WorldWideScience

Sample records for nuclear mrna surveillance

  1. Dissecting mechanisms of nuclear mRNA surveillance in THO/sub2 complex mutants

    DEFF Research Database (Denmark)

    Rougemaille, Mathieu; Gudipati, Rajani Kanth; Olesen, Jens Raabjerg

    2007-01-01

    by appending oligo(A)-tails onto structured substrates. Another role of the nuclear exosome is that of mRNA surveillance. In strains harboring a mutated THO/Sub2p system, involved in messenger ribonucleoprotein particle biogenesis and nuclear export, the exosome-associated 3' 5' exonuclease Rrp6p is required...

  2. Dissecting mechanisms of nuclear mRNA surveillance in THO/sub2 complex mutants

    DEFF Research Database (Denmark)

    Rougemaille, Mathieu; Gudipati, Rajani K; Olesen, Jens Raabjerg

    2007-01-01

    The nuclear exosome is involved in numerous RNA metabolic processes. Exosome degradation of rRNA, snoRNA, snRNA and tRNA in Saccharomyces cerevisiae is activated by TRAMP complexes, containing either the Trf4p or Trf5p poly(A) polymerase. These enzymes are presumed to facilitate exosome access...... is required for both retention and degradation of nuclear restricted mRNAs. We show here that Trf4p, in the context of TRAMP, is an mRNA surveillance factor. However, unlike Rrp6p, Trf4p only partakes in RNA degradation and not in transcript retention. Surprisingly, a polyadenylation-defective Trf4p protein...

  3. Surveillance of nuclear power reactors

    International Nuclear Information System (INIS)

    Marini, J.

    1983-01-01

    Surveillance of nuclear power reactors is now a necessity imposed by such regulatory documents as USNRC Regulatory Guide 1.133. In addition to regulatory requirements, however, nuclear reactor surveillance offers plant operators significant economic advantages insofar as a single day's outage is very costly. The economic worth of a reactor surveillance system can be stated in terms of the improved plant availability provided through its capability to detect incidents before they occur and cause serious damage. Furthermore, the TMI accident has demonstrated the need for monitoring certain components to provide operators with clear information on their functional status. In response to the above considerations, Framatome has developed a line of products which includes: pressure vessel leakage detection systems, loose part detection systems, component vibration monitoring systems, and, crack detection and monitoring systems. Some of the surveillance systems developed by Framatome are described in this paper

  4. Reviewing surveillance activities in nuclear power plants

    International Nuclear Information System (INIS)

    1989-03-01

    This document provides guidance to Operational Safety Review Teams (OSARTs) for reviewing surveillance activities at a nuclear power plant. In addition, the document contains reference material to support the review of surveillance activities, to assist within the Technical Support area and to ensure consistency between individual reviews. Drafts of the document have already been used on several OSART missions and found to be useful. The document first considers the objectives of an excellent surveillance programme. Investigations to determine the quality of the surveillance programme are then discussed. The attributes of an excellent surveillance programme are listed. Advice follows on how to phrase questions so as to obtain an informative response on surveillance features. Finally, specific equipment is mentioned that should be considered when reviewing functional tests. Four annexes provide examples drawn from operating nuclear power plants. They were selected to supplement the main text of the document with the best international practices as found in OSART reviews. They should in no way limit the acceptance and development of alternative approaches that lead to equivalent or better results. Refs, figs and tabs

  5. Quality surveillance at nuclear power plants

    International Nuclear Information System (INIS)

    Deviney, D.E.

    1990-01-01

    Quality surveillance (QS) of nuclear power plants has been occurring for a number of years and is growing in importance as a management tool for assuring that power plants are operated and maintained safely. Quality surveillance can be identified by many terms, such as monitoring, assessment, technical audits, and others. The name given to the function is not important. Quality surveillance at nuclear power plants developed out of a need. Historically, audits were performed to verify compliance to quality program requirements. Verification of day-to-day implementation of activities was not being performed. This left a void in verification activities since inspections were mainly directed at hardware verification. Quality surveillance, therefore, was born out of a need to fill this void in verification. This paper discusses quality surveillance definition; objectives of QS, activities considered for QS, personnel performing QS. As in any human endeavor, people and the attitudes of those people make a program succeed or fail. In the case of QS this is even more critical because of the overview and exposure given to the nuclear industry. Properly trained and experienced personnel performing QS combined with the right attitude contribute to the successful performance of a QS. This is only one side of the success equation, however; acceptance of and actions taken by plant management establish the total success of a QS program

  6. Nuclear Imprisonment: Viral Strategies to Arrest Host mRNA Nuclear Export

    Science.gov (United States)

    Kuss, Sharon K.; Mata, Miguel A.; Zhang, Liang; Fontoura, Beatriz M. A.

    2013-01-01

    Viruses possess many strategies to impair host cellular responses to infection. Nuclear export of host messenger RNAs (mRNA) that encode antiviral factors is critical for antiviral protein production and control of viral infections. Several viruses have evolved sophisticated strategies to inhibit nuclear export of host mRNAs, including targeting mRNA export factors and nucleoporins to compromise their roles in nucleo-cytoplasmic trafficking of cellular mRNA. Here, we present a review of research focused on suppression of host mRNA nuclear export by viruses, including influenza A virus and vesicular stomatitis virus, and the impact of this viral suppression on host antiviral responses. PMID:23872491

  7. Surveillance system for nuclear power plants

    International Nuclear Information System (INIS)

    Mizeracki, M.T.

    1981-01-01

    This paper describes an integrated surveillance system for nuclear power plant application. The author explores an expanded role for closed circuit television, with remotely located cameras and infrared scanners as the basic elements. The video system, integrated with voice communication, can enhance the safe and efficient operation of the plant, by improving the operator's knowledge of plant conditions. 7 refs

  8. Atucha I nuclear power plant surveillance programme

    Energy Technology Data Exchange (ETDEWEB)

    Jinchuk, D [Comision Nacional de Energia Atomica, Buenos Aires (Argentina)

    1994-12-31

    After a review of the main characteristics of the Atucha I nuclear power plant and its pressure vessel, the embrittlement surveillance capsules and the irradiation conditions are described; Charpy impact tests and tensile tests were performed on the irradiated samples, and results are discussed and compared to theoretical calculations: transition temperature shifts, displacement per atom values. 6 refs., 16 figs., 7 tabs.

  9. Atucha I nuclear power plant surveillance programme

    International Nuclear Information System (INIS)

    Jinchuk, D.

    1993-01-01

    After a review of the main characteristics of the Atucha I nuclear power plant and its pressure vessel, the embrittlement surveillance capsules and the irradiation conditions are described; Charpy impact tests and tensile tests were performed on the irradiated samples, and results are discussed and compared to theoretical calculations: transition temperature shifts, displacement per atom values. 6 refs., 16 figs., 7 tabs

  10. Surveillance robot for nuclear power plants

    International Nuclear Information System (INIS)

    White, J.R.; Harvey, H.W.; Satterlee, P.E. Jr.

    1985-01-01

    A demonstration project to determine the feasibility and cost-effectiveness of mobile surveillance robots in nuclear power plants is being conducted by the Remote Technology Corporation (REMOTEC) for the US Nuclear Regulatory Commission. Phase I of the project was completed in March 1984 and included a survey of currently used robotic equipment and the development of a robotics application methodology. Three Tennessee Valley Authority plants were analyzed to identify specific plant areas with a high potential for surveillance robotics. Based on these results, a number of robotic system applications were prepared and evaluated for cost-effectiveness. The system with the highest potential, a mobile surveillance robot, was selected for fabrication and in-plant demonstration testing in a phase II effort. The design, fabrication, and assembly of SURBOT has been completed and cold testing is in process. It will be installed at the Browns Ferry Nuclear Plant early in 1986 for demonstration testing. Current projections are that SURBOT can be used in approx.40 rooms within the auxiliary building and will have annual savings of over 100 person-rem exposure, 1000 sets of C-zone clothing, and 1000 person-hours of labor

  11. Nuclear Imprisonment: Viral Strategies to Arrest Host mRNA Nuclear Export

    Directory of Open Access Journals (Sweden)

    Beatriz M. A. Fontoura

    2013-07-01

    Full Text Available Viruses possess many strategies to impair host cellular responses to infection. Nuclear export of host messenger RNAs (mRNA that encode antiviral factors is critical for antiviral protein production and control of viral infections. Several viruses have evolved sophisticated strategies to inhibit nuclear export of host mRNAs, including targeting mRNA export factors and nucleoporins to compromise their roles in nucleo-cytoplasmic trafficking of cellular mRNA. Here, we present a review of research focused on suppression of host mRNA nuclear export by viruses, including influenza A virus and vesicular stomatitis virus, and the impact of this viral suppression on host antiviral responses.

  12. Nuclear surveillance of mRNP formation

    DEFF Research Database (Denmark)

    Jensen, Torben Heick

    Proper formation of mRNP requires co-transcriptional loading of proteins onto nascent transcripts. Mutations in several genes involved in mRNA processing, mRNP assembly and nuclear export lead to production of aberrant mRNPs that are retained in transcription site-associated foci. Retention...... and degradation of transcripts depend on the nuclear exosome of 3’-5’ exonucleases.We have studied connections between mRNP assembly and quality control in the yeast S. cerevisiae using mutants of the THO complex. THO is implicated in co-transcriptional mRNP assembly, but its precise role is not known. Genetic...... and biochemical data now show that a defective THO complex negatively impacts mRNA 3’-end processing. We are currently trying to understand the relationship between this phenomenon and mRNP quality control. Retention of mRNP in THO mutants is dependent on the nuclear exosome component Rrp6p. Using the solved...

  13. Tritium surveillance around nuclear facilities in Japan

    International Nuclear Information System (INIS)

    Inoue, Y.; Kasida, Y.

    1978-01-01

    In order to measure the tritium levels in the environmental water around the nuclear facilities, the tritium surveillance program began in 1967 locally at Tsuruga and Mihama districts. Nowadays it has been expanded to the ten commercial nuclear power stations and three nuclear facilities. For samples whose tritium concentration is believed less than about 100 pCi/l, they were electrolytically enriched, and then counted by the liquid scintillation counter. Some of samples believed higher than 100 pCi/l were analysed without any enrichment by the low background liquid scintillation counters, Aloka LB 600 or Aloka LB 1. The results of each station are listed in Table. The sampling points corresponding to each results are shown in Figure. Tritium from the effluent was not reflected in all the land water and the tap water around the nuclear power stations and the nuclear facilities. Tritium concentration in rivers, streams, and reservoirs (pools) decreased exponentially from about 600 pCi/l in 1967 to about 150 pCi/l in 1972 at Tsuruga and Mihama, and 360 pCi/l in 1968 to 120 pCi/l in 1973 at Genkai, with the half life of about 2.5 years in both cases. After around 1972, tritium levels of river system in all districts of Japan kept nearly constant up to the end of 1975 and they were in the range from 100 to 300 pCi/l corresponding to the districts. Thereafter, it seems to start to decrease again in 1976. Sea water sampled at the intake of the station or on the seashore far from the outlet was regarded not to be influenced by the effluent from the nuclear reactors or facilities. Tritium concentration in these coastal waters decreased from 100 - 300 pCi/l in 1971 to 30 - 40 pCi/l in 1972 in Fukushima, Ibaraki and Fukui prefectures. (author)

  14. Nuclear safety and radiation protection surveillance in different countries. Switzerland

    International Nuclear Information System (INIS)

    1993-01-01

    The information and historical review on the Nuclear Surveillance in Switzerland has been presented. Special attention has been paid on: general tasks and responsibility of the Nuclear Surveillance, its organization structures, legal aspects, regulations and recommendations governing all nuclear activities in Switzerland, licensing processes and their procedures, inspections and control functions as well as international cooperation in the field of nuclear safety and environment protection

  15. Surveillance of irradiation embrittlement of nuclear reactor pressure vessels

    International Nuclear Information System (INIS)

    Najzer, M.

    1982-01-01

    Surveillance of irradiation embrittlement of nuclear reactor pressure vessels is briefly discussed. The experimental techniques and computer programs available for this work at the J. Stefan Institute are described. (author)

  16. Evaluation and surveillance of radioactive releases of nuclear installations

    International Nuclear Information System (INIS)

    Hartmann, Ph.

    2002-01-01

    The two days organised by the section Environment of the SFRP have to objective to connect experts in radiation protection in order to debate around the following questions: the actual evaluations modes of releases impacts from nuclear installations, the organisation of the surveillance, to favour the implication of local actors in the evaluation and surveillance around nuclear facilities, the evolutions to envisage. (N.C.)

  17. Assessing mRNA nuclear export in mammalian cells by microinjection.

    Science.gov (United States)

    Lee, Eliza S; Palazzo, Alexander F

    2017-08-15

    The nuclear export of mRNAs is an important yet little understood part of eukaryotic gene expression. One of the easiest methods for monitoring mRNA export in mammalian tissue culture cells is through the microinjection of DNA plasmids into the nucleus and monitoring the distribution of the transcribed product over time. Here we describe how to setup a microscope equipped with a micromanipulator used in cell microinjections, and we explain how to perform a nuclear mRNA export assay and obtain the nuclear export rate for any given mRNA. Copyright © 2017 Elsevier Inc. All rights reserved.

  18. On some aspects of nuclear safety surveillance and review

    International Nuclear Information System (INIS)

    Li Ganjie; Zhu Hong; Zhou Shanyuan

    2004-01-01

    Five aspects of the nuclear safety surveillance and review are discussed: Strict implementation of nuclear safety regulation, making the nuclear safety surveillance and review more normalization, procedurization, scientific decision-making; Strictly requiring the applicant to comply with the requirements of codes, do not allowing the utilization of mixing of codes; Properly controlling the strictness for the review on significant non-conformance; Strengthening the co-operation between regional offices and technical support units, Properly treat the relations between administrational management unit and technical support units. (authors)

  19. Three-Dimensional Mapping of mRNA Export through the Nuclear Pore Complex

    Directory of Open Access Journals (Sweden)

    Steven J. Schnell

    2014-11-01

    Full Text Available The locations of transcription and translation of mRNA in eukaryotic cells are spatially separated by the nuclear envelope (NE. Plenty of nuclear pore complexes (NPCs embedded in the NE function as the major gateway for the export of transcribed mRNAs from the nucleus to the cytoplasm. Whereas the NPC, perhaps one of the largest protein complexes, provides a relatively large channel for macromolecules to selectively pass through it in inherently three-dimensional (3D movements, this channel is nonetheless below the diffraction limit of conventional light microscopy. A full understanding of the mRNA export mechanism urgently requires real-time mapping of the 3D dynamics of mRNA in the NPC of live cells with innovative imaging techniques breaking the diffraction limit of conventional light microscopy. Recently, super-resolution fluorescence microscopy and single-particle tracking (SPT techniques have been applied to the study of nuclear export of mRNA in live cells. In this review, we emphasize the necessity of 3D mapping techniques in the study of mRNA export, briefly summarize the feasibility of current 3D imaging approaches, and highlight the new features of mRNA nuclear export elucidated with a newly developed 3D imaging approach combining SPT-based super-resolution imaging and 2D-to-3D deconvolution algorithms.

  20. An IKBS approach to surveillance for naval nuclear submarine propulsion

    International Nuclear Information System (INIS)

    Cadas, C.N.; Bowskill, J.; Mayfield, T.; Clarke, J.C.

    1993-01-01

    This paper describes work being carried out to develop an IKBS for use in surveillance of naval nuclear submarine propulsion plant. In recent years, modern process plants have increased automation and installed surveillance equipment while reducing the level of manpower operating and monitoring the plant. As a result, some of the local watchkeeping tasks have been transferred to control room operators, and the data reduction and warning filtering expertise inherent in local plant operators has been lost, while an additional workload has been placed upon operators. The surveillance systems installed to date have therefore been less usable than anticipated. The solution being achieved for submarine power plant is to introduce IKBS into surveillance to replace lost expertise, i.e. to return to a situation in which operators receive small amounts of high quality information rather than large amounts of low quality information

  1. An IKBS approach to surveillance for naval nuclear submarine propulsion

    International Nuclear Information System (INIS)

    Cadas, C.N.; Bowskill, J.; Mayfield, T.; Clarke, J.C.

    1995-01-01

    This Paper describes work being carried out to develop an intelligent knowledge-based system (IKBS) for use in the surveillance of naval nuclear submarine propulsion plant. In recent years, modern process plants have increased automation and installed surveillance equipment while reducing the level of manpower operating and monitoring the plant. As a result, some of the local watchkeeping tasks have been transferred to control room operators, and the data reduction and warning filtering expertise inherent in local plant operators has been lost, while an additional workload has been placed upon operators. The surveillance systems installed to date have therefore been less usable than anticipated. The solution being achieved for submarine power plant is to introduce IKBS into surveillance to replace lost expertise and return to a situation in which operators receive small amounts of high quality information rather than large amounts of low quality information. (author)

  2. Testing of mobile surveillance robot at a nuclear power plant

    International Nuclear Information System (INIS)

    White, J.R.; Harvey, H.W.; Farnstrom, K.A.

    1987-01-01

    In-plant testing of a mobile surveillance robot (SURBOT) was performed at the Browns Ferry Nuclear Plant by TVA personnel. The results verified that SURBOT can be used for remote surveillance in 54 separate controlled radiation rooms at the plant. High-quality color video, audio, and other data are collected, digitized by an on-board computer, and transmitted through a cable to the control console for real-time display and videotaping. TVA projects that the use of SURBOT for surveillance during plant operation will produce annual savings of about 100 person-rem radiation exposure and $200,000 in operating costs. Based on the successful results of this program, REMOTEC is now commercializing the SURBOT technology on both wheeled and tracked mobile robots for use in nuclear power plants and other hazardous environments

  3. The State Surveillance over Nuclear Safety of Nuclear Facilities Act No. 28/1984

    International Nuclear Information System (INIS)

    1995-01-01

    The Act lays down responsibilities of the Czechoslovak Atomic Energy Commission in the field of state surveillance over nuclear safety of nuclear facilities; determines the responsibilities of nuclear safety inspectors in their inspection activities; specifies duties of bodies and corporations responsible for nuclear safety of nuclear facilities; stipulates the obligation to set up emergency plans; and specifies penalties imposed on corporations and individuals for noncompliance with nuclear safety provisions. The Act entered into force on 4 April 1984. (J.B.)

  4. New developments in seismic surveillance of nuclear power plants

    Energy Technology Data Exchange (ETDEWEB)

    Anesa, Franco; Vavassori, Maurizio [Instrumentation and Systems Department, ISMES, Bergamo (Italy); Casirati, Mario [Dynamic Department, ISMES, Bergamo (Italy)

    1988-07-01

    In the present article, new trends and development of ISMES techniques and methods relating to the seismic surveillance of nuclear power plants, are described. The experience gained in this field is described, and particular attention is paid to the new trend in surveys and instrumentation design criteria (from both the hardware and software point of view). (author)

  5. New developments in seismic surveillance of nuclear power plants

    International Nuclear Information System (INIS)

    Anesa, Franco; Vavassori, Maurizio; Casirati, Mario

    1988-01-01

    In the present article, new trends and development of ISMES techniques and methods relating to the seismic surveillance of nuclear power plants, are described. The experience gained in this field is described, and particular attention is paid to the new trend in surveys and instrumentation design criteria (from both the hardware and software point of view). (author)

  6. Characterization of the ptr5+ gene involved in nuclear mRNA export in fission yeast

    International Nuclear Information System (INIS)

    Watanabe, Nobuyoshi; Ikeda, Terumasa; Mizuki, Fumitaka; Tani, Tokio

    2012-01-01

    Highlights: ► We cloned the ptr5 + gene involved in nuclear mRNA export in fission yeast. ► The ptr5 + gene was found to encode nucleoporin 85 (Nup85). ► Seh1p and Mlo3p are multi-copy suppressors for the ptr5 mutation. ► Ptr5p/Nup85p functions in nuclear mRNA export through the mRNA export factor Rae1p. ► Ptr5p/Nup85p interacts genetically with pre-mRNA splicing factors. -- Abstract: To analyze the mechanisms of mRNA export from the nucleus to the cytoplasm, we have isolated eleven mutants, ptr [poly(A) + RNA transport] 1 to 11, which accumulate poly(A) + RNA in the nucleus at a nonpermissive temperature in Schizosaccharomyces pombe. Of those, the ptr5–1 mutant shows dots- or a ring-like accumulation of poly(A) + RNA at the nuclear periphery after shifting to the nonpermissive temperature. We cloned the ptr5 + gene and found that it encodes a component of the nuclear pore complex (NPC), nucleoporin 85 (Nup85). The ptr5–1 mutant shows no defects in protein transport, suggesting the specific involvement of Ptr5p/Nup85p in nuclear mRNA export in S. pombe. We identified Seh1p, a nucleoporin interacting with Nup85p, an mRNA-binding protein Mlo3p, and Sac3p, a component of the TREX-2 complex involved in coupling of nuclear mRNA export with transcription, as multi-copy suppressors for the ptr5–1 mutation. In addition, we found that the ptr5–1 mutation is synthetically lethal with a mutation of the mRNA export factor Rae1p, and that the double mutant exaggerates defective nuclear mRNA export, suggesting that Ptr5p/Nup85p is involved in nuclear mRNA export through Rae1p. Interestingly, the ptr5–1 mutation also showed synthetic effects with several prp pre-mRNA splicing mutations, suggesting a functional linkage between the NPCs and the splicing apparatus in the yeast nucleus.

  7. Influenza polymerase encoding mRNAs utilize atypical mRNA nuclear export.

    Science.gov (United States)

    Larsen, Sean; Bui, Steven; Perez, Veronica; Mohammad, Adeba; Medina-Ramirez, Hilario; Newcomb, Laura L

    2014-08-28

    Influenza is a segmented negative strand RNA virus. Each RNA segment is encapsulated by influenza nucleoprotein and bound by the viral RNA dependent RNA polymerase (RdRP) to form viral ribonucleoproteins responsible for RNA synthesis in the nucleus of the host cell. Influenza transcription results in spliced mRNAs (M2 and NS2), intron-containing mRNAs (M1 and NS1), and intron-less mRNAs (HA, NA, NP, PB1, PB2, and PA), all of which undergo nuclear export into the cytoplasm for translation. Most cellular mRNA nuclear export is Nxf1-mediated, while select mRNAs utilize Crm1. Here we inhibited Nxf1 and Crm1 nuclear export prior to infection with influenza A/Udorn/307/1972(H3N2) virus and analyzed influenza intron-less mRNAs using cellular fractionation and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). We examined direct interaction between Nxf1 and influenza intron-less mRNAs using immuno purification of Nxf1 and RT-PCR of associated RNA. Inhibition of Nxf1 resulted in less influenza intron-less mRNA export into the cytoplasm for HA and NA influenza mRNAs in both human embryonic kidney cell line (293 T) and human lung adenocarcinoma epithelial cell line (A549). However, in 293 T cells no change was observed for mRNAs encoding the components of the viral ribonucleoproteins; NP, PA, PB1, and PB2, while in A549 cells, only PA, PB1, and PB2 mRNAs, encoding the RdRP, remained unaffected; NP mRNA was reduced in the cytoplasm. In A549 cells NP, NA, HA, mRNAs were found associated with Nxf1 but PA, PB1, and PB2 mRNAs were not. Crm1 inhibition also resulted in no significant difference in PA, PB1, and PB2 mRNA nuclear export. These results further confirm Nxf1-mediated nuclear export is functional during the influenza life cycle and hijacked for select influenza mRNA nuclear export. We reveal a cell type difference for Nxf1-mediated nuclear export of influenza NP mRNA, a reminder that cell type can influence molecular mechanisms. Importantly, we

  8. Safety surveillance of activities on nuclear pressure components in China

    International Nuclear Information System (INIS)

    Li Ganjie; Li Tianshu; Yan Tianwen

    2005-01-01

    The nuclear pressure components, which perform the nuclear safety functions, are one of the key physical barriers for nuclear safety. For the national strategy on further development of nuclear power and localization of nuclear pressure components, there still exist some problems in preparedness on the localization. As for the technical basis, what can not be overlooked is the management. Aiming at the current problems, National Nuclear Safety Administration (NNSA) has taken measures to strengthen the propagation and popularization of nuclear safety culture, adjust the review and approval policies for nuclear pressure components qualification license, establish more stringent management requirements, and enhance the surveillance of activities on nuclear pressure equipment. Meanwhile, NNSA has improved the internal management and the regulation efficiency on nuclear pressure components. At the same time, with the development and implementation of 'Rules on the Safety Regulation for Nuclear Safety Important Components' to be promulgated by the State Council of China, NNSA will complete and improve the regulation on nuclear pressure components and other nuclear equipment. (authors)

  9. The actin binding cytoskeletal protein Moesin is involved in nuclear mRNA export.

    Science.gov (United States)

    Kristó, Ildikó; Bajusz, Csaba; Borsos, Barbara N; Pankotai, Tibor; Dopie, Joseph; Jankovics, Ferenc; Vartiainen, Maria K; Erdélyi, Miklós; Vilmos, Péter

    2017-10-01

    Current models imply that the evolutionarily conserved, actin-binding Ezrin-Radixin-Moesin (ERM) proteins perform their activities at the plasma membrane by anchoring membrane proteins to the cortical actin network. Here we show that beside its cytoplasmic functions, the single ERM protein of Drosophila, Moesin, has a novel role in the nucleus. The activation of transcription by heat shock or hormonal treatment increases the amount of nuclear Moesin, indicating biological function for the protein in the nucleus. The distribution of Moesin in the nucleus suggests a function in transcription and the depletion of mRNA export factors Nup98 or its interacting partner, Rae1, leads to the nuclear accumulation of Moesin, suggesting that the nuclear function of the protein is linked to mRNA export. Moesin localizes to mRNP particles through the interaction with the mRNA export factor PCID2 and knock down of Moesin leads to the accumulation of mRNA in the nucleus. Based on our results we propose that, beyond its well-known, manifold functions in the cytoplasm, the ERM protein of Drosophila is a new, functional component of the nucleus where it participates in mRNA export. Copyright © 2017 Elsevier B.V. All rights reserved.

  10. Inner nuclear envelope protein SUN1 plays a prominent role in mammalian mRNA export.

    Science.gov (United States)

    Li, Ping; Noegel, Angelika A

    2015-11-16

    Nuclear export of messenger ribonucleoproteins (mRNPs) through the nuclear pore complex (NPC) can be roughly classified into two forms: bulk and specific export, involving an nuclear RNA export factor 1 (NXF1)-dependent pathway and chromosome region maintenance 1 (CRM1)-dependent pathway, respectively. SUN proteins constitute the inner nuclear envelope component of the l I: nker of N: ucleoskeleton and C: ytoskeleton (LINC) complex. Here, we show that mammalian cells require SUN1 for efficient nuclear mRNP export. The results indicate that both SUN1 and SUN2 interact with heterogeneous nuclear ribonucleoprotein (hnRNP) F/H and hnRNP K/J. SUN1 depletion inhibits the mRNP export, with accumulations of both hnRNPs and poly(A)+RNA in the nucleus. Leptomycin B treatment indicates that SUN1 functions in mammalian mRNA export involving the NXF1-dependent pathway. SUN1 mediates mRNA export through its association with mRNP complexes via a direct interaction with NXF1. Additionally, SUN1 associates with the NPC through a direct interaction with Nup153, a nuclear pore component involved in mRNA export. Taken together, our results reveal that the inner nuclear envelope protein SUN1 has additional functions aside from being a central component of the LINC complex and that it is an integral component of the mammalian mRNA export pathway suggesting a model whereby SUN1 recruits NXF1-containing mRNP onto the nuclear envelope and hands it over to Nup153. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

  11. Postage for the messenger: Designating routes for Nuclear mRNA Export

    Science.gov (United States)

    Natalizio, Barbara J.; Wente, Susan R.

    2013-01-01

    Transcription of messenger(m) RNA occurs in the nucleus, making the translocation of mRNA across the nuclear envelope (NE) boundary a critical determinant of proper gene expression and cell survival. A major mRNA export route occurs via the NXF1-dependent pathway through the nuclear pore complexes (NPCs) embedded in the NE. However, recent findings have discovered new evidence supporting the existence of multiple mechanisms for crossing the NE, including both NPC-mediated and NE budding-mediated pathways. An analysis of the trans-acting factors and cis components that define these pathways reveals shared elements as well as mechanistic differences. We review here the current understanding of the mechanisms that characterize each pathway and highlight the determinants that influence mRNA transport fate. PMID:23583578

  12. Rift Valley fever virus NSS gene expression correlates with a defect in nuclear mRNA export.

    Science.gov (United States)

    Copeland, Anna Maria; Van Deusen, Nicole M; Schmaljohn, Connie S

    2015-12-01

    We investigated the localization of host mRNA during Rift Valley fever virus (RVFV) infection. Fluorescence in situ hybridization revealed that infection with RVFV altered the localization of host mRNA. mRNA accumulated in the nuclei of RVFV-infected but not mock-infected cells. Further, overexpression of the NSS gene, but not the N, GN or NSM genes correlated with mRNA nuclear accumulation. Nuclear accumulation of host mRNA was not observed in cells infected with a strain of RVFV lacking the gene encoding NSS, confirming that expression of NSS is likely responsible for this phenomenon. Published by Elsevier Inc.

  13. Nuclear power stations: environmental surveillance of radioactivity

    International Nuclear Information System (INIS)

    Pellerin, P.

    1972-01-01

    Because of the radiations they emit, radioactive substances can be detected, identified and measured at extremely low concentrations ? the corresponding masses are lower by a factor ranging from 1000 to 10 000 than those that can be measured by any other chemical or physical method, however precise, applied to non-radioactive substances. Radioisotopes can therefore be detected in the environment at levels much lower than those at which genuine public health problems begin to arise. Unfortunately, we cannot say the same of numerous non-radio active pollutants, which can be measured only at concentrations very close to, or even exceeding, the toxicity threshold. In the mind of the uninformed public confusion seems quite frequently to reign as between the detection threshold and the toxicity threshold. This undoubtedly explains the following situation which is, to say the least, paradoxical: people are afraid of the hypothetical effects of radioactivity at ridiculously low levels, whereas nobody is alarmed at the fact that the toxicity limits for a very large number of non-radioactive, but very real pollutants are being exceeded almost continuously. The sum of all artificial irradiations does not exceed the normal fluctuations of natural irradiation, and if the genetic effects of very low radiation doses were truly cumulative, the natural radiation to which we are all exposed and which is by far the highest would by itself have eliminated every trace of life on earth long ago. Lastly, let us not forget that merely the use of X-rays in medicine, particularly in radiodiagnosis, represents an additional average artificial irradiation of the population amounting to double the natural radiation (100 millirem per year). This is about 100 times the irradiation which would accrue from nuclear industry even according to the most pessimistic estimate. We have seen that the measures described above will make it genuinely possible to maintain environmental radioactivity in all

  14. Magnetic signature surveillance of nuclear fuel

    International Nuclear Information System (INIS)

    Bernatowicz, H.; Schoenig, F.C.

    1981-01-01

    Typical nuclear fuel material contains tramp ferromagnetic particles of random size and distribution. Also, selected amounts of paramagnetic or ferromagnetic material can be added at random or at known positions in the fuel material. The fuel material in its non-magnetic container is scanned along its length by magnetic susceptibility detecting apparatus whereby susceptibility changes along its length are obtained and provide a unique signal waveform of the container of fuel material as a signature thereof. The output signature is stored. At subsequent times in its life the container is again scanned and respective signatures obtained which are compared with the initially obtained signature, any differences indicating alteration or tampering with the fuel material. If the fuel material includes a paramagnetic additive by taking two measurements along the container the effects thereof can be cancelled out. (author)

  15. Methods for surveillance of noise signals from nuclear power plants using auto power spectra

    International Nuclear Information System (INIS)

    Streich, M.

    1988-01-01

    A survey of methods for noise diagnostics applied in the nuclear power plant 'Bruno Leuschner' for surveillance of primary circuit is given. Considering a special example concept of surveillance of standard deviations is explained. (author)

  16. Radiological surveillance in the nuclear fuel fabrication in Mexico

    International Nuclear Information System (INIS)

    Garcia A, J.; Reynoso V, R.; Delgado A, G.

    1996-01-01

    The objective of this report is to present the obtained results related to the application of the radiological safety programme established at the Nuclear Fuel Fabrication Pilot Plant (NFFPF) in Mexico, such as: surveillance methods, radiological protection criteria and regulations, radiation control and records and the application of ALARA recommendation. During the starting period from April 1994 to April 1995, at the NFFPF were made two nuclear fuel bundles a Dummy and other to be burned up in a BWR the mainly process activities are: UO 2 powder receiving, powder pressing for the pellets formation, pellets grinding, cleaning and drying, loading into a rod, Quality Control testing, nuclear fuel bundles assembly. The NFFPF is divided into an unsealed source area (pellets manufacturing plant) and into a sealed source area (rods fabrication plant). The control followed have helped to detect failures and to improve the safety programme and operation. (authors). 1 ref., 3 figs

  17. Surveillance of items important to safety in nuclear power plants

    International Nuclear Information System (INIS)

    1990-01-01

    The Guide was prepared as part of the IAEA's programme, referred to as the NUSS Programme, for establishing Codes and Safety Guides relating to nuclear power plants. THe Guide supplements the Code on the Safety of Nuclear Power Plants: Operation, IAEA Safety Series No. 50-C-O(Rev.1). The operating organization has overall responsibility for the safe operation of the nuclear power plant. Therefore, it shall ensure that adequate surveillance activities are carried out in order to verify that the plant is operated within the prescribed operational limits and conditions, and to detect in time any deterioration of structures, systems and components as well as any adverse trend that could lead to an unsafe condition. These activities can be classified as: Monitoring plant parameters and system status; Checking and calibrating instrumentation; Testing and inspecting structures, systems and components. This Safety Guide provides guidance and recommendations on surveillance activities to ensure that structures, systems and components important to safety are available to perform their functions in accordance with design intent and assumptions

  18. Preparation of Act on State Surveillance of Nuclear Safety of Nuclear Installations

    International Nuclear Information System (INIS)

    Kyncl, J.

    1983-01-01

    The Czechoslovak Government Decree no. 179 of June 1982 approved the principles underlying the first Czechoslovak legal norm to complexly resolve the problem of State surveillance of nuclear safety of nuclear installations. In the introduction the law will define the concept of nuclear safety of nuclear installations and will justify the reasons for which it has to be assured. The individual parts of the law will deal with the establishment of State surveillance of nuclear safety, the tasks of the Czechoslovak Atomic Energy Commission in this area, the control activity of Commission personnel, the measures taken against responsible organizations and personnel for failing to observe their duties, the obligations of bodies and organizations, and the cooperation between inspection bodies. (A.K.)

  19. Fuzzy operation and real time surveillance of a nuclear reactor

    International Nuclear Information System (INIS)

    Si-Fodil, M.; Guely, F.; Siarry, P.; Tyran, J.L.

    1997-01-01

    The operating power of nuclear power plants needs to be modulated according to the thin evolutions of electric power demand. Two parameters are concerned by load following operations: the power axial disequilibrium and the position of control rods. This paper deals with the automation of the control of power axial disequilibrium using boration-dilution. An automatic system based on fuzzy logic is proposed which can be substituted to the expert operator who is in charge of this fastidious manual task. The management of water and boron flow rates are studied in details. A Graphic interface was designed for the real-time surveillance of the reactor. (J.S.)

  20. Radiation protection in nuclear facilities. The Caise environmental surveillance system

    International Nuclear Information System (INIS)

    Witt, H. de; Voelz, E.

    1995-01-01

    The Computer Aided Surveillance System for the Environment of Nuclear Installations (Caise) has been designed for permanent surveillance of the environment of nuclear installations under normal operating conditions and for unusual events on the basis of radiological and meteorological measured data. In normal operation, the data measured on line are fed to the system by way of a defined interface, while off-line data can be entered manually in the dialog mode. Subsequently, the measured data are stored, filed away, and secured. Short-time dispersion factors can be calculated permanently in the on-line mode, while the off-line mode allows short-term and long-term dispersions to be calculated for randomly selectable periods of time under the General Administrative Rule of Sec. 45 of the German Radiation Protection Ordinance. The corresponding dose distributions in the environment of the plant can be determined next. Under conditions of increased emissions (accidents, failures), Caise assists in quick decision-making by its capacity for real-time dispersion calculations including current on-line and off-line emission sample measurements. In this way, the contributions by various different exposure pathways to the calculated dose can be determined more accurately. (orig.) [de

  1. High-Resolution Imaging Reveals New Features of Nuclear Export of mRNA through the Nuclear Pore Complexes

    Directory of Open Access Journals (Sweden)

    Joseph M. Kelich

    2014-08-01

    Full Text Available The nuclear envelope (NE of eukaryotic cells provides a physical barrier for messenger RNA (mRNA and the associated proteins (mRNPs traveling from sites of transcription in the nucleus to locations of translation processing in the cytoplasm. Nuclear pore complexes (NPCs embedded in the NE serve as a dominant gateway for nuclear export of mRNA. However, the fundamental characterization of export dynamics of mRNPs through the NPC has been hindered by several technical limits. First, the size of NPC that is barely below the diffraction limit of conventional light microscopy requires a super-resolution microscopy imaging approach. Next, the fast transit of mRNPs through the NPC further demands a high temporal resolution by the imaging approach. Finally, the inherent three-dimensional (3D movements of mRNPs through the NPC demand the method to provide a 3D mapping of both transport kinetics and transport pathways of mRNPs. This review will highlight the recently developed super-resolution imaging techniques advanced from 1D to 3D for nuclear export of mRNPs and summarize the new features in the dynamic nuclear export process of mRNPs revealed from these technical advances.

  2. Independent body for surveillance over nuclear safety in the Slovak Republic established

    International Nuclear Information System (INIS)

    Zlatnansky, J.

    1994-01-01

    The position, role, tasks and organizational structure of the Nuclear Safety Authority of the Slovak Republic are outlined. The Authority is responsible for state surveillance over nuclear safety of nuclear facilities including surveillance over radioactive waste management, over spent fuel management and other stages of the fuel cycle, as well as over nuclear materials including their accountancy and recording. The body is also responsible for assessing projects within the nuclear energy use programme and the quality of selected nuclear technological facilities and instrumentation, as well as for Slovak commitments and obligations under international agreements concerned with nuclear safety of nuclear facilities and with radioactive wastes. (J.B.). 1 fig

  3. Advanced surveillance of Resistance Temperature Detectors in Nuclear Power Plants

    International Nuclear Information System (INIS)

    Montalvo, C.; García-Berrocal, A.; Bermejo, J.A.; Queral, C.

    2014-01-01

    Highlights: • A two time constant transfer function is proposed to describe the Resistance Temperature Detector dynamics. • One constant is only related to the inner dynamics whereas the other one is related to the process and to the inner dynamics. • The two time constants have been found in several RTDs from a Nuclear Power Plant. • A Monte Carlo simulation is used to properly adjust the sampling time to find both constants. - Abstract: The dynamic response of several RTDs located at the cold leg of a PWR has been studied. A theoretical model for the heat transfer between the RTDs and the surrounding fluid is derived. It proposes a two real poles transfer function. By means of noise analysis techniques in the time domain (autoregressive models) and the Dynamic Data System methodology, the two time constants of the system can be found. A Monte Carlo simulation is performed in order to choose the proper sampling time to obtain both constants. The two poles are found and they permit an advance in situ surveillance of the sensor response time and the sensor dynamics performance. One of the poles is related to the inner dynamics whereas the other one is linked to the process and the inner dynamics. So surveillance on the process and on the inner dynamics can be distinguished

  4. Nuclear material safeguards surveillance and accountancy by isotope correlation techniques

    International Nuclear Information System (INIS)

    Persiani, P.J.; Goleb, J.A.; Kroc, T.K.

    1981-11-01

    The purpose of this study is to investigate the applicability of isotope correlation techniques (ICT) to the Light Water Reactor (LWR) and the Liquid Metal Fast Breeder Reactor (LMFBR) fuel cycles for nuclear material accountancy and safeguards surveillance. The isotopic measurement of the inventory input to the reprocessing phase of the fuel cycle is the primary direct determination that an anomaly may exist in the fuel management of nuclear material. The nuclear materials accountancy gap which exists between the fabrication plant output and the input to the reprocessing plant can be minimized by using ICT at the dissolver stage of the reprocessing plant. The ICT allows a level of verification of the fabricator's fuel content specifications, the irradiation history, the fuel and blanket assemblies management and scheduling within the reactor, and the subsequent spent fuel assembly flows to the reprocessing plant. The investigation indicates that there exist relationships between isotopic concentration which have predictable, functional behavior over a range of burnup. Several cross-correlations serve to establish the initial core assembly-averaged composition. The selection of the more effective functionals will depend not only on the level of reliability of ICT for verification, but also on the capability, accuracy and difficulty of developing measurement methods. The propagation of measurement errors on the correlation functions and respective sensitivities to isotopic compositional changes have been examined and found to be consistent with current measurement methods

  5. Nuclear imprisonment of host cellular mRNA by nsp1β protein of porcine reproductive and respiratory syndrome virus

    International Nuclear Information System (INIS)

    Han, Mingyuan; Ke, Hanzhong; Zhang, Qingzhan; Yoo, Dongwan

    2017-01-01

    Positive-strand RNA genomes function as mRNA for viral protein synthesis which is fully reliant on host cell translation machinery. Competing with cellular protein translation apparatus needs to ensure the production of viral proteins, but this also stifles host innate defense. In the present study, we showed that porcine reproductive and respiratory syndrome virus (PRRSV), whose replication takes place in the cytoplasm, imprisoned host cell mRNA in the nucleus, which suggests a novel mechanism to enhance translation of PRRSV genome. PRRSV nonstructural protein (nsp) 1β was identified as the nuclear protein playing the role for host mRNA nuclear retention and subversion of host protein synthesis. A SAP (SAF-A/B, Acinus, and PIAS) motif was identified in nsp1β with the consensus sequence of 126 -LQxxLxxxGL- 135 . In situ hybridization unveiled that SAP mutants were unable to cause nuclear retention of host cell mRNAs and did not suppress host protein synthesis. In addition, these SAP mutants reverted PRRSV-nsp1β-mediated suppression of interferon (IFN) production, IFN signaling, and TNF-α production pathway. Using reverse genetics, a series of SAP mutant PRRS viruses, vK124A, vL126A, vG134A, and vL135A were generated. No mRNA nuclear retention was observed during vL126A and vL135A infections. Importantly, vL126A and vL135A did not suppress IFN production. For other arteriviruses, mRNA nuclear accumulation was also observed for LDV-nsp1β and SHFV-nsp1β. EAV-nsp1 was exceptional and did not block the host mRNA nuclear export. - Highlights: •PRRS virus blocks host mRNA nuclear export to the cytoplasm. •PRRSV nsp1β is the viral protein responsible for host mRNA nuclear retention. •SAP domain in nsp1β is essential for host mRNA nuclear retention and type I interferon suppression. •Mutation in the SAP domain of nsp1β causes the loss of function. •Host mRNA nuclear retention by nsp1β is common in the family Arteriviridae, except equine arteritis virus.

  6. Nuclear imprisonment of host cellular mRNA by nsp1β protein of porcine reproductive and respiratory syndrome virus

    Energy Technology Data Exchange (ETDEWEB)

    Han, Mingyuan, E-mail: hanming@umich.edu; Ke, Hanzhong; Zhang, Qingzhan; Yoo, Dongwan, E-mail: dyoo@illinois.edu

    2017-05-15

    Positive-strand RNA genomes function as mRNA for viral protein synthesis which is fully reliant on host cell translation machinery. Competing with cellular protein translation apparatus needs to ensure the production of viral proteins, but this also stifles host innate defense. In the present study, we showed that porcine reproductive and respiratory syndrome virus (PRRSV), whose replication takes place in the cytoplasm, imprisoned host cell mRNA in the nucleus, which suggests a novel mechanism to enhance translation of PRRSV genome. PRRSV nonstructural protein (nsp) 1β was identified as the nuclear protein playing the role for host mRNA nuclear retention and subversion of host protein synthesis. A SAP (SAF-A/B, Acinus, and PIAS) motif was identified in nsp1β with the consensus sequence of {sub 126}-LQxxLxxxGL-{sub 135}. In situ hybridization unveiled that SAP mutants were unable to cause nuclear retention of host cell mRNAs and did not suppress host protein synthesis. In addition, these SAP mutants reverted PRRSV-nsp1β-mediated suppression of interferon (IFN) production, IFN signaling, and TNF-α production pathway. Using reverse genetics, a series of SAP mutant PRRS viruses, vK124A, vL126A, vG134A, and vL135A were generated. No mRNA nuclear retention was observed during vL126A and vL135A infections. Importantly, vL126A and vL135A did not suppress IFN production. For other arteriviruses, mRNA nuclear accumulation was also observed for LDV-nsp1β and SHFV-nsp1β. EAV-nsp1 was exceptional and did not block the host mRNA nuclear export. - Highlights: •PRRS virus blocks host mRNA nuclear export to the cytoplasm. •PRRSV nsp1β is the viral protein responsible for host mRNA nuclear retention. •SAP domain in nsp1β is essential for host mRNA nuclear retention and type I interferon suppression. •Mutation in the SAP domain of nsp1β causes the loss of function. •Host mRNA nuclear retention by nsp1β is common in the family Arteriviridae, except equine

  7. Nuclear disarmament. Options for the coming non-proliferation treaty surveillance cycle

    International Nuclear Information System (INIS)

    Mueller, Harald

    2011-01-01

    The report is aimed on the nuclear disarmament discussion with respect to the disagreement of nuclear weapon states and those without nuclear weapons, esp. the non-aligned movement (NAM) concerning the non-proliferation treaty. The report covers the following issues: The role of the non-proliferation treaty, nuclear disarmament in the last surveillance conference 2010, the different disarmament philosophies, the possibilities of bridging the disagreement, further disarmament options for the future non-proliferation treaty surveillance cycle, German options for the future surveillance cycle.

  8. Surveillance

    DEFF Research Database (Denmark)

    Albrechtslund, Anders; Coeckelbergh, Mark; Matzner, Tobias

    Studying surveillance involves raising questions about the very nature of concepts such as information, technology, identity, space and power. Besides the maybe all too obvious ethical issues often discussed with regard to surveillance, there are several other angles and approaches that we should...... like to encourage. Therefore, our panel will focus on the philosophical, yet non-ethical issues of surveillance in order to stimulate an intense debate with the audience on the ethical implications of our enquiries. We also hope to provide a broader and deeper understanding of surveillance....

  9. Rift Valley fever virus NS{sub S} gene expression correlates with a defect in nuclear mRNA export

    Energy Technology Data Exchange (ETDEWEB)

    Copeland, Anna Maria; Van Deusen, Nicole M.; Schmaljohn, Connie S., E-mail: Connie.s.schmaljohn.civ@mail.mil

    2015-12-15

    We investigated the localization of host mRNA during Rift Valley fever virus (RVFV) infection. Fluorescence in situ hybridization revealed that infection with RVFV altered the localization of host mRNA. mRNA accumulated in the nuclei of RVFV-infected but not mock-infected cells. Further, overexpression of the NS{sub S} gene, but not the N, G{sub N} or NS{sub M} genes correlated with mRNA nuclear accumulation. Nuclear accumulation of host mRNA was not observed in cells infected with a strain of RVFV lacking the gene encoding NS{sub S}, confirming that expression of NS{sub S} is likely responsible for this phenomenon. - Highlights: • Rift Valley fever virus (RVFV) infection alters the localization of host mRNA. • mRNA accumulates in the nuclei of RVFV-infected but not mock-infected cells. • NS{sub S} is likely responsible for mRNA relocalization to the nucleus.

  10. A biochemical framework for eIF4E-dependent mRNA export and nuclear recycling of the export machinery.

    Science.gov (United States)

    Volpon, Laurent; Culjkovic-Kraljacic, Biljana; Sohn, Hye Seon; Blanchet-Cohen, Alexis; Osborne, Michael J; Borden, Katherine L B

    2017-06-01

    The eukaryotic translation initiation factor eIF4E acts in the nuclear export and translation of a subset of mRNAs. Both of these functions contribute to its oncogenic potential. While the biochemical mechanisms that underlie translation are relatively well understood, the molecular basis for eIF4E's role in mRNA export remains largely unexplored. To date, over 3000 transcripts, many encoding oncoproteins, were identified as potential nuclear eIF4E export targets. These target RNAs typically contain a ∼50-nucleotide eIF4E sensitivity element (4ESE) in the 3' UTR and a 7-methylguanosine cap on the 5' end. While eIF4E associates with the cap, an unknown factor recognizes the 4ESE element. We previously identified cofactors that functionally interacted with eIF4E in mammalian cell nuclei including the leucine-rich pentatricopeptide repeat protein LRPPRC and the export receptor CRM1/XPO1. LRPPRC simultaneously interacts with both eIF4E bound to the 5' mRNA cap and the 4ESE element in the 3' UTR. In this way, LRPPRC serves as a specificity factor to recruit 4ESE-containing RNAs within the nucleus. Further, we show that CRM1 directly binds LRPPRC likely acting as the export receptor for the LRPPRC-eIF4E-4ESE RNA complex. We also found that Importin 8, the nuclear importer for cap-free eIF4E, imports RNA-free LRPPRC, potentially providing both coordinated nuclear recycling of the export machinery and an important surveillance mechanism to prevent futile export cycles. Our studies provide the first biochemical framework for the eIF4E-dependent mRNA export pathway. © 2017 Volpon et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.

  11. Intelligent robots for nuclear power plant inspection and surveillance

    International Nuclear Information System (INIS)

    Miyazawa, Tatsuo; Suzuki, Kazumi; Fujie, Hideo; Fujii, Masaaki; Asai, Takashi; Sugimoto, Hiroshi.

    1986-01-01

    Recently, the research and development of robotizing the patrol and works in nuclear power plants have been actively carried out since the TMI-2 accident in March, 1979. In this paper, among these robots, six examples of the movable robots, of which the working and movement were intellectualized by using information processing techniques and others, are reported, and their intellectualization is concretely discussed. In Japan, the development of the supporting system for nuclear power generation was carried out for five years from fiscal year 1980 as the project subsidized by the Ministry of International Trade and Industry, and during this period, the inspection robots for LWR plants were developed. The development of the robots for ultimate working as the large scale project of the Agency of Industrial Science and Technology aiming at further heightening the function is in progress as the eight-year project from fiscal year 1983. Monorail type automatic surveillance robots, system maintenance robots 'AMOOTY', variable crawler type intelligent movable robots, hybrid running type intelligent movable robots, monorail running type small checkup robots, and floor running type checkup and light work robots are reported. Sense information processing control and a robot language processor for expanding the function of autonomous control are outlined. (Kako, I.)

  12. Characterization of the ptr5{sup +} gene involved in nuclear mRNA export in fission yeast

    Energy Technology Data Exchange (ETDEWEB)

    Watanabe, Nobuyoshi; Ikeda, Terumasa; Mizuki, Fumitaka [Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kurokami, Kumamoto 860-8555 (Japan); Tani, Tokio, E-mail: ttani@sci.kumamoto-u.ac.jp [Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kurokami, Kumamoto 860-8555 (Japan)

    2012-02-03

    Highlights: Black-Right-Pointing-Pointer We cloned the ptr5{sup +} gene involved in nuclear mRNA export in fission yeast. Black-Right-Pointing-Pointer The ptr5{sup +} gene was found to encode nucleoporin 85 (Nup85). Black-Right-Pointing-Pointer Seh1p and Mlo3p are multi-copy suppressors for the ptr5 mutation. Black-Right-Pointing-Pointer Ptr5p/Nup85p functions in nuclear mRNA export through the mRNA export factor Rae1p. Black-Right-Pointing-Pointer Ptr5p/Nup85p interacts genetically with pre-mRNA splicing factors. -- Abstract: To analyze the mechanisms of mRNA export from the nucleus to the cytoplasm, we have isolated eleven mutants, ptr [poly(A){sup +} RNA transport] 1 to 11, which accumulate poly(A){sup +} RNA in the nucleus at a nonpermissive temperature in Schizosaccharomyces pombe. Of those, the ptr5-1 mutant shows dots- or a ring-like accumulation of poly(A){sup +} RNA at the nuclear periphery after shifting to the nonpermissive temperature. We cloned the ptr5{sup +} gene and found that it encodes a component of the nuclear pore complex (NPC), nucleoporin 85 (Nup85). The ptr5-1 mutant shows no defects in protein transport, suggesting the specific involvement of Ptr5p/Nup85p in nuclear mRNA export in S. pombe. We identified Seh1p, a nucleoporin interacting with Nup85p, an mRNA-binding protein Mlo3p, and Sac3p, a component of the TREX-2 complex involved in coupling of nuclear mRNA export with transcription, as multi-copy suppressors for the ptr5-1 mutation. In addition, we found that the ptr5-1 mutation is synthetically lethal with a mutation of the mRNA export factor Rae1p, and that the double mutant exaggerates defective nuclear mRNA export, suggesting that Ptr5p/Nup85p is involved in nuclear mRNA export through Rae1p. Interestingly, the ptr5-1 mutation also showed synthetic effects with several prp pre-mRNA splicing mutations, suggesting a functional linkage between the NPCs and the splicing apparatus in the yeast nucleus.

  13. Surveillance strategy for an extended operating cycle in commercial nuclear reactors

    International Nuclear Information System (INIS)

    McHenry, R.S.; Moore, T.J.; Maurer, J.H.; Todreas, N.E.

    1997-01-01

    The impetus for improved economic performance of commercial nuclear power plants can be partially satisfied by increasing plant capacity factors through operating cycle extension. One aspect of an operating cycle extension effort is the modification of plant surveillance programs to complete required regulatory and investment protection surveillance activities within the extended planned outage schedule. The goal is to introduce a general strategy for existing power plants to transition their surveillance programs to an extended operating cycle up to 48 months in length, and to test the feasibility of this strategy through the complete analysis of the surveillance programs at operating BWR and PWR case study plants. The reconciliation of surveillances at these plants demonstrates that surveillance performance will not preclude 48 month operating cycles. Those surveillance activities that could not be resolved to an extended cycle are identified for further study. Finally, a number of general issues are presented that should be considered before implementing a cycle extension effort

  14. Surveillance strategy for an extended operating cycle in commercial nuclear reactors

    International Nuclear Information System (INIS)

    McHenry, R.S.; Moore, T.J.; Maurer, J.H.; Todreas, N.E.

    1997-01-01

    The impetus for improved economic performance of commercial nuclear power plants can be partially satisfied by increasing plant capacity factors through operating cycle extension. One aspect of an operating cycle extension effort is the modification of plant surveillance programs to complete required regulatory and investment protection surveillance activities within the extended planned outage schedule. The goal of this paper is to introduce a general strategy for existing power plants to transition their surveillance programs to an extended operating cycle up to 48 months in length, and to test the feasibility of this strategy through the complete analysis of the surveillance programs at operating BWR and PWR case study plants. The reconciliation of surveillances at these plants demonstrates that surveillance performance will not preclude 48 month operating cycles. Those surveillance activities that could not be resolved to an extended cycle are identified for further study. Finally, a number of general issues are presented that should be considered before implementing a cycle extension effort

  15. Environmental hardening of robots for nuclear maintenance and surveillance tasks

    International Nuclear Information System (INIS)

    Hintenlang, D.E.; Tulenko, J.S.; Wheeler, R.; Roy, T.

    1990-01-01

    The University of Florida, in cooperation with the Universities of Texas, Tennessee, and Michigan and Oak Ridge National Laboratory, is developing an advanced robotic system for the US Department of Energy under the University Program for Robotics for Advanced Reactors. As part of this program, the University of Florida has been pursuing the development of environmentally hardened components so that autonomous robotic systems can successfully carry out their tasks under the most extreme expected environmental conditions. This requirement means that the designed robotic system with its onboard computer-based intelligence must be able to successfully complete tasks in toxic, radioactive, wet, temperature extremes, and other physically impairing environments. As part of this program, a study was carried out to determine the environmental conditions that should be set as the design criteria for robotic systems to maintain reasonable operations for nuclear plants in the course of maintenance, testing, and surveillance under all conditions, including plant upset. It was decided that Florida would build a combined environmental testing facility to test specific devices in high-radiation/high-temperature combined environments. This environmental test chamber has been built and successfully tested to over 250 degree F. This facility will provide some of the first combined temperatures/radiation data for many large-scale integrated components

  16. Surveillance of instrumentation channels at nuclear power plants

    International Nuclear Information System (INIS)

    Thie, J.A.

    1989-06-01

    Surveillance activities at nuclear plants, involving-calibrations, functional tests, and simple checks, have many associated problems. These problems are of the following four types: administrative, equipment, human, and systemic. An extensive search of the literature has led to 63 generic classes of problems falling within these types and involving instrumentation department activities. The classification system is that which writers of incidents are essentially using, based on historical traditions, in naming their problems' causes. An interesting finding in the search was the strong correlation of this project to many aspects of industrial safety; technology transfer opportunities from the latter are identified. A survey of plant instrumentation experts was conducted to obtain a ranking of the most important problems. Classes of solutions to these problems are listed and discussed. Outlined is a possible methodology of matching these solutions to problems. Finally applications of this study are listed, and include extensions to training, operations, and maintenance departments of power plants. Appendices give several general examples for each problem class and many specific suggestions from experts on addressing the problems felt to be more important. 15 refs., 2 figs., 6 tabs

  17. Influenza A Virus NS1 Protein Promotes Efficient Nuclear Export of Unspliced Viral M1 mRNA.

    Science.gov (United States)

    Pereira, Carina F; Read, Eliot K C; Wise, Helen M; Amorim, Maria J; Digard, Paul

    2017-08-01

    Influenza A virus mRNAs are transcribed by the viral RNA-dependent RNA polymerase in the cell nucleus before being exported to the cytoplasm for translation. Segment 7 produces two major transcripts: an unspliced mRNA that encodes the M1 matrix protein and a spliced transcript that encodes the M2 ion channel. Export of both mRNAs is dependent on the cellular NXF1/TAP pathway, but it is unclear how they are recruited to the export machinery or how the intron-containing but unspliced M1 mRNA bypasses the normal quality-control checkpoints. Using fluorescent in situ hybridization to monitor segment 7 mRNA localization, we found that cytoplasmic accumulation of unspliced M1 mRNA was inefficient in the absence of NS1, both in the context of segment 7 RNPs reconstituted by plasmid transfection and in mutant virus-infected cells. This effect was independent of any major effect on steady-state levels of segment 7 mRNA or splicing but corresponded to a ∼5-fold reduction in the accumulation of M1. A similar defect in intronless hemagglutinin (HA) mRNA nuclear export was seen with an NS1 mutant virus. Efficient export of M1 mRNA required both an intact NS1 RNA-binding domain and effector domain. Furthermore, while wild-type NS1 interacted with cellular NXF1 and also increased the interaction of segment 7 mRNA with NXF1, mutant NS1 polypeptides unable to promote mRNA export did neither. Thus, we propose that NS1 facilitates late viral gene expression by acting as an adaptor between viral mRNAs and the cellular nuclear export machinery to promote their nuclear export. IMPORTANCE Influenza A virus is a major pathogen of a wide variety of mammalian and avian species that threatens public health and food security. A fuller understanding of the virus life cycle is important to aid control strategies. The virus has a small genome that encodes relatively few proteins that are often multifunctional. Here, we characterize a new function for the NS1 protein, showing that, as well as

  18. The function of the inner nuclear envelope protein SUN1 in mRNA export is regulated by phosphorylation.

    Science.gov (United States)

    Li, Ping; Stumpf, Maria; Müller, Rolf; Eichinger, Ludwig; Glöckner, Gernot; Noegel, Angelika A

    2017-08-22

    SUN1, a component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, functions in mammalian mRNA export through the NXF1-dependent pathway. It associates with mRNP complexes by direct interaction with NXF1. It also binds to the NPC through association with the nuclear pore component Nup153, which is involved in mRNA export. The SUN1-NXF1 association is at least partly regulated by a protein kinase C (PKC) which phosphorylates serine 113 (S113) in the N-terminal domain leading to reduced interaction. The phosphorylation appears to be important for the SUN1 function in nuclear mRNA export since GFP-SUN1 carrying a S113A mutation was less efficient in restoring mRNA export after SUN1 knockdown as compared to the wild type protein. By contrast, GFP-SUN1-S113D resembling the phosphorylated state allowed very efficient export of poly(A)+RNA. Furthermore, probing a possible role of the LINC complex component Nesprin-2 in this process we observed impaired mRNA export in Nesprin-2 knockdown cells. This effect might be independent of SUN1 as expression of a GFP tagged SUN-domain deficient SUN1, which no longer can interact with Nesprin-2, did not affect mRNA export.

  19. Application of artificial intelligence for nuclear power plant surveillance and diagnosis problems

    International Nuclear Information System (INIS)

    Monnier, B.; Ricard, B.; Doutre, J.L.; Martin-Mattei, C.; Fernandes, A.

    1991-01-01

    This paper presents three expert systems in the field of surveillance and diagnosis of nuclear power plants. Each application is described from the point of view of knowledge modeling. Then, a general knowledge model is proposed for a class of diagnosis problems. At the end, the paper shows the future frame of the surveillance of the nuclear power plant main components at EDF in which the greatest part of those expert systems will run

  20. Nuclear reactor pressure vessel surveillance capsule examinations. Application of American Society for Testing and Materials Standards

    International Nuclear Information System (INIS)

    Perrin, J.S.

    1978-01-01

    A series of pressure vessel surveillance capsules is installed in each commercial nuclear power plant in the United States. A capsule typically contains neutron dose meters, thermal monitors, tensile specimens, and Charpy V-notch impact specimens. In order to determine property changes of the pressure vessel resulting from irradiation, surveillance capsules are periodically removed during the life of a reactor and examined. There are numerous standards, regulations, and codes governing US pressure vessel surveillance capsule programmes. These are put out by the US Nuclear Regulatory Commission, the Boiler and Pressure Vessel Committee of the American Society of Mechanical Engineers, and the American Society for Testing and Materials (ASTM). A majority of the pertinent ASTM standards are under the jurisdiction of ASTM Committee E-10 on Nuclear Applications and Measurements of Radiation Effects. The standards, regulations, and codes pertaining to pressure vessel surveillance play an important role in ensuring reliability of the nuclear pressure vessels. ASTM E 185-73 is the Standard Recommended Practice for Surveillance Tests for Nuclear Reactors. This standard recommends procedures for both the irradiation and subsequent testing of surveillance capsules. ASTM E 185-73 references many additional specialized ASTM standards to be followed in specific areas of a surveillance capsule examination. A key element of surveillance capsule programmes is the Charpy V-notch impact test, used to define curves of fracture behaviour over a range of temperatures. The data from these tests are used to define the adjusted reference temperature used in determining pressure-temperature operating curves for a nuclear power plant. (author)

  1. ALS Associated Mutations in Matrin 3 Alter Protein-Protein Interactions and Impede mRNA Nuclear Export.

    Science.gov (United States)

    Boehringer, Ashley; Garcia-Mansfield, Krystine; Singh, Gurkaran; Bakkar, Nadine; Pirrotte, Patrick; Bowser, Robert

    2017-11-06

    Mutations in Matrin 3 have recently been linked to ALS, though the mechanism that induces disease in these patients is unknown. To define the protein interactome of wild-type and ALS-linked MATR3 mutations, we performed immunoprecipitation followed by mass spectrometry using NSC-34 cells expressing human wild-type or mutant Matrin 3. Gene ontology analysis identified a novel role for Matrin 3 in mRNA transport centered on proteins in the TRanscription and EXport (TREX) complex, known to function in mRNA biogenesis and nuclear export. ALS-linked mutations in Matrin 3 led to its re-distribution within the nucleus, decreased co-localization with endogenous Matrin 3 and increased co-localization with specific TREX components. Expression of disease-causing Matrin 3 mutations led to nuclear mRNA export defects of both global mRNA and more specifically the mRNA of TDP-43 and FUS. Our findings identify a potential pathogenic mechanism attributable to MATR3 mutations and further link cellular transport defects to ALS.

  2. An essential nuclear protein in trypanosomes is a component of mRNA transcription/export pathway.

    Directory of Open Access Journals (Sweden)

    Mariana Serpeloni

    Full Text Available In eukaryotic cells, different RNA species are exported from the nucleus via specialized pathways. The mRNA export machinery is highly integrated with mRNA processing, and includes a different set of nuclear transport adaptors as well as other mRNA binding proteins, RNA helicases, and NPC-associated proteins. The protozoan parasite Trypanosoma cruzi is the causative agent of Chagas disease, a widespread and neglected human disease which is endemic to Latin America. Gene expression in Trypanosoma has unique characteristics, such as constitutive polycistronic transcription of protein-encoding genes and mRNA processing by trans-splicing. In general, post-transcriptional events are the major points for regulation of gene expression in these parasites. However, the export pathway of mRNA from the nucleus is poorly understood. The present study investigated the function of TcSub2, which is a highly conserved protein ortholog to Sub2/ UAP56, a component of the Transcription/Export (TREX multiprotein complex connecting transcription with mRNA export in yeast/human. Similar to its orthologs, TcSub2 is a nuclear protein, localized in dispersed foci all over the nuclei -except the fibrillar center of nucleolus- and at the interface between dense and non-dense chromatin areas, proposing the association of TcSub2 with transcription/processing sites. These findings were analyzed further by BrUTP incorporation assays and confirmed that TcSub2 is physically associated with active RNA polymerase II (RNA pol II, but not RNA polymerase I (RNA pol I or Spliced Leader (SL transcription, demonstrating participation particularly in nuclear mRNA metabolism in T. cruzi. The double knockout of the TcSub2 gene is lethal in T. cruzi, suggesting it has an essential function. Alternatively, RNA interference assays were performed in Trypanosoma brucei. It allowed demonstrating that besides being an essential protein, its knockdown causes mRNA accumulation in the nucleus and

  3. Current nuclear industry practices with regard to the integration of surveillances

    International Nuclear Information System (INIS)

    Stewart, M.; Smith, C.

    1992-04-01

    Commercial nuclear industry practices regarding the integration of technical specification (TS) surveillance and maintenance activities are identified in this report. A questionnaire was developed and used to obtain current industry practices from NRC Regional personnel and INEL engineers with utility experience. Some of these practices indicate that the scheduling of TS surveillance and maintenance items could be more effectively coordinated. Also, must utilities do not formally consider risk implications when they are scheduling maintenance. Methodologies and approaches for proposing and evaluating changes to improve the integration of TS surveillance and maintenance activities have been identified for planned future work

  4. Implementation of nuclear material surveillance system based on the digital video capture card and counter

    International Nuclear Information System (INIS)

    Lee, Sang Yoon; Song, Dae Yong; Ko, Won Il; Ha, Jang Ho; Kim, Ho Dong

    2003-07-01

    In this paper, the implementation techniques of nuclear material surveillance system based on the digital video capture board and digital counter was described. The surveillance system that is to be developed is consist of CCD cameras, neutron monitors, and PC for data acquisition. To develop the system, the properties of the PCI based capture board and counter was investigated, and the characteristics of related SDK library was summarized. This report could be used for the developers who want to develop the surveillance system for various experimental environments based on the DVR and sensors using Borland C++ Builder

  5. A video imaging system and related control hardware for nuclear safeguards surveillance applications

    International Nuclear Information System (INIS)

    Whichello, J.V.

    1987-03-01

    A novel video surveillance system has been developed for safeguards applications in nuclear installations. The hardware was tested at a small experimental enrichment facility located at the Lucas Heights Research Laboratories. The system uses digital video techniques to store, encode and transmit still television pictures over the public telephone network to a receiver located in the Australian Safeguards Office at Kings Cross, Sydney. A decoded, reconstructed picture is then obtained using a second video frame store. A computer-controlled video cassette recorder is used automatically to archive the surveillance pictures. The design of the surveillance system is described with examples of its operation

  6. Radiation protection and environmental surveillance programme in and around Nuclear Fuel Cycle Facilities in India

    International Nuclear Information System (INIS)

    Tripathi, R.M.

    2018-01-01

    Radiation safety is an integral part of the operation of the Indian nuclear fuel cycle facilities and safety culture has been inculcated in all the spheres of its operation. Nuclear fuel cycle comprises of mineral exploration, mining, ore processing, fuel fabrication, power plants, reprocessing, waste management and accelerator facilities. Health Physics Division of BARC is entrusted with the responsibility of radiation protection and environmental surveillance in all the nuclear fuel cycle facilities

  7. Advances in noise analysis for nuclear plant surveillance and diagnostics

    International Nuclear Information System (INIS)

    Fry, N.; Clapp, N.E. Jr.; Sides, W.H. Jr.; Mihalczo, J.T.; King, W.T.

    1980-01-01

    An automated surveillance and baseline noise signature acquisition system is being demonstrated at Sequoyah-1. A nonperturbing method is also being developed for monitoring the subcritical reactivity during initial core loading in LWRs, in fuel storage and processing facilities, and during postaccident recovery operations such as Three Mile Island-2

  8. [Polyadenylated RNA and mRNA export factors in extrachromosomal nuclear domains of vitellogenic oocytes of the insect Tenebrio molitor].

    Science.gov (United States)

    Bogoliubov, D S; Kiselev, A M; Shabel'nikov, S V; Parfenov, V N

    2012-01-01

    The nucleus ofvitellogenic oocytes of the yellow mealworm, Tenebrio molitor, contains a karyosphere that consists of the condensed chromatin embedded in an extrachromosomal fibrogranular material. Numerous nuclear bodies located freely in the nucleoplasm are also observed. Amongst these bodies, counterparts of nuclear speckles (= interchromatin granule clusters, IGCs) can be identified by the presence of the marker protein SC35. Microinjections of fluorescently tagged methyloligoribonucleotide probes 2'-O-Me(U)22, complementary to poly(A) tails of RNAs, revealed poly(A)+ RNA in the vast majority of IGCs. We found that all T. molitor oocyte IGCs contain heterogeneous ribonucleoprotein (hnRNP) core protein Al that localizes to IGCs in an RNA-dependent manner. The extrachromosomal material of the karyosphere and a part of nucleoplasmic IGCs also contain the adapter protein Aly that is known to provide a link between pre-mRNA splicing and mRNA export. The essential mRNA export factor/receptor NXF1 was observed to colocalize with Aly. In nucleoplasmic IGCs, NXF1 was found to localize in an RNA-dependent manner whereas it is RNA-independently located in the extrachromosomal material of the karyosphere. We believe our data suggest on a role of the nucleoplasmic IGCs in mRNA biogenesis and retention in a road to nuclear export.

  9. Quality surveillance at Duke Power operating nuclear stations

    International Nuclear Information System (INIS)

    Bradley, W.H.

    1985-01-01

    The Duke Power quality assurance (QA) program originally had three levels of QA audits that reported results to different levels of management and in different time frames. It soon became obvious that although problems were being identified, considerable confusion was being created by reporting them to different levels. Additionally, it was recognized that all audits were dealing in history: events that had already occurred and therefore could not be averted. What was needed was a problem identification system focused on current activities. The Duke quality surveillance program was created by dropping the first level of audits and replacing it with an on-the-scene review system to identify current problems. The quality surveillance program is a success and will be even more so after surveillance personnel acquire additional technical expertise. To that end, a training program has been initiated that will stop just short of operator licensing in the operations area and will also include other areas such as health physics and chemistry

  10. RNA surveillance via nonsense-mediated mRNA decay is crucial for longevity in daf-2/insulin/IGF-1 mutant C. elegans.

    Science.gov (United States)

    Son, Heehwa G; Seo, Mihwa; Ham, Seokjin; Hwang, Wooseon; Lee, Dongyeop; An, Seon Woo A; Artan, Murat; Seo, Keunhee; Kaletsky, Rachel; Arey, Rachel N; Ryu, Youngjae; Ha, Chang Man; Kim, Yoon Ki; Murphy, Coleen T; Roh, Tae-Young; Nam, Hong Gil; Lee, Seung-Jae V

    2017-03-09

    Long-lived organisms often feature more stringent protein and DNA quality control. However, whether RNA quality control mechanisms, such as nonsense-mediated mRNA decay (NMD), which degrades both abnormal as well as some normal transcripts, have a role in organismal aging remains unexplored. Here we show that NMD mediates longevity in C. elegans strains with mutations in daf-2/insulin/insulin-like growth factor 1 receptor. We find that daf-2 mutants display enhanced NMD activity and reduced levels of potentially aberrant transcripts. NMD components, including smg-2/UPF1, are required to achieve the longevity of several long-lived mutants, including daf-2 mutant worms. NMD in the nervous system of the animals is particularly important for RNA quality control to promote longevity. Furthermore, we find that downregulation of yars-2/tyrosyl-tRNA synthetase, an NMD target transcript, by daf-2 mutations contributes to longevity. We propose that NMD-mediated RNA surveillance is a crucial quality control process that contributes to longevity conferred by daf-2 mutations.

  11. Surveillance of a nuclear reactor core by use of a pattern recognition method

    International Nuclear Information System (INIS)

    Invernizzi, Michel.

    1982-07-01

    A pattern recognition system is described for the surveillance of a PWR reactor. This report contains four chapters. The first one succinctly deals with statistical pattern recognition principles. In the second chapter we show how a surveillance problem may be treated by pattern recognition and we present methods for surveillances (detection of abnormalities), controls (kind of running recognition) and diagnotics (kind of abnormality recognition). The third chapter shows a surveillance method of a nuclear plant. The signals used are the neutron noise observations made by the ionization chambers inserted in the reactor. Abnormality is defined in opposition with the training set witch is supposed to be an exhaustive summary of normality. In the fourth chapter we propose a scheme for an adaptative recognition and a method based on classes modelisations by hyper-spheres. This method has been tested on simulated training sets in two-dimensional feature spaces. It gives solutions to problems of non-linear separability [fr

  12. Operating results obtained in a nuclear power plant with a sensor surveillance prototype

    International Nuclear Information System (INIS)

    Jacquot, J.P.; Poujol, A.; Beaubatie, J.; Ciaramitaro, W.

    1983-03-01

    Surveillance methods have been validated and specific equipment have been built to measure the response time of sensors from a nuclear power plant protection channel. The reason of the choice of this parameter is twofold: the sensor response time is representative of the sensor physical status and is also part of the overall channel response time. Two surveillance methods are used: noise analysis (by AR or PSD modeling), and loop current step response (for resistance thermometer detectors only). The methods were validated on test facilities and on nuclear power plants. Two test equipments were built and tested on plants. Results are represented and conclusions are drawn on the feasibility of such methods for sensor surveillance [fr

  13. Selective nuclear export of specific classes of mRNA from mammalian nuclei is promoted by GANP

    Science.gov (United States)

    Wickramasinghe, Vihandha O.; Andrews, Robert; Ellis, Peter; Langford, Cordelia; Gurdon, John B.; Stewart, Murray; Venkitaraman, Ashok R.; Laskey, Ronald A.

    2014-01-01

    The nuclear phase of the gene expression pathway culminates in the export of mature messenger RNAs (mRNAs) to the cytoplasm through nuclear pore complexes. GANP (germinal- centre associated nuclear protein) promotes the transfer of mRNAs bound to the transport factor NXF1 to nuclear pore complexes. Here, we demonstrate that GANP, subunit of the TRanscription-EXport-2 (TREX-2) mRNA export complex, promotes selective nuclear export of a specific subset of mRNAs whose transport depends on NXF1. Genome-wide gene expression profiling showed that half of the transcripts whose nuclear export was impaired following NXF1 depletion also showed reduced export when GANP was depleted. GANP-dependent transcripts were highly expressed, yet short-lived, and were highly enriched in those encoding central components of the gene expression machinery such as RNA synthesis and processing factors. After injection into Xenopus oocyte nuclei, representative GANP-dependent transcripts showed faster nuclear export kinetics than representative transcripts that were not influenced by GANP depletion. We propose that GANP promotes the nuclear export of specific classes of mRNAs that may facilitate rapid changes in gene expression. PMID:24510098

  14. Stochastic pattern recognition techniques and artificial intelligence for nuclear power plant surveillance and anomaly detection

    Energy Technology Data Exchange (ETDEWEB)

    Kemeny, L.G

    1998-12-31

    In this paper a theoretical and system conceptual model is outlined for the instrumentation, core assessment and surveillance and anomaly detection of a nuclear power plant. The system specified is based on the statistical on-line analysis of optimally placed instrumentation sensed fluctuating signals in terms of such variates as coherence, correlation function, zero-crossing and spectral density

  15. Stochastic pattern recognition techniques and artificial intelligence for nuclear power plant surveillance and anomaly detection

    International Nuclear Information System (INIS)

    Kemeny, L.G.

    1998-01-01

    In this paper a theoretical and system conceptual model is outlined for the instrumentation, core assessment and surveillance and anomaly detection of a nuclear power plant. The system specified is based on the statistical on-line analysis of optimally placed instrumentation sensed fluctuating signals in terms of such variates as coherence, correlation function, zero-crossing and spectral density

  16. Interactions between mRNA export commitment, 3'-end quality control, and nuclear degradation

    DEFF Research Database (Denmark)

    Libri, Domenico; Dower, Ken; Boulay, Jocelyne

    2002-01-01

    Several aspects of eukaryotic mRNA processing are linked to transcription. In Saccharomyces cerevisiae, overexpression of the mRNA export factor Sub2p suppresses the growth defect of hpr1 null cells, yet the protein Hpr1p and the associated THO protein complex are implicated in transcriptional el...... results show that several classes of defective RNPs are subject to a quality control step that impedes release from transcription site foci and suggest that suboptimal messenger ribonucleoprotein assembly leads to RNA degradation by Rrp6p....

  17. Maintenance quality surveillance for nuclear reactor instrument and control systems

    International Nuclear Information System (INIS)

    Clement, T.M.

    1976-01-01

    A description is given of a formal program of mandatory testing and inspection for assurance of reliability at the N-reactor. The techniques and procedures are called Equipment Maintenance Standards (EMS) and cover the nuclear plant systems which affect nuclear safety, environmental control, continuity of operation and reactor life. The scope of the program is such that all electrical, chemical, mechanical, and nuclear devices with interconnecting circuitry, linkages, and piping are functionally checked and/or inspected from sensors through actuating media necessary for carrying out the defined functions under startup, operating, and shutdown conditions and also under any credible accident condition. Records are kept of the AS FOUND results of the tests and inspections along with adjustments and repairs required to correct any out-of-limits conditions found. These records are accumulated and are used as a source for quantitative reliability information and for evaluating wear-out or design problems

  18. Standard protocol for conducting pre-operational environmental surveillance around nuclear facilities

    Energy Technology Data Exchange (ETDEWEB)

    Hegde, A G; Verma, P C; Rajan, M P [Health Safety and Environment Group, Bhabha Atomic Research Centre, Mumbai (India)

    2009-02-15

    This document presents the standard procedures for evaluation of site specific environmental transfer factors around NPP sites. The scope of this document is to provide standard protocol to be followed for conducting pre-operational environmental surveillance around nuclear facilities. Such surveillances have been proposed to be carried out by university professionals under DAE-BRNS projects. This document contains a common methodology in terms of sampling, processing, measurements and analysis of elemental/radionuclides, while keeping the site specific requirements also in place. (author)

  19. Standard protocol for conducting pre-operational environmental surveillance around nuclear facilities

    International Nuclear Information System (INIS)

    Hegde, A.G.; Verma, P.C.; Rajan, M.P.

    2009-02-01

    This document presents the standard procedures for evaluation of site specific environmental transfer factors around NPP sites. The scope of this document is to provide standard protocol to be followed for conducting pre-operational environmental surveillance around nuclear facilities. Such surveillances have been proposed to be carried out by university professionals under DAE-BRNS projects. This document contains a common methodology in terms of sampling, processing, measurements and analysis of elemental/radionuclides, while keeping the site specific requirements also in place. (author)

  20. Standard Practice for Design of Surveillance Programs for Light-Water Moderated Nuclear Power Reactor Vessels

    CERN Document Server

    American Society for Testing and Materials. Philadelphia

    2010-01-01

    1.1 This practice covers procedures for designing a surveillance program for monitoring the radiation-induced changes in the mechanical properties of ferritic materials in light-water moderated nuclear power reactor vessels. This practice includes the minimum requirements for the design of a surveillance program, selection of vessel material to be included, and the initial schedule for evaluation of materials. 1.2 This practice was developed for all light-water moderated nuclear power reactor vessels for which the predicted maximum fast neutron fluence (E > 1 MeV) at the end of license (EOL) exceeds 1 × 1021 neutrons/m2 (1 × 1017 n/cm2) at the inside surface of the reactor vessel. 1.3 This practice applies only to the planning and design of surveillance programs for reactor vessels designed and built after the effective date of this practice. Previous versions of Practice E185 apply to earlier reactor vessels. 1.4 This practice does not provide specific procedures for monitoring the radiation induced cha...

  1. The role of governmental authorities in the surveillance activities for quality assurance of nuclear power plants

    International Nuclear Information System (INIS)

    Koutsky, J.; Hrdlicka, Z.; Beranek, J.; Becvar, J.

    1982-01-01

    The paper deals with the legal and organizational aspects as well as with the principles and procedures of the quality assurance (QA) system for particular equipment of nuclear power plants in Czechoslovakia, and the role of governmental authorities in surveillance activities. The basic governmental document is the Decree No.5 (1979) of the Czechoslovak Atomic Energy Commission (CzAEC) on the QA of particular equipment in nuclear power plants with regard to nuclear safety. It is divided into ten sections: opening provisions; classification of the particular equipment; QA programmes; procedures of final elaboration of QA programme documentation; QA principles; QA during design; QA during manufacture, procurement, installation and commissioning; in-service inspection; QA during repair and modifications; and concluding provisions. Governmental surveillance is based on Governmental Resolution No.195 (1977). According to this resolution, inspection must be carried out by an organization which is independent of the manufacturing and operating organizations. This inspection is performed by the Czechoslovak Atomic Energy Commission (for nuclear safety) and by the Czech Safety Work Office and the Slovak Safety Work Office (for technical safety), together with subordinated regional divisions. The activities of these authorities are discussed. (author)

  2. Surveillance tests for light-water cooled nuclear power reactor vessels in IMEF

    International Nuclear Information System (INIS)

    Choo, Yong-Sun; Ahn, Sang-Bok; Park, Dae-Gyu; Jung, Yang-Hong; Yoo, Byung-Ok; Oh, Wan-Ho; Baik, Seung-Je; Koo, Dae-Seo; Lee, Key-Soon

    1999-01-01

    The surveillance tests for light-water cooled nuclear power reactor vessels were established to monitor the radiation-induced changes in the mechanical properties of ferritic materials in the beltline according to US NRC 10 CFR 50 App. G, US NRC RG1.99-rev.2, ASTM E185-82 and E185-94 in Irradiated Materials Examination Facility(IMEF). The surveillance capsule was transported from NPPs pool sites to KAERI IMEF by using a shipping cask. The capsule was cut and dismantled by capsule cutting machine and milling machine in M2 hot cell. Charpy tests and tension tests were performed in M5a and M5b hot cells respectively. Especially the EPMA located at hot lab was used to analyze the Ni and Cu wt% composition of base metal and weld for predicting the adjusted reference temperature(ART). The established process and test results were summarized in this paper. (author)

  3. Standard Guide for Conducting Supplemental Surveillance Tests for Nuclear Power Reactor Vessels, E 706 (IH)

    CERN Document Server

    American Society for Testing and Materials. Philadelphia

    2010-01-01

    1.1 This guide discusses test procedures that can be used in conjunction with, but not as alternatives to, those required by Practices E185 and E2215 for the surveillance of nuclear reactor vessels. The supplemental mechanical property tests outlined permit the acquisition of additional information on radiation-induced changes in fracture toughness, notch ductility, and yield strength properties of the reactor vessel steels. 1.2 This guide provides recommendations for the preparation of test specimens for irradiation, and identifies special precautions and requirements for reactor surveillance operations and postirradiation test planning. Guidance on data reduction and computational procedures is also given. Reference is made to other ASTM test methods for the physical conduct of specimen tests and for raw data acquisition.

  4. A study on surveillance equipment at the exit/entry control point of nuclear facility

    International Nuclear Information System (INIS)

    Park, C. S.; Kim, D. Y.; Cha, H. L.; Kim, H. D.; Hong, J. S.

    1999-01-01

    Exit/Entry control is an essential measure at both entrances of the protected area and vital area of nuclear facility at which physical protection is required under the relevant laws and regulations. Especially, when there are heavy traffics of personnel and process equipment in those areas, automated surveillance devices have to be introduced to timely and efficiently screen out internal and external adversaries from achieving their goals of stealing of nuclear material and/or sabotage of the facility. The major portion of this study involves with integration and processing of signals from radiation detector, metal detector, and image monitor. This integrated device together with positive personal identification device which will be reinforced in near future would contribute to the establishment of total exit/entry control point of nuclear facility

  5. Integration of artificial intelligence systems for nuclear power plants surveillance and diagnostics

    International Nuclear Information System (INIS)

    Chetry, Moon K.

    2012-01-01

    The objective of this program is to design, construct operate, test, and evaluate a prototype integrated monitoring and diagnostic system for a nuclear power plant. It is anticipated that this technology will have wide application to other complex systems (e.g., fossil power plants, chemical processing plants, and possibly air traffic control systems). Over the past decade, the University of Tennessee (UT) and others have carried out many projects utilizing various methodologies of artificial intelligence (expert systems, neural networks, fuzzy systems and genetic algorithms) to enhance the performance (safety, efficiency, reliability, and availability) of nuclear power plants. Investigations and studies have included a) instrumentation surveillance and calibration validation, b) inferential sensing to calibration of feed water venture flow during fouling, c) thermodynamic performance modeling with iterative improvement of plant heat rate, d) diagnosis of nuclear power plant transients

  6. Neutron dosimetry in EDF experimental surveillance programme for VVER-440 nuclear power plants

    International Nuclear Information System (INIS)

    Brumovsky, M.; Erben, O.; Novosad, P.; Zerola, L.; Hogel, J.; Trollat, C.

    2001-01-01

    Fourteen chains containing experimental surveillance material specimens of the VVER 440/213 nuclear power reactor pressure vessels were irradiated in the surveillance channels of the Nuclear Power Plant Dukovany in the Czech Republic. The irradiation periods were one, two or three cycles. The chains contained different number and types of containers, the omitted ones were replaced by chain elements. All of the containers were instrumented with wire neutron fluence detectors, some of the containers in the chain had spectrometric sets of neutron fluence monitors. For the absolute fluence values evaluation it was taken into account time history of the reactor power and local changes of the neutron flux along the reactor core height, correction factors due to the orientation of monitors with respect to the reactor core centre. Unfolding programs SAND-II or BASA-CF were used. The relative axial fluence distribution was obtained from the O-wire measurements. Neutron fluence values above 0.5 MeV energy and above 1.0 MeV energy in the container axis on the axial positions of the sample centres and fluence values in the geometric centre of the samples was calculated making use the exponential attenuation model of the incident neutron beam. Received fast neutron fluence values can be used as reference values to all VVER-440 type 213 nuclear power plant reactors. (author)

  7. Safety assessment and surveillance of decommissioning operations at DOE's nuclear facilities

    International Nuclear Information System (INIS)

    Cowgill, M.G.; Prochnow, D.; Worthington, P.R.

    1995-01-01

    A description is provided of a systematic approach currently being developed and deployed at the Department of Energy to obtain assurance that post-operational activities at nuclear facilities will be conducted in a safe manner. Using this approach, personnel will have available a formalized set of safety principles and associated question sets to assist them in the conducting of safety assessments and surveillance. Information gathered through this means will also be analyzed to determine if there are any generic complex-wide strengths or deficiencies associated with decommissioning activities and to which attention should be drawn

  8. Structure of Lipid Nanoparticles Containing siRNA or mRNA by Dynamic Nuclear Polarization-Enhanced NMR Spectroscopy.

    Science.gov (United States)

    Viger-Gravel, Jasmine; Schantz, Anna; Pinon, Arthur C; Rossini, Aaron J; Schantz, Staffan; Emsley, Lyndon

    2018-02-22

    Here, we show how dynamic nuclear polarization (DNP) NMR spectroscopy experiments permit the atomic level structural characterization of loaded and empty lipid nanoparticles (LNPs). The LNPs used here were synthesized by the microfluidic mixing technique and are composed of ionizable cationic lipid (DLin-MC3-DMA), a phospholipid (distearoylphosphatidylcholine, DSPC), cholesterol, and poly(ethylene glycol) (PEG) (dimyristoyl phosphatidyl ethanolamine (DMPE)-PEG 2000), as well as encapsulated cargoes that are either phosphorothioated siRNA (50 or 100%) or mRNA. We show that LNPs form physically stable complexes with bioactive drug siRNA for a period of 94 days. Relayed DNP experiments are performed to study 1 H- 1 H spin diffusion and to determine the spatial location of the various components of the LNP by studying the average enhancement factors as a function of polarization time. We observe a striking feature of LNPs in the presence and in the absence of encapsulating siRNA or mRNA by comparing our experimental results to numerical spin-diffusion modeling. We observe that LNPs form a layered structure, and we detect that DSPC and DMPE-PEG 2000 lipids form a surface rich layer in the presence (or absence) of the cargoes and that the cholesterol and ionizable cationic lipid are embedded in the core. Furthermore, relayed DNP 31 P solid-state NMR experiments allow the location of the cargo encapsulated in the LNPs to be determined. On the basis of the results, we propose a new structural model for the LNPs that features a homogeneous core with a tendency for layering of DSPC and DMPE-PEG at the surface.

  9. Experience and development of on-line BWR surveillance system at Onagawa nuclear power station unit-1

    International Nuclear Information System (INIS)

    Kishi, A.; Chiba, K.; Kato, K.; Ebata, S.; Ando, Y.; Sakamoto, H.

    1986-01-01

    ONAGAWA nuclear power station Unit-1 (Tohoku Electric Power Co.) is a BWR-4 nuclear power station of 524 MW electric power which started commercial operation in June 1984. To attain high reliability and applicability for ONAGAWA-1, Tohoku Electric Power Co. and Toshiba started a Research and Development project on plant surveillance and diagnosis from April 1982. Main purposes of this project are to: (1) Develop an on-line surveillance system and acquire its operating experience at a commercial BWR, (2) Assist in plant operation and maintenance by data acquisition and analysis, (3) Develop a new technique for plant surveillance and diagnosis. An outline of the project, operating experience gained from the on-line surveillance system and an introduction to new diagnosis techniques are reported in this paper. (author)

  10. Demonstration of an automated on-line surveillance system at a commercial nuclear power plant

    International Nuclear Information System (INIS)

    Smith, C.M.; Sweeney, F.J.

    1983-01-01

    As a first step in demonstrating the practicality of performing continuous on-line surveillance of the performance of nuclear steam supply systems using noise related techniques, Oak Ridge National Laboratory is operating a computerized noise signal data acquisition and processing system at the Sequoyah Unit 1 Nuclear Plant, an 1148 MWe four-loop Westinghouse pressurized water reactor (PWR) located near Chattanooga, Tennessee. The principal objective is to establish, with a degree of continuity and completeness not previously achieved, the long-term characteristics of signals from neutron detectors and process sensors in order to evaluate the feasibility of detecting and diagnosing anomalous reactor conditions by means of changes in these signals. The system is designed to automatically screen the gathered data, using a number of descriptors derived from the power spectra of the monitored signals, and thereby select for the noise analyst's perusal only those data which differ statistically from norms which the system has previously established

  11. Artificial intelligence applications to the surveillance and diagnostics of nuclear power plants

    International Nuclear Information System (INIS)

    Zwingelstein, G.; Monnier, B.

    1985-01-01

    Artificial intelligence has come of age; it can be applied with benefits in the nuclear industry for various applications to improve both plant safety and availability. This paper presents four rule-based expert systems currently under development at Electricite de France for the surveillance and the diagnostics of pressurized water reactors (PWRs). The objectives of these experiences with expert system projects are to demonstrate their feasibility, to provide an evaluation of their potential benefits through on-site implementation, to identify the problems due to the knowledge extraction and representation, and finally to specify the classes of problems where expert systems are needed and useful. The specific goals of these expert systems are to provide an aid for the detection and location of failures occurring in nuclear plants

  12. Statistical analysis on the fluence factor of surveillance test data of Korean nuclear power plants

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Gyeong Geun; Kim, Min Chul; Yoon, Ji Hyun; Lee, Bong Sang; Lim, Sang Yeob; Kwon, Jun Hyun [Nuclear Materials Safety Research Division, Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2017-06-15

    The transition temperature shift (TTS) of the reactor pressure vessel materials is an important factor that determines the lifetime of a nuclear power plant. The prediction of the TTS at the end of a plant’s lifespan is calculated based on the equation of Regulatory Guide 1.99 revision 2 (RG1.99/2) from the US. The fluence factor in the equation was expressed as a power function, and the exponent value was determined by the early surveillance data in the US. Recently, an advanced approach to estimate the TTS was proposed in various countries for nuclear power plants, and Korea is considering the development of a new TTS model. In this study, the TTS trend of the Korean surveillance test results was analyzed using a nonlinear regression model and a mixed-effect model based on the power function. The nonlinear regression model yielded a similar exponent as the power function in the fluence compared with RG1.99/2. The mixed-effect model had a higher value of the exponent and showed superior goodness of fit compared with the nonlinear regression model. Compared with RG1.99/2 and RG1.99/3, the mixed-effect model provided a more accurate prediction of the TTS.

  13. Integration of artificial intelligence systems for nuclear power plant surveillance and diagnostics

    International Nuclear Information System (INIS)

    Uhrig, R.E.; Hines, J.W.; Nelson, W.R.

    1998-01-01

    The objective of this program is to design, construct, operate, test, and evaluate a prototype integrated monitoring and diagnostic system for a nuclear power plant. It is anticipated that this technology will have wide application to other complex systems (e.g., fossil power plants, chemical processing plants, and possibly air traffic control systems). Over the past decade, the University of Tennessee (UT) and others have carried out many projects utilizing various methodologies of artificial intelligence (expert systems, neural networks, fuzzy systems, and genetic algorithms) to enhance the performance (safety, efficiency, reliability, and availability) of nuclear power plants. Investigations and studies have included a) instrumentation surveillance and calibration validation, b) inferential sensing to calibration of feedwater venturi flow during fouling, c) thermodynamic performance modeling with iterative improvement of plant beat rate, d) diagnosis of nuclear power plant transients, and e) increase in thermal power through monitoring of DNBR (Departure from Nucleate Boiling Regime). To increase the likelihood of these individual systems being used in a nuclear power plant, they must be integrated into a single system that operates virtually autonomously, collecting, interpreting, and providing information to the operators in a simple and understandable format. (author)

  14. Integration of artificial intelligence systems for nuclear power plant surveillance and diagnostics

    Energy Technology Data Exchange (ETDEWEB)

    Uhrig, R.E.; Hines, J.W.; Nelson, W.R.

    1998-07-01

    The objective of this program is to design, construct, operate, test, and evaluate a prototype integrated monitoring and diagnostic system for a nuclear power plant. It is anticipated that this technology will have wide application to other complex systems (e.g., fossil power plants, chemical processing plants, and possibly air traffic control systems). Over the past decade, the University of Tennessee (UT) and others have carried out many projects utilizing various methodologies of artificial intelligence (expert systems, neural networks, fuzzy systems, and genetic algorithms) to enhance the performance (safety, efficiency, reliability, and availability) of nuclear power plants. Investigations and studies have included a) instrumentation surveillance and calibration validation, b) inferential sensing to calibration of feedwater venturi flow during fouling, c) thermodynamic performance modeling with iterative improvement of plant beat rate, d) diagnosis of nuclear power plant transients, and e) increase in thermal power through monitoring of DNBR (Departure from Nucleate Boiling Regime). To increase the likelihood of these individual systems being used in a nuclear power plant, they must be integrated into a single system that operates virtually autonomously, collecting, interpreting, and providing information to the operators in a simple and understandable format. (author)

  15. Experience gained with the development of a performance test program for the monitoring and surveillance systems in the Embalse nuclear power plant

    International Nuclear Information System (INIS)

    Arestin, M.L.

    1988-01-01

    The monitoring and surveillance system for Embalse nuclear power plant are presented. The reactor, the nuclear fuel management, the equipments from monitoring and surveillance system, the activities developed by the AIEA experts in each mission of this test program and the management of the components for this test program in Argentine are described. (E.G.) [pt

  16. Nuclear Export of Pre-Ribosomal Subunits Requires Dbp5, but Not as an RNA-Helicase as for mRNA Export.

    Science.gov (United States)

    Neumann, Bettina; Wu, Haijia; Hackmann, Alexandra; Krebber, Heike

    2016-01-01

    The DEAD-box RNA-helicase Dbp5/Rat8 is known for its function in nuclear mRNA export, where it displaces the export receptor Mex67 from the mRNA at the cytoplasmic side of the nuclear pore complex (NPC). Here we show that Dbp5 is also required for the nuclear export of both pre-ribosomal subunits. Yeast temperature-sensitive dbp5 mutants accumulate both ribosomal particles in their nuclei. Furthermore, Dbp5 genetically and physically interacts with known ribosomal transport factors such as Nmd3. Similar to mRNA export we show that also for ribosomal transport Dbp5 is required at the cytoplasmic side of the NPC. However, unlike its role in mRNA export, Dbp5 does not seem to undergo its ATPase cycle for this function, as ATPase-deficient dbp5 mutants that selectively inhibit mRNA export do not affect ribosomal transport. Furthermore, mutants of GLE1, the ATPase stimulating factor of Dbp5, show no major ribosomal export defects. Consequently, while Dbp5 uses its ATPase cycle to displace the export receptor Mex67 from the translocated mRNAs, Mex67 remains bound to ribosomal subunits upon transit to the cytoplasm, where it is detectable on translating ribosomes. Therefore, we propose a model, in which Dbp5 supports ribosomal transport by capturing ribosomal subunits upon their cytoplasmic appearance at the NPC, possibly by binding export factors such as Mex67. Thus, our findings reveal that although different ribonucleoparticles, mRNAs and pre-ribosomal subunits, use shared export factors, they utilize different transport mechanisms.

  17. Radiation hygienic annual report 2012. General environmental radioactivity and radiation surveillance in the vicinity of nuclear facilities in Bavaria

    International Nuclear Information System (INIS)

    Pfau, T.; Bernkopf, J.; Klement, R.; Bayerisches Landesamt fuer Umwelt, Augsburg

    2013-01-01

    The radiation hygienic annual report 2012 includes the following issues: (1) Introduction: Legal aspects of the surveillance, implementation of the radiation protection law, nuclear facility sites in Bavaria, interim storage facilities in Bavaria. (2) Natural radioactivity surveillance: measured data for the exposure paths air, water, food chain land, food chain water, residuals and waste. (3) Radiation surveillance in the vicinity of nuclear facilities in Bavaria: measures for air, precipitation, soils, plants, food chain land, milk and milk products, surface water, food chain water, drinking and ground water; measured data in the vicinity of NNP Isar 1 bd Isar 2 (KKI1/KKI2), NPP Gundremmingen (KGG), NPP Grafenrheinfeld (KKG), research neutron source Muenchen FRM II; emissions, meteorological conditions, spreading calculations.

  18. A reliability program for emergency diesel generators at nuclear power plants: Maintenance, surveillance, and condition monitoring

    International Nuclear Information System (INIS)

    Lofgren, E.V.; Henderson, W.; Burghardt, D.; Kripps, L.; Rothleder, B.

    1988-12-01

    This report is a companion report on NUREG/CR-5078, Volume 1, ''A Reliability Program for Emergency Diesel Generators at Nuclear Power Plants: Program Structure.'' The purpose of this report is to provide technical findings and insights related to: failure evaluation, troubleshooting, maintenance, surveillance, and condition monitoring. Examples and recommendations are provided for each of these areas based on actual emergency diesel generator (EDG) operating experience and the opinions of diesel generator experts. This report expands the more general guidance provided in Volume 1. In addition, a discussion of EDG interactions with other plant systems (e.g., instrument, air, service water, dc power) is provided since experience has shown that these support systems and their operation can adversely affect EDG reliability. Portions of this report have been designed for use by onsite personnel for evaluating operational characteristics of EDGs. 5 refs., 8 figs., 7 tabs

  19. Validity and design of environmental surveillance systems for operating nuclear power plants. Final report

    International Nuclear Information System (INIS)

    Eichholz, G.G.

    1977-12-01

    The composition and procedures of environmental surveillance programs at completed and operating nuclear power plants have been examined with respect to their validity, continuing significance and cost. It was found that many programs contain components that are mainly an extension of preoperational baseline measurements that need not be continued indefinitely and that others lack the statistical validity to make their continued application meaningful. To identify the practical limits imposed by counting statistics and realistic equipment capacity measurements were done on iodine-131 and cesium-137 containing samples to establish detectability limits and proportionate costs for sample preparation and counting. It was found that under commercial conditions effective detectability limits and expected confidence limits were substantially higher than those mentioned in NRC Regulatory Guides. This imposes a need for either selecting fewer samples and counting them for longer times or accepting a lesser accuracy on more samples, within the bounds of reasonable cost per sample

  20. Experience in surveillance of the prestress of concrete reactor vessels in Wylfa nuclear power station

    International Nuclear Information System (INIS)

    Dawson, P.; Paton, A.A.; Walsh, S.R.

    1989-01-01

    This paper describes experience gained in the in-service surveillance of the prestressing system for the prestressed concrete reactor vessels (PCRVs) at Wylfa nuclear power station. The paper gives details of results for the prestressing system obtained from the statutory in-service inspection program of the PCRVs. The program includes a detailed examination of a selection of prestressing tendon anchorages, anchorage load checks using a lift-off technique on a one percent sample of tendons and corrosion inspection of samples of prestressing strand and determination of their mechanical properties. The results obtained from the above in-service inspections have shown that the prestressing system continues to function within its design limits

  1. Medical surveillance of nuclear power plant workers during reactor shutdown using whole-body counting and excretion analysis

    International Nuclear Information System (INIS)

    Le Roux-Desmis, C.

    1987-01-01

    After a review of radioactivity basis and radiation protection principles, the various aspects of medical surveillance of nuclear power plant workers during reactor shutdown, are presented. Internal contamination incidents that happened during 1986-1987 shutdown of Paluel reactor are exposed. Internal contamination levels are evaluated using whole-body counting and radionuclide determination in feces and urine and compared with dose limits [fr

  2. Nuclear disarmament. Options for the coming non-proliferation treaty surveillance cycle; Nukleare Abruestung. Optionen fuer den kommenden Ueberpruefungszyklus des NVV

    Energy Technology Data Exchange (ETDEWEB)

    Mueller, Harald

    2011-07-01

    The report is aimed on the nuclear disarmament discussion with respect to the disagreement of nuclear weapon states and those without nuclear weapons, esp. the non-aligned movement (NAM) concerning the non-proliferation treaty. The report covers the following issues: The role of the non-proliferation treaty, nuclear disarmament in the last surveillance conference 2010, the different disarmament philosophies, the possibilities of bridging the disagreement, further disarmament options for the future non-proliferation treaty surveillance cycle, German options for the future surveillance cycle.

  3. Environmental surveillance status and assessment of the marking of ground waters and waterways around nuclear sites and radioactive waste old warehousing sites. Report for the High Committee for transparency and information on nuclear safety

    International Nuclear Information System (INIS)

    2008-01-01

    After a recall of the general objectives of environment radioactivity surveillance (notions about origin and behaviour of radioactive species in the environment, role of surveillance), this report describes the environment surveillance organisation and the investigations performed by the IRSN. It presents an overview of the current knowledge of the radiological status around the various French nuclear sites (radioactive effluent rejections, nuclear waste old warehousing sites, surface waters and soils, ground waters). It recalls and describes how surveillance results are published by the different involved parties. Each site is more precisely presented in appendix with its geological context, its surveillance equipment and results

  4. Surveillance of PLUS7TM fuel for PWR nuclear power plant

    International Nuclear Information System (INIS)

    Jang, Y. K.; Kim, J. I.; Shin, J. C.; Chung, J. G.; Chung, S. K.; Kim, M. S.; Lee, T. H.; Yoon, Y. B.; Kim, T. W.

    2012-01-01

    The surveillance program on the advanced nuclear fuel of PLUS TM developed for Optimized Power Reactors of 1000 MWe (OPR1000s) and Advanced Power Reactors of 1400 MWe (APR1400s) in Korea was completed in the early of 2011. This fuel had been jointly developed through the extensive out-of-pile tests with Westinghouse for three years since 1999. The irradiation tests for the in-reactor verification using four lead test assemblies (LTAs) had been started in Ulchin unit 3 in 2002. During the overhaul period after each irradiation test, the eight (8) burnup-dependent parameters were measured without disassembling using the precise measurement systems in pool-side. After three cycle irradiations, one test assembly was disassembled and the rod-wise inspection on twenty rods was performed. During this stage, five (5) parameters were measured and evaluated. Among these twenty rods, ten rods including skeleton were sent to hot-cell test facility for further detailed examination and are currently being examined. After in-reactor verifications during two cycles, this fuel was commercially supplied to eight (8) OPR1000s sequentially. Currently all eight (8) OPR1000s were replaced with this fuel. In addition, this fuel is going to be supplied to four (4) APR1400s being constructed in Braka, UAE as well as four(4) OPR1000s and four(4) APR1400s being constructed in Korea. In the meanwhile, the surveillance program for the commercially supplied fuel has been launched to confirm growth, creep, corrosion and deformation, etc. obtained during LTA irradiation. Four (4) limiting fuel assemblies, that is, two (2) assemblies to be discharged after 2 cycle irradiations and the other two (2) after 3 cycle irradiations were selected for this surveillance program. Irradiation data of commercially supplied fuels are compared and confirmed to LTA irradiation performance behaviors on this paper. Among the eight (8) burnup-dependent parameters, the interesting ones were irradiation

  5. Maintenance, surveillance and in-service inspection in nuclear power plants. Safety guide

    International Nuclear Information System (INIS)

    2002-01-01

    Effective maintenance, surveillance and in-service inspection (MS and I) are essential for the safe operation of a nuclear power plant. The objective of this Safety Guide is to provide recommendations and guidance for MS and I activities to ensure that SSCs important to safety are available to perform their functions in accordance with the assumptions and intent of the design. This Safety Guide covers the organizational and procedural aspects of MS and I. However, it does not give detailed technical advice in relation to particular items of plant equipment, nor does it cover inspections made for and/or by the regulatory body. This Safety Guide provides recommendations and guidance for preventive and remedial measures, including testing, surveillance and in-service inspection, that are necessary to ensure that all plant structures, systems and components (SSCs) important to safety are capable of performing as intended. This Safety Guide covers measures for fulfilling the organizational and administrative requirements for: establishing and implementing schedules for preventive and predictive maintenance, repairing defective plant items, selecting and training personnel, providing related facilities and equipment, procuring stores and spare parts, and generating, collecting and retaining maintenance records for establishing and implementing an adequate feedback system for information on maintenance. MS and I should be subject to quality assurance in relation to all aspects important to safety. Quality assurance has been dealt with in detail in other IAEA safety standards and is covered here only in specific instances, for emphasis. In Section 2, a concept of MS and I is presented and the interrelationship between maintenance, surveillance and inspection is discussed. Section 3 concerns the functions and responsibilities of different organizations involved in MS and I activities. Section 4 provides recommendations and guidance on such organizational aspects as

  6. Maintenance, surveillance and in-service inspection in nuclear power plants. Safety guide

    International Nuclear Information System (INIS)

    2005-01-01

    Effective maintenance, surveillance and in-service inspection (MS and I) are essential for the safe operation of a nuclear power plant. The objective of this Safety Guide is to provide recommendations and guidance for MS and I activities to ensure that SSCs important to safety are available to perform their functions in accordance with the assumptions and intent of the design. This Safety Guide covers the organizational and procedural aspects of MS and I. However, it does not give detailed technical advice in relation to particular items of plant equipment, nor does it cover inspections made for and/or by the regulatory body. This Safety Guide provides recommendations and guidance for preventive and remedial measures, including testing, surveillance and in-service inspection, that are necessary to ensure that all plant structures, systems and components (SSCs) important to safety are capable of performing as intended. This Safety Guide covers measures for fulfilling the organizational and administrative requirements for: establishing and implementing schedules for preventive and predictive maintenance, repairing defective plant items, selecting and training personnel, providing related facilities and equipment, procuring stores and spare parts, and generating, collecting and retaining maintenance records for establishing and implementing an adequate feedback system for information on maintenance. MS and I should be subject to quality assurance in relation to all aspects important to safety. Quality assurance has been dealt with in detail in other IAEA safety standards and is covered here only in specific instances, for emphasis. In Section 2, a concept of MS and I is presented and the interrelationship between maintenance, surveillance and inspection is discussed. Section 3 concerns the functions and responsibilities of different organizations involved in MS and I activities. Section 4 provides recommendations and guidance on such organizational aspects as

  7. Ddx19 links mRNA nuclear export with progression of transcription and replication and suppresses genomic instability upon DNA damage in proliferating cells.

    Science.gov (United States)

    Hodroj, Dana; Serhal, Kamar; Maiorano, Domenico

    2017-09-03

    The DEAD-box Helicase 19 (Ddx19) gene codes for an RNA helicase involved in both mRNA (mRNA) export from the nucleus into the cytoplasm and in mRNA translation. In unperturbed cells, Ddx19 localizes in the cytoplasm and at the cytoplasmic face of the nuclear pore. Here we review recent findings related to an additional Ddx19 function in the nucleus in resolving RNA:DNA hybrids (R-loops) generated during collision between transcription and replication, and upon DNA damage. Activation of a DNA damage response pathway dependent upon the ATR kinase, a major regulator of replication fork progression, stimulates translocation of the Ddx19 protein from the cytoplasm into the nucleus. Only nuclear Ddx19 is competent to resolve R-loops, and down regulation of Ddx19 expression induces DNA double strand breaks only in proliferating cells. Overall these observations put forward Ddx19 as an important novel mediator of the crosstalk between transcription and replication.

  8. Skeletal changes in osteoprotegerin and receptor activator of nuclear factor-κb ligand mRNA levels in primary hyperparathyroidism

    DEFF Research Database (Denmark)

    Stilgren, L.S.; Rettmer, E.; Eriksen, E. F.

    2004-01-01

    , and treatment of PHPT were included. A transiliac bone biopsy was done before (n = 24) and 12 months after parathyroidectomy (PTX) (n = 21). Biopsies were frozen in liquid nitrogen and RNA extracted using Trizol. A competitive RT-PCR assay for RANKL and OPG mRNA using artificial cDNA standards was developed...

  9. Advanced Surveillance, Diagnostic and Prognostic Techniques in Monitoring Structures, Systems and Components in Nuclear Power Plants

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2013-09-15

    direction of research, development and demonstration in this area. The technologies discussed in this project are intended to establish the state of the art in surveillance, diagnostics and prognostics (SDP) technologies for equipment and process health monitoring in nuclear facilities. It is also intended to identify technology gaps and research needs of the nuclear industry in the area of SDP. The report draws on the conventional SDP technologies, as well as the latest tools, algorithms and techniques that have emerged over the last few years, especially in enabling technologies including fast data acquisition, data storage, data qualification and data analysis algorithms, such as empirical and physical modelling techniques. These new tools have made it possible to identify problems earlier and with better resolution. The significance of the material presented in this report is that it contributes not only to the current needs of the nuclear industry but also to the design improvements of the next generation of reactors. For example, the nuclear industry is currently striving to operate the plants for up to 80 years or more, as the value of nuclear assets has risen in recent years, resulting partly from environmental concerns with fossil energy production, as well as increased future demand for base load electricity. This long term operation (LTO) or life extension goal of the nuclear industry has stimulated renewed interest in more frequent monitoring of equipment to guard against ageing effects, not to mention the economic benefits that SDP implementation can produce, and contributions to radiation exposure that is as low as reasonably achievable, reduction of human errors, and optimized maintenance. Together with capabilities that enhance situational awareness, the technologies described in this report will enable more holistic management of plant structures, systems and components (SSCs), maintain high capacity factor in LTO and enable higher levels of safe operation

  10. Advanced Surveillance, Diagnostic and Prognostic Techniques in Monitoring Structures, Systems and Components in Nuclear Power Plants

    International Nuclear Information System (INIS)

    2013-01-01

    direction of research, development and demonstration in this area. The technologies discussed in this project are intended to establish the state of the art in surveillance, diagnostics and prognostics (SDP) technologies for equipment and process health monitoring in nuclear facilities. It is also intended to identify technology gaps and research needs of the nuclear industry in the area of SDP. The report draws on the conventional SDP technologies, as well as the latest tools, algorithms and techniques that have emerged over the last few years, especially in enabling technologies including fast data acquisition, data storage, data qualification and data analysis algorithms, such as empirical and physical modelling techniques. These new tools have made it possible to identify problems earlier and with better resolution. The significance of the material presented in this report is that it contributes not only to the current needs of the nuclear industry but also to the design improvements of the next generation of reactors. For example, the nuclear industry is currently striving to operate the plants for up to 80 years or more, as the value of nuclear assets has risen in recent years, resulting partly from environmental concerns with fossil energy production, as well as increased future demand for base load electricity. This long term operation (LTO) or life extension goal of the nuclear industry has stimulated renewed interest in more frequent monitoring of equipment to guard against ageing effects, not to mention the economic benefits that SDP implementation can produce, and contributions to radiation exposure that is as low as reasonably achievable, reduction of human errors, and optimized maintenance. Together with capabilities that enhance situational awareness, the technologies described in this report will enable more holistic management of plant structures, systems and components (SSCs), maintain high capacity factor in LTO and enable higher levels of safe operation

  11. An overview of background environmental radiological surveillance around Kudankulam nuclear power project site

    International Nuclear Information System (INIS)

    Rajan, M.P.; Vijayakumar, B.; George, Thomas; Rajan, P.S.; Rana, B.K.; Kumar, M.; Hegde, A.G.; Chougaonkar, M.P.

    2005-01-01

    A modern Environmental Survey Laboratory (ESL) equipped with state-of-art nuclear counting, chemical and ecological equipment's has been set up at Kudankulam at the very beginning of construction activities to carry out pre operational and baseline radioactivity measurements. Construction activities for the two VVER type 1000 MWe reactors at the site are progressing ahead of schedule and the first unit is slated to be critical in the year 2007. This paper presents the results of environmental radiological surveillance carried out for the last one year in Kudankulam environs. General radiation background in the area varies in the range of 0.1 - 0.7 μGy/h and elevated levels as high as 20 μGy/h are noticed in beach areas. The major contribution to the background radiation in high background areas comes from 232 Th and its daughter products. The concentrations of 40 K are of normal order and comparable to other NPP sites and normal background areas. The concentrations of fallout nuclides are very low and are at global fallout levels. (author)

  12. Sensor signal analysis by neural networks for surveillance in nuclear reactors

    International Nuclear Information System (INIS)

    Keyvan, S.; Rabelo, L.C.

    1992-01-01

    The application of neural networks as a tool for reactor diagnostics is examined here. Reactor pump signals utilized in a wear-out monitoring system developed for early detection of the degradation of a pump shaft are analyzed as a semi-benchmark test to study the feasibility of neural networks for monitoring and surveillance in nuclear reactors. The Adaptive Resonance Theory (ART 2 and ART 2-A) paradigm of neural networks is applied in this study. The signals are collected signals as well as generated signals simulating the wear progress. The wear-out monitoring system applies noise analysis techniques, and is capable of distinguishing these signals apart and providing a measure of the progress of the degradation. This paper presents the results of the analysis of these data, and provides an evaluation on the performance of ART 2-A and ART 2 for reactor signal analysis. The selection of ART 2 is due to its desired design principles such as unsupervised learning, stability-plasticity, search-direct access, and the match-reset tradeoffs

  13. On line surveillance of large systems: applications to nuclear and chemical plant

    International Nuclear Information System (INIS)

    Zwingelstein, G.

    1978-01-01

    An on line surveillance method for large scale and distributed parameter systems is achieved by comparing in real time the internal physical parameter values to the reference values. It is shown that the following steps are necessary: modeling, model validation using dynamic testing and on line estimation of parameters. For large scale systems where only few outputs are measurable, an estimation algorithm was developed, selecting the measurable output giving the minimum variance of the physical parameters. This estimation scheme uses a quasilinearization technique associated to the sensitivity equation and the recursive least squares techniques. For large scale systems of order greater than 100, two versions of the estimation scheme are proposed to decrease the computation time. An application to a nuclear reactor core (state variable model of order 29) is proposed and used real data. For distributed systems the estimation scheme was developed with either measurements at fixed time or at fixed space. The estimation algorithm selects the set of measurements that gives the minimum variance of the estimates. An application to a liquid-liquid extraction column, modelized by a set of four coupled partial differential equations, demonstrates the efficiency of the method

  14. New nuclear scanning and surveillance systems for global security and safeguards

    International Nuclear Information System (INIS)

    Kemeny, L. G.

    2003-01-01

    This paper discusses new innovative techniques for both cargo and personnel scanning and plant and infrastructure surveillance and protection. It contains Intellectual Property and some of the systems described are covered by Patents. For example, a typical container inspection system is based on Pulsed Fast Neutron Analysis operating on the following principles: 1. An accelerator produced pulses of fast neutrons, which interact with the elemental composition of the cargo under inspection. In a manner similar to radar scanning the timing and positioning of the pulsed neutrons indicates where the interactions occurs. These interactions initiate the emission of gamma radiation which characterises the elemental composition and which is collected by sensor arrays. 2. The gamma ray signals are analysed in a high speed processor which identifies the presence and location of the chemical element combinations in all types of contraband. These may be drugs, explosives or nuclear material. 3. High resolution images display the location and shape of all contraband in the cargo under inspection. An x-ray like image of the cargo can also be provided. Because the scanning system software already contains standard gamma ray material signatures, the need for time consuming and unreliable manual interpretation of complicated images obtained in x-ray scanning systems is completely eliminated

  15. SMART instruments for radiological surveillance at the back end of nuclear fuel cycle

    International Nuclear Information System (INIS)

    Pendharkar, K.A.; Jauhri, G.S.; Ganesh, G.; Kulkarni, V.V.

    2001-01-01

    The back end of Nuclear Fuel Cycle mainly consists of Fuel Reprocessing Plant and Waste Management Plant for treatment of different types of wastes generated during processing of spent fuel. A fuel reprocessing plant handles annually several million curies of fission product activity and few hundred kg of plutonium. A Waste Management Facility associated with a reprocessing plant also handles several million curies of fission product activity. In both the plants several types of radiological measurements have to be carried out to ensure that the individual doses are well below regulatory limits and release of radioactivity to environment (through stack and through liquid effluent) is below the limit stipulated in technical specifications of the plant. The measurements comprise individual external dose, measurement of radiation level in different areas of the plant, assessment of air-borne activity due to plutonium and fission products in different areas of the plant, radioactivity release to environment through liquid effluents and through stack. In order to carry out the above mentioned measurements large number of different types of instruments are required. The existing instruments are analog instruments. These instruments have served well. However they have certain limitations with respect to flexibility and extra functionality. In this respect the 'SMART' instruments have distinct advantages. The advantages, that are offered by the 'SMART' instrument in making the radiological surveillance programme more effective, are brought out in the paper. (author)

  16. Development of containers sealing system like part of surveillance program of the vessel in nuclear power plants

    International Nuclear Information System (INIS)

    Romero C, J.; Hernandez C, R.; Fernandez T, F.; Rocamontes A, M.; Perez R, N.

    2009-10-01

    The owners of nuclear power plants should be demonstrate that the embrittlement effects by neutronic radiation do not commit the structural integrity from the pressure vessel of nuclear reactors, during conditions of routine operation and below postulate accident. For this reason, there are surveillance programs of vessels of nuclear power plants, in which are present surveillance capsules. A surveillance capsule is compound by the support, six containers for test tubes and dosimeters. The containers for test tubes are of two types: rectangular container for test tubes, Charpy V and Cylindrical Container for tension test tubes. These test tubes are subject to a same or bigger neutronic flow to that of vessel, being representative of vessel mechanical conditions. The test tubes are rehearsed to watch over the increase of embrittlement that presents the vessel. This work describes the development of welding system to seal the containers for test tubes, these should be filled with helium of ultra high purity, to a pressure of an atmosphere. In this system the welding process Gas Tungsten Arc Welding is used, a hermetic camera that allows to place the containers with three grades of freedom, a vacuum subsystem and pressure, high technology equipment's like: power source with integrated computer, arc starter of high frequency, helium flow controller, among others. Finally, the advances in the inspection system for the qualification of sealing system are mentioned, system that should measure the internal pressure of containers and the helium purity inside these. (Author)

  17. Surveillance Report on SAVY-4000 and Hagan Nuclear Material Storage Containers for FY 2017

    Energy Technology Data Exchange (ETDEWEB)

    Reeves, Kirk Patrick [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Karns, Tristan [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Weis, Eric [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Oka, Jude M. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Smith, Paul Herrick [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Stone, Timothy Amos [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Narlesky, Joshua Edward [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2017-12-14

    In accordance with the SAVY-4000 Surveillance Plan [1] and DOE M441.1-1 requirements, storage container surveillance continued through fiscal year 2017 at Los Alamos National Laboratory. Surveillance items for the year consisted of 8 SAVY-4000 storage containers, 8 Hagan containers, and 39 SAVY-4000 transfer containers. The SAVY-4000 surveillance items ranged in age from 1 year to 5.6 years and the Hagan containers ranged in age from 6.3 years to 17.6 years. The surveillance containers for this year were selected primarily to better understand the extent of corrosion of the stainless steel components of the containers. Accelerated aging studies indicate that the O-ring and filter components of the SAVY-4000 will last at least 40 years under LANL storage conditions. However, the observation of corrosion on the inside of SAVY-4000 and Hagan surveillance containers has shifted the emphasis to understanding both the nature and the extent of corrosion on the stainless steel body. The restriction on handling soluble residues greater than 500 grams continued this year, delaying the surveillance of some items that was scheduled in earlier surveillance plans.

  18. Long Term Storage with Surveillance of Canadian Prototype Nuclear Power Reactors

    International Nuclear Information System (INIS)

    Janzen, Rick

    2008-01-01

    Atomic Energy of Canada (AECL) was originally formed by the government of Canada in 1952 to perform research in radiation and nuclear areas. In the mid 1950's Canada decided to limit itself to peaceful uses of nuclear energy and AECL embarked on several research and development programs, one of them being the development of nuclear power plants. This led to the development of the CANDU TM design of heavy water power reactors, of which there are now 29 operating around the world. This presentation discusses the present state of the first two CANDU TM prototype reactors and a prototype boiling light water reactor and lessons learnt after being in a long-term storage with surveillance state for more than 20 years. AECL facilities undergo decommissioning by either a prompt or a deferred removal approach. Both approaches are initiated after an operating facility has been declared redundant and gone through final operational shutdown. For the deferred approach, initial decommissioning activities are performed to put the facility into a sustainable, safe, shutdown state to minimize the hazards and costs of the ensuing extended storage with surveillance (SWS) or Safestor phase. At the appropriate time, the facility is dismantled and removed, or put into a suitable condition for re-use. AECL has a number of facilities that were built during its history, and some of these are now redundant or will become redundant in the near future. The deferred removal approach is part of AECL's decommissioning strategy for several reasons: 1. Reduction in radiation doses to workers during the final dismantling, 2. No facilities are available yet in Canada for the management of quantity of wastes arising from decommissioning, 3. Financial constraints presented by the number of facilities that will undergo decommissioning, compared to the availability of funds to carry out the work. This has led to the development of a comprehensive decommissioning plan that includes all of AECL's redundant

  19. Application of an automatic pattern recognition for aleatory signals for the surveillance of nuclear reactor and rotating machinery

    International Nuclear Information System (INIS)

    Nascimento, J.A. do.

    1982-02-01

    An automatic pattern recognition program PSDREC, developed for the surveillance of nuclear reactor and rotating machinery is described and the relevant theory is outlined. Pattern recognition analysis of noise signals is a powerful technique for assessing 'system normality' in dynamic systems. This program, with applies 8 statistical tests to calculated power spectral density (PSD) distribution, was earlier installed in a PDP-11/45 computer at IPEN. To analyse recorded signals from three systems, namely an operational BWR power reactor (neutron signals), a water pump and a diesel engine (vibration signals) this technique was used. Results of the tests are considered satisfactory. (Author) [pt

  20. Operational experiences on the Borssele nuclear power plant using computer based surveillance and diagnostic system on-line

    International Nuclear Information System (INIS)

    Turkcan, E.; Quaadvliet, W.H.J.; Peeters, T.T.J.M.; Verhoef, J.P

    1991-06-01

    The on-line monitoring and diagnostics system of Borssele nuclear power plant (NPP), designed and established by the ECN Energy Research Foundation, has been operating continuously since 1983. The system is extended in form of multiprocessing, multi-tasking structure performing real-time monitoring, on-line reactor parameters' calculation, data-base preparation for expert systems and providing early information on possible malfunctions even in the incipient stage making alert by passive alarms. The system realized has already been operating in the course of 7 fuel cycles of the reactor starting from start-up through normal power operation. An expert system operating on the VAX work station is added to the surveillance and diagnostics system for data base management of the observed physical parameters relevant to the NPP under supervision. The paper highlights the surveillance and diagnostic modules involved, in their actual hierarchical form in use, presents theoretical considerations applied to the design of the surveillance system together with the results obtained through the 12th to 17th fuel cycles of the NPP including start-ups and shut-downs and reveals the experience thus gained by both utility and ECN through the application of the system described. (author). 19 refs.; 4 figs

  1. Heterogeneous nuclear ribonuclear protein K interacts with Sindbis virus nonstructural proteins and viral subgenomic mRNA

    International Nuclear Information System (INIS)

    Burnham, Andrew J.; Gong, Lei; Hardy, Richard W.

    2007-01-01

    Alphaviruses are a group of arthropod-borne human and animal pathogens that can cause epidemics of significant public health and economic consequence. Alphavirus RNA synthesis requires four virally encoded nonstructural proteins and probably a number of cellular proteins. Using comparative two-dimensional electrophoresis we were able to identify proteins enriched in cytoplasmic membrane fractions containing viral RNA synthetic complexes following infection with Sindbis virus. Our studies demonstrated the following: (i) the host protein hnRNP K is enriched in cytoplasmic membrane fractions following Sindbis virus infection, (ii) viral nonstructural proteins co-immunoprecipitate with hnRNP K, (iii) nsP2 and hnRNP K co-localize in the cytoplasm of Sindbis virus infected cells, (iv) Sindbis virus subgenomic mRNA, but not genomic RNA co-immunoprecipitates with hnRNP K, (v) viral RNA does not appear to be required for the interaction of hnRNP K with the nonstructural proteins. Potential functions of hnRNP K during virus replication are discussed

  2. Components production and assemble of the irradiation capsule of the Surveillance Program of Materials of the nuclear power plant of Laguna Verde

    International Nuclear Information System (INIS)

    Medrano, A.

    2009-01-01

    To predict the effects of the neutrons radiation and the thermal environment about the mechanical properties of the reactor vessel materials of the nuclear power plant of Laguna Verde, a surveillance program is implemented according to the outlines settled by Astm E185-02 -Standard practice for design of surveillance programs for light-water moderated nuclear power reactor vessels-. This program includes the installation of three irradiation capsules of similar materials to those of the reactor vessels, these samples are test tubes for mechanical practices of impact and tension. In the National Institute of Nuclear Research and due to the infrastructure as well as of the actual human resources of the Pilot Plant of Nuclear Fuel Assembles Production it was possible to realize the materials rebuilding extracted in 2005 of Unit 2 of nuclear power plant of Laguna Verde as well as the production, assemble and reassignment of the irradiation capsule made in 2006. At the present time the surveillance materials extracted in 2008 of Unit 1 of the nuclear power plant of Laguna Verde are reconstituting and the components are manufactured for the assembles of the irradiation capsule that will be reinstalled in the reactor vessel in 2010. The purpose of the present work is to describe the necessary components as well as its disposition during the assembles of the irradiation capsule for the surveillance program of the reactors vessel of the nuclear power plant of Laguna Verde. (Author)

  3. On-line surveillance system for Borssele nuclear power plant monitoring and diagnostics

    International Nuclear Information System (INIS)

    Tuerkcan, E.; Ciftcioglu, Oe.

    1993-08-01

    An operating on-line surveillance and diagnostic system is described where information processing for monitoring and fault diagnosis and plant maintenance are addressed. The surveillance system by means of its realtime multiprocessing, multitasking execution capabilities can perform plant-wide and wide-range monitoring for enhanced plant safety and operational reliability as well as enhanced maintenance. At the same time the system provides the possibilities for goal-oriented research and development such as estimation, filtering, verification and validation and neural networks. (orig./HP)

  4. Swiss regulatory use of databanks for nuclear power plant life management, surveillance and safety analyses

    International Nuclear Information System (INIS)

    Tipping, Ph.; Beutler, R.; Schoen, G.; Noeggerath, J.

    2002-01-01

    Full text: As operational time is accumulated, the overall safety and performance of nuclear power plants (NPPs) will tend to be characterised by those areas in which structures, systems and components (SSCs) have not performed as well, or as reliably, as expected. The reasons for non-availability of equipment in NPPs due to SSC material malfunction or unsatisfactory performance, leading to events or even accidents, are varied and they must be analysed in order to obtain the root causes. Once the root causes are identified, corresponding measures can be applied in order to improve reliability and therefore safety. The root cause information obtained, if brought into user-friendly databanks (DBs), can be used to follow NPP performance trends, to check whether a repair or replacement has been effective, to focus regulatory attention and NPP surveillance on known weak-spots and to serve as an advance indicator where potential problems may arise. Using the DBs, similar occurrences of failures or problems in other NPPs can be identified and generic issues recognised early on and preventative action taken. The following describes the Swiss Federal Nuclear Safety Inspectorate's (HSK) DB concepts for keeping track of NPP safety and lifetime management issues. Typical sources of data for the Inspectorate's DBs are, for example, the IAEA/NEA Incident Reporting System (IRS) reports, US-NRC Generic Letters, the Swiss NPP's own reports (monthly, annual and normal outage) and, more importantly, the document that these NPPs must issue to the Inspectorate whenever a reportable event takes place. Specifically, the reporting of events in the NPPs is laid down in the Inspectorate's Guideline (R-15 'Reporting Guideline Concerning The Operation of Nuclear Power Plants'). In this Guideline, reportable events are defined and the criteria for assessing the degree of importance or impact on nuclear safety are given. In this manner, a standard and consistent approach to data collection is

  5. Analysis of mechanical property data obtained from nuclear pressure vessel surveillance capsules

    International Nuclear Information System (INIS)

    Perrin, J.S.

    1977-01-01

    A typical pressure vessel surveillance capsule examination program provides mechanical property data from tensile, Charpy V-notch impact, and, in some cases, fracture mechanics specimens. This data must be analyzed in conjunction with the unirradiated baseline mechanical property data to determine the effect of irradiation on the mechanical properties. In the case of Charpy impact specimens, for example, irradiation typically causes an increase in the transition temperature, and a decrease in the upper shelf energy level. The results of the Charpy impact and other mechanical specimen tests must be evaluated to determine if property changes are occurring in the manner expected when the reactor was put into service. The large amount of data obtained from surveillance capsule examinations in recent years enables one to make fairly good predictions. After the changes in the mechanical properties of specimens from a particular surveillance capsule have been experimentally determined and evaluated, they must be related to the reactor pressure vessel. This requires a knowledge of the neutron fluence of the surveillance capsule, and the ratio of the surveillance capsule fluence to the pressure vessel wall fluence. This ratio is frequently specified by the reactor manufacturer, or can be calculated from a knowledge of the geometry and materials of the reactor components inside the pressure vessel. A knowledge of the exact neutron fluence of the capsule specimens and the capsule to vessel wall neutron fluence ratio is of great importance, since inaccuracies in these numbers cause just as serious a problem as inaccuracies in the mechanical property determinations. A further area causing analysis difficulties is problems encountered in recent capsule programs relating to capsule design, construction, operation, and dismantling. (author)

  6. Nuclear Power Plant environment`s surveillance by satellite remote sensing and in-situ monitoring data

    Science.gov (United States)

    Zoran, Maria

    The main environmental issues affecting the broad acceptability of nuclear power plant are the emission of radioactive materials, the generation of radioactive waste, and the potential for nuclear accidents. All nuclear fission reactors, regardless of design, location, operator or regulator, have the potential to undergo catastrophic accidents involving loss of control of the reactor core, failure of safety systems and subsequent widespread fallout of hazardous fission products. Risk is the mathematical product of probability and consequences, so lowprobability and high-consequence accidents, by definition, have a high risk. NPP environment surveillance is a very important task in frame of risk assessment. Satellite remote sensing data had been applied for dosimeter levels first time for Chernobyl NPP accident in 1986. Just for a normal functioning of a nuclear power plant, multitemporal and multispectral satellite data in complementarily with field data are very useful tools for NPP environment surveillance and risk assessment. Satellite remote sensing is used as an important technology to help environmental research to support research analysis of spatio-temporal dynamics of environmental features nearby nuclear facilities. Digital processing techniques applied to several LANDSAT, MODIS and QuickBird data in synergy with in-situ data are used to assess the extent and magnitude of radiation and non-radiation effects on the water, near field soil, vegetation and air. As a test case the methodology was applied for for Nuclear Power Plant (NPP) Cernavoda, Romania. Thermal discharge from nuclear reactors cooling is dissipated as waste heat in Danube-Black -Sea Canal and Danube River. Water temperatures captured in thermal IR imagery are correlated with meteorological parameters. If during the winter thermal plume is localized to an area of a few km of NPP, the temperature difference between the plume and non-plume areas being about 1.5 oC, during summer and fall , is

  7. Corrosion surveillance for research reactor spent nuclear fuel in wet basin storage

    International Nuclear Information System (INIS)

    Howell, J.P.

    1999-01-01

    Foreign and domestic test and research reactor fuel is currently being shipped from locations over the world for storage in water filled basins at the Savannah River Site (SRS). The fuel was provided to many of the foreign countries as a part of the ''Atoms for Peace'' program in the early 1950's. In support of the wet storage of this fuel at the research reactor sites and at SRS, corrosion surveillance programs have been initiated. The International Atomic Energy Agency (IAEA) established a Coordinated Research Program (CRP) in 1996 on ''Corrosion of Research Reactor Aluminum-Clad Spent Fuel in Water'' and scientists from ten countries worldwide were invited to participate. This paper presents a detailed discussion of the IAEA sponsored CRP and provides the updated results from corrosion surveillance activities at SRS. In May 1998, a number of news articles around the world reported stories that microbiologically influenced corrosion (MIC) was active on the aluminum-clad spent fuel stored in the Receiving Basin for Offsite Fuels (RBOF) at SRS. This assessment was found to be in error with details presented in this paper. A biofilm was found on aluminum coupons, but resulted in no corrosion. Cracks seen on the surface were not caused by corrosion, but by stresses from the volume expansion of the oxide formed during pre-conditioning autoclaving. There has been no pitting caused by MIC or any other corrosion mechanism seen in the RBOF basin since initiation of the SRS Corrosion Surveillance Program in 1993

  8. Comparison of Various Nuclear Localization Signal-Fused Cas9 Proteins and Cas9 mRNA for Genome Editing in Zebrafish.

    Science.gov (United States)

    Hu, Peinan; Zhao, Xueying; Zhang, Qinghua; Li, Weiming; Zu, Yao

    2018-03-02

    The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has been proven to be an efficient and precise genome editing technology in various organisms. However, the gene editing efficiencies of Cas9 proteins with a nuclear localization signal (NLS) fused to different termini and Cas9 mRNA have not been systematically compared. Here, we compared the ability of Cas9 proteins with NLS fused to the N-, C-, or both the N- and C-termini and N-NLS-Cas9-NLS-C mRNA to target two sites in the tyr gene and two sites in the gol gene related to pigmentation in zebrafish. Phenotypic analysis revealed that all types of Cas9 led to hypopigmentation in similar proportions of injected embryos. Genome analysis by T7 Endonuclease I (T7E1) assays demonstrated that all types of Cas9 similarly induced mutagenesis in four target sites. Sequencing results further confirmed that a high frequency of indels occurred in the target sites ( tyr1 > 66%, tyr2 > 73%, gol1 > 50%, and gol2 > 35%), as well as various types (more than six) of indel mutations observed in all four types of Cas9-injected embryos. Furthermore, all types of Cas9 showed efficient targeted mutagenesis on multiplex genome editing, resulting in multiple phenotypes simultaneously. Collectively, we conclude that various NLS-fused Cas9 proteins and Cas9 mRNAs have similar genome editing efficiencies on targeting single or multiple genes, suggesting that the efficiency of CRISPR/Cas9 genome editing is highly dependent on guide RNAs (gRNAs) and gene loci. These findings may help to simplify the selection of Cas9 for gene editing using the CRISPR/Cas9 system. Copyright © 2018 Hu et al.

  9. Surveillance and analysis of nuclear R and D manpower for efficient utilization of KAERI's nuclear R and D resources

    International Nuclear Information System (INIS)

    Kim, Hyun Jun; Yang, M. H.; Song, K. D.; Chung, W. S.; Yun, S. W.; Won, B. C.; Yoon, H. M.

    1999-11-01

    This study aimed at securing row data on KAERI's manpower resources, which be useful to establish the short and long term R and D policy of KAERI. To coincide with the objectives, this study covered following scopes; (1) identification of status and issues of manpower in science and technology fields, (2) identification of status and issues of KAERI's manpower management policy, (3) gathering row data on KAERI's manpower through surveillance and analysis, and analyzing staff's perception on KAERI's manpower utilization policy, (4) suggestion of short and long term policy direction of manpower management of KAERI. (author)

  10. Studies for transitional changes of the muscarinic acetylcholine receptor and mRNA distribution by focal ischemia using nuclear medicine

    Energy Technology Data Exchange (ETDEWEB)

    Kuji, Ichiei [Kanazawa Univ. (Japan). School of Medicine

    1994-04-01

    Assessing stress-induced brain receptor responses is important in understanding clinical brain receptor images for nuclear medicine. It is known that cholinergic neurons are decreased by Alzheimer`s disease and that there is a close relationship between cholinergic neurons and muscarinic acetylcholine receptors (mAchR). Thus, this study assessed the response of mAchR to focal ischemia using infarction model rats (prepared by middle cerebral artery occlusion) and sham-operated rats. In the same rats, three kinds of images -- ex vivo regional cerebral blood flow (rCBF) images with {sup 99m}Tc-hexametyl-propyleneamine oxime ({sup 99m}Tc-HMPAO), in vitro mAchR binding images with [{sup 3}H] quinuclidinyl benzilate ({sup 3}H-QNB), and mAchR-mRNA images by in situ hybridization method using {sup 35}S-labeled-oligonucleotide probes specific for mAchR gene subtypes of m1 to m5 -- were obtained in acute and chronic phases. Each image datum was digitalized and assessed semi-quantitatively. There were significant changes in global distribution among rCBF, mAchR and mAchR-mRNAs. In the acute phase, there was no significant change in mAchR in the infarcted area, although rCBF markedly decreased. In the chronic phase, there was a significant decrease in mAchR in the infarct-sided thalamus, although there was no change in rCBF; and there was a significant decrease in mAchR of the infarct-sided substantia nigra in spite of increase in rCBF. In the acute phase, mAchR-mRNAs of the infarct-sided caudate-putamen was decreased, suggesting that the ability of cholinergic neuron to synthesize receptor protein had decreased in the acute phase. Because mAchR was not decreased in the acute phase, some viable neurons with no normal function may be preserved in the acute phase. These results were encouraging in understanding mAchR brain images of patients with memory disturbances such as cerebrovascular dementia and Alzheimer`s disease. (N.K.).

  11. Automated long-term surveillance of a commercial nuclear power plant

    International Nuclear Information System (INIS)

    Smith, C.M.; Gonzalez, R.C.

    1987-08-01

    This report presents and describes a pattern recognition system for monitoring nuclear reactor signals. The system is based on detecting deviations from baseline signatures identified during normal plant operation. The capabilities and limitations of this pattern recognition approach were investigated during a 2-1/2-year series of continuous online experiments at the Sequoyah-1 Nuclear Power Plant

  12. Long-term automated surveillance of a commercial nuclear power plant

    International Nuclear Information System (INIS)

    Smith, C.M.; Gonzalez, R.C.

    1984-01-01

    A pattern recognition system for monitoring nuclear reactor signals is presented. The system is based on detecting deviations from baseline signatures learned automatically during normal plant operation. The capabilities and limitations of the recognition approach were investigated during a 2-1/2-year continuous, on-line series of experiments at the Sequoyah-1 nuclear power plant

  13. Requirements and concepts for a nuclear plant surveillance and diagnostic system (NPSDS)

    International Nuclear Information System (INIS)

    Nicholson, P.J.; Lanning, D.D.

    1980-01-01

    An advanced plant surveillance and diagnostic system has been postulated for the purpose of aiding operator response to unanticipated plant upsets. The plant operator is described as an information processor that needs plant status information represented by symbolic outputs. These will be compatible with modern visual processing techniques, such as CRTs. Preferrred methods of estimating the state-of-the-plant and verifying measurements require on-line real-time models which are simple dynamical relationships based on energy and mass conservation laws. Implementation of on-line state estimation techniques using such models probably requires a distributed microcomputer system whose features are described. (auth)

  14. A niching genetic algorithm applied to a nuclear power plant auxiliary feedwater system surveillance tests policy optimization

    International Nuclear Information System (INIS)

    Sacco, W.F.; Lapa, Celso M.F.; Pereira, C.M.N.A.; Oliveira, C.R.E. de

    2006-01-01

    This article extends previous efforts on genetic algorithms (GAs) applied to a nuclear power plant (NPP) auxiliary feedwater system (AFWS) surveillance tests policy optimization. We introduce the application of a niching genetic algorithm (NGA) to this problem and compare its performance to previous results. The NGA maintains a populational diversity during the search process, thus promoting a greater exploration of the search space. The optimization problem consists in maximizing the system's average availability for a given period of time, considering realistic features such as: (i) aging effects on standby components during the tests; (ii) revealing failures in the tests implies on corrective maintenance, increasing outage times; (iii) components have distinct test parameters (outage time, aging factors, etc.) and (iv) tests are not necessarily periodic. We find that the NGA performs better than the conventional GA and the island GA due to a greater exploration of the search space

  15. Ensuring preservation of nuclear materials at RT plant storage facilities by upgrading physical protection, containment (TID) and TV surveillance measures

    International Nuclear Information System (INIS)

    Savin, A.A.

    1999-01-01

    The upgrading of nuclear material (NM) physical protection system of storage facilities for plutonium dioxide has been carried out in the frame of contract between Sandia National Laboratories and PA Mayak. The implementation of this project is of great importance for ensuring the preservation of NM in storage. The project envisages a complex approach to the solution of the task: the use of engineered fences, detection means, observation devices, access and control thereof for the personnel; the organization of security force in such way that to give a possibility to take adequate and purposeful actions towards possible adversaries. The design includes upgrading the system for physical protection through the introduction of modern, efficient engineering devices for security, integrated system for access control and security, the system for TV surveillance [ru

  16. Fracture mechanics investigations within the swiss surveillance programme for the pressure vessel of modern nuclear power plants

    Energy Technology Data Exchange (ETDEWEB)

    Ullrich, G; Krompholz, K [Paul Scherrer Inst. (PSI), Villigen (Switzerland)

    1994-12-31

    In the frame of surveillance programmes of Swiss nuclear power plants, irradiation tests have been performed on tensile, impact and wedge opening load specimens as well as on three point bend-type specimens (for J-integral investigations) and pre-cracked Charpy impact specimens (for dynamical stress intensities K{sub ID}). An experimental method (potential drop technique) is used together with a mathematical procedure which allow for the determination of the stress intensity K{sub IC} for small CT-samples instead of large ones: agreement of these both methods is found excellent, and the mapping of both methods to fatigue pre-cracked small specimens (3 PB and Charpy) is possible. The application of the analysis method to dynamical tests is also possible. 15 refs., 9 figs., 1 tab.

  17. Accountability and non-proliferation nuclear regime: a review of the mutual surveillance Brazilian-Argentine model for nuclear safeguards

    International Nuclear Information System (INIS)

    Xavier, Roberto Salles

    2014-01-01

    The regimes of accountability, the organizations of global governance and institutional arrangements of global governance of nuclear non-proliferation and of Mutual Vigilance Brazilian-Argentine of Nuclear Safeguards are the subject of research. The starting point is the importance of the institutional model of global governance for the effective control of non-proliferation of nuclear weapons. In this context, the research investigates how to structure the current arrangements of the international nuclear non-proliferation and what is the performance of model Mutual Vigilance Brazilian-Argentine of Nuclear Safeguards in relation to accountability regimes of global governance. For that, was searched the current literature of three theoretical dimensions: accountability, global governance and global governance organizations. In relation to the research method was used the case study and the treatment technique of data the analysis of content. The results allowed: to establish an evaluation model based on accountability mechanisms; to assess how behaves the model Mutual Vigilance Brazilian-Argentine Nuclear Safeguards front of the proposed accountability regime; and to measure the degree to which regional arrangements that work with systems of global governance can strengthen these international systems. (author)

  18. Surveillance of a nuclear reactor by use of a pattern recognition methodology

    International Nuclear Information System (INIS)

    Dubuisson, B.; Lavison, P.

    1980-01-01

    A multivariate nonparametric pattern recognition system is described for the surveillance of a high-flux isotope reactor. Two nonparametric methods are worked out: one using the Bayes rule with the Rosenblatt-Parzen estimator for the probability law, and one using the k-nearest neighbor rule. Performances are evaluated by comparing the probability of misclassification between the two chosen classes: the first corresponds to a nonaction of the reactor operator on its power and the second to an action of the pilot. Processing is performed on the power signal of the reactor which is an observation corrupted by noise. The system has been tested on several experiences and implemented to work in real time on the reactor. The aim is to conceive a computer-aided decision system for the reactor's pilot. 17 refs

  19. Monitoring around the secret nuclear facilities of naval ports; Surveillance autour des INBS des ports militaires

    Energy Technology Data Exchange (ETDEWEB)

    Jaskierowicz, D. [Marine Nationale, pharmacien en chef, conseiller scientifique et technique, etat-major de la marine, 83 - Toulon (France); Quere, St. [Marine Nationale, capitaine de corvette, adjoint charge de la prise en compte des installations nucleaires sur l' environnement et le personnel, 83 - Toulon (France)

    2010-06-15

    Based within large industrial cities (Brest, Toulon, Cherbourg) or more rural areas like Crozon (Ile Longue), French navy exploits nuclear facilities where are built, maintained and decommissioned nuclear power submarines and aircraft-carrier. The safety and the security of these installations as well as the non-impact on people and environment are continuously monitored. The DSND, a governmental regulatory body dedicated to the Defense, applies the same regulations enforced by the ASN for civilian nuclear activities. Concerning environmental monitoring, the navy answers to the DSND or the ASN, depending on the type of survey. In every nuclear site, an automatic nuclear monitoring sensor system (2SNM) runs 24/7, with the supervision of specialized personnel in radioprotection. Each year, more than 7000 samples are collected in the ecosystem and thousands of measurements are carried out in four laboratories (LASEM in Cherbourg, Brest and Toulon) - SPRS ILO) of the navy. These results are sent to the DSND and have been integrated since February 2010 to the brand-new public web site of the national monitoring network of radioactivity in the environment (RMN). (author)

  20. Quality surveillance for steel forgings of SA508 Gr.3 used on the main NI equipment of AP1000 nuclear island

    International Nuclear Information System (INIS)

    Liu Lizhao

    2011-01-01

    Being a type of steel with ideal weldability, outstanding ability of anti-neutron irradiation embitterment and good property of fracture toughness and impact toughness, the steel of ASME SA508-3 was used widely for the nuclear island equipment of PWR Nuclear Power Plant. For the 3rd generation nuclear power plant AP1000, all large forgings and some critical components of the SG, RV and PRZ adopt this steel. Through analysis on the critical technical points during manufacturing of the SA508-3 forgings, this article try to identify the key points should be paid attention during the quality surveillance for this type of forgings, and to put forward the supervision method and focus during quality surveillance activities. (author)

  1. Use of bioindicators in the environmental radiological surveillance of Mexican Nuclear Center

    International Nuclear Information System (INIS)

    Gaso P, M.I.; Segovia, N.; Herrera, T.; Perez S, E.; Cervantes, M.L.; Quintero, E.; Palacios, J.; Acosta, E.; Segovia, N.; Herrera, T.

    1998-01-01

    Cs-137 and K-40 concentrations were determined in soil samples and wild eatable mushrooms at the forest ecosystem of Mexican Nuclear Center and its surrounding area. The evaluation was carried out with a low bottom gamma spectroscopy system including a high-purity Ge detector (HPGe). The local species that showed greater soil-mushroom transference factor, for Cs-137, were Clavariadelphus truncatus, Cortinarius caerulescenc, Gomphus floccosus and Lyophyllum decastes. The Cs-137 levels obtained in the mushroom samples of Nuclear Center, were in general slightly lower than those of adjacent places, which indicates that this Center is not polluting the atmosphere with such radionuclide. (Author)

  2. Radiological surveillance employed at industrial application in a nuclear research centre

    International Nuclear Information System (INIS)

    Sanches, M.P.; Sordi, G.M.; Sahyun, A.; Rodrigues, D.L.

    1996-01-01

    The monitoring and dosimetry systems used at Industrial Application and Engineering Service Department of the Instituto de Pesquisas Energeticas e Nucleares of the Brazilian Nuclear Energy Commission (GE-IPEN-CNEN/SP) are analyzed,in order to verify the compliance with the condition established by the dose system limitation and the basic radiation protection standards. The criteria about the use of individual dosimetry for persons that work in these facilities are established. In case of an external radiation dosimetry, by using film badge method, a level of 200μGy has been assigned, considering the detection significant threshold. For dose in air due to electromagnetic radiation, using TLD dosimetry of CaSO 4 :Dy a quarter year significant threshold is 101μGy. A symbiosis between the workplace monitoring and the individual monitoring became necessary for optimization purpose. (authors). 5 refs., 2 tabs., 1 fig

  3. Pattern recognition application for surveillance of abnormal conditions in a nuclear reactor

    International Nuclear Information System (INIS)

    Pepelyshev, Yu.N.; Dzwinel, W.

    1990-01-01

    The system to monitor abnormal conditions in a nuclear reactor, based on the noise analysis of the reactor basic parameters such as power, temperature and coolant flow rate, has been developed. The pattern recognition techniques such as clustering, cluster analysis, feature selection and clusters visualization methods form the basis of the software. Apart from non-hierarchical clustering procedures applied earlier, the hierarchical one is recommended. The system application for IBR-2 Dubna reactor diagnostics is shown. 10 refs.; 6 figs

  4. Regulatory surveillance of safety related maintenance at nuclear power plants. Report of a technical committee meeting

    International Nuclear Information System (INIS)

    1997-08-01

    The operational safety and reliability of a nuclear power plant as well as its availability for electricity generation depend on, among other things, its maintenance programme. Regulatory bodies therefore have considerable interest in maintenance activities. There are several approaches to maintenance, i.e. reliability centered maintenance or risk focused maintenance, aimed at optimizing maintenance by focusing on important components or systems. These approaches may result in significant changes to maintenance activities and therefore have to be considered for regulatory acceptance. In order to review and discuss the status of maintenance regulation in participating countries, the IAEA convened a Technical Committee Meeting on Regulatory Oversight of Maintenance Activities at Nuclear Power Plants in Vienna from 9 to 13 October 1995. The meeting was attended by 16 experts from 11 countries. In addition to the consideration of papers that were presented and which are reproduced here, extensive group and panel discussions took place during the meeting. These covered three main topics: general features and basic characteristics of maintenance regulation, regulatory acceptance of maintenance optimization and use of PSA for maintenance optimization. The discussion are summarized in Section 2. Section 3 discusses the following three additional topics: regulatory involvement in the maintenance programme, modifications to the maintenance programme and personnel related aspects of maintenance. The conclusions are presented in Section 4. Figs, tabs

  5. Ground-based remote sensing profiling and numerical weather prediction model to manage nuclear power plants meteorological surveillance in Switzerland

    Directory of Open Access Journals (Sweden)

    B. Calpini

    2011-08-01

    Full Text Available The meteorological surveillance of the four nuclear power plants in Switzerland is of first importance in a densely populated area such as the Swiss Plateau. The project "Centrales Nucléaires et Météorologie" CN-MET aimed at providing a new security tool based on one hand on the development of a high resolution numerical weather prediction (NWP model. The latter is providing essential nowcasting information in case of a radioactive release from a nuclear power plant in Switzerland. On the other hand, the model input over the Swiss Plateau is generated by a dedicated network of surface and upper air observations including remote sensing instruments (wind profilers and temperature/humidity passive microwave radiometers. This network is built upon three main sites ideally located for measuring the inflow/outflow and central conditions of the main wind field in the planetary boundary layer over the Swiss Plateau, as well as a number of surface automatic weather stations (AWS. The network data are assimilated in real-time into the fine grid NWP model using a rapid update cycle of eight runs per day (one forecast every three hours. This high resolution NWP model has replaced the former security tool based on in situ observations (in particular one meteorological mast at each of the power plants and a local dispersion model. It is used to forecast the dynamics of the atmosphere in the planetary boundary layer (typically the first 4 km above ground layer and over a time scale of 24 h. This tool provides at any time (e.g. starting at the initial time of a nuclear power plant release the best picture of the 24-h evolution of the air mass over the Swiss Plateau and furthermore generates the input data (in the form of simulated values substituting in situ observations required for the local dispersion model used at each of the nuclear power plants locations. This paper is presenting the concept and two validation studies as well as the results of an

  6. Risk-based evaluation of allowed outage time and surveillance test interval extensions for nuclear power plants

    International Nuclear Information System (INIS)

    Gibelli, Sonia Maria Orlando

    2008-03-01

    The main goal of this work is, through the use of Probabilistic Safety Analysis (PSA), to evaluate Technical Specification (TS) Allowed Outage Times (AOT) and Surveillance Test Intervals (STI) extensions for Angra 1 nuclear power plant. PSA has been incorporated as an additional tool, required as part of NPP licensing process. The risk measure used in this work is the Core Damage Frequency (CDF), obtained from the Angra 1 PSA Level 1. AOT and STI extensions are calculated for the Safety Injection System (SIS), Service water System (SAS) and Auxiliary Feedwater System (AFS) through the use of SAPHIRE code. In order to compensate for the risk increase caused by the extensions, compensatory measures as test of redundant train prior to entering maintenance and staggered test strategy are proposed. Results have shown that the proposed AOT extensions are acceptable for the SIS and SAS with the implementation of compensatory measures. The proposed AOT extension is not acceptable for the AFS. The STI extensions are acceptable for all three systems. (author)

  7. Parallel island genetic algorithm applied to a nuclear power plant auxiliary feedwater system surveillance tests policy optimization

    International Nuclear Information System (INIS)

    Pereira, Claudio M.N.A.; Lapa, Celso M.F.

    2003-01-01

    In this work, we focus the application of an Island Genetic Algorithm (IGA), a coarse-grained parallel genetic algorithm (PGA) model, to a Nuclear Power Plant (NPP) Auxiliary Feedwater System (AFWS) surveillance tests policy optimization. Here, the main objective is to outline, by means of comparisons, the advantages of the IGA over the simple (non-parallel) genetic algorithm (GA), which has been successfully applied in the solution of such kind of problem. The goal of the optimization is to maximize the system's average availability for a given period of time, considering realistic features such as: i) aging effects on standby components during the tests; ii) revealing failures in the tests implies on corrective maintenance, increasing outage times; iii) components have distinct test parameters (outage time, aging factors, etc.) and iv) tests are not necessarily periodic. In our experiments, which were made in a cluster comprised by 8 1-GHz personal computers, we could clearly observe gains not only in the computational time, which reduced linearly with the number of computers, but in the optimization outcome

  8. Artificial Intelligence application to surveillance and diagnosis of nuclear power plants

    International Nuclear Information System (INIS)

    Brunet, E.; Monnier, B.; Zwingelstein, G.

    1986-01-01

    Acquisition and representation of knowledge are fundamental problems in Aritificial Intellegence and especially in expert system domain. In this article, the authors propose a conceptual model allowing to describe the universe the expert is working on when trying to make a diagnosis. The expert determines the descriptors and predicates with which he describes laws, states, objects involved in his reasoning. With this model, they are presenting design and development of Knowledge Acquisition Module allowing a dialogue in a specialized natural language used by the expert system to assist in the detection of loose parts in nuclear power plants. Using a classical lexical and syntactic analysis, they propose to associate grammatical semantic properties to the specialized language words. The method used allows the design of a sub expert system for understanding of the specialized natural language

  9. Design of quality assurance surveillance of geotechnical investigation in evaluation of nuclear power plants siting

    International Nuclear Information System (INIS)

    Made Pramayuni; Haendra Subekti

    2011-01-01

    System of building Nuclear Power Plants (NPP) is built above ground surface and rocks. Therefore, the geotechnical aspects evaluation of NPP siting in particular must be implemented properly and accurately. The successful of the design and construction of the building system is influenced by geotechnical conditions in the vicinity of NPP will be built. To be able to perform geotechnical analysis is good and true; it's required data of surface/underground completely. These data can be obtained directly not only from the geotechnical field investigations, but also can be obtained from the laboratory tests results. To ensure that geotechnical investigations are conducted effectively and efficiently, produce accurate data, as well as meeting the requirements of safety, health, safety, environmental, quality and economic, then the Quality Assurance Program (QAP) should be established and implemented. Supervision of the QAP implementation is required to ensure the work is done according to QAP that have been established and applicable requirement. (author)

  10. Annual monitoring and surveillance report for Piqua Nuclear Power Facility, Piqua, Ohio

    International Nuclear Information System (INIS)

    Mosho, G.D.

    1991-12-01

    This report discusses the decommissioned Piqua Nuclear Power Facility which is located in Piqua, Ohio near the Greater Miami River. The Facility was built by the US Atomic Energy Commission (now U. S. Department of Energy) and was operated from 1963 to 1966. The reactor was retired prior to 1970 and the facility was leased to the city of Piqua for use as offices and equipment storage. In December 1991, a radiological survey was done of the facility to document its radiological condition. The data show that all radiological parameters measured were essentially the same as that found in the natural environment. The only exception was that low levels of radioactive contamination were detected in one drain on the 56.5 ft elevation, but the radiation exposure rate in that area was also typical of natural background

  11. The particle swarm optimization algorithm applied to nuclear systems surveillance test planning

    International Nuclear Information System (INIS)

    Siqueira, Newton Norat

    2006-12-01

    This work shows a new approach to solve availability maximization problems in electromechanical systems, under periodic preventive scheduled tests. This approach uses a new Optimization tool called PSO developed by Kennedy and Eberhart (2001), Particle Swarm Optimization, integrated with probabilistic safety analysis model. Two maintenance optimization problems are solved by the proposed technique, the first one is a hypothetical electromechanical configuration and the second one is a real case from a nuclear power plant (Emergency Diesel Generators). For both problem PSO is compared to a genetic algorithm (GA). In the experiments made, PSO was able to obtain results comparable or even slightly better than those obtained b GA. Therefore, the PSO algorithm is simpler and its convergence is faster, indicating that PSO is a good alternative for solving such kind of problems. (author)

  12. Corrosion surveillance of the chemical decontamination process in Kuosheng nuclear power plant

    International Nuclear Information System (INIS)

    Wang, L.H.

    2002-01-01

    The Piping Recirculation System (RRS) and reactor water clean-up system (RWCU) of Kuosheng Nuclear Power Plant of Taiwan Power Company were decontaminated by CORD process of Framatome ANP GmbH during the outage at October 2001. This is the first time that CORD process was adopted and applied in Taiwan Nuclear Power Plant. To verify minor corrosion damage and correct process control, the material corrosion condition was monitored during all the stages of the chemical decontamination work. Three kinds of specimen were adopted in this corrosion monitoring, including corrosion coupons for weight loss measurements, electrochemical specimens for on-line corrosion monitoring, and WOL specimens (wedge opening loaded) for stress corrosion evaluation. The measured metal losses from nine coupon materials did not reveal any unexpected or intolerable high corrosion damage from the CORD UV or CORD CS processes. The coupon materials included type 304 stainless steel (SS) with sensitized and as-received thermal history, type 308 weld filler, type CF8 cast SS, nickel base alloy 182 weld filler, Inconel 600, Stellite 6 hard facing alloy, NOREM low cobalt hard facing alloy, and A106B carbon steel (CS). The electrochemical noise (ECN) measurements from three-electrode electrochemical probe precisely depicted the metal corrosion variation with the decontamination process change. Most interestingly, the estimated trend of accumulated metal loss is perfectly corresponding to the total removed activities. The ECN measurements were also used for examining the effect of different SS oxide films pre-formed in NWC and HWC on the decontamination efficiency, and for evaluating the galvanic effect of CS with SS. The existing cracks did not propagate further during the decontamination. The average decontamination factors achieved were 50.8 and 4.2 respectively for RRS and RWCU. (authors)

  13. Studies and development of essential systems in the surveillance program, life extension potential of the vessel and master curve in nuclear power plants

    International Nuclear Information System (INIS)

    Romero C, J.; Hernandez C, R.; Rocamontes A, M.; Perez R, N.

    2010-01-01

    The nuclear power plants owners should demonstrate that the effects of the embrittlement by neutronic radiation do not commit the structural integrity of the pressure vessel of the nuclear reactors, so much under conditions of routine operation as below an accident postulate. In consequence, in Mexico surveillance programs of the vessels of the nuclear power plant of Laguna Verde exist, in which three surveillance capsules are have by reactor. A surveillance capsule is composed by a support and between six and eight containers for test tubes and dosemeters. The containers for test tubes are of two types: rectangular container for Charpy V test tubes and cylindrical container for tension test tubes. These test tubes are subject to a same or bigger neutronic flow that of the vessel, being representative witness of the mechanical conditions of the vessel. The objective of to assay the test tubes to impact is to evaluate the embrittlement grade of the vessel beforehand during its useful life of operation, as well as to determinate the running of the ductile-fragile transition temperature in function of the time. (Author)

  14. Surveillance of Strontium-90 in Foods after the Fukushima Daiichi Nuclear Power Plant Accident.

    Science.gov (United States)

    Nabeshi, Hiromi; Tsutsumi, Tomoaki; Uekusa, Yoshinori; Hachisuka, Akiko; Matsuda, Rieko; Teshima, Reiko

    2015-01-01

    As a result of the Fukushima Daiichi nuclear power plant (NPP) accident, various radionuclides were released into the environment. In this study, we surveyed strontium-90 ((90)Sr) concentrations in several foodstuffs. Strontium-90 is thought to be the third most important residual radionuclide in food collected after the Fukushima Daiichi, NPP accident after following cesium-137 ((137)Cs) and cesium-134 ((134)Cs). Results of (90)Sr analyses indicated that (90)Sr was detect in 25 of the 40 radioactive cesium (r-Cs) positive samples collected in areas around the Fukushima Daiichi NPP, ranging in distance from 50 to 250 km. R-Cs positive samples were defined as containing both (134)Cs and (137)Cs which are considered to be indicators of the after-effects of the Fukushima Daiichi NPP accident. We also detected (90)Sr in 8 of 13 r-Cs negative samples, in which (134)Cs was not detected. Strontium-90 concentrations in the r-Cs positive samples did not significantly exceed the (90)Sr concentrations in r-Cs negative samples or the (90)Sr concentration ranges in comparable food groups found in previous surveys before the Fukushima Daiichi NPP accident. Thus, (90)Sr concentrations in r-Cs positive samples were indistinguishable from the background (90)Sr concentrations arising from global fallout prior to the Fukushima accident, suggesting that no marked increase of (90)Sr concentrations has occurred in r-Cs positive samples as a result of the Fukushima Daiichi NPP accident.

  15. Survival and Intra-Nuclear Trafficking of Burkholderia pseudomallei: Strategies of Evasion from Immune Surveillance?

    Directory of Open Access Journals (Sweden)

    Jamuna Vadivelu

    2017-01-01

    Full Text Available During infection, successful bacterial clearance is achieved via the host immune system acting in conjunction with appropriate antibiotic therapy. However, it still remains a tip of the iceberg as to where persistent pathogens namely, Burkholderia pseudomallei (B. pseudomallei reside/hide to escape from host immune sensors and antimicrobial pressure.We used transmission electron microscopy (TEM to investigate post-mortem tissue sections of patients with clinical melioidosis to identify the localisation of a recently identified gut microbiome, B. pseudomallei within host cells. The intranuclear presence of B. pseudomallei was confirmed using transmission electron microscopy (TEM of experimentally infected guinea pig spleen tissues and Live Z-stack, and ImageJ analysis of fluorescence microscopy analysis of in vitro infection of A549 human lung epithelial cells.TEM investigations revealed intranuclear localization of B. pseudomallei in cells of infected human lung and guinea pig spleen tissues. We also found that B. pseudomallei induced actin polymerization following infection of A549 human lung epithelial cells. Infected A549 lung epithelial cells using 3D-Laser scanning confocal microscopy (LSCM and immunofluorescence microscopy confirmed the intranuclear localization of B. pseudomallei.B. pseudomallei was found within the nuclear compartment of host cells. The nucleus may play a role as an occult or transient niche for persistence of intracellular pathogens, potentially leading to recurrrent episodes or recrudescence of infection.

  16. Survival and Intra-Nuclear Trafficking of Burkholderia pseudomallei: Strategies of Evasion from Immune Surveillance?

    Science.gov (United States)

    Vadivelu, Jamuna; Vellasamy, Kumutha Malar; Thimma, Jaikumar; Mariappan, Vanitha; Kang, Wen-Tyng; Choh, Leang-Chung; Wong, Kum Thong

    2017-01-01

    Background During infection, successful bacterial clearance is achieved via the host immune system acting in conjunction with appropriate antibiotic therapy. However, it still remains a tip of the iceberg as to where persistent pathogens namely, Burkholderia pseudomallei (B. pseudomallei) reside/hide to escape from host immune sensors and antimicrobial pressure. Methods We used transmission electron microscopy (TEM) to investigate post-mortem tissue sections of patients with clinical melioidosis to identify the localisation of a recently identified gut microbiome, B. pseudomallei within host cells. The intranuclear presence of B. pseudomallei was confirmed using transmission electron microscopy (TEM) of experimentally infected guinea pig spleen tissues and Live Z-stack, and ImageJ analysis of fluorescence microscopy analysis of in vitro infection of A549 human lung epithelial cells. Results TEM investigations revealed intranuclear localization of B. pseudomallei in cells of infected human lung and guinea pig spleen tissues. We also found that B. pseudomallei induced actin polymerization following infection of A549 human lung epithelial cells. Infected A549 lung epithelial cells using 3D-Laser scanning confocal microscopy (LSCM) and immunofluorescence microscopy confirmed the intranuclear localization of B. pseudomallei. Conclusion B. pseudomallei was found within the nuclear compartment of host cells. The nucleus may play a role as an occult or transient niche for persistence of intracellular pathogens, potentially leading to recurrrent episodes or recrudescence of infection. PMID:28045926

  17. Situation of the environmental surveillance and situation of the water table and rivers labelling around nuclear sites and old radioactive waste storages. Report for the high committee for the transparency and information on nuclear safety

    International Nuclear Information System (INIS)

    2008-01-01

    The High Committee for the openness and information on nuclear safety (H.C.T.I.S.N.) requested a study at I.R.S.N. concerning the situation of the surveillance of media and their quality and the diffusion of this information near the public, the identification of ground water or rivers that would present a radiological or chemical labelling, the link of these elements with the future national network of the radioactivity measurement in environment. This assessment must also allow to take stock of the situation relative to the surveillance of the quality of ground water that flow out of the level of old radioactive waste storages, especially registered in the ANDRA inventory. I.R.S.N. chose to limit its contribution: to the sites housing nuclear base installations and nuclear base installations that have been classified secret that come under the Minister in charge of energy; to old radioactive wastes storages located in these installations. (N.C.)

  18. hnRNP A2/B1 interacts with influenza A viral protein NS1 and inhibits virus replication potentially through suppressing NS1 RNA/protein levels and NS1 mRNA nuclear export

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Yimeng; Zhou, Jianhong; Du, Yuchun, E-mail: ydu@uark.edu

    2014-01-20

    The NS1 protein of influenza viruses is a major virulence factor and exerts its function through interacting with viral/cellular RNAs and proteins. In this study, we identified heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) as an interacting partner of NS1 proteins by a proteomic method. Knockdown of hnRNP A2/B1 by small interfering RNA (siRNA) resulted in higher levels of NS vRNA, NS1 mRNA, and NS1 protein in the virus-infected cells. In addition, we demonstrated that hnRNP A2/B1 proteins are associated with NS1 and NS2 mRNAs and that knockdown of hnRNP A2/B1 promotes transport of NS1 mRNA from the nucleus to the cytoplasm in the infected cells. Lastly, we showed that knockdown of hnRNP A2/B1 leads to enhanced virus replication. Our results suggest that hnRNP A2/B1 plays an inhibitory role in the replication of influenza A virus in host cells potentially through suppressing NS1 RNA/protein levels and NS1 mRNA nucleocytoplasmic translocation. - Highlights: • Cellular protein hnRNP A2/B1 interacts with influenza viral protein NS1. • hnRNP A2/B1 suppresses the levels of NS1 protein, vRNA and mRNA in infected cells. • hnRNP A2/B1 protein is associated with NS1 and NS2 mRNAs. • hnRNP A2/B1 inhibits the nuclear export of NS1 mRNAs. • hnRNP A2/B1 inhibits influenza virus replication.

  19. Development of High Temperature Chemistry Measurement System for Establishment of On-Line Water Chemistry Surveillance Network in Nuclear Power Plant

    International Nuclear Information System (INIS)

    Yeon, Jei Won; Kim, Won Ho; Song, Kyu Seok; Joo, Ki Soo; Choi, Ke Chon; Ha, Yeong Keong; Ahn, Hong Joo; Im, Hee Jung; Maeng, Wan Young

    2010-07-01

    An integrated high-temperature water chemistry sensor (pH, E redox ) was developed for the establishment of the on-line water chemistry surveillance system in nuclear power plants. The basic performance of the integrated sensor was confirmed in high-temperature (280 .deg. C, 150kg/m 2 ) lithium borate solutions by using the relationship between the concentration of lithium ion and pH-E redox values. Especially, the effects of various environmental factors such as temperature, pressure, and flow rate on YSZ-based pH electrode were evaluated for ensuring the accuracy of high-temperature pH measurement. And the relationships between each water chemistry factor (pH, redox potential, electrical conductivity) were induced for enhancing the credibility of water chemistry measurement. In addition, on the basis of the evaluation of a nuclear plant design company, we suggested potential installation positions of the measurement system in a nuclear power plant

  20. Social safety and medical maintenance of the labour pool in surveillance areas of the nuclear power plants.

    Science.gov (United States)

    Prylipko, V A; Ozerova, Yu Yu; Kotsubinskij, O V; Morozova, M M; Petrychenko, O O; Bondarenko, I V

    2017-12-01

    To study the contentment of population of NPP surveillance areas i.e. monitoring zones with specific components of quality of life, namely the social security, medical care, and socio economic compensation of risk. A sociological study of public opinion about the specific components of quality of life has been conducted in population of the NPP monitoring zone. A questionnaire with independent question blocks was developed. A non repeatable probabilistic selection was applied in population opinion poll. The sampled population was calculated on the basis of the total population living in the NPP monitoring zone. Sample error not exceeded 7.0%. A comparative assessment of the responses of various groups of the able bodied population on issues of social security, medical care, socio economic compensation of risk and analysis of statistical data for 2011-2015 on the resource potential of medical facility of the nuclear power plant overspill town has been conducted. The safety and security status is rated at below the average. Documents regulating the life safety of pop ulation of NPP monitoring zone provide them no confidence in their security. Probability estimates of man made accidents are higher in urban population and depend on education level. The socialized health care is assessed on low and average levels according to the studied parameters. Among the types of medical care the providing of nec essary medical goods, preventive examinations, scheduled medical examination service, ambulance activity, and medical psychological aid need to be improved. There was no significant change in resource potential of special ized healthcare infirmary of NPP overspill town for the last 5 years. Low rating by the monitoring zone population of work efficiency of health facilities is determined by a set of factors, some of which lies in the plane of state socio economic problems. Choice priority of the direct sub ventions in population of monitoring zone depends on the place of

  1. Direct digital control of furnaces irradiated in nuclear reactors; Surveillance et regulation multiplexee par calculateur numerique de fours irradies

    Energy Technology Data Exchange (ETDEWEB)

    Joumard, R [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1969-07-01

    An experimental direct digital control system has been realised in the 'C.E.N.G.', in order to verify that a computer makes easier the control of the experiments done in the nuclear reactors and to solve the theoretical and technical difficulties. The regulation is applied to thermal processes. The sampled data systems theory permits to choose the type of an efficient and simple digital compensator, and to establish a diagram which gives the values of the correcting parameters (obtained by minimizing the difference between the output and the input when perturbations occur). The programme execute, in simultaneity, supervision and regulation. Complex possibilities of printing out measures and alarms existed. The computer works out an incremental correction which makes step motors to turn. These motors act on the heating organs. The theoretical values and answers have been confirmed. The accuracy was limited essentially by the input quantification (1/1000 th). The comfort of such a system has been noticeable. (author) [French] Une installation de controle numerique direct fut realisee a titre experimental au C.E.N.G pour verifier qu'un ordinateur rendait plus aisee l'exploitation des experiences faites en pile nucleaire et pour degager les difficultes theoriques et techniques. La regulation s'applique a des processus thermiques. La theorie des systemes echantillonnes a permis de choisir un type de correcteur numerique simple et efficace et d'etablir un abaque qui donne les valeurs des parametres correcteurs minimisant les ecarts enregistres entre la reponse et la consigne en presence de perturbations. Le programme effectuait simultanement de la surveillance et de la regulation. Une restitution complexe des informations et des alarmes sur machine a ecrire etait possible. Le calculateur elaborait une correction incrementielle qui faisait tourner des moteurs pas a pas, lesquels commandaient les organes de puissance de chauffage. Les valeurs et les reponses theoriques ont

  2. Direct digital control of furnaces irradiated in nuclear reactors; Surveillance et regulation multiplexee par calculateur numerique de fours irradies

    Energy Technology Data Exchange (ETDEWEB)

    Joumard, R. [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1969-07-01

    An experimental direct digital control system has been realised in the 'C.E.N.G.', in order to verify that a computer makes easier the control of the experiments done in the nuclear reactors and to solve the theoretical and technical difficulties. The regulation is applied to thermal processes. The sampled data systems theory permits to choose the type of an efficient and simple digital compensator, and to establish a diagram which gives the values of the correcting parameters (obtained by minimizing the difference between the output and the input when perturbations occur). The programme execute, in simultaneity, supervision and regulation. Complex possibilities of printing out measures and alarms existed. The computer works out an incremental correction which makes step motors to turn. These motors act on the heating organs. The theoretical values and answers have been confirmed. The accuracy was limited essentially by the input quantification (1/1000 th). The comfort of such a system has been noticeable. (author) [French] Une installation de controle numerique direct fut realisee a titre experimental au C.E.N.G pour verifier qu'un ordinateur rendait plus aisee l'exploitation des experiences faites en pile nucleaire et pour degager les difficultes theoriques et techniques. La regulation s'applique a des processus thermiques. La theorie des systemes echantillonnes a permis de choisir un type de correcteur numerique simple et efficace et d'etablir un abaque qui donne les valeurs des parametres correcteurs minimisant les ecarts enregistres entre la reponse et la consigne en presence de perturbations. Le programme effectuait simultanement de la surveillance et de la regulation. Une restitution complexe des informations et des alarmes sur machine a ecrire etait possible. Le calculateur elaborait une correction incrementielle qui faisait tourner des moteurs pas a pas, lesquels commandaient les organes de puissance de chauffage. Les valeurs

  3. A new method for improving the reliability of fracture toughness surveillance of nuclear pressure vessel by neutron irradiated embrittlement

    International Nuclear Information System (INIS)

    Zhang Xinping; Shi Yaowu

    1992-01-01

    In order to obtain more information from neutron irradiated sample specimens and raise the reliability of fracture toughness surveillance test, it has more important significance to repeatedly exploit the broken Charpy-size specimen which had been tested in surveillance test. In this work, on the renewing design and utilization for Charpy-size specimens, 9 data of fracture toughness can be gained from one pre-cracked side-grooved Charpy-size specimen while at the preset usually only 1 to 3 data of fracture toughness can be obtained from one Chharpy-size specimen. Thus, it is found that the new method would obviously improve the reliability of fracture toughness surveillance test and evaluation. Some factors which affect the reasonable design of pre-cracked deep side-groove Charpy-size compound specimen have been discussed

  4. Report on SUJB results achieved in the surveillance of nuclear facilities and radiation protection for 2005. Pt. 1

    International Nuclear Information System (INIS)

    2006-01-01

    This part of the report consists of the following chapters: (1) State Office for Nuclear Safety; (2) Nuclear safety; (3) Spent fuel, rad-waste management, decommissioning; (4) Transport of nuclear materials, physical protection; (5) Radiation protection; (6) Emergency preparedness; (7) Radiation Monitoring Network in the Czech Republic; (8) Non -proliferation, nuclear, biological and chemical weapons; (9) International cooperation; (10) Science and research; (11) Free access to information; (12) Activities of institutes controlled by the State Office for Nuclear Safety. It is concluded that the operation of all Czech nuclear facilities, including the Dukovany and Temelin nuclear power plants, was safe and reliable in 2005. (P.A.)

  5. Medical surveillance according to the resolution radiation protection nuclear-power-law in working with ionizing radiation

    International Nuclear Information System (INIS)

    1988-01-01

    The rules with regard to the medical surveillance of persons who, during the execution of their duties, may be exposed to certain amounts of ionizing radiation are treated. After an explanation of the general starting points of the policy with regard to radiation hygiene, two governing tools are reviewed: the set of licences and the radiation hygiene standards. 10 refs.; 1 table

  6. Disaster prevention surveillance system

    International Nuclear Information System (INIS)

    Nara, Satoru; Kamiya, Eisei

    2001-01-01

    Fuji Electric Co., Ltd. has supplied many management systems to nuclear reactor institution. 'The nuclear countermeasures-against-calamities special-measures' was enforced. A nuclear entrepreneur has devised the measure about expansion prevention and restoration of a calamity while it endeavors after prevention of generating of a nuclear calamity. Our company have supplied the 'disaster prevention surveillance system' to the Japan Atomic Energy Research Institute Tokai Research Establishment aiming at strengthening of the monitoring function at the time (after the accident) of the accident used as one of the above-mentioned measures. A 'disaster prevention surveillance system' can share the information on the accident spot in an on-site command place, an activity headquarters, and support organizations, when the serious accident happens. This system is composed of various sensors (temperature, pressure and radiation), cameras, computers and network. (author)

  7. Presence of albumin mRNA precursors in nuclei of analbuminemic rat liver lacking cytoplasmic albumin mRNA.

    OpenAIRE

    Esumi, H; Takahashi, Y; Sekiya, T; Sato, S; Nagase, S; Sugimura, T

    1982-01-01

    Analbuminemic rats, which lack serum albumin, were previously found to have no albumin mRNA in the cytoplasm of the liver. In the present study, the existence of nuclear albumin mRNA precursors in the liver of analbuminemic rats was examined by RNA X cDNA hybridization kinetics. Albumin mRNA precursors were present in the nuclei of analbuminemic rat liver at almost normal levels, despite the absence of albumin mRNA from the cytoplasm. Nuclear RNA of analbuminemic rat liver was subjected to el...

  8. Effect of PCB 126 on aryl hydrocarbon receptor 1 (AHR1) and AHR1 nuclear translocator 1 (ARNT1) mRNA expression and CYP1 monooxygenase activity in chicken (Gallus domesticus) ovarian follicles.

    Science.gov (United States)

    Wójcik, Dagmara; Antos, Piotr A; Katarzyńska, Dorota; Hrabia, Anna; Sechman, Andrzej

    2015-12-03

    The aim of the experiment was to study the in vitro effect of 3,3',4,4',5-pentachlorobiphenyl (PCB 126; a coplanar PCB congener) on aryl hydrocarbon receptor (AHR1) and AHR1 nuclear translocator (ARNT1) mRNA expression and the activity of CYP1 family monooxygenases in chicken ovarian follicles. White (1-4 mm) and yellowish (4-8 mm) prehierarchical follicles as well as fragments of the theca and granulosa layers of the 3 largest preovulatory follicles (F3-F1) were incubated in a medium supplemented with 0 (control group), 1, 10 or 100 nM PCB 126. The incubation was carried out for 6 h or 24 h for determination of mRNA expression of AHR1 and ARNT1 genes (real-time qPCR) and CYP1 monooxygenase activity (EROD and MROD fluorometric assays), respectively. It was found that chicken ovarian follicles express mRNA of AHR1 and ARNT1 genes. A modulatory effect of PCB 126 on AHR1 and ARNT1 expression depended not only on the biphenyl concentration but also on the follicular layer and the maturational state of the follicle. EROD and MROD activities appeared predominantly in the granulosa layer of the yellow preovulatory follicles. PCB 126 induced these activities in a dose-dependent manner in all ovarian follicles. The obtained results suggest that ovarian follicles, especially the granulosa layer, are involved in the detoxification process of PCBs in the laying hen. Taking this finding into consideration it can be suggested that the granulosa layer of the yellow hierarchical follicles plays a key role in the protective mechanism which reduces the amount of transferred dioxin-like compounds into the yolk of the oocyte. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  9. Surveillance of radioactivity in the atmosphere by the Deutscher Wetterdienst (DWD) in the framework of nuclear emergency response programmes

    International Nuclear Information System (INIS)

    Steinkopff, T.; Dalheimer, A.; Dyck, W.; Fay, B.; Glaab, H.; Jacobsen, I.

    2000-01-01

    The Deutscher Wetterdienst (DWD), German Meteorological Service, is charged with the surveillance of radioactivity in the atmosphere as a part of the emergency information network of the 'Integrated Measurement and Information System' (IMIS) in Germany. The results of measurements of radioactivity and the meteorological products are transferred regularly to this network. The DWD is also integrated into the Environmental Emergency Response Programme (EER) of the World Meteorological Organization (WMO) as a communication hub. The computer infrastructure, the operational experience in data management as well as the national and international communication systems in operation are significant arguments to run the early alert system on the surveillance of atmospheric radioactivity at the national meteorological service. (author)

  10. Development of a sealing process of capsules for surveillance test tubes of the vessel in nuclear power plants

    International Nuclear Information System (INIS)

    Romero C, J.; Fernandez T, F.; Perez R, N.; Rocamontes A, M.; Garcia R, R.

    2007-01-01

    The surveillance capsule is composed by the support, three capsules for impact test tubes, five capsules for tension test tubes and one porta dosemeters. The capsules for test tubes are of two types: rectangular capsule for Charpy test tubes and cylindrical capsule for tension test tubes. This work describes the development of the welding system to seal the capsules for test tubes that should contain helium of ultra high purity to a pressure of 1 atmosphere. (Author)

  11. Temporal regulation of HTLV-2 expression in infected cell lines and patients: evidence for distinct expression kinetics with nuclear accumulation of APH-2 mRNA

    Directory of Open Access Journals (Sweden)

    Bender Cecilia

    2012-09-01

    Full Text Available Abstract Background Human T-cell leukemia virus types 1 and 2 (HTLV-1 and HTLV-2 are delta retroviruses with similar genetic organization. Although both viruses immortalize T-cells in vitro, they exhibit distinct pathogenic potential in vivo. To search for possible differences in its expression strategy with respect to HTLV-1, we investigated the pattern of HTLV-2 expression in infected cell lines and peripheral blood mononuclear cells (PBMCs from infected patients using splice site-specific quantitative RT-PCR. Findings A novel alternative splice acceptor site for exon 2 was identified; its usage in env transcripts was found to be subtype-specific. Time-course analysis revealed a two-phase expression kinetics in an infected cell line and in PBMCs of two of the three patients examined; this pattern was reminiscent of HTLV-1. In addition, the minus-strand APH2 transcript was mainly detected in the nucleus, a feature that was similar to its HTLV-1 orthologue HBZ. In contrast to HTLV-1, expression of the mRNA encoding the main regulatory proteins Tax and Rex and that of the mRNAs encoding the p28 and truncated Rex inhibitors is skewed towards p28/truncated Rex inhibitors in HTLV-2. Conclusion Our data suggest a general converging pattern of expression of HTLV-2 and HTLV-1 and highlight peculiar differences in the expression of regulatory proteins that might influence the pathobiology of these viruses.

  12. Dietary Selenium Deficiency or Excess Reduces Sperm Quality and Testicular mRNA Abundance of Nuclear Glutathione Peroxidase 4 in Rats.

    Science.gov (United States)

    Zhou, Ji-Chang; Zheng, Shijie; Mo, Junluan; Liang, Xiongshun; Xu, Yuanfei; Zhang, Huimin; Gong, Chunmei; Liu, Xiao-Li; Lei, Xin Gen

    2017-10-01

    Background: Glutathione peroxidase (GPX) 4 and selenoprotein P (SELENOP) are abundant, and several variants are expressed in the testis. Objective: We determined the effects of dietary selenium deficiency or excess on sperm quality and expressions of GPX4 and SELENOP variants in rat testis and liver. Methods: After weaning, male Sprague-Dawley rats were fed a Se-deficient basal diet (BD) for 5 wk until they were 9 wk old [mean ± SEM body weight (BW) = 256 ± 5 g]. They were then fed the BD diet alone (deficient) or with 0.25 (adequate), 3 (excess), or 5 (excess) mg Se/kg for 4 wk. Testis, liver, blood, and semen were collected to assay for selenoprotein mRNA and protein abundances, selenium concentration, GPX activity, 8-hydroxy-deoxyguanosine concentration, and sperm quality. Results: Dietary selenium supplementations elevated ( P selenium concentrations and GPX activities. Compared with those fed BD + 0.25 mg Se/kg, rats fed BD showed lower ( P selenium-adequate group. Compared with the selenium-adequate group, dietary selenium deficiency (BD) or excess (BD + 3 or 5 mg Se/kg) resulted in 45-77% lower ( P selenium concentrations in similar ways to sperm parameters and may be used as a sensitive marker to assess appropriate Se status for male function. © 2017 American Society for Nutrition.

  13. Introduction to surveillance studies

    CERN Document Server

    Petersen, JK

    2012-01-01

    Introduction & OverviewIntroduction Brief History of Surveillance Technologies & TechniquesOptical SurveillanceAerial Surveillance Audio Surveillance Radio-Wave SurveillanceGlobal Positioning Systems Sensors Computers & the Internet Data Cards Biochemical Surveillance Animal Surveillance Biometrics Genetics Practical ConsiderationsPrevalence of Surveillance Effectiveness of Surveillance Freedom & Privacy IssuesConstitutional Freedoms Privacy Safeguards & Intrusions ResourcesReferences Glossary Index

  14. Final Report for the 1st Surveillance Test of the Reactor Pressure Vessel Material (Capsule 2) of Ulchin Nuclear Power Plant Unit 4

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Kee Ok; Kim, Byoung Chul; Lee, Sam Lai (and others)

    2007-04-15

    Surveillance testing for reactor vessel materials is performed in order to evaluate the irradiation embrittlement due to neutrons during operation and set the condition of safe operation of nuclear reactor. The 1st surveillance testing was performed completely by Korea Atomic Energy Research Institute at Daejon after the capsule was transported from Ulchin site including its removal from reactor. Fast neutron fluences for capsules were calculated and various testing including mechanical and chemistry analysis were performed in order to evaluate the integrity of Ulchin Unit 4 reactor vessel during the operation until life time. The evaluation results are as follows; Fast neutron fluences for capsule 2 is 4.306E+18n/cm{sup 2}. The bias factor, the ratio of calculation/measurement, was 0.918 for the 1st testing and the calculational uncertainty,7.0% satisfied the requirement of USNRC Reg.Guide 1.190, 20%. The best estimated neutron fluence for reactor vessel inside surface was 3.615E+18n/cm{sup 2} based on the end of 6th fuel cycle and it was predicted that the fluences of vessel inside surface at 16 and 32EFPY would reach 8.478E+18 and 1.673E+19n/cm{sup 2} based on the current calculation. The result through this analysis for Ulchin Unit 4 showed that there would be no problem for the pressurized thermal shock(PTS) during the operation until design life.

  15. Final Report for the 1st Surveillance Test of the Reactor Pressure Vessel Material (CAPSULE 2) of Ulchin Nuclear Power Plant Unit 3

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Kee Ok; Kim, Byoung Chul; Lee, Sam Lai (and others)

    2006-12-15

    Surveillance testing for reactor vessel materials is performed in order to evaluate the irradiation embrittlement due to neutrons during operation and set the condition of safe operation of nuclear reactor. The 1st surveillance testing was performed completely by Korea Atomic Energy Research Institute at Taejon after the capsule was transported from Ulchin site including its removal from reactor. Fast neutron fluences for capsules were calculated and various testing including mechanical and chemistry analysis were performed in order to evaluate the integrity of Ulchin unit 3 reactor vessel during the operation until life time. The evaluation results are as follows; Fast neutron fluences for capsule 2 is 4.674E 18n/cm{sup 2}. The bias factor, the ratio of calculation/measurement, was 0.920 for the 1st testing and the calculational uncertainty,7.0% satisfied the requirement of USNRC Reg.Guide 1.190, 20%. The best estimated neutron fluence for reactor vessel inside surface was 3.913E 18n/cm{sup 2} based on the end of 6th fuel cycle and it was predicted that the fluences of vessel inside surface at 16 and 32EFPY would reach 9.249E 18 and 1.834E 19n/cm{sup 2} based on the current calculation. The result through this analysis for Ulchin unit 3 showed that there would be no problem for the pressurized thermal shock(PTS) during the operation until design life.

  16. Visual interface for the automation of the instrumented pendulum of Charpy tests used in the surveillance program of reactors vessel of nuclear power plants

    International Nuclear Information System (INIS)

    Rojas S, A.S.; Sainz M, E.; Ruiz E, J.A.

    2004-01-01

    Inside the Programs of Surveillance of the nuclear power stations periodic information is required on the state that keep the materials with those that builds the vessel of the reactor. This information is obtained through some samples or test tubes that are introduced inside the core of the reactor and it is observed if its physical characteristics remain after having been subjected to the radiation changes and temperature. The rehearsal with the instrumented Charpy pendulum offers information on the behavior of fracture dynamics of a material. In the National Institute of Nuclear Research (ININ) it has an instrumented Charpy pendulum. The operation of this instrument is manual, having inconveniences to carry out rehearsals with radioactive material, handling of high and low temperatures, to fulfill the normative ones for the realization of the rehearsals, etc. In this work the development of a computational program is presented (virtual instrument), for the automation of the instrumented pendulum. The system has modules like: Card of data acquisition, signal processing, positioning system, tempered system, pneumatic system, compute programs like it is the visual interface for the operation of the instrumented Charpy pendulum and the acquisition of impact signals. This system shows that given the characteristics of the nuclear industry with radioactive environments, the virtual instrumentation and the automation of processes can contribute to diminish the risks to the personnel occupationally exposed. (Author)

  17. Epidemiological Surveillance at Electricite de France and Gaz de France: Health Assessment of Nuclear Power Plant Employers between 1993 and 1998

    Energy Technology Data Exchange (ETDEWEB)

    Chevalier, A.; Gros, H.; Carrier, E.; Labon, G.

    2004-07-01

    Because the 17,500 employees working in nuclear power plants at Electricite de France, the national power company, may be exposed to a wide variety of industrial hazards, the health insurance department of the company has set up an epidemiologic surveillance programme for them. This descriptive, cross-sectional,and exhaustive study examined sick-leaves, mortality and cancer incidence to assess the health of the employees working from 1993 through 1998. The analysis comparing the employees in nuclear power plants, considered exposed, to the rest of the personnel of Electricite de France-Gaz de France, the non-exposed (125,000 persons) showed that globally, the employees in the nuclear sector appeared to have fewer health problems than the other company employees. This was true regardless of age and especially for men, operating employees, and supervisory employees. Nonetheless three points must be noted: non-work accidents generated a non-significant excess of absenteeism and mortality among these employees, especially among management and supervisory personnel; suicides affected supervisors in particular; and an excess of primary malignant brain tumors affected both mortality (RR{sub 1}.96, NS) and incidence, especially among operating employees (RR=2.87, 95% CL=(1.00-8.43)). No excess of malignant blood disease was observed. (Author) 38 refs.

  18. Epidemiological Surveillance at Electricite de France and Gaz de France: Health Assessment of Nuclear Power Plant Employers between 1993 and 1998

    International Nuclear Information System (INIS)

    Chevalier, A.; Gros, H.; Carrier, E.; Labon, G.

    2004-01-01

    Because the 17,500 employees working in nuclear power plants at Electricite de France, the national power company, may be exposed to a wide variety of industrial hazards, the health insurance department of the company has set up an epidemiologic surveillance programme for them. This descriptive, cross-sectional,and exhaustive study examined sick-leaves, mortality and cancer incidence to assess the health of the employees working from 1993 through 1998. The analysis comparing the employees in nuclear power plants, considered exposed, to the rest of the personnel of Electricite de France-Gaz de France, the non-exposed (125,000 persons) showed that globally, the employees in the nuclear sector appeared to have fewer health problems than the other company employees. This was true regardless of age and especially for men, operating employees, and supervisory employees. Nonetheless three points must be noted: non-work accidents generated a non-significant excess of absenteeism and mortality among these employees, especially among management and supervisory personnel; suicides affected supervisors in particular; and an excess of primary malignant brain tumors affected both mortality (RR 1 .96, NS) and incidence, especially among operating employees (RR=2.87, 95% CL=(1.00-8.43)). No excess of malignant blood disease was observed. (Author) 38 refs

  19. Evolution of computer-based surveillance, control and man-machine communication systems in nuclear power stations

    International Nuclear Information System (INIS)

    Wilkinson, H.M.

    1980-01-01

    Reactor surveillance systems, man-machine communication, control methods and system relibility have steadily improved with successive designs. These improvements have been dramatic. Operational results from nine power-reactor units and preliminary results from 12 more units in various phases of implementation are now becoming available. The scope of the improvements ranges from the first design which used only lantern type displays to the latest which uses 18 colour cathode ray tubes (CRT). The operator can bnow scan a parallel presentation of annunciator messages, bar charts and graphical presentations. (orig./HP)

  20. Nanostructural evolution in surveillance test specimens of a commercial nuclear reactor pressure vessel studied by three-dimensional atom probe and positron annihilation

    International Nuclear Information System (INIS)

    Toyama, T.; Nagai, Y.; Tang, Z.; Hasegawa, M.; Almazouzi, A.; Walle, E. van; Gerard, R.

    2007-01-01

    The nanostructural evolution of irradiation-induced Cu-rich nanoprecipitates (CRNPs) and vacancy clusters in surveillance test specimens of in-service commercial nuclear reactor pressure vessel steel welds of Doel-1 and Doel-2 are revealed by combining the three-dimensional local electrode atom probe and positron annihilation techniques. In both medium (0.13 wt.%) and high (0.30 wt.%) Cu welds, the CRNPs are found to form readily at the very beginning of the reactor lifetime. Thereafter, during the subsequent 30 years of operation, the residual Cu concentration in the matrix shows a slight decrease while the CRNPs coarsen. On the other hand, small vacancy clusters of V 3 -V 4 start appearing after the initial Cu precipitation and accumulate steadily with increasing neutron dose. The observed nanostructural evolution is shown to provide unique and fundamental information about the mechanisms of the irradiation-induced embrittlement of these specific materials

  1. Surveillance Culture

    DEFF Research Database (Denmark)

    2017-01-01

    What does it mean to live in a world full of surveillance? In this documentary film, we take a look at everyday life in Denmark and how surveillance technologies and practices influence our norms and social behaviour. Researched and directed by Btihaj Ajana and Anders Albrechtslund....

  2. Optimization and studies of the welding processes, automation of the sealing welding system and fracture mechanics in the vessels surveillance in nuclear power plants

    International Nuclear Information System (INIS)

    Gama R, G.

    2011-01-01

    Inside this work the optimization of two welding systems is described, as well as the conclusion of a system for the qualification of containers sealing in the National Institute of Nuclear Research that have application in the surveillance programs of nuclear reactors vessels and the correspondent extension of the operation license. The test tubes Charpy are assay to evaluate the embrittlement grade, when obtaining the increment in the reference temperature and the decrease of the absorbed maximum energy, in the transition curve fragile-ductile of the material. After the test two test tube halves are obtained that should take advantage to follow the surveillance of the vessel and their possible operation extension, this is achieved by means of rebuilding (being obtained of a tested test tube two reconstituted test tubes). The welding system for the rebuilding of test tubes Charpy, was optimized when diminishing the union force at solder, achieving the elimination of the rejection for penetration lack for spill. For this work temperature measurements were carried out at different distances of the welding interface from 1 up to 12 mm, obtaining temperature profiles. With the maximum temperatures were obtained a graph and equation that represents the maximum temperature regarding the distance of the interface, giving as a result practical the elimination of other temperature measurements. The reconstituted test tubes were introduced inside pressurized containers with helium of ultra high purity to 1 pressure atmosphere. This process was carried out in the welding system for containers sealing, where an automatic process was implemented by means of an application developed in the program LabVIEW, reducing operation times and allowing the remote control of the process, the acquisition parameters as well as the generation of welding reports, avoiding with this the human error. (Author)

  3. Passive surveillance: a technique to characterize the condition of power and control circuits in a nuclear plant

    International Nuclear Information System (INIS)

    Meininger, R.D.; Dinsel, M.R.

    1985-01-01

    This paper reports on progress by EG and G Idaho in examination of electrical circuits exposed to the accident environment at Three Mile Island Unit 2 (TMI-2) during and after the loss-of-coolant accident of March 28, 1979. Interpretations of the data collected suggest that the major functional impact on the electrical circuits (a) occurs very late in time, (b) is caused by moisture intrusion, and (c) can be detected by remote surveillance prior to functional failure. The electrical testing was performed from outside the TMI-2 Reactor Building at the penetrations using a special circuit characterization and diagnostic system developed by EG and G Idaho. This paper concentrates on representative data from those circuits which were recently retested. 12 refs., 9 figs

  4. Bivalve fouling of nuclear power plant service-water systems. Volume 2. Current status of biofouling surveillance and control techniques

    International Nuclear Information System (INIS)

    Daling, P.M.; Johnson, K.I.

    1985-03-01

    This report describes the current status of techniques for detection and control of cooling-water system fouling by bivalve mollusks at nuclear power plants. The effectiveness of these techniques is evaluated on the basis of information gathered from a literature review and in interviews with nuclear power plant personnel. Biofouling detection techniques examined in this report include regular maintenance, in-service inspection, and testing. Generally, these methods have been inadequate for detecting biofouling. Recommendations for improving biofouling detection capabilities are presented. Biofouling prevention (or control) methods that are examined in this report include intake screen systems, thermal treatment, preventive maintenance, chemical treatment alternatives, and antifoulant coatings. Recommendations for improving biofouling control methods at operating nuclear power plants are presented. Additional techniques that could be implemented at future power plants or that require further research are also described

  5. Surveillance Pleasures

    DEFF Research Database (Denmark)

    Albrechtslund, Anders

    The notorious intensification and digitalization of surveillance technologies and practices in today’s society has brought about numerous changes. These changes have been widely noticed, described and discussed across many academic disciplines. However, the contexts of entertainment, play...

  6. Organisation and operation of radioactivity surveillance and control in the vicinity of nuclear plants. A practical guide

    International Nuclear Information System (INIS)

    1975-01-01

    Guidance is presented for the development of environmental monitoring programmes and procedures relevant to the operation of nuclear plants. The essential aim of an environmental monitoring programme is to ensure that the exposure of the general public does not exceed certain set limits. A certain uniformity of criteria in the organization of monitoring networks around nuclear installations may prove to be very useful. These criteria are set out in detail. Progress is reported on the following studies: monitoring of radioactive contamination of atmosphere, water, soils and foodstuffs: monitoring of external radiation

  7. Concepts for the calculation of radiation exposure in the environment of nuclear plants for planning and surveillance purposes

    International Nuclear Information System (INIS)

    Brenk, H.D.; Vogt, K.J.; Bruessermann, K.; Schwarz, G.

    1977-01-01

    In connection with the release of radioactive substances from nuclear plants, the following requirements are to be met in respect of the assessment of radiation exposure of persons in the environment of the plant: for the purpose of planning and licencing nuclear plants, the release rates of radioactive substances are to be limited to such a degree that the dose limit values specified in the Radiation Protection Ordinance are not exceeded at any time or on any site. This applies possibly under consideration of the pre-exposure rate. For long-lived radionuclides this requirement involves the calculation of annual doses at the end of a period determined by the time of operation of the plant and by the exposure time of the persons. During the operation of nuclear plants it is necessary to calculate the radiation exposure rates resulting from the emission measured for the year of reference. This application requires the calculation of the dose commitment resulting in the future on the basis of annual emissions for persons living in the environment of the plant. In connection with the long-term prediction of the environmental impact caused by the entire nuclear industry, problems will also be arising in conjunction with the case history of the environmental exposure being subject to respective alterations as a result of additional plants

  8. Final report for the 5th surveillance test of the reactor pressure vessel material (capsule Y) of Yonggwang Nuclear Power Plant unit 2

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Sam Lai; Kim, ByoungChul; Chang, Kee Ok (and others)

    2006-02-15

    Surveillance testing for reactor vessel materials is performed in order to evaluate the irradiation embrittlement due to neutrons during operation and set the condition of safe operation of nuclear reactor. The 5th surveillance testing was performed completely by Korea Atomic Energy Research Institute at Daejeon after the capsule was transported from Yonggwang site including its removal from reactor. Fast neutron fluences for capsules were calculated and various testing including mechanical and chemistry analysis were performed in order to evaluate the integrity of Yonggwang unit 2 reactor vessel during the operation until life time. The evaluation results are as follows; Fast neutron fluences for capsules U, V, X, W and Y are 5.777E+18, 1.5371E+19, 3.7634E+19, 4.3045E+19, and 4.8662E+19n/cm{sup 2}, respectively. The bias factor, the ratio of calculation/measurement, was 0.953 for the 1st through 5th testing and the calculational uncertainty,7.2% satisfied the requirement of USNRC Reg.Guide 1.190, 20%. The best estimated neutron fluence for reactor vessel inside surface was 1.659E+19n/cm{sup 2} based on the end of 13th fuel cycle and it was predicted that the fluences of vessel inside surface at 32, 48, 56 and 64EFPY would reach 3.625E+19, 5.293E+19, 6.127E+19 and 6.960E+19n/cm{sup 2} based on the current calculation. The result through this analysis for Yonggwang unit 2 showed that there would be no problem for the Pressurized Thermal Shock(PTS) during the operation until design life.

  9. The 4th surveillance test and evaluation of the reactor pressure vessel material (capsule W) of Younggwang nuclear power plant unit1

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Byoung Chul; Lee, Sam Lai; Choi, Kwon Jae [Korea Atomic Energy Research Institute, Taejeon (Korea)

    2001-08-01

    Surveillance testing for reactor vessel materials is performed in order to evaluate the irradiation embrittlement due to neutrons during operation and set the condition of safe operation of nuclear reactor. The 4th surveillance testing was performed completely by Korea Atomic Energy Research Institute at Taejeon after the capsule was transported from Yonggwang site including its removal from reactor. Fast neutron fluences for capsules were calculated and various testing including mechanical and chemistry analysis were performed in order to evaluate the integrity of Yonggwang unit 1 reactor vessel during the operation until life time. The evaluation results are as follows; Fast neutron fluences for capsules U, V, X and W are 5.555E+18, 1.662E+19, 3.358E+19, and 4.521E+19 n/cm{sup 2}, respectively. The bias factor, the ratio of measurement versus calculation, was 0.859 for the 1st through 4th testing and the calculational uncertainty, 11.80% satisfied the requirement of USNRC Reg.Guide 1.190, 20%. The best estimated neutron fluence for reactor vessel inside surface was 1.551E+19 n/cm{sup 2} based on the end of 12th fuel cycle and it was predicted that the fluences of vessel inside surface at 32, 40, 48 and 56EFPY would reach 3.929E+19, 4.880E+19, 5.831E+19 and 6.782E+19 n/cm{sup 2} based on the current calculation. The result through this analysis for Yonggwang unit 1 showed that there would be no problem for the pressurized thermal shock(PTS) during the operation until design life. 4 refs., 41 figs., 35 tabs. (Author)

  10. The 4th surveillance test and evaluation of the reactor pressure vessel material (capsule W) of Yonggwang nuclear power plant unit 2

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Kee Ok; Kim, Byoung Chul; Lee, Sam Lai; Choi, Kwon Jae; Gong, Un Sik; Chang, Jong Hwa; Joo, Yong Sun; Ahn, Sang Bok; Hong, Joon Hwa [Korea Atomic Energy Research Institute, Taejeon (Korea)

    2001-02-01

    Surveillance testing for reactor vessel materials is performed in order to evaluate the irradiation embrittlement due to neutrons during operation and set the condition of safe operation of nuclear reactor. The 4th surveillance testing was performed completely by Korea Atomic Energy Research Institute at Taejeon after the capsule was transported from Kori site including its removal from reactor. Fast neutron fluences for capsules were calculated and various testing including mechanical and chemistry analysis were performed in order to evaluate the integrity of Yonggwang unit 2 reactor vessel during the operation until life time. The evaluation results are as follows; Fast neutron fluences for capsules U, V, X and W are 5.762E+18, 1.5391E+19, 3.5119E+19, and 4.2610E+19 n/cm{sup 2}, respectively. The bias factor, the ratio of measurement versus calculation, was 0.899 for the 1st through 4th testing and the calculational uncertainty, 12.3% satisfied the requirement of USNRC Reg.Guide DG-1053, 20%. The best estimated neutron fluence for reactor vessel inside surface was 1.357E+19 n/cm{sup 2} based on the end of 11th fuel cycle and it was predicted that the fluences of vessel inside surface at 32, 40, 48 and 56EFPY would reach 3.525E+19, 4.337E+19, 5.148E+19 and 5.960E+19 n/cm{sup 2} based on the current calculation. The result through this analysis for Yonggwang unit 2 showed that there would be no problem for the pressurized thermal shock(PTS) during the operation until design life. 48 refs., 35 figs., 42 tabs. (Author)

  11. Cloning of the DNA-binding subunit of human nuclear factor κB: The level of its mRNA is strongly regulated by phorbol ester or tumor necrosis factor α

    International Nuclear Information System (INIS)

    Meyer, R.; Hatada, E.N.; Bartsch, C.; Scheidereit, C.; Hohmann, H.P.; Haiker, M.; Roethlisberger, U.; Lahm, H.W.; Schlaeger, E.J.; van Loon, A.P.G.M.

    1991-01-01

    The DNA binding subunit of nuclear factor κB (NF-κB), a B-cell protein that interacts with the immunoglobulin κ light-chain gene enhancer, has been purified from nuclei of human HL-60 cells stimulated with tumor necrosis factor α (TNFα), and internal peptide sequences were obtained. Overlapping cDNA clones were isolated and sequenced. The encoded open reading frame of about 105 kDa contained at its N-terminal half all six tryptic peptide sequences, suggesting that the 51-kDa NF-κB protein is processed from a 105-kDa precursor. An in vitro synthesized protein containing most of the N-terminal half of the open reading frame bound specifically to an NF-κB binding site. This region also showed high homology to a domain shared by the Drosophila dorsal gene and the avian and mammalian rel (proto)oncogene products. The level of the 3.8-kilobase mRNA was strongly increased after stimulation with TNFα or phorbol ester. Thus, both factors not only activate NF-κB protein, as described previously, but also induce expression of the gene encoding the DNA-binding subunit of NF-κB

  12. Cumulus expansion, nuclear maturation and connexin 43, cyclooxygenase-2 and FSH receptor mRNA expression in equine cumulus-oocyte complexes cultured in vitro in the presence of FSH and precursors for hyaluronic acid synthesis

    Directory of Open Access Journals (Sweden)

    Aiudi Giulio

    2004-06-01

    Full Text Available Abstract The aim of this study was to investigate cumulus expansion, nuclear maturation and expression of connexin 43, cyclooxygenase-2 and FSH receptor transcripts in equine cumuli oophori during in vivo and in vitro maturation in the presence of equine FSH (eFSH and precursors for hyaluronic acid synthesis. Equine cumulus-oocyte complexes (COC were cultured in a control defined medium supplemented with eFSH (0 to 5 micrograms/ml, Fetal Calf Serum (FCS, precursors for hyaluronic acid synthesis or glutamine according to the experiments. After in vitro maturation, the cumulus expansion rate was increased with 1 microgram/ml eFSH, and was the highest with 20% FCS. It was not influenced by precursors for hyaluronic acid synthesis or glutamine. The expression of transcripts related to cumulus expansion was analyzed in equine cumulus cells before maturation, and after in vivo and in vitro maturation, by using reverse transcription-polymerase chain reaction (RT-PCR with specific primers. Connexin 43, cyclooxygenase-2 (COX-2 and FSH receptor (FSHr mRNA were detected in equine cumulus cells before and after maturation. Their level did not vary during in vivo or in vitro maturation and was influenced neither by FSH nor by precursors for hyaluronic acid synthesis. Results indicate that previously reported regulation of connexin 43 and COX-2 proteins during equine COC maturation may involve post-transcriptional mechanisms.

  13. [Nuclear factor-kappaB mRNA and protein expression in stomach tissue of rats with gastric ulcer recurrence and effect of jianwei yuyang granule on its expression].

    Science.gov (United States)

    Ling, Jiang-Hong; Li, Jia-Bang; Shen, Ding-Zhu; Zhou, Bing

    2006-03-01

    To observe the inflammatory reaction, nuclear factor-kappaB (NF-kappaB) mRNA and protein expression in stomach tissue of rats with gastric ulcer recurrence and the effect of Jianwei Yuyang granule (JYG) on them. Gastric ulcer and its recurrent lesion were successively induced by acetic acid and interliukin1-beta (IL-1beta), and the model rats were divided into the sham operation group, the model group, the omeprazole (correction of omepraxole) group and the JYG group to observe the state of chronic inflammatory cell, neutrophil count, NF-kappaBmRNA and protein expression in stomach tissue. On the 16th and 92th day after administration, the increase of chronic inflammatory cell, neutrophil, NF-kappaBmRNA and protein expression in the model group was more significant than those in the sham operated group (P ulcer induced by acetic acid. JYG may suppress inflammatory reaction by inhibiting the activation and expression of NF-kappaB in stomach tissue, which may be one of the mechanisms of JYG in preventing the recurrence of gastric ulcer.

  14. Use of bioindicators in the environmental radiological surveillance of Mexican Nuclear Center; Utilizacion de biondicadores en la vigilancia radiologica ambiental del Centro Nuclear de Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Gaso P, M.I.; Segovia, N.; Herrera, T.; Perez S, E.; Cervantes, M.L.; Quintero, E.; Palacios, J.; Acosta, E. [Instituto Nacional de Investigaciones Nucleares, A.P. 18-1027, 11801 Mexico D.F. (Mexico); Herrera, T. [UNAM Instituto Biologia, 04510 Ciudad Univ., Mexico D.F. (Mexico)

    1998-07-01

    Cs-137 and K-40 concentrations were determined in soil samples and wild eatable mushrooms at the forest ecosystem of Mexican Nuclear Center and its surrounding area. The evaluation was carried out with a low bottom gamma spectroscopy system including a high-purity Ge detector (HPGe). The local species that showed greater soil-mushroom transference factor, for Cs-137, were Clavariadelphus truncatus, Cortinarius caerulescenc, Gomphus floccosus and Lyophyllum decastes. The Cs-137 levels obtained in the mushroom samples of Nuclear Center, were in general slightly lower than those of adjacent places, which indicates that this Center is not polluting the atmosphere with such radionuclide. (Author)

  15. Accountability and non-proliferation nuclear regime: a review of the mutual surveillance Brazilian-Argentine model for nuclear safeguards; Accountability e regime de nao proliferacao nuclear: uma avaliacao do modelo de vigilancia mutua brasileiro-argentina de salvaguardas nucleares

    Energy Technology Data Exchange (ETDEWEB)

    Xavier, Roberto Salles

    2014-08-01

    The regimes of accountability, the organizations of global governance and institutional arrangements of global governance of nuclear non-proliferation and of Mutual Vigilance Brazilian-Argentine of Nuclear Safeguards are the subject of research. The starting point is the importance of the institutional model of global governance for the effective control of non-proliferation of nuclear weapons. In this context, the research investigates how to structure the current arrangements of the international nuclear non-proliferation and what is the performance of model Mutual Vigilance Brazilian-Argentine of Nuclear Safeguards in relation to accountability regimes of global governance. For that, was searched the current literature of three theoretical dimensions: accountability, global governance and global governance organizations. In relation to the research method was used the case study and the treatment technique of data the analysis of content. The results allowed: to establish an evaluation model based on accountability mechanisms; to assess how behaves the model Mutual Vigilance Brazilian-Argentine Nuclear Safeguards front of the proposed accountability regime; and to measure the degree to which regional arrangements that work with systems of global governance can strengthen these international systems. (author)

  16. Surveillance of a nuclear reactor by pattern recognition analysis of the neutronic noise. Experience on Phenix LMFBR

    International Nuclear Information System (INIS)

    Le Guillou, G.; Bernardin, B.

    1980-05-01

    The purpose of this paper is to present some results of pattern recognition methods applied to the problem of supervising the behaviour of a nuclear reactor, especially the sodium cooled fast breeder Phenix. The problem to solve can be divided into two parts: first, from a set of data about the behaviour of the reactor, we have to find consistent classes of functionning. These classes must be interpreted by physical considerations; then, we have to elaborate a simple classification algorithm, which can be used in-line, to improve safety systems of fast breeder reactors, by giving helpful information for decision making. The neutronic noise has been chosen for our study

  17. Instrumentation of a Charpy-pendulum. Additional data obtained from it and its application to nuclear reactor pressure vessels surveillance programs

    International Nuclear Information System (INIS)

    Chomik, Enrique P.; Dhers, Horacio; Iorio, Antonio F.; Ciriani, Dario F.

    1999-01-01

    Charpy test gives information about a material dynamic fracture behavior. In a plain Charpy test, this information is the absorbed energy during fracture of the specimen, lateral deformation and the percentage of ductile fracture of the specimen. These parameters can then be used for the determination of the material response to a dynamic applied load, and are used at present to determine the brittle-ductile transition temperature of a material. However, there is a lot of additional information that can be obtained from a Charpy test, which is vital for the case of surveillance programs of nuclear power plants, where it is necessary to get the most available information from the specimens to be tested, because each one of them was irradiated for many years under temperature and neutronic flux conditions similar to that of the internal surface of the reactor pressure vessel, which converts these specimens in unique and very expensive ones. This additional information can be obtained from the curve that determines the evolution of the applied force to the specimen throughout the time involved in its fracture. It was possible to instrument a Charpy pendulum at a fraction of the cost necessary to buy an instrumentation package like the ones available in the market, and since the instrumentation equipment obtained is easy to transport. It has the additional advantage that can be used to instrument any other pendulum replacing only the hammer of the pendulum with a instrumented one for that pendulum. (author)

  18. Social and ecological factors in the formation of population health in surveillance zone of nuclear power plants

    International Nuclear Information System (INIS)

    Prilipko, V.A.; Petrichenko, O.O.

    2014-01-01

    Most people of radiation-control area estimated ecological state as safe. Respondents pointed to such ecological factors as radionuclide pollutions, irresponsible ecological behavior of people, Rivne Nuclear Power Plant functioning consequences. The last one affects changes of water sanitation and ecological parameters.There is no significant difference between mental state of people of radiation-control area and control group of people by GHQ-28 indices, by somatization scale, anxiety, social disfunction and reactive anxiety. The living life peculiarities are grounded by double risk: life in conditions of radioactive polluted territory and life in radiation-control area. Authors defined the correlation between mental state and factors that characterize ecological living conditions

  19. Influenza surveillance

    Directory of Open Access Journals (Sweden)

    Karolina Bednarska

    2016-04-01

    Full Text Available Influenza surveillance was established in 1947. From this moment WHO (World Health Organization has been coordinating international cooperation, with a goal of monitoring influenza virus activity, effective diagnostic of the circulating viruses and informing society about epidemics or pandemics, as well as about emergence of new subtypes of influenza virus type A. Influenza surveillance is an important task, because it enables people to prepare themselves for battle with the virus that is constantly mutating, what leads to circulation of new and often more virulent strains of influenza in human population. As vaccination is the most effective method of fighting the virus, one of the major tasks of GISRS is developing an optimal antigenic composition of the vaccine for the current epidemic season. European Influenza Surveillance Network (EISN has also developed over the years. EISN is running integrated epidemiological and virological influenza surveillance, to provide appropriate data to public health experts in member countries, to enable them undertaking relevant activities based on the current information about influenza activity. In close cooperation with GISRS and EISN are National Influenza Centres - national institutions designated by the Ministry of Health in each country.

  20. Surveillance Angels

    NARCIS (Netherlands)

    Rothkrantz, L.J.M.

    2014-01-01

    The use of sensor networks has been proposed for military surveillance and environmental monitoring applications. Those systems are composed of a heterogeneous set of sensors to observe the environment. In centralised systems the observed data will be conveyed to the control room to process the

  1. Development of containers sealing system like part of surveillance program of the vessel in nuclear power plants; Desarrollo del sistema de sellado de contenedores como parte del programa de vigilancia de la vasija en nucleoelectricas

    Energy Technology Data Exchange (ETDEWEB)

    Romero C, J.; Hernandez C, R.; Fernandez T, F.; Rocamontes A, M.; Perez R, N. [ININ, Carretera Mexico-Toluca s/n, 52750 Ocoyoacac, Estado de Mexico (Mexico)], e-mail: jesus.romero@inin.gob.mx

    2009-10-15

    The owners of nuclear power plants should be demonstrate that the embrittlement effects by neutronic radiation do not commit the structural integrity from the pressure vessel of nuclear reactors, during conditions of routine operation and below postulate accident. For this reason, there are surveillance programs of vessels of nuclear power plants, in which are present surveillance capsules. A surveillance capsule is compound by the support, six containers for test tubes and dosimeters. The containers for test tubes are of two types: rectangular container for test tubes, Charpy V and Cylindrical Container for tension test tubes. These test tubes are subject to a same or bigger neutronic flow to that of vessel, being representative of vessel mechanical conditions. The test tubes are rehearsed to watch over the increase of embrittlement that presents the vessel. This work describes the development of welding system to seal the containers for test tubes, these should be filled with helium of ultra high purity, to a pressure of an atmosphere. In this system the welding process Gas Tungsten Arc Welding is used, a hermetic camera that allows to place the containers with three grades of freedom, a vacuum subsystem and pressure, high technology equipment's like: power source with integrated computer, arc starter of high frequency, helium flow controller, among others. Finally, the advances in the inspection system for the qualification of sealing system are mentioned, system that should measure the internal pressure of containers and the helium purity inside these. (Author)

  2. Surveillance of the environmental radioactivity

    International Nuclear Information System (INIS)

    Schneider, Th.; Gitzinger, C.; Jaunet, P.; Eberbach, F.; Clavel, B.; Hemidy, P.Y.; Perrier, G.; Kiper, Ch.; Peres, J.M.; Josset, M.; Calvez, M.; Leclerc, M.; Leclerc, E.; Aubert, C.; Levelut, M.N.; Debayle, Ch.; Mayer, St.; Renaud, Ph.; Leprieur, F.; Petitfrere, M.; Catelinois, O.; Monfort, M.; Baron, Y.; Target, A.

    2008-01-01

    The objective of these days was to present the organisation of the surveillance of the environmental radioactivity and to allow an experience sharing and a dialog on this subject between the different actors of the radiation protection in france. The different presentations were as follow: evolution and stakes of the surveillance of radioactivity in environment; the part of the European commission, regulatory aspects; the implementation of the surveillance: the case of Germany; Strategy and logic of environmental surveillance around the EDF national centers of energy production; environmental surveillance: F.B.F.C. site of Romans on Isere; steps of the implementation 'analysis for release decree at the F.B.F.C./C.E.R.C.A. laboratory of Romans; I.R.S.N. and the environmental surveillance: situation and perspectives; the part of a non institutional actor, the citizenship surveillance done by A.C.R.O.; harmonization of sampling methods: the results of inter operators G.T. sampling; sustainable observatory of environment: data traceability and samples conservation; inter laboratories tests of radioactivity measurements; national network of environmental radioactivity measurement: laboratories agreements; the networks of environmental radioactivity telemetry: modernization positioning; programme of observation and surveillance of surface environment and installations of the H.A.-M.A.V.L. project (high activity and long life medium activity); Evolution of radionuclides concentration in environment and adaptation of measurements techniques to the surveillance needs; the national network of radioactivity measurement in environment; modes of data restoration of surveillance: the results of the Loire environment pilot action; method of sanitary impacts estimation in the area of ionizing radiations; the radiological impact of atmospheric nuclear tests in French Polynesia; validation of models by the measure; network of measurement and alert management of the atmospheric

  3. SAVY-4000 Field Surveillance Plan Update for 2017

    Energy Technology Data Exchange (ETDEWEB)

    Kelly, Elizabeth J. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Stone, Timothy Amos [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Smith, Paul Herrick [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Reeves, Kirk Patrick [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Veirs, Douglas Kirk [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Prochnow, David Adrian [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2017-03-20

    The Packaging Surveillance Program section of the Department of Energy (DOE) Manual 441.1-­1, Nuclear Material Packaging Manual (DOE 2008), requires DOE contractors to “ensure that a surveillance program is established and implemented to ensure the nuclear material storage package continues to meet its design criteria.”This 2017 update reflects changes to the surveillance plan resulting from surveillance findings as documented in Reeves et al. 2016. These findings include observations of corrosion in SAVY and Hagan containers and the indication (in one SAVY container) of possible filter membrane thermal degradation. This surveillance plan update documents the rationale for selecting surveillance containers, specifies the containers for 2017 surveillance, and identifies a minimum set of containers for 2018 surveillance. This update contains important changes to the previous surveillance plans.

  4. Air surveillance

    International Nuclear Information System (INIS)

    Patton, G.W.

    1995-01-01

    This section of the 1994 Hanford Site Environmental Report summarizes the air surveillance and monitoring programs currently in operation at that Hanford Site. Atmospheric releases of pollutants from Hanford to the surrounding region are a potential source of human exposure. For that reason, both radioactive and nonradioactive materials in air are monitored at a number of locations. The influence of Hanford emissions on local radionuclide concentrations was evaluated by comparing concentrations measured at distant locations within the region to concentrations measured at the Site perimeter. This section discusses sample collection, analytical methods, and the results of the Hanford air surveillance program. A complete listing of all analytical results summarized in this section is reported separately by Bisping (1995)

  5. Air surveillance

    Energy Technology Data Exchange (ETDEWEB)

    Patton, G.W.

    1995-06-01

    This section of the 1994 Hanford Site Environmental Report summarizes the air surveillance and monitoring programs currently in operation at that Hanford Site. Atmospheric releases of pollutants from Hanford to the surrounding region are a potential source of human exposure. For that reason, both radioactive and nonradioactive materials in air are monitored at a number of locations. The influence of Hanford emissions on local radionuclide concentrations was evaluated by comparing concentrations measured at distant locations within the region to concentrations measured at the Site perimeter. This section discusses sample collection, analytical methods, and the results of the Hanford air surveillance program. A complete listing of all analytical results summarized in this section is reported separately by Bisping (1995).

  6. The particle swarm optimization algorithm applied to nuclear systems surveillance test planning; Otimizacao aplicada ao planejamento de politicas de testes em sistemas nucleares por enxame de particulas

    Energy Technology Data Exchange (ETDEWEB)

    Siqueira, Newton Norat

    2006-12-15

    This work shows a new approach to solve availability maximization problems in electromechanical systems, under periodic preventive scheduled tests. This approach uses a new Optimization tool called PSO developed by Kennedy and Eberhart (2001), Particle Swarm Optimization, integrated with probabilistic safety analysis model. Two maintenance optimization problems are solved by the proposed technique, the first one is a hypothetical electromechanical configuration and the second one is a real case from a nuclear power plant (Emergency Diesel Generators). For both problem PSO is compared to a genetic algorithm (GA). In the experiments made, PSO was able to obtain results comparable or even slightly better than those obtained b GA. Therefore, the PSO algorithm is simpler and its convergence is faster, indicating that PSO is a good alternative for solving such kind of problems. (author)

  7. Rinderpest surveillance

    International Nuclear Information System (INIS)

    2003-01-01

    Rinderpest is probably the most lethal virus disease of cattle and buffalo and can destroy whole populations; damaging economies; undermining food security and ruining the livelihood of farmers and pastoralists. The disease can be eradicated by vaccination and control of livestock movement. The Department of Technical Co-operation is sponsoring a programme, with technical support from the Joint FAO/IAEA Division to provide advice, training and materials to thirteen states through the 'Support for Rinderpest Surveillance in West Asia' project. (IAEA)

  8. Health surveillance

    International Nuclear Information System (INIS)

    1981-01-01

    The Code includes a number of requirements for the health surveillance of employees associated with the mining and milling of radioactive ores. This guideline is particularly directed at determining the level of fitness of employees and prospective employees, detecting any symptom which might contraindicate exposure to the environment encountered in mine/mill situations, examination of any employee who may have been exposed to radiation in excess of defined limits and the accumulation and provision of data on the health of employees

  9. Post-tensioning system surveillance program

    International Nuclear Information System (INIS)

    Drew, G.E.

    1979-01-01

    Nuclear power plant containment structure post-tensioning system tendon surveillance program is described in detail. Data collected over three yearly post-tensioning system Surveillance Programs is presented and evaluated to correlate anticipated stress losses with actual losses. In addition corrosion protected system performance is analyzed

  10. Medical surveillance of occupationally exposed workers

    International Nuclear Information System (INIS)

    2007-05-01

    The guide covers medical surveillance of workers engaged in radiation work and their fitness for this work, protection of the foetus and infant during the worker's pregnancy or breastfeeding, and medical surveillance measures to be taken when the dose limit has been exceeded. The guide also covers recognition of practitioners responsible for medical surveillance of category A workers, medical certificates to be issued to workers, and preservation and transfer of medical records. The medical surveillance requirements specified in this Guide cover the use of radiation and nuclear energy. The guide also applies to exposure to natural radiation in accordance with section 28 of the Finnish Radiation Decree

  11. Medical surveillance of occupationally exposed workers

    Energy Technology Data Exchange (ETDEWEB)

    2007-05-15

    The guide covers medical surveillance of workers engaged in radiation work and their fitness for this work, protection of the foetus and infant during the worker's pregnancy or breastfeeding, and medical surveillance measures to be taken when the dose limit has been exceeded. The guide also covers recognition of practitioners responsible for medical surveillance of category A workers, medical certificates to be issued to workers, and preservation and transfer of medical records. The medical surveillance requirements specified in this Guide cover the use of radiation and nuclear energy. The guide also applies to exposure to natural radiation in accordance with section 28 of the Finnish Radiation Decree

  12. Nutritional surveillance.

    Science.gov (United States)

    Mason, J B; Mitchell, J T

    1983-01-01

    The concept of nutritional surveillance is derived from disease surveillance, and means "to watch over nutrition, in order to make decisions that lead to improvements in nutrition in populations". Three distinct objectives have been defined for surveillance systems, primarily in relation to problems of malnutrition in developing countries: to aid long-term planning in health and development; to provide input for programme management and evaluation; and to give timely warning of the need for intervention to prevent critical deteriorations in food consumption. Decisions affecting nutrition are made at various administrative levels, and the uses of different types of nutritional surveillance information can be related to national policies, development programmes, public health and nutrition programmes, and timely warning and intervention programmes. The information should answer specific questions, for example concerning the nutritional status and trends of particular population groups.Defining the uses and users of the information is the first essential step in designing a system; this is illustrated with reference to agricultural and rural development planning, the health sector, and nutrition and social welfare programmes. The most usual data outputs are nutritional outcome indicators (e.g., prevalence of malnutrition among preschool children), disaggregated by descriptive or classifying variables, of which the commonest is simply administrative area. Often, additional "status" indicators, such as quality of housing or water supply, are presented at the same time. On the other hand, timely warning requires earlier indicators of the possibility of nutritional deterioration, and agricultural indicators are often the most appropriate.DATA COME FROM TWO MAIN TYPES OF SOURCE: administrative (e.g., clinics and schools) and household sample surveys. Each source has its own advantages and disadvantages: for example, administrative data often already exist, and can be

  13. mRNA processing in yeast

    International Nuclear Information System (INIS)

    Stevens, A.

    1982-01-01

    Investigations in this laboratory center on basic enzymatic reactions of RNA. Still undefined are reactions involved in the conversion of precursors of mRA (pre-mRNA) to mRNA in eukaryotes. The pre-mRNA is called heterogeneous nuclear RNA and is 2 to 6 times larger than mRNA. The conversion, called splicing, involves a removal of internal sequences called introns by endoribonuclease action followed by a rejoining of the 3'- and 5'-end fragments, called exons, by ligating activity. It has not been possible yet to study the enzymes involved in vitro. Also undefined are reactions involved in the turnover or discarding of certain of the pre-mRNA molecules. Yeast is a simple eukaryote and may be expected to have the same, but perhaps simpler, processing reactions as the higher eukaryotes. Two enzymes involved in the processing of pre-mRNA and mRNA in yeast are under investigation. Both enzymes have been partially purified from ribonucleoprotein particles of yeast. The first is a unique decapping enzyme which cleaves [ 3 H]m 7 Gppp [ 14 C]RNA-poly (A) of yeast, yielding [ 3 H]m 7 GDP and is suggested by the finding that the diphosphate product, m 7 GpppA(G), and UDP-glucose are not hydrolyzed. The second enzyme is an endoribonuclease which converts both the [ 3 H] and [ 14 C] labels of [ 3 H]m 7 Gppp[ 14 C]RNA-poly(A) from an oligo(dT)-cellulose bound form to an unbound, acid-insoluble form. Results show that the stimulation involves an interaction of the labeled RNA with the small nuclear RNA. The inhibition of the enzyme by ethidium bromide and its stimulation by small nuclear RNA suggest that it may be a processing ribonuclease, requiring specific double-stranded features in its substrate. The characterization of the unique decapping enzyme and endoribonuclease may help to understand reactions involved in the processing of pre-mRNA and mRNA in eukaryotes

  14. Surveillance and Critical Theory

    Directory of Open Access Journals (Sweden)

    Christian Fuchs

    2015-09-01

    Full Text Available In this comment, the author reflects on surveillance from a critical theory approach, his involvement in surveillance research and projects, and the status of the study of surveillance. The comment ascertains a lack of critical thinking about surveillance, questions the existence of something called “surveillance studies” as opposed to a critical theory of society, and reflects on issues such as Edward Snowden’s revelations, and Foucault and Marx in the context of surveillance.

  15. Neutron doze distribution in capsules for surveillance of radiation embrittlement of pressure vessel in Krsko nuclear power plant; Porazdelitev nevtronske doze v sondah za kontrolo povecanja krhkosri tlacne posode JE Krsko

    Energy Technology Data Exchange (ETDEWEB)

    Najzer, M; Remec, I; Kodeli, I [Institut Jozef Stefan, Ljubljana (Yugoslavia)

    1984-07-01

    Calculation of neutron fluence and spectrum distribution in the capsule with samples for radiation embrittlement of PWR pressure vessel surveillance program of Krsko nuclear power plant is presented. Two dimensional computer code DOT 3 has been used and neutron cross sections were taken from DLC-2D library. result is that fluence magnitude in the capsules changes for up to 70%, so when evaluating results of mechanical tests of samples it is necessary to take into account actual position of samples within the capsule. (author)

  16. Technical-evaluation report on the proposed technical-specification changes for the inservice surveillance of safety-related hydraulic and mechanical snubbers at the Millstone Nuclear Power Station, Unit 2 (Docket No. 50-336)

    International Nuclear Information System (INIS)

    Selan, J.C.

    1983-01-01

    This report documents the technical evaluation of the proposed Technical Specification changes to Limiting Conditions for Operation, Surveillance Requirements and Bases for safety-related hydraulic and mechanical snubbers at the Millstone Nuclear Power Station, Unit 2. The evaluation is to determine whether the proposed Technical Specifications are in conformance with the model Standard Technical Specification set forth by the NRC. A check list, Appendix A of this report, compares the licensee's submittal with the NRC requirements and includes Proposed Resolution of the Deviations

  17. Technical-evaluation report on the proposed technical-specification changes for the inservice surveillance of safety-related hydraulic and mechanical snubbers at the Indian Point Nuclear Power Plant, Unit 3 (Docket No. 50-286)

    International Nuclear Information System (INIS)

    Selan, J.C.

    1983-01-01

    This report documents the technical evaluation of the proposed Technical Specification changes to Limiting Conditions for Operation, Surveillance Requirements and Bases for safety-related hydraulic and mechanical snubbers at the Indian Point Nuclear Power Plant, Unit 3. The evaluation is to determine whether the proposed Technical Specifications are in conformance with the model Standard Technical Specification set forth by the NRC. A check list, Appendix A of this report, compares the licensee's submittal with the NRC requirements and includes Proposed Resolution of the Deviations

  18. Technical-evaluation report on the proposed technical-specification changes for the inservice surveillance of safety-related hydraulic and mechanical snubbers at the James A. Fitzpatrick Nuclear Power Plant (Docket No. 50-333)

    International Nuclear Information System (INIS)

    Selan, J.C.

    1983-01-01

    This report documents the technical evaluation of the proposed Technical Specification changes to Limiting Conditions for Operation, Surveillance Requirements and Bases for safety-related hydraulic and mechanical snubbers at the James A. FitzPatrick Nuclear Power Plant. The evaluation is to determine whether the proposed Technical Specifications are in conformance with the model Standard Technical Specification set forth by the NRC. A check list, Appendix A of this report, compares the licensee's submittal with the NRC requirements and includes Proposed Resolution of the Deviations

  19. A Methodology for Modeling Nuclear Power Plant Passive Component Aging in Probabilistic Risk Assessment under the Impact of Operating Conditions, Surveillance and Maintenance Activities

    Science.gov (United States)

    Guler Yigitoglu, Askin

    In the context of long operation of nuclear power plants (NPPs) (i.e., 60-80 years, and beyond), investigation of the aging of passive systems, structures and components (SSCs) is important to assess safety margins and to decide on reactor life extension as indicated within the U.S. Department of Energy (DOE) Light Water Reactor Sustainability (LWRS) Program. In the traditional probabilistic risk assessment (PRA) methodology, evaluating the potential significance of aging of passive SSCs on plant risk is challenging. Although passive SSC failure rates can be added as initiating event frequencies or basic event failure rates in the traditional event-tree/fault-tree methodology, these failure rates are generally based on generic plant failure data which means that the true state of a specific plant is not reflected in a realistic manner on aging effects. Dynamic PRA methodologies have gained attention recently due to their capability to account for the plant state and thus address the difficulties in the traditional PRA modeling of aging effects of passive components using physics-based models (and also in the modeling of digital instrumentation and control systems). Physics-based models can capture the impact of complex aging processes (e.g., fatigue, stress corrosion cracking, flow-accelerated corrosion, etc.) on SSCs and can be utilized to estimate passive SSC failure rates using realistic NPP data from reactor simulation, as well as considering effects of surveillance and maintenance activities. The objectives of this dissertation are twofold: The development of a methodology for the incorporation of aging modeling of passive SSC into a reactor simulation environment to provide a framework for evaluation of their risk contribution in both the dynamic and traditional PRA; and the demonstration of the methodology through its application to pressurizer surge line pipe weld and steam generator tubes in commercial nuclear power plants. In the proposed methodology, a

  20. Oconee Nuclear Station, Units 1, 2, and 3. Annual operating report for 1976, volume 1: nonradiological environmental surveillance report; II: summary of operations

    International Nuclear Information System (INIS)

    1977-01-01

    The non-radiological environmental surveillance program including thermal and chemical effluents, water quality, fish populations, benthos, fish impingement, gas-bubble disease, and plankton, fish larvae and fish egg entrainment is described. Information is also presented concerning operations, personnel radiation exposures, and fuel examinations

  1. French experience in transient data collection and fatigue monitoring of PWR`s nuclear steam supply system; Experience francaise sur la comptabilisation des transitoires et la surveillance en fatigue des chaudieres REP

    Energy Technology Data Exchange (ETDEWEB)

    Sabaton, M.; Morilhat, P.; Savoldelli, D.; Genette, P.

    1995-10-01

    Electricite de France (EDF), the french national electricity company, is operating 54 standardized pressurizer water reactors. This about 500 reactor-years experience in nuclear stations operation and maintenance area has allowed EDF to develop its own strategy for monitoring of age-related degradations of NPP systems and components relevant for plant safety and reliability. After more than fifteen years of experience in regulatory transient data collection and seven years of successful fatigue monitoring prototypes experimentation, EDF decided to design a new system called SYSFAC (acronym for SYsteme de Surveillance en FAtigue de la Chaudiere) devoted to transient logging and thermal fatigue monitoring of the reactor coolant pressure boundary. The system is fully automatic and directly connected to the on-site data acquisition network without any complementary instrumentation. A functional transient detection module and a mechanical transient detection module are in charge of the general transient data collection. A fatigue monitoring module is aimed towards a precise surveillance of five specific zones particularly sensible to thermal fatigue. After the first step of preliminary studies, the industrial phase of the SYSFAC project is currently going on, with hardware and software tests and implementation. The first SYSFAC system will be delivered to the pilot power plant by the beginning of 1996. The extension to all EDF`s nuclear 900 MW is planned after one more year of feedback experience. (authors). 12 refs., 3 figs.

  2. Who is Surveilling Whom?

    DEFF Research Database (Denmark)

    Mortensen, Mette

    2014-01-01

    This article concerns the particular form of counter-surveillance termed “sousveillance”, which aims to turn surveillance at the institutions responsible for surveillance. Drawing on the theoretical perspectives “mediatization” and “aerial surveillance,” the article studies WikiLeaks’ publication...

  3. Regulatory oversight report 2009 concerning nuclear safety in Swiss nuclear installations; Aufsichtsbericht 2009 zur nuklearen Sicherheit in den schweizerischen Kernanlagen/Rapport de surveillance 2009 sur la securite nucleaire dans les installations nucleaires en Suisse/Regulatory oversight report 2009 concerning nuclear safety in Swiss nuclear installations

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2010-04-15

    ENSI, the regulatory body of the Swiss Confederation, assesses and monitors the nuclear safety of nuclear facilities in Switzerland. These include the five nuclear power plants (NPPs), the plant-based interim storage facilities, the Central Interim Storage Facility (ZWILAG) at Wuerenlingen, as well as the nuclear facilities at the Paul Scherrer Institute (PSI) and the two universities of Basel and Lausanne (EPFL). Its regulatory obligations also include the transport of radioactive materials from and to nuclear facilities and the preparation of a deep geological repository for radioactive waste. ENSI maintains its own emergency organisation that would be activated in case of a serious incident at a nuclear facility in Switzerland. The legislative framework for ENSI's regulatory functions are the Nuclear Energy Act (KEG), the Nuclear Energy Ordinance (KEV), the Radiological Protection Act (StSG), the Radiological Protection Ordinance (StSV), as well as other ordinances and regulations related to reactor safety, the training of operating personnel, the organisation of the emergency response to increases in radioactivity, the transport of radioactive materials, and the deep geological repository. ENSI formulates and updates guidelines that stipulate the criteria by which it evaluates the activities and plans put forward by the operators of nuclear facilities. It regularly publishes reports and provides the public with information on events and findings at nuclear facilities. Chapters 1 to 4 of this Surveillance Report are devoted to the five Swiss NPPs. For each plant, the ENSI evaluation concludes with a safety ranking: high, good, satisfactory and unsatisfactory. Chapter 5 deals with ZWILAG for the processing and interim storage of radioactive waste from Swiss nuclear facilities. Chapters 6 and 7 deal with the nuclear facilities at PSI and with the research reactors at Basel and at EPFL. Chapter 8 deals with the transport of radioactive materials from and to

  4. Mobile Surveillance and Monitoring Robots

    International Nuclear Information System (INIS)

    Kimberly, Howard R.; Shipers, Larry R.

    1999-01-01

    Long-term nuclear material storage will require in-vault data verification, sensor testing, error and alarm response, inventory, and maintenance operations. System concept development efforts for a comprehensive nuclear material management system have identified the use of a small flexible mobile automation platform to perform these surveillance and maintenance operations. In order to have near-term wide-range application in the Complex, a mobile surveillance system must be small, flexible, and adaptable enough to allow retrofit into existing special nuclear material facilities. The objective of the Mobile Surveillance and Monitoring Robot project is to satisfy these needs by development of a human scale mobile robot to monitor the state of health, physical security and safety of items in storage and process; recognize and respond to alarms, threats, and off-normal operating conditions; and perform material handling and maintenance operations. The system will integrate a tool kit of onboard sensors and monitors, maintenance equipment and capability, and SNL developed non-lethal threat response technology with the intelligence to identify threats and develop and implement first response strategies for abnormal signals and alarm conditions. System versatility will be enhanced by incorporating a robot arm, vision and force sensing, robust obstacle avoidance, and appropriate monitoring and sensing equipment

  5. Tank Farm Operations Surveillance Automation Analysis

    International Nuclear Information System (INIS)

    MARQUEZ, D.L.

    2000-01-01

    The Nuclear Operations Project Services identified the need to improve manual tank farm surveillance data collection, review, distribution and storage practices often referred to as Operator Rounds. This document provides the analysis in terms of feasibility to improve the manual data collection methods by using handheld computer units, barcode technology, a database for storage and acquisitions, associated software, and operational procedures to increase the efficiency of Operator Rounds associated with surveillance activities

  6. Ideology, Critique and Surveillance

    Directory of Open Access Journals (Sweden)

    Heidi Herzogenrath-Amelung

    2013-11-01

    Full Text Available The 2013 revelations concerning global surveillance programmes demonstrate in unprecedented clarity the need for Critical Theory of information and communication technologies (ICTs to address the mechanisms and implications of increasingly global, ubiquitous surveillance. This is all the more urgent because of the dominance of the “surveillance ideology” (the promise of security through surveillance that supports the political economy of surveillance. This paper asks which theoretical arguments and concepts can be useful for philosophically grounding a critique of this surveillance ideology. It begins by examining how the surveillance ideology works through language and introduces the concept of the ‘ideological packaging’ of ICTs to show how rhetoric surrounding the implementation of surveillance technologies reinforces the surveillance ideology. It then raises the problem of how ideology-critique can work if it relies on language itself and argues that Martin Heidegger’s philosophy can make a useful contribution to existing critical approaches to language.

  7. Equipment for the conditioning of core components in the fuel element storage pool with particular respect to the design required by the conditions for nuclear facilities in operation and the surveillance in accordance with atomic rules and regulations

    International Nuclear Information System (INIS)

    Dumpe, J.; Schwiertz, V.; Geiser, C.; Prucker, E.

    2001-01-01

    In nuclear power plants worn out and activated parts from the reactor core (core components) which are placed into the fuel element storage pool arise on a regular basis during the technical maintenance and the review. The disposal of these core components due to radiation protection aspects is only feasible within the fuel element storage pool during the operation of the NPP using techniques of the under water conditioning. Therefore, special GNS equipment is used for the conditioning, using under water conditioning equipment, such as UWS, BZ, and ZVA, a number of lifting and auxiliary equipment for mounting and dismantling purposes and the handling of the core components and the waste casks within the fuel element storage pool. These components must meet particular safety requirements with regard to their integrity and reliability. They are designed according to the requirements on nuclear components (KTA). The manipulating equipment must be partly redundant and the protection goals for nuclear accidents must be met. The Bavarian Ministry for Development and Environment tasked the TUeV Sueddeutschland with the surveillance and control. The conditioning equipment of GNS is therefore designed in co-ordination with the examiner of the Governmental Regulating Agency, in particular respect to all safety aspects. Furthermore the examiners perform reviews of the construction and the documentation during the design and construction phase. (orig.)

  8. SOA-surveillance Nederland

    NARCIS (Netherlands)

    Rijlaarsdam J; Bosman A; Laar MJW van de; CIE

    2000-01-01

    In May 1999 a working group was started to evaluate the current surveillance systems for sexually transmitted diseases (STD) and to make suggestions for a renewed effective and efficient STD surveillance system in the Netherlands. The surveillance system has to provide insight into the prevalence

  9. Containment and surveillance devices

    International Nuclear Information System (INIS)

    Campbell, J.W.; Johnson, C.S.; Stieff, L.R.

    The growing acceptance of containment and surveillance as a means to increase safeguards effectiveness has provided impetus to the development of improved surveillance and containment devices. Five recently developed devices are described. The devices include one photographic and two television surveillance systems and two high security seals that can be verified while installed

  10. Improving the reliability of open-cycle water systems: Application of biofouling surveillance and control techniques to sediment and corrosion fouling at nuclear power plants

    International Nuclear Information System (INIS)

    Johnson, K.I.; Neitzel, D.A.

    1987-03-01

    Biofouling surveillance and control techniques are evaluated for their applicability to sediment and corrosion fouling and suggestions are given to improve their effectiveness. Alternate techniques to better detect and control sedimentation and corrosion are also evaluated. Environmental conditions that allow biofouling, sedimentation, and corrosion to occur are summarized. A correlation between sediment and corrosion is identified and the causes are described. Environmental regulations, especially those in the Clean Water Act of 1977, are reviewed to identify those that may limit or prevent the use of surveillance and control techniques described in this report. Flow velocity is the major design factor that determines whether or not biofouling, sedimentation, and corrosion will occur. Monitoring flow conditions can provide early warning of conditions that will allow fouling to occur. Visual inspection is the most common and most effective technique for identifying the cause and extent of fouling in the open-cycle water system. Most biofouling control techniques in current use are not effective against sediment and corrosion. Frequent, high-velocity flushing of cooling loops may effectively remove sediment and reduce under-sediment corrosion. Alternate biocide treatments such as targeted chlorination or the use of ozone or 2,2-dibromo-3-nitrilo propionamide (DBNPA) may also be effective in reducing under-sediment corrosion

  11. SCORPIO - VVER core surveillance system

    International Nuclear Information System (INIS)

    Zalesky, K.; Svarny, J.; Novak, L.; Rosol, J.; Horanes, A.

    1997-01-01

    The Halden Project has developed the core surveillance system SCORPIO which has two parallel modes of operation: the Core Follow Mode and the Predictive Mode. The main motivation behind the development of SCORPIO is to make a practical tool for reactor operators which can increase the quality and quantity of information presented on core status and dynamic behavior. This can first of all improve plant safety as undesired core conditions are detected and prevented. Secondly, more flexible and efficient plant operation is made possible. So far the system has only been implemented on western PWRs but the basic concept is applicable to a wide range of reactor including WWERs. The main differences between WWERs and typical western PWRs with respect to core surveillance requirements are outlined. The development of a WWER version of SCORPIO was initiated in cooperation with the Nuclear Research Institute at Rez and industry partners in the Czech Republic. The first system will be installed at the Dukovany NPP. (author)

  12. Remote container monitoring and surveillance systems

    International Nuclear Information System (INIS)

    Resnik, W.M.; Kadner, S.P.

    1995-01-01

    Aquila Technologies Group is developing a monitoring and surveillance system to monitor containers of nuclear materials. The system will both visually and physically monitor the containers. The system is based on the combination of Aquila's Gemini All-Digital Surveillance System and on Aquila's AssetLAN trademark asset tracking technology. This paper discusses the Gemini Digital Surveillance system as well as AssetLAN technology. The Gemini architecture with emphasis on anti-tamper security features is also described. The importance of all-digital surveillance versus other surveillance methods is also discussed. AssetLAN trademark technology is described, emphasizing the ability to continually track containers (as assets) by location utilizing touch memory technology. Touch memory technology provides unique container identification, as well as the ability to store and retrieve digital information on the container. This information may relate to container maintenance, inspection schedules, and other information. Finally, this paper describes the combination of the Gemini system with AssetLAN technology, yielding a self contained, container monitoring and area/container surveillance system. Secure container fixture design considerations are discussed. Basic surveillance review functions are also discussed

  13. Performance indicators for rinderpest surveillance

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-12-01

    In 1986, the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture initiated a programme of assistance to FAO and IAEA Member States for the development of effective, quality assured veterinary laboratory diagnostic services. This programme introduced the use of standardized and internationally validated ELISA-based systems for the diagnosis and surveillance of the major transboundary diseases that affect livestock. This approach has proved of immense value in the monitoring of national, regional and global animal disease control and eradication programmes. One such programme focuses on the global elimination of rinderpest. Co-ordinated by FAO through the Global Rinderpest Eradication Programme (GREP) the joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture has developed critical diagnostic and epidemiological tools to assist this effort. As the final stages of the global eradication of rinderpest are reached, it is fitting that the Joint Division should again take the lead in providing guidance to Member States on how best to meet the criteria for quality assurance of national disease surveillance programmes - a prerequisite for international acceptance of freedom from a particular disease. This publication is intended to provide countries involved in rinderpest eradication with a detailed protocol for using performance indicators in evaluating their disease surveillance system and making, where necessary, adjustments to meet the criteria for acceptance specified in the OIE Rinderpest Pathway - a pathway that leads to international recognition of freedom from rinderpest. An initial publication (IAEA-TECDOC-1161) described guidelines for the use of performance indicators in rinderpest surveillance programmes. This publication now describes in detail the protocols and the linked indicators which have been developed and field validated through a series of FAO/IAEA meetings and through IAEA expert assignments to countries in Africa.

  14. Performance indicators for rinderpest surveillance

    International Nuclear Information System (INIS)

    2001-12-01

    In 1986, the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture initiated a programme of assistance to FAO and IAEA Member States for the development of effective, quality assured veterinary laboratory diagnostic services. This programme introduced the use of standardized and internationally validated ELISA-based systems for the diagnosis and surveillance of the major transboundary diseases that affect livestock. This approach has proved of immense value in the monitoring of national, regional and global animal disease control and eradication programmes. One such programme focuses on the global elimination of rinderpest. Co-ordinated by FAO through the Global Rinderpest Eradication Programme (GREP) the joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture has developed critical diagnostic and epidemiological tools to assist this effort. As the final stages of the global eradication of rinderpest are reached, it is fitting that the Joint Division should again take the lead in providing guidance to Member States on how best to meet the criteria for quality assurance of national disease surveillance programmes - a prerequisite for international acceptance of freedom from a particular disease. This publication is intended to provide countries involved in rinderpest eradication with a detailed protocol for using performance indicators in evaluating their disease surveillance system and making, where necessary, adjustments to meet the criteria for acceptance specified in the OIE Rinderpest Pathway - a pathway that leads to international recognition of freedom from rinderpest. An initial publication (IAEA-TECDOC-1161) described guidelines for the use of performance indicators in rinderpest surveillance programmes. This publication now describes in detail the protocols and the linked indicators which have been developed and field validated through a series of FAO/IAEA meetings and through IAEA expert assignments to countries in Africa

  15. Evolution of RLSB, a nuclear-encoded S1 domain RNA binding protein associated with post-transcriptional regulation of plastid-encoded rbcL mRNA in vascular plants.

    Science.gov (United States)

    Yerramsetty, Pradeep; Stata, Matt; Siford, Rebecca; Sage, Tammy L; Sage, Rowan F; Wong, Gane Ka-Shu; Albert, Victor A; Berry, James O

    2016-06-29

    RLSB, an S-1 domain RNA binding protein of Arabidopsis, selectively binds rbcL mRNA and co-localizes with Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) within chloroplasts of C3 and C4 plants. Previous studies using both Arabidopsis (C3) and maize (C4) suggest RLSB homologs are post-transcriptional regulators of plastid-encoded rbcL mRNA. While RLSB accumulates in all Arabidopsis leaf chlorenchyma cells, in C4 leaves RLSB-like proteins accumulate only within Rubisco-containing bundle sheath chloroplasts of Kranz-type species, and only within central compartment chloroplasts in the single cell C4 plant Bienertia. Our recent evidence implicates this mRNA binding protein as a primary determinant of rbcL expression, cellular localization/compartmentalization, and photosynthetic function in all multicellular green plants. This study addresses the hypothesis that RLSB is a highly conserved Rubisco regulatory factor that occurs in the chloroplasts all higher plants. Phylogenetic analysis has identified RLSB orthologs and paralogs in all major plant groups, from ancient liverworts to recent angiosperms. RLSB homologs were also identified in algae of the division Charophyta, a lineage closely related to land plants. RLSB-like sequences were not identified in any other algae, suggesting that it may be specific to the evolutionary line leading to land plants. The RLSB family occurs in single copy across most angiosperms, although a few species with two copies were identified, seemingly randomly distributed throughout the various taxa, although perhaps correlating in some cases with known ancient whole genome duplications. Monocots of the order Poales (Poaceae and Cyperaceae) were found to contain two copies, designated here as RLSB-a and RLSB-b, with only RLSB-a implicated in the regulation of rbcL across the maize developmental gradient. Analysis of microsynteny in angiosperms revealed high levels of conservation across eudicot species and for both paralogs in

  16. Atmospheric surveillance self-propelling device

    International Nuclear Information System (INIS)

    Cartoux, Gerard.

    1980-11-01

    The atmospheric surveillance self-propelling device of the Saclay Nuclear Research Center can, by its conception (autonomy, rapid put into service, multiplicity of sampling and measurements), be used for all kind of measuring campains: pollution radioactive or not, routine or accidental situation, technical and logistic support and as a coordination or investigation vehicle [fr

  17. Laboratory of environmental radiological surveillance

    International Nuclear Information System (INIS)

    Mendez G, A.; Marcial M, F.; Giber F, J.; Montiel R, E.; Leon del V, E.; Rivas C, I.; Leon G, M.V.; Lagunas G, E.; Aragon S, R.; Juarez N, A.; Alfaro L, M.M.

    1991-12-01

    The department of radiological protection of the ININ requests the collaboration of the Engineering Unit for the elaboration of the work project of the laboratory of environmental radiological surveillance. The emission of radioactive substances to the atmosphere like consequence of the normal operation of the Nuclear Center, constitutes an exhibition source from the man to the radiations that it should be appropriately watched over and controlled to be able to determine the population's potential exhibition that it lives in the area of influence of the installation. (Author)

  18. Industrial installation surveillance acoustic device

    International Nuclear Information System (INIS)

    Marini, Jean; Audenard, Bernard.

    1981-01-01

    The purpose of this invention is the detection of possible impacts of bodies migrating inside the installation, using acoustic sensors of the waves emitted at the time of impact of the migrating bodies. This device makes it possible to take into account only those acoustic signals relating to the impacts of bodies migrating in the area under surveillance, to the exclusion of any other acoustic or electric perturbing phenomenon. The invention has a preferential use in the case of a linear shape installation in which a fluid flows at high rate, such as a section of the primary system of a pressurized water nuclear reactor [fr

  19. The study of the irradiation-induced embrittlement of reactor pressure vessels. Analysis of surveillance test specimens of a commercial nuclear reactor pressure vessel studied by three-dimensional atom probe and positron annihilation

    International Nuclear Information System (INIS)

    Nagai, Yasuyoshi; Toyama, Takeshi; Hasegawa, Masayuki

    2007-01-01

    The study of embrittlement of nuclear power reactor pressure vessels (RPVs) is of critical importance for the safety assessment in the nuclear industry. Some origins of embrittlement are attributed to fine Cu precipitates, matrix defects, grain boundary segregation of P and late blooming phase. This review article described nanostructural observation by three-dimensional atom probe (3DAP) and positron annihilation spectroscopy (PAS). The density and sizes of Cu-rich nanoprecipitates and grain boundary segregation are sensitively detected by 3DAP, and vacancies are probed by PAS. Element analysis around vacancies and fine microstructural Cu precipitates not containing vacancies are successfully observed by a coincidence doppler broadening method. The nanostructural evolution of irradiation-induced Cu-rich nanoprecipitates (CRNPs) and vacancy clusters in surveillance test specimens of commercial nuclear reactor pressure vessel steel welds of Doel-2 in Belgium were revealed by combining 3DAP and PAS. In both medium (0.13 wt%) and high (0.30 wt%) Cu welds, the CRNPs were found to form readily at the very beginning of the reactor lifetime. On the other hand, small vacancy clusters start appearing after the initial Cu precipitates and accumulate steadily with increasing neutron dose. The CRNPs were also observed at very low dose rate of neutrons in the test specimen of Calder Hall Reactor of Japan Atomic Power Company. The significant enhancement of these Cu precipitates results in the embrittlement in practical RPVs. At very high dose of 2.2x10 18 n/cm 2 by JMTR, the Cu precipitates were scarcely observed, and the irradiation-induced embrittlement was primarily caused from vacancy-impurity complexes and dislocation loops. (author)

  20. Physical change in cytoplasmic messenger ribonucleoproteins in cells treated with inhibitors of mRNA transcription

    International Nuclear Information System (INIS)

    Dreyfuss, G.; Adam, S.A.; Choi, Y.D.

    1984-01-01

    Exposure of intact cells to UV light brings about cross-linking of polyadenylated mRNA to a set of cytoplasmic proteins which are in direct contact with the mRNA in vivo. Substantial amounts of an additional protein of molecular weight 38,000 become cross-linked to the mRNA when cells are treated with inhibitors of mRNA synthesis (actinomycin D, camptothecin, and 5,6-dichloro-1-beta-D-ribofuranosyl benzimidazole) or after infection with vesicular stomatitis virus. Cordycepin, which inhibits polyadenylation but not mRNA synthesis, has no such effect. Inhibitors of protein synthesis and of rRNA synthesis are also without effect on 38K cross-linking to mRNA. The onset of the effect of inhibitors of mRNA synthesis on the UV cross-linkable interaction between mRNA and 38K is rapid and reaches a maximal level in less than 60 min, and it is completely and rapidly reversible. In cells treated with actinomycin D, the amount of 38K which becomes cross-linked to mRNA is proportional to the extent of inhibition of mRNA synthesis. The association of 38K with mRNA during transcriptional arrest does not require protein synthesis because simultaneous treatment with the protein synthesis inhibitor emetine does not interfere with it. The effectors which promote the interaction of 38K with mRNA do not affect the proteins which are in contact with polyadenylated heterogeneous nuclear RNA and do not markedly affect protein synthesis in the cell. The 38K protein can be isolated with the polyribosomal polyadenylated fraction from which it was purified, and monoclonal antibodies against it were prepared

  1. Redefining syndromic surveillance

    Directory of Open Access Journals (Sweden)

    Rebecca Katz

    2011-12-01

    Full Text Available With growing concerns about international spread of disease and expanding use of early disease detection surveillance methods, the field of syndromic surveillance has received increased attention over the last decade. The purpose of this article is to clarify the various meanings that have been assigned to the term syndromic surveillance and to propose a refined categorization of the characteristics of these systems. Existing literature and conference proceedings were examined on syndromic surveillance from 1998 to 2010, focusing on low- and middle-income settings. Based on the 36 unique definitions of syndromic surveillance found in the literature, five commonly accepted principles of syndromic surveillance systems were identified, as well as two fundamental categories: specific and non-specific disease detection. Ultimately, the proposed categorization of syndromic surveillance distinguishes between systems that focus on detecting defined syndromes or outcomes of interest and those that aim to uncover non-specific trends that suggest an outbreak may be occurring. By providing an accurate and comprehensive picture of this field’s capabilities, and differentiating among system types, a unified understanding of the syndromic surveillance field can be developed, encouraging the adoption, investment in, and implementation of these systems in settings that need bolstered surveillance capacity, particularly low- and middle-income countries.

  2. Airborne Video Surveillance

    National Research Council Canada - National Science Library

    Blask, Steven

    2002-01-01

    The DARPA Airborne Video Surveillance (AVS) program was established to develop and promote technologies to make airborne video more useful, providing capabilities that achieve a UAV force multiplier...

  3. Handbook of surveillance technologies

    CERN Document Server

    Petersen, JK

    2012-01-01

    From officially sanctioned, high-tech operations to budget spy cameras and cell phone video, this updated and expanded edition of a bestselling handbook reflects the rapid and significant growth of the surveillance industry. The Handbook of Surveillance Technologies, Third Edition is the only comprehensive work to chronicle the background and current applications of the full-range of surveillance technologies--offering the latest in surveillance and privacy issues.Cutting-Edge--updates its bestselling predecessor with discussions on social media, GPS circuits in cell phones and PDAs, new GIS s

  4. Concurrent use of data base and graphics computer workstations to provide graphic access to large, complex data bases for robotics control of nuclear surveillance and maintenance

    International Nuclear Information System (INIS)

    Dalton, G.R.; Tulenko, J.S.; Zhou, X.

    1990-01-01

    The University of Florida is part of a multiuniversity research effort, sponsored by the US Department of Energy which is under way to develop and deploy an advanced semi-autonomous robotic system for use in nuclear power stations. This paper reports on the development of the computer tools necessary to gain convenient graphic access to the intelligence implicit in a large complex data base such as that in a nuclear reactor plant. This program is integrated as a man/machine interface within the larger context of the total computerized robotic planning and control system. The portion of the project described here addresses the connection between the three-dimensional displays on an interactive graphic workstation and a data-base computer running a large data-base server program. Programming the two computers to work together to accept graphic queries and return answers on the graphic workstation is a key part of the interactive capability developed

  5. Nuclear

    International Nuclear Information System (INIS)

    2014-01-01

    This document proposes a presentation and discussion of the main notions, issues, principles, or characteristics related to nuclear energy: radioactivity (presence in the environment, explanation, measurement, periods and activities, low doses, applications), fuel cycle (front end, mining and ore concentration, refining and conversion, fuel fabrication, in the reactor, back end with reprocessing and recycling, transport), the future of the thorium-based fuel cycle (motivations, benefits and drawbacks), nuclear reactors (principles of fission reactors, reactor types, PWR reactors, BWR, heavy-water reactor, high temperature reactor of HTR, future reactors), nuclear wastes (classification, packaging and storage, legal aspects, vitrification, choice of a deep storage option, quantities and costs, foreign practices), radioactive releases of nuclear installations (main released radio-elements, radioactive releases by nuclear reactors and by La Hague plant, gaseous and liquid effluents, impact of releases, regulation), the OSPAR Convention, management and safety of nuclear activities (from control to quality insurance, to quality management and to sustainable development), national safety bodies (mission, means, organisation and activities of ASN, IRSN, HCTISN), international bodies, nuclear and medicine (applications of radioactivity, medical imagery, radiotherapy, doses in nuclear medicine, implementation, the accident in Epinal), nuclear and R and D (past R and D programmes and expenses, main actors in France and present funding, main R and D axis, international cooperation)

  6. Surveyor mobile surveillance system for hazardous environments

    International Nuclear Information System (INIS)

    Silverman, E.B.; Simmons, R.K.; Kniazewycz, B.G.; Darvish, A.R.; Irving, T.L.

    1987-01-01

    A successful program was recently conducted to test and evaluate a commercial-ready, wireless, remotely operated surveillance system for use in nuclear power plants. This evaluation of the Surveyor mobile surveillance system took place at Niagara Mohawk Power Corporation's Nine Mile Point Nuclear Power Station. The remotely operated vehicle measures radiation, temperature and relative humidity and provides optical inspection capability. The vehicle is readily maneuvered in 36-inch wide passageways and labyrinth entries and can climb stairs, negotiating 180-degree turns on stair landings. The system consists of a supervisory control station and a rugged, remotely-operated, battery-powered vehicle. The surveyor system is specifically designed to decrease personnel radiation exposure by supplementing the functions of an auxiliary operator or wealth physics technician to perform periodic component inspections inside particular areas within a nuclear power plant

  7. Soil and vegetation surveillance

    Energy Technology Data Exchange (ETDEWEB)

    Antonio, E.J.

    1995-06-01

    Soil sampling and analysis evaluates long-term contamination trends and monitors environmental radionuclide inventories. This section of the 1994 Hanford Site Environmental Report summarizes the soil and vegetation surveillance programs which were conducted during 1994. Vegetation surveillance is conducted offsite to monitor atmospheric deposition of radioactive materials in areas not under cultivation and onsite at locations adjacent to potential sources of radioactivity.

  8. Between visibility and surveillance

    DEFF Research Database (Denmark)

    Uldam, Julie

    As activists move from alternative media platforms to commercial social media platforms they face increasing challenges in protecting their online security and privacy. While government surveillance of activists is well-documented in both scholarly research and the media, corporate surveillance...

  9. Reassembling Surveillance Creep

    DEFF Research Database (Denmark)

    Bøge, Ask Risom; Lauritsen, Peter

    2017-01-01

    We live in societies in which surveillance technologies are constantly introduced, are transformed, and spread to new practices for new purposes. How and why does this happen? In other words, why does surveillance “creep”? This question has received little attention either in theoretical developm......We live in societies in which surveillance technologies are constantly introduced, are transformed, and spread to new practices for new purposes. How and why does this happen? In other words, why does surveillance “creep”? This question has received little attention either in theoretical...... development or in empirical analyses. Accordingly, this article contributes to this special issue on the usefulness of Actor-Network Theory (ANT) by suggesting that ANT can advance our understanding of ‘surveillance creep’. Based on ANT’s model of translation and a historical study of the Danish DNA database......, we argue that surveillance creep involves reassembling the relations in surveillance networks between heterogeneous actors such as the watchers, the watched, laws, and technologies. Second, surveillance creeps only when these heterogeneous actors are adequately interested and aligned. However...

  10. Risk effectiveness evaluation of surveillance testing

    International Nuclear Information System (INIS)

    Kim, I.S.; Samanta, P.K.; Martorell, S.; Vesely, W.E.

    1991-01-01

    To address the concerns about nuclear power plant surveillance tests, i.e., their adverse safety impact due to negative effects and too burdensome requirements, it is necessary to evaluate the safety significance or risk effectiveness of such tests explicitly considering both negative and positive effects. This paper defines the negative effects of surveillance testing from a risk perspective, and then presents a methodology to quantify the negative risk impact, i.e., the risk penalty or risk increase caused by the test. The method focuses on two important kinds of negative effects, namely, test-caused transients and test-caused equipment degradations. The concepts and quantitative methods for the risk evaluation can be used in the decision-making process to establish the safety significance of the tests and to screen the plant-specific surveillance test requirements. 6 refs., 2 figs., 2 tabs

  11. Visual interface for the automation of the instrumented pendulum of Charpy tests used in the surveillance program of reactors vessel of nuclear power plants; Interfase visual para la automatizacion del pendulo instrumentado de pruebas Charpy utilizado en el programa de vigilancia de la vasija de reactores de centrales nucleares

    Energy Technology Data Exchange (ETDEWEB)

    Rojas S, A.S.; Sainz M, E.; Ruiz E, J.A. [ININ, Carretera Mexico-Toluca Km.36.5, Mpio. de Ocoyoacac, Estado de Mexico (Mexico)]. E-mail: asrs@nuclear.inin.mx; esm@nuclear.inin.mx; jare@nuclear.inin.mx

    2004-07-01

    Inside the Programs of Surveillance of the nuclear power stations periodic information is required on the state that keep the materials with those that builds the vessel of the reactor. This information is obtained through some samples or test tubes that are introduced inside the core of the reactor and it is observed if its physical characteristics remain after having been subjected to the radiation changes and temperature. The rehearsal with the instrumented Charpy pendulum offers information on the behavior of fracture dynamics of a material. In the National Institute of Nuclear Research (ININ) it has an instrumented Charpy pendulum. The operation of this instrument is manual, having inconveniences to carry out rehearsals with radioactive material, handling of high and low temperatures, to fulfill the normative ones for the realization of the rehearsals, etc. In this work the development of a computational program is presented (virtual instrument), for the automation of the instrumented pendulum. The system has modules like: Card of data acquisition, signal processing, positioning system, tempered system, pneumatic system, compute programs like it is the visual interface for the operation of the instrumented Charpy pendulum and the acquisition of impact signals. This system shows that given the characteristics of the nuclear industry with radioactive environments, the virtual instrumentation and the automation of processes can contribute to diminish the risks to the personnel occupationally exposed. (Author)

  12. Tritium analysis in environmental samples around Nuclear Power Plants and nationwide surveillance of radionuclides in some environmental samples(meat and drinking water)

    Energy Technology Data Exchange (ETDEWEB)

    Cho, Yong Woo; Han, Man Jung; Cho, Seong Won; Cho, Hong Jun; Oh, Hyeon Kyun; Lee, Jeong Min; Chang, Jae Sook [KORTIC, Taejon (Korea, Republic of)

    2001-12-15

    12 kind of environmental samples such as soil, underground water, seawater, etc. around the Nuclear Power Plants(NPP) and surface seawater around the Korea peninsula were sampled, For the samples of rain, pine-needle, air, seawater, underground water, chinese cabbage, grain of rice and milk sampled around NPP, and surface seawater and rain sampled all around country, tritium concentration was measured, The tritium concentration in the tap water and the gamma activity in the domestic and imported beef that were sampled at ward in the large city in Korea(Seoul, Pusan, Taegu, Taejun, Inchun, Kwangju) were analyzed for the meat and drinking waters. As the results of analyzing, tritium concentration in rain and tap water were very low all around country, but a little higher around the NPP than general surrounding. At the Wolsung NPP, tritium concentration was descend according to distance from the stack. Tritium activity of surface seawater around the Korea peninsula was also, very low. The measured radioactive elements in the beef is the same as the radioactive elements on the earth surface.

  13. The Copyright Surveillance Industry

    Directory of Open Access Journals (Sweden)

    Mike Zajko

    2015-09-01

    Full Text Available Creative works are now increasingly distributed as digital “content” through the internet, and copyright law has created powerful incentives to monitor and control these flows. This paper analyzes the surveillance industry that has emerged as a result. Copyright surveillance systems identify copyright infringement online and identify persons to hold responsible for infringing acts. These practices have raised fundamental questions about the nature of identification and attribution on the internet, as well as the increasing use of algorithms to make legal distinctions. New technologies have threatened the profits of some media industries through copyright infringement, but also enabled profitable forms of mass copyright surveillance and enforcement. Rather than a system of perfect control, copyright enforcement continues to be selective and uneven, but its broad reach results in systemic harm and provides opportunities for exploitation. It is only by scrutinizing copyright surveillance practices and copyright enforcement measures that we can evaluate these consequences.

  14. Deployment Health Surveillance

    National Research Council Canada - National Science Library

    DeNicola, Anthony D

    2004-01-01

    ... of stress in causing chronic illness. The lack of comprehensive deployment health surveillance has made it difficult to determine possible causes of adverse health effects reported by Gulf War veterans...

  15. 522 Postmarket Surveillance Studies

    Data.gov (United States)

    U.S. Department of Health & Human Services — The 522 Postmarket Surveillance Studies Program encompasses design, tracking, oversight, and review responsibilities for studies mandated under section 522 of the...

  16. Sanitary surveillance and bioethics

    Directory of Open Access Journals (Sweden)

    Volnei Garrafa

    2017-08-01

    Full Text Available Regulatory practices in the field of health surveillance are indispensable. The aim of this study is to show ‒ taking the Brazilian National Surveillance Agency, governing body of sanitary surveillance in Brazil as a reference ‒ that bioethics provides public bodies a series of theoretical tools from the field of applied ethics for the proper exercise and control of these practices. To that end, the work uses two references of bioethics for the development of a comparative and supportive analysis to regulatory activities in the field of health surveillance: the Universal Declaration on Bioethics and Human Rights of Unesco and the theory of intervention bioethics. We conclude that organizations and staff working with regulatory activities can take advantage of the principles and frameworks proposed by bioethics, especially those related to the Declaration and the theory of intervention bioethics, the latter being set by the observation and use of the principles of prudence, precaution, protection and prevention.

  17. Demographic surveillance and health status of population within 0-5 km radius of proposed nuclear power plant at village Gorakhpur, District Fatehabad, Haryana, India

    International Nuclear Information System (INIS)

    Singh, Takdir; Nimble, Sarina; Jyotsana; Garg, Vinod Kumar; Narayanan, Usha

    2012-01-01

    Generation of power is a fundamental catalyst to the economic development of a country. India needs more power in order to have a strong industrial base and for infrastructure development. It is essential to take a baseline of the target area with an objective to know demographic details, health status of the area. This study was conducted to find out the demographic details and health status of the population within 0-5 km radius of proposed Nuclear Power Plant (NPP) site covering five villages of Fatehabad and Hisar districts. The study methodology includes Interview Schedule for data collection. Of the total population from 4501 households covered, 51.60% were male and 48.40% were females. The total population of the study area is 24415. The core observation in the study area pertaining to demographic and health status studies indicate that the literacy rate is 62.92% and mostly population is literate up to metric (50.16%), mostly population falls in the age group between 11-30 years, unemployment status of population is 70.50%. Most prevalent diseases at the time of study were Fever (6.10%), Cold/Cough (7.07%), Wounds (5 .22%), Irritation/Skin rashes (1.62%) and Respiratory Problems (2.36%). Talking about other important diseases like Cancer (0.0002.86%), Congenital Malformation (0.0002.86%), Deaf and dumb (0.0011%), Mental Retardation (0.0007%) and Polio are found (0.0006%) out of total population. So in the nut shell it can be concluded that the literacy rate is good and most of the surveyed population is healthy and there are no serious health problems in the area related to health point of view. (author)

  18. The Argentine remote monitoring and surveillance system

    International Nuclear Information System (INIS)

    Bonino, A.; Roca, J.L.; Perez, A.; Pizarro, L.; Krimer, M.; Teira, R.; Higa, Z.; Saettone, S.; Monzon, J.; Moroni, D.

    1996-01-01

    The Scientific and Technical Support Department of the Argentine National Board of Nuclear Regulation (ENREN) has developed a Remote Monitoring and Surveillance System (RMSS) that provides a media to verify state of variables related to the monitoring and surveillance activities of nuclear facilities, mainly safeguard applications. RMSS includes a variety of on site installed sensors, an authenticated radiofrequency communication link, a receiver processing unit, an active vision set and a user friendly personal computer interface to collect, view and store pertinent histories of events. A real time data base allows consulting, maintenance, updating and checking activities. RMSS could be integrated into a LAN or WAN via modem for use in a remote operation scheme. In this paper a description of the RMSS is provided. Also, an overview of the RMSS operation at one facility under safeguards belonging to the National Commission of Atomic Energy (CNEA) is presented. Results and conclusions of the system associated with this facility are given. (author). 37 figs

  19. The Argentine remote monitoring and surveillance system

    Energy Technology Data Exchange (ETDEWEB)

    Bonino, A; Roca, J L; Perez, A; Pizarro, L; Krimer, M; Teira, R; Higa, Z; Saettone, S; Monzon, J; Moroni, D [Ente Nacional Regulador Nuclear, Buenos Aires (Argentina). Dept. Apoyo Cientifico y Tecnico

    1997-12-31

    The Scientific and Technical Support Department of the Argentine National Board of Nuclear Regulation (ENREN) has developed a Remote Monitoring and Surveillance System (RMSS) that provides a media to verify state of variables related to the monitoring and surveillance activities of nuclear facilities, mainly safeguard applications. RMSS includes a variety of on site installed sensors, an authenticated radiofrequency communication link, a receiver processing unit, an active vision set and a user friendly personal computer interface to collect, view and store pertinent histories of events. A real time data base allows consulting, maintenance, updating and checking activities. RMSS could be integrated into a LAN or WAN via modem for use in a remote operation scheme. In this paper a description of the RMSS is provided. Also, an overview of the RMSS operation at one facility under safeguards belonging to the National Commission of Atomic Energy (CNEA) is presented. Results and conclusions of the system associated with this facility are given. (author). 37 figs.

  20. SCORPIO - WWER core surveillance system

    International Nuclear Information System (INIS)

    Hornaes, Arne; Bodal, Terje; Sunde, Svein; Zalesky, K.; Lehman, M.; Pecka, M.; Svarny, J.; Krysl, V.; Juzova, Z.; Sedlak, A.; Semmler, M.

    1998-01-01

    The Institut for energiteknikk has developed the core surveillance system SCORPIO, which has two parallel modes of operation: the Core Follow Mode and the Predictive Mode. The main motivation behind the development of SCORPIO is to make a practical tool for reactor operators which can increase the quality and quantity of information presented on core status and dynamic behavior. This can first of all improve plant safety, as undesired core conditions are detected and prevented. Secondly, more flexible and efficient plant operation is made possible. The system has been implemented on western PWRs, but the basic concept is applicable to a wide range of reactors including WWERs. The main differences between WWERs and typical western PWRs with respect to core surveillance requirements are outlined. The development of a WWER version of SCORPIO has been done in co-operation with the Nuclear Research Institute Rez, and industry partners in the Czech Republic. The first system is installed at Dukovany NPP, where the Site Acceptance Test was completed 6. March 1998.(Authors)

  1. SCORPIO - VVER core surveillance system

    International Nuclear Information System (INIS)

    Hornaes, A.; Bodal, T.; Sunde, S.

    1998-01-01

    The Institutt for energiteknikk has developed the core surveillance system SCORPIO, which has two parallel modes of operation: the Core Follow Mode and the Predictive Mode. The main motivation behind the development of SCORPIO is to make a practical tool for reactor operators, which can increase the quality and quantity of information presented on core status and dynamic behavior. This can first of all improve plant safety, as undesired core conditions are detected and prevented. Secondly, more flexible and efficient plant operation is made possible. The system has been implemented on western PWRs, but the basic concept is applicable to a wide range of reactors including VVERs. The main differences between VVERs and typical western PWRs with respect to core surveillance requirements are outlined. The development of a VVER version of SCORPIO has been done in co-operation with the Nuclear Research Institute Rez, and industry partners in the Czech Republic. The first system is installed at Dukovany NPP, where the Site Acceptance Test was completed 6. March 1998.(author)

  2. Radiation detectors as surveillance monitors

    International Nuclear Information System (INIS)

    Fehlau, P.E.; Dowdy, E.J.

    1981-01-01

    The International Atomic Energy Agency (IAEA) proposes to use personnel dosimetry radiation detectors as surveillance monitors for safeguards purposes. It plans to place these YES/NO monitors at barrier penetration points declared closed under IAEA safeguards to detect the passage of plutonium-bearing nuclear material, usually spent fuel. For this application, commercially available dosimeters were surveyed as well as other radiation detectors that appeared suitable and likely to be marketed in the near future. No primary advantage was found in a particular detector type because in this application backgrounds vary during long counting intervals. Secondary considerations specify that the monitor be inexpensive and easy to tamper-proof, interrogate, and maintain. On this basis radiophotoluminescent, thermoluminescent, and electronic dosimeters were selected as possible routine monitors; the latter two may prove useful for data-base acquisition

  3. The utility industry and reactor surveillance

    International Nuclear Information System (INIS)

    Jenkins, R.B.

    1983-01-01

    Every commercial nuclear power reactor pressure vessel (RPV) is required to have a reactor vessel surveillance program at the time of plant licensing. The program is part of a continuing structural integrity assessment of the RPV. As such, the surveillance program supplements Section III of the American Society of Mechanical Engineers (ASME) Code (1), which is the design basis for nuclear power plant component pressure boundaries. The Code assumes that the materials of construction are ductile in the evaluation and design of all components. The surveillance program for each RPV is intended to provide assurance of continued applicability of the ASME Code, Appendix G, assessment of that RPV's operating limits. This assessment ensures that the RPV is always in a condition which precludes the unstable propagation of flaws in the vessel wall material. The potential presence of flaws and the desire to ensure ductility are significant considerations in ferritic steels such as those used to fabricate nuclear reactor pressure vessels. These materials are known to exhibit transition from ductile-to-brittle fracture behavior over a determined temperature range. Neutron irradiation tends to shift this ductile-to-brittle behavior transition zone to a temperature higher than unirradiated materials

  4. Research and experience report 2009. Developments in the technical and legal basis of nuclear oversight; Erfahrungs- und Forschungsbericht 2009. Entwicklungen im Bereich der Grundlagen der nuklearen Aufsicht/Rapport sur la recherche et les experiences en 2009. Developpements dans les bases techniques et legales pour la surveillance nucleaire/Research and experience report 2009. Developments in the technical and legal basis of nuclear oversight

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2010-04-15

    physical transport processes and the management of all radioactive waste. ENSI supports research projects into the influence of human behaviour on incident management together with the development of requirement profiles for staff in the control rooms of nuclear power stations. Incidents in nuclear facilities provide concrete data on shortcomings and the potential for improvements in all areas of design and operation. Information on incidents in Swiss nuclear facilities is included in ENSI's (separate) Surveillance Report. Some incidents in nuclear facilities outside Switzerland were analysed in order to identify any relevance for Swiss nuclear facilities. There has been increase worldwide in the number of incidents involving the transport of loads. In Switzerland also, the Interim Storage Facility of Wuerenlingen AG reported an event in 2009 involving a lifting device. ENSI has increased its surveillance of cranes and other lifting devices. The Ringhals nuclear power plant (NPP) in Sweden had been operating at power for 72 days before it was discovered that the automatic initiation of the containment isolation system was not operational: the bypasses in the reactor protection system were not correctly removed. Investigations identified the absence of a clear demarcation between the responsibilities of operating and maintenance personnel. Although very improbable, a similar incident might occur in Switzerland; however it is very likely that it would be picked up during the obligatory functionality check. Nevertheless, ENSI considered it important that facilities took note of this incident. Following heavy rain, debris blocked the cooling water intake at the Cruas 4 NPP; this caused the main water sink to fail. At the Fessenheim 2 NPP, plant residues found their way into the cooling water system after the cleaning system failed. By such an incident in a Swiss NPP, the plant could be made safe because the Swiss NPPs are fed with water from their own wells or from

  5. Suboptimal processor for anomaly detection for system surveillance and diagnosis

    Energy Technology Data Exchange (ETDEWEB)

    Ciftcioglu, Oe.; Hoogenboom, J.E.; Dam, H. van

    1989-06-01

    Anomaly detection for nuclear reactor surveillance and diagnosis is described. The residual noise obtained as a result of autoregressive (AR) modelling is essential to obtain high sensitivity for anomaly detection. By means of the method of hypothesis testing a suboptimal anomaly detection processor is devised for system surveillance and diagnosis. Experiments are carried out to investigate the performance of the processor, which is in particular of interest for on-line and real-time applications.

  6. VNIIEF NMPC and A Maintenance Management Conference video surveillance

    International Nuclear Information System (INIS)

    Malone, T.

    1997-08-01

    This paper is part of ongoing Nuclear Materials Protection, Control and Accountability (NMPC and A) work with the All Russian Scientific Research Institute of Experimental Physics (VNIIEF), Sarov, Russia. The material presented in the paper is to provide guidance for the preparation of maintenance management for NMPC and A video assessment and surveillance subsystems being installed at VNIIEF. This paper discusses maintenance philosophies, performance testing, equipment inspection/setup, and record keeping for a video assessment and surveillance subsystem

  7. Nuclear

    International Nuclear Information System (INIS)

    Anon.

    2000-01-01

    The first text deals with a new circular concerning the collect of the medicine radioactive wastes, containing radium. This campaign wants to incite people to let go their radioactive wastes (needles, tubes) in order to suppress any danger. The second text presents a decree of the 31 december 1999, relative to the limitations of noise and external risks resulting from the nuclear facilities exploitation: noise, atmospheric pollution, water pollution, wastes management and fire prevention. (A.L.B.)

  8. Surveillance of antibiotic resistance

    Science.gov (United States)

    Johnson, Alan P.

    2015-01-01

    Surveillance involves the collection and analysis of data for the detection and monitoring of threats to public health. Surveillance should also inform as to the epidemiology of the threat and its burden in the population. A further key component of surveillance is the timely feedback of data to stakeholders with a view to generating action aimed at reducing or preventing the public health threat being monitored. Surveillance of antibiotic resistance involves the collection of antibiotic susceptibility test results undertaken by microbiology laboratories on bacteria isolated from clinical samples sent for investigation. Correlation of these data with demographic and clinical data for the patient populations from whom the pathogens were isolated gives insight into the underlying epidemiology and facilitates the formulation of rational interventions aimed at reducing the burden of resistance. This article describes a range of surveillance activities that have been undertaken in the UK over a number of years, together with current interventions being implemented. These activities are not only of national importance but form part of the international response to the global threat posed by antibiotic resistance. PMID:25918439

  9. Radioactive survey of the environment of the nuclear sites of French Nuclear Board: an overview; Bilan de la surveillance de la radioactivite dans l'environnement des sites du commissariat a l'energie atomique

    Energy Technology Data Exchange (ETDEWEB)

    Robeau, D; Montjoie, M; Sauve, A M; Laporte, J; Rahaplen, A; Alphonse, L; Huc, C [C.E.A./Institut de Protection et de Surete Nucleaire, Centre d' Etudes de Fontenay-aux-Roses, Fontenay-aux-Roses (France); Bensimon, C; Cissoko, G [Compagnie Internationale de Services Informatiques (France)

    1992-07-01

    C.E.A. has set up a network of radioactive survey around its nuclear sites. This network involves terrestrial, atmospheric and marine results of radioactive measurements. This survey is structured in four levels. The level 0 homogenizes stations of measurements, level 1 centralizes real-time measurements of gross {alpha} and {beta} measurements of atmospheric radioactivity, level 2 and 3 centralizes postponed {alpha}-{beta} spectrometric measurements of radioactivity on water, deposition, grass, vegetables. People can have a squint at these results of measurements using popular MINITEL telephonic network. (author)

  10. Interactions between the HIV-1 Unspliced mRNA and Host mRNA Decay Machineries

    Directory of Open Access Journals (Sweden)

    Daniela Toro-Ascuy

    2016-11-01

    Full Text Available The human immunodeficiency virus type-1 (HIV-1 unspliced transcript is used both as mRNA for the synthesis of structural proteins and as the packaged genome. Given the presence of retained introns and instability AU-rich sequences, this viral transcript is normally retained and degraded in the nucleus of host cells unless the viral protein REV is present. As such, the stability of the HIV-1 unspliced mRNA must be particularly controlled in the nucleus and the cytoplasm in order to ensure proper levels of this viral mRNA for translation and viral particle formation. During its journey, the HIV-1 unspliced mRNA assembles into highly specific messenger ribonucleoproteins (mRNPs containing many different host proteins, amongst which are well-known regulators of cytoplasmic mRNA decay pathways such as up-frameshift suppressor 1 homolog (UPF1, Staufen double-stranded RNA binding protein 1/2 (STAU1/2, or components of miRNA-induced silencing complex (miRISC and processing bodies (PBs. More recently, the HIV-1 unspliced mRNA was shown to contain N6-methyladenosine (m6A, allowing the recruitment of YTH N6-methyladenosine RNA binding protein 2 (YTHDF2, an m6A reader host protein involved in mRNA decay. Interestingly, these host proteins involved in mRNA decay were shown to play positive roles in viral gene expression and viral particle assembly, suggesting that HIV-1 interacts with mRNA decay components to successfully accomplish viral replication. This review summarizes the state of the art in terms of the interactions between HIV-1 unspliced mRNA and components of different host mRNA decay machineries.

  11. Safeguards and nuclear forensics

    International Nuclear Information System (INIS)

    Gangotra, Suresh

    2016-01-01

    Nuclear Safeguards is the detection of diversion of significant quantities of nuclear material from peaceful nuclear activities to the manufacture of nuclear weapons, or of other nuclear explosive devices or for purposes unknown, and deterrence of such diversion by early detection. Safeguards implementation involves nuclear material accounting and containment and surveillance measures. The safeguards are implemented in nuclear facilities by the states, or agencies and International Atomic Energy Agency (IAEA). The measures for the safeguards include nuclear material Accounting (NUMAC) and Containment and surveillance systems. In recent times, there have been advances in safeguards like Near Real Time Monitoring (NRTM), Dynamic Nuclear Material Accounting (DNMA), Safeguards-by-Design (SBD), satellite imagery, information from open sources, remote monitoring etc

  12. Self-amplifying mRNA vaccines.

    Science.gov (United States)

    Brito, Luis A; Kommareddy, Sushma; Maione, Domenico; Uematsu, Yasushi; Giovani, Cinzia; Berlanda Scorza, Francesco; Otten, Gillis R; Yu, Dong; Mandl, Christian W; Mason, Peter W; Dormitzer, Philip R; Ulmer, Jeffrey B; Geall, Andrew J

    2015-01-01

    This chapter provides a brief introduction to nucleic acid-based vaccines and recent research in developing self-amplifying mRNA vaccines. These vaccines promise the flexibility of plasmid DNA vaccines with enhanced immunogenicity and safety. The key to realizing the full potential of these vaccines is efficient delivery of nucleic acid to the cytoplasm of a cell, where it can amplify and express the encoded antigenic protein. The hydrophilicity and strong net negative charge of RNA impedes cellular uptake. To overcome this limitation, electrostatic complexation with cationic lipids or polymers and physical delivery using electroporation or ballistic particles to improve cellular uptake has been evaluated. This chapter highlights the rapid progress made in using nonviral delivery systems for RNA-based vaccines. Initial preclinical testing of self-amplifying mRNA vaccines has shown nonviral delivery to be capable of producing potent and robust innate and adaptive immune responses in small animals and nonhuman primates. Historically, the prospect of developing mRNA vaccines was uncertain due to concerns of mRNA instability and the feasibility of large-scale manufacturing. Today, these issues are no longer perceived as barriers in the widespread implementation of the technology. Currently, nonamplifying mRNA vaccines are under investigation in human clinical trials and can be produced at a sufficient quantity and quality to meet regulatory requirements. If the encouraging preclinical data with self-amplifying mRNA vaccines are matched by equivalently positive immunogenicity, potency, and tolerability in human trials, this platform could establish nucleic acid vaccines as a versatile new tool for human immunization. Copyright © 2015 Elsevier Inc. All rights reserved.

  13. A novel link between Sus1 and the cytoplasmic mRNA decay machinery suggests a broad role in mRNA metabolism

    Directory of Open Access Journals (Sweden)

    Llopis Ana

    2010-03-01

    Full Text Available Abstract Background Gene expression is achieved by the coordinated action of multiple factors to ensure a perfect synchrony from chromatin epigenetic regulation through to mRNA export. Sus1 is a conserved mRNA export/transcription factor and is a key player in coupling transcription initiation, elongation and mRNA export. In the nucleus, Sus1 is associated to the transcriptional co-activator SAGA and to the NPC associated complex termed TREX2/THSC. Through these associations, Sus1 mediates the nuclear dynamics of different gene loci and facilitate the export of the new transcripts. Results In this study, we have investigated whether the yeast Sus1 protein is linked to factors involved in mRNA degradation pathways. We provide evidence for genetic interactions between SUS1 and genes coding for components of P-bodies such as PAT1, LSM1, LSM6 and DHH1. We demonstrate that SUS1 deletion is synthetic lethal with 5'→3' decay machinery components LSM1 and PAT1 and has a strong genetic interaction with LSM6 and DHH1. Interestingly, Sus1 overexpression led to an accumulation of Sus1 in cytoplasmic granules, which can co-localise with components of P-bodies and stress granules. In addition, we have identified novel physical interactions between Sus1 and factors associated to P-bodies/stress granules. Finally, absence of LSM1 and PAT1 slightly promotes the Sus1-TREX2 association. Conclusions In this study, we found genetic and biochemical association between Sus1 and components responsible for cytoplasmic mRNA metabolism. Moreover, Sus1 accumulates in discrete cytoplasmic granules, which partially co-localise with P-bodies and stress granules under specific conditions. These interactions suggest a role for Sus1 in gene expression during cytoplasmic mRNA metabolism in addition to its nuclear function.

  14. Clinical significance of LUNX mRNA, CK19 mRNA, CEA mRNA expression in detecting micrometastasis from lung cancer

    International Nuclear Information System (INIS)

    Zhu Guangying; Liu Delin; Chen Jie

    2003-01-01

    Objective: To evaluate the sensitivity, specificity and clinical significance of CK19 mRNA, CEA mRNA and LUNX mRNA for detecting micrometastasis by sampling the peripheral blood and regional lymph nodes of lung cancer patients. Methods: Reverse transcriptase chain reaction (RT-PCR) was used to detect LUNX mRNA, CK19 mRNA, CEA mRNA for micrometastasis by sampling the peripheral blood of 48 lung cancer patients and 44 regional lymph nodes of such patients treated by curative resection. Peripheral blood of 30 patients with pulmonary benign lesions and 10 normal healthy volunteers and lymph nodes of 6 patients with benign pulmonary diseases served as control. Results: 1) LUNX mRNA, CK19 mRNA, CEA mRNA were expressed in all (35/35) lung cancer tissues. 2) In the peripheral blood from 48 lung cancer patients, 30 (62.5%) were positive for LUNX mRNA, 24 (50.0%) positive for CK19 mRNA and 32(66.7%) positive for CEA mRNA. The positive detection rates of micrometastasis in 44 lymph nodes from lung cancer patients were 36.4% (16 out of 44) for LUNX mRNA, 27.3% (12 out of 44) for CK19 mRNA and 40.9% (18 out of 44) for CEA mRNA. 3) In the 30 blood samples from patients with pulmonary benign diseases, 2 (6.7%) expressed CK19 mRNA, but none expressed LUNX mRNA or CEA mRNA. All the 3 molecular markers were negative in the 10 blood samples from healthy volunteers. In 11 lymph nodes from patients with pulmonary benign lesions, none was positive for any of the three markers. 4) In 44 regional lymph nodes from lung cancer patients, 6 (13.6%) were positive for metastasis by histopathological examination, with a positive rate significantly lower than that of the RT-PCR (P<0.05). 5) The micrometastatic positive rate in the peripheral blood of 40 non-small cell lung cancer (NSCLC) patients was significantly related to TNM stage (P=0.01). Conclusions: LUNX mRNA, CK19 MRNA, CEA mRNA are all appropriate target genes for the detection of micrometastasis from lung cancer. LUNX mRNA and CEA mRNA

  15. The plays and arts of surveillance: studying surveillance as entertainment

    NARCIS (Netherlands)

    Albrechtslund, Anders; Dubbeld, L.

    2006-01-01

    This paper suggests a direction in the development of Surveillance Studies that goes beyond current attention for the caring, productive and enabling aspects of surveillance practices. That is, surveillance could be considered not just as positively protective, but even as a comical, playful,

  16. Secure Video Surveillance System (SVSS) for unannounced safeguards inspections

    International Nuclear Information System (INIS)

    Galdoz, Erwin G.; Pinkalla, Mark

    2010-01-01

    The Secure Video Surveillance System (SVSS) is a collaborative effort between the U.S. Department of Energy (DOE), Sandia National Laboratories (SNL), and the Brazilian-Argentine Agency for Accounting and Control of Nuclear Materials (ABACC). The joint project addresses specific requirements of redundant surveillance systems installed in two South American nuclear facilities as a tool to support unannounced inspections conducted by ABACC and the International Atomic Energy Agency (IAEA). The surveillance covers the critical time (as much as a few hours) between the notification of an inspection and the access of inspectors to the location in facility where surveillance equipment is installed. ABACC and the IAEA currently use the EURATOM Multiple Optical Surveillance System (EMOSS). This outdated system is no longer available or supported by the manufacturer. The current EMOSS system has met the project objective; however, the lack of available replacement parts and system support has made this system unsustainable and has increased the risk of an inoperable system. A new system that utilizes current technology and is maintainable is required to replace the aging EMOSS system. ABACC intends to replace one of the existing ABACC EMOSS systems by the Secure Video Surveillance System. SVSS utilizes commercial off-the shelf (COTS) technologies for all individual components. Sandia National Laboratories supported the system design for SVSS to meet Safeguards requirements, i.e. tamper indication, data authentication, etc. The SVSS consists of two video surveillance cameras linked securely to a data collection unit. The collection unit is capable of retaining historical surveillance data for at least three hours with picture intervals as short as 1sec. Images in .jpg format are available to inspectors using various software review tools. SNL has delivered two SVSS systems for test and evaluation at the ABACC Safeguards Laboratory. An additional 'proto-type' system remains

  17. Conic surveillance evasion

    NARCIS (Netherlands)

    Lewin, J.; Olsder, G.J.

    1979-01-01

    A surveillance-evasion differential game of degree with a detection zone in the shape of a two-dimensional cone is posed. The nature of the optimal strategies and the singular phenomena of the value function are described and correlated to subsets of the space of all possible parameter combinations,

  18. Laser surveillance system (LASSY)

    International Nuclear Information System (INIS)

    Boeck, H.; Hammer, J.

    1988-01-01

    The development progress during the reporting period 1988 of the laser surveillance system of spent fuel pools is summarized. The present engineered system comes close to a final version for field application as all technical questions have been solved in 1988. 14 figs., 1 tab. (Author)

  19. Laser surveillance system (LASSY)

    International Nuclear Information System (INIS)

    Boeck, H.

    1991-09-01

    Laser Surveillance System (LASSY) is a beam of laser light which scans a plane above the water or under-water in a spent-fuel pond. The system can detect different objects and estimates its coordinates and distance as well. LASSY can operate in stand-alone configuration or in combination with a video surveillance to trigger signal to a videorecorder. The recorded information on LASSY computer's disk comprises date, time, start and stop angle of detected alarm, the size of the disturbance indicated in number of deviated points and some other information. The information given by the laser system cannot be fully substituted by TV camera pictures since the scanning beam creates a horizontal surveillance plan. The engineered prototype laser system long-term field test has been carried out in Soluggia (Italy) and has shown its feasibility and reliability under the conditions of real spent fuel storage pond. The verification of the alarm table on the LASSY computer with the recorded video pictures of TV surveillance system confirmed that all alarm situations have been detected. 5 refs

  20. Infectieziekten Surveillance Informatie Systeem

    NARCIS (Netherlands)

    Sprenger MJW; van Pelt W; CIE

    1994-01-01

    In the Netherlands an electronic network has been proposed for structured data transfer and communication concerning the control of infectious diseases. This project has been baptized ISIS (Infectious diseases Surveillance Information System). It is an initiative of the Dutch Government. ISIS

  1. Surveillance and Communication

    DEFF Research Database (Denmark)

    Bøge, Ask Risom; Albrechtslund, Anders; Lauritsen, Peter

    2017-01-01

    , and acquaintances are up to on social media. In turn, they also leave trails of digital footprints that may be collected and analyzed by governments, businesses, or hackers. The imperceptible nature of this new surveillance raises some pressing concerns about our digital lives as our data doubles increasingly...

  2. Guidelines for the medical surveillance of atomic radiation workers

    International Nuclear Information System (INIS)

    1991-11-01

    These guidelines are provided for the use and guidance of occupational physicians concerned with the medical surveillance of atomic radiation workers (ARWs). Persons employed in industries where there is exposure to ionizing radiation should be screened medically for fitness for certain jobs before starting such work and at appropriate intervals while employed. This includes workers at uranium mines, mills and refineries, nuclear fuel fabrication plants, nuclear power plants and research facilities, and facilities using radionuclides in an industrial setting. An important purpose of medical surveillance is to ensure that workers are fit both physically and psychologically to undertake the tasks they may be called upon to perform

  3. Development of Reconstitution Technology for Surveillance Specimens

    International Nuclear Information System (INIS)

    Yasushi Atago; Shunichi Hatano; Eiichiro Otsuka

    2002-01-01

    The Japan Power Engineering and Inspection Corporation (JAPEIC) has been carrying out the project titled 'Nuclear Power Plant Integrated Management Technology (PLIM)' consigned by Japanese Ministry of Economy, Trade and Industry (METI) since 1996FY as a 10-years project. As one of the project themes, development of reconstitution technology for reactor pressure vessel (RPV/RV) surveillance specimens, which are installed in RPVs to monitor the neutron irradiation embrittlement on RPV/RV materials, is now on being carried out to deal with the long-term operation of nuclear power plants. The target of this theme is to establish the technical standard for applicability of reconstituted surveillance specimens including the reconstitution of the Charpy specimens and Compact Tension (CT) specimens. With the Charpy specimen reconstitution, application of 10 mm length inserts is used, which enables the conversion of tests from the LT-direction to the TL-direction. This paper presents the basic data from Charpy and CT specimens of RPV materials using the surveillance specimens obtained for un-irradiated materials including the following. 1) Reconstitution Technology of Charpy Specimens. a) The interaction between plastic zone and Heat Affected Zone (HAZ). b) The effects of the possible deviations from the standard specimens for the reconstituted specimens. 2) Reconstitution Technology of CT specimens. a) The correlation between fracture toughness and plastic zone width. Because the project is now in progress, this paper describes the outline of the results obtained as of the end of 2000 FY. (authors)

  4. Radiological surveillance in Mexico, derived of the accident of the Fukushima Daiichi nuclear power plant; Vigilancia radiologica en Mexico, derivado del accidente en la central nuclear de Fukushima Daiichi

    Energy Technology Data Exchange (ETDEWEB)

    Aguirre G, J.; Nohpal J, X., E-mail: jaguirre@cnsns.gob.mx [Comision Nacional de Seguridad Nuclear y Salvaguardias, Departamento de Vigilancia Radiologica, Dr. Barragan No. 779, Col. Narvarte, 03020 Mexico D. F. (Mexico)

    2012-10-15

    March 11, 2011 an earthquake of 9.0 grades in the Richter scale, originated in the coast of Tohoku, Japan, in the Pacific Ocean gave origin to a tsunami that caused an accident in the Fukushima Daiichi nuclear power plant. Due to this accident, derived of the loss of the reactor cooling system, as well as of the prolonged absence of alternating and direct current, radiological protection actions were realized without being able to avoid the liberation of radioactive material to the atmosphere and ocean. The radiological impact of these liberations, not only in Japan but around the world, mainly in the north hemisphere of the Earth, was analyzed by means of environmental dose measurements and radionuclide concentrations in soil and water, among others. In the Mexico case, air samples data were obtained, as well as environmental dose celerity and full-length counts of the people coming from Japan near the disaster area. The present work contains the obtained results of the realized measurements in Mexico, same that have been used to make a summary and analysis of the dispersion in the environment in several countries of the world. (Author)

  5. Matrin 3 binds and stabilizes mRNA.

    Directory of Open Access Journals (Sweden)

    Maayan Salton

    Full Text Available Matrin 3 (MATR3 is a highly conserved, inner nuclear matrix protein with two zinc finger domains and two RNA recognition motifs (RRM, whose function is largely unknown. Recently we found MATR3 to be phosphorylated by the protein kinase ATM, which activates the cellular response to double strand breaks in the DNA. Here, we show that MATR3 interacts in an RNA-dependent manner with several proteins with established roles in RNA processing, and maintains its interaction with RNA via its RRM2 domain. Deep sequencing of the bound RNA (RIP-seq identified several small noncoding RNA species. Using microarray analysis to explore MATR3's role in transcription, we identified 77 transcripts whose amounts depended on the presence of MATR3. We validated this finding with nine transcripts which were also bound to the MATR3 complex. Finally, we demonstrated the importance of MATR3 for maintaining the stability of several of these mRNA species and conclude that it has a role in mRNA stabilization. The data suggest that the cellular level of MATR3, known to be highly regulated, modulates the stability of a group of gene transcripts.

  6. Oestradiol reduces Liver Receptor Homolog-1 mRNA transcript stability in breast cancer cell lines

    International Nuclear Information System (INIS)

    Lazarus, Kyren A.; Zhao, Zhe; Knower, Kevin C.; To, Sarah Q.; Chand, Ashwini L.; Clyne, Colin D.

    2013-01-01

    Highlights: •LRH-1 is an orphan nuclear receptor that regulates tumor proliferation. •In breast cancer, high mRNA expression is associated with ER+ status. •In ER−ve cells, despite very low mRNA, we found abundant LRH-1 protein. •Our data show distinctly different LRH-1 protein isoforms in ER− and ER+ breast cancer cells. •This is due to differences in LRH-1 mRNA and protein stability rates. -- Abstract: The expression of orphan nuclear receptor Liver Receptor Homolog-1 (LRH-1) is elevated in breast cancer and promotes proliferation, migration and invasion in vitro. LRH-1 expression is regulated by oestrogen (E 2 ), with LRH-1 mRNA transcript levels higher in oestrogen receptor α (ERα) positive (ER+) breast cancer cells compared to ER− cells. However, the presence of LRH-1 protein in ER− cells suggests discordance between mRNA transcript levels and protein expression. To understand this, we investigated the impact of mRNA and protein stability in determining LRH-1 protein levels in breast cancer cells. LRH-1 transcript levels were significantly higher in ER+ versus ER− breast cancer cells lines; however LRH-1 protein was expressed at similar levels. We found LRH-1 mRNA and protein was more stable in ER− compared to ER+ cell lines. The tumor-specific LRH-1 variant isoform, LRH-1v4, which is highly responsive to E 2 , showed increased mRNA stability in ER− versus ER+ cells. In addition, in MCF-7 and T47-D cell lines, LRH-1 total mRNA stability was reduced with E 2 treatment, this effect mediated by ERα. Our data demonstrates that in ER− cells, increased mRNA and protein stability contribute to the abundant protein expression levels. Expression and immunolocalisation of LRH-1 in ER− cells as well as ER− tumors suggests a possible role in the development of ER− tumors. The modulation of LRH-1 bioactivity may therefore be beneficial as a treatment option in both ER− and ER+ breast cancer

  7. Oestradiol reduces Liver Receptor Homolog-1 mRNA transcript stability in breast cancer cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Lazarus, Kyren A. [Cancer Drug Discovery Laboratory, Prince Henry’s Institute of Medical Research, Clayton, Victoria 3168 (Australia); Environmental and Biotechnology Centre, Swinburne University, Hawthorn, Victoria 3122 (Australia); Zhao, Zhe; Knower, Kevin C. [Cancer Drug Discovery Laboratory, Prince Henry’s Institute of Medical Research, Clayton, Victoria 3168 (Australia); To, Sarah Q. [Cancer Drug Discovery Laboratory, Prince Henry’s Institute of Medical Research, Clayton, Victoria 3168 (Australia); Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3168 (Australia); Chand, Ashwini L. [Cancer Drug Discovery Laboratory, Prince Henry’s Institute of Medical Research, Clayton, Victoria 3168 (Australia); Clyne, Colin D., E-mail: Colin.clyne@princehenrys.org [Cancer Drug Discovery Laboratory, Prince Henry’s Institute of Medical Research, Clayton, Victoria 3168 (Australia); Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3168 (Australia)

    2013-08-30

    Highlights: •LRH-1 is an orphan nuclear receptor that regulates tumor proliferation. •In breast cancer, high mRNA expression is associated with ER+ status. •In ER−ve cells, despite very low mRNA, we found abundant LRH-1 protein. •Our data show distinctly different LRH-1 protein isoforms in ER− and ER+ breast cancer cells. •This is due to differences in LRH-1 mRNA and protein stability rates. -- Abstract: The expression of orphan nuclear receptor Liver Receptor Homolog-1 (LRH-1) is elevated in breast cancer and promotes proliferation, migration and invasion in vitro. LRH-1 expression is regulated by oestrogen (E{sub 2}), with LRH-1 mRNA transcript levels higher in oestrogen receptor α (ERα) positive (ER+) breast cancer cells compared to ER− cells. However, the presence of LRH-1 protein in ER− cells suggests discordance between mRNA transcript levels and protein expression. To understand this, we investigated the impact of mRNA and protein stability in determining LRH-1 protein levels in breast cancer cells. LRH-1 transcript levels were significantly higher in ER+ versus ER− breast cancer cells lines; however LRH-1 protein was expressed at similar levels. We found LRH-1 mRNA and protein was more stable in ER− compared to ER+ cell lines. The tumor-specific LRH-1 variant isoform, LRH-1v4, which is highly responsive to E{sub 2}, showed increased mRNA stability in ER− versus ER+ cells. In addition, in MCF-7 and T47-D cell lines, LRH-1 total mRNA stability was reduced with E{sub 2} treatment, this effect mediated by ERα. Our data demonstrates that in ER− cells, increased mRNA and protein stability contribute to the abundant protein expression levels. Expression and immunolocalisation of LRH-1 in ER− cells as well as ER− tumors suggests a possible role in the development of ER− tumors. The modulation of LRH-1 bioactivity may therefore be beneficial as a treatment option in both ER− and ER+ breast cancer.

  8. Epidemiology, public health, and health surveillance around point sources of pollution

    International Nuclear Information System (INIS)

    Stebbings, J.H. Jr.

    1981-01-01

    In industrial society a large number of point sources of pollution exist, such as chemical plants, smelters, and nuclear power plants. Public concern has forced the practising epidemiologist to undertake health surveillance of the usually small populations living around point sources. Although not justifiable as research, such epidemiologic surveillance activities are becoming a routine part of public health practice, and this trend will continue. This introduction reviews concepts of epidemiologic surveillance, and institutional problems relating to the quality of such applied research

  9. Corrosion surveillance in spent fuel storage pools

    International Nuclear Information System (INIS)

    Howell, J.P.

    1996-01-01

    In mid-1991, corrosion of aluminum-clad spent nuclear fuel was observed in the light-water filled basins at the Savannah River site. A corrosion surveillance program was initiated in the P, K, L-Reactor basins and in the Receiving Basin for Offsite Fuels (RBOF). This program verified the aggressive nature of the pitting corrosion and provided recommendations for changes in basin operations to permit extended longer term interim storage. The changes were implemented during 1994--1996 and have resulted in significantly improved basin water quality with conductivity in the 1--3 microS/cm range. Under these improved conditions, no new pitting has been observed over the last three years. This paper describes the corrosion surveillance program at SRS and what has been learned about the corrosion of aluminum-clad in spent fuel storage pools

  10. The decapping activator Edc3 and the Q/N-rich domain of Lsm4 function together to enhance mRNA stability and alter mRNA decay pathway dependence in Saccharomyces cerevisiae

    Directory of Open Access Journals (Sweden)

    Susanne Huch

    2016-10-01

    Full Text Available The rate and regulation of mRNA decay are major elements in the proper control of gene expression. Edc3 and Lsm4 are two decapping activator proteins that have previously been shown to function in the assembly of RNA granules termed P bodies. Here, we show that deletion of edc3, when combined with a removal of the glutamine/asparagine rich region of Lsm4 (edc3Δ lsm4ΔC reduces mRNA stability and alters pathways of mRNA degradation. Multiple tested mRNAs exhibited reduced stability in the edc3Δ lsm4ΔC mutant. The destabilization was linked to an increased dependence on Ccr4-mediated deadenylation and mRNA decapping. Unlike characterized mutations in decapping factors that either are neutral or are able to stabilize mRNA, the combined edc3Δ lsm4ΔC mutant reduced mRNA stability. We characterized the growth and activity of the major mRNA decay systems and translation in double mutant and wild-type yeast. In the edc3Δ lsm4ΔC mutant, we observed alterations in the levels of specific mRNA decay factors as well as nuclear accumulation of the catalytic subunit of the decapping enzyme Dcp2. Hence, we suggest that the effects on mRNA stability in the edc3Δ lsm4ΔC mutant may originate from mRNA decay protein abundance or changes in mRNPs, or alternatively may imply a role for P bodies in mRNA stabilization.

  11. The analysis of reactor vessel surveillance program data

    International Nuclear Information System (INIS)

    Norris, E.B.

    1979-01-01

    Commercial nuclear power reactor vessel surveillance programs are provided by the reactor supplier and are designed to meet the requirements of ASTM Method E 185. (3). Each surveillance capsule contains sets of Charpy V-notch (Csub(v)) specimens representing selected materials from the vessel beltline region and some reference steel, tension test specimens machined from selected beltline materials, temperature monitors, and neutron flux dosimeters. Surveillance capsules may also contain fracture mechanics specimens machined from selected vessel beltline materials. The major steps in the conduct of a surveillance program include (1) the testing of the surveillance specimens to determine the exposure conditions at the capsule location and the resulting embrittlement of the vessel steel, (2) the extrapolation of the capsule results to the pressure vessel wall, and (3) the determination of the heatup and cooldown limits for normal, upset, and test operation. This paper will present data obtained from commercial light water reactor surveillance programs to illustrate the methods of analysis currently in use at Southwest Research Institute and to demonstrate some of the limitations imposed by the data available. Details concerning the procedures for testing the surveillance capsule specimens will not be included because they are considered to be outside of the scope of this paper

  12. Extended surveillance as a support to PLIM

    International Nuclear Information System (INIS)

    Walle, Eric van

    2002-01-01

    Full text: The safe exploitation of the reactor pressure vessel was and is always a major concern in nuclear power plant life management. At present, issues like Plant Life Extension, where utilities look into the possibility of license renewal after 40 years of operation, are becoming relevant in the USA. In other countries PLIM beyond the design life of the NPP could also be desirable from the economic viewpoint. The limiting factor could, however, be the integrity of the reactor pressure vessel. The reactor pressure vessel surveillance procedures as defined by regulatory legislation is limited and can be supplemented with valuable information that can be extracted in parallel to conventional surveillance testing or through additional testing on surveillance material. This is justified for several reasons: 1. The current methodology is semi-empirical, contains flaws and is in a number of cases over conservative. Without giving in on safety, we need to try and understand the material behavior more fundamentally; 2. Some reactor surveillance materials demonstrate inconsistent behavior with respect to the overall trend. These materials are called 'outlier' materials. But are they really outliers or is this connected to the indexing methodology used? 3. Additional data, for example the results of instrumented Charpy-V impact tests, have been obtained on many surveillance test specimens and are not adequately exploited in the actual surveillance methodology; 4. Scientific research provides substantial information and understanding of degradation mechanisms in reactor pressure vessel steels. Although we will not concentrate on this topic, the development of powerful microscopic investigation techniques, like FEGSTEM, APFIM, SANS, positron annihilation, internal friction, ... led to an intensified development of radiation damage modelling and are an input to micromechanical modelling. Moreover, due to the ever increasing computer power, additional multi-scale (time and

  13. Instrumentation of a Charpy-pendulum. Additional data obtained from it and its application to nuclear reactor pressure vessels surveillance programs; Instrumentacion de un pendulo Charpy. Datos adicionales obtenidos a partir de la misma y su aplicacion a programas de vigilancia de centrales nucleares

    Energy Technology Data Exchange (ETDEWEB)

    Chomik, Enrique P; Dhers, Horacio; Iorio, Antonio F [Comision Nacional de Energia Atomica, General San Martin (Argentina). Dept. de Materiales; Ciriani, Dario F [Comision Nacional de Energia Atomica, General San Martin (Argentina). Dept. de Combustibles Nucleares

    1999-07-01

    Charpy test gives information about a material dynamic fracture behavior. In a plain Charpy test, this information is the absorbed energy during fracture of the specimen, lateral deformation and the percentage of ductile fracture of the specimen. These parameters can then be used for the determination of the material response to a dynamic applied load, and are used at present to determine the brittle-ductile transition temperature of a material. However, there is a lot of additional information that can be obtained from a Charpy test, which is vital for the case of surveillance programs of nuclear power plants, where it is necessary to get the most available information from the specimens to be tested, because each one of them was irradiated for many years under temperature and neutronic flux conditions similar to that of the internal surface of the reactor pressure vessel, which converts these specimens in unique and very expensive ones. This additional information can be obtained from the curve that determines the evolution of the applied force to the specimen throughout the time involved in its fracture. It was possible to instrument a Charpy pendulum at a fraction of the cost necessary to buy an instrumentation package like the ones available in the market, and since the instrumentation equipment obtained is easy to transport. It has the additional advantage that can be used to instrument any other pendulum replacing only the hammer of the pendulum with a instrumented one for that pendulum. (author)

  14. Surface-water surveillance

    Energy Technology Data Exchange (ETDEWEB)

    Saldi, K.A.; Dirkes, R.L.; Blanton, M.L.

    1995-06-01

    This section of the 1994 Hanford Site Environmental Report summarizes the Surface water on and near the Hanford Site is monitored to determine the potential effects of Hanford operations. Surface water at Hanford includes the Columbia River, riverbank springs, ponds located on the Hanford Site, and offsite water systems directly east and across the Columbia River from the Hanford Site, and offsite water systems directly east and across the Columbia River from the Hanford Site. Columbia River sediments are also included in this discussion. Tables 5.3.1 and 5.3.2 summarize the sampling locations, sample types, sampling frequencies, and sample analyses included in surface-water surveillance activities during 1994. Sample locations are also identified in Figure 5.3.1. This section describes the surveillance effort and summarizes the results for these aquatic environments. Detailed analytical results are reported by Bisping (1995).

  15. Water radiological surveillance (II)

    International Nuclear Information System (INIS)

    Pablo San Martin de, M.

    2008-01-01

    This paper summarizes the characteristics of the Environmental Surveillance Radiological Networks (ESRN) currently operating in CEDEX. In the first part, the Spanish Continental Waters ESRN has been presented. This second one describes Spanish Costal Waters ESRN and the High Sensitivity Networks in Continental and Marine Waters. It also presents the Radiological Surveillance of Drinking Waters that CEDEX carries out in waters of public consumption management by the Canal de Isabel II (CYII) and by the Mancomunity of Canals Taibilla (M.C.T.). The legislation applicable in each case is reviewed as well. Due to its extension the article has been divided into two parts. As Spanish Continental Waters ESRN has been reviewed in the first part, the others ESRN are discussed in this second one. (Author) 10 refs

  16. Surface-water surveillance

    International Nuclear Information System (INIS)

    Saldi, K.A.; Dirkes, R.L.; Blanton, M.L.

    1995-01-01

    This section of the 1994 Hanford Site Environmental Report summarizes the Surface water on and near the Hanford Site is monitored to determine the potential effects of Hanford operations. Surface water at Hanford includes the Columbia River, riverbank springs, ponds located on the Hanford Site, and offsite water systems directly east and across the Columbia River from the Hanford Site, and offsite water systems directly east and across the Columbia River from the Hanford Site. Columbia River sediments are also included in this discussion. Tables 5.3.1 and 5.3.2 summarize the sampling locations, sample types, sampling frequencies, and sample analyses included in surface-water surveillance activities during 1994. Sample locations are also identified in Figure 5.3.1. This section describes the surveillance effort and summarizes the results for these aquatic environments. Detailed analytical results are reported by Bisping (1995)

  17. Medical Surveillance Monthly Report

    Science.gov (United States)

    2016-12-01

    Illness Prevention and Sun Safety. “Sun Safety.” https:// phc.amedd.army.mil/ topics /discond/hipss/Pages/ SunSafety.aspx. Accessed on 7 December 2016. 22...febrile illness; however, after its wide- spread introduction into immunologically MSMR Vol. 23 No. 12 December 2016 Page 8 naïve populations, a...October 2016 (data as of 22 November 2016) MSMR’s Invitation to Readers Medical Surveillance Monthly Report (MSMR) invites readers to submit topics for

  18. Internet and Surveillance

    DEFF Research Database (Denmark)

    The Internet has been transformed in the past years from a system primarily oriented on information provision into a medium for communication and community-building. The notion of “Web 2.0”, social software, and social networking sites such as Facebook, Twitter and MySpace have emerged in this co......The Internet has been transformed in the past years from a system primarily oriented on information provision into a medium for communication and community-building. The notion of “Web 2.0”, social software, and social networking sites such as Facebook, Twitter and MySpace have emerged...... institutions have a growing interest in accessing this personal data. Here, contributors explore this changing landscape by addressing topics such as commercial data collection by advertising, consumer sites and interactive media; self-disclosure in the social web; surveillance of file-sharers; privacy...... in the age of the internet; civil watch-surveillance on social networking sites; and networked interactive surveillance in transnational space. This book is a result of a research action launched by the intergovernmental network COST (European Cooperation in Science and Technology)....

  19. History of trichinellosis surveillance

    Directory of Open Access Journals (Sweden)

    Blancou J.

    2001-06-01

    Full Text Available The origin of trichinellosis, which existed in ancient times as testified by the discovery of parasite larvae on an Egyptian mummy, unfolded in several stages: discovery of encapsulated larvae (in the 1820s, identification and scientific description of these larvae (Paget Owen, 1835, followed by experimental infestations of animals (dogs, pigs, rabbits, mice or of humans as from 1850.The main occurrences of trichinellosis were followed with particular attention in Europe (Germany, Denmark, France, etc. and in the United States of America at the end of the XIXth century. They affected numerous domestic animal species (pigs, horses, etc. or wildlife and humans. Germany paid the heaviest toll with regard to the disease in humans, between 1860 and 1880, with several thousands of patients and more than 500 deaths.Different trichinellosis surveillance systems were set up in the relevant countries in the 1860s. In humans, this surveillance was carried out on affected living patients by a biopsy of the biceps muscles and subsequently by an analysis of eosinophilia (1895. In animals, surveillance was for a long time solely based on postmortem examination of the muscles of the affected animals. This method was used for the first time in 863 in Germany, and from the 1 890s, on several hundreds of thousands of pigs in Europe or in the United States of America.

  20. Nuclear safety research

    International Nuclear Information System (INIS)

    1999-01-01

    The NNSA checked and coordinated in 1999 the research project of the Surveillance Technology on Nuclear Installations under the National 9th-Five-Year Program to promote the organizations that undertake the research work on schedule and lay a foundation of obtaining achievements and effectiveness for the 9th-five-year plan on nuclear safety research

  1. SR proteins are NXF1 adaptors that link alternative RNA processing to mRNA export.

    Science.gov (United States)

    Müller-McNicoll, Michaela; Botti, Valentina; de Jesus Domingues, Antonio M; Brandl, Holger; Schwich, Oliver D; Steiner, Michaela C; Curk, Tomaz; Poser, Ina; Zarnack, Kathi; Neugebauer, Karla M

    2016-03-01

    Nuclear export factor 1 (NXF1) exports mRNA to the cytoplasm after recruitment to mRNA by specific adaptor proteins. How and why cells use numerous different export adaptors is poorly understood. Here we critically evaluate members of the SR protein family (SRSF1-7) for their potential to act as NXF1 adaptors that couple pre-mRNA processing to mRNA export. Consistent with this proposal, >1000 endogenous mRNAs required individual SR proteins for nuclear export in vivo. To address the mechanism, transcriptome-wide RNA-binding profiles of NXF1 and SRSF1-7 were determined in parallel by individual-nucleotide-resolution UV cross-linking and immunoprecipitation (iCLIP). Quantitative comparisons of RNA-binding sites showed that NXF1 and SR proteins bind mRNA targets at adjacent sites, indicative of cobinding. SRSF3 emerged as the most potent NXF1 adaptor, conferring sequence specificity to RNA binding by NXF1 in last exons. Interestingly, SRSF3 and SRSF7 were shown to bind different sites in last exons and regulate 3' untranslated region length in an opposing manner. Both SRSF3 and SRSF7 promoted NXF1 recruitment to mRNA. Thus, SRSF3 and SRSF7 couple alternative splicing and polyadenylation to NXF1-mediated mRNA export, thereby controlling the cytoplasmic abundance of transcripts with alternative 3' ends. © 2016 Müller-McNicoll et al.; Published by Cold Spring Harbor Laboratory Press.

  2. Tamper indicating radiation surveillance instrumentation

    International Nuclear Information System (INIS)

    Chambers, W.H.; Ney, J.F.

    1975-01-01

    Prototype personnel and shipping dock portal monitors suitable for unattended use were fabricated and tested. The requirement for continuous operation with only periodic inspection along with a desire for minimum costs and minimum interference with normal plant operation imposed unique design constraints. The design, operation, and performance of the detection and data recording instrumentation are described, as well as the tamper indicating techniques required to protect the collected data. The essential elements of either of the two instruments include a gamma detector array, signal conditioning electronics, digital alarm logic circuitry, power supplies, a microwave occupancy monitor, surveillance camera, irreversible electromechanical counters, and the appropriate tamper indicating envelope protecting these elements. Attempts to penetrate the tamper indicating envelope require material removal, and undetectable repair is very difficult, if not impossible. The techniques for joining major subassemblies and providing unique seals are also described. The personnel doorway uses a double pole array of NaI(Tl) detectors, and outputs are taken from a single channel pulse height analyzer with a window set at 60 to 250 keV and the lower level discriminator at greater than 60 keV. A sliding interval counter is used to make comparisons to an accumulated background at the 4sigma level. Logic design, sensitivity for special nuclear materials, false alarm data, and test procedures are described in detail. The shipping dock monitor had different design constraints and therefore uses a single, long, cylindrical plastic scintillator. Some differences in signal conditioning and processing are also described. (auth)

  3. Consequences of metaphase II oocyte cryopreservation on mRNA content.

    Science.gov (United States)

    Chamayou, S; Bonaventura, G; Alecci, C; Tibullo, D; Di Raimondo, F; Guglielmino, A; Barcellona, M L

    2011-04-01

    We studied the consequences of freezing/thawing processes on mRNA contents in MII oocytes after slow-freezing/rapid thawing (SF/RT) and vitrification/warming (V/W) protocols, and compared the results to fresh MII oocytes. We quantified the nuclear transcript mRNA responsible for the translation of proteins belonging either to trans-regulatory protein family or to functional structural proteins such as proteins involved in DNA structural organization (NAP1L1, TOP1, H1F0H1), chromosomal structure maintenance (SMC, SCC3, RAD21, SMC1A, SMC1B, STAG3, REC8), mitochondrial energetic pathways (ATP5GJ, SDHC), cell cycle regulation and processes (CLTA, MAPK6, CKS2) and staminal cell potency-development competence stage (DPPA3, OCT4, FOXJ2). Surplus MII oocytes were donated from patients in IVF cycles and divided in three groups of 15 oocytes. Group 1 was comprised of non-cryopreserved oocytes and Groups 2 and 3 underwent SF/RT and V/W procedures, respectively. There was an overall decrease of mRNA extracted from cryopreserved oocytes compared to control group. Only 39.4% of mRNA content were preserved after SF/RT while 63.3% of mRNA content were maintained after V/W. Oocyte cryopreservation is associated with molecular injury associated with the decrease of stored mRNA. However the V/W protocol is more conservative than SF/RT resulting in a level of mRNA sufficient to maintain biologic functions in the subsequent fertilized oocyte. Copyright © 2011 Elsevier Inc. All rights reserved.

  4. The RDE-10/RDE-11 complex triggers RNAi-induced mRNA degradation by association with target mRNA in C. elegans.

    Science.gov (United States)

    Yang, Huan; Zhang, Ying; Vallandingham, Jim; Li, Hua; Li, Hau; Florens, Laurence; Mak, Ho Yi

    2012-04-15

    The molecular mechanisms for target mRNA degradation in Caenorhabditis elegans undergoing RNAi are not fully understood. Using a combination of genetic, proteomic, and biochemical approaches, we report a divergent RDE-10/RDE-11 complex that is required for RNAi in C. elegans. Genetic analysis indicates that the RDE-10/RDE-11 complex acts in parallel to nuclear RNAi. Association of the complex with target mRNA is dependent on RDE-1 but not RRF-1, suggesting that target mRNA recognition depends on primary but not secondary siRNA. Furthermore, RDE-11 is required for mRNA degradation subsequent to target engagement. Deep sequencing reveals a fivefold decrease in secondary siRNA abundance in rde-10 and rde-11 mutant animals, while primary siRNA and microRNA biogenesis is normal. Therefore, the RDE-10/RDE-11 complex is critical for amplifying the exogenous RNAi response. Our work uncovers an essential output of the RNAi pathway in C. elegans.

  5. Wallops Ship Surveillance System

    Science.gov (United States)

    Smith, Donna C.

    2011-01-01

    Approved as a Wallops control center backup system, the Wallops Ship Surveillance Software is a day-of-launch risk analysis tool for spaceport activities. The system calculates impact probabilities and displays ship locations relative to boundary lines. It enables rapid analysis of possible flight paths to preclude the need to cancel launches and allow execution of launches in a timely manner. Its design is based on low-cost, large-customer- base elements including personal computers, the Windows operating system, C/C++ object-oriented software, and network interfaces. In conformance with the NASA software safety standard, the system is designed to ensure that it does not falsely report a safe-for-launch condition. To improve the current ship surveillance method, the system is designed to prevent delay of launch under a safe-for-launch condition. A single workstation is designated the controller of the official ship information and the official risk analysis. Copies of this information are shared with other networked workstations. The program design is divided into five subsystems areas: 1. Communication Link -- threads that control the networking of workstations; 2. Contact List -- a thread that controls a list of protected item (ocean vessel) information; 3. Hazard List -- threads that control a list of hazardous item (debris) information and associated risk calculation information; 4. Display -- threads that control operator inputs and screen display outputs; and 5. Archive -- a thread that controls archive file read and write access. Currently, most of the hazard list thread and parts of other threads are being reused as part of a new ship surveillance system, under the SureTrak project.

  6. Environmental surveillance master sampling schedule

    Energy Technology Data Exchange (ETDEWEB)

    Bisping, L.E.

    1993-01-01

    Environmental surveillance of the Hanford Site and surrounding areas is conducted by the Pacific Northwest Laboratory (PNL) for the US Department of Energy (DOE). Samples are routinely collected and analyzed to determine the quality of air, surface water, ground water, soil, sediment, wildlife, vegetation, foodstuffs, and farm products at Hanford Site and surrounding communities. This document contains the planned schedule for routine sample collection for the Surface Environmental Surveillance Project (SESP) and Drinking Water Project, and Ground-Water Surveillance Project.

  7. Self-surveillance

    DEFF Research Database (Denmark)

    Albrechtslund, Anders

    Gadgets and applications are increasingly being developed and used for tracking, quantifying, and documenting everyday life activities and especially health and fitness devices such as GPS-enabled sports watches are well-known and popular. However, self-surveillance practices involving networked...... pressure, fitness activities, sleep cycles, etc. can be broadcasted, e.g. as tweets on Twitter or status updates on Facebook. Such quantification practices with monitoring technologies become co-producing when individuals constitute themselves as subjects engaging in self-tracking, self-care, and self...

  8. Surveillance test interval optimization

    International Nuclear Information System (INIS)

    Cepin, M.; Mavko, B.

    1995-01-01

    Technical specifications have been developed on the bases of deterministic analyses, engineering judgment, and expert opinion. This paper introduces our risk-based approach to surveillance test interval (STI) optimization. This approach consists of three main levels. The first level is the component level, which serves as a rough estimation of the optimal STI and can be calculated analytically by a differentiating equation for mean unavailability. The second and third levels give more representative results. They take into account the results of probabilistic risk assessment (PRA) calculated by a personal computer (PC) based code and are based on system unavailability at the system level and on core damage frequency at the plant level

  9. GSFC Supplier Surveillance

    Science.gov (United States)

    Kelly, Michael P.

    2011-01-01

    Topics covered include: Develop Program/Project Quality Assurance Surveillance Plans The work activities performed by the developer and/or his suppliers are subject to evaluation and audit by government-designated representatives. CSO supports project by selecting on-site supplier representative s by one of several methods: (1) a Defense Contract Management Agency (DCMA) person via a Letter Of Delegation (LOD), (2) an independent assurance contractor (IAC) via a contract Audits, Assessments, and Assurance (A3) Contract Code 300 Mission Assurance Support Contract (MASC)

  10. An Interaction between KSHV ORF57 and UIF Provides mRNA-Adaptor Redundancy in Herpesvirus Intronless mRNA Export

    Science.gov (United States)

    Jackson, Brian R.; Boyne, James R.; Noerenberg, Marko; Taylor, Adam; Hautbergue, Guillaume M.; Walsh, Matthew J.; Wheat, Rachel; Blackbourn, David J.; Wilson, Stuart A.; Whitehouse, Adrian

    2011-01-01

    The hTREX complex mediates cellular bulk mRNA nuclear export by recruiting the nuclear export factor, TAP, via a direct interaction with the export adaptor, Aly. Intriguingly however, depletion of Aly only leads to a modest reduction in cellular mRNA nuclear export, suggesting the existence of additional mRNA nuclear export adaptor proteins. In order to efficiently export Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs from the nucleus, the KSHV ORF57 protein recruits hTREX onto viral intronless mRNAs allowing access to the TAP-mediated export pathway. Similarly however, depletion of Aly only leads to a modest reduction in the nuclear export of KSHV intronless mRNAs. Herein, we identify a novel interaction between ORF57 and the cellular protein, UIF. We provide the first evidence that the ORF57-UIF interaction enables the recruitment of hTREX and TAP to KSHV intronless mRNAs in Aly-depleted cells. Strikingly, depletion of both Aly and UIF inhibits the formation of an ORF57-mediated nuclear export competent ribonucleoprotein particle and consequently prevents ORF57-mediated mRNA nuclear export and KSHV protein production. Importantly, these findings highlight that redundancy exists in the eukaryotic system for certain hTREX components involved in the mRNA nuclear export of intronless KSHV mRNAs. PMID:21814512

  11. Surface Environmental Surveillance Procedures Manual

    International Nuclear Information System (INIS)

    Hanf, Robert W.; Poston, Ted M.

    2000-01-01

    Shows and explains certain procedures needed for surface environmental surveillance. Hanford Site environmental surveillance is conducted by the Pacific Northwest National Laboratory (PNNL) for the U.S. Department of Energy (DOE) under the Surface Environmental Surveillance Project (SESP). The basic requirements for site surveillance are set fourth in DOE Order 5400.1, General Environmental Protection Program Requirements. Guidance for the SESP is provided in DOE Order 5484.1, Environmental Protection, Safety, and Health Protection Information Reporting Requirements and DOE Order 5400.5, Radiation Protection of the Public and Environment. Guidelines for environmental surveillance activities are provided in DOE/EH-0173T, Environmental Regulatory Guide for Radiological Effluent Monitoring and Environmental Surveillance. An environmental monitoring plan for the Hanford Site is outlined in DOE/RL 91-50 Rev. 2, Environmental Monitoring Plan, United States Department of Energy, Richland Operations Office. Environmental surveillance data are used in assessing the impact of current and past site operations on human health and the environment, demonstrating compliance with applicable local, state, and federal environmental regulations, and verifying the adequacy of containment and effluent controls. SESP sampling schedules are reviewed, revised, and published each calendar year in the Hanford Site Environmental Surveillance Master Sampling Schedule. Environmental samples are collected by SESP staff in accordance with the approved sample collection procedures documented in this manual. Personnel training requirements are documented in SESP-TP-01 Rev.2, Surface Environmental Surveillance Project Training Program.

  12. Extension of responsibilities of the State Office for Nuclear Safety

    International Nuclear Information System (INIS)

    Hrehor, M.

    1995-01-01

    The responsibilities of the State Office for Nuclear Safety have been extended by Act No. 85/1995 to cover protection against ionizing radiation. The following responsibilities of the State Office for Nuclear Safety are defined by the Act: a) state surveillance over nuclear safety of nuclear facilities, and over radioactive waste and spent fuel management; b) state surveillance over nuclear materials, their record-keeping and accountancy; c) state surveillance over the safeguarding of nuclear facilities and nuclear materials; d) state surveillance over selected materials, facilities and technologies used in the nuclear field, as well as dual-purpose materials and facilities; e) state surveillance over protection against ionizing radiation; f) coordination of the performance of the Radiation Monitoring Network over the Czech Republic and responsibility for international exchange of data on the radiological situation. The Act is reproduced in full, and the organizational structure of the Office is shown in a chart. (J.B.)

  13. Sonoma Persistent Surveillance System

    Energy Technology Data Exchange (ETDEWEB)

    Pennington, D M

    2006-03-24

    Sonoma offers the first cost-effective, broad-area, high-resolution, real-time motion imagery system for surveillance applications. Sonoma is unique in its ability to provide continuous, real-time video imagery of an area the size of a small city with resolutions sufficient to track 8,000 moving objects in the field of view. At higher resolutions and over smaller areas, Sonoma can even track the movement of individual people. The visual impact of the data available from Sonoma is already causing a paradigm shift in the architecture and operation of other surveillance systems. Sonoma is expected to cost just one-tenth the price of comparably sized sensor systems. Cameras mounted on an airborne platform constantly monitor an area, feeding data to the ground for real-time analysis. Sonoma was designed to provide real-time data for actionable intelligence in situations such as monitoring traffic, special events, border security, and harbors. If a Sonoma system had been available in the aftermath of the Katrina and Rita hurricanes, emergency responders would have had real-time information on roads, water levels, and traffic conditions, perhaps saving many lives.

  14. Development of a sealing process of capsules for surveillance test tubes of the vessel in nuclear power plants; Desarrollo de proceso de sellado de capsulas para probetas de vigilancia de la vasija en nucleoelectricas

    Energy Technology Data Exchange (ETDEWEB)

    Romero C, J.; Fernandez T, F.; Perez R, N.; Rocamontes A, M.; Garcia R, R. [ININ, Km 36.5 Carretera Mexico-Toluca, 52750 La Marquesa, Estado de Mexico (Mexico)

    2007-07-01

    The surveillance capsule is composed by the support, three capsules for impact test tubes, five capsules for tension test tubes and one porta dosemeters. The capsules for test tubes are of two types: rectangular capsule for Charpy test tubes and cylindrical capsule for tension test tubes. This work describes the development of the welding system to seal the capsules for test tubes that should contain helium of ultra high purity to a pressure of 1 atmosphere. (Author)

  15. International conference on strengthening of nuclear safety in Eastern Europe. Armenian Nuclear Regulatory Authority

    International Nuclear Information System (INIS)

    Nersesyan, V.

    1999-01-01

    The status of the Armenian Nuclear Regulatory Authority (ANRA) are described in detail with its main task and responsibilities concerning regulations and surveillance of nuclear and radiation safety. The following issues are presented: nuclear legislation; inspection activities; licensing of significant safety related modifications and modernization of NPPs; incidents at NPPs; personnel training; emergency planning; surveillance of nuclear materials; radioactive waste management; and plan of the ANRA perspective development

  16. Authentication Approaches for Standoff Video Surveillance

    International Nuclear Information System (INIS)

    Baldwin, G.; Sweatt, W.; Thomas, M.

    2015-01-01

    Video surveillance for international nuclear safeguards applications requires authentication, which confirms to an inspector reviewing the surveillance images that both the source and the integrity of those images can be trusted. To date, all such authentication approaches originate at the camera. Camera authentication would not suffice for a ''standoff video'' application, where the surveillance camera views an image piped to it from a distant objective lens. Standoff video might be desired in situations where it does not make sense to expose sensitive and costly camera electronics to contamination, radiation, water immersion, or other adverse environments typical of hot cells, reprocessing facilities, and within spent fuel pools, for example. In this paper, we offer optical architectures that introduce a standoff distance of several metres between the scene and camera. Several schemes enable one to authenticate not only that the extended optical path is secure, but also that the scene is being viewed live. They employ optical components with remotely-operated spectral, temporal, directional, and intensity properties that are under the control of the inspector. If permitted by the facility operator, illuminators, reflectors and polarizers placed in the scene offer further possibilities. Any tampering that would insert an alternative image source for the camera, although undetectable with conventional cryptographic authentication of digital camera data, is easily exposed using the approaches we describe. Sandia National Laboratories is a multi-programme laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000. Support to Sandia National Laboratories provided by the NNSA Next Generation Safeguards Initiative is gratefully acknowledged. SAND2014-3196 A. (author)

  17. 2012 Sexually Transmitted Diseases Surveillance

    Science.gov (United States)

    ... Data Appendix Tables A1 - A4 STD Surveillance Case Definitions Contributors Related Links STD Home STD Data & Statistics NCHHSTP Atlas Interactive STD Data - 1996-2013 STD Health Equity HIV/AIDS Surveillance & Statistics Follow STD STD on Twitter STD on Facebook File Formats Help: How do I view different ...

  18. Surveillance - filling the gap between audits and inspection

    International Nuclear Information System (INIS)

    Coulombe, C.T.

    1987-01-01

    Historically, two major verification activities are accomplished at nuclear plants: audits and inspections. Both have their roots firmly planted in regulation. They are required elements of a quality assurance (QA) program. Inspection, focused on hardware, verifies that equipment meets its specified requirements. Auditing, focused on documentation, verifies, through objective evidence, that the QA program is being effectively implemented. Quality surveillance, focused on performance, verifies effective use of the plant's procedures and quality program. The surveillance concept provides a method to assure that the gap between the inspection function and the audit function is filled in

  19. Principles of mRNA transport in yeast.

    Science.gov (United States)

    Heym, Roland Gerhard; Niessing, Dierk

    2012-06-01

    mRNA localization and localized translation is a common mechanism by which cellular asymmetry is achieved. In higher eukaryotes the mRNA transport machinery is required for such diverse processes as stem cell division and neuronal plasticity. Because mRNA localization in metazoans is highly complex, studies at the molecular level have proven to be cumbersome. However, active mRNA transport has also been reported in fungi including Saccharomyces cerevisiae, Ustilago maydis and Candida albicans, in which these events are less difficult to study. Amongst them, budding yeast S. cerevisiae has yielded mechanistic insights that exceed our understanding of other mRNA localization events to date. In contrast to most reviews, we refrain here from summarizing mRNA localization events from different organisms. Instead we give an in-depth account of ASH1 mRNA localization in budding yeast. This approach is particularly suited to providing a more holistic view of the interconnection between the individual steps of mRNA localization, from transcriptional events to cytoplasmic mRNA transport and localized translation. Because of our advanced mechanistic understanding of mRNA localization in yeast, the present review may also be informative for scientists working, for example, on mRNA localization in embryogenesis or in neurons.

  20. The surveillance error grid.

    Science.gov (United States)

    Klonoff, David C; Lias, Courtney; Vigersky, Robert; Clarke, William; Parkes, Joan Lee; Sacks, David B; Kirkman, M Sue; Kovatchev, Boris

    2014-07-01

    Currently used error grids for assessing clinical accuracy of blood glucose monitors are based on out-of-date medical practices. Error grids have not been widely embraced by regulatory agencies for clearance of monitors, but this type of tool could be useful for surveillance of the performance of cleared products. Diabetes Technology Society together with representatives from the Food and Drug Administration, the American Diabetes Association, the Endocrine Society, and the Association for the Advancement of Medical Instrumentation, and representatives of academia, industry, and government, have developed a new error grid, called the surveillance error grid (SEG) as a tool to assess the degree of clinical risk from inaccurate blood glucose (BG) monitors. A total of 206 diabetes clinicians were surveyed about the clinical risk of errors of measured BG levels by a monitor. The impact of such errors on 4 patient scenarios was surveyed. Each monitor/reference data pair was scored and color-coded on a graph per its average risk rating. Using modeled data representative of the accuracy of contemporary meters, the relationships between clinical risk and monitor error were calculated for the Clarke error grid (CEG), Parkes error grid (PEG), and SEG. SEG action boundaries were consistent across scenarios, regardless of whether the patient was type 1 or type 2 or using insulin or not. No significant differences were noted between responses of adult/pediatric or 4 types of clinicians. Although small specific differences in risk boundaries between US and non-US clinicians were noted, the panel felt they did not justify separate grids for these 2 types of clinicians. The data points of the SEG were classified in 15 zones according to their assigned level of risk, which allowed for comparisons with the classic CEG and PEG. Modeled glucose monitor data with realistic self-monitoring of blood glucose errors derived from meter testing experiments plotted on the SEG when compared to

  1. Smart sensing surveillance system

    Science.gov (United States)

    Hsu, Charles; Chu, Kai-Dee; O'Looney, James; Blake, Michael; Rutar, Colleen

    2010-04-01

    An effective public safety sensor system for heavily-populated applications requires sophisticated and geographically-distributed infrastructures, centralized supervision, and deployment of large-scale security and surveillance networks. Artificial intelligence in sensor systems is a critical design to raise awareness levels, improve the performance of the system and adapt to a changing scenario and environment. In this paper, a highly-distributed, fault-tolerant, and energy-efficient Smart Sensing Surveillance System (S4) is presented to efficiently provide a 24/7 and all weather security operation in crowded environments or restricted areas. Technically, the S4 consists of a number of distributed sensor nodes integrated with specific passive sensors to rapidly collect, process, and disseminate heterogeneous sensor data from near omni-directions. These distributed sensor nodes can cooperatively work to send immediate security information when new objects appear. When the new objects are detected, the S4 will smartly select the available node with a Pan- Tilt- Zoom- (PTZ) Electro-Optics EO/IR camera to track the objects and capture associated imagery. The S4 provides applicable advanced on-board digital image processing capabilities to detect and track the specific objects. The imaging detection operations include unattended object detection, human feature and behavior detection, and configurable alert triggers, etc. Other imaging processes can be updated to meet specific requirements and operations. In the S4, all the sensor nodes are connected with a robust, reconfigurable, LPI/LPD (Low Probability of Intercept/ Low Probability of Detect) wireless mesh network using Ultra-wide band (UWB) RF technology. This UWB RF technology can provide an ad-hoc, secure mesh network and capability to relay network information, communicate and pass situational awareness and messages. The Service Oriented Architecture of S4 enables remote applications to interact with the S4

  2. Smart sensing surveillance system

    Science.gov (United States)

    Hsu, Charles; Chu, Kai-Dee; O'Looney, James; Blake, Michael; Rutar, Colleen

    2010-04-01

    Unattended ground sensor (UGS) networks have been widely used in remote battlefield and other tactical applications over the last few decades due to the advances of the digital signal processing. The UGS network can be applied in a variety of areas including border surveillance, special force operations, perimeter and building protection, target acquisition, situational awareness, and force protection. In this paper, a highly-distributed, fault-tolerant, and energyefficient Smart Sensing Surveillance System (S4) is presented to efficiently provide 24/7 and all weather security operation in a situation management environment. The S4 is composed of a number of distributed nodes to collect, process, and disseminate heterogeneous sensor data. Nearly all S4 nodes have passive sensors to provide rapid omnidirectional detection. In addition, Pan- Tilt- Zoom- (PTZ) Electro-Optics EO/IR cameras are integrated to selected nodes to track the objects and capture associated imagery. These S4 camera-connected nodes will provide applicable advanced on-board digital image processing capabilities to detect and track the specific objects. The imaging detection operations include unattended object detection, human feature and behavior detection, and configurable alert triggers, etc. In the S4, all the nodes are connected with a robust, reconfigurable, LPI/LPD (Low Probability of Intercept/ Low Probability of Detect) wireless mesh network using Ultra-wide band (UWB) RF technology, which can provide an ad-hoc, secure mesh network and capability to relay network information, communicate and pass situational awareness and messages. The S4 utilizes a Service Oriented Architecture such that remote applications can interact with the S4 network and use the specific presentation methods. The S4 capabilities and technologies have great potential for both military and civilian applications, enabling highly effective security support tools for improving surveillance activities in densely crowded

  3. [Entomological surveillance in Mauritius].

    Science.gov (United States)

    Gopaul, R

    1995-01-01

    The entomological surveillance is an essential link in the fight against malaria in Mauritius. Because of the large number of malaria-infected travellers in Mauritius and the presence of the vector Anopheles arabiensis, the risk of local transmission is very real. The medical entomology division together with the malaria control unit and the health appointees exert a rigorous entomological surveillance of malaria. Field agents make entomological investigations of pilot villages and around the harbor and airport, where there have been cases of malaria, in addition to a few randomly chosen regions. All of the inhabited regions are accessible because of a good highway infrastructure, which enables a complete coverage for the entomological prospectives. Entomological controls are also conducted in the airplanes and the ships. All of the captured mosquitos and the harvested larva are transferred to a laboratory for identification, dissection or sensibility tests, etc. The larva of A. arabiensis have not yet developed resistance to Temephos and the adults are still sensitive to DDT. Thus, the larval habitats are treated with Temephos and DDT is sprayed in the residences where there have been native cases of malaria. The entomology division studies the ecology and the evolution of the larval habitats, as well as the impact of the anti-larval fight on the anophelene density. In addition to the chemical fight, a biological control is being tried with larva-eating fish such as Lebistes and Tilapia. In general, the anophelene density in Mauritius is low, but after the big summer rains, especially during a period of cyclones, there is a considerable increase of larval habitats and consequently a higher number of A. arabiensis. Therefore during this season, it is necessary to make an even more rigorous entomological surveillance. A. arabiensis has a strong exophile tendency even if it is endophage and exophage. This mosquito is zoophile, mostly towards cattle, and the

  4. Surveillance theory and its implications for law

    NARCIS (Netherlands)

    Timan, Tjerk; Galic, Masa; Koops, Bert-Jaap; Brownsword, Roger; Scotford, Eloise; Yeung, Karen

    2017-01-01

    This chapter provides an overview of key surveillance theories and their implications for law and regulation. It presents three stages of theories that characterise changes in thinking about surveillance in society and the disciplining, controlling, and entertaining functions of surveillance.

  5. Reporting and Surveillance for Norovirus Outbreaks

    Science.gov (United States)

    ... Vaccine Surveillance Network (NVSN) Foodborne Diseases Active Surveillance Network (FoodNet) National Outbreak Reporting System (NORS) Estimates of Foodborne Illness in the United States CDC's Vessel Sanitation Program CDC Feature: Surveillance for Norovirus Outbreaks Top ...

  6. Nuclear Export of Messenger RNA

    Directory of Open Access Journals (Sweden)

    Jun Katahira

    2015-03-01

    Full Text Available Transport of messenger RNA (mRNA from the nucleus to the cytoplasm is an essential step of eukaryotic gene expression. In the cell nucleus, a precursor mRNA undergoes a series of processing steps, including capping at the 5' ends, splicing and cleavage/polyadenylation at the 3' ends. During this process, the mRNA associates with a wide variety of proteins, forming a messenger ribonucleoprotein (mRNP particle. Association with factors involved in nuclear export also occurs during transcription and processing, and thus nuclear export is fully integrated into mRNA maturation. The coupling between mRNA maturation and nuclear export is an important mechanism for providing only fully functional and competent mRNA to the cytoplasmic translational machinery, thereby ensuring accuracy and swiftness of gene expression. This review describes the molecular mechanism of nuclear mRNA export mediated by the principal transport factors, including Tap-p15 and the TREX complex.

  7. Nuclear Export of Messenger RNA

    Science.gov (United States)

    Katahira, Jun

    2015-01-01

    Transport of messenger RNA (mRNA) from the nucleus to the cytoplasm is an essential step of eukaryotic gene expression. In the cell nucleus, a precursor mRNA undergoes a series of processing steps, including capping at the 5' ends, splicing and cleavage/polyadenylation at the 3' ends. During this process, the mRNA associates with a wide variety of proteins, forming a messenger ribonucleoprotein (mRNP) particle. Association with factors involved in nuclear export also occurs during transcription and processing, and thus nuclear export is fully integrated into mRNA maturation. The coupling between mRNA maturation and nuclear export is an important mechanism for providing only fully functional and competent mRNA to the cytoplasmic translational machinery, thereby ensuring accuracy and swiftness of gene expression. This review describes the molecular mechanism of nuclear mRNA export mediated by the principal transport factors, including Tap-p15 and the TREX complex. PMID:25836925

  8. An overview of environmental surveillance of waste management activities at the Idaho National Engineering Laboratory

    Science.gov (United States)

    Smith, T.H.; Chew, E.W.; Hedahl, T.G.; Mann, L.J.; Pointer, T.F.; Wiersma, G.B.

    1986-01-01

    The Idaho National Engineering Laboratory (INEL), in southeastern Idaho, is a principal center for nuclear energy development for the Department of Energy (DOE) and the U.S. Nuclear Navy. Fifty-two reactors have been built at the INEL, with 15 still operable. Extensive environmental surveillance is conducted at the INEL by DOE's Radiological Environmental Sciences Laboratory (RESL), and the U.S. Geological Survey (USGS), the National Oceanic and Atmospheric Administration (NOAA), EG&G Idaho, Inc., and Westinghouse Idaho Nuclear Company (WINCO). Surveillance of waste management facilities radiation is integrated with the overall INEL Site surveillance program. Air, warer, soil, biota, and environmental radiation are monitored or sampled routinely at INEL. Results to date indicate very small or no impacts from INEL on the surrounding environment. Environmental surveillance activities are currently underway to address key environmental issues at the INEL.

  9. Overview of environmental surveillance of waste management activities at the Idaho National Engineering Laboratory

    International Nuclear Information System (INIS)

    Smith, T.H.; Hedahl, T.G.; Wiersma, G.B.; Chew, E.W.; Mann, L.J.; Pointer, T.F.

    1986-02-01

    The Idaho National Engineering Laboratory (INEL), in southeastern Idaho, is a principal center for nuclear energy development for the Department of Energy (DOE) and the US Nuclear Navy. Fifty-two reactors have been built at the INEL, with 15 still operable. Extensive environmental surveillance is conducted at the INEL by DOE's Radiological and Environmental Sciences Laboratory (RESL), the US Geological Survey (USGS), the National Oceanic and Atmospheric Administration (NOAA), EG and G Idaho, Inc., and Westinghouse Idaho Nuclear Company (WINCO). Surveillance of waste management facilities is integrated with the overall INEL Site surveillance program. Air, water, soil, biota, and environmental radiation are monitored or sampled routinely at the INEL. Results to date indicate very small or no impacts from the INEL on the surrounding environment. Environmental surveillance activities are currently underway to address key environmental issues at the INEL. 7 refs., 6 figs., 2 tabs

  10. Total process surveillance: (TOPS)

    International Nuclear Information System (INIS)

    Millar, J.H.P.

    1992-01-01

    A Total Process Surveillance system is under development which can provide, in real-time, additional process information from a limited number of raw measurement signals. This is achieved by using a robust model based observer to generate estimates of the process' internal states. The observer utilises the analytical reduncancy among a diverse range of transducers and can thus accommodate off-normal conditions which lead to transducer loss or damage. The modular hierarchical structure of the system enables the maximum amount of information to be assimilated from the available instrument signals no matter how diverse. This structure also constitutes a data reduction path thus reducing operator cognitive overload from a large number of varying, and possibly contradictory, raw plant signals. (orig.)

  11. Secure surveillance videotapes

    International Nuclear Information System (INIS)

    Resnik, W.M.; Kadner, S.P.; Olsen, R.; Chitumbo, K.; Pepper, S.

    1995-01-01

    With assistance from the US Program for Technical Assistance to IAEA Safeguards (POTAS), Aquila Technologies Group developed the Tamper-Resistant Analog Media (TRAM-1000) system to provide standard VHS surveillance video tapes with an enhanced tamper-indicating capability. This project represents further implementation of the partnership approach in facilities including light water reactors with MOX facilities. These facilities use Uniplex Digiquad system video tapes. The partnership approach ensures that one organization can exchange the tapes in a machine without the presence of the other, without losing continuity of information. The TRAM-1000 system development project was accomplished in two stages. In the first stage of the project, the original system delivered to the IAEA, consists of three parts: (1) the tamper detection unit, (2) a specially augmented VHS video tape, and (3) an HP-95 reader. The tamper detection unit houses a VACOSS active fiber-optic seal and an electronic identification tag (E-TAG) reader. In the second stage of the project, the original TRAM-1000 was modified to its current design based on agency input. After delivery of the original TRAM-1000 system to the IAEA, it was reviewed by inspectors. The inspectors felt that the initial system's tape storage/transport method could be simplified. Rather than threading the fiber through the tape spindles, the inspectors suggested that the tape be placed in a bag capable of being sealed. Also, a more flexible fiber-optic cable was recommended. As a result of these suggestions, Aquila developed a tamper-proof bag specifically for holding a surveillance video tape and sealable with a VACOSS fiber optical seal

  12. Nuclear facility safeguards as specified by the Czechoslovak administrative law

    International Nuclear Information System (INIS)

    Elias, J.; Svab, J.

    1978-01-01

    A study is presented of the legal aspects of nuclear safeguards for the operation of nuclear power facilities evaluating the development of the legal arrangement over the past five years, i.e., encoding nuclear safeguards for nuclear facilities in the new building regulations (Act No. 50/1976 Coll. of Laws on Urban Planning and Building Regulations and implementing provisions). It also discusses the juridical position of State surveillance over the nuclear safety of nuclear facilities and its relation to surveillance carried out by specialized bodies of the State work safety inspection and to surveillance carried out by hygiene inspection bodies. (J.S.)

  13. Optimization and studies of the welding processes, automation of the sealing welding system and fracture mechanics in the vessels surveillance in nuclear power plants; Optimizacion y estudios de los procesos de soldadura, automatizacion del sistema de soldadura de sellado y mecanica de fractura en la vigilancia de vasijas en nucleoelectricas

    Energy Technology Data Exchange (ETDEWEB)

    Gama R, G.

    2011-07-01

    Inside this work the optimization of two welding systems is described, as well as the conclusion of a system for the qualification of containers sealing in the National Institute of Nuclear Research that have application in the surveillance programs of nuclear reactors vessels and the correspondent extension of the operation license. The test tubes Charpy are assay to evaluate the embrittlement grade, when obtaining the increment in the reference temperature and the decrease of the absorbed maximum energy, in the transition curve fragile-ductile of the material. After the test two test tube halves are obtained that should take advantage to follow the surveillance of the vessel and their possible operation extension, this is achieved by means of rebuilding (being obtained of a tested test tube two reconstituted test tubes). The welding system for the rebuilding of test tubes Charpy, was optimized when diminishing the union force at solder, achieving the elimination of the rejection for penetration lack for spill. For this work temperature measurements were carried out at different distances of the welding interface from 1 up to 12 mm, obtaining temperature profiles. With the maximum temperatures were obtained a graph and equation that represents the maximum temperature regarding the distance of the interface, giving as a result practical the elimination of other temperature measurements. The reconstituted test tubes were introduced inside pressurized containers with helium of ultra high purity to 1 pressure atmosphere. This process was carried out in the welding system for containers sealing, where an automatic process was implemented by means of an application developed in the program LabVIEW, reducing operation times and allowing the remote control of the process, the acquisition parameters as well as the generation of welding reports, avoiding with this the human error. (Author)

  14. Surveillance of environmental radiation in Finland. Annual Report 2000

    International Nuclear Information System (INIS)

    Mustonen, R.

    2001-01-01

    The main goal of the surveillance of environmental radioactivity is to be always aware of levels of radiation to which the public is exposed. Another goal is to detect all remarkable changes in levels of environmental radiation and radioactivity. Compliance with the basic safety standards laid down for protection of the health of the general public against dangers arising from ionising radiation can be ensured with environmental radiation surveillance. Running of surveillance programmes on continuous basis also maintains and develops competence and readiness to respond to radiological emergencies. Surveillance of environmental radioactivity in Finland is one of the official obligations of the Radiation and Nuclear Safety Authority (STUK). This obligation is based on the national and the European Communities' legislation. The Finnish radiation protection legislation appoints STUK as the national authority responsible for surveillance of environmental radioactivity, and the Euratom Treaty assumes continuous monitoring of levels of radioactivity in the air, water and soil in the Member States. In Finland, also the Finnish Meteorological Institute (FMI) and the Defence Forces are monitoring environmental radiation at their own stations. This report summarises the results of environmental radiation surveillance in 2000. The report also contains some comparisons with results from the previous years. The results are collected from monitoring programmes of STUK, FMI and the Defence Forces Research Institute of Technology. Nuclear power plant licensees are responsible for environmental surveillance in the vicinity of nuclear power plants in Finland. Those results are reported elsewhere. STUK's partners in surveillance of environmental radioactivity are collecting and delivering samples for laboratory analyses, or are participating in whole-body counting. STUK would like to express its gratitude to the following institutions for the successful co-operation: Defence Forces

  15. Surveillance of environmental radiation in Finland. Annual Report 2002

    International Nuclear Information System (INIS)

    Mustonen, R.

    2003-01-01

    The main goal of the surveillance of environmental radioactivity is to be always aware of the levels of radiation to which the public is exposed. Another goal is to detect all remarkable changes in the levels of environmental radiation and radioactivity. Compliance with the basic safety standards laid down for protection of the general public against dangers arising from ionising radiation can be ensured with environmental radiation surveillance. Running of surveillance programmes on a continuous basis also maintains and develops competence and readiness to respond to radiological emergencies. Surveillance of environmental radioactivity in Finland is one of the official obligations of the Radiation and Nuclear Safety Authority (STUK). This obligation is based on both national and EU legislation. The Finnish radiation protection legislation appoints STUK as the national authority responsible for the surveillance of environmental radioactivity, and the Euratom Treaty assumes continuous monitoring of levels of radioactivity in the air, water and soil in the Member States. In Finland, the Finnish Meteorological Institute (FMI) and the Defence Forces also monitor environmental radiation at their own stations. This report summarises the results of environmental radiation surveillance in 2002. The report also contains some comparisons with results from the previous years. The results are obtained from the monitoring programmes of STUK, FMI and the Defence Forces Research Institute of Technology. Nuclear power plant licensees are responsible for environmental surveillance in the vicinity of nuclear power plants in Finland. These results are reported elsewhere. STUK's partners in the surveillance of environmental radioactivity collect and deliver environmental samples for laboratory analyses, or participate in whole-body counting. STUK would like to express its gratitude to the following institutions for successful co-operation: The Finnish Defence Forces, the Finnish

  16. Surveillance of environmental radiation in Finland. Annual Report 2001

    International Nuclear Information System (INIS)

    Mustonen, R.

    2002-01-01

    The main goal of the surveillance of environmental radioactivity is to be always aware of levels of radiation to which the public is exposed. Another goal is to detect all remarkable changes in levels of environmental radiation and radioactivity. Compliance with the basic safety standards laid down for protection of health of the general public against dangers arising from ionising radiation can be ensured with environmental radiation surveillance. Running of surveillance programmes on continuous basis also maintains and develops competence and readiness to respond to radiological emergencies. Surveillance of environmental radioactivity in Finland is one of the official obligations of the Radiation and Nuclear Safety Authority (STUK). This obligation is based on the national and the European Communities' legislation. The Finnish radiation protection legislation appoints STUK as the national authority responsible for surveillance of environmental radioactivity, and the Euratom Treaty assumes continuous monitoring of levels of radioactivity in the air, water and soil in the Member States. In Finland, also the Finnish Meteorological Institute (FMI) and the Defence Forces are monitoring environmental radiation at their own stations. This report summarises the results of environmental radiation surveillance in 2001. The report also contains some comparisons with results from the previous years. The results are collected from monitoring programmes of STUK, FMI and the Defence Forces Research Institute of Technology. Nuclear power plant licensees are responsible for environmental surveillance in the vicinity of nuclear power plants in Finland. Those results are reported elsewhere. STUK's partners in surveillance of environmental radioactivity are collecting and delivering environmental samples for laboratory analyses, or are participating in whole-body counting. STUK would like to express its gratitude to the following institutions for the successful co-operation: Defence

  17. Surveillance of environmental radiation in Finland. Annual Report 2003

    International Nuclear Information System (INIS)

    Mustonen, R.

    2004-01-01

    The main goal of the surveillance of environmental radioactivity is to be always aware of the levels of radiation to which the public is exposed. Another goal is to detect all remarkable changes in the levels of environmental radiation and radioactivity. Compliance with the basic safety standards laid down for protection of the general public against dangers arising from ionising radiation can be ensured with environmental radiation surveillance. Running of surveillance programmes on a continuous basis also maintains and develops competence and readiness to respond to radiological emergencies. Surveillance of environmental radioactivity in Finland is one of the official obligations of the Radiation and Nuclear Safety Authority (STUK). This obligation is based on both national and EU legislation. The Finnish radiation protection legislation appoints STUK as the national authority responsible for the surveillance of environmental radioactivity, and the Euratom Treaty assumes continuous monitoring of levels of radioactivity in the air, water and soil in the Member States. In Finland, the Finnish Meteorological Institute (FMI) and the Defence Forces also monitor environmental radiation at their own stations. This report summarises the results of environmental radiation surveillance in 2003. The report also contains some comparisons with results from the previous years. The results are obtained from the monitoring programmes of STUK, FMI and the Defence Forces Research Institute of Technology. Nuclear power plant licensees are responsible for environmental surveillance in the vicinity of nuclear power plants in Finland. These results are reported elsewhere. STUK's partners in the surveillance of environmental radioactivity collect and deliver environmental samples for laboratory analyses, or participate in whole-body counting. STUK would like to express its gratitude to the following institutions for successful co- operation: The Finnish Defence Forces, the Finnish

  18. Cytoplasmic Control of Sense-Antisense mRNA Pairs

    Directory of Open Access Journals (Sweden)

    Flore Sinturel

    2015-09-01

    Full Text Available Transcriptome analyses have revealed that convergent gene transcription can produce many 3′-overlapping mRNAs in diverse organisms. Few studies have examined the fate of 3′-complementary mRNAs in double-stranded RNA-dependent nuclear phenomena, and nothing is known about the cytoplasmic destiny of 3′-overlapping messengers or their impact on gene expression. Here, we demonstrate that the complementary tails of 3′-overlapping mRNAs can interact in the cytoplasm and promote post-transcriptional regulatory events including no-go decay (NGD in Saccharomyces cerevisiae. Genome-wide experiments confirm that these messenger-interacting mRNAs (mimRNAs form RNA duplexes in wild-type cells and thus have potential roles in modulating the mRNA levels of their convergent gene pattern under different growth conditions. We show that the post-transcriptional fate of hundreds of mimRNAs is controlled by Xrn1, revealing the extent to which this conserved 5′-3′ cytoplasmic exoribonuclease plays an unexpected but key role in the post-transcriptional control of convergent gene expression.

  19. Cytoplasmic Control of Sense-Antisense mRNA Pairs.

    Science.gov (United States)

    Sinturel, Flore; Navickas, Albertas; Wery, Maxime; Descrimes, Marc; Morillon, Antonin; Torchet, Claire; Benard, Lionel

    2015-09-22

    Transcriptome analyses have revealed that convergent gene transcription can produce many 3'-overlapping mRNAs in diverse organisms. Few studies have examined the fate of 3'-complementary mRNAs in double-stranded RNA-dependent nuclear phenomena, and nothing is known about the cytoplasmic destiny of 3'-overlapping messengers or their impact on gene expression. Here, we demonstrate that the complementary tails of 3'-overlapping mRNAs can interact in the cytoplasm and promote post-transcriptional regulatory events including no-go decay (NGD) in Saccharomyces cerevisiae. Genome-wide experiments confirm that these messenger-interacting mRNAs (mimRNAs) form RNA duplexes in wild-type cells and thus have potential roles in modulating the mRNA levels of their convergent gene pattern under different growth conditions. We show that the post-transcriptional fate of hundreds of mimRNAs is controlled by Xrn1, revealing the extent to which this conserved 5'-3' cytoplasmic exoribonuclease plays an unexpected but key role in the post-transcriptional control of convergent gene expression. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

  20. Expressed prostatic secretion biomarkers improve stratification of NCCN active surveillance candidates: performance of secretion capacity and TMPRSS2:ERG models.

    Science.gov (United States)

    Whelan, Christopher; Kawachi, Mark; Smith, David D; Linehan, Jennifer; Babilonia, Gail; Mejia, Rosa; Wilson, Timothy; Smith, Steven S

    2014-01-01

    Active surveillance is a viable patient option for prostate cancer provided that a clinical determination of low risk and presumably organ confined disease can be made. To standardize risk stratification schemes the NCCN (National Comprehensive Cancer Network®) provides guidelines for the active surveillance option. We determined the effectiveness of expressed prostatic secretion biomarkers for detecting occult risk factors in NCCN active surveillance candidates. Expressed prostatic secretion specimens were obtained before robot-assisted radical prostatectomy. Secretion capacity biomarkers, including total RNA and expressed prostatic secretion specimen volume, were measured by standard techniques. RNA expression biomarkers, including TXNRD1 mRNA, prostate specific antigen mRNA, TMPRSS2:ERG fusion mRNA and PCA3 mRNA, were measured by quantitative reverse-transcription polymerase chain reaction. Of the 528 patients from whom expressed prostatic secretions were collected 216 were eligible for active surveillance under NCCN guidelines. Variable selection on logistic regression identified 2 models, including one featuring types III and VI TMPRSS2:ERG variants, and one featuring 2 secretion capacity biomarkers. Of the 2 high performing models the secretion capacity model was most effective for detecting cases in this group that were up-staged or up-staged plus upgraded. It decreased the risk of up-staging in patients with a negative test almost eightfold and decreased the risk of up-staging plus upgrading about fivefold while doubling the prevalence of up-staging in the positive test group. Noninvasive expressed prostatic secretion testing may improve patient acceptance of active surveillance by dramatically reducing the presence of occult risk factors among those eligible for active surveillance under NCCN guidelines. Copyright © 2014 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

  1. mRNA Cancer Vaccines-Messages that Prevail.

    Science.gov (United States)

    Grunwitz, Christian; Kranz, Lena M

    2017-01-01

    During the last decade, mRNA became increasingly recognized as a versatile tool for the development of new innovative therapeutics. Especially for vaccine development, mRNA is of outstanding interest and numerous clinical trials have been initiated. Strikingly, all of these studies have proven that large-scale GMP production of mRNA is feasible and concordantly report a favorable safety profile of mRNA vaccines. Induction of T-cell immunity is a multi-faceted process comprising antigen acquisition, antigen processing and presentation, as well as immune stimulation. The effectiveness of mRNA vaccines is critically dependent on making the antigen(s) of interest available to professional antigen-presenting cells, especially DCs. Efficient delivery of mRNA into DCs in vivo remains a major challenge in the mRNA vaccine field. This review summarizes the principles of mRNA vaccines and highlights the importance of in vivo mRNA delivery and recent advances in harnessing their therapeutic potential.

  2. Radioisotopic Thermoelectric Generator (RTG) Surveillance

    Energy Technology Data Exchange (ETDEWEB)

    Mulford, Roberta Nancy [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-09-29

    This lecture discusses stockpile stewardship efforts and the role surveillance plays in the process. Performance of the RTGs is described, and the question of the absence of anticipated He is addressed.

  3. Youth Risk Behavior Surveillance System

    Science.gov (United States)

    ... Youth Risk Behavior Surveillance System (YRBSS) monitors six types of health-risk behaviors that contribute to the leading causes of death and disability among youth and adults, including— Behaviors that contribute ...

  4. Final report: FASEB Summer Research Conference on ''Post-transcriptional control of gene expression: Effectors of mRNA decay'' [agenda and attendees list

    Energy Technology Data Exchange (ETDEWEB)

    Maquat, Lynne

    2002-12-01

    The goal of this meeting was to provide an interactive forum for scientists working on prokaryotic and eukaryotic mRNA decay. A special seminar presented by a leader in the field of mRNA decay in S. cerevisiae focused on what is known and what needs to be determined, not only for yeast but for other organisms. The large attendance (110 participants) reflects the awareness that mRNA decay is a key player in gene regulation in a way that is affected by the many steps that precede mRNA formation. Sessions were held on the following topics: mRNA transport and mRNP; multicomponent eukaryotic nucleases; nonsense-mediated mRNA decay and nonsense-associated altered splicing; Cis-acting sequences/Trans-acting factors of mRNA decay; translational accuracy; multicomponent bacterial nucleases; interplay between mRNA polyadenylation, translation and decay in prokaryotes and prokaryotic organelles; and RNA interference and other RNA mediators of gene expression. In addition to the talks and two poster sessions, there were three round tables: (1) Does translation occur in the nucleus? (2) Differences and similarities in the mechanisms of mRNA decay in different eukaryotes, and (3) RNA surveillance in bacteria?

  5. Non-process instrumentation surveillance and test reduction

    International Nuclear Information System (INIS)

    Ferrell, R.; LeDonne, V.; Donat, T.; Thomson, I.; Sarlitto, M.

    1993-12-01

    Analysis of operating experience, instrument failure modes, and degraded instrument performance has led to a reduction in Technical Specification surveillance and test requirements for nuclear power plant process instrumentation. These changes have resulted in lower plant operations and maintenance (O ampersand M) labor costs. This report explores the possibility of realizing similar savings by reducing requirements for non-process instrumentation. The project team reviewed generic Technical Specifications for the four major US nuclear steam supply system (NSSS) vendors (Westinghouse, General Electric, Combustion Engineering, and Babcock ampersand Wilcox) to identify nonprocess instrumentation for which surveillance/test requirements could be reduced. The team surveyed 10 utilities to identify specific non-process instrumentation at their plants for which requirements could be reduced. The team evaluated utility analytic approaches used to justify changes in surveillance/test requirements for process equipment to determine their applicability to non-process instrumentation. The report presents a prioritized list of non-process instrumentation systems suitable for surveillance/test requirements reduction. The top three systems in the list are vibration monitors, leak detection monitors, and chemistry monitors. In general, most non-process instrumentation governed by Technical Specification requirements are candidates for requirements reduction. If statistical requirements are somewhat relaxed, the analytic approaches previously used to reduce requirements for process instrumentation can be applied to non-process instrumentation. The report identifies as viable the technical approaches developed and successfully used by Southern California Edison, Arizona Public Service, and Boston Edison

  6. CYP3A5 mRNA degradation by nonsense-mediated mRNA decay.

    Science.gov (United States)

    Busi, Florent; Cresteil, Thierry

    2005-09-01

    The total CYP3A5 mRNA level is significantly greater in carriers of the CYP3A5*1 allele than in CYP3A5*3 homozygotes. Most of the CYP3A5*3 mRNA includes an intronic sequence (exon 3B) containing premature termination codons (PTCs) between exons 3 and 4. Two models were used to investigate the degradation of CYP3A5 mRNA: a CYP3A5 minigene consisting of CYP3A5 exons and introns 3 to 6 transfected into MCF7 cells, and the endogenous CYP3A5 gene expressed in HepG2 cells. The 3'-untranslated region g.31611C>T mutation has no effect on CYP3A5 mRNA decay. Splice variants containing exon 3B were more unstable than wild-type (wt) CYP3A5 mRNA. Cycloheximide prevents the recognition of PTCs by ribosomes: in transfected MCF7 and HepG2 cells, cycloheximide slowed down the degradation of exon 3B-containing splice variants, suggesting the participation of nonsense-mediated decay (NMD). When PTCs were removed from pseudoexon 3B or when UPF1 small interfering RNA was used to impair the NMD mechanism, the decay of the splice variant was reduced, confirming the involvement of NMD in the degradation of CYP3A5 splice variants. Induction could represent a source of variability for CYP3A5 expression and could modify the proportion of splice variants. The extent of CYP3A5 induction was investigated after exposure to barbiturates or steroids: CYP3A4 was markedly induced in a pediatric population compared with untreated neonates. However, no effect could be detected in either the total CYP3A5 RNA, the proportion of splice variant RNA, or the protein level. Therefore, in these carriers, induction is unlikely to switch on the phenotypic CYP3A5 expression in carriers of CYP3A5*3/*3.

  7. Health surveillance - myth and reality

    International Nuclear Information System (INIS)

    Sharp, C.

    1998-01-01

    This paper discusses the principles, health benefit and cost-effectiveness of health surveillance in the occupational setting, which apply to exposure to ionising radiations in the same manner as to other hazards in the workplace. It highlights the techniques for undertaking health surveillance, discusses their relative advantages and disadvantages and illustrates these in relation to specific hazards. The responsibilities of the medical staff and of the worker are also discussed. (author)

  8. Surface Environmental Surveillance Procedures Manual

    Energy Technology Data Exchange (ETDEWEB)

    RW Hanf; TM Poston

    2000-09-20

    Environmental surveillance data are used in assessing the impact of current and past site operations on human health and the environment, demonstrating compliance with applicable local, state, and federal environmental regulations, and verifying the adequacy of containment and effluent controls. SESP sampling schedules are reviewed, revised, and published each calendar year in the Hanford Site Environmental Surveillance Master Sampling Schedule. Environmental samples are collected by SESP staff in accordance with the approved sample collection procedures documented in this manual.

  9. Privacy Implications of Surveillance Systems

    DEFF Research Database (Denmark)

    Thommesen, Jacob; Andersen, Henning Boje

    2009-01-01

    This paper presents a model for assessing the privacy „cost‟ of a surveillance system. Surveillance systems collect and provide personal information or observations of people by means of surveillance technologies such as databases, video or location tracking. Such systems can be designed for vari......This paper presents a model for assessing the privacy „cost‟ of a surveillance system. Surveillance systems collect and provide personal information or observations of people by means of surveillance technologies such as databases, video or location tracking. Such systems can be designed...... for various purposes, even as a service for those being observed, but in any case they will to some degree invade their privacy. The model provided here can indicate how invasive any particular system may be – and be used to compare the invasiveness of different systems. Applying a functional approach......, the model is established by first considering the social function of privacy in everyday life, which in turn lets us determine which different domains will be considered as private, and finally identify the different types of privacy invasion. This underlying model (function – domain – invasion) then serves...

  10. Elementary Surveillance (ELS) and Enhanced Surveillance (EHS) Validation via Mode S Secondary Radar Surveillance

    National Research Council Canada - National Science Library

    Grappel, Robert D; Harris, Garrett S; Kozar, Mark J; Wiken, Randall T

    2008-01-01

    ...) and Enhanced Surveillance (ERS) data link applications. The intended audience for this report is an engineering staff assigned the task of implementing a monitoring system used to determine ELS and EHS compliance...

  11. Conserved CPEs in the p53 3' untranslated region influence mRNA stability and protein synthesis

    DEFF Research Database (Denmark)

    Rosenstierne, Maiken W; Vinther, Jeppe; Mittler, Gerhard

    2008-01-01

    CaT skin and MCF-7 breast cancer cell lines were established. Quantitative PCR and an enzymatic assay were used to quantify the reporter mRNA and protein levels, respectively. Proteins binding to the CPEs were identified by RNA-immunoprecipitation (IP) and quantitative mass spectroscopy. RESULTS: The wild...... irradiation. Several proteins (including GAPDH, heterogeneous nuclear ribonucleoprotein (hnRNP) D and A/B) were identified from the MCF-7 cytoplasmic extracts that bound specifically to the CPEs. CONCLUSION: Two conserved CPEs in the p53 3'UTR regulate stability and translation of a reporter mRNA in non...

  12. Critical Surveillance Studies in the Information Society

    Directory of Open Access Journals (Sweden)

    Thomas Allmer

    2011-11-01

    Full Text Available The overall aim of this paper is to clarify how we can theorize and systemize economic surveillance. Surveillance studies scholars like David Lyon stress that economic surveillance such as monitoring consumers or the workplace are central aspects of surveillance societies. The approach that is advanced in this work recognizes the importance of the role of the economy in contemporary surveillance societies. The paper at hand constructs theoretically founded typologies in order to systemize the existing literature of surveillance studies and to analyze examples of surveillance. Therefore, it mainly is a theoretical approach combined with illustrative examples. This contribution contains a systematic discussion of the state of the art of surveillance and clarifies how different notions treat economic aspects of surveillance. In this work it is argued that the existing literature is insufficient for studying economic surveillance. In contrast, a typology of surveillance in the modern economy, which is based on foundations of a political economy approach, allows providing a systematic analysis of economic surveillance on the basis of current developments on the Internet. Finally, some political recommendations are drawn in order to overcome economic surveillance. This contribution can be fruitful for scholars who want to undertake a systematic analysis of surveillance in the modern economy and who want to study the field of surveillance critically.

  13. Alternative Polyadenylation and Nonsense-Mediated Decay Coordinately Regulate the Human HFE mRNA Levels

    Science.gov (United States)

    Martins, Rute; Proença, Daniela; Silva, Bruno; Barbosa, Cristina; Silva, Ana Luísa; Faustino, Paula; Romão, Luísa

    2012-01-01

    Nonsense-mediated decay (NMD) is an mRNA surveillance pathway that selectively recognizes and degrades defective mRNAs carrying premature translation-termination codons. However, several studies have shown that NMD also targets physiological transcripts that encode full-length proteins, modulating their expression. Indeed, some features of physiological mRNAs can render them NMD-sensitive. Human HFE is a MHC class I protein mainly expressed in the liver that, when mutated, can cause hereditary hemochromatosis, a common genetic disorder of iron metabolism. The HFE gene structure comprises seven exons; although the sixth exon is 1056 base pairs (bp) long, only the first 41 bp encode for amino acids. Thus, the remaining downstream 1015 bp sequence corresponds to the HFE 3′ untranslated region (UTR), along with exon seven. Therefore, this 3′ UTR encompasses an exon/exon junction, a feature that can make the corresponding physiological transcript NMD-sensitive. Here, we demonstrate that in UPF1-depleted or in cycloheximide-treated HeLa and HepG2 cells the HFE transcripts are clearly upregulated, meaning that the physiological HFE mRNA is in fact an NMD-target. This role of NMD in controlling the HFE expression levels was further confirmed in HeLa cells transiently expressing the HFE human gene. Besides, we show, by 3′-RACE analysis in several human tissues that HFE mRNA expression results from alternative cleavage and polyadenylation at four different sites – two were previously described and two are novel polyadenylation sites: one located at exon six, which confers NMD-resistance to the corresponding transcripts, and another located at exon seven. In addition, we show that the amount of HFE mRNA isoforms resulting from cleavage and polyadenylation at exon seven, although present in both cell lines, is higher in HepG2 cells. These results reveal that NMD and alternative polyadenylation may act coordinately to control HFE mRNA levels, possibly varying its

  14. The upstream open reading frame of cyclin-dependent kinase inhibitor 1A mRNA negatively regulates translation of the downstream main open reading frame

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Kyoung Mi; Cho, Hana [School of Life Sciences and Biotechnology, Korea University, Seoul 136-701 (Korea, Republic of); Kim, Yoon Ki, E-mail: yk-kim@korea.ac.kr [School of Life Sciences and Biotechnology, Korea University, Seoul 136-701 (Korea, Republic of)

    2012-08-03

    Highlights: Black-Right-Pointing-Pointer CDKN1A mRNA is a bona fide NMD substrate. Black-Right-Pointing-Pointer The uORF of CDKN1A mRNA is efficiently translated. Black-Right-Pointing-Pointer Translation of downstream main ORF is negatively regulated by translation of uORF in CDKN1A mRNA. -- Abstract: The first round of translation occurs on mRNAs bound by nuclear cap-binding complex (CBC), which is composed of nuclear cap-binding protein 80 and 20 (CBP80/20). During this round of translation, aberrant mRNAs are recognized and downregulated in abundance by nonsense-mediated mRNA decay (NMD), which is one of the mRNA quality control mechanisms. Here, our microarray analysis reveals that the level of cyclin-dependent kinase inhibitor 1A (CDKN1A; also known as Waf1/p21) mRNAs increases in cells depleted of cellular NMD factors. Intriguingly, CDKN1A mRNA contains an upstream open reading frame (uORF), which is a NMD-inducing feature. Using chimeric reporter constructs, we find that the uORF of CDKN1A mRNA negatively modulates translation of the main downstream ORF. These findings provide biological insights into the possible role of NMD in diverse biological pathways mediated by CDKN1A.

  15. Increased IL-10 mRNA and IL-23 mRNA expression in multiple sclerosis

    DEFF Research Database (Denmark)

    Krakauer, Martin; Sorensen, P; Khademi, M

    2008-01-01

    BACKGROUND: Interferon (IFN)-beta therapy in multiple sclerosis (MS) has been suggested to promote a deviation from T lymphocyte production of pathogenic Th1 cytokines to less detrimental Th2 cytokines, but this is still controversial. We studied patterns of in vivo blood mononuclear cell (MNC...... of any Th1 or Th2 cytokines. The largest changes in cytokine mRNA levels occurred early (~9-12 h) after an IFN-beta injection. CONCLUSION: We found no evidence of a Th1- or Th2-mRNA-promoting effect of IFN-beta therapy. The therapeutic effect of IFN-beta is more likely attributable to the induction...

  16. Surveillance of environmental radiation in Finland. Annual Report 2004

    International Nuclear Information System (INIS)

    Mustonen, R.

    2005-07-01

    The main goal of the surveillance of environmental radioactivity is to be always aware of levels of artificial radiation in the environment to which the public is exposed. Another goal is to detect all remarkable changes in levels of environmental radiation and radioactivity. Compliance with the basic safety standards laid down for protection of health of the general public against dangers arising from ionising radiation can be ensured with environmental radiation surveillance. Running of surveillance programmes on continuous basis also maintains and develops competence and readiness to respond to radiological emergencies. Surveillance of environmental radiation contains surveillance of artificial radiation and artificial radioactive elements in the environment. Natural radiation and natural radioactive elements are not associated with the surveillance programme, although the greater part of the public exposure to radiation is caused by natural radiation. Exposure to natural radiation is controlled separately if there is reason to suspect that natural radioactive elements cause unusual high exposure to the public (e.g. indoor radon and natural radionuclides in drinking water). Surveillance of environmental radioactivity in Finland is one of the official obligations of the Radiation and Nuclear Safety Authority (STUK). This obligation is based on the national and the European Communities' legislation. The Finnish radiation protection legislation appoints STUK as the national authority responsible for surveillance of environmental radioactivity, and the Euratom Treaty assumes continuous monitoring of levels of radioactivity in the air, water and soil in the Member States. In Finland, also the Finnish Meteorological Institute (FMI) and the Defence Forces are monitoring environmental radiation at their own stations. This report summarises the results of environmental radiation surveillance in 2004. The report also contains some comparisons with results from the

  17. Surveillance of environmental radiation in Finland. Annual report 2011; Ympaeristoen saeteilyvalvonta Suomessa. Vuosiraportti 2011

    Energy Technology Data Exchange (ETDEWEB)

    Mustonen, R. (ed.)

    2012-08-15

    The main goal of the surveillance of environmental radioactivity is to be always aware of levels of artificial radiation in the environment to which the public is exposed. Another goal is to detect all remarkable changes in levels of environmental radiation and radioactivity. Compliance with the basic safety standards laid down for protection of health of the general public against dangers arising from ionising radiation can be ensured with environmental radiation surveillance. Running of surveillance programmes on continuous basis also maintains and develops competence and readiness to respond to radiological emergencies. This report summarises the results of environmental radiation surveillance in 2011. The report also contains some comparisons with results from the previous years. Surveillance of environmental radiation contains surveillance of artificial radiation and artificial radioactive elements in the environment. Natural radiation and natural radioactive elements are not associated with the surveillance programme, although the greater part of the public exposure to radiation is caused by natural radiation. Exposure to natural radiation is controlled separately if there is reason to suspect, that natural radioactive elements cause unusual high exposure to the public (e.g. indoor radon and natural radionuclides in drinking water). Nuclear power plant licensees are responsible for environmental surveillance in the vicinity of nuclear power plants in Finland. Those results are reported elsewhere. Surveillance of environmental radioactivity in Finland is one of the official obligations of the Radiation and Nuclear Safety Authority (STUK). This obligation is based on the national and the European Communities' legislation. The Finnish radiation protection legislation appoints STUK as the national authority responsible for surveillance of environmental radioactivity, and the Euratom Treaty assumes continuous monitoring of levels of radioactivity in the air

  18. Project Surveillance and Maintenance Plan

    International Nuclear Information System (INIS)

    1985-09-01

    The Project Surveillance and Maintenance Plan (PSMP) describes the procedures that will be used by the US Department of Energy (DOE), or other agency as designated by the President to verify that inactive uranium tailings disposal facilities remain in compliance with licensing requirements and US Environmental Protection Agency (EPA) standards for remedial actions. The PSMP will be used as a guide for the development of individual Site Surveillance and Maintenance Plans (part of a license application) for each of the UMTRA Project sites. The PSMP is not intended to provide minimum requirements but rather to provide guidance in the selection of surveillance measures. For example, the plan acknowledges that ground-water monitoring may or may not be required and provides the [guidance] to make this decision. The Site Surveillance and Maintenance Plans (SSMPs) will form the basis for the licensing of the long-term surveillance and maintenance of each UMTRA Project site by the NRC. Therefore, the PSMP is a key milestone in the licensing process of all UMTRA Project sites. The Project Licensing Plan (DOE, 1984a) describes the licensing process. 11 refs., 22 figs., 8 tabs

  19. State of the reactor vessel surveillance programs in Korea and foreign countries

    International Nuclear Information System (INIS)

    Kim, Jeong Kyu; Hwang, Jong Keun; Park, Keon Woo; Kim, Bum Sik; Jeong, Kyung Hoon

    1996-06-01

    ASTM standards are dominating all over the world in the field of the reactor vessel surveillance program. They are mainly used directly or that the national standards in use correspond quite well with ASTM. According to, however, increasing concerns about the protection of environment and safety of nuclear plant, various approaches to establish and reinforce the national standards are made actively in Europe. In addition, some methods to share the nuclear data by integrating the existing test, analysis procedures and units system are considered. For nuclear plants in Korea, MOST Notice No. 92-20 should be applied for all PWRs after UCN units 3 and 4 since it was promulgated at Dec. 1992. The notice almost reflects the contents of ASTM E 185. But, the notice has much to be desired to provide the technical back-ground for reactor vessel surveillance program because it is not a standard such as ASTM or ASME code but regulation such as CFR or RG. Several Korean Standards are also used in limited area of the surveillance program. Therefore, practical requirements and rules for surveillance program are in accordance with the ASTM and CFR. In this report, the state of application of the standards to the surveillance program in Korea and Europe are reviewed and their national standards re compared with US standards or regulations. Current level and the future prospect of surveillance technology for PWR vessel are discussed at this point of view. 15 tabs., 12 figs., 38 refs. (Author)

  20. State surveillance of radioactive material transportation. Final report

    International Nuclear Information System (INIS)

    Salomon, S.N.

    1984-02-01

    The main objective of this final report on the state surveillance of the transportation of radioactive material (RAM) is to suggest the most cost-effective inspection areas where enforcement actions might be taken by the states during their participation in the State Hazardous Materials Enforcement Development (SHMED) Program. On the basis of the lessons learned from the surveillance program, these actions are enforcement at low-level radioactive burial sites by means of civil penalties and site use suspension; enforcement at airports and at terminals that forward freight; and enforcement of courier companies. More effective and efficient enforcement can be achieved through instrumented police patrol cars and remote surveillance because they require the least amount of time of enforcement personnel. In addition, there is a strong relationship between effective emergency response and enforcement because the appropriate shipping papers, placarding and knowledge of appropriate emergency response procedures lead to improved emergency response. These lessons originate from a ten-state surveillance program from 1977 through 1981 jointly sponsored by the US Nuclear Regulatory Commission (NRC) and DOT. The states give recommendations in the categories of education, training, expanded surveillance, coordination and enforcement. The topics of special interest covered include low-level radioactive waste disposal sites, airports, cargo terminals, highways, ports, and accidents and incidents. The three most common problems in compliance with RAM transportation regulations reported by the states are incorrect package labeling; improper shipping papers; and incorrect or missing placards. Other common problems reported by the states are summarized. The relationship to other studies, the status of the SHMED Program, a synopsis of state RAM surveillance reports, and NRC/DOT expenditures are given

  1. Studies on the role of NonA in mRNA biogenesis

    International Nuclear Information System (INIS)

    Kozlova, Natalia; Braga, Jose; Lundgren, Josefin; Rino, Jose; Young, Patrick; Carmo-Fonseca, Maria; Visa, Neus

    2006-01-01

    The NonA protein of Drosophila melanogaster is an abundant nuclear protein that belongs to the DBHS (Drosophila behavior, human splicing) protein family. The DBHS proteins bind both DNA and RNA in vitro and have been involved in different aspects of gene expression, including pre-mRNA splicing, transcription regulation and nuclear retention of mRNA. We have used double-stranded RNA interference in Drosophila S2 cells to silence the expression of NonA and to investigate its role in mRNA biogenesis. We show that knockdown of NonA does not affect transcription nor splicing. We demonstrate that NonA forms a complex with the essential nuclear export factor NXF1 in an RNA-dependent manner. We have constructed stable S2 cell lines that express full-length and truncated NXF1 fused to GFP in order to perform fluorescence recovery after photobleaching experiments. We show that knockdown of NonA reduces the intranuclear mobility of NXF1-GFP associated with poly(A) + RNA in vivo, while the mobility of the truncated NXF1-GFP that does not bind RNA is not affected. Our data suggest that NonA facilitates the intranuclear mobility of mRNP particles

  2. Informatics enables public health surveillance

    Directory of Open Access Journals (Sweden)

    Scott J. N McNabb

    2017-01-01

    Full Text Available Over the past decade, the world has radically changed. New advances in information and communication technologies (ICT connect the world in ways never imagined. Public health informatics (PHI leveraged for public health surveillance (PHS, can enable, enhance, and empower essential PHS functions (i.e., detection, reporting, confirmation, analyses, feedback, response. However, the tail doesn't wag the dog; as such, ICT cannot (should not drive public health surveillance strengthening. Rather, ICT can serve PHS to more effectively empower core functions. In this review, we explore promising ICT trends for prevention, detection, and response, laboratory reporting, push notification, analytics, predictive surveillance, and using new data sources, while recognizing that it is the people, politics, and policies that most challenge progress for implementation of solutions.

  3. Health surveillance of radiological work

    International Nuclear Information System (INIS)

    Pauw, H.; Vliet, J.V.D.; Zuidema, H.

    1988-01-01

    Shielding x-ray devices and issuing film badges to radiological workers in 1936 can be considered the start of radiological protection in the Philips enterprises in the Netherlands. Shielding and equipment were constantly improved based upon the dosimetry results of the filmbadges. The problem of radioactive waste led to the foundation of a central Philips committee for radiological protection in 1956, which in 1960 also issued an internal license system in order to regulate the proper precautions to be taken : workplace design and layout, technological provisions and working procedures. An evaluation of all radiological work in 1971 learnt that a stricter health surveillance program was needed to follow up the precautions issued by the license. On one hand a health surveillance program was established and on the other hand all types of radiological work were classified. In this way an obligatory and optimal health surveillance program was issued for each type of radiological work

  4. Harrisburg highlights need for nuclear stethoscope

    International Nuclear Information System (INIS)

    Anon.

    1982-01-01

    A team at the University of New South Wales has almost completed developing a data acquisition system for nuclear plant surveillance. It consists of a computer interfaced with a digital correlator, a Fourier transform unit and multi-channel analyser

  5. Harrisburg highlights need for nuclear stethoscope

    Energy Technology Data Exchange (ETDEWEB)

    1982-12-01

    A team at the University of New South Wales has almost completed developing a data acquisition system for nuclear plant surveillance. It consists of a computer interfaced with a digital correlator, a Fourier transform unit and multi-channel analyser.

  6. Containment and Surveillance Equipment Compendium

    International Nuclear Information System (INIS)

    Luetters, F.O.

    1980-02-01

    The Containment and Surveillance Equipment Compendium contains information sections describing the application and status of seals, optical surveillance systems, and monitors for international safeguards systems. The Compendium is a collection of information on equipment in use (generally by the IAEA) or under development in the US in diverse programs being conducted at numerous facilities under different sponsors. The Compendium establishes a baseline for the status and applications of C/S equipment and is a tool to assist in the planning of future C/S hardware development activities. The Appendix contains design concepts which can be developed to meet future goals

  7. Environmental monitoring around nuclear power plants of EDF in France; La surveillance de l'environnement autour des centres nucleaires de production d'electricite d'EDF en France

    Energy Technology Data Exchange (ETDEWEB)

    Chretien, V.; Hemidy, P.Y. [Electricite de France (EDF/DPN), 93 - Saint-Denis (France)

    2010-06-15

    As part of the regulatory environmental monitoring around its nuclear power plants, EDF carries out every year more than 40.000 measurements. In addition EDF performs more precise radioecological surveys on all its sites. This monitoring shows the minor incidence of EDF's nuclear power plants on the environment and a general decrease of gamma emitters radionuclides in the environment. These results confirm the efforts lead by EDF to minimise its impacts on the environment, linked to an efficient waste management and demonstrate the good operating standards of its plants. On a qualitative viewpoint, significant efforts on both metrology and organization have lead to improved measurements capabilities, in order to achieve NF EN ISO/CEI 17025 standard compliance. More recently, a similar approach has been implemented with sampling methods. While today the level achieved in the number and quality of measurement complies with the objectives of the monitoring of the impact of the nuclear power plants on the environment, the results of this monitoring should be presented to the public with more pedagogy to give an adequate answer to their expectations. The new questions that will undoubtedly be raised by the recent availability of this information on the Internet should reinforce this dialogue and should also be the opportunity to improve the quality of our communication. (author)

  8. A novel use of LIMS for surveillance activities at the Savannah River Site

    International Nuclear Information System (INIS)

    Rogier, W.J.

    2000-01-01

    The current mission of the Savannah River Site is focused primarily on cleanup and disposal of waste associated with more than 40 years of nuclear material production. However, SRS continues to provide tritium processing for the Department of Energy. Tritium, a radioactive isotope of hydrogen gas, is used to boost the explosive power of nuclear weapons. The tritium container, processed by SRS, is known as a reservoir. Part of the SRS tritium mission is to assure the safety and reliability of tritium reservoirs by conducting a series of thorough surveillance tests on a sampling of fielded reservoirs. Data from these tests have historically been stored in a database archive and reporting system known as QUADSTAR. This system was developed at the Mound Facility in the mid-1980s when Mound performed the reservoir surveillance mission for DOE. The surveillance mission and the QUADSTAR database were transferred to SRS during the downsizing of the Nuclear Weapon Complex in the mid-1990s

  9. Radiological assessment of the French environment in 2008. Synthesis of the IRSN's surveillance networks

    International Nuclear Information System (INIS)

    Chaptal-Gradoz, N.; Chevreuil, M.; D'amico, D.; Debayle, Ch.; Leprieur, F.; Manificat, G.; Peres, J.M.; Pierrard, O.; Tournieux, D.; Veran-Viguie, M.P.; Renaud, Ph.; Roussel-Debet, S.; Masson, O.; Pourcelot, L.; Fayolle, C.; Loyen, J.; Robe, M.Ch.; Picolo, J.L.; Gallerand, M.O.

    2009-01-01

    While providing many maps, graphs and tables, this report presents and comments the very large amount of data acquired in 2008 within the frame of the control by the IRSN of radioactivity levels on the French national territory by means of its different surveillance networks. After a presentation of these networks (objectives, organization, sample collection, analysis, and preparation methodologies) and of the different radionuclides present in the French environment, results are presented by installation type (electricity production nuclear centres, nuclear fuel reprocessing centres, nuclear medicine centres, nuclear waste storage centres, research centres, nuclear naval bases, etc.) and environment component (air, water ways, rain waters, continental or coastal environment, biological media, etc.)

  10. Effects of Forgetting Phenomenon on Surveillance Test Interval

    International Nuclear Information System (INIS)

    Lee, Ho-Joong; Jang, Seung-Cheol

    2007-01-01

    Technical Specifications (TS) requirements for nuclear power plants (NPPs) define Surveillance Requirements (SRs) to assure safety during operation. SRs include surveillance test intervals (STIs) and the optimization of the STIs is one of the main issues in risk-informed applications. Surveillance tests are required in NPPs to detect failures in standby equipment to assure their availability in an accident. However, operating experience of the plants suggests that, in addition to the beneficial effects of detecting latent faults, the tests also may have adverse effects on plant operation or equipment; e.g., plant transient caused by the test and wear-out of safety system equipment due to repeated testing. Recent studies have quantitatively evaluated both the beneficial and adverse effects of testing to decide on an acceptable test interval. The purpose of this research is to investigate the effects of forgetting phenomenon on STI. It is a fundamental human characteristic that a person engaged in a repetitive task will improve his performance over time. The learning phenomenon is observed by the decrease in operation time per unit as operators gain experience by performing additional tasks. However, once there is a break of sufficient length, forgetting starts to take place. In surveillance tests, the most common factor to determine the amount of forgetting is the length of STI, where the longer the STI, the greater the amount of forgetting

  11. Full-length mRNA sequencing uncovers a widespread coupling between transcription initiation and mRNA processing.

    Science.gov (United States)

    Anvar, Seyed Yahya; Allard, Guy; Tseng, Elizabeth; Sheynkman, Gloria M; de Klerk, Eleonora; Vermaat, Martijn; Yin, Raymund H; Johansson, Hans E; Ariyurek, Yavuz; den Dunnen, Johan T; Turner, Stephen W; 't Hoen, Peter A C

    2018-03-29

    The multifaceted control of gene expression requires tight coordination of regulatory mechanisms at transcriptional and post-transcriptional level. Here, we studied the interdependence of transcription initiation, splicing and polyadenylation events on single mRNA molecules by full-length mRNA sequencing. In MCF-7 breast cancer cells, we find 2700 genes with interdependent alternative transcription initiation, splicing and polyadenylation events, both in proximal and distant parts of mRNA molecules, including examples of coupling between transcription start sites and polyadenylation sites. The analysis of three human primary tissues (brain, heart and liver) reveals similar patterns of interdependency between transcription initiation and mRNA processing events. We predict thousands of novel open reading frames from full-length mRNA sequences and obtained evidence for their translation by shotgun proteomics. The mapping database rescues 358 previously unassigned peptides and improves the assignment of others. By recognizing sample-specific amino-acid changes and novel splicing patterns, full-length mRNA sequencing improves proteogenomics analysis of MCF-7 cells. Our findings demonstrate that our understanding of transcriptome complexity is far from complete and provides a basis to reveal largely unresolved mechanisms that coordinate transcription initiation and mRNA processing.

  12. Food and water radioactivity surveillance system in Romania

    International Nuclear Information System (INIS)

    Cucu, A.; Gheorghe, R.; May, C.; Barbu, R.

    2008-01-01

    Full text: Justification: Food and water radioactivity content are closely related both to natural radioactivity and also generated by contamination due to anthropic nuclear activities. Consequently, in accordance with the European Union acquis and World Health Organization recommendation, surveillance systems were operationalized in many European countries. According to the national Romanian derived legislation the public health authorities are responsible for organizing and coordination of the national surveillance system for water and food radioactivity and their health related effects. Objectives: Description of the levels and type of radioactivity of drinking water and main foodstuffs and their contribution to the Romanian population exposure in order to elaborate appropriate public health interventions. Method: The gross parameters, alpha and beta, have been used for screening surveillance of drinking water sources indeed for potable purposes in order to identify those that could exceed the total indicative dose of 0.1 mSv/year. The food surveillance was focused on the main foodstuffs including milk, meat, fish, eggs, bread, potatoes, root vegetables (mainly carrots), leafy vegetables (mainly cabbage), fruits, and canteen menu, controlled for presence and level of radioactivity for 137 Cs, 90 Sr, 226 Ra, 210 Po and 40 K. Nuclear facility related monitoring for areas as nuclear power plant Cernavoda (type HWR-CANDU) and for regions with activities of extraction and fabrication of uranium fuel includes monitoring of radioactivity for: environmental deposit levels, surface waters, spontaneous vegetation, drinking water and foodstuffs. Results: 1) The water radioactivity surveillance results, mapped by administrative borders of the national territory, reveal that parameters of drinking water complies both with Drinking Water Directive 98/83 EC and WHO recommandation/2004; 2) For food stuff radioactivity: a) Mean registered values fully comply with reference for

  13. Reprogramming the Dynamin 2 mRNA by Spliceosome-mediated RNA Trans-splicing

    Directory of Open Access Journals (Sweden)

    Delphine Trochet

    2016-01-01

    Full Text Available Dynamin 2 (DNM2 is a large GTPase, ubiquitously expressed, involved in membrane trafficking and regulation of actin and microtubule cytoskeletons. DNM2 mutations cause autosomal dominant centronuclear myopathy which is a rare congenital myopathy characterized by skeletal muscle weakness and histopathological features including nuclear centralization in absence of regeneration. No curative treatment is currently available for the DNM2-related autosomal dominant centronuclear myopathy. In order to develop therapeutic strategy, we evaluated here the potential of Spliceosome-Mediated RNA Trans-splicing technology to reprogram the Dnm2-mRNA in vitro and in vivo in mice. We show that classical 3′-trans-splicing strategy cannot be considered as accurate therapeutic strategy regarding toxicity of the pre-trans-splicing molecules leading to low rate of trans-splicing in vivo. Thus, we tested alternative strategies devoted to prevent this toxicity and enhance frequency of trans-splicing events. We succeeded to overcome the toxicity through a 5′-trans-splicing strategy which also allows detection of trans-splicing events at mRNA and protein levels in vitro and in vivo. These results suggest that the Spliceosome-Mediated RNA Trans-splicing strategy may be used to reprogram mutated Dnm2-mRNA but highlight the potential toxicity linked to the molecular tools which have to be carefully investigated during preclinical development.

  14. Localization of glucocorticoid receptor mRNA in the male rat brain by in situ hybridization

    International Nuclear Information System (INIS)

    Aronsson, M.; Fuxe, K.; Dong, Y.; Agnati, L.F.; Okret, S.; Gustafsson, J.A.

    1988-01-01

    The localization and distribution of mRNA encoding the glucocorticoid receptor (GR) was investigated in tissue sections of the adult male rat brain by in situ hybridization and RNA blot analysis. GR mRNA levels were measured by quantitative autoradiography with 35S- and 32P-labeled RNA probes, respectively. Strong labeling was observed within the pyramidal nerve cells of the CA1 and CA2 areas of the hippocampal formation, in the granular cells of the dentate gyrus, in the parvocellular nerve cells of the paraventricular hypothalamic nucleus, and in the cells of the arcuate nucleus, especially the parvocellular part. Moderate labeling of a large number of nerve cells was observed within layers II, III, and VI of the neocortex and in many thalamic nuclei, especially the anterior and ventral nuclear groups as well as several midline nuclei. Within the cerebellar cortex, strong labeling was observed all over the granular layer. In the lower brainstem, strong labeling was found within the entire locus coeruleus and within the mesencephalic raphe nuclei rich in noradrenaline and 5-hydroxytryptamine cell bodies, respectively. A close correlation was found between the distribution of GR mRNA and the distribution of previously described GR immunoreactivity. These studies open the possibility of obtaining additional information on in vivo regulation of GR synthesis and how the brain may alter its sensitivity to circulating glucocorticoids

  15. Role of the mRNA export factor Sus1 in oxidative stress tolerance in Candida albicans.

    Science.gov (United States)

    Xiao, Chenpeng; Yu, Qilin; Zhang, Bing; Li, Jianrong; Zhang, Dan; Li, Mingchun

    2018-02-05

    In eukaryotes, the nuclear export of mRNAs is essential for gene expression. However, little is known about the role of mRNA nuclear export in the important fungal pathogen, Candida albicans. In this study, we identified C. albicans Sus1, a nucleus-localized protein that is required for mRNA export. Interestingly, the sus1Δ/Δ displayed hyper-sensitivity to extracellular oxidative stress, enhanced ROS accumulation and severe oxidative stress-related cell death. More strikingly, although the mutant exhibited normal activation of the expression of oxidative stress response (OSR) genes, it had attenuated activity of ROS scavenging system, which may be attributed to the defect in OSR mRNA export in this mutant. In addition, the virulence of the sus1Δ/Δ was seriously attenuated. Taken together, our findings provide evidence that the mRNA export factor Sus1 plays an important role in oxidative stress tolerance and pathogenesis. Copyright © 2018 Elsevier Inc. All rights reserved.

  16. Regional Disease Surveillance Meeting - Final Paper

    Energy Technology Data Exchange (ETDEWEB)

    Lesperance, Ann M.; Mahy, Heidi A.

    2006-08-08

    On June 1, 2006, public health officials working in surveillance, epidemiological modeling, and information technology communities from the Seattle/Tacoma area and State of Washington met with members of the Pacific Northwest National Laboratory (PNNL) to discuss the current state of disease surveillance and gaps and needs to improve the current systems. The meeting also included a discussion of PNNL initiatives that might be appropriate to enhance disease surveillance and the current tools being used for disease surveillance. Participants broke out into two groups to identify critical gaps and needs for improving a surveillance system, and discuss the requirements for developing improved surveillance. Each group developed a list of key priorities summarizing the requirements for improved surveillance. The objective of this meeting was to work towards the development of an improved disease surveillance system.

  17. Inappropriate colonoscopic surveillance of hyperplastic polyps.

    LENUS (Irish Health Repository)

    Keane, R A

    2011-11-15

    Colonoscopic surveillance of hyperplastic polyps alone is controversial and may be inappropriate. The colonoscopy surveillance register at a university teaching hospital was audited to determine the extent of such hyperplastic polyp surveillance. The surveillance endoscopy records were reviewed, those patients with hyperplastic polyps were identified, their clinical records were examined and contact was made with each patient. Of the 483 patients undergoing surveillance for colonic polyps 113 (23%) had hyperplastic polyps alone on last colonoscopy. 104 patients remained after exclusion of those under appropriate surveillance. 87 of the 104 patients (84%) were successfully contacted. 37 patients (8%) were under appropriate colonoscopic surveillance for a significant family history of colorectal carcinoma. 50 (10%) patients with hyperplastic polyps alone and no other clinical indication for colonoscopic surveillance were booked for follow up colonoscopy. This represents not only a budgetary but more importantly a clinical opportunity cost the removal of which could liberate valuable colonoscopy time for more appropriate indications.

  18. National Cardiac Device Surveillance Program Database

    Data.gov (United States)

    Department of Veterans Affairs — The National Cardiac Device Surveillance Program Database supports the Eastern Pacemaker Surveillance Center (EPSC) staff in its function of monitoring some 11,000...

  19. Process instrument monitoring for SNM solution surveillance

    International Nuclear Information System (INIS)

    Armatys, C.M.; Johnson, C.E.; Wagner, E.P.

    1983-02-01

    A process monitoring computer system at the Idaho Chemical Processing Plant (ICPP) is being used to evaluate nuclear fuel reprocessing plant data for Safeguards surveillance capabilities. The computer system was installed to collect data from the existing plant instruments and to evaluate what safeguards assurances can be provided to complement conventional accountability and physical protection measures. Movements of solutions containing special nuclear material (SNM) can be observed, activities associated with accountancy measurements (mixing, sampling, and bulk measurement) can be confirmed, and long-term storage of SNM solutions can be monitored to ensure containment. Special precautions must be taken, both in system design and operation to ensure adequate coverage of essential measured parameters and interpretation of process data, which can be comprised by instrument malfunctions or failures, unreliable data collection, or process activities that deviate from readily identified procedures. Experience at ICPP and prior evaluations at the Tokai reprocessing plant show that the use of process data can provide assurances that accountability measurement procedures are followed and SNM solutions are properly contained and can help confirm that SNM controls are in effect within a facility

  20. Surveillance by diagnostic microbiology laboratories

    African Journals Online (AJOL)

    account for almost threequarters of all Acinetobacter baumannii bloodstream infections, supporting the decision to include colistin or tobramycin as empirical treatment options for ICU patients with suspected Gramnegative sepsis. The dissemination and utilisation of surveillance data is crucial if they are to impact on patient ...

  1. Symbolic power, robotting, and surveilling

    DEFF Research Database (Denmark)

    Skovsmose, Ole

    2012-01-01

    describes as it prioritises is discussed with reference to robotting and surveillance. In general, the symbolic power of mathematics and formal languages is summarised through the observations: that mathematics treats parts and properties as autonomous, that it dismembers what it addresses and destroys...

  2. Nuclear safeguards control in nuclear power stations

    International Nuclear Information System (INIS)

    Boedege, R.; Braatz, U.; Heger, H.

    1976-01-01

    The execution of the Non-Proliferation Treaty (NPT) has initiated a third phase in the efforts taken to ensure peace by limiting the number of atomic powers. In this phase it is important, above all, to turn into workable systems the conditions imposed upon technology by the different provisions of the Verification Agreement of the NPT. This is achieved mainly by elaborating annexes to the Agreement specifically geared to certain model plants, typical representatives selected for LWR power stations being the plants at Garigliano, Italy (BWR), and Stade, Federal Republic of Germany (PWR). The surveillance measures taken to prevent any diversion of special nuclear material for purposes of nuclear weapons manufacture must be effective in achieving their specific objective and must not impede the circumspect management of operations of the plants concerned. A VDEW working party has studied the technical details of the planned surveillance measures in nuclear power stations in the Federal Republic of Germany and now presents a concept of material balancing by units which meets the conditions imposed by the inspection authority and could also be accepted by the operators of nuclear power stations. The concept provides for uninterrupted control of the material balance areas of the nuclear power stations concerned, allows continuous control of the whole nuclear fuel cycle, is based exclusively on existing methods and facilities, and can be implemented at low cost. (orig.) [de

  3. Dis3- and exosome subunit-responsive 3′ mRNA instability elements

    International Nuclear Information System (INIS)

    Kiss, Daniel L.; Hou, Dezhi; Gross, Robert H.; Andrulis, Erik D.

    2012-01-01

    Highlights: ► Successful use of a novel RNA-specific bioinformatic tool, RNA SCOPE. ► Identified novel 3′ UTR cis-acting element that destabilizes a reporter mRNA. ► Show exosome subunits are required for cis-acting element-mediated mRNA instability. ► Define precise sequence requirements of novel cis-acting element. ► Show that microarray-defined exosome subunit-regulated mRNAs have novel element. -- Abstract: Eukaryotic RNA turnover is regulated in part by the exosome, a nuclear and cytoplasmic complex of ribonucleases (RNases) and RNA-binding proteins. The major RNase of the complex is thought to be Dis3, a multi-functional 3′–5′ exoribonuclease and endoribonuclease. Although it is known that Dis3 and core exosome subunits are recruited to transcriptionally active genes and to messenger RNA (mRNA) substrates, this recruitment is thought to occur indirectly. We sought to discover cis-acting elements that recruit Dis3 or other exosome subunits. Using a bioinformatic tool called RNA SCOPE to screen the 3′ untranslated regions of up-regulated transcripts from our published Dis3 depletion-derived transcriptomic data set, we identified several motifs as candidate instability elements. Secondary screening using a luciferase reporter system revealed that one cassette—harboring four elements—destabilized the reporter transcript. RNAi-based depletion of Dis3, Rrp6, Rrp4, Rrp40, or Rrp46 diminished the efficacy of cassette-mediated destabilization. Truncation analysis of the cassette showed that two exosome subunit-sensitive elements (ESSEs) destabilized the reporter. Point-directed mutagenesis of ESSE abrogated the destabilization effect. An examination of the transcriptomic data from exosome subunit depletion-based microarrays revealed that mRNAs with ESSEs are found in every up-regulated mRNA data set but are underrepresented or missing from the down-regulated data sets. Taken together, our findings imply a potentially novel mechanism of mRNA

  4. Human apolipoprotein B (apoB) mRNA: Identification of two distinct apoB mRNAs, an mRNA with the apoB-100 sequence and an apoB mRNA containing a premature in-frame translational stop codon, in both liver and intestine

    International Nuclear Information System (INIS)

    Higuchi, K.; Hospattankar, A.V.; Law, S.W.; Meglin, N.; Cortright, J.; Brewer, H.B. Jr.

    1988-01-01

    Human apolipoprotein B (apoB) is present in plasma as two separate isoproteins, designated apoB-100 (512 kDa) and apoB-48 (250 kDa). ApoB is encoded by a single gene on chromosome 2, and a single nuclear mRNA is edited and processed into two separate apoB mRNAs. A 14.1-kilobase apoB mRNA codes for apoB-100, and the second mRNA, which codes for apoB-48, contains a premature stop codon generated by a single base substitution of cytosine to uracil at nucleotide 6,538, which converts the translated CAA codon coding for the amino acid glutamine at residue 2,153 in apoB-100 to a premature in-frame stop codon (UAA). Two 30-base synthetic oligonucleotides, designated apoB-Stop and apoB-Gln, were synthesized containing the complementary sequence to the stop codon (UAA) and glutamine codon (CAA), respectively. The combined results from these studies establish that both human intestine and liver contain the two distinct apoB mRNAs, an mRNA that codes for apoB-100 and an apoB mRNA that contains the premature stop codon, which codes for apoB-48. The premature in-frame stop codon is not tissue specific and is present in both human liver and intestine

  5. Expression of calmodulin mRNA in rat olfactory neuroepithelium.

    Science.gov (United States)

    Biffo, S; Goren, T; Khew-Goodall, Y S; Miara, J; Margolis, F L

    1991-04-01

    A calmodulin (CaM) cDNA was isolated by differential hybridization screening of a lambda gt10 library prepared from rat olfactory mucosa. This cDNA fragment, containing most of the open reading frame of the rat CaMI gene, was subcloned and used to characterize steady-state expression of CaM mRNA in rat olfactory neuroepithelium and bulb. Within the bulb mitral cells are the primary neuronal population expressing CaM mRNA. The major CaM mRNA expressed in the olfactory mucosa is 1.7 kb with smaller contributions from mRNAs of 4.0 and 1.4 kb. CaM mRNA was primarily associated with the olfactory neurons and, despite the cellular complexity of the tissue and the known involvement of CaM in diverse cellular processes, was only minimally evident in sustentacular cells, gland cells or respiratory epithelium. Following bulbectomy CaM mRNA declines in the olfactory neuroepithelium as does olfactory marker protein (OMP) mRNA. In contrast to the latter, CaM mRNA makes a partial recovery by one month after surgery. These results, coupled with those from in situ hybridization, indicate that CaM mRNA is expressed in both mature and immature olfactory neurons. The program regulating CaM gene expression in olfactory neurons is distinct from those controlling expression of B50/GAP43 in immature, or OMP in mature, neurons respectively.

  6. Approaches to canine health surveillance.

    Science.gov (United States)

    O'Neill, Dan G; Church, David B; McGreevy, Paul D; Thomson, Peter C; Brodbelt, Dave C

    2014-01-01

    Effective canine health surveillance systems can be used to monitor disease in the general population, prioritise disorders for strategic control and focus clinical research, and to evaluate the success of these measures. The key attributes for optimal data collection systems that support canine disease surveillance are representativeness of the general population, validity of disorder data and sustainability. Limitations in these areas present as selection bias, misclassification bias and discontinuation of the system respectively. Canine health data sources are reviewed to identify their strengths and weaknesses for supporting effective canine health surveillance. Insurance data benefit from large and well-defined denominator populations but are limited by selection bias relating to the clinical events claimed and animals covered. Veterinary referral clinical data offer good reliability for diagnoses but are limited by referral bias for the disorders and animals included. Primary-care practice data have the advantage of excellent representation of the general dog population and recording at the point of care by veterinary professionals but may encounter misclassification problems and technical difficulties related to management and analysis of large datasets. Questionnaire surveys offer speed and low cost but may suffer from low response rates, poor data validation, recall bias and ill-defined denominator population information. Canine health scheme data benefit from well-characterised disorder and animal data but reflect selection bias during the voluntary submissions process. Formal UK passive surveillance systems are limited by chronic under-reporting and selection bias. It is concluded that active collection systems using secondary health data provide the optimal resource for canine health surveillance.

  7. Environmental surveillance master sampling schedule

    International Nuclear Information System (INIS)

    Bisping, L.E.

    1995-02-01

    Environmental surveillance of the Hanford Site and surrounding areas is conducted by the Pacific Northwest Laboratory (PNL) for the U.S. Department of Energy (DOE). This document contains the planned 1994 schedules for routine collection of samples for the Surface Environmental Surveillance Project (SESP), Drinking Water Project, and Ground-Water Surveillance Project. Samples are routinely collected for the SESP and analyzed to determine the quality of air, surface water, soil, sediment, wildlife, vegetation, foodstuffs, and farm products at Hanford Site and surrounding communities. The responsibility for monitoring onsite drinking water falls outside the scope of the SESP. PNL conducts the drinking water monitoring project concurrent with the SESP to promote efficiency and consistency, utilize expertise developed over the years, and reduce costs associated with management, procedure development, data management, quality control, and reporting. The ground-water sampling schedule identifies ground-water sampling .events used by PNL for environmental surveillance of the Hanford Site. Sampling is indicated as annual, semi-annual, quarterly, or monthly in the sampling schedule. Some samples are collected and analyzed as part of ground-water monitoring and characterization programs at Hanford (e.g. Resources Conservation and Recovery Act (RCRA), Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA), or Operational). The number of samples planned by other programs are identified in the sampling schedule by a number in the analysis column and a project designation in the Cosample column. Well sampling events may be merged to avoid redundancy in cases where sampling is planned by both-environmental surveillance and another program

  8. Environmental surveillance master sampling schedule

    Energy Technology Data Exchange (ETDEWEB)

    Bisping, L.E.

    1995-02-01

    Environmental surveillance of the Hanford Site and surrounding areas is conducted by the Pacific Northwest Laboratory (PNL) for the U.S. Department of Energy (DOE). This document contains the planned 1994 schedules for routine collection of samples for the Surface Environmental Surveillance Project (SESP), Drinking Water Project, and Ground-Water Surveillance Project. Samples are routinely collected for the SESP and analyzed to determine the quality of air, surface water, soil, sediment, wildlife, vegetation, foodstuffs, and farm products at Hanford Site and surrounding communities. The responsibility for monitoring onsite drinking water falls outside the scope of the SESP. PNL conducts the drinking water monitoring project concurrent with the SESP to promote efficiency and consistency, utilize expertise developed over the years, and reduce costs associated with management, procedure development, data management, quality control, and reporting. The ground-water sampling schedule identifies ground-water sampling .events used by PNL for environmental surveillance of the Hanford Site. Sampling is indicated as annual, semi-annual, quarterly, or monthly in the sampling schedule. Some samples are collected and analyzed as part of ground-water monitoring and characterization programs at Hanford (e.g. Resources Conservation and Recovery Act (RCRA), Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA), or Operational). The number of samples planned by other programs are identified in the sampling schedule by a number in the analysis column and a project designation in the Cosample column. Well sampling events may be merged to avoid redundancy in cases where sampling is planned by both-environmental surveillance and another program.

  9. Proteomic analysis of ACTN4-interacting proteins reveals it's a putative involvement in mRNA metabolism

    International Nuclear Information System (INIS)

    Khotin, Mikhail; Turoverova, Lidia; Aksenova, Vasilisa; Barlev, Nikolai; Borutinskaite, Veronika Viktorija; Vener, Alexander; Bajenova, Olga; Magnusson, Karl-Eric; Pinaev, George P.; Tentler, Dmitri

    2010-01-01

    Alpha-actinin 4 (ACTN4) is an actin-binding protein. In the cytoplasm, ACTN4 participates in structural organisation of the cytoskeleton via cross-linking of actin filaments. Nuclear localisation of ACTN4 has also been reported, but no clear role in the nucleus has been established. In this report, we describe the identification of proteins associated with ACTN4 in the nucleus. A combination of two-dimensional gel electrophoresis (2D-GE) and MALDI-TOF mass-spectrometry revealed a large number of ACTN4-bound proteins that are involved in various aspects of mRNA processing and transport. The association of ACTN4 with different ribonucleoproteins suggests that a major function of nuclear ACTN4 may be regulation of mRNA metabolism and signaling.

  10. mRNA export in the apicomplexan parasite Toxoplasma gondii: emerging divergent components of a crucial pathway.

    Science.gov (United States)

    Ávila, Andréa Rodrigues; Cabezas-Cruz, Alexjandro; Gissot, Mathieu

    2018-01-25

    Control of gene expression is crucial for parasite survival and is the result of a series of processes that are regulated to permit fine-tuning of gene expression in response to biological changes during the life-cycle of apicomplexan parasites. Control of mRNA nuclear export is a key process in eukaryotic cells but is poorly understood in apicomplexan parasites. Here, we review recent knowledge regarding this process with an emphasis on T. gondii. We describe the presence of divergent orthologs and discuss structural and functional differences in export factors between apicomplexans and other eukaryotic lineages. Undoubtedly, the use of the CRISPR/Cas9 system in high throughput screenings associated with the discovery of mRNA nuclear export complexes by proteomic analysis will contribute to identify these divergent factors. Ligand-based or structure-based strategies may be applied to investigate the potential use of these proteins as targets for new antiprotozoal agents.

  11. SCORPIO: a framework for core surveillance systems

    International Nuclear Information System (INIS)

    Berg, Oe.; Tsuiki, Makoto

    1999-01-01

    The first version of the core surveillance system SCORPIO was installed at Unit 2, Ringhals, in 1984. It was implemented on Norsk Data mini-computers with a fully graphical user-interface. The main purpose was to provide a practical tool for reactor operators and reactor physicists for on-line monitoring and predictive analysis of core behaviour. A second version of SCORPIO was developed in 1993-1995 and implemented on Unix workstations. In addition to upgrading the system at Ringhals, the system was installed by Duke Power, USA, on 7 reactors. SCORPIO was also installed on the Sizewell B reactor. Recently a new framework has been developed which further enhances the flexibility and capabilities for implementing core surveillance systems in different types of nuclear power plants. Modules can be added and replaced in an easy manner. It allows fast and reliable communication of data between modules based on the Software Bus tool developed by IFE. Further, the Picasso-3 user interface management system supports efficient implementation of different user interfaces. Both Unix and Windows NT platforms are supported. The new framework has been applied in development and installation of a SCORPIO-VVER version for the Dukovany NPP, Czech Republic. Here it was of particular importance to provide a flexible system for integration of modules originating from different companies. Development of a BWR version is now in progress. This means that SCORPIO will be available for all the major reactor types, and synergy is obtained by application of a common framework both with respect to system implementation and maintenance. By using the SCORPIO framework, the development time is reduced and the maintenance work is carried out more efficiently, compared to developing systems with lower-level tools. For instance, the MMI can be developed and tested independently of the physics modules

  12. Environmental health surveillance system; Kankyo hoken surveillance system

    Energy Technology Data Exchange (ETDEWEB)

    Ono, M. [National Inst. for Environmental Studies, Tsukuba (Japan)

    1998-02-01

    The Central Environmental Pollution Prevention Council pointed out the necessity to establish an environmental health surveillance system (hereinafter referred to as System) in its report `on the first type district specified by the Environmental Pollution Caused Health Damages Compensation Act,` issued in 1986. A study team, established in Environment Agency, has been discussing to establish System since 1986. This paper outlines System, and some of the pilot surveillance results. It is not aimed at elucidation of the cause-effect relationships between health and air pollution but at discovery of problems, in which the above relationships in a district population are monitored periodically and continuously from long-term and prospective viewpoints, in order to help take necessary measures in the early stage. System is now collecting the data of the chronic obstructive lung diseases on a nation-wide scale through health examinations of 3-year-old and preschool children and daily air pollution monitoring. 6 refs., 3 figs., 1 tab.

  13. Decree of the Czechoslovak Atomic Energy Commission concerning the security protection of nuclear installations and nuclear materials

    International Nuclear Information System (INIS)

    1989-01-01

    In compliance with the Czechoslovak State Surveillance over Nuclear Safety of Nuclear Installations Act No. 28/1984, the Decree specifies requirements for assuring security protection of nuclear installations (and their parts) and of nuclear materials with the aim to prevent their abuse for jeopardizing the environment and the health and lives of people. (P.A.)

  14. Public involvement in environmental surveillance at Hanford

    International Nuclear Information System (INIS)

    Hanf, R.W. Jr.; Patton, G.W.; Woodruff, R.K.; Poston, T.M.

    1994-08-01

    Environmental surveillance at the Hanford Site began during the mid-1940s following the construction and start-up of the nation's first plutonium production reactor. Over the past approximately 45 years, surveillance operations on and off the Site have continued, with virtually all sampling being conducted by Hanford Site workers. Recently, the US Department of Energy Richland Operations Office directed that public involvement in Hanford environmental surveillance operations be initiated. Accordingly, three special radiological air monitoring stations were constructed offsite, near hanford's perimeter. Each station is managed and operated by two local school teaches. These three stations are the beginning of a community-operated environmental surveillance program that will ultimately involve the public in most surveillance operations around the Site. The program was designed to stimulate interest in Hanford environmental surveillance operations, and to help the public better understand surveillance results. The program has also been used to enhance educational opportunities at local schools

  15. Issues ignored in laboratory quality surveillance

    International Nuclear Information System (INIS)

    Zeng Jing; Li Xingyuan; Zhang Tingsheng

    2008-01-01

    According to the work requirement of the related laboratory quality surveillance in ISO17025, this paper analyzed and discussed the issued ignored in the laboratory quality surveillance. In order to solve the present problem, it is required to understand the work responsibility in the quality surveillance correctly, to establish the effective working routine in the quality surveillance, and to conduct, the quality surveillance work. The object in the quality surveillance shall be 'the operator' who engaged in the examination/calibration directly in the laboratory, especially the personnel in training (who is engaged in the examination/calibration). The quality supervisors shall be fully authorized, so that they can correctly understand the work responsibility in quality surveillance, and are with the rights for 'full supervision'. The laboratory also shall arrange necessary training to the quality supervisor, so that they can obtain sufficient guide in time and are with required qualification or occupation prerequisites. (authors)

  16. Risk based surveillance for vector borne diseases

    DEFF Research Database (Denmark)

    Bødker, Rene

    of samples and hence early detection of outbreaks. Models for vector borne diseases in Denmark have demonstrated dramatic variation in outbreak risk during the season and between years. The Danish VetMap project aims to make these risk based surveillance estimates available on the veterinarians smart phones...... in Northern Europe. This model approach may be used as a basis for risk based surveillance. In risk based surveillance limited resources for surveillance are targeted at geographical areas most at risk and only when the risk is high. This makes risk based surveillance a cost effective alternative...... sample to a diagnostic laboratory. Risk based surveillance models may reduce this delay. An important feature of risk based surveillance models is their ability to continuously communicate the level of risk to veterinarians and hence increase awareness when risk is high. This is essential for submission...

  17. Reactor Vessel Surveillance Program for Advanced Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Jeong, Kyeong-Hoon; Kim, Tae-Wan; Lee, Gyu-Mahn; Kim, Jong-Wook; Park, Keun-Bae; Kim, Keung-Koo

    2008-10-15

    This report provides the design requirements of an integral type reactor vessel surveillance program for an integral type reactor in accordance with the requirements of Korean MEST (Ministry of Education, Science and Technology Development) Notice 2008-18. This report covers the requirements for the design of surveillance capsule assemblies including their test specimens, test block materials, handling tools, and monitors of the surveillance capsule neutron fluence and temperature. In addition, this report provides design requirements for the program for irradiation surveillance of reactor vessel materials, a layout of specimens and monitors in the surveillance capsule, procedures of installation and retrieval of the surveillance capsule assemblies, and the layout of the surveillance capsule assemblies in the reactor.

  18. Health effects and medical surveillance

    International Nuclear Information System (INIS)

    1998-01-01

    Source of ionizing radiations have innumerable applications in the work place. Usually, even where the work is performed safely, the employees involved inevitably receive small, regular exposures to radiation that are not manifestly harmful. This Module explains how ionizing radiations can interact with and affect human tissues, the various factors that influence the outcome and the detrimental effects that may result. The medical surveillance that is appropriate for those working with radiation sources, depending on the degree of hazard of the work, is described. The Manual will be of most benefit it if forms part of more comprehensive training or is supplemented by the advice of a medically qualified expert. Where medical surveillance is appropriate for radiation employees, the services of a qualified doctor, occupational physician or other trained medical staff will be required

  19. Bat Rabies Surveillance in Europe

    DEFF Research Database (Denmark)

    Schatz, J.; Fooks, A. R.; McElhinney, L.

    2013-01-01

    Rabies is the oldest known zoonotic disease and was also the first recognized bat associated infection in humans. To date, four different lyssavirus species are the causative agents of rabies in European bats: the European Bat Lyssaviruses type 1 and 2 (EBLV-1, EBLV-2), the recently discovered...... putative new lyssavirus species Bokeloh Bat Lyssavirus (BBLV) and the West Caucasian Bat Virus (WCBV). Unlike in the new world, bat rabies cases in Europe are comparatively less frequent, possibly as a result of varying intensity of surveillance. Thus, the objective was to provide an assessment of the bat...... rabies surveillance data in Europe, taking both reported data to the WHO Rabies Bulletin Europe and published results into account. In Europe, 959 bat rabies cases were reported to the RBE in the time period 1977–2010 with the vast majority characterized as EBLV-1, frequently isolated in the Netherlands...

  20. Environmental surveillance master sampling schedule

    International Nuclear Information System (INIS)

    Bisping, L.E.

    1997-01-01

    Environmental surveillance of the Hanford Site and surrounding areas is conducted by the Pacific Northwest National Laboratory (PNNL)(a) for the US Department of Energy (DOE). This document contains the planned 1997 schedules for routine collection of samples for the Surface Environmental Surveillance Project (SESP) and Drinking Water Monitoring Project. In addition, Section 3.0, Biota, also reflects a rotating collection schedule identifying the year a specific sample is scheduled for collection. The purpose of these monitoring projects is to evaluate levels of radioactive and nonradioactive pollutants in the Hanford environs, as required in DOE Order 5400.1, General Environmental Protection Program, and DOE Order 5400.5, Radiation Protection of the Public and the Environment. The sampling methods will be the same as those described in the Environmental Monitoring Plan, US Department of Energy, Richland Operations Office, DOE/RL91-50, Rev. 1, US Department of Energy, Richland, Washington

  1. Mining Surveillance and Maintenance Dollars

    International Nuclear Information System (INIS)

    MARTINEZ, R.

    2000-01-01

    Accelerating site cleanup to reduce facility risks to the workers, the public and the environment during a time of declining federal budgets represents a significant technical and economic challenge to U.S. Department of Energy (DOE) Operations Offices and their respective contractors. A significant portion of a facility's recurring annual expenses are associated with routine, long-term surveillance and maintenance (S and M) activities. However, ongoing S and M activities do nothing to reduce risks and basically spend money that could be reallocated towards facility deactivation. This paper discusses the background around DOE efforts to reduce surveillance and maintenance costs, one approach used to perform cost reviews, lessons learned from field implementation and what assistance is available to assist DOE sites in performing these evaluations

  2. Environmental surveillance master sampling schedule

    Energy Technology Data Exchange (ETDEWEB)

    Bisping, L E

    1992-01-01

    Environmental surveillance of the Hanford Site and surrounding areas is conducted by the Pacific Northwest Laboratory (PNL) for the US Department of Energy (DOE). This document contains the planned schedule for routine sample collection for the Surface Environmental Surveillance Project (SESP) and Ground-Water Monitoring Project. Samples for radiological analyses include Air-Particulate Filter, gases and vapor; Water/Columbia River, Onsite Pond, Spring, Irrigation, and Drinking; Foodstuffs/Animal Products including Whole Milk, Poultry and Eggs, and Beef; Foodstuffs/Produce including Leafy Vegetables, Vegetables, and Fruit; Foodstuffs/Farm Products including Wine, Wheat and Alfalfa; Wildlife; Soil; Vegetation; and Sediment. Direct Radiation Measurements include Terrestrial Locations, Columbia River Shoreline Locations, and Onsite Roadway, Railway and Aerial, Radiation Surveys.

  3. Environmental surveillance master sampling schedule

    International Nuclear Information System (INIS)

    Bisping, L.E.

    1994-02-01

    This document contains the planned 1994 schedules for routine collection of samples for the Surface Environmental Surveillance Project (SESP), Drinking Water Project, and Ground-Water Surveillance Project. Samples are routinely collected for the SESP and analyzed to determine the quality of air, surface water, soil, sediment, wildlife, vegetation, foodstuffs, and farm products at Hanford Site and surrounding communities. The responsibility for monitoring the onsite drinking water falls outside the scope of the SESP. The Hanford Environmental Health Foundation is responsible for monitoring the nonradiological parameters as defined in the National Drinking Water Standards while PNL conducts the radiological monitoring of the onsite drinking water. PNL conducts the drinking water monitoring project concurrent with the SESP to promote efficiency and consistency, utilize the expertise developed over the years, and reduce costs associated with management, procedure development, data management, quality control and reporting. The ground-water sampling schedule identifies ground-water sampling events used by PNL for environmental surveillance of the Hanford Site

  4. Environmental surveillance master sampling schedule

    Energy Technology Data Exchange (ETDEWEB)

    Bisping, L.E.

    1994-02-01

    This document contains the planned 1994 schedules for routine collection of samples for the Surface Environmental Surveillance Project (SESP), Drinking Water Project, and Ground-Water Surveillance Project. Samples are routinely collected for the SESP and analyzed to determine the quality of air, surface water, soil, sediment, wildlife, vegetation, foodstuffs, and farm products at Hanford Site and surrounding communities. The responsibility for monitoring the onsite drinking water falls outside the scope of the SESP. The Hanford Environmental Health Foundation is responsible for monitoring the nonradiological parameters as defined in the National Drinking Water Standards while PNL conducts the radiological monitoring of the onsite drinking water. PNL conducts the drinking water monitoring project concurrent with the SESP to promote efficiency and consistency, utilize the expertise developed over the years, and reduce costs associated with management, procedure development, data management, quality control and reporting. The ground-water sampling schedule identifies ground-water sampling events used by PNL for environmental surveillance of the Hanford Site.

  5. mRNA localization mechanisms in Trypanosoma cruzi.

    Directory of Open Access Journals (Sweden)

    Lysangela R Alves

    Full Text Available Asymmetric mRNA localization is a sophisticated tool for regulating and optimizing protein synthesis and maintaining cell polarity. Molecular mechanisms involved in the regulated localization of transcripts are widespread in higher eukaryotes and fungi, but not in protozoa. Trypanosomes are ancient eukaryotes that branched off early in eukaryote evolution. We hypothesized that these organisms would have basic mechanisms of mRNA localization. FISH assays with probes against transcripts coding for proteins with restricted distributions showed a discrete localization of the mRNAs in the cytoplasm. Moreover, cruzipain mRNA was found inside reservosomes suggesting new unexpected functions for this vacuolar organelle. Individual mRNAs were also mobilized to RNA granules in response to nutritional stress. The cytoplasmic distribution of these transcripts changed with cell differentiation, suggesting that localization mechanisms might be involved in the regulation of stage-specific protein expression. Transfection assays with reporter genes showed that, as in higher eukaryotes, 3'UTRs were responsible for guiding mRNAs to their final location. Our results strongly suggest that Trypanosoma cruzi have a core, basic mechanism of mRNA localization. This kind of controlled mRNA transport is ancient, dating back to early eukaryote evolution.

  6. Attaching Hollywood to a Surveillant Assemblage: Normalizing Discourses of Video Surveillance

    Directory of Open Access Journals (Sweden)

    Randy K Lippert

    2015-10-01

    Full Text Available This article examines video surveillance images in Hollywood film. It moves beyond previous accounts of video surveillance in relation to film by theoretically situating the use of these surveillance images in a broader “surveillant assemblage”. To this end, scenes from a sample of thirty-five (35 films of several genres are examined to discern dominant discourses and how they lend themselves to normalization of video surveillance. Four discourses are discovered and elaborated by providing examples from Hollywood films. While the films provide video surveillance with a positive associative association it is not without nuance and limitations. Thus, it is found that some forms of resistance to video surveillance are shown while its deterrent effect is not. It is ultimately argued that Hollywood film is becoming attached to a video surveillant assemblage discursively through these normalizing discourses as well as structurally to the extent actual video surveillance technology to produce the images is used.

  7. Development of the new data transmission and processing equipment for radiation surveillance

    International Nuclear Information System (INIS)

    Suzuki, Shintaro; Takahashi, Kouichi; Suganami, Jun; Kawai, Toshiaki

    2004-01-01

    In the Mito Atomic Energy Office, which belongs to Ministry of Education, Culture, Sports, Science and Technology, as part of an environmental safety measures of the nuclear institutions in Ibaraki area, the regular surveillance of the environmental monitoring data measured in Japan Nuclear Cycle Development Institute (JNC) and Japan Atomic Energy Research Institute (JAERI) which are main facilities in Oarai and Tokai area is performed. For the purpose of strengthening environmental radiation surveillance in the fiscal year 2003, the data transmission and processing equipment for radiation surveillance is updated, and the new equipment is actually operated from March, 2004. In this paper, the features and functions of the new data transmission and processing equipment are introduced. (author)

  8. HIV surveillance in complex emergencies.

    Science.gov (United States)

    Salama, P; Dondero, T J

    2001-04-01

    Many studies have shown a positive association between both migration and temporary expatriation and HIV risk. This association is likely to be similar or even more pronounced for forced migrants. In general, HIV transmission in host-migrant or host-forced-migrant interactions depends on the maturity of the HIV epidemic in both the host and the migrant population, the relative seroprevalence of HIV in the host and the migrant population, the prevalence of other sexually transmitted infections (STIs) that may facilitate transmission, and the level of sexual interaction between the two communities. Complex emergencies are the major cause of mass population movement today. In complex emergencies, additional factors such as sexual interaction between forced-migrant populations and the military; sexual violence; increasing commercial sex work; psychological trauma; and disruption of preventive and curative health services may increase the risk for HIV transmission. Despite recent success in preventing HIV infection in stable populations in selected developing countries, internally displaced persons and refugees (or forced migrants) have not been systematically included in HIV surveillance systems, nor consequently in prevention activities. Standard surveillance systems that rely on functioning health services may not provide useful data in many complex emergency settings. Secondary sources can provide some information in these settings. Little attempt has been made, however, to develop innovative HIV surveillance systems in countries affected by complex emergencies. Consequently, data on the HIV epidemic in these countries are scarce and HIV prevention programs are either not implemented or interventions are not effectively targeted. Second generation surveillance methods such as cross-sectional, population-based surveys can provide rapid information on HIV, STIs, and sexual behavior. The risks for stigmatization and breaches of confidentiality must be recognized

  9. Protein Structure and the Sequential Structure of mRNA

    DEFF Research Database (Denmark)

    Brunak, Søren; Engelbrecht, Jacob

    1996-01-01

    entries in the Brookhaven Protein Data Bank produced 719 protein chains with matching mRNA sequence, amino acid sequence, and secondary structure assignment, By neural network analysis, we found strong signals in mRNA sequence regions surrounding helices and sheets, These signals do not originate from......A direct comparison of experimentally determined protein structures and their corresponding protein coding mRNA sequences has been performed, We examine whether real world data support the hypothesis that clusters of rare codons correlate with the location of structural units in the resulting...... protein, The degeneracy of the genetic code allows for a biased selection of codons which may control the translational rate of the ribosome, and may thus in vivo have a catalyzing effect on the folding of the polypeptide chain, A complete search for GenBank nucleotide sequences coding for structural...

  10. Increased IL-10 mRNA and IL-23 mRNA expression in multiple sclerosis: interferon-beta treatment increases IL-10 mRNA expression while reducing IL-23 mRNA expression

    DEFF Research Database (Denmark)

    Krakauer, M.; Sorensen, P.; Khademi, M.

    2008-01-01

    volunteers served to confirm initial findings. mRNA was analyzed by real-time reverse transcriptase polymerase chain reaction (PCR). RESULTS: We found elevated expression of interleukin (IL)-23 and IL-10 in untreated MS patients. IFN-beta therapy increased IL-10 and decreased IL-23 expression independently...... of the regulatory cytokine IL-10. The elevated IL-23 mRNA levels in MS patients are noteworthy in view of the newly discovered IL-23-driven Th17 T-cell subset, which is crucial in animal models of MS. Since IFN-beta therapy resulted in decreased IL-23 mRNA levels, the Th17 axis could be another target of IFN...

  11. Complexity on Acute Myeloid Leukemia mRNA Transcript Variant

    Directory of Open Access Journals (Sweden)

    Carlo Cattani

    2011-01-01

    Full Text Available This paper deals with the sequence analysis of acute myeloid leukemia mRNA. Six transcript variants of mlf1 mRNA, with more than 2000 bps, are analyzed by focusing on the autocorrelation of each distribution. Through the correlation matrix, some patches and similarities are singled out and commented, with respect to similar distributions. The comparison of Kolmogorov fractal dimension will be also given in order to classify the six variants. The existence of a fractal shape, patterns, and symmetries are discussed as well.

  12. Detection of melatonin receptor mRNA in human muscle

    International Nuclear Information System (INIS)

    Li Lei

    2004-01-01

    To verify the expression of melatonin receptor mRNA in human, muscle, muscle beside vertebrae was collected to obtain total RNA and the mRNA of melatonin receptor was detected by RT-PCR method. The electrophoretic results of RT-PCR products by mt 1 and MT 2 primer were all positive and the sequence is corresponding with human melatonin receptor cDNA. It suggests that melatonin may act on the muscle beside vertebrae directly and regulate its growth and development. (authors)

  13. Evaluation of PM-10 commercial inlets for new surveillance air sampler

    International Nuclear Information System (INIS)

    Langer, G.

    1987-01-01

    The purpose of this project is to adapt an existing sampling inlet or develop a new one to collect airborne dust particles <10-μm aerodynamic equivalent diameter. These inlets are necessary to meet new EPA and DOE guidelines for surveillance of nuclear facilities

  14. The installation and performance test of the surveillance system for DUPIC facility

    International Nuclear Information System (INIS)

    Kim, Dong Young; Kim, Ho Dong; Cha, Hong Ryul

    2000-07-01

    We have developed the real time surveillance system, named by DSSS, for DUPIC test facility. The system acquires data from He-3 neutron monitors(DSNM) and CCD cameras to automatically diagnose the transportation status of nuclear material. This technical report shortly illustrates important features of hardware and software of the system

  15. The installation and performance test of the surveillance system for DUPIC facility

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Dong Young; Kim, Ho Dong; Cha, Hong Ryul

    2000-07-01

    We have developed the real time surveillance system, named by DSSS, for DUPIC test facility. The system acquires data from He-3 neutron monitors(DSNM) and CCD cameras to automatically diagnose the transportation status of nuclear material. This technical report shortly illustrates important features of hardware and software of the system.

  16. Expert system for surveillance and diagnosis of breach fuel elements

    Science.gov (United States)

    Gross, Kenny C.

    1989-01-01

    An apparatus and method are disclosed for surveillance and diagnosis of breached fuel elements in a nuclear reactor. A delayed neutron monitoring system provides output signals indicating the delayed neutron activity and age and the equivalent recoil areas of a breached fuel element. Sensors are used to provide outputs indicating the status of each component of the delayed neutron monitoring system. Detectors also generate output signals indicating the reactor power level and the primary coolant flow rate of the reactor. The outputs from the detectors and sensors are interfaced with an artificial intelligence-based knowledge system which implements predetermined logic and generates output signals indicating the operability of the reactor.

  17. Expert system for surveillance and diagnosis of breach fuel elements

    International Nuclear Information System (INIS)

    Gross, K.C.

    1989-01-01

    An apparatus and method are disclosed for surveillance and diagnosis of breached fuel elements in a nuclear reactor. A delayed neutron monitoring system provides output signals indicating the delayed neutron activity and age and the equivalent recoil areas of a breached fuel element. Sensors are used to provide outputs indicating the status of each component of the delayed neutron monitoring system. Detectors also generate output signals indicating the reactor power level and the primary coolant flow rate of the reactor. The outputs from the detectors and sensors are interfaced with an artificial intelligence-based knowledge system which implements predetermined logic and generates output signals indicating the operability of the reactor

  18. Radiation surveillance procedure during veterinary application of radioisotope

    International Nuclear Information System (INIS)

    Kamaldeep; Bhaktivinayagam, A.; Singh, Sanjay Kumar

    2012-01-01

    Radioisotopes have found wide applications in the field of biomedical veterinary nuclear medicine and research. Radiation safety issues during internal administration of radioisotopes to laboratory animals, unlike human use, are far more challenging and requires stringent, well planned and an organized system of radiation protection in the animal house facility. In this paper, we discuss our experience during veterinary research experiments involving use, handling and administration of liquid sources of 131 I. With extensive radiation protection surveillance and application of practical and essential radiation safety and hygiene practices, the radiation exposure and contamination levels during the veterinary application of isotopes can be kept ALARA

  19. Laboratory of environmental radiological surveillance; Laboratorio de vigilancia radiologica ambiental

    Energy Technology Data Exchange (ETDEWEB)

    Mendez G, A.; Marcial M, F.; Giber F, J.; Montiel R, E.; Leon del V, E.; Rivas C, I.; Leon G, M.V.; Lagunas G, E.; Aragon S, R.; Juarez N, A.; Alfaro L, M.M

    1991-12-15

    The department of radiological protection of the ININ requests the collaboration of the Engineering Unit for the elaboration of the work project of the laboratory of environmental radiological surveillance. The emission of radioactive substances to the atmosphere like consequence of the normal operation of the Nuclear Center, constitutes an exhibition source from the man to the radiations that it should be appropriately watched over and controlled to be able to determine the population's potential exhibition that it lives in the area of influence of the installation. (Author)

  20. Application of digital compression techniques to optical surveillance systems

    International Nuclear Information System (INIS)

    Johnson, C.S.

    1991-01-01

    There are many benefits to handling video images electronically, however, the amount of digital data in a normal video image is a major obstacle. The solution is to remove the high frequency and redundant information in a process that is referred to as compression. Compression allows the number of digital bits required for a given image to be reduced for more efficient storage or transmission of images. The next question is how much compression can be done without impairing the image quality beyond its usefulness for a given application. This paper discusses image compression that might be applied to provide useful images in unattended nuclear facility surveillance applications

  1. Laboratory of environmental radiological surveillance; Laboratorio de vigilancia radiologica ambiental

    Energy Technology Data Exchange (ETDEWEB)

    Mendez G, A; Marcial M, F; Giber F, J; Montiel R, E; Leon del V, E; Rivas C, I; Leon G, M V; Lagunas G, E; Aragon S, R; Juarez N, A; Alfaro L, M M

    1991-12-15

    The department of radiological protection of the ININ requests the collaboration of the Engineering Unit for the elaboration of the work project of the laboratory of environmental radiological surveillance. The emission of radioactive substances to the atmosphere like consequence of the normal operation of the Nuclear Center, constitutes an exhibition source from the man to the radiations that it should be appropriately watched over and controlled to be able to determine the population's potential exhibition that it lives in the area of influence of the installation. (Author)

  2. Reevaluation of air surveillance station siting

    International Nuclear Information System (INIS)

    Abbott, K.; Jannik, T.

    2016-01-01

    DOE Technical Standard HDBK-1216-2015 (DOE 2015) recommends evaluating air-monitoring station placement using the analytical method developed by Waite. The technique utilizes wind rose and population distribution data in order to determine a weighting factor for each directional sector surrounding a nuclear facility. Based on the available resources (number of stations) and a scaling factor, this weighting factor is used to determine the number of stations recommended to be placed in each sector considered. An assessment utilizing this method was performed in 2003 to evaluate the effectiveness of the existing SRS air-monitoring program. The resulting recommended distribution of air-monitoring stations was then compared to that of the existing site perimeter surveillance program. The assessment demonstrated that the distribution of air-monitoring stations at the time generally agreed with the results obtained using the Waite method; however, at the time new stations were established in Barnwell and in Williston in order to meet requirements of DOE guidance document EH-0173T.

  3. Reevaluation of air surveillance station siting

    Energy Technology Data Exchange (ETDEWEB)

    Abbott, K. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Jannik, T. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

    2016-07-06

    DOE Technical Standard HDBK-1216-2015 (DOE 2015) recommends evaluating air-monitoring station placement using the analytical method developed by Waite. The technique utilizes wind rose and population distribution data in order to determine a weighting factor for each directional sector surrounding a nuclear facility. Based on the available resources (number of stations) and a scaling factor, this weighting factor is used to determine the number of stations recommended to be placed in each sector considered. An assessment utilizing this method was performed in 2003 to evaluate the effectiveness of the existing SRS air-monitoring program. The resulting recommended distribution of air-monitoring stations was then compared to that of the existing site perimeter surveillance program. The assessment demonstrated that the distribution of air-monitoring stations at the time generally agreed with the results obtained using the Waite method; however, at the time new stations were established in Barnwell and in Williston in order to meet requirements of DOE guidance document EH-0173T.

  4. Surveillance and threat detection prevention versus mitigation

    CERN Document Server

    Kirchner, Richard

    2014-01-01

    Surveillance and Threat Detection offers readers a complete understanding of the terrorist/criminal cycle, and how to interrupt that cycle to prevent an attack. Terrorists and criminals often rely on pre-attack and pre-operational planning and surveillance activities that can last a period of weeks, months, or even years. Identifying and disrupting this surveillance is key to prevention of attacks. The systematic capture of suspicious events and the correlation of those events can reveal terrorist or criminal surveillance, allowing security professionals to employ appropriate countermeasures and identify the steps needed to apprehend the perpetrators. The results will dramatically increase the probability of prevention while streamlining protection assets and costs. Readers of Surveillance and Threat Detection will draw from real-world case studies that apply to their real-world security responsibilities. Ultimately, readers will come away with an understanding of how surveillance detection at a high-value, f...

  5. Health effects and medical surveillance

    International Nuclear Information System (INIS)

    2004-01-01

    This Practical Radiation Technical Manual is one of a series which has been designed to provide guidance on radiological protection for employers, Radiation Protection Officers, managers and other technically competent persons who have a responsibility to ensure the safety of employees working with ionizing radiation. The Manual may be used with the appropriate IAEA Practical Radiation Safety Manuals to provide adequate training, instruction or information on health effects and medical surveillance for all employees engaged in work with ionizing radiation. Sources of ionizing radiations have a large number of applications in the workplace. Usually, even where the work is performed safely, the employees involved inevitably receive small, regular exposures to radiation that are not harmful. Some applications involve sources that could deliver more significant radiation doses, particularly when poor methods are practised or an accident occurs. The radiations cannot be seen, felt or sensed by the human body in any way and excessive exposures may cause detriment to the health of a worker in a way that is not immediately apparent. When the symptoms occur, weeks or possibly years later, an untrained worker or inexperienced medical staff probably cannot recognize the effects to be due to the radiation exposure. This Manual explains how ionizing radiations can interact with and affect human tissues, the various factors that influence the outcome and the detrimental effects that may result. The medical surveillance that is appropriate for those working with radiation sources, depending on the degree of hazard of the work, is described. The Manual will be of most benefit if it forms part of more comprehensive training or is supplemented by the advice of a medically qualified expert. Where medical surveillance is appropriate for radiation employees, the services of a qualified doctor, occupational physician or other trained medical staff will be required

  6. Somatic surveillance: corporeal control through information networks

    OpenAIRE

    Monahan, Torin; Wall, Tyler

    2007-01-01

    Somatic surveillance is the increasingly invasive technological monitoring of and intervention into body functions. Within this type of surveillance regime, bodies are recast as nodes on vast information networks, enabling corporeal control through remote network commands, automated responses, or self-management practices. In this paper, we investigate three developments in somatic surveillance: nanotechnology systems for soldiers on the battlefield, commercial body-monitoring systems for hea...

  7. Surveillance of WWER-440 fuel performance

    International Nuclear Information System (INIS)

    Simko, J.; Urban, P.

    1999-01-01

    In this lecture next problems of surveillance of WWER-440 fuel performance are presented: surveillance of WWER-440 fuel performance at Mochovce NPP; basic data of WWER-440 reactor; in-core reactor measuring system 'SVRK'; basic level of SVRK; information output of basic level of SVRK; surveillance of fuel performance; table of permissible operation conditions of the reactor; limitation of the unit 1 power at the beginning of the operation; cyclic changes of power; future perspectives

  8. The relaxation of ESFAS/RPS surveillance test requirements

    International Nuclear Information System (INIS)

    Hah, Yung Joon; Koo, Jung Eui; Choi, Hae Yoon

    1994-01-01

    The surveillance test requirement of ESFAS/RPS is reviewed for 950 MWe class westinghouse reactor (YGN unit 1 and 2, Kori unit 3 and 4). The current requirements of frequent test and maintenance in the tech. spec. can lead to human errors, jeopardizing safety of the plant, and reduction in the availability of the plant. Meanwhile, the ESFAS designs do not provide for complete online testing capabilities for their protection systems. Therefore, ESFAS slave relays cannot be tested during plant operation as actuation of associated equipment could result in unwanted plant transient or trip conditions. In this study, westinghouse's PSA results, NRC recommendation and NRC approval status for specific U.S. nuclear power plant have been reviewed and evaluated. Since YGN 1 and 2 and Kori 3 and 4 are essentially the same plant as the operating westinghouse plant in the U.S., it is expected that YGN 1 and 2 and Kori 3 and 4 will be justified for having ESFAS/RPS surveillance test requirements relaxation program. Finally the extension of surveillance testing intervals and allowed outage times for test and maintenance will be verified by PSA program for YGN 1 and 2 and Kori 3 and 4. Various findings during the project can be used in the possible future revision of current technical specification with further refinements through the PSA program for YGN 1 and 2 and Kori 3 and 4. (Author) 10 refs., 8 figs., 24 tabs

  9. The relaxation of ESFAS/RPS surveillance test requirements

    Energy Technology Data Exchange (ETDEWEB)

    Hah, Yung Joon; Koo, Jung Eui; Choi, Hae Yoon [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1994-01-01

    The surveillance test requirement of ESFAS/RPS is reviewed for 950 MWe class westinghouse reactor (YGN unit 1 and 2, Kori unit 3 and 4). The current requirements of frequent test and maintenance in the tech. spec. can lead to human errors, jeopardizing safety of the plant, and reduction in the availability of the plant. Meanwhile, the ESFAS designs do not provide for complete online testing capabilities for their protection systems. Therefore, ESFAS slave relays cannot be tested during plant operation as actuation of associated equipment could result in unwanted plant transient or trip conditions. In this study, westinghouse`s PSA results, NRC recommendation and NRC approval status for specific U.S. nuclear power plant have been reviewed and evaluated. Since YGN 1 and 2 and Kori 3 and 4 are essentially the same plant as the operating westinghouse plant in the U.S., it is expected that YGN 1 and 2 and Kori 3 and 4 will be justified for having ESFAS/RPS surveillance test requirements relaxation program. Finally the extension of surveillance testing intervals and allowed outage times for test and maintenance will be verified by PSA program for YGN 1 and 2 and Kori 3 and 4. Various findings during the project can be used in the possible future revision of current technical specification with further refinements through the PSA program for YGN 1 and 2 and Kori 3 and 4. (Author) 10 refs., 8 figs., 24 tabs.

  10. Problems with radiological surveillance instrumentation

    International Nuclear Information System (INIS)

    Swinth, K.L.; Tanner, J.E.; Fleming, D.M.

    1984-09-01

    Many radiological surveillance instruments are in use at DOE facilities throughout the country. These instruments are an essential part of all health physics programs, and poor instrument performance can increase program costs or compromise program effectiveness. Generic data from simple tests on newly purchased instruments shows that many instruments will not meet requirements due to manufacturing defects. In other cases, lack of consideration of instrument use has resulted in poor acceptance of instruments and poor reliability. The performance of instruments is highly variable for electronic and mechanical performance, radiation response, susceptibility to interferences and response to environmental factors. Poor instrument performance in these areas can lead to errors or poor accuracy in measurements

  11. Problems with radiological surveillance instrumentation

    International Nuclear Information System (INIS)

    Swinth, K.L.; Tanner, J.E.; Fleming, D.M.

    1985-01-01

    Many radiological surveillance instruments are in use at DOE facilities throughout the country. These instruments are an essential part of all health physics programs, and poor instrument performance can increase program costs or compromise program effectiveness. Generic data from simple tests on newly purchased instruments shows that many instruments will not meet requirements due to manufacturing defects. In other cases, lack of consideration of instrument use has resulted in poor acceptance of instruments and poor reliability. The performance of instruments is highly variable for electronic and mechanical performance, radiation response, susceptibility to interferences and response to environmental factors. Poor instrument performance in these areas can lead to errors or poor accuracy in measurements

  12. Legionnaires’ disease Surveillance in Italy

    Directory of Open Access Journals (Sweden)

    Maria Luisa Ricci

    2004-12-01

    Full Text Available

    In the report presented, data on legionellosis diagnosed in the year 2003 in Italy and notified to the National Surveillance System are analysed. Overall, 617 cases were notified, of which 517 were confirmed and 46 were presumptive.

    The characteristics of the patients are very similar to those reported in the previous years in terms of male/female ratio, age–specific distribution, occupation, etc. Legionella pneumophila serogroup 1 was responsible for approximately 90% of the cases.

  13. Next generation surveillance system (NGSS)

    International Nuclear Information System (INIS)

    Aparo, Massimo

    2006-01-01

    Development of 'functional requirements' for transparency systems may offer a near-term mode of regional cooperation. New requirements under development at the IAEA may provide a foundation for this potential activity. The Next Generation Surveillance System (NGSS) will become the new IAEA remote monitoring system Under new requirements the NGSS would attempt to use more commercial components to reduce cost, increase radiation survivability and further increase reliability. The NGSS must be available in two years due to rapidly approaching obsolescence in the existing DCM family. (author)

  14. Reactor surveillance by noise analysis

    International Nuclear Information System (INIS)

    Ciftcioglu, Ozer

    1988-01-01

    A real-time noise analysis system is designed for the TRIGA reactor at Istanbul Technical University. By means of the noise techniques, reactor surveillance is performed together with failure diagnosis. The fast data processing is carried out by FFT in real-time so that malfunction or non-stationary operation of the reactor in long term can be identified by comparing the noise power spectra with the corresponding reference patterns while the decision making procedure is accomplished by the method of hypothesis testing. The system being computer based safety instrumentation involves CAMAC in conjunction with the RT-11 (PDP-11) single user dedicated environment. (author)

  15. Cloning and mRNA expression pattern analysis under low ...

    African Journals Online (AJOL)

    This research cloned endochitinase-antifreeze protein precursor (EAPP) gene of Dong-mu 70 rye (Secale cereale) by designing special primers according to Genbank's EAPP gene sequence, and analyzing the influence of low temperature stress on the expression of mRNA with RT-PCR. The results indicated that the ...

  16. Tools to identify the men with prostate cancer most appropriate for active surveillance?

    Directory of Open Access Journals (Sweden)

    Robert H Getzenberg

    2014-02-01

    Full Text Available A great deal of effort is underway in order to identify those men with prostate cancer felicitous for active surveillance with greater precision than that afforded to us today. In the manuscript by Irshad et al. the authors evaluate a novel set of genes associated with senescence and aging as tools that can provide guidance regarding the indolent nature of an individual's prostate cancer with validation using both mRNA and protein analyses. While additional studies are required to understand the full impact of these findings, the innovative approach taken enhances our understanding of distinct phenotypes of prostate cancer.

  17. Negotiating privacy in surveillant welfare relations

    DEFF Research Database (Denmark)

    Andersen, Lars Bo; Lauritsen, Peter; Bøge, Ask Risom

    . However, while privacy is central to debates of surveillance, it has proven less productive as an analytical resource for studying surveillance in practice. Consequently, this paper reviews different conceptualisations of privacy in relation to welfare and surveillance and argues for strengthening...... the analytical capacity of the concept by rendering it a situated and relational concept. The argument is developed through a research and design project called Teledialogue meant to improve the relation between case managers and children placed at institutions or in foster families. Privacy in Teledialogue...... notion of privacy are discussed in relation to both research- and public debates on surveillance in a welfare setting....

  18. Multi-Camera Persistent Surveillance Test Bed

    National Research Council Canada - National Science Library

    Baran, David; O'Brien, Barry; Fung, Nick; Kovach, Jesse; Miller, David

    2007-01-01

    .... Small reconnaissance surveillance and target acquisition (RSTA) platforms have the ability to cooperate through information sharing to increase the situational awareness over a region of interest (RSTA...

  19. Statins Activate Human PPAR Promoter and Increase PPAR mRNA Expression and Activation in HepG2 Cells

    Directory of Open Access Journals (Sweden)

    Makoto Seo

    2008-01-01

    Full Text Available Statins increase peroxisome proliferator-activated receptor (PPAR mRNA expression, but the mechanism of this increased PPAR production remains elusive. To examine the regulation of PPAR production, we examined the effect of 7 statins (atorvastatin, cerivastatin, fluvastatin, pitavastatin, pravastatin, rosuvastatin, and simvastatin on human PPAR promoter activity, mRNA expression, nuclear protein levels, and transcriptional activity. The main results are as follows. (1 Majority of statins enhanced PPAR promoter activity in a dose-dependent manner in HepG2 cells transfected with the human PPAR promoter. This enhancement may be mediated by statin-induced HNF-4. (2 PPAR mRNA expression was increased by statin treatment. (3 The PPAR levels in nuclear fractions were increased by statin treatment. (4 Simvastatin, pravastatin, and cerivastatin markedly enhanced transcriptional activity in 293T cells cotransfected with acyl-coenzyme A oxidase promoter and PPAR/RXR expression vectors. In summary, these data demonstrate that PPAR production and activation are upregulated through the PPAR promoter activity by statin treatment.

  20. Los Alamos National Laboratory SAVY-4000 Field Surveillance Plan Update for 2016

    International Nuclear Information System (INIS)

    Kelly, Elizabeth J.; Stone, Timothy Amos; Smith, Paul Herrick; Prochnow, David Adrian; Weis, Eric M.

    2016-01-01

    The Packaging Surveillance Program section of the Department of Energy (DOE) Manual 441.1-1, Nuclear Material Packaging Manual (DOE 2008), requires DOE contractors to ''ensure that a surveillance program is established and implemented to ensure the nuclear material storage package continues to meet its design criteria.'' The Los Alamos National Laboratory (LANL) SAVY-4000 Field Surveillance Plan was first issued in FY 2013 (Kelly et al. 2013). The surveillance plan is reviewed annually and updated as necessary based on SAVY-4000 surveillance and other surveillance findings, as well as results of the lifetime extension studies (Blair et al. 2012, Weis et al. 2015a). The LANL SAVY-4000 Field Surveillance Plan Update was issued in 2014 (Kelly et al. 2014). This 2016 update reflects changes to the surveillance plan resulting from restrictions on handling residue materials greater than 500 g, the addition of specific engineering judgment containers, and 2015 surveillance findings. The SAVY-4000 container has a design life of five years, which was chosen as a conservative estimate of the functional properties of the materials used in the construction of the SAVY 4000 when exposed to the potential insults including temperature, corrosive materials and gases, and radiation. The SAVY-4000 container design basis is described in a safety analysis report (Anderson et al. 2013). In the National Nuclear Security Administration's (NNSA's) approval of the safety analysis report, it was recommended that the design life clock begin on March 2014 (Nez et al. 2014). However, it is expected that a technical basis can be developed to extend the design life of the SAVY-4000 containers to approximately 40 years (Blair et al. 2012, Weis et al. 2015a). This surveillance plan update covers five years (2015-2019) and is developed to ensure SAVY-4000 containers meet their design criteria over the current five-year design life and to gather data that can be used in

  1. Environmental management in nuclear industry

    International Nuclear Information System (INIS)

    Pillai, K.C.; Bhat, I.S.

    1988-01-01

    Safety of the environment is given due attention right at the design state of nuclear energy installations. Besides this engineered safety environmental protection measures are taken on (a) site selection criteria (b) waste management practices (c) prescribing dose limits for the public (d) having intensive environmental surveillance programme and (e) emergency preparedness. The paper enumerates the application of these protection measures in the environmental management to make the nuclear industry as an example to follow in the goal of environmental safety. (author)

  2. Using Acute Flaccid Paralysis Surveillance as a Platform for Vaccine-Preventable Disease Surveillance.

    Science.gov (United States)

    Wassilak, Steven G F; Williams, Cheryl L; Murrill, Christopher S; Dahl, Benjamin A; Ohuabunwo, Chima; Tangermann, Rudolf H

    2017-07-01

    Surveillance for acute flaccid paralysis (AFP) is a fundamental cornerstone of the global polio eradication initiative (GPEI). Active surveillance (with visits to health facilities) is a critical strategy of AFP surveillance systems for highly sensitive and timely detection of cases. Because of the extensive resources devoted to AFP surveillance, multiple opportunities exist for additional diseases to be added using GPEI assets, particularly because there is generally 1 district officer responsible for all disease surveillance. For this reason, integrated surveillance has become a standard practice in many countries, ranging from adding surveillance for measles and rubella to integrated disease surveillance for outbreak-prone diseases (integrated disease surveillance and response). This report outlines the current level of disease surveillance integration in 3 countries (Nepal, India, and Nigeria) and proposes that resources continue for long-term maintenance in resource-poor countries of AFP surveillance as a platform for surveillance of vaccine-preventable diseases and other outbreak-prone diseases. © The Author 2017. Published by Oxford University Press for the Infectious Diseases Society of America.

  3. Limits on surveillance: frictions, fragilities and failures in the operation of camera surveillance.

    NARCIS (Netherlands)

    Dubbeld, L.

    2004-01-01

    Public video surveillance tends to be discussed in either utopian or dystopian terms: proponents maintain that camera surveillance is the perfect tool in the fight against crime, while critics argue that the use of security cameras is central to the development of a panoptic, Orwellian surveillance

  4. Cyber Surveillance for Flood Disasters

    Directory of Open Access Journals (Sweden)

    Shi-Wei Lo

    2015-01-01

    Full Text Available Regional heavy rainfall is usually caused by the influence of extreme weather conditions. Instant heavy rainfall often results in the flooding of rivers and the neighboring low-lying areas, which is responsible for a large number of casualties and considerable property loss. The existing precipitation forecast systems mostly focus on the analysis and forecast of large-scale areas but do not provide precise instant automatic monitoring and alert feedback for individual river areas and sections. Therefore, in this paper, we propose an easy method to automatically monitor the flood object of a specific area, based on the currently widely used remote cyber surveillance systems and image processing methods, in order to obtain instant flooding and waterlogging event feedback. The intrusion detection mode of these surveillance systems is used in this study, wherein a flood is considered a possible invasion object. Through the detection and verification of flood objects, automatic flood risk-level monitoring of specific individual river segments, as well as the automatic urban inundation detection, has become possible. The proposed method can better meet the practical needs of disaster prevention than the method of large-area forecasting. It also has several other advantages, such as flexibility in location selection, no requirement of a standard water-level ruler, and a relatively large field of view, when compared with the traditional water-level measurements using video screens. The results can offer prompt reference for appropriate disaster warning actions in small areas, making them more accurate and effective.

  5. Video surveillance using distance maps

    Science.gov (United States)

    Schouten, Theo E.; Kuppens, Harco C.; van den Broek, Egon L.

    2006-02-01

    Human vigilance is limited; hence, automatic motion and distance detection is one of the central issues in video surveillance. Hereby, many aspects are of importance, this paper specially addresses: efficiency, achieving real-time performance, accuracy, and robustness against various noise factors. To obtain fully controlled test environments, an artificial development center for robot navigation is introduced in which several parameters can be set (e.g., number of objects, trajectories and type and amount of noise). In the videos, for each following frame, movement of stationary objects is detected and pixels of moving objects are located from which moving objects are identified in a robust way. An Exact Euclidean Distance Map (E2DM) is utilized to determine accurately the distances between moving and stationary objects. Together with the determined distances between moving objects and the detected movement of stationary objects, this provides the input for detecting unwanted situations in the scene. Further, each intelligent object (e.g., a robot), is provided with its E2DM, allowing the object to plan its course of action. Timing results are specified for each program block of the processing chain for 20 different setups. So, the current paper presents extensive, experimentally controlled research on real-time, accurate, and robust motion detection for video surveillance, using E2DMs, which makes it a unique approach.

  6. Hanford Site surface environmental surveillance

    International Nuclear Information System (INIS)

    Dirkes, R.L.

    1998-01-01

    Environmental surveillance of the Hanford Site and the surrounding region is conducted to demonstrate compliance with environmental regulations, confirm adherence to US Department of Energy (DOE) environmental protection policies, support DOE environmental management decisions, and provide information to the public. The Surface Environmental Surveillance Project (SESP) is a multimedia environmental monitoring program conducted to measure the concentrations of radionuclides and chemical contaminants in the environment and assess the integrated effects of these contaminants on the environment and the public. The monitoring program includes sampling air, surface water, sediments, soil, natural vegetation, agricultural products, fish, and wildlife. Functional elements inherent in the operation of the SESP include project management, quality assurance/control, training, records management, environmental sampling network design and implementation, sample collection, sample analysis, data management, data review and evaluation, exposure assessment, and reporting. The SESP focuses on those contaminant/media combinations calculated to have the highest potential for contributing to off-site exposure. Results of the SESP indicate that contaminant concentrations in the Hanford environs are very low, generally below environmental standards, at or below analytical detection levels, and indicative of environmental levels. However, areas of elevated contaminant concentrations have been identified at Hanford. The extent of these areas is generally limited to past operating areas and waste disposal sites

  7. Radioactivity surveillance in Peruvian fishmeal

    International Nuclear Information System (INIS)

    Lopez, Edith; Osores, Jose; Gonzales, Susana; Martinez, Jorge; Jara, Raul

    2008-01-01

    Full text: Fishmeal is a derived product of fish which is widely used to feed livestock. It is the brown flour obtained after cooking, pressing, drying and milling whole fish and food fish trimmings. Use of whole fish is almost exclusively from small, bony species of pelagic fish (generally living in the surface waters or middle depths of the sea), for which there is little or no demand for human consumption. In many cases, it constitutes the main source of protein in the diet of livestock. Traditionally, Peru has been a producer and exporter country of fish and its derived products. It is considered one of the top producers of fish worldwide. In Peru, anchovy (Engraulis ringens) is by far the most important species for fishmeal production. As part of the Peruvian national program of environmental surveillance, samples of fishmeal taken from different places of sampling (plants of production located in the northern coast of Peru) were measured and analyzed by HpGe gamma spectrometry. This study shows the results of radioactivity surveillance in Peruvian fishmeal, focusing in the contents of 137 Cs, which indicates that the levels of this radionuclide in the samples are below the order of the minimum detectable concentration (Bq/kg). These results are consistent with those obtained by the UK Food Standards Agency in 1999. According to many international regulations, the level of 137 Cs in foodstuff must be below 600 Bq/kg. (author)

  8. Environmental surveillance master sampling schedule

    International Nuclear Information System (INIS)

    Bisping, L.E.

    1991-01-01

    Environmental surveillance of the Hanford Site and surrounding areas is conducted by the Pacific Northwest Laboratory (PNL) for the US Department of Energy (DOE). This document contains the planned schedule for routine sample collection for the Surface Environmental Surveillance Project (SESP) and Ground-Water Monitoring Project. The routine sampling plan for the SESP has been revised this year to reflect changing site operations and priorities. Some sampling previously performed at least annually has been reduced in frequency, and some new sampling to be performed at a less than annual frequency has been added. Therefore, the SESP schedule reflects sampling to be conducted in calendar year 1991 as well as future years. The ground-water sampling schedule is for 1991. This schedule is subject to modification during the year in response to changes in Site operation, program requirements, and the nature of the observed results. Operational limitations such as weather, mechanical failures, sample availability, etc., may also require schedule modifications. Changes will be documented in the respective project files, but this plan will not be reissued. The purpose of these monitoring projects is to evaluate levels of radioactive and nonradioactive pollutants in the Hanford evirons

  9. Environmental surveillance master sampling schedule

    Energy Technology Data Exchange (ETDEWEB)

    Bisping, L.E.

    1991-01-01

    Environmental surveillance of the Hanford Site and surrounding areas is conducted by the Pacific Northwest Laboratory (PNL) for the US Department of Energy (DOE). This document contains the planned schedule for routine sample collection for the Surface Environmental Surveillance Project (SESP) and Ground-Water Monitoring Project. The routine sampling plan for the SESP has been revised this year to reflect changing site operations and priorities. Some sampling previously performed at least annually has been reduced in frequency, and some new sampling to be performed at a less than annual frequency has been added. Therefore, the SESP schedule reflects sampling to be conducted in calendar year 1991 as well as future years. The ground-water sampling schedule is for 1991. This schedule is subject to modification during the year in response to changes in Site operation, program requirements, and the nature of the observed results. Operational limitations such as weather, mechanical failures, sample availability, etc., may also require schedule modifications. Changes will be documented in the respective project files, but this plan will not be reissued. The purpose of these monitoring projects is to evaluate levels of radioactive and nonradioactive pollutants in the Hanford evirons.

  10. Surveillance as a complement to irradiation embrittlement studies: Status and needs

    International Nuclear Information System (INIS)

    Steele, L.E.

    1977-01-01

    The history of the study of radiation embrittlement of reactor pressure vessel steels has gone through three stages in the USA. 1) A scientific curiosity. 2) Empirical or laboratory evaluation of typical steels, and 3) Integration of the scientific and empirical to advance status and evolve standard techniques. The current stage is one in which surveillance data compliments the laboratory studies which characterized Stage 3. The early USA surveillance programs were generally analyzed by the same people who were the primary laboratory investigators. An effort must be made to continue this type of collaboration as a useful two-way learning procedure though it will become more and more difficult as nuclear power is broadly commercialized. The current status of both types of USA programs will be presented to encourage the most advantageous use of data from both sources. At this time about 25 USA nuclear power reactors have operated long enough to have provided initial surveillance or dosimetry results. An effort will be made to summarize the general status of these in order to: 1) Provide complimentary data to laboratory studies. 2) Assess directions in handling the problems of radiation embrittlement. 3) Note lessons learned for improving surveillance efforts in the future. 4) Identify possible research tasks for the future to support in-service surveillance and other measures. 5) Justify facts advancing surveillance requirements to status of national codes and standards. 6) Justify facts requiring changes in current national codes and standards. A plan will be presented along with an introduction of each member of the USA delegation for systematic presentation of the status of reactor vessel surveillance in the USA. (author)

  11. Materials surveillance program for C-E NSSS reactor vessels

    International Nuclear Information System (INIS)

    Koziol, J.J.

    1977-01-01

    Irradiation surveillance programs for light water NSSS reactor vessels provide the means by which the utility can assess the extent of neutron-induced changes in the reactor vessel materials. These programs are conducted to verify, by direct measurement, the conservatism in the predicted radiation-induced changes and hence the operational parameters (i.e., heat-up, cooldown, and pressurization rates). In addition, such programs provide assurance that the scheduled adjustments in the operational parameters are made with ample margin for safe operation of the plant. During the past 3 years, several documents have been promulgated establishing the criteria for determining both the initial properties of the reactor vessel materials as well as measurement of changes in these initial properties as a result of irradiation. These documents, ASTM E-185-73, ''Recommended Practice for Surveillance Tests for Nuclear Reactor Vessels,'' and Appendix H to 10 CFR 50, ''Reactor Vessel Material Surveillance Program Requirements,'' are complementary to each other. They are the result of a change in the basic philosophy regarding the design and analysis of reactor vessels. In effect, the empirical ''transition temperature approach,'' which was used for design, was replaced by the ''analytical fracture mechanics approach.'' The implementation of this technique was described in Welding Research Council Bulletin 1975 and Appendix G to ASME Code Section III. Further definition of requirements appears in Appendix G to 10 CFR 50 published in July 1973. It is the intent of this paper to describe (1) a typical materials surveillance program for the reactor vessel of a Combustion Engineering NSSS, and (2) how the results of such programs, as well as experimental programs provide feed-back for improvement of materials to enhance their radiation resistance and thereby further improve the safety and reliability of future plants. (author)

  12. The Clothes Make the mRNA: Past and Present Trends in mRNP Fashion.

    Science.gov (United States)

    Singh, Guramrit; Pratt, Gabriel; Yeo, Gene W; Moore, Melissa J

    2015-01-01

    Throughout their lifetimes, messenger RNAs (mRNAs) associate with proteins to form ribonucleoproteins (mRNPs). Since the discovery of the first mRNP component more than 40 years ago, what is known as the mRNA interactome now comprises >1,000 proteins. These proteins bind mRNAs in myriad ways with varying affinities and stoichiometries, with many assembling onto nascent RNAs in a highly ordered process during transcription and precursor mRNA (pre-mRNA) processing. The nonrandom distribution of major mRNP proteins observed in transcriptome-wide studies leads us to propose that mRNPs are organized into three major domains loosely corresponding to 5' untranslated regions (UTRs), open reading frames, and 3' UTRs. Moving from the nucleus to the cytoplasm, mRNPs undergo extensive remodeling as they are first acted upon by the nuclear pore complex and then by the ribosome. When not being actively translated, cytoplasmic mRNPs can assemble into large multi-mRNP assemblies or be permanently disassembled and degraded. In this review, we aim to give the reader a thorough understanding of past and current eukaryotic mRNP research.

  13. HIV-1 matrix dependent membrane targeting is regulated by Gag mRNA trafficking.

    Directory of Open Access Journals (Sweden)

    Jing Jin

    Full Text Available Retroviral Gag polyproteins are necessary and sufficient for virus budding. Productive HIV-1 Gag assembly takes place at the plasma membrane. However, little is known about the mechanisms by which thousands of Gag molecules are targeted to the plasma membrane. Using a bimolecular fluorescence complementation (BiFC assay, we recently reported that the cellular sites and efficiency of HIV-1 Gag assembly depend on the precise pathway of Gag mRNA export from the nucleus, known to be mediated by Rev. Here we describe an assembly deficiency in human cells for HIV Gag whose expression depends on hepatitis B virus (HBV post-transcriptional regulatory element (PRE mediated-mRNA nuclear export. PRE-dependent HIV Gag expressed well in human cells, but assembled with slower kinetics, accumulated intracellularly, and failed to associate with a lipid raft compartment where the wild-type Rev-dependent HIV-1 Gag efficiently assembles. Surprisingly, assembly and budding of PRE-dependent HIV Gag in human cells could be rescued in trans by co-expression of Rev-dependent Gag that provides correct membrane targeting signals, or in cis by replacing HIV matrix (MA with other membrane targeting domains. Taken together, our results demonstrate deficient membrane targeting of PRE-dependent HIV-1 Gag and suggest that HIV MA function is regulated by the trafficking pathway of the encoding mRNA.

  14. Human-centered automation of testing, surveillance and maintenance

    International Nuclear Information System (INIS)

    Bhatt, S.C.; Sun, B.K.H.

    1991-01-01

    Manual surveillance and testing of instrumentation, control and protection systems at nuclear power plants involves system and human errors which can lead to substantial plant down time. Frequent manual testing can also contribute significantly to operation and maintenance cost. Automation technology offers potential for prudent applications at the power plant to reduce testing errors and cost. To help address the testing problems and to harness the benefit of automation application, input from utilities is obtained on suitable automation approaches. This paper includes lessens from successful past experience at a few plants where some island of automation exist. The results are summarized as a set of specifications for semi automatic testing. A human-centered automation methodology is proposed with the guidelines for optimal human/computer division of tasks given. Implementation obstacles for significant changes of testing practices are identified and methods acceptable to nuclear power plants for addressing these obstacles have been suggested

  15. Elements of quality assurance in environmental surveillance

    International Nuclear Information System (INIS)

    Johnson, L.J.

    1975-01-01

    Qualities of an environmental surveillance program requiring control or assurance are reviewed. Requirements of accuracy, reproducibility, sensitivity, acceptability, and time and cost effectiveness are qualities discussed. The controls applicable to sample collection, handling, chemical analysis, measurement and data presentation are identified and discussed as they pertain to environmental monitoring. Quality assurance program recommendations for developing and reporting environmental surveillance data are provided

  16. European surveillance network for influenza in pigs

    NARCIS (Netherlands)

    Simon, Gaëlle; Larsen, Lars E.; Dürrwald, Ralf; Foni, Emanuela; Harder, Timm; Reeth, Van Kristien; Markowska-Daniel, Iwona; Reid, Scott M.; Dan, Adam; Maldonado, Jaime; Huovilainen, Anita; Billinis, Charalambos; Davidson, Irit; Agüero, Montserrat; Vila, Thaïs; Hervé, Séverine; Breum, Solvej Østergaard; Chiapponi, Chiara; Urbaniak, Kinga; Kyriakis, Constantinos S.; Brown, Ian H.; Loeffen, Willie; Meulen, Van der Karen; Schlegel, Michael; Bublot, Michel; Kellam, Paul; Watson, Simon; Lewis, Nicola S.; Pybus, Oliver G.; Webby, Richard; Chen, Hualan; Vincent, Amy L.

    2014-01-01

    Swine influenza causes concern for global veterinary and public health officials. In continuing two previous networks that initiated the surveillance of swine influenza viruses (SIVs) circulating in European pigs between 2001 and 2008, a third European Surveillance Network for Influenza in Pigs

  17. Ambient Surveillance by Probabilistic-Possibilistic Perception

    NARCIS (Netherlands)

    Bittermann, M.S.; Ciftcioglu, O.

    2013-01-01

    A method for quantifying ambient surveillance is presented, which is based on probabilistic-possibilistic perception. The human surveillance of a scene through observing camera sensed images on a monitor is modeled in three steps. First immersion of the observer is simulated by modeling perception

  18. Video Surveillance: Privacy Issues and Legal Compliance

    DEFF Research Database (Denmark)

    Mahmood Rajpoot, Qasim; Jensen, Christian D.

    2015-01-01

    Pervasive usage of video surveillance is rapidly increasing in developed countries. Continuous security threats to public safety demand use of such systems. Contemporary video surveillance systems offer advanced functionalities which threaten the privacy of those recorded in the video. There is a...

  19. Unattended video surveillance systems for international safeguards

    International Nuclear Information System (INIS)

    Johnson, C.S.

    1979-01-01

    The use of unattended video surveillance systems places some unique requirements on the systems and their hardware. The systems have the traditional requirements of video imaging, video storage, and video playback but also have some special requirements such as tamper safing. The technology available to meet these requirements and how it is being applied to unattended video surveillance systems are discussed in this paper

  20. Long-term surveillance plan for the Mexican Hat disposal site Mexican Hat, Utah

    International Nuclear Information System (INIS)

    1997-06-01

    This long-term surveillance plan (LTSP) describes the U.S. Department of Energy's (DOE) long-term care program for the Uranium Mill Tailings Remedial Action (UMTRA) Project Mexican Hat, Utah, disposal site. This LSTP describes the long-term surveillance program the DOE will implement to ensure the Mexican Hat disposal site performs as designed and is cared for in a manner that protects the public health and safety and the environment. Before each disposal site is licensed for custody and long-term care, the Nuclear Regulatory Commission (NRC) requires the DOE to submit such a site-specific LTSP

  1. Long-term surveillance plan for the Mexican Hat disposal site, Mexican Hat, Utah

    International Nuclear Information System (INIS)

    1996-01-01

    This plan describes the long-term surveillance activities for the Uranium Mill Tailings Remedial Action (UMTRA) Project disposal site at Mexican Hat, Utah. The US Department of Energy (DOE) will carry out these activities to ensure that the disposal site continues to function as designed. This long-term surveillance plan (LTSP) was prepared as a requirement for acceptance under the US Nuclear Regulatory Commission (NRC) general license for custody and long-term care of residual radioactive material (RRM). This LTSPC documents the land ownership interests and details how the long-term care of the disposal site will be accomplished

  2. Long-term surveillance plan for the South Clive disposal site Clive, Utah

    International Nuclear Information System (INIS)

    1997-09-01

    This long-term surveillance plan (LTSP) describes the U.S. Department of Energy's (DOE) long-term care program for the Uranium Mill Tailings Remedial Action (UMTRA) Project South Clive disposal site in Clive, Utah. This LSTP describes the long-term surveillance program the DOE will implement to ensure the South Clive disposal site performs as designed and is cared for in a manner that protects the public health and safety and the environment. Before each disposal site is licensed for custody and long-term care, the Nuclear Regulatory Commission (NRC) requires the DOE to submit such a site-specific LTSP

  3. Long-term surveillance plan for the Estes Gulch disposal site near Rifle, Colorado

    International Nuclear Information System (INIS)

    1997-07-01

    This long-term surveillance plan (LTSP) describes the U.S. Department of Energy's (DOE) long-term care program for the Uranium Mill Tailings Remedial Action (UMTRA) Project Estes Gulch disposal site near Rifle, Colorado. This LSTP describes the long-term surveillance program the DOE will implement to ensure the Estes Gulch disposal site performs as designed and is cared for in a manner that protects the public health and safety and the environment. Before each disposal site is licensed for custody and long-term care, the Nuclear Regulatory Commission (NRC) requires the DOE to submit such a site-specific LTSP

  4. Interim long-term surveillance plan for the Cheney disposal site near, Grand Junction, Colorado

    International Nuclear Information System (INIS)

    1997-08-01

    This interim long-term surveillance plan (LTSP) describes the U.S. Department of Energy's (DOE) long-term care program for the Uranium Mill Tailings Remedial Action (UMTRA) Project Cheney Disposal Site in Mesa County near Grand Junction, Colorado. This LSTP describes the long-term surveillance program the DOE will implement to ensure the Cheney disposal site performs as designed and is cared for in a manner that protects the public health and safety and the environment. Before each disposal site is licensed for custody and long-term care, the Nuclear Regulatory Commission (NRC) requires the DOE to submit such a site-specific LTSP

  5. Long-term surveillance plan for the Cheney disposal site near Grand Junction, Colorado

    International Nuclear Information System (INIS)

    1997-07-01

    This long-term surveillance plan (LTSP) describes the U.S. Department of Energy's (DOE) long-term care program for the Uranium Mill Tailings Remedial Action (UMTRA) Project Cheney Disposal Site near Grand Junction, Colorado. This LSTP describes the long-term surveillance program the DOE will implement to ensure the Cheney Disposal Site performs as designed and is cared for in a manner that protects the public health and safety and the environment. Before each disposal site is licensed for custody and long-term care, the Nuclear Regulatory Commission (NRC) requires the DOE to submit such a site-specific LTSP

  6. SCORPIO-VVER core monitoring and surveillance system with advanced capabilities

    International Nuclear Information System (INIS)

    Molnar, J.; Vocka, R.

    2010-01-01

    In this work authors present 12 years of operation experience of core monitoring and surveillance system with advanced capabilities on nuclear power plants on 6 unit of VVER-440 type of reactors at two different NPPs. The original version of the SCORPIO (Surveillance of reactor CORe by PIcture On-line display) system was developed for the western type of PWR reactors. The first version of the SCORPIO-VVER Core Monitoring System for Dukovany NPP (VVER-440 type of reactor, Czech Republic) was developed in 1998. For SCORPIO-VVER implementation at Bohunice NPP in Slovakia (2001) the system was enhanced with startup module KRITEX.

  7. The Vasa Homolog RDE-12 engages target mRNA and multiple argonaute proteins to promote RNAi in C. elegans.

    Science.gov (United States)

    Shirayama, Masaki; Stanney, William; Gu, Weifeng; Seth, Meetu; Mello, Craig C

    2014-04-14

    Argonaute (AGO) proteins are key nuclease effectors of RNAi. Although purified AGOs can mediate a single round of target RNA cleavage in vitro, accessory factors are required for small interfering RNA (siRNA) loading and to achieve multiple-target turnover. To identify AGO cofactors, we immunoprecipitated the C. elegans AGO WAGO-1, which engages amplified small RNAs during RNAi. These studies identified a robust association between WAGO-1 and a conserved Vasa ATPase-related protein RDE-12. rde-12 mutants are deficient in RNAi, including viral suppression, and fail to produce amplified secondary siRNAs and certain endogenous siRNAs (endo-siRNAs). RDE-12 colocalizes with WAGO-1 in germline P granules and in cytoplasmic and perinuclear foci in somatic cells. These findings and our genetic studies suggest that RDE-12 is first recruited to target mRNA by upstream AGOs (RDE-1 and ERGO-1), where it promotes small RNA amplification and/or WAGO-1 loading. Downstream of these events, RDE-12 forms an RNase-resistant (target mRNA-independent) complex with WAGO-1 and may thus have additional functions in target mRNA surveillance and silencing. Copyright © 2014 Elsevier Ltd. All rights reserved.

  8. Surveillance as public matter : Revisiting sousveillance through devices and leaks

    NARCIS (Netherlands)

    van der Velden, L.C.

    2018-01-01

    Our conceptual understanding of surveillance is continuously challenged by digital innovations. Projects that render (digital) surveillance visible and knowable become interesting analytic starting points. Since surveillance consists of technical and often secret processes, this ‘rendering visible’

  9. Microbiological Food Safety Surveillance in China

    Directory of Open Access Journals (Sweden)

    Xiaoyan Pei

    2015-08-01

    Full Text Available Microbiological food safety surveillance is a system that collects data regarding food contamination by foodborne pathogens, parasites, viruses, and other harmful microbiological factors. It helps to understand the spectrum of food safety, timely detect food safety hazards, and provide relevant data for food safety supervision, risk assessment, and standards-setting. The study discusses the microbiological surveillance of food safety in China, and introduces the policies and history of the national microbiological surveillance system. In addition, the function and duties of different organizations and institutions are provided in this work, as well as the generation and content of the surveillance plan, quality control, database, and achievement of the microbiological surveillance of food safety in China.

  10. Priorities for antibiotic resistance surveillance in Europe

    DEFF Research Database (Denmark)

    Fluit, A. C.; van der Bruggen, J. T.; Aarestrup, Frank Møller

    2006-01-01

    Antibiotic resistance is an increasing global problem. Surveillance studies are needed to monitor resistance development, to guide local empirical therapy, and to implement timely and adequate countermeasures. To achieve this, surveillance studies must have standardised methodologies, be longitud......Antibiotic resistance is an increasing global problem. Surveillance studies are needed to monitor resistance development, to guide local empirical therapy, and to implement timely and adequate countermeasures. To achieve this, surveillance studies must have standardised methodologies...... to the various reservoirs of antibiotic-resistant bacteria, such as hospitalised patients, nursing homes, the community, animals and food. Two studies that could serve as examples of tailored programmes are the European Antimicrobial Resistance Surveillance System (EARSS), which collects resistance data during...... of antibiotic resistance....

  11. Intelligent agents for adaptive security market surveillance

    Science.gov (United States)

    Chen, Kun; Li, Xin; Xu, Baoxun; Yan, Jiaqi; Wang, Huaiqing

    2017-05-01

    Market surveillance systems have increasingly gained in usage for monitoring trading activities in stock markets to maintain market integrity. Existing systems primarily focus on the numerical analysis of market activity data and generally ignore textual information. To fulfil the requirements of information-based surveillance, a multi-agent-based architecture that uses agent intercommunication and incremental learning mechanisms is proposed to provide a flexible and adaptive inspection process. A prototype system is implemented using the techniques of text mining and rule-based reasoning, among others. Based on experiments in the scalping surveillance scenario, the system can identify target information evidence up to 87.50% of the time and automatically identify 70.59% of cases depending on the constraints on the available information sources. The results of this study indicate that the proposed information surveillance system is effective. This study thus contributes to the market surveillance literature and has significant practical implications.

  12. Participatory Surveillance and Photo Sharing Practices

    DEFF Research Database (Denmark)

    Albrechtslund, Anders; Damkjaer, Maja Sonne; Bøge, Ask Risom

    2019-01-01

    -material perspective on photo-sharing practices. Information, Communication & Society, 19(4), 475–488. Sontag, S. (1977). On Photography. Picador. Steeves, V., & Jones, O. (2010). Editorial: Surveillance, Children and Childhood. Surveillance & Society, 7(3/4), 187–191....... that parents do not generally plan to store or organize their photos, and even less their children’s photos. This seems to indicate a shift from a pre-digital perception of photos as objects to be packaged, accumulated, framed etc. which can age and disappear (see Sontag, 1977) to something perceived less....... References: Albrechtslund, A. (2008). Online Social Networking as Participatory Surveillance. First Monday, 13(3). Fotel, T., & Thomsen, T. U. (2002). The Surveillance of Children’s Mobility. Surveillance & Society, 1(4), 535-554. Lobinger, K. (2016). Photographs as things–photographs of things. A texto...

  13. Microprocessor-based integrated LMFBR core surveillance

    International Nuclear Information System (INIS)

    Gmeiner, L.

    1984-06-01

    This report results from a joint study of KfK and INTERATOM. The aim of this study is to explore the advantages of microprocessors and microelectronics for a more sophisticated core surveillance, which is based on the integration of separate surveillance techniques. Due to new developments in microelectronics and related software an approach to LMFBR core surveillance can be conceived that combines a number of measurements into a more intelligent decision-making data processing system. The following techniques are considered to contribute essentially to an integrated core surveillance system: - subassembly state and thermal hydraulics performance monitoring, - temperature noise analysis, - acoustic core surveillance, - failure characterization and failure prediction based on DND- and cover gas signals, and - flux tilting techniques. Starting from a description of these techniques it is shown that by combination and correlation of these individual techniques a higher degree of cost-effectiveness, reliability and accuracy can be achieved. (orig./GL) [de

  14. Interleukin-21 mRNA expression during virus infections

    DEFF Research Database (Denmark)

    Holm, Christian; Nyvold, Charlotte Guldborg; Paludan, Søren Riis

    2006-01-01

    and activational effects of IL-21 on different leukocytes come into play in vivo in an immune response has so far not been fully investigated. We show here for the first time in vivo, that IL-21 mRNA is produced in the spleen when mice are challenged with herpes simplex virus type 2 (HSV-2) or lymphocytic...... choriomeningitis virus (LCMV). We show in HSV-2 challenged mice that this production takes place in CD4+ T cell fractions and is absent in CD4+ T cell-depleted fractions. We also show that the peak of IL-21 mRNA production in both the HSV-2 and LCMV-challenged mice coincides with the onset of the adaptive immune...

  15. Collagen mRNA levels changes during colorectal cancer carcinogenesis

    DEFF Research Database (Denmark)

    Skovbjerg, Hanne; Anthonsen, Dorit; Lothe, Inger M B

    2009-01-01

    BACKGROUND: Invasive growth of epithelial cancers is a complex multi-step process which involves dissolution of the basement membrane. Type IV collagen is a major component in most basement membranes. Type VII collagen is related to anchoring fibrils and is found primarily in the basement membrane...... zone of stratified epithelia. Immunohistochemical studies have previously reported changes in steady-state levels of different alpha(IV) chains in several epithelial cancer types. In the present study we aimed to quantitatively determine the mRNA levels of type IV collagen (alpha1/alpha 4/alpha 6......) and type VII collagen (alpha1) during colorectal cancer carcinogenesis. METHODS: Using quantitative RT-PCR, we have determined the mRNA levels for alpha1(IV), alpha 4(IV), alpha 6(IV), and alpha1(VII) in colorectal cancer tissue (n = 33), adenomas (n = 29) and in normal tissue from the same individuals...

  16. Peptide inhibitors of botulinum neurotoxin by mRNA display

    International Nuclear Information System (INIS)

    Yiadom, Kwabena P.A.B.; Muhie, Seid; Yang, David C.H.

    2005-01-01

    Botulinum neurotoxins (BoNTs) are extremely toxic. The metalloproteases associated with the toxins cleave proteins essential for neurotransmitter secretion. Inhibitors of the metalloprotease are currently sought to control the toxicity of BoNTs. Toward that goal, we produced a synthetic cDNA for the expression and purification of the metalloprotease of BoNT/A in Escherichia coli as a biotin-ubiquitin fusion protein, and constructed a combinatorial peptide library to screen for BoNT/A light chain inhibitors using mRNA display. A protease assay was developed using immobilized intact SNAP-25 as the substrate. The new peptide inhibitors showed a 10-fold increase in affinity to BoNT/A light chain than the parent peptide. Interestingly, the sequences of the new peptide inhibitors showed abundant hydrophobic residues but few hydrophilic residues. The results suggest that mRNA display may provide a general approach in developing peptide inhibitors of BoNTs

  17. Environmental surveillance at Los Alamos

    International Nuclear Information System (INIS)

    1979-04-01

    This report documents the environmental surveillance program conducted by the Los Alamos Scientific Laboratory (LASL) in 1978. Routine monitoring for radiation and radioactive or chemical substances is conducted on the Laboratory site and in the surrounding region to determine compliance with appropriate standards and permit early identification of possible undesirable trends. Results and interpretation of the data for 1978 on penetrating radiation, chemical and radiochemical quality of ambient air, surface and groundwater, municipal water supply, soils and sediments, food, and airborne and liquid effluents are included. Comparisons with appropriate standards and regulations or with background levels from natural or other non-LASL sources provide a basis for concluding that environmental effects attributable to LASL operations are minor and cannot be considered likely to result in any hazard to the population of the area. Results of several special studies provide documentation of some unique environmental conditions in the LASL environs

  18. The Nordic Obstetric Surveillance Study

    DEFF Research Database (Denmark)

    Colmorn, Lotte B.; Petersen, Kathrine B; Jakobsson, Maija

    2015-01-01

    by using International Classification of Diseases, 10th revision codes on diagnoses and the Nordic Medico-Statistical Committee Classification of Surgical Procedure codes. MAIN OUTCOME MEASURES: Rates of the studied complications and possible risk factors among parturients in the Nordic countries. RESULTS......OBJECTIVE: To assess the rates and characteristics of women with complete uterine rupture, abnormally invasive placenta, peripartum hysterectomy, and severe blood loss at delivery in the Nordic countries. DESIGN: Prospective, Nordic collaboration. SETTING: The Nordic Obstetric Surveillance Study...... (NOSS) collected cases of severe obstetric complications in the Nordic countries from April 2009 to August 2012. SAMPLE AND METHODS: Cases were reported by clinicians at the Nordic maternity units and retrieved from medical birth registers, hospital discharge registers, and transfusion databases...

  19. Ebola Surveillance - Guinea, Liberia, and Sierra Leone.

    Science.gov (United States)

    McNamara, Lucy A; Schafer, Ilana J; Nolen, Leisha D; Gorina, Yelena; Redd, John T; Lo, Terrence; Ervin, Elizabeth; Henao, Olga; Dahl, Benjamin A; Morgan, Oliver; Hersey, Sara; Knust, Barbara

    2016-07-08

    Developing a surveillance system during a public health emergency is always challenging but is especially so in countries with limited public health infrastructure. Surveillance for Ebola virus disease (Ebola) in the West African countries heavily affected by Ebola (Guinea, Liberia, and Sierra Leone) faced numerous impediments, including insufficient numbers of trained staff, community reticence to report cases and contacts, limited information technology resources, limited telephone and Internet service, and overwhelming numbers of infected persons. Through the work of CDC and numerous partners, including the countries' ministries of health, the World Health Organization, and other government and nongovernment organizations, functional Ebola surveillance was established and maintained in these countries. CDC staff were heavily involved in implementing case-based surveillance systems, sustaining case surveillance and contact tracing, and interpreting surveillance data. In addition to helping the ministries of health and other partners understand and manage the epidemic, CDC's activities strengthened epidemiologic and data management capacity to improve routine surveillance in the countries affected, even after the Ebola epidemic ended, and enhanced local capacity to respond quickly to future public health emergencies. However, the many obstacles overcome during development of these Ebola surveillance systems highlight the need to have strong public health, surveillance, and information technology infrastructure in place before a public health emergency occurs. Intense, long-term focus on strengthening public health surveillance systems in developing countries, as described in the Global Health Security Agenda, is needed.The activities summarized in this report would not have been possible without collaboration with many U.S and international partners (http://www.cdc.gov/vhf/ebola/outbreaks/2014-west-africa/partners.html).

  20. Nuclear law - Nuclear safety

    International Nuclear Information System (INIS)

    Pontier, Jean-Marie; Roux, Emmanuel; Leger, Marc; Deguergue, Maryse; Vallar, Christian; Pissaloux, Jean-Luc; Bernie-Boissard, Catherine; Thireau, Veronique; Takahashi, Nobuyuki; Spencer, Mary; Zhang, Li; Park, Kyun Sung; Artus, J.C.

    2012-01-01

    This book contains the contributions presented during a one-day seminar. The authors propose a framework for a legal approach to nuclear safety, a discussion of the 2009/71/EURATOM directive which establishes a European framework for nuclear safety in nuclear installations, a comment on nuclear safety and environmental governance, a discussion of the relationship between citizenship and nuclear, some thoughts about the Nuclear Safety Authority, an overview of the situation regarding the safety in nuclear waste burying, a comment on the Nome law with respect to electricity price and nuclear safety, a comment on the legal consequences of the Fukushima accident on nuclear safety in the Japanese law, a presentation of the USA nuclear regulation, an overview of nuclear safety in China, and a discussion of nuclear safety in the medical sector

  1. Regulation of mRNA translation influences hypoxia tolerance

    International Nuclear Information System (INIS)

    Koritzinsky, M.; Wouters, B.G.; Koumenis, C.

    2003-01-01

    Hypoxia is a heterogenous but common characteristic of human tumours and poor oxygenation is associated with poor prognosis. We believe that the presence of viable hypoxic tumor cells reflects in part an adaptation and tolerance of these cells to oxygen deficiency. Since oxidative phosphorylation is compromized during hypoxia, adaptation may involve both the upregulation of glycolysis as well as downregulation of energy consumption. mRNA translation is one of the most energy costly cellular processes, and we and others have shown that global mRNA translation is rapidly inhibited during hypoxia. However, some mRNAs, including those coding for HIF-1 α and VEGF, remain efficiently translated during hypoxia. Clearly, the mechanisms responsible for the overall inhibition of translation during hypoxia does not compromize the translation of certain hypoxia-induced mRNA species. We therefore hypothesize that the inhibition of mRNA translation serves to promote hypoxia tolerance in two ways: i) through conservation of energy and ii) through differential gene expression involved in hypoxia adaptation. We have recently identified two pathways that are responsible for the global inhibition of translation during hypoxia. The phosphorylation of the eukaryotic initiation factor eIF2 α by the ER resident kinase PERK results in down-regulation of protein synthesis shortly after the onset of hypoxia. In addition, the initiation complex eIF4F is disrupted during long lasting hypoxic conditions. The identification of the molecular pathways responsible for the inhibition of overall translation during hypoxia has rendered it possible to investigate their importance for hypoxia tolerance. We have found that mouse embryo fibroblasts that are knockout for PERK and therefore not able to inhibit protein synthesis efficiently during oxygen deficiency are significantly less tolerant to hypoxia than their wildtype counterparts. We are currently also investigating the functional significance

  2. Digital dashboard design using multiple data streams for disease surveillance with influenza surveillance as an example.

    Science.gov (United States)

    Cheng, Calvin K Y; Ip, Dennis K M; Cowling, Benjamin J; Ho, Lai Ming; Leung, Gabriel M; Lau, Eric H Y

    2011-10-14

    Great strides have been made exploring and exploiting new and different sources of disease surveillance data and developing robust statistical methods for analyzing the collected data. However, there has been less research in the area of dissemination. Proper dissemination of surveillance data can facilitate the end user's taking of appropriate actions, thus maximizing the utility of effort taken from upstream of the surveillance-to-action loop. The aims of the study were to develop a generic framework for a digital dashboard incorporating features of efficient dashboard design and to demonstrate this framework by specific application to influenza surveillance in Hong Kong. Based on the merits of the national websites and principles of efficient dashboard design, we designed an automated influenza surveillance digital dashboard as a demonstration of efficient dissemination of surveillance data. We developed the system to synthesize and display multiple sources of influenza surveillance data streams in the dashboard. Different algorithms can be implemented in the dashboard for incorporating all surveillance data streams to describe the overall influenza activity. We designed and implemented an influenza surveillance dashboard that utilized self-explanatory figures to display multiple surveillance data streams in panels. Indicators for individual data streams as well as for overall influenza activity were summarized in the main page, which can be read at a glance. Data retrieval function was also incorporated to allow data sharing in standard format. The influenza surveillance dashboard serves as a template to illustrate the efficient synthesization and dissemination of multiple-source surveillance data, which may also be applied to other diseases. Surveillance data from multiple sources can be disseminated efficiently using a dashboard design that facilitates the translation of surveillance information to public health actions.

  3. Cup regulates oskar mRNA stability during oogenesis.

    Science.gov (United States)

    Broyer, Risa M; Monfort, Elena; Wilhelm, James E

    2017-01-01

    The proper regulation of the localization, translation, and stability of maternally deposited transcripts is essential for embryonic development in many organisms. These different forms of regulation are mediated by the various protein subunits of the ribonucleoprotein (RNP) complexes that assemble on maternal mRNAs. However, while many of the subunits that regulate the localization and translation of maternal transcripts have been identified, relatively little is known about how maternal mRNAs are stockpiled and stored in a stable form to support early development. One of the best characterized regulators of maternal transcripts is Cup - a broadly conserved component of the maternal RNP complex that in Drosophila acts as a translational repressor of the localized message oskar. In this study, we have found that loss of cup disrupts the localization of both the oskar mRNA and its associated proteins to the posterior pole of the developing oocyte. This defect is not due to a failure to specify the oocyte or to disruption of RNP transport. Rather, the localization defects are due to a drop in oskar mRNA levels in cup mutant egg chambers. Thus, in addition to its role in regulating oskar mRNA translation, Cup also plays a critical role in controlling the stability of the oskar transcript. This suggests that Cup is ideally positioned to coordinate the translational control function of the maternal RNP complex with its role in storing maternal transcripts in a stable form. Published by Elsevier Inc.

  4. Nuclear power plant safety in Brazil

    International Nuclear Information System (INIS)

    Lederman, L.

    1980-01-01

    The Code of Practice for the Safe Operation of Nuclear Power Plants states that: 'In discharging its responsibility for public health and safety, the government should ensure that the operational safety of a nuclear reactor is subject to surveillance by a regulatory body independent of the operating organization'. In Brazil this task is being carried out by the Comissao Nacional de Energia Nuclear in accordance with the best international practice. (orig./RW)

  5. Follow-up radiological surveillance, Marshall Islands

    International Nuclear Information System (INIS)

    Greenhouse, N.A.

    1978-01-01

    The political approvals have been given for the return of Bikini and Enewetak Atolls to their original inhabitants. These two regions, which comprised the Pacific Nuclear Testing Areas from 1946 to 1958, are now being repopulated by their original inhabitants and their families. Recent assessments of internal and external exposure pathways at Bikini and Enewetak have indicated that doses and dose commitments in excess of current radiation protection guidelines are possible or even likely for persons living in these areas. Rongelap and Utirik Atolls, which were downwind of the 1954 Bravo event, also received significant fallout; potential radiological problems exist in these areas as well. In view of this prospect, follow-up environmental monitoring and personnel monitoring programs are being established to maintain our cognizance of radiological conditions, and to make corrective action where necessary. The unexpected finding of detectable amounts (above background) of plutonium in the urine of individuals at Bikini and Rongelap Atolls also raises the possibility of radiological problems in the long term from environmentally-derived plutonium via pathways which are not completely understood. This finding adds further impetus to the surveillance programs for an area where real radiological concerns for the general public are already known to exist. The continuing environmental and personnel monitoring programs which this paper describes are a necessary part of the BNL radiological safety program in the Marshall Islands, which is designed to do the following: (1) elucidate the internal exposure pathways; (2) define the external radiation environment; (3) assess the doses and dose commitments from radioactivity in the environment; (4) provide the feedback necessary to improve existing predictive modelling of radiological trends; and (5) suggest actions which will minimize doses via the more significant pathways. (author)

  6. Environmental surveillance at Hanford for CY-1981

    International Nuclear Information System (INIS)

    Sula, M.J.; McCormack, W.D.; Dirkes, R.L.; Price, K.R.; Eddy, P.A.

    1982-05-01

    Environmental surveillance activities performed by the Pacific Northwest Laboratory for 1981 are discussed. The results are summarized as follows: Radionuclide concentrations and radiation dose measurements were below applicable concentration guides and radiation dose standards. There was no difference detected between airborne radionuclide concentrations in samples collected near to and far from the Hanford Site. A difference in 129 I concentration in Columbia River water downstream compared to upstream was observed. Strontium-90 concentrations downstream remained similar to past years while reduced concentrations were observed in the upstream samples. In addition, 60 Co and 131 I were observed more frequently in the downstream river water samples than in the upstream samples. In all cases, the downstream radionuclide concentrations were small in comparison to DOE radionuclide concentration guides and state and EPA drinking water standards. Low concentrations of radionuclides attributed to operations were observed in wildlife collected onsite. Low concentrations of fallout radionuclides from worldwide atmospheric nuclear testing were observed in foodstuffs and in soil and vegetation; there was no indication of a Hanford contribution to radionuclide levels. The highest penetrating dose rates were in the vicinities of the 100N and 300 Areas. Dose rates at both locations resulted from the presence, within the operating areas, of contained radioactive materials. Nonradiological water quality parameters were all within State Water Quality Standards for the Columbia River. The maximum 50-year whole body dose commitment to an individual from effluents released in 1981 was calculated to be 0.4 mrem. The maximum 50-year dose to a single organ, considering all pathways was approximately 1.3 mrem to the bone, primarily due to 90 Sr in the Columbia River

  7. Nuclear material safeguards surveillance and accountancy by isotope correlation techniques

    International Nuclear Information System (INIS)

    Persiani, P.J.; Goleb, J.A.

    1981-01-01

    This paper presents the initial phase of the US study program and involves the computation of isotopic correlations for the LMFBR fuel cycle. The LWR fuel cycle phase of the study is currently in progress. The selection of the more safeguards effective functionals will depend not only on the level of reliability of isotope correlation technique (ICT) for verification, but also on the capability and difficulty of developing measurement methods. Performance characteristics of existing and proposed isotopic measurement techniques cover the general areas including assay and isotopic wet chemistry and NDA: (1) simultaneous multicomponent analysis techniques, (2) mass spectrometry, (3) x-ray fluorescence or densitometry with high flux monochromatic x-ray sources and high dispersion spectrometers, (4) passive and active neutron interrogation, (5) high level gamma-ray spectroscopy, (6) coulometry, and (7) potentiometry. The measurement capabilities and inherent limitations of these systems are to be evaluated in terms of total systems, operational mode, sample preparation requirements and consequent effect on dissolver solution representation, accuracy and precision estimates (if available), development status of the technique, and development requirements. The isotopic correlation technique shows considerable promise for use in verifying the initial isotopic composition and burnup of discharged assemblies based on the measured ratios of several key isotopes, obtained probably at the dissolver stage in reprocessing. This technique should, for example, easily be capable of indicating the exchange of a blanket assembly for a fuel assembly

  8. Expert system for online surveillance of nuclear reactor coolant pumps

    International Nuclear Information System (INIS)

    Gross, K.C.; Singer, R.M.; Humenik, K.E.

    1993-01-01

    An expert system for determining the operability of a specified pump is described comprising: a set of pumps of which the specified pump is a member; means for measuring physical parameters representative to the operations condition each pump of said set of pumps; means for acquiring data generated by said measuring means; an artificial-intelligence based inference engine coupled to said data acquiring means where said inference engine applies a sequential probability ratio test to statistically evaluate said acquired data to determine a status for the specified pump and its respective measuring means by continually monitoring and comparing changes in a specific operational parameter signal acquired from a plurality of measurement means; means for transferring said status generated by said interference engine to an output system

  9. System for unattended surveillance of nuclear reactor behavior

    International Nuclear Information System (INIS)

    Gonzalez, R.C.; Howington, L.C.

    1977-01-01

    A multivariate statistical pattern recognition system for reactor noise analysis is presented. The basis of the system is a transformation for decoupling correlated variables and algorithms for inferring probability density functions. The system is adaptable to a variety of statistical properties of the data, and it has learning, tracking, updating, and dimensionality reduction capabilities. System design emphasizes control of the false-alarm rate. Its abilities to learn normal patterns and to recognize deviations from these patterns were evaluated by experiments at the ORNL High-Flux Isotope Reactor. Power perturbations of less than 0.1% of the mean value in selected frequency ranges were readily detected by the pattern recognition system

  10. Genome-wide mapping of infection-induced SINE RNAs reveals a role in selective mRNA export.

    Science.gov (United States)

    Karijolich, John; Zhao, Yang; Alla, Ravi; Glaunsinger, Britt

    2017-06-02

    Short interspersed nuclear elements (SINEs) are retrotransposons evolutionarily derived from endogenous RNA Polymerase III RNAs. Though SINE elements have undergone exaptation into gene regulatory elements, how transcribed SINE RNA impacts transcriptional and post-transcriptional regulation is largely unknown. This is partly due to a lack of information regarding which of the loci have transcriptional potential. Here, we present an approach (short interspersed nuclear element sequencing, SINE-seq), which selectively profiles RNA Polymerase III-derived SINE RNA, thereby identifying transcriptionally active SINE loci. Applying SINE-seq to monitor murine B2 SINE expression during a gammaherpesvirus infection revealed transcription from 28 270 SINE loci, with ∼50% of active SINE elements residing within annotated RNA Polymerase II loci. Furthermore, B2 RNA can form intermolecular RNA-RNA interactions with complementary mRNAs, leading to nuclear retention of the targeted mRNA via a mechanism involving p54nrb. These findings illuminate a pathway for the selective regulation of mRNA export during stress via retrotransposon activation. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  11. Natural selection and algorithmic design of mRNA.

    Science.gov (United States)

    Cohen, Barry; Skiena, Steven

    2003-01-01

    Messenger RNA (mRNA) sequences serve as templates for proteins according to the triplet code, in which each of the 4(3) = 64 different codons (sequences of three consecutive nucleotide bases) in RNA either terminate transcription or map to one of the 20 different amino acids (or residues) which build up proteins. Because there are more codons than residues, there is inherent redundancy in the coding. Certain residues (e.g., tryptophan) have only a single corresponding codon, while other residues (e.g., arginine) have as many as six corresponding codons. This freedom implies that the number of possible RNA sequences coding for a given protein grows exponentially in the length of the protein. Thus nature has wide latitude to select among mRNA sequences which are informationally equivalent, but structurally and energetically divergent. In this paper, we explore how nature takes advantage of this freedom and how to algorithmically design structures more energetically favorable than have been built through natural selection. In particular: (1) Natural Selection--we perform the first large-scale computational experiment comparing the stability of mRNA sequences from a variety of organisms to random synonymous sequences which respect the codon preferences of the organism. This experiment was conducted on over 27,000 sequences from 34 microbial species with 36 genomic structures. We provide evidence that in all genomic structures highly stable sequences are disproportionately abundant, and in 19 of 36 cases highly unstable sequences are disproportionately abundant. This suggests that the stability of mRNA sequences is subject to natural selection. (2) Artificial Selection--motivated by these biological results, we examine the algorithmic problem of designing the most stable and unstable mRNA sequences which code for a target protein. We give a polynomial-time dynamic programming solution to the most stable sequence problem (MSSP), which is asymptotically no more complex

  12. [Effects of lipopolysaccharides extracted from Porphyromonas endodontalis on the expression of IL-1beta mRNA and IL-6 mRNA in osteoblasts].

    Science.gov (United States)

    Yang, Di; Li, Ren; Qiu, Li-Hong; Li, Chen

    2009-04-01

    To quantify the IL-1 beta mRNA and IL-6 mRNA expression induced by lipopolysaccharides (LPS)extracted from Porphyromonas endodontalis(P.e) in osteoblasts, and to relate P.e-LPS to bone absorption pathogenesis in lesions of chronical apical periodontitis. MG63 was treated with different concentrations of P.e-LPS(0-50 microg/mL) for different hours(0-24h). The expression of IL-1 beta mRNA and IL-6 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR).Statistical analysis was performed using one- way ANOVA and Dunnett t test with SPSS11.0 software package. The level of IL-1 beta mRNA and IL-6 mRNA increased significantly after treatment with P.e-LPS at more than 5 microg/mL (P<0.01)and for more than 1 hour (P<0.01), which indicated that P.e-LPS induced osteoblasts to express IL-1 beta mRNA and IL-6 mRNA in dose and time dependent manners. P.e-LPS may promote bone resorption in lesions of chronical apical periodontitis by inducing IL-1 beta mRNA and IL-6 mRNA expression in osteoblasts.

  13. Summary of environmental surveillance at Los Alamos during 1995

    International Nuclear Information System (INIS)

    1996-10-01

    Linking the Rio Grande Valley and the Jemez Mountains, New Mexico's Pajarito Plateau is home to a world-class scientific institution. Los Alamos National Laboratory (or the Laboratory), managed by the Regents of the University of California, is a government-owned, Department of Energy-supervised complex investigating all areas of modern science for the purposes of national defense, health, conservation, and ecology. The Laboratory was founded in 1943 as part of the Manhattan Project, whose members assembled to create the first nuclear weapon. Occupying the campus of the Los Alamos Ranch School, American and British scientists gathered on the isolated mesa tops to harness recently discovered nuclear power with the hope of ending World War II. In July 1945, the initial objective of the Laboratory, a nuclear device, was achieved in Los Alamos and tested in White Sands, New Mexico. Today the Laboratory continues its role in defense, particularly in nuclear weapons, including developing methods for safely handling weapons and managing waste. For the past twenty years, the Laboratory has published an annual environmental report. This pamphlet offers a synopsis that briefly explains important concepts, such as radiation and provides a summary of the monitoring results and regulatory compliance status that are explained at length in the document entitled Environmental Surveillance at Los Alamos during 1995

  14. Cost effectiveness of surveillance for GI cancers.

    Science.gov (United States)

    Omidvari, Amir-Houshang; Meester, Reinier G S; Lansdorp-Vogelaar, Iris

    2016-12-01

    Gastrointestinal (GI) diseases are among the leading causes of death in the world. To reduce the burden of GI diseases, surveillance is recommended for some diseases, including for patients with inflammatory bowel diseases, Barrett's oesophagus, precancerous gastric lesions, colorectal adenoma, and pancreatic neoplasms. This review aims to provide an overview of the evidence on cost-effectiveness of surveillance of individuals with GI conditions predisposing them to cancer, specifically focussing on the aforementioned conditions. We searched the literature and reviewed 21 studies. Despite heterogeneity of studies in terms of settings, study populations, surveillance strategies and outcomes, most reviewed studies suggested at least some surveillance of patients with these GI conditions to be cost-effective. For some high-risk conditions frequent surveillance with 3-month intervals was warranted, while for other conditions, surveillance may only be cost-effective every 10 years. Further studies based on more robust effectiveness evidence are needed to inform and optimise surveillance programmes for GI cancers. Copyright © 2016 Elsevier Ltd. All rights reserved.

  15. Standardized rendering from IR surveillance motion imagery

    Science.gov (United States)

    Prokoski, F. J.

    2014-06-01

    Government agencies, including defense and law enforcement, increasingly make use of video from surveillance systems and camera phones owned by non-government entities.Making advanced and standardized motion imaging technology available to private and commercial users at cost-effective prices would benefit all parties. In particular, incorporating thermal infrared into commercial surveillance systems offers substantial benefits beyond night vision capability. Face rendering is a process to facilitate exploitation of thermal infrared surveillance imagery from the general area of a crime scene, to assist investigations with and without cooperating eyewitnesses. Face rendering automatically generates greyscale representations similar to police artist sketches for faces in surveillance imagery collected from proximate locations and times to a crime under investigation. Near-realtime generation of face renderings can provide law enforcement with an investigation tool to assess witness memory and credibility, and integrate reports from multiple eyewitnesses, Renderings can be quickly disseminated through social media to warn of a person who may pose an immediate threat, and to solicit the public's help in identifying possible suspects and witnesses. Renderings are pose-standardized so as to not divulge the presence and location of eyewitnesses and surveillance cameras. Incorporation of thermal infrared imaging into commercial surveillance systems will significantly improve system performance, and reduce manual review times, at an incremental cost that will continue to decrease. Benefits to criminal justice would include improved reliability of eyewitness testimony and improved accuracy of distinguishing among minority groups in eyewitness and surveillance identifications.

  16. Improved margin utilization through the use of beacon power distribution surveillance

    International Nuclear Information System (INIS)

    Miller, R. Wade; Boyd, William A.

    2002-01-01

    Core Operations, including fuel cycle costs, can be significantly improved when state of the art surveillance techniques are employed for core power distribution monitoring. Core power distribution monitoring and Technical Specification surveillance are major operational issues at PWR's, particularly in plants with movable in core detectors. Even plants with fixed in core detectors do not always make use of the continuous data that is available. The BEACON TM system (Best Estimate Analysis of Core Operations - Nuclear) is a core monitoring and operational support package developed by Westinghouse for use in PWR plants with fixed or movable in core detectors. BEACON is a real time core monitoring system, which uses existing core instrumentation data and an on-line neutronics model to provide continuous monitored of the core power distribution information. With this information available the BEACON system can be used to continuously monitor core power margin for the plant Tech Spec surveillance requirements and for plant operational guidance

  17. Long-term surveillance plan for the Collins Ranch Disposal Site, Lakeview, Oregon

    International Nuclear Information System (INIS)

    1993-12-01

    This long-term surveillance plan (LTSP) for the Lakeview, Oregon, Uranium Mill Tailings Remedial Action (UMTRA) Project disposal site describes the surveillance activities for the Lakeview (Collins Ranch) disposal cell, which will be referred to as the Collins Ranch disposal cell throughout this document. The US Department of Energy (DOE) will carry out these activities to ensure that the disposal cell continues to function as designed. This final LTSP was prepared as a requirement for acceptance under the US Nuclear Regulatory Commission (NRC) general license for custody and long-term care of residual radioactive materials. This LTSP documents whether the land and interests are owned by the United States or an Indian tribe, and details how the long-term care of the disposal site will be carried out. It is based on the DOE's Guidance for Implementing the UMTRA Project Long-term Surveillance Program (DOE, 1992a)

  18. Long-term surveillance plan for the Mexican Hat Disposal Site, Mexican Hat, Utah

    International Nuclear Information System (INIS)

    1996-02-01

    This plan describes the long-term surveillance activities for the Uranium Mill Tailings Remedial Action (UMTRA) Project disposal site at Mexican Hat, Utah. The U.S. Department of Energy (DOE) will carry out these activities to ensure that the disposal site continues to function as designed. This long-term surveillance plan (LTSP) was prepared as a requirement for acceptance under the U.S. Nuclear Regulatory Commission (NRC) general license for custody and long-term care of residual radioactive material (RRM). This LTSP (based on the DOE's Guidance for Implementing the UMTRA Project Long-term Surveillance Program), documents the land ownership interests and details how the long-term care of the disposal site will be accomplished

  19. Long-term surveillance plan for the Collins Ranch disposal site, Lakeview, Oregon

    International Nuclear Information System (INIS)

    1994-08-01

    This long-term surveillance plan (LTSP) for the Uranium Mill Tailings Remedial Action (UMTRA) Project Collins Ranch disposal site, Lakeview, Oregon, describes the surveillance activities for the disposal cell. The U.S. Department of Energy (DOE) will carry out these activities to ensure that the disposal cell continues to function as designed. This final LTSP was prepared as a requirement for acceptance under the U.S. Nuclear Regulatory Commission (NRC) general license for custody and long-term care of residual radioactive materials. This LTSP documents whether the land and interests are owned by the United States and details how long-term care of the disposal site will be carried out. It is based on the DOE's Guidance for Implementing the UMTRA Project Long-term Surveillance Program (DOE, 1992a)

  20. Long-term surveillance plan for the Green River, Utah, disposal site

    International Nuclear Information System (INIS)

    1997-06-01

    The long-term surveillance plan (LTSP) for the Green River, Utah, Uranium Mill Tailings Remedial Action (UMTRA) Project disposal site describes the surveillance activities for the Green River disposal cell. The U.S. Department of Energy (DOE) will carry out these activities to ensure that the disposal cell continues to function as designed. This final LTSP was prepared as a requirement for acceptance under the U.S. Nuclear Regulatory Commission (NRC) general license for custody and long-term care of residual radioactive materials (RRM). This LTSP documents whether the land and interests are owned by the United States or an Indian tribe and details how the long-term care of the disposal site will be carried out. The Green River, Utah, LTSP is based on the DOE's Guidance for Implementing the UMTRA Project Long-term Surveillance Program (DOE, 1992a)