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Sample records for okra hibiscus esculentus

  1. Okra (Hibiscus esculentus) seed oil for biodiesel production

    Anwar, Farooq; Nadeem, Muhammad [Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad 38040 (Pakistan); Rashid, Umer [Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad 38040 (Pakistan); Department of Industrial Chemistry, Government College University, Faisalabad 38000 (Pakistan); Ashraf, Muhammad [Department of Botany, University of Agriculture, Faisalabad 38040 (Pakistan)

    2010-03-15

    Biodiesel was derived from okra (Hibiscus esculentus) seed oil by methanol-induced transesterification using an alkali catalyst. Transesterification of the tested okra seed oil under optimum conditions: 7:1 methanol to oil molar ratio, 1.00% (w/w) NaOCH{sub 3} catalyst, temperature 65 C and 600 rpm agitation intensity exhibited 96.8% of okra oil methyl esters (OOMEs) yield. The OOMEs/biodiesel produced was analyzed by GC/MS, which showed that it mainly consisted of four fatty acids: linoleic (30.31%), palmitic (30.23%), oleic (29.09%) and stearic (4.93%). A small amount of 2-octyl cyclopropaneoctanoic acid with contribution 1.92% was also established. Fuel properties of OOMEs such as density, kinematic viscosity, cetane number, oxidative stability, lubricity, flash point, cold flow properties, sulfur contents and acid value were comparable with those of ASTM D 6751 and EN 14214, where applicable. It was concluded that okra seed oil is an acceptable feedstock for biodiesel production. (author)

  2. Preparation and Characterization of Polyelectrolyte Complexes of Hibiscus esculentus (Okra Gum and Chitosan

    Vivekjot Brar

    2018-01-01

    Full Text Available Polyelectrolyte complexes (PECs of Okra gum (OKG extracted from fruits of Hibiscus esculentus (Malvaceae and chitosan (CH were prepared using ionic gelation technique. The PECs were insoluble and maximum yield was obtained at weight ratio of 7 : 3. The supernatant obtained after extracting PECs was clearly representing complete conversion of polysaccharides into PECs. Complexation was also evaluated by measuring the viscosity of supernatant after precipitation of PECs. The dried PECs were characterized using FTIR, DSC, zeta potential, water uptake, and SEM studies. Thermal analysis of PECs prepared at all ratios (10 : 90, 20 : 80, 30 : 70, 40 : 60, 50 : 50, 60 : 40, 70 : 30, 80 : 20, and 90 : 10; OKG : CH depicted an endothermic peak at approximately 240°C representing cleavage of electrostatic bond between OKG and CH. The optimized ratio (7 : 3 exhibited a zeta potential of −0.434 mV and displayed a porous structure in SEM analysis. These OKG-CH PECs can be further employed as promising carrier for drug delivery.

  3. SEEDS AND Abelmoschus esculentus (OKRA)

    user

    Department of Chemistry, Federal University of Technology, Minna, Nigeria. 2. Science Laboratory Technology, Federal Polytechnic Bida, Niger State. * email: johntsadom@gmail. ... Abelmoschus esculentus pods was carried out using standard methods. The results of the ..... plants make to food security? Acta. Horticulture.

  4. Regeneration of okra ( Abelmoschus esculentus L.) via apical shoot ...

    Abelmoschus esculentus L. Monech) via apical shoot culture system. The study of apical shoot culture system was found effective for regeneration of apical shoots. The okra (A. esculentus L. Monech) N-550 line evolved at R&D, Nirmal Seeds Pvt. Ltd., ...

  5. Development of Abelmoschus esculentus (Okra)-Based ...

    Results: Okra gel formulation showed good properties in terms of pH, viscosity and mucoadhesion. ... filter and precipitated with three times the volume ... 2 days in hot air oven (NAAY-M40, Naugra, .... biodegradable nature as well as likely low.

  6. Study of the hibiscus esculentus mucilage coagulation–flocculation ...

    The flocculent activity of Hibiscus esculentus (gombo) mucilage traditionally used for a local beer (Tchapalo) clarification in Côte d\\'Ivoire was studied using the method of the experimental designs. Of the three factors selected that are the volume of mucilage (X1), the temperature (X2) and the pH (X3), sole X1 and X3 ...

  7. Response of Okra (Abelmoschus esculentus) varieties to NPK ...

    Pot and field experiments were conducted to determine the response of three okra varieties to four NPK fertilizers. The okra varieties; V35 (in the pot experiment and Clemson spineless in the field trial), Jokoso, and Sologo formed the main plot treatments and four NPK fertilizer rates: no fertilizer (control), 30 +15 +15, 60 +30 ...

  8. Regeneration of okra (Abelmoschus esculentus L.) via apical shoot ...

    USER

    2012-10-25

    Oct 25, 2012 ... esculentus L. Monech) N-550 line evolved at R&D, Nirmal Seeds Pvt. Ltd., was used as basic material ... Therefore, development of tissue culture protocols to induce .... Department of Botany, Rajshahi University, Bangladesh.

  9. Response of okra [ Abelmoschus esculentus (L.) Moench] to water ...

    Parameters such as relative water content, membrane permeability, chlorophyll content and yield of capsules varieties were evaluated. Our results show that water deficiency reduces relative water content of okra leaves. This reduction is more pronounced when water lack occurs at flowering stage of plants and, leakage of ...

  10. Antiadhesive Properties of Abelmoschus esculentus (Okra) Immature Fruit Extract against Helicobacter pylori Adhesion

    Shevtsova, Anna; Glocker, Erik; Borén, Thomas; Hensel, Andreas

    2014-01-01

    Background Traditional Asian and African medicine use immature okra fruits (Abelmoschus esculentus) as mucilaginous food to combat gastritis. Its effectiveness is due to polysaccharides that inhibit the adhesion of Helicobacter pylori to stomach tissue. The present study investigates the antiadhesive effect in mechanistic detail. Methodology A standardized aqueous fresh extract (Okra FE) from immature okra fruits was used for a quantitative in vitro adhesion assay with FITC-labled H. pylori J99, 2 clinical isolates, AGS cells, and fluorescence-activated cell sorting. Bacterial adhesins affected by FE were pinpointed using a dot-blot overlay assay with immobilized Lewisb, sialyl-Lewisa, H-1, laminin, and fibronectin. 125I-radiolabeled Okra FE polymer served for binding studies to different H. pylori strains and interaction experiments with BabA and SabA. Iron nanoparticles with different coatings were used to investigate the influence of the charge-dependence of an interaction on the H. pylori surface. Principal findings Okra FE dose-dependently (0.2 to 2 mg/mL) inhibited H. pylori binding to AGS cells. FE inhibited the adhesive binding of membrane proteins BabA, SabA, and HpA to its specific ligands. Radiolabeled compounds from FE bound non-specifically to different strains of H. pylori, as well as to BabA/SabA deficient mutants, indicating an interaction with a still-unknown membrane structure in the vicinity of the adhesins. The binding depended on the charge of the inhibitors. Okra FE did not lead to subsequent feedback regulation or increased expression of adhesins or virulence factors. Conclusion Non-specific interactions between high molecular compounds from okra fruits and the H. pylori surface lead to strong antiadhesive effects. PMID:24416297

  11. Effect of polyamines on biochemical properties in gamma irradiated Okra (Abelmoschus esculentus L.) varieties

    Bhushan, Himanshu; Shukla, Pradeep K.; Ramteke, Pramod W.; Misra, Pragati

    2014-01-01

    Gamma rays are electromagnetic radiations and can be useful for the alteration of physiological characters. In plant systems, gamma ray induced free radicals can damage or modify important components of plant cells and also modulate the morphological, anatomical and biochemical characters of the cell and the plant therein. Polyamine possesses protective role against unfavorable conditions because of its highest positive charge due to presence of amine groups. In plants polyamines are involved in organ development, flowering, fruit ripening, and senescence and stress responses. Okra (Abelmoschus esculentus L.) has captured a prominent position among vegetables and in India; it is grown on an area of 3.6 lakh hectares with a production of 34.2 lakh tones. Keeping these facts in mind, an experiment was conducted to study the effect of polyamines on biochemical properties in gamma irradiated Okra (Abelmoschus esculentus L.) varieties. Seeds of four Okra varieties (namely Kaveri 49, Kaveri 54, Deepika and OH2324) were expose to different levels of gamma radiation (T 1 = 200 Gy, T 2 = 400 Gy, T 3 = 600 Gy, and T 4 = 800 Gy) using 60 Co as source at National Botanical Research Institute (NBRI) research institute of CSIR at Lucknow. These plants were also subjected to an exogenous application of 1 mM spermine and 1 mM spermidineas foliar spray. The results showed that application of Polyamines (spermine and spermidine) increased Chlorophyll α content, Chlorophyll b content (mg/g fresh weight) in gamma treated plants as compared to control. Application of polyamines also increased enzymatic and non-enzymatic antioxidants (proline, ascorbte peroxidase and glutathione reductase) level in gamma treated plants as compared to control. Carotenoid and protein content showed variations under polyamine treatment. In general, variety Deepika was relatively tolerant to gamma radiation among all the varieties, whereas, Kaveri 49 and Kaveri 54 were relatively sensitive to gamma

  12. Genetic relationships among okra (Abelmoschus esculentus (L. Moench cultivars in Nigeria

    Bashir O. Bello

    2017-09-01

    Full Text Available This study was conducted on okra (Abelmoschus esculentus (L. Moench cultivars at the Teaching and Research Farm, University of Maiduguri, Nigeria. The objective was to evaluate the level of genetic divergence and heritability of eight characters in 2015 and 2016 dry seasons using irrigation. The results showed highly significant (p<0.01 differences in the ten okra cultivars for days to anthesis, plant height, fresh capsule length, fresh mass per capsule and fresh capsule diameter across the two years. A high genotypic coefficient of variation, heritability, and genetic advance were detected in all the characters except for days to anthesis and fresh capsule diameter. This implied that different genetic constitution and preponderance of additive effects governed these characters, thus presenting a significant opportunity for selection. The Mahanalobis D2 analysis allotted the ten cultivars into four clusters. The highest was cluster I comprising four cultivars, followed by cluster II containing three cultivars, cluster III consisting two cultivars, and cluster IV with mono genotypic. The three most superior okra cultivars (Salkade, Y’ar gagure and Kwadag for yield and related characters could be exploited directly or introgressed with other promising ones in future breeding programs.

  13. Anti hypoxic and antioxidant activity of Hibiscus esculentus seeds

    Ebrahimzadeh, M. A.; Nabavi, S. F.; Nabavi, S. M.; Eslami, B.

    2010-07-01

    The anti hypoxic and antioxidant activities of Hibiscus esculentus seeds were investigated employing eight in vitro assay systems. Anti hypoxic activity was investigated in two models, haemic and circulatory. The effects were pronounced in both models of hypoxia. The anti hypoxic effects were dose-dependent. The results indicated that the extracts have a protective effect against hypoxia induced lethality in mice. The extracts showed antioxidant activity in some models. IC{sub 5}0 for DPPH radical-scavenging activity was 234 {+-} 8.9 {mu}g ml{sup 1}. The extracts showed weak nitric oxide-scavenging activity between 0.1 and 1.6 mg ml{sup -}1. The extracts showed weak Fe{sup 2}+ chelating ability. IC{sub 5}0 were 150 {+-} 13 {mu}g ml{sup -}1. The extracts also exhibited low antioxidant activity in the linoleic acid model but were capable of scavenging hydrogen peroxide in a concentration dependent manner. The total amount of phenolic compounds in each extract was determined as gallic acid equivalents and total flavonoid contents were calculated as quercetin equivalents from a calibration curve. Pharmacological effects may be attributed, at least in part, to the presence of phenols and flavonoids in the extracts. (Author) 40 refs.

  14. Antihypoxic and antioxidant activity of Hibiscus esculentus seeds

    Eslami, Bahman

    2010-03-01

    Full Text Available The antihypoxic and antioxidant activities of Hibiscus esculentus seeds were investigated employing eight in vitro assay systems. Antihypoxic activity was investigated in two models, haemic and circulatory. The effects were pronounced in both models of hypoxia. The antihypoxic effects were dose-dependent. The results indicated that the extracts have a protective effect against hypoxia induced lethality in mice. The extracts showed antioxidant activity in some models. IC50 for DPPH radical-scavenging activity was 234 ± 8.9 μg ml-1. The extracts showed weak nitric oxide-scavenging activity between 0.1 and 1.6 mg ml-1. The extracts showed weak Fe2+ chelating ability. IC50 were 150 ± 13 μg ml-1. The extracts also exhibited low antioxidant activity in the linoleic acid model but were capable of scavenging hydrogen peroxide in a concentration dependent manner. The total amount of phenolic compounds in each extract was determined as gallic acid equivalents and total flavonoid contents were calculated as quercetin equivalents from a calibration curve. Pharmacological effects may be attributed, at least in part, to the presence of phenols and flavonoids in the extracts.La actividad antihipóxica y antioxidante de semillas de Hibiscus esculentus fue investigada empleando ocho ensayos in vitro. La actividad antihipóxica fue investigada en dos modelos, uno de caracter hemínico y otro circulatorio. Los efectos fueron pronunciados en ambos modelos de hipoxia. Los efectos antihipóxicos fueron dependientes de la dosis. Los resultados indican que los extractos tienen un efecto protector contra la letabilidad inducida por hipoxia en ratones. Los extractos mostraron actividad antioxidante en algunos modelos. El IC50 para la actividad captadora de radicales fue 234 ± 8.9 μg ml-1. Los extractos muestran una débil actividad captadora de óxido nítrico comprendida entre 0.1 y 1.6 mg ml-1. Los extractos muestran una débil capacidad quelatante de Fe2+. El IC

  15. Assessment of genetic diversity in okra (abelmoschus esculentus l.) using rapd markers

    Haq, I.; Khan, A.A.; Azmat, M.A.

    2013-01-01

    Thirty nine okra genotypes were assessed for genetic variability using Random Amplified Polymorphic DNA (RAPD) markers. Twenty polymorphic RAPD primers amplified 111 DNA fragments, with an average of 5.5 fragments per primer. Among 39 okra genotypes, 107 fragments (96%) were found to be polymorphic. The UPGMA cluster analysis placed okra genotypes into seven main clusters. Sabzpari 2001 and Acc. No. 019221 had shown maximum similarity (83%) while the minimum similarity (44.14%) was observed between the genotypes Punjab Selection and Acc. No. 019217. Thus, by using RAPD primers a considerable polymorphism appeared to exist, which showed genetic variability in the okra genotypes. (author)

  16. The Preotective effects of Okra Powder (Abelmoschus esculentus on Histological and Histochemical Changes of Pancreatic Beta Cells and Liver Tissue of Diabetic Rat

    Naeim Erfani Majd

    2017-04-01

    Full Text Available Background and Objective: Since Abelmoschus esculentus plant has many medical benefits, the present study aimed to investigate the therapeutic effects of Okra Powder (Abelmoschus esculentus against high-fat diet fed-streptozotocin (HFD/STZ-induced diabetic rats. Methods: In this experimental study, 25 Wistar Albino female rats were randomly divided into 5 groups: I: control group; II: healthy rats receiving A. esculentus (200 mg/kg; III (HFD/STZ group: Rats fed with high-fat diet (HFD (60% fat for 4 weeks and then injected low-dose STZ (35 mg/kg; IV: diabetic rats receiving A. esculentus (200mg/kg and V: diabetic rats receiving metformin (200 mg/kg. At the end of experiment, biochemical parameters, including Fasting Blood Glucose (FBG, insulin levels, Homa-IR index, ALT, AST and lipid profile were measured. Pancreas and Liver samples were removed, and 5-6 µ sections were prepared and stained by H&E and aldehyde fuchsin staining. Results: All the biochemical parameters, except HDL-C and insulin, were increased in diabetic rats, while these parameters were decreased in Okra supplementation group compared to diabetic rats (p<0.05. Furthermore, Okra improved the histological impairments of pancreas and liver, including vacuolization, and decrease of β-cells as well as hypertrophy and vacuolization of hepatocytes in diabetic rats. Conclusion: Okra powder improved biochemical parameters, liver structure and restoration of beta cells of pancreas in diabetic rats. Thus, it can be considered a complementary therapy to improve diabetic patients.

  17. Decontamination of sliced and powdered okra (Abelmoschus esculentus) and some aspects of nutrient quality before and after gamma irradiation

    Ofori-Appiah, D.

    2012-01-01

    Food security in Africa is crucial for survival of the increasing population. However, agricultural produce in the field are drastically reduced along the food pipeline (from farm gate to the consumer's table) by bioderioagents including microorganisms. Okra (Abelmoschus esculentus (L) Moench) is one such farm produce of economic importance in Ghana with a great potential of providing essential nutrients and vitamins in our diet. The high moisture content of the fresh fruit makes it susceptible to microbial deterioration in a short time. Dehydration methods (sun-drying and solar drying) are practiced worldwide but in Africa, this is attended by contamination by aeromycoflora and other agents such as insect eggs and larvae. In addition are physical and chemical contaminants. In this thesis, the mycoflora and Total Aerobic Bacteria load of market samples and solar-dried okra (Clemson spineless and Legon Finger) chips and powder were ascertained with the view to documenting toxin-producing fungal species and update the resident mycoflora and bacteria load. Insects resident in the samples were detected by the hidden infestation technique; mycoflora was determined by the decimal serial dilution method on different media and Total Aerobic Bacteria population was determined on Plate Count Agar at 320C for 48hr. In order to establish storage stability of the okra, the chips and powdered samples were placed in glass desiccators with glycerol: water mixtures providing Environmental Relative Humidities of 20, 55, 65, 75, 85 and 95% representative of the Ghanaian Tropic Conditions to undergo sorption at the same temperature. Gamma irradiation doses (0, 5, 10, 20kGy) were used as a preservation process to decontaminate resident mycoflora and total aerobic bacteria. This was supplemented by an in vitro study in the radio-resistance of six selected resident fungi (Aspergillus; Penicllium spp). The veracity of the dry okra supporting growth of selected Aspergillus and Penicillium

  18. Physiological and biochemical response to high temperature stress in Okra (Abelmoschus esculentus L. Moench)

    Hayamanesh, Shahnoosh; Keitel, Claudia; Ahmad, Nabil; Trethowan, Richard

    2016-04-01

    High temperature has been shown to lower the growth and yield of Okra, an important summer vegetable crop grown in Asia, Africa, the Middle East and Australia. We aimed to characterise the physiological and biochemical response of Okra to heat stress. 150 genotypes from Pakistan and the AVRDC (The World Vegetable Centre) were screened for their physiological response (fluorescence, electrolyte leakage and yield) to heat in a greenhouse. Four genotypes (including heat tolerant and sensitive) were selected and subsequently grown in control and hot greenhouses. Daytime temperatures were on average 10°C warmer in the hot greenhouse, whereas nighttime temperatures were similar between the two temperature treatments. During a 12 week period, the physiological (assimilation rate, transpiration rate, stomatal conductance, fluorescence, electrolyte leakage, water potential) and biochemical (carbohydrates, sugar alcohols, C content) response of the four genotypes to heat stress was assessed. The effect of heat stress on the C allocation patterns and yield in Okra will be discussed.

  19. Characterisation of cell wall polysaccharides from okra (Abelmoschus esculentus (L.) Moench)

    Sengkhamparn, N.; Verhoef, R.P.; Schols, H.A.; Sajjaanantakul, T.; Voragen, A.G.J.

    2009-01-01

    Okra pods are commonly used in Asia as a vegetable, food ingredient, as well as a traditional medicine for many different purposes; for example, as diuretic agent, for treatment of dental diseases and to reduce/prevent gastric irritations. The healthy properties are suggested to originate from the

  20. Genetic basis of variation for salinity tolerance in okra (abelmoschus esculentus L.)

    Ikram-ul-Haq; Khan, A.A.; Azhar, F.M.; Ullah, E.

    2010-01-01

    The development of salt tolerant plants through selection and breeding depends on the presence of the genetic variability within the crop species in response to salt stress, which must have significant genetic component. Such information is not extensively available in vegetable crops. The present study was carried out to gain some information on the genetic basis of variation for salinity tolerance in okra. North Carolina Mating Design II (NCM II) was used for the estimation of genetic components of variation in the traits affecting salinity tolerance. The inheritance of the traits affecting salinity tolerance at the seedling stage appeared to be controlled by both additive and non-additive effects (dominance and epistasis). The narrow sense heritability estimates ranged from 40 to 65% and 7 to 70% and the estimates of broad sense heritability ranged from 65 to 99% and 20 to 99% for absolute and relative values. The additive effects were relatively more prominent and narrow sense heritability was moderate. The high additive component for absolute Na/sup +/ and K/sup +//Na/sup +/ ratio at 60 and 80 mM NaCl, relative Na+ at 80 mM NaCl suggested that improvement for salinity tolerance in okra would be possible on the basis of these characteristics through selection and breeding. The genetic variation for tolerance to NaCl salinity existed among the okra genotypes, which had considerable heritable component and, therefore, genetic improvement of okra genotypes for salinity tolerance through recurrent selection method is possible. (author)

  1. ANALISA FLAVONOID DARI EKSTRAK ETANOL 96% KULIT BUAH OKRA MERAH (Abelmoschus esculentus L. Moench SECARA KROMATOGRAFI LAPIS TIPIS DAN SPEKTROFOTOMETRI UV-VIS

    Nia Lisnawati

    2016-03-01

    Full Text Available Has done research on flavonoids Analysis of Ethanol Extract 96% Fruit Leather Red Okra In Thin Layer Chromatography and Spectrophotometer UV-Vis. The purpose of this study was to analyze the content of the fruit skin red okra (Abelmoschus esculentus L. Moench by using the method of thin layer chromatography (TLC under UV light and spectrophotometry UV-Vis. Reference standards used in this study is the Standard Solution Routine Quercetin. The results of the research that has been done by the method of thin layer chromatography obtained Rf values of 0.81 and produces the color orange. And the results of research conducted by spectrophotometry UV-Vis method obtained 333,117 mg.L-1 or 421,629 mg.kg-1 or 0,84339 %. The conclusion from this study is that the 96% ethanol extract of the fruit leather red okra (Abelmoschus esculentus L. Moench positive (+ contains flavonoids with levels of 0,84339 %.

  2. Genetic variability and heritability in cultivated okra [Abel moschus esculentus (L.) Moench

    Nwangburuka, C. C.; Denton, O. A.; Khinde, O. B.; Ojo, D. K.; Popoola, A. R.

    2012-11-01

    Twenty-nine okra accessions from different agro-ecological regions in Nigeria were grown during the rainy and dry seasons, between 2006 and 2007 at Abeokuta (derived savanah) and Ilishan (rainforest) and assessed to determine their genetic variability, heritability and genetic advance from eight yield related characters. The experiment was laid out in a Randomized Complete Block Design with five replications. There was high genotypic coefficient of variability, % broad-sense heritability and genetic advance in traits such as plant height (26.2, 90.7, 51.5), fresh pod length (23.9, 98.5, 48.8), fresh pod width (23.9, 98.5, 48.8), mature pod length (28.6, 98.5, 52.3), branching per plant (29.3, 82.3, 54.8) and pod weight per plant (33.9, 90.0, 63.3), suggesting the effect of additive genes and reliability of selection based on phenotype of these traits for crop improvement. The positive and significant phenotypic and genotypic correlation between plant height at maturity, fresh pod width, seeds per pod and pods per plant, branches per plant with seed weight per plant and pod weight per plant, suggests that selection on the basis of the phenotype of these characters will lead to high seed and pod yield in okra. (Author) 26 refs.

  3. MODE OF INHERITANCE OF POD SPININESS IN OKRA (Abelmoschus esculentus (L. Moench

    Adil Hassan Ahmed Abdelmageed

    2009-10-01

    Full Text Available The mode of inheritance of spininess in okra was investigated. Two okra cultivars, namely ‘Khartoumia spiny’ and the Indian cultivar ‘Pusa Sawani’ were used in this study. The two parents were self pollinated for three successive generations to fix the character under study. Crosses were made between ‘Khartoumia spiny’ and ‘Pusa Sawani’, and reciprocal F1’s, F2’s and all possible backcrosses were derived from the initial crosses. No reciprocal differences were found between F1 and F2 generation for pod spininess. Segregation in the crosses between the local cultivar ‘Khartoumia spiny’ and the Indian cultivar ‘Pusa Sawani’ indicated that the presence of spines on pods was controlled by single gene, with incomplete dominance.

  4. Abelmoschus esculentus

    Pedro

    2015-02-16

    Feb 16, 2015 ... Author(s) agree that this article remains permanently open access under the terms of the Creative Commons .... assumptions of parametric tests and obtain consistent results, the .... Ladies Finger (Abelmoschus esculentus L.) in Mice. Sci. ... and okra mosaic viral diseases, under field conditions in South.

  5. Advances in breeding of okra [Abelmoschus esculentus (L.) Moench.] in India

    Sharma, B R; Arora, S K [Department of Vegetable Crops, Landscaping and Floriculture, Punjab Agricultural University, Ludhiana (India)

    1990-01-01

    Full text: Okra, an important vegetable of the tropics and sub-tropics is very popular in India. Its production is limited by 'yellow vein mosaic virus'. Advances in breeding for resistance to this virus have been made through inter-specific hybridisation as well as mutagenesis. Hybridisation used A. manihot ssp. manihot and ssp. tetraphyllus. New varieties showed 87-146% yield increases over older virus susceptible varieties. The number of fruits increased by 13-30%, virus incidence decreased by 84-99%. An EMS induced mutant 'EMS8' showed a yield increase of 107%, a fruit number increase of 16% and a disease decrease of 99%. The mutant also carries a good amount of resistance to the fruit borer; infestation decreased by 46%. The mutant is the best among the tested varieties for canning, is suitable for dehydration, and can be stored prepacked at room temperature for 6 days. (author)

  6. Evaluation of Fatty Acid and Amino Acid Compositions in Okra (Abelmoschus esculentus Grown in Different Geographical Locations

    Rokayya Sami

    2013-01-01

    Full Text Available Okra has different uses as a food and a remedy in traditional medicine. Since it produces many seeds, distribution of the plant is also quite easy. Although seed oil yield is low (4.7%, since the linoleic acid composition of the seed oil is quiet high (67.5%, it can still be used as a source of (UNSAT unsaturated fatty acids. In this study, samples of okra grown in four different locations were analyzed to measure fatty acid and amino acid compositions. The content of the lipid extraction ranged from 4.34% to 4.52% on a dry weight basis. Quantitatively, the main okra fatty acids were palmitic acid (29.18–43.26%, linoleic acid (32.22–43.07%, linolenic acid (6.79–12.34%, stearic acid (6.36–7.73%, oleic acid (4.31–6.98%, arachidic acid (ND–3.48%, margaric acid (1.44–2.16%, pentadecylic acid (0.63–0.92%, and myristic acid (0.21–0.49%. Aspartic acid, proline, and glutamic acids were the main amino acids in okra pods, while cysteine and tyrosine were the minor amino acids. Statistical methods revealed how the fatty acid and amino acid contents in okra may be affected by the sampling location.

  7. Field efficacy of azadirachtin-A, tetrahydroazadirachtin-A, NeemAzal and endosulfan against key pests of okra (Abelmoschus esculentus).

    Dhingra, Swaran; Walia, Suresh; Kumar, Jitendra; Singh, Shivendra; Singh, Gyanendra; Parmar, Balraj S

    2008-11-01

    BACKGROUND Unlike synthetic pesticides, azadirachtin-based neem pesticides are environmentally friendly and are well known for their diverse pest control properties. Their use is, however, limited by the instability of azadirachtin, necessitating application at short intervals. The efficacy of relatively stable tetrahydroazadirachtin-A, therefore, needed to be established under field conditions. Azadirachtin-A (Aza-A), its stable derivative tetrahydroazadirachtin-A (THA) and other neem pesticides have been evaluated for their field efficacy against major insect pests of okra, Abelmoschus esculentus (L.) Moench., during summer (kharif) 2003 and 2004. The optimum doses of Aza-A and THA against the fruit borer, Earias vittella F., were also established. Reductions in population of whitefly, Bemisia tabaci (Genn.), and leafhopper (jassid), Amrasca biguttulla biguttulla Ishida, after application of THA or endosulfan was evident up to 10 days after treatment (DAT), whereas with Aza-A and NeemAzal (NZ) the effect was observed up to 7 DAT only. Endosulfan and THA also caused higher reduction in the larvae of shoot and fruit borer E. vittella and E. insulana Boisd., and recorded the highest yields of 4600 and 4180 kg ha(-1). The efficacy of THA (0.05 g L(-1) emulsion) was comparable with that of 0.5 g L(-1) endosulfan emulsion in reducing fruit borer infestation, the reduction over the control being 86.0 and 87.3%, 84.9 and 94.1% and 90.2 and 92.6% at first, second and third picking. THA 0.02 g L(-1) and Aza-A 0.05 g L(-1) were on a par. Laboratory-made neem oil emulsifiable concentrate was the least effective, but was superior to untreated check. Three consecutive sprays of THA, a neem-based biopesticide, and endosulfan have been found to be superior in controlling field pests of okra to Aza-A and NZ, which were on a par. THA thus holds potential as a component of pest management strategies against okra pests. Copyright (c) 2008 Society of Chemical Industry.

  8. Effect of Meloidogyne arenaria and Mulch Type on Okra in Microplot Experiments.

    Ritzinger, C H; McSorley, R; Gallaher, R N

    1998-12-01

    The effects of perennial peanut (Arachis glabrata) hay, an aged yard-waste compost (mainly woodchips), and a control treatment without amendment were determined on two population levels of root-knot (Melaidogyne arenaria) nematode over three consecutive years in field microplots. Okra (Hibiscus esculentus, susceptible to the root-knot nematode) and a rye (Secale cereale) cover crop (poor nematode host) were used in the summer and winter seasons, respectively. The organic amendment treatments affected plant growth parameters. In the first year, okra yields were greatest in peanut-amended plots. Yield differences with amendment treatment diminished in the second and third years. Okra plant height, total fruit weight, and fruit number were greater with the lower population level of the root-knot nematode. Residual levels of nutrients in soil were greater where root-knot nematode levels and damage were higher and plant growth was poor. Nutrient levels affected the growth of a subsequent rye cover crop.

  9. Laboratory evaluation of interception and translocation of 131I in fenugreek and Okra plants

    Singhal, R.K.; Narayanan, U.; Bhat, I.S.

    1994-01-01

    The work reported here deals with the study of interception and translocation of airborne 131 I in fenugreek (Trigonella foenum-graecum) and okra (Hibiscus esculentus), two very common vegetables in India. Activity was injected into the experimental chamber in the form of iodide aerosols having a size distribution of 0.3 to 5.0 μm (AMAD). Samples of plants were collected over a period of a few days at different time intervals after injection of the aerosols. Evaluation of interception with deposition and translocation of 131 I was done from the activity measured in air and in plant parts. For the deposition factor, the values are 1.22 m 3 kg -1 for fenugreek leaves, and 1.49 m 3 kg -1 for the plant as a whole. For okra plants these values are 0.02 and 0.16 m 3 kg -1 in edible okra and leaves, respectively. For the okra plant as a whole, the value is 0.19 m 3 kg -1 . The translocation factors vary from 0.62 to 0.86 and 0.47 to 0.87 for leaves and stem, respectively, in the case of fenugreek plants. For okra plants, the translocation factor varies from 0.21 to 0.82, 0.53 to 0.93, and 0.42 to 0.81 in edible okra, leaves, and stems, respectively. 13 refs., 6 figs., 2 tabs

  10. Processing Effects on the Antioxidant Activities of Beverage Blends Developed from Cyperus esculentus, Hibiscus sabdariffa, and Moringa oleifera Extracts.

    Badejo, Adebanjo A; Damilare, Akintoroye; Ojuade, Temitope D

    2014-09-01

    The discovery of bioactive compounds in foods has changed the dietary lifestyle of many people. Cyperus esculentus (tigernut) is highly underutilized in Africa, yet tigernut extract is highly profitable in Europe. This study aims to add value to tigernut extract by revealing its health benefits and food value. In this study, tigernut tubers were germinated or roasted and the extracts were combined with Moringa oleifera extract (MOE) or Hibiscus sabdariffa extract (HSE) and spiced with ginger to produce functional drinks. The drinks were evaluated for physicochemical characteristics, sensory parameters, and antioxidant potentials. The total phenolic content of each beverage was measured by the Folin-Ciocalteu method, and the antioxidant activity of each beverage was determined by the 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid assays. The beverages from the germinated tigernut extracts had the highest titratable acidity and the lowest pH, while beverages containing the roasted tigernut extract had the highest ∘Brix. Germination and roasting significantly enhanced the total phenolic content of the drinks. The beverage containing HSE and germinated tigernut extract had a total phenolic content of 45.67 mg/100 mL gallic acid equivalents, which was significantly higher than the total phenolic content of all other samples. The DPPH inhibition activity of the beverages prepared with germinated tigernut extracts was significantly higher than the DPPH inhibition activity of the beverages prepared with fresh tigernut extract. The taste and overall acceptability of drinks containing the roasted tigernut extract were preferred, while the color and appearance of drinks with the germinated samples were preferred. Roasting or germinating tigernuts before extraction and addition of MOE or HSE extracts is another way to add value and enhance the utilization of tigernuts.

  11. Processing Effects on the Antioxidant Activities of Beverage Blends Developed from Cyperus esculentus, Hibiscus sabdariffa, and Moringa oleifera Extracts

    Badejo, Adebanjo A.; Damilare, Akintoroye; Ojuade, Temitope D.

    2014-01-01

    The discovery of bioactive compounds in foods has changed the dietary lifestyle of many people. Cyperus esculentus (tigernut) is highly underutilized in Africa, yet tigernut extract is highly profitable in Europe. This study aims to add value to tigernut extract by revealing its health benefits and food value. In this study, tigernut tubers were germinated or roasted and the extracts were combined with Moringa oleifera extract (MOE) or Hibiscus sabdariffa extract (HSE) and spiced with ginger to produce functional drinks. The drinks were evaluated for physicochemical characteristics, sensory parameters, and antioxidant potentials. The total phenolic content of each beverage was measured by the Folin-Ciocalteu method, and the antioxidant activity of each beverage was determined by the 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid assays. The beverages from the germinated tigernut extracts had the highest titratable acidity and the lowest pH, while beverages containing the roasted tigernut extract had the highest ∘Brix. Germination and roasting significantly enhanced the total phenolic content of the drinks. The beverage containing HSE and germinated tigernut extract had a total phenolic content of 45.67 mg/100 mL gallic acid equivalents, which was significantly higher than the total phenolic content of all other samples. The DPPH inhibition activity of the beverages prepared with germinated tigernut extracts was significantly higher than the DPPH inhibition activity of the beverages prepared with fresh tigernut extract. The taste and overall acceptability of drinks containing the roasted tigernut extract were preferred, while the color and appearance of drinks with the germinated samples were preferred. Roasting or germinating tigernuts before extraction and addition of MOE or HSE extracts is another way to add value and enhance the utilization of tigernuts. PMID:25320721

  12. EFFECT OF THE ADDITION OF DEFATTED OKRA SEED (Abelmoschus esculentus FLOUR ON THE CHEMICAL COMPOSITION, FUNCTIONAL PROPERTIES AND Zn BIOAVAILABILITY OF PLANTAIN (Musa paradisiacal Linn FLOUR

    Tesleem Ibrahim

    2012-08-01

    Full Text Available In Nigeria it is advised that nursing mothers should give their baby plantain flour paste “amala ogede” with ‘ewedu’ Corchorus olitorius soup during the process of weaning their baby. The over matured okra that cannot be cut with kitchen knife are thrown away in Nigeria, this lead to postharvest loss of okra. The seed in this okra could be utilised by processing into okra seed flour for the fortification of plantain flour. Since the okra seed flour is rich in oil and the oil contains cyclopropenoid fatty acids which cause some toxicity concerns therefore this work is to evaluate the chemical composition, the functional properties and Zn bioavailability of plantain flour mixed with defatted okra seed flour. The nutrient content increased significantly (P≤0.05, protein; 3.88 – 11.38 %, fibre; 3.03 – 16.30 % and ash; 2.72 – 5.77 % while the fat and carbohydrate content reduced significantly (P≤0.05 as the percentage of defatted okra seed flour increased. The bulk density of the plantain flour decreased significantly (P≤0.05 from 0.795 g/cm3 to 0.769 g/cm3 as the percentage of okra seed flour increased while the least gelation concentration increased significantly (P≤0.05 (10 to 20. The calculated [Ca][Phytate]/[Zn] molar ratio for the plantain flour mixed with defatted okra seed flour (0.02 – 0.04 mol/kg were below the critical level. The increase in the least gelation concentration coupled with increase in the protein content of the resultant flour from the blend means more of the protein will be available in the food made from the plantain flour mixed with defatted okra seed flour.

  13. Efficient genetic transformation of okra (Abelmoschus esculentus (L.) Moench) and generation of insect-resistant transgenic plants expressing the cry1Ac gene.

    Narendran, M; Deole, Satish G; Harkude, Satish; Shirale, Dattatray; Nanote, Asaram; Bihani, Pankaj; Parimi, Srinivas; Char, Bharat R; Zehr, Usha B

    2013-08-01

    Agrobacterium -mediated transformation system for okra using embryos was devised and the transgenic Bt plants showed resistance to the target pest, okra shoot, and fruit borer ( Earias vittella ). Okra is an important vegetable crop and progress in genetic improvement via genetic transformation has been impeded by its recalcitrant nature. In this paper, we describe a procedure using embryo explants for Agrobacterium-mediated transformation and tissue culture-based plant regeneration for efficient genetic transformation of okra. Twenty-one transgenic okra lines expressing the Bacillus thuringiensis gene cry1Ac were generated from five transformation experiments. Molecular analysis (PCR and Southern) confirmed the presence of the transgene and double-antibody sandwich ELISA analysis revealed Cry1Ac protein expression in the transgenic plants. All 21 transgenic plants were phenotypically normal and fertile. T1 generation plants from these lines were used in segregation analysis of the transgene. Ten transgenic lines were selected randomly for Southern hybridization and the results confirmed the presence of transgene integration into the genome. Normal Mendelian inheritance (3:1) of cry1Ac gene was observed in 12 lines out of the 21 T0 lines. We selected 11 transgenic lines segregating in a 3:1 ratio for the presence of one transgene for insect bioassays using larvae of fruit and shoot borer (Earias vittella). Fruit from seven transgenic lines caused 100 % larval mortality. We demonstrate an efficient transformation system for okra which will accelerate the development of transgenic okra with novel agronomically useful traits.

  14. Effect of electromagnetic field on okra (Hibiscus sculentus L. developmental stages and the effect of okra extract on breast cancer cells

    Z. Hasani Sadrabadi

    2016-04-01

    Full Text Available Background and objectives: Electric, magnetic and electromagnetic fields can act as stress factors with different effects on biological systems. Due to the nutritional and medicinal values, and the increasing electromagnetic radiations, the present study was performed to investigate the effects of the electromagnetic field on the developmental stages and cytotoxic properties of okra. Methods: Both dry and wet seeds were exposed to electromagnetic field with the intensities of 2 and 4 mT for 60 minutes. MTT assay was applied to evaluate the potential cytotoxic effects of okra extract on MCF-7 cell line. The anatomical structure of leaves in both treated and untreated (control plants were examined. Results: The results showed that a field intensity of 4 mT increased the speed of germination of wet treated seeds and the stem length of dry treated seeds. MTT assay revealed no cytotoxicity of the aqueous extracts of okra pods up to the concentration of 100 μg/mL from either the treated or the control plants towards the MCF-7 cell line. Conclusion: The results suggest that the electromagnetic fields would be able to increase the speed of germination without effects on percentage of germination.

  15. Study of Okra Powder (Abelmoscus Esculentus Effects on Histology of Liver Tissue and Sero-Biochemical Parameters in Diabetic Rats (HFD/STZ

    N erfani majd

    2016-12-01

    Full Text Available Introduction: Type 2 diabete is a kind of metabolic disease that it is associated with hyperglycemia, hyperlipidemia and disturbed liver function.  The aim of the present study was to evaluate the protective effects of Okra Powder on liver damage in high fat diet fed / streptozotocin (HFD-STZ-induced type 2 diabetic rats. Methods: In this experimental study, 25 Wistar Albino female rats with an average weight of (200–220 g were randomly divided  into 5 groups: Group I: (control group rats were fed the standard diet, Group II: healthy rats that received Okra Powder (200 mg/kg for 4 weeks; Group III (HFD/STZ group: Rats were fed with high-fat diet (HFD (60% fat for 4 weeks  and then injected low dose of STZ (35 mg/kg, Group IV:  Diabetic rats that received Okra Powder (200 mg/kg for 4 weeks. GroupV: Diabetic rats that received metformin (200 mg/kg for 4 weeks. At the end of experiment, biochemical parameters were measured. Liver samples were removed and 5-6 µ sections were made and stained by H&E and Sudan black staining. Results: The results showed that all the biochemical parameters, except HDL-C and serum insulin were increased in diabetic rats, while they were decreased in Okra supplementation group compared  to diabetic rats (p<0.05. The liver structure alterations were improved in treated diabetic rats with Okra Powder and metformin.  Conclusion: Our findings confirmed the potential anti-hyperglycemic and hypolipidemic effects of Okra Powder. Thus, it seems it has an important role in the management of type 2 diabete.

  16. Morphological classification of genetic diversity in cultivated okra ...

    Morphological classification of genetic diversity in cultivated okra, Abelmoschus esculentus (L) Moench using principal component analysis (PCA) and single linkage cluster analysis (SLCA). CC Nwangburuka, OB Kehinde, DK Ojo, OA Denton, AR Popoola ...

  17. Induction of Defense-Related Physiological and Antioxidant Enzyme Response against Powdery Mildew Disease in Okra (Abelmoschus esculentus L.) Plant by Using Chitosan and Potassium Salts.

    Soliman, Mona H; El-Mohamedy, Riad S R

    2017-12-01

    Foliar sprays of three plant resistance inducers, including chitosan (CH), potassium sorbate (PS) (C 6 H 7 kO 2 ), and potassium bicarbonates (PB) (KHCO 3 ), were used for resistance inducing against Erysiphe cichoracearum DC (powdery mildew) infecting okra plants. Experiments under green house and field conditions showed that, the powdery mildew disease severity was significantly reduced with all tested treatments of CH, PS, and PB in comparison with untreated control. CH at 0.5% and 0.75% (w/v) plus PS at 1.0% and 2.0% and/or PB at 2.0% or 3.0% recorded as the most effective treatments. Moreover, the highest values of vegetative studies and yield were observed with such treatments. CH and potassium salts treatments reflected many compounds of defense singles which leading to the activation power defense system in okra plant. The highest records of reduction in powdery mildew were accompanied with increasing in total phenolic, protein content and increased the activity of polyphenol oxidase, peroxidase, chitinase, and β-1,3-glucanase in okra plants. Meanwhile, single treatments of CH, PS, and PB at high concentration (0.75%, 2.0%, and/or 3.0%) caused considerable effects. Therefore, application of CH and potassium salts as natural and chemical inducers by foliar methods can be used to control of powdery mildew disease at early stages of growth and led to a maximum fruit yield in okra plants.

  18. Genetic and morphological diversity of okra (Abelmoschus esculentus [L.] Moench.) genotypes and their possible relationships, with particular reference to Greek landraces

    Kyriakopoulou, O.G.; Arens, P.F.P.; Pelgrom, K.T.B.; Karapanos, I.; Bebeli, P.; Passam, H.C.

    2014-01-01

    Despite its high economic value in many countries (especially in developing regions of the tropics and sub-tropics), okra has received little attention with respect to its source of origin and genetic diversity, particularly at the molecular level. Phenotypic description (morphology, pod

  19. Hibiscus

    ... uterus; reduce swelling; and work like antibiotics to kill bacteria and worms. ... Herbs and supplements that might lower blood pressureHibiscus may lower blood pressure. Using it along with other ...

  20. Characterization of the okra mucilage by interaction with Gal, GalNAc and GlcNAc specific lectins.

    Wu, A M; Jiang, Y J; Hwang, P Y; Shen, F S

    1995-02-23

    A bio-active polysaccharide, which was the major component of the extract of the common okra, Hibiscus esculentus, was isolated from the extract by precipitation with ethanol between 28.5 to 45%. According to a previous report (Whistler, R.L. and Conrad, H.E. (1954) J. Am. Chem. Soc. 76, 1673-1674), this polysaccharide contains the Gal alpha 1-->4Gal sequence, which is the ligand for the uropathogenic Escherichia coli and toxic lectins. Analysis of the binding property of the okra polysaccharide by precipitin assay with Gal, GalNAc and GlcNAc specific lectins showed that this okra mucilage reacted best with Mistletoe toxic lectin-I (ML-I) and precipitated over 80% of the ML-I nitrogen (5.1 micrograms N) added. It also precipitated well with Abrus precatorius (APA), Momordica charantia (MCA) and Ricinus communis (RCA1) agglutinins, but poorly with other lectins. The results obtained suggest that this polysaccharide is a valuable reagent to differentiate Gal specific lectins from the GalNAc and/or GlcNAc specific series.

  1. Effects of spacing on the growth and yield of Okra ( Abelmochus ...

    The effects of plant population on growth and yield of Okra (Abelmoschus esculentus L. Moench) was investigated for two planting seasons (2008 and 2009) in the teaching and research farm, Department of Agronomy, Cross River University of Technology, Obubra . Treatments were five Okra plant populations : 111,111, ...

  2. Okra (Abelmoschus esculentus Linn) inhibits lipopolysaccharide ...

    Tropical Journal of Pharmaceutical Research June 2017; 16 (6): 1285-1292 ... lipopolysaccharide-induced inflammatory mediators in .... Multiple comparison of data was carried out by one-way. ANOVA followed by Bonferroni post-tests. P <.

  3. Chemical, physical and biological features of Okra pectin

    Sengkhamparn, N.

    2009-01-01

    In Thailand, many plants have been used as vegetables as well as for traditional
    medicine. Okra, Abelmoschus esculentus (L.) Moench, is an example of such a plant.
    Examples for the medical use are treatment of gastric irritation, treatment of dental
    diseases, lowering cholesterol

  4. HPLC Analysis of Water-Soluble Vitamins (B2, B3, B6, B12, and C and Fat-Soluble Vitamins (E, K, D, A, and β-Carotene of Okra (Abelmoschus esculentus

    Rokayya Sami

    2014-01-01

    Full Text Available Okra is consumed as a vegetable by populations in Africa and Asia and particularly in Egypt. In this study, we investigated some nutritional components of okra grown in four different geographical locations of Egypt. A comparative analysis of water-soluble vitamins (B2, B3, B6, B12, and C and fat-soluble vitamins (E, K, D, A, and β-carotene in okra pods was carried out. Results of principal component analysis (PCA showed three clusters of varieties. The first cluster included the Dakahlia (D and Kafr El-Sheikh (K varieties. The second and the third clusters separated out the Suez (S and Mansoura (M varieties independently. The S pod showed the highest contents of vitamins B6 (49.81 μg/100 g and E (1.47 mg/100 g but contained the lowest contents of vitamins B3 (1.42 μg/100 g and B12 (undetected. The K pod showed the lowest vitamin C content (11.60 mg/100 g. The M pod showed the highest contents of vitamins B3 (22.70 μg/100 g, B12 (91.20 μg/100 g, C (27.14 mg/100 g, and K (0.21 mg/100 g. The D pod showed the lowest contents of vitamins E (0.15 mg/100 g, K (0.05 mg/100 g, and B6 (11.50 μg/100 g. These findings could help develop meal planning at the community level by incorporating okra varieties with high vitamin content.

  5. Efeito de alguns herbicidas na cultura do quiabeiro, em testes efetuados em casa de vegetação Effect of some herbicides on okra crop in trials performed in the greenhouse

    Aldo Alves

    1970-01-01

    Full Text Available Duas variedades de quiabeiro foram testadas, em condições de casa de vegetação, com solo de textura areno-barrenta, na sua tolerância para 16 herbicidas, cada qual aplicado em uma única dose. Dentre os herbicidas aplicados em pré-plantio incorporado o triflu-ralin destacou-se no combate às ervas daninhas gramíneas e não causou nenhum dano para a planta do quiabeiro. Os herbicidas cloroxuron, linuron, norea e propachlor mostraram ótimos resultados no cambate às ervas más e quanto à tolerância dessa malvácea. Do grupo das triazinas, todos os herbicidas usados nas condições do teste provocaram danos para o quiabeiro.Two okra (Hibiscus esculentus L. varieties were tested for their tolerance to sixteen herbicides at one rate each, as a pre-planting and as a pre-emergence applications in greenhouse trials. The results with the incorporated herbicides showed that only tri-fluralin at 0,75 kg/ha active ingredient was promising to this crop. Different results were obtained with the pre-emergence applications of the group of urea and anilide herbicides. While no symptoms of injury were observed with chloroxuron, norea, linuron, and propachlor, injury symptoms were evident with diuron, fluometuron, metobromuron and siduron. The four triazine herbicides were very damaging to the okra plants. Under the conditions of these tests, only the herbicides trifluralin, chloroxuron, norea, and linuron were promising to the okra crop.

  6. (Abelmoschus esculentus (L.) Moench)

    Kundapura V. Ravishankar

    as okra, is one of the important pod vegetable crops in tropics and subtropics .... study. Genotypes/accession number. 1. Kashi Vibhuti. 2. Komal 2486. 3. Kashi Lalima. 4 ..... the microsatellite-enriched library method (Phuekvilai and. Wolff 2013 ...

  7. Alginate-okra gum blend beads of diclofenac sodium from aqueous template using ZnSO4 as a cross-linker.

    Sinha, Priyanka; Ubaidulla, U; Hasnain, M Saquib; Nayak, Amit Kumar; Rama, Bobba

    2015-08-01

    Zinc (Zn(2+))-ion induced diclofenac sodium (DS)-loaded alginate-okra (Hibiscus esculentus) gum (OG) blend beads was successfully formulated through Zn(2+)-ion induced ionic-gelation cross-linking method in a complete aqueous environment. Effects of polymer-blend ratio and cross-linker concentration on drug encapsulation efficiency (DEE) and cumulative drug release at 8 h (R8h) were optimized by 3(2)-factorial design. The optimized formulation of Zn(2+)-ion induced DS-loaded alginate-OG beads demonstrated 89.27±3.58% of DEE and 43.73±2.83% of R8h. The bead sizes were within 1.10±0.07 to 1.38±0.14 mm. The bead surface morphology was analyzed by SEM. The drug-polymer interaction in the optimized bead matrix was analyzed by FTIR and P-XRD. These beads exhibited sustained in vitro drug release over a prolonged period of 8h and followed controlled-release (zero-order) pattern with super case-II transport mechanism. The swelling and degradation of the optimized beads was influenced by the pH of test mediums, which might be suitable for intestinal drug delivery. Copyright © 2015 Elsevier B.V. All rights reserved.

  8. Kinetic thermal degradation of vitamin C during microwave drying of okra and spinach.

    Dadali, Gökçe; Ozbek, Belma

    2009-01-01

    In this present study, the effect of microwave output power and sample amount on vitamin C loss in okra (Hibiscus esculenta L.) and spinach (Spinacia oleracea L.) were investigated using the microwave drying technique. The procedure is based on the reaction between l-ascorbic acid (vitamin C) and 2,6-dichloroindophenol. The proposed method was applied successfully to both okra and spinach for the determination of ascorbic acid (vitamin C) content. It was observed that as the microwave output power increased or as the sample amount decreased, the vitamin C in okra and spinach decreased as well. The activation energy for degradation of vitamin C for both okra and spinach was calculated using an exponential expression based on the Arrhenius equation.

  9. De Novo Transcriptome Assembly and Characterization of the Synthesis Genes of Bioactive Constituents in Abelmoschus esculentus (L.) Moench

    Zhang, Chenghao; Dong, Wenqi; Gen, Wei; Xu, Baoyu; Shen, Chenjia

    2018-01-01

    Abelmoschus esculentus (okra or lady’s fingers) is a vegetable with high nutritional value, as well as having certain medicinal effects. It is widely used as food, in the food industry, and in herbal medicinal products, but also as an ornamental, in animal feed, and in other commercial sectors. Okra is rich in bioactive compounds, such as flavonoids, polysaccharides, polyphenols, caffeine, and pectin. In the present study, the concentrations of total flavonoids and polysaccharides in five organs of okra were determined and compared. Transcriptome sequencing was used to explore the biosynthesis pathways associated with the active constituents in okra. Transcriptome sequencing of five organs (roots, stem, leaves, flowers, and fruits) of okra enabled us to obtain 293,971 unigenes, of which 232,490 were annotated. Unigenes related to the enzymes involved in the flavonoid biosynthetic pathway or in fructose and mannose metabolism were identified, based on Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. All of the transcriptional datasets were uploaded to Sequence Read Archive (SRA). In summary, our comprehensive analysis provides important information at the molecular level about the flavonoid and polysaccharide biosynthesis pathways in okra. PMID:29495525

  10. Hybridization studies in Okra (Abelmoschus spp (L.) Moench)

    Amitaaba, Theophilus

    2016-07-01

    Okra (Abelmoschus spp. L. Moench) is an important multi-purpose vegetable crop cultivated and consumed across all tropical and temperate regions of the world. In Ghana, it is popular in all ten regions and increasing quantities are exported to Europe in the fresh form. The crop has received little attention by way of breeding to produce varieties combining the most desirable qualities to boost local cultivation and export. Ten accessions of Abelmoschus spp., comprising two species, A. esculentus (T1, T2, T3, VT, ID and AG) and A. callei (KB, AM, YL and T4) collected from six geographical regions of Ghana were crossed in all possible combinations to assess inter-specific as well as intra-specific hybridisation between and within species. Reciprocal crosses were also carried out and the performances of their F1 offspring were evaluated against the respective parents for expression of heterosis for key quantitative traits including days to 50% germination, days to 50% flowering, plant height, fresh fruit weight, length of pod and number of seeds per pod. Genetic relatedness among the accessions and their progeny was established by way of a dendrogrom based on furthest neighbour method (Euclidean). All six accessions of Abelmoschus esculentus were able to hybridize with one another in both direct and reciprocal cross combinations with high degree of crossability index (CI) (45.71% to 90.32%). On the other hand, cross-compatibility among A. esculentus and A. callei was successful only in one direction when A. esculentus was used asesculentus was used as females also with a CI between 34.48% and 60%. Parental lines T3 and T1 emerged as the most compatible female and male respectively. Crossability success was relatively high during early hours of the day but decreased continuously in subsequent hours. Ten parental accessions and 61 F1 progenies of A. esculentus and A.callei evaluated for 15 qualitative and 8 quantitative traits exhibited significant variations in all

  11. TOLERANCE OF Abelmoschus esculentus (L

    Cletus

    Key word: - Tolerance, diesel oil, polluted soil, Abelmoschus esculentus. INTRODUCTION ... errors -of the mean values were calculated for the replicate readings and data .... African Schools and Colleges, 2nd Ed. University Press Limited ...

  12. TIGER NUT (CYPERUS ESCULENTUS): COMPOSITION ...

    DR. AMINU

    This paper is a review on little history and the composition of Tigernut ranging from proximate, mineral and amino ..... thought to be beneficial to diabetics and those seeking to reduce ... (Cyperus esculentus) souced from a market in Ogbomoso ...

  13. Glycinebetaine-induced modulation in some biochemical and physiological attributes of okra under salt

    Saeed, H.M.; Mirza, J.I.

    2016-01-01

    Role of glycinebetaine (GB) in okra (Abelmoschus esculentus L. Moench) cv. Subz-pari plants grown under salinity stress was investigated under field conditions. The crop was planted under varying levels (0, 200 and 400 mg NaCl per kg of soil) of salinity stress. Foliar application of 75 mM GB was employed at two phases i.e. after 30 and 60 days of sowing. Imposition of salinity stress significantly increased leaf GB and proline contents but significantly reduced leaf chlorophyll content and physiological characteristics such as rate of photosynthesis (Pn), rate of transpiration (E), stomatal conductance (gs) and leaf relative water content (LRWC). Exogenous application of GB significantly increased GB content but decreased proline content of leaves and improved various gas exchange characteristics/physiological parameters. The present results thus indicated that foliar application of GB (75 mM) can modulate various biochemical and gas exchange parameters of okra, grown under salt stress. (author)

  14. Flavonoids derived from Abelmoschus esculentus attenuatesUV-B Induced cell damage in human dermal fibroblasts throughNrf2-ARE pathway

    Juilee Patwardhan; Purvi Bhatt

    2016-01-01

    Background: Ultraviolet-B (UV-B) radiation is a smaller fraction of the total radiation reaching the Earth but leads to extensive damage to the deoxyribonucleic acid (DNA) and other biomolecules through formation of free radicals altering redox homeostasis of the cell. Abelmoschus esculentus (okra) has been known in Ayurveda as antidiabetic, hypolipidemic, demulscent, antispasmodic, diuretic, purgative, etc. Objective: The aim of this study is to evaluate the protective effect of flavonoids f...

  15. Summer cover crops and soil amendments to improve growth and nutrient uptake of okra

    Wang, Q.R.; Li, Y.C.; Klassen, W. [University of Florida, Homestead, FL (United States). Center for Tropical Research & Education

    2006-04-15

    A pot experiment with summer cover crops and soil amendments was conducted in two consecutive years to elucidate the effects of these cover crops and soil amendments on 'Clemson Spineless 80' okra (Abelmoschus esculentus) yields and biomass production, and the uptake and distribution of soil nutrients and trace elements. The cover crops were sunn hemp (Crotalaria juncea), cowpea (Vigna unguiculata), velvetbean (Mucuna deeringiana), and sorghum sudan-grass (Sorghum bicolor x S. bicolor var. sudanense) with fallow as the control. The organic soil amendments were biosolids (sediment from wastewater plants), N-Viro Soil (a mixture of biosolids and coal ash), coal ash (a combustion by-product from power plants), co-compost (a mixture of 3 biosolids: 7 yard waste), and yard waste compost (mainly from leaves and branches of trees and shrubs, and grass clippings) with a soil-incorporated cover crop as the control. As a subsequent vegetable crop, okra was grown after the cover crops, alone or together with the organic soil amendments, had been incorporated. All of the cover crops, except sorghum sudangrass in 2002-03, significantly improved okra fruit yields and the total biomass production. Both cover crops and soil amendments can substantially improve nutrient uptake and distribution. The results suggest that cover crops and appropriate amounts of soil amendments can be used to improve soil fertility and okra yield without adverse environmental effects or risk of contamination of the fruit. Further field studies will be required to confirm these findings.

  16. Phytochemical analysis, antioxidant, antistress, and nootropic activities of aqueous and methanolic seed extracts of ladies finger (Abelmoschus esculentus L.) in mice.

    Doreddula, Sathish Kumar; Bonam, Srinivasa Reddy; Gaddam, Durga Prasad; Desu, Brahma Srinivasa Rao; Ramarao, Nadendla; Pandy, Vijayapandi

    2014-01-01

    Abelmoschus esculentus L. (ladies finger, okra) is a well-known tropical vegetable, widely planted from Africa to Asia and from South Europe to America. In the present study, we investigated the in vitro antioxidant capacity and in vivo protective effect of the aqueous and methanolic seed extracts of Abelmoschus esculentus against scopolamine-induced cognitive impairment using passive avoidance task and acute restraining stress-induced behavioural and biochemical changes using elevated plus maze (EPM) and forced swimming test (FST) in mice. Our results demonstrated that the pretreatment of mice with aqueous and methanolic seed extracts of Abelmoschus esculentus (200 mg/kg, p.o.) for seven days significantly (P nootropic activities which promisingly support the medicinal values of ladies finger as a vegetable.

  17. Evaluation of Hibiscus esculentus Linn. on the Mechanical and ...

    Nx 6110

    of lamination and capping are of particular concern. REFERENCES. [1]. J.N. BeMiller, R.L. Whistler, D.G.. Barkalow and C.C. Chen in R. L.. Whistler and J. N. BeMiller (eds.). Industrial Gums, 3rd Edition, Academic. Press, New York. 1993, p 227-256. [2]. R.C. Woolfall, Soap Perfume Cosmet. 37 (1964) 965-970. [3]. O.A. Itiola ...

  18. Evaluation of Hibiscus esculentus Linn. on the Mechanical and ...

    Malvaceae) on the mechanical and disintegration properties of paracetamol tablets were investigated against gum acacia as a standard binding agent. The effects of the nature and concentration of the mucilage binder and the relative density ...

  19. Hibiscus sabdari

    AISA

    Roselle (Hibiscus sabdariffa L.) is a malvaceae cultivated mainly for food and manufacture of textile fibers. ... confection d'un jus délicieux appelé « bissap » ... sorgho. La production africaine ne représente qu'environ 2 % de la production mondiale de fibres textiles. Les travaux conduits sur cette plante en Afrique ont ...

  20. Analysis of factors influencing adoption of okra production ...

    Socio-economic factors influencing adoption of okra technology packages in Enugu State, Nigeria was studied and analyzed in 2012. Purposive and multistage random sampling techniques were used in selecting communities, villages and Okra farmers. The sample size was 90 okra farmers (45Awgu and 45 Aninri Okra ...

  1. Sortiment Hibiscus in beweging

    Hoffman, M.H.A.

    2010-01-01

    Hibiscus is hot. Op Plantarium 2009 werden maar liefst acht nieuwe cultivars ter keuring aangeboden. Bovendien winnen de laatste jaren ook andere soorten dan de bekende Hibiscus syriacus aan populariteit

  2. Effect of variety on the storage of Nigeria indigenous okra ...

    , condensed tannin, total phenol, vitamin C and reducing power of the Okra were determined on alternate days. 'Lokoja' okra pods experienced the least percentage loss in all the determined parameters. At the end of the storage period, pods ...

  3. 7 CFR 319.56-21 - Okra from certain countries.

    2010-01-01

    ... Okra from certain countries. Okra from Brazil, Colombia, Ecuador, Guyana, Mexico, Peru, Suriname, Venezuela, and the West Indies may be imported into the United States in accordance with this section and...

  4. WATER AND PHYSIOLOGICAL RESPONSES OF OKRA ...

    H. Rahim Guealia

    1 sept. 2017 ... for agricultural production. ... bentonite (WB) under controlled greenhouse conditions. ..... esculentus L.) âgées de deux mois cultivé sur substrat sans ..... Kırnak H et Taş I. Amelioratrive effect of calcium nitrate on cucumber.

  5. Flavonoids Derived from Abelmoschus esculentus Attenuates UV-B Induced Cell Damage in Human Dermal Fibroblasts Through Nrf2-ARE Pathway.

    Patwardhan, Juilee; Bhatt, Purvi

    2016-05-01

    Ultraviolet-B (UV-B) radiation is a smaller fraction of the total radiation reaching the Earth but leads to extensive damage to the deoxyribonucleic acid (DNA) and other biomolecules through formation of free radicals altering redox homeostasis of the cell. Abelmoschus esculentus (okra) has been known in Ayurveda as antidiabetic, hypolipidemic, demulscent, antispasmodic, diuretic, purgative, etc. The aim of this study is to evaluate the protective effect of flavonoids from A. esculentus against UV-B-induced cell damage in human dermal fibroblasts. UV-B protective activity of ethyl acetate (EA) fraction of okra was studied against UV-B-induced cytotoxicity, antioxidant regulation, oxidative DNA damage, intracellular reactive oxygen species (ROS) generation, apoptotic morphological changes, and regulation of heme oxygenase-1 (HO-1) gene through nuclear factor E2-related factor 2-antioxidant response element (Nrf2-ARE) pathway. Flavonoid-rich EA fraction depicted a significant antioxidant potential also showing presence of rutin. Pretreatment of cells with EA fraction (10-30 μg/ml) prevented UV-B-induced cytotoxicity, depletion of endogenous enzymatic antioxidants, oxidative DNA damage, intracellular ROS production, apoptotic changes, and overexpression of Nrf2 and HO-1. Our study demonstrated for the 1(st) time that EA fraction of okra may reduce oxidative stress through Nrf2-ARE pathway as well as through endogenous enzymatic antioxidant system. These results suggested that flavonoids from okra may be considered as potential UV-B protective agents and may also be formulated into herbal sunscreen for topical application. Flavonoid-enriched ethyl acetate (EA) fraction from A. esculentus protected against ultraviolet-B (UV-B)-induced oxidative DNA damageEA fraction prevented UV-B-induced cytotoxicity, depletion of endogenous enzymatic antioxidants, and intracellular reactive oxygen species productionEA fraction could reduce oxidative stress through the Nrf2-ARE

  6. Soil amendment with halophytes induces physiological changes and reduces root-knot infection in eggplant and okra

    Waseem M. ABBASI

    2011-01-01

    Full Text Available Root-knot nematode, Meloidogyne javanica (Treub Chitwood is a soil-borne plant pathogen of roots. Nematode infection results in altered plant growth and physicochemical processes due to gall formation. Many plants contain unique biochemicals that have biocidal properties and offer a potential novel approach to suppress the nematode populations in soil and improve growth of crop plants. In the present study effect of some indigenous halophytic plant species (Tamarix indica Willd, Suaeda fruticosa Forssk and Salsola imbricata (Schultz Dandy were tested against M. javanica. Tested halophytes significantly (P<0.001 reduced egg hatching and caused mortality of second stage juveniles (J2 in vitro. These halophytes when incorporated in soil (0.3, 0.5 and 1% w/w markedly increased growth of eggplant (Solanum melongena L. cv. Black beauty and okra (Abelmoschus esculentus [L.] Moench. cv. Arka anamika and provided control of root-knot infection at higher doses (0.5 and 1%. Amended eggplants and okra showed significant (P<0.001 increase in chlorophylls and decrease in chlorophyll a/b ratio. Protein concentration in leaves of both the plants were increased with 1% amendment of S. fruticosa and S. imbricata. While nucleic acid concentrations were varied with different treatments.  

  7. (Psidium guajava), hibiscus (Hibiscus-rosa sinensis)

    SAM

    2014-04-30

    Hibiscus-rosa ... properly before consumption to avoid ingestion of possible food pathogens that cause food-borne ... depending on the one that most supports their growth ..... boundary leaf, these compounds which include CO2,.

  8. Evaluation of two okra species [Abelmoschusesculentus (L ...

    DizMat_Ogwu

    2014-02-26

    Feb 26, 2014 ... African Journal of Biotechnology. Full Length Research Paper. Evaluation of two okra species ..... Amakiri JO, Onofeghara FA (1984). Effect of crude oil pollution on the growth of Zea mays, Abelmoschusesculentusand capsicum frutescans. Oil Pet. Pollut. 1:199-206. Ariyo OJ (1993). Genetic diversity in west ...

  9. Hibiscus tiliaceus L

    James A. Allen

    2002-01-01

    The genus Hibiscus includes about 200 species distributed primarily in the tropical and subtropical regions of the world. It is a highly variable genus, with relatively few characteristics in common (Wagner and others 1990). Hibiscus tiliaceus is one of the most widespread species in the genus, with a pantropical, mainly...

  10. Hibiscus acetosella 'Sahara Sunset'

    The Agricultural Research Service, United States Department of Agriculture, hereby release to nurserymen a new African Hibiscus, Hibiscus acetosella Welw. ex Hiern., named ‘Sahara Sunset’. This cultivar, tested as HAC06-11 was selected from a group seedlings grown at the Thad Cochran Southern Horti...

  11. genotypes for okra yellow vein mosaic virus incidence

    SAM

    2014-03-19

    Mar 19, 2014 ... by whitefly (Bemisia tabaci Gen.) (Rana et al., 2006). ... Solankey et al. 1337. Table 1. Scale for classifying disease reaction in okra to OYVMV disease. Symptom. Severity grade. Response value. Coefficient of infection. Reaction ..... On the basis of two season research work, it was observed that the okra ...

  12. Microflora diversity on the phyloplane of wild Okra ( Corchorus ...

    Bacterial genera isolated included; Staphylococcus, Bacillus, Micrococcus, Serratia and Proteus. Rhodotorula, Mucor, Trichoderma, Aspergillus, Penicillium, and Helminthosporium were the genera of fungi isolated. Further studies could help to elucidate major players in wild okra phylloplane ecology. Keywords: Wild Okra ...

  13. Taxonomic evaluation using pollen grain sculpture and seed coat characters of 11 taxa of genus Hibiscus (Malvaceae in Egypt

    M.A. El-Kholy

    2011-06-01

    Full Text Available Pollen grain morphology and seed coat characters of 11 cultivars belonging to two species of genus Hibiscus (Family Malvaceae namely H. esculentus, H. abelmoschus and H. sabdariffa were investigated. This study was carried out using light microscope (LM and scanning electron microscopy (SEM. Pollen morphology of this genus is fairly uniform. Generally radially symmetrical apolar, mostly spheroidal, pantoporate. Seed exomorphic characters revealed four types of ornamentations; reticulate, ocealate, foveolate and ruminate. Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS–PAGE was employed to characterize those taxa. Thirty-one bands of seed protein profiles have been constructed from the gel. The produced dendrograms that were analyzed by STATISCA program using UPGMA clustering method showed a close affinity among the seven H. esculentus cultivars and the four H. sabdariffa cultivars.

  14. Acetylated Rhamnogalacturonans from Immature Fruits of Abelmoschus esculentus Inhibit the Adhesion of Helicobacter pylori to Human Gastric Cells by Interaction with Outer Membrane Proteins

    Christian Thöle

    2015-09-01

    Full Text Available Polysaccharide containing extracts from immature fruits of okra (Abelmoschus esculentus are known to exhibit antiadhesive effects against bacterial adhesion of Helicobacter pylori (H. pylori to stomach tissue. The present study investigates structural and functional features of polymers responsible for this inhibition of bacterial attachment to host cells. Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates after preincubation of the bacteria at 1 mg/mL. Sequential extraction of okra fruits yielded hot buffer soluble solids (HBSS with dose dependent antiadhesive effects against strain J99 and three clinical isolates. Preincubation of H. pylori with HBSS (1 mg/mL led to reduced binding to 3ʹ-sialyl lactose, sialylated Lea and Lex. A reduction of bacterial binding to ligands complementary to BabA and SabA was observed when bacteria were pretreated with FE90%. Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains. Deacetylation of HBSS and FE90% resulted in loss of the antiadhesive activity, indicating esterification being a prerequisite for antiadhesive activity.

  15. Whitefly population dynamics in okra plantations Dinâmica populacional de mosca-branca em quiabo

    Germano Leão Demolin Leite

    2005-01-01

    Full Text Available The control of whitefly Bemisia tabaci (Gennadius biotype B (Hemiptera: Aleyrodidae on okra (Abelmoschus esculentus L. consists primarily in the use of insecticides, due to the lack of information on other mortality factors. The objective of this study was to evaluate the spatial and temporal population dynamics of the whitefly B. tabaci biotype B on two successive A. esculentus var. "Santa Cruz" plantations. Leaf chemical composition, leaf nitrogen and potassium contents, trichome density, canopy height, plant age, predators, parasitoids, total rainfall and median temperature were evaluated and their relationships with whitefly on okra were determined. Monthly number estimates of whitefly adults, nymphs (visual inspection and eggs (magnifying lens occurred on bottom, middle and apical parts of 30 plants/plantation (one leaf/plant. Plants senescence and natural enemies, mainly Encarsia sp., Chrysoperla spp. and Coccinellidae, were some of the factors that most contributed to whitefly reduction. The second okra plantation, 50 m apart from the first, was strongly attacked by whitefly, probably because of the insect migration from the first to the second plantation. No significant effects of the plant canopy on whitefly eggs and adults distribution were found. A higher number of whitefly nymphs was found on the medium part than on the bottom part.O controle da mosca-branca Bemisia tabaci (Gennadius biótipo B (Hemiptera: Aleyrodidae em quiabeiro (Abelmoschus esculentus L. consiste principalmente no uso de inseticidas, em virtude da falta de informação sobre outros fatores de mortalidade. O objetivo deste trabalho foi compreender a dinâmica populacional, espacial e temporal da mosca-branca em dois cultivos sucessivos de quiabeiro "Santa Cruz". Avaliaram-se a composição química foliar, os níveis foliares de nitrogênio e de potássio, a densidade de tricomas, a altura de dossel, a idade de planta, predadores, parasitóides, pluviosidade total

  16. NMR study of Albemoschus esculentus characterization

    Bathista, A.L.B.S; Silva, E.O.; Nogueira, Jose de S.; Tavares, M.I.B.

    2001-01-01

    The investigation of the main compounds presented in the Albemoschus esculentus has been carried out employing nuclear magnetic resonance spectroscopy (NMR), using solution and solid state NMR when it one was necessary. The evaluation of NMR data allowed us to characterize the main type of components presented in this kind of sample. It was necessary to use a total information from solid state NMR and also the solution response. From these information we could get that four main components were presented in this sample. One in the shell, that is cellulose, another one between the shell and seeds that is a polysaccharide and in the seed two components were found one is a starch and the second one is an oil, a triacylglycerol. These components are responsible by its physical chemistry properties. (author)

  17. Genetic relationships among West African okra ( Abelmoschus caillei )

    Abelmoschus caillei) and 43 Asian genotypes (A. esculentus) were assessed for genetic distinctiveness and relationships using random amplified polymorphic DNA (RAPD). The molecular analysis showed that all the thirteen primers used ...

  18. Assessment of imidacloprid and acetamiprid residues in okra and eggplant grown in peri-urban areas and their dietary intake in humans

    Amjad, A.; Akhtar, S.; Randhawa, M.; Binyameen, M.

    2018-01-01

    This research work was designed to estimate the gradually increasing use of insecticides on vegetables to create awareness among the consumers regarding their insecticide residues. The main aim of this study is to highlight the harmful effects of insecticides in comparison with dietary intake assessment of vegetables. In this regard, total 180 (n = 60×3) samples of okra (Abelmoschus esculentus) and eggplant (Solanum melongena) comprising of 30 samples vegetable were collected from the peri-urban farming system of Multan, Faisalabad and Gujranwala to assess insecticide residues along with their dietary intake assessment. The selected samples were quantified for the insecticide residues by using High Performance Liquid Chromatography (HPLC) technique equipped with UV/Visible detector. Results revealed that imidacloprid residues found in eggplant (0.226 mg Kg-1) and okra (0.176 mg Kg-1) from Multan region were greater than the residues reported from Gujranwala and Faisalabad. Out of all analyzed samples for imidacloprid and acetamaprid residues, 58% and 65% samples contained detectable residues respectively. Whereas 10% and 15% samples exceeded their maximum residue limits (MRLs) established by European Commission. Dietary intake assessment for imidacloprid and acetamiprid was calculated as per their maximum permissible intake (MPI) values i.e. 3.84 and 4.48 mg person-1day-1, respectively, which revealed that although a reasonable proportion of samples exceeded MRLs but their consumption was found within safe limit. (author)

  19. Combined effects of Psoralens and ultraviolet-B on growth, pigmentation and biochemical parameters of Abelmoschus esculentus L.

    Kumari, Rima; Singh, Suruchi; Agrawal, S B

    2009-05-01

    The effects of pre-treatment of Psoralens (furocoumarin compounds) and supplemental ultraviolet-B (sUV-B) were studied on plant growth, photosynthetic and non-photosynthetic pigments, protein, phenylalanine ammonia lyase (PAL) activity and antioxidative defense potential as well as their ultimate effects on biomass production in Abelmoschus esculentus L. (Okra) plants. Psoralens are capable of absorbing radiant energy and stimulating the pigmentation of human skin when photo-activated in presence of UV-A or UV-B making them beneficial in the treatment of vitilago. Pre-treatment of Psoralens against sUV-B (pUV-B), stimulates higher production of UV-B protective pigments (flavonoids and carotenoids) and helps in maintaining its biomass against UV-B stress. Antioxidative defense system in the test plant was activated by combined treatment of Psoralens and sUV-B as evidenced by the enhanced activity of enzymatic (ascorbate peroxidase-APX, superoxide dismutase-SOD, POX) and non-enzymatic (ascorbic acid and phenol) antioxidants. Individual treatments of Psoralens and sUV-B showed inhibitory effect on various morphological traits i.e. reduction in plant height, leaf area and ultimately on biomass production. Our results clearly indicated that adverse effect of sUV-B on biomass production was ameliorated by pre- treatment with Psoralens.

  20. Reduce pests, enhance production: benefits of intercropping at high densities for okra farmers in Cameroon.

    Singh, Akanksha; Weisser, Wolfgang W; Hanna, Rachid; Houmgny, Raissa; Zytynska, Sharon E

    2017-10-01

    Intercropping can help reduce insect pest populations. However, the results of intercropping can be pest- and crop-species specific, with varying effects on crop yield, and pest suppression success. In Cameroon, okra vegetable is often grown in intercropped fields and sown with large distances between planting rows (∼ 2 m). Dominant okra pests include cotton aphids, leaf beetles and whiteflies. In a field experiment, we intercropped okra with maize and bean in different combinations (okra monoculture, okra-bean, okra-maize and okra-bean-maize) and altered plant densities (high and low) to test for the effects of diversity, crop identity and planting distances on okra pests, their predators and yield. We found crop identity and plant density, but not crop diversity to influence okra pests, their predators and okra yield. Only leaf beetles decreased okra yield and their abundance reduced at high plant density. Overall, okra grown with bean at high density was the most economically profitable combination. We suggest that when okra is grown at higher densities, legumes (e.g. beans) should be included as an additional crop. Intercropping with a leguminous crop can enhance nitrogen in the soil, benefiting other crops, while also being harvested and sold at market for additional profit. Manipulating planting distances and selecting plants based on their beneficial traits may thus help to eliminate yield gaps in sustainable agriculture. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  1. Verbeter Hibiscus met voeding

    Aendekerk, T.G.L.

    2003-01-01

    Onderzoek naar de invloed van pH en de voedingsoplossing op groeiafwijkingen bij Hibiscus. Gegevens in bijgaande tabellen: 1) Gebruikte voedingsoplossingen (waarden in mmol/l); 2) Beoordeling bij gebruikte voedingsoplossingen; 3) Optimale voedingsoplossingen (waarden in mmol/l) in twee periodes; 4)

  2. Antiproliferative and proapoptotic actions of okra pectin on B16F10 melanoma cells

    Vayssade, M.; Sengkhamparn, N.; Verhoef, R.P.; Delaigue, C.; Goundiam, O.; Vigneron, P.; Voragen, A.G.J.; Schols, H.A.; Nagel, M.D.

    2010-01-01

    The proliferation and apoptosis of metastatic melanoma cells are often abnormal. We have evaluated the action of a pectic rhamnogalacturonan obtained by hot buffer extraction of okra pods (okra RG-I) on melanoma cell growth and survival in vitro. We added okra RG-I containing an almost pure RG-I

  3. Tien jaar Knolcyperus (Cyperus esculentus L.) in Nederland

    Rotteveel, A.J.W.

    1993-01-01

    Yellow nutsedge ( Cyperus esculentus L.) was first recorded in 1981 as an agricultural pest. A considerable number of fields was found to be infested. Because the species is very damaging to agriculture legal measures were taken to restrict the dispersion and promote the control of the weed. During

  4. A baseline survey of tiger nut ( Cyperus esculentus ) production in ...

    Tiger nut (Cyperus esculentus) is a minor but important crop in Ghana. In a survey conducted on the production and marketing of the crop at Aduamoa in the Kwahu South District of Ghana, it was observed that tiger nut production was predominantly the work of women, with 70 per cent of farmers being women and 30 per ...

  5. Genomic Sequencing of Ranaviruses Isolated from Edible Frogs (Pelophylax esculentus)

    Ariel, Ellen; Subramaniam, Kuttichantran; Imnoi, Kamonchai

    2017-01-01

    Ranaviruses were isolated from wild edible frogs (Pelophylax esculentus) during epizootics in Denmark and Italy. Phylogenomic analyses revealed that these isolates are closely related and belong to a clade of ranaviruses that includes the Andrias davidianus ranavirus (ADRV), common midwife toad r...

  6. Okra yield fertilized with bovine manure and biofertilizer

    Ademar Pereira de Oliveira

    2013-12-01

    Full Text Available The use of bovine manure becomes an useful and economic practice for the small and medium producers of vegetables, and the okra plant normally demands high doses of organic fertilizers. This study was carried out, from January to July 2011, at the Federal University of Paraíba, in Areia city - PB, aiming to evaluate the effect of bovine manure and biofertilizer on the productive behavior of the okra plant. The experimental design used was randomized blocks, with four repetitions in factorial scheme 6 x 2, with the doses factors of bovine manure (0, 10, 20, 30, 40 and 50 t ha-1 with and without biofertilizer. The average mass of commercial fruit of okra, with and without biofertilizer was 18 and 16.5 g, respectively, in the doses of 27.5 and 60 t ha-1 of manure. The number of fruit plant-1 without biofertilizer was 30 fruits plant-1 of okra in the dose of 60 t ha-1 and with biofertilizer, the number of fruits plant-1 was 33 fruits in the dose of 28 t ha-1 of bovine manure. The productivity of commercial fruits of okra without biofertilizer was 20.4 t ha-1 and 22 t ha-1 with biofertilizer, respectively, in the doses of 60 and 31 t ha-1 of bovine manure.

  7. Growth Responses of Two Cultivated Okra Species (Abelmoschus ...

    Abelmoschus esculentus was investigated using six accessions; three for each species in crude oil contaminated soil. The seeds ..... industrial waste. Environmental and Experimental. Botany, 52.79-88. Siemonsma, J. S and Hamon, S. (2002). Abelmoschus caillei (A. chev) Stevels. In: Oyen, L.P.A. and. Lemmens R.H.M. ...

  8. Nutritional response of Okra to various packaging materials and ...

    The research is focused at determining the nutritional response of Okra when pickling is carried out using different antimicrobial liquids (vinegar, olive oil, and groundnut oil) and packaging materials (glass jar, plastic and stainless steel), as well as make a comparism when spices (garlic, ginger, mixture of garlic and ginger) ...

  9. design, construction and evaluation of motorized okra slicer

    Dr Obe

    +Federal Institute of Industrial Research. Oshodi Lagos. *Department of Agricultural and Bioresources ... manually operated machine and hand slicing methods. It produces slices of uniform thickness with standard .... feeding okra into the machine and finally weighing all the slices irrespective of damage. The damaged ...

  10. 77 FR 6772 - United States Standards for Grades of Okra

    2012-02-09

    ... DEPARTMENT OF AGRICULTURE Agricultural Marketing Service [Document No. AMS-FV-11-0054] United States Standards for Grades of Okra AGENCY: Agricultural Marketing Service, USDA. ACTION: Notice. SUMMARY: The Agricultural Marketing Service (AMS), prior to undertaking research and other work associated with...

  11. Morphological classification of genetic diversity in cultivated okra ...

    Administrator

    2011-09-19

    Sep 19, 2011 ... the pains and effects of haemorrhoid. ... under a good cropping system, with yield varying from .... Short Mouth Ibarapa ..... b e tw e e n clu ste rs. Figure 2. Dendrogram SLCA of the 29 okra accessions in the early ... to fertilizer.

  12. Studies on mutation breeding of hibiscus Syriacus

    Song, Heui Sub; Lee, Ki Woon; Im, Yong Taek [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1994-12-01

    Hibiscus(Hibiscus syracuse L.) has been know as a national flower of Korea science old times. Although there are some ancient records that the Hibiscus had been planted in large quantities in Korea, Japanese had dug out all the good plants of Hibiscus in this country during their colonial period. But Hibiscus has such a characteristics of self-incompatibility that all the plants exist as a hybrid naturally and have heterogeneous genes. Therefore many good characters can be taken out from the surviving plants. Many domestic 78 varieties of Hibiscus syracuse were collected and propagated 26 varieties cuttings. Radiosensitivity of gamma-ray irradiated Hibiscus syracuse were investigated the germination rate, survival rate, plant height was with the increase of 4 kR better than control. The radiation doses of 10-12 kR are recommended for mutation breeding of Hibiscus. 6 figs, 11 tabs, 41 refs. (Author).

  13. Studies on mutation breeding of hibiscus Syriacus

    Song, Heui Sub; Lee, Ki Woon; Im, Yong Taek

    1994-12-01

    Hibiscus(Hibiscus syracuse L.) has been know as a national flower of Korea science old times. Although there are some ancient records that the Hibiscus had been planted in large quantities in Korea, Japanese had dug out all the good plants of Hibiscus in this country during their colonial period. But Hibiscus has such a characteristics of self-incompatibility that all the plants exist as a hybrid naturally and have heterogeneous genes. Therefore many good characters can be taken out from the surviving plants. Many domestic 78 varieties of Hibiscus syracuse were collected and propagated 26 varieties cuttings. Radiosensitivity of gamma-ray irradiated Hibiscus syracuse were investigated the germination rate, survival rate, plant height was with the increase of 4 kR better than control. The radiation doses of 10-12 kR are recommended for mutation breeding of Hibiscus. 6 figs, 11 tabs, 41 refs. (Author)

  14. S Abelm okra l Screen mosch leaf cu field c ing of hus cal url and ...

    sunny

    y okra has a reas late okra. 1977; Nwoke. 1998). World uit-vegetable on License 4.0 y n or a .... nineties while the substantive work was carried out in the Department of Crop ... measuring 6.5 x 6.0 m for the protected plots and 7.5 x 7.0 m for ..... gen the oth spe the obv imp. 26 Afr. J igure 3. Chron. Okra Leaf curl a urling and ...

  15. Inheritance of okra leaf type in different genetic backgrounds and its ...

    use

    2011-11-21

    Nov 21, 2011 ... discontinuous variation for leaf shape in F2 generations of three crosses .... Variable classes in leaf types (a) Normal leaf (b) Okra leaf (c) Sub-okra leaf. ..... insect pests on different isogenic lines of cotton variety H-777. J.

  16. Effect of intercropping varieties of sweet potato and okra in an ultisol ...

    A field experiment was conducted at the research farm of the National Root Crops Research Institute (NRCRI), Umudike in 2000 and 2001 cropping seasons to access the productivity of three sweet potato cultivars intercropped with three okra cultivars. Intercropping generally increased okra plant height while intercropping ...

  17. Seed yield and quality of okra as influenced by sowing dates

    Administrator

    2011-06-20

    Jun 20, 2011 ... and quality seed of okra over four consecutive years having seven sowing times at fortnight interval ..... Figures in parentheses are angular transformed values. ... Table 6. Step-wise regression model on seed yield of okra with ...

  18. Crystal structures of hibiscus acid and hibiscus acid dimethyl ester isolated from Hibiscus sabdariffa (Malvaceae)

    Zheoat, Ahmed M.; Gray, Alexander I.; Igoli, John O.; Kennedy, Alan R.; Ferro, Valerie A.

    2017-01-01

    The biologically active title compounds have been isolated from Hibiscus sabdariffa plants, hibiscus acid as a dimethyl sulfoxide monosolvate [systematic name: (2S,3R)-3-hy?droxy-5-oxo-2,3,4,5-tetra?hydro?furan-2,3-di?carb?oxy?lic acid dimethyl sulfoxide monosolvate], C6H6O7?C2H6OS, (I), and hibiscus acid dimethyl ester [systematic name: dimethyl (2S,3R)-3-hy?droxy-5-oxo-2,3,4,5-tetra?hydro?furan-2,3-di?carboxyl?ate], C8H10O7, (II). Compound (I) forms a layered structure with alternating laye...

  19. Sortiment Hibiscus voortdurend in beweging

    Hoffman, M.H.A.

    2010-01-01

    Hibiscus is hot en volop in ontwikkeling. Op Plantarium 2009 werden maarliefst acht nieuwe cultivars ter keuring aangeboden. In naamlijsten van 2005 staan ruim 150 cultivars geregistreerd, terwijl dit er in de naamlijst van 1995 slechts 60 waren. Alle reden om op een rijtje te zetten wat er bekend

  20. Studies on mutant breeding of Hibiscus syriacus

    Song, Hi Sup; Kim, Jin Kyu; Lee, Ki Un; Kim, Young Taik.

    1997-01-01

    Hibiscus has been known as a national flower of Korea. Hibiscus has such a characteristic of self-incompatibility that all the plant exist as natural hybrids and have heterogeneous genes. Many domestic 91 varieties of Hibiscus syriacus were collected. Radiosensitivity of H. Syriacus irradiated with γ-ray was investigated in plant cuttings. The plant height was reduced by 45% in 5KR irradiated group, compared to control group. The radiation dose of 5KR could be recommended for mutation breeding of Hibiscus cuttings. Radiosensitivity of γ-ray irradiated Hibiscus seed were investigated. The germination rate, survival rate and plant height was better in the 4KR irradiation plot than control. The radiation dose of 10∼12KR are recommended for mutation breeding of Hibiscus. Promising mutant lines were selected form the varieties of Hwarang, Wolsan no. 176, Ilpyondansim, Emille, Hanol, Yongkwang, Saeyongkwang, Chungmu, Imjinhong, Arang, Hungdansim-1 and Hongdansim-2. (author). 66 refs., 16 tabs., 13 figs

  1. Studies on mutant breeding of Hibiscus syriacus

    Song, Hi Sup; Kim, Jin Kyu; Lee, Ki Un; Kim, Young Taik

    1997-01-01

    Hibiscus has been known as a national flower of Korea. Hibiscus has such a characteristic of self-incompatibility that all the plant exist as natural hybrids and have heterogeneous genes. Many domestic 91 varieties of Hibiscus syriacus were collected. Radiosensitivity of H. Syriacus irradiated with {gamma}-ray was investigated in plant cuttings. The plant height was reduced by 45% in 5KR irradiated group, compared to control group. The radiation dose of 5KR could be recommended for mutation breeding of Hibiscus cuttings. Radiosensitivity of {gamma}-ray irradiated Hibiscus seed were investigated. The germination rate, survival rate and plant height was better in the 4KR irradiation plot than control. The radiation dose of 10{approx}12KR are recommended for mutation breeding of Hibiscus. Promising mutant lines were selected form the varieties of Hwarang, Wolsan no. 176, Ilpyondansim, Emille, Hanol, Yongkwang, Saeyongkwang, Chungmu, Imjinhong, Arang, Hungdansim-1 and Hongdansim-2. (author). 66 refs., 16 tabs., 13 figs.

  2. Direct genetic transformation of Hibiscus sabdariffa L.

    Administrator

    After 60 days evaluation point, the assessment of the transformation by PCR revealed that H. sabdariffa line tested, carried the nptII gene. Key words: Hibiscus sabdariffa, genetic transformation. INTRODUCTION. Hibiscus sabdariffa is a crop widely cultivated in Sub. Saharan Africa, growing on sandy soils after the harvest.

  3. Microbial composition of guava (Psidium guajava), hibiscus ...

    Microbial composition of guava (Psidium guajava), hibiscus (Hibiscus-rosa sinensis), mango (Mangifera indica) and pumpkin (Telfairia occidentalis Hook) ... African Journal of Biotechnology ... The microbial genera isolated from this study showed that, both human and plant pathogens can colonize plants' phyllosphere.

  4. Investigating the characteristic strength of flocs formed from crude and purified Hibiscus extracts in water treatment.

    Jones, Alfred Ndahi; Bridgeman, John

    2016-10-15

    The growth, breakage and re-growth of flocs formed using crude and purified seed extracts of Okra (OK), Sabdariffa (SB) and Kenaf (KE) as coagulants and coagulant aids was assessed. The results showed floc size increased from 300 μm when aluminium sulphate (AS) was used as a coagulant to between 696 μm and 722 μm with the addition of 50 mg/l of OK, KE and SB crude samples as coagulant aids. Similarly, an increase in floc size was observed when each of the purified proteins was used as coagulant aid at doses of between 0.123 and 0.74 mg/l. The largest floc sizes of 741 μm, 460 μm and 571 μm were obtained with a 0.123 mg/l dose of purified Okra protein (POP), purified Sabdariffa (PSP) and purified Kenaf (PKP) respectively. Further coagulant aid addition from 0.123 to 0.74 mg/l resulted in a decrease in floc size and strength in POP and PSP. However, an increase in floc strength and reduced d50 size was observed in PKP at a dose of 0.74 mg/l. Flocs produced when using purified and crude extract samples as coagulant aids exhibited high recovery factors and strength. However, flocs exhibited greater recovery post-breakage when the extracts were used as a primary coagulant. It was observed that the combination of purified proteins and AS improved floc size, strength and recovery factors. Therefore, the applications of Hibiscus seeds in either crude or purified form increases floc growth, strength, recoverability and can also reduce the cost associated with the import of AS in developing countries. Crown Copyright © 2016. Published by Elsevier Ltd. All rights reserved.

  5. Chemical composition, nutritional value and antioxidant properties of Mediterranean okra genotypes in relation to harvest stage.

    Petropoulos, Spyridon; Fernandes, Ângela; Barros, Lillian; Ferreira, Isabel C F R

    2018-03-01

    The aim of the present study was to determine the effect of fruit size on nutritional value, chemical composition and antioxidant properties of Mediterranean okra genotypes. For this purpose, pods from four okra cultivars and local landraces commonly cultivated in Greece, as well as pods from four commercial cultivars from North America were collected at two sizes (3-5 and>7cm). Significant differences were observed between the studied genotypes for both nutritional value and chemical composition parameters. Small fruit had a higher nutritional value, whereas chemical composition differed in a genotype dependent manner with most of the studied cultivars showing better results when harvested in small size. In conclusion, fruit size has a genotype dependent impact on chemical composition and nutritional value of okra pods and the common practice of harvesting okra fruit while they still have a small size helps to increase nutritional value for most of the studied genotypes. Copyright © 2017 Elsevier Ltd. All rights reserved.

  6. Plant Growth-Promoting Rhizobacteria Enhance Salinity Stress Tolerance in Okra through ROS-Scavenging Enzymes

    Sheikh Hasna Habib

    2016-01-01

    Full Text Available Salinity is a major environmental stress that limits crop production worldwide. In this study, we characterized plant growth-promoting rhizobacteria (PGPR containing 1-aminocyclopropane-1-carboxylate (ACC deaminase and examined their effect on salinity stress tolerance in okra through the induction of ROS-scavenging enzyme activity. PGPR inoculated okra plants exhibited higher germination percentage, growth parameters, and chlorophyll content than control plants. Increased antioxidant enzyme activities (SOD, APX, and CAT and upregulation of ROS pathway genes (CAT, APX, GR, and DHAR were observed in PGPR inoculated okra plants under salinity stress. With some exceptions, inoculation with Enterobacter sp. UPMR18 had a significant influence on all tested parameters under salt stress, as compared to other treatments. Thus, the ACC deaminase-containing PGPR isolate Enterobacter sp. UPMR18 could be an effective bioresource for enhancing salt tolerance and growth of okra plants under salinity stress.

  7. Utilization of Degraded Chitosan for Growth Promoter and Blossom Blight Disease Controls in Okra

    Kewsuwan, Prartana

    2010-01-01

    The experiment focus on the comparison of the effect of gamma radiation and chemical reagent on degradation of chitosan and preliminary test of degraded chitosan with different molecular weight as plant growth promoter of okra in potting experiment. (author)

  8. Inheritance of okra leaf type in different genetic backgrounds and its ...

    use

    2011-11-21

    Nov 21, 2011 ... trait was studied in three cross combinations involving a common okra leaf parent ... pests with reduced leaf area allowing better air flow and maximum sunlight .... Irrigation both by canal and turbine water was applied to the.

  9. Use of the crop maize to reduce yellow nutsedge (Cyperus esculentus L. pressure in highly infested fields in Switzerland

    Keller, Martina

    2014-02-01

    Full Text Available Cyperus esculentus L. causes high yield losses in many crops worldwide. In Switzerland it was observed for the first time about 30 years ago. Since then it has become a serious weed in several regions - especially where vegetables are produced. Growing vegetables in heavily infested fields should be abandoned due to their low competitiveness and the lack of available, effective herbicides. Contrarily, in maize several herbicides with a partial effect on C. esculentus are registered. Thus, continuous cultivation of maize including the use of the most effective herbicides against C. esculentus could help reducing infestation levels in heavily infested fields. Field trials were carried out at two sites in maize during 2011 and 2012. Different herbicide combinations, hoeing and herbicide applications combined with hoeing were the applied treatments. Split application was compared with one single application of the same amount of product. The effect of an additional, late under leaf herbicide application was determined as well. Cyperus esculentus coverage was estimated in the fields in 2011. Plots were sampled in spring 2011, autumn 2011 and autumn 2012. The Number of C. esculentus sprouts germinated from the soil samples was determined under greenhouse conditions. The most effective herbicide combination of registered active ingredients was rimsulfuron and mesotrione. Smetolachlor was effective as well, especially if combined with mechanical weed control. Halosulfuron-methyl applied twice provided the best C. esculentus control. Split application tended to be more effective than a single application. Control of C. esculentus could be improved considerably with an additional herbicide application in late June (under leaf. The treatments with highest C. esculentus control determined in the field trials and combinations thereof are effective treatment options for C. esculentus control in maize. These findings indicate and confirm that maize cropped for

  10. Effect of pig manure on nutrient composition, growth and yield of Okra

    Five rates of manure, 0,2.5,5.0,7.5, and 10.0t/ha and NPK (15-15-15) fertilizer } at 200kg/ha were compared as to their effects on leaf nutrient composition, growth and pod yield of okra. Pig manure increased okra leaf nutrient N,P,K Ca and Mg contents, and the nutrients increased with application of manure. NPK fertilizer ...

  11. Crystal structures of hibiscus acid and hibiscus acid dimethyl ester isolated from Hibiscus sabdariffa (Malvaceae).

    Zheoat, Ahmed M; Gray, Alexander I; Igoli, John O; Kennedy, Alan R; Ferro, Valerie A

    2017-09-01

    The biologically active title compounds have been isolated from Hibiscus sabdariffa plants, hibiscus acid as a dimethyl sulfoxide monosolvate [systematic name: (2 S ,3 R )-3-hy-droxy-5-oxo-2,3,4,5-tetra-hydro-furan-2,3-di-carb-oxy-lic acid dimethyl sulfoxide monosolvate], C 6 H 6 O 7 ·C 2 H 6 OS, (I), and hibiscus acid dimethyl ester [systematic name: dimethyl (2 S ,3 R )-3-hy-droxy-5-oxo-2,3,4,5-tetra-hydro-furan-2,3-di-carboxyl-ate], C 8 H 10 O 7 , (II). Compound (I) forms a layered structure with alternating layers of lactone and solvent mol-ecules, that include a two-dimensional hydrogen-bonding construct. Compound (II) has two crystallographically independent and conformationally similar mol-ecules per asymmetric unit and forms a one-dimensional hydrogen-bonding construct. The known absolute configuration for both compounds has been confirmed.

  12. Optimization of batch alcoholic fermentation of Cyperus esculentus

    Esuoso, K O [Dept. of Chemistry, Federal Univ. of Technology, Akure (Nigeria); Oderinde, R A [Dept. of Chemistry, Ibadan Univ. (Nigeria); Vega-Catalan, F J [Dept. of Computer Science, Ibadan Univ. (Nigeria); Bamiro, F O [Dept. of Chemical Sciences, Univ. of Agriculture, Abeokuta (Nigeria)

    1993-01-01

    In our earlier work carried out on alcoholic fermentation of molasses the optimum sugar concentration was between 14.0 and 17.0% w/v sugars, beyond which ethanol content decreased drastically. However in the present work, ethanol concentration was still high at 20% w/v sugars. This suggested that the strain had enhanced tolerance to the adverse effects of C. esculentus medium than molasses. Substituting the calculated parameter values (a[sub 1]-a[sub 10]) and optimal conditions of pH, S and T into equation, the calculated yield equals 92.7%. We again tried to verify experimentally the reliability of the theoretical optimal values obtained. The concentration of ethanol obtained was 9.2 [+-] 0.2% v/v. This was higher than the maximum concentration recorded before simulation (8.9% v/v). The experimental yield obtained was 90.3 [+-] 1.6% which is in close agreement with the simulated value of 92.7%. (orig.)

  13. Studies on mutation breeding of hibiscus syriacuse

    Song, Hee Sub; Kim, Jin Kyu; Lee, Ki Un; Lim, Yong Taek [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1995-12-01

    Hibiscus has been known as a national flower of Korea. Hibiscus has ahch a characteristic of self-incompatibility that all the plants exist as natural hybrids and have heterogeneous genes. Thirth two domestic varieties were propagated. Radiosensitivity of H. syriacus irradiated with gamma ray was investigated in plant cuttings. The plant height was reduced by 45 percent in 5 kR irradiated group compared to control group. The radiation dose of 5 kR could be rrecommended for mutation breeding of Hibiscus cuttings. Promising mutant lines were selected form the varieties of Hwarang Wolsan 176, I1pyondansim and Emille. 6 tabs., 2 figs., 13 refs., 4 ills. (Author).

  14. Studies on mutation breeding of hibiscus syriacuse

    Song, Hee Sub; Kim, Jin Kyu; Lee, Ki Un; Lim, Yong Taek

    1995-12-01

    Hibiscus has been known as a national flower of Korea. Hibiscus has ahch a characteristic of self-incompatibility that all the plants exist as natural hybrids and have heterogeneous genes. Thirth two domestic varieties were propagated. Radiosensitivity of H. syriacus irradiated with gamma ray was investigated in plant cuttings. The plant height was reduced by 45 percent in 5 kR irradiated group compared to control group. The radiation dose of 5 kR could be rrecommended for mutation breeding of Hibiscus cuttings. Promising mutant lines were selected form the varieties of Hwarang Wolsan 176, I1pyondansim and Emille. 6 tabs., 2 figs., 13 refs., 4 ills. (Author)

  15. Temperature and modified atmosphere affect the quality of okra Temperatura e atmosfera modificada influenciam a qualidade do quiabo

    Fernando Luiz Finger

    2008-01-01

    Full Text Available Little information is available on the influence of temperature on plastic films wrapped okra (Albelmoschus esculentus for their postharvested conservation. This works investigated the influence of the temperature and PVC film on the development of chilling injury and storability from one of the most popular Brazilian cultivar of okra cv. Amarelinho in fruits stored at 5, 10ºC and at 25ºC. Fruits were harvest at commercial maturity stage with length ranging from 8 to 12 cm, and immediately wrapped in PVC over a polystyrene tray and than stored until visible deterioration or wilting symptoms. Lowering the temperature of storage room from 25 to 10 or 5ºC decreased the weight loss in both PVC wrapped and control fruits, with a lower rate at 5ºC. By reducing the temperature to 5 or 10ºC and wrapping the fruits in PVC film, the relative water content of the fruit pericarp was maintained throughout the storage, while at 25ºC the high weight loss was associated with significant reduction of the water content. The development of chilling symptoms was delayed by the presence of PVC film in fruits stored at 5ºC. However, at 10ºC symptoms of pitting were not developed in PVC wrapped or control fruits up to tenth day of storage. The rate of chlorophyll degradation was diminished by reducing the temperature and by wrapping the fruits with PVC film. The appearance of severe chilling symptoms at 5ºC was associated to less chlorophyll in the fruit pericarp on the control as compared to their content in the PVC wrapped fruits.Para o armazenamento do quiabo (Albelmoschus esculentus há poucas informações disponíveis sobre a influência da temperatura e filmes plásticos na conservação pós-colheita desta hortaliça. Para investigar a influência da temperatura e do filme de PVC na qualidade e desenvolvimento de sintomas de injúria por frio de uma das mais populares cultivares brasileira de quiabo cv. Amarelinho, os frutos foram armazenados a 5, 10

  16. Phytochemical Screening and Antibacterial Activities Of HIBISCUS ...

    USER

    Department of Applied Science, College of Science and Technology, ... results showed the presence of tannins, flavonoids, saponins and steroids in the methanolic extract of the leaves of Hibiscus sabdariffa L. The mean zones of inhibition of ...

  17. the effect of aqueous extract of cyperus esculentus on some liver ...

    honey

    2014-03-31

    Mar 31, 2014 ... Cyperus esculentus is valued for the highly nutritional starch content, dietary fibre and digestible carbohydrate of monosaccharides, disaccharides and polysaccharides ..... Comparative physicochemical evaluation of tigernut, soyabean and coconut milk sources. Int. J. Agric. Biol. 5: 785-787. Burn, M.K. ...

  18. Kinetics of modulatory role of Cyperus esculentus L. on the specific ...

    Background: The continuous search for new lead compounds as viable inhibitors of specific enzymes linked to carbohydrate metabolism has intensified. Cyperus esculentus L. is one of the therapeutically implicated botanicals against several degenerative diseases including diabetes mellitus. Materials and Methods: This ...

  19. 'Lufkin Red' and 'Lufkin White' winter-hardy hibiscus (Hibiscus x laevis All.)

    USDA ARS announces the release of ‘Lufkin Red’ and ‘Lufkin White’ winter-hardy native hibiscuses.Both clones have desirable horticultural traits in combination with demonstrated high levels of field resistance to the leaf spot complex that is problematic on winter-hardy hibiscus clones in areas wit...

  20. Seasonal Canopy Temperatures for Normal and Okra Leaf Cotton under Variable Irrigation in the Field

    James R. Mahan

    2016-11-01

    Full Text Available Temperature affects a number of physiological factors in plants and is related to water use, yield and quality in many crop species. Seasonal canopy temperature, measured with infrared thermometers, is often used in conjunction with environmental factors (e.g., air temperature, humidity, solar radiation to assess crop stress and management actions in cotton. Normal and okra leaf shapes in cotton have been associated with differences in water use and canopy temperature. The okra leaf shape in cotton is generally expected to result in lower water use and lower canopy temperatures, relative to normal leaf, under water deficits. In this study canopy temperatures were monitored in okra and normal leaf varieties for a growing season at four irrigation levels. Differences in canopy temperature (<2 °C were measured between the two leaf shapes. As irrigation levels increased, canopy temperature differences between the leaf shapes declined. At the lowest irrigation level, when differences in sensible energy exchanges due to the okra leaf shape would be enhanced, the canopy temperature of the okra leaf was warmer than the normal leaf. This suggests that varietal differences that are not related to leaf shape may have more than compensated for leaf shape differences in the canopy temperature.

  1. Comparison between the DNA Fingerprints Obtained from the Yellow Vein Mosaic Disease Tolerant Okra Mutants and Their Parental Variety

    Boonsirichai, Kanokporn; Puripunyavanich, Vichai; Phadvibulya, Valailak; Adthalungrong, Amnuai; Srithongchai, Wanphen

    2006-01-01

    The yellow vein mosaic disease (YVMD) is a widespread disease that is found among export orchards of okra. In this report, we studied gamma radiation-induced YVMD tolerant okra mutants and other commercial okra varieties at DNA level. We found that DNA extraction method that utilized sodium dodecyl sulfate and potassium acetate to precipitate other biomolecules was a suitable method to use for DNA finger printing of okra. The MFLP finger printing technique was superior to the AFLP technique in finding polymorphisms among different okra varieties. Also polymorphisms between the YVMD-tolerant mutant lines and their parental variety could be detected, indicating that gamma radiation could induce some changes at DNA level in these plants

  2. Spreading and germination of yellow nutsedge (Cyperus esculentus L.) in Hungary.

    Hoffmann, Z P; Buzsáki, K; Béres, I

    2006-01-01

    Yellow nutsedge (Cyperus esculentus) is a cosmopolitan, tropical, subtropical plant. On the basis of Ujvarosi life-form it is a G2 perennial plant, overwintering with tubers in the soil. It occurs in all continents: along Eastern and Western coastlines of Africa and even in South-Africa, North and South America, Japan, India, Near-Eastern countries, Western, Southern and Eastern Europe. It has been spread since the 70's in Europe, but its remarkable occurrence was between 1980 and 1995 years. Nowadays it occurs in Germany, the Netherlands, Belgium, France, Portugal, Austria, Croatia, Switzerland, Italy and Hungary among the European countries. The first occurrence of C. esculentus was observed in Hungary in 1993, at the surroundings of Keszthely and H&viz towns in a maize-ecosystem (Dancza 1994). It can be presumed, that its import happened with Gladiolus tubers and seed-grain of maize. At present C. esculentus occurs in four regions and surroundings of 20 habitations of Hungary. Somogy county is the most infected area, where it occurs on 10,000 hectares. C. esculentus took the 16th place in the important order of weeds of the world in the 70's. On the basis of EPPO IAS Panel at present this weed specics is considered as one of the most harmful invasive species of the world, due to its severe economic injury. Most harmful effect of C. esculentus is expressed in spring-sown hoed cultures, mainly in maize. Beside this it also occurs in sunflower, potato and sugarbeet cultures. C. esculentus has a good competitive ability by reducing crop quality and quantity. Vegetative reproduction is dominant in spreading but its propagation by seeds is also presumed in Hungary. Cool, rainy weather favours for the vegetative reproduction, while warm, dry one for the flowering. It has a 1-1.5 mm long fruit with one seed. One clustering can contain 600 seeds. According to Lapham (1985) there are areas in Zimbabwe where one can find 100 million C. esculentus seeds in a hectare. At a 1

  3. Factors affecting colonization and abundance of Aphis gossypii glover (hemiptera: aphididae on okra plantations Fatores que afetam a colonização e abundância de Aphis gossypii glover (Hemiptera: Aphididae em plantações de quiabeiro

    Germano Leão Demolin Leite

    2007-04-01

    Full Text Available The control of Aphis gossypii Glover (Hemiptera: Aphididae on okra Abelmoschus esculentus (L. (Malvaceae consist primarily in the use of insecticides, due to the lack of information on other mortality factors. The objective of this study was to determine the effects of predators and parasitoids, height of canopy, plant age, leaf areas, organic compounds leaves, levels of leaf nitrogen and potassium, density of leaf trichomes, total rainfall and median temperature on attack intensity of A. gossypii on two successive A. esculentus var. Santa Cruz plantations. Monthly number estimates of A. gossypii and natural enemies (visual inspection occurred on bottom, middle and apical parts of 30 plants/plantation (one leaf/plant. Plants senescence, leaf areas and natural enemies, mainly Adialytus spp., spiders and Coccinellidae, were some of the factors that most contributed to aphid reduction. A higher number of aphids was found on the bottom part than medium and apical parts of okra plants. Total rainfall can reduce the aphid population. Trichomes non-glandular or low density, organic compounds leaves and levels of N and K were not important for reducing aphid population.O controle de Aphis gossypii Glover (Hemiptera: Aphididae em quiabeiro Abelmoschus esculentus (L. (Malvaceae consiste principalmente no uso de inseticidas, em virtude da falta de informação sobre outros fatores de mortalidade. Objetivou-se com este estudo determinar os efeitos de predadores e parasitóides, altura de dossel, idade da planta, área foliar, compostos orgânicos foliares, níveis de nitrogênio e potássio, densidade de tricomas, pluviosidade e temperatura na intensidade de ataque de A. gossypii em dois cultivos sucessivos de Abelmoschus esculentus var. Santa Cruz. Estimou-se, mensalmente, o número de A. gossypii e de inimigos naturais (inspeção visual ocorridos nas folhas (uma folha/planta localizadas nas partes basal, mediana e apical de 30 plantas/plantação. Os

  4. Influência da colonização micorrízica arbuscular sobre a nutrição do quiabeiro Influence of arbuscular mycorrhiza fungi on the nutrition of okra plant

    Ricardo Luís Louro Berbara

    1999-09-01

    Full Text Available Foram estudados em casa de vegetação alguns parâmetros de crescimento em plantas de quiabo (Abelmoschus esculentus (L. Moench cv. Piranema colonizadas por dois grupos de fungos micorrízicos arbusculares, com o objetivo de determinar a influência dos inóculos na nutrição e morfologia radicular do quiabeiro. Um grupo continha apenas esporos de Acaulospora longula (A enquanto o outro, esporos de oito espécies: Glomus occultum, Glomus aggregatum, Glomus microcarpum, Acaulospora longula, Acaulospora morrowae, Sclerocystis coremioides, Sclerocystis sinuosa, Scutellospora pellucida. As plantas foram submetidas a três níveis de P (0, 10 e 60 kg ha-1 de P2O5 e coletadas em três diferentes idades (22, 32 e 47 dias, com quatro repetições para cada tratamento. Foi determinado o acúmulo de N, P, K, e Mg na raiz e parte aérea, bem como o influxo médio desses elementos e a área radicular. Os resultados indicaram, além da resposta positiva do quiabeiro ao P, uma maior eficiência da inoculação com mistura de espécies apesar de o influxo médio, determinado aos 47 dias, apresentar maiores valores para o tratamento com A. longula.An experiment was carried out in greenhouse to determine the influence of inoculation of two groups of arbuscular mycorrhizae on the nutrition and radicular morphology of the okra plant (Abelmoschus esculentus (L. Moench cv. Piranema. One group had only Acaulospora longula spores and the other a spore mixture of eight species: Glomus occultum, Glomus aggregatum, Glomus microcarpum, Acaulospora longula, Acaulospora morrowae, Sclerocystis coremioides, Sclerocystis sinuosa, Scutellospora pellucida. The experiment was held in greenhouse conditions with three levels of P (0, 10 and 60 kg ha-1 of P2O5, three samplings dates (22, 32 and 47 days and four replications. The accumulation of N, P, K, Ca and Mg in roots and shoots, root area and their influx ratio were determined. The results made evident that the mixture of

  5. Effect of Sources of Organic Manure on Growth and Yields of Okra ...

    and Poultry Manure) on growth and yield of okra was carried out at the Teaching and Research Fadama Farm of Usmanu ... in Asia (Agboola and Omueti, 1985). According to ... use renewable forms of energy and reduce costs of fertilizing ... MATERIALS AND METHODS ... area and were composited, air-dried and sieved.

  6. Effect of intercropping varieties of sweet potato and okra in an ultisol ...

    SERVER

    2007-07-18

    Jul 18, 2007 ... 2Department of Agronomy, College of Crop and Soil Sciences, Michael ... Land equivalent ratio was higher with intercropping TAE 38 okra and TIS ... to the use of low yield crop cultivars, resulting in low yield ... The top soil (0 - 20 cm) texture of .... sweet potato conserved soil moisture and reduced weed.

  7. Suitability of Protein-Rich Extract from Okra Seed for Formulation of ...

    After 21 days, the rats were decapitated and blood samples harvested. The blood was used for the haematological tests. Liver and heart toxicity indicator including aspartate amino transferase (AST) and alanine amino transferase (ALT) were assayed. Ready-to-use therapeutic food (RUTF) prepared with PRE of okra seeds ...

  8. Effect of cropping system on yield of some sweetpotato and okra ...

    A field trial was conducted for 2 years to determine cropping system effect on yield of some sweetpotato and okra cultivars in an intercropping system. Sweetpotato variety TIS 87/0087 produced significantly (P<0.05) higher number of tubers per plant than Tis 8164 and Tis 2532.op.1.13 varieties. Tuber yield was also ...

  9. Effects of rice batter on oil uptake and sensory quality coated fried okra.

    Okra was coated and deep-fat fried with batters of flour sources, including rice flour, a mixture of rice flour, and small amounts of pregelatinized rice flour (PGRF), and, as a control, the traditional wheat flour. The addition of PGRF, up to 8%, enhanced batter viscosity and the coating properties...

  10. Effect of Sources of Organic Manure on Growth and Yields of Okra ...

    Cow, Sheep and Poultry Manure) on growth and yield of okra was carried out at the Teaching and Research Fadama Farm of Usmanu Danfodiyo University Sokoto, Nigeria during 2007/2008 and 2008/2009 dry seasons. Treatments were laid ...

  11. Cytological maps of lampbrush chromosomes of European water frogs (Pelophylax esculentus complex) from the Eastern Ukraine

    2013-01-01

    Background Hybridogenesis (hemiclonal inheritance) is a kind of clonal reproduction in which hybrids between parental species are reproduced by crossing with one of the parental species. European water frogs (Pelophylax esculentus complex) represent an appropriate model for studying interspecies hybridization, processes of hemiclonal inheritance and polyploidization. P. esculentus complex consists of two parental species, P. ridibundus (the lake frog) and P. lessonae (the pool frog), and their hybridogenetic hybrid – P. esculentus (the edible frog). Parental and hybrid frogs can reproduce syntopically and form hemiclonal population systems. For studying mechanisms underlying the maintenance of water frog population systems it is required to characterize the karyotypes transmitted in gametes of parental and different hybrid animals of both sexes. Results In order to obtain an instrument for characterization of oocyte karyotypes in hybrid female frogs, we constructed cytological maps of lampbrush chromosomes from oocytes of both parental species originating in Eastern Ukraine. We further identified certain molecular components of chromosomal marker structures and mapped coilin-rich spheres and granules, chromosome associated nucleoli and special loops accumulating splicing factors. We recorded the dissimilarities between P. ridibundus and P. lessonae lampbrush chromosomes in the length of orthologous chromosomes, number and location of marker structures and interstitial (TTAGGG)n-repeat sites as well as activity of nucleolus organizer. Satellite repeat RrS1 was mapped in centromere regions of lampbrush chromosomes of the both species. Additionally, we discovered transcripts of RrS1 repeat in oocytes of P. ridibundus and P. lessonae. Moreover, G-rich transcripts of telomere repeat were revealed in association with terminal regions of P. ridibundus and P. lessonae lampbrush chromosomes. Conclusions The constructed cytological maps of lampbrush chromosomes of P

  12. Pecular Features of Hematopoiesis in the Liver of Mature and Immature Green Frogs (Pelophylax Esculentus Complex

    Akulenko N. M.

    2016-12-01

    Full Text Available The article describes characteristic features of the hematopoiesis in mature and immature green frogs (Pelophylax esculentus complex. Quantitative differences in liver myelograms were insignificant. However, in a sample of mature animals numerous significant correlations between the number of pigment inclusions in the liver and indicators of erythropoiesis and myelopoiesis were observed. Those correlations were absent in the immature frogs. We concluded that aft er the frogs’ breeding a lack of plastic resources, in particular, hemosiderin remains up to the hibernation.

  13. Variability of Structural and Biomechanical Parameters of Pelophylax Esculentus (Amphibia, Anura Limb Bones

    Broshko Ye. O.

    2014-07-01

    Full Text Available Variability of Structural and Biomechanical Prameters of Pelophylax esculentus (Amphibia, Anura Limb Bones. Broshko Ye. O. — Structural and biomechanical parameters of Edible Frog, Pelophylax esculentus (Linnaeus, 1758, limb bones, namely, mass, linear dimensions, parameters of the shaft’s cross-sectional shape (cross-sectional area, moments of inertia, radiuses of inertia were investigated. Some coefficients were also estimated: diameters ratio (df/ds, cross-sectional index (ik, principal moments of inertia ratio (Imax/Imin.. Coefficients of variation of linear dimensions (11.9-20.0 % anrelative bone mass (22-35 % were established. Moments of inertia of various bones are more variable (CV = 41.67-56.35 % in relation to radii of inertia (CV = 9.68-14.67 %. Shaft’s cross-sectional shape is invariable in all cases. However, there is high individual variability of structural and biomechanical parameters of P. esculentus limb bones. Variability of parameters was limited by the certain range. We suggest the presence of stable norm in bone structure. Stylopodium bones have the primary biomechanical function among the elements of limb skeleton, because their parameters most clearly responsive to changes in body mass.

  14. DESENVOLVIMENTO E PRODUÇÃO DO QUIABEIRO EM FUNÇÃO DAS DATAS DE PLANTIO GROWTH AND YIELD OF OKRA AS INFLUENCED BY PLANTING DATES

    Peter Ernst Sonnenberg

    2007-09-01

    Full Text Available

    A influência de quatro épocas de plantio (15 de maio, 15 de junho, 15 de julho e 15 de agosto de 1998 no desenvolvimento e na produção do quiabeiro (Abelmoschus esculentus (L Moench (cv. Santa Cruz 47 foi investigada em Goiânia, GO. O experimento foi conduzido na Escola de Agronomia e Engenharia de Alimentos da Universidade Federal de Goiás, em Latossolo Vermelho Amarelo, cultivado há muitos anos. O delineamento experimental foi o de blocos casualizados com quatro repetições. Foram encontradas diferenças significativas (Tukey 5% entre épocas de plantio, para as seguintes características: número de frutos produzidos durante as trinta primeiras colheitas, altura das plantas no início da colheita, número de ramos laterais e número de dias da semeadura ao início do florescimento, ao início da colheita e ao início da colheita em 75% das covas. A temperatura média dos meses seguintes às datas de plantio aumentou de maio para agosto. Observou-se uma redução do período da semeadura até o início do florescimento, até o início da colheita e até o início da colheita em 75% das covas. O número de ramos laterais também foi reduzido no mesmo período. Entretanto verificou-se aumento na altura das plantas e no rendimento das primeiras trinta colheitas.

    PALAVRAS-CHAVE: Abelmoschus esculentus; data de plantio; épocas de plantio.

    The influence of four sowing dates (May 15th, June 15th, July 15th and August 15th, 1998 on the growth and yield of okra (Abelmoschus

  15. Development of post-harvest protocol of okra for export marketing.

    Dhall, R K; Sharma, S R; Mahajan, B V C

    2014-08-01

    The study was carried out on the harvesting and handling methods of okra with the objective to maintain the best quality of pods from harvesting to end consumer especially for export marketing. For that purpose okra cv. 'Punjab-8' pods were harvested with minimum handling (least injuries to the pubescence on the ridges of pod) and normal handling (no safety taken to prevent injuries on pods). Pods were precooled at 15 ± 1ºC, 90-95% RH; jumble packed in the CFB boxes of 2.0 Kg capacity and than stored at 8 ± 1ºC, 90-95% RH. The quality parameters of okra namely texture, chlorophyll content, physiological loss in weight, rotting percentage and general appearance were studied. The pods harvested with minimum handling and field packaging can retain their green colour, crisp texture (maximum force to puncture pod = 500.2 g) with minimum rotting (3.0%) and physiological loss in weight (15.8%) and good appearance upto 13 days of cold storage whereas normal handled pods can be stored upto 5 days at 8 ± 1ºC, 90-95% RH and thereafter lost their general appearance on the 7th day of storage and were discarded. Therefore, in order to maintain high quality of okra from harvesting to the final destination (consumer), the okra pods should be harvested with minimum handling followed by field packaging in CFB boxes.

  16. Pre-germinative treatments in okra seeds in different stadiums of fruit maturationTratamentos pré-germinativos em sementes de quiabo em diferentes estádios de maturação do fruto

    Célia Maria Peixoto de Macedo

    2011-12-01

    Full Text Available The objective of this work was to evaluate the influence of the pre-soak and of the thermotherapy in the germination and in the energy of the okra seeds (Abelmoschus esculentus in different stadiums of maturation of the fruit. The design used was the entirely randomized with four repetitions, in a factorial outline 5 x 9, five maturation stadiums (green, semi-hard, hard, dry and dehiscent and nine treatments pre-germination: pre-soak for immersion of the seeds in water to the temperature of 30ºC for 6, 12 and 24 hours; immersion of the seeds in water to 40, 50, 60 and 70ºC for 3 minutes; thermotherapy for immersion of the seeds in water to the ebullition temperature (97ºC until cooling and intact seeds (control. The seeds were sowed in paper-towel rolls and they were maintained in camera type BOD regulated to 20-30ºC and photoperiod of 8-16 hours. The okra seeds originating from dry and dehiscent fruits presented larger germination performance. The pre-germination treatments more effective to accelerate the germination of seeds obtained from dry and dehiscent fruits are the pre-soak for 6 hours and thermotherapy at 60 °C for 3 minutes, respectively.O objetivo deste trabalho foi avaliar a influência da pré-embebição e da termoterapia na germinação e no vigor das sementes de quiabo (Abelmoschus esculentus em diferentes estádios de maturação do fruto. O delineamento foi o inteiramente casualizado com quatro repetições, num esquema fatorial 5 x 9, cinco estádios de maturação (verde, semi-duro, duro, seco e deiscente e nove tratamentos pré-germinativos: pré-embebição por imersão das sementes em água à temperatura de 30ºC por 6, 12 e 24 horas; imersão das sementes em água a 40, 50, 60 e 70ºC por 3 minutos; termoterapia por imersão das sementes em água à temperatura de ebulição (97ºC até esfriar e sementes intactas (testemunha. As sementes foram semeadas em rolos de papel-toalha que foram mantidos em câmara tipo

  17. Preliminary characterization of residual biomass from Hibiscus ...

    Hibiscus sabdariffa calyces are mainly used for different agro-food and beverages applications. The residual biomass generated contains various useful substances that were extracted and characterized. It contained 23% (w/w) soluble pectic material, a food additive, extracted with hot acidified water (80°C, pH = 1.5) and ...

  18. Phytochemical Screening and Antibacterial Activities of Hibiscus ...

    The phytochemical properties and the antibacterial potency of rosselle (Hibiscus sabdariffa L) leaf extracts were evaluated using the cold maceration method, agar diffusion method and qualitative phytochemical analysis respectively. The methanolic extract was tested against Salmonella typhi, Escherichia coli and ...

  19. Detection of somaclonal variation in micropropagated Hibiscus ...

    The main objective of micropropagation is to produce clones i.e. plants which are phenotypically and genetically identical to the mother plants. The culture of organized meristems usually guarantees the production of true-to-type plants but variations in the progenies have been widely reported. Hibiscus sabdariffa L. plants ...

  20. The Hibiscus panduriformis complex (Malvaceae) in Australia

    Juswara, L.S.; Craven, L.A.

    2005-01-01

    The Hibiscus panduriformis Burm.f. species complex in Australia is revised. Six species are recognised, of which one is the widespread H. panduriformis; one, H. austrinus, is based upon H. panduriformis var. australis; and four represent new species, H. apodus, H. calcicola, H. fluvialis, and H.

  1. Can hibiscus tea lower blood pressure

    Hibiscus sabdariffa is a common ingredient found in blended herbal teas, and beverages made from the dried calyces of this plant are popular worldwide. In vitro studies have shown that H. sabdariffa has antioxidant properties and, in animal models of hypertension, extracts of this plant lower blood ...

  2. Inheritance of characters in kenaf (Hibiscus cannabinus)

    SERVER

    2008-04-03

    David and Adam, 1988). The plant belongs to the order Malvales, the family Malvaceae and the genus Hibiscus ... The varieties in relation to the character studied are as follows: NRG-NG-R5: Pink flowers with resistance to root ...

  3. Characterisation of twenty-nine (29) accessions of okra (Abelmoschus spp (L.) Moench) in Ghana

    Ahiakpa, J. K.

    2012-01-01

    A series of investigations were carried out to determine the genetic variability within 29 accessions of okra (Abelmoschus spp (L.) Moench) through characterisation using morphological, biochemical, nutritional and molecular markers. The goal was to obtain information on key traits of okra germplasm relevant to breeders and other researchers towards improvement of the crop. Twenty six (26) indigenous (landraces) and three (3) exotic accessions of okra were collected from eight regions of Ghana and their morpho-agronomic traits were evaluated under field conditions at the Biotechnology and Nuclear Agriculture Research Institute (BNARI) research fields using the International Plant Genetic Research Institute (IPGRI) descriptor list for okra. The 29 exhibited significant variation in all but two quantitative traits studied. Block coefficients of variation were extremely low, implying that results obtained are reliable and repeatable over replications. The 29 accessions were grouped into two major clusters and subsequently into five sub-clusters based on both quantitative and qualitative characters studied. The association between pairs of quantitative yield traits in the okra landraces revealed that flowering and fruiting parameters had significant (P < 0.01) positive associations. Factor scores of 12 characters contributed substantially to total genetic variation among the 29 okra accessions studied. The pattern of clustering did not show distinct association between morpho-agronomic characters and geographic origin of the collections. The output of the Principal Components Analysis (PCA) revealed that different characters contributed differently to total genetic variation. The means of maximum viscosity values for mucilage extracted from the fruits ranged from 53.0 - 366.8bu, with three accessions; DKA (366.8bu), Yeji-Local (329bu) and Amanfrom (316.8bu) recording very high values whilst Cape (53.0bu) had the least maximum viscosity value. There was low level of

  4. Response of okra to sodium nutrition in an Ultisol | Aduayi | Moor ...

    The essentiality of Na to the growth of okra plants in an Ultisol grown in soil culture and treated with Na concentrations of 0, 2, 4, 8, 16 mg/kg of soils as NaCl. was studied. Sodium applied at 8mg/kg of soil increased leaf area and number, plant height, water content dry matter yield and tissue N and K when compared with ...

  5. Seedling imaging analysis and traditional tests to assess okra seed vigor

    Kikuti,Ana Lúcia Pereira; Marcos-Filho,Júlio

    2013-01-01

    Seed vigor testing is an important component of quality control programs adopted by seed industry. The software Seed Vigor Imaging System (SVIS) has been successfully used for seed vigor assessment in different species. The objective of this research was to verify the SVIS efficiency to assess okra seed vigor in comparison to other vigor tests used for this species. Five seed lots of 'Clemson Americano' and four of 'Santa Cruz' were submitted to germination (speed and percentage), cold germin...

  6. Nonwoven production from agricultural okra wastes and investigation of their thermal conductivities

    Duman, M. N.; Kocak, E. D.; Merdan, N.; Mistik, I.

    2017-10-01

    Nowadays bio-based composite materials have been used in rising amounts and demanded widely in industrial uses, as they provide cost reduction and weight loss in the end use products. Agricultural cellulose based wastes can be a good alternative to synthetic fibers and can be used in natural fiber reinforced composite production, as there is a huge (more than 40 million tons) potential for natural cellulose production from agricultural wastes. Okra is one of the most grown vegetables around the world with stems left on the fields after harvest. When the similarity of mechanical properties of okra fibers with traditional bast fibers (flax, kenaf, hemp) are considered, from an economical and an environmental point of view this research emphasizes the potential of agricultural biomass for natural fiber production. In this study, okra stem wastes used for natural cellulosic fiber production and treated with 10% NaOH at 60°C for 10, 20, 30 and 40 minutes. By alkali treatment, decrease in fiber diameter and weight, and increase in tensile strength and elongation % have been observed. Nonwoven production has been done from both the fibers with and without surface treatments. Thermal conductivity properties of both nonwovens have been investigated.

  7. Persistence and risk assessment of emamectin benzoate residues on okra fruits and soil.

    Jyot, Gagan; Mandal, Kousik; Chahil, G S; Singh, Balwinder

    2014-08-01

    Emamectin benzoate, a synthetic derivative of abamectin, is found effective against fruit borer and jassid in okra crops. The present studies were carried out to study the dissipation pattern of emamectin benzoate on okra and to suggest a suitable waiting period for the safety of consumers. Following three applications of emamectin benzoate (Proclaim 5 SG) at 68.1 and 136.2 g a.i. ha-1, the average initial deposits of emamectin benzoate were observed to be 0.22 and 0.42mg kg-1, respectively. These residues dissipated below the limit of quantification (LOQ) of 0.05 mg kg-1 after 5 days at both the dosages. Soil samples collected after 15 days did not reveal the presence of emamectin benzoate at LOQ of 0.05 mg kg-1. Acceptable daily intake (ADI) of emamectin benzoate is 0.0005 mg kg-1 body weight day-1, which means an adult of 55 kg weight can safely tolerate an intake of 27.50 microg emamectin benzoate. Assuming an average consumption of 80 g okra fruit and multiplying it by average and maximum residues observed on 0 day at recommended dosage, the intake of emamectin benzoate comes out to be about 20 Itg and these values are quite safe in comparison to its ADI. These studies, therefore, suggest that the use of emamectin benzoate at the minimum effective dosages do not seem to pose any hazards to the consumers if a waiting period of 1 day is observed.

  8. Radiosensitivity and chimera formation in Hibiscus syriacus

    Kwon, S.H.; Won, J.L.

    1980-01-01

    Radiosensitivity of gamma-irradiated Hibiscus syriacus and chimera formation were investigated. The lethal dose-50 percent with respect to seeding and cuttings was 15kR and 2 approximately 3 kR respectively, chlorophyll mutation rate of seeds irradiated with 15 kR being about 13 percent. The degree of chimeric leaf mutants from the buds by radiation treatment depends on the bud position of the branch. Buds of the middle part of V 1 branch seemed to be more multi-cellular condition than the upper and low part when irradiation was made. It is assumed that at least two primordia of V 2 branch were already differentiated from the V 1 branch in Hibiscus syriacus plant. (Author)

  9. Effects of aqueous extracts of Hibiscus sabdariffa calyces and ...

    The effects of aqueous extracts of the leaves of Ocimum gratissimum and calyces of Hibiscus sabdariffa on intestinal transit were determined in experimental rats The leaves of Ocimum gratissimum were oven dried and then pulverized. The dried calyces of Hibiscus sabdariffa were also pulverized. 10% extracts of both ...

  10. PHARMACOLOGICAL EFFECTS AND THERAPEUTIC PROPERTIES OF HIBISCUS CANNABINUS- A REVIEW

    Ali Esmail Al-Snafi

    2018-01-01

    The phytochemical analysis of Hibiscus cannabinus showed the presence of phytosterols, flavonoids, polyphenols, tannins, steroids, alkaloids, saponins, lignans, essential oils, glucosides such as cannabiscitrin, cannabiscetin and anthocyanin glycoside. The pharmacological studies revealed that Hibiscus cannabinus possessed cytotoxic, anthelmintic, antibacterial, antiulcer, antidiabetic, hypolipidemic, antioxidant, immunological, haematinic and hepatoprotective effects. This review will highli...

  11. A cilevirus infects ornamental hibiscus in Hawaii.

    Melzer, Michael J; Simbajon, Nelson; Carillo, James; Borth, Wayne B; Freitas-Astúa, Juliana; Kitajima, Elliot W; Neupane, Kabi R; Hu, John S

    2013-11-01

    The complete nucleotide sequence of a virus infecting ornamental hibiscus (Hibiscus sp.) in Hawaii with symptoms of green ringspots on senescing leaves was determined from double-stranded RNA isolated from symptomatic tissue. Excluding polyadenylated regions at the 3' termini, the bipartite RNA genome was 8748 and 5019 nt in length for RNA1 and RNA2, respectively. The genome organization was typical of a cilevirus: RNA1 encoded a large replication-associated protein with methyltransferase, protease, helicase and RNA-dependent RNA polymerase domains as well as a 29-kDa protein of unknown function. RNA2 possessed five open reading frames that potentially encoded proteins with molecular masses of 15, 7, 62, 32, and 24 kDa. The 32-kDa protein is homologous to 3A movement proteins of RNA viruses; the other proteins are of unknown function. A proteome comparison revealed that this virus was 92 % identical to citrus leprosis virus cytoplasmic type 2 (CiLV-C2), a recently characterized cilevirus infecting citrus with leprosis-like symptoms in Colombia. The high sequence similarity suggests that the virus described in this study could be a strain of CiLV-C2, but since the new genus Cilevirus does not have species demarcation criteria established at present, the classification of this virus infecting hibiscus is open to interpretation. This study represents the first documented case of a cilevirus established in the United States and provides insight into the diversity within the genus Cilevirus.

  12. A cilevirus infects ornamental hibiscus in Hawaii

    Melzer, Michael J.; Simbajon, Nelson; Carillo, James; Borth, Wayne B.; Freitas-Astúa, Juliana; Kitajima, Elliot W.; Neupane, Kabi R.; Hu, John S.

    2013-01-01

    The complete nucleotide sequence of a virus infecting ornamental hibiscus (Hibiscus sp.) in Hawaii with symptoms of green ringspots on senescing leaves was determined from double-stranded RNA isolated from symptomatic tissue. Excluding polyadenylated regions at the 3′ termini, the bipartite RNA genome was 8748 and 5019 nt in length for RNA1 and RNA2, respectively. The genome organization was typical of a cilevirus: RNA1 encoded a large replication-associated protein with methyltransferase, protease, helicase and RNA-dependent RNA polymerase domains as well as a 29-kDa protein of unknown function. RNA2 possessed five open reading frames that potentially encoded proteins with molecular masses of 15, 7, 62, 32, and 24 kDa. The 32-kDa protein is homologous to 3A movement proteins of RNA viruses; the other proteins are of unknown function. A proteome comparison revealed that this virus was 92% identical to citrus leprosis virus cytoplasmic type 2 (CiLV-C2), a recently characterized cilevirus infecting citrus with leprosis-like symptoms in Colombia. The high sequence similarity suggests that the virus described in this study could be a strain of CiLV-C2, but since the new genus Cilevirus does not have species demarcation criteria established at present, the classification of this virus infecting hibiscus is open to interpretation. This study represents the first documented case of a cilevirus established in the United States and provides insight into the diversity within the genus Cilevirus. PMID:23732930

  13. Mutagenesis of hibiscus rosa-sinensis

    Shuhaimi Shamsudin; Shakinah Salleh; Mohd Nazir Basiran

    2005-01-01

    Mutation induction is an alternative method to create more variation in Hibiscus rosa-sinensis. Radiosensitivity test was carried out to determine the effective doses for irradiation of stem cuttings. Stem cuttings of Hibiscus rosa-sinensis were irradiated at 10, 20, 30 and 60 Gy using a gamma cell with a Co-60 source at a dose rate of 1.66 Gys -1 . Irradiated stem cuttings were planted in sand-beds and data on the number of growing shoots were taken. Increasing gamma ray doses resulted in a reduction of growing shoots on the irradiated stem cuttings. It was shown that the LD50 for the stem cuttings was 36.2 Gy and at 75% growth was 17.15 Gy. Based on these results, 20 to 30 Gy were chosen for irradiation of the stem cuttings to induce mutation in Hibiscus rosa-sinensis. Irradiated stem cuttings were then planted into sand-beds for rooting. After one month, the rooted stems were transferred into polybags and allowed to grow under 70% shade provided by plastic netting and the variants were subsequently observed. Three variants with variation in flower shapes and colours were obtained. (Author)

  14. Effect of gamma rays on fruit weight and number of seeds in Abelmoschus esculentus (L.) Moench and Momordica charantia L

    Jha, B.K.

    1994-01-01

    Among 5,15,30,60,90 and 120 kR doses of gamma rays, lower doses showed stimulatory effects on fresh and dry weight of fruit, while higher doses proved inhibitory in Abelmoschus esculentus and Momordica charantia. Abortion of mature seeds was also higher at 30 kR and above doses. (author). 12 refs., 2 tabs

  15. Identification of parental chromosomes in hybridogenetic water frog Pelophylax esculentus (Rana esculenta) by genomic in situ hybridization (GISH)

    Zalésna, A.; Choleva, Lukáš; Ogielska, M.; Rábová, Marie; Marec, František; Ráb, Petr

    2010-01-01

    Roč. 18, č. 16 (2010), s. 754-755 ISSN 0967-3849. [19th International Colloquium on animal cytogenetics and gene mapping. 06.06.-09.06.2010, Krakow] Institutional research plan: CEZ:AV0Z50450515; CEZ:AV0Z50070508 Keywords : parental chromosomes * Pelophylax esculentus * hybridization Subject RIV: EB - Genetics ; Molecular Biology

  16. 'USS Alabama', 'USS Mississippi', 'USS Missouri', 'USS Tennessee' and 'USS Texas' Chinese hibiscus (Hibiscus rosa-sinenis L.)

    Five new Chinese hibiscus cultivars are products from a planned hibiscus breeding program conducted by the inventors at Poplarville, MS. The goal of the research is to produce new clones with a combination of desirable horticultural traits. Desirable floral traits include early bloom, uniform flower...

  17. Competição inicial entre Cyperus esculentus e arroz irrigado em condições de casa-de-vegetação Competition between Cyperus esculentus and irrigated rice under green house conditions

    E. A. L. Erasmo

    2000-08-01

    Full Text Available Com o objetivo de avaliar o efeito competitivo de Cyperus esculentus sobre o crescimento inicial da cultura do arroz irrigado, foi instalado um experimento em casa-devegetação, na Estação Experimental da Faculdade de Agronomia - UNITINS, no munícipio de Gurupi-TO. O delineamento estatístico utilizado foi um fatorial 5x4 com três repetições completamente casualizado. Os tratamentos constaram de cinco períodos de convivência do arroz com C. esculentus (15, 25, 35, 45 e 60 dias após a emergência da cultura e quatro densidades de C. esculentus (0, 2, 4 e 8 tubérculos/vaso, correspondentes a 0; 71; 142 e 286 plantas de C. esculentus por m2, respectivamente. No final de cada período foram avaliados na cultura do arroz os seguintes parâmetros: matéria seca de plantas/vaso; área foliar/planta; matéria seca de perfilhos/vaso; n.º de perfilhos/vaso e altura média de plantas. Os resultados mostraram que o efeito da presença de C. esculentus foi mais marcante nas densidades de quatro e oito tubérculos/vaso a partir dos 35 dias de convivência. O parâmetro mais afetado foi a matéria seca de plantas/vaso, como resultado do decréscimo do número de perfilhos/vaso. A altura das plantas de arroz irrigado não foi afetada pela presença da planta daninha em nenhum período de convivência.With the objective of evaluating the competitive effect of Cyperus esculentus on the growth and production of the culture of irrigated rice, experiments were installed in green house, in the Experimental Station of the School of Agronomy - UNITINS, in Gurupi, State of Tocantins, Brazil. The statistical design used in the experiments was a factorial plot 5x4 with three replications, completely randomized. The treatments consisted of five periods of coexistence of the rice with Cyperus esculentus (15, 25, 35, 45 and 60 days after the emergency - D.A.E, of the rice and four densities of C. esculentus (0, 2, 4 and 8 tuber/pots corresponding to 0; 71; 142 e

  18. Effect of cooking methods on the micronutrient profile of selected ...

    Effect of cooking methods on the micronutrient profile of selected vegetables: okra fruit ( Abelmoshcus esculentus ), fluted pumpkin ( Telfairia occidentalis ), African spinach ( Amarantus viridis ), and scent leaf ( Ocumum gratissimum.

  19. Antioxidant and drug detoxification potentials of Hibiscus sabdariffa anthocyanin extract.

    Ajiboye, Taofeek O; Salawu, Nasir A; Yakubu, Musa T; Oladiji, Adenike T; Akanji, Musbau A; Okogun, Joseph I

    2011-04-01

    The antioxidant and drug metabolizing potentials of Hibiscus anthocyanin extract in CCl(4)- induced oxidative damage of rat liver was investigated. Hibiscus anthocyanin extract effectively scavenge α-diphenyl-β-picrylhydrazyl (DPPH) radical, superoxide ion, and hydrogen peroxide. It produced a 92% scavenging effect of DPPH radical at a concentration of 2.0 mg/mL. Hibiscus anthocyanin extract produced a 69 and 90% scavenging effect on superoxide ion and hydrogen peroxide, respectively, at 1.0 mg/mL, which compared favorably with the synthetic antioxidant (butylated hydroanisole and α-tocopherol). A reducing power of this anthocyanin was examined using K(3)Fe(CN)(6). Hibiscus anthocyanin extract has reducing power that is approximately 2-fold that of the synthetic antioxidant, butylated hydroanisole. Hibiscus anthocyanin extract produced a significantly increase and completely attenuated the CCl(4)-mediated decrease in antioxidant enzymes (e.g., catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase). However, the level of nonenzymic antioxidant molecules (i.e., vitamins C and E) were significant preserved by Hibiscus anthocyanin extract. There was an induction of phase II drug-detoxifying enzymes: glutathione S-transferase, NAD(H):quinone oxidoreductase, and uridyl diphosphoglucuronosyl transferase by 65, 45, and 57%, respectively. In view of these properties, Hibiscus sabdariffa anthocyanin extract can act as a prophylactic by intervening as a free radical scavenger both in vitro and in vivo as well as inducing the phase II drug detoxification enzymes.

  20. Organophosphate Pesticide Residues in Okra and Brinjal Grown in Peri-Urban Environment of Big Cities of Punjab

    Randhawa, M.A.; Zaman, M.; Anjum, F.M.; Asghar, A.

    2015-01-01

    Peri urban farming system is main supplier of vegetables to urban population and organophosphate pesticides exposure due to consumption of okra and brinjal to urban population has been estimated in this study. Okra and brinjal samples (90 samples for each vegetable) were collected from peri-urban farming area of Faisalabad, Multan and Gujranwala and were analysed by GC-ECD for quantification of chlorpyrifos, profenophos and triazophos residues. Data were statistically analyzed to calculate, mean and standard deviation. Quantification of pesticides residues revealed that 85 (47.22 percentage) samples were contaminated and 15 (8.33 percentage of total samples and 17.64 percentage of contaminated) samples showed residues value higher than MRL'S. Out of 85 contaminated samples 27 (31.76 percentage) samples were contaminated with chlorpyrifos, 30 (35.29 percentage) samples with profenofos and 29 (34.11) were contaminated with triazophos. The mean concentration of chlorpyrifos in okra was 0.192 mg Kg-1 and brinjal 0.197 mg Kg-1. The mean concentration for profenofos in okra was 0.02 mg Kg-1 and brinjal 0.035 mg Kg-1. Similarly mean concentration for triazophos in okra was 0.009 mg Kg-1 and brinjal 0.01 mg Kg-1. Overall, 6.67 percentage samples of okra and 6.66 percentage samples of brinjal contaminated with chlorpyrifos exceeded EU MRL of 0.05 mg kg-1. Okra samples were found contaminated at frequency of 4.44 percentage and brinjal samples at frequency of 5.56 percentage with higher profenofos residues level than MRL of 0.05 mg kg-1. Approximately, 6.67 percentage samples of okra and 5.6 percentage samples of brinjal had profenofos residue level above MRL of 0.01 mg kg-1. This study may be helpful for building database regarding regional exposure of urban population and facilitate in estimation of possible risk to their health in our daily life. (author)

  1. TIGER NUT (CYPERUS ESCULENTUS: SOURCE OF NATURAL ANTICANCER DRUG? BRIEF REVIEW OF EXISTING LITERATURE.

    Elom Seyram Achoribo

    2017-07-01

    Full Text Available In some parts of the world, Cyperus esculentus L. is widely used as a healthy food for both humans and animals due to their nutritional and functional properties. Current research and reviews on this plant have focused mainly on organoleptic properties, phytochemical compositions, oil content, biochemical activities, and nutritional values. The medicinal properties of Tiger nut are seldom discussed, although its medicinal use is well known in folklore activities. To explore the medicinal properties of Tiger nut, This review tries to investigate the potential anticancer properties of components issued from Tiger nut by reviewing the existing literature in the field. Based on the evidence from the review, it is recommended that there is a need for further investigation into the proposed anticancer properties of Tiger nut.

  2. Identifying salt stress-responsive transcripts from Roselle ( Hibiscus ...

    Hibiscus sabdariffa L.). Identifying the potentially novel transcripts responsible for salt stress tolerance in roselle will increase knowledge of the molecular mechanism underlying salt stress responses. In this study, differential display reverse ...

  3. Aqueous extract of Hibiscus sabdarrifa calyx alleviates anemia and ...

    Aqueous extract of Hibiscus sabdarrifa calyx alleviates anemia and organ damage in Trypanosoma brucei brucei infected rats. IA Umar, E Daikwo, NG Maryoms, A Gidado, LB Buratai, FS Saka, MA Ibrahim ...

  4. Bioremediation of textile effluent polluted soil using kenaf (Hibiscus ...

    DR BADA

    Bioremediation of textile effluent polluted soil using kenaf (Hibiscus cannabinus Linn.) and composted ... Lead, Cadmium, Chromium and Zinc levels in plants and soil were determined using Atomic ..... Contaminated land in the EC: Report of ...

  5. The Aqueous Calyx Extract of Hibiscus sabdariffa Lowers Blood ...

    olayemitoyin

    Pressure and Heart Rate via Sympathetic Nervous System. Dependent ... discharge of the sympathetic nervous system. Keywords: Hibiscus ... The constituents of HS include protein, fat, ... Talata Mafara central market, Zamfara state, Nigeria.

  6. Bioremediation of textile effluent polluted soil using kenaf ( Hibiscus ...

    Bioremediation of textile effluent polluted soil using kenaf ( Hibiscus cannabinus Linn.) and composted ... Journal of Applied Sciences and Environmental Management. Journal Home ... Twelve-litre plastic pots were filled with 10 kg soil.

  7. Study of large nonlinear change phase in Hibiscus Sabdariffa

    Trejo-Durán, M.; Alvarado-Méndez, E.; Andrade-Lucio, J. A.; Rojas-Laguna, R.; Vázquez-Guevara, M. A.

    2015-09-01

    High intensities electromagnetic energy interacting with organic media gives rise to nonlinear optical effects. Hibiscus Sabdariffa is a flower whose concentrated solution presents interesting nonlinear optical properties. This organic material shows an important self-phase modulation with changes bigger than 2π. We present a diffraction ring patterns study of the Hibiscus Sabdariffa solution. Numerical results of transmittance, with refraction and simultaneous absorption, are shown.

  8. Effect of Hibiscus sabdariffa on obesity in MSG mice.

    Alarcon-Aguilar, Francisco J; Zamilpa, Alejandro; Perez-Garcia, Ma Dolores; Almanza-Perez, Julio C; Romero-Nuñez, Eunice; Campos-Sepulveda, Efrain A; Vazquez-Carrillo, Laura I; Roman-Ramos, Ruben

    2007-10-08

    The aim of the present investigation was determine whether a standardized Hibiscus sabdariffa calyces aqueous extract has an effect on body weight in an obese animal model induced by the administration of monosodium glutamate. Hibiscus sabdariffa aqueous extract, containing 33.64 mg of total anthocyanins per each 120 mg of extract, was orally administered (120 mg/kg/day) for 60 days to healthy and obese mice, and body weight gain, food and liquid intake, aspartate aminotransferase (AST), alanine aminotransferase (ALT), cholesterol, and triglycerides levels were measured. Hibiscus sabdariffa administration significantly reduced body weight gain in obese mice and increased liquid intake in healthy and obese mice. ALT levels were significantly increased on the 15th and 45th days in obese mice, but AST levels did not show significant changes. Mortality was not observed in the Hibiscus sabdariffa treated groups. Triglycerides and cholesterol levels showed non-significant reductions in animals treated with Hibiscus sabdariffa. Our data confirm the anti-obesity effect of Hibiscus sabdariffa reported by the Mexican population.

  9. Ekstraksi dan Uji Stabilitas Zat Warna Alami dari Bunga Kembang Sepatu (Hibiscus rosa-sinensis L dan Bunga Rosela (Hibiscus sabdariffa L

    Yusraini Dian Inayati Siregar

    2017-03-01

    Full Text Available Ekstraksi dan Uji Stabilitas Zat Warna Alami dari Bunga Kembang Sepatu (Hibiscus rosa-sinensis L dan Bunga Rosela (Hibiscus sabdariffa L telah dilakukan. Penelitian bertujuan untuk mengekstraksi bunga kembang sepatu dan bunga rosella dengan mencari temperatur dan konsentrasi yang optimum untuk mendapatkan pigmen dari bunga kembang sepatu dan bunga rosella dengan pelarut air dan etanol, selain itu dilakukan juga uji stabilitas zat warna. Analisa kadar zat warna dilakukan dengan metode spektrofotometri. Hasil ekstrasi optimum menggunakan metode maserasi dengan pelarut air  adalah pada temperatur 90°C dan dengan pelarut etanol pada konsentrasi 96 %.Uji stabilitas warna memberikan hasil sebagai berikut: a Kondisi penyimpanan, sinar matahari dan sinar lampu dapat mempengaruhi stabilitas zat warna ekstrak Hibiscus rosa-sinensis L dan Hibiscus sabdariffa L dengan meningkatnya nilai absorbansi pada kedua ekstrak. b Penambahan oksidator, H2O2 dapat mempengaruhi stabilitas zat warna ekstrak Hibiscus rosa-sinensis L dan Hibiscus sabdariffa L dengan perubahan dari ekstrak berwarna menjadi ekstrak tidak berwarna karena menghasilkan turunan asam benzoat. c Nilai pH yang semakin meningkat, dari pH 4 ke pH 5, mempengaruhi stabilitas zat warna ekstrak Hibiscus rosa-sinensis L dan Hibiscus sabdariffa L dengan perubahan ekstrak berwarna menjadi tidak berwarna karena terbentuknya basa kuinodal.   Kata Kunci: Ekstraksi, Hibiscus rosa-sinensis L, Hibiscus sabdariffa L, Spektrofotometri UV-Vis

  10. Abelmoschus esculentus

    Pedro

    2015-02-16

    Doreddula et al., 2014). World ... sense, in order to introduce the market a new plant product, a study on the nutritional quality and ..... For some derivatives of phenolic acids, antioxidant activity has been reported, except for those of ...

  11. Oreochromis esculentus

    Prof. Adipala Ekwamu

    Displacement of the two LVR indigenous tilapiines was .... potential contamination of reaction conditions were ... populations was taken as a single locus. ... Table 1. Microsatellite primer sequences and reaction conditions for 10 loci used for.

  12. Interferência de plantas daninhas na cultura do quiabo Weed interference in okra crop

    J.B. Santos

    2010-06-01

    Full Text Available Objetivou-se com este trabalho avaliar os períodos de interferência das plantas daninhas na cultura do quiabo (Abelmoschus esculentus na região do Médio Vale do Rio Doce, em Minas Gerais. O experimento foi conduzido em campo, entre maio e outubro de 2007. Utilizaram-se sementes do quiabo Santa Cruz-47, semeadas no espaçamento de 0,25 x 1 m. Foram estabelecidos diferentes períodos de controle das plantas daninhas na cultura, variando entre zero e 120 dias após a emergência (DAE. Foram avaliados 12 tratamentos, correspondendo a diferentes períodos de controle das plantas daninhas na cultura: capina após a emergência a partir dos 20, 40, 60, 80 e 100 dias; capina após a emergência até os 20, 40, 60, 80 e 100 dias; além de duas testemunhas com capina, ou não capinadas, ambas por 120 dias. Determinou-se o número de frutos por planta e o rendimento (produtividade, bem como os valores em dias para período anterior à interferência (PAI, período crítico de prevenção da interferência (PCPI e período total de prevenção da interferência (PTPI, considerando 5% de perdas. A partir das espécies encontradas na área experimental, avaliou-se também, em vasos, isoladamente ou em competição com o quiabeiro, a capacidade competitiva das principais plantas daninhas. Com base nos resultados, verificou-se que o PAI estimado foi de 25 DAE, indicando a época de início das capinas. Para o PCPI, o período observado foi de 75 dias, indicando PTPI de 100 DAE. Entre as plantas daninhas presentes, Eleusine indica apresentou maior capacidade competitiva sobre a cultura.An experiment was carried out under field conditions in Médio Vale do Rio Doce-MG, from May to October, 2007, to establish periods of weed interference in Abelmoschus esculentus crop. 'Santa Cruz-47' seeds were sown in a 0.25 x 1.0 m spacing, and weed control times varied from 0 to 120 days after emergence (DAE. Number of fruit per plant and yield as well as values in days

  13. An overview of the HIBISCUS campaign

    Pommereau, J.-P.; Garnier, A.; Held, G.; Gomes, A. M.; Goutail, F.; Durry, G.; Borchi, F.; Hauchecorne, A.; Montoux, N.; Cocquerez, P.; Letrenne, G.; Vial, F.; Hertzog, A.; Legras, B.; Pisso, I.; Pyle, J. A.; Harris, N. R. P.; Jones, R. L.; Robinson, A. D.; Hansford, G.; Eden, L.; Gardiner, T.; Swann, N.; Knudsen, B.; Larsen, N.; Nielsen, J. K.; Christensen, T.; Cairo, F.; Fierli, F.; Pirre, M.; Marécal, V.; Huret, N.; Rivière, E. D.; Coe, H.; Grosvenor, D.; Edvarsen, K.; di Donfrancesco, G.; Ricaud, P.; Berthelier, J.-J.; Godefroy, M.; Seran, E.; Longo, K.; Freitas, S.

    2011-03-01

    The EU HIBISCUS project consisted of a series of field campaigns during the intense convective summers in 2001, 2003 and 2004 in the State of São Paulo in Brazil. Its objective was to investigate the impact of deep convection on the Tropical Tropopause Layer (TTL) and the lower stratosphere by providing a new set of observational data on meteorology, tracers of horizontal and vertical transport, water vapour, clouds, and chemistry in the tropical Upper Troposphere/Lower Stratosphere (UT/LS). This was achieved using short duration research balloons to study local phenomena associated with convection over land, and long-duration balloons circumnavigating the globe to study the contrast between land and oceans. Analyses of observations of short-lived tracers, ozone and ice particles show strong episodic local updraughts of cold air across the lapse rate tropopause up to 18 or 19 km (420-440 K) in the lower stratosphere by overshooting towers. The long duration balloon and satellite measurements reveal a contrast between the composition of the lower stratosphere over land and oceanic areas, suggesting significant global impact of such events. The overshoots are shown to be well captured by non-hydrostatic meso-scale Cloud Resolving Models indicating vertical velocities of 50-60 m s-1 at the top of the Neutral Buoyancy Level (NBL) at around 14 km, but, in contrast, are poorly represented by global Chemistry-Transport Models (CTM) forced by Numerical Weather Forecast Models (NWP) underestimating the overshooting process. Finally, the data collected by the HIBISCUS balloons have allowed a thorough evaluation of temperature NWP analyses and reanalyses, as well as satellite ozone, nitrogen oxide, water vapour and bromine oxide measurements in the tropics.

  14. Responses of growth of lady's fingers (Abelmoschus esculentus L.) to different treatments methods of dairy wastewater.

    Al-Dulaimi, Rana Ibrahim; Ismail, Norli; Ibrahim, Mahamad H

    2014-01-01

    Water is one of the most important precious resources found on the earth, and are most often affected by anthropogenic activities and by industry. Pollution caused by human beings and industries is a serious concern throughout the world. Population growth, massive urbanization, rapid rate of industrialization and modern techniques in agriculture have accelerated water pollution and led to the gradual deterioration of its quality. A large quantity of waste water disposed of at sea or on land has caused environmental problems which have led to environmental pollution, economic losses and chemical risks caused by the wastewater, and its impact on agriculture. However, waste water which contain nutrients and organic matter has possible advantages for agricultural purposes. Therefore, the presented study was undertaken to assess the impact of Dairy Effluent (treated and untreated waste water) on seed germination, seedling growth, dry matter production and the biochemical parameters of lady's fingers (Abelmoschus esculentus L.). A field experiment in a green house was conducted to use raw and treated dairy wastewater for watering lady's fingers (Abelmoschus esculentus L.). The plants were watered using (WW) raw dairy wastewater, (T1) chemicals treatment, (T2) physical treatment, (T3) dilution method treatment and tap water (TW) in pot experiments. Ten plants of each treatment /3 replicate were randomly selected and labelled for the collection of data. The data was collected sequentially, starting with chlorophyll content pre-harvest, vegetative qualities (shoot, root and seedling length) and dry matter quality (shoot and root dry matter) pos-tharvest. The effect was seen on the germination seed and growth of the plant. The results showed inhibitory effect from dairy effluent (WW) on seed germination and plant growth. Treatment with chemicals showed statistically significant differences with other treatments. Chemical treatment (TC2) at 20 mg/L Al2(SO4)3 and pH 6

  15. [Molecular identification of Hibiscus syriacus and its adulterants using ITS2 barcode].

    Liu, Yi-Mei; Jin, Li-Na; Xiong, Yong-Xin; Wu, Lan; Chen, Ke-Li

    2014-03-01

    To identify Hibiscus syriacus and its adulterants using DNA barcoding technique. Nine samples of five species were PCR amplified and sequenced, and twelve samples were downloaded from the GenBank. The intra-specific and interspecific K2P distances were calculated, and neighbor-joining( NJ) tree was constructed by MEGA 5.0. The results showed the intra-specific genetic distances of Hibiscus syriacus were ranged from 0.009 to 0.056, which were far lower than inter-specific genetic distances between Hibiscus syriacus and its adulterants (0.236 - 0.301). Variable sites within Hibiscus syriacus ranged from 2 to 9 which were far less than the adulterants (45 - 52); Different samples of Hibiscus syriacus were gathered together and could be distinguished from its adulterants by NJ tree. ITS2 can discriminate Hibiscus syriacus from its adulterants correctly. The ITS2 region is an efficient barcode for authentication of Hibiscus syriacus and its adulterants.

  16. Preventative and Curative Effects of Several Plant Derived Agents Against Powdery Mildew Disease of Okra

    Moustafa Hemdan Ahmed MOHARAM

    2012-08-01

    Full Text Available The preventative and curative effects of some plant derived agents based on plant extracts or essential oils were studied at different concentrations against Erysiphe cichoracearum DC. ex Merat, the causal pathogen of okra powdery mildew by the detached leaf-disk and potted plants bioassays. Through detached leaf-disk assay, the highest mean preventative effect (97.74% was recorded by neem seed oil followed by jojoba oil (89.82% and extract of Rynoutria sachalinensis (82.77%. Neem seed oil at 1% was the most effective agent followed by jojoba oil and extract of R. sachalinensis at 1.5% and 2%, respectively, where they suppressed E. cichoracearum completely. Potted plants assay revealed that neem seed oil, jojoba oil and extract of R. sachalinensis as well as the fungicide (active ingredient dinocap showed higher preventative efficacy at all leaf olds treated after 7 and 14 days of inoculation as compared with extracts of henna and garlic. Moreover, the preventative efficacy partly remained apparent after 14 days of inoculation at all leaf olds tested. In field trials through 2010 and 2011 growing seasons, when the first symptoms of powdery mildew appeared naturally, 1.5% jojoba oil, 2% extract of R. sachalinensis and 1% neem seed oil were sprayed individually twice on grown plants to evaluate their efficacy on controlling powdery mildew, growth and yield of okra. Resulted showed that neem seed oil was the most effective agent and highly decreased the disease severity to 29.92%, recorded the highly curative effect (68.15% and also improved plant growth and pods yield.

  17. Physicochemical Characteristics and Composition of Three Morphotypes of Cyperus esculentus Tubers and Tuber Oils

    Souleymane Bado

    2015-01-01

    Full Text Available Tuber characteristics and nutrient composition of three morphotypes of Cyperus esculentus tubers and tuber oils were determined. The mean value for length and width of the tuber and one thousand dried tuber weights ranged from 0.98 to 1.31 cm, 0.90 to 1.19 cm, and 598 to 1044 g, respectively. Tubers displayed high level of starch (30.54–33.21 g 100 g−1, lipid (24.91–28.94 g 100 g−1, and sucrose (17.98–20.39 g 100 g−1. The yellow tubers had significantly higher content in lipid compared to black ones. Levels of ascorbic acid, tocopherol, and β-carotene of the three morphotypes differed significantly. Yellow ones (morphotypes 1 and 2 were the richest in tocopherol and the poorest in β-carotene. Saturated fatty acid content of morphotype 2 was significantly lower than that of morphotypes 1 and 3. Morphotype 3 had the significantly lowest PUFA content compared to morphotypes 1 and 2. Morphotype 1 was found to be richer in Ca, Cu, and Mn contents. Al, Mg, P, S, and Si were most abundant in morphotype 2. Morphotype 3 had the highest content of Cl, K, and Zn.

  18. Physicochemical, functional and sensory attributes of milk prepared from irradiated tiger nut (Cyperus esculentus L.

    Abenaa A. Okyere

    2014-10-01

    Full Text Available Five tiger nut (Cyperus esculentus L. cultivars were collected from four different regions of Ghana and irradiated. The aim of this study was to evaluate some physicochemical, functional and sensory qualities of milk produced from irradiated tiger nut samples. Analysis was carried out for pH, total solids, moisture, sugar brix and viscosity. Finally the consumer acceptability of the milk prepared from the nuts was determined by a taste panel using the parameters of colour, taste, aroma, mouth feel and overall acceptability. The sugar content varied from 6.0 ± 0.3% (Techiman to 15.00 ± 1.00% (Asebu Ekroful depending on the irradiation dose applied. Generally, increase in dose increased the sugar availability but decreased viscosity of the milk prepared from the nuts. The milk with the highest viscosity was from Kwahu Aduamoa and Techiman with the least viscosity from Bawjiase. Generally, no significant difference was detected by the sensory panellists with regard to mouth feel and taste among the milk samples prepared from the various tiger nut cultivars.

  19. Identification of DNA Fragments that Showed Linkage to the Radiation-induced Yellow Vein Mosaic Disease Resistance Mutation in Okra

    Boonsirichai, Kanokporn; Phadvibulya, Valailak; Adthalungrong, Amnuai; Srithongchai, Wanphen; Puripunyavanich, Vichai

    2007-08-01

    Full text: The yellow vein mosaic disease resistant mutant of okra was crossed to Pichit 03, a susceptible variety. Their progeny showed prolonged resistance when compared with Pichit 03. DNA fingerprints of F2 and BC1F1 individuals from the cross indicated that most DNA bands did not segregate with either the resistance or the susceptible characteristics. Nonetheless, polymorphic DNA bands could be identified between the mutant and Okura, the parental variety

  20. Biology of Pink Hibiscus MealybugMaconellicoccushirsutus (Green on Chinese Hibiscus Shrubs in Khuzestan Province

    M. Sadat Alizadeh

    2017-12-01

    Full Text Available Introduction: The pink hibiscus mealybug, Maconellicoccushirsutus (Green (Hem.:Pseudococcidae is one of the serious economic pests of agricultural crops in tropical and subtropical regions.This pesthas spread through Asia, Africa, America and Australia, and infests more than 330 host plants which some of them have the important role in theeconomy. This sap-feeding insect secrets honeydew which provides suitablemedium for black sooty moldgrowth. It also releases a toxin during feeding, which results in severe stunting, decline, and deformation of twigs. It has recorded as a pest on different host plants in southern Iranian provinces since the 1990s.In Khuzestan province, it was first observed on Chinese hibiscus shrubs in urban landscapes of Ahvaz in February 2009. The evergreen hibiscus shrub is very common in the urban landscape of warmcities of Khuzestan province. The severity of mealybug damage to hibiscus shrubs causedto becut off in some regions. As there is no data on the biology of M. hirsutus in Iran,in the present study, the biologyof this pestwas investigatedat different temperatures in laboratory conditions as well as its population fluctuations on Chinese hibiscus shrubs in urban landscapes of Ahvaz. Materials and methods: Biology of the pest species was evaluated at five laboratory constant temperatures 15, 20, 25, 30and 35±2°C (65±5% RH and a photoperiod of L: D 14:10 h on Chinese hibiscus leaves in plastic boxes (8×6×2 cm. Number ofM. hirsutusgenerations was also studied in clip cages on hibiscus shrubs in Ahvaz urban landscapes within a year. Population fluctuations of the insect were also investigated on those shrubs in two different regions of Ahvaz for 16 months. For this purpose, random sampling was done on five shrubs in each region bi-weekly. Eight twigs, 5 cm in length, were cut from the upper and lower halves of each shrub quadrant and a number of all mealybug developmental stages per twig were counted and recorded

  1. Interspecific hybridization among cultivars of hardy Hibiscus species section Muenchhusia

    Kuligowska, Katarzyna; Lütken, Henrik Vlk; Christensen, Brian

    2016-01-01

    Rose mallows belong to the Muenchhusia section of the Hibiscus genus. They represent a small group of cold tolerant North American plants and are popular ornamentals mainly because of their abundant, large and colorful flowers. Due to their geographical origin they are well suited for garden use...... in temperate regions worldwide. The aim of the study was to investigate hybridization barriers in crosses among cultivars of Hibiscus species from the Muenchhusia section: H. coccineus, H. laevis and H. moscheutos. Crossing barriers were identified as both pre- and post-zygotic. The analysis of pollen tube...

  2. Application of the exploratory analysis of data in the geographical discrimination of okra of Rio Grande do Norte and Pernambuco

    Francisco Santos Panero

    2009-11-01

    Full Text Available The contents of Cu, Zn, Na, Fe, K, Ca, Mn, Mg, PO43-, Cl- and SO42- were determined in samples of okra of the municipal districts of Caruaru and Vitória de Santo Antão, in Pernambuco, as well as in the municipal districts of Ceará-Mirim, Macaíba and Extremoz in the state of Rio Grande do Norte. The objective of this work is the application of two methods of  exploratory analysis of data: Principal Component Analysis - PCA and Hierarquical Cluster Analysis - HCA in the geographical discrimination of okra originating in the states of Rio Grande do Norte and Pernambuco. The results showed that Cl- and Na were the main elements for the differentiation of the samples of Rio Grande do Norte and, the samples of Pernambuco presented the largest amount of Fe, Cu, Mn, Mg, Ca, Zn, K, PO43-, and SO42-. Boths the methods of exploratory analysis of data investigated are efficient for geographical discrimination of okra originating in Rio Grande do Norte and Pernambuco.

  3. Identification of a disease complex involving a novel monopartite begomovirus with beta- and alphasatellites associated with okra leaf curl disease in Oman.

    Akhtar, Sohail; Khan, Akhtar J; Singh, Achuit S; Briddon, Rob W

    2014-05-01

    Okra leaf curl disease (OLCD) is an important viral disease of okra in tropical and subtropical areas. The disease is caused by begomovirus-satellite complexes. A begomovirus and associated betasatellite and alphasatellite were identified in symptomatic okra plants from Barka, in the Al-Batinah region of Oman. Analysis of the begomovirus sequences showed them to represent a new begomovirus most closely related to cotton leaf curl Gezira virus (CLCuGeV), a begomovirus of African origin. The sequences showed less than 85 % nucleotide sequence identity to CLCuGeV isolates. The name okra leaf curl Oman virus (OLCOMV) is proposed for the new virus. Further analysis revealed that the OLCOMV is a recombinant begomovirus that evolved by the recombination of CLCuGeV isolates with tomato yellow leaf curl virus-Oman (TYLCV-OM). An alpha- and a betasatellite were also identified from the same plant sample, which were also unique when compared to sequences available in the databases. However, although the betasatellite appeared to be of African origin, the alphasatellite was most closely related to alphasatellites originating from South Asia. This is the first report of a begomovirus-satellite complex infecting okra in Oman.

  4. Protective role of aqueous extract of Hibiscus sabdariffa (calyx ...

    USER

    2010-05-24

    May 24, 2010 ... of electron on the electron transport chain. There is a dose-dependent relationship between lipid peroxidation induced by potassium bromate and the protection offered by the extract of H. sabdariffa. REFERENCES. Alli MB, Salih M (1991). Investigation of the antispasmodic potential of. Hibiscus sabdariffa ...

  5. Chemical evaluation of three varieties of Roselle ( Hibiscus ...

    Chemical evaluation of three varieties of Roselle ( Hibiscus Sabdariffa L.) seeds. SO Babalola, OC Oworh, KO Alasiri, JA Adetunji. Abstract. No Abstract. Nigerian Journal of Horticultural Science Vol. 11 () 2006: pp.31-35. Full Text: EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT.

  6. Haematological effects of aqueous extract of Hibiscus Sabdariffa

    The present study was undertaken to investigate the effects of aqueous extract of Hibiscus sabdariffa petals on some haematological parameters. The extract was administered orally in doses of 0.5, 1.0 and 1.5mg/kg body weight to normal Wistar rats for 21 days. The results showed that the extract decreased significantly (p ...

  7. Estimation of Planting Dates for Roselle ( Hibiscus sabdariffa L.) in ...

    In order to fill the gap existing between the production and consumption of roselle in the humid tropical areas of Nigeria, this experiment was carried out in Owerri between May and December, 2012 to evaluate the effects of planting dates and accessions on the growth and performance of roselle (Hibiscus sabdariffa).

  8. Effects of aqueous extract of Hibiscus sabdariffa calyces on ...

    The effect of aqueous extract of Hibiscus sabdariffa calyces on the haematological profile of normal male albino rats was investigated for 28 days using standard methods. The rats were divided into five groups comprising of the control group, I; which received equal volume of distilled water and four treatment groups, II, III, ...

  9. Oppression and Revolt in Chimamanda Adichie's Purple Hibiscus ...

    This paper examines the oppressive practice of Chimamanda Adichie's character – Eugene in Purple Hibiscus on members of his nuclear family. This oppression endangers normal familial relationship as the oppressed view their oppression as an infringement on their fundamental human rights. Therefore, they express ...

  10. Haematinic activity of Hibiscus Cannabinus | Agbor | African Journal ...

    The haematinic activity of an orally administered aqueous extract of Hibiscus cannabinus leaves was studied on haemolytic anaemic rats. Anaemia was induced by an oral administration of phenylhydrazine for a period of 8 days. Red blood cell count, haemoglobin concentration, and pack cell volume were analysed as ...

  11. Seed trichome variation in Hibiscus section Furcaria in East Africa ...

    The micromorphology of the seed trichomes in Hibiscus section Furcaria in Tropical East Africa was surveyed. The structure of the trichomes was found to be unique for each species examined, where such trichomes are present. Seed trichomes of specimens of H. altissimus Horny from the Kenya and Tanzania coasts were ...

  12. Free radical scavenging activities of pigment extract from Hibiscus ...

    The macroporous resin adsorption method was used to purify the pigment from Hibiscus syriacus L. petals. Through the comparison of the adsorption and desorption rates of six types of macroporous resins including AB-8, S-8, NKA-9, DM-130, D101 and 860021 to the pigment, 860021 resin was selected as the most ...

  13. Effect of aqueous extracts of Hibiscus sabdariffa and Zingiber ...

    Effect of aqueous extracts of Hibiscus sabdariffa and Zingiber Officinale on blood cholesterol and glucose levels of rats. ... Subsequently, group 3 rats were given 0.8 ml/kg body weight of H. sabdarffa extract, while those in group 4 were given 0.2 ml Z. officinale extract/kg body weight. Rats in group 5 were given 1 ml of H.

  14. Domestic violence in Chimamanda Ngozi Adichie's Purple Hibiscus ...

    Gender-based violence is an issue that has become a part of modern society, cutting across cultures, race, ethnicity and status. In Adichie's Purple Hibiscus, gender-based violence manifests in the form of domestic violence, projected through the Eugene Achike family around whom the story, set in the eastern part of ...

  15. Isolation and Characterization of Anthocyanins from Hibiscus sabdariffa Flowers.

    Grajeda-Iglesias, Claudia; Figueroa-Espinoza, Maria C; Barouh, Nathalie; Baréa, Bruno; Fernandes, Ana; de Freitas, Victor; Salas, Erika

    2016-07-22

    The intense red-colored Hibiscus sabdariffa flowers are an inexpensive source of anthocyanins with potential to be used as natural, innocuous, and health-beneficial colorants. An anthocyanin-rich extract from hibiscus flowers was obtained by ultrasound-assisted extraction. By a single-step process fractionation using a Sep-Pak C18 cartridge, the main hibiscus anthocyanins, delphinidin-3-O-sambubioside (Dp-samb) and cyanidin-3-O-sambubioside (Cy-samb), were separated and then characterized via NMR and HPLC-ESIMS data. Since Dp-samb was the most abundant anthocyanin identified in the extract, its colorant properties were studied by the pH jumps method, which allowed the calculation of the single acid-base equilibrium (pK'a 2.92), the acidity (pKa 3.70), and the hydration constants (pKh 3.02). Moreover, by using size-exclusion chromatography, new cyanidin-derived anthocyanins (with three or more sugar units) were successfully identified and reported for the first time in the hibiscus extract.

  16. Studies on the constituents of hibiscus rosa-sinensis

    Hossain, M.A.; Tarafdar, S.A.

    2003-01-01

    A new flavone derivative, furano flavone (1) was isolated along with ovalichalcone (2) from the leaves and stems of Hibiscus rosa-sinensis (Malvaceae). The structure of furanoflavone (1) was identified as 5-methoxy-3' methyl-3', 4'-methylenedioxyfurano(2,3; 7,8) flavone by spectroscopic and chemical analysis. (author)

  17. Membrane Stabilizing Activity And Phytochemistry Of Hibiscus rosa ...

    The human erythrocyte membrane stabilizing activity of saline extract of Hibiscus rosa-sinensis leaves was investigated as part of efforts at validating its use as anti-arthritic and anti-inflammatory agent. The results of the membrane stabilizing activity of the extract, when compared to two non-steroidal anti-inflammatory drugs ...

  18. Adichie's Purple Hibiscus and issues of ideology in the constitution ...

    ... as subjects, with the eventual emergence of Kambili to self-knowledge and condition of social responsibility. The aim of this is to examine how Adichie's Purple Hibiscus is typical of how the Nigerian novel engages itself in issues of ideology and how these issues, in turn, crystallize the challenges of nation-ness in Nigeria.

  19. Anthocyanin content of two Hibiscus sabdariffa cultivars grown in ...

    Anthocyanin content of Hibiscus sabdariffa calyces was determined to compare two cultivars from Senegal called Koor and Vimto. Results showed a significant difference in terms of total anthocyanin content (TA) and relative abundance (RA) of anthocyanin species. Values of TA for Vimto were 3-fold higher than Koor's.

  20. Protective influence of Hibiscus sabdariffa , an edible medicinal ...

    The present study was undertaken to examine the protective influence of the alcoholic leaf extract of Hibiscus sabdariffa (Linn) Malvaceae (an indigenous edible medicinal plant used in Ayurvedic and traditional Medicine in India, China and Thailand) on oxidative stress during ammonium chloride induced ...

  1. The effect of aqueous extracts of Hibiscus sabdariffa (Sorrel) calyces ...

    The effect of aqueous extracts of Hibiscus sabdariffa (Sorrel) calyces on heamatological profile and organ pathological changes in Trypanasoma congolense – infected ... Consumption of the extract significantly (p<0.01) retarded the rate of weight gain in both healthy and infected rats; even though the feed-intake was not ...

  2. Effect of Hibiscus sabdariffa on Blood Pressure and Electrolyte ...

    Effect of Hibiscus sabdariffa on Blood Pressure and Electrolyte Profile of Mild to Moderate Hypertensive Nigerians: A Comparative Study with Hydrochlorothiazide. ... Aim: The aim of this study is to investigate the effect of HS consumption on blood pressure (BP) and electrolytes of mild to moderate hypertensive Nigerians ...

  3. The Aqueous Calyx Extract of Hibiscus sabdariffa Lowers Blood ...

    Summary: The antihypertensive effect of Hibiscus sabdariffa (HS) has been validated in animals and man. This study tested the hypothesis that its hypotensive effect may be sympathetically mediated. The cold pressor test (CPT) and handgrip exercise (HGE) were performed in 20 healthy subjects before and after the oral ...

  4. Comparative water relation of three varieties of Hibiscus cannabinus ...

    PROMOTING ACCESS TO AFRICAN RESEARCH ... Kenaf (Hibiscus cannabinus L.) is an industrial fiber crop that is being grown ... Throughout a period of 120 days, parameters of gas exchange characteristics were measured within four regular intervals of 30 days. ... EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT

  5. Anthocyanin content of two hibiscus sabdariffa cultivars grown

    Sukwattanasinit T., Burana-osot J., Sotanaphun. U. 2007. Spectrophotometric Method for. Quantitative Determination of Total. Anthocyanins and Quality Characteristics of Roselle (Hibiscus sabdariffa). Planta Med. 73 (14) : 1517 - 1522. Torres-Morán M.I., Escoto-Delgadillo M., Ron-Parra. J., Parra-Tovar G., Mena-Munguía ...

  6. Production of red wine from roselle (Hibiscus sabdariffa) and ...

    Red wine was produced from must formulated by mixing 10% (w/v) roselle calyces (Hibiscus sabdriffa var. sabdariffa) Hot water extract with pawpaw juice at the rate of 3:1. The must was formulated using a pure strain of Sacchromyces cerevisiae isolated from palm wine, propagated and pitched at a rate of 8% (v/v).

  7. Microbial contaminants of cultured Hibiscus cannabinus and Telfaria ...

    Nine microbial contaminants comprising of five bacteria and four fungi species were isolated from Hibiscus cannabinus and Telfaria occidentalis cultured tissues. The rate of occurrence of bacteria isolates was higher than that of fungi. The bacterial isolates includes Pseudomonas syringae pv phaseolicoli, Bacillus ...

  8. Comparative Antioxidant Activity of Hibiscus sabdariffa and Ascorbic ...

    Lipid peroxidation is becoming a popular biological marker of oxidative stress. Hibiscus sabdariffa has been reported to serve as a herbal remedy for various disease conditions, but studies on its antioxidant activity and the extent to which it acts remain scarce. The antioxidant activity of H. sabdariffa aqueous extracts, ...

  9. Effect of aqueous extract of Hibiscus sabdariffa Calyces on anaemia ...

    The effect of aqueous extract of Hibiscus sabdariffa Calyces on anaemia-induced and normal wistar albino rats were investigated using standard protocols. The haematological parameters which include haemoglobin concentration, packed cell volume, erythrocyte sedimentation rate, Red Blood Cell count and White Blood ...

  10. Changes in the quality of zobo beverages produced from Hibiscus ...

    Quality changes in zobo beverage produced from Hibiscus sabdarifa during storage and the effects of extracts of ginger (Zingiber officinale) alone or in combination with refrigeration at 6C were evaluated for six (6) weeks duration. Results shows that the total viable bacterial count increased from 0.9 ± 0.01 log10 cfu/ml to ...

  11. Fermentation potentials of Citrus limon and Hibiscus sabdariffa juice ...

    This study aimed to determine the fermentation potentials of yeast isolates from Citrus limon and Hibiscus sabdariffa for the fermentation of juice extracts of C. limon and H. sabdariffa. Isolation and morphological studies of yeast cells were carried out by standard protocols. Fourier Transform Infra-red (FT-IR) ...

  12. estimation of planting dates for roselle (hibiscus sabdariffa l.)

    thinkexploitsint'l

    2011-06-11

    Jun 11, 2011 ... 2012 to evaluate the effects of planting dates and accessions on the growth and performance of roselle (Hibiscus ... vegetables in soup making, green calyx roselle should be sown in May and harvested not later ... Roselle is native to the region that stretched from India to Malaysia and almost all parts of.

  13. Physicochemical, functional and pasting properties of flour produced from gamma irradiated tiger nut (Cyperus esculentus L.)

    Ocloo, Fidelis C.K.; Okyere, Abenaa A.; Asare, Isaac K.

    2014-01-01

    Tiger nut (Cyperus esculentus L.) has been recognised as one of the best nutritional crops that can be used to augment the Ghanaian diet. The application of gamma irradiation as means of preserving tiger nut could modify the characteristics of resultant flour. The purpose of this study was to determine the physicochemical, functional and pasting characteristics of flour from gamma irradiated tiger nut. The yellow and black types of tiger nut were sorted, washed and dried in an air-oven at 60 o C for 24 h. The dried tiger nut samples were irradiated at 0.0, 2.5, 5.0 and 10.0 kGy and then flours produced from them. Moisture, ash, pH, titratable acidity, water and oil absorption capacities, swelling power, solubility, bulk density and pasting properties of the flours were determined using appropriate analytical methods. Results showed that irradiation did not significantly (P>0.05) affect the moisture and ash contents of the resultant flours. Gamma irradiation significantly (P≤0.05) increased titratable acidity with concomitant decrease in pH of the flours. No significant differences were observed for water and oil absorption capacities, swelling power as well as bulk density. Solubility significantly (P≤0.05) increased generally with irradiation dose. Peak viscosity, viscosities at 92 °C and 55 °C, breakdown and setback viscosities decreased significantly with irradiation dose. Flour produced from irradiated tiger nut has a potential in complementary food formulations due to its low viscosity and increased solubility values. - Highlights: • Physicochemical, functional and pasting characteristics of flour from gamma irradiated tiger nut were studied. • Irradiation did not affect the moisture and ash contents of the resultant flours. • Titratable acidity increased with decrease in pH of the flours from the irradiated tiger nut. • Solubility increased whereas peak viscosity decreased with irradiation dose. • Flour produced from irradiated tiger nut has a

  14. KINETICS OF MODULATORY ROLE OF Cyperus esculentus L. ON THE SPECIFIC ACTIVITY OF KEY CARBOHYDRATE METABOLIZING ENZYMES.

    Sabiu, Saheed; Ajani, Emmanuel Oladipo; Sunmonu, Taofik Olatunde; Ashafa, Anofi Omotayo Tom

    2017-01-01

    The continuous search for new lead compounds as viable inhibitors of specific enzymes linked to carbohydrate metabolism has intensified. Cyperus esculentus L. is one of the therapeutically implicated botanicals against several degenerative diseases including diabetes mellitus. This study evaluated the antioxidant and mechanism(s) of inhibitory potential of aqueous extract of C. esculentus on α-amylase and α-glucosidase in vitro . The extract was investigated for its radical scavenging and hypoglycaemic potentials using standard experimental procedures. Lineweaver-Burke plot was used to predict the manner in which the enzymes were inhibited. The data obtained revealed that the extract moderately and potently inhibited the specific activities of α -amylase and α -glucosidase, respectively. The inhibition was concentration-related with respective IC 50 values of 5.19 and 0.78 mg/mL relative to that of the control (3.72 and 3.55 mg/mL). The extract also significantly scavenged free radicals and the effects elicited could be ascribed to its phytoconstituents. The respective competitive and non-competitive mode of action of the extract is due to its inhibitory potentials on the activities of α -amylase and α -glucosidase. Going forward, in addition to completely characterize the exact compound(s) responsible for the elicited activity in this study, pertinent attention will be given to the in vivo evaluation of the identified constituents.

  15. Potential of Soil Amendments (Biochar and Gypsum in increasing Water Use Efficiency of Abelmoschus esculentus L. Moench

    Aniqa eBatool

    2015-09-01

    Full Text Available Water being an essential component for plant growth and development, its scarcity poses serious threat to crops around the world. Climate changes and global warming are increasing the temperature of earth hence becoming an ultimate cause of water scarcity. It is need of the day to use potential soil amendments that could increase the plants’ resistance under such situations. Biochar and gypsum were used in the present study to improve the water use efficiency and growth of Abelmoschus esculentus L. Moench (Lady’s Finger. A six weeks experiment was conducted under greenhouse conditions. Stress treatments were applied after thirty days of sowing. Plant height, leaf area, photosynthesis, transpiration rate, stomatal conductance and water use efficiency were determined weekly under stressed (60% field capacity and non-stressed (100% field capacity conditions. Stomatal conductance and transpiration rate decreased and reached near to zero in stressed plants. Stressed plants also showed resistance to water stress upto five weeks and gradually perished at sixth week. On the other hand, water use efficiency improved in stressed plants containing biochar and gypsum as compared to untreated plants. Biochar alone is a better strategy to promote plant growth and WUE specifically of Abelmoschus esculentus, compared to its application in combination with gypsum.

  16. Relationships between anthocyanins and other compounds and sensory acceptability of Hibiscus drinks.

    Bechoff, Aurélie; Cissé, Mady; Fliedel, Geneviève; Declemy, Anne-Laure; Ayessou, Nicolas; Akissoe, Noel; Touré, Cheikh; Bennett, Ben; Pintado, Manuela; Pallet, Dominique; Tomlins, Keith I

    2014-04-01

    Chemical composition of Hibiscus drinks (Koor and Vimto varieties, commercial and traditional, infusions and syrups) (n=8) was related to sensory evaluation and acceptance. Significant correlations between chemical composition and sensory perception of drinks were found (i.e. anthocyanin content and Hibiscus taste) (pHibiscus drink and anthocyanin content. The study showed that the distinctions between the acceptability groups are very clear with respect to the chemical composition and rating of sensory attributes. Copyright © 2013 Elsevier Ltd. All rights reserved.

  17. Degradation analysis of some synthetic and bio-insecticides sprayed on okra crop using HPLC

    Abar, M.F.; Haq, M.A.; Yasmin, N.; Khan, M.F.U.

    2012-01-01

    This study aimed to find out the degradation of three conventional and two bio-insecticides sprayed on okra crop. Imidacloprid, Endosulfan and Profenofos were selected as convectional and biosal and spinosad as bioinsecticide. The insecticides were sprayed at the rates of 49.4, 642.2, 988, 35.5 and 158 g. a. i. ha/sup -1/ respectively. The insecticide residues were analyzed in the leaf and fruit after 0, 1, 3 and 7 days using high performance liquid chromatography. First order degradation kinetics was fitted on this data and degradation rate constants and half life were calculated. Conventional insecticides were found to be more persistent in the crop (Average half life: 1.95, 2.42 and 1.57 days for imidacloprid, endosulfan and profenofos respectively) than bioinsecticides (Average half life 1.25 and 0.27 days for spinosad and biosal respectively). Residues of all tested insecticides were compared with codex and EU MRLs and found both the bio-insecticides treated crops safe for human consumption even after few hours of spray. Endosulfan and profenofos treated crops were not found to be fit for consumption even after 7 days of application. Imidacloprid being biorational (low risk) was also safe for consumption on the next day of application. (author)

  18. Effect of okra cell wall and polysaccharide on physical properties and stability of ice cream.

    Yuennan, Pilapa; Sajjaanantakul, Tanaboon; Goff, H Douglas

    2014-08-01

    Stabilizers are used in ice cream to increase mix viscosity, promote smooth texture, and improve frozen stability. In this study, the effects of varying concentrations (0.00%, 0.15%, 0.30%, and 0.45%) of okra cell wall (OKW) and its corresponding water-soluble polysaccharide (OKP) on the physical characteristics of ice cream were determined. Ice cream mix viscosity was measured as well as overrun, meltdown, and consumer acceptability. Ice recrystallization was determined after ice cream was subjected to temperature cycling in the range of -10 to -20 °C for 10 cycles. Mix viscosity increased significantly as the concentrations of OKW and OKP increased. The addition of either OKW or OKP at 0.15% to 0.45% significantly improved the melting resistance of ice cream. OKW and OKP at 0.15% did not affect sensory perception score for flavor, texture, and overall liking of the ice cream. OKW and OKP (0.15%) reduced ice crystal growth to 107% and 87%, respectively, as compared to 132% for the control (0.00%). Thus, our results suggested the potential use of OKW and OKP at 0.15% as a stabilizer to control ice cream quality and retard ice recrystallization. OKP, however, at 0.15% exhibited greater effect on viscosity increase and on ice recrystallization inhibition than OKW. © 2014 Institute of Food Technologists®

  19. Effects of different traditional cooking methods on nutrients and ...

    The objective of this research was to determine the effect of cooking using two different methods of preparing okra soup in Ondo state on nutrient, mineral content including zinc bioavailability of okra, Abelmoschus esculentus. The okra fruits were grated and divided into four lots; two lots were cooked with other ingredients of ...

  20. The nutraceutical benefits of subfractions of Abelmoschus esculentus in treating type 2 diabetes mellitus.

    Chien-Ning Huang

    Full Text Available Abelmoschus esculentus (AE, a commonly consumed vegetable, is well-known for its anti-hyperglycemic effects. However, few scientific reports have identified its targets because mucilage increases the difficulty of manipulation. We recently reported extraction steps to obtain subfractions of AE, which were found to attenuate the adverse effects of high glucose and fatty acid in vitro. In this study, we used modified extraction steps and type 2 diabetic rats to explore whether AE subfractions can improve the metabolic disturbances caused by insulin resistance in vivo. AE subfractions (F1, F2, and FR were prepared. The type 2 diabetes model was induced by feeding male Sprague-Dawley rats with a high-fat diet and injecting them with 35 mg/kgbw streptozotocin when their body weight reached 475 ± 15 g. After a hyperglycemic status had been confirmed, the rats were tube-fed with or without different doses of AE subfractions. Serum glucose, lipid markers, insulin, HbA1c and HOMA-IR were measured in the following 12 weeks. Serum glucose promptly increased and insulin resistance was noted in the diabetic rats (glucose: 360-500 mg/dl, HOMA-IR 9.8-13.8. F2, rich in polysaccharides and carbohydrates, was most effective in attenuating hyperglycemia and insulin resistance (glucose: 200 mg/dl; HOMA-IR: 5.3 and especially HbA1C (from 8.0% to 6.5%. All of the AE subfractions lowered the level of triglycerides and free fatty acid, but not the level of total cholesterol. FR significantly increased the high-density lipoprotein/low-density lipoprotein ratio, indicating its benefits for lipoprotein profiles. While F2 and FR were associated with weight gain, F1 possessed an anti-obese effect. In conclusion, whether it is consumed as a vegetable or as a nutraceutical, AE has the potential to be an adjuvant therapy for diabetes. AE subfractions could be developed individually and deserve further investigation.

  1. Genomic effects on advertisement call structure in diploid and triploid hybrid waterfrogs (Anura, Pelophylax esculentus).

    Hoffmann, Alexandra; Reyer, Heinz-Ulrich

    2013-12-04

    In anurans, differences in male mating calls have intensively been studied with respect to taxonomic classification, phylogeographic comparisons among different populations and sexual selection. Although overall successful, there is often much unexplained variation in these studies. Potential causes for such variation include differences among genotypes and breeding systems, as well as differences between populations. We investigated how these three factors affect call properties in male water frogs of Pelophylax lessonae (genotype LL), P. ridibundus (RR) and their interspecific hybrid P. esculentus which comes in diploid (LR) and triploid types (LLR, LRR). We investigated five call parameters that all showed a genomic dosage effect, i.e. they either decreased or increased with the L/R ratio in the order LL-LLR-LR-LRR-RR. Not all parameters differentiated equally well between the five genotypes, but combined they provided a good separation. Two of the five call parameters were also affected by the breeding system. Calls of diploid LR males varied, depending on whether these males mated with one or both of the parental species (diploid systems) or triploid hybrids (mixed ploidy systems). With the exception of the northernmost mixed-ploidy population, call differences were not related to the geographic location of the population and they were not correlated with genetic distances in the R and L genomes. We found an influence of all three tested factors on call parameters, with the effect size decreasing from genotype through breeding system to geographic location of the population. Overall, results were in line with predictions from a dosage effect in L/R ratios, but in three call parameters all three hybrid types were more similar to one or the other parental species. Also calls of diploid hybrids varied between breeding systems in agreement with the sexual host required for successful reproduction. The lack of hybrid call differences in a mixed-ploidy population at

  2. Comparison of Antioxidative Activity among Different Types of Hibiscus

    Uezu, Eiko; Fu, Churan; Kyan, Chie; Nago, Chihiro; 上江洲, 榮子; 付, 楚然; 喜屋武,千 恵; 名護, ちひろ

    2013-01-01

    Increased longevity among the population of Okinawa has led to considerable international interest in the role that the local diet and custom might play in this phenomenon. The research has indicated that flowers used for the food have potential health benefit. To explore their value as functional foods, the antioxidative properties of Hibiscus rosa-sinensis, which is the most common flower in Okinawa, was evaluated. The antioxidative activity of three types of the flower-red, orange, and yel...

  3. Hibiscus sabdariffa L., roselle calyx, from ethnobotany to pharmacology.

    Carvajal-Zarrabal, Octavio; Barradas-Dermitz, Dulce María; Orta-Flores, Zaida; Hayward-Jones, Patricia Margaret; Nolasco-Hipólito, Cirilo; Aguilar-Uscanga, M Guadalupe; Miranda-Medina, Anilú; Bujang, Kopli Bin

    2012-01-01

    Using MEDLINE and SCOPUS databases, a review of the literature from the pioneering study of 1991 until 2010 was performed on the effects on biological models of Hibiscus sabdariffa L. roselle calyx, its extracts mainly in polar solvents, or pure components found in extracts, as well as their possible relationship to these effects. Three relevant effects on lipid metabolism, antihypertensive activity, and apoptosis were observed. Our chronological review of the studies mentioned in the literature provides another opportunity to see how humans compile scientific knowledge of a chemical structure-physiological activity relationship starting from an ethnobotanical-ethnopharmagognosy contribution. The chemical components that are the main active principles in the physiological activities of Hibiscus sabdariffa L. calyx are anthocyanins and polyphenols (protocatechuic acid and quercetin). Advances have also been made in the elucidation of action mechanisms. Additionally, it has become clear that the lack of standardization in terms of chemical components of the material arising from Hibiscus sabdariffa L. used in testing on biological models imposes limits on the possibility of carrying out comparative analyses between studies. Fortunately, more recent studies are overcoming this obstacle by reporting component concentrations of assumed active principles; however, complete analysis of the extract, if this is to be considered as a therapeutic agent, is not commonly reported in the aforesaid studies. If one of the eventual scenarios for Hibiscus sabdariffa L. calyx is as a therapeutic agent in communities with economic limitations, then studies of a pharmacological nature should guarantee the effectiveness, safety, and tolerability of this material, which is widely accepted to be associated with chemical complexity, thus making this knowledge necessary.

  4. Hibiscus sabdariffa L., roselle calyx, from ethnobotany to pharmacology

    Carvajal-Zarrabal, Octavio; Barradas-Dermitz, Dulce María; Orta-Flores, Zaida; Hayward-Jones, Patricia Margaret; Nolasco-Hipólito, Cirilo; Aguilar-Uscanga, M Guadalupe; Miranda-Medina, Anilú; Bujang, Kopli Bin

    2012-01-01

    Using MEDLINE and SCOPUS databases, a review of the literature from the pioneering study of 1991 until 2010 was performed on the effects on biological models of Hibiscus sabdariffa L. roselle calyx, its extracts mainly in polar solvents, or pure components found in extracts, as well as their possible relationship to these effects. Three relevant effects on lipid metabolism, antihypertensive activity, and apoptosis were observed. Our chronological review of the studies mentioned in the literature provides another opportunity to see how humans compile scientific knowledge of a chemical structure–physiological activity relationship starting from an ethnobotanical–ethnopharmagognosy contribution. The chemical components that are the main active principles in the physiological activities of Hibiscus sabdariffa L. calyx are anthocyanins and polyphenols (protocatechuic acid and quercetin). Advances have also been made in the elucidation of action mechanisms. Additionally, it has become clear that the lack of standardization in terms of chemical components of the material arising from Hibiscus sabdariffa L. used in testing on biological models imposes limits on the possibility of carrying out comparative analyses between studies. Fortunately, more recent studies are overcoming this obstacle by reporting component concentrations of assumed active principles; however, complete analysis of the extract, if this is to be considered as a therapeutic agent, is not commonly reported in the aforesaid studies. If one of the eventual scenarios for Hibiscus sabdariffa L. calyx is as a therapeutic agent in communities with economic limitations, then studies of a pharmacological nature should guarantee the effectiveness, safety, and tolerability of this material, which is widely accepted to be associated with chemical complexity, thus making this knowledge necessary. PMID:27186114

  5. Pengaruh Periode Panen Terhadap Viabilitas Benih Rosela (Hibiscus sabdariffa L.)

    Pulungan, Dedes Mayang Sari

    2016-01-01

    Rosella has a harvest period that does not simultaneously or concurrently so that harvesting done gradually. This research aimed to determine the effect of harvest on seed viability period of roselle (Hibiscus sabdariffa L.).Conducted in the experimental field of the Faculty of Agriculture, University of North Sumatra which were ± 25 meters above sea level and continued in Seed Technology Laboratory of the Faculty of Agriculture, University of North Sumatra from February to June 2013, using r...

  6. Kombucha Rosella (Hibiscus Sabdariffa Linn) dan Kemampuannya sebagai Antihiperkolesterolemia

    Suhartatik, Nanik; Karyantina, Merkuria; Purwanti, Indrias Tri

    2009-01-01

    Kita mengenal adanya teh yang diekstrak dari kelopak bunga rosella (Hibiscus sabdariffa Linn). Teh jenis ini dapat dibuat menjadi kombucha dengan cara memfermentasikan ekstrak rosella menggunakan peran mikrobia dalam fer­ mentasi kombucha. Mikrobia dalam fermentasi kombucha ini ditumbuhkan dalam ekstrak rosella sebagai medium per­ tumbuhan dengan variasi konsentrasi rosella (30, 40, 50 gram rosella kering/L). Selama proses fermentasi, kombucha rosella dianalisis pH, total asam, dan aktivitas ...

  7. In vitro inhibitory activities of the extract of Hibiscus sabdariffa L ...

    In vitro inhibitory activities of the extract of Hibiscus sabdariffa L. (family malvaceae) on selected cytochrome p450 isoforms. SS Johnson, FT Oyelola, T Ari, H Juho. Abstract. Literature is scanty on the interaction potential of Hibiscus sabdariffa L., plant extract with other drugs and the affected targets. This study was ...

  8. Two New Disease-Resistant Hibiscus: 'Lufkin Red' and 'Lufkin White'

    The Agricultural Research Service, United States Department of Agriculture, has released Hibiscus laevis ‘Lufkin Red’ and ‘Lufkin White’, two new hardy native ornamental hibiscus. These cultivars are recommended for trial by gardeners and horticulturists as summer flowering perennial landscape pl...

  9. Investigation on the phytosanitary status of major ornamental hibiscus species in Italy to assess virus infection

    The genus Hibiscus (family Malvaceae) includes more than 250 species that vary from annual to perennial herbs, and shrubs to small trees that are native to tropical, sub-tropical and temperate climates. A study in 2010-2011 examined viruses associated with symptoms observed on hibiscus plants in It...

  10. Influence of seaweed liquid fertilizer of ulva lactuca on the seed germination, growth, productivity of Abelmoschus esculentus (L.

    Kaisarla Divya

    2015-12-01

    Full Text Available The present investigation is an attempt to study the influence of seaweed liquid fertiliger (SLF extracted from the marine green algae ulva lactuca on the growth parameters of Abelmoschus esculentus (l medikus. The seeds were soaked in different concentrations of SLF viz., 2.5%, 5%, 10%, 20% and control for 6h. The foliar spry was applied twice at four concentrations of seaweed extract. The maximum growth was recorded at 5% concentration viz., seed germination, shoot length, root length, no. of flowers, no. of fruits, fresh weight and dry weight when compare to control and remaining concentrations of seaweed extract. The seaweed extract are found effective in increasing the growth parameters.

  11. Ekstraksi Dan Uji Stabilitas Zat Warna Alami Dari Bunga Kembang Sepatu (Hibiscus Rosa-sinensis L) Dan Bunga Rosela (Hibiscus Sabdariffa L)

    Siregar, Yusraini Dian Inayati; Nurlela, Nurlela

    2011-01-01

    Ekstraksi dan Uji Stabilitas Zat Warna Alami dari Bunga Kembang Sepatu (Hibiscus rosa-sinensis L) dan Bunga Rosela (Hibiscus sabdariffa L) telah dilakukan. Penelitian bertujuan untuk mengekstraksi bunga kembang sepatu dan bunga rosella dengan mencari temperatur dan konsentrasi yang optimum untuk mendapatkan pigmen dari bunga kembang sepatu dan bunga rosella dengan pelarut air dan etanol, selain itu dilakukan juga uji stabilitas zat warna. Analisa kadar zat warna dilakukan dengan metode spektr...

  12. STUDIES ON HIBISCUS CANNABINUS, HIBISCUS SABDARIFFA, AND CANNABINUS SATIVA PULP TO BE A SUBSTITUTE FOR SOFTWOOD PULP- PART 1: AS-AQ DELIGNIFICATION PROCESS

    Dharm Dutt; J. S. Upadhyaya; C. H. Tyagi

    2010-01-01

    Hibiscus cannabinus, Hibiscus sabdariffa, and Cannabinus sativa, which are renewable non-woody fiber resources having characteristics similar to that of softwood (bast fibers), when used together with hardwood (core fibers), gave higher pulp yield with good mechanical strength properties when using an alkaline sulphite-anthraquinone (AS-AQ) pulping process rather than a conventional kraft pulping process and bleached more readily than kraft and soda pulps with a CEHH bleaching sequence. A com...

  13. Physicochemical and phytochemical properties of cold and hot water extraction from Hibiscus sabdariffa.

    Ramirez-Rodrigues, Milena M; Plaza, Maria L; Azeredo, Alberto; Balaban, Murat O; Marshall, Maurice R

    2011-04-01

    Hibiscus cold (25 °C) and hot (90 °C) water extracts were prepared in various time-temperature combinations to determine equivalent extraction conditions regarding their physicochemical and phytochemical properties. Equivalent anthocyanins concentration was obtained at 25 °C for 240 min and 90 °C for 16 min. Total phenolics were better extracted with hot water that also resulted in a higher antioxidant capacity in these extracts. Similar polyphenolic profiles were observed between fresh and dried hibiscus extracts. Hibiscus acid and 2 derivatives were found in all extracts. Hydroxybenzoic acids, caffeoylquinic acids, flavonols, and anthocyanins constituted the polyphenolic compounds identified in hibiscus extracts. Two major anthocyanins were found in both cold and hot extracts: delphynidin-3-sambubioside and cyanidin-3-sambubioside. In general, both cold and hot extractions yielded similar phytochemical properties; however, under cold extraction, color degradation was significantly lower and extraction times were 15-fold longer. Hibiscus beverages are prepared from fresh or dried calyces by a hot extraction and pasteurized, which can change organoleptic, nutritional, and color attributes. Nonthermal technologies such as dense phase carbon dioxide may maintain their fresh-like color, flavor, and nutrients. This research compares the physicochemical and phytochemical changes resulting from a cold and hot extraction of fresh and dried hibiscus calyces and adds to the knowledge of work done on color, quality attributes, and antioxidant capacity of unique tropical products. In addition, the research shows how these changes could lead to alternative nonthermal processes for hibiscus.

  14. Population dynamics of plant nematodes in cultivated soil: length of rotation in newly cleared and old agricultural land.

    Good, J M; Murphy, W S; Brodie, B B

    1973-04-01

    During a 6-year study of 1-, 2-, and 3-year crop rotations, population densities of Pratylenchus brachyurus, Trichodorus christiei, and Meloidogyne incognita were significantly affected by the choice of crops but not by length of crop rotation. The density of P. brachyurus and T. christiei increased rapidly on milo (Sorghum vulgate). In addition, populations of P. brachyurus increased significantly in cropping systems that involved crotalaria (C. rnucronata), millet (Setaria italica), and sudangrass (Sorghum sudanense). Lowest numbers of P. brachyurus occurred where okra (Hibiscus esculentus) was grown or where land was fallow. The largest increase in populations of T. christiei occurred in cropping systems that involved millet, sudangrass, and okra whereas the smallest increase occurred in cropping systems that involved crotalaria or fallow. A winter cover of rye (Secale cereale) had no distinguishable effect on population densities of P. brachyurus or T. christiei. Meloidogyne incognita was detected during the fourth year in both newly cleared and old agricultural land when okra was included in the cropping system. Detectable populations of M. incognita did not develop in any of the other cropping systems. Yields of tomato transplants were higher on the newly cleared land than on the old land. Highest yields were obtained when crotalaria was included in the cropping system. Lowest yields were obtained when milo, or fallow were included in the cropping system. Length of rotation had no distinguishable effect on yields of tomato transplants.

  15. The complete chloroplast genome sequence of Hibiscus syriacus.

    Kwon, Hae-Yun; Kim, Joon-Hyeok; Kim, Sea-Hyun; Park, Ji-Min; Lee, Hyoshin

    2016-09-01

    The complete chloroplast genome sequence of Hibiscus syriacus L. is presented in this study. The genome is composed of 161 019 bp in length, with a typical circular structure containing a pair of inverted repeats of 25 745 bp of length separated by a large single-copy region and a small single-copy region of 89 698 bp and 19 831 bp of length, respectively. The overall GC content is 36.8%. One hundred and fourteen genes were annotated, including 81 protein-coding genes, 4 ribosomal RNA genes and 29 transfer RNA genes.

  16. Isolation and identification of an allelopathic substance from Hibiscus sabdariffa.

    Suwitchayanon, Prapaipit; Pukclai, Piyatida; Ohno, Osamu; Suenaga, Kiyotake; Kato-Noguchi, Hisashi

    2015-05-01

    In this study, an allelopathic substance was isolated from an aqueous methanol extract of Hibiscus sabdariffa L. by column chromatography and reverse phase HPLC. The chemical structure of the substance was determined by 1H NMR spectroscopy and mass spectrometry as trimethyl allo-hydroxycitrate. Trimethyl allo-hydroxycitrate inhibited the growth of cress hypocotyls and roots at concentrations greater than 10 mM. The concentrations required for 50% growth inhibition of the hypocotyls and roots of cress were 20.3 and 14.4 mM, respectively. The inhibitory activity of trimethyl allo-hydroxycitrate suggests that the substance may act as an allelopathic substance of H. sabdariffa.

  17. Kombucha Rosella (Hibiscus sabdariffa Linn) dan Kemampuannya sebagai Antihiperkolesterolemia

    Suhartatik, Nanik; Karyantina, Merkuria; Purwanti, Indrias Tri

    2012-01-01

    We know that there is another tea extract, e.i from calyx of roselle flower (Hibiscus sabdariffa Linn). This kind of tea could be made became kombucha by ferment roselle extract using microbe in the fermentation of kombucha. This mi­ crobe grown in roselle extract as a medium with variety of roselle concentration (30; 40; 50 grams of dried roselle/L). During the fermentation process, roselle kombucha was analyzed for pH value, total acid, antioxidant activity at 0, 1, 3, 5, 7, 10 days of ferm...

  18. Hibiscus vitifolius (Linn.) root extracts shows potent protective action against anti-tubercular drug induced hepatotoxicity.

    Samuel, Anbu Jeba Sunilson John; Mohan, Syam; Chellappan, Dinesh Kumar; Kalusalingam, Anandarajagopal; Ariamuthu, Saraswathi

    2012-05-07

    The roots of Hibiscus vitifolius Linn. (Malvaceae) is used for the treatment of jaundice in the folklore system of medicine in India. This study is an attempt to evaluate the hepatoprotective activity of the roots of Hibiscus vitifolius against anti-tubercular drug induced hepatotoxicity. Hepatotoxicity was induced in albino rats of either sex by oral administration of a combination of three anti-tubercular drugs. Petroleum ether, chloroform, methanol and aqueous extracts of roots of Hibiscus vitifolius (400mg/kg/day) were evaluated for their possible hepatoprotective potential. All the extracts were found to be safe up to a dose of 2000mg/kg. Among the four extracts studied, oral administration of methanol extract of Hibiscus vitifolius at 400mg/kg showed significant difference in all the parameters when compared to control. There was a significant (PHibiscus vitifolius have potent hepatoprotective activity, thereby justifying its ethnopharmacological claim. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

  19. A survey of the microflora of Hibiscus sabdariffa (Roselle) and the ...

    hope&shola

    2006-02-02

    Feb 2, 2006 ... The dried calyx of Hibiscus sabdariffa (Roselle) is locally processed into a .... determined by perception with the sense organ upon opening a fresh .... Laboratory Zobo. Fresh. 2 wks old. Fresh. 2 wks old. Bacillus subtilis. +. +.

  20. Extraction of total polyphenols from hibiscus (Hibiscus sabdariffa L. and waxweed / ‘sete-sangrias’ (Cuphea carthagenensis and evaluation of their antioxidant potential

    Daniele Begmeier

    2014-02-01

    Full Text Available Current research investigates the extraction process of total polyphenols from hibiscus (Hibiscus sabdariffa L. and waxweed (Brazilian name: ‘sete-sangrias’ (Cuphea carthagenensis and evaluates the antioxidant potential of their extracts. The extraction stage comprised investigation on the following parameters: i solvents (acetone and ethanol pure and fractioned with water; ii variables (temperature, stirring, solvent ratio, time and pH. Total polyphenols were quantified by Folin-Ciocalteau reagent and antioxidant activity was determined by ABTS•+ (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid and DPPH (2,2-diphenyl-1-picrylhydrazyl radical scavenging assay. Results showed that, depending on experimental conditions, total phenolic contents for hibiscus and waxweed ranged between 460.86 mg GAE 100 g-1 and 5012.54 mg GAE 100 g-1 and between 462.86 mg GAE 100 g-1 and 4215.99 mg GAE 100 g-1, respectively. Waxweed had a higher antioxidant activity when compared to that of hibiscus by both ABTS•+ and DPPH. Data showed that hibiscus and waxweed have a significant amount of polyphenols which may be extracted in mild processing conditions and then employed as natural antioxidant sources in industrial processes.

  1. Analysis of MINT hibiscus rosa-sinensis mutants

    Shakinah Salleh; Shuhaimi Shamsudin; Putri Noor Faizah MM Tahir; Mohd Nazir Basiran

    2006-01-01

    Gamma irradiation is an alternative method to create new cultivars of Hibiscus rosa-sinensis in Malaysia. Increasing gamma ray doses resulted in a reduction of growing shoots on the irradiated stem cuttings. The LD50 for the stem cuttings was 36.2 Gy and at 75% growth was 17.15 Gy. Based on these results, 20 to 30 Gy were chosen for irradiation of the stem cuttings to induce mutation in Hibiscus rosa-sinensis. Irradiated stem cuttings were then planted into sand-beds for rooting. After one month, the rooted stems were transferred into polybags and allowed to grow under 70% shade provided by plastic netting and the variants were subsequently observed. Three new cultivars with variations in flower size and colours were obtained. The plants were propagated for five generations to achieve the stable mutants. Anthocyanins analysis is being carried out to measure the amount of anthocyanins in each cultivar. Pelargonidin chloride, Cyanidin chloride and Delphinidin chloride are used as standards for the analysis. (Author)

  2. Hibiscus sabdariffa L. - a phytochemical and pharmacological review.

    Da-Costa-Rocha, Inês; Bonnlaender, Bernd; Sievers, Hartwig; Pischel, Ivo; Heinrich, Michael

    2014-12-15

    Hibiscus sabdariffa L. (Hs, roselle; Malvaceae) has been used traditionally as a food, in herbal drinks, in hot and cold beverages, as a flavouring agent in the food industry and as a herbal medicine. In vitro and in vivo studies as well as some clinical trials provide some evidence mostly for phytochemically poorly characterised Hs extracts. Extracts showed antibacterial, anti-oxidant, nephro- and hepato-protective, renal/diuretic effect, effects on lipid metabolism (anti-cholesterol), anti-diabetic and anti-hypertensive effects among others. This might be linked to strong antioxidant activities, inhibition of α-glucosidase and α-amylase, inhibition of angiotensin-converting enzymes (ACE), and direct vaso-relaxant effect or calcium channel modulation. Phenolic acids (esp. protocatechuic acid), organic acid (hydroxycitric acid and hibiscus acid) and anthocyanins (delphinidin-3-sambubioside and cyanidin-3-sambubioside) are likely to contribute to the reported effects. More well designed controlled clinical trials are needed which use phytochemically characterised preparations. Hs has an excellent safety and tolerability record. Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.

  3. Deciduous shrubs for ozone bioindication: Hibiscus syriacus as an example

    Paoletti, Elena [Institut Plant Protection (IPP), National Council Research (CNR), Via Madonna del Piano 10, 50019 Sesto Fiorentino, Florence (Italy)], E-mail: e.paoletti@ipp.cnr.it; Ferrara, Anna Maria [Istituto per le Piante da Legno e l' Ambiente (IPLA), Corso Casale 476, 10132 Turin (Italy); Calatayud, Vicent; Cervero, Julia [Fundacion C.E.A.M., Charles R. Darwin 14, Parc Tecnologic, 46980 Paterna, Valencia (Spain); Giannetti, Fabio [Istituto per le Piante da Legno e l' Ambiente (IPLA), Corso Casale 476, 10132 Turin (Italy); Sanz, Maria Jose [Fundacion C.E.A.M., Charles R. Darwin 14, Parc Tecnologic, 46980 Paterna, Valencia (Spain); Manning, William J. [Department of Plant, Soil and Insect Sciences, University of Massachusetts, Amherst, MA 01003-9320 (United States)

    2009-03-15

    Ozone-like visible injury was detected on Hibiscus syriacus plants used as ornamental hedges. Weekly spray of the antiozonant ethylenediurea (EDU, 300 ppm) confirmed that the injury was induced by ambient ozone. EDU induced a 75% reduction in visible injury. Injury was more severe on the western than on the eastern exposure of the hedge. This factor of variability should be considered in ozone biomonitoring programmes. Seeds were collected and seedlings were artificially exposed to ozone in filtered vs. not-filtered (+30 ppb) Open-Top Chambers. The level of exposure inducing visible injury in the OTC seedlings was lower than that in the ambient-grown hedge. The occurrence of visible injury in the OTC confirmed that the ozone sensitivity was heritable and suggested that symptomatic plants of this deciduous shrub population can be successfully used as ozone bioindicators. EDU is recommended as a simple tool for diagnosing ambient ozone visible injury on field vegetation. - An Italian population of the deciduous shrub Hibiscus syriacus, a common ornamental species in temperate zones, is recommended as ozone bioindicator.

  4. Deciduous shrubs for ozone bioindication: Hibiscus syriacus as an example

    Paoletti, Elena; Ferrara, Anna Maria; Calatayud, Vicent; Cervero, Julia; Giannetti, Fabio; Sanz, Maria Jose; Manning, William J.

    2009-01-01

    Ozone-like visible injury was detected on Hibiscus syriacus plants used as ornamental hedges. Weekly spray of the antiozonant ethylenediurea (EDU, 300 ppm) confirmed that the injury was induced by ambient ozone. EDU induced a 75% reduction in visible injury. Injury was more severe on the western than on the eastern exposure of the hedge. This factor of variability should be considered in ozone biomonitoring programmes. Seeds were collected and seedlings were artificially exposed to ozone in filtered vs. not-filtered (+30 ppb) Open-Top Chambers. The level of exposure inducing visible injury in the OTC seedlings was lower than that in the ambient-grown hedge. The occurrence of visible injury in the OTC confirmed that the ozone sensitivity was heritable and suggested that symptomatic plants of this deciduous shrub population can be successfully used as ozone bioindicators. EDU is recommended as a simple tool for diagnosing ambient ozone visible injury on field vegetation. - An Italian population of the deciduous shrub Hibiscus syriacus, a common ornamental species in temperate zones, is recommended as ozone bioindicator

  5. Colorimetric Analysis of Hibiscus Beverages and their Potential Antioxidant Properties.

    Camelo-Méndez, G A; Vanegas-Espinoza, P E; Escudero-Gilete, M L; Heredia, F J; Paredes-López, O; Del Villar-Martínez, A A

    2018-05-25

    In food industry, roselle beverages and their subproducts could be functional ingredients since they are an excellent source of bioactive compounds with improved performance due to their important anthocyanins content. The aim of this study was to analyze anthocyanin content and antioxidant properties of aqueous infusions elaborated with color contrasting Hibiscus materials and design a mathematical model in order to predict color-composition relationship. Color measurements of beverages from roselle (Negra, Sudan and Rosa) were made by transmission spectrophotometry, anthocyanins quantification was determined by HPLC, and antioxidant potential was evaluated by in vitro methods (ABTS and FRAP assays). Beverages prepared with particle size minor of 250 μm presented until 4- and 2- times more anthocyanins content and antioxidant capacity respectively, in comparison to beverages prepared with powders with particle size major of 750 μm. Positive correlations among pigments composition and color parameters were found (p Hibiscus beverages with high anthocyanin content. The obtained models could be an important tool to be used in food industry for pigment characterization or functional compounds with potential health benefits.

  6. Hepatoprotective and Antioxidant Effect of Hibiscus Polyphenol Rich Extract (HPE) Against Carbon Tetrachloride (CCL4) - Induced Damage in Rats

    Adetutu, Adewale; Owoade, Abiodun O.

    2013-01-01

    Aims: Hibiscus sabdariffa is a medicinal plant that is consumed for its health benefits in Africa. The study was designed to investigate the hepatoprotective potentials of Hibiscus polyphenolic rich extract (HPE), (a group of phenolic compounds occurring in the dried calyx of Hibiscus sabdariffa) against CCl4-induced damaged in rats. Place and Duration of Study: Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomosho, Nigeria, between January 2011 and June 2012. Method...

  7. First case report on pathogenic fungus Fonsecaea sp. Negroni from skin of Pelophylax kl. esculentus L. in Serbia

    Stupar Miloš Č.

    2017-01-01

    Full Text Available Non-harmful adhesive tape method was applied directly on the skin surface of edible frog (Pelophylax kl. esculentus, captured in vernal ponds on the locality “Stevanove ravnice” within the Special Nature Reserve „Deliblatska peščara“, in order to detect fungal dwellers of frogs’ skin. Light microscopy analyses of Lactophenol Cotton Blue mounted adhesive tape samples taken from frog’s ventrum revealed the presence of melanized septate hyphae, branched conidiophores with chains of single-celled ovoid conidia, arising directly from the skin, which corresponds to morphological features of dematiaceous hyphomycete - Fonsecaea sp. Since members of genus Fonsecaea are frequently cited as causative agents of chromomycosis in amphibians, as well as human phaeohyphomycosis, world­wide, it is of great significance to study the presence of this fungal pathogen on amphibians in Serbia in order to make the basic reference data of the incidence of these pathogens in this region. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 173032

  8. Pharmacological characterization of the diuretic effect of Hibiscus sabdariffa Linn (Malvaceae) extract.

    Alarcón-Alonso, Javier; Zamilpa, Alejandro; Aguilar, Francisco Alarcón; Herrera-Ruiz, Maribel; Tortoriello, Jaime; Jimenez-Ferrer, Enrique

    2012-02-15

    Hibiscus sabdariffa L. (Malvaceae) populary known in Mexico as "Jamaica", "flor de Jamaica", has widely used in Mexican Traditional Medicine as antihypertensive and diuretic, although the latter activity has been reported the present work show evidence about the diuretic, natriuretic and potassium-sparing effects. To evaluate the diuretic activity of Hibiscus sabdariffa aqueous extract on in vivo and in situ models. The Hibiscus sabdariffa aqueous extract was administrated in increasing doses and evaluated the diuresis produced and disposal of electrolytes. Moreover, in isolated kidney was determined the renal filtration rate with plant extract, furosemide and amiloride. The yield of Hibiscus sabdariffa aqueous extraction was 28.3% and the chemical standardization from 1 g of extract was: 56.5 mg delphinidin-3-O-sambubioside, 20.8 mg/g cyanidin-3-O-sambubioside, 3.2 mg/g quercetin, 2.1 mg/g rutin and 2.7 mg/g chlorogenic acid. The diuretic and natriuretic effect of Hibiscus sabdariffa aqueous extract showed a dose-dependent behavior. The pharmacological constants of natriuretic effect was ED50=86 mg/kg and Emax=0.9 mEq/100 g/5 h. In the model of kidney in situ was observed that renal filtration increased 48% with the aqueous extract of Hibiscus sabdariffa and an additive effect when was perfuse with furosemide. The compound presents in Hibiscus sabdariffa as quercetin had effect on the vascular endothelium causing oxide nitric release, increasing renal vasorelaxation by increasing kidney filtration. Therefore, the diuretic effect of Hibiscus sabdariffa may be mediated by nitric oxide release. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  9. Potensi Diversifikasi Rosela Herbal (Hibiscus Sabdariffa L.) untuk Pangan dan Kesehatan

    Nurnasari, Elda; Khuluq, Ahmad Dhiaul

    2017-01-01

    Tanaman penghasil serat alami dari genus Hibiscus yang cukup populer di Indonesia adalah rosela (Hibiscus sabdariffa L.). Nilai kemanfaatan rosela sangat luas baik untuk pangan dan kesehatan, sehingga potensi diversifikasi rosela cukup besar. Tujuan penulisan review ini adalah untuk memberikan informasi kandungan fitokimia potensial tanaman rosela herbal yang memiliki efek farmakologis dan fisiologis serta beberapa diversifikasi produk makanan, minuman dan produk kesehatan. Bagian tanaman ro...

  10. Hibiscus syriacus Extract from an Established Cell Culture Stimulates Skin Wound Healing

    di Martino, O.; Tito, A.; De Lucia, A.; Cimmino, A.; Cicotti, F.; Apone, F.; Colucci, G.; Calabrò, V.

    2017-01-01

    Higher plants are the source of a wide array of bioactive compounds that support skin integrity and health. Hibiscus syriacus, family Malvaceae, is a plant of Chinese origin known for its antipyretic, anthelmintic, and antifungal properties. The aim of this study was to assess the healing and hydration properties of H. syriacus ethanolic extract (HSEE). We established a cell culture from Hibiscus syriacus leaves and obtained an ethanol soluble extract from cultured cells. The properties of th...

  11. Pollen and Anther Cultures as Potential Means in Production of Haploid Kenaf (Hibiscus Cannabinus L.)

    Susanto, Dwi; Ibrahim, Ahmed Mahmood; Mat Hussin, Zeti Ermiena Surya

    2013-01-01

    Kenaf (Hibiscus cannabinus L.) is one of the important species of Hibiscus for human needs. It is annual upright plant that produces high biomass, has good adaptability to its environment and inexpensive to be grown. Kenaf has been cultivated in many countries since long time ago mainly for fibers. In Malaysia, however, this plant is considered new and cultivated especially in the eastern parts of Peninsular to replace tobacco plantations that have been no longer supported by the government. ...

  12. Elements of kitchen toxicology to exploit the value of traditional (African recipes: The case of Egusi Okra meal in the diet of HIV+/AIDS subjects

    Chiara Frazzoli

    Full Text Available The Egusi Okra soup is a traditional African meal that is considered of high nutritional value and protective against weight loss. We introduce the concept of “kitchen toxicology” to analyse the recipe of the Egusi Okra soup and highlight possible mitigation measures for toxic and/or antinutritional effects in the wide spectrum of health and nutritional needs of HIV+/AIDS subjects. In particular, we focus on toxicants (environmental contaminants, process contaminants, substances leaching from food contact materials dysregulating the immune status, as well as on interactions between nutrients, contaminants, and/or antinutrients which may lead to secondary/conditioned nutritional deficiencies or imbalances; in their turn, these can modulate the ability to cope with toxicants, and increase nutritional requirements. Recommendations are given for practices preserving the Egusi Okra soup from such risk factors, identifying points of particular attention during meal preparation, from purchase of raw ingredients through to food handling, cooking, storage, and consumption. The Egusi Okra soup is discussed in the context of a diet that is asked to mitigate complications (weight loss, opportunistic infections and support antiretroviral therapy in African countries with high HIV/AIDS prevalence. The paper discusses how nutritional interventions benefit of the integration of kitchen toxicology practices in everyday life. Toxicological risk assessment is crucial to understand the history and status of the person exposed to or affected by infectious diseases. Keywords: Traditional diet, Malnutrition, Food safety, Weight loss, Immune system, Dysmetabolic diseases, Clinical toxicology, Nutrition security

  13. Studies on the antifertility potentiality of Hibiscus rosa sinensis. Parts of medicinal value; selection of species and seasonal variations.

    Kholkute, S D; Mudgal, V; Udupa, K N

    1977-02-01

    The postcoital antifertility properties of benzene hot extracts of Hibiscus rosa sinensis flowers, leaves, and stembarks, collected during the winter, spring, rainy, and summer seasons, were investigated in female rats. Only extracts from the flowers of the plant were 100% effective in preventing pregnancy. Those flowers collected during the winter showed the greatest potency, followed by those collected in the spring, rainy season, and summer, in decreasing order. Benzene extracts of flowers collected from Hibiscus mutabilis, Hibiscus schizopetalus, and Malvasicus grandiflorus, plants resembling Hibiscus rosa sinensis in petaloid structure, did not markedly affect pregnancy.

  14. Effect of extract of Hibiscus on the ultrastructure of the testis in adult mice.

    Mahmoud, Yomna Ibrahim

    2012-07-01

    Hibiscus sabdariffa extract is a popular beverage in many tropical and sub-tropical countries. Although, Hibiscus tea is known for its medicinal effects for thousands of years, scientific evidence of its systemic safety is very limited. The current study aimed to assess the potential adverse effects of H. sabdariffa extract on sperm morphology and testicular ultrastructure of albino mice. Thirty adult male albino mice were divided into three equal groups and were given: (a) distilled water, (b) cold Hibiscus aqueous extract, and (c) boiled Hibiscus aqueous extract. Hibiscus extract was administered orally daily for 4 weeks in a dose of 200 mg/kg body weight/mouse. Twenty-four hours after the last treatment, mice were decapitated and the testes and epididymides were excised and processed for transmission electron microscopy to assess ultrastructural and sperm abnormalities. The results clearly demonstrate that aqueous extracts from dried calyx of H. sabdariffa, either cold or boiled, alter normal sperm morphology and testicular ultrastructure and adversely influence the male reproductive fertility in albino mice. The current data suggest that Hibiscus extract should be consumed with caution, and reasonable estimates of the human risk associated with its consumption should be provided. Copyright © 2011 Elsevier GmbH. All rights reserved.

  15. Decrease of Plasma Glucose by Hibiscus taiwanensis in Type-1-Like Diabetic Rats

    Wang, Lin-Yu; Chung, Hsien-Hui

    2013-01-01

    Hibiscus taiwanensis (Malvaceae) is widely used as an alternative herb to treat disorders in Taiwan. In the present study, it is used to screen the effect on diabetic hyperglycemia in streptozotocin-induced diabetic rats (STZ-diabetic rats). The extract of Hibiscus taiwanensis showed a significant plasma glucose-lowering action in STZ-diabetic rats. Stems of Hibiscus taiwanensis are more effective than other parts to decrease the plasma glucose in a dose-dependent manner. Oral administration of Hibiscus taiwanensis three times daily for 3 days into STZ-diabetic rats increased the sensitivity to exogenous insulin showing an increase in insulin sensitivity. Moreover, similar repeated administration of Hibiscus taiwanensis for 3 days in STZ-diabetic rats produced a marked reduction of phosphoenolpyruvate carboxykinase (PEPCK) expression in liver and an increased expression of glucose transporter subtype 4 (GLUT 4) in skeletal muscle. Taken together, our results suggest that Hibiscus taiwanensis has the ability to lower plasma glucose through an increase in glucose utilization via elevation of skeletal GLUT 4 and decrease of hepatic PEPCK in STZ-diabetic rats. PMID:23690841

  16. The effectiveness of Hibiscus sabdariffa in the treatment of hypertension: a systematic review.

    Wahabi, H A; Alansary, L A; Al-Sabban, A H; Glasziuo, P

    2010-02-01

    Hypertension is a common global health problem with significant mortality and morbidity. Hibiscus sabdariffa is a plant known in many countries and is consumed as hot and cold drinks In addition to its use in folk medicine; it has been suggested as treatment for many conditions including hypertension. The objectives of this review were to examine the evidence of effectiveness and safety of hibiscus in the treatment of hypertension. We searched several medical databases (MEDLINE, EMBASE, CINAHL, Cochrane Central Register of Controlled Trials, and the specialized register of the Cochrane Hypertension Group and the general engine Google) to January 2009. We included randomized controlled trials that had examined Hibiscus's effectiveness and safety in the treatment of primary hypertension in adults. Two authors independently selected the trials for the review, extracted the data, and critically appraised the included studies. Four trials, with a total of 390 patients, met our inclusion criteria. Two studies compared Hibiscus sabdariffa to black tea; one study compared it to captopril and one to lisinopril. The studies found that Hibiscus had greater blood pressure reduction than tea but less than the ACE-inhibitors. However, all studies, except one, were short term and of poor quality with a Jadad scoring of Hibiscus sabdariffa for the treatment of primary hypertension in adults. Copyright 2009 Elsevier GmbH. All rights reserved.

  17. A review on phytochemistry and therapeutic uses of Hibiscus sabdariffa L.

    Riaz, Ghazala; Chopra, Rajni

    2018-06-01

    Hibiscus sabdariffa L. (roselle) belonging to the Malvaceae family is widely grown in many countries. This plant is often used in the traditional medicine being rich in phytochemicals like polyphenols especially anthocyanins, polysaccharides and organic acids thus having enormous prospective in modern therapeutic uses. The study aimed to review and document all the available evidence and information about the calyces of Hibiscus sabdariffa (roselle) with the special focus on their nutritional composition, bioactive constituents and therapeutic uses. The electronic database was searched up to 2017, using keywords Hibiscus sabdariffa, chemical constituents of roselle, therapeutic uses of roselle. Journals, books and conference proceedings were also searched. The review provides valuable information about the nutraceutical component of Hibiscus sabdariffa L. and their utilization for curing various degenerative diseases like hypertension, hyperlipidemia, cancer and other inflammatory diseases of liver and kidney. Their toxicological effects have also been discussed from a safety point of view. Most studies supported and provided the scientific basis for the statement that Hibiscus sabdariffa and their active constituents play an important role in the prevention of chronic and degenerative diseases that are associated with oxidative stress. Our study suggests, that good research is needed, to establish a potential strategy that can balance the pharmacological and toxic effects of roselle and standardized fingerprint of Hibiscus sabdariffa is required internationally for quality control. Copyright © 2018 Elsevier Masson SAS. All rights reserved.

  18. Spatial and temporal transcriptome changes occurring during flower opening and senescence of the ephemeral hibiscus flower, Hibiscus rosa-sinensis.

    Trivellini, Alice; Cocetta, Giacomo; Hunter, Donald A; Vernieri, Paolo; Ferrante, Antonio

    2016-10-01

    Flowers are complex systems whose vegetative and sexual structures initiate and die in a synchronous manner. The rapidity of this process varies widely in flowers, with some lasting for months while others such as Hibiscus rosa-sinensis survive for only a day. The genetic regulation underlying these differences is unclear. To identify key genes and pathways that coordinate floral organ senescence of ephemeral flowers, we identified transcripts in H. rosa-sinensis floral organs by 454 sequencing. During development, 2053 transcripts increased and 2135 decreased significantly in abundance. The senescence of the flower was associated with increased abundance of many hydrolytic genes, including aspartic and cysteine proteases, vacuolar processing enzymes, and nucleases. Pathway analysis suggested that transcripts altering significantly in abundance were enriched in functions related to cell wall-, aquaporin-, light/circadian clock-, autophagy-, and calcium-related genes. Finding enrichment in light/circadian clock-related genes fits well with the observation that hibiscus floral development is highly synchronized with light and the hypothesis that ageing/senescence of the flower is orchestrated by a molecular clock. Further study of these genes will provide novel insight into how the molecular clock is able to regulate the timing of programmed cell death in tissues. © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  19. Metal-Organic Frameworks Derived Okra-like SnO2 Encapsulated in Nitrogen-Doped Graphene for Lithium Ion Battery.

    Zhou, Xiangyang; Chen, Sanmei; Yang, Juan; Bai, Tao; Ren, Yongpeng; Tian, Hangyu

    2017-04-26

    A facile process is developed to prepare SnO 2 -based composites through using metal-organic frameworks (MOFs) as precursors. The nitrogen-doped graphene wrapped okra-like SnO 2 composites (SnO 2 @N-RGO) are successfully synthesized for the first time by using Sn-based metal-organic frameworks (Sn-MOF) as precursors. When utilized as an anode material for lithium-ion batteries, the SnO 2 @N-RGO composites possess a remarkably superior reversible capacity of 1041 mA h g -1 at a constant current of 200 mA g -1 after 180 charge-discharge processes and excellent rate capability. The excellent performance can be primarily ascribed to the unique structure of 1D okra-like SnO 2 in SnO 2 @N-RGO which are actually composed of a great number of SnO 2 primary crystallites and numerous well-defined internal voids, can effectively alleviate the huge volume change of SnO 2 , and facilitate the transport and storage of lithium ions. Besides, the structural stability acquires further improvement when the okra-like SnO 2 are wrapped by N-doped graphene. Similarly, this synthetic strategy can be employed to synthesize other high-capacity metal-oxide-based composites starting from various metal-organic frameworks, exhibiting promising application in novel electrode material field of lithium-ion batteries.

  20. Nigerian Journal of Chemical Research 1 Vol. 15, 2010 ...

    Hp

    University of Benin, GeoEnvironmental & Climate Change Adaptation ... transferability of the metal to okra (Abelmoschus esculentus) plant, and the long term ... to 85% in poultry manure amended soil for As. Post-harvest acid treatment of the ...

  1. Aqueous Extracts of Hibiscus sabdariffa Calyces to Control Aichi Virus.

    D'Souza, Doris H; Dice, Lezlee; Davidson, P Michael

    2016-06-01

    Aqueous Hibiscus sabdariffa extracts possess antimicrobial properties with limited information available on their antiviral effects. Aichi virus (AiV) is an emerging foodborne pathogen that causes gastroenteritis. Vaccines are currently unavailable to prevent their disease transmission. The objective of this study was to determine the antiviral effects of aqueous H. sabdariffa extracts against AiV. AiV at ~5 log PFU/ml was incubated with undiluted (200 mg/ml), 1:1 (100 mg/ml) or 1:5 (40 mg/ml) diluted aqueous hibiscus extract (pH 3.6), phosphate-buffered saline (pH 7.2 as control), or malic acid (pH 3.0, acid control) at 37 °C over 24 h. Treatments were stopped by serially diluting in cell-culture media containing fetal bovine serum and titers were determined using plaque assays on confluent Vero cells. Each treatment was replicated thrice and assayed in duplicate. AiV did not show any significant reduction with 1:1 (100 mg/ml) or 1:5 (40 mg/ml) diluted aqueous hibiscus extracts or malic acid after 0.5, 1, or 2 h at 37 °C. However, AiV titers were reduced to non-detectable levels after 24 h with all the three tested concentrations, while malic acid showed only 0.93 log PFU/ml reduction after 24 h. AiV was reduced by 0.5 and 0.9 log PFU/ml with undiluted extracts (200 mg/ml) after 2 and 6 h, respectively. AiV treated with 1:1 (100 mg/ml) and 1:5 (40 mg/ml) diluted extracts showed a minimal ~0.3 log PFU/ml reduction after 6 h. These extracts show promise to reduce AiV titers mainly through alteration of virus structure, though higher concentrations may have improved effects.

  2. Protocols for Callus and Somatic Embryo Initiation for Hibiscus sabdariffa L. (Malvaceae): Influence of Explant Type, Sugar, and Plant Growth Regulators

    A significant work on callus induction and somatic embryogenesis was realized for Hibiscus sabdariffa. Two genotypes (Hibiscus sabdariffa and Hibiscus sabdariffa var. altissima) two sugars (sucrose and glucose) and three concentrations (1 %, 2%, 3%) of each sugar, 3 explant types (root, hypocotyl, c...

  3. Review Hepatoprotektor Rosela (Hibiscus sabdariffa: Aktivitas, Mekanisme Aksi dan Toksisitas

    Steffi Liem

    2017-12-01

    Full Text Available Liver cirrhosis has become the international health problem that causes significant mortality. Handling using herbs from various countries has been done, one of them by consuming roselle (Hibiscus sabdariffa calyx extract. H. sabdariffa contains anthocyanins, flavonoids, tannins, and ascorbic acid that function as hepatoprotector. H. sabdariffacalyx extract may inhibit the increasing of various clinical parameters, such as ALT, AST, ALP, and LDH in various inducer types, ie acetaminophen, CCl4, CdCl2, DNPH, and TAA. The mechanism of action of H. sabdariffacalyx extract are as an antioxidant, inhibition of cytochrome enzyme, induction of phase II metabolism enzymes, increase cell viability percentage, increase CAT, GSH and decrease protein expression of pJNK, tBid and Bax. However, the consumption of H. sabdariffacalyx extract may also cause acute, subchronic, and chronic toxicity depending on the given dose.

  4. Vitiquinolone--a quinolone alkaloid from Hibiscus vitifolius Linn.

    Ramasamy, D; Saraswathy, A

    2014-02-15

    Phytochemical investigations of the powdered root of Hibiscus vitifolius Linn. (Malvaceae) was extracted successively with n-hexane and chloroform. Analysis of the n-hexane extract by GC-MS led to the identification of twenty-six components by comparison of their mass spectra with GC-MS library data. A novel quinolone alkaloid, vitiquinolone (5) together with eight known compounds viz. β-Amyrin acetate (1), n-octacosanol (2), β-Amyrin (3), stigmasterol (4), xanthyletin (6), alloxanthoxyletin (7), xanthoxyletin (8) and betulinic acid (9) were isolated from chloroform extract by column chromatography over silica gel. The structure of vitiquinolone was established on the basis of spectroscopic methods including UV, IR, 1D, 2D NMR and ESI-MS. The known compounds were identified on the basis of their physical and spectroscopic data as reported in the literature. Copyright © 2013 Elsevier Ltd. All rights reserved.

  5. Natural cross-pollination in roselle, Hibiscus sabdariffa L. (Malvaceae

    Vaidya K.R.

    2000-01-01

    Full Text Available Two local varieties of roselle (Hibiscus sabdariffa L., Jamaican Green and Jamaican Red, were grown to determine the amount of natural cross-pollination. Two planting arrangements (alternating rows; alternating individuals in a row and two planting dates, a month apart, were used for the outcrossing experiments. Stem pigmentation, red (R- vs. green (rr, was used as a genetic marker in the estimation of outcrossing. Homozygous dominant and recessive genotypes of Jamaican Red and Jamaican Green, respectively, were grown in both of the planting arrangements and dates. Seeds from open-pollinated capsules of randomly selected Jamaican Green plants were planted to score the frequency of cross-pollination. Estimates of natural cross-pollination ranged from 0.20 ± 0.09% to 0.68 ± 0.34%. Roselle outcrosses at a low rate in Jamaica.

  6. Hibiscus sabdariffa extract lowers blood pressure and improves endothelial function.

    Joven, Jorge; March, Isabel; Espinel, Eugenia; Fernández-Arroyo, Salvador; Rodríguez-Gallego, Esther; Aragonès, Gerard; Beltrán-Debón, Raúl; Alonso-Villaverde, Carlos; Rios, Lidia; Martin-Paredero, Vicente; Menendez, Javier A; Micol, Vicente; Segura-Carretero, Antonio; Camps, Jordi

    2014-06-01

    Polyphenols from Hibiscus sabdariffa calices were administered to patients with metabolic syndrome (125 mg/kg/day for 4 wk, n = 31) and spontaneously hypertensive rats (125 or 60 mg/kg in a single dose or daily for 1 wk, n = 8 for each experimental group). The H. sabdariffa extract improved metabolism, displayed potent anti-inflammatory and antioxidant activities, and significantly reduced blood pressure in both humans and rats. Diuresis and inhibition of the angiotensin I-converting enzyme were found to be less important mechanisms than those related to the antioxidant, anti-inflammatory, and endothelium-dependent effects to explain the beneficial actions. Notably, polyphenols induced a favorable endothelial response that should be considered in the management of metabolic cardiovascular risks. © 2014 The Authors. Molecular Nutrition & Food Research published by Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  7. Factors influencing extract of Hibiscus sabdariffa staining of rat testes.

    Bassey, R B; Bakare, A A; Peter, A I; Oremosu, A A; Osinubi, A A

    2012-08-01

    Some plant extracts can be used in biology and medicine to reveal or identify cellular components and tissues. We investigated the effects of time and concentration on staining of histological sections of rat testes by an acidified extract of Hibiscus sabdariffa. An ethanolic extract of H. sabdariffa was diluted using 1% acetic acid in 70% ethanol to stain histological sections of testes at concentrations of 0.2, 0.1 and 0.05 g/ml for 5, 10, 15, 30, 45 and 60 min. The sections of testes were stained deep red. The staining efficiency of H. sabdariffa was greater at a high concentration and required less time to achieve optimal staining. H. sabdariffa is a strongly basic dye that can be used for various diagnostic purposes. Staining time and concentration must be considered to achieve optimal results.

  8. Assessment of oil content and fatty acid composition variability in two economically important Hibiscus species.

    Wang, Ming Li; Morris, Brad; Tonnis, Brandon; Davis, Jerry; Pederson, Gary A

    2012-07-04

    The Hibiscus genus encompasses more than 300 species, but kenaf (Hibiscus cannabinus L.) and roselle (Hibiscus sabdariffa L.) are the two most economically important species within the genus. Seeds from these two Hibiscus species contain a relatively high amount of oil with two unusual fatty acids: dihydrosterculic and vernolic acids. The fatty acid composition in the oil can directly affect oil quality and its utilization. However, the variability in oil content and fatty acid composition for these two species is unclear. For these two species, 329 available accessions were acquired from the USDA germplasm collection. Their oil content and fatty acid composition were determined by nuclear magnetic resonance (NMR) and gas chromatography (GC), respectively. Using NMR and GC analyses, we found that Hibiscus seeds on average contained 18% oil and seed oil was composed of six major fatty acids (each >1%) and seven minor fatty acids (each Hibiscus cannabinus seeds contained significantly higher amounts of oil (18.14%), palmitic (20.75%), oleic (28.91%), vernolic acids (VA, 4.16%), and significantly lower amounts of stearic (3.96%), linoleic (39.49%), and dihydrosterculic acids (DHSA, 1.08%) than H. sabdariffa seeds (17.35%, 18.52%, 25.16%, 3.52%, 4.31%, 44.72%, and 1.57%, respectively). For edible oils, a higher oleic/linoleic (O/L) ratio and lower level of DHSA are preferred, and for industrial oils a high level of VA is preferred. Our results indicate that seeds from H. cannabinus may be of higher quality than H. sabdariffa seeds for these reasons. Significant variability in oil content and major fatty acids was also detected within both species. The variability in oil content and fatty acid composition revealed from this study will be useful for exploring seed utilization and developing new cultivars in these Hibiscus species.

  9. Tolerance of chufa (Cyperus esculentus) as a vegetation unit's representative of bioregenerative life support systems to elevated temperatures

    Shklavtsova, Ekaterina; Ushakova, Sofya; Shikhov, Valentin; Kudenko, Yurii

    Plants inclusion in the photosynthesizing unit of bioregenerative life support systems (BLSS) expects knowledge of both production characteristics of plants cultivated under optimal condi-tions and their tolerance to stress-factors' effect caused by contingency origination in a system. The work was aimed at investigation of chufa (Cyperus esculentus) tolerance to the effect of super optimal air temperature of 44 subject to PAR intensity and exposure duration. Chufa was grown in light culture conditions by hydroponics method on expanded clay aggregate. The Knop solution was used as nutrition medium. Up to 30 days the plants were cultivated at the intensity of 690 micromole*m-2*s*-1 and air temperature of 25. Heat shock was employed at the age of 30 days under the air temperature of 44 during 7, 20 and 44 hours at two different PAR intensities of 690 and 1150 micromole*m-2*s*-1. Chufa heat tolerance was estimated by intensity of external 2 gas exchange and by state of leaves' photosynthetic apparatus (PSA). Effect of disturbing temperature during 44 hours at PAR intensity of 690 micromole*m-2*s*-1 resulted in frozen-in damage of PSA-leaves' die-off. Chufa plants exposed to heat stress at PAR intensity of 690 micromole*m-2*s*-1 during both 7 and 20-hours demonstrated respiration dominance over photosynthesis; and 2 emission was observed by light. Functional activity of photosynthetic apparatus estimated with respect to parameters of pulse-amplitude-modulated chlorophyll fluorescence of photosystem 2 (PS 2) decreased on 40

  10. Notice to nurserymen of the nameing and release for propagation of lufin white an american hardy hibiscus cultivar

    Hibiscus laevis ‘LUFKIN WHITE’, a new hardy native ornamental hibiscus cultivar is recommended for trial by nurserymen and horticulturists as a summer flowering perennial landscape plant nationwide but is particularly adapted to conditions in the South. ‘LUFKIN WHITE’ was selected for its exception...

  11. JST Thesaurus Headwords and Synonyms: Hibiscus cannabinus [MeCab user dictionary for science technology term[Archive

    Full Text Available MeCab user dictionary for science technology term Hibiscus cannabinus 名詞 一般 * * * *... ケナフ ケナフ ケナフ Thesaurus2015 200906017528823668 C LS06 UNKNOWN_2 Hibiscus cannabinus

  12. JST Thesaurus Headwords and Synonyms: Hibiscus [MeCab user dictionary for science technology term[Archive

    Full Text Available MeCab user dictionary for science technology term Hibiscus 名詞 一般 * * * * フヨウ属 フヨウゾク フヨーゾク Thesaurus2015 200906098519206796 C LS06 UNKNOWN_1 Hibiscus

  13. Descriptores para la caracterización y registro de variedades cubanas de Flor de Jamaica (Hibiscus sabdariffa L.)

    González Cepero, María C

    2014-01-01

    Se describen las principales características de la Flor de Jamaica (Hibiscus sabdariffa L.) así como el momento y la forma de evaluación The main characteristics of the Flor de Jamaica (Hibiscus sabdariffa L.) are described as well as the evaluation period and evaluation way

  14. Comparison of metabolic profiles and bioactivities of the leaves of three edible Congolese Hibiscus species.

    Kapepula, Paulin Mutwale; Kabamba Ngombe, Nadege; Tshisekedi Tshibangu, Pascal; Tsumbu, César; Franck, Thierry; Mouithys-Mickalad, Ange; Mumba, Dieudonné; Tshala-Katumbay, Désiré; Serteyn, Didier; Tits, Monique; Angenot, Luc; Kalenda, Pascal Dibungi T; Frédérich, Michel

    2017-12-01

    Methanolic and dichloromethane extracts from the leaves of Congolese Hibiscus species were characterised by chromatographic and spectroscopic techniques and their in vitro biochemical activities against ROS production were evaluated in cellular models and on an enzyme, myeloperoxidase (MPO), involved in inflammation. Hibiscus acetosella has a chemical fingerprint different from Hibiscus cannabinus and Hibiscus sabdariffa both having similar fingerprints. Major compounds were polyphenols, represented mainly by caffeoyl-hydroxycitric acid for H. acetosella and neochlorogenic acid for the two other species. All extracts displayed high cellular antioxidant activity with IC 50 values ranging from 0.5 to 3 μg mL -1 using lucigenin on neutrophils. Dichloromethane extracts showed more efficient effects on extracellular ROS production and MPO activity. Antioxidant and anti-inflammatory activities of caffeoyl-hydroxycitric acid were significantly higher than those of neochlorogenic acid. The bioactivities of Hibiscus species were positively correlated with their phytochemical content and could justify their use as local nutraceutical resources and medicines.

  15. Xyloglucan, hibiscus and propolis for the prevention of urinary tract infections: results of in vitro studies.

    Fraile, Benito; Alcover, Javier; Royuela, Mar; Rodríguez, David; Chaves, Concepción; Palacios, Ricardo; Piqué, Núria

    2017-06-01

    To assess the properties of a medical device containing xyloglucan, propolis and hibiscus to create a bioprotective barrier to avoid the contact of uropathogenic Escherichia coli strains on cell walls in models of intestinal (CacoGoblet) and uroepithelial (RWPE-1) cells (derived from normal human prostate epithelium). Two uropathogenic E. coli strains (expressing type 1 fimbriae and P fimbriae) were used to assess, by electronic microscopy and ELISA, the barrier properties of the medical device. The antimicrobial activity was assessed in broth dilution assays. The three components (xyloglucan, propolis and hibiscus) did not alter E. coli cell integrity in intestinal and uroepithelial cell models and were devoid of antibacterial activity. The three components avoided bacterial contact in both cell monolayers. The nonpharmacological barrier properties of xyloglucan, propolis and hibiscus confirm the role of the medical device for the management of urinary tract infections.

  16. [Pharmacological study on hemostasis, analgesic and anti inflammation effects of the alcohol extract of Hibiscus tiliaceus].

    Qiu, Fen; Tian, Hui; Zhang, Zhi; Yuan, Xian-Ling; Tan, Yuan-Feng; Ning, Xiao-Qing

    2013-10-01

    To study the effects of hemostasis, analgesic and anti inflammation of the alcohol extract of Hibiscus tiliaceus and offer pharmacological and experimental basis for its safe and effective use in clinic. The effects of hemostasist were observed with tail breaking method, capillary tube method and slide method; Hot board and body distortion induced by acetic acid methods were applied in mice analgesia experiment, the mice model of acute auricle swelling induced by dmi ethylbenzene and capillary permeability induced by acetic acid were applied to observe the anti inflammatory effects. The alcohol extract of Hibiscus tiliaceus could significantly reduce the bleeding time and the clotting time, delay the plant reaction time and reduce the writhing times of the mice, and it also had effect on inhibiting swelling of mice ear and the permeability of the capillary. These results suggest that the alcohol extract of Hibiscus tiliaceus has the effects of hemostasis, analgesic and anti inflammation.

  17. Breeding of Hibiscus rosa-sinensis for garden use in Denmark

    Kuligowska, Katarzyna; Simonsen, Maria Boye; Lütken, Henrik Vlk

    2013-01-01

    -sinensis for increased chilling tolerance by conventional breeding is unlikely because there is a lack of genetic information in relation to this trait within this species. In the Hibiscus genus, cold hardiness is available, but only in a few species. Attempts to create cold tolerant Hibiscus plants resembling H. rosa......Hibiscus rosa-sinensis is an ornamental shrub in tropical and subtropical regions. Limitation of its use as an outdoor ornamental plant in a temperate climate is due to chilling sensitivity. Cold hardiness is a highly complex trait, involving multiple genes. Improvement of H. rosa......-sinensis suitable for Nordic climate have not been successful so far. In ornamental breeding wide hybridization is a major source of genetic variation, and can also be a promising approach to introgress cold hardiness in H. rosa-sinensis. The objective of the present study is to evaluate the possibilities...

  18. Hibiscus sabdariffa L. tea (tisane) lowers blood pressure in prehypertensive and mildly hypertensive adults.

    McKay, Diane L; Chen, C-Y Oliver; Saltzman, Edward; Blumberg, Jeffrey B

    2010-02-01

    In vitro studies show Hibiscus sabdariffa L., an ingredient found in many herbal tea blends and other beverages, has antioxidant properties, and, in animal models, extracts of its calyces have demonstrated hypocholesterolemic and antihypertensive properties. Our objective in this study was to examine the antihypertensive effects of H. sabdariffa tisane (hibiscus tea) consumption in humans. A randomized, double-blind, placebo-controlled clinical trial was conducted in 65 pre- and mildly hypertensive adults, age 30-70 y, not taking blood pressure (BP)-lowering medications, with either 3 240-mL servings/d of brewed hibiscus tea or placebo beverage for 6 wk. A standardized method was used to measure BP at baseline and weekly intervals. At 6 wk, hibiscus tea lowered systolic BP (SBP) compared with placebo (-7.2 +/- 11.4 vs. -1.3 +/- 10.0 mm Hg; P = 0.030). Diastolic BP was also lower, although this change did not differ from placebo (-3.1 +/- 7.0 vs. -0.5 +/- 7.5 mm Hg; P = 0.160). The change in mean arterial pressure was of borderline significance compared with placebo (-4.5 +/- 7.7 vs. -0.8 +/- 7.4 mm Hg; P = 0.054). Participants with higher SBP at baseline showed a greater response to hibiscus treatment (r = -0.421 for SBP change; P = 0.010). No effects were observed with regard to age, gender, or dietary supplement use. These results suggest daily consumption of hibiscus tea, in an amount readily incorporated into the diet, lowers BP in pre- and mildly hypertensive adults and may prove an effective component of the dietary changes recommended for people with these conditions.

  19. Períodos de interferência de plantas daninhas na cultura do quiabo Periods of weed interference in okra crop

    L.P.S Bachega

    2013-03-01

    Full Text Available Poucas pesquisas têm sido realizadas sobre interferência de plantas daninhas na cultura do quiabo. Objetivou-se com este trabalho estimar os períodos de interferência da comunidade infestante no quiabeiro. Um experimento de campo foi conduzido sob dois grupos de tratamentos, mantendo períodos crescentes de 0 (testemunha, 7, 21, 28, 35, 42, 49, 63, 77, 91 e 105 dias após a emergência da cultura (DAE, com e sem controle das plantas daninhas. As plantas daninhas com maior importância relativa foram Portulaca oleracea, Nicandra physaloides e Eleusine indica. A convivência do quiabeiro com as plantas daninhas por todo o ciclo de cultivo reduziu a produtividade da cultura em 95%. O período anterior à interferência foi de 57 DAE, enquanto o período total de prevenção à interferência foi de 14 DAE. Não houve período crítico de prevenção à interferência, sendo um único controle das plantas daninhas entre 14 e 57 DAE suficiente para prevenir a interferência na cultura do quiabo.Few research works have been carried out on weed interference in okra crop. The aim of this study was to estimate the periods of weed interference in okra crop. Thus, a field trial was carried out under two groups of treatments, using increased periods of 0 (check, 7, 21, 28, 35, 42, 49, 63, 77, 91, and 105 days after crop emergence (DAE with weed control and without weed control. Weeds with highest relative importance were Portulaca oleracea, Nicandra physaloides, and Eleusine indica. Coexistence of okra crop and weeds throughout the season reduced crop yield by 95%. The period before interference was 57 DAE, while total period of interference prevention was 14 DAE. There was no critical period of interference prevention, with a single weed control between 14 and 57 DAE being sufficient for interference prevention in okra crop.

  20. Gamete types, sex determination and stable equilibria of all-hybrid populations of diploid and triploid edible frogs (Pelophylax esculentus

    Christiansen Ditte G

    2009-06-01

    Full Text Available Abstract Background Triploid individuals often play a key role in speciation by hybridization. An understanding of the gamete types (ploidy and genomic content and stability of hybrid populations with triploid individuals is therefore of importance for exploring the role of hybridization in evolution. The all-hybrid populations of the edible frog, Pelophylax esculentus, are unique in their composition and genetic dynamics: Diploid (genotype LR and triploid (LLR and LRR hybrids depend on each other's different gamete contributions for successful reproduction and maintenance of the populations, as the parental genotypes P. lessonae (LL and P. ridibundus (RR are absent among adults. This study provides data and interpretations on gamete types and sex determination that are essential for understanding the function, evolutionary potential and threats of this intriguing system. Results Dissection of metamorphs from a crossing experiment confirmed that sex determination is an XX-XY system with the Y confined to the L genome. From microsatellite analysis of parents and offspring from the crossings, gamete frequencies could be deduced: Triploids of both sexes mostly made haploid gametes with the genome they had in double dose, however LLR females also made approximately 10% LL gametes by automixis. LR frogs showed much variation in their gamete production. In LRR-rich populations, their LR sperm production was sufficiently high (22% to explain the observed proportion of LRR males, the formation of which has not previously been understood. A model was constructed to calculate equilibrium genotype proportions for different population types on the basis of the gamete proportions found. These equilibria agreed well with empirical literature data. Conclusion If population differentiation with respect to genotype proportions is really driven by gamete patterns, as strongly suggested by the present study, all-hybrid populations constitute not one, but several

  1. Effect of industrial effluents on the growth and anatomical structures ...

    The authors investigated the impact of industrial effluents from 5 different industrial concerns in Lagos, Nigeria on Okra (Abelmoschus esculentus). During the study, it was observed that these effluents induced detrimental effects on the flowering, fruiting, stem length, leaf width and leaf length of okra. Other parameters ...

  2. Z-scan and optical limiting properties of Hibiscus Sabdariffa dye

    Diallo, A.; Zongo, S.; Mthunzi, P.; Rehman, S.; Alqaradawi, S. Y.; Soboyejo, W.; Maaza, M.

    2014-12-01

    The intensity-dependent refractive index n 2 and the nonlinear susceptibility χ (3) of Hibiscus Sabdariffa dye solutions in the nanosecond regime at 532 nm are reported. More presicely, the variation of n 2, β, and real and imaginary parts of χ (3) versus the natural dye extract concentration has been carried out by z-scan and optical limiting techniques. The third-order nonlinearity of the Hibiscus Sabdariffa dye solutions was found to be dominated by nonlinear refraction, which leads to strong optical limiting of laser.

  3. Hot and cold water infusion aroma profiles of Hibiscus sabdariffa: fresh compared with dried.

    Ramírez-Rodrigues, M M; Balaban, M O; Marshall, M R; Rouseff, R L

    2011-03-01

    Calyxes from the Roselle plant (Hibiscus sabdariffa L.) were used to prepare cold (22 °C for 4 h) and hot (98 °C for 16 min) infusions/teas from both fresh and dried forms. Aroma volatiles were extracted using static headspace SPME and analyzed using GC-MS and GC-O with 2 different columns (DB-5 and DB-Wax). Totals of 28, 25, 17, and 16 volatiles were identified using GC-MS in the dried hot extract (DHE), dried cold extract (DCE), fresh hot extract (FHE), and fresh cold extract (FCE) samples, respectively. In terms of total GC-MS peak areas DHE ≫ DCE > FHE ≫ FCE. Nonanal, decanal, octanal, and 1-octen-3-ol were among the major volatiles in all 4 beverage types. Thirteen volatiles were common to all 4 teas. Furfural and 5-methyl furfural were detected only in dried hibiscus beverages whereas linalool and 2-ethyl-1-hexanol were detected only in beverages from fresh hibiscus. In terms of aroma active volatiles, 17, 16, 13, and 10 aroma active volatiles were detected for DHE, DCE, FHE, and FCE samples, respectively. The most intense aroma volatiles were 1-octen-3-one and nonanal with a group of 4 aldehydes and 3 ketones common to all samples. Dried samples contained dramatically higher levels of lipid oxidation products such as hexanal, nonanal, and decanal. In fresh hibiscus extracts, linalool (floral, citrus) and octanal (lemon, citrus) were among the highest intensity aroma compounds but linalool was not detected in any of the dried hibiscus extracts. Hibiscus teas/infusions are one of the highest volume specialty botanical products in international commerce. The beverage is consumed for both sensory pleasure and health attributes and is prepared a number of ways throughout the world. Although color and taste attributes have been examined, little information is known about its aroma volatiles and no other study has compared extractions from both fresh and dried as well as extraction temperature differences. This is also, apparently, the first study to identify

  4. Fatty Acid Composition of Hibiscus trionum L. (Malvaceae

    Ceyda Sibel Kılıç

    2011-01-01

    Full Text Available The genus Hibiscus plants have different uses, some are used as foods (H. esculenta L., and some species as remedy in traditional medicine (H. sabdariffa L. as well as a colorant for herbal teas. The only species that grows naturally in Turkey is H. trionum L. The plant especially infests soy and corn fields, and therefore it is considered to be a noxious weed. The plant is also found to be a host for Potato Virus Y (PVY and Tomato Spotted Wilt Virus (TSWV. Infestation of the crop fields by this plant shows that it germinates easily. Since it produces many seeds, distribution of the plant is also quiet easy. Though, seed oil yield is low (4.7% since linoleic acid composition of the seed oil is quiet high (67,5%, it can still be used as a source of unsaturated fatty acids. The other major fatty acids are palmitic, oleic and stearic acids in the studied seed oil .

  5. Fullerene-Based Symmetry in Hibiscus rosa-sinensis Pollen

    Andrade, Kleber; Guerra, Sara; Debut, Alexis

    2014-01-01

    The fullerene molecule belongs to the so-called super materials. The compound is interesting due to its spherical configuration where atoms occupy positions forming a mechanically stable structure. We first demonstrate that pollen of Hibiscus rosa-sinensis has a strong symmetry regarding the distribution of its spines over the spherical grain. These spines form spherical hexagons and pentagons. The distance between atoms in fullerene is explained applying principles of flat, spherical, and spatial geometry, based on Euclid’s “Elements” book, as well as logic algorithms. Measurements of the pollen grain take into account that the true spine lengths, and consequently the real distances between them, are measured to the periphery of each grain. Algorithms are developed to recover the spatial effects lost in 2D photos. There is a clear correspondence between the position of atoms in the fullerene molecule and the position of spines in the pollen grain. In the fullerene the separation gives the idea of equal length bonds which implies perfectly distributed electron clouds while in the pollen grain we suggest that the spines being equally spaced carry an electrical charge originating in forces involved in the pollination process. PMID:25003375

  6. The Effects of Cold Stress on Photosynthesis in Hibiscus Plants

    Paredes, Miriam; Quiles, María José

    2015-01-01

    The present work studies the effects of cold on photosynthesis, as well as the involvement in the chilling stress of chlororespiratory enzymes and ferredoxin-mediated cyclic electron flow, in illuminated plants of Hibiscus rosa-sinensis. Plants were sensitive to cold stress, as indicated by a reduction in the photochemistry efficiency of PSII and in the capacity for electron transport. However, the susceptibility of leaves to cold may be modified by root temperature. When the stem, but not roots, was chilled, the quantum yield of PSII and the relative electron transport rates were much lower than when the whole plant, root and stem, was chilled at 10°C. Additionally, when the whole plant was cooled, both the activity of electron donation by NADPH and ferredoxin to plastoquinone and the amount of PGR5 polypeptide, an essential component of the cyclic electron flow around PSI, increased, suggesting that in these conditions cyclic electron flow helps protect photosystems. However, when the stem, but not the root, was cooled cyclic electron flow did not increase and PSII was damaged as a result of insufficient dissipation of the excess light energy. In contrast, the chlororespiratory enzymes (NDH complex and PTOX) remained similar to control when the whole plant was cooled, but increased when only the stem was cooled, suggesting the involvement of chlororespiration in the response to chilling stress when other pathways, such as cyclic electron flow around PSI, are insufficient to protect PSII. PMID:26360248

  7. Hibiscus fiber carbon for fuel cell device material

    Nanik Indayaningsih; Anne Zulfia; Dedi Priadi; Suprapedi

    2010-01-01

    The objective of this research is carbon of hibiscus fibers for the application as basic material of fuel cell device. The carbon is made using a pyrolysis process in inert gas (nitrogen) for 1 hour at temperature of 500 °C, 700 °C and 900 °C. The X-Ray Diffractometer (XRD), Scanning Electron Microscope (SEM) and Impedance-Capacitance-Resistance-meter are used to find out the microstructure, morphology and electrical properties respectively. The results of the experiment showed that the carbon had a structure of amorphous, and as the semiconductor material the electrical conductivity was 5 x 10"-"5 S.cm"-"1 to 4.9 x 10"-"5 S.cm"-"1 increasing in accordance with the pyrolysis temperature. The morphology resembled to plaited mats constructed by porous fibers having width of 50 µm to 300 µm, thickness of 25 µm to 35 µm, and the porous size of 0.5 µm to 5 µm. This morphology enables carbon to be applied as a candidate for a basic material of the Proton Exchange Membrane Fuel Cell. (author)

  8. Comparative chemical and biochemical analysis of extracts of Hibiscus sabdariffa.

    Sindi, Heba A; Marshall, Lisa J; Morgan, Michael R A

    2014-12-01

    Hibiscus sabdariffa extracts have attracted attention because of potentially useful bioactivity. However, there have been no systematic studies of extraction efficiencies of H. sabdariffa. The nature of extracts used in different studies has varied considerably, making comparisons difficult. Therefore, a systematic study of extracts of H. sabdariffa made with different solvents was carried out using water, methanol, ethyl acetate and hexane in the presence/absence of formic acid, using different extraction times and temperatures. The extracts were analysed for total polyphenol content, antioxidant capacity using DPPH, FRAP and TEAC assays, and specific anthocyanins were determined using HPLC and LC-MS. The results showed the highest antioxidant capacities were obtained by extracting using water, with or without formic acid, for 10 min at 100°C. These extracts provided the highest concentrations of cyanidin 3-sambubioside and delphinidin 3-sambubioside. It will be important to use extraction conditions giving optimal extraction efficiencies for subsequent bioactivity experiments. Copyright © 2014 Elsevier Ltd. All rights reserved.

  9. Physicochemical properties and antimicrobial activity of Roselle (Hibiscus sabdariffa L.).

    Jung, EunKyung; Kim, YoungJun; Joo, Nami

    2013-12-01

    The therapeutic action of a plant depends on its chemical constituents. In this study, experiments were carried out in order to evaluate the effect of extraction conditions on the antioxidative and antimicrobial activities of Roselle (Hibiscus sabdariffa L.). Roselle was found to be rich in malic acid, anthocyanins, ascorbic acid and minerals, especially Ca and Fe, but low in glucose. More than 18 volatile compounds were identified by gas chromatography and gas chromatography-mass spectrometry. This herb, which is rich in phenolic compounds and displays DPPH radical scavenging activity, could be a good source of natural antioxidants. The antimicrobial activity of the Roselle water and ethanol extracts was tested with Bacillus subtilis (ATCC6633), Staphylococcus aureus (ATCC6538) and Escherichia coli (ATCC 8739). The inhibition of the Roselle ethanol extract against B. subtilis and S. aureus was slightly higher than that of water extract but this difference was not significant. However, E. coli was strongly inhibited by the Roselle water extract at concentrations of 25 and 50 mg mL(-1) as determined by a paper disc method. The obtained results indicated that antioxidant and antimicrobial activity was related to different methods of extraction and Roselle extracts could be a source of therapeutically useful products. © 2013 Society of Chemical Industry.

  10. Wound Healing Potential of Formulated Extract from Hibiscus Sabdariffa Calyx

    Builders, P. F.; Kabele-Toge, B.; Builders, M.; Chindo, B. A.; Anwunobi, Patricia A.; Isimi, Yetunde C.

    2013-01-01

    Wound healing agents support the natural healing process, reduce trauma and likelihood of secondary infections and hasten wound closure. The wound healing activities of water in oil cream of the methanol extract of Hibiscus sabdariffa L. (Malvaceae) was evaluated in rats with superficial skin excision wounds. Antibacterial activities against Pseudomonas aeroginosa, Staphylococcus aureus and Echerichia coli were determined. The total flavonoid content, antioxidant properties and thin layer chromatographic fingerprints of the extract were also evaluated. The extract demonstrated antioxidant properties with a total flavonoid content of 12.30±0.09 mg/g. Six reproducible spots were obtained using methanol:water (95:5) as the mobile phase. The extract showed no antimicrobial activity on the selected microorganisms, which are known to infect and retard wound healing. Creams containing H. sabdariffa extract showed significant (Psabdariffa extract. This study, thus, provides evidence of the wound healing potentials of the formulated extract of the calyces of H. sabdariffa and synergism when co-formulated with gentamicin. PMID:23901160

  11. Ethylene: Response of Fruit Dehiscence to CO(2) and Reduced Pressure.

    Lipe, J A; Morgan, P W

    1972-12-01

    These studies were conducted to determine whether ethylene serves as a natural regulator of fruit wall dehiscence, a major visible feature of ripening in some fruits. We employed treatments to inhibit ethylene action or remove ethylene and observed their effect on fruit dehiscence. CO(2) (13%), a competitive inhibitor of ethylene action in many systems, readily delayed dehiscence of detached fruits of cotton (Gossypium hirsutum L.), pecan (Carya illinoensis [Wang.] K. Koch), and okra (Hibiscus esculentus L.). The CO(2) effect was duplicated by placing fruits under reduced pressure (200 millimeters mercury), to promote the escape of ethylene from the tissue. Dehiscence of detached fruits of these species as well as attached cotton fruits was delayed. The delay of dehiscence of cotton and okra by both treatments was achieved with fruit harvested at intervals from shortly after anthesis until shortly before natural dehiscence. Pecan fruits would not dehisce until approximately 1 month before natural dehiscence, and during that time, CO(2) and reduced pressure delayed dehiscence. CO(2) and ethylene were competitive in their effects on cotton fruit dehiscence. All of the results are compatible with a hypothetical role of ethylene as a natural regulator of dehiscence, a dominant aspect of ripening of cotton, pecan, and some other fruits.

  12. Ethylene: Response of Fruit Dehiscence to CO2 and Reduced Pressure 1

    Lipe, John A.; Morgan, Page W.

    1972-01-01

    These studies were conducted to determine whether ethylene serves as a natural regulator of fruit wall dehiscence, a major visible feature of ripening in some fruits. We employed treatments to inhibit ethylene action or remove ethylene and observed their effect on fruit dehiscence. CO2 (13%), a competitive inhibitor of ethylene action in many systems, readily delayed dehiscence of detached fruits of cotton (Gossypium hirsutum L.), pecan (Carya illinoensis [Wang.] K. Koch), and okra (Hibiscus esculentus L.). The CO2 effect was duplicated by placing fruits under reduced pressure (200 millimeters mercury), to promote the escape of ethylene from the tissue. Dehiscence of detached fruits of these species as well as attached cotton fruits was delayed. The delay of dehiscence of cotton and okra by both treatments was achieved with fruit harvested at intervals from shortly after anthesis until shortly before natural dehiscence. Pecan fruits would not dehisce until approximately 1 month before natural dehiscence, and during that time, CO2 and reduced pressure delayed dehiscence. CO2 and ethylene were competitive in their effects on cotton fruit dehiscence. All of the results are compatible with a hypothetical role of ethylene as a natural regulator of dehiscence, a dominant aspect of ripening of cotton, pecan, and some other fruits. PMID:16658260

  13. Effet de l'incorporation de graines d' Hibiscus Sabdariffa (Oseille de ...

    Effet de l'incorporation de graines d' Hibiscus Sabdariffa (Oseille de Guinée) dans l'alimentation sur quelques paramètres d'ingestion et de croissance du poulet en aviculture traditionnelle améliorée au Burkina Faso.

  14. Toxicity studies of the water extract from the calyces of Hibiscus ...

    Acute and chronic toxicities of the water extract from calyces of Hibiscus sabdariffa were studied in male and female rats. After 14 days of a single oral administration of test substance 5,000 mg/kg body weight, measurement of the body and organ weights, necropsy and health monitoring were performed. No signs and ...

  15. Effect of methanolic extract of Hibiscus sabdariffa in ethanol-induced ...

    The objective of this study was to evaluate the activity of Hibiscus sabdariffa on the liver of rats following repeated administration of ethanol. Hepatotoxicity was induced on the rats using ethanol and the levels of serum enzymes such as serum glutamic pyruvic transaminase (SGPT), serum glutamic oxaloacetic transaminase ...

  16. Effect of rosella ( Hibiscus sabdariffa L ) extract on glutathione-S ...

    Purpose: To determine the effect of rosella (Hibiscus sabdariffa L) extract on glutathione-S-trasferase (GST) activity and its hepatoprotective effect. Methods: A total of 25 rats were divided randomly into 5 groups (5 rats per group). Group I served as the baseline, group II was the negative control group, while groups III, IV and ...

  17. Phytomining of heavy metals from soil by Hibiscus radiatus using phytoremediationtechnology (Part-2)

    Panchal, K. J.; Subramanian, R. B.; Gohil, T. P.

    2017-12-01

    Metal ions are not only valuable intermediates in metal extraction, but also important raw materials fortechnical applications. They possess some unique but, identical physical and chemical properties, whichmake them useful probes of low temperature geochemical reactions. Heavy metals are natural constituentsof the earth's crust, but indiscriminate human activities have drastically altered their geochemical cyclesand biochemical balance. Metal concentration in soil typically ranges from less than one to as high as100,000 mg/kg. Heavy metal contaminations of land resources continue to be the focus of numerousenvironmental studies and attract a great deal of attention worldwide. This is attributed to nobiodegradabilityand persistence of heavy metals in soils. Prolonged exposure to heavy metals such ascadmium, copper, lead, nickel, and zinc can cause deleterious health effects in humans. Complexation,separation, and removal of metal ions have become increasingly attractive areas of research and have ledto new technical developments like phytoremediation that has numerous biotechnological implications ofunderstanding of plant metal accumulation. Hibiscus radiatus is newly identified as a potential heavymetal hypreaccumulator. In this study Hibiscus radiatus was subjected for in vitro heavy metalaccumulation, to explore the accumulation pattern of four heavy metals viz Cadmium, Lead, Nickel andZinc in various parts of Hibiscus radiatus plant parts. Translocation of metals in Hibiscus radiatus plant parts from soil makes this plant an eligible candidate to remove heavy metals from soil.

  18. Composition of the seed oil of Hibiscus abelmoschus L. (Malvaceae) growing in Vietnam

    Dung, N.X.; Khien, P.V.; Nhuan, D.D.; Hoi, T.M.; Ban, N.K.; Leclercq, P.A.; Muselli, A.; Bighelli, A.; Casanova, J.

    1999-01-01

    The essential oils of ambrette (Hibiscus abelmoschus 1., syn. Abelmoschus moschatus) were produced from seeds collected in the different provinces of Vietnam. Five samples were investigated and 35 components were identified by a combination of high resolution GC, GC/MS and 13C-NMR spectrometry. The

  19. Kenaf (Hibiscus cannabinus L.) impact on post-germination seedling growth

    The chemical interaction between plants, which is referred to as allelopathy, may result in the inhibition of plant growth and development. The objective of this research was to determine the impact of kenaf (Hibiscus cannabinus L.) plant extracts on the post-germination growth of five plant species...

  20. Molecular characterization of ferulate 5-hydroxylase gene from kenaf (Hibiscus cannabinus L.)

    The purpose of this research was to clone and characterize the expression pattern of a kenaf (Hibiscus cannabinus L.) F5H gene that encodes ferulate 5-hydroxylase in the phenylpropanoid pathway. Kenaf is well known as a fast growing dicotyledonous plant, which makes it a valuable biomass plant. The ...

  1. ‘Hapa White’, 'Hapa Pink', and 'Hapa Red' interspecific hybrid hibiscus cultivars

    Hibiscus mutabilis, also known as confederate rose, is native to southeastern China, but it is also grown as an ornamental throughout the southeastern United States and is hardy in USDA zone 7 to 9. It is popular for its large, soft, gray-green foliage during the summer, and large, showy flowers pro...

  2. Effects of Hibiscus sabdariffa L. on serum lipids: a systematic review and meta-analysis.

    Aziz, Zoriah; Wong, Su Yuen; Chong, Nyuk Jet

    2013-11-25

    Prevention of cardiovascular disease by modifying its major risk factors, including serum cholesterol levels, is an important strategy. Hibiscus sabdariffa L. has been promoted for reducing cholesterol levels, but its reported impact on cholesterol levels has been inconsistent. The study aimed to assess systematically the evidence and quality of current research on the effect of Hibiscus sabdariffa L. on blood lipids and its adverse effects. Electronic databases were searched up to June 2013 for relevant randomised controlled trials (RCTs). Journals and conference proceedings were also searched. The quality of the selected trials was assessed using the Cochrane Risk of Bias Assessment Tool. The efficacy results of similar studies were pooled if they used the same comparator. Outcomes examined were levels of total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol and triglycerides. Six studies involving 474 subjects met our inclusion criteria. These studies varied in terms of the types of interventions, comparators used, and duration of trials. Overall, Hibiscus sabdariffa L. did not produce any significant effect on any of the outcomes examined, when compared with placebo, black tea or diet. With short-term use it is well tolerated. The available evidence from RCTs does not support the efficacy of Hibiscus sabdariffa L. in lowering serum lipids. Further rigorously designed trials with larger sample sizes are warranted to confirm the effects of HS on serum lipids. © 2013 Elsevier Ireland Ltd. All rights reserved.

  3. Cytotoxic Activity of the Leaf and Stem Extracts of Hibiscus rosa ...

    Methods: The crude petroleum ether, ethyl acetate and methanol extracts of the leaf and stem of Hibiscus rosa sinensis were prepared using cold extraction method. The in vitro cytotoxic activity of the extracts (20 - 100 μg/ml) was evaluated on leukaemic cancer cell line (K-562) and Mardin-Darby kidney cell line (MDBK) ...

  4. Micro-element contents of roselle ( Hibiscus sabdariffa L.) at different ...

    In the western Sahel, leaves of Roselle (Hibiscus sabdariffa) have considerable economic importance because of their nutritional and medical uses. These plant organs are used to supplement nutrients provided by cereals such as millet and sorghum. However, there is a lack of information on the nutrient composition of ...

  5. In vivo pharmacodynamic and pharmacokinetic interactions of Hibiscus sabdariffa calyces extracts with simvastatin.

    Showande, S J; Adegbolagun, O M; Igbinoba, S I; Fakeye, T O

    2017-12-01

    Increasing number of patients use herbs with their medications. Such practice may result in beneficial or harmful herb-drug interactions. A recent survey reported that some participants co-administered Hibiscus sabdariffa, a widely used beverage, or tea, with their antihyperlipidaemic medications. This study therefore evaluated the effect of concomitant administration of Hibiscus sabdariffa calyces' extracts with simvastatin on hyperlipidaemia and pharmacokinetics of the drug in vivo. Factorial experimental designs were used to evaluate the comparative effectiveness and interactions between simvastatin and aqueous extract of Hibiscus sabdariffa (AEHS) on lipid profile parameters in hyperlipidaemia-induced Wistar rats. Different combinations of low (AEHS 250 mg/kg; simvastatin 10 mg/kg) and high doses (AEHS 500 mg/kg; simvastatin 20 mg/kg) were administered individually and concurrently daily for 2 and 4 weeks. Lipid profile parameters were assessed at these treatment periods. Subsequently, the effect of aqueous beverage of Hibiscus sabdariffa (ABHS) on the pharmacokinetics of single-dose 40 mg simvastatin was also evaluated in six healthy human volunteers using two-period randomized crossover design. Blood samples were collected at predetermined times for 24 hours. The plasma obtained was analysed for simvastatin using RP-HPLC/UV method. Aqueous extract of Hibiscus sabdariffa reduced total cholesterol (T c ) better than simvastatin (P = .031). Low-dose AEHS and low-dose simvastatin used concomitantly caused 38.3% and 57.4% reductions in T c and triglyceride levels, respectively, compared with low-dose simvastatin (P Hibiscus sabdariffa lowered T c better than simvastatin and enhanced the antihyperlipidaemic activity of the drug when co-administered at low doses in an animal model. However, aqueous beverage of Hibiscus sabdariffa caused a significant herb-drug interaction resulting in overall reduction in exposure to simvastatin in humans. Caution should

  6. Evaluation of roselle (Hibiscus sabdariffa) leaf and pomegranate (Punica granatum) fruit rind for activity against Meloidogyne incognita

    Pomegranate (Punica granatum) fruit and roselle (Hibiscus sabdariffa) leaves have been used in traditional medicine, including as anthelmintics. Methanolic extracts from these plants were investigated for activity against the southern root-knot nematode (RKN) Meloidogyne incognita. Dried, ground p...

  7. Quercetin, kaempferol, myricetin, and fatty acid content among several Hibiscus sabdariffa accession calyces based on maturity in a greenhouse

    Flavonols including quercetin, kaempferol, myricetin, and fatty acids in plants have many useful health attributes including antioxidants, cholesterol lowering, and cancer prevention. Six accessions of roselle, Hibiscus sabdariffa calyces were evaluated for quercetin, kaempferol, and myricetin conte...

  8. Efek Hepatoprotektif Rosella (Hibiscus sabdariffa L. pada Tikus Model Hepatitis

    Ike Rostikawati Husen

    2012-06-01

    Full Text Available Hepatitis is initiated by pollutant which caused oxidative stress. Rosella (Hibiscus sabdariffa L. contain flavonoid, an antioxidant which has been used traditionally in treating liver disease. The study aims to define the hepatoprotective effects of fresh rosella calyx ethanol extract (FRCEE on hepatitis model rats with liver malondialdehyde (MDA and serum glutamic piruvic transaminase (SGPT level and hepatocyte morphology damage compared to vitamin E’s effects. The laboratory experimental study has been conducted on August 2009 at Department Pharmacology and Therapy Faculty of Medicine Padjadjaran University/Dr. Hasan Sadikin Hospital in 25 male Wistar rats, divided into 5 groups. Group 1 was negative control, group 2 was positive control (carbon tetrachloride/CCl4-hepatitis model rats, group 3 and 4 were hepatitis model rats that subsequently given 125 mg/kgBW of FRCEE (group 3 and 2.7 IU of vitamin E (group 4 for 8 days; group 5 was given FRCEE. Observations were done 48 hours after CCL4 induction. The results were analyzed by analysis of varian (ANOVA and Tukey. The result showed that FRCEE inhibited increasing of liver MDA and SGPT level significantly different compared to positive control (MDA p=0.00, SGPT p=0.041 but not significantly different to group 4 (MDA p=1.00, SGPT p=0.192. Histological features showed macrovesicular on group 3, microvesicular vacuole on group 2; whereas vitamin E inhibited morphological damage. In conclusion, FRCEE has hepatoprotective effect on hepatitis model rats as antioxidant, but not superior compared to vitamin E.

  9. INDIKATOR TITRASI ASAM-BASA DARI EKSTRAK BUNGA SEPATU (Hibiscus rosa sinensis L Indicator of Acid-Base Titration from the Extract of Hibiscus rosa sinensis L Flower

    Siti Nuryanti

    2012-05-01

    Full Text Available Titration acid-base needs indicator  to show the change of color on interval of hydrogen exponent/degree of acid (pH. Indicator of synthetic which always be used have disadvantages like chemical pollution,  stock and expensive of pro- duction  cost. The research has been carried out to substitute the synthetic indicator with herbal indicator extracted from flower crown of Hibiscus rosa sinensis L extract. The herbal indicator was extracted from the flower crown Hibiscus rosa sinensis L using a mixture methanol-acetic acid. Then it was evaluated with phenolphthalein and methyl orange(E merck comparer to titration the acid-base, they are strong acid-strong base, weak base-strong acid and weak acid-strong base. The result of research show that herbal indicator  of flower crown Hibiscus rosa sinensis L to show theequivalent point in all titrations give peer result with the comparison. With the research result hoped that indicatortitration acid-base flower crown Hibiscus rosa sinensis L is able to as replace synthetic indicator  (metyl orange andphenolphtalein which always be used before. ABSTRAK Titrasi asam-basa memerlukan indikator untuk menunjukkan perubahan warna pada setiap interval derajad keasaman (pH. Indikator sintetis yang digunakan selama ini mempunyai beberapa kelemahan seperti polusi kimia, ketersediaan dan biaya produksi mahal. Upaya penelitian sudah dilakukan untuk menggantikan indikator sintetis dengan indikator dari ekstrak mahkota bunga sepatu. Indikator herbal tersebut dibuat dengan cara mengekstrak mahkota bunga Hibiscus rosa sinensis L dengan mengunakan pelarut metanol-asam asetat. Kemudian dievaluasi dengan indikator pembanding fenolftalein dan metil oranye (produksi E merck untuk titrasi asam-basa yaitu asam kuat-basa kuat, basa lemah-asam kuat dan asam lemah-basa kuat. Dari hasil penelitian diketahui bahwa indikator dari mahkota bunga sepatu untuk menunjukkan titik ekivalen dalam titrasi tersebut memberikan hasil yang

  10. Diuretic effect of compounds from Hibiscus sabdariffa by modulation of the aldosterone activity.

    Jiménez-Ferrer, Enrique; Alarcón-Alonso, Javier; Aguilar-Rojas, Arturo; Zamilpa, Alejandro; Jiménez-Ferrer C, Itzia; Tortoriello, Jaime; Herrera-Ruiz, Maribel

    2012-12-01

    Recent studies of Hibiscus sabdariffa Linn. have demonstrated that it presents diuretic, natriuretic, and potassium sparing effects. However, the mechanism that induces these effects has not yet been elucidated. The aim of this study was to explore the possible mechanism of action for the diuretic effect of Hibiscus sabdariffa extract and its fractions.The aqueous extract from this plant and the fractions obtained with solvents of different polarities were administered to adrenalectomized rats, and the diuretic effect was measured in the presence of deoxycorticosterone acetate (aldosterone analog).The effect on renal filtration was also evaluated in an in situ kidney model, and finally, the effect of diuretic active extracts on gene expression of the alpha subunit from the transporter (αENaC) of renal epithelial cell was quantified. The subsequent results were obtained: The aqueous extract of Hibiscus sabdariffa presented the following chemical composition, 32.4 mg/g delphinidin-3-O-sambubioside, 11.5 mg/g cyanidin-3-O-sambubioside, 11.5 mg/g quercetin, and chlorogenic acid 2.7 mg/g. The concentration of anthocyanins was diminished until disappearance due to decrease of the polarity of the solvents used in the extraction process, in contrast to the flavonoids and chlorogenic acid, which had their concentration increased. The diuretic effect caused by adrenalectomy in rats was reversed by deoxycorticosterone acetate activity. However, the effect of deoxycorticosterone acetate was antagonized by spironolactone, the aqueous extract of Hibiscus sabdariffa, and the acetonitrile : methanol 5 : 5 mixture extract, administered orally. A similar effect was observed on renal filtration obtained from the isolated kidney model.When the gene expression levels of αENaC was measured in adrenalectomized rats, it was observed that spironolactone, the aqueous extract of Hibiscus sabdariffa, the acetonitrile : methanol 5 : 5 mixture, as well as the

  11. Sphingomonas rhizophila sp. nov., isolated from rhizosphere of Hibiscus syriacus.

    Yan, Zheng-Fei; Lin, Pei; Won, Kyung-Hwa; Li, Chang-Tian; Park, GyungSoo; Chin, ByungSun; Kook, MooChang; Wang, Qi-Jun; Yi, Tae-Hoo

    2018-02-01

    A Gram-stain-negative, aerobic, non-motile, rod-shaped, catalase-positive and oxidase-positive bacteria (THG-T61 T ), was isolated from rhizosphere of Hibiscus syriacus. Growth occurred at 10-37 °C (optimum 25-30 °C), at pH 5.0-9.0 (optimum 7.0) and in the presence of 0-2.0 % NaCl (optimum without NaCl supplement). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T61 T were identified as Sphingomonas ginsengisoli KCTC 12630 T (97.9 %), Sphingomonas jaspsi DSM 18422 T (97.8 %), Sphingomonas astaxanthinifaciens NBRC 102146 T (97.4 %), Sphingomonassediminicola KCTC 12629 T (97.2 %), 'Sphingomonas swuensis' KCTC 12336 (97.1 %) and Sphingomonas daechungensis KCTC 23718 T (96.9 %). The isoprenoid quinone was ubiquinone-10 (Q-10). The major fatty acids were C16 : 0, C17 : 1ω6c, summed feature 4 (iso-C15 : 0 2-OH and/or C16 : 1ω7c) and summed feature 7 (C18 : 1ω7c, C18 : 1ω9t and/or C18 : 1ω12t). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid, one unidentified lipid, one unidentified phospholipid, one unidentified glycolipid and one unidentified phosphoglycolipid. The polyamine was homospermidine. The DNA G+C content of strain THG-T61 T was 65.6 mol%. The DNA-DNA relatedness values between strain THG-T61 T and its closest reference strains were less than 49.2 %, which is lower than the threshold value of 70 %. Therefore, strain THG-T61 T represents a novel species of the genus Sphingomonas, for which the name Sphingomonas rhizophila sp. nov. is proposed. The type strain is THG-T61 T (=KACC 19189 T =CCTCC AB 2016245 T ).

  12. Chemical composition of some Roselle (Hibiscus sabdariffa) genotypes

    Alshoosh, Wafa Gamal Abdalla

    1997-10-01

    Six lines of of Hibiscus sabdariffa were used in this study. The proximate analysis gave moisture 6.19%, 12.07% and 8.42%, ash 10.6%, 7.98% and 12.42%, protein 9.76%, 5.53% and 6.13%, oil 0.99%, 0.95% and 0.90%, fibre 10.74%, 11.73% and 12.17%, carbohydrate 61.64%, 61.76% and 60.43% for seasons 93/94, 94/95 and 95/96 respectively. Physicochemical investigation of karkade calices gave the following results: reducing sugar 1.29%. 1.28% and 1.27%, non reducing sugar 0.87, 0.77 and 0.86, PH 3.1, 2.71 and 3.001, colour intensity 0.77, 0.72 and 0.51, insoluble solids 37.74%, 36.36% and 37.33% for seasons 93/94, 94/95 and 95/96 respectively. The value of vitamin C and acidity (organic acids) in season 93/94 was 92.06 mg/100g and 19.58% respectively. Organic acids as hibiscic acid, citric acid, vitamin C and xalic acid was 25.51%, 14.15%, 92.69 mg/100g and 0.55% and 26.18%, 14.14%, 97.26 mg/100g and 0.56% for season 94/95 and 95/96 respectively. Mineral contents for karkade calices were measured by using atomic absorption spectroscopy, the results for season 94/95 were 0.56%, 0.24%, 0.08%, 0.23%, 0.95% and 0.19% for Ca, Mg, Fe, Na, K and P respectively, while mineral content for season 95/96, 1.9%, 0.31%, 0.07%, 0.31%, 0.48%, 0.23%. 0.05%, 0.22%, 0.06%, 0.006%, 0.02%, and 0.03% for Ca, Mg, Fe, Na, K, P, Zn, M, Cu, Pb, Co and Cr respectively. UV-visible spectrum showed the presence of the following pigments: dephinidin-3-sambubioside and cyanidin-3-sambuioside. Total anthocyanin of karkade calices were found to be 0.89 to 1.4% and 0.99 to 1.6% for season 94/95 and 95/96 respectively. Acid hydrolysis of the pigments showed the presence of xylose and glucose in addition to the anthocyanidins delphinidin ad cyanidin.(Author)

  13. Chemical composition of some Roselle (Hibiscus sabdariffa) genotypes

    Alshoosh, Wafa Gamal Abdalla [Department of food science and technology, Faculty of Agriculture, University of Khartoum, Khartoum (Sudan)

    1997-10-01

    Six lines of of Hibiscus sabdariffa were used in this study. The proximate analysis gave moisture 6.19%, 12.07% and 8.42%, ash 10.6%, 7.98% and 12.42%, protein 9.76%, 5.53% and 6.13%, oil 0.99%, 0.95% and 0.90%, fibre 10.74%, 11.73% and 12.17%, carbohydrate 61.64%, 61.76% and 60.43% for seasons 93/94, 94/95 and 95/96 respectively. Physicochemical investigation of karkade calices gave the following results: reducing sugar 1.29%. 1.28% and 1.27%, non reducing sugar 0.87, 0.77 and 0.86, PH 3.1, 2.71 and 3.001, colour intensity 0.77, 0.72 and 0.51, insoluble solids 37.74%, 36.36% and 37.33% for seasons 93/94, 94/95 and 95/96 respectively. The value of vitamin C and acidity (organic acids) in season 93/94 was 92.06 mg/100g and 19.58% respectively. Organic acids as hibiscic acid, citric acid, vitamin C and xalic acid was 25.51%, 14.15%, 92.69 mg/100g and 0.55% and 26.18%, 14.14%, 97.26 mg/100g and 0.56% for season 94/95 and 95/96 respectively. Mineral contents for karkade calices were measured by using atomic absorption spectroscopy, the results for season 94/95 were 0.56%, 0.24%, 0.08%, 0.23%, 0.95% and 0.19% for Ca, Mg, Fe, Na, K and P respectively, while mineral content for season 95/96, 1.9%, 0.31%, 0.07%, 0.31%, 0.48%, 0.23%. 0.05%, 0.22%, 0.06%, 0.006%, 0.02%, and 0.03% for Ca, Mg, Fe, Na, K, P, Zn, M, Cu, Pb, Co and Cr respectively. UV-visible spectrum showed the presence of the following pigments: dephinidin-3-sambubioside and cyanidin-3-sambuioside. Total anthocyanin of karkade calices were found to be 0.89 to 1.4% and 0.99 to 1.6% for season 94/95 and 95/96 respectively. Acid hydrolysis of the pigments showed the presence of xylose and glucose in addition to the anthocyanidins delphinidin ad cyanidin.(Author) 90 refs. , 18 tabs. , 14 figs.

  14. Growth, Yield and WUE of Drip and Sprinkler Irrigated Okra Grown On Sandy Soil Under Semi-Arid Conditions in Southeast Ghana

    Plauborg, Finn

    Vegetable production systems at the Keta sand spit, Southeast Ghana, are typically managed with excessive amounts of irrigation water and fertilizers on sandy soils with low inherent water and nutrient retention capacities. The shallow groundwater which is the primary irrigation water resource...... is prone to salinization from the Keta lagoon, the Atlantic Ocean and brackish water underneath (Kortatsi and Agyeku, 1999). To ensure the sustainability of vegetable production at the Keta spit, introduction of water saving irrigation systems and improved irrigation management schemes are important. Thus......, the main aim of our study was to explore the water sa ving potential of drip irrigation in order to save the shallow groundwater from over exploitation. A two season study (minor dry season, 2011 and major dry season, 2012) were carried out to determine the okra crop response to the following treatments: 1...

  15. Effect of different fertilization and irrigation methods on nitrogen uptake, intercepted radiation and yield of okra (Abelmoschus esculentum L.) grown in the Keta Sand Spit of Southeast Ghana

    Danso, E. Oppong; Abenney-Mickson, S.; Sabi, E.B.

    2015-01-01

    spread, (ii) irrigation by sprinkler, fertilized with localized manure, (iii) irrigation by drip, fertilized with localized manure, (iv) irrigation by drip, fertigated with N-K chemical fertilizers (twice during the crop season in the first experiment, weekly in the second and third experiment). Nitrogen...... uptake, crop interception of solar radiation, yield and water productivity were compared among treatments. The crop did not respond well when fertigation was done only twice, probably due to N-leaching. However, in the second and third experiments, when fertigation was done weekly for eight weeks......Three seasons' experiments were conducted in the Keta Sand Spit to test if current use of sprinkler irrigation and animal manure can be substituted by water saving drip fertigation with reduced P supply to okra. The treatments compared were: (i) irrigation by sprinkler, fertilized with manure...

  16. Lipophilization and MS characterization of the main anthocyanins purified from hibiscus flowers.

    Grajeda-Iglesias, Claudia; Salas, Erika; Barouh, Nathalie; Baréa, Bruno; Figueroa-Espinoza, Maria Cruz

    2017-09-01

    Hibiscus sabdariffa flowers represent an interesting source of anthocyanins, one of the most important plant pigments, which are responsible of the intense red color of the calyces, and have potential as natural colorants for food applications. Nevertheless, anthocyanins are highly hydrosoluble and unstable compounds. On this basis, the aim of this work was to increase the lipophilicity of the hibiscus anthocyanins by lipophilization, in order to obtain amphiphilic colorants, which could be easily incorporated in lipid-rich food matrices. Octanoyl derivatives of delphinidin-3-O-sambubioside and cyanidin-3-O-sambubioside were chemically obtained for the first time, and characterized by means of HPLC-ESI-MS data. Copyright © 2017. Published by Elsevier Ltd.

  17. Phytochemical analysis of Hibiscus caesius using high performance liquid chromatography coupled with mass spectrometry.

    Ain, Quratul; Naveed, Muhammad Na; Mumtaz, Abdul Samad; Farman, Muhammad; Ahmed, Iftikhar; Khalid, Nauman

    2015-09-01

    Various species in genus Hibiscus are traditionally known for their therapeutic attributes. The present study focused on the phytochemical analysis of a rather unexplored species Hibiscus caesius (H. caesius), using high-pressure liquid chromatography coupled with mass spectrometry (HPLC-MS). The analysis revealed five major compounds in the aqueous extract, viz. vanillic acid, protocatechoic acid, quercetin, quercetin glucoside and apigenin, being reported for the first time in H. caesius. Literature suggests that these compounds have important pharmacological traits such as anti-cancer, anti-inflammatory, anti-bacterial and hepatoprotective etc. however, this requires further pharmacological investigations at in vitro and in vivo scale. The above study concluded the medicinal potential of H. caesius.

  18. Destruction of the Phoenix/Hibiscus and Barringtonia racemosa Communities at Richards Bay, Natal, South Africa

    P. J. Weisser

    1982-10-01

    Full Text Available The destruction of the Phoenix!Hibiscus and Barringtonia racemosa Communities described by Venter in 1972 on the southern shores of Richards Bay is reported. The cause was the artificial openingof a new mouth about 5,5 km south of the original mouth, which increased tidal range and salinity. These swamp communities occupied a narrow band about 6 ha in area behind the Bruguiera gymnorrhiza Community. An estimated 95 % of the communities was affected and only on the landward border were some isolated remnants of species such as Acrostichum aureum, Hibiscus tiliaceus and Phoenix reclinata detected .Young stands of  Phragmites australis, seedlings of  Bruguiera gymnorrhiza and Avicennia marina and epipelic algae are recoIonizing the affected area.

  19. Effects of water extract of Hibiscus sabdariffa, Linn (Malvaceae) 'Roselle' on excretion of a diclofenac formulation.

    Fakeye, T O; Adegoke, A O; Omoyeni, O C; Famakinde, A A

    2007-01-01

    The effect of beverages prepared from the dried calyx of the flowers of Hibiscus sabdariffa on the excretion of diclofenac was investigated using a controlled study in healthy human volunteers. A high pressure liquid chromatographic method was used to analyse the 8 h urine samples collected after the administration of diclofenac with 300 mL (equivalent to 8.18 mg anthocyanins) of the beverage administered daily for 3 days. An unpaired two-tailed t-test was used to analyse for significant difference observed in the amount of diclofenac excreted before and after administration of the beverage. There was a reduction in the amount of diclofenac excreted and the wide variability observed in the control with the water beverage of Hibiscus sabdariffa (p < 0.05). There is an increasing need to counsel patients against the use of plant beverages with drugs.

  20. The South American species of Hibiscus sect. Furcaria DC. (Malvaceae-Hibisceae

    Antonio Krapovickas

    2004-01-01

    Full Text Available The Hibiscus section Furcaria from South America is revised. Ten new species from Brasil are described: H. Andersonii, H. capitalensis, H. chapadensis, H. Gregoryi, H. Hochreutineri, H. itirapinensis, H. matogrossensis, H. Nanuzae, H. Saddii, H. Windischii, and a new one from Perú: H. Chancoae. Two new names are proposed: H. Hilarianus from Brasil and H. amambayensis from Paraguay. A key is provided to distinguish the 40 species of section Furcaria known from South America

  1. Antihyperlipidemic activity of Hibiscus rosa-sinensis Linn. ethanolic extract fractions

    Mukesh Singh Sikarwar; M B Patil

    2015-01-01

    Aim: The study investigates the antihyperlipidemic effect of Hibiscus rosa-sinensis Linn. (Malvaceae) ethanolic extract fractions in triton and atherogenic diet-induced hyperlipidemic rats. Materials and Methods: Oral administrations of 500 mg/kg body weight of various fractions of selected plant were evaluated for possible antihyperlipidemic activity in triton and atherogenic diet-induced hyperlipidemic rats for duration of 48 h and 14 days respectively. In triton model, hyperlipidemia was i...

  2. Functional properties of roselle (Hibiscus sabdariffa L.) seed and its application as bakery product

    Nyam, Kar-Lin; Leao, Sod-Ying; Tan, Chin-Ping; Long, Kamariah

    2012-01-01

    Roselle (Hibiscus sabdariffa L.) seed is a valuable food resource as it has an excellent source of dietary fibre. Therefore, this study examined the functional properties of roselle seeds. Replacement of cookie flour with roselle seed powder at levels of 0–30 % was investigated for its effect on functional and nutritional properties of cookies. Among the four formulations cookies, the most preferred by panelists was 20 % roselle seed powder cookie (F3), followed by 10 % roselle seed powder co...

  3. Occurrence of Root Rot and Vascular Wilt Diseases in Roselle (Hibiscus sabdariffa L.) in Upper Egypt

    Hassan, Naglaa; Shimizu, Masafumi; Hyakumachi, Mitsuro

    2014-01-01

    Roselle (Hibiscus sabdariffa L.) family Malvaceae is an important crop used in food, cosmetics and pharmaceutics industries. Roselle is cultivated mainly in Upper Egypt (Qena and Aswan governorates) producing 94% of total production. Root rot disease of roselle is one of the most important diseases that attack both seedlings and adult plants causing serious losses in crop productivity and quality. The main objective of the present study is to identify and characterize pathogens associated wit...

  4. Evaluation of various physico-chemical properties of Hibiscus sabdariffa and L. casei incorporated probiotic yoghurt.

    Rasdhari, M; Parekh, T; Dave, N; Patel, V; Subhash, R

    2008-09-01

    The present investigation was carried out to examine the effect of Hibiscus sabdariffa Calyx extract on the physico-chemical properties, sensory attributes, texture and microbial analysis of L. casei incorporated in probiotic yoghurt after manufacture and during storage. Incorporation of Hibiscus sabdariffa Calyx extract into the probiotic yoghurt resulted into decrease in coagulation time by 25 min. The pH ranged from 4.39 to 4.59, TA 0.81 to 1.14%, moisture 3.05 to 3.37 g%, syneresis 18.85 to 24.90 mL/50 g of sample, % inhibition 12.32 to 59.43, TS 21.27 to 24.90 g% and beta-galactosidase activity 1.041 to 3.277. The protein content ranged between 4.11 and 4.14 g% while the fat content ranged between 3.43 and 3.49 g%. No major changes in sensory evaluation were observed on the day of manufacture and during storage for 7 days. Sabdariffa added yoghurt showed a higher score in almost all sensory attributes. Microbial analysis showed a total plate count ranging from 1.8 x 10(4) to 1.85 x 10(7) cfu mL(-1). Yeast and mold counts were negligible in the Sabdariffa yoghurts. Thus the study concludes that incorporation of Hibiscus sabdariffa extract in yoghurt improved the total antioxidant property, organoleptic qualities and decreased the exudation of whey proteins (Syneresis). Thus, Hibiscus sabdariffa Calyces has beneficial influence on the quality of L. casei incorporated probiotic yoghurt.

  5. Synthesis, Characterization and Evaluation of the Transformations in Hibiscus sabdariffa-graft-poly(butyl acrylate

    B. S. Kaith

    2008-01-01

    Full Text Available Different reaction parameters for the graft copolymerization of butyl acrylate onto Hibiscus sabdariffa fiber were optimized. Graft copolymers thus obtained were subjected to characterization using XRD, TGA, DTA, SEM and FTIR techniques and were evaluated for physio-chemical changes in the behavior. The percentage crystallinity and crystallinity index were found to decrease with increase in grafting while there was reduction in moisture absorption and increase in chemical, thermal resistance of the graft copolymers.

  6. OPTIMIZATION OF HIBISCUS SABDARIFFA L. (ROSELLE) ANTHOCYANIN AQUEOUS-ETHANOL EXTRACTION PARAMETERS USING RESPONSE SURFACE METHODOLOGY

    ANILÚ MIRANDA-MEDINA; PATRICIA M. HAYWARD-JONES; OCTAVIO CARVAJAL-ZARRABAL; LUZ DEL ALBA LADRÓN DE GUEVARA-VELA; YERIKC DAVID RAMÍREZ-VILLAGÓMEZ; DULCE M. BARRADAS-DERMITZ; GEORGINA LUNA-CARRILLO; MARÍA G. AGUILAR-USCANGA

    2018-01-01

    Anthocyanins along with protocatechuic acid and quercetin have been recognized as bioactive compounds in Hibiscus sabdariffa L. aqueous extracts. Characteristic anthocyanin absorption in the visible region makes their quantification possible without the interference of the other two compounds, and also can favor its potential application as an alternative to organic-based dye sensitized solar cell, in various forms. In order to optimize measurable factors linked to the extraction of these fla...

  7. Development and evaluation of Ketoprofen sustained release matrix tablet using Hibiscus rosa-sinensis leaves mucilage

    Kaleemullah, M.; Jiyauddin, K.; Thiban, E.; Rasha, S.; Al-Dhalli, S.; Budiasih, S.; Gamal, O.E.; Fadli, A.; Eddy, Y.

    2016-01-01

    Currently, the use of natural gums and mucilage is of increasing importance in pharmaceutical formulations as valuable drug excipient. Natural plant-based materials are economic, free of side effects, biocompatible and biodegradable. Therefore, Ketoprofen matrix tablets were formulated by employing Hibiscus rosa-sinensis leaves mucilage as natural polymer and HPMC (K100M) as a synthetic polymer to sustain the drug release from matrix system. Direct compression method was used to develop susta...

  8. EFFECTS OF ALKALINE PRE-IMPREGNATION AND PULPING ON MALAYSIA CULTIVATED KENAF (HIBISCUS CANNABINUS

    Lin Suan Ang; Cheu Peng Leh; Chong Chat Lee

    2010-01-01

    This study was carried out to identify an appropriate alkaline pulping condition for Malaysia cultivated kenaf (Hibiscus cannabinus L.). The chemical composition of the kenaf bast and core fibers, and also whole stalk with different growing time were examined prior to pulping attempts. The results of various soda-AQ pulping showed that the degree of carbohydrate degradation and delignification increased with the increase of active alkali and cooking temperature, but decreased with the increas...

  9. The flower of Hibiscus trionum is both visibly and measurably iridescent.

    Vignolini, Silvia; Moyroud, Edwige; Hingant, Thomas; Banks, Hannah; Rudall, Paula J; Steiner, Ullrich; Glover, Beverley J

    2015-01-01

    Living organisms can use minute structures to manipulate the reflection of light and display colours based on interference. There has been debate in recent literature over whether the diffractive optical effects produced by epoxy replicas of petals with folded cuticles persist and induce iridescence in the original flowers when the effects of petal pigment and illumination are taken into account. We explored the optical properties of the petal of Hibiscus trionum by macro-imaging, scanning and transmission electron microscopy, and visible and ultraviolet (UV) angle-resolved spectroscopy of the petal. The flower of Hibiscus trionum is visibly iridescent, and the iridescence can be captured photographically. The iridescence derives from a diffraction grating generated by folds of the cuticle. The iridescence of the petal can be quantitatively characterized by spectrometric measurements with several square-millimetres of sample area illuminated. The flower of Hibiscus trionum has the potential to interact with its pollinators (honeybees, other bees, butterflies and flies) through iridescent signals produced by its cuticular diffraction grating. © 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.

  10. Anti-inflammatory activity and molecular mechanism of delphinidin 3-sambubioside, a Hibiscus anthocyanin.

    Sogo, Takayuki; Terahara, Norihiko; Hisanaga, Ayami; Kumamoto, Takuma; Yamashiro, Takaaki; Wu, Shusong; Sakao, Kozue; Hou, De-Xing

    2015-01-01

    Delphinidin 3-sambubioside (Dp3-Sam), a Hibiscus anthocyanin, was isolated from the dried calices of Hibiscus sabdariffa L, which has been used for folk beverages and herbal medicine although the molecular mechanisms are poorly defined. Based on the properties of Dp3-Sam and the information of inflammatory processes, we investigated the anti-inflammatory activity and molecular mechanisms in both cell and animal models in the present study. In the cell model, Dp3-Sam and Delphinidin (Dp) reduced the levels of inflammatory mediators including iNOS, NO, IL-6, MCP-1, and TNF-α induced by LPS. Cellular signaling analysis revealed that Dp3-Sam and Dp downregulated NF-κB pathway and MEK1/2-ERK1/2 signaling. In animal model, Dp3-Sam and Dp reduced the production of IL-6, MCP-1 and TNF-α and attenuated mouse paw edema induced by LPS. Our in vitro and in vivo data demonstrated that Hibiscus Dp3-Sam possessed potential anti-inflammatory properties. © 2015 International Union of Biochemistry and Molecular Biology.

  11. Analgesic activity of Hibiscus rosa sinensis Linn in rat

    Alka Sawarkar

    Full Text Available The plant Hibiscus rosa  sinensis Linn. belongs to family “Malvacecae” and Considering various medicinal properties of this plant, the leaves were collected and studied for Extractability percentage, analgesic. The herb Hibiscus rosa  sinensis  belonging to the family “Malvacecae” and is commonly known as jasvand. It is cultivated in garden throughout India native country probably china. The buds have a sweet odour and bitter taste, cooling, astringent, remove burning sensations of the body and relieve pain. In the present study aqueous, alcoholic Extracts of dried leaves of Hibiscus rosasinensis was prepared. The extractability percentage of leaves was 20%. The extract showed marked analgesic activity in a dose dependent manner. Aqueous and alcoholic extracts produced significant results at both doses (P<0.01, the findings indicated the analgesic activity of the leaves of the plant. [Vet World 2009; 2(9.000: 353-354

  12. Amino acid, mineral and fatty acid content of pumpkin seeds (Cucurbita spp) and Cyperus esculentus nuts in the Republic of Niger.

    Glew, R H; Glew, R S; Chuang, L-T; Huang, Y-S; Millson, M; Constans, D; Vanderjagt, D J

    2006-06-01

    Dried seeds and nuts are widely consumed by indigenous populations of the western Sahel, especially those who inhabit rural areas. In light of the need for quantitative information regarding the content of particular nutrients in these plant foods, we collected dried pumpkin (Cucurbita spp) seeds and nuts of Cyperus esculentus in the Republic of Niger and analyzed them for their content of essential amino acids, minerals and trace elements, and fatty acids. On a dry weight basis, pumpkin seed contained 58.8% protein and 29.8% fat. However, the lysine score of the protein was only 65% relative to the FAO/WHO protein standard. The pumpkin seed contained useful amounts of linoleic (92 microg/g dry weight) and the following elements (on a microg per g dry weight basis): potassium (5,790), magnesium (5,690), manganese (49.3), zinc (113), selenium (1.29), copper (15.4), chromium (2.84), and molybdenum (0.81), but low amounts of calcium and iron. Except for potassium (5,573 microg/g dry weight) and chromium (2.88 microg/g dry weight), the C. esculentis nuts contained much less of these same nutrients compared to pumpkin seeds. In conclusion, pumpkin seeds represent a useful source of many nutrients essential to humans. The data in this report should of practical value to public health officials in rural areas of sub-Saharan Africa.

  13. Cardioprotective effect of the Hibiscus rosa sinensis flowers in an oxidative stress model of myocardial ischemic reperfusion injury in rat

    Gauthaman, Karunakaran K; Saleem, Mohamed TS; Thanislas, Peter T; Prabhu, Vinoth V; Krishnamoorthy, Karthikeyan K; Devaraj, Niranjali S; Somasundaram, Jayaprakash S

    2006-01-01

    Background The present study investigates the cardioprotective effects of Hibiscus rosa sinensis in myocardial ischemic reperfusion injury, particularly in terms of its antioxidant effects. Methods The medicinal values of the flowers of Hibiscus rosa sinensis (Chinese rose) have been mentioned in ancient literature as useful in disorders of the heart. Dried pulverized flower of Hibiscus rosa sinensis was administered orally to Wistar albino rats (150–200 gms) in three different doses [125, 250 and 500 mg/kg in 2% carboxy methyl cellulose (CMC)], 6 days per week for 4 weeks. Thereafter, rats were sacrificed; either for the determination of baseline changes in cardiac endogenous antioxidants [superoxide dismutase, reduced glutathione and catalase] or the hearts were subjected to isoproterenol induced myocardial necrosis. Results There was significant increase in the baseline contents of thiobarbituric acid reactive substances (TBARS) [a measure of lipid per oxidation] with both doses of Hibiscus Rosa sinensis. In the 250 mg/kg treated group, there was significant increase in superoxide dismutase, reduced glutathione, and catalase levels but not in the 125 and 500 mg/kg treated groups. Significant rise in myocardial thiobarbituric acid reactive substances and loss of superoxide dismutase, catalase and reduced glutathione (suggestive of increased oxidative stress) occurred in the vehicle treated hearts subjected to in vivo myocardial ischemic reperfusion injury. Conclusion It may be concluded that flower of Hibiscus rosa sinensis (250 mg/kg) augments endogenous antioxidant compounds of rat heart and also prevents the myocardium from isoproterenol induced myocardial injury. PMID:16987414

  14. Origin and diversification of Hibiscus glaber, species endemic to the oceanic Bonin Islands, revealed by chloroplast DNA polymorphism.

    Takayama, Koji; Ohi-Toma, Tetsuo; Kudoh, Hiroshi; Kato, Hidetoshi

    2005-04-01

    Abstract Two woody Hibiscus species co-occur in the Bonin Islands of the northwestern Pacific Ocean: Hibiscus glaber Matsum. is endemic to the islands, and its putative ancestral species, Hibiscus tiliaceus L., is widely distributed in coastal areas of the tropics and subtropics. To infer isolating mechanisms that led to speciation of H. glaber and the processes that resulted in co-occurrence of the two closely related species on the Bonin Islands, we conducted molecular phylogenetic analyses on chloroplast DNA (cpDNA) sequences. Materials collected from a wide area of the Pacific and Indian Oceans were used, and two closely related species, Hibiscus hamabo Siebold Zucc. and Hibiscus macrophyllus Roxb., were also included in the analyses. The constructed tree suggested that H. glaber has been derived from H. tiliaceus, and that most of the modern Bonin populations of H. tiliaceus did not share most recent ancestry with H. glaber. Geographic isolation appears to be the most important mechanism in the speciation of H. glaber. The co-occurrence of the two species can be attributed to multiple migrations of different lineages into the islands. While a wide and overlapping geographical distribution of haplotypes was found in H. tiliaceus, localized geographical distribution of haplotypes was detected in H. glaber. It is hypothesized that a shift to inland habitats may have affected the mode of seed dispersal from ocean currents to gravity and hence resulted in geographical structuring of H. glaber haplotypes.

  15. Neuroprotective effect of the methanolic extract of Hibiscus asper leaves in 6-hydroxydopamine-lesioned rat model of Parkinson's disease.

    Hritcu, Lucian; Foyet, Harquin Simplice; Stefan, Marius; Mihasan, Marius; Asongalem, Acha Emmanuel; Kamtchouing, Pierre

    2011-09-01

    While the Hibiscus asper Hook.f. (Malvaceae) is a traditional herb largely used in tropical region of the Africa as vegetable, potent sedative, tonic and restorative, anti-inflammatory and antidepressive drug, there is very little scientific data concerning the efficacy of this. The antioxidant and antiapoptotic activities of the methanolic extract of Hibiscus asper leaves (50 and 100 mg/kg) were assessed using superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) specific activities, total glutathione (GSH) content, malondialdehyde (MDA) level (lipid peroxidation) and DNA fragmentation assays in male Wistar rats subjected to unilateral 6-hydroxydopamine (6-OHDA)-lesion. In 6-OHDA-lesioned rats, methanolic extract of Hibiscus asper leaves showed potent antioxidant and antiapoptotic activities. Chronic administration of the methanolic extract (50 and 100 mg/kg, i.p., daily, for 7 days) significantly increased antioxidant enzyme activities (SOD, GPX and CAT), total GSH content and reduced lipid peroxidation (MDA level) in rat temporal lobe homogenates, suggesting antioxidant activity. Also, DNA cleavage patterns were absent in the 6-OHDA-lesioned rats treated with methanolic extract of Hibiscus asper leaves, suggesting antiapoptotic activity. Taken together, our results suggest that the methanolic extract of Hibiscus asper leaves possesses neuroprotective activity against 6-OHDA-induced toxicity through antioxidant and antiapoptotic activities in Parkinson's disease model. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  16. Inhibition of angiotensin convertin enzyme (ACE) activity by the anthocyanins delphinidin- and cyanidin-3-O-sambubiosides from Hibiscus sabdariffa.

    Ojeda, Deyanira; Jiménez-Ferrer, Enrique; Zamilpa, Alejandro; Herrera-Arellano, Armando; Tortoriello, Jaime; Alvarez, Laura

    2010-01-08

    The beverages of Hibiscus sabdariffa calyces are widely used in Mexico as diuretic, for treating gastrointestinal disorders, liver diseases, fever, hypercholesterolemia and hypertension. Different works have demonstrated that Hibiscus sabdariffa extracts reduce blood pressure in humans, and recently, we demonstrated that this effect is due to angiotensin converting enzyme (ACE) inhibitor activity. The aim of the current study was to isolate and characterizer the constituents responsible of the ACE activity of the aqueous extract of Hibiscus sabdariffa. Bioassay-guided fractionation of the aqueous extract of dried calyces of Hibiscus sabdariffa using preparative reversed-phase HPLC, and the in vitro ACE Inhibition assay, as biological monitor model, were used for the isolation. The isolated compounds were characterized by spectroscopic methods. The anthocyanins delphinidin-3-O-sambubioside (1) and cyanidin-3-O-sambubioside (2) were isolated by bioassay-guided purification. These compounds showed IC(50) values (84.5 and 68.4 microg/mL, respectively), which are similar to those obtained by related flavonoid glycosides. Kinetic determinations suggested that these compounds inhibit the enzyme activity by competing with the substrate for the active site. The competitive ACE inhibitor activity of the anthocyanins 1 and 2 is reported for the first time. This activity is in good agreement with the folk medicinal use of Hibiscus sabdariffa calyces as antihypertensive. Copyright 2009 Elsevier Ireland Ltd. All rights reserved.

  17. 124__Mohammed_ Okra

    User

    ABSTRACT. Vegetables are rich in polyphenols, flavonoids, glucosinolates, vitamin C and their hydrolysis products which may have antioxidant and anticancer properties .This study was carried out to assess the level of total phenolic and flavonoids in selected vegetables from different sampling areas of Kano state-Nigeria.

  18. Anthocyanin extracted from Hibiscus (Hibiscus rosa sinensis L.) as a photosensitizer on nanostructured-TiO2 dye sensitized solar cells

    Ramelan, A. H.; Wahyuningsih, S.; Rosyida, N. A.; Supriyanto, E.; Saputro, S.; Hanif, Q. A.; Rinawati, L.

    2016-02-01

    Anthocyanin extracted from Hibiscus (Hibiscus rosa sinensis L) as a photosensitizer in nanostructured-TiO2 dye sensitized solar cells has been fabricated. Ultravisible visible absorption spectra of anthocyanin show an ability absorbing light in the visible region. While the nanostructed-TiO2 powder in this research was prepared by sol-gel method following annealled at a temperature of 600°C. Subsequently, the TiO2 nanostructures were characterized by XRD, XRF, and SEM. The difractogram X-ray results shown that TiO2 was built from f anatase and rutile phase. Element analysis of synthesized TiO2 by X-ray Fluorecence (XRF) shown the TiO2 content of 98,67 wt%. TiO2 layer prepared at different thickness showed the average size of cavity about 0.83 µm. These several thickness of solar cells were fabricated and were immersed into anthocyanin for 24 hours to gain sensitized TiO2 photoanode for Dye sensitised solar cells (DSSCs). These DSSCS performance were measured using I-V Keithley 2602A. The results exhibited that the sample with a TiO2 layer thickness of 4.75 ± 0.8 µm has the highest efficiency.

  19. Compostos químicos e atividade antioxidante analisados em Hibiscus rosa-sinensis L. (mimo-de-vênus) e Hibiscus syriacus L. (hibisco-da-síria)

    Silva, Analú Barbosa da; Wiest, José Maria; Carvalho, Heloisa Helena Chaves

    2016-01-01

    Resumo O Hibiscus rosa-sinensis L. e o Hibiscus syriacus L., da família Malvaceae, são utilizados na área ornamental e gastronômica internacional, mas, nos últimos anos, vêm ganhando espaço como flores comestíveis não convencionais na alimentação alternativa. Este estudo objetivou quantificar a composição centesimal, pectina e compostos fitoquímicos dessas variedades de hibisco comparados com o potencial antioxidante detectado. As amostras das flores foram coletadas em uma propriedade agroeco...

  20. Effects of Orthosiphon grandiflorus, Hibiscus sabdariffa and Phyllanthus amarus extracts on risk factors for urinary calcium oxalate stones in rats.

    Woottisin, Surachet; Hossain, Rayhan Zubair; Yachantha, Chatchai; Sriboonlue, Pote; Ogawa, Yoshihide; Saito, Seiichi

    2011-01-01

    We evaluated the antilithic effect of Orthosiphon grandiflorus, Hibiscus sabdariffa and Phyllanthus amarus extracts on known risk factors for calcium oxalate stones in rats. We divided 30 male Wistar rats into 5 equal groups. Controls were fed a standard diet and the remaining groups received a 3% glycolate diet for 4 weeks to induce hyperoxaluria. One glycolate fed group served as the untreated group and the others were given oral extracts of Orthosiphon grandiflorus, Hibiscus sabdariffa or Phyllanthus amarus at a dose of 3.5 mg daily. We collected 24-hour urine and blood samples. Kidneys were harvested for histological examination. We measured the renal tissue content of calcium and oxalate. The Hibiscus sabdariffa group showed significantly decreased serum oxalate and glycolate, and higher oxalate urinary excretion. The Phyllanthus amarus group showed significantly increased urinary citrate vs the untreated group. Histological examination revealed less CaOx crystal deposition in the kidneys of Hibiscus sabdariffa and Phyllanthus amarus treated rats than in untreated rats. Those rats also had significantly lower renal tissue calcium content than untreated rats. All parameters in the Orthosiphon grandiflorus treated group were comparable to those in the untreated group. Hibiscus sabdariffa and Phyllanthus amarus decreased calcium crystal deposition in the kidneys. The antilithic effect of Hibiscus sabdariffa may be related to decreased oxalate retention in the kidney and more excretion into urine while that of Phyllanthus amarus may depend on increased urinary citrate. In contrast, administering Orthosiphon grandiflorus had no antilithic effect. Copyright © 2011 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

  1. Mealybugs (Hemiptera: Coccomorpha: Pseudococcidae) attacking Hibiscus rosa-sinensis L. in Malaysia, with two new country records

    Sartiami, Dewi; Watson, Gillian W.; Mohamad Roff, M. N.; Idris, A. B.

    2016-11-01

    A survey of mealybugs (Hemiptera: Coccomorpha: Pseudococcidae) attacking the national flower of Malaysia, Hibiscus rosa-sisnensis L. and Hibiscus spp. (Malvaceae) was conducted in Selangor and Kuala Lumpur, Malaysia, from January to March 2016. Adult females were mounted on microscope slides in Canada balsam. The five species identified were Ferrisia dasylirii (Cockerell), Maconellicoccus hirsutus (Green), Paracoccus marginatus Williams and Granara de Willink, Phenacoccus solenopsis (Tinsley) and Pseudococcus jackbeardsleyi Gimpel & Miller. Two of these, the invasive species Ferrisia dasylirii and P. solenopsis were introduced and first recorded in Malaysia.

  2. Hibiscus sabdariffa calyx palliates insulin resistance, hyperglycemia, dyslipidemia and oxidative rout in fructose-induced metabolic syndrome rats.

    Ajiboye, Taofeek O; Raji, Hikmat O; Adeleye, Abdulwasiu O; Adigun, Nurudeen S; Giwa, Oluwayemisi B; Ojewuyi, Oluwayemisi B; Oladiji, Adenike T

    2016-03-30

    The effect of Hibiscus sabdariffa calyx extract was evaluated in high-fructose-induced metabolic syndrome rats. Insulin resistance, hyperglycemia, dyslipidemia and oxidative rout were induced in rats using high-fructose diet. High-fructose diet-fed rats were administered 100 and 200 mg kg(-1) body weight of H. sabdariffa extract for 3 weeks, starting from week 7 of high-fructose diet treatment. High-fructose diet significantly (P Hibiscus extract. Overall, aqueous extract of H. sabdariffa palliates insulin resistance, hyperglycemia, dyslipidemia and oxidative rout in high-fructose-induced metabolic syndrome rats. © 2015 Society of Chemical Industry.

  3. Responses of growth of lady’s fingers ([i]Abelmoschus esculentus [/i]L. to different treatments methods of dairy wastewater

    Rana Ibrahim Al-Dulaimi

    2014-03-01

    Full Text Available Introduction and objective. Water is one of the most important precious resources found on the earth, and are most often affected by anthropogenic activities and by industry. Pollution caused by human beings and industries is a serious concern throughout the world. Population growth, massive urbanization, rapid rate of industrialization and modern techniques in agriculture have accelerated water pollution and led to the gradual deterioration of its quality. A large quantity of waste water disposed of at sea or on land has caused environmental problems which have led to environmental pollution, economic losses and chemical risks caused by the wastewater, and its impact on agriculture. However, waste water which contain nutrients and organic matter has possible advantages for agricultural purposes. Therefore, the presented study was undertaken to assess the impact of Dairy Effluent (treated and untreated waste water on seed germination, seedling growth, dry matter production and the biochemical parameters of lady’s fingers ([i]Abelmoschus esculentus[/i] L.. Materials and methods. A field experiment in a green house was conducted to use raw and treated dairy wastewater for watering lady’s fingers (Abelmoschus esculentus L.. The plants were watered using (WW raw dairy wastewater, (T1 chemicals treatment, (T2 physical treatment, (T3 dilution method treatment and tap water (TW in pot experiments. Ten plants of each treatment /3 replicate were randomly selected and labelled for the collection of data. The data was collected sequentially, starting with chlorophyll content pre-harvest, vegetative qualities (shoot, root and seedling length and dry matter quality (shoot and root dry matter pos-tharvest. Results. The effect was seen on the germination seed and growth of the plant. The results showed inhibitory effect from dairy effluent (WW on seed germination and plant growth. Treatment with chemicals showed statistically significant differences with

  4. Is elevated CO2 in space really harmful to growth and development? A case study of Chufa (Cyperus esculentus L.) in Lunar Palace-1

    Liu, Guanghui; Dong, Chen; Fu, Yuming; Wang, Minjuan; Shao, Lingzhi; Yu, Juan; Liu, Hong

    2018-05-01

    CO2 concentration [CO2] level in artificial ecosystems such as greenhouse agriculture and space farming can easily exceed 1000 μmol mol-1 (or ppm). In order to understand how the growth and development in crop plants may respond to elevated CO2, it is necessary to determine if crop leaves in the closed artificial ecosystem have a fully developed photosynthetic apparatus, and whether or not photosynthesis in these leaves is more responsive to elevated CO2 concentration. To address this issue, we evaluated the response of photosynthetic characteristics, leaf water status and antioxidant capacity of Chufa (Cyperus esculentus L.), which is a sedge-like plant with 1-2 cm small sweet tubers in length, under elevated CO2 concentrations in an artificial closed ecosystem. The results showed that Chufa plants cultivated in the elevated CO2 environment from the seedling stage to the maturity stage were characterized by more appropriate chlorophyll content and photosynthetic rate. The photosynthetic rate of Chufa plants in the 1000 and 3000 ppm treatments was no significant difference with that in 500 ppm CO2 condition both at seedling stage and rapid growth stage. All the treatments had a high relative water content (RWC) about 60% at the maturity stage. However, there was no significant difference in membrane stability index (MSI) at the rapid growth stage. The antioxidase enzymes activities experienced a rise and a drop and reached the peak at the rapid growth stage. Elevated CO2, especially more than 1000 ppm conditions, may accelerant Chufa plants aging process.

  5. Anabolic effect of Hibiscus rosasinensis Linn. leaf extracts in immature albino male rats.

    Olagbende-Dada, S O; Ezeobika, E N; Duru, F I

    2007-01-01

    Many plants remedies have been employed in solving man's health needs especially the nutritive value which enhances health living. Aphrodisiac plants are plants with anabolic properties i.e. they help in protein synthesis and enhances sexual abilities in males. They are also known as androgenic plants because their properties are similar to that of androgen a male hormone. Cold aqueous extract of Hibiscus rosasinensis leaves is reported by local traditional practioners in Western Nigeria to be aphrodisiac. To investigate the anabolic properties of Hibiscus rosasinensis. Three groups (8/group) of immature male rats of known weights were administered equal doses of aqueous (cold and hot) and alcoholic extracts of Hibiscus rosasinensis leaves for 8 weeks. The gain in body and isolated sexual organs (testis, epididymis, seminal vesicle and prostate) weights were determined after treatment and compared to the value obtained from a fourth untreated group which served as the control. Section through the testes of both the treated and untreated rats were also examined microscopically and displayed as a photomicrograph for comparism. All data were statistically analysed and displaced in graphic form. Over the 8 weeks of treatment, the control, the cold aqueous extract dosed, hot aqueous extract dosed and alcoholic extract dosed rats gained 8%, 15%, 18% and 22% in body weights respectively. The increase in the weight of testis, epididymis, seminal vesicle and prostate of the alcoholic extract dosed rats was 19%, 30%, 31% and 40% respectively. The anabolic effect of the leaf extracts of H. rosasinensis is hereby established. More work needs to be done on these leaf extracts to know their effect on the gonadotrophin hormones which regulate the activity of the androgens in relation to spermatogenesis.

  6. Pharmacokinetics of anthocyanidin-3-glycosides following consumption of Hibiscus sabdariffa L. extract.

    Frank, Thomas; Janssen, Marlies; Netzel, Michael; Strass, Gabriele; Kler, Adolf; Kriesl, Erwin; Bitsch, Irmgard

    2005-02-01

    Pharmacokinetic parameters of several dietary anthocyanins following consumption of Hibiscus sabdariffa L. extract were determined in 6 healthy volunteers. Subjects were given a single oral dose of 150 mL of Hibiscus sabdariffa L. extract yielding 62.6 mg of cyanidin-3-sambubioside, 81.6 mg of delphindin-3-sambubioside, and 147.4 mg of total anthocyanins (calculated as cyanidin equivalents). Within 7 hours, the urinary excretion of cyanidin-3-sambubioside, delphinidin-3-sambubioside, and total anthocyanins (ie, the sum of all quantifiable anthocyanidin glycosides) was 0.016%, 0.021%, and 0.018% of the administered doses, respectively. Maximum excretion rates were determined at 1.5 to 2.0 hours after intake. The dose-normalized plasma area under the curve estimates were 0.076, 0.032, and 0.050 ng x h/mL/mg for cyanidin-3-sambubioside, delphinidin-3-sambubioside, and total anthocyanins, respectively. The dose-normalized C(max) estimates were 0.036, 0.015, and 0.023 ng/mL/mg in the same sequence. They were reached each at 1.5 hours (median) after intake. The geometric means of t1/2 were 2.18, 3.34, and 2.63 hours for cyanidin-3-sambubioside, delphinidin-3-sambubioside, and total anthocyanins, respectively. The urinary excretion of intact anthocyanins was fast and appeared to be monoexponential. To evaluate the contribution of anthocyanins to the health-protecting effects of Hibiscus sabdariffa L. extract, it will be necessary to perform further studies on both the intact glycosides and their in vivo metabolites or conjugates in human plasma and urine.

  7. THE ANTIBACTERIAL EFFECT OF ROSELLE (HIBISCUS SABDARIFFA EXTRACT AGAINST STAPHYLOCOCCUS EPIDERMIDIS IN VITRO

    Terrence Timothy Evan Lusida

    2017-04-01

    Full Text Available Infection of Staphylococcus epidermidis is still a common problem in many hospitals. Factor determining biofilm formation makes it harder for atibiotics to cure the infection. Roselle (Hibiscus sabdariffa, a well known traditional medicine plant, is a potential candidate as a drug againts infectious disease. The purpose of this research is to investigate the antibacterial effect of ethanol extract from Roselle (Hibiscus sabdariffa calyx againts the growth of Staphylococcus epidermidis. Assessment for antibacterial effect is performed using broth diffusion method. The extract is made by maceration of the calyx of Roselle in 96% ethanol. Extracts with concentration of 125, 62.5, 31.25, 15.63, 7.81, 3.90, 1.95, 0.97, 0.48, 0.24 mg/mL are added into separated Mueller-Hinton broths (MHB, which have already been inoculated by Staphylococcus epidermidis. As for bacterial growth control, we used MHB with bacterial inoculation, while sterility control we used mixture of extract and MHB. Then from each broth, the solutions are added into separated nutrition agar plates. Replications are done three times. Clarity and bacterial growth are observed after 24 hours of incubation. However, clarity cannot be observed in 36 broth, but bacterial growth is observed on the plate for concentration 0.97, 0.48, and 0.24 mg/mL. Therefore Minimum inhibitory concentration (MIC cannot be determined because the extract’s color interfere the observation. While minimum bactericidal concentration (MBC, the last concentration before the concentration where the bacteria are still viable, is 1.95 mg/mL. Based on the result of the research, the Roselle calyx ethanol extract (Hibiscus sabdariffa through dilution method with a concentration of 1.95 mg / mL can kill Staphylococcus epidermidis and in order to find MIC in collored and turbid solution (before being incubated in incubator, we can consider using agar dilution methode or microdilution methode.

  8. Aplicação da técnica de dispersão da matriz em fase sólida (DMFS na análise de pesticidas em quiabo por CG-EM Application of the matrix solid phase dispersion (MSPD technique in the analysis of pesticides in okra by GC-MS

    Haroldo Silveira Dórea

    2004-12-01

    Full Text Available A matrix solid phase dispersion and gas chromatography-mass selective detection method for the simultaneous determination of monocrotophos, methyl parathion, cypermethrin and deltamethrin in okra is described. Analyses of 2 g of fortified okra (0.05-0.75 mg kg-1 showed an average recovery of 96.2% (71.4-128.4% and average relative standard deviation of 11.7% (1.4-37.1%. The cypermethrin recovery at the lower level was above 130%. The limit of detection ranged from 0.02 to 0.15 mg kg-1. The procedure was applied to the okra samples and has found 0.56 mg kg-1 of cypermethrin-cis, 0.75 mg kg-1 of cypermethrin-trans and 2.71 mg kg-1 of deltamethrin.

  9. Extraction of lipid components from hibiscus seeds by supercritical carbon dioxide and ethanol mixtures

    Holser, Ronald A.; King, J. W. (Jerry W.); Bost, G.

    2002-01-01

    The genus Hibiscus exhibits great diversity in the production of natural materials with edible and industrial applications. The seeds of twelve varieties of Hibiscus were investigated as a source for triglycerides and phospholipids that could be used in functional foods. Lipid components were extracted from seed samples ground to a nominal particle diameter of 0.1 mm. Extractions were performed with an ISCO model 3560 supercritical fluid extractor using carbon dioxide and a mixture of carbon dioxide modified with ethanol. The neutral lipids were extracted with carbon dioxide at 80 C and 5370 MPa for 45 min. Polar lipids were subsequently extracted with a mixture of carbon dioxide and 15% ethanol at the same temperature and pressure. High performance liquid chromatography (HPLC) was used to analyze extracts for major neutral and polar lipid classes. A silica column was used with a solvent gradient of hexane/isopropanol/ water and ultraviolet (UV) and evaporative light scattering detectors (ELSD). An aliquot of each triglyceride fraction was trans-methylated with sodium methoxide and analyzed by gas chromatography to obtain the corresponding fatty acid methyl esters. The total lipids extracted ranged from 8.5% for a variety indigenous to Madagascar (H. calyphyllus) to 20% for a hybrid species (Georgia Rose). The average oil yield was 11.4% for the other varieties tested. The fatty acid methyl ester analysis displayed a high degree of unsaturation for all varieties tested, e. g., 75 ' 83%. Oleic, linoleic, and linolenic fatty acids were the predominate unsaturated fatty acids with only minor amounts of C14, C18, and C20 saturated fatty acids measured. Palmitic acid was identified as the predominate saturated fatty acid. The distribution of the major phospholipids, i. e., phosphatidylethanolamine, phosphatidic acid, phosphatidylserine, phosphatidylcholine, and lysophosphatidylcholine, was found to vary significantly among the hibiscus species examined

  10. Mucilage-secreting structures of Hibiscus pernambucensis Arruda (Malvaceae): distribution, morphoanatomical and histochemical characterization

    Rocha, Joecildo Francisco; Pimentel, Rafael Ribeiro; Machado, Silvia Rodrigues [UNESP

    2011-01-01

    No presente trabalho foram estudadas as estruturas secretoras de mucilagem de Hibiscus pernambucensis aos microscópios de luz e de varredura. Conhecida como guaxima-do-mangue e algodão-do-brejo, é espécie nativa encontrada no litoral brasileiro, vegetando áreas de manguezal e restinga, tendo grande importância ecológica nestes ecossistemas. É importante produtora de fibras têxteis e celulose, fornece abundante mucilagem suscetível das mesmas aplicações medicinais de outras malváceas, além da ...

  11. Usage of immobilized porcine pancreas lipase in the hydrolysis of roselle (Hibiscus sabdariffa L.) seed oil

    Ai, Chau Tran Diem; Linh, Vo Thi Hong; Yen, Tran Thi Ngoc; Nguyen, Nguyen Thi; Hoa, Phan Ngoc

    2017-09-01

    This study focused on the comparison among the usage of immobilized porcine pancreas lipase (PPL) on different hydrotalcite carriers (uncalcined and calcined hydrotalcite - like compound Mg /Al) and free lipase as the catalysts to hydrolyze of roselle (Hibiscus sabdariffa L.) seed oil. The reaction conditions were investigated including the ratio of oil to buffer, ratio of enzyme to substrate, the temperature of the hydrolysis, pH. The calcined hydrotalcite showed a higher lipase immobilization yield and a better reusability than the uncalcined hydrotalcite (87.15% and 86.78%, respectively).

  12. Phytochemical, sensory attributes and aroma stability of dense phase carbon dioxide processed Hibiscus sabdariffa beverage during storage.

    Ramírez-Rodrigues, Milena M; Plaza, Maria L; Azeredo, Alberto; Balaban, Murat O; Marshall, Maurice R

    2012-10-01

    The effect of dense phase carbon dioxide (DPCD) processing (34.5 MPa, 8% CO₂, 6.5 min, and 40 °C) on phytochemical, sensory and aroma compounds of hibiscus beverage was compared to a conventional thermal process (HTST) (75 °C for 15 s) and a control (untreated beverage) during refrigerated storage (4 °C). The overall likeability of the hibiscus beverage for all treatments was not affected by storage up to week 5. DPCD process retained more aroma volatiles as compared to HTST. Aroma profiles in the beverages were mainly composed of alcohols and aldehydes with 1-octen-3-ol, decanal, octanal, 1-hexanol, and nonanal as the compounds with the highest relative percentage peak areas. A loss of only 9% anthocyanins was observed for the DPCD processed hibiscus beverage. Phytochemical profiles in the hibiscus beverage included caffeoylquinic acids, anthocyanins, and flavonols. No major changes in total phenolics and antioxidant capacity occurred during the 14 weeks of storage. Copyright © 2012 Elsevier Ltd. All rights reserved.

  13. Complete nucleotide sequence of a novel Hibiscus-infecting Cilevirus from Florida and its relationship with closely associated Cileviruses

    The complete nucleotide sequence of a recently discovered Florida (FL) isolate of Hibiscus infecting Cilevirus (HiCV) was determined by Sanger sequencing. The movement- and coat- protein gene sequences of the HiCV-FL isolate are more divergent than other genes of the previously sequenced HiCV-HA (Ha...

  14. Impact of kenaf (Hibiscus cannabinus L.) leaf, bark, and core extracts on germination of five plant species

    The chemical interaction between plants, which is referred to as allelopathy, may result in the inhibition of plant growth and development. The objective of this research was to determine the impact of kenaf (Hibiscus cannabinus L.) plant extracts on the germination and post-germination development ...

  15. Genetic variability for phenotype, seed production, oil content, and fatty acid composition among 17 Roselle (Hibiscus sabdariffa) accessions

    Seed oil and fatty acids in plants have human health implications. Oil from roselle (Hibiscus sabdariffa L.) seeds are used in Taiwan as a diuretic, laxative, and tonic. The objectives of this study were to evaluate seeds from 17 roselle accessions for oil and fatty acid variation in a greenhouse. S...

  16. The Role of Leaves in Photocontrol of Flower Bud Abscission in Hibiscus rosa-sinensis L. 'Nairobi'

    Meeteren, van U.; Gelder, van A.

    2000-01-01

    When compared with exposure to darkness, exposing Hibiscus rosa-sinensis L. 'Nairobi' plants to red light (635 to 685 nm, 2.9 μmol?m-2?s-1) delayed flower bud abscission, while exposure to far-red light (705 to 755 nm, μmol?m-2?s-1) accelerated this process. Flower bud abscission in response to

  17. Growth and biomass productivity of kenaf (Hibiscus cannabinus, L.) under different agricultural inputs and management practices in central Greece

    Danalatos, N.G.; Archontoulis, S.V.

    2010-01-01

    The growth and biomass productivity of kenaf (Hibiscus cannabinus, L.) cultivars Tainung 2 and Everglades 41 were determined under three irrigation applications (low: 25%, moderate: 50% and fully: 100% of maximum evapotranspiration; ETm), four nitrogen dressings (0, 50, 100 and 150 kg hat), two

  18. Multi-locus DNA barcoding identifies matK as a suitable marker for species identification in Hibiscus L.

    Poovitha, Sundar; Stalin, Nithaniyal; Balaji, Raju; Parani, Madasamy

    2016-12-01

    The genus Hibiscus L. includes several taxa of medicinal value and species used for the extraction of natural dyes. These applications require the use of authentic plant materials. DNA barcoding is a molecular method for species identification, which helps in reliable authentication by using one or more DNA barcode marker. In this study, we have collected 44 accessions, representing 16 species of Hibiscus, distributed in the southern peninsular India, to evaluate the discriminatory power of the two core barcodes rbcLa and matK together with the suggested additional regions trnH-psbA and ITS2. No intraspecies divergence was observed among the accessions studied. Interspecies divergence was 0%-9.6% with individual markers, which increased to 0%-12.5% and 0.8%-20.3% when using two- and three-marker combinations, respectively. Differentiation of all the species of Hibiscus was possible with the matK DNA barcode marker. Also, in two-marker combinations, only those combinations with matK differentiated all the species. Though all the three-marker combinations showed 100% species differentiation, species resolution was consistently better when the matK marker formed part of the combination. These results clearly showed that matK is more suitable when compared to rbcLa, trnH-psbA, and ITS2 for species identification in Hibiscus.

  19. Investigation of the bacterial retting community of kenaf (Hibiscus cannabinus) under different conditions using next-generation semiconductor sequencing

    The use of the natural fibers requires the development of cost-efficient processing of fibers with consistent, uniform properties. The microbial communities associated with kenaf (Hibiscus cannabinus) plant fibers during retting were determined in an effort to identify possible means of accelerating...

  20. Molecular cloning, characterization and expression of the caffeic acid O-methyltransferase (COMT) ortholog from kenaf (Hibiscus cannabinus)

    We cloned the full-length of the gene putatively encoding caffeic acid O-methyltransferase (COMT) from kenaf (Hibiscus cannabinus L.) using degenerate primers and the RACE (rapid amplification of cDNA ends) method. Kenaf is an herbaceous and rapidly growing dicotyledonous plant with great potential ...

  1. Characterization of developmental and stress mediated expression of cinnamoyl-CoA reductase (CCR) in kenaf (Hibiscus cannabinus L.)

    Cinnamoyl-CoA reductase (CCR) is an important enzyme for lignin biosynthesis as it catalyzes the first specific committed step in monolignol biosynthesis. We have cloned a full length coding sequence of CCR from kenaf (Hibiscus cannabinus L.), which contains a 1,020-bp open reading frame (ORF), enco...

  2. Field evaluation of two systemic neonicotinoid insecticides against pink hibiscus mealybug (Maconellicoccus hirsutus (Green))on mulberry trees

    Infestations of the pink hibiscus mealybug, Maconellicoccus hirsutus (Green), in ornamental trees were already in an advanced state at the time of its discovery in the Imperial Valley of California (USA) in August 1999. Concern about the spread of M. hirsutus beyond the Imperial Valley led to the p...

  3. Wilt, crown, and root rot of common rose mallow (Hibiscus moscheutos) caused by a novel Fusarium sp

    A new crown and root rot disease of landscape plantings of the malvaceous ornamental common rose mallow (Hibiscus moscheutos) was first detected in Washington State in 2012. The main objectives of this study were to complete Koch's postulates, document the disease sypmtoms photographically, and iden...

  4. In Vitro Digestibility of Aluminum from Hibiscus sabdariffa Hot Watery Infusion and Its Concentration in Urine of Healthy Individuals

    Frankova, A.; Malik, J.; Drabek, O.; Szakova, J.; Sperlingova, I.; Kloucek, P.; Novy, P.; Tejnecky, V.; Landa, Přemysl; Leuner, O.; Kokoska, L.

    2016-01-01

    Roč. 174, č. 2 (2016), s. 267-273 ISSN 0163-4984 Institutional support: RVO:61389030 Keywords : dialysis dementia * tea * bioavailability * speciation * toxicity * Aluminum * In vitro digestion * Hot watery infusion * Urine * Hibiscus sabdariffa L Subject RIV: EF - Botanics Impact factor: 2.399, year: 2016

  5. Components in aqueous Hibiscus rosa-sinensis flower extract inhibit in vitro melanoma cell growth

    Karina H. Goldberg

    2017-01-01

    Full Text Available Skin cancer is extremely common, and melanoma causes about 80% of skin cancer deaths. In fact, melanoma kills over 50 thousand people around the world each year, and these numbers are rising. Clearly, standard treatments are not effectively treating melanoma, and alternative therapies are needed to address this problem. Hibiscus tea has been noted to have medicinal properties, including anticancer effects. Extracts from Hibiscus have been shown to inhibit the growth of a variety of cancer cells. In particular, recent studies found that polyphenols extracted from Hibiscus sabdariffa by organic solvents can inhibit melanoma cell growth. However, effects of aqueous extracts from Hibiscus rosa-sinesis flowers, which are commonly used to make traditional medicinal beverages, have not been examined on melanoma cells. Here, we report that aqueous H. rosa-sinesis flower extract contains compounds that inhibit melanoma cell growth in a dose dependent manner at concentrations that did not affect the growth of nontransformed cells. In addition, these extracts contain low molecular weight growth inhibitory compounds below 3 kD in size that combine with larger compounds to more effectively inhibit melanoma cell growth. Future work should identify these compounds, and evaluate their potential to prevent and treat melanoma and other cancers.

  6. Kenaf as a potential source of pulp in Australia. Proceedings of Kenaf Conference, May 28-29, 1981, Brisbane, Queensland. [Hibiscus Cannabinus

    Wood, I.M.; Stewart, G.A. (eds.)

    1981-01-01

    Papers by different authors are presented in 8 sections on kenaf (Hibiscus cannabinus), a possible substitute for wood pulp. Agronomy and physiology; Selection and breeding; Pests and diseases; By-products; Harvesting; Farming systems; Pulping techniques; and Economics and marketing.

  7. In vitro antioxidant, anti-inflammatory, cytotoxic activities against prostate cancer of extracts from Hibiscus sabdariffa leaves.

    Worawattananutai, Patsorn; Itharat, Arunporn; Ruangnoo, Srisopa

    2014-08-01

    Hibiscus sabdariffa (HS) leaves are a vegetable, which is used as a healthy sour soup for protection against chronic diseases in Thai traditional medicine. To investigate antioxidant, anti-inflammatory and cytotoxic activities of Hibiscus sabdariffa leave extracts from diferent extraction methods. Fresh and dry Hibiscus sabdariffa leaves were extracted by various methods such as maceration with 95% and 50% ethanol, squeeze, and boiling with water or decoction. All extracts were testedfor antioxidant activity by using DPPH radical scavenging assay, anti-inflammatory activity by determination on inhibitory effect of nitric oxide production on RAW264. 7 cell. Cytotoxic activity also tested against human prostate cancer cell line (PC-3) by using sulforhodamine B (SRB) assay. Total phenolic content determined by the Folin-Ciocalteu colorimetric method. The results found that the 95% ethanolic extract of Hibiscus sabdariffa dried leaves (HSDE95) showed the highest antioxidant activity with an EC50 of 34.51±2.62 μg/ml and had the highest phenolic content (57.00±3.73 mg GAE/g). HSDE95 also showed potent cytotoxicity against prostate cancer cell line with an IC50 of 8.58±0.68 μg/ml whereas HSDE95 and all of extracts ofHibiscus sabdariffa leaves had no anti-inflammatory activity. The obtained results revealed that HSDE95 extract showedpotent cytotoxic activity against prostate cancer cells but low antioxidant and anti-inflammatory activities. This extract should be further isolated as active compounds against prostate cancer.

  8. In vitro inhibitory activities of the extract of Hibiscus sabdariffa L. (family Malvaceae) on selected cytochrome P450 isoforms.

    Johnson, Showande Segun; Oyelola, Fakeye Titilayo; Ari, Tolonen; Juho, Hokkanen

    2013-01-01

    Literature is scanty on the interaction potential of Hibiscus sabdariffa L., plant extract with other drugs and the affected targets. This study was conducted to investigate the cytochrome P450 (CYP) isoforms that are inhibited by the extract of Hibiscus sabdariffa L. in vitro. The inhibition towards the major drug metabolizing CYP isoforms by the plant extract were estimated in human liver microsomal incubations, by monitoring the CYP-specific model reactions through previously validated N-in-one assay method. The ethanolic extract of Hibiscus sabdariffa showed inhibitory activities against nine selected CYP isoforms: CYP1A2, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1 and CYP3A4. The concentrations of the extract which produced 50% inhibition of the CYP isoforms ranged from 306 µg/ml to 1660 µg/ml, and the degree of inhibition based on the IC50 values for each CYP isoform was in the following order: CYP1A2 > CYP2C8 > CYP2D6 > CYP2B6 > CYP2E1 > CYP2C19 > CYP3A4 > CYP2C9 > CYP2A6. Ethanolic extract of Hibiscus sabdariffa caused inhibition of CYP isoforms in vitro. These observed inhibitions may not cause clinically significant herb-drug interactions; however, caution may need to be taken in co-administering the water extract of Hibiscus sabdariffa with other drugs until clinical studies are available to further clarify these findings.

  9. [Chemical Constituents from Leaves of Hibiscus syriacus and Their α-Glucosidase Inhibitory Activities].

    Wei, Qiang; Ji, Xiao-ying; Xu, Fei; Li, Qian-rong; Yin, Hao

    2015-05-01

    To study the chemical constituents from Hibiscus syriacus leaves and their α-glucosidase inhibitory activities. Column chromatography including macroporous resins, silica gel and Sephadex LH-20 were used for the isolation and purification of all compounds. Spectroscopic methods including physical and chemical properties, 1H-NMR and 13C-NMR were used for the identification of structures. Their α-glucosidase inhibitory activities were detected by a 96-well microplate. 15 compounds were isolated and identified as β-sitosterol(1), β-daucostero (2), β-amyrin (3), oleanolic acid (4), stigmast-4-en-3-one (5), friedelin (6), syriacusin A (7), kaempferol (8), isovitexin (9), vitexin (10), apigenin (11), apigenin-7-O-β-D-glucopyranoside (12), luteolin-7-O-β-D-glucopyranoside (13), vitexin-7-O-β-D-glucopyranoside (14) and rutin (15). All the compounds are isolated from the leaves of Hibiscus syriacus for the first time. Taking acarbose as positive control, the α-glucosidase inhibitory activities of 15 compounds were evaluated. Compounds 7 and 9 have shown strong α-glucosidase inhibitory activities with IC50 of 39.03 ± 0.38 and 32.12 ± 0.62 mg/L, inhibition ratio of 94.95% and 97.15%, respectively.

  10. OPTIMISATION OF HIBISCUS SABDARIFFA AS A NATURAL COAGULANT TO TREAT CONGO RED IN WASTEWATER

    MUN Y. YONG

    2016-02-01

    Full Text Available The process of coagulation is commonly practiced in water and wastewater treatment to reduce level of dissolved chemical, turbidity and so on with the usage of coagulant. Aluminium sulphate (alum is the most commonly used coagulant, however, recent studies show that residual aluminium in drinking water and sludge may induce Alzheimer’s disease and environmental issues. Natural coagulant which is environmental friendly and non-toxic is developed as an alternative to overcome these issues. In this work, Hibiscus Sabdariffa was studied as natural coagulant to treat dye wastewater containing Congo red. The seeds were extracted with different solvent such as distilled water, 0.5 M NaCl and 0.05 M NaOH to extract the coagulation agent. The working parameters were optimised using Response Surface Methodology (RSM. 0.5 M NaCl was found to have highest colour removal of 95.1 % among the solvents. In addition, Hibiscus Sabdariffa seed was found to be an effective coagulant that has 91.2 % colour removal at the optimal working condition of pH 2, 190 mg/L coagulant dosage at 400 ppm of dye concentration. It was also been identified that the performance of natural coagulant is comparable with conventional coagulant, aluminium sulphate with colour removal of 91.2 % and 92.3 % respectively.

  11. Hibiscus sabdariffa (Roselle) Extracts and Wine: Phytochemical Profile, Physicochemical Properties, and Carbohydrase Inhibition.

    Ifie, Idolo; Marshall, Lisa J; Ho, Peter; Williamson, Gary

    2016-06-22

    Three varieties of Hibiscus sabdariffa were analyzed for their phytochemical content and inhibitory potential on carbohydrate-digesting enzymes as a basis for selecting a variety for wine production. The dark red variety was chosen as it was highest in phenolic content and an aqueous extract partially inhibited α-glucosidase (maltase), with delphinidin 3-O-sambubioside, cyanidin 3-O-sambubioside, and 3-O-caffeoylquinic acid accounting for 65% of this activity. None of the varieties significantly inhibited α-amylase. Regarding Hibiscus sabdariffa wine, the effect of fermentation temperature (20 and 30 °C) on the physicochemical, phytochemical, and aroma composition was monitored over 40 days. The main change in phytochemical composition observed was the hydrolysis of 3-O-caffeolquinic acid and the concomitant increase of caffeic acid irrespective of fermentation temperature. Wine fermented at 20 °C was slightly more active for α-glucosidase inhibition with more fruity aromas (ethyl octanoate), but there were more flowery notes (2-phenylethanol) at 30 °C.

  12. Anti-Escherichia coli effect of Hibiscus sabdariffa L. in a meat model

    Marcelo Pinto PAIM

    Full Text Available Abstract Hibiscus sabdariffa L. is used in traditional medicine because of its bioactive properties, such as antioxidant and antibacterial. Escherichia coli is a Gram-negative bacteria and as an indicator of contamination in food. The aim of this work was to evaluate the anti-Escherichia coli effect and the change in pH on the control of aerobic mesophilic microorganisms, using hydroethanolic extract of H. sabdariffa L. in different concentrations in a meat model, verifying its potential as food additive for microbiological stability on ground beef during cooling storage. For the preparation of the treatments, the meat experimental units were elaborated with different concentrations of the vegetal extract (5, 10, 15 and 20%, ground beef and contaminated with E. coli. For pH evaluation, the meat experimental units were added different percentages of hydroethanolic extract. The H. sabdariffa L. antibacterial action reduced two logarithmic levels in practically all treatments. The best pH result was obtained in the meat containing 30% of the extract. The hydroethanolic extracts of Hibiscus sabdariffa L. showed anti-Escherichia coli activity in the presence of refrigerated ground beef. Analyzing the pH results and the count of aerobic mesophilic bacteria, it is possible this extract to be used as a natural food additive.

  13. Polyphenolic extract from Hibiscus sabdariffa reduces body fat by inhibiting hepatic lipogenesis and preadipocyte adipogenesis.

    Kao, Erl-Shyh; Yang, Mon-Yuan; Hung, Chia-Hung; Huang, Chien-Ning; Wang, Chau-Jong

    2016-01-01

    Diets high in fat lead to excess lipid accumulation in adipose tissue, which is a crucial factor in the development of obesity, hepatitis, and hyperlipidemia. In this study, we investigated the anti-obesity effect of Hibiscus sabdariffa extract (HSE) in vivo. Hamsters fed a high-fat diet (HFD) develop symptoms of obesity, which were determined based on body weight changes and changes in plasma and serum triglycerides, free fatty acid concentrations, total cholesterol levels, LDL-C levels, HDL-C levels, and adipocyte tissue weight. HFD-fed hamsters were used to investigate the effects of HSE on symptoms of obesity such as adipogenesis and fatty liver, loss of blood glucose regulation, and serum ion imbalance. Interestingly, HSE treatment effectively reduced the effects of the HFD in hamsters in a dose-dependent manner. Further, after inducing maturation of preadipocytes, Hibiscus sabdariffa polyphenolic extract (HPE) was shown to suppress the adipogenesis of adipocytes. However, HPE does not affect the viability of preadipocytes. Therefore, both HSE and HPE are effective and viable treatment strategies for preventing the development and treating the symptoms of obesity.

  14. Laboratory Evaluation of Different Insecticides against Hibiscus Mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae

    Samman Fatima

    2016-01-01

    Full Text Available Hibiscus mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae, is the major pest of many vegetables, fruits, crops, and ornamental plants causing losses to the farmers and its control has been an issue of significance in the pest management. This study was aimed at evaluating different concentrations (0.06%, 0.1%, and 0.14% of Telsta, Advantage, Talstar, Imidacloprid, and their mixtures against hibiscus mealybug in the Laboratory of Systematics and Pest Management at University of Gujrat, Pakistan. The toxic effect was evaluated in the laboratory bioassay after 24 and 48 h of the application of insecticides. The highest mortality (95.83% was shown by Talstar and Talstar + Imidacloprid at the concentration of 0.14% after 48 h followed by Advantage + Talstar with 87.50% mortality at 0.14% concentration after 48 h of application. The study also showed that the least effective treatment observed was Advantage + Telsta with no mortality after 24 h and 25% mortality after 48 h at 0.14% concentration. The study revealed that the concentration 0.14% was highly effective in lowering the mealybug population and insecticide mixtures were effective in reducing mealybug density. The study emphasizes the use of such insecticide mixtures to develop better management strategy for mealybug populations attacking ornamental plants. However effects of such insecticide mixtures on other organisms and biological control agents should be checked under field conditions.

  15. A distributional and cytological survey of the presently recognized taxa of Hibiscus section Furcaria (Malvaceae.

    F. Douglas Wilson

    2006-01-01

    Full Text Available Hibiscus section Furcaria is a natural group of plants that presently includes 109 recognized taxa. Taxa are found in subsaharan Africa, India, southeastern Asia, Malesia, Australia, islands of the Pacific basin, the Caribbean, North, Central, and South America. The basic chromosome number is x = 18. In nature, ploidy levels range from diploid to decaploid. The taxa exhibit a remarkable amount of genome diversity. At least 13 genomes have been identified, some distributed widely and others with more restricted distributions. No modern taxonomic monograph ofHibiscus section Furcaria exists, but a number of regional studies have appeared that are essentially global in extent. Also, a number of studies of chromosome numbers and genome relationships have been published. The present paper includes a census of all the presently accepted taxa, the geographical distribution of each taxon, and chromosome numbers and genome designations of the 49 taxa for which the information is available. Important mechanisms of speciation include genome divergence at the diploid level, followed by hybridization and allopolyploidy, significant species radiation at the tetraploid and hexaploid levels, and the development of even higher levels of allopolyploids.

  16. Antidepressant-like effects of methanol extract of Hibiscus tiliaceus flowers in mice

    2012-01-01

    Background Hibiscus tiliaceus L. (Malvaceae) is used in postpartum disorders. Our purpose was to examine the antidepressant, anxiolytic and sedative actions of the methanol extract of H. tiliaceus flowers using animal models. Methods Adult male Swiss albino mice were treated with saline, standard drugs or methanol extract of H. tiliaceus and then subjected to behavioral tests. The forced swimming and tail suspension tests were used as predictive animal models of antidepressant activity, where the time of immobility was considered. The animals were submitted to the elevated plus-maze and ketamine-induced sleeping time to assess anxiolytic and sedative activities, respectively. Results Methanol extract of H. tiliaceus significantly decreased the duration of immobility in both animal models of antidepressant activity, forced swimming and tail suspension tests. This extract did not potentiate the effect of ketamine-induced hypnosis, as determined by the time to onset and duration of sleeping time. Conclusion Our results indicate an antidepressant-like profile of action for the extract of Hibiscus tiliaceus without sedative side effect. PMID:22494845

  17. Effect of Chronic Gamma Irradiation on Kenaf (Hibiscus cannabinus. L) Variety V36

    Mohd Zulmadi Sani; Faiz Ahmad; Mustapha Akil; Zaiton Ahmad; Affrida Abu Hassan; Abdul Rahim Harun

    2016-01-01

    Kenaf (Hibiscus cannabinus) is originated from Africa and used in making various products such as animal food pallet, clothes, particle board, bio-plastics, paper and many other fibre related products. Due to its commercial value in various industries, research has been carried out to obtain kenaf with improved traits. Mutation breeding using ionizing radiation has been used widely and proven to be effective as an alternative method in plant improvement. In this study, chronic gamma irradiation on kenaf (Hibiscus cannabinus. L) Variety V36 was carried out at Gamma Greenhouse (GGH) facility at Malaysia Nuclear Agency. Kenaf seeds were planted in pots at the shade house and transferred to GGH after 7 days. These seedlings were subjected to various dose rates of 0.3 Gy/ hour, 0.17 Gy/ hour, 0.11 Gy /hour, 0.05 Gy/ hour, 0.03 Gy/ hour, 0.02 Gy/ hour, and 0 Gy/ hour (control). Plant survival and morphological data such as plant height and stem girth, were observed. From the result obtain, at accumulated dose of 230 Gy (Ring 4), plant showed the highest average plant height, stem girth and highest morphological changes. The finding of this study is useful in determining the most suitable dose rate for chronic gamma irradiation in the future. (author)

  18. [Therapeutic potential of Hibiscus sabdariffa: a review of the scientific evidence].

    Guardiola, Soledad; Mach, Núria

    2014-05-01

    Infusion of Hibiscus sabdariffa (H. sabdariffa) is a very popular drink in many parts of the world. Its phytochemical composition is associated to antioxidant, hypotensive, and antiatherosclerotic effects. However, the molecular mechanisms involved in these processes are not well known. The aim of this review was to report the scientific evidence supporting that regular use of H. sabdariffa decreases oxidative stress, atherosclerosis, lipid profile, and blood pressure. A search of recent publications was made in the following specialized electronic databases: Elsevier Journal, SciELO, FSTA, Science Direct, Springer Link, and NCBI. Results of research conducted in clinical trials in humans and in animal models and cell cultures were recorded. Keywords used included Hibiscus sabdariffa, oxidative stress, polyphenols, hypertension, atherosclerosis, and lipid profile. Results of the different articles suggested a possible therapeutic effect of H. sabdariffa extracts on oxidative stress, lipid profile, hypertension, and atherosclerosis thanks to its composition rich in phenolic compounds. Anthocyanins significantly decrease LDL oxidation, inhibit adipogenesis by regulating adipogenic signaling pathways and transcription factors, and modulate gene expression of certain microRNAs. No adverse events or side effects were reported. Further more homogeneous, placebo-controlled studies in humans are needed to state that H. sabdariffa has therapeutic efficacy in humans. Copyright © 2013 SEEN. Published by Elsevier Espana. All rights reserved.

  19. Determination of suitable microspore stage and callus induction from anthers of kenaf (Hibiscus cannabinus L.).

    Ibrahim, Ahmed Mahmood; Kayat, Fatimah Binti; Hussin, Zeti Ermiena Surya Mat; Susanto, Dwi; Ariffulah, Mohammed

    2014-01-01

    Kenaf (Hibiscus cannabinus L.) is one of the important species of Hibiscus cultivated for fiber. Availability of homozygous parent lines is prerequisite to the use of the heterosis effect reproducible in hybrid breeding. The production of haploid plants by anther culture followed by chromosome doubling can be achieved in short period compared with inbred lines by conventional method that requires self pollination of parent material. In this research, the effects of the microspore developmental stage, time of flower collection, various pretreatments, different combinations of hormones, and culture condition on anther culture of KB6 variety of Kenaf were studied. Young flower buds with immature anthers at the appropriate stage of microspore development were sterilized and the anthers were carefully dissected from the flower buds and subjected to various pretreatments and different combinations of hormones like NAA, 2,4-D, Kinetin, BAP, and TDZ to induce callus. The best microspore development stage of the flower buds was about 6-8 mm long collected 1-2 weeks after flower initiation. At that stage, the microspores were at the uninucleate stage which was suitable for culture. The best callus induction frequency was 90% in the optimized semisolid MS medium fortified with 3.0 mg/L BAP + 3.0 mg/L NAA.

  20. Antidepressant-like effects of methanol extract of Hibiscus tiliaceus flowers in mice

    Vanzella Cláudia

    2012-04-01

    Full Text Available Abstract Background Hibiscus tiliaceus L. (Malvaceae is used in postpartum disorders. Our purpose was to examine the antidepressant, anxiolytic and sedative actions of the methanol extract of H. tiliaceus flowers using animal models. Methods Adult male Swiss albino mice were treated with saline, standard drugs or methanol extract of H. tiliaceus and then subjected to behavioral tests. The forced swimming and tail suspension tests were used as predictive animal models of antidepressant activity, where the time of immobility was considered. The animals were submitted to the elevated plus-maze and ketamine-induced sleeping time to assess anxiolytic and sedative activities, respectively. Results Methanol extract of H. tiliaceus significantly decreased the duration of immobility in both animal models of antidepressant activity, forced swimming and tail suspension tests. This extract did not potentiate the effect of ketamine-induced hypnosis, as determined by the time to onset and duration of sleeping time. Conclusion Our results indicate an antidepressant-like profile of action for the extract of Hibiscus tiliaceus without sedative side effect.

  1. Hibiscus sabdariffa leaf polyphenolic extract induces human melanoma cell death, apoptosis, and autophagy.

    Chiu, Chun-Tang; Hsuan, Shu-Wen; Lin, Hui-Hsuan; Hsu, Cheng-Chin; Chou, Fen-Pi; Chen, Jing-Hsien

    2015-03-01

    Melanoma is the least common but most fatal form of skin cancer. Previous studies have indicated that an aqueous extract of Hibiscus sabdariffa leaves possess hypoglycemic, hypolipidemic, and antioxidant effects. In this study, we want to investigate the anticancer activity of Hibiscus leaf polyphenolic (HLP) extract in melanoma cells. First, HLP was exhibited to be rich in epicatechin gallate (ECG) and other polyphenols. Apoptotic and autophagic activities of HLP and ECG were further evaluated by DAPI stain, cell-cycle analysis, and acidic vascular organelle (AVO) stain. Our results revealed that both HLP and ECG induced the caspases cleavages, Bcl-2 family proteins regulation, and Fas/FasL activation in A375 cells. In addition, we also revealed that the cells presented AVO-positive after HLP treatments. HLP could increase the expressions of autophagy-related proteins autophagy-related gene 5 (ATG5), Beclin1, and light chain 3-II (LC3-II), and induce autophagic cell death in A375 cells. These data indicated that the anticancer effect of HLP, partly contributed by ECG, in A375 cells. HLP potentially could be developed as an antimelanoma agent. © 2015 Institute of Food Technologists®

  2. INHIBITION OF HUMAN LOW-DENSITY LIPOPROTEINS OXIDATION BY Hibiscus radiatus CUV. CALYCES EXTRACT

    Hernawan Hernawan

    2010-06-01

    Full Text Available Hibiscus radiatus Cuv calyces extracts rich in polyphenols was screened for their potential to inhibit oxidation of human low-density lipoproteins-cholesterol (LDL-C in vitro. The inhibition of LDL-C oxidation (antioxidant activity was determined by measuring the formation of conjugated dienes and thiobarbituric acid reagent substances (TBARS. LDL-C oxidation was carried out in the presence of H. radiatus Cuv calyces extract (20 and 50 μM. CuSO4 (10 μM was used as the oxidation initiator and  butylated hydroxytoluene (BHT at 50 μM was used as standard antioxidant. The protective effect of H. radiatus Cuv. calyces extract toward human low-density lipoproteins, complex lipid system was  demonstrated by significant increase lag time (> 103 min, diminished of the propagation rate (44 %, and diminution of conjugated dienes formation 59.42 % (50 μM compared to control.   Keywords: antioxidant, conjugated dienes, Hibiscus radiatus Cuv, low-density lipoproteins-cholesterol

  3. Chemical-sensory properties and consumer preference of hibiscus beverages produced by improved industrial processes.

    Monteiro, Maria João P; Costa, Ana Isabel A; Fliedel, Geneviève; Cissé, Mady; Bechoff, Aurélie; Pallet, Dominique; Tomlins, Keith; Pintado, Maria Manuela E

    2017-06-15

    The need to increase sustainability and add value to traditional foods claiming health benefits led to the introduction of key improvements in the production of hibiscus beverages in Senegal. The physicochemical and sensory properties of three resulting products (an under-vacuum concentrate, a dilute-to-taste syrup and a ready-to-drink infusion) were assessed, vis-à-vis those of conventionally manufactured beverages, and their impact on local consumer preference determined (n=146). New beverages had more intense, redder colour and higher monomeric anthocyanin content, total phenolic content and antioxidant capacity. Moreover, their colour evaluations by trained panellists were mainly linked to colour density and anthocyanin/polyphenol content, while flavour assessments were associated to titratable acidity and sugar-to-acid ratio. Consumer evaluations, in turn, were driven by the beverages' red colour intensity, aroma strength and balance between sweetness and acidity. This explained why they overwhelmingly preferred the under-vacuum concentrate, regardless of their age, gender or frequency of hibiscus beverage consumption. Copyright © 2016 Elsevier Ltd. All rights reserved.

  4. Dietary fiber content and associated antioxidant compounds in Roselle flower (Hibiscus sabdariffa L.) beverage.

    Sáyago-Ayerdi, Sonia G; Arranz, Sara; Serrano, José; Goñi, Isabel

    2007-09-19

    The beverage of Hibiscus sabdariffa flowers is widely consumed in Mexico. Polyphenols contained in plant foods are frequently associated with dietary fiber. The aim of this work is to quantify the dietary fiber, associated polyphenols, and antioxidant capacity of the Roselle flower and the beverage traditionally prepared from it and its contribution to the Mexican diet. Roselle flower contained dietary fiber as the largest component (33.9%) and was rich in phenolic compounds (6.13%). Soluble dietary fiber was 0.66 g/L in beverage, and 66% of total extractable polyphenols contained in Roselle flower passed to the beverage and showed an antioxidant capacity of 335 micromoL trolox equivalents/100 mL beverage measured by ABTS. These data suggest that Roselle flower beverage intake in the Mexican diet may contribute around 166 and 165 mg/per serving to the intake of dietary fiber and polyphenols, respectively. The health benefits from consumption of Hibiscus beverage could be of considerable benefit to the whole population.

  5. Hibiscus syriacus Extract from an Established Cell Culture Stimulates Skin Wound Healing.

    di Martino, O; Tito, A; De Lucia, A; Cimmino, A; Cicotti, F; Apone, F; Colucci, G; Calabrò, V

    2017-01-01

    Higher plants are the source of a wide array of bioactive compounds that support skin integrity and health. Hibiscus syriacus , family Malvaceae, is a plant of Chinese origin known for its antipyretic, anthelmintic, and antifungal properties. The aim of this study was to assess the healing and hydration properties of H. syriacus ethanolic extract (HSEE). We established a cell culture from Hibiscus syriacus leaves and obtained an ethanol soluble extract from cultured cells. The properties of the extract were tested by gene expression and functional analyses on human fibroblast, keratinocytes, and skin explants. HSEE treatment increased the healing potential of fibroblasts and keratinocytes. Specifically, HSEE significantly stimulated fibronectin and collagen synthesis by 16 and 60%, respectively, while fibroblasts contractility was enhanced by 30%. These results were confirmed on skin explants, where HSEE accelerated the wound healing activity in terms of epithelium formation and fibronectin production. Moreover, HSEE increased the expression of genes involved in skin hydration and homeostasis. Specifically, aquaporin 3 and filaggrin genes were enhanced by 20 and 58%, respectively. Our data show that HSEE contains compounds capable of stimulating expression of biomarkers relevant to skin regeneration and hydration thereby counteracting molecular pathways leading to skin damage and aging.

  6. Hibiscus syriacus Extract from an Established Cell Culture Stimulates Skin Wound Healing

    O. di Martino

    2017-01-01

    Full Text Available Higher plants are the source of a wide array of bioactive compounds that support skin integrity and health. Hibiscus syriacus, family Malvaceae, is a plant of Chinese origin known for its antipyretic, anthelmintic, and antifungal properties. The aim of this study was to assess the healing and hydration properties of H. syriacus ethanolic extract (HSEE. We established a cell culture from Hibiscus syriacus leaves and obtained an ethanol soluble extract from cultured cells. The properties of the extract were tested by gene expression and functional analyses on human fibroblast, keratinocytes, and skin explants. HSEE treatment increased the healing potential of fibroblasts and keratinocytes. Specifically, HSEE significantly stimulated fibronectin and collagen synthesis by 16 and 60%, respectively, while fibroblasts contractility was enhanced by 30%. These results were confirmed on skin explants, where HSEE accelerated the wound healing activity in terms of epithelium formation and fibronectin production. Moreover, HSEE increased the expression of genes involved in skin hydration and homeostasis. Specifically, aquaporin 3 and filaggrin genes were enhanced by 20 and 58%, respectively. Our data show that HSEE contains compounds capable of stimulating expression of biomarkers relevant to skin regeneration and hydration thereby counteracting molecular pathways leading to skin damage and aging.

  7. Seasonal variation in Hibiscus sabdariffa (Roselle) calyx phytochemical profile, soluble solids and α-glucosidase inhibition.

    Ifie, Idolo; Ifie, Beatrice E; Ibitoye, Dorcas O; Marshall, Lisa J; Williamson, Gary

    2018-09-30

    Seasonal variations in crops can alter the profile and amount of constituent compounds and consequentially any biological activity. Differences in phytochemical profile, total phenolic content and inhibitory activity on α-glucosidase (maltase) of Hibiscus sabdariffa calyces grown in South Western Nigeria were determined over wet and dry seasons. The phenolic profile, organic acids and sugars were analysed using HPLC, while inhibition of rat intestinal maltase was measured enzymically. There was a significant increase (1.4-fold; p ≤ 0.05) in total anthocyanin content in the dry compared to wet planting seasons, and maltase inhibition from the dry season was slightly more potent (1.15-fold, p ≤ 0.05). Fructose (1.8-fold), glucose (1.8-fold) and malic acid (3.7-fold) were significantly higher (p ≤ 0.05) but citric acid was lower (62-fold, p ≤ 0.008) in the dry season. Environmental conditions provoke metabolic responses in Hibiscus sabdariffa affecting constituent phytochemicals and nutritional value. Copyright © 2018 Elsevier Ltd. All rights reserved.

  8. Anthocyanin-rich extract from Hibiscus sabdariffa calyx counteracts UVC-caused impairments in rats.

    Ozkol, Hatice Uce; Koyuncu, Ismail; Tuluce, Yasin; Dilsiz, Nihat; Soral, Sinan; Ozkol, Halil

    2015-01-01

    Ultraviolet radiation (UV) was reported to cause oxidative stress. Hibiscus sabdariffa L. (Malvaceae) calyx is commonly used in traditional Asian and African medicines and possesses strong antioxidant capacity due to its anthocyanin (ANTH) content. This study researched the possible protective role of Hibiscus sabdariffa calyx extract (HSCE) in UVC exposure of rats. Levels of serum enzymes, renal function tests, and some oxidant/antioxidant biomarkers of skin, lens, and retina tissues were monitored. Rats were exposed to UVC 4 h daily for 40 d and simultaneously received HSCE containing 2.5, 5, and 10 mg doses of ANTH in drinking water. Significant (p < 0.05) increases in the levels of serum aminotransferases, lactate dehydrogenase, urea, creatinine, and uric acid were noted after UVC exposure. In skin, lens, and retina tissues, total oxidant status, oxidative stress index, lipid peroxidation, and protein oxidation escalated markedly (p < 0.05) whereas total antioxidant status, reduced glutathione, and superoxide dismutase decreased dramatically (p < 0.05) related to UVC. Co-administration of HSCE with each ANTH dose significantly (p < 0.05) reversed aforementioned parameters (except total oxidant status) almost in all tissues. The LD50 of HSCE in rats was determined to be above 5000 mg/kg. Our data revealed that HSCE has a remarkable potential to counteract UVC-caused impairments, probably through its antioxidant and free radical-defusing effects. Therefore, HSCE could be useful against some cutaneous and ocular diseases in which UV and oxidative stress have a role in the etiopathogenesis.

  9. Ethyl acetate fraction from Hibiscus sabdariffa L. attenuates diabetes-associated cognitive impairment in mice.

    Seung, Tae Wan; Park, Seon Kyeong; Kang, Jin Yong; Kim, Jong Min; Park, Sang Hyun; Kwon, Bong Seok; Lee, Chang Jun; Kang, Jeong Eun; Kim, Dae Ok; Lee, Uk; Heo, Ho Jin

    2018-03-01

    The ameliorating effects of the ethyl acetate fraction from Hibiscus sabdariffa L. (EFHS) 2 against diabetes mellitus (DM) 3 and DM-induced cognitive impairment were investigated on streptozotocin (STZ) 4 -induced DM mice. The EFHS groups showed improved hyperglycemia and glucose tolerance compared to the STZ group. Furthermore, their liver and kidney function and lipid metabolic imbalance in the blood serum were effectively recovered. The EFHS groups significantly ameliorated STZ-induced cognitive impairment in Y-maze, passive avoidance, and Morris water maze (MWM) 5 tests. The EFHS groups showed significant improvement in the antioxidant and cholinergic systems of the brain tissue. In addition, EFHS had an excellent ameliorating effect on protein expression levels from the tau hyperphosphorylation pathways, such as phospho-c-Jun N-terminal kinases (p-JNK), 6 phospho-tau (p-tau), 7 and cleaved poly (ADP-ribose) polymerase (c-PARP). 8 The main compounds of EFHS were identified as various phenolic compounds, including hibiscus acid, caffeoylquinic acid (CQA) 9 isomers, and quercetin derivates. Therefore, EFHS containing various physiologically active materials can potentially be used for improving DM-induced cognitive impairment via its antioxidant activity, improvement of the cholinergic system, and hyperphosphorylation tau signaling. Copyright © 2017 Elsevier Ltd. All rights reserved.

  10. Disruption of Angiogenesis by Anthocyanin-Rich Extracts of Hibiscus sabdariffa

    Joshua, Madu; Okere, Christiana; Sylvester, O’Donnell; Yahaya, Muhammad; Precious, Omale; Dluya, Thagriki; Um, Ji-Yeon; Neksumi, Musa; Boyd, Jessica; Vincent-Tyndall, Jennifer; Choo, Dong-Won; Gutsaeva, Diana R.; Jahng, Wan Jin

    2017-01-01

    Abnormal vessel formations contribute to the progression of specific angiogenic diseases including age-related macular degeneration. Adequate vessel growth and maintenance represent the coordinated process of endothelial cell proliferation, matrix remodeling, and differentiation. However, the molecular mechanism of the proper balance between angiogenic activators and inhibitors remains elusive. In addition, quantitative analysis of vessel formation has been challenging due to complex angiogenic morphology. We hypothesized that conjugated double bond containing-natural products, including anthocyanin extracts from Hibiscus sabdariffa, may control the proper angiogenesis. The current study was designed to determine whether natural molecules from African plant library modulate angiogenesis. Further, we questioned how the proper balance of anti- or pro-angiogenic signaling can be obtained in the vascular microenvironment by treating anthocyanin or fatty acids using chick chorioallantoic membrane angiogenesis model in ovo. The angiogenic morphology was analyzed systematically by measuring twenty one angiogenic indexes using Angiogenic Analyzer software. Chick chorioallantoic model demonstrated that anthocyanin-rich extracts inhibited angiogenesis in time- and concentration-dependent manner. Molecular modeling analysis proposed that hibiscetin as a component in Hibiscus may bind to the active site of vascular endothelial growth factor receptor 2 (VEGFR2) with ΔG= −8.42 kcal/mol of binding energy. Our results provided the evidence that anthocyanin is an angiogenic modulator that can be used to treat uncontrolled neovascular-related diseases, including age-related macular degeneration. PMID:28459020

  11. Physiological and biochemical responses of Hibiscus sabdariffa to drought stress in the presence of salicylic acid

    Marzieh Mirshekari

    2017-08-01

    Salicylic acid (SA is one of the important signal molecules, which modulates plant responses to environmental stress. In the present work, impact of exogenous SA on some physiological and biochemical traits of Hibiscus sabdariffa in response to drought stress was studied. Hibiscus sabdariffa seedlings were exposed to six drought levels (0, -0.05, -0.1, -0.5, -0.75, and 1 MPa with two SA concentrations (0 and 500 µM in 5 days intervals up to 20 days in a factorial design. During drought stress period, the root and shoot growth, relative water content, pigments content, non-reducing sugar and starch content was significantly decreased. SA treatment cause prevention of the growth reduction and improvement of relative water content. Protein concentration was roughly unchanged during drought stress with SA, while, reducing sugars accumulates and non-reducing sugars and starch significantly decreases. The results show that exogenous SA application on leaves during drought stress can ameliorate detrimental effects of stress through reducing water loss and accumulating reducing sugars, which cause preserving turgor pressure of the cells.

  12. In vitro vasorelaxation mechanisms of bioactive compounds extracted from Hibiscus sabdariffa on rat thoracic aorta

    Sarr, Mamadou; Ngom, Saliou; Kane, Modou O; Wele, Alassane; Diop, Doudou; Sarr, Bocar; Gueye, Lamine; Andriantsitohaina, Ramaroson; Diallo, Aminata S

    2009-01-01

    Background In this study, we suggested characterizing the vasodilator effects and the phytochemical characteristics of a plant with food usage also used in traditional treatment of arterial high blood pressure in Senegal. Methods Vascular effects of crude extract of dried and powdered calyces of Hibiscus sabdariffa were evaluated on isolated thoracic aorta of male Wistar rats on organ chambers. The crude extract was also enriched by liquid-liquid extraction. The various cyclohexane, dichloromethane, ethyl acetate, butanol extracts obtained as well as the residual marc were subjected to Sephadex LH-20 column chromatography. The different methanolic eluate fractions were then analyzed by Thin Layer (TLC) and High Performance Liquid Chromatography (HPLC) and their vascular effects also evaluated. Results The H. Sabdariffa crude extract induced mainly endothelium-dependent relaxant effects. The endothelium-dependent relaxations result from NOS activation and those who not dependent to endothelium from activation of smooth muscle potassium channels. The phytochemical analysis revealed the presence of phenolic acids in the ethyl acetate extract and anthocyans in the butanolic extract. The biological efficiency of the various studied extracts, in term of vasorelaxant capacity, showed that: Butanol extract > Crude extract > Residual marc > Ethyl acetate extract. These results suggest that the strong activity of the butanolic extract is essentially due to the presence of anthocyans found in its fractions 43-67. Conclusion These results demonstrate the vasodilator potential of hibiscus sabdariffa and contribute to his valuation as therapeutic alternative. PMID:19883513

  13. COMPORTAMIENTO DEL CRECIMIENTO EN ALTURA DE Hibiscus elatus Sw CULTIVADA EN CONTENEDORES

    M. Cobas-López

    2003-01-01

    Full Text Available Hibiscus elatus Sw. (majagua es una especie nativa de Cuba, incluida en los planes de reforestación del país por su importancia económica, ecológica y su condición de ser una madera preciosa. En el trabajo se describe el comportamiento en vivero del crecimiento en altura de la especie, cultivada en contenedores. Se utilizaron cuatro tipos de sustratos, que fueron los siguientes: S1- Turba (40 %, humus de lombriz (40 % y corteza de pino compostada (20 %; S2- Estiércol de caballo (45 %, composta (40 %, y humus de lombriz (15 %; S3- Humus de lombriz (30 %, composta (25 %, turba (25 %, y estiércol de caballo (20 %; S4- Testigo, consistente en suelo proveniente de una plantación de la especie. A través del análisis de regresión se probaron nueve modelos matemáticos del tipo y = f (x, polinómicos, exponenciales y logarítmicos. De acuerdo con el coeficiente de determinación obtenido, del análisis de residuos y la validación de los modelos, se comprobó que la función que mejor representa el crecimiento en altura de Hibiscus elatus Sw., para los sustratos estudiados fue b t y b e 1 = 0 + .

  14. Determination of Suitable Microspore Stage and Callus Induction from Anthers of Kenaf (Hibiscus cannabinus L.

    Ahmed Mahmood Ibrahim

    2014-01-01

    Full Text Available Kenaf (Hibiscus cannabinus L. is one of the important species of Hibiscus cultivated for fiber. Availability of homozygous parent lines is prerequisite to the use of the heterosis effect reproducible in hybrid breeding. The production of haploid plants by anther culture followed by chromosome doubling can be achieved in short period compared with inbred lines by conventional method that requires self pollination of parent material. In this research, the effects of the microspore developmental stage, time of flower collection, various pretreatments, different combinations of hormones, and culture condition on anther culture of KB6 variety of Kenaf were studied. Young flower buds with immature anthers at the appropriate stage of microspore development were sterilized and the anthers were carefully dissected from the flower buds and subjected to various pretreatments and different combinations of hormones like NAA, 2,4-D, Kinetin, BAP, and TDZ to induce callus. The best microspore development stage of the flower buds was about 6–8 mm long collected 1-2 weeks after flower initiation. At that stage, the microspores were at the uninucleate stage which was suitable for culture. The best callus induction frequency was 90% in the optimized semisolid MS medium fortified with 3.0 mg/L BAP + 3.0 mg/L NAA.

  15. Cytotoxic activity of kenaf (Hibiscus cannabinus L.) seed extract and oil against human cancer cell lines

    Wong, Yu Hua; Tan, Wai Yan; Tan, Chin Ping; Long, Kamariah; Nyam, Kar Lin

    2014-01-01

    Objective To examine the cytotoxic properties of both the kenaf (Hibiscus cannabinus L.) seed extract and kenaf seed oil on human cervical cancer, human breast cancer, human colon cancer and human lung cancer cell lines. Methods The in vitro cytotoxic activity of the kenaf (Hibiscus cannabinus L.) seed extract and kenaf seed oil on human cancer cell lines was evaluated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and sulforhodamine B assays. Cell morphological changes were observed by using an inverted light microscope. Results The kenaf seed extract (KSE) exhibited a lower IC50 than kenaf seed oil (KSO) in all of the cancer cell lines. Morphological alterations in the cell lines after KSE and KSO treatment were observed. KSE and KSO possessed effective cytotoxic activities against all the cell lines been selected. Conclusions KSE and KSO could be potential sources of natural anti-cancer agents. Further investigations on using kenaf seeds for anti-proliferative properties are warranted. PMID:25183141

  16. Determination of Suitable Microspore Stage and Callus Induction from Anthers of Kenaf (Hibiscus cannabinus L.)

    Binti Kayat, Fatimah; Ermiena Surya Mat Hussin, Zeti; Susanto, Dwi; Ariffulah, Mohammed

    2014-01-01

    Kenaf (Hibiscus cannabinus L.) is one of the important species of Hibiscus cultivated for fiber. Availability of homozygous parent lines is prerequisite to the use of the heterosis effect reproducible in hybrid breeding. The production of haploid plants by anther culture followed by chromosome doubling can be achieved in short period compared with inbred lines by conventional method that requires self pollination of parent material. In this research, the effects of the microspore developmental stage, time of flower collection, various pretreatments, different combinations of hormones, and culture condition on anther culture of KB6 variety of Kenaf were studied. Young flower buds with immature anthers at the appropriate stage of microspore development were sterilized and the anthers were carefully dissected from the flower buds and subjected to various pretreatments and different combinations of hormones like NAA, 2,4-D, Kinetin, BAP, and TDZ to induce callus. The best microspore development stage of the flower buds was about 6–8 mm long collected 1-2 weeks after flower initiation. At that stage, the microspores were at the uninucleate stage which was suitable for culture. The best callus induction frequency was 90% in the optimized semisolid MS medium fortified with 3.0 mg/L BAP + 3.0 mg/L NAA. PMID:24757416

  17. Comparison of leaf anatomical characteristics of hibiscus rosa-sinensis grown in faisalabad region

    Noman, A.; Ali, Q.; Mehmood, T.; Iftikhar, T.; Mahmeed, M.

    2014-01-01

    The genetic potential of different plant species to different environmental conditions differ in relation to different physiological, biochemical and anatomical characteristics. Of these varying attributes leaf anatomical characteristics play most important role for the establishment of that cultivar in varied environmental conditions. So, the present study was conducted to assess the inter-cultivar genetic potential of Hibiscus in relation to leaf anatomical characteristics. To fulfill the study requirements Hibiscus rosa-sinensis and its six cultivars (were well adapted to their specific natural habitat) were collected from different locations of district Faisalabad Pakistan that have great environmental changes round the year. Results showed significant variability among cultivars in relation to analyzed anatomical characteristics. Cultivars Lemon shiffon and Wilder's white emerge more promising among others by possessing more epidermal thickness, increased epidermal cell area, high cortical cell area and incremented stomatal density as compared with other cultivars. On the other hand, cultivars Cooperi alba, Mrs. George Davis and Frank green possessed least cortex cell area, lowest xylem region thickness and minimum phloem region thickness respectively. Overall, it can be concluded that anatomical genetic potential has endorsed cultivars Lemon chiffon and Wilder's white with enormous capability to grow well under variable environments. (author)

  18. Lepidopterans and their parasitoids on okra plants in Riberão Preto (SP, Brazil Lepidópteros e seus parasitoides em cultura de quiabo em Ribeirão Preto, SP, Brasil

    Rogéria Inês Rosa Lara

    2012-04-01

    Full Text Available The aim of this study was to identify the defoliating lepidopterans and their parasitoids occurring on okra plants in the district of Riberão Preto city (SP. Brazil. Caterpillars were collected by hand from an experimental field of okra, in March and April, 2009. They were placed individually on Petri dishes lined with wet filter paper, containing shredded okra leaves, and kept in a climactic chamber (25±1°C, 12:12h light/dark photoperiod, RH 70±10% until the emergence of lepidopterans and/or parasitoids. In all, 63 lepidopterans were obtained: three species of Noctuidae, Anomis flava (Fabricius, Spodoptera frugiperda (Smith and Pseudoplusia includens (Walker; one of Pieridae, Ascia monuste orseis (Godart, and an unidentified species of Tortricidae. One unidentified tachinid fly (Diptera and the Hymenoptera parasitoids Copidosoma floridanum (Ashmead (Encyrtidae and Euplectrus sp. (Eulophidae emerged from A. flava, while C. floridanum and an unidentified tachinid, from P. includens. This is the first report of an association between the herbivores S. frugiperda, P. includens and A. monuste orceis, the parasitoids C. floridanum and Euplectrus sp. on okra plants.Este estudo objetivou identificar lepidópteros desfolhadores e seus parasitoides em cultura de quiabo em Ribeirão Preto, SP, Brasil. As lagartas foram coletadas através de catação manual entre março e abril de 2009, individualizadas em placas de Petri forradas com papel filtro umedecido contendo pedaços de folhas de quiabo e mantidas sob condições controladas (25±1°C, 12 horas de fotofase, 70±10% de UR até a obtenção dos adultos de lepidópteros e/ou de seus parasitoides. Foram obtidos 63 adultos de lepidópteros: três espécies de Noctuidae, Anomis flava (Fabricius, Spodoptera frugiperda (Smith, Pseudoplusia includens (Walker, uma de Pieridae, Ascia monuste orseis (Godart e uma de Tortricidae não identificada. Uma espécie não identificada de taquinídeo (Diptera e os

  19. Effect of Absolute From Hibiscus syriacus L. Flower on Wound Healing in Keratinocytes

    Yoon, Seok Won; Lee, Kang Pa; Kim, Do-Yoon; Hwang, Dae Il; Won, Kyung-Jong; Lee, Dae Won; Lee, Hwan Myung

    2017-01-01

    Background: Proliferation and migration of keratinocytes are essential for the repair of cutaneous wounds. Hibiscus syriacus L. has been used in Asian medicine; however, research on keratinocytes is inadequate. Objective: To establish the dermatological properties of absolute from Hibiscus syriacus L. flower (HSF) and to provide fundamental research for alternative medicine. Materials and Methods: We identified the composition of HSF absolute using gas chromatography-mass spectrometry analysis. We also examined the effect of HSF absolute in HaCaT cells using the XTT assay, Boyden chamber assay, sprout-out growth assay, and western blotting. We conducted an in-vivo wound healing assay in rat tail-skin. Results: Ten major active compounds were identified from HSF absolute. As determined by the XTT assay, Boyden chamber assay, and sprout-out growth assay results, HSF absolute exhibited similar effects as that of epidermal growth factor on the proliferation and migration patterns of keratinocytes (HaCaT cells), which were significantly increased after HSF absolute treatment. The expression levels of the phosphorylated signaling proteins relevant to proliferation, including extracellular signal-regulated kinase 1/2 (Erk 1/2) and Akt, were also determined by western blot analysis. Conclusion: These results of our in-vitro and ex-vivo studies indicate that HSF absolute induced cell growth and migration of HaCaT cells by phosphorylating both Erk 1/2 and Akt. Moreover, we confirmed the wound-healing effect of HSF on injury of the rat tail-skin. Therefore, our results suggest that HSF absolute is promising for use in cosmetics and alternative medicine. SUMMARY Hisbiscus syriacus L. flower absolute increases HaCaT cell migration and proliferation.Hisbiscus syriacus L. flower absolute regulates phosphorylation of ERK 1/2 and Akt in HaCaT cell.Treatment with Hisbiscus syriacus L. flower induced sprout outgrowth.The wound in the tail-skin of rat was reduced by Hisbiscus syriacus

  20. Inhibiton of Yellow Nutsedge (Cyperus esculentus L. and Bermudagrass (Cynodon dactylon (L. Pers by a Mulch Derived from Rye (Secale cereale L. in grapevines Inhibición del Crecimiento de Chufa (Cyperus esculentus L. y Pasto Bermuda (Cynodon dactylon (L. Pers. con mulch Vegetal Proveniente de Centeno (Secale cereale L. en Vides

    Juan Ormeño-Núñez

    2008-09-01

    Full Text Available Two field trials (Los Andes 1998-1999 and Santiago 2004-2005 were carried out to determine growth inhibition of yellow nutsedge (Cyperus esculentus L. and bermudagrass (Cynodon dactylon (L. Pers., growing on the plantation row, by mulch derived from a rye (Secale cereale L. cover crop established between grapevine (Vitis vinifera L. rows on overhead (cv. Flame Seedless and vertical (cv. Cabernet Sauvignon training. Spring mowing of the rye sown in the fall allowed for developing a thick and long lasting mulch along the grape rows. Nutsedge and bermudagrass control was 81 and 82%, respectively, and was more effective than conventional chemical (in the row + mechanical (between rows control. Glyphosate at 2% for nutsedge and 1% for bermudagrass control, applied twice (October and December, was insufficient to control either perennial weed adequately. Total broadleaved and grass/sedge weed control was 67.3 and 43.0% more effective with the rye mulch than with conventional treatments at Los Andes and Santiago, respectively. Perennial weed control levels could be explained as the new foliage of yellow nutsedge and bermudagrass was particularly susceptible to the shading provided by the rye mulch assembled prior to mid spring shoot emergence, and this effect remained active up until the beginning of autumn. The subsequent rye foliage mowing at the vegetative stage fully expressed the allelopathic effect produced by this local rye cultivar. The use of rye cover crop management and mulch could be applied as an effective weed control technique in conventional, as well as organic deciduous tree orchards.En dos ensayos de campo (Los Andes 1998-1999 y Santiago 2004-2005 se determinó el efecto inhibitorio sobre chufa (Cyperus esculentus L. y pasto bermuda (Cynodon dactylon (L. Pers. de residuos de centeno (Secale cereale L. establecido en otoño entre las hileras de vides (Vitis vinifera L. en parronal (cv. Flame Seedless y espaldera (cv. Cabernet Sauvignon

  1. Effect of endophytic pseudomonas aeruinosa and trichoderma harzianum on soil-borne diseases, mycorrhizae and induction of systemic resistance in okra grown in soil amended with vernonia anthelmintica (L.) seeds powder

    Shafique, H. A.; Noreen, R.; Haque, S. E. U.; Sultana, V.; Ara, J.

    2015-01-01

    Biostimulants are used in agricultural practices for plant growth improvement. These fertilizers improve microbial activity and cause a negative impact on soil-borne pathogens. In recent years, stimulating plant natural defense is considered as most promising alternative strategy for crop productivity. The present study was carried out to examine the effect of endophytic Pseudomonas aeruginosa and Trichoderma harzianum in soil amendment with Vernonia anthelmintica seeds powder, on root rotting fungi, plant growth, mycorrhizal population around roots, phosphorous uptake and stimulation of plant defense markers like poylphenol and antioxidant status in okra. Combine application of Vernonia with Pseudomonas aeruginosa and Trichoderma harzianum significantly (p<0.05) suppressed Rhizoctonia solani and Fusarium oxysporum with complete reduction of Macrophomina phaseolina and Fusarium solani. Pseudomonas aeruginosa and T. harzianum alone or in Vernonia amended soil significantly reduced nematode galls on roots. Organic amendment also improved plant resistance against root diseases as evident from enhanced DPPH radical scavenging capacity and polyphenol content in treated plants as compare to control. VA Mycorrhizal spores were found significantly (p<0.05) higher in number around roots received Pseudomonas aeruginosa or T. harzianum alone or in Vernonia amended soil. Whereas, higher concentrations of phosphorus in okra shoots were found in plants received biocontrol agents in amended soil. Mixed application of PGPR and T. harzianum in amended soil produced tallest plants than other treatments. Soil amendment with Vernonia seed powder alone or with biocontrol agents offer a non-chemical means of plant disease control. (author)

  2. Effects of 60Co γ-rays irradiation on germination and seedling growth of Hibiscus syriacus

    Li Xiufen; Wu Fulan; Zhang Deshun; Meng Zhennong; Cao Jiyun

    2009-01-01

    The seeds of Hibiscus syria cus were irradiated by 60 Co γ-rays at dose rate of 50 ∼ 500Gy/h for 0.5 and 1h, the seed germination rate and the seedling growth characters were surveyed. The results indicated that the seed germination was promoted when the absorbed dose was below 200Gy, and the seedling survival rate was increased when the dose was below 100Gy. The germination was inhibited, leaves and secondary roots were difficult to form, and the seedlings blasting occurred when the dose was over 200Gy. The dose rate affects the irradiation results. Absorbed dosed of 50 ∼ 100Gy were suggested for increasing seedling growth rate and 100 ∼ 200Gy were recommend for mutagenesis. (authors)

  3. Cytotoxic effect of triterpenoids from the root bark of Hibiscus syriacus.

    Shi, Li-Shian; Wu, Chao-Hsuan; Yang, Te-Chun; Yao, Chen-Wen; Lin, Hang-Ching; Chang, Wen-Liang

    2014-09-01

    In this study, 4 new triterpenoids-3β- acetoxy-olean-11-en,28,13β-olide (1), 3β- acetoxy-11α,12α-epoxy-olean-28,13β-olide (2), 19α-epi-betulin (3), and 20, 28-epoxy-17β,19β-lupan-3β-ol (4)-and 12 known compounds, were isolated from the root bark of Hibiscus syriacus L. by using acetone extraction. Their structures were characterized by extensive spectroscopic analysis. To investigate cytotoxicity, A549 human lung cancer cells were exposed to the extract and the compounds identified from it. Significantly reduced cell viability was observed with betulin-3-caffeate (12) (IC50, 4.3 μM). The results of this study indicate that betulin-3-caffeate (12) identified from H. syriacus L. may warrant further investigation for potential as anticancer therapies. Copyright © 2014 Elsevier B.V. All rights reserved.

  4. Hibiscus sabdariffa L extract drying with different carrier agent: Drying rate evaluation and color analysis

    Djaeni, M.; Utari, F. D.; Kumoro, A. C.

    2017-03-01

    The aim of this study was to investigate the effect of different carrier agents on roselle or Hibiscus sabdariffa L.extract drying. Carrier agent was used for reducing stickiness of material and avoiding agglomeration as well as improving stability. The method consisted of two steps involving roselle extraction and drying process. The liquid roselle extract was mixed with carrier agent (maltodextrin-arabic gum) in various composition. The mixture was then dried at different air temperature ranging 40 - 80°C. As a response, moisture content in the extract was observed by gravimetry every 10 minutes during90 minutes. The procedurewas repeated for the drying without carrieragent. The result showed that constant rate of drying with carrier agent was higher up to l.7 times than that of without carrier agent. Based on the color analysis,roselle extract drying with carrier agent also showed reasonable quality.

  5. Variability of cirrus clouds in a convective outflow during the Hibiscus campaign

    Fierli, F.; di Donfrancesco, G.; Cairo, F.; Marécal, V.; Zampieri, M.; Orlandi, E.; Durry, G.

    2008-08-01

    Light-weight microlidar and water vapour measurements were taken on-board a stratospheric balloon during the HIBISCUS 2004 campaign, held in Bauru, Brazil (49° W, 22° S). Cirrus clouds were observed throughout the flight between 12 and 15 km height with a high mesoscale variability in optical and microphysical properties. It was found that the cirrus clouds were composed of different layers characterized by marked differences in height, thickness and optical properties. Simultaneous water vapour observations show that the different layers are characterized by different values of the saturation with respect to ice. A mesoscale simulation and a trajectory analysis clearly revealed that the clouds had formed in the outflow of a large and persistent convective region and that the observed variability of the optical properties and of the cloud structure is likely linked to the different residence times of the convectively-processed air in the upper troposphere.

  6. Synthesis of monodispersed silver nanoparticles using Hibiscus cannabinus leaf extract and its antimicrobial activity

    Bindhu, M. R.; Umadevi, M.

    2013-01-01

    Synthesis of silver nanoparticles using leaf extract of Hibiscus cannabinus has been investigated. The influences of different concentration of H. cannabinus leaf extract, different metal ion concentration and different reaction time on the above cases on the synthesis of nanoparticles were evaluated. The synthesized nanoparticles were characterized using UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Transmission Electron Microscopy (TEM). The prepared silver nanoparticles were monodispersed, spherical in shape with the average particle size of 9 nm and shows surface plasmon peak at 446 nm. The study also reveals that the ascorbic acid present in H. cannabinus leaf extract has been used as reducing agent. The prepared silver nanoparticle shows good antimicrobial activity against Escherichia coli, Proteus mirabilis and Shigella flexneri.

  7. Flower color changes in three Japanese hibiscus species: further quantitative variation of anthocyanin and flavonols.

    Shimokawa, Satoshi; Iwashina, Tsukasa; Murakami, Noriaki

    2015-03-01

    One anthocyanin and four flavonols were detected from the petals of Hibiscus hamabo, H. tiliaceus and H. glaber. They were identified as cyanidin 3-0- sambubioside, gossypetin 3-O-glucuronide-8-O-glucoside, quercetin 7-O-rutinoside, gossypetin 3-O-glucoside and gossypetin 8-O-glucuronide by UV spectra, LC-MS, acid hydrolysis and HPLC. The flavonoid composition was essentially the same among the petals ofH. hamabo, H. tiliaceus and H. glaber, and there was little quantitative variation, except for cyanidin 3-O-sambubioside, the content of which in the petals ofH. tiliaceus and H. glaber was much higher than in that of H. hamabo. Flower colors of H. tiliaceus and H. glaber change from yellow to red, and that of H. hamabo changes from yellow to orange. These changes were caused by contents of anthocyanin and flavonols, which increased after flowering of H. hamabo, H. tiliaceus and H. glaber.

  8. OPTIMIZATION OF HIBISCUS SABDARIFFA L. (ROSELLE ANTHOCYANIN AQUEOUS-ETHANOL EXTRACTION PARAMETERS USING RESPONSE SURFACE METHODOLOGY

    ANILÚ MIRANDA-MEDINA

    2018-03-01

    Full Text Available Anthocyanins along with protocatechuic acid and quercetin have been recognized as bioactive compounds in Hibiscus sabdariffa L. aqueous extracts. Characteristic anthocyanin absorption in the visible region makes their quantification possible without the interference of the other two compounds, and also can favor its potential application as an alternative to organic-based dye sensitized solar cell, in various forms. In order to optimize measurable factors linked to the extraction of these flavonoids, an optimization was performed using a Box-Behnken experimental design and response surface methodology (RSM. Three levels of ethanol concentration, temperature and solid-solvent ratio (SSR were investigated. The optimization model showed that with 96 % EtOH, 65 °C, and 1:50 SSR, the highest anthocyanin concentration of 150 mg/100 g was obtained.

  9. A Stylistic Analysis of Linguistic Patterns in Chichamanda Ngozi Adichie’s Purple Hibiscus

    Muchamad Sholakhuddin Al Fajri

    2017-06-01

    Full Text Available This study aims to carry out a detailed and systematic stylistic analysis of linguistic patterns in Purple Hibiscus Novel by Chichamanda Ngozi Adichie. It particularly analyses a specific extract of the novel in terms of narration and point of view, conversational analysis, speech and thought presentations and mind style, and how these linguistic devices and patterns are employed by the author to shape characters’ personalities and relationships between them in the reader’s mind. The result appears to suggest that the author successfully represents the protagonist, Kambili as an obedient and a salient daughter who respects deeply his father, while her father, Eugene, is constructed as a strict father and religious who imposes an absolute control on his daughter.

  10. Physicochemical Properties and Oxidative Storage Stability of Milled Roselle (Hibiscus sabdariffa L. Seeds

    Nurul Hanisah Juhari

    2018-02-01

    Full Text Available Milled Roselle (Hibiscus sabdariffa L. seeds of the UMKL cultivar were analyzed for proximate composition, water and oil absorption capacity, and the influence of storage conditions on storage stability. The storage stability was determined under four types of conditions: light/oxygen (air (LO, light/nitrogen (LN, darkness/oxygen (air (DO, and darkness/nitrogen (DN while monitoring for seven consecutive months. During the storage period, the formation of volatiles was determined using dynamic headspace sampling and Gas Chromatography-Mass Spectrometry (GC-MS analysis. In total, 85 volatiles were identified, mainly aldehydes, alcohols, ketones, furans, and acids indicating lipid oxidation. It is recommended that milled Roselle seeds should be flushed with nitrogen and stored in darkness. Under these conditions, the seeds can be stored for at least three months without changes in volatile profile. This is important to ensure the good quality of milled Roselle seeds for further commercialization.

  11. Physicochemical Properties and Oxidative Storage Stability of Milled Roselle (Hibiscus sabdariffa L.) Seeds.

    Juhari, Nurul Hanisah; Petersen, Mikael Agerlin

    2018-02-11

    Milled Roselle ( Hibiscus sabdariffa L.) seeds of the UMKL cultivar were analyzed for proximate composition, water and oil absorption capacity, and the influence of storage conditions on storage stability. The storage stability was determined under four types of conditions: light/oxygen (air) (LO), light/nitrogen (LN), darkness/oxygen (air) (DO), and darkness/nitrogen (DN) while monitoring for seven consecutive months. During the storage period, the formation of volatiles was determined using dynamic headspace sampling and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. In total, 85 volatiles were identified, mainly aldehydes, alcohols, ketones, furans, and acids indicating lipid oxidation. It is recommended that milled Roselle seeds should be flushed with nitrogen and stored in darkness. Under these conditions, the seeds can be stored for at least three months without changes in volatile profile. This is important to ensure the good quality of milled Roselle seeds for further commercialization.

  12. Antibacterial efficiency of the Sudanese Roselle (Hibiscus sabdariffa L.), a famous beverage from Sudanese folk medicine.

    Abdallah, Emad Mohamed

    2016-01-01

    Hibiscus sabdariffa L. is a plant native to tropical Africa and intensively cultivated in Sudan. Its calyces are widely consumed with many uses in Sudanese folk medicine. The dried calyces of H. sabdariffa were subjected to soak in 80% v/v methanol to get the methanolic extract, which was tested against five Gram-negative and three Gram-positive referenced bacterial strains using disc diffusion method. Selected bioactive phytochemical compounds were also investigated using qualitative methods. The results of the antibacterial test indicate that the methanol extract of H. sabdariffa calyces contained effective antibacterial agent(s), revealed a considerable zone of inhibition against all tested Gram-negative and Gram-positive bacteria, and it was a competitor to gentamicin and greatly higher than penicillin which showed weak or no effect. The results of current investigation support the folk medicine application of this plant against different microbial ailments and suggest it as a promising source for new antibacterial agents.

  13. Nonlinear refractive index measurements and self-action effects in Roselle-Hibiscus Sabdariffa solutions

    Henari, F. Z.; Al-Saie, A.

    2006-12-01

    We report the observation of self-action phenomena, such as self-focusing, self-defocusing, self-phase modulation and beam fanning in Roselle-Hibiscus Sabdariffa solutions. This material is found to be a new type of natural nonlinear media, and the nonlinear reflective index coefficient has been determined using a Z-scan technique and by measuring the critical power for the self-trapping effect. Z-scan measurements show that this material has a large negative nonlinear refractive index, n 2 = 1 × 10-4 esu. A comparison between the experimental n 2 values and the calculated thermal value for n 2 suggests that the major contribution to nonlinear response is of thermal origin.

  14. Synthesis of monodispersed silver nanoparticles using Hibiscus cannabinus leaf extract and its antimicrobial activity.

    Bindhu, M R; Umadevi, M

    2013-01-15

    Synthesis of silver nanoparticles using leaf extract of Hibiscus cannabinus has been investigated. The influences of different concentration of H. cannabinus leaf extract, different metal ion concentration and different reaction time on the above cases on the synthesis of nanoparticles were evaluated. The synthesized nanoparticles were characterized using UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Transmission Electron Microscopy (TEM). The prepared silver nanoparticles were monodispersed, spherical in shape with the average particle size of 9 nm and shows surface plasmon peak at 446 nm. The study also reveals that the ascorbic acid present in H. cannabinus leaf extract has been used as reducing agent. The prepared silver nanoparticle shows good antimicrobial activity against Escherichia coli, Proteus mirabilis and Shigella flexneri. Copyright © 2012 Elsevier B.V. All rights reserved.

  15. Biosynthesis, characterisation and antimicrobial activity of silver nanoparticles using Hibiscus rosa-sinensis petals extracts.

    Nayak, Debasis; Ashe, Sarbani; Rauta, Pradipta Ranjan; Nayak, Bismita

    2015-10-01

    Green synthesis of metallic nanoparticles has lured the world from the chemical and physical approaches owing to its rapid, non-hazardous and economic aspect of production mechanism. In this study, silver nanoparticles (AgNPs) were synthesised using petal extracts of Hibiscus rosa-sinensis. The AgNPs displayed characteristic surface plasmon resonance peak at around 421 nm having a mean particle size of 76.25±0.17 nm and carried a charge of -41±0.2 mV. The X-ray diffraction patterns displayed typical peaks of face centred cubic crystalline silver. The surface morphology was characterised by scanning electron microscopy and atomic force microscopy. Fourier transform infrared spectroscopy studies confirmed the surface modifications of the functional groups for the synthesis of AgNPs. Furthermore, the synthesised AgNPs displayed proficient antimicrobial activity against pathogenic strains of Vibrio cholerae, Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus.

  16. Physiological responses and tolerance of kenaf (Hibiscus cannabinus L.) exposed to chromium.

    Ding, Han; Wang, Guodong; Lou, Lili; Lv, Jinyin

    2016-11-01

    Selection of kenaf species with chromium (Cr) tolerance and exploring the physiological mechanisms involved in Cr tolerance are crucial for application of these species to phyto-remediation. In the present study, a hydroponic experiment was conducted to investigate the variation in two kenaf cultivars, K39-2 and Zhe50-3 under Cr stress. At the same Cr concentration, the tolerance index (TI) of K39-2 was higher than that of Zhe50-3, indicating that K39-2 may be more tolerant to Cr than Zhe50-3. It was also observed that high concentration of chromium was accumulated both in the shoots and the roots of Hibiscus cannabinus L. The leaves of K39-2 accumulated 4760.28mgkg(-1) of dry weight under 1.50mM Cr stress, and the roots accumulated 11,958.33mgkg(-1). Physiological response shows that the antioxidant enzymes' superoxide dismutase (SOD), catalase activity (CAT) and peroxidase (POD) activities increased in the leaves and decreased in roots of the Cr-stressed plants nearly compared to the control. Moreover, the variation of antioxidant enzymes activities indicated Zhe50-3 was more vulnerable than K39-2, and the contents of the non-protein thiol pool (GSH, NPT and PCs) were higher in K39-2 than Zhe50-3 with the increased Cr concentration. Based on the observations above, it can be concluded that the well-coordinated physiological changes confer a greater Cr tolerance to K39-2 than Zhe50-3 under Cr exposure, and Hibiscus cannabinus L. has a great accumulation capacity for chromium. Copyright © 2016 Elsevier Inc. All rights reserved.

  17. Preliminary X-ray data analysis of crystalline hibiscus chlorotic ringspot virus

    Cheng, Ao; Speir, Jeffrey A.; Yuan, Y. Adam; Johnson, John E.; Wong, Sek-Man

    2009-01-01

    Hibiscus chlorotic ringspot virus is a positive-sense monopartite single-stranded RNA virus that belongs to the Carmovirus genus of the Tombusviridae family. Authentic virus harvested from infected host kenaf leaves was purified and virus crystals were grown in multiple conditions. One of the crystals diffracted to 3.2 Å resolution and allowed the collection of a partial data set. Hibiscus chlorotic ringspot virus (HCRSV) is a positive-sense monopartite single-stranded RNA virus that belongs to the Carmovirus genus of the Tombusviridae family, which includes carnation mottle virus (CarMV). The HCRSV virion has a 30 nm diameter icosahedral capsid with T = 3 quasi-symmetry containing 180 copies of a 38 kDa coat protein (CP) and encapsidates a full-length 3.9 kb genomic RNA. Authentic virus was harvested from infected host kenaf leaves and was purified by saturated ammonium sulfate precipitation, sucrose density-gradient centrifugation and anion-exchange chromatography. Virus crystals were grown in multiple conditions; one of the crystals diffracted to 3.2 Å resolution and allowed the collection of a partial data set. The crystal belonged to space group R32, with unit-cell parameters a = b = 336.4, c = 798.5 Å. Packing considerations and rotation-function analysis determined that there were three particles per unit cell, all of which have the same orientation and fixed positions, and resulted in tenfold noncrystallography symmetry for real-space averaging. The crystals used for the structure determination of southern bean mosaic virus (SBMV) have nearly identical characteristics. Together, these findings will greatly aid the high-resolution structure determination of HCRSV

  18. Aqueous Extracts of Hibiscus sabdariffa Calyces Decrease Hepatitis A Virus and Human Norovirus Surrogate Titers.

    Joshi, Snehal S; Dice, Lezlee; D'Souza, Doris H

    2015-12-01

    Hibiscus sabdariffa extract is known to have antioxidant, anti-diabetic, and antimicrobial properties. However, their effects against foodborne viruses are currently unknown. The objective of this study was to determine the antiviral effects of aqueous extracts of H. sabdariffa against human norovirus surrogates (feline calicivirus (FCV-F9) and murine norovirus (MNV-1)) and hepatitis A virus (HAV) at 37 °C over 24 h. Individual viruses (~5 log PFU/ml) were incubated with 40 or 100 mg/ml of aqueous hibiscus extract (HE; pH 3.6), protocatechuic acid (PCA; 3 or 6 mg/ml, pH 3.6), ferulic acid (FA; 0.5 or 1 mg/ml; pH 4.0), malic acid (10 mM; pH 3.0), or phosphate buffered saline (pH 7.2 as control) at 37 °C over 24 h. Each treatment was replicated thrice and plaque assayed in duplicate. FCV-F9 titers were reduced to undetectable levels after 15 min with both 40 and 100 mg/ml HE. MNV-1 was reduced by 1.77 ± 0.10 and 1.88 ± 0.12 log PFU/ml after 6 h with 40 and 100 mg/ml HE, respectively, and to undetectable levels after 24 h by both concentrations. HAV was reduced to undetectable levels by both HE concentrations after 24 h. PCA at 3 mg/ml reduced FCV-F9 titers to undetectable levels after 6 h, MNV-1 by 0.53 ± 0.01 log PFU/ml after 6 h, and caused no significant change in HAV titers. FA reduced FCV-F9 to undetectable levels after 3 h and MNV-1 and HAV after 24 h. Transmission electron microscopy showed no conclusive results. The findings suggest that H. sabdariffa extracts have potential to prevent foodborne viral transmission.

  19. Development and evaluation of Ketoprofen sustained release matrix tablet using Hibiscus rosa-sinensis leaves mucilage

    M. Kaleemullah

    2017-07-01

    Full Text Available Currently, the use of natural gums and mucilage is of increasing importance in pharmaceutical formulations as valuable drug excipient. Natural plant-based materials are economic, free of side effects, biocompatible and biodegradable. Therefore, Ketoprofen matrix tablets were formulated by employing Hibiscus rosa-sinensis leaves mucilage as natural polymer and HPMC (K100M as a synthetic polymer to sustain the drug release from matrix system. Direct compression method was used to develop sustained released matrix tablets. The formulated matrix tablets were evaluated in terms of physical appearance, weight variation, thickness, diameter, hardness, friability and in vitro drug release. The difference between the natural and synthetic polymers was investigated concurrently. Matrix tablets developed from each formulation passed all standard physical evaluation tests. The dissolution studies of formulated tablets revealed sustained drug release up to 24 h compared to the reference drug Apo Keto® SR tablets. The dissolution data later were fitted into kinetic models such as zero order equation, first order equation, Higuchi equation, Hixson Crowell equation and Korsmeyer-Peppas equation to study the release of drugs from each formulation. The best formulations were selected based on the similarity factor (f2 value of 50% and more. Through the research, it is found that by increasing the polymers concentration, the rate of drug release decreased for both natural and synthetic polymers. The best formulation was found to be F3 which contained 40% Hibiscus rosa-sinensis mucilage polymer and showed comparable dissolution profile to the reference drug with f2 value of 78.03%. The release kinetics of this formulation has shown to follow non-Fickian type which involved both diffusion and erosion mechanism. Additionally, the statistical results indicated that there was no significant difference (p > 0.05 between the F3 and reference drug in terms of MDT and

  20. Beneficial effects of Hibiscus rosa-sinensis L. flower aqueous extract in pregnant rats with diabetes.

    Afiune, Luana Alves Freitas; Leal-Silva, Thaís; Sinzato, Yuri Karen; Moraes-Souza, Rafaianne Queiroz; Soares, Thaigra Sousa; Campos, Kleber Eduardo; Fujiwara, Ricardo Toshio; Herrera, Emilio; Damasceno, Débora Cristina; Volpato, Gustavo Tadeu

    2017-01-01

    The Hibiscus rosa-sinensis flower is widely used in Brazilian traditional medicine for the treatment of diabetes and has shown antifertility activity in female Wistar rats. However, there is no scientific confirmation of its effect on diabetes and pregnancy. The aim of this study was evaluate the effect of aqueous extract of H. rosa-sinensis flowers on maternal-fetal outcome in pregnant rats with diabetes. Diabetes was induced by streptozotocin (STZ, 40 mg/kg) in virgin, adult, female Wistar rats. After diabetes induction, the rats were mated. The pregnant rats were distributed into four groups (n minimum = 11 animals/group): non-diabetic, non-diabetic treated, diabetic, and diabetic treated. Oral aqueous extract of Hibiscus rosa-sinensis was administered to rats in the treatment groups during pregnancy. At term pregnancy, maternal reproductive outcomes, fetal parameters, and biochemical parameters were analyzed. The non-diabetic treated group showed decreased high density lipoprotein cholesterol, increased atherogenic index (AI) and coronary artery risk index (CRI), and increased preimplantation loss rate compared to the non-diabetic group. Although treatment with H. rosa-sinensis led to no toxicity, it showed deleterious effects on cardiac and reproductive functions. However, the diabetic treated group showed increased maternal and fetal weights, reduced AI and CRI, and reduced preimplantation loss rate compared to the untreated diabetic group. Our results demonstrate beneficial effects of this flower only in pregnant rats with diabetes and their offspring. Although these findings cannot be extrapolated to human clinical use, they show that the indiscriminate intake of H. rosa-sinensis may be harmful to healthy individuals and its use should be completely avoided in pregnancy.

  1. Hibiscus anthocyanins rich extract-induced apoptotic cell death in human promyelocytic leukemia cells

    Chang, Y.-C.; Huang, H.-P.; Hsu, J.-D.; Yang, S.-F.; Wang, C.-J.

    2005-01-01

    Hibiscus sabdariffa Linne (Malvaceae), an attractive plant believed to be native to Africa, is cultivated in the Sudan and Eastern Taiwan. Anthocyanins exist widely in many vegetables and fruits. Some reports demonstrated that anthocyanins extracted from H. sabdariffa L., Hibiscus anthocyanins (HAs) (which are a group of natural pigments existing in the dried calyx of H. sabdariffa L.) exhibited antioxidant activity and liver protection. Therefore, in this study, we explored the effect of HAs on human cancer cells. The result showed that HAs could cause cancer cell apoptosis, especially in HL-60 cells. Using flow cytometry, we found that HAs treatment (0-4 mg/ml) markedly induced apoptosis in HL-60 cells in a dose- and time-dependent manner. The result also revealed increased phosphorylation in p38 and c-Jun, cytochrome c release, and expression of tBid, Fas, and FasL in the HAs-treated HL-60 cells. We further used SB203580 (p38 inhibitor), PD98059 (MEK inhibitor), SP600125 (JNK inhibitor), and wortmannin (phosphatidylinositol 3-kinase; PI-3K inhibitor) to evaluate their effect on the HAs-induced HL-60 death. The data showed that only SB203580 had strong potential in inhibiting HL-60 cell apoptosis and related protein expression and phosphorylation. Therefore, we suggested that HAs mediated HL-60 apoptosis via the p38-FasL and Bid pathway. According to these results, HAs could be developed as chemopreventive agents. However, further investigations into the specificity and mechanism(s) of HAs are needed

  2. Plant growth retardation and conserved miRNAs are correlated to Hibiscus chlorotic ringspot virus infection.

    Gao, Ruimin; Wan, Zi Yi; Wong, Sek-Man

    2013-01-01

    Virus infection may cause a multiplicity of symptoms in their host including discoloration, distortion and growth retardation. Hibiscus chlorotic ringspot virus (HCRSV) infection was studied using kenaf (Hibiscus cannabinus L.), a non-wood fiber-producing crop in this study. Infection by HCRSV reduced the fiber yield and concomitant economic value of kenaf. We investigated kenaf growth retardation and fluctuations of four selected miRNAs after HCRSV infection. Vegetative growth (including plant height, leaf size and root development) was severely retarded. From the transverse and radial sections of the mock and HCRSV-infected kenaf stem, the vascular bundles of HCRSV-infected plants were severely disrupted. In addition, four conserved plant developmental and defence related microRNAs (miRNAs) (miR165, miR167, miR168 and miR171) and their respective target genes phabulosa (PHB), auxin response factor 8 (ARF8), argonaute 1 (AGO1) and scarecrow-like protein 1 (SCL1) displayed variation in expression levels after HCRSV infection. Compared with the mock inoculated kenaf plants, miR171 and miR168 and their targets SCL1 and AGO1 showed greater fluctuations after HCRSV infection. As HCRSV upregulates plant SO transcript in kenaf and upregulated AGO1 in HCRSV-infected plants, the expression level of AGO1 transcript was further investigated under sulfite oxidase (SO) overexpression or silencing condition. Interestingly, the four selected miRNAs were also up- or down-regulated upon overexpression or silencing of SO. Plant growth retardation and fluctuation of four conserved miRNAs are correlated to HCRSV infection.

  3. ROSELLE FLOWER (HIBISCUS SABDARIFFA IN THE TREATMENT OF HYPERTENSION IN POSTPARTUM MOTHERS

    Nikmah Jalilah Ritonga

    2017-07-01

    Full Text Available Background: Roselle flower (Hibiscus sabdariffa has been used as a food and herbal drinks, in hot and cold beverages, as a herbal medicine. Objective: To examine the effect of consuming roselle dried flower petals on changes in maternal hypertensive blood pressure during postpartum period. Methods: This was a Quasi experimental study with non-equivalent control group design conducted in 2016. There were 30 samples recruited by consecutive sampling with 15 samples in the intervention and control group. Data were analyzed using paired t-test to know the difference of blood pressure value before and after intervention in the intervention and control group, and Independent t-test to determine the difference of blood pressure decrease between intervention and control group. Mann-whitney and Wilcoxon test were also performed for non-normal data distribution. Results: Findings showed that there were statistically significant differences in systolic and diastolic blood pressure values before and after intervention in the intervention group with p-value 0.000 <0.05 with mean of systolic blood pressure decrease was 39.67 mmHg and diastolic was 22.33 mmHg. Additionally, there was a significant difference in the duration of healing between both groups with p-value 0.000 <0.05, with the average duration of healing in the intervention group was 2 days while in the control group was 3-4 days. Conclusion: There was significant effects of dried petals of rosella flowers (Hibiscus sabdariffa in lowering blood pressure, and there was significant differences in systolic and diastolic blood pressure and duration of healing between the intervention and control group of mothers who consumed antihypertensive drugs. The results of this study can be applied by midwives or health services in postpartum care with the cases of hypertension. It is expected that this intervention can help the healing process more quickly through a combination of antihypertensive medicines and

  4. Herbal products containing Hibiscus sabdariffa L., Crataegus spp., and Panax spp.: Labeling and safety concerns.

    Nunes, Maria Antónia; Rodrigues, Francisca; Alves, Rita C; Oliveira, Maria Beatriz P P

    2017-10-01

    Herbs have been used from ancient times for infusion preparation based on their potential health effects. In particular, the consumption of Hibiscus sabdariffa L., Crataegus spp. and Panax spp. has been largely associated to cardiovascular benefits. In this work, the label information of 52 herbal products for infusion preparation containing the referred herbs was analyzed and discussed, taking into consideration the European Union regulation for herbal products, which intends to protect public health and harmonize the legal framework in Member States. Details about the cardiovascular-related statements and warning notifications about consumption were considered. Also, regulatory issues and possible herb-drug interactions were explored and discussed. A total of 14 of the 52 herbal products selected presented health claims/statements on the label. Hibiscus was present in the majority of the products and, in some cases, it was mentioned only in the ingredients list and not on the product front-of-pack. Despite the promising outcomes of these plants to modulate cardiovascular risk markers, consumers with some sort of cardiovascular dysfunction and/or under medication treatments should be aware to carefully analyze the labels and consult additional information related to these herbal products. Manufacturers have also a huge responsibility to inform consumers by presenting awareness statements. Lastly, health professionals must advise and alert their patients about possible interactions that could occur between the concomitant consumption of drugs and herbs. Overall, there is still a real need of additional studies and clinical trials to better understand herbs effects and establish a science-based guidance to assess their safety. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Beneficial effects of Hibiscus rosa-sinensis L. flower aqueous extract in pregnant rats with diabetes

    Afiune, Luana Alves Freitas; Leal-Silva, Thaís; Sinzato, Yuri Karen; Moraes-Souza, Rafaianne Queiroz; Soares, Thaigra Sousa; Campos, Kleber Eduardo; Fujiwara, Ricardo Toshio; Herrera, Emilio; Damasceno, Débora Cristina

    2017-01-01

    Purpose The Hibiscus rosa-sinensis flower is widely used in Brazilian traditional medicine for the treatment of diabetes and has shown antifertility activity in female Wistar rats. However, there is no scientific confirmation of its effect on diabetes and pregnancy. The aim of this study was evaluate the effect of aqueous extract of H. rosa-sinensis flowers on maternal-fetal outcome in pregnant rats with diabetes. Methods Diabetes was induced by streptozotocin (STZ, 40 mg/kg) in virgin, adult, female Wistar rats. After diabetes induction, the rats were mated. The pregnant rats were distributed into four groups (n minimum = 11 animals/group): non-diabetic, non-diabetic treated, diabetic, and diabetic treated. Oral aqueous extract of Hibiscus rosa-sinensis was administered to rats in the treatment groups during pregnancy. At term pregnancy, maternal reproductive outcomes, fetal parameters, and biochemical parameters were analyzed. Results The non-diabetic treated group showed decreased high density lipoprotein cholesterol, increased atherogenic index (AI) and coronary artery risk index (CRI), and increased preimplantation loss rate compared to the non-diabetic group. Although treatment with H. rosa-sinensis led to no toxicity, it showed deleterious effects on cardiac and reproductive functions. However, the diabetic treated group showed increased maternal and fetal weights, reduced AI and CRI, and reduced preimplantation loss rate compared to the untreated diabetic group. Conclusion Our results demonstrate beneficial effects of this flower only in pregnant rats with diabetes and their offspring. Although these findings cannot be extrapolated to human clinical use, they show that the indiscriminate intake of H. rosa-sinensis may be harmful to healthy individuals and its use should be completely avoided in pregnancy. PMID:28644857

  6. ROSELLE FLOWER (HIBISCUS SABDARIFFA IN THE TREATMENT OF HYPERTENSION IN POSTPARTUM MOTHERS

    Nikmah Jalilah Ritonga

    2017-07-01

    Full Text Available Background: Roselle flower (Hibiscus sabdariffa has been used as a food and herbal drinks, in hot and cold beverages, as a herbal medicine. Objective: To examine the effect of consuming roselle dried flower petals on changes in maternal hypertensive blood pressure during postpartum period. Methods: This was a quasi-experimental study with non-equivalent control group design conducted in 2016. There were 30 samples recruited using consecutive sampling with 15 samples in the intervention group and control group. Data were analyzed using paired t-test to know the difference of blood pressure value before and after intervention in the intervention and control group, and Independent t-test to determine the difference of blood pressure decrease between intervention and control group. Mann-whitney and Wilcoxon test were also performed for data analysis. Results: Findings showed that there were statistically significant differences in systolic and diastolic blood pressure values before and after intervention in the intervention and control group with p-value 0.000 <0.05 with systolic blood pressure decrease was 5 mmHg and diastolic was 5.33 mmHg. Additionally, there was a significant effect in the duration of healing in the intervention group with p-value 0.000 <0.05, with the average duration of healing was 2 days. Conclusion: There was significant effects of dried petals of rosella flowers (Hibiscus sabdariffa brewed with 200 ml of hot water with temperature 90oC in lowering blood pressure, and there was significant differences in systolic and diastolic blood pressure and duration of healing between the intervention and control group of mothers who consumed antihypertensive drugs. The results of this study can be applied by midwives in postpartum care with the cases of hypertension. It is expected that this intervention can help the healing process more quickly through a combination of antihypertensive medicines and sedated petals of rosella flowers.

  7. Development and evaluation of Ketoprofen sustained release matrix tablet using Hibiscus rosa-sinensis leaves mucilage.

    Kaleemullah, M; Jiyauddin, K; Thiban, E; Rasha, S; Al-Dhalli, S; Budiasih, S; Gamal, O E; Fadli, A; Eddy, Y

    2017-07-01

    Currently, the use of natural gums and mucilage is of increasing importance in pharmaceutical formulations as valuable drug excipient. Natural plant-based materials are economic, free of side effects, biocompatible and biodegradable. Therefore, Ketoprofen matrix tablets were formulated by employing Hibiscus rosa-sinensis leaves mucilage as natural polymer and HPMC (K100M) as a synthetic polymer to sustain the drug release from matrix system. Direct compression method was used to develop sustained released matrix tablets. The formulated matrix tablets were evaluated in terms of physical appearance, weight variation, thickness, diameter, hardness, friability and in vitro drug release. The difference between the natural and synthetic polymers was investigated concurrently. Matrix tablets developed from each formulation passed all standard physical evaluation tests. The dissolution studies of formulated tablets revealed sustained drug release up to 24 h compared to the reference drug Apo Keto® SR tablets. The dissolution data later were fitted into kinetic models such as zero order equation, first order equation, Higuchi equation, Hixson Crowell equation and Korsmeyer-Peppas equation to study the release of drugs from each formulation. The best formulations were selected based on the similarity factor ( f 2 ) value of 50% and more. Through the research, it is found that by increasing the polymers concentration, the rate of drug release decreased for both natural and synthetic polymers. The best formulation was found to be F3 which contained 40% Hibiscus rosa-sinensis mucilage polymer and showed comparable dissolution profile to the reference drug with f 2 value of 78.03%. The release kinetics of this formulation has shown to follow non-Fickian type which involved both diffusion and erosion mechanism. Additionally, the statistical results indicated that there was no significant difference (p > 0.05) between the F3 and reference drug in terms of MDT and T50% with p

  8. Beneficial effects of Hibiscus rosa-sinensis L. flower aqueous extract in pregnant rats with diabetes.

    Luana Alves Freitas Afiune

    Full Text Available The Hibiscus rosa-sinensis flower is widely used in Brazilian traditional medicine for the treatment of diabetes and has shown antifertility activity in female Wistar rats. However, there is no scientific confirmation of its effect on diabetes and pregnancy. The aim of this study was evaluate the effect of aqueous extract of H. rosa-sinensis flowers on maternal-fetal outcome in pregnant rats with diabetes.Diabetes was induced by streptozotocin (STZ, 40 mg/kg in virgin, adult, female Wistar rats. After diabetes induction, the rats were mated. The pregnant rats were distributed into four groups (n minimum = 11 animals/group: non-diabetic, non-diabetic treated, diabetic, and diabetic treated. Oral aqueous extract of Hibiscus rosa-sinensis was administered to rats in the treatment groups during pregnancy. At term pregnancy, maternal reproductive outcomes, fetal parameters, and biochemical parameters were analyzed.The non-diabetic treated group showed decreased high density lipoprotein cholesterol, increased atherogenic index (AI and coronary artery risk index (CRI, and increased preimplantation loss rate compared to the non-diabetic group. Although treatment with H. rosa-sinensis led to no toxicity, it showed deleterious effects on cardiac and reproductive functions. However, the diabetic treated group showed increased maternal and fetal weights, reduced AI and CRI, and reduced preimplantation loss rate compared to the untreated diabetic group.Our results demonstrate beneficial effects of this flower only in pregnant rats with diabetes and their offspring. Although these findings cannot be extrapolated to human clinical use, they show that the indiscriminate intake of H. rosa-sinensis may be harmful to healthy individuals and its use should be completely avoided in pregnancy.

  9. Antioxidant and DNA damage protective properties of anthocyanin-rich extracts from Hibiscus and Ocimum: a comparative study.

    Sarkar, Biswatrish; Kumar, Dhananjay; Sasmal, Dinakar; Mukhopadhyay, Kunal

    2014-01-01

    Anthocyanin extracts (AEs) from Ocimum tenuiflorum (leaf), Hibiscus rosa-sinensis (petal) and Hibiscus sabdariffa (calyx) were investigated and compared for in vitro antioxidant activity and DNA damage protective property. Total phenolic content (TPC) and total anthocyanin content (TAC) of the AEs were determined and the major anthocyanins were characterised. In vitro antioxidant activities were assessed by ferric-reducing antioxidant power (FRAP) assay, 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical-scavenging activity, 2-deoxy-D-ribose degradation assay and lipid peroxidation assay. The protective property of the AEs was also examined against oxidative DNA damage by H2O2 and UV using pUC19 plasmid. All the AEs particularly those from O. tenuiflorum demonstrated efficient antioxidant activity and protected DNA from damage. Strong correlation between antioxidant capacity and TPC and TAC was observed. Significant correlation between antioxidant capacity and TPC and TAC ascertained that phenolics and anthocyanins were the major contributors of antioxidant activity.

  10. ffect of Nitrogen and Zinc Foliar Application on Quantitative Traits of Tea Rosslle (Hibiscus sabdariffa in Jiroft Zone

    abdolreza raisi sarbijan

    2017-02-01

    Full Text Available Introduction: Nitrogen is an essential element forplants and in combination withelements such as carbon, oxygen, hydrogen and sulfur results ineven more valuable materials such as amino acids, nucleic acids, alkaloids. Hibiscus tea (Hibiscus sabdariffa from Malvaceaefamily is known by different names in different parts of the world. In Iran it is calledthe Maki tea, tea Meccaorred tea.As an important plant,it is decided to investigate its growth and development in Jiroft. Materials and Methods The experiment was conducted as factorial based on randomized complete block design with three replications in farm research of Islamic Azad University of Jiroft during 2010. The first factor was nitrogen foliar application in four levels (0, 1, 2 and 3 percent and second factor was foliar application of zinc at twolevels (0 and 1 percent. The measured quantitative characteristics were stem diameter, plant height, calycle fresh weight,calycle dry weight, plant fresh weight,plant dry weight, leaf fresh weight,leaf dry weight, mucilage percentage and mucilage yield. Results and Discussion:The results of ANOVA showed that nitrogen foliar application on leaf dry weight, calycle fresh and dry weight was effective. Plant fresh weight, dry weight, stem diameter, plant height, mucilage percentageandmucilage yield showedsignificanteffects. Zinc foliar application significantly affected leaf fresh weight,leafdry weight, calycle fresh weight, plant fresh weight,plant dry weight, mucilage percentage andmucilage yield.The interaction effect of nitrogen and zinc on leaf dry weight, plant freshweight and plant dry weight was also significant. The mean comparison of studied characteristics revealed that byincreasing the amount of nitrogen up to N2 level, the stem diameter, plant height, leaf dry weight, calycle dry weight, mucilage percentage and yield increased but there was no significant difference between N2 and N3 levels. Plant fresh weight and plantdry weight

  11. Antimicrobial, antiparasitic and anticancer properties of Hibiscus sabdariffa (L.) and its phytochemicals: in vitro and in vivo studies.

    Hassan, Sherif T S; Berchová, Kateřina; Šudomová, Miroslava

    In the last few decades, Hibiscus sabdariffa L. (Malvaceae; H. sabdariffa) has gained much attention in research field because of its potentially useful bioactivity as well as a great safety and tolerability. For decades, microbial, parasitic and cancer diseases remain a serious threat to human health and animals as well. To treat such diseases, a search for new sources such as plants that provide various bioactive compounds useful in the treatment of several physiological conditions is urgently needed, since most of the drugs currently used in the therapy have several undesirable side effects, toxicity, and drug resistance. In this paper, we aim to present an updated overview of in vitro and in vivo studies that show the significant therapeutic properties of the crude extracts and phytochemicals derived from H. sabdariffa as antimicrobial, antiparasitic, and anticancer agents. The future directions of the use of H. sabdariffa in clinical trials will be discussed. Hibiscus sabdariffa L. antimicrobial agents cancer preventive agents antiparasitic drugs natural products.

  12. Anti-hepatotoxic activities of Hibiscus sabdariffa L. in animal model of streptozotocin diabetes-induced liver damage.

    Adeyemi, David O; Ukwenya, Victor O; Obuotor, Efere M; Adewole, Stephen O

    2014-07-30

    Flavonoid-rich aqueous fraction of methanolic extract of Hibiscus sabdariffa calyx was evaluated for its anti-hepatotoxic activities in streptozotocin-induced diabetic Wistar rats. Diabetes Mellitus was induced in Wistar rats by a single i.p injection of 80 mg/kg b.w. streptozotocin (STZ) dissolved in 0.1 M citrate buffer (pH 6.3). The ameliorative effects of the extract on STZ-diabetes induced liver damage was evident from the histopathological analysis and the biochemical parameters evaluated in the serum and liver homogenates. Reduced levels of glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) (3.76 ± 0.38 μM, 0.42 ± 0.04 U/L, 41.08 ± 3.04 U/ml, 0.82 ± 0.04 U/L respectively) in the liver of diabetic rats were restored to a near normal level in the Hibiscus sabdariffa-treated rats (6.87 ± 0.51 μM, 0.72 ± 0.06 U/L, 87.92 ± 5.26 U/ml, 1.37 ± 0.06 U/L respectively). Elevated levels of aspartate amino transferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP) in the serum of diabetic rats were also restored in Hibiscus sabdariffa -treated rats. Examination of stained liver sections revealed hepatic fibrosis and excessive glycogen deposition in the diabetic rats. These pathological changes were ameliorated in the extract-treated rats. The anti-hepatotoxic activity of Hibiscus sabdariffa extract in STZ diabetic rats could be partly related to its antioxidant activity and the presence of flavonnoids.

  13. Bioclimatic Thresholds, Thermal Constants and Survival of Mealybug, Phenacoccus solenopsis (Hemiptera: Pseudococcidae) in Response to Constant Temperatures on Hibiscus

    Sreedevi, Gudapati; Prasad, Yenumula Gerard; Prabhakar, Mathyam; Rao, Gubbala Ramachandra; Vennila, Sengottaiyan; Venkateswarlu, Bandi

    2013-01-01

    Temperature-driven development and survival rates of the mealybug, Phenacoccussolenopsis Tinsley (Hemiptera: Pseudococcidae) were examined at nine constant temperatures (15, 20, 25, 27, 30, 32, 35 and 40°C) on hibiscus ( Hibiscus rosa -sinensis L.). Crawlers successfully completed development to adult stage between 15 and 35°C, although their survival was affected at low temperatures. Two linear and four nonlinear models were fitted to describe developmental rates of P . solenopsis as a function of temperature, and for estimating thermal constants and bioclimatic thresholds (lower, optimum and upper temperature thresholds for development: Tmin, Topt and Tmax, respectively). Estimated thresholds between the two linear models were statistically similar. Ikemoto and Takai’s linear model permitted testing the equivalence of lower developmental thresholds for life stages of P . solenopsis reared on two hosts, hibiscus and cotton. Thermal constants required for completion of cumulative development of female and male nymphs and for the whole generation were significantly lower on hibiscus (222.2, 237.0, 308.6 degree-days, respectively) compared to cotton. Three nonlinear models performed better in describing the developmental rate for immature instars and cumulative life stages of female and male and for generation based on goodness-of-fit criteria. The simplified β type distribution function estimated Topt values closer to the observed maximum rates. Thermodynamic SSI model indicated no significant differences in the intrinsic optimum temperature estimates for different geographical populations of P . solenopsis . The estimated bioclimatic thresholds and the observed survival rates of P . solenopsis indicate the species to be high-temperature adaptive, and explained the field abundance of P . solenopsis on its host plants. PMID:24086597

  14. Volatiles and primary metabolites profiling in two Hibiscus sabdariffa (roselle) cultivars via headspace SPME-GC-MS and chemometrics.

    Farag, Mohamed A; Rasheed, Dalia M; Kamal, Islam M

    2015-12-01

    Hibiscus sabdariffa (roselle) is a plant of considerable commercial importance worldwide as functional food due to its organic acids, mucilage, anthocyanins, macro and micro-nutrients content. Although Hibiscus flowers are emerging as very competitive targets for phytochemical studies, very little is known about their volatile composition and or aroma, such knowledge can be suspected to be relevant for understanding its olfactory and taste properties. To provide insight into Hibiscus flower aroma composition and for its future use in food and or pharmaceutical industry, volatile constituents from 2 cultivars grown in Egypt, viz. Aswan and Sudan-1 were profiled using solid-phase microextraction (SPME) coupled to GCMS. A total of 104 volatiles were identified with sugar and fatty acid derived volatiles amounting for the major volatile classes. To reveal for cultivar effect on volatile composition in an untargeted manner, multivariate data analysis was applied. Orthogonal projection to latent structures-discriminant analysis (OPLS-DA) revealed for 1-octen-3-ol versus furfural/acetic acid enrichment in Aswan and Sudan-1 cvs., respectively. Primary metabolites contributing to roselle taste and nutritional value viz. sugars and organic acids were profiled using GC-MS after silylation. The impact of probiotic bacteria on roselle infusion aroma profile was further assessed and revealed for the increase in furfural production with Lactobacillus plantarum inoculation and without affecting its anthocyanin content. This study provides the most complete map for volatiles, sugars and organic acids distribution in two Hibiscus flower cultivars and its fermented product. Copyright © 2015 Elsevier Ltd. All rights reserved.

  15. The ant assemblage visiting extrafloral nectaries of Hibiscus pernambucensis (Malvaceae) in a mangrove forest in Southeast Brazil (Hymenoptera : Formicidae)

    Cogni, R; Freitas, AVL

    2002-01-01

    Ant species visiting extrafloral nectaries (EFNs) of Hibiscus pernambucensis were studied in a daily flooded mangrove forest in Picinguaba, Southeast Brazil. Nineteen ant species in five subfamilies were observed visiting the EFNs. The most common species (in order of abundance) were Camponotus sp.2, Brachymyrmex sp. and Pseudomyrmex gracilis during the warm season and Brachymyrmex sp., Camponotus crassus and Camponotus sp.2 during the cold season. A twenty-four hour census showed that ant ac...

  16. The impact of CO2 enrichment on fiber dimension and lignocellulose properties of three varieties of kenaf (Hibiscus cannabinus L.)

    Mahdi Khalatbari, A; Jaafar, H.Z.E; Ali Khalatbari, A

    2014-01-01

    The effects of two different carbon dioxide levels on fiber yield, fiber dimension and lignocelluloses properties of three varieties of kenaf (Hibiscus cannabinus L.) namely Fuhong (FH991), V36 and Kohn-Kaen60 (KK60) were assessed in a growth house experiment at faculty of Agriculture, Universiti Putra Malaysia. Seeds were sown in polyethylene bags containing top (loamy soil). Carbon dioxide enrichment treatment started when the seedlings reached four weeks and plants were exposed to 400 and ...

  17. CARACTERIZACIÓN QUÍMICA Y COLORIMÉTRICA DE CULTIVARES DE JAMAICA (Hibiscus sabdariffa L.)

    Camelo Méndez, Gustavo Adolfo

    2013-01-01

    La jamaica (Hibiscus sabdariffa L.) se destaca por su alto contenido en antocianinas y potencial actividad antioxidante. En la medicina tradicional se usa como: bactericida, diurético, cicatrizante y laxante entre otros. Actualmente, en México existen programas de mejoramiento genético tradicional de materiales, que han generado materiales de esta planta con diversos grados de pigmentación y tolerancia a plagas y enfermedades. El objetivo de este trabajo fue caracterizar químic...

  18. Effect of Tropical Rotation Crops on Meloidogyne incognita and Other Plant-Parasitic Nematodes.

    McSorley, R; Dickson, D W

    1995-12-01

    In a field experiment conducted on sandy soil in Florida during the 1993 season, rotation crops of castor (Ricinus communis), velvetbean (Mucuna deeringina), 'Mississippi Silver' cowpea (Vigna unguiculata), American jointvetch (Aeschynomene americana), 'Dehapine 51' cotton (Gossypium hirsutum), and 'SX-17' sorghum-sudangrass (Sorghum bicolor x S. sudanense) were effective in maintaining low population densities (450/100 cm(3) soil) resulted after 'Clemson Spineless' okra (Hibiscus esculentus) and 'Kirby' soybean (Glycine max). Following a winter cover crop of rye (Secale cereale), densities of M. incognita following the six most effective rotation crops (1993 season) remained relatively low (crop planted in 1994, but increased by the end of the eggplant crop. The rotation crops planted during 1993 had little effect on yield of eggplant in 1994. Eggplant yield was inversely correlated with preplant densities (Pi) of Belonolaimus longicaudatus (r = -0.282; P crop cultivars were lower (P crops intended for suppression of individual Meloidogyne spp. be evaluated for their response to other nematode pests as well.

  19. Tropical rotation crops influence nematode densities and vegetable yields.

    McSorley, R; Dickson, D W; de Brito, J A; Hochmuth, R C

    1994-09-01

    The effects of eight summer rotation crops on nematode densities and yields of subsequent spring vegetable crops were determined in field studies conducted in north Florida from 1991 to 1993. The crop sequence was as follows: (i) rotation crops during summer 1991; (ii) cover crop of rye (Secale cereale) during winter 1991-92; (iii) 'Lemondrop L' squash (Cucurbita pepo) during spring 1992; (iv) rotation crops during summer 1992; (v) rye during winter 1992-93; (vi) 'Classic' eggplant (Solanum melongena) during spring 1993. The eight summer crop rotation treatments were as follows: 'Hale' castor (Ricinus communis), velvetbean (Mucuna deeringiana), sesame (Sesamum indicum), American jointvetch (Aeschynomene americana), weed fallow, 'SX- 17' sorghum-sudangrass (Sorghum bicolor x S. sudanense), 'Kirby' soybean (Glycine max), and 'Clemson Spineless' okra (Hibiscus esculentus) as a control. Rotations with castor, velvetbean, American jointvetch, and sorghum-sudangrass were most effective in maintaining the lowest population densities of Meloidogyne spp. (a mixture of M. incognita race 1 and M. arenaria race 1), but Paratrichodorus minor built up in the sorghum-sudangrass rotation. Yield of squash was lower (P crops evaluated here may be useful for managing nematodes in the field and for improving yields of subsequent vegetable crops.

  20. Harvesting Microalgal Biomass grown in Anaerobic Sewage Treatment Effluent by the Coagulation-Flocculation Method: Effect of pH

    Servio Tulio Cassini

    2017-03-01

    Full Text Available ABSTRACT Harvesting is a critical step in microalgal biomass production process for many reasons. Among the existing techniques available for harvesting and dewatering microalgal biomass, recovery from aqueous medium by coagulation-flocculation has been the most economically viable process, althoughit is highly dependent on pH. This study aims to assess alternative coagulants compared to the standard coagulant aluminum sulfate for microalgal biomass recovery from anaerobic effluent of domestic sewage treatment. The effluent quality was also analyzed after biomass recovery. Coagulants represented by modified tannin, cationic starch and aluminum sulfate recovered more than 90% of algae biomass, at concentrations greater than 80 mg/L, in the pH range 7-10. Cationic starch promoted higher microalgal biomass recovery with a wider pH range. Powdered seeds of Moringa oleifera and Hibiscus esculentus(okra gum promoted biomass removal of 50%, only in the acidic range of pH. After sedimentation of the microalgal biomass, the effluents showed a removal of >80% for phosphorus and nitrogen values and >50% for BOD and COD when using aluminum sulfate, cationic starch and modified tannin as coagulants. Natural organic coagulants in a wide pH range can replace aluminum sulfate, a reference coagulant in microalgal biomass recovery, without decreasing microalgal biomass harvesting efficiency and the quality of the final effluent.

  1. Hibiscus rosa sinensis Linn. Petals Modulates Glycogen Metabolism and Glucose Homeostasis Signalling Pathway in Streptozotocin-Induced Experimental Diabetes.

    Pillai, Sneha S; Mini, S

    2016-03-01

    The prevalence of diabetes mellitus is becoming more and more serious and reaches epidemic proportions worldwide. Scientific research is constantly looking for new agents that could be used as dietary functional ingredients in the fight against diabetes. The objective of the present study was to evaluate the effect of ethyl acetate fraction of Hibiscus rosa sinensis Linn. petals on experimental diabetes at a dose of 25 mg/kg body weight and it was compared with standard anti-diabetic drug metformin. The elevated levels of serum glucose (398.56 ± 35.78) and glycated haemoglobin (12.89 ± 1.89) in diabetic rats were significantly decreased (156.89 ± 14.45 and 6.12 ± 0.49, respectively) by Hibiscus rosa sinensis petals (EHRS) administration. Hepatotoxicity marker enzyme levels in serum were normalized. The fraction supplementation restored the glycogen content by regulating the activities of glycogen metabolizing enzymes. It significantly modulated the expressions of marker genes involved in glucose homeostasis signalling pathway. Histopathological analysis of liver and pancreas supported our findings. The overall effect was comparable with metformin. Hence, our study reveals the role of hibiscus petals for alleviation of diabetes complications, thus it can be propagated as a nutraceutical agent.

  2. The acute impact of polyphenols from Hibiscus sabdariffa in metabolic homeostasis: an approach combining metabolomics and gene-expression analyses.

    Beltrán-Debón, Raúl; Rodríguez-Gallego, Esther; Fernández-Arroyo, Salvador; Senan-Campos, Oriol; Massucci, Francesco A; Hernández-Aguilera, Anna; Sales-Pardo, Marta; Guimerà, Roger; Camps, Jordi; Menendez, Javier A; Joven, Jorge

    2015-09-01

    We explored the acute multifunctional effects of polyphenols from Hibiscus sabdariffa in humans to assess possible consequences on the host's health. The expected dynamic response was studied using a combination of transcriptomics and metabolomics to integrate specific functional pathways through network-based methods and to generate hypotheses established by acute metabolic effects and/or modifications in the expression of relevant genes. Data were obtained from healthy male volunteers after 3 hours of ingestion of an aqueous Hibiscus sabdariffa extract. The data were compared with data obtained prior to the ingestion, and the overall findings suggest that these particular polyphenols had a simultaneous role in mitochondrial function, energy homeostasis and protection of the cardiovascular system. These findings suggest beneficial actions in inflammation, endothelial dysfunction, and oxidation, which are interrelated mechanisms. Among other effects, the activation of the heme oxygenase-biliverdin reductase axis, the systemic inhibition of the renin-angiotensin system, the inhibition of the angiotensin-converting enzyme, and several actions mirroring those of the peroxisome proliferator-activated receptor agonists further support this notion. We also found concordant findings in the serum of the participants, which include a decrease in cortisol levels and a significant increase in the active vasodilator metabolite of bradykinin (des-Arg(9)-bradykinin). Therefore, our data support the view that polyphenols from Hibiscus sabdariffa play a regulatory role in metabolic health and in the maintenance of blood pressure, thus implying a multi-faceted impact in metabolic and cardiovascular diseases.

  3. High antiviral effects of hibiscus tea extract on the H5 subtypes of low and highly pathogenic avian influenza viruses.

    Baatartsogt, Tugsbaatar; Bui, Vuong N; Trinh, Dai Q; Yamaguchi, Emi; Gronsang, Dulyatad; Thampaisarn, Rapeewan; Ogawa, Haruko; Imai, Kunitoshi

    2016-10-01

    Viral neuraminidase inhibitors are widely used as synthetic anti-influenza drugs for the prevention and treatment of influenza. However, drug-resistant influenza A virus variants, including H5N1 highly pathogenic avian influenza viruses (HPAIVs), have been reported. Therefore, the discovery of novel and effective antiviral agents is warranted. We screened the antiviral effects of 11 herbal tea extracts (hibiscus, black tea, tencha, rosehip tea, burdock tea, green tea, jasmine tea, ginger tea, lavender tea, rose tea and oak tea) against the H5N1 HPAIV in vitro. Among the tested extracts, only the hibiscus extract and its fractionated extract (frHibis) highly and rapidly reduced the titers of all H5 HPAIVs and low pathogenic AIVs (LPAIVs) used in the pre-treatment tests of Madin-Darby canine kidney (MDCK) cells that were inoculated with a mixture of the virus and the extract. Immunogold electron microscopy showed that anti-H5 monoclonal antibodies could not bind to the deformed H5 virus particles pretreated with frHibis. In post-treatment tests of MDCK cells cultured in the presence of frHibis after infection with H5N1 HPAIV, the frHibis inhibited viral replication and the expression of viral antigens and genes. Among the plants tested, hibiscus showed the most prominent antiviral effects against both H5 HPAIV and LPAIV.

  4. Effect of Hibiscus sabdariffa Calices on Dyslipidemia in Obese Adolescents: A Triple-masked Randomized Controlled Trial

    Sabzghabaee, Ali Mohammad; Ataei, Ehsan; Kelishadi, Roya; Ghannadi, Alireza; Soltani, Rasool; Badri, Shirinsadat; Shirani, Shahin

    2013-01-01

    Conflict of interest: none declared. Objective We aimed to evaluate the effects of Hibiscus sabdariffa (HS) calices on controlling dyslipidemia in obese adolescents. Methodology In this triple blind randomized placebo-controlled clinical trial which was registered in the Iranian registry for clinical trials (IRCT201109122306N2), 90 obese adolescents aged 12-18 years with documented dyslipidemia were randomly assigned in two groups of cases who received 2 grams of fine powdered calices of Hibiscus sabdariffa per day for one month and controls who received placebo powder with the same dietary and physical activity recommendations and duration of exposure. Full lipid profile and fasting blood sugar measured before and after the trial. Data were analyzed using multivariate general linear model. Findings Overall, 72 participants (mean age of 14.21±1.6, 35 boys) completed the trial. The two arms of the study (cases and controls) were not statistically different in terms of age, gender, weight, body mass index (BMI) and lipid profile before the trial. Serum total cholesterol, low density lipoprotein cholesterol and serum triglyceride showed a significant decrease in cases group but high density lipoprotein cholesterol level was not changed significantly. Conclusion It is concluded that Hibiscus sabdariffa calyces powder may have significant positive effects on lipid profile of adolescents which maybe attributed to its polyphenolic and antioxidant content. Further studies are needed on dose-response and formulation optimization. PMID:24082826

  5. Acclimation of croton and hibiscus seedlings in response to the application of indobultiric acid and humic acid for rooting

    Lílian Estrela Borges Baldotto

    2015-06-01

    Full Text Available The vegetative propagation of ornamental plants can be accelerated by applying plant growth regulators. Amongst them, the use of auxins, plant hormones with physiological effects on cell elongation and rooting have stood out. Alternatively, the application of humic acids, bioactive fraction of soil organic matter, also results in increases in rooting cuttings of ornamental plants. The objective of this work was to study the growth characteristics and the nutritional contents of croton and hibiscus plants during acclimation of seedlings in response to different concentrations of indolebutyric acid (IBA and humic acid (HA applied to cuttings for rooting. The experiment was conducted in greenhouse, and the apical stem cuttings were treated with solutions with concentrations of 0, 250, 500, 1000 and 2000 mg L-1of IBA and 0, 10, 20, 30 and 40 mg L-1 of C from HA. At 45 days of rooting in carbonized rice husk, they were individually transferred to plastic bags of 2.0 dm3 containing a mixture of soil: sand: manure (2: 1: 1 as substrate. At 90 days of acclimation, the plants were collected for measurement of growth and nutritional variables. The results showed that the application of the IBA stimulates the absorption of nutrients and growth of croton cuttings and transplanted hibiscus, contributing to formation of vigorous seedlings. A similar response occurred with the application of HA in hibiscus cuttings

  6. Biology of Maconellicoccus hirsutus (Hemiptera: Pseudococcidae on Hibiscus rosa-sinensis = Biologia de Maconellicoccus hirsutus (Hemiptera: Pseudococcidae em Hibiscus rosa-sinensis

    Marcelo Negrini

    2017-10-01

    Full Text Available Maconellicoccus hirsutus Green, 1908 (Hemiptera: Pseudococcidae is an introduced pest that threatens fruit and ornamental plant production in Brazil. Reported in Brazil for the first time in 2010, in the state of Roraima, M. hirsutus, has spread rapidly to other regions of the country. The objective of this study was to investigate the biology of M. hirsutus on Hibiscus rosa-sinensis L. (Malvaceae leaves in laboratory conditions with climatic parameters similar to those of the Brazilian North and Northeast (27 ± 1 °C, 60 ± 10% relative humidity and 12 hours light and 12 hours dark. Eighty M. hirsutus nymphs were individualized on rooted H. rosa-sinensis leaves and their survival, mortality, sex ratio, and egg numbers per female emerged were recorded. Maconellicoccus hirsutus can have up to nine generations per year, taking 6.5 days to double its population size. The female numbers of this pest are about three times higher than those for its male counterparts, with 98 eggs per female and 97% viability. The survival curve of the species is type I, that is, mortality rates are higher in adulthood. Maconellicoccus hirsutus, especially, a pest of fruit trees and ornamental plants, presents great potential for population growth in Brazilian tropical conditions. = Maconellicoccus hirsutus Green, 1908 (Hemiptera: Pseudococcidae é uma praga introduzida que ameaça a produção de frutas e plantas ornamentais no Brasil. Registrada no Brasil pela primeira vez em 2010, no estado de Roraima, M. hirsutus se espalhou rapidamente para outras regiões do país. O objetivo deste estudo foi investigar a biologia de M. hirsutus em folhas de Hibiscus rosa-sinensis L. (Malvaceae, em condições de laboratório com parâmetros climáticos semelhantes aos das regiões Norte e Nordeste do Brasil (27 ± 1 °C, 60 ± 10 % de umidade relativa e 12 h de luz e 12 h de escuro. Oitenta ninfas de M. hirsutus foram individualizadas em folhas enraizadas de H. rosa-sinensis e

  7. Hibiscus cannabinus

    morufat

    2013-05-08

    May 8, 2013 ... of kenaf raw materials is scarce, with Africa producing only 2.91% of the global ... Skoog (MS), 1962) medium containing (per litre) 30 g sucrose, 0.1 g myo-inositol and 7 g ..... Muchow RC, Wood LM. (1983). Effect of sowing ...

  8. Hibiscus cannabinus

    morufat

    2013-05-08

    May 8, 2013 ... myo-inositol, 0.1 mg 2, 4-dichlorophenoxy acetic acid (2, 4- D), 0.5 mg kinetin and 8 g agar per litre of ... acetic acid (NAA), 0.3 mg 6-benzyl amino-purine (BAP) and 8 g agar set at pH 5.7 and incubated at 12 h ... recorded in V400, suggesting its compatibility with equa- torial climates for optimum fibre yields.

  9. Hibiscus sabdariffa

    physicochemical properties and the FTIR spectra over the storage period revealed different rates and fashions of deterioration. ... and it's majorly used in brewing (Adejumo,. 2003). .... phenolic compounds (Kumburawa et al.,. 2007). ... The microbial content was analyzed .... well-known for antioxidant, antimicrobial and.

  10. African Journal of Biotechnology - Vol 10, No 54 (2011)

    Morphological classification of genetic diversity in cultivated okra, Abelmoschus esculentus (L) Moench using principal component analysis (PCA) and single ... Evaluation of 14 winter bread wheat genotypes in normal irrigation and stress conditions after anthesis stage · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT

  11. Author Details

    Nwaoguala, CNC. Vol 15, No 2 (2015) - Articles Influence by artificial defoliation and NPK fertilizer application on growth and yield of Okra (Abelmoschus esculentus (L) Moench) Abstract PDF. ISSN: 1684-5374. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More ...

  12. Genetic Diversity of Hibiscus tiliaceus (Malvaceae) in China Assessed using AFLP Markers

    TANG, TIAN; ZHONG, YANG; JIAN, SHUGUANG; SHI, SUHUA

    2003-01-01

    Amplified fragment length polymorphism (AFLP) markers were used to investigate the genetic variations within and among nine natural populations of Hibiscus tiliaceus in China. DNA from 145 individuals was amplified with eight primer pairs. No polymorphisms were found among the 20 samples of a marginal population of recent origin probably due to a founder effect. Across the other 125 individuals, 501 of 566 bands (88·5 %) were polymorphic, and 125 unique AFLP phenotypes were observed. Estimates of genetic diversity agreed with life history traits of H. tiliaceus and geographical distribution. AMOVA analysis revealed that most genetic diversity resided within populations (84·8 %), which corresponded to results reported for outcrossing plants. The indirect estimate of gene flow based on ϕST was moderate (Nm = 1·395). Long-distance dispersal of floating seeds and local environments may play an important role in shaping the genetic diversity of the population and the genetic structure of this species. PMID:12930729

  13. Preliminary X-ray Data Analysis of Crystalline Hibiscus Chlorotic Ringspot Virus

    Cheng, A.; Speir, J; Yuan, Y; Johnson, J; Wong, S

    2009-01-01

    Hibiscus chlorotic ringspot virus (HCRSV) is a positive-sense monopartite single-stranded RNA virus that belongs to the Carmovirus genus of the Tombusviridae family, which includes carnation mottle virus (CarMV). The HCRSV virion has a 30 nm diameter icosahedral capsid with T = 3 quasi-symmetry containing 180 copies of a 38 kDa coat protein (CP) and encapsidates a full-length 3.9 kb genomic RNA. Authentic virus was harvested from infected host kenaf leaves and was purified by saturated ammonium sulfate precipitation, sucrose density-gradient centrifugation and anion-exchange chromatography. Virus crystals were grown in multiple conditions; one of the crystals diffracted to 3Synchrotron .2 A resolution and allowed the collection of a partial data set. The crystal belonged to space group R32, with unit-cell parameters a = b = 336.4, c = 798.5 . Packing considerations and rotation-function analysis determined that there were three particles per unit cell, all of which have the same orientation and fixed positions, and resulted in tenfold noncrystallography symmetry for real-space averaging. The crystals used for the structure determination of southern bean mosaic virus (SBMV) have nearly identical characteristics. Together, these findings will greatly aid the high-resolution structure determination of HCRSV.

  14. Antimutagenic Effect of Hibiscus sabdariffa L. Aqueous Extract on Rats Treated with Monosodium Glutamate.

    Gheller, Ana Carla Guidini Valentini; Kerkhoff, Jacqueline; Vieira Júnior, Gerardo Magela; de Campos, Kleber Eduardo; Sugui, Marina Mariko

    2017-01-01

    Hibiscus sabdariffa L. is a plant of the Malvaceae family, commonly known as roselle. H. sabdariffa is known to contain antioxidant, cholesterol-lowering, antiobesity, insulin resistance reduction, antihypertensive, and skin cancer chemopreventive properties. This study evaluated the effects of H. sabdariffa aqueous extract against cyclophosphamide (CPA, 25 mg/Kg) induced damage to DNA in male Wistar rats by micronucleus test. Samples of H. sabdariffa calyx were obtained in the municipality of Barra do Garças, Mato Grosso, Brazil. The aqueous extract was prepared by infusion and each animal received a daily dose of 400 mg/Kg by gavage for 15 consecutive days of treatment. The presence of anthocyanins was confirmed by ferric chloride test and phenolic compounds using high-performance liquid chromatography, with emphasis on the identification of rutin. The animals were sacrificed by deepening of anaesthesia to obtain bone marrow and determination of the frequency of micronucleated polychromatic erythrocytes. The group treated with the aqueous extract of H. sabdariffa revealed a 91% reduction in micronucleus frequency when compared with the positive control group. Under the conditions tested, H. sabdariffa L. presented a protective effect to CPA-induced damage to DNA of the treated animals, and it is a potential candidate as a chemopreventive agent against carcinogenesis.

  15. Genome analysis of Hibiscus syriacus provides insights of polyploidization and indeterminate flowering in woody plants.

    Kim, Yong-Min; Kim, Seungill; Koo, Namjin; Shin, Ah-Young; Yeom, Seon-In; Seo, Eunyoung; Park, Seong-Jin; Kang, Won-Hee; Kim, Myung-Shin; Park, Jieun; Jang, Insu; Kim, Pan-Gyu; Byeon, Iksu; Kim, Min-Seo; Choi, JinHyuk; Ko, Gunhwan; Hwang, JiHye; Yang, Tae-Jin; Choi, Sang-Bong; Lee, Je Min; Lim, Ki-Byung; Lee, Jungho; Choi, Ik-Young; Park, Beom-Seok; Kwon, Suk-Yoon; Choi, Doil; Kim, Ryan W

    2017-02-01

    Hibiscus syriacus (L.) (rose of Sharon) is one of the most widespread garden shrubs in the world. We report a draft of the H. syriacus genome comprised of a 1.75 Gb assembly that covers 92% of the genome with only 1.7% (33 Mb) gap sequences. Predicted gene modeling detected 87,603 genes, mostly supported by deep RNA sequencing data. To define gene family distribution among relatives of H. syriacus, orthologous gene sets containing 164,660 genes in 21,472 clusters were identified by OrthoMCL analysis of five plant species, including H. syriacus, Arabidopsis thaliana, Gossypium raimondii, Theobroma cacao and Amborella trichopoda. We inferred their evolutionary relationships based on divergence times among Malvaceae plant genes and found that gene families involved in flowering regulation and disease resistance were more highly divergent and expanded in H. syriacus than in its close relatives, G. raimondii (DD) and T. cacao. Clustered gene families and gene collinearity analysis revealed that two recent rounds of whole-genome duplication were followed by diploidization of the H. syriacus genome after speciation. Copy number variation and phylogenetic divergence indicates that WGDs and subsequent diploidization led to unequal duplication and deletion of flowering-related genes in H. syriacus and may affect its unique floral morphology. © The Author 2016. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

  16. Accumulation of Kaempferitrin and Expression of Phenyl-Propanoid Biosynthetic Genes in Kenaf (Hibiscus cannabinus

    Shicheng Zhao

    2014-10-01

    Full Text Available Kenaf (Hibiscus cannabinus is cultivated worldwide for its fiber; however, the medicinal properties of this plant are currently attracting increasing attention. In this study, we investigated the expression levels of genes involved in the biosynthesis of kaempferitrin, a compound with many biological functions, in different kenaf organs. We found that phenylalanine ammonia lyase (HcPAL was more highly expressed in stems than in other organs. Expression levels of cinnamate 4-hydroxylase (HcC4H and 4-coumarate-CoA ligase (Hc4CL were highest in mature leaves, followed by stems and young leaves, and lowest in roots and mature flowers. The expression of chalcone synthase (HcCHS, chalcone isomerase (HcCHI, and flavone 3-hydroxylase (HcF3H was highest in young flowers, whereas that of flavone synthase (HcFLS was highest in leaves. An analysis of kaempferitrin accumulation in the different organs of kenaf revealed that the accumulation of this compound was considerably higher (>10-fold in leaves than in other organs. On the basis of a comparison of kaempferitrin contents with the expression levels of different genes in different organs, we speculate that HcFLS plays an important regulatory role in the kaempferitrin biosynthetic pathway in kenaf.

  17. Anti-inflammatory activities of essential oil isolated from the calyx of Hibiscus sabdariffa L.

    Shen, Chun-Yan; Zhang, Tian-Tian; Zhang, Wen-Li; Jiang, Jian-Guo

    2016-10-12

    Hibiscus sabdariffa Linn., belonging to the family of Malvaceae, is considered to be a plant with health care applications in China. The main purpose of this study was to analyze the composition of its essential oil and assess its potential therapeutic effect on anti-inflammatory activity. A water steam distillation method was used to extract the essential oil from H. Sabdariffa. The essential oil components were determined by gas chromatography/mass spectrometry (GC-MS) analysis and a total of 18 volatile constituents were identified, the majority of which were fatty acids and ester compounds. Biological activity showed that the essential oil extracted from H. Sabdariffa exhibited excellent anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated macrophage RAW 264.7 cells. The nitric oxide (NO) inhibition rate reached 67.46% when the concentration of the essential oil was 200 μg mL -1 . Further analysis showed that the anti-inflammatory activity of the essential oil extracted from H. Sabdariffa might be exerted through inhibiting the activation of NF-κB and MAPK (JNK and ERK1/2) signaling pathways to decrease NO and pro-inflammatory cytokine (IL-1, IL-6, TNF-α, COX-2, and iNOS) production. Thus, the essential oil extracted from H. Sabdariffa is a good source of a natural product with a beneficial effect against inflammation, and it may be applied as a food supplement and/or functional ingredient.

  18. Ultrasonic-assisted extraction and in-vitro antioxidant activity of polysaccharide from Hibiscus leaf.

    Afshari, Kasra; Samavati, Vahid; Shahidi, Seyed-Ahmad

    2015-03-01

    The effects of ultrasonic power, extraction time, extraction temperature, and the water-to-raw material ratio on extraction yield of crude polysaccharide from the leaf of Hibiscus rosa-sinensis (HRLP) were optimized by statistical analysis using response surface methodology. The response surface methodology (RSM) was used to optimize HRLP extraction yield by implementing the Box-Behnken design (BBD). The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and also analyzed by appropriate statistical methods (ANOVA). Analysis of the results showed that the linear and quadratic terms of these four variables had significant effects. The optimal conditions for the highest extraction yield of HRLP were: ultrasonic power, 93.59 W; extraction time, 25.71 min; extraction temperature, 93.18°C; and the water to raw material ratio, 24.3 mL/g. Under these conditions, the experimental yield was 9.66±0.18%, which is well in close agreement with the value predicted by the model 9.526%. The results demonstrated that HRLP had strong scavenging activities in vitro on DPPH and hydroxyl radicals. Copyright © 2014 Elsevier B.V. All rights reserved.

  19. Accumulation of kaempferitrin and expression of phenyl-propanoid biosynthetic genes in kenaf (Hibiscus cannabinus).

    Zhao, Shicheng; Li, Xiaohua; Cho, Dong Ha; Arasu, Mariadhas Valan; Al-Dhabi, Naif Abdullah; Park, Sang Un

    2014-10-23

    Kenaf (Hibiscus cannabinus) is cultivated worldwide for its fiber; however, the medicinal properties of this plant are currently attracting increasing attention. In this study, we investigated the expression levels of genes involved in the biosynthesis of kaempferitrin, a compound with many biological functions, in different kenaf organs. We found that phenylalanine ammonia lyase (HcPAL) was more highly expressed in stems than in other organs. Expression levels of cinnamate 4-hydroxylase (HcC4H) and 4-coumarate-CoA ligase (Hc4CL) were highest in mature leaves, followed by stems and young leaves, and lowest in roots and mature flowers. The expression of chalcone synthase (HcCHS), chalcone isomerase (HcCHI), and flavone 3-hydroxylase (HcF3H) was highest in young flowers, whereas that of flavone synthase (HcFLS) was highest in leaves. An analysis of kaempferitrin accumulation in the different organs of kenaf revealed that the accumulation of this compound was considerably higher (>10-fold) in leaves than in other organs. On the basis of a comparison of kaempferitrin contents with the expression levels of different genes in different organs, we speculate that HcFLS plays an important regulatory role in the kaempferitrin biosynthetic pathway in kenaf.

  20. Hibiscus rosa- sinensis leaf extract as coagulant aid in leachate treatment

    Awang, Nik Azimatolakma; Aziz, Hamidi Abdul

    2012-12-01

    Hibiscus rosa- sinensis is a biodegradable material that has remained untested for flocculating properties. The objective of this study is to examine the efficiency of coagulation-flocculation processes for the removal of color, iron (Fe3+), suspended solids, turbidity and ammonia nitrogen(NH3-N), from landfill leachate using 4,000 mg/L alum in conjunction with H. rosa- sinensis leaf extract (HBaqs). Hydroxyl (O-H) and (carboxyl) C=O functional groups along the HBaqs chain help to indulge flocculating efficiency of HBaqs via bridging. The experiments confirm the positive coagulation properties of HBaqs. The Fe3+ removal rate using 4,000 mg/L alum as sole coagulant was approximately 60 %, and increased to 100 % when 4,000 mg/L alum was mixed with 500 mg/L HBaqs. By mixing, 4,000 mg/L alum with 100-500 mg/L HBaqs, 72 % of SS was removed as compared with only 45 % reduction using 4,000 mg/L alum as sole coagulant.

  1. PENGARUH PEMBERIAN SUBKRONIK EKSTRAK KELOPAK BUNGA ROSELLA (Hibiscus Sabdariffa L. TERHADAP KADAR SGPT SGOT DAN ALP

    Nurkhasanah Nurkhasanah

    2016-11-01

    Full Text Available Roselle (Hibiscus sabdariffa L. has been known as traditional medicinal plants. It needed to evaluate the safety of roselle extract on long-term oral administration. The research aimed to determine the savety of ethanolic extract of roselle calyx. Subchronic toxicity study of ethanolic extract of roselle calyx had been carried out on 60 Sprague Dawleys (SD rats for 28 days. The roselle extract was administered orally every day, with doses of 50,100 and 200 mg/kgBW. There are 2 satellite groups, kept for another 14 days after the treatment in order to detect a delayed occurrence of toxic effect. Satellite group was given extract 200 mg/kgBW for 28 days and followed aquadest treatment for 14 days. At the end of experiment the blood was collected for meansuring SGPT, SGOT and ALP activity. The result showed that treatment of roselle calyx extract had no significant changes in SGPT SGOT, ALP activity. The delayed effect was not also observed. The ethanolic extract of roselle calyx didn’t show toxic effects on the liver on subchronic administration.

  2. Infusion of Hibiscus sabdariffa L. Modulates Oxidative Stress in Patients with Marfan Syndrome

    Soto, María Elena; Zuñiga-Muñoz, Alejandra; Guarner Lans, Verónica; Duran-Hernández, Erendira Janet; Pérez-Torres, Israel

    2016-01-01

    Marfan syndrome (MFS) is associated with progressive aortic dilatation, endothelial dysfunction, and oxidative stress that contribute to the early acute dissection of the vessel and can end up in rupture of the aorta and sudden death. Many studies have described that the organic acids from Hibiscus sabdariffa Linne (HSL) calyces increase cellular antioxidant capacity and decrease oxidative stress. Here we evaluate if the antioxidant properties of HSL infusion improve oxidative stress in MFS patients. Activities of extra cellular super oxide dismutase (ECSOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), glutathione reductase (GSSG-R), glutathione (GSH), lipid peroxidation (LPO) index, total antioxidant capacity (TAC), and ascorbic acid were determined in plasma from MFS patients. Values before and after 3 months of the treatment with 2% HSL infusion were compared in control and MFS subjects. After treatment, there was a significant decrease in ECSOD (p = 0.03), EGPx (p = 0.04), GST (p = 0.03), GSH (p = 0.01), and TAC and ascorbic acid (p = 0.02) but GSSG-R activity (p = 0.04) and LPO (p = 0.02) were increased in MFS patients in comparison to patients receiving the HSL treatment and C subjects. Therefore, the infusion of HSL calyces has antioxidant properties that allow an increase in antioxidant capacity of both the enzymatic and nonenzymatic systems, in the plasma of the MSF patients. PMID:27413258

  3. Phenolic compounds of Hibiscus sabdariffa and influence of organic residues on its antioxidant and antitumoral properties

    ASN. Formagio

    Full Text Available The aim of this study was to evaluate the phenolic and flavonoids contents and the antioxidant and antitumoral activity of leaf and calyx methanolic extracts from Hibiscus sabdariffa (roselle cultivated with poultry litter and organosuper® under three modes of application. The total phenolic content in the each extract was determined using the Folin-Ciocalteu reagent and for aluminium chloride flavonoids. The antioxidant parameters were analyzed using a 2, 2-diphenyl-1-picrylhydrazyl (DPPH. free radical scavenging assay. An antitumor colorimetric assay using sulforhodamine B. The highest contents of phenolic and flavonoids were observed in leaf extracts (389.98 and 104.52 mg g–1, respectively and calyx extracts (474.09 and 148.35 mg g–1, respectively from plants cultivated with organosuper®, although these values did not differ significantly from those observed for the other treatments. The average IC50 of leaves (43.48 μg mL–1 and calyces (37.15 μg mL–1 demonstrated that both have substances that may contribute to free radical scavenging action. The methanol extract from calyces showed significant selective activity against a leukemia line (K-562, with IC50 values of 0.12 mg mL–1 (organosuper® and 1.16 mg mL–1 (poultry litter, with concentration-dependent, cytotoxic and cytocidal effects.

  4. Hibiscus chlorotic ringspot virus coat protein upregulates sulfur metabolism genes for enhanced pathogen defense.

    Gao, Ruimin; Ng, Florence Kai Lin; Liu, Peng; Wong, Sek-Man

    2012-12-01

    In both Hibiscus chlorotic ringspot virus (HCRSV)-infected and HCRSV coat protein (CP) agroinfiltrated plant leaves, we showed that sulfur metabolism pathway related genes-namely, sulfite oxidase (SO), sulfite reductase, and adenosine 5'-phosphosulfate kinase-were upregulated. It led us to examine a plausible relationship between sulfur-enhanced resistance (SED) and HCRSV infection. We broadened an established method to include different concentrations of sulfur (0S, 1S, 2S, and 3S) to correlate them to symptom development of HCRSV-infected plants. We treated plants with glutathione and its inhibitor to verify the SED effect. Disease resistance was induced through elevated glutathione contents during HCRSV infection. The upregulation of SO was related to suppression of symptom development induced by sulfur treatment. In this study, we established that HCRSV-CP interacts with SO which, in turn, triggers SED and leads to enhanced plant resistance. Thus, we have discovered a new function of SO in the SED pathway. This is the first report to demonstrate that the interaction of a viral protein and host protein trigger SED in plants. It will be interesting if such interaction applies generally to other host-pathogen interactions that will lead to enhanced pathogen defense.

  5. Phytochemistry, antioxidant capacity, total phenolic content and anti-inflammatory activity of Hibiscus sabdariffa leaves.

    Zhen, Jing; Villani, Thomas S; Guo, Yue; Qi, Yadong; Chin, Kit; Pan, Min-Hsiung; Ho, Chi-Tang; Simon, James E; Wu, Qingli

    2016-01-01

    A liquid chromatography-mass spectrometry method was developed for the simultaneous separation, and determination of natural compounds including phenolic acids and flavonoids in the leaves of Hibiscus sabdariffa. By analyzing the UV and MS data, and comparison with authenticated standards, 10 polyphenols including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, quercetin, kaempferol and their glycosides were identified together with 5-(hydroxymethyl)furfural. Major constituents in the leaves of 25 different populations from worldwide accessions were quantified and compared with each other. The total phenolic content of each accession was determined using Folin-Ciocalteu assay, ranging from 18.98 ± 2.7 to 29.9 ± 0.5 mg GAE/g. Their in vitro antioxidant activities were measured by ABTS radical cation decolorization assay, varying from 17.5 to 152.5 ± 18.8 μmol Trolox/g. After the treatment of H. sabdariffa leaf extract, the reduction of LPS-induced NO production dose-dependently in RAW 264.7 cell indicates the extract's potential anti-inflammatory activity. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. Hemidesmus indicus and Hibiscus rosa-sinensis Affect Ischemia Reperfusion Injury in Isolated Rat Hearts

    Vinoth Kumar Megraj Khandelwal

    2011-01-01

    Full Text Available Hemidesmus indicus (L. R. Br. (HI and Hibiscus rosa-sinensis L. (HRS are widely used traditional medicine. We investigated cardioprotective effects of these plants applied for 15 min at concentrations of 90, 180, and 360 μg/mL in Langendorff-perfused rat hearts prior to 25-min global ischemia/120-min reperfusion (I/R. Functional recovery (left ventricular developed pressure—LVDP, and rate of development of pressure, reperfusion arrhythmias, and infarct size (TTC staining served as the endpoints. A transient increase in LVDP (32%–75% occurred at all concentrations of HI, while coronary flow (CF was significantly increased after HI 180 and 360. Only a moderate increase in LVDP (21% and 55% and a tendency to increase CF was observed at HRS 180 and 360. HI and HRS at 180 and 360 significantly improved postischemic recovery of LVDP. Both the drugs dose-dependently reduced the numbers of ectopic beats and duration of ventricular tachycardia. The size of infarction was significantly decreased by HI 360, while HRS significantly reduced the infarct size at all concentrations in a dose-dependent manner. Thus, it can be concluded that HI might cause vasodilation, positive inotropic effect, and cardioprotection, while HRS might cause these effects at higher concentrations. However, further study is needed to elucidate the exact mechanism of their actions.

  7. Preharvest, harvest and postharvest factors inherent to roselle (Hibiscus sabdariffa L. crop: A review.

    Balois-Morales, R.

    2016-01-01

    Full Text Available Hibiscus sabdariffa L. is cultivated to obtain fiber from the stems, oil from the seeds, and to harvest its calyces. Currently, there is a growing interest for the calyces due to studies that have proven their functional feature as the antioxidant effect, alluded to its anthocyanin content, and other benefits that expand their use to the pharmaceutical sector. However, there are no studies focused on relating all the factors involved in the process, from sowing and preharvest management, to harvest, postharvest management, distribution and the sale of dried calyces. The aim of this review is to highlight the factors involved in Roselle management such as variety, crop nutrition, sowing date, irrigation, plagues and diseases incidence and use of phytohormones. To establish the optimal time of calyces harvest, studies have been focused on determining aspects such as size, pH, dry matter content and anthocyanins at different times after blooming. As for the postharvest, the trend marked by studies is to implement improvements in the drying process of calyces and to let producers, distributors and sellers know the quality standards because much of the damage during postharvest is associated with deficiencies in the way calyces are handled.

  8. Germination biology of Hibiscus tridactylites in Australia and the implications for weed management

    Chauhan, Bhagirath Singh

    2016-05-01

    Hibiscus tridactylites is a problematic broadleaf weed in many crops in Australia; however, very limited information is available on seed germination biology of Australian populations. Experiments were conducted to evaluate the effect of environmental factors on germination and emergence of H. tridactylites. Germination was stimulated by seed scarification, suggesting the inhibition of germination in this species is mainly due to the hard seed coat. Germination was not affected by light conditions, suggesting that seeds of this species are not photoblastic. Germination was higher at alternating day/night temperatures of 30/20 °C (74%) and 35/25 °C (69%) than at 25/15 °C (63%). Moderate salinity and water stress did not inhibit germination of H. tridactylites. Seedling emergence of H. tridactylites was highest (57%) for the seeds buried at a 2 cm depth in the soil; 18% of seedlings emerged from seeds buried at 8 cm but no seedlings emerged below this depth. Soil inversion by tillage to bury weed seeds below their maximum depth of emergence could serve an important tool for managing H. tridactylites.

  9. Changes in protein patterns and in vivo protein synthesis during senescence of hibiscus petals

    Woodson, W.R.; Handa, A.K.

    1986-01-01

    Changes in proteins associated with senescence of the flowers of Hibiscus rosa-sinensis was studied using SDS-PAGE. Total extractable protein from petals decreased with senescence. Changes were noted in patterns of proteins from aging petals. Flower opening and senescence was associated with appearance and disappearance of several polypeptides. One new polypeptide with an apparent mw of 41 kd was first seen the day of flower opening and increased to over 9% of the total protein content of senescent petal tissue. Protein synthesis during aging was investigated by following uptake and incorporation of 3 H-leucine into TCA-insoluble fraction of petal discs. Protein synthesis, as evidenced by the percent of label incorporated into the TCA-insoluble fraction, was greatest (32%) the day before flower opening. Senescent petal tissue incorporated 4% of label taken up into protein. Proteins were separated by SDS-PAGE and labelled polypeptides identified by fluorography. In presenescent petal tissue, radioactivity was distributed among several major polypeptides. In senescent tissue, much of the radioactivity was concentrated in the 41 kd polypeptide

  10. Antibacterial activity of Hibiscus sabdariffa L. calyces against hospital isolates of multidrug resistant Acinetobacter baumannii

    Emad Mohamed Abdallah

    2016-11-01

    Full Text Available Objective: To evaluate the antibacterial activity of methanol extract of Hibiscus sabdariffa (H. sabdariffa calyces employed in Sudanese folk medicine against five hospital isolates of multidrug resistant Acinetobacter baumannii (MDR A. baumannii. Methods: The antibacterial activity of 80% methanol extract (v/v of H. sabdariffa calyces was evaluated by agar disc diffusion, minimum inhibitory concentration and minimum bactericidal concentration methods. Antibiotic susceptibility of selected A. baumannii strains was tested. Results: In the present investigation, the methanol extract from the calyces of H. sabdariffa exhibited significant antibacterial properties against the non-MDR A. baumannii as well as the MDR A. baumannii strains with a zone of inhibition ranging from (11.3 ± 0.3 to (13.6 ± 0.3 mm. The relative percentage inhibition of H. sabdariffa extract (10 mg/disc with respect to gentamicin (10 mg/disc had potent antibacterial properties and was much more effective than gentamicin. Values of minimum inhibitory concentration and minimum bactericidal concentration ranged from 25 to 50 and 50 to 100 mg/mL, respectively, revealing the potential bactericidal properties of the extract. Conclusions: According to the present study, the calyces of H. sabdariffa can be used as a substitute source of the current ineffective synthetic antibiotics used against MDR A. baumannii.

  11. Green synthesis of gold and silver nanoparticles using Hibiscus rosa sinensis

    Philip, Daizy

    2010-03-01

    Biological synthesis of gold and silver nanoparticles of various shapes using the leaf extract of Hibiscus rosa sinensis is reported. This is a simple, cost-effective, stable for long time and reproducible aqueous room temperature synthesis method to obtain a self-assembly of Au and Ag nanoparticles. The size and shape of Au nanoparticles are modulated by varying the ratio of metal salt and extract in the reaction medium. Variation of pH of the reaction medium gives silver nanoparticles of different shapes. The nanoparticles obtained are characterized by UV-vis, transmission electron microscopy (TEM), X-ray diffraction (XRD) and FTIR spectroscopy. Crystalline nature of the nanoparticles in the fcc structure are confirmed by the peaks in the XRD pattern corresponding to (1 1 1), (2 0 0), (2 2 0) and (3 1 1) planes, bright circular spots in the selected area electron diffraction (SAED) and clear lattice fringes in the high-resolution TEM image. From FTIR spectra it is found that the Au nanoparticles are bound to amine groups and the Ag nanoparticles to carboxylate ion groups.

  12. Boehmenan, a lignan from Hibiscus ficulneus, showed Wnt signal inhibitory activity.

    Shono, Takumi; Ishikawa, Naoki; Toume, Kazufumi; Arai, Midori A; Ahmed, Firoj; Sadhu, Samir K; Ishibashi, Masami

    2015-07-15

    The Wnt signal pathway modulates numerous biological processes, and its aberrant activation is related to various diseases. Therefore, inhibition of the Wnt signal may provide an effective (or efficient) strategy for these diseases. Cell-based luciferase assay targeting the Wnt signal (TOP assay) revealed that Hibiscus ficulneus extract inhibited the Wnt signal. The activity-guided isolation of the MeOH extract of H. ficulneus stems yielded four known (1-4) lignans along with myriceric acid (5). Compounds 1-4 potently inhibited the Wnt signal with TOPflash IC50 values of 1.0, 4.5, 6.3, and 1.9 μM, respectively. Compound 1 exhibited cytotoxicity against both Wnt-dependent (HCT116) and Wnt-independent (RKO) cells. Western blot analysis showed that 1 decreased the expression of full, cytosolic and nuclear β-catenin along with c-myc in STF/293 cells. Our results suggested that 1 may have inhibited the Wnt signal by decreasing β-catenin levels. Copyright © 2015 Elsevier Ltd. All rights reserved.

  13. Encapsulating anthocyanins from Hibiscus sabdariffa L. calyces by ionic gelation: Pigment stability during storage of microparticles.

    de Moura, Sílvia C S R; Berling, Carolina L; Germer, Sílvia P M; Alvim, Izabela D; Hubinger, Míriam D

    2018-02-15

    Hibiscus extract (HE) has a strong antioxidant activity and high anthocyanin content; it can be used as a natural pigment, also adding potential health benefits. The objective of this work was the microencapsulation of HE anthocyanin by ionic gelation (IG) using two techniques: dripping-extrusion and atomization, both by means of a double emulsion (HE/rapseed oil/pectin) and a cross-linked solution (CaCl 2 ). Particles (77-83% moisture content) were conditioned in acidified solution at 5, 15 and 25°C, absence of light, and evaluated for anthocyanins and color for 50-days. The median diameter (D 50 ) of the particles ranged from 78 to 1100μm and encapsulation efficiency ranged from 67.9 to 93.9%. The encapsulation caused higher temperature stability compared with the free extract. The half-life (t 1/2 ) values of the particles ranged from 7 (25°C) to 180days (5°C) for anthocyanins and from 25 (25°C) to 462days (5°C) for Chroma value. The IG increased the stability of HE anthocyanin. Both the dripping-extrusion and the atomization have shown to be feasible techniques. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. Purification, characterization and immunoregulatory activity of a polysaccharide isolated from Hibiscus sabdariffa L.

    Zheng, Daheng; Zou, Ye; Cobbina, Samuel Jerry; Wang, Wei; Li, Qian; Chen, Yao; Feng, Weiwei; Zou, Yanmin; Zhao, Ting; Zhang, Min; Yang, Liuqing; Wu, Xiangyang

    2017-03-01

    Hibiscus sabdariffa L. is not only used traditionally as a component of herbal drinks, beverages and flavoring agents but also as a herbal medicine in the drug industry. Bioactive polysaccharides are important constituents of H. sabdariffa that may contribute to the plant's beneficial effects. This study was designed to investigate the structural characteristics of a water-soluble polysaccharide from H. sabdariffa, HSP41, and its immunoregulatory activity on RAW264.7 cells. HSP41 was mainly composed of arabinose, xylose and mannose at a molar ratio of 1:1.34:15.6, with an average molecular weight of 3.3 × 10 5  Da. Fourier transform infrared (FTIR) spectra exhibited absorption peaks characteristic of HSP41. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) revealed the amorphous form and aggregation conformation of HSP41 respectively. HSP41 significantly induced interleukin 1β (IL-1β) and inducible nitric oxide synthase (iNOS) expression in RAW264.7 cells in vitro, promoting an increase in nuclear factor kB p65 (NF-kB p65) levels in the nucleus. The results indicated that HSP41 up-regulated the immune response by stimulating RAW264.7 cell activity. HSP41, a promising immunoregulator, possibly contributes to the health benefits of H. sabdariffa and might have potential applications in health food or medicine. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  15. Toxicity studies of the water extract from the calyces of Hibiscus sabdariffa L. in rats.

    Sireeratawong, Seewaboon; Itharat, Arunporn; Khonsung, Parirat; Lertprasertsuke, Nirush; Jaijoy, Kanjana

    2013-01-01

    Acute and chronic toxicities of the water extract from calyces of Hibiscus sabdariffa were studied in male and female rats. After 14 days of a single oral administration of test substance 5,000 mg/kg body weight, measurement of the body and organ weights, necropsy and health monitoring were performed. No signs and differences of the weights or behaviour compared to the control rats were observed. The results indicated that the single oral administration of H. sabdariffa extract in the amount of 5,000 mg/kg body weight does not produce acute toxicity. The chronic toxicity was determined by oral feeding both male and female rats daily with the extract at the doses of 50, 100, and 200 mg/kg body weight for 270 days. The examinations of signs, animal behaviour and health monitoring showed no defects in the test groups compared to the control groups. Both test and control groups (day 270th) and satellite group (day 298th) were analysed by measuring their final body and organ weights, taking necropsy, and examining haematology, blood clinical chemistry, and microanatomy. Results showed no differences from the control groups. Overall, our study demonstrated that an oral administration of H. sabdariffa extract at the doses of 50, 100 and 200 mg/kg body weight for 270 days does not cause chronic toxicity in rat.

  16. Antimutagenic Effect of Hibiscus sabdariffa L. Aqueous Extract on Rats Treated with Monosodium Glutamate

    Kerkhoff, Jacqueline; Vieira Júnior, Gerardo Magela; de Campos, Kleber Eduardo; Sugui, Marina Mariko

    2017-01-01

    Hibiscus sabdariffa L. is a plant of the Malvaceae family, commonly known as roselle. H. sabdariffa is known to contain antioxidant, cholesterol-lowering, antiobesity, insulin resistance reduction, antihypertensive, and skin cancer chemopreventive properties. This study evaluated the effects of H. sabdariffa aqueous extract against cyclophosphamide (CPA, 25 mg/Kg) induced damage to DNA in male Wistar rats by micronucleus test. Samples of H. sabdariffa calyx were obtained in the municipality of Barra do Garças, Mato Grosso, Brazil. The aqueous extract was prepared by infusion and each animal received a daily dose of 400 mg/Kg by gavage for 15 consecutive days of treatment. The presence of anthocyanins was confirmed by ferric chloride test and phenolic compounds using high-performance liquid chromatography, with emphasis on the identification of rutin. The animals were sacrificed by deepening of anaesthesia to obtain bone marrow and determination of the frequency of micronucleated polychromatic erythrocytes. The group treated with the aqueous extract of H. sabdariffa revealed a 91% reduction in micronucleus frequency when compared with the positive control group. Under the conditions tested, H. sabdariffa L. presented a protective effect to CPA-induced damage to DNA of the treated animals, and it is a potential candidate as a chemopreventive agent against carcinogenesis. PMID:28197528

  17. Hibiscus sabdariffa L. as a source of nutrients, bioactive compounds and colouring agents.

    Jabeur, Inès; Pereira, Eliana; Barros, Lillian; Calhelha, Ricardo C; Soković, Marina; Oliveira, M Beatriz P P; Ferreira, Isabel C F R

    2017-10-01

    The nutritional and bioactive composition of plants have aroused much interest not only among scientists, but also in people's daily lives. Apart from the health benefits, plants are a source of pigments that can be used as natural food colorants. In this work, the nutritional composition of Hibiscus sabdariffa L. was analysed, as well as its bioactive compounds and natural pigments. Glucose (sugar), malic acid (organic acid), α-tocopherol (tocopherol) and linoleic acid (fatty acid) were the major constituents in the corresponding classes. 5-(Hydroxymethyl) furfural was the most abundant non-anthocyanin compound, while delphinidin-3-O-sambubioside was the major anthocyanin both in its hydroethanolic extract and infusion. H. sabdariffa extracts showed antioxidant and antimicrobial activities, highlighting that the hydroethanol extract presents not only lipid peroxidation inhibition capacity, but also bactericidal/fungicidal inhibition ability for all the bacteria and fungi tested. Furthermore, both extracts revealed the absence of toxicity using porcine primary liver cells. The studied plant species was thus not only interesting for nutritional purposes but also for bioactive and colouring applications in food, cosmetic and pharmaceutical industries. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. CHARACTERISTICS OF PROBIOTIC KOUMISS FROM GOAT MILK WITH ADDITION OF ROSELLE EXTRACT (Hibiscus Sabdariffa Linn

    E. Nuraeni

    2015-04-01

    Full Text Available Goat milk and roselle (Hibiscus sabdariffa Linn are food ingredients with health functionalproperties. This research used Lactobacillus acidophilus RRAM-01 and Lactococcus lactis RRAM-01which had been proven as probiotic, and Saccharomyces cereviceae. Saccharomyces cereviseae wasused to produce specific flavor components in koumiss (bacterial fermentation product. The objectiveof this research was to study the characteristics of goat milk probiotic koumiss added that with roselleextract. The results showed that the addition of different concentration of roselle extract (0%, 0.5%, and1% in the goat milk probiotic koumiss had no effect on chemical and microbiological characteristics ofkoumiss. Goat milk processed into koumiss had ability to suppress the growth of coliform and hadstrong inhibition activity against Escherichia coli ATCC 25922 as tested by agar diffusion test.Microbiological analysis used in this research was the pour plate method. Population of lactic acidbacteria indicated that the product fulfilled standards as probiotics koumiss (1011 cfu/mL, coliform wasnot detected in the products, therefore the products met the criteria of The National Agency of Drug andFood Control (NA-DFC as probiotic functional food. Sensory evaluation was conducted by usinghedonic quality test. Based on the hedonic quality test, addition of different concentrations of roselleextract increased the intensity of red color and koumiss viscosity, but had no effect to the aroma andsourness of koumiss.

  19. Antimutagenic Effect of Hibiscus sabdariffa L. Aqueous Extract on Rats Treated with Monosodium Glutamate

    Ana Carla Guidini Valentini Gheller

    2017-01-01

    Full Text Available Hibiscus sabdariffa L. is a plant of the Malvaceae family, commonly known as roselle. H. sabdariffa is known to contain antioxidant, cholesterol-lowering, antiobesity, insulin resistance reduction, antihypertensive, and skin cancer chemopreventive properties. This study evaluated the effects of H. sabdariffa aqueous extract against cyclophosphamide (CPA, 25 mg/Kg induced damage to DNA in male Wistar rats by micronucleus test. Samples of H. sabdariffa calyx were obtained in the municipality of Barra do Garças, Mato Grosso, Brazil. The aqueous extract was prepared by infusion and each animal received a daily dose of 400 mg/Kg by gavage for 15 consecutive days of treatment. The presence of anthocyanins was confirmed by ferric chloride test and phenolic compounds using high-performance liquid chromatography, with emphasis on the identification of rutin. The animals were sacrificed by deepening of anaesthesia to obtain bone marrow and determination of the frequency of micronucleated polychromatic erythrocytes. The group treated with the aqueous extract of H. sabdariffa revealed a 91% reduction in micronucleus frequency when compared with the positive control group. Under the conditions tested, H. sabdariffa L. presented a protective effect to CPA-induced damage to DNA of the treated animals, and it is a potential candidate as a chemopreventive agent against carcinogenesis.

  20. Determination of antioxidant activity of Hibiscus sabdariffa and Croton caudatus in Saccharomyces cerevisiae model system.

    Subhaswaraj, Pattnaik; Sowmya, M; Bhavana, V; Dyavaiah, Madhu; Siddhardha, Busi

    2017-08-01

    From ancient times, plants and plant derived products are exploited as a prominent source of folkloric medicines with tremendous therapeutic potential for an array of health disorders. In the present study, ethanolic leaf extract of Hibiscus sabdariffa and Croton caudatus were evaluated for free radical scavenging activity in Saccharomyces cerevisiae model system. H. sabdariffa and C. caudatus showed tremendous DPPH free radical scavenging potential with an IC 50 value of 184.88 and 305.39 µg/mL respectively at a concentration of 500 µg/mL. The ethanolic leaf extract of H. sabdariffa and C. caudatus also showed significant hydoxyl radical scavenging and total antioxidant activity. Ascorbic acid was used as positive control. The in vitro antioxidant activity was further supported by in vivo studies using radical scavenging mechanism in S. cerevisiae wild type and its isogenic deletion strains sod1∆ and tsa1∆ . The mutant yeast cells substantially scavenged the stress generated by H 2 O 2 when supplemented with ethanolic leaf extract of H. sabdariffa and C. caudatus as evident from spot assays followed by fluorescence assay (DCF-DA) using fluorescence microscopic and intensity studies. H. sabdariffa and C.caudatus significantly neutralize the ROS level in yeast mutants with concomitant decrease in fluorescence intensity as compared to the untreated yeast cells. The results suggested the efficacy of H. sabdariffa and C. caudatus as potent antioxidants in yeast system and thus their futuristic applications in therapeutics.

  1. Occurrence of Root Rot and Vascular Wilt Diseases in Roselle (Hibiscus sabdariffa L.) in Upper Egypt

    Hassan, Naglaa; Shimizu, Masafumi

    2014-01-01

    Roselle (Hibiscus sabdariffa L.) family Malvaceae is an important crop used in food, cosmetics and pharmaceutics industries. Roselle is cultivated mainly in Upper Egypt (Qena and Aswan governorates) producing 94% of total production. Root rot disease of roselle is one of the most important diseases that attack both seedlings and adult plants causing serious losses in crop productivity and quality. The main objective of the present study is to identify and characterize pathogens associated with root rot and wilt symptoms of roselle in Qena, Upper Egypt and evaluate their pathogenicity under greenhouse and field condition. Fusarium oxysporum, Macrophomina phaseolina, Fusarium solani, Fusarium equiseti and Fusarium semitectum were isolated from the natural root rot diseases in roselle. All isolated fungi were morphologically characterized and varied in their pathogenic potentialities. They could attack roselle plants causing damping-off and root rot/wilt diseases in different pathogenicity tests. The highest pathogenicity was caused by F. oxysporum and M. phaseolina followed by F. solani. The least pathogenic fungi were F. equiseti followed by F. semitectum. It obviously noted that Baladi roselle cultivar was more susceptible to infection with all tested fungi than Sobhia 17 under greenhouse and field conditions. This is the first report of fungal pathogens causing root rot and vascular wilt in roselle in Upper Egypt. PMID:24808737

  2. Effect of the temperature on the spray drying of Roselle extracts (Hibiscus sabdariffa L.).

    Gonzalez-Palomares, Salvador; Estarrón-Espinosa, Mirna; Gómez-Leyva, Juan Florencio; Andrade-González, Isaac

    2009-03-01

    The effect of the drying temperature on the volatile components and sensory acceptance of the Roselle (Hibiscus sabdariffa) extract in powder was investigated. The Roselle extraction was carried out by maceration with 7 L of 30% ethanol (v/v), 560 g of fresh Roselle calyces for 168 h. The Roselle extracts were spray dried at different temperatures 150, 160, 170, 180, 190, 200 and 210 degrees C, giving different outlet values about yield and final moisture. The volatile compounds in Roselle extract and dried samples were performed using needles of solid phase microextraction (SPME) and gas chromatography/mass spectrometry (GC/MS HP-5890). Twenty volatile compounds were identified in the extracts among them terpenoids, esters, hydrocarbons and aldehydes. Fourteen volatile compounds were identified in the powder sample, but only ten were present in the Roselle extract. This indicates that some compounds were lost and some others were generated due to a degradation process. An acceptability sensory analysis showed that the best powder sample was the Roselle extract dehydrated using temperature between 190 degrees C and 200 degrees C (pRoselle extracts ranging from 3.4 to 3.9. It was concluded that the spray drying temperature of the Roselle extracts has an effect on the volatile compounds losses.

  3. Uricosuric effect of Roselle (Hibiscus sabdariffa) in normal and renal-stone former subjects.

    Prasongwatana, Vitoon; Woottisin, Surachet; Sriboonlue, Pote; Kukongviriyapan, Veerapol

    2008-05-22

    The Roselle (Hibiscus sabdariffa) was investigated for its uricosuric effect. A human model with nine subjects with no history of renal stones (non-renal stone, NS) and nine with a history of renal stones (RS) was used in this study. A cup of tea made from 1.5 g of dry Roselle calyces was provided to subjects twice daily (morning and evening) for 15 days. A clotted blood and two consecutive 24-h urine samples were collected from each subject three times: (1) at baseline (control); (2) on days 14 and 15 during the tea drinking period; and (3) 15 days after the tea drinking was stopped (washout). Serum and 24-h urinary samples were analyzed for uric acid and other chemical compositions related to urinary stone risk factors. All analyzed serum parameters were within normal ranges and similar; between the two groups of subjects and among the three periods. Vis-à-vis the urinary parameters, most of the baseline values for both groups were similar. After taking the tea, the trend was an increase in oxalate and citrate in both groups and uric acid excretion and clearance in the NS group. In the RS group, both uric acid excretion and clearance were significantly increased (pRoselle calyces. Since the various chemical constituents in Roselle calyces have been identified, the one(s) exerting this uricosuric effect need to be identified.

  4. Functional properties of roselle (Hibiscus sabdariffa L.) seed and its application as bakery product.

    Nyam, Kar-Lin; Leao, Sod-Ying; Tan, Chin-Ping; Long, Kamariah

    2014-12-01

    Roselle (Hibiscus sabdariffa L.) seed is a valuable food resource as it has an excellent source of dietary fibre. Therefore, this study examined the functional properties of roselle seeds. Replacement of cookie flour with roselle seed powder at levels of 0-30 % was investigated for its effect on functional and nutritional properties of cookies. Among the four formulations cookies, the most preferred by panelists was 20 % roselle seed powder cookie (F3), followed by 10 % roselle seed powder cookie (F2) and 30 % roselle seed powder cookie (F4). The least preferred formulation among all was control cookie (F1). Cookie with 20 % roselle seed powder added showed higher content of total dietary fibre (5.6 g/100 g) as compared with control cookie (0.90 g/100 g). Besides that, cookies incorporated with roselle seed powder exhibited improved antioxidant properties. Thus, roselle seed powder can be used as a dietary fibre source and developed as a functional ingredient in food products.

  5. Phenolic compounds of Hibiscus sabdariffa and influence of organic residues on its antioxidant and antitumoral properties.

    Formagio, A S N; Ramos, D D; Vieira, M C; Ramalho, S R; Silva, M M; Zárate, N A H; Foglio, M A; Carvalho, J E

    2015-01-01

    The aim of this study was to evaluate the phenolic and flavonoids contents and the antioxidant and antitumoral activity of leaf and calyx methanolic extracts from Hibiscus sabdariffa (roselle) cultivated with poultry litter and organosuper® under three modes of application. The total phenolic content in the each extract was determined using the Folin-Ciocalteu reagent and for aluminium chloride flavonoids. The antioxidant parameters were analyzed using a 2, 2-diphenyl-1-picrylhydrazyl (DPPH.) free radical scavenging assay. An antitumor colorimetric assay using sulforhodamine B. The highest contents of phenolic and flavonoids were observed in leaf extracts (389.98 and 104.52 mg g-1, respectively) and calyx extracts (474.09 and 148.35 mg g-1, respectively) from plants cultivated with organosuper®, although these values did not differ significantly from those observed for the other treatments. The average IC50 of leaves (43.48 μg mL-1) and calyces (37.15 μg mL-1) demonstrated that both have substances that may contribute to free radical scavenging action. The methanol extract from calyces showed significant selective activity against a leukemia line (K-562), with IC50 values of 0.12 mg mL-1 (organosuper®) and 1.16 mg mL-1 (poultry litter), with concentration-dependent, cytotoxic and cytocidal effects.

  6. Occurrence of Root Rot and Vascular Wilt Diseases in Roselle (Hibiscus sabdariffa L.) in Upper Egypt.

    Hassan, Naglaa; Shimizu, Masafumi; Hyakumachi, Mitsuro

    2014-03-01

    Roselle (Hibiscus sabdariffa L.) family Malvaceae is an important crop used in food, cosmetics and pharmaceutics industries. Roselle is cultivated mainly in Upper Egypt (Qena and Aswan governorates) producing 94% of total production. Root rot disease of roselle is one of the most important diseases that attack both seedlings and adult plants causing serious losses in crop productivity and quality. The main objective of the present study is to identify and characterize pathogens associated with root rot and wilt symptoms of roselle in Qena, Upper Egypt and evaluate their pathogenicity under greenhouse and field condition. Fusarium oxysporum, Macrophomina phaseolina, Fusarium solani, Fusarium equiseti and Fusarium semitectum were isolated from the natural root rot diseases in roselle. All isolated fungi were morphologically characterized and varied in their pathogenic potentialities. They could attack roselle plants causing damping-off and root rot/wilt diseases in different pathogenicity tests. The highest pathogenicity was caused by F. oxysporum and M. phaseolina followed by F. solani. The least pathogenic fungi were F. equiseti followed by F. semitectum. It obviously noted that Baladi roselle cultivar was more susceptible to infection with all tested fungi than Sobhia 17 under greenhouse and field conditions. This is the first report of fungal pathogens causing root rot and vascular wilt in roselle in Upper Egypt.

  7. Morphological characteristics and pathogenicity of fungi associated with Roselle (Hibiscus Sabdariffa) diseases in Penang, Malaysia.

    Eslaminejad, Touba; Zakaria, Maziah

    2011-11-01

    Roselle, or Jamaica sorrel (Hibiscus sabdariffa) is a popular vegetable in many tropical regions, cultivated for its leaves, seeds, stems and calyces which, the dried calyces are used to prepare tea, syrup, jams and jellies and as beverages. The main objectives of this study were to identify and characterise fungal pathogens associated with Roselle diseases based on their morphological and cultural characteristics and to determine the pathogenicity of four fungi infecting Roselle seedlings, namely Phoma exigua, Fusarium nygamai, Fusarium tgcq and Rhizoctonia solani in Penang. A total of 200 fungal isolates were obtained from 90 samples of symptomatic Roselle tissues. The isolates were identified based on cultural and morphological characteristics, as well as their pathogenicity. The fungal pathogen most frequently isolated was P. exigua (present in 45% of the samples), followed by F. nygamai (25%), Rhizoctonia solani (19%) and F. camptoceras (11%). Pathogenicity tests showed that P. exigua, F. nygamai, F. camptoceras and R. solani were able to infect both wounded and unwounded seedlings with different degrees of severity as indicated by the Disease severity (DS). R. solani was the most pathogenic fungus affecting both wounded and unwounded Roselle seedlings, followed by P. exigua that was highly pathogenic on wounded seedlings. F. nygamai was less pathogenic while the least pathogenic fungus was F. camptoceras, infecting only the unwounded seedlings but, surprisingly, not the wounded plants. Copyright © 2011 Elsevier Ltd. All rights reserved.

  8. Effects of Photoperiodism to The Growth Rate of Hibiscus sabdariffa L

    Wirdhatul Muslihatin

    2014-04-01

    Full Text Available Red roselle (Hibiscus saddariffa L. is a short day plant, this plant is only cultivated in a certain time period. There is a need to manipulate the environment where this plant can grow. The objective of this research was to study the influence of day length tothe relative growth rate (RGR of H.sabdariffaL. Day length treatment was carried out by covering plants by black cover. Day length treatments were given in 8, 9,10, 11 and 12 hours of light exposure. Parameters measured were RGR of height plant, diameter of stem, number, and color of leaves. Harvesting was carried out when the flower crown fell or petals furl (the age of plants reached 3-4 months. The result of this research showed that highest plant was obtained at 10 hours day length (128.20 cm, the largest stem diameter growth rate was obtained at 8 hours (1.79 cm, number of leaves at 10 hours (183.40,and green color of leaves on 12 hours (scale 4. The highest RGR was obtained at 8 hours for all parameters except the height of plant and the color of leaves. Plant height, diameter and number of leaves at 8,9,10, and 11 hours of day length did not show significance difference. This showed that roselle is a short day length plant that grow optimal under 12 hours of day length.

  9. Anthocyanins degradation during storage of Hibiscus sabdariffa extract and evolution of its degradation products.

    Sinela, André; Rawat, Nadirah; Mertz, Christian; Achir, Nawel; Fulcrand, Hélène; Dornier, Manuel

    2017-01-01

    Degradation parameters of two main anthocyanins from roselle extract (Hibiscus sabdariffa L.) stored at different temperatures (4-37°C) over 60days were determined. Anthocyanins and some of their degradation products were monitored and quantified using HPLC-MS and DAD. Degradation of anthocyanins followed first-order kinetics and reaction rate constants (k values), which were obtained by non-linear regression, showed that the degradation rate of delphinidin 3-O-sambubioside was higher than that of cyanidin 3-O-sambubioside with k values of 9.2·10(-7)s(-1) and 8.4·10(-7)s(-1) at 37°C respectively. The temperature dependence of the rate of anthocyanin degradation was modeled by the Arrhenius equation. Degradation of delphinidin 3-O-sambubioside (Ea=90kJmol(-1)) tended to be significantly more sensitive to an increase in temperature than cyanidin 3-O-sambubioside (Ea=80kJmol(-1)). Degradation of these anthocyanins formed scission products (gallic and protocatechuic acids respectively) and was accompanied by an increase in polymeric color index. Copyright © 2016 Elsevier Ltd. All rights reserved.

  10. Protective effect of Hibiscus sabdariffa against serum/glucose deprivation-induced PC12 cells injury

    Bakhtiari, Elham; Hosseini, Azar; Mousavi, Seyed Hadi

    2015-01-01

    Objectives: Findings natural products with antioxidant and antiapoptotic properties has been one of the interesting challenges in the search for the treatment of neurodegenerative diseases including ischemic stroke. Serum/glucose deprivation (SGD) has been used as a model for the understanding of the molecular mechanisms of neuronal damage during ischemia in vitro and for the expansion of neuroprotective drugs against ischemia-induced brain injury. Recent studies showed that Hibiscus sabdariffa exert pharmacological actions such as potent antioxidant. Therefore, in this study we investigated the protective effect of extract of H. sabdariffa against SGD-induced PC12 cells injury. Materials and Methods: Cells were pretreated with different concentrations of H. sabdariffa extract (HSE) for 2 hr, and then exposed to SGD condition for 6, 12 and 18 hr. Results: SGD caused a major reduction in cell viability after 6, 12, and 18 hr as compared with control cells (psabdariffa has the potential to be used as a new therapeutic approach for neurodegenerative disorders. PMID:26101756

  11. Hibiscus sabdariffa extract inhibits obesity and fat accumulation, and improves liver steatosis in humans.

    Chang, Hong-Chou; Peng, Chiung-Huei; Yeh, Da-Ming; Kao, Erl-Shyh; Wang, Chau-Jong

    2014-04-01

    Obesity is associated with a great diversity of diseases including non-alcoholic fatty liver disease. Our previous report suggested that Hibiscus sabdariffa extracts (HSE) had a metabolic-regulating and liver-protecting potential. In this study, we performed a clinical trial to further confirm the effect of HSE. Subjects with a BMI ≧ 27 and aged 18-65, were randomly divided into control (n = 17) and HSE-treated (n = 19) groups, respectively, for 12 weeks. Our data showed that consumption of HSE reduced body weight, BMI, body fat and the waist-to-hip ratio. Serum free fatty acid (FFA) was lowered by HSE. Anatomic changes revealed that HSE improved the illness of liver steatosis. Ingestion of HSE was well tolerated and there was no adverse effect during the trial. No alteration was found for serum α-amylase and lipase. The clinical effect should mainly be attributed to the polyphenols of HSE, since composition analysis showed that branched chain-amino acids, which is associated with obesity, is not obviously high. In conclusion, consumption of HSE reduced obesity, abdominal fat, serum FFA and improved liver steatosis. HSE could act as an adjuvant for preventing obesity and non-alcoholic fatty liver.

  12. Antipyretic activity of the extracts of Hibiscus sabdariffa calyces L. in experimental animals

    Wantana Reanmongkol

    2007-03-01

    Full Text Available The effects of the extracts from Hibiscus sabdariffa calyces L. (H. sabdariffa on nociceptive response using writhing, hot plate and formalin test in mice and the antipyretic activity in yeast-induced fever in rats, were examined. Anti-inflammatory activity was also investigated on carrageenin-induced paw edema in rats. No acute toxicity was observed in mice after oral administration of the ethanol and aqueous extract of H. sabdariffa calyces at the dose of 15 g/kg. Oral administration of the ethanol extract at the dose of 800 mg/kg significantly decreased the number of contortions and stretchings induced by acetic acid in mice. The aqueous extracts had no effect on this test. Neither the ethanol nor aqueous extract had an effect in the formalin and hot plate tests in mice. The ethanol and the vacuum dried extract of H. sabdariffa calyces (200-800 mg/kg, p.o. decreased the yeast-induced fever in rats. The H. sabdariffa extract had no effect on carrageenininduced paw edema in rats. These results suggest that the ethanol and aqueous extract (vacuum dry of H. sabdariffa calyces possess antipyretic action through mechanisms that are different from that of aspirin.

  13. Microbial Quality and Proximate Composition of Dried Hibiscus sabdariffa Calyxes in Uyo, Eastern Nigeria

    Adebayo-tayo, B. C.

    2009-01-01

    Full Text Available Dried Hibiscus sabdarifa calyxes collected from different markets in Uyo, Eastern Nigeria were evaluated for microbial quality, Aflatoxin contamination and proximate composition. The results showed that all the calyxes were contaminated with microorganism. The total bacteria count ranged from 5.0 x 10^3 to 8.1 x 10^4 cfu/g in which the highest count was obtained from dried calyxes from Itam Market. Coliform were not detected in most of the sample except samples from Uyo main market and Ikot Ekpene market in which the coliform level is below the acceptable limit. Salmonella/Shigella was not detected in the sample. The fungi count ranged from 3.4 x 10^4 to 7.3 x 10^4. The associated bacteria were Staphylococcus aureus, Bacillus subtilis, Bacillus sp. Enterococuss faecalis, Micrococcus sp. and Klebsiella sp. The associated fungi were Aspergillus flavus, A. terreus, A. glaucus, Penicillium citrinum, Fusarium oxysporum, Rhizopus sp. and Mucor sp. A. glaucus had the highest frequency of occurrence among the isolated fungi. Out of the sample obtained Aflatoxin B1 was detected in two samples and it ranged from 1.57 to 17.8 /µg/kg. The proximate analysis revealed that the crude protein ranged from 8.34 – 9.97%, crude fibre (7.26 – 7.82% and fat (8.51 – 9.26%. The moisture content ranged from 13.13 – 14.85%.

  14. Identification and Characterization of Roseltide, a Knottin-type Neutrophil Elastase Inhibitor Derived from Hibiscus sabdariffa

    Loo, Shining; Kam, Antony; Xiao, Tianshu; Nguyen, Giang K. T.; Liu, Chuan Fa; Tam, James P.

    2016-01-01

    Plant knottins are of therapeutic interest due to their high metabolic stability and inhibitory activity against proteinases involved in human diseases. The only knottin-type proteinase inhibitor against porcine pancreatic elastase was first identified from the squash family in 1989. Here, we report the identification and characterization of a knottin-type human neutrophil elastase inhibitor from Hibiscus sabdariffa of the Malvaceae family. Combining proteomic and transcriptomic methods, we identified a panel of novel cysteine-rich peptides, roseltides (rT1-rT8), which range from 27 to 39 residues with six conserved cysteine residues. The 27-residue roseltide rT1 contains a cysteine spacing and amino acid sequence that is different from the squash knottin-type elastase inhibitor. NMR analysis demonstrated that roseltide rT1 adopts a cystine-knot fold. Transcriptome analyses suggested that roseltides are bioprocessed by asparagine endopeptidases from a three-domain precursor. The cystine-knot structure of roseltide rT1 confers its high resistance against degradation by endopeptidases, 0.2 N HCl, and human serum. Roseltide rT1 was shown to inhibit human neutrophil elastase using enzymatic and pull-down assays. Additionally, roseltide rT1 ameliorates neutrophil elastase-stimulated cAMP accumulation in vitro. Taken together, our findings demonstrate that roseltide rT1 is a novel knottin-type neutrophil elastase inhibitor with therapeutic potential for neutrophil elastase associated diseases. PMID:27991569

  15. Inhibition of calcium oxalate crystal deposition on kidneys of urolithiatic rats by Hibiscus sabdariffa L. extract.

    Laikangbam, Reena; Damayanti Devi, M

    2012-06-01

    The present study aims at systematic evaluation of the calyces of Hibiscus sabdariffa to establish its scientific validity for anti-urolithiatic property using ethylene glycol-induced hyperoxaluria model in male albino rats. Administration of a mixture of 0.75% ethylene glycol and 2% ammonium chloride resulted in hyperoxaluria as well as increased renal excretion of calcium and phosphate. The decrease in the serum calcium concentration indicates an increased calcium oxalate formation. Supplementation of aqueous extract of H. sabdariffa at different doses (250, 500 and 750 mg/kg body weight) significantly lowered the deposition of stone-forming constituents in the kidneys and serum of urolithiatic rats. These findings have been confirmed through histological investigations. Results of in vivo genotoxicity testing showed no significant chromosomal aberrations in the bone marrow cells of ethylene glycol-induced rats. The plant extracts at the doses investigated induced neither toxic nor lethal effects and are safe. It can be concluded that the calyces of H. sabdariffa are endowed with anti-urolithiatic activity and do not have genotoxic effects. Thus, it can be introduced in clinical practices and medicine in the form of orally administered syrup after further investigations and clinical trials.

  16. Infusion of Hibiscus sabdariffa L. Modulates Oxidative Stress in Patients with Marfan Syndrome.

    Soto, María Elena; Zuñiga-Muñoz, Alejandra; Guarner Lans, Verónica; Duran-Hernández, Erendira Janet; Pérez-Torres, Israel

    2016-01-01

    Marfan syndrome (MFS) is associated with progressive aortic dilatation, endothelial dysfunction, and oxidative stress that contribute to the early acute dissection of the vessel and can end up in rupture of the aorta and sudden death. Many studies have described that the organic acids from Hibiscus sabdariffa Linne (HSL) calyces increase cellular antioxidant capacity and decrease oxidative stress. Here we evaluate if the antioxidant properties of HSL infusion improve oxidative stress in MFS patients. Activities of extra cellular super oxide dismutase (ECSOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), glutathione reductase (GSSG-R), glutathione (GSH), lipid peroxidation (LPO) index, total antioxidant capacity (TAC), and ascorbic acid were determined in plasma from MFS patients. Values before and after 3 months of the treatment with 2% HSL infusion were compared in control and MFS subjects. After treatment, there was a significant decrease in ECSOD (p = 0.03), EGPx (p = 0.04), GST (p = 0.03), GSH (p = 0.01), and TAC and ascorbic acid (p = 0.02) but GSSG-R activity (p = 0.04) and LPO (p = 0.02) were increased in MFS patients in comparison to patients receiving the HSL treatment and C subjects. Therefore, the infusion of HSL calyces has antioxidant properties that allow an increase in antioxidant capacity of both the enzymatic and nonenzymatic systems, in the plasma of the MSF patients.

  17. Safety assessment of Hibiscus sabdariffa after maternal exposure on male reproductive parameters in rats.

    de Arruda, Aline; Cardoso, Claudia Andrea L; Vieira, Maria do Carmo; Arena, Arielle Cristina

    2016-01-01

    Hibiscus sabdariffa L. (Malvaceae) is a species widely used in folk medicine for the treatment of some disorders. This study evaluated the effects of H. sabdariffa (HS) on the development of the male reproductive tract in rats following in utero exposure. Pregnant rats received 250 or 500 mg/kg of HS extract or vehicle from gestational day 12 until day 21 of lactation. Both doses of HS increased the body weight of male offspring at weaning, without compromising the puberty onset parameters. At puberty, there was a significant increase in the vas deferens absolute weight and a significant reduction in the relative weight of kidney at higher dose. These animals also presented a significant reduction in the sperm number in the caput/corpus of epididymis after exposure to both doses and a reduction in the sperm number in the cauda epididymis for the lower dose. At adulthood, the highest dose significantly reduced the sperm production in relation to controls and both doses provoked a reduction in the relative sperm number in the epididymis without affecting the sperm morphology. These findings demonstrated that maternal exposure to H. sabdariffa can adversely influence the male reproductive system in rats.

  18. Fatty acid composition, anti-inflammatory and analgesic activities of Hibiscus sabdariffa Linn. seeds

    Sumaia Awad Elkariem Ali

    2014-06-01

    Full Text Available Hibiscus (H. sabdariffa (commonly known as “Karkadeh” in Arabic is widely used in various pharmacological applications in Sudan. The present study was carried out to investigate the anti-inflammatory and analgesic activities of H. sabdariffa seed extracts using rat models. In acute anti-inflammatory models, oral administration of petroleum ether extract of H. sabdariffa seeds inhibited the hind paw edema (p<0.01 which was induced by carrageenan. The petroleum ether extract exhibited significant (p<0.01 inhibition of vascular permeability in rats induced by intraperitoneal injection of acetic acid (0.6%. In cotton pellet granuloma method, the petroleum ether extract of H. sabdariffa seed showed significant inhibition of granuloma. The extract reduced (p<0.001 abdominal constrictions which was induced by injection of acetic acid (0.7%. Analysis of seed oil of H. sabdariffa using Gas Chromatography revealed the presence of three fatty acids; these were linolelaidic acid, arachidic acid, and palmitic acid. In conclusion, H. sabdariffa seeds possess anti-inflammatory and analgesic activities in rat model.

  19. Antitumoral Effect of Hibiscus sabdariffa on Human Squamous Cell Carcinoma and Multiple Myeloma Cells.

    Malacrida, Alessio; Maggioni, Daniele; Cassetti, Arianna; Nicolini, Gabriella; Cavaletti, Guido; Miloso, Mariarosaria

    2016-10-01

    Cancer is a leading cause of death worldwide. Despite therapeutic improvements, some cancers are still untreatable. Recently there has been an increasing interest in the use of natural substances for cancer prevention and treatment. Hibiscus sabdariffa (HS) is a plant, belonging to Malvaceae family, widespread in South Asia and Central Africa. HS extract (HSE) used in folk medicine, gained researchers' interest thanks to its antioxidant, anti-inflammatory, and chemopreventive properties. In the present study, we initially assessed HSE effect on a panel of human tumor cell lines. Then we focused our study on the following that are most sensitive to HSE action cell lines: Multiple Myeloma (MM) cells (RPMI 8226) and Oral Squamous Cell Carcinoma (OSCC) cells (SCC-25). In both RPMI 8226 and SCC-25 cells, HSE impaired cell growth, exerted a reversible cytostatic effect, and reduced cell motility and invasiveness. We evaluated the involvement of MAPKs ERK1/2 and p38 in HSE effects by using specific inhibitors, U0126 and SB203580, respectively. For both SCC-25 and RPMI 8226, HSE cytostatic effect depends on p38 activation, whereas ERK1/2 modulation is crucial for cell motility and invasiveness. Our results suggest that HSE may be a potential therapeutic agent against MM and OSCC.

  20. Antibacterial efficiency of the Sudanese Roselle (Hibiscus sabdariffa L.), a famous beverage from Sudanese folk medicine

    Abdallah, Emad Mohamed

    2016-01-01

    Background: Hibiscus sabdariffa L. is a plant native to tropical Africa and intensively cultivated in Sudan. Its calyces are widely consumed with many uses in Sudanese folk medicine. Materials and Methods: The dried calyces of H. sabdariffa were subjected to soak in 80% v/v methanol to get the methanolic extract, which was tested against five Gram-negative and three Gram-positive referenced bacterial strains using disc diffusion method. Selected bioactive phytochemical compounds were also investigated using qualitative methods. Results: The results of the antibacterial test indicate that the methanol extract of H. sabdariffa calyces contained effective antibacterial agent(s), revealed a considerable zone of inhibition against all tested Gram-negative and Gram-positive bacteria, and it was a competitor to gentamicin and greatly higher than penicillin which showed weak or no effect. Conclusion: The results of current investigation support the folk medicine application of this plant against different microbial ailments and suggest it as a promising source for new antibacterial agents. PMID:27104041