb-2Cr alloys were examined. The results of the testing were then compared to samples from the same heats of materials processed by one-step routes. Finally, by evaluating the effect of processing on microstructure and properties, optimized and potentially lower cost processing routes could be identified

Duplex real-time PCR assay using SYBR Green to detect and quantify Malayan box turtle (Cuora amboinensis) materials in meatballs, burgers, frankfurters and traditional Chinese herbal jelly powder.

Science.gov (United States)

Asing; Ali, Eaqub; Hamid, Sharifah Bee Abd; Hossain, Motalib; Ahamad, Mohammad Nasir Uddin; Hossain, S M Azad; Naquiah, Nina; Zaidul, I S M

2016-11-01

The Malayan box turtle (Cuora amboinensis) (MBT) is a vulnerable and protected species widely used in exotic foods and traditional medicines. Currently available polymerase chain reaction (PCR) assays to identify MBT lack automation and involve long targets which break down in processed or denatured tissue. This SYBR Green duplex real-time PCR assay has addressed this research gap for the first time through the combination of 120- and 141-bp targets from MBT and eukaryotes for the quantitative detection of MBT DNA in food chain and herbal medicinal preparations. This authentication ensures better security through automation, internal control and short targets that were stable under the processing treatments of foods and medicines. A melting curve clearly demonstrated two peaks at 74.63 ± 0.22 and 78.40 ± 0.31°C for the MBT and eukaryotic products, respectively, under pure, admixed and commercial food matrices. Analysis of 125 reference samples reflected a target recovery of 93.25-153.00%, PCR efficiency of 99-100% and limit of detection of 0.001% under various matrices. The quantification limits were 0.00001, 0.00170 ± 0.00012, 0.00228 ± 0.00029, 0.00198 ± 0.00036 and 0.00191 ± 0.00043 ng DNA for the pure meat, binary mixtures, meatball, burger and frankfurter products, respectively. The assay was used to screen 100 commercial samples of traditional Chinese herbal jelly powder from eight different brands; 22% of them were found to be MBT-positive (5.37 ± 0.50-7.00 ± 0.34% w/w), which was reflected through the Ct values (26.37 ± 0.32-28.90 ± 0.42) and melting curves (74.63-78.65 ± 0.22°C) of the amplified MBT target (120 bp), confirming the speculation that MBT materials are widely used in Chinese herbal desserts, exotic dishes consumed with the hope of prolonging life and youth.

A one-step miniprep for the isolation of plasmid DNA and lambda phage particles.

Directory of Open Access Journals (Sweden)

George Lezin

Full Text Available Plasmid DNA minipreps are fundamental techniques in molecular biology. Current plasmid DNA minipreps use alkali and the anionic detergent SDS in a three-solution format. In addition, alkali minipreps usually require additional column-based purification steps and cannot isolate other extra-chromosomal elements, such as bacteriophages. Non-ionic detergents (NIDs have been used occasionally as components of multiple-solution plasmid DNA minipreps, but a one-step approach has not been developed. Here, we have established a one-tube, one-solution NID plasmid DNA miniprep, and we show that this approach also isolates bacteriophage lambda particles. NID minipreps are more time-efficient than alkali minipreps, and NID plasmid DNA performs better than alkali DNA in many downstream applications. In fact, NID crude lysate DNA is sufficiently pure to be used in digestion and sequencing reactions. Microscopic analysis showed that the NID procedure fragments E. coli cells into small protoplast-like components, which may, at least in part, explain the effectiveness of this approach. This work demonstrates that one-step NID minipreps are a robust method to generate high quality plasmid DNA, and NID approaches can also isolate bacteriophage lambda particles, outperforming current standard alkali-based minipreps.

Colloidal Gold--Collagen Protein Core--Shell Nanoconjugate: One-Step Biomimetic Synthesis, Layer-by-Layer Assembled Film, and Controlled Cell Growth.

Science.gov (United States)

Xing, Ruirui; Jiao, Tifeng; Yan, Linyin; Ma, Guanghui; Liu, Lei; Dai, Luru; Li, Junbai; Möhwald, Helmuth; Yan, Xuehai

2015-11-11

The biogenic synthesis of biomolecule-gold nanoconjugates is of key importance for a broad range of biomedical applications. In this work, a one-step, green, and condition-gentle strategy is presented to synthesize stable colloidal gold-collagen core-shell nanoconjugates in an aqueous solution at room temperature, without use of any reducing agents and stabilizing agents. It is discovered that electrostatic binding between gold ions and collagen proteins and concomitant in situ reduction by hydroxyproline residues are critically responsible for the formation of the core-shell nanoconjugates. The film formed by layer-by-layer assembly of such colloidal gold-collagen nanoconjugates can notably improve the mechanical properties and promote cell adhesion, growth, and differentiation. Thus, the colloidal gold-collagen nanoconjugates synthesized by such a straightforward and clean manner, analogous to a biomineralization pathway, provide new alternatives for developing biologically based hybrid biomaterials toward a range of therapeutic and diagnostic applications.

Green Mines green energy : establishing productive land on mine tailings

Energy Technology Data Exchange (ETDEWEB)

Tisch, B.; Zinck, J.; Vigneault, B. [Natural Resources Canada, Ottawa, ON (Canada). CANMET Mining and Mineral Sciences Laboratories

2009-02-15

The Green Mines green energy research project was initiated by the CANMET Mining and Mineral Sciences Laboratories of Natural Resources Canada. The objective of the initiative was to demonstrate that organic residuals could be used to remediate mine tailings and establish agriculturally productive land where energy crops such as corn, canola, soy, switchgrass and other species could be grown and harvested specifically as feedstock for the production of green fuels. This paper discussed the scope and progress to date of the Green Mines green energy project. This included discussion about a column leaching study and about effluent treatability and toxicity. Neutralization test results and the results of field trials were presented. The paper concluded with a discussion of next steps. An advisory committee has been established to review annual progress and establish research directions. Overall, preliminary results from the column study suggest that sulphate reduction at the tailings-biosolids interface is occurring, although steady state has not yet been reached after more than one year of testing. 1 tab., 3 figs.

Green Mines green energy : establishing productive land on mine tailings

International Nuclear Information System (INIS)

Tisch, B.; Zinck, J.; Vigneault, B.

2009-01-01

The Green Mines green energy research project was initiated by the CANMET Mining and Mineral Sciences Laboratories of Natural Resources Canada. The objective of the initiative was to demonstrate that organic residuals could be used to remediate mine tailings and establish agriculturally productive land where energy crops such as corn, canola, soy, switchgrass and other species could be grown and harvested specifically as feedstock for the production of green fuels. This paper discussed the scope and progress to date of the Green Mines green energy project. This included discussion about a column leaching study and about effluent treatability and toxicity. Neutralization test results and the results of field trials were presented. The paper concluded with a discussion of next steps. An advisory committee has been established to review annual progress and establish research directions. Overall, preliminary results from the column study suggest that sulphate reduction at the tailings-biosolids interface is occurring, although steady state has not yet been reached after more than one year of testing. 1 tab., 3 figs

Zinc hexacyanoferrate film as an effective protecting layer in two-step and one-step electropolymerization of pyrrole on zinc substrate

Energy Technology Data Exchange (ETDEWEB)

Pournaghi-Azar, M.H. [Electroanalytical Chemistry Laboratory, Faculty of Chemistry, University of Tabriz, Tabriz (Iran, Islamic Republic of)]. E-mail: pournaghiazar@tabrizu.ac.ir; Nahalparvari, H. [Electroanalytical Chemistry Laboratory, Faculty of Chemistry, University of Tabriz, Tabriz (Iran, Islamic Republic of)

2005-03-15

The two-step and one-step electrosynthesis processes of polypyrrole (PPy) films on the zinc substrate are described. The two-step process includes (i) the zinc surface pretreatment with hexacyanoferrate ion in the aqueous medium in order to form a zinc hexacyanoferrate (ZnHCF) film non-blocking passive layer on the surface and with the view to prevent its reactivity and (ii) electropolymerization of pyrrole on the ZnHCF vertical bar Zn-modified electrode in aqueous pyrrole solution. In this context, both the non-electrolytic and electrolytic procedures were adapted, and the effect of some experimental conditions such as supporting electrolyte, pH and temperature of the solution at the zinc surface pretreatment step as well as pyrrole concentration and electrochemical techniques at the polymerization step was investigated. By optimizing the experimental conditions in both steps, we have obtained a homogeneous and strongly adherent PPy films on the zinc substrate. The one-step process is based on the use of an aqueous medium containing Fe(CN){sub 6}{sup 4-} and pyrrole. The ferrocyanide ion passivates the substrate by formation of ZnHCF film during the electropolymerization process of pyrrole and therefore makes it possible to obtain strongly adherent PPy films, with controlled thickness, either by cyclic voltammetry or by electrolysis at constant current or constant potential without any previously treatment of the zinc electrode surface. The polypyrrole films deposited on the zinc electrode were characterized by cyclic voltammetry and scanning electron microscopic (SEM) measurement.

Zinc hexacyanoferrate film as an effective protecting layer in two-step and one-step electropolymerization of pyrrole on zinc substrate

International Nuclear Information System (INIS)

Pournaghi-Azar, M.H.; Nahalparvari, H.

2005-01-01

The two-step and one-step electrosynthesis processes of polypyrrole (PPy) films on the zinc substrate are described. The two-step process includes (i) the zinc surface pretreatment with hexacyanoferrate ion in the aqueous medium in order to form a zinc hexacyanoferrate (ZnHCF) film non-blocking passive layer on the surface and with the view to prevent its reactivity and (ii) electropolymerization of pyrrole on the ZnHCF vertical bar Zn-modified electrode in aqueous pyrrole solution. In this context, both the non-electrolytic and electrolytic procedures were adapted, and the effect of some experimental conditions such as supporting electrolyte, pH and temperature of the solution at the zinc surface pretreatment step as well as pyrrole concentration and electrochemical techniques at the polymerization step was investigated. By optimizing the experimental conditions in both steps, we have obtained a homogeneous and strongly adherent PPy films on the zinc substrate. The one-step process is based on the use of an aqueous medium containing Fe(CN) 6 4- and pyrrole. The ferrocyanide ion passivates the substrate by formation of ZnHCF film during the electropolymerization process of pyrrole and therefore makes it possible to obtain strongly adherent PPy films, with controlled thickness, either by cyclic voltammetry or by electrolysis at constant current or constant potential without any previously treatment of the zinc electrode surface. The polypyrrole films deposited on the zinc electrode were characterized by cyclic voltammetry and scanning electron microscopic (SEM) measurement

One step approach towards the green synthesis of silver decorated graphene nanocomposites for the degradation of organic dyes in water

Science.gov (United States)

Karthik, Gopakumar; Harith, A.; Nazrin Thazleema, N.; Vishal, Shaji; Jayan Jitha, S.; Saritha, Appukuttan

2018-02-01

Recently the decoration of graphene with metallic nanoparticles by a one pot reduction of graphene oxide (GO) coupled with the synthesis of metallic nanoparticles has gained momentum. Graphene and GO have been proved to exhibit excellent biocompatibility and high antibacterial activity and hence a vast possibility lies in the utilization of GO as an antibacterial reinforcement in biomaterials and exploration of the antiseptic properties as well as the cytotoxicity of GO-containing composites. Moreover GO decorated with metal / metal oxide paves way towards an inevitable role in water purification. The use of graphene oxide as the nano scale substrates for the development of nanocomposites with metal oxides is a novel idea to obtain a hybrid which would exhibit both the properties of GO as a enthralling paper-shape material and the quality of single nano-sized metal particles. The heavy metal ions and pollutants are considered as a major problem in environmental contamination. Hence detection of trace level pollutant has become a hot topic in the present research scenario. Modified graphene oxide nanocomposites prepared using a green approach has the capacity of absorbing pollutant material ions in high efficiency and selectivity. The green synthesized nanocomposites were characterized using FTIR and UV spectroscopy and the consequence of pH and concentration on the preparation of the nanocomposites was evaluated. The efficiency of these nanocomposites towards degradation of organic dyes like methylene blue has been evaluated.

One-step synthesis of Pt-reduced graphene oxide composites based on high-energy radiation technique

International Nuclear Information System (INIS)

Liu, Xuqiang; Jiang, Shubin; Huang, Wei; Song, Hongtao

2014-01-01

In this paper, we introduce a novel 60 Co-ray-irradiation-based one-step synthesis method of Pt-reduced graphene oxide composites (Pt-RGO) in acid aqueous solution. The compositional distribution of the particles in the samples was characterized by transmission electron microscopy. The structure and composition of the nanocomposite has been determined with a scanning electron microscope (SEM) equipped with an energy dispersion X-ray (EDS) analyzer. Surface enhanced Raman scattering (SERS) of graphene deposited by the Pt nanoparticles were investigated with the 514.5 nm excitation. It was found that small-sized and highly-dispersed Pt nanoparticles could easily grow on the RGO surface under acidic conditions. In addition, the obtained homogeneous dispersions exhibit long-term stability, which will facilitate the production of homogeneous composites. (orig.)

One-Step Protein Conjugation to Upconversion Nanoparticles.

Science.gov (United States)

Lu, Jie; Chen, Yinghui; Liu, Deming; Ren, Wei; Lu, Yiqing; Shi, Yu; Piper, James; Paulsen, Ian; Jin, Dayong

2015-10-20

The emerging upconversion nanoparticles offer a fascinating library of ultrasensitive luminescent probes for a range of biotechnology applications from biomarker discovery to single molecule tracking, early disease diagnosis, deep tissue imaging, and drug delivery and therapies. The effective bioconjugation of inorganic nanoparticles to the molecule-specific proteins, free of agglomeration, nonspecific binding, or biomolecule deactivation, is crucial for molecular recognition of target molecules or cells. The current available protocols require multiple steps which can lead to low probe stability, specificity, and reproducibility. Here we report a simple and rapid protein bioconjugation method based on a one-step ligand exchange using the DNAs as the linker. Our method benefits from the robust DNA-protein conjugates as well as from multiple ions binding capability. Protein can be preconjugated via an amino group at the 3' end of a synthetic DNA molecule, so that the 5' end phosphoric acid group and multiple phosphate oxygen atoms in the phosphodiester bonds are exposed to replace the oleic acid ligands on the surface of upconversion nanoparticles due to their stronger chelating capability to lanthanides. We demonstrated that our method can efficiently pull out the upconversion nanoparticles from organic solvent into an aqueous phase. The upconversion nanoparticles then become hydrophilic, stable, and specific biomolecules recognition. This allows us to successfully functionalize the upconversion nanoparticles with horseradish peroxidise (HRP) for catalytic colorimetric assay and for streptavidin (SA)-biotin immunoassays.

Are women positive for the One Step but negative for the Two Step screening tests for gestational diabetes at higher risk for adverse outcomes?

Science.gov (United States)

Caissutti, Claudia; Khalifeh, Adeeb; Saccone, Gabriele; Berghella, Vincenzo

2018-02-01

The aim of this study was to evaluate if women meeting criteria for gestational diabetes mellitus (GDM) by the One Step test as per International Association of the Diabetes and Pregnancy Study Groups (IADPSG) criteria but not by other less strict criteria have adverse pregnancy outcomes compared with GDM-negative controls. The primary outcome was the incidence of macrosomia, defined as birthweight > 4000 g. Electronic databases were searched from their inception until May 2017. All studies identifying pregnant women negative at the Two Step test, but positive at the One Step test for IADPSG criteria were included. We excluded studies that randomized women to the One Step vs. the Two Step tests; studies that compared different criteria within the same screening method; randomized studies comparing treatments for GDM; and studies comparing incidence of GDM in women doing the One Step test vs. the Two Step test. Eight retrospective cohort studies, including 29 983 women, were included. Five study groups and four control groups were identified. The heterogeneity between the studies was high. Gestational hypertension, preeclampsia and large for gestational age, as well as in some analyses cesarean delivery, macrosomia and preterm birth, were significantly more frequent, and small for gestational age in some analyses significantly less frequent, in women GDM-positive by the One Step, but not the Two Step. Women meeting criteria for GDM by IADPSG criteria but not by other less strict criteria have an increased risk of adverse pregnancy outcomes such as gestational hypertension, preeclampsia and large for gestational age, compared with GDM-negative controls. Based on these findings, and evidence from other studies that treatment decreases these adverse outcomes, we suggest screening for GDM using the One Step IADPSG criteria. © 2017 Nordic Federation of Societies of Obstetrics and Gynecology.

GEOGRAPHIC INFORMATION SYSTEM-BASED MODELING AND ANALYSIS FOR SITE SELECTION OF GREEN MUSSEL, Perna viridis, MARICULTURE IN LADA BAY, PANDEGLANG, BANTEN PROVINCE

Directory of Open Access Journals (Sweden)

I Nyoman Radiarta

2011-06-01

Full Text Available Green mussel is one of important species cultured in Lada Bay, Pandeglang. To provide a necessary guidance regarding green mussel mariculture development, finding suitable site is an important step. This study was conducted to identify suitable site for green mussel mariculture development using geographic information system (GIS based models. Seven important parameters were grouped into two submodels, namely environmental (water temperature, salinity, suspended solid, dissolve oxygen, and bathymetry and infrastructural (distance to settlement and pond aquaculture. A constraint data was used to exclude the area from suitability maps that cannot be allowed to develop green mussel mariculture, including area of floating net fishing activity and area near electricity station. Analyses of factors and constraints indicated that about 31% of potential area with bottom depth less than 25 m had the most suitable area. This area was shown to have an ideal condition for green mussel mariculture in this study region. This study shows that GIS model is a powerful tool for site selection decision making. The tool can be a valuable tool in solving problems in local, regional, and/or continent areas.

One-step preparation of long-term stable and flexible CsPbBr3 perovskite quantum dots/ethylene vinyl acetate copolymer composite films for white LEDs.

Science.gov (United States)

Li, Yang; Lv, Ying; Guo, Ziquan; Dong, Liubing; Zheng, Jianghui; Chai, Chufen; Chen, Nan; Lu, Yijun; Chen, Chao

2018-04-19

CsPbBr3 perovskite quantum dots (PQDs)/ethylene vinyl acetate (EVA) composite films were prepared via a one-step method, based on that both supersaturated recrystallization of CsPbBr3 PQDs and dissolution of EVA were realized in toluene. The prepared films display outstanding green emitting performance with high color purity of 92% and photoluminescence quantum yield of 40.5% at appropriate CsPbBr3 PQD loading. They possess long-term stable luminescent properties in the air and in water, benefiting from the effective protection of CsPbBr3 PQDs by EVA matrix. Besides, the prepared CsPbBr3 PQDs/EVA films are flexible enough to be repeatedly bent for 1000 cycles while keeping unchanged photoluminescence intensity. Optical properties of the CsPbBr3 PQDs/EVA films in white LEDs were also studied by experiments and theoretical simulation. Overall, facile preparation process, good long-term stability and high flexibility allow our green-emitting CsPbBr3 PQDs/EVA films to be applied in lighting applications and flexible displays.

Relative sensitivity of conventional and real-time PCR assays for detection of SFG Rickettsia in blood and tissue samples from laboratory animals.

Directory of Open Access Journals (Sweden)

Galina E Zemtsova

Full Text Available Studies on the natural transmission cycles of zoonotic pathogens and the reservoir competence of vertebrate hosts require methods for reliable diagnosis of infection in wild and laboratory animals. Several PCR-based applications have been developed for detection of infections caused by Spotted Fever group Rickettsia spp. in a variety of animal tissues. These assays are being widely used by researchers, but they differ in their sensitivity and reliability. We compared the sensitivity of five previously published conventional PCR assays and one SYBR green-based real-time PCR assay for the detection of rickettsial DNA in blood and tissue samples from Rickettsia- infected laboratory animals (n = 87. The real-time PCR, which detected rickettsial DNA in 37.9% of samples, was the most sensitive. The next best were the semi-nested ompA assay and rpoB conventional PCR, which detected as positive 18.4% and 14.9% samples respectively. Conventional assays targeting ompB, gltA and hrtA genes have been the least sensitive. Therefore, we recommend the SYBR green-based real-time PCR as a tool for the detection of rickettsial DNA in animal samples due to its higher sensitivity when compared to more traditional assays.

House passes energy bill with one-step plant licensing

International Nuclear Information System (INIS)

Anon.

1992-01-01

The US House of Representatives which has traditionally been wary of measures that would allow nuclear power to expand, came down strongly on the side of nuclear when it approved a much-amended omnibus energy bill on May 27 by a vote of 381 to 37. The key for the nuclear industry is the presence in the bill (H.R. 776) of language on one-step power plant licensing that was taken directly from the Senate energy bill (S. 2166) that passed in February. This means that when the House and Senate work out a compromise version of the legislation, one-step licensing is almost certain to be carried through--and become law once the final bill is signed by President George Bush, which is expected later this year. The House's endorsement of nuclear power--both as it exists now, and as it could be with the introduction of new plant designs and an end to the long hiatus in plant orders by utilities--went beyond one-step licensing. Debate on the House floor prior to Memorial Day totally transformed the nuclear-related part of the energy bill. H.R. 776 was reported to the floor by the Rules Committee with language by the Interior and Insular Affairs Committee that would have created a nominal one-step system, with a full evidentiary hearing prior to plant construction but also allowing an intervenor who later presents new information on the plant to get another full evidentiary hearing after construction but before operation. This would effectively duplicate the two-step process that existed for all plants now in service, and which utilities no longer want to endure

LAMP assay for rapid diagnosis of cow DNA in goat milk and meat samples.

Science.gov (United States)

Deb, R; Sengar, G S; Singh, U; Kumar, S; Raja, T V; Alex, R; Alyethodi, R R; Prakash, B

2017-01-01

Animal species detection is one of the crucial steps for consumer's food analysis. In the present study we developed an in-house built loop-mediated isothermal amplification (LAMP) assay for rapid detection of adulterated cow DNA in goat milk/meat samples. The cow milk/tissue DNA in goat milk/meat samples were identified in the developed LAMP assay by either naked eye visualizing with SYBR Green I dyes or by detecting the typical ladder pattern on gel electrophoresis. This test can detect up to minimum 5% level of cow components admixed in goat milk/meat samples and can be completed within 1 h 40 min starting from DNA extraction from milk/meat samples and can be performed in a water bath. Developed LAMP methodology is simple; rapid and sensitive techniques that can detect adulterant like cow components in goat milk/meat are more accurate than other existing DNA based technologies.

Calculation of the MSD two-step process with the sudden approximation

Energy Technology Data Exchange (ETDEWEB)

Yoshida, Shiro [Tohoku Univ., Sendai (Japan). Dept. of Physics; Kawano, Toshihiko [Kyushu Univ., Advanced Energy Engineering Science, Kasuga, Fukuoka (Japan)

2000-03-01

A calculation of the two-step process with the sudden approximation is described. The Green's function which connects the one-step matrix element to the two-step one is represented in {gamma}-space to avoid the on-energy-shell approximation. Microscopically calculated two-step cross sections are averaged together with an appropriate level density to give a two-step cross section. The calculated cross sections are compared with the experimental data, however the calculation still contains several simplifications at this moment. (author)

Potato extract as reducing agent and stabiliser in a facile green one-step synthesis of ZnO nanoparticles

DEFF Research Database (Denmark)

Buazar, Foad; Bavi, M.; Kroushawi, Feisal

2016-01-01

A facile green recipe was developed to synthesise highly pure, safe and durable zinc oxide nanoparticles (ZnO Nps) using homemade starch-rich potato extract. The ZnO Nps were synthesised using zinc nitrate and potato extract, and the whole reaction is carried out for 30 min at 80 °C...

Green One-pot Synthesis of Novel Polysubstituted Pyrazole Derivatives as Potential Antimicrobial Agents.

Science.gov (United States)

Beyzaei, Hamid; Motraghi, Zahra; Aryan, Reza; Zahedi, Mohammad Mehdi; Samzadeh-Kermani, Alireza

2017-12-01

Various biological properties of natural and synthetic pyrazole derivatives such as anti-inflammatory, antimicrobial, neuroprotective, anticonvulsant, antidepressant and anticancer activities encouraged us to propose a new, fast, green and eco-friendly procedure for the preparation of some novel 5-amino-3-(aryl substituted)-1-(2,4-dinitrophenyl)-1H-pyrazole-4-carbonitriles. They were efficiently synthesized via one-pot two-step process reaction of malononitrile, 2,4-dinitrophenylhydrazine and different benzaldehydes in deep eutectic solvent (DES) glycerol/potassium carbonate. The products yield and reaction times were considerably improved in the presence of applied DES. Antibacterial effects of all newly synthesized pyrazoles in comparison with several common antibiotics were evaluated against a variety of Gram-positive and Gram-negative pathogenic bacteria. In addition to, their inhibitory activities on three fungi were compared to some current antifungal agents. The moderate to good antimicrobial potentials particularly against fungi were observed in the major heterocyclic compounds according to the IZD, MIC, MBC and MFC results.

One-step reduced kinetics for lean hydrogen-air deflagration

Energy Technology Data Exchange (ETDEWEB)

Fernandez-Galisteo, D.; Sanchez, A.L. [Area de Mecanica de Fluidos, Univ. Carlos III de Madrid, Leganes 28911 (Spain); Linan, A. [ETSI Aeronauticos, Pl. Cardenal Cisneros 3, Madrid 28040 (Spain); Williams, F.A. [Dept. of Mechanical and Aerospace Engineering, University of California San Diego, La Jolla, CA 92093-0411 (United States)

2009-05-15

A short mechanism consisting of seven elementary reactions, of which only three are reversible, is shown to provide good predictions of hydrogen-air lean-flame burning velocities. This mechanism is further simplified by noting that over a range of conditions of practical interest, near the lean flammability limit all reaction intermediaries have small concentrations in the important thin reaction zone that controls the hydrogen-air laminar burning velocity and therefore follow a steady state approximation, while the main species react according to the global irreversible reaction 2H{sub 2} + O{sub 2} {yields} 2H{sub 2}O. An explicit expression for the non-Arrhenius rate of this one-step overall reaction for hydrogen oxidation is derived from the seven-step detailed mechanism, for application near the flammability limit. The one-step results are used to calculate flammability limits and burning velocities of planar deflagrations. Furthermore, implications concerning radical profiles in the deflagration and reasons for the success of the approximations are clarified. It is also demonstrated that adding only two irreversible direct recombination steps to the seven-step mechanism accurately reproduces burning velocities of the full detailed mechanism for all equivalence ratios at normal atmospheric conditions and that an eight-step detailed mechanism, constructed from the seven-step mechanism by adding to it the fourth reversible shuffle reaction, improves predictions of O and OH profiles. The new reduced-chemistry descriptions can be useful for both analytical and computational studies of lean hydrogen-air flames, decreasing required computation times. (author)

Reconstruction of networks from one-step data by matching positions

Science.gov (United States)

Wu, Jianshe; Dang, Ni; Jiao, Yang

2018-05-01

It is a challenge in estimating the topology of a network from short time series data. In this paper, matching positions is developed to reconstruct the topology of a network from only one-step data. We consider a general network model of coupled agents, in which the phase transformation of each node is determined by its neighbors. From the phase transformation information from one step to the next, the connections of the tail vertices are reconstructed firstly by the matching positions. Removing the already reconstructed vertices, and repeatedly reconstructing the connections of tail vertices, the topology of the entire network is reconstructed. For sparse scale-free networks with more than ten thousands nodes, we almost obtain the actual topology using only the one-step data in simulations.

Green IT in Practice How One Company is Approaching the Greening of Its IT

CERN Document Server

Hird, Gary

2010-01-01

Green IT in Practice, Second edition provides guidance on how to implement a Green IT programme. It will help you to formulate a Green IT policy, curb demand for data storage capacity, and lower the electricity consumption of the datacentre.

PLACE-BASED GREEN BUILDING: INTEGRATING LOCAL ENVIRONMENTAL AND PLANNING ANALYSIS INTO GREEN BUILDING GUIDELINES

Science.gov (United States)

This project will develop a model for place-based green building guidelines based on an analysis of local environmental, social, and land use conditions. The ultimate goal of this project is to develop a methodology and model for placing green buildings within their local cont...

Effect of two-step and one-step surface conditioning of glass ceramic on adhesion strength of orthodontic bracket and effect of thermo-cycling on adhesion strength.

Science.gov (United States)

Asiry, Moshabab A; AlShahrani, Ibrahim; Alaqeel, Samer M; Durgesh, Bangalore H; Ramakrishnaiah, Ravikumar

2018-08-01

The adhesion strength of orthodontic brackets bonded to dental glass ceramics was evaluated after ceramic surface was treated with two-step and one-step surface conditioning systems, and subjecting to thermo-cycling. A total of forty specimens were fabricated from silica based glass ceramic (lithium disilicate) by duplicating the buccal surface of maxillary first premolar. The specimens were randomly assigned to two experimental groups (n = 20), group one specimens were treated with two-step surface conditioning system (IPS ceramic etching gel™ and Monobond plus™) and group two specimens were treated with one-step surface conditioning system (Monobond etch and prime™). The surface roughness of the specimens after treatment with two-step and one-step surface conditioning system was measured using non-contact surface profilometer. Ten randomly selected specimens from each group were subjected to thermo-cycling and the remaining ten served as baseline. The shear bond strength of the specimens was measured using universal material testing machine. The adhesive remnant index score was calculated, and the results of surface roughness and bond strength were tabulated and subjected to analysis of variance and post hoc tukey's test at a significance level of p step conditioning system had higher surface roughness and bond strength than one-step conditioning system. The majority of the specimens treated with both two-step and one-step conditioned specimens showed adhesive failure after subjecting thermo-cycling. Traditional two-step conditioning provides better bond strength. The clinical importance of the study is that, the silane promoted adhesion significantly reduces on exposure to thermo-cycling. Copyright © 2018 Elsevier Ltd. All rights reserved.

A One-Step-Ahead Smoothing-Based Joint Ensemble Kalman Filter for State-Parameter Estimation of Hydrological Models

KAUST Repository

El Gharamti, Mohamad

2015-11-26

The ensemble Kalman filter (EnKF) recursively integrates field data into simulation models to obtain a better characterization of the model’s state and parameters. These are generally estimated following a state-parameters joint augmentation strategy. In this study, we introduce a new smoothing-based joint EnKF scheme, in which we introduce a one-step-ahead smoothing of the state before updating the parameters. Numerical experiments are performed with a two-dimensional synthetic subsurface contaminant transport model. The improved performance of the proposed joint EnKF scheme compared to the standard joint EnKF compensates for the modest increase in the computational cost.

Ensemble Kalman filtering with one-step-ahead smoothing

KAUST Repository

Raboudi, Naila F.; Ait-El-Fquih, Boujemaa; Hoteit, Ibrahim

2018-01-01

error statistics. This limits their representativeness of the background error covariances and, thus, their performance. This work explores the efficiency of the one-step-ahead (OSA) smoothing formulation of the Bayesian filtering problem to enhance

Self-assembling organomodified Co/Al based layered double hydroxides (LDH) via one-step route

Institute of Scientific and Technical Information of China (English)

WANG De-yi; A.LEUTERITZ; U.WAGENKNECHT; G.HEINRICH

2009-01-01

The preparation of self-assembling organomodified Co/Al-layered double hydroxide (LDH) via one-step route was studied.A common surfactant,sodium dodecylbenzenesulfonate (DBS),was employed as an organic modifier.The behavior and structure of self-assembled intercalated organic Co/Al-LDH were investigated by FTIR,SEM,WAXS,element analysis and TGA.Based upon the WAXS results and calculation by Bragg equation,the interlayer distance (d value) for organic Co/Al-LDH is enlarged from 0.75 nm to 3.10 nm,showing that the self-assembling behavior has been carried out successfully.Considering the observation from SEM,the product shows the morphology of organic Co/Al-LDH of a layered structure.In addition,FTIR,element analysis and TGA analysis show that the modifier is intercalated into the gallery of the Co/Al-LDH.Since organic modification for nanofiller is deemed to be necessary before applying it into polymer,the successful preparation of organomodified Co/Al-LDH will be significantly beneficial to the preparation and investigation of novel polymer/LDH nanocomposite.

Teaching Green and Sustainable Chemistry: A Revised One-Semester Course Based on Inspirations and Challenges

Science.gov (United States)

Marteel-Parrish, Anne E.

2014-01-01

An elective course, "Toward the Greening of Our Minds": Green and Sustainable Chemistry, has been offered at Washington College since 2005. This new course without laboratory is designed for chemistry and biology majors and minors who have previously taken two semesters of general chemistry and organic chemistry. Due to the popularity of…

A trifunctional mesoporous silica-based, highly active catalyst for one-pot, three-step cascade reactions.

Science.gov (United States)

Biradar, Ankush V; Patil, Vijayshinha S; Chandra, Prakash; Doke, Dhananjay S; Asefa, Tewodros

2015-05-18

We report the synthesis of a trifunctional catalyst containing amine, sulphonic acid and Pd nanoparticle catalytic groups anchored on the pore walls of SBA-15. The catalyst efficiently catalyzes one-pot three-step cascade reactions comprising deacetylation, Henry reaction and hydrogenation, giving up to ∼100% conversion and 92% selectivity to the final product.

A permeation theory for single-file ion channels: one- and two-step models.

Science.gov (United States)

Nelson, Peter Hugo

2011-04-28

How many steps are required to model permeation through ion channels? This question is investigated by comparing one- and two-step models of permeation with experiment and MD simulation for the first time. In recent MD simulations, the observed permeation mechanism was identified as resembling a Hodgkin and Keynes knock-on mechanism with one voltage-dependent rate-determining step [Jensen et al., PNAS 107, 5833 (2010)]. These previously published simulation data are fitted to a one-step knock-on model that successfully explains the highly non-Ohmic current-voltage curve observed in the simulation. However, these predictions (and the simulations upon which they are based) are not representative of real channel behavior, which is typically Ohmic at low voltages. A two-step association/dissociation (A/D) model is then compared with experiment for the first time. This two-parameter model is shown to be remarkably consistent with previously published permeation experiments through the MaxiK potassium channel over a wide range of concentrations and positive voltages. The A/D model also provides a first-order explanation of permeation through the Shaker potassium channel, but it does not explain the asymmetry observed experimentally. To address this, a new asymmetric variant of the A/D model is developed using the present theoretical framework. It includes a third parameter that represents the value of the "permeation coordinate" (fractional electric potential energy) corresponding to the triply occupied state n of the channel. This asymmetric A/D model is fitted to published permeation data through the Shaker potassium channel at physiological concentrations, and it successfully predicts qualitative changes in the negative current-voltage data (including a transition to super-Ohmic behavior) based solely on a fit to positive-voltage data (that appear linear). The A/D model appears to be qualitatively consistent with a large group of published MD simulations, but no

One-step microlithography

Science.gov (United States)

Kahlen, Franz-Josef; Sankaranarayanan, Srikanth; Kar, Aravinda

1997-09-01

Subject of this investigation is a one-step rapid machining process to create miniaturized 3D parts, using the original sample material. An experimental setup where metal powder is fed to the laser beam-material interaction region has been built. The powder is melted and forms planar, 2D geometries as the substrate is moved under the laser beam in XY- direction. After completing the geometry in the plane, the substrate is displaced in Z-direction, and a new layer of material is placed on top of the just completed deposit. By continuous repetition of this process, 3D parts wee created. In particular, the impact of the focal spot size of the high power laser beam on the smallest achievable structures was investigated. At a translation speed of 51 mm/s a minimum material thickness of 590 micrometers was achieved. Also, it was shown that a small Z-displacement has a negligible influence on the continuity of the material deposition over this power range. A high power CO2 laser was used as energy source, the material powder under investigation was stainless steel SS304L. Helium was used as shield gas at a flow rate of 15 1/min. The incident CO2 laser beam power was varied between 300 W and 400 W, with the laser beam intensity distribute in a donut mode. The laser beam was focused to a focal diameter of 600 (Mu) m.

GREEN PACKAGING, GREEN PRODUCT, GREEN ADVERTISING, PERSEPSI, DAN MINAT BELI KONSUMEN

OpenAIRE

Imam Santoso; Rengganis Fitriani

2016-01-01

Environmental problems become one of the strategic issues in achieving global competitiveness. One of the issues is products that are made from environmental friendly materials or known as green product. Furthermore, in green products marketing, the company also uses green packaging and green advertising concept. This study aimed to analyze the effect of green packaging, green products, and green advertising on consumer perception and purchasing intention. The study was conducted in Ketawangg...

Presidential Green Chemistry Challenge: 1997 Greener Synthetic Pathways Award

Science.gov (United States)

Presidential Green Chemistry Challenge 1997 award winner, BHC Company, developed a highly atom-efficient method to make ibuprofen, a common painkiller, using three catalytic steps instead of six stoichiometric ones.

A new model in achieving Green Accounting at hotels in Bali

Science.gov (United States)

Astawa, I. P.; Ardina, C.; Yasa, I. M. S.; Parnata, I. K.

2018-01-01

The concept of green accounting becomes a debate in terms of its implementation in a company. The result of previous studies indicates that there are no standard model regarding its implementation to support performance. The research aims to create a different green accounting model to other models by using local cultural elements as the variables in building it. The research is conducted in two steps. The first step is designing the model based on theoretical studies by considering the main and supporting elements in building the concept of green accounting. The second step is conducting a model test at 60 five stars hotels started with data collection through questionnaire and followed by data processing using descriptive statistic. The result indicates that the hotels’ owner has implemented green accounting attributes and it supports previous studies. Another result, which is a new finding, shows that the presence of local culture, government regulation, and the awareness of hotels’ owner has important role in the development of green accounting concept. The results of the research give contribution to accounting science in terms of green reporting. The hotel management should adopt local culture in building the character of accountant hired in the accounting department.

Stability of one-step methods in transient nonlinear heat conduction

International Nuclear Information System (INIS)

Hughes, J.R.

1977-01-01

The purpose of the present work is to ascertain practical stability conditions for one-step methods commonly used in transient nonlinear heat conduction analyses. The class of problems considered is governed by a temporally continuous, spatially discrete system involving the capacity matrix C, conductivity matrix K, heat supply vector, temperature vector and time differenciation. In the linear case, in which K and C are constant, the stability behavior of one-step methods is well known. But in this paper the concepts of stability, appropriate to the nonlinear problem, are thoroughly discussed. They of course reduce to the usual stability criterion for the linear, constant coefficient case. However, for nonlinear problems there are differences and these ideas are of key importance in obtaining practical stability conditions. Of particular importance is a recent result which indicates that, in a sense, the trapezoidal and midpoint families are quivalent. Thus, stability results for one family may be translated into a result for the other. The main results obtained are summarized as follows. The stability behavior of the explicit Euler method in the nonlinear regime is analogous to that for linear problems. In particular, an a priori step size restriction may be determined for each time step. The precise time step restriction on implicit conditionally stable members of the trapezoidal and midpoint families is shown not to be determinable a priori. Of considerable practical significance, unconditionally stable members of the trapezoidal and midpoint families are identified

A facile one-step fluorescence method for the quantitation of low-content single base deamination impurity in synthetic oligonucleotides.

Science.gov (United States)

Su, Xiaoye; Liang, Ruiting; Stolee, Jessica A

2018-06-05

Oligonucleotides are being researched and developed as potential drug candidates for the treatment of a broad spectrum of diseases. The characterization of antisense oligonucleotide (ASO) impurities caused by base mutations (e.g. deamination) which are closely related to the target ASO is a significant analytical challenge. Herein, we describe a novel one-step method, utilizing a strategy that combines fluorescence-ON detection with competitive hybridization, to achieve single base mutation quantitation in extensively modified synthetic ASOs. Given that this method is highly specific and sensitive (LoQ = 4 nM), we envision that it will find utility for screening other impurities as well as sequencing modified oligonucleotides. Copyright © 2018 Elsevier B.V. All rights reserved.

The research of automatic speed control algorithm based on Green CBTC

Science.gov (United States)

Lin, Ying; Xiong, Hui; Wang, Xiaoliang; Wu, Youyou; Zhang, Chuanqi

2017-06-01

Automatic speed control algorithm is one of the core technologies of train operation control system. It’s a typical multi-objective optimization control algorithm, which achieve the train speed control for timing, comfort, energy-saving and precise parking. At present, the train speed automatic control technology is widely used in metro and inter-city railways. It has been found that the automatic speed control technology can effectively reduce the driver’s intensity, and improve the operation quality. However, the current used algorithm is poor at energy-saving, even not as good as manual driving. In order to solve the problem of energy-saving, this paper proposes an automatic speed control algorithm based on Green CBTC system. Based on the Green CBTC system, the algorithm can adjust the operation status of the train to improve the efficient using rate of regenerative braking feedback energy while ensuring the timing, comfort and precise parking targets. Due to the reason, the energy-using of Green CBTC system is lower than traditional CBTC system. The simulation results show that the algorithm based on Green CBTC system can effectively reduce the energy-using due to the improvement of the using rate of regenerative braking feedback energy.

Comparative studies of upconversion luminescence characteristics and cell bioimaging based on one-step synthesized upconversion nanoparticles capped with different functional groups

Energy Technology Data Exchange (ETDEWEB)

Tsang, Ming-Kiu [Department of Applied Physics, The Hong Kong Polytechnic University, Hong Kong (China); Chan, Chi-Fai; Wong, Ka-Leung [Department of Chemistry, Hong Kong Baptist University (Hong Kong); Hao, Jianhua, E-mail: jh.hao@polyu.edu.hk [Department of Applied Physics, The Hong Kong Polytechnic University, Hong Kong (China)

2015-01-15

Herein, three types of upconverting NaGdF{sub 4}:Yb/Er nanoparticles (UCNPs) have been synthesized via one-step hydrothermal synthesis with polyethylene glycol (PEG), polyethylenimine (PEI) and 6-aminocapronic acid (6AA) functionalization. To evident the presence of these groups, FTIR spectra and ζ-potentials were measured to support the successful capping of PEG, PEI and 6AA on the UCNPs. The regular morphology and cubic phase of these functionalized UCNPs were attributed to the capping effect of the surfactants. Tunable upconversion luminescence (UCL) from red to green were observed under 980 nm laser excitation and the UCL tuning was attributed to the presence of various surface ligands. Moreover, surface group dependent UCL bioimaging was performed in HeLa cells. The enhanced UCL bioimaging demonstrated by PEI functionalized UCNPs evident high cell uptake. The significant cell uptake is explained by the electrostatic attraction between the amino groups (–NH{sub 2}) and the cell membrane. Moreover, the functionalized UCNPs demonstrated low cytotoxicity in MTT assay. Additional, paramagnetic property was presented by these UCNPs under magnetic field. - Highlights: • Tunable upconversion emission by capped functional groups under fixed composition. • Surface dependent upconversion luminescence bioimaging in HeLa cells. • Low cytotoxicity. • Additional paramagnetic property due to Gd{sup 3+} ions.

Challenges of green chemistry in Ukraine

Directory of Open Access Journals (Sweden)

Shevtsova Ganna Ziyvna

2017-06-01

Full Text Available The article deals with study of Ukrainian chemical enterprises’ ecologisation issues and elaboration of the economic problems to realize principles of green chemistry. Theoretical aspects of green chemistry as a modern interdisciplinary conception, which reveals peculiarities to implement sustainable development paradigm in the chemical industry, are studied. Based on the analysis of essence and effectiveness to introduce international initiatives on sustainable development at the chemical industry enterprises, it is concluded that the implemented measures are only first steps on the way to realize key principles of green chemistry.It is proved that in order to promote conceptual ideas of the green chemistry further, it is reasonable to consider economic and marketing aspects of the ecological innovations: to provide economic effectiveness of green chemical products and technologies, to form ecological culture of consumption, to motivate green demand and to prevent market asymmetry of information.

The use of kDNA minicircle subclass relative abundance to differentiate between Leishmania (L.) infantum and Leishmania (L.) amazonensis.

Science.gov (United States)

Ceccarelli, Marcello; Galluzzi, Luca; Diotallevi, Aurora; Andreoni, Francesca; Fowler, Hailie; Petersen, Christine; Vitale, Fabrizio; Magnani, Mauro

2017-05-16

Leishmaniasis is a neglected disease caused by many Leishmania species, belonging to subgenera Leishmania (Leishmania) and Leishmania (Viannia). Several qPCR-based molecular diagnostic approaches have been reported for detection and quantification of Leishmania species. Many of these approaches use the kinetoplast DNA (kDNA) minicircles as the target sequence. These assays had potential cross-species amplification, due to sequence similarity between Leishmania species. Previous works demonstrated discrimination between L. (Leishmania) and L. (Viannia) by SYBR green-based qPCR assays designed on kDNA, followed by melting or high-resolution melt (HRM) analysis. Importantly, these approaches cannot fully distinguish L. (L.) infantum from L. (L.) amazonensis, which can coexist in the same geographical area. DNA from 18 strains/isolates of L. (L.) infantum, L. (L.) amazonensis, L. (V.) braziliensis, L. (V.) panamensis, L. (V.) guyanensis, and 62 clinical samples from L. (L.) infantum-infected dogs were amplified by a previously developed qPCR (qPCR-ML) and subjected to HRM analysis; selected PCR products were sequenced using an ABI PRISM 310 Genetic Analyzer. Based on the obtained sequences, a new SYBR-green qPCR assay (qPCR-ama) intended to amplify a minicircle subclass more abundant in L. (L.) amazonensis was designed. The qPCR-ML followed by HRM analysis did not allow discrimination between L. (L.) amazonensis and L. (L.) infantum in 53.4% of cases. Hence, the novel SYBR green-based qPCR (qPCR-ama) has been tested. This assay achieved a detection limit of 0.1 pg of parasite DNA in samples spiked with host DNA and did not show cross amplification with Trypanosoma cruzi or host DNA. Although the qPCR-ama also amplified L. (L.) infantum strains, the C q values were dramatically increased compared to qPCR-ML. Therefore, the combined analysis of C q values from qPCR-ML and qPCR-ama allowed to distinguish L. (L.) infantum and L. (L.) amazonensis in 100% of tested samples

Detection of mycoplasmas in goat milk by flow cytometry.

Science.gov (United States)

Assunção, Patricia; Davey, Hazel M; Rosales, Ruben S; Antunes, Nuno T; de la Fe, Christian; Ramirez, Ana S; de Galarreta, Carlos M Ruiz; Poveda, Jose B

2007-12-01

The detection of mycoplasma in milk can be performed by either culture techniques or polymerase chain reaction (PCR) based methods. Although PCR can reduce the average diagnostic time to 5 h in comparison with the several days for the isolation of the agent, there is still a need to develop methods, which could give earlier results. For this purpose, we tested the ability of flow cytometry (FC) to detect mycoplasmas in milk samples. Milk samples inoculated with four different mycoplasmas, Mycoplasma agalactiae, Mycoplasma putrefaciens, Mycoplasma capricolum subsp. Capricolum, or Mycoplasma mycoides subsp. mycoides large-colony type, known to cause contagious agalactia in goats, were stained with the DNA stain SYBR Green I and analyzed by FC. Three goat milk samples, from which mycoplasmas have been isolated in broth medium were also analyzed. All mycoplasmas were easily distinguished from debris of milk samples, but it was not possible to distinguish between the different mycoplasma species. In our conditions, the detection limit of the technique was of the order of 10(3)-10(4) cells ml(-1). Furthermore, mycoplasmas were also distinguished from Staphylococcus aureus. FC together with SYBR Green I was able to distinguish between mycoplasma cells and debris present in milk samples and gave results in 20-30 min. This is an important first step in developing a robust, routine flow cytometric method for the detection of mycoplasmas in milk samples. (c) 2007 International Society for Analytical Cytology

Simple One-Step Method to Synthesize Polypyrrole-Indigo Carmine-Silver Nanocomposite

OpenAIRE

Loguercio, Lara Fernandes; Demingos, Pedro; Manica, Luiza de Mattos; Griep, Jordana Borges; Santos, Marcos José Leite; Ferreira, Jacqueline

2016-01-01

A nanocomposite of indigo carmine doped polypyrrole/silver nanoparticles was obtained by a one-step electrochemical process. The nanocomposite was characterized by scanning electron microscopy, infrared spectroscopy, ultraviolet-visible-near infrared spectroscopy, and cyclic voltammetry. The simple one-step process allowed the growth of silver nanoparticles during the polymerization of polypyrrole, resulting in films with electrochromic behavior and improved electroactivity. In addition, poly...

Facile and easily popularized synthesis of L-cysteine-functionalized magnetic nanoparticles based on one-step functionalization for highly efficient enrichment of glycopeptides.

Science.gov (United States)

Feng, Xiaoyan; Deng, Chunhui; Gao, Mingxia; Zhang, Xiangmin

2018-01-01

Protein glycosylation is one of the most important post-translational modifications. Also, efficient enrichment and separation of glycopeptides from complex samples are crucial for the thorough analysis of glycosylation. Developing novel hydrophilic materials with facile and easily popularized synthesis is an urgent need in large-scale glycoproteomics research. Herein, for the first time, a one-step functionalization strategy based on metal-organic coordination was proposed and Fe 3 O 4 nanoparticles were directly functionalized with zwitterionic hydrophilic L-cysteine (L-Cys), greatly simplifying the synthetic procedure. The easily synthesized Fe 3 O 4 /L-Cys possessed excellent hydrophilicity and brief composition, contributing to affinity for glycopeptides and reduction in nonspecific interaction. Thus, Fe 3 O 4 /L-Cys nanoparticles showed outstanding sensitivity (25 amol/μL), high selectivity (mixture of bovine serum albumin and horseradish peroxidase tryptic digests at a mass ratio of 100:1), good reusability (five repeated times), and stability (room temperature storage of 1 month). Encouragingly, in the glycosylation analysis of human serum, a total of 376 glycopeptides with 393 N-glycosylation sites corresponding to 118 glycoproteins were identified after enrichment with Fe 3 O 4 /L-Cys, which was superior to ever reported L-Cys modified magnetic materials. Furthermore, applying the one-step functionalization strategy, cysteamine and glutathione respectively direct-functionalized Fe 3 O 4 nanoparticles were successfully synthesized and also achieved efficient glycopeptide enrichment in human serum. The results indicated that we have presented an efficient and easily popularized strategy in glycoproteomics as well as in the synthesis of novel materials. Graphical abstract Fe 3 O 4 /L-Cys nanoparticles based on one-step functionalization for highly efficient enrichment of glycopeptides.

One-step Ge/Si epitaxial growth.

Science.gov (United States)

Wu, Hung-Chi; Lin, Bi-Hsuan; Chen, Huang-Chin; Chen, Po-Chin; Sheu, Hwo-Shuenn; Lin, I-Nan; Chiu, Hsin-Tien; Lee, Chi-Young

2011-07-01

Fabricating a low-cost virtual germanium (Ge) template by epitaxial growth of Ge films on silicon wafer with a Ge(x)Si(1-x) (0 deposition method in one step by decomposing a hazardousless GeO(2) powder under hydrogen atmosphere without ultra-high vacuum condition and then depositing in a low-temperature region. X-ray diffraction analysis shows that the Ge film with an epitaxial relationship is along the in-plane direction of Si. The successful growth of epitaxial Ge films on Si substrate demonstrates the feasibility of integrating various functional devices on the Ge/Si substrates.

Green roof rainfall-runoff modelling: is the comparison between conceptual and physically based approaches relevant?

Science.gov (United States)

Versini, Pierre-Antoine; Tchiguirinskaia, Ioulia; Schertzer, Daniel

2017-04-01

Green roofs are commonly considered as efficient tools to mitigate urban runoff as they can store precipitation, and consequently provide retention and detention performances. Designed as a compromise between water holding capacity, weight and hydraulic conductivity, their substrate is usually an artificial media differentiating significantly from a traditional soil. In order to assess green roofs hydrological performances, many models have been developed. Classified into two categories (conceptual and physically based), they are usually applied to reproduce the discharge of a particular monitored green roof considered as homogeneous. Although the resulted simulations could be satisfactory, the question of robustness and consistency of the calibrated parameters is often not addressed. Here, a modeling framework has been developed to assess the efficiency and the robustness of both modelling approaches (conceptual and physically based) in reproducing green roof hydrological behaviour. SWMM and VS2DT models have been used for this purpose. This work also benefits from an experimental setup where several green roofs differentiated by their substrate thickness and vegetation cover are monitored. Based on the data collected for several rainfall events, it has been studied how the calibrated parameters are effectively linked to their physical properties and how they can vary from one green roof configuration to another. Although both models reproduce correctly the observed discharges in most of the cases, their calibrated parameters exhibit a high inconsistency. For a same green roof configuration, these parameters can vary significantly from one rainfall event to another, even if they are supposed to be linked to the green roof characteristics (roughness, residual moisture content for instance). They can also be different from one green roof configuration to another although the implemented substrate is the same. Finally, it appears very difficult to find any

Data-Based Predictive Control with Multirate Prediction Step

Science.gov (United States)

Barlow, Jonathan S.

2010-01-01

Data-based predictive control is an emerging control method that stems from Model Predictive Control (MPC). MPC computes current control action based on a prediction of the system output a number of time steps into the future and is generally derived from a known model of the system. Data-based predictive control has the advantage of deriving predictive models and controller gains from input-output data. Thus, a controller can be designed from the outputs of complex simulation code or a physical system where no explicit model exists. If the output data happens to be corrupted by periodic disturbances, the designed controller will also have the built-in ability to reject these disturbances without the need to know them. When data-based predictive control is implemented online, it becomes a version of adaptive control. One challenge of MPC is computational requirements increasing with prediction horizon length. This paper develops a closed-loop dynamic output feedback controller that minimizes a multi-step-ahead receding-horizon cost function with multirate prediction step. One result is a reduced influence of prediction horizon and the number of system outputs on the computational requirements of the controller. Another result is an emphasis on portions of the prediction window that are sampled more frequently. A third result is the ability to include more outputs in the feedback path than in the cost function.

A review of drug delivery systems based on nanotechnology and green chemistry: green nanomedicine.

Science.gov (United States)

Jahangirian, Hossein; Lemraski, Ensieh Ghasemian; Webster, Thomas J; Rafiee-Moghaddam, Roshanak; Abdollahi, Yadollah

2017-01-01

This review discusses the impact of green and environmentally safe chemistry on the field of nanotechnology-driven drug delivery in a new field termed "green nanomedicine". Studies have shown that among many examples of green nanotechnology-driven drug delivery systems, those receiving the greatest amount of attention include nanometal particles, polymers, and biological materials. Furthermore, green nanodrug delivery systems based on environmentally safe chemical reactions or using natural biomaterials (such as plant extracts and microorganisms) are now producing innovative materials revolutionizing the field. In this review, the use of green chemistry design, synthesis, and application principles and eco-friendly synthesis techniques with low side effects are discussed. The review ends with a description of key future efforts that must ensue for this field to continue to grow.

One-step deterministic multipartite entanglement purification with linear optics

Energy Technology Data Exchange (ETDEWEB)

Sheng, Yu-Bo [Department of Physics, Tsinghua University, Beijing 100084 (China); Long, Gui Lu, E-mail: gllong@tsinghua.edu.cn [Department of Physics, Tsinghua University, Beijing 100084 (China); Center for Atomic and Molecular NanoSciences, Tsinghua University, Beijing 100084 (China); Key Laboratory for Quantum Information and Measurements, Beijing 100084 (China); Deng, Fu-Guo [Department of Physics, Applied Optics Beijing Area Major Laboratory, Beijing Normal University, Beijing 100875 (China)

2012-01-09

We present a one-step deterministic multipartite entanglement purification scheme for an N-photon system in a Greenberger–Horne–Zeilinger state with linear optical elements. The parties in quantum communication can in principle obtain a maximally entangled state from each N-photon system with a success probability of 100%. That is, it does not consume the less-entangled photon systems largely, which is far different from other multipartite entanglement purification schemes. This feature maybe make this scheme more feasible in practical applications. -- Highlights: ► We proposed a deterministic entanglement purification scheme for GHZ states. ► The scheme uses only linear optical elements and has a success probability of 100%. ► The scheme gives a purified GHZ state in just one-step.

Design and implementation of the one-step MSD adder of optical computer.

Science.gov (United States)

Song, Kai; Yan, Liping

2012-03-01

On the basis of the symmetric encoding algorithm for the modified signed-digit (MSD), a 7*7 truth table that can be realized with optical methods was developed. And based on the truth table, the optical path structures and circuit implementations of the one-step MSD adder of ternary optical computer (TOC) were designed. Experiments show that the scheme is correct, feasible, and efficient. © 2012 Optical Society of America

Agile project management with GreenHopper 6 blueprints

CERN Document Server

Malik, Jaibeer

2013-01-01

A step-by-step tutorial-based approach.This book is of great help for agile teams who are already using or planning to use the GreenHopper tooling system to execute agile projects. It suits all roles in an agile project including system administrators, stakeholders, product owners, scrum masters, and team members. Fundamental knowledge of JIRA is essential.

Green accounting for greener energy

International Nuclear Information System (INIS)

Stanojevic, M.; Vranes, S.; Goekalp, I.

2010-01-01

The first step towards the widespread use of renewable energy sources and preservation of our environment for the people of the future is to adopt the ''green accounting'' standards that translate socially and environmentally responsible behavior into monetary terms, the only language businesses understand. These standards have the potential of switching on the red light for all pollution-causing power plants, and those depleting the natural capital in any way, be it over-harvesting the forests, or exhausting the underground treasures - coal, oil, natural gas, etc. This paper will show how green accounting can help in changing the focus from the economic welfare to the total societal welfare, acknowledging the fact that human society is an integral part of the natural world. The paper will also briefly present the software developed by the authors that introduce the green accounting principles into the investment appraisal process, aiming at encouraging investments into renewable energy. The tool is also useful as a platform facilitating calibration of economic/financial instruments, like environmental taxes of governmental incentives, that are usually to boost renewable energy sector. The comparative analysis of investment into biofuel-powered combined heat and power production plant using two types of investment valuation standards, one based on conventional cash-flow analysis, the other based on green-accounting standards is detailed in the paper. The analysis is performed as a part of the European Commission Framework Program Project AFTUR, undertaken by the consortium consisting of respectful European Research Establishments in renewable energy area as well as major European biofuel-powered turbine producers. The results show that the wider adoption of green accounting standards would induce the unprecedented growth of the renewable energy sector, because it would make the investment into renewable energy attractive for investors. (author)

Green, one-step and template-free synthesis of silver spongelike networks via a solvothermal method

International Nuclear Information System (INIS)

Yi, Zao; Xu, Xibin; Zhang, Kuibao; Tan, Xiulan; Li, Xibo; Luo, Jiangshan; Ye, Xin; Wu, Weidong; Wu, Jie; Yi, Yougen; Tang, Yongjian

2013-01-01

Silver spongelike networks were synthesized from an alkaline pH solution of silver nitrate and glucose under solvothermal conditions. The products were characterized by X-ray powder diffraction, UV–visible spectroscopy, transmission electron microscopy, scanning electron microscopy and selected area electron diffraction. These Ag nanoparticles (NPs) appear to undergo sequentially linear aggregation and welding initially, and then, they randomly cross link into self-supporting, three-dimensional (3D) networks with time. The carboxylate groups, generated by glucose oxidation, interacted with the Ag nanostructures, resulting in formation of silver spongelike networks having very uniform wire diameters distributions (about 20 nm in diameter). A new plasmon band was observed in the longer-wavelengths region (565–912 nm) of the conventional transverse plasmon resonance band at 430 nm. In principle, this one-step, template-free approach can also be extended to large-scale 3D organizations of other transition/noble metal NPs. - Graphical abstract: Silver spongelike networks were synthesized from an alkaline pH solution of silver nitrate and glucose under solvothermal conditions, with any other reducing or capping agent. These Ag nanoparticles appear to undergo sequentially linear aggregation and welding initially, and then, they randomly cross link into self-supporting, three-dimensional spongelike networks with time. Highlights: ► Silver spongelike networks were synthesized using eco-friendly glucose. ► This synthesis was a seedless process, and did not need any other surfactant or capping agent. ► The process was initial reduction – nucleation – adsorption – growth – branching

Formation of organoclays by a one step synthesis

Science.gov (United States)

Jaber, Maguy; Miéhé-Brendlé, Jocelyne; Delmotte, Luc; Le Dred, Ronan

2005-05-01

Different lamellar hybrid inorganic-organic materials having as inorganic parent 2:1 (T.O.T.) phyllosilicates such as talc, saponite, pyrophyllite, beidellite and montmorillonite were prepared by a one step synthesis. The solids were characterized by X-ray diffraction, solid state 29Si, 27Al, and 19F nuclear magnetic resonance and transmission electron microscopy. XRD patterns show that solids with inorganic parents having octahedral sheet based on aluminium exhibit a lamellar structure similar to MCM-50, whereas those with magnesium have an organophyllosilicate structure. In the first case, the absence of hexacoordinated aluminium was confirmed by 27Al NMR and an ordered stacking of the layers is observed on TEM micrographs. In opposite, a disorder is observed on the TEM images of organophyllosilicates. The formation of the 2:1 structure was found to be controlled mainly by the insertion of silicic species in the interlamellar space of brucite like layers.

One Step Forward and Two Steps Back

DEFF Research Database (Denmark)

Zinner Henriksen, Helle

2018-01-01

A central goal of e-government policy is to increase efficiency in public administration, and one way to increase efficiency is via the increased use of automation and rule-based decision making. The use of data to streamline processes also has a prominent role in public policy today. This chapte...

Genomic prediction for Nordic Red Cattle using one-step and selection index blending

DEFF Research Database (Denmark)

Guosheng, Su; Madsen, Per; Nielsen, Ulrik Sander

2012-01-01

This study investigated the accuracy of direct genomic breeding values (DGV) using a genomic BLUP model, genomic enhanced breeding values (GEBV) using a one-step blending approach, and GEBV using a selection index blending approach for 15 traits of Nordic Red Cattle. The data comprised 6,631 bulls...... genotyped and nongenotyped bulls for one-step blending, and to scale DGV and its expected reliability in the selection index blending. Weighting (scaling) factors had a small influence on reliabilities of GEBV, but a large influence on the variation of GEBV. Based on the validation analyses, averaged over...... the 15 traits, the reliability of DGV for bulls without daughter records was 11.0 percentage points higher than the reliability of conventional pedigree index. Further gain of 0.9 percentage points was achieved by combining information from conventional pedigree index using the selection index blending...

Numerical characterisation of one-step and three-step solar air heating collectors used for cocoa bean solar drying.

Science.gov (United States)

Orbegoso, Elder Mendoza; Saavedra, Rafael; Marcelo, Daniel; La Madrid, Raúl

2017-12-01

In the northern coastal and jungle areas of Peru, cocoa beans are dried using artisan methods, such as direct exposure to sunlight. This traditional process is time intensive, leading to a reduction in productivity and, therefore, delays in delivery times. The present study was intended to numerically characterise the thermal behaviour of three configurations of solar air heating collectors in order to determine which demonstrated the best thermal performance under several controlled operating conditions. For this purpose, a computational fluid dynamics model was developed to describe the simultaneous convective and radiative heat transfer phenomena under several operation conditions. The constructed computational fluid dynamics model was firstly validated through comparison with the data measurements of a one-step solar air heating collector. We then simulated two further three-step solar air heating collectors in order to identify which demonstrated the best thermal performance in terms of outlet air temperature and thermal efficiency. The numerical results show that under the same solar irradiation area of exposition and operating conditions, the three-step solar air heating collector with the collector plate mounted between the second and third channels was 67% more thermally efficient compared to the one-step solar air heating collector. This is because the air exposition with the surface of the collector plate for the three-step solar air heating collector former device was twice than the one-step solar air heating collector. Copyright © 2017 Elsevier Ltd. All rights reserved.

The measurement of unsaturated hydraulic conductivity from one-step outflow method

International Nuclear Information System (INIS)

Lee, S. H.; Hwang, J. H.; Lee, J. M.; Kim, C. R.

2003-01-01

One of the most important parts in constructing radioactive waste repository may be its safety aspect. The fundamental function of the repository is to isolate completely and forever the radioactive wastes disposed of in it. However, since either normally or abnormally nuclides are to be released from the repository with a certain causes. The hydraulic conductivity is related to transportation of nuclide in soil. However, hydraulic characteristics research in unsaturated soil is not enough at present time. A fast and easy procedure for estimating unsaturated flow parameters is presented. The estimation is based on direct measurement of the retention characteristics combined with inverse estimation of the hydraulic conductivity characteristics from one-step outflow experiment

Enhanced planar perovskite solar cell efficiency and stability using a perovskite/PCBM heterojunction formed in one step.

Science.gov (United States)

Zhou, Long; Chang, Jingjing; Liu, Ziye; Sun, Xu; Lin, Zhenhua; Chen, Dazheng; Zhang, Chunfu; Zhang, Jincheng; Hao, Yue

2018-02-08

Perovskite/PCBM heterojunctions are efficient for fabricating perovskite solar cells with high performance and long-term stability. In this study, an efficient perovskite/PCBM heterojunction was formed via conventional sequential deposition and one-step formation processes. Compared with conventional deposition, the one-step process was more facile, and produced a perovskite thin film of substantially improved quality due to fullerene passivation. Moreover, the resulting perovskite/PCBM heterojunction exhibited more efficient carrier transfer and extraction, and reduced carrier recombination. The perovskite solar cell device based on one-step perovskite/PCBM heterojunction formation exhibited a higher maximum PCE of 17.8% compared with that from the conventional method (13.7%). The device also showed exceptional stability, retaining 83% of initial PCE after 60 days of storage under ambient conditions.

Four-year clinical evaluation of Class II nano-hybrid resin composite restorations bonded with a one-step self-etch and a two-step etch-and-rinse adhesive

DEFF Research Database (Denmark)

van Dijken, Jan W V; Pallesen, Ulla

2011-01-01

The objective of this prospective clinical trial was to evaluate the 4-year clinical performance of an ormocer-based nano-hybrid resin composite (Ceram X; Dentsply/DeTrey) in Class II restorations placed with a one-step self-etch (Xeno III; Dentsply/DeTrey) and two-step etch-and-rinse adhesive (I...

Comparison of Gull Feces-specific Assays Targeting the 16S rRNA Gene of Catellicoccus Marimammalium and Streptococcus spp.

Science.gov (United States)

Two novel gull-specific qPCR assays were developed using 16S rRNA gene sequences from gull fecal clone libraries: a SYBR-green-based assay targeting Streptococcus spp. (i.e., gull3) and a TaqMan qPCR assay targeting Catellicoccus marimammalium (i.e., gull4). The main objectives ...

GREEN PACKAGING, GREEN PRODUCT, GREEN ADVERTISING, PERSEPSI, DAN MINAT BELI KONSUMEN

Directory of Open Access Journals (Sweden)

Imam Santoso

2016-12-01

Full Text Available Environmental problems become one of the strategic issues in achieving global competitiveness. One of the issues is products that are made from environmental friendly materials or known as green product. Furthermore, in green products marketing, the company also uses green packaging and green advertising concept. This study aimed to analyze the effect of green packaging, green products, and green advertising on consumer perception and purchasing intention. The study was conducted in Ketawanggede Village, Lowokwaru Sub-district, Malang City. The sampling method used nonprobability accidential sampling techniques. The numbers of respondents were 113 consumers in study site. Data were collected by interview using questionnaires. The method of analysis used Generalized Structured Component Analysis (GSCA. The analysis showed that the green packaging, green products, and green advertising had positive significant influence on consumer perceptions. Meanwhile, green product and consumer perception had positive significant influence on purchasing interest, but the green packaging and green advertising has not found sufficient evidence in influencing purchasing intention.

Scattering theory for one-dimensional step potentials

International Nuclear Information System (INIS)

Ruijsenaars, S.N.M.; Bongaarts, P.J.M.

1977-01-01

The scattering theory is treated for the one-dimensional Dirac equation with potentials that are bounded, measurable, real-valued functions on the real line, having constant values, not necessarily the same, on the left and on the right side of a compact interval. Such potentials appear in the Klein paradox. It is shown that appropriately modified wave operators exist and that the corresponding S-operator is unitary. The connection between time-dependent scattering theory and time-independent scattering theory in terms of incoming and outgoing plane wave solutions is established and some further properties are proved. All results and their proofs have a straightforward translation to the one-dimensional Schroedinger equation with the same class of step potentials

One-step synthesis of solid state luminescent carbon-based silica nanohybrids for imaging of latent fingerprints

Science.gov (United States)

Li, Feng; Li, Hongren; Cui, Tianfang

2017-11-01

Fluorescent carbon-based nanomaterials(CNs) with tunable visible emission are biocompatible, environment friendly and most suitable for various biomedical applications. Despite the successes in preparing strongly fluorescent CNs, preserving the luminescence in solid materials is still challenging because of the serious emission quenching of CNs in solid state materials. In this work, fluorescent carbon and silica nanohybrids (SiCNHs) were synthesized via a simple one-step hydrothermal approach by carbonizing sodium citrate and (3-aminopropyl)triethoxysilane(APTES), and hydrolysis of tetraethyl orthosilicate(TEOS). The resultant SiCNs were characterized through X-ray diffraction (XRD), transmission electron microscopy (TEM), FT-IR, X-ray photoelectron spectroscopy (XPS), and photoluminescence (PL) spectroscopy. The SiCNs exhibited strong fluorescence in both aqueous and solid states. The luminescent solid state SiCNs power were successfully used as a fluorescent labeling material for enhanced imaging of latent fingerprints(LFPs) on single background colour and multi-coloured surfaces substrates in forensic science for individual identification.

Structural Studies of Silver Nanoparticles Obtained Through Single-Step Green Synthesis

Science.gov (United States)

Prasad Peddi, Siva; Abdallah Sadeh, Bilal

2015-10-01

Green synthesis of silver Nanoparticles (AGNP's) has been the most prominent among the metallic nanoparticles for research for over a decade and half now due to both the simplicity of preparation and the applicability of biological species with extensive applications in medicine and biotechnology to reduce and trap the particles. The current article uses Eclipta Prostrata leaf extract as the biological species to cap the AGNP's through a single step process. The characterization data obtained was used for the analysis of the sample structure. The article emphasizes the disquisition of their shape and size of the lattice parameters and proposes a general scheme and a mathematical model for the analysis of their dependence. The data of the synthesized AGNP's has been used to advantage through the introduction of a structural shape factor for the crystalline nanoparticles. The properties of the structure of the AGNP's proposed and evaluated through a theoretical model was undeviating with the experimental consequences. This modus operandi gives scope for the structural studies of ultrafine particles prepared using biological methods.

Effect of evaporation of solvents from one-step, self-etching adhesives

DEFF Research Database (Denmark)

Furuse, Adilson Yoshio; Peutzfeldt, Anne; Asmussen, Erik

2008-01-01

PURPOSE: To investigate whether and to what extent the bonding capacity of one-step, self-etching adhesives is influenced by the degree to which solvent is evaporated. MATERIALS AND METHODS: Seven one-step, self-etching adhesives were tested (Adper Prompt L-Pop, Clearfil S3 Bond, Futurabond NR, G....... After being stored in water at 37 degrees C for 1 week, the bonded specimens were broken in shear. Failure modes were evaluated under stereomicroscope. RESULTS: Air-blowing duration and brand of adhesive both had an effect on shear bond strength. An interaction was found between adhesive and air...... failures were observed with shorter air-blowing durations. A significant negative correlation between number of adhesive failures and bond strength was found. CONCLUSION: On the basis of this in vitro study, it may be concluded that the one-step, self-etching adhesives evaluated were sensitive to degree...

Off-Shell Green Functions: One-Loop with Growing Legs

International Nuclear Information System (INIS)

Bashir, A.; Concha-Sanchez, Y.; Delbourgo, R.; Tejeda-Yeomans, M. E.

2008-01-01

One loop calculations in gauge theories in arbitrary gauge and dimensions become exceedingly hard with growing number of external off-shell legs. Let alone higher point functions, such a calculation for even the three point one-loop vertices for quantum electrodynamics (QED) and quantum chromodynamics (QCD) has been made available only recently. In this article, we discuss how Ward-Fradkin-Green-Takahashi identities (WFGTI) may provide a helpful tool in these computations. After providing a glimpse of our suggestion for the case of the 3-point vertex, we present our preliminary findings towards our similar efforts for the 4-point function. We restrict ourselves to the example of scalar quantum electrodynamics (SQED)

Simple One-Step Method to Synthesize Polypyrrole-Indigo Carmine-Silver Nanocomposite

Directory of Open Access Journals (Sweden)

Lara Fernandes Loguercio

2016-01-01

Full Text Available A nanocomposite of indigo carmine doped polypyrrole/silver nanoparticles was obtained by a one-step electrochemical process. The nanocomposite was characterized by scanning electron microscopy, infrared spectroscopy, ultraviolet-visible-near infrared spectroscopy, and cyclic voltammetry. The simple one-step process allowed the growth of silver nanoparticles during the polymerization of polypyrrole, resulting in films with electrochromic behavior and improved electroactivity. In addition, polypyrrole chains in the nanocomposite were found to present longer conjugation length than pristine polypyrrole films.

Stability of one-step methods in transient nonlinear heat conduction

International Nuclear Information System (INIS)

Hughes, J.R.

1977-01-01

The purpose of the present work is to ascertain practical stability conditions for one-step methods commonly used in transient nonlinear heat conduction analyses. In this paper the concepts of stability, appropriate to the nonlinear problem, are thoroughly discussed. They of course reduce to the usual stability critierion for the linear, constant coefficient case. However, for nonlinear problems there are differences and theses ideas are of key importance in obtaining practical stability conditions. Of particular importance is a recent result which indicates that, in a sense, the trapezoidal and midpoint families are equivalent. Thus, stability results for one family may be translated into a result for the other. The main results obtained are: The stability behaviour of the explicit Euler method in the nonlinear regime is analogous to that for linear problems. In particular, an a priori step size restriction may be determined for each time step. The precise time step restriction on implicit conditionally stable members of the trapezoidal and midpoint families is shown not to be determinable a priori. Of considerable practical significance, unconditionally stable members of the trapezoidal and midpoint families are identified. All notions of stability employed are motivated and defined, and their interpretations in practical computing are indicated. (Auth.)

Doubled Shapiro steps in a topological Josephson junction

Science.gov (United States)

Li, Yu-Hang; Song, Juntao; Liu, Jie; Jiang, Hua; Sun, Qing-Feng; Xie, X. C.

2018-01-01

We study the transport properties of a superconductor-quantum spin Hall insulator-superconductor hybrid system in the presence of microwave radiation. Instead of adiabatic analysis or use of the resistively shunted junction model, we start from the microscopic Hamiltonian and calculate the d.c. current directly with the help of the nonequilibrium Green's function method. The numerical results show that (i) the I-V curves of background current due to multiple Andreev reflections exhibit a different structure from those in the conventional junctions, and (ii) all Shapiro steps are visible and appear one by one at high frequencies, while at low frequencies, the steps evolve exactly as the Bessel functions and the odd steps are completely suppressed, implying a fractional Josephson effect.

Step-by-Step Simulation of Radiation of Radiation Chemistry Using Green Functions for Diffusion-Influenced Reactions

Science.gov (United States)

Plante, Ianik; Cucinotta, Francis A.

2011-01-01

The irradiation of biological systems leads to the formation of radiolytic species such as H(raised dot), (raised dot)OH, H2, H2O2, e(sup -)(sub aq), etc.[1]. These species react with neighboring molecules, which result in damage in biological molecules such as DNA. Radiation chemistry is there for every important to understand the radiobiological consequences of radiation[2]. In this work, we discuss an approach based on the exact Green Functions for diffusion-influenced reactions which may be used to simulate radiation chemistry and eventually extended to study more complex systems, including DNA.

Rapid detection of Enterovirus and Coxsackievirus A10 by a TaqMan based duplex one-step real time RT-PCR assay.

Science.gov (United States)

Chen, Jingfang; Zhang, Rusheng; Ou, Xinhua; Yao, Dong; Huang, Zheng; Li, Linzhi; Sun, Biancheng

2017-06-01

A TaqMan based duplex one-step real time RT-PCR (rRT-PCR) assay was developed for the rapid detection of Coxsackievirus A10 (CV-A10) and other enterovirus (EVs) in clinical samples. The assay was fully evaluated and found to be specific and sensitive. When applied in 115 clinical samples, a 100% diagnostic sensitivity in CV-A10 detection and 97.4% diagnostic sensitivity in other EVs were found. Copyright © 2017 Elsevier Ltd. All rights reserved.

Rapid one-step selection method for generating nucleic acid aptamers: development of a DNA aptamer against α-bungarotoxin.

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Lasse H Lauridsen

Full Text Available BACKGROUND: Nucleic acids based therapeutic approaches have gained significant interest in recent years towards the development of therapeutics against many diseases. Recently, research on aptamers led to the marketing of Macugen®, an inhibitor of vascular endothelial growth factor (VEGF for the treatment of age related macular degeneration (AMD. Aptamer technology may prove useful as a therapeutic alternative against an array of human maladies. Considering the increased interest in aptamer technology globally that rival antibody mediated therapeutic approaches, a simplified selection, possibly in one-step, technique is required for developing aptamers in limited time period. PRINCIPAL FINDINGS: Herein, we present a simple one-step selection of DNA aptamers against α-bungarotoxin. A toxin immobilized glass coverslip was subjected to nucleic acid pool binding and extensive washing followed by PCR enrichment of the selected aptamers. One round of selection successfully identified a DNA aptamer sequence with a binding affinity of 7.58 µM. CONCLUSION: We have demonstrated a one-step method for rapid production of nucleic acid aptamers. Although the reported binding affinity is in the low micromolar range, we believe that this could be further improved by using larger targets, increasing the stringency of selection and also by combining a capillary electrophoresis separation prior to the one-step selection. Furthermore, the method presented here is a user-friendly, cheap and an easy way of deriving an aptamer unlike the time consuming conventional SELEX-based approach. The most important application of this method is that chemically-modified nucleic acid libraries can also be used for aptamer selection as it requires only one enzymatic step. This method could equally be suitable for developing RNA aptamers.

One size fits all electronics for insole-based activity monitoring.

Science.gov (United States)

Hegde, Nagaraj; Bries, Matthew; Melanson, Edward; Sazonov, Edward

2017-07-01

Footwear based wearable sensors are becoming prominent in many areas of monitoring health and wellness, such as gait and activity monitoring. In our previous research we introduced an insole based wearable system SmartStep, which is completely integrated in a socially acceptable package. From a manufacturing perspective, SmartStep's electronics had to be custom made for each shoe size, greatly complicating the manufacturing process. In this work we explore the possibility of making a universal electronics platform for SmartStep - SmartStep 3.0, which can be used in the most common insole sizes without modifications. A pilot human subject experiments were run to compare the accuracy between the one-size fits all (SmartStep 3.0) and custom size SmartStep 2.0. A total of ~10 hours of data was collected in the pilot study involving three participants performing different activities of daily living while wearing SmartStep 2.0 and SmartStep 3.0. Leave one out cross validation resulted in a 98.5% average accuracy from SmartStep 2.0, while SmartStep 3.0 resulted in 98.3% accuracy, suggesting that the SmartStep 3.0 can be as accurate as SmartStep 2.0, while fitting most common shoe sizes.

Investigation of hemorrhagic fever viruses inside wild populations of ticks: One of the pioneer studies in Saudi Arabia

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Rania Ali El Hadi Mohamed

2017-05-01

Full Text Available Objective: To screen hemorrhagic fever viruses inside wild populations of ticks collected from Riyadh, Saudi Arabia between January and March 2016. Methods: Ticks were identified depending on their morphological features using classical keys then grouped into pools. Ticks in each pool were processed separately using the sterile pestles and mortars. Viral RNA was extracted using Qiagen RNeasy Mini Kit and Qiagen RNAeasy Columns (Qiagen, Hilden, Germany according to the instructions of manufacturers. A total number of 1 282 hard ticks were collected, and 582 of them were precisely identified then screened for the presence of arboviruses using quantitative real-time PCR. The four species were screened for six viruses: Rift Valley fever virus (RVFV, Chikungunya virus (CHIKV, Crimean-Congo hemorrhagic fever virus (CCHFV, Alkhurma virus (INKV, Sindbis virus (SINV, and Pan Hanta virus (HANTA. CT value for the negative control (RNA free water was zero. Negative and positive controls were tested for each test to confirm the specificity of the selected primer pairs. SYBR Green One step RT-PCR Master Mix (KAPA Biosystems, Boston, MA was tested along with primers. Results: Ticks identification resulted into four species: Hyalomma schulzei, Hyalomma onatoli, Boophilus kdhlsi, and Hyalomm dromedarii. All the ticks’ species (except Boophilus kdhlsi were positive for the following viruses: SINV, RVFV, CHIKV, and CCHFV. While HANTA viruses have been detected in a single species (Hyalomm dromedarii. Conclusions: According to our knowledge this research may be one of the pioneer studies in Kingdom of Saudi Arabia. Incrimination of the above mentioned ticks species as well as their vectorial capacity are highly recommended for investigation in the upcoming researches.

Helicase and Polymerase Move Together Close to the Fork Junction and Copy DNA in One-Nucleotide Steps

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Manjula Pandey

2014-03-01

Full Text Available By simultaneously measuring DNA synthesis and dNTP hydrolysis, we show that T7 DNA polymerase and T7 gp4 helicase move in sync during leading-strand synthesis, taking one-nucleotide steps and hydrolyzing one dNTP per base-pair unwound/copied. The cooperative catalysis enables the helicase and polymerase to move at a uniformly fast rate without guanine:cytosine (GC dependency or idling with futile NTP hydrolysis. We show that the helicase and polymerase are located close to the replication fork junction. This architecture enables the polymerase to use its strand-displacement synthesis to increase the unwinding rate, whereas the helicase aids this process by translocating along single-stranded DNA and trapping the unwound bases. Thus, in contrast to the helicase-only unwinding model, our results suggest a model in which the helicase and polymerase are moving in one-nucleotide steps, DNA synthesis drives fork unwinding, and a role of the helicase is to trap the unwound bases and prevent DNA reannealing.

Lyondell develops one step isobutylene process

International Nuclear Information System (INIS)

Anon.

1992-01-01

This paper reports that Lyondell Petrochemical Co., Houston, has developed a one step process to convert normal butylenes to isobutylene, a key component of methyl tertiary butyl ether (MTBE). MTBE is expected to become the additive of choice among U.S. refiners to blend oxygenated gasolines required by 1990 amendments to the Clean Air Act. Lyondell Pres. and Chief Executive Officer Bob Gower the the new process could help assure adequate supplies of MTBE to meet U.S. demand for cleaner burning fuels. Lyondell estimates the capital cost of building a grassroots plant to produce isobutylene with the new process would be less than half the cost of a grassroot plant to produce isobutylene with existing technology starting with normal butane

Research on the influence of institutional pressures on green innovation strategy

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Zewen, Chen; xin, Li; Hongjun, Cao

2017-11-01

Based on the new Institutional theory and the sample of 116 enterprises, this paper explores the influencing factors of green innovation strategy from the perspective of forced pressure, normative pressure and imitation pressure. The results show that the mandatory regulation, the incentive regulation, the supply chain pressure, and the competitive pressure all have a significant and positive impact on the green innovation strategy. Therefore, the government should take steps to stimulate enterprises to choose the green innovation strategy.

Implementation Opportunities of Green Accounting for Activity-Based Costing (ABC in Romania

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Sorinel Capusneanu

2008-01-01

Full Text Available This article refers to implementation opportunities of green accounting for the Activity- Based Costing method. It shows why we choose Activity-Based Costing method and what must be done in this way. Green accounting observes the specific principles of the Activity-Based Costing method. It also represents the advantages and disadvantages of the green accounting into an enterprise in case of Activity-Based Costing implementation. The paper describes the stages we must follow in case of implementation of green accounting alongside Activity-Based Costing method into an enterprise.

Green's function method and its application to verification of diffusion models of GASFLOW code

International Nuclear Information System (INIS)

Xu, Z.; Travis, J.R.; Breitung, W.

2007-07-01

To validate the diffusion model and the aerosol particle model of the GASFLOW computer code, theoretical solutions of advection diffusion problems are developed by using the Green's function method. The work consists of a theory part and an application part. In the first part, the Green's functions of one-dimensional advection diffusion problems are solved in infinite, semi-infinite and finite domains with the Dirichlet, the Neumann and/or the Robin boundary conditions. Novel and effective image systems especially for the advection diffusion problems are made to find the Green's functions in a semi-infinite domain. Eigenfunction method is utilized to find the Green's functions in a bounded domain. In the case, key steps of a coordinate transform based on a concept of reversed time scale, a Laplace transform and an exponential transform are proposed to solve the Green's functions. Then the product rule of the multi-dimensional Green's functions is discussed in a Cartesian coordinate system. Based on the building blocks of one-dimensional Green's functions, the multi-dimensional Green's function solution can be constructed by applying the product rule. Green's function tables are summarized to facilitate the application of the Green's function. In the second part, the obtained Green's function solutions benchmark a series of validations to the diffusion model of gas species in continuous phase and the diffusion model of discrete aerosol particles in the GASFLOW code. Perfect agreements are obtained between the GASFLOW simulations and the Green's function solutions in case of the gas diffusion. Very good consistencies are found between the theoretical solutions of the advection diffusion equations and the numerical particle distributions in advective flows, when the drag force between the micron-sized particles and the conveying gas flow meets the Stokes' law about resistance. This situation is corresponding to a very small Reynolds number based on the particle

Facile fabrication of three-dimensional mesoporous Si/SiC composites via one-step magnesiothermic reduction at relative low temperature

Energy Technology Data Exchange (ETDEWEB)

Jiang, Zhihang; Ma, Yongjun [State Key Laboratory Cultivation Base for Nonmetal Composites and Functional Materials, Southwest University of Science and Technology, Mianyang 621010 (China); Zhou, Yong [Eco-materials and Renewable Energy Research Center (ERERC), School of Physics, National Lab of Solid State Microstructure, ERERC, Nanjing University, Nanjing 210093 (China); Hu, Shanglian [School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010 (China); Han, Chaojiang [State Key Laboratory Cultivation Base for Nonmetal Composites and Functional Materials, Southwest University of Science and Technology, Mianyang 621010 (China); Pei, Chonghua, E-mail: peichonghua@swust.edu.cn [State Key Laboratory Cultivation Base for Nonmetal Composites and Functional Materials, Southwest University of Science and Technology, Mianyang 621010 (China)

2013-10-15

Graphical abstract: - Highlights: • The Si/SiC composites were synthesized by one-step magnesiothermic reduction. • The mesoporous composites have a high specific surface area (655.7 m{sup 2} g{sup −1}). • The composites exhibited a strong photoluminescence and better biocompatibility. • The mechanisms of formation and photoluminescence of sample were discussed. - Abstract: By converting modified silica aerogels to the corresponding silicon/silicon carbide (Si/SiC) without losing its nanostructure, three-dimensional mesoporous (3DM) Si/SiC composites are successfully synthesized via one-step magnesothermic reduction at relative low temperature (650 °C). The phase composition and microstructure of the resulting samples are measured by X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDX), Raman spectra, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). N{sub 2}-sorption isotherms results show that the products have high Brunauer–Emmett–Teller (BET) specific surface areas (up to 656 m{sup 2} g{sup −1}) and narrow pore-size distributions (1.5–30 nm). The composites exhibit a strong photoluminescence (PL) in blue-green light region (peak centered at 533 nm). We have set out work on the biocompatibility and enhancing PL of samples. As a result of excellent performances of the composites, it can be expected to have significant application in optoelectronics, biosensors, biological tracer and so on.

Hole transport in c-plane InGaN-based green laser diodes

Energy Technology Data Exchange (ETDEWEB)

Cheng, Yang; Liu, Jianping, E-mail: jpliu2010@sinano.ac.cn; Tian, Aiqin; Zhang, Feng; Feng, Meixin; Hu, Weiwei; Zhang, Shuming; Ikeda, Masao; Li, Deyao; Zhang, Liqun; Yang, Hui [Key Lab of Nanodevices and Applications, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences (CAS), Suzhou 215123 (China); School of Nano Technology and Nano Bionics, University of Science and Technology of China, Suzhou 215123 (China)

2016-08-29

Hole transport in c-plane InGaN-based green laser diodes (LDs) has been investigated by both simulations and experiments. It is found that holes can overflow from the green double quantum wells (DQWs) at high current density, which reduces carrier injection efficiency of c-plane InGaN-based green LDs. A heavily silicon-doped layer right below the green DQWs can effectively suppress hole overflow from the green DQWs.

The political economy of international green certificate markets

International Nuclear Information System (INIS)

Soederholm, Patrik

2008-01-01

This paper analyzes the political economy of establishing bilateral trade in green certificate markets as one step towards harmonization of European green electricity support systems. We outline some of the economic principles of an integrated bilateral green certificates market, and then discuss a number of issues that are deemed to be critical for the effectiveness, stability and legitimacy of such a market. By drawing on some of the lessons of the fairly recent intentions to integrate a future green certificate market in Norway with the existing Swedish one, we highlight, exemplify and discuss some critical policy implementation and design issues. These include, for instance, the system's connection to climate policy targets, the role of other support schemes and the definition of what green electricity technologies should be included. Furthermore, the establishment of an international market presumes that the benefits of renewable power (e.g., its impacts on the environment, diversification of the power mix, self-sufficiency, etc.) are approached and valued from an international perspective rather than from a national one, thus implying lesser emphasis on, for instance, employment and regional development impacts. A bilateral green certificate system thus faces a number of important policy challenges, but at the same time it could provide important institutional learning effects that can be useful for future attempts aiming at achieving greater policy integration in the European renewable energy sector

Green telecom technology (GCT): think green - a step to achieve improved techno-environmental and socio-economic effects in Pakistan

International Nuclear Information System (INIS)

Anwar, C.M.

2010-01-01

The purpose of this research is development of know-how about the worldwide techno- environmental effects of telecom/lCT industry and proposal of a strategy to cope with this hazardous issue. Our main focus is to develop a strategy to minimize the pollution-level (Energy-consumption, Waste, Green-House Gases (GHG) emission, including CO/sub 2/) in the environment produced by telecom/lCT industry of Pakistan. We want to save our environment by introducing not only environment-friendly Green Telecom Technology (GTT), but our main theme is to convert the thinking of our carriers and public from black to green technologies. We shall analyze the socio-economic effects of going green. This research also suggests the concept of green-tax to government, which could be helpful to increase government equity. (author)

One Step Closer to the Marketplace for State-of-the-Art Wind Turbine

Science.gov (United States)

Drivetrain | News | NREL One Step Closer to the Marketplace for State-of-the-Art Wind Turbine Drivetrain One Step Closer to the Marketplace for State-of-the-Art Wind Turbine Drivetrain April 1, 2016 modeling, and testing in state-of-the-art facilities designed to put the drivetrain through its paces

Variable Step Size Maximum Correntropy Criteria Based Adaptive Filtering Algorithm

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S. Radhika

2016-04-01

Full Text Available Maximum correntropy criterion (MCC based adaptive filters are found to be robust against impulsive interference. This paper proposes a novel MCC based adaptive filter with variable step size in order to obtain improved performance in terms of both convergence rate and steady state error with robustness against impulsive interference. The optimal variable step size is obtained by minimizing the Mean Square Deviation (MSD error from one iteration to the other. Simulation results in the context of a highly impulsive system identification scenario show that the proposed algorithm has faster convergence and lesser steady state error than the conventional MCC based adaptive filters.

Genus one super-Green function revisited and superstring amplitudes with non-maximal supersymmetry

International Nuclear Information System (INIS)

Itoyama, H.; Yano, Kohei

2016-01-01

We reexamine genus one super-Green functions with general boundary conditions twisted by (α,β) for (σ,τ) directions in the eigenmode expansion and derive expressions as infinite series of hypergeometric functions. Using these, we compute one-loop superstring amplitudes with non-maximal supersymmetry, taking the example of massless vector emissions of open string type I Z 2 orbifold

One Giant Leap for Categorizers: One Small Step for Categorization Theory

Science.gov (United States)

Smith, J. David; Ell, Shawn W.

2015-01-01

We explore humans’ rule-based category learning using analytic approaches that highlight their psychological transitions during learning. These approaches confirm that humans show qualitatively sudden psychological transitions during rule learning. These transitions contribute to the theoretical literature contrasting single vs. multiple category-learning systems, because they seem to reveal a distinctive learning process of explicit rule discovery. A complete psychology of categorization must describe this learning process, too. Yet extensive formal-modeling analyses confirm that a wide range of current (gradient-descent) models cannot reproduce these transitions, including influential rule-based models (e.g., COVIS) and exemplar models (e.g., ALCOVE). It is an important theoretical conclusion that existing models cannot explain humans’ rule-based category learning. The problem these models have is the incremental algorithm by which learning is simulated. Humans descend no gradient in rule-based tasks. Very different formal-modeling systems will be required to explain humans’ psychology in these tasks. An important next step will be to build a new generation of models that can do so. PMID:26332587

Properties of the reverse transcription reaction in mRNA quantification

DEFF Research Database (Denmark)

Ståhlberg, Anders; Håkansson, Joakim; Xian, Xiaojie

2004-01-01

BACKGROUND: In most measurements of gene expression, mRNA is first reverse-transcribed into cDNA. We studied the reverse transcription reaction and its consequences for quantitative measurements of gene expression. METHODS: We used SYBR green I-based quantitative real-time PCR (QPCR) to measure...... the properties of reverse transcription reaction for the beta-tubulin, glyceraldehyde-3-phosphate dehydrogenase, Glut2, CaV1D, and insulin II genes, using random hexamers, oligo(dT), and gene-specific reverse transcription primers. RESULTS: Experimental variation in reverse transcription-QPCR (RT......-QPCR) was mainly attributable to the reverse transcription step. Reverse transcription efficiency depended on priming strategy, and the dependence was different for the five genes studied. Reverse transcription yields also depended on total RNA concentration. CONCLUSIONS: RT-QPCR gene expression measurements...

A review of drug delivery systems based on nanotechnology and green chemistry: green nanomedicine

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Jahangirian H

2017-04-01

Full Text Available Hossein Jahangirian,1 Ensieh Ghasemian Lemraski,2 Thomas J Webster,1 Roshanak Rafiee-Moghaddam,3 Yadollah Abdollahi4 1Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 2Department of Chemistry, Faculty of Science, Ilam University, Ilam, Iran; 3School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor, 4Department of Electrical Engineering, Faculty of Engineering, University of Malaysia, Kuala Lumpur, Malaysia Abstract: This review discusses the impact of green and environmentally safe chemistry on the field of nanotechnology-driven drug delivery in a new field termed “green nanomedicine”. Studies have shown that among many examples of green nanotechnology-driven drug delivery systems, those receiving the greatest amount of attention include nanometal particles, polymers, and biological materials. Furthermore, green nanodrug delivery systems based on environmentally safe chemical reactions or using natural biomaterials (such as plant extracts and microorganisms are now producing innovative materials revolutionizing the field. In this review, the use of green chemistry design, synthesis, and application principles and eco-friendly synthesis techniques with low side effects are discussed. The review ends with a description of key future efforts that must ensue for this field to continue to grow. Keywords: green chemistry, cancer, drug delivery, nanoparticle

Novel one-pot one-step synthesis of 2'-[(18)F]fluoroflumazenil (FFMZ) for benzodiazepine receptor imaging.

Science.gov (United States)

Yoon, Young Hyun; Jeong, Jae Min; Kim, Hyung Woo; Hong, Sung Hyun; Lee, Yun-Sang; Kil, Hee Sup; Chi, Dae Yoon; Lee, Dong Soo; Chung, June-Key; Lee, Myung Chul

2003-07-01

We describe the synthesis of 2'-[(18)F]fluoroflumazenil (FFMZ), which differs from the typically used [(18)F]fluoroethylflumazenil (FEFMZ) for benzodiazepine receptor imaging. For one-pot one-step labeling, the precursors, 2'-tosyloxyflumazenil (TFMZ) and 2'-mesyloxyflumazenil (MFMZ), were synthesized in three steps. The precursors were successfully labeled with no-carrier-added (18)F-fluoride which was activated by repeated azeotropic distillation with Kryptofix 2.2.2./potassium carbonate in MeCN. An automated system for labeling and purification of [(18)F]FFMZ was developed. Labeling efficiency and radiochemical purity of [(18)F]FFMZ after synthesis by the automated system were 68% and 98%, respectively. Specific binding of [(18)F]FFMZ to central benzodiazepine receptor of rats was demonstrated by phosphoimaging.

Novel one-pot one-step synthesis of 2'-[18F]fluoroflumazenil (FFMZ) for benzodiazepine receptor imaging

International Nuclear Information System (INIS)

Young, Hyun Yoon; Jae, Min Jeong; Hyung, Woo Kim; Sung, Hyun Hong; Lee, Yun-Sang; Hee, Sup Kil; Dae, Yoon Chi; Dong, Soo Lee; Chung, June-Key; Myung, Chul Lee

2003-01-01

We describe the synthesis of 2'-[ 18 F]fluoroflumazenil (FFMZ), which differs from the typically used [ 18 F]fluoroethylflumazenil (FEFMZ) for benzodiazepine receptor imaging. For one-pot one-step labeling, the precursors, 2'-tosyloxyflumazenil (TFMZ) and 2'-mesyloxyflumazenil (MFMZ), were synthesized in three steps. The precursors were successfully labeled with no-carrier-added 18 F-fluoride which was activated by repeated azeotropic distillation with Kryptofix 2.2.2./potassium carbonate in MeCN. An automated system for labeling and purification of [ 18 F]FFMZ was developed. Labeling efficiency and radiochemical purity of [ 18 F]FFMZ after synthesis by the automated system were 68% and 98%, respectively. Specific binding of [ 18 F]FFMZ to central benzodiazepine receptor of rats was demonstrated by phosphoimaging

Using Green Star Metrics to Optimize the Greenness of Literature Protocols for Syntheses

Science.gov (United States)

Duarte, Rita C. C.; Ribeiro, M. Gabriela T. C.; Machado, Adélio A. S. C.

2015-01-01

A procedure to improve the greenness of a synthesis, without performing laboratory work, using alternative protocols available in the literature is presented. The greenness evaluation involves the separate assessment of the different steps described in the available protocols--reaction, isolation, and purification--as well as the global process,…

Quantification of massively parallel sequencing libraries - a comparative study of eight methods

DEFF Research Database (Denmark)

Hussing, Christian; Kampmann, Marie-Louise; Mogensen, Helle Smidt

2018-01-01

Quantification of massively parallel sequencing libraries is important for acquisition of monoclonal beads or clusters prior to clonal amplification and to avoid large variations in library coverage when multiple samples are included in one sequencing analysis. No gold standard for quantification...... estimates followed by Qubit and electrophoresis-based instruments (Bioanalyzer, TapeStation, GX Touch, and Fragment Analyzer), while SYBR Green and TaqMan based qPCR assays gave the lowest estimates. qPCR gave more accurate predictions of sequencing coverage than Qubit and TapeStation did. Costs, time......-consumption, workflow simplicity, and ability to quantify multiple samples are discussed. Technical specifications, advantages, and disadvantages of the various methods are pointed out....

Canine distemper virus detection by different methods of One-Step RT-qPCR

Directory of Open Access Journals (Sweden)

Claudia de Camargo Tozato

2016-01-01

Full Text Available ABSTRACT: Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Canine Distemper Virus. Using the kit that showed better performance, two systems of Real-time RT-PCR (RT-qPCR assays were tested and compared for analytical sensitivity to Canine Distemper Virus RNA detection: a One-Step RT-qPCR (system A and a One-Step RT-qPCR combined with NESTED-qPCR (system B. Limits of detection for both systems were determined using a serial dilution of Canine Distemper Virus synthetic RNA or a positive urine sample. In addition, the same urine sample was tested using samples with prior centrifugation or ultracentrifugation. Commercial kits of One-Step RT-qPCR assays detected canine distemper virus RNA in 10 (100% urine samples from symptomatic animals tested. The One-Step RT-qPCR kit that showed better results was used to evaluate the analytical sensitivity of the A and B systems. Limit of detection using synthetic RNA for the system A was 11 RNA copies µL-1 and 110 RNA copies µl-1 for first round System B. The second round of the NESTED-qPCR for System B had a limit of detection of 11 copies µl-1. Relationship between Ct values and RNA concentration was linear. The RNA extracted from the urine dilutions was detected in dilutions of 10-3 and10-2 by System A and B respectively. Urine centrifugation increased the analytical sensitivity of the test and proved to be useful for routine diagnostics. The One-Step RT-qPCR is a fast, sensitive and specific method for canine distemper routine diagnosis and research projects that require sensitive and quantitative methodology.

One-step sol-gel imprint lithography for guided-mode resonance structures.

Science.gov (United States)

Huang, Yin; Liu, Longju; Johnson, Michael; C Hillier, Andrew; Lu, Meng

2016-03-04

Guided-mode resonance (GMR) structures consisting of sub-wavelength periodic gratings are capable of producing narrow-linewidth optical resonances. This paper describes a sol-gel-based imprint lithography method for the fabrication of submicron 1D and 2D GMR structures. This method utilizes a patterned polydimethylsiloxane (PDMS) mold to fabricate the grating coupler and waveguide for a GMR device using a sol-gel thin film in a single step. An organic-inorganic hybrid sol-gel film was selected as the imprint material because of its relatively high refractive index. The optical responses of several sol-gel GMR devices were characterized, and the experimental results were in good agreement with the results of electromagnetic simulations. The influence of processing parameters was investigated in order to determine how finely the spectral response and resonant wavelength of the GMR devices could be tuned. As an example potential application, refractometric sensing experiments were performed using a 1D sol-gel device. The results demonstrated a refractive index sensitivity of 50 nm/refractive index unit. This one-step fabrication process offers a simple, rapid, and low-cost means of fabricating GMR structures. We anticipate that this method can be valuable in the development of various GMR-based devices as it can readily enable the fabrication of complex shapes and allow the doping of optically active materials into sol-gel thin film.

One-step sol–gel imprint lithography for guided-mode resonance structures

International Nuclear Information System (INIS)

Huang, Yin; Liu, Longju; Lu, Meng; Johnson, Michael; C Hillier, Andrew

2016-01-01

Guided-mode resonance (GMR) structures consisting of sub-wavelength periodic gratings are capable of producing narrow-linewidth optical resonances. This paper describes a sol–gel-based imprint lithography method for the fabrication of submicron 1D and 2D GMR structures. This method utilizes a patterned polydimethylsiloxane (PDMS) mold to fabricate the grating coupler and waveguide for a GMR device using a sol–gel thin film in a single step. An organic–inorganic hybrid sol–gel film was selected as the imprint material because of its relatively high refractive index. The optical responses of several sol–gel GMR devices were characterized, and the experimental results were in good agreement with the results of electromagnetic simulations. The influence of processing parameters was investigated in order to determine how finely the spectral response and resonant wavelength of the GMR devices could be tuned. As an example potential application, refractometric sensing experiments were performed using a 1D sol–gel device. The results demonstrated a refractive index sensitivity of 50 nm/refractive index unit. This one-step fabrication process offers a simple, rapid, and low-cost means of fabricating GMR structures. We anticipate that this method can be valuable in the development of various GMR-based devices as it can readily enable the fabrication of complex shapes and allow the doping of optically active materials into sol–gel thin film. (paper)

Multifunctionalized polyethyleneimine-based nanocarriers for gene and chemotherapeutic drug combination therapy through one-step assembly strategy

Directory of Open Access Journals (Sweden)

Jiang D

2017-12-01

Full Text Available Dandan Jiang,1,* Mingfang Wang,1,* Tianqi Wang,1 Bo Zhang,1 Chunxi Liu,2 Na Zhang1 1Department of Pharmaceutics, Key Laboratory of Chemical Biology (Ministry of Education, School of Pharmaceutical Sciences, Shandong University, Jinan, China; 2Pharmaceutical Department, Qilu Hospital of Shandong University, Jinan, China *These authors contributed equally to this work Abstract: Gene therapy combined with chemotherapy to achieve synergistic therapeutic effects has been a hot topic in recent years. In this project, the human tumor necrosis factor-related apoptosis-inducing ligand-encoding plasmid gene (TRAIL and doxorubicin (Dox-coloaded multifunctional nanocarrier was constructed based on the theory of circulation, accumulation, internalization, and release. Briefly, polyethyleneimine (PEI was selected as skeleton material to synthesize PEI–polyethylene glycol (PEG–TAT (PPT. Dox was conjugated to PEI using C6-succinimidyl 6-hydrazinonicotinate acetone hydrazone (C6-SANH, and a pH-sensitive Dox-PEI (DP conjugate was obtained. Then, intracellular cationic pH-sensitive cellular assistant PPT and DP were mixed to condense TRAIL, and TRAIL–Dox coloaded PPT/DP/TRAIL (PDT nanocarriers were obtained by one-step assembly. TRAIL was completely condensed by DP or PPT when mass ratios (DP/PPT to TRAIL were up to 100:64, which indicated that DP and PPT could be mixed at any ratio for TRAIL condensation. The intracellular uptake rate of PDT was enhanced (P<0.05 when the contents of PPT in PPT+DP increased from 0 to 30%. Free Dox and TRAIL-loaded nanocarriers (PPT/C6-SANH-PEI/TRAIL [PCT] were selected as controls to verify the synergistic antitumor effects of PDT. Compared with free TRAIL, TRAIL-protein expression was upregulated by PDT and PCT on Western blotting assays. The in vitro cytotoxicity of PDT was significantly enhanced compared to free Dox and PCT (P<0.01. Furthermore, murine PDT nanocarriers showed higher in vivo antitumor ability than both the

Simple and Green Fabrication of a Superhydrophobic Surface by One-Step Immersion for Continuous Oil/Water Separation.

Science.gov (United States)

Zhu, Jingfang; Liu, Bin; Li, Longyang; Zeng, Zhixiang; Zhao, Wenjie; Wang, Gang; Guan, Xiaoyan

2016-07-21

In this paper, stainless steel meshes with superhydrophobic and superoleophilic surfaces were fabricated by rapid and simple one-step immersion in a solution containing hydrochloric acid and stearic acid. The apparent contact angles were tested by a video contact angle measurement system (CA). Field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS) were conducted to characterize the surface topographies and chemical compositions. The SEM results showed that mesh surfaces were covered by ferric stearate (Fe[CH3(CH2)16COO]2) with low surface energy. The CA test results showed that the mesh had a maximum apparent contact angle of 160 ± 1.0° and a sliding angle of less than 5.0° for the water droplet, whereas the apparent contact angle for the oil droplet was zero. Ultrasound oscillation and exposure tests at atmospheric conditions and immersion tests in 3.5 wt % NaCl aqueous solution were conducted to confirm the mesh with excellent superhydrophobic and superoleophilic properties. On the basis of the superhydrophobic mesh, a miniature separation device pump was designed to collect pure oil from the oil/water mixture. It showed that the device was easier and convenient. The techniques and materials presented in this work are promising for application to wastewater and oil spill treatment.

Blue-green luminescent CdZnSeS nanocrystals synthesized with activated alkyl thiol

International Nuclear Information System (INIS)

Xia Xing; Liu Zuli; Du Guihuan; Li Yuebin; Ma Ming; Yao Kailun

2012-01-01

Semiconductor nanocrystals with blue-green luminescence are potentially useful in various applications, but the preparation has not been easy compared to regular semiconductor nanocrystals with emission in the orange-red range. In this research alloyed CdZnSeS nanocrystals with luminescence covering the wavelength range from 430 to 560 nm are obtained by a one-step method with the assistance of alkyl thiol compound 1-dodecanethiol, which serves both as the sulfur source and surface ligand. The luminescence of CdZnSeS nanocrystals can be tuned from blue to green by altering the Cd:Zn molar ratio. Besides, the amount of 1-dodecanethiol in the reaction mixture can influence the emission wavelength by restricting the growth of nanocrystals. The dual control of both particle composition and size has enabled the tuning of luminescence to cover the blue-green spectral window. This research presents a convenient method to synthesize nanocrystals with tunable blue-green emission; these materials can be useful in advanced technologies such as photovoltaics, lighting and display. - Highlights: → Obtained blue-green luminescent nanocrystals by a one-step process. → Alkyl thiol used as a sulfur source and a surface stabilizer to control particle size. → Luminescence color of NCs could be easily tuned by changing their composition and particle size simultaneously.

Green technology for conversion of renewable hydrocarbon based on plasma-catalytic approach

Science.gov (United States)

Fedirchyk, Igor; Nedybaliuk, Oleg; Chernyak, Valeriy; Demchina, Valentina

2016-09-01

The ability to convert renewable biomass into fuels and chemicals is one of the most important steps on our path to green technology and sustainable development. However, the complex composition of biomass poses a major problem for established conversion technologies. The high temperature of thermochemical biomass conversion often leads to the appearance of undesirable byproducts and waste. The catalytic conversion has reduced yield and feedstock range. Plasma-catalytic reforming technology opens a new path for biomass conversion by replacing feedstock-specific catalysts with free radicals generated in the plasma. We studied the plasma-catalytic conversion of several renewable hydrocarbons using the air plasma created by rotating gliding discharge. We found that plasma-catalytic hydrocarbon conversion can be conducted at significantly lower temperatures (500 K) than during the thermochemical ( 1000 K) and catalytic (800 K) conversion. By using gas chromatography, we determined conversion products and found that conversion efficiency of plasma-catalytic conversion reaches over 85%. We used obtained data to determine the energy yield of hydrogen in case of plasma-catalytic reforming of ethanol and compared it with other plasma-based hydrogen-generating systems.

Progesterone receptor isoform analysis by quantitative real-time polymerase chain reaction in formalin-fixed, paraffin-embedded canine mammary dysplasias and tumors

DEFF Research Database (Denmark)

Guil-Luna, S.; Stenvang, Jan; Brünner, Nils

2014-01-01

and its isoforms in formalin-fixed, paraffin-embedded tissue samples from canine mammary lesions (4 dysplasias, 10 benign tumors, and 46 carcinomas) using 1-step SYBR Green quantitative real-time polymerase chain reaction (RT-qPCR). Progesterone receptor was expressed in 75% of dysplasias, all benign...... in the expression of isoform A versus B. Analysis of progesterone receptor mRNA isoforms by RT-qPCR was successful in routinely formalin-fixed, paraffin-embedded tissue samples and enabled the distribution of isoforms A and B to be identified for the first time in dysplasias, benign tumors, and malignant tumors...

Infra-red asymptotic behaviour of the one-fermion Green's function in a scalar model with isospin

International Nuclear Information System (INIS)

Popov, V.N.; Wu, T.T.

1979-01-01

In a theory where massive fermions interact with a massless scalar field of isospin 1, the behaviour of the one-fermion Green's function is found to differ from the free Green's function by a factor (1 - (2g 2 /π 2 )ln mmod(x-y))sup(-3/8), in the limit of large separation mod(x-y). (Auth.)

A one-step strategy for ultra-fast and low-cost mass production of plastic membrane microfluidic chips.

Science.gov (United States)

Hu, Chong; Lin, Sheng; Li, Wanbo; Sun, Han; Chen, Yangfan; Chan, Chiu-Wing; Leung, Chung-Hang; Ma, Dik-Lung; Wu, Hongkai; Ren, Kangning

2016-10-05

An ultra-fast, extremely cost-effective, and environmentally friendly method was developed for fabricating flexible microfluidic chips with plastic membranes. With this method, we could fabricate plastic microfluidic chips rapidly (within 12 seconds per piece) at an extremely low cost (less than $0.02 per piece). We used a heated perfluoropolymer perfluoroalkoxy (often called Teflon PFA) solid stamp to press a pile of two pieces of plastic membranes, low density polyethylene (LDPE) and polyethylene terephthalate (PET) coated with an ethylene-vinyl acetate copolymer (EVA). During the short period of contact with the heated PFA stamp, the pressed area of the membranes permanently bonded, while the LDPE membrane spontaneously rose up at the area not pressed, forming microchannels automatically. These two regions were clearly distinguishable even at the micrometer scale so we were able to fabricate microchannels with widths down to 50 microns. This method combines the two steps in the conventional strategy for microchannel fabrication, generating microchannels and sealing channels, into a single step. The production is a green process without using any solvent or generating any waste. Also, the chips showed good resistance against the absorption of Rhodamine 6G, oligonucleotides, and green fluorescent protein (GFP). We demonstrated some typical microfluidic manipulations with the flexible plastic membrane chips, including droplet formation, on-chip capillary electrophoresis, and peristaltic pumping for quantitative injection of samples and reagents. In addition, we demonstrated convenient on-chip detection of lead ions in water samples by a peristaltic-pumping design, as an example of the application of the plastic membrane chips in a resource-limited environment. Due to the high speed and low cost of the fabrication process, this single-step method will facilitate the mass production of microfluidic chips and commercialization of microfluidic technologies.

How Efficient is Green Revolution Technology Adoption in Ghana ...

African Journals Online (AJOL)

The study seeks to find out the effects of Green Revolution technology adoption on output/efficiency of agricultural households in Ghana. The method of analysis involves Battese and Coelli's (1993; 1995) one-step estimation of a stochastic frontier model. Technology adoption was found to have positive effects on output.

Efficacy of SYBR 14/propidium iodide viability stain for the amphibian chytrid fungus Batrachochytrium dendrobatidis.

Science.gov (United States)

Stockwell, M P; Clulow, J; Mahony, M J

2010-01-25

The amphibian chytrid fungus Batrachochytrium dendrobatidis is a recently described pathogen that has been implicated as a causal agent in the global decline in amphibians. Research into its biology and epidemiology has frequently involved in vitro experimentation. However, this research is currently limited by the inability to differentiate between viable and inviable zoospores. Stains are frequently used to determine cell viability, and this study tested a 2-colour fluorescence assay for the detection and quantification of viable B. dendrobatidis zoospores. The results show that the nucleic acid stains SYBR 14 and propidium iodide are effective in distinguishing live from dead zoospores, and a protocol has been optimized for their use. This viability assay provides an efficient and reliable tool that will have applications in B. dendrobatidis challenge and amphibian exposure experiments.

One step gold (bio)functionalisation based on CS{sub 2}-amine reaction

Energy Technology Data Exchange (ETDEWEB)

Almeida, Ines [Centro de Quimica e Bioquimica, Faculdade de Ciencias da Universidade de Lisboa, Ed. C8, Campo Grande, 1749-016 Lisboa (Portugal); Cascalheira, Antonio C. [Lumisense, Lda, Campus Faculdade de Ciencias da Universidade de Lisboa, Ed. ICAT, Campo Grande, 1749-016 Lisboa (Portugal); Viana, Ana S., E-mail: anaviana@fc.ul.p [Centro de Quimica e Bioquimica, Faculdade de Ciencias da Universidade de Lisboa, Ed. C8, Campo Grande, 1749-016 Lisboa (Portugal)

2010-12-01

Dithiocarbamates have been regarded as alternative anchor groups to thiols on gold surfaces, and claimed to be formed in situ through the reaction between secondary amines and carbon disulphide. In this paper, we further exploit this methodology for a convenient one step biomolecule immobilisation onto gold surfaces. First, the reactivity between CS{sub 2} and electroactive compounds containing amines, primary (dopamine), secondary (epinephrine), and an amino acid (tryptophan) has been investigated by electrochemical methods. Cyclic voltammetric characterisation of the modified electrodes confirmed the immobilisation of all the target compounds, allowing the estimation of their surface concentration. The best result was obtained with epinephrine, a secondary amine, for which a typical quasi-reversible behaviour of surface confined electroactive species could be clearly depicted. Electrochemical reductive desorption studies enabled to infer on the extent of the reaction and on the relative stability of the generated monolayers. Bio-functionalisation studies have been accomplished through the reaction of CS{sub 2} with glucose oxidase in aqueous medium, and the catalytic activity of the immobilised enzyme was evaluated towards glucose, by electrochemical methods in the presence of a redox mediator. Scanning tunnelling microscopy (STM) and Atomic force microscopy (AFM) were used respectively, to characterize the gold electrodes and Glucose Oxidase coverage and distribution on the modified surfaces.

Public Participation Procedure in Integrated Transport and Green Infrastructure Planning

Science.gov (United States)

Finka, Maroš; Ondrejička, Vladimír; Jamečný, Ľubomír; Husár, Milan

2017-10-01

The dialogue among the decision makers and stakeholders is a crucial part of any decision-making processes, particularly in case of integrated transportation planning and planning of green infrastructure where a multitude of actors is present. Although the theory of public participation is well-developed after several decades of research, there is still a lack of practical guidelines due to the specificity of public participation challenges. The paper presents a model of public participation for integrated transport and green infrastructure planning for international project TRANSGREEN covering the area of five European countries - Slovakia, Czech Republic, Austria, Hungary and Romania. The challenge of the project is to coordinate the efforts of public actors and NGOs in international environment in oftentimes precarious projects of transport infrastructure building and developing of green infrastructure. The project aims at developing and environmentally-friendly and safe international transport network. The proposed public participation procedure consists of five main steps - spread of information (passive), collection of information (consultation), intermediate discussion, engagement and partnership (empowerment). The initial spread of information is a process of communicating with the stakeholders, informing and educating them and it is based on their willingness to be informed. The methods used in this stage are public displays, newsletters or press releases. The second step of consultation is based on transacting the opinions of stakeholders to the decision makers. Pools, surveys, public hearings or written responses are examples of the multitude of ways to achieve this objective and the main principle of openness of stakeholders. The third step is intermediate discussion where all sides of are invited to a dialogue using the tools such as public meetings, workshops or urban walks. The fourth step is an engagement based on humble negotiation, arbitration and

Distribution of green open space in Malang City based on multispectral data

Science.gov (United States)

Hasyim, A. W.; Hernawan, F. P.

2017-06-01

Green open space is one of the land that its existence is quite important in urban areas where the minimum area is set to reach 30% of the total area of the city. Malang which has an area of 110,6 square kilometers, is one of the major cities in East Java Province that is prone to over-land conversion due to development needs. In support of the green space program, calculation of green space is needed precisely so that remote sensing which has high accuracy is now used for measurement of green space. This study aims to analyze the area of green open space in Malang by using Landsat 8 image in 2015. The method used was the vegetation index that is Normalized Difference Vegetation Index (NDVI). From the study obtained the calculation of green open space was better to use the vegetation index method to avoid the occurrence of misclassification of other types of land use. The results of the calculation of green open space using NDVI found that the area of green open space in Malang City in 2015 reached 39% of the total area.

One-step liquid phase chemical method to prepare carbon-based amorphous molybdenum sulfides: As the effective hydrogen evolution reaction catalysts

International Nuclear Information System (INIS)

Guo, Mengmeng; Wu, Qikang; Yu, Miaomiao; Wang, Yinling; Li, Maoguo

2017-01-01

Two different kinds of carbon-based amorphous molybdenum sulfide composite catalysts (activated carbon supported amorphous molybdenum sulfide and acetylene black supported amorphous molybdenum sulfide) had been prepared in a facile and scalable one-step liquid phase chemical method. The morphological and structural information of catalysts was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), X-Ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM) and it’s electro-catalytic HER activity were evaluated by linear sweep voltammetry(LSV), amperometric i-t technology and AC impedance technology. The as-prepared carbon-based amorphous molybdenum sulfides showed greatly enhanced electro-catalytic activity for HER compared with pure amorphous molybdenum sulfides. Especially, the nano-sized acetylene black supported molybdenum sulfide exhibited excellent electro-catalytic HER performances with a low onset potential of −116 mV versus reverse hydrogen electrode (RHE) and a small Tafel slope of 51 mV per decade.

Developing agricultural opportunities on mine tailings : the Green Mines green energy initiative

Energy Technology Data Exchange (ETDEWEB)

Tisch, B.; Spiers, G.; Beckett, P.; Lock, A. [Natural Resources Canada, Ottawa, ON (Canada). CANMET Mining and Mineral Sciences Laboratories

2009-02-15

The goal of the Green Mines green energy initiative is to advance mine reclamation through the beneficial use of organic residuals for the sustainable establishment of bioenergy crops and other productive land uses. Target organic residuals include: source separated organic compost; papermill biosolids; leaf and yard waste compost; and municipal wastewater biosolids. This presentation discussed the Green Mines green energy initiative with particular reference to potential uses; current participants; scope of the initiative; and progress to date. The presentation also discussed a column study that involved adding filter, filter fabric, silica sand and polyethylene beads to the base of columns. Unoxidized tailings were slurried and pumped into columns and then the oxidized tailings were dried and homogenized. The results of acidic copper/nickel tailings with lime and no lime were also discussed. A summary of findings from the column study was offered. It was found that nutrient management must be considered and organic covers appear to increase metal and arsenic leaching from unlimed tailings. The presentation also made reference to demonstration field plots; biosolids delivery; tilling; monitoring; biomass sampling; and harvesting. The presentation concluded with a discussion of next steps which involve completing construction of the current suite of field plots and implementing full monitoring. figs.

Evaluation of one-step micro polishers for residual resin removal after debonding on fluorosed teeth

Directory of Open Access Journals (Sweden)

Padmalatha Challa

2014-01-01

Full Text Available Aim and objectives: To evaluate the effectiveness of one step micro polishers for residual resin removal on fluorosed teeth using scanning electron microscope (SEM. Methods and Material: 55 teeth with mild to moderate fluorosis were selected with five teeth as control. Metal brackets were bonded onto 50 teeth which were divided into 5 groups. The finishing and polishing methods which were tested include tungsten carbide burs (TCB, multistep finishing system (Sof-Lex, one step polishers (PoGo and combination of TCB with multistep and one step polishing systems. After resin removal, all the samples were examined under SEM for assessment of the enamel surface. Results: The enamel surface was closest to untouched enamel in samples finished with the PoGo one step polishers followed by Sof-Lex multistep finishing system. However, they took the longest time to finish. TCB required the shortest time for residual resin removal. Conclusions: All polishing systems produce a certain degree of damage to the enamel surface with the smoothest surface being produced by one step polishers on fluorosed teeth.

Picosecond dynamics of conformation changes in malachite green dye produced by photoionization of malachite green leucocyanide

Energy Technology Data Exchange (ETDEWEB)

Cremers, D A; Cremers, T L

1983-01-07

The appearance of malachite green dye absorption following photoionization of malachite green leucocyanide has been examined using picosecond flash photolysis. The rate of absorption increase depends upon solvent viscosity and exhibits a two-step behavior in viscous glycerol solutions. 21 references, 4 figures.

Large-area gold nanohole arrays fabricated by one-step method for surface plasmon resonance biochemical sensing.

Science.gov (United States)

Qi, Huijie; Niu, Lihong; Zhang, Jie; Chen, Jian; Wang, Shujie; Yang, Jingjing; Guo, Siyi; Lawson, Tom; Shi, Bingyang; Song, Chunpeng

2018-04-01

Surface plasmon resonance (SPR) nanosensors based on metallic nanohole arrays have been widely reported to detect binding interactions in biological specimens. A simple and effective method for constructing nanoscale arrays is essential for the development of SPR nanosensors. In this work, we report a one-step method to fabricate nanohole arrays by thermal nanoimprinting in the matrix of IPS (Intermediate Polymer Stamp). No additional etching process or supporting substrate is required. The preparation process is simple, time-saving and compatible for roll-to-roll process, potentially allowing mass production. Moreover, the nanohole arrays were integrated into detection platform as SPR sensors to investigate different types of biological binding interactions. The results demonstrate that our one-step method can be used to efficiently fabricate large-area and uniform nanohole arrays for biochemical sensing.

The symmetric MSD encoder for one-step adder of ternary optical computer

Science.gov (United States)

Kai, Song; LiPing, Yan

2016-08-01

The symmetric Modified Signed-Digit (MSD) encoding is important for achieving the one-step MSD adder of Ternary Optical Computer (TOC). The paper described the symmetric MSD encoding algorithm in detail, and developed its truth table which has nine rows and nine columns. According to the truth table, the state table was developed, and the optical-path structure and circuit-implementation scheme of the symmetric MSD encoder (SME) for one-step adder of TOC were proposed. Finally, a series of experiments were designed and performed. The observed results of the experiments showed that the scheme to implement SME was correct, feasible and efficient.

A Facile One Step Solution Route to Synthesize Cuprous Oxide Nanofluid

Directory of Open Access Journals (Sweden)

Shenoy U. Sandhya

2013-05-01

Full Text Available A cuprous oxide nanofluid stabilized by sodium lauryl sulfate, synthesized by using the one step method, has been reported. Nanofluids were synthesized by using a well‐ controlled surfactant‐assisted solution phase synthesis. The method involved reduction of copper acetate by glucose in a mixture of water and ethylene glycol serving as the base fluid. The synthesized fluid was characterized by X‐ray and electron diffraction techniques, in addition, transmission and field emission microscopic techniques and Fourier transform infra red spectroscopic analysis was undertaken. The rheological property, as well as the thermal conductivity of the fluid, were measured. The variation of reaction parameters considerably affected the size of the particles as well as the reaction rate. The uniform dispersion of the particles in the base fluid led to a stability period of three months under stationary state, augmenting the thermal conductivity of the nanofluid. The method is found to be simple, reliable and fast for the synthesis of Newtonian nanofluids containing cuprous oxide nanoparticles.

From green architecture to architectural green

DEFF Research Database (Denmark)

Earon, Ofri

2011-01-01

that describes the architectural exclusivity of this particular architecture genre. The adjective green expresses architectural qualities differentiating green architecture from none-green architecture. Currently, adding trees and vegetation to the building’s facade is the main architectural characteristics...... they have overshadowed the architectural potential of green architecture. The paper questions how a green space should perform, look like and function. Two examples are chosen to demonstrate thorough integrations between green and space. The examples are public buildings categorized as pavilions. One......The paper investigates the topic of green architecture from an architectural point of view and not an energy point of view. The purpose of the paper is to establish a debate about the architectural language and spatial characteristics of green architecture. In this light, green becomes an adjective...

Development of a high-throughput real time PCR based on a hot-start alternative for Pfu mediated by quantum dots

Science.gov (United States)

Sang, Fuming; Yang, Yang; Yuan, Lin; Ren, Jicun; Zhang, Zhizhou

2015-09-01

Hot start (HS) PCR is an excellent alternative for high-throughput real time PCR due to its ability to prevent nonspecific amplification at low temperature. Development of a cost-effective and simple HS PCR technique to guarantee high-throughput PCR specificity and consistency still remains a great challenge. In this study, we systematically investigated the HS characteristics of QDs triggered in real time PCR with EvaGreen and SYBR Green I dyes by the analysis of amplification curves, standard curves and melting curves. Two different kinds of DNA polymerases, Pfu and Taq, were employed. Here we showed that high specificity and efficiency of real time PCR were obtained in a plasmid DNA and an error-prone two-round PCR assay using QD-based HS PCR, even after an hour preincubation at 50 °C before real time PCR. Moreover, the results obtained by QD-based HS PCR were comparable to a commercial Taq antibody DNA polymerase. However, no obvious HS effect of QDs was found in real time PCR using Taq DNA polymerase. The findings of this study demonstrated that a cost-effective high-throughput real time PCR based on QD triggered HS PCR could be established with high consistency, sensitivity and accuracy.Hot start (HS) PCR is an excellent alternative for high-throughput real time PCR due to its ability to prevent nonspecific amplification at low temperature. Development of a cost-effective and simple HS PCR technique to guarantee high-throughput PCR specificity and consistency still remains a great challenge. In this study, we systematically investigated the HS characteristics of QDs triggered in real time PCR with EvaGreen and SYBR Green I dyes by the analysis of amplification curves, standard curves and melting curves. Two different kinds of DNA polymerases, Pfu and Taq, were employed. Here we showed that high specificity and efficiency of real time PCR were obtained in a plasmid DNA and an error-prone two-round PCR assay using QD-based HS PCR, even after an hour

Semi-Automated Hydrophobic Interaction Chromatography Column Scouting Used in the Two-Step Purification of Recombinant Green Fluorescent Protein

Science.gov (United States)

Murphy, Patrick J. M.

2014-01-01

Background Hydrophobic interaction chromatography (HIC) most commonly requires experimental determination (i.e., scouting) in order to select an optimal chromatographic medium for purifying a given target protein. Neither a two-step purification of untagged green fluorescent protein (GFP) from crude bacterial lysate using sequential HIC and size exclusion chromatography (SEC), nor HIC column scouting elution profiles of GFP, have been previously reported. Methods and Results Bacterial lysate expressing recombinant GFP was sequentially adsorbed to commercially available HIC columns containing butyl, octyl, and phenyl-based HIC ligands coupled to matrices of varying bead size. The lysate was fractionated using a linear ammonium phosphate salt gradient at constant pH. Collected HIC eluate fractions containing retained GFP were then pooled and further purified using high-resolution preparative SEC. Significant differences in presumptive GFP elution profiles were observed using in-line absorption spectrophotometry (A395) and post-run fluorimetry. SDS-PAGE and western blot demonstrated that fluorometric detection was the more accurate indicator of GFP elution in both HIC and SEC purification steps. Comparison of composite HIC column scouting data indicated that a phenyl ligand coupled to a 34 µm matrix produced the highest degree of target protein capture and separation. Conclusions Conducting two-step protein purification using the preferred HIC medium followed by SEC resulted in a final, concentrated product with >98% protein purity. In-line absorbance spectrophotometry was not as precise of an indicator of GFP elution as post-run fluorimetry. These findings demonstrate the importance of utilizing a combination of detection methods when evaluating purification strategies. GFP is a well-characterized model protein, used heavily in educational settings and by researchers with limited protein purification experience, and the data and strategies presented here may aid in

Downstream-based Scheduling for Energy Conservation in Green EPONs

KAUST Repository

Chen, Shen

2012-05-01

Maximizing the optical network unit’s (ONU) sleep time is an effective approach for achieving maximum energy conservation in green Ethernet passive optical networks (EPONs). While overlapping downstream and upstream ONU transmissions can maximize the ONU sleep time, it jeopardizes the quality of service (QoS) performance of the network, especially for downstream traffic in case the overlapping is based on the upstream time slot. In this paper, we study the downstream traffic performance in green EPONs under the limited service discipline and the upstream-based overlapped time window. Specifically, we first derive the expected mean packet delay, and then present a closed-form expression of the ONU sleep time, setting identical upstream/downstream transmission cycle times based on a maximum downstream traffic delay re-quirement. With the proposed system model, we present a novel downstream bandwidth allocation scheme for energy conservation in green EPONs. Simulation results verify the proposed model and highlight the advantages of our scheme over conventional approaches.

In situ biosynthesis of bacterial nanocellulose-CaCO3 hybrid bionanocomposite: One-step process.

Science.gov (United States)

Mohammadkazemi, Faranak; Faria, Marisa; Cordeiro, Nereida

2016-08-01

In this work, a simple and green route to the synthesis of the bacterial nanocellulose-calcium carbonate (BNC/CaCO3) hybrid bionanocomposites using one-step in situ biosynthesis was studied. The CaCO3 was incorporated in the bacterial nanocellulose structure during the cellulose biosynthesis by Gluconacetobacter xylinus PTCC 1734 bacteria. Hestrin-Schramm (HS) and Zhou (Z) culture media were used to the hybrid bionanocomposites production and the effect of ethanol addition was investigated. Attenuated total reflection Fourier transform infrared spectroscopy, field emission scanning electron microscopy, X-ray diffraction, energy-dispersive X-ray spectroscopy, inverse gas chromatography and thermogravimetric analysis were used to characterize the samples. The experimental results demonstrated that the ethanol and culture medium play an important role in the BNC/CaCO3 hybrid bionanocomposites production, structure and properties. The BNC/CaCO3 biosynthesized in Z culture medium revealed higher O/C ratio and amphoteric surface character, which justify the highest CaCO3 content incorporation. The CaCO3 was incorporated into the cellulosic matrix decreasing the bacterial nanocellulose crystallinity. This work reveals the high potential of in situ biosynthesis of BNC/CaCO3 hybrid bionanocomposites and opens a new way to the high value-added applications of bacterial nanocellulose. Copyright © 2016 Elsevier B.V. All rights reserved.

A microeconomic-based framework for green cooperative communications

KAUST Repository

Bonello, Nicholas

2011-05-01

The global concern on environmental issues is placing its mark on all sectors of the society, including of course, the wireless telecommunications industry. In this paper, we develop a microeconomic-based green radio framework in which a network operator shifts some of its responsibilities towards the intended destination to one of these inactive end-users with the specific intention of reducing the transmitted power of its source nodes and the incurred costs. Our results disclose up to 70% energy savings and up to 65% cost savings over the direct transmission benchmarker, when selecting from approximately 50 eligible relays in the scenario portrayed by the UMTS Rel. 99 standard. © 2011 IEEE.

A microeconomic-based framework for green cooperative communications

KAUST Repository

Bonello, Nicholas; Aissa, Sonia

2011-01-01

The global concern on environmental issues is placing its mark on all sectors of the society, including of course, the wireless telecommunications industry. In this paper, we develop a microeconomic-based green radio framework in which a network operator shifts some of its responsibilities towards the intended destination to one of these inactive end-users with the specific intention of reducing the transmitted power of its source nodes and the incurred costs. Our results disclose up to 70% energy savings and up to 65% cost savings over the direct transmission benchmarker, when selecting from approximately 50 eligible relays in the scenario portrayed by the UMTS Rel. 99 standard. © 2011 IEEE.

Dynamic analysis of the urban-based low-carbon policy using system dynamics: Focused on housing and green space

Energy Technology Data Exchange (ETDEWEB)

Hong, Taehoon, E-mail: hong7@yonsei.ac.kr [Associate Professor, Department of Architectural Engineering, Yonsei University, Seoul, 120-749 (Korea, Republic of); Kim, Jimin, E-mail: cookie6249@yonsei.ac.kr; Jeong, Kwangbok, E-mail: kbjeong7@yonsei.ac.kr [Research Assistant and Ph.D. Student, Department of Architectural Engineering, Yonsei University, Seoul, 120-749 (Korea, Republic of); Koo, Choongwan, E-mail: cwkoo@yonsei.ac.kr [Postdoctoral Fellow, Department of Architectural Engineering, Yonsei University, Seoul, 120-749 (Korea, Republic of)

2015-02-09

To systematically manage the energy consumption of existing buildings, the government has to enforce greenhouse gas reduction policies. However, most of the policies are not properly executed because they do not consider various factors from the urban level perspective. Therefore, this study aimed to conduct a dynamic analysis of an urban-based low-carbon policy using system dynamics, with a specific focus on housing and green space. This study was conducted in the following steps: (i) establishing the variables of urban-based greenhouse gases (GHGs) emissions; (ii) creating a stock/flow diagram of urban-based GHGs emissions; (iii) conducting an information analysis using the system dynamics; and (iv) proposing the urban-based low-carbon policy. If a combined energy policy that uses the housing sector (30%) and the green space sector (30%) at the same time is implemented, 2020 CO{sub 2} emissions will be 7.23 million tons (i.e., 30.48% below 2020 business-as-usual), achieving the national carbon emissions reduction target (26.9%). The results of this study could contribute to managing and improving the fundamentals of the urban-based low-carbon policies to reduce greenhouse gas emissions.

Dynamic analysis of the urban-based low-carbon policy using system dynamics: Focused on housing and green space

International Nuclear Information System (INIS)

Hong, Taehoon; Kim, Jimin; Jeong, Kwangbok; Koo, Choongwan

2015-01-01

To systematically manage the energy consumption of existing buildings, the government has to enforce greenhouse gas reduction policies. However, most of the policies are not properly executed because they do not consider various factors from the urban level perspective. Therefore, this study aimed to conduct a dynamic analysis of an urban-based low-carbon policy using system dynamics, with a specific focus on housing and green space. This study was conducted in the following steps: (i) establishing the variables of urban-based greenhouse gases (GHGs) emissions; (ii) creating a stock/flow diagram of urban-based GHGs emissions; (iii) conducting an information analysis using the system dynamics; and (iv) proposing the urban-based low-carbon policy. If a combined energy policy that uses the housing sector (30%) and the green space sector (30%) at the same time is implemented, 2020 CO 2 emissions will be 7.23 million tons (i.e., 30.48% below 2020 business-as-usual), achieving the national carbon emissions reduction target (26.9%). The results of this study could contribute to managing and improving the fundamentals of the urban-based low-carbon policies to reduce greenhouse gas emissions

The green building envelope : Vertical greening

NARCIS (Netherlands)

Ottelé, M.

2011-01-01

Planting on roofs and façades is one of the most innovative and fastest developing fields of green technologies with respect to the built environment and horticulture. This thesis is focused on vertical greening of structures and to the multi-scale benefits of vegetation. Vertical green can improve

Financial analysis of community-based forest enterprises with the Green Value tool

Science.gov (United States)

S. Humphries; Tom Holmes

2016-01-01

The Green Value tool was developed in response to the need for simplified procedures that could be used in the field to conduct financial analysis for community-based forest enterprises (CFEs). Initially our efforts focused on a set of worksheets that could be used by both researchers and CFEs to monitor and analyze costs and income for one production period. The...

Accelerated solvent extraction method with one-step clean-up for hydrocarbons in soil

International Nuclear Information System (INIS)

Nurul Huda Mamat Ghani; Norashikin Sain; Rozita Osman; Zuraidah Abdullah Munir

2007-01-01

The application of accelerated solvent extraction (ASE) using hexane combined with neutral silica gel and sulfuric acid/ silica gel (SA/ SG) to remove impurities prior to analysis by gas chromatograph with flame ionization detector (GC-FID) was studied. The efficiency of extraction was evaluated based on the three hydrocarbons; dodecane, tetradecane and pentadecane spiked to soil sample. The effect of ASE operating conditions (extraction temperature, extraction pressure, static time) was evaluated and the optimized condition obtained from the study was extraction temperature of 160 degree Celsius, extraction pressure of 2000 psi with 5 minutes static extraction time. The developed ASE with one-step clean-up method was applied in the extraction of hydrocarbons from spiked soil and the amount extracted was comparable to ASE extraction without clean-up step with the advantage of obtaining cleaner extract with reduced interferences. Therefore in the developed method, extraction and clean-up for hydrocarbons in soil can be achieved rapidly and efficiently with reduced solvent usage. (author)

Validation of a One-Step Method for Extracting Fatty Acids from Salmon, Chicken and Beef Samples.

Science.gov (United States)

Zhang, Zhichao; Richardson, Christine E; Hennebelle, Marie; Taha, Ameer Y

2017-10-01

Fatty acid extraction methods are time-consuming and expensive because they involve multiple steps and copious amounts of extraction solvents. In an effort to streamline the fatty acid extraction process, this study compared the standard Folch lipid extraction method to a one-step method involving a column that selectively elutes the lipid phase. The methods were tested on raw beef, salmon, and chicken. Compared to the standard Folch method, the one-step extraction process generally yielded statistically insignificant differences in chicken and salmon fatty acid concentrations, percent composition and weight percent. Initial testing showed that beef stearic, oleic and total fatty acid concentrations were significantly lower by 9-11% with the one-step method as compared to the Folch method, but retesting on a different batch of samples showed a significant 4-8% increase in several omega-3 and omega-6 fatty acid concentrations with the one-step method relative to the Folch. Overall, the findings reflect the utility of a one-step extraction method for routine and rapid monitoring of fatty acids in chicken and salmon. Inconsistencies in beef concentrations, although minor (within 11%), may be due to matrix effects. A one-step fatty acid extraction method has broad applications for rapidly and routinely monitoring fatty acids in the food supply and formulating controlled dietary interventions. © 2017 Institute of Food Technologists®.

High-Capacity Hydrogen-Based Green-Energy Storage Solutions For The Grid Balancing

Science.gov (United States)

D'Errico, F.; Screnci, A.

One of the current main challenges in green-power storage and smart grids is the lack of effective solutions for accommodating the unbalance between renewable energy sources, that offer intermittent electricity supply, and a variable electricity demand. Energy management systems have to be foreseen for the near future, while they still represent a major challenge. Integrating intermittent renewable energy sources, by safe and cost-effective energy storage systems based on solid state hydrogen is today achievable thanks to recently some technology breakthroughs. Optimized solid storage method made of magnesium-based hydrides guarantees a very rapid absorption and desorption kinetics. Coupled with electrolyzer technology, high-capacity storage of green-hydrogen is therefore practicable. Besides these aspects, magnesium has been emerging as environmentally friend energy storage method to sustain integration, monitoring and control of large quantity of GWh from high capacity renewable generation in the EU.

Design and Development of Low P-Emission Substrate for the Protection of Urban Water Bodies Collecting Green Roof Runoff

Directory of Open Access Journals (Sweden)

Agnieszka Karczmarczyk

2017-10-01

Full Text Available Urbanization leads to higher phosphorus (P concentration in urban catchments. Among different stormwater retention measures, green roofs are the least efficient in phosphorus retention. Moreover, much research has shown that green roofs act as sources of phosphorus, and they can emit P in significant loads. In this study low P emission green roof substrate was developed based on the proposed step by step procedure for the selection of materials including laboratory tests, column experiments, and the monitoring of the open air green roof model. Developed substrate is the mixture of crushed red brick (35% of volume, crushed limestone (20% of volume, and sand (45% of volume, and is characterized by a bulk density of 1.52 g/cm3, water permeability of 9 mm/min, water capacity of 24.6% of volume, and granulometric composition that meets the Landscaping and Landscape Development Research Society (FLL guidelines. Limestone was added to limit the potential P leaching from crushed red brick and vegetated mate consisted of Sedum album, Sedum acre, Sedum kamtschaticum, Sedum spurium, Sedum reflexum, Sedum sexangulare, Dianthus deltoides, Dianthus carthusianorum, and Thymus vulgaris. The open air model experiment was run for 319 days, from March 2015 to February 2016. The total water runoff from the green roof model amounted to 43.3% of runoff from the reference roof. The only one runoff event polluted with phosphorus was connected with the outflow of melted snow from an unfreezing green roof model.

The bounded proof property via step algebras and step frames

NARCIS (Netherlands)

Bezhanishvili, N.; Ghilardi, Silvio

2013-01-01

We develop a semantic criterion for a specific rule-based calculus Ax axiomatizing a given logic L to have the so-called bounded proof property. This property is a kind of an analytic subformula property limiting the proof search space. Our main tools are one-step frames and one-step algebras. These

One-step, green, and economic synthesis of water-soluble photoluminescent carbon dots by hydrothermal treatment of wheat straw, and their bio-applications in labeling, imaging, and sensing

Energy Technology Data Exchange (ETDEWEB)

Yuan, Ming; Zhong, Ruibo; Gao, Haiyang; Li, Wanrong; Yun, Xiaoling; Liu, Jingran; Zhao, Xinmin; Zhao, Guofen; Zhang, Feng, E-mail: fengzhang1978@hotmail.com

2015-11-15

Graphical abstract: Water-soluble photoluminescent carbon dots can be synthesized simply by a green, economic and one-pot hydrothermal treatment of wheat straw with ∼20% yield, in addition to the compact size and robust photostability they are experimentally demonstrated for multiplexed applications such as sensing ions and labeling and imaging for inorganic nanostructures, cells and even nematodes. The converting biomass wastes to promising biocompatible nanomaterials could be a “one-stone-two-birds” strategy to other carbon-containing biomass waste for a highly effectively carbon recycling use and sustainable energy and environment future. - Highlights: • Photoluminescent carbon dots can be synthesized by wheat straw with about 20% yield. • Carbon dots can be used for both nonliving and living labeling, imaging, and sensing. • Carbon dots can be used as a fluorescent ink. - Abstract: The use of biomass as renewable and sustainable energy source has attracted the attention of politics and research and development (R&D) facilities around the world. Agricultural straw acts as a typical biowaste, which still needs highly effective recycling to save the biomass urgently at present. Photoluminescent carbon dots (C-dots) are novel biocompatible nanomaterials that have been proved to be produced from many carbon-abundant materials and hold great promise for the modern nanobiomedicine. In order to realize a “one-stone-two-birds” strategy, we report a green, economic, one-pot method in this article for synthesizing photoluminescent C-dots by hydrothermal treatment of wheat straw. Using X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), we show that the as-prepared C-dots are amorphous in structure and are mainly composed of carbon. Their tiny size (<2 nm), combined with the characteristic excitation-dependent relatively bright emission, and robust photostability made the C-dots a potential biocompatible nanomaterial for bio-applications. We

One-step fabrication of submicrostructures by low one-photon absorption direct laser writing technique with local thermal effect

Science.gov (United States)

Nguyen, Dam Thuy Trang; Tong, Quang Cong; Ledoux-Rak, Isabelle; Lai, Ngoc Diep

2016-01-01

In this work, local thermal effect induced by a continuous-wave laser has been investigated and exploited to optimize the low one-photon absorption (LOPA) direct laser writing (DLW) technique for fabrication of polymer-based microstructures. It was demonstrated that the temperature of excited SU8 photoresist at the focusing area increases to above 100 °C due to high excitation intensity and becomes stable at that temperature thanks to the use of a continuous-wave laser at 532 nm-wavelength. This optically induced thermal effect immediately completes the crosslinking process at the photopolymerized region, allowing obtain desired structures without using the conventional post-exposure bake (PEB) step, which is usually realized after the exposure. Theoretical calculation of the temperature distribution induced by local optical excitation using finite element method confirmed the experimental results. LOPA-based DLW technique combined with optically induced thermal effect (local PEB) shows great advantages over the traditional PEB, such as simple, short fabrication time, high resolution. In particular, it allowed the overcoming of the accumulation effect inherently existed in optical lithography by one-photon absorption process, resulting in small and uniform structures with very short lattice constant.

Effect of bur-cut dentin on bond strength using two all-in-one and one two-step adhesive systems.

Science.gov (United States)

Koase, Kaori; Inoue, Satoshi; Noda, Mamoru; Tanaka, Toru; Kawamoto, Chiharu; Takahashi, Akiko; Nakaoki, Yasuko; Sano, Hidehiko

2004-01-01

To compare the microtensile bond strength (MTBS) of two all-in-one adhesive systems and one experimental two-step self-etching adhesive system to two types of bur-cut dentin. Using one of the three adhesives, Xeno CF Bond (Xeno), Prompt L-Pop (PL), or the experimental two-step system ABF (ABF), resin composite was bonded to flat buccal and root dentin surfaces of eight extracted human premolars. These surfaces were produced using either regular-grit or superfine-grit diamond burs. After storage overnight in 37 degrees C water, the bonded specimens were sectioned into six or seven slices approximately 0.7 mm thick perpendicular to the bonded surface. They were then subjected to microtensile testing. The surfaces of the fractured specimens were observed microscopically to determine the failure mode. In addition, to observe the effect of conditioning, the two types of bur-cut dentin surfaces were conditioned with the adhesives, rinsed with acetone, and observed with SEM. When Xeno and PL were bonded to dentin cut with a regular-grit diamond bur, MTBS values were lower than to superfine bur-cut dentin, and failures occurred adhesively at the interface, whereas the experimental two-step adhesive showed no significant difference in microtensile bond strength between two differently cut surfaces. The all-in-one adhesives tested here improved bond strengths when bonded to superfine bur-cut dentin as a substrate, whereas the experimental two-step adhesive system showed unchanged bonding to both regular and superfine bur-cut dentin surfaces.

Green Clustering Implementation Based on DPS-MOPSO

Directory of Open Access Journals (Sweden)

Yang Lu

2014-01-01

Full Text Available A green clustering implementation is proposed to be as the first method in the framework of an energy-efficient strategy for centralized enterprise high-density WLANs. Traditionally, to maintain the network coverage, all of the APs within the WLAN have to be powered on. Nevertheless, the new algorithm can power off a large proportion of APs while the coverage is maintained as the always-on counterpart. The proposed algorithm is composed of two parallel and concurrent procedures, which are the faster procedure based on K-means and the more accurate procedure based on Dynamic Population Size Multiple Objective Particle Swarm Optimization (DPS-MOPSO. To implement green clustering efficiently and accurately, dynamic population size and mutational operators are introduced as complements for the classical MOPSO. In addition to the function of AP selection, the new green clustering algorithm has another new function as the reference and guidance for AP deployment. This paper also presents simulations in scenarios modeled with ray-tracing method and FDTD technique, and the results show that about 67% up to 90% of energy consumption can be saved while the original network coverage is maintained during periods when few users are online or when the traffic load is low.

One-step preparation of antimicrobial silver nanoparticles in polymer matrix

Energy Technology Data Exchange (ETDEWEB)

Lyutakov, O., E-mail: lyutakoo@vscht.cz; Kalachyova, Y. [Institute of Chemical Technology, Department of Solid State Engineering (Czech Republic); Solovyev, A. [Institute of Chemical Process Fundamentals of the ASCR (Czech Republic); Vytykacova, S. [Institute of Chemical Technology, Department of Glass and Ceramics (Czech Republic); Svanda, J.; Siegel, J. [Institute of Chemical Technology, Department of Solid State Engineering (Czech Republic); Ulbrich, P. [Institute of Chemical Technology, Department of Biochemistry and Microbiology (Czech Republic); Svorcik, V. [Institute of Chemical Technology, Department of Solid State Engineering (Czech Republic)

2015-03-15

Simple one-step procedure for in situ preparation of silver nanoparticles (AgNPs) in the polymer thin films is described. Nanoparticles (NPs) were prepared by reaction of N-methyl pyrrolidone with silver salt in semi-dry polymer film and characterized by transmission electron microscopy, XPS, and UV–Vis spectroscopy techniques. Direct synthesis of NPs in polymer has several advantages; even though it avoids time-consuming NPs mixing with polymer matrix, uniform silver distribution in polymethylmethacrylate (PMMA) films is achieved without necessity of additional stabilization. The influence of the silver concentration, reaction temperature and time on reaction conversion rate, and the size and size-distribution of the AgNPs was investigated. Polymer films doped with AgNPs were tested for their antibacterial activity on Gram-negative bacteria. Antimicrobial properties of AgNPs/PMMA films were found to be depended on NPs concentration, their size and distribution. Proposed one-step synthesis of functional polymer containing AgNPs is environmentally friendly, experimentally simple and extremely quick. It opens up new possibilities in development of antimicrobial coatings with medical and sanitation applications.

One-step preparation of antimicrobial silver nanoparticles in polymer matrix

International Nuclear Information System (INIS)

Lyutakov, O.; Kalachyova, Y.; Solovyev, A.; Vytykacova, S.; Svanda, J.; Siegel, J.; Ulbrich, P.; Svorcik, V.

2015-01-01

Simple one-step procedure for in situ preparation of silver nanoparticles (AgNPs) in the polymer thin films is described. Nanoparticles (NPs) were prepared by reaction of N-methyl pyrrolidone with silver salt in semi-dry polymer film and characterized by transmission electron microscopy, XPS, and UV–Vis spectroscopy techniques. Direct synthesis of NPs in polymer has several advantages; even though it avoids time-consuming NPs mixing with polymer matrix, uniform silver distribution in polymethylmethacrylate (PMMA) films is achieved without necessity of additional stabilization. The influence of the silver concentration, reaction temperature and time on reaction conversion rate, and the size and size-distribution of the AgNPs was investigated. Polymer films doped with AgNPs were tested for their antibacterial activity on Gram-negative bacteria. Antimicrobial properties of AgNPs/PMMA films were found to be depended on NPs concentration, their size and distribution. Proposed one-step synthesis of functional polymer containing AgNPs is environmentally friendly, experimentally simple and extremely quick. It opens up new possibilities in development of antimicrobial coatings with medical and sanitation applications

One step synthesis of 6-oxo-cholestan-3β,5α-diol

International Nuclear Information System (INIS)

Voisin, Maud; Silvente-Poirot, Sandrine; Poirot, Marc

2014-01-01

Highlights: • Cholesterol-5,6-epoxides are metabolized into cholestane-3β,5α,6β-triol (CT) in cancer cells. • 6-Oxo-cholestan-3β,5α-diol (OCDO) is a putative metabolite of CT. • The one step syntheses of CT and OCDO from cholesterol are reported. • The one step syntheses of labelled CT and OCDO are reported. - Abstract: Cholesterol metabolism has been recently linked to cancer, highlighting the importance of the characterization of new metabolic pathways in the sterol series. One of these pathways is centered on cholesterol-5,6-epoxides (5,6-ECs). 5,6-ECs can either generate dendrogenin A, a tumor suppressor present in healthy mammalian tissues, or the carcinogenic cholestane-3β,5α,6β-triol (CT) and its putative metabolite 6-oxo-cholestan-3β,5α-diol (OCDO) in tumor cells. We are currently investigating the identification of the enzyme involved in OCDO biosynthesis, which would be highly facilitated by the use of commercially unavailable [ 14 C]-cholestane-3β,5α,6β-triol and [ 14 C]-6-oxo-cholestan-3β,5α-diol. In the present study we report the one-step synthesis of [ 14 C]-cholestane-3β,5α,6β-triol and [ 14 C]-6-oxo-cholestan-3β,5α-diol by oxidation of [ 14 C]-cholesterol with iodide metaperiodate (HIO 4 )

Production of biovanillin by one-step biotransformation using fungus Pycnoporous cinnabarinus.

Science.gov (United States)

Tilay, Ashwini; Bule, Mahesh; Annapure, Uday

2010-04-14

The current study proposes a one-step biotransformation process for vanillin production from ferulic acid using the wild fungal strain Pycnoporous cinnabarinus belonging to the family Basidiomycete. Improvement of biotransformation conditions was performed in two steps; initially a one factor at a time method was used to investigate effects of medium composition variables (i.e., carbon, nitrogen) and environmental factors such as pH on vanillin production. Subsequently, concentrations of medium components were optimized using an orthogonal matrix method. After primary screening, glucose as carbon source and corn steep liquor and ammonium chloride as organic and inorganic nitrogen source, respectively, supported maximum biotransformation of ferulic acid to vanillin. Under statistically optimum conditions vanillin production from ferulic acid by P. cinnabarinus was 126 mg/L with a molar yield of 54%. The overall molar yield of vanillin production increased by 4 times.

Contribution Index Based on Green Building Certification Systems

Directory of Open Access Journals (Sweden)

Yuting Sun

2015-05-01

Full Text Available Green Building Certification Systems (GBCS are carried out in many countries due to the rising awareness of the importance of sustainability in the building industry. The intention should have motivated participants to construct and operate buildings sustainably, however, there is not yet a method developed to investigate the motivation of the participants. Based on the GBCS, this paper proposes the contribution index as a standard global method to analyze the performance of participants in the green building industry. Three contribution indices, namely Frequency Contribution Index (FCI, Intensity Contribution Index (ICI and Comprehensive Contribution Index (CCI that concern each different category of participant, have been formulated. Three further analyses based on the index were undertaken to investigate some features of the industry. A case study of Singapore was conducted to show how the contribution index could be used to extract industry patterns and trends and assess the participants’ performance in the green building industry. Interviews with experts provide some suggested applications and support for the findings.

Evidence-based practice, step by step: critical appraisal of the evidence: part II: digging deeper--examining the "keeper" studies.

Science.gov (United States)

Fineout-Overholt, Ellen; Melnyk, Bernadette Mazurek; Stillwell, Susan B; Williamson, Kathleen M

2010-09-01

This is the sixth article in a series from the Arizona State University College of Nursing and Health Innovation's Center for the Advancement of Evidence-Based Practice. Evidence-based practice (EBP) is a problem-solving approach to the delivery of health care that integrates the best evidence from studies and patient care data with clinician expertise and patient preferences and values. When delivered in a context of caring and in a supportive organizational culture, the highest quality of care and best patient outcomes can be achieved. The purpose of this series is to give nurses the knowledge and skills they need to implement EBP consistently, one step at a time. Articles will appear every two months to allow you time to incorporate information as you work toward implementing EBP at your institution. Also, we've scheduled "Chat with the Authors" calls every few months to provide a direct line to the experts to help you resolve questions. Details about how to participate in the next call will be published with November's Evidence-Based Practice, Step by Step.

One-step green synthesis and characterization of leaf extract-mediated biocompatible silver and gold nanoparticles from Memecylon umbellatum

Science.gov (United States)

Arunachalam, Kantha D; Annamalai, Sathesh Kumar; Hari, Shanmugasundaram

2013-01-01

In this experiment, green-synthesized silver and gold nanoparticles were produced rapidly by treating silver and gold ions with an extract of Memecylon umbellatum leaf. The reaction process was simple and easy to handle, and was monitored using ultraviolet-visible spectroscopy. The effect of the phytochemicals present in M. umbellatum, including saponins, phenolic compounds, phytosterols, and quinones, on formation of stable silver and gold nanoparticles was investigated by Fourier-transform infrared spectroscopy. The morphology and crystalline phase of the nanoparticles were determined by transmission electron microscopy and energy-dispersive x-ray spectroscopy. The results indicate that the saponins, phytosterols, and phenolic compounds present in the plant extract play a major role in formation of silver and gold nanoparticles in their respective ions in solution. The characteristics of the nanoparticles formed suggest application of silver and gold nanoparticles as chemical sensors in the future. Given the simple and eco-friendly approach for synthesis, these nanoparticles could easily be commercialized for large-scale production. PMID:23569372

An Iterative Ensemble Kalman Filter with One-Step-Ahead Smoothing for State-Parameters Estimation of Contaminant Transport Models

KAUST Repository

Gharamti, M. E.

2015-05-11

The ensemble Kalman filter (EnKF) is a popular method for state-parameters estimation of subsurface flow and transport models based on field measurements. The common filtering procedure is to directly update the state and parameters as one single vector, which is known as the Joint-EnKF. In this study, we follow the one-step-ahead smoothing formulation of the filtering problem, to derive a new joint-based EnKF which involves a smoothing step of the state between two successive analysis steps. The new state-parameters estimation scheme is derived in a consistent Bayesian filtering framework and results in separate update steps for the state and the parameters. This new algorithm bears strong resemblance with the Dual-EnKF, but unlike the latter which first propagates the state with the model then updates it with the new observation, the proposed scheme starts by an update step, followed by a model integration step. We exploit this new formulation of the joint filtering problem and propose an efficient model-integration-free iterative procedure on the update step of the parameters only for further improved performances. Numerical experiments are conducted with a two-dimensional synthetic subsurface transport model simulating the migration of a contaminant plume in a heterogenous aquifer domain. Contaminant concentration data are assimilated to estimate both the contaminant state and the hydraulic conductivity field. Assimilation runs are performed under imperfect modeling conditions and various observational scenarios. Simulation results suggest that the proposed scheme efficiently recovers both the contaminant state and the aquifer conductivity, providing more accurate estimates than the standard Joint and Dual EnKFs in all tested scenarios. Iterating on the update step of the new scheme further enhances the proposed filter’s behavior. In term of computational cost, the new Joint-EnKF is almost equivalent to that of the Dual-EnKF, but requires twice more model

Somatic embryogenesis and in-vitro regeneration of rice (Oryza sativa L.) cultivars under one-step and multiple-step salinity stresses

DEFF Research Database (Denmark)

Khattak, Mohammad S. K.; Abiri, Rambod; Valdiani, Alireza

2017-01-01

The present study aimed to examine the effect of one-step and multiple-step salinity stress on the somatic embryogenesis of rice cultivars within the solid and liquid (cell suspension) culture media conditions. Five rice cultivars, including Puteh Perak, Mahsuri, Basmati-370, Nona Bokra and Khari......, and significant morphological changes were observed. In contrast, the multiple-step NaCl treatment of the calli and cell suspensions led to higher growth of the cultures in the presence of NaCl compared to the controls. The solid MS media, containing 3 μM IAA and 40 μM Kinetin performed as the best media...

Pyrosequencing™ : A one-step method for high resolution HLA typing

Directory of Open Access Journals (Sweden)

Marincola Francesco M

2003-11-01

Full Text Available Abstract While the use of high-resolution molecular typing in routine matching of human leukocyte antigens (HLA is expected to improve unrelated donor selection and transplant outcome, the genetic complexity of HLA still makes the current methodology limited and laborious. Pyrosequencing™ is a gel-free, sequencing-by-synthesis method. In a Pyrosequencing reaction, nucleotide incorporation proceeds sequentially along each DNA template at a given nucleotide dispensation order (NDO that is programmed into a pyrosequencer. Here we describe the design of a NDO that generates a pyrogram unique for any given allele or combination of alleles. We present examples of unique pyrograms generated from each of two heterozygous HLA templates, which would otherwise remain cis/trans ambiguous using standard sequencing based typing (SBT method. In addition, we display representative data that demonstrate long read and linear signal generation. These features are prerequisite of high-resolution typing and automated data analysis. In conclusion Pyrosequencing is a one-step method for high resolution DNA typing.

Detection of live Salmonella enterica in fresh-cut vegetables by a TaqMan-based one-step reverse transcription real-time PCR.

Science.gov (United States)

Miao, Y J; Xiong, G T; Bai, M Y; Ge, Y; Wu, Z F

2018-05-01

Fresh-cut produce is at greater risk of Salmonella contamination. Detection and early warning systems play an important role in reducing the dissemination of contaminated products. One-step Reverse Transcription Polymerase Chain Reaction (RT-qPCR) targeting Salmonella tmRNA with or without a 6-h enrichment was evaluated for the detection of Salmonella in fresh-cut vegetables after 6-h storage. LOD of one-step RT-qPCR was 1·0 CFU per ml (about 100 copies tmRNA per ml) by assessed 10-fold serially diluted RNA from 10 6 CFU per ml bacteria culture. Then, one-step RT-qPCR assay was applied to detect viable Salmonella cells in 14 fresh-cut vegetables after 6-h storage. Without enrichment, this assay could detect 10 CFU per g for fresh-cut lettuce, cilantro, spinach, cabbage, Chinese cabbage and bell pepper, and 10 2 CFU per g for other vegetables. With a 6-h enrichment, this assay could detect 10 CFU per g for all fresh-cut vegetables used in this study. Moreover, this assay was able to discriminate viable cells from dead cells. This rapid detection assay may provide potential processing control and early warning method in fresh-cut vegetable processing to strengthen food safety assurance. Significance and Impact of the Study: Fresh-cut produce is at greater risk of Salmonella contamination. Rapid detection methods play an important role in reducing the dissemination of contaminated products. One-step RT-qPCR assay used in this study could detect 10 CFU per g Salmonella for 14 fresh-cut vegetables with a 6-h short enrichment. Moreover, this assay was able to discriminate viable cells from dead cells. This rapid detection assay may provide potential processing control and early warning method in fresh-cut vegetable processing to strengthen food safety assurance. © 2018 The Society for Applied Microbiology.

Synthesis and characterization of sulfate and dodecylbenzenesulfonate intercalated zinc iron layered double hydroxides by one-step coprecipitation route

Science.gov (United States)

Zhang, Hui; Wen, Xing; Wang, Yingxia

2007-05-01

Inorganic sulfate- and organic dodecylbenzenesulfonate (DBS)-intercalated zinc-iron layered double hydroxides (LDHs) materials were prepared by one-step coprecipitation method from a mixed salt solutions containing Zn(II), Fe(II) and Fe(III) salts. The as-prepared samples have been characterized by X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), low-temperature nitrogen adsorption, scanning electron microscopy (SEM), inductively coupled plasma emission spectroscopy (ICP), and Mössbauer spectroscopy (MS). The XRD analyses demonstrate the typical LDH-like layered structural characteristics of both products. The room temperature MS results reveal the characteristics of both the Fe(II) and Fe(III) species for SO 42--containing product, while only the Fe(III) characteristic for DBS-containing one. The combination characterization results and Rietveld analysis illustrate that the SO 42--containing product possesses the Green Rust two (GR2)-like crystal structure with an approximate chemical composition of [Zn 0.435·Fe II0.094·Fe III0.470·(OH) 2]·(SO 42-) 0.235·1.0H 2O, while the DBS-containing one exhibits the common LDH compound-like structure. The contact angle measurement indicates the evident hydrophobic properties of DBS-containing nanocomposite, compared with SO 42--containing product, due to the modification of the internal and external surface of LDHs by the organic hydrophobic chain of DBS.

An infinite-order two-component relativistic Hamiltonian by a simple one-step transformation.

Science.gov (United States)

Ilias, Miroslav; Saue, Trond

2007-02-14

The authors report the implementation of a simple one-step method for obtaining an infinite-order two-component (IOTC) relativistic Hamiltonian using matrix algebra. They apply the IOTC Hamiltonian to calculations of excitation and ionization energies as well as electric and magnetic properties of the radon atom. The results are compared to corresponding calculations using identical basis sets and based on the four-component Dirac-Coulomb Hamiltonian as well as Douglas-Kroll-Hess and zeroth-order regular approximation Hamiltonians, all implemented in the DIRAC program package, thus allowing a comprehensive comparison of relativistic Hamiltonians within the finite basis approximation.

A one-step, real-time PCR assay for rapid detection of rhinovirus.

Science.gov (United States)

Do, Duc H; Laus, Stella; Leber, Amy; Marcon, Mario J; Jordan, Jeanne A; Martin, Judith M; Wadowsky, Robert M

2010-01-01

One-step, real-time PCR assays for rhinovirus have been developed for a limited number of PCR amplification platforms and chemistries, and some exhibit cross-reactivity with genetically similar enteroviruses. We developed a one-step, real-time PCR assay for rhinovirus by using a sequence detection system (Applied Biosystems; Foster City, CA). The primers were designed to amplify a 120-base target in the noncoding region of picornavirus RNA, and a TaqMan (Applied Biosystems) degenerate probe was designed for the specific detection of rhinovirus amplicons. The PCR assay had no cross-reactivity with a panel of 76 nontarget nucleic acids, which included RNAs from 43 enterovirus strains. Excellent lower limits of detection relative to viral culture were observed for the PCR assay by using 38 of 40 rhinovirus reference strains representing different serotypes, which could reproducibly detect rhinovirus serotype 2 in viral transport medium containing 10 to 10,000 TCID(50) (50% tissue culture infectious dose endpoint) units/ml of the virus. However, for rhinovirus serotypes 59 and 69, the PCR assay was less sensitive than culture. Testing of 48 clinical specimens from children with cold-like illnesses for rhinovirus by the PCR and culture assays yielded detection rates of 16.7% and 6.3%, respectively. For a batch of 10 specimens, the entire assay was completed in 4.5 hours. This real-time PCR assay enables detection of many rhinovirus serotypes with the Applied Biosystems reagent-instrument platform.

Green electricity buyer's guide

International Nuclear Information System (INIS)

Kelly, B.; Klein, S.; Olivastri, B.

2002-06-01

The electricity produced in whole or in large part from renewable energy sources like wind, small hydro electricity and solar energy, is generally referred to as green electricity. The authors designed this buyer's guide to assist customers in their understanding of green electricity, as the customers can now choose their electricity supplier. The considerations and steps involved in the purchasse of green electricity are identified, and advice is provided on ways to maximize the benefits from the purchase of green electricity. In Alberta and Ontario, customers have access to a competitive electricity market. The emphasis when developing this guide was placed firmly on the large buyers, as they can have enormous positive influence on the new market for green electricity. The first chapter of the document provides general information on green electricity. In chapter two, the authors explore the opportunity for environmental leadership. Chapter three reviews the basics of green electricity, which provides the link to chapter four dealing with the creation of a policy. Purchasing green electricity is dealt with in Chapter five, and maximizing the benefits of green electricity are examined in Chapter Six. 24 refs., 3 tabs

Building Envelope Thermal Performance Assessment Using Visual Programming and BIM, based on ETTV requirement of Green Mark and GreenRE

Directory of Open Access Journals (Sweden)

Taki Eddine Seghier

2017-09-01

Full Text Available Accomplishment of green building design requirements and the achievement of the targeted credit points under a specific green rating system are known to be a task that is very challenging. Building Information Modeling (BIM design process and tools have already made considerable advancements in green building design and performance analysis. However, Green building design process is still lack of tools and workflows that can provide real-time feedback of building sustainability and rating during the design stage. In this paper, a new workflow of green building design assessment and rating is proposed based on the integration of Visual Programing Language (VPL and BIM. Thus, the aim of this study is to develop a BIM-VPL based tool for building envelope design and assessment support. The focus performance metric in this research is building Envelope Thermal Transfer Value (ETTV which is an Energy Efficiency (EE prerequisite requirement (up to 15 credits in both Green Mark and GreenRE rating systems. The development of the tool begins first by creating a generic integration framework between BIM-VPL functionalities and ETTV requirements. Then, data is extracted from the BIM 3D model and managed using Revit, Excel and Dynamo for visual scripting. A sample project consisting of a hypothetical residential building is run and its envelope ETTV performance and rating score are obtained for the validation of the tool. This tool will support project team in building envelope design and assessment by allowing them to select the most appropriate façade configuration according to its performance efficiency and the green rating. Furthermore, this tool serves as proof of concept that building sustainability rating and compliance checking can be automatically processed through customized workflows developed based on BIM and VPL technologies.

One Step Solution to the Local Tie Vectors of GNSS/SLR in ITRF

Directory of Open Access Journals (Sweden)

MA Xiaping

2018-01-01

Full Text Available This paper proposes a one-step solution of local tie vectors,the solution takes RP (Reference Point and axes offsets of SLR telescope as unknown parameters,GNSS baselines network and conventional terrestrial observation (horizontal,vertical angle and distance are combined in ITRF (International Terrestrial Reference Frame,and multiply constraint conditions are established between RP and observation marks and two axes offsets.Local tie vectors and related covariance matrices of the 3 co-located sites in Beijing,Kunming,Xi'an of CMONOC (Crustal Movement Observation Network of China are solved with the proposed one step solution.The results show that the RMS (root mean square error of local tie vectors is less than 2.0 mm.Besides,the differences are less than 2.0 mm compared with the traditional step by step solution,and the offsets and corresponding RMS between the horizontal axis and vertical axis are 3.8 mm,0.7 mm,3.6 mm and 1.3 mm,1.2 mm,1.3 mm,respectively.

Effect of one-step polishing system on the color stability of nanocomposites.

Science.gov (United States)

Alawjali, S S; Lui, J L

2013-08-01

This study was to compare the effect of three different one-step polishing systems on the color stability of three different types of nanocomposites after immersion in coffee for one day and seven days and determine which nanocomposite material has the best color stability following polishing with each of the one-step polishing system. The nanocomposites tested were Tetric EvoCeram, Grandio and Herculite Précis. A total of 120 discs (40/nanocomposite, 8mm×2mm) were fabricated. Ten specimens for each nanocomposite cured under Mylar strips served as the control. The other specimens were polished with OptraPol, OneGloss and Occlubrush immersed in coffee (Nescafé) up to seven days. Color measurements were made with a spectrophotometer at baseline and after one and seven days. Two way repeated measure ANOVA, two way ANOVA and Bonferroni tests were used for statistical analyses (P<0.05). The immersion time was a significant factor in the discoloration of the nanocomposites. The effect of three one-step polishing systems on the color stability was also significant. The color change values of the materials cured against Mylar strips were the greatest. The lowest mean color change values were from the Occlubrush polished groups. The effect of the three different types of nanocomposite on the color change was significant. The highest color change values were with Tetric EvoCeram groups. The lowest color change values were with Herculite Précis groups. The color change of nanocomposite resins is affected by the type of composite, polishing procedure and the period of immersion in the staining agent. Copyright © 2012 Elsevier Ltd. All rights reserved.

Empirical Green's function analysis: Taking the next step

Science.gov (United States)

Hough, S.E.

1997-01-01

An extension of the empirical Green's function (EGF) method is presented that involves determination of source parameters using standard EGF deconvolution, followed by inversion for a common attenuation parameter for a set of colocated events. Recordings of three or more colocated events can thus be used to constrain a single path attenuation estimate. I apply this method to recordings from the 1995-1996 Ridgecrest, California, earthquake sequence; I analyze four clusters consisting of 13 total events with magnitudes between 2.6 and 4.9. I first obtain corner frequencies, which are used to infer Brune stress drop estimates. I obtain stress drop values of 0.3-53 MPa (with all but one between 0.3 and 11 MPa), with no resolved increase of stress drop with moment. With the corner frequencies constrained, the inferred attenuation parameters are very consistent; they imply an average shear wave quality factor of approximately 20-25 for alluvial sediments within the Indian Wells Valley. Although the resultant spectral fitting (using corner frequency and ??) is good, the residuals are consistent among the clusters analyzed. Their spectral shape is similar to the the theoretical one-dimensional response of a layered low-velocity structure in the valley (an absolute site response cannot be determined by this method, because of an ambiguity between absolute response and source spectral amplitudes). I show that even this subtle site response can significantly bias estimates of corner frequency and ??, if it is ignored in an inversion for only source and path effects. The multiple-EGF method presented in this paper is analogous to a joint inversion for source, path, and site effects; the use of colocated sets of earthquakes appears to offer significant advantages in improving resolution of all three estimates, especially if data are from a single site or sites with similar site response.

Detection of Salmonella in Shellfish Using SYBR Green™ I-Based Real-Time Multiplexed PCR Assay Targeting invA and spvB

KAUST Repository

Gangwar, Maulshree

2012-09-23

A SYBR Green™ I-based real-time multiplexed PCR assay was developed targeting invA and spvB for the detection of Salmonella strains in shellfish after both hns and invA genes were identified in all Salmonella strains. Simultaneously, the 16S rRNA gene was used as a PCR internal amplification control (IAC). All 89 Salmonella strains tested in this study exhibited amplification of invA, whereas only 21 (23. 6 %) were PCR positive for spvB. The sensitivity of detection of all three targeted genes was 1 ng, which is equivalent to approximately 105 colony-forming unit (CFU) of Salmonella enterica. The analysis showed specific PCR products that were identified by reproducible melt temperature profiles (invA, 84. 27 ± 1. 7 °C; spvB, 88. 76 ± 1. 0 °C; and 16S rRNA gene, 87. 16 ± 0. 8 °C). The sensitivity of detection was 10 pg purified DNA (invA) or 105 CFU in 1 mL pure culture of S. enterica ATCC 14028. The above molecular detection method for Salmonella strains was successfully applied to the oyster homogenates (food matrix). An initial inoculum of 106 and 102 CFU Salmonella in 1 ml seeded oyster tissue homogenate was detected by multiplexed PCR for all three genes after 5 and 24 h of enrichment, respectively. Natural oysters isolated from Gulf of Mexico during the winter months exhibited negative PCR amplification results suggesting the absence of Salmonella. In contrast to conventional PCR, real-time multiplex PCR assay developed in this study is rapid and sensitive and will help Interstate Shellfish Sanitation Conference undertake appropriate measures to monitor Salmonella in oysters, thereby preventing disease outbreaks and consequently protecting consumer health. © 2012 Springer Science+Business Media, LLC.

Detection of Salmonella in Shellfish Using SYBR Green™ I-Based Real-Time Multiplexed PCR Assay Targeting invA and spvB

KAUST Repository

Gangwar, Maulshree; Waters, Alicia M.; Bej, Gautam A.; Bej, Asim K.; Mojib, Nazia

2012-01-01

A SYBR Green™ I-based real-time multiplexed PCR assay was developed targeting invA and spvB for the detection of Salmonella strains in shellfish after both hns and invA genes were identified in all Salmonella strains. Simultaneously, the 16S rRNA gene was used as a PCR internal amplification control (IAC). All 89 Salmonella strains tested in this study exhibited amplification of invA, whereas only 21 (23. 6 %) were PCR positive for spvB. The sensitivity of detection of all three targeted genes was 1 ng, which is equivalent to approximately 105 colony-forming unit (CFU) of Salmonella enterica. The analysis showed specific PCR products that were identified by reproducible melt temperature profiles (invA, 84. 27 ± 1. 7 °C; spvB, 88. 76 ± 1. 0 °C; and 16S rRNA gene, 87. 16 ± 0. 8 °C). The sensitivity of detection was 10 pg purified DNA (invA) or 105 CFU in 1 mL pure culture of S. enterica ATCC 14028. The above molecular detection method for Salmonella strains was successfully applied to the oyster homogenates (food matrix). An initial inoculum of 106 and 102 CFU Salmonella in 1 ml seeded oyster tissue homogenate was detected by multiplexed PCR for all three genes after 5 and 24 h of enrichment, respectively. Natural oysters isolated from Gulf of Mexico during the winter months exhibited negative PCR amplification results suggesting the absence of Salmonella. In contrast to conventional PCR, real-time multiplex PCR assay developed in this study is rapid and sensitive and will help Interstate Shellfish Sanitation Conference undertake appropriate measures to monitor Salmonella in oysters, thereby preventing disease outbreaks and consequently protecting consumer health. © 2012 Springer Science+Business Media, LLC.

Biodiesel Production from Wet Spirulina sp. by One-Step Extraction-Transesterification

Directory of Open Access Journals (Sweden)

Surya Pradana Yano

2018-01-01

Full Text Available Microalgae has gained immense interests as the raw material for biofuel production. The lipid content in microalgae can be converted into biodiesel through conventional method which involves separated process of extraction and transesterification. In this study, the production of biodiesel from Spirulina sp. was performed through one-step extraction-transesterification using KOH as base catalyst to simplify the production of biodiesel. The mixture of methanol-hexane was employed as both solvent and reactant in the process. The resulting biodiesel was found to be mainly composed of methyl oleate and methyl palmitate. On the other hand, increasing the reaction temperature and reducing the quantity of methanol in solvent mixture would also increase the yield of biodiesel. The optimum methanol-hexane volumetric ratio and temperature which gave the highest biodiesel yield were 3:7 and 50°C, respectively.

One step synthesis of 6-oxo-cholestan-3β,5α-diol

Energy Technology Data Exchange (ETDEWEB)

Voisin, Maud; Silvente-Poirot, Sandrine; Poirot, Marc, E-mail: marc.poirot@inserm.fr

2014-04-11

Highlights: • Cholesterol-5,6-epoxides are metabolized into cholestane-3β,5α,6β-triol (CT) in cancer cells. • 6-Oxo-cholestan-3β,5α-diol (OCDO) is a putative metabolite of CT. • The one step syntheses of CT and OCDO from cholesterol are reported. • The one step syntheses of labelled CT and OCDO are reported. - Abstract: Cholesterol metabolism has been recently linked to cancer, highlighting the importance of the characterization of new metabolic pathways in the sterol series. One of these pathways is centered on cholesterol-5,6-epoxides (5,6-ECs). 5,6-ECs can either generate dendrogenin A, a tumor suppressor present in healthy mammalian tissues, or the carcinogenic cholestane-3β,5α,6β-triol (CT) and its putative metabolite 6-oxo-cholestan-3β,5α-diol (OCDO) in tumor cells. We are currently investigating the identification of the enzyme involved in OCDO biosynthesis, which would be highly facilitated by the use of commercially unavailable [{sup 14}C]-cholestane-3β,5α,6β-triol and [{sup 14}C]-6-oxo-cholestan-3β,5α-diol. In the present study we report the one-step synthesis of [{sup 14}C]-cholestane-3β,5α,6β-triol and [{sup 14}C]-6-oxo-cholestan-3β,5α-diol by oxidation of [{sup 14}C]-cholesterol with iodide metaperiodate (HIO{sub 4})

Fast-ion energy resolution by one-step reaction gamma-ray spectrometry

DEFF Research Database (Denmark)

Salewski, Mirko; Nocente, M.; Gorini, G.

2016-01-01

The spectral broadening of γ-rays from fusion plasmas can be measured in high-resolution gamma-ray spectrometry (GRS). We derive weight functions that determine the observable velocity space and quantify the velocity-space sensitivity of one-step reaction high-resolution GRS measurements in magne...

Microchip capillary electrophoresis with laser-induced fluorescence combined with one-step duplex reverse-transcription polymerase chain reaction for the rapid detection of Enterovirus 71 and Coxsackievirus A16 in throat swab specimens.

Science.gov (United States)

Jia, Ruan; Chengjun, Sun; Heng, Chen; Chen, Zhou; Yuanqian, Li; Yongxin, Li

2015-07-01

Enterovirus 71 and Coxsackievirus A16 are the main pathogens causing hand-foot-mouth disease. In this paper, microchip capillary electrophoresis with laser-induced fluorescence combined with one-step duplex reverse transcript-polymerase chain reaction has been developed for the detection of Enterovirus 71 and Coxsackievirus A16 in throat swab specimens. The specific reverse transcription-polymerase chain reaction amplicons labeled with SYBR Orange were separated by microchip capillary electrophoresis and detected by laser induced fluorescence detector within 7 min. The intraday and interday relative standard deviation of migration time for DNA Marker was in the range of 1.36-2.94 and 2.78-3.96%, respectively. The detection limits were as low as 2.06 × 10(3) copies/mL for Enterovirus 71 and 5 × 10(3) copies/mL for Coxsackievirus A16. No cross-reactivity was observed with rotavirus, astrovirus, norovirus, and adenovirus, which showed good specificity of the method. This assay was validated using 100 throat swab specimens that were detected by real-time reverse-transcript polymerase chain reaction in parallel and the two methods produced the same results. This study provided a rapid, sensitive and specific method for the detection of Enterovirus 71 and Coxsackievirus A16, which make a contribution to significant time and cost saving for the identification and treatment of patients. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

One-Step Printable Perovskite Films Fabricated under Ambient Conditions for Efficient and Reproducible Solar Cells.

Science.gov (United States)

Jung, Yen-Sook; Hwang, Kyeongil; Heo, Youn-Jung; Kim, Jueng-Eun; Lee, Donmin; Lee, Cheol-Ho; Joh, Han-Ik; Yeo, Jun-Seok; Kim, Dong-Yu

2017-08-23

Despite the potential of roll-to-roll processing for the fabrication of perovskite films, the realization of highly efficient and reproducible perovskite solar cells (PeSCs) through continuous coating techniques and low-temperature processing is still challenging. Here, we demonstrate that efficient and reliable CH 3 NH 3 PbI 3 (MAPbI 3 ) films fabricated by a printing process can be achieved through synergetic effects of binary processing additives, N-cyclohexyl-2-pyrrolidone (CHP) and dimethyl sulfoxide (DMSO). Notably, these perovskite films are deposited from premixed perovskite solutions for facile one-step processing under a room-temperature and ambient atmosphere. The CHP molecules result in the uniform and homogeneous perovskite films even in the one-step slot-die system, which originate from the high boiling point and low vapor pressure of CHP. Meanwhile, the DMSO molecules facilitate the growth of perovskite grains by forming intermediate states with the perovskite precursor molecules. Consequently, fully printed PeSC based on the binary additive system exhibits a high PCE of 12.56% with a high reproducibility.

Fast Simulation of 3-D Surface Flanging and Prediction of the Flanging Lines Based On One-Step Inverse Forming Algorithm

International Nuclear Information System (INIS)

Bao Yidong; Hu Sibo; Lang Zhikui; Hu Ping

2005-01-01

A fast simulation scheme for 3D curved binder flanging and blank shape prediction of sheet metal based on one-step inverse finite element method is proposed, in which the total plasticity theory and proportional loading assumption are used. The scheme can be actually used to simulate 3D flanging with complex curve binder shape, and suitable for simulating any type of flanging model by numerically determining the flanging height and flanging lines. Compared with other methods such as analytic algorithm and blank sheet-cut return method, the prominent advantage of the present scheme is that it can directly predict the location of the 3D flanging lines when simulating the flanging process. Therefore, the prediction time of flanging lines will be obviously decreased. Two typical 3D curve binder flanging including stretch and shrink characters are simulated in the same time by using the present scheme and incremental FE non-inverse algorithm based on incremental plasticity theory, which show the validity and high efficiency of the present scheme

A facile one-step route to RGO/Ni3S2 for high-performance supercapacitors

International Nuclear Information System (INIS)

Zhang, Zhuomin; Zhao, Chongjun; Min, Shudi; Qian, Xiuzhen

2014-01-01

Graphical abstract: - Highlights: • A uniform 3D nest-like nanostructure of RGO/Ni 3 S 2 nanocomposite on Ni foam, in-situ synthesized using a simple, green one-pot hydrothermal approach, exhibits superior capacitive performance (7440 mF cm −2 at 10 mA cm −2 , i.e., 2188.8 F g −1 at 2.9 A g −1 and 1016 F g −1 at 29.0 A g −1 ).Highlights. • RGO/Ni 3 S 2 /NF composite was in-situ synthesized through a one-step hydrothermal process. • No other reagent except Ni foam, GO and S powder was added. • RGO/Ni 3 S 2 , RGO/Ni(OH) 2 , and RGO/Ni 3 S 2 /Ni(OH) 2 can be controllably synthesized. • As-prepared RGO/Ni 3 S 2 /NF nanocomposite exhibits high capability and cyclability. - Abstract: A facile one-step solution-phase route to RGO/Ni 3 S 2 on nickel foam (RGO/Ni 3 S 2 /NF) was presented. The RGO/Ni 3 S 2 /NF (RNS) nanocomposites were hydrothermal-assisted synthesized, in which nickel foam acted as an auxiliary reductant of GO and S, a Ni source of Ni 3 S 2 , and a substrate for composite film. RGO/Ni 3 S 2 /NF composites were characterized by X-ray diffraction (XRD), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscope (FESEM), and transmission electron microscopy (TEM). The electrochemical performances of the supercapacitor with as-synthesized RGO/Ni 3 S 2 /NF (RNS) electrodes are evaluated using cyclic voltammetry (CV), galvanostatic charge-discharge (GCD), and electrochemical impedance spectrometry (EIS) in 2 M KOH aqueous solution. It is found that the RGO/Ni 3 S 2 /NF electrode exhibits superior supercapacitor performance (7440 mF cm −2 at 10 mA cm −2 , i.e., 2188.8 F g −1 at 2.9 A g −1 ), compared with the Ni 3 S 2 /NF electrode (4360 mF cm −2 at 10 mA cm −2 ) and the RGO/Ni(OH) 2 /NF electrode (3400 mF cm −2 at 10 mA cm −2 ) prepared under identical conditions. Both the temperature and sulfur content play important roles in the controlled synthesis of RNS and its

[Identification of green tea brand based on hyperspectra imaging technology].

Science.gov (United States)

Zhang, Hai-Liang; Liu, Xiao-Li; Zhu, Feng-Le; He, Yong

2014-05-01

Hyperspectral imaging technology was developed to identify different brand famous green tea based on PCA information and image information fusion. First 512 spectral images of six brands of famous green tea in the 380 approximately 1 023 nm wavelength range were collected and principal component analysis (PCA) was performed with the goal of selecting two characteristic bands (545 and 611 nm) that could potentially be used for classification system. Then, 12 gray level co-occurrence matrix (GLCM) features (i. e., mean, covariance, homogeneity, energy, contrast, correlation, entropy, inverse gap, contrast, difference from the second-order and autocorrelation) based on the statistical moment were extracted from each characteristic band image. Finally, integration of the 12 texture features and three PCA spectral characteristics for each green tea sample were extracted as the input of LS-SVM. Experimental results showed that discriminating rate was 100% in the prediction set. The receiver operating characteristic curve (ROC) assessment methods were used to evaluate the LS-SVM classification algorithm. Overall results sufficiently demonstrate that hyperspectral imaging technology can be used to perform classification of green tea.

Pressure-driven one-step solid phase-based on-chip sample preparation on a microfabricated plastic device and integration with flow-through polymerase chain reaction (PCR).

Science.gov (United States)

Tran, Hong Hanh; Trinh, Kieu The Loan; Lee, Nae Yoon

2013-10-01

In this study, we fabricate a monolithic poly(methylmethacrylate) (PMMA) microdevice on which solid phase-based DNA preparation and flow-through polymerase chain reaction (PCR) units were functionally integrated for one-step sample preparation and amplification operated by pressure. Chelex resin, which is used as a solid support for DNA preparation, can capture denatured proteins but releases DNA, and the purified DNA can then be used as a template in a subsequent amplification process. Using the PMMA microdevices, DNA was successfully purified from both Escherichia coli and human hair sample, and the plasmid vector inserted in E. coli and the D1S80 locus in human genomic DNA were successfully amplified from on-chip purified E. coli and human hair samples. Furthermore, the integration potential of the proposed sample preparation and flow-through PCR units was successfully demonstrate on a monolithic PMMA microdevice with a seamless flow, which could pave the way for a pressure-driven, simple one-step sample preparation and amplification with greatly decreased manufacture cost and enhanced device disposability. Copyright © 2013 Elsevier B.V. All rights reserved.

One-step preparation of [18F]FPBM for PET imaging of serotonin transporter (SERT) in the brain

International Nuclear Information System (INIS)

Qiao, Hongwen; Zhang, Yan; Wu, Zehui; Zhu, Lin; Choi, Seok Rye; Ploessl, Karl; Kung, Hank F.

2016-01-01

Serotonin transporters (SERT) in the brain play an important role in normal brain function. Selective serotonin reuptake inhibitors such as fluoxetine, sertraline, paroxetine, escitalopram, etc., specifically target SERT binding in the brain. Development of SERT imaging agents may be useful for studying the function of SERT by in vivo imaging. A one-step preparation of [ 18 F]FPBM, 2-(2′-(dimethylamino)methyl)-4′-(3-([ 18 F]fluoropropoxy)phenylthio) benzenamine, for positron emission tomography (PET) imaging of SERT binding in the brain was achieved. An active OTs intermediate, 9, was reacted with [ 18 F]F − /K 222 to produce [ 18 F]FPBM in one step and in high radiochemical yield. This labeling reaction was evaluated and optimized under different temperatures, bases, solvents, and varying amounts of precursor 9. The radiolabeling reaction led to the desired [ 18 F]FPBM in one step and the crude product was purified by HPLC purification to give no-carrier-added [ 18 F]FPBM (radiochemical yield, 24–33%, decay corrected; radiochemical purity > 99%). PET imaging studies in normal monkeys (n = 4) showed fast, pronounced uptakes in the midbrain and thalamus, regions known to be rich in SERT binding sites. A displacement experiment with escitalopram (5 mg/kg iv injection at 30 min after [ 18 F]FPBM injection) showed a rapid and complete reversal of SERT binding, suggesting that binding by [ 18 F]FPBM was highly specific and reversible. A one-step radiolabeling method coupled with HPLC purification for preparation of [ 18 F]FPBM was developed. Imaging studies suggest that it is feasible to use this method to prepare [ 18 F]FPBM for in vivo PET imaging of SERT binding in the brain.

Shear bond strength of one-step self-etch adhesives to dentin : evaluation of NaOCl pretreatment

OpenAIRE

Colombo, Marco; Beltrami, Riccardo; Chiesa, Marco; Poggio, Claudio; Scribante, Andrea

2018-01-01

The aim of this study was to evaluate the influence of dentin pretreatment with NaOCl on shear bond strength of four one-step self-etch adhesives with different pH values. Bovine permanent incisors were used. Four one-step self-etch adhesives were tested: Adper? Easy Bond, Futurabond NR, G-aenial Bond, Clearfil S3 Bond. One two-step self-etch adhesive (Clearfil SE Bond) was used as control. Group 1- no pretreatment; group 2- pretratment with 5,25 % NaOCl; group 3- pretreatment with 37 % H3PO4...

Real-time PCR-based genotyping from whole blood using Taq DNA polymerase and a buffer supplemented with 1,2-propanediol and trehalose

Czech Academy of Sciences Publication Activity Database

Utekal, Pavol; Kocanda, Lukáš; Matoušek, P.; Wagner, P.; Bugajev, Viktor; Dráber, Petr

2015-01-01

Roč. 416, January (2015), s. 178-182 ISSN 0022-1759 R&D Projects: GA ČR(CZ) GBP302/12/G101; GA MPO FR-TI3/067; GA ČR(CZ) GA14-09807S; GA ČR(CZ) GA14-00703S Institutional support: RVO:68378050 Keywords : 1,2-Propanediol * real-time PCR * SYBR Green I * Taq DNA polymerase * trehalose * Unseparated blood Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.858, year: 2015

One-step Fabrication of Cellulose/Graphene Conductive Paper

Institute of Scientific and Technical Information of China (English)

KaiWen Mou; LuMing Yang; HuangWei Xiong; RuiTao Cha

2017-01-01

In this study,a straightforward,one-step wet-end formation process was employed to prepare cellulose/graphene conductive paper for antistatic packing materials.Cationic polyacrylamide was introduced into the cellulose/graphene slurry to improve the graphene loading on the surfaces of the cellulose fibers.The effect of the super calender process on the properties of the cellulose/graphene conductive paper was investigated.When 55 wt％ graphene was added,the volume resistivity of the cellulose/graphene conductive paper was 94.70 Ω·cm,decreasing to 35.46 Ω·cm after the super calender process.The cellulose/graphene conductive paper possessed excellent anti-static ability and could be used as an anti-static material.

Rapid One-Step Selection Method for Generating Nucleic Acid Aptamers: Development of a DNA Aptamer against alpha-Bungarotoxin

DEFF Research Database (Denmark)

Lauridsen, Lasse Holm; Shamaileh, Hadi A.; Edwards, Stacey L.

2012-01-01

Background: Nucleic acids based therapeutic approaches have gained significant interest in recent years towards the development of therapeutics against many diseases. Recently, research on aptamers led to the marketing of Macugen (R), an inhibitor of vascular endothelial growth factor (VEGF......) for the treatment of age related macular degeneration (AMD). Aptamer technology may prove useful as a therapeutic alternative against an array of human maladies. Considering the increased interest in aptamer technology globally that rival antibody mediated therapeutic approaches, a simplified selection, possibly...... in one-step, technique is required for developing aptamers in limited time period. Principal Findings: Herein, we present a simple one-step selection of DNA aptamers against alpha-bungarotoxin. A toxin immobilized glass coverslip was subjected to nucleic acid pool binding and extensive washing followed...

Advancing Our Understanding of the Etiologies and Mutational Landscapes of Basal Like, Luminal A, and Luminal B Breast Cancers

Science.gov (United States)

2016-10-01

5-45, completed under Dr. Li’s supervision at FHCRC: a. Identification of controls, Months 5-45: General population controls with no prior history ...sequencing and analysis will be validated by SYBR Green based QPCR using Page 11 primers designed using Primer 3 to encompass the fusion junction on a...pools of multiplexed PCR products resequenced on Illumina next-generation sequencing equipment, we will screen any recurrently mutated gene appearing

Robust and unobtrusive algorithm based on position independence for step detection

Science.gov (United States)

Qiu, KeCheng; Li, MengYang; Luo, YiHan

2018-04-01

Running is becoming one of the most popular exercises among the people, monitoring steps can help users better understand their running process and improve exercise efficiency. In this paper, we design and implement a robust and unobtrusive algorithm based on position independence for step detection under real environment. It applies Butterworth filter to suppress high frequency interference and then employs the projection based on mathematics to transform system to solve the problem of unknown position of smartphone. Finally, using sliding window to suppress the false peak. The algorithm was tested for eight participants on the Android 7.0 platform. In our experiments, the results show that the proposed algorithm can achieve desired effect in spite of device pose.

Optimization of One-Step In Situ Transesterification Method for Accurate Quantification of EPA in Nannochloropsis gaditana

Directory of Open Access Journals (Sweden)

Yuting Tang

2016-11-01

Full Text Available Microalgae are a valuable source of lipid feedstocks for biodiesel and valuable omega-3 fatty acids. Nannochloropsis gaditana has emerged as a promising producer of eicosapentaenoic acid (EPA due to its fast growth rate and high EPA content. In the present study, the fatty acid profile of Nannochloropsis gaditana was found to be naturally high in EPA and devoid of docosahexaenoic acid (DHA, thereby providing an opportunity to maximize the efficacy of EPA production. Using an optimized one-step in situ transesterification method (methanol:biomass = 90 mL/g; HCl 5% by vol.; 70 °C; 1.5 h, the maximum fatty acid methyl ester (FAME yield of Nannochloropsis gaditana cultivated under rich condition was quantified as 10.04% ± 0.08% by weight with EPA-yields as high as 4.02% ± 0.17% based on dry biomass. The total FAME and EPA yields were 1.58- and 1.23-fold higher separately than that obtained using conventional two-step method (solvent system: methanol and chloroform. This one-step in situ method provides a fast and simple method to measure fatty acid methyl ester (FAME yields and could serve as a promising method to generate eicosapentaenoic acid methyl ester from microalgae.

Analyses of one-step liquid hydrogen production from methane and landfill gas

Energy Technology Data Exchange (ETDEWEB)

Huang, Cunping; T-Raissi, Ali [University of Central Florida, Florida Solar Energy Center, 1679 Clearlake Road, Cocoa, FL 32922-5703 (United States)

2007-11-15

Conventional liquid hydrogen (LH{sub 2}) production consists of two basic steps: (1) gaseous hydrogen (GH{sub 2}) production via steam methane reformation followed by purification by means of pressure swing adsorption (PSA), and (2) GH{sub 2} liquefaction. LH{sub 2} produced by the conventional processes is not carbon neutral because of the carbon dioxide (CO{sub 2}) emission from PSA operation. A novel concept is herein presented and flowsheeted for LH{sub 2} production with zero carbon emission using methane (CH{sub 4}) or landfill gas as feedstock. A cryogenic process is used for both H{sub 2} separation/purification and liquefaction. This one-step process can substantially increase the efficiency and reduce costs because no PSA step is required. Furthermore, the integrated process results in no CO{sub 2} emissions and minimal H{sub 2} losses. Of the five flowsheets presented, one that combines low and high temperature CO/CH{sub 4} reforming reactions in a single reactor shows the highest overall efficiency with the first and second law efficiencies of 85% and 56%, respectively. The latter figure assumes 10% overall energy loss and 30% efficiency for the cryogenic process. (author)

One-step synthesis of chlorinated graphene by plasma enhanced chemical vapor deposition

Energy Technology Data Exchange (ETDEWEB)

Fan, Liwei; Zhang, Hui; Zhang, Pingping; Sun, Xuhui, E-mail: xhsun@suda.edu.cn

2015-08-30

Highlights: • We developed a simple approach to synthesize the single layer chlorinated graphene. • CuCl{sub 2} on Cu surface is used as Cl source under the plasma treatment. • The formation of covalent C−Cl bond has been investigated by Raman and XPS. • Raman results indicate the p-type doping effect of chlorination. - Abstract: We developed an approach to synthesize the chlorinated single layer graphene (Cl-G) by one-step plasma enhanced chemical vapor deposition. Copper foil was simply treated with hydrochloric acid and then CuCl{sub 2} formed on the surface was used as Cl source under the assistance of plasma treatment. Compared with other two-step methods by post plasma/photochemical treatment of CVD-grown single layer graphene (SLG), one-step Cl-G synthesis approach is quite straightforward and effective. X-ray photoelectron spectroscopy (XPS) revealed that ∼2.45 atom% Cl remained in SLG. Compared with the pristine SLG, the obvious blue shifts of G band and 2D band along with the appearance of D’ band and D + G band in the Raman spectra indicate p-type doping of Cl-G.

Smear layer-deproteinizing improves bonding of one-step self-etch adhesives to dentin.

Science.gov (United States)

Thanatvarakorn, Ornnicha; Prasansuttiporn, Taweesak; Thittaweerat, Suppason; Foxton, Richard M; Ichinose, Shizuko; Tagami, Junji; Hosaka, Keiichi; Nakajima, Masatoshi

2018-03-01

Smear layer deproteinizing was proved to reduce the organic phase of smear layer covered on dentin surface. It was shown to eliminate hybridized smear layer and nanoleakage expression in resin-dentin bonding interface of two-step self-etch adhesive. This study aimed to investigate those effects on various one-step self-etch adhesives. Four different one-step self-etch adhesives were used in this study; SE One (SE), Scotchbond™ Universal (SU), BeautiBond Multi (BB), and Bond Force (BF). Flat human dentin surfaces with standardized smear layer were prepared. Smear layer deproteinizing was carried out by the application of 50ppm hypochlorous acid (HOCl) on dentin surface for 15s followed by Accel ® (p-toluenesulfinic acid salt) for 5s prior to adhesive application. No surface pretreatment was used as control. Microtensile bond strength (μTBS) and nanoleakage under TEM observation were investigated. The data were analyzed by two-way ANOVA and Tukey's post-hoc test and t-test at the significant level of 0.05. Smear layer deproteinizing significantly improved μTBS of SE, SU, and BB (player observed in control groups of SE, BB, and BF, and reticular nanoleakage presented throughout the hybridized complex in control groups of BB and BF were eliminated upon the smear layer deproteinizing. Smear layer deproteinizing by HOCl and Accel ® application could enhance the quality of dentin for bonding to one-step self-etch adhesives, resulting in the improving μTBS, eliminating hybridized smear layer and preventing reticular nanoleakage formation in resin-dentin bonding interface. Copyright © 2018 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

Fabrication of an all-polymer electrochemical sensor by using a one-step hot embossing procedure

DEFF Research Database (Denmark)

Kafka, Jan Robert; Larsen, Niels Bent; Skaarup, Steen

2010-01-01

We present a fast one-step hot embossing procedure for fabricating an all-polymer electrochemical sen¬sor based on a thin, conductive film of poly(3,4-ethylenedioxythiophene) (PEDOT), a few 100s of nano¬meters in thickness, polymerised on top of a non-conductive TOPAS® (Cyclic Olefin Copolymer) car...... of electrodes was achieved by spatial separation (Fig. 1). Functionality of the electrochemical system was shown by amperometric detection of physiologically relevant glucose concentrations (0–10 mM)....

Effectiveness of "Step into Health" program in Qatar: a pedometer-based longitudinal study.

Science.gov (United States)

Al-Kuwari, Mohamed G; Al-Mohannadi, Abdulla S; Sayegh, Suzan

2017-11-01

This study examines the impact of a one-year pedometer-based intervention on increasing the physical activity level among adult population in Qatar. This longitudinal study was conducted over a one-year period and included a total of 268 adults aged between 18-64 years old. Data were extracted and used from the "Step into Health" (SIH) program, a community-based program launched in 2012, as an approach to improve physical activity in Qatar. Walking intervention encouraged members of SIH to accumulate 10,000 steps or more per day and monitor their progress through a pedometer supported by a self-monitoring online account and a reinforcement system. This study shows a significant increase in average daily steps from 3933±3240 steps/day at baseline into 7507±5416 steps/week at the 12th month (P<0.001). It was found that 18.6% of participants met the daily target of 10,000 steps or more; however, there was a considerable increase of 39.2% by the 12th month. Females showed an increase in their physical activity; still, they remain less active than males. It was found that non-Arabs subgroup were more active than Arabs. Interestingly, older members (≥50 years old) were more active throughout the study period. Pedometer program was found to be effective in increasing the level of physical activity among participants. A decline in physical activity has been observed during hot weather, while re-enforcement campaign had a positive impact on the number of steps/day.

One-step fabrication of PEGylated fluorescent nanodiamonds through the thiol-ene click reaction and their potential for biological imaging

Science.gov (United States)

Huang, Hongye; Liu, Meiying; Tuo, Xun; Chen, Junyu; Mao, Liucheng; Wen, Yuanqing; Tian, Jianwen; Zhou, Naigen; Zhang, Xiaoyong; Wei, Yen

2018-05-01

Over the past years, fluorescent carbon nanoparticles have got growing interest for biological imaging. Fluorescent nanodiamonds (FNDs) are novel fluorescent carbon nanoparticles with multitudinous useful properties, including remarkable fluorescence properties, extremely low toxicity and high refractive index. However, facile preparation of FNDs with designable properties and functions from non-fluorescent detonation nanodiamonds (DNDs) has demonstrated to be challengeable. In this work, we reported for the first time that preparation of Polyethylene glycol (PEG) functionalized FNDs through a one-step thiol-ene click reaction using thiol containing PEG (PEG-SH) as the coating agent. Based on the characterization results, we demonstrated that PEG-SH could be efficiently introduced on DNDs to obtain FNDs through the thiol-ene click chemistry. The resultant FND-PEG composites showed high water dispersibility, strong fluorescence and low cytotoxicity. Moreover, FND-PEG composites could be internalized by cells and displayed good cell dyeing performance. All of these features implied that FND-PEG composites are of great potential for biological imaging. Taken together, a facile one-step strategy based on the one-step thiol-ene click reaction has been developed for efficient preparation of FND-PEG composites from non-fluorescent DNDs. The strategy should be also useful for fabrication of many other functional FNDs via using different thiol containing compounds for the universality of thiol-ene click reaction.

Customers’ Intention to Use Green Products: the Impact of Green Brand Dimensions and Green Perceived Value

Directory of Open Access Journals (Sweden)

Doszhanov Aibek

2015-01-01

Full Text Available This study aimed to identify the relationships between green brand dimension (green brand awareness, green brand image, and green brand trust, green perceived value and customer’s intention to use green products. Data was collected through structured survey questionnaire from 384 customers of three hypermarkets in Kuala-Lumpur. Data was analyzed based on multiple regression analysis. The results indicate that there are significant relationships between green brand awareness, green brand trust, green perceived value, and customer’s intention to use green products. However, green brand image was not found to have significant relationship with customer’s intention to use green products. The discussion presented suggestions for marketers and researchers interested in green branding.

Rapid detection of Prunus necrotic ringspot virus using magnetic nanoparticle-assisted reverse transcription loop-mediated isothermal amplification.

Science.gov (United States)

Zong, Xiaojuan; Wang, Wenwen; Wei, Hairong; Wang, Jiawei; Chen, Xin; Xu, Li; Zhu, Dongzi; Tan, Yue; Liu, Qingzhong

2014-11-01

Prunus necrotic ringspot virus (PNRSV) has seriously reduced the yield of Prunus species worldwide. In this study, a highly efficient and specific two-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) was developed to detect PNRSV. Total RNA was extracted from sweet cherry leaf samples using a commercial kit based on a magnetic nanoparticle technique. Transcripts were used as the templates for the assay. The results of this assay can be detected using agarose gel electrophoresis or by assessing in-tube fluorescence after adding SYBR Green I. The assay is highly specific for PNRSV, and it is more sensitive than reverse-transcription polymerase chain reaction (RT-PCR). Restriction enzyme digestion verified further the reliability of this RT-LAMP assay. To our knowledge, this is the first report of the application of RT-LAMP to PNRSV detection in Prunus species. Copyright © 2014 Elsevier B.V. All rights reserved.

One-step electrodeposition process of CuInSe2: Deposition time effect

Indian Academy of Sciences (India)

Administrator

CuInSe2 thin films were prepared by one-step electrodeposition process using a simplified two- electrodes system. ... homojunctions or heterojunctions (Rincon et al 1983). Efficiency of ... deposition times onto indium thin oxide (ITO)-covered.

The impact of green supply chain management in small to medium enterprises: Cross-sectional evidence

Directory of Open Access Journals (Sweden)

Chengedzai Mafini

2017-02-01

Full Text Available Background: South Africa has a high rate of small to medium enterprises (SMEs failure, especially in the manufacturing sector. The operational challenges confronting manufacturing SMEs are acknowledged by the Global Competitiveness Index that ranked South African SMEs as one of the lowest in emerging economies. Objectives: The aim of this study is to examine the association between green supply chain management (GSCM practices, environmental collaboration and financial performance in SMEs. Method: The study is quantitative in nature and involves a convenient sample of 312 SMEs based in Gauteng Province, South Africa. Data analyses follow a two-step process involving a confirmatory factor analysis to test the psychometric properties of the measurement scale and Structural Equation Modelling to test the proposed hypotheses. Results: The study shows that three GSCM practices, namely, green procurement, green logistics and green manufacturing in SMEs exert a positive effect on environmental collaboration, with green manufacturing exerting a higher effect than the other two constructs. In turn, higher levels of environmental collaboration inspired higher levels of SME financial performance. Conclusion: The study advances that SMEs can succeed financially through the influence of enhanced environmental collaboration, which emanates, in part, from the adoption and implementation of GSCM practices.

“One Step and Two Steps” freezing methods of dog’s semen in coconut water and ethylene glycol extender Métodos de congelamento "One Step" e "Two Steps" do sêmen de cães, diluído em solução de água de coco e etilenoglicol

Directory of Open Access Journals (Sweden)

Marco Antonio da Rocha Ferreira

2009-06-01

Full Text Available The objective of this study was to verify the influence of One Step and Two Steps freezing methods on dog’s spermatic viability. The experiment was performed using 20 ejaculates from 11 previously selected dogs which presented progressive motility over 70% and vigor equal or superior to 3. The samples were frozen in medium composed by coconut water and ethylene glycol as crioprotectant by the methods, One and Two Steps. After thawing, progressive motility, vigor, live spermatozoa percentage and plasmatic membrane integrity were evaluated. The results showed that there was no difference (P>0.05 in evaluated seminal characteristics. For progressive motility, the results (mean ± standard deviation were respectively for One and Two Steps, 47.3% ± 16.6% and 50.5% ±1 7.4%. As well as, the results for vigor, live spermatozoa percentage, plasmatic membrane integrity were 2.64 ± 0.67 and 2.9 ± 0.74; 52.2% ± 24.16 and 52.2% ± 24.48%; 15.27% ± 10.19% and 23.3% ± 11.6% for One and Two Steps methods, respectively. In conclusion, the One Step and Two Steps techniques can be used as semen cryopreservation methods in dog’s assisted reproduction and there is no difference between the evaluated characteristics of frozen-thawed semen using one or other technique.Objetivou-se, com este estudo, verificar a influência dos métodos de congelamento, One Step e Two Steps, na viabilidade espermática em cães. Foram utilizados 20 ejaculados de 11 cães, previamente selecionados, que apresentaram motilidade progressiva acima de 70% e vigor igual ou superior a três. As amostras foram congeladas em meio composto de água de coco e etilenoglicol, pelos métodos de One e Two Steps, e avaliadas após descongelamento, quanto à motilidade progressiva, vigor, porcentagem de espermatozóides vivos e integridade de membrana plasmática. Após o processo de diluição e congelamento One Step e Two Steps, verificou-se que não houve diferença (P>0,05 nas caracter

One-step reconstruction of the right inferior hepatic veins using auto-venous grafts in living-donor liver transplantation.

Science.gov (United States)

Ikegami, Toru; Shirabe, Ken; Yoshiya, Shohei; Soejima, Yuji; Yoshizumi, Tomoharu; Uchiyama, Hideaki; Toshima, Takeo; Motomura, Takashi; Maehara, Yoshihiko

2013-07-01

Reconstruction of the right inferior hepatic vein (RIHV) presents a major technical challenge in living donor liver transplantation (LDLT) using right lobe grafts. We studied 47 right lobe LDLT grafts with RIHV revascularization, comparing one-step reconstruction, performed post-May 2007 (n = 16), with direct anastomosis, performed pre-May 2007 (n = 31). In the one-step reconstruction technique, the internal jugular vein (n = 6), explanted portal vein (n = 5), inferior vena cava (n = 3), and shunt vessels (n = 2) were used as venous patch grafts for unifying the right hepatic vein, RIHVs, and middle hepatic vein tributaries. By 6 months after LDLT, there was no case of occlusion of the reconstructed RIHVs in the one-step reconstruction group, but a cumulative occlusion rate of 18.2 % in the direct anastomosis group. One-step reconstruction required a longer cold ischemic time (182 ± 40 vs. 115 ± 63, p one-step reconstruction and 83.9 % with direct anastomosis, respectively. One-step reconstruction of the RIHVs using auto-venous grafts is an easy and feasible technique promoting successful right lobe LDLT.

The impact of green logistics-based activities on the sustainable monetary expansion indicators of Oman

International Nuclear Information System (INIS)

Alshubiri, Faris

2017-01-01

The purpose of this study is to examine green logistic activities by three axes (financial economic, social and environment activities) and how these activities affect on sustainable monetary expansion indicators as an evidence of Sultanate of Oman. Design/methodology/approach: This study began by definition elements of logistic green and how evolution of this concept in recent years. This concept analyzed after survey of previous studies on green logistic. The independent variables of green logistic are includes of three components of financial economic , social and environment and applies these components to clarify the impact on expansionary monetary policy indicators ( broad , narrow and reserve money ) as a important signals in determining a country's economy. This study used data published in statistical annual report of central bank of Oman as representative of country economic of sultanate of Oman from the period 2008 to 2015. Findings: The results found two variables of government support to electricity sector (GSE) and subsidy on soft loans to private sector and housing (SSLPH) based on environment activities are statistical significant 1% and 5%. Only one variable of transport and communication (TC) in financial economic activates is statistical significant at 1% and 5% , but all variables community, social and personal (CSP) , cultural and religious affairs (CRA) and social security and welfare (SSW) in social activities are statistical significant at 1% 5% and 10% , finally , also the multiple regression test run of all variables of green logistics activities and each monetary expansion indicators and found there are a statistical significant at 1% and 5%, .The study recommends that should be attention with financial economic activities as a quantitative standard contributes to build the green logistic by diagnosed the priorities and existing economic and financial system that contributes of sustainable development system in the country

The impact of green logistics-based activities on the sustainable monetary expansion indicators of Oman

Energy Technology Data Exchange (ETDEWEB)

Alshubiri, Faris

2017-07-01

The purpose of this study is to examine green logistic activities by three axes (financial economic, social and environment activities) and how these activities affect on sustainable monetary expansion indicators as an evidence of Sultanate of Oman. Design/methodology/approach: This study began by definition elements of logistic green and how evolution of this concept in recent years. This concept analyzed after survey of previous studies on green logistic. The independent variables of green logistic are includes of three components of financial economic , social and environment and applies these components to clarify the impact on expansionary monetary policy indicators ( broad , narrow and reserve money ) as a important signals in determining a country's economy. This study used data published in statistical annual report of central bank of Oman as representative of country economic of sultanate of Oman from the period 2008 to 2015. Findings: The results found two variables of government support to electricity sector (GSE) and subsidy on soft loans to private sector and housing (SSLPH) based on environment activities are statistical significant 1% and 5%. Only one variable of transport and communication (TC) in financial economic activates is statistical significant at 1% and 5% , but all variables community, social and personal (CSP) , cultural and religious affairs (CRA) and social security and welfare (SSW) in social activities are statistical significant at 1% 5% and 10% , finally , also the multiple regression test run of all variables of green logistics activities and each monetary expansion indicators and found there are a statistical significant at 1% and 5%, .The study recommends that should be attention with financial economic activities as a quantitative standard contributes to build the green logistic by diagnosed the priorities and existing economic and financial system that contributes of sustainable development system in the country

The Influence of Legitimacy on a Proactive Green Orientation and Green Performance: A Study Based on Transitional Economy Scenarios in China

Directory of Open Access Journals (Sweden)

Baoshan Ge

2016-12-01

Full Text Available With environmental pollution, climate change and resource scarcity being serious global issues, green entrepreneurship is increasingly seen as an approach to simultaneously address economic performance, environmental impact and social responsibility. As green entrepreneurship needs to consider both venture performance and social responsibility, it will be subject to legitimacy constraints at the system level. Whether these legitimacy constraints are favorable to green enterprise is not yet clear from current research. Especially for transition economies, the problem of whether proactive green enterprises facing legitimacy constraints under institutional uncertainty can achieve green performance requires further study. Thus, a theoretical model to determine the relationship between green proactiveness orientation (GPO, green performance, legitimacy, and transitional economics was proposed. Based on the data from 235 new Chinese green firms, the empirical results suggest that green startups launch with a green proactiveness orientation, which enables them to acquire a green performance advantage over their competitors. Improvements in green performance is also shown to be driven by the pressure from institutional legitimacy. Better green performance can be easily achieved if green startups have a higher level of legitimacy. However, against the background of transitional economies, the increase in institutional uncertainty will damage the promotion of political legitimacy and make the enterprises that are subject to political legitimacy constraints lose their green performance. Currently, political legitimacy is no longer an impetus. However, the increase in institutional uncertainty will strengthen the promotion of commercial legitimacy and cause green-oriented startups to pursue more commercial interests. Thus, to a certain extent, it will lead to market uncertainty. The conclusion of this study not only provides guidance for startups in

Three-year randomized controlled clinical study of a one step universal adhesive and a two-step self-etch adhesive in Class II resin composite restorations

DEFF Research Database (Denmark)

van Dijken, Jan WV; Pallesen, Ulla

2017-01-01

Purpose: To evaluate in a randomized clinical evaluation the 3-year clinical durability of a one-step universal adhesive bonding system and compare it intraindividually with a 2-step self-etch adhesive in Class II restorations. Materials and Methods: Each of 57 participants (mean age 58.3 yr......) received at least two, as similar as possible, extended Class II restorations. The cavities in each of the 60 individual pairs of cavities were randomly distributed to the 1-step universal adhesive (All Bond Universal: AU) and the control 2-step self-etch adhesive (Optibond XTR: OX). A low shrinkage resin......) success rates (p>0.05). Annual failure rates were 1.8% and 2.6%, respectively.The main reason for failure was resin composite fracture. Conclusion: Class II resin composite restorations placed with a one-step universal adhesive showed good short time effectiveness....

Introducing of Green Pricing in the Korean Electricity Sector

Energy Technology Data Exchange (ETDEWEB)

Boo, K.J. [Korea Energy Economics Institute, Euiwang (Korea)

2001-11-01

renewables. Fourth, willingness to pay the premium is 17% with market potential of 14.2%. Fifth, the average rate of supporting the new renewable energy policies is less than 50%, way below those of the developed countries. These findings imply that consumer attitude toward green pricing is not so encouraging. At this point, what attracts our attention is the fact that the market potential of green pricing amounts to 14.2%, indicating the high level of environmental awareness. Materialization of this market potential calls for a step by step marketing strategy based on market segmentation. To start with, non-profit corporations should be targeted. Then, market efforts should be shifted to profit corporations. In addition, the government's role of leadership and monitoring is needed to help market function smoothly in a competitive environment. The major considerations in designing a green pricing are: first of all, the contribution-based green pricing is more desirable than the product-based or the capacity-based, in consideration of the current status of the domestic market development. Incentives for customer's wider participation include: protection against rate increases, related product discounts, certification and special recognition. Another idea to encourage participation is to enhance program's tangibility through locating the facilities in city parks and other public places frequently visited by community residents. What counts most in designing a green pricing is program credibility. To prevent a green scam, green pricing program should be accompanied by an education, green board of advisors, and environmental disclosure and green certification, all of which will make green pricing project transparent and accountable. This implies that existing programs and policies can be rekindled to supplement the green pricing program. In the midst of restructuring and transition into a competitive market, experts show a mixed feeling of concerns and hopes about

ALPPS and simultaneous right hemicolectomy - step one and resection of the primary colon cancer.

Science.gov (United States)

Fard-Aghaie, Mohammad H; Stavrou, Gregor A; Schuetze, Kim C; Papalampros, Alexandros; Donati, Marcello; Oldhafer, Karl J

2015-03-27

Resection of the liver is often limited due to the volume of the parenchyma. To address this problem, several approaches to induce hypertrophy were developed. Recently, the 'associating liver partition and portal vein ligation for staged hepatectomy' (ALPPS) procedure was introduced and led to rapid hypertrophy in a short interval. Additionally to the portal vein occlusion, the parenchyma is transected, which disrupts the inter-parenchymal vascular connections. Since the first description of the ALPPS procedure, various reports around the world were published. In some cases, due to the high morbidity and mortality, a decent oncologic algorithm is not deliverable in a timely manner. If a patient is to be treated with a liver-first approach, the resection of the primary could sometimes be severely protracted. To overcome the problem, a simultaneous resection of the primary tumor and step one of ALPPS were performed. A 73-year-old male patient underwent portal vein embolization (PVE) after suffering from a synchronous hepatic metastasized carcinoma of the right colic flexure in order to perform a right trisectionectomy. Sufficient hypertrophy could not be obtained by PVE. Thus a 'Rescue-ALPPS' was undertaken. During step one of ALPPS, we simultaneously performed a right hemicolectomy. The postoperative course after the first step was uneventful, and sufficient hypertrophy was achieved. In order to achieve a macroscopic disease-free state and lead the patient as soon as possible to the oncologic path (with, for example, chemotherapy), sometimes a simultaneous resection of the primary with step one of the ALPPS procedure seems justified. A resection of the primary with step two is not advisable, due to the high morbidity and mortality after this step. This case shows that a simultaneous resection is feasible and safe. Whether other locations of the primary should be treated this way must be part of further investigations.

DNA microsatellite region for a reliable quantification of soft wheat adulteration in durum wheat-based foodstuffs by real-time PCR.

Science.gov (United States)

Sonnante, Gabriella; Montemurro, Cinzia; Morgese, Anita; Sabetta, Wilma; Blanco, Antonio; Pasqualone, Antonella

2009-11-11

Italian industrial pasta and durum wheat typical breads must be prepared using exclusively durum wheat semolina. Previously, a microsatellite sequence specific of the wheat D-genome had been chosen for traceability of soft wheat in semolina and bread samples, using qualitative and quantitative Sybr green-based real-time experiments. In this work, we describe an improved method based on the same soft wheat genomic region by means of a quantitative real-time PCR using a dual-labeled probe. Standard curves based on dilutions of 100% soft wheat flour, pasta, or bread were constructed. Durum wheat semolina, pasta, and bread samples were prepared with increasing amounts of soft wheat to verify the accuracy of the method. Results show that reliable quantifications were obtained especially for the samples containing a lower amount of soft wheat DNA, fulfilling the need to verify labeling of pasta and typical durum wheat breads.

The One-Step Pickering Emulsion Polymerization Route for Synthesizing Organic-Inorganic Nanocomposite Particles

Directory of Open Access Journals (Sweden)

Kaushal Rege

2010-02-01

Full Text Available Polystyrene-silica core-shell nanocomposite particles are successfully prepared via one-step Pickering emulsion polymerization. Possible mechanisms of Pickering emulsion polymerization are addressed in the synthesis of polystyrene-silica nanocomposite particles using 2,2-azobis(2-methyl-N-(2-hydroxyethylpropionamide (VA-086 and potassium persulfate (KPS as the initiator. Motivated by potential applications of “smart” composite particles in controlled drug delivery, the one-step Pickering emulsion polymerization route is further applied to synthesize polystyrene/poly(N-isopropylacrylamide (PNIPAAm-silica core-shell nanoparticles with N-isopropylacrylamide incorporated into the core as a co-monomer. The polystyrene/PNIPAAm-silica composite nanoparticles are temperature sensitive and can be taken up by human prostate cancer (PC3-PSMA cells.

Fast nanostructured carbon microparticle synthesis by one-step high-flux plasma processing

NARCIS (Netherlands)

Aussems, D. U. B.; Bystrov, K.; Dogan, I.; Arnas, C.; Cabié, M.; Neisius, T.; Rasinski, M.; Zoethout, E.; Lipman, P.; van de Sanden, M. C. M.; Morgan, T. W.

2017-01-01

This study demonstrates a fast one-step synthesis method for nanostructured carbon microparticles on graphite samples using high-flux plasma exposure. These structures are considered as potential candidates for energy applications such as Li-ion batteries and supercapacitors. The samples were

Fast nanostructured carbon microparticle synthesis by one-step high-flux plasma processing

NARCIS (Netherlands)

Aussems, D.U.B.; Bystrov, K.E.; Doǧan, I.; Arnas, C.; Cabié, M.; Neisius, T.; Rasinski, M.; Lipman, P.J.L.; van de Sanden, M.C.M.; Morgan, T.W.

This study demonstrates a fast one-step synthesis method for nanostructured carbon microparticles on graphite samples using high-flux plasma exposure. These structures are considered as potential candidates for energy applications such as Li-ion batteries and supercapacitors. The samples were

One-Step Real-Image Reflection Holograms

Science.gov (United States)

Buah-Bassuah, Paul K.; Vannoni, Maurizio; Molesini, Giuseppe

2007-01-01

A holographic process is presented where the object is made of the real image produced by a two-mirror system. Single-step reflection hologram recording is achieved. Details of the process are given, optics concepts are outlined and demonstrative results are presented. (Contains 6 figures and 2 footnotes.)

Centrifuge modeling of one-step outflow tests for unsaturated parameter estimations

Directory of Open Access Journals (Sweden)

H. Nakajima

2006-01-01

Full Text Available Centrifuge modeling of one-step outflow tests were carried out using a 2-m radius geotechnical centrifuge, and the cumulative outflow and transient pore water pressure were measured during the tests at multiple gravity levels. Based on the scaling laws of centrifuge modeling, the measurements generally showed reasonable agreement with prototype data calculated from forward simulations with input parameters determined from standard laboratory tests. The parameter optimizations were examined for three different combinations of input data sets using the test measurements. Within the gravity level examined in this study up to 40g, the optimized unsaturated parameters compared well when accurate pore water pressure measurements were included along with cumulative outflow as input data. With its capability to implement variety of instrumentations under well controlled initial and boundary conditions and to shorten testing time, the centrifuge modeling technique is attractive as an alternative experimental method that provides more freedom to set inverse problem conditions for the parameter estimation.

Centrifuge modeling of one-step outflow tests for unsaturated parameter estimations

Science.gov (United States)

Nakajima, H.; Stadler, A. T.

2006-10-01

Centrifuge modeling of one-step outflow tests were carried out using a 2-m radius geotechnical centrifuge, and the cumulative outflow and transient pore water pressure were measured during the tests at multiple gravity levels. Based on the scaling laws of centrifuge modeling, the measurements generally showed reasonable agreement with prototype data calculated from forward simulations with input parameters determined from standard laboratory tests. The parameter optimizations were examined for three different combinations of input data sets using the test measurements. Within the gravity level examined in this study up to 40g, the optimized unsaturated parameters compared well when accurate pore water pressure measurements were included along with cumulative outflow as input data. With its capability to implement variety of instrumentations under well controlled initial and boundary conditions and to shorten testing time, the centrifuge modeling technique is attractive as an alternative experimental method that provides more freedom to set inverse problem conditions for the parameter estimation.

Highly efficient one-pot/one-step synthesis of multiblock copolymers from three-component polymerization of carbon dioxide, epoxide and lactone.

Science.gov (United States)

Li, Yang; Hong, Jiali; Wei, Renjian; Zhang, Yingying; Tong, Zaizai; Zhang, Xinghong; Du, Binyang; Xu, Junting; Fan, Zhiqiang

2015-02-01

It is a long-standing challenge to combine mixed monomers into multiblock copolymer (MBC) in a one-pot/one-step polymerization manner. We report the first example of MBC with biodegradable polycarbonate and polyester blocks that were synthesized from highly efficient one-pot/one-step polymerization of cyclohexene oxide (CHO), CO 2 and ε-caprolactone (ε-CL) in the presence of zinc-cobalt double metal cyanide complex and stannous octoate. In this protocol, two cross-chain exchange reactions (CCER) occurred at dual catalysts respectively and connected two independent chain propagation procedures ( i.e. , polycarbonate formation and polyester formation) simultaneously in a block-by-block manner, affording MBC without tapering structure. The multiblock structure of MBC was determined by the rate ratio of CCER to the two chain propagations and could be simply tuned by various kinetic factors. This protocol is also of significance due to partial utilization of renewable CO 2 and improved mechanical properties of the resultant MBC.

Development of Singlet Oxygen Luminescence Kinetics during the Photodynamic Inactivation of Green Algae

Directory of Open Access Journals (Sweden)

Tobias Bornhütter

2016-04-01

Full Text Available Recent studies show the feasibility of photodynamic inactivation of green algae as a vital step towards an effective photodynamic suppression of biofilms by using functionalized surfaces. The investigation of the intrinsic mechanisms of photodynamic inactivation in green algae represents the next step in order to determine optimization parameters. The observation of singlet oxygen luminescence kinetics proved to be a very effective approach towards understanding mechanisms on a cellular level. In this study, the first two-dimensional measurement of singlet oxygen kinetics in phototrophic microorganisms on surfaces during photodynamic inactivation is presented. We established a system of reproducible algae samples on surfaces, incubated with two different cationic, antimicrobial potent photosensitizers. Fluorescence microscopy images indicate that one photosensitizer localizes inside the green algae while the other accumulates along the outer algae cell wall. A newly developed setup allows for the measurement of singlet oxygen luminescence on the green algae sample surfaces over several days. The kinetics of the singlet oxygen luminescence of both photosensitizers show different developments and a distinct change over time, corresponding with the differences in their localization as well as their photosensitization potential. While the complexity of the signal reveals a challenge for the future, this study incontrovertibly marks a crucial, inevitable step in the investigation of photodynamic inactivation of biofilms: it shows the feasibility of using the singlet oxygen luminescence kinetics to investigate photodynamic effects on surfaces and thus opens a field for numerous investigations.

Green and Bio-Based Solvents.

Science.gov (United States)

Calvo-Flores, Francisco G; Monteagudo-Arrebola, María José; Dobado, José A; Isac-García, Joaquín

2018-04-24

Chemical reactions and many of the procedures of separation and purification employed in industry, research or chemistry teaching utilize solvents massively. In the last decades, with the birth of Green Chemistry, concerns about the employment of solvents and the effects on human health, as well as its environmental impacts and its dependence on non-renewable raw materials for manufacturing most of them, has drawn the attention of the scientific community. In this work, we review the concept of green solvent and the properties and characteristics to be considered green. Additionally, we discuss the different possible routes to prepare many solvents from biomass, as an alternative way to those methods currently applied in the petrochemical industry.

Green roof Malta

OpenAIRE

Gatt, Antoine

2015-01-01

In Malta, buildings cover one third of the Island, leaving greenery in the dirt track. Green roofs are one way to bring plants back to urban areas with loads of benefits. Antoine Gatt, who manages the LifeMedGreenRoof project at the University of Malta, tells us more. http://www.um.edu.mt/think/green-roof-malta/

A green chemoselective one-pot protocol for expeditious synthesis of symmetric pyranodipyrimidine derivatives using ZrOCl2.8H2O

Directory of Open Access Journals (Sweden)

Mehdi Rimaz

2016-09-01

Full Text Available A convenient, highly efficient and time economic method has been described for the chemo- and regioselective synthesis of 5-aryloyl-1,3,7,9-tetraalkyl-2,8-dithioxo-2,3,8,9-tetrahydro-1H-pyrano[2,3-d:6,5-dˊ]dipyrimidine-4,6(5H,7H-diones derivatives by one-pot two-component reaction of 1,3-diethyl-2-thiobarbituric acid or 1,3-dimethyl-2-thiobarbituric acid with substituted arylglyoxalmonohydrates using commercially available zirconium (IV oxydichloride octahydrate (ZrOCl2.8H2O as green Lewis acid catalyst. This method is associated with some attractive characteristics such as good selectivity, very short reaction time, high yield of products, cleaner reaction profile, no harmful by-product, cheap and environmental benign catalyst, simple experimental and work-up procedure. This procedure does not require solvent separation and purification steps such as column chromatography.

One-step prepared cobalt-based nanosheet as an efficient heterogeneous catalyst for activating peroxymonosulfate to degrade caffeine in water.

Science.gov (United States)

Lin, Kun-Yi Andrew; Lai, Hong-Kai; Tong, Shaoping

2018-03-15

Two-dimensional (2D) planar cobalt-containing materials are promising catalysts for activating peroxymonosulfate (PMS) to degrade contaminants because 2D sheet-like morphology provides large reactive surfaces. However, preparation of these sheet-supported cobaltic materials typically involves multiple steps and complex reagents, making them less practical for PMS activation. In this study, a cobalt-based nanosheet (CoNS) is particularly developed using a one-step hydrothermal process with a single reagent in water. The resulting CoNS can exhibit a thickness as thin as a few nanometers and 2-D morphology. CoNS is also primarily comprised of cobalt species in a coordinated form of Prussian Blue analogue, which consists of both Co 3+ and Co 2+ . These features make CoNS promising for activating PMS in aqueous systems. As degradation of an emerging contaminant, caffeine, is selected as a representative reaction, CoNS not only successfully activates PMS to fully degrade caffeine in 20 min but also exhibits a much higher catalytic activity than the most common PMS activator, Co 3 O 4 . Via studying inhibitive effects of radical scavengers, caffeine degradation by CoNS-activated PMS is primarily attributed to sulfate radicals and hydroxyl radicals to a lesser extent. The degradation products of caffeine by CoNS-activated PMS are also identified and a potential degradation pathway is proposed. Moreover, CoNS could be also re-used to activate PMS for caffeine degradation without activity loss. These results indicate that CoNS is a conveniently prepared and highly effective and stable 2-D catalyst for aqueous chemical oxidation reactions. Copyright © 2017. Published by Elsevier Inc.

[Optimization of urban green space spatial arrangement based on supply-demand analysis: a case study in Nanjing City, China].

Science.gov (United States)

Gui, Kun-Peng; Xu, Jian-Gang; Zhang, Xiang

2013-05-01

Urban green space has the functions of ecological and social services, and the two services levels are decided by the supply-demand relationship. However, the supply-demand of green space not only involves in quantity, but also refers to spatial distribution. Therefore, only greening indicators can not wholly reflect the true levels of green space services. Based on the supply-demand analysis and supported by the ArcGIS, this paper examined the ecological and social services levels of the urban green spaces in Nanjing City by using the evenness indicator and the rate the people could enjoy the public green space in their accessible area. Accordingly, the ecological and social services levels of the green space in the City were investigated. The results showed that in the east of Nanjing City, green spaces were rich, but high accessible ones were lack, which resulted in a moderate social service level. In the center of the City, green spaces were lack and distributed unevenly, resulting in the low levels of ecological and social services. In Hexi area, due to the shortage in ecological green space and its uneven distribution, the green spaces had a high level social service but a low level ecological service. In the southern and northern areas of the City, green spaces were in deficiency, uneven distribution, and lack in high accessible.

Development of the Multiple Gene Knockout System with One-Step PCR in Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius

Directory of Open Access Journals (Sweden)

Shoji Suzuki

2017-01-01

Full Text Available Multiple gene knockout systems developed in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius are powerful genetic tools. However, plasmid construction typically requires several steps. Alternatively, PCR tailing for high-throughput gene disruption was also developed in S. acidocaldarius, but repeated gene knockout based on PCR tailing has been limited due to lack of a genetic marker system. In this study, we demonstrated efficient homologous recombination frequency (2.8 × 104 ± 6.9 × 103 colonies/μg DNA by optimizing the transformation conditions. This optimized protocol allowed to develop reliable gene knockout via double crossover using short homologous arms and to establish the multiple gene knockout system with one-step PCR (MONSTER. In the MONSTER, a multiple gene knockout cassette was simply and rapidly constructed by one-step PCR without plasmid construction, and the PCR product can be immediately used for target gene deletion. As an example of the applications of this strategy, we successfully made a DNA photolyase- (phr- and arginine decarboxylase- (argD- deficient strain of S. acidocaldarius. In addition, an agmatine selection system consisting of an agmatine-auxotrophic strain and argD marker was also established. The MONSTER provides an alternative strategy that enables the very simple construction of multiple gene knockout cassettes for genetic studies in S. acidocaldarius.

Green chemicals : A Kinetic Study on the Conversion of Glucose to Levulinic Acid

NARCIS (Netherlands)

Girisuta, B.; Janssen, L.P.B.M.; Heeres, H.J.

2006-01-01

Levulinic acid has been identified as a promising green, biomass derived platform chemical. A kinetic study on one of the key steps in the conversion of biomass to levulinic acid, i.e., the acid catalysed decomposition of glucose to levulinic acid has been performed. The experiments were performed

Amplify-and-Forward Cooperative Diversity for Green UWB-Based WBSNs

Directory of Open Access Journals (Sweden)

Heba Shaban

2013-01-01

Full Text Available This paper proposes a novel green cooperative diversity technique based on suboptimal template-based ultra-wideband (UWB wireless body sensor networks (WBSNs using amplify-and-forward (AF relays. In addition, it analyzes the bit-error-rate (BER performance of the proposed nodes. The analysis is based on the moment-generating function (MGF of the total signal-to-noise ratio (SNR at the destination. It also provides an approximate value for the total SNR. The analysis studies the performance of equally correlated binary pulse position modulation (EC-BPPM assuming the sinusoidal and square suboptimal template pulses. Numerical results are provided for the performance evaluation of optimal and suboptimal template-based nodes with and without relay cooperation. Results show that one relay node provides ~23 dB performance enhancement at BER, which mitigates the effect of the nondesirable non-line-of-sight (NLOS links in WBSNs.

The optoelectronic chameleon - GaN-based light emitters from the UV to green

Energy Technology Data Exchange (ETDEWEB)

Kneissl, Michael [Institut fuer Festkoerperphysik, Technische Universitaet Berlin (Germany)

2008-07-01

Group III-nitrides have evolved into one of the most versatile and important semiconductor materials for optoelectronic devices. GaN-based blue, green and white light emitting diodes have already entered many parts of everyday life and violet lasers are expected to be following soon. However, considering the extraordinary electronic properties and the wide spectral range that is accessible through nitride materials, it appears that it we have just touched the tip of the iceberg. We discuss some of the new fields of research for InAlGaN materials and devices and review progress in the development of near and deep ultraviolet light emitting diodes, as well as growth and optical properties of InN and indium rich InGaN alloys for emitter in the blue-green spectral range and beyond.

A One-Step Route to CO2 -Based Block Copolymers by Simultaneous ROCOP of CO2 /Epoxides and RAFT Polymerization of Vinyl Monomers.

Science.gov (United States)

Wang, Yong; Zhao, Yajun; Ye, Yunsheng; Peng, Haiyan; Zhou, Xingping; Xie, Xiaolin; Wang, Xianhong; Wang, Fosong

2018-03-26

The one-step synthesis of well-defined CO 2 -based diblock copolymers was achieved by simultaneous ring-opening copolymerization (ROCOP) of CO 2 /epoxides and RAFT polymerization of vinyl monomers using a trithiocarbonate compound bearing a carboxylic group (TTC-COOH) as the bifunctional chain transfer agent (CTA). The double chain-transfer effect allows for independent and precise control over the molecular weight of the two blocks and ensures narrow polydispersities of the resultant block copolymers (1.09-1.14). Notably, an unusual axial group exchange reaction between the aluminum porphyrin catalyst and TTC-COOH impedes the formation of homopolycarbonates. By taking advantage of the RAFT technique, it is able to meet the stringent demand for functionality control to well expand the application scopes of CO 2 -based polycarbonates. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Green chemistry: A tool in Pharmaceutical Chemistry

OpenAIRE

Smita Talaviya; Falguni Majumdar

2012-01-01

Green chemistry expresses an area of research developing from scientific discoveries about pollution awareness and it utilizes a set of principles that reduces or eliminates the use or generation of hazardous substances in all steps of particular synthesis or process. Chemists and medicinal scientists can greatly reduce the risk to human health and the environment by following all the valuable principles of green chemistry. The most simple and direct way to apply green chemistry in pharmaceut...

The quantitative inspection of iron aluminide green sheet using transient thermography

International Nuclear Information System (INIS)

Watkins, Michael L.; Hinders, Mark K.; Scorey, Clive; Winfree, William

1999-01-01

The recent development of manufacturing techniques for the fabrication of thin iron aluminide, FeAl, sheet requires advanced quantitative methods for on-line inspection. An understanding of the mechanisms responsible for flaws and the development of appropriate flaw detection methods are key elements in an effective quality management system. The first step in the fabrication of thin FeAl alloy sheet is the formation of a green sheet, either by cold rolling or tape casting FeAl powder mixed with organic binding agents. The finished sheet is obtained using a series of process steps involving binder elimination, densification, sintering, and annealing. Non-uniformities within the green sheet are the major contributor to material failure in subsequent sheet processing and the production of non-conforming finished sheet. Previous work has demonstrated the advantages of using active thermography to detect the flaws and heterogeneity within green powder composites (1)(2)(3). The production environment and physical characteristics of these composites provide for unique challenges in developing a rapid nondestructive inspection capability. Thermography is non-contact and minimizes the potential damage to the fragile green sheet. Limited access to the material also demands a one-sided inspection technique. In this paper, we will describe the application of thermography for 100% on-line inspection within an industrial process. This approach is cost competitive with alternative technologies, such as x-ray imaging systems, and provides the required sensitivity to the variations in material composition. The formation of green sheet flaws and their transformation into defects within intermediate and finished sheet products will be described. A green sheet conformance criterion will be presented which would significantly reduce the probability of processing poor quality green sheet which contributes to higher waste and inferior bulk alloy sheet

One-step trinary signed-digit arithmetic using an efficient encoding scheme

Science.gov (United States)

Salim, W. Y.; Fyath, R. S.; Ali, S. A.; Alam, Mohammad S.

2000-11-01

The trinary signed-digit (TSD) number system is of interest for ultra fast optoelectronic computing systems since it permits parallel carry-free addition and borrow-free subtraction of two arbitrary length numbers in constant time. In this paper, a simple coding scheme is proposed to encode the decimal number directly into the TSD form. The coding scheme enables one to perform parallel one-step TSD arithmetic operation. The proposed coding scheme uses only a 5-combination coding table instead of the 625-combination table reported recently for recoded TSD arithmetic technique.

One-Step Generation of Multiqubit Greenberger-Horne-Zeilinger States in a Driven Circuit QED System

International Nuclear Information System (INIS)

Huang Jinsong; Nie Wei; Wei Lianfu

2011-01-01

We propose an efficient scheme to generate multiqubit Greenberger-Horne-Zeilinger (GHZ) states by one-step quantum operation in a driven circuit quantum electrodynamics (QED) system. Our proposal is based on a unitary evolution exp[-iλS 2 x ], with S x being the collective spin operator in x direction and λ a controllable parameter, induced by driving the resonator. The quantum operation avoids resonator-field decay and may achieve the GHZ states with ideal success probability. The feasibility with the experimentally-demonstrated circuit QED system is also discussed. (general)

Comprehensive and Rapid Real-Time PCR Analysis of 21 Foodborne Outbreaks

Directory of Open Access Journals (Sweden)

Hiroshi Fukushima

2009-01-01

Full Text Available A set of four duplex SYBR Green I PCR (SG-PCR assay combined with DNA extraction using QIAamp DNA Stool Mini kit was evaluated for the detection of foodborne bacteria from 21 foodborne outbreaks. The causative pathogens were detected in almost all cases in 2 hours or less. The first run was for the detection of 8 main foodborne pathogens in 5 stool specimens within 2 hours and the second run was for the detection of other unusual suspect pathogens within a further 45 minutes. After 2 to 4 days, the causative agents were isolated and identified. The results proved that for comprehensive and rapid molecular diagnosis in foodborne outbreaks, Duplex SG-PCR assay is not only very useful, but is also economically viable for one-step differentiation of causative pathogens in fecal specimens obtained from symptomatic patients. This then allows for effective diagnosis and management of foodborne outbreaks.

Silver nanowires as the current collector for a flexible in-plane micro-supercapacitor via a one-step, mask-free patterning strategy

Science.gov (United States)

Liu, Lang; Li, Han-Yu; Yu, Yao; Liu, Lin; Wu, Yue

2018-02-01

The fabrication of a current collector-contained in-plane micro-supercapacitor (MSC) usually requires the patterning of the current collector first and then subsequent patterning of the active material with the assistance of a photoresist and mask. However, this two-step patterning process is too complicated and the photoresist used is harmful to the properties of nanomaterials. Here, we demonstrate a one-step, mask-free strategy to pattern the current collector and the active material at the same time, for the fabrication of an all-solid-state flexible in-plane MSC. Silver nanowires (AgNWs) are used as the current collector. An atmospheric pressure pulsed cold micro-plasma-jet is used to realize the one-step, mask-free production of interdigitated multi-walled carbon nanotube (MWCNT)/AgNW electrodes. Remarkably, the fabricated MWCNT/AgNW-based MSC shows good flexibility and excellent rate capability. Moreover, the performance of properties including cyclic stability, equivalent series resistance, relaxation time and energy/power densities of the MWCNT/AgNW-based MSC are significantly enhanced by the presence of the AgNW current collector.

One step processing for future diesel specifications

International Nuclear Information System (INIS)

Brierley, G.R.

1997-01-01

The trend in diesel fuel specifications is to limit the sulfur level to less than 0.05 wt- per cent. Many regions have also specified that diesel fuels must have lower aromatic levels, higher cetane numbers, and lower distillation end points. These changes will require significant refinery investment to meet the new diesel fuel specifications. The changes may also significantly affect the value of synthetic crude stocks. UOP has developed a new hydroprocessing catalyst which makes it possible to meet the new diesel specifications in one single processing step and at minimal cost. The catalyst saturates aromatics while opening ring structures at the same time. By selectively cracking heavy components into the diesel range with minimal cracking to gas or naphtha, heavier feedstocks can be upgraded to diesel, and refinery diesel yield can be augmented. Synthetic crude distillate is often high in aromatics and low in cetane number. This new UOP hydroprocessing system will allow synthetic crude producers and refiners to produce diesel fuels with higher cetane numbers, high-quality distillate blendstocks and distillate fuels. 26 figs

An optimized fed-batch culture strategy integrated with a one-step fermentation improves L-lactic acid production by Rhizopus oryzae.

Science.gov (United States)

Fu, Yongqian; Sun, Xiaolong; Zhu, Huayue; Jiang, Ru; Luo, Xi; Yin, Longfei

2018-05-21

In previous work, we proposed a novel modified one-step fermentation fed-batch strategy to efficiently generate L-lactic acid (L-LA) using Rhizopus oryzae. In this study, to further enhance efficiency of L-LA production through one-step fermentation in fed-batch cultures, we systematically investigated the initial peptone- and glucose-feeding approaches, including different initial peptone and glucose concentrations and maintained residual glucose levels. Based on the results of this study, culturing R. oryzae with initial peptone and glucose concentrations of 3.0 and 50.0 g/l, respectively, using a fed-batch strategy is an effective approach of producing L-LA through one-step fermentation. Changing the residual glucose had no obvious effect on the generation of L-LA. We determined the maximum LA production and productivity to be 162 g/l and 6.23 g/(l·h), respectively, during the acid production stage. Compared to our previous work, there was almost no change in L-LA production or yield; however, the productivity of L-LA increased by 14.3%.

Facile one-step synthesis and photoluminescence properties of Ag–ZnO core–shell structure

International Nuclear Information System (INIS)

Zhai, HongJu; Wang, LiJing; Han, DongLai; Wang, Huan; Wang, Jian; Liu, XiaoYan; Lin, Xue; Li, XiuYan; Gao, Ming; Yang, JingHai

2014-01-01

Graphical abstract: The PL of the Ag–ZnO core-shell nanostructure showed obvious increase of UV emission and slight decrease of visible light emission compared to that of the pure ZnO. With the calcination temperature increasing from 300 to 600 °C, the primary peak located at 380 nm became stronger and sharper, indicating that the increasing calcination temperature made the samples crystallize better. - Highlights: • Ag-ZnO core-shell structure was obtained via a simple one-step solvothermal process. • The approach was simple, mild, low cost, reproducible and easy-to-handle. • The obvious enhancement of UV luminescent has been observed. • Effects of the calcining temperature to luminescence were investigated in detail. - Abstract: Ag–ZnO core–shell structures were gained via one-step solvothermal process. The products were characterized by means of X-ray diffraction (XRD), transmission electron microscopy (TEM), Raman spectroscopy, photoluminescence (PL) and UV–vis spectroscopy, respectively. It was shown that the properties were greatly changed compared to pure ZnO from the PL and Raman spectra, which indicated the strong interfacial interaction between ZnO and Ag. The work provides a feasible method to synthesize Ag–ZnO core–shell structure photocatalyst, which is promising in the further practical application of ZnO-based photocatalytic materials

Effect of one step KOH activation and CaO modified carbon in transesterification reaction

Science.gov (United States)

Yacob, Abd Rahim; Zaki, Muhammad Azam Muhammad

2017-11-01

In this work, one step activation was introduced using potassium hydroxide (KOH) and calcium oxide (CaO) modified palm kernel shells. Various concentration of calcium oxide was used as catalyst while maintaining the same concentration of potassium hydroxide to activate and impregnate the palm kernel shell before calcined at 500°C for 5 hours. All the prepared samples were characterized using Fourier Transform Infrared (FTIR) and Field Emission Scanning Electron Microscope (FESEM). FTIR analysis of raw palm kernel shell showed the presence of various functional groups. However, after activation, most of the functional groups were eliminated. The basic strength of the prepared samples were determined using back titration method. The samples were then used as base heterogeneous catalyst for the transesterification reaction of rice bran oil with methanol. Analysis of the products were performed using Gas Chromatography Flame Ionization Detector (GC-FID) to calculate the percentage conversion of the biodiesel products. This study shows, as the percentage of one step activation potassium and calcium oxide doped carbon increases thus, the basic strength also increases followed by the increase in biodiesel production. Optimization study shows that the optimum biodiesel production was at 8 wt% catalyst loading, 9:1 methanol: oil molar ratio at 65°C and 6 hours which gives a conversion up to 95%.

Extending green technology innovations to enable greener fabs

Science.gov (United States)

Takahisa, Kenji; Yoo, Young Sun; Fukuda, Hitomi; Minegishi, Yuji; Enami, Tatsuo

2015-03-01

Semiconductor manufacturing industry has growing concerns over future environmental impacts as fabs expand and new generations of equipment become more powerful. Especially rare gases supply and price are one of prime concerns for operation of high volume manufacturing (HVM) fabs. Over the past year it has come to our attention that Helium and Neon gas supplies could be unstable and become a threat to HVM fabs. To address these concerns, Gigaphoton has implemented various green technologies under its EcoPhoton program. One of the initiatives is GigaTwin deep ultraviolet (DUV) lithography laser design which enables highly efficient and stable operation. Under this design laser systems run with 50% less electric energy and gas consumption compared to conventional laser designs. In 2014 we have developed two technologies to further reduce electric energy and gas efficiency. The electric energy reduction technology is called eGRYCOS (enhanced Gigaphoton Recycled Chamber Operation System), and it reduces electric energy by 15% without compromising any of laser performances. eGRYCOS system has a sophisticated gas flow design so that we can reduce cross-flow-fan rotation speed. The gas reduction technology is called eTGM (enhanced Total gas Manager) and it improves gas management system optimizing the gas injection and exhaust amount based on laser performances, resulting in 50% gas savings. The next steps in our roadmap technologies are indicated and we call for potential partners to work with us based on OPEN INNOVATION concept to successfully develop faster and better solutions in all possible areas where green innovation may exist.

ONE STEP SYNTHESIS OF MAGNETIC PARTICLES COVERED WITH CASEIN SURFACTANT

OpenAIRE

Urquijo Morales, Jeaneth Patricia; Casanova Yepes, Herley; Morales Aramburo, Álvaro Luis; Zysler, Roberto Daniel

2014-01-01

The one-step coprecipitation method is used to obtain magnetic nanoparticles controlling the pH (10 and 12), and casein surfactant (CS) concentrations (1 % and 3 % (m/m)). CS has not been used so far for stabilizing magnetic iron oxide ferrofluids. The magnetic nanoparticles have a magnetite core with maghemite in surface, and a shell of polymer. The transmission electron images confirm the crystallinity, particle size distribution in the range of 5-10 nm, and the spinel structure of the nano...

One-step synthesis and microstructure of CuO-SDC composites

Energy Technology Data Exchange (ETDEWEB)

Firmino, H.C.T.; Araujo, A.J.M.; Dutra, R.P.S.; Macedo, D.A., E-mail: hellentorrano@hotmail.com, E-mail: allanjp1993@hotmail.com, E-mail: ricardopsd@gmail.com, E-mail: damaced@gmail.com [Universidade Federal da Paraiba (UFPB), Joao Pessoa, PB (Brazil); Nascimento, R.M., E-mail: rmaribondo@ufrnet.br [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil); Rajesh, S., E-mail: rajeshayr@gmail.com [University of Aveiro (Portugal)

2017-01-15

An in situ one step synthesis route based on the polymeric precursor method was used to produce dual phase CuO-samaria doped ceria (SDC) nanocomposite powders. This chemical route allowed to obtain composite powders with reduced particle size and uniform distribution of Cu, Ce and Sm elements. The particulate material was characterized by powder X-ray diffraction (XRD) combined with Rietveld refinement. CuO-SDC sintered in air between 950 to 1050 °C and subsequently reduced to Cu-SDC cermets were further characterized by XRD and scanning electron microscopy. The open porosity was measured using the Archimedes' principle. Suitable microstructures for both charge transfer and mass transport processes (30 to 45% porosity) were attained in Cu-SDC cermets previously fired at 1000 to 1050 °C. Overall results indicated that CuO-SDC composites and Cu-SDC cermets with potential application as anodes for solid oxide fuel cells (SOFCs) can be obtained by microstructural design. An anode supported half-cell was prepared by co-pressing and co-firing gadolinia doped ceria (CGO) and the herein synthesized CuO-SDC nanocomposite powder. (author)

Green Energy-Industrial Innovation: A Comparative Study of Green Energy Transformations in Northern Europe

Energy Technology Data Exchange (ETDEWEB)

Eikeland, Per Ove; Christiansen, Atle Christer; Koefoed, Anne Louise; Midttun, Atle; Tangen, Kristian

1999-07-01

Greening of industry is a prominent topic on the European agenda and has received a new impetus with the Kyoto process, where the European Union and its member states are facing new obligations. Due to its role as a major infrastructure sector and due to its extensive emissions, the electricity sector has become a focal industry in this context. These same factors, as well as the large public ownership has, in fact, traditionally placed energy in a central public political focus. The energy and electricity sector hence exemplify the intertwined nature of economics and politics in sector developments, where technology choices and capacity expansions have numerous motivations and diverse interests to please. This report discusses ''green'' innovation in the energy industry. By selecting some of the most advanced examples of breakthrough for new green technologies in Europe, it explores the basic elements of successful greening of industry. To account for the fact that product innovation- and diffusion-processes are embedded in a complex politico-economic setting, we have developed an analytical framework, incorporating both economic, political and societal elements and the interplay between them. More specifically, environmentally oriented innovation is seen as evolving out of the interplay between: 1) a technical-commercial core (major market agents, i.e. the renewable energy supply industry, associated complementary industries and consumers); 2) the political-administrative system; and 3) the societal basis, referring to existing societal customs, norms, and modes of social organisation. Drawing on Porter's (1990) analysis of business clusters, we have termed our focus ''green energy-industrial cluster'' emergence and growth. We have taken Porter's attempt to break out of a limited market analysis into a broader strategic focus one step further, where the political and institutional dimensions are more explicitly included. This implies that we see the emergence of

Green Energy-Industrial Innovation: A Comparative Study of Green Energy Transformations in Northern Europe

International Nuclear Information System (INIS)

Eikeland, Per Ove; Christiansen, Atle Christer; Koefoed, Anne Louise; Midttun, Atle; Tangen, Kristian

1999-01-01

Greening of industry is a prominent topic on the European agenda and has received a new impetus with the Kyoto process, where the European Union and its member states are facing new obligations. Due to its role as a major infrastructure sector and due to its extensive emissions, the electricity sector has become a focal industry in this context. These same factors, as well as the large public ownership has, in fact, traditionally placed energy in a central public political focus. The energy and electricity sector hence exemplify the intertwined nature of economics and politics in sector developments, where technology choices and capacity expansions have numerous motivations and diverse interests to please. This report discusses ''green'' innovation in the energy industry. By selecting some of the most advanced examples of breakthrough for new green technologies in Europe, it explores the basic elements of successful greening of industry. To account for the fact that product innovation- and diffusion-processes are embedded in a complex politico-economic setting, we have developed an analytical framework, incorporating both economic, political and societal elements and the interplay between them. More specifically, environmentally oriented innovation is seen as evolving out of the interplay between: 1) a technical-commercial core (major market agents, i.e. the renewable energy supply industry, associated complementary industries and consumers); 2) the political-administrative system; and 3) the societal basis, referring to existing societal customs, norms, and modes of social organisation. Drawing on Porter's (1990) analysis of business clusters, we have termed our focus ''green energy-industrial cluster'' emergence and growth. We have taken Porter's attempt to break out of a limited market analysis into a broader strategic focus one step further, where the political and institutional dimensions are more explicitly included. This implies that we see the emergence of

Association between VDAC1 mRNA expression and intracellular ...

African Journals Online (AJOL)

ELO

2012-01-05

Jan 5, 2012 ... generate reactive oxygen species (ROS) and oxidative stress. Unchecked ... PCR Master Mix, SYBR Green-Real Master Mix, and Quantscript ... had reached 70%, they were exposed to Cr(VI) for different time periods (12, 24 ...

Shear bond strength of one-step self-etch adhesives to enamel: effect of acid pretreatment.

Science.gov (United States)

Poggio, Claudio; Scribante, Andrea; Della Zoppa, Federica; Colombo, Marco; Beltrami, Riccardo; Chiesa, Marco

2014-02-01

The purposes of this study were to evaluate the effect of surface pretreatment with phosphoric acid on the enamel bond strength of four-one-step self-etch adhesives with different pH values. One hundred bovine permanent mandibular incisors were used. The materials used in this study included four-one-step self-etch adhesives with different pH values: Adper(™) Easy Bond Self-Etch Adhesive (ph = 0,8-1), Futurabond NR (ph = 1,4), G-aenial Bond (ph = 1,5), Clearfil(3) S Bond (ph = 2,7). One two-step self-etch adhesive (Clearfil SE Bond/ph = 0,8-1) was used as control. The teeth were assigned into two subgroups according to bonding procedure. In the first subgroup (n = 50), no pretreatment agent was applied. In the second subgroup (n = 50), etching was performed using 37% phosphoric acid for 30 s. After adhesive systems application, a nanohybrid composite resin was inserted into the enamel surface. The specimens were placed in a universal testing machine (Model 3343, Instron Corp., Canton, Mass., USA). After the testing procedure, the fractured surfaces were examined with an optical microscope at a magnification of 10× to determine failure modes. The adhesive remnant index (ARI) was used to assess the amount of adhesive left on the enamel surface. Descriptive statistics of the shear bond strength and frequency distribution of ARI scores were calculated. Enamel pretreatment with phosphoric acid significantly increased bond strength values of all the adhesives tested. No significant differences in bond strength were detected among the four different one-step self-etch adhesives with different pH. Two-step self-etch adhesive showed the highest bond strength. © 2013 John Wiley & Sons A/S.

A new one-step procedure for pulmonary valve implantation of the melody valve: Simultaneous prestenting and valve implantation.

Science.gov (United States)

Boudjemline, Younes

2018-01-01

To describe a new modification, the one-step procedure, that allows interventionists to pre-stent and implant a Melody valve simultaneously. Percutaneous pulmonary valve implantation (PPVI) is the standard of care for managing patients with dysfunctional right ventricular outflow tract, and the approach is standardized. Patients undergoing PPVI using the one-step procedure were identified in our database. Procedural data and radiation exposure were compared to those in a matched group of patients who underwent PPVI using the conventional two-step procedure. Between January 2016 and January 2017, PPVI was performed in 27 patients (median age/range, 19.1/10-55 years) using the one-step procedure involving manual crimping of one to three bare metal stents over the Melody valve. The stent and Melody valve were delivered successfully using the Ensemble delivery system. No complications occurred. All patients had excellent hemodynamic results (median/range post-PPVI right ventricular to pulmonary artery gradient, 9/0-20 mmHg). Valve function was excellent. Median procedural and fluoroscopic times were 56 and 10.2 min, respectively, which significantly differed from those of the two-step procedure group. Similarly, the dose area product (DAP), and radiation time were statistically lower in the one-step group than in the two-step group (P step procedure is a safe modification that allows interventionists to prestent and implants the Melody valve simultaneously. It significantly reduces procedural and fluoroscopic times, and radiation exposure. © 2017 Wiley Periodicals, Inc.

Porter Takes Reins of the FNL Green Team | Poster

Science.gov (United States)

Courtesy of the FNL Green Team Melissa Porter, who recently joined the staff of Craig Reynolds, Ph.D., director, Office of Scientific Operations, as administrative manager, has stepped forward to lead the Frederick National Laboratory for Cancer Research (FNL) Green Team in its efforts to promote a “green” work environment. “I am excited to lead the FNL Green Team and have

A simple one-step chemistry model for partially premixed hydrocarbon combustion

Energy Technology Data Exchange (ETDEWEB)

Fernandez-Tarrazo, Eduardo [Instituto Nacional de Tecnica Aeroespacial, Madrid (Spain); Sanchez, Antonio L. [Area de Mecanica de Fluidos, Universidad Carlos III de Madrid, Leganes 28911 (Spain); Linan, Amable [ETSI Aeronauticos, Pl. Cardenal Cisneros 3, Madrid 28040 (Spain); Williams, Forman A. [Department of Mechanical and Aerospace Engineering, University of California San Diego, La Jolla, CA 92093-0411 (United States)

2006-10-15

This work explores the applicability of one-step irreversible Arrhenius kinetics with unity reaction order to the numerical description of partially premixed hydrocarbon combustion. Computations of planar premixed flames are used in the selection of the three model parameters: the heat of reaction q, the activation temperature T{sub a}, and the preexponential factor B. It is seen that changes in q with equivalence ratio f need to be introduced in fuel-rich combustion to describe the effect of partial fuel oxidation on the amount of heat released, leading to a universal linear variation q(f) for f>1 for all hydrocarbons. The model also employs a variable activation temperature T{sub a}(f) to mimic changes in the underlying chemistry in rich and very lean flames. The resulting chemistry description is able to reproduce propagation velocities of diluted and undiluted flames accurately over the whole flammability limit. Furthermore, computations of methane-air counterflow diffusion flames are used to test the proposed chemistry under nonpremixed conditions. The model not only predicts the critical strain rate at extinction accurately but also gives near-extinction flames with oxygen leakage, thereby overcoming known predictive limitations of one-step Arrhenius kinetics. (author)

One-Step Nickel Foam Assisted Synthesis of Holey G-Carbon Nitride Nanosheets for Efficient Visible-light Photocatalytic H2 Evolution.

Science.gov (United States)

Fang, Zhenyuan; Hong, Yuanzhi; Li, Di; Luo, Bifu; Mao, Baodong; Shi, Weidong

2018-06-01

Graphitic carbon nitride (g-C3N4) with layered structure represents one of the most promising metal-free photocatalysts. As yet, the direct one-step synthesis of ultrathin g-C3N4 nanosheets remains a challenge. Here, few-layered holey g-C3N4 nanosheets (CNS) were fabricated by simply introducing a piece of nickel foam over the precursors during the heating process. The as-prepared CNS with unique structural advantages exhibited superior photocatalytic water splitting activity (1871.09 µmol h-1 g-1) than bulk g-C3N4 (BCN) under visible light (λ>420 nm) (≈31 fold). Its outstanding photocatalytic performance originated from the high specific surface area (240.34 m2 g-1) and mesoporous structure, which endows CNS with more active sites, efficient exciton dissociation and prolonged charge carrier lifetime. Moreover, the obvious up-shift of the conduction band leads to a larger thermodynamic driving force for photocatalytic proton reduction. This methodology not only had the advantages for the direct and green synthesis of g-C3N4 nanosheets, but also paved a new avenue to modify molecular structure and textural of g-C3N4 for advanced applications.

Bond strength of one-step self-etch adhesives and their predecessors to ground versus unground enamel.

Science.gov (United States)

Yazici, A Ruya; Yildirim, Zeren; Ertan, Atila; Ozgunaltay, Gül; Dayangac, Berrin; Antonson, Sibel A; Antonson, Donald E

2012-07-01

The aim of this study was to compare the shear bond strength of several self-etch adhesives to their two-step predecessors to ground and unground enamel. Seventy-five extracted, non-carious human third molar teeth were selected for this study. The buccal surfaces of each tooth were mechanically ground to obtain flat enamel surfaces (ground enamel), while the lingual surfaces were left intact (unground enamel). The teeth were randomly divided into five groups according to the adhesive systems (n=15): one-step self-etch adhesive - Clearfil S3 Bond, its two-step predecessor - Clearfil SE Bond, one-step self-etch adhesive - AdheSE One, and its two-step predecessor - AdheSE, and a two-step etch-and-rinse adhesive - Adper Single Bond 2(control). After application of the adhesives to the buccal and lingual enamel surfaces of each tooth, a cylindrical capsule filled with a hybrid composite resin (TPH) was seated against the surfaces. The specimens were stored in distilled water at 37°C for 24 hours, followed by thermocy-cling (5°C-55°C/500 cycles). They were subjected to shear bond strength test in a universal testing machine at a crosshead speed of 1.0 mm/minute. The data were compared using a two-way ANOVA, followed by Bonferroni test at Padhesives exhibited statistically similar bond strengths to ground and unground enamel except for the etch-and-rinse adhesive that showed significantly higher bond strengths than the self-etch adhesives (Padhesives tested (P=.17). Similar bond strengths to ground and unground enamel were achieved with one-step self-etch adhesives and their predecessors. Enamel preparation did not influence the bonding performance of the adhesives tested.

Bond strength of one-step self-etch adhesives and their predecessors to ground versus unground enamel

Science.gov (United States)

Yazici, A. Ruya; Yildirim, Zeren; Ertan, Atila; Ozgunaltay, Gül; Dayangac, Berrin; Antonson, Sibel A; Antonson, Donald E

2012-01-01

Objective The aim of this study was to compare the shear bond strength of several self-etch adhesives to their two-step predecessors to ground and unground enamel. Methods: Seventy-five extracted, non-carious human third molar teeth were selected for this study. The buccal surfaces of each tooth were mechanically ground to obtain flat enamel surfaces (ground enamel), while the lingual surfaces were left intact (unground enamel). The teeth were randomly divided into five groups according to the adhesive systems (n=15): one-step self-etch adhesive - Clearfil S3 Bond, its two-step predecessor - Clearfil SE Bond, one-step self-etch adhesive - AdheSE One, and its two-step predecessor - AdheSE, and a two-step etch-and-rinse adhesive - Adper Single Bond 2(control). After application of the adhesives to the buccal and lingual enamel surfaces of each tooth, a cylindrical capsule filled with a hybrid composite resin (TPH) was seated against the surfaces. The specimens were stored in distilled water at 37°C for 24 hours, followed by thermocy-cling (5°C–55°C/500 cycles). They were subjected to shear bond strength test in a universal testing machine at a crosshead speed of 1.0 mm/minute. The data were compared using a two-way ANOVA, followed by Bonferroni test at Padhesives exhibited statistically similar bond strengths to ground and unground enamel except for the etch-and-rinse adhesive that showed significantly higher bond strengths than the self-etch adhesives (Penamel for any of the adhesives tested (P=.17). Conclusion: Similar bond strengths to ground and unground enamel were achieved with one-step self-etch adhesives and their predecessors. Enamel preparation did not influence the bonding performance of the adhesives tested. PMID:22904656

Green colorants based on energetic azole borates.

Science.gov (United States)

Glück, Johann; Klapötke, Thomas M; Rusan, Magdalena; Stierstorfer, Jörg

2014-11-24

The investigation of green-burning boron-based compounds as colorants in pyrotechnic formulations as alternative for barium nitrate, which is a hazard to health and to the environment, is reported. Metal-free and nitrogen-rich dihydrobis(5-aminotetrazolyl)borate salts and dihydrobis(1,3,4-triazolyl)borate salts have been synthesized and characterized by NMR spectroscopy, elemental analysis, mass spectrometry, and vibrational spectroscopy. Their thermal and energetic properties have been determined as well. Several pyrotechnic compositions using selected azolyl borate salts as green colorants were investigated. Formulations with ammonium dinitramide and ammonium nitrate as oxidizers and boron and magnesium as fuels were tested. The burn time, dominant wavelength, spectral purity, luminous intensity, and luminous efficiency as well as the thermal and energetic properties of these compositions were measured. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Will learning to solve one-step equations pose a challenge to 8th grade students?

Science.gov (United States)

Ngu, Bing Hiong; Phan, Huy P.

2017-08-01

Assimilating multiple interactive elements simultaneously in working memory to allow understanding to occur, while solving an equation, would impose a high cognitive load. Element interactivity arises from the interaction between elements within and across operational and relational lines. Moreover, operating with special features (e.g. negative pronumeral) poses additional challenge to master equation solving skills. In an experiment, 41 8th grade students (girls = 16, boys = 25) sat for a pre-test, attended a session about equation solving, completed an acquisition phase which constituted the main intervention and were tested again in a post-test. The results showed that at post-test, students performed better on one-step equations tapping low rather than high element interactivity knowledge. In addition, students performed better on those one-step equations that contained no special features. Thus, both the degree of element interactivity and the operation with special features affect the challenge posed to 8th grade students on learning how to solve one-step equations.

One-step leapfrog ADI-FDTD method for simulating electromagnetic wave propagation in general dispersive media.

Science.gov (United States)

Wang, Xiang-Hua; Yin, Wen-Yan; Chen, Zhi Zhang David

2013-09-09

The one-step leapfrog alternating-direction-implicit finite-difference time-domain (ADI-FDTD) method is reformulated for simulating general electrically dispersive media. It models material dispersive properties with equivalent polarization currents. These currents are then solved with the auxiliary differential equation (ADE) and then incorporated into the one-step leapfrog ADI-FDTD method. The final equations are presented in the form similar to that of the conventional FDTD method but with second-order perturbation. The adapted method is then applied to characterize (a) electromagnetic wave propagation in a rectangular waveguide loaded with a magnetized plasma slab, (b) transmission coefficient of a plane wave normally incident on a monolayer graphene sheet biased by a magnetostatic field, and (c) surface plasmon polaritons (SPPs) propagation along a monolayer graphene sheet biased by an electrostatic field. The numerical results verify the stability, accuracy and computational efficiency of the proposed one-step leapfrog ADI-FDTD algorithm in comparison with analytical results and the results obtained with the other methods.

Recombinant Passenger Proteins Can Be Conveniently Purified by One-Step Affinity Chromatography.

Science.gov (United States)

Wang, Hua-zhen; Chu, Zhi-zhan; Chen, Chang-chao; Cao, Ao-cheng; Tong, Xin; Ouyang, Can-bin; Yuan, Qi-hang; Wang, Mi-nan; Wu, Zhong-kun; Wang, Hai-hong; Wang, Sheng-bin

2015-01-01

Fusion tag is one of the best available tools to date for enhancement of the solubility or improvement of the expression level of recombinant proteins in Escherichia coli. Typically, two consecutive affinity purification steps are often necessitated for the purification of passenger proteins. As a fusion tag, acyl carrier protein (ACP) could greatly increase the soluble expression level of Glucokinase (GlcK), α-Amylase (Amy) and GFP. When fusion protein ACP-G2-GlcK-Histag and ACP-G2-Amy-Histag, in which a protease TEV recognition site was inserted between the fusion tag and passenger protein, were coexpressed with protease TEV respectively in E. coli, the efficient intracellular processing of fusion proteins was achieved. The resulting passenger protein GlcK-Histag and Amy-Histag accumulated predominantly in a soluble form, and could be conveniently purified by one-step Ni-chelating chromatography. However, the fusion protein ACP-GFP-Histag was processed incompletely by the protease TEV coexpressed in vivo, and a large portion of the resulting target protein GFP-Histag aggregated in insoluble form, indicating that the intracellular processing may affect the solubility of cleaved passenger protein. In this context, the soluble fusion protein ACP-GFP-Histag, contained in the supernatant of E. coli cell lysate, was directly subjected to cleavage in vitro by mixing it with the clarified cell lysate of E. coli overexpressing protease TEV. Consequently, the resulting target protein GFP-Histag could accumulate predominantly in a soluble form, and be purified conveniently by one-step Ni-chelating chromatography. The approaches presented here greatly simplify the purification process of passenger proteins, and eliminate the use of large amounts of pure site-specific proteases.

One-particle many-body Green's function theory: Algebraic recursive definitions, linked-diagram theorem, irreducible-diagram theorem, and general-order algorithms.

Science.gov (United States)

Hirata, So; Doran, Alexander E; Knowles, Peter J; Ortiz, J V

2017-07-28

A thorough analytical and numerical characterization of the whole perturbation series of one-particle many-body Green's function (MBGF) theory is presented in a pedagogical manner. Three distinct but equivalent algebraic (first-quantized) recursive definitions of the perturbation series of the Green's function are derived, which can be combined with the well-known recursion for the self-energy. Six general-order algorithms of MBGF are developed, each implementing one of the three recursions, the ΔMPn method (where n is the perturbation order) [S. Hirata et al., J. Chem. Theory Comput. 11, 1595 (2015)], the automatic generation and interpretation of diagrams, or the numerical differentiation of the exact Green's function with a perturbation-scaled Hamiltonian. They all display the identical, nondivergent perturbation series except ΔMPn, which agrees with MBGF in the diagonal and frequency-independent approximations at 1≤n≤3 but converges at the full-configuration-interaction (FCI) limit at n=∞ (unless it diverges). Numerical data of the perturbation series are presented for Koopmans and non-Koopmans states to quantify the rate of convergence towards the FCI limit and the impact of the diagonal, frequency-independent, or ΔMPn approximation. The diagrammatic linkedness and thus size-consistency of the one-particle Green's function and self-energy are demonstrated at any perturbation order on the basis of the algebraic recursions in an entirely time-independent (frequency-domain) framework. The trimming of external lines in a one-particle Green's function to expose a self-energy diagram and the removal of reducible diagrams are also justified mathematically using the factorization theorem of Frantz and Mills. Equivalence of ΔMPn and MBGF in the diagonal and frequency-independent approximations at 1≤n≤3 is algebraically proven, also ascribing the differences at n = 4 to the so-called semi-reducible and linked-disconnected diagrams.

One-step aqueous synthesis of fluorescent copper nanoclusters by direct metal reduction

International Nuclear Information System (INIS)

Fernández-Ujados, Mónica; Trapiella-Alfonso, Laura; Costa-Fernández, José M; Pereiro, Rosario; Sanz-Medel, Alfredo

2013-01-01

A one-step aqueous synthesis of highly fluorescent water-soluble copper nanoclusters (CuNCs) is here described, based on direct reduction of the metal precursor with NaBH 4 in the presence of bidentate ligands (made of lipoic acid anchoring groups, appended with a poly(ethylene glycol) short chain). A complete optical and structural characterization was carried out: the optical emission was centred at 416 nm, with a luminescence quantum yield in water of 3.6% (the highest one reported so far in water for this kind of nanocluster). The structural characterization reveals a homogeneous size distribution (of 2.5 nm diameter) with spherical shape. The CuNCs obtained offer long-term stability (the luminescence emission remained unaltered after more than two months) under a broad range of chemical conditions (e.g. stored at pH 3–12 or even in a high ionic strength medium such as 1 M NaCl) and high photostability, keeping their fluorescence emission intact after more than 2 h of daylight and UV-light exposition. All those advantageous features warrant synthesized CuNCs being promising fluorescent nanoprobes for further developments including (bio)applications. (paper)

Green Application for Space Power

Science.gov (United States)

Robinson, Joel

2015-01-01

Most space vehicle auxiliary power units (APUs) use hydrazine propellant for generating power. Hydrazine is a toxic, hazardous fuel that requires special safety equipment and processes for handling and loading. In recent years, there has been development of two green propellants (less toxic) that could enable their use in APUs. The Swedish government, in concert with the Swedish Space Corporation, has developed a propellant based on ammonium dinitramide (LMP-103S) that was flown on the Prisma spacecraft in 2010. The United States Air Force (USAF) has been developing a propellant based on hydroxylammonium nitrate (AFM315E) that is scheduled to fly on the Green Propellant Infusion Mission in the spring of 2016 to demonstrate apogee and reaction control thrusters. However, no one else in the Agency is currently pursuing use of green propellants for application to the APUs. Per the TA-01 Launch Propulsion Roadmap, the Space Technology Mission Directorate had identified the need to have a green propellant APU by 2015. This is our motivation for continuing activities.

Towards green loyalty: the influences of green perceived risk, green image, green trust and green satisfaction

Science.gov (United States)

Chrisjatmiko, K.

2018-01-01

The paper aims to present a comprehensive framework for the influences of green perceived risk, green image, green trust and green satisfaction to green loyalty. The paper also seeks to account explicitly for the differences in green perceived risk, green image, green trust, green satisfaction and green loyalty found among green products customers. Data were obtained from 155 green products customers. Structural equation modeling was used in order to test the proposed hypotheses. The findings show that green image, green trust and green satisfaction has positive effects to green loyalty. But green perceived risk has negative effects to green image, green trust and green satisfaction. However, green perceived risk, green image, green trust and green satisfaction also seems to be a good device to gain green products customers from competitors. The contributions of the paper are, firstly, a more complete framework of the influences of green perceived risk, green image, green trust and green satisfaction to green loyalty analyses simultaneously. Secondly, the study allows a direct comparison of the difference in green perceived risk, green image, green trust, green satisfaction and green loyalty between green products customers.

Green maritime transportation: Market based measures

DEFF Research Database (Denmark)

Psaraftis, Harilaos N.

2016-01-01

The purpose of this chapter is to introduce the concept of Market Based Measures (MBMs) to reduce Green House Gas (GHG) emissions from ships, and review several distinct MBM proposals that have been under consideration by the International Maritime Organization (IMO). The chapter discusses the me...... the mechanisms used by MBMs, and explores how the concept of the Marginal Abatement Cost (MAC) can be linked to MBMs. It also attempts to discuss the pros and cons of the submitted proposals....

One-Step Generation of Multi-Qubit GHZ and W States in Superconducting Transmon Qubit System

International Nuclear Information System (INIS)

Gao Guilong; Huang Shousheng; Wang Mingfeng; Jiang Nianquan; Cai Genchang

2012-01-01

We propose a one-step method to prepare multi-qubit GHZ and W states with transmon qubits capacitively coupled to a superconducting transmission line resonator (TLR). Compared with the scheme firstly introduced by Wang et al. [Phys. Rev. B 81 (2010) 104524], our schemes have longer dephasing time and much shorter operation time because the transmon qubits we used are not only more robust to the decoherence and the unavoidable parameter variations, but also have much stronger coupling constant with TLR. Based on the favourable properties of transmons and TLR, our method is more feasible in experiment. (general)

In situ biosynthesis of bacterial nanocellulose-CaCO3 hybrid bionanocomposite: One-step process

International Nuclear Information System (INIS)

Mohammadkazemi, Faranak; Faria, Marisa; Cordeiro, Nereida

2016-01-01

In this work, a simple and green route to the synthesis of the bacterial nanocellulose-calcium carbonate (BNC/CaCO 3 ) hybrid bionanocomposites using one-step in situ biosynthesis was studied. The CaCO 3 was incorporated in the bacterial nanocellulose structure during the cellulose biosynthesis by Gluconacetobacter xylinus PTCC 1734 bacteria. Hestrin-Schramm (HS) and Zhou (Z) culture media were used to the hybrid bionanocomposites production and the effect of ethanol addition was investigated. Attenuated total reflection Fourier transform infrared spectroscopy, field emission scanning electron microscopy, X-ray diffraction, energy-dispersive X-ray spectroscopy, inverse gas chromatography and thermogravimetric analysis were used to characterize the samples. The experimental results demonstrated that the ethanol and culture medium play an important role in the BNC/CaCO 3 hybrid bionanocomposites production, structure and properties. The BNC/CaCO 3 biosynthesized in Z culture medium revealed higher O/C ratio and amphoteric surface character, which justify the highest CaCO 3 content incorporation. The CaCO 3 was incorporated into the cellulosic matrix decreasing the bacterial nanocellulose crystallinity. This work reveals the high potential of in situ biosynthesis of BNC/CaCO 3 hybrid bionanocomposites and opens a new way to the high value-added applications of bacterial nanocellulose. - Graphical Abstract: Display Omitted - Highlights: • BNC/CaCO 3 hybrid bionanocomposites were produced using in situ biosynthesis process. • Ethanol and culture medium play an important role in the production and properties. • Z-BNC/CaCO 3 bionanocomposites revealed higher O/C ratio and amphoteric surface character. • CaCO 3 incorporated into the BNC decreased crystallinity.

One False Step: "Detroit," "Step" and Movies of Rising and Falling

Science.gov (United States)

Beck, Bernard

2018-01-01

"Detroit" and "Step" are two recent movies in the context of urban riots in protest of police brutality. They refer to time periods separated by half a century, but there are common themes in the two that seem appropriate to both times. The movies are not primarily concerned with the riot events, but the riot is a major…

Development of a Real-time PCR test for porcine group A rotavirus diagnosis

OpenAIRE

Marconi, Elizabeth C.M.; Bernardes, Nara T.C.G.; Beserra, Laila A.R.; Silva, Fernanda D.F.; Gregori, Fabio

2015-01-01

Group A Rotavirus (RVA) is one of the most common causes of diarrhea in humans and several animal species. A SYBR-Green Real-Time polymerase chain reaction (PCR) was developed to diagnose RVA from porcine fecal samples, targeting amplification of a 137-bp fragment of nonstructural protein 5 (NSP5) gene using mRNA of bovine NADH-desidrogenase-5 as exogenous internal control. Sixty-five samples were tested (25 tested positive for conventional PCR and genetic sequencing). The overall agreement (...

One-step synthesis of graphene-Pt nanocomposites by gamma-ray irradiation

International Nuclear Information System (INIS)

Tokai, Akihiro; Okitsu, Kenji; Hori, Fuminobu; Mizukoshi, Yoshiteru; Iwase, Akihiro

2016-01-01

We developed a one-step gamma-ray irradiation method to synthesize nanocomposites composed of graphene and Pt nanoparticles from aqueous solution containing graphene and Pt(IV) complex ions in the presence of 2-propanol (IPA) or sodium dodecyl sulfate (SDS). It was confirmed that gamma-ray irradiation provided carbonyl groups on graphene and Pt nanoparticles formed from the radiolytic reduction of Pt(IV) complex ions were deposited onto the carbonyl modified graphene. In the presence of IPA, small Pt nanoparticles were deposited on graphene, but large Pt nanoparticles were deposited in the presence of SDS: the size of Pt nanoparticles formed was larger in the presence of SDS than IPA. Based on the results, formation and deposition mechanisms of Pt nanoparticles were proposed. - Highlights: • Graphene-Pt nanocomposites were synthesized by gamma-ray irradiation. • Reduction of Pt(IV) complex ions and oxidation of graphene occurred simultaneously. • Smaller Pt nanoparticles were formed in the presence of IPA than SDS. • Mechanism for formation of graphene-Pt nanocomposites was proposed.

Accessibility of green space in urban areas: an examination of various approaches to measure it

OpenAIRE

Zhang, Xin

2007-01-01

In the present research, we attempt to improve the methods used for measuring accessibility of green spaces by combining two components of accessibility-distance and demand relative to supply. Three modified approaches (Joseph and Bantock gravity model measure, the two-step floating catchment area measure and a measure based on kernel densities) will be applied for measuring accessibility to green spaces. We select parks and public open spaces (metropolitan open land) of south London as a cas...

Dose Rate Experiment at JET for Benchmarking the Calculation Direct One Step Method

International Nuclear Information System (INIS)

Angelone, M.; Petrizzi, L.; Pillon, M.; Villari, R.; Popovichev, S.

2006-01-01

Neutrons produced by D-D and D-T plasmas induce the activation of tokamak materials and of components. The development of reliable methods to assess dose rates is a key issue for maintenance and operating nuclear machines, in normal and off-normal conditions. In the frame of the EFDA Fusion Technology work programme, a computational tool based upon MCNP Monte Carlo code has been developed to predict the dose rate after shutdown: it is called Direct One Step Method (D1S). The D1S is an innovative approach in which the decay gammas are coupled to the neutrons as in the prompt case and they are transported in one single step in the same run. Benchmarking of this new tool with experimental data taken in a complex geometry like that of a tokamak is a fundamental step to test the reliability of the D1S method. A dedicated benchmark experiment was proposed for the 2005-2006 experimental campaign of JET. Two irradiation positions have been selected for the benchmark: one inner position inside the vessel, not far from the plasma, called the 2 upper irradiation end (IE2), where neutron fluence is relatively high. The second position is just outside a vertical port in an external position (EX). Here the neutron flux is lower and the dose rate to be measured is not very far from the residual background. Passive detectors are used for in-vessel measurements: the high sensitivity Thermo Luminescent Dosimeters (TLDs) GR-200A (natural LiF), which ensure measurements down to environmental dose level. An active detector of Geiger-Muller (GM) type is used for out of vessel dose rate measurement. Before their use the detectors were calibrated in a secondary gamma-ray standard (Cs-137 and Co-60) facility in term of air-kerma. The background measurement was carried-out in the period July -September 2005 in the outside position EX using the GM tube and in September 2005 inside the vacuum vessel using TLD detectors located in the 2 Upper irradiation end IE2. In the present work

GREEN APPROACH TO BULK AND TEMPLATE-FREE SYNTHESIS OF THERMALLY STABLE REDUCED POLYANILINE NANOFIBERS FOR CAPACITOR APPLICATIONS

Science.gov (United States)

An extremely simple green approach is described that generates bulk quantities of nanofibers of the electronic polymer polyaniline in fully reduced state (leucoemeraldine form) in one step without using any reducing agent, surfactants, and/or large amounts of insoluble templates....

Green City Branding in Perspective d

DEFF Research Database (Denmark)

Gulsrud, Natalie Marie

2014-01-01

their competitive advantage as an increasingly global economy has led to fierce competition between cities at a national and international level. Cities are actively competing for talent, innovation, and creativity to boost their economies. One way cities achieve a competitive image is through green place branding......From Sydney, Australia’s “Sustainable Sydney 2030” campaign, to Vancouver, Canada’s “Greenest City 2020” vision, green city brands have become a global tool for municipal leaders to promise a better quality of life, promote sustainable development, and increase their competitive advantage. In Asia......, various green city schemes and rankings exist. They include Siemen’s Asian Green City Index, assessed by The Economist Intelligence Unit, based on a city’s environmental performance in a wide range of categories (Economist Intelligence Unit 2011). These green city brands provide a vision of health...

No greens in the forest? Note on the limited consumption of greens in the Amazon

Directory of Open Access Journals (Sweden)

Esther Katz

2012-12-01

Full Text Available The consumption of greens is reported as being very minor among Amazonian Indians. The authors of this article present a new review of this subject, based on fieldwork with Amerindians and other populations in different parts of the Brazilian Amazon and French Guiana. Written sources on Brazilian, Peruvian, Columbian and Venezuelan Amazon were also reviewed. The consumption of cultivated, semi-cultivated and wild species of greens was taken into account here, as the data specific to wild greens is very scarce. It is confirmed that greens are not commonly eaten among native Amazonians and that some ethnic groups do not consume them at all. The consumed species are usually young shoots of weeds or cassava leaves. Common in the Belém region are some specific aromatic plants, which have been diffused to other parts of the Amazon, together with introduced plants such as kale and coriander. Migrants from Northeastern Brazil settled in the Amazon consume some cultivated greens, especially aromatic plants. Maroons are the ones who use more greens in their diet. Native Amazonian people, who supplement agriculture with game and fish, follow a hunter-gatherer pattern, preferring wild fruit and tubers to greens.

One-step FPLC-size-exclusion chromatography procedure for purification of rDMBT1 6 kb with increased biological activity

DEFF Research Database (Denmark)

Tuttolomondo, Martina; Hansen, Pernille Lund; Mollenhauer, Jan

2018-01-01

from saliva or produced in vitro and purified by a multistep affinity purification procedure using bacteria, followed by FPLC. Here, we compared a simple, one-step FPLC-SEC protocol for purification of recombinant DMBT1 6 kb, with that of the standard bacteria affinity purification-based protocol. Our...

On Green's function for 3-D wave-body interaction in a channel

DEFF Research Database (Denmark)

Xia, Jinzhu

1997-01-01

series of images is evaluated accurately based on an asmptotic analysis. It is demonstrated that the Green's function has a square-root singular behaviour due to the side walls when the wave frequency approaches one of the resonant frequencies. The numerical results for the Green's function has a square......An analytical and numerical study is presented for efficient evaluation of the Green's function that satisfies the linear free surface condition and the non-penetration condition on the channel bottomand the side walls. the formulation is based on the open-sea green's function and the complete......-root singular behaviour due to the side walls when the wave frequency approaches one of the resonant frequencies. The numerical results for the Green's funciton presented in the present paper are believed to have an absolute accuracy of 10-5....

Green corridors in freight logistics

DEFF Research Database (Denmark)

Panagakos, George

of the performance of a green corridor in terms of pre-specified Key Performance Indicators (KPIs). The thesis builds on previous own work under the EUfinanced SuperGreen project and applies the new methodology on the GreCOR corridor extending from Oslo to Rotterdam. The scope of the two other objectives relates...... the rationale for a performance monitoring scheme has been established, the thesis critically reviews the SuperGreen methodology which consists of: (i) decomposing the corridor into transport chains, (ii) selecting a sample of typical chains, (iii) assessing these chains through a set of KPIs, and (iv......) aggregating the chain-level KPIs to corridor-level ones using proper weights. Unlike SuperGreen that suggests a study-based approach for constructing the corridor sample, the thesis proposes founding the selection of typical chains on the outcome of specialised transport models. The periodic collection...

Improvement of High-throughput Genotype Analysis After Implementation of a Dual-curve Sybr Green I-based Quantification and Normalization Procedure

Science.gov (United States)

The ability to rapidly screen a large number of individuals is the key to any successful plant breeding program. One of the primary bottlenecks in high throughput screening is the preparation of DNA samples, particularly the quantification and normalization of samples for downstream processing. A ...

One-step synthesis of magnetic chitosan for controlled release of 5-hydroxytryptophan

Energy Technology Data Exchange (ETDEWEB)

Santos Menegucci, Jucély dos; Santos, Mac-Kedson Medeiros Salviano; Dias, Diego Juscelino Santos; Chaker, Juliano Alexandre; Sousa, Marcelo Henrique, E-mail: mhsqui@gmail.com

2015-04-15

In this work, nanoparticles of chitosan embedded with 25% (w/w) of iron oxide magnetic nanoparticles (magnetite/maghemite) with narrow size-distribution and with a loading efficiency of about 80% for 5-hydroxytryptophan (5-HTP), which is a chemical precursor in the biosynthesis of important neurotransmitters as serotonin, were synthesized with an initial mass ratio of 5-HTP/magnetic chitosan=1.2, using homogeneous precipitation by urea decomposition, in an efficient one-step procedure. Characterization of morphology, structure and surface were performed by XRD, TEM, FTIR, TGA, magnetization and zeta potential measurements, while drug loading and drug releasing were investigated using UV–vis spectroscopy. Kinetic drug release experiments under different pH conditions revealed a pH-sensitivecontrolled-release system, ruled by polymer swelling and/or particle dissolution. - Highlights: • One-step synthesis and incorporation of drug in magnetic chitosan. • Synthesis utilizes a cost-effective and environmentally friendly procedure. • Narrow size distribution of magnetic nanoparticles in the composite. • Composite is a basis for a magnetic pH triggered drug release system.

A new deterministic Ensemble Kalman Filter with one-step-ahead smoothing for storm surge forecasting

KAUST Repository

Raboudi, Naila

2016-11-01

The Ensemble Kalman Filter (EnKF) is a popular data assimilation method for state-parameter estimation. Following a sequential assimilation strategy, it breaks the problem into alternating cycles of forecast and analysis steps. In the forecast step, the dynamical model is used to integrate a stochastic sample approximating the state analysis distribution (called analysis ensemble) to obtain a forecast ensemble. In the analysis step, the forecast ensemble is updated with the incoming observation using a Kalman-like correction, which is then used for the next forecast step. In realistic large-scale applications, EnKFs are implemented with limited ensembles, and often poorly known model errors statistics, leading to a crude approximation of the forecast covariance. This strongly limits the filter performance. Recently, a new EnKF was proposed in [1] following a one-step-ahead smoothing strategy (EnKF-OSA), which involves an OSA smoothing of the state between two successive analysis. At each time step, EnKF-OSA exploits the observation twice. The incoming observation is first used to smooth the ensemble at the previous time step. The resulting smoothed ensemble is then integrated forward to compute a "pseudo forecast" ensemble, which is again updated with the same observation. The idea of constraining the state with future observations is to add more information in the estimation process in order to mitigate for the sub-optimal character of EnKF-like methods. The second EnKF-OSA "forecast" is computed from the smoothed ensemble and should therefore provide an improved background. In this work, we propose a deterministic variant of the EnKF-OSA, based on the Singular Evolutive Interpolated Ensemble Kalman (SEIK) filter. The motivation behind this is to avoid the observations perturbations of the EnKF in order to improve the scheme\\'s behavior when assimilating big data sets with small ensembles. The new SEIK-OSA scheme is implemented and its efficiency is demonstrated

Green space branding in Denmark in an era of neoliberal governance

DEFF Research Database (Denmark)

Gulsrud, Natalie Marie; Gooding, Saskia; Konijnendijk, Cecil Cornelis

2013-01-01

City place branding, an entrepreneurial urban development scheme, aims to differentiate cities from their national and international competitors based on strengths and competitive advantage. One such strength is quality urban green space which has been shown to make cities more attractive...... and liveable places, drawing people and investments to urban centres. Applying a place branding approach, this paper presents the results of a survey of Danish municipalities and their place branding in terms of crafting green city, or environmentally sustainable, profiles. Based on survey responses from both...... municipal green space and communication staff, an overview is presented of the status of `green' municipal place branding, with emphasis on branding through green spaces such as parks. Findings show that green concepts such as environmentally sustainable policies as well as biophysical assets such as green...

One-step femtosecond laser welding and internal machining of three glass substrates

Science.gov (United States)

Tan, Hua; Duan, Ji'an

2017-05-01

In this paper, it demonstrated one-step femtosecond laser welding and internal machining of three fused silica substrates in the optical- and non-optical-contact regimes by focusing 1030-nm laser pulses at the middle of the second substrate. Focusing laser pulses within the second glass in optical-contact and non-optical-contact samples induces permanent internal structural modification, leading to the three glass substrates bonding together simultaneously. The bonding mechanism is based on the internal modification of glass, and this mechanism is different from that of ordinary glass welding at the interface. Welding-spot size is affected by not only the gap distance (ablation effect) and heat transmission, but also by gravity through examining the sizes of the welding spots on the four contact welding surfaces. The maximum bonding strength of the lower interface (56.2 MPa) in the optical-contact regime is more than double that (27.6 MPa) in the non-optical-contact regime.

Study of changes in bacterial and viral abundance in formaldehyde - Fixed water samples by epifluorescence microscopy

Digital Repository Service at National Institute of Oceanography (India)

Parvathi, A.; Radhakrishnan, S.; Sajila, M.P.; Jacob, B.

of bacteria and viruses in water samples from Cochin Backwater was determined by SYBR Green I staining and epifluorescence microscopy. The counts were determined for 45 days in samples fixed with 1–6% formaldehyde. The results suggest rapid decline in counts...

Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data

NARCIS (Netherlands)

Ramakers, Christian; Ruijter, Jan M.; Deprez, Ronald H. Lekanne; Moorman, Antoon F. M.

2003-01-01

Quantification of mRNAs using real-time polymerase chain reaction (PCR) by monitoring the product formation with the fluorescent dye SYBR Green I is being extensively used in neurosciences, developmental biology, and medical diagnostics. Most PCR data analysis procedures assume that the PCR

Cooverexpression of EpCAM and c-myc genes in malignant breast ...

Indian Academy of Sciences (India)

SAMIRA SADEGHI

Samira Sadeghi et al. domain protein2), and the transcription factor Lef1 that is ... antibody approved in. European Market in 2009, to reduce the rate of metastasis in .... Real-Time PCR. System and Maxima SYBR Green/ROX qPCR Master Mix.

A time-dependent Green's function-based model for stream ...

African Journals Online (AJOL)

DRINIE

2003-07-03

Jul 3, 2003 ... applications, this Green's function has found use primarily in linear heat transfer and flow ... based on the mathematical description of the flow with the nonlinear .... i∂/∂x + j∂/∂y is the two-dimensional gradient operator,.

Recyclable crosslinked polymer networks with full property recovery made via one-step controlled radical polymerization

Science.gov (United States)

Jin, Kailong; Li, Lingqiao; Torkelson, John

Rubber tires illustrate well the issues ranging from economic loss to environmental problems and sustainability issues that arise with spent, covalently crosslinked polymers. A nitroxide-mediated polymerization (NMP) strategy has been developed that allows for one-step synthesis of recyclable crosslinked polymers from monomers or polymers that contain carbon-carbon double bonds amenable to radical polymerization. Resulting materials possess dynamic alkoxyamine crosslinks that undergo reversible decrosslinking as a function of temperature. Using polybutadiene as starting material, and styrene, an appropriate nitroxide molecule and bifunctional initiator for initial crosslinking, a model for tire rubber can be produced by reaction at temperatures comparable to those employed in tire molding. Upon cooling, the crosslinks are made permanent due to the extraordinarily strong temperature dependence of the reverisible nitroxide capping and uncapping reaction. Based on thermomechanical property characterization, when the original crosslinked model rubber is chopped into bits and remolded in the melt state, a well-consolidated material is obtained which exhibits full recovery of properties reflecting crosslink density after multiple recycling steps.

One-Step Hydrothermal-Electrochemical Route to Carbon-Stabilized Anatase Powders

Science.gov (United States)

Tao, Ying; Yi, Danqing; Zhu, Baojun

2013-04-01

Black carbon-stabilized anatase particles were prepared by a simple one-step hydrothermal-electrochemical method using glucose and titanium citrate as the carbon and titanium source, respectively. Morphological, chemical, structural, and electrochemical characterizations of these powders were carried out by Raman spectroscopy, Fourier-transform infrared spectroscopy, x-ray diffraction, scanning electron microscopy, and cyclic voltammetry. It was revealed that 200-nm carbon/anatase TiO2 was homogeneously dispersed, and the powders exhibited excellent cyclic performance at high current rates of 0.05 V/s. The powders are interesting potential materials that could be used as anodes for lithium-ion batteries.

A 3-Step Algorithm Using Region-Based Active Contours for Video Objects Detection

Directory of Open Access Journals (Sweden)

Stéphanie Jehan-Besson

2002-06-01

Full Text Available We propose a 3-step algorithm for the automatic detection of moving objects in video sequences using region-based active contours. First, we introduce a very full general framework for region-based active contours with a new Eulerian method to compute the evolution equation of the active contour from a criterion including both region-based and boundary-based terms. This framework can be easily adapted to various applications, thanks to the introduction of functions named descriptors of the different regions. With this new Eulerian method based on shape optimization principles, we can easily take into account the case of descriptors depending upon features globally attached to the regions. Second, we propose a 3-step algorithm for detection of moving objects, with a static or a mobile camera, using region-based active contours. The basic idea is to hierarchically associate temporal and spatial information. The active contour evolves with successively three sets of descriptors: a temporal one, and then two spatial ones. The third spatial descriptor takes advantage of the segmentation of the image in intensity homogeneous regions. User interaction is reduced to the choice of a few parameters at the beginning of the process. Some experimental results are supplied.

Green Energy for Green Economy: The Case Study of Kurdistan Region of Iraq (KRI

Directory of Open Access Journals (Sweden)

Ahmet Sekreter

2017-12-01

Full Text Available Green economy is an overarching purpose for sustainable growth and friendly environment. Renewable energy focuses on clean energy and sustainable development targets a continuous growth. Green economy includes both of them. Kurdistan Region of Iraq (KRI struggles serious problems in terms of economics after showing a remarkable economic growth until mid-2014. The increasing gap between demand and supply is seen another serious problem for KRI. Green energy is one of the essential stage towards to the green economy and it is one of the vital issue to succeed on the way of green economy. Solar energy is one of the fastest growing renewable energy source around the world and KRI has a great potential for solar energy. This study aims to stimulate KRI to invest green energy and encourage it to establish green economy to make its economy robust for the shocks and enable to show a sustainable development.

One-Step Borylation of 1,3-Diaryloxybenzenes Towards Efficient Materials for Organic Light-Emitting Diodes.

Science.gov (United States)

Hirai, Hiroki; Nakajima, Kiichi; Nakatsuka, Soichiro; Shiren, Kazushi; Ni, Jingping; Nomura, Shintaro; Ikuta, Toshiaki; Hatakeyama, Takuji

2015-11-09

The development of a one-step borylation of 1,3-diaryloxybenzenes, yielding novel boron-containing polycyclic aromatic compounds, is reported. The resulting boron-containing compounds possess high singlet-triplet excitation energies as a result of localized frontier molecular orbitals induced by boron and oxygen. Using these compounds as a host material, we successfully prepared phosphorescent organic light-emitting diodes exhibiting high efficiency and adequate lifetimes. Moreover, using the present one-step borylation, we succeeded in the synthesis of an efficient, thermally activated delayed fluorescence emitter and boron-fused benzo[6]helicene. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

[Evaluation of the effect of one-step self etching adhesives applied in pit and fissure sealing].

Science.gov (United States)

Su, Hong-Ru; Xu, Pei-Cheng; Qian, Wen-Hao

2016-06-01

To observe the effect of three one-step self etching adhesive systems used in fit and fissure sealant and explore the feasibility of application in caries prevention in school. Seven hundred and twenty completely erupted mandibular first molars in 360 children aged 7 to 9 years old were chosen. The split-mouth design was used to select one side as the experimental group, divided into A1(Easy One Adper), B1(Adper Easy One), and C1(iBond SE).The contra lateral teeth served as A2,B2 and C2 groups (phosphoric acid). The retention and caries status were regularly reviewed .The clinical effect of the two groups was compared using SPSS19.0 software package for Chi - square test. At 3 and 6 months, pit and fissure sealant retention rate in A1 and A2, B1 and B2,C1 and C2 group had no significant difference. At 12 months, sealant retention in A1 and B1 group was significantly lower than A2 and B2 group (P0.05). At 24 months, sealant retention rate in A1, B1 and C1 group was significantly lower than A2, B2 and C2 group (P0.05). The clinical anticariogenic effect of three kinds of one-step etching adhesives and phosphoric acid etching sealant was similar .One-step self etching adhesive system was recommended for pit and fissure sealant to improve the students' oral health. The long-term retention rate of one-step self etching adhesive system was lower than the phosphoric acid method to long term observation is needed.

Sensing cocaine in saliva with attenuated total reflection infrared (ATR-IR) spectroscopy combined with a one-step extraction method

Science.gov (United States)

Hans, Kerstin M.-C.; Gianella, Michele; Sigrist, Markus W.

2012-03-01

On-site drug tests have gained importance, e.g., for protecting the society from impaired drivers. Since today's drug tests are majorly only positive/negative, there is a great need for a reliable, portable and preferentially quantitative drug test. In the project IrSens we aim to bridge this gap with the development of an optical sensor platform based on infrared spectroscopy and focus on cocaine detection in saliva. We combine a one-step extraction method, a sample drying technique and infrared attenuated total reflection (ATR) spectroscopy. As a first step we have developed an extraction technique that allows us to extract cocaine from saliva to an almost infrared-transparent solvent and to record ATR spectra with a commercially available Fourier Transform-infrared spectrometer. To the best of our knowledge this is the first time that such a simple and easy-to-use one-step extraction method is used to transfer cocaine from saliva into an organic solvent and detect it quantitatively. With this new method we are able to reach a current limit of detection around 10 μg/ml. This new extraction method could also be applied to waste water monitoring and controlling caffeine content in beverages.

Eight-year randomized clinical evaluation of Class II nanohybrid resin composite restorations bonded with a one-step self-etch or a two-step etch-and-rinse adhesive

DEFF Research Database (Denmark)

van Dijken, Jan WV; Pallesen, Ulla

2015-01-01

(13.5 %) and nine in the two-step etch-and-rinse group (13.0 %). This resulted in nonsignificant different annual failure rates of 1.69 and 1.63 %, respectively. Fracture of restoration was the main reason for failure. Conclusion: Good clinical performance was shown during the 8-year evaluation....... Results: One hundred and fifty-eight restorations were evaluated after 8 years. Three participants with five restorations (three Xeno III, two Excite) were registered as dropouts. Twenty-one failed restorations (13.3 %) were observed during the follow-up. Twelve in the one-step self-etch adhesive group...... and no significant difference in overall clinical performance between the two adhesives. Fracture was the main reason for failure. Clinical relevance: The one-step self-etch adhesive showed a good long-term clinical effectiveness in combination with the nanohybrid resin composite in Class II restorations....

Development process and data management of TurnSTEP, a STEP-compliant CNC system for turning

NARCIS (Netherlands)

Choi, I.; Suh, S.-H; Kim, K.; Song, M.S.; Jang, M.; Lee, B.-E.

2006-01-01

TurnSTEP is one of the earliest STEP-compliant CNC systems for turning. Based on the STEP-NC data model formalized as ISO 14649-12 and 121, it is designed to support intelligent and autonomous control of NC machines for e-manufacturing. The present paper introduces the development process and data

Influence of step complexity and presentation style on step performance of computerized emergency operating procedures

Energy Technology Data Exchange (ETDEWEB)

Xu Song [Department of Industrial Engineering, Tsinghua University, Beijing 100084 (China); Li Zhizhong [Department of Industrial Engineering, Tsinghua University, Beijing 100084 (China)], E-mail: zzli@tsinghua.edu.cn; Song Fei; Luo Wei; Zhao Qianyi; Salvendy, Gavriel [Department of Industrial Engineering, Tsinghua University, Beijing 100084 (China)

2009-02-15

With the development of information technology, computerized emergency operating procedures (EOPs) are taking the place of paper-based ones. However, ergonomics issues of computerized EOPs have not been studied adequately since the industrial practice is quite limited yet. This study examined the influence of step complexity and presentation style of EOPs on step performance. A simulated computerized EOP system was developed in two presentation styles: Style A: one- and two-dimensional flowcharts combination; Style B: two-dimensional flowchart and success logic tree combination. Step complexity was quantified by a complexity measure model based on an entropy concept. Forty subjects participated in the experiment of EOP execution using the simulated system. The results of data analysis on the experiment data indicate that step complexity and presentation style could significantly influence step performance (both step error rate and operation time). Regression models were also developed. The regression analysis results imply that operation time of a step could be well predicted by step complexity while step error rate could only partly predicted by it. The result of a questionnaire investigation implies that step error rate was influenced not only by the operation task itself but also by other human factors. These findings may be useful for the design and assessment of computerized EOPs.

Data-driven Green's function retrieval and application to imaging with multidimensional deconvolution

Science.gov (United States)

Broggini, Filippo; Wapenaar, Kees; van der Neut, Joost; Snieder, Roel

2014-01-01

An iterative method is presented that allows one to retrieve the Green's function originating from a virtual source located inside a medium using reflection data measured only at the acquisition surface. In addition to the reflection response, an estimate of the travel times corresponding to the direct arrivals is required. However, no detailed information about the heterogeneities in the medium is needed. The iterative scheme generalizes the Marchenko equation for inverse scattering to the seismic reflection problem. To give insight in the mechanism of the iterative method, its steps for a simple layered medium are analyzed using physical arguments based on the stationary phase method. The retrieved Green's wavefield is shown to correctly contain the multiples due to the inhomogeneities present in the medium. Additionally, a variant of the iterative scheme enables decomposition of the retrieved wavefield into its downgoing and upgoing components. These wavefields then enable creation of a ghost-free image of the medium with either cross correlation or multidimensional deconvolution, presenting an advantage over standard prestack migration.

Flow-based market coupling. Stepping stone towards nodal pricing?

International Nuclear Information System (INIS)

Van der Welle, A.J.

2012-07-01

For achieving one internal energy market for electricity by 2014, market coupling is deployed to integrate national markets into regional markets and ultimately one European electricity market. The extent to which markets can be coupled depends on the available transmission capacities between countries. Since interconnections are congested from time to time, congestion management methods are deployed to divide the scarce available transmission capacities over market participants. For further optimization of the use of available transmission capacities while maintaining current security of supply levels, flow-based market coupling (FBMC) will be implemented in the CWE region by 2013. Although this is an important step forward, important hurdles for efficient congestion management remain. Hence, flow based market coupling is compared to nodal pricing, which is often considered as the most optimal solution from theoretical perspective. In the context of decarbonised power systems it is concluded that advantages of nodal pricing are likely to exceed its disadvantages, warranting further development of FBMC in the direction of nodal pricing.

Microleakage assessment of one- and two-step self-etch adhesive systems with ‎the low shrinkage composites

Directory of Open Access Journals (Sweden)

Razieh Hoseinifar

2015-07-01

Full Text Available BACKGROUND AND AIM: Different studies evaluating one-step self-etch (SE adhesive systems show contradictory findings, so the aim of this study was to compare the microleakage of one-step SE adhesive systems and CLEARFIL SE BOND (that serves as the “gold-standard” SE adhesive with low shrinkage composites. METHODS: In this in vitro study, Class V cavities with the occlusal margin in enamel and cervical margin in cementum were prepared on the buccal and lingual surfaces of 36 human premolars and molars (72 cavities. The enamel surfaces of the cavities were etched with 37% phosphoric acid and then the specimens were divided into six groups of 6 (12 cavities and the cavities were restored according bellow: Group 1 (Kalore-GC + G-Bond, Group 2 (Grandio + Futurabond NR, Group 3 (Aelite LS Posterior + All Bond SE, Group 4 (Kalore-GC + CLEARFIL SE BOND, Group 5 (Grandio + CLEARFIL SE BOND, and Group 6 (Aelite LS Posterior + CLEARFIL SE BOND. All the specimens were thermocycled for 2000 cycles (5-55 °C and then placed in 0.5% basic fuchsine dye for 24 hours at 37 °C and finally sectioned and observed under the stereomicroscope. Data were analyzed using Kruskal-Wallis, Mann-Whitney, and Wilcoxon tests at a P 0.050. There were no significant differences in microleakage among two-step and one-step SE adhesive systems on both the occlusal and gingival margins. CONCLUSION: According to this study, two-step SE adhesive system (CLEARFIL SE BOND did not provide better marginal seal than the one-step SE adhesive systems.

One-step generation of continuous-variable quadripartite cluster states in a circuit QED system

Science.gov (United States)

Yang, Zhi-peng; Li, Zhen; Ma, Sheng-li; Li, Fu-li

2017-07-01

We propose a dissipative scheme for one-step generation of continuous-variable quadripartite cluster states in a circuit QED setup consisting of four superconducting coplanar waveguide resonators and a gap-tunable superconducting flux qubit. With external driving fields to adjust the desired qubit-resonator and resonator-resonator interactions, we show that continuous-variable quadripartite cluster states of the four resonators can be generated with the assistance of energy relaxation of the qubit. By comparison with the previous proposals, the distinct advantage of our scheme is that only one step of quantum operation is needed to realize the quantum state engineering. This makes our scheme simpler and more feasible in experiment. Our result may have useful application for implementing quantum computation in solid-state circuit QED systems.

Efficient one-step direct labelling of recombinant antibodies with technetium-99m

International Nuclear Information System (INIS)

Liberatore, M.; Neri, D.; Neri, G.; Pini, A.; Lurilli, A.P.; Ponzo, F.; Spampinato, G.; Padula, F.; Pala, A.; Colella, A.C.

1995-01-01

High-affinity bacterially expressed antibody fragments can nowadays be cloned from established hybridomas or, more conveniently, isolated directly from antibody libraries displayed on filamentous phage. Such antibodies can be tagged with C-terminal peptide tags containing one cysteine residue, which represents a convenient functionalisation site for a number of applications, including technetium-99m labelling. Here we describe a simple one-step method for 99m Tc labelling of cysteine-tagged recombinant antibodies with more than 50% radionuclide incorporation. The labelled antibodies displayed full retention of immuoreactivity and good stability. (orig.)

Efficient one-step direct labelling of recombinant antibodies with technetium-99m

Energy Technology Data Exchange (ETDEWEB)

Liberatore, M. [Dipartimento di Medicina Sperimentale, Sezione di Medicina Nucleare, Policlinico Umberto I, Universita di Roma `La Sapienza` (Italy); Neri, D. [Cambridge Centre for Protein Engineering - MRC Centre (United Kingdom); Neri, G. [Dipartimento di Biologia Molecolare, Universita di Siena (Italy); Pini, A. [Dipartimento di Biologia Molecolare, Universita di Siena (Italy); Lurilli, A.P. [Dipartimento di Medicina Sperimentale, Sezione di Medicina Nucleare, Policlinico Umberto I, Universita di Roma `La Sapienza` (Italy); Ponzo, F. [Dipartimento di Medicina Sperimentale, Sezione di Medicina Nucleare, Policlinico Umberto I, Universita di Roma `La Sapienza` (Italy); Spampinato, G. [Laboratorio di Biochimica degli Ormoni Sessuali, Il Instituto di Clinica Ostetrica e Ginecologica, Universita di Roma `La Sapienza` (Italy); Padula, F. [Laboratorio di Biochimica degli Ormoni Sessuali, Il Instituto di Clinica Ostetrica e Ginecologica, Universita di Roma `La Sapienza` (Italy); Pala, A. [Laboratorio di Biochimica degli Ormoni Sessuali, Il Instituto di Clinica Ostetrica e Ginecologica, Universita di Roma `La Sapienza` (Italy); Colella, A.C. [Dipartimento di Medicina Sperimentale, Sezione di Medicina Nucleare, Policlinico Umberto I, Universita di Roma `La Sapienza` (Italy)

1995-11-01

High-affinity bacterially expressed antibody fragments can nowadays be cloned from established hybridomas or, more conveniently, isolated directly from antibody libraries displayed on filamentous phage. Such antibodies can be tagged with C-terminal peptide tags containing one cysteine residue, which represents a convenient functionalisation site for a number of applications, including technetium-99m labelling. Here we describe a simple one-step method for {sup 99m}Tc labelling of cysteine-tagged recombinant antibodies with more than 50% radionuclide incorporation. The labelled antibodies displayed full retention of immuoreactivity and good stability. (orig.)

The prediction of engineering cost for green buildings based on information entropy

Science.gov (United States)

Liang, Guoqiang; Huang, Jinglian

2018-03-01

Green building is the developing trend in the world building industry. Additionally, construction costs are an essential consideration in building constructions. Therefore, it is necessary to investigate the problems of cost prediction in green building. On the basis of analyzing the cost of green building, this paper proposes the forecasting method of actual cost in green building based on information entropy and provides the forecasting working procedure. Using the probability density obtained from statistical data, such as labor costs, material costs, machinery costs, administration costs, profits, risk costs a unit project quotation and etc., situations can be predicted which lead to cost variations between budgeted cost and actual cost in constructions, through estimating the information entropy of budgeted cost and actual cost. The research results of this article have a practical significance in cost control of green building. Additionally, the method proposed in this article can be generalized and applied to a variety of other aspects in building management.

One-pot green synthesis of carbon dots by using Saccharum officinarum juice for fluorescent imaging of bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) cells

Energy Technology Data Exchange (ETDEWEB)

Mehta, Vaibhavkumar N. [Applied Chemistry Department, S. V. National Institute of Technology, Surat, 395 007 (India); Jha, Sanjay [Gujarat Agricultural Biotechnology Institute, Navsari Agricultural University, Surat, 395007 (India); Kailasa, Suresh Kumar, E-mail: sureshkumarchem@gmail.com [Applied Chemistry Department, S. V. National Institute of Technology, Surat, 395 007 (India)

2014-05-01

We are reporting highly economical plant-based hydrothermal method for one-pot green synthesis of water-dispersible fluorescent carbon dots (CDs) by using Saccharum officinarum juice as precursor. The synthesized CDs were characterized by UV-visible, fluorescence, Fourier transform infrared (FT-IR), dynamic light scattering (DLS), high-resolution transmission electron microscopic (HR-TEM), and laser scanning confocal microscopic techniques. The CDs are well dispersed in water with an average size of ∼ 3 nm and showed bright blue fluorescence under UV-light (λ{sub ex} = 365 nm). These CDs acted as excellent fluorescent probes in cellular imaging of bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae). - Highlights: • One-pot green synthesis was used for fluorescent CDs. • FT-IR, DLS, and TEM were used for the characterization of CDs. • The CDs are well dispersed in water with an average size of ∼ 3 nm. • The CDs acted as fluorescent probes for imaging of bacteria and yeast cells.

Some exact identities connecting one- and two-particle Green's functions in spin-orbit coupling systems

International Nuclear Information System (INIS)

Yang Huatong

2007-01-01

Some exact identities connecting one- and two-particle Green's functions in the presence of spin-orbit coupling have been derived. These identities are similar to the Ward identity in usual quantum transport theory of electrons. A satisfying approximate calculation of the spin transport in spin-orbit coupling system should also preserve these identities, just as the Ward identities should be remained in the usual electronic transport theory

Multi-step ahead forecasts for electricity prices using NARX: A new approach, a critical analysis of one-step ahead forecasts

International Nuclear Information System (INIS)

Andalib, Arash; Atry, Farid

2009-01-01

The prediction of electricity prices is very important to participants of deregulated markets. Among many properties, a successful prediction tool should be able to capture long-term dependencies in market's historical data. A nonlinear autoregressive model with exogenous inputs (NARX) has proven to enjoy a superior performance to capture such dependencies than other learning machines. However, it is not examined for electricity price forecasting so far. In this paper, we have employed a NARX network for forecasting electricity prices. Our prediction model is then compared with two currently used methods, namely the multivariate adaptive regression splines (MARS) and wavelet neural network. All the models are built on the reconstructed state space of market's historical data, which either improves the results or decreases the complexity of learning algorithms. Here, we also criticize the one-step ahead forecasts for electricity price that may suffer a one-term delay and we explain why the mean square error criterion does not guarantee a functional prediction result in this case. To tackle the problem, we pursue multi-step ahead predictions. Results for the Ontario electricity market are presented

Soil Quality Assessment Is a Necessary First Step for Designing Urban Green Infrastructure.

Science.gov (United States)

Montgomery, James A; Klimas, Christie A; Arcus, Joseph; DeKnock, Christian; Rico, Kathryn; Rodriguez, Yarency; Vollrath, Katherine; Webb, Ellen; Williams, Allison

2016-01-01

This paper describes the results of a preliminary project conducted by a team of DePaul University undergraduate students and staff from the Gary Comer Youth Center located on Chicago's South Side. The team assessed soil quality on 116 samples collected among four abandoned residential lots adjacent to the Comer Center. Soil quality data will be used in a follow-up study to determine the suitability of each lot for green infrastructure implementation. Green infrastructure may be a useful approach for providing ecosystem services and mitigating food deserts in inner-city communities. Soil quality on all lots was poor. All soils had pH >8.0, low biological activity, and low N mineralization potential. The soils were rich in available P and had mean total Pb concentrations above the USEPA threshold (400 mg kg) for children's playlots. Mean bioavailable Pb on the largest of the four lots was 12% of total Pb, indicating that most of the total Pb is not bioavailable. This result is encouraging because high bioavailable Pb concentrations are linked with negative health effects, particularly in children. All lots had NO-N concentrations below those considered to be appropriate for plant growth. On the other hand, no significant differences in mean concentrations of the other analytes were found. The poor soil quality in the four lots presents an opportunity to use green infrastructure to enhance ecosystem services, improve community and environmental health, and provide more equitable access to green space. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.

Saccharide-based Approach to Green Metallic Nanostructure Synthesis

DEFF Research Database (Denmark)

Engelbrekt, Christian; Sørensen, Karsten Holm; Jensen, Palle Skovhus

A green approach to solution synthesis of metallic nanoparticles has been developed using harmless and bioapplicable chemicals as well as moderate temperatures. Metal precursors are reduced by glucose/buffers and sterically stabilized by starch. The saccharide based procedure is highly diverse pr...... producing specifically a wide range of spherical, anisotropic, metallic, semi - conductor and core-shell nanostructures....

Constructing an exposure chart: step by step (based on standard procedures)

International Nuclear Information System (INIS)

David, Jocelyn L; Cansino, Percedita T.; Taguibao, Angileo P.

2000-01-01

An exposure chart is very important in conducting radiographic inspection of materials. By using an accurate exposure chart, an inspector is able to avoid a trial and error way of determining correct time to expose a specimen, thereby producing a radiograph that has an acceptable density based on a standard. The chart gives the following information: x-ray machine model and brand, distance of the x-ray tube from the film, type and thickness of intensifying screens, film type, radiograph density, and film processing conditions. The methods of preparing an exposure chart are available in existing radiographic testing manuals. These described methods are presented in step by step procedures, covering the actual laboratory set-up, data gathering, computations, and transformation of derived data into Characteristic Curve and Exposure Chart

Urban green spaces' effectiveness as a psychological buffer for the negative health impact of noise pollution: a systematic review.

Science.gov (United States)

Dzhambov, Angel Mario; Dimitrova, Donka Dimitrova

2014-01-01

Noise pollution is one of the four major pollutions in the world. Little evidence exists about the actual preventive benefits of psychological noise attenuation by urban green spaces, especially from the perspective of environmental medicine and, to the best of our knowledge, there is not a systematic analysis on this topic. The aim of this review was to systematically evaluate whether there is conclusive scientific evidence for the effectiveness of urban green spaces as a psychological buffer for the negative impact of noise pollution on human health and to promote an evidence-based approach toward this still growing environmental hazard. MEDLINE and EMBASE databases were searched for experimental and epidemiological studies published before June 04, 2013 in English and Spanish. Data was independently extracted in two step process by the authors. Due to the heterogeneity of the included studies qualitative assessment was performed. We found moderate evidence that the presence of vegetation can generally reduce the negative perception of noise (supported with an electroencephalogram test in one of the experimental studies; consistent with the data from two epidemiological studies; one experiment found no effect and one was inconclusive about the positive effect). This review fills a gap in the literature and could help researchers further clarify the proper implementation of urban green spaces as a psychological buffer in areas with population exposed to chronic noise pollution.

A Computer Clone of Human Expert for Mobility Management Scheme (E-MMS): Step toward Green Transportation

Science.gov (United States)

Resdiansyah; O. K Rahmat, R. A.; Ismail, A.

2018-03-01

Green transportation refers to a sustainable transport that gives the least impact in terms of social and environmental but at the same time is able to supply energy sources globally that includes non-motorized transport strategies deployment to promote healthy lifestyles, also known as Mobility Management Scheme (MMS). As construction of road infrastructure cannot help solve the problem of congestion, past research has shown that MMS is an effective measure to mitigate congestion and to achieve green transportation. MMS consists of different strategies and policies that subdivided into categories according to how they are able to influence travel behaviour. Appropriate selection of mobility strategies will ensure its effectiveness in mitigating congestion problems. Nevertheless, determining appropriate strategies requires human expert and depends on a number of success factors. This research has successfully developed a computer clone system based on human expert, called E-MMS. The process of knowledge acquisition for MMS strategies and the next following process to selection of strategy has been encode in a knowledge-based system using a shell expert system. The newly developed computer cloning system was successfully verified, validated and evaluated (VV&E) by comparing the result output with the real transportation expert recommendation in which the findings suggested Introduction

Multi-step wind speed forecasting based on a hybrid forecasting architecture and an improved bat algorithm

International Nuclear Information System (INIS)

Xiao, Liye; Qian, Feng; Shao, Wei

2017-01-01

Highlights: • Propose a hybrid architecture based on a modified bat algorithm for multi-step wind speed forecasting. • Improve the accuracy of multi-step wind speed forecasting. • Modify bat algorithm with CG to improve optimized performance. - Abstract: As one of the most promising sustainable energy sources, wind energy plays an important role in energy development because of its cleanliness without causing pollution. Generally, wind speed forecasting, which has an essential influence on wind power systems, is regarded as a challenging task. Analyses based on single-step wind speed forecasting have been widely used, but their results are insufficient in ensuring the reliability and controllability of wind power systems. In this paper, a new forecasting architecture based on decomposing algorithms and modified neural networks is successfully developed for multi-step wind speed forecasting. Four different hybrid models are contained in this architecture, and to further improve the forecasting performance, a modified bat algorithm (BA) with the conjugate gradient (CG) method is developed to optimize the initial weights between layers and thresholds of the hidden layer of neural networks. To investigate the forecasting abilities of the four models, the wind speed data collected from four different wind power stations in Penglai, China, were used as a case study. The numerical experiments showed that the hybrid model including the singular spectrum analysis and general regression neural network with CG-BA (SSA-CG-BA-GRNN) achieved the most accurate forecasting results in one-step to three-step wind speed forecasting.

Green Buildings Amid Efficiency and Sustainability

International Nuclear Information System (INIS)

Khalifa, Yousef M.; Mady, Salah M.

2014-01-01

The vast increase of energy consumption, global warming and the harm they cause to the environment, emerge to be a major obstruction that distresses the world today. This review paper views one of the methods that the world focused on as means of reducing the environmental harms and that is, through green building, or in more common words sustainable buildings. Those means, covers the exercising of a wide range of applications including merging of new and specific technologies in which through fulfilling its basis, the process of evaluation of the building takes place in terms of its harmony with the environment, reduction of energy consumption, and the reduction of the environmental problems caused by the building life cycle starting from defining of location, design of the building, operation, maintenance, repairing and up to the renewal of the building. Despite the significance of green building, no profit nor implementations has yet been made in Libya. The latter is due to the lack of awareness by many Libyan social groups. From here, the idea behind this paper crystallized. It aims to spread and enhance the knowledge and techniques of green building. It also penetrates into the green building features and advantages that are considered to be a preliminary step to start its application in a wide range coinciding with the grand progress that the country has witnessed in the field of construction and housing. This paper concludes that it is possible to reduce energy consumption and the harm it causes to the environment after the implementation and merging of green building techniques and should be applied on a large scale covering the whole country.(author)

Economic versus belief-based models: Shedding light on the adoption of novel green technologies

International Nuclear Information System (INIS)

Girod, Bastien; Mayer, Sebastian; Nägele, Florian

2017-01-01

Understanding the determinants for the adoption of novel green consumer technologies is important to effectively foster their diffusion. Energy and environmental science literature often takes an approach based on economic variables such as objectively measureable household and technology characteristics. Increasingly, also subjective variables based on personal belief are considered. On the basis of a survey about the intention to adopt an exemplary novel green consumer technology (intelligent thermostats), we contribute to the clarification of the explanatory power of these two approaches. We first compare the economic model to the belief-based model and second, investigate how beliefs about the green technology are influenced by personal environmental norms and innovativeness. Our evaluation shows that the belief-based model explains considerably higher variance in the intention to adopt. Thereby the perceived hedonic satisfaction, usefulness, habit and facilitating conditions reveal as key determinants. Moreover, environmental norms show lower impact than personal innovativeness. In the discussion we consolidate these findings and point to the risk of omitted variable bias when selectively including belief-based variables in adoption models. Our findings suggest that policies can effectively accelerate the early market diffusion of green consumer technologies by incentivizing retailers to introduce and market such technologies. - Highlights: • Adoption of a green consumer technology (energy-saving thermostats) is evaluated. • Subjective beliefs about the technology show higher impact than objective measures. • Key beliefs relate to pleasure, usefulness, habits and facilitating conditions. • Personal innovativeness is more relevant for adoption than environmental norms. • Isolated use of belief-based adoption determinants can lead to omitted variable bias.

Downstream-based Scheduling for Energy Conservation in Green EPONs

KAUST Repository

Chen, Shen; Dhaini, Ahmad R.; Ho, Pin-Han; Shihada, Basem; Shen, Gangxiang; Lin, Chih-Hao

2012-01-01

the ONU sleep time, it jeopardizes the quality of service (QoS) performance of the network, especially for downstream traffic in case the overlapping is based on the upstream time slot. In this paper, we study the downstream traffic performance in green

Molecular detection of Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola, and Burkholderia glumae in infected rice seeds and leaves

Science.gov (United States)

Polymerase chain reaction (PCR) is particularly useful for plant pathogen detection. In the present study, multiplex PCR and SYBR green real-time PCR were developed to facilitate simultaneous detection of three important rice pathogens, Xanthomonas oryzae pv. oryzae, X. oryzae pv. oryzicola, and Bur...

A G-quadruplex-based Label-free Fluorometric Aptasensor for Adenosine Triphosphate Detection.

Science.gov (United States)

Li, Li Juan; Tian, Xue; Kong, Xiang Juan; Chu, Xia

2015-01-01

A G-quadruplex-based, label-free fluorescence assay was demonstrated for the detection of adenosine triphosphate (ATP). A double-stranded DNA (dsDNA), hybridized by ATP-aptamer and its complementary sequence, was employed as a substrate for ATP binding. SYBR Green I (SG I) was a fluorescent probe and exonuclease III (Exo III) was a nuclease to digest the dsDNA. Consequently, in the absence of ATP, the dsDNA was inset with SG I and was digested by Exo III, resulting in a low background signal. In the presence of ATP, the aptamer in dsDNA folded into a G-quadruplex structure that resisted the digestion of Exo III. SG I was inserted into the structure, showing high fluorescence. Owing to a decrease of the background noise, a high signal-to-noise ratio could be obtained. This sensor can detect ATP with a concentration ranging from 50 μM to 5 mM, and possesses a capacity for the sensitive determination of other targets.

One-step inkjet printing of tungsten oxide-poly(3,4-ethylenedioxythiophene):polystyrene sulphonate hybrid film and its applications in electrochromic devices

Energy Technology Data Exchange (ETDEWEB)

Nguyen, Thi-Thuy-Nga, E-mail: thuysnga@gmail.com; Chan, Chih-Yu; He, Ju-Liang

2016-03-31

Hybrid film comprised tungsten oxide and poly (3,4-ethylenedioxythiophene):polystyrene sulphonate (WO{sub 3}–PEDOT:PSS) was developed by applying one-step inkjet printing from an office inkjet printer. The WO{sub 3} nanoparticles were synthesized from commercial crystalline WO{sub 3} powder through a wet ball-milling process, which is a simple, environmentally friendly, and cost-effective method of using water as a green solvent and low-energy milling. The WO{sub 3}–PEDOT:PSS inkjet ink was prepared by dispersing the as-milled WO{sub 3} and PEDOT:PSS in n-propanol and deionized water. The inkjet-printed WO{sub 3}–PEDOT:PSS thin films show marked improvements of cathodic electrochromism over WO{sub 3} films: the transmittance change of 20% at 550 nm (visible region) and 35% at 900 nm (infrared region) along with the response time of 5.67/0.30 s in their colored/bleached state, and the electrochromic coloration efficiency of 27.86 cm{sup 2}/C at 550 nm and 69.64 cm{sup 2}/C at 900 nm. - Highlights: • WO{sub 3} nanoparticles were synthesized by milling commercial crystalline WO{sub 3}. • Wet ball-milling was carried out by using water as a green solvent and low energy. • WO{sub 3}–PEDOT:PSS hybrid ink was simply prepared by adding n-propanol and DI water. • WO{sub 3}–PEDOT:PSS hybrid films were inkjet-printed via an office inkjet printer. • WO{sub 3}–PEDOT:PSS films show better electrochromic performances than WO{sub 3} films.

Generic and sequence-variant specific molecular assays for the detection of the highly variable Grapevine leafroll-associated virus 3.

Science.gov (United States)

Chooi, Kar Mun; Cohen, Daniel; Pearson, Michael N

2013-04-01

Grapevine leafroll-associated virus 3 (GLRaV-3) is an economically important virus, which is found in all grapevine growing regions worldwide. Its accurate detection in nursery and field samples is of high importance for certification schemes and disease management programmes. To reduce false negatives that can be caused by sequence variability, a new universal primer pair was designed against a divergent sequence data set, targeting the open reading frame 4 (heat shock protein 70 homologue gene), and optimised for conventional one-step RT-PCR and one-step SYBR Green real-time RT-PCR assays. In addition, primer pairs for the simultaneous detection of specific GLRaV-3 variants from groups 1, 2, 6 (specifically NZ-1) and the outlier NZ2 variant, and the generic detection of variants from groups 1 to 5 were designed and optimised as a conventional one-step multiplex RT-PCR assay using the plant nad5 gene as an internal control (i.e. one-step hexaplex RT-PCR). Results showed that the generic and variant specific assays detected in vitro RNA transcripts from a range of 1×10(1)-1×10(8) copies of amplicon per μl diluted in healthy total RNA from Vitis vinifera cv. Cabernet Sauvignon. Furthermore, the assays were employed effectively to screen 157 germplasm and 159 commercial field samples. Thus results demonstrate that the GLRaV-3 generic and variant-specific assays are prospective tools that will be beneficial for certification schemes and disease management programmes, as well as biological and epidemiological studies of the divergent GLRaV-3 populations. Copyright © 2013 Elsevier B.V. All rights reserved.

Hydrogen with intrinsic CO{sub 2} sequestration: the ENI 'One Step Hydrogen' process

Energy Technology Data Exchange (ETDEWEB)

Rossini, S.; Cornaro, U.; Mizia, F.; Malandrino, A.; Piccoli, V. [Enitecnologie SpA, S. Donata Milanese (Italy); Sanfilippo, D.; Miracca, I. [Snamprogetti SpA, S. Donato Milanese (Italy)

2003-07-01

The new process, under development in our companies, is aimed to hydrogen production with intrinsic carbon dioxide sequestration. This is made possible combining in a cycle the water oxidative potential with a reverse action by a reducing agent like hydrocarbons, the preferred being natural gas. In the first step a suitable oxide takes up the oxygen from water splitting producing hydrogen. The solid act as an oxygen storage medium. Such 'lattice' oxygen is in turn released through one or more elemental steps. The process fits very well with a circulating fluid bed reactor which allows the movement of the solid from one reactive environment to the other. (orig.)

Effect of alternative modes of application on microleakage of one-step self-etch adhesives

Directory of Open Access Journals (Sweden)

Mustafa Ulker

2013-12-01

Conclusion: Alternative modes of application did not adversely affect the sealing ability of one-step self-etch adhesives. Depending on the type of adhesive, alternative modes of application may contribute to low microleakage values.

Target-induced structure switching of hairpin aptamers for label-free and sensitive fluorescent detection of ATP via exonuclease-catalyzed target recycling amplification.

Science.gov (United States)

Xu, Yunying; Xu, Jin; Xiang, Yun; Yuan, Ruo; Chai, Yaqin

2014-01-15

In this work, we described the development of a new label-free, simple and sensitive fluorescent ATP sensing platform based on exonuclease III (Exo III)-catalyzed target recycling (ECTR) amplification and SYBR Green I indicator. The hairpin aptamer probes underwent conformational structure switching and re-configuration in the presence of ATP, which led to catalytic cleavage of the re-configured aptamers by Exo III to release ATP and to initiate the ECTR process. Such ECTR process resulted in the digestion of a significant number of the hairpin aptamer probes, leading to much less intercalation of SYBR Green I to the hairpin stems and drastic suppression of the fluorescence emission for sensitive ATP detection down to the low nanomolar level. Due to the highly specific affinity bindings between aptamers and ATP, the developed method exhibited excellent selectivity toward ATP against other analogous molecules. Besides, our ATP sensing approach used un-modified aptamer probes and could be performed in a "mix-and-detect" fashion in homogenous solutions. All these distinct advantages of the developed method thus made it hold great potential for the development of simple and robust sensing strategies for the detection of other small molecules. © 2013 Elsevier B.V. All rights reserved.

Endpoint visual detection of three genetically modified rice events by loop-mediated isothermal amplification.

Science.gov (United States)

Chen, Xiaoyun; Wang, Xiaofu; Jin, Nuo; Zhou, Yu; Huang, Sainan; Miao, Qingmei; Zhu, Qing; Xu, Junfeng

2012-11-07

Genetically modified (GM) rice KMD1, TT51-1, and KF6 are three of the most well known transgenic Bt rice lines in China. A rapid and sensitive molecular assay for risk assessment of GM rice is needed. Polymerase chain reaction (PCR), currently the most common method for detecting genetically modified organisms, requires temperature cycling and relatively complex procedures. Here we developed a visual and rapid loop-mediated isothermal amplification (LAMP) method to amplify three GM rice event-specific junction sequences. Target DNA was amplified and visualized by two indicators (SYBR green or hydroxy naphthol blue [HNB]) within 60 min at an isothermal temperature of 63 °C. Different kinds of plants were selected to ensure the specificity of detection and the results of the non-target samples were negative, indicating that the primer sets for the three GM rice varieties had good levels of specificity. The sensitivity of LAMP, with detection limits at low concentration levels (0.01%−0.005% GM), was 10- to 100-fold greater than that of conventional PCR. Additionally, the LAMP assay coupled with an indicator (SYBR green or HNB) facilitated analysis. These findings revealed that the rapid detection method was suitable as a simple field-based test to determine the status of GM crops.

Endpoint Visual Detection of Three Genetically Modified Rice Events by Loop-Mediated Isothermal Amplification

Directory of Open Access Journals (Sweden)

Qing Zhu

2012-11-01

Full Text Available Genetically modified (GM rice KMD1, TT51-1, and KF6 are three of the most well known transgenic Bt rice lines in China. A rapid and sensitive molecular assay for risk assessment of GM rice is needed. Polymerase chain reaction (PCR, currently the most common method for detecting genetically modified organisms, requires temperature cycling and relatively complex procedures. Here we developed a visual and rapid loop-mediated isothermal amplification (LAMP method to amplify three GM rice event-specific junction sequences. Target DNA was amplified and visualized by two indicators (SYBR green or hydroxy naphthol blue [HNB] within 60 min at an isothermal temperature of 63 °C. Different kinds of plants were selected to ensure the specificity of detection and the results of the non-target samples were negative, indicating that the primer sets for the three GM rice varieties had good levels of specificity. The sensitivity of LAMP, with detection limits at low concentration levels (0.01%–0.005% GM, was 10- to 100-fold greater than that of conventional PCR. Additionally, the LAMP assay coupled with an indicator (SYBR green or HNB facilitated analysis. These findings revealed that the rapid detection method was suitable as a simple field-based test to determine the status of GM crops.

Compatibility of pedigree-based and marker-based relationship matrices for single-step genetic evaluation

DEFF Research Database (Denmark)

Christensen, Ole Fredslund

2012-01-01

Single-step methods for genomic prediction have recently become popular because they are conceptually simple and in practice such a method can completely replace a pedigree-based method for routine genetic evaluation. An issue with single-step methods is compatibility between the marker-based rel...

Research on test of product based on spatial sampling criteria and variable step sampling mechanism

Science.gov (United States)

Li, Ruihong; Han, Yueping

2014-09-01

This paper presents an effective approach for online testing the assembly structures inside products using multiple views technique and X-ray digital radiography system based on spatial sampling criteria and variable step sampling mechanism. Although there are some objects inside one product to be tested, there must be a maximal rotary step for an object within which the least structural size to be tested is predictable. In offline learning process, Rotating the object by the step and imaging it and so on until a complete cycle is completed, an image sequence is obtained that includes the full structural information for recognition. The maximal rotary step is restricted by the least structural size and the inherent resolution of the imaging system. During online inspection process, the program firstly finds the optimum solutions to all different target parts in the standard sequence, i.e., finds their exact angles in one cycle. Aiming at the issue of most sizes of other targets in product are larger than that of the least structure, the paper adopts variable step-size sampling mechanism to rotate the product specific angles with different steps according to different objects inside the product and match. Experimental results show that the variable step-size method can greatly save time compared with the traditional fixed-step inspection method while the recognition accuracy is guaranteed.

Growing your green chemistry mindset

Science.gov (United States)

Kosmas, Steven

2017-08-01

The purpose of this article is not to delineate the steps to move across the continuum to being a greener chemist, but to analyse the cognitive processes involved in fostering a green chemistry growth mindset (GCGM) [Dweck C. (2006) Mindset: The New Psychology of Success. New York, NY: Ballatine]. The focus is on changing the mindset, which inevitably will lead to a more mindful approach to chemistry practices before the laboratory begins. A green chemistry fixed mindset (GCFM) is closed to making improvements, since the attitude is that the techniques and processes in the laboratory are already employing a green chemistry mindset [Dweck C. (2006) Mindset: The New Psychology of Success. New York, NY: Ballatine]. The problem with the GCFM is that it precludes the possibility of making improvements. However, the GCGM employs a continuous, intentional focus on the attitude towards green chemistry, with the ultimate goal being a change in chemistry practices that is greener. The focus of this article will be on the GCGM.

Designing Green Networks and Network Operations Saving Run-the-Engine Costs

CERN Document Server

Minoli, Daniel

2011-01-01

In recent years the confluence of socio-political trends toward environmental responsibility and the pressing need to reduce Run-the-Engine (RTE) costs has given birth to a nascent discipline of Green IT. A clear and concise introduction to green networks and green network operations, this book examines analytical measures and discusses virtualization, network computing, and web services as approaches for green data centers and networks. It identifies some strategies for green appliance and end devices and examines the methodical steps that can be taken over time to achieve a seamless migratio

In situ biosynthesis of bacterial nanocellulose-CaCO{sub 3} hybrid bionanocomposite: One-step process

Energy Technology Data Exchange (ETDEWEB)

Mohammadkazemi, Faranak, E-mail: f_mkazemi@sbu.ac.ir [Department of Cellulose and Paper Technology, Faculty of New Technologies Engineering, Shahid Beheshti University, Science and Research Campus, Zirab, Savadkooh, Mazandaran (Iran, Islamic Republic of); Faria, Marisa; Cordeiro, Nereida [Faculty of Exact Science and Engineering, University of Madeira, Funchal (Portugal)

2016-08-01

In this work, a simple and green route to the synthesis of the bacterial nanocellulose-calcium carbonate (BNC/CaCO{sub 3}) hybrid bionanocomposites using one-step in situ biosynthesis was studied. The CaCO{sub 3} was incorporated in the bacterial nanocellulose structure during the cellulose biosynthesis by Gluconacetobacter xylinus PTCC 1734 bacteria. Hestrin-Schramm (HS) and Zhou (Z) culture media were used to the hybrid bionanocomposites production and the effect of ethanol addition was investigated. Attenuated total reflection Fourier transform infrared spectroscopy, field emission scanning electron microscopy, X-ray diffraction, energy-dispersive X-ray spectroscopy, inverse gas chromatography and thermogravimetric analysis were used to characterize the samples. The experimental results demonstrated that the ethanol and culture medium play an important role in the BNC/CaCO{sub 3} hybrid bionanocomposites production, structure and properties. The BNC/CaCO{sub 3} biosynthesized in Z culture medium revealed higher O/C ratio and amphoteric surface character, which justify the highest CaCO{sub 3} content incorporation. The CaCO{sub 3} was incorporated into the cellulosic matrix decreasing the bacterial nanocellulose crystallinity. This work reveals the high potential of in situ biosynthesis of BNC/CaCO{sub 3} hybrid bionanocomposites and opens a new way to the high value-added applications of bacterial nanocellulose. - Graphical Abstract: Display Omitted - Highlights: • BNC/CaCO{sub 3} hybrid bionanocomposites were produced using in situ biosynthesis process. • Ethanol and culture medium play an important role in the production and properties. • Z-BNC/CaCO{sub 3} bionanocomposites revealed higher O/C ratio and amphoteric surface character. • CaCO{sub 3} incorporated into the BNC decreased crystallinity.

Metaphysical green

DEFF Research Database (Denmark)

Earon, Ofri

2011-01-01

example is a tiny Danish summer house from 1918 . The second example is ‘House before House’ , in Tokyo. The third example is a prefabricated house ‘CHU’ . The analysis evaluates the characteristics of diverse tones of green – from green image to green sensation. The analysis is based on the original...... of Sensation of Green is created by a physical interaction between the language of space and the language of nature” The notion of Sensation of Green was developed through a previous study ‘Learning from the Summer House’ investigating the unique architectural characteristics of the Danish summer houses...... the Sensation of Green? Three existing examples are agents to this discussion. The first example is a Danish summer house. The other two are international urban examples. While the summer house articulates the original meaning of Sensation of Green, the urban examples illustrate its urban context. The first...

Green roof and storm water management policies: monitoring experiments on the ENPC Blue Green Wave

Science.gov (United States)

Versini, Pierre-Antoine; Gires, Auguste; Fitton, George; Tchiguirinskaia, Ioulia; Schertzer, Daniel

2015-04-01

Currently widespread in new urban projects, green roofs have shown a positive impact on urban runoff at the building/parcel scale. Nevertheless, there is no specific policy promoting their implementation neither in Europe nor in France. Moreover they are not taken into account (and usually considered as an impervious area) in the sizing of a retention basin for instance. An interesting example is located in the heart of the Paris-East Cluster for Science and Technology (Champs-sur-Marne, France). Since 2013 a large (1 ha) wavy-form vegetated roof (called bleu green wave) is implemented. Green roof area and impervious areas are connected to a large retention basin, which has been oversized. The blue green wave represents a pioneering site where an initially amenity (decorative) design project has been transformed into a research oriented one. Several measurement campaigns have been conducted to investigate and better understand the hydrological behaviour of such a structure. Rainfall, humidity, wind velocity, water content and temperature have been particularly studied. The data collected are used for several purposes: (i) characterize the spatio-temporal variability of the green roof response, (ii) calibrate and validate a specific model simulating its hydrological behavior. Based on monitoring and modeling results, green roof performances will be quantified. It will be possible to estimate how they can reduce stormwater runoff and how these performances can vary in space and in time depending on green roof configuration, rainfall event characteristics and antecedent conditions. These quantified impacts will be related to regulation rules established by stormwater managers in order to connect the parcel to the sewer network. In the particular case of the building of a retention basin, the integration of green roof in the sizing of the basin will be studied. This work is funded by the European Blue Green Dream project (http://bgd.org.uk/, funded by Climate

Design-Based Research in Science Education: One Step Towards Methodology

Directory of Open Access Journals (Sweden)

Kalle Juuti

2012-10-01

Full Text Available Recently, there has been critiques towards science education research, as the potential of this research has not been actualised in science teaching and learning praxis. The paper describes an analysis of a design-based research approach (DBR that has been suggested as a solution for the discontinuation between science education research and praxis. We propose that a pragmatic frame helps to clarify well the design-based research endeavour. We abstracted three aspects from the analysis that constitute design-based research: (a a design process is essentially iterative starting from the recognition of the change of the environment of praxis, (b it generates a widely usable artefact, (c and it provides educational knowledge for more intelligible praxis. In the knowledge acquisition process, the pragmatic viewpoint emphasises the role of a teacher’s reflected actions as well as the researches’ involvement in the authentic teaching and learning settings.

Iteratively improving Hi-C experiments one step at a time.

Science.gov (United States)

Golloshi, Rosela; Sanders, Jacob T; McCord, Rachel Patton

2018-04-30

The 3D organization of eukaryotic chromosomes affects key processes such as gene expression, DNA replication, cell division, and response to DNA damage. The genome-wide chromosome conformation capture (Hi-C) approach can characterize the landscape of 3D genome organization by measuring interaction frequencies between all genomic regions. Hi-C protocol improvements and rapid advances in DNA sequencing power have made Hi-C useful to study diverse biological systems, not only to elucidate the role of 3D genome structure in proper cellular function, but also to characterize genomic rearrangements, assemble new genomes, and consider chromatin interactions as potential biomarkers for diseases. Yet, the Hi-C protocol is still complex and subject to variations at numerous steps that can affect the resulting data. Thus, there is still a need for better understanding and control of factors that contribute to Hi-C experiment success and data quality. Here, we evaluate recently proposed Hi-C protocol modifications as well as often overlooked variables in sample preparation and examine their effects on Hi-C data quality. We examine artifacts that can occur during Hi-C library preparation, including microhomology-based artificial template copying and chimera formation that can add noise to the downstream data. Exploring the mechanisms underlying Hi-C artifacts pinpoints steps that should be further optimized in the future. To improve the utility of Hi-C in characterizing the 3D genome of specialized populations of cells or small samples of primary tissue, we identify steps prone to DNA loss which should be considered to adapt Hi-C to lower cell numbers. Copyright © 2018 Elsevier Inc. All rights reserved.

A step-by-step introduction to vegetables at the beginning of complementary feeding. The effects of early and repeated exposure.

Science.gov (United States)

Hetherington, Marion M; Schwartz, C; Madrelle, J; Croden, F; Nekitsing, C; Vereijken, C M J L; Weenen, H

2015-01-01

Breastfeeding (BF) is associated with willingness to accept vegetables. This may be due to the variety of flavours delivered via breast milk. Some mothers add vegetables to milk during complementary feeding (CF) to enhance acceptance. The present study tested a step-by-step exposure to vegetables in milk then rice during CF, on intake and liking of vegetables. Just before CF, enrolled mothers were randomised to an intervention (IG, n = 18; 6 BF) or control group (CG, n = 18; 6 BF). IG infants received 12 daily exposures to vegetable puree added to milk (days 1-12), then 12 × 2 daily exposures to vegetable puree added to rice at home (days 13-24). Plain milk and rice were given to CG. Then both received 11 daily exposures to vegetable puree. Intake was weighed and liking rated on days 25-26 and 33-35 after the start of CF in the laboratory, supplemented by the same data recorded at home. Vegetables were rotated daily (carrots, green beans, spinach, broccoli). Intake, liking and pace of eating were greater for IG than CG infants. Intake and liking of carrots were greater than green beans. However, at 6m then 18m follow up, vegetable (carrot > green beans) but not group differences were observed. Mothers reported appreciation of the structure and guidance of this systematic approach. Early exposure to vegetables in a step-by-step method could be included in CF guidelines and longer term benefits assessed by extending the exposure period. Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.

Synthesis and characterization of sulfate and dodecylbenzenesulfonate intercalated zinc-iron layered double hydroxides by one-step coprecipitation route

International Nuclear Information System (INIS)

Zhang Hui; Wen Xing; Wang Yingxia

2007-01-01

Inorganic sulfate- and organic dodecylbenzenesulfonate (DBS)-intercalated zinc-iron layered double hydroxides (LDHs) materials were prepared by one-step coprecipitation method from a mixed salt solutions containing Zn(II), Fe(II) and Fe(III) salts. The as-prepared samples have been characterized by X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), low-temperature nitrogen adsorption, scanning electron microscopy (SEM), inductively coupled plasma emission spectroscopy (ICP), and Moessbauer spectroscopy (MS). The XRD analyses demonstrate the typical LDH-like layered structural characteristics of both products. The room temperature MS results reveal the characteristics of both the Fe(II) and Fe(III) species for SO 4 2- -containing product, while only the Fe(III) characteristic for DBS-containing one. The combination characterization results and Rietveld analysis illustrate that the SO 4 2- -containing product possesses the Green Rust two (GR2)-like crystal structure with an approximate chemical composition of [Zn 0.435 .Fe II 0.094 .Fe III 0.470 .(OH) 2 ].(SO 4 2- ) 0.235 .1.0H 2 O, while the DBS-containing one exhibits the common LDH compound-like structure. The contact angle measurement indicates the evident hydrophobic properties of DBS-containing nanocomposite, compared with SO 4 2- -containing product, due to the modification of the internal and external surface of LDHs by the organic hydrophobic chain of DBS. - Graphical abstract: For Zn 2+ -Fe 2+ -Fe 3+ GR2(SO 4 2- ), according to the derived chemical formula, Fe 3+ was arranged at 1a (0, 0, 0) position, while all Zn 2+ were in 2d position with the occupancy 0.645, and the left part of 2d positions were taken by Fe 2+ /Fe 3+

A comprehensive typology for mainstreaming urban green infrastructure

Science.gov (United States)